LOCUS       DASSZH010000001         4466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_886386, whole genome shotgun sequence.
ACCESSION   DASSZH010000001 DASSZH010000000
VERSION     DASSZH010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886386"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2369)
                     /gene="topA"
                     /locus_tag="VFN42_00005"
     CDS             complement(<1..2369)
                     /gene="topA"
                     /locus_tag="VFN42_00005"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006558567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET8995024.1"
                     /translation="MTDVVVVESPAKAKTINRYLGSGYTVLASFGHVRDLPPKDGSVR
                     PEQDFAMDWESDSRGDRQVSAIAKALKGARTLYLATDPDREGEAISWHVKAMLGEKKA
                     LKGIEVRRVTFNEITRNAIRTAMAAPRDLDQPLIEAYLARRALDYLVGFTLSPVLWRK
                     LPGSRSAGRVQSVALRLICEREAEIEVFRPREYWTVEAEFLTPAGAPFTARLTHLDGK
                     RLDQFDLNNEALAMRAKAAVEAGKFTVGSVERKRVKRNPPPPFTTSTLQQEASRKLGF
                     GAQQTMRVAQQLYEGIDIGGESVGLITYMRTDGVQMAREAVAAIRDHVKDAYGGNYLP
                     AAPREYQSKIKNAQEAHEAIRPTDVTRTPDAVAHELNADQRRLYELVWKRAVASQMQS
                     AELDQVTVEVTDTKGLKLRATGSIVAFDGFLKLYREDTDDTAEGAAADEDNRMLPPMA
                     ERDPLKRGLVNALQHFTQPPPRYSEASLVKKLEELGIGRPSTYASILSVLQDRKYVRL
                     EKRRFIPEDRGRLVTAFLVSFFETYVDTNFTAGLEEKLDDISAGQLDWREVMHAFWDE
                     FSKAVDQTKDLKISDVIAALDQELGPHFFPSREDGSDPRVCPACGNGRLGLKLGRYGS
                     FIGCSNYPACQYTRRLAIETGDDNGETLKEGMRVLGTHPDTGDEITVRRGPYGLYVQQ
                     GEANAEDKKAPKPKRTSLPRGMDGEQITLEQALGLLSLPRVVGTHPETGEKIEVGIGR
                     FGPYVRMGAVYGSLERDDNVLSLGMNRAMDLLAKKLASVRTLGAHPGDK"
     gene            complement(2375..3517)
                     /gene="dprA"
                     /locus_tag="VFN42_00010"
     CDS             complement(2375..3517)
                     /gene="dprA"
                     /locus_tag="VFN42_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HET8995025.1"
                     /translation="MSTTCSPLHDIVARLRLARVEGVGPIAYRRMLQRFRSATAALEA
                     LPELLRSGGRTSVPEIPSQTQAEDELARLDQIGARLLVLDTQGYPPLLSLLEDAPPVL
                     GVLGDPGVLAQPAVALVGARNASANGQRLAERLAAELAHGIVVISGLARGIDAAAHQG
                     AMATGRTVAAVAGGLDIVYPAEHAALQRRIAENGAVVAEAPLGTAPQARHFPRRNRII
                     AGLSLGVVVVEAALRSGSLITTRIAQDLGREVFAVPGSPLDPRSHGSNDLIRQGAVLT
                     ESAADVLAHLPDLPAHSLFSQLPQAHAGLAESPPEPLAPAMNLTNIKEQVIDLLSPSP
                     TAVDELVRRCQFSSAAVMAALLELELAGRVEALPGNRVALLARKAH"
     gene            complement(3627..3902)
                     /locus_tag="VFN42_00015"
     CDS             complement(3627..3902)
                     /locus_tag="VFN42_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995026.1"
                     /translation="MTPPSNVALRFIVEEDPRLVQALNQAYAEADPDGGLDKPEREAL
                     QDVIGRHFTGRHWPRKASMEGTRRFMADLQRAMAAAGWKVDLFAVAA"
     gene            complement(4069..>4466)
                     /locus_tag="VFN42_00020"
     CDS             complement(4069..>4466)
                     /locus_tag="VFN42_00020"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995027.1"
                     /translation="EITGEDITAKDFRTWAATNLAALAFRELAAFESQAKAKKNTVRV
                     VEAVAKMLGNTPAICRKCYIHPAIFDGYLDGSLLETLQRRARARLADPGHGLKAEEAA
                     VIAFLSRRLDERTASPQKIQLQTGKIGRA"
BASE COUNT          660 a         1503 c         1546 g          757 t
ORIGIN      
        1 tccttgtcgc ccggatgcgc gcccagcgtg cgcacgctgg ccagcttctt cgccagcaga
       61 tccattgcgc ggttcatgcc caggctcagc acgttgtcgt cgcgctccag cgagccgtac
      121 accgcgccca tgcgcacata ggggccgaag cggccgatgc cgacctcgat cttttccccg
      181 gtctccggat gcgtgccgac cacgcgcggc agcgacagca ggccgagcgc ctgctccagc
      241 gtgatctgct cgccatccat gccgcggggc agggaggtgc gcttcggttt cggcgccttc
      301 ttgtcctcgg cgttcgcctc gccctgctgc acgtacagcc cgtatggacc acggcgcacg
      361 gtgatctcat caccggtgtc cgggtgcgtg cccagcaccc gcatgccttc cttcagcgtc
      421 tcgccgttgt cgtcgccggt ctcgatcgcc aggcggcggg tgtactggca ggcgggatag
      481 ttggagcagc cgatgaagct gccgtagcgg cccagtttca ggcccaggcg gccgttgccg
      541 caggccgggc agacgcgggg atcgctgccg tcctcacggc tgggaaagaa gtgtggcccc
      601 agctcctgat ccagcgcggc gatcacgtcg cttattttga ggtccttggt ctggtccacc
      661 gccttggaga actcatccca gaaggcatgc atcacttccc gccagtcgag ctggccggcg
      721 gagatgtcgt ccagcttctc ctcgagcccg gcggtgaagt tggtgtcgac gtaggtctcg
      781 aagaagctga ccaggaaggc ggtgaccagc cggccgcgat cctccggaat gaagcggcgt
      841 ttctccagcc ggacgtattt gcggtcctgc agcaccgaca ggatcgacgc gtaggtggag
      901 gggcgaccga tgccgagttc ctccagcttc ttcaccagcg acgcttcgga gtagcggggc
      961 ggcggctggg tgaaatgctg cagcgcattc accaggccac gcttcagcgg atcgcgttcc
     1021 gccatcggcg gcagcatgcg gttgtcttcg tccgctgccg ccccctcggc cgtatcgtca
     1081 gtgtcctcgc gatacagctt caggaacccg tcaaacgcga cgatcgagcc ggtggcgcgc
     1141 agcttcaggc ccttggtgtc ggtcacctcc acagtgacct ggtcgagttc cgccgactgc
     1201 atctgcgaag cgacggcgcg cttccacacc agctcgtaca gccgccgctg gtcggcattc
     1261 agctcatgcg ccaccgcgtc cggcgtgcgg gtcacgtcgg tggggcggat cgcctcatgc
     1321 gcttcctgcg cgttcttgat cttgctctgg tattcgcgtg gcgcggcggg caggtagttg
     1381 ccgccatacg catccttgac atggtcgcgg atcgccgcca ccgcctcgcg ggccatctgc
     1441 acgccatcgg tccgcatata ggtgatcagg ccgacgctct cgccgccgat atcgatgcct
     1501 tcgtagagct gctgcgcgac gcgcatcgtc tgctgcgcac cgaagcccag cttgcggctg
     1561 gcttcctgct gcagggtgga ggtggtgaag ggcggcggcg gattgcgctt gacccgcttg
     1621 cgctccaccg agccgacggt gaacttgccg gcttcgaccg cggccttggc gcgcatcgcc
     1681 agggcttcgt tgttcaggtc gaactggtcc agccgcttgc cgtccaggtg ggtcagccgg
     1741 gcggtgaagg gcgcgccggc cggtgtgagg aactcggcct caacggtcca gtattcgcgc
     1801 ggacggaaga cctcgatctc ggcttcgcgc tcgcagatca gccgcagcgc caccgactgc
     1861 acgcgccccg cggaacggct gccgggcagc ttgcgccaca gcaccgggga cagggtgaag
     1921 cccaccaggt agtccagcgc gcggcgggcg aggtaggcct cgatcagcgg ctggtccagg
     1981 tcgcgcgggg ccgccatggc ggtgcggatc gcgttgcggg tgatctcgtt gaaggtgacg
     2041 cggcggactt cgatgccctt cagcgccttc ttctcgccca gcatcgcctt gacgtgccag
     2101 gagatcgcct cgccctcgcg atccgggtcg gtggcgaggt acagcgtgcg ggctcccttc
     2161 agcgccttgg caatggcgct gacctggcga tcgccacggg agtcggactc ccagtccatg
     2221 gcgaagtcct gctccggccg gacgctgccg tccttcggcg gcaggtcgcg cacatggccg
     2281 aaactggcca gcacggtgta gccgctgccg aggtagcggt tgatggtctt ggccttggcg
     2341 ggggattcga cgacgacgac gtcagtcatg attcctagtg agccttccgc gcgagcagcg
     2401 caacccggtt gcccggcagc gcttcgacgc ggccggcgag ttccagttcc agcagagccg
     2461 ccatgacggc agccgatgag aactggcagc gccgcacaag ctcgtcaact gcggttggac
     2521 tggggctaag caaatcaatg acttgttctt tgatgttggt cagattcatg gcgggtgcga
     2581 gcggttccgg cggtgattcg gccagcccgg catgggcctg cggaagctgg ctgaacagcg
     2641 aatgggcggg caggtcgggc aaatgcgcca gcacgtcggc ggcgctttcg gtcagcacgg
     2701 cgccctggcg gatcaggtca ttgctgccat gcgagcgcgg atcgagcggc gaaccgggta
     2761 ctgcgaagac ttcccgcccg aggtcctggg caatgcgggt ggtgatcagg ctgccggaac
     2821 gcaacgccgc ttccaccacc accacgccga gggacaggcc ggcgatgatc cggttgcgcc
     2881 gggggaaatg ccgcgcctgg ggggcggtgc cgagcggcgc ctcggccacc acggcaccgt
     2941 tctcggcgat gcggcgctgc agggcggcat gttcggcggg gtaaacgatg tccagcccgc
     3001 cggcgactgc cgcgacggtg cggcccgtgg ccatggcacc ctggtgcgcg gcggcatcga
     3061 taccgcgggc aagccccgag atcaccacga tgccatgcgc cagttcggcg gcgagccgct
     3121 ccgcgaggcg ctgcccgttg gccgaggcgt tgcgggcgcc gaccagcgcc acggcgggct
     3181 gcgccagcac cccggggtcg cccagcacgc ccaggaccgg gggcgcgtct tccagcagcg
     3241 acagcagcgg tggatagcct tgcgtgtcca gcaccagcag gcgcgcaccg atctggtcca
     3301 gccgggcgag ttcgtcctcc gcctgggttt ggctggggat ctccggcacg ctggtgcgcc
     3361 ccccggagcg cagcaattcc ggcagggcct ccagcgccgc ggtggcggaa cggaagcgct
     3421 gcagcatgcg ccggtaggcg atcggaccga cgccctcgac ccgggcgagg cgcagccgcg
     3481 cgacgatgtc gtgcagcgga gagcaggtgg tggacacgcg acgctcccat ggcatggagg
     3541 cccgccgccg ggcgggttcg cgcgctattg caccacagca attaccaacg ccggcttaac
     3601 gccggctgcc ggggcgtctg atgccctcag gcggcgaccg cgaacagatc gaccttccag
     3661 ccggccgccg ccatcgcgcg ctgcagatcc gccatgaagc gccgcgtccc ttccatcgag
     3721 gctttgcgcg gccagtgccg gccggtgaaa tgccggccga tcacgtcctg caatgcctcg
     3781 cgctcgggct tgtccagccc gccgtccggg tcggcctcgg cataggcctg gttcagcgcc
     3841 tgcaccagcc gaggatcctc ctcgacgatg aaacgcaagg cgacgttcga gggcggcgtc
     3901 atgtgacgcc gctcgagttc ccaccggcag caggccaggg gcgctggaag cagggtaacc
     3961 aaggttggct ccgcagattt caggtcggcg caacgcgcca taatgctgac acatggtgcc
     4021 gaggaaggca ggcttccagg gtgggctaga aacacattca ggcgggagtc aagcccgtcc
     4081 gattttccct gtctggagct ggattttctg tggggaggcg gtacgttcat ccaggcggcg
     4141 gctgaggaag gcgatcaccg cggcttcctc agccttcagc ccgtggccgg ggtccgccag
     4201 ccgggcgcgg gcccggcgct ggagcgtctc cagcagcgac ccatccaggt agccgtcgaa
     4261 gatcgccggg tggatatagc atttgcggca gatcgccggg gtgttgccca gcatcttcgc
     4321 cactgcctcc accacccgaa cggtattctt cttcgccttg gcctggcttt cgaaggccgc
     4381 cagctcgcgg aaggccaggg ccgccagatt ggtcgccgcc caggtgcgga agtccttggc
     4441 ggtgatgtcc tcgccggtga tctcct
//
LOCUS       DASSZH010000002         2708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1414794, whole genome shotgun sequence.
ACCESSION   DASSZH010000002 DASSZH010000000
VERSION     DASSZH010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414794"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="VFN42_00025"
     CDS             complement(<1..817)
                     /locus_tag="VFN42_00025"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HET8995028.1"
                     /translation="MTTPQPRPEILTINPYIGGESDLPGRNRTIKLSSNEGAFGVPPG
                     AQEAIRAVTENVFRYPDGGADALRAALGKRWGLDPARIVCGAGSDDLIYQLCLSYGGP
                     GRDIVMSAHGFAIYQIAGTYAGSRVIKVPERNLTADLDAMLAAVSPATRLMFLANPNN
                     PTGSMVPSGEVARLRERLPPEVLLVLDSAYAEYVTHADFDPGAALVDASGTTVMTRTF
                     SKVFGLGGMRIGWCYAPPSVVDVLNRVRGPFNVSVAAQAAAIAALQEPGWVEKG"
     gene            complement(814..1722)
                     /locus_tag="VFN42_00030"
     CDS             complement(814..1722)
                     /locus_tag="VFN42_00030"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HET8995029.1"
                     /translation="MSSPAPTADASFPPAANDEPPPAPDGWPGGLPALRAELDRIDND
                     LHDLLMQRARVVEAVARSGKRSAWRPGREAQIIRRLLSRHTGHLPPQSVYRIWRELLA
                     ATTAMQGGFRVAVCERDPATGFTQLAREHFGALTPLRTHGGPAQAIGEVGAGIAAVAV
                     LPMPSEHENWWSALLHREEPRIHIAARLPFWATRLEGNPALQALVVGPAAPDASEHDR
                     SLIGLELEQDISRDRLSAAFAAAGFGQAAVWVLRRDPGAPLAQALVETEGHVTEDDPR
                     LDRLTGVHRRPVVLGGYAVPVGGPEV"
     gene            complement(1841..2356)
                     /gene="folK"
                     /locus_tag="VFN42_00035"
     CDS             complement(1841..2356)
                     /gene="folK"
                     /locus_tag="VFN42_00035"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET8995030.1"
                     /translation="MILVSIGANLATPDGTPPLETCREAAAALDRLPGLRLRGLSRWY
                     RTAPVLAEGQDAASQPFYVNGVADLVSQSDIEIDPAWLLAELMRLEAAAGRVRSSPNA
                     PRPLDLDIIAIGDLLRDHPDPILPHPRAHQRAFVLVPLAEVAPGWVHPRLRRSVEALI
                     SELPPQDIAPL"
     gene            complement(2353..>2708)
                     /locus_tag="VFN42_00040"
     CDS             complement(2353..>2708)
                     /locus_tag="VFN42_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995031.1"
                     /translation="AGFVAVPQYGPASELARSMPADMPVLGVDPRWAFFMLPAPQTGA
                     GPGLLVWPGSGADPDPAQWPGLRMVGGAGRGTPEEPIEVFRLYSVSRLPAGVAAARLP
                     SARTAIESGSIERRQ"
BASE COUNT          452 a          947 c          937 g          372 t
ORIGIN      
        1 ggcccttctc cacccaaccc ggctcctgca gggcggcgat tgccgccgcc tgcgccgcga
       61 cggagacgtt gaacggcccg cgcacccggt tcagcacatc cacgaccgag gggggcgcat
      121 agcaccagcc gatccgcatg ccgcccagcc cgaagacctt ggagaaggtg cgggtcatca
      181 ccgtggtgcc actggcatcc accagcgccg cgcccgggtc gaaatccgca tgcgtgacgt
      241 attcggcata ggcggagtcg agcaccagca gcacctccgg cggcagccgc tcgcgcagcc
      301 gagcgacttc gccggacggc accatcgacc cggtgggatt gttcggattg gccaggaaca
      361 tcagccgcgt ggccggcgac accgcggcca gcatggcgtc cagatcggcg gtcaggttgc
      421 gctccggcac cttgatcacc cggctgccgg cgtaggtgcc cgcaatctgg tagattgcga
      481 agccatgcgc cgacatgacg atgtcgcgtc ccggcccgcc atagctcagg catagctggt
      541 agatcaggtc gtcggacccc gcaccgcaga cgatgcgcgc cgggtccagc ccccagcgct
      601 tgcccagcgc cgcgcgcagt gcgtctgcgc caccatctgg atagcggaat acgttttcgg
      661 tcaccgcgcg gatggcttcc tgcgcaccag gaggcacgcc aaacgcacct tcgttggagg
      721 acaacttgat cgtccggttg cggcccggca gatccgactc gccgccgata tagggattta
      781 tggtgagtat ctcggggcgc ggctgcggag tggtcacacc tccggccctc cgacaggcac
      841 cgcgtatccg cccagcacca ccggccggcg atgcacgccg gtcaaccgat ccagacgcgg
      901 gtcgtcttcc gtcacatggc cctcggtttc caccagcgcc tgagccagcg gcgcaccggg
      961 atcccgccgc agtacccaca cggcggcctg gccgaacccg gcggcggcaa atgcggcgga
     1021 gagccggtcg cggctgatgt cctgctccag ttcaaggccg atgaggctgc ggtcatgttc
     1081 ggaggcgtcg ggcgcggcgg gtcccaccac cagggcctgc aaagccgggt tgccctccag
     1141 ccgtgtggcc cagaagggca agcgtgcggc gatgtggata cgcggttcct cgcggtgcag
     1201 cagtgcggac caccagtttt cgtgctctga cggcatcggc agcacggcga cggcagcgat
     1261 acccgcgccc acctcgccga tcgcctgggc gggaccgcca tgcgtgcgca acggcgtcag
     1321 cgccccgaaa tgctcccgcg cgagctgggt gaaaccggtg gccgggtcgc gctcgcagac
     1381 cgccacgcgg aaaccaccct gcatcgcggt ggtggcagcc agcagctccc gccagatgcg
     1441 atagaccgat tgcgggggca ggtggccggt gtgacgcgag agcagccggc ggatgatctg
     1501 ggcctcccgc cccggacgcc aggcagagcg cttgccggag cgcgccaccg cctccaccac
     1561 gcgggcacgc tgcatcagca ggtcgtgcag gtcgttatcg atacggtcca gttcggcgcg
     1621 gagcgcaggc aggccaccgg gccagccatc cggtgccggc ggcggctcgt cattggccgc
     1681 cggggggaac gaggcatcag cagttggggc agggctggac attctcttgg ggccggtttg
     1741 ggtcggaaca gatagccgat gcgcccgaag gtgcaaaccc ggtgcttgcg gaccgcgtga
     1801 cgcaaaatgc ccggaaagcg gaatggcacc gtacatcttg tcaaagaggc gcgatgtcct
     1861 gcggcggcag ttcggagatc agggcctcga cactgcgccg tagccgggga tgcacccagc
     1921 ctggcgccac ctcggccagg ggcaccagga caaaggcgcg ctgatgcgcc cgcggatgcg
     1981 gcaggatcgg atccgggtgg tcccgtagca gatcgccgat ggcgataatg tccaggtcaa
     2041 gtgggcgggg cgcgttcggg ctgctgcgga cccggccagc ggctgcctcc agccgcatca
     2101 gctcggccag caaccaggct gggtcgatct cgatgtcgga ttgcgaaacc aggtccgcaa
     2161 cgccgttgac gtagaagggc tgggaggcgg catcctgccc ttccgccagc acaggcgcag
     2221 tgcggtacca gcgcgacagc ccgcgcaggc gcagccccgg caagcgatca agcgccgccg
     2281 ctgcctcgcg gcaggtttcc agcggtgggg tgccatcagg cgtggccaga ttggcgccga
     2341 tcgaaaccag gatcattgcc gccgctcgat cgagcccgac tcaatggcgg tgcgcgctga
     2401 cggcaaccgt gcggccgcca cacccgccgg cagccggctc accgaataaa gccggaacac
     2461 ctcgatgggt tcctccggcg tgccgcgtcc ggccccgccg accatccgca ggcccggcca
     2521 ttgcgccgga tccgggtcgg caccggagcc cggccagacc agcaggcctg gaccagcgcc
     2581 ggtttgtggc gccggcagca tgaagaaagc ccagcgcgga tcgactccca gcaccggcat
     2641 atccgccggc atggagcggg ccaattcgct ggccggcccg tattggggga ccgcgacaaa
     2701 cccggcgt
//
LOCUS       DASSZH010000003         3365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_460268, whole genome shotgun sequence.
ACCESSION   DASSZH010000003 DASSZH010000000
VERSION     DASSZH010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460268"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VFN42_00045"
     CDS             complement(<1..516)
                     /locus_tag="VFN42_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthetase family protein"
                     /protein_id="HET8995032.1"
                     /translation="MHIFRDWRDLPGEARGATVALGNFDGVHLGHAALVRAAHAARPD
                     APRAVLTFEPHPREVFRPDDPPFRLTLSAERADALAALGVELLYELPFSPDFSLMPAE
                     IFVSEVLHQGVGARHLVCGPDFAFGQRRGGDTRLLAQRAEALGMGLTLVPLVKDAQGP
                     LSSTRIRRALQD"
     gene            complement(516..938)
                     /locus_tag="VFN42_00050"
     CDS             complement(516..938)
                     /locus_tag="VFN42_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET8995033.1"
                     /translation="MSTGTFFEDLQIGQTASLGKTITEADILMYAAVSLDSNPVHLNA
                     EAAKQSMFGERIAHGMLSAGLISAVLGTQLPGPGTIYLGQSLRFTRPVKIGDTVTATV
                     EVTELQAERKRATLKTVCTVAGKTVIEGVATVQVPSRG"
     gene            complement(1020..1361)
                     /locus_tag="VFN42_00055"
     CDS             complement(1020..1361)
                     /locus_tag="VFN42_00055"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HET8995034.1"
                     /translation="MTTHPGGSRRALLLCAAGLVCIPVAGSAAEPAQAIVGIHNFAFQ
                     PAVLTVPRGTTVTWVNHDDSPHSIVLASLNVHSHPLDTDGRFSFRFEQPGAFAYRCGL
                     HPHMQGTVVVQ"
     gene            complement(1374..2288)
                     /locus_tag="VFN42_00060"
     CDS             complement(1374..2288)
                     /locus_tag="VFN42_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET8995035.1"
                     /translation="MSDNSDRRGFLKCMAWAGSAMVWSVAGGVPRSRLIGSAEAATQE
                     FSFVQVSDSHLGFNKPVNTDPTATLQAALAQVKALPGQPAFMLHTGDITHLSQPAAFD
                     TAQQMMRQTGLAVHTVPGEHDILEEDGKSYLHRFGKGSKGDGWYSFDAGGVHFIGLIN
                     VVDLQGNGLGALGHEQLEWLEHDVAGLSASTPVVVFAHVPLWVVYEPWGWGTADGAQA
                     LRYLRRFGSVTVLNGHIHQVMQKVEGHVAFHTARSTAFPQPAPGQAKSPGPMAVPPGA
                     LRSYLGVARVSLVSAASPLAIVDSPLMG"
     gene            2445..2999
                     /locus_tag="VFN42_00065"
     CDS             2445..2999
                     /locus_tag="VFN42_00065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET8995036.1"
                     /translation="MPGQDRTRRFELLAMPHLDAAYNFARWLVGNPADAEDVVQDAYL
                     RAFRYFDSFHGGDFRVWLLCIVRNSFLTWLKANRSNRLVFQPDLPDDGVPETVWGLPP
                     ADPETLLLRQIDGDTLMRLLHALPAEYREVLLLRELEELSYKAIAAIIGVPPGTVMSR
                     LSRARSALRTLWLRETAREAAGEV"
     gene            3013..>3365
                     /locus_tag="VFN42_00070"
     CDS             3013..>3365
                     /locus_tag="VFN42_00070"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995037.1"
                     /translation="MLPRLADRAIDPLRRWLLTRHLAQCSACAAQFEDLLAMQAALRT
                     ALPYHRAPPGLAARIGAALPQEAPPASGVWRRRAPWRMAGSGLAGVVAGVALTLLVMP
                     RPAPRQALIGAAVAD"
BASE COUNT          481 a         1234 c         1126 g          524 t
ORIGIN      
        1 gtcctgcagt gcgcgccgga tgcgggtgga ggacagcggc ccttgcgcgt ccttcaccag
       61 cggcaccagc gtcagcccca tgcccagcgc ctccgcccgt tgcgccagca ggcgggtgtc
      121 gccgccccgg cgttggccga acgcgaagtc cgggccgcac accaggtgcc gggcgccgac
      181 cccctggtgc agcacctcgg agacgaagat ctccgccggc atcagactga agtccgggct
      241 gaacggcagc tcgtacagca gttccacccc cagcgccgcc aacgcgtcgg cgcgttcggc
      301 ggacagcgtc agccggaacg gcgggtcgtc cgggcggaac acctcccgcg gatgcggctc
      361 gaaggtcagc accgcgcgcg gcgcgtccgg ccgggcggca tgggcggcgc gcaccagcgc
      421 cgcatggccg aggtggacgc cgtcgaaatt ccccagcgcc accgtggcgc cgcgcgcctc
      481 cccgggcagg tcgcgccagt cgcgaaagat atgcattagc cgcgcgaggg gacctgcacc
      541 gtggccaccc cctcgatcac cgtcttgccg gccaccgtgc agacggtctt cagcgttgcc
      601 cgcttgcgct cggcttgcag ttcggtgact tccaccgtcg ccgtcacggt gtcgccgatc
      661 ttcaccggcc gggtgaagcg cagcgactgg cccagataga tcgtgcccgg cccgggcagc
      721 tgggtgccca gtaccgcgga gatcaggccg gcggacagca tgccgtgggc gatccgctcg
      781 ccaaacatgg actgcttcgc cgcctcggcg ttcaggtgca ccggattgct gtccaggctg
      841 accgccgcgt acatcaggat gtccgcctcg gtgatggtct tgcccaggct ggcggtctgg
      901 ccgatctgca gatcctcgaa gaaggtgccg gtgctcatgg gtgcgtcccg ttgctgaccg
      961 ggcaccttct tgggcgaagg gggcggggga atcaaccgcg gccggtcccg cccgcgccgc
     1021 tactgcacca ccaccgtccc ctgcatgtgc gggtgcaggc cgcagcgata cgcgaacgca
     1081 cccggctgtt cgaaccggaa ggagaaccgc ccatccgtgt cgagcggatg cgaatgcacg
     1141 ttcagactcg ccagcacgat gctgtgcgga ctgtcgtcgt ggttcaccca ggtcaccgtg
     1201 gtgccgcgcg gcacggtcag caccgccggc tggaacgcga aattgtggat gcccacgatg
     1261 gcctgcgccg gctccgccgc actgccggcg acagggatgc acaccaggcc tgccgcacac
     1321 agcagcagcg cacgccggga cccgcccgga tgtgttgtca tggccatctc cgctcagccc
     1381 atcaacggcg agtcgacaat ggccagcggg ctcgcggccg agaccaggct gacccgcgct
     1441 acgcccagat agctgcgcag cgcgcccggc ggcaccgcca tcgggccggg gctcttcgcc
     1501 tgtccgggtg ccggctgcgg aaatgccgtg gaccgcgcgg tatggaacgc gacatgtccc
     1561 tccaccttct gcatcacctg gtggatgtgg ccgttcagca ccgtcaccga gccgaagcgc
     1621 cgcaggtagc gcagcgcctg cgcgccgtcc gcggtgcccc agccccatgg ctcgtacacc
     1681 acccagagcg gcacatgcgc gaacaccacc accggcgtgc tggcgctgag tcccgccacg
     1741 tcatgctcca gccattcgag ctgttcgtgc cccagcgcgc cgagcccgtt gccttgcaga
     1801 tccaccacgt tgatcaggcc gatgaaatgc acgccgccgg cgtcgaagct gtaccagccg
     1861 tcgcctttgc tgcccttgcc gaagcggtgc aggtagctct tgccatcctc ttccagaatg
     1921 tcgtgttcgc ccggcacggt atgcacggcc aggccggtct gccgcatcat ctgctgtgcg
     1981 gtgtcgaatg ccgccggctg cgacagatgc gtgatatcac cggtgtgcag catgaacgcc
     2041 ggctgtccgg gcagcgcctt cacctgcgcc agcgctgctt gcagcgtcgc ggtcggatcg
     2101 gtgttgaccg gcttgttgaa gcccagatgg ctatcgctga cctgcacgaa gctgaactcc
     2161 tgcgtcgccg cctcggcgct gccgatcagc cgggagcgcg gcacgccgcc ggccaccgac
     2221 cacaccatcg cgctgccggc ccaggccatg catttcagga atccacgccg gtcgctgtta
     2281 tcgctcatga ctcactcccc gttgcatcgg cggcctccat ccggcgcgcc tgcccgctct
     2341 accgcgccgc cgccgcgcct attccgcagg cagaaaaatt cgccgcggcg ggaataaccg
     2401 gccgggtttg ccggtagact gcgcagatcg gggaaccgca gggcttgcca ggccaggacc
     2461 ggacacgacg cttcgagctg ctcgcgatgc cgcatctgga tgcggcgtac aatttcgcgc
     2521 gctggctggt cggcaacccg gcggatgccg aggatgtggt gcaggacgcc tacctgcgcg
     2581 ccttccgcta tttcgactcg ttccacggcg gcgatttccg tgtctggctg ctctgcatcg
     2641 tgcgcaacag tttcctcacc tggctcaagg ccaaccgcag caaccgcctg gtgttccagc
     2701 cggacctgcc ggacgacggc gtgccggaaa ccgtctgggg cctgccgcca gccgatccgg
     2761 agaccctgct gctgcggcag atcgacggcg acacgctgat gcgcctgttg cacgccctgc
     2821 cggcggaata tcgggaggtc ctgctgctgc gcgagctgga ggagctgtcg tacaaggcga
     2881 tcgccgccat catcggcgtg ccgcccggta ccgtgatgtc gcgcctgtcg cgtgcccggt
     2941 ccgcgttgcg gacgctgtgg ctgcgggaaa ccgcgcggga ggccgccggt gaagtgtgac
     3001 cgcagccgaa ccttgctgcc gcgcctggcc gaccgtgcca tcgacccgct gcgtcggtgg
     3061 ctgctgaccc gccatctcgc gcagtgctct gcctgcgccg cgcagttcga ggacttgctg
     3121 gcgatgcagg ccgccttgcg caccgccctg ccataccacc gcgccccgcc gggcctggcg
     3181 gcccgcattg gcgccgccct gccgcaggag gcgccgcccg catccggcgt ctggcggcgc
     3241 cgagcaccat ggcgcatggc cggatcgggc ttggccggcg tggtcgccgg cgtggcgctg
     3301 acgctgctgg tcatgccgcg gccggcgccg cgccaggcac tgatcggcgc cgccgtcgcc
     3361 gatca
//
LOCUS       DASSZH010000004         2411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1073914, whole genome shotgun sequence.
ACCESSION   DASSZH010000004 DASSZH010000000
VERSION     DASSZH010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073914"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1748)
                     /locus_tag="VFN42_00075"
     CDS             complement(<1..1748)
                     /locus_tag="VFN42_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HET8995038.1"
                     /translation="MAAARSLIRIAVNVAIDGVLAAASVPLAGWIADPAGAALHPLWL
                     LPAGAAALLLAGVPFRLSLQYWRFAGLEDLLTVAASSATGAGLFLLLRHLTGVVSPNP
                     AFPLVHALTLLLLLGTPRVLYRRMQIARASGRSPHARAPGILLIGTGEDADLFLRALA
                     HDRRQPFRVEGLLSLGSRQTGRRIQGHPILGPLSELSAVLKRLEQEDRLPDILVIASP
                     DVSGPALSGLIELAERHRVAVRRAPRPTALGPADTAKPVELKPVVIEDLLNRPQVPLD
                     RDGMARLIQGRRVIVTGAGGTIGSELARQVAAFGPETLILLDNGEYALWQIDLELAET
                     HPSVPRQSLLADVRDEARLRAVFETCRPELVFHAAALKHVPMVEDNPLEGLLTNAAGT
                     RHVADAARAVDALAMVLISTDKAVNPTSVMGASKRLAEMYCQALDVSARVSGQGMACV
                     TVRFGNVLGSTGSVVPLFQRQLARGGPLTVTHPDMQRYFMTVREAVGLVLQASVLRTG
                     GAALPAGEEGGIFVLDMGKPVKIVDLARQMIRLAGLQPDSDVEIRFTGLRPGEKLYEE
                     LFHGKEPPVPTGHPGLL"
     gene            1894..>2411
                     /locus_tag="VFN42_00080"
     CDS             1894..>2411
                     /locus_tag="VFN42_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8995039.1"
                     /translation="MQNPLRRMVPQPSRAAVFLGLLAVAAWLDNAIPESPPLPETRPS
                     SDTPKPAMPQQDAKPWYRPFEGSYAASLTSAIVALSPFIVVTTAHVMFGRQVVQDVHA
                     SRTASSIIAGLSVAGYAWGALLGGDLVQRFRQRRMFIIAELLFALGCLLAAIAHAAPI
                     YAAGRVLTGFGTG"
BASE COUNT          380 a          878 c          815 g          338 t
ORIGIN      
        1 agcaggccgg ggtgaccggt gggcaccggc ggctccttgc cgtggaacag ttcctcatac
       61 aacttttccc cgggccgcag gccggtgaag cggatttcca cgtcgctgtc cggttgcagt
      121 ccggccaagc ggatcatctg ccgcgccagg tcgacgatct tcactggctt gcccatgtcc
      181 agcacgaaga tgcccccctc ctcgccggcc ggcagcgccg caccgccggt acgcagcacg
      241 ctggcctgca gcaccagtcc caccgcttcc cgcaccgtca tgaagtagcg ctgcatgtcg
      301 ggatgggtca cggtcagcgg tccgccccgc gcgagctgcc gctggaacag cgggaccacc
      361 gagccggtgc tgcccagcac gttgccaaac cgcacggtga cgcatgccat gccctggccg
      421 ctcacgcggg cggaaacatc cagcgcctgg caatacatct ccgccaggcg cttcgaggct
      481 cccatcacgc tggtcggatt caccgccttg tcggtggaga tcagcaccat cgccagcgcg
      541 tccacggcgc gggcggcgtc ggccacgtgg cgggtgccgg cggcattggt cagcaggcct
      601 tcgagcggat tgtcctccac catcggcacg tgcttcagcg ctgccgcgtg gaacaccagc
      661 tcggggcggc aggtttcgaa caccgcgcgg agacgggcct cgtcgcggac atcggccagt
      721 agcgactggc gcggcaccga gggatgggtc tccgccagtt ccagatcgat ctgccacagc
      781 gcgtattcgc cgttgtccag caggatcagc gtctccgggc cgaaggcagc cacctgccgc
      841 gccaactcgc tgccgatcgt gccgccggcc ccggtgacga tcacccgccg gccctggatc
      901 agccgcgcca tgccatcgcg atccagcggc acttgcggac ggttcagcag atcctcgatg
      961 acaaccggct tcaactcgac gggcttggcg gtatcggccg gccccagcgc ggtggggcgg
     1021 ggcgctcggc gcaccgcgac acgatggcgc tccgccagtt cgatcagccc ggacagggcc
     1081 gggccggaga cgtcgggcga ggcgatcacc aggatgtccg gcaaccggtc ctcctgttcc
     1141 agccgcttca gtaccgccga tagctcgctg agcggaccca ggatcggatg cccctgaatg
     1201 cgccgcccgg tctgccggga gccaagcgac agcagcccct ccacccggaa cggctggcgg
     1261 cggtcatgcg ccagggcacg caggaacagg tcggcgtcct cgccggtgcc gatcagcagg
     1321 atgccgggcg cccgggcgtg cggcgagcgg ccgctggcgc gggcgatctg catgcggcgg
     1381 tagagcaccc gtggcgtgcc gagcagcagc agcagggtca gcgcatgcac cagcggaaag
     1441 gccgggttgg gcgataccac gccggtcaga tggcgcagca gcaggaacaa cccggcaccg
     1501 gtggcgctgg acgccgccac ggtcagcaga tcctccagcc ccgcgaagcg ccagtattgc
     1561 agggagagcc ggaacggcac ccccgccaga agcagcgccg ccgcccccgc cggtagcagc
     1621 cagagcggat gcaaggcggc gcccgccgga tcggcgatcc agccggcaag cggaacggac
     1681 gccgccgcca gaacgccatc gatggcgaca ttgacggcga tgcgaatgag cgagcgggcc
     1741 gcggccatgc cgcccctata ccccagaggc gggacggctc ctaccctggt caaacgggcc
     1801 agcacgccgg ccgggccgaa gcctcacgcc gtcgggttgc agggcgatgc aactccaccc
     1861 gcacccaccc gttgggtcgc ggtgccagga cccatgcaaa atccgctgcg gcgcatggtg
     1921 ccgcaaccgt cccgcgcggc cgttttcctg ggcctgctgg ccgtggccgc atggctggac
     1981 aacgccatcc ccgaatcgcc gcccctgccg gaaacccgtc cttccagcga cacaccaaag
     2041 cctgccatgc cgcagcagga cgccaagccg tggtaccgcc cctttgaggg cagctacgcc
     2101 gcctcgctga ccagcgccat cgtggcgctg tcgcccttca ttgtggtgac gaccgcccac
     2161 gtgatgttcg gccggcaggt ggtgcaggac gtgcatgcgt cgcgcaccgc atcgtcgatc
     2221 atcgccggcc tgtcggtcgc tggctatgcc tggggtgccc tgctgggtgg cgacctggtg
     2281 cagcgattcc gccagcggcg catgttcatc attgccgaac tgctgttcgc gctcggctgc
     2341 ctgctcgcgg cgatcgcgca tgcggcaccg atctatgccg cggggcgtgt gctaacggga
     2401 ttcggcaccg g
//
LOCUS       DASSZH010000005         2574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_153468, whole genome shotgun sequence.
ACCESSION   DASSZH010000005 DASSZH010000000
VERSION     DASSZH010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153468"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VFN42_00085"
     CDS             complement(<1..493)
                     /locus_tag="VFN42_00085"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8995040.1"
                     /translation="MPLSASETPLAPSDPYDVIVLGAGAAGMTAAAAAAARGLSVLLL
                     EKTAQVGGTTSVSGGMVWVPNNAKAAAVGRPDSLDAARMYLEATVSGTHNAAVRDHFL
                     TRAAGAIDWLEAQRIVHLQPVALYPDYYPDLPGATLGGRVLEPVPFDARRLGREFALL
                     RPPL"
     gene            complement(606..1514)
                     /locus_tag="VFN42_00090"
     CDS             complement(606..1514)
                     /locus_tag="VFN42_00090"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8995041.1"
                     /translation="MQPAGVSRMIGANRQEETQMGKLTGKVAIVTGASRGIGETIAEL
                     FAAEGAKVVCAARTLNEGEHRMLEGSLARTVARIRDAGGEAVPVTADVSTEADCVALV
                     QQARAAFGPIDILVNNAALNYYLPTADYPTNRWVRCFAVNVHGPFMLAKEVLQDMIPR
                     RSGAIVNISSGAAIGPGRGPYQDMTVRGGTMYGATKAALERFTQGLAQELAQYEGISV
                     SAVSPSRVVPTPGTIHHKLVTGMDDPKGEHPSFMARAALLLASEPAAAVNGRVTYSQQ
                     ILKEFGWIAQGGGRGVDSPGSGYSQI"
     gene            1566..2069
                     /locus_tag="VFN42_00095"
     CDS             1566..2069
                     /locus_tag="VFN42_00095"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HET8995042.1"
                     /translation="MQKLSLGLIAAGLIGLAATPGLAAPSSQSKAFASNAAQSGLAEV
                     QEGQLAQQKASSPQVRRFGQTLEQDHSAANQRLQEIAQQEKITLPAQPSHKQRSELKK
                     LRRLSGQQFDRAFAQEEVKDHQKAIAQFEREARSGKDATMKTFARDTLPVLRKHLQIA
                     QSLASNR"
     gene            complement(2078..>2574)
                     /locus_tag="VFN42_00100"
     CDS             complement(2078..>2574)
                     /locus_tag="VFN42_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HET8995043.1"
                     /translation="AAGIFTALVPGSMISMTAATLLSKNLIGLARPNATDAQTVGLAK
                     LMVPVIMLIGVYFTLTGNSTIVTMLLVGYSFVTQLFPTVIASLLPRNPVTAAGAFAGI
                     LTGVVVAAILTFTHATIGTLIPGVPEVVRDLNVGIIALIANIVVLAVVSAITRIPAAQ
                     AAAR"
BASE COUNT          429 a          898 c          870 g          377 t
ORIGIN      
        1 gcaagggcgg acgcagcagg gcaaactccc tgcccagacg gcgcgcatcg aacggcaccg
       61 gttccagcac cctgccgccc agcgtggcgc cgggcaggtc cggataatag tccgggtaca
      121 gtgccaccgg ctgcagatgc acgatgcgct gcgcctccag ccagtcgatc gcgccggcgg
      181 cgcgggtgag gaaatgatcg cgcaccgccg cattgtgcgt gccggagacc gtggcctcca
      241 ggtacatgcg ggcggcgtcc agcgaatccg gccggcccac cgccgccgcc ttggcgttgt
      301 tcggcaccca caccatgccg ccggaaaccg aagtggtgcc gccgacctgc gcggtcttct
      361 ccaacagcag caccgacaat ccgcgcgcgg cggcggcggc ggcagccgtc atgccggcgg
      421 caccggcacc gagcacgatg acgtcatacg ggtcggacgg tgcgagcggc gtctcggatg
      481 cggaaagcgg catcggacga tcctgcctgg aaggccggac gatcgttgct catgcacgca
      541 atgcacgcaa ccgccgtgat ccagccgcca gccgcggcgc gccagtcgga cgcgcagcag
      601 ccggttcaga tctgcgaata tcccgacccg ggactgtcca cgccgcgccc gccgccctgc
      661 gcgatccagc cgaattcctt gaggatctgc tggctgtagg tcacccgccc gttcaccgcc
      721 gcggccggtt cgctggccag cagcagggcg gcgcgcgcca tgaaggacgg gtgctcaccc
      781 ttcggatcgt ccatgccggt gaccagcttg tggtggatcg tgccgggcgt gggcaccacg
      841 cgcgagggcg ataccgcgct gacgctgatc ccctcgtatt gcgccagttc ctgcgccagg
      901 ccctgggtga agcgctccag cgccgccttg gtcgcgccgt acatcgtgcc gccgcgcacc
      961 gtcatgtcct ggtatggccc gcgtcccggg ccgatcgccg cgccggaact gatgttgacg
     1021 atcgcgccgg agcgccgcgg gatcatgtcc tgcaacactt ccttcgccag catgaatggc
     1081 ccatgcacgt tcaccgcgaa gcagcgcacc cagcggttgg tcgggtagtc cgcggttggc
     1141 aggtagtagt tcagcgctgc attgttcacc aggatatcga tcggcccaaa ggccgcccgc
     1201 gcctgctgca ccagggcgac gcaatcggcc tcggtggaga cgtcggcggt gaccggaacc
     1261 gcctcgccgc ccgcatcgcg gatgcgcgcc acggtgcggg cgagggagcc ttccagcatg
     1321 cggtgctcgc cctcgttcag cgtgcgcgcg gcgcacacca ccttggcgcc ttccgcggcg
     1381 aacagttcgg cgatggtctc gccgatgccg cggctggctc cggtgacgat ggcaaccttg
     1441 ccggtgagct tgcccatttg cgtttcctcc tggcggttgg cgccgatcat ccggctgacg
     1501 ccggccggct gcaacccggc ttcccagccg gcgttgcaac ggcaccgcaa cgcaggagtg
     1561 ccttcatgca gaagctgtca ctgggcctga tcgccgccgg cctgatcggc ttggccgcca
     1621 cgccggggct tgccgcgcca tcctcccagt ccaaggcctt tgccagcaac gcggcgcaat
     1681 ccggcctggc tgaagtgcag gaaggccagc tcgcccaaca gaaggcgagt tcaccccagg
     1741 tgcggcgctt cggccagacc ttggagcagg accatagcgc ggcgaaccag cggctccagg
     1801 aaatcgccca gcaggagaag atcaccctgc cggcgcaacc ctcccataag cagcgctccg
     1861 agctgaagaa gctgcgcaga ctgtcaggcc agcagtttga ccgcgccttc gcgcaggagg
     1921 aggtcaagga ccaccagaag gccatcgcgc agttcgaacg cgaggcgcgg tccggcaagg
     1981 atgccacgat gaagaccttc gcgcgtgaca cgctgccggt gctgcgcaag catctgcaga
     2041 tcgcgcagtc gcttgcgagc aaccgctagg cggcggatta ccgcgccgcc gcctgcgcgg
     2101 ccgggatgcg ggtgatcgca ctgaccaccg ccagcacgac gatgttggcg atcagtgcga
     2161 tgatcccgac attcaggtcg cgcaccactt ccggcacgcc ggggatcagc gtgccgatcg
     2221 tggcatgggt gaaggtcagg atcgccgcga ccaccacgcc ggtcaggatg ccggcgaagg
     2281 cgccggccgc ggtgaccgga ttgcgcggca gcaggctggc gatcaccgtc gggaagagct
     2341 gggtgacgaa gctgtagcca accagcagca tggtcacgat ggtcgagttg ccggtgaggg
     2401 tgaaatacac gccgatcagc atgatgaccg gcaccatcag cttggccagc ccgacggtct
     2461 gcgcatcggt cgcattcggc cgcgccaggc cgatcaggtt cttcgacagc agcgtggcgg
     2521 ccgtcatgct gatcatcgag cccggcacca gcgcggtgaa gatgccggcg gcac
//
LOCUS       DASSZH010000006         2391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_119386, whole genome shotgun sequence.
ACCESSION   DASSZH010000006 DASSZH010000000
VERSION     DASSZH010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119386"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="VFN42_00105"
     CDS             <1..740
                     /locus_tag="VFN42_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET8995044.1"
                     /translation="YADFNCLVLPPDAEQKQLDYVMLSDIWPTGWHATELAGLKPGES
                     IVIYGAGPVGMMAAYSAMVKGASKVIVVDRHPDRLKLVEQIGAIPVDDSKTDPVEQVL
                     KLTDGVGAHRGCECVGYQCHDHHGHEASNLTMNRLVASVRPTGGLGVVGVFVPQDPGS
                     KDPLEQHGQIAFDMGTFWFKGQFMRTGQANVKAYNRYLMNLIHAGRAQPSFLVSHTLK
                     LDQAPDAYGHFDERDAGWTKVVLRPAA"
     gene            772..1659
                     /locus_tag="VFN42_00110"
     CDS             772..1659
                     /locus_tag="VFN42_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET8995045.1"
                     /translation="MTQQVPVDDRAETGIEIPEENVFEIAPDVAYRRLALVNIVLVGQ
                     PQGVGARWVLIDAGLPGTAGIIARTAAARFTGASPPAAIILTHGHFDHVGALRTLAER
                     WDVPVYAHPLEMPYLDGRSAYPPPDPGVGGGIFSRMSTLFPRAPVDVSRWLRPLPDDG
                     GVPHMPGFRWLHTPGHAAGHVSFFRDADRLLIAGDAFITTAQESAYAVTVQAPELHGP
                     PMYFTHDWQAAATSVRALAALEPEIAVCGHGRAMRGEKMRLGLHRLANEFEAVAVPHG
                     GRYVAHPARPQDGSAYPGT"
     gene            complement(1797..>2391)
                     /locus_tag="VFN42_00115"
     CDS             complement(1797..>2391)
                     /locus_tag="VFN42_00115"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET8995046.1"
                     /translation="LAPGLVPGLVPGLAPDPALEAGAAPPYLTAPAARVARWSAWLDR
                     SSPCRRVGLVCSGDPGHPRDHARSIPLKAFHDLLDLPGISFVLVQTELRAADRVTFEA
                     AANLRFPGAALTDYADTAGLLAGLDLLISVDTSAAHLAGALGRPVWTLLPYCPDYRWQ
                     LGRPDTGWYPSMRLYRQDRPGEWNNVIARVRDDLAAR"
BASE COUNT          405 a          830 c          797 g          359 t
ORIGIN      
        1 cgtatgccga cttcaactgc ctggtgctgc cgcccgatgc ggagcagaaa caactcgact
       61 acgttatgct gtccgacatc tggccgaccg gctggcacgc gacggagctg gccggtctga
      121 aacccgggga gagcattgtc atctacggcg ccggcccggt cggaatgatg gcggcctatt
      181 cggcgatggt gaagggtgca tcgaaggtca tcgtggtgga ccgccacccg gaccggctga
      241 agctggtgga acagatcggc gccatcccgg tggatgacag caagaccgac ccggtcgaac
      301 aggtgctgaa gctgaccgac ggcgtcggcg cccatcgtgg ctgtgagtgc gtcggctatc
      361 aatgccatga ccaccatggg catgaggcgt cgaacctgac gatgaaccgc ctggtcgcct
      421 cggtccgccc gaccggtggc ctgggcgtcg tcggcgtgtt cgtgccgcag gaccccggca
      481 gcaaggatcc gctggagcag catggccaga tcgccttcga catgggcacg ttctggttca
      541 agggacagtt catgcggacc ggccaggcca atgtgaaggc ctacaaccgc tacctgatga
      601 acctgatcca cgccggccgc gcgcaaccgt ccttcctggt gtcgcacacc ctgaagctgg
      661 accaggcgcc ggacgcctac ggccacttcg atgagcgtga tgcgggctgg acgaaagtcg
      721 tcctgcggcc ggcggcctga cctgtcatcc gccacaagga aggagaacac catgacgcag
      781 caggttccgg tggacgaccg ggccgaaacc ggcatcgaga tccccgagga gaacgtcttc
      841 gagatcgcgc cggacgtcgc ctaccgtcgg ctcgccctgg tgaacatcgt gcttgtcggc
      901 cagccgcaag gcgtcggcgc acgctgggtg ctgatcgacg ccgggctgcc cggcaccgcc
      961 gggatcatcg cccgcaccgc cgccgcccgg ttcaccggcg cctcgccccc ggccgcgatc
     1021 atcctgacgc acgggcactt cgaccatgtc ggcgccttgc gaaccctcgc cgaacgctgg
     1081 gacgtgccgg tgtatgccca tccgttggag atgccgtatc tcgacggccg cagcgcctac
     1141 ccgccgccgg acccgggggt cggcggcggc atcttctccc gcatgtccac gctgttcccg
     1201 cgcgcgccgg tcgatgtgtc gcgctggctg cggccgctgc cggatgacgg cggcgtgccg
     1261 cacatgcccg gctttcgctg gctgcacaca cccggccatg cggcgggcca tgtctccttc
     1321 ttccgcgacg ccgaccggct gctgatcgcc ggtgacgcct tcatcaccac ggcgcaggaa
     1381 tccgcctatg ccgtcaccgt gcaggcgccg gagctgcatg ggccgccgat gtatttcacc
     1441 catgactggc aggccgccgc gacatcggtc cgggcgttgg cggcgctgga gccggagatc
     1501 gcagtgtgcg gccacggccg cgcgatgcgg ggtgaaaaga tgcggctggg cctgcatcgc
     1561 ctggcgaatg agttcgaagc cgtcgccgtt ccccacggcg gccgctacgt cgcccacccc
     1621 gctcggccgc aggacggatc ggcctatccg ggcacctgac cacacgtcag accggcgccc
     1681 ggccgaagcg cgataccagg gcatgatcgc ctgaggctga aagccgtaag gcgtgaatca
     1741 tgcgctcaaa caatgacttc taggtcatga tcgggtcaac ctgacccgaa catgatctag
     1801 cgagcggcca ggtcatcccg gacgcgcgcg attacgttgt tccactcgcc gggccggtcc
     1861 tgccggtaca ggcgcatgga aggataccag cctgtgtcgg gccggccgag ttgccagcgg
     1921 tagtctggac aatagggcag caaagtccat accggccggc ccagcgcgcc cgccagatgt
     1981 gccgctgacg tatcgaccga gatcagtagg tccaggcctg ccagcaggcc ggcggtatcg
     2041 gcatagtcgg tcagagcggc ccccgggaag cgcaggttgg ccgccgcttc gaacgtgacc
     2101 cggtcggcag cgcgcaactc ggtctgcacc agcacgaacg aaataccggg cagatccagc
     2161 aggtcatgga acgccttcag cgggatcgac cgcgcatgat cgcgcgggtg acccggatcg
     2221 ccgctgcaca cgagtcccac ccggcgacag ggcgaggacc ggtccagcca cgcggaccag
     2281 cgcgccaccc gcgcggcggg cgccgtcagg tagggcggcg ctgcaccagc ttccaacgcc
     2341 gggtctggcg ccaaccctgg cactaaccct ggcactaacc ctggcgccag c
//
LOCUS       DASSZH010000007         3882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_750107, whole genome shotgun sequence.
ACCESSION   DASSZH010000007 DASSZH010000000
VERSION     DASSZH010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750107"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VFN42_00120"
     CDS             <1..295
                     /locus_tag="VFN42_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995047.1"
                     /translation="FAGSSGAGPQRPCRRTVRGGHHPEGSVILTQQLRGTVVRSRGGW
                     PGRASGSLMDLLPGLTERPSKALPSDAMIIAGLEDDTARFGARGIGGKVGARI"
     gene            315..734
                     /locus_tag="VFN42_00125"
     CDS             315..734
                     /locus_tag="VFN42_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8995048.1"
                     /translation="MRALLIDSDALAAHAIERMLQSSGILTDQAHSSQEALQLARRRE
                     YDVILIDPMLTDVDGRDLLDRVQAASSAPLLALSASERARARAKAFRLGANDCIDKPF
                     GRVELVARVKALASGRVPSPLPRPAQGRLLEIAPTAA"
     gene            868..>3882
                     /locus_tag="VFN42_00130"
     CDS             868..>3882
                     /locus_tag="VFN42_00130"
                     /inference="COORDINATES: protein motif:HMM:NF037694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HET8995049.1"
                     /translation="MRSLPNGVFLCLLAVFLARAPVALAQTAPESGAQIQLFIGQTLP
                     GLAADAARLRFDLTRRHTALSAAAPGERAAAEAELRKAEAAHDWNAARAAAERRIRLG
                     NAGDPALWLSLAAAELKDPTGSAKTALQAAYIGFAERRNATDEPALTRQALLLMRQAL
                     GRLGDHLAEIRLLGEIVQAWPDDATAKAQLQRAVSRYGFAVRKIDTEPQSFPARACIA
                     FTLPLPADEALHPGDYVSTEPAIAALAVSAEQGRLCLSGLPPGATTVVKLHPGLPGIA
                     GTRLERMLTIRISLANRQPSLLADPTHFIIPASQPPAIGFASVNISKLKVKIARVAER
                     SLRGFLSNHPLLNPGAYASGLNGENAPVVFTGSAGVPDFVRNRLMHTVLPLADVMQKP
                     GLYAVSLAPGDGTPDEDGSLNLVQLVLRTNLAPTTWRGADGLTVQLRRFTDAGLVAGA
                     TVALIARDNAVLATADTGADGMAHFAAPLLAGPGGEAPAALHITGADGDFTLVNLDAS
                     PFDLTDRGVSGRPQPKPIDPYIWLDRGIYRPGETLHVGALLRSPDLKPLDVPLHLIVR
                     RPGGQVFADQVVHWTADSSLVEPITLSPGAQAGSWTIALAMSDKDPALASRSFTVAAF
                     VPARLAVDFAQHPPLAPGRIDHQPVTVRFLYGAPGANLTGTASVHITADATPFAAFKD
                     YRFGLHDEITNAPLLQPTLPQTDAKGATSVPIDLTHLPDATHALKATVSVTINDPSGR
                     AVTRSTTLPIVPKAALIGIKPDFTGGAVDQGAKPGFELAAVAPDGKPVAMQVDLSLVR
                     QDYEWEVIWNASVARWHFSYIDRPVLTRTLTIEPGQPYHLELPALPYGRYRLRLVQHA
                     GGLAASSAIFYSGWVTSDNPAVPARLTVARDKRIYAAGDTAHLHITAPFAGRATLVLA
                     NSKVISTRDFDLSKDGADLTVPVSADWGAGAYAVVHVYRPADGKTPPDRAVGLTWLGL
                     TPGDRKLPVTIAAQKLYRPDRDVDFAVHTTPGA"
BASE COUNT          555 a         1409 c         1326 g          592 t
ORIGIN      
        1 cttcgccggg tcgtccggtg ccggtccgca gcgtccttgt cgccgaaccg tgaggggcgg
       61 ccaccatccg gaaggctctg ttatcctcac acaacaattg cgcggcacgg tcgtccgcag
      121 ccgcggcggc tggcctggcc gcgccagcgg ctcactcatg gacttgttac caggactgac
      181 cgaacgcccc tcgaaggctt tgccgtccga tgctatgata attgctggct tggaggacga
      241 taccgcccgc ttcggcgcgc gcggtatcgg cggcaaggtc ggggcacgga tatagtcggg
      301 cggagcatga tcccatgcgg gcactgctga ttgattctga tgccttggcc gcgcatgcga
      361 tcgaacggat gttgcaatcc agtggcatcc tgacggacca ggcgcattcc tcgcaggagg
      421 cactgcagct ggcccggcgc cgcgaatacg acgttatcct cattgacccg atgctgacgg
      481 atgtggatgg gcgggacctg ctggatcggg tgcaggccgc cagctccgcg ccgcttctcg
      541 cgctgtcggc ctccgagcgg gcgcgcgcca gggcgaaggc gttccggctt ggcgccaatg
      601 actgcatcga caagccattc ggacgtgtgg agttggtggc acgcgtgaag gctctggcga
      661 gcgggcgggt tccgtcgccg ctcccgcgac ccgcccaggg gcgcctgctg gagattgccc
      721 ccaccgcggc ctgaccgtcc tgcccccggc ggccgcaagc aaaccgctgc gcctcttccg
      781 cgacgcgccg ccagttcggt atgcataggc accgtcccgc gccagccccg cttcggccgg
      841 gcgcagacct cgagccggag cagcccgatg cgttctctgc cgaacggcgt gtttctctgc
      901 ctgctggcgg ttttcctcgc ccgcgctccc gtcgccctgg cgcagaccgc accggaaagc
      961 ggcgcgcaga tccagctttt catcggacag acactgccgg gcctcgcggc ggacgccgcg
     1021 cgcctgcgct tcgacctcac gcggcgccac accgcgctgt ccgccgcggc gccgggggaa
     1081 cgcgctgccg cggaggcgga actgcgcaag gcggaggcgg cgcatgactg gaacgccgcg
     1141 cgcgccgcgg ccgaacgccg catccggctg ggcaatgcgg gcgatcccgc gctatggctc
     1201 tcgctcgcgg ccgccgagct gaaggatccg accggatcgg ccaagaccgc gctgcaggcc
     1261 gcctatatcg gatttgccga acgccgcaac gccaccgatg aacccgcgct cacgcgccag
     1321 gcgctgctgc tgatgcggca ggcactgggc cgcctcggcg atcacctggc ggagatcagg
     1381 ttgctgggcg agatcgtgca ggcctggccg gacgatgcca cggcgaaagc gcagcttcag
     1441 cgcgccgtgt cgcgctacgg ctttgcggtg cgcaagatcg acaccgagcc gcagtctttc
     1501 ccggcgcggg cctgcatcgc cttcaccctg ccgctgccgg ccgacgaggc gctgcatccg
     1561 ggtgattatg tcagcaccga acccgcgatc gccgcgctcg cggtcagcgc ggagcagggg
     1621 cggctctgcc tgtccggcct gccgccgggc gccaccacgg tggtcaagct gcatcctggg
     1681 ctgccgggga tcgccggcac ccggctggag cgcatgctga ccatccggat cagcctggcc
     1741 aaccgccagc cgtcgctgct ggccgatccg acccacttca tcatcccggc cagccagccg
     1801 ccggcgatcg gttttgccag tgtcaacatc tccaagctga aggtgaagat cgcccgtgtg
     1861 gcggagcgca gcctgcgcgg ctttctgtcc aatcacccgc tgctgaatcc aggcgcctac
     1921 gccagcgggc tgaacggcga gaatgcaccc gtcgtgttca ccggcagtgc cggcgtgccg
     1981 gacttcgtac gcaaccggct gatgcacacc gtcctgccgc tggccgatgt gatgcagaag
     2041 ccgggtctgt acgcggtgtc gctggccccg ggcgacggca cgccggacga ggatggctcg
     2101 ctgaacctgg tgcaactggt gttgcgcacc aacctcgcgc cgaccacctg gcgcggggcg
     2161 gacgggctga ccgtgcagct acgccgcttc accgacgccg gcctcgtggc gggcgcgacg
     2221 gttgcgctga tcgcccgcga caacgccgtg ctggcgaccg cggacaccgg cgcggacggc
     2281 atggcgcatt tcgccgcgcc actgctggcc ggccccggcg gcgaggcgcc cgcggcgctg
     2341 cacatcaccg gcgccgatgg cgacttcacg ctggtgaacc tcgacgcctc gcccttcgac
     2401 ctgaccgacc gtggcgtgtc cggccgcccg cagccgaagc cgatcgaccc gtatatctgg
     2461 ctggaccgcg gcatctaccg tccgggcgag acgctgcatg tcggcgcgct gctgcgcagc
     2521 cccgacctca agccgctgga tgtgccgctg catctgatcg tgcggcgccc gggcgggcag
     2581 gtgttcgccg accaggtggt gcactggacc gctgattcgt cgctggtcga gccgatcacc
     2641 ctttcgccgg gcgcgcaggc cggcagctgg acgatcgcgc tggccatgtc cgacaaggac
     2701 ccggcgctgg ccagccgcag cttcaccgtc gccgctttcg tgccggctcg gctggcggtg
     2761 gacttcgcgc agcatccgcc gctggcgcct gggcgtatcg accaccagcc ggtgaccgtc
     2821 cgcttcctgt atggcgcgcc cggcgccaac ctgaccggca ccgccagcgt gcacatcacc
     2881 gccgatgcga cgcccttcgc cgccttcaag gactaccgct tcgggctgca cgacgagatc
     2941 accaatgcgc cgctgctgca gccgacgctg ccgcaaaccg atgccaaggg cgcgacctcg
     3001 gtgcccatcg acctgacgca tctgccggac gcgacgcatg cgctgaaggc gacggtcagc
     3061 gtcaccatca acgatccgtc cggacgcgcg gtgacgcgca gcacgacgct gccgatcgtg
     3121 ccgaaagcgg cgctgatcgg catcaagccg gacttcacgg gcggcgcggt ggaccagggc
     3181 gccaagcccg gcttcgagtt ggccgccgtc gcgccggacg gcaagccggt ggcgatgcag
     3241 gtggacctgt cgctggtgcg gcaggattac gagtgggagg tgatctggaa cgcctcggtg
     3301 gcgcgctggc acttcagcta catcgaccgg cccgtgctca cgcgcacgct gaccatcgag
     3361 ccgggccagc cctatcatct ggaactgccg gcgctgccct acgggcgcta ccggctgcgg
     3421 ttggtgcagc acgccggcgg gctggcggcc agttcggcca tcttctactc gggctgggtc
     3481 acctcggata atcccgcagt gccggcgcgg ctgaccgtgg cgcgcgacaa gcggatctac
     3541 gccgccggcg ataccgcgca cctgcacatc accgcgccct tcgccggccg cgccacgctg
     3601 gtgctggcca acagcaaggt gatcagcacg cgcgacttcg acctgtcgaa ggacggcgcc
     3661 gacctcacgg ttccggtttc cgccgattgg ggcgccggcg cctatgcggt ggtgcatgtc
     3721 taccggccag cggacggcaa gacgcccccc gaccgcgcgg ttggcctcac ctggcttggc
     3781 cttaccccgg gtgaccgcaa gctgccggtg acgatcgcgg cgcagaagct gtatcgcccg
     3841 gaccgtgatg tggacttcgc ggtgcacacc acgccgggcg cg
//
LOCUS       DASSZH010000008         2184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_324050, whole genome shotgun sequence.
ACCESSION   DASSZH010000008 DASSZH010000000
VERSION     DASSZH010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324050"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VFN42_00135"
     CDS             complement(<1..519)
                     /locus_tag="VFN42_00135"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET8995050.1"
                     /translation="MEPAHASSVYKEALIVLGAAGVVIPLVQRLRLSPVLGYMLVGMA
                     VGPFGLGALTARLPLLSAVTIADPTAIAPIAELGVVLLMFMIGLELSFERIWLMRRLV
                     FGLGSLQVALCAAALAGAGAALGRSGGEAAVIGIALAMSSTAVVLQVLSDEKRVNTPT
                     GRASFAVLLFQDL"
     gene            755..1012
                     /locus_tag="VFN42_00140"
     CDS             755..1012
                     /locus_tag="VFN42_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HET8995051.1"
                     /translation="MPKAGATVEGRRRLSGLVKRSFSLAGHRTSVALEEAFWTALLRV
                     AQERGQTLSALVAATDAARAPGQPLASALRVLALREAGATR"
     gene            1080..1259
                     /locus_tag="VFN42_00145"
     CDS             1080..1259
                     /locus_tag="VFN42_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995052.1"
                     /translation="MSHAITSPAASITQVSPLVLSDRLIALAQAADRAGYTETAEHLV
                     ELACSVFDEAPRPVN"
     gene            1352..>2184
                     /locus_tag="VFN42_00150"
     CDS             1352..>2184
                     /locus_tag="VFN42_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET8995053.1"
                     /translation="MASRDGAVARALATFDSGAFRQHLAELIAIPSTSQDPGHDADVD
                     RYLRQGIQPWLERMGFQVAIHPNPQPGFGPILTADRIEDPSRPTVLTYGHGDTVRGLE
                     DQWSAGLAPWTLTERGDRWYGRGTADNKGQHALNLTALEAVLAERGGKLGFNLKLVLE
                     TCEERGSIGLRDFVAAHRDMLAADVLIGSDGPRVAPDTPTVATGTRGTFHFDLVVALR
                     PGGVHSGHWGGLTTDPAVVLAHALACIADRKGRILVRDWLPANGLPAAVRSVLEGCPV
                     AG"
BASE COUNT          358 a          773 c          763 g          290 t
ORIGIN      
        1 caggtcctgg aacagcagca cggcaaagct ggcgcgcccg gtcggggtgt tcacccgctt
       61 ctcatccgac agcacctgca gcaccacggc ggtggacgac atggccaagg caatgccgat
      121 caccgcggct tccccaccgg agcgtccaag tgccgcaccg gcaccggcga gagcggcggc
      181 acacagcgcc acctgcagcg agcccaggcc gaacaccagg cggcgcatca gccagatgcg
      241 ctcgaaggac agctccagcc cgatcatgaa catcagcagc accacgccga gttcggcgat
      301 cggcgcgatc gcggtggggt cggcgatggt gacggcggac agcagcggca accgggcggt
      361 gagcgcgccc aggccgaacg gcccgaccgc catgcccacc agcatgtagc ccaacaccgg
      421 gctgagccgc aggcgctgca ccagggggat caccacgccg gcggcgccga ggacgatcag
      481 ggcttccttg tacaccgagg aggcatgggc cggttccatg tccggcggtt tagcaggtct
      541 gccgcgccgg gctaccgggg gataggcgac ggcccatcgc ggacatttcg gctcatcgcg
      601 caagctcgtt accgcactga atccgccgca tcggctatga cgggccagtg acgagtggca
      661 cggcggcgcc ggccggaaga ttcacgctgg cgcgcgtggc cgtgaaccgg cgcgagcggc
      721 cggggacaac gcatactggg cgatgcccag ggcgatgccc aaagcaggcg ccacggttga
      781 agggaggcgg cgtctgagcg gcctggtgaa gcgtagcttc tcgctggcgg ggcaccgcac
      841 cagcgtggcg ctggaagaag cgttctggac cgccctgctg cgcgtcgcgc aggagcgcgg
      901 ccagaccctg tcggcgctgg tcgccgccac cgacgcggcg cgcgcaccgg gccagccttt
      961 ggcctcggca ctgcgcgtcc tcgcgctgcg cgaggctggg gcaactcgtt aaccaatggg
     1021 aaagaactcg gcggcatact gccgcgtggc gcagaagccg ccttgtgaag ggaaaaccca
     1081 tgtctcatgc gatcaccagt ccggccgctt ccatcaccca ggtcagtccg ctggtgctgt
     1141 ccgaccggct gatcgccctg gcacaggccg ccgaccgcgc cggctacacc gagaccgccg
     1201 aacatctggt ggaactggcc tgcagcgtgt tcgacgaagc gccgcggccg gtgaattgac
     1261 ttggcagcct gccccgtccg gtgtgggagg ggagtagtcg cgctgcctcg cctgggttag
     1321 ctgttccgcc tccgacgcaa ggaacagcag gatggcatct cgtgacggcg cggtagcccg
     1381 cgccctggcg accttcgata gcggcgcatt ccgccagcat ctggcggaac tgatcgccat
     1441 tcccagcacc tcgcaggacc cggggcatga cgccgacgtg gaccgctacc tgcgccaggg
     1501 cattcagccc tggctggagc gcatgggctt ccaggtggca atccacccca acccgcagcc
     1561 gggctttggc ccgatcctca ccgcggaccg catcgaggat cccagccgcc cgaccgtgct
     1621 gacctacggg cacggcgaca cggtgcgcgg cctggaagac cagtggagcg ccggcctcgc
     1681 cccctggaca ctcaccgagc ggggcgaccg ctggtacggc cgcggcaccg ccgacaacaa
     1741 gggccagcac gcactgaacc tcaccgcgct ggaggccgtg ctggcggagc gcggcgggaa
     1801 attgggcttc aacctgaagc tggtgctgga gacctgcgag gaacgcggat ccatcgggct
     1861 gcgcgacttc gtcgccgcgc atcgcgacat gctggcggcc gatgtgctga tcggcagcga
     1921 cggcccacgc gtcgcgcccg atacgcccac cgtcgcgacc ggcacccgcg gcaccttcca
     1981 cttcgacctg gtggtggcgc tgcgccctgg cggcgtgcat tccggccact ggggcgggct
     2041 gaccaccgat ccggcggtgg tgctggcgca tgcgctggcc tgcatcgccg accgcaaggg
     2101 gcgcatcctg gtgcgcgact ggctgccggc aaacggcctg ccggctgccg tccgctccgt
     2161 gctggaaggc tgcccagtag ccgg
//
LOCUS       DASSZH010000009         3898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_903591, whole genome shotgun sequence.
ACCESSION   DASSZH010000009 DASSZH010000000
VERSION     DASSZH010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903591"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..977)
                     /locus_tag="VFN42_00155"
     CDS             complement(54..977)
                     /locus_tag="VFN42_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8995054.1"
                     /translation="MRMLRSPATWIVLLLLVVGITLPWWVSAYILGVLTVSYYFGVFA
                     MAWDLLFGFAGEVNFGPTFLIGLGAYTAGILDNALHPPIFVCVAAGAVVAMVGGALLA
                     LPALRLRGPYFGLVTLVAVLLLQNAIVIFAGVTGGEIGMNVSDVLSIDATTNYWIGLV
                     FMVVCGALLFGLSRSAIGLILQASGQDAIEAAALGFNVTKHKLAAFCVSALFSGLAGA
                     MLVFYEGTASVDTVVDIAIAVQIIIAAVLGGRRTILGAAIGAIILVLAGELLRPLGQL
                     NTFVVSAFALAVILFFPDGLLGHVLRRGERG"
     gene            complement(983..1858)
                     /locus_tag="VFN42_00160"
     CDS             complement(983..1858)
                     /locus_tag="VFN42_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8995055.1"
                     /translation="MQALQILIDGFAISALYALGAVGFTLMFGVCGVLNLAHGGIMVI
                     AALVGWLAAGTLGLGPYLGSMVGVIAALVAAYATYFVVVRPIQQSRAIPREETEIFVL
                     TGTLLWGIMIQVGMAWWFTDNPVTIRPLIGGVVNLAGVRTPTNELLIAALSWVAIGLL
                     WLLVNRTRAGKALLAASINPRGLVLLGFELDRIYLLVWTIYGVLAGIAGVLLASFLGV
                     GTNNVGQLTASAFSIVIMGGLGSVSGSLVAAYVVGYLETFTAYMIAPTLRPIPALILL
                     VLVVYVRPQGLLGRR"
     gene            complement(1942..3183)
                     /locus_tag="VFN42_00165"
     CDS             complement(1942..3183)
                     /locus_tag="VFN42_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8995056.1"
                     /translation="MSKPGWMRLSLLAGAAVLALAVTARAEEPIRIGVIGEESAVAGA
                     SITKAARMAADDINAKGGIGGRKVEVITYDDHSSAAEGVRAFQRAVSQDHVNAVIGSY
                     ISEVVLAMEPWSARLHMPFITPGAASNEISQQVHKDYAHYKYTFHGWLTSAFIAQSIC
                     DAEHDILVEPLHMKTAVIMSEDAAWTKPLDERFQECLPKAGLKVLDHIRFNPDTTDFT
                     PIFRQIEEKHPDVIITGISHVGVQPTAQWHDQQVPIPMAGQSSQATTSTFWKDTNGAA
                     NGVITASAATPGVALSPLTIPFTEAYQKRYGDSPSYSGYAAYDDVHIIAEAVQRAGST
                     DPDKIVEAMEKTDYVGTVGRIQFYGKDDPFTHALKYGPGLVTGVTFQWQDGKQVTLWP
                     ADKANGKVRFPSFVKLPQASN"
     gene            complement(3255..>3898)
                     /locus_tag="VFN42_00170"
     CDS             complement(3255..>3898)
                     /locus_tag="VFN42_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8995057.1"
                     /translation="PPEKAAAEEATGGSLAEIWVPPYRRRTIMLMVFNFCQSIGFYGF
                     GNWVPALLAAQGAGFTKSLEYSFIIALASPTLPFLFLLFADKTERKWQIVAAACGTAC
                     FGLLFTLSSQPAWLIFCGVAITASNNLLSYAFHAYQPELFPTRVRARAVGFVYSFSRL
                     STVFSSFMIAFFLARFGTPGVFGFIGVAMLIVVLSIGLYGPRTRGLALEEISR"
BASE COUNT          732 a         1331 c         1268 g          567 t
ORIGIN      
        1 gaccagcccg ccgaaccgct tgtgcagtcc tgccacttgc agaagcgggt cgctcatccg
       61 cgttctcccc gccgcaacac gtggccgagc aggccatccg ggaagaacag gatcacggcg
      121 agcgcgaagg ccgagacgac gaaggtgttg agctgcccga gcgggcgcag caattcgccc
      181 gccagcacca ggatgatcgc gccgatcgca gcccccagga tggtgcgccg cccgccaagc
      241 acggccgcga tgatgatctg caccgcgatc gcgatgtcga ccaccgtatc gaccgaggcg
      301 gtgccttcgt agaacaccag catcgcgccg gcgaggccgg agaacagcgc gctgacgcag
      361 aaggcggcaa gcttgtgctt ggtgacgttg aaaccgagcg ccgccgcctc gatcgcgtcc
      421 tgcccgctcg cctgcaggat caggccgatg gcggagcgcg acaggccgaa cagcagcgcg
      481 ccgcacacca ccatgaacac gagcccgatc cagtagttcg tggtggcgtc gatggagagc
      541 acgtcgctga cattcatgcc gatctcgccg ccggtgacgc cggcgaagat gacgatggcg
      601 ttctgcagca gcagcacggc aaccagcgtg accagcccga aatagggtcc gcgcagccgc
      661 agcgccggca gggccagcag cgcgccgccc accatcgcca ccaccgcccc cgccgcgacg
      721 cagacgaaga tcggcggatg cagcgcgttg tcgaggatgc cggcggtgta ggcgccgagg
      781 ccgatcagga aggtcgggcc gaaattcacc tcgcccgcga agccgaacag caggtcccag
      841 gccatcgcga acacgccgaa atagtaggag acggtgagca cgccgaggat ataggcggac
      901 acccaccagg gcagggtgat cccgaccaca agcagcagca gcacgatcca ggtggcgggg
      961 gagcggagca tgcgcatatc gcttatctcc gccccagcag gccttgcggc cgcacataca
     1021 ccaccagcac cagcaggatc agcgccggga tcggccgcag cgtcggcgcg atcatatagg
     1081 cggtgaacgt ctccagatac ccgacgacat aggccgccac cagcgagccg gagacgctgc
     1141 cgaggccgcc catgatgacg atggagaacg cgctcgccgt cagttgcccg acattgttgg
     1201 tgccgacgcc gaggaacgag gccagcagca cgccggcgat ccccgccagc acgccataga
     1261 tcgtccacac gagcagatag atgcggtcca gctcgaagcc gagcagcacg aggccgcgcg
     1321 gattgatcga ggccgccagc agcgccttgc ccgcccgcgt gcggttcacc agcagccaca
     1381 gcagcccgat cgccacccag ctcagcgccg cgatcagcaa ctcgttggtc ggcgtgcgca
     1441 cgccggcgag gttgacgacg ccaccgatca gcgggcggat cgtcaccgga ttgtcggtga
     1501 accaccacgc catgccgacc tggatcatga tgccccacag cagcgtgccg gtcagcacga
     1561 agatctccgt ctcctcgcgc gggatggcgc gtgactgctg gatcggccgc acgacgacga
     1621 aatacgtggc gtaggccgcg accaatgcgg cgatcacacc gaccatgctg ccgagatagg
     1681 ggccgaggcc aagcgttccg gcggcaagcc agccgaccag cgccgcgatc accatgatgc
     1741 cgccatgcgc caggttcagc acgccgcaga cgccgaacat cagcgtgaag ccaacggcgc
     1801 ccagcgcata gagcgcgctg atcgcgaatc cgtcgatcag gatttgcagt gcctgcatga
     1861 acctctcatc cgcgcgcatc ggcccgggcc acgccggccc gggccgatgc catcatgcca
     1921 gaggaccgtg tcgccgcaag ctcagttcga cgcctgcggc agcttgacga aggacgggaa
     1981 cctgaccttg ccgttcgcct tgtcggccgg ccacagcgtc acctgcttgc cgtcctgcca
     2041 ctggaacgtc accccggtga caaggcccgg cccgtatttc agcgcgtggg tgaacgggtc
     2101 gtccttgccg tagaactgga tgcgcccgac cgtgcccaca tagtcggtct tttccatcgc
     2161 ctccacgatc ttgtcggggt cggtggaacc cgcgcgctgc accgcctcgg cgatgatatg
     2221 cacgtcgtcg taggcggcgt agccggaata ggacggcgaa tcgccgtagc gcttctgata
     2281 cgcctccgtg aacgggatgg tcagcggcga cagcgcgacg ccgggggtgg cggcggaggc
     2341 ggtgatcacg ccgttcgccg caccgttcgt gtccttccag aacgtgctcg tcgtggcctg
     2401 ggagctttgc ccggccatcg ggatcggcac ctgctggtca tgccactgcg cggtcggttg
     2461 cacgccgaca tggctgatgc cggtgatgat cacgtccggg tgcttctcct cgatctggcg
     2521 gaagatcggg gtgaagtccg tcgtgtcggg attgaagcgg atatggtcca gcaccttcag
     2581 cccggccttc ggcaggcatt cctggaagcg ttcgtccagc ggcttcgtcc aggccgcgtc
     2641 ctcgctcatg atcaccgcgg tcttcatgtg cagcggctcg accaggatgt cgtgctcggc
     2701 atcgcagatc gactgcgcga tgaaggcgga ggtcagccag ccgtggaacg tgtatttgta
     2761 gtgcgcgtag tccttgtgga cctgctggga gatttcgttg ctggccgcgc cgggcgtgat
     2821 gaacggcatg tgcagccgcg ccgaccacgg ctccatcgcc agcaccacct cgctgatgta
     2881 gctgccgatc accgcattca catggtcctg gctgaccgca cgctggaagg cgcgcacgcc
     2941 ctcggcggcg gaggagtggt catcgtaggt gatgacctcg accttgcgcc cgccgatgcc
     3001 gcccttggcg ttgatgtcgt cggcggccat ccgcgccgcc ttggtgatcg aggcgccggc
     3061 caccgccgat tcctcgccga tcacgccgat ccggatcggc tcctccgccc gcgccgtcac
     3121 agcgagggcg agcacggcgg caccggccag cagcgacagg cgcatccatc ccggcttgga
     3181 catcgttcct cctcctcttt ccttcatgcc gccgcatcat gcacggatcg gccggcgtcg
     3241 ccaggggcgg cgcctcagcg cgaaatctcc tccagtgcca gcccgcgcgt gcgcgggccg
     3301 tacaggccga tcgacaacac cacgatcagc atcgccaccc caatgaaccc gaacacgccg
     3361 ggcgtgccga accgggccag gaagaaggca atcatgaaac tgctgaaaac cgtggagagc
     3421 cggctgaacg agtagacgaa tcccacagcc cgcgcccgca cccgtgtcgg gaacagctcc
     3481 ggctggtagg cgtggaacgc ataggacagc aggttgttcg acgcggtgat agccacgccg
     3541 cagaagatca gccaggccgg ctgcgacgac agggtgaaca gcaggccgaa gcaggcggtg
     3601 ccgcaggccg ccgcgacgat ctgccatttc cgttccgttt tgtccgcgaa caaaagaaac
     3661 aggaacggca gggtcggact cgcaagcgca atgatgaaag aatattccag actcttggtg
     3721 aacccggcac cttgcgcggc gagcagcgcc ggcacccagt tgccgaaccc gtagaacccg
     3781 atcgactggc agaaattgaa caccatcagc atgatggtgc ggcggcgata gggtggcacc
     3841 cagatctcgg cgagcgaccc gccggtggct tcctcggccg ccgctttctc gggcggcg
//
LOCUS       DASSZH010000010         4107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1363777, whole genome shotgun sequence.
ACCESSION   DASSZH010000010 DASSZH010000000
VERSION     DASSZH010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363777"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VFN42_00175"
     CDS             <1..339
                     /locus_tag="VFN42_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995058.1"
                     /translation="AAIRRQPVEAAIDAIRHDLGVCFSLCGARFGNALHDMAAAHRDT
                     LATVFHPFPQRETAVADAPARNATFALPPQKPDAIRYILGCLGAQQPVCGFPVMASPL
                     GVATAGAQPP"
     gene            597..1184
                     /locus_tag="VFN42_00180"
     CDS             597..1184
                     /locus_tag="VFN42_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HET8995059.1"
                     /translation="MRAKPQSDKPAPDEAALRDAALAHLARYAATETGLRRVLDRRIE
                     RWSRSATGGDPDAIAAQAASARQAARQVVARLAAAGAVNDAAFAESRARSLTRAGRSR
                     QAVAAHLAAKGVDRALALAATPDDPETQLAAALALARRRRIGPFRLGAEPDEAAKRRE
                     LGMLARAGFPQPVAQRALEMQPEEAEVLVAALRRG"
     gene            complement(1212..2603)
                     /locus_tag="VFN42_00185"
     CDS             complement(1212..2603)
                     /locus_tag="VFN42_00185"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET8995060.1"
                     /translation="MPDTDPALLTAEDMLALYARRVLSPVEVLQAVTERIARLNPQLN
                     AFAVLNPRALDAAAESEIRWRAGRPLGPLDGVPCTVKDLVDLAGFPTRRGSRTSDPAP
                     VADDAPLVVGLRSAGAVIVGKTTTTEYGWKSPGDCPLHGITRNPWNPAYTTGGSSSGA
                     GAAGAAGFGPLHLGTDAGGSIRIPAAWCGLVGLKPTYGRVPQWPASAFATVSCAGPMT
                     RSVRDAALMLTAMARYDLRDPFCLPDDGRDWRDGIEDGVSGLSVGVLRRPGFDAPVDS
                     DGVAAVERAAAILAEAGATVQDAQPDLPDTRLVFGRVWGAALARLVGTLPEQLRGLLD
                     PGILEVAQSQGGMTAIEFMDAEAMRAAAAHAMARLHQSFDLVLCPTVPAGPPLADAPT
                     TDPVQSLWTSWAPWTFAFNITRQPAITMPLGIGENGLPRSVQLAAAQHRDDLVLRAAR
                     VLELAEPVLVPEL"
     gene            2935..3180
                     /locus_tag="VFN42_00190"
     CDS             2935..3180
                     /locus_tag="VFN42_00190"
                     /inference="COORDINATES: protein motif:HMM:NF024861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HET8995061.1"
                     /translation="MSAVDVDGFLSAFASAAEPVELRYRWRPQLHDPADEMVLETAIN
                     GQAAALVTHNLRDLVAAGEHFGIAVIPPREALRRCCT"
     gene            3177..3428
                     /locus_tag="VFN42_00195"
     CDS             3177..3428
                     /locus_tag="VFN42_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HET8995062.1"
                     /translation="MSKGTYPLKLPQSIKRAAERLAKEDGVSLNQWISAAVAQKVGAV
                     ETAAELLRSRAGAARPEDLRKYLAAAPRVTPVPGDEIDH"
     gene            complement(3453..3713)
                     /locus_tag="VFN42_00200"
     CDS             complement(3453..3713)
                     /locus_tag="VFN42_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013635100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HET8995063.1"
                     /translation="MEQVAKLFKRGRSQAVRLPAAFRFDSNEVFIRQDPETGNVILSR
                     KPGSWDGFFAALQGSDIPAGFLQRAERHQPNAARDPFEGWRE"
     gene            3854..>4107
                     /locus_tag="VFN42_00205"
     CDS             3854..>4107
                     /locus_tag="VFN42_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HET8995064.1"
                     /translation="MTGSSKHPDGEPWQWPEATWRKIVGRARAGRSLRPASWPNNARC
                     AVAISFDADHETIPLRDNDESPMRISQGQYGNRQAMPRIRR"
BASE COUNT          658 a         1414 c         1446 g          589 t
ORIGIN      
        1 gcggcaatcc gccgccagcc ggtcgaggcc gccatcgacg ccatccgcca cgatctcggc
       61 gtgtgtttca gcctgtgcgg ggcgcgtttc ggcaacgcac tgcacgacat ggccgcggcg
      121 catcgcgaca cgcttgccac cgtctttcat ccattccccc agcgcgaaac ggccgtcgcc
      181 gacgcgccgg cgcggaatgc caccttcgcc ttgccaccac agaaaccgga tgccattcgc
      241 tacatcctgg gctgccttgg cgcgcagcag cccgtctgtg gtttccccgt catggcctcg
      301 ccgctcggcg tggcaaccgc gggagcccag ccaccctgaa cctgcctgat gcatcgcatt
      361 tgtccgctgc acgatcgggg ctgcaaacca gccagcgccg ccgcgcgtac gcgtgccgcg
      421 gcaggatctt cgcatcccat tttgtcacgt gcagcgcagg cccgccgcac gacacaccgt
      481 gtgcctgaat cgccgccatg cgactcgccg tcctggtttg ctggcgacat ccaacgcgtt
      541 agaagcccct ggccgggcac gatctgccat ccggcgattc gatcagcgag cgggcgatgc
      601 gtgcaaagcc gcaatccgac aaaccagctc ccgacgaagc cgcgctgcgc gacgcagcgc
      661 tggcgcatct ggcgcgctac gctgcgaccg agaccgggct gcggcgcgta ctggaccggc
      721 gcatcgagcg ctggtcacgg tccgcgacgg gcggcgatcc cgacgccatc gccgcgcagg
      781 cagcgtcagc gcggcaggcc gcgcgccagg tcgtcgctcg gctggcggcg gcgggcgcgg
      841 tgaacgatgc ggcttttgcc gagagcagag cgcgcagcct gacccgcgcc ggccgctccc
      901 gccaggcggt ggcggcgcat ctggcggcca agggcgtgga tcgggcgctg gccctggcgg
      961 ccacgccgga cgatcccgag acgcagctcg ccgcggcact ggccctggcg cggcggcggc
     1021 ggattggccc gttccgtctg ggcgccgaac ccgacgaggc cgcgaagcgg cgcgagctag
     1081 ggatgctggc gcgggccggg tttccgcagc cggtcgcaca gcgtgcgctg gaaatgcagc
     1141 cagaggaggc ggaagtactg gtggcggcgc tgcggcgcgg gtgaccgcgg ccaccctgcc
     1201 gccttcgaac atcagagttc gggcaccagc accggttcgg ccagttccaa cacccttgcg
     1261 gcgcgcagca ccagatcgtc acgatgctgc gctgccgcga gctgcaccga gcgcggcagg
     1321 ccattctccc cgatgcccag cggcatggtg atcgccggct gccgggtgat gttgaaggcg
     1381 aaggtccagg gcgcccagct cgtccacagc gactgcaccg ggtccgtggt gggcgcgtcg
     1441 gccagcggtg ggccggcggg cacggtggga cacagcacca ggtcgaagct ctggtgcagc
     1501 cgcgccatgg catgcgccgc ggcggcgcgc atggcctcgg catccatgaa ctcgatcgcc
     1561 gtcatgccgc cctggctctg cgccacctcc aggatgccgg gatcgagcag gccgcgcagt
     1621 tgctccggca gtgtgccgac caggcgggcg agcgcggcgc cccacacccg gccgaacacc
     1681 agccgcgtat ccggcaggtc cggttgggcg tcctgcacgg tggcgccagc ttctgccagg
     1741 atcgcggcgg cacgttccac cgcggcgacg ccgtcgctgt ccacgggagc gtcgaagccg
     1801 gggcggcgca gcacgccgac ggacaggccg gagacgccgt cctcgatgcc gtcgcgccag
     1861 tcgcggccgt catccggcag acagaacggg tcgcgcaggt cgtagcgcgc catcgcggtc
     1921 agcatcagcg cggcgtcgcg cacgctacgg gtcatcggcc ccgcgcagga gaccgtggcg
     1981 aaggcgctgg ccggccattg cggcacgcgg ccataggtcg gcttcaaccc gaccaggccg
     2041 caccaggccg cggggatgcg gatcgagccg ccggcatcgg tgcccaggtg cagcggcccg
     2101 aatccggccg ctcccgccgc gccggcgccg gatgaggagc cgccggtggt gtaggccggg
     2161 ttccagggat tgcgggtgat gccgtgcagc gggcaatcac cgggcgactt ccagccgtat
     2221 tcggtggtgg tggtcttgcc gacgatcacc gcgccggcgc tgcgcagccc caccaccagc
     2281 ggcgcatcat cggccaccgg cgccgggtct gaggtgcggc tgccgcggcg ggtgggaaag
     2341 ccggccaggt ccaccaggtc cttcaccgtg cagggcacac cgtccagcgg cccgagcggc
     2401 cggccggcac gccagcggat ctcgctctca gcggcggcgt ccagggcgcg cgggttgagc
     2461 accgcgaagg cgttgagctg gggattcagc cgagcgatgc gctccgtgac ggcttgcagc
     2521 acctccaccg ggctgagcac ccgccgggcg tacaacgcga gcatgtcctc ggcggtgagc
     2581 agagcgggat cggtgtctgg catgcggcac actcctgcgg cgggtggacg cagcatggac
     2641 gcggcggagc ggcgagggaa cccgtttggt gtgacggatg ccgggagggg ggccacccgg
     2701 gcgccagtgc cggcactctt cttgacatcc catgggaggt catccagcta gcgtcatgtg
     2761 gcgggttgta ctggacacgt cagttctggt cgcggggctc cgcagcagcc tgggcgccag
     2821 ttctcggttg ctgggtcttg ttgcggacgg cgtcctcagg ccgctggtca gcacaccgct
     2881 gtttcttgag tatgaggatg tgctgaaacg aacggaccac ctgcacgcga tgggatgagc
     2941 gcagtcgatg tggacggctt cttgtcggca ttcgcgagcg ctgcggagcc ggtggaactg
     3001 cgctaccgtt ggcggccgca gttgcatgat cccgcggacg agatggtgct tgaaaccgca
     3061 atcaatgggc aggccgccgc actggtgacg cacaatctgc gtgacctcgt cgccgcgggc
     3121 gagcatttcg gcatcgccgt catcccgccg cgcgaagcat tgaggagatg ctgcacatga
     3181 gcaagggcac gtatccactg aagctaccgc aatcgatcaa gcgtgctgcc gagcggctgg
     3241 caaaggaaga cggtgtctcg ttgaatcagt ggatttccgc agcagtggcg cagaaagtcg
     3301 gggcggtaga aacggcggcg gaactgctgc gcagccgggc tggagcagcg cggccagagg
     3361 atctcaggaa atacctcgcg gcagcgcctc gggtcacgcc ggtgcccggc gacgaaatcg
     3421 accattgagc cgcatgttga gcacgtggag cgtcattcgc gccatccctc aaaaggatcg
     3481 cgcgccgcat ttggctgatg gcgctccgca cgttggagga agccggcggg aatatcgcta
     3541 ccctgcagtg cggcgaaaaa accgtcccag ctccccggct tgcgggacag aatgacgttg
     3601 ccggtttccg ggtcctgccg aatgaacact tcgttgctgt cgaaacggaa tgccgccggc
     3661 agccggacag cctggctgcg gccccttttg aacagtttgg caacttgttc cactgcgttc
     3721 ccccgtgatc aacctgccga agctgggaga gcagcagaga tatatccaga tctattccga
     3781 ggatacccac tgcattccca accgccgcga acgcgcgcag catgccccgg gaccagacag
     3841 acggaggcgt cgcatgaccg gcagcagcaa gcaccccgac ggcgaaccct ggcagtggcc
     3901 ggaggcgacg tggcgcaaaa tcgtcggccg cgcccgggcc gggcgcagcc tgcggccggc
     3961 atcctggcca aacaacgccc gctgcgcggt ggcgatcagc ttcgacgccg accacgaaac
     4021 cattccgctg cgcgacaatg acgaaagccc gatgcgcatc agccagggcc agtacggcaa
     4081 ccgccaggcc atgccgcgta tccgccg
//
LOCUS       DASSZH010000011         5765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1397891, whole genome shotgun sequence.
ACCESSION   DASSZH010000011 DASSZH010000000
VERSION     DASSZH010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397891"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..1333)
                     /locus_tag="VFN42_00210"
     CDS             complement(23..1333)
                     /locus_tag="VFN42_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8995065.1"
                     /translation="MDGSYAAAEPPAVGKSRWFRLIPIAMAIYIISFMDRTNIGYAFA
                     GIGHDFHVGKAEQGAAGGIFFIGYVLLQIPGGWAAEHWSAKKFIAVMIAFWGAMAILC
                     GAAANFEQLMIFRFLLGVAEGGVWPAMLVLISHWFPVRERARAYSFWMANLAISSIIT
                     QPLSGWIVSATDWRALFYIEGALPFIIALPLWLLFVKDRPSQASWCSPEERKYIETSL
                     ARDNAGQPEQASFWTIFQSGTVWQLVAVYFLVQVGFYGLNMWMPTLLKHLTSAGFGAV
                     GLIATLPYFTAIVLMWFNGVYADRSLKYSRHVLLPMVLASVSLVASVLIGNLVLALSV
                     FFLCLAMGGALSYDGPFWAAGSRALPPVVVGGAMGLINALGNLGGYLGPFVGGWLQQV
                     TGGFVWTAVFLAGSLLLAGLVMMTVHVRAVPRPVSAADLAQRHI"
     gene            complement(1698..2405)
                     /locus_tag="VFN42_00215"
     CDS             complement(1698..2405)
                     /locus_tag="VFN42_00215"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HET8995066.1"
                     /translation="MIAFDVRLHERIGSTNDEARRLALEGAASGTVVCADEQTTGRGR
                     QARHWFSPPGNLYFSLLLRPDVPSARLPELAFVSALAVADTADALLPPSIRASLKWPN
                     DVLVKGAKVAGILLEQVGDALVVGVGLNILHAPERASYQVTTIAACGGLATVDGARAI
                     LLDRMARQLQTWEEDGFAPIRAAWLVRAHPVGAALRVTLEGRSLSGHFAGLDQDGALL
                     LDMPEGRRRVVAGDVVV"
     gene            2834..4555
                     /locus_tag="VFN42_00220"
     CDS             2834..4555
                     /locus_tag="VFN42_00220"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HET8995067.1"
                     /translation="MSDESDTPDPAPEHAAHPQAVHQQSRWPGWIWSIPIAAVAIVGY
                     LGFQQLARSGPTVTVIFPIQGGIKANQTKVEYRGADVGEVSTVTFEKDLKHMKVKLQM
                     GPDMAGHLGKGTRFWIAGHPNITDLASVKSVITGPHIGVEPQPGSAQDHYIGLGRRPV
                     NAYNTHGRRYLLQADELGTITRGSVVSYRGMKVGEVEDTTLEKDHKHFRIAVFVNAPY
                     DAMVHQGTRFWNASAVQVALTGGGPHLQFQSLPALFQGAIGFETPDQAAGPVARPDTT
                     FRLYKSKSTAEHASGAEAVAYRAVFHAADAGGLKAGSPVMLMHKQVGTVTASTLQYDP
                     RQKQLEEVVTLGIEPWRIGLAGGTHWAANPRPQMNALIQQLIAQGLRARLGSTIPLVG
                     GKTIDLAFVSGAKPASLGSGNPPELPTGPESGIGGVLATVNNVADTINGIAAKLNALP
                     LDQIADNIRTVTQRLAGLSKSPELTQTLQNLDKSMANVEQVTSEAKGQIGPILQALHR
                     AAHEADATVAAAHGLVSSNAFTRNAPGTASIGSTLYEVKEAARSLRALANYLDAHPAA
                     LLHGRGG"
     gene            4558..5130
                     /locus_tag="VFN42_00225"
     CDS             4558..5130
                     /locus_tag="VFN42_00225"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HET8995068.1"
                     /translation="MKWYVLAVATPLLLAGCGSSPPTHFFTLNPVKPAAGTPPTHAKG
                     PPLQVRDVQLPPTLDRLEMVLRGPGTQVQVLGSDRWAAPLKGLIRQTLTQDLRDRLGE
                     NAVEATGTPTQPGKVQVLIVTVQEFSADASGRVTLDANWALGRGNPPKPVLNRHATVH
                     ADAGSVKPGAVADAMSRALGALADRIAAAL"
     gene            5306..>5765
                     /locus_tag="VFN42_00230"
     CDS             5306..>5765
                     /locus_tag="VFN42_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8995069.1"
                     /translation="MADHDLQGMINRVKQGRLSRRRFIERMAVLGLTAPMAAQLLSMG
                     GVAIAAPANNGYKPTKRGGGGTLKLLWWQAPTLINPHFATGTKDQDASYLFYEPLAVW
                     DADANLVLKLGAEVPTLQNGGLSKDGKTVVWKLKQGVKWHDGKPFTADDVV"
BASE COUNT         1080 a         1957 c         1901 g          827 t
ORIGIN      
        1 cgcgcctgtt gcaggcgtgc cgtcagatat gccgctgcgc cagatcggcc gccgaaacgg
       61 ggcgtggcac cgcgcgcacg tgcacggtca tcatcaccag cccggccagc agcagggatc
      121 ccgccaggaa caccgcggtc cacacgaacc cgcccgtcac ctgttgcagc cagccaccga
      181 cgaagggccc gagatagccg cccaggttgc ccagcgcgtt gatcagcccc atcgcgccgc
      241 cgaccaccac gggcggcagc gcacgcgagc ccgctgccca gaacggtccg tcgtaggaca
      301 gcgccccacc catcgcgagg cacaggaaga acaccgacag cgccagcacg aggttgccga
      361 tcagcaccga cgccaccagg ctgaccgagg ccagcaccat cggcagcagc acgtggcgcg
      421 aatacttcag gctgcggtcg gcgtacacgc cgttgaacca catcagcacg atcgcggtga
      481 aatacggcag cgtcgcgatc agccccaccg cgccgaaccc ggcggaggtc aggtgcttca
      541 gcagcgtcgg catccacatg ttcaggccgt agaagccgac ctgcaccagg aaatacaccg
      601 ccaccagctg ccacaccgtg ccagactgga agatcgtcca gaaggacgcc tgctccggct
      661 ggcccgcatt gtcgcgcgcc agcgatgttt cgatgtactt gcgctcctcc ggcgaacacc
      721 aggatgcctg gctgggacgg tccttcacga acagcagcca cagcggcagc gcgatgataa
      781 agggcagtgc gccttcgatg tagaacaagg cacgccagtc ggtggcgctg acgatccagc
      841 cggacagcgg ctgggtgatg atcgacgaga tcgccagatt ggccatccag aagctgtagg
      901 cccgggcacg ttcgcgtacc gggaaccaat ggctgatcaa caccagcatg gcgggccaca
      961 caccgccttc ggcgacgccg agcaggaagc ggaagatcat gagctgctca aaattggcgg
     1021 cggccccgca caatatcgcc atcgcgcccc agaacgcgat catcaccgcg atgaacttct
     1081 tcgcgctcca gtgttccgcc gcccagccgc cgggaatttg cagcagcacg tagccaataa
     1141 agaagatgcc gccggccgcg ccctgctcgg cctttcccac atggaaatcg tggccgatgc
     1201 ccgcgaaggc gtagccgata ttggtccggt ccatgaagga gatgatgtag atcgccatgg
     1261 cgatcgggat gagccggaac cagcgtgact tgccgaccgc cggcggttcg gccgccgcgt
     1321 acgacccgtc catgggcgct actcctgaaa accggcgcaa ggcgaaattg ctgcgcgaga
     1381 gcccggaaac gcggaagtgg cggtcacggt tgagggagct tccccctgcc ctgttcatgc
     1441 gggcgtgggc gaagcggagg tgccgcgtcg tggcgggcga agatgtccct gccaaacagc
     1501 gaccagtctt ggcgggcgaa ggcctgccat tcatgactgg ctggaggaaa ctcccaaagc
     1561 ggtttgcagc tcccgagtct ccccaacaaa cagtcggtag caggccttcg cctgccacga
     1621 cgatgggcgc atcgccgaac aaatgtcagc gcttatggac ttcgcccgcc gcgccgggga
     1681 ccgctacgcc cggtgagtca gaccaccacg tcgcctgcca cgacgcgccg gcggccttcg
     1741 ggcatgtcca gcagcaaggc cccgtcctga tccagcccgg cgaagtggcc cgacagcgaa
     1801 cggccttcca gcgtcacccg caatgccgcg ccgaccggat gcgcgcgcac cagccaggcg
     1861 gcgcggatcg gcgcgaagcc gtcctcctcc caggtctgaa gctgccgcgc catgcggtcg
     1921 agcaggatgg cccgcgcgcc gtccaccgtc gccagtccgc cgcaggcggc gatcgtggtc
     1981 acctggtagc tggcccgttc cggcgcatgc aggatgttga gcccgacgcc aaccaccagc
     2041 gcatcgccta cctgctccag caggatgccc gcgaccttcg cccccttcac cagcacgtcg
     2101 ttcggccatt tcaagctggc gcggatggat ggcggcagca gcgcgtcggc ggtatccgcc
     2161 accgcgagtg cgctgacgaa agccagctcg ggcagccgag cgctcggcac gtccggccgc
     2221 agcagcaggg agaaatacag attgccgggc gggctgaacc aatgccgcgc ctggcgtccc
     2281 cgcccggtgg tctgctcatc ggcgcacaca accgtgcccg aagcggcgcc ttccagcgcg
     2341 aggcggcgtg cctcgtcatt ggtggagccg atgcgctcat gcagccggac atcgaaggcg
     2401 atcatgccgg cacgccgggc gtgttgcagg acgggcgcgg acaaaggcgg tgtggcaagg
     2461 ggcacatgac gccgcagctt agcgctgatc gcggtgcttg tgggaggggc ggccgccacg
     2521 cccgcaccac gcggcgggat tgacgccggt gtgatccggc acgcgccgct cttgctgccg
     2581 ccgaaagagt tgaaagtccc gcgcggcgcc gcaaagcgaa acggcaaacc tggaacgccc
     2641 ggttgcgttg ctggcacgag cgcgtgcgcc gaaccgccca gacgcaggag accgtgattg
     2701 cttataccga tggcggagcc gtccgcccca gttcagcgcc gcttccgtgc tgccgagcag
     2761 cctgccggtg atcgggccgc atgcaaactc tgctgatctt gattgaagga acgcgttctc
     2821 cgggatcgat gggatgagcg acgaatccga cacgcccgat cccgcgccgg agcatgcggc
     2881 gcatccgcag gccgtgcatc agcaaagccg ttggcccggc tggatctggt cgatcccgat
     2941 cgcggcggtg gcgatcgtgg gctatctcgg cttccagcag ctcgcccgca gcggcccgac
     3001 cgtgacagtg atcttcccca tccagggcgg catcaaggcc aaccagacca aagtggaata
     3061 ccggggcgcc gatgtcggcg aagtctccac cgtcaccttc gagaaggatc tgaagcacat
     3121 gaaggtgaag cttcagatgg gtcccgacat ggcgggtcat ctggggaagg gcacgcgctt
     3181 ctggatcgcc ggtcatccga acatcaccga tcttgcgtcg gtcaaatccg tgatcaccgg
     3241 accgcatatc ggcgtggagc cgcagcccgg ttccgcgcag gaccactata tcggcctcgg
     3301 ccgccgtccg gtgaacgcct ataacaccca cggtcgccgc tatctgcttc aggccgatga
     3361 actcggcacc atcacccgcg gctcggtcgt cagttaccgc ggcatgaaag tcggcgaggt
     3421 ggaagacacc acactggaaa aggaccacaa gcacttccgc atcgcggtgt tcgtcaacgc
     3481 gccgtatgac gcgatggtac accagggcac ccgcttctgg aacgccagcg cggtgcaggt
     3541 ggcgctgacc ggcggcggcc cgcatctgca attccaatcg ctgccggcct tgttccaggg
     3601 cgccatcggc ttcgaaacgc cggaccaggc cgccggtccg gttgccaggc cggacacgac
     3661 attccgcctg tacaaatcca agagcaccgc ggaacatgca tcgggagccg aggccgtcgc
     3721 ctaccgcgcg gtgtttcacg ccgccgatgc gggcgggctg aaagccggct cgccggttat
     3781 gctgatgcac aaacaggtcg gcacggttac cgcgagtacg ctgcaatacg acccgcggca
     3841 gaagcagctt gaggaagtgg tgacgctggg gattgagccc tggcgtatcg gcctggccgg
     3901 cggcacgcat tgggcagcca atccgcgccc gcagatgaac gcgctgatcc agcagctgat
     3961 cgcccagggt ctgcgtgccc ggcttggcag caccatcccg ctggtcggcg gcaagaccat
     4021 cgatctcgcc ttcgtctccg gcgccaagcc ggccagcctg ggcagcggca atccgcccga
     4081 actgcccacc ggcccggaat ccggcatcgg cggcgtgctg gccacggtga acaacgtcgc
     4141 cgataccatc aacggcatcg cggcaaagct gaacgcgctg ccgctggacc agatcgccga
     4201 caacatccgc acggtgacac agcgcctcgc cgggctgagc aagtcgcccg aactgacaca
     4261 gacgctgcag aacctcgaca agtcgatggc caatgtcgag caggtgacca gcgaagccaa
     4321 gggacagatt ggcccgatcc tgcaggcgct gcatcgcgcc gcgcatgaag ccgacgccac
     4381 ggtcgccgcg gcgcacggcc tggtcagctc caatgcgttc acccgcaacg cgccgggcac
     4441 cgcgagcatc ggctcgacgc tgtacgaagt gaaggaggcc gcccgcagcc tgcgtgcgct
     4501 ggccaattac ctggatgcgc atccggcggc gctgctgcat ggaagaggag gctaggcatg
     4561 aagtggtacg tgctggcggt tgcgacgccg ctgctgctgg cgggatgcgg ctccagcccg
     4621 ccgacgcact tcttcacgct gaacccggtg aagcccgccg ccggcacgcc gcccacgcat
     4681 gccaaggggc cgccgctgca ggtgcgcgat gtgcagttgc cgcccacgct cgatcggctg
     4741 gagatggtgc tgcgtggccc cggcacccag gtgcaggtgc tgggcagcga ccgctgggcc
     4801 gcgcccttga aggggctgat ccgccagacc ctgacgcagg atctgcgcga tcggctgggg
     4861 gagaacgcgg tggaggcaac cggcacgccg acgcagcccg gcaaggtcca ggtgctgatc
     4921 gtgaccgtgc aggagttcag cgccgatgcg tccggccgcg tgacgctgga tgccaactgg
     4981 gcactgggac ggggcaatcc gccgaagccg gtgctgaacc gccacgccac ggttcacgcc
     5041 gatgccgggt cggtcaaacc gggagcggtt gccgacgcga tgagccgagc gcttggcgcg
     5101 ctggcggatc ggatcgcagc ggcgctgtag cgtccgattg cccgaattcc gggcaattcc
     5161 ggctttgtca atttcgccgg aacacgaaat gtgcacatga ggcttgaccg caaagcacag
     5221 gtctgatgct gttgctgccg cagggacttc acgaagcgca cgctcggaac cgggcgcgcg
     5281 ccaattccgg gtgcgggaga cagcaatggc agaccacgac cttcaaggta tgatcaaccg
     5341 ggtgaagcag ggacgcttgt cccgacgacg cttcatcgag cgcatggcgg tgcttggtct
     5401 caccgcgcca atggcggcgc aattgctgtc gatgggcggg gtggccatcg cggcaccggc
     5461 caacaacggc tacaagccca ccaagcgcgg cggtggcggc acgctgaagc tgctgtggtg
     5521 gcaggcgccg acgctgatca atccgcactt cgcgaccggc accaaggacc aggacgcatc
     5581 gtacctgttc tatgagccgc tcgcggtgtg ggacgcggat gccaacctgg tgctgaagct
     5641 cggcgccgag gttcccacct tgcagaacgg cggcctgtcc aaggatggca agaccgtcgt
     5701 ctggaagctg aagcagggcg tgaagtggca cgacggcaag cccttcaccg ccgacgacgt
     5761 ggtgt
//
LOCUS       DASSZH010000012         2946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_545660, whole genome shotgun sequence.
ACCESSION   DASSZH010000012 DASSZH010000000
VERSION     DASSZH010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545660"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2207)
                     /locus_tag="VFN42_00235"
     CDS             complement(<1..2207)
                     /locus_tag="VFN42_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HET8995070.1"
                     /translation="MDVGLARAPMTLDSRFVEHDQPVLLTGIQALVRLLLEQADLDRA
                     AGLHTAGLASGYRGSPLGGLDHELWRQQRKLAEHDIRFEPGVNEDLAASMLFGTQQLD
                     VFPGAKFDGVFGLWYAKGPGVDRSGDALHCANMTGTHRNGGVVAVAGDDHGAHSSTYP
                     HQTEFMFQNVFMPVLNPANAREILELGLAGYALSRFCGLWIALKTTAETVEQAATFSI
                     RSRRRFIVPDFPIPPHGLNLDPKLRFPADRIAMEQRVVDERLPAALAWARANNLDRLV
                     FGRADATVGLITVGKAHEDTLHALTLLGLRDHPQIALYKVAMTWPLETEGLRAFARDK
                     RALFVIEEKRPFVEAQLRDALYHLPADSRPDIIGKTDARGAPLLSPLMELSPESVAAA
                     MARLLPALGINTPAPESLPAIDRPDGMPRRTPAFCAGCPHQTSTRVPEGSFATGGIGC
                     HIMALDEGDQTRSFIQMGAEGVPFVGLQPFTETKHTFANLGDGTYQHSGILAIRQALA
                     AHARITYKLLFNDAVAMTGGQPAEGAPTVRRIAAQLAAEGVQRIAVVADAADRLPRAK
                     DLPPGTTRHTREQLDAVQRELREYDGVSVLIYDQVCATEKRRRRKRGSMAQPARHVVI
                     NQAVCENCGDCTTQSGCIAIEPVETELGRKRRINPTSCNVDLSCLKGFCPSFVTVAGP
                     PQAPAADPSWQAREDALARDLPLPVLPSGSVWRGLFAGIGGGGIVTAGAVLAMA"
     gene            2365..2832
                     /locus_tag="VFN42_00240"
     CDS             2365..2832
                     /locus_tag="VFN42_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HET8995071.1"
                     /translation="MDLDRLDVAILDYLQAHGRATTTELSEAVALSPSPIHRRQKMLE
                     EAGVIQRYVAVLDQEKVGLSVNAFVTVKLATHTDDALAAFERAIEACSEVMELYEMTG
                     ARDYLRRVVAPDLTSFESFLRHVVMRIPGVQSVETAMALKRVVYRTSLPLRAR"
BASE COUNT          447 a          965 c         1066 g          468 t
ORIGIN      
        1 gccatggcca gcaccgcgcc ggcggtgacg atgccgccac cgccgatgcc ggcgaacagc
       61 ccgcgccaca ccgaaccgct cggcagcacc ggcagcggca ggtcgcgcgc cagcgcgtcc
      121 tcccgtgcct gccacgacgg gtcggcggcg ggcgcttgcg gcgggccggc gacggtgacg
      181 aagctcgggc agaagccttt caggcaggac agatcgacgt tgcagctggt cgggttgatg
      241 cgccgcttgc ggccgagctc ggtttccacc ggctcgatgg cgatgcagcc ggactgcgtg
      301 gtgcagtcgc cgcagttctc gcacaccgct tgattgatca ccacgtggcg ggccggctgc
      361 gccatgctgc cgcgcttgcg gcggcggcgc ttttccgtgg cgcagacctg gtcgtagatc
      421 agcacggaga cgccgtcata ctcgcgcaat tcgcgttgca cggcgtcgag ctgctcgcgc
      481 gtgtgccgcg tggtgcccgg cggcagatcc ttcgcgcgcg gcaggcgatc ggcggcgtcg
      541 gccaccacgg cgatgcgctg cacgccctcc gccgcgagct gcgcggcgat gcgccgcacg
      601 gtcggcgcgc cttccgccgg ctggccgccg gtcatcgcca ccgcgtcgtt gaacagcagc
      661 ttgtaggtaa tgcgcgcgtg cgcggcgagc gcctggcgga tcgccaggat gccggagtgc
      721 tgatacgtgc cgtcgcccag attggcgaaa gtgtgcttcg tctcggtgaa gggttgcagg
      781 ccgacgaagg gcacgccttc cgcgcccatc tggatgaagc tgcgggtctg gtcgccttcg
      841 tccagcgcca tgatgtggca gccgatgccg ccggtggcga agctgccctc cggcacgcgg
      901 gtggaggtct ggtgcgggca gccggcgcag aaggccggcg tgcggcgcgg catgccatcg
      961 gggcggtcga tggccggcag cgattccggt gccggcgtgt tgatgcccag cgccggcagc
     1021 agcctggcca tcgccgcggc gacggattcg ggggaaagtt ccatcagcgg cgacagcagc
     1081 ggcgcgccgc gcgcgtcggt tttgccgatg atgtcggggc ggctgtcggc cggcaggtga
     1141 tacagcgcgt cgcgcagttg cgcttcgacg aagggccgct tctcctcgat gacgaacagc
     1201 gcgcgcttgt cgcgggcgaa ggcgcgcagg ccttcggttt ccagcggcca ggtcatcgcc
     1261 accttgtaga gcgcgatctg cggatggtcg cgcaggccga gcagggtgag ggcgtgcagc
     1321 gtgtcctcgt gcgccttgcc gacggtgatc agcccgacgg tcgcgtcggc gcggccgaac
     1381 accagccggt ccagattgtt ggcgcgcgcc caggcgagcg cggcgggcag gcgctcgtcc
     1441 accacgcgct gttccatggc gatacggtcg gcggggaagc gcagcttcgg gtccaggttc
     1501 agcccgtgcg gcgggatcgg gaaatcgggg acgatgaagc ggcggcggga gcggatgctg
     1561 aaggtggcgg cctgttccac cgtctccgcg gtggtcttca gcgcgatcca cagcccgcaa
     1621 aaacgcgaca gcgcataccc ggccaagccg agttccagga tctcccgcgc gttcgccgga
     1681 ttcagcaccg gcatgaacac gttctggaac atgaattcgg tctggtgcgg ataggtggag
     1741 gaatgcgcgc catgatcgtc gcctgccacc gccaccacgc cgccgttgcg atgcgtgccg
     1801 gtcatgttgg cgcagtgcag cgcgtcgccg gagcgatcga cgccggggcc tttcgcgtac
     1861 cacaggccga acacgccatc gaacttcgcg ccggggaaca catcaagctg ttgtgtgccg
     1921 aacagcatgc tggcggcgag gtcttcgttc acccccggct cgaagcggat gtcatgctcc
     1981 gccagcttgc gctgttgccg ccacaactca tggtccaacc cgcccagcgg cgaaccacga
     2041 tagccggagg cgaggcccgc ggtgtgcaat cccgcggcgc ggtccaggtc cgcctgttcc
     2101 agcagaagac gcacgagtgc ctggatgccg gtcagcaaca ccggctggtc gtgctcaacg
     2161 aaacgcgagt ccagcgtcat cggcgcgcgc gcaaggccga catccatggc gatactcctc
     2221 cgtggcagtg gcttgtgccg cctccgcccc catggatcgg actctaggtg ggcgcgcgcg
     2281 ggaaaggctg ccatagttgc gcggggacgc acgccgattt ggcagagtct gccaaattgc
     2341 tgcgctgcaa ccaggaaagt gcggatggac ctcgaccggc tggatgtcgc gattctggac
     2401 tacctgcagg ctcacggccg cgcgacaacg acagagctga gtgaagcggt ggcgctgtcg
     2461 ccgtcgccga ttcaccgccg gcagaagatg ctggaggaag ccggcgtcat ccagcgctat
     2521 gtcgcggtgc tggaccagga gaaggtcggg ctgtccgtca acgccttcgt gacggtgaag
     2581 ctggcgacgc acaccgacga cgcgctggct gccttcgaac gggcgatcga agcgtgttcc
     2641 gaagtgatgg agctgtacga gatgaccggg gcgcgcgact acctgaggcg ggtggtggcg
     2701 ccggatctga cgtcattcga gtcgttcctg cgccacgtgg tgatgcgcat tcccggcgtg
     2761 cagtcggtgg aaaccgcgat ggcgctgaag cgggtggtgt atcgcaccag cctgccgctg
     2821 cgcgcgcgtt gaacacgcgg tcctgggccg cgaattcttg catggtctgc gcgacctcgc
     2881 gtccggtgca ccattaaccc gcacgcgttc ccacggaccg gttccgaacg gccgagcgcg
     2941 aaagct
//
LOCUS       DASSZH010000013         2137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_34315, whole genome shotgun sequence.
ACCESSION   DASSZH010000013 DASSZH010000000
VERSION     DASSZH010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34315"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1819
                     /locus_tag="VFN42_00245"
     CDS             <1..1819
                     /locus_tag="VFN42_00245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HET8995072.1"
                     /translation="AYLREHDGRAILCVANLARTPQAVELELGELQGRIPVELDGGTI
                     FPPIGALPYLLTLPPYGFYWFSLEEEAAWPSQHTPAPELMPDYETLVMRRGLAEALLA
                     ARHSIERDVLPPYLGKRRWYAMKDQTLNAARLALLTPLPDPDLLLAEIETDTPAGTAR
                     WLLPLGIAWEDRQPAPLPAQLALARVRRGARVGLLTDAFALAELAHSILNGLSNGSTI
                     RSDDGEIRFLPTAVMREVTVPPDADMNWLSAEQSNSSVIVGDVAILKLFRRVSDGPHP
                     EAEMGRYLTEQGFSGAAALLGEMVRVGTDGVSHALAIAQSFVRNQGDAFTWTLDLLLR
                     HLSDLAGGDETAAADAERHEDYDAFARLLGCRLGEMHAILARETDNPDFAPVPATHEM
                     AAQWGQQAERQLAAAFAALESQHEWSESATHDMERLAAARESLTQAVHRLAAAADGAV
                     LTRIHGDLHLGQVLVANGAVYIIDFEGEPAKPVEVRRAKNSRLRDVAGMIRSFDYAAA
                     VVERKSRESQAHLADARRTTFLDSFITQATNAFLAGYREAMHITDEAGEQKLLDLFLV
                     EKAAYEVAYEAANRPTWIEVPLRGLARLADELLGGAASE"
     gene            1812..>2137
                     /locus_tag="VFN42_00250"
     CDS             1812..>2137
                     /locus_tag="VFN42_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HET8995073.1"
                     /translation="MNDVADLQGVSADTAWQLANGALHDPFGVLGPHDTAAGRVVRAY
                     LPGARSVEVIARNDKGSLGTLMPMRPDGLFVGRVKSADAYRLRIEWPGAVQEVEDAYS
                     FNRLLL"
BASE COUNT          343 a          744 c          726 g          324 t
ORIGIN      
        1 ggcctatctg cgcgagcacg acggcagggc gatcctgtgc gtcgccaacc tcgcccgcac
       61 gccgcaggcg gtggagttgg aactggggga gctgcagggc cgcatcccgg tcgaactcga
      121 cggcggcacg atctttcccc cgattggcgc gttgccttac ctgctgacat tgccaccgta
      181 cggtttctat tggttctccc tggaagagga agcggcctgg cccagccaac acacgcccgc
      241 tccggaactc atgcccgatt acgaaaccct ggtcatgcgc cgcggcctgg ccgaggcgct
      301 gctcgccgcc cgccacagca tcgaacgcga tgtgctgccg ccgtatcttg gcaagcggcg
      361 ctggtatgcg atgaaggacc agacgctgaa tgccgcgcgc ctggcgctgt tgacgccgct
      421 gcccgacccc gacctgctgc tggcggaaat cgaaaccgac acaccggcgg gcaccgcgcg
      481 ctggctgctg ccgctgggca tcgcctggga agaccgccag ccggcgccgc tgcccgcgca
      541 gctcgcgctt gcccgggtgc gccgcggcgc gcgggtcggc ctgctgaccg acgccttcgc
      601 cctggcggaa ctcgcgcaca gcatcctgaa cggcctgtca aacggcagca cgatccgcag
      661 cgacgacggt gaaatccgct tcctgcccac tgcggtgatg cgtgaggtca cggtgccgcc
      721 ggatgcggat atgaactggc tgtcggcgga gcagtcgaat tcctcggtca tcgtcggcga
      781 tgtcgcgatc ctgaagctgt tccgccgcgt atccgatggt ccgcacccgg aagcggaaat
      841 gggccgctat ctgaccgagc agggattttc cggcgctgcc gcgctgctgg gcgagatggt
      901 acgcgtcggc accgacggcg tctcgcacgc cttggccatc gcgcagagct tcgtccggaa
      961 ccagggcgac gccttcacct ggacgctgga tctgctgctg cgccacctgt ccgacctcgc
     1021 cggcggcgat gaaactgccg ccgccgacgc cgaacgccac gaggactacg acgccttcgc
     1081 ccgcctgctc ggctgccgcc tgggcgagat gcacgcgatc ctggcgcgcg agacggacaa
     1141 ccccgatttc gccccggtgc cggctacgca tgagatggcg gcacagtggg ggcagcaggc
     1201 agagcggcag cttgccgccg ccttcgcggc gctggaaagc cagcacgagt ggagcgaaag
     1261 cgccacgcat gacatggagc gactggccgc ggcgcgcgag tcgctgaccc aggcggtgca
     1321 ccgcctggcc gcggcggccg acggtgcggt gctgacccgc atccatggcg acctgcatct
     1381 cggccaggtg ctggtggcca atggcgccgt ctacatcatc gacttcgagg gcgagcccgc
     1441 caagccggtg gaggtcaggc gggccaagaa cagccggttg cgcgacgtgg cgggaatgat
     1501 ccgctcgttc gactacgccg ccgccgtcgt ggagcgcaaa agccgcgaga gccaggcgca
     1561 tctggccgat gcccgccgca ccaccttcct cgacagcttc atcacccagg caacaaacgc
     1621 cttcctcgcg ggttatcggg aggcgatgca catcaccgac gaagccggcg agcagaagct
     1681 gctcgacctc ttcctggtgg agaaagcggc ctacgaggtt gcttacgaag cggccaaccg
     1741 gccgacctgg atcgaggtgc cgctgcgcgg cctggcgcgt ctagccgacg aactgcttgg
     1801 aggagccgcc agtgaatgat gttgccgatc tccagggcgt ttccgccgac accgcctggc
     1861 agcttgccaa cggcgccctg cacgacccgt tcggcgtgct cggcccgcac gataccgcgg
     1921 ccggacgcgt ggtgcgcgcc tacctgcccg gcgcacgcag cgtcgaggtc atcgcccgta
     1981 acgacaaagg ctcgctcggc acgctgatgc cgatgcggcc ggacgggttg tttgtcgggc
     2041 gggtcaaatc cgcggacgcc tatcggttgc gcatcgaatg gccgggcgcg gtgcaggagg
     2101 tggaggacgc ctacagcttc aaccggctgc tgctcag
//
LOCUS       DASSZH010000014         2973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1449191, whole genome shotgun sequence.
ACCESSION   DASSZH010000014 DASSZH010000000
VERSION     DASSZH010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449191"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="VFN42_00255"
     CDS             complement(<1..1100)
                     /locus_tag="VFN42_00255"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET8995074.1"
                     /translation="MSELHWLTAAEASRAFAARTLSPVALLDALLQRIDRLDPTLHAF
                     IRMDSDAARAAAQAAEREIAAGRIRGPLHGVPVGVKDIIDVAGLPTTCHSKILLDNVA
                     RQDAAVVARLRQAGAIVLGKTATHEFAIGGPSFDLPFPPARNPWNPQHHPGGSSSGTG
                     SGIAGGLFPVGLGSDTGGSVRNPASACGIVGLKPTYGLVSRRGVFPLSFTLDHVGPLA
                     RSVADIALMLDAMAAHDPADPGSAVSNARYFGRMLDRGVRDLRIGFVRHFHETDMPAH
                     PATAAALEEVARVLAAAGAEVRTVALPSLTEFAGVNRVILCSEAWSIHAPWLRTRPGD
                     YARLTRRRLLPGAFMTAGDYVGAQRRRLQMIAA"
     gene            complement(1097..1309)
                     /locus_tag="VFN42_00260"
     CDS             complement(1097..1309)
                     /locus_tag="VFN42_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995075.1"
                     /translation="MIPPGEREGQMDEQLLTMLARAAGLEKALAEFPDDVAAAAAQAL
                     DNAAAFQAPADPAAEPWPPMRAGVGL"
     gene            complement(1640..2902)
                     /locus_tag="VFN42_00265"
     CDS             complement(1640..2902)
                     /locus_tag="VFN42_00265"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008853860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HET8995076.1"
                     /translation="MTIYTGPVFEMARQQFQVIADYLEIPMDERQRLLYPKRAITVAC
                     PIHRDDGRVEVFHGYRVQHHLTLGPTKGGTRFAPNVHIGEIAALAIWMSWKCALTNLP
                     YGGAKGGITVDPRALSRHELENLSRRYMQEMIPFVGPHTDVMAPDMGTNEQIMAWFMD
                     TYSMYQGRTVTEIVTGKPVASGGTLGRREATGRGVAYLALRALERLAIQPVGATAIVQ
                     GFGNVGSYAVRTLAERGLKIVGVSDHTACYYEPRGLDVAALVQHAETHGGVLAGFSNQ
                     IAFDPRELLAQKCDVLVPAAMERVIDAELAARLQCRVLAEGANGPTMPEADTVLDQRR
                     GEVFVIPDILCNAGGVVVSYFEWVQDLQQFFWDEAEVMSKLYHVLDKAFSQVMVRADA
                     DKVPHRTAAMAIGVEKVRSAKNTRGLFP"
BASE COUNT          496 a         1058 c          964 g          455 t
ORIGIN      
        1 gcggcgatca tctgcagccg gcgccgctgc gcgcctacgt agtcgccggc cgtcatgaag
       61 gcgccgggca acagccgccg ccgcgtcagc ctggcgtagt cgcccggccg tgtgcgcagc
      121 cagggcgcgt ggatcgacca cgcctcgctg cacaggatga cccggttcac cccggcgaac
      181 tcggtcagcg acggcagcgc caccgtgcgc acctccgcgc cggccgctgc cagcacgcgc
      241 gccacctcct ccagcgccgc ggcggttgcc ggatgcgccg gcatatcggt ctcatggaaa
      301 tgccgcacga aaccgatgcg caggtcgcgc acgccgcggt ccagcatgcg accgaaatac
      361 cgcgcattac tcaccgcgct gcccgggtcg gccgggtcgt gcgccgccat cgcatccagc
      421 atcagcgcaa tgtcggcgac gctgcgggcc agcggcccga catgatccag cgtgaaggac
      481 aggggaaaca cgccgcggcg cgacaccagg ccgtaggtgg gtttcagccc gacaatgccg
      541 caggcgctgg cggggttgcg taccgagccg ccggtgtcgg agccgagccc cacggggaac
      601 agcccgccgg cgatcccgga cccggtgccg gatgacgacc cgccgggatg atgctgcgga
      661 ttccacggat tgcgcgccgg cgggaacggc aggtcgaagc tcgggccgcc gatggcgaat
      721 tcgtgggtgg cggtcttgcc cagcacgatc gcgccggcct ggcgcagcct ggcaaccacc
      781 gcggcatcct gccgggccac gttgtccagc aggatcttcg agtggcaggt ggtgggcagc
      841 ccggccacgt cgatgatgtc cttcaccccc accggtaccc cgtgcaacgg cccgcggatg
      901 cgaccagcgg cgatctcccg ttccgccgcc tgcgccgccg cgcgtgccgc gtccgaatcc
      961 atccggatga aggcatgcag cgtcgggtcc aaccggtcga tccgctgcag cagcgcgtcg
     1021 agcaacgcta ccggcgacag cgtgcgggcc gcaaaggcgc gcgacgcctc ggcggcggtc
     1081 agccagtgca gttcgctcac aacccgaccc ccgcgcgcat cggcggccac ggttcggcgg
     1141 ccggatcggc cggtgcctgg aacgccgcgg cattgtccag cgcctgcgcc gccgcggctg
     1201 ccacgtcgtc gggaaattcc gccagcgcct tctccagtcc ggccgcacgc gccagcatcg
     1261 tcaacaactg ctcgtccatc tgcccctcgc gttccccagg agggatcatg cgacggtgcc
     1321 gcacccggcg gaagggccgc ggcgcgaccc gtgctgcgtc actgtgctgt gcaacgtgca
     1381 acatccggca ggagatctgg ggattatcac gatgccgcat accggagcga cggcgctgcc
     1441 gggcgcttcg gccgaacatg tgcagcgcaa gaaaaatcgg cccgaccaag gcggtcgggc
     1501 cgtaggaagg tccgggttga cctgggccat ggagacccag ccagccggcg caggtgttgg
     1561 ggcagcggcg gctgacagct gcgttatcgc ccgctgccgc cgaacccgct gtgaagcagg
     1621 tcacacaacc ggcggattcc tatgggaaca gccctctggt gttcttggca ctgcgcacct
     1681 tctccacgcc gatcgccatc gccgcggtcc ggtgtggcac cttatcggca tccgcccgca
     1741 ccatgacctg gctgaacgcc ttgtccagca cgtggtagag tttgctcatc acctcggctt
     1801 catcccagaa gaactgctgc aggtcctgca cccattcgaa gtagctgacc accacaccgc
     1861 cggcattgca caggatgtca gggatgacga acacctcgcc gcgccgctgg tccagtacgg
     1921 tgtcggcttc cggcatggtg gggccattgg cgccctcggc cagaacgcgg cattgcagcc
     1981 ttgccgccag ttcggcatcg atcacccgct ccatcgccgc cggtaccagc acatcgcact
     2041 tctgtgccag caattcgcgc ggatcgaagg caatctgatt ggaaaagccg gccagcaccc
     2101 cgccatgcgt ctcggcgtgc tgcaccaggg ctgccacgtc gaggccacgc ggctcgtaat
     2161 agcaggcggt atggtcggaa acgccgacga tcttcaggcc gcgctccgcc agtgtgcgca
     2221 ccgcgtagct gccgacattg ccgaaaccct gcacgatcgc ggtggccccg accggctgga
     2281 ttgccagccg ctccagtgcc cgcaacgcga gataggcgac gccgcggccg gtggcttcgc
     2341 gccgccccag cgtgccgccg gaggccaccg gtttgccggt gacaatctcg gtcaccgtgc
     2401 ggccctggta catggagtag gtgtccatga accaggccat gatctgctcg ttggtgccca
     2461 tatcgggcgc catcacatcg gtatgcggac cgacgaaggg gatcatctcc tgcatgtagc
     2521 ggcgcgacag attctccagc tcatgccgcg acagcgcgcg cggatcgacg gtgatgccgc
     2581 ccttggcgcc gccatagggc agattggtca acgcgcattt ccagctcatc cagatggcca
     2641 gcgcggcgat ctcaccgata tgcacgttcg gggcaaaccg cgtgccgcct ttggtgggcc
     2701 ccagcgtcag atgatgctgc acacgatagc cgtgaaacac ttccacccgg ccatcatcgc
     2761 ggtggatggg gcaggcgacc gtgatggcgc gcttcggata gagcagccgc tggcgttcat
     2821 ccatcgggat ttccagataa tcggcaatca cctggaactg ctgacgggcc atttcgaaca
     2881 ccggaccggt gtagattgtc atccgcttcc tcctggcacc cacgccaatc tggcgcacca
     2941 tatctcaatc gcaagagcca aacgtctgcc ggc
//
LOCUS       DASSZH010000015         2278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_869638, whole genome shotgun sequence.
ACCESSION   DASSZH010000015 DASSZH010000000
VERSION     DASSZH010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869638"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..1487
                     /gene="ccoG"
                     /locus_tag="VFN42_00270"
     CDS             33..1487
                     /gene="ccoG"
                     /locus_tag="VFN42_00270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HET8995077.1"
                     /translation="MSRVSKSDSEPQPPASLYASRVRVYPKSVHGPVRRFKWAVLIAC
                     LAVYYLLPWLRWHRGVTEPDQAVLLSLWHERFYLFNLEFWPQDIYYLAVLLIMGAVAL
                     FLVTSLVGRVWCGYTCPQTVWTDLFMWVERLIEGDRNERMKRDAQPTSFDKLWRKTAK
                     HAVWLGIAFWTGGAWIMYFVDAPTVTVEFWTGTASKEVYFFTFLFTATTYALAGWARE
                     QVCTYMCPWPRFQAAMLDEQSLTVTYQSWRGEPRTRGKRPADSKAGDCVDCAACVTAC
                     PTGIDIRDGIQLECINCGLCIDACNHVMAKQGKPRWLITWDTLANQAAKERGEHAPFR
                     FLRARTIIYLSALLLGAAVFTVTLATHASQHLSVLHDRAPLFVRLADGRVRNGYTIKI
                     VNKQTVPVTFELSVEGMPDAILTEPDENLGPAAHLGLPVDADSVGTYRVLVAANPAHL
                     HDGARHLDFILRNPVTGEQSEYDSVFMGPAETHQ"
     gene            1500..2009
                     /locus_tag="VFN42_00275"
     CDS             1500..2009
                     /locus_tag="VFN42_00275"
                     /inference="COORDINATES: protein motif:HMM:NF017559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HET8995078.1"
                     /translation="MRYKDGLQALHGGTPGARSSWRWFPWAITAAMLVVIGVNIGMVY
                     AAMHSFPGAAGGDGFDLSNRYDRVIDQVQRQAALGWSLQASADALGHPVLLLHDRAGA
                     PLAGAVLRGTAERPVGPQDATALAFQELAPGRYVAQLPLSERGQWDLLVSATAEGHRY
                     TTTRRIMVR"
BASE COUNT          356 a          753 c          775 g          394 t
ORIGIN      
        1 cggccatgac gttcgcctgc tggatgcgct gaatgtcccg cgtcagcaaa tccgacagcg
       61 agccgcagcc gccggccagc ctgtacgcca gccgggtgcg cgtctacccg aagtcggtgc
      121 atggcccggt gcgccgcttc aaatgggcgg tcctgatcgc ctgcctcgcg gtctactacc
      181 tgctgccctg gctgcgctgg catcgcggcg tgaccgagcc cgaccaggcg gtgctgctca
      241 gcctgtggca cgagcgcttc tatttgttca atctcgagtt ctggccacag gacatctact
      301 acctcgccgt gctgctgatc atgggcgcgg tggcgctgtt cctggtcacc agcctggtcg
      361 gccgcgtgtg gtgcggctac acctgcccgc agacggtgtg gaccgacctg ttcatgtggg
      421 tcgagcgcct gatcgaaggc gaccgcaacg agcgcatgaa gcgcgacgcg cagccgacgt
      481 cgttcgacaa gctgtggcgc aagacggcga aacacgccgt gtggctgggc atcgccttct
      541 ggaccggcgg tgcctggatc atgtacttcg tcgatgcacc gacggtgacg gtagaattct
      601 ggaccggcac cgcgtcgaag gaagtgtatt tcttcacctt cctgttcacc gccaccacct
      661 acgcgctggc cggctgggcg cgcgagcagg tgtgcaccta catgtgcccc tggccgcgct
      721 tccaggcggc gatgctggat gagcagagcc tcaccgtcac ctaccagtcc tggcgcggcg
      781 aaccgcgcac ccgcggcaag cgcccggcgg acagcaaagc cggcgactgc gtcgattgcg
      841 ccgcctgcgt caccgcctgt ccgaccggga tcgacatccg cgacggcatc cagttggagt
      901 gcatcaactg cggcctgtgc atcgatgcct gcaatcatgt catggcgaag cagggcaagc
      961 cgcgctggct gatcacctgg gacacgctgg ccaatcaggc ggcgaaggag cgcggcgagc
     1021 atgcaccgtt ccgcttcctc cgtgcccgca ccatcattta tctctccgcc ctgctgctgg
     1081 gtgcggcggt gttcacggtg acgctggcga cgcatgccag ccagcatctg tcggtgctgc
     1141 acgaccgtgc gccgctgttc gtgcgcctgg ccgacggcag ggttcgcaac ggctacacca
     1201 tcaagatcgt caacaagcag accgtgccgg tgaccttcga attgtcggtg gagggcatgc
     1261 ctgacgcgat cctgaccgaa cccgatgaaa atctcggccc ggcggcgcat ctcggcttgc
     1321 cggtggacgc agacagcgtg gggacctacc gcgtgctggt ggcagcaaat cccgcgcatc
     1381 tgcacgacgg cgcgcggcat ctggacttca tcctgcgcaa tccggtgacc ggggagcagt
     1441 ccgaatacga ttcggtattc atgggcccgg cagagactca tcaatagggg cgaggaccaa
     1501 tgcggtacaa agacggattg caggcactgc atggcggcac gcccggagcg cgttcgtcct
     1561 ggcgctggtt tccctgggcg atcaccgcgg cgatgctggt ggtgatcggg gtgaatatcg
     1621 gcatggtgta tgcggccatg cacagttttc ccggcgccgc cggcggcgac gggttcgacc
     1681 tgagcaaccg ctatgacagg gtgatcgacc aagtgcagcg ccaggcggcg ctgggctgga
     1741 gcctgcaggc ctccgccgac gcgcttgggc atccggtgct gctgctgcac gatcgcgccg
     1801 gcgcgccact ggcaggcgcg gtgctgcgcg gcacggcgga acgcccggtg ggtccgcagg
     1861 atgcgaccgc gctagccttc caggaactgg cgccggggcg ctacgtcgca cagcttccgc
     1921 tgagcgagcg cgggcagtgg gatctgctgg tctcggcgac cgcggagggc catcgctata
     1981 cgaccacgcg gcggatcatg gtgcggtgac gcttttgtct gctgcggccg gtgttcctga
     2041 acgtgtcatg gctgggcttg acccggccat ccaccccggg aaagagcgga ccgcattact
     2101 acggaacacg tccggtcggg ggcaggtggc cgggtcaagc ccggccacga cgggtttgga
     2161 cagtgccgat ccgccggcaa actgcgccca ttgcggcctg cccgccgccc ccggccaccg
     2221 gttctgctgc cccggctgcg ccgcggctta tgacatcatc caggggcttg ggctgggg
//
LOCUS       DASSZH010000016         4580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1517333, whole genome shotgun sequence.
ACCESSION   DASSZH010000016 DASSZH010000000
VERSION     DASSZH010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517333"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..1501
                     /locus_tag="VFN42_00280"
     CDS             182..1501
                     /locus_tag="VFN42_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HET8995079.1"
                     /translation="MRRVVLHSETDWDGWRMATRKLVLAGVAPDQVRWSVRAGPDDAS
                     DGVPEGDGGFTLSRSLVAMAALAFQAREPERFALLYGLVWRANAGAKLDADDPDLRRA
                     QGLAFAVRAEAHRMRTNLRFLPVEGGDGLDHVGWYAPAHYVLEANAQLLATRFSRHRC
                     AVLTPDGSALWGDGELRFAGGVATIEDDAALSAWWAAHRATLRHGPHRGTSIPPAEDL
                     DEAPRPPDRLPLGPVVLPAGADTGLLEASHEAGDCRRCDLHGPATQTVFGEGPAHARV
                     MFIGEQPGDQEDVIGRPFVGPAGQMLDRALEEAGIDRRTVYVTNAVKHFKFTPRGKRR
                     IHQPPDVGEIAACRFWLDVERVRLQPKLLVLMGGSAARAVLNRAVTISRERGRPITME
                     DGQTAFVTVHPSYLLRIPDADAKAREYRAFVRDLTAIRALIAGLQTA"
     gene            complement(1498..1977)
                     /locus_tag="VFN42_00285"
     CDS             complement(1498..1977)
                     /locus_tag="VFN42_00285"
                     /inference="COORDINATES: protein motif:HMM:NF013981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HET8995080.1"
                     /translation="MQSLRILVLLGVANLAPILATRLLGTRYATPLDGGRLMPDGRPV
                     FGAGKTLRGVLISILCTTLAAPLLALDALLGTGLAAGAMAGDLLASFCKRRLGLPAHA
                     RAFGLDQVPEVLLPLLLLQPWLSLEWREIAVVVVVFVLLDILLSRILYHLSIRERPY"
     gene            complement(1977..2717)
                     /locus_tag="VFN42_00290"
     CDS             complement(1977..2717)
                     /locus_tag="VFN42_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HET8995081.1"
                     /translation="MAPPRLTDFRVLSFDCYGTLIDWETGIATALQPLLAQVPAIPRD
                     AALEAFARHETRLEAEAPATLYRDLLARVHAALALEWRVPPDAGADQRFAASVADWPA
                     FPDSVTALRYLKQHYKLAILSNVDRDSFRGSQARLGVAFDLVCTAQDVGSYKPDPKNF
                     RYLIARLGEQGIAQHEILHVAQSLFHDHAPANALGLASAWIDRRHASGGWGATSAPPA
                     GVHYDFRFTSLEDLAAAHRQAHSAADSG"
     gene            complement(2766..3869)
                     /locus_tag="VFN42_00295"
     CDS             complement(2766..3869)
                     /locus_tag="VFN42_00295"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8995082.1"
                     /translation="MNAPVDVLQRQTAADAKLGIIDCDIHPYPKPGAFNPYLPERWRK
                     HIAEYGKFNCGVYADRITYPRFQPNLSRRDAWPPNGGPPGSDIGFIREQLLDAYNIAY
                     GVLEPLLGGNTSRNLEEAAALCSAMNDWQAYDFVDAEPRLRASILVPQDDPEAAVKEI
                     EKRAGDWRFAQIQLGSKTSEPVGRKRYWPIFAAAQANGLSIGLHVGGTQSSAPSAGGW
                     PQFYIEEHHGLVHSMQNQAASLILEGVFEAFPKLKVVLIEGGFGWVPSWTWRLDKLWA
                     KMRDEVPQVKRPPSEYLRSNVWFATQPVEEPENPEDLRQVFEWIGWDRMVYSSDYPHW
                     DFDDPFQAFHIRMTPEEQRMVLRENALNVYAFR"
     gene            complement(3866..>4580)
                     /locus_tag="VFN42_00300"
     CDS             complement(3866..>4580)
                     /locus_tag="VFN42_00300"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8995083.1"
                     /translation="LDKEPRLRASIVVPAQAPLLAAEEIERRAADRRFVQVLLPVACE
                     MMLGRSYYWPIYEAAVKHDLPIGLHAGSMYRYAPTATGWPSHYLHDYAAQSQIFEDQL
                     LSLVSNGIFNKFPTLRVVLIESGVTWLPGFLWRAIKTWRGVRAEVPWVSRSPAEIIRD
                     NVRLTTQPIDAPPDPRVINRIVEQIGDDRMLLFASDYPHWQFDGDDPLPAGLSPDLLR
                     RIQRDNPLETYPRLKETVQ"
BASE COUNT          762 a         1440 c         1607 g          771 t
ORIGIN      
        1 caactgagga aagtcgtgga tgccggcctt cgccggcatg acggaattct gcgtgccgaa
       61 gagtcgagct catcgcgggt tggtattatt ccacgggctt gccggagcgc aagcctggga
      121 ctgggtccga catcccaggc ccggacttgg aaaccggcgc gcattgcggt ttgctgcggc
      181 catgcgacgg gtggtgctgc acagcgagac cgactgggac ggatggcgca tggccacccg
      241 gaagctggtc ctcgccgggg tggcgccgga ccaggtccgc tggtcggtgc gagccggacc
      301 cgacgatgcc tcggacggag taccggaggg tgatggcgga ttcactctgt cgcgttccct
      361 ggtggcgatg gcggcgctgg cctttcaggc gcgggagccg gaacgcttcg cgctgctgta
      421 cggtctggtg tggcgcgcca atgccggtgc gaagctggac gcggacgatc cggatcttcg
      481 ccgggcgcag ggcctggcgt ttgccgtgcg ggccgaggcg cacaggatgc gcaccaatct
      541 gcgcttcctg ccggtcgagg gcggcgacgg tctggaccat gtcggttggt acgcgccggc
      601 gcattatgtg ctggaggcaa atgcgcagct gctcgcaacc cgtttctccc ggcatcgctg
      661 tgccgtgctg acgcccgatg gcagtgcatt gtggggcgac ggagagttgc gtttcgccgg
      721 tggcgtggcg acgatcgagg atgacgcggc cttgtcggca tggtgggcgg cgcatcgtgc
      781 gacgctgcga cacggcccgc accgcggcac gtccattccg cccgccgagg atctggacga
      841 agctccgcgt ccgccggacc gcctgccgct tggtcctgtc gtgctgccgg cgggagcgga
      901 taccggattg ctggaagcgt cgcacgaagc cggcgattgc cgccgctgcg acctgcatgg
      961 acctgcaacc cagaccgtgt tcggcgaagg tccggcgcat gcccgggtga tgttcatcgg
     1021 cgagcagccg ggcgaccagg aagacgtgat cggccgcccc ttcgtcggcc ctgccgggca
     1081 gatgctggac cgtgctttgg aagaagccgg catcgaccgc cgcacggtgt atgtcaccaa
     1141 tgcggtgaag cacttcaagt tcactccgcg tggcaagcgc cgcatccatc agccgccgga
     1201 tgtcggtgag atcgccgcct gccgtttctg gctggatgtg gagcgggtaa ggcttcagcc
     1261 gaagctgctg gtgctgatgg gcggcagcgc ggcgcgtgcg gtgctgaatc gcgcggtcac
     1321 catcagccgc gagcgcggcc ggccgatcac gatggaggat gggcagacgg cgttcgtgac
     1381 cgtacatcca tcctatttgc tgcgtattcc ggacgcggac gccaaggcgc gcgaataccg
     1441 ggccttcgtg cgcgacctca cggcgatacg ggcgttgatt gcgggattgc agacggccta
     1501 gtacggccgc tcgcggatgc tcaggtggta gaggatccgc gacagcagga tgtccagcag
     1561 cacgaagacc accaccacga cggcaatctc gcgccactcc agcgacagcc agggctgcag
     1621 cagcagcagc ggcagcagca cctcgggtac ctggtccagg ccgaaggcgc gcgcatgggc
     1681 gggtaacccg agccggcgct tgcagaaact ggccagcagg tcgcctgcca tggcgccggc
     1741 cgcgagcccg gtgccaagca gcgcatccag cgccaacagc ggcgcggcaa gcgtggtgca
     1801 caggatcgag atcagcacgc cgcgcaatgt cttgccggcg ccaaacaccg ggcggccatc
     1861 cggcatcagg cgaccgccat cgagcggtgt ggcgtagcgt gttcccagca gccgggtggc
     1921 aaggatgggc gccaggtttg ccacgccgag cagcaccagg atccgcaggc tttgcatcag
     1981 ccggaatcgg cggcgctgtg cgcctggcga tgcgcggccg cgagatcttc caggctggtg
     2041 aagcggaaat cgtaatgcac cccggcgggc ggcgcactgg ttgcgcccca gccgccggaa
     2101 gcatgccgcc ggtcgatcca ggcggaggcc aagccgagcg cgttcgccgg cgcgtggtcg
     2161 tgaaacaggc tttgcgcgac gtgaaggatt tcatgttgcg cgatcccctg ctcgcccagg
     2221 cgtgcgatca ggtagcggaa gttctttgga tccggcttgt aggacccgac atcctgcgcg
     2281 gtgcatacca ggtcgaaggc aacgcccagc cgcgcctggc tgccgcgaaa gctgtcgcgg
     2341 tccacgttgg acaggatggc aagcttgtag tgctgcttca ggtagcggag cgccgtgacg
     2401 ctatccggaa atgccggcca gtcggcaacc gaggcggcaa agcgctggtc cgcgccggca
     2461 tccggcggca cgcgccattc cagcgcaagc gcggcatgca ccctggcgag caggtcgcgg
     2521 tacagcgtgg cgggggcctc ggcctccagc cgcgtttcgt ggcgggcaaa ggcttccagt
     2581 gcggcatcgc gtgggattgc cggtacctgg gccagcagcg gctgcaacgc ggtggcgata
     2641 cccgtctccc agtcgatcag cgttccatag cagtcgaagc tgagaacgcg gaaatcggtc
     2701 agacgaggtg gcgccacgcg attgtctccg gcaactcgtt ggggggaaac ccgccgcaac
     2761 gggttctatc gaaatgcgta cacgttgagc gcgttctcgc gcaggaccat ccgctgctcc
     2821 tctggcgtca tgcggatgtg gaaggcctgg aacggatcat cgaaatccca gtggggataa
     2881 tcgctggaat acaccatgcg gtcccagccg atccattcga agacctgtcg caggtcttcc
     2941 gggttttccg gttcctccac cggctgcgtg gcaaaccaga cattgctgcg caggtactcg
     3001 gaaggcggac gcttcacctg cggcacttcg tcgcgcatct tcgcccacag cttgtccagc
     3061 cgccatgtcc aactgggtac ccagccgaag ccgccctcga tcagcaccac cttcagcttg
     3121 ggaaacgcct cgaacacgcc ttccaggatc aggctcgccg cctggttctg catggagtgc
     3181 accagaccgt ggtgttcctc gatgtagaat tgcggccagc cgccggcgga tggcgcgctg
     3241 gactgcgtgc cgccgacatg cagcccgatc gacaggccgt tcgcctgcgc ggcggcgaag
     3301 atcggccagt agcgcttgcg gccgaccggc tcggaggttt tcgagccgag ctggatctga
     3361 gcaaaacgcc agtcgccagc acgcttttcg atttccttca ccgcggcctc cggatcgtcc
     3421 tgcggcacca ggatggacgc gcgcagcctg ggttcggcat cgacaaagtc gtacgcctgc
     3481 caatcgttca tcgcgctgca cagcgcagcg gcttcctcca gattacggga ggtgttgccg
     3541 cccagcagcg gttccagcac gccgtacgcg atgttgtagg cgtccagaag ctgttcccgg
     3601 atgaagccga tatccgagcc cggcgggccg ccgttcggcg gccaggcatc gcggcgggac
     3661 aaattcggct ggaagcgggg ataggtgatg cggtctgcat agacgccgca gttgaacttg
     3721 ccgtattcgg cgatgtgctt gcgccagcgc tccggcaaat acgggttgaa tgcgcccggt
     3781 ttcggatagg ggtggatgtc gcagtcgata atgccgagct tcgcatcggc tgcggtttgc
     3841 cgctgcagga catcgacggg tgcgttcatt gcacggtctc cttcaggcgg ggataggtct
     3901 ccagcggatt gtcgcgctgg atgcggcgca acagatcggg tgacaggccg gcgggcagcg
     3961 ggtcgtcgcc gtcgaactgc cagtgcggat agtcggaggc gaacagcagc atgcgatcgt
     4021 cgccgatctg ctcgacaatg cgattgatca cccgcggatc gggcggcgcg tcgattggct
     4081 gcgtcgtcag gcgaacgttg tcgcggatga tctccgccgg agaacggctg acccagggca
     4141 cttcggcgcg cacgccgcgc caggtcttga tggcgcgcca caggaagccg ggcagccagg
     4201 tgacgccgga ttcgatcagc accacgcgca gggtcgggaa cttgttgaag atgccgttgc
     4261 tgaccaggct gagcagttgg tcctcgaaaa tctgcgattg cgccgcgtag tcgtgcagat
     4321 agtgcgaggg ccagccggtg gcggtcggcg cgtaacggta catgctgccg gcatgcagcc
     4381 cgatgggcag gtcgtgcttc accgccgctt catagatcgg ccagtaatag ctgcggccaa
     4441 gcatcatctc gcaagccacc ggcagcagga cctgcacaaa gcggcgatcg gccgcgcggc
     4501 gttcgatttc ctctgccgcg agcagtggcg cctgcgcggg caccacgatg gaggcgcgca
     4561 gccgcggctc cttgtccagc
//
LOCUS       DASSZH010000017         3199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_68561, whole genome shotgun sequence.
ACCESSION   DASSZH010000017 DASSZH010000000
VERSION     DASSZH010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68561"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2133)
                     /locus_tag="VFN42_00305"
     CDS             complement(<1..2133)
                     /locus_tag="VFN42_00305"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET8995084.1"
                     /translation="MHVQSGNTGIGQPIRRREDQRLITGRGRYSDDAKLPGAAYAVML
                     RAPHARATIAGIDTAAARAMPGVLAVLTGADMRQDGLQPIPHAVWSRHPAELDLPNAD
                     GSEAPIPPHYCMATDEVRHVGEIVAMVVAESVAAAKDAAEQVQVAYRAGPVVTQALAA
                     AQPDAPAARNDLAGNVLIDSLLGDARATEAAFARAAHTAQLTTWIQRVAGVPMEPRAA
                     LGEYDAATGHYTLHAGAGGAARPKGDLVTVLGVPADKLRMVMHEVGGNFGTRGATNPE
                     FALVLWAARRVGRPVKWTCERSEYFTCDYQARDLAVTAELALDAKGNFLAMRGSLLSN
                     VGAYPIAFGPLQKGMEIVSSIYHVPSVLFRARAAVTNTVPTRPYRSSGRPEVMYVMER
                     LIDLAARNSGLDRIALRRRNLIPQSALPYRNPMGMIYDSGDYGGVMERTLELADWSGF
                     AARRAAAAARGRCRGIGIANYVDTATGVPRERAEVTIQPEGVVEVVIGTVSQGQGHET
                     SFAQLMVEWLGVPLDSVQLVTGDTARVSVGGGAHSGRALRLGSIVMLNASRLILDKAQ
                     RIASHVLEVAPGDLEFAAGRFTVAGTDRSLGLFEIARVARERNDLPEELRGKLDAISD
                     ETIPEASFPYGCHVCEVEVDPETGVTRIERYTAVDDVGRAVNPMIVHGQVHGGIVQGV
                     GQAMGEQCVYDPDSGQLLSGSFMDYTMPR"
     gene            2730..>3199
                     /locus_tag="VFN42_00310"
     CDS             2730..>3199
                     /locus_tag="VFN42_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995085.1"
                     /translation="MTIEPSTAAPTKPPGQTGGRAVPEGITRVLVLLRWIIGYGRNLA
                     DTFHQRAADAAFRDFAAIRFHTPDLNAIFARVRRGLMLALALEAKLLKRAATGRELVE
                     APVRSGTPPARPSGTARKPHAARRSKLIDLPLDHLPTAQQIADELRRRPLGAILV"
BASE COUNT          522 a         1147 c          986 g          544 t
ORIGIN      
        1 gcggggcatt gtgtagtcca tgaaggagcc ggacagcagt tgtccgctgt caggatcata
       61 cacgcattgc tcgcccatcg cctggccgac gccctgcacg atgccgccat gcacctggcc
      121 gtgcacgatc atcgggttca ccgcgcggcc gacatcgtcc accgcggtat agcgctcgat
      181 ccgcgtcacg ccggtctccg gatcgacttc cacttcgcag acgtggcaac cgtagggaaa
      241 gctcgcttcc gggatggttt cgtcgctgat ggcgtcgagc ttgccgcgca gctcctccgg
      301 cagatcgttg cgttcccgcg ccacccgcgc gatctcgaac aggcccagcg agcggtcggt
      361 gccggcgacg gtgaagcggc ctgcggcaaa ctccaggtcc cccggggcaa cttccagcac
      421 atggctggcg atgcgctgcg ccttgtccag gatcagccgc gacgcattca gcatgacgat
      481 gctgcccagc cgtagcgcgc gcccggaatg cgcgccgccg ccaaccgaga cccgcgcggt
      541 atcgccggtc accagttgca cgctgtccag cggcacgccg agccattcca ccatgagctg
      601 cgcgaagctg gtctcgtgac cctggccttg cgacacggtg ccgatcacca cctccaccac
      661 gccctcgggc tggatggtca cctcggcgcg ctcgcgcggc acgccggtgg cggtatcgac
      721 gtagttggca atgccgattc cgcggcagcg tccccgcgcg gcggctgccg cccggcgcgc
      781 tgcgaagccg ctccaatccg ccagttccag cgtgcgttcc atcacgccgc catagtcgcc
      841 gctgtcgtag atcatgccca tcggattgcg gtacggcagc gcggattgcg ggatcaggtt
      901 gcgccggcgc agcgcgatgc gatcgaggcc gctgttgcgc gcggccagat cgatcagccg
      961 ttccatcaca tacatcacct ccggccgccc gctgctgcgg tacgggcggg tcggcacggt
     1021 gttggtgacc gcggcgcgcg cacggaacag caccgacggc acgtgataga tgctggagac
     1081 gatctccatg cccttctgca gcgggccgaa ggcgatcggg taggcgccga cattgctcag
     1141 cagcgaaccg cgcatcgcca ggaaattgcc cttggcatcc agcgcgagtt ccgcggtcac
     1201 cgcgaggtcg cgcgcctggt agtcgcaggt gaagtattcc gagcgctcgc acgtccactt
     1261 caccgggcgg ccgacgcggc gcgcggccca cagcaccagc gcgaattccg gattggtggc
     1321 gccgcgcgtg ccgaaattgc cgccgacctc atgcatcacc atccggagct tgtcggccgg
     1381 gacacccagc acggtgacca ggtcgccttt cggccgtgcc gccccgccgg cgccggcatg
     1441 cagcgtgtag tggcccgtcg cggcatcgta ttcgcccagc gccgcgcgcg gttccatcgg
     1501 cacgccggcg acgcgctgga tccaggtggt gagctgtgcg gtatgcgccg cgcgggcgaa
     1561 cgcagcctcc gtggcgcgcg catcgcccag cagcgagtcg atcaacacgt tgccggccag
     1621 gtcattgcgg gccgccggcg catccggctg tgccgccgcc agcgcctgcg tcaccaccgg
     1681 cccggcacga tacgccacct gcacctgctc cgccgcatcc ttcgccgcgg cgacgctttc
     1741 ggccaccacc atggcgacga tttccccgac atgccgcacc tcatcggtgg ccatgcagta
     1801 atgcggcggg atcggcgctt ccgatccgtc cgcgttcggc aggtcgagtt ccgcaggatg
     1861 ccgcgaccac accgcatgcg ggatcggttg cagcccgtcc tgccgcatat ccgcgccggt
     1921 gagcaccgca agcacgccgg gcatggcacg cgccgccgcg gtgtcgatgc cggcaatggt
     1981 ggcgcgggcg tgcggcgcgc gcagcatcac cgcataggcc gcgccgggca gcttggcatc
     2041 gtcgctgtag cggccgcggc cggtgatcag gcgctggtcc tcgcgccggc ggatcggctg
     2101 gccaattccg gtgttcccgg actgcacatg catgcggttt cctcccacgg atgcgccatt
     2161 cggcgcgtcc catggcgagt ataggcggat cgatccagcg caggaaaatg cggctccgcc
     2221 tgacggagca tggagtgctg gggacaggct gctggcgggg ccgttgcgaa tatgcgagtg
     2281 agggcacaag aaatcgaata aataagaaat aaataaagca atacacagag tttcacgaag
     2341 ttcacacaaa gtttcacgaa gtataatatt gttatttatc aacaaaaaaa tactgttacg
     2401 gctgggcagg tggcgccgct gccgctgaat acgtttcggt atcagcaatg ccgccgttcc
     2461 acattttgcg aagatatttc ttagtgaaac tctgtgtgca ctttgtggaa ctctgtgtat
     2521 tacttgattg tttcctttct ttcttactct tagtttttga agcgagcgcc accaactccg
     2581 ccgtgatgcc cccgcccgcc gccgcccccg aacgcagaaa ccgtccccac cgacgcagcg
     2641 acctaggcga ccccccacgc cggaacgtgc caggattttt tgcaagaccc cccttgactt
     2701 ttcttcctag atgtgttctt attacgttca tgaccatcga gccctccacc gccgcgccga
     2761 ccaagcctcc cgggcaaacg ggcgggaggg ccgtgcccga gggcatcacc cgcgttctcg
     2821 tcctgctgcg ctggataatc ggctacggca gaaatctcgc cgacaccttc caccagcgtg
     2881 ccgcggacgc ggcgttccgc gacttcgccg ccatccgctt ccacaccccc gaccttaatg
     2941 cgatctttgc ccgcgtccgg cgcggcctga tgctcgcctt ggcgctggag gccaaactcc
     3001 tcaaacgggc tgccaccggc cgcgagcttg tcgaggcgcc ggtccgctcc ggcacgcccc
     3061 ccgcccggcc ctccggcacg gcccgcaagc cccacgcggc ccggcgcagc aaactcatcg
     3121 acctgccgct ggaccacctg cccaccgccc aacaaatcgc cgacgaactc cgccgccgcc
     3181 cgctcggcgc catcctggt
//
LOCUS       DASSZH010000018         2633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1619688, whole genome shotgun sequence.
ACCESSION   DASSZH010000018 DASSZH010000000
VERSION     DASSZH010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619688"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1290)
                     /locus_tag="VFN42_00315"
     CDS             complement(100..1290)
                     /locus_tag="VFN42_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HET8995086.1"
                     /translation="MSSSLPNDPTTAIRSIAERIEPQLIEIRRDIHAHPELGFEEVRT
                     AGVVARELTRLGIPHQTGIGKTGVVGLIEGGRPGPVLAIRADMDALPIQEETGLPFAS
                     QNPGLMHACGHDIHTTTLIGVAEVLKEIAPQLAGTVKLVFQPAEEGVGGMRAMIADGL
                     MLSPKIDMALGFHNHPDMAVGHFGFVHGACLAASDRFDIVVRGRSGHAAYPHGAIDPV
                     IAACFLVAQLQTVVSREVKPLHPAVVTVGAINGGTTHNIIPDTCTLKGTVRTLHPEAR
                     DAAEAAIKRLAAGMLEGMRVQCEVNYRRGVPPLRNDDRVLEPAVAAVRKQFGDVVEEG
                     EPSLGGEDFALMADLVPSFQLKVGSAQPGRRDRLHNSGYQPDERCIANGVQALSRAAL
                     ELLG"
     gene            complement(1361..1672)
                     /locus_tag="VFN42_00320"
     CDS             complement(1361..1672)
                     /locus_tag="VFN42_00320"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995087.1"
                     /translation="MKYRFAPVVLVLAALLAGCATETTSTGNPYPPVPAPLAEHRPLP
                     PVSAQALMWQPGHWDWNGSGYVWQKGQYVPAAGHGPLFQPGYWAQTPSGWSWQPAHWT
                     S"
     gene            complement(1752..>2633)
                     /gene="ispF"
                     /locus_tag="VFN42_00325"
     CDS             complement(1752..>2633)
                     /gene="ispF"
                     /locus_tag="VFN42_00325"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HET8995088.1"
                     /translation="EALVPHAPDIVLVHDAARPCIPPGTVPALLAALEQAAGAIPGLP
                     VADTLKRVGRGVIMETVPRDGLYRAQTPQAFRFPVLLAAHRAGIAGATDDASLLEAAG
                     ETVAIVPGSEDNIKLTYPEDLARLERIMAPTLIPRVGTGFDVHVLEPGRKLMLCGVEV
                     PHDQGLAGHSDADVGIHALCDAIYGALAEGDIGRHFPPSQAAWKDADSARFLRHAAER
                     IAARGGMLANADVTLICERPKIAPHAAAMMARLAALLGVDPGRVSVKATTTERLGFTG
                     RGEGIAAQAIATILLPS"
BASE COUNT          408 a          934 c          900 g          391 t
ORIGIN      
        1 gccccctccc cccatccttg gtccgcttcg cggcccaagc ccgcaagggg agggggagga
       61 gtctggatgg ccgttcttcc ccacccccgg cggcatccgc tagcccagca actccagcgc
      121 ggcgcgggac agggcctgca cgccgttggc gatgcagcgc tcgtccggct ggtagccgga
      181 attgtgcagc cggtcgcggc ggccgggctg cgccgaacca accttcaact ggaaggacgg
      241 caccaggtcc gccatcagcg cgaaatcctc gccaccgagg ctgggctcgc cttcctccac
      301 cacatcgccg aactgcttgc gcaccgcggc gaccgcgggc tccagcacgc ggtcatcatt
      361 gcgcagcggc ggcacgccgc ggcggtaatt cacctcgcac tgcacgcgca tgccttccag
      421 catacccgct gccagccgct tgatcgccgc ctccgcggca tcgcgcgctt ccgggtgcag
      481 cgtgcgcacc gtacctttca gcgtgcaggt gtccgggatg atgttgtgcg tggtgccacc
      541 gttgatcgca ccgaccgtca ccaccgccgg atgcagcggc tttacctcgc gcgacaccac
      601 ggtttgcagc tgcgccacca ggaaacatgc ggcgatgacc gggtcgatcg cgccgtgcgg
      661 ataggcggca tgaccggacc gcccgcggac gacgatgtcg aaccggtccg acgcggcgag
      721 gcaggcgccg tgcacgaagc cgaaatgccc caccgccatg tcgggatgat tgtggaagcc
      781 cagcgccatg tcgatcttgg gggacagcat cagcccgtcc gcgatcatgg cacgcatgcc
      841 gccgacgcct tcctcggccg gctggaacac cagcttcacc gtgccggcga gttgcggcgc
      901 aatctctttc agcacctcgg cgacgccgat cagcgtggtg gtgtggatgt cgtggccgca
      961 ggcgtgcatc aggccggggt tctggctggc gaagggcagg ccggtctcct cctggatcgg
     1021 caacgcgtcc atgtcggcgc ggatcgccag caccgggccg ggacgcccac cctcgatcag
     1081 ccccacaaca ccggtcttgc cgatgccggt ctggtgcgga atgcccaggc gggtgagctc
     1141 gcgcgcgacc acgccggcgg tgcggacctc ctcgaagccg agttcgggat gggcgtggat
     1201 gtcgcggcgg atctcgatca gctgcggctc gatgcgttcc gcgatgctgc ggatggcggt
     1261 ggtcggatcg ttgggaaggc tggaggacat gggcggcggc ctgttgcgaa tgaggcggcc
     1321 attgtgcgga ttgcgccctc ccaccgcaag aatgcgcgcc ttacgacgtc cagtgcgccg
     1381 gctgccagct ccagcccgac ggcgtctggg cccaataccc cggctggaac agcggaccgt
     1441 ggccggccgc cgggacatac tggcccttct gccagacata gcccgagccg ttccaatccc
     1501 agtggcccgg ctgccacatc aacgcctgcg cgctgaccgg cggcagcggc cggtgctcgg
     1561 ccagcggggc gggcaccggc ggatagggat tgccggtgct cgtggtttcc gtggcacagc
     1621 cggccagcag ggcggccagg accagcacaa caggtgcgaa gcggtatttc atcggccctc
     1681 cggctcggcc tgcaagcgaa acttcatgcg tgaaatggcc tcacgcccgt ccagtttcca
     1741 caacaaatcc gttaactcgg caacagaatg gtcgcgatcg cctgcgcggc aatgccctcg
     1801 ccgcggccgg tgaagcccag gcgctcggtc gtcgtcgctt tcaccgacac gcggccggga
     1861 tccaccccca gcagcgcggc aaggcgcgcc atcatcgccg cggcgtgcgg ggcgatcttg
     1921 ggccgctcgc agatcagcgt cacgtcggcg ttagccagca taccgccgcg cgcggcgatc
     1981 cgctctgccg cgtggcgaag gaaccgggcg ctgtccgcgt ccttccaggc ggcctggctg
     2041 ggcggaaaat ggcggccgat atcgccctcg gccaatgcgc catagatcgc gtcgcacagc
     2101 gcatggatac cgacatcggc atcggagtgg ccggccagcc cctgatcatg cggcacttcc
     2161 accccgcaca gcatcagctt gcggccaggc tccagcacgt ggacgtcgaa gccggtgccg
     2221 acgcgcggga tcagggtggg ggccatgatg cgctccagcc gagcgagatc ctcgggatag
     2281 gtcagcttga tgttgtcctc ggaaccggga acgatcgcca ctgtctcgcc ggcggcttcc
     2341 aacaaggacg catcgtccgt ggcgccggcg atgccggcac ggtgcgccgc gagcaggacc
     2401 ggaaagcgga aggcctgcgg cgtctgcgcg cggtacagcc cgtcgcgcgg cacggtttcc
     2461 atgatcacgc cgcggccgac gcgcttcagc gtgtcggcca ccggcaagcc agggattgcc
     2521 ccggctgcct gctccagcgc cgcgagcagg gccggcaccg tgcccggcgg aatgcagggc
     2581 cgcgcggcgt cgtgcaccag cacaatgtcc ggggcatgcg gcaccagggc ctc
//
LOCUS       DASSZH010000019         4747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1585600, whole genome shotgun sequence.
ACCESSION   DASSZH010000019 DASSZH010000000
VERSION     DASSZH010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585600"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VFN42_00330"
     CDS             complement(<1..687)
                     /locus_tag="VFN42_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HET8995089.1"
                     /translation="MLSVNDIIDRLGGIEAAARLTGIGTEAVRKWRQSGAIPSRHWPA
                     VIAATGLHLSDLQGAAPMPQADEAQPPSGATAALVLADGSVFWGRGFGAHTAAPAPVG
                     EVCFNTGMTGYQETLTDPSYAGQIITFTFPHIGNVGTNAEDIEATTIAARGLVVKQDV
                     TDPSNWRAAQHLDGWLRARGIAGVAGVDTRALTVRIRDGGAPSGVLAFPADGRFDLPA
                     LQAQAAAWPGL"
     gene            complement(780..2711)
                     /locus_tag="VFN42_00335"
     CDS             complement(780..2711)
                     /locus_tag="VFN42_00335"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET8995090.1"
                     /translation="MMENAWRRRWLFCLWSAALGIPLAGPAIAAPHDNDTATPIKHVI
                     VIYGENRSFDHLFATYVPPKGQTIRNLLSEGIIDKAGKPGPNFVKAMQYRATDSDAYS
                     IAPAKAGVYAMLPPPMTGSAHGAASDTDPPPFETLAAAQAADHSLPRDAVAELTTGAT
                     GLPRHSIDTRIADVNKLPPGPFPLTPGVPYDAYAASPVHRFYQNWQQSDCAPDHASTD
                     NPSGCLHDLYAWVEVSVGAGSNGKPQPAQFNDQSTGEGSTALGFYNVQQGDMPYFTKL
                     AEQYTIADNYHQPAMGGTGLNSIFAGFGDALWYTGADGEPGTPPSNQVENPNPQKGAN
                     NWYTQDGYHGGSYSDCADPKQPGVGAVVGYLQALPHKIDPHCAPNRYYLLNNYPPGYY
                     GNGSVNHGTFVVPPVTSRSIGDVLHDAGVSVTWFAQGWDRYRANPHDPHNTYCDICDP
                     FNYETAIMTKPALRDAMIQDIRNFNADLDRGVLPAVSLVKPGALDDGHPGSSKFGLFE
                     AFTRRIITALQAHPKLWQSTAVFITTDEAGGYYDSGYIQPLDFFGDGPRIPMIVVSPW
                     STGGRVVHSYTDHASVLKFIEANWHVGPISSRSRDNLPNPTPTKGDPYVPANSPAIGD
                     MMDMFEFGTPPASDSTPGK"
     gene            complement(2858..4213)
                     /locus_tag="VFN42_00340"
     CDS             complement(2858..4213)
                     /locus_tag="VFN42_00340"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8995091.1"
                     /translation="MPSGLRSDQKLTPEMRRAVFAGCIGFAVDFFDIYLPILALAPVI
                     RYFEPKGLSSSATTTIYFFTFAATLFGRPCGAVLFGHYADRIGRRRTTMISIAGFGAL
                     TFLIACLPGYASLGVWALVLLVVLRFVDGVFMGGEYTSNNTLALEMVPKDRRGFVGGV
                     LQGAFPIGFAMVSAVTSFMFWATTKQQYYAWGWRVTFIFGALMAFAFLLYYRTVPESP
                     MWEAAEKSESPLKEVFSGHHLRNLGQVFLMMLGFWTASQCASILPGIMIQHLHLTPEL
                     TSTAFLIGSVLLFFGFVGVGLLSQVIGRRPTIVLCGAAVLVAGTFLYYALVHHALVGG
                     SVAGTMLLVIAFDVLVVSPWGVVTTYISERFPTHVRASGYGIGYSAAVVIPAFAGFYL
                     LGLSHFMPYEFTPMVLLLLAGGLMMAGGLLGPETRDVELHLPDLGRSGESVAAGPLPA
                     E"
     gene            complement(4322..>4747)
                     /locus_tag="VFN42_00345"
     CDS             complement(4322..>4747)
                     /locus_tag="VFN42_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET8995092.1"
                     /translation="CDIRIATEGSRFGIPAARLGLGNGAAGVKKLMDIVGPSFTKEIF
                     FTARHFTAAEAQGMGLLNRVLPEAELEDYVRNYCATIADNAPLTMRALKRTVGELSKG
                     PQGDLAASEQLVKACFDTQDFIEGRRAFMEKRRPVFRGM"
BASE COUNT          828 a         1529 c         1587 g          803 t
ORIGIN      
        1 cagccccggc caggcggctg cctgggcctg caatgccgga agatcgaacc gcccgtccgc
       61 cggaaatgcc agcacgccgc tcggcgcccc gccgtcgcgg atgcgcacgg ttagcgcccg
      121 ggtgtccacc cccgccaccc cggcgatgcc gcgcgcccgc agccagccgt ccaggtgctg
      181 ggcggcgcgc cagttggacg ggtcggtgac gtcctgcttc accacaaggc cacgggcagc
      241 gatggtggtc gcctcgatat cctcggcatt ggtgccgaca ttgccgatat gcgggaaggt
      301 gaaggtgatg atctgcccgg cataggacgg gtcggtcagg gtctcctggt agccggtcat
      361 gccggtattg aagcagacct cgcccaccgg cgcgggcgcg gcggtgtgcg cgccgaagcc
      421 gcggccccag aacaccgatc cgtccgccag caccagcgcg gcggtggcgc cggagggcgg
      481 ctgcgcttcg tcggcttgcg gcatgggcgc ggctccctga aggtcggaca ggtgcaggcc
      541 ggtggcggcg atcaccgcgg gccagtggcg ggacgggatg gcaccggact ggcgccattt
      601 gcgcaccgcc tcggtgccga ttccggtcag ccgagcggcc gcctcgatgc caccgagccg
      661 gtcgatgatg tcgttgacgc tgagcatgca gcgtatctat gggaaataac ttcccatcgc
      721 aatgggtcgc cccggcctgg gaggaaattt cctcccgggc cgggccgggc cgggcaggcc
      781 tatttgcccg gtgtgctgtc gcttgccggg ggtgtcccga attcgaacat gtccatcatg
      841 tcgccgatcg ccgggctgtt cgccggcaca taggggtcgc ccttggtggg cgtggggttg
      901 ggcaggttgt cccggctgcg actggagatc ggcccgacat gccagtttgc ctcgatgaat
      961 ttcagcaccg aggcatggtc cgtatagctg tgcaccacgc gcccgccggt cgaccagggc
     1021 gataccacga tcatcggaat gcgcggcccg tcgccgaaga agtccagcgg ctggatgtag
     1081 cccgaatcat aatagccgcc cgcctcgtcc gtggtgatga acaccgccgt ggactgccac
     1141 agcttgggat gtgcctgcag cgcggtgatg atccgcctgg tgaaagcctc gaacagaccg
     1201 aatttcgatg accctggatg gccatcgtcc agcgcacccg gcttcaccag ggacaccgcc
     1261 ggcaacacgc cgcggtccag gtcggcgttg aaattgcgga tgtcctggat catcgcatcc
     1321 cgcagcgcgg gcttcgtcat gatggcggtt tcgtagttga acggatcgca gatgtcgcaa
     1381 taggtgttgt gcggatcgtg cgggttcgcc ctgtagcggt cccagccctg agcgaaccag
     1441 gtgaccgata cgccggcatc gtgcagcacg tcgccgatgc tgcggctggt gaccggcggg
     1501 accacgaagg tgccgtggtt cacgctgcca ttgccgtagt accccggcgg ataattgttc
     1561 agcaggtagt agcggttcgg cgcgcaatgc gggtcgatct tgtgcggcag cgcctgcaga
     1621 taccccacca ccgcaccgac accgggctgc ttcggatcgg cgcagtcgct gtacgagccg
     1681 ccatggtagc cgtcctgggt gtaccagttg ttggcgccct tctgcggatt cggattctcg
     1741 acctggttgc tcgggggcgt tcccggctct ccgtcggccc ccgtgtacca cagcgcatcc
     1801 ccgaacccgg caaagatgct gttcaggccg gtgccgccca tcgccggctg atggtaattg
     1861 tcggcgatgg tgtattgttc ggcgagcttg gtgaaatacg gcatgtcgcc ctgctggaca
     1921 ttgtagaagc ccagggcggt tgagccttca ccggtgctct ggtcattgaa ctgtgctggc
     1981 tgcggcttgc cattgctgcc ggcgccgacc gagacctcca cccaggcata caggtcatgc
     2041 aggcagccgc tggggttgtc cgtgctggca tgatccggcg cacaatccga ctgctgccag
     2101 ttctggtaga accggtgcac cggactggcg gcataggcgt cgtacggcac gcccggagtc
     2161 agcgggaagg ggcccggcgg cagcttgttc acatcggcaa tgcgcgtgtc gatgctgtgc
     2221 cgcggcaggc cggtggcgcc ggtggtcagt tccgccacgg catcccgcgg caggctgtga
     2281 tccgccgcct gagccgcggc cagcgtttcg aaggggggcg gatcggtgtc gcttgcggcg
     2341 ccgtgggcac tgccggtcat tggcggcggc aacatggcgt acacgcccgc ctttgccgga
     2401 gcaatgctgt aggcatcgct gtcggttgcc cggtattgca tcgccttgac gaagttcggc
     2461 ccaggtttgc cggctttgtc gataatgcct tccgagagca gattgcggat cgtctgcccc
     2521 ttgggcggga cataagtcgc gaataaatgg tcgaagctgc ggttctcgcc gtagatgacg
     2581 atgacatgct tgataggggt cgcggtgtca ttgtcatgcg gcgcggcgat ggccggaccg
     2641 gcaagcggga ttccaagggc ggcactccat aagcaaaaca accacctgcg ccgccaggcg
     2701 ttttccatca ttccccgctc cgtctggttt gcttccgcgt cgggcggggc cctaacaccg
     2761 gctcgtgaca attcgtgggc agcaaactga cggcggggtg acgggccgcc gccgcggcac
     2821 cgaccaacca accgcggcgg cggccatcag ggcccgacta ctccgccggc aacgggccag
     2881 ccgccacgga ttcgccggac cggccgagat ccggcagatg cagttccacg tcgcgcgtct
     2941 ccggccccag caagccgccg gccatcatca gcccgcctgc cagcagcagc agcaccatcg
     3001 gggtgaattc gtacggcatg aagtgcgaca ggcccagcag gtagaacccg gcgaaggccg
     3061 ggatcaccac ggcggcgctg tagccgatgc cgtagccact ggcgcgcaca tgcgtgggaa
     3121 agcgctccga gatgtaggtg gtgaccacgc cccagggcga taccaccagc acgtcaaagg
     3181 caatcaccag cagcatggta cccgccaccg aaccgccgac cagggcatgg tgcaccagcg
     3241 cgtagtacag gaacgtgccc gccaccagca cggccgcgcc gcacagcacg atggtgggac
     3301 ggcggccgat cacctggctc agcagcccta cgccgacgaa gccgaagaac agcagcacgc
     3361 tgccgatcag gaaggcggtg gaggtcagtt cgggcgtcag atgcagatgc tggatcatga
     3421 tgcccggcag gatcgacgcg cattgcgagg cggtccagaa tcccagcatc atcaggaaga
     3481 cttggccgag gttgcgcagg tggtggccgg agaacacttc cttcagcggc gactcgctct
     3541 tctccgccgc ctcccacatc ggcgattccg gtacggtgcg atagtacagc aggaaggcga
     3601 aggccatcag cgcgccgaag atgaacgtca cccgccagcc ccaggcatag tattgctgct
     3661 tggtggttgc ccagaacatg aagctggtga cggcggagac catggcgaag ccgatcggaa
     3721 aggcgccctg cagcacgccg cccacgaaac cgcggcggtc tttcggcacc atttccagcg
     3781 ccagcgtgtt gttggacgtg tattcgccgc ccatgaacac gccatcgacg aaacgcagca
     3841 ccaccagcag caccagcgcc cagacgccca ggctggcata acccggcagg caggcgatca
     3901 ggaaggtgag cgcgccgaac ccggcgatgc tgatcatggt ggtacggcgg cggccgatgc
     3961 ggtcggcata gtggccgaac agcaccgcac cgcagggccg gccgaacagc gtcgccgcga
     4021 aggtgaagaa gtagatggtg gtggtggccg acgaactgag ccccttcggc tcgaagtagc
     4081 ggatcaccgg ggcaagcgcg aggatcggca gatagatgtc gaagaaatcg acggcaaatc
     4141 cgatgcagcc tgcgaacacg gcccgtcgca tctcgggcgt aagtttctgg tcgctgcgca
     4201 aaccagacgg cattggccga tcctcccgtt cttgctattt gacgattgtg ctcagcttag
     4261 cactggcgtc tttgcctcgc aaattgcaaa accgggagcg cgcgaagtcg cggccctggg
     4321 actacatccc acgaaatacc gggcggcgtt tctccatgaa ggcacgccgg ccttcgatga
     4381 aatcctgcgt gtcgaaacag gccttgacca gttgctccga tgccgccagg tcgccttgcg
     4441 gtcccttcga caattcgccg accgtgcgct tcagcgcacg catggtcagc ggcgcattgt
     4501 cggcgatggt ggcgcaatag ttccgcacgt agtcttccag ctctgcttcc ggcagtaccc
     4561 ggttcagcag gcccatgccc tgcgcttccg ccgcggtgaa atggcgcgcg gtgaagaaga
     4621 tctccttggt gaaggacggg ccgacaatgt ccatcagctt cttcaccccg gccgccccat
     4681 tgccgagacc gagccgcgcg gcgggaatgc cgaacctgga cccttccgtg gcaatgcgga
     4741 tgtcaca
//
LOCUS       DASSZH010000020         2402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_119710, whole genome shotgun sequence.
ACCESSION   DASSZH010000020 DASSZH010000000
VERSION     DASSZH010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119710"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..956)
                     /locus_tag="VFN42_00350"
     CDS             complement(132..956)
                     /locus_tag="VFN42_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET8995093.1"
                     /translation="MHDLPRVVLRGGAQIGEKDQPAASCYLVERGLVASYVNVDDDRR
                     TCVGLLGPGDVVGLGTLFGRSGDECARSQAIVPVQLLCIPAQSLRLMLNRSPALLDVC
                     LQQLQASLTEIQHVAACNARHLLPARCAHWLLRLQARVGDVLPVTHEFLASVLGVRRA
                     GITVTLQALQRNGAIRQQRGSVVIIDPEQLRGTACGCPVAIGHPPFVMPASQVAAPAG
                     ALGQASGERVSPRALSDSAIRARMTEADSVNPATARIEAVLQVCRAVMERTHEMLV"
     gene            1348..>2402
                     /locus_tag="VFN42_00355"
     CDS             1348..>2402
                     /locus_tag="VFN42_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HET8995094.1"
                     /translation="MAERENLLRHQTSPYLLQHADNPVHWRPWGAAALAEAVAEDKPI
                     LLSVGYAACHWCHVMAHESFENPQVAALMNTLFVNIKVDREERPDIDHLYMSALHALG
                     EQGGWPLTMFLTPQGEPFWGGTYFPPEPRWGRPSFQQVLQGVASAYRERDQAIMQNSG
                     ALRRYLERAAAAEPGDLPTPVHLDAVAGAYLRMNDPEQGGLKGAPKFPNPPIFRLLWQ
                     NAFRTGQPEGQDALHLMLQRMSQGGIYDHLGGGYARYATDAIWLVPHFEKMLYDNAQL
                     LELLAFAHAHRPDPLYAQRAAETVGWLMRDMTAAAVDGRAGFAASEDADSEGEEGKFY
                     VWTEPEIDRLLGARAAA"
BASE COUNT          411 a          803 c          792 g          396 t
ORIGIN      
        1 gagttcggca atgcgatggt ccatgtcctc cagggccacc cgcgcctgct ccagttcgct
       61 ccgcagccgc tcgatctccc cgctgtcagg gggccgtcga atgtcgtcgc ttgctcgatc
      121 catgtctctg atcacaccag catttcatga gttcgttcca ttacggcgcg acagacctgc
      181 agcacggcct cgatgcgggc cgttgccgga tttacggagt cggcctcggt catccgcgcc
      241 cggatggcgg aatccgacaa ggcgcgggga ctgacgcgct cgcccgaggc ctgccccaac
      301 gcgccggcag gcgccgccac ctggctggcc ggcatgacga agggcggatg cccgatggcg
      361 accgggcagc cgcatgccgt gccgcgcagt tgctccgggt cgatgatgac gacactgccg
      421 cgctgctggc gaattgcgcc gttccgctgc aacgcctgca gcgtgaccgt gatgccggct
      481 cggcggacac ccaggaccga cgccaggaat tcatgcgtca ccggcagcac gtcgccgacc
      541 cgcgcctgga ggcgcagcag ccagtgggca cagcgcgcgg gcagcagatg gcgggcattg
      601 caggcggcga cgtgctggat ttcggtcagg ctcgcctgaa gctgctgcag acagacgtcc
      661 agcaatgcgg gcgagcggtt cagcatcagg cgcaggctct gcgccgggat acacagaagc
      721 tggacgggaa cgatggcctg gctccgggcg cactcgtcgc ccgatcggcc gaacagcgtg
      781 ccgagtccga ccacgtcgcc aggtcccagc agcccgacac aggtccgccg gtcatcatcc
      841 acattgacgt aactcgccac cagtccgcgt tccaccaggt agcaggacgc ggcgggttgg
      901 tccttttcgc cgatctgtgc gcccccgcgc agaacaaccc gtggcagatc atgcatagca
      961 aacgactcga tcccgctata gttcgcattg gtccaaatag gcggtatcgg cgaaagtccg
     1021 gctgcattta ttgacatgag cgatgaagcc tccccaggct caaaccatgt ggacattaca
     1081 tcctctggta ccgggatgtc gatgcaagcg ggcctattct ggttcacgaa tagccgaacg
     1141 gcgctccagg gggcggccgt gccgctcaaa cggtcagaaa tcatgtgcca gccttcatca
     1201 tagggacacc tgccggcaga atgattgacg aaaggcaagc ggtgggcctg tgactcccgc
     1261 ggaccgtgac ttcaatgtca tgcgccgggt gtgtttgcgg ccagtgcttc ccatctttcc
     1321 gccaactgca ttcaaggacg cgccgtgatg gccgaacgcg agaacctgct gcgccaccag
     1381 acttcgccct atctgctgca gcacgcggac aacccggtgc attggcgtcc gtggggcgcg
     1441 gcggcgctgg cggaggccgt ggccgaggac aagcccatcc tgctctcggt cggttatgcg
     1501 gcgtgtcact ggtgccacgt gatggcccat gaatcgttcg agaacccgca ggtcgccgcg
     1561 ctgatgaaca cgctgttcgt caacatcaag gtggatcggg aggagcgtcc ggatatcgac
     1621 cacctgtaca tgtccgcgct gcacgcgctg ggcgagcagg gcggctggcc gctgaccatg
     1681 ttcctgaccc cgcagggcga gccgttctgg ggcggcacct atttcccgcc ggagccgcgc
     1741 tggggccgcc cgtccttcca gcaggtgcta caaggcgttg cgtccgccta tcgcgagcgc
     1801 gaccaggcca tcatgcagaa cagcggggcg ctgcgccgct atctggagcg tgccgccgcg
     1861 gcggagccgg gcgacttgcc gacaccggtc catctggatg cggtggccgg cgcctatctg
     1921 cgcatgaacg acccggagca gggcgggctg aagggcgcac cgaaattccc caacccgccg
     1981 atcttccgcc tgctctggca gaacgcgttc cgtaccgggc agccggaggg ccaggacgcc
     2041 ttgcacctga tgctgcagcg catgtcgcag ggcggcatct acgatcacct tggcggcggc
     2101 tatgcacgct acgcgaccga tgcgatctgg ctggtgccgc atttcgagaa gatgctgtac
     2161 gacaatgcgc agttgctcga attgctggcc ttcgcccacg cccaccggcc ggacccgctc
     2221 tatgcgcagc gggccgccga gacggtgggc tggctgatgc gcgacatgac cgcggcggcg
     2281 gtggacggac gcgccggctt cgccgcctcg gaagatgccg acagcgaagg cgaggaaggc
     2341 aagttctacg tctggaccga accggagatc gaccgcctgc tcggcgcccg cgccgcggcc
     2401 tt
//
LOCUS       DASSZH010000021         2624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_596952, whole genome shotgun sequence.
ACCESSION   DASSZH010000021 DASSZH010000000
VERSION     DASSZH010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596952"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1612
                     /gene="glyS"
                     /locus_tag="VFN42_00360"
     CDS             <1..1612
                     /gene="glyS"
                     /locus_tag="VFN42_00360"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003629727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HET8995095.1"
                     /translation="DGAVVPFALREGADDGHGLATGDQTEGHRFLAPGAFPVSSAADW
                     ADKLRSHRVLPEADERKRVIAEGIHALAAERQLSVVDDPGLLDEVSGLVEWPVPLLGR
                     IAQEHMDLPPEVMQVSMRVNQRYFALRTADGRAAPYFAFVANVEAPDGGAAIVAGNER
                     VLRARFADARHFWDLDRKAPLESRVPLLDDITFHAKLGSQGDRVRRLKQLAEHLAHQV
                     HAAPAMAQRAAALAKADLVTGMVGEFPELQGVMGRYYALHDGEPHEVADAIRDHYAPK
                     GPADAVPSAPVSIAVALADKLDQLAGFFAIGEKPTGSGDPYALRRAALGVIRIIRENG
                     LRLKLLPLIEEAGRNVHRHGVEPATAQDILDFLAERLRVQLRGDGARHDVLAAVFAAK
                     GEQGADDDVVRLLARTDAVAALLGSEDGANLLTAYRRAANILRIEERKDGPHDAAPEQ
                     TLLREPAELDLNHALQGLPMVLRLLDQEDFTGAMAGLAGLRAPLDRFFDAVTVNAPEP
                     ELRRNRLRLLNQVRVTMDRVADFSRIEG"
     gene            1626..>2624
                     /locus_tag="VFN42_00365"
     CDS             1626..>2624
                     /locus_tag="VFN42_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET8995096.1"
                     /translation="MTKWVYSFGAGHNEGRADMKNLLGGKGANLAEMASIGLPVPPGF
                     TITTEVCTAFYKNNRNYPAELEDEVRKALTQVEQALGRKFGDAKNPLLVSVRSGARVS
                     MPGMMDTVLNLGLNDETVQGLAAMAGDERFAWDSYRRFIQMYGSVVLGVDHHRFEEII
                     EHAKLETGAVEDTQLKAEDWQRVATAYKEMIKQDTGKPFPLKPQEQLWGAVGAVFGSW
                     MNPRAITYRRLHDIPAEWGTAVNVQAMVFGNMGNDCATGVCFTRDPSTGENRFYGEYL
                     INAQGEDVVAGIRTPQPVSSARAKPDETPMEAVMPEAYRELLAVREKLEQHYRDMQD"
BASE COUNT          439 a          894 c          901 g          390 t
ORIGIN      
        1 ggacggcgcc gtggtgccct tcgcgttgcg tgagggcgcc gatgacgggc acggcctggc
       61 caccggtgac cagaccgagg gccaccgctt cctcgccccc ggcgccttcc ccgtcagctc
      121 cgccgcggac tgggctgaca agctgcgttc ccaccgcgtg ctgccggaag ccgatgaacg
      181 caagcgggta atcgccgagg gcatccacgc cctcgccgcc gaacgccaac tgagcgtggt
      241 ggacgacccc ggcctgctcg acgaagtgtc cggactggtc gaatggcccg tccccctgct
      301 cggccgcatc gcgcaggagc acatggacct gccgccggag gtgatgcagg tctccatgcg
      361 ggtgaaccag cgctacttcg cgctgcgcac cgcggacggt cgggccgcgc cgtatttcgc
      421 cttcgtcgcc aatgtggagg cgccggacgg cggcgccgcg atcgtcgccg gcaacgagcg
      481 cgtgctgcgc gcccgcttcg ccgacgcccg ccacttctgg gatctggacc gcaaggcgcc
      541 gctggaatcc cgtgtgccgc tgctggacga catcaccttc cacgccaagc tgggcagcca
      601 aggcgaccgg gtgcgccgcc tgaagcaact ggccgaacac ctcgcccacc aagtgcacgc
      661 cgcgcccgcg atggcacaac gcgcggcggc cctggcgaag gccgacctgg tcaccggcat
      721 ggtcggagaa tttcctgaac ttcaaggtgt tatggggcga tattacgccc tgcacgatgg
      781 cgagccgcat gaggtggcgg atgccatccg cgaccactac gcgcccaagg ggccggcgga
      841 cgcggtgccg tccgccccgg tctccatcgc cgtggcgctg gccgacaagc tggaccagct
      901 cgccggcttc ttcgccatcg gcgagaagcc gaccggctcc ggcgacccct atgcgctgcg
      961 gcgggcggcg ctgggggtga tccgcatcat ccgggagaac ggcttacggc tgaaacttct
     1021 gccgctgatt gaggaagccg gccgcaacgt ccaccgtcac ggcgtcgagc ccgccacggc
     1081 gcaggacatc ctggactttc tggccgagcg cctgcgcgtg cagctgcgcg gggatggtgc
     1141 gcggcacgac gtgctggccg cggtgttcgc cgccaagggg gagcagggcg cggacgacga
     1201 cgtcgtccgg ctgctggccc gcaccgacgc tgtggccgcc ctgctcggct cggaggacgg
     1261 tgccaacctg ctcaccgcct atcggcgcgc cgccaacatc ctgcggatcg aggagcgcaa
     1321 ggacggcccg cacgatgccg caccggagca aaccttgttg cgcgagccgg ccgagcttga
     1381 cctgaatcat gccctgcagg ggctgccgat ggtattgcgc ctgctcgatc aagaggattt
     1441 caccggggcg atggctggtc tggcggggct gcgcgcgcca ttggacaggt tcttcgacgc
     1501 cgtgaccgtc aacgccccgg aaccggagct gcggcgtaac cgtttgcgtc tgcttaacca
     1561 ggttcgggtt accatggacc gcgtcgccga tttctcccgt atcgaaggtt agaccgaggg
     1621 ttcacatgac caagtgggtg tacagcttcg gagccggcca caacgagggc cgcgccgaca
     1681 tgaagaatct gcttggcggc aagggcgcca acctggcgga gatggcctcc atcggcctgc
     1741 ctgtgccgcc cggcttcacc atcaccacgg aagtctgcac cgcgttctac aagaacaacc
     1801 ggaactatcc ggccgagctg gaagacgagg tgcgcaaggc gctcacccag gtcgagcagg
     1861 cgctcggccg caagttcggc gatgcgaaga acccgctgct ggtgtcggtg cgctccggcg
     1921 cgcgcgtgtc catgccgggc atgatggaca ccgtgctcaa cctcgggctg aacgacgaga
     1981 ccgtgcaggg ccttgccgcc atggcagggg atgagcggtt cgcctgggat tcctatcgcc
     2041 gcttcatcca gatgtacggc tccgtcgtgc tgggcgtgga tcaccaccgg ttcgaggaga
     2101 tcatcgaaca cgccaagctg gagaccggcg cggtcgagga cacccagctg aaggcggaag
     2161 actggcagcg cgtcgccacc gcctacaagg agatgatcaa gcaggacacc ggcaagccgt
     2221 tcccgctgaa gccgcaggag cagctgtggg gcgcggtcgg cgcggtgttc ggttcctgga
     2281 tgaacccgcg cgcgatcacc tatcgccggc tgcacgacat ccccgccgaa tggggcaccg
     2341 cggtcaacgt gcaggcgatg gtgttcggca acatgggcaa tgactgcgcc accggcgtgt
     2401 gcttcacccg cgacccgtcc accggcgaga accgtttcta cggcgagtac ctgatcaacg
     2461 cgcagggtga ggacgtggtg gccggcatcc gcacgccgca gccggtgtct tccgcgcgcg
     2521 ccaaaccgga tgagacgccg atggaagcgg tgatgccgga ggcataccgc gaactgctgg
     2581 cagtgcggga gaagctggaa cagcactacc gcgacatgca ggac
//
LOCUS       DASSZH010000022         4804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1091338, whole genome shotgun sequence.
ACCESSION   DASSZH010000022 DASSZH010000000
VERSION     DASSZH010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091338"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1536)
                     /gene="yidC"
                     /locus_tag="VFN42_00370"
     CDS             complement(<1..1536)
                     /gene="yidC"
                     /locus_tag="VFN42_00370"
                     /inference="COORDINATES: protein motif:HMM:NF002353.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET8995097.1"
                     /translation="MDQKRLFMAIAISLAILVGFEYLIGPYLPHPPPPKEQTTALNGS
                     EPGKPAATAQNAVPSAPGSVAAATLNAQPKNVPRVQIDAPSVRGSISLLGARLDDLIL
                     THYRETLKPDSPDVKLLSPPGTPHPDYVQYGWTAAPGETVKVPGNNTLWTASAKTLTP
                     SHPVTLSWDNGQGLTYQLILSVDDRYMFTVKQAVKNTSGQPVKLFPWKRVRRDYEPPP
                     SGEYILFVGMLGVVDNTLKETTYANAQSEAKDNHGIAYTATAPGGWAGFTDKYWLTAM
                     IPDQSVPETVTFHNLKENGTEHYQVDYITKQPETIAPGAESALPSRLFTGAKVVRLLD
                     AYGTQYHIPSFDKAVDWGWFYFLTKPIFYCLDWLNALVGNFGIAIMLFTVGVKALFFP
                     LANYSYRSMSKMKLLGPKMTALREKYKDDPQKMQSEMMAMYKAEKVNPASGCLPMVVQ
                     IPVFFSLYKVIFVTIEMRQAPFFGWIHDLSAIDPTNVFRLFGLLPWHPGDISPFLHLG
                     AWPL"
     gene            complement(1672..1890)
                     /gene="yidD"
                     /locus_tag="VFN42_00375"
                     /pseudo
     CDS             complement(1672..1890)
                     /gene="yidD"
                     /locus_tag="VFN42_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423334.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
     gene            complement(1887..2276)
                     /gene="rnpA"
                     /locus_tag="VFN42_00380"
     CDS             complement(1887..2276)
                     /gene="rnpA"
                     /locus_tag="VFN42_00380"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET8995098.1"
                     /translation="MHAEPLLERRDAPGRPERRKPGRLIRRAEFLRVAAKGRKVAMPG
                     LVLQALPRSDAGPARLGFTVTKKVGNAVIRNRTRRRMKEAARLLLRAQAVQGVDLVLI
                     GRDGTRGRDFVALQEDLRRALARAGVT"
     gene            complement(2303..2437)
                     /gene="rpmH"
                     /locus_tag="VFN42_00385"
     CDS             complement(2303..2437)
                     /gene="rpmH"
                     /locus_tag="VFN42_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET8995099.1"
                     /translation="MKRTYQPSKLVRKRRHGFRARMATVGGRKVLANRRAKGRKRLSA
                     "
     gene            2671..3369
                     /locus_tag="VFN42_00390"
     CDS             2671..3369
                     /locus_tag="VFN42_00390"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET8995100.1"
                     /translation="MRILRPLLPLLVLLAVLAPVWFSGVGHDFSWHGLARHQAALTGW
                     VAAHPVIAPLAYVGFYVAVAALCLPEAAVVTVAGGLLFGTLFGGALAVIGATLGSIVL
                     FLVARSALAGSFVRGGGALLARTRAALQRDGFAYLLAIRLIPAFPFWLVNLAAAVGGM
                     RLLPYATATFIGIIPVTMVFSSLGEGLGKVLAQGREPDVLVVFSPSVLLPLLALAALS
                     LVPVLWRHWKARRG"
     gene            3362..4765
                     /locus_tag="VFN42_00395"
     CDS             3362..4765
                     /locus_tag="VFN42_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8995101.1"
                     /translation="MAEFDLAVIGAGAAGLSVTAVAAQLGLRVALIERDRMGGDCLNV
                     GCVPSKALLAASHAATAARGAGRFGVRLGDPVIDWAAVQAHVHGAIATLEPADSETRF
                     RGLGASVLRGEARFAAPDALLVDGRRITARRIVIAAGSRAAVPPIPGLDAVPFLTNAS
                     LFDLPERPEHLLILGGGPIGLEMADAFAGLGVRVTLVEAAHIAGKEDPELVAGLRSAL
                     AGRGVVLLENARVAAVEAGPTLVLADGRRVAGSHLLVAVGRRPNLEALDLEAGGVRAS
                     PAGVVTDRGLRSPSNRRVFAVGDIADPAGIGPRAFTHVGSYHAGIVIRRALFRLPARV
                     DYAALPRVTYTRPELAQVGLTEAEAAGRGVAVLRWNLAENDRAVAEGDTAGLVKLVVA
                     RGRVIGAGILAPGAGEMIGLWALAIASRTPVSRLAGLVLPYPTHSEAGKRAAGTAYEP
                     RLFAPMTRRVVGWLQRL"
BASE COUNT          623 a         1619 c         1748 g          814 t
ORIGIN      
        1 cagcggccac gcccccagat gcaggaatgg cgagatatcc ccgggatgcc agggaagcag
       61 cccgaacagc cggaacacgt tggtcggatc gattgccgac aggtcgtgga tccagccaaa
      121 gaacggcgcc tggcgcatct cgatggtgac gaagatcacc ttgtacagcg agaagaacac
      181 cggaatctgc accaccatgg gcaggcagcc cgaggcggga ttcaccttct ccgccttgta
      241 catcgccatc atctcggact gcatcttctg cgggtcgtcc ttgtacttct cccgcagcgc
      301 cgtcattttc ggcccgagca gcttcatctt gctcatggag cggtaggagt agttcgccag
      361 cgggaagaac agcgccttca cgccgacggt gaacagcatg atggcgatgc cgaaattgcc
      421 gaccagcgcg ttgagccagt ccaggcaata gaagatcggc ttggtcagga agtagaacca
      481 gccccagtcc accgccttgt cgaaggacgg gatgtggtac tgcgtgccgt acgcatccag
      541 cagccgcacc accttggcgc cggtgaacag tctggacggc agcgcggatt cggcacccgg
      601 cgcgatcgtt tccggctgct tggtgatgta gtccacctgg tagtgttcgg tgccgttttc
      661 cttcaggttg tggaaggtca ccgtctccgg caccgactgg tccgggatca ttgcggtcag
      721 ccagtatttg tcggtgaaac ccgcccaacc gccaggcgcc gtcgcggtat aggcaatgcc
      781 gtggttgtcc ttcgcctcgc tctgcgcgtt ggcgtaggta gtctccttca gcgtgttgtc
      841 gaccacgccg agcatgccga cgaacaggat atattcgccg gacggcggcg gctcgtagtc
      901 gcggcggacc cgcttccagg gaaacagttt caccggctgg ccgctggtgt tcttcaccgc
      961 ctgcttgacg gtgaacatgt agcggtcgtc caccgacagg atcagttgat aggtcaggcc
     1021 ctggccgttg tcccaggaca gcgtcaccgg atgtgacggc gtcagcgtct tggcggacgc
     1081 ggtccatagc gtgttgttgc cgggaacctt gacggtctcg ccgggcgccg cggtccagcc
     1141 gtactgcaca taatccgggt gcggcgtgcc gggcggcgac agcagtttca cgtccggcga
     1201 atccggcttc agcgtctcgc ggtagtgcgt caggatcagg tcatccagcc gggcgccaag
     1261 caggctgatc gagccgcgca cgctcggggc atcgatctgc acccgcggca cgttcttcgg
     1321 ctgcgcgttc agggtggctg cggcaaccga accaggcgcg gaaggcacgg cattctgcgc
     1381 cgtggcggcc ggctttcccg gctcggatcc gttcagcgcg gtggtctgtt ccttgggcgg
     1441 cggcggatgg ggcaggtacg gcccgatcag gtattcgaac ccgaccagga tcgcgagcga
     1501 gatcgcgatc gccatgaaca gccgcttttg gtccatttag taactcttcc cgtagtcagt
     1561 cccgccggtt gctccggcgg ggtgaggcgc atgcggccga gccgtcttct gctgccccgc
     1621 ccgcaagggg agggggagtt tcgtgtcgtc gtctgcgctg cactgcagac gcggcggtac
     1681 cgggtcgtag ccgcagcggc accaggggtt gcagcgcagg atccgccagc cggccagcgc
     1741 gccgccgcgc aatgcgccat gctcgcgaaa cgcctcgatc gcgtaatcgc tgcagcttgg
     1801 atagaaccgg caattggcgc cgatcagcgg gcgcaaggtc cattggtagg cgcgcaccgc
     1861 gcccacggcg acaaaggcgg ccgggctcat gtcacgcctg cgcgggccag cgcccgccgc
     1921 agatcctctt gcagggcgac aaaatcgcgg ccgcgcgtgc cgtcgcgtcc gatcagcacc
     1981 aggtccacgc cctgcaccgc ctgcgcgcgc agcagcagcc gggcggcttc cttcatgcgg
     2041 cggcgggtgc ggttgcggat cacggcgttg ccgaccttct tcgtcacggt aaacccgagc
     2101 ctggccggac cggcatcgct gcgcggcagc gcctgcagca ccagcccggg catcgccact
     2161 ttgcggccct tggcggcaac acgcaggaac tcggcccggc ggatcagccg tcccggcttg
     2221 cgccgttccg gccggcctgg cgcgtcccgg cgctccagca agggttcggc atgcatcgac
     2281 tcaacggtcg ccgcgccggc ccttaggccg acaggcgctt gcgtcccttg gcacggcggt
     2341 tcgccagcac cttgcggccg ccgacggtgg ccatgcgggc gcggaacccg tgccggcgct
     2401 tgcggaccag cttcgacggt tgataggtgc gcttcacgag ccagttctcc ggaatctgtg
     2461 cgtccgcgcc aacgcggaca aggccccaca agggggccgc caaggcggcg gcttataggg
     2521 gggcgtgccc ggcccgtcaa tcgcgccgct ggcctttggc cggccttgcc ccgcacccgc
     2581 tattctgcca ccagcctcca ggcgatcgaa cggcagcgcg cggctggcgt tcccggtccc
     2641 accggtcctc ccagccgact cccctcctgc atgcgcatcc tgcgtcccct gctaccgctg
     2701 ctcgttctgc tggccgtgct ggccccggtc tggttcagcg gggtcgggca cgatttctcc
     2761 tggcacggcc tggcgcgcca ccaggccgcg ctgaccggct gggtggcggc gcatccggtc
     2821 atcgcgccgc tggcctatgt cggattctac gtggccgttg ccgcgctgtg cctgccggag
     2881 gccgcggtcg tcaccgtggc cggcgggctg ctgttcggca ccctgttcgg cggcgccctc
     2941 gccgtcatcg gtgcgacgct cggctccatc gtgctgttcc tggtggcgcg gagcgcgctg
     3001 gcaggcagct tcgtgcgcgg cggcggcgcg ctgctggcac gcacccgcgc cgccttgcag
     3061 cgcgacggct tcgcctacct cctggcgatc cggctgatcc cggccttccc cttctggctg
     3121 gtcaacctgg ccgccgcggt gggcggcatg cggctgctgc cctacgcgac ggcgactttc
     3181 atcggcatca tcccggtgac tatggtgttc agctcgctgg gggaggggct tggcaaggtg
     3241 ctggcgcagg gacgcgaacc ggacgtgctg gtggtgttct cgccgtccgt cctgctgccg
     3301 ctgctggccc ttgccgcgct ctcgctggtg cccgtgctgt ggcggcactg gaaggcgcgg
     3361 cgtggctgag ttcgacctcg cggtgatcgg cgccggtgcg gccgggctgt cggttaccgc
     3421 ggtggcggcg caactgggct tgcgggtggc gctgatcgag cgcgaccgca tgggcggcga
     3481 ctgcctcaat gtcggctgcg ttccgagcaa ggcgctgctg gcggcgtcgc atgcggcgac
     3541 ggcggcgcgg ggggcagggc ggttcggcgt gcggctgggg gacccggtga tcgactgggc
     3601 ggcggtgcag gcgcatgtgc acggcgccat cgccacgctg gagccggcgg attccgagac
     3661 gcggttccgc ggcctgggcg ccagcgtgct gcgcggcgag gcgcggttcg ccgcgccgga
     3721 cgcattgctg gtggacgggc ggcggatcac cgcgcggcgc atcgtgatcg ccgccggcag
     3781 ccgcgcggcg gtgccgccga tcccggggct ggatgcggtg ccgttcctga ccaatgcgtc
     3841 cctgttcgac ctgccggagc ggccggaaca cctgctgatc ctgggcggcg gcccgatcgg
     3901 gctggagatg gcagacgcct tcgccggtct cggcgtgcgc gtcaccctgg tggaagcggc
     3961 gcatatcgcc ggcaaggagg accccgagct ggtcgccggc ctgcgctctg cgctcgccgg
     4021 ccggggcgtg gtgctgctgg agaatgcccg cgtcgcggcg gtggaggcgg ggccgaccct
     4081 ggtcctggcg gatggccgcc gcgtcgccgg cagccatttg ctggtcgccg tcggccggcg
     4141 gccgaatctg gaggcgctgg acctggaggc gggcggcgtg cgggcatcgc cggccggggt
     4201 ggttaccgac cgggggctgc gctcgccgag caaccggcgc gtattcgcgg tgggcgacat
     4261 tgccgatcct gccgggatcg gaccgcgcgc cttcacccat gtcgggtcct atcacgccgg
     4321 catcgtcatc cgccgggcgc tgttccgcct gccggcgcgg gtggattacg ccgccctgcc
     4381 gcgggtcacc tacacccggc cggaactggc ccaggtgggg ctgaccgagg cggaggcggc
     4441 tggccggggc gtagccgtgc tgcgctggaa cctggcggag aacgaccggg cggtcgccga
     4501 gggcgacacg gcaggcctgg tcaagctggt ggtcgcacgc ggccgggtga tcggtgcggg
     4561 tatcctggcg cccggtgccg gcgagatgat cggcttgtgg gcgttggcaa tcgcgtcccg
     4621 tacgccggtg tcccggctgg cggggctggt gctgccctat ccgacccact cggaggccgg
     4681 caagcgggcg gccgggacgg cgtacgagcc gcggctgttc gcgccgatga cgcggcgtgt
     4741 ggtgggctgg ctgcagcggc tgtgacgcgg ggcgccgccg cataggcatc aggataagcg
     4801 tcgt
//
LOCUS       DASSZH010000023         2110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_375418, whole genome shotgun sequence.
ACCESSION   DASSZH010000023 DASSZH010000000
VERSION     DASSZH010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375418"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1524
                     /locus_tag="VFN42_00400"
     CDS             <1..1524
                     /locus_tag="VFN42_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995102.1"
                     /translation="QDSLLVDSSAWTRLAPEGFAAVVANPPWEKIKLSRHEFIKRNGG
                     TRQYGAAYAGHDLSGYETGRVAAAARSAALARLYPVLASGEPDLYVAFTALLLRLTRP
                     GGAGAVFVPGGMIRSQNTAGLRRLLLQCSRTLSFTVLENRAKFFAIDTRFKFVLCRFD
                     AAEPKGRKAGTIQLAHAYGTEAGVRLAAPVRMARKTLADLSPDLTIPEVRDRREWQLF
                     QKMQRAGLRWSDRASPWYPAFCREVDMTRETQHFRRSPGPDLLPVIEGRMVQPHRFGA
                     KAYQSGQGRSALWVAVPLGAARVVPQFWIAPAALPKSVAVRANMLRVGFCDITGQTNE
                     RSMMAALIPAGVVCGNKVPTILFPNDPSDDRLFLWAAIVNSLPFDWALRRVMTTTVNY
                     FLLLGLTLPRIEPDSLPGRRLVDIARRLALLDRAGPTGTEIGWRAADLRAEADAIVAM
                     SYGLSQDDLLLMFRDFPLLDRGQPALLDEPKSTVTRDCVLARFAALRRGASLNGPTG"
     gene            1853..>2110
                     /locus_tag="VFN42_00405"
     CDS             1853..>2110
                     /locus_tag="VFN42_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995103.1"
                     /translation="MLGLTAARGPRPAQHPTPHYGEPAGENAGTELDREIPALLTNAA
                     RQARLNGCRLEFAVPPQLAMRADRASFREAMSLFLAHAIRQA"
BASE COUNT          333 a          706 c          690 g          381 t
ORIGIN      
        1 caggacagcc tgctggtgga ttcgtccgca tggacgcgcc tggcgccgga gggtttcgct
       61 gccgtcgtgg ccaatccgcc ctgggagaag atcaagctgt cccgccacga gttcatcaag
      121 cggaatggcg gaacaaggca gtacggcgcg gcgtacgccg ggcatgatct gtccggctat
      181 gaaaccggcc gcgtggcggc ggcggcgcgg tcagcggcgc tggcgcggct gtatcccgtg
      241 ctggcgtcgg gcgaaccaga cctgtacgtg gccttcaccg cacttctgct gcgcctgacc
      301 cggcccggtg gcgccggtgc ggtgttcgtt ccgggcggca tgatccggtc gcagaacacc
      361 gctggactgc gccggctgct gctgcaatgc tcccgcaccc tgtcgttcac ggtcttggaa
      421 aaccgcgcga agttctttgc gatcgacacc aggttcaaat tcgtcctgtg ccggttcgat
      481 gcggcggagc cgaagggacg caaggcgggc acgatccaac tggcacatgc atacggcacc
      541 gaagccggag tcaggcttgc cgcgccggtg cggatggccc ggaaaacgct tgccgacctg
      601 tcacccgacc tcacgattcc cgaggtgcgg gatcggcgcg aatggcagtt gttccagaag
      661 atgcagcgcg ccggcctgcg atggtcggat cgcgcaagtc cgtggtaccc cgcgttctgc
      721 cgcgaagtcg acatgacgcg ggagacgcaa catttccgcc gatctcccgg accggatctg
      781 cttcccgtca tcgaaggccg catggtgcag ccgcatcggt tcggcgctaa agcctatcag
      841 agcggccagg ggcgcagcgc gctgtgggtc gctgtgccgc tgggcgccgc cagggtcgtg
      901 ccgcaattct ggatcgcccc ggccgccctg ccaaagagcg ttgccgtgcg cgcaaacatg
      961 ctccgcgtgg ggttctgcga tatcaccggc cagaccaacg aacgttcgat gatggccgcg
     1021 ctgatccccg cgggtgtggt gtgcggcaac aaggttccga cgatcttgtt tcccaacgac
     1081 ccatcggacg atcgcctgtt cctctgggca gccatcgtca acagcctgcc attcgattgg
     1141 gcgctacgcc gggtgatgac gacgaccgtc aattacttcc tgttgctggg gctaactctg
     1201 cccaggatcg aaccggacag cctgccgggg cgccgcctgg tggacatcgc ccggcgtctg
     1261 gcgctgctgg accgcgcggg gcccaccgga accgaaatcg gctggcgcgc ggcggacctg
     1321 cgcgccgaag ccgacgccat cgtggccatg tcctatgggc tgagccagga cgacctgttg
     1381 ctgatgttcc gcgactttcc gctgctggat cgcggccagc ccgcactcct ggacgagcca
     1441 aaatccacag tcacccggga ttgcgtgctg gcacgattcg ccgccctgcg tcgcggcgca
     1501 tcgctcaatg ggccgaccgg gtgaaggcgg ccaaactgcg cggcgccgtt gcctaccttc
     1561 cgggcgaagt ctcgagacgc ggcgagtccg tcagggctgc tgcctgagag aattcctgtc
     1621 atccgcatcg gcgagacggc ggcgggtcac caatccgtga tcccgggcga tcccagggtt
     1681 cctcagcctt tgtgaagcca cccttaatat ccggccacat caacacgtct tgttggttct
     1741 tctcggctca ccacttgtat agtcaggtgg ttgggtgtcc atccgatgtt gtgccgtgtc
     1801 attccgtgat ccaattcagt ggcttttcca ggctccggct tacgcatttg gcttgctggg
     1861 gctgaccgct gcgcgtggcc ccaggcctgc gcagcacccc acaccgcact acggcgagcc
     1921 ggccggcgag aatgccggaa ccgaactgga ccgcgaaatt cccgcactgc tgaccaacgc
     1981 cgcccggcag gcgcgactga acggctgccg gctggagttt gccgtgccgc cgcaactggc
     2041 catgcgcgcc gatcgcgcca gcttccggga ggccatgtcc ttgttcctgg cccatgccat
     2101 tcgccaggcg
//
LOCUS       DASSZH010000024         4206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1278883, whole genome shotgun sequence.
ACCESSION   DASSZH010000024 DASSZH010000000
VERSION     DASSZH010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278883"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VFN42_00410"
     CDS             complement(<1..731)
                     /locus_tag="VFN42_00410"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET8995104.1"
                     /translation="MTTRVELRHWRAFVTAAETEHFGAAAERLGITQPALSQLIRALE
                     AALGLRLFDRSGHRMRASAAARELLPQASAAVQQADIAERSGEAIGRRARRAIALGYV
                     GSAPFHPLFAGLIQGIEAVRPAIPLRLDQCSGTAQVRYLLDERIDVGIVRSPLPALQP
                     EIASLALARERMVLARPAAGRRDDGDSLSLAALAGAPFIVYVEQPHGGLRHLTLEACR
                     DAGFEPRIAQTVPQIATMLCLVGAGV"
     gene            complement(1018..1182)
                     /locus_tag="VFN42_00415"
     CDS             complement(1018..1182)
                     /locus_tag="VFN42_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995105.1"
                     /translation="MSDKPQSHSAPNPDRPAKKRVRLQREAAALRENLRKRKEQARER
                     AVERPPPKQD"
     gene            1385..2275
                     /locus_tag="VFN42_00420"
     CDS             1385..2275
                     /locus_tag="VFN42_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET8995106.1"
                     /translation="MSSLTYAAPTSVDEAVKLLANAAGNAKVLSGGTDLLVQLRSGRL
                     RPDLIVDTKKIPGVIGIRKQADGSFVIGAATPGAEVEANAELKKAWPGVVEAMDLIGS
                     TQIQGRCTLAGNLCNASPAADSVPALYAAAATAVVVGPDGKREVPVEQIPTAPGRTSL
                     AKGEFILEFKLPARPAHSGDAYLRFIPRTEMDIAVVGCGISVTLDAAGKCTAARVAMG
                     AVAPTVLLVPDAAKALIGHTLDDATLKALDAAAQAICKPINDKRGTIEYRTKVAGVLA
                     RRVAAIAFDRALGRAGAKTH"
     gene            2288..2773
                     /locus_tag="VFN42_00425"
     CDS             2288..2773
                     /locus_tag="VFN42_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET8995107.1"
                     /translation="MSKHHVTATINGEQVEFLCETQQTLLDSLRDDIGLTGSKEGCAS
                     GDCGACSVIMDGRLVCSCLVLSCECDGKTITTIEGIAQGDHLHPIQQKFLEHAALQCG
                     FCTPGLIVASKALLDENPNPTEEEARYWLAGNLCRCTGYDKVIRAVMDAAAEMRGEKT
                     A"
     gene            2770..>4206
                     /locus_tag="VFN42_00430"
     CDS             2770..>4206
                     /locus_tag="VFN42_00430"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET8995108.1"
                     /translation="MNYVDSNLKVVGTRPVRPDGVEKVTGRAQFGADMRVSGMIWGKV
                     LRSPHAHARILSIDTSKAEKLPGVKAVVTSKDFPHIANEEAFVGEGPMNFRDLSRNCL
                     AWDKALYEGHAVAAVAATSQSICDEALKLIDVKYEVLPYVIDVEDAMKPDAPVLHDDL
                     FTSGVKPAPDKPSNIAKKLTFQKGDIAAGFSQAEVVVEGYYTTKPVHQAYIEPHACVC
                     SYNADGQVNIWSSSQGHFMVRAYCAKLLGLDISNIRATPMEIGGGFGGKTLVYLEPVA
                     IALSKKAGRPVKMQMTREEVFRASGPTSGSTISVKFGAKKDGTIVAAQQELKYQAGAF
                     PGSPIGPGCMCGFAMYDIPNVDVVGYDVVSNRPKVAAYRAPGAPISSWAVECVMDDLA
                     RKLGKDPIALREQNGARDGTKTHYGVTHQNIGFLATLKAAKEHPHYKSALQPGQGRGV
                     ACGFWFNIGGESSAGCHIAEDGTVVATSG"
BASE COUNT          757 a         1409 c         1399 g          641 t
ORIGIN      
        1 acgccggcgc cgaccaggca cagcatggtg gcgatctgcg gcaccgtctg ggcaatacgg
       61 ggttcgaagc cggcatcgcg gcaggcttcc agggtcaggt ggcgcagccc gccatgcggc
      121 tgctccacat agacaatgaa tggggccccg gccaaggcgg caaggctcag cgaatcgcca
      181 tcgtcccggc ggccggcggc cgggcgggcg agcaccatcc gctcgcgggc cagcgccaga
      241 ctcgcaatct ccggttgcaa ggcgggcagc ggcgagcgga caatcccgac atcgatgcgc
      301 tcatcaagca ggtagcgcac ctgcgcggta cccgaacact ggtcgagccg cagcgggatg
      361 gcagggcgga ccgcctcgat gccctggatg aggccagcga acagcgggtg gaagggtgcg
      421 gaacccacat atcccagggc gatcgcccgg cgggctcggc ggccgatcgc ctcgcccgag
      481 cgttccgcga tatcggcctg ctgcaccgcc gcgctcgcct gcggcaacaa ctcgcgtgcc
      541 gccgcgctgg cacgcatgcg atgaccgctg cgatcgaaca gccgaaggcc gagggcggct
      601 tccagcgcgc ggatcaactg gctgagcgcg ggttgggtga tgcccagccg ttcagccgcc
      661 gcgccgaaat gctcggtttc cgccgccgtg acgaaggcgc gccagtggcg caattcgacc
      721 cgcgtggtca tgccgatcgc gatacgggat caaagtgtcg caagtgcctg aagctcgagc
      781 cccggcgaat ccagccggag cggacagcct cgaacgatcc gcgtcatgat ggcgccatcc
      841 tcgccgcggc cagttgcgcg cagatgagcc ctttccggcg ggaatgggcc gcacctccgg
      901 gggccatcgc gacgatttca ctggcaacca gctcgaagcg gctgccgccg ggctgctcca
      961 tcgcccaggc gatgaatgcg tccgatctca tccgcccgcg ggctggatcc gacatgttca
     1021 gtcctgcttc ggtggtgggc gttccacggc gcgctcccga gcctgctcct tgcgcttgcg
     1081 cagattctcg cgcaatgccg ccgcctcgcg ctgcagccga acccgcttct tcgccgggcg
     1141 atcgggattc ggcgcagagt gggactgtgg cttgtcggac atgcgacaaa gcatcgccaa
     1201 ccggagtccg gcgtcaaggg acccagcgtg gccacggcgc ctggtcgtaa cacaggtgtc
     1261 atgcgggctt ccgggcggcc cggctctttc ccctgcgcct ccgatcgcct aacgtcccag
     1321 tccgagccat ccgggcgcac cccgcccccg gagcggggaa acgcgaacaa aatcgggagc
     1381 tgtgatgtcg agtctcactt acgccgcgcc aaccagcgtg gacgaagccg ttaaattgtt
     1441 ggccaatgcc gccggtaacg ccaaggtact gtccggagga accgacctgc tggtgcagtt
     1501 gcgttcgggc cgtctgcggc cggacctgat tgtcgatacc aagaagattc ccggcgtcat
     1561 cggcatccgc aaacaggcgg acggcagttt cgtgatcggc gccgcgacgc cgggcgccga
     1621 ggtcgaagcc aacgccgagc tgaaaaaagc ctggccgggc gtcgtggagg caatggacct
     1681 gattggctct acgcagatcc agggccgctg caccctggcc ggcaatctgt gcaacgcctc
     1741 tccggccgcg gattcagtgc cggcgctgta cgccgccgct gccaccgcgg tggtggttgg
     1801 cccggatggc aagcgcgaag tcccggtgga acagatcccc accgcacccg gccgcaccag
     1861 tctggcgaag ggcgagttca tcctggaatt caagctccct gcccgcccgg cacattccgg
     1921 cgatgcctat ctgcgcttca tcccacgcac cgagatggat atcgcagtgg tcggttgcgg
     1981 catcagcgtc acgctggatg ctgccggcaa atgcaccgcc gcccgggttg cgatgggtgc
     2041 cgtcgccccc actgttctgc tggtgcccga cgccgccaag gcgctgatcg gacacaccct
     2101 ggacgacgcc acgctgaagg cgctggatgc cgccgcccag gcgatctgca agccgatcaa
     2161 cgacaagcgc ggcacgatcg aataccgcac gaaagtggcc ggcgtgctcg cccgccgcgt
     2221 cgccgccatc gcctttgacc gcgcgctcgg ccgcgccggc gccaagactc actgagagga
     2281 atttcgaatg agcaagcatc acgtcaccgc caccatcaac ggcgagcagg tcgagttcct
     2341 ctgcgaaacc cagcagactc tgcttgattc gctgcgcgac gacattgggt tgaccggcag
     2401 caaggaaggt tgcgcctccg gcgactgtgg cgcctgcagc gtcatcatgg acggcaggct
     2461 ggtctgctcc tgcctggtgc tgtcctgcga gtgcgacggt aagacgatca ccacgatcga
     2521 gggcatcgca cagggcgacc acctgcatcc aatccagcag aagttcctgg agcacgccgc
     2581 gctgcaatgc ggcttctgca ccccgggtct gatcgtcgcg tccaaggcgc tgctggacga
     2641 gaatccgaac ccgaccgagg aagaggcccg ctattggctg gccggcaacc tctgccgttg
     2701 caccggctat gacaaggtga tccgcgccgt catggatgcc gccgcggaaa tgcgcgggga
     2761 gaagaccgca tgaactacgt cgacagtaac ctgaaggtcg tcggcacgcg cccggtgcgc
     2821 cccgacggcg tggaaaaagt gaccggccgc gcacagttcg gcgccgacat gcgggtgtcg
     2881 gggatgatct ggggcaaggt gctgcgctcg ccgcacgctc atgcccgcat cctgtcgatc
     2941 gatacatcca aggcggagaa gctgcccggc gtgaaggcgg tggtgacgtc gaaagacttc
     3001 ccgcatatcg ccaatgagga agccttcgtc ggcgaagggc cgatgaactt ccgcgatctg
     3061 tcgcgcaact gcctggcctg ggacaaggcg ctgtacgaag gccacgcggt cgccgcggtc
     3121 gccgcaacca gccagtcgat ctgcgacgag gcgctgaagc tgatcgacgt gaagtacgag
     3181 gtgctgccgt acgtcatcga cgtcgaggac gcgatgaagc cggatgcgcc ggtgctgcac
     3241 gatgacctgt tcacctccgg ggtgaagccg gcaccggaca agccgtccaa catcgccaag
     3301 aagctgacct tccagaaggg cgacattgcc gccggcttca gccaggccga ggtggtggtg
     3361 gaaggctatt acaccaccaa gcccgtgcat caggcctata tcgagccgca tgcctgcgtt
     3421 tgcagctaca atgccgatgg ccaggtaaac atctggagca gcagccaggg ccacttcatg
     3481 gtgcgcgcct actgcgccaa gctgctgggc ctggacatct ccaacatccg cgcgacgccg
     3541 atggaaatcg gcggcgggtt cggcggtaag acgctggtgt acctggagcc ggtggcgatc
     3601 gcgctgtcga agaaggcggg ccgcccggtg aagatgcaga tgacccggga agaagtgttc
     3661 cgcgcgtccg gcccgacctc cggttccacc atcagcgtga agttcggcgc gaagaaggac
     3721 ggcaccatcg ttgccgcgca gcaggagctg aaataccagg cgggcgcctt ccccggctcg
     3781 ccgatcggac ccggctgcat gtgcggcttc gcgatgtacg acatcccgaa tgtcgatgtg
     3841 gtcggctacg acgtggtgtc caaccgcccc aaggtggcgg cgtaccgcgc gccgggggca
     3901 ccgatctcgt cctgggccgt tgagtgcgtg atggacgacc ttgcccgcaa actgggcaag
     3961 gatccgatcg cactgcgcga gcagaacggc gcacgtgacg gcaccaagac gcattacggc
     4021 gtgacccacc agaacatcgg cttcctcgcc acgctgaagg cggcgaagga gcacccgcat
     4081 tacaaatccg cgctgcagcc cggccaggga cgcggcgtcg cctgcgggtt ctggttcaac
     4141 atcggcggtg agtcctccgc cggctgccac atcgcggaag acggcacggt ggtcgccacc
     4201 tcgggc
//
LOCUS       DASSZH010000025         2378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1193655, whole genome shotgun sequence.
ACCESSION   DASSZH010000025 DASSZH010000000
VERSION     DASSZH010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193655"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VFN42_00435"
     CDS             <1..525
                     /locus_tag="VFN42_00435"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET8995109.1"
                     /translation="YGPEVLQHGSAEHARMRAVRTKMTIPPVMADIGAMLAFVDAQPA
                     AKPGPVGAHGYCMSGPYALAAAARYPDRIAAAASFYGTWLVSDAEESPHQNLSKPKGE
                     LYIACAEHDELAPLDMVDTLRRLFQRSGNSGQMELYLGVHHGFAFPQRWCYDKSAAER
                     HWERLIALYRRRLG"
     gene            794..>2378
                     /locus_tag="VFN42_00440"
     CDS             794..>2378
                     /locus_tag="VFN42_00440"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HET8995110.1"
                     /translation="MAVSSLLAGAPALAQTLPPAASQVPQGSPIPRILPPKPPAVAPG
                     AALPALPPPGVAVPNRPVRVSSVAVEGVTAYPAGSFAHDLQGLTGPAVPLPKINAARQ
                     AILQQYRSDGYVLTTVSASLDAAGKLRFIVTEGRIASVKLDGNIGPAGTQVLRFLHRL
                     TQQTPINAATLERYLLLAQDVPGVTLRAVLEPSADVPGALNLIAQVSRKPISGLLTFD
                     NRAFDQTGPIEGLGVVDFNSFTEFGEKTEVSWYHTFPNSQNFGQLSEEFYLGGSGLKL
                     HLYAGTGLAIPTGGGTGGLGLLDYHGRTNVFGGTLSYPVIRARQQTLNVYIAFDAIES
                     MIRTNTGSGGLSEASSDQLRVLRLGEDYARSDILFGAERAAVNAVSFRVSHGLHGLGA
                     DGNDADGSRLAPRVNEQTDFTKLNFEFSRTQTLFNPWQGGSVALMGLVAGQWSGDILP
                     PAEQFYLGGGRFTRGYYAGQVAGDKALAATAELQLNTDLNLTKWHIPRDLATQFYLFY
                     DWGETWQNAPDIQPVVIGSA"
BASE COUNT          400 a          797 c          796 g          385 t
ORIGIN      
        1 tacggcccgg aggtgctgca gcatggcagc gccgagcacg cgcgaatgcg ggcggtgcgc
       61 accaagatga cgattccgcc ggtgatggcg gatatcggcg cgatgctggc gttcgtggat
      121 gcgcagccgg cggcgaagcc cgggccggtc ggtgcacatg gctattgcat gagcggcccc
      181 tacgcactgg ccgccgccgc gcgctatccg gaccgcatcg ccgcggccgc gtcgttctac
      241 ggcacctggc tggtcagcga cgccgaggaa agcccgcacc agaacctgag caagccgaag
      301 ggcgagctgt acatcgcctg cgccgaacac gacgagctgg cgccgctgga catggtggac
      361 acgttgcgcc ggctgttcca gcgctccggg aattccgggc agatggaact gtacctgggc
      421 gtgcatcacg gcttcgcctt tccgcagcgc tggtgctatg acaaatcggc tgcggaacga
      481 cactgggagc gcctgatcgc gctgtatcgg cgccgcctgg ggtaaccggt ggcgccgacg
      541 ccgccggccg cctgtgccac cggaagacaa ataccggacg ttgcagccgg accgagtcgg
      601 cgcacccata gggtgcttcc acaagaaatt cacggacgtt tcttgacggc ggggcacagt
      661 tacgcgatcg ttgcataggc tcagatcgaa gcaagagcga aacagaggca gcaccgtggc
      721 agcatggagg ggaggccggc ggcgaggcga tacagccttc gccgcgatca gcgggtacac
      781 cgcgatcgga ttgatggcgg tatcgagctt gctggcggga gcgccggctt tggcgcagac
      841 cctgccaccc gcggccagtc aggttccgca gggttcgcct atcccgcgca tcctgccgcc
      901 caaaccgccg gcggtcgcgc ccggggcggc attgccggca ttgccgccac ctggtgtcgc
      961 ggtaccaaac cgtcccgtgc gggtctccag cgtcgccgtc gagggcgtga ccgcctaccc
     1021 cgccggcagc ttcgcgcatg atctgcaagg cctgaccgga cctgcggtgc cgctgccgaa
     1081 gatcaacgcc gcgcggcagg cgatcctgca gcagtaccga tccgacggct atgtgctgac
     1141 cacggtgtcc gccagcctcg acgcggccgg caagctgcgg ttcatcgtca ccgaaggccg
     1201 aatcgccagc gtcaagctgg acggcaatat cgggccggcc ggcacgcagg tgctgcgctt
     1261 cctgcatcgg ctgacgcagc agacgccgat caacgcggcc acgctggagc gctacctgct
     1321 gctggcgcag gacgtgccgg gggttaccct gcgcgcggtg ctggagccgt ctgccgacgt
     1381 gccgggcgca ctgaatctga tcgcccaggt ctcgcgcaag ccgatttccg gcctgctgac
     1441 cttcgacaac cgcgccttcg accagaccgg gccgatcgag gggcttggcg tcgtcgactt
     1501 caacagcttc accgagttcg gcgagaagac ggaagtctcc tggtatcaca ccttccccaa
     1561 cagccagaat ttcggccagc tctctgagga gttttacctt ggcggttccg gcctgaagct
     1621 gcacctgtat gccggcaccg gccttgccat tcccaccggc ggcggcaccg gtggcctcgg
     1681 gctgctggat taccatggcc gcaccaatgt gttcggcggc accctgagct atccggtgat
     1741 ccgtgcccgc cagcagacgc tgaacgtcta tatcgcgttc gacgccatcg aatcgatgat
     1801 ccgcaccaac accggcagcg gcggcttgtc ggaggccagc tccgaccagc tgcgggtgtt
     1861 gcggctgggc gaggattatg cccgctccga catcctgttc ggcgccgagc gtgccgcggt
     1921 gaacgcggta tcgtttcgcg tctcacacgg cctgcacggg ctgggcgccg atgggaacga
     1981 cgccgacggc tcacggctgg cgccgcgcgt caacgagcag accgacttca ccaagctgaa
     2041 cttcgaattc agccggacgc agacgctgtt caacccatgg cagggcggca gcgtcgcgct
     2101 gatggggctg gtggccggtc agtggtccgg tgacatattg ccgcctgcgg aacagttcta
     2161 cctcggcggc ggacgcttca cccgcggcta ctatgccggc caggtcgccg gcgacaaggc
     2221 actggctgcc accgcggaat tgcagctgaa caccgacctg aatctgacga agtggcacat
     2281 tccgcgcgat ctcgcgacac agttctacct gttctacgac tggggcgaga cttggcagaa
     2341 cgcgcccgat atccagccgg tggtgatcgg ttcggccg
//
LOCUS       DASSZH010000026         2555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1296005, whole genome shotgun sequence.
ACCESSION   DASSZH010000026 DASSZH010000000
VERSION     DASSZH010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296005"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="VFN42_00445"
     CDS             complement(<1..140)
                     /locus_tag="VFN42_00445"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET8995111.1"
                     /translation="MTGSSPPLIQDIAEELRGIGFTEYEAQAYIALLQENPATAYQVG
                     KQT"
     gene            complement(148..906)
                     /locus_tag="VFN42_00450"
     CDS             complement(148..906)
                     /locus_tag="VFN42_00450"
                     /EC_number="4.4.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HET8995112.1"
                     /translation="MSFALPSRSAKPRSRGITAMIDFGPDTFGWTGRAGVADLIACAG
                     AYIDYAKIYALNSLLMPEAEVARIVRMYAEAGIVPYSGGILFEYAYQQDQLDAMLALL
                     DRLGLRTMELSENYVSLAEDERLALIARFHARGFQVIYEFGRKNPEAPLTIAELHRIV
                     ASVAELGVHHVIVEQSEIDVLADGAMAELTRQPWFARILVETDPYRFPQQHADLLTEY
                     GPEINLANVTPGQVLRLEGLRRGIGRAVNYRLLG"
     gene            complement(910..2151)
                     /locus_tag="VFN42_00455"
     CDS             complement(910..2151)
                     /locus_tag="VFN42_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrpF domain-containing protein"
                     /protein_id="HET8995113.1"
                     /translation="MASPLSAPLEARARAYPRQHAVPCLFMRGGSSRGGFFLAADLPA
                     DERERAAWLLAAYGSPDARQIDGIGGSDPLTSKAAIVGPASRDDADVDYSFAQVSLAA
                     AQIGIGGTCGNMLAGVGPFAILRGLVEAREPETVVRIHATNTGQVITARVPVLDGMPA
                     IEGGCEIPGVPGSGARIGLDFGDCAGAVSGRLLPTGSPRDEIVLAGRRTRVSLVDAAT
                     PFVFVCAQDIGARGTESAAEIAGNEELQERLELVRGWAATVLAMAPSAEAAREVSPNV
                     PRVIMVAPPQDYLTPEKTRIEAAAADICVRQMSMQRPHKALAVTGSICTAVAAAVAGT
                     TVAEMAAQWRGGPLRLGHPSGVLQVRAVVHRDDAGYRIQSAEIERTARPIMDGVLYVP
                     MAKVAALANVVEISGAKPSGD"
     gene            complement(2123..>2555)
                     /locus_tag="VFN42_00460"
     CDS             complement(2123..>2555)
                     /locus_tag="VFN42_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HET8995114.1"
                     /translation="NLNVAMGSTQFGVSLALALGGNGLRDYWAGFNMPEVHEAAANRV
                     RLVAEPSFGLGGRQAILEVRLKDGRLLQQRSEEPRGEPTNPLTDEEMDRKFLGMAGMA
                     IDDAQAQELSTRLMALQNETRAAVIPGMTVAANGKPALRAA"
BASE COUNT          410 a          929 c          816 g          400 t
ORIGIN      
        1 gtctgcttgc ccacctgata ggcggtcgcg gggttttcct gcagcagcgc gatataggcc
       61 tgtgcctcgt actcggtgaa accgatcccg cgcagctcct cagcgatatc ctggatcagg
      121 ggtggcgagg aaccggtcat cgatgcatca gcccagcagc cggtaattca ccgcacggcc
      181 gatcccgcgc cgcaggcctt ccagccgcaa cacctgcccc ggcgtcacat tggccaggtt
      241 gatctccggg ccgtactccg tcagcaggtc cgcgtgctgc tgcgggaaac ggtacggatc
      301 ggtctccacc agaatccgcg cgaaccacgg ctgccgcgtc agctcggcca tcgcgccgtc
      361 cgccaggaca tcgatctcgc tctgctcgac gatcacatgg tgaacgccca gctccgccac
      421 cgatgcgacg atccgatgta gttcagcgat ggtcagcggt gcctcgggat tcttccgccc
      481 gaactcgtag atcacctgga agccgcgtgc gtggaaacgc gcaatcagcg ccaggcgctc
      541 atcctccgca aggctgacgt agttttccga aagctccatc gtgcgcaggc caagccggtc
      601 caacagggcg agcatcgcgt ccagctgatc ctgttggtag gcgtattcga acaggatacc
      661 gccgctatag ggaacgatgc cggcctcggc atacatgcgg acgatgcgcg ccacttccgc
      721 ttccggcatc agcagcgaat tcagcgcata gatcttggca tagtcgatat aggcgccggc
      781 gcaggcgatc aggtcggcaa ccccggcgcg tccggtccag ccgaacgtgt cgggaccgaa
      841 gtcgatcatc gcggtgatgc cgcgcgatct gggcttcgcg gatcgtgacg gcagggcgaa
      901 ggacatcgct cagtccccgg acggcttggc gccggaaatc tcgacgacgt tggccagcgc
      961 cgcgaccttc gccatcggca cgtacagcac gccgtccatg atcgggcggg cggtgcgttc
     1021 gatttcggcc gattggatgc gatagccggc atcgtcgcga tgcaccacgg cacgcacctg
     1081 cagcacgccg ctgggatggc ccaggcgcag cgggccgcca cgccactgcg cggccatctc
     1141 cgccaccgtg gtgccggcaa ccgcagcggc caccgcggtg cagatggagc cggtgacggc
     1201 cagcgccttg tgcggacgct gcatcgacat ctgccgcacg cagatatcgg ccgcggccgc
     1261 ctcgatccgc gtcttttccg gcgtcaggta atcctgcggc ggcgcgacca tgatgacgcg
     1321 cgggacattg ggactgacct cgcgcgccgc ctcggccgac ggcgccatgg cgagcacggt
     1381 cgcggcccaa ccgcgcacca gttccagacg ctcctgcaat tcctcgtttc ccgcgatctc
     1441 cgcggcgctt tcggtgccgc gtgcgccgat atcctgcgcg cacacgaaga caaacggcgt
     1501 cgccgcatcg accagcgaaa cgcgtgtcct ccgcccggcc aggacgatct cgtcacgcgg
     1561 cgatccggtc ggcagcagcc gtccggaaac cgcgccggcg caatcgccga aatcgaggcc
     1621 gatgcgcgcg ccgctgcccg gcacccccgg gatttcgcag ccgccctcga tcgccggcat
     1681 gccgtccagc accggcacgc gggcggtgat cacctggccg gtattggtgg cgtggatgcg
     1741 caccaccgtt tccggctcgc gcgcctccac caggccgcgc aggatggcga acggccctac
     1801 ccccgccagc atgttgccgc aggtgccgcc gatgccgatc tgcgccgcgg ccagcgacac
     1861 ctgggcgaag ctgtaatcga cgtcggcatc gtcgcggctt gccggcccga cgatcgccgc
     1921 cttgctggtc agcggatcgc tgccgccgat gccatcgatc tgccgggcat ccggcgaacc
     1981 ataggcggcc agcagccagg cggcgcgctc ccgctcatcc gccggcaggt cggcggccag
     2041 gaagaagccg ccgcggctgg agccgccccg catgaacagg catggcaccg catgctggcg
     2101 cggataggcg cgcgcccgcg cctcaagcgg cgcggagagc gggcttgcca ttggctgcca
     2161 ccgtcatgcc agggataacc gccgcccggg tctcgttctg caacgccatc aggcgcgtgc
     2221 tgagttcctg tgcctgcgca tcgtcgatcg ccatgcccgc catgccgagg aacttgcgat
     2281 ccatctcctc gtcggtcagc ggattggtcg gttcgccacg cggctcttcc gaacgctgct
     2341 gcagcagacg cccgtccttg agccggacct ccagaatcgc ctgccgcccg cccaggccga
     2401 aggacggttc cgccaccagc cgcacgcggt tggctgccgc ttcgtgcacc tccggcatgt
     2461 tgaacccggc ccagtagtcg cgcagcccgt tgccgcccag cgccagggcc agcgacacgc
     2521 cgaattgcgt gctgcccatg gcaacattca gattc
//
LOCUS       DASSZH010000027         6631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_614265, whole genome shotgun sequence.
ACCESSION   DASSZH010000027 DASSZH010000000
VERSION     DASSZH010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6631
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614265"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..597)
                     /locus_tag="VFN42_00465"
     CDS             complement(121..597)
                     /locus_tag="VFN42_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995115.1"
                     /translation="MFNPKRPGSHDGGLISGTLESALTASAVVLMLASVIILVALIST
                     AFVAPRVGDVVRFRPGSTVAEDLTFPAHRAGTAAQPGASCVLDPATMVQGGGSLVVES
                     RFTKLHEYQVHWAGQRTASGAQDCGRSVDLTLRQSDLETLVNAMGGRGLVGRGDVF"
     gene            993..1895
                     /locus_tag="VFN42_00470"
     CDS             993..1895
                     /locus_tag="VFN42_00470"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET8995116.1"
                     /translation="MMRASVQDAPLRGIALSLASTVLFASADTISKFLARSLPVIEIA
                     WIRYVVFVGMAAWLACRPGSRNLWPRNVPLQVARGLCLVGSAVLFVFGLRQMAMAEAA
                     TISFISPLLITVLSIPLLGERVGLRRWAAVVAGLAGVVIVMRPGLAGFQPAALYGVAS
                     SACWALALVITRRMAGSERPPTILLWSAGSGLLVLTLLLPWQAVLPTPAQLGLALVLG
                     VLASSGQWLVVLAFRHAPASLLAPFSYTQLLWSSFYGWLVFQALPDRWTLAGAAVIVA
                     SGLYTLHREGLNGQAIRRPAVQRP"
     gene            complement(1883..2077)
                     /locus_tag="VFN42_00475"
     CDS             complement(1883..2077)
                     /locus_tag="VFN42_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995117.1"
                     /translation="MRILLAVTLLALLSACAAQQSTGKDAMLPGIGPHAARVQADVQN
                     ILRGMDQARERDERVARYGR"
     gene            2237..3928
                     /locus_tag="VFN42_00480"
     CDS             2237..3928
                     /locus_tag="VFN42_00480"
                     /inference="COORDINATES: protein motif:HMM:NF011291.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HET8995118.1"
                     /translation="MGTTEAATEPGRDFVRDIIQSDLDAGRVREVVTRFPPEPNGYLH
                     IGHAKSICLNFGVAQEYGGRCHLRFDDTNPAKEEQEYIDAIQRDVRWLGFDWGSHLYH
                     ASDYFEQLYAWAEHLIRAGKAYVDDSTPTQMREQRGTLTEPGQNSRYRTRPVEENLDL
                     FRRMRAGEFPNGARVLRAKIDMASGNMNLRDPVLYRILHASHPRTGTKWCIYPTYDFA
                     HGQSDAIEGITHSICTLEFEDHRPLYDWFIDNLPVPSRPHQYEFARLNLAYTVLSKRV
                     LTKLVHEGHVTGWDDPRMPTIAGLRRRGVPPEALREFVRRVGVARAYSIVDYALYEHS
                     VRDVLNKTALRRMAVLRPLKLVIENYPEGQTETLEAANNPEDPGAGARPVTFGRELYV
                     ERDDFMENPPKKFYRLSPGREVRLRYGYFVTCREVVKDAAGTVVELRCTYDPATRGGN
                     APDGRKVQATLHWVAAADAVPAEVRLYSHLFARPDPGADGDVFADLNPDSLEVLTGCM
                     LEPALAQAAVGEAVQFERQGYFCLDRDTGPGALVFNRTTGLRDTWAKLQASGGRT"
     gene            4387..5634
                     /locus_tag="VFN42_00485"
     CDS             4387..5634
                     /locus_tag="VFN42_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HET8995119.1"
                     /translation="MPVQTNQRIDGKRLWDLLMSMAEIGATPKGGVRRLTLTEVDKRG
                     RDRFRAECEAAGLSVRVDAIGNMFARREGRDPKRLPVLFGSHLDSQPSGGKFDGALGV
                     LAGLEVIRSLNDLGIVTEAPLELVNWTDEEGSRFGHSLMGSGVWAGVYTLDKAYALTD
                     VEGVSVRSALDGIGYHGDNPAAPFPADAYFELHIEQGPILEREEKQIGIVTGAQAQVW
                     YDAVAIGQDSHAGTTPPSTRKDALVCAARIIDLVDRMMRARGEDGRGTVGQLLVSPNS
                     RNVVPGEVRFSVEFRHPSDDEVDRLDAQFPREAGFIARDCGVKLELTKLFKIPAQPFD
                     PACVELVRQGAAKLGYSAREIVSGAGHDAVYVARHVPTAMIFTPCKDGLSHNEAESIL
                     PEEAEAGCQVLFEAVLARANRTM"
     gene            complement(5675..>6631)
                     /locus_tag="VFN42_00490"
     CDS             complement(5675..>6631)
                     /locus_tag="VFN42_00490"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET8995120.1"
                     /translation="EIAARIVLDMQQHGGLLSMDDLAACIPEETPPLWGTYRGYAIAT
                     NNPPGGGIQLLEMLNILEHFDLRALGHNSPEYIRVVAEAMKIATADKDAKVGDPRFVR
                     VPVEELTSKHYAARMAERIRAGEKTLVPRFNSGGAESKHTTQISVMDEHGNAVTMTHT
                     LGQPSGVVTEGLGFMYSGAMAVFDPRPGHAGSLEPGKSRFSALSPTIVFDGGKPLLVL
                     GAPGATYITMGNLQAMLNVLDFGMDAQQAVQAPRFAAVSDTIELSNRILRATERALHG
                     FGYRTQRKAGSYEFARVHAIRMVDGKLDGGADPAAGGLVVAV"
BASE COUNT         1025 a         2176 c         2339 g         1091 t
ORIGIN      
        1 caaggccaag ccgatggcga aggcgaggtg gcgcagcaag gcatgcaagg tcatcggcgg
       61 gggccggtct ccacggaggg cagagcagta tcacgttccg ccgcggacgg aagccgccgc
      121 ctagaacacg tcaccgcgcc cgaccaggcc gcgcccgccc atggcgttga ccagggtttc
      181 cagatccgac tggcgcagcg tcagatcgac cgagcggccg caatcctgcg cgcctgaagc
      241 ggtccgctgg cccgcccaat gcacttggta ttcgtgcagc ttcgtgaacc ggctttccac
      301 caccaggctg ccgccgccct gcaccatcgt ggccggatcg agcacgcagg acgccccagg
      361 ctgcgcggcg gttccggccc gatgcgcggg gaaggtcagg tcctcggcca cggtcgagcc
      421 gggccggaac cgcaccacgt cgcccaccct gggcgcgacg aaggccgtgg agatcaacgc
      481 caccaggatg atgacgctgg ccagcatcag caccaccgcg ctggcggtca gcgcgctttc
      541 gagggtgccg gagatcagcc cgccatcatg cgagccggga cgtttgggat tgaacatcac
      601 agcgcttccg aaatctgcgc cgctgcgcca ccgggcagcc ggcatgggtc ctgggttgga
      661 tccctcctgg acggaaccgg gcaggcgtcg gacgcaccgc cgcaaacagc tgcctgggac
      721 gcgggaagcc ggatcggaac agggggcatg acgaaacctc ggctgtcttg ctcagattct
      781 gccccttgga cgcgcgccgg ccggcgcgat cggcgtttta tcgttcgcca agaatatcgc
      841 tattgtcttc gcacatgcag catcacgaac ccgtgaaatc cttctggtcg gggttgcgcc
      901 ggatgcatgg ccaaccgggc ctggccacca tcttttcgcc ctttccggcc cggaagctgc
      961 attgatccgc cgctcggaag aggaccggcc agatgatgcg ggcttcggtc caggacgctc
     1021 cgctccgcgg catcgccctg tcgctggcct cgacggtcct gttcgccagt gccgacacga
     1081 tctcgaaatt cctcgcccgc tcgctgccgg tgatcgagat cgcctggatc cgctacgtgg
     1141 tgttcgtcgg catggcggcc tggctggcct gccgcccggg cagccgcaat ctttggccgc
     1201 ggaacgtgcc attgcaggtg gcgcgcgggc tgtgcctggt gggatcggcg gtgctgttcg
     1261 tcttcggcct ccgccagatg gcgatggccg aggccgccac gatcagcttc atctcccccc
     1321 tgctgatcac cgtcctgtcg atcccgctgc tgggtgagcg ggtcggcctc cgccgttggg
     1381 ccgcggtggt cgccggactg gcgggcgttg tcatcgtaat gcggccgggg ctggctgggt
     1441 tccagcccgc ggcgctgtac ggggtcgcat cttcggcatg ttgggcattg gcgctggtca
     1501 tcacccggag gatggcaggc agcgagcggc cgccgaccat cctgctgtgg tcagcgggaa
     1561 gcggcttgct ggtgctgacc ctgctgctgc cctggcaggc cgtcctgccg accccggccc
     1621 agctcggctt ggccctggtc ctgggggtgc tggcatccag cgggcaatgg ctggtggtgc
     1681 tggcgttccg ccatgccccc gcctccttgc tggcgccctt ctcctacacc cagttgctgt
     1741 ggtcgagctt ctacggctgg ctggtgttcc aggcgctgcc ggaccgttgg acgctggccg
     1801 gcgccgcggt catcgtggcc agcggcctct atacgctgca tcgcgaaggc ctgaacgggc
     1861 aggcgatccg ccgcccggcg gttcagcggc cgtagcgcgc cacgcgctcg tcccgctccc
     1921 gggcctggtc catgccgcgc aggatattct gcacgtcggc ctgcacgcga gccgcatgcg
     1981 ggccgatgcc cggcagcatg gcatccttcc cggtggattg ctgcgcggca caggcgctca
     2041 gaagggccaa taatgtcact gccaagagaa ttcgcatcga actcgccgcc tcgtgatcct
     2101 ggctcgcttt tacgggagcc tcgcggcgcc ggccagcatg atgttgcgtt tggccggcag
     2161 gcggaccatc acaactcccg cgatgcgccg gccggacggc tggcgcgcgg cagtttttcg
     2221 cctggagccg agggccatgg gtacgacgga agcagcaacc gaaccgggcc gcgatttcgt
     2281 ccgcgacatc atccagtccg acctggacgc cggacgggtg cgggaggtgg tgacccgttt
     2341 cccaccggaa ccgaacggtt acctgcatat cggccacgcc aagtcgatct gcctgaactt
     2401 cggcgtggcg caggaatatg gcggacgctg ccacctgcgc ttcgacgaca ccaacccggc
     2461 gaaggaggag caggagtaca tcgatgcgat ccagcgcgac gtacgctggc tgggcttcga
     2521 ctggggcagc cacctgtacc acgcctcgga ttacttcgag cagctctacg catgggctga
     2581 gcacctgatc cgcgcgggaa aggcctatgt cgatgattcg acgccgacgc agatgcggga
     2641 acagcgcggc acgctgaccg agccggggca gaacagccgg taccgcacgc gcccggtgga
     2701 ggagaacctg gacctgttcc gccgcatgcg tgccggtgag ttccccaacg gcgcgcgtgt
     2761 gctgcgggcg aagatcgaca tggcgtcggg caacatgaac ctgcgcgacc cggtgctgta
     2821 ccgcatcctg cacgcgtccc acccgcgcac cggcacgaaa tggtgcatct atccgaccta
     2881 tgatttcgcg cacggccagt cggacgcgat cgagggcatc actcactcga tctgcacgct
     2941 ggaattcgag gaccaccggc cgctgtatga ctggttcatc gacaatctgc cggtgccctc
     3001 gcgcccccac cagtacgaat ttgctcggct gaacctggcg tacaccgtgc tgtcgaagcg
     3061 ggtgctgacg aagctggtgc atgagggcca tgtcaccggc tgggacgatc cgcgcatgcc
     3121 caccattgcc gggctgcgcc gccgcggcgt gccgccggaa gcgctgcgcg agttcgtgcg
     3181 ccgggtcggc gtggcgcggg cctacagcat cgtggactac gcgctgtacg agcactcggt
     3241 gcgcgacgtg ctgaacaaga ccgcgctgcg ccggatggcg gtactgcggc cgctgaagct
     3301 ggtgatcgag aactacccgg aagggcagac ggagaccctg gaggcggcga acaacccgga
     3361 agatcccggc gccggcgcgc gcccggtcac cttcgggcgg gagctgtatg tcgagcggga
     3421 cgatttcatg gagaacccgc cgaagaagtt ttatcggctg tcgcccgggc gggaggtgcg
     3481 cctgcgctac ggctatttcg tcacctgccg cgaagtggtg aaggacgccg ccggcacggt
     3541 ggtggagctg cgctgcacct acgatccggc gacgcgcggc ggcaatgcgc cggatggccg
     3601 gaaggtgcag gcgacgctgc actgggtggc cgcggcggac gccgtgccgg ccgaggtgcg
     3661 gctgtacagc cacctgtttg cccgcccgga ccccggcgcc gatggcgacg tgttcgccga
     3721 cctgaatccg gactcgctgg aggtgctgac cggttgcatg ctcgaacccg ccctggcgca
     3781 ggccgcggtg ggcgaggcgg tgcagttcga gcggcagggc tatttctgcc tggaccgcga
     3841 cactggtccg ggcgcgctgg tgttcaaccg caccaccggg ctgcgcgaca cctgggcaaa
     3901 gctgcaagcc tcgggcgggc gcacgtagcg cgccagggtc ggccggatcg cgcgcgtttg
     3961 gggtgatagc gcggcgggac ggtgcggatt gcgtcggctg tcatgccacc ctccgtgtca
     4021 tgcccgtgct tggcacgggc acccacgtcc tggtcggacg cagcagccgc aagccgtggg
     4081 tggccgcgcc aagcgcggcc atgacacctc ggtaagtggt gtcactcggc cgcggcggta
     4141 gccgtcgtcg tcgtcatgcg acggtttgat caggctgagg cgaaatccac ggctgttggc
     4201 ggtcatggtt tccgtcccca acaaccagca tttttaatac gtgttatgcc gggcgcgcca
     4261 agatttttgc tgtttcgctg cgccgcagag aagtttgccg ggaatgccag gggcgccggt
     4321 gtcaagcaat cgccgcggcg atatactgtc gcaggtttga tcgtctcacc gaccggagga
     4381 ggttccatgc ccgtgcagac caaccagcgc attgacggca agcggctctg ggacctgctg
     4441 atgtcgatgg cggagatcgg cgccacgccg aagggcggcg tgcggcggct gacgctgacg
     4501 gaggtggaca agcgcgggcg cgaccggttc cgcgccgaat gtgaagccgc cggactgagc
     4561 gtgcgggtgg acgcgatcgg caacatgttc gcccgccgcg agggccgcga tcccaagcgc
     4621 ctgccggtgc tgttcggcag ccatctggac agccagccga gcggcggcaa gttcgacggc
     4681 gcgctgggcg tgctggcggg gctggaggtg atccgcagcc tgaacgacct gggcatcgtc
     4741 accgaggcgc cgctggagct ggtgaactgg acggatgagg aaggcagccg gttcggccat
     4801 tcgctgatgg gctccggcgt gtgggccggc gtgtacacgc tggacaaggc ctatgcactg
     4861 accgacgtgg aaggtgtgag cgtgcgcagc gcgctggatg gcattggcta tcacggtgac
     4921 aatcccgccg cgccgttccc cgccgatgcg tatttcgagc tgcatatcga gcagggaccg
     4981 atcctggagc gcgaggaaaa gcagatcggc attgtcaccg gcgcgcaggc gcaggtgtgg
     5041 tacgacgcgg tggcgatcgg gcaggacagc cacgccggca ccacgccgcc ttcgacgcgc
     5101 aaggacgcgc tggtctgcgc tgcgcggatc atcgacctgg tggaccgcat gatgcgcgcg
     5161 cgcggcgagg acggccgcgg cacggtgggg cagcttctgg tgtcgccgaa cagccgcaac
     5221 gtggtgccgg gcgaggtgcg gttctcggtg gagttccgcc atccgtcgga tgacgaagta
     5281 gaccggctgg acgcgcagtt cccgcgcgag gcggggttca tcgcgcgcga ttgcggggtg
     5341 aagctggagc tgacgaagct gttcaagatt ccggcgcagc cgttcgatcc ggcctgtgtc
     5401 gagctggtgc ggcagggcgc ggcgaagctt ggctactccg cgcgggagat cgtgtcgggc
     5461 gccgggcatg acgcggtgta cgtcgctcgg cacgtgccga ccgcgatgat cttcacgccg
     5521 tgcaaggacg ggctgagcca caacgaggcg gagagcatcc tgccggaaga ggccgaggcc
     5581 ggctgccagg tgctgttcga agcggtgctg gcgcgggcga accggacgat gtgacgattc
     5641 ctcccccacc ccttgcggga gggggaggag tccgtcacac cgccaccacc aaacctcccg
     5701 cagccgggtc ggcgccgccg tcgagcttgc cgtcaaccat gcggatcgcg tgcactcggg
     5761 cgaactcgta actgcccgcc ttgcgctgcg tgcggtagcc gaagccgtgc agcgcgcgtt
     5821 cggtggcgcg caggatgcgg ttggacagct cgatcgtgtc ggacacggcg gcgaagcgcg
     5881 gggcctgcac cgcctgctgc gcgtccatgc cgaaatccag cacgttcagc atcgcctgca
     5941 ggttgcccat ggtgatgtag gtcgcgcccg gcgccccgag caccagcagg ggcttgccgc
     6001 cgtcgaacac gatggttggc gacaacgcgg agaagcgcga cttgcccggc tccagcgaac
     6061 ccgcatgacc ggggcgcgga tcgaacaccg ccatcgcacc gctgtacatg aagcccagcc
     6121 cctcggtgac cacgcccgat ggctggccca gcgtgtgggt catcgtcacc gcattgccgt
     6181 gttcgtccat taccgagatc tgcgtggtgt gcttgctctc ggcgccgccg ctgttgaaac
     6241 gggggacgag cgtcttctcg ccggcgcgga tgcgctccgc catgcgcgcc gcatagtgtt
     6301 tggaggtcag ttcctccacc ggcacgcgca cgaagcgtgg atcgccgacc ttggcgtctt
     6361 tgtcggcggt ggcgatcttc atcgcctccg ccaccacgcg gatatattcc ggcgaattgt
     6421 gccccagcgc gcgcaggtcg aaatgctcca ggatgttgag catctccagc agctggatgc
     6481 cgccgccggg cggattgttg gtggcgatcg cgtaaccgcg ataggtgccc cagagcggcg
     6541 gagtctcctc cggaatgcaa gcggcgagat cgtccatcga gagcagcccg ccgtgctgct
     6601 gcatgtccag cacgatgcgc gcggcgatct c
//
LOCUS       DASSZH010000028         2559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_716493, whole genome shotgun sequence.
ACCESSION   DASSZH010000028 DASSZH010000000
VERSION     DASSZH010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716493"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..2336
                     /locus_tag="VFN42_00495"
     CDS             228..2336
                     /locus_tag="VFN42_00495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8995121.1"
                     /translation="MKPDLSLILNLHNETRFLARTLRSSEEAVRYAAAAGITTELVFV
                     LDRPAADTQAWVARYQADCFAAIRSVVVDNGSLGPSRVDGVAIAQGEYVTLADGDDLI
                     SFNALAAAMQMARTAGPEAIVLPEYVLEFGDRHYVTAYYGDPVVGAIQTLFSHPFNSR
                     ILIRRDRFAAIGCRDIPLGTGYAYEDWHFNSEALARGMRFVMAPDTVLFYRRRPSSLS
                     AGSRSISTGQIPPTTLAEPETVRTRLAAEYAAWTQGCAAQRHRRMLDQVEPRTRAFLA
                     GPLFHELAFAANRIDAAVEANRYFGTHVWTNLREPTHIGLAYCRLCEIVGNRRFTDVF
                     LLPFFVRGGGEKYLLNVMHGLMQTRPGTSILVLTGEPRQQHDWLDRLPAGSAFVDLWQ
                     VGPDCTRADIELLTLKAIQALAPSARIHLKHCTYAFQFWNRFGRVLADSNRGILYRFL
                     DTCYQDHGRRFLGGFEFDFISNNLDTFHAIVTDHRKIIDADRQRFDHRHEVWDWLPTE
                     CQPTRSPEQLEAAPSGWRRRLLWASRLDDQKRPHLLPLLAAALRRRGIQISIDVYGAA
                     VLQNYDVAALSCEGLCYRGAFEAFEALDCEAYDGFIYTAEFDGLPNVVLEALGAGLPV
                     IAPDIGGIAEVVQPGQTGWLLPSDGSEAALVDGFCDAIEALYSDPQRQRRMAVAAVRL
                     IMSRHGRTAFLRRIERIFGD"
     gene            2339..>2559
                     /locus_tag="VFN42_00500"
     CDS             2339..>2559
                     /locus_tag="VFN42_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET8995122.1"
                     /translation="MATERLHMSGEQPYHGFEAAAHVARYALLRPACAQRRVLDIACG
                     EGYGSALLAGWGARSVVGVDISAAAIASA"
BASE COUNT          371 a          887 c          843 g          458 t
ORIGIN      
        1 gccctggcgt ccatggtccg gcggtccctg gtcccggagt ccctggccct ggagtccgtg
       61 gcccggcagt ccatggtctg gcggtccctg gtccggcagt ggaaacctgc gtcggatttg
      121 tgccatattg cactggcggc aaggagagcc gcgcgcctga ggttcgccgc ggcacggacc
      181 tgcggccgtc gcatcgccgc tccccggacg cgtgcgaccg tgctgccgtg aagcccgacc
      241 tgagcctgat cctcaacctg cacaacgaaa cccgcttcct ggcgcggacc ctgcgttctt
      301 ccgaggaagc ggtccgctac gccgcggccg ccggcatcac caccgaactg gtgttcgtgc
      361 tcgaccggcc cgccgcggat acccaggcct gggtggcgcg gtaccaggcc gattgtttcg
      421 cggcgatccg cagtgttgtc gtggacaacg gctcactcgg tccctcccgc gtggacggcg
      481 tggccatcgc gcagggcgag tatgtcacgc tggccgacgg cgatgacctg atctccttca
      541 atgctctggc cgctgcgatg cagatggcgc ggaccgccgg tcctgaagcg atcgtgctgc
      601 ccgagtacgt gctggaattc ggcgaccgcc attatgtcac cgcctactac ggcgacccgg
      661 tggtgggtgc gatccagacg ctgttctccc acccgttcaa ttcccgcatc ctgatccggc
      721 gggatcgctt cgccgccatc ggctgccggg atattccgct cggcaccggc tatgcgtacg
      781 aggactggca cttcaacagc gaagcgctgg cccgcggcat gcgcttcgtc atggcgccgg
      841 ataccgtcct gttctaccgc cgccggccgt ccagcctgtc ggccggttcg cgcagcatct
      901 cgaccggcca gatcccgccc accacgctcg ccgaaccgga aaccgtgcgg acgcgcctcg
      961 ctgccgagta cgccgcgtgg acgcagggct gcgccgcgca gcggcatcgc cggatgctcg
     1021 atcaggtgga gccccgcacg cgcgcgttcc tcgccggccc gctgttccac gaactggcgt
     1081 tcgccgccaa ccggatcgat gccgcggtgg aagccaaccg ctatttcggc acgcatgtct
     1141 ggaccaacct gcgggagccg acgcatatcg ggctggccta ttgccggctc tgcgagatcg
     1201 tcggcaatcg ccggttcacc gacgtgttcc tgctgccgtt cttcgtgcgc ggcggcggcg
     1261 agaaatacct gctcaacgtc atgcacgggc tgatgcaaac ccggccaggg acatcgatcc
     1321 tggtgctgac cggggaaccc cgccagcagc atgactggct ggaccgattg ccggccggtt
     1381 cggcgttcgt cgatctgtgg caggtcggcc ccgactgcac ccgggccgac atcgaactgc
     1441 tgaccctgaa ggcgatccag gcgctggcgc cgtccgcccg catccatctc aagcactgca
     1501 cgtatgcgtt tcaattctgg aaccggttcg ggcgcgtgct ggccgactcc aaccgcggca
     1561 ttctctaccg gtttctcgat acctgttatc aggaccacgg gcggcgcttc ctgggcggct
     1621 tcgaattcga cttcatctcg aacaatctcg acacgttcca tgcgatcgtt acggatcacc
     1681 ggaagattat cgacgccgac cgccagcgct tcgaccaccg ccacgaagtg tgggattggc
     1741 tgcccaccga atgtcagccc acccgctcac cggaacagct ggaggcggcc ccctccggct
     1801 ggcgccggcg cctgctctgg gcctcgcgcc tcgacgatca gaagcgtccg catctgttgc
     1861 cgttgctggc cgccgcgctg cggcgccgcg gcatccagat cagcatcgat gtgtacggcg
     1921 ccgcggtgct gcagaattac gacgtcgcgg cgctgtcctg cgagggcctg tgctatcgcg
     1981 gcgcgttcga agcgttcgaa gcgctggact gcgaggccta cgatggcttc atctacacgg
     2041 cggaattcga tggcctgccg aacgtggtgc tggaggcgct gggagcggga ctgccggtga
     2101 ttgcgccgga tatcggcggc atcgccgaag tcgtccagcc cggccagaca gggtggctgc
     2161 tgccatccga tggctccgaa gcagcgttgg tggatggctt ctgcgacgcg atcgaggcct
     2221 tgtactccga tccgcaacgc cagcgccgga tggcggtcgc cgcagtccgt ctgatcatgt
     2281 ccaggcacgg ccgcacggcg ttcttgcggc ggatcgagcg gatttttgga gactgacggt
     2341 ggcgaccgag cgattgcaca tgagcggcga acagccgtac cacggtttcg aggccgccgc
     2401 gcatgtcgcg cgctatgcgc tgctccggcc ggcttgtgcc cagcggcggg tgctcgatat
     2461 cgcctgcggt gagggatacg gctccgcgct gctggccggc tggggcgcgc ggtccgtcgt
     2521 cggcgtggac atctccgccg cggcaatcgc ctcagcgga
//
LOCUS       DASSZH010000029         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1074310, whole genome shotgun sequence.
ACCESSION   DASSZH010000029 DASSZH010000000
VERSION     DASSZH010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074310"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..586
                     /locus_tag="VFN42_00505"
     CDS             50..586
                     /locus_tag="VFN42_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HET8995123.1"
                     /translation="MKRLFYLLPLAAFLVVAGYFMISLRAGPDVHELPSAMLNKPAPA
                     FDLASLSGGEKLDLDALKGHPFIVNFFASWCVPCRIEHPVLMRLSQQNHLPLYGIAYK
                     DRPEDTKKLLDTYGDPFRLVGLDQSGNVGLNFGVYGVPETYVIDATGRIRRRFVGPLT
                     AETVNKELLPLLKQLDKS"
     gene            583..1095
                     /locus_tag="VFN42_00510"
     CDS             583..1095
                     /locus_tag="VFN42_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HET8995124.1"
                     /translation="MSSGPTQNRAGGAKHLRAALGLLALLLLGFATANAVEPNERLAN
                     PALEARARAISSQLRCLVCQNESIDESAAGLAHDIRIFVRERLVAGETDKQVVQDVVN
                     RYGTFVLLDPPVQPATYVLWYGPPALLLVGLLATVIWVRQRHARPAAEPPLSTEEQQR
                     LDGLLRESDG"
     gene            1092..>2036
                     /gene="ccmI"
                     /locus_tag="VFN42_00515"
     CDS             1092..>2036
                     /gene="ccmI"
                     /locus_tag="VFN42_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HET8995125.1"
                     /translation="MILSLLLAVMAFAAALPLLAPLMRANRPVLERASYDQAVYRDQL
                     QELDRDIARGLLTDAEAESARLEIQRRLLAADRQPAARTRLARSPVLAAIVFVFVTGG
                     AVASYLWLGAPDVPDEPFAGRKAELARDNPQMSLQKAAEQLAAKLKQNPSDADGWLLY
                     GRSLSELGDWDKAADAYGRAIALGRTDPAIVGAHAEMLVLAAGGTVTPAAEDAFKDLL
                     KTDPQNPIARYYLAIALMQAGEPKQAIDGLQALLAELPADSPMRSQIGQAVAQAAQQA
                     GIPTPALAQGAAPAAGPDAAAMAGAAKMTDQQRATMIRG"
BASE COUNT          340 a          691 c          663 g          342 t
ORIGIN      
        1 ccgacggatg ccgcaggcaa tcccggcggc cggggaataa cgatacacga tgaaacgcct
       61 gttctatctc ttgccgttgg cggcattcct ggtcgtagcg ggatatttca tgatctcgct
      121 tcgcgccgga cccgatgtgc atgaactgcc atcggccatg cttaacaagc cggcaccggc
      181 attcgacctt gccagcctga gcggcggcga gaagttggat ctggatgcgc tgaagggcca
      241 tccgttcatc gtcaacttct tcgcctcctg gtgcgtgccc tgccggatcg agcatccggt
      301 gctgatgcgc ctgtcgcagc agaaccacct gcccctgtat ggcatcgcct acaaggaccg
      361 gccggaggat accaaaaagc tgctggacac gtacggcgac ccctttcggc tggtcgggct
      421 ggaccagagt ggcaacgtcg gcctgaattt cggcgtctac ggggtgcccg aaacctacgt
      481 gatcgacgcc accggccgta tccgccgccg cttcgtcgga ccgctgaccg ctgaaaccgt
      541 gaacaaggag ctgctgccgt tgctcaagca gcttgacaag tcatgagcag cggacccacc
      601 caaaatcgtg ccggcggcgc gaaacacctg cgcgccgcgc tcggcctcct cgcgctgctc
      661 cttctcggct tcgccaccgc caacgccgtt gagcccaatg aacggctggc caatccagcg
      721 ctggaagccc gtgcccgcgc catcagctcc cagttgcgct gtctggtgtg ccagaacgag
      781 tcgatcgatg aatccgccgc cgggctggcg catgatatcc gcatcttcgt ccgtgaacgg
      841 ctggtggcgg gtgaaaccga caaacaggtc gtgcaggacg tggtcaaccg gtacggtact
      901 ttcgttctgc tggacccgcc ggtgcagccc gccacgtatg tactttggta tggcccgccg
      961 gcactgctgt tggtcgggct gctcgccaca gtgatctggg tgcgccagcg tcacgccagg
     1021 cctgccgcgg agccaccgtt gagcaccgag gaacagcagc ggttggacgg gcttttgcgg
     1081 gaatccgacg ggtgatcttg tcccttcttc ttgcagtgat ggctttcgcc gcggcgctgc
     1141 ccctgttggc gcccttgatg cgcgccaacc ggccggtgct ggaacgcgcc agctacgacc
     1201 aggctgtgta ccgggaccag ttgcaggaac tcgaccgcga tatcgcccgc ggcctgctga
     1261 ccgacgctga agccgaaagc gcccggctgg agattcagcg ccgtctgttg gccgccgacc
     1321 ggcagccggc cgcccgcacg cgactcgcgc gcagcccggt gcttgcggcg atcgttttcg
     1381 tcttcgtcac cggcggcgcc gtggccagct atctgtggct tggcgcgccc gatgttccgg
     1441 acgaaccctt cgcaggccgc aaggccgagc tggcgcgtga caacccgcag atgtcgttgc
     1501 agaaggcagc cgagcagttg gcggcgaagc tgaaacagaa cccgtccgac gcggatggct
     1561 ggctgctgta tggccgcagc ctgtccgaac tcggcgattg ggacaaagcc gccgacgcct
     1621 acggccgagc catcgcgctg ggccggacag accccgcgat cgtcggcgcg catgccgaga
     1681 tgcttgtcct cgcggccggc ggcaccgtga ccccggcggc cgaggacgca tttaaggacc
     1741 tgctgaaaac cgatccgcaa aacccgattg cgcgctacta cctggccatc gccctgatgc
     1801 aggctgggga gccgaagcag gcgatcgacg gcttgcaggc cctgctggcc gagttgcccg
     1861 ccgactcgcc aatgcgcagc cagatcggtc aggcggtggc acaggccgcg cagcaggcgg
     1921 gtattcccac gccggccctg gcgcagggag ccgcgccggc agccggcccc gacgccgcgg
     1981 ccatggccgg tgccgccaag atgacggatc agcagcgggc gacgatgatc cgcggc
//
LOCUS       DASSZH010000030         3127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1006288, whole genome shotgun sequence.
ACCESSION   DASSZH010000030 DASSZH010000000
VERSION     DASSZH010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006288"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..562
                     /locus_tag="VFN42_00520"
     CDS             77..562
                     /locus_tag="VFN42_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HET8995126.1"
                     /translation="MLWLALGALTLFAFLGGLRDFERASVTTIKSFFAWLLALGGLAL
                     GLLLVLTGREGIALGAVILFGPMLWNYWKAAHPTAGPAPGGPPPGSGAPPPRSPGGPM
                     TREEAYQVLGLSPGASETEIRAAHRRLMRGAHPDQGGSDWLAARINQARDVLLAGTRR
                     A"
     gene            672..1541
                     /gene="galU"
                     /locus_tag="VFN42_00525"
     CDS             672..1541
                     /gene="galU"
                     /locus_tag="VFN42_00525"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010667982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HET8995127.1"
                     /translation="MIKPLRKAVLPVAGLGTRFLPATKATAKEMLPVVDKPLIQYAIE
                     EARAAGIEQFCMVTGRGKTALVEHFDVAFELEHTLRERNKTAALALLEATQVEPGSMV
                     TVRQQVPLGLGHAIWCARAFIGDDPFAILLPDDLVLADTPCLQQLADAYRQTGGNIVA
                     VTEVPPEHTNRYGILKTGKDDGRLVEVQGLVEKPAPKDAPSNLSIIGRYVLMPEVIGH
                     LSRMERGAGGEVQLTDGMARLIGQQPFHGLRYEGRRFDCGDKLGFLEAQIAYALKHPD
                     LAAGVREFLKHYT"
     gene            1557..2966
                     /locus_tag="VFN42_00530"
     CDS             1557..2966
                     /locus_tag="VFN42_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019090204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HET8995128.1"
                     /translation="MTFMHSFDPTTLREYDIRGTVGKTLHAADAFAIGRVFGTILARN
                     GGRTCAVGYDGRLSSPELEAALVQGLSASGIEAVRIGRGPTPMLYFAATTLPTDGAIM
                     VTGSHNPPDYNGFKMMIGRKPFFGDDIKRLGEMAAAGDVDAAHAAVERSTDVSAEYIA
                     RLLADWDGGDRLLKVVWDNGNGAAGDVLQRLVASLPGEHVVLNGSIDGRFPVHHPDPT
                     VPKNLEQIIAEVRARKADIGIALDGDADRIGVIDDTGEIMFGDQLMVLLARDVLKQHP
                     GATIIADVKASQVLFDEIGKAGGSPLMWRTGHSLIKSKMAETGSPLAGEMSGHIFFAD
                     HWYGFDDALYAAVRTLGIVARMQGRLSEVRKALPQVVNTPEVRFDCDDRRKFAVIQEV
                     AERLRAEGAKVSDVDGVRVNTEDGWWLLRASNTQAVLVARAEAKTPEGLERLKAALVR
                     QLEASGLPAPDFSGSHAGH"
BASE COUNT          496 a         1081 c         1086 g          464 t
ORIGIN      
        1 cctgcagacg gtgtgacgcg tcaaagcgtc cggcgtaacc gttgcacagc gtccccgacc
       61 cgcgcagatt gccgccatgc tctggctggc gctcggcgct ctgaccctgt tcgcgttcct
      121 cggcgggctg cgcgacttcg agcgcgcctc cgtcaccacc atcaagtcct tcttcgcctg
      181 gctgctcgcc ctcggcgggc tggcactcgg cctgctgctg gtgctcactg ggcgcgaagg
      241 catcgcgctc ggcgccgtca tcctgttcgg gccgatgctg tggaactact ggaaggccgc
      301 gcacccgacc gccggcccgg ctccgggcgg ccccccgccc ggcagcggcg cgccgccgcc
      361 gcgctccccc ggcgggccga tgacccgcga ggaagcctac caggtgctcg gccttagccc
      421 cggtgccagc gaaaccgaaa tccgcgccgc ccaccgccgg ctgatgcgcg gcgcgcaccc
      481 ggaccagggc ggctccgact ggctcgccgc ccgcatcaat caggcgcgcg acgtacttct
      541 cgccgggacg cgacgcgcat gacggcgcac cgggtcgtga ctaaactcgc aggcacgtgt
      601 gccggccctg ccattcgcag ggcgcgattc cggtctatcc gccatgcttt gcctcccagg
      661 gagcaccacc tgtgatcaag ccgctgcgta aagccgtcct gcccgtcgcg ggactgggca
      721 cacggttcct gcccgccacc aaggccaccg ccaaggaaat gctgccggtg gtcgacaaac
      781 cgctgatcca gtacgcgatc gaggaggcgc gggccgccgg catcgagcag ttctgcatgg
      841 ttaccggccg cggcaagacc gcgctggtgg agcatttcga cgtcgcgttc gagctggaac
      901 acaccctgcg cgaacgcaac aagaccgccg ccctggcgct gctggaagcc acccaggtgg
      961 aacccggctc catggtcacc gtgcgccagc aggtgccgct gggcctgggt cacgcgatct
     1021 ggtgcgcccg cgccttcatc ggcgacgacc ccttcgccat cctgctgccg gacgatctgg
     1081 tgctggccga tacgccgtgc ctgcaacagc tcgccgatgc ctaccggcag accggcggca
     1141 atatcgtcgc cgtcaccgaa gtgccccccg agcacaccaa ccgctacggc atcctgaaga
     1201 ccggcaagga cgacggccgc ctggtggaag tgcaggggct ggtggagaag ccggcgccga
     1261 aggatgcgcc atccaacctg tcgatcatcg gccgctacgt gctgatgccg gaggtgatcg
     1321 gccacctctc ccgcatggag cgcggcgccg gcggcgaggt ccagctcact gatggcatgg
     1381 cccggctgat cggccagcag ccgttccacg gcctgcgcta tgagggcagg cggtttgact
     1441 gcggcgacaa gctgggcttt ctggaggcgc agatcgcgta cgcattgaag caccccgacc
     1501 tggccgcggg cgtgcgcgaa ttcctgaaac actatacctg aggcgggcgc gcgctgatga
     1561 ccttcatgca cagcttcgat cccacgacgc tgcgggaata cgacattcgc ggcacggtcg
     1621 gaaaaacgct gcacgctgcc gatgcctttg ccatcggccg ggtgttcggc accatcctgg
     1681 cgcgcaatgg cggccgcacc tgcgcggtgg gctatgacgg gcggctgtcc agcccggagc
     1741 tggaggccgc cctggtgcag ggcctgagcg ccagcgggat cgaggcggtg cgtatcggcc
     1801 gtggtccgac gccgatgctg tacttcgccg ccaccaccct gccgaccgac ggcgcgatca
     1861 tggtcaccgg cagccacaac ccgccggact ataacggctt caagatgatg atcggccgca
     1921 agcccttctt cggcgacgac atcaagcgat tgggcgagat ggcggcggcc ggcgacgtgg
     1981 acgcggcgca cgccgcggtc gaacgcagca cggatgtcag cgccgaatac atcgcccgcc
     2041 tgctggccga ctgggacggc ggcgaccgcc tgctgaaagt ggtgtgggac aacggcaacg
     2101 gcgcggcggg cgacgtgctg cagcggctgg tcgcgtcgtt gccgggcgag cacgtggtgc
     2161 tgaacggcag catcgacggc cgcttcccgg tgcaccaccc ggacccgacg gtgccgaaga
     2221 acctggagca gatcatcgcc gaggtacggg cgcgcaaggc ggatatcggc atcgcgctgg
     2281 acggcgacgc cgatcgcatc ggcgtgatcg acgataccgg cgagatcatg ttcggcgacc
     2341 agctcatggt gctgctggcg cgcgacgtgc tgaagcagca cccgggcgcc accatcatcg
     2401 ccgacgtgaa ggccagccag gtgctgttcg atgagatcgg caaggccgga ggatcgccgc
     2461 tgatgtggcg caccggccat tcgctgatca agtcgaagat ggcggagacc ggctcgccgc
     2521 tggccggcga gatgtcgggg cacatctttt tcgccgatca ctggtacggc ttcgacgacg
     2581 cgctgtacgc ggcggtgcgc acgctgggca tcgtggcccg gatgcagggc aggctgtcgg
     2641 aggtgcgcaa ggcgctgcct caggtggtga acacgccgga ggtgcggttc gactgcgacg
     2701 accgccgcaa attcgcggtc atccaggaag tcgcggaacg cctgcgcgcc gagggcgcca
     2761 aggtgtcgga cgtggacggc gtgcgggtga acaccgagga cggctggtgg ctgctgcgcg
     2821 cctccaacac ccaggcggtg ctggtggccc gtgccgaggc gaagacgccg gaggggctgg
     2881 agcggctgaa agccgcgctg gtccgccagt tggaagcctc cggcctgccc gccccggact
     2941 tctccggcag ccacgcgggg cactgagcgg aaaattcggg cactgaggag aaggtaggga
     3001 ctcctccccc tcccctcgcg gcccaagccc gcgaggggag ggggagcagt ctcgcccgtc
     3061 tgcgccgggc acatggcggc catggcgggg atttcgcttc ttttttccgc catggtcgcc
     3121 aattatg
//
LOCUS       DASSZH010000031         2010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1057418, whole genome shotgun sequence.
ACCESSION   DASSZH010000031 DASSZH010000000
VERSION     DASSZH010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057418"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..147
                     /locus_tag="VFN42_00535"
     CDS             <1..147
                     /locus_tag="VFN42_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET8995129.1"
                     /translation="IAYLSGLVRLAPGDLIFTGTPEGVAAVQRGDLLEGTVEGVGTVR
                     TRIV"
     gene            144..953
                     /locus_tag="VFN42_00540"
     CDS             144..953
                     /locus_tag="VFN42_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET8995130.1"
                     /translation="MSTPFRVATWNINSLRLRATLLKQLVASLDPDVICLQETKVPDE
                     LFPADVPPAIGYPYALFRGMKGYNGVAVLSRRPILLQDGAPDWCGKGDCRHIGVQMDM
                     PGGPVTLHNFYVPAGGDVPDRALNVKFGHKLDFIAETQAWFAAQSGLARSVLVGDLNI
                     APLEHDVWSHKQLLNVVSHTPPETAGLTAWLEAGFVDAVRHFVPPEQKLYTWWSYRNR
                     DWRSSDRGRRLDHVWVTPDLQGGLQRQIVLKDARDWPQSSDHVPVCVELSL"
     gene            1049..1471
                     /locus_tag="VFN42_00545"
     CDS             1049..1471
                     /locus_tag="VFN42_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1489 domain-containing protein"
                     /protein_id="HET8995131.1"
                     /translation="MLHMTKLAVGVRDIEHLREIQADRAERNPPLRHRTRNFPRRREE
                     ILDGGSMYWVVNGAMLVRQRIVDIIEDHWDDNSACAGLLLDPQLVPLAGRPTKPFQGW
                     RYLPPDDAPPDLATTRPVRGEAALPAALRQELRVLCLL"
     gene            1609..1833
                     /locus_tag="VFN42_00550"
     CDS             1609..1833
                     /locus_tag="VFN42_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995132.1"
                     /translation="MTTSRKHLIAALALALPAAAFLAAPASAASTGVTHHKKHHVVHA
                     HHVVRHHHKPLLHKISAHHHVAHSSKKPHA"
BASE COUNT          327 a          712 c          656 g          315 t
ORIGIN      
        1 atcgcctatc tgtccggcct ggtccggctg gcgcccggcg acctgatctt caccggcacg
       61 ccggaaggcg tggcggcggt gcagcggggc gacctgctgg aaggcacggt ggagggcgtc
      121 ggcaccgtgc ggacgcgcat cgtttgagca cgccgttccg ggtcgccacc tggaacatca
      181 actcgctgcg gctgcgggcg acgctgctga aacagctggt ggcatcgctc gaccctgatg
      241 tgatctgcct ccaggagacg aaggtcccgg acgagctgtt cccggccgat gtcccccctg
      301 cgatcggcta tccctacgcg ctgttccgcg gcatgaaggg ctataacggc gtggcggtcc
      361 tgtcgcgccg gcccatcctg ttgcaggacg gtgcgccgga ctggtgcggc aagggcgact
      421 gccggcacat cggcgtgcag atggacatgc ccggcggccc ggtgacgctg cacaatttct
      481 atgtgccggc cggcggggat gtgccggacc gtgcgctgaa cgtgaagttc ggccacaaac
      541 tggatttcat cgccgagaca caggcgtggt tcgccgcgca atccgggctg gcgcgctccg
      601 tgttggtggg cgacctgaac atcgcgccgc tggagcacga cgtctggagc cacaagcaac
      661 tgctgaacgt ggtcagccat accccgccgg aaaccgccgg cctgacggcc tggctggaag
      721 ccgggttcgt tgacgccgtg cggcacttcg tgccgccgga gcagaagctc tacacgtggt
      781 ggtcgtaccg caaccgtgac tggcgcagtt ccgatcgcgg gcggcggctg gaccatgtct
      841 gggtgacgcc ggacctgcaa ggcgggctgc agcgccagat cgtgctgaag gacgcgcgcg
      901 attggccgca gagctccgac cacgtcccgg tctgcgtcga gctgagcctg taggctgggc
      961 cgccgccggc ccggccaagg ccctggcctc gccgcggtga ccaaaggcat actccctgcc
     1021 tgaccaaaac cggtcccgag tcgtccccat gctgcacatg accaagctcg ccgtcggcgt
     1081 acgcgacatc gagcatctgc gtgaaatcca ggcggaccgc gccgaacgca acccgccgct
     1141 gcgccaccgc acgcgcaact tcccgcgccg gcgggaggag atcctggatg gcggctcgat
     1201 gtactgggtg gtcaatggcg ccatgctcgt gcgccagcgc atcgtggaca tcatcgaaga
     1261 ccattgggac gacaacagcg cctgcgccgg cctgctgctc gacccgcaac tggtgccgct
     1321 ggcggggcgg ccgaccaagc cgttccaggg ctggcgctac ctgccgccgg atgatgcgcc
     1381 gcccgatctt gccaccaccc gcccggtgcg gggcgaagcg gcgctgccag cggccctccg
     1441 ccaggagcta cgagttctct gcctgctata ggcggcgaat cggcgtcaag ccgcttgcct
     1501 ccttgtgtca aatatagcat aatgcaaaca gtcgtttagg gcgcaatttt cgcccccgac
     1561 tctaagctga tcccccgcaa cgctctcctg acatggagcc atcccgccgt gaccacctcg
     1621 cgcaaacacc tgattgccgc gctcgcgctt gccctgccgg ccgcggcgtt cctggctgca
     1681 cccgcctccg ccgccagcac cggcgtcacc caccacaaga agcaccatgt cgtgcatgcg
     1741 caccatgtgg ttcgccacca ccacaagccg ctgctgcaca agatcagcgc gcatcaccac
     1801 gtggcgcaca gcagcaagaa gccgcacgcc tgagcgtggc ttccaccgcg gcgaaggcca
     1861 ggaaccgccc ggtccctggc cttttttcat ggccactcag cgccgccgcc gttgagggac
     1921 attggcttcc accagggcgc gcaatccggc cagcagccga gccgggctgg gcgggcaacc
     1981 gctgatcgtc aggtccaccg gcagcacgct
//
LOCUS       DASSZH010000032         2656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_171068, whole genome shotgun sequence.
ACCESSION   DASSZH010000032 DASSZH010000000
VERSION     DASSZH010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171068"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..777
                     /locus_tag="VFN42_00555"
     CDS             148..777
                     /locus_tag="VFN42_00555"
                     /inference="COORDINATES: protein motif:HMM:NF021595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphotransferase family protein"
                     /protein_id="HET8995133.1"
                     /translation="MTGRHDALHLAELLCARMTHDFSGSIGALSNLLDLAFDQPERAA
                     EPLALASETAGQLVRRLKLLRAAWGPPMEPFPLGRIHDLALGLAQSRCCVNLEALAGG
                     TEFSMEHGRLVLNLLLMAADGLPSGGEVALRGCAGDLVLVIAGQRAAWPAGLAACIAD
                     PDSAWSMLEDPRKVQTALTVLLAASLGMRLSLLMGPEAGGVPPLRIRTA"
     gene            900..>2656
                     /locus_tag="VFN42_00560"
     CDS             900..>2656
                     /locus_tag="VFN42_00560"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HET8995134.1"
                     /translation="MDDLLADFLTETNESLAELDLALVTLERTPDDADTLSQIFRLVH
                     TIKGTCGFLGLPRLERVAHAAESVLGKVRDRKLAVTPDVVSQVLVALDRIKAIVAELG
                     STANEPPGDDAALIATLEQIAAGDVPARAPQPAAASVQDMVEEPAPEPQERDDPPEEE
                     SVPVEAAPAAPLPVAAPSAAAPAGPQTIRVTVDVLEDLMTLVSELVLTRNQLLQLARM
                     QENSGFTVPLQRLSHITSDLQEGVMKTRMQPIGNAWNKLPRLVRDLARETGKKIELTM
                     LGADTELDRQVLELIKDPLTHMVRNSADHGLEPPDRRRAAGKPETGRITLNAFHEGGH
                     IIIEVADDGAGLPVDRIRAKAFANGLATEAEIAAMSDGQVQRFIFRAGFSTASVVTAV
                     SGRGVGMDVVKTNIEKIGGTIDLKSVAGQGTTFFIKIPLTLAIVSALIVEAGGERFAI
                     PQISVVELVRAQRQKTEAGDGESVIEHINDTPVLRLRDRLLPLVSLTGLLALDPPAQD
                     DADATIVVVQVGANMLGIIVDCVFDTEEIVVKPVAPILRDITMFSGNTILGDGSVIMI
                     LDPNGIARGAGGAGGATGD"
BASE COUNT          436 a          893 c          908 g          419 t
ORIGIN      
        1 gtctggccgc gcgccggagc tttgcgggaa atcggctatg caatccagga gcccgtaccg
       61 ggcggataaa ccgtttcccg cgactcgggc agatgtggca aaatcatccc ctcgccggag
      121 gatgcctccg gccgggggaa cgtgccgatg accggccgac atgacgcatt gcatctggca
      181 gaattgctct gcgcccgcat gacgcacgat ttcagcggca gcatcggcgc actgagcaac
      241 ctgctcgatc tggccttcga ccagccggag cgggcggccg agccgctggc cctggcaagc
      301 gagacggccg ggcaactggt gcggcgcctg aagctgctgc gcgccgcctg ggggcccccg
      361 atggagccgt ttccgctcgg ccggatacat gacctggcgc ttggcttggc ccagtcccgc
      421 tgctgtgtga atctcgaagc tctggcgggc ggtaccgagt tctcgatgga gcacggccgg
      481 ctggtactga acctgctgct catggcggcg gacgggctgc cctcgggcgg tgaggtcgcg
      541 ctgcgcggct gtgccggcga tctggtgttg gtcattgccg gccaacgcgc ggcttggccg
      601 gctgggctgg cggcatgcat cgccgatcct gacagcgcgt ggtccatgct ggaggatccc
      661 cgaaaggtgc agaccgcgtt gacggtgctg ctggccgcct cgctgggtat gcgcctgtcg
      721 ttgttgatgg ggccggaggc tggcggcgtt ccgccgctgc ggatccgtac agcgtagcgc
      781 cgcggcagat ttcccccgca cgcgtggggg cgtttccaaa cagattcccg cccggcaatt
      841 taccctttgt tcgtcatctt cgatgcagga tcaccccgcc ggacaccgag ggggtgctca
      901 tggacgacct gctggccgat ttcctcaccg agaccaacga gagcctggcc gaactggacc
      961 tggccctggt cacgctggag cggacgccgg acgacgccga caccctgtcg cagatttttc
     1021 ggctggtgca caccatcaag ggcacctgcg gtttcctggg cctgccgcgg ctggaacgcg
     1081 tggcgcacgc cgcggagagc gttctgggca aggtgcgtga ccgtaagctg gcagtgacgc
     1141 cggacgtcgt cagccaggtg ctggtcgcgc tcgaccggat caaggcgatc gttgccgaac
     1201 tcggcagtac cgccaatgag ccgcccggtg acgacgcggc actgatcgcc acgctggagc
     1261 agatcgccgc cggcgacgtg ccggcacgcg ctccccagcc ggccgcggcg agcgtgcagg
     1321 acatggtgga agaaccggca cccgagccgc aggagcgcga tgacccccca gaggaggaat
     1381 cggtgccggt ggaagctgct ccggccgctc cccttcctgt cgccgcgccc agtgccgccg
     1441 cgcctgccgg gccgcagacc attcgcgtca ccgtcgacgt gctggaagac ctgatgacgc
     1501 tggtcagcga gctggtgctg acccgcaacc agcttctgca gcttgcccgc atgcaggaga
     1561 acagcggctt caccgtcccg ctgcagcgcc tgtcgcacat cacctccgac ttgcaggagg
     1621 gggtgatgaa gacgcgcatg cagccgatcg gcaatgcctg gaacaagctg ccacggctgg
     1681 tgcgcgacct tgcccgcgaa accggcaaga agatcgaact caccatgctt ggcgccgaca
     1741 ccgagctcga ccggcaggtg ctggaactga tcaaggaccc gctcacccac atggtgcgca
     1801 acagcgccga ccatgggctg gagccgccgg accggcgccg agccgcgggc aagccggaaa
     1861 ccgggcgcat caccctgaac gcgttccacg aaggcggcca catcatcatc gaggtcgccg
     1921 atgacggcgc cggcctgccg gtggacagga tccgcgccaa ggccttcgcc aacggtcttg
     1981 ccaccgaggc cgaaatcgcc gcgatgagcg acggccaggt gcagcgcttc atctttcgcg
     2041 ccggcttctc caccgcatcg gtggtcaccg cggtatccgg ccgcggcgtc ggcatggacg
     2101 tggtgaagac caatatcgag aagatcggcg gcaccatcga cctgaagagc gtcgccggcc
     2161 agggcaccac gttcttcatc aagattcccc tgaccctggc catcgtctcc gcgctgatcg
     2221 tcgaggccgg tggcgagcgc ttcgccatcc cgcagatcag tgtggtggaa ttggtgcgcg
     2281 cgcagcgcca gaagaccgag gcgggcgacg gcgagtcggt gatcgagcat atcaacgaca
     2341 cgccggtgct gcgcctgcgc gaccggttgc tgccgctggt cagcctgacc ggcctgctgg
     2401 cgctggaccc gcccgcgcaa gacgacgccg atgcgaccat cgtcgtggtg caggtcggcg
     2461 ccaatatgct gggcatcatc gttgactgcg tgttcgatac cgaggagatc gtggtgaagc
     2521 cggtcgcgcc gatcctgcgc gacatcacga tgttcagcgg caacaccatc ctgggcgacg
     2581 gatcggtgat catgatcctc gatccgaacg ggatcgcccg cggtgccggc ggcgccggcg
     2641 gcgccaccgg cgacaa
//
LOCUS       DASSZH010000033         2607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_290409, whole genome shotgun sequence.
ACCESSION   DASSZH010000033 DASSZH010000000
VERSION     DASSZH010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290409"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VFN42_00565"
     CDS             <1..210
                     /locus_tag="VFN42_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995135.1"
                     /translation="LAELRGQAGRIPDAALLAALDAAVAVAPDHTWHGPAVRLFANWL
                     SYLAQRKAARDAAALWPLRQRRVAS"
     gene            346..1341
                     /locus_tag="VFN42_00570"
     CDS             346..1341
                     /locus_tag="VFN42_00570"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HET8995136.1"
                     /translation="MRETTLRTPPHGAAALHALFARTARRSENVILPINDGALGTGNE
                     RPAFYCVHSLSGAGGTDFRELAKLMPDVRFYGIQAPTTKMQDAEFGRSVELLADHYAD
                     ALVRFQPDGPFLLGGWSAGATIALEIAQTLLHRGREVRLLAAIDAAPENTGAGLRPWH
                     PLYLLELAANLPGWVRHDLRKTSLRALVRRVSARITAAGKKAVIRDMGKPGLDLGFAV
                     EGFVDLSRYPPDQKSFMKRLYNAVMEYNAVHYSEQVVVYEAKVKPLSHLPQVGAVWRK
                     LAPRSVVIRVDGTHLSIMREGYVHVIAEDLRKRVTEGWSDAARGPLPIEPHPAGR"
     gene            complement(1447..1521)
                     /locus_tag="VFN42_00575"
     tRNA            complement(1447..1521)
                     /locus_tag="VFN42_00575"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1487..1489),aa:Val,seq:gac)
     gene            1668..>2607
                     /locus_tag="VFN42_00580"
     CDS             1668..>2607
                     /locus_tag="VFN42_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HET8995137.1"
                     /translation="MPFDVREFPAGAEAVARNRRAVGGWLLGVAFMVFVMVVLGGATR
                     LTGSGLSIMEWAPLRGALPPLNEAEWQRLFHLYQHIPQYTLLHDGFGLAGFKRIFWLE
                     WTHRLWGRLVGLAFLLPLIWFWARGAIERRLRPRLVLLFLLGGLQGAVGWFMVASGFF
                     PDSTTVSPYRLVVHLSFALVLYAVLLWTGLTVLRPTPVPAPAAPRRLLQWVVALVGLT
                     IVAGGFTAGLHAGKVYNTFPLMDERLVPAGYADLSPFLRNLTENVAAVQFDHRLLATA
                     TALLAAATAVIGLLRAPPPPLRNALVALGAMVGVQYA"
BASE COUNT          366 a          890 c          883 g          468 t
ORIGIN      
        1 ctggcggagc tgcgcgggca ggccgggcgg attccggacg cggccctgct tgccgccctg
       61 gacgccgccg tcgcggtggc gccggaccat acctggcacg ggccggctgt gcggctgttc
      121 gcaaactggc tgtcctatct ggcacagcga aaggctgcgc gcgacgcagc ggcgctatgg
      181 cccctgcggc aacggcgggt ggcatcgtga gcagttgcgc cctctttcca ccacgcgacc
      241 agcggcggga acgggatgat cgcctgacgc ggcagtcgca gcactggagg aggcaagggc
      301 agcgtgcctc cggtccggca accatcgcca gccgtaggac cgcccatgcg tgaaacgacc
      361 ctcaggactc cgccgcacgg tgccgcggcg ttgcatgcct tgtttgccag gacggcacgc
      421 cgctcggaga acgtgatcct gccgatcaac gacggcgctc tcgggacagg caacgagcgg
      481 ccggccttct actgtgtgca ttccttgtcg ggggccggcg ggacggattt ccgtgaactc
      541 gccaagctga tgccggatgt ccgtttctac ggcattcaag cgccgaccac gaagatgcag
      601 gatgcggaat tcggccgctc ggtcgaattg ctggcggatc attatgccga cgctcttgtc
      661 cggttccagc cggacggccc gttcctgctg ggcggatggt ccgcgggcgc gacgatcgcc
      721 ctggaaatcg cgcagacgct gctccaccgg gggcgtgagg ttcgactgct cgccgcgatc
      781 gacgcggcgc ctgaaaacac cggtgccggg cttcggccct ggcatccgct ctacctgctg
      841 gaactggccg ccaatctgcc cggctgggtc cggcatgacc tgagaaaaac ctccctccgg
      901 gcgctggtgc gacgggtttc ggccaggatc accgccgctg gcaaaaaggc cgtgattcgc
      961 gatatgggca agcccggact ggaccttggc tttgctgtcg agggattcgt ggatctctcc
     1021 cgctatccgc ccgaccagaa atcgttcatg aaaagactct ataatgcggt aatggaatat
     1081 aacgcggtgc attattcgga gcaggtggtc gtgtatgagg caaaggtgaa gccactctcc
     1141 catttaccac aggtaggcgc tgtctggcgc aagctggcac cacgatcggt ggtcattcgc
     1201 gtggatggaa cgcatctgag catcatgcga gagggctacg tgcatgtgat tgccgaagac
     1261 ctgcgcaagc gggtcaccga gggatggtcg gacgctgcac gcggtccgct gcccatcgaa
     1321 ccgcatcccg cggggcgctg atcattcccg cggagtggct ggaccacccc tgcgctgctt
     1381 ccgcctggcg tgcgcgacct tgccccgccc gccaccttac cgacggggta caaccccttg
     1441 atttcgtggt gggcgcgact gggattgaac cagtgacccc caccgtgtca aggtggtgct
     1501 ctcccactga gctacgcgcc cacaaaccgc gtcggcgcgg tgttggtagg cgcaccgcgc
     1561 accgcatgca agcccttcca tgcggcggcg cacccctgcg tgagaggggg cgtccgtttc
     1621 gcaggccggc gctgtttccc ggcgcgtcgc ttctatatca tgcgccgatg ccgttcgatg
     1681 tccgcgagtt ccccgccggt gccgaggccg tggcacgcaa ccgccgtgcc gtcggcggct
     1741 ggctgctcgg cgtcgccttc atggtgttcg tcatggtcgt gctgggcggc gccacgcgcc
     1801 tgaccggctc cggcctgtcg atcatggaat gggcgcccct gcgcggcgcc ctcccgccgc
     1861 tcaacgaggc ggaatggcag cggctgttcc acctctacca gcacatcccg caatacacgc
     1921 tgctgcatga cgggttcggg ctggctgggt tcaagcgcat cttctggctg gaatggacgc
     1981 accggctgtg ggggcggctg gtcggactcg ccttcctgct gccgctgatc tggttctggg
     2041 cgcgcggcgc gatcgagcgg cggctgcgcc cgcgtctcgt gctgctgttc ctgctcggcg
     2101 ggctgcaggg cgcggtcggc tggttcatgg tcgcctccgg tttcttcccg gacagcacca
     2161 ccgtctcccc ctaccgcctc gtggtgcatc tcagcttcgc gctggtgctg tatgcggtgc
     2221 tgctgtggac cgggttgacg gtgctgcgcc cgacccccgt gcccgcgccg gcggcgccgc
     2281 ggcggctgct gcagtgggtc gtggcactgg tcggcctcac catcgtcgcc ggcggcttca
     2341 ccgccggact gcacgccggg aaggtctaca acaccttccc gctgatggac gaacggctgg
     2401 tgccggcggg ctatgccgac ctctcgccgt tcctgcgcaa cctgacggaa aacgtcgccg
     2461 cggtgcagtt cgaccaccgc ctgctggcga ccgcgaccgc cctgctcgcc gccgcgaccg
     2521 cggtgatcgg cctgctgcgc gcgccgccgc cgccgctgcg caacgccctg gtggcactcg
     2581 gcgccatggt cggcgtgcaa tatgcgc
//
LOCUS       DASSZH010000034         5136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_85867, whole genome shotgun sequence.
ACCESSION   DASSZH010000034 DASSZH010000000
VERSION     DASSZH010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85867"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /gene="moaB"
                     /locus_tag="VFN42_00585"
     CDS             complement(<1..522)
                     /gene="moaB"
                     /locus_tag="VFN42_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013641037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HET8995138.1"
                     /translation="MARIDESRPFIPVNIAVLTVSDTRTAANDTSGDALAQRIEHSKH
                     RVAARAIEQDDAGKIETLLRRWIDDPQIDVVITTGGTGITGRDVTPEAFDRVLEKQIE
                     GFGELFRMLSYQKIGTSTMQSRAVGGVAHGTYLFALPGSTGAVKDAWDDILVAQLDNR
                     HRPCNLVELMPRLQ"
     gene            complement(555..2213)
                     /locus_tag="VFN42_00590"
     CDS             complement(555..2213)
                     /locus_tag="VFN42_00590"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET8995139.1"
                     /translation="MPQPLDPSDAALVRRVFALQDHGKLTEADRLLHGVQNRLLLGEI
                     LADRYLGPYHRSTVAELQDWLKHYAELPDAAAIRALLLRRAGPGTTAPPLPPPQDLAL
                     PHKLPAQPAEDPDPEPGVTRNPALDRAVASRLEHGEPQSALRLIAATHHLSPSYGVLL
                     RAEVARALFAVNDNVEALRLAVEAEHAVPMKDRVGRGYYVAGLAAWRLGRVESARTLF
                     EAAARAPVSTAAIRAGAAFWTSRADLRLHARAAALQWMRKAALEHRTLYGLLARRVLG
                     LHTGIIPSGSLLTQADVDAVAAVPAGDRAFALLEVGETRRAEAELREAWAQVKDDPGM
                     RRSVLLVAASAGLRDLAGHFAMLEAEADGTQPAAPSELPRLRPAHGFRIDPALVYAVT
                     RMESNFNANAVSAAGARGLMQIMPVTARYVAGNPSLSSAELHNPGLNLALGQRYLRWL
                     AQQSVVDDNLLYLLASYNAGAGNLAGWLRGMHDQGDPLLFLEAIPVPETRAFVTEVLA
                     YTWRYAARLHVPAPSLDALAAGEFPRFTPLAKRSKLQTAKTQTD"
     gene            complement(2515..3315)
                     /locus_tag="VFN42_00595"
     CDS             complement(2515..3315)
                     /locus_tag="VFN42_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET8995140.1"
                     /translation="MDDYALLTLQIAWGADEALDDAPVDRLRTAAPVRPAPAHSVPPA
                     PARPLQARPPAAAPGLPSQTTAERAVAAAGSANSIEALASAIAGFDGCVLRDTATRAV
                     LPQGDAASGLLLIGDPPGADEDRSGQPFAGEDGALLDRMLASVGLDRAGLLMAPLIPW
                     RPPGGRPPSAGELAICLPFLHRLIVLAEPRQILLLGPLAVRMLLDGKRRRPGSGWTTL
                     SLPGLPTPVPALVLPTPHMVRSTPSVRRDAWTELRRLRRALDGGIAEL"
     gene            3432..5069
                     /locus_tag="VFN42_00600"
     CDS             3432..5069
                     /locus_tag="VFN42_00600"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HET8995141.1"
                     /translation="MAEDLPPREAMEFDVVIVGGGPSGLAAAIRLKQLAPDAAVCLVE
                     KGSEIGAHILSGAVLEPRALNELLPDWKEQGAPLETPATDDRFLFLTETKAYRLPTPP
                     QMDNHGNYIVSLGNVCRWLGQQAEALGVEIYPGFAAAEVLEEDGRVVGVATGDMGIGK
                     NGEPGPNFQRGMELRARYTLLAEGCRGSLSKRLIARYNLRQGHDPQTYAIGIKELWEI
                     PAENHKAGLVEHTVGWPLDSRTYGGSFLYHLGSNLISYGFVIGLDYSNPWLSPFEEMQ
                     RFKTHPEVRKHFAGGRRISYGARALNEGGWQSIPDLVFPGGLLIGDAAGFVNVPKIKG
                     THTSMKSGMLAAEAVAEALAGGKPAELTSYPEKLRASWVWEELHGVRNIRPSFAKFGM
                     WGGLAYSALDTYVFRGKAPWTMHHPHADNDTLVPAEQAPRIAYPKPDGTLTFDRLSSV
                     FISNTNHEEDQPAHLRLRDPDLAITTNWDRFRSPETRYCPAGVYEIVGADEGTPKLQI
                     NAQNCVHCKTCDIKDPGQNIDWVTPEGGGGPNYPGGM"
BASE COUNT          797 a         1818 c         1777 g          744 t
ORIGIN      
        1 ctgcaaccgc ggcatcagtt ccaccaggtt gcagggccgg tgccggttgt ccagctgcgc
       61 caccaggatg tcgtcccagg cgtccttcac cgcgccggtg ctgccgggca gtgcgaacag
      121 gtaggtgccg tgcgccaccc cgcccaccgc gcgcgactgc atcgtcgagg tgccgatctt
      181 ctggtagctc agcatgcgga acagctcgcc gaacccctcg atttgcttct ccagcacccg
      241 gtcgaacgcc tccggcgtca cgtcccgtcc ggtgatgccg gtgccgcccg tggtgatcac
      301 cacgtcgatc tgcgggtcgt cgatccagcg ccgcaacagc gtctcgatct tccccgcgtc
      361 atcctgctcg atcgcccggg cggccacccg gtgcttcgaa tgctcgatcc gctgtgccag
      421 cgcgtcgccg gaagtgtcgt tcgccgcggt gcgtgtgtcc gataccgtca ggaccgcgat
      481 gttcaccgga atgaacgggc ggctttcatc gatgcgtgcc atgctgcgcc tcctgccgtt
      541 gcggccgcag tccttcaatc ggtctgcgtc ttggcggttt gcagtttgct cctcttggct
      601 agcggcgtga agcgcgggaa ctccccggcg gccagggcgt ccaggcttgg cgccggcaca
      661 tgcaggcggg cggcataccg ccaggtatag gccagcacct cggtgacgaa ggccctggtc
      721 tccggcaccg ggatcgcctc gaggaacagc agcgggtcgc cctggtcgtg catgccgcgc
      781 agccagcccg ccaggttgcc ggctcccgca ttgtagctcg ccagcaggta tagcaggttg
      841 tcgtcaacca cgctctgttg cgccagccat ctcaggtagc gttgccccag cgccaggttc
      901 agccccggat tgtgcagctc cgcgctggac agcgaaggat tgccggccac atagcgggcg
      961 gtcaccggca tgatctgcat cagcccgcgc gcccccgcgg ccgacaccgc attggcgttg
     1021 aaattggatt ccatgcgcgt caccgcatag accagtgccg gatcgatgcg gaagccatgc
     1081 gccggccgca gccgcggcag ctccgagggg gccgccggct gggtgccgtc cgcctctgcc
     1141 tccagcatgg cgaaatggcc cgccaggtca cgcagtccgg ccgaggcggc gaccagcagc
     1201 accgaccggc gcatccccgg atcgtccttc acttgcgccc aggcctcgcg caattccgcc
     1261 tcggcgcgtc gggtctcgcc aacttccagc agggcgaagg cgcggtcgcc ggccggcacc
     1321 gccgccaccg catccacgtc cgcctgggtg agcaggctgc cgctggggat gatcccggtg
     1381 tgcaggccaa gcacccgccg ggccagcagc ccatacagcg tgcgatgctc gagtgcggcc
     1441 ttgcgcatcc attgcagcgc cgccgcgcgg gcatgcagcc gcaggtcggc gcgggaggtc
     1501 cagaacgcgg ccccggcgcg gatcgcggcc gtgctgaccg gggcacgggc ggcagcctcg
     1561 aacagggtgc gggcggactc cacgcggccc agccgccagg cagcgagccc ggcgacatag
     1621 tagccgcggc cgacccggtc cttcatcggt accgcgtgtt ctgcctccac cgcgaggcgc
     1681 agcgcctcga cgttgtcatt gacggcaaac agcgcgcggg cgacctcggc gcgcagcagc
     1741 acgccatagg acggcgacag atggtgggtg gcggcgatca gccgcagcgc cgattgcggc
     1801 tcgccatgct ccagccgcga tgccaccgcg cggtccagcg cgggattgcg ggtcacgccg
     1861 ggctccgggt caggatcctc ggccggctgg gcgggcaatt tgtgcggcag cgcaaggtcc
     1921 tgtggtggcg gcagcggcgg cgccgtggtg ccgggaccgg cccggcgcag cagcagcgcg
     1981 cggatcgctg cggcgtccgg cagctcggcg taatgcttca gccagtcctg cagctccgcc
     2041 acggtcgagc ggtgatacgg ccccaggtag cggtccgcca gaatctcccc cagcagcagc
     2101 cggttctgca cgccatgcag caggcggtca gcctctgtca gcttgccgtg gtcctgcagc
     2161 gcgaacacgc gccggaccaa ggccgcgtcg ctggggtcca gcggctgcgg caacgcgact
     2221 tccgtggcgg tgcgcagcac cacccgcgga aacgcaaacg cggtttgatc gtccgccgca
     2281 ttcccagacc ctggcgcttg cgcctgtgct cccgagacga gcgtcccgat gaggatggca
     2341 gcgccgaaaa cacactggat cgccctagga atgcgggaga gagtgtgagt cacccctcgc
     2401 atgcggtgac cggctaccgg tgcccgccgg agaaggcaat cccctatctc tgagacccgt
     2461 ctcaataccg agacggttct gtagggaatc agtaacttag acttcagccc gttttcacaa
     2521 ttcagcgatc ccgccgtcca gcgcccgccg cagccgccgc agctcggtcc aggcgtcacg
     2581 ccggacactg ggtgtggacc gcaccatgtg cggcgtcggc agcaccagcg ccggcaccgg
     2641 ggtgggcagg cccggcaggc tcagcgtggt ccagccgctg ccgggccgcc gccgcttgcc
     2701 gtccagcagc atgcgtacgg cgagcggacc cagcagcagg atctgacgcg gttcagccag
     2761 cacgatcagg cggtggagga agggcaggca gatcgccagc tcgcccgcgc tgggcggccg
     2821 gccgccgggc ggacgccagg ggatcagcgg cgccatcagc agcccggcgc ggtctaggcc
     2881 gacgctcgcc agcatgcggt ccagcagcgc cccgtcctcc ccggcgaagg gctggccgga
     2941 gcggtcctcg tcggcgccgg gcggatcgcc gatcagcagc aggccggacg cggcatcacc
     3001 ctgcggcagg accgcccggg tggcggtatc gcgcagcacg cagccgtcga acccggcaat
     3061 ggcactggcc agcgcctcga tgctgttcgc cgagccggcg gcggcaacgg cgcgttccgc
     3121 cgtggtttgg gatggcaggc ccggtgcggc ggcgggcggg cgtgcctgca gcggccgcgc
     3181 tggcgcgggc ggcaccgaat gtgccggcgc cggccgcact ggcgcggccg tccgcagccg
     3241 gtccaccgga gcgtcgtcca gcgcctcgtc ggcgccccat gcgatctgca gggtcagcag
     3301 agcgtaatcg tccatggccc agtcctgcac gcggcggccc gcggtgcaac tgggttcagg
     3361 cggcggcccc cgtgtgacag ccccgtcgtt ccaccctagt ttgcgccccc acagtcagga
     3421 ggacgcagca catggcggag gacctgcccc cgcgcgaggc gatggaattc gacgtggtga
     3481 tcgtcggcgg cggcccgagc ggcctggctg ctgccattcg cctcaagcag ctcgcgccgg
     3541 atgccgctgt gtgcctggtg gagaaaggca gcgagatcgg tgcgcatatc ctgtccggcg
     3601 cggtgctgga accgcgcgcg ctgaacgagc tgctgcccga ctggaaggag cagggcgctc
     3661 cgctggagac gcccgccacc gacgatcgct tcctgttcct gaccgagacc aaggcgtacc
     3721 gcctgcccac cccgccgcag atggacaacc acggcaacta catcgtctcc ctcggcaatg
     3781 tctgccgctg gctcgggcag caggcggaag cgctgggcgt ggagatctat cccggcttcg
     3841 ccgccgccga ggtgctggag gaagacggcc gcgtcgtcgg cgtcgccacc ggcgacatgg
     3901 gcatcggcaa gaacggcgaa cccggcccca acttccagcg cggcatggag ctgcgcgccc
     3961 gctacacgct gctcgccgag ggctgccgcg gctcgctctc caagcgcctg atcgcccgct
     4021 acaacctgcg ccagggacac gatccgcaga cctacgccat cggcatcaag gaactgtggg
     4081 aaatcccggc ggagaaccac aaggccgggc tggtggaaca caccgtcggc tggccgctgg
     4141 acagcaggac ctatggcgga tcgttcctct accacctcgg cagcaacctg atctcctacg
     4201 gcttcgtcat cggcctggac tacagcaatc cgtggctgtc gccgttcgag gagatgcagc
     4261 gcttcaagac gcatccggag gtgcgcaagc actttgccgg cgggcgccgc atcagctacg
     4321 gcgcacgcgc gctgaacgag ggcggctggc agtcgatccc ggatctggtg tttcccggcg
     4381 ggctgctgat cggtgacgcg gccggcttcg tcaacgtgcc gaagatcaag ggcacgcata
     4441 cctcgatgaa gtccggcatg ctggcggcgg aagcggtggc ggaggcgttg gccggcggca
     4501 agcccgcgga gctgacgagt tatcccgaga agctgcgcgc cagttgggtg tgggaggaac
     4561 tgcacggcgt gcgcaacatc cgcccgtcct tcgcgaagtt cggcatgtgg ggcggcctgg
     4621 cctattccgc gctggacacc tacgtgttcc gcggcaaggc gccgtggacc atgcaccacc
     4681 cgcatgccga caacgacacg ctggtcccgg cggagcaggc gccgcgcatc gcctatccca
     4741 agcccgacgg cacgctgacc ttcgaccggc tgtcctcggt gttcatcagc aacaccaatc
     4801 acgaggaaga ccagccggcg catctgcgcc tgcgcgatcc ggacctggcg atcacgacca
     4861 actgggaccg cttccgcagc ccggagacgc gctactgtcc ggccggtgtg tacgagatcg
     4921 tcggtgcgga cgaaggcacg ccgaagctgc agatcaacgc gcagaactgc gtgcattgca
     4981 aaacctgtga catcaaggac ccaggccaga acatcgactg ggtcacgccg gaaggcggtg
     5041 gcggcccgaa ctaccccggc gggatgtaac gcgagggctc cggcacggcg gcgcaggtgg
     5101 tggatcgggc tgaacagtcg tggcgagcga cggtcc
//
LOCUS       DASSZH010000035         2660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_938296, whole genome shotgun sequence.
ACCESSION   DASSZH010000035 DASSZH010000000
VERSION     DASSZH010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938296"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VFN42_00605"
     CDS             complement(<1..263)
                     /locus_tag="VFN42_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995142.1"
                     /translation="MTRLQPVDRLEIQVLVDNVTDGLSSAPAFVTREFAVLQRQGMRI
                     MAGGSLCCANHGLSLVITAHTAGGPRTLLFDGGPVDYAVERNGV"
     gene            complement(329..1171)
                     /locus_tag="VFN42_00610"
     CDS             complement(329..1171)
                     /locus_tag="VFN42_00610"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HET8995143.1"
                     /translation="MTVTPHLRPLGDALGTEVLGINLAQPLDDATFTWIARAFAEHPV
                     LVFRHQELGAGDLASFGRRFGTPRRHALVKYRHAEHPDVSWLTNVDADGKVDWYGVKR
                     ATDWHSDSTYEDTLPILAMLHAKEVPPEKGGTLFADMRSAYDALPEHRKQLLAQLTGL
                     HGRHTGPAGEKLYGTEKGVTEKQYTEVRWPAVVPHPVTGRPILFVNPMHTHGFAGMTR
                     EQAWPLIEELGAHATQDRFVYYHRWQVGDLLMWDERATMHRGAGDSRPDERRVMLRTI
                     VYPN"
     gene            complement(1248..2051)
                     /locus_tag="VFN42_00615"
     CDS             complement(1248..2051)
                     /locus_tag="VFN42_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET8995144.1"
                     /translation="MDVVIAVLNRPEGVQALLEAGARLLEIGGGGRLTALAVRMPPLS
                     TILPSEEVLTAGREAAIRAEQESWAGSLRGIVQQWAPTAQSRGIDTGWVDVEGDAASV
                     VAEHGRRADAIVVSRPASHEAERMRDAVHAALFDTGRPVLVVPPGWNPRFGKTVAIAW
                     KDDARAVKAVHAALPILRHAGRVVVLCARRAAEMPAILAEHGIGAELVSVPDGDAPVG
                     EQLLQAAGAAGADLLVMGAFAHGEWRERVFGGVTRTVLGKADLPLLLRH"
     gene            complement(2080..>2660)
                     /locus_tag="VFN42_00620"
     CDS             complement(2080..>2660)
                     /locus_tag="VFN42_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HET8995145.1"
                     /translation="PDCTGADEAFEVLRSLNFLAGHLEKVRQQPDLVGPNVRANVEEG
                     LRYSAEDVARAQTAQTLLYRRWQTFFQDFDVILTPSITISPRSWRELYPAEIDGKPTR
                     TYFHWLALAYAVTLTGHPAVSLPLGLDRNGMPFGLQIVGPRGGDAFVLSVAAALETML
                     AGDPRTARPVPDLAALRAAPPLRDAPGFIGFD"
BASE COUNT          428 a          967 c          865 g          400 t
ORIGIN      
        1 acgccgttgc gctccaccgc gtaatcgacc gggccgccgt cgaacagcag cgtgcgcgga
       61 ccgcccgccg tgtgcgccgt gatcacaagc gacaggccat gattggcgca gcacagcgag
      121 ccgcccgcca tgatccgcat gccctgccgt tgcagcacgg cgaattcacg ggtgacgaat
      181 gccggagcgg aagacagccc gtcggtgaca ttgtcaacca gaacctggat ctccaggcgg
      241 tccaccggct gcaagcgggt catggcaatt tcctcgaatc tgccggacga cagcgcggca
      301 gcgatcttcc ggcgcccgcc ggacagcgtc aattgggata gacgatggtg cgcagcatga
      361 cccgccgctc gtccggacgg gaatcgccgg ccccgcgatg catggtggcg cgctcgtccc
      421 acatcagcag gtcacccacc tgccagcgat ggtaatagac gaaacgatcc tgcgtggcgt
      481 gtgcgcccag ttcctcgatc agcggccatg cctgttcgcg tgtcatgcca gcgaaaccgt
      541 gcgtgtgcat cgggttcacg aacaggatcg gccgcccggt caccggatgc ggcaccaccg
      601 ccggccagcg cacctcggtg tactgcttct cggttacgcc cttctcggtg ccgtacagct
      661 tctcgccggc cggaccggta tgccgcccgt gcaggccggt cagttgcgcc agcagttgct
      721 tgcgatgctc cggcagcgcg tcgtacgcgc tgcgcatgtc ggcgaacagg gtgccgccct
      781 tctccggcgg cacttccttc gcgtgcagca ttgccaggat cggcagcgtg tcctcatagg
      841 tggaatcgct gtgccaatcg gtggcccgct tcacgccgta ccagtccacc ttgccgtccg
      901 cgtccacatt ggtgagccag gacacgtcgg gatgttcggc atggcggtac ttgaccagag
      961 catgccgccg cggcgtgccg aaccgccggc cgaaggacgc caggtctccg gcgcccagtt
     1021 cctgatgacg gaacaccagc accggatgct ccgcgaaggc ccgcgcaatc cacgtgaagg
     1081 tcgcatcatc cagcggctgg gcgaggttga tgccgagcac ctcggtgccc agagcatcgc
     1141 cgagcggacg caggtgtggc gtgacggtca tgacctatcc tcccgatgca acgccttctt
     1201 atggcggcag catgatcctg ccccggccgt tcggcaacgc tggcgggtca gtggcgcagc
     1261 agcagcggca ggtcggcctt gccgagcacc gtgcgggtga cgccgccgaa cacgcgttcc
     1321 cgccattcgc catgcgcgaa ggcgcccatc accagcaggt cggcgccggc cgcaccggcg
     1381 gcttgcagca actgctcgcc gacgggcgca tcgccatccg gcacgctcac cagttccgcc
     1441 ccgatgccat gctcggccag gatcgccggc atctccgccg cgcgccgcgc gcacagcaca
     1501 accacccggc cggcatgccg gaggatggga agcgccgcat gcaccgcttt caccgcccgc
     1561 gcatcgtcct tccacgcgat cgcgacggtc ttcccgaagc gcgggttcca gcccggcggc
     1621 acgaccagca ccggacgccc ggtgtcgaac aacgcggcat gcaccgcgtc gcgcatgcgc
     1681 tccgcctcgt ggctggccgg acgtgaaacc acgatggcgt cggcccgccg tccatgctct
     1741 gccaccaccg aggcggcatc gccttccacg tccacccagc ccgtgtcgat gccgcgtgac
     1801 tgtgcggttg gcgcccattg ttgcacgata ccgcgcagcg acccggccca gctctcctgc
     1861 tcggcccgga tcgccgcctc ccggcctgcg gtcagcactt cctcggaagg caggatggtg
     1921 gacaacggcg gcatgcgcac ggccagcgcg gtcagccgcc ccccgccgcc gatctccagc
     1981 agacgggcgc cggcctcaag cagtgcctgc accccttccg ggcggttgag cacggcaatc
     2041 acgacgtcca tggcactgtc tcctgtcacg gagcggggat cagtcgaacc cgatgaaccc
     2101 cggcgcgtcc cgcagcggcg gcgcggcgcg caacgctgcc aggtccggga ccgggcgggc
     2161 ggtgcgtgga tcgccggcca gcatcgtctc cagcgctgcc gcgacggaca gcacgaatgc
     2221 gtcaccgccg cggggtccga cgatctgcag cccgaacggc atgccgttgc ggtccagccc
     2281 gagcggcagc gacacggcgg gatggccggt cagcgtcacc gcataagcca gcgccagcca
     2341 gtggaaatac gtccgggtcg gcttgccgtc gatctccgcc ggatacagtt cgcgccagga
     2401 gcgcgggctg atggtgatcg acggtgtcag gatgacgtcg aaatcctgga agaaggtctg
     2461 ccagcggcga tacagcagcg tttgtgccgt ctgcgcgcgg gccacatcct cggcggagta
     2521 gcgcagcccc tcctcgacat tggcgcgcac gtttggccca accaggtccg gctgctggcg
     2581 caccttttcc agatgcccgg ccaggaagtt cagcgaacgc agaacctcga acgcctcatc
     2641 cgcgccagtg cagtcgggcg
//
LOCUS       DASSZH010000036         5143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_733682, whole genome shotgun sequence.
ACCESSION   DASSZH010000036 DASSZH010000000
VERSION     DASSZH010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733682"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..1876
                     /gene="shc"
                     /locus_tag="VFN42_00625"
     CDS             14..1876
                     /gene="shc"
                     /locus_tag="VFN42_00625"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HET8995146.1"
                     /translation="MARRQHADGHWLFELEADATIPAEYVLLEHFLDRIDDALERKIG
                     VYLRAIQGTHGGWPLFHDGAFDLSASVKAYYALKAIGDAPDAPHMARARAAILAAGGA
                     ERTNVFTRAQLALFGQVPWRAVPVMPLEIMHLPLWFPFHLSKVSYWSRTVIVPLLVLM
                     ALQPRARNPRGVQVPELFRTPPEQITDWIRGPYRSSWGRFFKGVDTALRAAQPLFPKR
                     SRDSAIRKAVDFVTTRLNGDDGLGGIYPAIANSVMMFDTLGYPPDHKDAAIAWQAVRK
                     LLVVQEDRAYCQPCLSPIWDTGLAGHAMAEAGVSVDAPCDWLRGRQILDVPGDWTVRR
                     PGLRPGGWAFQYWNDHYPDVDDTAVVGMLLHRQGDPAHAEALDRAREWIIGMQSRGGG
                     KFDGGWGAFEPENIHLHLNHIPFADHGALLDPPTADVTARCVSFLAQLGMSAGDPVMA
                     RALAFLRREQEADGSWFGRWGTNYIYGTWSVLCALNAAGVPPEDAAVRRAVDWLVSVQ
                     RDDGGWGEDEESYAAAPHGRYKESLPSQTAWAVLGLMAGGEAEHPAVARGIAYLSATQ
                     GADGEWTEQPYNAVGFPRVFYLRYHGYRLYFPLLALARYRNLKRGNTRQVAWGF"
     gene            complement(1912..2469)
                     /locus_tag="VFN42_00630"
     CDS             complement(1912..2469)
                     /locus_tag="VFN42_00630"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HET8995147.1"
                     /translation="MLRLTGVTCLGLMLLAAPALAQNGSSGAEGRPQNQKLDQQDQHF
                     LDHAAKDNQGEIRVALLAEKKAESPAVKAFARLMVDDHVAVESQLASVLNRLHSNVSN
                     GVTHEDRQDMQKLEPLHGHAFDRQFMQAQIKDHHGDIQKFHQELQSTHDGSVRAFAVT
                     TLPMLRQHLALAEAVDHSLGGGEKH"
     gene            2993..3637
                     /locus_tag="VFN42_00635"
     CDS             2993..3637
                     /locus_tag="VFN42_00635"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995148.1"
                     /translation="MDATDRITVVVGLQAEARLIHPLGWDVAVGGGSAAGAEAAARSA
                     IAQGASALVSFGLAGGLDPALDPGTLLVPGSVQAAGHRLPTDPALSARLGGVTGHCLL
                     GAAAIVAQADDKRRLFAETSAAAVDLESGAVAQVAAEHALPFAVLRAVCDPAWRSLPP
                     AALWALNARGGIGIGRVVFSVLADFGQLPDLLALARDAAAARRALRRRVAAIRA"
     gene            3696..3977
                     /locus_tag="VFN42_00640"
     CDS             3696..3977
                     /locus_tag="VFN42_00640"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HET8995149.1"
                     /translation="MQAVVNGQQIASSDDIVEAGGYQYFPRSAVRMDWLEKAPKTESD
                     LQCPHGVQFYDVVVDGVRHPRAAWSYEAPQPAMARVAQRMGFWEDVEVR"
     gene            complement(3983..>5143)
                     /locus_tag="VFN42_00645"
     CDS             complement(3983..>5143)
                     /locus_tag="VFN42_00645"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET8995150.1"
                     /translation="VQAGIGGPLAAWLADPRLHRMCLLLAVPLPLVGAAGVMQGVLTT
                     RRRYRTLAWRTLAGQGLGTLAGIGAAGLHAGAWAPVLQQFVVSTIGALALLIGAGVRP
                     HASLHWPVVRGLLRVGLPLTVSTLVLAARYRLFAVLIGGTAGAAALGQVHMAFRLVET
                     VRELAFTAQWRLMLPILSEQRQDRPALRAGCDRLLGLSNLVMLPLCGALALTLPELVR
                     TLLGPGWRAAGWATEPLIALMALLTLTFPSSIALVARGETTRSLIGSLLSTAAVLGGA
                     AVLRPSAPLQATLLWFGAHLLALPYTLRMNGRALGTGPLRPLRAGGPMLAVVLLGVAL
                     GALLPGAVRGGAASLWTGLARLALFAALVVPAVAMQALPASASARVAGLRRR"
BASE COUNT          777 a         1831 c         1800 g          735 t
ORIGIN      
        1 cgcgcgcgac gccttggcgc gccgccagca tgcggacggc cactggttgt tcgaactcga
       61 agccgatgcg acgatcccgg ccgaatacgt gctgctggag cattttctcg accggatcga
      121 tgatgcgctg gagcggaaga tcggggtcta tctgcgcgcg atccagggca cgcatggcgg
      181 ctggccactg ttccatgacg gtgcgttcga tctctcggcc agcgtgaagg cgtattacgc
      241 gctgaaggcg atcggcgacg ctccggacgc gccgcacatg gcgcgggcgc gggcggcgat
      301 cctggcggcg ggcggggccg agcggaccaa cgtgttcacc cgtgcgcaac tggcgttgtt
      361 cggccaggtg ccgtggcgcg ccgtgccggt gatgccgctg gagatcatgc acctgccgct
      421 gtggttcccg ttccacctgt ccaaggtgtc gtactggtcg cgcacggtga tcgtgccgct
      481 gctggtgctg atggcgctgc agccgcgtgc ccgcaatccg cgtggcgtgc aggtgccgga
      541 gctgttccgc accccgcccg agcagatcac cgactggatc cgcgggccgt accgctcgtc
      601 ctggggccgc ttcttcaagg gcgtcgacac cgccctgcgc gccgcccagc cgttgtttcc
      661 gaagcgcagc cgcgacagcg ccatccgcaa ggcggtggac ttcgtcacca cgcggctgaa
      721 cggcgacgac ggcctcggtg gcatctaccc cgcgatcgcc aacagcgtga tgatgttcga
      781 cacgctcggc taccctccgg accacaagga tgcggcgatc gcctggcagg cagtgcgcaa
      841 gctgctggtg gtgcaggagg atcgcgccta ctgccagccc tgcctgtcgc cgatctggga
      901 cacgggcctg gccggccacg ccatggcgga ggccggcgta tcggtggacg ccccctgcga
      961 ctggctgcgc ggccggcaga tcctggacgt gccgggcgac tggacggtgc gccgccccgg
     1021 gctgcgtccc ggcggctggg ccttccagta ttggaacgac cactaccccg acgtggacga
     1081 caccgccgtg gtcggcatgc tgctgcaccg ccagggcgac cccgcgcacg cggaggcact
     1141 ggaccgcgcc cgcgagtgga tcattggcat gcagtcgcgc ggcggcggca aattcgacgg
     1201 cggctggggc gcgttcgaac ccgaaaacat ccacctgcac ctgaaccaca tccccttcgc
     1261 cgaccacggc gcgctgctcg acccgcccac cgccgacgtc accgcgcgct gcgtcagctt
     1321 tctcgcccag ctcggcatga gcgcgggcga tccggtgatg gcgcgcgcct tggccttcct
     1381 gcgccgcgag caagaagccg acggcagctg gttcggccgc tggggcacca actacatcta
     1441 cggcacctgg tcggtgctgt gcgcgctcaa cgccgccggc gtgccgccgg aagacgcggc
     1501 ggtgcggcgc gcggtggact ggctggtatc ggtgcagcgc gacgacggcg gctggggcga
     1561 ggacgaggaa agctacgccg ccgccccgca cggccgctac aaggagagcc tgccgagcca
     1621 gaccgcctgg gcggtgctgg gcctgatggc ggggggggaa gcggagcatc cggcggtggc
     1681 gcgcggcatc gcgtatttgt cggcgacgca gggcgcggac ggggagtgga ccgagcagcc
     1741 gtacaacgcg gtgggtttcc cgcgcgtgtt ctacctgcgc taccacggct accggctgta
     1801 tttcccgctg ctggcgctgg cgcgttaccg caacctgaag cggggcaata cgcggcaggt
     1861 ggcctggggg ttctgagaaa ggcgcccggt tccccgggcg ccatcgccga ctcagtgctt
     1921 ctcgccgcca ccgagtgaat ggtcgaccgc ctcggccagg gcaaggtgct ggcgcagcat
     1981 cggcagcgtg gtgaccgcga aggcccgcac cgacccatca tgagtggact gaagttcctg
     2041 atggaacttc tggatatcgc cgtggtggtc cttgatctgc gcctgcatga actggcgatc
     2101 gaatgcatgg ccatgcaacg gctccagctt ctgcatgtcc tgccgatcct cgtgcgttac
     2161 gccgttcgac acattgctgt gcaggcgatt caacacgctg gcgagttgcg attcgacggc
     2221 aacatggtca tccaccatca agcgggcaaa cgccttcacc gcggggcttt ccgccttctt
     2281 ttccgcgagc agcgcaaccc ggatctcccc ttggttgtcc ttggcggcat gatccaggaa
     2341 atgctggtcc tgctggtcca gcttctggtt ctgcggccgg ccttcggcgc cggatgaccc
     2401 gttctgcgcg agggccggtg cggcgagcag catcaacccg aggcaggtaa cgccggtcag
     2461 tcgaagcatc atcgttctcc agttctgtgg aggtgaaagg cgtcaaccgc tgcccgcgct
     2521 gcatcacgaa cgcgcggcgc catgccgggc gtctcctgga ttcggggtgg tcaatgtcgg
     2581 tccgcacgaa ggcggtcgcc gatcgcccag ctccgccgcc acggttccgc attcaatgcc
     2641 ggatcagatg agtcccggct gctcggcgtg ctttgccgcg gcgcagaaac gcaaacatcc
     2701 gaatgggcaa tttttcgaag cgacaagcac aagatcgcgc atctggcgca tgccggcgcg
     2761 gctcctatgg aattggcgtc agtcttcgca gcttccgcca gccgcggcga tcggtgccca
     2821 ggcatccagg gcggcgaacc ggttatcgtg cgacaattaa gcgcctagcg gcttgagctt
     2881 ccatcccgcc cgccggccac acggccggca cgcgtgccct tcggatgcta caggcgaatt
     2941 ggcggttccg ccagagtggg ttaggtcggg cgtgcagcac ggagcagggc gcttggacgc
     3001 aacggaccgg atcaccgtcg tggtcggatt gcaggccgaa gcacgcctga tccacccgct
     3061 cggctgggac gtcgcggtcg gcggcggttc cgccgcgggg gcggaagcgg cggcgcgctc
     3121 ggccatcgcc caaggcgcgt ccgcgctggt cagcttcggt ctggccggcg ggctggaccc
     3181 ggcgctggac ccggggacgc tgctggttcc cggtagcgtg caggccgccg gccaccgtct
     3241 gcctaccgat cccgccctgt ccgcccggct tggcggcgtg accgggcatt gcctgctggg
     3301 cgccgccgcc atcgtcgccc aggccgacga caagcgccgg ctgtttgccg aaaccagcgc
     3361 ggctgcggtc gacctggaaa gcggcgccgt cgcgcaggtg gcggcggagc acgcgctgcc
     3421 cttcgcggtg ctgcgcgcgg tctgcgatcc ggcttggcgc agcttgcctc ccgccgcgct
     3481 ctgggcgctg aacgcgcgag gcggcatcgg catcggccgc gtggttttct ccgtgctggc
     3541 cgatttcggg cagctccccg acctgctggc gctggcccgc gacgccgccg ccgcccggcg
     3601 cgccttgcgg cggcgggtgg cggcgatccg ggcctgaccc attgctccag gccgtgccac
     3661 gcagtaccca tcatgcaccg gacagggagg cgagcatgca agcagtggtg aacggccagc
     3721 agatcgccag cagcgacgac atcgtcgagg ccggcggcta ccagtatttc ccccgcagcg
     3781 cggtgcgcat ggactggctg gagaaggcgc cgaagaccga gtcggacctg caatgcccgc
     3841 acggcgtgca gttctacgac gtcgtggtgg acggtgtgcg gcatccacgc gccgcctggt
     3901 cctatgaggc gccgcagccg gcgatggccc gcgtcgccca gcgcatgggc ttctgggaag
     3961 acgtcgaggt ccgctgaccg gttcagcgcc ggcgcaatcc ggcgacccgt gccgatgccg
     4021 aagccggcag cgcttgcatg gcgacggccg gcacgaccag cgcggcgaac agcgccagcc
     4081 gagccaagcc ggtccacagc gaagccgcgc cgccgcgtac cgcacccggc agcagcgcac
     4141 ccagcgccac gcccagcagc acgaccgcta gcatcggccc accggcgcgc agcgggcgca
     4201 gcggaccggt gcccaaggcc ctgccgttca tccgcaacgt atagggcagc gccagcaggt
     4261 gcgcgccgaa ccacagcagc gtcgcctgca gcggcgccga tggccgcaac accgccgcgc
     4321 cgcccagcac cgcggcggtg gagagcaggc tgccgatcag cgagcgtgtg gtctcgccgc
     4381 gcgccaccag ggcgatgctg gacgggaaag tcagcgtcag cagcgccatc agggcgatca
     4441 gcggttcggt ggcccagccg gcggcgcgcc agcccggacc cagcagcgtc cggaccagct
     4501 ccggcaacgt cagcgccaga gcgccgcaca gcggcagcat caccaggttg gacaggccca
     4561 gcagccggtc acatcccgcc cgcaaggccg gccggtcttg ccgctgctcc gacaggatcg
     4621 gcagcatcag ccgccattgc gcggtgaagg ccagctcccg cacggtctcc accagccgga
     4681 atgccatgtg cacctgcccg agcgccgccg cgccggccgt gccgccgatc agcacggcga
     4741 acagccggta gcgcgccgcc agcaccagcg tgctgacggt cagcggcagc cccacccgca
     4801 gcaggccgcg caccacgggc caatgcaacg aagcatgcgg ccgcacgccg gcgccgatca
     4861 gcaatgccag ggcgccaatg gtggagacga cgaactgctg cagcaccggt gcccaggccc
     4921 cggcatgcag gccggccgcg ccgatgccag cgagcgtgcc cagcccctgc cccgccagcg
     4981 tgcgccaggc cagggtccga taacggcgcc gggtggtcag cacgccctgc atcaccccgg
     5041 ccgccccgac cagcggcaac ggcacggcca gcagcaagca catgcgatgc aggcgcggat
     5101 cggcgagcca cgccgccagc ggcccgccga tcccggcctg aac
//
LOCUS       DASSZH010000037        13283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1330191, whole genome shotgun sequence.
ACCESSION   DASSZH010000037 DASSZH010000000
VERSION     DASSZH010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 13283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13283
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330191"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1644)
                     /locus_tag="VFN42_00650"
     CDS             complement(<1..1644)
                     /locus_tag="VFN42_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8995151.1"
                     /translation="MHFDRQLWAMTAGQRGRIALSTLLGLLALAFGIARFAFLGRFLA
                     GVFQGAPAPALALPLAAAGVAIVLRCVLEHARTMVAHRTAARVQEQLRARLFDKIVAL
                     GPAWFGAERTGGVMLSMVDGVEQLQSFFGQYLPQVAVAIAAPVAIFAFIAWWDVPVAS
                     VMLVAALATLVLPAVVHERSGEASRERQRAFKAFGEELLDAIQGLPTLKAFGQSSRYG
                     EMLAEKARALSDSTFWVLSWSVLTRGITDTGCALGAAGAMALGAWRVHAGDMSLEALL
                     IVLMAGTEIFRPLRDLRTVLHQGLTGQAAADGILKLLAAPASAPAAPRTPIAASNLRP
                     RIEFQDVRFAYPGGRAPAHQGLGFAIEPGERVAVVGASGSGKSSIVKLLLRLYDPQSG
                     SIRIDGQDLRALDPAVVRGMIAVVAQDSMLFHGTIAENLRLGRPNATEQEVVAAAIAA
                     NAHEFIMALPDDYDTVIGERGLQLSGGQRQRLAIARALLRDAPILVLDEALSSVDAEN
                     EALIQQALDRLMQGRTTLILAHRLSSVIGADRILVLADGA"
     gene            1840..3513
                     /locus_tag="VFN42_00655"
     CDS             1840..3513
                     /locus_tag="VFN42_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET8995152.1"
                     /translation="MAPSASYHWPASSPERLRLERDRHVAFAFAAADLLIETAIDGAI
                     VAASGAAQAVLGVAVRALPGRALAELVVPGDRGLVRRLLQQVHQVGRIDPVTTHLIRP
                     DGVTSRVLLGACCLPNGGDQVFLSASLLPTTLTAAVQARDAATGLLSPEALREAAQRG
                     CTEDGGGKRELKLLHLQGLSGAVRQLPADRSAMLLQEIGAALRAVSVGGDAAGRLGED
                     TFGLVARSGHRDAALVAELRDVFRDAGLAEGQIGPRLARVDLALGGLSGGEAGQALSY
                     AMNSFVAAQGSAFNIGSLREGLAAAVDATVNRFAETRQIISELRFSLVFQPVVALAGR
                     AVHHYEVLSRFHGKPNTFEMVGFMEDVGLVSELDLAVCRMTLAALEQNPGVRLAINLS
                     GRSVQSEAFRNQLSALISGVALLGDRLMFELTESAAVERLEEAASFLGWLRQRGHQVC
                     LDDFGSGAAAYSYLRRFDVDYLKIDGPFLKAAQEMGRERALIRSICVLCDEIGAKVIG
                     EMIEDEKAAALAKSLGIGYGQGWLFGKPMRELPKPVTASRRKGAVETWE"
     gene            complement(3543..4700)
                     /locus_tag="VFN42_00660"
     CDS             complement(3543..4700)
                     /locus_tag="VFN42_00660"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014104838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HET8995153.1"
                     /translation="MDQSAILTALDHTHAVFEGPFALDCGVSLPRITVAYRTYGTLNA
                     ARSNAVLVCHALTGDQYVAERHPVTGKDGWWDAVVGPGRPVDTDRFFVICANVLGGCM
                     GSTGPRSPRDSAPGMVWGTDFPPVTIRDMVRAQKRLIDHLGIDRLFAVLGGSMGGMQV
                     LEWAASYPDAVFAALPIATAAYHSAQNIAFHEVGRQAIFADPDWEGGRYWTTGKIPAR
                     GLAVARMCAHITYLSEQALTRKFGRRLQNAPKDPSEAISLFGGMFEVESYLHHQGSTF
                     VQRFDANSYLTITRAMDYFDLAADYQGDLANAFRGTRTRFLLVSFSSDWLFPTPESRS
                     IARALNRAGANVSFVEIPSDKGHDAFLLDEPDFHRTLAGFLDGCAVHAGLS"
     gene            complement(4766..5017)
                     /locus_tag="VFN42_00665"
     CDS             complement(4766..5017)
                     /locus_tag="VFN42_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HET8995154.1"
                     /translation="MDVQASMLDRLTTRVVLPLVPQTQAPPPIRELNPIFEIDGTPHV
                     LLTQAIAAVPAKELKRAVASLDGQHDQIIRALDVLLLGF"
     gene            complement(5175..5348)
                     /locus_tag="VFN42_00670"
     CDS             complement(5175..5348)
                     /locus_tag="VFN42_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995155.1"
                     /translation="MLKPAELHRRAADLRKRAEDAPDPLWKVTLNTIAEEFEHDAVEE
                     ERLAPGAHSPPRA"
     gene            complement(5476..7002)
                     /locus_tag="VFN42_00675"
     CDS             complement(5476..7002)
                     /locus_tag="VFN42_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HET8995156.1"
                     /translation="MVNWTALIIFVLLFAFITWIGFIAAHWRKGDLDLLHEWGLGGRR
                     FGTWVTWFLIGGDLYTAYTFIAVPALLFGAGAIGFFAVPYTIMVYPILYLVFPRLWSV
                     AHRRGYITSADFVRGRFDNRWLALAVAFTGILATMPYIALQLVGMQVVIGAMGVQTGG
                     LTGDLPLIIAFVILAAFTYTSGLRAPAMIAVVKDVLIYLTVLAAIIVVPIHLGGYDKI
                     FTAIPAPKLLLAHPPAGSYGSFSAYATLALGSALALFLYPHSITGIFGASSARVVRRN
                     AALLPTYSLMLGLLALVGFMAIAVGVQKMPEFAGGFKAYSNNFSVPALFLAVFPSWFV
                     GIAFAAIAIGALVPAAIMSIATANTFTRNIYREFLHPGCTDAEESQVAKIVSLIVKAG
                     ALAFIFGLPLQFALYLQLLGGIWIIQTLPSVILGLYARMLNGWALLIGWIVGFVLGTW
                     LVVQGNFVPIYPLHVFGTVFPCYIALIAVAANIVVSFVLSIPLNAMAPGRGDVAVADF
                     "
     gene            complement(7005..7217)
                     /locus_tag="VFN42_00680"
     CDS             complement(7005..7217)
                     /locus_tag="VFN42_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HET8995157.1"
                     /translation="MQKPQPAEPAPRSVLWARLLLIVPFVAMLWVSSYDRVAPSIAGI
                     PFFYWYQLAWVIIGAVIIGVVYVVEH"
     gene            complement(7376..8083)
                     /locus_tag="VFN42_00685"
     CDS             complement(7376..8083)
                     /locus_tag="VFN42_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8995158.1"
                     /translation="MTPLLSVEHIDAAYGLSQVLFGISLEVMQGECVCLLGRNGVGKS
                     TTMRSVMGLTPPRSGRVVWRGQDVTGWPPYRIARAGIGFVPEDRRIFAELSVWENLDV
                     AARAARRSGRWSIEQVCELFPVLAERRNQRGGFLSGGEQQMLTIGRALVGNPELLLLD
                     EPSEGLAPLVVELLRQKIAELKSEGLTILLAEQGVAFSLSLADRVYVLEKGTVRHAGP
                     AAELRGDTALLDRLLAL"
     gene            complement(8136..8873)
                     /locus_tag="VFN42_00690"
     CDS             complement(8136..8873)
                     /locus_tag="VFN42_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8995159.1"
                     /translation="MLEISHLRKSFGGFVAVSDVGFTVETRQIAAVIGPNGAGKSTLF
                     NLITGHLRSDAGRVVLDGQDVTGLPPHRICALGMGRSFQLTNIFPRLTVLENVQAALL
                     AHRGHGWNFWSRSEMLYREDAMLLLASVGLAGVASEIAGTLAYGNQKQLELGIALASD
                     PRVLLLDEPTAGMSARETHETIDLLRRIAVERELTLLFTEHDMSVVFGFAQKIAVMYQ
                     GRKLAEGTPDEVRGNPEVRRVYLGEHA"
     gene            complement(8866..9885)
                     /locus_tag="VFN42_00695"
     CDS             complement(8866..9885)
                     /locus_tag="VFN42_00695"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8995160.1"
                     /translation="MSVPLSRPLVHPARPLIRRVPWTLLIFLAMLPLPWIGSRYGTFL
                     ATQIAIDALFAVSLNLLLGTTGLVSFGHVAYFGIGAYVCGILMKTCGWPFAIAFPAAG
                     LGAAAFSLIFGYFCVRLTKIYFAMLTLAFSQIVWAVCFKWDAVTGGDQGIADIPFPDM
                     HWMAAIPGLDALPENSRFYLLTLTLVAVSIAAIGQITRSPFGRMLTAIRDNRERAAFI
                     GLHVRAYELAVFVVAGGFAGLAGALYGIFTRGVFPDYVFWPKSAEVMIMAILGGMNFF
                     WGPAVGALVLEWLNQEITNYTQYWPFVLGSILLVLLFVLPGGVLGGLARLGGVLQRRM
                     GRRDA"
     gene            complement(9882..10763)
                     /locus_tag="VFN42_00700"
     CDS             complement(9882..10763)
                     /locus_tag="VFN42_00700"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8995161.1"
                     /translation="MTLSLISAQFFSGLTTAMFLFLIASGLSLVFGIMRVLNFAHGSF
                     YMIGAYMAWQVVTWLQPTGGAFWIAALAAGVSVAILGGVVERLLLRHLYGVDELYQLL
                     FTYALVLILGDAAKFTWGTDQLSVHRPPVLDGSFHLLGTTLPFYDLFIILVGPAIAIA
                     GGLILSKTSAGRLLRAAAYDREMLGALGANVGALYTGMFVVSSFLAGLAGALVTPIES
                     VVPGMDVQIIVNAFIVVVIGGLGSFWGTFWGAMIYGQTLSFGILIFPQFSVFAVFALM
                     AVVLVVRPWGLLGRPIR"
     gene            complement(11127..12326)
                     /locus_tag="VFN42_00705"
     CDS             complement(11127..12326)
                     /locus_tag="VFN42_00705"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8995162.1"
                     /translation="MEIRHALAAMAFALAMPAATYAKEPIKIGFPIPLSGPTAVYGVP
                     ILKGAELAVADINAAGGVLGRKLELLPRDSKANPAEAVRLARELIVRNGVQFLAGTLT
                     SAEAPAVSTIAKENHVVFDTPSAKTSALTDAAHIHPYVFRSASNTTMEGQAGAIIMAR
                     WKDVKTVATVAPDYAYGHDAVAAFVAKLKQLRPDITIVDQQWPKLGESNFTPFITAQM
                     SKHPDAVYCDEFAGDFISFVKQGAPLGYFKAVHNRVITGGEVGSVDVTQSLGSSFPFG
                     IWANTYDPVVWHGPNQPAAEKVFDAKVAKAMGTKYASSWAIQGYVVIEAIAQGIAKAG
                     TTDDAKVAAVMAGMTYQTPLGPRTFNSKTHDADAGEYWGEMTQDPSVPFAVIKNPVYY
                     NPEPAMH"
     gene            complement(12458..>13283)
                     /locus_tag="VFN42_00710"
     CDS             complement(12458..>13283)
                     /locus_tag="VFN42_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HET8995163.1"
                     /translation="SNATAPCGLAWRWRTLLPESGLLPRLAAGASAAGRATLDLLLPP
                     QCICCDAAVSRPGQFCAACFRATAFITAPYCLRCGVPFGNAGQGGLRQLCPVCETDPP
                     RFGQARAALRYDKQGRRLILPFKHADRPDLAAILAPMMARAGAALLNRAELLVPVPLH
                     RLRLFRRRYNQAALLAQALARLSRVPASVDALQRRRATDSLGEKSAAERAETVRDAFA
                     VRPSRRPVVAERRVLLIDDVMTSGATANACAETLLAAGASAVDVLVAARVPDPRLS"
BASE COUNT         2399 a         4399 c         4496 g         1989 t
ORIGIN      
        1 cgcaccatcc gccagcacca ggatgcggtc agcgccgatt acgctggaca gccggtgtgc
       61 caggatcagg gtggtgcggc cttgcatcag ccgatccagc gcctgctgga tcagcgcctc
      121 gttctcggcg tccaccgacg aaagtgcctc atccagcacg agaatggggg catcccgcag
      181 cagggcacgg gcgatcgcca gacgctggcg ctggccgccg gacaactgca gcccgcgctc
      241 gccgatcacg gtgtcgtagt cgtcgggcag cgccatgatg aactcatgcg cgttggcggc
      301 aatggcagcg gcaaccacct cctgctcggt cgcgttgggg cggccaagcc gcaggttttc
      361 cgcgatcgtg ccgtggaaca gcatgctgtc ctgcgccacc acggcgatca tcccgcgcac
      421 caccgccggg tccaatgccc gcagatcctg gccgtcgatg cggatgctgc cggattgcgg
      481 gtcgtacagc cgcagcagca atttcacgat cgacgacttg cccgagccgc ttgcgccgac
      541 cacggcgacg cgctcgcccg gctcgatggc gaaacccagg ccctggtgtg caggggctcg
      601 gccgcccggg taggcgaagc gcacatcctg gaattcgatg cgcggacgca gattggacgc
      661 cgcgatcggc gtgcgtggtg ccgcgggcgc ggatgccggc gcggcgagca gtttcaggat
      721 gccgtcggcg gcggcctgcc cggtcaggcc ctggtgcagg accgtgcgca ggtcgcgcag
      781 cgggcggaag atttccgtcc ccgccatcag cacgatcagc agcgcctcca ggctcatgtc
      841 gccggcatgc acgcgccagg cccccaatgc catggcgccg gcggcaccca gggcgcagcc
      901 ggtatcggtg atgccacgtg tcagcacgct ccacgacagc acccagaacg tgctgtccga
      961 caaagcgcgc gccttctcgg ccagcatctc gccgtagcgg ctgctttggc cgaaggcttt
     1021 cagcgtgggc aggccctgga tggcgtccag caactcctcg ccaaatgcct tgaaggcgcg
     1081 ctgccgctcg cgtgacgcct cgccggagcg ttcatgcacc acggccggca acaccagcgt
     1141 ggccagagcg gcgaccagca tcacggatgc gacgggcaca tcccaccagg cgatgaaggc
     1201 aaagatcgct accggggcgg cgatggcgac agcgacctgc ggcaggtact ggccgaagaa
     1261 gctctgtagc tgctcgacgc cgtccaccat cgacagcatc acgccgccgg tgcgttccgc
     1321 cccgaaccag gccgggccga gcgcgacaat cttgtcgaac agccgggcgc gaagctgctc
     1381 ctgcacccgc gcggcggtac ggtgcgccac catggtccgg gcgtgttcca gcacgcatcg
     1441 tagcacgatg gccacgccgg ccgcagccag cggcaaggcc agtgccggtg ccggcgcacc
     1501 ctgaaacact ccggccagga agcgcccgag aaacgcgaag cgggcgatgc cgaaggccag
     1561 ggccaggagg ccaagcaggg tggacagcgc gatgcggccg cgctggccag cagtcatcgc
     1621 ccagagttgc cggtcgaaat gcatggcgct caatgtgggg cgggagaagg cggcgcctca
     1681 agcccggtgc gggcgcgaga agctgcggct gcgggatcgg tcatccggca cctgacatta
     1741 acgactcctc aatcaccccc ccgctagaag gctttatccg gcccgcggtc gtgttcgcct
     1801 gctccgcctg cccggtctga ccaccaggat caacacggcg tggcaccttc ggcgagctac
     1861 cactggccgg ccagttcccc cgagcgcctg cggctcgagc gcgaccgcca cgtggcgttt
     1921 gccttcgccg cggccgatct gctgatcgaa accgcgatcg atggcgccat tgtggccgcc
     1981 tccggcgcgg cacaggcggt gctgggcgtg gccgtccgtg cgctgccggg ccgggccctg
     2041 gcggagctgg tggtgccggg cgatcggggg ctggtgcggc gcctgctgca gcaggtgcac
     2101 caagtcgggc gcatcgatcc ggtcaccacc cacctgatcc gaccggacgg agttaccagt
     2161 cgcgtgctgt tgggcgcctg ctgcctgccc aatggcggcg atcaggtgtt cctcagtgcc
     2221 agcctgttgc cgaccacgct gaccgccgcc gtgcaggcac gcgacgcggc caccggcctg
     2281 ctcagtcccg aggccttgcg ggaagcggcc caacgcggct gcaccgagga tggcgggggc
     2341 aagcgcgaac tgaaactgtt gcatctgcag ggattatccg gcgccgtacg ccagttgccg
     2401 gctgaccgca gtgccatgct gctgcaggag atcggcgccg cgctccgcgc cgtgtcggtc
     2461 ggcggcgatg cggcggggcg gctgggggaa gacacattcg ggctggtggc ccggtccggc
     2521 caccgcgatg ccgcactggt agcggagctg cgcgacgtgt tccgtgatgc cggcctggcc
     2581 gaagggcaga tcgggccgcg tctggcgcgg gtcgatctgg cgctgggtgg gctgagcggc
     2641 ggcgaggccg gccaggcgct aagctatgcc atgaacagct tcgtcgcggc gcagggcagc
     2701 gcgttcaata tcggttcgct gcgggagggc ttggcggcgg cggtggacgc gacggtgaac
     2761 cgctttgccg aaacgcggca gatcatcagc gagctgcgct tctcgctggt gtttcagccc
     2821 gtggtcgcgc tggccggccg ggcggtgcac cactacgagg tgctgtcccg ctttcacggc
     2881 aagccgaaca cgttcgaaat ggtcggcttc atggaggatg tcggcttggt gagcgagctg
     2941 gatctggcgg tctgccggat gacgctggcc gctctggagc agaacccggg tgtccggctg
     3001 gccatcaacc tgtccggccg ctcggtgcag agcgaagcct tccgcaacca gttgagcgcc
     3061 ctgatcagcg gcgtggccct gctgggcgat cgtctgatgt tcgaactgac cgaatccgcg
     3121 gccgtggagc ggctggagga ggccgcgtcc ttcctgggct ggctgcggca gcgggggcat
     3181 caggtgtgcc tggacgattt cggctccggg gcagcggcgt acagctacct gcggcggttc
     3241 gatgtcgact acctgaagat cgacggtccg ttcctgaagg cggcgcagga aatggggcgg
     3301 gagcgcgcgc tgatccgctc gatctgcgtc ctgtgcgacg agatcggggc gaaggtgatc
     3361 ggcgagatga tcgaggacga gaaggcggcc gccttggcga aatcgctcgg gatcggctac
     3421 ggccagggct ggctgttcgg caagccgatg cgcgagctgc cgaagccggt cacggcatcg
     3481 cggcggaagg gcgccgtgga gacctgggag tagcggccgc tggtccggac ggcgccggga
     3541 gcctagctca gtccggcatg caccgcgcaa ccgtcgagga atccggccag ggtgcgatgg
     3601 aaatccggct cgtccagcag gaaggcgtcg tgtcccttgt cgctggggat ttccacgaag
     3661 gacacgttgg cgcccgcccg gttcagcgcg cgcgcgatcg accggctttc cggcgtcggg
     3721 aacagccagt cggagctgaa cgacaccagc aggaagcggg tcctggtgcc gcggaacgcg
     3781 ttggccaggt cgccctggta gtccgcagcc aggtcgaaat aatccatcgc ccgggtgatg
     3841 gtcaggtagg aattggcgtc aaagcgctgc acgaaggtgc tgccctggtg gtgcaggtag
     3901 gattccacct cgaacatgcc gccgaacagg ctgatcgcct cgctggggtc cttcggcgcg
     3961 ttctgcagtc ggcggccgaa tttccgtgtc agcgcctgct ccgacagata ggtgatgtgc
     4021 gcgcacatcc gcgccaccgc caggccgcgt gccggaatct tgccggtggt ccagtagcgt
     4081 ccgccttccc agtccggatc ggcgaagatc gcctggcgcc cgacttcgtg gaaggcgatg
     4141 ttctgcgccg agtggtaagc ggccgtggcg atcggcagcg ccgcgaacac cgcgtcgggg
     4201 taggacgccg cccattccag tacctgcatc ccgcccatcg agccgccgag cacggcgaac
     4261 aggcggtcga tgcccaggtg gtcgatcagc cgcttttgtg cccgcaccat gtcgcggatg
     4321 gtgactggcg ggaaatcggt gccccagacc atgccgggtg cgctgtcgcg cggcgagcgc
     4381 ggcccggtgg agcccatgca gccgcccagc acgttggcgc aaatgacgaa gaagcggtcg
     4441 gtatcgaccg ggcggccggg gcccacgacg gcgtcccacc agccatcctt gccggtgacc
     4501 gggtgccgct ccgccacgta ctggtcgccg gtgagggcgt gacagaccag caccgcgttg
     4561 ctgcgggcgg cattcagtgt gccgtaagtg cgataggcca cagtgatgcg cggcagcgag
     4621 acgccgcagt cgagggcgaa gggcccctcg aacaccgcgt gggtgtgatc cagcgcggtc
     4681 aggatggcgg actggtccat ggcccggatg catagggcgc gcgcgggcgg cgggcaacgg
     4741 ggtgggcggt cacgggcgga attgctcaaa aacccagcag caagacatca agggcccgga
     4801 taatctggtc gtgctgaccg tcgagcgatg cgacggctcg tttcagctcc ttcgccggaa
     4861 ccgcggcgat cgcctgtgtc agcaggacgt ggggcgttcc gtcgatctca aatatcgggt
     4921 tgagctcgcg gatcggcggc ggcgcctggg tttgcggcac caatggcagc accacgcgcg
     4981 tcgtcagtcg atcgagcata ctggcctgca catccaccct gcacatccac aatgtacccg
     5041 gcacccgctg cgccgggcat gcgatagacg tcgagacgcg ccatcagaac tggcggaact
     5101 gggcgagtgg caatccgggg ccgagtccgc ccgcaccggc cctcgccgcc ggcgttcgtt
     5161 ctgaaggctg actgctacgc gcggggcggg ctgtgtgccc ctggagcaag ccgctcctcc
     5221 tccactgcgt cgtgttcgaa ctcctcggcg atcgtgttca aggtcacctt ccacagcggg
     5281 tccggtgcgt cctcggccct tttgcgaagg tcggcggccc ggcggtgcag ttcggcaggc
     5341 ttgagcatgg cgatcccctg tgcgctcgta caaacccatg cgatggtccg tgcggcaacg
     5401 ccggggtccg ggtgcgtttt cgcacccggg ttgtcaaacc gccgtcacag gcggggcggc
     5461 gggccccgcc cgagctcaga agtcggccac cgccacgtcg ccccggccgg gtgccatcgc
     5521 gttcagggga atcgaaagca cgaaggacac cacgatattc gccgccacgg cgatcagcgc
     5581 gatgtagcag ggaaacaccg tgccgaagac gtgcagcgga tagatcggca cgaaattgcc
     5641 ttgcaccacc agccaggtcc ccagcacgaa gccgacgatc cagccgatca gcagcgccca
     5701 cccgttcagc atgcgcgcat acaggcccag gatcaccgag ggcagcgtct ggatgatcca
     5761 gatgccaccg agcaactgca ggtacagcgc gaactgcagc ggcaggccga agatgaaggc
     5821 cagcgcaccg gccttcacaa tcaacgacac gatcttcgcc acctggcttt cctcggcatc
     5881 ggtgcagccg gggtgcagga actcgcgata gatgttgcgg gtgaaggtgt ttgccgtggc
     5941 gatcgacatg atggccgccg gcaccagcgc gccgatcgcg atcgccgcga aggcgatgcc
     6001 gacgaaccag ctcgggaaca ccgccaggaa cagcgccggg accgagaagt tgttggagta
     6061 ggctttgaac ccgccggcga actccggcat tttctgcacg ccgacggcga tggccatgaa
     6121 gccgaccagc gccagcagcc ccagcatcag cgaataggtc ggcagcagcg ccgcgttgcg
     6181 ccgcaccacg cgggcgctgg aggcgccgaa gatgccggtg atcgagtgcg gatacagaaa
     6241 cagcgccagc gccgagccca gcgccagcgt ggcataggcg ctgaagctgc cataggagcc
     6301 ggccggcgga tgcgccagca gcagcttggg tgccgggatc gccgtgaaga tcttgtcgta
     6361 gccgcccagg tggatcggca cgacgatgat ggccgccagc accgtcagat agatcagcac
     6421 gtccttcacc acggcgatca tcgccggggc gcgcaggccg gaggtgtagg tgaacgcggc
     6481 caggatgacg aaggcgatga tcaacggcag gtcgccggtg aggccgccgg tctgcacgcc
     6541 catcgcgccg atcaccacct gcatgccgac aagctgcaac gcgatatacg gcatggtcgc
     6601 gaggatgccg gtgaaggcca cggccagcgc gagccagcgg ttgtcgaacc ggccgcgcac
     6661 gaaatcggcc gaggtgatgt agccacgccg atgcgccacc gaccacaggc gcgggaaaac
     6721 cagatagagg atcgggtaca ccatgatggt gtacggcacg gcgaagaagc cgatcgcccc
     6781 ggcgccgaac agcagggcgg gaaccgcgat aaaggtatag gcggtataga ggtcgccgcc
     6841 gatcaggaac caggtcaccc aggtgccgaa gcgccggccg ccgaggcccc attcgtgcag
     6901 caggtccaaa tcgcccttgc gccaatgcgc ggcgatgaag ccgatccagg tgatgaaggc
     6961 gaacagcagg acgaagatga tcagcgcggt ccagttcacc atggtcagtg ttccaccacg
     7021 taaaccacgc cgatgatcac cgccccgatg atcacccagg caagctggta ccagtagaag
     7081 aacggaatcc cagcgatgct gggtgcaacc cggtcgtagg aggagaccca cagcatcgcc
     7141 acgaagggta cgatcagcag caggcgcgcc cagagcacgc tgcgcggtgc aggctcggcc
     7201 ggttgaggtt tttgcatggt gcgcttcacc tggaactcga ggggcttgcg tcccgacaag
     7261 gccgggctcg cttttggcgc tctttggcac aatgcttgat cgctatcaat ccattggcgt
     7321 gcggctttac cccgcggcgc gggcaagcct tggcctggac ttcaagccgc cggcactaca
     7381 gcgccagcag ccggtccagc agcgcagtgt cgccgcgcag ttcagccgcg gggccggcgt
     7441 ggcggaccgt gcccttttcc agcacataga cccgatccgc cagcgacagc gaaaacgcca
     7501 ccccctgctc cgccagcagg atggtcaatc cttccgactt cagttcggcg atcttttgcc
     7561 gcagcagttc cacgaccagg ggggccagtc cctctgaagg ctcatccagc agcagcagtt
     7621 ccggattgcc gaccagcgcc cgtccgatcg tcagcatctg ctgttcgccg cccgacagga
     7681 acccgccccg ctggttgcgc cgttccgcca gcacgggaaa cagctcgcag acctgctcga
     7741 tcgaccagcg gcccgagcgc cgtgccgcac gtgcggcgac atccaaattc tcccagacgc
     7801 tcaactcggc gaaaatccgc cggtcctccg gcacaaagcc gatgccggcc cgggcgatgc
     7861 gatacggtgg ccagccggtc acgtcctgtc cgcgccatac cacgcggccg gagcgcggcg
     7921 gagtcagccc catgacggag cgcatggtgg tggattttcc gacaccgttc cgcccgagca
     7981 ggcacacgca ttcaccctgc atcacctcga gtgagatgcc gaacaggacc tggctcaggc
     8041 cgtaggcagc gtcgatgtgt tcgacggaca gcaggggcgt catcgccgca gtactccggc
     8101 gtgccgggaa cgtgcatgaa gccagcgatg caggatcacg cgtgctctcc cagatagacg
     8161 cggcgcacct cgggattgcc tcgcacctcg tccggtgtgc cttccgccaa tttgcgaccc
     8221 tgatacatca cggcgatctt ctgcgcgaac ccgaacacca ccgacatgtc gtgctcggtg
     8281 aacagcagcg tcagttcgcg ttcaactgca atgcgccgca acaggtcgat tgtttcatgg
     8341 gtttcccgcg cggacattcc cgcggtgggc tcgtcgagca gcagcaccct gggatcggag
     8401 gccagggcaa tgccaagctc aagttgcttc tggttgccgt aggccagcgt gccggcgatt
     8461 tcggatgcaa caccggcgag cccgacggag gcgagcaaca gcatggcatc ttcgcgatac
     8521 agcatctccg accgcgacca gaagttccag ccgtgcccgc gatgcgccag caacgccgcc
     8581 tgcacgttct ccaggaccgt cagacgcgga aagatgttgg tcaattggaa ggaccggccc
     8641 atgccgaggg cgcagatgcg atgtggcggc aggccggtca cgtcctgccc gtcgagcacg
     8701 acgcgacccg catcgctgcg cagatgcccg gtaatcaggt tgaacagcgt cgatttgcct
     8761 gccccgttcg gtccgatcac cgcggcgatc tggcgggttt ccacagtgaa tccgacgtcg
     8821 ctgaccgcga caaacccgcc gaacgatttc cgcagatggc tgatctcaag catcgcggcg
     8881 acccatccgg cgctgaagca caccgcccag ccgcgccagg ccgcccagca cgccgccggg
     8941 cagcacgaac agcagcacca gcagaatgct gcccagcacg aagggccagt attgcgtgta
     9001 gttggtgatt tcctggttca gccattccag caccagtgcg ccgacagcag ggccccaaaa
     9061 gaagttcatg ccccccagga tggccatgat catcacctcg gcggatttgg gccagaacac
     9121 gtaatcgggg aagacgccgc gggtgaagat gccgtacagg gcaccggcca ggccggcaaa
     9181 accgcctgcc accacgaaga ccgccagttc atacgcccgc acatgcaggc cgatgaaggc
     9241 ggcgcgttcg cgattgtcgc ggatcgccgt cagcatgcgg ccaaacggcg atcgggtgat
     9301 ctggccgatg gcggcaatcg aaaccgccac cagggtcagc gtcagcaagt agaagcggct
     9361 gttctccggc agcgcgtcca ggccggggat tgccgccatc cagtgcatat ccgggaaggg
     9421 aatatcggcg atcccctggt cgccgccggt caccgcgtcc catttgaagc acaccgccca
     9481 gacgatctgc gaaaacgcca gcgtcagcat ggcgaaatag atcttggtca ggcgcacgca
     9541 gaaatagccg aatatcaggg agaatgccgc ggcgcccagc ccggccgcgg ggaacgcgat
     9601 ggcaaatggc cagccgcagg tcttcatcag gatgccgcag acataggcgc caatgccgaa
     9661 ataggcgacg tgtccgaacg acaccaaccc cgtggttccc agcagcaggt tcaggctgac
     9721 ggcgaacagc gcgtcgatgg cgatctgcgt ggcgaggaac gtgccgtagc gcgagccgat
     9781 ccagggcaac ggcagcatcg cgaggaagat cagcagcgtc cacggcacgc ggcggatcag
     9841 cggccgggcg gggtgaacca gcgggcgtga cagcggaaca ctcatcggat cggccgtcca
     9901 agcaggcccc atgggcgaac cacgagaacc acagccatca gcgcgaacac ggcgaagacc
     9961 gagaactgcg gaaagatcag gatgccaaat gacagcgtct ggccgtagat catcgcgccc
    10021 cagaacgtgc cccagaagga gccgaggccg ccaatcacca cgacgatgaa cgcgttgacg
    10081 atgatctgca cgtccatgcc cggcaccacg ctctcgatcg gcgtgacgag tgcgccggcc
    10141 aggccggcca ggaaggagct gacgacgaac atgcccgtgt agagcgcccc gacattggcg
    10201 ccgagcgcgc cgagcatctc gcggtcatag gccgccgcgc gcaggagccg tcccgccgag
    10261 gtcttgctga ggatcaggcc gccggcgatg gcgatcgccg ggccgaccag gatgatgaac
    10321 aggtcgtaga acggcagcgt ggtgcccaac agatgaaagc tgccgtccag caccggcggg
    10381 cgatgaaccg aaagctgatc ggtgccccag gtgaacttcg ccgcgtcgcc caggatcaac
    10441 acaagtgcgt aggtgaagag caactggtag agttcatcca cgccatacag atgccgcagc
    10501 agcagccgct ccaccacgcc accgagtatg gcaaccgaga cacccgcggc cagcgccgca
    10561 atccagaacg cgccgccggt cggctgcagc caggtcacca cctgccacgc catgtatgcg
    10621 ccgatcatgt agaacgaccc atgcgcgaaa ttcagcacgc gcatgatgcc gaacaccagc
    10681 gacagtcccg atgcgatcag gaacaggaac atcgccgtcg tcagtccgct gaagaactgc
    10741 gcggagatca gggagagggt catggcggca gctgcatggg gttgcctcga tcaggcgatg
    10801 ggcggcaggc ggggcgatga cgtgaaatcc tgcgtggcaa gtctgcgcaa atcggcgcta
    10861 acagtggccg ccgggcagcc gtacacgtcg tggccggaag gagcgtgtaa tccaatcccg
    10921 ctcctgtcac ggccgagctt gacccggcca tgacgttgcg gcggattgga cgacagtcct
    10981 gcagggccgc caccaacaac tggctgtggc agactcctta accggcttgc gactcatgag
    11041 tcctccccga caaggcgtgg gtggcgggcc ttcgcccgcc acgacgtgta atgcttcaga
    11101 gctctgcgtg tgcgcctgtt tcggcctcag tgcatcgccg gttccggatt gtagtagacg
    11161 gggttcttga tcaccgcgaa gggtacgctc ggatcctgcg tcatctcgcc ccaatattcg
    11221 cccgcatcgg cgtcatgcgt cttggaattg aaggtgcgcg gacccagtgg cgtctggtag
    11281 gtcattcccg ccatgaccgc ggcaaccttc gcatcgtcgg tggtccccgc cttggcgatt
    11341 ccctgggcga tcgcctcgat cacgacatag ccctggatcg cccagctcga ggcgtatttc
    11401 gtgcccatcg ccttcgccac cttcgcatcg aagaccttct ccgccgccgg ctggttggga
    11461 ccgtgccaga ccaccggatc gtaggtgttg gcccagatgc cgaaggggaa ggagctgccg
    11521 aggctctgcg tgacgtccac cgagccgacc tcaccgccgg tgatgacccg gttatgcacc
    11581 gccttgaaat agccaagcgg cgcaccctgc ttgacgaagc tgatgaagtc gccggcaaat
    11641 tcgtcgcaat acaccgcgtc gggatgcttg ctcatctgcg cggtgatgaa cggcgtgaag
    11701 ttcgactcgc ccagcttcgg ccattgctga tcgacgatgg tgatatccgg gcggagctgc
    11761 ttcagcttgg cgacgaaggc ggcgaccgca tcgtggccat aggcgtaatc cggtgcgacc
    11821 gtcgccacgg tcttcacgtc tttccaccgc gccatgatga tggcgccggc ctggccttcc
    11881 atcgtcgtat tggaggcgga gcggaagaca tagggatgga tgtgcgccgc gtcggtcagc
    11941 gccgaggtct tggccgaagg cgtgtcgaag acgacgtgat tctccttggc gatggtggaa
    12001 accgcgggcg cctcggcaga ggtaagcgtg ccggccagga attgtacgcc gttgcgtacg
    12061 atcagttcgc gcgcgagccg cacggcctcc gcggggtttg ccttgctgtc gcgcggcagc
    12121 agttccagct tgcgcccaag cacgccaccg gcggcgttga tatcggcaac cgcgagctcc
    12181 gcgcccttca agatagggac gccatacacg gctgtcgggc cggagagcgg gatcgggaag
    12241 ccgatcttga tcggctcctt ggcgtacgtg gcggcaggca tggcgagggc gaacgccatc
    12301 gccgccagcg cgtgacggat ttccataaca caacctccca tgctgccgga cagcattctg
    12361 gccgtccggt cttttatgta agcaaaccgt gacccggtgc cgtccggctt gtcaagcagc
    12421 aactcatcgg tgcgcgtgcc ggaggaggcg ggccgggtca gctcaatcgc ggatccggca
    12481 cgcgcgcggc gacaagcacg tccacagcgg acgcccccgc cgcgagcagt gtctccgcgc
    12541 aggcattcgc cgtggcgcct gacgtcatca catcgtcgat cagcagcacg cggcgctcgg
    12601 cgaccaccgg cctgcgtgat ggccgcaccg cgaaggcatc gcgcactgtt tccgcccgtt
    12661 ccgccgccga cttctcaccc agcgaatccg tcgccctgcg gcgttgcagc gcgtcaaccg
    12721 aggccggaac ccggctcaac cgggccagcg cctgcgccag cagcgcggcc tggttgtagc
    12781 ggcggcggaa cagccgcagc cggtgcaggg gcacgggcac cagcaactcg gcgcggttca
    12841 gcaaggccgc gccggcgcgc gccatcatcg gtgccaggat cgccgcaagg tccggccggt
    12901 cggcatgctt gaacggcaga atcaggcggc ggccctgctt gtcataccga agcgcggcgc
    12961 gggcctggcc gaagcgcggc ggatcggtct cgcagaccgg gcagagctgc cgaagcccgc
    13021 cctggcccgc attaccgaac ggcaccccgc agcgcaggca gtatggggcg gtgatgaagg
    13081 cggtggcgcg gaagcaggcg gcgcagaact ggcccggccg agacaccgcc gcatcgcagc
    13141 agatgcattg cggcggcagc agcaggtcga gcgtcgcacg tccggccgca ctggctccgg
    13201 cggcgagccg cggcagcaga ccggactcag gcagcagcgt ccgccacctc cacgccaaac
    13261 cgcacggtgc cgtcgcgttc gat
//
LOCUS       DASSZH010000038         2402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_256452, whole genome shotgun sequence.
ACCESSION   DASSZH010000038 DASSZH010000000
VERSION     DASSZH010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256452"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1445)
                     /locus_tag="VFN42_00715"
     CDS             complement(<1..1445)
                     /locus_tag="VFN42_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET8995164.1"
                     /translation="MPSRIEDYAMIGDGRTVALVSRTGALDWLCLPRFDSDPCCAALL
                     GTEDNGRWCISPCGPASTRRGYVDDTLVLQTEFTSDAGSARLTDFMAMGTETPTVIRK
                     LTGISGTMRMRCELSLRFDWGLMPPRIVRQDGALLAIVGPDLVALRAPVHLEQSEPGS
                     MTAEFELPAGAEVNFVLTYGTSHYPPPKPVDAGKAEADTLGFWRGWAATFQSKTPWRD
                     AVLRSLITLKGLIYHPCGGMVAAPTSSLPEMPGGTLNWDYRYSWLRDASFTLGALLNA
                     GYRDEAACWRDWMLRAIAGSPEKMRILYRVDGGRRMDEQKIEWLKGYEGAGPVRVGNS
                     AAAQRQIDVVGELLDALNLIARGGVKETPESLTAERELVEHLERTWQDKGHGLWESRG
                     EPHRYTYSMAMAWVGVDRFLRNNARRGTMDTATLEHLRALRDQIHAEVVELGWNHARG
                     HFVDRYGGERVDASLLLLPLVNFLPADDPRMA"
     gene            1541..>2402
                     /locus_tag="VFN42_00720"
     CDS             1541..>2402
                     /locus_tag="VFN42_00720"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HET8995165.1"
                     /translation="MAPPATPEETGCRPLPLVSLGECGIVALTIMAGWVTARFPSALP
                     AWAPWDFSWTAFLATGLALLWFLRGLARTPHAQRIAAWRCLCFLSGLAGFYAVLLTHF
                     EYLAQHAFFLNRVQHVAMHHLCPFLIALSWPGETMARGMPPRLLRALSSTPVHRLLRV
                     LQNPWLAYILFEGLLLLWLVPPVTFAAMIDPHLFAVMNASMVIDGLLFWFLVLDPRPP
                     EQAPLRFFTRLLLGFVVIFPQIAAGTVIGGARHDLYPSFALCGRVYTSIGPLLDQQIG
                     GLILWVPAGMM"
BASE COUNT          358 a          839 c          760 g          445 t
ORIGIN      
        1 gccatgcgcg gatcgtcggc cggcagaaaa ttcaccagcg gcagcagcag caggctggca
       61 tccacgcgtt caccgccgta gcgatcgacg aagtgaccgc gtgcgtggtt ccatccaagc
      121 tccaccacct cggcatggat ctgatcgcgc agtgcgcgca ggtgctccag ggtggcggta
      181 tccattgtgc cgcgccgagc gttgttgcgc aggaagcggt ccacgccaac ccaggccatg
      241 gccatggaat aggtatagcg atgcggttcg ccgcgcgatt cccacaatcc gtgtcccttg
      301 tcctgccagg tgcgctccag gtgctccacc agctcgcgtt cggccgtcag cgattccggg
      361 gtttccttca ccccgccgcg cgcgatcagg ttcagcgcgt ccagcagctc acccaccacg
      421 tcgatctgcc gctgcgccgc cgcactgttg cccacgcgca ccgggccggc gccctcatag
      481 cccttcagcc attcgatctt ttgctcgtcc atgcgccggc cgccatcgac gcgatacagg
      541 atgcgcatct tctctggaga accggcaatc gcgcgcaaca tccagtcgcg ccagcaggca
      601 gcctcatccc ggtagccggc gttcagcagc gcgccgaggg tgaagctggc atcgcgcagc
      661 caggaatagc gataatccca gttcagcgtg ccgccgggca tttccggcag agaactggtc
      721 ggcgccgcga ccatcccgcc acacgggtga tagatcagcc ccttcagcgt gatcagcgat
      781 cgcaggaccg cgtcgcgcca cggcgtcttg ctctggaacg tggcggccca gccgcgccag
      841 aagcccagtg tgtccgcctc ggcctttccg gcatctaccg gcttcggcgg cgggtaatgc
      901 gacgtgccat aggtcagcac gaaattcacc tctgctccgg ccggcagttc aaattccgcg
      961 gtcatgctgc ccggctcgga ttgctccagg tgcaccggcg cgcgcagcgc gaccaggtcg
     1021 ggaccgacga tggccagcag cgccccgtcc tgccgcacga tgcgcggcgg catcaggccc
     1081 cagtcgaagc gcagcgacag ttcgcaacgc atgcgcatgg tgccgctgat gccagtcagc
     1141 ttgcggatca cggtgggcgt ttccgtgccc atcgccatga aatcggtcaa acgagcgctg
     1201 ccggcgtcgc tggtgaactc ggtctgcagc accagtgtgt cgtccacata accgcggcgc
     1261 gtgctggcgg gcccgcaggg cgagatgcac caccggccat tgtcttcggt acccagcaag
     1321 gcggcgcaac agggatcgct gtcgaagcgc ggcaggcaca gccaatcgag cgcgccggtg
     1381 cgggatacca gtgcgaccgt gcggccgtcg ccgatcatcg catagtcctc gatccgtgat
     1441 ggcatcccgg ctctctcctt cgcttgctcc gcgcccagac ctgcaacggc ccaagcgaag
     1501 ccgcgttcac agcgggccgc ccgcgctcgg cacagaattg atggcgccgc ctgccacccc
     1561 ggaggaaacc ggttgccggc cgctgccgct cgtttccctt ggggaatgcg gcatcgtcgc
     1621 attgaccatc atggcaggct gggtgacggc ccgctttccg tcagcgcttc cggcctgggc
     1681 gccgtgggac ttctcctgga cggcctttct cgcaacgggg ctggccttgt tgtggtttct
     1741 gcgcggcctg gcgcggacgc cgcatgcgca gcgcatcgcg gcatggcgct gcctctgctt
     1801 cctgtccggc cttgcaggct tctacgccgt gctgctgacc catttcgaat atctcgccca
     1861 gcatgcgttc ttcctgaacc gggtacagca tgtggcgatg caccacctgt gcccgttcct
     1921 gattgcactg agctggccgg gtgagaccat ggcgcgcggc atgccgccaa ggctgctgcg
     1981 tgccctgagc agtacgccgg tgcatcgcct gctgcgggtg ctgcagaacc cctggctggc
     2041 gtacatcctg ttcgagggcc tgctgctgct gtggctggtt ccgccagtca ccttcgccgc
     2101 catgatcgac ccgcatctgt tcgcggtgat gaatgccagc atggtgattg acgggctgct
     2161 gttctggttc ctggtgctgg acccgcgtcc gccggaacag gcgccgctgc gcttcttcac
     2221 ccgcctgctg cttggattcg tcgtcatctt cccgcagatc gccgcgggca ccgtgatcgg
     2281 cggggctcgg cacgacctgt atccgtcctt cgccttgtgc ggccgcgtct atacgtcgat
     2341 cgggccactg ctcgaccagc agatcggcgg gctgatcctg tgggtgccgg ctggaatgat
     2401 ga
//
LOCUS       DASSZH010000039         2265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1176842, whole genome shotgun sequence.
ACCESSION   DASSZH010000039 DASSZH010000000
VERSION     DASSZH010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176842"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="VFN42_00725"
     CDS             complement(<1..993)
                     /locus_tag="VFN42_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HET8995166.1"
                     /translation="MTDPALLLLQLAGSVALLLWGVRMVQTGVQRAFGARLRTLLGTA
                     LRSTATSFLAGLGVTAALQSSTATGLMVTGFAAGGLVDLVPALAVMLGANVGTTLIVQ
                     LLSFDLSAVTPALILLGVLMFRRGQASRTHDLGRVFIGLGLMLLALHQLLDLVTPFET
                     APGLRTMLAAIARQPWLDVALAAGLTWAAHSSVAVVLLVMSLAAKGVVPPEAAFALVL
                     GANLGTAINPLLETTEGKDPAARRVPAGNLGTRLVGVAIGLALLPKIAAWLLVVAPNP
                     ARAVADLHLGFNLAVAALFLPVLPYYAVLLRRLLPAQVDAPDPSRPVYLDAAARE"
     gene            complement(1136..1711)
                     /locus_tag="VFN42_00730"
     CDS             complement(1136..1711)
                     /locus_tag="VFN42_00730"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET8995167.1"
                     /translation="MAGLVRSEEPDARMSRAEYRRWAESRHGRFERINGQVVAMAPER
                     VGHTRIKGEAYAALRAAVARAGLPCEVLTDGPTIEVGDSDYQPDAILRCGTASFSADR
                     LVVPDPVVIVEVLSPTTRRIDVSQKLADYFLVPTVQHYLILFADRIQAIHHRRLGDRI
                     DTQVLTSGEVRLDPPGIAVPLTRFYPAGTVA"
     gene            1864..>2265
                     /locus_tag="VFN42_00735"
     CDS             1864..>2265
                     /locus_tag="VFN42_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995168.1"
                     /translation="MHYRRISADCHLDMPWMPPTLFVDNASRELKDRMPYVADSPDGP
                     MWVTKNGASFGLKNGVGPSGAKLVPGQNRRVDIMAETGLFADGQKDIRRVSDPHLRIK
                     DMDRDGVDAEVIFGILGSASKLQDHAAAAEMF"
BASE COUNT          409 a          777 c          774 g          305 t
ORIGIN      
        1 ctcgcgggct gccgcatcca gatagaccgg gcgtgacgga tccggcgcgt ccacctgcgc
       61 gggcagcagg cggcggagca gcaccgcgta atagggcagc accggcagga acagcgcggc
      121 gacggccagg ttgaatccca gatgcaagtc ggccaccgcg cgtgccgggt tcggtgcgac
      181 aaccagcagc caggcagcga tcttcggcag cagggccaag ccgatcgcca cgccgacgag
      241 gcgcgtgccg aggtttccgg cgggcacccg ccgcgccgcc gggtccttgc cttcggtggt
      301 ttccagcaac ggattgatcg cggtgccgag attggcgccc agcaccaggg cgaacgcggc
      361 ttccggcggc accacgcctt tcgcggccag cgacatgacc agcagcacca ccgccacgga
      421 ggaatgcgcc gcccaggtca gcccggcggc gagcgccaca tccagccagg gctggcgcgc
      481 gatggcggcc agcatcgtgc gcaggccggg ggcggtttcg aatggcgtga caaggtccag
      541 caactggtgc agcgccagca gcatgagtcc caggccgatg aacacccgtc caaggtcgtg
      601 ggtgcggctc gcctgcccgc ggcggaacat cagcacgccc agcaggatca gcgccggggt
      661 gaccgcggac agatcgaacg agagaagctg cacgatcagc gtcgtgccga cattggcgcc
      721 cagcatcacg gccagcgcgg gcaccaggtc caccagcccg ccggcggcaa agccggtcac
      781 catcagtccg gtggcggtgc tgctctgcaa cgcggcggtg acgcccagcc cggccaggaa
      841 ggacgtggcg gtgctgcgca gggcggtgcc gagcagggta cgcaagcgcg cgccgaaggc
      901 acgctgcacg ccggtctgca ccatgcgcac gccccagagc agcagcgcaa cgctgcccgc
      961 gagctgcagc aacagcagag cggggtcagt catccagcat cgctttcctt ccgatccggg
     1021 gcgatccacg gcgtccggga catgttgatc ccgcccaaca acgctcggca agccgttccg
     1081 gaaccggtca ccccggcggc cgcggtctca acggcggcgg aagaacgccg gtggctcagg
     1141 cgaccgtgcc cgccggataa aatcgcgtga gtggaacggc gatacccggg ggatccagtc
     1201 tgacctcgcc gctcgtcaga acctgcgtgt cgatccgatc gcccagccgc cgatgatgaa
     1261 tcgcctggat gcggtcggcg aacaggatca ggtaatgctg caccgtcggc acaaggaaat
     1321 agtcggcgag cttctggctg acatcgatgc ggcgcgtggt cggcgagagg acttcgacga
     1381 tcaccacggg atccggcacg acgagtctgt cggcagaaaa agaagcagtg ccgcaccgca
     1441 ggatcgcatc cggctggtag tcgctgtcac ccacctcgat ggtcggcccg tccgtcagga
     1501 cctcgcaggg aaggccggcg cgcgcgacag ccgcccgcag tgccgcatac gcctcgccct
     1561 tgatccgcgt atggccgacg cgctccggcg ccatcgcgac cacctggccg ttgatgcgct
     1621 cgaaccggcc atggcggctt tcagcccagc gccggtactc tgcccggctc atccgggcgt
     1681 ccggttcctc gctccttacc agcccggcca tgacgaaatc tccgcgtctg cacggtgaag
     1741 gataggcata tggcccttca ccggcaatca acccagccgc ggcttgcagt gccgccgcac
     1801 tggattaggc tccccggcaa ggcagcggcg gtccagccgc tgtgcaaggg gagggaaacc
     1861 agcatgcact atcgtcgcat ctccgccgac tgccacctgg acatgccgtg gatgccgccc
     1921 acactgttcg tggacaatgc gtcacgcgag ttgaaggatc gcatgcccta tgtcgccgac
     1981 agcccggacg ggccgatgtg ggtgaccaaa aacggcgcca gtttcggcct gaagaacggc
     2041 gtcggtccca gcggcgccaa gctggtgcct gggcagaacc gccgggtgga tatcatggcg
     2101 gagaccgggc tgttcgccga cgggcagaag gacatccgcc gggtcagcga tccgcatctg
     2161 cgcatcaagg acatggaccg cgacggggtg gatgccgagg tgatcttcgg catcctcggt
     2221 tccgcgtcga agctgcagga ccacgccgcc gcggcggaga tgttc
//
LOCUS       DASSZH010000040         2533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_699635, whole genome shotgun sequence.
ACCESSION   DASSZH010000040 DASSZH010000000
VERSION     DASSZH010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699635"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VFN42_00740"
     CDS             complement(<1..518)
                     /locus_tag="VFN42_00740"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET8995169.1"
                     /translation="MHTSVLIVGGGPVGTTLALDLAWRGIDVIVAELRARGEPPSVKC
                     NHVSARTMEIFRRLGVAAAVRDTGLPADYPNDVAFRTTTTSIEFARIPIPCRRDRYTA
                     TGGPDTWWPTPEPPHRINQIYLEPVLVQHAAAQPDIRFLHRTKVLGFTQSDDGVLAEA
                     EDLDSGKRLLIR"
     gene            complement(643..2328)
                     /locus_tag="VFN42_00745"
     CDS             complement(643..2328)
                     /locus_tag="VFN42_00745"
                     /inference="COORDINATES: protein motif:HMM:NF006203.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HET8995170.1"
                     /translation="MTTPPRIAAEDYLDALAAQGIEHLFANPGTDFAPIVEAFARAAR
                     TNRKVPRPMVVPHENAAVGMAHGYTMVTGKPQAVMTHTNVGTANAINLLIDASRDRVP
                     MLLCSGRTPYTESGPSGARNAHIHWAQEMYDQAGMLREVVKWEYELTRGDQVSSVVER
                     ALEMATASPQGPVYIALPREVLGEELTADMHHKPRARPRPPVPAAADIDRLADWIAAA
                     RLPLIITGTLGRDPQEAVVLSRLAERYALPVIPYNTRYFAIGGNHPMFQGSSPRPLLD
                     NADLVIVLETDVPWLPSQENPPAGARVVQIGEDALYARYPMRSFPSDLTITASALPVL
                     QALEAALADRKCPDAPDRHTRLVERSRKLHAGWREEAERGGRGNTNTLPWLNHCLREV
                     VDRDTLVISEYSFRQEYCPLETPGSLFALSAAGGLGWGFPASLGAKLAAPEKQVVSVL
                     GDGAYMFANPTACHMVAQTQNLPVLTVVYNNTLYGAVRRATLGMYGHGAAAEGDGRLL
                     ADLPSPAFEKALPAYDGHGERVEHPADLPAALKRAAGAVRAGQQALVNVICPP"
BASE COUNT          355 a          856 c          901 g          421 t
ORIGIN      
        1 ctgcggatca gcaggcgctt gccgctgtcc aggtcttccg cttccgccag cacgccgtca
       61 tccgactggg tgaagccgag caccttggtt cggtgcagaa agcggatgtc cggctgcgcg
      121 gcggcgtgtt gcaccagcac cggctccagg tagatctggt tgatgcggtg cggcggttcc
      181 ggtgtcggcc accacgtatc cgggccgccg gtcgcggtgt agcggtcgcg ccggcagggg
      241 atcgggatgc gggcgaactc gatgctggtg gtggtggtgc ggaacgccac atcgttcgga
      301 taatctgccg gcagcccggt atcgcgcacc gctgcggcga cgccgagccg gcggaagatc
      361 tccatcgtgc gcgccgagac gtggttgcac ttcacgctgg gcggttcgcc gcgcgcacgc
      421 agttccgcga caatcacgtc gatgccgcgc caggcgaggt ccagggccag cgtggtgccc
      481 accggcccgc cgccgacgat cagcacgctg gtgtgcatca cgtgtttgcg tgcgacgtgt
      541 catggccggg cttggcccgg ccacccacgt tttgctgaca cacgcagaag aagccgtggg
      601 tgcccgggcc aggcccgggc atgacagcga aggggcgttg tttcacggcg ggcagatcac
      661 gttgaccagc gcctgttgcc cggcgcgcac cgcgcccgcg gcgcgtttca gtgccgcagg
      721 cagatcggcc ggatgctcca cccgctcgcc gtgaccgtca taggcgggca acgccttttc
      781 gaacgccggc gacggcaggt cggccagcag gcgtccatcg ccctccgccg ccgcgccgtg
      841 gccgtacatg cccagcgtcg cgcgccgcac cgcgccgtac agcgtgttgt tgtacaccac
      901 cgtcagcacc ggcaggttct gcgtctgcgc caccatgtgg catgctgtcg gattggcgaa
      961 catgtaggca ccatcaccga gcaccgacac cacctgcttt tccggcgccg ccagcttggc
     1021 gccgagcgag gcagggaaac cccagcccag cccgccggcg gcggacagtg cgaacaggct
     1081 gccgggcgtc tccagcgggc aatactcctg gcggaacgag tattcgctga tcaccagcgt
     1141 gtcgcggtcc accacctcgc gcaggcagtg gttcagccag ggcagcgtgt tggtgttgcc
     1201 gcgcccaccg cgttccgctt cctcccgcca gccggcgtgc agcttgcgcg aacgctcgac
     1261 caggcgggtg tgacggtcgg gcgcgtcggg acatttgcga tccgccagcg cggcttccag
     1321 cgcttgcagc accggcagtg cggacgcggt gatggtcagg tcgctgggga agctgcgcat
     1381 cggatagcgc gcgtacagcg catcctcgcc gatctgcacc acgcgcgcgc ccgctggtgg
     1441 gttctcctgg gacggcagcc aggggacatc ggtttctagc acgatcacca ggtcggcatt
     1501 gtccagcagc gggcggggcg acgatccctg gaacatcggg tgattgccgc cgatggcaaa
     1561 atagcgcgtg ttgtacggga tcaccggcag tgcgtagcgt tccgccaggc gagacagcac
     1621 caccgcttcc tgcggatcgc ggcccagcgt gccggtgatg atcagcggca ggcgggcggc
     1681 ggcaatccag tcggccagcc ggtcgatgtc agccgccgcg ggcaccggcg ggcgcggccg
     1741 ggcacggggc ttgtggtgca tgtccgcggt gagttcctcg cccagcacct cgcgcggcag
     1801 cgcgatatac accgggccct gcggcgaggc ggtggccatc tccagcgcgc gctccaccac
     1861 gctggagacc tggtcgccgc gggtgagttc gtactcccac ttcaccactt cgcgcagcat
     1921 gccggcctgg tcgtacatct cctgcgccca gtggatatgc gcgttgcgcg cgccggacgg
     1981 gccggattcg gtgtacggcg tgcggccgga gcacagcagc atcggcaccc ggtcgcggct
     2041 ggcgtcgatc agcaggttga tcgcattggc ggtgccgaca ttggtatgcg tcatcaccgc
     2101 ctgcggcttg ccggtgacca tggtgtagcc gtgcgccatg ccgaccgccg cgttttcgtg
     2161 cggcaccacc atcgggcgcg gcactttgcg gttggtgcgg gcggcgcggg cgaaggcctc
     2221 gacgatcggg gcgaagtcgg tgccggggtt ggcgaacagg tgctcgatgc cctgcgccgc
     2281 gagcgcgtcc aggtagtcct cggcggcgat gcgaggcggc gtggtcatgc ttggactcct
     2341 tgtcgttgcc cccgatcttt cgtgtgccgg ccgccgggtc aagagcggga cgggggccgc
     2401 ttgacccagg cggcgcggac tgctctgtcg ccggtatgga aagtgccaat cgcaccgtgc
     2461 atgtcggcga tatcgccatc gccaatcatc tgcctttcgt gctgatcgcc ggcccgtgcc
     2521 agatcgaatc ccg
//
LOCUS       DASSZH010000041         2527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1159921, whole genome shotgun sequence.
ACCESSION   DASSZH010000041 DASSZH010000000
VERSION     DASSZH010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159921"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VFN42_00750"
     CDS             complement(<1..814)
                     /locus_tag="VFN42_00750"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET8995171.1"
                     /translation="MSVMDRFLARFSPKAALRHGMRLSEKGAVKRAFPLLSRAAQAGL
                     AEAEYRVGRCYLEGAGVPVSRSEGARWLERAAGHGFVEAQALLATLYLHGLAAEAAGS
                     DPGSAANLFTSNTSANPPDFVGAEKWARRASEGGSAEGQALLGYILTSGPESMRNLPE
                     AFQWYERSARANCAQGHLGYALSLSNDATTPDKQKKVTEHLRAAADAGLPTALYLLGM
                     IAERGLGVAADLPAAIDYYRQAAEKGHRSAQARWGVALLEGRGVEANPTEGES"
     gene            complement(858..2270)
                     /locus_tag="VFN42_00755"
     CDS             complement(858..2270)
                     /locus_tag="VFN42_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HET8995172.1"
                     /translation="MPLLPQPSSKATSTALALAERNDPTLPAILEFQSPSTAVVNMPV
                     PRSARHTVWVIASMFAACITAMGLIKVDSVVTAHGEVVSRQPMLVVQPLETSIVRDID
                     VHVGEKVHKGQVLAHLDPTFAAADLSADAAQVSSLQAQVARMQAEVDNRPFTYSGLDP
                     DLSLQAAIYAQRQSEYHYKLENYKQKADSLLASIQRAKADETGYKQRLAVATDVENMR
                     KQLEHLHVGSRLNTLAAMDNRAEMARDVASAQQQAAAAARDLAALVAERDAYVENWHS
                     EIAQKLADATAKLSDAREQMNKAQLRRQLVELRADRDATVLTVAKVSVGSVLQSGQQL
                     ITLVPSDAPLEVEANIAGRDNGYVHVGDPVAVKFDTFPFTKYGLARGTVRVISADSFT
                     AQEDQRNPTSQIPVPNSGEPYYRARIAIDKVELHGVPKGFHLMPGMPVTSDVKVGKQT
                     VLSYLLSEIMPVGMDAMREP"
     gene            complement(2233..>2527)
                     /locus_tag="VFN42_00760"
     CDS             complement(2233..>2527)
                     /locus_tag="VFN42_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003630137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HET8995173.1"
                     /translation="DEATSALDPESEALVNANLLRIARGRTMVIVSHRLSSLTECDQI
                     LVLEKGKVMDCAPHRVLLERCTVYRQLWAQQTRYMETQGPRHAAPAPALVQGD"
BASE COUNT          378 a          851 c          855 g          443 t
ORIGIN      
        1 aggactcgcc ttcggtagga ttggcttcga cgccgcgccc ttccagcagc gccacgcccc
       61 agcgggcctg cgcactgcgg tgccccttct ccgccgcctg gcggtagtag tcgatggccg
      121 cgggcaaatc cgccgcgacg ccgaggccgc gctcggcgat catgcccagc aggtacagcg
      181 cggtgggcag tccggcatcg gcggcggcgc gcaggtgctc ggtgaccttt ttctgcttgt
      241 cgggggtagt cgcgtcattc gacagcgaca gcgcgtaacc gagatgacct tgcgcgcaat
      301 tcgccctggc ggagcgctca taccactgga acgcctcggg caggttgcgc atggactccg
      361 gaccagaggt caggatgtag ccgagcagtg cctgcccctc ggcggagccg ccttccgagg
      421 cgcggcgggc ccatttctcg gccccgacga aatccggcgg gttggcgctg gtgttgctgg
      481 tgaacaggtt ggcggcggag ccaggatcgc tcccggcggc ttccgccgcc aggccgtgca
      541 ggtacagcgt ggccagcagc gcctgtgcct ccacgaaacc atggccggcg gcacgttcca
      601 gccagcgcgc gccctctgaa cggctgactg gcaccccggc gccttccaga tagcagcgcc
      661 cgacccggta ttcggcttcg gcgaggccgg cctgcgcggc gcgcgacaac aggggaaagg
      721 cgcgtttcac cgcgcctttc tcggatagcc gcatgccgtg gcgcagcgct gccttgggcg
      781 agaaccgggc gaggaaccgg tccatcacgc tcatgccacc cccgcccagg ccaggaccgg
      841 cttgccgtct ccccgcatca tggctcccgc atcgcgtcca ttccgaccgg catgatctcg
      901 ctgagcagat agctcaacac cgtctgcttg ccgaccttca cgtcggaggt gaccggcatg
      961 ccgggcatca ggtggaagcc tttcggcacg ccgtgcagct ccactttgtc gatggcgatg
     1021 cgcgcccggt agtacggctc gccgctgttc ggcaccggga tctggctcgt cgggttgcgc
     1081 tgatcctcct gcgcggtgaa gctgtcggcg ctgatgaccc gaaccgtgcc ccgcgcgagg
     1141 ccgtatttcg tgaaggggaa agtgtcgaac ttgaccgcca ccggatcgcc cacgtgcacg
     1201 tagccgttgt cgcggcccgc gatattggct tccacctcca gcggcgcatc ggacggcacc
     1261 agggtgatca gttgctggcc agactgcagc acggaaccaa ccgatacctt ggccaccgtc
     1321 agcaccgtgg cgtcacggtc ggcgcgcagt tcgaccaact gccggcgaag ctgcgccttg
     1381 ttcatctgct cgcgcgcgtc ggacagcttg gcggtggcat cggccagctt ctgcgcgatc
     1441 tcggaatgcc agttctcgac ataggcgtca cgttcggcaa ccagcgccgc caggtcgcgc
     1501 gccgccgcgg ccgcctgctg ctgcgcactg gccacatcgc gcgccatctc ggcccggttg
     1561 tccatggcgg cgagcgtgtt cagccggctg ccgacatgca gatgctcaag ctgcttgcgc
     1621 atgttttcga catcggtcgc caccgcgagg cgttgcttgt aaccggtttc gtccgccttg
     1681 gcgcgctgga tggaggcaag caggctatcg gccttctgct tatagttctc cagtttgtag
     1741 tggtactcgg actggcgctg ggcatagatc gccgcctgca gcgacaggtc gggatccagg
     1801 ccggaatagg tgaagggccg gttgtccact tccgcctgca tccgcgcgac ctgcgcctgc
     1861 aggctggaca cctgtgcggc gtccgcggac aggtcggccg cggcaaaggt cgggtcgaga
     1921 tgggccagga cctggccctt gtgcaccttc tcgccgacat gcacgtcgat gtcgcgcacg
     1981 atggaggttt ccagcggctg taccaccagc atcggctggc gcgagaccac ctcgccatgc
     2041 gcggtgacca cgctgtccac cttgatcagg cccatcgcgg tgatgcacgc ggcgaacatg
     2101 ctggcgatga cccacaccgt gtgccgagcg gagcgcggca ccggcatgtt gaccaccgcg
     2161 gtggacggcg actggaactc caggatcgcc ggcagcgtcg ggtcattccg ctcggcgagg
     2221 gcgagggcgg tactagtcgc cttggacgag ggctggggca ggagcggcat ggcggggacc
     2281 ttgagtttcc atatagcggg tctgctgcgc ccaaagctgg cgatacacgg tgcagcgttc
     2341 gagcaggacg cggtgtggcg cgcagtccat caccttgccc ttctccagca ccaggatctg
     2401 gtcgcattcg gtgagcgagg acaggcgatg tgacacgatc accatcgtcc gtccgcgcgc
     2461 gatgcgcagc aggttggcgt tgaccagcgc ttcggattcg gggtccagtg cgctggtggc
     2521 ctcatcg
//
LOCUS       DASSZH010000042         4261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_34890, whole genome shotgun sequence.
ACCESSION   DASSZH010000042 DASSZH010000000
VERSION     DASSZH010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34890"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VFN42_00765"
     CDS             <1..618
                     /locus_tag="VFN42_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET8995174.1"
                     /translation="LRKLHAAGISRAVLAVNHMRHLIEAFFGDGSRFGLPITYSYEDR
                     PLGTAGPMGAVLDQLGDDFLLTNGDLLTTLDIGRMIAEHRSRQADATIGVFEREVKID
                     FGLIDVDADMRMTGYREKPSHRHLVSMGIYVLRAAAVRGFVHAGEHLDMPDLMQAMMQ
                     AGRRVFCHQQDCIWLDIGRPDDFALAQQMVAEQPGRFLGVEGQPA"
     gene            615..1514
                     /locus_tag="VFN42_00770"
     CDS             615..1514
                     /locus_tag="VFN42_00770"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET8995175.1"
                     /translation="MSQRALVTGATGFVGRHLVAELQSAGWQVVRAVRAEPDRTPPGT
                     LGLGCDPWTTATFAGALEQARPDVVFHMAGLTWADAPAAFYAANVMLAAHLLDAVAAS
                     DTRPAVVLAGSAAEYGYVPNSLLPVREDAPCNPVTHNGISKYAQTLLGLAHARAGLRV
                     LVARIFNPVGTGMPQRLALASFARQLRDGCDSLTVGNLDVARDFIDVAEAARLIVALA
                     GRPESDGQVINICSGVAFVLRPLVETMIRLTRRDVRLDVAPALLRPGEMRAFLGSTAR
                     LSAAGLTVQPPDFSRILPALLAG"
     gene            1615..1833
                     /locus_tag="VFN42_00775"
     CDS             1615..1833
                     /locus_tag="VFN42_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995176.1"
                     /translation="MEDAFKPFGQSMELKSRVLGWLWQDDAAANLVLPDACDPTAGGT
                     RGTPPVQAAARRRWALPLPAFLLARRCP"
     gene            1904..2317
                     /locus_tag="VFN42_00780"
     CDS             1904..2317
                     /locus_tag="VFN42_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6455 family protein"
                     /protein_id="HET8995177.1"
                     /translation="METSPAASEPGLLARAIEWIKAYNARQQELGRLSRDDIDMMASD
                     LGMSAADLCDVLPRSADNTFLMDSMLLARGLDPDEVRRSLTPLLRDLELACTRCAATG
                     RCKRELLAGTAAMHSHQFCPNADTIDDLLEERANT"
     gene            complement(2356..3255)
                     /locus_tag="VFN42_00785"
     CDS             complement(2356..3255)
                     /locus_tag="VFN42_00785"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HET8995178.1"
                     /translation="MSISIRPLHPTFAGAVTGVDLTHPLSREEVAAIEAGMDQYAVLV
                     FHDQPITDEQQCEFSRNFGELEVTLSGQMAKPEERRFGDRLELGDISNLDGVNKLRAR
                     DDRRRMYALANQLWHSDASFRAIGAGYTLLHARVVPSQGGNTEFADMRAAYDALDETT
                     KGEIADLITEHSITFSREQLGFTVESAGNEDKLRPVRHRLVLTHPVTGRKSLYLSSHI
                     GGIVGWPVPEARAFIRDLAEHATQRQFVYAHEWRVNDLVMWDNRQVMHRARRFNDLQE
                     VRDLRRTSIKGTGLLLEDTAAAA"
     gene            complement(3268..4158)
                     /locus_tag="VFN42_00790"
     CDS             complement(3268..4158)
                     /locus_tag="VFN42_00790"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HET8995179.1"
                     /translation="MSIAIQPLHPGFVGEVSGIDLTRALSRDEVAAIDAGMDRYAVLV
                     FHDQHFDDATQLAFSRNFGELEISAGGEMSKPEERRLAIEFADISNLDRDSRLRRADD
                     KRRLNALGNRLWHSDASFRAVPAKYSLLSGRIVPPSGGNTEFADMRAAYDALDDATKA
                     EIEDLVTEHSSAYSREQIGFPPVDFGADNQDKLRPVRHRLVRTHPVTGRKSLYLSAHI
                     GSIVGWPVPEARAFIRDLTEHATQPRFVHAHRWRQWDLVMWDNRTTMHRARRYDDLHD
                     IRDLRRTTIRGDGITVAQAA"
BASE COUNT          683 a         1376 c         1448 g          754 t
ORIGIN      
        1 ctgcgcaagt tgcacgcagc cggaatcagc cgggccgtgc tggccgtgaa ccatatgcgc
       61 cacctgatcg aggcgttctt cggcgacggc agccggttcg gcctgccgat tacctacagc
      121 tatgaagacc ggccgcttgg caccgccggc cccatgggcg cggtgctcga ccagttgggc
      181 gacgacttcc tgctcaccaa tggcgacctg ctcaccacgc tggatatcgg ccgaatgatc
      241 gccgagcatc gttccaggca ggccgatgct accattggcg tgttcgaacg tgaggtgaag
      301 atcgacttcg gcctgatcga cgtcgatgcg gacatgcgca tgaccggcta tcgggaaaag
      361 cccagtcacc gccacctggt cagcatgggg atctatgtgc tgcgcgccgc cgcggtgcgc
      421 gggtttgtgc atgcaggcga gcacctggac atgccggacc tgatgcaggc gatgatgcag
      481 gccggccgcc gggtgttctg ccaccagcag gactgcatct ggctggacat cggccggccc
      541 gacgacttcg ccctggcgca gcagatggtt gccgaacagc cgggacgttt cctcggcgtg
      601 gaaggacagc cggcgtgagc cagcgggctc tggtgaccgg cgcgacaggt tttgtcggcc
      661 ggcatctggt agccgaactg caatccgccg gctggcaggt ggttcgcgcg gtgcgagccg
      721 agccggatcg cacaccgcct ggaacgcttg gccttggatg cgatccctgg acgaccgcga
      781 cctttgccgg cgcgcttgag caggctcggc cggatgtcgt gttccatatg gcgggcctga
      841 cctgggccga tgcgccggcg gcgttctatg ccgccaacgt gatgctggcg gcgcatctgc
      901 tggatgcggt tgccgccagc gatacccgcc cggcggtcgt gctggccgga tcggccgccg
      961 agtatggtta cgtaccgaat tctctgctgc ccgtgcggga ggacgcacca tgcaatcccg
     1021 tcacgcataa cggcatcagc aaatacgcac agaccctgct tggactggcg catgcccgcg
     1081 ccggtctgcg cgtgctggtg gcgcgcatct tcaatccggt tggcaccggc atgccgcaac
     1141 gtctggcgct ggccagtttc gccaggcagc ttagggatgg atgtgacagc ctgacggtgg
     1201 gcaatctcga cgtcgcgcgg gatttcatcg acgtggcgga ggccgcgcgg ctgatcgtcg
     1261 ccctggccgg ccggccggaa agcgatggac aggtcatcaa tatctgctcc ggagtggcct
     1321 tcgtgctgcg cccgctggtg gagacgatga ttcggctgac ccggcgcgac gtccggttgg
     1381 atgtagcgcc ggccttgctg cgcccgggag agatgcgcgc gttcctcggc agtaccgcgc
     1441 ggctgtccgc ggcaggattg acggtgcaac caccggattt ttcacgcatc ctgccggcct
     1501 tgctcgccgg atgatggtcc gccgggtaaa tagcccgtga tcgactgaga gtatgatgtt
     1561 gtgcgctccg gcgtcccact aggttcaccg gcattcggta gcgcatggca ggatatggaa
     1621 gacgccttca agcccttcgg gcagtcgatg gaactgaagt cacgggtact cggctggctg
     1681 tggcaggacg acgccgccgc gaacttggtg ctgcccgacg catgtgatcc gacggccggc
     1741 ggcacgcgcg gcaccccgcc ggtccaggcg gccgcccggc ggcgttgggc cttgcccctg
     1801 ccggccttcc tgctggcgcg ccgttgtccc tgaggcgttg atccagatca aagcggactt
     1861 ccgccaatgc tgcatagctc atgcagcgcc acaggaggtc cctatggaaa cgtcccccgc
     1921 cgcaagcgaa cccgggctgc tggctcgggc gatcgagtgg atcaaggcgt acaacgcccg
     1981 ccaacaggaa cttgggcgct tgtcgcgcga cgacatcgat atgatggcga gcgatctggg
     2041 catgagcgca gccgatctgt gcgatgtcct gccgcggagt gcggacaaca ccttcctgat
     2101 ggacagcatg ttgctggcgc gcggcctgga tccggacgag gtgcgccgca gcctgacacc
     2161 gctgctgcgc gacctggaac tcgcctgcac gcgctgtgca gcaaccggac gctgcaagcg
     2221 cgaattgctg gccggcaccg cggcgatgca cagccaccag ttctgcccga acgccgatac
     2281 gatcgacgac ctgctggaag agcgggcgaa cacctgattc cggcgcgggc gaattctgaa
     2341 accgggaaga cgccgtcagg cggcggccgc ggtgtcttcc agcaacagcc cagtgccttt
     2401 gatcgacgtg cgccgcaggt cacgtacctc ctgcaggtcg ttgaaccggc gggcgcggtg
     2461 catcacctgg cggttgtccc acatcaccag gtcgttcacc cgccattcat gcgcgtaaac
     2521 gaactgccgc tgggtcgcat gctcggccag gtcgcggatg aaggcccgtg cctccggcac
     2581 cggccagccg acgatgccgc cgatatgcga tgacaggtac agcgacttcc gtccggtcac
     2641 cggatgcgtc agcaccaggc ggtggcgcac cgggcgcagc ttgtcctcat tcccggcact
     2701 ttccacggtg aagccgagct gttcgcggga aaaggtaatg gagtgttcgg tgatgaggtc
     2761 tgcgatctcg cccttcgtcg tctcgtccag cgcatcatac gcggcccgca tatcggcaaa
     2821 ctcggtgttg ccgccttggc tcggcacgac gcgcgcatgg agcagcgtgt agccggcgcc
     2881 gatcgcacgg aacgacgcgt ccgaatgcca tagctggttg gccagcgcgt acatgcgccg
     2941 gcgatcgtca cgtgcgcgca gcttgttcac cccgtccagg ttggagatgt cgcccagttc
     3001 cagccggtcg ccaaaccggc gctcctccgg cttggccatc tgtccggaaa gcgtaacctc
     3061 cagctcgccg aaattgcggc tgaactcgca ttgctgctcg tcggtgatcg gctggtcgtg
     3121 gaacaccaga acggcgtact gatccatgcc ggcctcgatc gccgcgacct cctcccgcga
     3181 cagggggtgg gtcaggtcca cgccagtcac cgcgccggcg aaggtgggat gcagcggtcg
     3241 gatgctgatg ctcatcggag ttcctcctta tgccgcttgt gcgacggtga tcccgtcgcc
     3301 acggatggtg gtgcgccgca ggtcgcgtat gtcgtgcagg tcgtcgtagc ggcgggcgcg
     3361 gtgcatggtg gtgcgattgt cccacatcac caggtcccat tgccgccagc gatgcgcatg
     3421 cacgaagcgc ggctgcgtgg cgtgctcggt caggtcgcgg atgaaggcgc gcgcttccgg
     3481 caccggccag ccgacgatgc tgccgatatg tgccgagagg taaagcgact tgcggccggt
     3541 caccggatgc gtgcgcacca gccggtgccg caccgggcgc agcttgtcct ggttgtcggc
     3601 gccgaaatca accgggggaa agccgatctg ttcgcgcgaa tacgcgctgg agtgttcggt
     3661 gaccaggtcc tcgatctccg ccttggtcgc gtcatccagc gcgtcgtagg cggcgcgcat
     3721 gtcggcgaat tccgtgttgc cgccgctggg cgggacgatg cggccggaca gcagcgagta
     3781 cttcgccgga acggcgcgga acgatgcatc ggaatgccac agccggttgc ccagcgcgtt
     3841 cagccgccgc ttgtcgtccg cgcggcgcag gcggctgtcg cggtccaggt tggagatatc
     3901 ggcgaactcg atcgccaggc ggcgctcctc cggcttgctc atctcgccgc cggcggaaat
     3961 ttccagttcg ccgaaattgc ggctgaacgc gagttgcgtt gcgtcgtcga agtgctggtc
     4021 gtggaacacc agcacggcgt agcgatccat gccggcgtct atggccgcga cttcatcgcg
     4081 cgacagcgcg cgggtgaggt cgatgccgga aacttcgccg acaaagccgg gatgcagcgg
     4141 ctgaatggca atcgacatgc acggtgctcc tcgaactccc ggcgtggcga cgccccccgt
     4201 gtcatggccg ggctttgccc gaccacccac gttttgccgt aaaagacagc ggaagacgtg
     4261 g
//
LOCUS       DASSZH010000043         8142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_324824, whole genome shotgun sequence.
ACCESSION   DASSZH010000043 DASSZH010000000
VERSION     DASSZH010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8142
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324824"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="VFN42_00795"
     CDS             <1..1131
                     /locus_tag="VFN42_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HET8995180.1"
                     /translation="DRRRISHIPEGGPENIDEDAALERLDKALEESVDLHQRSDVPYG
                     MFLSGGIDSSAIIALMARLNSEPVLAFTAGFDVPGAADEREHAAAVAKAVGAHHESIE
                     ITESMVWQHLPEIVGCMDDPTADYAIIPTWFLARRARQDVKVVLSGEGGDEIFAGYPK
                     YASAMRPWWFGGRAMYGRGSFDLVSNVLRTRPTTWRDGIVAAEAQAAAGGRSRLAAAQ
                     VTDMVDWLPHDLLLKLDRCLMAHAVEGRTPFLDPGVAAAAFRLPDAMKLRQGVGKWIL
                     RKWLEKHLPSARPFAPKQGFTVPVGQWIKGQGTRLGPLVAAYPGVAEIAHPDRVNALF
                     RGIRRWRHGIAAWKLLFYALWHRQHILGLPPAGDVFETLAAT"
     gene            1158..1388
                     /locus_tag="VFN42_00800"
     CDS             1158..1388
                     /locus_tag="VFN42_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995181.1"
                     /translation="MPADPSPVPRRSAPPAARALGIGAVGAIALGAVAVGALAIGRLA
                     IGRLAVGRLSAGHLRVRRLEVGELKIGRIVRD"
     gene            1406..2083
                     /locus_tag="VFN42_00805"
     CDS             1406..2083
                     /locus_tag="VFN42_00805"
                     /inference="COORDINATES: protein motif:HMM:NF041646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VC0807 family protein"
                     /protein_id="HET8995182.1"
                     /translation="MSESSGQPEAAAAADSPAAAERPTPSRWALARDMGINVVAPYAT
                     YLLLRHEGVSLVWSLAAGSIFPLAATLVGFARSRRVDALGIIVLVVTAASIIGAVLFT
                     SPYLLLAKGSLITGGIGTLFLLSLLMQRPLIYHLASNTGQDPEARAEYVAMWDSEPAF
                     RHLMRRLTLMWGLGMYVEASLRLLLIGRLPVAVFLPVSEAMWILFFALMTLWSWRYAS
                     RRMPEEA"
     gene            2273..3571
                     /locus_tag="VFN42_00810"
     CDS             2273..3571
                     /locus_tag="VFN42_00810"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HET8995183.1"
                     /translation="MMPALAPSADAGEEAAATPPHLRECRDCGQIQVVPAMPPSARAV
                     CLRCNAVLRHTHHEPLMAPLALHTAALVMLAISAGTMLLSVSSFGMYQAASLFSGPAG
                     LEQHGMWGLALLVLLTTFVIPLLKLLAVSAVLIGLQLPRPPGWLRRLFAWADRLRPWS
                     MIEIYLLGLFVAYSRLGKVVTVDIGPAVYALGALMLTMVAADYALDRQAVWEAMERRG
                     LHRAPPRRTGSEGPPVRLGCDTCGLVSRAEPGSPCPRCASALHHRKPFSIARTWALGL
                     SALILYVPANYYPVLTVIRLGWGQPSTIIGGVEELIAAGLWPLAALVFFASIVVPGLK
                     LIGLGVLLISTQRGSRRRLRDRTRLYRIVDSIGRWSMIDIFMGSILVALLQFGIIARV
                     TPGFGAVAFAAVVVLTMLAAQSFDPRLMWDAAGVQAEYQE"
     gene            3568..5262
                     /locus_tag="VFN42_00815"
     CDS             3568..5262
                     /locus_tag="VFN42_00815"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HET8995184.1"
                     /translation="MSDQPHSPGPPPHASVRSKVDRRWRLSLIWAIPIVTLALGLWLA
                     WHTMESRGPIIHISFESAEGLVAGQSHVKHKDVDMGVVQNIALSKNLQHVVVTVRMTR
                     EAEPLLTDRTKFWVVKPRFFAGALSGLETLVSGSYIEMLPSPKPGERTRNFTGLETPP
                     VLQSDVPGKTFLLHAPRLGSISLGSPVFYRDLDVGEVLGWDIGDMASTVTVHAFVRAP
                     YDKYVHDNSRFWNASGAKISLGGNGVQLQLESLRAVVLGGIAFETPDQGKGSPVTRNL
                     HEFPLYPDKQAADSANFAQRISCITYLTGSVAGLAPGASVTLHGLPVGQVESVMLKYD
                     KKHDTVVVPVRFDLEPGRVSGLTLPSGSGLDAEMRDVVRRGLRVSLESTNLITGQKQL
                     AIALHPDAPPATLEKQDGAYVIPVAPGGSGGDLSGSATELLAKLNAIPFGQIGHNLNQ
                     LIAGANGLVNNSGLQKAVGSLQGTMGELQGLVHRLNTASGPLVKDLPGMARQLDGAVK
                     QLNGLAASMQQGYGSNSDLHNQLSRLLAQLNDTAQSFRAFADLLQRHPEALIRGRK"
     gene            5277..5831
                     /locus_tag="VFN42_00820"
     CDS             5277..5831
                     /locus_tag="VFN42_00820"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HET8995185.1"
                     /translation="MPLTRRHALVLAALLPAACASPNPALFTLAAEPGQTQGNGPKLV
                     KVREVSLPHYMERSEIVRSTENYQLVVLPNDWWGESLSSMMNRVLVQDLSQRLPDSTV
                     VSEIGAISGTPNAAVAINVLRFDADRSGAVVLDAEIAVNGHPHGNRRVHLTVPLAGPG
                     TPALVSAMSAAIGKLADIVARMLA"
     gene            6222..7142
                     /locus_tag="VFN42_00825"
     CDS             6222..7142
                     /locus_tag="VFN42_00825"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET8995186.1"
                     /translation="MSAGQYALSLESAVADERPRRRAWHRHLHGSDLAWSIAFVLPYA
                     AVFVGFVIYPILYALWLGRDPALYSLLLTNPKFLEAAVNTAVFVGVAVNAQMFLALLL
                     SGFFARRRRWIKMLLVLFMLPWTLPAVPAYLSFHWMLVTGHLGLIDHLLQVLFGIEGP
                     NWFIHGWLGLTCNMVASVWKWLPLWTLILLGGRMAIPQDLYDAADIDGASLYHRFVHI
                     TVPLLANLYLISTLISAIWTFGDDTPVLFVSGGGPAFASSVVSLLSFHYAFDLAQPTL
                     GVAAGLCMLPLLIPLIVLLTRALHRTEVQL"
     gene            7151..8029
                     /locus_tag="VFN42_00830"
     CDS             7151..8029
                     /locus_tag="VFN42_00830"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET8995187.1"
                     /translation="MAEKRTRRRRNYRLRRTLVEGSSILLGAAIVIWSLIPVYNMFLV
                     ALDPLGHNEFSGLLWPEHPTLEVFANLWTEGADDVEGIWRQFGNSIYIGAVATALTVL
                     ISSMASFAIGRMRLGRQWTITGTALLTYAIPASVLIIPFIRIMQIYGLSDNLWAVIAA
                     EITFATPYAILVLLYYAKLIPAELDDAARIDGASVVQIYFRIYLPLMAPALAAVGTYA
                     LLYAWNDYFYQYMLLMSEKHTTVAVALEQFFDDDDAPWNYMMAIAIIYSLPPIATFYA
                     LRRFMATGLTAGGMKG"
BASE COUNT         1232 a         2741 c         2743 g         1426 t
ORIGIN      
        1 gatcgccgcc gcatctcgca cattccggaa ggcggcccgg agaacatcga cgaggacgcc
       61 gcactggaac gcctggacaa ggcgctggaa gagagcgtcg atctgcacca gcgcagcgac
      121 gtgccgtacg gcatgttcct gtccggcggc atcgacagct ccgccatcat cgcgctgatg
      181 gcccggctga acagcgagcc ggtgctggcc ttcaccgccg ggttcgacgt gccgggtgcg
      241 gcggacgagc gcgaacacgc cgccgcggtg gccaaggcgg tcggcgcgca ccacgaatcg
      301 atcgagatca ccgaaagcat ggtctggcag catctgccgg agatcgtcgg ctgcatggac
      361 gatcccaccg cggattacgc gatcatcccc acctggttcc tggcccgccg cgcccggcag
      421 gacgtgaagg tggtgctgag cggcgaaggc ggtgacgaga ttttcgccgg ctatccgaaa
      481 tacgccagcg cgatgcggcc ctggtggttc ggcggccggg ccatgtacgg ccgcggcagc
      541 ttcgacctgg tcagcaatgt gctgcgcacc cggcccacca cgtggcggga cggcatcgtg
      601 gcggccgagg cgcaggccgc cgccggcggg cgttcccgcc tggccgcggc gcaggtcacc
      661 gacatggtgg actggctgcc gcacgacctg ctgctgaagc tggaccgctg cctgatggcg
      721 catgcggtgg agggacgcac ccccttcctc gatcccggcg tcgccgcagc ggcgttccgc
      781 ctgccggacg ccatgaagct gcggcagggc gtgggcaaat ggatcctgcg caaatggctg
      841 gagaagcatc tgccgagcgc ccggcccttc gcccccaagc agggctttac ggtgccggtg
      901 gggcaatgga tcaagggcca gggaacccgg cttggccccc tggtggcggc ctacccgggg
      961 gtggcggaga tcgcccatcc ggaccgggtc aacgcgctgt tccgcggcat ccgccgctgg
     1021 cgtcacggca tcgccgcctg gaagctgctg ttctacgcgc tgtggcaccg ccagcacata
     1081 ctgggtctgc ccccggcggg ggatgtattc gaaaccctgg cggccacctg aagccggacg
     1141 caatcgcgga gggttccatg cctgctgatc catccccggt gccgcggcgc tcggcaccgc
     1201 cggctgcgcg tgcccttggc atcggcgccg tcggcgcgat agcgctgggc gctgttgccg
     1261 tcggcgcgct ggccatcggc cgcctggcca tcggccggct tgccgtcggc cgcttgagcg
     1321 ccggccatct tcgggtgcgg cgcctggagg tgggggaact caagatcggc cgtatcgtcc
     1381 gcgactgaag aatcggagca tgccaatgtc ggaatcaagc ggtcagccgg aggcagcggc
     1441 cgcagcggac agccccgcgg cggcggaacg cccgaccccg agccgctggg cgctggcccg
     1501 cgacatgggg atcaacgtgg tggcgcccta cgccacctat ctgctgctgc ggcatgaagg
     1561 ggtcagcctg gtctggtccc tggccgccgg ctcgatcttc cccctggcgg ccaccctggt
     1621 ggggttcgcc cgcagccggc gggtcgacgc cctgggcatc attgtcctgg tggtcaccgc
     1681 cgccagcatc atcggcgcgg tgctgttcac cagcccgtac ctgctgctgg cgaaaggctc
     1741 gctgatcact ggcggcatcg gcacgctgtt cctgctgtcg ctgctgatgc agcggccgct
     1801 gatctatcac ctggccagca acaccgggca ggacccggag gcacgcgccg aatacgtcgc
     1861 gatgtgggac agcgagccgg cgtttcgcca cctgatgcgg cggctgacgc tgatgtgggg
     1921 cctgggcatg tatgtggagg cgtcgctgcg cctgctgctg atcggccgcc tgccggttgc
     1981 ggtgttcctg ccggtcagcg aggcgatgtg gatcctgttc ttcgccctga tgaccctgtg
     2041 gagctggcgc tacgcgtcca ggcgcatgcc cgaagaggcc tgatcccgcc cggccgttca
     2101 cccgcgaggg cgaaagctga tccctgcctg ctggacatgg cgcgaccagc gcatgggcgt
     2161 cagcaccggg cagcgagatt ccagcgcgca cggcctatct atcggcaatg ccgccttgcg
     2221 cccctagata agcgcccatg tcgtccatca gcgccgaatc cggcgccacc ccatgatgcc
     2281 ggcccttgct ccctccgccg acgcaggcga ggaagccgcc gccacgcccc cgcatctgcg
     2341 ggagtgccgc gactgcggcc agatccaggt ggtgccggcc atgccaccca gcgcgcgggc
     2401 ggtgtgcctg cgctgcaatg ccgtgctccg ccatacccat cacgaaccgt tgatggcgcc
     2461 cctggccctg cataccgccg cgctggtcat gctcgccatc tcagccggca ccatgctgct
     2521 gtcggtcagc agtttcggca tgtatcaagc ggccagcctg ttcagcggcc ccgccgggct
     2581 ggaacagcat gggatgtggg ggctggccct gctggtactg ctgaccacct tcgtcatccc
     2641 gctgctcaag ctgctcgccg tcagcgccgt cctgatcggc ctgcaactgc cgcggccgcc
     2701 cggctggctg cgccggctgt tcgcctgggc cgaccggctg cggccctggt cgatgatcga
     2761 gatctacctg ctgggcctgt tcgtcgccta tagccgcctg ggcaaggtgg tgacggtcga
     2821 tattggcccc gccgtctatg ccctgggcgc gctgatgctg accatggtgg cggccgatta
     2881 cgccctggac cggcaggcgg tgtgggaagc gatggagcgg cgcggcctgc atcgcgcgcc
     2941 gccgcgccgc accgggtccg aaggcccgcc cgtgcggctg gggtgcgaca cctgcggcct
     3001 ggtcagccgg gccgaacccg gctccccctg cccgcgttgc gccagtgcgc tgcatcaccg
     3061 caagccgttc agcatcgcgc gcacctgggc gctgggcctc tccgcgctga tcctgtatgt
     3121 gccggccaat tactacccgg tactcacggt gatccggctc ggctggggac agcccagcac
     3181 catcatcggc ggcgtggagg aactcatcgc cgccgggctg tggccgctcg ccgcgctggt
     3241 gttctttgcc agcatcgtcg tgccgggact gaagctgatc gggctgggcg tcctgctgat
     3301 cagcacgcaa cgcggcagcc gccggcggtt gcgtgaccgg acaaggctct accgcatcgt
     3361 cgattccatc ggccgctggt cgatgatcga catcttcatg ggatcgatcc tggtggcgct
     3421 gctgcagttc ggcatcatcg cgcgggtcac gccggggttc ggcgcggtcg ccttcgccgc
     3481 cgtggtcgtg ctgaccatgc tggcggcgca aagcttcgat ccgcgcctga tgtgggacgc
     3541 ggccggcgta caggcggagt atcaggagtg agcgaccagc cgcactcgcc tggaccgccg
     3601 ccacacgcct cggtgcggtc caaggtcgat cggcgctggc gcctgtcgct gatctgggcg
     3661 attccgatcg tcaccctcgc gcttgggctc tggctggcct ggcacaccat ggagagccgc
     3721 ggcccgatca tccacatcag cttcgaaagc gccgagggtc tggtggccgg ccagtcgcac
     3781 gtcaagcaca aggacgtgga catgggcgtg gtgcagaaca tcgcgctgag caagaacctg
     3841 cagcacgtcg tggtcacggt gcggatgacg cgggaagcag agccgctgct gaccgaccgg
     3901 acgaagttct gggtggtgaa gccgcgcttc ttcgccggcg cgctcagcgg gctggagacg
     3961 ctggtgtcag gcagctacat cgagatgctg ccgtcaccca agcccggcga gcggacccgc
     4021 aacttcaccg gcctggaaac cccgccggtg ctgcaatcgg atgtgcccgg caagaccttc
     4081 ctgctgcatg caccgcggct ggggtcgatc agcctcggct ccccggtgtt ctaccgggac
     4141 ctcgatgtgg gtgaagtgct gggctgggat atcggcgaca tggcgagcac cgtgacggta
     4201 cacgcgttcg tccgcgcccc ctatgacaaa tacgtccacg acaactcgcg attctggaat
     4261 gcctccggcg caaagatttc actcggcggg aacggcgtgc aactgcaact ggaatcgctg
     4321 cgcgcggtgg tgttgggcgg gattgcgttc gagacgccgg atcagggcaa gggctctccc
     4381 gtcacgcgga acctgcatga attcccgctg tatccggaca agcaggcggc ggattccgcg
     4441 aacttcgcgc agaggatctc ctgcatcacc tacctgaccg gctcggtggc cggcctcgca
     4501 cccggggcat cggtgacgct gcacggcctg ccggtcggac aggtcgagtc cgtgatgctg
     4561 aaatatgaca agaagcacga caccgtggtg gtcccggtcc ggttcgacct ggaacccggg
     4621 cgcgtctccg gcctgacgct gccaagcggc agcgggctgg atgcggagat gcgggatgtg
     4681 gtgcggcgcg gcctgcgggt cagcctggag tcgaccaacc tgatcaccgg ccagaagcag
     4741 cttgccatcg cgctgcatcc cgacgcgccg cctgcgacgc tggagaagca ggacggcgcc
     4801 tatgttatcc cggttgctcc cggcggttcc ggcggcgacc tgtccggctc ggcgaccgag
     4861 ttgctggcga agctgaacgc gatccccttc ggccagatcg gccacaacct gaaccagttg
     4921 atcgccggtg cgaacggcct ggtgaacaac agcgggctgc agaaggcggt cgggtcgctg
     4981 caaggcacga tgggcgagct gcagggcctg gtgcatcggc tgaataccgc cagcgggcca
     5041 ctggtgaagg atctgcccgg catggccagg caactggacg gggcagtgaa gcagctcaac
     5101 ggtctcgccg cctcgatgca gcagggctat ggcagcaatt ccgacctgca caatcagctc
     5161 tcccggctgc tggcgcagtt gaacgacacc gcgcagtcct tccgggcctt cgccgatctg
     5221 ttgcagcgcc atccggaagc gctgatccgc ggccgcaaat aaccaaggaa ccggccatgc
     5281 ccctgacccg ccgtcatgcc ctggtcctgg ccgccctgct gccggccgcc tgcgcctcac
     5341 ccaacccggc gctgttcaca ctggccgcgg agccgggaca gacgcagggc aacgggccga
     5401 agctggtcaa ggtgcgtgag gtgtcgttgc cgcactacat ggagcgctcc gagatcgtgc
     5461 gttccaccga gaactaccag ctggtcgtgc tgccgaacga ctggtggggc gaatcgctct
     5521 cctccatgat gaaccgcgtg ctggtgcagg acctgtcgca gcgcctgccg gacagcacgg
     5581 tggtgtcgga gatcggcgcg atttccggca cgcccaatgc cgcggtggcc atcaacgttc
     5641 tgcgcttcga cgcggaccgg tccggcgcgg tggtgctgga tgccgagatc gccgtcaacg
     5701 ggcatccgca cggcaatcgc cgggtgcacc tgacggtgcc actggccggg cccggcacgc
     5761 cggccctggt cagcgcgatg agtgcggcga tcggcaagct ggccgacatc gtcgcccgca
     5821 tgctggcctg acggccgaat tcctccgcct ggccggtacg cacaaaccga gtcgccattt
     5881 gccgccgcgt gtactaggct cgctccaaca atcataacga tccaggaggg aggcatgtac
     5941 caaccgccga aagtcccgat tcctccgtgt catgcgcggg cttggcccgt gcacccacct
     6001 cttgctgtgg cgcacgcagc agagacgtgg gtggccgggc caagcccggc catgacacgg
     6061 gggaggagac gctccgccga agggtcagaa cgatgggccg ctggcgtgat accggcccgc
     6121 gaaaggcccg cctctgggga tggcagactt cttcgtggcg ggcctgcgcc cgccacgacg
     6181 gtggttgcgc gccgggtgtg tcacgcttcg tgcgggttgg tatgagcgct ggccagtacg
     6241 cactctcatt ggagagtgcc gtcgcggatg aacgtccgcg gcgacgcgcc tggcatcgcc
     6301 atctgcacgg ttcggatctc gcctggtcga tcgccttcgt gcttccctac gcggcggtct
     6361 tcgtgggatt cgtcatctat ccgatactct atgcgctgtg gctgggacgc gatccggcgc
     6421 tgtactccct gctgctgacc aatccaaagt tcctggaagc agcggtcaat accgcggtgt
     6481 tcgtcggtgt cgcggtcaat gcgcagatgt ttctggcctt gctgctgtcc ggattctttg
     6541 cgcgccgccg ccgctggatc aagatgctgc tggtcctgtt catgctgccc tggaccttgc
     6601 cggcagtgcc ggcctacctg tcgttccatt ggatgctggt cacgggccac ctgggcctga
     6661 tcgaccacct gctgcaagtg ctgttcggca tcgaagggcc caactggttc atccacggct
     6721 ggctcggcct cacctgcaac atggtcgcgt cggtttggaa gtggctgcca ctgtggacgc
     6781 tgatcctgct cggcggccgc atggcaatcc cgcaggatct gtacgatgcg gcggatatcg
     6841 acggcgcgtc tttgtatcac cgctttgtcc atatcaccgt gccgctgctg gccaacctgt
     6901 acctgatcag cacattgatc tccgccatct ggaccttcgg cgacgatacg ccggtgctgt
     6961 tcgtgtccgg tggcggcccg gccttcgcgt ccagcgtggt ctccctgctc agcttccatt
     7021 atgccttcga ccttgcccag ccgacgctcg gtgtcgctgc cggactttgc atgttgccgc
     7081 tgttgatccc gctgatcgtg ctgctgaccc gtgcattgca ccgtaccgag gtgcagttgt
     7141 gagcgctgtc gtggcagaga agcgaacccg gcgccgcagg aactaccggc tccggcggac
     7201 gctggtcgag ggcagttcca tcctgctggg tgcggcgatc gtcatctggt cgctgattcc
     7261 cgtgtacaac atgttcctcg ttgcgctcga tccgctcggt cataacgagt tctccggcct
     7321 gctctggccg gaacacccga cactggaggt gttcgccaat ctgtggaccg aaggcgccga
     7381 cgacgtggag gggatctggc ggcaattcgg caacagcatc tatatcggcg cggtcgccac
     7441 ggcattgacc gtgctgatca gctcgatggc cagcttcgcg atcgggcgca tgcggctggg
     7501 ccggcaatgg accattaccg gcacggcact gctcacctac gcgattccgg cctcggtgct
     7561 gatcatcccc ttcatccgga tcatgcagat ctacgggctg tcggataatc tctgggcggt
     7621 gatcgcggcg gagatcacct tcgccacgcc gtacgcgatc ctggtgctgc tgtattacgc
     7681 caagctgatc ccggcagagc tggacgacgc ggcacgcatc gacggggcct ccgtggtgca
     7741 gatctatttc cgtatctacc tgccgctgat ggcaccggcg ctggccgcgg tcgggaccta
     7801 cgcgctgctg tacgcgtgga acgactattt ctaccagtac atgctgctga tgtcggagaa
     7861 gcacaccacg gtcgccgtgg cgctggagca gttcttcgac gacgacgatg caccatggaa
     7921 ctacatgatg gcgatcgcca tcatctattc cctgccgccg atcgccacct tctatgcgct
     7981 acgccgcttc atggcgaccg gactgaccgc gggcgggatg aaaggatagc gcacccgtcg
     8041 ggctacatcg tcatggcggg cgaaggcccc atagacgtta ggatagggga tgacgagcgg
     8101 ccaaacccgg tgtcatggcc gggctcggcc cggccacgca cg
//
LOCUS       DASSZH010000044         2836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_478229, whole genome shotgun sequence.
ACCESSION   DASSZH010000044 DASSZH010000000
VERSION     DASSZH010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478229"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..1204
                     /locus_tag="VFN42_00835"
     CDS             65..1204
                     /locus_tag="VFN42_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010507068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HET8995188.1"
                     /translation="MSDDLPNPALLPAGLRDLLPPEAETEASAVEALMHVFATHGYQR
                     VKPPLLEFEDSLLAGSGAAVAEQTFRLMDPDSQRMMGLRADTTPQVARIATTRLANAP
                     RPLRLSYAGQCLRVRGNQLAPDRQVAQAGIELIGPDSPAADAEIVLVGAEALQAVGLT
                     RVSFDLTLPMLVPTLLDEAAITGPSRASLGRALDRKDAASVARHGGVLAPVLTELLLA
                     AGPADRALAALKAAHLPSSAGALAARLTAAVAAIRAQAPALRLTVDPVEFRGFRYETG
                     VSVTIYAPGRHEELGRGGRYLCGETEPATGLTLLPDAVLRAAPARQVRPRVYLPLGTP
                     QDAAARLRREGFATVAGLEAVTDDAAEARRLGCSHLLRDGQPTAA"
     gene            complement(1627..2292)
                     /locus_tag="VFN42_00840"
     CDS             complement(1627..2292)
                     /locus_tag="VFN42_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HET8995189.1"
                     /translation="MTDEVEIEGGIRDKLIARKQDWAREGRLLTGTTADPARDRLPPG
                     QRLVRDWPVLDLGAEPNVTPEKFRLDLDGAVENRLSLTLDAFMALPQVDSVSDIHCVT
                     QWSRYDNRWKGVAARTILERVLPQPEVKHVVFHAYDGYTTNIRLDQFDQPDVFLVHEW
                     EGKPINRAHGGPVRMLVPRLYLWKSAKWLRRIQFTISDHPGFWEQRGYHNNADPWLEE
                     RYG"
     gene            complement(2429..>2836)
                     /locus_tag="VFN42_00845"
     CDS             complement(2429..>2836)
                     /locus_tag="VFN42_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995190.1"
                     /translation="DPRFAGGSLLLVNRNFGCGSSREHAPQALRRFGIAAIVGESFGE
                     IFAGNCVAIGMPCVQLGAEAMARIQALNEREPQTRFTLDLAALTLSAGGETFPVTLAE
                     GRRRQFVEGSWDSTAVLLQAGDAIEHTLAGLAY"
BASE COUNT          412 a         1048 c          940 g          436 t
ORIGIN      
        1 ggttggaacc gccgggaacc ccgccccttc cccccgccgc aatgacgcgg ctaagaaccg
       61 cgacatgagc gacgatctgc ccaacccggc cttgctgccg gccggcctgc gcgacctgct
      121 gccgccggag gcggagacgg aagcctccgc cgtcgaggcg ctgatgcatg tcttcgccac
      181 gcatggctac cagcgggtga agccgccgct gctggagttc gaggactcgc tgctggccgg
      241 ctccggtgcc gcggtggcgg agcagacgtt tcgcctcatg gacccagaca gccagcgaat
      301 gatggggctg cgcgccgaca ccacgccgca ggtggcgcgc attgccacca cccggctggc
      361 caacgcaccg cgcccgctgc ggctgtccta tgccggccag tgcctgcggg tgcgcggcaa
      421 ccagctggcg ccggaccggc aggtggcgca ggccggcatc gaactgatcg gccccgacag
      481 cccggcggcg gacgcggaaa tcgtgctggt cggcgccgag gcgctgcagg cggtcgggct
      541 gacccgggtc agcttcgacc tgacgctgcc catgctggtt cccaccctgc tggacgaagc
      601 cgccatcacc gggccgtcac gtgcctcgct cgggcgggcg ctggaccgca aggacgcggc
      661 ctcggtggct cgccatggcg gcgtgctggc gccggtgctg accgagctgc tgctcgcggc
      721 cggccccgcc gaccgcgccc tggcggcgct gaaggcggcc cacttgccca gttccgccgg
      781 cgcgctcgcc gcccgcctga ccgcggcagt ggccgcgatc cgcgcccagg ccccggcgct
      841 gcggctgacc gtcgatccgg tggagttccg cggcttccgc tacgaaaccg gcgtatcggt
      901 gacgatctac gcccccggac ggcacgagga actcggccgc ggcgggcgct atctatgtgg
      961 tgagacggag cccgccaccg gcctgaccct gctgcccgac gccgtgctgc gcgcggcccc
     1021 ggcgcgccag gtccggccgc gcgtctatct gccgctgggc accccccagg atgctgccgc
     1081 gcgcctgcgc cgcgaaggtt tcgccaccgt cgccggcctg gaggccgtca cggacgacgc
     1141 agcggaggcc cggcggctcg gctgcagcca tctgctgcgc gacggccagc cgacggcagc
     1201 ctgagggttc tgtcgcattg ccctttcctg ccaagctctg cgtctttggc atgccagata
     1261 ccgcatacaa cattgcaaag aatcgcttgc atacgaacga ccaagggcct agcttccgtc
     1321 tcgcagcaac gcggtcactg accgtgctgt ctgctttctt ggacgtttcc tccctaaact
     1381 tggcggcgcc gcaaagcgcc gccatttttt ttgcgctgcc gccgcgcacc ggcttgttgc
     1441 gggaaagccg gtcccggcgt tcatgccgct acggaaccgt gatccggcgg aaggatctgg
     1501 ggctatggac aacagcccta ccggatggta ggttgcgctc acgatcagtt ggaagccaag
     1561 tttagggccc gaggaaacga cacaccccgc cagcgcaacc gcggcggggt gtttgttgtc
     1621 cggcgctcag ccgtagcgct cctccagcca cgggtcggcg ttgttgtggt agccgcgctg
     1681 ctcccagaac ccgggatgat cgctgatggt gaactggatc cgccgcagcc acttcgccga
     1741 tttccacaga tacagccgcg gcaccagcat ccgcaccggc ccgccatgcg cgcggttgat
     1801 cggcttgccc tcccactcat gcaccaggaa gacgtccggc tgatcgaact ggtccagccg
     1861 gatgttcgtc gtgtagccgt cgtaggcgtg gaacacgacg tgcttcacct ccggctgcgg
     1921 cagcacccgc tccaggatgg tgcgcgccgc cacgcccttc caccggttgt catagcgcga
     1981 ccactgggtg acgcagtgga tgtcggacac gctgtccacc tgcggcagcg ccatgaacgc
     2041 gtccagcgtc aggctgagcc ggttttccac cgcgccgtcg agatccaggc ggaacttctc
     2101 cggcgtgacg ttcggctccg cgcccaggtc cagcaccggc cagtcgcgca ccagccgctg
     2161 gcccggcggc agcctgtcgc gcgccggatc ggcggtggtg ccggtcagca ggcgcccttc
     2221 ccgcgcccag tcctgcttgc gcgcgatcag cttgtcgcgg atcccgccct cgatctccac
     2281 ctcgtcagtc atcctcgtcc ttcccgatct gccgctgcag agactcgcgc gccccgcgcg
     2341 cgttacgcca cacggtgtat cgcacaagcc tggcagatgg gcgctggccg gccgcgttga
     2401 agtctggagg caccgaccgg ggcagacgtc agtatgccag cccggccagg gtgtgctcga
     2461 tcgcgtcgcc ggcctgcagc agcacagcgg ttgaatccca ggagccttcg acgaactgcc
     2521 gccggcgtcc ttccgccagc gtgaccggaa acgtctcgcc ccccgccgac agcgtcagcg
     2581 ccgccaggtc gagggtgaaa cgggtctgcg gctcgcgctc gttcaacgcc tggatccgcg
     2641 ccattgcctc ggcgccgagc tggacacacg gcatgccgat ggcgacgcag ttgccggcga
     2701 agatctcgcc gaacgattcg ccgacgatcg ccgcgatgcc gaaccgccgc agcgcctgcg
     2761 gggcgtgctc ccgcgacgaa ccacagccga aattgcggtt caccagcaac aggctgccgc
     2821 cggcaaagcg cggatc
//
LOCUS       DASSZH010000045         4341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1023866, whole genome shotgun sequence.
ACCESSION   DASSZH010000045 DASSZH010000000
VERSION     DASSZH010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023866"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VFN42_00850"
     CDS             <1..486
                     /locus_tag="VFN42_00850"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HET8995191.1"
                     /translation="RPGSLIVPPLRLWRIVTGRHPAWSGEGARLFGQRWNPPGYSAIY
                     TGTSFAVCLVEVLVHANRTAPPSAARYVEAEVPADVSRELFNPGLHHGWDDPLNPSVA
                     QSFGRRWIAERRSALLVVPSVVTGGRDTNVVVNPDHPDASRISVGPERPVTFDSRLFR
                     R"
     gene            complement(497..1801)
                     /locus_tag="VFN42_00855"
     CDS             complement(497..1801)
                     /locus_tag="VFN42_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995192.1"
                     /translation="MLADRYDLPLSTTSAAAADAYVQGAELALTLYPGALEAFDQAIA
                     ADPGFALAHAGKAQVLIRYGDAAAGRAALAAAKDVAGGLPAREASHVTFFDRVLAGQT
                     DAAISLMYEHLAAWPRDALVVATAGNPNGLLGGSGRIGQKHQIAVLMDELAPQYGDDP
                     WFLAYHAMALSEDGQLAAARPLIERSVTANRHNAHGAHGFAHVCYESGESDTARGWLA
                     SWLAEYPRDAFFHGHLSWHLSLCELQAGNWDNALRLYREAIVPERHSGGPQQKMSDGA
                     AFLWRSELAGHPRDDAAWRRLYAYAGEALPRPGNGLADLHVILAQAVMRDDAGLAART
                     RQMEEAASAGRYPSGSYLPAMARGLVAFERGDMAAAIEALAPLAGENERIGGSRAQHD
                     LIAFTLLKAYLDAGRLEEARGLLATRRPGASGVPVAGVAAVH"
     gene            2170..2949
                     /locus_tag="VFN42_00860"
     CDS             2170..2949
                     /locus_tag="VFN42_00860"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET8995193.1"
                     /translation="MPEYENILVETHGRVGLIRLNRPKALNALCDALMQELGDQLLAF
                     DADKAIGAIVLTGNERAFAAGADIKEMRSRTHPEALTEDFIGKRWETVLQVKKPVIAA
                     VAGFALGGGCELAMMCDFILAADTAKFGQPEINLGVLPGAGGTQRLTRAVGKSKAMDM
                     VLTARMMDAAEAERANLVSRVFPAEQLLPEALKIAGQIANLSPVAVQLAKQSVNRAFE
                     TTLVEGVRYERAVFLSLFGTPDQQEGMAAFVDKRKPNFRLG"
     gene            3063..4238
                     /locus_tag="VFN42_00865"
     CDS             3063..4238
                     /locus_tag="VFN42_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET8995194.1"
                     /translation="MQNTAVIDGRGSAPDLHPPRLLLVSSSPRWRNAVQAAARDIHGA
                     RVAMCAAREAIVRLAGTADRYSHVLVQPGSADGWLAELVDLSAASGASGTELLLLAPP
                     EPPPPRVGVIRSASRRSVRDALVPSRIAAAANEPGMHPSELLDALSGAMIETRYQPIV
                     RMSDRQPIALEALARLKHPTRGTLPPDYFVPQLENAGLAAELTDRVGSRAFADMAGPV
                     GALGLLLSLNFPLDVLLVPEALARLDARRREARIISDRVVIELTESRPVDDIVALGRA
                     VERLRLGGYRVSIDDVSPDAPRVAELLTLPFTSVKLDKQVVQGADQPENARFIDSVVR
                     TAGRTSMLVIAEGVEDEPIWERIQALGADYAQGFLVARPLPATAVTVWHEAWTQAGL"
BASE COUNT          659 a         1542 c         1462 g          678 t
ORIGIN      
        1 cggcctggct ctctgatcgt cccgccactc cggctttggc gcatcgtcac cggcaggcat
       61 ccggcctggt ccggagaggg agcccgcctt ttcggtcagc gatggaaccc gccgggatac
      121 tcggccatct acaccggcac gagcttcgct gtgtgtctcg tcgaggtact tgtccacgcc
      181 aaccgaacag cgcccccaag cgctgcgcgc tacgttgagg ccgaagttcc cgccgacgta
      241 tcacgcgaac tgttcaaccc cggcttgcat catggctggg acgatccgct caatccctcg
      301 gtcgcccaga gcttcggcag gcgctggatc gcggaacgcc gatcagccct cctggtcgtc
      361 ccctcggtgg tgacgggcgg gcgcgacacg aatgtcgtgg tgaatccgga tcacccggat
      421 gcctcccgga tctcagtggg acctgagcgg cccgtcacct tcgactcgcg cctgtttcgg
      481 cgttgagcct tccgcctcaa tgcaccgccg cgaccccggc caccggcact ccggacgccc
      541 ccggccgtcg cgtggccagc agcccgcgcg cctcctccag ccgtcccgca tcgagatacg
      601 ccttcaacag cgtgaacgcg atcaggtcgt gctgcgcccg gctgccgccg atgcgttcgt
      661 tctccccggc cagcggcgcc agcgcctcga tcgccgctgc catgtccccg cgttcgaacg
      721 ccaccaggcc gcgcgccatt gcgggcaggt aggaacccga ggggtagcgc ccggcgcttg
      781 ccgcctcctc catctgccgc gtgcgcgcgg caagcccggc atcatcgcgc atcaccgcct
      841 gcgccaggat cacgtgcagg tcggcgagcc cgttgcccgg ccgcggcagc gcttccccgg
      901 cataggcata cagccggcgc cacgcggcat cgtcgcgcgg atgcccggcc agctcggagc
      961 gccacaggaa cgccgcgccg tccgacatct tctgctgcgg gccgccgctg tgccgctccg
     1021 gcacgatcgc ttcgcggtac agccgcagcg cgttatccca attaccggcc tgcagctcgc
     1081 acagcgacag gtgccagctc agatgcccgt ggaagaacgc atcgcgcgga tactcggcca
     1141 gccaggaggc cagccagccg cgcgcggtgt ccgattcgcc gctctcgtag cagacatgcg
     1201 cgaaaccgtg cgcgccgtgg gcgttgtgcc ggttcgcggt caccgaccgc tcgatcagcg
     1261 gccgagcggc ggcaagctgg ccgtcctcgg acagcgccat ggcgtgatag gcgaggaacc
     1321 agggatcgtc gccgtactgc ggcgccagct catccatcag cacggcgatc tggtgcttct
     1381 gcccgatgcg gccggagccg ccgagcaggc cgttgggatt gccggcggtg gccaccacga
     1441 gagcgtcgcg cggccatgcc gccaggtgtt catacatcag gctgatcgcg gcgtcggtct
     1501 gccccgccag cacccggtcg aagaacgtga cgtggctggc ttcccgcgcc ggcaggccgc
     1561 cggccacatc cttggccgcc gccagagccg cgcgccccgc cgcggcatca ccatagcgga
     1621 tcagcacctg cgccttgccg gcatgcgcca gggcaaagcc gggatcggcg gcgatggcct
     1681 ggtcgaaggc ttccagcgcg cccgggtaga gcgtcagcgc cagctccgcg ccctgcacgt
     1741 aggcgtccgc tgctgccgcg gaggtggtgg acaagggaag gtcgtagcgg tccgcgagca
     1801 tgatggactc ctcctgcggg tctttcgtgg aagtctatcc ttctctccgg ccatcacaag
     1861 aaagactctg cccgcagccg cctctgcccc gcatacgcgc taccataagc cccgcacagg
     1921 cgcgatttcc gcgtcgtggc cgggcttgac ccggccaccc gcccggaaat gatcgggtcg
     1981 ggttgcaacg gaacacgcct tgtcgggagt gggtggccgg gtcaggcccg gccacgacac
     2041 gatgaagcgc cgcacgacct tatgttcgcg cctatgctct gcccccggcg tttcgccgct
     2101 cggcttgcct ccgcggcggg cccggcgcaa ccatgctgca ccgcgctcat cgccgcagga
     2161 ggcccgccaa tgcccgaata tgaaaacatc ctggtcgaaa cgcatggccg agtcggcctg
     2221 atccggctga accggccgaa ggcgctgaac gcgctgtgcg acgcgctgat gcaggaactc
     2281 ggcgatcagc tcctggcctt cgatgcggac aaggcgatcg gcgccatcgt gctgaccggc
     2341 aacgagcggg cctttgccgc cggtgccgat atcaaggaaa tgcgttcccg cacccatccc
     2401 gaggcgctga ccgaggactt catcggcaag cgctgggaaa cggtgttgca ggtgaagaag
     2461 ccggtaatcg cggcggtggc cggcttcgct ttgggcggcg gttgcgagct ggcgatgatg
     2521 tgcgacttca tcctggcggc cgacaccgcg aagttcggcc agccggagat caacctgggc
     2581 gtgttgccgg gcgccggcgg cacccagcgg ctgacccggg cggtgggcaa gtccaaggcg
     2641 atggacatgg tcctgactgc ccgcatgatg gacgcggcgg aagcggagcg cgccaacctg
     2701 gtttcacggg tgtttccggc cgagcaattg ctgccggaag cgctgaagat cgccgggcag
     2761 attgccaacc tgtcgcccgt cgcggtccag ttggccaagc aatcggtgaa ccgggccttc
     2821 gaaaccacgt tggtggaggg cgtgcgctac gagcgggcgg tgttcctgtc gctgttcggc
     2881 acccccgacc agcaggaggg catggccgcc ttcgtggaca agcgcaaacc aaatttccgg
     2941 ctgggctgat taacgctacg tttacgttgg actaacaggc cgggggcggt atagggtgcg
     3001 cgttgcaaac ctccatcctc gccgcaggtt ggaccataaa gggaagaaca gtcgtcccgg
     3061 ccatgcagaa caccgccgtc atcgatgggc gcggcagcgc gcctgacctg catccgccgc
     3121 ggctgctgct cgtcagctcc agcccacgct ggagaaatgc ggtccaggcc gcagcccggg
     3181 acatccatgg cgcccgggtg gccatgtgtg ccgcacgcga ggcgatcgtc cgcctggccg
     3241 gcacagccga ccgctattcg cacgtgctgg tgcagcccgg cagtgccgat ggctggctgg
     3301 ccgagctggt cgatctcagt gccgcttcgg gcgcgtccgg aaccgaattg cttctgctcg
     3361 cgccgcccga accgccgcca ccccgcgtcg gcgtcatccg ctcggcgagc cgccgctccg
     3421 tgcgcgatgc cctggtcccg tcccggatcg ccgcagcggc gaacgagccg ggcatgcacc
     3481 cgagcgaact gctcgacgcc ctgtccggcg ccatgatcga gacccgctat cagcccatcg
     3541 tgcggatgtc cgaccggcag ccgatcgcgc tggaagcgct cgcccggctg aagcacccga
     3601 cccgcggcac cttgccgccg gattatttcg tgccgcagct cgaaaacgcc ggactggcgg
     3661 cggaactcac cgatcgggtc ggctcccggg cgttcgccga catggcggga ccggtcggcg
     3721 cgctgggcct gctgctgtcg ctgaattttc cgctcgacgt gctgctggtg ccggaagcgc
     3781 tggcccgtct ggacgcgcgc cggcgggagg cccgcatcat ctcggatcgt gtggtgatcg
     3841 agctgaccga gagccggccg gtggacgaca ttgtcgcact gggacgcgcg gtggaacgcc
     3901 tgcgtctcgg cggctaccgc gtatcgatcg acgacgtctc gccggatgcg ccgcgggtgg
     3961 ccgaattgct gacgctgcct ttcaccagcg tgaagctgga caagcaggtg gtgcagggcg
     4021 cggatcagcc ggagaatgcg cgtttcatcg actctgtggt gcggaccgcc ggccggacat
     4081 cgatgctcgt gattgccgag ggcgtcgaag acgagccgat ctgggagcgc atccaggcgc
     4141 tgggcgccga ttacgcgcaa gggttcctgg tcgcccgtcc gctgcccgcc accgcggtga
     4201 cggtgtggca cgaagcctgg acgcaagccg ggctctgacg cacgcacgcc gcgcagggac
     4261 cttggccggc cggctcggcg aaggctaaac ccacggcatg gcaggatccg cccgtattcc
     4321 gacgcccgac ggcgcctccc c
//
LOCUS       DASSZH010000046         2678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_205687, whole genome shotgun sequence.
ACCESSION   DASSZH010000046 DASSZH010000000
VERSION     DASSZH010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205687"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..496
                     /locus_tag="VFN42_00870"
     CDS             110..496
                     /locus_tag="VFN42_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995195.1"
                     /translation="MQRKIVLLAAALCGMAGAMQAHAQNAPAMVSQTAKGKVLTDAHG
                     MTLYVFDKDMAGKSMCNGPCIHNWPALHATAMDKPSGDWTIITRDDGAMQWAYKGKPL
                     YTWIKDKHAGDMTGDGVNHVWHVATP"
     gene            complement(827..1384)
                     /locus_tag="VFN42_00875"
     CDS             complement(827..1384)
                     /locus_tag="VFN42_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995196.1"
                     /translation="MLLNMGVSLRLLDEHAACSNGLQTCNAPSARVALRRRSAANRCV
                     IAAWWGNQFPQVGKTVVQCTPAVCKARFPRRGSAPSGDVRPDAQFGFFQPARRTVSAN
                     KWSLEMRKTMFLVAAAMLSLGSASAFAAGSASVNQQVPNQVAAYQAPQAQFATTNQAT
                     APESQDGTTVILQSNPSAPLQTLGG"
     gene            1371..2534
                     /locus_tag="VFN42_00880"
     CDS             1371..2534
                     /locus_tag="VFN42_00880"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HET8995197.1"
                     /translation="MLSSTDRILVSHVGSLPRADSLSDLLIRQEAGEAIDTAALAREV
                     DAATAHVIDKQVEAGVDVGNDGEQSRVAFQTYVPRCMCGFGGESKRPPARDQQQFPSW
                     VRQAAARFPHSARTINAAAAVAEVRYVNSAPIREDAARLKRLGGGFRECFMTAPSPGI
                     VATTMLNQHYDSHEAYVMALAAALSNEYRAIHDAGLILQIDAPDLAMERHRFFDHLSE
                     ADFLRQLEMHVAAINRGIDGIPRERIRLHVCWGNNDGPHIYDIAMATILPELYRANVG
                     ALSIEFANPRHQHEYAALRDAPLPPHMLLMPGVLDTTTNFVEHPEVVARRLEEAVAAV
                     GDRERVIAGTDCGFGTFAGREYVAEEIVWVKLAAAAEGARIASRRLWGRAAGD"
     gene            complement(2531..>2678)
                     /locus_tag="VFN42_00885"
     CDS             complement(2531..>2678)
                     /locus_tag="VFN42_00885"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET8995198.1"
                     /translation="DVHLGTWHWFVFNVADAAIVCGVAALVLESQWGRKGSAPRRQAE
                     DNGA"
BASE COUNT          477 a          857 c          907 g          437 t
ORIGIN      
        1 cgctgacggg aacataattg ccgacaggcg tggaatatcc cgccgggcag ttcgtcaacc
       61 ctgcgtgcgg tccgcgtgtg gccggcaagt acgggaggga tacggaaaga tgcagcgaaa
      121 aatcgtgttg ctggcagcgg cgctgtgcgg catggccggc gcgatgcaag cccatgcgca
      181 gaacgccccc gcgatggtct cacaaacggc gaagggcaag gtgctcaccg acgcgcatgg
      241 catgaccttg tacgtgttcg acaaggacat ggcgggaaaa tcgatgtgca acgggccgtg
      301 catccataac tggccggcgc tgcatgccac ggcgatggac aagccgtcgg gcgactggac
      361 catcatcacg cgcgacgatg gcgcgatgca gtgggcgtac aagggcaagc cgctgtacac
      421 ctggatcaag gacaagcacg ctggcgacat gacgggagac ggggtgaacc acgtctggca
      481 cgtcgcaacg ccctgacggt cggggcacag cgtcctgggg gaaggtccgc ctgtcgcgac
      541 tggctatggg agactccgaa agcaggcttg cccttgtgaa tgcccccaac caatcccggg
      601 tgtcaggccg ttgccacgac gcggtaccgc gctgaactga tggccggcgc aatgcaccag
      661 caaaagcccc ccggcaccag gtgatgccga ggggcttttg aaggtgccgc gatcaagcgt
      721 ggccagggtc agccctggtt caccacctgg aagcatccgc cgtggcgaag cgcgtcacgg
      781 catcgccgct caggccatcg atcgtgtggc cgcgggtttt gtccgctcag ccgccaagcg
      841 tctgcagcgg ggcggacggg ttggactgca agatcaccgt ggtgccatcc tgcgactcag
      901 gcgccgtcgc ctggttggtg gtcgcaaatt gggcctgcgg tgcctggtag gccgcgacct
      961 ggttcgggac ctgctggttg acgctggccg atccggcggc aaatgcactg gcagagccaa
     1021 ggctcagcat cgcagccgcg acaaggaaca tcgtctttct catctcgaga ctccacttgt
     1081 ttgcagaaac cgttcgtcgt gccggttgaa agaaaccgaa ctgagcgtcc ggccgcacgt
     1141 ctccagatgg agcgcttccc cgccgcggaa atcgcgcttt gcacacggca ggtgtgcact
     1201 gcacaacggt tttgccgact tgcgggaatt ggtttcccca ccatgcggcg atcacgcacc
     1261 ggttcgccgc agaccggcgc cgcagcgcaa cgcgtgcgct cggcgcattg caggtttgca
     1321 gcccgttgct gcatgctgcg tgttcgtcca acaaacgcag ggaaacgccc atgttgagca
     1381 gcaccgaccg catcctggtc agccatgtcg gcagcctgcc gcgggcggat tccttgtccg
     1441 acctgctgat ccgccaggaa gccggcgagg cgatcgacac cgcggcgctg gcccgtgagg
     1501 tggacgccgc aacggcgcat gtgatcgaca agcaggtgga agccggtgtc gacgtgggca
     1561 atgacggcga acagtcgcgc gtcgccttcc agacctatgt gccacgctgc atgtgcggct
     1621 ttggcggcga gtccaagcgt ccgccggcgc gcgatcagca gcaattcccc agctgggtgc
     1681 gccaggcggc ggcgcggttt ccgcactcgg cgcggaccat caacgccgcg gccgcggtgg
     1741 cggaagtgcg ctacgtcaac agcgcgccga tcagggaaga tgctgccagg ctcaagcggc
     1801 tcggcggcgg cttccgcgaa tgtttcatga ccgcgccgtc acccggcatc gtcgccacga
     1861 cgatgctcaa ccagcattac gacagccacg aagcctatgt gatggcgctg gcggcggcat
     1921 tgtcgaacga gtatcgcgcc attcacgatg cggggctgat cctgcagatc gacgcaccgg
     1981 acctggcgat ggagcggcac cggttcttcg accatttgtc cgaagcggac ttcctgcggc
     2041 agttggaaat gcatgtggcg gcgatcaatc gaggcatcga cggcattccc cgcgagcgga
     2101 tccggctgca tgtctgctgg ggcaacaatg acgggccgca tatctacgac attgccatgg
     2161 cgaccatcct gccggagctg taccgcgcca atgtcggcgc gctgtccatc gagttcgcca
     2221 atccgcggca ccagcatgaa tacgccgccc tgcgcgacgc tccgctgccg ccgcatatgc
     2281 tgctgatgcc cggcgtgctg gataccacca ccaatttcgt cgagcatccg gaagtcgtcg
     2341 cgcgccggtt ggaggaagcc gtcgccgcgg tgggtgaccg ggagcgggtg atcgccggaa
     2401 cggattgcgg cttcggcacc ttcgccgggc gcgaatatgt ggcggaggag atcgtctggg
     2461 tgaagcttgc cgcggcggcc gagggggcgc gcatcgcctc ccgccggcta tggggccgcg
     2521 cggccgggga ctaggccccg ttatcctccg cttggcgccg cggagcgctg cccttgcggc
     2581 cccattggct ctccagcacc agcgccgcca cgccgcagac gatggccgca tcggcgacgt
     2641 tgaacacgaa ccagtgccag gtgccgaggt ggacgtcg
//
LOCUS       DASSZH010000047         2508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1194274, whole genome shotgun sequence.
ACCESSION   DASSZH010000047 DASSZH010000000
VERSION     DASSZH010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194274"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VFN42_00890"
     CDS             <1..589
                     /locus_tag="VFN42_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET8995199.1"
                     /translation="MARTFTTVAIAFAASVVVGAAVGALVHAIPRLRRAVDPLLASYY
                     AMPFFVFYPLLVAFFGLNAMPLIAIGFAAAMVAMVISTLNGLDRVPAVLLKVARIHRL
                     GRLRTLLLVVVPAATPSLMTGMKLALAYAFISIIAGEFILSGQGLGYQIAYAYDSFDN
                     RTMYGLMLFVLLVVTMMNMLLHIWEQRLLRRRSRT"
     gene            586..1374
                     /locus_tag="VFN42_00895"
     CDS             586..1374
                     /locus_tag="VFN42_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET8995200.1"
                     /translation="MSATVAQAPSRRTASSAAGRRVLDQLVIILVLLACWQGAYEYAG
                     SVALSSPAETVAYAVAMLGKEWFWESAAATGLAFVYSLLIASVIGVLLGLVLGLWRFA
                     ADVTEPVLAALYTIPKVTLYPLILLLFGLGLSAKVAFGVIHGVIPITLFTLAAVKGLS
                     PVLLRTARAMRLSPSKTILWVLTPAVLPEIFTGLRVGFSLTLLGVLIGEMFASQLGLG
                     FLIVNGINLHNVPLTTAVVLIIVLVAVAANAALLALDRHLRRQR"
     gene            1448..2332
                     /locus_tag="VFN42_00900"
     CDS             1448..2332
                     /locus_tag="VFN42_00900"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HET8995201.1"
                     /translation="MPASHAARLRALMREQRMITAPGAYDAITARLVIQAGFPVVYMT
                     GAGTAATLGFPDYGLITMTEMVDNAARLVRAADAPVIADGDTGYGNELNVVRLVQEHH
                     RAGTAGIHIEDQVSPKKCGHLDDKQIVPREDWLAKIRAAAAARPDPDFLVIARTDSRA
                     VAGFDEAVARCNAALAAGADVAFLEAAQTMEEVAAVPKRVNSPCLYNLVKAGKSPDVP
                     LDDAEAMGYRIVIVPGLLFKTMLHAGDAALAALKAERRHPEVGGLTVRDAFARVGAAE
                     WDAYRTRFRAEQPRAAAE"
     gene            2329..>2508
                     /locus_tag="VFN42_00905"
     CDS             2329..>2508
                     /locus_tag="VFN42_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995202.1"
                     /translation="MTAPLSLFDKIWQRHAVITRPDGAVLLHVGRHLLHDGSRAGFRF
                     LAERKLPLHDALHTYG"
BASE COUNT          359 a          860 c          827 g          462 t
ORIGIN      
        1 catggcgcgc accttcacga ccgtggcgat cgcatttgcc gcatccgtgg tggtgggtgc
       61 cgcggtgggc gccctggtgc atgcgatacc tcgcctgcgc cgggcagtgg atccgctgct
      121 ggcgtcctat tacgccatgc cgtttttcgt cttctacccg ctgctggtcg ccttcttcgg
      181 cttgaacgcc atgccgctga tcgccatcgg cttcgccgcg gccatggtgg cgatggtgat
      241 cagcacgctc aacgggctcg accgcgtgcc ggccgtgctg ctcaaggtcg cccgcatcca
      301 ccgcctggga cgcctgcgca cgctgctgct cgtggtcgtg cccgcggcga cgccgtcctt
      361 gatgacggga atgaagcttg cgctggccta cgccttcatc agcatcatcg ccggcgagtt
      421 catcctgtcg ggccaggggc tcggctatca gatcgcctat gcctatgaca gcttcgacaa
      481 ccgcacgatg tacgggctga tgctgttcgt gctgctggtt gtcaccatga tgaacatgct
      541 gctgcatatc tgggagcagc ggctgctgcg ccgccggagc cgcacatgag cgcgacggtc
      601 gcccaggcgc cgagccgccg gaccgcctcc agcgcggctg ggcggcgcgt gctggatcaa
      661 ctcgtgatca ttcttgtgct gctggcctgc tggcagggcg cctacgaata tgccggcagc
      721 gtggcgcttt cctcgccggc cgagaccgtc gcctatgccg tggcgatgct gggcaaggag
      781 tggttctggg agagcgcggc agcgaccggc ctggcgttcg tttattcctt gctgatcgcc
      841 tcggtgatcg gggtgctgct cggtctggtc ctgggtctgt ggcgcttcgc ggcggatgtg
      901 accgagccgg tgctggccgc gctttatacc atccccaagg tgacgctgta cccgctcatc
      961 ctgttgctgt tcggcctggg cctgtcagcc aaggtggcgt tcggcgtgat ccatggcgtg
     1021 attccgatca cgctgttcac gctggcggcg gtgaaggggc tttccccggt cctgctgcgc
     1081 accgcgcgcg ccatgcgcct gtcaccgtcc aaaaccattc tgtgggtgct cacgcccgcc
     1141 gtgctgccgg aaatcttcac cggcttgcgc gtcggcttct cgctgaccct gctgggcgtg
     1201 ctgatcggcg aaatgttcgc ctcccagctc ggcctgggct tcctgatcgt caacggaatc
     1261 aacctgcaca atgtgccgct gaccaccgcg gtggtgctga tcatcgtgct ggtggcggtc
     1321 gccgcgaacg ctgcactgct cgcactggac cgccacctgc ggcggcagcg ctgacggaag
     1381 ccgcgccgcg gcttcgcctt gagacgcaat tgacgtaagc tcgacgacga cacaagggag
     1441 aatgccaatg ccagccagtc atgcagcacg cctgcgcgcc ctcatgcggg agcagcgcat
     1501 gatcaccgcg cccggcgcct atgacgccat caccgcgcgc ctggtcattc aggccggttt
     1561 tcccgtggtc tacatgaccg gggccggcac ggcggcgacg ctgggcttcc ccgattacgg
     1621 gctgatcacc atgaccgaga tggtggacaa cgccgcccgt ctggtgcgcg ccgcggacgc
     1681 gccggtgatc gccgacggcg acacgggtta cggcaacgaa ctgaacgtgg ttcgcctggt
     1741 gcaggagcac caccgcgccg gcaccgccgg catccatatc gaggaccagg tcagcccgaa
     1801 gaaatgcggc catctggacg acaagcagat cgtcccgcgg gaggactggc tggccaagat
     1861 ccgcgccgcc gccgctgccc gccccgatcc cgacttcctg gtgatcgccc gcaccgacag
     1921 ccgcgccgtg gccggctttg atgaagcggt cgcgcgctgc aacgccgccc tggccgctgg
     1981 cgccgacgtc gccttcctgg aagccgcgca gaccatggag gaggtcgccg cggttcccaa
     2041 gcgggtcaac agcccctgcc tgtacaatct ggtcaaggcc ggcaagagcc cggacgtgcc
     2101 actggatgac gccgaggcaa tgggctaccg catcgtcatc gtgcccggct tgctgttcaa
     2161 gacgatgctc catgccggcg acgccgcgct cgccgcgtta aaggcggagc gccgccatcc
     2221 cgaggtcggc ggcctgaccg tgcgggacgc tttcgccagg gttggcgcgg cggaatggga
     2281 tgcctaccgc acccgcttcc gcgcggaaca gccacgggcc gcggcggaat gaccgcgcca
     2341 ctctcgctgt tcgacaagat ctggcagcgc cacgccgtca ttacccgacc ggacggcgcg
     2401 gtgctgctgc atgtcggacg ccatctgctg cacgacggct cgcgtgcagg ctttcgtttc
     2461 ctggcggagc gcaagttgcc gctgcacgat gctctgcata cctatggt
//
LOCUS       DASSZH010000048         3627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_989661, whole genome shotgun sequence.
ACCESSION   DASSZH010000048 DASSZH010000000
VERSION     DASSZH010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989661"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1922
                     /locus_tag="VFN42_00910"
     CDS             117..1922
                     /locus_tag="VFN42_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET8995203.1"
                     /translation="MPARIEDYALIGDCETAALIGRDGSIDWLCLPRFDAPSCFAALL
                     GGPEHGRWKIAPAGTCRTSRSYRHDTLVLDTVFETDTGACRLTDFMPLRDGNSDLVRI
                     VTGLRGSVDLRMELTIRFDYGRSIPWVRRMDDGSLRAIAGPHMLVLQTRVPLQGQGLS
                     TTSEFTLAAGQTESFTLTYGPSHLRLPMPADPAEVLPATEAFWRDWASHCTVRGPYAD
                     VARRSLITLKALTFDPTGGIVAAATTSLPEFLGGARNWDYRFCWLRDATFTLLALMGA
                     GYYEEAYRWRDWLVRAAAGSPSQIQIMYGLAGERDLTERELPWLPGYANSAPVRIGNA
                     AAEQLQLDVFGEVADALHHARKGELGPSETAWHLQQTLTEHLEKIWAAPDEGLWEVRG
                     PRQCFTHSKVMAWVAFDRAVKAVEDFGLDGPVDRWRALRDHVHEEICRRAFNAELGSF
                     VQSYDSRQLDSSLLLMPLVGFLPPDDPRMIGTVAAIERHLAVDGLVLRYDTAATDDGL
                     APGEGAFLACSFWLADNLVLQGRVPEARERFERLISLANDVGLLAEEYDPRAGRMLGN
                     FPQAFSHVALVNTALNLSRWEGPADQRGQEETARA"
     gene            complement(1948..2391)
                     /locus_tag="VFN42_00915"
     CDS             complement(1948..2391)
                     /locus_tag="VFN42_00915"
                     /inference="COORDINATES: protein motif:HMM:NF021536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HET8995204.1"
                     /translation="MSTTGLEVFDRTLHLTNIWLDEVMEAVGPDRKLAWHVLCAVLRT
                     LRDRLPPELAAHLAAQLPLLVRGAYYERFQVDHRPVAIDSEAAFLDHVAAQLEGTRPI
                     NLRKATGIVLRVLQEHVAPGMTEKLKHALPHDIRALWPAEAALHP"
     gene            2580..>3627
                     /gene="groEL"
                     /locus_tag="VFN42_00920"
     CDS             2580..>3627
                     /gene="groEL"
                     /locus_tag="VFN42_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET8995205.1"
                     /translation="MPAKQVLFRSTARDKILRGTTQLADAVRVTLGPRSKAVLIQKKW
                     GAPIVCNDGVTIAKEFELKDAEENLGAQMLRQAAEKTGELVGDGTSTATVLAQAIYAD
                     GVRNVVAGASAIDLKRGLDRAARAAVDALRAMSRPVQTRKEKAQVAIISAHNDPAIGE
                     LVADAMEKVGNEGVITVEESKTTETVLEVVEGMQFDRGFLSPYFMTNTEKMQAELDDA
                     YILLCDHKIGTLKDLVPLLDEVAKSGRRLLVVAEDVEGEVLATLIVNQLRGSLRSCAV
                     KAPGFGDRRKAMLQDMAVLTGGMLIAEELGLKLESAKLHDLGQAKRVVVDKDNTTIIG
                     GAGNRADIDVRVQQI"
BASE COUNT          593 a         1279 c         1212 g          543 t
ORIGIN      
        1 cctcccaacg caagggcaac catctcgccc gaggagcagt tctggcatcg gtttcgtgtc
       61 gccgcgaccg ccgtcttcga aagccaccca ccaccggcaa ggagaacccc accggcatgc
      121 cagcccgtat cgaggattac gccctgatcg gcgattgcga aaccgccgcc ctgatcggtc
      181 gtgatggctc gatcgactgg ctctgcctgc cgcgtttcga cgccccctcc tgcttcgccg
      241 ccctgctcgg cggccccgaa cacgggcggt ggaagatcgc cccggccggc acctgccgca
      301 ccagtcgcag ctaccgccat gacacgctgg tgctggatac cgtgttcgag accgacaccg
      361 gcgcctgccg cctcaccgac ttcatgccgc tgcgcgacgg caattccgac ctggtccgca
      421 tcgtcaccgg gctgcgcggc agcgtcgatc tgcgcatgga gctgaccatc cgcttcgact
      481 acggccgctc catcccctgg gtccgtcgca tggacgacgg cagcctgcgg gcgatcgccg
      541 gcccgcatat gctggtgctc cagacccgcg tgccgctgca ggggcagggc ctgtccacca
      601 ccagcgaatt caccctggcg gcgggccaga ccgaatcctt caccctgacc tacggcccgt
      661 cccacctgcg cctgcccatg ccggccgacc cggccgaggt gctgcccgcc accgaggcct
      721 tctggcgcga ctgggcgtcc cactgcaccg tgcgcggccc gtacgcggac gtcgcgcggc
      781 gttcgctgat cacgctgaag gcgctgacct tcgatccgac cggcgggatc gtcgccgcgg
      841 ccaccacctc gctgccggaa ttcctcggcg gcgcccgcaa ctgggactac cggttctgct
      901 ggctgcgcga cgccaccttc accctgctgg ccctgatggg cgccggctac tacgaggagg
      961 cctatcgctg gcgcgactgg ctggtgcgcg ccgccgccgg cagcccgtcg cagatccaga
     1021 tcatgtatgg cctggccggc gaacgcgacc tgaccgagcg cgaactgccc tggctgcccg
     1081 gctacgccaa ctccgccccg gtgcgcatcg gcaacgccgc cgccgagcaa ctgcagctgg
     1141 acgtgttcgg cgaggtcgcc gacgcgctgc accacgcgcg caagggcgag cttggcccca
     1201 gcgaaaccgc ctggcatctg cagcagacgc tgaccgagca tctggagaag atctgggccg
     1261 ctcccgatga agggctgtgg gaagtgcgcg gcccccgcca gtgcttcacc cattccaagg
     1321 tgatggcctg ggtggcgttc gaccgtgcgg tgaaggcggt ggaggatttc ggcctggacg
     1381 gcccggtgga ccgctggcgc gcgctgcgcg accacgtgca cgaggaaatc tgccggcgcg
     1441 ccttcaatgc cgaactcggc agcttcgtgc agagctatga cagccggcag ctcgattcca
     1501 gcctgctgct gatgccgctg gtcggtttcc tgccgccgga cgacccgcgc atgatcggca
     1561 ccgtcgccgc gatcgagcgg catctggcgg tggacgggct ggtgctgcgc tacgataccg
     1621 ccgccactga tgacggcctg gcgccgggcg aaggcgcctt cctcgcctgc agcttctggc
     1681 tggcggacaa tttggtgctg cagggccgcg tgcccgaggc gcgggagcgc ttcgagcgcc
     1741 tgatcagcct ggccaacgat gtcggcctgc tcgccgagga atacgacccg cgcgccggcc
     1801 gcatgctcgg caacttcccc caggccttct cgcacgtggc gctggtcaac accgcgctga
     1861 acctgtcgcg gtgggaaggc cccgccgacc agcgcggcca ggaggagacg gcgcgggcct
     1921 gaatcccgct tcccgcgccg tatcaggcta gggatgcagc gccgcctcgg ccggccacag
     1981 cgcgcggatg tcgtgcggca gagcgtgctt cagcttctcg gtcatgccgg gcgccacatg
     2041 ctcctgcagc acgcgcagca cgatcccggt cgccttgcgc agattgatcg gacgcgtgcc
     2101 ttccagttgc gcggcgacgt ggtcgaggaa cgcggcctcg ctgtcgatcg ccaccgggcg
     2161 atggtcaacc tggaagcgtt cataatacgc gccacgcacc agcagcggca gttgtgccgc
     2221 gagatgcgcc gcaagctccg gcggcagccg gtcgcgcagc gtgcgcagca cggcgcacag
     2281 cacgtgccag gcaagcttgc ggtccggacc caccgcctcc atcacctcgt cgagccagat
     2341 attggtcagg tgcagcgtcc tgtcgaacac ctcgagaccg gtcgtgctca tcgcagccct
     2401 ccaccggatt gccgcggcag cggcggttct ggacgtcccg gcaattgacg agcgagccgc
     2461 cccgccgtgc attgatctgt atcaaatatc ccgccgaagc ggccatggat ttgacttgca
     2521 tcaatgaact tcctgccagc ccgcggctat ccgaggttcg gcttcgcagg aacccggtca
     2581 tgcccgccaa gcaagtgctg ttccgctcca ccgcgcgcga caagatcctg cgcggcacca
     2641 cgcaactggc cgacgcggtg cgcgtcaccc tggggccgcg ctcgaaagcg gtgctgatcc
     2701 agaagaaatg gggcgcgccg atcgtgtgca acgacggcgt caccatcgcc aaggaattcg
     2761 agctgaagga tgccgaggag aatctcggcg cgcagatgct gcgccaggcg gcggaaaaga
     2821 ccggcgaact ggtcggcgac ggcaccagca ccgccaccgt gctggcgcag gcgatctatg
     2881 ccgatggcgt gcgcaacgtg gtggccggtg ccagcgcgat cgacctgaag cgcggcctgg
     2941 accgcgccgc cagggccgcg gtcgacgcgc tgcgcgccat gtcgcgtccc gtgcagacgc
     3001 gcaaggagaa ggcgcaggtc gccatcatct ccgcgcacaa cgatccggcg atcggcgagc
     3061 tggtggccga cgcgatggag aaggtcggca atgagggcgt catcaccgtg gaggaatcca
     3121 agaccaccga aaccgtgctg gaagtggtcg agggcatgca gttcgaccgc ggcttcctgt
     3181 caccctattt catgaccaac accgagaaga tgcaggcgga gctggacgac gcctacatcc
     3241 tgctgtgcga ccacaagatc ggcacgctga aggatctggt gccgctgctg gacgaggtgg
     3301 cgaaatccgg ccgccgcctg ctggtggtgg ccgaggacgt cgaaggcgaa gtgctggcga
     3361 cgctgatcgt caaccagctg cgcggctcgc tacgcagctg cgcggtgaag gcgccgggct
     3421 tcggcgaccg gcgcaaggcg atgctgcagg acatggcggt gctgaccggc ggcatgctga
     3481 tcgccgagga actcgggctg aaactggaaa gcgccaagct gcacgatctg ggccaggcga
     3541 agcgcgtggt ggtggacaag gacaacacca cgatcatcgg cggcgccggc aaccgcgccg
     3601 acatcgatgt gcgcgtgcag cagatcc
//
LOCUS       DASSZH010000049         2665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1552246, whole genome shotgun sequence.
ACCESSION   DASSZH010000049 DASSZH010000000
VERSION     DASSZH010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552246"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..883)
                     /locus_tag="VFN42_00925"
     CDS             complement(137..883)
                     /locus_tag="VFN42_00925"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET8995206.1"
                     /translation="MDAQANPVTQVAAALRGMRVVDLSPLLERGIPNWPMHPPLVIDK
                     AREKERDGYYCQLLMISEHTGTHVDAPVHFHPQMENASIDCFSPDTLIAPAVVYDFAD
                     RNLKPGELITRDMVEAYERDHGVAVARGEIALINFGWMQRYWRTDSQSTWFVKNAPGI
                     AEDAVIFFKERGIRAIGCDTVACDMAVVDGQGLDTPGHITHWLPNRILIIEMLTNLDK
                     LSPRCLFVATPLKIREGSGSPIRPLAFCDN"
     gene            complement(911..2107)
                     /locus_tag="VFN42_00930"
     CDS             complement(911..2107)
                     /locus_tag="VFN42_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995207.1"
                     /translation="MNMSHTLAPPEATAPPAPRGGLRAALFAQRRLRLLPMILVLLVV
                     WAILQALTGGLFLSARNLTNLSGQVAITAMLAAGAVMVMVPGYIDLSLGATVAASAVV
                     AAFASNTFGLGFWPTVIITLGFGLLVGLWHALWVAWLKVPSFIVTLASLLAIRGISLV
                     ITGAETISPGDKMLVISDASLSTTISIVVLVAVWLAFSALQWREWRAQLAAGIRASFA
                     ANVGLPAGAFGIVALAAVVTAAGYRGIPMPVALVMAIIAFTSVVLRYTGFGRHLYAIG
                     GNRQAAQLAGIDIRAHASWVFALMGLLYGLAGLVLVARLDSAPPTAEEGLELNVIAAA
                     VIGGTSLLGGRGTVAGAALGALLMESLANGMSLMNLPSAYQSITVGLVLLLAVYVDLR
                     GRGGAD"
     gene            complement(2104..>2665)
                     /locus_tag="VFN42_00935"
     CDS             complement(2104..>2665)
                     /locus_tag="VFN42_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8995208.1"
                     /translation="GREVRLRDPADAIRHGIAYLPAERKRDGLITAHSIATNMSLVAL
                     PKLSRMGAIDRRAELQSIRRYIEDLRVKCDSIDQPIEQLSGGNQQKVVAAKWLMTEPD
                     VFVLEEPTRGVDVNARIDFYNLINDLTAAGKGVILVSTDLPEVLGMADRVLAMHEGRI
                     VREWPRGEATEELVMLHAAGEHEAAA"
BASE COUNT          479 a          898 c          888 g          400 t
ORIGIN      
        1 cgtcgccttg ccgttgcccc agccttgccc gacgcagccg gccccgaaca ccaacgccac
       61 cttgtcctgc aaccggcggc gcccggactg ccaggggaca gcggacgcat cgcctgcttg
      121 gttcgtcgac atcatctcag ttgtcgcaga acgccagggg acggatgggc gaaccggatc
      181 cctcgcggat cttcagcggc gtcgccacga acagacagcg cggcgacagc ttgtcgaggt
      241 tggtgagcat ctcgatgatc aggatccggt tcggcagcca gtgggtaatg tgacccggcg
      301 tgtcgaggcc ctggccgtcc accaccgcca tgtcgcacgc caccgtgtcg cagccgatgg
      361 cacggatgcc gcgctccttg aagaaaatga ccgcatcctc ggcaatgccg ggcgcattct
      421 tgacgaacca ggtgctctgg ctgtcggtcc gccagtagcg ctgcatccag ccgaagttga
      481 tcagcgcgat ctcgcccctg gcgaccgcga cgccgtgatc gcgctcatag gcctcgacca
      541 tgtcgcgcgt gatcagctcg ccgggcttga ggttgcggtc ggcgaaatca tagacgacgg
      601 cgggcgcgat cagcgtgtcc ggcgaaaagc agtcgatcga ggcattctcc atctgtggat
      661 ggaagtgcac cggcgcatcg acatgcgtgc cggtatgctc ggaaatcatc agcagctggc
      721 agtagtaacc gtcacgctcc ttctcgcgtg ccttgtcgat caccagcggt ggatgcatcg
      781 gccagttcgg gatgccgcgc tccagcagcg gcgacagatc gaccacgcgc atgccgcgca
      841 gcgcggcggc gacctgggtg acgggattcg cttgagcgtc catggcaatt cctccgagcg
      901 cgatgggtgg tcagtcggcg ccgccgcggc cacgcagatc gacgtagacg gcgagcagca
      961 gcaccagccc gacggtgatc gactgataag cggacggcag gttcatcagg ctcatgccgt
     1021 tcgcgagact ctccatcaac agcgccccga gtgcggcgcc agcgacggtg ccgcgtccgc
     1081 cgagcaggct ggtgcccccg atcacggcgg cggcgatgac attgagctcc agcccttcct
     1141 ccgccgtggg cggcgcgctg tccagccgcg ccaccagcac gaggccggca agcccataga
     1201 gcagccccat caaggcgaac acccacgagg catgcgcgcg gatgtcgatg ccggcgagct
     1261 gcgcggcctg ccggttgccg ccgatggcgt agagatgccg cccaaagccg gtgtagcgca
     1321 gcacgacact ggtgaaggcg atgatcgcca tgacgagtgc caccggcatc ggaatgccgc
     1381 gatagccggc cgctgtcacc acggcggcga gcgcgacgat gccgaacgcg ccggccggca
     1441 ggccgacatt ggcggcgaag gaggcgcgga tgccagccgc aagctgcgcg cgccattcac
     1501 gccactgcag ggcagagaat gccagccaca ccgcgacaag cacgacgatg ctgatcgtcg
     1561 tggacagcga ggcatccgaa atcaccagca ttttgtcgcc cggcgagatc gtctccgctc
     1621 cggtgatcac cagtgagatg ccacggatgg caagcaggct ggccagcgtg acgatgaaag
     1681 agggtacctt cagccatgcc acccacagag cgtgccacag cccgaccagc aggccgaagc
     1741 cgagcgtgat gatcacggtc ggccagaagc caaggccgaa tgtgttgctg gcgaaggcgg
     1801 ccaccaccgc cgaggcggcg acggtggcgc ccagcgagag gtcgatataa cccggcacca
     1861 tgaccatcac cgcgcccgcg gcaagcatcg cggtgatcgc gacctggccg ctcaggttgg
     1921 tcaggttgcg ggcgctgagg aacaggccgc cggtcagggc ttgcaggatg gcccagacca
     1981 ccagcaacac caggatcatc ggcagcagcc gcaaacgccg ctgcgcgaag agggcggcac
     2041 gcagtccgcc acggggtgcg ggaggggcag tggcctcggg cggggcaagc gtgtgcgaca
     2101 tgttcatgcg gcagcctcat gttcgccggc agcgtgcaac atcaccagtt cctccgtcgc
     2161 ctcaccgcgc ggccactccc gtacgatccg cccctcgtgc atggccagca cgcggtcagc
     2221 catgccgagc acctcgggaa ggtcggtgga gaccaggatg acacccttgc cggccgcggt
     2281 caggtcgttg atcaggttat agaaatcgat gcgtgcgttg acatcgacgc cccgcgtcgg
     2341 ttcctccagc acgaacacgt ccggttcggt catcagccac ttcgcggcga cgactttctg
     2401 ctggttgccg ccgctcagct gctcgatcgg ctggtcgatg gagtcgcact tgacccgcag
     2461 atcctcgatg tagcggcgga tgctctgcaa ctcggcgcgg cggtcgatcg cgcccatgcg
     2521 gctcaacttc ggcaacgcca ccagcgacat gttggtggcg atgctgtgcg ccgtgatcag
     2581 cccgtcgcgc ttgcgctccg caggcaggta ggcgatgccg tggcggatcg catccgcggg
     2641 gtcgcgcagg cggacctccc gcccg
//
LOCUS       DASSZH010000050         2079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1637425, whole genome shotgun sequence.
ACCESSION   DASSZH010000050 DASSZH010000000
VERSION     DASSZH010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1637425"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VFN42_00940"
     CDS             complement(<1..575)
                     /locus_tag="VFN42_00940"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET8995209.1"
                     /translation="MSYRQPHLLAIEGLEPPEIGHLLDQAESYALLNRSGKTQRDLLR
                     GRTLINLFFEDSTRTRTSFELAGKRLGADVINMSVSTSSVNKGETLLDTAATLNAMHC
                     DLLVVRHAQSGAPNLLAQKVDAAVINAGDGTHEHPTQALLDALTIRRRKGRLEGLTVA
                     ICGDVLHSRVARSNIHLLTTMGSAVRVVGPPT"
     gene            complement(708..1346)
                     /locus_tag="VFN42_00945"
     CDS             complement(708..1346)
                     /locus_tag="VFN42_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995210.1"
                     /translation="MPERIGRLLILVQLLIGYGKTFAGILRQQVVTPWNGLASQARFG
                     TSGTARLLLRIGRALKLAAMPGSRLRRRTAAGPDVRTPLEVAPVQPRCFAHRSTTNSA
                     RRAAVRGAVLDNLSDVGLPSEGQIAAMIRRGRAGAIVAALCRDLGLVPAVVREQQWKD
                     LCGAAVRFGDNFDRRIRGAMWRIGVSMTAAPAAREPRSGCALECAGEWPAPV"
     gene            1690..1821
                     /locus_tag="VFN42_00950"
     CDS             1690..1821
                     /locus_tag="VFN42_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995211.1"
                     /translation="MRQAVAGESRQPGGILAALRCSPLVGADFDLARPREAGRTLDL"
     gene            1818..>2079
                     /locus_tag="VFN42_00955"
     CDS             1818..>2079
                     /locus_tag="VFN42_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009573743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HET8995212.1"
                     /translation="MTRYLLDTSIISKAVKPQPSEPLLAWMAAQRDDTLFIASLTLAE
                     IRRGILDLPAGRRRTALESSSRKPFRDLPPMIRTTDKYGKHSD"
BASE COUNT          331 a          661 c          767 g          320 t
ORIGIN      
        1 gtcggcggcc cgacgacgcg caccgcgctg cccatcgtgg tgagcaggtg gatgttggag
       61 cgggcgaccc ggctgtgcag cacgtcgccg cagatcgcca cggtgagacc ctccagcctg
      121 cccttgcggc ggcggatggt cagcgcatcc agcagcgcct gggtggggtg ttcgtgcgtg
      181 ccgtcgccgg cgttgatcac cgcggcatcg actttctgcg ccagcaaatt gggcgcgccg
      241 gactgggcgt ggcgcaccac cagcaggtcg caatgcatgg cgttgagcgt ggcggcggtg
      301 tccagcagcg tctcgccctt gttcacgctg gaggtggaga ccgacatgtt gatgacgtcg
      361 gcgccgagcc gcttgccggc cagctcaaag ctggtgcggg tgcgggtgga gtcctcgaag
      421 aacaggttga tgagggtgcg gccgcgcagc aggtcacgct gggttttgcc cgagcggttg
      481 agcagcgcgt agctctccgc ctggtcgagc aggtggccga tttcgggggg ttcgagtccc
      541 tcgatcgcca ggaggtgtgg ttgccggtag gacacggcgt ctggtgacag gttttgcggg
      601 gcggcgtcaa tccggggcgc gcgggcgcgc gcggcgaaac aggcagcgcc gaacgcgggg
      661 ccaccaggtg atggtcccgt gcgcaaccgc gtttcaccct gccggtgtca gaccggagcg
      721 ggccactccc cggcgcattc cagcgcgcaa ccgctccgcg gttcgcgcgc ggccggcgcc
      781 gcggtcatac tcacgccgat ccgccacatc gcgccgcgga tccgtcgatc gaaattatcg
      841 ccaaacctga cggcggcgcc gcataggtcc ttccattgct gctcgcggac gacggcgggc
      901 accaggccga ggtctcggca gagtgccgcg acgatcgcgc cggctctgcc acggcggatc
      961 atggcggcga tctgtccctc gctgggcagg ccgacatccg aaaggttgtc gagaacggcc
     1021 ccacgcactg ccgcacgccg agcagagttg gtggtggacc ggtgggcgaa gcagcgcggc
     1081 tgcaccggcg caacttccag gggagtccgc acgtccgggc cggcggcagt ccgccgccgc
     1141 agccgggaac ccggcatggc ggccagcttc agcgctcggc cgatacgaag gaggaggcga
     1201 gccgtaccgg aggtgccgaa gcgggcctgc gaggccaagc cgttccacgg cgtcaccacc
     1261 tgctggcgca ggatgccggc aaaggtcttg ccgtagccga tgagaagctg gaccaggatg
     1321 agcaggcgac cgatgcgttc gggcacggtg gatggggctc cggtttgggg tgggtgccgc
     1381 ggtgggctgc ggcgggtcgg gaacatgtat agaacacaac cccagattgt gtcaagggaa
     1441 ttttttctac cgcgaccggg aaggtggcga tccgtccggt ggagatgacg aagcaggtcg
     1501 ctgccgggcg ggaccgcccg ataagcttta acaggagcgg cagacgtgaa ttctggggtc
     1561 atgggcggcc cttgagccgg caagatccgg aatcgcatgg aggcagcgaa gtggggagtc
     1621 gcgcgcagaa acgcgccatt tagcagtatc ggtccaggca gacggaccgc ggcatcgtcc
     1681 ggttcgcagg tgcggcaggc ggtcgccggc gagtcgcggc aacccggcgg catcctggcg
     1741 gctttgcgct gctcgccgct ggtgggcgcc gattttgacc tggcgcgccc gcgcgaggcg
     1801 gggcgaacgc tcgacctgtg acgcgctatc tgctcgacac cagcatcatc agcaaagcgg
     1861 tgaagccgca gccgtcggag ccgttgctcg cctggatggc ggcccagcgt gacgacacgc
     1921 tgttcatcgc ctccctgaca ctggcggaga tacgtcgcgg cattctggac ctgccggcag
     1981 gccggcgacg caccgcgctg gagtcctcat cgcgcaagcc tttccgcgat ctgccgccta
     2041 tgatccgcac caccgacaaa tacggcaagc acagtgacc
//
LOCUS       DASSZH010000051         3919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_785333, whole genome shotgun sequence.
ACCESSION   DASSZH010000051 DASSZH010000000
VERSION     DASSZH010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785333"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1320
                     /locus_tag="VFN42_00960"
     CDS             <1..1320
                     /locus_tag="VFN42_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995213.1"
                     /translation="GSALATWEQEVAAGPCGFSIDSRGVRERFNLPQFAGQLFVHAIG
                     AAGHDVVKYALDTYASAGGASLSCTHDANAWPSDRYAGLPAPRADEQVVLWLQNSHAV
                     PIPAGTIALDRMGAERPVTLNREIGPFATYALDVAALLPDVRWPAQIELRAGRHMVRP
                     RYEVIRGGRTRIAHVNVERADLRPDPGIPALPPQLGRGYLLPFPVLPRARFRSIVQPT
                     PMAETQTFLPVRLDVFDTEGRCVTQRFLGCLPRDHAVAVDFDDIDVASGHAELVYDFR
                     DGGSADGWLHALFRYEDRGSGHAAESSFGGHIFNTLMTYKDEPQSYSGPPPGLSTRLF
                     LKLGDARRHSFAVLIYPASAAWHPRSSTTLILHDGAGQVLAEKPLAIACSGSAMVWPH
                     QVFDAGLLERAGANGYVLVRDTTCRLFGYHGLMDDAGGFSLDHMFGF"
     gene            complement(1453..2886)
                     /locus_tag="VFN42_00965"
     CDS             complement(1453..2886)
                     /locus_tag="VFN42_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HET8995214.1"
                     /translation="MTAALARELGAALAGTGAVSDGPGSGMRFAELRAAAEAIAGTLA
                     RHGLAPAEPVHLLIGNRAADLAGMLGIWQAGGVVVPVHRSALAATTARIAALSAARFA
                     VDGSAVTAIASEPPTRRPLLHDAALVIFTSGSTGLPKGVVIGHERMAGKIAVLDRLLQ
                     LRPTDVVSVPLQLTFIFGIWVSFVTLRAGACLALVPRFSAAAQQAALQAGATVMAAVP
                     SMLRTMAAGDALHGNGPRLVLAGGEPLGPEVRRAVAQAWPGTEIRDLYGLTETGSSDF
                     CMMRAETATHGNPIGTPTSEVAFRIADPAGAAVPEGDAGELQIRSPFGMLGYLDAPEA
                     TEASFQSGYFRTGDLARVEPDGSVSLVGRLKEIVSRGGNKVAPLEIDNLLGTHPDIAA
                     ALTAGVPDPRLGEALCTVVVPRPGATIDDPALRRWLGERLERFKLPDTLSVEDTLPVG
                     PTGKASRAALRARFAEREPAGTRTSPR"
     gene            complement(2883..3704)
                     /locus_tag="VFN42_00970"
     CDS             complement(2883..3704)
                     /locus_tag="VFN42_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET8995215.1"
                     /translation="MDFATIAYETLGPVARITLSRPERANAMNAAMLDEISAALDLAE
                     QDDAVRAVIVRGAGAAFSSGFDLKEQMERQPKGVGPWRAILRKDFDVPMRFWHLSKPT
                     IAAVRGPCLAGACELMLACDVTIAAEDAFFGEPELKFGAGIVVMILPWIVGPKLAKEI
                     ILLGEDRISAVRAREIGMINRVVPAAELDAAALAMARNMAAIDPALVRETKRSINRAI
                     EVRGMLDALEAALASDMLIEGEGSPDKARFMDIARQQGLRAALAWRDARFTPETP"
     gene            complement(3738..>3919)
                     /locus_tag="VFN42_00975"
     CDS             complement(3738..>3919)
                     /locus_tag="VFN42_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995216.1"
                     /translation="TQRIKPGKASLIGGIAVAFAALVLWTAIAHDLAADSVPVLLLGL
                     LVAVGIGTWIRAADL"
BASE COUNT          583 a         1349 c         1375 g          612 t
ORIGIN      
        1 ggaagcgcgc tggccacctg ggagcaggag gttgccgcag gtccatgcgg gttctcgatt
       61 gacagccgcg gcgtgcgcga gcgcttcaac ctgccacagt tcgccggaca gttgtttgtg
      121 catgcgatcg gtgccgccgg gcatgacgtg gtgaagtatg cgttggacac ttacgcctcg
      181 gcgggtggcg ccagcctgtc ctgcacgcat gacgccaatg cctggccatc cgaccgctat
      241 gccggcctgc cggcgccccg ggcagacgag caggtggtgc tgtggctgca gaacagccac
      301 gccgtgccga ttccggccgg caccatcgcg ctggaccgca tgggtgcgga acgcccggtg
      361 acgctgaacc gggagatcgg tcccttcgcc acctacgcgc tggatgtggc ggcgctgctg
      421 ccggatgtgc gctggccggc gcagatcgag ttgcgtgccg gccggcacat ggtgcgtccg
      481 cgctacgagg tgatccgcgg cggccgcacc cgcatcgccc atgtgaacgt ggagcgcgcg
      541 gatttgcggc ccgacccggg cattccggcg ctgccaccac agctcggccg tggctacctg
      601 ctgcctttcc cggtgctgcc gcgcgcgcgc ttccgcagca tcgtgcagcc gacgccgatg
      661 gcggagacgc agacgttttt gccggtgcgg ctggatgtgt tcgacaccga aggacgctgc
      721 gtgacgcagc gcttcctcgg ctgcctgccg cgcgaccacg cggtggcggt ggatttcgac
      781 gatatcgacg tggcgagcgg ccacgcggaa ctggtgtatg atttccgcga cggcggcagc
      841 gcggacgggt ggttgcatgc gctgttccgc tatgaggacc ggggttccgg ccacgccgcg
      901 gaatccagtt tcggcgggca catcttcaac acgctgatga cctacaagga cgagccgcaa
      961 tcctattccg gcccgccgcc ggggctatcg acgcggctgt tcctgaagct gggcgatgcg
     1021 cgccggcaca gtttcgcggt gctgatctat ccggcgtcgg cggcatggca tccgcgctcc
     1081 agcaccacgc tgatcctgca tgacggtgcc ggacaggtgc tggcggagaa gccgctggcg
     1141 attgcctgct cgggttcggc gatggtctgg ccgcaccagg tcttcgacgc cggactgctg
     1201 gagcgcgccg gtgccaacgg ttacgtgctg gtgcgcgata ccacctgccg gctgttcggc
     1261 tatcacgggc tgatggacga tgccggcggg ttctcgctgg accacatgtt cgggttctga
     1321 cgccaatccg cgtaaccctc gacccatgtg tcatgcccgc gcttgacgcg ggcacccacg
     1381 tcttttctgt gtgcgcccga gagaagacat gggtagccgg cctgcgccgg ccatgacacc
     1441 ggaaagtcgg cgtcagcgcg ggctggtacg agtcccggcc ggttcgcgtt cggcgaagcg
     1501 cgcgcgcagc gccgctctgc ttgccttgcc ggtggggccg accggaaggg tgtcttccac
     1561 cgacagcgta tcgggaagtt tgaaccgctc caggcgctcg ccgagccagc gccgcagggc
     1621 agggtcgtcg atcgtggcgc cgggccgggg gaccacgacg gtgcacagcg cctcacccaa
     1681 gcgcgggtcc ggcacgccgg cggtcagcgc ggcggcgata tccgggtggg tgcccagcag
     1741 gttgtcgatc tccagcggcg caaccttgtt gccgccgcgc gacacgatct ccttcagccg
     1801 gccgaccagc gacacgcttc catccggctc cacgcgggcg agatcgccag tgcggaaata
     1861 gccggactgg aaggaggctt ccgtcgcttc cggtgcgtcg aggtagccca gcatgccgaa
     1921 gggcgagcgg atctgcagct ccccggcatc accctcggga accgccgcac ctgccggatc
     1981 ggcgatccgg aaggcgactt cggaagtcgg cgtgccgatc gggttgccgt gcgtggcggt
     2041 ctcggcccgc atcatgcaga aatcgctcga gccggtctcg gtgagtccgt acaggtcgcg
     2101 gatctcggtg cccggccagg cctgggcgac ggcgcggcgc acctccgggc ccagcggctc
     2161 gccgcccgcc agcaccagcc gcggcccatt tccgtgcagc gcatcgccgg ccgccatggt
     2221 gcgcagcatg gagggaaccg cggccatcac cgttgccccc gcctgcaagg cggcctgctg
     2281 tgccgcggcg gagaagcgcg ggacaagggc gagacatgcg ccggcccgca gcgtgacgaa
     2341 ggagacccag atgccgaaga tgaaggtgag ctgcagcggc accgagacga catcagttgg
     2401 ccgcaattgc agcagccggt cgagcacggc gatcttgcca gccatccgct cgtgcccgat
     2461 caccacgcct ttcggcaggc cggtgctgcc ggaagtgaag atcaccaagg cggcgtcgtg
     2521 cagcagcggg cgccgggtcg gaggctctga ggcgatggcg gtgaccgcgc tgccatccac
     2581 ggcaaagcgt gcggcggaca gggcggcgat acgggctgtg gtggcggcca gggcactgcg
     2641 atgcaccggc acgaccacgc cgccggcctg ccagatgccc agcatgccgg caagatccgc
     2701 ggcgcggttg ccgatcagca gatgcacggg ttctgccggc gcaagcccgt gccgggccag
     2761 cgtgccggcg atcgcctccg ccgccgcgcg cagctcggcg aagcgcatgc ccgagccggg
     2821 tccgtcgctg accgcgccgg tgccggccag tgccgcaccg agttcgcgag ccagcgctgc
     2881 ggtcatggcg tttccggggt gaagcgcgca tcgcgccagg cgagcgccgc gcgcaatccc
     2941 tgctggcggg cgatgtccat gaagcgcgcc ttgtcgggtg aaccctcgcc ttcgatcagc
     3001 atgtcggagg ccagcgccgc ttcgagcgcg tccagcatcc cgcgcacctc gatcgcccgg
     3061 ttgatgctgc gcttcgtctc acgcaccagc gccggatcga tcgcggccat gttgcgcgcc
     3121 atcgccaagg ccgccgcatc cagctctgcc gcgggaacca cgcgattgat catgccgatc
     3181 tcgcgcgcgc gcaccgcgct gatgcggtct tcgccgagca ggatgatctc cttggcgagt
     3241 ttcggtccga cgatccaggg caggatcatc acgacaatgc cggcgccgaa cttgagttcc
     3301 ggctcgccga agaaggcgtc ttcggcggcg atcgtcacat cgcaggccag catcaactcg
     3361 caggctccgg caaggcaggg tccgcgcacc gctgcgatgg tcggcttgga cagatgccag
     3421 aagcgcatcg gcacatcgaa atccttgcgc aggatcgcgc gccatggccc gacgcctttc
     3481 ggctggcgtt ccatctgctc cttgaggtcg aagccggagg agaaggcagc gcctgcgccg
     3541 cgcacgatga cggcccgcac cgcgtcgtcc tgctccgcga gatcgagcgc ggcggaaatc
     3601 tcgtcgagca tcgcggcgtt catcgcgttc gcccgttccg ggcgggacaa ggtgatgcgt
     3661 gcgaccgggc ccagcgtttc ataggcgatc gtggcgaaat ccatccggct tcccccgccg
     3721 cgtcggcgtt tggcctgtta cagatccgcg gcgcggatcc aggtgccgat accgaccgcc
     3781 accagcaggc ccagcagcag caccggcacg ctgtccgccg ccaggtcgtg cgcgatcgcc
     3841 gtccacagca ccaatgcggc gaatgcgacc gcgatgccgc cgatcaggct cgccttgccg
     3901 ggcttgatcc gctgcgtgc
//
LOCUS       DASSZH010000052         2079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1262596, whole genome shotgun sequence.
ACCESSION   DASSZH010000052 DASSZH010000000
VERSION     DASSZH010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262596"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1058)
                     /gene="nrfD"
                     /locus_tag="VFN42_00980"
     CDS             complement(78..1058)
                     /gene="nrfD"
                     /locus_tag="VFN42_00980"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET8995217.1"
                     /translation="MPEGIDLGGERGRWAGRTYYGRPQVKPAPFENAVVGTYVFLAGL
                     SGATQLLSSLLDLTRGPAAGRAVRRGRLLSMLAPTIGAACLIFDLHTPKRFYNMLRVA
                     KGTSPMSIGTWILMAFSSSSALIAAAHLASDYVPGFRWLRSVARAAQVPAAAAGAGLS
                     TYTAALLSATSTPLWAAAPKGLAVRFGASAIASGAAALGLGERRSRIGRDLDSIAIAA
                     LATELAATLASEERYRRTGVDRALHSTPGVLDQLGGTGIGVLLPLGLYAASLLLTRRR
                     SRTLTTAASLAVLGGSLAMRIGVMAAGDESARRPEISMGFAQPENLPKVS"
     gene            complement(1072..1959)
                     /locus_tag="VFN42_00985"
     CDS             complement(1072..1959)
                     /locus_tag="VFN42_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET8995218.1"
                     /translation="MEMHPRVLNYTQSDLDGVIPDEAELTANVRIVPGNSYGFFTDTT
                     VCIGCKACEVACKEWNNLPADHLGLTGDSYDNTGALSANTWRHVNFIETDAVRTDLEQ
                     PFQSGWLMMSDVCKHCHNAPCLEACPTGALFRTEFDTVVVQQDICNGCGYCVPACPFG
                     VVDVSTLDGKAHKCTLCYDRLKGGLEPACAKACPTDSIQFGEISELHLRARQRVERLH
                     EVGVESAYLYGVPDGPGATGGLDHLNAFFLLTARPETYNLPSAPTRPANRVVPSLTAG
                     LTAVAGLALAALAIFTRPH"
BASE COUNT          333 a          717 c          684 g          345 t
ORIGIN      
        1 gcaacccgtc gtggcgggcg caggcgctcc acccacgact tgctgactgg ctccaacaag
       61 gccctcccca caacgttcta cgataccttc ggcaagttct ctggctgcgc gaagcccatg
      121 ctgatctccg gccgccgcgc cgattcgtct ccggccgcca tcacgccgat ccgcatcgcc
      181 aggctgccgc ccagcaccgc caacgacgcc gctgtggtca gcgtccgcga gcgccggcgt
      241 gtcagcagca atgacgccgc ataaagcccc agcggcagca gcacgccgat accggtgcca
      301 ccaagctggt ccagcacccc gggcgtgctg tgcagcgcgc gatccacgcc cgtccgccgg
      361 tagcgttcct cggatgccag cgtcgccgcc aattcagttg ccagtgcggc aatggcgatg
      421 ctgtccaggt cacgtccaat gcggctgcgc cgttcgccca atcccagcgc cgcagcgccg
      481 gaagcgatcg ccgatgcgcc aaaccgtacc gccagtccct tgggtgcagc ggcccaaagc
      541 ggtgtgctcg tcgccgagag cagcgccgcg gtgtaggtgc tcagccccgc acccgccgcc
      601 gcggccggca cttgcgcggc ccgcgccacg ctgcgcagcc agcggaagcc cggcacgtaa
      661 tcgctggcga gatgggccgc cgcgatcagg gcgctgctgc tgctgaaggc catcaggatc
      721 caggtgccga tcgacatcgg cgatgtgccc ttggcaacgc gcagcatgtt gtagaagcgc
      781 ttcggcgtgt gcaggtcgaa gatcaggcag gccgcaccga tcgtgggcgc gagcatcgac
      841 agcagccgtc cacgccgcac cgcgcggccc gccgcggggc cgcgcgtcag gtccagcagg
      901 ctggacagca attgtgtcgc gccggacagg ccggccagga aaacgtaggt gccaacgacg
      961 gcgttctcga acggcgccgg cttcacctgc ggccggccgt aataagtgcg gcccgcccag
     1021 cgcccgcgct cgccgcccag atcgatgcct tcgggcatgc tgtcctcctt ctcagtgcgg
     1081 gcgggtgaag atggcgagcg ccgccaatgc caacccggca accgcggtca gtcccgccgt
     1141 tagactcggc accacccggt tggccggacg cgtcggtgcg ctgggcaggt tgtaggtttc
     1201 cggccgagca gtgaggagga agaaggcgtt gaggtggtcc aggccaccgg tagcgcccgg
     1261 gccgtccggc acgccgtaca ggtacgccga ctccacgccg acctcatgca ggcgttcgac
     1321 gcgctgtcga gcacgcaggt gcagctcgga gatttcgccg aactggatgg agtcggtggg
     1381 gcacgctttg gcgcaggctg gttccagccc gcccttcagt cggtcgtagc acagcgtgca
     1441 tttatgcgcc ttgccatcca gcgtgctgac atccaccacg ccgaacggac aggccggcac
     1501 gcaatagccg cagccgttgc agatgtcctg ctgcaccacc acggtgtcga attcggtacg
     1561 gaacagcgcg ccggtcggac aggcttccag gcacggtgcg ttatggcagt gcttgcagac
     1621 gtcgctcatc atcagccagc cgctctggaa gggctgttcc aggtcggtgc ggaccgcatc
     1681 ggtctcgatg aaattgacat gccgccaagt gttggcggac agcgcgccgg tgttgtcgta
     1741 gctgtcgccg gtcaggccga ggtgatccgc cggcaggttg ttccattcct tgcacgccac
     1801 ctcacaggcc ttgcagccga tgcacacggt ggtgtcggtg aagaagccgt aggaattgcc
     1861 gggaacgatc cgcacattgg cggtcagctc cgcttcatcc gggataacgc cgtccaggtc
     1921 ggactgcgtg tagttcagca cgcggggatg catttccatt gccggcgttt acctcgctgc
     1981 tttcaggcgt gttcgtgtac gcgggccctg gctgggccgg cggtcggtgt ctgtgattgc
     2041 tgcaggatac gctcttcgtc ttccagcacg tcgatcctc
//
LOCUS       DASSZH010000053         4834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1007016, whole genome shotgun sequence.
ACCESSION   DASSZH010000053 DASSZH010000000
VERSION     DASSZH010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007016"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VFN42_00990"
     CDS             complement(<1..364)
                     /locus_tag="VFN42_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995219.1"
                     /translation="MFKGFILGIVITILAGAGVGYALIRTGSVPAAARDAHPVFLEHW
                     AARTSLRATLANEAPKGPNPVPLNDANLIAGIGLFRDHCAICHGTAQGEKSPSSIAQG
                     EYPPPPQMATDGVEDDPEG"
     gene            836..1981
                     /locus_tag="VFN42_00995"
     CDS             836..1981
                     /locus_tag="VFN42_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HET8995220.1"
                     /translation="MQGDPKGYYARLEVEPWAPREAITAAYRRKARVLHPDIPVTGNA
                     AAFVAMKEAYEVLTDPLRRAAYDRAAKAPPVSPPPPPQPPPSYEYDEAEELYPEPPPP
                     MRTPKVRQPRLSDMPTWLWIGLLGVCIVGVGEAALQLNAGPAPVPAPVITPNAPFVPP
                     AAPPVPIQPVQLAGSPNAYVLPSGGDAVVWRHDPATDGFVPLGHLPPFTAVQVVRTLA
                     QHGMVEVLLSRQQAGFIDASRLTPGNSAAAHRAFCAYNAGPEPANAEVLARSGSGSLS
                     ITLVNRSGEPAVVKLRTTDGVTAASVYLSPMGSAQVSGLPAAAYQPEYAIGELWSRTC
                     NSFAAGMQAERFSGAVDLSRLSSLVIPPDLLPGVHPVDIPNKTFQNP"
     gene            1994..2905
                     /locus_tag="VFN42_01000"
     CDS             1994..2905
                     /locus_tag="VFN42_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8995221.1"
                     /translation="MAITLQESLRGVFYAPFYVALAQDAYAREGVEVRFASASHPGDA
                     AEAVLRGDVDVCWGGPMRVMQAYAQHADCDLVCFAEVVTRDPFLLVGREPRPDFRLED
                     LPGLRVATVSEVPTPWMCLQDDLRRAGIAPARLARKADGSMAENVAGLLAGSLDVIQV
                     FEPFAAELEAEGAGHVWYAAADRGPTSYTTFYARRGLLAARREELLRLVRAIARTQRW
                     LGGASADAIAAAIQGYFPELPLPRLAAACARYQRLGIWGADPVLPPAGYDRLRASLES
                     GGFVAAGTPFAVAVDNSLAQAAVATLA"
     gene            2967..3494
                     /gene="ppa"
                     /locus_tag="VFN42_01005"
     CDS             2967..3494
                     /gene="ppa"
                     /locus_tag="VFN42_01005"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HET8995222.1"
                     /translation="MFLDAIPIGNNPPEDVNVIIEVAVGGEPVKYEMNKAAGTLFVDR
                     FLYTPMRYPGNYGFVPHTLSDDGDPIDVLVANTRPLVPGAVINVRPVGVLKMEDDGGG
                     DEKILAVPVPKLTRRYEHVHNYTDLPRITLEQIQHFFEHYKDLEPGKWVRVIGWGDSA
                     EAQNLIRDAIARAKA"
     gene            complement(3507..4100)
                     /locus_tag="VFN42_01010"
     CDS             complement(3507..4100)
                     /locus_tag="VFN42_01010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8995223.1"
                     /translation="MPFPALAQPDSAPAAPVSALYAGLEAVMREGASKPFTARFAQLA
                     PVITQAFDLTAILMHSVGLHWASLADAARARLFKAFSDFTVASYVANFDEYSGQKFEI
                     LPEQRSVGADRVVASRIVRASGNTVRIDYLVHQDGPRWRITDVLLDGTISRVAVQRSD
                     FRSLLDRGGAEALIASLQSKTADLSGGARLSVVVPPR"
     gene            complement(4230..>4834)
                     /locus_tag="VFN42_01015"
     CDS             complement(4230..>4834)
                     /locus_tag="VFN42_01015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009008 - DNA-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET8995224.1"
                     /translation="AMKALNDDIQMRSDWFIPLCTGPERLRNAHGLKLHPTQKPEALL
                     HRVLLASTSPGDIVLDPFLGTGTTGVVAKRLHRHFIGLERHPAYAEAAIGRIRHTAPA
                     PHEGLAVAASKRDAPRIPFGSLVESGQVPPGTKLYDRQRRVTAVVAADGTLASGAFRG
                     SIHKVGAAVQNAPSCNGWTFWHFEREGVLQPLDVLREAAR"
BASE COUNT          750 a         1684 c         1631 g          769 t
ORIGIN      
        1 agccttccgg atcgtcttcc accccgtcgg tcgccatctg tggcggcggc ggatattcgc
       61 cttgcgcgat cgacgaggga gacttctcgc cctgcgccgt gccgtgacag atcgcacaat
      121 ggtcccggaa caggccgatg ccggcgatca ggttggcgtc gttcagcggc accgggttgg
      181 gtcccttggg tgcctcattg gcgagtgtgg cacgcaagga tgttcgggcg gcccagtgct
      241 ccaggaacac cgggtgggcg tcgcgcgccg cggccggcac cgagccggtt cggatcaggg
      301 cgtaacccac ccctgcccct gccagaatcg tgatcacgat tcccaggatg aagcctttga
      361 acattcgtgc tcctcttggt taccaggccg ttgctggccg ggatgcgttg ttgccatttc
      421 ccgtggccgg tgccttggcg aaatggcgag cggtaaacca aaggtgacgg attttgcccg
      481 ccgccaccgc ccgatcggct ggcgcagcgg attcaaacgg ggcgctgccg atggcgccaa
      541 tgccacaccc aaggatgaac gttgctcgat tgggaacagc aggcaatgcc ggacccatac
      601 tcccggcgag agctgtagtg cacgctgtat acttgtgcat tttttaatct ttcctgctgc
      661 aagattagca tcctccttaa acaacagcaa cggtttgccg ccgcgctgca accggcaccg
      721 ggcgcccgaa cccggtttcc cgccgccggc cgatcccaaa tgcgcaatct gaccattcgc
      781 ggcttgcatt tgccgcaagg caactgcaat cgggcgcact aggatgcgga cggcgatgca
      841 aggcgacccg aaaggctact acgcacggct ggaagtcgag ccctgggcac ccagggaagc
      901 gatcactgcc gcttatcgcc gcaaggcgcg ggtgctgcac ccggatatcc cggtcaccgg
      961 caacgccgcg gccttcgtcg cgatgaagga ggcgtacgag gtcctgaccg atccgctgcg
     1021 ccgggcggcg tatgaccgcg cggccaaggc gccgcctgtg agcccaccgc cgccaccgca
     1081 accgccaccg tcatacgagt acgatgaggc ggaagagctg tatcccgagc caccgccgcc
     1141 gatgcgcacg ccgaaggttc ggcaaccgcg gctgtcggat atgcccacct ggctgtggat
     1201 cggcctgctc ggcgtctgca tcgtcggggt gggggaagca gcgctgcaac tcaacgccgg
     1261 tccggcgccg gtacccgccc ccgtcatcac ccccaacgcg cctttcgtgc cgccggccgc
     1321 cccgccagtg ccgatccagc cggtgcagct ggccggttcg ccgaatgcct atgtgctgcc
     1381 ctccggcggc gatgccgttg tctggcggca tgaccccgcc accgacggct ttgtcccgct
     1441 cggccatctg ccgccgttta ccgcggtgca ggtggtccgc accctggcgc agcacggcat
     1501 ggtggaagtg ctgctcagcc ggcagcaagc cggttttatc gacgcgtccc ggctgacgcc
     1561 gggcaattcc gcggcggcgc atcgggcctt ctgcgcctac aatgccgggc ccgagccggc
     1621 caacgcggaa gtgctggccc gcagcggcag cggctcgctg tccatcacgc tggtgaaccg
     1681 cagcggcgag ccggcggtgg tgaagctgcg caccacggac ggggtgaccg ccgcttcggt
     1741 gtacctgtcc cccatgggct cggcgcaggt ctccggcctg ccggccgccg cgtaccagcc
     1801 cgaatacgcc ataggcgagc tatggagccg cacctgcaac agcttcgccg ccggcatgca
     1861 agccgagcgg ttttccggtg cggtggatct gtccaggctg tcgtcgctgg tcatcccgcc
     1921 cgatcttctc ccgggcgtcc atccggtgga tattcccaac aagacattcc agaacccctg
     1981 accggagtcc gccatggcga tcacgctgca ggagtcgctt cgcggggtgt tctacgcgcc
     2041 cttctacgtc gcgctggcgc aggacgccta tgcgcgggag ggggtggagg tccgcttcgc
     2101 cagcgcctcg cacccgggcg acgccgccga ggcagtgctg cgcggcgatg tggacgtgtg
     2161 ctggggcggg ccaatgcggg tgatgcaggc ctatgcccag cacgcggatt gcgacctggt
     2221 ctgcttcgcc gaagtggtca cgcgcgaccc gttcctgctg gtcggccggg aaccgcgccc
     2281 ggatttccgg ctggaggatc tgccggggct gcgcgtggcg actgtcagcg aggtgcccac
     2341 cccgtggatg tgcctgcagg acgacctgcg ccgcgccggc atcgcccccg cccggctggc
     2401 ccgcaaggcg gacggctcca tggcggagaa cgtggcgggc ttgctggccg ggtcgctgga
     2461 cgtgatccag gtgttcgaac ccttcgccgc ggagctggag gccgaaggtg ccgggcatgt
     2521 ctggtatgcc gcagccgacc gggggcccac cagctacacc accttctacg cccggcgcgg
     2581 cctgctggcg gcgcggcgag aggagctgct gcgcttggtg cgggccatcg cgcggaccca
     2641 gcgctggctg ggcggcgctt ccgccgacgc tatcgccgcg gccatccagg gctatttccc
     2701 ggaactcccg ctccccaggc tggccgccgc ctgcgcacgt taccagcggc tgggcatctg
     2761 gggcgccgac ccggtgctgc cgcctgctgg ctacgaccgg ctgcgcgcga gcctggaatc
     2821 cggcggcttc gtcgccgcgg gcacgccctt cgccgtcgca gtggataaca gcctcgcgca
     2881 ggcggccgtg gcgacgcttg cctagacgcc ctgctgccgc gacatacgcc aagcctttat
     2941 ccacccggct tttttggaga cccagtatgt ttctcgacgc catcccgatc ggcaataacc
     3001 cgccggagga cgtcaacgtc atcatcgaag tcgcggtggg cggcgagccg gtgaaatacg
     3061 agatgaacaa ggccgccggc acgctgttcg tcgaccggtt cctgtacacg ccgatgcgct
     3121 atcccgggaa ttatggcttc gtgccccata ccctgtcgga tgacggcgac ccgatcgacg
     3181 tgctggtggc caacacccgg ccgctcgtgc cgggcgccgt gatcaacgtc cgcccggtgg
     3241 gcgtgctgaa aatggaggac gacggcggcg gggacgagaa gatcctcgcg gttccagtgc
     3301 cgaagctgac ccgtcgctac gagcatgtgc acaactacac cgacctgccg cgcatcacgt
     3361 tggagcagat ccagcacttc ttcgagcact acaaggacct ggaacccggc aaatgggtgc
     3421 gggtgatcgg ctggggcgat tcagcggagg cgcaaaacct gatccgcgac gccatcgcac
     3481 gggcgaaggc ctgaggcggc cgccggtcag cgcggcggca ccaccacgct cagccgggcg
     3541 ccgccagaca ggtcggcggt cttgctctgc aggctggcaa tcagcgcctc ggcaccgccc
     3601 cggtccagca ggctgcggaa atcggagcgc tgcaccgcca cccggctgat cgtgccgtcc
     3661 agcaggacgt cggtgatccg ccagcgcggc ccatcctggt gcaccaggta gtcgatccgc
     3721 accgtattgc cgctggcccg gacgatccgg ctggctacca cccggtcggc gccaacgctg
     3781 cgctgttccg gcaggatctc gaacttctgc ccggaatatt cgtcgaaatt tgccacgtag
     3841 ctggcgacgg tgaaatcgct gaacgccttg aacagccgag cccgggcggc atcggcaagg
     3901 cttgcccagt gcaaccccac cgaatgcatc aggatggcag tcaggtcgaa ggcctgggtg
     3961 atcaccggcg ccaactgggc gaagcgggcg gtgaagggct tgctcgcccc ctcacgcatc
     4021 acggcctcca gcccggcgta cagcgcgctg accggcgcgg ccggtgcgct gtccggctgc
     4081 gccagggccg gaaatggcat agcggccagc agggccaggt gaaccagcgt gcggcgcgaa
     4141 caagcgaccg cccgccgagg cggcagcgag ggcaaaacgg acattccccc tgtataatcg
     4201 ttcagcggcc cggcgaaagt acccgccgtt cagcgcgccg cctcacgcag cacatccagc
     4261 ggttgcagca cgccttcgcg ctcgaaatgc cagaaggtcc agccgttaca ggacggcgcg
     4321 ttctgcaccg cggcgccgac cttgtggatc gagccgcgaa acgcgccgga ggccagcgtg
     4381 ccgtctgccg ccaccaccgc ggtgacccgg cgctgccggt cgtacagctt ggtccccggc
     4441 ggcacctggc cggattccac caggctgccg aaggggatac gcggcgcgtc ccgcttggac
     4501 gccgcgacgg cgagcccctc atgcggcgcc ggcgcggtgt gccggatacg gccgatcgcc
     4561 gcctcggcgt aggcgggatg acgctccagc ccgatgaagt gccggtgcag ccgcttggcc
     4621 accacgccgg tggtgccggt gccgaggaac gggtccagca cgatatcccc cgggctggtg
     4681 ctggccagca gcacgcggtg cagcagcgcc tcgggcttct gggtcgggtg cagtttcagc
     4741 ccgtgcgcgt tgcgcagccg ctccggcccg gtgcacagcg ggatgaacca gtcgctgcgc
     4801 atttggatgt cgtcgttgag cgctttcatc gcct
//
LOCUS       DASSZH010000054         2754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1484094, whole genome shotgun sequence.
ACCESSION   DASSZH010000054 DASSZH010000000
VERSION     DASSZH010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484094"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VFN42_01020"
     CDS             complement(<1..592)
                     /locus_tag="VFN42_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HET8995225.1"
                     /translation="MGGTSKPVRYLELVCPAGTPAALRAAIEAGADTVYCGFRDATNA
                     RNYPGLNFDRDEMADGIRFAHGRGCKVLVAINTFPRPDETAVWHRAVDDAARFGADAV
                     ILADIGVMDYARRRHPALRLHLSVQAGASNPLSLDFYRENFGISRAVLPRVLEVQEIA
                     HLVARMQMEAEVFAFGSLGTMAEGRCILSSYLTGKSP"
     gene            complement(592..1257)
                     /locus_tag="VFN42_01025"
     CDS             complement(592..1257)
                     /locus_tag="VFN42_01025"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HET8995226.1"
                     /translation="MTQDLPSPVMLLKAALHGLPPPLLSPALGAVMLAMRRQHAPLFR
                     RLTRLAPATILFDPVDTPHCFLLRIGKGSVRMELATRGQAASARISGKLATLLQLLEG
                     RIDSDTLFFSRAVSVTGDTAVAVGFRNTLDGESISLMNDALRIAGPFAGPVRRAVLAL
                     DRHIDGVRQELTQRYHAMHEAAHDGRDLAAEHDALAAELNTLREKIARTAASSRRRLG
                     QAA"
     gene            1422..>2754
                     /locus_tag="VFN42_01030"
     CDS             1422..>2754
                     /locus_tag="VFN42_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET8995227.1"
                     /translation="MTQIRRYLPLVVLAGLAAGFLVGPLAWDLPAALVALLIGGDILR
                     SLRRGGLGVDMIALLAIVGALAMREHLTAAIIALMVASGGALEGFADARARRELAGLL
                     DRTPRIAHRHTGDAIADIPAEAVRPGDRLLVKPGEIVPVDGTVAGDAATLDEAALTGE
                     PIPVTRAHGDPVRSGVVNAGTPFMLLATASADRSTYAAVVRLVQQAEQESAPMVRLAD
                     RWALAFLPATLALAGGAWLLAGDPQRALAVLVVATPCPLILAAPVALICGISRAARRG
                     IVVKNGGVLERLARVRTVLFDKTGTLTSGTPRVTGVEAVAGQDADEVLRLAASLEQAS
                     QHVVAAAIVSAARGLNQALSLPEQVEEVPGGGLSGLVEGRRVLVGSAGLLDAAGLPPP
                     AEGAAARMAAAAASASWVALDGRIAGVLLLADRIRPEAPRAIRALRAAGLRR"
BASE COUNT          386 a          960 c          987 g          421 t
ORIGIN      
        1 tgggtgactt gccggtgaga taggaggaca ggatgcagcg cccctctgcc atcgtgccga
       61 ggctgccgaa ggcgaacacc tccgcttcca tctgcatgcg tgcgaccagg tgggcaatct
      121 cctgcacctc gagcacgcgc ggcagcacgg cgcggctgat gccgaaattc tcccgataga
      181 aatccagcga cagcggattg gacgcgccgg cttgcaccga caggtgcagg cgcagcgcgg
      241 ggtgcctgcg gcgggcgtag tccatcaccc cgatatccgc caggatcact gcgtccgcgc
      301 cgaaccgcgc ggcgtcgtca acggcgcggt gccagaccgc ggtctcgtct gggcgcggaa
      361 acgtattgat cgccaccagc accttgcagc cgcgcccatg cgcgaagcgg atgccgtccg
      421 ccatctcgtc ccggtcgaaa ttcaggccgg ggtagttccg cgcattggtc gcatcgcgga
      481 agccgcaata caccgtgtcg gcgcccgcct cgatggccgc acgcaaggcg gcgggcgtgc
      541 cggcgggaca gaccagttcc aggtagcgca ctggtttgga agttccgccc atcacgccgc
      601 ctgtcccagc cggcgccggc tgctcgccgc ggtgcgggcg atcttctcgc gcagcgtgtt
      661 cagttcagcg gcaagggcat cgtgctccgc ggccaggtcg cgcccgtcat gcgcggcctc
      721 atgcatcgcg tggtagcgct gcgtcagttc ctgccttacc ccgtcgatat gccggtccag
      781 cgccagcacc gcgcgtcgga ccgggccggc aaacgggccg gcgatgcgca atgcgtcgtt
      841 catcaggctg attgactccc cgtccagcgt gttgcggaag cccaccgcca ccgcggtatc
      901 gccggtgacg ctgaccgcgc gggagaagaa cagcgtgtcg gaatcgatcc gcccctccag
      961 caactgcagc agagtcgcca gcttgccgct gatccgtgcc gatgcggcct gcccacgggt
     1021 ggcgagttcc attcgaaccg accccttgcc gatgcgcagc aggaagcaat gcggcgtatc
     1081 gaccggatcg aacaggatgg tcgcgggcgc cagccgcgtc agccggcgga acagcggtgc
     1141 gtgctgccgc cgcatcgcga gcatcacggc acccagcgcc ggactcagca gcggcggcgg
     1201 cagcccgtgc agcgcagcct tcagcagcat caccggactc ggcagatcct gtgtcatacc
     1261 tggggttcga tcaggaagtg gaacaacaag gcgcagatga gcggtcgcgc ccgatcgcgt
     1321 ccttgatgca gatcaaacag aaccgaaacg tatcaaccgt accggcgaat tgccttgtgc
     1381 tatatccgcg ccgcttgaac ggggccgctc cgatgggcgc gatgacacag atccggcgct
     1441 acctgccgct ggtcgtgctg gccgggcttg ccgccggctt tctggtggga ccgctcgcct
     1501 gggacctccc cgcggcgctg gtcgcgctgc tgatcggcgg cgatattctc cgcagcctgc
     1561 gccgcggcgg cctgggcgtc gatatgatcg ccctgctggc gatcgtcggc gcactggcga
     1621 tgcgcgagca tctcaccgcc gcgatcatcg ccctgatggt tgccagcggc ggggcgctgg
     1681 aaggcttcgc cgatgcccgt gcgcgtcgcg aactcgccgg cctgctggat cgcacgccgc
     1741 gcatcgcgca ccggcatacc ggcgatgcca tcgccgatat cccggcggag gcggtgcgtc
     1801 cgggtgaccg gttgctggtg aagcctggcg agatcgtccc ggtggacggc accgtcgccg
     1861 gcgatgccgc cacgctggat gaggccgcac tgaccggaga gcccattccg gtcacccgtg
     1921 cgcacggtga ccccgtgcgc agtggcgtgg tgaacgcggg cacccccttc atgctgctgg
     1981 ccaccgcaag cgcggatcgc agtacctatg ccgcggtggt gcgcctggtg cagcaggcag
     2041 agcaggaaag cgcgccgatg gtgcgcctgg cggatcgctg ggcgctcgca ttcctgccgg
     2101 ccaccctggc gctggccggc ggcgcctggt tgctggcagg cgatccgcag cgcgcgctgg
     2161 ccgtgctggt ggtggcgaca ccctgtccgc tgatcctggc ggcaccggtg gcgctgatct
     2221 gcggcatctc ccgcgctgcc cgccgcggca tcgtggtgaa gaacggcggc gtgttggagc
     2281 gcttggcgcg ggtgcgcacg gtgctgttcg acaagaccgg cacgctgacc agcggcacgc
     2341 cgcgcgtcac cggggtggag gcggtggccg gccaggatgc cgatgaggtg ctgcgcctgg
     2401 ccgcgtcgct cgaacaggcc tcgcagcacg tggttgccgc cgcgatcgtc tccgcggcac
     2461 gcgggctgaa ccaggcgctg tcgctgccgg agcaggtgga ggaagtcccg ggcggcgggc
     2521 tgagcgggct ggtggaaggc cgccgcgtgc tggtcggcag cgccggcttg ttggacgccg
     2581 cgggtctgcc gcctccggcc gaaggggccg ccgcccgcat ggcggccgcc gcggcatcgg
     2641 cgtcctgggt cgcgctggac gggcggattg ctggcgtcct gctgctggcc gaccgcatcc
     2701 gccccgaggc gccgcgtgcc atccgcgccc tgcgcgccgc cgggctgcgc cggc
//
LOCUS       DASSZH010000055         2800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1552465, whole genome shotgun sequence.
ACCESSION   DASSZH010000055 DASSZH010000000
VERSION     DASSZH010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552465"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..1665)
                     /gene="secD"
                     /locus_tag="VFN42_01035"
     CDS             complement(88..1665)
                     /gene="secD"
                     /locus_tag="VFN42_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HET8995228.1"
                     /translation="MMYFTRLKTAVILGLCLLGAILCIPNIFPAPAPWLPWRTVHLGL
                     DLRGGSYLLMQVDMNAVVKERLNNLLDGVRQALRPHEIFYQKLQAQPDKNRILLQLRN
                     PAQTTAAAAALKPLITPEGQSNTPDLDIITQPNGVITLTLSQVALTERASSAVQQSIE
                     IVRRRVDDTGVVDPQIERQGSDRIVVQLPGISDPDRIKQLLGKTAHMTFHLVDESANA
                     ASGGPPPPGDEFLPMQNNPREKIAVRRRVDVDGADLTDARAGTNSESGEWVVNFSFNA
                     IGTRKFADITRANVNRRFAVVLDNQVITAPVIREPITGGRGQISGNFTASSATDLAVL
                     LRAGALPAPLTVVEERTVGPELGADSIRAGAISLGVGFVLVIAFMATFYGLFGWFANI
                     CLVVNLVLMLAVMSLLQATLTLPGMAGILLTLGMAVDANILINERVREEQRNGRPPLA
                     ALEHGFTRAYTTIFDSNATAFLAHVMLFVFGSGPVKGFAVTITVGIATSLFTAWMLTR
                     LLVSRWYLANRPKLLPV"
     gene            complement(1766..>2800)
                     /locus_tag="VFN42_01040"
     CDS             complement(1766..>2800)
                     /locus_tag="VFN42_01040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET8995229.1"
                     /translation="TILPLQPWSAAGPAAQAAEQAGFDAVMTVELGHDPFTPLAFAAL
                     ATQRVELTPSVAVAFPRSPTVLASQAWDLQANSGGRFVLGLGSQVKGHNERRFGIPWT
                     APAPRMRDYVLALRAIWRCWETRGRLNFESPHYKLTLMTPDFSPEPTGLPMPAVTIAA
                     VGEAMLRVAGQVGDGVRLHPLCSRKYLEEVCMPQISEGMRRSGRSRVHFDVHGGGFIA
                     TGPDEAAVAAAMQQVRRRIGFYGSTRTYLPILALHGLEDLGAKLHRMSVEGRWDAMAA
                     EVPEEAVRIFAACGTYAEIAGAIEQRFGGVADSIDLHFPPGTPVGLQRELLADIRRIP
                     HTFAGFASGG"
BASE COUNT          443 a          969 c          966 g          422 t
ORIGIN      
        1 gatcaacccc atgaaccgcc ccttcatgaa ctggatgcgg gtgccgtcct tgatgatgcg
       61 gaagcgaggc ttgatgaaca tgagggatca gaccggcagc agcttggggc gattggccag
      121 gtaccagcgc gagaccagca ggcgcgtcag catccaggcg gtgaacagcg acgtggcgat
      181 gccgacggtg atcgtcacgg cgaatccctt caccggaccg gagccgaaga cgaacagcat
      241 cacgtgcgcc aggaaggcgg tcgcgttgga gtcgaagatc gtggtgtagg cgcgcgtgaa
      301 accgtgctcc agcgcggcca gtggcggacg gccgttgcgc tgctcctccc gcacccgctc
      361 gttgatcagg atgttggcgt ccaccgccat gccgagcgtc agcaggatgc cggccatgcc
      421 gggcagcgtc agggtggcct gcagcagcga catgaccgcc agcatcagca ccaggttgac
      481 caccaggcag atattcgcga accagccgaa caggccgtag aacgtggcca tgaaggcgat
      541 gaccagcacg aagccgacgc ccagcgagat ggcgccggcg cggatgctgt cggcgccgag
      601 ttccggaccg accgtgcgct cctccaccac ggtcagcggc gcaggcagcg cgccggcgcg
      661 cagcagcaca gcgagatcgg tggcggagct cgcggtgaag ttgccggaaa tctggccgcg
      721 cccgccggtg atcggttcgc ggatcaccgg ggcggtgatc acctggttgt ccagcacgac
      781 agcgaaccgg cggttgacgt tagcccgggt gatatcggcg aatttccggg tgccgatcgc
      841 gttgaagctg aagttcacca cccactcgcc gctttccgaa ttggtgccgg cgcgcgcgtc
      901 ggtcaggtcg gcgccatcca cgtccacccg gcggcgcacc gcgatcttct cccgcggatt
      961 gttctgcatc ggcaggaatt cgtcgcccgg cggcggcgga ccgccggacg cggcattggc
     1021 gctctcgtcc accaggtgga aggtcatgtg cgccgtcttg ccgagcagct gcttgatccg
     1081 gtcggggtcg ctgatgccgg gaagctggac cacgatgcgg tcgctgccct ggcgctcgat
     1141 ctgcggatcg accaccccgg tatcatccac gcgccggcgg acgatctcga tcgactgctg
     1201 caccgcggag ctggcgcgct cggtcagcgc tacctgcgac agggtcagag tgatgacgcc
     1261 gttcggctgg gtgatgatgt cgaggtcggg ggtgttgctc tgcccttcgg gggtgatcag
     1321 cggcttcaat gccgcggcgg cggcggtggt ctgcgccgga ttgcgcaatt gcagcaaaat
     1381 ccggttcttg tccggctggg cttgcagctt ctggtagaag atctcgtgcg gccgtagcgc
     1441 ctggcggaca ccgtccagca ggttgttgag ccgctccttc accaccgcgt tcatgtccac
     1501 ctgcatcagc aggtaggacc cgccgcgcag gtccagcccc agatgcaccg tgcgccaagg
     1561 cagccagggc gccggggcgg ggaagatgtt ggggatgcac aggatggccc ccaacaggca
     1621 caggcccagg atcacggcgg tcttgaggcg agtgaaatac atcatgggcg gaacgcgggt
     1681 ctccgggcgg caaacggcgc cgcccctatg cgctgtgggc catgcggctg tccaggggcg
     1741 aagccgatcc ggcggccggc cgccctcacc cgccgctggc gaatcccgca aaggtgtgcg
     1801 gtatccgcct tatatccgcc agcagctccc gctgcagccc caccggcgtg ccgggcggga
     1861 aatgcaggtc gatcgaatcc gccacaccgc cgaagcgctg ctcgatcgcc ccggcgattt
     1921 cggcataggt gccgcaggcg gcgaagatgc gcaccgcctc ttccggcacc tcggccgcca
     1981 tcgcgtccca ccgtccttcc accgacatgc ggtgcagctt cgcgcccagg tcttccagcc
     2041 cgtgcagcgc caggatcggc agataggtgc gggtggagcc gtagaacccg atccgccgcc
     2101 gcacctgctg catcgccgcg gccaccgccg cctcgtccgg gccggtggcg atgaacccgc
     2161 cgccgtgcac gtcgaaatgc acccggctgc ggccgctgcg ccgcatgccc tcgctgatct
     2221 gcggcatgca gacctcttcc aggtatttgc gcgagcacag cgggtgcagc cgcaccccgt
     2281 cccccacctg cccggcaacg cgcagcatgg cctcgccgac cgcggcgatg gtcaccgccg
     2341 gcatcggcag cccggtgggc tccggcgaga agtccggcgt catcagggtc agcttgtagt
     2401 gcggcgactc gaagttcagc cgcccgcgcg tctcccagca ccgccagatc gcccgcagcg
     2461 ccagcacgta gtcgcgcatg cgcggcgccg gcgcggtcca ggggatgccg aagcggcgct
     2521 cgttgtgtcc cttcacctgg ctgcccagcc ccagcacgaa gcgcccgccg gaattggcct
     2581 gcaggtccca ggcctggctg gccagcacgg tcgggctgcg cgggaacgcc accgccaccg
     2641 agggggtcag ctccacgcgc tgcgtcgcca gcgcggcgaa ggccagcggg gtgaacgggt
     2701 cgtgcccgag ttccaccgtc atcaccgcgt cgaacccggc ctgttccgcc gcctgcgccg
     2761 cgggaccggc ggcgctccac ggctgcagcg gcaggatggt
//
LOCUS       DASSZH010000056         6469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1569510, whole genome shotgun sequence.
ACCESSION   DASSZH010000056 DASSZH010000000
VERSION     DASSZH010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6469
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569510"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="VFN42_01045"
     CDS             <1..1085
                     /locus_tag="VFN42_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucan biosynthesis glucosyltransferase H"
                     /protein_id="HET8995230.1"
                     /translation="YEGAPPSMTDLAIRDRRWCQGNLQHAAVLPARGLHWVSRMHLLT
                     GIGSYLTAPLWLLFLLAGILISIQARFVPPDYFPQGKALFPSWPVIDPVRAMWMFAGT
                     MGLLLVPKLLGGIAVLLHGPDRRGCGGTLRLLVSLVVETVIAGLAAPVVMLTQSVDVA
                     GILLGRDSGWNAQRRDDGGIPFRVVAQRYRRHTLLGLLLGGAAWAVSLSLALWMLPVV
                     LGLALAIPLAVASGQRRLGLALRRMGLLLIPEETQPPAVLARAEALAREPSEAPRSVL
                     GLFDDPALLASHRDMLPPPRRKRLDPIDPTVLVARVKLAEAESLPEALEVMTRPELLA
                     ALGDRECLDRLQALPHSRSPQLQGSP"
     gene            complement(1213..2511)
                     /locus_tag="VFN42_01050"
     CDS             complement(1213..2511)
                     /locus_tag="VFN42_01050"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET8995231.1"
                     /translation="MLRGDGDLLFDHLDPSHGFWQAGLIIARKLRRAQPGGGLMRDTN
                     AIDRVLQDAVRSGAVPGVVAAAADDAGPFYQGAFGRRSLAGDAAMSMDTVFRIASMTK
                     AITTVAAMQMVEQGKLALDQPAGEVVPDLANPQVLEGFSADGQPILRPARRPVTLRAL
                     LTHTSGFVYDTWNANQMRYAQVTGLPAPRTGKLASLRAPLGFDPGERWEYGIGIDWAG
                     RMVEAVSGLDLEQYMQQHILAPLGMRDTTFDPRPDWGDRLVTVHARAADGSLRPMDTP
                     PPPADREFYPGGGGLYSTASDYLRFLRMLLGGGALEGARVLQPETVALMARNHMGPLL
                     VEKLVTMNPGFSNDAEFFPGMAKKWGLSFLINTEDCPGGRSAGSLAWAGINNTYFWLD
                     PTKRLAGVLMTQILPFADAQVLTLLDAFERAVYSFAAARR"
     gene            2476..3207
                     /locus_tag="VFN42_01055"
     CDS             2476..3207
                     /locus_tag="VFN42_01055"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET8995232.1"
                     /translation="MIEQQVTVTTKHGRMPSFAACPDGAGPYPPIIFYMDAPGTREEL
                     RNMARRIAKHGYFCLLPDMYYRLGTVHFDIPRRDDAMSAVIRASMNSLTNALVVDDTA
                     AMIAWFDTQDKAKPGPLGCVGHCMSGQYITTVSARFPHRMAAAASLYGVGIVTNKEDS
                     PHLLLDRIKGELYYAFAEHDQSVPADVPPALKDALARTDVKATVKVFPGTHHGFCFPE
                     RAVYDTLAAEQTWSDIVAMWDRNLK"
     gene            3357..4745
                     /locus_tag="VFN42_01060"
     CDS             3357..4745
                     /locus_tag="VFN42_01060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET8995233.1"
                     /translation="MTFDTGRSSGWRRLIRTSSFRLTLLYAGLFAFSVLVLLAAMLWW
                     GTSFIAREIDTTVANELSEVQSDAARPGSGGMRAVVAAMTTESPGMRYLLETADGHVL
                     AGNMPALPARVGVQTWQPEVDGLKRLLPLQRGVRGRMVRTTNGDYLFVGLNTFELNEM
                     REMVTRAFFWVLVATLLLAVTVGVVISQGLLNRVEAISRTSREIVAGDLGRRIALRGS
                     DDEFDHLAVSLNAMLDRIQALMEGLNQVSSDIAHDLRTPLTRLRQRLELARLRGDSVE
                     TLHHALDAAMTDVDAILETFAALLRIAQLEAGTQPAEFAALDLSLLLEELLEFYRPVA
                     EEQGHAVAGAIEPAVRVRGDAELLTQMFANLIENALHHTPVGTSITVECRRSDNAVLV
                     EVRDTGPGIPTELRKKVFQRFFRLERSRTTPGTGLGLSLVSAVASLHRVSVELADNQP
                     GLIVRLTIPSGG"
     gene            4875..5588
                     /gene="lexA"
                     /locus_tag="VFN42_01065"
     CDS             4875..5588
                     /gene="lexA"
                     /locus_tag="VFN42_01065"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HET8995234.1"
                     /translation="MLTRKQHELLVFIDRHLRQTGFSPSFEEMKEGLNLKSKSGIHRL
                     ISALEERGFLARRHHRARALEVLKLPENMRDLPAAEAETAGAGFSPNVIRGDFSARIS
                     GVRAANDAAAIQLPLYGRIAAGLPIEALRDTSQHIDVPMALLSGGEHYALEVAGDSMV
                     EAGILDGDTVIIRKDDTAENGQIVVALVDENEVTLKRLRRRGNSIALEPCNVRHETRI
                     FPVDRVKVQGKLVGLLRRY"
     gene            complement(5943..>6469)
                     /locus_tag="VFN42_01070"
     CDS             complement(5943..>6469)
                     /locus_tag="VFN42_01070"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HET8995235.1"
                     /translation="HTRVLECVARADGGVTMPDAELLLRVAALNTSYAACIDADRLEA
                     WPDFFTERCLYKITTADNHRRGLAAGVVYADSRAMLQDRVAALRQANIYERQRYRHIV
                     GLPALQDSADGMVTAETSFLVVRTMRDGRMDVFATGAYLDRMAQSDDGTLRFAERVVV
                     CDSQRFDTLLAIPL"
BASE COUNT         1065 a         2131 c         2165 g         1108 t
ORIGIN      
        1 gctatgaggg cgcgccgccc tcgatgaccg acctggcgat ccgcgaccgc cgctggtgcc
       61 agggcaatct gcagcatgcc gccgtgctgc cggcgcgcgg actgcactgg gtcagccgga
      121 tgcatctgct gaccggcatc ggcagctacc tcaccgcgcc gctctggctg ctgttcctgc
      181 tggcgggcat cctgatctcc atccaggcac gtttcgtgcc gcccgactat ttcccgcaag
      241 gcaaggcact gttccccagt tggccggtga tcgatccggt gcgcgccatg tggatgttcg
      301 ccggcacgat gggcctgttg ctggtgccga agctgctggg tggcattgca gtcctgctgc
      361 atggtcccga tcgccgcggt tgcggcggaa cgctgcgcct gcttgtcagc ctggtggtgg
      421 agacggtgat cgccggcctc gccgcgccgg tggtgatgct gacgcaatcg gtggacgtgg
      481 ccggcattct gctcgggcgg gattccggat ggaatgcgca gcgtcgggac gatggcggca
      541 tcccattccg tgtagtcgcg caacgctatc ggcggcacac gctgctgggg ctgctgctgg
      601 gtggcgccgc ctgggcagtt tcgctgtcgc tggccctgtg gatgttgccc gtggtgcttg
      661 ggttggcgct ggcaatcccg ttggcggtgg cgagcggcca gcggcgcctc gggttggcgt
      721 tgcggcgaat gggattgctg ctgatccccg aggagacgca gccgcccgcg gtgctggctc
      781 gagcggaggc gctggcgcgc gagccttcgg aggctccgcg ttccgtgctc ggtctgtttg
      841 atgacccggc attgctggcg tcgcatcgcg acatgctgcc gccgccgcga cgcaagcggc
      901 tcgatccgat cgatccgacc gtgctggtcg cgcgggtgaa gctggccgaa gccgagtcat
      961 tgcccgaggc gctggaggtg atgacgcgcc cggagcttct agcggcgctg ggcgaccgcg
     1021 aatgtctcga ccggttgcag gcgttgccgc attcccgatc cccgcagctt caaggatcgc
     1081 catgagaccg ggcgtcctac cgcacgtgct ccgctgctct cgccggggcg ggcgtgtcac
     1141 gccttgtgcc ggccggccgc cgctgctgta tccgaaccgc gccggcgcga gggcgaacgc
     1201 cggcacctgg ccctaacgcc gcgcggcagc gaaggaatag acggcgcgct cgaaggcatc
     1261 gagcagcgtc agcacctgtg catccgcaaa gggcaggatc tgcgtcatca gcacgccggc
     1321 aaggcgcttc gttgggtcca accagaaata ggtgttgttg atccccgccc aggcgaggct
     1381 gcccgcagac cggccgccgg ggcagtcttc cgtattgatc aggaagctca ggccccactt
     1441 cttcgccatg ccggggaaga actccgcgtc attggagaag cccgggttca tcgtgaccag
     1501 cttttccaca agcagcgggc ccatgtggtt gcgcgccatc agcgcgacag tttccggttg
     1561 cagcacgcgc gcaccttcca gcgccccacc acccagcagc atgcgcagga atcgcaggta
     1621 gtcgctcgcg gtggagtaca gcccgccgcc gcccggatag aattcacggt ccgccggcgg
     1681 cggcggggta tccatcggcc gcagcgagcc atccgccgca cgtgcatgca ccgtcaccag
     1741 ccggtcgccc cagtccggcc gcggatcgaa ggtggtgtcg cgcatcccca gcggggcaag
     1801 gatgtgctgc tgcatgtact gttcgagatc caggccgctg accgcctcca ccatccggcc
     1861 ggcccagtcg atgccgatgc cgtattccca gcgctcgccc ggatcgaatc ccaacggtgc
     1921 ccgcaatgat gccagcttgc cggtacgcgg cgcgggcagc ccggtgacct gcgcatagcg
     1981 catctggttc gcattccagg tgtcgtagac aaaccccgag gtgtgcgtca gcaatgcccg
     2041 cagcgtcacc ggccggcgtg ccggacgcaa gatcggctgg ccatccgcgg aaaacccttc
     2101 caggacctgg ggattcgcca gatcgggtac cacctcaccg gcgggctgat ccagcgccag
     2161 cttgccctgc tccaccatct gcatcgccgc gacggtggtg atcgccttgg tcatcgaggc
     2221 gatgcggaac actgtatcca tcgacatcgc ggcatcaccg gccaggctgc gccggccaaa
     2281 cgcgccttga tagaacgggc cggcgtcatc cgcggcagcg gcgacgacgc ccggcaccgc
     2341 gccggatcgc acggcgtcct gcagaacgcg gtcgattgca ttggtgtccc gcatgagtcc
     2401 gcctcccggt tgcgcgcggc gcagcttgcg cgcgatgatc aggccagctt gccagaaccc
     2461 atgggagggg tcgagatgat cgaacagcag gtcaccgtca ccacgaagca tggccgcatg
     2521 cccagcttcg cggcctgtcc ggatggggca ggcccgtacc cgccgatcat cttctacatg
     2581 gatgcgcccg gcacgcgcga ggaactgcgc aacatggcac gccgtatcgc caaacacggc
     2641 tatttctgcc ttctcccgga catgtactat cggctcggca ccgtgcactt cgacatcccg
     2701 cgccgcgacg atgcgatgtc cgcggtgatc cgcgcgtcga tgaacagcct gaccaatgcg
     2761 ctggtggtgg acgacacggc cgcgatgatc gcctggttcg acacgcagga caaagcgaag
     2821 cccggcccgc tcggctgcgt cggacattgc atgagcggcc agtatatcac gacggtgtcc
     2881 gcccgcttcc cgcaccgcat ggccgccgct gcatccctgt atggcgtggg gatcgtcacc
     2941 aacaaggaag actcgccgca cctgctgctg gatcgcatca aaggcgagct gtactacgca
     3001 ttcgccgagc acgaccagtc cgtgccggcg gatgtgccgc ctgcgctgaa ggacgcgctg
     3061 gcgcgtactg acgtgaaggc gacggtgaag gtcttccccg gcacgcatca cggtttctgc
     3121 tttccggagc gcgcggtcta cgacacgctg gcggcggagc agacctggtc cgacatcgtc
     3181 gccatgtggg atcgcaatct gaaataggcc tgccttcgac ccggccagtc ttcgcgattg
     3241 gtaacttgac gctccgccgg ccgccgcggc gaatccaccc gccaggaggt ggaagattgc
     3301 gcctgttcca gggagaccgg atggccgttg cgaagagtcg cggcgatccc gcagccatga
     3361 cgttcgacac gggccgcagt agcggctggc ggcggctgat acggacctcc agcttccggt
     3421 tgacgctgct ctatgccggg ctgttcgcct tctccgtgct tgtgctgctc gccgccatgc
     3481 tgtggtgggg cacgtcgttc attgcgcgcg agatcgacac gacggtggcg aacgagttga
     3541 gtgaggtgca atccgacgcc gcccggcccg gctccggcgg catgcgcgcg gttgtggcag
     3601 cgatgaccac ggaatccccg ggcatgcgtt atctgctgga gaccgcggac ggacatgtgt
     3661 tggccggcaa catgccggct ttgccggcgc gggtcggcgt gcagacctgg cagcctgaag
     3721 tcgacgggct caagcgtttg ctgccgttgc agcgtggcgt gcgcggacgc atggttcgga
     3781 ccacgaacgg cgattacctg tttgtcgggc tcaacacctt cgagctgaac gagatgcgcg
     3841 agatggtcac ccgcgccttt ttctgggtgc tggtcgcgac cttgctgcta gctgtcaccg
     3901 tcggagtggt gatcagccag gggttgctga accgggtgga ggcaatcagc cgcaccagcc
     3961 gggagatcgt ggccggcgac ctggggcggc gcatcgcgct gcgcggcagc gatgacgaat
     4021 tcgaccatct tgcggtcagc ctgaatgcca tgctggaccg gatccaggcg ctgatggagg
     4081 ggctgaacca ggtatccagc gacatcgcgc acgatctgcg tacgccgctg acaaggctgc
     4141 ggcagcgcct ggaactggcg cgtctgcgcg gcgacagcgt ggagacgctg catcatgcac
     4201 tggatgcggc gatgaccgat gttgacgcca ttctggagac cttcgctgcc ctgttacgca
     4261 ttgcgcagct tgaggccggc acccaaccgg cggagtttgc cgcgctcgac ctcagcctgc
     4321 tgctggaaga gctgctggaa ttctaccgcc cggtggcgga ggaacagggc cacgccgttg
     4381 ccggcgcgat cgaaccggca gtgcgggtac gcggcgatgc ggagctactg acgcagatgt
     4441 tcgccaacct gatcgaaaat gctctgcatc acacgccggt gggcaccagc atcacggtgg
     4501 agtgccggcg gtcggacaat gccgtgctgg tggaagttcg cgataccggc ccgggaattc
     4561 ccacagagct gcgaaaaaag gtgttccaac gctttttccg gctggagcgc agccggacca
     4621 cgccgggaac cgggctgggc ctcagcctgg tttccgcggt ggcgagcctg caccgggtga
     4681 gcgtcgaact ggcggataat cagccgggac tgatcgtccg gctgacgatc ccgagcggcg
     4741 gatgacagcg cggcgggaat cggcagattg tgtttgacgc ggcaatggaa cctctgtaga
     4801 acaccgctgg ttgccggttt gttccgagcg ggattcgccc cgggccggtc acgagacagg
     4861 gaaatacacc cgccatgctc acgcgcaagc agcacgagct gctggtcttc attgaccggc
     4921 atctgcggca gaccggcttc tcccccagct tcgaggagat gaaggagggg ctcaatctca
     4981 aatccaaatc cggaatacac cggctgattt cggcgttgga ggagcgtggt ttcctggcgc
     5041 gccgccacca ccgtgcccgc gcgctggagg tgttgaagct gccggaaaac atgcgtgacc
     5101 tgccggctgc ggaagccgaa acggccggcg ccggcttctc tccgaacgtc atccgcgggg
     5161 atttttccgc ccgcatctcg ggcgtgcgcg cggccaacga tgccgcggcc atccagcttc
     5221 cgttgtatgg gcgcatcgcc gccggcctgc cgatcgaggc gctgcgcgat accagccagc
     5281 acatcgacgt gccgatggcg ctgctcagcg gcggggaaca ctacgccttg gaagtggccg
     5341 gcgactccat ggtggaggca gggatcctcg atggtgatac ggtgatcatc cgcaaggatg
     5401 acacggcgga gaatggccag atcgtcgtcg cgctggtgga cgagaacgag gtcacgctga
     5461 agcgcctgcg ccgccgcggc aactccatcg cactggagcc gtgcaacgtg cgccatgaga
     5521 cgcgcatttt tccggtcgat cgggtgaagg tgcagggaaa gctggtcggc ttgctgcggc
     5581 ggtactagta ctgcgatccg atcgtgtgac acgtcatggc gaaggctcgc catccacgac
     5641 tggctgtggg agagtcccac agcggcttgc agccgtgagt ctcccccaac acggcgtggc
     5701 ggacctgcgg gccgaaatcc aattcgccaa tcgtcatggc cgggcttgac ccggccatct
     5761 acccgagatg atcgggcatt cgggctctgt gcgacccaat cgttccgggg cggatggccg
     5821 ggtcaagccc ggccatgacg gaagcaggat tgtagtacac gctggcaccc ccgccaccac
     5881 gtggaagcct tgcgtccgtg ccgattgatc catcccccgc cgcccgtgac acgaaccgct
     5941 ggtcacagcg ggatcgccaa taacgtgtcg aagcgctggc tgtcgcagac caccacccgc
     6001 tccgcgaagc gcagcgttcc atcatctgac tgcgccatgc ggtccagata ggcgccggtt
     6061 gcgaacacat ccatccggcc gtcgcgcatg gtgcgcacca ccaggaagga ggtttccgcc
     6121 gtaaccatcc cgtccgccga atcctgcaac gcaggcagcc ccacaatgtg gcgatagcgt
     6181 tgccgctcgt agatgttggc ctggcgcaat gccgccaccc ggtcctgcag catggcgcgc
     6241 gaatcggcat acaccacacc ggccgccagg ccgcggcgat gattgtccgc ggtggtgatc
     6301 ttgtacaggc accgttcggt gaagaaatcc ggccacgcct ccagccggtc ggcatcgatg
     6361 cacgccgcat aggaagtgtt cagcgccgcg acccgcagca gcagctccgc gtccggcatc
     6421 gtcacacccc catcagcccg cgccacacat tccagaaccc gcgtatggc
//
LOCUS       DASSZH010000057         3382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1007114, whole genome shotgun sequence.
ACCESSION   DASSZH010000057 DASSZH010000000
VERSION     DASSZH010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007114"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VFN42_01075"
     CDS             <1..498
                     /locus_tag="VFN42_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HET8995236.1"
                     /translation="AEMQHNLHLAAGALRTSPAEVPARLEAQQETIRRLERQVADLQR
                     KLATGGGEAAVEEVNGVKLAARNLGEVPARDLKGLADTIGKQLGSGVVALVSTADGKA
                     SIVVGVSSDLTSRFNAVELVRAASTAVGGKGGGGRPDMAQAGGPDGARADAALAAIRQ
                     AMDAA"
     gene            complement(536..688)
                     /locus_tag="VFN42_01080"
     CDS             complement(536..688)
                     /locus_tag="VFN42_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1674 domain-containing protein"
                     /protein_id="HET8995237.1"
                     /translation="MSEPNDQPAAPAAEPKPAATAPLPKEIGGAPGPEPTRYGDWEHK
                     GRCTDF"
     gene            complement(736..2148)
                     /locus_tag="VFN42_01085"
     CDS             complement(736..2148)
                     /locus_tag="VFN42_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019088547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HET8995238.1"
                     /translation="MKAVNAIRMGGVDVLPLIEGGKGVAISNGISSGNWAATGGVGTF
                     SAVNADSFDELGERIPQIYRGRTRRERHEELVAYAISGALTQARRAHDLAGGKGRVHA
                     NILWEMGAAERIITEVLEQAKGIIHGITCGAGMPYRLSDIAVRFNVYYYPIISSARAF
                     NALWKRAYSKAASLLGGVVYEDPWLAGGHNGLSNSEDPAKPEDPYPRVLALRKLMREF
                     GLGETPIIMAGGVWWLEEWEHWIDNPELGPVAFQFGTRPLLTKESPIGNAWKQRLLTL
                     EEGDVFLNRFSPTGFYSSAVDNGFIHELRERNDRQVAFTSEVLGEHVAEFGVGPRKRI
                     VYLTPADLERARAWEAQGFTEALRTPDSTLIFVTPEKSREILADQAGCMGCLSSCRFS
                     NWAQHEPDFSNGKKADPRSFCIQKTLQDIAHQSDQPEAVERNLMFSGHNAFRFGRDPF
                     YSNGFIPTVKQLVERILTGR"
     gene            complement(2289..>3382)
                     /gene="pnp"
                     /locus_tag="VFN42_01090"
     CDS             complement(2289..>3382)
                     /gene="pnp"
                     /locus_tag="VFN42_01090"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET8995239.1"
                     /translation="FMLHYNFPPYSVGEAGRMGSPGRREIGHGKLAWRAVHPLLPPKE
                     KFPYTLRVVSEITESNGSSSMATVCGSSLALMDAGVPLKRPVAGIAMGLIKEDKGYAV
                     LSDILGDEDHLGDMDFKVAGTEQGVTSLQMDIKITSITPEIMRIALGQARDGRLHILG
                     EMAKALTGARGDVAQTAPRITIINVPKEKIREVIGTGGKVIREIVEATKCKIDIDDDG
                     TIKIAATDAQQAQQAIDWIRGIVAEPEIGVIYTGKVVKTADFGAFVNFLGSRDGLVHI
                     SELQQGRVGKTTDVVNVGDAVKVKVIGFDERGKVKLSMRVVDQATGADITEQVGAKPG
                     RGDRVRGDRGERRDRAPMPEGTDQPVVGD"
BASE COUNT          497 a         1161 c         1147 g          577 t
ORIGIN      
        1 gcggagatgc agcacaatct gcatctcgcc gccggtgcgc tgcgcaccag cccggccgaa
       61 gtgccggcgc ggttggaagc ccagcaggag accatccgcc gcctggaacg ccaggtcgcc
      121 gacttgcagc gcaagctcgc caccggtggc ggcgaagcgg cggtggagga ggtcaacggc
      181 gtgaagctcg cggcccgcaa tctgggagag gtgccggcgc gcgacctgaa ggggctggcc
      241 gacaccatcg gcaagcagct cggctcgggc gtggtggcgc tggtctccac cgcggacggc
      301 aaggcgagca tcgtggtggg cgtgtcgtcg gacctgacca gccggttcaa cgcggtggaa
      361 ctggtccgcg cggcatcgac cgcggtgggc ggcaaaggcg gcggcggccg gccggacatg
      421 gcgcaggccg gcggccccga cggcgcgcgg gcggatgcgg cgctggcggc gatcagacag
      481 gcgatggacg cagcctagcc gcgcccctac cgccaccaag ggcggcggtc agcggctaga
      541 agtcggtgca gcgccccttg tgttcccagt cgccgtagcg ggtgggttcc ggtcccggcg
      601 ccccgccgat ctccttcggc agcggcgccg tcgcggctgg cttcggctct gcggctggag
      661 ccgcgggctg gtcgttcggt tcgctcatgc ggcggagatg ggcagtgccg gtgtgcaagg
      721 caagccgcgc actgcctagc gcccggtcag gatccgctcc accagttgct tcaccgtcgg
      781 gatgaacccg ttcgaataga acgggtcgcg cccgaagcgg aacgcgttgt ggccgctgaa
      841 catcaggttg cgctccaccg cctccggctg atcgctctga tgcgcgatat cctgcagcgt
      901 cttctggatg cagaagctgc gcggatcagc cttcttgccg ttggagaagt ccggctcgtg
      961 ctgcgcccag ttggagaagc ggcaggagct caggcagccc atgcagccgg cctggtccgc
     1021 caggatttcc cgcgatttct ccggggtgac gaagatcagc gtggagtccg gggtacgcaa
     1081 tgcctcggtg aagccttgcg cctcccaggc gcgggcacgc tccaggtcgg ccggggtgag
     1141 atagacgatg cgcttgcgcg ggccgacgcc gaactccgcc acgtgctcgc ccagcacttc
     1201 ggaggtgaat gccacctggc ggtcgttgcg ctcgcgcagc tcgtggatga agccgttatc
     1261 caccgcgctg gaatagaagc cggtcggact gaaccggttg aggaacacgt cgccttcttc
     1321 cagcgtcaac agacgctgct tccaggcatt gccgatcggg ctttccttgg tcagcaacgg
     1381 gcgcgtgccg aactggaagg caaccgggcc gagttccggg ttgtcgatcc agtgctccca
     1441 ctcctccagc caccacacgc cgccggccat gatgatcgga gtctcgccca ggccgaactc
     1501 ccgcatcagc ttgcgcagcg cgagcacccg cggatacggg tcttccggct tggcggggtc
     1561 ttcgctgttg gacaggccgt tatgcccgcc ggccagccac ggatcctcgt acaccacgcc
     1621 gccaagcagg gaggcggcct tggaataggc gcgcttccac agggcgttga aggcacgggc
     1681 ggaggagatg atcgggtagt agtagacgtt gaaccgcacc gctatgtcgg acaggcggta
     1741 cggcatgccg gcaccgcagg tgatgccgtg gatgatgcct ttggcctgtt cgagcacctc
     1801 ggtgatgatg cgttcggcgg cgcccatctc ccacaggatg ttggcgtgca cccgcccctt
     1861 gccgccggcc aggtcgtgcg cccgccgcgc ctgggtcagc gcgccgctga tggcataggc
     1921 caccagctcc tcatgccgct cccggcgggt ccgcccgcga tagatctgcg ggatccgctc
     1981 gccgagctcg tcgaaactgt cggcgttcac tgcgctgaac gttcccacgc cacccgtcgc
     2041 ggcccagttg ccggacgaga tgccgttgga aatggcgaca cccttgccgc cttcgatcag
     2101 cggcaggacg tccacgccgc ccatgcggat cgcgttgact gctttcatgg cctcgtgccc
     2161 ttcgtcaggc cgctttggca acggcccgac ctgccgctcc cagccccctt ggcgttgtca
     2221 ccgttacgac gcctgtcgcc ggatgcaaga tgtttccgcc cgatgacagg cgtcgcccgg
     2281 cgcttcgttc agtcgccgac aaccggctgg tcggtgcctt ccggcatcgg cgcccgatcg
     2341 cggcgttcgc cgcggtcacc ccggacgcga tcgccgcgac ccggcttggc gccgacctgc
     2401 tcggtgatgt ccgccccggt ggcctgatcg accacccgca tggacagctt caccttgccc
     2461 cgctcgtcga agccgatgac cttcaccttc accgcgtcgc cgacattcac cacgtcggtg
     2521 gtcttgccga cgcggccctg ctgcagctcg ctgatatgca ccagcccgtc gcgcgagccg
     2581 aggaagttga cgaaggcgcc gaaatccgcg gtcttcacca ccttgccggt gtagatcacg
     2641 ccgatctccg gctccgccac gatgccgcgg atccagtcga tcgcctgctg cgcctgctgc
     2701 gcgtcggtgg cggcgatctt gatggtgccg tcgtcgtcga tgtcgatctt gcacttggtg
     2761 gcttcgacaa tctcgcggat caccttgccg ccggtgccga tcacctcgcg gatcttctcc
     2821 ttgggcacgt tgatgatggt gatgcgcggc gcggtctgcg ccacgtcgcc gcgggcaccg
     2881 gtcagcgcct tggccatctc gcccaggatg tgcagccgcc cgtcgcgtgc ctggcccagg
     2941 gcgatgcgca tgatctccgg ggtgatcgag gtgatcttga tgtccatctg caggctggtg
     3001 acaccctgct cggtgccggc caccttgaag tccatgtcgc cgaggtggtc ctcgtcgccc
     3061 aggatgtcgg acagcacggc gtagcccttg tcctccttga tcaggcccat ggcgataccc
     3121 gccaccgggc gcttcaacgg cacgccggca tccatcagcg ccagggagga accgcatacc
     3181 gtggccatcg acgaggagcc gttggactcg gtgatctcgg agacgacgcg cagcgtgtag
     3241 gggaacttct ccttgggcgg cagcagcgga tgcaccgccc gccaggccag cttgccgtgg
     3301 ccgatctcac gccggcccgg cgagcccatg cggccggctt cgcccaccga gtacgggggg
     3361 aagttgtagt gcagcatgaa at
//
LOCUS       DASSZH010000058         6600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_376421, whole genome shotgun sequence.
ACCESSION   DASSZH010000058 DASSZH010000000
VERSION     DASSZH010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6600
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376421"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /gene="glcF"
                     /locus_tag="VFN42_01095"
     CDS             <1..1330
                     /gene="glcF"
                     /locus_tag="VFN42_01095"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HET8995240.1"
                     /translation="QTNFTPEQLRDPDTAASNQVLRTCVHCGFCTATCPTFVLLGDEL
                     DSPRGRIYLIKDMLETGRPATQDVVRHVDRCLSCLSCMTTCPSGVNYMHLVDHARTYI
                     EHTYRRPWHERVLREALGRILPRPALFRLALLGARFGRPFAGMVPGTGPLARRVRAML
                     SLAPRTLPAPGAAERPMVHPAQGKRRARVALLAGCAQQVLAPEINAATIRLLTRLGVE
                     VVVAQGSGCCGALNHHIGQHDPAMHFARANIEAWTRELDGDGLDAVVINASGCGTTVK
                     DYGFMFRAEPDPWPAQAERIASRTLDVTEFLTRLGYTPTRESPGLKVAYHSACSLQHG
                     QRITAEPKTLLARAGFTVVEPAEPHLCCGSAGTYNMLQPEIATRLRARKLDNLRATRP
                     DLIAAGNIGCIAQLSGDSVPVVHTVELLDWMAGGPPPAALAEARRQEPAA"
     gene            1327..1887
                     /locus_tag="VFN42_01100"
     CDS             1327..1887
                     /locus_tag="VFN42_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995241.1"
                     /translation="MKPAALLLAAALSAPPALLHAQPRDQRQDSVNSLLQALKQVPNQ
                     AAAVPIEYQLRRLWLDDGSPAVGLLMKRGLRELQAGENSQAIEDFGDAIVLDPKFAEA
                     YHQRARARYHDGDIAGAIVDLEAALQRQPNDFTVLATLSNIAEAQGNWKGAFEAWQKV
                     LQIDPMTAGGQDRLKQLKLKAFGEQT"
     gene            1911..2144
                     /locus_tag="VFN42_01105"
     CDS             1911..2144
                     /locus_tag="VFN42_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995242.1"
                     /translation="MPSSEGPLSQAARHVMEAEQRVARQSRLLHELERDGHSRLATEA
                     RKVLQTLQRTLELSRNHLRLEQEYAGKRPAKPE"
     gene            complement(2276..3421)
                     /locus_tag="VFN42_01110"
     CDS             complement(2276..3421)
                     /locus_tag="VFN42_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HET8995243.1"
                     /translation="MSACIIDAPDAAQAMPAAPADPPFAGGDRRKVESAVRRAWRKVV
                     GRQGSSGGMRLDDAGCDSLRLLQFAMVLERELGEPLPLDLLDTEMRAEDIVAALSGTP
                     EPPQAEDPRPIVFLLPGLDDDEERLARFRVALQSEARFLLLTYPDWPEMMHPGWCLDD
                     LVKSVADQILQQANGAPALLTGYSFGGEVGFEVACRLVALGQPVRWFGILDADITRLP
                     PPPSGGPMARLQRYLREMADDMRQEGPYKSTGLLVAKLGRQVIGLPRMHRHLGWLRWL
                     PPRAEFWFHRRTRSILRIQALWAWQQAGTPHRLHVPVTLFASEDGNGLSPPDRGWAPL
                     CSALTIVPVSGGHHTLFDSPHRYALYARYAEALHAVVRAEGPGSRPG"
     gene            complement(3484..3960)
                     /locus_tag="VFN42_01115"
     CDS             complement(3484..3960)
                     /locus_tag="VFN42_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010514025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HET8995244.1"
                     /translation="MATIRVQQADFDVSAELAALAAGRTDVGGIGCFVGTVRNSAAGQ
                     PIVAMALEHYPGMTERAIARITDEAERRWSLLGCTVIHRVGRLTPGENIVLVLAASAH
                     RQAALDATAFLIDWLKTKAPFWKREEFRDGGGAWVEAREADDAAAARWQAGGTKTQ"
     gene            complement(3963..4874)
                     /gene="moaD"
                     /locus_tag="VFN42_01120"
     CDS             complement(3963..4874)
                     /gene="moaD"
                     /locus_tag="VFN42_01120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HET8995245.1"
                     /translation="MRRIAPLVLLLALGGCGYRTWSDLPFTGGNNPNKPTGSSETMLR
                     VEGKPTRVAPLNPQAGDVWPGPLPPTPTLEDIEKQTSLEPMQEQAVPGSPRSVGTAPA
                     GTARPAPPPQRARGSSTPPAQQTIPGLPPPVVAPPATPPGAAAPPPEPGQAGKAVQTP
                     NGTGVVTGGSKGYHTMTMPNGGSAIVIPNGNGTSTVIKSDGTIETIPLQARSSMPETA
                     KPLTILYFAWLRERVGRSEETLALPTGVRTVAQLIDHLTARDAAHAAAFDSRRTVRCA
                     VNQEFAPVTAELHPGDEVAFFPPVTGG"
     gene            5214..6029
                     /locus_tag="VFN42_01125"
     CDS             5214..6029
                     /locus_tag="VFN42_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8995246.1"
                     /translation="MTAEPSTQFRGPLLRISGIKVRFGGVIALGGVSFDVEYGQICGL
                     IGPNGSGKTTLFNCISGIYRYETGDISLEGHSLTGVPRHRMAGLGIGRTFQNVALFRP
                     LSVRDNILVGAHHLGHSGFLANAVRLPIVHREERDAEQRLNDLLDLLDLRAVASAPAG
                     SLPFGWQKRVELARALIGAPKLLLLDEPAGGLNHSEVDELAQLLQRIRSHFNMSILLV
                     EHHMNLVMRVSDKVVALEFGMKIADGTPEQVRNEPEVIRAYLGDTEAQHASAA"
     gene            6013..>6600
                     /locus_tag="VFN42_01130"
     CDS             6013..>6600
                     /locus_tag="VFN42_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8995247.1"
                     /translation="MPALLETKGLHAAYGETKVLHGIDFAVAEGGVTALLGANGAGKT
                     TTLRAICGIVKTQGEIWFDGARIDGSATESVVRRGVAHVPDGRGTFMELTVEENMRLG
                     AYTRKDREVASDFERMFGYFPRLRERYHQQAGTLSGGEQQMLAIARALLLRPRLLLLD
                     EPSFGLAPLIVQEIFQIMRRIRAEQQMSILLVEQNA"
BASE COUNT         1067 a         2295 c         2236 g         1002 t
ORIGIN      
        1 gcagaccaac ttcaccccgg agcagttgcg cgatccggac accgccgcgt ccaaccaggt
       61 gctgcgcacc tgcgtgcatt gcggcttctg caccgccacc tgccccacct tcgttctgct
      121 cggcgacgag ttggacagcc cacgcggccg catctacctg atcaaggaca tgctggagac
      181 cggccgaccg gcgacgcagg acgtggtgcg ccacgtcgat cgctgcctgt cctgcctgtc
      241 ctgcatgacc acctgcccgt ccggcgtgaa ctacatgcac ctggtggatc acgcgcggac
      301 ctatatcgaa cacacctacc gccgcccctg gcatgaacgg gtgttgcgcg aggcactggg
      361 ccgcatcctg ccgcggccag cactgttccg cctggcgctg ctgggcgccc gtttcggccg
      421 ccccttcgcc ggaatggtgc ctggcacagg ccccctcgcc cgccgcgtcc gcgccatgct
      481 gtccctggca ccgcgcaccc tgcccgcccc cggcgccgcc gaacggccga tggtgcatcc
      541 cgcccagggc aaacgccgcg cccgcgtggc gctgctggcc ggctgcgccc agcaggtgct
      601 ggcgccggag atcaatgccg ccacgatccg cctgctcacc cggctggggg tggaggtggt
      661 ggtcgcgcaa ggttcaggct gctgcggcgc gctgaaccac catatcggcc agcatgatcc
      721 ggccatgcac ttcgcccgcg ccaatatcga agcctggact cgcgaactcg acggcgacgg
      781 cctggacgcc gtggtcatca acgcctccgg ctgcggcacc acggtgaagg attacggctt
      841 catgttccgt gccgaacccg atccctggcc cgcccaggcg gaacgtatcg ccagccgcac
      901 gctggacgtc accgagttcc tcacccgcct cggttacacg ccgacgcggg agtcgcccgg
      961 gctgaaagtg gcctatcatt ccgcctgcag cctccagcac ggccagcgca tcaccgcgga
     1021 gccgaagacg ctgctggcgc gcgccggctt cacggtggtc gagccggccg aaccgcatct
     1081 ctgctgcggg tcggcgggca cctacaacat gctgcagccg gagatcgcca cccggctgcg
     1141 cgcccgcaag ctggacaatc tgcgcgccac ccggccggac ctgatcgccg ccggcaatat
     1201 cggctgcatc gcccagttga gcggcgacag cgtgccggtg gtgcacacgg tggaactgct
     1261 cgactggatg gcgggtgggc cgccaccggc agcgctcgcc gaagcccgga ggcaggagcc
     1321 ggcggcatga agccggccgc cctcctgctg gccgcggcac tcagcgcgcc tcccgcgctg
     1381 ctgcacgccc agccgcgcga ccagcgccag gattcggtga acagcctgct gcaggcgctg
     1441 aagcaggtcc ccaaccaggc ggccgccgta ccgatcgagt accagctccg gcgactctgg
     1501 ctggatgacg gctctcccgc cgtcggcctg ctgatgaagc gcggtctgcg ggaattgcag
     1561 gccggagaga acagccaggc catcgaggat ttcggcgatg cgatcgtgct cgacccgaaa
     1621 ttcgccgagg cctaccacca gcgtgcccgg gcgcggtacc acgacggcga tatcgccggt
     1681 gcgatcgtcg atctggaagc cgcgctgcag cggcagccaa acgacttcac ggttctggca
     1741 accctctcga acatcgccga ggcgcagggc aattggaagg gcgccttcga ggcctggcaa
     1801 aaggtgctgc aaatcgaccc gatgacagcg ggcggacagg accggctgaa gcagctcaag
     1861 ctgaaggcct tcggcgaaca gacctgacct gcccgtcgcg ggaggctttg atgccgtcat
     1921 ccgaaggtcc actgtcccag gcagcacgcc atgtgatgga ggcagagcag cgcgtggcgc
     1981 gccaatcgcg gctgctgcat gagctggaac gggacggaca ttcccgcctg gccacggaag
     2041 cacgcaaggt gctgcaaacc ctgcagcgga cgctggagct gtcgcgcaac cacctgcggc
     2101 tggagcagga atatgccggc aagcgtccag cgaagccgga gtaacgacgg cggatgtaac
     2161 gggtcgcgtg tgacccggtt gcgctcaccg cgcggtagtg gagcgagaag cgcacgaaca
     2221 tgacaatgcc gggcagcgga ccggcgcgcc gcgtgtcatg cgtgctgacc ccgtttcaac
     2281 ccgggcggct gcccggcccc tctgcgcgca ccaccgcgtg cagcgcttcc gcgtagcggg
     2341 catacagggc gtaccgatgc ggcgaatcga acagggtgtg gtgaccgccg ctcaccggca
     2401 cgatggtcag cgcggaacag agtggtgccc agccgcgatc cggaggggac aggccgttgc
     2461 cgtcctccga cgcgaacagc gtgaccggca cgtgcagccg gtgcggcgtc cccgcctgtt
     2521 gccaggccca gagcgcctgg atccgcagga tggagcgggt gcgccggtgg aaccagaact
     2581 cggcccgcgg cggcagccag cgcagccagc cgagatgccg gtgcatgcgc ggcagcccga
     2641 tcacctggcg gcccagcttc gccaccagca gcccggtgct cttgtatggc ccctcctgcc
     2701 gcatatcgtc ggccatttcc cgcaggtaac gttgcagccg ggccatcggg ccgccgctgg
     2761 gcggcggcgg cagtcgggtg atatcggcgt cgagaatgcc gaaccagcgg acaggctggc
     2821 ccagcgcgac gagccggcac gccacctcga accccacttc gccaccaaag gaatacccgg
     2881 tcagcagcgc gggtgcgcca tttgcctgct ggaggatctg atccgccact gacttcacca
     2941 ggtcgtccag gcaccagccc gggtgcatca tctccggcca gtctggatag gtgagcagca
     3001 ggaatcgcgc ctcggattgc aatgcgacgc ggaaccgggc cagccgctcc tcgtcgtcgt
     3061 cgagtcccgg cagcaggaac acgatcgggc gcgggtcctc cgcctgcggc ggctccggcg
     3121 tgccggacag tgccgccacg atgtcctcgg cgcgcatttc cgtatcgagc aggtccagcg
     3181 gcaacggctc tcccagttcg cgttccagca ccatcgcgaa ctgcagcagg cgcaacgagt
     3241 cgcagcctgc gtcgtccagc cgcatgccac cggatgagcc ttgccggccg accactttgc
     3301 gccaggctcg gcgcacggcg gattcgacct tacggcgatc gccgccggca aacggtggat
     3361 cggcaggagc ggcaggcatg gcttgggcag cgtccggtgc gtcgatgatg caggctgaca
     3421 tgcggcaaca catagcccgc caacccggga agttggatca cgatccccgg caagtgccga
     3481 tggtcactga gtttttgttc cgccggcctg ccagcgcgcg gccgcggcat cgtcggcctc
     3541 acgtgcctcc acccacgcgc cgccgccgtc gcggaattcc tcccgcttcc aaaacggcgc
     3601 cttggtcttc agccagtcga tcaggaacgc cgtggcatcc agcgccgcct ggcggtgggc
     3661 ggaggccgcc agcaccagca cgatattctc ccctggggtc agccggccca cccggtggat
     3721 caccgtgcag ccaagcagcg accagcggcg ctccgcctca tcggtgatgc gggcgatggc
     3781 gcgttcggtc atgcccggat aatgctccag cgccatcgcc acgatcggct ggccggcggc
     3841 actgttgcgc actgtgccga cgaagcagcc gatgccgccc acatccgtcc ggccggcggc
     3901 cagcgccgcc agttccgctg agacgtcgaa atccgcctgc tgcacccgga tggtcgccat
     3961 tcctagcccc ccgtcaccgg cgggaagaat gccacctcgt cacccgggtg cagctcggcg
     4021 gtgaccgggg caaattcctg gttcaccgcg cagcgcaccg ttcggcggct gtcgaaggcc
     4081 gcggcatgag ccgcatcccg cgccgtcagg tggtcgatga gctgcgccac ggtgcgcacg
     4141 ccggtgggca gggccagcgt ttcctccgac cggccgaccc gctcgcgcag ccaggcaaaa
     4201 tacagaatgg tcagcggctt ggcggtctca ggcatgctac ttcgtgcttg gagtgggatc
     4261 gtctcgatgg tgccgtcgga cttgataacg gtgctggtgc cgttgccgtt cggaatgacg
     4321 atggcagagc cgccgttcgg catggtcatc gtgtggtagc ccttggaccc gccggtcacc
     4381 acgccggtgc cgttgggggt ctgcacggcc ttgccggcct gccccggctc gggtggcggc
     4441 gccgcggcgc cgggcggcgt cgccgggggc gccaccacgg gcggcggaag gccgggaatc
     4501 gtctgctggg ccggcggggt ggaactgccg cgtgcccgct gcggcggcgg cgccggccga
     4561 gcggtgccgg ccggcgcggt gcccacgctc cgcggggagc ccggcaccgc ctgttcctgc
     4621 atcggctcca ggctggtctg cttctcgata tcctccagcg tgggggtcgg tggcagcggc
     4681 cctggccaca cgtcgccggc ctgcgggttc aacggcgcga cgcgtgtcgg cttgccctcc
     4741 acccgcaaca tcgtctcgct gctgccggtg ggcttgttcg gattgttgcc gccggtaaag
     4801 ggcaggtccg accaggtgcg ataaccgcag ccgccaaggg ccagcagcag aacgagcggg
     4861 gcgatacgcc gcatgggacg gactccacaa agtgcggaag ggcaagtcac tgaccgatca
     4921 ggttttccgg caaacacggg cggcaatctg cccgcactcg ccgcttcgct caagcaccgc
     4981 ggatcaggtc ggcggccaac ctctcgtgct ccgcggaagt ttgaagcggc agggtaaata
     5041 tttggttgcc ttcgtgcgcg ggcagcgcag cagacgtcga ttgacttgcc cggtcagctt
     5101 cgccaccctg gacgcagtct gggataagcc catcaagggg catagccggg aggacgtatg
     5161 gaaagccgcc attgcgcgac atctggtcgc gtgccttttg agtgccgcgc ctgatgacgg
     5221 cagaaccctc gacccaattc cgcgggcccc tgctgcggat atccggcatc aaagttcgct
     5281 ttggcggcgt catcgcgctg ggcggcgtct ccttcgacgt tgaatacggg cagatctgcg
     5341 ggctgatcgg accgaacggg tccggcaaga ccacgctgtt caactgcatc agcggcatct
     5401 atcgctatga gacaggcgat atcagcctgg aaggccattc gctgaccggc gtgccgcggc
     5461 atcgcatggc cgggcttggc atcggccgca ccttccagaa cgtcgcgctg ttccgcccct
     5521 tgtcggtgcg cgacaacatc ctggtgggcg cgcaccacct cggccattcc ggcttcctgg
     5581 ccaacgcggt tcgcttgccc atcgtgcatc gcgaggaacg cgacgccgaa cagcggctga
     5641 acgacctgct ggatctgctg gacctgcggg cggtggcgtc ggcgccggcg ggcagcctgc
     5701 ccttcggctg gcagaagcgg gtggaactcg cgcgtgcgct gattggcgcc ccgaagctgt
     5761 tgctgctcga cgaaccggca ggcggcctga accacagcga ggtggacgaa ctggcgcagt
     5821 tgctgcagcg catccgcagc catttcaaca tgagcatcct gctggtcgag catcacatga
     5881 acctggtgat gcgcgtgtcg gacaaggtgg tggcgctgga attcggcatg aagatcgccg
     5941 acgggacgcc ggagcaggta cgcaacgagc ccgaagtgat ccgggcctat ctcggcgaca
     6001 cggaggcgca gcatgccagc gctgcttgag accaaggggc tgcatgccgc gtatggcgag
     6061 accaaggtgc tgcacggcat cgatttcgcg gttgccgaag gtggcgtgac cgcgctgctg
     6121 ggggcgaacg gcgccggcaa gacgacgacg ctgcgggcga tctgcggcat cgtcaaaacc
     6181 cagggcgaga tctggttcga cggcgcgcgc atcgacggca gcgccaccga atccgtggta
     6241 cggcgcggtg tcgcgcacgt gccggacggc cgcggcacct tcatggagct gacggtcgaa
     6301 gagaacatgc ggctgggcgc ctacacgcgg aaggaccgcg aggtcgccag cgatttcgaa
     6361 cgcatgttcg gctacttccc acgcctgcgc gagcgctacc accagcaggc cggcacattg
     6421 tccggcggcg aacagcagat gctggcgatc gcccgtgccc tgctgctgcg cccgcggctg
     6481 ctgctgctgg acgagccttc cttcggcctg gcaccgctga tcgtgcagga gatcttccag
     6541 atcatgcgcc gcatccgcgc ggaacagcag atgagcatcc tgctggtcga gcagaatgcc
//
LOCUS       DASSZH010000059         2710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_615207, whole genome shotgun sequence.
ACCESSION   DASSZH010000059 DASSZH010000000
VERSION     DASSZH010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615207"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VFN42_01135"
     CDS             <1..545
                     /locus_tag="VFN42_01135"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HET8995248.1"
                     /translation="DKALLVIDGATPKALSPGQTVRGVRVVRVGDGSAVISVGGRQQE
                     LRVGASPVDLAGKGLQPTVLQALPNGQFVTEGRINGKLVRMMVDTGATMVSISREQAD
                     QLGLSLRGGTPIAVHTANGVVRATQVMLGSVRVGTIEQREVPAVIQDSPLPIVLLGMS
                     FLKRLELRHDADQLILIPRY"
     gene            complement(585..1217)
                     /gene="plsY"
                     /locus_tag="VFN42_01140"
     CDS             complement(585..1217)
                     /gene="plsY"
                     /locus_tag="VFN42_01140"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HET8995249.1"
                     /translation="MTTNLAALFAALAAYLLGSIAFAVVVSRAMGLPDPRSFGSGNPG
                     ATNVLRSGSKLAALLTLGLDACKGWLPVWLAYQAAYAGWLDHAAVPLVAVAVFLGHLY
                     PAFFQFKGGKGVATAAGILLAVSPWLGLATIGTWLIVALFTRYSSLAALAAALFAPFY
                     AWFGSGVAWRGDPAMLLAVTVIALLVVWRHRQNMRNLLAGKESRIGSKRK"
     gene            complement(1270..1758)
                     /locus_tag="VFN42_01145"
     CDS             complement(1270..1758)
                     /locus_tag="VFN42_01145"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HET8995250.1"
                     /translation="MQTAHRPTETPATAWLRSRKAEFSLHAYDYVEHGGAAWGAEALG
                     LDVHEVVKTLVMQNEAAKPLVVLMHGDRQVSTKNLARAIGAKSVQPCVPEVAQRHSGY
                     QVGGTSPFALRKAMPVYVEASVLDLERIYINGGRRGLLLGMRPALLTELLQAVAVHCS
                     LP"
     gene            1906..>2710
                     /locus_tag="VFN42_01150"
     CDS             1906..>2710
                     /locus_tag="VFN42_01150"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET8995251.1"
                     /translation="MPTAPNVDLHCHSTVSDGTLAPAALARRASANGVQIWALTDHDE
                     TGGLAEAAAAAEKLDLHFVPGVEFSVSVGHQTVHIVGLGIDFANPVLLAGLAQVRAGR
                     DTRARLMAQALEQHTRVRGAYAGACRYAGNPALISRTHFARFLVEQGVCGNTHEVFGR
                     FLTPGNPGYVEHAWATLQDAVGWIRAAGGHAVIAHPGRYRFTATEEWALFTSFKEMGG
                     AGVEVMTGSHSAADFRKYAGLALEFGLRASRGSDFHSPQESRCDLGGLPP"
BASE COUNT          468 a          938 c          882 g          422 t
ORIGIN      
        1 gcgacaaggc cctgctcgtg atcgacggcg caacccccaa ggctctgagt ccggggcaga
       61 ctgtgcgggg tgtgagggtg gtgcgggtcg gcgacggttc ggcggtcatc agcgtcggcg
      121 gccggcaaca ggaacttcgc gtcggcgcga gcccggtcga tctcgccggc aagggcttgc
      181 agcccacggt gctgcaagcc ctgccgaacg gccagttcgt gaccgagggc cgcatcaacg
      241 gcaaattggt gcgcatgatg gtggacaccg gcgcgaccat ggtctcgatc agccgggagc
      301 aggccgatca acttggcctg tcgctgcgcg ggggcacgcc gatcgccgtg cataccgcga
      361 acggcgtcgt gcgggcaacc caggtcatgc tcggttcggt gcgcgtcggc acgatcgagc
      421 aacgcgaagt ccccgccgtg attcaggact ccccactccc catcgtgctg ttgggcatga
      481 gttttctcaa acgcctggaa ctgcggcacg acgccgatca gctcattctg attccgcgct
      541 actgagcggt cgcgatacag gcttcgcagg cctgcggtcc cgatctactt gcgcttgctg
      601 ccgatgcggc tttccttgcc ggccagcagg ttgcgcatgt tctgccggtg ccgccagacc
      661 accagcagcg cgatcacggt cacggccaac agcatcgcgg ggtcgccacg ccacgccacg
      721 ccgctgccga accaggcgta gaacggcgcg aacaaggccg ccgccaatgc ggctagcgac
      781 gagtaccgcg tgaacagcgc gacgatcagc caggtgccga tcgtggccag ccccaaccac
      841 gggctgaccg ccagcaggat gccggcggcg gtcgccaccc ccttgccgcc cttgaactgg
      901 aaaaaagccg ggtagagatg gccgagaaaa acggcaaccg cgaccagcgg caccgccgcg
      961 tgatcgagcc agccggcata cgccgcctga taggccagcc agaccggcag ccagcccttg
     1021 catgcatcga gtcccagcgt cagcagcgcc gcgagcttgc tgcccgaacg cagcacattg
     1081 gtggcgccag gattgcccga accgaagctg cgcgggtccg gcaggcccat ggcccggctg
     1141 acgaccacgg caaaggcgat ggaccccaga agataagcgg ccagggcggc gaacagtgct
     1201 gcgaggtttg ttgtcatcgg gcgcgattct attgaacctt gcccgcgcga aacgcaggga
     1261 cccgaagcgt cagggcaggg agcaatgcac ggcgacggcc tgaagcagct ccgtcaacag
     1321 cgccggacgc atgcccagca gcagcccgcg gcgcccgccg ttgatgtaga tacgttccag
     1381 atccagcacg ctcgcctcga catacaccgg catggccttg cgcagggcaa agggcgaggt
     1441 gccgcccacc tgatagccgc tatgccgctg cgccacctcg ggcacacagg gctgcacgct
     1501 cttggcgccg atggcacgcg ccaggttctt ggtcgagacc tggcgatccc catgcatcag
     1561 gaccaccagc ggcttggccg cttcgttctg catcaccaaa gtcttgacga cctcatgcac
     1621 atccaacccc agggcctccg cgccccaggc cgctccaccg tgctcgacat agtcgtaggc
     1681 atgcaggctg aattcggcct tccggctgcg cagccaggct gtcgcggggg tttcggtcgg
     1741 gcgatgggcg gtttgcacgg gtttgatccg atgtggagtc gatgaaggcg cggcggcgac
     1801 gtcacggcat ccgatccggg gctattctgc ccaatcgaca tcatgcgcgc cagtgcgata
     1861 gcccatacgc ggcgcccgct ggggacggcg gcctagaatc cagacatgcc cacagcgccc
     1921 aacgtcgacc tgcactgcca ctccaccgtg tctgacggaa ccctcgcgcc agccgccctg
     1981 gccaggcgcg ccagcgccaa cggcgtgcag atctgggcgc tcacggacca tgatgaaacc
     2041 gggggactgg ccgaagccgc ggctgccgcg gaaaagctcg acctgcactt cgtgccaggg
     2101 gtggagtttt ccgtttcggt tggtcaccag accgtgcata tcgtcgggct cggcatcgat
     2161 tttgccaatc ccgtcttgct ggccggcctg gcccaggtac gcgccggtcg cgacacccgc
     2221 gcccgcctga tggcccaggc gcttgaacaa catacccgcg tgcgcggcgc ctacgccggg
     2281 gcctgccgct atgccggcaa tccggcgctg atctcccgca cccactttgc ccgcttcctg
     2341 gtcgaacagg gtgtttgcgg caacacgcat gaagtcttcg gccgctttct cacgcccgga
     2401 aatcccggtt acgtggaaca tgcctgggcc acgctgcaag acgcggtggg ctggattcgc
     2461 gctgccggcg gccatgccgt cattgcgcat cccgggcgct accgtttcac cgccaccgag
     2521 gaatgggcgc tgtttaccag cttcaaggag atgggcggcg ccggcgtcga agtgatgacc
     2581 ggcagccaca gtgcggcgga cttccgcaag tacgccggcc tggcgctaga gttcggcctg
     2641 cgcgcctcgc gcggttccga tttccacagc ccgcaggaaa gccgctgcga cttgggcggc
     2701 ctacctcccc
//
LOCUS       DASSZH010000060         4671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_410469, whole genome shotgun sequence.
ACCESSION   DASSZH010000060 DASSZH010000000
VERSION     DASSZH010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410469"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1537)
                     /locus_tag="VFN42_01155"
     CDS             complement(143..1537)
                     /locus_tag="VFN42_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995252.1"
                     /translation="MKKGETPEFTVKWWKGSQPKGLKSAGGLESALKDYETAKGKLES
                     SGEDTDADAASKAMDDIIRAVKAVITEASKTKSDEMTATTDCLKKFDRLYAAERKWIE
                     EHTEASDDNAFGDPEVYHVYLLTMLKRLRSSGEMNFGFVLGKKPEDHRLALHKSKSSK
                     GLANILVKQTGIHQMTWGIARPDEERGGLLVLTLEGRVLPGMAKKAGRMLKKFKPLPF
                     KKLALVVDGKEELEDLEAADVADDDDDDLEMPAGTQQDGRDATALTRELAELAKRIQG
                     VSDAGLKGDLARMATQANALLRSNNLPEAGQRIDELRAALDQAPGNTQPGNGASGGAS
                     TVAYAKSRLAWLAARKKVEGEIDKLRSEIVATYKDDGIADQLESSFRTRVAPVLETLD
                     ESLADKLDEVANTTDATARAELVTEAKAIMQRYQSYLSSETLIADLDDNPFSPLTIQQ
                     TISATLSALEKAVH"
     gene            1637..3439
                     /locus_tag="VFN42_01160"
     CDS             1637..3439
                     /locus_tag="VFN42_01160"
                     /inference="COORDINATES: protein motif:HMM:NF017730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HET8995253.1"
                     /translation="MEYAVNFGRLSRPARPASTGQKFRLALLGDFSGRANAGRLETGE
                     ILAKRKPIKVDVDNLDDVIARMGLKLSLPMDEEGGTVEVEIGAIDDFHPDQLAENLDV
                     FGELRTLRRNLGNRASFDRAAKQVLSWGGEAPLPPPPRRARGTDVGHARLSDFARLIG
                     RPAAPEAEEASADALMRRLVGPFIQPARDSRQDALVARVDEALSAAMRRVLHHPDFQT
                     AEALWRGVEFLVRRIETGAKMEIVLYDVSAEELAADLAASDALEQSGLYGMLVEQPAL
                     DAHQGPLSAVAGFYGFELSPPHADLLGRIAQIAGAARAPFIAGMAPDPLKIPFHEQHP
                     LIKDAFTALQSLPAAAYLGLATPRFLLRMPYGRKTDPIDAFAFEEFTRQGGLGGMLWG
                     HPALIPALLLAETYAQQGAKLKLGAVMGVGDIPYYVYTDAAGEQIALPCTERLYTERQ
                     AVQVSQYRVMPLLSIRGRPEVRLGGFTSLAGSPLAGFWAPVDIKPVPEKAVPAAPAAA
                     PQPEPVAAATEPEPEAGVATEPEAAEAPAEDLDALLNSLPEPAAPPAAPAAPAEEPAA
                     DDLDALLASLNAEPEPGAADETEPDLDALLASLK"
     gene            3589..4353
                     /locus_tag="VFN42_01165"
     CDS             3589..4353
                     /locus_tag="VFN42_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995254.1"
                     /translation="MEHVLGVVIPGDPGLAFRQRLDALTPRIRAAAGTPRGEDAKLKA
                     SEAGVFARKKDFERANALLDEAARLVGGTTRGIPPAPPPPPPRSTSQTTGGIPAPPPP
                     PPSTSRGIPPAPPPPSSRGIPPAPPPPPGGKQPDLRAIWRDAKESTDASLEALARELR
                     SYDDADLERIADFGLFGIGRGENVALNKALIEFATARPESRDAAAESLRGAVTAYRSV
                     ISGGLVDYIDNNPFGVSVNVRATLGRALDEIERAVG"
BASE COUNT          723 a         1660 c         1483 g          805 t
ORIGIN      
        1 atccattgga tgtcacgcat cgcccaggcc ggtcagggct gggcgactag catctccgcc
       61 ggaaacggtc gattgctttg ttgccggctc gacattcccg cgcggccacc ggaagccccg
      121 gtggccgttt cgccggcagg aatcagtgta ccgccttctc cagcgccgac agcgtcgccg
      181 aaatggtctg ctggatcgtc agcggcgaga aaggattgtc gtccagatcg gcgatcagtg
      241 tttcgctgct cagatagctc tggtagcgct gcatgatcgc cttcgcctcg gtgaccagct
      301 cggcgcgcgc cgtggcatcg gtggtgttgg cgacctcgtc cagcttgtcg gccaggctct
      361 cgtccagcgt ctccagcacc ggcgcgacgc gcgtgcggaa gctcgactcc aactggtcgg
      421 cgatgccgtc gtccttgtag gtcgccacga tctcgctgcg cagcttgtcg atctcgcctt
      481 ccaccttctt gcgcgcagcc agccaggcca ggcgcgactt ggcataggcc accgtgctgg
      541 caccgccgct cgcgccgttg cccggctggg tgttgcctgg cgcctgatcc agcgccgcgc
      601 gcaactcgtc gatccgctgg ccggcctccg gcaggttgtt gctgcgcagc agggcattcg
      661 cctgcgtcgc catacgcgcc aggtcgcctt tcagcccggc atcgctcacg ccctggatgc
      721 gcttggccag ctccgccagt tcacgggtca gcgccgtggc gtcgcgccca tcctgctggg
      781 tgccggccgg catctccaga tcgtcgtcat cgtcgtcagc gacgtccgcc gcttcgaggt
      841 cttccagctc ctccttgccg tccaccacca aggccagctt cttgaacggc agcggcttga
      901 acttcttcag catccgtccg gccttcttgg ccatgccggg cagcacgcgg ccctccaggg
      961 tcagcaccag caggccgccg cgctcttcgt ccggccgagc gatgccccag gtcatctggt
     1021 ggatgccggt ctgcttcacc aggatattgg cgagcccctt cgacgacttg cttttgtgca
     1081 gggccagccg atggtcctcg ggcttcttgc ccagcacaaa gccgaaattc atctcgccgc
     1141 tgctgcgcag ccgcttcagc atggtgagca gatagacgtg gtagacctcg ggatcgccga
     1201 acgcgttgtc atcgctcgct tcggtgtgct cctcgatcca cttccgctcc gcggcgtaca
     1261 ggcgatcgaa cttcttcagg cagtccgttg tggcggtcat ctcgtcggat ttcgttttgg
     1321 acgcctcggt gatcaccgct ttcaccgccc ggatgatgtc gtccatcgcc ttggaggcgg
     1381 catcggcatc cgtatcctca ccgctggatt ccaacttgcc cttggcggtc tcgtaatcct
     1441 tcagcgcgga ttccagcccg ccggcgctct tcaacccctt cggttggctg cccttccacc
     1501 acttcacggt gaattccggc gtctcaccct tcttcatggc gactctcccc ctcagaccga
     1561 accggcatga ccgcacgcaa cggttggcct ggaatgatgc gctggtatta tctcgaaccc
     1621 gatgcggagt gccagcatgg aatacgccgt caatttcggc cgcctgagcc gtcctgcccg
     1681 tcccgccagc acgggacaga aattccgcct cgccctgctc ggcgattttt ccggacgggc
     1741 caatgccggc cggctggaaa ccggtgagat tttggcaaaa cgcaaaccca tcaaggtgga
     1801 cgtcgataac ctggacgatg tcatcgcccg catggggctc aagctgagcc tgccgatgga
     1861 cgaggaaggc ggcaccgtcg aggtcgagat tggcgcgatc gacgacttcc atcccgacca
     1921 gctggcggaa aacctggatg ttttcggcga actccgcacc ctgcgccgca acctgggtaa
     1981 ccgtgccagt ttcgaccgtg ccgccaagca ggtgctgtcc tggggcggtg aggccccgtt
     2041 gccgccgcca ccccgccgcg cccgcggcac cgatgtgggc cacgcccggc tgtccgactt
     2101 cgcccggctg atcggccgcc ccgccgctcc ggaagccgag gaggcctcgg ccgatgcgct
     2161 gatgcgccgg ctggtcggcc ccttcatcca gccggcacgc gattcccggc aggacgcgct
     2221 ggtcgcccgc gtggacgagg ccctgtccgc cgccatgcgc cgcgtgctgc accatcccga
     2281 cttccagacc gccgaggcgc tgtggcgcgg cgtggagttc ctggtccgcc gcatcgagac
     2341 cggcgcgaag atggaaatcg tgctgtacga cgtatcggcg gaagagctgg ccgccgacct
     2401 cgccgcctcc gacgcgctgg agcagtccgg gctgtacggc atgctggtgg agcagccggc
     2461 gctggatgcg caccagggcc cgctgtcggc cgtggccggt ttctacggct tcgagctgtc
     2521 gccgccgcat gccgacctgc tgggccgcat cgcccagatc gccggcgcgg cgcgtgcgcc
     2581 cttcatcgcc ggcatggcgc cggacccgct gaaaatcccg ttccatgagc agcatccgtt
     2641 gatcaaggat gccttcaccg cgctgcagtc attgccggcg gcggcctatc tcggcctggc
     2701 cacgccgcgc ttcctgctgc gcatgccata cggccgcaag accgacccga tcgacgcctt
     2761 cgccttcgag gagttcaccc ggcagggcgg cctgggtggc atgctgtggg gccatccggc
     2821 gctgatcccc gcgctgctgc tggcggaaac ctacgcccag cagggcgcca agctgaagct
     2881 gggcgcggtg atgggcgtgg gcgacattcc gtactatgtc tataccgacg ccgcgggcga
     2941 acagatcgcg ctgccttgca ccgagcggct ctataccgag cgccaggcgg tgcaggtgag
     3001 tcagtaccgg gtgatgccgc tgctctccat tcgcggccgg ccggaagtgc ggctgggcgg
     3061 gttcacatcg ctggcgggtt cgccgctggc gggattctgg gcgccggtgg atatcaagcc
     3121 ggtaccggag aaggccgtgc cggccgctcc cgcagcggca ccgcaaccgg aacccgtcgc
     3181 cgccgccacg gagccggagc cggaggccgg agtggccacc gaaccagaag ctgccgaagc
     3241 gccggccgaa gatctcgacg cgctgctgaa cagcctgccg gaaccagccg cgccaccggc
     3301 ggcgcccgcg gcaccggcag aggagccggc agccgacgac ctcgacgcgc tactggccag
     3361 cctgaacgcg gaaccggaac cgggcgccgc ggatgagacg gagcccgact tggatgcact
     3421 gctggcatcc ctgaagtgac atctttcatt gccggctgaa cggctgtcat caggaccaca
     3481 catgaaaaca ggtcttcgcc cgcgaagaac gctggaagta cttgcgggcg gaagcgtctc
     3541 catgcttacc cacgcactat gagcggcaca gaccaacaaa aagcctgggt ggagcatgtt
     3601 ctgggagtcg tcatcccagg cgatccgggc ctcgcttttc ggcagcgcct ggatgcgctt
     3661 acgccacgca tccgggccgc ggccggaacc ccacgcggcg aggacgcgaa gctgaaggcc
     3721 agcgaggccg gcgttttcgc ccggaaaaag gatttcgagc gggccaacgc gctgctggat
     3781 gaggccgcca ggctggtggg cgggacgacc cgcggcattc cacccgcgcc gccaccaccc
     3841 ccgccacgtt cgaccagcca gaccacgggc ggcattcccg cgccgccgcc accaccgcca
     3901 tcaacctccc gcggtattcc tcctgcgccg ccaccgccat cctcccgcgg cattcctccc
     3961 gcgccgccgc cgcccccagg gggcaaacag cccgatctgc gggccatctg gcgcgatgcg
     4021 aaggagtcga ccgacgcttc gcttgaggcg ctggcacgcg aactgcgcag ctatgacgat
     4081 gccgacctcg agcggatcgc tgatttcggc ttgttcggca tcggccgtgg ggaaaacgtg
     4141 gcactcaaca aagcgctgat cgagttcgcc acggcgcgcc ccgaaagccg cgacgccgcg
     4201 gccgaaagcc tgcgcggcgc ggtcaccgcc tatcggtcgg tgatcagcgg cggactggtg
     4261 gactatatcg acaacaatcc cttcggcgtt tcggtgaacg tgcgcgccac cttgggccgc
     4321 gcattggacg agatcgaacg cgcggtcgga taacgggcgg caccgccacg ctttcgcggg
     4381 ctccctgttt cacgaagccc tggcaggtct tcgattgggg attgccctcg ccggcctgct
     4441 gggcgaccag ccatccaaac cagaaaccac gatataccga gcagaatgta gagggcgcca
     4501 gaaaaccgcg ggaagctcca cgcggttggg gaaagtggcc gcgcgtcact cggcgcgcac
     4561 gacgatgtcc acccgctggc tctcctgtgc catgcccggc acttcgctca cggtgcgctt
     4621 ctcccgcgtg atacgcggcc gatccacacc gtgctccgcc agtttgtcct c
//
LOCUS       DASSZH010000061         2005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1518553, whole genome shotgun sequence.
ACCESSION   DASSZH010000061 DASSZH010000000
VERSION     DASSZH010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518553"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VFN42_01170"
     CDS             <1..455
                     /locus_tag="VFN42_01170"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HET8995255.1"
                     /translation="DTFEQRLAVRAAASKEPNWPPPIGEFVVSMQNEILLPAPFSPPL
                     EPRQVGPMFEIRQYTLTGGAIPGLIERWSEKIEGRTKFSPLVGAWYSEVGALNKWVHI
                     WAYKDVNERFSVRAAAAATGNWPARNPPGVVIKQENALVMPASFSPIR"
     gene            501..980
                     /locus_tag="VFN42_01175"
     CDS             501..980
                     /locus_tag="VFN42_01175"
                     /inference="COORDINATES: protein motif:HMM:NF013853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 domain-containing protein"
                     /protein_id="HET8995256.1"
                     /translation="MSWSGVSEAEAMSDYDTDLLVWSTQQAALLRRMGAGERVNDQVD
                     WTNLAEEIESLGKSDRRELESRIRTVLVHLIKLLVSPAIEPRVGWQETIVRERAEIQS
                     LVRQSPSLRPTVAQVIVEELPAARKQATIALRGHGAPPVPAEITLSEDQVLGAWLPD"
     gene            1027..1644
                     /locus_tag="VFN42_01180"
     CDS             1027..1644
                     /locus_tag="VFN42_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8995257.1"
                     /translation="MPAAASPRLRIVNLHNRLAGPFDLEVPAGGCVAIAGPSGAGKSL
                     LLRMIADLDPNEGDVFLDGRERRGFTAPEWRRRVAYSAAEPGWWFDTVAPHFPGTALA
                     AARHLAPRLGLAADMLDGPVIRLSTGERQRLALIRAMALESPVLLLDEPTGALDQDST
                     RLVEALLRERLAAGTAMLLVSHSPEQAARLGDRHFRMEARRLVAA"
     gene            1881..>2005
                     /locus_tag="VFN42_01185"
     CDS             1881..>2005
                     /locus_tag="VFN42_01185"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HET8995258.1"
                     /translation="MIRNFSDHAANERTFLAWIRTAIAIMAFGFLVAKFNLFLRIA"
BASE COUNT          319 a          658 c          709 g          319 t
ORIGIN      
        1 atgacacgtt cgagcagcgc ctggccgtgc gggctgcggc gtcgaaggag ccgaactggc
       61 cgccgccgat cggcgagttc gtggtgtcga tgcagaatga gatcctgctg ccggcgccgt
      121 tctccccgcc gctggagccg cgccaggtgg ggccgatgtt cgagatccgg caatacacgc
      181 tgaccggcgg tgcgatcccc ggactgatcg aacgctggtc ggagaagatc gaggggcgca
      241 cgaagttctc cccgctggtc ggtgcctggt attcggaagt cggcgcgctg aacaagtggg
      301 tgcacatctg ggcgtacaag gacgtcaacg agcggttcag cgtgcgtgcc gcggcggcgg
      361 ccacggggaa ctggccggct cgcaatccgc ccggcgtggt gatcaagcag gagaacgcgc
      421 tggtgatgcc ggcgagcttc tcgccgatcc gctgagccgg ccaccgcgac gcagactgga
      481 agccggacca tggaatggac atgtcatggt ccggcgtttc ggaggccgag gcgatgagcg
      541 attacgatac cgatctgctg gtctggagca cgcaacaggc ggcgctgctg cgccgcatgg
      601 gtgccggcga gcgggtgaac gaccaagtgg actggaccaa cctcgcggag gaaatcgaat
      661 cgttgggcaa aagcgatcgg cgcgaactgg aaagccgaat ccggactgtc ctggtgcatc
      721 tgatcaagct gttggtatcg ccggccatag aaccgcgtgt cggttggcag gagacgatcg
      781 ttcgggagcg tgccgaaatc cagagcctgg tcaggcagtc gccgagcctt cgcccaacag
      841 ttgctcaagt catcgtcgag gaactcccgg cggcgagaaa gcaggccacg attgcgttgc
      901 gcggccatgg tgcgccgcca gtcccggccg aaatcactct ctccgaggat caggtgctcg
      961 gcgcctggct gccggattga cgcgatctgc cctggccagc ggacgctgca tgccggacta
     1021 tggcgcatgc ccgccgccgc ttcgccccgc ctgcggatcg tgaatctgca caaccggctc
     1081 gccggcccgt tcgatctcga ggttccggcc ggcggctgcg tcgccatcgc cggcccgtcc
     1141 ggcgcgggca agagcctgct gctgcgcatg atcgccgacc tggacccgaa tgaaggcgac
     1201 gtgttcctgg acggccgcga gcgccgaggc ttcaccgcac cggagtggcg ccggcgcgtg
     1261 gcatacagcg ccgccgaacc cggctggtgg ttcgataccg tcgcgccgca tttccccggc
     1321 accgcgctgg cggccgcccg ccacctggcg ccccgcctgg gcctagcagc ggacatgctg
     1381 gatgggccgg tgatccgcct gtccaccggc gagcgtcagc ggctggcgct gatccgagcc
     1441 atggcgctgg agtccccggt gttgctgctg gacgaaccga ccggggcgtt ggatcaggac
     1501 agcaccaggc tggtggaagc cctgctgcgg gagcgactcg ccgccggtac cgcgatgctg
     1561 ctggtctcgc acagcccgga acaggcggcc cggctcggcg accggcattt ccgcatggaa
     1621 gcacggcggc tggttgcggc atgaccggcc ggcacgcgcc cccccgcgga accgcatgcg
     1681 gagaagtgcc gcgggtcgcc atgcgctgtg attgatctgc ggcgccgccg acgagaaggc
     1741 gcggcgatcc ggagcggagc tgtccggcgc gaggcgcctt gcgtgggcga cccggcctgc
     1801 cgaacgggat ttgattcccg ccggcgcgcg tcactctgcc atgaggtcct catgggcagc
     1861 gatccacggg cgaggctggg atgatccgca atttttccga ccatgccgcc aatgaacgca
     1921 cgttcctggc atggatccgc accgccatcg cgatcatggc tttcggcttt ctggtcgcca
     1981 agttcaacct gttcctgcgc atcgc
//
LOCUS       DASSZH010000062         2649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_683233, whole genome shotgun sequence.
ACCESSION   DASSZH010000062 DASSZH010000000
VERSION     DASSZH010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683233"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VFN42_01190"
     CDS             complement(<1..815)
                     /locus_tag="VFN42_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995259.1"
                     /translation="MLGFLILAGGIGYLWYQQQQFQQHQPDMAAQVQALEQKVQGLNQ
                     QLTALQAQPAHRVPSEIKALSAKVDALQQQKAPDLSRIDSRLAALEQRKPTDLTPLEN
                     QVKALQQQIATTQQEFQQINGLSQQVKTLAQQVQDAATRSQQADSALTQRLDTDESRL
                     VTLEQNAKKISQLTERATRLARLQAAQMALAQGQALGDIPGAPAALARYASDPPPTEA
                     SLRLAFPAAEKAAFNAVSPDTANKPFLSRVLANAEGLVTVRQGDHVLVGNATAG"
     gene            complement(1016..1747)
                     /locus_tag="VFN42_01195"
     CDS             complement(1016..1747)
                     /locus_tag="VFN42_01195"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HET8995260.1"
                     /translation="MPEPPDSTIGSDRDAILITRPEPGATDTAGRVAALGFTPVLCPV
                     LAIRQVAATLPPALRIGAVLVTSRNAIASLPVTYHPVPLLAVGNATADHARQAGFAQV
                     FSADGDAQALSHAVRQQVNPAAGALLLATGRHNGGELAQQMRQAGYRVLRRVVYSAEP
                     RETLEPEAVAALDQGRIRSALFFSAETARHFVNLVRPFPLMASLRAVDAVAIGQAAGM
                     ALEALPWRRVRVAARPNQDEMLALL"
     gene            complement(1740..>2649)
                     /gene="hemC"
                     /locus_tag="VFN42_01200"
     CDS             complement(1740..>2649)
                     /gene="hemC"
                     /locus_tag="VFN42_01200"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003618174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HET8995261.1"
                     /translation="LALHQTRLFLSLLTTFCPVLRGLDVFEEHAISTTGDNVQDRRLA
                     DIGGKGLFAKEIHEALLDGRIDFAVHSLKDLETEMPPGIVLACTLKREDARDALILGP
                     DCGANDPSDPFACLPSGAVIGTASVRRQAQLLHARPDLTVTTIRGNVQTRLAKLAAGE
                     CAASLLALAGLRRLGMAERASVVLDPDAMVPAAGQGIVGITVREADDDLRDLLAGIED
                     PEAKAVSTAERAMLAELDGSCRTPIGGHARLLPNGELHLTGLVARADGSFLLKRSMHG
                     GAQDAARLGAELGASLRADSPRDLFA"
BASE COUNT          384 a          873 c          955 g          437 t
ORIGIN      
        1 ccggcggtgg cgttgccgac cagcacgtgg tcgccctgcc gcacggtgac cagtccctcg
       61 gcattggcca gcacacggga gaggaagggc ttgttggcgg tgtccggcga cacggcgttg
      121 aacgccgcct tttccgccgc cggaaaggcc aggcgcagcg aggcctcggt gggcggcggg
      181 tcggaggcgt agcgcgccaa tgcggcgggg gctcccggaa tgtcacccag cgcctggcct
      241 tgcgccagcg ccatctgggc tgcctgcagc cgggcgagcc gcgtggcacg ctccgtcaac
      301 tggctgattt tcttggcatt ctgctccagc gtgaccaggc gtgattcgtc ggtgtccagc
      361 cgctgggtga gggccgaatc ggcctgctgg ctgcgggtcg cggcgtcctg cacctgttgt
      421 gccagggtct tcacctgctg cgataacccg ttgatctgct gaaattcctg ctgggtcgtc
      481 gcgatctgct gttgcagcgc cttcacctgg ttttccagcg gcgtcaggtc cgtgggcttg
      541 cgctgctcca acgcggcgag gcgggaatca atgcggctca ggtcaggtgc cttttgctgc
      601 tgcaacgcat caaccttagc cgacagtgcc ttgatttcag atggaacccg gtgggccggc
      661 tgtgcctgga gggcggtgag ctgctggttg aggccttgca ccttctgttc cagcgcctgc
      721 acctgtgccg ccatatcggg ctgatgttgc tggaattgct gctgctggta ccagagatag
      781 ccgatgcctc ccgccaggat cagaaacccc agcacgccca gcaccggccc ggcggcagtg
      841 cgacggggcg gcggcgcggc cggggacggc ggttcggggg gcgtctgtgg gtcggcggcg
      901 tgcggtggat cggatgcacc cgtggcgttc ccgtgctggt cgatggtgac caggggatga
      961 tccgtctggt tcggcttcgg ggaatctggg tctggcgggg cgtttggatc agacatcaca
     1021 gcagcgcgag catctcgtcc tggttgggcc gggcggccac gcggacgcgg cgccagggaa
     1081 gggcttctaa tgccatgccg gccgcctgcc cgatagccac tgcatccacc gcgcgcaacg
     1141 acgccatcag cggaaacggc cgtaccaggt tcacaaagtg gcgggccgtt tcggccgaga
     1201 aaaacagggc agagcgaatc cggccctggt ccagcgcagc caccgcctcc ggctccaggg
     1261 tttcgcgcgg ctctgcgctg tagaccaccc gccgcagcac ccggtagccg gcctgccgca
     1321 tctgctgggc cagttcgccg ccattgtgcc ggccggtggc cagcagcagg gctccggcgg
     1381 ccgggttcac ctgctggcgc accgcatggg acagcgcctg cgcgtcgcca tcggcactga
     1441 agacctgggc gaaccctgcc tggcgggcgt gatccgccgt ggcattgccc accgccagca
     1501 gaggcaccgg atgataggtc accggcagcg aggcgatcgc gttcctgctg gtgaccagca
     1561 ccgcgccaat ccgcagcgcc ggcggcagcg tggcggcaac ctggcgaatg gccagcacgg
     1621 ggcacagcac gggggtaaag ccgagcgccg caacgcggcc cgcggtgtcg gttgcaccgg
     1681 gttccggccg ggtgatcagg atcgcgtcgc ggtcggaccc gatggtgctg tcgggcggct
     1741 caggcaaaca ggtcgcgcgg gctgtcggcg cgcaggctgg cgccgagttc agcccccaac
     1801 ctggctgcgt cctgcgcccc accatgcatg gagcgtttca acaggaagga cccgtccgca
     1861 cgcgccacca gtccggtgag gtgcaactcg ccattcggca gcaggcgggc atggccgccg
     1921 atcggtgtgc ggcacgagcc gtccagttct gccagcatgg cgcgttcggc ggtggagacc
     1981 gccttggcct ccggatcctc gatgccggcc agcagatcgc gcaggtcgtc atcggcttcc
     2041 cgcaccgtga tgccgacgat gccctgtccg gcggccggca ccatggcgtc cggatcgagc
     2101 acgaccgacg cgcgttccgc catgcccagc cggcgcaggc cggccaaggc cagcaacgag
     2161 gcggcgcatt cgccggcagc cagttttgcc agccttgtct gcacgttgcc gcggattgtg
     2221 gtgacggtga ggtcggggcg ggcgtgcagc agttgcgcct ggcggcgcac cgaggcggtg
     2281 ccgatcaccg cgccggaggg caggcaggca aacggatcgg acggatcgtt ggcgccgcaa
     2341 tccggaccga ggatcagcgc gtcacgcgca tcctcccgct tcagtgtgca ggcgagcacg
     2401 atgccgggtg gcatctcggt ttcaaggtcc ttcaggctgt gcacggcgaa atcgatccgg
     2461 ccgtcgagca gtgcctcgtg aatttccttg gcgaacaggc ccttgccgcc gatatcggcg
     2521 aggcggcggt cttgcacgtt gtcgccggtg gtgctgatcg catgttcctc gaacacgtcc
     2581 agcccgcgca gcaccgggca gaacgtggtc agcagggaca ggaacaggcg cgtctggtgc
     2641 agcgcgagc
//
LOCUS       DASSZH010000063         3298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1586833, whole genome shotgun sequence.
ACCESSION   DASSZH010000063 DASSZH010000000
VERSION     DASSZH010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586833"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VFN42_01205"
     CDS             complement(<1..738)
                     /locus_tag="VFN42_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET8995262.1"
                     /translation="MIAFAELLERLVFTPARNAKIALLRRYFATQPDPDRGLALAAIA
                     GELAFSAAKPGLIRELAAARTDPVLFAWSYEYVGDLAETVALMWPTKPTGSPPPTLPE
                     VVEALESTPKPRLPDLVGGWLDACDASTRLALLKLITGGLRVGASARLAKIALAEIGG
                     VDPDAVEEVWHGLAPPYLALFAWLEGRGDKPDPADAPVFRPPMLAHPLELADAAALHP
                     EEWRAEWKWDGIRVQLAATPGGRRLYSR"
     gene            890..1858
                     /locus_tag="VFN42_01210"
     CDS             890..1858
                     /locus_tag="VFN42_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET8995263.1"
                     /translation="MNDALPPGAPAPAAVSIEAVEQGLGAAGYIASRQIATAVYLAQH
                     LQKPILVEGPAGVGKTELAKSVAAWLELPLIRMQCYEGLDESKALYEWKYGKQLLYTQ
                     ILKDKIGSVIGETDTLGVALNKLHEFGDIFFSKHFLEPRPLLRALEQPGGSVLLIDEI
                     DKSDQEFEAFLLEILSDYAVTIPEIGPVHAHIPPIVLLTSNSTRDLGDALKRRCLHLH
                     IGFPDEKLEMRIIQSRVPGIEQRLLRQLVSFVQQLRGLDLKKVPSVSETLDWAKVLLL
                     LHTAALEVDLVRDTLNVLLKFEVDIEVAGKQLAGMTHKARQDAGLV"
     gene            1851..3245
                     /locus_tag="VFN42_01215"
     CDS             1851..3245
                     /locus_tag="VFN42_01215"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET8995264.1"
                     /translation="MSESLAPASEPLRRFLQVARGAGLRVSAAEGIDAARAVDIVGFA
                     DRTVLKDTLGLVLAKTPDEKNLFDECFDLYFRRDEFAAPDQQEPQPAVDAAQPPPPMA
                     GSEGMGSGGGAALGDLLLSDDRAALATALEQAARDAGLENIRFFTQKNLYARRILERM
                     GLRGLEREIEAERQTGTPDLIQRADRLQTRLDYLRDQVRDFVERHLVLFARGDTEAFR
                     EELLKSARLSNLERRDLDRMRVLVRAIARKLAARYAKTRRRRLRGQLDTRRTLRRNMG
                     WGGVPFITVWKQKRIEKPRVMVLCDVSGSVAPMAQFLLMFLYAVHEALSDIRSFAFAG
                     SLIEVSDILEKEEIEAAIGRIMQLIGYGSSNYGNSLADFEDGWGAQVTNKTTIIVLGD
                     ARGNRTDPRIEIMQRLSLRAKRIIWLNPEYRSAWGTGDSDMFRYQPFCSLVTVCNTLR
                     HLERVVTDLLEDAT"
BASE COUNT          563 a         1107 c         1097 g          531 t
ORIGIN      
        1 gcgggaatac aggcgccgcc cgccgggagt ggcggccaac tggacgcgga tgccgtccca
       61 tttccactcg gcgcgccatt cctcggggtg cagcgcggcc gcgtcagcca gctccagcgg
      121 gtgcgccagc atgggggggc ggaagaccgg cgcatcggcc gggtccggct tgtcgccacg
      181 gccttccagc caggcgaaca gcgccaggta gggcggggcc aggccgtgcc acacctcctc
      241 caccgcgtcc ggatcgacgc cgccgatctc cgccagggcg atcttcgcca ggcgcgccga
      301 ggcgccgacc cgcagcccgc cggtgatcag cttcagcagc gccaggcgcg tggacgcatc
      361 gcaggcgtcc agccaccccc cgaccaagtc cggcaacctg ggtttcgggg tcgattccag
      421 cgcctccacc acctcgggca gcgttggcgg cggcgagccc gtcggcttgg tgggccacat
      481 cagggcaacg gtctccgcca ggtcgccgac atactcgtac gaccaggcga acagcaccgg
      541 gtcggtgcgg gcggcggcca gttcccggat caggcccggc ttggcggcac tgaaggccaa
      601 ttcgccggcg atggccgcca gggcaaggcc gcggtcgggg tccggctggg tcgcgaaata
      661 gcgccgcagc agcgcgatct tggcgttgcg cgccggggtg aacaccagac gttccaacag
      721 ctccgcgaag gcgatcatgc cgctaatctg ggggttccgc gggcgctgcg ccacacgctg
      781 atccagcgcc cagatccggt gcacgccgcc acgcgaccgg tccgctcgcc ggtttgacat
      841 tccgctgctc ggctgtcagg gaggctactg aacagtagcg gagccagacg tgaacgacgc
      901 gcttccgccg ggggcacccg ccccggccgc tgtgtccatc gaggcggtgg agcagggtct
      961 gggcgccgcc ggttacatcg ccagccgcca gatcgccacc gcggtgtatc tcgcccagca
     1021 tctgcagaag ccgatcctgg tggaaggccc cgctggcgtc ggcaagaccg agcttgccaa
     1081 atcggtcgcc gcctggctgg aactgccgct gatccgcatg cagtgctacg agggcctgga
     1141 cgaatccaaa gcgctgtacg aatggaaata cggcaagcag ctgctctaca cccagatcct
     1201 caaggataag atcggctcgg tcatcggtga aactgacacg cttggcgtgg cactgaacaa
     1261 gctgcatgaa ttcggcgata tcttcttctc caagcatttc ctggaaccgc gcccgctgct
     1321 gcgtgcgctg gaacagcccg gcggatcggt gctgctgatc gacgaaatcg acaagtcgga
     1381 ccaggagttc gaagcgtttc tcctggagat cctgtccgat tacgcggtga ccatcccgga
     1441 aatcggcccg gtgcacgcgc acatcccgcc gatcgtcctg ctcacctcca acagcacgcg
     1501 tgaccttggc gatgcgctga aacgccgctg cctgcacttg catatcggct tcccggacga
     1561 gaagctggaa atgcgcatca tccagtcgcg cgtccccgga atcgagcagc gcctgctgcg
     1621 ccagcttgtc agtttcgtgc agcagcttcg cggcctggat ctgaagaagg tgccctcggt
     1681 cagcgaaacc ctcgactggg cgaaggtgct gctgctgctg cataccgccg ccctggaggt
     1741 ggacctggtg cgcgatacgc tgaatgtgct gttgaagttc gaggtcgata tcgaggtcgc
     1801 cggcaagcag ctcgccggga tgacgcacaa ggcgcggcag gacgcggggc ttgtctgaat
     1861 cgctcgcgcc tgccagcgaa ccgctgcgcc gctttctgca agtggcgcgc ggcgccggtc
     1921 ttcgcgtctc ggcagcggag ggcatcgacg ctgcacgcgc cgtcgatatc gtcgggttcg
     1981 ccgaccgcac ggtattgaag gacacgctcg gtctggtact ggccaagaca ccggacgaga
     2041 agaacctgtt cgacgaatgc ttcgatctct acttccggcg tgacgagttc gccgcgccgg
     2101 accagcagga accgcagcca gcggtggatg cggcgcaacc gcctccaccc atggccggca
     2161 gcgaaggcat gggcagcggc ggtggcgcgg ctctcggcga cctgctgctg tccgacgacc
     2221 gtgcggcgct ggccaccgcg ttggaacagg ccgcgcgtga tgccggcctg gagaacatcc
     2281 gcttcttcac ccagaagaat ctctacgcgc gccgcattct ggagcgcatg gggctgcgcg
     2341 ggctggagcg tgagatcgag gccgagcgtc agaccggcac gccggacctg atccaacgtg
     2401 ccgaccgcct gcagacccgg ctggactacc tgcgcgatca ggtacgcgat ttcgtcgaac
     2461 gccacctggt gctgttcgca cgcggcgata ccgaagcctt ccgcgaagag ctgttgaaat
     2521 ccgcacgcct gtccaacctg gaacgccgcg accttgaccg gatgcgtgtg ctggtgcgcg
     2581 ctatcgccag gaaactggct gcccgctatg cgaagacgcg ccggcggcgg ctgcgcggcc
     2641 agcttgatac gcggcgcacg ctgcgaagga acatgggctg gggcggcgtg cccttcatca
     2701 ccgtgtggaa gcagaagcgg atcgagaagc cgcgcgtgat ggtgctgtgc gacgtctccg
     2761 gctcggtcgc gccgatggcg cagttcctgc tgatgttcct ctacgccgtg cacgaggcac
     2821 tgtccgatat ccgctccttc gccttcgccg gctcgctgat cgaggtcagc gatatcctgg
     2881 agaaggagga gatcgaagcc gccatcggcc ggatcatgca gttgatcggc tacggcagtt
     2941 ccaattacgg caactcgctc gccgatttcg aggatggctg gggcgcgcag gtcaccaaca
     3001 agaccaccat cattgttctc ggcgacgcac gcggcaaccg caccgacccc cggatcgaga
     3061 tcatgcagcg cctgtcgctg cgggcgaagc gcatcatctg gctgaacccg gaatatcgct
     3121 cggcctgggg caccggcgat tccgacatgt tccgctacca gccgttttgc agcctggtga
     3181 cggtttgcaa cacgctccgc cacctggagc gggtggtgac cgacctgctg gaggatgcca
     3241 cctagccggt tgccaagggc ggcggcttac cagcccagga acaggccgat atcgcccg
//
LOCUS       DASSZH010000064         3540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1654884, whole genome shotgun sequence.
ACCESSION   DASSZH010000064 DASSZH010000000
VERSION     DASSZH010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1654884"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VFN42_01220"
     CDS             <1..798
                     /locus_tag="VFN42_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HET8995265.1"
                     /translation="FPGPRTGIAGLRARVRAEGRALTCSALKPQGLSPEQLARLAERF
                     ARGGVDYIKDDHGLADQAYSRFAERVPAIAAAVARAAADTGHPTRYLPSLSGHWEQMR
                     RQIRCARDAGLDTVMLAPMIAGFATVQALTAEFPDMAFFAHPSLGGAARISPPLLIGT
                     LFRLIGADAVIFPTYGGRFGYSPETCRDLATQARAPQPGLRPSLPVPAGGMTLARVPE
                     LLDFYGADAMLLIGGNLLMARDTLTQETAAFTRAVAEHPYRQESHGH"
     gene            788..1246
                     /locus_tag="VFN42_01225"
     CDS             788..1246
                     /locus_tag="VFN42_01225"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8995266.1"
                     /translation="MDTEQTPLLRAFRDGYRWDGVAHQPYKQDGSAPFKDISRQVLFH
                     EEALGCELRYFEMDAGGYSTLERHEHAHAVMILRGQGTCLLGDAVQPVKPFDLVHIPA
                     WTWHQFRATPKEPLGFLCMVNTNRDRPQLPTEQDLAALRAIPAVARFLAG"
     gene            complement(1256..2329)
                     /locus_tag="VFN42_01230"
     CDS             complement(1256..2329)
                     /locus_tag="VFN42_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET8995267.1"
                     /translation="MTDADRVAPETARTQPIARAVLALALMFLGGWTLWEFLPALVWA
                     GIFAIAVWPLYRRVQLRFPPGRHNVLLPALFTLAVALVFLIPLALAGVQLAREVHLAL
                     AWANEARHHGIPPPDWLSNLPFGKAQATAWWNDNLATPEGAADLLSRIGNGDVVATTR
                     HYGSQLLHRLLLFVFTLLTLFFLFRDGDRLVEQLRRVSHRLFGPRGEHLGGQIVASVH
                     GTVDGLVLVGLAEGAVLGVAYVALAVPHAVILGALTALAATIPFGAPLLFGVAALLLL
                     VKGSVGSAIAVTVLGMLVTFVADHFVRPVLIGGATRLPFLWVLLGILGGVASWGLLGL
                     FLGPAIMAALMSVWREWADAEAE"
     gene            complement(2347..3159)
                     /gene="hpnK"
                     /locus_tag="VFN42_01235"
     CDS             complement(2347..3159)
                     /gene="hpnK"
                     /locus_tag="VFN42_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis-associated protein HpnK"
                     /protein_id="HET8995268.1"
                     /translation="MTQQVIFSADDFGLSESVNQAVERAHRDGILDAASLMVAGPAAE
                     DAVRRARALPRLRVGLHLVAIEGPAVLPPHEIPDLVDAAGQFPSDQLRLGVNYFFRPR
                     VRRQLAAEINAQFVAFAATGLKLDHANAHKHMHLHPTVGALMLRIGRQFGLRRVRIPA
                     EPPAVLARCGTPSTFGDRALYRWTGLLRRQALAAGMQVNDHCFGIAWSGHMTADRVRR
                     LVAVLPDGRSEIYFHPAVAQDALLHRLMPDYEHAAEFAALLDHSLRTEHTQP"
     gene            complement(3156..>3540)
                     /locus_tag="VFN42_01240"
     CDS             complement(3156..>3540)
                     /locus_tag="VFN42_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8995269.1"
                     /translation="GGLAVMTAGLLLVLFLPAAATRLDIAWRLALCGVGFGFFQSPNN
                     RVLIASAPPDRAGAGSGMLSTSRLVGQTTGSALVALVFGVTHGTAHAVAAASHICFTI
                     AAGAAGLAMLASTLRLAQSRPGEVA"
BASE COUNT          604 a         1273 c         1191 g          472 t
ORIGIN      
        1 tttcctggcc cgcgcaccgg catcgctggc ctgcgcgcca gggtccgtgc cgagggccgc
       61 gcgctgacct gctcggcgct gaaaccacag ggcctgtcgc ccgagcagct tgccaggctg
      121 gcggaacgct tcgcccgcgg cggcgtggac tacatcaagg acgatcacgg cctggccgac
      181 caggcctaca gccgcttcgc cgaacgcgtc ccggcgatcg ccgccgcagt ggcgcgcgcc
      241 gctgcggata ccggccatcc cacgcgctac ctgcccagcc tgtccggcca ttgggagcag
      301 atgcgcagac agatccgttg cgcccgcgac gccggtctcg ataccgtgat gcttgcgcca
      361 atgatcgccg gcttcgctac ggtccaggcg ctgaccgccg aattccccga tatggcgttc
      421 ttcgcgcatc ccagcctggg cggtgccgca cgcatttcgc cgccactgct gatcggcacg
      481 ctgttccggc tgatcggggc cgatgcggtc atctttccga cctatggcgg ccgtttcggc
      541 tactcgccgg agacctgtcg cgacctcgcc acccaggcgc gcgcgccgca gcccgggctg
      601 cggcccagcc tgccggtgcc ggccggcggc atgaccctgg cgcgtgtgcc ggaactgctt
      661 gacttctacg gcgccgacgc aatgttgctc atcggcggca atctgctgat ggcgcgtgac
      721 actctcacgc aggaaaccgc cgcgttcacc cgcgccgtcg ccgagcatcc gtatcgccag
      781 gaatcgcatg gacactgaac agaccccgct gctgcgcgcc ttccgcgatg gctatcgctg
      841 ggacggcgtg gcgcatcagc cctacaagca ggacggcagc gcgccgttca aggacatctc
      901 ccgccaagtg ctgttccatg aggaagcgct tggatgcgag ctgcgctatt tcgaaatgga
      961 cgccggcggc tactccacgc tggagcggca tgaacacgcc catgccgtaa tgatcctgcg
     1021 cggccagggt acatgcctgc tgggcgacgc ggttcagccg gtgaagccgt tcgacctggt
     1081 gcacatcccg gcatggacct ggcaccagtt ccgcgcgacc ccgaaggagc cgctgggctt
     1141 tctgtgcatg gtcaacacaa accgcgaccg gccgcagctt cccaccgaac aggacctggc
     1201 cgcgctgcgt gccattcccg cggtggcccg ctttctggcg ggctgaccgc cggcatcatt
     1261 ccgcttcggc gtccgcccac tcccgccata ccgacatcaa cgcggccatg atcgcggggc
     1321 cgaggaacag acccagcagg ccccagctcg ccacgccgcc caggatgccc agcagcaccc
     1381 acaggaaggg cagccgcgtg gcgcccccga tcaacaccgg gcggacgaaa tggtcggcga
     1441 cgaaggtgac cagcatgccc agcacggtca ccgcgatggc gctccccacc gagcccttca
     1501 ccagcagcag caaggccgcc acgccgaaca acagcggggc gccgaacggg atggtggcgg
     1561 ccagcgcggt cagcgcgccc aggatcacgg catgcggcac ggcgagcgcc acgtaggcaa
     1621 caccgagcac cgcgccttcc gccaagccga ccagcaccag gccgtccacc gtgccatgca
     1681 ccgacgcgac gatctgcccg cccagatgct cgccgcgcgg gccgaacaga cgatggctga
     1741 cgcggcgcaa ctgctcgacc aggcggtcgc cgtcgcggaa caggaagaac agcgtcagca
     1801 acgtgaagac gaacagaagg aggcggtgca gcaactgcga cccgtaatgc cgggtggtgg
     1861 ccaccacgtc gccgttgccg atgcgggaca gcaggtccgc ggcgccttcc ggcgtggcaa
     1921 ggttgtcatt ccaccaggcg gtggcctgcg ccttgccgaa tggcaggttc gacagccagt
     1981 cgggcggcgg aatgccgtgg tggcgggctt cgttcgccca ggcgagcgcg agatgcacct
     2041 cccgggcaag ctgcaccccg gccagggcca gcgggatcag gaagaccaga gccacggcca
     2101 gggtgaacag cgccggcagc agcacgttgt ggcggccggg cggaaagcgg agttgcaccc
     2161 gccggtatag cggccagacc gcaatggcga agatgccggc ccagaccagg gctggcagga
     2221 actcccacaa ggtccatccg cccaggaaca tcaacgccag cgccagcacg gcgcgggcga
     2281 taggctgggt gcgggcggtt tcgggtgcca cccgatcggc gtctgtcatg tcgctgcgac
     2341 ctggccctat ggctgagtgt gctctgtacg caggctatgg tccaacaatg cggcgaattc
     2401 ggctgcgtgc tcataatccg gcatcagccg gtgcagcaga gcatcctgcg ccaccgcggg
     2461 atggaaatag atctcgctgc ggccatcggg cagcaccgcg accaggcgcc gcacgcggtc
     2521 ggccgtcata tggccgctcc aggcaatgcc gaagcagtgg tcgttgacct gcatgccggc
     2581 tgccagcgcc tggcggcgca gcagcccggt ccagcgatac agcgcccgat cgccgaaggt
     2641 ggacggcgtg ccgcagcggg ccagcacggc gggcggctcc gccgggatcc gcacccgccg
     2701 caagccgaac tgccggccga tccgcagcat cagggccccg acggtgggat gcagatgcat
     2761 gtgcttgtgc gcgttggcgt ggtccagctt caggccggtc gcagcgaacg cgacgaactg
     2821 ggcgttgatc tccgcggcga gttggcgccg gacgcgaggg cggaagaagt agttcacccc
     2881 aagccgcaac tggtcggacg gaaactgccc ggccgcatcg accagatccg gtatctcatg
     2941 cggcggcagc acggccggac cttcgatcgc caccagatgc agcccgaccc gcagccgcgg
     3001 cagtgcccgc gcgcggcgca ccgcatcctc ggctgccggt ccggccacca tcaggctggc
     3061 cgcatccagg atgccgtcgc ggtgggcgcg ctccactgcc tggttgacgc tctccgacag
     3121 gccgaaatca tcggccgaga agatcacctg ctgcgtcatg ccacctcgcc cggacgggac
     3181 tgggccaagc gcagcgtgct ggccagcatg gccagtcccg cggcgccggc ggcaatggtg
     3241 aagcagatat gcgaggccgc cgccacggca tgcgcggtac catgggttac cccgaacacc
     3301 agcgcgacca gcgcgctgcc agtggtctgg ccgaccaggc gggaggtcga caacatgccg
     3361 ctgccggcgc cggcccgatc cggcggcgcg ctggcgatca gcacgcggtt gttcggcgac
     3421 tggaagaagc cgaagcccac accgcacagg gcgaggcgcc aggcgatatc cagccgggtc
     3481 gccgcggccg ggaggaacag caccagcagc agacccgccg tcatcaccgc cagtccgccg
//
LOCUS       DASSZH010000065        13549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_121048, whole genome shotgun sequence.
ACCESSION   DASSZH010000065 DASSZH010000000
VERSION     DASSZH010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 13549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13549
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121048"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VFN42_01245"
     CDS             <1..756
                     /locus_tag="VFN42_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HET8995270.1"
                     /translation="PSKITMLEGRLRNRVRATGSCSLSDYCGLLFDHDGLQEEAVHLI
                     DAVTTNKTDFFREPEHFRILTGRVLPTLLAGDRPGTAQIKLWSAACSIGAEAYTIAMV
                     LADWSQQHGGLRVSILATDLSTEVLAAGQRAVYADSLVSVVPPDMRRRYLLRSRDRAR
                     GLVRIVPELRAWVRFVRLNLMDGEYPVDHDMDVIFCRNVLIYFDKPTQQAVLLRLCGH
                     LRPGGFLFLGHSESLAGMELPLRCIANTVFRRM"
     gene            761..1837
                     /locus_tag="VFN42_01250"
     CDS             761..1837
                     /locus_tag="VFN42_01250"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HET8995271.1"
                     /translation="MKPIKVLIVDDSAVVRQTLAEVLQGDPDIAVLATASDPFVAARR
                     IQAELPDVITLDVEMPRMDGITFLRKLMAQRPIPVVMCSSLTEQGSETLMQALEAGAV
                     DMILKPRIGTAQFLKESKVRICDAVKAAAHARLHRLPATHAARASPTGKLSADVMLPP
                     PGPNHAMARTTERVICIGASTGGTESLRVVLEALPAASPGIVIVQHMPEKFTEAFARR
                     LDGLCELEVKEAADGDAVLRGRALIAPGNRHLLLQRSGARYYVAVKDGPLVSRHRPSV
                     DVLFRSAAHSVGPNGVGIIMTGMGDDGAHGLLEMQQAGALTVAQDEASSVVFGMPKEA
                     IALGAADKVLPLNLMATEIVRAGR"
     gene            1891..2316
                     /locus_tag="VFN42_01255"
     CDS             1891..2316
                     /locus_tag="VFN42_01255"
                     /inference="COORDINATES: protein motif:HMM:NF015908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HET8995272.1"
                     /translation="MISTVLGSCVAVCLWDKVLYAGGMNHFLLPLCKGGPPSPRYGDI
                     AVAMLLENMAALGCRMDNLRAKVFGGAAVLPFGAQETVGTQNLAIALAVLQQHGVPVV
                     ARRTGGQRGLFLRFHTTAGRVMVRELAAQAGSEQEVVRV"
     gene            complement(2290..3933)
                     /locus_tag="VFN42_01260"
     CDS             complement(2290..3933)
                     /locus_tag="VFN42_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995273.1"
                     /translation="MSVAALDQIASRLTPAAKHVEHGFLAAGENLERAVAILDRLNAR
                     FAAHAAELTGGRLDSASEGLDAAACQVTRLADAHGAEAATLGGLSAIVGATIRRVAKL
                     HPITQEIDILALNARVIAAGMGADGRDFLAFTASIQAAVQRARSSLGRVGDDLLQVDR
                     DLRAARTGAEAFALRQEATIRAIPQRLERTNRTLTARRKAAGDAAATARQRAAAISRK
                     VAEQITALQLGDIVRQRLEHMAAGVRLLANQPAGIGLAGRVLAAQLADAASALEAEGQ
                     RIEIRLTQLAANAIAIDQLGMDIQAEAADRQDSFITGLRSDLRQTTALLAELRAADGD
                     AEQRIHAVLDATGILANRLTGVQSVERDIHIIGLNATLKCGRLGADGRALAAVAQELR
                     HCGRRFAGEAAGVLDELGRLRSQVAGLLDPERQNQHAALAQAAETMLAAVEQLHRLED
                     ELRQGMLQLQTDAEQVGRLMADALEQFSVRETVAALMRDSARQIESCGGADGPDTTAL
                     LQRIAATYTMTREREVHARLTPLTLSEAAGASDADDFLF"
     gene            4214..4393
                     /locus_tag="VFN42_01265"
     CDS             4214..4393
                     /locus_tag="VFN42_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995274.1"
                     /translation="MQEKPVQPTNDDPRNESGRISRSGTEAGISIGNESTSGGEGPIP
                     PGGMPKPDKRDDQSR"
     gene            4393..5322
                     /locus_tag="VFN42_01270"
     CDS             4393..5322
                     /locus_tag="VFN42_01270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET8995275.1"
                     /translation="MLPALRTTWLLVRQSVAVFIEDGALSRGAAIAFYAVTSLGPILL
                     IVVAVAGVVYGEQAVRGVLLGKLSAFMGTQSAGFLQSAIGSAWRHGTGTFTTLIGIIS
                     LLVTATGLFSEMQAALNAIWDVSPPATTAWNLVRDRLLGLALVLGLALVLILAVVVSA
                     AINAVQGRVHGLTPFNGFFFQLVNWGGSVIIMMVMVAAVYKVLPDMYLRWRDVAVGAL
                     VTAVLLSLGKMLIGLYIGRTGIASSYGAAGSVIAVMLWIYYSAEIFLLGAEFTSIYAR
                     HRAEVRRSRSESAPPSDRGSVGQHRTSQPERSR"
     gene            complement(5234..6088)
                     /locus_tag="VFN42_01275"
     CDS             complement(5234..6088)
                     /locus_tag="VFN42_01275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET8995276.1"
                     /translation="MHFGAAMFFTDYSMSPAELAQALEQRGFESVWAPEHSHIPTSRR
                     SAWPGGGDLPKQYYDVMDPFVTLAVAAAATKTLKVGTGVCLVNQRDPIQTAKLVASLD
                     QVSAGRFLFGIGVGWNAEEMENHGTEFSSRAKLVRERVEAMKLIWTKSKAEYHGEFVN
                     FTELMTWPKPVQKPHPPVIVGGAFPHGARRAVRYGDGWIPLAGRAQYGDAFDVVPKFR
                     EMLKEAGRDPASCPVSIFNAPEDADTLKRYRELGVQRVSVSVPAAKSDVVLPILDRWA
                     ALIRNVNA"
     gene            6354..7619
                     /locus_tag="VFN42_01280"
     CDS             6354..7619
                     /locus_tag="VFN42_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019088363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET8995277.1"
                     /translation="MSDAIQLTRLPSGLTVVTERMDRVETVSLGAYVATGSRHEEAEE
                     NGVSHFLEHMAFKGTERRTAAQIAEEIEAVGGHINAYTAREQTAYYVKVLKDDTALAA
                     DIIGDILTHSTFEPEELERERGVILQEIGQANDTPDDIIFDHFQEAAYPDHPLGRSVL
                     GSEEGIRNMPRTTLTGYMRRHYQAGNVVVAAAGKLEHGQILELVQQHFADLPGAQAPA
                     MAPGSYHGGEFREERDLDQVHIVLGFPSVAYGQPDYYPTLLLSTLLGGGMSSRLFQEV
                     REKRGLVYSIYSFTAPFLDGGLFGIYAGTGESEAAELMPVTLEELRKVSTGVTEQELA
                     RARAQVKASLLMSLESTGSRSEQLARQLQVFGRVIPTQETVAKLNAVSVEDVCRAAAR
                     HFRATPTLATLGPISRVPMLADIAEKLAA"
     gene            complement(7768..8019)
                     /locus_tag="VFN42_01285"
     CDS             complement(7768..8019)
                     /locus_tag="VFN42_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET8995278.1"
                     /translation="MGEFSIWHWLIVLLVVMLLFGSGKVSNLMGDFAKGIKSFKKNMA
                     EDDHAPTEPPAAEPSASISGPAGDAAPQPAKMRETTPAH"
     gene            complement(8220..8462)
                     /locus_tag="VFN42_01290"
     CDS             complement(8220..8462)
                     /locus_tag="VFN42_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET8995279.1"
                     /translation="MGSLSIWHWLIVLLVVMLLFGSGKVSTLMGDFAKGIKSFKKNMA
                     EDDDASMEASADKPSISGPAGEAAPRAKARETTAAR"
     gene            8859..9068
                     /locus_tag="VFN42_01295"
     CDS             8859..9068
                     /locus_tag="VFN42_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET8995280.1"
                     /translation="MPKGTVKWFNPTKGYGFIAPDTGGKDVFVHISAVQKAGLRSLNE
                     GQKVGFDVEQQQNGRTAAVNLSQTD"
     gene            9222..9977
                     /locus_tag="VFN42_01300"
     CDS             9222..9977
                     /locus_tag="VFN42_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8995281.1"
                     /translation="MQHSFDLSGRVALVTGSSRGLGLAIAQGMAAAGARVILHGRDAA
                     ALRTRAAALPDCAGTAAFDVTDSAGVDAAFARIAEQHGRLDILVNNAGIIPRKPLLET
                     TDKDWQAVIDSNLTAYFRIARESARLMVPRNSGRIIMVSSIMGLVARPTIPGYVTAKA
                     GLHGLVRALAVELAPHGITVNALAPGFFPTDATDVLHKDATFNAWIAGRAPLARWGDP
                     PELAGPAVFLASDAASYVTGHVLVVDGGLSAAL"
     gene            complement(9996..10757)
                     /locus_tag="VFN42_01305"
     CDS             complement(9996..10757)
                     /locus_tag="VFN42_01305"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HET8995282.1"
                     /translation="MNSRAAQVRAPLWAGLAVLVGLCWLWLQQMNAGMPEMAGDMPMA
                     MPHRPLALEVLFATVMWSVMMVAMMVPTALRAIATFASLAARRDASRNVPAAVAVFVG
                     GYGAAWMGYSVLAASLQVVLARTALLTPMLQSASVWLSAAILLAAGMFQFTALKDRCL
                     AKCRTPLAFFLAEWRDGTSGALRLGLRHGGYCVVCCWALMAVLFVAGAMNLLWMAVLT
                     LVLLVEKLAPPKWRISTLSGVALIGWGVWLIGAAV"
     gene            complement(10763..11365)
                     /locus_tag="VFN42_01310"
     CDS             complement(10763..11365)
                     /locus_tag="VFN42_01310"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HET8995283.1"
                     /translation="MTEWKLNGTYFETCNCEAACPCVFTSPPTEGDCTALVAWHIREG
                     SFGETRLDGLNVALAIHAPGTMVETKWKVAAYFDDRASQPQNDALHQIFGGKAGGHPA
                     VLASFIGEILGARSVPMHFEGTGKNASLTIPGIAAAAIEVMTGQGGEPITISNHPLAV
                     APGEPATVARSTQFRFADFGMDWTLSGRNGFLSPFTYMSA"
     gene            11600..11719
                     /locus_tag="VFN42_01315"
                     /pseudo
     CDS             11600..11719
                     /locus_tag="VFN42_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            11735..12925
                     /locus_tag="VFN42_01320"
     CDS             11735..12925
                     /locus_tag="VFN42_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET8995284.1"
                     /translation="MAPRCDGARSAPPPETRHNEDLVWLIRRILEGGDRTVCPGGPWL
                     PNEWLDEASIRQNLNVLARRNGAYLLEYGNPSGYLPLREHFAGTLGEIGVTAQSSQIV
                     LTQGTSQALDLIIRRMLRPGDHVLVDDPGYYNLFGNLRLSDVQLCPVPRLPDGPDMDA
                     LERLAATHQPKLYFTQSVLQNPTGSTMAPHVAFRVLQAAQRYDFRIVEDDIFCDLQAA
                     PTPRLATLDQLQRVIYVRSFSKTLSGSLRVGFAACQQEIADALVEVKMLTSITSSQFA
                     ERLIYLMLVDGHYRKYIARLQKRLADARVNVLRAFEQAGLELFMEPSEGMFLWARSPR
                     VPDSLCLAEKAAADGFVLAPGTVFRPLLEESPWMRFNVAVCDAPNVQRRLETLLNEWV
                     IPTG"
     gene            13170..>13549
                     /locus_tag="VFN42_01325"
     CDS             13170..>13549
                     /locus_tag="VFN42_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010667673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET8995285.1"
                     /translation="MPIPALASPGAMLAMISAALFGASTPVAKFLLGQINPWLLAGVL
                     YLGSGIGLAAVHVGRRSFAIAQPEAPLRRADLPWLAAVVLAGGVIGPLLLMLGLSVTP
                     ASSAALLLNLEGLATMAIAWLVFR"
BASE COUNT         2169 a         4496 c         4559 g         2325 t
ORIGIN      
        1 ccgagcaaga tcaccatgct ggagggcagg ctgcggaatc gggtacgggc gaccggcagc
       61 tgcagcctga gtgactactg tgggctgctg ttcgaccatg acggcctcca ggaggaagcc
      121 gttcacctga tcgacgcggt caccaccaac aagaccgact tcttccgcga gcccgagcat
      181 ttccgcattc tcaccgggcg ggtcctgccc acgctgctgg cgggagaccg ccctggcacc
      241 gcgcagatca aactatggag tgctgcctgc tcgattgggg cggaagccta tacgatcgcc
      301 atggtgctgg cggactggag ccagcagcac ggcgggctgc gggtatcgat tctggcgacc
      361 gatctgtcca ccgaagtcct cgccgccgga cagcgcgccg tctatgcgga ttccctggtc
      421 agcgtggtgc cgccggacat gcgtcggcgc tacctgctgc gcagccgtga cagagcgcgc
      481 ggcctggtgc ggatcgtgcc ggagctgcgc gcctgggtca ggttcgttcg attgaatctc
      541 atggacggcg aatacccggt ggaccacgac atggacgtga tcttctgccg caatgtgctg
      601 atctacttcg ataagccaac gcagcaggcg gtgctgctgc ggctctgcgg acatttgcgc
      661 ccaggcggct tcctgttcct cggccactcc gagtcgctgg ccggaatgga actcccgctg
      721 cgctgcattg ccaacacggt gttccggcgg atgtgagagc atgaaaccga tcaaggtgct
      781 gatcgtggac gactccgcgg tggtacggca gacgcttgcc gaggtgctgc aaggcgaccc
      841 ggacattgcc gtgctggcca ccgcgtccga cccctttgtc gccgcgcggc gcatccaggc
      901 ggaactgccg gacgtcatca cgctggatgt ggaaatgccg cgcatggacg gtatcacctt
      961 cctgcgcaag ttgatggcgc agcggccgat cccggtggtg atgtgttcct cgctcaccga
     1021 gcagggaagc gagacgttga tgcaggcgct ggaagccggc gcggtggaca tgatcctgaa
     1081 accgcgcatc ggcacggcgc agttcctgaa ggaatccaag gtccgcatct gcgatgcggt
     1141 gaaggccgcg gcgcatgccc ggctgcaccg gttgccggca acccacgcag cgcgtgcgtc
     1201 tcccacaggc aaactcagtg ccgatgtgat gctgccgccc cctggcccaa accatgccat
     1261 ggcgcgcacc acggagcggg tgatctgcat cggcgcctcc accggcggca ccgaatcgct
     1321 gcgcgtggtg ctggaggcgc tgcccgcggc cagtcccggc atcgtcatcg tgcagcacat
     1381 gccggagaag ttcaccgaag ccttcgcccg ccgcctggac gggctatgcg agctggaggt
     1441 gaaggaagcc gctgatggcg acgccgttct gcgcggccgg gcactgatcg cgccgggcaa
     1501 ccgtcatctg ctgctgcaac gcagcggcgc gcgttactac gtcgcggtga aggacggacc
     1561 tctggtgtcg cggcatcggc cctcggttga cgtgctgttc cgttccgcgg cgcacagtgt
     1621 cgggccgaat ggcgtcggca tcatcatgac gggcatgggc gatgacggtg cgcacgggtt
     1681 gttggagatg cagcaggccg gcgcgctgac ggttgcgcag gacgaggcca gttccgtggt
     1741 cttcggcatg ccgaaggagg cgatcgccct gggtgcggcg gacaaggtgc tgccgctgaa
     1801 cctgatggcc acggagatcg tgcgggccgg gcggtgatcg tcccagtctt cctgtctccg
     1861 ggccagatcc acgcgtcgcg ggaaccggcg gtgatcagca ctgtgctggg ttcctgcgtg
     1921 gccgtttgcc tgtgggacaa ggtgctctat gccggcggca tgaaccactt cctgctgccg
     1981 ctctgcaaag gcggcccgcc cagcccgcgc tatggcgaca tagcggtcgc gatgctgttg
     2041 gagaacatgg cggcgctggg ctgccgcatg gacaacctgc gcgccaaggt gttcggcggt
     2101 gcggcggtgc tgccgttcgg cgcacaggaa acggtcggca cccagaacct cgccatcgca
     2161 ctcgccgtgt tgcagcagca cggagttccc gtggtcgctc ggcggaccgg cggccaacgc
     2221 gggctgttcc tgcggttcca caccacggcc ggccgggtga tggtgcgcga actggcggcg
     2281 caggccggtt cagaacagga agtcgtccgc gtctgacgcg cctgccgcct cggacagcgt
     2341 cagtggtgtc aggcgtgcat gcacctcgcg ttcgcgggtc atggtgtaag tcgcggcaat
     2401 ccgctggagc agggctgtgg tatccggacc gtcggcaccg ccgcaggatt cgatctgacg
     2461 cgcgctgtcg cgcatcaggg cggccacggt ctcgcggact gaaaactgct ccagcgcgtc
     2521 cgccatcagg cgccccacct gttcggcatc cgtctgaagc tgcagcatgc cctgccgcaa
     2581 ttcgtcttcc agccggtgca gttgctccac cgcggccagc atggtttcgg cggcttgcgc
     2641 cagggccgcg tgctggttct ggcgctccgg atccagcagg ccggccacct gcgaccgcag
     2701 ccggccgagt tcatccagca ccccggctgc ttccccggcg aagcggcggc cgcaatgccg
     2761 caactcctgg gccacggctg ccagggcgcg gccatctgcg ccgagccggc cacatttcag
     2821 cgtggcattc aggccgatga tgtggatgtc ccgctccacg gactgcacgc ccgtcagccg
     2881 gttcgccagg ataccggtgg catccagcac agcgtgaatg cgctgttccg cgtcgccgtc
     2941 ggcggcgcgc aattcggcaa gcaatgcggt ggtctgccgc aggtcgcttc gcaaccccgt
     3001 gatgaagctg tcctgccggt cagcggcttc ggcctggatg tccatgccaa gctggtcgat
     3061 cgcaatggcg tttgccgcga gctgcgtcag gcggatttcg atccgctggc cttccgcttc
     3121 cagcgcgctg gcggcatcgg ccaactgcgc tgccagcacc cggcctgcca ggccgatccc
     3181 ggcaggctgg ttggccagca accggacacc ggccgccatg tgctccagcc gctgccggac
     3241 aatgtcgccc aactggagcg cggtgatctg ttcggcgacc ttgcggctga tcgcggcggc
     3301 ccgctggcgc gcggttgccg ccgcatcccc ggccgccttc cgccgcgcgg tcaacgtgcg
     3361 gttggtgcgc tccagccgct gcgggatggc gcggatggtc gcttcctgcc gcagcgcgaa
     3421 ggcctcggcg ccggtgcgcg cggcgcgcag gtcgcgatcc acctgcagca ggtcgtcgcc
     3481 gacccggccg aggctggagc gggcgcgctg taccgctgcc tggatgctcg cggtgaaggc
     3541 caggaagtcg cggccgtcag cgcccatccc ggccgcgatc acgcgggcgt tcagcgccag
     3601 gatatcgatt tcctgcgtga tggggtgcag ctttgccacc cgcctgatgg tggcgccgac
     3661 gatcgcgctc aaccccccca aggtcgcggc ctccgcgcca tgggcgtcgg ccagccgggt
     3721 cacctggcag gcggccgcat ccagcccctc gctcgcgctg tccagcctgc cgccggtcaa
     3781 ctcggcagca tgcgcggcga agcgtgcgtt cagccggtcc aggatcgcca cggcgcgttc
     3841 caggttttcg ccggccgcca ggaacccatg ctcgacatgc ttggctgccg gggtgagccg
     3901 gctggcgatc tggtcgagcg ctgcaacgga cattgggtcc accattcggc tcccggcata
     3961 gcgcctgtgg cttaaagcaa ggtgaaggcg attgcgggtg tggcgcgttc gccacctgtc
     4021 acagcggcgt gtgacattgg ccggtacatg cccgcgggcg tacgtgccgc gctgccaaag
     4081 ccgcccggcc ggcggaagtg gctgcatgct tcagccggag attcgttgca gccgactgaa
     4141 ccgcctcacg caactgcgcc ggacaggctg gcgttgcgct tcttgctcga tgacagcgca
     4201 aggagacgcc gcgatgcagg aaaaacctgt ccagcccacc aatgacgatc cacgcaacga
     4261 gagcggacgg atcagccgct ccggcaccga ggccgggatt tccattggca atgagagcac
     4321 ctccggtggc gaggggccga tcccgcccgg cggcatgccg aagccagaca agcgggacga
     4381 ccaaagccgc tgatgttgcc ggcgctgcgc acgacgtggc tgctggtcag gcagagcgtc
     4441 gccgtcttca ttgaggacgg tgcgctcagc cgaggggcag ccattgcctt ctatgctgtc
     4501 acctcgcttg ggccgatcct gctgatcgtc gtggcggtgg ccggcgtagt ctatggcgaa
     4561 caggcggtgc gcggcgtgct gctcggcaag ctgtccgcgt tcatggggac ccagagcgcc
     4621 gggttcctgc aaagcgccat cggcagcgcg tggcgccatg gaactggcac cttcacgaca
     4681 ttgatcggaa tcatctcgct gctggtcacc gccaccggcc tgttcagcga aatgcaggcg
     4741 gcgctgaacg cgatctggga tgtctcacca ccggccacga cggcgtggaa tctggtgcgc
     4801 gaccgcctgc ttgggcttgc cctggtgctt ggtctcgcgc tcgtgctgat cctggcggtc
     4861 gtggtttcgg cggcgatcaa tgccgtgcag ggccgcgttc acggcctgac gccgttcaac
     4921 gggttcttct tccaactggt gaactggggg ggatccgtga tcatcatgat ggtcatggtg
     4981 gcggcggtgt acaaggtcct gcccgacatg tatctgcgct ggcgcgacgt cgccgtcggc
     5041 gccctggtga ccgccgtgct gctcagtctc gggaaaatgc tcatcggcct gtatatcggc
     5101 cgcaccggga ttgcctcctc ctacggcgcg gccggttcgg tgatcgcggt catgctgtgg
     5161 atctactatt ccgcggaaat cttcctgctc ggcgcggagt tcaccagcat ctacgcacgt
     5221 caccgggccg aggtcaggcg ttcacgttcc gaatcagcgc cgcccagcga tcgaggatcg
     5281 gtaggacaac atcggacttc gcagccggaa cgctcacgct gacccgctgc acgcccagtt
     5341 cgcggtagcg cttcagcgtg tcggcatctt ccggcgcatt gaagatgctg acgggacagc
     5401 tagcgggatc ccggcctgct tccttcagca tctcgcggaa ctttggcact acgtcgaacg
     5461 cgtcgccgta ctgcgcacgg ccggcaagcg gaatccagcc gtcgccatag cgcaccgccc
     5521 ggcgcgcgcc atgcgggaac gcgccgccga cgatcaccgg cggatgcggc ttctgcaccg
     5581 gtttgggcca ggtcatcagt tcggtgaaat tgacgaactc cccgtggtat tccgccttgc
     5641 tcttggtcca gatcagcttc atggcctcga cccgctcgcg caccagcttc gcgcgggagg
     5701 agaactcggt gccgtggttc tccatctcct cggcgttcca gcccacgccg atgccgaaca
     5761 ggaagcgccc ggcggaaacc tggtcgaggg acgcaaccag cttggcggtc tggatggggt
     5821 cgcgctggtt caccaggcag acgccggtgc cgactttcag ggtcttggtg gccgcggcgg
     5881 cgacggccag cgtcacgaag gggtccatca cgtcatagta ctgcttgggc agatcgccgc
     5941 cgcctggcca cgcagaccgg cgtgaggtgg ggatatgcga gtgctccggc gcccaaacgg
     6001 actcgaagcc acgctgctcc agtgcctgcg ccagttcggc cggagacatg gaatagtcgg
     6061 tgaagaacat cgcggcgccg aagtgcatga caggacctcc cggttatccg gcaagtatag
     6121 gccggttcct tgcggcccgg ctatgcagcg cgcccagccg gcgtgccgcc attgaatccg
     6181 ccccgatcgg gcgcaggaat ttcgtgggat ggcgcggcgg gtggccgtcc tggcgcaact
     6241 gttcaatgca gtggctggaa gatatcatcc gcggcccacc cagggctgac gcggaccgct
     6301 tccgccccct atatctgtcc gacaccgacg ccctatccag gaaacccagc ccagtgtccg
     6361 atgccatcca gctcacgcgc ctgccctccg gcctgaccgt cgtcaccgag cgcatggacc
     6421 gcgtggagac cgtctccctt ggcgcctatg tcgccaccgg ctcgcgccac gaggaggcgg
     6481 aggagaacgg cgtctcccat ttcctggaac acatggcctt caagggtacc gaacggcgca
     6541 cggcagcgca gatcgctgag gagatcgagg cggtcggcgg ccacatcaac gcctacaccg
     6601 cacgggaaca gaccgcctac tacgtcaagg tgctgaaaga cgatactgca ctggccgccg
     6661 acatcatcgg cgacatcctg acccactcca ccttcgagcc ggaggagctg gagcgcgagc
     6721 gcggggtcat cctgcaggag atcggccagg ccaacgacac gccggacgac atcatcttcg
     6781 accatttcca ggaagccgcc tatcccgacc acccgctcgg ccgttccgtg ctgggatcgg
     6841 aggagggcat ccgcaacatg ccccgcacca cgctgaccgg ctatatgcgg cggcattacc
     6901 aggccggcaa cgtggtggtg gccgctgccg gcaagctgga gcatggccag atcctggaac
     6961 tggtgcagca gcatttcgcc gacctgccgg gcgcgcaggc gccggcaatg gcgccgggca
     7021 gctaccatgg cggcgagttc cgcgaggaac gcgacctgga tcaggtgcac atcgtgctgg
     7081 gctttccctc agtggcttac gggcagccgg actattaccc gacactgttg ctctccacat
     7141 tacttggtgg cggcatgtcg tcccggctgt tccaggaagt gcgggagaag cgcggcctgg
     7201 tctactcgat ctattccttc accgcgccgt tcctggatgg cggtttgttc ggcatttatg
     7261 ccggcaccgg cgagagcgag gccgcggaac tgatgccggt gacgctggag gaattgcgca
     7321 aggtcagcac cggcgtgacc gagcaggagc tggcgcgggc gcgggcgcag gtgaaggcgt
     7381 cgctgctgat gtcgctggag agcaccggca gccgttcgga gcagcttgcg cggcagcttc
     7441 aggtcttcgg ccgggtgatc ccgacccagg agacggtggc caagctgaac gcggtgagcg
     7501 tggaggatgt ctgccgagcc gcggcgcggc atttccgcgc gacgccgacc ctggcaacgc
     7561 tggggccgat cagccgcgtg ccgatgctgg cggatatcgc ggagaagctg gccgcataag
     7621 ggcggacggg gaagatagga caagtagaac gcccctcccc gtgcgggagg ggctggggga
     7681 gggggcgatc gaggttcccc ggggagcgca tgccggcccc ctcccgcaag gtgaggggga
     7741 gtgtttctac tcccccgctc cgcgcattca gtgcgccgga gtggtttccc gcatcttggc
     7801 cggctgcggt gcagcatcgc cggccggccc ggaaatcgag gcgctgggct ccgccgcggg
     7861 cggttccgtc ggggcgtggt cgtcctctgc catgttcttc ttgaaggact tgatgccctt
     7921 cgcgaagtca cccatcaggt tgctgacctt gccgctgcca aacagcagca tcacgacgag
     7981 caacacgatc agccaatgcc aaatgctgaa ttcacccatg aggcgtcctt ccaggggctc
     8041 tgccggtctg acatgaagac aggccgggct ttgcacgttg cggcgaggcg tcccatcggc
     8101 caccccacgg acggcgcggc aacgccgtgc ccatcatgct cgcaagctgc cgccaactcg
     8161 cgagtaatcg tttcccctgg aactgcgtca gcaccgccca gtcaaggcgg tgctcggccc
     8221 taccgggctg ccgtcgtttc gcgtgccttg gcgcggggcg ctgcctcgcc ggccggaccg
     8281 gagatcgacg gcttatcggc gctcgcctcc atggaggcat cgtcgtcctc tgccatgttc
     8341 ttcttgaagg acttgatgcc cttcgcgaag tcacccatca gggtgctgac cttgccgctg
     8401 ccgaacagca gcatcacgac gagcaacacg atcagccaat gccaaatgct taagctaccc
     8461 atttgtcctc cgtccggggc aggcgagttg cttgcttcgt tcggcccctg gtcgcgcctc
     8521 gcgcgactac cttaggcgat cttggctctc gctgccagtg gacatggact gcagccgcgg
     8581 gcaatgcaag cgaagtttcc ggccgggcgg agttttgtgc cgattcccgc caatgtgacg
     8641 aatccgcgca ggattcattg cggtccgccg cgctgccgcg tatcgtcatg cacggtgggt
     8701 caacccgcac caaccgcgaa ccggtgccgg gaagctcacc aaagggaagg gcgtccgtcg
     8761 gaaggcaagt ggcaggaccg tcgcacgccc ctgttcatca gggcggctcg tctgcgtttc
     8821 caacagacac cgctcgcagg cagaaaggca acgcgtcgat gcccaagggc accgtgaagt
     8881 ggttcaaccc gaccaaaggc tacgggttca tagcgccgga caccgggggg aaggacgtct
     8941 tcgtccatat cagcgccgtg cagaaggccg gacttcgcag tctgaacgag ggtcagaaag
     9001 tcggtttcga tgtcgagcaa cagcagaacg gccgcactgc ggccgtgaac ctgtcgcaga
     9061 ccgactgacc caggcggctg cacgggccga ggcgacggtc cgttcggcag tttgtttttg
     9121 atcaaatatt gccgcgcggc accagccgcg gggtgaagta cgtccgcacc actggctggc
     9181 tgcgttgaca gccggccagc gcttggccac ggtcggtcct catgcagcac agcttcgatc
     9241 tttccggccg cgtcgccctg gtgaccggca gttcgcgcgg gcttggcctg gccatcgcac
     9301 aggggatggc ggccgccggc gcgcgggtga tcctgcacgg acgcgatgcg gcggcgctgc
     9361 ggacccgtgc cgcggcgctg ccggattgcg cgggcacggc ggcgttcgac gtcaccgatt
     9421 cggcgggcgt cgatgccgcc ttcgcgcgga tcgccgagca acacggccgc ctggacatcc
     9481 tggtgaacaa tgccgggatc ataccgcgca aaccgctgct ggagaccacg gacaaagatt
     9541 ggcaggcggt gatcgactcc aacctgaccg cctatttccg catcgcccgc gagtccgccc
     9601 ggctgatggt gccgcggaac agcgggcgga tcatcatggt gtcgtccatc atgggcctgg
     9661 tcgctcggcc gaccatccct ggctacgtga ccgccaaggc ggggctgcac ggcttggtgc
     9721 gggcgctggc ggtggagctg gcaccgcacg gcatcacggt gaacgcgctg gcgccgggtt
     9781 tctttccaac cgacgcgacg gacgtgctgc acaaggacgc cacgttcaat gcgtggattg
     9841 ccgggcgcgc gccgctggcg cgttggggcg acccgccgga actggccggg ccagcggtat
     9901 tcctcgcctc cgatgcggcg tcctacgtca ccggccacgt gctggtggtg gatggcgggc
     9961 tgagcgcggc gctgtagccg cccttgccgg gcggatcaga ccgcggcgcc gatcagccag
    10021 acgccccagc cgatcagtgc aacgccgctc aatgtgctga tgcgccactt cggcggagcc
    10081 agcttctcca ccagcaggac cagggtcagc acggccatcc acaacaggtt catggccccg
    10141 gcgacgaaca gcaccgccat caatgcccag cagcacacca cgcagtagcc gccatggcgc
    10201 aggccgagcc gcagcgcgcc ggacgtgccg tcgcgccatt cggccaggaa gaacgccagc
    10261 ggggtgcggc atttcgccag gcaacggtcc ttcagcgcgg tgaactggaa catgcctgct
    10321 gcaagcagga tggcggcgct gagccagaca ctggcgctct gcagcatggg cgtcagcagc
    10381 gcggtgcgtg ccagcaccac ctgcaagctg gcggcgagca ctgagtagcc catccatgcc
    10441 gcgccatagc cgccgacgaa cacggccacc gccgccggaa cattgcgcga cgcgtcgcgc
    10501 cgggcggcca gggaggcgaa ggtggcaatg gcccgcaacg cagtcggcac catcatggcc
    10561 accatcatga cgctccacat caccgtcgcg aacagcacct cgagcgcgag cggccggtgc
    10621 ggcatggcca tcggcatgtc gcccgccatc tccggcatgc cggcattcat ctgctgcagc
    10681 cacagccagc agagtccgac gagcacggca agccccgccc acagcggggc ccgcacctgc
    10741 gccgcgcgcg agttcatgcg gctcaggccg acatataggt gaacggggac aggaagccgt
    10801 tgcgtccgga cagcgtccag tccatgccaa aatccgcgaa gcggaactgc gtcgagcgtg
    10861 ccaccgtcgc aggctcgccg ggtgcgaccg ccagcggatg attggagatg gtgatcggtt
    10921 caccgccctg tccggtcatc acctcgattg ccgcggccgc gatgccggga atggtcaggc
    10981 tggcgttctt gcccgtgccc tcgaaatgca tcggcacact cctggcgccg aggatttcgc
    11041 cgatgaacga ggccagtacg gccggatgcc ccccggcctt gccgccaaaa atctggtgca
    11101 gcgcatcgtt ctgcggctgg cttgcccgat cgtcaaagta ggccgccacc ttccatttgg
    11161 tttccaccat ggtgccgggc gcatggatcg ccagcgcgac gttcagcccg tccagcctgg
    11221 tctcgccgaa gctgccctcc cggatatgcc aggccaccag ggcggtgcag tcgccctcgg
    11281 tcggcgggct ggtgaacacg caggggcaag ctgcctcgca attacaggtt tcaaagtagg
    11341 tcccattcag cttccactct gtcattgctg ctctcctggt gatttgactc acagccaacc
    11401 atgccgaacg ctcagtgggc aggagcagcg acaatttcgc ggtgagcaac ggggactgta
    11461 ctggcgagcc gtccagcaca gttcggcggc aggggaggaa gccatgcttg cgttccgcgt
    11521 cagcccgcag gatgcgcggc cactcgtgga gcagatcgtc gcgggcattc gtcagcagat
    11581 ctagacgcgg cagcttcgcc cgggcgtgcg tgtgccctcg atccgcggct ttgccggccg
    11641 gcacggggta agccgctgta cggtggtgga ggcatacgac cgactggtcg cgctcggtta
    11701 tctggaaccg cgccgcggcg cggggttcta caccatggca ccgcgttgcg acggcgcaag
    11761 gtccgcgccg ccacctgaaa cccggcataa cgaggacctg gtctggctga tccgccgcat
    11821 cctggaaggc ggcgaccgta ccgtctgtcc cggcggcccc tggctgccca acgagtggct
    11881 ggacgaggcc agcatccgcc agaacctcaa cgtgcttgct cgccgtaacg gcgcgtatct
    11941 gctggagtat ggcaacccgt ccggctacct cccgctgcgc gagcacttcg ccggcacgct
    12001 cggcgagatc ggcgtcaccg cgcagtcgtc gcagatcgtg ctgacgcaag gcaccagcca
    12061 ggcactggat ttgatcatcc gccggatgtt gcggcccggc gatcatgtgc tggtcgatga
    12121 tccgggctat tacaatctgt tcggcaacct gcgtctcagt gacgtccagc tatgcccggt
    12181 accgcgtctg ccggatgggc cggacatgga cgcattggag cgtctcgcgg cgacgcacca
    12241 gccgaagctg tacttcaccc agtccgtgct gcaaaatcct accggcagta cgatggcgcc
    12301 gcatgtcgcg tttcgcgtgc tgcaggcagc gcagcgctac gacttccgca ttgtcgagga
    12361 tgacatattc tgtgacctcc aggcggcgcc gacgccccga ttggccacgc tcgatcagct
    12421 ccagcgggtg atctatgtcc gcagcttctc gaagacgttg tcggggagtt tgcgcgtcgg
    12481 ttttgctgcg tgccagcagg agatcgccga tgcgctggtc gaagtgaaga tgctgaccag
    12541 catcaccagt tcgcagtttg ccgagcgcct gatctatctg atgctggtcg atgggcatta
    12601 tcgcaagtac atcgcgcggc tgcagaagcg cctggccgac gcgcgggtga acgtgctgcg
    12661 tgcgttcgag caagcggggc tggagctttt catggagccg agcgagggca tgtttctatg
    12721 ggcgcggtcg ccgcgtgtgc cggattcgct gtgcctggcg gaaaaggccg cagcggacgg
    12781 atttgtcctg gcgccgggca ctgtgttccg cccgctgctg gaggagtcgc cgtggatgcg
    12841 gttcaatgtc gcggtttgcg atgccccgaa cgtgcagcgc cggttggaga cgttgttgaa
    12901 cgagtgggtg attccaaccg gctgaagcgg tcaagcgaac accgaccggc tgcgctccct
    12961 gcagttgccc aggcacagaa ccccgcggtg ctgtgctttg cgcatgtgac caaccagatg
    13021 gcccgggttg aaggcgactc tgaaaagggc gaggcggatg gaacggctgg cgcactcgag
    13081 ctgattgccg gcgccgcgcg ggcgtgttcg gatacctcct cctgaagacc ttcgctcgaa
    13141 atcggctgtc actgtcagag ggaaccaaat tgcccatccc cgccctcgca tccccgggcg
    13201 ccatgctggc catgatctcc gccgcactgt tcggcgccag tacgccggtc gcgaagtttc
    13261 tgctggggca gatcaatccc tggctgctgg ccggagtgct ttacctgggc tccggcatcg
    13321 gcctggcggc tgtgcatgtc ggccgccgtt cgtttgctat cgcgcagccc gaggcgccgc
    13381 tgcgccgcgc cgacctgccc tggctcgccg ccgtcgtgct ggccggcggt gtcattggtc
    13441 cgttgctgct gatgctcggc ctcagcgtga cgccggcttc cagcgccgcc ctgctgctga
    13501 acctggaggg cctcgccacg atggcgatcg cctggctggt gttccgcga
//
LOCUS       DASSZH010000066         6325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1109662, whole genome shotgun sequence.
ACCESSION   DASSZH010000066 DASSZH010000000
VERSION     DASSZH010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109662"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VFN42_01330"
     CDS             <1..609
                     /locus_tag="VFN42_01330"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HET8995286.1"
                     /translation="FQSIGTAGGEAMAVIGRADDKRFPNAAALVQYAKAHPNALAVAN
                     GGPGLALKGLDDLDAAAHIQMKSIPYSGGSTILTALLGGHVDLAVTAPSNAVRNPKLR
                     VVMVLTKAKVYAPMPKVETSGGAGYDADAPLQRAVFVKKGTPAPVIAALSKAVDKAVE
                     TPAWKEYAKRFSQIDAASDAAAANAALERSVVDWQQYLKANK"
     gene            606..1136
                     /locus_tag="VFN42_01335"
     CDS             606..1136
                     /locus_tag="VFN42_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995287.1"
                     /translation="MTSTVPDRDLYAAAATAFAADAARSPESSADRLVSIAWASRLFA
                     LLCACVGTYVASFSVIEMRQGHASAWLLPLATGSLVAVLCIAAALTPGPAVLSPRAIG
                     LRSLAIALLVLYAFVLLPAFGFLLASLIFAAAVAFAYAPRRLFVALGGVVMAVALWAL
                     FAYIVAEPLPIGWLWR"
     gene            1133..2602
                     /locus_tag="VFN42_01340"
     CDS             1133..2602
                     /locus_tag="VFN42_01340"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HET8995288.1"
                     /translation="MTAEVLRQAIGLISPLYIVAGTLAGYLVGIIPGLGPAAGITLLL
                     PLTYYMSPAEALIFYASLYQSAEYSGSITAIAVSTPGTPNAAATVLDGYAMNRQGRIG
                     KAFAYSLWSAVFASLVGTAGMLVLGAPMARLALYLGPSEYAALGILGLSCVSILSTDY
                     PLRGLISAILGLMLSTIGLDLLSGVPRFTFGIPSLFDGLPLVALLTGLFALPEAIVLL
                     TEKSAVLQLGTNGGDHRVWLRWREFTGVFPVMCLGTAIGFVMGLIPGLAGSVPPWISY
                     NLAKSVSSERDRFGKGAAAGLVAPEATNAAVMHSTLLPAFSFGIPGTPTSAVILGAMM
                     LSGLTPGPMLFSRHPAIPYAVMISLFLATGFLWLVGLVATNVWTRILTVPQELLAVAI
                     VIFVIVGSYVARNDLFDPQLAVIAGIVGYCLKKAHFSAPALVVGFILGPIIETNIRRA
                     LLLSGGLVSGLPGPVTTALLAGSLLMMAFGVRQSLSGSR"
     gene            complement(2764..3720)
                     /locus_tag="VFN42_01345"
     CDS             complement(2764..3720)
                     /locus_tag="VFN42_01345"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HET8995289.1"
                     /translation="MPDSDFFGGLAKAHPPATRPNTLAALEIVPTGRAVGAEVRGVDL
                     SQPVPAELAAALHSAWLDHLVLLFRDQYLDAAHYLDAARIFGEPVEGGARRYIKAAGV
                     QVDDRFPELTILSNLDESGKPVRDNGALGSYEVVWHSDNSYVEAPPAGSCLYSLEVPT
                     HSGNTSFNNQYRAYETLPDELKQAIAGRRSKQDASRNSAGVLRPGLKKPERPEDVPGP
                     MHPLVRRHPETGRIALYLGRRRIYPSQFIEGLSEAESETLLEKLWAHATRPELSWTHV
                     WRPGDLLVWDNRCAMHYREEVDDTQRRVMFRTQFAGQVPIAA"
     gene            complement(3813..5090)
                     /locus_tag="VFN42_01350"
     CDS             complement(3813..5090)
                     /locus_tag="VFN42_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8995290.1"
                     /translation="MRLQSFLAGRLAGRVHYAWVVVAIMFTVILASVGVRAAPGVLIV
                     PLEHSFGWSDTTISGAISLNILLFGLVGPFATALMQTFGMRRTVLCSMSLLMLGAGSS
                     AFVTTPWQLYLTWGVLVGLGSGAGMVGLAAVVANRWFVQRRGLVVGVLTASNASGQLI
                     FLPILAHLAETLGWYSVPWTVALVIAALIPLVLFLLPESPGSIGIGPYGAAAEPPTLP
                     SAGNPFGVALGGPAKGARSADFWLLVGSFSICGFSTNGLVGTHLIAFCADHGIAEVTA
                     AGLLAMMGVFDLIGTTASGWLTDRFNPRVLLFWYYGLRGLSLAILPFTNFSPLSLSVF
                     AVFYGLDWVATVPPTVALTNEVFGRQDAPVLVAWIFAAHQVGGALAAFGAGAVRNATG
                     SYVAAFLASGVACLLASLLVLRIAGRPVLAIAE"
     gene            5195..5770
                     /locus_tag="VFN42_01355"
     CDS             5195..5770
                     /locus_tag="VFN42_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET8995291.1"
                     /translation="MRKSKEAAAATRQCIIDAAALLFRRHGIDAVGVDAVMQAAGLTH
                     GGFYGHFASKEALVAEVSAAALARSAARWDRISREAPPAEALGRIVGPYLSPAHVAAP
                     ESGCVLTALGAEVARRPEARAALSASIREMLQALARCRPEQPGPAALAGLSTMVGAVV
                     LARLADDPQLADAFLQAAERAVLGRPAGETG"
     gene            complement(5767..>6325)
                     /locus_tag="VFN42_01360"
     CDS             complement(5767..>6325)
                     /locus_tag="VFN42_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8995292.1"
                     /translation="RGTLHAATREGTRMSTKPKHLGHVNLYVRNAERSKQWYQDVLGL
                     HVYDFRPGWAAFMSADTDKSHEVALMEVGENAALPQKGQVGLNHLAWMMESLDDLKVM
                     YHRLKDHGVPIDRIIDHGLSLGIYFRDPDGNGLEVSYELPRAQWPRQDRVFQPDVVGL
                     GRFPGPWEEEMRAQSAAARRAAAPA"
BASE COUNT         1022 a         2077 c         2108 g         1118 t
ORIGIN      
        1 tttcaatcga ttggaaccgc cggaggcgaa gcgatggcgg tgatcgggcg cgctgacgat
       61 aagcgcttcc ccaacgctgc tgcgctggtg cagtacgcca aggcgcatcc gaatgcgctg
      121 gcggttgcca atggcggtcc aggactggcg ctgaaagggc tcgatgacct ggacgccgca
      181 gcacatatcc agatgaagtc gattccctat tctggcggct cgacgattct caccgcgctg
      241 cttggcggcc atgtcgatct cgccgtcacc gcgccgagca acgcggtgcg gaatccgaag
      301 ctgcgcgttg tgatggtgct gaccaaggca aaggtctatg caccgatgcc gaaggtcgaa
      361 acatcgggag gcgccggcta tgatgccgat gcgccgctgc agcgggcggt attcgtcaag
      421 aagggcacgc cggcgccggt tatcgcggcg ctgtcgaagg ccgttgacaa ggcggtggaa
      481 actcccgcgt ggaaggaata cgccaaacgc ttctcgcaga tcgacgctgc atccgacgca
      541 gcggcggcaa atgcggcact ggagcgaagc gtcgtcgatt ggcagcaata cctgaaagcg
      601 aataagtgac ctcgacggtc cccgaccgcg atctttacgc ggccgccgcg accgcctttg
      661 cagcagacgc ggcgcgttcg ccggagagtt cagcagaccg gctggtcagt atagcctggg
      721 ccagccggct gttcgcactg ctctgcgcct gcgtcgggac ctatgtcgcc agtttttcgg
      781 tgatcgagat gcgccagggc catgcctcgg cgtggctgct gccgctcgcg accggatcgc
      841 tggtggcggt attgtgcatc gctgccgccc tcacgccggg ccccgccgtt ctgtcgccgc
      901 gcgccatcgg tctgcgatct ctggcgatcg cactgctggt cctatacgcg ttcgtcctgc
      961 tgccggcgtt cggatttctg ctcgcctcgc tgattttcgc agcggccgtg gcctttgcct
     1021 acgcgccgcg gcgtttgttt gtggcactcg gcggcgtggt gatggcagtc gcgttgtggg
     1081 cgctgtttgc ctatattgtc gcggagccgc tgccgattgg atggctgtgg cgatgaccgc
     1141 cgaggtcctg cgccaggcga tcggattgat ctcccccctc tacatcgtcg cagggacgct
     1201 tgccggttat ctcgtcggga tcattcccgg cctcggcccg gccgctggca tcactctgct
     1261 tctgccgttg acctactata tgtcgccggc cgaggcgctg atcttctatg ccagcctgta
     1321 ccagagcgcc gagtacagcg gttcgatcac cgcgatcgcg gtcagcacgc ccggtacgcc
     1381 gaatgccgcg gcaaccgtgc tcgacggcta tgcaatgaat cgccaggggc ggatcggcaa
     1441 ggcgttcgcc tattcactgt ggtcggcggt gttcgcctcg ctggtcggta ccgccggcat
     1501 gctggtgctt ggcgctccga tggctcggct cgcgctgtat ctcgggccgt cggaatatgc
     1561 cgcgcttggc atacttgggc tgtcctgcgt cagtatcctg tcaaccgatt acccgctgcg
     1621 tggcctgatc tcggccattc tcgggctgat gctgagcacg atcggacttg acctgctgag
     1681 cggcgtgccg cgctttacgt tcggcattcc atcgctgttc gacggactgc cactggtggc
     1741 actgctgacc ggcctgtttg ccttgcccga ggcgatcgtg ctcctgaccg agaagagtgc
     1801 ggtcttacaa ctgggcacta atggcggcga tcaccgggta tggctgcgct ggcgcgagtt
     1861 caccggggtg tttccggtca tgtgtcttgg cacggcgatc ggttttgtga tgggcctgat
     1921 cccgggtctt gccggcagcg tcccaccatg gatttcctat aatctggcga aaagcgtctc
     1981 gtccgagcgc gacaggttcg gcaagggcgc ggcggccgga ctggttgcgc cggaagcgac
     2041 caacgccgcg gtcatgcatt cgacgctgtt gccggccttc agcttcggta tccccggcac
     2101 tccgacttca gcggtcatcc tcggcgcgat gatgctgagc gggctgacgc cggggccgat
     2161 gctgttctcc cgtcatccgg cgattcctta tgccgtgatg atcagcctgt tcctcgcaac
     2221 cgggtttctg tggctggttg gtcttgtcgc gaccaatgtg tggacgcgta tcctcaccgt
     2281 gccgcaggaa ctgctggcgg tggcgatcgt catcttcgtc attgtcggca gctatgtcgc
     2341 gcgcaacgat ctgttcgatc cgcagcttgc ggtgattgcc gggatcgtcg ggtattgcct
     2401 gaagaaggca catttttccg ccccggcgct ggtggtcggg tttatcctgg ggccgatcat
     2461 cgaaaccaac atccgccgcg cgctcttgtt gtccggcggc ctggtcagcg ggctgcctgg
     2521 cccggtcacc actgccctgc tggctgggtc gctgctgatg atggccttcg gcgtgcgcca
     2581 gagcctcagc ggatcccgct agagcatatc cggtgcagca aaacgcaccg gagatgctct
     2641 agccctttgt ctgatcgtgg cattcagggc ttcggcgatt ccgcctgcac cgatcacgct
     2701 tggtacaggg ccggaaatgg gggcccctct gcgatgtatc atgtggcgga cgaagcgccg
     2761 ctgctacgct gcgatcggca cctggccggc aaactgggtg cggaacatga cgcgccgctg
     2821 tgtgtcgtcc acctcttcgc ggtaatgcat cgcgcagcgg ttgtcccaga cgagcaagtc
     2881 ccccggcctc cacacatggg tccagctcaa ttcggggcgg gtcgcgtgcg cccacaactt
     2941 ttccagcagc gtctcgcttt cggcctcgct gagtccctcg atgaactgcg aggggtaaat
     3001 ccggcggcga ccgagataga gcgcaatccg ccccgtctcg ggatgccgac gtaccagcgg
     3061 gtgcatcggg ccgggcacgt cttccggccg ctctggcttc ttgagtcccg ggcgcagcac
     3121 gccagcggag ttgcggctgg catcctgctt cgaacgccgg ccggcgattg cctgcttcag
     3181 ctcatccggc agcgtttcat aggcgcggta ctggttgttg aacgaggtgt tgccgctatg
     3241 ggtcggcact tccagcgaat agaggcagct cccggcgggg ggcgcctcga cataggaatt
     3301 gtccgagtgc cagacgactt catagctgcc gagcgcgcca ttgtcgcgca cgggtttgcc
     3361 gctttcatcg aggttcgaca ggattgtcag ttccggaaac cggtcatcga cctgaacgcc
     3421 ggctgccttg atgtagcgcc gagcgccacc ttccaccggc tcgccgaaaa tgcgcgcagc
     3481 atcgagatag tgcgcggcgt cgagatactg gtcgcggaac agcagcacga gatggtcgag
     3541 ccaggcggaa tgcagtgcgg ccgccaactc ggccggcacc ggctgcgaca aatccacccc
     3601 gcgaacctcc gctccaacgg ctcgcccggt cgggacaatc tccagcgcgg caagtgtgtt
     3661 gggccgggtg gccggcgggt gtgccttcgc aaggccgcca aagaaatccg agtccggcat
     3721 ttctcagtcc tccattccca ccattgccca gcagattata tcacgcctgc aaatgtctca
     3781 aatggcgccg gtgcgccggt cgggagatca cctcactccg cgattgcgag aaccggccgg
     3841 ccggcgatgc gcagcaccag cagtgacgcc agcaggcagg ccacgccgct ggccaggaag
     3901 gccgcgacat agctgccggt ggcgttgcgc accgcgccgg cgccgaacgc ggccagcgcg
     3961 ccgcctacct ggtgcgcggc gaagatccag gccaccagca ccggcgcgtc ctgccggcca
     4021 aacacctcgt tggtcagcgc cacggtgggc ggcacggtcg ccacccagtc cagcccgtag
     4081 aacaccgcga acaccgacag gctgagcggg ctgaaatttg tgaagggcag gatcgccagc
     4141 gacaggccgc gcagcccgta ataccagaac agcagcacgc gcggattaaa ccggtcggtc
     4201 agccagccgg aggcggtggt gccgatcagg tcgaacaccc ccatcatcgc cagcaggccg
     4261 gcggcggtga cttcggcgat gccgtgatcg gcgcagaagg cgatcaggtg ggttcccacc
     4321 agcccgttgg tggaaaagcc gcagatggag aaactgccga ccagcagcca gaaatccgcc
     4381 gagcgcgcgc ctttcgccgg cccccccaag gccacgccga acgggttgcc ggcgctgggc
     4441 agggtcggcg gttcggcggc ggcaccgtat ggcccgatgc cgatgctgcc ggggctttcc
     4501 ggcagcagga acagtaccag cgggatcagc gcggcgatca ccagcgccac ggtccacggc
     4561 acgctgtacc accccagcgt ctccgccagg tgcgccagga tcggcaggaa gatgagctgg
     4621 ccggaggcat tgctggcggt cagcacgccc accaccagcc cgcgccgctg cacgaaccag
     4681 cggttggcca ccaccgccgc cagcccgacc atcccggcgc ccgatcccag cccgaccagc
     4741 acgccccagg tgaggtagag ctgccagggc gtggtgacaa aggcgctgga gccggcgccg
     4801 agcatcagca gcgacatgga gcagagcaca gtgcggcgca tgccgaaggt ctgcatcagc
     4861 gcggtggcaa agggaccgac caggccgaac agcaggatgt tcagcgagat ggcgccggaa
     4921 atcgtggtgt cgctccagcc gaagctgtgt tccagcggca cgatcagcac gccgggcgcg
     4981 gcacgcaccc ccaccgaggc gaggatcacg gtgaacatga tcgccaccac gacccaggcg
     5041 taatgcacgc ggcccgccag ccgtccggcg agaaaggatt gcaggcgcat gccgggcgga
     5101 ctcctggttt gctgttgcgg ctaccatgac ggacgtcata taaacccggc aaggacaaaa
     5161 ttgaccggcg tcatgcgcca cgggaatgct gcggatgcgg aaatcgaagg aagcggcggc
     5221 ggcgacccgg cagtgcatca tcgacgcagc ggccctgctg ttccgccgcc acggcatcga
     5281 cgccgtcggg gtggacgcgg tgatgcaagc cgccgggctg acgcatggcg gcttctacgg
     5341 ccactttgcc tcgaaggagg cgctggtcgc ggaagtctcc gccgcggccc tggcccgcag
     5401 cgccgcacgc tgggaccgga tcagccgtga ggcaccaccg gcggaagcgc tggggcggat
     5461 cgtcggcccc tatctcagtc cggcgcatgt cgccgcgccg gaaagcggct gcgtgctgac
     5521 cgcgctgggg gcggaggtcg cacggcggcc agaggcgcgc gccgccctca gcgcatcgat
     5581 ccgggagatg ctgcaggcgc tggcgcgttg ccggccagag caaccgggac ctgcggcgct
     5641 ggctggcctg tccaccatgg tcggcgcggt ggtgctggcg cggctggccg acgatccgca
     5701 gcttgcggac gcattcctgc aggcagccga gcgggcggtg ctcggccggc ctgccgggga
     5761 gacgggctag gctggcgcgg cggcgcggcg agccgcggcg ctctgcgccc gcatctcctc
     5821 ctcccatggt ccgggaaagc ggccaaggcc gaccacatcg ggctggaaga cgcgatcttg
     5881 ccgcggccat tgcgcccgcg gcaactcgta cgacacttcc agcccgttgc catccggatc
     5941 gcggaagtag atgcccagcg acaggccgtg atcgataatc cggtcgatcg gcacgccatg
     6001 atccttcagg cggtggtaca tcaccttcag gtcgtccagg ctttccatca tccaggccag
     6061 gtggttcagc cctacctggc ccttctgcgg cagcgccgcg ttctcgccga cttccatcag
     6121 cgccacctcg tgcgacttgt cggtgtcggc ggacatgaag gctgcccagc cgggccggaa
     6181 atcatagacg tgcaggccca gcacgtcctg gtaccactgc ttggaacgct ctgcattgcg
     6241 gacgtacagg ttcacgtggc caagatgctt cggcttggtg ctcatgcgcg taccctccct
     6301 ggtcgctgca tgcagcgtac cacgg
//
LOCUS       DASSZH010000067         2609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_615492, whole genome shotgun sequence.
ACCESSION   DASSZH010000067 DASSZH010000000
VERSION     DASSZH010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615492"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VFN42_01365"
     CDS             <1..310
                     /locus_tag="VFN42_01365"
                     /inference="COORDINATES: protein motif:HMM:NF019303.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET8995293.1"
                     /translation="AGGIGSTVLQHTGEVPWPALRLWLDSLLSVRGEDVLRLKGVVRL
                     AGQRRPVVLQGVHHVLHPLASLPATAPVPEDSQIVVIGVRLPPEGLRASFEAALALHA
                     "
     gene            537..1505
                     /gene="lipA"
                     /locus_tag="VFN42_01370"
     CDS             537..1505
                     /gene="lipA"
                     /locus_tag="VFN42_01370"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HET8995294.1"
                     /translation="MSSRLVIDHRVGALRHPEKAHRPDNPVQRKPAWIRVKAPTHPVY
                     QETRKLMRDNRLHTVCEEAACPNIGECWSQRHATMMIMGDTCTRACAFCNVATGQPAA
                     LDAGEPARVADAVARLGLLHVVVTSVDRDDLPDGGAAHFAAVIRAIRASAPGTTIEVL
                     TPDFLRKPGAMEVVAEARPDVFNHNLETVPRLYPSIRPGARYFQSLRLLDAVKRQVPE
                     TFTKSGLMVGLGETRAEIGQVMDDLRIADVDFLTIGQYLQPTVKHAAVDRFVTPEEFA
                     EYAAMARAKGFLLVSATPLTRSSYHADADFAALRAARAARLAPAAA"
     gene            1513..1992
                     /locus_tag="VFN42_01375"
     CDS             1513..1992
                     /locus_tag="VFN42_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014104733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HET8995295.1"
                     /translation="MPTHAERQILPYRPDQLFDLVADVGKYPQFLPWCVGARVRSRTE
                     HELVGDLTIGFGPFRESFTSRVALNRPRQVKVRYENGPFRYLNNQWDFIQHEAGTEVD
                     FYVDFEFRSRILQAAIGVVFNEAVRRMVNAFRKRAGDVYGPPSTLAHTAPTPRTQAG"
     gene            2043..>2609
                     /locus_tag="VFN42_01380"
     CDS             2043..>2609
                     /locus_tag="VFN42_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET8995296.1"
                     /translation="MVYAPVRPDPSLPPLRVNLYSDTQTKPSPAMLEAMARAEVGDEQ
                     HGDDPTVHALCDRMAALLGKQAAVFLPSGTMCNQISLLVHCRPGDEVIAHESAHIIGN
                     EGGGPGALAGALMLGLPGARGQFDVPTLEAALRERRRNVPSQVLVAVEQTANFGGGSV
                     WPLDKLTAVLDAAHANGMQTHMDGARLMN"
BASE COUNT          389 a          920 c          892 g          408 t
ORIGIN      
        1 tgcgggcggt atcggttcca cggtgctgca gcacacgggg gaagttccct ggccggcact
       61 gcggctctgg ctggactcgc tgctgtcggt gcgtggcgag gacgtgttgc ggctgaaggg
      121 tgtggtgcgt ctggccggcc agcgccggcc ggtggtgctg caaggggtgc accacgtgct
      181 gcacccgctg gcgtcattgc cagccaccgc gccggtgccg gaggacagtc agatcgtggt
      241 gatcggcgta cgcctgccgc cggaaggact gcgcgccagc ttcgaagccg cgctggcatt
      301 gcacgcctga cgcatgcagc ccgcgggcgg cggcaccggt ggagcgcctt cggccgcccc
      361 agcgtgtacc gcggtgcctg ctattgccgg tgtattgcta caacccgctg ctatcggaac
      421 ggttcgtccg ggcggcggct gaacaatccg ggaccgcatt ccggcgccgc tgtggcgctg
      481 tgatccaacg agcatgcttg acgccccccg ccgcgcccgc cacttgtagc ggcgccatgt
      541 cctcccgcct cgtcatcgat catcgcgtgg gcgcgctccg ccatccggag aaggcgcacc
      601 gtccggacaa cccggtccag cgcaaacccg cctggatccg ggtgaaggcg ccgacgcatc
      661 cggtttacca ggagacccgc aagctgatgc gggacaaccg gttgcacacc gtgtgcgagg
      721 aggccgcctg cccgaacatc ggcgagtgct ggtcgcaacg ccacgccacc atgatgatca
      781 tgggcgacac ctgcacgcgg gcctgcgcct tctgcaacgt cgccaccggg caacccgcgg
      841 cactggacgc cggcgagccg gcccgcgtcg ccgacgcggt cgcccggctg ggcctgttgc
      901 atgtggtggt cacctcggtg gaccgtgacg acctgccgga tggcggcgcc gcacatttcg
      961 ccgcggtgat ccgcgccatc cgcgcctccg cgcccggaac cacgatcgag gtgctgaccc
     1021 ccgacttcct gcgcaagcca ggcgccatgg aggtggtggc ggaagcccgc cccgacgtgt
     1081 tcaaccacaa cctggaaacg gtgccgcggc tgtatccatc gatccgcccg ggcgcgcgct
     1141 acttccagtc gttgcggctg ctggatgcgg tgaagcggca ggtgccggag acgttcacca
     1201 aatccggcct gatggtcggc ctgggcgaga cccgcgccga gatcggccag gtcatggacg
     1261 acctgcgtat tgccgacgtg gatttcctga caatcggcca gtacctgcaa cccacggtga
     1321 agcacgcggc ggtggaccgc ttcgtcaccc cggaggagtt cgccgaatac gccgccatgg
     1381 cccgcgccaa ggggttcctg ctggtttcgg cgaccccgct cacccgcagc tcctaccacg
     1441 ccgacgcgga tttcgccgcc ttgcgggccg cacgggccgc caggctggcg ccggccgccg
     1501 catgatgcgc tgatgcccac ccacgccgaa cggcagatcc tcccctatcg gcccgaccag
     1561 ctgttcgatc tggtggctga tgtcgggaag tacccgcaat tcctgccctg gtgcgtcggc
     1621 gcccgcgtcc gcagccgcac cgagcatgag ctggtgggcg acctgaccat cggcttcggt
     1681 ccgttccgcg aaagcttcac cagccgggtg gcgctgaacc gcccgcgcca ggtgaaggtg
     1741 cgctacgaga acgggccctt tcgttacctg aacaaccagt gggacttcat ccagcacgag
     1801 gccggcacgg aagtggattt ctacgtcgat ttcgagttcc gcagccgcat cctgcaggcg
     1861 gcaatcggcg tggtgttcaa cgaggcggtg cgccgcatgg tgaacgcgtt ccgcaagcgg
     1921 gcgggcgacg tgtatggtcc gccctccacc ctggcgcaca ccgcgccgac cccgcgcacc
     1981 caggcaggct gacagcagcg cccggtcctg ctttgttggg gcgccacctg cggagcccca
     2041 ccatggttta tgcccccgtt cgcccggatc cctcgctgcc gcctttgcgg gtgaatctgt
     2101 attccgacac ccagaccaag ccctctccgg ccatgctgga ggcgatggcg cgcgccgaag
     2161 tgggagacga gcagcacggc gacgatccga ccgtgcacgc gctgtgcgac cgcatggcgg
     2221 cgctgctggg caagcaggcg gcggtgttcc tgccttccgg aacgatgtgc aaccagattt
     2281 ccctgctggt gcattgccgc ccgggcgatg aggtgatcgc gcacgagagc gcccacatca
     2341 tcggcaacga gggcggcggc cccggcgccc tggccggcgc actgatgctg ggcctgccgg
     2401 gtgcgcgcgg gcagttcgac gtgccgacgc tggaggcggc gctgcgcgaa cgccggcgca
     2461 acgtgccgtc ccaggtgctg gtcgcggtgg aacagaccgc caatttcggt ggcggcagcg
     2521 tctggccgct ggataagctg accgccgtgc tggacgccgc gcacgcaaac ggcatgcaga
     2581 ctcacatgga cggcgcccgc ctgatgaac
//
LOCUS       DASSZH010000068         2066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_479012, whole genome shotgun sequence.
ACCESSION   DASSZH010000068 DASSZH010000000
VERSION     DASSZH010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479012"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="VFN42_01385"
     CDS             <1..1018
                     /locus_tag="VFN42_01385"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8995297.1"
                     /translation="PENGRQANAVPRVAAVKPSFTVEDVYQRASRTLEKCLRHGATRM
                     RTHLELDAGVELRSFEAIEQLRRDYAWAIDIEVCVFPQEGLTNNPSADALLVQALQRG
                     AKVIGAAPNYDPDHAGQIHRVFALAREFGVDIDMHLDSGSTAEAMDIHLVCELTSLYG
                     MGGRVAVGHGSKYAALPPADLATLARRMADTGVALTVLPATDLFMTGRDRDHSVVRGV
                     ADVNFFAAHGVNCSLSTNNVLNPFTPFGDGSLLRMANLQANVCQIGQAQQLRDCFAML
                     TERSARLMNLNDYGIRVGNPADIVVLDAASPEQAVAEITPPLAVFKRGHRTVTRTRTE
                     LHPP"
     gene            1065..1367
                     /locus_tag="VFN42_01390"
     CDS             1065..1367
                     /locus_tag="VFN42_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HET8995298.1"
                     /translation="MRLAEIAQVIRSKNAGPRRLTLDLMFASDADYQRVAQSPALARA
                     EIAALYRVAPEDVTVIPYPVGRAIKIVLARKIMAGDPGDFDVYGAQQHAPLLAVEI"
     gene            1371..>2066
                     /locus_tag="VFN42_01395"
     CDS             1371..>2066
                     /locus_tag="VFN42_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995299.1"
                     /translation="MSASGRILDLLRRARRPMRLVGASGQLGYGVPTPAFEAALARQP
                     DLIGADMGSIDIGPTYLGKGEMATAPAATRRDLRKLLHGARTLDVPLVIGSAGSAGAA
                     PHLDATLAIVREIARADGLHFRLGVIRADLPRPMLLEAVRAGRVRGIDDMPALTEEDV
                     TEAAQLVGQMGMGPFRRALAEDIDVLIAGRACDTAIFASLPTMLGYPTGLSVHMAKII
                     ECASLCCIPGGRDC"
BASE COUNT          331 a          770 c          658 g          307 t
ORIGIN      
        1 cccggaaaac ggccgccagg ccaacgcggt gccgcgcgtg gcggcggtca aacccagctt
       61 caccgtggag gacgtttacc agcgcgccag ccgcacgctg gagaagtgcc tgagacacgg
      121 cgccacccgg atgcgcacgc atctggaact cgatgccggg gtggaattgc gcagcttcga
      181 ggcgatcgag cagctccgcc gcgactacgc ctgggcaatc gacattgagg tctgcgtctt
      241 cccgcaggag ggcctgacca acaatccaag cgccgatgcg ctgctggtac aggcgctgca
      301 acgcggtgcc aaggtgatcg gcgccgcgcc gaactatgat ccagaccatg ccggacagat
      361 ccaccgcgtc ttcgccttgg cgcgggaatt cggcgtcgat atcgacatgc acctcgattc
      421 cggcagcaca gcggaggcca tggacatcca tctggtctgc gaattgacct cgctgtacgg
      481 catgggcgga cgggtggcgg tcggccacgg gtcgaaatac gccgccctgc ccccggccga
      541 tctggcaact ctggcacgtc gcatggccga cacgggcgtc gcgctcacgg tgctgcccgc
      601 caccgacctg ttcatgaccg gccgcgaccg cgaccacagc gtggtccgcg gcgtcgccga
      661 cgtgaacttc ttcgccgcgc acggggtgaa ctgctccctc tccaccaaca acgtgctgaa
      721 ccccttcacc ccgttcggcg acggctccct gctgcgtatg gccaacctgc aggcgaatgt
      781 ctgccaaatc ggccaggcgc agcagctacg ggactgcttc gccatgctca ccgagcgctc
      841 cgcccggctg atgaacctga acgactacgg catccgggtc ggcaaccctg ccgacatcgt
      901 ggtgctggat gccgcctcgc cggaacaggc cgtggcggaa atcactccgc cactggcggt
      961 cttcaaacgc gggcaccgaa cggtgacccg cacgcgaacc gaactgcatc ccccatgagc
     1021 tgccccaccc cgccaagcgc caccccctgc tgaaagactg accgatgcgc ctcgccgaga
     1081 ttgcccaggt catccgcagc aagaacgccg gaccgcgccg gctgacgctg gacctcatgt
     1141 tcgcatccga cgccgactac cagcgggtcg cgcagtcccc tgcactggcc cgggcagaga
     1201 tcgcggcctt gtaccgcgtg gcgccggagg acgtgacggt gatcccctat cctgtcggca
     1261 gagccatcaa gatcgtcctg gcacgcaaga tcatggcggg ggacccgggc gatttcgacg
     1321 tctacggcgc ccagcagcac gccccgctgc tcgccgtgga aatctgaccc gtgagcgcct
     1381 ccggccgcat cctggacctg ctccgccgcg cccgccgccc gatgcgcctg gtcggcgcct
     1441 ccggccagct cggctacggc gtgccgaccc ccgccttcga ggcagcgctc gcccgccagc
     1501 ccgacctgat cggcgccgac atgggctcca ttgatatcgg cccgacctac ctgggcaagg
     1561 gcgaaatggc caccgccccg gccgccacgc ggcgcgacct gcgcaagctg ctgcacggcg
     1621 cccgcacgct ggacgtgccc ctggtgatcg gctccgctgg atcggccggc gccgcccctc
     1681 acctggacgc gaccctcgcc atcgtgcggg agatcgcccg ggccgacggg ttgcatttcc
     1741 gcctcggcgt gatccgcgcc gacctgccgc gcccgatgct gctggaggcg gtgcgcgccg
     1801 gaagggtccg cggcatcgac gatatgcctg cattgaccga ggaggacgtc accgaggccg
     1861 cgcaactcgt cggccagatg ggcatgggcc cgttccggcg cgccctggcc gaggacatcg
     1921 acgtgctgat cgccggacgc gcctgcgaca ccgcgatttt cgcgtccctg cccaccatgc
     1981 tgggctatcc caccggcctt tccgtgcaca tggcgaagat catcgagtgc gcgtcgctgt
     2041 gctgtatccc cggtgggcgg gactgc
//
LOCUS       DASSZH010000069         2216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1041754, whole genome shotgun sequence.
ACCESSION   DASSZH010000069 DASSZH010000000
VERSION     DASSZH010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041754"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /gene="sppA"
                     /locus_tag="VFN42_01400"
     CDS             <1..798
                     /gene="sppA"
                     /locus_tag="VFN42_01400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HET8995300.1"
                     /translation="GFTRSHVARLSVNGIITEDRSVTRAVDKLADDPSVKALIVSINS
                     PGGSVAGGESLHDAIARVAAKKPVVAVMRGEAASAAYMIAVAADRIYARQATLTGSIG
                     VLLEAPQFGGLLDKLGVGVTTIVSGPLKDQPSYTHPLSDQGKTVLQGLVMDMYGQFVA
                     MVAQGRHMDPAKVRALGDGRAYTGRQALQLGLVDAIGGEQAARSWLASAKGVRASLPV
                     DDVTTSSFAARELSGMLGPLFEGFWKSVFSQSVMLDGGWSVWHRFDG"
     gene            838..1158
                     /locus_tag="VFN42_01405"
     CDS             838..1158
                     /locus_tag="VFN42_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HET8995301.1"
                     /translation="MTKSELIAELASANPHLRGADVELIVATIFDEITRALARGERVE
                     LRGFGAFTVKHRDARTGRNPRTGEPVPVDEKAVPFFKAGKELRERVNRGKPVQTTGSE
                     RVPA"
     gene            complement(1233..>2216)
                     /locus_tag="VFN42_01410"
     CDS             complement(1233..>2216)
                     /locus_tag="VFN42_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HET8995302.1"
                     /translation="GMACAQQLARAGHDVTLFEKSDRIGGLLRYGIPDFKMEKHHIDR
                     RIAQMAAEGVTFRPGVEVGVTVDMDFLMGEYDAIALTGGAEWPRDLEVPGRELPGIHF
                     AMDYLTQQNKRGAGDDEATAAPDGGISADGKHVIVIGGGDTGADCIGTAARQGAASIL
                     QLEILPKPPEKENKALTWPDWPLKLRTSPAHEEGAERDWAVLTRRAVGVDRVQALECV
                     RVEWAKGDDGRFSMREIPGSTFELKADLVLLAMGFLGPRRGLLEQAGLALDARGNVLA
                     NTVDYQTSVAKVFAAGDMRRGQSLVVWAIREGRQCARAVDQFLMGTSLLPA"
BASE COUNT          332 a          792 c          749 g          343 t
ORIGIN      
        1 ggcttcacgc gcagccatgt cgcccggctg tcggttaacg gcatcatcac cgaagaccgc
       61 agcgttacgc gcgcggtgga caagctcgcc gacgatccgt cggtcaaggc gctgatcgtg
      121 tcgatcaaca gccccggcgg cagcgtcgcc ggtggcgaga gcctgcacga cgccatcgcc
      181 cgggtcgccg cgaagaaacc ggttgtcgcc gtgatgcgcg gcgaggcggc ctccgccgcc
      241 tacatgatcg cggtcgccgc cgaccgcatc tacgcgcggc aggcgacgtt gaccggctcg
      301 atcggcgtgc tgctggaagc cccgcaattt ggcgggctgc tcgacaagct cggcgtcggc
      361 gtcaccacca tcgtatccgg accgctgaag gaccagccga gctacacgca cccgctgagc
      421 gatcagggaa agacggtgct gcaggggctg gtgatggaca tgtacggcca gttcgtcgcc
      481 atggttgccc aggggcggca catggacccg gcgaaggtgc gcgcgcttgg cgacggccgc
      541 gcctataccg gccgccaggc gctgcaactc gggctggtgg acgcgatcgg cggcgagcag
      601 gcggcccggt cctggctggc ctcggccaag ggcgtgcggg ccagcctgcc ggtggacgac
      661 gtcaccacca gcagcttcgc cgcacgggag ctttccggca tgttgggtcc gctcttcgag
      721 gggttttgga aaagcgtgtt ttcccaaagc gttatgcttg acggtggatg gtcggtctgg
      781 catcgtttcg acggctgaac cgttgcagcc gccaggaacg gggcagagga ccgcaaggtg
      841 accaagtcgg agctgattgc tgaactggcg agcgccaatc cgcatttgcg cggcgctgac
      901 gtcgaactga tcgtggcgac gatcttcgac gagatcaccc gcgccctggc gcgcggcgag
      961 cgggtggaac tgcgcggctt cggtgccttc acggtgaagc accgcgacgc ccgcaccggc
     1021 cgcaacccgc gcaccggtga gccggttccg gtggacgaaa aggcggtgcc cttcttcaag
     1081 gccggcaagg agctgcggga acgagtcaac cgcggtaagc ccgtgcagac caccggcagc
     1141 gagcgcgttc ccgcctgacc ggcgggcgga ccgcagcgcg gccccaccgc gttacgcggc
     1201 gcccgctact tacgccgtgc gcgccgcgcc actcacgccg gcagcaggct ggtccccatc
     1261 aggaactggt ccaccgcgcg ggcgcattgc cgtccctcgc ggatcgccca caccaccagc
     1321 gactggccgc ggcgcatgtc gccggcggcg aacaccttcg ctaccgaggt ctggtagtcc
     1381 accgtgttcg ccagcacgtt gccgcgtgcg tccaacgcca gcccggcctg ctccagcaat
     1441 ccgcgccgcg gcccgaggaa gcccatcgcc aggagcacga ggtcggcctt cagctcgaag
     1501 gtgctgccgg ggatctcgcg catgctgaac cggccgtcat cgcccttggc ccactccacc
     1561 cgcacgcatt ccagcgcctg cacccggtcc acgccgaccg ctcgccgggt cagcaccgcc
     1621 cagtcgcgtt ccgcgccttc ttcatgcgcc ggcgaggtgc gcagcttcag cggccagtcc
     1681 ggccaggtca gcgccttgtt ctccttctcc ggcggcttcg gcaggatctc cagctgcagg
     1741 atggaggccg ccccctggcg cgccgcggtg ccgatgcagt cggccccggt gtcgccgcca
     1801 ccaatcacga tcacatgctt gccgtccgcg ctgatgccgc cgtccggcgc cgcggttgcc
     1861 tcgtcgtcac cggcgccgcg cttgttctgc tgcgtcaggt agtccatggc gaaatggatg
     1921 ccgggcagct cgcggccggg cacttccagg tcgcgcggcc attcggcgcc gccggtcagc
     1981 gcgatggcat cgtactcgcc catcaggaaa tccatatcga ccgtcacgcc gacttcgacg
     2041 ccggggcgga aggtgacgcc ctccgctgcc atctgcgcaa tgcggcgatc gatgtggtgc
     2101 ttctccatct tgaagtcggg aatgccgtag cgcagcaggc cgccgatgcg gtccgacttc
     2161 tcgaacagcg tcacgtcatg cccggcgcgg gccagttgct gcgcgcaggc catgcc
//
LOCUS       DASSZH010000070         3308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_513300, whole genome shotgun sequence.
ACCESSION   DASSZH010000070 DASSZH010000000
VERSION     DASSZH010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513300"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1271
                     /locus_tag="VFN42_01415"
     CDS             <1..1271
                     /locus_tag="VFN42_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995303.1"
                     /translation="ALLLAPLCIGSARAESGGMQAARAQDLLNGFGINTHLDGCCNGN
                     GGNYNDTDAVIRAIRYIGKVKLLRDFCESDKLLRIAPRVTAATGAHFWCAIGETRPKG
                     YPEQLELMRRGHRDHWVVAYEGPNEPDAPYVGSLGGPFLRQAAAFMPTLHRAAQQDGV
                     AAIQTSFGVVWPHSDYGTTGDLAADADYGNAHVYPQACPNCLGAGNKGAIAWMDREAR
                     KTTPGKPVAISEWGYTTPAHAGYGAVSEAAQAAYALEFILDAHQAGDPYYIYYGLLND
                     GSGNFGLFHNDFMPKKSAVALHDLFALLHDPSPRATSFTPGRLDVHLHGMPRAEANAG
                     GRSALFQQADGIFWLALWNEQILNDYRGDNHDVHVAPVPVRVSFDRPVESITEYDPVD
                     HGTAPLASGKGSSFALQLPAHPVLLRIDRK"
     gene            1317..2558
                     /locus_tag="VFN42_01420"
     CDS             1317..2558
                     /locus_tag="VFN42_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003628268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET8995304.1"
                     /translation="MPDPTREAAFDLLSAVLDRRRPLEDALDALPEMPARDRAAAHRL
                     AATVLRRMGTLDAVLEPLLRKQPPEPVRHVLRLGAAGLLLLDTPRHAAVGTAVALARS
                     RGLTPFAGLVNAVLRRVADLGSAALEERDAPRLDTPAWLWASWGAQARAIALAHQHEA
                     PLDLTLRPGAEAPQGGEALPTGTLRFPPGTRVAEIPGYATGAWWVQDAAAALPARLLA
                     VRPDETVADLCAAPGGKTAQLAAAGATVTAVERDPARLRRLQENLRQWHLTATLVQAD
                     AAAWTPPAPLDAVLLDAPCSATGTIRRHPDVLRIKRPQDVRALTEAQDRLLAAALGML
                     RPGGRLIYSVCSLQPEEGPARLAAVLARGGVRHDPFLPAELPNLPEAIAPDGTLHTHP
                     GLWPERGGMDGFFAARLIRAE"
BASE COUNT          523 a         1142 c         1158 g          485 t
ORIGIN      
        1 gcgcactgct gctcgcgccg ctctgcatcg gctcggcaag ggcggaatcc ggcggcatgc
       61 aggccgcccg tgcgcaggac ctgctgaacg gcttcggcat caacacgcat ctcgacggct
      121 gctgcaacgg caatggcggc aactacaacg acaccgacgc ggtgatccgg gccatccgct
      181 acatcggcaa ggtcaagctg ctgcgcgact tctgcgaaag cgacaagctg ctgcgcatcg
      241 caccacgcgt caccgccgcc accggcgcgc atttctggtg cgccatcggc gaaacccgcc
      301 cgaaaggcta tccggagcaa ctggaactca tgcggcgcgg ccatcgcgac cactgggtgg
      361 tggcctatga agggccgaac gaaccggacg cgccgtatgt cggcagtctt ggcgggccgt
      421 tcctgcgcca ggcggcggcc ttcatgccga cgctgcaccg cgcggcacag caggacgggg
      481 tagccgcgat ccagaccagc ttcggcgtgg tctggccgca cagcgattac ggcaccaccg
      541 gcgatctcgc cgccgatgcc gattacggca atgcgcatgt gtatccgcag gcctgtccga
      601 actgcctggg ggccggcaac aagggcgcca ttgcctggat ggatcgcgaa gcgcgcaaga
      661 ccacgccggg taagccagtg gcaatcagcg aatggggcta caccacgccg gcccacgcgg
      721 gctatggcgc ggtgagtgag gcggcacagg cggcctatgc gctggaattc atcctggacg
      781 cgcaccaggc cggcgatccg tattacatct attacggcct gctgaatgac ggctcgggca
      841 atttcggcct gttccacaac gacttcatgc cgaaaaaatc cgccgtggcg ctgcatgatc
      901 tgttcgccct gctgcacgat ccgtcgccca gggcgaccag cttcacgcca ggccggctgg
      961 atgtgcatct gcatggcatg ccgcgggccg aggcgaatgc cggcggccgc tccgcgctgt
     1021 tccagcaggc ggacggtatc ttctggctgg cactgtggaa cgagcagatc ctcaacgact
     1081 atcgcggcga caaccatgat gtgcacgtcg cgccggtgcc ggtgcgcgtc agcttcgacc
     1141 ggccggtgga gagcatcacc gagtacgatc cggtggatca cggcaccgcg ccgctggcat
     1201 ctggcaaggg cagcagtttc gctctgcagc ttccggcgca tccggtgctg ctgcggatcg
     1261 accgcaaata gcgccgccgc tggcggccgg gcgcgatccg gcgcatgaga agcccgatgc
     1321 ccgacccgac ccgagaagcc gccttcgatc tgctgtccgc cgtgctggac cgccgccgtc
     1381 cgctggagga tgcgctggac gcgttgcccg agatgccggc gcgcgaccgc gccgccgcgc
     1441 accggctcgc ggccacggtg ctgcgccgga tgggcacgct cgacgcggtg ctggagcctc
     1501 tcttgcggaa gcagccgccg gagccggtgc ggcacgtgct gcgcctcggc gccgccgggc
     1561 tgctgctgct ggacacgccg cgtcacgcgg cggtgggcac cgcggtggcg ctggcccggt
     1621 cgcgtggcct gacccccttc gccggactgg tgaacgcggt gctccgccgg gtcgccgacc
     1681 tgggctcggc cgcgctggag gaacgcgatg cgccgcggct ggatacgcca gcctggctct
     1741 gggcatcctg gggcgcgcag gcccgcgcca tcgcattggc gcaccagcat gaggcgccgc
     1801 tcgacctgac gctgcgtccc ggtgccgagg cgccgcaagg gggagaggcg ttgccgaccg
     1861 gcacgctgcg cttcccgccc ggcacaaggg tggccgagat ccctggctac gccaccggcg
     1921 cgtggtgggt gcaggacgcc gctgccgcac tgccggctcg cctgctggcg gtgcggccgg
     1981 acgagacggt ggctgatctg tgcgccgcgc ccggtggcaa gaccgcgcaa ctggcggcgg
     2041 ccggcgcaac cgtgaccgcg gtggaacgcg atcccgcccg cctgcgccgc ttgcaggaga
     2101 acctgcggca gtggcacctg acggcgaccc tggtgcaggc cgacgccgcg gcctggacgc
     2161 cgccggcgcc actggatgcg gtgctgctgg atgcgccgtg cagcgccacc ggcacgatcc
     2221 gccgccatcc cgacgtgctg cgcatcaagc gcccgcagga tgtgcgcgcg ctgacagagg
     2281 cgcaggaccg cctgctcgcc gcagcgttgg gcatgctgcg ccccggcggc cggctgatct
     2341 acagcgtgtg ttcgctgcag ccggaggaag gaccggcgcg gctcgcggcg gttctggcac
     2401 gcggcggcgt gcggcacgat ccgttcctgc ccgcggaact gccgaacctg ccggaagcga
     2461 tcgccccgga cggtactctg cacacgcatc cgggcctttg gccggagcgc ggcggcatgg
     2521 acggcttctt cgcggcgcga ctgattcgcg cggagtaggg gaggcgacgc gccgtcgcgg
     2581 cgcgcgtcaa agggattttc agccgcagat gaacgctgat gcacgccgat gctgcggtgg
     2641 gacccgatcg gccggaccaa tccccggcaa aggcaaagat tcgtgcttgc gatacttcgg
     2701 cctgctgcca acgggtgcaa gttccgactc ctccgtgtca tgcacgggct tggcccgtgc
     2761 acccacgtct tgctgtggcg caggcagcaa gacatgggtg gccgggccaa gccggccatg
     2821 acacgacgga ggagacgctc cgccggaacg agtcagaacg attggtcact cgtaccagtg
     2881 aaagtcctgc ggctgcattg cgggccgctc atcgtagtca gcctcggtga tgacaccccg
     2941 acagcagtgc cgcgtcgtgg cgtgcgcagg ccgcaaagtg ctaacataag cccgtaagcg
     3001 cggcagccgc gaacccttga gccgcggccg ggcttgaccc ggccatccac gcgagaccag
     3061 gcgtattccg ttgcacccgg cccgatcgtt gccgggtaga tggccgggtc aagccggcca
     3121 caccgtagag gaacgcgttc gcaccggctt gcgttcgcga cgatgagcgc aatcccgcca
     3181 tccacgattt cgttgcgtac aactgaggaa agtcgtggat gccggcatgc gccggcatga
     3241 cggggtggcg gccgaagagt cgcgctgatc gcaagctgtc agcatcagca tcagcatcag
     3301 catcagca
//
LOCUS       DASSZH010000071         2092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1195155, whole genome shotgun sequence.
ACCESSION   DASSZH010000071 DASSZH010000000
VERSION     DASSZH010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195155"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1338)
                     /locus_tag="VFN42_01425"
     CDS             complement(<1..1338)
                     /locus_tag="VFN42_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HET8995305.1"
                     /translation="MADGGNIDTRTARISAEEALALHASGRPGKLETRITKALTTARD
                     LSLAYSPGVAEPCLHIARDPNLAYDYTTKGNMIAVVSNGTAVLGLGNLGALGAKPVME
                     GKVALFKRFADIDGIDLEIASEDVDEIVNAVRFLAGFGGINLEDIKAPECFVIEQRLR
                     ELMDIPVFHDDQHGTAIVAAAGLINALHLTNRDLRETKLVVNGAGAAGIACVELLKSM
                     GMRADNVILCDTKGVIYQGRTEGMNQWKSAHAAPTKARTLAEALEGADVFFGLSAKGA
                     VTPDMVAAMAGKPVIFAMANPDPEITPEEAREATPNAIIATGRSDYPNQVNNVLGFPY
                     IFRGALDVRASTINEAMKIAAAEALAKLAREDVPDEVHITSGGHRPTFGPEYIIPNAF
                     DPRLISWVPPAVARAAMDTGVARKPLADLRRYARELSGRLDPTAGALDAIMESA"
     gene            complement(1652..>2092)
                     /locus_tag="VFN42_01430"
     CDS             complement(1652..>2092)
                     /locus_tag="VFN42_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019088299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET8995306.1"
                     /translation="VAEADVILHVRDAAHPESAAQKADVTAVLEGMVADGTLDPAWPS
                     RTIEVLNKSDLLGGVGAVSTRPGAVAVSAITGEGLTDLAAAIDARIAAGMETADYAIP
                     PQDGARLAWLYQHGEVVERQDDEAGSHVRVRLLPADRARFERQE"
BASE COUNT          292 a          696 c          752 g          352 t
ORIGIN      
        1 ggccgattcc atgatggcgt cgagcgcgcc ggcggtgggg tccagccggc cggacagttc
       61 ccgcgcgtag cggcgcaggt cggcgagcgg cttgcgcgcc acgccggtat ccattgcggc
      121 gcgggcgacg gccgggggca cccaggagat caggcgggga tcgaaggcgt tcgggatgat
      181 gtattccggg ccgaaggtcg gccggtggcc gccggaggtg atgtgcacct cgtcggggac
      241 atcttcgcgt gccagcttcg ccagggcttc cgccgcggcg attttcatcg cctcgttgat
      301 ggtggaggcg cggacgtcca gcgcgccgcg gaagatgtag gggaagccca gtacgttgtt
      361 cacctggttc ggatagtcgc tgcgcccggt ggcgatgatc gcgttcggtg tggcctcgcg
      421 cgcttcctcg ggggtgattt ccggatcggg attggccatg gcgaagatga ccggcttgcc
      481 ggccatggcg gccaccatgt ccggcgtgac cgcgcccttg gccgacaggc cgaagaacac
      541 gtcggcgcct tccagcgcct ccgccagcgt acgcgctttg gtcggagcgg cgtgggccga
      601 cttccactgg ttcatgccct cggtgcggcc ctggtagatc acccccttgg tgtcgcacag
      661 gatgacgttg tcggcgcgca tgcccatcga cttgagcagc tcgacgcagg cgatgcccgc
      721 cgcgccggcg ccgttcacca ccagtttggt ctcgcgcaga tcgcggttgg tcagatgcag
      781 cgcgttgatc agcccggcgg cggcgacgat cgccgtgccg tgctgatcgt cgtggaacac
      841 cgggatgtcc atcagctcgc gcagacgctg ctcgatgacg aagcattccg gagccttgat
      901 gtcctccagg ttgatgccgc cgaagccggc caggaaacgc acggcattga cgatttcgtc
      961 cacatcctca gaggcgattt ccaggtcgat gccgtcgatg tcggcgaagc gcttgaacag
     1021 cgcgaccttg ccctccatca ccggcttggc gccgagcgcg cccaggttgc ccagccccag
     1081 gaccgcggtg ccgttcgaga cgacggcgat catgttgccc ttggtggtat agtcgtaggc
     1141 gaggtttgga tcgcgcgcga tatgcaggca gggttcggca acgccggggg aataggccag
     1201 cgacaaatcg cgtgcggtgg tcagcgcctt ggtgatgcgg gtctccagct tgccggggcg
     1261 gccggaggcg tgcagcgcca gagcctcctc tgcggaaatg cgcgcggtgc gggtgtcgat
     1321 gttgccgccg tcggccatgg ctgtttcccc cgaaaaggat ggtagcggca tcatgggcgc
     1381 gcccgcggcg gttgcaaagc gggcgggtgc acgccccgcc ggagcgctca ggcgcccgca
     1441 cgcggcggaa cgacggcgcg gcggcgttcc aggctttccg agggccagaa gcttgctggg
     1501 cggtcgcggc accgggttgc ttgtccccag ctccggcgtc acgccccggg acacgacttt
     1561 accggatgca ggcgtggcct gtgcgcagca gatgggcagc cgcggcagtg atctccgata
     1621 aatccaggcc cgacgcctgg gctgcgacga cctactcctg gcgctcgaac cgcgcgcggt
     1681 ccgccggcag caggcgcacg cggacgtggc tgccggcctc gtcgtcctgc cgctccacca
     1741 cctcgccatg ctggtacagc caggccagtc gagccccgtc ctgcggcggg atggcatagt
     1801 cggccgtctc catcccggcg gcgatccggg cgtcgattgc agcggcgaga tcggtcaggc
     1861 cctcgccggt gatcgccgaa accgccacgg caccgggtcg ggtcgagacc gcgccgacgc
     1921 cgcccagcag gtcggatttg ttcagcactt cgatcgtgcg cgacggccag gccgggtcca
     1981 gcgtgccgtc ggccaccatg ccttccagca ccgcggtgac gtccgctttc tgcgccgcac
     2041 tttccggatg cgcggcgtcg cgcacgtgca ggatgacgtc tgcctcggcc ac
//
LOCUS       DASSZH010000072         3502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1467855, whole genome shotgun sequence.
ACCESSION   DASSZH010000072 DASSZH010000000
VERSION     DASSZH010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467855"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VFN42_01435"
     CDS             <1..248
                     /locus_tag="VFN42_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HET8995307.1"
                     /translation="AFRMRMPVSVCGEMAASPRLVPLLLGLGLRSFSMNAAAVPRVKQ
                     AIRSVEIDACARFARRVMEQSDPARIDELVSEFGKSG"
     gene            complement(326..1357)
                     /gene="pdxA"
                     /locus_tag="VFN42_01440"
     CDS             complement(326..1357)
                     /gene="pdxA"
                     /locus_tag="VFN42_01440"
                     /inference="COORDINATES: protein motif:HMM:NF002992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HET8995308.1"
                     /translation="MTQRPLLAVTMGDPAGIGPEIAVRALLDPEVRAVSRSFLIGDAR
                     VFQRALSVCGLSAALNRIPGPEALADRAGTIDVLHQDSADPDMLQMGKVQPLGGQAAY
                     AAIRSAIDLAMAGRVDGVATTPINKESLKAAKIPFIGHTEMFAEHTGAREEMTMFTIS
                     GLKIFFLTRHLSLLDACRSITAERVEKGIDNCIKALRQLGLEQPHLAVAALNPHGGED
                     GLFGREEIDGIKPAIAAARARGLHVSGPVPADSVFHMAKIGRYDAVLSLYHDQGHIAA
                     KMLDFEKTVSVTLGLPILRTSVDHGTAFDIAGTGKASAVSMIEAIKVAAEYAVRGVRL
                     GETTPAAAA"
     gene            1509..2063
                     /locus_tag="VFN42_01445"
     CDS             1509..2063
                     /locus_tag="VFN42_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HET8995309.1"
                     /translation="MRLLPLCCAIFALLAGCAPGHGLRPLPRIAVGDYRLGPGDSVRL
                     TTFGEEQLTGEFRVGANGMVAVPLLGEVRAAGLTPTQLQDAVAAGLKRRNLLRDPNVA
                     AEVVEYRPIFVLGEVNKPGQYPYQPGMTMVTAVAVAGGFTYRAVQGYASVVRTVDGAA
                     EERKASRDAFVQPGDVLTVFERRF"
     gene            complement(2137..>3502)
                     /locus_tag="VFN42_01450"
     CDS             complement(2137..>3502)
                     /locus_tag="VFN42_01450"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8995310.1"
                     /translation="LAIDLPLTDGVRREEALARDLRLGPQTKALRHLAHAERVAARAP
                     VQAAVWPVRQVGVVGGGTMGSGIAVALADAGLDVTLVERDSEAEAAARVRVRAIYDRQ
                     LSSGRLAERLYRERTGRIAYATELRALRHADLVIEAVVEDLDEKRRVFRALSGMVRRD
                     TLLASNTSYLDIDLLADEVDAPERVLGLHFFSPAHVMRLVEVVRTRRLWPEALATALA
                     TVQLLGKQPVVTGVCDGFIGNRILGKWRAQAEYALEDGATPAQVDAALEAYGMAMGIF
                     AVFDLAGLDVDWRDRQRKAGTRDPRERYVPVFDWICEQGHYGQKTGRGFYIHEAGKRI
                     ADPEVVAMAERASALRGIARRAISAEEVQQRVHAAMVNEAARVLADGIAARPSDIDVV
                     LVHGYGFPAWRGGPLHEADAVGIAEMLRRVEALYSASGAGWEPAPLLREMAAAGKRFA
                     DLNG"
BASE COUNT          526 a         1261 c         1172 g          543 t
ORIGIN      
        1 ccgcgtttcg catgcgcatg ccggtttccg tatgcggcga aatggccgcc agtccacgcc
       61 tggtgccgct gctgctgggg ctcggcctgc gcagcttcag catgaacgcc gcggcggtgc
      121 cacgggtgaa gcaggcgatc cgctcggtag agatcgacgc ctgcgcgcgg ttcgcgcgac
      181 gggtgatgga gcaatccgat ccggcgcgga tcgatgaact ggtcagcgag ttcggcaaga
      241 gcggctgacc gggcgcggcg cgctttcgtc tgctcgacag acggggcggc gaccgtgcaa
      301 caccgcggcc tttgccggcc ctgccctacg ccgcggccgc cggcgtcgtc tcgcccagcc
      361 gcacgccgcg gacggcgtat tccgctgcca ccttgatcgc ctcgatcatg ctcaccgcac
      421 tggccttgcc ggtgccggcg atgtcgaagg ccgtgccatg atccacgctg gtgcgcagga
      481 tcggcagacc aagcgtgacg ctgaccgtct tctcgaaatc gagcatcttc gcggcgatgt
      541 gcccctggtc gtggtacagc gacagcaccg cgtcgtagcg gccgatcttc gccatgtgga
      601 acaccgaatc cgccggcacc ggcccgctga catgcaggcc gcgggcgcgc gccgccgcaa
      661 tcgcgggctt gatgccgtcg atctcctcgc ggccgaacag cccgtcttcg ccgccatgcg
      721 ggttcagcgc cgccaccgcc agatgcggct gctccaggcc aagctggcgt agcgccttga
      781 tgcagttgtc gatgcccttc tccacccgct cggcggtgat ggaacggcag gcgtccagca
      841 gcgacaggtg ccgggtgaga aagaagatct tcaacccgct gatggtgaac atcgtcatct
      901 cttcccgcgc cccggtatgt tcggcgaaca tctcggtatg gccgatgaag ggtatcttcg
      961 cggccttcag cgattccttg ttgatcggcg tggtggccac gccgtccacc cgtccggcca
     1021 tggccagatc aatcgcactg cggatcgccg cataggccgc ctgcccccca agcggctgca
     1081 ccttgcccat ctgcagcatg tccggatcgg cgctgtcctg gtgcagcaca tcgatcgtgc
     1141 cggcccggtc ggccagcgcc tccggacccg ggatgcggtt caatgcggcc gacagcccgc
     1201 acaccgacag ggcgcgctgg aacacccgcg cgtcgccgat cagaaaactg cgagacactg
     1261 cgcgcacctc cggatccagc agggcgcgga cggcgatctc tgggccgatg ccggcaggat
     1321 cgcccatcgt cacagccaac agcggccgct gcgtcatcga atatgccctc cagcgcgtgg
     1381 tccggcgcag tttgacccag ccgcggcgtt cccgccagcg cgaagcgcgt taacggtttg
     1441 ctcaggattt ccgcgcatca tggcgccgcg gaaaacccgc gccgcgccac ccagaacgag
     1501 gtctcgccgt gcgcctgctg ccgctgtgct gcgccatctt cgcgttgctc gccgggtgcg
     1561 cgcccggtca cggactgcgg ccgctgccgc ggatcgccgt cggcgactat cgcctgggac
     1621 cgggcgattc agtacgcctg acaacattcg gtgaggagca gctcaccggc gagttccggg
     1681 tcggcgcgaa cggcatggtc gcagtgccac tgctgggcga ggtgcgggcg gcagggctga
     1741 cgccgacgca actgcaagac gccgtagccg caggactgaa gcgcaggaac ctgttgcgcg
     1801 acccgaacgt ggccgcggag gtggtggaat atcggccgat ctttgtgctg ggggaggtga
     1861 acaagcccgg ccagtatccc tatcaaccgg ggatgaccat ggtgaccgcg gtcgccgtgg
     1921 ccggcgggtt cacctatcgc gcggtgcagg gctatgcttc ggtggtgcgg acggtggacg
     1981 gcgcggccga ggagcggaaa gcctcccgcg atgccttcgt gcagccgggc gacgtgctga
     2041 cggtgttcga acggcggttc tgaccaccaa cgtcgccggc cagggaacca gccgcttgca
     2101 tggccagacg ccaccgcccg acacattccc ttgggttcat ccgttcaggt cggcgaaacg
     2161 cttgccggcg gcggccattt cgcgcagcag gggggcgggt tcccagccgg ccccgctggc
     2221 gctgtacagg gcctccaccc ggcgcagcat ttccgcaatg ccgaccgcgt cggcctcatg
     2281 caacggaccg ccgcgccagg cgggaaagcc atagccgtgc accagcacca cgtcgatgtc
     2341 ggacggccgc gcggcgatgc cgtcggccaa cacgcgggct gcctcgttca ccatggcggc
     2401 gtgcacgcgc tgctggacct cctcggcgct gatcgcccgc cgggcaatgc cgcgcagcgc
     2461 cgaggcgcgt tccgccatgg ccaccacctc gggatcggcg atccgcttgc cggcttcgtg
     2521 gatatagaat ccccggccgg tcttttggcc gtaatgtccc tgctcgcaga tccagtcgaa
     2581 caccggcacg tagcgctcgc gcgggtcgcg ggtccccgcc ttgcgctggc ggtcacgcca
     2641 gtccacgtcc agcccggcca ggtcgaagac cgcgaagatc cccatcgcca tgccgtaggc
     2701 ttccagcgcg gcatccacct gcgccggcgt cgcaccgtcc tccagcgcgt attctgcctg
     2761 ggcgcgccat tttcccagaa tgcgattgcc aatgaaacca tcgcatacgc cggtgaccac
     2821 cggttgcttg cccagaagct gcacggtggc cagcgcggtg gccagggcct ccggccacag
     2881 ccggcgcgtg cgcaccacct ccaccagccg catgacatgc gccgggctga agaagtgcag
     2941 gccgagcacc cgctccgggg catcgacctc atccgccagc aggtcgatgt cgagatagga
     3001 cgtgttgctg gccagcagcg tatcgcgccg caccatgccg gacagggcgc ggaacacgcg
     3061 gcgcttttcg tccagatcct ccaccaccgc ctcgattacc agatcggcgt ggcgcagcgc
     3121 ccgcagttcc gtggcgtagg cgatgcggcc ggtgcgttcc cggtacagcc gctcggccag
     3181 ccggccgctg cttagttggc ggtcgtagat ggcccgcacc cggacgcggg cggcggcttc
     3241 cgcctccgaa tcgcgttcca ccagcgtgac gtcgagcccg gcatccgcca gcgccacggc
     3301 gatgccggag cccatcgtgc cgccgccgac cacgcccacc tgccgcaccg gccagaccgc
     3361 ggcctgaacc ggcgcgcgcg cggcgacgcg ttcggcatgc gccaggtgcc gcagagcctt
     3421 ggtctgcggt cccaggcgca ggtcacgggc aagggcctcc tcccgccgca ccccgtcggt
     3481 cagcggcagg tcgatggcga gg
//
LOCUS       DASSZH010000073         4783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1672442, whole genome shotgun sequence.
ACCESSION   DASSZH010000073 DASSZH010000000
VERSION     DASSZH010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672442"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1839
                     /gene="pbpC"
                     /locus_tag="VFN42_01455"
     CDS             <1..1839
                     /gene="pbpC"
                     /locus_tag="VFN42_01455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HET8995311.1"
                     /translation="LGHDGTPVAFYPDARGEWKFATHPNTVDPLLVRMLIAIEDHRFY
                     RDPGFDPLAIVRAGAQLLIAGHVVSGASTLTMQIARLLHPAPRTLPVKLREAMQALSL
                     TEHYSKQRIMGMWLSLAPFGGNIVGVQAASERWFGKPPRSLSPAQAALLIALCRRPEA
                     LRPDRHPEAARIARNRVLAQAAIDGVISPEEAARAAAEPVPTRWQALPRQSPQATLRL
                     ADGTRTTLDAPLNAALARLASQIVTTLHARESLAILIVDARTRAIRAAYLGDFADPAR
                     AGFVDLSRAVRSPGSALKPFLYGLAFADGLAGPDTRLRDLPRDFAGYGPDDFTHQFMG
                     TVTAAAALRRSLNLPAVALMQRLGPARFTASLAAAGVPLQLPSGAAPSLPIALGGAGI
                     SMRQLVALYAGLATDGRVAPLHLLAGVRHRSAALLSLPVAQEIADILTRPLPGETATG
                     IAWKTGTSAGNRDDWAVGFDRRHVVAVWIGRPDGGALPGGAAVDRAVPVLAQAFGLLR
                     IRPRQAAPPARPLSLAMAPAGTPLALAAPPPQATLTTRVPIRLRVIGGVRPLTFLIDG
                     VKLDSIPALRSTSFQPPGPGFYRLRVIDGDGQSIESRLRVVGAPHG"
     gene            1957..2310
                     /locus_tag="VFN42_01460"
     CDS             1957..2310
                     /locus_tag="VFN42_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995312.1"
                     /translation="MVESRLYCPEEEFTGRLRSRLPMNVLFTFSAEQLQALRTAFGTR
                     FERRHSVDVRWRVRLPWQRYYVVVQCGRDKRVDPRRAQGQGSMAWGMGWLLTLAAGMA
                     GLGWLVMSVVPNLLR"
     gene            2418..3284
                     /locus_tag="VFN42_01465"
     CDS             2418..3284
                     /locus_tag="VFN42_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET8995313.1"
                     /translation="MFKYILVPVSGGEADASVLRGAAILARRSGGHLAFLHVRPDVAN
                     LAAAMAITAFADAAWNGGYLDALQQDVTERQARAKAALERFCTTEGIVFSGVSTTAGV
                     TAAWHAERGEVADCLARFGRTADLLVLGRPGDTTWAPADLLDTALLTVGRPLLIVPPR
                     PPAQLGEVVAIAWKETAEAAHAITAALPLLETARRVVILSVTEQASADQESCERLRQA
                     LVWHNLATTVQHLAPEGLEPADRLLNAAVRIGADLLVMGGYGHSRMREVAFGGFTRHV
                     LHAAELPVLMAH"
     gene            complement(3232..3825)
                     /locus_tag="VFN42_01470"
     CDS             complement(3232..3825)
                     /locus_tag="VFN42_01470"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HET8995314.1"
                     /translation="MPHRHCIPRHIGIVLTLLCLAFAGSVHAAAIHPRPAAVLRGSQT
                     HLLLPRFASIRAPVANLRRGPGLRYPIAWVYRRRHLPVLILREFGNWRLLRLPDGTRG
                     WMSRLLLTPHRSFVVIAPYAILRGAPRPKAAALARLRRGVVGVLRGCKASTLWCRVST
                     HGYSGFVRRTQIWGSDSDARINAPSAPAAPRRAARDG"
     gene            4178..4381
                     /locus_tag="VFN42_01475"
     CDS             4178..4381
                     /locus_tag="VFN42_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaB family protein"
                     /protein_id="HET8995315.1"
                     /translation="MPYASNDDLPPAVRDHLPEHAQDIFREAFNHAWQQHAGDEQTAF
                     RIAWGAVKRHYRKQDDRWVPLGR"
     gene            4406..>4783
                     /locus_tag="VFN42_01480"
     CDS             4406..>4783
                     /locus_tag="VFN42_01480"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8995316.1"
                     /translation="MANCVLLHGAYQGGWIWQRVATRLRAAGHAVYSPTMDGCAERRH
                     QLRAGIDTSTYVSEIAELLFYEDLCDVVLVGTSSGGMVVCGAAEKMRHRVARLVLVDA
                     LALFNGERTRDIVRRTTPVPPGLA"
BASE COUNT          695 a         1613 c         1738 g          737 t
ORIGIN      
        1 ctggggcacg acggcacgcc cgtcgccttc tatccggacg cacgcggcga gtggaaattc
       61 gccacccatc cgaacacggt cgatccgctg ctggtccgca tgctgatcgc catcgaggac
      121 catcgtttct accgcgatcc cggcttcgat ccgctggcca tcgtccgcgc cggcgcgcag
      181 ttgctgatcg ccgggcatgt cgtgtccggc gcctccaccc tgaccatgca gatcgcccgg
      241 ctgctgcatc cggctccgcg cacgttgccg gtgaagctgc gggaggcgat gcaggcgctg
      301 tcgctgaccg agcactattc caagcagcgc atcatgggca tgtggctgtc tctggcgccg
      361 ttcggcggca acatcgtcgg cgtgcaggcg gcatcggagc ggtggttcgg caagccaccg
      421 cgcagcctca gcccggccca ggcggcgttg ttgatcgcgc tgtgccggcg gccggaggcg
      481 ctgcggcccg accggcatcc ggaggcggca cggatcgccc gcaaccgcgt gctggcgcag
      541 gcggcgattg atggcgtgat ctctcccgag gaggcggcgc gcgccgccgc cgaaccggtg
      601 ccgacccgct ggcaggcact gccgcggcaa agcccgcagg ccacgctgcg gctggcggac
      661 ggcacgcgca ccacgttgga tgcgccgctg aacgccgcgc tggcgcgact cgccagccag
      721 atcgtgacga cgctgcacgc ccgcgagtcg ctggcgatcc tgattgtcga tgcccgcacc
      781 cgcgccatcc gcgcggccta tctcggcgat tttgccgatc cggcgcgcgc cggcttcgtc
      841 gatctgtcgc gcgcggtccg ctcgcccgga tcggcgctga aaccgtttct ctacgggctg
      901 gccttcgccg atggcctggc gggacccgat acgcggctgc gcgacctgcc gcgggatttc
      961 gccggctacg gaccggacga cttcacgcat cagttcatgg gcacggtgac cgccgcggcg
     1021 gcgctgcggc ggtcgctgaa cctgccggcg gtggcgctga tgcagcgcct cggcccggca
     1081 cgattcacgg caagcctcgc cgccgcgggc gtgccgctgc agctgccgag cggcgccgcg
     1141 cccagcctgc ccatcgcgct cggcggcgcc gggatcagca tgcgccaact cgtcgcgctg
     1201 tatgcggggc tggccacaga cggcagggtc gcgccgctgc acctgctcgc cggcgttcgc
     1261 catcggagcg ccgcgctgct gagcctgcct gtcgcacagg agatcgcgga tatcctcacg
     1321 cgtccgctgc cgggcgagac cgcgaccggc atcgcctgga aaaccggcac cagcgccggc
     1381 aaccgcgacg actgggcggt gggattcgat cgccggcacg tggtcgcggt ctggatcggg
     1441 cgcccggatg gcggcgcgct gcccggcggg gcggctgtgg accgggcggt gccggttctc
     1501 gcccaggcgt tcggcctgct gcggatccgg ccgcgccagg ccgcgccgcc ggccaggccg
     1561 ctgagcctgg cgatggcgcc ggccggcacg ccgctggcac tggcggcgcc accaccgcag
     1621 gcgacgctga ccacacgggt gccgattcgg ctgcgcgtga tcggcggcgt gcgccccctg
     1681 accttcctga tcgatggcgt gaagctggac agcatccccg ccttgcgcag cacgtcattc
     1741 caaccgcccg ggcccggctt ttatcgcctg cgcgtgattg acggagacgg gcagtcgatc
     1801 gagagccggt tgcgcgtggt gggggcgccg cacgggtgag cggacccaac ctcacgtcat
     1861 accaccgcta aaacatcgct atcgcagggg gtcgtttgaa accgaattgt aagttctctt
     1921 gcggcaaggt gacgccgcca tgggggagag catgggatgg ttgaatcgcg actgtactgc
     1981 ccggaggagg aatttaccgg gcgtctgcgc tcccgtctgc cgatgaatgt gctgttcacg
     2041 ttcagtgcgg agcaattgca ggctctgcgc acggcattcg ggacgcgttt tgagcggcgg
     2101 cacagcgtcg atgtgcggtg gcgggtgcgc ctgccgtggc agcgctacta cgtcgtggtg
     2161 caatgcgggc gtgacaagcg cgttgatccg cggcgggcgc aggggcaggg ctcgatggcc
     2221 tggggcatgg ggtggctgct gaccctggcc gcgggcatgg ccgggctggg ctggctggtg
     2281 atgtcggtgg ttcccaacct gctgcggtga gactggcaag gcccggcatg ccgggccgcg
     2341 cgccggaccg gcgttgattc aggtcaagga agttgcaggc caccgcggca atgtgtgcgc
     2401 ccaggtggga gactgcgatg ttcaagtaca tcctggtgcc ggtttccggc ggggaagcgg
     2461 atgcttccgt gctgcgcggc gcggccatcc tggcgcggcg gtccggcggt catctcgcgt
     2521 tcctgcatgt ccgcccggac gtggcgaacc tggccgcagc catggcgatc acggcgttcg
     2581 ccgacgcggc ctggaacggt gggtatctcg atgccttgca gcaggacgtg acggaacgtc
     2641 aggcacgcgc caaggccgcg ctggaacgct tctgcactac cgaggggatc gttttctccg
     2701 gcgtctccac caccgccggc gtcacggcgg cgtggcacgc ggagcgtggg gaagtcgcgg
     2761 attgcctggc gcgattcggc cggacggccg acctgctggt gctgggaaga ccgggcgaca
     2821 cgacctgggc gccggccgac ctgctggata cggcgctgct gaccgtcggg cggccgctgc
     2881 tcatcgttcc accgcgccct ccggcacaac tgggagaggt ggtcgcgatc gcctggaagg
     2941 aaaccgccga ggcggcgcac gcgatcaccg cggcgttgcc cctgctggaa accgctcggc
     3001 gcgtggtgat cctgtcagtg accgagcagg catccgccga ccaggagtcc tgtgagcgcc
     3061 tgcgtcaggc gctggtctgg cacaatctgg cgaccaccgt gcagcatctg gcgccggagg
     3121 gcctggaacc agccgacagg ctgctgaatg ccgcggtgcg gatcggcgcc gacctgctgg
     3181 tgatgggcgg ctatggccac agccggatgc gggaagtcgc gttcggcggc ttcacccgtc
     3241 acgtgctgca cgccgcggag ctgccggtgc tgatggcgca ttgatgcgcg catcgctatc
     3301 gctgccccag atttgcgtcc gccgcacgaa gccggaatag ccgtgcgtgc tgacgcggca
     3361 ccacagggtg ctcgccttgc atccgcgcag cacgcccacc acgccgcggc gcagccgcgc
     3421 cagcgcggcg gccttcgggc gcggggcgcc gcgcaggatg gcatagggcg cgatcaccac
     3481 gaagctgcgg tgcggtgtca gcagcaggcg ggacatccag ccgcgtgttc catccggcag
     3541 ccgcagcagg cgccagttgc cgaactcccg caggatcagc acgggcagat gccggcggcg
     3601 gtacacccag gcgatcgggt aacgcagtcc ggggccgcgc cgcaggttgg cgacgggcgc
     3661 gcggatcgag gcaaagcgtg gcaacagcag gtgcgtctgc gacccgcgca ggaccgccgc
     3721 agggcgcggg tggattgcgg cggcatggac ggagccagcg aaagccaggc agagcagggt
     3781 cagcacaatc ccgatatgcc gcgggatgca gtggcggtgt ggcatgcgcg cgcagctatc
     3841 acgctggtca cggactcatc gtctgccgcg gggtgaaaaa tgggtcatcc ccaggtgccg
     3901 cgatcggcgc cggaatccgt ttgaccgccg gccggccagc gtcttagcgt cctgcgactt
     3961 gccgctgcga gcagtgcgac cccgcttccc ggggcgacgc tgcagggtaa tgtccgcacg
     4021 tgcagggccg aacacgacaa gccgaacaaa agacaagttc agggagcacg atggttcgtc
     4081 gctttttccc gtggcggacc cgaattcggg acggcgaccg ttcaaccgcc gcccgacatt
     4141 gcgccggcat ggcgcagccg gataggctga ctgcgccatg ccgtacgcca gcaatgacga
     4201 tctgcccccg gcggtgcgcg accacctgcc ggagcacgcg caggacatct tccgtgaggc
     4261 gttcaaccac gcctggcagc agcatgccgg tgacgagcag accgcgttcc gcatcgcctg
     4321 gggcgcggtg aagcggcatt atcgcaagca agacgatcgc tgggtgccgc tggggcggtg
     4381 acctgccggc aagggggaag caatcatggc gaactgcgtg ctgctgcacg gcgcctatca
     4441 gggcggctgg atctggcagc gtgtcgcgac acgcctgcgt gccgcaggtc acgccgtata
     4501 ttccccgacc atggatggct gtgccgagcg tcggcatcaa ctgcgtgccg gcatcgacac
     4561 atcgacctac gtctcggaaa tcgccgagct gctcttctac gaagatctgt gcgatgtcgt
     4621 gctggttggc accagctcgg gcggcatggt ggtttgcggc gcggcggaga agatgcggca
     4681 ccgggtcgca cgcctggtgc tggtcgatgc attggcgttg ttcaacggcg agcgcacgcg
     4741 tgacatcgtg aggcgcacga cccccgtccc gccgggactc gcc
//
LOCUS       DASSZH010000074         2204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_479354, whole genome shotgun sequence.
ACCESSION   DASSZH010000074 DASSZH010000000
VERSION     DASSZH010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479354"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1182)
                     /locus_tag="VFN42_01485"
     CDS             complement(28..1182)
                     /locus_tag="VFN42_01485"
                     /inference="COORDINATES: protein motif:HMM:NF004811.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HET8995317.1"
                     /translation="MPGLQRASAAIVGAAESDIGAVASLMSPIDLMAQGIHRALEDAG
                     LTLHDVDGLFCATTQARTSVMSLAEYLGLPDVYTDSTIVGGSSFEIHVAHAQAALQAG
                     LCSVAVIAYGSTQRTVGRRSASVREYNPYETPFRPFLPASAYALAASRHMHEFGTTRE
                     QLAEVAVAAREWALLNPAAWEKQPLTIAEVLNARPISDPFTVRDCCLVTDGGGAIVMT
                     TPARARDMARPPVFVLGCGQAITHAAISSMPDLTVTGAVESGAQAYAMAGLAPADIGV
                     LALYDAFTINTILFLEDLGFCPKGEGGRFVQDGRIGPSGDLAVNTNGGGLSYCHPGMY
                     GLFLLIEAVRQLRGECGARQVAAADTALVHGNGGVLSAQATVILGGASAL"
     gene            complement(1183..1566)
                     /locus_tag="VFN42_01490"
     CDS             complement(1183..1566)
                     /locus_tag="VFN42_01490"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HET8995318.1"
                     /translation="MPPDTASPLTTYIAHLERGELAYQFSPSADTAVFYPRVVAPRTG
                     AADLEWRISKGMGTVYATTVVHQPKGDPYNVVLIDMDEGYRLMSRVEDIAPSAVTIGM
                     RVKFRVHPAEGDEPPYPVFTPVENA"
     gene            1762..>2204
                     /locus_tag="VFN42_01495"
     CDS             1762..>2204
                     /locus_tag="VFN42_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004496.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HET8995319.1"
                     /translation="MSLKGKAYIVGAWEHPTRKATDKSVTLLHAECAKGALEDAGLTK
                     DDVDAYYCAGDAPGMGPMSMVDYMNLKCRHVDSTDVGGSSYQVAVGHALEAIAAGRVN
                     VALITLAGRPRSEGQATGTAPRAGNPAQADVQFEYPYGPATANMYA"
BASE COUNT          380 a          747 c          738 g          339 t
ORIGIN      
        1 cgcagcgccc gtcaagcacg cgccgcgtta cagggcagac gcaccgccga gaatcaccgt
       61 tgcctgcgcc gacagcacgc cgccattgcc gtgcaccaac gctgtatccg ccgcagcaac
      121 ctggcgcgca ccgcattcgc cacgcaactg gcgtaccgcc tcgatcagca ggaacaggcc
      181 gtacatgccg ggatggcagt atgacagccc gccgccattg gtattcaccg cgaggtcacc
      241 gctgggcccg atccggccgt cctgaacgaa gcggccgcct tcgcccttcg gacagaagcc
      301 caggtcttcc aggaacagga tggtgttgat ggtgaacgcg tcatacagcg ccagcacgcc
      361 gatatcggcc ggcgccagac ctgccatcgc gtaagcctgc gcgccggatt ccaccgcccc
      421 cgtcaccgtc agatccggca tcgacgagat cgccgcatgg gtgatcgcct ggccgcagcc
      481 gagcacgaag accggcggcc gggccatgtc gcgggcgcgg gccggcgtgg tcatcacgat
      541 ggcaccgccg ccgtcggtca ccaggcagca gtcgcggacg gtgaagggat cgctgatcgg
      601 ccgcgcgttc agcacctcgg cgatggtcag cggctgcttc tcccaggcgg ccggattcag
      661 cagcgcccat tcgcgcgcgg ccaccgcgac ttccgccaac tgctcgcgcg tagtgccaaa
      721 ttcatgcatg tggcgcgagg ccgcgagcgc ataggcgctg gccggcagga agggacggaa
      781 cggcgtctcg tacggattgt attcgcgcac gctggcggag cggcgtccga cagtgcgctg
      841 cgtgctgcca taagcgatca ccgccacgct gcacagcccg gcttgcagcg ccgcctgggc
      901 gtgcgccacg tggatctcga aggacgaacc gccaacgatg gtggaatcgg tgtagacgtc
      961 cggcaggccg agatattcgg ccagcgacat caccgaggtg cgcgcctggg tggtggcgca
     1021 gaacagccca tcgacatcat gcagtgtcag tcccgcatct tccagtgcgc gatggatgcc
     1081 ctgcgccatc aggtcgatcg gactcatcag cgacgcgacg gcgccgatat cggattccgc
     1141 cgcgccgaca atcgccgcgc tggcacgttg cagaccaggc attcaggcgt tctccaccgg
     1201 cgtgaacacg ggataaggcg gctcatcgcc ctcggccgga tggacgcgga acttcacccg
     1261 catgccgatc gtcaccgcgc tgggcgcgat gtcctccacg cggctcatca gccggtagcc
     1321 ttcatccatg tcgatcagca ccacattgta gggatcaccc tttggctgat gcaccaccgt
     1381 ggtggcgtag accgtgccca tgcccttgct gatccgccat tccagatcgg ccgcgccggt
     1441 ccgtggtgca acgacgcgcg gatagaacac tgccgtgtcg gcggacgggc tgaactggta
     1501 ggccagttcg ccgcgctcga gatgcgcgat gtaggtggtg agcggcgagg cagtatccgg
     1561 cggcattggc tgttccttcg cggtgccgcc cgcagaatag gccagggctg ccggattggc
     1621 gacgccgatg ccgcaggacc gagcctgcct cccggcggtt cgccggatgc gccttcccgg
     1681 ggcggctatg cggcgggcta ctgatcagta tactcctggc ccgatcagcc ggaccgggtt
     1741 cgatcgcaag gggagtgaag gatgagtttg aagggcaaag cctatatcgt gggggcttgg
     1801 gagcatccca cccgcaaggc gaccgacaaa tcggttaccc tgctgcacgc cgagtgcgcc
     1861 aagggcgcgc tggaagatgc cggcctgacc aaggatgacg tggacgccta ctactgtgcc
     1921 ggcgacgcgc ccggcatggg gccgatgtcc atggtcgact acatgaacct gaaatgccgg
     1981 cacgtggatt ccaccgatgt gggtggctcc tcgtaccagg tggcggtcgg gcacgcgctg
     2041 gaggcgatcg ccgccggccg cgtcaatgtc gcgctgatca cgctggccgg gcggccgcgc
     2101 agcgagggcc aggccaccgg caccgcgccg cgcgccggca atccggcgca ggccgacgtg
     2161 cagttcgaat atccgtacgg tccggccacc gcgaacatgt atgc
//
LOCUS       DASSZH010000075         2180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_2156, whole genome shotgun sequence.
ACCESSION   DASSZH010000075 DASSZH010000000
VERSION     DASSZH010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2156"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /gene="narI"
                     /locus_tag="VFN42_01500"
     CDS             <1..413
                     /gene="narI"
                     /locus_tag="VFN42_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HET8995320.1"
                     /translation="IRATSSFSDTAILLLLWLQLTLGLATIPVSAQHLDGSEMVKFMS
                     WAQGILTLHPGAAALIADVNPIFKLHLFLGMTIFLLFPFTRLVHIWSAPVWYFGRTGY
                     QVVRTRRHIAHRPAETAAAPMQAAVRPQPAMGDD"
     gene            417..1238
                     /locus_tag="VFN42_01505"
     CDS             417..1238
                     /locus_tag="VFN42_01505"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET8995321.1"
                     /translation="MRTVLTRSTTTPPVAVNGAVIANADIAREVQNHAEATPKQAWQE
                     ATRALVVRELLLQRAHALGLAAAPRSEDGLRETEEEGLIRALLESEVHTPTAGEAECR
                     RYYDANQTKFRNPTLFEPQHILFKAPREDAAAYAAALMRAEAALAEVQSAPGLFTALA
                     RALSDCPSSGDGGRLGQVSRGETTPEFETALVSLQAGETCPHPVQTRYGVHVLRLERK
                     TASETLPFELVRERVAAYLEEHAWRRAVAQYVALLAGQACISGFAMPSAPSPLVQ"
     gene            1250..1552
                     /locus_tag="VFN42_01510"
     CDS             1250..1552
                     /locus_tag="VFN42_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995322.1"
                     /translation="MLGDILHDLTDPAMAEATLALLNEPALRDRITGVAAAQSRPVGA
                     LIAGKIRHLLDHGDETLWLDLLTAMSASPQPGAAAVTRILALAFPDPVRVRITHQA"
     gene            1549..1962
                     /locus_tag="VFN42_01515"
     CDS             1549..1962
                     /locus_tag="VFN42_01515"
                     /inference="COORDINATES: protein motif:HMM:NF023184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HET8995323.1"
                     /translation="MSGGATPVESGTDFVRRGREFRTIAAMLRMYCRTHHAPTAAALC
                     PACTELHDYAKRRLERCVFGEAKPTCANCTVHCYKASMRERIRQVMAWAGPRMLWRHP
                     VLAIRHLIDGRRLAPTLARPAPQTTRQSRRAGDGI"
     gene            complement(1977..>2180)
                     /locus_tag="VFN42_01520"
     CDS             complement(1977..>2180)
                     /locus_tag="VFN42_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HET8995324.1"
                     /translation="LTGLPDNAVEPVLLVECPRILFPFARNILADVTRDGGFPPVLLQ
                     PIDFVALWQSRRQQQQAPAGTTA"
BASE COUNT          378 a          751 c          708 g          343 t
ORIGIN      
        1 gcatccgcgc cacctccagc ttcagcgaca ccgccatcct gctgttgctg tggctgcagc
       61 tgacgctcgg cctggcgacc attcccgtgt cggcgcagca cctggacggc agcgagatgg
      121 tgaagttcat gagctgggcg cagggaatcc tgactctgca tccgggtgcg gccgcgttga
      181 tcgccgacgt gaacccgatc ttcaagctgc acctgttcct ggggatgacg atcttcctgc
      241 tgttcccctt cacccgcctg gtgcacatct ggagcgcgcc ggtctggtat ttcggccgca
      301 ccggctatca ggtcgtacgc accaggcgcc acatcgcgca ccgtccggct gagacggctg
      361 ctgctccgat gcaggccgca gtgcgcccgc agccggcgat gggagacgac tgaaccatgc
      421 gcacagtcct gacccgcagc accaccacac cacccgtcgc ggtcaacggc gcagtcatcg
      481 ccaacgccga catagcgcgc gaggtgcaga accacgccga agcgacaccc aaacaagcct
      541 ggcaggaagc aacccgcgca ctcgtcgtgc gcgaactcct gctgcaacgg gcgcacgccc
      601 ttggtctggc cgcagcaccc cgcagtgaag atggattgcg cgaaaccgag gaagaggggt
      661 tgatccgcgc cctgctggaa tccgaggtgc acacgcccac cgctggcgaa gccgaatgcc
      721 gccgctacta cgatgcgaac cagacgaagt tccgcaatcc gacattgttt gaaccgcagc
      781 acatcctttt caaggcgccg cgtgaagatg ctgcggccta cgcagcggcg ctgatgcggg
      841 ccgaagcggc gcttgcggag gtgcaatccg cgccgggcct cttcaccgcg ttggcccgtg
      901 cgctctcgga ttgcccatcc tccggcgatg gcgggcgtct tggccaggtc tcgcgcggtg
      961 aaaccacacc cgagttcgaa accgctctgg tatccttgca ggcaggcgaa acctgcccgc
     1021 atccggtgca gacaagatac ggcgtgcatg tgctgcgcct ggaacgcaag accgcatccg
     1081 agacactgcc gttcgaactg gttcgcgaac gcgtcgctgc gtatcttgag gaacacgcct
     1141 ggcgccgcgc cgtcgcacaa tacgtggcgc tgctggccgg tcaggcatgc atcagcggat
     1201 tcgcgatgcc gtccgcccca tcgccattgg tgcagtaagg aggaacgcga tgctgggcga
     1261 catcctgcac gatctcaccg acccggctat ggcggaagca accctggcgc tgttgaacga
     1321 acccgcgctg cgtgaccgca tcaccggggt ggcggccgcg caatcgcggc cggttggcgc
     1381 actgatcgcc ggcaagatcc gtcacttgct tgaccatggc gacgaaacgc tatggcttga
     1441 tctgctcacc gccatgtccg cgtcgccgca gcccggggcc gccgcggtca cgcgcatcct
     1501 ggcgctcgcg tttcccgacc cggtgcgggt ccgcatcacg caccaggcat gagcggcgga
     1561 gccacgccgg tggagtccgg caccgacttc gtccggcgag gccgcgaatt ccgcacaatt
     1621 gccgcgatgc tgcgcatgta ctgtcgtacg catcacgcgc cgactgcggc ggcgctctgc
     1681 cccgcatgca ccgagctaca tgattatgcc aagcggcggc tggagcgctg cgtgttcggc
     1741 gaagcgaaac cgacctgcgc caactgcact gtgcactgct acaaggccag catgcgcgag
     1801 cggatccgcc aggtgatggc ctgggccggc ccacgcatgc tctggcggca tccggtgctt
     1861 gcgattcggc atctgatcga cggcaggcgg ctggcgccca cgcttgcaag gcccgctccc
     1921 cagacgacgc gacaatcccg ccgcgccggc gacgggatat gacgccgtga aactgctcag
     1981 gccgtggtgc cggccggcgc ctgctgctgc tggcggcggg actgccacag cgccacgaaa
     2041 tcgatcggtt gcagcaacac ggggggaaag ccgccgtcgc gggtcacgtc ggccaggatg
     2101 ttgcgggcga aggggaacag gatgcggggg cattcgacga gcagtaccgg ctcgaccgcg
     2161 ttgtcgggca ggccggtcag
//
LOCUS       DASSZH010000076         3187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_683819, whole genome shotgun sequence.
ACCESSION   DASSZH010000076 DASSZH010000000
VERSION     DASSZH010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683819"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VFN42_01525"
     CDS             complement(<1..734)
                     /locus_tag="VFN42_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HET8995325.1"
                     /translation="MNTTATTPDNEPPNRPLGDFTTNPRVLIISAIALVVATAGVIAG
                     VVLLNLIRLCTNLAYFGSVSLADLKLGASPWGAAAIGIPVIGGLIVGLMARYGTDKIR
                     GHGIPEAIEAILLGRSRLDVKVAILKPLSSAIAIGTGGPFGAEGPIIMTGGAVGSLIA
                     QMLPVSDSERKTLLVAGAAAGMTTVFGTPIAAIMLAVELLLFEWTPRSFVPVAAAAIL
                     ADVERTWLGLPTPLFPFHGGMPTSFEA"
     gene            1005..1652
                     /locus_tag="VFN42_01530"
     CDS             1005..1652
                     /locus_tag="VFN42_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET8995326.1"
                     /translation="MKQAKLAAAVLTGLMLAATGQANATVVSILFSGPGVSGSLAVTY
                     GTATDATYSNAYEVTGISGTFSDSNNGLGISNAAVTGLVAVTHATPESGNLLAPNDFS
                     RYAVTSGLPSANNGYLTYDNLYWPDGSPQTATDYPVSGGILDIYGLMFTINGSEVVDF
                     WSNGGSSPDYGVAVATSFEALDYVSSGVEVPEPGSFALLASGVLGMLAWRRRKVA"
     gene            complement(1781..1957)
                     /locus_tag="VFN42_01535"
     CDS             complement(1781..1957)
                     /locus_tag="VFN42_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995327.1"
                     /translation="MKRFSYSGEDTPDNQRAFPQKTSDALKSSDEELAKGGLTISAMT
                     LVAPDFLKAMTATS"
     gene            complement(2355..3083)
                     /locus_tag="VFN42_01540"
     CDS             complement(2355..3083)
                     /locus_tag="VFN42_01540"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET8995328.1"
                     /translation="MVGIIMVLAALLGGCSAPTGSGPSATTFRDILRPDVTGRSPAVV
                     LLHGCSGVQDYHYDWARTLRGWGYVVLVDDSFTARGLTTVCGVGGARVTPTTMADDAF
                     AALDHLRSLPFVDPARIGVIGFSFGGGAALEVASRGFQDTHHPGKPGFRASVAFYPLC
                     EVRHDIAIPLLILQGDADDLASPAANCTARVKDLAGQGKPVRITVFPGAGHAFDDMRN
                     PNYDSAAERQAEALVRAFLAKSLQ"
BASE COUNT          532 a         1009 c         1099 g          547 t
ORIGIN      
        1 gcctcgaagg aggtgggcat gccgccgtga aacgggaaca gcggcgtcgg cagaccgagc
       61 caggtgcgct cgacgtcggc aaggattgcg gccgcggcga ccggcacgaa gctgcgcggg
      121 gtccattcga acagcaatag ctccaccgcc agcatgatcg cagcgatcgg cgtgccgaac
      181 accgtggtca tgccggctgc ggcgccagcc accagcagtg tcttccgttc gctgtcgctg
      241 accggcagca tctgggcgat cagcgagccg accgcgccac cggtcatgat gatcggacct
      301 tccgcgccga acgggccgcc ggtgccgatg gcaatggccg aggacagcgg cttcagaatc
      361 gccaccttga cgtccagccg gctgcggccg agcaggatgg cctcgatcgc ttcgggaatg
      421 ccgtggccgc ggatcttgtc ggtcccgtag cgtgccatca gcccgacgat caggccgccg
      481 atcaccggaa tcccgatggc ggcggcaccc cagggcgagg cgcccagctt gaggtcggcc
      541 aggctcacgc tgccgaaata ggccaggttg gtgcagagcc ggatcaggtt cagcagcacg
      601 acaccggcga tgacgccggc cgtggcgacc accagggcaa tggcggaaat gatcagcacg
      661 cggggattgg tggtgaagtc gcccagcggg cggttcggcg gttcgttgtc cggggtggtg
      721 gcggtggtgt tcacggggtg cctggctgcg gtgtggggcg tcgcggcgga atacatcgtt
      781 atgcgatata ccggcaagcc gccccggttg cctcgattcg gataccgcgc gccagacggc
      841 gggccggccc gagcaacgtc cggttgggaa ggcgccgcga aggccctggg cgacaggtct
      901 ggtgggttgc cgtatcgacc ctgtagtgca accatgccgg gaaggccggc attcaagcgg
      961 ataagcccat ccacttcgat gcgcgcgcca ccaggggaag ttccatgaag caagccaaat
     1021 tggcggctgc tgttctgacc ggtctgatgc tggccgcgac cggtcaggcc aatgccacag
     1081 tcgtatccat cctgttcagt ggaccgggcg tcagcgggtc gctggcggtg acctatggca
     1141 cggcgacgga cgccacgtac tccaacgcct atgaagtcac cgggatcagc ggcacgttct
     1201 ccgattcgaa caacggcctg ggtatctcta atgcggcggt gaccgggctg gtcgccgtca
     1261 cccacgccac gccggaatcg ggcaatcttc tggcgccgaa cgatttcagc cggtacgcgg
     1321 tcaccagcgg gctgccgtcc gccaacaacg gctacctgac ctatgacaac ctgtactggc
     1381 ccgacggctc gccgcagacc gccacggatt atccggtttc cggcggcatt ctggatatct
     1441 acggactgat gttcaccatc aacggcagcg aggtggtcga tttctggagt aacggcggta
     1501 gctcgcccga ttacggcgtt gcggtggcca cgtcgttcga agcgctggat tatgtcagca
     1561 gcggcgtcga ggtgccggag ccgggttcgt ttgcgctgct ggcgagtggc gtgctgggca
     1621 tgctggcctg gcgccgccgc aaggttgcct gacaccccgg atcgccccag cgagcgatct
     1681 tcatggccgc ggggctgccg gagatggcag cccatgtcgg tccgctgatg ctgtcgctgc
     1741 aggatacgaa cccgctgggt gcgactgcgc ggcgcgcctt ctatgacgtt gcggtcatcg
     1801 ccttcaggaa atccggcgcg accagcgtca tcgcggagat cgtcaggccg cctttggcga
     1861 gttcttcatc gctgctcttg agcgcatcgg acgtcttctg cgggaacgcg cgttggttgt
     1921 ctggcgtgtc ctcgcctgaa tagctgaaac gcttcaaggc atcgtcgggc catcaacggc
     1981 ccggccttct ctctgccggg cgaattcctt ctgcctgccg agcttctcct ccggtgccat
     2041 ggtgtggggg gcggccaaga tcgtttctcc tcagcgcccg cggtccggcg tcagcgggcc
     2101 atctcgatcc ggtcgagcgg gtgcacggtt ttcgtgttgc tgtagtggat cggtgcatgc
     2161 ggggcaccaa cccaagccct gccaggatat ccggcagcag ttcccggagg tgaaggtcgg
     2221 gacacggcct ttatcatcgg ccgcagcttt gccggcgttc gtcatcggta ctgatgacgt
     2281 catttcatcg cacgctaatg gacgatggat gacggcgtaa ggagaagatt ggtcgcggcg
     2341 cagtctccgc gctcttattg caaactcttg gcaagaaatg cgcgcaccag cgcttccgcc
     2401 tgacgctccg ccgcggagtc gtaattcggg ttccgcatgt cgtcgaaggc atgacccgcg
     2461 ccgggaaata ccgtgatgcg gacaggcttc ccctgaccgg ccaagtcctt gacccgggcg
     2521 gtgcagttgg ccgccggcga ggcgagatca tccgcatcgc cctgcaggat cagcagcggg
     2581 atggcgatat cgtggcgcac ttcgcacagc ggatagaatg ccaccgaagc ccggaagccg
     2641 ggcttgcccg gatgatgcgt gtcctgaaat ccgcggcttg cgacctcgag cgcggcaccg
     2701 ccgccgaacg agaagccgat gacgccgatg cgcgccggat cgacgaatgg caaggagcgc
     2761 aagtggtcca gcgcagcgaa tgcgtcgtct gccatggtgg tgggggttac ccgcgcaccg
     2821 ccgacaccgc agacggtggt caggccgcgc gcggtaaagc tgtcgtcgac cagaaccaca
     2881 taaccccacc cgcgcagggt gcgcgcccaa tcgtagtgat agtcctgcac gccgctgcag
     2941 ccgtgcagca ggaccaccgc cggcgatcgg ccggtgacat cgggccgcag gatgtcacgg
     3001 aacgtggtcg cggacgggcc ggaaccggtg ggcgccgagc agccgccgag cagggcggcg
     3061 aggaccatga tgatgcccac cacgccggga taccggtggg ctgcgccagc gttgcggctg
     3121 ggtggctttg ccgcaagcgc gccggctcga tgcctgctgc tgtgcatggg gcggcgcctc
     3181 gttcaat
//
LOCUS       DASSZH010000077         2573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_104310, whole genome shotgun sequence.
ACCESSION   DASSZH010000077 DASSZH010000000
VERSION     DASSZH010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104310"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            339..1049
                     /gene="mtgA"
                     /locus_tag="VFN42_01545"
     CDS             339..1049
                     /gene="mtgA"
                     /locus_tag="VFN42_01545"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HET8995329.1"
                     /translation="MPGRRSAAPGARSIRLWRLARRLLLVLLLGPPLLILLFRFVPVP
                     LTPLMVIRLVQGYGLHHQWVGYERIAPDLPRSLIASEDNLFCQEKLGFDTAALETQIG
                     VWWHGGRPRGASTITMQVARNLFLWQGRDWARKLLEAWLTPQVALLWPKQRILEVYLN
                     SVEFGPGLFGVQAAARQDFRRSAAQLDETQAARLIAVLPAPLEWSPAHPNRLVLWRAD
                     IIRHRIGQLGPLLDCARR"
     gene            1233..2540
                     /gene="gltA"
                     /locus_tag="VFN42_01550"
     CDS             1233..2540
                     /gene="gltA"
                     /locus_tag="VFN42_01550"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HET8995330.1"
                     /translation="MSATAKRSVTVTLDGTNKSTSLPLISGTAGADVFDIRRLHGDLG
                     VFTYDPGYGATASCESKITYIDGDAGVLQYRGYPIEQLAEHSHFLEVCYLLLNGELPN
                     AKQMEDFETGVLRHTMLHEQIRSFYNGFRRDAHPMAVMCGVVGALSAFYHDSLDIYDP
                     ESRRISAFRLIAKVPTIAAWAHKYSVGQPFMYPRNDLGYAENFLYMFHAVPTETWKAN
                     PILSRAMDLIMILHADHEQNASTSTVRLAGSTGANPFACIAAGIASLWGPAHGGANEA
                     VLKMLAEIGHVKNIPAFLEDVKDRNNHAKLMGFGHRVYKNYDPRARIIQKACHDVLGE
                     LGIKDDPLLDLAMELEKIALNDKYFVDRKLYPNVDFYSGIILKAMGFPTSMFTALFAV
                     SRTVGWVSQWQELMTDPEQRIGRPRQIYTGPAQRDYVAVGKRG"
BASE COUNT          429 a          875 c          849 g          420 t
ORIGIN      
        1 gcaggaagag ggaggcgatc gaaccagtca gcatcgtgcg tcgcctgcga acctctgata
       61 agtggttgtt ggggatgaca tggcgaccgg gaggtgatgc gcaccatcac cgttctgtgt
      121 tgcagtcacg cttgtaaccc aaagtgctgc gggagccacc ttatgccttc gtcacaatcg
      181 gaacgcgccg ggcggcccgg cgtcgcaccg gggcagcggc tgctccggct gcctctggca
      241 ccgcggcggc ggaatacagg gtcgcgcgga gctgcgcgga cccggaccac gcggccggcc
      301 tgagccgcgc ggcggcgatc ctggcttcct cggcacgttt gccgggccgc cgttcggcgg
      361 cacccggcgc caggagcatc cggctctggc ggctcgcccg ccgtctgctg ctggtcctgc
      421 tgctgggccc gccgttgctg atcctgctgt tccgcttcgt cccggtgccg ctcacgcctt
      481 tgatggtgat ccgcctggtg cagggctacg gcctgcacca ccaatgggtc ggttatgagc
      541 gcatcgcgcc ggacctgccg cgcagcctga tcgcctcgga ggacaacctg ttctgccagg
      601 agaagttggg cttcgatacc gccgcgctgg aaacgcagat cggtgtctgg tggcatggcg
      661 gccggccgcg cggggccagc accatcacca tgcaggtcgc ccgcaacctg ttcctgtggc
      721 aagggcggga ttgggcccgc aagctgctgg aagcctggct cacgccgcag gtcgccctgc
      781 tatggccgaa gcagcggatc ctggaggtct acctgaacag cgtcgaattc ggccccggcc
      841 tgttcggcgt gcaggccgcc gcccggcagg atttccggcg ctccgcggcg cagctcgacg
      901 agacccaggc cgcccggttg atcgcggtgc tgccggcgcc gctggaatgg tcgccggcac
      961 accccaaccg gctggtgctg tggcgggccg atataatccg ccaccgaatc ggacagcttg
     1021 gcccgctgct ggactgcgcc cgccggtgag cgtagctagc cgcaggccga gggcggagcg
     1081 cggatttttg ctgcgggcgc gaaggttgcg tttcggcccg acgcgaccat aatcccgcct
     1141 tattccgcac cggccgccgc gccggcggag cgaggtcgca cgaggcatcc gaaacggcgg
     1201 cgtccgaccg cccctgtcac aaggacacgc gcatgagcgc aactgccaaa cggtccgtca
     1261 ccgtcaccct ggacggaacc aacaaatcga ccagcctgcc gctgatctcc ggcaccgcgg
     1321 gcgcggacgt tttcgacatc cggcgtctgc acggcgacct cggcgtgttc acctacgacc
     1381 cgggatacgg cgccaccgcg tcctgcgaga gcaagatcac ctatatcgat ggcgatgccg
     1441 gagtactgca gtaccgcggc tacccgatcg agcaactggc cgagcactcg catttcctgg
     1501 aagtctgcta cctgctgctg aacggcgaac tgccaaacgc caagcagatg gaggacttcg
     1561 agaccggcgt gctgcggcac accatgctgc atgagcagat ccgcagcttc tacaacggtt
     1621 tccgccgtga cgcgcacccg atggcggtga tgtgcggcgt ggtcggcgcg ctgtcggcgt
     1681 tctatcacga cagcctggac atctacgatc cggagagccg gcggatcagc gcgttccggc
     1741 tgatcgccaa ggtgccgacg atcgccgcct gggcgcacaa atactccgtc ggccagccct
     1801 tcatgtatcc gcgcaacgac ctcggctacg ccgagaactt cctgtacatg ttccacgcgg
     1861 tgccgaccga gacctggaag gccaacccga tcctgtcgcg cgcgatggac ctgatcatga
     1921 tcctgcacgc ggaccatgag cagaatgcct ccaccagcac ggtgcggctg gcaggttcca
     1981 ccggcgccaa tcccttcgcc tgcatcgccg ccggcattgc cagtctgtgg ggtcccgcgc
     2041 atggcggcgc caacgaggcg gtgctgaaga tgctggccga gatcggccac gtgaagaaca
     2101 tccccgcctt cctggaggat gtgaaggacc gcaacaacca cgccaagctg atgggcttcg
     2161 ggcaccgcgt gtacaagaac tacgatccgc gcgcgcgcat catccagaag gcctgccacg
     2221 acgtgcttgg cgagttgggc atcaaggacg acccgctgct cgacctggcc atggagctgg
     2281 agaagatcgc gctgaacgac aagtattttg tcgatcgcaa gctgtacccg aacgtcgatt
     2341 tctattcggg catcatcctg aaggcgatgg gcttccccac cagcatgttc accgcgctgt
     2401 tcgcggtgtc gcgcacggtc ggctgggtca gccagtggca ggagctgatg accgaccccg
     2461 agcagcgcat cggccggccg cgccagatct acaccggccc ggcacagcgg gattacgtgg
     2521 cggtcggcaa gcgcggctga cgggccgccc gtcctggccg gcgagggctg acc
//
LOCUS       DASSZH010000078        12049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_138497, whole genome shotgun sequence.
ACCESSION   DASSZH010000078 DASSZH010000000
VERSION     DASSZH010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 12049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12049
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138497"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VFN42_01555"
     CDS             complement(<1..677)
                     /locus_tag="VFN42_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HET8995331.1"
                     /translation="MRSARSCVICNELPVWPARPPCCHARATAFCPSSRNILLVPVMI
                     ASVAKASRRSVIKHAAVGAAACCLILAGCAPGSDLPSLPDASSGPYRIGVGEQLRIIT
                     FGDDRLTGQFKVNDQGEIALPLIGGVVAAGLTTSELQNRIAQRLKDHNVLLNPSVSVE
                     IQGYRPVYVLGEVAKPAQYPYEPGMTVLTAVTLAGGFTYRAQVQHASILRIIDGRPVE
                     GRVTRTT"
     gene            complement(643..1740)
                     /locus_tag="VFN42_01560"
     CDS             complement(643..1740)
                     /locus_tag="VFN42_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET8995332.1"
                     /translation="MAAVFLNGRFLSQPVTGVQRFSAEIVAAIDRLVASGEWPDTAVL
                     MPPPARAVLEQTQEPPERRYPYLRLRKIGRTHGHLWEQMELPGAARGRILVSLGNTAP
                     VLAGRRQVVVVHDAGIFDTPESYSLCFRTWYRTLLRGLVYAGAQVVTVSDFSRRRIAA
                     HVDLPVTRIAVVHEGAEHILRVAADPATLPRYGLRLRNYALVVGSRVAHKNLATLSEA
                     TALLRQRGMTIAVAGGADRDVFSGDVGAGSAERRLGRVSDAELRALYEGAACLLFPSR
                     YEGFGLPPVEAMACGCPVLAGRGGAVEEICTDHALYFDVTPHSVAGALACLLNEPALA
                     EDLRQRGRAHAARLNWTASARALGAIVRDLQ"
     gene            complement(1787..4075)
                     /locus_tag="VFN42_01565"
     CDS             complement(1787..4075)
                     /locus_tag="VFN42_01565"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HET8995333.1"
                     /translation="MAAARLEPLAVPFGRFTSTLRRHLWVVLLCPVLIVGIAFAILRQ
                     MPKQYTAEASILIEPQRTQVSDLQAISPDSGDVASLLRTQIDILRSPSLTLQVVHALH
                     LVQTPEFQPHGGLSAHLRRLLQRFGWHAAPLQAPPQQQMAERVAMMLSDKISFVNEAR
                     SSVLRVAVTTQNARLSADIANKIAAVYLDFKRQEKFEAMQRAHDWFQEQMGKLAGQLR
                     ADGLAVEQYRLQHRLDDQPPDDGTPGARPASINRQQLDAISNQIVQVARERALKEGQL
                     ADAEAVMRGQAVGSTLPAVLASPVIVDLLGQIATATGREAQLASQDGVGNPDLIAARA
                     QLRRLQGRLQGEMANICTSLRADVRAARMQEQLLRDQMEHLRQAVSGENAAQVPLQVL
                     QTRERATRSIYESFLVRATQLANVAGIQEPDASLVSSAQPPLGPSGPQVTRLVVVAGA
                     LSLVLGIGLAFAIERLCRGFTQPEQLEAVLGLPLLAMIPNVPRKALRCRGGRADMSVT
                     AALDNMRGQLRALGEARPRLVMVTSALPQDGKSVFAAEFARNAAAAGWRVMLVECDLY
                     RPSLAEHFRIAAAPGLCDILAGRLLGDLGKVVHEVAPRLHVIVGGRPDGDTQELLASN
                     RMTALLAAVRTRYDLVVLDTPPVLPVADTLVLVRHADATLLVVRWEKTARHAVQDGMR
                     RLRECRANLIGTVMTRVDPRRAAASTGRMSYSFSYNAYYATRLHPDRRIKMPVSLFGR
                     VLIHLLTGIFRIAGFRKPLPGS"
     gene            complement(4211..4999)
                     /locus_tag="VFN42_01570"
     CDS             complement(4211..4999)
                     /locus_tag="VFN42_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8995334.1"
                     /translation="MPLQLLDPELTGAGLSGEGLELSGEGTKSSGLDEPSPHTMKIGI
                     IDCYRFSQECLVKALENLDPEPTVYPFASVKGCIAEGRSDLDLIIYYSHVHAGSDIAP
                     IDAAAAVGQAFPNVPLVIMSDADDAQQLKMIRSTLQNGAHGFIPTRTTGIPIAIAAIR
                     LVGAGGIFAPMDAMLVNRPERAPALPDSVLQCHLTSRQMAVLMHIQRGKANKIIAHEL
                     GMSESTVKVHVRNIMRKLGATNRTQAVYKAQRLLEAATAARAVG"
     gene            complement(5536..5664)
                     /locus_tag="VFN42_01575"
     CDS             complement(5536..5664)
                     /locus_tag="VFN42_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995335.1"
                     /translation="MTFRWRADWIRIHQKAAGTVKKGRLQRSGACVPPAIGAVPSR"
     gene            complement(5791..8121)
                     /locus_tag="VFN42_01580"
     CDS             complement(5791..8121)
                     /locus_tag="VFN42_01580"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET8995336.1"
                     /translation="MPRAPRPSAIRNVSRRTLLAGGLALGGGLVLAMRLRPALGRYAG
                     KVATGTGGMPPGTISDPRVFVAVAPDGTVSIVAARAEMGAGSARTSLPMIVAEEMEAD
                     WSRVTVVQAPGDEQRYGSQDTDGSRSMRHFLQPMRECGAAARMMLEAAAARRWGIDPA
                     RVAARDHAVVDTASGRRLGFGELAAAAAVLPVPARDTLVFKDPQTYRYVGKGTVRIVD
                     LHDITVGLAAYGADHRLPGQRYAVIARPPVTGGRLVSFDATAALRVPGVEHVTEVPGW
                     QPPGTKFLPLGGVAVVARNTGAAILGREALRVVWDDGPNKDYESDAYRAALQGAVRVP
                     GQVVRSTGDVDEAMRSAAQVVSAEYYIPHLAHATMEPPAALVRVADGTCEAWAPVQSP
                     GGTRDDLARLLDLPAEKVTLHTQLLGGGFGRKSKCDYVLEAALLSKQLGVPVQVQWTR
                     EDDIRHGFYHAVAVERIEAALDADRKVVAWRHRSAAPSTATTFKAGPTHQAAFEASMG
                     LLDVPFDIPNIRCETGAAPAHTRIGWFRSVYNIPHGFAVQSFVAELAHATGRDAKDML
                     LELIGPPRLVDVRAQTEYGRFWQPMFWNYGESWDAYPIDTGRLRRVAEVAAEQAGWGR
                     RLPPGHGLGIAAHRSFVSYVATVVEVAVAEDGSITVPRVDTAIDCGFHVNPERIASQV
                     EGAAVMGLTLAKYGEITFKDGRVQQGNFDDYQMVRIDEAPRLTKVHIVRQPVPAHPGG
                     VGEPPLPPFAPALCNAIFAATGRRIRRLPIGDQLRG"
     gene            complement(8123..8581)
                     /locus_tag="VFN42_01585"
     CDS             complement(8123..8581)
                     /locus_tag="VFN42_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET8995337.1"
                     /translation="MNRLRLRVNGQERTTDADPVLPLLWYLRDELNLTGTKFGCGRAL
                     CGACTVHLDGQAVRSCSIRLADVRGRAVVTIEGLDPDGEHPVQKAWRAVSVPQCGFCQ
                     SGQIMQAASLLAANPKPSDAEIEAAMGGNICRCGCYQRIQAAIRLAAAEA"
     gene            complement(8594..9922)
                     /locus_tag="VFN42_01590"
     CDS             complement(8594..9922)
                     /locus_tag="VFN42_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995338.1"
                     /translation="MSAKFIFYVGTGLLAITGSAVATQTPAAATRAVPVDAFLDSLGV
                     DTHISQGYNYTNYIPALRYLGLRAIRDSTKRPDGLLAVHRATGVRADIGNAGDLPELL
                     RVGHILAAAGALLSFEGANEPNNFPITYHGKRGGSFKSWVAVAEFQRDLYASVKRDPV
                     LRSYPVFHVSEGGAEADNVGMQWLTIPDGAGTVMPAGTKYADYANPHNYVIGNCGRYL
                     DNDSWQAADPVLNKCWDGLYGEYGRTWAKRFPGYSDAALQTLPRVTTETGWDSVSDPG
                     GETVQGKVLTNTYLAQYARGWTYTFIYELGDGEGSSGHQGLFHADWTPKLAATYIHNL
                     TTILATAQATPAAAAMGEAANTLAYTIPDKPATVHDLLMQKPDGSFALAVWGEQVTGV
                     NDVTLALGSRHAQVRVYDITSGTAPVRTLADVDRVPLTLSDHALIVEITP"
     gene            10200..11552
                     /locus_tag="VFN42_01595"
     CDS             10200..11552
                     /locus_tag="VFN42_01595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8995339.1"
                     /translation="MLQTFRMSLAENQAPAGLPMAMLRDDPEPIHDDTDLMSARRGML
                     DQVAAQTRWQSGVAAAVLSTRMPPAAHRRPTPRIAIVHEWLDSYSGSERVVEQLLQCF
                     PEADLFAVVDFLKEGQRGFLDGRAVRTTFIQNLPFARHMFRCYLGLMPIAIEQLDLSG
                     YDLVISSNHAVAKGVITGPDQLHISYVHSPMRYAWDMQHQYLGRRLGPKQLYARWLFG
                     RLREWDAASAHHVDHFVANSHYIARRIRKVYRREAVVIHPPVDIGRFSMRASKDDFYL
                     LACRFVPYKRAEIVVESFARQPRRRLLVVGSGSEEARVRAAARGAGNIRFLGTVSQDR
                     LIDLMQRARAFVFAGEEDFGITFVEAQACGTPLIAFDRGGVTDILVPPEAESPTGVLF
                     DRQDPEAVTAAVIRFEALGTRITAEACWRNATRFGQERFRAEIGGYVERALGAFPEFH
                     "
     gene            11777..12031
                     /locus_tag="VFN42_01600"
     CDS             11777..12031
                     /locus_tag="VFN42_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET8995340.1"
                     /translation="MTDNEILGALNAIFRHVLDDPSIALTPSTTADDVPGWDSMTQIT
                     LAVEIEHALGVKFKTSEMEEMRNIGDLVALVHTHLTAVAG"
BASE COUNT         1926 a         4086 c         4046 g         1991 t
ORIGIN      
        1 ttcgtggtgc gcgtcacgcg gccctcgacc ggtcggccgt cgatgatgcg caggatggag
       61 gcatgctgca cctgggcacg ataggtgaat ccgccggcga gggtcacggc ggtcagcacg
      121 gtcatgcccg gctcgtatgg atactgcgca ggcttggcga cctcgcccag gacatagacc
      181 ggccggtagc cctggatctc caccgaaacc gaaggattaa gcagcacgtt gtggtccttg
      241 aggcgctgcg cgatccggtt ctgcagttcc gacgtggtca gccccgccgc cacgacgccg
      301 ccgatcagcg gcagcgcgat ctcgccctgg tcgttgacct tgaactggcc ggtgagacgg
      361 tcgtcgccga aggtgatgat ccgcagctgc tcgccaacgc cgatccggta cggcccggac
      421 gatgcgtccg gcagcgacgg caggtccgac cccggcgcac agccggccag gatcaggcag
      481 cacgccgccg cgccgacggc ggcgtgcttg atgacggaac gacgactggc cttggcaaca
      541 gaagcaatca tgaccggcac cagcaggatg ttgcgggaag atggacaaaa ggcggtcgcc
      601 cgcgcgtggc aacagggagg tcgcgccggc cagacaggca gttcattgca aatcacgcac
      661 gatcgcgccg agcgcacggg ccgaggcggt ccagttcagg cgtgccgcgt gcgcccgtcc
      721 gcgctgccgc aggtcctcgg caagcgccgg ttcgttcagc aggcacgcca gcgcgccggc
      781 gaccgagtgt ggagtgacgt cgaaatacag cgcgtgatcc gtgcagatct cctccaccgc
      841 tccgccgcgg ccggcaagca cggggcagcc gcaggccatt gcctcgaccg gcggcaggcc
      901 gaagccttcg tagcgtgacg ggaacagcag gcaggccgcg ccctcgtaaa gcgcccgcag
      961 ctcggcatcg ctgacgcggc cgaggcggcg ttcggcggag cctgcgccga catcgccact
     1021 gaacacgtca cggtcggcgc cacccgcgac ggcgattgtc atgccacgct gccgcaacag
     1081 ggcggtcgcc tcggagaggg tcgcgaggtt cttgtgtgcg acacggctgc cgaccaccag
     1141 ggcatagttc cgcaggcgca ggccgtagcg cggcaaggtt gccggatcgg cggcgacgcg
     1201 caggatatgc tcggcacctt catggacgac ggcgatgcgc gtgaccggga ggtctacatg
     1261 cgcagcgatg cgccggcgcg agaagtccga caccgtgacc acctgcgcgc cggcgtacac
     1321 cagcccgcgc aacagcgtcc tgtaccacgt gcggaagcac agggaatagg attcgggtgt
     1381 gtcgaagatg ccggcgtcat ggaccaccac cacctgccgc cgcccagcca gcaccggcgc
     1441 ggtgttgcca aggctgacca ggatcctgcc gcgcgctgcg cccggtagct ccatctgctc
     1501 ccacagatgg ccgtgtgtgc ggccgatttt ccgcaggcgc aggtacgggt agcgccgttc
     1561 cggcggctcc tgcgtctgct ccagcacggc gcgcgcgggg gggggcatga ggacggcggt
     1621 gtccggccat tcgccgcttg ccaccagccg atcgattgcg gcgacgatct cggccgagaa
     1681 gcgctgtacg ccagtcaccg gctggctcag gaatcgcccg ttcaggaaca ccgctgccat
     1741 cagacgtcca tttcgttgcc gctgcccgtg cggggtgttg cctgcgtcag cttcccggca
     1801 gcggcttgcg gaagcccgcg atgcggaaaa tgcccgtcag caggtggatg agcactctgc
     1861 cgaaaaggct gacgggcatc ttgatgcggc ggtcggggtg gaggcgggtg gcgtagtagg
     1921 cattgtagct gaagctgtag gacatgcggc cggtcgatgc ggcggcacgc cgcgggtcga
     1981 cgcgggtcat gacggtgccg atcaggttcg cccggcattc gcgcagccgc cgcataccgt
     2041 cctgcaccgc gtgccgggcc gtcttctccc agcgcaccac cagcagtgtg gcgtcggcat
     2101 ggcggaccag caccagcgta tcggccaccg gcagcaccgg cggcgtgtcc agcaccacca
     2161 ggtcgtagcg cgtgcgcacc gcggcgagca gcgccgtcat gcggttcgag gccaacagtt
     2221 cctgcgtgtc tccgtccggc cttccgccga cgatgacgtg cagccgcggc gccacctcat
     2281 gcaccacctt gcccaaatcg ccgagcaggc gtccggccag gatgtcgcac agccccggcg
     2341 cagcggcgat tcggaaatgc tcggccagcg aaggacgata caggtcgcat tcgaccagca
     2401 tcacccgcca gcccgccgcc gcggcgttgc gcgcaaactc ggccgcgaac accgacttgc
     2461 cgtcctgcgg cagcgccgac gtgaccatga ccagccgggg ccgcgcctcg ccaagcgcgc
     2521 gcagctgccc gcgcatgttg tcgagcgccg cggtgacgga catgtcggcg cgcccgccgc
     2581 gacagcgcag ggccttgcgc ggcacgttcg gaatcatcgc caacagcggg agtccgagca
     2641 cggcctccag ctgctcgggc tgggtgaagc ccctgcacag ccgctcgatt gcgaaggcca
     2701 gtccgatccc gagcaccagc gacagcgcgc ccgccaccac caccagccgg gtgacttgcg
     2761 ggcccgatgg gccgagcggc ggctgggcgc tggaaaccag cgaggcatcc ggctcctgga
     2821 tgccggcgac gttggcaagc tgcgtcgcgc gcaccaggaa gctctcgtag atgctgcggg
     2881 tcgcacgttc gcgggtctgc agcacctgca gcggcacctg ggcggcgttc tccccactca
     2941 ccgcctggcg cagatgctcc atctggtcgc gcagcaactg ctcctgcatg cgggcggcgc
     3001 gcacgtcggc acgcaggctg gtgcagatat tggccatctc gccttgcagc cgcccctgta
     3061 gccggcgcag ctgcgcgcgt gccgcgatca ggtccgggtt gcccacccca tcctggctgg
     3121 ccagttgcgc ctcccgcccg gtggcggtgg cgatctggcc gagcagatcg acgatcaccg
     3181 gcgaggcgag caccgccggc agcgtgctgc ccaccgcctg gccgcgcatg accgcctcgg
     3241 cgtcggcgag ttgcccttcc ttgagcgccc gctcgcgcgc cacctgcacg atctggttgc
     3301 tgatcgcgtc gagctgctgg cggttgatgc tggcgggacg cgcgccgggc gtgccgtcgt
     3361 ctggcggctg gtcgtccagc cgatgctgca ggcggtactg ctcgacggca agcccgtcgg
     3421 cgcgcagctg gccggcgagc ttgcccatct gctcctggaa ccagtcatgg gcgcgctgca
     3481 tcgcctcgaa tttctcctgc cgcttgaagt cgaggtacac ggcagcgatc ttgttggcga
     3541 tatcggcgct gagcctggcg ttttgcgtgg tgactgcgac gcgcagcacg ctggaacggg
     3601 cttcgtttac aaagctgatc ttgtcgctga gcatcatcgc cacgcgctcg gccatctgct
     3661 gctgcggcgg cgcctggaga ggcgccgcat gccagccgaa ccgttgcaac agcctgcgaa
     3721 ggtgggccga cagcccgcca tggggctgga actccggcgt ctgcaccagg tgcagtgcat
     3781 gcaccacctg caacgtcagc gaaggcgagc gcaggatatc gatctgtgtg cgcagcaggc
     3841 tcgcgacatc gcccgaatcg ggtgagatcg cctgcaagtc gctgacctga gtgcgctgcg
     3901 gctcgatcag gatggaggcc tcggcggtgt attgcttggg catctgccgc aatatggcga
     3961 aggcgatgcc gacgatcagc accggacaca gcagcaccac ccagagatgc cggcgcagcg
     4021 tcgaggtgaa ccgcccgaac ggaaccgcca gcggctccag cctcgccgcc gccatctccg
     4081 gtgctatagg gagggccgag gcacctgccg gctcggaaga gtgtggcagc tggtccaaca
     4141 tgatgttgct caacccatct ggccgccgcc cgccggggcg ggcggcgggt caccgccgtt
     4201 caccctggat tcagcctacc gcgcgggccg ccgtcgcggc ttcaagcagc cgttgggcct
     4261 tgtagaccgc ctgcgtgcgg ttggtggcgc cgagcttgcg catgatattg cggacgtgga
     4321 ccttgaccgt gctttcgctc atgccgagct cgtgggcgat gatcttgttg gccttgccgc
     4381 gctgtatgtg catcagcacc gccatctgcc gcgacgtgag gtgacattgc agcacgctat
     4441 cgggcagcgc gggggcacgt tccggccggt tgaccagcat cgcgtccatt ggcgcgaaga
     4501 tgccgcccgc accgaccagg cgaatcgccg cgatggcaat cgggatgccc gtcgtgcggg
     4561 tcgggatgaa accatgcgcg ccgttctgca gcgtgctgcg gatcatcttg agctgctggg
     4621 catcgtcagc atcggacatg atgaccagag ggacattggg aaacgcttgg ccgactgcag
     4681 cggcggcatc gatgggcgcg atgtcggacc cggcatggac gtgcgagtaa tagatgatca
     4741 gatcgaggtc gctgcgtcct tcggcgatgc agcccttgac gctggcgaag gggtagacgg
     4801 tcggttccgg atccagattt tccagcgcct tgacaaggca ttcctggctg aagcgatagc
     4861 agtcgatgat gccgatcttc atcgtatgcg gcgacggctc gtccagcccg gacgacttcg
     4921 ttccttcacc ggataattcc aatccttctc cggacagtcc agcgccggta agttcgggat
     4981 cgagaagttg cagtggcatg ctgcactccg cgtctaagtt ctgtatggct ttcaatgaat
     5041 ttgctccccc atacgatcca gtggaaacgt tgcgatcgct tttgtattct tgccgatgaa
     5101 gcggacgcgt ggaaaccgat ttccaagacg tacacaaaag ttttattgaa tataggcggg
     5161 tttttttcaa aagccgtaga gcggacttcg atcaatacgg tgatcgttca tccgaaaggc
     5221 ggagattttc aatcatcatg gggatttctt atatatatag tggcatagta ttgttattat
     5281 cagaattttt aataactatc tctagttgag atcggcgaat ctccggctgt cgtccatgtc
     5341 tggaggcagg cacgagaaac gacatggatg ggaggatttt gcggcggtct gcgaccagga
     5401 ttggccgctg cactccgcgc cacgggtgtg ggcgtggcca tctgccagcc gaaatggtac
     5461 ggctgccgag tacgctgcga cggcttctgc gcgcaggcgc ggcatggtgc gacctgaagg
     5521 cgaacgagcg taatgtcagc ggctcggcac tgcgccgatc gcgggtggca cgcaggcccc
     5581 gctgcgctgc agccgtccct ttttgaccgt gccggcggct ttctggtgga tgcgaatcca
     5641 gtcggcgcgc cagcggaagg tcatgccgga tgcgatggga taaagccctg cgcggcccca
     5701 tggcccgaac tcagcgggca gagggcgtca ttgcaccccg ttctgatgcc gccacaggat
     5761 cgcgctgatc gttcgccgcg gcggcactgc ttatcccctg agttgatcgc cgatcggcag
     5821 gcggcggatg cgtcggccgg tcgcggcgaa gatcgcgttg cacagcgccg gcgcgaacgg
     5881 tggcagcggc ggctcgccga cgccgcccgg atgggccggg acgggctgcc gcacgatgtg
     5941 gaccttggta agccgaggcg cctcgtcgat gcgcaccatc tggtagtcgt cgaaattgcc
     6001 ctgctgcacc cggccgtcct tgaacgtgat ctccccatac ttggcgagcg tcaggcccat
     6061 gactgccgcg ccctccacct gcgaggcaat gcgctccgga ttgacgtgaa agccgcagtc
     6121 gatcgcggta tcgacacgtg ggacggtgat gctgccgtcc tccgccaccg caacctccac
     6181 cacggtcgcc acgtagctga cgaagctgcg gtgcgcggct atgccaagcc cgtggccagg
     6241 gggcagcctt cgtccccagc ccgcctgctc cgccgccacc tccgcaaccc ggcgcagccg
     6301 gccggtgtcg atcgggtagg cgtcccacga ttcgccgtaa ttccagaaca tcggctgcca
     6361 gaaccgaccg tattcggtct gcgcacgcac atcgacgagg cgcggcgggc cgatcagctc
     6421 cagcaacatg tccttcgcgt cgcgcccggt cgcgtgcgca agctcggcca cgaaactctg
     6481 caccgcgaag ccgtgcggga tgttgtagac ggatcggaac cagccgatgc gcgtgtgcgc
     6541 cggcgcggca ccggtctcgc agcgtatgtt gggtatgtcg aacggcacgt ccagcagccc
     6601 catgctcgcc tcgaaggccg cctggtgggt tgggccggcc ttgaacgtgg tcgcggtgga
     6661 aggtgccgcg ctgcggtggc gccaggcgac gaccttcctg tcggcatcga gcgccgcctc
     6721 gatgcgctcg accgcgacgg catgatagaa gccatggcgg atgtcgtcct cgcgcgtcca
     6781 ctgcacctgc accggcacgc ccagttgctt cgacagcaag gccgcctcaa gcacgtagtc
     6841 gcatttcgac ttgcgaccga agccgccgcc gagcagctgc gtgtgcagcg tcaccttctc
     6901 ggccggcagg tcgagcagcc gggcaaggtc gtcgcgcgtg ccgcccgggc tctgcaccgg
     6961 cgcccaggcc tcgcacgtgc cgtccgccac acgcacgagg gccgcgggcg gctccatcgt
     7021 cgcgtgcgct aggtggggga tgtagtattc ggcgctgacc acctgcgccg ccgaccgcat
     7081 cgcctcatcc acgtcgcccg tgctgcgcac cacctggccg ggcacgcgca ccgccccctg
     7141 cagcgcggcg cgataggcat cggactcgta gtccttgttc ggcccgtcgt cccagaccac
     7201 gcgcagcgcc tcccgcccca ggatcgcggc accggtattg cgcgcgacca cggcgacgcc
     7261 acccaacggc aggaactttg tccccggcgg ctgccagccc ggcacttccg tcacatgctc
     7321 gacgccggga acgcgcagcg ccgccgtggc gtcaaaggag acgagcctgc cgcctgtcac
     7381 cggcggccgc gcgatcaccg cgtagcgctg cccgggcagc cggtgatcgg cgccataggc
     7441 cgccaggccg accgtgatgt cgtgcagatc gacgatccgc accgtaccct tgccgacata
     7501 gcggtaggtc tgtggatcct tgaagacgag cgtgtcgcgc gcgggcaccg gcagcactgc
     7561 cgcggcggcg gccagctcgc cgaatccgag ccgccggccg gaggcggtgt ccacgaccgc
     7621 gtggtcgcgc gccgcgaccc gcgcgggatc gatgccccat cgccgcgccg ccgccgcttc
     7681 cagcatcatc cgcgcggcgg cgccgcattc gcgcatcggc tgcagaaagt ggcgcatgct
     7741 gcgggagcca tcggtgtcct ggctgccgta gcgctgctcg tcgcccggcg cctgcaccac
     7801 ggtcacacgc gaccagtcgg cctccatctc ctcggcgaca atcatcggca ggctggtgcg
     7861 cgccgatccc gcgcccatct cggcccgcgc ggcgacgatg ctgacggtgc cgtccggcgc
     7921 gaccgcgacg aacacgcgcg ggtcgctgat cgtaccgggc ggcatgccgc cggtgccggt
     7981 tgccaccttt ccggcgtacc ggccaagggc cggccgcagc cgcatcgcca ggaccaggcc
     8041 gccgccgagc gccagcccgc ccgccaacag ggtacggcgg ctgacattgc ggatcgcgga
     8101 aggccggggc gcgcgcggca tgtcacgcct ccgctgcggc caggcggatc gctgcctgaa
     8161 tccgctggta gcagccgcag cggcagatgt tgccgcccat cgccgcctcg atttcggcat
     8221 cgctgggctt cgggttggcg gcgagcaggc tggcggcctg catgatctgc ccgctctggc
     8281 agaagccgca ttgcggcacg ctgaccgcgc gccaggcttt ctgcaccgga tgctcgccgt
     8341 ccggatcaag cccctcgatc gtgacgaccg cccggccgcg cacgtccgca agccggatgc
     8401 tgcacgagcg gaccgcctgc ccatcgagat gcaccgtgca ggcaccgcac agcgcccggc
     8461 cacagccgaa cttggtcccg gtcaggttca gttcgtcgcg cagataccac agcagaggca
     8521 gcaccggatc ggcatccgtc gtgcgttcct gcccgttgac gcgcagcctc agcctgttca
     8581 tcggtcacct ccgctacggc gtgatttcga cgatcagcgc gtggtcgctc agcgtcagag
     8641 gcacgcggtc aacatcggca agcgtgcgca ccggcgccgt gccgctggtg atgtcgtaga
     8701 cccgcacctg cgcatggcgg ctgccgagag cgagggtcac gtcattcacg cccgtgacct
     8761 gctcgcccca caccgccagg gcgaagctgc catccggctt ctgcatcagc agatcatgca
     8821 cggtcgccgg cttgtccggg atcgtgtagg cgagcgtgtt cgccgcctcg cccatcgccg
     8881 ctgccgccgg cgttgcctgc gcggtggcaa ggatcgtggt gagattgtgg atgtacgtgg
     8941 cggcgagctt cggggtccag tccgcgtgga acaggccctg gtggccgctg ctgccctccc
     9001 cgtcgccaag ttcgtagatg aaggtatagg tccagccgcg ggcgtactgg gcaagatagg
     9061 tgttggtcag taccttcccc tgcaccgtct cgccgccagg gtcggagacc gagtcccacc
     9121 ctgtctcggt ggtcacgcgc ggcagcgtct gcagtgcggc gtcggaatag ccgggaaagc
     9181 gcttcgccca agtgcggccg tattcgccat acagcccgtc ccagcacttg ttcagcaccg
     9241 ggtcggcggc ctgccaggag tcattgtcca gataacggcc gcaattgccg atgacgtagt
     9301 tatgtggatt ggcatagtcg gcatatttcg tgccggctgg catcaccgtc ccggcgccgt
     9361 cgggaatggt caaccattgc atcccgacat tgtcggcttc cgccccgcct tccgagacgt
     9421 ggaacaccgg ataggaccgc agcaccggat cgcgcttgac acttgcatag aggtcccgtt
     9481 ggaactcggc tacggcgacc caggacttga agctgccgcc gcgtttgcca tgataggtga
     9541 tcgggaagtt gttcggctcg ttggcgccct cgaagctgag cagcgcgccg gcggcagcca
     9601 ggatgtggcc cacgcgcagc agttcgggca ggtcgcccgc attgccgatg tcggcgcgca
     9661 cgccggtcgc gcgatggacc gccagcaaac cgtcgggccg cttcgtgctg tcgcggatcg
     9721 cgcgcagccc caggtagcgc agcgccggaa tataattcgt gtagttgtag ccctgcgaga
     9781 tatgcgtgtc gacgccgagc gaatcgagaa acgcatcgac cgggaccgcc cttgtcgccg
     9841 cagccggtgt ttgcgtggcg accgccgatc cggtgatggc cagcaggccg gtgccgacgt
     9901 aaaagatgaa tttggctgac aatgtcgcgc ccccctgagt tgagtgcgtc ccctgtgtcg
     9961 atgtgcgggc ggcctgcccc gaaggcgcgc cggtactgcc ggccgctcgt actccgctgc
    10021 ggcgaccgtg cgcgacccgg gggaggcgcg caaccgcgtc aagattggtc gtcgcggcgc
    10081 cgctacgcac attggctgaa cgctggtcgc atgtccccct caaagaggcg gcttactggc
    10141 cggccggacc gcgggcatta tttgtgtggc gcgaccgtcc tttcgccacc gatgcttcgt
    10201 tgctgcagac gttcaggatg tcccttgccg aaaaccaagc acctgcgggg ttgccgatgg
    10261 ccatgctgcg cgatgatccg gaacccatcc acgacgatac cgatctgatg tccgcccgcc
    10321 gcggcatgct tgaccaagtg gcagcgcaga cgcgctggca gagcggcgtg gccgccgctg
    10381 tgctgtccac ccggatgccc cctgcggcgc accgccgacc cacgccgcgc atcgccatcg
    10441 tgcatgagtg gcttgacagc tacagcgggt cggagcgggt ggtggagcag ctcctgcaat
    10501 gctttcccga agccgacctg ttcgccgtgg tggatttctt gaaggagggg cagcgcggct
    10561 tcctcgacgg ccgcgcggtg cggaccacct tcatccagaa cctgccattc gcccggcaca
    10621 tgttccgttg ctatctgggc ttgatgccga tcgcgatcga acaactcgac ctgagcggct
    10681 acgatctcgt tatctcaagc aaccatgccg tcgccaaggg cgtcataacc ggcccggacc
    10741 agctgcacat cagctacgtc cactcgccga tgcgctacgc ctgggacatg cagcaccagt
    10801 atctgggccg ccgcctcggt cccaagcagc tctacgcgcg ctggctgttc ggccggctgc
    10861 gcgaatggga tgccgcctcc gcccatcatg tcgatcactt cgtggccaat tcccactaca
    10921 ttgcgcggcg gatccgcaag gtctaccggc gggaagcggt ggtcatccac ccgccggtcg
    10981 acatcggccg cttttcgatg cgagccagca aggatgattt ctacctgctg gcctgccgct
    11041 tcgtgcccta caagcgggcc gagatcgtgg tggagagctt cgcgcgccaa ccgcgtcgcc
    11101 gtctcctcgt ggtcggcagc gggtcggagg aagcgcgcgt gcgggcagcg gcgcgcggtg
    11161 cgggcaatat ccgcttcctt gggacggtat cgcaggatcg gctcatcgac ctgatgcaac
    11221 gcgcgcgggc gttcgtgttt gcgggcgagg aggatttcgg catcaccttc gtcgaggcgc
    11281 aggcctgcgg cacgccgctc atcgccttcg accgcggtgg cgtcacggac atccttgtgc
    11341 cgcccgaggc cgaaagcccg accggggtgt tgttcgaccg ccaggacccg gaggccgtca
    11401 cggcggcggt cataaggttc gaggcactcg gaacccggat taccgccgag gcgtgctggc
    11461 gcaacgccac ccgcttcggg caggagcggt tccgcgccga gatcggcgga tacgtcgagc
    11521 gggcgctcgg cgccttcccg gaattccatt gacgggaccc gtttcactgc ttctccggcg
    11581 cgctttgcgc cgcgaccggc tgatcgcggc ccgcagggac acggagacag gtgaatgccg
    11641 gccactagcc gaatggacga actgacagcg aaggtcgaac cccgcataaa cctatcccgt
    11701 ggtcgcccgt gctgcgcagc cgcatgctcc ggcgcgtcgc gacgcaaggc cacatgacat
    11761 cccgaaaggc ccggcggtga cagacaacga aatccttggt gccctgaatg caatcttccg
    11821 gcatgtgctg gatgatccat ccatcgcgct gacgccgtcc actacggccg acgacgttcc
    11881 cggatgggac tcgatgacgc agatcacgct cgcggttgag atcgaacatg cgctaggcgt
    11941 gaagttcaag acgtcggaga tggaggagat gcgcaacatc ggggatctgg tggcgctggt
    12001 gcacacgcat ctcacagccg tcgccggata gcagcggccg gccacgcgg
//
LOCUS       DASSZH010000079         2408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1246598, whole genome shotgun sequence.
ACCESSION   DASSZH010000079 DASSZH010000000
VERSION     DASSZH010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246598"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2007
                     /gene="pta"
                     /locus_tag="VFN42_01605"
     CDS             <1..2007
                     /gene="pta"
                     /locus_tag="VFN42_01605"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HET8995341.1"
                     /translation="KAGFAKPIGQPEARGGIDLSTYFARSLLHVDVPDSIPFATAEER
                     VRTGQIDALMEDVVSALDAARTGCDVMVVEGLIPDADLQVAAQLNAAMACSLSAALVP
                     VLSGTACRTQGPRNCIDLALRQYSAGGAELPVMGGVLVNKLADPAYAASLRSTLADAG
                     TPLLGAVPYSAELEALRLLDVVQALDLQVEHPGNMSRSRVREFVVAGRGVEGVIDRLR
                     PGALVLTAGERSDIALASGLAFLQGTPLAGLLLTCGTQLTPQVALLLHSAALAGLPIL
                     SSSDDTFATATRVAGLSRHVRADDSERMERTIAFVAEHTDTAPLRERLGRPGRLRMPP
                     PAFRHRLVQAARAANKRIVLPEGEEPRTLQAAAICQRKGIARCVLLGDPARVRQAALA
                     QGVELPDDMEIVDPAHVRARYVAPMVELRRAKALTAFQAEQQLEDNVVLGTMMLALDE
                     VDGLVSGAVHTTANTVRPALQLIRTAPGVSIVSSGFFMLLPEEVLVYGDCAVNPDPTA
                     EELADIAIQSADSARAFGIEPRVAMISYSTGNSGTGADVEKVCRALALARAQRPDLTI
                     DGPMQYDAASVESVGRQKAPGSPVAGRANVFVFPDLNTGNTTYKAVQRSAHVISVGPM
                     LQGLRKPVNDLSRGALVDDIVYTIALTAIQATQPQAATEQLAAA"
BASE COUNT          370 a          844 c          833 g          361 t
ORIGIN      
        1 aaggcgggat ttgccaagcc gatcggccag ccggaagcac gcggcggcat cgacctgtcc
       61 acctatttcg cccgctcgct gctgcatgtc gatgtgccgg actccattcc cttcgccacc
      121 gccgaggagc gcgtgcgcac cgggcagatc gacgcgctga tggaagacgt cgtctccgca
      181 ctcgacgccg cccggaccgg ctgcgacgtg atggtggtgg aagggctgat ccccgacgcg
      241 gatctgcagg tcgcggcgca gctcaatgcg gccatggcct gcagcctgtc cgccgcgctg
      301 gtcccggtgc tgtccggcac cgcctgccgc acgcaggggc cgcgcaactg catcgacctc
      361 gccttgcggc aatacagcgc cggcggcgcc gaattgccgg tgatgggagg cgtgctggtc
      421 aacaagctgg ccgaccccgc ttatgccgca tcgctgcggt ccacgctggc cgatgccggc
      481 acgccgctgc tcggcgcggt gccctattcc gccgaactgg aagcgttgcg cctgctggac
      541 gtggtgcagg ccctggacct gcaggtggag caccccggca atatgtcgcg ttcccgcgtg
      601 cgcgagttcg tcgtggcggg acgcggcgtg gaaggcgtga tcgaccgcct gcgccccggc
      661 gcgctggtgc tgaccgcggg cgaacgcagc gacatcgcgc tggcgtccgg cctggcgttc
      721 ctgcaaggca cgccgctcgc cggcctgctg ctgacctgcg gcacccagct caccccgcag
      781 gtggcgctgc tgctgcactc cgccgcgctg gccggcctgc cgatcctgag ttcgtccgac
      841 gacacgtttg ccaccgccac acgcgtggcc ggcctgtcgc gccacgtgcg cgccgacgac
      901 agcgagcgca tggagcgcac catcgccttt gtcgccgaac acaccgacac ggcaccgctg
      961 cgtgagcgcc tgggccggcc cggccgcctg cgcatgccgc cgccggcgtt ccggcaccgc
     1021 ctggtgcagg ccgcgcgcgc cgccaacaag cgcatcgtgc tgccggaagg cgaggagccg
     1081 cgcaccttgc aagccgccgc gatctgccag cgcaagggca ttgcccgctg cgtgttgcta
     1141 ggcgatccgg cgcgcgtgcg tcaggccgcg ctggcacagg gagtggaatt gccggacgac
     1201 atggagatcg tcgatcccgc ccacgtgcgg gcgcgctatg tcgcgcccat ggtggaactg
     1261 cgccgcgcca aggcgctgac cgcgttccag gcggaacagc aactggaaga caacgtggtg
     1321 ctgggcacca tgatgctggc gctggacgag gtggacggcc tggtgtcggg cgcggtgcac
     1381 accaccgcca acacggtgcg cccggccttg caactgatcc gcaccgcgcc gggcgtttcc
     1441 atcgtctcca gcgggttctt catgctgctg ccggaggaag tgctggtgta tggcgattgc
     1501 gcggtcaatc ccgatcccac cgcggaagaa ctggccgaca tcgccatcca gtcggcagac
     1561 tcggcgcgtg ccttcggcat cgagccgcgc gtggcgatga tttcgtattc caccggcaat
     1621 tccggcaccg gcgccgacgt ggagaaagtg tgccgcgcgc tggccctggc gcgggcgcag
     1681 cgccccgatc tgaccatcga cgggccgatg cagtacgatg cggccagcgt ggaaagcgtc
     1741 gggcggcaga aggcgccggg cagtccggtg gcggggcggg cgaacgtgtt cgtgtttccc
     1801 gatctgaaca ccggcaatac cacctacaag gcggtgcagc gctcggccca tgtcatcagc
     1861 gtggggccga tgttgcaggg gctgcgcaag ccggtgaacg atttgtcgcg cggcgcattg
     1921 gtggacgaca tcgtctatac catcgcgctc accgcgatcc aggcgaccca gccgcaagcc
     1981 gcgacggagc aactggcggc ggcgtagccg cctccgttac cgtaaataga aatgctaacg
     2041 taatccggcg cgtacgtgtc tcgtcccgtc atggccgggc ttgacccggc catctacccg
     2101 gcaacgatcg ggtccggatg tgacggaaca cgccttgtcg ggggcggatg gccgggtcaa
     2161 gcccggccat gacacgggta ggtcaccact tcgttccgcc gtgtggtacg cgggggcgct
     2221 tcagcggcta catcgtcatg gcgggcgcag gcccgccatc cacgactggc gggcggagac
     2281 ccgaagccgc aaactgccat gcgcggagtc tcccccagcc agccgtggat ggcgggcctg
     2341 cgcccgccac gacgcggcac tcacgcctga accaaggacc caccgagcat gcagaacacc
     2401 gaagagat
//
LOCUS       DASSZH010000080         2550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_922469, whole genome shotgun sequence.
ACCESSION   DASSZH010000080 DASSZH010000000
VERSION     DASSZH010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922469"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VFN42_01610"
     CDS             <1..449
                     /locus_tag="VFN42_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET8995342.1"
                     /translation="QQSDLGRMLDPIADKLLVAAALMMLVGEGRVSNWGLYPAIVIML
                     REILVSGLREYLAGIRIGLPVTRLAKWKTTVQMTALGTLLAGDSAAGLLHIGFIPVSG
                     IGEVMLWIAAVLTLITGWDYLTAGLRHATTGPAQGNARSGVAAKQP"
     gene            446..949
                     /gene="mobB"
                     /locus_tag="VFN42_01615"
     CDS             446..949
                     /gene="mobB"
                     /locus_tag="VFN42_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HET8995343.1"
                     /translation="MKVLGIVGWSGSGKTTLLEAVLPLLRARRLTVSTVKHAHHGFDM
                     DRPGKDSFRHREAGAQEVLVATAVRWALLHEVSGPEPTLPELLERLAPVDLVLVEGFK
                     THPFAKLEVHRPALGKPPIWRQETGVVAVASDAALDVEGRVLLPLNDPAAVAAWIADF
                     AGSPAAA"
     gene            1081..1986
                     /locus_tag="VFN42_01620"
     CDS             1081..1986
                     /locus_tag="VFN42_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8995344.1"
                     /translation="MSNHPAISLVAVPGRRRQTIELAQEIERRGFAGIYSPSMFGNMS
                     LCEALAWNTRSIPFGTAIAPIYARTVGDFAQSSAFMHEISGGRFRLGIGVAHGPSHVR
                     MGVTPGKPLGDTRNFVERFRAQEGLGELPPIIIAALRKKMVALAGEISQGVVFANASL
                     SHMAESLAALPAAKRSDPAFFIGNMLPTCISDDVEAAKAVCRRSLTNYAFLPNYRNYW
                     KEAGYVEEMAAIERAIDEGRRDDVPRLFSDRWLADNTLFGPAAKVREGVEAWQAAGVH
                     TPVIVPSSASGNQLTALHEIFAAFG"
     gene            2020..>2550
                     /locus_tag="VFN42_01625"
     CDS             2020..>2550
                     /locus_tag="VFN42_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET8995345.1"
                     /translation="MLNALPDSIRAQWPYQPRYARVNGWRMHYIDEGSGDPVVLLHGN
                     PTWGFLYRDFVAPLTAAGHRVIIPDMIGFGLSEKPAREQDQSLDGHIANLAGLLRQLD
                     LHRITLVCHDWGGPTGLGFAFSNMARMRALVVMSTWGWPLPPAEFHTRIFPWRMMHAP
                     LLAPYLLGRHNALAARG"
BASE COUNT          377 a          876 c          894 g          403 t
ORIGIN      
        1 gccagcagtc cgacctcggc cgtatgctcg acccgatcgc cgacaagctg ctggtcgccg
       61 ccgcgctgat gatgctggtg ggcgaggggc gggtatccaa ctggggcctc taccccgcga
      121 tcgtgatcat gttgcgggaa atcctggtca gcggcctgcg cgaatacctg gccggcatcc
      181 gcatcggcct gccggtgacc cggctggcca agtggaagac cacggtccag atgaccgcgc
      241 tgggcacgct gctggccggc gacagtgccg ccgggctgct gcatatcggc ttcatcccgg
      301 tctccggcat cggcgaggtg atgctgtgga ttgccgctgt gctgaccctg atcaccggct
      361 gggactacct gaccgccggc ctgcgccacg ctacgaccgg cccggcgcag ggcaacgccc
      421 gatccggggt ggcggcaaag cagccgtgaa ggtcctgggt atcgtcggct ggtccggcag
      481 cggcaagacc acgctgctcg aagcggtgct gccgctgctg cgcgcgcgcc gcctgaccgt
      541 ctccaccgtc aagcacgcgc atcatggctt cgacatggac cggcccggca aggacagttt
      601 ccgccaccgg gaggccggcg cgcaggaggt gctggtggcg accgccgtcc gctgggcatt
      661 gctgcatgag gtcagcgggc cggaacccac gctgccggaa ctgctggagc ggctggcccc
      721 ggtcgatctg gtgctggtgg agggcttcaa gacccatccc ttcgccaagc tggaagtgca
      781 ccgcccggcg ctgggcaaac cgccgatctg gcggcaggag accggtgtgg tcgcggtcgc
      841 ctccgacgcg gcgctggacg tggaaggccg ggtgctgctg ccgctgaacg atcccgcggc
      901 ggtcgcggcg tggatcgcgg acttcgccgg ctcgcctgcc gcggcctagg cgccgccggc
      961 gtgcgcggcc tgtaacggcg tgcgcagatt cttccccagg gcgatcgcgg cgttgcctcg
     1021 ccggattgcg agtgcggccg ggatgggtag catgccggcg aacctcctgg ggaaccgtgc
     1081 atgtccaacc atcccgccat cagcctggtc gccgtgcccg gccgccgcag gcagaccatc
     1141 gagctggcgc aggagatcga gcgccgcggt ttcgccggca tctattcccc cagcatgttc
     1201 ggcaacatgt cgctgtgcga ggcgctggcg tggaacacac gcagcattcc cttcggcacc
     1261 gccatcgcgc cgatctatgc acgcaccgtc ggcgacttcg cgcagagttc ggcgttcatg
     1321 cacgaaatct ccggcgggcg cttccgcctg ggcatcggcg tggcgcacgg tccgtcccat
     1381 gtccgcatgg gcgtcacgcc gggcaagccg ctcggcgaca cccgcaattt cgtcgagcgg
     1441 ttccgcgcgc aggaagggct gggcgaactg ccgccgatca tcatcgccgc gctgcgcaag
     1501 aagatggtgg cgctggccgg ggagatcagc cagggcgtgg tgttcgccaa cgcttcgctg
     1561 tcgcacatgg cggagtcgct ggcggcgctg ccggccgcca agcgcagcga tccggccttc
     1621 ttcatcggca acatgctgcc gacctgcatc agcgacgatg tggaagcggc aaaggcggtg
     1681 tgccgccgca gcctgaccaa ctacgcattc ctgccgaatt accggaacta ctggaaggag
     1741 gccggctacg tggaggagat ggcggcgatc gaacgcgcca tcgacgaagg ccggcgcgat
     1801 gacgtgccgc gcctgttcag cgaccgctgg ctggcggaca acacgctgtt cggtccggcg
     1861 gcgaaggtgc gggagggcgt ggaagcctgg caggcagcgg gcgtgcacac gccggtgatc
     1921 gtgccgtcct cggcctccgg caaccagctg acggcactgc atgagatctt tgccgcgttc
     1981 ggttgagacc gcggcggatg caccagggat gatggcccga tgctgaatgc gttgcccgat
     2041 tccatccgcg cgcaatggcc gtaccagccg cgctatgcgc gggtgaacgg ctggcgcatg
     2101 cactacatcg acgagggcag cggcgacccg gtggtgctgc tgcacggcaa tcccacctgg
     2161 ggcttcctct accgcgactt cgtcgcgccg ctgaccgcgg cggggcaccg cgtgatcatc
     2221 ccggacatga tcggcttcgg cctgtcggaa aagcccgcgc gggaacagga ccagtcgctg
     2281 gatggccata tcgccaacct ggccgggctg ctgcgccagt tggacctgca ccgcatcacc
     2341 ctggtgtgtc acgactgggg cggccccacc gggctgggct ttgccttctc caacatggcg
     2401 cgcatgcgcg cgctggtggt gatgagcacc tggggctggc cgctgccgcc cgccgaattc
     2461 cacacgcgca tcttcccctg gcgcatgatg cacgcgccgc tgctcgcccc ctatctgctc
     2521 ggccgccaca acgcactggc ggcacgcggc
//
LOCUS       DASSZH010000081         2124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_650048, whole genome shotgun sequence.
ACCESSION   DASSZH010000081 DASSZH010000000
VERSION     DASSZH010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650048"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="VFN42_01630"
     CDS             <1..1083
                     /locus_tag="VFN42_01630"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase operon protein YhjQ/BcsQ"
                     /protein_id="HET8995346.1"
                     /translation="AIAAMQKNATPKVLIVDVSGEEQPLTALGDLSNVVEPDVCVLVI
                     GEINHVDFYREVTRGLGAADYLAKPLTRDTVARHFVPFMRGHGPATQGVLGGRAIAVT
                     GVRGGVGTTTVAVNLAWNFGVWMRRHTVLLDPDIHLGSAAFLLNIQPGSGLRMALEAP
                     ERIDSLLAERAAQPAADRLHVLGGEEKLAVQTNPAPGAAGSLLAALRRRYNFIVADVP
                     FRPMPFNRELLDQVDQRVLVMEPTLAAVRDTLRLLALPAGPEQSQRAIIVLNRVGVAG
                     GLTRRQVEDALKMKVDVAIPDLPRQVGNAATLGEPAMASSSSFRSGILELARQVAFVR
                     LLDAAPGSNPALQGRSRRWNPFRRRS"
     gene            1108..>2124
                     /locus_tag="VFN42_01635"
     CDS             1108..>2124
                     /locus_tag="VFN42_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET8995347.1"
                     /translation="MSLTFASSFGRRTTPQAAMAEAARAAAPHPAMPPAASAPQLVVP
                     APSVPNESIAELRTVCLARLDPAVVSGMPPERLTADVERLISETATERRIQLNGREQR
                     ALAEELVDDILGLGPLEPLLEDENISEIMVNGPHKTFIERGGKLVLSNVRFRDTMHLA
                     NVCQRIAAGVGRRVDESSPMCDARLKDGSRVNIVFPPLALDGPYIAIRKFGKKGVDFA
                     RLIQWGALTLQVARVLEICSRARLNIIISGGTGSGKTTMMNALSRQIEPGERVVTVED
                     TAELQLQQPHVVRLETRPASLEGRGEITQRDLVRNALRMRPDRIIIGEVRGGEAFDML
                     QAMNT"
BASE COUNT          344 a          738 c          704 g          338 t
ORIGIN      
        1 gccatcgcgg caatgcagaa gaacgccacg cccaaggtgc tgatcgtcga tgtcagcggc
       61 gaggagcagc cgctgaccgc gctcggggac ctgtccaatg tggtcgagcc ggacgtctgc
      121 gtgctggtga tcggtgagat caaccacgtc gatttctacc gcgaggtgac gcgcggcctc
      181 ggcgccgccg actatcttgc caagccactg acccgcgata cggtcgcgcg ccacttcgtc
      241 cccttcatgc gcggccacgg gccggcaacg cagggcgtgc tcggcggcag ggcgatcgcc
      301 gtgaccggcg tgcgcggcgg cgtcggcacg accactgtgg cggtgaacct ggcgtggaat
      361 ttcggcgtct ggatgcgccg ccataccgtg ctgctggacc ccgatatcca cctcggcagc
      421 gccgcattcc tgctgaacat ccagccaggc tccgggctgc gcatggctct cgaagcgccg
      481 gagcgcatcg actcgctgct cgccgagcgc gccgcgcaac cggccgccga ccggctgcat
      541 gttctcggcg gcgaggaaaa gcttgcggtg cagaccaacc ctgcgcctgg cgccgccgga
      601 tcgctgctcg ccgcgctgcg ccgccgttac aatttcatcg tcgccgatgt gccgttccgg
      661 ccgatgccgt tcaaccgcga gctgctcgac caggtggatc agcgggtgct ggtcatggaa
      721 ccgacgttgg cagcggtgcg cgacacgctg cggctgctcg cgctgcctgc cggccccgag
      781 cagtcgcaac gcgccatcat cgtgctgaac cgggttggcg tggcaggcgg cctgacccgc
      841 cgccaggtgg aagacgcact gaagatgaaa gtggatgtgg cgattcccga cctgccgcgc
      901 caggtcggga atgccgccac gctgggtgaa ccggcgatgg ccagcagcag cagcttccgc
      961 agcggtatcc tggaactcgc acgccaggtt gccttcgtcc gcctgctcga tgcggcaccc
     1021 ggttccaatc cggcattgca gggcaggtca cgccgctgga atcccttccg tcgccgcagc
     1081 taaccgcttc gccttctgga tcctgacatg tcgctgacct tcgcctccag cttcggccgc
     1141 cgtaccactc cgcaagccgc gatggcggaa gcggcgcgtg cggccgcacc gcacccggcc
     1201 atgccgcccg ccgccagcgc accccaactg gtggtgcccg ccccctcggt ccccaacgag
     1261 tcaatcgccg agttgcgcac ggtctgtctc gcgcggctgg atcccgccgt ggtgtccggc
     1321 atgccgcccg aacggctgac cgccgatgtc gagcggctga tctcggaaac cgccaccgag
     1381 cggcgcatcc agttgaacgg ccgcgagcag cgcgcgctgg ccgaggaact ggtggacgac
     1441 atccttggcc tgggaccgct ggagccgctg ctcgaagacg agaacatcag cgaaatcatg
     1501 gtgaacgggc cacataagac gttcatcgag cgcggtggca agctggtgct gtcgaatgtg
     1561 cggttccgtg acaccatgca cttggccaat gtctgccagc gcattgccgc cggcgtcggc
     1621 cggcgcgtcg atgaatcgag tccgatgtgc gacgcccgcc tgaaggacgg ctcccgcgtc
     1681 aacatcgtgt ttccgccgct cgcgctcgat ggtccctata tcgcgatccg caaattcggc
     1741 aagaagggcg tcgatttcgc caggctgatc cagtggggcg cactgaccct tcaggtcgcc
     1801 cgcgtgctgg agatctgctc gcgggctcgg ctgaacatca tcatctcggg cggcaccggc
     1861 tccggcaaga cgaccatgat gaacgcgctg tcgcgccaga tcgaacccgg cgagcgcgtc
     1921 gtgacggtgg aagacaccgc ggaactgcag ttgcagcagc cgcatgtggt gcggctggaa
     1981 acccgtcccg ccagcctgga agggcgcggc gaaatcaccc agcgcgacct ggtacgcaac
     2041 gcgctacgta tgcggcccga ccgcatcatc atcggcgagg tgcgcggcgg cgaggcgttc
     2101 gacatgctgc aggcgatgaa cacc
//
LOCUS       DASSZH010000082         4294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_428457, whole genome shotgun sequence.
ACCESSION   DASSZH010000082 DASSZH010000000
VERSION     DASSZH010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428457"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..2526
                     /locus_tag="VFN42_01640"
     CDS             19..2526
                     /locus_tag="VFN42_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HET8995348.1"
                     /translation="MPGFDSAFPARVKAVLGPTNTGKTHLAIERLLAHASGIIGFPLR
                     LLARENYDRMVARKGARYVALITGEEKIIPPEARWFSCTVEAMPLDRAAEFVAVDEIQ
                     LCADPDRGHVFTDRLLHARGMVETMFLGAETIRPLLQRLVPQAQVETRPRMSQLIYAG
                     PAKLTKLPPRTAVVAFSAQEVYAIAELIRRRRGGCAVVMGRLSPRTRNAQVALYQDKE
                     VDFLVATDAIGMGLNMNVDHVAFAGLGKFDGHRPRRLTAAEVAQIAGRAGRGMRDGTF
                     GSTADCPPLPDDMVAAVEAHSFEPLSQLCWRNSDLDFSHVDALLSTLTAPPDRPGLVR
                     GNDASDLETLATLAREPDVRHLARGRRMLRTLWDACQIPDFRKLADDTHARLCARVFG
                     HIAREGHLPTDWLAGQIAGLARADGDIDTLMQRLSGVRVWSYIAARTDWVKDAAHWQG
                     RAREVEDLLSDALHERLTSRFVDRRAAQLMRRLEAGDGEELLSAVTRRGDVVVEGHPV
                     GKVAGFSFLPDPDTQGEERKLVLRAARRALRDEMPRRVALLEAAPDEAIQLGDDQTVV
                     WDGVPVARLAAGPTVLRPVVRVLDSEFLDGAQRERLRIRLQRFLDDRIRSELAPLFRA
                     LEAGDSLVKARGLLHRLGESLGLIANDDRETVSPELRGRLRALGVRTGRLALYMPALL
                     KPRPAAMRARLWALHHGVAVPALPGPGLVSLPVPSDWPPGFAAAVGWIEAGPVLLRLD
                     IAERIAGELGWAARPGALALPPGLASRLSIKAELLPVVLRRLGFRIVPGGGLGAATYG
                     PPAPAMLVPLRRRRPPPVPAPKPRAHGPFAALAALQR"
     gene            2529..2831
                     /locus_tag="VFN42_01645"
     CDS             2529..2831
                     /locus_tag="VFN42_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HET8995349.1"
                     /translation="MAGGQEDRDWQRLDKWLWCARFMKGRSDCAALVAGGAIRINRQP
                     TDKPHARLRVGDVLTMPLRGQVRVIRVAALALRRGPATEARLLYEEIDEAVQQAQA"
     gene            2947..3690
                     /locus_tag="VFN42_01650"
     CDS             2947..3690
                     /locus_tag="VFN42_01650"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET8995350.1"
                     /translation="MPETHRNPWRGPLLGSAVSLALVLAALGGWLLGRAHSERSGGPP
                     VLFTAPAYRNLTNQNGQKVSSADFRGKVRVVTFLFPYCNTFCPIIAAHLVGFEHLLQA
                     DGLADSVQVVAFDVDPAGTGPKEMRGFLKEYGWDPQNTHWQYLTGTPKQIRTIVTGGY
                     HVDYQKVADTGPDPDPKTQAALLDGDTPQPYVANPLADKANVKYDITHENVLMLVDPE
                     GRVRVIHAQADAVSQWELLKEVKAIQDSR"
     gene            complement(3834..>4294)
                     /locus_tag="VFN42_01655"
     CDS             complement(3834..>4294)
                     /locus_tag="VFN42_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995351.1"
                     /translation="ERSSAAADGRQSSTSEPAVRPDPEETTDLDSIPTVAQFEAEIRG
                     QSVGQVLADICRDLGVSPFRCETRFDDALHQAITTYGGDHTDLCCDMLNRRIDLLAEL
                     ERTRNPDLDWPNGDDPDTVRRVVGFLVGEAPACPFGVPASPGIVEAAAPS"
BASE COUNT          622 a         1522 c         1501 g          649 t
ORIGIN      
        1 cacccaacca tccttctcat gccgggtttc gattctgcct tccccgcccg cgtcaaggcc
       61 gtccttggtc cgaccaacac cggcaagacg caccttgcga tcgaacgcct gctcgcccac
      121 gcctccggca tcatcggttt cccgctccgc ctgctggcgc gcgagaacta cgaccgcatg
      181 gtggcccgca agggtgcccg ctacgtcgcg ctcataacgg gtgaggagaa gatcatccca
      241 cccgaggcgc gctggttctc ctgcaccgtc gaagccatgc cgctggaccg cgccgccgag
      301 ttcgtcgcgg tggacgagat ccagctctgc gccgatcccg atcgtggcca cgtgttcacc
      361 gaccggctgc tgcatgcccg cggcatggtg gagaccatgt tcctgggggc cgaaacgatc
      421 cgtccgctgc tgcagcggct ggtgccgcag gcgcaggtgg aaacccgccc gcgcatgtcg
      481 cagctgatct atgccggccc ggccaagctg accaaactgc cgccgcgcac cgcggtcgtc
      541 gcgttcagcg cccaggaggt ctacgcgatc gccgagctga tccgccgccg gcgcggtggc
      601 tgcgcggttg tgatggggcg cctcagcccg cgcacccgca acgcccaggt cgcgctgtac
      661 caggacaagg aagtggactt cctggtcgcc accgacgcga tcggcatggg cctgaacatg
      721 aatgtcgatc acgtcgcctt cgccgggctg ggcaagttcg acggccatcg cccccgccgc
      781 ctcacggccg cggaggtggc gcagatcgcc ggccgcgccg ggcgcggcat gcgcgacggc
      841 accttcggca gcacggccga ctgtccgccg ctgcccgacg atatggtcgc cgcggtggag
      901 gcgcatagtt tcgaaccgct gagccagctc tgctggcgca acagcgacct ggatttcagc
      961 catgtcgacg ccctgctgtc cacgctgacc gcgccgccgg accggccggg cctggtgcgc
     1021 ggcaacgacg cctccgacct ggagacgctg gcgacactgg cgcgcgagcc ggacgtccgt
     1081 cacctcgcgc gcggccgccg catgctgcgc acgctatggg atgcctgcca gatccccgat
     1141 ttccgtaagc tggcggacga cacccatgcg cggctgtgcg cccgggtgtt cggccacatc
     1201 gcccgtgagg gccacctgcc gaccgactgg ctcgccgggc agatcgccgg gctggcgcgc
     1261 gccgacggcg atatcgacac gctgatgcag cgcctgtccg gggtgcgcgt ctggtcctac
     1321 atcgctgccc gcaccgactg ggtgaaggat gccgcacact ggcaaggccg cgcccgcgag
     1381 gtggaggacc tgctgtccga cgccctgcac gaacggctga ccagccggtt cgtcgatcgc
     1441 cgcgcggccc agttgatgcg ccggctggag gcaggcgatg gcgaggagct gctctccgcc
     1501 gtcacccgcc gcggcgacgt ggtcgtggaa ggccacccgg tcggcaaggt ggccgggttc
     1561 agcttcctgc cggatccgga cacccagggg gaggaacgca agctggtcct gcgcgccgcc
     1621 cgccgagcgc tgcgggacga aatgccgcgc cgcgtcgccc tgctggaagc cgcaccggac
     1681 gaggcaatcc agctcggcga cgaccagacc gtggtgtggg acggcgtgcc ggtcgcccgt
     1741 ctggccgccg ggccgacggt gctgcgtccc gttgtgcggg tgctggacag cgaatttctc
     1801 gatggcgccc agcgcgaacg gctgcgcatc cgattgcagc gctttctcga cgacaggatc
     1861 cgcagcgagc ttgccccgct gttcagggcg ctggaagccg gcgactcgct cgtcaaggcg
     1921 cgtggcctgc tgcaccggct gggcgagtcg ctggggctga tcgccaacga cgaccgggag
     1981 acggtttcgc cggagctgcg tggcaggttg cgcgcgctgg gggtgcgcac cggacggctg
     2041 gctctgtata tgccggccct gctcaagccg cgccctgccg ccatgcgcgc gcggctctgg
     2101 gcgctgcatc atggggtggc ggtgccggcg ttgcccggac cgggactggt gtccctgccg
     2161 gtgccgtcgg attggccgcc cggatttgcc gcagcggtcg gctggatcga agccggcccc
     2221 gtgctgctgc gcctggacat cgccgagcgg atcgccgggg aactgggctg ggcggcacgg
     2281 cccggcgcct tggccttgcc gcccggcctc gcctcacgcc tgtcgatcaa ggcggagctg
     2341 ctgccggtgg tgctgcgccg cctgggcttc cgcatcgtgc cgggcggcgg actcggggct
     2401 gcgacctacg gcccccctgc ccccgccatg ctggtgcccc tgcgccgccg ccggccgccg
     2461 cccgtgccgg cccccaagcc gcgcgcgcat ggtcccttcg cggcgctcgc cgccttgcag
     2521 cgctaagcat ggccggcggc caggaagacc gggactggca gcggctcgac aaatggctgt
     2581 ggtgcgcccg cttcatgaag ggccgcagtg actgtgccgc cctggtggcc ggcggcgcga
     2641 tccgcatcaa tcggcaaccc accgacaagc cgcacgccag gctgcgggtc ggcgacgtgc
     2701 tgaccatgcc gttgcgcggc caggttcggg tgatccgcgt cgccgccctg gcgctgcggc
     2761 gaggtcccgc caccgaggcg cgcctgctgt acgaggagat cgatgaggcg gtgcagcagg
     2821 cccaggcctg agcggccgcc gcgatgacgc cggggtgaaa acctggcaag gcggcggttg
     2881 ccgcccttgc acaccgccgc gcggcggacc tagacgctgg cgcctggctg cttcagacct
     2941 cgtcccatgc cggaaacaca ccgcaatcca tggcgcggcc cgctattggg ctctgccgta
     3001 tcccttgcct tggtcctcgc cgcattgggc ggctggctgc tcggccgcgc gcacagcgaa
     3061 cgaagcggcg gtccgccggt gctgttcacc gcgccggcct atcgcaacct gaccaaccag
     3121 aacggccaga aggtctcctc ggccgacttc cgcggcaagg tgcgggtggt caccttcctg
     3181 tttccgtact gcaacacgtt ctgtccgatc atcgcggcgc acctggtcgg gttcgagcac
     3241 ctgctgcaag ccgacggcct cgccgacagc gtgcaggtgg ttgccttcga cgtggatccc
     3301 gccggaaccg ggcccaagga gatgcgcggc ttcctgaagg aatacggctg ggacccgcag
     3361 aacacccact ggcaatacct gaccggcacg ccgaaacaga tccgcacgat cgtcaccggc
     3421 ggataccacg tggactacca gaaggtggcc gataccggcc ccgatccgga cccgaagacc
     3481 caggcggcgc tgctcgacgg tgacacgccg cagccctacg tggcgaaccc gctcgccgac
     3541 aaggccaacg tgaaatacga catcacgcat gagaacgtac tgatgctggt cgaccccgag
     3601 ggccgggtgc gggtgatcca tgcgcaggcc gacgcggtat cccagtggga actgctgaag
     3661 gaggtcaagg cgattcagga ttcgcggtga gatcgtcacc gcctcggacg gcggcagcga
     3721 aatcggcggc ctgtctgcgc ctgcgggcct gatggcatcg gcggaatccg gacggcgggg
     3781 ccggccggtg tccggtcccg tgcgtcatcg atgcggttgg cctgcgcgcg ccgtcaggac
     3841 ggtgcggcgg cctccacgat tcccggtgat gccggcacgc cgaaaggaca ggccggcgcc
     3901 tcaccaacga ggaatcccac gacgcgccga acggtgtccg gatcgtcgcc gttcggccag
     3961 tcgagatcgg gattccgggt gcgctccagt tccgccagca gatcgatcct gcggttcagc
     4021 atgtcgcagc aaagatcggt gtgatcgccg ccataggtgg tgatcgcctg gtgcagcgcg
     4081 tcgtcaaacc gggtttcgca gcggaacggc gaaacgccga ggtcgcggca gatatcggcg
     4141 agcacctggc caacggattg gccgcggatc tccgcttcaa actgcgcgac ggtgggaatg
     4201 ctgtcgaggt cggtggtttc ctccggatcg gggcggacag cgggttcgga cgtggatgat
     4261 tgacgtccgt ccgctgccgc ggatgaccgc tccc
//
LOCUS       DASSZH010000083         3409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_462458, whole genome shotgun sequence.
ACCESSION   DASSZH010000083 DASSZH010000000
VERSION     DASSZH010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462458"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1438
                     /locus_tag="VFN42_01660"
     CDS             <1..1438
                     /locus_tag="VFN42_01660"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET8995352.1"
                     /translation="QACPSWAMTGPLHPFLTIAEAGRLIAAKRLSPVELVKELLARIE
                     AKDPQLSAFLLVTERQALAAARAAERAVMAGKRSPLLGIPLAYKDIYETAGVRTTAHS
                     RILADNVPAQDAETVRRLHAAGAVMLGKLATHEFAIGGPATDLPWPPARNPWDTRRFT
                     GGSSSGSGAAVAAGLTLGALGSDTGGSIRLPASYCGIAGLKPTPGLVSRRGVIPLAPS
                     LDTAGPMAWTAQDCAILLDALAGHDPADPASVPGPAVSYARGLDAPLKGLRVGLLRRF
                     YEHDVPATPEMLQMMSRAAATFRQLGCRVEEAVLPPVQDYNAVGRVIICAEAYALHEA
                     TLRTRLSDYSRVFRIRVLAGALIRAADYIAAQRHRSDLISATAKVLEKFDVLVSMPTP
                     STAPLMSEQRPDDGFSRPLLTTVANVAAVPSLVVCGGFSATGLPMGIEIMGRPWEDAT
                     VLRAGHQFEQAAGTRTLRPQALWMGAGA"
     gene            complement(1671..2870)
                     /locus_tag="VFN42_01665"
     CDS             complement(1671..2870)
                     /locus_tag="VFN42_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8995353.1"
                     /translation="MAHRGLKFGIFLAPFHRVGENPTLAIARDVELLQWLDWLGYDEA
                     WIGEHHSAGWELIAAPELIIAAAAEKTRHIKLGSGVTSLPYHHPLMVAQRFVQLDHMT
                     RGRAMLGCGPGALTSDAYMLGIEPSTQRPRMLEAMDAIMRLLKCEEPVTMKTDWFEMR
                     DARLHLRPYTDPHFEIAVASTITPFGMIAAGTHGVGVLSIGAGLPGGPEALATQWKIA
                     EDAAAKSGKTVDRRNWRVVVNAHIAEDDEQALAEVKKGERVETVTYFEDTLGRPPGRA
                     DDPLRDGVKMGSTLVGSPETVIKGIHRLLEYSKGGFGGILFRAHEWANREQTLRSYEL
                     FARYVMPHFQASLDTIVGSNTWARENRKQVFGATPEAVKRAFTDFGHAVPEEFRSRTI
                     GARDQDR"
BASE COUNT          503 a         1156 c         1182 g          568 t
ORIGIN      
        1 gcaagcctgt ccgtcgtggg cgatgacagg ccccttacac cccttcctta ccatcgccga
       61 ggcaggccgc ctgatcgccg cgaagcggct gtcgccggtg gaactcgtga aggagttgct
      121 ggcccggatc gaggccaagg acccacagct gagtgccttc ctgctggtga ccgagcgcca
      181 ggcgctcgcc gccgcccgcg ccgccgaaag ggccgttatg gccgggaagc gatcgccgct
      241 tctcggcatc ccgctcgcct acaaggacat ctacgaaaca gccggcgtgc gcaccacggc
      301 gcattcccgc atcctggccg acaacgtgcc ggcgcaggac gccgagacgg tgcgccgcct
      361 gcatgcggcc ggtgcggtga tgctgggcaa gctggcgacg catgaattcg ccatcggcgg
      421 gccggcgacc gacctgccct ggccgccggc ccgcaatccc tgggacacgc ggcgcttcac
      481 cggaggctcc tcctccggct ccggcgcggc ggtcgcggcc gggctgacgc tgggcgcgct
      541 gggttccgac accggcgggt cgatccggct gccggcttcc tattgcggca ttgccgggct
      601 gaagccgacg cccgggctgg tgtcgcgccg cggcgtgatt cccctggcgc ccagcctgga
      661 cactgccggc ccgatggcgt ggaccgcgca ggactgcgcc atcctgttgg atgcgctggc
      721 gggacacgac ccggctgatc cggcctcggt ccccggtccg gcggtgtcct atgcgcgcgg
      781 cctggacgcg ccgctgaagg gtctgcgtgt ggggctgctg cggcgcttct atgagcacga
      841 cgtgcccgcc acgccggaaa tgctgcagat gatgagccgg gccgccgcga cattccgcca
      901 gttgggatgc cgggtggagg aggccgtgct gccgccggtg caggactaca acgcggtggg
      961 gcgggtgatc atctgcgccg aggcctatgc gctgcacgag gcgacgctgc gcaccaggct
     1021 gtccgactat tcgcgcgtct tccgcatccg cgtgctggcg ggcgcgctga tccgggccgc
     1081 cgactacatc gccgcgcagc gccaccgcag cgacctgatc agcgccaccg ccaaggtgct
     1141 ggagaagttc gacgtgctgg tcagcatgcc cacgccctcc accgccccgc tgatgagcga
     1201 gcagcggccg gatgacggct tctcccgccc gctgctgacg acagtggcga atgtcgcggc
     1261 ggtgccgtcg ctggtggtgt gcggcggctt cagcgccacc gggctgccga tgggcatcga
     1321 gatcatgggg cgtccctggg aggacgccac cgtgctgcgc gccggccacc agttcgagca
     1381 ggccgccggc acccggacgc tgcggccgca ggcgctgtgg atgggtgccg gcgcttagaa
     1441 attgcgcaca aacaggggat ccaatcggcg tgggcgcgac acacgtgtca tggccgggct
     1501 tggcccggcc acccacgttt ggccttgttg tcagcagcga agtcgtgggt ggccggcctt
     1561 cgccggccat gacacaaatg acgcgagccg cctggaaccg actcggttat tgcgcaacgg
     1621 cacctcagta cgtcccgcca cgaccacgtc gtcgcgcggt tcgcccgcgt ttaccgatcc
     1681 tggtcgcgcg cgccgatggt gcgcgagcgg aattcctccg gcaccgcgtg gccgaaatcg
     1741 gtgaaggcgc gcttcaccgc ttccggcgtg gcgccaaaca cctgcttgcg gttctcgcgc
     1801 gcccaggtgt tggagccaac gatggtgtcc aatgacgcct ggaaatgcgg catgacataa
     1861 cgagcgaaca gctcatagct gcgcagcgtc tgctcgcggt tggcccattc atgcgcacgg
     1921 aacaggatgc cgccgaatcc gcccttgctg tactccagca ggcggtggat gcccttgatc
     1981 accgtttccg gcgagccgac cagcgtggac cccattttca cgccgtcccg cagcggatcg
     2041 tcggcacggc cgggcggacg cccgagcgtg tcctcgaaat aggtgacggt ttccacgcgc
     2101 tcgcccttct tcacttcggc cagcgcctgt tcgtcatcct cggcgatatg cgcgttcacc
     2161 accacgcgcc agttgcggcg gtccaccgtc tttccgctct tcgcggcggc gtcctcggcg
     2221 atcttccatt gcgtggcgag cgcttccgga ccgccgggca gccccgcgcc gatcgacagc
     2281 acgccgacac cgtgcgtgcc ggccgcgatc atgccgaagg gggtgatcgt gctggcgacg
     2341 gcgatctcga aatgcgggtc ggtgtagggg cgcagatgca ggcgcgcgtc gcgcatctcg
     2401 aaccagtcgg tcttcatggt gaccggctct tcgcatttca gcaggcgcat gatggcatcc
     2461 atcgcttcca gcatgcgcgg gcgctgggtg gatggttcga tgcccagcat atacgcgtcc
     2521 gaggtcagtg cgcccgggcc gcagcccagc atcgctcggc cgcgcgtcat gtggtcgagc
     2581 tgcacgaagc gctgtgccac catcagcggg tggtggtacg gcaggctggt gacgcccgag
     2641 ccaagcttga tgtgacgggt cttttccgcg gcggcggcga tgataagctc cggtgcggcg
     2701 atcagttccc agccggcgga gtggtgttcg ccgatccagg cttcgtcgta gccgagccag
     2761 tcgagccatt gcagcagctc gacatcgcgg gcgatggcaa gggtcgggtt ctcgccgacg
     2821 cgatgaaagg gggcgaggaa gattccgaac ttcagtccgc ggtgcgccat cggcattctc
     2881 catgggtcgg gccgtacagg cccgcatggt gcgctgatac gggggcgagg ccaagcctcc
     2941 cgcctcgcgc attccgcccc gcggcatgtg ggattcgcag ctccgcattc cgcgcagaga
     3001 cgcagaggac cgcagagttc gcagacagcg tcgcggaatg aaacggtcga tgccgtcttt
     3061 cggcaaggcg gcgttgaagc tgatcggcaa gccaatcctg ccagcaaccc aggcccgggc
     3121 tggcggtaca cgtacatcgt accggcaatg acgcggccag tcaggtccgc atcattctct
     3181 gcggttctct gcggttctct gcgtctctgc gcggaatgcg gtattgctgc catctccagc
     3241 gctgccctgt tggccgcgag agcgaaggta cggcatgacg ggggaggctt ccgtcccgcg
     3301 gcctgtgggg tccgcagctc cgccttccgc gcagagacgc agaggaccgc agagttcgca
     3361 gacggcgtca ggggatgaag cggtggatgc cgtctttcag caggacggt
//
LOCUS       DASSZH010000084         2533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_616063, whole genome shotgun sequence.
ACCESSION   DASSZH010000084 DASSZH010000000
VERSION     DASSZH010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616063"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VFN42_01670"
     CDS             complement(<1..494)
                     /locus_tag="VFN42_01670"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HET8995354.1"
                     /translation="MPLLFSFVSLALLAVAAWRDVAARVIPDAVPAALLLVGLAWRIA
                     LAGLPPAGMSLGLALALFTALVALHAAGWLGGGDVKLAAGFAAGLAPQAVLPFLAATA
                     IAGGVLAGLHLAVRGLPFRRRRPAPGFLLWRVVRAEHWRIARHGSLPYGVAIACGGAW
                     IILVG"
     gene            complement(1124..1294)
                     /locus_tag="VFN42_01675"
     CDS             complement(1124..1294)
                     /locus_tag="VFN42_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HET8995355.1"
                     /translation="MTYALQTALAALKDRKGVTALEYGVLAAGIIAVIAAVVFTIGSN
                     LTTIFSDVNNKL"
     gene            1806..>2533
                     /locus_tag="VFN42_01680"
     CDS             1806..>2533
                     /locus_tag="VFN42_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995356.1"
                     /translation="MRLAGGAALALIGATAVRPAEAGYQAGYVAQFQGANLSNSGFIP
                     PDMGGAVGNGYVLQMLNGAVSTYATSGTLVGSRQSLNAFWSGAGLGTAIGGTSVSDPR
                     VIFDPSSGHWFASSISTQGTNNNILVAVSRTSDPTQGFVGYAIPAAGNNFADFPTLGV
                     NNSAVTIGTNNFTSSTGSLVGSSVYSISKSALIDSIGSAQPPSVTRFDNTSGVGFTPQ
                     AVSNLTGTGTTTTLLSSNTATTFNV"
BASE COUNT          500 a          906 c          757 g          370 t
ORIGIN      
        1 ccgacaagga tgatccaggc gccaccacag gcaatcgcga cgccgtaggg cagcgacccg
       61 tggcgggcga tacgccagtg ctcggcgcgg accacacgcc acaacaggaa gccgggcgcc
      121 ggccggcgcc gccggaacgg cagcccgcgg acggcgagat gcagccccgc cagcacgcca
      181 ccggcgatgg cggtcgcggc gaggaaaggc aggaccgcct gcggggcgag accggcggca
      241 aagccggcgg cgagcttcac gtcgcccccg ccgagccagc cggccgcatg gagtgcaacg
      301 agagcggtga acagggccag cgcgaggccg agcgacattc cggccggcgg cagccccgcc
      361 agcgcgatcc gccatgccag gccgaccagc agcaacgccg ccggcaccgc atccgggatc
      421 acccgtgccg ccacatcgcg ccacgcggca accgccagca gcgcaagcga cacgaaggag
      481 aacagcaacg gcatcgcccg gcccccgtcc ccgaagattg cgatccagtc accccgcctg
      541 ggctgagtcc gggaagaggc gcatggccgc gccatcccgt cgcagcacgc cgcgtgcccg
      601 gacagcgttc gcacggtcag ggcgagtcgt cgtcgtggcg cgcaaaagcc gcgctcacca
      661 gatcaaccca accgggaagc ccagctcgcg gcgcgcaact gagcatcgaa acccccttcc
      721 tgccgcggac cgacccgact cgcccgccgt caccgatccg accactctcc caagccccac
      781 gcccgccaaa cgtttggttt tcgtatggaa aacccccttg tcgggatgcc tccgggcctg
      841 ctactcgcac gcccgctatt cctggacgcc ctggaatgac ttgccgcaca aataatacgg
      901 aagtcatttc tgcggcgcga cgcaccccgt ggacacctgg acaccccacc ccggacgcac
      961 tgccggcgcc cggagtggtt caggaccgtc gatgacagga ccgatgccgc cggccgatca
     1021 gcaggccgcg cgccgcggtc cggcaccgaa cggagcaccg accacgagcg cgccatcgat
     1081 aactgtcatc gacctgaacc atcgcaaccg atgcacccaa tcgtcagagt ttgttgttca
     1141 cgtcgctgaa aatcgtggtg agattgctgc caatcgtgaa caccacggcc gcgatcacgg
     1201 cgatgatccc cgccgcgagc acaccatatt ccagagccgt cacccccttg cgatccttca
     1261 gggccgccag ggcagtctgc agcgcgtatg tcaacatcga accaaccttc cccttatcgt
     1321 tagcaggcgg cccggccgcc cacgatttca gtcgagagat gcggcagccc caccgcgacc
     1381 tttccagcag cagacctttc ccgcagcgca cagactatga cgcatatccg gtgagtgcaa
     1441 gagtaacgaa tatgcgattt gcaaaacaca cacatataat ctgcaaactt aatcgaaacg
     1501 tgttggtcat accaattgat ttttcgatac cgatccgact tgtcgcgaag tgttagtaat
     1561 tatttggtat taatatcttc ctgtgtccgt cgttcgccga gcatgcgggc ggcagacttc
     1621 gccgccgccg atcgcttgac ggcccgacaa aacgacggaa ctattgtgag accgcatcgg
     1681 acaggggcgc gcgcagcgac ctgcgtggcc ctgccgcggg taacaagggg gctgcacgag
     1741 caatgggacg cacaacgtac ccggatcagc cgggtcacgc gacggcacgt gcgcgcgggg
     1801 aacggatgcg ccttgcgggc ggcgccgcct tggcgctgat cggcgcgacc gcggtgcggc
     1861 cggccgaggc cgggtatcag gccgggtatg tggcgcagtt ccagggcgcg aacctgtcga
     1921 attccggctt catcccgccc gacatgggcg gcgcggtcgg caacggctac gtgctgcaga
     1981 tgctgaacgg ggcggtcagc acctatgcca cttccggcac gctggtcgga tcacggcaat
     2041 cgctcaatgc cttctggtcc ggtgccgggc tgggcaccgc gatcggcggc acctcggtat
     2101 ccgatccccg cgtgatcttc gatccctcca gcgggcattg gttcgcgagt tcgatctcga
     2161 cacagggcac caacaacaac atcctggtcg ccgtctccag gaccagcgat ccgacgcagg
     2221 gattcgtcgg ctacgccatc ccggccgcag gcaacaactt cgccgacttt cccaccctgg
     2281 gcgtgaacaa ttccgccgtg accatcggca ccaacaattt caccagcagc accggttcgc
     2341 tggtcgggag ttccgtctac tcgatctcga aatccgccct tatcgacagc atcggctcgg
     2401 cgcagccgcc ttccgtcacg cggttcgaca atacatcagg cgttgggttc accccgcagg
     2461 cggtcagcaa tctgacggga accggcacca cgaccacgct gctcagcagc aacaccgcca
     2521 ccaccttcaa cgt
//
LOCUS       DASSZH010000085         2156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_360103, whole genome shotgun sequence.
ACCESSION   DASSZH010000085 DASSZH010000000
VERSION     DASSZH010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360103"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1763)
                     /gene="dxs"
                     /locus_tag="VFN42_01685"
     CDS             complement(<1..1763)
                     /gene="dxs"
                     /locus_tag="VFN42_01685"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HET8995357.1"
                     /translation="METKLLDRVRYPADLRNFSAEQLRQLADELRAETIDAVSVTGGH
                     LGASLGVVELTVAIHAVFDTPYDRLIWDVGHQAYPHKILTGRRDRIRTLRQGGGISGF
                     TRRSESEYDPFGAAHSSTSISAGLGMAVARDLKDARGEQDGRNVIAVIGDGAMSAGMA
                     YEAMNNAGALRSRLIVILNDNDMSIAPPVGAMSAYLSRLMSSRSFMSLRDLGSRMAKR
                     FPRGIERTLGRAEEYARGMLTGGTLFEEMGFYYLGPIDGHNLDHLLPVLRNVREAEES
                     GPILIHAITQKGKGYAKAEESADKLHSVSKFNVITGEQAKAPPGPPNYTNVFAQALID
                     EAKRDPNIVAITAAMPSGTGLDKFEKAFPDRCFDVGIAEQHAVTFAAGMATEGMKPFC
                     AIYSTFLQRAYDQVVHDVAIQSLPVRFAMDRAGPVGNDGATHSGSFDLAYLGCLPGFV
                     LMAPSDEAELVHAVATAVQIDDRPSGLRYPRGAGVGVALPKRGEAWQIGKARILREGN
                     NIAILSLGTRLGDAMKAADELAAHGLPATVADARFAKPLDTALIEQLARHHEVLITIE
                     EGSIGGFSSAVLHHLATNGLL"
     gene            complement(1795..>2156)
                     /locus_tag="VFN42_01690"
     CDS             complement(1795..>2156)
                     /locus_tag="VFN42_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003620236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET8995358.1"
                     /translation="LQALKTGRLIQYSAEAGAILGRAPLPQRHVLAAYGRDLGAAFQI
                     ADDLLDAEGTTQETGKTAGKDAAAGKATMVAILGPERARAHAELLARQAAGHLDSFGD
                     KASLLRSLATYVVTRRN"
BASE COUNT          333 a          730 c          724 g          369 t
ORIGIN      
        1 tccagcaggc cgttggtcgc caggtggtgc agcaccgccg agctgaaacc gccgatcgaa
       61 ccttcctcga tcgtgatcag cacctcgtgg tggcgcgcca gctgttcgat cagcgcggta
      121 tccagcggct tggcgaagcg cgcgtccgcc acggtggcgg gcaggccgtg cgcggccagt
      181 tcgtccgccg ccttcatggc atcgcccagg cgggtgccga gcgacaggat ggcgatgttg
      241 ttgccttcgc gcaggatacg ggctttgccg atctgccaag cttcgccgcg cttgggcagc
      301 gccacgccaa cgcccgcacc gcgcggatag cgcagcccgc tcgggcggtc atcgatctgc
      361 accgcggtgg cgaccgcgtg taccagttcc gcctcgtcgc tcggcgccat cagtacgaag
      421 ccgggcaggc agcccagata ggccagatcg aagctgccgg aatgggtcgc gccgtcattg
      481 ccgaccggac cggcgcgatc catggcgaag cgcaccggca gcgactggat cgccacgtcg
      541 tgcaccacct ggtcgtacgc gcgctgcagg aaggtcgagt agatcgcgca gaacggcttc
      601 atcccctcgg tggccatgcc ggccgcgaag gtcaccgcgt gctgctccgc gatgccgaca
      661 tcgaaacagc ggtcgggaaa cgccttctcg aacttgtcca ggccggtgcc ggacggcatc
      721 gccgcggtga tcgccacgat gttgggatcg cgcttcgcct cgtcgatcag cgcctgggcg
      781 aacacgttgg tgtagttcgg cggtcccggc ggcgccttgg cctgctcgcc ggtgatcacg
      841 ttgaacttgg agacgctgtg cagcttgtcg gcggactcct ccgccttggc gtagcctttg
      901 cccttctgag tgatcgcatg gatcaggatc gggccgcttt cctctgcttc gcgcacgttg
      961 cgcagcaccg gcagcaggtg gtccaggttg tggccatcga tcgggcccag gtagtagaag
     1021 cccatctcct cgaacagcgt gccgccggtg agcatgccgc gggcgtactc ctcggcgcgc
     1081 cccagcgtgc gctcgatgcc gcgcgggaag cgcttggcca tgcgggagcc aaggtcgcgc
     1141 agcgacatga agctgcggga ggacatgagg cgcgacaggt acgcgctcat cgcgccgacc
     1201 ggcggcgcga tcgacatgtc gttgtcgttc aggatgacga tcaggcggga gcgcagggcg
     1261 cccgcattgt tcatcgcctc atacgccatg ccggcgctca tggcgccgtc gccgatcacg
     1321 gcgatcacgt tgcgcccgtc ctgctcgcca cgcgcatcct tcaggtcgcg cgccacggcc
     1381 atgcccaggc cggcggaaat cgaggtggag gaatgggcag caccgaacgg gtcgtactcg
     1441 ctctcgctgc ggcgggtgaa gccggagatg ccaccgccct ggcgcagggt gcggatgcgg
     1501 tcacgccggc cggtcaagat tttgtgtgga taggcctggt ggccgacgtc ccagatcagc
     1561 cggtcgtacg gggtgtcgaa cacggcatgg atcgccaccg tcagctcgac cacgcccagc
     1621 gaggcgccga gatggccccc ggtgacggaa accgcatcaa tcgtctcggc ccgcagctca
     1681 tcggcgagct ggcgcaactg ctcagcggag aagttgcgaa gatcggccgg atagcgcacc
     1741 cgatccagga gtttggtttc cacaggatct ccttttctct ggctcgcccg tgcttcagtt
     1801 gcgacgcgtt accacatagg tggccagtga ccgcaacaaa gacgctttgt cgccgaaact
     1861 gtccaggtgg ccggcagcct ggcgggcaag cagttcggca tgggcccgcg cccgttccgg
     1921 tccgaggatc gccaccatcg tcgcctttcc agctgcggca tccttgcccg cggtcttccc
     1981 ggtctcctgc gtggtaccct ccgcgtccag tagatcatcc gcgatctgga aggcggcgcc
     2041 cagatcacgc ccataagccg ccagcacgtg gcgctgcggc aacggtgccc gcccgagtat
     2101 cgcgccggct tcggcgctgt attggatcaa ccggccggtt ttcagcgcct gcagcc
//
LOCUS       DASSZH010000086         2679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1621723, whole genome shotgun sequence.
ACCESSION   DASSZH010000086 DASSZH010000000
VERSION     DASSZH010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621723"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1026
                     /gene="yghU"
                     /locus_tag="VFN42_01695"
     CDS             154..1026
                     /gene="yghU"
                     /locus_tag="VFN42_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HET8995359.1"
                     /translation="MSDAAEYVPPKVWQPKPALGRFANINRPVSGATHEKELPVGKHP
                     LQLYSQGTPNGVKVTVMLEELLAAGHKGAEYDAWLIRINDGDQFGSGFVAINPNSKIP
                     ALVDRSGPKPFRVFETAAILVHLAEKFGAFLPTDPAKRAECFSWLFWQMGSAPYLGGG
                     FGHFYAYAPVKIEYAIDRFTMETKRLLDVLDKRLAESEWVAGSEYTIADIACWPWYAG
                     LAKFGQYGATEFLSVHEYRNVQRWADTIWARPAVKRGRMVNRTQGDPSEQLWERHDAS
                     DFETKTQDKVAKAG"
     gene            1178..1498
                     /locus_tag="VFN42_01700"
     CDS             1178..1498
                     /locus_tag="VFN42_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET8995360.1"
                     /translation="MHPIVTARRDAIAELCRRFHVRRLEVFGSAARGEDFDPQRSDAD
                     FLVEFESDTPPTIGLLLDLEVALADTLGRSVDLAERKAIEEGGNYLRRRRIMEEAEPV
                     YVAG"
     gene            1485..1832
                     /locus_tag="VFN42_01705"
     CDS             1485..1832
                     /locus_tag="VFN42_01705"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HET8995361.1"
                     /translation="MSRDEALLLDMFLAASEAIQFVAGLDQLQFAGSRLHQAAVIRSL
                     EVIGEAASRVSTPLREAHSEIPWREMIGMRNRLIHAYARVSVDVVWNVARHRLPELVA
                     ALQPLVPAPGDEV"
     gene            1934..2254
                     /locus_tag="VFN42_01710"
     CDS             1934..2254
                     /locus_tag="VFN42_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995362.1"
                     /translation="MIDIDGLNEAELRDLNHRIVARLRLLRDVKAHEAMLAFRVGDRV
                     SFESASGTVTGVITRYNRKTVGVMADSGHQWNVSPSYLRKAGPPARNGAGPVIDGEVL
                     RPAD"
     gene            complement(2285..>2679)
                     /locus_tag="VFN42_01715"
     CDS             complement(2285..>2679)
                     /locus_tag="VFN42_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014104615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET8995363.1"
                     /translation="GLAATLIWHEAPHRLAETLADMADLFGARSAAVARELTKRFEEV
                     RRGSLADLAAHYAANAVRGEVTVLVGPPEQADPEDLDGRLRAALSTQSVKDAAALVAA
                     ATGLPRKLVYARALELSQGGGSASGGGG"
BASE COUNT          441 a          896 c          926 g          416 t
ORIGIN      
        1 gttgctgcga ccgcccgaag ggctgccgtc gcaagccgct gccgcgtcgg tgctggcact
       61 gctggaccgg ccggcgcgct gacgcaggcc gggttgcgcg cggccggcac ccgggccacg
      121 gtgcgagcca acattcatcc ggagggttcc atcatgtccg acgccgccga gtacgtcccg
      181 cccaaagtct ggcagccgaa gccggcactg ggccgcttcg ccaacatcaa ccgcccggtg
      241 tcgggtgcaa cgcatgagaa ggaactgccg gtcggcaaac atccgctgca gctctattca
      301 cagggcacgc cgaatggcgt gaaagtgacg gtgatgctgg aggagctgct ggccgccggc
      361 cacaagggcg ccgaatacga cgcctggctg atccgcatca acgatggcga ccagttcggc
      421 agcggcttcg tggcgatcaa tcccaattcg aagattcccg cgctggtgga ccgcagcgga
      481 ccgaagccgt tccgcgtgtt cgagaccgcc gccatcctgg tgcacctggc ggagaagttc
      541 ggcgccttcc tgccgaccga tccggccaag cgggcggagt gcttttcctg gctgttctgg
      601 cagatgggca gcgcgccgta tctcggcggc ggcttcggcc atttctatgc ctatgcgccg
      661 gtgaagatcg aatacgcgat cgaccgcttc accatggaga ccaagcggct gctggatgtg
      721 ctggacaagc gcctggcgga atccgagtgg gtggcgggca gcgaatacac catcgccgat
      781 atcgcctgct ggccgtggta cgccgggctg gcgaagttcg ggcagtacgg cgcgaccgag
      841 ttcctgtcgg tgcacgagta caggaacgtc cagcgttggg ccgacacgat ctgggctcgg
      901 ccggcggtga agcgcgggcg gatggtgaac cgcacgcagg gcgacccgtc cgagcagctt
      961 tgggaacggc acgacgccag cgacttcgag acgaagaccc aggacaaggt ggcgaaggcg
     1021 gggtagaaga accggaacgg cggagggcgc ggtgcaatcg accgcgccgc cgaggcccag
     1081 gccgccgccg gcacaaggcg gtgggagttg cgaatggctt gctgtagtgt gcggccatcc
     1141 caacacgcga tgccgccgtg aacggccgga cccgatcatg cacccgattg tcaccgccag
     1201 gcgcgatgcc attgccgaac tctgccgccg cttccacgtg cggcggctgg aggtattcgg
     1261 ctccgccgcc cgcggcgagg acttcgaccc gcagcgtagc gatgccgatt ttctcgtcga
     1321 gttcgagtcg gacacaccgc caacgatcgg tctgttgctc gaccttgagg tggcacttgc
     1381 ggataccctg ggccgatcgg tggatctggc ggagcgcaag gctatagagg aaggcggcaa
     1441 ttacctgcgt cgccgccgga tcatggagga agcagagccg gtctatgtcg cgggatgagg
     1501 cgctgctgct cgacatgttc ctcgccgcat ccgaagcgat ccagttcgtg gccgggctcg
     1561 atcagcttca gttcgcgggg agcagactcc atcaagctgc ggtgatccga agcctggagg
     1621 tgatcgggga agccgcatcc agagtttcga cgcccctgcg tgaggcgcat tccgagattc
     1681 cctggcgcga aatgattggt atgcgcaacc gccttatcca tgcttatgca cgcgtcagcg
     1741 tggacgtagt gtggaacgtt gcgcggcatc ggctgcctga gctggtcgca gcgctccagc
     1801 cgctggtccc agcgccgggt gatgaggtct gaaccgagtc gcaggcgcat ttgctcgcca
     1861 acttcgcttc atgacatcgg ccgcgcgttc cagcagaatg ccgcccccga atcacccttg
     1921 ggcggacgct gcgatgatcg acatcgacgg cctgaacgaa gccgaactgc gcgatctcaa
     1981 ccaccgcatc gtcgcgcggc tgcgcctgct gcgggacgtg aaggcacacg aggcgatgct
     2041 ggcgttccgc gtcggcgacc gcgtcagctt cgagtccgcc agcggcaccg tgacgggcgt
     2101 gatcacccgc tacaaccgca agaccgtcgg cgtgatggcc gattcgggcc atcagtggaa
     2161 tgtctccccc agttacctgc ggaaggccgg gccgccggcc cgcaacggcg cggggccggt
     2221 aatcgacggt gaggtgctgc ggccggcgga ctgagcgcat ccgcccgccg gaccggccgg
     2281 tccgctaccc gcccccgccg gacgccgaac cgccgccctg gctcagttcc agcgcccgcg
     2341 cgtagaccag cttgcggggc aggccggtcg cggcggcgac cagggcagcg gcgtccttga
     2401 cgctctgggt tgacagggcg gcccgcaggc ggccgtcgag gtcctcgggg tcagcctgtt
     2461 ccggcggccc gaccagcacc gtcacctcgc cgcgcaccgc gttcgcggcg taatgcgccg
     2521 ccaggtccgc cagcgaaccg cgccgcacct cctcgaaccg cttggtcagt tcccgcgcca
     2581 ccgcggcgga gcgggcaccg aacaagtccg ccatgtcggc cagcgtctcc gccagccggt
     2641 gcggcgcttc gtgccagatc agcgtcgccg ccaggccgg
//
LOCUS       DASSZH010000087         3926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1110410, whole genome shotgun sequence.
ACCESSION   DASSZH010000087 DASSZH010000000
VERSION     DASSZH010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110410"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /locus_tag="VFN42_01720"
     CDS             <1..1277
                     /locus_tag="VFN42_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HET8995364.1"
                     /translation="FTVTSMLCGIATSLTEMVLFRLLQGVFGAALVPLSQAVMLDIYP
                     AKQRGSAMAIWGMGVMVGPILGPTLGGYLTEFYNWRFVFFVNLPFGLAATIGLLLFLP
                     RTEPQESRFDWTGFLMFAIGIAGLQMMLDRGETKDWFGSTEIIVEAVMAGLGLYLFVV
                     HMFTAKKPFITPRIFKDRNLNAGLLVMFAVGLVLLAVSALLAPWLQTLGNYPVETTGL
                     MMAPRGIGTMAAMMIAGRLTNKVDPRALMAFGILVLSWSLYRMTGWTPAVNETEFVVN
                     TIVQGFGLGFVFIPLQVIAFATLEPMLRTEGTALLSLTRNVGSAVGISVTEALLTHNT
                     QIEHDSLSKFASPLNRAFQSLGPMGHALAPTTSQGQQILNGMINQQAQIIAYNDDYKL
                     MMLTALPMLFLLLLMRRPRGGNAGGDHPAVLD"
     gene            1376..2533
                     /locus_tag="VFN42_01725"
     CDS             1376..2533
                     /locus_tag="VFN42_01725"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8995365.1"
                     /translation="MSSSRTAGSRSWSLAAAISAVAVFGLGVGQFAPLLSLLLDARGV
                     TPTLNGLNAATSSIGIVLGPLLAPRGVRALGVNRFLMLCFVLDIILVLALKLFDTVAA
                     WFVLRFLLGLVGSNIFTASEAWINRLAGDAGRGRVLGLYAALLSAGFGVGPLLLVLTG
                     VHGWWPFAINAAITAIALLPLLAAGGGMLDLGREQSGSSIAAARRAPMIFVTVALFGL
                     FEFALMALLPVWALRHGMDARQAAGTLSGVYIGAIALQVPVGWLSDRLSRRAALRLCG
                     TVGLSGAAVLFAVPLSGPALFAFLFLWGGTVAAIYPVALSMAGDRFPPGEMVAMNAAV
                     ISAYGIGSLIGPALGGAAMDTWNSQGLPGMFILLFALFLLLTFTRAHRPGT"
     gene            2606..3187
                     /locus_tag="VFN42_01730"
     CDS             2606..3187
                     /locus_tag="VFN42_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase"
                     /protein_id="HET8995366.1"
                     /translation="MRLPFRITVCGIDELSGHCEAGVSHVLSILDPGHPEPSAFGSFG
                     EHVRLELRFNDIIEEAPDMQMPSRLHVEQLLAFGRTLPTEAQAHLLVHCHAGISRSSA
                     SMTLLLAQAMPDCPAAAIFDEVLRIRPIIWPNLRILELGDAALRRDGALIEAAAGLYG
                     RQLERRPELSELYRGNGRGREVQMGRMDGVTNG"
     gene            complement(3239..>3926)
                     /locus_tag="VFN42_01735"
     CDS             complement(3239..>3926)
                     /locus_tag="VFN42_01735"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010513864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HET8995367.1"
                     /translation="TGVNTPRGKNLVGAFHQPRMVLADTATLATLPVRELRAGYAEIV
                     KAGLIGDPAFFRWCEQHGAGVVGGDREAQAEAIKRACAFKAAVVGDDEREEKPNDGRA
                     LLNLGHTFGHALEAEYGYTGGLLHGEGVAVGLGLAFKLSARLGHCSSADAERVVAHVA
                     GAGLPAELSMLNRRFSAATLIGHMRRDKKVRDGALNFVLVRGIGQAFTSADVPMDAVV
                     DLLRDEGCTE"
BASE COUNT          554 a         1349 c         1295 g          728 t
ORIGIN      
        1 gcttcacggt cacgtcgatg ctgtgcggca tcgccacctc gctgaccgag atggtgctgt
       61 tccgcctgct gcagggcgtg ttcggcgccg cgctggtgcc gctgtcgcag gcggtgatgc
      121 tggacatcta cccggccaag cagcgcggct ccgccatggc gatctggggc atgggcgtga
      181 tggtcggccc gatcctgggc cccaccctcg gcggctacct gacggaattc tacaactggc
      241 gctttgtctt cttcgtgaac ctgcccttcg ggctggcggc caccatcggc ctgctgctgt
      301 tcctgccccg cacggagccg caggaatccc gcttcgactg gaccggcttc ctgatgttcg
      361 cgatcggcat cgccgggctg cagatgatgc tggaccgcgg cgagacgaag gactggttcg
      421 gctcgaccga gatcatcgtc gaggcggtga tggccgggct ggggctgtac ctgttcgtgg
      481 tgcacatgtt caccgcgaaa aagccattca tcacgccgcg catcttcaag gaccgcaacc
      541 tgaatgccgg actgctggtg atgttcgcgg tgggcctggt gctgctggcg gtctccgccc
      601 tgctcgcccc gtggctgcag acattgggaa actacccggt ggagaccacc ggcctgatga
      661 tggcgccgcg cggcatcggc accatggcgg cgatgatgat cgccggccgg ctgaccaaca
      721 aggtggatcc ccgcgcgctg atggccttcg gcatcctggt gctgagctgg agcctgtacc
      781 ggatgaccgg ctggactccc gcggtgaacg agaccgagtt cgtggtcaac accatcgtcc
      841 agggcttcgg cctgggcttc gtgttcatcc cgctgcaggt gatcgccttc gccacgctgg
      901 aacccatgct gcgcaccgaa ggcaccgcgc tgctgagcct gacccgcaat gttggcagcg
      961 cggtggggat ctccgtcacc gaggcactgc tgacgcacaa cacgcaaatc gagcatgact
     1021 cgctcagcaa gttcgcctcc ccgctcaacc gtgcgttcca gtcgctgggc ccgatgggcc
     1081 acgcgctggc gcccaccacc tcgcagggac agcagatcct gaacgggatg atcaaccagc
     1141 aggcgcagat cattgcctac aacgacgact acaagctgat gatgctgacc gcgctgccga
     1201 tgctgttcct gctgctgctg atgcggcggc cgcgcggcgg caatgccggc ggcgatcacc
     1261 cggccgtact ggattgacgc cgaagctgcc tcccggccgg cgaacaggca ccgtcacgct
     1321 tgcctgttcg ccggcacggt gacatcaccc cggtcccttc ctcccgccag gcagcatgtc
     1381 cagttcgcgc acagccggca gccgctcctg gagcctggcc gccgccatat ccgccgtcgc
     1441 ggtgttcggg ctgggcgtcg ggcagttcgc gccgctgctg tcgctgctgc tggatgcgcg
     1501 cggcgtaacg ccgacgctga acgggttgaa cgcggccacg tcctccatcg gcatcgtgct
     1561 tggcccgctc ctcgctccgc gcggcgtgcg ggcgctgggg gtcaaccgct tcctgatgct
     1621 gtgcttcgtg ctggacatca tcctcgtcct ggcgctgaag ctgttcgaca ccgtcgccgc
     1681 ctggttcgtg ctgcgcttcc tgctcggact ggtgggctcc aacatcttca cggcgagcga
     1741 ggcctggatc aaccggctgg cgggcgatgc cggccgtggc cgcgtgctgg gcctgtatgc
     1801 cgctctgctg tccgccggct tcggcgtggg accgctgctg ctggtgctga ccggcgtgca
     1861 tggctggtgg ccattcgcga tcaacgcggc gatcaccgcc atcgcgttgc tgccgctgct
     1921 cgccgccggc ggtggcatgc ttgacctcgg ccgcgagcaa tcgggctcca gcattgccgc
     1981 cgcgcggcgg gcgccgatga tcttcgtgac cgttgcgctg ttcggcttgt tcgagttcgc
     2041 actgatggcg ctgttgccgg tctgggcgct gcgccacggc atggatgcgc ggcaggccgc
     2101 cggcacgctc tccggcgtat atatcggcgc gatcgcgctg caggtgccgg tgggatggtt
     2161 gtccgaccgg ctgtcacgcc gcgccgcatt gcggctgtgc ggcacggtcg gtctttcagg
     2221 tgctgccgtg ctgttcgcgg tgccgctgtc cgggccggca cttttcgcgt ttctgttcct
     2281 ctggggcgga accgttgccg cgatctatcc ggtggcgctc agcatggccg gcgaccgctt
     2341 cccacccggc gagatggtcg ccatgaatgc cgcggtgatc agcgcctatg gtatcggctc
     2401 gctgatcggc ccggcgctgg gcggcgccgc gatggacaca tggaattccc aggggctgcc
     2461 tgggatgttc atccttctgt tcgcgctgtt cctgctgctg acattcaccc gcgcgcaccg
     2521 tccgggcacg taggaccgac ggaaggatgg ctgttcacgc ccgcctcccc atctgaagca
     2581 gagcagagaa cccgttggag gcttcgtgcg tctgcccttc cgcatcaccg tctgcggcat
     2641 cgacgaattg tccggtcatt gtgaggccgg tgtcagtcat gtgctgtcga tcctcgatcc
     2701 cggtcatccc gagccgagcg cctttggctc gttcggggaa catgtccggc tggaattgcg
     2761 cttcaatgac atcatcgagg aagcgccgga catgcagatg ccctcgcgcc tgcatgtgga
     2821 acaattgctg gcgttcggcc gcaccttgcc caccgaggcg caggcccacc tgctggtgca
     2881 ttgccatgcg ggaatttcgc ggtcctcggc gtcgatgacg ctgctgctgg cgcaggcgat
     2941 gccggactgt ccggcggcgg cgattttcga tgaagtgctg cgcatccggc cgatcatctg
     3001 gccgaacctg cgcatcctgg agctgggtga tgcggcgttg cgccgggatg gcgcactgat
     3061 cgaggcggct gccggactct acggccgaca gcttgaacgc cgcccggaac tgtcggaact
     3121 gtaccgcggc aacggacgcg gccgcgaagt gcagatgggg cggatggacg gcgtcacgaa
     3181 cggttgatcg tgcgccgacc ggcgatcata ccggcgcgct ccaccgcatc cgccgccttc
     3241 actccgtgca gccctcgtcg cgcagcaggt ccactaccgc atccatcggc acatcggccg
     3301 aggtgaacgc ctggccgatc ccgcgcacca gcacgaagtt cagcgcgccg tcacgcacct
     3361 tcttgtcccg ccgcatgtgc ccgatcaggg tcgcggcgga gaagcggcgg ttcagcatcg
     3421 acagttccgc cggcaacccg gccccggcaa catgcgccac cacccgttcg gcgtccgccg
     3481 aagaacaatg ccccagccga gcagagagct tgaaggcaag accaagcccg accgcgacgc
     3541 cttcaccgtg caacaggccg ccggtgtagc catattcggc ttccagagcg tggccgaagg
     3601 tgtgacccag gttcaacagc gcacggccgt cgttcggctt ttcttcccgc tcgtcatcgc
     3661 caaccaccgc ggccttgaag gcgcaggcgc gtttgatcgc ctcggcctgc gcctcgcgat
     3721 cgccgccgac cacgccggcg ccatgctgct cgcaccagcg gaagaaggcc gggtcgccga
     3781 tcaggccggc tttgacgatt tccgcgtagc cggcccgcaa ttcccgtacc ggcagggtcg
     3841 ccagcgtggc ggtatccgcc agcaccatgc gcggctggtg gaacgcgccc accaggttct
     3901 tgccgcgcgg cgtgttcacc ccggtc
//
LOCUS       DASSZH010000088         5755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_973804, whole genome shotgun sequence.
ACCESSION   DASSZH010000088 DASSZH010000000
VERSION     DASSZH010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5755
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973804"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1763
                     /gene="cydC"
                     /locus_tag="VFN42_01740"
     CDS             135..1763
                     /gene="cydC"
                     /locus_tag="VFN42_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HET8995368.1"
                     /translation="MTDLLRILALWRNRTGWLLFGLVISLVALAAGVGLMVLSGRMIA
                     TAVALGVVLVPAALDAFGITRVAGRYFERLVTHAATFRALADLRVWFFRHLARTGAGG
                     LGFRQAGDVLARLVGDVEALDGLYLRILLPLAGAVLLLPGIAVLIGVHLPGVAVAVAV
                     LFAVSAFILPLIAARSAHAAGAQLAETAGSLRIAALDALTGLREVRAFGAEGRMLATV
                     QAREGALLAAQHDLARRTALAGAGAFLCGQAAVFAILLAAGTAPVPAVVAAFVVVAAF
                     EAVGGLPRAGALAGHAAAAARRVLAVADAPVPVPDPQRPAATPTGFGLRAEGVHFRWQ
                     PDRPAVFDGLTLDIPAGARVALLGPSGAGKSTLSALLLKVAAPQSGRILLGGTDIAQL
                     PADVLHAHIGWLSQSTHLFDDTIRANLLLARPDARDDDLWAALDAAQIGDTVRALPDG
                     LETWVGEGGARFSGGQGRRLALARALLSPAPILILDEPCAGLDADTERDFLTTLNEVA
                     EGRTVILIAHRLIGVEKLDRIWRLSGGKAVAAAA"
     gene            complement(1776..2282)
                     /locus_tag="VFN42_01745"
     CDS             complement(1776..2282)
                     /locus_tag="VFN42_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995369.1"
                     /translation="MPDTPVAPPERPAYDPSLEVRVTRLEDDGRDMRSVVADLSGNVA
                     RLNERVGRLDDRVGHLDERVGRLDDRMGRVEERLGRVEIDVAEIKALLLATLPHLATK
                     ADVAEARAEITTAKAETANVRAEAATFRAELISQLAEKPSKTYMWGVMGALLTAYACG
                     LAALAILK"
     gene            2497..4320
                     /gene="recQ"
                     /locus_tag="VFN42_01750"
     CDS             2497..4320
                     /gene="recQ"
                     /locus_tag="VFN42_01750"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HET8995370.1"
                     /translation="MLQSLLPESPQAVLRRVFGFPGFRGQQEHVVQHVCAGGDALVLM
                     PTGGGKSICYQVPALCRQGTAVVISPLIALMDDQVAALRQVGIAAGALHSDLDPAEAR
                     SVTRDLIEGQLDLLYVSPERLLGNGTLDRLSRVPLTLFAIDEAHCVSQWGHEFRPEYR
                     ALACLPQRFPGVPRVALTATADPRTRADILSALDMQDAAVFVSSFHRPNLRLAAEPKV
                     GETSQLLDFLARHPGECGIVYCGSRAKTERIAARLQGKGMQAIAFHAGLDAETKRAAL
                     TRFRSGEAVVIVATIAFGMGIDRPDVRFVVHLDMPDTPEAWYQQIGRAGRDGDPADTL
                     LLYGGQDIAQARHWLAQSAAPDQQKRVMRTKLEEMIGLTESVTCRTRALLTCFGEEMT
                     AACGHCDNCDMPPITADATVEAQKVLSAVYRTDQIFGAMHIIAVLRGEKTEAVLRHAH
                     DRLPTFGVGAERSVAYWRGLIRQLIALGALDVDTAGHGGLFLVQDKARPILRGETRVL
                     MRQETARRARDVERPARAGAATPEITGESAGLFESLRAWRASQAKAQSVPPYVIFHDT
                     VLREIAAVRPADLEELGQIKGVGASKLQRYGLAVLNVLAAA"
     gene            4570..4839
                     /locus_tag="VFN42_01755"
     CDS             4570..4839
                     /locus_tag="VFN42_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014106103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET8995371.1"
                     /translation="MSKAFLAQVIQDHAELTGAAANRTAGALMDAIVKELKKNGKFTL
                     PSFGTFTVRKTKARKGLNPRTGESIKVKAGKTVRFKASPTLKKAV"
     gene            4940..>5755
                     /locus_tag="VFN42_01760"
     CDS             4940..>5755
                     /locus_tag="VFN42_01760"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8995372.1"
                     /translation="MVCLGTLVVPFDSAVNVAFPDIVRGFGLPIPAIQWVIIAYTLTY
                     AALMLVFGRIGDMLGYRRIFLLGCFWSFIAFLLCAGAPSFAWLLGARVLQGVGAALVL
                     SCGAALVTGLYPESQRTRILGIYTMLFGLGGALGPLVAGLLVAEWGWPAVFWARAPLA
                     LVALALGWRLPAHAAQASQERFDAAGAALLVVAISAFLLTLNQMHDIAHNADRVAIAG
                     VLAVAAGTGFVWRELHTPHPILELRFFRTIDFSLANAAQTILNLAGFAVLLLVP"
BASE COUNT          779 a         2011 c         1974 g          991 t
ORIGIN      
        1 cccctcccct tgcgggaggg ggatgggggg agggggccaa tgagcgtccc cgggacactc
       61 caaccacccc ctccccccaa ccccctcccg caaggggagg gggagttcct tctccgtcgc
      121 gacgttttcg tcccatgacc gacctcctcc gtatcctcgc gctctggcgc aaccgcactg
      181 gctggctgct gttcggcctg gtgatctccc tggtcgcgct ggccgccggc gtcggactga
      241 tggtgttgtc cggccggatg atcgccaccg cggtcgcatt gggcgtggtg ctggtccctg
      301 cggcgctgga cgccttcggc atcacccgcg tcgctggccg ctatttcgag cgcctggtca
      361 cccacgccgc caccttccgc gctttggccg acctgcgcgt gtggttcttc cgccacttgg
      421 cgcgcaccgg cgccggcgga ctgggcttcc gccaggccgg cgacgtgctg gcccggctgg
      481 tcggcgatgt ggaggcgctg gatgggctgt atctgcggat cctgctgccg ctggccggcg
      541 cggtgctgct gctgcccggc atcgcggtgc tgatcggcgt tcacctgccg ggcgttgccg
      601 tcgcggtggc ggtgttgttc gccgtctccg ccttcatcct gccgctgatt gccgcccgca
      661 gcgcccacgc cgccggcgcg caacttgcgg aaaccgcggg cagcctgcgc atcgccgcgc
      721 tggatgcact gaccggactg cgcgaagtgc gcgcgttcgg cgccgaaggg cgcatgctgg
      781 ccaccgtgca ggcgcgggag ggtgcgctgc tcgcggcgca gcacgatctc gctcggcgca
      841 ccgcgctggc cggcgccggc gccttcctct gcggccaagc cgcggtgttc gccatcctgc
      901 tcgccgccgg aacggcgccg gtgcccgcgg tggtcgccgc cttcgtcgtg gtcgccgcct
      961 tcgaggcggt cggcggcctg ccgcgcgccg gcgccctggc cgggcatgcc gctgccgcgg
     1021 cccgccgcgt gctggccgtc gccgacgcgc ccgtgccggt gcccgatccg cagcgcccgg
     1081 ccgcgacccc caccggcttt ggcctgcgcg ccgagggtgt gcatttccgc tggcagccgg
     1141 accgccccgc ggtgttcgat ggcctgaccc tggacatccc cgccggcgcg cgcgtcgcgc
     1201 tgctcggccc ctctggcgcc ggcaagtcca ccttgtccgc cctgttgctg aaggtcgccg
     1261 cgccgcagtc ggggcgcatc ctgttaggcg gcacggatat cgcccagttg ccggccgacg
     1321 tgctgcatgc ccacatcggc tggctcagcc agtccaccca cctgttcgat gacacgatcc
     1381 gcgccaacct gctgctcgcc cggccggacg cgcgggacga cgatctgtgg gccgcgctgg
     1441 atgcggcgca gatcggcgac acggtgcggg cgctgcccga cgggctggag acctgggtgg
     1501 gagaaggcgg cgcccgcttc tccggcggcc aggggcgccg cctggcactg gcgcgcgccc
     1561 tgctgtcgcc tgcccccatc ctgatcctgg acgagccctg tgccggcctg gacgcggata
     1621 ccgagcgcga cttcctcacc acgctgaacg aggtggcgga aggccgcacg gtgatcctga
     1681 ttgcgcaccg gctgatcggc gtggagaagc tggaccggat ctggcggctg tccggcggca
     1741 aggcggtcgc cgccgccgca tagcagcgcg ccggcctatt tcaggatggc cagcgccgcc
     1801 aatccgcagg cataggcggt cagcagcgcg cccatcacgc cccacatgta cgtcttgctc
     1861 ggcttctccg ccaactggct gatcagctcc gcccggaagg tggcagcctc ggcccgcaca
     1921 ttggctgtct cggccttggc ggtagtaatc tcggcccggg cctcggcgac atccgccttg
     1981 gtggcgagat gcggcagggt ggccagcagc agcgccttga tttcggcgac atcaatctcc
     2041 acgcggccga gtcgctcctc gacgcgaccc atacggtcgt caaggcggcc gacgcgttcg
     2101 tccaggtgac caacgcggtc gtccaggcga ccgacgcgct cgttcaaccg ggccacgttc
     2161 ccgctcaggt cggcgaccac agacctcata tcgcgtccgt catcctccag ccgtgtcacc
     2221 cgcacctcca gggagggatc gtaggcaggc cgctccggcg gagcaacagg agtgtcgggc
     2281 atggcggcgc cttcgcttgc tggcgccatt ctggaatcca agggttaaga ttccctgatg
     2341 cccaatcgtt cgcgttgacg gcgccttcat ccatcggcag gagactcagc ggttccagcc
     2401 gcgagggaat cgacatgcgc cgattgcacg gcgtcctgcc actcatggat gttgccgccc
     2461 gccgcgtacg ccgcgttgac gggcgctgag ccggcgatgc tgcaatcgct cctgcccgaa
     2521 tcgccacagg ccgtgctgcg ccgcgtgttc ggctttcccg gcttccgcgg tcagcaggaa
     2581 cacgtggtgc agcatgtctg tgcaggcggc gacgcgctgg tgctgatgcc caccggcggc
     2641 ggcaagagca tctgctacca ggtgcccgcg ctgtgccgcc agggcaccgc cgtggtgatc
     2701 tcgccgctga tcgcgctgat ggacgaccag gtcgccgcgt tgcgccaggt cggcatcgcg
     2761 gccggcgccc tgcactccga tctcgatccc gccgaggcgc gcagcgtcac ccgcgatctg
     2821 atcgaaggcc agctcgatct gctctacgtc tcgccggagc gcctgctggg caacggcacg
     2881 ctggaccggc tgtcgcgcgt gccgctgacg ctgttcgcca tcgatgaggc gcattgcgtc
     2941 tcgcaatggg gtcacgaatt ccgtccggaa taccgtgccc tggcctgcct gccgcagcgc
     3001 ttccccggcg tgccgcgcgt cgcgctgacc gcgacggccg atccacgcac ccgcgccgat
     3061 atcctgtccg cactggacat gcaggatgcc gcggtcttcg tctccagctt ccatcggccg
     3121 aacctgcggc tggccgccga acccaaggtt ggcgagacgt cgcaactgct ggacttcctg
     3181 gcgcggcatc ccggcgaatg tggcatcgtc tactgcggca gcagagccaa gaccgagcgg
     3241 atcgccgcgc ggctgcaagg caagggcatg caggcgatcg ccttccatgc cgggctggac
     3301 gccgagacca agcgtgcggc gctgacccgt ttccgctccg gtgaagcggt ggtgatcgtc
     3361 gcgacgatcg ccttcggcat gggcatcgac cggccggacg tgcgcttcgt ggtgcacctg
     3421 gacatgcccg acacgccgga ggcatggtac cagcagatcg gacgtgccgg acgcgacggc
     3481 gatccggccg acacgctgct gctgtacggc ggccaggaca tcgcacaggc gcgccactgg
     3541 ctggcgcagt ccgccgcgcc ggaccagcag aagcgagtca tgcgcactaa gcttgaggag
     3601 atgatcgggc tgaccgagtc ggtcacctgc cgcacgcgcg cgctgctgac ctgcttcggg
     3661 gaagaaatga ccgccgcgtg cggccattgc gacaattgtg acatgccgcc gatcaccgcc
     3721 gatgcgacgg tggaagcgca gaaggtgctg tctgcggtgt accgcaccga ccagattttc
     3781 ggggccatgc acatcatcgc cgtgctgcgc ggcgagaaga ccgaggcggt gctgcgccat
     3841 gcgcatgacc gcttgccgac cttcggcgtc ggtgcggagc gcagcgtggc gtattggcgc
     3901 ggactgatcc ggcagttgat cgctctcggt gcactggatg tggataccgc aggccatggc
     3961 gggctgttcc tggtgcagga caaggcgcgt ccgatcctgc gcggcgaaac ccgtgtgctg
     4021 atgcggcagg aaaccgcgcg ccgtgcacgc gacgtcgaac gtcccgcgcg tgcgggcgcc
     4081 gccacgccag agataacagg cgaatcggcg ggtctgttcg agtcgctgcg tgcctggcgt
     4141 gcgtcacagg caaaagccca atccgtgccg ccctacgtca tcttccacga cacggtgctg
     4201 cgcgagatcg ccgcggtgcg gcctgcggac cttgaggaac tgggccagat caagggtgtc
     4261 ggtgcgtcca agttgcagcg ctatggcctt gcggtactga acgtgctggc agcagcctag
     4321 cacgcgactg ggctttgcca gctgcccgat tcacccctgc ctggacggtt gggatgccca
     4381 aacgaccatt ttttgtatcg gaaccggcct gcaaacctca tagaagggct ggcggactcc
     4441 cggcgacctc gttaaaaccg ttgcaacgat gcggcgaatc ggacgtgcgc ggtgtcctgt
     4501 ctcatggcgg cacgtgctcc tggttgaagg ccccgcgtcg gcaacgccgc cattgcacaa
     4561 ggagccagca tgtcgaaagc cttcctcgcc caggtcatcc aggaccacgc cgagctcact
     4621 ggagcggcgg ccaaccggac ggcgggcgca ttgatggacg cgatcgtcaa ggagctgaag
     4681 aagaacggca agtttacact gccgagcttt ggcacgttca ctgtgcgcaa gacgaaggcg
     4741 cgcaaagggc tgaacccgcg caccggcgag tcgatcaagg tgaaggcggg caagacggtg
     4801 cggttcaagg cctcgcccac gctgaagaag gcggtctgag ccacacgacc atgactgttg
     4861 cggccagggt tgcgtgaagc gccctggcct ttttcctgtc cggcgcccgg gggcgatttc
     4921 gcccgcggca gggctggtcg tggtttgcct gggcaccctc gtggtgccat tcgactccgc
     4981 cgtgaacgtc gcgtttcccg acattgtccg cgggttcggc ttgccgattc cggcgatcca
     5041 gtgggtgatc atcgcctaca cgctgaccta cgcggccttg atgctggtgt tcggccgcat
     5101 cggcgacatg ctcggctatc gccgcatctt tctgctcggc tgcttctgga gtttcatcgc
     5161 cttcctgctg tgtgccggtg cgccatcctt cgcatggctg ctgggcgcgc gcgtgctgca
     5221 gggcgtcggg gccgcgctgg tgctgagctg cggtgccgcc ctggtcaccg ggctgtaccc
     5281 cgagtcgcag cgcacccgca tcctgggcat ctacaccatg ctgttcggcc tggggggcgc
     5341 gctggggccg ctggtcgctg gcttgctggt ggcagaatgg ggctggccgg ccgtgttctg
     5401 ggcgcgcgca ccgctggccc tggtggccct ggcgttggga tggcgattgc cggcgcacgc
     5461 cgcgcaggca tcgcaggagc gcttcgacgc agccggcgcg gccttgctgg tcgtcgcgat
     5521 cagcgccttt ctgctgacgc tgaaccagat gcacgacatc gcgcacaatg ccgatcgcgt
     5581 ggcgatcgcc ggcgtgctgg cggtcgctgc cggcaccgga tttgtctggc gggagttgca
     5641 cacgccgcac ccgatcctgg agcttcgttt cttccgcacc atcgatttca gcctggctaa
     5701 cgcagcgcag accatattga acctcgccgg cttcgcggtg ctgctgctgg tgcca
//
LOCUS       DASSZH010000089         3261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_650307, whole genome shotgun sequence.
ACCESSION   DASSZH010000089 DASSZH010000000
VERSION     DASSZH010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650307"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..2741
                     /locus_tag="VFN42_01765"
     CDS             105..2741
                     /locus_tag="VFN42_01765"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HET8995373.1"
                     /translation="MATLDSTRPTPEQFLQLIRRQERGRLKVYLGSNAGVGKTYAMLR
                     EGHRLRKQGVDVAIGLIETHGRAETAEQIGDLEVIPPRRIEYRGMTLREMDLDAVLAR
                     RPTVCLVDELAHTNVPGSRHHKRWEDVAELLEAGIHVWSTLNVQHLESLNDAVAQITG
                     VRVSETLPDRVLEMADEIELVDLSPGDLRERLREGRIYQPDVARRALEGFFREGNLAA
                     LRELALRRAAQHVDQNVRDYMRQKAIPGPWPAGDRVLALIGADDRAEAVVRQAKRLAD
                     ALHAPWIALHFERPDALAEPRPALDIAAQLGAEVESRAGSGDLVRTIVELARSRNVTH
                     IVAGRGHPPFWRRMIGRTLIGSLVREGGAFTVHVVANPGGAARPPVRTPRSPATKLPW
                     LIATLCAGVVVAAGELMHPWLHAEALGMMFIAGVVAVATMYGLAVALYSAALGFLCWN
                     ILFIPPVYRFTIYDSRDIVALLVFVGVAGATGLLASRVRAEARAAQSRIEGLRRIAGF
                     TRSLGVPTTETDLCDEIARQAASIAERSMVLIGTGGDVELRSTVPPADMLDEASWAAA
                     RWAYAYGEPAGRGTATLPSCEWRFLPLRTVRGVLGALGMHSQQGLNEPQVQALSTLAD
                     QGAAALERIRFAAEAAHADAQAETQKLRTALLNSLSHDLRTPLTSIRGSAGLLRASWD
                     SLAPAARADLLSSIEHDTVLMTGFLANIMEMTRLESGEIAPQFEKLVARDVVDAALAR
                     MEGGNLLLVNLPEDLPAMRADPQLLETVLVNALENAAKYSPAGSVLRVTGERQGNRVC
                     IAIADEGMGIPADDLPHIFDSFYRVKREDRTRPGTGLGLAIARGLVEAMEGTITVQSP
                     RPDAPKDGAPGTVVTVCLPVAP"
     gene            2738..>3261
                     /locus_tag="VFN42_01770"
     CDS             2738..>3261
                     /locus_tag="VFN42_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8995374.1"
                     /translation="MTAARILVVDDEPQIQRFLRPALQTAGYIVEPAGSGQACLKAIA
                     TRPPDAVLLDLGLPDLDGQEVLRRLRAVSRVPVVVVSARDRVDEKIQALDAGADDYVE
                     KPFDLGELLARLRAALRHGFAQEGAAPEFRNGPLRVDLLRRHIFVDGAAVTLTPREYA
                     LLALLVRHAGRVVT"
BASE COUNT          489 a         1141 c         1155 g          476 t
ORIGIN      
        1 ccggggcgat cctcggcgcg ccgccccgcg tcaacgtgct ccggctgaac ctgaccctgg
       61 acgaggagcc ggctccgcgc gaccacgaga gggactagac cgcgatggcg accctcgatt
      121 cgacccgacc cacgcccgag cagttcctcc agctcatccg ccgccaggag cgcgggcggc
      181 tgaaggtcta cctcggctcg aatgccggcg tgggcaagac ctacgccatg ctccgcgagg
      241 gccaccgcct gaggaagcag ggcgtggacg tggccatcgg cctgatcgag acccacggcc
      301 gggccgagac ggccgagcag atcggcgacc tggaggtcat cccgccccgg cggatcgagt
      361 accggggcat gacgttgcgc gagatggacc tcgatgccgt cctcgcccgc cgccccaccg
      421 tctgcctggt cgacgagctg gcgcacacca acgtccccgg cagccggcat cacaagcgct
      481 gggaagacgt ggcggagctg ctggaggccg gcatccatgt ctggtccacg ctgaacgtcc
      541 agcacctgga aagcctgaac gacgccgtcg cgcagatcac cggcgtacgg gtcagcgaga
      601 cgctgcccga ccgtgtgctg gagatggccg acgagatcga attggtcgat ctcagccccg
      661 gcgacctgcg ggagcggctg cgtgagggcc gcatctatca gccggatgtc gcccggcgtg
      721 ccctggaagg cttcttccgc gagggcaacc tggccgcctt gcgcgagctg gcgctgcgcc
      781 gggcagccca gcatgtggac cagaacgtgc gggactacat gcggcagaag gcgatcccgg
      841 gtccttggcc ggccggcgac cgtgtcctgg cgctgatcgg cgcggacgac cgcgccgagg
      901 ccgtggttcg ccaggcaaag cgactggccg atgcgctgca cgccccctgg atcgcgctgc
      961 atttcgagcg gccggacgcc ctggccgaac cccgcccggc cctggacatc gccgcgcagc
     1021 tcggcgccga agtggaaagc cgcgccggca gcggcgacct ggtccgcacg atcgtcgaac
     1081 tggcacgctc ccgcaacgtc acccacatcg tcgcgggacg cgggcacccg ccgttctggc
     1141 gccgtatgat cggccgcacg ctgatcggca gcctggtgcg cgagggcggc gcgttcaccg
     1201 tgcacgtggt agccaatccg ggcggagccg ctcgcccgcc ggtgcgcacc ccacgatcgc
     1261 ccgccaccaa gctgccttgg ctgatcgcca cgctgtgcgc cggcgtcgtg gtcgccgccg
     1321 gcgagctgat gcatccctgg ctgcatgccg aagcgctggg catgatgttc atcgccgggg
     1381 tggttgctgt cgccacgatg tacggcctgg cggtggcgct gtattcggcc gcacttgggt
     1441 tcctgtgctg gaacatcctg ttcatcccgc cggtgtaccg cttcaccatc tacgattcgc
     1501 gcgacatcgt cgccctgctg gtgttcgtcg gcgttgccgg cgccaccggc ctgctggcca
     1561 gccgggtccg cgccgaggcg cgcgccgcgc agagccgcat tgaaggattg cggcgcatcg
     1621 ccggtttcac ccgcagcctg ggcgtgccga ccaccgaaac cgacctgtgc gacgaaatcg
     1681 cgcggcaggc cgccagcatc gccgaacgct cgatggtgct gatcggcacc ggcggcgacg
     1741 tcgaattgcg ctctaccgta ccgccggccg acatgctgga cgaagcgagc tgggccgcgg
     1801 cgcgatgggc ctatgcctat ggcgaaccgg ccggccgcgg cacggccacg ctgccatcct
     1861 gcgagtggcg cttcctgccg ctacgcaccg tgcgcggcgt gctgggcgcg ctgggcatgc
     1921 actcccagca aggactgaac gaaccgcagg tccaggcgct gagcacgctc gccgatcaag
     1981 gcgccgccgc gctggaacgc atccgcttcg cggccgaagc cgcacatgcc gacgcacagg
     2041 cggaaaccca gaagctgcgc accgccctgc tgaactcact gtcgcacgat ctgcgcacgc
     2101 cgctgaccag catccgcggc tccgccgggc tgctgcgtgc gtcctgggac agcctggcgc
     2161 cggcagcgcg cgccgacctg ctgtccagca tcgagcacga cacggtgctg atgaccggct
     2221 tcctggccaa catcatggag atgactcggc tggagagcgg cgagatcgct ccccaattcg
     2281 aaaagctggt ggcgcgcgat gttgtggacg ctgctctggc ccggatggaa ggcgggaacc
     2341 tgctgctggt caacctgccg gaggacctgc cggcgatgcg ggccgatccg cagctactgg
     2401 agaccgtgct ggtgaacgcc ctggagaacg cggcgaagta cagcccggcg ggatcggtgc
     2461 tgcgtgtcac cggcgagcgc cagggcaatc gcgtctgcat cgccatcgcc gatgagggca
     2521 tgggcatacc ggccgacgat ctgccgcaca tcttcgacag tttctaccgg gtgaagcgtg
     2581 aggaccgcac gcggcccggc acgggattgg gcctggccat cgcgcgtggc ctggtggaag
     2641 cgatggaggg caccatcacg gtgcaaagcc cgcggccgga cgcgccgaaa gatggcgcgc
     2701 cgggcaccgt ggtgacggtc tgcctgcccg tcgcgccgtg accgctgccc gcatcctggt
     2761 ggtggatgac gaaccgcaga tccagcgttt cctgcgcccg gccttgcaaa cggcgggcta
     2821 catcgtggag ccggccggca gcggccaggc ctgcctgaag gcgatcgcga cgcggccgcc
     2881 ggatgcggtg ctgctggacc ttggcctgcc ggatctcgac ggtcaggaag tgctgcggcg
     2941 cctgcgcgcc gtgtcacgtg ttccggtggt ggtggtctcc gcgcgcgacc gggtggacga
     3001 gaagatccag gcgctggatg ccggcgccga cgactacgtg gaaaagccgt tcgatctggg
     3061 ggaattgctg gcacggctgc gcgccgcgtt gcggcatggc ttcgcgcagg agggcgccgc
     3121 accggaattc cgcaacggcc cgctgcgcgt cgatctgctg cgccgccaca tcttcgtcga
     3181 tggcgcggcg gtgacgttga cgccgcggga atatgcgctg ctcgcgctgc tggtgcgcca
     3241 tgccgggcgc gtggtgacgc a
//
LOCUS       DASSZH010000090         2197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1178419, whole genome shotgun sequence.
ACCESSION   DASSZH010000090 DASSZH010000000
VERSION     DASSZH010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178419"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..1633
                     /gene="radA"
                     /locus_tag="VFN42_01775"
     CDS             224..1633
                     /gene="radA"
                     /locus_tag="VFN42_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019084935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET8995375.1"
                     /translation="MARSSPRFVCQSCGAVFPKWAGRCETCGEWNTIIEEAAPAARPG
                     PAGKAPVGRAVSFVGLGGTAEPPPRAITGIAELDRVLGGGLVPSSAVLVGGDPGIGKS
                     TLLLQAAAQLAQSGKRVLYVTGEESIEQVRLRARRLGIADTPIELAAAINLRDIAATL
                     EQTRDAALVVIDSIQTMWLDSIDSAPGTVGQVRASSFELIRLAKGGNFALVLVGHVTK
                     DGALAGPRVLEHMVDAVLYFEGDRGHQFRILRAVKNRFGATDEIGVFEMTDRGLAEVA
                     NPSALFLAERRGNIAGSAVFAGLEGTRPVLMEIQALLSPNAGGSPRRSVIGWDSGRLS
                     MLLAVLETRAGLRLNAMDVYLNVAGGLRVTEPAADLAVAAALASAACNHPTSAGMVFF
                     GEVGLSGEVRQVAQAEARLKEAAKLGFDAATLPRRLARGNRAVAAPEGLRLEEIGHIA
                     DLVARFAPEAIQEAEEGDR"
     gene            complement(1644..1817)
                     /locus_tag="VFN42_01780"
     CDS             complement(1644..1817)
                     /locus_tag="VFN42_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995376.1"
                     /translation="MSVIAIETDLHGATRVFFDYRAAPADGTDERRIERAIVQALAAD
                     RLLSRDHAIEPTR"
     gene            complement(1833..2096)
                     /locus_tag="VFN42_01785"
     CDS             complement(1833..2096)
                     /locus_tag="VFN42_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995377.1"
                     /translation="MNMQLIGAGDKAMAGVNCPETALRRMSAEQLLQLGVDQVAYVRT
                     SRHDGVPYFLIHGADGVLLTTVDRLEQVMEIAAELGFEFVTVH"
BASE COUNT          350 a          725 c          759 g          363 t
ORIGIN      
        1 aactaccgga tgatgcggcc tgccgcggaa aagcctacca gatgttgcag atcggactca
       61 gtgaccgccg ctaatcagcg ctatcgcgca gaaggcccag atgcgagcga attcgcattt
      121 agaaccgctg attgccgccc cgtgctggcg actccggtct ccgcgctaag caacgggtaa
      181 tgcgggccgg gcatgctggc cgcatgtttg cggagaatgt caggtggcca ggtccagccc
      241 ccgtttcgtc tgccagtcct gcggtgccgt gttcccgaaa tgggccgggc gctgcgagac
      301 ctgcggcgag tggaacacca tcatcgagga agccgccccc gccgcccgtc ctggcccggc
      361 gggcaaggcc cccgtcggcc gggcggtaag cttcgtcggc ctgggtggca ccgccgaacc
      421 gccgccgcgc gcgatcaccg gcatcgcgga gctggaccgg gtgctgggcg gcgggctggt
      481 gccgtcctcc gcggtgctgg tcggcggcga tccgggcatt ggcaagtcca ccctgctgct
      541 gcaggccgcc gcgcaactgg cgcaatccgg caagcgcgtg ctgtacgtga cgggcgagga
      601 atccattgag caggtccgcc tgcgcgcccg ccgcctgggc attgccgaca cgccgatcga
      661 actggccgcc gccatcaacc tgcgcgacat cgccgccacg ctggagcaga ccagggacgc
      721 cgcgctggtg gtgatcgatt cgatccagac catgtggctg gacagcatcg acagcgcgcc
      781 gggcacggtc ggccaggtgc gggcgagcag ctttgagctg atccggctgg caaaaggcgg
      841 aaacttcgcg ctggtgctgg tcggccatgt caccaaggac ggcgcgctgg ccggcccgcg
      901 cgtgctggaa cacatggtcg atgcggtgct gtatttcgaa ggcgaccgcg gccaccagtt
      961 ccgcattctt cgagcggtga aaaaccgctt cggtgcgacc gacgagatcg gcgtgttcga
     1021 gatgaccgat cgcggtctgg ccgaagtggc caatccctcc gcgctgttcc tcgccgaacg
     1081 ccgcggcaac atcgccggca gcgcggtgtt cgccgggctg gaaggcacgc ggccggtgct
     1141 gatggagatc caggcgctgc tgtcacccaa tgccggcggt tcgccgcgcc gctcggtgat
     1201 cggctgggac agcgggcggc tgtccatgct gctggcggtg ctggaaaccc gcgccgggct
     1261 gcggctgaac gcgatggacg tctacctgaa cgtggccggt gggctacggg tgacggagcc
     1321 ggcggccgac ctggccgtgg cggcggcatt ggcttctgcc gcgtgcaacc atccgaccag
     1381 cgccggcatg gtgttcttcg gtgaggtcgg cctgtccggc gaagtgcgcc aggtcgccca
     1441 ggccgaagcc cggctgaagg aggcagccaa gctcggcttt gacgcggcga cgctgccgcg
     1501 ccggctggcg cgcggcaatc gggcggtagc ggcaccggag gggctgcggc tggaggagat
     1561 cggccacatc gccgacctgg tggcccgctt cgcgccagaa gcgattcaag aggcggagga
     1621 aggtgatcgt tgacccgaca aggtcaccga gtcggttcga ttgcgtgatc tcggctcaac
     1681 aggcgatccg cggccagcgc ctgcacgatg gctcgttcga tccgccgttc atccgtgcca
     1741 tccgccggcg cagcgcggta atcgaagaac acccgcgttg ccccatgcag gtcggtctcg
     1801 atcgcaatca ctgacacgca acgccccttc cgtcagtgca cggtgacgaa ttcaaaaccc
     1861 agttcggcgg cgatttccat cacctgctca agcctgtcga ccgttgtgag cagaacccca
     1921 tccgcgccat ggatcaggaa gtagggcacg ccatcgtgcc gactggtgcg cacataggcc
     1981 acctggtcaa cgccgagttg cagcagttgc tccgcgctca tcctgcgcag tgccgtctcc
     2041 ggacagttta cgccggccat tgctttgtcg cctgcgccga tcagttgcat gttcatggtg
     2101 ctggtccttg gatcgggtca taccgccgct gagcacagtg gcggcggggc ttgctgggac
     2161 aaagcaagca ttgccgctgc ctcgcccgca attcgct
//
LOCUS       DASSZH010000091         3687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_241132, whole genome shotgun sequence.
ACCESSION   DASSZH010000091 DASSZH010000000
VERSION     DASSZH010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241132"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /gene="crtI"
                     /locus_tag="VFN42_01790"
     CDS             <1..849
                     /gene="crtI"
                     /locus_tag="VFN42_01790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HET8995378.1"
                     /translation="WGVHFAMGGTGAVVRGLAGLVASQGNIIRCNATVDQILVSNGKA
                     HGVRLASGETMAADIVVSNADSAWTYRKLLPPEARRRWTDRKLDRARYSMSLFLWYFG
                     TSRRYEAVPHHTIMLGPRYRGLLRDIFGRKVLSEDFSLYLHRPTATDPSLAPPGCDAF
                     YVLSPVPNLAGATDWRTAAEPYRQAIAAHLSATVLPDLERHVVSSKLMTPLDFQDQLL
                     AWQGAAFGLEPVLLQSAWFRPHNRSEDIANLYLVGAGTHPGAGVPGVLSSAKVLDRVV
                     PHASVL"
     gene            833..1867
                     /locus_tag="VFN42_01795"
     CDS             833..1867
                     /locus_tag="VFN42_01795"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HET8995379.1"
                     /translation="MPLSSDRDASDQEACRSALRGGSKSFDAAAKLLPRAVRAPAVAL
                     YAFCREADDAIDSGTGSLEWLQQRLHLAYEGQPLAIPADRGLAWIVRDFAIPRALPEA
                     LLEGFAWDATGRRYHTLSDLRAYAVRVAGTVGAMMAVLMGVRDAGRMAAAVDLGVAMQ
                     LSNIARDVGEDARNGRLYLPLDWLHAAGIDTDAFLANPGFSPALAGVIARLLDAADLH
                     YRQAGAGITRLPLACRPGIGAARLLYAEIGREVARRGMDSVSGRAVVPGRRKARVLAA
                     GLPRMILPLQPLAGACVPEGAFLVDAVVQAAPRPAQAGAAIPWWALAERVRWTIDLFE
                     QMERREWMPR"
     gene            complement(2012..3583)
                     /locus_tag="VFN42_01800"
     CDS             complement(2012..3583)
                     /locus_tag="VFN42_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET8995380.1"
                     /translation="MVDGQGRRAARPSAGWRMIVGLLGIVALGAAAPDQSNGDLVAKG
                     KYLADLGDCASCHTAVNGAKFAGGRYMPTPFGPISTPNITPDKATGIGNYTDDQFVRV
                     FHDGINAKSEYLYPVMPYPWFTKVSRNDILAIKAYLFSLKPVDAPRKPNHLTFPFNIR
                     AGLAVWDAMFLKHGLFKPDPTKSAQVNRGAYIVETLGHCGECHNGRNLLGDTRMAAAL
                     RGGPIQNWYAPNITSDVHDGIGKYSDQQLFQFLKTGVSPGMGVAAGPMAETIHDSLKK
                     MTDDDIHAIVAYLKSTPAKSSYQPTERTAYTGPNPAGRETYLNYCASCHQLDGKGLPG
                     AVPSLAGNGAVMAKGPEDVMRVVLGGIEAQGDLAPMPAVGAGMTDQQIADVVNYIRQS
                     WGNKAPPTAGAQYVAKLRPKTFTAMNIGPDGHCPTVVQPQLAPVINDPKSGITAALHA
                     MTNENVLQTAQSLVAKLRSAAPGAKRSDIVNSLTIAYCPVIESDNALSGAQKVATLDN
                     FSERVYSELESHEKE"
BASE COUNT          553 a         1187 c         1261 g          686 t
ORIGIN      
        1 tggggtgtgc acttcgccat gggcggcacc ggcgcggtcg tgcgtggtct ggcgggcctg
       61 gttgcgtccc agggcaacat catccgctgc aacgcgacgg tggaccagat cctggtcagc
      121 aacggcaagg cgcatggggt gcggctggcg tccggcgaga ctatggccgc ggacatcgtc
      181 gtttccaacg cggactcggc ctggacatac cgcaagctgc tgccgccgga ggcacgccgc
      241 cgctggaccg atcgcaagct ggaccgggcc cggtattcca tgagcctgtt cctttggtac
      301 ttcggcacca gccggcgcta cgaggctgtc ccccatcaca cgatcatgct gggcccgcgc
      361 tatcgcggcc tgctgcggga cattttcggt cgcaaggtgc tgtcggagga tttctcgctg
      421 tatctgcatc gtccgaccgc gaccgatcca tccctggcgc cgcccggctg tgacgcgttc
      481 tacgtgctgt cgcccgtgcc caacctggcc ggcgcaacgg actggcgcac cgccgccgaa
      541 ccctatcgtc aggcgatcgc cgcgcatctg tcggcgacgg tgctgccgga cctggaacgg
      601 cacgtcgtca gctcgaaact catgacgccg ctggacttcc aggaccagct gctggcctgg
      661 cagggcgccg ccttcgggct ggagcctgtg ttgctgcaaa gcgcgtggtt ccgcccgcat
      721 aatcgcagcg aggatatcgc caacctctat ctggtcggcg ccggcacgca tccgggggcc
      781 ggggtgccgg gggttttgtc gtcagccaaa gtactggatc gggtggtgcc gcatgcctct
      841 gtcctctgac cgcgacgcca gcgaccagga ggcctgccgc tccgcgctgc gcggcgggtc
      901 gaaaagcttc gacgcggcag cgaagctgct gcctcgcgcg gtccgcgcgc cggcggtggc
      961 gctgtatgcg ttctgccggg aggccgacga cgcgatcgac agcggcaccg gcagcctgga
     1021 atggctgcag cagcgattgc acctggccta tgagggccag ccgctcgcca tccccgccga
     1081 tcgcggactg gcctggatcg tccgggattt cgccatcccg cgcgcgttgc ccgaagcgtt
     1141 gctggagggg ttcgcctggg atgccacggg acggcgttac cacacgctgt ccgacctccg
     1201 cgcctatgcc gtgcgggtgg ccgggacggt cggcgcgatg atggcggtgc tgatgggtgt
     1261 gcgtgacgcc ggccggatgg ccgcggcggt cgatttgggg gtggcgatgc agctgtccaa
     1321 tatcgctcgc gatgtcggcg aggatgcgcg caatggccgg ctgtacctgc cgctggactg
     1381 gctgcacgcc gccgggatcg atacggatgc gttcctggct aatcccggct tttcccccgc
     1441 cctggccggg gtgattgctc gtctgctgga cgcggcggac ctgcattatc gtcaggccgg
     1501 tgcgggcatc acccgcctgc cgctggcctg ccggccgggc atcggcgcag cccgcctgct
     1561 ttacgccgaa atcggccgcg aagttgctcg gcgtggcatg gactcggtgt ccggccgagc
     1621 ggtggtgccc ggccggcgga aggcgcgggt gctggcggcg gggctgccgc ggatgatcct
     1681 gccgctgcaa cccctggccg gtgcctgcgt accggaaggc gcatttctgg tggatgcggt
     1741 ggtgcaggca gcaccgaggc cggcccaggc aggcgccgcc ataccgtggt gggcactggc
     1801 ggagcgggtc cgctggacca tcgacctgtt cgagcagatg gaacggcggg agtggatgcc
     1861 gcgatgaagt ggcccagcgg cgtccaatgg gagtgggcgc tgggcgactt cgtgctgctc
     1921 gccttcctgt tctgggaact ccacaagctc cgccggtccc aacggcggga ccgggagagg
     1981 gtgcagcagg acaaagcgcc ggcggatacg gctactcctt ctcgtgactc tcaagctccg
     2041 aataaacccg ctcgctgaag ttatcgagcg tcgccacctt ctgggccccg gacagggcgt
     2101 tgtcgctctc gatcaccgga cagtaggcga tcgtcaggct gttgacgata tccgaccgtt
     2161 tcgcacccgg tgcggccgat ctcagtttcg cgaccaggct ctgcgccgtc tgcagcacgt
     2221 tctcattggt catcgcgtgc agggcagcgg tgatgccgct ctttgggtca ttgataacgg
     2281 gtgccagttg cggctgcacg acggtcgggc agtgaccatc cggcccgatg ttcatcgcgg
     2341 tgaaggtttt cggccgcagc ttcgccacgt attgcgcccc ggcggtgggc ggcgccttgt
     2401 tgccccagga ttggcggatg tagttcacca cgtccgcgat ctgctgatcc gtcatgccgg
     2461 cacccaccgc cggcatgggt gcaagatcac cctgcgcctc gatcccgccc aggacgacgc
     2521 gcattacatc ctccggtccc ttcgccatga ccgcgccatt gccggccagg gatggcaccg
     2581 cgccgggaag gcccttgccg tcaagctgat ggcaggatgc gcaatagttc aggtaagtct
     2641 cccggcccgc cgggttcggc ccggtgtagg cggtacgttc cgtcggttga tagctggact
     2701 tggccggggt cgatttcagg taggcaacga tggcgtggat gtcgtcatcg gtcattttct
     2761 tcaggctgtc gtggatcgtc tctgccatcg gaccggcggc gacacccatg cccggcgata
     2821 cgccggtctt gagaaactgg aacagttgct ggtcggagta tttgccgatt ccgtcatgca
     2881 cgtcgctggt gatgttcggt gcataccagt tctggatcgg cccgccgcgc agcgcggcgg
     2941 ccatgcgcgt atcgcccaac aggttgcgtc cgttatgaca ttcgccacag tgtcccagcg
     3001 tctcgacaat gtaggcgccg cggttcacct gcgcggactt cgtcggatcg ggcttgaaca
     3061 gaccgtgctt caggaacatg gcgtcccata ccgccaggcc ggcacggatg ttgaatggaa
     3121 aggtcaggtg atttggttta cgcggcgcat cgaccggctt cagcgagaac agatacgcct
     3181 tgatcgccag gatgtcgttc cgagacacct tggtgaacca gggatagggc atgaccggat
     3241 agaggtattc gctcttggca ttgatgccgt cgtggaaaac gcgaacaaac tggtcatcgg
     3301 tatagttgcc gatgccggtt gccttgtcgg gcgtgatgtt gggggtggag atcggaccga
     3361 acggagtcgg catgtagcgc ccgccagcga atttcgcgcc attcacagcg gtatggcagg
     3421 atgcgcagtc gccaagatcg gccaggtact tgcccttcgc gaccagatcg ccgttcgatt
     3481 gatccggcgc cgccgcacca agcgccacga tgccaagcag accgacgatc atgcgccagc
     3541 ccgcggatgg ccgcgccgcg cgccgcccct gtccgtccac catcagatgc tccgccctgt
     3601 tcgaaccgtt tcaagctgaa atcgaacctg acaaccggaa cggcgctctc cgggccgggt
     3661 tgccggccga tgccgcagtt cacgcgg
//
LOCUS       DASSZH010000092         3267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_718542, whole genome shotgun sequence.
ACCESSION   DASSZH010000092 DASSZH010000000
VERSION     DASSZH010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718542"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="VFN42_01805"
     CDS             complement(<1..1134)
                     /locus_tag="VFN42_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8995381.1"
                     /translation="MLTALSIRDVVLIERLDLAFGPGLTVLTGETGAGKSILLDSLGL
                     ALGARAEAGLVRPGAEQASVTACFAPPAGHPVLALLAEQGLEAEDDIVLRRLVAKDGR
                     SRAFLNDQPVGVALLRRAGALLVEVQGQHEQMGLADPASHAALLDAFGVPPELRHAVT
                     RAWRDWREALAALDAAREAIAAAQRDEEWLRHAVHELAALAPQDGEEDHLAAERQRLQ
                     QGERRAEAIAAALAELTPRDRRSPGPAAALRAASRALQRLIPPGQPDAVNPAAPALGA
                     LERAEEALAEAETLLTRLSAEADADPRLLEQAEERLFTLRGAARKHAVAVAELPGLLD
                     SLSARLAALETGEAAIATLEQTAAQACVAYVSAAEALAAARRNA"
     gene            complement(1154..2020)
                     /locus_tag="VFN42_01810"
     CDS             complement(1154..2020)
                     /locus_tag="VFN42_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014104811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HET8995382.1"
                     /translation="MNPNRRPDRAAFRRFGLMAVALLPLLAGCGGSKDDEAKTNAPPP
                     SVDALYNNGIDALNSGRYDTAADQFQSLEQNYPYSTWSVKAQLMQGYTQYKQNHYTDA
                     IGTLNRYIQLHPAGGDVAYAYYLRALCYYEQISDVQRDQQDTREAMAALQEVINRFPG
                     TAYARDAQLKIDLCRDHLAGKEMAVGRWYQEQHLYEAAIGRFQTVLNDYQTTNHVAEA
                     LARLTEIYLVLGLPDEAKRTAAVLGYNYPGSIWYEDIYRQLARDHLLAKGAPVPSGGG
                     GGFFSHAFDWIF"
     gene            2156..>3267
                     /locus_tag="VFN42_01815"
     CDS             2156..>3267
                     /locus_tag="VFN42_01815"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HET8995383.1"
                     /translation="MGRRTSRRFLLIAAPIAVLTVRARAQPAPDVRPVGGTVVAGAAT
                     IARSPTQTSITQTTQRAAINWQGFDVGSRQNVTFAQPSATAMTLNRVTGPNPSQIAGR
                     ITANGQIVLVNPAGVVFYKGAQVNTNGLLVTAAGISDRNFMAGRMVFDQPAQPNAKVE
                     NRGEITIRQAGLAALVAPQVANSGVISARLGHVVLAGARTATLDLYGDGLVAIDVTGQ
                     VRQVPLGPDGKPVTALVTNTGIVRADGGTVQLTAQAADGIVQTLVEAGGRIQADSAGS
                     RTGTIVLSGLGGAIRISGQLDARGRAPDSAGGRIVADTNGDVLVGSSARIDASGSTGG
                     GMIVLGTTLARAASGPAVASTLTARRTRIDAGAKIAA"
BASE COUNT          487 a         1139 c         1145 g          496 t
ORIGIN      
        1 ggcgttgcgc cgagcggctg ctagtgcttc ggccgcgctc acataggcga cgcaggcctg
       61 tgcggcggtc tgctccagcg tggcgattgc cgcctctccg gtttccagcg ccgccagccg
      121 cgcggacaac gagtccagca ggcccggcag ctccgccacc gccaccgcgt gcttgcgggc
      181 ggcgccgcgc aaggtgaaca gccgctcctc tgcctgttcc agcagccgcg ggtcggcatc
      241 ggcctcggcg gagagccggg tcagaagggt ttccgcttcc gccagggcct cctccgcacg
      301 ctccagcgca cccaaggccg gcgcggcggg gttcacggcg tccggttgcc cgggtgggat
      361 cagccgctgc agggcgcggg atgccgcccg cagcgccgcc gccgggcctg ggctgcggcg
      421 gtcgcgcggc gtcaactctg ccagcgcggc agcgatggct tcggcacggc gttcgccctg
      481 ctgcaggcgc tggcgttcgg ccgccagatg gtcctcttcg ccatcctgcg gcgccagcgc
      541 ggccaattca tgcaccgcat ggcgcagcca ttcctcgtcc cgttgcgcgg cggcgatcgc
      601 ctcacgcgca gcgtccagcg cggccagggc ctcccgccag tcccgccagg cacgggtcac
      661 cgcgtgacgc agttcgggcg gcacgccgaa ggcatccagc agcgccgcgt ggctggccgg
      721 atcggccagt cccatctgct cgtgctgtcc ctgcacctcc accagcaggg ccccggcccg
      781 tcgcagcagc gccacgccca ccggctggtc gttcaggaag gcgcgggagc ggccgtcctt
      841 cgccaccagg cggcggagca cgatgtcgtc ctccgcttcc agcccctgtt cggcgagcag
      901 ggccagcacc ggatggccgg cggggggcgc gaagcaggcg gtgacgctgg cctgttccgc
      961 accgggccgc accagtccgg cctccgcccg cgccccgagt gccaggccca ggctgtccag
     1021 caggatggat ttgccggcac cggtttcgcc ggtcagcacg gtcaggccgg ggccgaacgc
     1081 caggtccagc cgctcgatca gcaccacgtc ccggatcgac agcgcggtaa gcatgcggcc
     1141 cgcgcgcgct ggatcagaag atccagtcga aggcgtggga gaagaacccg ccgccgccac
     1201 cgctcggcac cggcgccccc ttggccagca ggtggtcgcg ggcgagctgg cgatagatat
     1261 cctcgtacca gatgctgccc ggatagttgt agccaagcac cgccgcggtc cgcttggcct
     1321 catccggcag gccgaggacc aggtagatct cggtgagccg agcgagggcc tcggcgacgt
     1381 ggttggtggt ctggtagtcg ttcaacacgg tctggaagcg gccgatcgcc gcctcataca
     1441 gatgctgttc ctggtaccag cggccgaccg ccatttcctt gccggccaga tggtcgcggc
     1501 acaggtcgat cttgagctgg gcgtcccgcg cataggcggt gccggggaag cggttgatga
     1561 cctcctgtag cgccgccatg gcctcccgcg tgtcctgctg gtcgcgttga acgtcggaga
     1621 tctgctcgta gtagcaaagc gcgcgcaggt aataggcata ggcgacgtcg ccgccggccg
     1681 gatgcagctg gatgtagcgg ttcagcgtgc cgatggcgtc cgtgtagtgg ttctgcttgt
     1741 attgggtgta gccctgcatc aactgggcct tcaccgacca ggttgaatac gggtaattct
     1801 gttccagcga ttggaactga tcggcggcgg tgtcatagcg gccgctgttc agcgcatcga
     1861 tgccgttgtt gtacaacgcg tccaccgatg gaggcggcgc gttggtcttc gcctcgtcgt
     1921 ccttggagcc gccgcagcca gccagcagcg gcagcaatgc cacggccatc aggccgaagc
     1981 ggcggaaggc ggcacggtcg ggtctgcgat tcgggttcat cggccgggct tatagcatgc
     2041 ggcgaagcgg cgcgaagggc cgcctctcgg acaggcaatc cggcaggtct caattttgtg
     2101 aacgccgggg ctgttccgtc cgggtaactt gcccgctggc aaattcgtga gcgccatggg
     2161 tcgccgtacg tcccgccgct tcctgctgat cgccgccccg atcgcggtcc tcacggtgcg
     2221 cgcccgggcg cagccagcgc cggatgtccg gcctgtcggt ggaaccgtcg tcgcaggtgc
     2281 cgccacgatc gcccgctccc cgacacagac cagcatcacc cagaccaccc agcgggctgc
     2341 catcaactgg caaggcttcg atgtcggcag ccggcaaaac gtgactttcg cccagccctc
     2401 ggcaacggca atgacgctga accgggtcac cgggccgaac ccgtcccaga tcgccggacg
     2461 catcaccgcc aacgggcaga tcgtgctggt gaacccggcc ggcgtggttt tctacaaggg
     2521 tgcccaggtc aacaccaacg ggctgctggt caccgcggcc ggcatcagcg accggaactt
     2581 catggccggc cgcatggtgt tcgatcagcc ggcgcagccc aatgccaagg tggagaaccg
     2641 gggtgagatc accatccgcc aggcagggct cgcggcgctg gtcgcccccc aggtggcgaa
     2701 ttcgggcgtg atcagcgcga ggcttggcca cgtggtgctg gccggcgcac ggaccgcgac
     2761 gctggacctg tatggtgacg gcctcgtcgc gatcgatgtg acgggccagg tacggcaggt
     2821 gccactcggg ccggacggca agccggtgac cgcgctggtg accaacacgg gaatcgtgcg
     2881 cgccgacggc ggcaccgtcc aattgaccgc gcaggccgca gacggaatcg tgcagaccct
     2941 ggtggaggcc ggtggccgga tccaggctga ctcggccggc agccgcacgg gaacgatcgt
     3001 cctgtccggg ctgggcggcg cgattcgcat cagcggccaa ctggatgcgc gaggcagagc
     3061 acccgacagt gccggagggc gcatcgtcgc cgacaccaac ggtgatgtcc tggtcggaag
     3121 cagtgcccgc atcgacgcgt ccggcagcac cggtggcggc atgatcgtgc tcggcaccac
     3181 attggcgcgc gcggcatccg gcccggcggt cgcgtcaacg ctgaccgcgc ggcgaacacg
     3241 catcgatgcc ggtgcgaaaa tcgccgc
//
LOCUS       DASSZH010000093         2006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1076147, whole genome shotgun sequence.
ACCESSION   DASSZH010000093 DASSZH010000000
VERSION     DASSZH010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076147"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /locus_tag="VFN42_01820"
     CDS             complement(<1..1211)
                     /locus_tag="VFN42_01820"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HET8995384.1"
                     /translation="MADHPGLSPEQAVDRLAALHGEAVAALRAALARFVADGTPPDAG
                     TRARFRYPELRLTWRPAGPAPFTRRAWAKLQVPGTYATTITQPAAFRRYLLQQLHPLV
                     EEFGASLAVAPSAQEIPYPYVLENGDELFQGQVTPGELARHFPTPILSAVGDEIADGL
                     WATPEGAPLPLALFDAVRVDYSLRRLLHYTGTDWRAVQPWILLTNYQRYVDQFVRWGI
                     DQLQHPDSPYERLVLPGGGVVRRGEARAEAERRAQEAPWDRFQMPAYHLCRGGDEEGI
                     SIVNIGVGPANAKNITDHLAVLRPHCWLMIGHCGGLRQSQTIGDYVLAHGYLRRDKIL
                     DELVPPEVPVPALAEVQVALQQAAAHVTGEHGEALKRRLRTGTVVTYDDRNWELRWSQ
                     ERRRINLSRAIA"
     gene            1288..1914
                     /locus_tag="VFN42_01825"
     CDS             1288..1914
                     /locus_tag="VFN42_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HET8995385.1"
                     /translation="MAIELWAYDTPNGRKISIALEEMGLPYTVRVVDITKGEQHRPEF
                     LAISPNNRIPAIIDPEGPDGAPISVFESGAVLLYLAQKTRKFWPAGLRAQVPVLEWLI
                     WQVGGFGPMPGQVHHFLTVADEADRRYGLARYSAETRRLYGVADRRLAQVPYFAGDLS
                     IADFAILGWAWRLERHRVDLAEFPHVQRWYATMMARPGVQRGFAVALS"
BASE COUNT          311 a          700 c          692 g          303 t
ORIGIN      
        1 gcgatggcgc ggctgagatt gatgcgccgg cgctcctggc tccagcgcag ttcccagttt
       61 cggtcatcgt aggtcacgac ggtgccggtg cgcagccgcc gcttcagcgc ctcgccatgc
      121 tcgccggtga cgtgcgccgc cgcctgctgc agtgcaacct gtacttcggc cagcgccggc
      181 accggcactt ccggcggcac cagttcgtcc aaaatcttgt cgcgccgcaa gtagccgtgg
      241 gccagcacat agtctccaat ggtctggctc tgccgcagcc cgccgcaatg gccgatcatc
      301 agccagcaat gcgggcgcag aacggccaga tgatcggtga tgttcttcgc attggcggga
      361 ccgacgccga tgttgacgat gctgatcccc tcctcgtctc caccacggca gagatgatag
      421 gccggcatct ggaaccggtc ccagggcgct tcctgcgccc gccgctccgc ttccgcccgc
      481 gcctcccctc gccgcacgac gccgccgccc ggcagcacca ggcgttcgta gggtgaatcg
      541 ggatgttgca gctggtcgat gccccagcgt acgaactgat cgacgtaacg ctggtaattc
      601 gtcaacagaa tccagggctg cacggcgcgc cagtcggtgc cggtgtagtg cagcagccgg
      661 cgcagcgagt agtccacccg caccgcgtcg aacagcgcga gcggcagcgg cgccccctcc
      721 ggcgtcgccc agagcccgtc cgcgatctcg tcgccgacgg cggagaggat cggtgtcggg
      781 aaatgccgcg ccagctcgcc gggcgtcacc tgcccctgga acagttcgtc gccgttctcc
      841 agcacatagg gataggggat ctcctgcgcg ctcggcgcga ccgcgaggct ggcgccgaac
      901 tcctccacca gcggatggag ttgctgcagc agatagcggc ggaacgccgc cggctgcgtg
      961 atcgtggtcg cgtaggtgcc cggaacctgc agcttggccc aggcgcggcg cgtgaacggc
     1021 gccggcccgg ccggccgcca ggtcagccgc agttcagggt agcggaaccg cgcccgcgtg
     1081 ccggcatccg gcggcgtgcc gtcggcaacg aagcgagcca gcgccgcacg cagcgcggcc
     1141 accgcctcgc catgcagcgc cgcgagccgg tcaacggcct gttcgggcga aagccccgga
     1201 tggtcggcca cggcgcacat ccctccgcgg ttgcggccgg aggcatggca gccgatgctg
     1261 cgccgggcaa gaggacggag caacgggatg gcgatcgagc tttgggccta cgacacgccg
     1321 aacggtcgca agatcagcat cgcgctggag gagatgggcc tgccctacac ggtgcgggtg
     1381 gtggacatca ccaagggcga gcagcaccgt ccggagttcc tggcgatcag ccccaataac
     1441 cgcattcccg ccatcatcga cccggaggga ccggacggcg ccccgatcag cgtgttcgag
     1501 tcgggcgcgg tcctgctcta tctggcgcag aagacccgga aattctggcc ggctgggctg
     1561 cgcgcgcagg tgccggtgct ggaatggctc atatggcagg tgggcggctt cggcccgatg
     1621 cccggtcagg tgcatcattt cctcaccgtc gccgacgagg ccgaccgccg ctatggcctc
     1681 gcgcgctaca gcgcggagac acggcgcctc tacggcgtag cggaccgccg cctcgcccag
     1741 gtgccgtatt tcgccggcga cctgtcgatc gccgatttcg ccatcctcgg ctgggcgtgg
     1801 cgcctcgagc ggcaccgggt cgatctggcc gagttcccac acgtgcaacg ctggtacgcg
     1861 acgatgatgg cccgtccggg agtgcaacgc ggcttcgcgg tcgccctgtc ctgagcgaga
     1921 cggcgtttga cgtggccggg gcgagaccct agaagcagga cagcaaacgt cgtgtggcaa
     1981 gtctctcccg gcggaggggt ggccga
//
LOCUS       DASSZH010000094         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1366187, whole genome shotgun sequence.
ACCESSION   DASSZH010000094 DASSZH010000000
VERSION     DASSZH010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366187"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(354..>2214)
                     /locus_tag="VFN42_01830"
     CDS             complement(354..>2214)
                     /locus_tag="VFN42_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET8995386.1"
                     /translation="ASEWRAAGRFTGQVARYSVSGSRGVAGAVARGAGMGGQASGWIA
                     DLVRRPGLEEPPRQAAPRVSRPSPMPPLAAPPMEDSVAAPAPAAAAARPPVRSIAPAR
                     KAHQESLPLPEAGWQFPPLSLLKPPPVRASSGPSEDSLQANARLLETVLSDYGVQGSI
                     VEIRPGPVVTLYELEPAPGIRSARVIGLADDVARSLSVTAVRIATVPGRNVIGIEVPN
                     AKRETVFLSEILASEELQKNSGRLALALGKDIGGTTVVADLARMPHLMIAGTTGSGKS
                     VGVNAMILSLLYRLSPDQCRLIMIDPKMLELSTYEGIPHLMAPVVTEPAKAVTALKWT
                     VREMERRYRAMSQLGVRNVGGYNERVEEARRKGEVVTRRVQTGFDPDTGRPTFEDQPL
                     SLEPLPLIVVVVDEMADLMMVAGKEIEAAVQRLAQMARAAGIHVIMATQRPSVDVITG
                     TIKANFPTRISFQVISKFDSRTILGEQGAEQLLGQGDMLFMQGGGRIQRVHGPFVSDE
                     EVEKVVAFLREQGEPAYVEEVTDAESDDAGGGSLLSGIAGASDGEKGLFDQAVAVVAR
                     EGKASTSFIQRHLNIGYNRAAKLIEQMEKEGIVGPANHVGRREVLVRRTHDED"
BASE COUNT          300 a          833 c          709 g          372 t
ORIGIN      
        1 ttacctgcga gcgacaagat tgcacgtttg tccgctgatg aattttggcg cgccgcgcct
       61 ggacgtccgc cgagttgtcc tggctctgtg aaggaacccg ccaccgccgc ccaccgctgc
      121 gcagcctttg cgttctttgc gtttcatctt gcttgcttta cgccgcacag cgccgggtga
      181 cgggcggcga ggtccgtctc cggcgccgcg tcaacaacac ggtgccggaa caacgttcgc
      241 ggggtgccac ccattccttg ccgcactggc gaagcactgg cattgcattg tgttaccggc
      301 ctttggcgta tccacgattt tccttatcag gaactctccc ggctctcgcc ccctcaatcc
      361 tcgtcgtgcg tgcgccgcac cagcacctcg cgccggccga catggttggc ggggccgacg
      421 atgccttcct tctccatctg ctcgatcagc ttggccgccc ggttgtagcc gatgttcagg
      481 tgccgctgga tgaacgacgt gctggccttg ccctcgcgcg ccaccacggc caccgcctgg
      541 tcgaacagcc ccttctcccc atcgctcgcg cccgcgatgc cgctcagcag cgagccgccg
      601 ccggcatcgt cgctctcggc gtcggtgact tcctcgacat aggccggttc gccctgctcg
      661 cgcaggaacg ccacgacctt ctccacttcc tcgtccgaca cgaagggccc gtgcacgcgc
      721 tggatgcgtc cgccgccctg catgaacagc atgtcgccct ggcccagcag ctgttcggcg
      781 ccctgctcgc ccaggatggt gcggctgtcg aacttcgaga tcacctggaa ggaaatccgt
      841 gtcgggaagt tcgccttgat ggtgccggtg atcacgtcca ccgacgggcg ctgagtcgcc
      901 atgatcacat ggatgccggc ggcgcgcgcc atctgcgcga gccgttgcac cgccgcctcg
      961 atctccttgc cggccaccat catcaggtcg gccatctcgt ccaccaccac cacgatcagc
     1021 ggcagcggtt ccaggctcaa cggctggtcc tcgaaggtcg ggcggccggt gtccgggtcg
     1081 aagccggtct gcacgcggcg ggtcaccacc tcgcccttgc gccgcgcctc ctccacccgt
     1141 tcgttgtagc cgcccacgtt gcggacgccg agctgcgaca tggcgcggta gcggcgctcc
     1201 atctcgcgca ccgtccactt cagggcggtc accgccttgg cgggctcggt caccaccggc
     1261 gccatcagat gcgggatgcc ctcataggtg gacagttcca gcatcttcgg gtcgatcatg
     1321 atcagccggc actggtcggg cgacagccgg tacagcagcg acaggatcat cgcgttcacg
     1381 ccgaccgatt tgcccgatcc ggtggtgccc gcgatcatca gatgcggcat gcgcgccaga
     1441 tcggccacca cggtggtgcc gccgatatcc ttgcccagcg ccagcgccag cctgccggaa
     1501 ttcttctgca gctcctcgct ggccaggatt tcgctgagga acacggtttc ccgcttggca
     1561 ttcggcacct cgatgccgat cacgttgcgg ccgggcacgg tggcgatgcg caccgcggtc
     1621 accgacagcg accgcgccac gtcgtcggcc aagccgatca cccgcgcgct gcggatgcca
     1681 ggcgcgggtt ccagctcgta cagcgtcacc accggcccgg gacggatctc cacgatgctg
     1741 ccctgcacgc cgtaatcgct cagcaccgtc tccagcaggc gggcattggc ctgcagtgaa
     1801 tcctcgctcg ggccggagct ggcgcggacc ggcggcggct tcagcaggga cagcggcgga
     1861 aactgccagc ctgcctccgg cagcggcagg ctctcctggt gcgccttgcg ggccggcgcg
     1921 atgctgcgca ccgggggacg cgcggcggcg gcggcgggcg ccggcgcggc cacggagtcc
     1981 tccatcggcg gcgcggccag cggcggcatc ggtgacgggc ggctgacgcg gggcgcggcc
     2041 tgcctcggcg gctcctccag gcccggccgg cgcaccaggt cggcgatcca gccggacgcc
     2101 tggccgccca tgccggcgcc gcgcgccacc gccccggcca cgccgcggct gccggacacc
     2161 gagtagcgcg ccacctgtcc ggtgaagcgc cccgcggccc gccactccga cgct
//
LOCUS       DASSZH010000095         4296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_19750, whole genome shotgun sequence.
ACCESSION   DASSZH010000095 DASSZH010000000
VERSION     DASSZH010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19750"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..906)
                     /locus_tag="VFN42_01835"
     CDS             complement(292..906)
                     /locus_tag="VFN42_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HET8995387.1"
                     /translation="MTRSIDFWFSIGSTYSYLTVMRLNDVQASGGVTFTWCPFNVRDI
                     MVEQNNRPFVGKPVKSAYMWRDIERRAAMYGLPVRVPAPYPLPELEFANRVAILAAEE
                     GWCAEYAIATYQRWFQLGQEPGTEPNLSDSLREVGQDPQRVIAEARAGRIGNALRAAT
                     DRARAAGVFGSPTFVVGTELFWGDDRLDDALAWSRNGSLRNTTN"
     gene            complement(925..1392)
                     /locus_tag="VFN42_01840"
     CDS             complement(925..1392)
                     /locus_tag="VFN42_01840"
                     /inference="COORDINATES: protein motif:HMM:NF023010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HET8995388.1"
                     /translation="MPDPVPPPLQFVFAAFVDIAAPQEIGETTAGRRRVIPITGGHVE
                     GPRLTGTVLPGGADWQLIRSDGTIELVARYTLRASDGTLISVVNRGLRRASPEVLVRL
                     VAGEIVDPALYYFRTAPSFSVAPGPHGWLADSIFVATGERQAKQVVIRFFELG"
     gene            complement(1410..2297)
                     /locus_tag="VFN42_01845"
     CDS             complement(1410..2297)
                     /locus_tag="VFN42_01845"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET8995389.1"
                     /translation="MSSATASSPVEQARFIDFAAGLGAGASFSIADVLGKVVFASGMD
                     VLSLVTLRGVLSATFFWWWLRVKPPRARLTPKTKAAAIGIGLLYAGNVFGLLLAIQLL
                     PLSIAILAYFIYPLLTGISAAAIRLERLGWQSFACAVVAFCGLALMLGTQPGYLAPLG
                     LLGAFGGSICRTVSLLLIRAKLGGTDPRLTTWYTLFPSAVLFVLVTLATGSFHPPLEA
                     SGWFAFCGMSVGTTLSTLLIFISTARVGAFRTAVLLNTEPVMSSAFSFALLGETATGL
                     QLVGGAIMLLALCAFQLRR"
     gene            2574..3092
                     /locus_tag="VFN42_01850"
     CDS             2574..3092
                     /locus_tag="VFN42_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="HET8995390.1"
                     /translation="MPQQPWQAAVYAALKQARITQLGYVPDAGHAHLIEAAHADPDMH
                     PVVLTTEEEGIALAAGAWLGGQRAALLMQSSGVGNCINMLSLPLNCRMPFLTLVTMRG
                     EWGEFNPWQVPMGSATPAVLGAAGVHVRRADQPDEAAEMVAAAASLAFDSAVSVAVLF
                     SQRLIGSKVFVK"
     gene            3089..3676
                     /locus_tag="VFN42_01855"
     CDS             3089..3676
                     /locus_tag="VFN42_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET8995391.1"
                     /translation="MSGANDRTLDRREVVATLLREPGDLLVVTGLGAATYDASAAGDR
                     PLNFYLWGAMGGTAMVGLGLALAQPARRVLVVTGDGDMLMGIGSLATIAAAGAPNLAI
                     AVLDNARFGETGSQRSHTAHTTDLAAVAAACGWRNTATARSTAEVAELAPRLRRENLF
                     AVLRISPEEKPRHLPPRDGAHLADRFRRALGVAVD"
     gene            3692..>4296
                     /locus_tag="VFN42_01860"
     CDS             3692..>4296
                     /locus_tag="VFN42_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HET8995392.1"
                     /translation="MSRGRVAGWLSGGALAAIALLAGIAWLFPPNLSRLGMQGRIVRD
                     RGGTPIAFYQDASGEWKFATSPDAVDPLLIRMLIAIEDHRFYRDPGFDPLAIARAGAQ
                     LLVAGHVVSGASTLTMQIARLLHPAPRTLPVKLREAMQALSLTEHYAKQRIMGMWLSL
                     APFGGNIVGVQAASELWFGKPPRSLSPAQAALLIALCRRPEA"
BASE COUNT          694 a         1488 c         1486 g          628 t
ORIGIN      
        1 gagaggaaca ccagcgtggg ggatctgcca tccagccgtt tccaggcgag ttccacgcca
       61 tcgccacggt ccaggcgtcc ggaagtttcc atcttgtgtt gctcctagta ctgcgttcgc
      121 gaattggcgg cccgaacgaa ccacggcgtg gcgggcgcag gcccgccgcc catgaattgc
      181 tggaggagac tcagcatcgc gaactggctc cctccttcgg cgtttctgcc agccagtcgt
      241 gggtggcgag ccttcgcccg ccacgacgcc gcaccggcac cctgcgcccg ttcagttcgt
      301 cgtgttgcgc aagctgccgt tccgggacca ggccagcgcg tcgtccagcc ggtcgtcacc
      361 ccagaaaagc tccgtgccaa ccacgaaagt tggcgaaccg aagacgcccg ccgcgcgggc
      421 gcgatccgtg gcggcgcgca acgcattgcc gatccggccg gcgcgagctt ccgcgatcac
      481 cctttgcggg tcctgcccca cctcgcgcag gctgtccgag agattgggct ccgtgccggg
      541 ctcctggccc aactggaacc agcgctggta ggtggcgatc gcatactccg cacaccagcc
      601 ttcctccgcg gcgaggatgg caacccggtt ggcgaattcc agctcgggca gtggataggg
      661 cgcggggacg cgcaccggca ggccatacat cgccgcgcgc cgctcgatgt cccgccacat
      721 ataggcggat ttgacaggct tgccgacgaa cgggcggttg ttctgctcca ccatgatgtc
      781 gcgcacgttg aacggacacc aggtgaacgt gacgccgccg ctggcctgca catcgttcag
      841 ccgcatgacg gtgaggtacg agtacgtgct gccgatcgaa aaccagaaat caatggaccg
      901 ggtcatgctt cgcctcctgc aaggtcagcc gagctcgaag aatcgtatca ccacctgctt
      961 tgcctgccgt tcgccggttg ccacgaagat gctgtctgcc agccagccat gcgggccggg
     1021 cgccacgctg aagctgggcg cggtacggaa gtaatacagc gccggatcga cgatttcacc
     1081 ggcgaccagc cgaaccagca cctccggcga tgcgcgccgc aggccgcgat tcaccacgga
     1141 gatcagcgtg ccgtccgacg cgcgcagcgt atagcgcgcc accagctcga ttgtgccgtc
     1201 gctgcggatc agttgccagt cggcgccgcc gggcagcacc gtgccggtca gccgcggacc
     1261 ttcgacatgg ccgccggtga tcgggatgac gcggcgccgg cccgcggtgg tctcaccgat
     1321 ctcctgcggc gcggcgatat ccacgaaggc ggcgaacacg aactgcagtg gcggcggcac
     1381 gggatcaggc atttgcggtc tctccctggt cagcgtcgca actggaaggc gcacagcgcc
     1441 agcagcatga tcgcgccgcc aacgagttgc aggccggtcg cggtttcgcc gagcagcgcg
     1501 aagctgaacg cgctggacat caccggttcc gtgttgagca gcaccgcggt gcggaaagcg
     1561 ccgacccgcg cggtggaaat gaagatcagc agcgtggaaa gcgtggtgcc aacgctcatg
     1621 ccgcagaagg cgaaccaccc gctggcttcc agcggcgggt ggaaactgcc ggtcgccagc
     1681 gtgaccagca cgaacagcac cgcggagggg aacagcgtgt accaggtggt gagccgcgga
     1741 tcggtgccgc ccagcttggc gcggatcagc agcagcgaca cggtgcggca gatcgagccg
     1801 ccaaacgcgc cgagcaagcc cagcggcgcg agataaccgg gctgcgtgcc cagcatcagc
     1861 gccagtccgc agaacgcgac caccgcgcag gcgaagctct gccagcccag ccgctccagc
     1921 cggatcgccg ccgcggagat gccggtgagc agcggataga tgaaataggc caggatcgcg
     1981 atcgacagcg gcaagagctg gatcgccagc agcaggccga acacattgcc ggcatacagc
     2041 aagccgatgc cgatcgcggc ggccttggtc ttcggcgtga gccgggcgcg gggaggcttc
     2101 acgcgcagcc accaccagaa gaacgtggcg gacaggacgc cgcgcaatgt caccagcgac
     2161 agcacgtcca tgccggaagc gaacaccacc ttgcccagca cgtcggcgat gctgaaggac
     2221 gcgccggcac ccaggccggc ggcgaaatcg atgaaacggg cctgctcgac cggtgaactg
     2281 gcggtggcgc tgctcattgg acgctgtcag cccttcgcca ggatgcgcgg gcagggcgga
     2341 tcgcgtcgcg gcgatgcccg cctgcgaccg cggcaccagg ccggcctgcg gccttcggcg
     2401 ccgccaggat ggcgcgttct tcgtcggtcg gctgcatgcg ggtgtcctgg ttccggtgtc
     2461 ccgggactgt gcggggaacc gcaggcggac gcaaaccggc tgggcgggcg gaccccgcac
     2521 ttgcggcgcg cgcgatcgtg acatagccct gggcgcgacc cacaggagac cccatgccgc
     2581 aacaaccctg gcaggcggcg gtctatgccg cgctgaagca ggcccgcatc acgcagctcg
     2641 gctacgtgcc ggatgccggc cacgcccacc tgatcgaggc ggcacatgcc gaccccgaca
     2701 tgcatcccgt cgtcctcacc accgaggaag aaggcatcgc cctcgccgcc ggcgcctggc
     2761 tcggcggaca acgcgcggca ctgctgatgc agtcctcggg cgtgggcaac tgcatcaaca
     2821 tgctctcgct gccgctgaac tgccgcatgc cgttcctcac cctggtgacg atgcgcggcg
     2881 agtggggcga gttcaatccg tggcaggtgc cgatgggcag tgcgacgccc gccgtgctcg
     2941 gcgccgccgg cgtgcatgtc cgccgcgccg accagccgga cgaggccgcg gagatggtcg
     3001 ccgccgctgc ctccctggct ttcgacagcg cggtctccgt ggcggtgctg ttctcgcaac
     3061 ggctgatcgg cagcaaggtg ttcgtgaaat gagcggtgcc aacgacagaa ccctggaccg
     3121 ccgcgaggtg gtggcgacgc tgctgcgcga gccgggcgac ctgctggtgg tgaccggcct
     3181 gggtgccgcc acgtacgatg ccagcgccgc cggcgaccgg ccgctgaact tctatctgtg
     3241 gggcgcgatg ggcggaaccg ccatggtcgg cctcggcttg gcgctggcgc agcctgcgcg
     3301 ccgcgtgctg gtggtcaccg gcgacggcga catgctgatg ggcattggtt cgctcgccac
     3361 catcgccgcg gcgggggcac caaacctggc gattgccgtg ctggacaatg cgcgcttcgg
     3421 cgagaccgga tcgcagcgca gccataccgc gcataccacc gatcttgcgg cggtggcggc
     3481 ggcctgcggc tggcgcaaca ccgcgaccgc ccgcagcacg gcggaggtgg cggaactggc
     3541 gccgcggctg cggcgggaaa acctgtttgc ggtgctgcgg atttcgccgg aggaaaagcc
     3601 gcgccatctg ccgccgcgcg acggcgcaca cctggcggat cgcttccgcc gtgcgctggg
     3661 cgtcgcggtg gactgacagg cggcgcgcgc aatgagccgt gggcgcgtcg ccggctggct
     3721 gtccggcggc gcccttgccg ccatcgcgct gctcgccggc atcgcctggc tgttcccacc
     3781 gaacctctcc cgcctgggca tgcaagggcg catcgtgcgc gaccgcggcg gcacgccgat
     3841 cgcgttctat caggacgcaa gcggcgaatg gaaattcgcc acctcgccgg atgcggttga
     3901 tccgctgctg atccgcatgc tgatcgcgat cgaggaccac cgcttctacc gcgatcccgg
     3961 cttcgatccg ctggcgatcg cccgagccgg cgcgcagttg ctggttgccg ggcatgtcgt
     4021 gtccggcgcc tccacgctta ccatgcagat cgcccgcctg ctgcatcccg cgccgcgcac
     4081 gctgcccgtg aaactgcgcg aggcgatgca ggcgctgtcg ctgaccgagc actatgccaa
     4141 gcagcgcatc atgggcatgt ggctgtctct ggcgccgttc ggcggcaaca tcgtcggcgt
     4201 gcaggcggca tcggagctgt ggttcggcaa gccaccgcgc agcctcagcc cggcccaggc
     4261 ggcgttgttg atcgcgctgt gccggcggcc ggaggc
//
LOCUS       DASSZH010000096         3634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_156038, whole genome shotgun sequence.
ACCESSION   DASSZH010000096 DASSZH010000000
VERSION     DASSZH010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156038"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VFN42_01865"
     CDS             complement(<1..608)
                     /locus_tag="VFN42_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HET8995393.1"
                     /translation="MRRLLLILLLLLLAAPAAGQVRIKDIVDIEGIRQNQLIGYGLVV
                     GLNGTGDKLDNSVFTRESLIGMLERLGVNTRDQAAKLQTKDVAAVMVTAELPAFARSG
                     SRIDVSVSALGDASNLTGGTLLVTPLLAADGEVYAVAQGALATGAIAARGAASSVTRG
                     VPTAGRIANGATVEREVAYRLDKASSLRLGLRNPDLTTARRIA"
     gene            767..1171
                     /locus_tag="VFN42_01870"
     CDS             767..1171
                     /locus_tag="VFN42_01870"
                     /inference="COORDINATES: protein motif:HMM:NF022227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliX"
                     /protein_id="HET8995394.1"
                     /translation="MSLIEGLGAPAPGRLPNRTPRSGTASFTLDTAAESAGTGAAQAA
                     APPLAVSALALQEVAIDAPRDRAARRHGQALLAALGRLQRHLLGGADAAGSLDELAAL
                     AAIVPNVDEPGLAATLHATVLRARVELARRGR"
     gene            1339..1752
                     /gene="dksA"
                     /locus_tag="VFN42_01875"
     CDS             1339..1752
                     /gene="dksA"
                     /locus_tag="VFN42_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HET8995395.1"
                     /translation="MVTLPPDYRPTEDEEFMNPIQVEYFRQKLLRWRADLLKEADGTL
                     ASLSEGGIHEADITDRASVETDRALELRTRDRARKLIAKIDQALLRIEAGTYGYCEET
                     DEPIGLRRLEARPIATLSIEAQERHERMERVHRDD"
     gene            1851..2195
                     /locus_tag="VFN42_01880"
     CDS             1851..2195
                     /locus_tag="VFN42_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995396.1"
                     /translation="MQRTLTVLLCTGLALAPWAASQAQNPQTAPPGNPHPATASPSAP
                     TPPPEKLSPAAKADTNRTTLSDKLAQQNGTLPPPKTGDRSAVIKPHQEAGNMPVIPPP
                     GTKGGNQRVQPK"
     gene            complement(2401..>3634)
                     /locus_tag="VFN42_01885"
     CDS             complement(2401..>3634)
                     /locus_tag="VFN42_01885"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET8995397.1"
                     /translation="DTAMGRTLRRYWMPALLSSEIPEPDGPPVRVRLLGEDLVAFRDS
                     QGRIGLLDELCPHRRASLYFGRNEECGLRCVYHGWKFDVAGQCTDMMNEPEENDFSHK
                     VRTPAYPTCEMGGVIWAYLGPAEKMPPLPKFAWTQAGEAHRHVTKVIQECNWLQGLEG
                     GIDTSHSPILHRLISETSTRGGFKPSSPFVRGKAPQLVVDLTDYGFQYAGIRPLGDAE
                     RHIRTYQFILPFHQIRPAKSESGLPLDAGHCWVPMDDGTTMVFNWSYCPDGPLGEEDR
                     LERSLGNGPLHVDQKTFRPVANRANDYLLDRTVQRTESYSGIDGINVQDRAIQESMGR
                     IVDRSKEHLGPADKAIIQARKLLRQAVKAVEAGGAPDGTGPSYYPLRAHEAILPREAD
                     WRREMTPELREGAILQTV"
BASE COUNT          600 a         1282 c         1222 g          530 t
ORIGIN      
        1 gcgatccggc gcgccgtggt caggtccgga ttgcgcagcc ccagccgcag gctgctggcc
       61 ttgtccaggc ggtaggcgac ctcccgctcc accgtcgcgc cattggcgat ccgtccggcg
      121 gtgggcacgc cacgggtgac ggagctggcg gccccgcgtg cggcgatcgc gccggtggcc
      181 agcgcgccct gcgccaccgc atagacctcg ccatccgccg ccagcagcgg ggtgaccagc
      241 aaggtcccgc cggtcaggtt ggacgcatcg cccagcgccg agaccgacac gtcgatccgg
      301 ctgccgctgc gggcaaaggc cggcagctcg gcggtcacca tcaccgcggc gacgtccttg
      361 gtctgcagct tggccgcctg gtcgcgggtg ttcaccccca ggcgttccag catgccgatc
      421 agcgattccc gggtgaacac ggaattgtcc agcttgtcgc cggtgccgtt cagcccgacc
      481 accaggccgt agccgatcaa ctggttctgc cggatgccct cgatatcgac gatgtccttg
      541 atccgcacct gcccggcggc gggagcggcg agcagcagca acagcaggat gagcagcaga
      601 cggcgcatgg cggttagccg gctcgaaagg attttgcggc actctgccgc cggaaccgca
      661 agctgcgtgc caggcgggca tccggcccac accgggccgg tttgcgccgg gcaaagccgg
      721 cagaacatgc cgggcggcgc gccgcggcag gaaaaaggag ccggttgtgt cgctcattga
      781 agggctgggc gcccccgcgc cgggtcggct gccgaaccgc acgccacgga gcgggacggc
      841 atcgttcacg ctggacaccg ccgcggaatc agccggaacc ggcgcggcgc aggcagcagc
      901 accgccgctg gcggtcagcg cgctcgcctt gcaggaagtg gcgatcgacg caccgcggga
      961 ccgcgccgcg cgccggcacg gccaggcgct gctcgccgcg ctgggccgcc tgcagcgcca
     1021 cctgctcggc ggggcggatg cggcgggaag cctggacgag ctggctgcgc tggcggcgat
     1081 tgttccgaat gtggacgaac ctggcctcgc ggcgaccttg cacgcaaccg tactgcgcgc
     1141 gcgcgtggaa ctggcccgcc gcggccgctg aatcgcagcg tttgattgca agtgcttgaa
     1201 acctgcgcgt tggccttggc agaggcgcga atgcgtggct ataagccggc gcgttcacga
     1261 cccggaatgg gccgggggga aatctgccgc tggcggcggc agcaagagaa aacggacgag
     1321 caggacacga cacgcatgat ggtgacgctg ccccccgact accgcccgac cgaggatgag
     1381 gagttcatga accccatcca ggtcgaatat ttccggcaga agctgctgcg ctggcgtgcc
     1441 gatttgctga aggaggcgga cggtaccctg gccagcctgt ccgaaggcgg cattcacgaa
     1501 gccgacatca ccgaccgtgc cagtgttgaa acagatcgtg cgctggaact gcggacacgt
     1561 gatcgcgccc gcaagctgat cgccaagatc gaccaggcgc tgctgcgcat cgaggccggc
     1621 acctacggct actgcgagga aaccgacgag ccgatcggcc tgcgccggct ggaggcgcgt
     1681 ccgatcgcca cgctgtccat cgaggcgcag gaacgccacg aacgcatgga gcgcgtccac
     1741 cgcgacgact gaggcctgat ccctccgccg gcaaccttgt gcggcttcgg ccgttcccac
     1801 ctgttcgagc ggctgttcga cagccgcggc cgcaacaacg gagtgggatc atgcaacgaa
     1861 ccctgaccgt gctgctgtgc accggcctcg ccctggctcc ctgggcggcg agccaggcac
     1921 aaaatccgca aaccgccccg ccggggaatc cgcatccggc caccgcctcg cccagcgcgc
     1981 cgactccgcc gccggagaag ctctcgccgg ccgcaaaggc cgacaccaac cggacgacgc
     2041 tgagcgacaa gctggcgcag cagaacggca ccttgcctcc gccaaagacc ggcgaccgca
     2101 gcgcggtgat caagccgcac caggaggcgg gcaacatgcc ggtgatcccg ccgccgggca
     2161 ccaaaggcgg caatcagcgg gtgcagccga agtagcccgg caagcgcccg caccggcggg
     2221 cagaacaatg ggcccgcctg gattgggatc cgcactccgt cgtgccggcg aatgccggca
     2281 cccatggctt ctcctgatct gcatgcgaca aagtcatggg tggcggccct tcgcccgcca
     2341 cgacgccgaa cgctgcatcc cgaccgaaag cccgcgagtc acccaacccg cgggttgagc
     2401 tcacaccgtc tgcaggatcg cgccttcgcg cagttccggc gtcatctcgc gccgccaatc
     2461 cgcctcgcgc ggcaggatcg cctcatgggc gcgcagcgga taatagctgg gtccggtgcc
     2521 gtccggcgcg ccgccggcct ccaccgcctt caccgcctgg cgcagcagct tgcgcgcctg
     2581 gatgatggcc ttgtccgccg gtcccaggtg ctccttcgaa cgatccacaa tccgtcccat
     2641 gctttcctgg atcgcgcggt cctgcacgtt gatcccgtcg atgccgctgt agctctcggt
     2701 gcgctgcacc gtgcggtcca gcaggtagtc gttggcccgg ttggcaaccg ggcggaacgt
     2761 cttctggtcc acatgcagcg ggccattgcc caggctgcgc tccagccggt cttcctcgcc
     2821 cagcggaccg tccggacaat acgaccagtt gaacaccatg gtggtgccgt catccatcgg
     2881 cacccagcaa tgcccggcat ccagcggcag tccgctttcc gacttcgccg gccggatctg
     2941 atggaacggc aggatgaact ggtaggtgcg gatgtggcgt tccgcatccc ccagcggacg
     3001 gatgccggcg tactggaacc cgtaatcggt caggtccacc accagttgcg gcgccttgcc
     3061 acgcacaaac gggctggacg gcttgaaccc accccgggtg gaggtttcgc tgatcagccg
     3121 gtggagaatc ggcgagtgcg aggtgtcgat gccgccttcc aggccctgca gccagttgca
     3181 ctcttggatc accttggtga cgtgccggtg ggcttcgccg gcctgcgtcc aggcgaattt
     3241 cggcagcggc ggcatcttct ccgccgggcc gagataggcc cagatcaccc cacccatctc
     3301 gcaggtcgga taggccggcg tacgcacctt gtgggagaaa tcgttctcct ccggctcgtt
     3361 catcatatcg gtgcactggc cggccacgtc gaacttccag ccatgataga cgcagcgcag
     3421 cccgcattcc tcgttgcggc cgaaatacag cgaggcgcgg cggtgcgggc acagctcgtc
     3481 cagcaatccg atccggccct ggctgtcgcg gaacgcgacc aggtcctcgc cgagcaggcg
     3541 aacgcggacg ggtgggccgt cgggctcggg gatctccgag gacagcagcg ccggcatcca
     3601 gtagcggcgc agggtgcggc ccatggccgt gtcg
//
LOCUS       DASSZH010000097         2881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_207211, whole genome shotgun sequence.
ACCESSION   DASSZH010000097 DASSZH010000000
VERSION     DASSZH010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207211"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VFN42_01890"
     CDS             <1..334
                     /locus_tag="VFN42_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019088293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8995398.1"
                     /translation="WNLALEGITGFTVMPLKFATYLGLLVAALATAFGLQVVVKTLLF
                     GNPVAGYPSLMTVVLMLGGVQLMTLGVIGEYLGRIFNETKQRPLYLVEHFIPASTRAS
                     VAEREPVG"
     gene            331..1419
                     /locus_tag="VFN42_01895"
     CDS             331..1419
                     /locus_tag="VFN42_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HET8995399.1"
                     /translation="MIRRRILLLLACCVFGWAARADAPADRVARLGRGINLTNWFRFP
                     PSLAPDALRTYLSDAVVLQLRRAGFTAVRLSVQPALFGTLPGIRLLTAAVRRLQQGGL
                     AVVVAAHPDGWHLETSANDRARLLAFWSMLAPALGSLDVRLTFPELLNEPVFPGNPEA
                     WRRLQHTLLETVRSALPEHTIVLSGNDWGSIAGLLALRPEADPNVIYSIHYYDPPELT
                     SLAAYRPGLDRASLADLPFPAANRAACFQVAARSSDPATAALIRFYCGLHWDAARIAS
                     RFAAAASWARRNHASLLLGEFGASAALNPLARLNWLSAVRRQAEAAKISWMLWGYDDV
                     MGFALSRPPPARPVLDTAVLRALGLTGR"
     gene            1518..1802
                     /locus_tag="VFN42_01900"
     CDS             1518..1802
                     /locus_tag="VFN42_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995400.1"
                     /translation="MSQIVPLSSHRDDTLWAVVASLGRTTRVAEVFSNRLAAVADKAW
                     REQQVKAYANFLRMSRQPPPTYSVTPIRRSDLPRAWRPLPALGFLHGQFI"
     gene            complement(1877..>2881)
                     /locus_tag="VFN42_01905"
     CDS             complement(1877..>2881)
                     /locus_tag="VFN42_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003617821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET8995401.1"
                     /translation="VCADGRLLDLVRAEGFRVITVPMQRRVAPLAHLHALRHLVAVMR
                     AERPDLVHAHMPISGFLARIAARIAGVPRVAYTCHGFLFNYQTAWLHRAAGLLMECVA
                     APLTDVYLTVAESEAADARRLHFSRTAVAVGNGRDPARFRPDPEARARLRAAFGTPAE
                     RPVVIAVSRLVRLKGYPELAAAMQAVPEAELWVVGERLDSDRGDDMATLLANAGLGDR
                     LRLLGYRDDVASLLAAADIFVLPSHFEGLPMSVIEAMLVGLPVIASELPGTREQVVPE
                     LTGLLVPPRQSAPLAAALERLVGDPALRARMGDAGRARGLERFDEAKVIARTLDLLGL
                     "
BASE COUNT          428 a          989 c          980 g          484 t
ORIGIN      
        1 gtggaacctg gcgctggaag ggatcaccgg gttcaccgtg atgccgttga agtttgccac
       61 ctatctcggt cttctggttg ccgctctggc caccgccttc ggtctgcagg tggtggttaa
      121 gaccctgctg ttcggcaatc cggtcgccgg ctaccccagc ctgatgaccg tcgtcctgat
      181 gctcggcggc gtgcaactca tgacgttggg agtcatcggc gagtacctcg gccgcatttt
      241 taacgaaacc aagcagcggc ccttgtacct ggtggagcac ttcatcccgg cgagcacacg
      301 cgcatccgtg gcggaacgcg agccggtcgg atgatccgcc gccgcatcct gctgctcctg
      361 gcctgctgcg tgttcggctg ggcggcgcgg gcggatgctc cggccgaccg cgtggcgcgg
      421 ctcggtcgcg gcatcaacct gaccaattgg ttccgctttc cgcccagcct cgcaccggac
      481 gcgttgcgaa cctatttgag tgacgccgtg gttctccagt tgcgccgggc ggggttcacg
      541 gcggtgcggc tgtcggtcca gccggcgctg tttggtacgc tgcccggcat ccggctgctc
      601 accgccgcgg tgcgccgact ccagcagggc ggactggcgg ttgtggtggc ggcgcatccc
      661 gatgggtggc acctggaaac cagcgccaat gaccgcgcca gactgctggc cttttggtcc
      721 atgctggcgc ccgcgctggg ttctctcgac gtccgactca cctttccgga gttgctgaac
      781 gagccggtct ttccgggcaa cccggaggca tggcggaggc tgcagcacac gctgctggaa
      841 accgtccggt cagcgttgcc cgagcacacc atcgtgctca gtggcaatga ctggggtagc
      901 atcgccgggc tgttggcgct gcggccggaa gccgatccga acgtgatcta cagcatccac
      961 tattacgatc caccggaact gacgtcgttg gctgcctacc ggccgggcct ggaccgggcg
     1021 tcgctggcgg atttgccttt tccggcggcg aaccgcgccg cctgctttca ggtcgccgcc
     1081 cggagcagcg atccggcgac tgcggcgctg atccggttct attgtggcct gcattgggat
     1141 gccgcgcgca ttgcctcccg tttcgccgct gctgcctcct gggcccgccg caaccacgca
     1201 tcgctgctgc tgggcgagtt cggcgccagc gccgcgttga acccccttgc acggttgaac
     1261 tggctgtcgg cggtgcggcg gcaggccgag gcggcaaaga tcagctggat gctgtggggc
     1321 tacgacgatg tgatgggctt cgcgctcagc cgtccgccgc cggcccgccc cgtgctggac
     1381 accgcggtcc tgcgtgcctt gggactgact gggcgatgac ctcagcccaa ccgatgcggg
     1441 gaagcaggcc ggggcgtggg aatgcctctg tgggaatgcc tcttgtcggg cgggcgggga
     1501 cgcgccagcc tttgcccatg agccagatcg tacccttgag ttcgcatcgt gacgacaccc
     1561 tctgggccgt cgttgcgtcg ctgggccgta ccacgcgggt cgccgaggtt ttcagcaacc
     1621 ggctggccgc tgtggccgac aaggcgtggc gggagcagca ggtgaaggcc tatgccaatt
     1681 tcctgcgcat gtcgcggcaa ccgcccccga cctactcggt aacgccgatc cgccgctccg
     1741 acctgccccg ggcttggcgt ccgctgccgg cgctgggatt cctgcacgga caattcatct
     1801 agccgccgcc aacgaagcac agcggcgtgc tgtgaccact tcaccgggta tcgacggtcc
     1861 agcggaactg cgccggctat agcccaagca ggtcgagcgt ccgggcgatc actttcgcct
     1921 catcgaaccg ctccaggcca cgagcgcgtc cggcgtcccc catgcgcgcg cgaagcgcgg
     1981 ggtcgcctac cagccgctcc agtgcggcgg caagcggcgc gctctggcgc ggtggcacca
     2041 gcaggccggt gagctcggga accacctgct cgcgcgtgcc gggaagttcg ctggcaataa
     2101 ccggcagacc gaccagcatg gcttcaatca ccgacatggg caggccttca aaatggctcg
     2161 gcagcacgaa tatgtccgct gccgccagca gcgatgccac gtcatcgcga tagccgagca
     2221 ggcgcaggcg atcgccgagg ccggcattcg cgagcagggt ggccatatcg tcgccgcgat
     2281 cggagtccag ccgttcgccg accacccata actccgcctc gggcacggcc tgcatggccg
     2341 cggcaagctc gggatagccc tttaggcgga cgaggcggga caccgcgatc accaccggcc
     2401 gctctgccgg cgtcccgaac gccgcgcgga ggcgtgcgcg tgcctcggga tcgggccgga
     2461 agcgcgcggg gtcccggcca ttgccgaccg ccacggcagt acgggagaag tgcaggcgcc
     2521 gggcatcggc ggcctcgctc tcggccaccg tgaggtacac gtcggtcagc ggtgcggcca
     2581 cgcattccat cagcagcccg gcagcgcgat gcagccaggc agtttggtaa ttgaacagga
     2641 acccgtggca ggtataggcc acgcgcggca ccccggcgat gcgtgccgcg atccgcgcga
     2701 ggaagccgct gatcggcatg tgggcatgca ccaagtccgg ccgctctgcc cgcatcaccg
     2761 cgaccaggtg gcgcaaggcg tgcagatgcg ccaggggcgc gacacggcgt tgcatcggca
     2821 cggtgatcac gcggaaccct tccgcccgta ccagatccag cagccgtccg tcggcacaga
     2881 c
//
LOCUS       DASSZH010000098         2434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_496930, whole genome shotgun sequence.
ACCESSION   DASSZH010000098 DASSZH010000000
VERSION     DASSZH010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496930"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1213
                     /gene="queG"
                     /locus_tag="VFN42_01910"
     CDS             134..1213
                     /gene="queG"
                     /locus_tag="VFN42_01910"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HET8995402.1"
                     /translation="MDQHPPLTDPRQAIRQRALALGFDAIGFAAAELGPEVREHLGAF
                     LAAGQHGDMGWLADRAAQRAHPQALWPEARSVIALGLCYAPEDDPLAALDRPDRGTIS
                     VYARNRDYHDVLKGMLKHLAQFIASRFAAGVKVFVDTAPVMEKPLAVRAGLGWQGKHT
                     NLVSRRHGSWLFLGEVYTTLALSPEPSHADRCGTCTRCLSACPTGAFPAPYRLDATRC
                     ISYLTIEHHGPIPHELRPLMGNRIYGCDDCLAVCPWNRFARATPHDKLRAREELSAPR
                     LAALAMLDDAGFRAMFAGSPVKRIGRDRFVRNVLIAIGNSRDAGLAPSAERLRGDADP
                     SVAEAAEWACVRLRAGGAGGSDPGT"
     gene            complement(1262..2059)
                     /locus_tag="VFN42_01915"
     CDS             complement(1262..2059)
                     /locus_tag="VFN42_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995403.1"
                     /translation="MSLPPYHVAAYNTAKSSENKIHDDAIAQRFGFRGGLVGGVHVYA
                     YMTHLPVQHWGRAWLERGTGEARFGKPVYEGDIAEINAQEDAGGLTLTVQSAGVLCAT
                     GHAALPAAAPAISIADFRAVPARTTRPAADEQSLRVGDWLGMTPLTVTPEYQVEDLAD
                     TRETDPIYAAEGIVHPGTILRCCNWALAHNVVLPAWMHMGSTVQNLGIARVGDTLGVR
                     ARVTKNYEHKGHKWVEIDALVLANETTPLARVTHIAIYRPRQLAEAA"
     gene            complement(2218..>2434)
                     /locus_tag="VFN42_01920"
     CDS             complement(2218..>2434)
                     /locus_tag="VFN42_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995404.1"
                     /translation="ERGLISEESDQHQYRFVDIVDVDTNKSLFQIEHETRSMRHPMFN
                     RTFVNRNPDMNNRNFVPGYEVGLWAMT"
BASE COUNT          343 a          814 c          860 g          417 t
ORIGIN      
        1 gtaaggatca gccgtggcgc gggcacgaca caggcgttgg cgggttcgtc gccggccata
       61 acctcgcgcc cgccaccatc ggtccctatg tggaagctgc gccggggcat tttggcccgg
      121 cgtccggatc agcatggacc agcacccgcc tctcaccgac ccccggcaag ccatccgcca
      181 gcgcgccctg gcgctcggct tcgacgccat cggcttcgcg gccgcggaac tcggtcccga
      241 ggtgcgcgaa cacctgggcg cgttcctggc cgcgggacag catggcgaca tgggctggct
      301 ggccgaccgc gcggcgcaac gcgcgcatcc ccaggccttg tggccggagg cgcgcagcgt
      361 gatcgcactg ggactgtgct acgcgccgga ggatgatccg ctcgcggcgc tggaccgtcc
      421 tgatcgcggg acgatctcgg tgtatgcccg caatcgcgat tatcatgacg tgctgaaggg
      481 catgctgaag cacctggcgc aatttatcgc gtcgcgtttc gcggccgggg tgaaagtatt
      541 cgtggacacc gcgccggtga tggaaaagcc gctcgcggta cgggccgggc tgggctggca
      601 gggcaagcac accaacctgg tgtcgcgccg gcacgggtcg tggttgttcc tgggcgaggt
      661 ctacaccacg ctcgctctgt cccccgagcc atcgcatgcc gaccgctgcg gcacctgcac
      721 gcgctgcctg tccgcctgtc cgacaggcgc gttcccggcg ccgtaccggc tggatgcgac
      781 gcgctgcatt tcctacctga cgatcgaaca tcacgggccg atcccgcacg aattgcgtcc
      841 gctgatgggc aaccgcatct atggatgcga cgactgcctg gcggtctgcc cgtggaaccg
      901 cttcgcgcgg gccaccccgc acgacaagct gcgggcgcgg gaggagctca gcgcgccacg
      961 gctggcggcg ctggccatgc tggacgatgc agggttccgc gcgatgttcg cgggctcgcc
     1021 ggtcaagcgc atcgggcgcg atcgcttcgt ccggaacgtg ctgatcgcga tcggcaattc
     1081 gcgggatgcc ggattggcgc cgagcgcgga acggctgcgc ggggatgcgg atcctagcgt
     1141 tgccgaggcg gcggaatggg cgtgcgtgcg gttgcgcgct ggcggcgccg gcggctcaga
     1201 ccccgggacg taaccggttg gtgttgccgg ccggcgaaat ccggccgtcg cacgacgatg
     1261 ctcaggccgc ctcggccaat tgccgcggcc ggtagatcgc gatgtgggtc acgcgggcca
     1321 gcggcgtggt ttcattcgcc agcaccagcg cgtcgatctc cacccatttg tggcccttgt
     1381 gctcgtagtt cttcgtcacg cgcgcacgca cgcccagcgt gtcgccgacg cgcgcgatgc
     1441 cgaggttctg caccgtgctg cccatgtgca tccaggccgg cagcaccacg ttgtgcgcca
     1501 gcgcccagtt gcagcagcgc aggatcgtgc cgggatgcac gatgccctcg gcggcgtaga
     1561 tcgggtcggt ctcgcgggtg tccgccagat cctccacctg atattccggg gtcacggtca
     1621 gcggcgtcat gccgagccag tcgccgacgc ggagggattg ctcgtcggcc gccggacggg
     1681 tggtgcgggc cggcaccgcg cggaaatcgg cgatcgagat ggctggtgcg gcggccggca
     1741 atgcggcgtg gccggtggcg cacagcacgc cggcgctctg caccgtcagg gtcagtccgc
     1801 cggcgtcttc ctgcgcgttg atctcggcta tgtcgccttc gtagaccggc ttgccgaagc
     1861 gtgcctcacc ggtgccgcgt tccagccagg cgcggcccca gtgctgcacc ggcaggtgcg
     1921 tcatgtaggc gtagacgtgc acgccgccga ccaggccgcc gcggaatccg aagcgctgcg
     1981 cgatggcgtc gtcgtggatc ttgttctccg acgatttcgc cgtgttgtag gcggctacgt
     2041 gatagggcgg cagagacatc gagggctcct gcaaagctgc gcgccggggc cggcagcgca
     2101 cgccccgccg acgtatggtc ggcgttcaaa cgggcggcgc tgcgtcccgc agcaagtcgt
     2161 ggatggacgg ccttcgccgg gcatgacggg tcgggccttc gctcgccagc cgttggttca
     2221 ggtcatcgcc cacaggccga cttcgtagcc cggcacgaag ttgcggttgt tcatgtccgg
     2281 attacggttg acgaacgtcc ggttgaacat cggatgacgc atgctgcgcg tctcgtgctc
     2341 gatctggaac agcgacttgt tggtatcgac atcgacgata tcgacaaacc ggtactgatg
     2401 ctggtcgctt tcctcgctga tcagcccgcg ctcg
//
LOCUS       DASSZH010000099         6794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1110734, whole genome shotgun sequence.
ACCESSION   DASSZH010000099 DASSZH010000000
VERSION     DASSZH010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6794
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110734"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VFN42_01925"
     CDS             <1..240
                     /locus_tag="VFN42_01925"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8995405.1"
                     /translation="WILSRRGVLDLARSWALISENPAAAAGLTDRGRIAPGLRADLVV
                     MEPDAPRIVATVASGRVTWLGAEGASRFRDELIRS"
     gene            372..584
                     /locus_tag="VFN42_01930"
     CDS             372..584
                     /locus_tag="VFN42_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995406.1"
                     /translation="MKVRMRLALPLVLTVAGCATTTPPLANSPGPVAQTSAAPPQRVS
                     AASDALGHQLDSMLANYRPASTTLTR"
     gene            745..942
                     /locus_tag="VFN42_01935"
     CDS             745..942
                     /locus_tag="VFN42_01935"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HET8995407.1"
                     /translation="MPIQCRTKTEHRNNRRAVTALEYALIAAAVALAISSSVIVLGSQ
                     LDRTLDHLWDLMRQSYSHRSG"
     gene            complement(1018..1093)
                     /locus_tag="VFN42_01940"
     tRNA            complement(1018..1093)
                     /locus_tag="VFN42_01940"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1058..1060),aa:Asn,seq:gtt)
     gene            complement(1375..1590)
                     /locus_tag="VFN42_01945"
     CDS             complement(1375..1590)
                     /locus_tag="VFN42_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995408.1"
                     /translation="MTDDDEPSATDCHDAAIGILQGLHMLAEEADHLRLSRTYVALCK
                     AIRACQAEHARMMPGARPRPRRYATLH"
     gene            1678..2574
                     /gene="lpxI"
                     /locus_tag="VFN42_01950"
     CDS             1678..2574
                     /gene="lpxI"
                     /locus_tag="VFN42_01950"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019090577.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HET8995409.1"
                     /translation="MLEFPPAATATELAAEKPRTLGILAGGGTLPAQVARAARAAGRD
                     VFIVGLDGFAERAVLAEWPHEIIRMGAAGRILATLRAHDCQDIVLIGPVRRPSLLDMR
                     PDAEGARILARVGRAAFAGDDGLLAAVIRVLGEEGFQVIGAHEVLRAPLAPGGVLTRV
                     APDAQALADIARGVAVVRALGRVDVGQGCVVQQGIVLAVEAAEGTDAMLARVAPLRRD
                     GPGGVLVKLVKPGQDRRADLPTVGPQTVRCAAAAGLRGVAFEAGGTILAESEATIAAA
                     DAAGLFLLGLDPGTIPEGSEGE"
     gene            2571..2957
                     /gene="gloA"
                     /locus_tag="VFN42_01955"
     CDS             2571..2957
                     /gene="gloA"
                     /locus_tag="VFN42_01955"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HET8995410.1"
                     /translation="MSRFLHTMLRVGDLQRSIDFYTKAFGMKELRRRDVPDGKYTLAF
                     VGFGDEDSSTVIELTYNYGVEKYEMGGAFGHLAIGVPDVAAACEVAAKAGGTITRPAG
                     PVKFGTTIIAFVKDPDGYAIELVQRA"
     gene            complement(2998..3555)
                     /locus_tag="VFN42_01960"
     CDS             complement(2998..3555)
                     /locus_tag="VFN42_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HET8995411.1"
                     /translation="MADISGRKIAVLATDGVEQVELTEPVKALKQAGAEVVVIAPHGG
                     QIQGMNHHDKADKLPVDQELDKASPERFDGLVLPGGVANPDMLRTIPQAVALVRHFVQ
                     SGKPIAAICHGPWTLIEAEGVRGRTMTSWPSLRTDLRNAGATWEDKTVIRDGKLVTSR
                     KPDDLPAFCREMVKLFGEEPARKAA"
     gene            3637..4551
                     /locus_tag="VFN42_01965"
     CDS             3637..4551
                     /locus_tag="VFN42_01965"
                     /inference="COORDINATES: protein motif:HMM:NF016903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnan synthesis F family protein"
                     /protein_id="HET8995412.1"
                     /translation="MRTHRVERLLPYASAPLGRFRSHRQIGFAWPEGAVRLGPRIALV
                     CHYDAGGRLRPDLLAYLRALRDAGFSVVLVSNSGTMHPDALQAGRALCAAVLVRRNLG
                     LDFCAWRDAMEWLGLPHAGTAQLLLANDSVYGPLRPLAPLLARIGDAPGLWGMTDSVE
                     RGFHLQSYFLLAQADVIRSAAWQRFWRGVRPLRSKWLMIGLYEVGLTQRLLRTGLGCH
                     ALFSGVAGDGKLFANPTLDGWRILLDAGFPFLKRELLRDNPTLVPDLAAWRTRVTEIA
                     GARAVAWIEADLAGRRTQTGLSNSAAAL"
     gene            4585..>6794
                     /locus_tag="VFN42_01970"
     CDS             4585..>6794
                     /locus_tag="VFN42_01970"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET8995413.1"
                     /translation="MPYRGQSLRRIEDARFLTGGARYVEDIVAAGDAWMQVVRSPHAH
                     AELLRIDTTAAAAMPGVLGVFVGDDLAELGPLPCTVPVASLAPMVAPPRFALARMRVR
                     HVGDPVAFVVAETRAAARDAADAVAVEYRALPSVVDAAAALEATAPQLWPEAHGNLSY
                     RFQKGDADAVQAALAAAAHVVALELVNNRVVISAVETRGAIARYDADGYRLLFSGAGV
                     HALQSQLADSVFRVPRAQMHVACPDVGGGFGVKNALYPEWVMLLWAARRLGRPVAWIG
                     ERAEDFVSTAQGRDNVTRARLALDADGRFLALDVATLANLGAYLSSGGPGSSTNAPAN
                     AIGSGYDIPAICMDVQGVFTNTVPIDAYRGAGKPEVNYMMERLIDAAAQRCGFDRIAL
                     RRRNLVRDFPYRKALGTVIDGGRFAANLDDAVAAADVAGFSERRQASCQRGRLRGIGV
                     TCFMETARGAPNEGAELRFTPDGHVALHVGTQSNGMGHETAYAQIAADLLGLPLETFR
                     YVQADTDAVRAGNGHGGARSMHMGGAALCKAVDTLLEKGRMIAARLLQAAAPQVHFAD
                     GRFVVTHDPARAIGLLQLAEAARDPANLPDGTAPGLDTYVWNLLDVITFPNGCHIAEV
                     EVDTDTGVVTLDRYTAVDDFGTLINPLLTIGQVQGGVAQGIGQAMLEHTVYDPVSGQL
                     LSGSLMDYALPRAADLPDFAITLSGVATQANPLGVKGAGQAGAMAAPQAIVCAVL"
BASE COUNT         1020 a         2242 c         2375 g         1157 t
ORIGIN      
        1 tggatcctgt cccggcgggg cgtgctggac ctggcccgtt cctgggcact gatttcggag
       61 aaccccgccg ccgcagccgg cctgactgac cgaggccgga tcgcccccgg gctgcgtgcc
      121 gatcttgtgg tgatggaacc ggatgcgccc cgcatcgtcg cgacggtcgc ctctggccgc
      181 gtcacctggc ttggggccga gggcgcgtca cggtttcgcg acgaattgat acgcagttga
      241 cacgcattat ccggctggga cacggatcat cggctgaatt ttagtctgtt tgtagaaact
      301 cgcgtctaaa atagcgcaac agactcgatc cggcgccgat tccctcggcg tccccgttgg
      361 agaacaataa gatgaaggtc cggatgcgtc tcgccctgcc gctggtgctg actgtcgccg
      421 gctgtgccac caccaccccg cccctcgcca acagccccgg gccggtcgcc cagaccagcg
      481 cggccccgcc gcaacgggtc agcgcggcgt ccgatgcact cgggcatcag ctcgacagca
      541 tgctggcgaa ctatcgaccc gcctcgacga cgctcacgcg ctgagccggg ttcctgcggc
      601 tgccagaaag gccgggcatg tcccggcctt ttctttgtcc ggtagtgtcc aggtaacctg
      661 ttccaccgca tactgggcgg gtcagcaaac aggttcgggc ccatccgccg ggcgtgaggc
      721 cagttcaggg gcaggccgtc gagaatgccg atccaatgtc ggacaaagac cgagcacagg
      781 aacaacaggc gggcggtcac cgcgttggaa tacgcgctga tcgcggcggc cgtggcattg
      841 gctatcagca gctctgtcat tgtacttgga tcgcagcttg atcgtacctt ggaccatctc
      901 tgggatctga tgcggcaatc ctactcgcac cgcagcggct gacacgcgcg gcgccacgat
      961 caatcggcgg gacagcaaga aggggtcagg ctgacaggcc tgaccccttc acgcgattgg
     1021 ctcccggagt aggactcgaa cctacgaccc agcggttaac agccgcttgc tctaccaact
     1081 gagctatccg ggatcagcgg ggccgttcct atagcgaagc cgatcggcgg acgcaaaccc
     1141 cggcgggccg gaaattccga acaaaatcgc cggcgcagga accgcctaac gcgacacgca
     1201 gaaataacta cgacacactg gtcggcttgt gccacctcgt catggtcggc gaaggccgac
     1261 catccatgac tgtgctgcgg ccccttcgaa gaagtcgtgg atggccagcc tccgccggcc
     1321 atgacggaac ggagcagcgt gccgccagcc aaatcgcgta ttcctgcgtg tcgcctaatg
     1381 cagcgtggca tagcgccgcg ggcgcggccg tgcgccgggc atcatccggg cgtgctccgc
     1441 ctggcaggca cggatcgcct tgcacagcgc gacataggtg cgcgatagcc gcagatggtc
     1501 ggcctcctcg gccagcatat ggagaccctg aagaatcccg atggccgcgt cgtggcaatc
     1561 cgtggcagac ggttcatcat cgtcggtcat caggcggttt gatccccatc ggtaatcaat
     1621 ctcacggggt cgtgagcgaa ggcgcggttc ttatgtcgaa tggctggata gtttgcactg
     1681 cttgaattcc cccctgctgc aactgcaacg gaattagcgg cggaaaagcc acgtaccctc
     1741 ggcattctcg ccggtggcgg tacattgccg gcgcaagtgg cgcgtgccgc ccgcgccgcg
     1801 ggacgggacg ttttcattgt cggcctcgat gggttcgccg aacgagccgt gctggcggag
     1861 tggccgcatg agatcatccg catgggagcg gcgggacgta tcctggcaac gttgcgggcg
     1921 catgactgcc aggacatcgt gctgatcggg ccggtgcgcc ggccgtcgct gctggatatg
     1981 cgccccgatg ccgagggtgc gcgcattctg gcgcgggtcg gcagggcagc gtttgccggg
     2041 gatgacgggt tgctggccgc ggttatccgc gtgctgggcg aggagggctt ccaggtgatt
     2101 ggcgcgcatg aagtgctgcg ggcgccgctg gcgccgggtg gcgtgctgac ccgcgtggcg
     2161 ccggatgcgc aggccctggc ggacatcgcc cgcggcgtgg cggtggtgcg tgcgctgggc
     2221 cgcgtcgacg tcggacaggg ctgcgtggtg cagcaaggca tcgttctggc ggtggaagcg
     2281 gcggagggaa ccgacgccat gctggcacgg gtcgccccgc tgcgccgtga cgggccgggc
     2341 ggcgtgctgg tgaagctggt caagccgggt caggaccgtc gcgccgacct gccgaccgtg
     2401 ggaccgcaaa ccgtgcgttg cgcggctgcg gcaggcttgc gtggggttgc gttcgaggcc
     2461 ggcgggacca ttctggcgga gagcgaagcg accatcgccg ccgcggatgc ggcgggcctg
     2521 tttctgctgg gtctcgatcc cgggaccatt ccagagggga gtgaaggcga atgagccggt
     2581 ttctgcacac catgctgcgc gtcggcgatc tgcagcgcag catcgatttc tacaccaagg
     2641 cgttcggcat gaaggagctg cgccgccgcg acgtgccgga cggcaaatat accctggcct
     2701 tcgtcggctt tggcgacgag gacagcagca cggtcatcga actgacctac aattacggcg
     2761 tggagaaata cgaaatgggc ggcgccttcg gccacctggc gatcggcgtg ccggatgtgg
     2821 cagcggcctg cgaggtcgcg gccaaggcgg gcggcaccat cacgcgcccc gccggcccgg
     2881 tgaagttcgg caccaccatc atcgccttcg tcaaggatcc cgacggctac gcgatcgagc
     2941 tggtgcagcg ggcctgaacc acgacagggg cggcgcccgg ccgcccctgc gccggaatta
     3001 tgccgccttc cgcgcgggtt cctccccgaa cagcttgacc atctcccggc agaacgccgg
     3061 caggtcgtcc ggcttgcgcg aggtgaccag tttaccgtcg cggatcaccg tcttgtcctc
     3121 ccaggttgca cccgcattgc gcaggtcggt ccgcagcgag ggccaggacg tcatggtgcg
     3181 gccgcgcacg ccttcggctt cgatcagcgt ccacggtccg tggcagatcg ccgcgatcgg
     3241 cttgcccgat tgcacgaagt gccgcaccaa cgccaccgcc tggggaatgg tgcgcagcat
     3301 gtcggggttc gccacgccgc ccggcagcac cagcccgtcg aaacgttcgg gggaagcctt
     3361 gtcgagttcc tggtccaccg gcagcttgtc ggccttgtcg tggtggttca tgccctggat
     3421 ctgcccgcca tgcggcgcga tcaccaccac ctccgccccg gcctgcttca gcgccttgac
     3481 cggttcggtc agttccacct gctcaacgcc gtcggtcgcc agcacggcga ttttgcgccc
     3541 gctgatatcc gccatatgca gtctcctgtt gtctgagaga ggattcaact ggccggaccc
     3601 ggtccgggtt gcgctgccct tctggaagga gccctgatgc ggacgcatag ggtggagcgc
     3661 ctgctaccct acgccagcgc gccgctcggc cggttccgca gccatcgcca gatcggcttc
     3721 gcctggcccg agggagcggt tcggctcggg ccgcgcatcg ccctggtctg ccattacgat
     3781 gccggcggac ggctgcggcc tgacctgctg gcctatctgc gggcgctgcg cgacgccggg
     3841 ttctccgtcg tgctggtgtc gaacagcgga acgatgcatc cggatgcgct gcaagcgggc
     3901 cgcgcgctgt gcgcggcggt gctggtgcgg cgcaatctcg gcctggattt ctgcgcctgg
     3961 cgcgacgcga tggaatggct cgggctgccg catgccggca cggcgcagtt gctgctggcc
     4021 aacgacagcg tctacggccc gctgcgcccg ctggcgccgc tgctcgcgcg gatcggcgat
     4081 gcgcccggcc tttggggcat gaccgacagc gtggagcgtg ggttccatct gcaatcctat
     4141 ttcctgctcg cgcaggccga cgtgatccgc agcgcagcct ggcagcgctt ctggcgcggc
     4201 gttcggccgc tgcggtcgaa atggctgatg atcgggctgt atgaggtggg gctgacgcag
     4261 cggttgctgc gcacggggct cggatgccat gcgttgttca gcggcgtcgc gggggacggc
     4321 aagctgttcg ccaatccaac attggatggc tggcgcatcc tgctggacgc cgggtttccc
     4381 ttcctcaagc gcgagttgct ccgcgacaat ccgaccctgg tgccggatct cgcggcctgg
     4441 cggacacgcg tcaccgagat cgccggtgcg cgcgcggtcg cctggatcga ggcggatctc
     4501 gccggccgcc gaacgcaaac cggcctgtcg aattccgcag ccgccctgta gcatggcggc
     4561 ctgaaccgca gcatcagagc cgccatgccc tatcgaggcc aatcccttcg ccgcatcgaa
     4621 gacgcgcgct tcctgaccgg cggcgcacgt tatgtcgaag acattgtggc ggccggcgat
     4681 gcctggatgc aggtggttcg ctcgccgcat gcgcatgccg aactgctgcg gatcgacacg
     4741 accgcggccg cggcgatgcc tggcgtgctg ggcgtgttcg tgggggacga cctggcggaa
     4801 cttgggccac tgccgtgcac ggtaccggtc gccagcctcg cgccgatggt tgcaccgccg
     4861 cgcttcgccc tggcacgcat gcgggtgcgg catgtcggcg atccggttgc tttcgtggtg
     4921 gcggagaccc gcgctgccgc gcgcgacgcc gccgacgcag tcgcggtgga gtaccgcgcg
     4981 ctgccctcgg tggtcgatgc cgccgccgcg ctggaagcaa ctgccccgca actctggccc
     5041 gaggcgcacg gcaatctgtc ctaccgcttc cagaagggcg atgcggatgc ggtgcaggcg
     5101 gcgcttgccg cggcggcgca tgtggtcgcg ctggaactgg tgaacaaccg cgtcgtcatc
     5161 agcgcggtgg aaacccgcgg cgcgatcgcc cgctacgatg cggatggcta tcgcctgctg
     5221 ttctccggtg ccggcgtgca cgcactgcag tcgcaactgg ccgacagcgt gttccgtgtg
     5281 ccgcgcgcgc agatgcatgt cgcctgtccc gatgtgggtg gcgggttcgg cgtgaagaac
     5341 gcgctttatc ccgaatgggt catgctgctg tgggcggcgc gccggctggg ccgtccggtg
     5401 gcgtggattg gcgagcgggc cgaggacttc gtctccaccg cacagggccg cgacaatgtc
     5461 acccgcgcgc ggctggcact cgatgcggac ggccgcttcc tggcgctgga tgtcgccacg
     5521 ctggccaatc ttggcgcgta tctgtcctcc ggcggtcccg gcagttcgac caacgcgccg
     5581 gccaatgcca tcggcagcgg ctacgacatt ccggcgatct gcatggatgt gcagggtgtc
     5641 ttcaccaaca ccgtgccgat cgacgcgtat cgcggcgccg gcaagccgga agtgaactac
     5701 atgatggagc ggctgatcga cgccgccgcg caacgctgcg gcttcgaccg gatcgcgctg
     5761 cgccgccgca acctggtgcg cgactttccc tatcgcaagg cgctcggcac ggtgatcgac
     5821 ggcggccggt ttgctgcgaa cctggacgat gcggtggcgg ccgcggatgt cgccggattt
     5881 tccgagcgcc ggcaggcatc gtgccagcgc ggccggctgc gcgggatcgg cgtgacctgc
     5941 ttcatggaaa ccgcgcgcgg cgcgccgaat gaaggcgcgg aactgcgctt cacgccggac
     6001 ggccatgtcg cgctgcatgt cggcacgcaa tccaatggca tgggccacga gaccgcctat
     6061 gcgcagattg ccgccgatct gctcggcctg ccgctggaga cattccgcta cgtgcaggct
     6121 gacaccgacg cggtgcgtgc cggcaacggg catggcggtg cgcgctccat gcacatgggc
     6181 ggtgccgcct tgtgcaaggc ggtggacacg ctgctggaga agggccgcat gatcgccgcg
     6241 cggctgctgc aggcggcagc gccgcaagtg cactttgccg atggccgctt cgtggtgacg
     6301 cacgatccgg cgcgcgcgat cggcctgctg cagctcgccg aggccgcacg cgatcccgcc
     6361 aacctgccgg acggtacggc gcccggcctg gatacctatg tctggaacct gctggacgtc
     6421 atcaccttcc cgaacggctg ccacatcgcg gaagtggagg tggacacgga taccggcgtg
     6481 gtgacgctgg accgctacac ggcggtggac gatttcggca cgctgatcaa tccgctgctg
     6541 accatcggcc aggtacaggg cggcgtggcg cagggcatcg gccaggcgat gctggagcac
     6601 acggtgtatg atccggtgtc aggccagctg ctcagtggct cgctgatgga ctacgcgctg
     6661 ccgcgcgccg ccgacctgcc ggatttcgcc atcacgctga gtggggttgc cacccaggcc
     6721 aatccgctgg gggtgaaggg ggccgggcag gcgggcgcca tggccgcgcc gcaggcgatc
     6781 gtctgcgcag tgct
//
LOCUS       DASSZH010000100         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_53871, whole genome shotgun sequence.
ACCESSION   DASSZH010000100 DASSZH010000000
VERSION     DASSZH010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53871"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VFN42_01975"
     CDS             complement(<1..729)
                     /locus_tag="VFN42_01975"
                     /inference="COORDINATES: protein motif:HMM:NF015699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HET8995414.1"
                     /translation="MNAPLASMTGFAHAQGAVGGLAWTWELRSVNGRGLDLRFRLPPG
                     WDALEPALRESAGKALRRGNVTANLAVKRDSEARLALDSAALEQALTLAMDLHARLPG
                     SPVPRAEALLSLPGILRPAAADPVEERATAGPAVQAGFTEALHGLVAARHAEGARLAT
                     TLTNLLDEIEALRSQAAEEAADQPAAQRARVMENLRALLRETPSLPEERIAQEVALLA
                     ARSDVREELDRLHSHVQAAHALMRE"
     gene            complement(777..>2238)
                     /locus_tag="VFN42_01980"
     CDS             complement(777..>2238)
                     /locus_tag="VFN42_01980"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET8995415.1"
                     /translation="EVFVTDDGAETDLDLGHYERFTGVHATQLDNATTGRIYQDVIAR
                     ERRGDYLGATVQVIPHITDAIKEVIVRNTAEFDFVLVEIGGTVGDIESLPFLEAIRQL
                     GNELGRERAMFVHLTLVPYIPSAGELKTKPTQHSVKELQNVGIQPQMLLCRCDRPIPD
                     NERHKIALFCNVRPEAVIAALDVDTIYAVPISYHREGMDREVLRHFDMPFDREPDLTR
                     WGEIVEAIRAPEGEVRIAVVGKYINLLDSYKSLAEALAHGGIANRVKVKLDWLDSEVF
                     EQPGTVHRLEGVHGILVPGGFGERGTPGKIEAVRFARERGVPFFGICFGMQMAVIEAA
                     RNLVGLPEASSTEFGPCDTPVVGLLTEWARGNEIERRMEGGNLGGTMRVGAYPAVLTP
                     GSLVRDLYGGAARIEERHRHRYEVNVHYKKRLEAAGMRFSGMSPDGVLPEIIELPDHP
                     WFVGVQYHPELKSKPFEPHPLFAAFIEAAVRQARLV"
BASE COUNT          336 a          774 c          765 g          363 t
ORIGIN      
        1 ctcgcgcatc agcgcgtgcg ccgcctggac atggctgtgc agccggtcca gctcctcgcg
       61 cacgtcggac cgcgccgcga gcagcgcgac ttcctgggcg atgcgttcct cgggcaggga
      121 cggggtttcg cgcagcaggg cgcgcaaatt ctccatgacc cgggcgcgct gtgcggccgg
      181 ctggtccgcg gcttcctcgg ccgcctgact gcgcagggct tcgatctcgt cgagcaggtt
      241 ggtcagcgtg gttgccagcc gtgcgccttc agcgtggcgg gcggccacga gaccgtgcaa
      301 cgcttcggtg aagccggcct gcaccgcggg gccggcggtg gcgcgctctt ccaccgggtc
      361 ggcggcggcg gggcgcagga tgccgggcag cgacagcaac gcctcggcgc gcggcaccgg
      421 gctgccggga agccgggcat gcagatccat cgccagggtc agcgcctgtt ccagtgcggc
      481 cgaatccagc gcgagccgag cctcggagtc gcgcttgacc gccaggttgg cggtgacgtt
      541 gccgcgccgc agcgccttgc cggcggattc gcgcagcgcg ggttccagcg catcccagcc
      601 gggcggcaag cggaagcgca gatccaggcc gcggccgttc acgctgcgca gttcccaggt
      661 ccaggcgagg ccgcccaccg cgccttgcgc gtgggcgaag ccggtcatcg aggcgagagg
      721 cgcgttcatg ccgcggcttg tatcagccgg ccgggggcct gccaaacacc aggccgctac
      781 accagccgcg cctgccgtac cgctgcctcg atgaaggcgg cgaacagcgg gtggggctcg
      841 aagggcttgg atttcagctc cgggtggtac tgcacgccga cgaaccaggg atggtccggc
      901 agttcgatga tctccggcaa gacgccgtcg ggcgacatgc cggagaagcg catgccggcg
      961 gcttccaacc gtttcttgta gtgcacgttg acttcgtagc ggtggcggtg gcgttcctcg
     1021 atgcgcgccg cgccgccgta caggtcgcgg accagcgagc cgggcgtcag caccgcggga
     1081 taggcaccca cccgcatggt gccgccgagg ttgccgcctt ccatgcgccg ttcgatttcg
     1141 ttgccgcggg cccactcggt cagcaatccc accaccggcg tgtcgcaggg accgaactcg
     1201 gtggacgagg cttcgggcag gcccaccagg ttccgcgccg cctcgatcac cgccatctgc
     1261 atgccgaagc agatgccgaa gaacggcacg ccgcgttccc gggcgaaacg caccgcctcg
     1321 atcttgcccg gcgtgccgcg ctcaccaaag ccgccgggaa cgaggatacc gtgcaccccc
     1381 tccagccgat gcaccgtgcc gggctgctcg aacacctcgc tgtccagcca gtccagcttc
     1441 accttcaccc gattggcgat gccgccatgc gccagcgcct cggccagcga cttataactg
     1501 tccagcaggt tgatgtattt cccgaccacg gcgatgcgca cctcgccttc cggcgcgcga
     1561 atcgcctcga cgatttcgcc ccaccgcgtc aggtccggct cgcggtcgaa cggcatgtcg
     1621 aaatgtcgca gcacttcgcg gtccatgcct tcgcggtgat agctgatcgg cacggcatag
     1681 atcgtatcga cgtccagggc ggcgatgacc gcctcgggcc gcacgttgca gaacagcgcg
     1741 atcttgtggc gctcgttgtc gggaatggga cggtcgcagc ggcacagcag catctgcggc
     1801 tggataccga cgttctgcag ctccttcacc gaatgctgcg tcggcttggt cttcagctca
     1861 ccggcggacg ggatgtacgg caccagggtc aagtgcacga acatcgctcg ctcgcgcccc
     1921 aactcgttgc cgagctggcg gatggcctcc aggaagggga gggattcgat gtcgccgacg
     1981 gtgccgccga tctccaccag cacgaagtcg aactccgcgg tgttgcggac gatcacttcc
     2041 ttgatggcat cggtgatgtg cgggatcacc tgcacggtgg cgcccaggta gtcgccgcgg
     2101 cgttcccgcg cgatcacgtc ctgatagata cggccggtgg tggcgttgtc cagctgtgtc
     2161 gcatgcacgc cggtgaagcg ttcataatgg cccagatcca ggtcggtctc ggcaccgtcg
     2221 tcggtgacga acacctcc
//
LOCUS       DASSZH010000101         6672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1690316, whole genome shotgun sequence.
ACCESSION   DASSZH010000101 DASSZH010000000
VERSION     DASSZH010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6672
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690316"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /gene="galE"
                     /locus_tag="VFN42_01985"
     CDS             <1..690
                     /gene="galE"
                     /locus_tag="VFN42_01985"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007282930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET8995416.1"
                     /translation="RLIEACVRHGVRRFVLSSTAALFGTPDQVPIPETARIDPGSPYG
                     ESKWMIERALLWADRIHGLRSACLRYFNAAGADPDGRAGEDHNPETHLIPLVIDAALG
                     RRMGLEVYGDDYPTPDGTCIRDYVHVADLAAAHLLAVDRLARGSLTWNLGNGQGHSVL
                     EVIGAVERVSGRRVPYHMAPRRPGDPAALVAGSAAAQAAGGQPRFGALDAIVRTALAW
                     REAHPDGYAEG"
     gene            complement(697..1704)
                     /locus_tag="VFN42_01990"
     CDS             complement(697..1704)
                     /locus_tag="VFN42_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006558315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HET8995417.1"
                     /translation="MATRSVAAVFGGSGFIGRYVVKRLAQRGFIVRAAMRDTEAGRFL
                     KPMGAVGQIVPLYASVTNDAAVHRVVEGASLVVNLVGILSESRKGEFQHVHAEGAGRV
                     ARLAAAAGVQRMVHVSAIGADPASPSEYGRSKAAGEQAVHAAFPGATIMRPSIVFGQE
                     DQFFNRFGAMARLLPCMPVIAGQTRLQPVYVADVADAIMAALGRADAAGVTYELGGPR
                     VWTMRELLAYVLKETGRRRRLIELPMGLARLQASLMELLPGKPLTRDQLLMLARDNVV
                     AEGMPGLPELGIVPSPVELIVPAYLRRFQPGGGRRPVLPDQQIGGQTDLSFQTRDVGA
                     G"
     gene            complement(2014..2709)
                     /locus_tag="VFN42_01995"
     CDS             complement(2014..2709)
                     /locus_tag="VFN42_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995418.1"
                     /translation="MSFTTLCLSVLALSPELAATLISGLLAAVPVVAGETDPQVRQAT
                     LIREMNELHPRDLEEAILASQVLAAHHNAQACSTALAGLDPASKEASRLRRDVVAMQR
                     LVLATQRAFHQSQARPMQQDAEGGDPRPMVPARQANPPRGRAAAKEIPPMPAGAAAAE
                     QPGAAVAAGARPKFDPFKADPSLARLRERWNTVGCWEDMTMEERRETFGYSYTPKGKT
                     AASGSTEVPRPAA"
     gene            complement(3246..3815)
                     /locus_tag="VFN42_02000"
     CDS             complement(3246..3815)
                     /locus_tag="VFN42_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET8995419.1"
                     /translation="MPASLLLALRAMAYNNAWANHRLLTACAGLSQAEFAAPRVGFFP
                     SLQATLNHILTVDLFYIDALEGGTLGPAAWADPVPCKTVAELHPAQAALDRRLIAWCN
                     ALPPDGPDRIVAVHRGTRIQRERADRLLLHLFQHQVHHRGQAHAMLAGTSVKPPQLDE
                     FFSVGEAPLRAEEFTALGWTEAAIWDASS"
     gene            complement(3898..4239)
                     /locus_tag="VFN42_02005"
     CDS             complement(3898..4239)
                     /locus_tag="VFN42_02005"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET8995420.1"
                     /translation="MAEVELAPEVLDDFDRILEHLARFEVADAQARITGMIEAVQVLT
                     HSPLIGRPVRGGKRELVIGRGSHGYLALYRFVASIDTVFVLAIRSQREQDDKQQGSRH
                     VADSRGGRMQA"
     gene            complement(4248..4529)
                     /locus_tag="VFN42_02010"
     CDS             complement(4248..4529)
                     /locus_tag="VFN42_02010"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HET8995421.1"
                     /translation="MSTTTIRLEDALKARVAAAAERAGKTTHAFIRDAIAQTVEQAEL
                     EDALHRVADERWAAMLATGETVGWDDARAWLTARSRGERPARPKSRKPG"
     gene            4782..6026
                     /locus_tag="VFN42_02015"
     CDS             4782..6026
                     /locus_tag="VFN42_02015"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET8995422.1"
                     /translation="MARIVMKFGGTSVADLDRIRNVAQRVKREVDAGNQVAVVVSAMS
                     GVTNQLVAWCQALSPLHDAREYDTVVATGEQVTTGLLSIALQEIGVEARSWQGWQIPI
                     RTDDAHGKARIQDIGGSDIIARMQGSAQRAPQVAVVAGFQGIGPDGRITTLGRGGSDT
                     SAVALAAALKAERCDIYTDVDGVYTTDPRIVPQARRLPRIAYEEMLELASVGAKVLQT
                     RSVELAMNERVRVRVLSSFKDAADDNDGTLVVDEDEIVEKQNVAGIAYSRDEAKITVR
                     RVPDRPGVAASIFGALAAQNANVDMIVQNISADGTTDMTFTVGKADLPRARLALDAVK
                     QQIGFQEVLEDPNVAKISVVGVGMRSHAGVANTMFQALADKAINIQVISTSEIKVSVL
                     IAAEYTELAVRALHTAYGLDAA"
     gene            6016..>6672
                     /locus_tag="VFN42_02020"
     CDS             6016..>6672
                     /locus_tag="VFN42_02020"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014104560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HET8995423.1"
                     /translation="MQPDSATARAELDRLWARGTAFLGCRVALMGGAMSWVSERHLVS
                     AISNAGGFGVIACGSMNPDQLAAEIAGTQALTSRPFGVNLITMHPQLDDLIRVCLAAK
                     VGHIVLAGGIPPGAAVRAVKDGGAKLIAFTPALVLAKRLVRGGADALVIEGSEAGGHI
                     GPVSLNVLAQEILPHIREVPVFVAGGIGRGEAILAYLEMGAAGAQLGTRFAAAKESIA
                     H"
BASE COUNT          985 a         2348 c         2339 g         1000 t
ORIGIN      
        1 cgcctgatcg aggcctgcgt gcggcacggg gtgcgccgct tcgtgctgtc ctccaccgcg
       61 gcgctgttcg gcacgccgga ccaggtgccg atcccggaga ccgcacggat cgaccctggc
      121 tccccctatg gcgagagcaa gtggatgatc gagcgcgccc tgctgtgggc cgaccggatc
      181 cacgggctgc gcagcgcctg cctgcgctat ttcaacgcgg ccggcgccga tccggacggc
      241 agggcgggcg aggaccacaa cccggaaacc cacctgattc ccctggtcat cgatgccgcg
      301 ctcggccggc ggatggggct ggaggtctat ggcgacgact atcccacgcc ggacggcacc
      361 tgcatccgcg actacgtgca cgtcgccgac ctggcggcgg cgcacctgct ggcggtggac
      421 cggctggcgc gcggcagcct gacctggaac ctgggcaacg gccaggggca ttcggtgctg
      481 gaggtgattg gcgcggtgga gcgggtgtcc ggccgccgcg tcccctatca catggcgccg
      541 cggcggcccg gcgacccggc cgcgctggtc gccggttcgg cggcggcaca ggcggccggc
      601 gggcagccgc gcttcggcgc gctggacgcg atcgtgcgca ccgccctggc ctggcgggag
      661 gcgcatccgg atggctacgc cgagggctga gcccggctag ccggcgccga cgtcgcgggt
      721 ctggaaggac aggtcggtct ggccgccgat ctgctggtcc ggcagcaccg ggcggcgccc
      781 gccgcccggc tggaaccgcc gcagataggc cggcacgatc agctcgaccg ggctgggcac
      841 gatgcccagt tccggcaggc cgggcatgcc ctcggccacc acgttgtcgc gcgccagcat
      901 cagcaactgg tcgcgggtca gcggcttgcc gggcagcagc tccatcaggc tggcctgcag
      961 ccgagcgagg cccatcggca gctcgatcaa ccgccggcgc cgcccggtct ccttcagcac
     1021 gtaggcgagc agctcgcgca tagtccagac ccgcgggccg cccagctcgt aggtgacgcc
     1081 cgcggcatcg gcgcggccca gcgccgccat gatcgcgtcg gcgacgtccg cgacatagac
     1141 tggctgcagc cgcgtctggc cggcgatcac cggcatgcag gggagcaggc gcgccatcgc
     1201 gccgaaccgg ttgaagaact ggtcttcctg cccgaacacg atcgagggcc gcatgatggt
     1261 ggcgccgggg aacgccgcgt gcaccgcctg ctcgccggcg gccttgctgc ggccgtattc
     1321 gctcgggctg gccgggtcgg cgccgatcgc cgagacatgc accatccgct gcaccccggc
     1381 cgccgccgcc agccgggcga cccggccggc gccctcggca tgcacgtgct ggaactcgcc
     1441 cttgcggctc tccgagagga tgccgaccag gttcaccacc agggaggcgc cctccaccac
     1501 gcgatgcacg gcggcgtcgt tggtcaccga ggcgtacagc ggcacgatct gtcccaccgc
     1561 gcccatgggc ttgaggaagc ggccggcctc cgtgtcgcgc atggccgcgc gcacaatgaa
     1621 gccacgctgc gccaggcgct tgaccacgta gcgcccgatg aagcccgagc cgccgaatac
     1681 cgccgccacg ctgcgcgtcg ccattgagac tgtctcctag cacttgcgtg cggcgggttt
     1741 tgtcaccaaa gcggtccgaa taccaccggc aacccgacca caccgctttg ccgccggtgt
     1801 gtgcaccagc ggacggtcgg tctcaagggc ggggtggcgg agggcatgcc gggcgcaggc
     1861 attgcccagg acgttgcgcg cacgcacgcg ccggcgtcat tgcccgcgca ggcatgcccg
     1921 ggcgtcatga tccgcaaagg cgcgccccgg cgtcatggtc cgcgcaggcg gaccatccac
     1981 gacttgcggg cgtggttcag gaaaaaccca ggatcaggcc gcggggcggg gcacctccgt
     2041 gctgccggaa gccgccgtct tgcctttcgg cgtgtagctg tagccgaagg tttcccgccg
     2101 ttcctccatg gtcatgtctt cccagcaccc gaccgtgttc cacctctcgc gcaaccgtgc
     2161 gagcgaggga tccgccttga agggatcgaa ctttggccgc gcgcccgccg ccactgccgc
     2221 gcccggctgt tccgccgctg ccgcccccgc gggcataggc ggaatttcct tggccgccgc
     2281 cctgccgcgg ggcgggttgg cctgccgggc cggcaccatg ggacgcgggt cgccgccttc
     2341 cgcgtcctgc tgcatcggcc gagcctggct ttggtggaag gcccgctggg tggcgaggac
     2401 gaggcgctgc atcgccacga cgtcgcgccg cagtcgggag gcttccttgc tggcgggatc
     2461 cagcccggcc agcgctgtgc tgcacgcctg cgcgttgtga tgcgcggcga ggacctgcga
     2521 agccaggatg gcttcttcca ggtcgcgcgg gtggagttcg ttcatctccc ggatcagcgt
     2581 ggcctgccgc acctggggat cggtttcgcc ggccaccacc gggacggcgg cgagcaggcc
     2641 gctgatcagg gtggcggcga gttccgggct gagggcgagg acgctgaggc agagagtggt
     2701 gaacgacatt gcgggccggc tcctttttcc ggctgggaaa aacgcgcgaa caatagcaga
     2761 acttaggaga gaagtcaagg cgtattttcc ttggaaggtt gccggtacgg catgcggggc
     2821 cgccgcgccg gtcaagcgat ggttcggcgc ccgaccttga gggccaaggg gatcggatga
     2881 cctacctgcg aatgatgcgc ggggtccgtt tcggactttc caggagctgt cgtttgcggg
     2941 tgaggacgag acgcaggtgt cggacgaata ctgcttgtcg cgggttcaat cctgatccaa
     3001 cgggaagctg tcagggcgat tcatcggctt catcacgccc ggtgcatgaa cgacctgcgg
     3061 gcagccggtt tgcaccggtg cctgctgatg aacgtcggca caccgcgcct gggaatccgg
     3121 cgcgtcctcc tggcgctccg cagaacgctc cgcggcgggc cgcccccacg cactcttcgc
     3181 gttcatctgc gcagcggctt attggcgttt ccctgcctgg cttgcaggcg atccttgatc
     3241 cgctctcacg acgacgcgtc ccagatcgcc gcctccgtcc agccgagcgc cgtgaattcc
     3301 tcggcgcgca ggggcgcttc gccgaccgag aagaattcgt cgagctgcgg cggcttcacg
     3361 gacgtaccgg ccagcatggc atgcgcctgg ccgcgatggt gcacctggtg ctggaacagg
     3421 tgcagcagca agcggtcggc acgctcgcgc tggatccgcg tgccgcggtg caccgccacg
     3481 atgcggtcgg gtccgtccgg cggcagcgcg ttgcaccagg cgatcagccg gcggtccagc
     3541 gcggcctgcg ccgggtgcaa ctcggccacc gtcttgcagg gaaccggatc ggcccaggcg
     3601 gcggggccga gcgtgccgcc ttccagcgcg tcgatgtaga acaggtccac cgtcaggatg
     3661 tggttcagcg tcgcctggag ggacggaaag aaaccgaccc gcggcgccgc gaattccgcc
     3721 tggctcagcc cggcgcaggc ggtcagcagg cggtgattgg cccaggcgtt gttgtacgcc
     3781 atggcgcgca gggcgagcag cagcgaagcc ggcatgggaa ccctccggtt ggcgcgccgg
     3841 ttctggccgg cacacgcgca gtgtgacagg acccggccgg aagcatgtgc cgctgcttta
     3901 tgcttgcatc cgtccgcccc ggctatcggc aacgtgccgc gagccctgct gcttgtcgtc
     3961 ctgttcccgc tggctgcgga tcgccagcac gaacaccgtg tcgatgctgg ccacgaaccg
     4021 atacagcgca agatatccgt gcgaaccccg cccgatcacc agctcgcgct tgccgcccct
     4081 gaccgggcgg ccgatcagcg gtgagtgggt caggacctgc accgcctcaa tcatcccggt
     4141 gatgcgggcc tgggcgtcgg cgacctcaaa ccgcgccaga tgctccagga tccggtcgaa
     4201 atcgtcgagc acttccgggg cgagttcgac ctcggccacg cagccggtca gccgggcttg
     4261 cgggatttcg gccgagccgg ccgttcgccg cgcgaccgcg ccgtgagcca ggccctggca
     4321 tcgtcccaac ccactgtctc gccggtcgcc agcatggccg cccaccgctc gtccgccacg
     4381 cgatgcaacg cgtcctccag ttccgcctgc tcgaccgtct gggcgatggc gtcgcggatg
     4441 aaggcgtgcg tcgtcttgcc ggcccgttcg gccgccgcgg cgacgcgggc cttcagcgcg
     4501 tcctccaggc ggatggtcgt cgtgctcatg ccatgcctca caacagaagg cggatgtagc
     4561 acaatcgtgc tactccggca aacgcccctc cgggatcagc gctgccaccc tgaacccaca
     4621 cgtgacctgc ggaaccgaac ccccggggcc gcacgccggc cggccccgtc ccctgattcg
     4681 tgcccggcgc agcagggcag accggcaaca acgcccggtt gcccctccgc cgcggcgcca
     4741 gtatgtgtcc ccctcccgtt ccctattcgc ccagcaggtt catggcgcgc atcgtaatga
     4801 agttcggcgg cacctcggtg gccgacctcg accgcatccg caacgtcgcc cagcgggtga
     4861 agcgggaggt tgacgccggc aaccaggtcg ccgtcgtggt ctccgccatg tccggcgtca
     4921 ccaaccagct tgtcgcctgg tgccaggcgc tgtcgccgct gcacgacgcg cgcgaatacg
     4981 acaccgtggt cgccaccggc gagcaggtca ccaccgggct gctgtccatc gcgctgcagg
     5041 agatcggtgt agaggcgcgg tcctggcagg gctggcagat ccccatccgc accgacgacg
     5101 cgcacggcaa ggcgcgcatc caggacatcg gcggcagcga catcatcgcc cgcatgcagg
     5161 gcagcgccca gcgcgcgccg caggtcgcgg tggtcgccgg gttccagggt atcggcccgg
     5221 acggccgcat caccacgctc gggcgtggcg gatcggatac ctccgccgtg gcgctggctg
     5281 cggcgctgaa ggcagagcgg tgcgatatct ataccgacgt ggacggcgtc tatacgaccg
     5341 acccgcgcat cgtgccgcag gcgcgccggc tgccgcgcat cgcctatgag gagatgctgg
     5401 aactcgcctc ggtcggcgcc aaggtgctgc agacccgcag cgtcgagctg gcgatgaacg
     5461 agcgggtgcg cgtgcgcgtg ctgtccagct tcaaggacgc ggcggacgac aatgacggca
     5521 cgctggtggt ggacgaggac gagatcgtgg aaaagcagaa cgtagccggc atcgcctatt
     5581 cgcgcgacga ggcgaagatc accgtgcgcc gggtgccgga ccggcccggg gtcgccgcca
     5641 gcatcttcgg cgcgctggcg gcgcagaatg ccaatgtcga catgatcgtg cagaacatct
     5701 ccgccgacgg caccaccgac atgaccttca cggtgggcaa ggcagacctg ccgcgcgccc
     5761 gcctggcgct ggatgcggtg aagcagcaga tcggcttcca ggaggtgctg gaggatccca
     5821 acgtcgccaa gatcagcgtg gtgggggtcg gcatgcgcag ccatgccggc gtggccaaca
     5881 cgatgtttca ggcgctggcc gacaaggcga tcaacatcca ggtgatctcc accagcgaga
     5941 tcaaggtctc cgtcctcatc gccgccgagt acaccgaact ggcggtgcgg gcgctgcata
     6001 cggcgtacgg gctcgatgca gcctgacagc gccaccgccc gcgcggaatt ggaccgcctg
     6061 tgggcacgcg gtaccgcttt cctcggctgc cgcgtcgcgc tgatgggcgg tgcgatgagc
     6121 tgggtcagcg aacgccacct ggtctccgcc atctccaatg ccggcggctt cggggtgatc
     6181 gcctgcggct cgatgaaccc ggaccagctg gcggcagaga tcgccggcac ccaggcactg
     6241 accagccggc cgttcggcgt gaacctgatc accatgcacc cgcagctcga cgacctgatc
     6301 cgggtctgcc tggcggcgaa ggtcggccat atcgtgctgg ccggcggcat cccgcccggc
     6361 gccgcggtgc gcgcggtgaa ggacggcggc gccaagctga tcgccttcac cccggcgctg
     6421 gtgctggcca aacgattggt ccgcggcggc gccgacgcat tggtcatcga gggatcggaa
     6481 gccggcggcc atatcggtcc ggtgtcgctc aatgtcctgg cgcaggagat cctgccgcat
     6541 atccgcgagg ttccggtgtt cgttgccggc ggcatcggcc gcggcgaggc gatccttgcc
     6601 tacctggaaa tgggcgccgc cggcgcgcag ctcggcaccc gcttcgcggc ggcgaaggaa
     6661 tcgatcgcac ac
//
LOCUS       DASSZH010000102         2248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_837788, whole genome shotgun sequence.
ACCESSION   DASSZH010000102 DASSZH010000000
VERSION     DASSZH010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837788"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="VFN42_02025"
     CDS             complement(<1..779)
                     /locus_tag="VFN42_02025"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8995424.1"
                     /translation="MPQLPPGLDTDIVVIGAGLAGLGAASLLRDQGLRCVVLEASGRI
                     GGRAWTAYPEALGGAWFDMGAVWLHSAEHNPLVPIAQAAGDTLLRSDELRREHTMIGT
                     REATPAEYADYDQAWCRFEATADRLLRNVPDTALAHVAQHLPGDPWALTVESFEGPVI
                     CAVAADKFSLRDWRDNVLSGSNLVPQGGIGAFVQRRLGQGLDIRLNTPATRVRWNGPG
                     GRVAVETGGGTITAGACIVTVSTGVLGAGALAFDPALPAGT"
     gene            complement(844..1896)
                     /locus_tag="VFN42_02030"
     CDS             complement(844..1896)
                     /locus_tag="VFN42_02030"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET8995425.1"
                     /translation="MSTQQWQLNQSQLRTLVVLAAAAVGFYFCFALARPFLSPLTMGL
                     VLGLLLLPLHRRIERSLPWPSAAAFVSVAVAGVVIIVPGLFVAHQLGREAAIGAGYLE
                     HLLRDTEWRDAAAGYPRIAEVVVWIEQRLNPAGMLADLASWLTSRSTLLLRGSVTQLI
                     NGILTFYLLFYVLRDRHQARRLLASISPLSDAETDELIQRFTDTVHATVFGTVVVAGV
                     QGALGGLMFWWLGLPSPMLWALVMALLAVVPVLGTFVVWVPAAIFLALSGAWVKAVIL
                     VAWGGGLVATIDNLLYPILVGNRLRLHPVPTFIGMIGGMFLFGTSGLVLGPAIIAVTQ
                     ALLNILRRRFESAPLR"
     gene            complement(2109..>2248)
                     /locus_tag="VFN42_02035"
     CDS             complement(2109..>2248)
                     /locus_tag="VFN42_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246983.1"
                     /note="metalloprotease; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HET8995426.1"
                     /translation="TAHLFIVNPLSGAHLGSLFSTHPPIEERIARLSAMAQQRSPWTV
                     R"
BASE COUNT          385 a          790 c          770 g          303 t
ORIGIN      
        1 tgcgttccgg ccggcaaggc cgggtcgaac gccagggcgc cggcccccag caccccggtg
       61 gagacggtga cgatgcacgc gccggcggtg atcgtgccgc cgccggtctc gacggcgacc
      121 cgtccccccg gcccgttcca ccgcacccgc gtcgccggtg tgttcaaccg gatatccagc
      181 ccctgcccca gccgccgctg cacgaaggcg ccgatgccgc cctgcggcac cagattgctg
      241 ccggacagca cattgtcccg ccaatcgcgc aggctgaact tgtccgccgc cacggcgcag
      301 atcacaggtc cctcgaagct ctccaccgtc agtgcccagg gatcgccggg caaatgctgg
      361 gccacatgcg ccagcgcggt atcaggtacg ttccgcaaca gccggtcggc ggttgcctcg
      421 aagcggcacc aagcctggtc gtagtcggcg tattccgccg gcgtggcctc gcgcgtgccg
      481 atcatggtgt gctcacgccg caattcgtcg gagcgcagca gcgtgtcgcc ggcggcctgg
      541 gcgatcggca ccagcgggtt gtgttccgcg ctgtgcagcc acaccgcgcc catgtcgaac
      601 caggcgccgc cgagcgcttc cggataggcc gtccaggcgc ggccaccgat gcggccggac
      661 gcctctagca ccacgcagcg cagcccctgg tcgcgcagca ggctggcggc cccaagtccc
      721 gccagcccgg cgccgatcac cacgatgtcg gtgtccagcc cggggggaag ttgcggcatg
      781 cggctctcct ggcgatatgg acacaaggtt ggctgctcgc cgccgggcgg caaggtgccc
      841 ggttcaacgc agcggcgcgc tttcgaatcg gcgccggagg atgttcagca aggcctgggt
      901 gacggcgatg atcgccggtc ccagcaccag cccggatgtg ccgaacagga acatgccgcc
      961 gatcatgccg atgaaggtcg gcaccgggtg cagtcgcaac cggttgccga ccaggatggg
     1021 gtacagcagg ttgtcgatcg tggcgaccag tccgccgccc cacgcgacca ggatgacggc
     1081 cttcacccag gcaccactca atgcgagaaa gatggcggcc ggcacccaaa ccacgaaggt
     1141 gccaagcacc gggacgaccg ccagcagcgc cattaccagc gcccacagca tcggcgacgg
     1201 caggccgagc caccagaaca tcagcccgcc cagcgccccc tgcacgccgg cgacgaccac
     1261 ggtaccgaac acggtcgcgt gcacggtatc ggtgaagcgc tggatgagct cgtcggtctc
     1321 cgcgtcgctc agcggcgaga tcgaggccag cagccgccgc gcctgatgcc ggtcgcgcag
     1381 cacatagaac agcaggtaga aggtcagaat gccgttgatc agctgcgtca ccgaaccgcg
     1441 cagcagcagc gtgctgcggg aggtcagcca gcttgccagg tcggcgagca tgccggcggg
     1501 gttcagccgc tgctcgatcc agaccacgac ctcggcaatg cgcggatagc cggcggcggc
     1561 atcgcgccat tccgtatcgc gcagcaggtg ttccagatac ccggcgccga tcgccgcttc
     1621 gcggccaagt tggtgggcca cgaacagacc cggcacgatg atgaccacgc cggcaaccgc
     1681 cacgctgaca aacgccgcgg cgctcggcca gggcagcgag cgctcgatgc gccggtgcag
     1741 cggcagcagc aacaggccga gcaccaaccc catggtgagc ggagacagaa atggccgcgc
     1801 cagcgcgaag cagaagtaga atcccacggc cgcggcggcg aggaccacca gcgtgcggag
     1861 ctggctttgg ttcagctgcc attgctgcgt gctcattggt ctgcctcacg gcggacgagc
     1921 gggcgccatc cgttgccggg acgccgcggc aggtgcaggc cggctatccg cgtggtgtta
     1981 tgagaaaggt tgcatgcggg gacagcggtt gatggccgcg agtcccctcc ggaaactctg
     2041 gggtcggggg tcttcgcccg ccacgacctg atacgcgcga tccggggttg cacgttccgc
     2101 ggcttgcgct agcgcaccgt ccagggcgac cgctgctgcg ccatggcgga gagccgcgcg
     2161 atgcgctcct cgatcggcgg atgcgtggag aacaagctgc ccagatgcgc cccactcagc
     2221 gggttgacga tgaacaggtg tgcggtgg
//
LOCUS       DASSZH010000103         8610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_88000, whole genome shotgun sequence.
ACCESSION   DASSZH010000103 DASSZH010000000
VERSION     DASSZH010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8610
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88000"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VFN42_02040"
     CDS             complement(<1..782)
                     /locus_tag="VFN42_02040"
                     /inference="COORDINATES: protein motif:HMM:NF022457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo hydroxylase family protein"
                     /protein_id="HET8995427.1"
                     /translation="MPDGPSHFVMTPEQVLQQLPVEAWRGPFDASVRRRALEALESGK
                     VLVLPLPFAVLESEQRFLDAAIQSDRRKNLSFDPASGKLGNAALPAVEQAGLAAMMQR
                     FADSAFSLIMDLLPSYATHLARSRTSFRPAEIEGRTYSLRHDDRLLHVDAFPTRPLHG
                     QRILRLFTNVAPDGTQRKWHIGEPFADYAGRFLPEVKRPLPGSAWVLKMTGLTKGRRS
                     LYDHIMLGLHDAGKRDAAYQASCSLVAADFPPGTTWLCFTDQV"
     gene            complement(921..1583)
                     /locus_tag="VFN42_02045"
     CDS             complement(921..1583)
                     /locus_tag="VFN42_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019089025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HET8995428.1"
                     /translation="MFKGDLSTAGGRTLAWIDALFVDHAVFRLGWSNLAPVIPGKVYR
                     CNHPTPARLARLTQRLGLRTLINLRGRTGNGSDALSREAAARLGLDFIDMALESRGAP
                     QRDRILRLHDIYSTMRGPALIHCKSGADRAGLAAGLMLLFEGASAAQAMRQLSLRFGH
                     VRQAKTGILDAFFLRWQREGEGRKPFLDWVRDDYDEAVLRRDFRANGLASFLNDWILV
                     HE"
     gene            complement(1636..2268)
                     /locus_tag="VFN42_02050"
     CDS             complement(1636..2268)
                     /locus_tag="VFN42_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HET8995429.1"
                     /translation="MYLPPMFAEDRIEVLHAAIGRAGLAVLVTQSPDGLIASHVPLLL
                     DPEPAPYGTLLGHLARPNPQARGAIGNALAIFQGPDAYITPSWYATKRATGKVVPTWN
                     YVAIHACGPISFFDDADRLLDIVTRLTEREEGRRAEPWAVSDAPTDFVQGMLKGIVGF
                     ALPIAHLEGKWKMSQNRPAEDRAGVVDGLQADGRADVAAMVAEVARKPGA"
     gene            complement(2404..2775)
                     /locus_tag="VFN42_02055"
     CDS             complement(2404..2775)
                     /locus_tag="VFN42_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8995430.1"
                     /translation="MDRENASIVAVIDDDDEVRDVLRTLLETAGHDVETFKSGIHFLQ
                     HAELERMGCLVVDQRMPRMTGLELLLELERRGRPIPALLITGVHDPEIDAAASTIGAM
                     TVLEKPMSPQKLLRFVEFTLG"
     gene            3155..3928
                     /locus_tag="VFN42_02060"
     CDS             3155..3928
                     /locus_tag="VFN42_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995431.1"
                     /translation="MSENVKPLFDNETPTPATSSDPLSLDGSLMQAAEARMRRALGLE
                     GGAKLRAPERAESPQRQAERFTPGHNGHNGHKRRFVQDGEVPVTVVSSRRDAPGEVLP
                     HRGPPTGAGPNRLEAAETALNQEIATRQQAERALHEAQAVIHDLQTKLGHAELARIEA
                     VDGLHREQEAVAALRAELHACEERLRSAERSVRPRRERSVSFAPATLELTDDDADLSD
                     PHPIAAPKPESARRGRPPGKKAARSKLVRWWIKDEAKPD"
     gene            3999..4223
                     /locus_tag="VFN42_02065"
     CDS             3999..4223
                     /locus_tag="VFN42_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HET8995432.1"
                     /translation="MSNATPERVLAVTGMTCQGCVNAVTRVLSRVQGAQEVQVDLAAG
                     RAVVHGNAEPDALLAAVRKAGYGADLASAG"
     gene            4230..6740
                     /locus_tag="VFN42_02070"
     CDS             4230..6740
                     /locus_tag="VFN42_02070"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008850210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET8995433.1"
                     /translation="MDAMTPERVRLPVAGMTCATCAGRVEQALNALPGVQAVVNLADE
                     QAEIRFDPQQATLAALVEAVEDAGYDVPSEKLEFAIGGMTCATCAGRVEQALAQVAGV
                     DGAEVNLATEKAVVRGHAGLLRPAALVEAVQDAGYEATLLTGHAEREQALAEAEQVRL
                     RRLLWQVIAAAGLSLPLMLPMAGLMLPGWLQLLLATPVQFGIGARFYRAGWKALRAGS
                     GNMDLLVALGTSAAYFYSVYLIVSATSQHTYFEAAAVVITLVLFGRWLEARAKRSTGS
                     AIRALMALRPDTARVERHGAEIEVPVSAVALGDVVVVRPGERLPTDGRVTAGSSAVDE
                     SLLTGESLPVEKQPGDRVVGGSINGGGLLRVETTAVGEDSTLARVIALVEGAQTRKAP
                     VQRLVDRVAAVFVPIVLVCALVALVAWWVLAGSFSAGLIAAVSVLVIACPCSLGLATP
                     TALMVGTGAAARAGILIRDAEALERAHRIDTVVLDKTGTLTEGHPAVTEMLPVAVAEA
                     ELLRIAASAQAGSEHPLARAVLARAQAAASHLPGISQQVVDGGPSPVTMGNAGPGSDV
                     GAHGPSSAATGCTALGVLTDFQSHAGLGLSARVDGKRIAIGNRRLMDTHHVPLALDDR
                     ATELESQGRTVMWIASLDPQPALLGLIAVADPVKPHAAQAVRRLRGLGVEPILLTGDN
                     TRTAQAVARSLGINDVRAEVLPGDKAAHVERLQREGHQVAMVGDGVNDAPALAQADVG
                     IAMGTGADVAMQAAGITLMRGEPLLIADAIGISRATWRKIRQNLFWAFVYNVIGIPLA
                     GLGLLSPMLAGAAMAFSSVSVVSNALLLRRWRPAGGRR"
     gene            6737..7126
                     /gene="cueR"
                     /locus_tag="VFN42_02075"
     CDS             6737..7126
                     /gene="cueR"
                     /locus_tag="VFN42_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - intracellular copper ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HET8995434.1"
                     /translation="MNIGEAAQRSGVPAKTIRYYESIGLIGPAERSENHYRSYSDGDV
                     ATLRLVGRARRLGFSIEDLKNLVALYRDRGRASADVKALAQLHIGRLDRKLAELQTVR
                     AALAHLVERCHGDERPDCPILDELGAE"
     gene            7245..8417
                     /locus_tag="VFN42_02080"
     CDS             7245..8417
                     /locus_tag="VFN42_02080"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HET8995435.1"
                     /translation="MNRPLQHIRVLDFTTTIAGPYCARLLADLGATVIKIEAPEGDMM
                     RTRPPLRDGASTSFGQLNAGKQSLVLDLKQPAAQEIVRRLLAETDVLVENFRPGVMRR
                     FKLDYAALGDSYPALIYCAISGFGQTGPSAGLAAYAPAIHAASGHDLAHLNYQPGRER
                     PDNCGIYTADILTGTFAFGAINTALVHRQQTGRGQMIDVSMLESMLALLVSEVQTAQF
                     EVPPAGRPMFGPVRTKDGFIMPAVASERSFQGLARAAGHPEWITDPRFALYPDRRRHW
                     GTLIDELETWSTTLTTAECQAAFDREGVPSSPYRTVREVLADPQLSHRQALAEVRDAG
                     GSFRVVNPPFRLSGAVAEVRDFAAALGEHTTAVLREAGYSDGQIDAFAQAGVIRLE"
BASE COUNT         1398 a         2774 c         3044 g         1394 t
ORIGIN      
        1 acctggtcgg tgaagcacag ccaggttgtg ccgggcggaa agtccgccgc caccagggag
       61 caacttgcct gatacgccgc gtcgcgtttg ccggcgtcgt gcaggcccag catgatgtgg
      121 tcgtacaggc tgcgccggcc cttggtgagt ccggtcatct tcagcaccca ggcgctgccc
      181 ggcagtgggc gcttgacttc cggcaggaag cggccggcgt agtcggcgaa gggttcgcct
      241 atgtgccatt tccgctgcgt gccatccggc gccacgttgg tgaacagccg caggatgcgc
      301 tgcccatgca gcgggcgggt ggggaaggca tcgacatgca gcaaccggtc gtcatgccgc
      361 agcgagtagg tccggccctc gatctccgcc ggccggaagc tggttcgcga gcgcgccagg
      421 tgggtagcgt agctcggcag caggtccatg atcaggctga acgccgaatc ggcgaaacgc
      481 tgcatcatcg cggcgaggcc ggcctgttcg acggcaggca gcgcagcatt gcccagtttt
      541 ccgctcgccg gatcgaagct caggttcttt cgccggtcgg attgaatggc cgcgtcgagg
      601 aaacgctgtt cggattccag cacggcgaag ggcagcggca acaccagcac cttgccgctc
      661 tccagggctt ccagtgcacg ccgccgcacc gaagcatcga atggaccccg ccaggcctcg
      721 acgggaagct gctgcaacac ctgctccggc gtcatgacga aatggctggg tccatctggc
      781 atggttgaac caatgattga tcgcggcgag ggcgagggaa tcgaccgtcc cccaaagacc
      841 ggcgaagcat agccgagttg atatagggcg ccgctgacga aaccgtgagg tttgtggagc
      901 cgggaagttc ggattccggg ctactcgtgc accaggatcc agtcattgag aaagctcgcc
      961 agaccattgg cgcgaaaatc gcggcgcaat accgcttcgt cgtaatcgtc gcgcacccaa
     1021 tcgaggaaag gcttgcgtcc ttcaccctcc cgctgccagc gcaggaagaa ggcatcgaga
     1081 attcccgtct tggcttggcg cacatggccg aaacgcagcg aaagctggcg cattgcctgg
     1141 gcggcgctgg cgccctcgaa caacagcatc agccccgcgg ccaggccggc gcggtcggcg
     1201 ccggatttgc agtggatcag cgccggcccc cgcatggtgg aatagatatc gtgcagccgc
     1261 aggatgcggt cccgctgcgg tgcgccgcgg ctttccagtg ccatgtcgat gaaatccaga
     1321 cccagccgtg ctgccgcctc gcggctgagc gcgtcggagc cgttgccggt gcggccgcgc
     1381 agattgatca gcgtgcgcag tcccagccgc tgcgtcagcc gcgccaggcg cgcgggagtc
     1441 gggtggttgc agcgatacac cttgccgggg atgaccggtg ccagattgct ccagcccagg
     1501 cggaacacgg catgatcgac gaacagcgcg tcgatccagg caagcgtgcg gccgccggcg
     1561 gtgctcagat cgcccttgaa tatggtatcc atgcggaatg tgcgaacggg tcgggaacgg
     1621 gaagccggac tatgctcatg ctcccggctt gcgcgcaacc tcggcgacca tcgcggcgac
     1681 atcggcgcgt ccgtccgcct gcaggccgtc caccacgccg gcacggtcct cggccgggcg
     1741 gttctggctc atcttccact tgccctccag gtgcgcgatc ggcagggcga agccgacgat
     1801 gcccttcagc atgccttgca cgaagtcggt gggcgcgtcg gacacggccc agggttccgc
     1861 gcgccggccc tcttcgcgtt cggtcagccg ggtgacgatg tccagcaacc ggtcggcgtc
     1921 atcgaagaag ctgatcggac cgcaggcgtg gatcgcgaca tagttccagg tcggcacgac
     1981 cttgccggtg gcgcgcttgg tcgcatacca ggacggcgtg atgtaggcat ccggaccttg
     2041 gaagatcgcc agcgcattgc caatggcgcc gcgcgcctgc ggattcgggc gtgccaaatg
     2101 gcccagcagg gtgccgtagg gcgcgggctc gggatcgagc agcagcggca catggctggc
     2161 gatcaggccg tccggcgact gggtcaccag aaccgccagc ccggctcggc cgattgccgc
     2221 gtgcaagacc tcgatgcggt cttcggcgaa catcggtggc aggtacatgg cggtctcccg
     2281 tgcaagaggc ggttgggtcg cacggtgggc cggtggtgtc caattgccaa ttcgccgcca
     2341 ggggccggac cattacggcc cccactctgt ggcggtgtca gtccaatccc agaacagcgg
     2401 cggtcatccc agggtaaatt cgacaaagcg aagcagtttc tgtggcgaca tgggcttttc
     2461 cagcaccgtc atggcaccaa tcgtgctggc ggcggcgtcg atctcggggt cgtgcacgcc
     2521 ggtgatcagc aaggcgggaa tcggcctgcc acgccgctcc agttccaaca gcaactccaa
     2581 cccggtcatc cgcggcatgc gctggtccac caccaggcag cccatgcgct ccaattcggc
     2641 gtgttgcagg aaatgaatgc ctgacttgaa ggtttccacg tcgtgtccgg cggtttcgag
     2701 caaggtgcgc agcacgtcgc gcacttcgtc gtcgtcatcg atgactgcca cgatgctcgc
     2761 attttcgcga tccatatgcc tgtttccttc gttctggcgt cacgccacgc cggctggctg
     2821 gaaactagcc gggaccgggc agcggaagta tggccgaatc gagcggctca cttgctattg
     2881 tcgaagtcct gccttatgtg accggaccaa agccaatgct gagacgctgt ggtgccgggc
     2941 tgcaccgctg acgactgccc tggggcgggc agaccatctg gagatcatct tgcaggccag
     3001 aatttccgtc caacattgca ggacgctctg tgccttgtcc ggcatgaaca ggatgtcatg
     3061 gtatatattg cgcccgctat ggcatcgggt cgccgctggt tgcggcggtt acaaatgaaa
     3121 atgggtgcca accccaatct tccgggtcaa actcgtgtct gaaaacgtca agccgctatt
     3181 cgacaacgaa acgccgacac ccgccacttc ctccgatcct ctctcgctcg acggctcctt
     3241 gatgcaggca gccgaggcac gcatgcgccg tgcgcttggc ctggaaggtg gcgccaaact
     3301 gcgcgccccg gaacgggctg agtcaccgca acgccaggct gagcggttca ccccagggca
     3361 taacggtcac aacggtcaca agcggcggtt cgtccaggat ggcgaagtgc cggtgaccgt
     3421 ggtcagcagc cggcgtgacg ctccaggcga agtcttgccg catcgcggac cgccgaccgg
     3481 ggccgggccc aaccggctgg aagcggcgga aaccgcgctg aaccaggaaa tcgccacccg
     3541 ccagcaggcg gagcgtgcct tgcatgaggc gcaagccgtc atccatgacc tgcaaaccaa
     3601 gctcggccat gccgagttgg cgcggatcga ggcggtggat ggcttgcatc gcgagcagga
     3661 ggcggtggcc gcgctgcgcg ccgagctgca tgcctgcgag gagcgcttgc gcagtgccga
     3721 acgcagcgtg cgtcccaggc gggagcggag cgtttctttc gcgccggcaa cactggaact
     3781 gaccgacgac gatgcggacc tctccgaccc ccatccgatc gccgcgccga agcctgaatc
     3841 cgctcggcgg ggccggccac ccggcaagaa ggcggcgcgg tccaagctcg tacggtggtg
     3901 gatcaaggac gaagcaaaac cggactgacc ggtcttgacc ttccagtggt ggtaaccccc
     3961 atgtaagggg cgcaccagcc atcatgaagg aggttgcggt gtccaatgcc actccagaac
     4021 gcgtgctcgc ggtgaccggc atgacctgcc agggctgcgt caatgccgtg acccgcgtgc
     4081 tgtcgcgcgt gcaaggcgcg caggaagtcc aggtggatct cgcggcaggg cgcgccgtgg
     4141 tccacggcaa tgccgaaccg gatgcattgc tggccgcggt acgcaaggcc ggttacggag
     4201 cggacctcgc ctcggcgggg taggccgtga tggacgcgat gacgccggag cgggtccggc
     4261 tgccggttgc cggcatgacc tgcgccactt gcgccggacg cgtggagcag gcgctgaacg
     4321 cgctgccggg cgtgcaggcc gtggtgaatc tggccgacga gcaggccgag atccgcttcg
     4381 atcctcagca ggcgacgctg gcggcactgg tcgaggcggt agaagacgcc ggctacgacg
     4441 ttccctcgga aaagctggaa ttcgccatcg gcggcatgac ctgcgccacc tgtgcgggcc
     4501 gcgtcgaaca agcgctggcg caggtagctg gggtggatgg ggccgaggtg aaccttgcca
     4561 ccgagaaagc ggtggtgcgg ggccatgccg gcctgctgcg cccggccgct ctcgtggaag
     4621 cggtgcagga cgccggctat gaggcgaccc tgctgaccgg ccacgcggag cgtgagcagg
     4681 cgttggcgga agcggagcag gtccggctgc gccgcctgct ctggcaggtg atcgcggcgg
     4741 cggggctgag cctgccgctg atgctgccga tggcaggcct gatgctgccc ggctggctgc
     4801 aattgctgct ggcaaccccg gtgcagttcg gtattggcgc gcggttctac cgggccggct
     4861 ggaaggcgct gcgtgccggc agcggcaaca tggacctgct ggtcgcgctg ggtacatccg
     4921 cggcgtattt ctacagcgtt tacctgatcg tttcggcaac ctcgcagcac acctatttcg
     4981 aagccgccgc cgtcgtcatc accctggtgc tgttcggccg ctggctggaa gcgcgcgcca
     5041 agcgctccac cggctccgcc atccgcgccc tgatggcgct acggcccgac accgcgcggg
     5101 tggagcggca cggcgcagag atcgaggtcc cggtcagcgc ggtggcgctg ggcgacgtcg
     5161 tggtggtgcg ccccggcgag cgcctgccga ccgatggccg cgtcaccgcc ggcagtagcg
     5221 cggtggacga aagcctgctg accggtgaga gcctgccggt ggagaagcag ccgggcgacc
     5281 gcgtggtggg cggctctatc aacggcggcg gcctgctgcg ggtggagacc accgcggtcg
     5341 gcgaggactc cacgctggcg cgggtgatcg cgctggtgga aggtgcgcag acgcgcaagg
     5401 caccggtaca gcgtctggtg gaccgcgttg cggcggtgtt cgtcccgatc gtgctcgtct
     5461 gtgcgctggt ggcgctggtg gcgtggtggg ttctggccgg cagtttttcg gccgggttga
     5521 tcgccgcggt gtcggtgctg gtgatcgcct gcccgtgctc gctgggcctg gcgacgccga
     5581 ccgccctgat ggtcggcacc ggcgccgctg cgcgtgccgg catcctgatc cgcgacgcgg
     5641 aagcgctgga acgtgcgcac cgcatcgaca cggtggtgct ggacaagacc ggcacgctga
     5701 ccgagggcca tccggcggtg accgagatgc ttcctgtcgc cgtggcggaa gcggaattgc
     5761 tgcggattgc cgcctccgcc caggcgggca gcgaacaccc gctggcgcgc gccgtgctgg
     5821 cgcgggcgca agcggcggcg agccatcttc cgggcatttc ccaacaagtc gtggatggcg
     5881 ggccttcgcc cgtcacgatg gggaatgccg gtcccgggtc tgatgttggt gcccatgggc
     5941 cttcgtccgc cgcgacgggg tgtacggcgt taggggtgct gacggatttc cagagccatg
     6001 ccggactggg actcagcgca cgggtggacg gtaagcgcat tgccatcggc aatcgccgtc
     6061 tgatggacac gcatcacgtg ccgctggcgc tggacgatcg ggcgacggaa ctggaatccc
     6121 agggccggac cgtgatgtgg atcgcgtcgc tggacccgca gccggcgctg ctgggcctga
     6181 ttgcggtggc cgatccggtg aaaccacacg ccgcgcaggc ggtacgccgc ctgcgcggcc
     6241 tgggcgtgga gccgatcctg ctgaccggcg acaacacgcg caccgcgcag gccgtggcac
     6301 gcagcctcgg catcaacgac gtccgcgccg aggtgctgcc gggcgacaag gcggcgcatg
     6361 tcgagcgctt gcagcgtgag ggtcatcagg tcgccatggt gggcgacggc gtgaacgacg
     6421 caccggcgct ggcgcaggcg gatgtgggca ttgccatggg taccggcgcg gatgtggcaa
     6481 tgcaggccgc cggcatcacg ctgatgcgcg gcgagccgct gctgatcgcc gatgccatcg
     6541 gcatcagccg ggcgacttgg cggaagatcc ggcagaacct gttctgggcc ttcgtctata
     6601 atgtgatcgg cattccgttg gctgggctcg gcctgctcag cccgatgctg gccggtgcgg
     6661 cgatggcatt ctcttcggtc agcgtggtgt cgaatgcgct gctgctgcgg cgctggcggc
     6721 cggcgggggg caggcgatga acatcggaga ggcggcgcag cgttccggcg tgccggccaa
     6781 gacaatccgc tattatgaat ccatcggtct gatcggtccg gcggagcggt cggagaacca
     6841 ctatcgcagc tactccgacg gggacgtggc gacgctgcgg ctggtcgggc gggcgcggcg
     6901 gctggggttc tcgatcgagg atttgaagaa cctggtggcg ctgtatcgcg accgcggcag
     6961 agccagtgcc gatgtgaagg cgctggcgca gctgcacatc ggccggctcg accgcaagct
     7021 ggccgaactg cagacggtgc gcgctgcgct ggcgcatctg gtggagcgct gccacggcga
     7081 cgagcggccg gactgtccca tcctggacga actcggcgcg gagtagggga gtcggaacgg
     7141 cgcggccggc gggcatggcg ttcacgcgcc ggccggccgg acggcacagg tgtcagccgc
     7201 ggcagcgcct gctatgccgg ggagccgccc aacgaggaaa cgccatgaac cgacccctgc
     7261 agcacatccg tgtgctggac ttcaccacca ccatcgccgg cccgtattgc gcgcggctgc
     7321 tggcagacct cggcgccacg gtgatcaaga tcgaggcacc ggaaggcgac atgatgcgca
     7381 cgcggccgcc gctgcgcgat ggtgccagca ccagcttcgg ccagctcaat gccggcaagc
     7441 agagcctggt gctggacctg aagcagccgg ccgcgcagga gatcgtgcgc cgcctgctcg
     7501 ccgagaccga cgtgctggtg gagaatttcc gccccggcgt gatgcgccgc ttcaagctgg
     7561 actacgccgc gctgggcgac agctatcccg cgctgatcta ttgcgcgatt tccggattcg
     7621 gccagaccgg accctccgcc gggctggcgg cctatgcgcc ggccatccat gccgcatcgg
     7681 ggcatgacct ggcgcacctg aactaccagc ccggacgcga gcggccggac aattgcggca
     7741 tctacaccgc cgacatcctc accggcacct tcgccttcgg cgcgatcaac accgccctgg
     7801 tgcaccgcca gcagaccggc cgcggccaga tgatcgatgt ctcgatgctg gaatcgatgc
     7861 tcgcactgct ggtctccgaa gtgcagaccg cgcagttcga ggtgccgccg gccggccggc
     7921 cgatgttcgg gccggtgcgc accaaagacg gcttcatcat gccggcggtc gcaagcgagc
     7981 gcagcttcca ggggctggcg cgtgccgccg ggcatccgga gtggatcacc gatccacgct
     8041 tcgccctgta tcctgaccgc cgccgccatt ggggaacgct gatcgacgaa ctggaaacct
     8101 ggtccaccac gctgacgacc gcggaatgcc aggccgcgtt cgatcgcgaa ggcgtgccaa
     8161 gttcgcccta tcgtacggtg cgcgaggtgc tggccgatcc gcaactgtca catcgccagg
     8221 cgctggccga ggtgcgtgat gctggcggga gcttccgtgt ggtgaatccg cccttccgcc
     8281 tgtccggcgc ggttgccgaa gtgcgtgatt tcgccgcggc gcttggcgag cacaccacag
     8341 cggtgttgcg ggaggccggc tacagcgatg ggcagatcga cgcttttgcc caggcaggcg
     8401 tgatccggtt ggagtgaggc ggccgaggct cgggcggatt tccggaacgc agcgaaacag
     8461 atggcctacg cgaggagctc cttgtaggtc ggctccgtga caccgccctg gaagtactcc
     8521 gcctgcgccg gtccgccgtt cggatcgagg gagatcatgg cgaacccgtg gccgtataac
     8581 gggccagcca gaccgagttg ggtgccctct
//
LOCUS       DASSZH010000104         9186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1298317, whole genome shotgun sequence.
ACCESSION   DASSZH010000104 DASSZH010000000
VERSION     DASSZH010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 9186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9186
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298317"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="VFN42_02085"
     CDS             complement(<1..946)
                     /locus_tag="VFN42_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995436.1"
                     /translation="MPVSTQSFTDQAVQGLDFSGGTITETDTGAGVESFSGGTLVPQL
                     STFGLGPFHGISWGSFSAGVIAPGATLSVAIEYTVSDTNAGGLLAGLEQSFTGSGTAG
                     SALTIQESVYSDAAHSNLVGQITTNLSHKSSPSYSTGDVVLTNAYQTLYVDVQVTATV
                     DPLAAPGSQVTFSMLRQGFGQAGVTLDKQVSTDGTTWLEVGNGNFSNEPVVSAGSAVY
                     ERVLVVNSGSVAISNAQVTDAGGSGPTGFTFGGSAATGIAIGQTITSDIATLTAVTGE
                     QLDTATVTGTVAFGGRNVTVSANDQADYFATGTSGQGAA"
     gene            complement(2088..3611)
                     /locus_tag="VFN42_02090"
     CDS             complement(2088..3611)
                     /locus_tag="VFN42_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TSCPD domain-containing protein"
                     /protein_id="HET8995437.1"
                     /translation="MTQARTAYRRTPRQWHGVRMRLVSIGSDPDAAPRQVKMPAAWEE
                     SAAAALASLAPGSGAVSLTAAADAWIAPLAERAAASGHGRDLADQLHALLRLRRGAPD
                     PGVWQGTPEAVPGFVLNLPAFVDPSVGFAVPEFADAVDAAVIALTLAAPDARRIAVRL
                     ADLSGLLAALGVAYDSDAGRDVAGALAAVLRGRADLMSSRLAEGAAPSARAVWRLPPA
                     TTVVPGLAETATSLAQAARLAAAPRHLAATAVAAPTAADALLGVETGGIAPSFSPLSL
                     QGGLSRASRAWLAARGISPEAALAEMLAGENPLPAVTPAAYAAMHDAVAPFVHAMPPR
                     PEQRSAPDRPATRRELPGRRGGYTQKATVGGHKLYLRTGEYADGQLGEIFVALHKEGA
                     AFRGLMDNFAVAVSLGLQHGVPLDAFVQAFTFTRFGPAGAVEGDPAVHQATSLLDYVF
                     RHLASNYLGRQDLPEADAEPADSVGDGERDRSPLLPLELPSDADARVRRRAFRVIAS"
     gene            3786..4139
                     /locus_tag="VFN42_02095"
     CDS             3786..4139
                     /locus_tag="VFN42_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HET8995438.1"
                     /translation="MNIGTWLFTKLRGRQVGRDAAGNIYFEESRPRAGLRTRRWVAYA
                     GQPEASEVPPEWHAWLHYTTDAPLPESARRPWQKPHQPNTTGTAQSYRPPGHDYVGGQ
                     RARATGDYEAWTPGS"
     gene            4149..4679
                     /gene="mlaD"
                     /locus_tag="VFN42_02100"
     CDS             4149..4679
                     /gene="mlaD"
                     /locus_tag="VFN42_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HET8995439.1"
                     /translation="MPHRSLAEILTGALVLLVAVGFLGYAVAHSGRTGGSGYTLYADF
                     DNISGLSVGNDVRVAGVKIGTVTNETIDPKTFQARVSFTVQNDIKLPKDSAAEITSES
                     LLGGKYISLSPGGEQQMLQPGQTVTITQSSINIESLLGKFIFSASNLAGGGKQGQSGT
                     QGGSGSGELAPLGGGK"
     gene            4679..4894
                     /locus_tag="VFN42_02105"
     CDS             4679..4894
                     /locus_tag="VFN42_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995440.1"
                     /translation="MAPAPEAWPGADGSPISCREKLRMLAENHAELAQSLQDAFEDAV
                     LMGVDEQAMRHILVAMVEGLESPKRGR"
     gene            5353..5625
                     /locus_tag="VFN42_02110"
     CDS             5353..5625
                     /locus_tag="VFN42_02110"
                     /inference="COORDINATES: protein motif:HMM:NF021444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
                     /protein_id="HET8995441.1"
                     /translation="MIQVLDKPDAESRILSIKAGQSATYRSLTITVKACAIRPPDLPP
                     DSTAFLDITDSHPGQPSFSGWMLASDPSLNMMQNPIYNVHVMGCTP"
     gene            5622..6401
                     /gene="otsB"
                     /locus_tag="VFN42_02115"
     CDS             5622..6401
                     /gene="otsB"
                     /locus_tag="VFN42_02115"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HET8995442.1"
                     /translation="MTSGIPDQTLPDFARTALLLDVDGTLIDIAPRPDLVVVPPGLVQ
                     TLHTLRGALDGALAVISGRPVEQVDLLLDQAPHAVAGEHGGAVRHAPDQPIERPPLPT
                     PPAEWLAAAERLAQAHRGVLLEHKARGFVVHYRAVPELGPVLQDAVLEILGGSREFAV
                     LAAHMAWEVRPRGTDKGIAVAALMQRAPMHGRIPLFIGDDVTDEDAIVKAREMGGIGL
                     RVDAAFGSAGDVRAWLADAARRGAWPALPLPGSAQADHPDG"
     gene            6429..7505
                     /locus_tag="VFN42_02120"
     CDS             6429..7505
                     /locus_tag="VFN42_02120"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HET8995443.1"
                     /translation="MTQSLADTPAAWTAADLRDNEPRWVFPLDDMARRDVLRAVNRLD
                     PDADLLTLSRDAVDLGAAAPILAAALREVRDGRGIALLRGLPRQELDEAAFRRLTWAL
                     GLHAGVARPQNRASDYLARVRDAGSVYRSAGGRGYSSNAELDFHTDSADVVMLACFNP
                     AREGGQSMVTSSVAAHAVLVDEAPDLAEALHQTFWFSRQTEQAADESPAYPNPIFDSA
                     DGLLCSKWNRNRIRTAQQIEGVPPLSGMQRAAMDALDEVLRRPALMHAMYLRPGDVQI
                     LNNHVTLHSRTEFVDHEEPARKRLLFRLWLATPDSPRLPESWRPAYRSVAPGTVRGGI
                     RGQAYDAHRRAFESRQAAALGMTS"
     gene            complement(7528..8493)
                     /locus_tag="VFN42_02125"
     CDS             complement(7528..8493)
                     /locus_tag="VFN42_02125"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HET8995444.1"
                     /translation="MSTPVLEVRNLSKRFPVVRGFLRRTVAELRAVDDVSLHIEPGET
                     LCVVGESGCGKSTVGKLVLRLLEPSDGRILIEGRDVTGLSAERVRPFRRRVQMVFQDP
                     YASLNPRLSAAEIVTEPLENFQRLSGSERRDRAADLLARVGLRPDMVDRRPFEFSGGQ
                     RQRLGIARALSVQPALIVADEPVSALDVSVQAQVLNLMLDLQESDGLAYLFISHDLGV
                     VEHIGHRIAVMYLGRIVELADRDALFARPQHPYTETLLQAAPVPDPRARRERPIIEGE
                     VPSPLNPPSGCAFHPRCPYAVDRCKSEVPALRRLEDGRLAACHLR"
     gene            complement(8490..>9186)
                     /locus_tag="VFN42_02130"
     CDS             complement(8490..>9186)
                     /locus_tag="VFN42_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8995445.1"
                     /translation="AMIFQEPMTSLNPVHTIGEQIAEVVRIHQGASRSAALAHAQEML
                     RTVRIPDAPRRVHDYPHHFSGGMRQRAMIAMALACSPSLLIADEPTTALDVTIQAQIL
                     RLMLELKERIGAAILMITHDLGVVAETCQRVIVMYAGQIVEEATVQELFDHPSHPYTR
                     GLMGSIPRGADRRRKRLPEIPGVVPNLAEPIAGCAFAPRCGLAIDACRAAPVALRSVA
                     PGHRVACIRAEVA"
BASE COUNT         1671 a         3148 c         2938 g         1429 t
ORIGIN      
        1 gagccgcgcc ctgaccgctg gtccccgttg cgaagtagtc cgcctggtcg ttggccgaaa
       61 cggttacgtt cctgccgccg aaagcgacgg tgccggtcac tgtcgcggta tcgagctgct
      121 cgccggtgac ggccgtcagg gtggcgatat ccgaggtgat ggtctggccg atggcgattc
      181 cggtggccgc gctgccaccg aaggtaaaac cggtcgggcc gctgccgccc gcatcggtta
      241 cctgcgcatt ggagatcgcc accgagccgg aattgacgac cagaacgcgc tcataaaccg
      301 cggaacccgc cgacacgacc ggttcgttgg agaaatttcc attgccgact tccagccagg
      361 tggtaccgtc ggtggaaacc tgcttgtcca gcgtgacccc ggcttggccg aagccctgcc
      421 ggagcatgct gaatgtcacc tgtgaaccag gtgccgctaa tggatcgact gtggcagtta
      481 cctgtacgtc aacatacaac gtctgataag cattggtcag gacaacatcg ccagtggagt
      541 agcttggcga agacttgtgt gataaatttg tcgttatctg accaaccaag ttgctatgcg
      601 ctgcatcaga atacacactc tcctggatgg tcagagcgct acctgcagtt cctgaaccag
      661 taaagctttg ctccagccca gcgagcagtc cacctgcgtt cgtatcacta acggtatatt
      721 caatagccac cgacagggta gcacccggag caatcacacc ggcagaaaag cttccccagc
      781 taatcccgtg aaacgggccg agcccgaacg tactcagctg cggaaccagc gtgccgccgc
      841 taaaactttc gactcctgcg ccagtatcag tttcggtgat tgtgccacca ctaaagtcga
      901 ggccttggac cgcctggtcc gtgaagctct gagtcgacac cggcatgcct ccggcagcca
      961 ttcaggctgc gtacaccctt cgtgtggatt tgagatagtt ccgcagtcgc caagaaggct
     1021 aagatcgacg agaccgccgg atcggaagca gaagcgccgc caacccgaac gctagcacac
     1081 tgaggccgct cggttccgga acagtaaagg tctgggtgac cgagttgatg gtcgcggttc
     1141 caccctgact cgagtctgcc cgaatatcgg caacgatctg aacgctggta aaaggtccgg
     1201 gtacggtcgg atcacctgac gaataggtcg ttgcgatcgt tggaccacct gcgacggctg
     1261 tcggcgctcc cagtccatag cccgaggtgt tgtcggagta ggtagcggac acgtccgccc
     1321 cgacggctcc cccggtgaca cctccgctga cttgcaaaag ctctccgctt aggatcgcgg
     1381 tggttgtaga gacgttgaag gtgacgctga cgtccgccaa gctgccgctg gagaacagca
     1441 atggcccggc gcctgtggga ctgatgacaa atccatcatt aatcgctagc gctgtcattt
     1501 tcatggagct acactgcgtt acttcacaat cgtagactga cacagtataa ggcccgaagt
     1561 tgatagtcgt atttgagtcg ccccacgtaa cactttgggc gccggcctgg ccggtccagg
     1621 acaccagacc agccgccgcg agacatccaa cccagatcgc gctctttagc cccatcgcgg
     1681 ccatcctacc ccggttccgg cgaacgatca gtcatccgtt aatggatcgt taaacggacg
     1741 atcatcgatt gtaaagcgaa aagatgtctc attttccgta tacatccatg gaaagaggct
     1801 gttatgctag aaatcgtata attttccggg aatggtatca tccatagcga cataggggcg
     1861 tcgctgcgcg gatcgcaata gcaaaaaggc gaccaattca ccaaagtacg ccattccgac
     1921 ccggtgctgg attccagtca cagcgtccga acagcgctgg aagcgccgcg gcacaatcac
     1981 cgcgagagac ctgtcccgcc gccgaaaaat tgtcgcgcgc agttacccga cgctggttcc
     2041 ctgcgcgccg gtcaaggccg gcgccacagc accggggacc tacaacttta gcttgcaatc
     2101 acccggaatg cccgccggcg cacccgcgca tcggcgtcgg acggcagctc cagcggcagc
     2161 agcggcgatc gatcccgctc accatcgccc accgaatccg ccggttcggc atccgcctcc
     2221 ggcagatcct gccggcccaa atagttgctg gccaagtgac ggaacacgta atccagtaac
     2281 gacgttgcct ggtgcaccgc tggatcgcct tctactgcac cagccggccc gaaccgcgtg
     2341 aaggtgaacg cctgcacaaa tgcgtccagc ggcacgccgt gctgcagccc caggctgacc
     2401 gccacggcaa aattgtccat cagtccgcgg aacgcggcac cttccttgtg cagcgcgaca
     2461 aagatctcgc cgagctggcc gtcggcgtac tccccggtgc ggagatagag tttgtgccca
     2521 cccaccgtgg ccttttgcgt gtacccgccg cgccgtcccg gcagttcgcg tcgagtcgca
     2581 gggcgatccg gcgcgctgcg ctgctccggg cgcggcggca tggcatgcac gaagggggca
     2641 accgcatcgt gcatcgccgc gtacgccgcc ggcgtgacgg ccggcagcgg attctcgccc
     2701 gccagcatct ccgccagcgc cgcttccggc gaaatgcccc gagccgcgag ccaggcgcgg
     2761 ctggctctgc ttaatcctcc ctgtagcgac aacggtgaga agctcggcgc gatccccccg
     2821 gtctccaccc caagcagggc atcggcggcc gtcggagcgg caaccgcggt cgcggcgagg
     2881 tgacgcggag cagcagccag ccgagccgcc tgggcaaggg aagtcgccgt ctcggcaagg
     2941 ccaggcacga ccgtcgtggc cgggggaagc cgccagacgg cacgggccga aggagccgcc
     3001 ccttctgcca ggcgggagga catcagatcg gcgcgccccc ggagaacggc ggccagcgcg
     3061 ccggccacat ccctgcccgc gtcagaatca taggcaacgc cgagcgccgc cagcaggccc
     3121 gataaatcag ccaggcgcac cgcgatccgg cgcgcgtcgg gcgccgccag ggtgagggcg
     3181 attacggcgg catccaccgc atcggcgaat tccggcacgg cgaacccgac ggagggatcg
     3241 acaaaagccg gcaggttcag cacgaaaccc ggaaccgcct ccggcgtccc ctgccaaacg
     3301 cccgggtctg gcgcgccacg gcgcagccgc agcaaggcat gcagttggtc cgcaaggtcg
     3361 cggccgtgcc cgctggctgc cgcacgctcg gccagaggcg cgatccaggc atccgccgcg
     3421 gcagtcaatg acaccgcacc gctgcccggc gccagggagg ccagcgccgc cgcggcactc
     3481 tcctcccagg cggcgggcat tttcacctgg cggggcgccg cgtccgggtc gctgccgatc
     3541 gatacaagcc gcatccgcac gccgtgccac tgccgcggcg tgcggcgata cgctgtccga
     3601 gcctgggtca tgccggcatc ctatgcccgc cgcaacgact ccggaagggt attttgtggt
     3661 ccgacaagca ggtcaaccac aaaatcctgt ggattacccg agatcgccta agggccgaaa
     3721 cggaactgcc aaaagtcgaa ccggaacggt ggaccaaccg cgcgcaggct cgtatatgcc
     3781 gcggcatgaa catcggaacc tggctgttca ccaaactgcg aggccggcag gtcggccgcg
     3841 atgcggccgg caatatctat tttgaggaga gccgtccccg cgccggtctc cgtacccgcc
     3901 ggtgggtggc ctatgccgga cagccggagg ccagtgaagt gccgccggag tggcatgcct
     3961 ggttgcacta caccacggat gcgccgctgc cggagagtgc ccggcgcccc tggcagaagc
     4021 cgcaccagcc gaacaccacc ggcacggcgc aatcctaccg tccgcccgga cacgattatg
     4081 tgggcggcca gagagcgcgc gccactggcg actacgaagc ctggacaccc ggcagctagg
     4141 ccggacaaat gcctcatcgc agcctcgccg agatactcac cggcgctctg gtgttgctgg
     4201 tcgcggtcgg cttcctcggc tatgcggtcg cccattccgg ccgaaccggc ggcagcggct
     4261 acacgctcta cgcggatttc gacaacatca gcggattgtc ggtcggcaat gatgtccgcg
     4321 tggcaggcgt caagatcggc acggtgacca acgagactat cgatccgaag accttccagg
     4381 cccgggtctc cttcaccgta cagaacgaca tcaagctgcc gaaggacagc gcggccgaga
     4441 tcaccagcga aagcctgctc ggcggtaaat acatctcgct ttcccccggc ggcgaacagc
     4501 agatgttgca accaggccag accgtcacca tcacccaatc ctcgatcaac atcgagagtc
     4561 tcttgggcaa gttcatcttc agcgcgagca atctggccgg cggcggcaag caggggcagt
     4621 cgggaacgca aggcggcagc gggtccggcg aactggcgcc cctgggcggc ggcaagtgat
     4681 cgccccggct cccgaggctt ggcccggcgc cgatggcagc ccaatctcgt gccgcgagaa
     4741 gctgcgcatg ctggcggaga accatgccga actggcgcaa tcgctgcagg atgcgttcga
     4801 ggacgcggtg ctgatgggcg tcgatgagca ggcgatgcgc cacattctcg tcgccatggt
     4861 cgaagggctg gagtcgccca agcgcggccg atgaccttgc gccgcttcgc cttgcagcag
     4921 cttcgtcctg atcgggggct cgggtggcgg ttgcgctgca ttatcgcagt gggcgcaacc
     4981 atccccgccc tggcgctggc acaggcaccg ccggcaccgg ccggcaccac acccttgctg
     5041 ccgccaaccc ggcaatatcc gccgtcggat attcaaagcg agccgctggc gccgccgcct
     5101 ggagctgcct cttccgcgtt gccagcgcag acccagagcg tccccacgcc ggcctcaaca
     5161 cagccgccgg acgggatcgc gcctgctttg ccgccggcca ccgggacggt ccccgagtcc
     5221 gctcccccgc cgacggcgcc cggccagccg gagcccagcg cgccgcccgg cgcactgcag
     5281 agcagcccgg gccaaccacc tgctgccgcg gcgccccccc cgccgaaccc gtgggttccg
     5341 cagggcggcg cggtgatcca ggtgctggac aagccggatg cggaaagcag gatactgtcc
     5401 atcaaggccg gccagtccgc cacctaccga tccctgacga tcacggtgaa agcctgcgcg
     5461 atccgtccac ccgacctgcc gccggattcc accgcgttcc tcgacatcac cgacagccat
     5521 cccggccagc caagcttcag tggctggatg ctggcatccg atccatcgct gaacatgatg
     5581 cagaacccga tctacaatgt gcatgtgatg ggttgcacgc cgtgacgtcg gggattccag
     5641 accagactct gccggacttc gcccgcaccg cattgctgct ggatgtcgac ggcacgctga
     5701 tcgacattgc accgcgcccc gaccttgtgg tggtaccgcc cggattggtg cagaccctgc
     5761 acacattgcg cggcgccctc gacggagccc tggccgtgat cagcggccgc cccgtcgagc
     5821 aggtggatct gctgctggac caggcgccgc acgcggtggc cggcgagcac ggcggcgcgg
     5881 tgcgccacgc gccggatcag cccattgaac gcccacccct gcccaccccg cccgccgaat
     5941 ggctggccgc ggcggaacga ctggcgcagg cgcatcgggg cgtgctgctg gagcacaagg
     6001 cgcgcggctt cgtcgtgcat tatcgcgccg tgccggaact ggggccggtg ctgcaggacg
     6061 cggtgttgga gatcctgggc ggcagccggg agttcgcggt gcttgccgcg catatggcgt
     6121 gggaggtgcg gccgcgcggc accgacaagg ggatcgcggt tgccgcgctg atgcagcgcg
     6181 cacccatgca cggccggata ccgctgttca ttggcgacga cgtcaccgac gaggatgcca
     6241 tcgtcaaggc gcgcgagatg gggggcatcg ggctgcgggt cgatgccgcc ttcggttcgg
     6301 ccggcgacgt gcgcgcctgg ctggccgacg cggcacggcg gggcgcctgg ccggccctgc
     6361 cattgccagg ttcggcgcaa gcggatcatc ctgacggctg atccctcccg ctgcgagcgg
     6421 agaagccgat gacgcagagc ctcgctgaca cgccggcggc atggaccgcc gcggacctgc
     6481 gcgataatga gccgcgctgg gtgttcccgc tcgacgacat ggcacggcgg gacgtgctgc
     6541 gcgccgtcaa tcgcctggac ccggacgcgg atctgttgac gctgagccgg gacgccgtcg
     6601 acctgggtgc cgctgctccg atcctggctg ccgcgctgcg ggaagtgcgc gacggccgcg
     6661 gcattgccct gctgcgcgga ctgccgcggc aagagctgga tgaggctgca ttcaggcgcc
     6721 tcacctgggc gctgggtctg cacgcgggcg tcgctcggcc acagaaccgc gccagcgact
     6781 atctggcgcg ggtacgcgac gcaggcagcg tgtaccgcag cgccggcgga cggggctact
     6841 catccaatgc cgaactggat ttccacaccg atagcgccga cgtggtgatg ctggcctgct
     6901 tcaatccggc gcgcgaaggc gggcagagca tggtgacgag ttcggtcgcc gcccacgcgg
     6961 ttctggtgga cgaggcgccg gacttggcgg aggcgctgca ccagaccttc tggttcagcc
     7021 ggcaaaccga gcaggcagcc gatgaatcgc cggcctaccc gaatcccatc ttcgacagcg
     7081 ccgatgggct gctatgcagc aagtggaacc gcaatcgcat ccggacggcc cagcagatcg
     7141 aaggcgtccc cccgctgagc ggaatgcagc gtgcagcgat ggatgccctg gacgaggtgc
     7201 tgcgccggcc ggcgttgatg cacgcgatgt atctgcggcc aggcgacgtg cagatcctga
     7261 acaatcacgt gaccctgcat tcgcgcaccg aattcgtcga ccacgaggaa ccggcccgca
     7321 agcgcctgct gttccgcttg tggcttgcca cgccggattc tccgcgcttg ccggaaagct
     7381 ggaggcccgc ctatcggtcg gttgccccag gcacagtgcg tggcgggatc aggggacagg
     7441 cgtacgacgc acatcgccgc gcgttcgaat cccgccaggc cgccgcgctt ggcatgacga
     7501 gctgagccgc tccgctgggg ccgttaccta gcgcaaatga cacgccgcga gccggccatc
     7561 ctccagccgc cgtagcgccg gcacttcgct cttgcagcga tccaccgcgt acggacaacg
     7621 cggatgaaac gcacaccccg acggcggatt cagcggactc ggtacctcgc cttcgatgat
     7681 cggacgttcc ctcctggcgc gcggatccgg taccggcgct gcctgcagca gcgtctcggt
     7741 gtacgggtgc tgcggccggg caaacagcgc atcgcgatcg gcaagctcta cgatgcgccc
     7801 gagatacatc accgcgatgc ggtgcccgat atgctccacc acgccgagat catgcgagat
     7861 gaacaggtac gccagcccgt cgctctcctg cagatccagc atcaggttca atacctgcgc
     7921 ctggacggag acatccaagg ccgacaccgg ctcgtccgcg acgatcagtg ccggctgaac
     7981 gctcagcgca cgggctatgc ccagccgctg ccgctgccca ccggaaaatt cgaatggccg
     8041 ccgatccacc atgtccggcc gaaggccgac gcgcgccagc aggtcggccg cgcgatcgcg
     8101 gcgttccgac ccggacagac gctggaaatt ctccaatggc tcggtgacga tctccgccgc
     8161 ggacaggcgc gggttcagcg atgcgtatgg atcctgaaac accatctgca cccgccggcg
     8221 gaacgggcgc acacgctccg ccgacaagcc ggtcacgtcg cggccttcga tcaggatgcg
     8281 cccatccgaa ggctccagca ggcgcagcac cagcttgccg accgtggact tgccgcagcc
     8341 ggactcgccg acaacgcaca gtgtttcacc cggctcgata tgcagcgaga catcgtccac
     8401 cgcccgcaat tccgcgacgg tacggcgcag gaaaccgcgc accaccggaa agcgcttgga
     8461 caggtttcgc acttccagga cgggcgtgct catgcaacct ccgcacggat gcaggcgacg
     8521 cgatgacccg gcgcgaccga gcgcagggcg acgggcgctg cccgacatgc gtcgatcgcc
     8581 aacccgcagc gcggtgcgaa ggcgcagccg gcgatcggct ccgccagatt gggcaccacg
     8641 ccggggatct ccggcaggcg cttccggcgc cgatcggcgc cgcgcggaat cgatcccatc
     8701 aggccgcgcg tgtagggatg cgacggatga tcgaacagtt cctgcaccgt cgcttcttcc
     8761 acgatctgcc cggcgtacat cacgatgacc cgctggcagg tctccgccac cacgcccagg
     8821 tcgtgggtga tcatcaggat tgctgcgccg atgcgttctt tcagttccag catcagccgc
     8881 aggatctgcg cctggatcgt cacgtccagg gcggtggtgg gctcatcggc aatcagcagg
     8941 cttggcgagc aggccagcgc cattgcgatc atcgcccgct ggcgcatgcc gccggagaaa
     9001 tggtgggggt agtcatgcac ccggcgcggc gcatcaggga tgcgcacggt gcgcagcatc
     9061 tcctgcgcat gcgccagcgc ggctgagcgg gaggcgccct gatggatgcg caccacctca
     9121 gcgatctgtt cgccaatcgt gtgcaccgga ttcaggctgg tcatcggttc ctgaaagatc
     9181 atcgcg
//
LOCUS       DASSZH010000105         3508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_377883, whole genome shotgun sequence.
ACCESSION   DASSZH010000105 DASSZH010000000
VERSION     DASSZH010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377883"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VFN42_02135"
     CDS             <1..919
                     /locus_tag="VFN42_02135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8995446.1"
                     /translation="AGHDTGARDTGARDTGARDTGARDTGARDTGARDTGARDPGGHD
                     AQTLLCFYDIDTPVTLRKLAEGDTEYLSAAHIPVFDLYFSFTGGPTLRLLEAHYGARA
                     ARALYCSVDPEKYHPTGVPRRWALGYLGTYSTDRQPALERLLLQPARRLPHLRFVVAG
                     SMYPADIAWPDNVDRIEHLPPDQHADFYASLDWALNVTRADMLRAGYSPSVRLFEATA
                     CGTPVISDPWPGLDTLFEPGREILTASDSSAVERLLTTPEPLRQEIARAGRRRTLEQH
                     TAAHRAEELETHLRSAAAQAAPPEQGAVR"
     gene            916..1950
                     /locus_tag="VFN42_02140"
     CDS             916..1950
                     /locus_tag="VFN42_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012569142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HET8995447.1"
                     /translation="MTRGADRTVLIAGGAGFIGSHLCEAMLADGARVICLDNLLTGRP
                     QNIRHLRSEPRFEFIQADVIDRLPKLPRLDAVLNLACAASPPRYQADPVHTLMTSVVG
                     TRNLLSLAQDNRARFLLASTSEVYGDPEVHPQKESYLGNVNPTGPRACYDEGKRAAET
                     LCFDHLRLGRVDVRVARIFNTYGPRMSPDDGRVVSNVVNQALWGAPITLYGDGRQTRS
                     FCYVDDLVGGLQSLLMHPGRLSGPVNLGNPAEITVAALVDMVIRMSRSRSPVVNRPLP
                     VDDPRRRCPDITQARELLGWEPAIPLEQGLRRTITWFAAEQGIRPHLVQVAPAPHGGR
                     GARVRAAAGD"
     gene            complement(1960..2214)
                     /locus_tag="VFN42_02145"
     CDS             complement(1960..2214)
                     /locus_tag="VFN42_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995448.1"
                     /translation="MSDATPDGRGALHASLREVEPGLFRAEYVGELNPANPDARAFPD
                     THLGTSEQDVKIWVEQMARSLGYQSVIWDPLPGAPSRAAG"
     gene            2340..2507
                     /locus_tag="VFN42_02150"
                     /pseudo
     CDS             2340..2507
                     /locus_tag="VFN42_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004143718.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
     gene            2534..2753
                     /locus_tag="VFN42_02155"
                     /pseudo
     CDS             2534..2753
                     /locus_tag="VFN42_02155"
                     /inference="COORDINATES: protein motif:HMM:NF022150.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="KGG domain-containing protein"
     gene            2857..3375
                     /locus_tag="VFN42_02160"
     CDS             2857..3375
                     /locus_tag="VFN42_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008851031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET8995449.1"
                     /translation="MNAMTDRAREVYLTGLRNQHAVEHQAIELLERQIGRLEHYPEME
                     ARMRQHLEESREQARRIEDLLSGLGSSHSSLKDTAMSLVGNLMAIGHTPAGDEVIKNT
                     MANFAFEHYEIAGYKALLVLADTVGHAPAKAALTTSLKEEEAMALWIDSHIADTTRRF
                     VERSAAGKSAKV"
BASE COUNT          604 a         1221 c         1180 g          503 t
ORIGIN      
        1 cgccggccat gacacagggg cgcgtgacac aggggcgcgt gacacagggg cgcgtgacac
       61 aggggcgcgt gacacagggg cgcgtgacac aggggcgcgt gacacagggg cgcgtgaccc
      121 cggcgggcat gacgcacaga ccctgctctg cttctacgac atcgacactc cggtcaccct
      181 gcgcaaactt gccgagggcg acacggaata cctgtcagcc gcacacatcc cggtgttcga
      241 cctgtacttc tccttcaccg gcggccctac cctgcgcctg ctggaagcgc actacggcgc
      301 ccgcgccgcc cgcgcgctgt attgctcggt cgatccggag aaataccacc ccaccggcgt
      361 accgcggcgc tgggcgcttg gctacctcgg cacctacagc acggaccgtc agcccgcgct
      421 ggaacgcctg ctgctgcagc cggcgcgccg cctgccgcat ctgcgcttcg tggtcgccgg
      481 atcgatgtat cccgccgata tcgcctggcc ggacaatgtg gatcgcatcg agcatctgcc
      541 gcccgaccag catgcggact tctacgccag cctggactgg gcgctgaacg tcacgcgcgc
      601 cgacatgctg cgcgccggct acagccccag cgtgcggctg tttgaagcca ccgcctgcgg
      661 cacgcccgtg atctccgacc cctggcccgg gctcgacacg ctgttcgaac cgggacgcga
      721 gatcctcact gcctccgaca gcagcgctgt ggaacgactg cttaccacgc ccgaaccgct
      781 gcggcaggag atcgcccgcg ccgggcgccg ccgcacgctg gagcagcaca ccgccgcgca
      841 ccgggccgag gaacttgaaa cgcatctgcg gagtgccgcc gcgcaggcag cgccccccga
      901 acaaggagcc gtacgatgac acgcggtgcg gacagaacag tgctgatcgc aggcggcgcc
      961 ggcttcatcg gctcgcatct ctgcgaggcg atgctggccg acggcgcgcg ggtcatctgc
     1021 ctcgacaatc tgctgaccgg ccggccgcag aatatccgcc acctgcgcag cgagccccgc
     1081 ttcgagttca tccaggccga cgtcattgac cgcctgccga agctgccgcg cctggacgca
     1141 gtgctgaacc tggcctgtgc cgcctcgccg ccgcgctacc aggccgatcc ggtgcacacg
     1201 ctgatgacca gcgtcgtcgg cacgcgcaac ctgctgagcc tggcgcagga caatcgcgcg
     1261 cgctttctgc tggcctccac cagtgaggtg tatggcgatc cggaagtaca cccgcagaag
     1321 gagagctacc tcggcaacgt caatccgacc ggtccgcgtg cctgctacga cgaaggcaag
     1381 cgtgccgcgg agacgctgtg cttcgaccat ctgcggcttg ggcgcgtgga cgtgcgggtc
     1441 gcccgcatct tcaacaccta cggcccgcgc atgagcccgg atgacgggcg cgtggtgtcg
     1501 aatgtggtga accaggcgct gtggggcgcg ccgatcacgc tgtacgggga cggccggcag
     1561 acgcggtcgt tctgctatgt cgatgacctg gtcggcgggt tgcaaagcct gctgatgcac
     1621 ccggggcggc tgtccggccc ggtgaaccta ggcaacccgg cggagatcac cgtggccgcc
     1681 ctggtggaca tggtgatccg catgagccgc tcccgctcgc cggtggtgaa ccgaccgctg
     1741 ccggtggacg atccgcgccg ccgctgcccc gacatcaccc aggcccgcga actgctcggc
     1801 tgggagccgg ccatcccgct ggaacagggc ttgcgccgca ccatcacctg gtttgccgcg
     1861 gaacagggga tccggccgca tctggtgcag gtcgcgccgg cgccgcacgg cgggcgcggc
     1921 gcccgcgtgc gtgcggcggc gggcgactga gccggccggt cagccggcgg cgcgactggg
     1981 agcgccgggc agcgggtccc agatcacgct ttgatagccg agtgagcggg ccatctgttc
     2041 aacccaaatt ttcacgtcct gctcgctggt gccaagatgg gtatcaggga aggcacgcgc
     2101 gtcgggattg gccggattca gttcaccgac atattccgcg cgaaacaagc ccggctcgac
     2161 ttcgcgcagg ctggcatgaa gcgcaccacg accgtccggg gtcgcatctg acataacgag
     2221 gttcctccac ctgtccttgc cataacgcga cgtcacccgg ctgggttgaa ccgggctgtc
     2281 acgcaacttg ggccgatgcc gccccgttgg ggccaggtgg gatggaagga acctggccca
     2341 tgccagaaca gcatacccgc aatcgcggga attttgccga ggatcgcgac aaggcgtcgc
     2401 gcgcaggacg ggttggcggg cagcgcagct ccgggaactt cgccaatgac cgcgcgcgcg
     2461 cggtggaggc gggccgcaag ggtggcgagc gcagccatgg cggcggcggt gcacggcaca
     2521 atccaggcaa tttcgcccag gatcgcgaaa aggcggtgga agccgggcgg cgcggcggcc
     2581 agcagagcca tgcgcatgag tcgcccgaac cggtcgagca gcgcatccac cgggcggaac
     2641 tgcccggcca gcgcgattca ctgccgcccg gatcgctcgg cggcctgccg ccggagccgc
     2701 aaggcgatga gaatcgcgat cctccgccgc aggcgccggt catcccggaa tagccctccg
     2761 ctgcgcttgc catttgccgg tgagccaagg cctgcacagc aacttcggcc cgtgcggcgc
     2821 attcatcaga cctcacgaaa aacgcggaga ggaccgatga atgcgatgac cgaccgggcc
     2881 cgcgaggttt acctcacggg cctgcgcaac cagcacgccg tcgagcacca ggcgatcgaa
     2941 ctgctcgagc gccagatcgg ccggctggag cactacccag aaatggaggc gcgcatgcgc
     3001 cagcacctgg aggaaagccg cgagcaggcg cgccgcatcg aagacctgct gtccggtctt
     3061 ggcagcagcc attccagcct gaaggacacg gcgatgtcgc tggtcggcaa cctgatggcg
     3121 atcggccaca cgccggcggg cgacgaggtg atcaagaaca ccatggcgaa cttcgccttc
     3181 gagcactacg agatcgccgg ctacaaggcg ctgctggtgc tggcggacac ggtcggccac
     3241 gcgccggcga aggccgcgct gaccacctcg ttgaaggagg aggaggcgat ggcgctatgg
     3301 atcgacagtc acatcgccga caccacccgc cgcttcgttg agcgttccgc cgccggcaaa
     3361 tcggccaagg tttaagcgca ggggcgacat ggccatacgc actggctgag gtacggcagc
     3421 cgcgaactct cgcgtcgtgg ccgggcttga ccccacggct atcacgctaa gcattctttc
     3481 tccgacccga tcgttccgga tagctggt
//
LOCUS       DASSZH010000106         2058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_377885, whole genome shotgun sequence.
ACCESSION   DASSZH010000106 DASSZH010000000
VERSION     DASSZH010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377885"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..1563)
                     /locus_tag="VFN42_02165"
     CDS             complement(109..1563)
                     /locus_tag="VFN42_02165"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET8995450.1"
                     /translation="MDSAKRALLLSFSERYSAFIVAAASVTILSRLLTPRDIGLYSIG
                     AALTSVLAMFRDFGVATFLIQEPEITAEKFHTALSISVLTALTIAALIAAAAPFAAGF
                     YGEPGIASVMHITAATFLFTAFNSVALTWFRREMRYAVLYRMTMAGAVCGAATSVTLA
                     ALGFGYMALAWASLANVTGIFCASMLYWPRHFGYRFCLKHGYAIGAFGFYSMAGNLLG
                     EVASRCDDLIVGRIVGLAPLGLFSRANGLVTMGRETTLSAVMPVAMSVFAVSSRAGES
                     FRPAYLGGVELLTGVVWPVFAFIGLMALPIIHILFGSQWDASAGPAHLLAIAGAIGAL
                     TVLHYPVYQATGALRQRVQVHLVCTPVQILSMAGAAYFGLTAAAGAVAFSALVEFAVA
                     QRAVNRLIDGSFRDIARAVRKSAYVAVATAAGPLLILIEARPTPGFMWQPLAGAAIVA
                     AIAWPAAIFGFRHPLSRELVGALRYGRGLLARLA"
     gene            complement(1663..>2058)
                     /locus_tag="VFN42_02170"
     CDS             complement(1663..>2058)
                     /locus_tag="VFN42_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995451.1"
                     /translation="ARSPDAYRTEYDAVICTEVLEHIEDDLSVIGNWSTGATCICSVP
                     NFLYQDQHVRAFRREAEVVARYGALLEISAIRRVAKPLIQSDGLGGYLRRLRWSRNDP
                     RKLMGLLGINRFANLAGWFVFCGRRRSGA"
BASE COUNT          412 a          719 c          643 g          284 t
ORIGIN      
        1 cggccggcat tcccccgctg ccgccggaag cctgcgccgg cctcgaccgg gccacccagt
       61 tcgcgcgcta cgaagccttc ctgcgcaccc agctccccca ggcctgaatc aggccagccg
      121 agcgagcagg ccacggccgt agcggagcgc cccaaccagc tcccgcgaca gcggatgccg
      181 aaaaccgaag atcgcggccg gccaggcaat ggccgcgaca atggccgcgc cggccagcgg
      241 ctgccacatg aagccgggcg tcggccgcgc ttcgatcagg atcagcagcg gaccggctgc
      301 ggtcgccacc gccacgtacg cgcttttccg gactgcccgg gcaatgtcgc ggaagctgcc
      361 gtcgatcagc cggttgaccg cgcgctgggc gaccgcgaat tcgaccaagg cactgaacgc
      421 cacggcaccc gccgccgcgg tcaggccgaa ataggccgcg cccgccatgc tgagaatctg
      481 cactggcgtg cagaccagat gcacctgcac gcgttgccgc agtgccccgg tcgcctgata
      541 gaccgggtaa tgcagcacgg tcagagcgcc gatcgccccg gcaatcgcca gcagatgcgc
      601 cggccccgcc gaagcatccc actggctgcc gaacaggatg tggatgatcg gcagcgccat
      661 caggccgatg aaggcgaaca ccggccagac cacgccggtc agcagctcca ccccgcccag
      721 ataggccggc cggaaactct cgcccgcccg cgaactcacc gcgaagaccg acatggcaac
      781 cggcatcacc gcgctgagcg tcgtctcccg ccccatggtg accaggccgt tcgcccggct
      841 gaacagaccc agcggcgcca gtccgacgat cctgcccacg ataaggtcat cgcagcggct
      901 ggcaacttcc cccagcaggt tgccggccat cgagtagaag ccgaatgcac cgatggcata
      961 gccgtgcttc aggcagaacc ggtaaccgaa atggcgcggc cagtacagca tgctggcgca
     1021 gaagatacct gtgacattgg ccagggaagc ccaggccagc gccatgtagc cgaagccgag
     1081 cgccgccagg gttaccgagg tcgccgcccc gcaaaccgca cccgccatcg tcatccggta
     1141 cagcaccgca tagcgcattt ctcgccggaa ccacgtcagg gccaccgaat tgaacgcggt
     1201 gaacaggaac gtagccgcag tgatgtgcat gacgctggcg atgccgggct caccgtagaa
     1261 gccggcagca aacggtgctg ccgcggcgat gagggcggcg atcgtcaatg cggtcagcac
     1321 gctgatactc agcgcggtgt ggaacttctc cgcggtgatc tccggttcct ggatcaggaa
     1381 cgttgcgaca ccgaagtcac ggaacatcgc caggaccgac gtcagcgcgg caccgatgga
     1441 atacagcccg atgtcgcgcg gagtcagcag gcgcgacagt atggtcaccg atgctgcggc
     1501 gactatgaag gccgaatacc gttcactgaa ggagagaagc agcgctctct tcgctgaatc
     1561 cattggctgt cacgcgtcgc ccaggccggt caggatcggg cgactagcat ttccgccgga
     1621 ggcggtcgat gcctttgtac ccggcatgcc ggtgcgccac gatcaggcgc cggaacgccg
     1681 ccgcccgcaa aacacgaacc agccggccag attggcgaaa cgattgatgc caagcagtcc
     1741 catcagcttg cgcggatcat tgcggctcca gcgtagccgg cgcaggtagc cgccaagccc
     1801 gtcggactgg atcaacggtt tggcgacccg gcggatggcc gagatctcga gcaatgcgcc
     1861 ataacgggcc acaacttccg cttcccggcg gaacgcgcgg acatgctggt cctggtaaag
     1921 gaaattcggc acgctgcaaa tgcatgtcgc accagtactc cagttgccga tcaccgatag
     1981 gtcgtcttcg atatgctcga gcacctcggt gcagatcacc gcgtcgtact ccgttctgta
     2041 ggcatccggc gaccgggc
//
LOCUS       DASSZH010000107         2628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_224497, whole genome shotgun sequence.
ACCESSION   DASSZH010000107 DASSZH010000000
VERSION     DASSZH010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224497"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="VFN42_02175"
     CDS             <1..1032
                     /locus_tag="VFN42_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET8995452.1"
                     /translation="VGDSPAMRHVSELIRRFTRTDEPVLVTGESGTGKELVARAIHQS
                     SSRRNGPFVAVNCAAIPANLVASELFGSEKGAFTGAVARTKGQIEHAHGGTLFLDEIG
                     DMPIDLQGHLLRFLQEGQIVRVGGRETINVNVRVVSATNVRLREAIAEGRFREDLYYR
                     LNVLMLPLPPLRERPEDIELLARHFLQEAARDFGRQVIDFDPEALAALRASPWPGNVR
                     ELMSVVRRAVVIGDGDHVSRTDLMGLEDSLRPAAAPRTAPTQPQPAAPPLLRPGSEEE
                     RQALLDALAHTQENVTLTAQELGVSRVTLYRMLRRHAISLSRGLREAPVGSRRPAAEG
                     EAAEDSRTA"
     gene            complement(1019..1366)
                     /locus_tag="VFN42_02180"
     CDS             complement(1019..1366)
                     /locus_tag="VFN42_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995453.1"
                     /translation="MEFGHAHAVLASQDRGFGGLSQVERRVLARWLTAARRAGIDTAE
                     DLGVRPWPAPSADSIIGVFKAGHLLASWLIVGQSGSWAVACCADGNVSAPLDTLADAL
                     ALVCPVEAPVRLS"
     gene            1691..1939
                     /locus_tag="VFN42_02185"
     CDS             1691..1939
                     /locus_tag="VFN42_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995454.1"
                     /translation="MLYEAREQCPNSVQNSEAENTGSEHQQQRLPTRAADAPDLLLFL
                     ERGHRAPALGETCRTQNVRAQLLHGLARVRRNGMKLVS"
     gene            2094..>2628
                     /locus_tag="VFN42_02190"
     CDS             2094..>2628
                     /locus_tag="VFN42_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HET8995455.1"
                     /translation="MSISETSSSAAPHRTPPAPSLVRALDRAGAFETACAQLMQIALR
                     DGRPDALIGIRTGGLVVAEAMASALDEPLPVLPLTSRRAGTAAKSRIKLLPKLLKHAP
                     RPVVDRLRVAELRLLSQRRRRSVQQQHVDPQEAEAIRAYLTRLPPGRTALVVDDAVDS
                     GVTLAAVLDALRQACRGD"
BASE COUNT          426 a          893 c          895 g          414 t
ORIGIN      
        1 gtcggcgaca gcccggcaat gcggcatgtc tccgagctga tccggcgctt cacccgcacc
       61 gacgagccgg tgctggtcac cggcgagagc ggcaccggca aggaactggt ggcgcgcgcg
      121 atccatcaaa gttcgtcgcg ccgcaacggt cctttcgtgg cggtgaactg cgcggcgatt
      181 ccggccaacc tggttgcgtc cgaattattc ggctccgaaa agggagcgtt caccggcgcc
      241 gtcgcgcgca ccaagggaca gatcgaacac gcgcatggcg gcacgctgtt cctggacgag
      301 atcggcgaca tgccgatcga tctgcaggga catctgctgc gcttcctgca ggaagggcag
      361 atcgtgcggg tcggcggccg cgaaacgatc aacgtgaacg tgcgtgtcgt ctccgccacc
      421 aacgtgcgcc tgcgcgaggc gatcgccgaa gggcggttcc gcgaggacct gtactatcgg
      481 ctgaatgtgc tgatgctgcc gctgccgccg ctgcgcgaac ggccggagga tatcgagctg
      541 ctggcccgcc acttcctgca ggaagcggcg cgtgatttcg gccggcaggt catcgacttc
      601 gatcccgagg cactggccgc gctacgggcc agcccgtggc ccggcaacgt gcgcgagctg
      661 atgtcggtgg tgcggcgcgc ggtggtgatc ggcgacggcg accacgtgag ccgcaccgac
      721 ctgatggggc tggaggattc gctgcgccct gccgcggcgc cgcgcaccgc gccgacgcag
      781 ccgcagccgg cggcgccacc gctgctgcgg cccgggtccg aggaagaacg ccaggccctg
      841 ctggacgcgc tcgcgcatac gcaggagaac gtcaccctga cggcgcagga actgggggtt
      901 tcgcgggtga cgctgtaccg catgttgcgg cggcatgcga tttcactgag tcgcggcctg
      961 cgcgaggcgc ccgtcggcag ccggcgcccc gccgccgagg gcgaggcggc ggaggattct
     1021 aggacagcct gaccggcgcc tcgacggggc ataccaatgc caacgcatcg gccagggtgt
     1081 cgagcggcgc cgagacattg ccatcggcgc agcaggcgac cgcccagctg ccggactgcc
     1141 cgacgatgag ccaggaggcc agcaggtgtc cggccttgaa cacgccgatg atgctgtctg
     1201 ccgagggcgc cggccagggc cggactccaa gatcctccgc ggtgtcgatc cctgcgcggc
     1261 gtgccgcggt gagccaacgc gccagcacgc gccgctcgac ctggctcaac ccgccgaaac
     1321 cgcgatcttg agaagccagc acggcgtgag cgtgtccaaa ctccattgtg gtctcgtcac
     1381 tcgcacaggt cggctgcgct gtccgacggg tcacctgatc tggcgtcatg gagtgcgcaa
     1441 tccccagcct cgacatgcat gtcacggctg caagctgcgt gccaggatca ctgttttttg
     1501 tatgcaatct tctccgaccg cacaggggcg ccggcacctg cggcaaaccc ggcttttcct
     1561 gtcatttgcg gcaataatcc gcgaggtgtc cggggcgcca tccagtgttc accaacgatc
     1621 cgtggtgacg tggcgtggta cgatacactg cgtcacatga ctcgcgacgt cacacacgca
     1681 tcgttccgac atgctatacg aagcgcgcga acaatgtcca aattccgtac agaacagcga
     1741 ggctgagaac actggatccg agcatcagca gcagcgcctg ccgacgcgcg cggcggacgc
     1801 gccggacctc ctgctgttcc tcgaacgcgg tcatcgcgcc ccagccctgg gcgaaacctg
     1861 ccggacgcag aatgtccgtg cgcagctgct gcacggtctg gcgcgcgtgc gccgaaacgg
     1921 gatgaagctc gtttcctgac atggcggaat gttcctgacc aggcgcctat ctctgttctg
     1981 caactgatcg ggcgcaaact ccagcttttg ccgctggatt attcgcagcc tcacccgaac
     2041 gtgaccacgg acactaccag acaggtcctg tggtttgcat cgaatccggg gccatgtcca
     2101 tcagtgaaac gtcctcgtcc gccgcgccgc accgcacgcc gccggcgccc tccctggttc
     2161 gtgccctgga tcgcgccggc gcgttcgaaa ccgcctgcgc ccagttgatg caaatcgcgc
     2221 tgcgcgatgg caggccggat gcgctgatcg gcatccgcac cggcggactg gtcgtggccg
     2281 aggccatggc cagcgcgctc gacgagcccc ttccggtcct gccgctgacc tcccgccggg
     2341 ccggcacggc ggcgaagtcg cgcatcaagc tgttgccgaa actgttgaag catgcaccgc
     2401 gcccggtggt ggatcggctg cgcgtcgccg agctgcggct gctgtcgcaa cggcggcggc
     2461 ggagcgtgca gcagcagcat gtcgatccgc aggaggcgga ggccatccgc gcctacctga
     2521 cccggctgcc gcccgggcgg acggcgctgg tggtggatga tgcggtggac agcggcgtta
     2581 ccctggcggc ggtgctggac gcgctgcgcc aggcctgccg cggcgatg
//
LOCUS       DASSZH010000108         2652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1298425, whole genome shotgun sequence.
ACCESSION   DASSZH010000108 DASSZH010000000
VERSION     DASSZH010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298425"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="VFN42_02195"
     CDS             complement(<1..879)
                     /locus_tag="VFN42_02195"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HET8995456.1"
                     /translation="MNVAVKPSPAAGTLLAEPPEIARVGVVGAGQMGNGIAHVCALAG
                     LPVILLDVKQDALDRAMAVMSRNMDRQVNRTLISAEDKADALARITTTTDYASFGDAD
                     LVVEAATEKEEVKRAVFKTLTPHLKPSCLLASNTSSISITRLGAATDRPEKFIGMHFM
                     NPVPVMKLVEIIRGIATDEPTFQAVQALAQKLGKIPAVAEDFPAFIVNRILVPMINEA
                     VYALYEGVGSVSGIDTSMRLGANHPMGPLELADFIGLDTCLSIMQVLYDGLSDSKYRP
                     CPLLVKYVEAVWLGRKT"
     gene            complement(880..1815)
                     /locus_tag="VFN42_02200"
     CDS             complement(880..1815)
                     /locus_tag="VFN42_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014105738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET8995457.1"
                     /translation="MTALVLLEFDDAGVKQPSRSAVAAAQKLGDVHVLVAGANLNAAA
                     GAAAKLPGVAKVLVADDARLDHLLAEPTAALLVALAPSYDHLLAATTAVGKNVMPRVA
                     ALLDAQPISDIAEVVDADTFVRPIYAGNGMATVKSSDAKKVITVRAASFDPVAAEGGT
                     APIEKAAIPELPDVSRFVSAELSKSERPELTAARVVISGGRGMQNGDNFKLLEPIADK
                     LGAAVGASRAAVDAGFVPNDYQVGQTGKIVAPDLYVAVGISGAIQHLAGMKDSKVIVA
                     INKDEEAPIFQVADYGLVADLFTVLPELAAELERS"
     gene            complement(1818..2567)
                     /locus_tag="VFN42_02205"
     CDS             complement(1818..2567)
                     /locus_tag="VFN42_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HET8995458.1"
                     /translation="MKVLVPVKRVVDYNVKVRVKADGSGVETAGVKMSMNPFDEIGVE
                     EAVRLKEKGVATEIIAVSLGVAQCQETIRTALAMGADRGILVETDAELQPLAVAKLLK
                     AIADQEQPTLIILGKQAIDDDMSATGPMLAALLGWPQATFASKVTIEGEAITVTREVD
                     GGLETVALKLPAIVTTDLRLNEPRYASLPNIMKARKKPIATMKPADLGVDPAPRLTTL
                     KVSEPPKRKAGVKVGSVAELVQKLKTEAKVI"
BASE COUNT          412 a          947 c          830 g          463 t
ORIGIN      
        1 ggtcttccga cccagccaga cggcttccac gtatttcacc agcagcgggc acggccggta
       61 tttcgaatcc gacagcccgt catacagcac ctgcatgatc gacaggcagg tatccagccc
      121 gatgaagtcc gccagctcca gcggtcccat tggatggttg gcgcccagcc gcatcgacgt
      181 gtcgatgccg gacaccgagc cgacgccttc gtacagcgcg tacaccgcct cgttgatcat
      241 cggcaccagg atgcggttga cgatgaacgc cgggaagtcc tccgccaccg ccgggatctt
      301 gcccagcttc tgcgccagcg cctgcaccgc ctggaaggtc ggctcatcgg tggcgatgcc
      361 gcggatgatc tccaccagct tcatcacagg caccggattc atgaagtgca tgccgatgaa
      421 cttttccggc cgatcggtcg ccgcgcccag ccgggtgatc gagatcgacg acgtattcga
      481 cgccagcagg caggacggct tcaggtgcgg cgtcagcgtc ttgaagaccg cccgcttcac
      541 ctcctccttc tcggtcgccg cctccaccac caggtcggcg tcgccgaacg acgcgtaatc
      601 ggttgtggtg gtgatccgcg ccagcgcgtc ggccttgtcc tcagcggaga tcagggtacg
      661 gttcacctgc cggtccatgt tccggctcat gacggccatc gcgcggtcaa gcgcgtcctg
      721 cttcacatcc agcaggatca ccggcaggcc ggccaacgcg cagacatgcg cgatgccgtt
      781 gcccatctgg ccggcgccca ccacgccgac ccgggcgatc tccggcggtt cggccagcag
      841 ggtgccggcg gcaggggacg gcttgactgc gacgttcatt caggagcgct ccaactcggc
      901 cgccagctcg ggcagcacgg tgaacaggtc agccaccagg ccgtaatcgg cgacctggaa
      961 gatgggcgct tcctcgtcct tgttgatcgc cacgatcacc ttgctgtcct tcatcccggc
     1021 gaggtgctgg atggcacccg agatgccgac ggcaacgtac aggtccgggg cgacgatctt
     1081 tccggtctgc ccaacctggt agtcgttggg cacgaagccg gcatccaccg cggcgcggga
     1141 agccccgacc gcggcgccca gtttatcagc gataggttcc aaaagcttaa aattgtcgcc
     1201 gttctgcatg ccacggccgc cggaaatcac cacccgggcc gcggtcagct ccggccgctc
     1261 gctcttcgac agctcggcgg agacgaaacg cgacacgtcg ggcagctccg ggatggcggc
     1321 cttttcgatc ggcgcggtgc cgccttcggc cgccaccggg tcgaaactgg cggcgcgcac
     1381 ggtgatcacc ttcttcgcgt cggacgactt caccgtcgcc atgccgttgc cggcatagat
     1441 cggccgcacg aaggtgtcgg cgtccaccac ctcggcgata tcgctgatcg gctgtgcatc
     1501 cagcagcgcg gccacccgcg gcatcacgtt cttgcccacc gcggtggtgg ccgccagcag
     1561 gtgatcgtag gaaggcgcca gcgccaccag cagcgcggcg gtcggctccg ccagcaggtg
     1621 gtccagccgc gcgtcgtccg ccaccagcac cttggccacg ccgggcagct tggccgccgc
     1681 gccggcggcc gcgttcaggt tcgccccggc gaccagcacg tgcacgtcgc ccagcttctg
     1741 cgccgcggcc actgcgctgc gcgacggctg cttcacgccg gcatcgtcga attcgagcag
     1801 gacaagagcc gtcatcgtca gatcaccttc gcttcggtct tcagcttctg caccagctcg
     1861 gccaccgagc cgaccttcac ccccgccttg cgcttcggcg gctcgctgac cttcagcgtg
     1921 gtcagccgcg gcgccggatc gacgcccaga tccgccggct tcatcgtggc gatcggcttc
     1981 ttgcgcgcct tcatgatgtt cggcagcgag gcgtagcgcg gctcgttcag ccgcagatcg
     2041 gtggtgacga tcgccggcag cttcagcgcc accgtctcca gcccgccatc cacttcgcgg
     2101 gtgacggtga tcgcctcacc ctcgatggtg accttgctgg cgaaggtcgc ctgcggccag
     2161 ccgagcaggg cggcgagcat ggggccggtg gccgacatgt cgtcgtcgat cgcctgcttg
     2221 cccaggatga tcagcgtcgg ctgctcctgg tcggcgatcg ccttcagcag cttggccacg
     2281 gccagcggct gcagctccgc gtcggtctcc accaggatgc cgcggtcggc gcccatggcc
     2341 agcgcggtgc ggatggtctc ctggcactgg gcgacgccca gggagacggc gatgatctcg
     2401 gtggcgacgc ccttctcctt caggcggacc gcctcctcga cgccgatttc gtcgaacgga
     2461 ttcatggaca tcttcacgcc ggcggtttcc acaccggaac cgtccgcctt gacgcggacc
     2521 ttgacgttgt aatcgaccac ccgctttacc ggcactaaga ccttcatcga tcctctttca
     2581 ctcaatttgg gaggggggtt atcaactggc gcggaaccta aaggcccgat ctgccccggt
     2641 caacgcgcga gc
//
LOCUS       DASSZH010000109         5262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_616729, whole genome shotgun sequence.
ACCESSION   DASSZH010000109 DASSZH010000000
VERSION     DASSZH010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5262
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616729"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VFN42_02210"
     CDS             <1..796
                     /locus_tag="VFN42_02210"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET8995459.1"
                     /translation="LVRALRAGNFDRAVLLHHSHTLAFATLAAGIRVRQGYGFGAQRW
                     LLNRGPYLPDPARRMHQLQRATAFLRAAEVPLADPEPRMLVTRAARDAVAGRLAGVRR
                     PLVAMGIGSSEAHRQWGAERMAALGGALLKAGWGSLALIGGPDDGALVTAIQAGLGDN
                     AGRTVPALGWHLAETAALIAAAEFYVGNDTGMMNLAAATGVRSYGLFGASPPLGHSRQ
                     IVAVTPPDRQQSPTGMRRISPDAVLAVIQADRGGLAPRPDQVQSQP"
     gene            complement(775..1014)
                     /locus_tag="VFN42_02215"
     CDS             complement(775..1014)
                     /locus_tag="VFN42_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019090155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995460.1"
                     /translation="MAQEISLIARRCERAVITAYRDLRHLGADDLAAFQACTTLYRIH
                     HPESSVNEARRLVAEWIDHHVVRGDRGPTQGCDCT"
     gene            1287..1550
                     /locus_tag="VFN42_02220"
     CDS             1287..1550
                     /locus_tag="VFN42_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995461.1"
                     /translation="MPQISDQAKPATAGTVPSSAERRRPGRPEAVNPALIPLLRRDHL
                     LNDVLAQRDGDDLGPARGIGFSAMLGLVLWVATAALGWWLFAR"
     gene            1621..2193
                     /locus_tag="VFN42_02225"
     CDS             1621..2193
                     /locus_tag="VFN42_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3035 domain-containing protein"
                     /protein_id="HET8995462.1"
                     /translation="MRTQKFYGRLTAALLLAGTTVLLAGCGNSDLSRTFGFTRDSPDE
                     FSVTKQAPLSMPPDFALRPPRPGESRPQDLSETRQAQEALVPQAALGTPQASLSPGQQ
                     ALVQEAGPPAPRGIRQDLRQDSEKREAANDSFVNKLLFWRQPAPGSAVVDPKREAQRL
                     RQNSALGESPETGHTPIIQPHQKSWLDSLF"
     gene            2447..>5262
                     /locus_tag="VFN42_02230"
     CDS             2447..>5262
                     /locus_tag="VFN42_02230"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET8995463.1"
                     /translation="MSTPFSSQDLGRIFDGRTLTRGRSLGLAGAVTVELEGDTIAASV
                     QERSARHRVRITPSRLGRRVVFDHSCSCGVRACAHIAAAAFAALDRFPELRRPEQQTF
                     FDSLVETAAPETEAQRPAFELSPGEAPYACMVATLLIGQRSGTVAPTTPAAIEADMLA
                     GTATREAARAIGGSRTRTGIVTAEVDSVLNALLRSGQARWHAGGRKLIAGQPRIVGSP
                     AEMVLPPRSGIIAGSGGRWYIDAETGAVGRVRIRPPIARPQPVPPPPASEPRRRFEAP
                     AATEQVIVTRPFSPVLRLTRFPCPDAFARMQTLDGLLVEFDYGGAVVAFDDDRQFVRA
                     ETPAGPVFVRRDAAGEGEVLEAIRRDGLVQMRMATGGAAKGRMVFVFRGRDAAESWQQ
                     FITERLPALESMGWRNRVDEDFGPRLVESVGDCDLRVADAPGGRFSLDLGIEIDGERQ
                     PLLPILLRLRERGGISAARITDGALITSLDDGRILKLPADRITRLLALLDDLIESAAR
                     SNGETLELESGEASSVIDLEDLVTTRWQDGAAIARHVARFRSAADIPPVSVPQEFTAT
                     LRPYQQQGVNWLQHLRASELGGLLADDMGLGKTAQTIAHIAVEHAEGRLDRPALVVVP
                     TSLVPNWTAELARFAPHLRVVVLHGLDRHEKRRELGSVHVAITTYTVLARDIDEMAQI
                     AWHLVVLDEAQAIKSPAAKATHAVCRLETRHRLCLSGTPIENNLGELWSQFAFLMPGL
                     LGQRKHFSRRFRTPIEKENDPVRRRQLATRIRPFILRRTKAAVATELPPKHTILRRIT
                     LAPEQRELYETIRATLHDKVRQEIADRGLGRSHVVVLDALLKLRQVCCDPRLVKLPSA
                     RLAGVSSKLDDLLEMLTEMIAEGRRILLFSQFTSMLDLIKPAVHAAGIPFVELRGDTA
                     DRAAPVQRFEAGEVPLFLISLKAGG"
BASE COUNT          786 a         1801 c         1856 g          819 t
ORIGIN      
        1 gctggtccgg gccctccgcg ccggcaattt cgatcgcgcg gtgctgctgc atcatagcca
       61 caccctggct ttcgcgacgc ttgccgcggg gattcgcgtc cgccagggct acggcttcgg
      121 cgcccaacgg tggctgctga accgtggtcc ctacctgccg gaccccgctc gccggatgca
      181 ccagttgcaa cgcgccaccg cgttcctgcg ggccgccgag gttccgcttg ccgacccgga
      241 gccgcgcatg ctggtgaccc gagccgcccg cgacgcggtc gccggccggc tggccggtgt
      301 ccgccgtccg ctggtggcga tgggcatcgg cagttcggag gcgcaccgcc agtggggagc
      361 agagcgcatg gcagcgctcg gcggcgcgct gctgaaggct gggtggggca gcctggcgct
      421 gatcggcggc ccggacgatg gcgcgctcgt cacggcgatc caggcaggtc tgggcgacaa
      481 cgccggccgc accgtgccgg cgctgggctg gcatctggcg gagaccgcgg cgctgatcgc
      541 cgcagcggaa ttttacgtgg gcaatgacac cgggatgatg aacctggccg ccgcgaccgg
      601 ggtccgcagc tacggcctgt tcggcgcctc accaccgctg ggccacagcc ggcagatcgt
      661 tgccgtcacc ccgccggacc ggcagcaatc acccaccggc atgcggcgta tctcgcctga
      721 cgcggtgctg gccgtgattc aggccgatcg gggcgggttg gcgccccggc cggatcaggt
      781 gcagtcacaa ccctgagtcg ggccacgatc gccgcgcacc acgtggtggt cgatccactc
      841 ggccaccagg cggcgcgcct cgttcaccga ggactccgga tgatggatac ggtagagcgt
      901 cgtgcacgcc tggaatgcgg ccaggtcgtc tgcacccagg tggcgcaggt cgcgataggc
      961 ggtgatcacg gcacgctcac agcgtcgggc gatcaggctg atctcttgcg ccattcgcgg
     1021 gactgctccc tctaattgcg aatcattcgc agtttctggg caaaacatag gcgctgccgg
     1081 cgggcctcgc aataagggac attacggaag gtaaaatccc acttggcatg gtagatttgt
     1141 aacaagcgcg atatctggcg atttggcgca gaaatcgcgc atgcattgcg cggttgcggc
     1201 gacacgatgc cgcgacccga cacaatgtta acacatcgga aactaattag tgcagactat
     1261 ccgaatgtag cagcagcagc ggaagcatgc cccagatcag cgatcaggcg aagccggcga
     1321 cggcgggaac ggtgcccagc tcggccgaac gtcgccgccc cggccggccc gaggcggtga
     1381 acccggcgct gatcccactg ctgcgccggg accatctgct gaacgacgtg ctggcgcaac
     1441 gcgacggcga cgatctcggc ccggcgcgcg gcatcgggtt cagcgccatg ctcgggctgg
     1501 tgctgtgggt cgccacagct gcgctgggct ggtggttgtt tgcccggtaa gcagccgcgg
     1561 cttcgggttg gcgaacgcgg cgcttgcggc gcgcccgggt ccgcctgtag gaagcccgac
     1621 atgcggacac aaaagtttta tggccggctg acggcggcgc tgctcctggc aggcacaacg
     1681 gtgctgctgg cagggtgcgg caatagcgac ctctcgcgga ccttcggctt cacccgcgac
     1741 tcgcccgacg aattctctgt caccaagcag gcgccgctat ccatgccgcc ggatttcgcc
     1801 ttgcgtccgc cccgtcccgg cgagtcgcgc ccgcaggact tgtcggagac gcggcaggcg
     1861 caggaggcgc tggtgccgca ggcggccctg ggtacgccgc aggcgagcct cagcccgggc
     1921 cagcaggccc tcgtccagga agcgggaccg ccggcccccc gaggcattcg gcaggatctc
     1981 cgccaggaca gcgagaagcg cgaggcggcc aacgacagct tcgtcaacaa gctgctgttc
     2041 tggcgccagc ccgcaccggg gtccgctgtg gtggacccca agcgtgaggc gcagcggctg
     2101 cggcagaatt ccgcacttgg cgaaagcccg gagaccggcc acacgccgat catccagccg
     2161 caccagaaga gctggctgga cagcctgttc tagccgccct tgcgcgccgg caccggcgcc
     2221 ttccgtggct tctcaggatg ctggccgacc cgccgaacgg gcgctcggcc ggcatgcagg
     2281 cgcatgtcaa cactgaccgc agcaccccgg atcactgccg agcgatggca atgcgggcgc
     2341 gctgatcccg gttgcggctg ctcggcctgc tcgccatggt ggcagggatc tcctgtcgat
     2401 tcgccgtccg ccgcactcct aaccgcctga gaatcctgcc cgccgtatgt cgacgccgtt
     2461 ttcctcccag gatcttggcc gcatcttcga cggtcgtacc ctcacccggg gccgcagcct
     2521 ggggctggcg ggtgccgtca ccgtcgagct cgagggtgac accatcgcgg cctcggtaca
     2581 ggaacgctct gcccggcatc gcgtgcgcat taccccgtcg cggctggggc gccgcgtcgt
     2641 gttcgaccac agctgctcct gcggggtgcg cgcttgtgcg catatcgccg cggccgcgtt
     2701 tgccgctctt gaccggtttc ccgaactgcg ccgcccggag cagcagacat ttttcgactc
     2761 gctggtcgaa accgcggcac cggaaacgga ggcccagcgc ccggcctttg agctgtcgcc
     2821 cggcgaggcc ccctatgcct gcatggtcgc gacgctgctg atcggccagc gcagcggcac
     2881 cgtcgccccg acgacgcctg cggcgatcga ggccgacatg cttgccggga ccgcgacccg
     2941 cgaggctgcg cgtgcgatcg gcggcagccg gaccaggacc ggcatcgtga cagcggaggt
     3001 ggacagcgtg ctcaacgccc tgctgcgcag cggccaggcc cgctggcacg ccggggggcg
     3061 caagctgatt gcgggccagc cgcgtatcgt gggttcgccc gccgagatgg tgctgccgcc
     3121 gcgctccggc atcatcgccg gcagtggcgg tcgctggtac atcgatgcgg aaaccggcgc
     3181 ggtcggccgg gtgcgcatcc gcccgccgat cgcgcgcccg cagcccgtgc caccgccgcc
     3241 ggccagtgaa ccacgccggc ggttcgaggc gcccgccgcc accgagcagg tgatcgtcac
     3301 ccggccgttc agtcccgtgc tgcggctgac gcgctttccc tgccctgacg cgtttgcgcg
     3361 catgcagacg ctggatgggc tgctggtgga attcgactat ggcggcgcgg tcgtggcgtt
     3421 cgatgacgat cggcagttcg tgcgcgccga gacgccggcg ggcccggtct tcgtgcgccg
     3481 cgatgcggca ggggagggcg aggtgctcga agccatccgc cgcgacggcc tggtgcagat
     3541 gcgcatggcc accggcgggg cggcgaaggg ccgcatggtg ttcgtgttcc gcggccgcga
     3601 cgccgccgag tcgtggcagc agttcatcac cgaacgcctg ccggccctgg aaagcatggg
     3661 ctggcgcaac cgggtggacg aggatttcgg cccgcggctg gtcgagtcgg tcggcgattg
     3721 cgaccttcgg gtggccgatg cgccgggcgg acggttctcg ctggacctgg gtatagagat
     3781 cgacggcgaa cgccagccgc tgctgccaat tctgctgcgc ctgcgtgaac gcggcggcat
     3841 atccgcggcg cgcatcaccg atggtgcgct gatcaccagc ctggacgacg gccgcatcct
     3901 gaagctgccc gccgaccgta tcacccggct gctggcgctg ctggacgatc tgatcgagtc
     3961 cgccgcgcgt tccaacggcg agacgctgga actggaatcc ggcgaggcgt ccagcgtcat
     4021 cgacctggag gacctggtca ccacgcgctg gcaggatggc gcggcgatcg cccggcacgt
     4081 ggcgcgcttc cggtcggcgg ccgatatccc gccggtcagc gtgccgcaag agttcaccgc
     4141 cacgctgcgg ccgtaccagc agcagggcgt gaactggttg cagcatctgc gcgccagcga
     4201 actcggtggc ttgctggccg acgacatggg gctgggcaag accgcgcaga ccatcgctca
     4261 tatcgccgtc gagcatgccg aggggcggtt ggaccggccg gcgctggtgg tggtgccgac
     4321 cagcctggtg cccaattgga ccgcggaact cgcgcgtttc gccccgcatc tgcgcgtggt
     4381 ggtactgcac gggctggacc gccacgagaa gcggcgcgag ctgggtagcg tgcatgtggc
     4441 gatcactacc tacaccgtgc tggcgcgcga tatcgatgag atggcgcaga tcgcctggca
     4501 cctggtggtg ctggacgagg cacaggcaat caagagccct gccgccaagg cgacgcatgc
     4561 ggtgtgccgg ctggagacgc ggcatcggct gtgcctgtcg ggaacgccga tcgagaacaa
     4621 cctgggcgag ctgtggtcac agtttgcctt cctgatgcct ggcctgctgg ggcagcggaa
     4681 gcacttctcc cgccgcttcc gcacgccgat cgagaaggag aacgacccgg tgcgccgccg
     4741 ccagcttgcg acgcgtatcc ggcccttcat cctgcgccgc accaaggcgg cggtggcgac
     4801 ggaactgccg ccgaagcaca ccattctgcg ccgcatcacc ctggcgccgg aacagcgcga
     4861 actgtacgaa accatccgcg ccacgctgca cgacaaggtg cggcaggaga tcgccgatcg
     4921 tggcctcgga cgcagccatg tcgtggtgct ggatgcgctg ctgaagctcc gtcaggtgtg
     4981 ctgcgatccg cggctggtga agctgccctc cgcgcggctg gccggtgtct ccagcaagct
     5041 ggacgacctg ctggagatgc tgaccgagat gatcgcggag gggcggcgga tcctgctgtt
     5101 ctcgcagttc acctccatgc tggatttgat caagccggca gtgcacgcgg caggaattcc
     5161 gtttgtggag ctgcgcggcg acaccgccga ccgtgccgcg ccggtgcagc gcttcgaagc
     5221 gggcgaggtc ccgctgttcc tgatcagcct gaaggcgggc gg
//
LOCUS       DASSZH010000110         2829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1042823, whole genome shotgun sequence.
ACCESSION   DASSZH010000110 DASSZH010000000
VERSION     DASSZH010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042823"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VFN42_02235"
     CDS             <1..807
                     /locus_tag="VFN42_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8995464.1"
                     /translation="GIHALPALALAAATFYAPPVAQAAPAIPGLTTAGTITFCSSISQ
                     PPFEFLDAQMQPKGLDVEIGDGIAKRLGLQVAWKNIPFSGLIPAIQANQCDAILSDLF
                     VKEERMRVLDFVPYMYSQEVLLLKKGTKGINGLSDLSGKKAATVTGTTATQLLEAASK
                     KLVAAGKPAINIVMFPENTQALQQVQFGQVQAFGVAYEIAEYYMQHAPDTFELGGPAY
                     FKILTGIGLRKNEHALRDAMQTAFDAMRKDGSYEKILAAWHLESDSLAKK"
     gene            814..1590
                     /locus_tag="VFN42_02240"
     CDS             814..1590
                     /locus_tag="VFN42_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET8995465.1"
                     /translation="MVFNWDYFARQLFLPGGLFMHGLLLTVTVSLVSQLLGSALGLLV
                     ALGRISKWPAVRTGCGLYIWLIRGTPLLVQIVFIYTGLAAANLFHFQDIALGPLVIPA
                     NVQAGILALSLNEGAYMAEIIRAGIAGVDFGQAEAARALGMPPLLVMRRIVLPQAFRL
                     IIPPVGNQFNIMLKNSTLLSIIGVPELLLVTETINSATFRTFELYSVVGLYFLALTTA
                     WSLVQRAIERRFTAPGRHAVRDRRARRRLLAFTQAQEGGL"
     gene            1587..2375
                     /locus_tag="VFN42_02245"
     CDS             1587..2375
                     /locus_tag="VFN42_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET8995466.1"
                     /translation="MSATVATAEPAVVQAVNIHKWFGETKVLDGVSLDVGRGETVVLI
                     GSSGSGKTTFLRCLNHLETIDRGRISVNGATIGYRERNGGLVPDRERSVARHRREIGM
                     VFQRFNLFQHMTALENIIEAPVHVRGVPKAEAVDTARGLLARVGLADKADNYPVQLSG
                     GQQQRVAIARALAMAPQVLLFDEPTSALDPEMVNEVLEVIRGLARDGMTMVIVTHEMG
                     FARQVADRVVVMDAGRIVEFGTPEQVFTNPQHARTQLLMASILR"
     gene            complement(2453..>2829)
                     /locus_tag="VFN42_02250"
     CDS             complement(2453..>2829)
                     /locus_tag="VFN42_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019085136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET8995467.1"
                     /translation="AEVNEAKKVLATEATALAHGRDPAEAAAETARRTFEEGESADSL
                     PGVDVPRGELAAGIPAFRLFTLAELAKSNGEARRLIRGGGARINDAPVTDEGQMVSLA
                     DLQDGAIKLSAGRKHHRLVRAA"
BASE COUNT          465 a          931 c          951 g          482 t
ORIGIN      
        1 ggcatccatg ctctgccggc gctcgccctt gccgccgcga ccttctacgc accgcccgtt
       61 gctcaggcgg cgccggccat ccccggcctg accaccgccg gcacgataac cttctgcagc
      121 tccatctccc agccgccctt cgaattcctc gacgcgcaga tgcagccgaa gggcctcgac
      181 gtagaaatcg gcgatggcat cgccaagcgc ctcggcctgc aggtggcatg gaagaacatc
      241 ccgttcagcg ggttgatccc ggcgatccag gccaaccagt gtgacgcgat cctctccgac
      301 ctgttcgtca aggaggagcg catgcgcgtg ctcgatttcg tgccctacat gtattcgcag
      361 gaagtgctgc tgctgaagaa gggcaccaag ggaatcaacg ggctgtccga cctgtcgggc
      421 aagaaggccg ccacggtcac cggcaccacc gcgacgcagc tgctggaggc ggcgagcaag
      481 aagctggtcg ccgcgggcaa gccggcgatc aatatcgtga tgttcccgga gaacacccag
      541 gcgctgcagc aggtgcagtt cggccaggtg caggcgttcg gcgtcgctta tgagatcgcc
      601 gagtactaca tgcagcacgc gcccgatacg ttcgaactcg gcggtccggc ctatttcaag
      661 atcctcaccg gcattggcct gcgcaagaac gagcacgcgc tgcgtgacgc catgcagacg
      721 gcgttcgacg ccatgcgcaa ggacggcagc tacgagaaga tcctcgccgc ctggcatctg
      781 gaaagcgact ccctggcgaa gaagtaggcg gctgtggtct tcaactggga ttacttcgcc
      841 cggcagttgt tcctgccggg cgggctcttc atgcacgggc tgctgctgac cgtgacggtc
      901 agcctggtct cgcagttgct gggcagcgca ctcggactgt tggtggcgct cggccgcatt
      961 tccaaatggc cggcggtgcg caccggctgc ggcctgtata tctggctgat ccgcggcacg
     1021 ccgctgctgg tgcagatcgt gttcatctac accggcctgg ccgcggcgaa cctgttccac
     1081 ttccaggaca tcgcccttgg cccgttggtg ataccggcca atgtacaggc cggcatcctg
     1141 gcgctgagcc tgaacgaagg cgcctacatg gctgagatca tccgcgccgg catcgccggg
     1201 gtggatttcg gccaggccga agcggcacgc gcgctgggca tgccgccgct cctggtgatg
     1261 cgccgcatcg tgctgccgca ggcctttcgc ctgatcatcc cgccggtcgg caaccagttc
     1321 aacatcatgc tgaagaacag cactctgctg agcatcatcg gcgtgccgga actgctgctg
     1381 gtgacggaga cgatcaactc cgccacgttc cgcaccttcg agctgtactc ggtggtgggc
     1441 ctgtacttcc tggcgctgac cacggcatgg agcctggtgc aacgggcgat cgaacggcgc
     1501 tttaccgcgc ccggccggca tgccgtgcgg gatcgccgcg cgcggcgacg cctgctggca
     1561 ttcacccagg cacaggaggg cgggttgtga gcgcgactgt cgcaaccgcc gagccggcag
     1621 tggtgcaggc ggtcaatatc cacaagtggt tcggcgagac caaggtgctt gacggggtgt
     1681 cgcttgacgt cggccgcggc gagacggtgg tgctgatcgg ctcctccggt tccggcaaga
     1741 cgacgttcct gcgctgcctg aaccatctgg agacgatcga tcgtggccgc atcagcgtga
     1801 acggcgccac gatcggttat cgcgagcgca atggcgggct ggtgcccgac cgcgagcgca
     1861 gcgtcgcgcg ccaccggcgc gagatcggca tggtgttcca gcgcttcaac ctgttccagc
     1921 acatgacggc gctggagaac atcatcgagg cgccggtgca tgtgcggggc gtgccgaagg
     1981 ccgaggcggt cgataccgca cgcggcctgc tggcgcgggt cgggctggcc gacaaggcgg
     2041 acaactatcc ggtgcaactg tcgggaggcc agcagcagcg cgtcgccatt gcgcgcgcgt
     2101 tggcgatggc gccgcaggtg ctgctgttcg acgaaccgac cagcgcgctc gatccggaga
     2161 tggtgaacga ggtgctggaa gtgatccgag gcctggcgcg ggacggcatg accatggtga
     2221 tcgtcacgca tgagatgggc ttcgcgcgcc aggtggcgga cagggtggtg gtgatggacg
     2281 ccggccgcat tgtggaattc ggcaccccag agcaggtctt caccaacccg cagcacgcgc
     2341 ggacgcagct gctgatggcg tcgatcctgc gttagcggac cggtacatcg gcggatcgga
     2401 gccgtcgccc gggcgtgccg tcatggccgg caagagccag tcgcgacgaa catcaggcag
     2461 cgcgcaccag acgatgatgc ttgcggccgg cggacagctt gatcgcaccg tcttgcagat
     2521 ccgccagcga caccatctgc ccttcatcgg tcaccggcgc atcgttgatg cgtgcgccgc
     2581 cgccgcggat cagccggcgg gcttctccgt tgctctttgc caactccgcc agggtgaaca
     2641 gccggaacgc cggaatgccc gccgcgagtt caccccgcgg cacgtcaacg ccgggcagcg
     2701 agtcggctga ttcgccttcc tcgaaggtgc ggcgcgcggt ctccgctgcg gcttccgctg
     2761 ggtcgcggcc atgcgccagc gcggtagctt cagtggcgag caccttcttc gcctcgttca
     2821 cctcggcac
//
LOCUS       DASSZH010000111         3004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1145085, whole genome shotgun sequence.
ACCESSION   DASSZH010000111 DASSZH010000000
VERSION     DASSZH010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145085"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1159
                     /locus_tag="VFN42_02255"
                     /pseudo
     CDS             1..1159
                     /locus_tag="VFN42_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005168.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
     gene            complement(1113..2216)
                     /locus_tag="VFN42_02260"
     CDS             complement(1113..2216)
                     /locus_tag="VFN42_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET8995468.1"
                     /translation="MLAIISAALFGASTPVAKLLLGQIDPWLLAGVLYLGSGIGLATV
                     HISRRSLGIVPPEAPLRRTDLPWLVAVVLAGGVLGPLLLMLGLSVTPASSAALLLNLE
                     GLATMAIAWLVFREHTDRRIVLGALLILSGAVLLSWQGEVAGVGWGALAILGACLAWG
                     ADNNLTRKVSAADPVQIAMIKGLAAGAVNTALALWFHARVPGAATLLAGAAVGFLGYG
                     VSLVLFVLGLRHLGSGRTAAYFSTAPFLGAVLALAIFREPPTVTLAAAAMLMAAGLWL
                     HLAEHHEHEHVHEPMVHEHAHVHDEHHQHTHASGAPPGEPHVHVHRHQRLVHRHPHYP
                     DLHHQHAHEAPAEEQGVRITPRSAPAAAARRSA"
     gene            complement(2287..2697)
                     /locus_tag="VFN42_02265"
     CDS             complement(2287..2697)
                     /locus_tag="VFN42_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding protein"
                     /protein_id="HET8995469.1"
                     /translation="MRSAGIRIGELSRRTGCSIETIRYYERIGLLPEPERTGRYRCYG
                     NGGVRRLTFVRRARELGFTLDEVRSLLTLSANDDGNACAAAQQLGAARLTEVRERIAD
                     LRIIERILAGAVQACNPEQRRGCPLIEKLSDQRH"
     gene            2779..>3004
                     /locus_tag="VFN42_02270"
     CDS             2779..>3004
                     /locus_tag="VFN42_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995470.1"
                     /translation="MGEPVDISQDRTTQGLGAVVLALGGLGAAFGVASCCGLPFLLAT
                     AGLGTAWLGGVALLAAPHRQFLLVAAAASLA"
BASE COUNT          446 a         1021 c         1083 g          454 t
ORIGIN      
        1 acgccggccg ccgtcggtat cgcggcggtc gtggcggtgc tgccgccgct cgccctgggc
       61 ctgccctggg aaacctggac ctatcgcggc ctggcgctgc tgctgatcgc ctgcccctgt
      121 gccctggtgc tctccacccc cgcggccatc gcctccggcc tggcggcggg cgcgcggcgc
      181 gggttgctgg tgaagggcgg cgcggcgctg gaagcgatcg cccgggtcgg caccgccgca
      241 ttcgacaaga ccggcacgct gaccgagggc cggccccgcg tcaccgacct gctcgccacc
      301 gccggcaccg agcgttccct gctcgccctg gccgccgcgg cggagaacgg ctccagccac
      361 ccagttgccc gggccatcct gacccgggcg gcggaggacg gcattccgct gcggcccgcc
      421 cgcaatgcgc gcgccattcc cggcagagcc gttgaggcgg tggtcaccgg caagcgggtt
      481 ggcgtcggtt cacccgccta tgcgtccgaa caggggccga tggcggcgga actggcggag
      541 cgcgtcgctg cctgggaagg gcagggcaag acggtggtcg ccgtcgtggt ggacggtgca
      601 acggccgggc tgatcgcggt gcgtgacgag ccgcgccgcg atgccgccgc cggcatccgg
      661 caattgcagg cactgggcgt gcaggccgtc atgctgtccg gcgacaaccg gcgcaccgcc
      721 gaggcggtgc gccgccagcg tcgggatgaa ggccgtcgcg ggcctgctgc ctgacgacaa
      781 ggtgcgggag atcactgctt tgcgcgcgcg cgggccggtc gtcatggttg gcgacggcat
      841 caacgacgcg ccgtcgctgg cgaccgcgga tgtcggcatc gccatgggcg gcggcaccga
      901 cgtggcgctg gagaccggcg acgccgcgat cctgcacgac cgggtcgccg atgtggcggc
      961 cctggtgcgg ctcgcccggg caacgctggc caacatccgg cagaacgtga cagtggcgct
     1021 ggggctgaaa gggatcttcc tggtgaccac cattgccggt ttcaccggcc tgtggcccgc
     1081 ggtgatggcg gacaccggcg ccacggtgct ggtcacgctg aacgccttgc ggctgctgcg
     1141 ggtgcgctgc ggggagtgat cctcaccccc tgctcttcgg cgggtgcttc gtgcgcgtgc
     1201 tggtgatgca ggtccggata gtgcggatgc cggtgcacca gcctttggtg ccgatgcacg
     1261 tgcacatgcg gttcgcccgg tggagcgccc gacgcatgcg tgtgctggtg gtgttcgtcg
     1321 tgcacatggg cgtgctcgtg caccatcggc tcatgcacgt gctcatgctc gtggtgttcg
     1381 gccaggtgca gccaaagtcc cgccgccatc agcatggcgg cggcagccag ggtcacggtg
     1441 ggcggttcgc ggaagatggc cagcgccagc accgcaccca ggaacggcgc ggtggaaaaa
     1501 tacgccgcgg tccggcccga cccgagatgc cgcaacccca gcacgaacag caccaggctg
     1561 acgccatagc cgaggaagcc aaccgcggcc ccggcgagca gcgttgcggc gccgggcacg
     1621 cgcgcgtgga accacagcgc gagcgccgtg ttgaccgcgc cggccgcgag tcccttgatc
     1681 atcgcgatct gcactgggtc ggccgcgctg accttgcgcg tcaggttgtt gtcggccccc
     1741 caggccaggc aggcgccgag gatggccagc gcgccccagc cgacacccgc cacctcgccc
     1801 tgccaggaca gcagaacggc gccgctcagg atcagcagtg cgcccagcac gatccggcgg
     1861 tcggtgtgct cgcggaacac cagccaggcg atcgccatcg tggcgaggcc ctccaggttc
     1921 agcagcaggg cggcgctgga agccggcgtc acgctgaggc cgagcatcag cagcaacgga
     1981 ccgagcacgc cgccagccaa cacgacggcg acgagccagg gcaggtcggt gcggcgcagc
     2041 ggcgcctcag gcggcacgat gcccaacgag cggcggctga tatgcacggt cgcaaggcca
     2101 atgccggagc ccagatacag cacgcccgcc agcagccagg gatcgatctg ccccagcagg
     2161 agttttgcaa ccggcgtgct ggcgccgaac aacgcggcgg agattatggc cagcatggca
     2221 ccaggggagg cgagggctgg gaacggcaat acagttcccc tttctcgtcg gaatcgctgc
     2281 cagctctcag tgccgctgat ccgagagttt ctcgatcaaa gggcagcccc gccgctgctc
     2341 cggattgcac gcctggaccg ctcccgcgag gatgcgctcg atgatacgca gatcggcaat
     2401 ccgttcgcgc acctctgtca ggcgtgcagc gccgagttgt tgcgctgcgg cgcaggcatt
     2461 tccgtcgtca ttggcagaca gcgtcagcag gcttcgcacc tcgtcgagcg tgaagccaag
     2521 ctctcgcgcg cggcgcacga atgtcaggcg acgaacgccg ccattaccgt aacagcgata
     2581 gcgccccgtc cgctccggtt cgggcaacag gccgatccgc tcatagtagc ggatcgtctc
     2641 gatgctgcac ccggtgcggc gtgacaattc gccaattcga atccctgcac tgcgcatcga
     2701 tggggttctc caggcaacgc ttgcgtctgt agttcctaca gagtgtagct tcgtccgata
     2761 cggggcaagg aggccataat gggcgagccg gtcgacattt cgcaggaccg aacaacgcag
     2821 ggcctgggtg ccgtggtgct tgccttgggc ggattgggtg cggcgtttgg tgtggcatca
     2881 tgctgcggcc tgcccttcct gctggcgaca gcgggtttgg ggacagcgtg gttgggcggg
     2941 gtcgcgttgc tggctgcacc gcatcggcaa tttctgctgg ttgcagcggc ggcgagcctc
     3001 gccg
//
LOCUS       DASSZH010000112         2405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_71196, whole genome shotgun sequence.
ACCESSION   DASSZH010000112 DASSZH010000000
VERSION     DASSZH010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71196"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(342..1241)
                     /locus_tag="VFN42_02275"
     CDS             complement(342..1241)
                     /locus_tag="VFN42_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995471.1"
                     /translation="MTQPLPDTSFAQRLYTTHYEALFKVYPILPPPLEDTPEGYARRD
                     AAVIEQIAQLVPASAIEAELAAKAVAGFEYWAHCLREAAPVPHDSPLHDRLTAQAARV
                     GRDARGYLASLQRVQTMRTKREATEAGAFRADVIEDNTRSTLQTVLAHPPKAQPVKAQ
                     QPALPEAGLPCPAAVPDTDAATPQPAPLPAEPAAPPVPLPHLTAVPRVVASPSPARRR
                     PRTHNDNGWPRPDWAEEAERYAIIYPERARLIRRLGRLPSPCTFGPPEKPLLRAIIAG
                     SSPALCALDGPEEADAGQRLRCA"
     gene            1526..>2405
                     /locus_tag="VFN42_02280"
     CDS             1526..>2405
                     /locus_tag="VFN42_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HET8995472.1"
                     /translation="MDEDFRRAALEYHRLPRPGKLTVQPTKRMASQRDLALAYSPGVA
                     AACEAIKADPDTARDYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKAVLFKKFA
                     GIDVFDIEVDAQDPDRFCDVVAALEPTFGAINLEDIKAPECFAIEATLRARMNIPVFH
                     DDQHGTAITVAAAVRNGLLLQGKDLSDARLVTSGAGAAALACVDLLVSMGLQPENVTL
                     TDVNGVVTTGRSGMLSNMARYARDTDAKTLAEVLPGTDIFLGLSAPGVLKPEWLPRMA
                     EKPLILALANPEPEITP"
BASE COUNT          353 a          801 c          864 g          387 t
ORIGIN      
        1 cgagctgttc gattgaccat ggcggatccc gccagcctgt cacggtcgtt cgagcaaccg
       61 gagaagggcg gcgggccgaa gcgattcggt ccggcatagc ggtgctggcc gaggcgcgat
      121 cgaatggcgt gtcttgcggc cgtctcgttg cacgctctct gggagtttgt ctcctggcat
      181 gagaatgatg gccggtggcc ggggcgattg cgtggctgcg actggccggt tgtcgccgat
      241 tgcagacgcg ggaattccgc cgaaccggtt ctgaacaagc ccgcgccggc gtggaagcgt
      301 ccgtgtagat ccgcctgggt gcgcggtgga cgcttccttg cctacgcgca tcgaagccgc
      361 tgccccgcat ccgcctcctc cgggccgtcc agtgcgcaca atgccgggct ggaaccggcg
      421 atgatcgccc gcagcagcgg cttctccggc gggccgaagg tgcatgggct gggcagccgg
      481 cccaggcggc ggatcagccg ggcacgttcg gggtagatga tggcatagcg ttccgcttcc
      541 tcggcccagt ccgggcgcgg ccagccgtta tcgttgtggg tccgcgggcg gcgccgggcg
      601 ggggagggag acgcgacgac ccttggcacc gcggtcagat gcggcagcgg gaccggcggg
      661 gcggccggtt cggccggcag cggtgcgggc tgcggagttg cggcatcggt gtccggcacg
      721 gcggcagggc agggcagtcc ggcttccggc agggccggct gctgcgcttt caccggctgc
      781 gctttcggcg gatgcgccag gaccgtctgg agcgtgctgc gtgtgttatc ctcaatgaca
      841 tcggcccgga aggcgccggc ctcggtcgcc tcgcgtttcg tgcgcatggt ctgcacgcgc
      901 tgcagcgacg cgagatagcc gcgtgcatcg cgtcccacgc gggcggcctg cgcggtgagc
      961 cggtcgtgca gcggcgagtc gtgcggcacc ggggcggctt cgcgcaggca gtgcgcccag
     1021 tattcgaagc cggcgaccgc cttggcggca agttccgctt cgatggcgct ggcggggaca
     1081 agctgggcga tctgctcgat caccgctgcg tcgcgccgag cgtagccttc cggcgtgtcc
     1141 tccagcggcg gcgggaggat cggatagacc ttgaacaggg cttcgtaatg cgtggtgtag
     1201 aggcgctggg cgaaggaggt gtcgggaagg ggctgggtca tgggacgtta ggataacgtc
     1261 agttagagta aatgtcaata ggaaattgcc gcgccagggc ctgcggcgcc gatggcgtgg
     1321 cgcccattct ggcgcagcct tgtactggcc aaacgaccgg ctgcttcgcc agccggcgcc
     1381 gtggcgaccg ggacaggccg atcaccgagg tagccgccgc actccgtgac ggggcttcga
     1441 catagcggcc gtcgcatcgc cgcatctggt cccgccccgc ctccacgctg tatgacgctc
     1501 tgaagccagt tgcgaggcca ctgccatgga cgaggacttc cgccgagcgg cactcgagta
     1561 tcaccgcctg ccacggcctg ggaaactcac ggttcagcct accaagcgca tggccagtca
     1621 gcgtgacctc gcgctcgcct actccccggg cgtcgccgcc gcctgcgagg cgatcaaggc
     1681 cgaccccgac accgcccgcg actataccgc ccgcggcaac ctcgtcgcgg tcatctccaa
     1741 cggcaccgcc gtgctcggcc ttggcaacat cggcgcgttg gccggcaagc cggtgatgga
     1801 aggcaaggcg gtgctgttca agaagttcgc cggcatcgac gtgttcgaca tcgaagtgga
     1861 cgcgcaggac cccgaccgtt tctgcgacgt ggtcgccgcg ctggaaccca ctttcggcgc
     1921 gatcaacctg gaggacatca aggcccccga atgcttcgcc atcgaagcga cgctgcgcgc
     1981 ccgcatgaac attccggtgt tccacgacga ccagcacggc acggccatca cggtcgccgc
     2041 cgcggtgcgc aacgggctgc tgctgcaggg caaggatctt tcggacgccc ggctggtcac
     2101 gtccggtgcc ggcgccgcgg cgctggcctg cgtggacctc ctggtttcca tggggctgca
     2161 gccggagaac gtgacgctga ccgacgtcaa cggtgtggtc accaccggcc ggtccggcat
     2221 gctgtccaac atggcacgct acgcccgcga caccgatgcg aagacgctgg ccgaggtgct
     2281 gccgggcacc gacatcttcc tcggcctctc cgcccccggc gtgctgaagc cggaatggct
     2341 gccgcgcatg gcggaaaagc cgctgattct ggcgctggcc aaccccgagc cggagatcac
     2401 ccccg
//
LOCUS       DASSZH010000113         5432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1264369, whole genome shotgun sequence.
ACCESSION   DASSZH010000113 DASSZH010000000
VERSION     DASSZH010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5432
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264369"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            350..964
                     /gene="rpsD"
                     /locus_tag="VFN42_02285"
     CDS             350..964
                     /gene="rpsD"
                     /locus_tag="VFN42_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011253300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET8995473.1"
                     /translation="MTKRAESKYKINRRLGINLWGRSKSPVNKREYGPGQHGQRRKKP
                     TDFGTQLMAKQKLKGYYGNISEKQFRKYYEEAVRRKGDTSENLIELLERRLDAVCYRL
                     KFAVTPFAARQFVSHGHILVNGKRVNIPSYLVKDNDTIEVKEKSKQLAMVLDAAQSTE
                     RDVPEYLEVDHRAMKGRFARAPKLSDVPYPVQMEPNLVVEFYSR"
     gene            1139..1897
                     /locus_tag="VFN42_02290"
     CDS             1139..1897
                     /locus_tag="VFN42_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995474.1"
                     /translation="MLFGLFASGSAVARNAAGTELSPGQLTQRARQASPEELAQAWLG
                     ADKMVRLAAVARGGASVVMQSPGREQTMDASNADALARTFQERRSIFEAEIKRRGFAA
                     FAGSYTIHVADSCVAANAPAWLLNRSPQAKGNASTKFATQVRIRQQGFHVTLIDHAED
                     VRSGEALDIEMDGAAAESSFVLEDRFSGDILYAGRMTGRDITIRPMAELIQGTYSSYP
                     GWIARPDANALRACTITLTAAKPPASRQGSRHRQ"
     gene            complement(1891..3075)
                     /locus_tag="VFN42_02295"
     CDS             complement(1891..3075)
                     /locus_tag="VFN42_02295"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995475.1"
                     /translation="MNVVSRIARAAPDPVSRARELGPAIAAAADEVERTQCIAEPLLS
                     QIHAARLCRMLLPRSVDGDQVEPWVYLRAIEELSRHDGSVGWNVFVANSSALIAPFLP
                     PATMQAIFADPRAVVSWGPPNASKAHAVPGGYRVSGEWHFASGCRQATWMGAHCQVVE
                     PDGSLRLNAAGRPLIRSLLFPAAHVTHIEDWNPIGLRGTASEGYTLTDLFVPEDFSGT
                     REEPEQRREPGRLYAFTMQGLYAVGVAGVAFGIARAMLDAFIALASHKAPRGLARLGD
                     SATVQAGVARQEAALGAGRAYLVETLQNIWDTADDGLGVIDIPARARVRLACAQAIQA
                     AIGVADFTYKAAGVDAIFPGTPFERRFRDMHTLSQQIQSRDSHFEAVGAILLGHTPAV
                     FY"
     gene            complement(3189..4421)
                     /locus_tag="VFN42_02300"
     CDS             complement(3189..4421)
                     /locus_tag="VFN42_02300"
                     /inference="COORDINATES: protein motif:HMM:NF006769.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HET8995476.1"
                     /translation="MSPTINADRLWQSLMELARIGATPEGGNCRLALSAEDTQARALF
                     LRWCQPLGLHYEQDAIGNMFLRRDGRDPTAGAVAFGSHLDTVPTGGRFDGPFGVLAGL
                     EVLRALHEAGVTTDKPLELVNWTNEEGSRFRPAMMGSRVHAAEMTLETALATTDDQGI
                     SVAQALKESGQAGPLPPSPRGWTCWLEGHIEQGPVLEATSTDIGVVTGTMHARYFQLT
                     VTGEPSHVGPTTMDRRRDSLAAAAEIILAVERIALDAEPLGRASATWIENYPNARGNV
                     ANVTRLHCDVRHEDAQRAVAMEAALRAAIDAIALRRQVQVAIDPYATFGPIVFDQTLC
                     AVLRGKAEQRQLSTRDMTAAAGHDSVLIAPLCPSAMLFVPSVGGITHNPKEYSTPEQV
                     ARGAQVLLDAVLELAGSS"
     gene            4528..4788
                     /locus_tag="VFN42_02305"
     CDS             4528..4788
                     /locus_tag="VFN42_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995477.1"
                     /translation="MDTQDSGERDYAGEQTLQLPVDGYPISEDAVAHWFQQTYRREPT
                     DLELGAIISAMAERETTPPHVGPHAEPEGWKVGPSAPPADRR"
     gene            complement(4806..5411)
                     /locus_tag="VFN42_02310"
     CDS             complement(4806..5411)
                     /locus_tag="VFN42_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995478.1"
                     /translation="MLLLRDPWLISQTLMQLLYLLPAAFLVWRGFYARGGTLALLVPV
                     LIVTAGQLAGGLAWLAVSGEDAPELIASAPVSPAGILRAKTEAVLGGIALVFAPFVLV
                     LAAGAPFPALVAIGGIAISAGSAIAIQFWFRTQARRSLFRRRQTSSRIATFAEALSSI
                     GWAGTGALAAAGTWLASIPGLLVIAVLVGAWAISPGRPPAA"
BASE COUNT          924 a         1798 c         1889 g          821 t
ORIGIN      
        1 ggcacggcgg cgacgggggc gccgggcagc atcatcagcc agccccaggg ctgaccgttg
       61 acgataagat ccaagccgat gccaagcatc atgccgccga tcaggctgcc catgacgccc
      121 gcggacacgc caagccacag gatttcggaa cgggtaatca tggatgggct agctaccggc
      181 ggggggaatg tttgacaagc gggctgccga acgcctatag gccgccgctt cacggccggg
      241 aacgtggacg gaccgccagg gaatcctggt ctggcccgcg cccgtgcgtt ggcatgccgc
      301 cttcgcgcct accggtgcaa caacatggac gaaaagggca acgcgccaca tgaccaagcg
      361 cgccgagagt aaatacaaga ttaaccgccg gttgggcatc aacctgtggg gccggtcaaa
      421 gtcccccgtc aacaagcgtg aatacggtcc gggccagcac ggccagcggc gcaagaagcc
      481 gaccgatttc ggcacgcagt tgatggcgaa gcagaagctg aagggctact acggcaacat
      541 cagcgaaaag cagttccgca agtattatga ggaagcggtc cgccggaagg gcgacacgtc
      601 agagaacctg atcgagctgc tggagcgccg gctggacgcg gtgtgctacc gcctgaagtt
      661 cgccgtgacc ccgttcgcgg cacggcagtt cgtctcgcac gggcacatcc tggtcaacgg
      721 caagcgggtg aacattccct cctatctcgt gaaggacaac gacacgatcg aggtgaagga
      781 gaagtcgaag caactcgcca tggtgctgga cgcggcgcag agcaccgagc gcgatgtgcc
      841 ggaatacctg gaagtggatc accgggcgat gaagggccgc ttcgcgcgcg cgccgaaact
      901 gtccgatgtg ccgtatccgg tacagatgga accgaacctg gtggtcgagt tctactcgcg
      961 gtaacggctc cgcggtggcg ggaccagccc gccgccgcgc ggcaaatcgc tctggcggca
     1021 cgtttttgat ctgagtcaat ggcgcgtgcg tcaggttggc gtatggcgcg gttcgacacg
     1081 aaccgcgggg cccgccatgc ccagtgacct gcgtcgcctt ccatttgctg cggctctggt
     1141 gctgttcggc ctgttcgcat ccgggagcgc ggtggcgcgg aacgcggccg ggacagagct
     1201 ttcccccggg cagttgacgc aacgggcacg ccaagcctcg cctgaggagt tggcgcaggc
     1261 ctggttgggc gcggacaaga tggtccggct cgccgccgtc gcgcggggtg gcgcatcggt
     1321 ggtcatgcag tctcccggcc gggaacagac catggatgca tcgaatgcgg atgccctcgc
     1381 gaggacattc caggagaggc ggagcatttt cgaggcggaa atcaagcgcc gcggctttgc
     1441 cgccttcgcc ggcagctaca cgatccatgt tgcggattcc tgcgtcgcgg cgaacgcgcc
     1501 cgcctggctg ctgaaccgct cgccccaggc gaaaggcaat gcgtcgacga agttcgcaac
     1561 ccaggtgcgg atccgtcagc aggggtttca cgtcaccctg atcgatcatg ccgaggatgt
     1621 caggtccggc gaggcgctcg atatcgagat ggatggcgcc gcggccgagt ccagctttgt
     1681 gctggaggac cggttcagtg gcgacattct ctatgccgga cgcatgacag ggcgcgacat
     1741 caccattcgt cccatggccg aactgatcca gggtacctat tcgtcctatc ccggatggat
     1801 tgcccgcccg gatgcaaacg ctttgcgcgc ctgcacgata accttgaccg ccgcgaagcc
     1861 gccggcgtca cgtcagggca gcaggcaccg tcagtagaac accgcaggcg tgtgcccgag
     1921 caggatggcg ccgacagcct cgaagtggga atcgcgcgac tggatctgct gcgacagcgt
     1981 gtgcatgtcg cggaaccggc gctcgaatgg cgtgcccggg aagatcgcgt ccacgccggc
     2041 cgccttgtag gtgaaatccg ccacgccgat cgccgcctgg atggcctgcg cgcaggccag
     2101 ccgcacgcgc gcccgcgcgg ggatgtcgat cacgccgaga ccgtcatccg ccgtgtccca
     2161 gatgttctgc aaggtttcca ccagataggc gcgtccggcg cccagcgcgg cttcctgtcg
     2221 cgcgacgccg gcctgcaccg tggcgctgtc gcccagccgg gcaaggccgc gcggcgcctt
     2281 gtgcgaggcg agtgcgatga acgcgtccag catggcgcgc gcgatgccga aggcgacgcc
     2341 cgccacgccc accgcgtaca ggccctgcat ggtgaaggcg tacagccggc cgggttcgcg
     2401 gcgctgttcc ggttcctcgc gggtaccgct gaaatcttcc ggcacgaaca ggtcggtcag
     2461 cgtgtagcct tccgatgcgg tgccgcgcag gccgatcggg ttccagtcct cgatatgcgt
     2521 cacgtgcgcc gccgggaaca gcaatgagcg gatcagcggc cgtcctgccg cgttcagccg
     2581 cagcgagccg tccggttcga ccacctggca gtgcgcgccc atccaggtgg cctggcggca
     2641 tccgctggcg aagtgccatt cgccggacac gcggtagccg ccgggcaccg catgcgcctt
     2701 gctggcgttg ggcggacccc aggacaccac cgcacgcggg tcggcgaaga tcgcctgcat
     2761 ggtggcgggt ggcaggaacg gcgcgatcag cgcggagctg ttggcaacga acacgttcca
     2821 gccgaccgaa ccgtcgtgcc ggctgagctc ctcgatcgcg cgcagataga cccagggttc
     2881 cacctggtcg ccatccaccg agcgtggcag gagcatgcgg cacagacgcg cggcatgaat
     2941 ctgcgacagc agcggttcgg caatgcattg ggtgcgctcg acctcatccg cggcggcggc
     3001 aatggcgggg ccaagttcgc gggcacggga taccggatcg ggcgcggcgc gggcaatcct
     3061 gctgacgacg ttcatgcgct gcctctggac gttttgaggc gattaccgga gccgcgggcg
     3121 gcgctgtcaa ctccgccggt ctgcaagctc gggcgttacc gcaccgccca tctccggctg
     3181 cggttccgct aggacgaacc cgccagctcc agcacggcat ccagcagaac ctgcgcaccg
     3241 cgcgccacct gctcgggtgt cgagtattcc ttgggattgt gcgtgatccc gccgacgctc
     3301 ggcacgaaca gcatggcact ggggcacagc ggcgcgatca gcacggaatc gtgcccggct
     3361 gccgcggtca tgtcgcgcgt gctcagctgc cgctgctctg ccttgccgcg cagcaccgcg
     3421 cacagggtct ggtcgaagac gatcgggcca aacgtggcat agggatcgat cgccacctgt
     3481 acctgccgcc gtagcgcgat ggcgtcgatc gcggcgcgca atgccgcctc catcgccacg
     3541 gcgcgttgcg cgtcctcgtg ccgcacgtcg cagtgcagcc gggtcacatt ggcgacgttg
     3601 ccgcgtgcat tcggatagtt ctcgatccag gtcgccgatg cgcggcccag cggttccgcg
     3661 tcgagcgcga tgcgttcgac cgcaaggatg atctctgctg ccgctgccag gctgtcgcgc
     3721 cgccggtcca tcgtggtcgg ccccacatgg ctcggctcgc cggtgaccgt gagctggaaa
     3781 tagcgcgcgt gcatggtgcc ggtgacgacg ccgatatcgg tgctggttgc ctccagcacg
     3841 gggccctgct cgatatgccc ctccagccag cacgtccagc cgcgcgggga aggcggcagc
     3901 ggaccggcct gcccgctctc cttcagtgcc tgcgcgacgc tgatcccctg atcgtcggtg
     3961 gttgccagcg ccgtttccag cgtcatctcc gcggcgtgta cccggctgcc catcatggcg
     4021 gggcggaacc ggctgccttc ctcgttggtc cagttcacca actccagcgg cttgtccgtc
     4081 gtcacgccgg cctcatgcag tgcgcgcagc acttccagcc cggccagcac gccgaacggt
     4141 ccgtcgaagc gcccgcccgt gggcaccgta tccaggtggc tgccgaaggc gaccgcgccg
     4201 gcggtgggat cccggccgtc tcggcgcagg aacatgttgc cgatggcgtc ctgctcgtag
     4261 tgcaagccga gcggctggca ccagcgcagg aacagggcgc gggcctgggt gtcttccgcg
     4321 gacagagcga ggcggcaatt gccgccttcc ggcgtcgcgc cgatccgcgc gagttccatc
     4381 agcgattgcc agaggcggtc ggcattgatc gtcggggaca tggggcctcc ggtgaatggg
     4441 cgggggaact aggccaggaa gcgggcggtc tcgcaaccgg cacgctccgc gtctgttgag
     4501 agacgaagta ctgcatggag catcggcatg gatacgcaag acagtggtga gcgggactac
     4561 gcgggcgagc agacattgca gttgccggtc gacggctatc cgatttccga ggatgcggtg
     4621 gcgcattggt tccagcagac ctatcggcgc gagccgacgg acctggaact tggtgcgatc
     4681 atcagtgcga tggcggagcg ggaaaccacg ccgccgcatg tcggccccca tgcggagccg
     4741 gagggctgga aagtagggcc gtccgcgccg ccggcggacc ggcgttagcg cagcaggacg
     4801 gcgggtcagg cggctggcgg tcggcccggg ctgatcgccc aggcgccgac cagcaccgcg
     4861 atgacgagca ggccggggat gctggcgagc caggtgccgg ctgcggcgag tgcccccgtc
     4921 cccgcccaac cgatcgacga cagcgcctcg gcaaaagtcg cgatgcgcga cgacgtctgg
     4981 cggcggcgga acaggctgcg ccgggcctgc gtgcggaacc agaactggat tgcgatggcg
     5041 gagccggccg agatggcgat tccgccgatc gcgaccagcg ccgggaacgg cgccccggct
     5101 gcgagcacaa ggacgaaagg cgcgaacacg agcgcgatgc cgcccagcac cgcttcggtc
     5161 ttcgcgcgga gaatgccggc cggcgagacg ggtgccgagg cgatcagttc gggcgcgtcc
     5221 tctccggata cggcaagcca ggccaggccc cccgccagct gtccggcggt gacgatcagc
     5281 accggcacca gcaatgccag cgtgccgccc cgggcgtaga acccgcgcca caccaggaac
     5341 gccgcaggca acaggtaaag cagttgcatc agcgtctgcg agatgagcca gggatcgcgc
     5401 agcagcagca cccactcctt gcggcgcaag gt
//
LOCUS       DASSZH010000114         2832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_122484, whole genome shotgun sequence.
ACCESSION   DASSZH010000114 DASSZH010000000
VERSION     DASSZH010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122484"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..492
                     /locus_tag="VFN42_02315"
     CDS             208..492
                     /locus_tag="VFN42_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HET8995479.1"
                     /translation="MPELHTRAERLESALRTAFAPERLEVVDDSARHAGHAGAQPGGQ
                     THYSVLVVSEAFRGMNRVARSRAVHAALEQEFAGGMHALALTLRTPEEAG"
     gene            complement(532..1413)
                     /locus_tag="VFN42_02320"
     CDS             complement(532..1413)
                     /locus_tag="VFN42_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HET8995480.1"
                     /translation="MLASLRLRRDPTQPVPNTMLICQLTDLHVRPVGKPSNRVSETNM
                     FTQRAFEAVAAFAPRPDVVVISGDLTECGLAEEYANLSSLIARHLPMPVFVIPGNHDR
                     RENLRAALAHLPGVTVDPDYVQYTVDDYPVRLVLLDTLVPGAGHGELRPGQLQWLDRT
                     LSAEPHKPTMIAMHHPPFACGIAHMDRIMLRNTADFAGVIARHQQVERIICGHHHRPI
                     VARVGPAIASICPSVAHQVEMSLDPQGAANFVFEPPAFQLHRWTPAEGIVSHTAYVER
                     FPGPFPFITDPDYPGKG"
     gene            complement(1496..2569)
                     /gene="yejB"
                     /locus_tag="VFN42_02325"
     CDS             complement(1496..2569)
                     /gene="yejB"
                     /locus_tag="VFN42_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HET8995481.1"
                     /translation="MTAYLIRRLLLVIPTLFGIIVINFVVIQFAPGGPVEQAISQLRG
                     KGALSSQLGAAASDVGSGNAGLYRGASGLDPHVVAEIRKMFGFDKPPLTRFLDMVGGY
                     LRFDFGRSFFQGKSVIRLIAERLPVSISLGLWSTLLVYTISIPLGIAKAVRDGTHFDV
                     ATSAAVLVGYAVPSFLFAILLVVVFAGGSYLDWFPLRGLTSMGSQHWPWPERVGDYFW
                     HLVLPVTAIVAGGFASLTMLTKNCFLEEIRKQYTVTAMAKGASERRVLYGHVFRNAML
                     LIVAGFPQAFIGILFTSALLVEIVFSLNGLGLLGFQAAIQRDYPVMFGTLYIFTLLGL
                     LMQIVGDMLYTMIDPRIDFARRD"
BASE COUNT          522 a          928 c          973 g          409 t
ORIGIN      
        1 cagtagtcgt tcaggcggct ccggtccttc ggtgcgcggt actcgccctg cgcgccgcaa
       61 ccgggcatgt cgcaggtccg ccccggagcg gccgggtcgg gcgcgtaggc cctgggtcgc
      121 gtgggacgtc gggtcatgcc agcctatgtg ccgtcgcgcc cgtggaatgg caactggcgg
      181 gttgatgaca agaggagatt ggcggctatg ccggaattgc acacacgcgc ggagcggctg
      241 gaatcggcgt tgcggacggc cttcgcccca gagcgactgg aagtggtgga cgacagtgcc
      301 cgccacgccg gccacgccgg ggcacagccg ggcgggcaga cgcattacag cgtgctggtg
      361 gtctcggagg cattccgcgg catgaaccgg gtggcgcggt cacgcgcggt gcacgcggcg
      421 ctggagcagg agttcgccgg cggcatgcac gcgctggcac tgacgctgcg cacgccggag
      481 gaggcaggct gaaccgagct cacgcgccgc tgcgcccggc cgcggcgacg ctcagccctt
      541 gcccgggtaa tccggatccg tgatgaatgg aaacgggccg gggaaccgtt ccacatacgc
      601 ggtatgcgac acgatcccct cagccggcgt ccagcgatgc aactgaaacg ccggcggctc
      661 gaacacgaaa ttggccgcgc cctgcggatc cagcgacatc tccacctgat gcgccaccga
      721 gggacagatc gaggcgatcg ccggcccgac ccgcgcgacg atcggccggt gatggtggcc
      781 gcagatgatc cgctccacct gctggtgccg cgcgatcact ccggcaaaat ccgccgtgtt
      841 gcgcagcatg atccggtcca tatgcgcgat cccgcaggca aacggcggat gatgcatggc
      901 gatcatcgtc ggcttgtgcg gttcggcgct cagcgtccgg tccagccact ggagctggcc
      961 gggacgcaat tcgccgtgac cggcaccggg caccagcgtg tccagcagca cgagccgcac
     1021 cgggtaatcg tccaccgtgt attgcacgta gtccggatcg accgtcacgc ccggcagatg
     1081 cgccagggcc gcgcgcaggt tctcccgccg gtcgtggttc ccgggaatga cgaagaccgg
     1141 catcggcagg tggcgtgcga tcaacgatga caggttggcg tattcctccg ccagcccgca
     1201 ctccgtcagg tcgccggaaa tgaccaccac atccgggcgc ggcgcgaagg cggcgaccgc
     1261 ctcgaaggcg cgctgggtga acatgttggt ttccgacacc cggttggagg gcttgcccac
     1321 ggggcggaca tgcaggtcgg tgagttggca gatcagcatc gtgttcggca ccggttgcgt
     1381 ggggtcgcgc cgcaggcgca aggatgccag cattacgcgg ggcacccccc tggcttcaag
     1441 cgacaatccg aggacgggcg ctggcgccgg atcgtccagg cggcatgccg ccaggctaat
     1501 cccgccgcgc gaaatcgatg cgcggatcga tcatcgtgta cagcatgtcg ccgacgatct
     1561 gcatcagcag gccgagcagg gtgaaaatgt acagcgtgcc gaacatcacc ggatagtcgc
     1621 gctggatcgc cgcctggaag cccagcaggc ccagcccatt cagcgagaac acgatctcca
     1681 ccagcagggc cgaggtgaac aggatgccga tgaacgcctg cggaaagccg gcgacgatca
     1741 gcagcatggc gttgcggaac acgtggccgt acagcacgcg ccgctcgctg gcgcccttgg
     1801 ccatggcggt gacggtgtac tgcttgcgga tctcctccag gaagcagttc ttcgtcagca
     1861 tggtcaggct ggcaaacccg ccggcgacga tcgcggtgac aggcagcacg agatgccaga
     1921 aataatcgcc gacacgctcc ggccagggcc agtgctgcga tcccatgctg gtcaggccgc
     1981 gcaacggaaa ccagtccagg taactccccc cggcgaacac caccaccagc agaatcgcga
     2041 acaaaaagct cggcaccgcg tagccgacca gcaccgcggc actggtcgcc acgtcgaaat
     2101 gcgtgccgtc gcgcaccgcc ttggcaatgc cgagcggtat ggagatggta tagaccagca
     2161 gcgtcgacca taagcccagc gagatcgaca ccggcaatcg ctccgcgatc agccggatca
     2221 ccgacttgcc ctggaaaaag ctgcggccga agtcgaagcg cagatagcct ccgaccatgt
     2281 cgaggaagcg ggtcagcggc ggcttgtcga agccgaacat cttgcggatt tccgcgacga
     2341 cgtgcggatc gagcccgctg gcgccgcgat acagcccggc attgccgctg ccgacatccg
     2401 acgccgcggc gccaagttgc gacgacagcg cccccttgcc gcgcagttgg gaaatcgcct
     2461 gctcgaccgg gccgccgggc gcgaattgta tcaccacgaa gttgatgacg atgatgccga
     2521 acagcgtcgg gatcaccagc agcagccggc ggatcagata ggcggtcatg ggcgagttga
     2581 tggagcagca gggtggaaag gaaactcccc ctccccttgc gggagggggg cggggggagg
     2641 gggtgaatgt agttgcccgg ggaacccatg caggccccct cccccgcccc cctcccacaa
     2701 ggggaggggg agttcttgag gatccttgcc atcaatgcgc ccgcgcggcg tcggtggcct
     2761 tggctttggc cggatcgacc caccagcagt cgaagttgac gccctcgcgg atcgggatgt
     2821 ccggacggcc ga
//
LOCUS       DASSZH010000115         4035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_156463, whole genome shotgun sequence.
ACCESSION   DASSZH010000115 DASSZH010000000
VERSION     DASSZH010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156463"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..1363
                     /locus_tag="VFN42_02330"
     CDS             23..1363
                     /locus_tag="VFN42_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HET8995482.1"
                     /translation="MQHPRSPSHHRGRTPFHTLAGRIALHRAPIPGRPGLLARLGPGL
                     ITGASDDDPSGIATYAQVGAQFGYAMCWVMLFSYPLMAAIQEISARIGRVTGRGIAGN
                     IRFHYSPWLLRGIVGLLLIANTINLGADLGAMGDALALLIAGPAQLYVVGFAGFCAVL
                     EVFSSYERYVRVLKWTSVSLFAYVAVAFAVHVPWHLMLLHTLVPNFVWRQDYIVAIVA
                     VLGTTITPYCFFWQSSQEAEDERLDPHGHTLLDAPDDAPAQLGRMRFDTYVGMGYSNL
                     ISLFIMVATAATLNAHGITNITTSAQAVEALRPISGPFAALVFAAGIIGIGLLAVPVL
                     AGSGAYALGEALDWPTGLGRRPLDAKAFYGTIVVSTLLGVAINFTQVDPIKALFWTAV
                     LNGVISVPLMVVMMVMARDPKVMGRFVLPRPLWAMGWICTLAMLAAVGIMFITW"
     gene            1455..2729
                     /gene="eno"
                     /locus_tag="VFN42_02335"
     CDS             1455..2729
                     /gene="eno"
                     /locus_tag="VFN42_02335"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET8995483.1"
                     /translation="MAAIADIIAREILDSRGNPTVEVDVVLDSGAMGRAAVPSGASTG
                     AHEAVELRDNDASRYGGKGVQQAVANVQGEILEEIAGLEATEQVRIDNALIDLDGTAN
                     KARLGANAILAVSLAVAKAAAADLDLPLYRYVGGVYARTLPVPMMNIVNGGKHADNPI
                     DIQEFMIQPLGAGTLADAVRMGSEIFHALRKGLPDAGHSTNVGDEGGFAPNLKSADEA
                     LGFVAKACEKAGYRLGEDVAFALDPASSEFYHDGKYVLAGEGKTLDAAGIVQYYEALV
                     SRYPIISIEDGCAEDDWEGWQHLTATLGSRIQIVGDDLFVTNPERVRRGLEEASANAV
                     LIKVNQIGTLTETLETVELAHRAGWRCVMSHRSGETEDATIADLAVATNCGQIKTGSL
                     ARSDRTAKYNQLIRIEELLGPSARFAGRTILR"
     gene            2877..3179
                     /locus_tag="VFN42_02340"
     CDS             2877..3179
                     /locus_tag="VFN42_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006115173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HET8995484.1"
                     /translation="MAKASAAPLVFCSITAYFGWNATQGAHGLVTFAQRKTQLAQAET
                     DLAAAKAERDAWQTRVSGLRASDLNLDTLDERARAMLNLAEPNEILVQYPTKDRLF"
     gene            complement(3237..3950)
                     /locus_tag="VFN42_02345"
     CDS             complement(3237..3950)
                     /locus_tag="VFN42_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8995485.1"
                     /translation="MPGPLDGSVALVTGASRGLGAAVAVELGRLGAHVVLTARTQGGL
                     EETDDAIRSAGSSATLLPLDLKEGEPVDAIGPSLYQRFGRLDVLVHAAGVLGKLTPVS
                     HIMPNDWAEAVAVNLDAAMRLIRTCEPLLRQAPAGRAVFVTDERAGAPLAYWATYGAT
                     KAAMEHLVRTWAQELQITRARANLFDPGPFRSRLRNHAFPGIDPATLALPEDVAPGLV
                     PLCLPEEQRSGALVRGGNG"
BASE COUNT          639 a         1455 c         1330 g          611 t
ORIGIN      
        1 aattcatgag cacacgggtc gtatgcaaca tcctcgcagc ccgagccacc accgcggccg
       61 aaccccgttc cacaccctgg ccggccgcat cgcgctgcac cgcgcgccga ttcccggccg
      121 ccccggcctg ctggcgcggc tcggccccgg gctgatcacc ggcgcgtcgg acgacgatcc
      181 cagcggcatc gcgacctacg cgcaggtcgg tgcgcagttc ggctacgcca tgtgctgggt
      241 catgctgttc agctacccgc tgatggccgc catccaggag atcagcgccc gcatcggccg
      301 cgtcaccggc cgcggcatcg ccggcaacat ccgcttccac tattcgccct ggctgctgcg
      361 cggcatcgtc ggcctgctgc tgatcgccaa caccatcaac ctcggtgccg acctcggcgc
      421 catgggcgac gcgctggcgc tgctgatcgc cggcccggcg cagctctacg tggtcggctt
      481 cgccggcttc tgcgccgtgc tggaggtatt ctccagctac gaacgctatg tccgcgtgct
      541 gaaatggacc agcgtgtcgc tgttcgccta tgtcgccgtc gccttcgcgg tgcacgtgcc
      601 gtggcacctg atgctgctcc acaccctggt gccaaacttt gtctggcgcc aagactacat
      661 cgtggcgatc gtcgcggtcc tgggcaccac catcacgccg tactgcttct tctggcagtc
      721 gtcccaggaa gccgaggacg aacgccttga cccgcacggg cacaccctgc tggacgcgcc
      781 cgacgacgct cccgcccagc tcggccgcat gcggttcgat acctatgtcg gtatgggcta
      841 ttccaacctg atcagcctgt tcatcatggt cgccaccgcg gcgacgctga acgcacacgg
      901 catcaccaac atcacgacat ccgcccaggc ggtggaggcg ttgcggccca tctccggccc
      961 cttcgccgcg ctggtgttcg ccgccggcat catcggcatc ggcctgctgg cggtgccggt
     1021 gctggccggc tccggcgcct atgcgctggg cgaggcgctg gactggccca ccggcctggg
     1081 ccgccgcccg ctcgacgcca aggcgttcta cggcaccatc gtggtgtcca ccctgctcgg
     1141 cgtcgccatc aacttcaccc aggtcgatcc gatcaaggcg ctgttctgga ccgcggtgct
     1201 gaacggcgtg atttccgtgc cgctgatggt ggtgatgatg gtgatggcgc gcgaccccaa
     1261 ggtgatgggc cgcttcgtgc tgccgcggcc gctctgggcc atgggctgga tctgcacgct
     1321 ggcgatgctg gcggcggtcg gcatcatgtt catcacctgg taacccgccg gcacgacact
     1381 gccccggact ggcacgggct gccgcttggt ggtacccacc cgcccgcgtc acagacagtc
     1441 cggagcaccc gagcatggcc gcgatcgccg acatcatcgc ccgcgaaatc ctcgacagcc
     1501 gcggcaatcc caccgtcgaa gtggatgtgg tgctggacag cggcgccatg ggccgcgccg
     1561 cggtgccctc cggcgcctcc accggcgcgc atgaggcggt ggaactgcgc gacaacgacg
     1621 caagccgcta cggcggcaag ggcgtgcagc aggcggtcgc caacgtgcag ggcgagatcc
     1681 tggaggagat cgccggcctg gaagccaccg agcaggtgcg catcgacaac gcgctgatcg
     1741 acctggacgg cacggcgaac aaggcgcggc tgggcgccaa cgccatcctg gcggtatcgc
     1801 tggcggtggc gaaggcggcg gcggcggatc ttgacctgcc gctgtaccgc tacgtcggcg
     1861 gcgtctatgc gcgcacgctg ccggtgccga tgatgaacat cgtcaacggc ggcaagcatg
     1921 ccgacaaccc gatcgacatc caggaattca tgatccagcc gctcggcgcc ggcacgctgg
     1981 ccgacgccgt gcgcatggga tcggagatct tccacgccct gcgcaagggc ctgcctgacg
     2041 ccggccacag caccaatgtc ggcgacgagg gcggcttcgc ccccaacctg aaatccgccg
     2101 acgaggcgct gggcttcgtg gccaaggcct gcgagaaagc cggctaccgc ctgggcgagg
     2161 acgtcgcctt cgcgctggat cccgccagca gcgaattcta ccacgacggc aaatacgtgc
     2221 tggccggcga gggcaagacg ctcgatgctg ccggaatcgt gcagtactac gaggcgctgg
     2281 tctcacgcta tccgatcatc tcgatcgagg atggctgcgc cgaggacgac tgggaaggct
     2341 ggcagcacct gaccgccacg ctgggcagcc gcatccagat cgtcggcgac gacctgttcg
     2401 tcaccaatcc ggagcgcgtg cgccgcggac tggaggaagc cagcgccaac gcggtgctga
     2461 tcaaggtgaa ccagatcggc acgctgaccg agacgttgga aacggtggaa ctcgcccacc
     2521 gcgccggctg gcgctgcgtg atgagccacc gttccggcga gacggaggat gccaccattg
     2581 ccgacctggc ggtggcgacc aattgcgggc agatcaagac cggcagcctg gcgcggagcg
     2641 accgcaccgc aaaatacaac cagctgatcc gcatcgagga gctgctcggc cccagcgccc
     2701 gcttcgccgg ccgcaccatc ctgcgctgac ccgggccgct cgcggtcacg tgccgacaag
     2761 caacactgcc gcgtaatccc gccaatccgg ttcacccgcc ggtgcgctta cgctatggtg
     2821 cgccgacgtg attcggggcg ttgcaggcga tgatggtggg gcgcggactg aagcggttgg
     2881 caaaagcctc ggcggcgccg ttggtatttt gcagcatcac cgcctatttt ggctggaatg
     2941 cgacgcaggg cgcgcacggc ttggttactt tcgcgcaacg gaagacccag ctggcgcagg
     3001 ccgaaaccga cctcgccgcg gccaaggccg aacgcgacgc ctggcagacc cgggtatccg
     3061 gcctgcgggc cagcgatctc aacctggaca cgctggatga acgcgcccgc gcgatgctga
     3121 acctggcgga gccgaacgag atcctggtgc agtatccgac caaggaccgg ttgttctaac
     3181 cccggatcgg ccgcaccgcc gaagcagcag agcacggccg gatcgcgcgg cgccgttcat
     3241 ccattgccgc cccgcaccag cgcccccgac cgctgctcct ccggcaggca cagcggcacc
     3301 aggcccggcg ccacgtcctc cggcaaggcc agcgtcgccg ggtcgatccc agggaaggcg
     3361 tggttgcgca accggctgcg gaacggcccc ggatcgaaca ggttggcgcg cgcccgggtg
     3421 atctgcagtt cctgcgccca ggtgcgcacc aggtgctcca tcgccgcctt ggtcgcgccg
     3481 taggttgccc aatacgccag cggcgcaccg gcccgttcgt cggtgacgaa caccgcgcgg
     3541 ccagccggcg cctggcgcag cagcggctca caggtgcgga tcagccgcat cgcggcgtcc
     3601 aggttcaccg ccaccgcctc ggcccagtcg ttcggcatga tgtgcgacac cggagtcagc
     3661 ttgcccagca cgcctgccgc atgcactagg acgtccagcc ggccgaaacg ctgatagagg
     3721 ctggggccga tcgcatcgac cggctcgccc tccttgaggt ccagcggcag cagtgtggcc
     3781 gaactgccgg ctgaacgaat ggcgtcgtcg gtctcctcca gcccgccctg ggtgcgcgcg
     3841 gtcagcacca cgtgcgcgcc gagcctgccg agttccaccg caacggcggc gcccagcccc
     3901 cggctggcgc cggtcaccag cgccacgctg ccgtccagcg gtcccggcat cagtggtgtt
     3961 ccgccagcag gctcagctgc cgttcggcgt tgtccgcctg gtcgggcagc ggcaccgggt
     4021 agtcgccggt gaagc
//
LOCUS       DASSZH010000116         3167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_940674, whole genome shotgun sequence.
ACCESSION   DASSZH010000116 DASSZH010000000
VERSION     DASSZH010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940674"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /gene="bktB"
                     /locus_tag="VFN42_02350"
     CDS             <1..932
                     /gene="bktB"
                     /locus_tag="VFN42_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketothiolase BktB"
                     /protein_id="HET8995486.1"
                     /translation="TLNRLCGSGLQAVVSAAQAITLGDADIAVAGGAESMSRAPYMAS
                     GMRWGARMGDSAMIDMMLAALHDPFDHIHMGETAENLAESHQITRAQQDALAEQSHQR
                     AARAIKEGRFKSQILPVTLKSRKGETVFDTDEHVRAEASAADLGKLRPVFRKDGTVTA
                     GNASGINDGAAAVVLMDADTASKRGAKPLGRLVAYAHAGVDPKIMGIGPVPATRKALQ
                     RAGLTLADMDVIESNEAFAAQACAVAKDLGFDPEKTNPNGSGISLGHPVGATGTILTV
                     KALYELARIGGRYALVTMCIGGGQGIAAIFERV"
     gene            1195..2298
                     /locus_tag="VFN42_02355"
     CDS             1195..2298
                     /locus_tag="VFN42_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HET8995487.1"
                     /translation="MTEALSPRDVARLLQEPSAAMRAEVAAKVARQIDDPRLSPQELS
                     LAQEIARIMARDVALAVRRALADSLRRARRLPREVALRLAEDIDAVALPILAESPVLT
                     DDDLLRILHHASPRKQEAIAARPNLAAPVCDAVAESAEEPAVAALMRNPTASLTLRSL
                     HRAIDRFAASQAVQESIVHRASLPMDIAERLVTVISDRMRDYLVSHHALPSDSAADII
                     LRGRERAMLQLSQGAGQDELLRLLRQMHWHQRLTPTLALRAVCSGDVAFFTAALAVMA
                     GVPVTNAELLVNDPGGRGLVSLCDKAGVPAGLLPLIRAALEVTRGTGLDGGERDWERF
                     RARVIGRVLTQCEDLDPEDLDYLVERMPAAVAG"
     gene            complement(2444..>3167)
                     /locus_tag="VFN42_02360"
     CDS             complement(2444..>3167)
                     /locus_tag="VFN42_02360"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET8995488.1"
                     /translation="KGPSDFVSQADLRAEQTLKEELNKARPGYAFLMEESGASGSENW
                     AWRWVVDPLDGTTNFLHGMPHWAISIGLERRLPDGGSELAAGVIYAPTVDEMFWAEKG
                     IGAFVNERRLRVSARRDLASAVFATGIPFGGVSAARRQAFSRTLTALMPQVAGIRRFG
                     SAALDLAWVAAGRYDGYWELGIKPWDIAAGMLLVREAGGFVTGAHDGDPRDTAEIVAG
                     NAHLHAKLRDVVLEGMGAAPRG"
BASE COUNT          494 a         1060 c         1147 g          466 t
ORIGIN      
        1 tgacgctgaa ccggctgtgc ggctccggcc tgcaggccgt ggtgtccgcg gcgcaggcca
       61 tcaccctcgg cgacgccgac atcgcggtgg ccggcggtgc ggagagcatg agtcgggcgc
      121 catacatggc cagcggcatg cgctggggtg cccgcatggg cgacagcgcg atgatcgaca
      181 tgatgctggc tgcgctgcat gacccgttcg accacatcca catgggcgaa acggcggaga
      241 atctggcgga gagccaccaa atcacccgcg cgcagcagga cgctctggcc gagcagtcgc
      301 accagcgggc ggcgcgggca atcaaggaag gtcggttcaa gagccagatc ctgccggtga
      361 cgctgaagag ccgcaaaggc gagacggtgt tcgataccga cgagcatgtc cgcgccgaag
      421 cctccgccgc cgatctgggc aagctgcgcc cggtgtttcg taaggacggc acggtgaccg
      481 cgggcaacgc ctccggcatc aacgacggcg ccgccgcggt ggtgcttatg gacgccgaca
      541 ccgcgtcgaa gcgcggggcg aagccgctcg gccggctggt ggcctatgcg catgccggcg
      601 tggacccgaa gatcatgggc attggcccgg tgcccgcgac acgcaaggcg ctgcagcgcg
      661 ccgggctgac gctggcggac atggacgtga tcgagtcgaa cgaggccttc gcggcgcagg
      721 cctgtgcggt ggcgaaggac ctggggttcg atccggagaa gaccaatccg aatggatcgg
      781 gcatttcgct cgggcatccg gtgggtgcga ccggcacgat cctgacggtg aaggcgctgt
      841 atgaactggc gcggatcggc ggacgctacg cgctggtgac catgtgcatt ggcggcggcc
      901 aggggatcgc ggcgatcttc gagcgggtgt gagccggatt ctccgtccgc gctgtcggcg
      961 gacgacgatg cagtgcccgg cgccggccac ggtggattgg cgccgcatcg ccgcgcgcat
     1021 gcggggtctt cgggcggcga cgagccgtcg ctgatggccg gaaccgtggc ctgcccgatc
     1081 aggccgcggg tttcgtggaa ccggatcgcc gacaccaaga ggccggtgca ccaattaagc
     1141 cccgattaat ctgtgccgtc ctaaactccg tccggtgtgg acaggacgca cacgatgacg
     1201 gaggcgctca gcccgcggga tgtcgcgcgc ctgctgcagg aaccctctgc cgcaatgcgc
     1261 gccgaggtgg cggcgaaggt ggcgcggcag atcgacgatc cgcgcctgtc gccccaggaa
     1321 cttagcctgg cgcaggagat cgcccggatc atggcgcgcg acgtggcgct ggcggtacgc
     1381 cgcgccctgg cggacagcct gcgccgcgcc cgccgcctgc cgcgcgaggt cgcgctgcgg
     1441 ctggccgagg acatcgatgc tgtggcgctg ccgatcctgg ccgagtctcc cgtgctgacc
     1501 gacgacgacc tgctgcggat cctgcatcac gcatcgccgc gcaagcagga ggcgattgcg
     1561 gcacgtccga acctggcggc gccggtctgc gacgcggtgg ccgagagcgc ggaggaaccc
     1621 gcggtggccg cgctgatgcg caatccgacc gccagcctca ctctgcggag tttgcatcgc
     1681 gcgatcgatc gatttgccgc cagccaggca gtgcaggaga gcatcgtgca ccgtgcctcg
     1741 ctgccgatgg atatcgccga acggctggtg acggtgattt ccgaccggat gcgcgactat
     1801 ttggtcagcc accacgcgct gccttcggat tcagccgcgg acatcatact gcgcggccgg
     1861 gaacgcgcga tgctgcagtt gagccaggga gcggggcagg acgaattgct gcgcttgctg
     1921 cgtcagatgc actggcacca gcggctgact cccaccctgg cgttgcgggc cgtgtgctcc
     1981 ggggatgtgg cgttcttcac cgcggcgctg gcggtgatgg caggcgtgcc ggtgaccaat
     2041 gccgagttgc tggtgaacga tccgggcggc cggggcctgg tgtcgctgtg cgataaggcc
     2101 ggcgtgccgg cgggtctgtt gccactgatc cgtgcggcgc tggaggtgac gcgcggcacc
     2161 ggcctggatg gtggcgagcg cgattgggag cgcttccgcg cccgcgtgat cggccgcgta
     2221 ctgacgcagt gcgaggatct cgacccggaa gatctggact acctggtgga acgcatgccc
     2281 gccgcggtgg cgggttgaac gtggaccaaa ctcatccccc tccccttgcg agagggggct
     2341 ggggggaggg ggccgtcatg ggttccccgg gcaacctccc aggccccctc cccccatccc
     2401 cctcccgcaa ggggaggggg aggagtctct gtcccgcttg ccgtcaaccg cgcggcgcgg
     2461 cgcccatgcc ttccagcacc acgtcacgca gcttggcatg cagatgcgca ttgcccgcga
     2521 cgatctccgc ggtgtcgcgc ggatcgccgt catgggcgcc ggtgacgaaa ccgccggcct
     2581 cgcgcaccag cagcatgccg gcggcaatgt cccagggctt gatgcccagc tcccagtagc
     2641 cgtcgtaacg cccggccgcg acccaggcga ggtcgagagc ggcagagccg aagcggcgga
     2701 tgccggccac ctgcggcatc agcgccgtga gggtacggct gaacgcctgc cgccgcgccg
     2761 ccgagacgcc gccgaacggg atgccggtgg cgaataccgc actggccagg tcccgccgcg
     2821 cggagacacg cagccggcgc tcgttgacga aagcgccgat gcctttctcg gcccagaaca
     2881 tctcgtccac ggtcggggca tagatcaccc cagccgccag ctccgagccg ccgtccggca
     2941 gccggcgctc cagcccgatg ctgatcgccc agtgcggcat gccgtgcagg aaattggtcg
     3001 tgccgtccag cggatcgacc acccagcgcc aggcccaatt ctccgagccg gaggcgccgg
     3061 attcctccat caggaacgca tagccgggcc gagccttgtt cagctcctcc ttcagggtct
     3121 gctcggcccg caggtcggcc tgggaaacga aatcggaggg gcctttg
//
LOCUS       DASSZH010000117         3955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_821171, whole genome shotgun sequence.
ACCESSION   DASSZH010000117 DASSZH010000000
VERSION     DASSZH010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821171"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..451)
                     /gene="rplS"
                     /locus_tag="VFN42_02365"
     CDS             complement(44..451)
                     /gene="rplS"
                     /locus_tag="VFN42_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET8995489.1"
                     /translation="MNIIQQFEADEIARLTEGRAVPEFAPGDTVRVGVKVVEGERTRT
                     QTFEGVVIARSNKGLNSNFTVRKISYGEGVERVFPLYSPTIAEISVVRRGDVRRAKLY
                     YLRGRRGKSARIAEKARATGGAADNAADQAAAE"
     gene            complement(477..1202)
                     /gene="trmD"
                     /locus_tag="VFN42_02370"
     CDS             complement(477..1202)
                     /gene="trmD"
                     /locus_tag="VFN42_02370"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET8995490.1"
                     /translation="MTWRASVLTLFPEMFPGPLAHSLAGRALSNGLWSLEPKNIRDCA
                     TDRHRSVDDTPFGGGAGMVLRPDVLDAALQAVCDGRPALYLTPRGRRFTQADAQRYAA
                     APGLVLLCGRYEGVDQRVIEARHLEERSLGDFVLSGGEPAALAVLDATVRLLTGVMGA
                     AESATEESFSTGLLEYPHYTRPAEWQGRRVPEVLLSGHHAAVAAWRQEQAERITRERR
                     PDLWAQYLSANHSGPIASPAGAP"
     gene            complement(1199..1807)
                     /gene="rimM"
                     /locus_tag="VFN42_02375"
     CDS             complement(1199..1807)
                     /gene="rimM"
                     /locus_tag="VFN42_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010504002.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET8995491.1"
                     /translation="MPDSRILLGVIGRPHGVRGLVRVTSHTADPADLTAYGPLTDAQG
                     RRFVLRWHGEGLAEIAEVIGGTEVKVADRTAAEKLTNTRLYIDRAQLPQTEEDEFYLS
                     DLVGLAAADPAGAALGRVSAVHDYGAGASLEIERADAAPLLVPFTRACVPDINLAAGR
                     LTVSPPIEVDAPGDANRAPSALVGEERGGGSNDQTRPRSAAA"
     gene            complement(1815..2150)
                     /gene="rpsP"
                     /locus_tag="VFN42_02380"
     CDS             complement(1815..2150)
                     /gene="rpsP"
                     /locus_tag="VFN42_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET8995492.1"
                     /translation="MSLKIRLARAGAKKRPYYHIVVADSRSPRDGRFIEKLGSYNPML
                     PAEHEERIRLFNERIQHWVSQGALPTDRVARFLGKAGLGPMPAYREQPVKSAPKKKAQ
                     ERAKAAASA"
     gene            complement(2245..3630)
                     /gene="ffh"
                     /locus_tag="VFN42_02385"
     CDS             complement(2245..3630)
                     /gene="ffh"
                     /locus_tag="VFN42_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET8995493.1"
                     /translation="MFEALSDKLGGVFDRLRKRGALSEADIGEALREVRVALLDADVA
                     LPVVRDFIAKVRERAIGAEVLDSVTPGQQIVKIVNDAMIDALGGEGAVPLNLNAAPPV
                     PILMVGLQGSGKTTTAGKIALRLKDRQKRKVLLASLDTQRPAAQLQLAQLAEQAAVPS
                     LPIVAGETPVQIARRAMDTGRREGFDVVILDTAGRLSINQELMDEVRDVRAATSPAET
                     LLVVDAMTGQDAVNTAKAFNEALSITGVVLTRMDGDARGGAALSMRAVTGAPIKLVGV
                     GEKQDALEDFHPERVAGRILGMGDIVGLVEKASETIDQDEAEKLAAKMAKGKFDLEDY
                     AGQLRQITKMGSLSGIVGMLPGAQKMKAQLEDANLDTTFLKRQQAIISSMTKKERQKP
                     DIIKHSRKQRIAKGSGTTVQEINKLLKQFDDMTHMMKKMNKLGQKGLMRAGIGALLPK
                     HMQGGAGRRPF"
     gene            complement(3791..>3955)
                     /locus_tag="VFN42_02390"
     CDS             complement(3791..>3955)
                     /locus_tag="VFN42_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HET8995494.1"
                     /translation="ARNRKDHGPENLTIVRKLALNVLRTARPDLSIRRKRKRSGWSDA
                     FARSILGQMR"
BASE COUNT          548 a         1358 c         1360 g          689 t
ORIGIN      
        1 cgttccatca caccctctga gccgcaagga tccggattcc agactactcg gcggccgcct
       61 gatccgctgc gttgtccgca gcaccgccag tggcgcgcgc cttctcggcg atgcgcgccg
      121 acttgccgcg gcgtccgcgc aggtaataca gcttggcgcg gcgcacgtcg ccgcggcgca
      181 ccacgctgat ctcggcaatg gtgggcgaat acagcgggaa cacgcgctcc acgccctcgc
      241 cgtagctgat cttgcgcacg gtgaagttgc tgttcagtcc cttgttggaa cgggcgatca
      301 ccacgccctc gaacgtctgg gtgcgggtgc gttcgccttc caccaccttc acgccgacgc
      361 gcaccgtatc accgggggcg aactcgggca ctgcgcggcc ctcggtgagg cgggcgatct
      421 cgtcggcttc gaactgttgg atgatgttca tctgggtggt cctttcgtcc tgatccttag
      481 ggcgcgcctg ccggactggc aatcgggccg ctgtgattgg ctgacaggta ctgcgcccac
      541 aaatcggggc ggcgttccct cgtgatgcgt tcggcctgtt cctgccgcca ggcagcaacc
      601 gcggcgtgat ggccggagag cagcacctcc ggcacgcgcc gcccctgcca ctcggccggg
      661 cgggtgtagt gggggtattc cagcagcccg gttgagaagc tctcctccgt ggcgctttcc
      721 gccgcaccca tgacgccggt cagcaggcgg accgtggcgt ccagcaccgc cagggcggct
      781 ggctcgccgc cggacagcac gaagtcgccc agcgagcgtt cttcgaggtg gcgcgcttcg
      841 atcactcgct ggtccacgcc ttcgtagcgc ccacacagca gcaccaagcc cggggcggcg
      901 gcgtagcgct gggcatcggc ctgggtgaaa cggcgccccc ttggcgtgag atagagcgcg
      961 gggcggccgt cgcagaccgc ttgcagtgcg gcatcgagca cgtccgggcg cagcaccatg
     1021 ccggcgccgc cgccgaacgg cgtgtcgtcc acgctacggt ggcggtcggt cgcgcagtcg
     1081 cggatgttct tcggttccag cgaccacagg ccattgctca gggcgcggcc ggccagggag
     1141 tgggcgagcg ggccggggaa catctcgggg aaaagcgtca ggacggaggc gcgccaggtc
     1201 atgcggcggc actccgggga cgagtctggt cgttcgaccc cccacccctt tcctccccca
     1261 caagggctga aggggcgcgg ttggcgtcgc ctggtgcgtc aacttcgatc ggtggcgata
     1321 ccgtcaggcg accggctgcc aagttgatat cgggcacgca ggcccgagtg aacgggacca
     1381 gcagcggagc ggcatctgca cgctcgatct ccaggctggc gccggcgccg tagtcatgca
     1441 ccgcggagac gcggccgagc gcagcgcccg ccggatcggc agcagccagc ccgaccagat
     1501 cggagaggta gaattcgtcc tcctcggtct gcggcagttg ggcgcggtcg atgtacaggc
     1561 gggtgttggt cagtttctcc gccgcggtgc ggtcggccac cttcacttcg gtgccgccga
     1621 tcacctcggc gatctccgcc agtccctcgc cgtgccagcg caggacaaag cgccgcccct
     1681 gcgcgtcggt cagcggaccg tacgcggtca gatcggcggg atcggcggtg tgactggtca
     1741 cccgcaccag gccgcgcacg ccgtgcggcc ggccgatcac gcccaacagg atgcggctgt
     1801 ccggcacggt tcgctcaggc gctcgcggcg gccttggcgc gttcctgcgc cttcttcttc
     1861 ggcgccgact tcaccggctg ctcacgatac gcgggcatcg ggccgagccc ggccttgccg
     1921 aggaagcggg cgacacggtc ggtgggcagg gcgccctggc tgacccaatg ctggatgcgc
     1981 tcgttgaaca ggcgaatgcg ctcctcatgt tcggcgggga gcatcgggtt gtagctgccc
     2041 agcttctcga tgaaccggcc atcccgcggg ctgcggctgt cggcaaccac gatgtggtag
     2101 taggggcgct tcttggcgcc ggcgcgggcg aggcggattt tcaggctcat tggagtcggt
     2161 atctcctgtg caagtcgtgg gtggccggcc ttcgccggcc atgacgctgg ttgaacgggc
     2221 cggcctgcgc cggccacgac gaaactagaa aggccggcgg ccggcgccgc cttgcatgtg
     2281 cttgggcagc agcgcgccga tgccggcacg catcaggccc ttctgcccaa gcttgttcat
     2341 cttcttcatc atgtgcgtca tgtcgtcgaa ctgcttcagc agcttgttga tctcctgcac
     2401 cgtggtgccg gagcccttgg cgatacgctg cttgcggctg tgcttgatga tgtcgggctt
     2461 ctgccgttcc tttttcgtca tcgacgagat gatcgcctgc tggcgcttga ggaacgtggt
     2521 gtccaggttg gcgtcctcga gctgcgcctt catcttctgc gcgcccggca gcatgccgac
     2581 gatgccggac aggctaccca tcttggtgat ctggcggagc tgcccggcat agtcttccag
     2641 gtcgaacttg cccttcgcca tcttggcagc gagcttctcg gcctcgtcct ggtcgatcgt
     2701 ctcgctggcc ttctccacca ggccgacgat gtcgcccatg cccaggatgc ggccggcgac
     2761 gcgttcggga tggaaatcct ccagcgcgtc ctgcttctcg ccgacgccca ccagcttgat
     2821 cggcgcgccg gtcaccgcgc gcatcgacag cgccgcgccg ccgcgggcgt cgccatccat
     2881 gcgggtcagc accacgccgg tgatcgacag cgcctcgttg aacgccttgg cggtattgac
     2941 cgcgtcctgg ccggtcatcg catcgaccac cagcagcgtc tccgccgggc tggtggcggc
     3001 gcgcacgtcg cgcacctcgt ccatcagttc ctggttgatc gacaggcggc cggcggtgtc
     3061 caggatgacg acgtcgaacc cttcgcgccg gccggtgtcc atggcgcgcc gggcgatctg
     3121 caccggggtc tcgcccgcga cgatcggcaa actcggcacc gccgcctgct cggccagttg
     3181 cgccagctgc aactgggcgg cggggcgctg ggtgtccagg ctggccagca gcaccttgcg
     3241 cttctggcga tccttcaacc gcagcgcgat cttgcccgcg gtggtggtct tgccggagcc
     3301 ctgcaggccc accatcagga tgggcaccgg cggggcggcg ttcaggttca gcggaacggc
     3361 gccctcgccg cccagcgcgt cgatcatcgc gtcgttgacg atcttgacga tctgctggcc
     3421 gggggtgacg gaatccagca cttccgcgcc gatcgcccgt tcccgcacct tggcgatgaa
     3481 atcgcgtacc accggcaggg cgacgtcggc gtccagcagg gcaacgcgga cttcccgcag
     3541 cgcctcgccg atatccgctt cggacagggc gccgcgcttg cgcagccggt cgaacacccc
     3601 tccaagcttg tctgacagcg cctcgaacac gatcgcatca ctccaacggc agaagcgccg
     3661 ctgcgcgaac cctcgcggag cggcggagcc cccgcatgcg gcggggaccg ggtgaccctg
     3721 gtgacccgga ggccggggtt atgggcagcg gcggccggcg agtcaatgcc ggtttccgga
     3781 aggtccaggg ctatcgcatt tggccgagga tggatcgggc gaaggcgtcg gaccatccgg
     3841 agcgcttgcg cttgcggcga atggagaggt ccggccgggc ggtgcgcagg acgttcagtg
     3901 ccagtttccg cactatggtg aggttctccg gcccatgatc cttgcggttt cgcgc
//
LOCUS       DASSZH010000118        11594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1059752, whole genome shotgun sequence.
ACCESSION   DASSZH010000118 DASSZH010000000
VERSION     DASSZH010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 11594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11594
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059752"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1484
                     /locus_tag="VFN42_02395"
     CDS             <1..1484
                     /locus_tag="VFN42_02395"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HET8995495.1"
                     /translation="NRRGRWVAGGVAGGVLGASVVAAFAAGIAGAFQGNGQELLNAGV
                     LCLAVCMLGWHNIWMARHARELAANASHVGREVASGARPLAALALITGAAVLREGSET
                     VLFVFGILAGTQTGLAGLLAGGLLGLAAGAAAGAALYLGLLRIPLHRLFQVTSWMVLL
                     LAAGLAAQAAAFLVQANLLPPLGNAVWDTSWLLSEDSIPGRVLHTLIGYVAQPEGIQL
                     IAFIATLLAIGVPMKLISRPRKTAVAMAVLLGIGMLGLPHAAHAELRVFMPNAEYREV
                     EFEHNGLVEFGAKGSGFDHAQSYVNALGYGALPWWHMELEGEMASGAGQSLYWTANTL
                     ENVFQLNEPGEYALNLGFFAEYSQSALRGVANSITAGPIVQKEFSDTFGLDTLHTLNL
                     FLSHDLGHDSTRATGLDISWQSVVRWKPLIAPGFEYYGIIDDIAHAGPFARQQHLIGP
                     VLTGSHNFAPFGTLKYEVGYLFGLTPGSPRGAVRWRLEYELAF"
     gene            1619..1873
                     /locus_tag="VFN42_02400"
     CDS             1619..1873
                     /locus_tag="VFN42_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HET8995496.1"
                     /translation="MSRWPVQDAKARFSEMLDTCLQQGPQVVTRRGLDAAVLVPFDQW
                     QRLQAGKPTLKALLLAAEPRADIPLPPRGQRRRRSPAAFT"
     gene            1879..2283
                     /locus_tag="VFN42_02405"
     CDS             1879..2283
                     /locus_tag="VFN42_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HET8995497.1"
                     /translation="MYLLDTNVVSELRKRRPHPAVIAWLEPLADNELFLSAVTIGELQ
                     AGVEITRMQDPAKAAEIEGWVDQVAETWNVLPMDARAFRAWARLMHGRGDELLEDAMI
                     AATAQVNKLTVVTRNLRDFVPFGVSTLDPFAS"
     gene            complement(2323..3465)
                     /locus_tag="VFN42_02410"
     CDS             complement(2323..3465)
                     /locus_tag="VFN42_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006558332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET8995498.1"
                     /translation="MLRLARRASLARTIGCLALLTTLAACASHGPSVSQSAETARYLA
                     RAHGNYAPPGPPNDPWGPYIHEAADRFHIPGRWIRAVMHVESGGQEYMHGQLTTSGAG
                     AMGLMQVMPETYDELRQRYNLGDDPFNPHDNIMAGAAYLREMYDIYGSPGFLAAYNAG
                     PGRLDDYLANNRPLPAETRHYVAMIGPEIIGVEPAQRSPAEQYAMNQLPVYIPPGKRY
                     SHPVQLAGRREGGGGRLPAPRPIQVAQLPEPPRAVPPAPASAIALVTPPPPPPAQGGF
                     HLIQRAVAEPIPFHHVPAASGRWAIQVGAFSSQADARKAIGLARRHAHVELAVAHSAV
                     EMVHSGHSVLWRARLTGLSRDTAAEVCGRLTRGRMACMLLSPEGQS"
     gene            3688..4053
                     /locus_tag="VFN42_02415"
     CDS             3688..4053
                     /locus_tag="VFN42_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995499.1"
                     /translation="MQRFFVSACLLGSLAGCTAAPPPPPVVAHVSPPGEAGIVLAIHP
                     VPAAQSGPAELVLARLGGGPGLAGNAEFVVRRADGAVFAVVQPRVQQDAATLRPGSRV
                     LILPGIPTRLRALPALAAR"
     gene            4190..5044
                     /locus_tag="VFN42_02420"
     CDS             4190..5044
                     /locus_tag="VFN42_02420"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8995500.1"
                     /translation="MSTALSFTAKNALVEFKADQTFSSRNGLASADVADGIRLLPLAW
                     TLGWLDIRLRYRGSVLGPFWLTLSTGVMVAALGYLYSGIFHTDIHDYLPFLALSMVLW
                     TFLSTVISEACLAFTEAEGVIRAVRMPYFLFGIRILLRNMLVLAHNVLVIVVVYLVFS
                     LWPGWHLLLAVPGVLLWCVDSLALVMLLGGFCARFRDIPPIVNSVMQIAFFITPVLWK
                     PDQLGAGARLLPLNPFFDLLTVVRAPLLGSVADANVWLGALLYSALIVALAWLFFTRA
                     RGRIAFWI"
     gene            5062..5844
                     /locus_tag="VFN42_02425"
     CDS             5062..5844
                     /locus_tag="VFN42_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8995501.1"
                     /translation="MSDAQILVEDVSVFFPLYHANARSLKKTVMAAASGRLGKDKQRR
                     VVVQALRDINFVLNGGDRLGLIGSNGAGKTTLLRTLAGIYEPVAGRVRVQGSLNALLD
                     AGLGMNMDLTGRENIMLRGLYNGLPRADLARLEEDVAEFAGLGDFLDLPVRIYSAGMV
                     VRLGFALATAIRPQILLMDEWLLAGDAEFMERARHRLEAMVRSAEILVLSSHDIGIVR
                     NWCTRVMWLDHGRIKADGPADAVADLYSGPKPVPEAIEAVAG"
     gene            complement(5879..6640)
                     /gene="hypB"
                     /locus_tag="VFN42_02430"
     CDS             complement(5879..6640)
                     /gene="hypB"
                     /locus_tag="VFN42_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HET8995502.1"
                     /translation="MCDSCGCATGGTQVVVGGHRIVPPRDDDAAVLTRLLAENDGIAL
                     HNREHIEDHGVLAINLMSSPGAGKTALLEATIEALHREFRFGVIEGDLETDNDAVRIR
                     RHHVPVVQITTGNACHLDAGMVHEALHELPLDKLDILFIENVGNLVCPAAFALGQHRN
                     VTLLSATEGDDKPAKYPVMFRTSHLVLISKADLLPHLDDFDPARATEAVRRLANDAPV
                     FTLSARRHTGMAPWADWLRSERLLLRSRLELAAAD"
     gene            complement(6633..7070)
                     /locus_tag="VFN42_02435"
     CDS             complement(6633..7070)
                     /locus_tag="VFN42_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HET8995503.1"
                     /translation="MPSRCRPSGRLGERELHAGTGATRVHELSLCRALLKLVEPIALQ
                     HAAPVVRVVLRVGPLSGAEPELLMRAFPQAAAGTCADGAQLDLQLTPLRISCLVCGAE
                     SDAAVNRLACTVCGATRTRLLSGDELDLLRVELHPLEANESHV"
     gene            complement(7197..7424)
                     /locus_tag="VFN42_02440"
     CDS             complement(7197..7424)
                     /locus_tag="VFN42_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995504.1"
                     /translation="MSASIQSAPLPTQLRALRVGMDDSLHIRRTVQLAAAKIEDLEDQ
                     VARLAEELRRSAGFPGNARRAKRDMRPPRLS"
     gene            7740..8492
                     /locus_tag="VFN42_02445"
     CDS             7740..8492
                     /locus_tag="VFN42_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HET8995505.1"
                     /translation="MDNQTSDAPVCGIEGCDDNSATPASLHHDNAVGTRIDIVSDAIC
                     PWCFIGKRQLERALAILEPEGLRFSIHWNPFQLNPDMPREGRDRAAYRAWKFGGAERA
                     KAADDRVAEAAERVGLRFRQDLMLRTPNTLDAHRLIWFAGQKGVQDAVMETVFAAYFL
                     QGRDIGDRAVLADCAGQAGLDRDEAAQFLAGDLAEREMLAADQAAREAGVNGVPSFFL
                     DGYGLLSGAVPAEQMAEALRRGNAVLRERAAD"
     gene            complement(8625..9905)
                     /locus_tag="VFN42_02450"
     CDS             complement(8625..9905)
                     /locus_tag="VFN42_02450"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HET8995506.1"
                     /translation="MTATERDARGRPVVVVTGMGVLTSLGRGQAENWRKLTAGESGLR
                     RISRFPVEGLRTTVAGTVDYVQLDEPSAPALAEAMAALAIEEALAEAGAGRPGDFPGP
                     LFLALPPVEIEWTQRRALAAAAGNGAVTYAGLLRAAGSGAFTRWNRRFQFGSVGETLA
                     DRFGTKGSPISTSTACASGGTAIQLGLEAIRRGETEAALCVGTDASVTVESLIRFSLL
                     SALSTRNDPPSSAARPFSKDRDGFVMAEGAGALVLESLAHARARGATILGVIEGCGEA
                     ADGFHRTRSSPDGKPIIACIARALADAGLEPDAVGYINAHGTGTPENDKMEWVGVSAV
                     FGERAGSIPISSNKSMIGHTLSAAGALEAIFTVLSLRHGRLPPTINYDIPDPALPVDC
                     VPNVARDARVAHAISNSFGFGGQNVCLAIGAEPG"
     gene            complement(10082..11269)
                     /locus_tag="VFN42_02455"
     CDS             complement(10082..11269)
                     /locus_tag="VFN42_02455"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005084.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HET8995507.1"
                     /translation="MAERDALITGIGLVSCLGEGVEAHWAALNQPGGFQPVVDAGTFA
                     PWPVHPMVPLELDKQIPKRGDQRQMESWQRIGTYAAGLALDSAGLKGNAEVLGRTDMI
                     VAAGGGERDYAVDEAILSALPGAGDPDLLLNQRLMTDLRPTLFLAQLSNLLAGNISIV
                     HGVIGSSRTFMGEEASGADAVRIACARIAAGQGDVFLVGGSFNAQRPDALMLHELGGT
                     LWKQAYAPVWERQAAGGGMVLGSLGCFLVLESREHAAARGVPAMARVAAIATDRCRRG
                     AGEATAVARRQYAGMRHHLQDGAAAVISGTSGAAAASAEEAGFLAELGLPVRAAVTAF
                     GHAMEPSFLGSLALAAISVRQGTLFPPLESAEAAMPGALRQALVTSWGWWRGEAMAVV
                     DGV"
     gene            complement(11262..>11594)
                     /locus_tag="VFN42_02460"
     CDS             complement(11262..>11594)
                     /locus_tag="VFN42_02460"
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hydroxyacyl-ACP dehydratase"
                     /protein_id="HET8995508.1"
                     /translation="GVLMIETMAQTGGWLIMAAQRFANMPFLLQVEKAKLRSFVTPGQ
                     PLQAEAELVHEGSGYAVVNGRLASGGKPVASAEIRYRVVPFPNPTLRAQMLETARRVA
                     VPEAFLHG"
BASE COUNT         1767 a         3959 c         3980 g         1888 t
ORIGIN      
        1 tcaaccggcg gggccgctgg gtggctggcg gcgtggccgg cggcgtgctg ggcgcctcgg
       61 tggtggccgc gttcgcggcc ggcatcgccg gcgcgttcca gggcaacggg caggagctgc
      121 tgaacgcggg cgtgttgtgc ctcgcggtgt gcatgctggg ctggcacaac atctggatgg
      181 cccgccacgc ccgcgagctg gcggccaatg ccagccatgt cgggcgggag gtcgcgtccg
      241 gcgctcgccc cctggccgca ctggcgctga tcaccggcgc cgccgtgctg cgcgaaggct
      301 cggagaccgt gctgttcgtg ttcggcatcc tggccgggac ccagaccggc cttgccggcc
      361 tgctggcggg cggtctgctc ggcctggccg caggtgccgc agcgggcgcg gcgctctatc
      421 tgggcctgct gcggataccg ctgcaccggc tgttccaggt gaccagttgg atggtgctgc
      481 tgctggccgc cggcttggcg gcgcaggcgg cggccttcct ggtccaggcc aatctgctgc
      541 cgcccttggg caacgcggtc tgggacacct cgtggctgct gtcggaggac agcatccccg
      601 gccgcgtgct gcatacattg atcggctatg tcgcgcagcc ggaaggcatc cagctgatcg
      661 ccttcatcgc cacgctgctt gcgatcggcg tcccgatgaa actgatcagc cggccccgca
      721 agactgcggt ggccatggcg gtgttgctgg gcatcgggat gctgggcctg ccgcatgccg
      781 cacatgccga gctgcgggtg ttcatgccga atgctgagta ccgcgaggtg gagttcgagc
      841 ataacggcct ggtggagttt ggcgcgaaag gctccgggtt tgatcatgcg cagagctacg
      901 tcaatgccct tggctatggc gctctgccgt ggtggcacat ggagctggaa ggggaaatgg
      961 cgtcaggtgc cggccaatcg ctgtattgga cggcaaatac gctggagaac gtgttccagc
     1021 tgaacgagcc cggcgagtat gccctgaacc tggggttctt tgccgaatat tcgcagtcgg
     1081 cactgcgcgg cgtggccaac agcatcactg ccgggccgat cgtgcaaaag gaattcagcg
     1141 acacgttcgg gctggatacg ctgcacacgc tgaacctgtt cctgtcgcac gatctcggac
     1201 atgattcgac gcgggcaacc gggctggaca tttcctggca gtcggtggtg cggtggaagc
     1261 cgctgatcgc gccggggttc gagtattacg gcatcatcga cgacatcgcg catgcggggc
     1321 cgttcgcgcg gcagcagcac ctgatcggcc cggtgctgac cggcagccac aacttcgcgc
     1381 catttggcac cctgaaatac gaagtcggct acctgtttgg tctgacaccc ggttcaccgc
     1441 gcggcgcggt gcggtggcgg ctagaatacg aactggcgtt ctgatcggcg aggttcgtgt
     1501 tacgcatctt ccttgacaac cattagatga ctagctatct gactagtcat ccaagggcgg
     1561 cgccaccgca atcgcaaagc cgggcgcccg cttaacgctt tggcaatgga aggccgctat
     1621 gtcccgctgg cccgtgcagg acgcgaaagc gaggtttagt gagatgttgg acacctgtct
     1681 ccagcaaggt ccgcaggtgg tgacccgccg cggactggat gccgccgtgc tggtgccttt
     1741 cgaccaatgg cagcggcttc aggccggcaa gccgacgctc aaggcgctgc tgctcgccgc
     1801 cgaaccgcgg gccgatattc cgctgcctcc acgtggccag cggcgccggc gctcaccggc
     1861 cgccttcacc tgacccaagt gtacctgctg gacaccaacg tcgtctcgga gctgcgcaag
     1921 cggcgcccgc atcccgcggt gatcgcctgg ctggaaccgc tcgccgacaa cgagctgttc
     1981 ctgtccgccg tcaccatcgg cgaattgcag gcgggggtgg agatcacccg catgcaggat
     2041 cccgccaaag ccgcggagat cgagggctgg gtcgatcagg tagcggaaac ctggaacgtg
     2101 ttgccgatgg acgcacgggc cttccgtgcc tgggcgcggc tgatgcatgg ccgcggcgac
     2161 gagctgctgg aagatgccat gatcgccgcc accgcgcagg tgaacaagct gacagtggtc
     2221 acccgcaatc tgcgcgactt cgtgcccttc ggagtcagca cgctcgaccc gttcgcgagc
     2281 tgacccgcag gccgcgcggt cacccggagc cgattcgccg gttcaggact ggccttccgg
     2341 cgacagcagc atgcaggcca tgcgcccgcg ggtcagccgg ccacagacct ccgccgcggt
     2401 atcgcgcgac agtccggtca ggcgggcgcg ccacaggacc gagtgcccgc tgtgcaccat
     2461 ctccaccgcc gaatgcgcca ccgcgagctc cacatgggca tgccggcgcg ccagaccgat
     2521 cgccttgcgc gcatccgcct ggctgctgaa cgcaccgacc tggatcgccc agcgcccact
     2581 tgccgccggc acgtggtgga acggaattgg ttcggccacc gcacgctgga tcaggtggaa
     2641 gccaccctga gccgggggcg gaggcggtgg cgtgaccaag gcgatcgcgc ttgccggggc
     2701 cggcggtacc gcgcgcggtg gctcggggag ttgcgccact tggatcggcc gcggggcggg
     2761 caggcgtccg ccgccaccct cacgccggcc ggcgagctgc accggatggc tgtagcgctt
     2821 gccgggcggg atgtacaccg gcagctggtt catcgcgtat tgttcagccg gggaacgctg
     2881 cgccggctcc acgccgatga tctccgggcc gatcatcgcg acatagtgcc gcgtttccgc
     2941 cggcagcggc cggttgttgg cgagataatc gtccagccgg cccgggcccg cattgtaggc
     3001 ggccaggaat ccgggcgatc cgtagatgtc gtacatctct cgcagatacg ccgcgccggc
     3061 catgatgttg tcgtgcggat tgaacggatc gtcgcccaga ttgtagcgct ggcggagttc
     3121 gtcatacgtc tccggcatca cctgcatcag ccccatcgca ccggcgccag aggtggtcag
     3181 ttggccgtgc atgtattcct ggccgcccga ctccacgtgc atcaccgccc gaatccaccg
     3241 cccggggata tggaagcggt ccgcggcctc gtggatatac gggccccagg ggtcattcgg
     3301 cggccccggc ggggcgtaat tcccgtgcgc cctggcaaga tagcgcgccg tctccgccga
     3361 ctggctgacg cttggcccgt gcgaggcaca ggcggccagg gtcgtcagca aagccaggca
     3421 gccgatggtg cgagccagag aggcacggcg ggcgaggcgg agcatccggt agagcctccg
     3481 tcgtggcgcg gctgaatagc cggagggcac gacgaccgga cggccgtcgc taaggtggac
     3541 gatcaggaac agcgcctaac ccattatcgc aaatcgctga tccaggcaag tcatctcgcc
     3601 gcccggaatg tgtcgcacat tcacttgtcc gcctgcagcg aatcccgcag gatcatgcct
     3661 ccccctgaac gacagggatg cagaacattg cagcggtttt tcgtatcggc ctgtctgttg
     3721 ggaagcctgg ctggttgcac cgcggcgccg ccgcctccgc cagtggtggc gcatgtcagc
     3781 ccgcccggtg aggccggcat cgttctcgcg atccacccgg taccggcagc ccagtccggg
     3841 ccggccgaac tggtgctggc ccggctgggc ggaggccccg gcttggcggg gaatgcggaa
     3901 ttcgtggtac gccgggcaga tggcgcggta ttcgccgtgg tgcagccacg cgtgcagcag
     3961 gacgcggcga cattgcggcc gggcagccgg gtgctgatcc tgcccggcat cccgacccgc
     4021 cttcgcgcct tgccggcgct ggccgcgcga tagcaggccg ccggcttcgc cagggcgggc
     4081 gctttcctgg ggaatcggca ccgcggcgac cacacattcg gcatcgccgc ccccgcgcgc
     4141 cgcttggcga ttgcgggggc agggcggatg cggtatccgc ccggcaccca tgagcaccgc
     4201 tctctccttc acggccaaga acgcgcttgt cgagttcaag gcagaccaga cattcagctc
     4261 ccgcaacgga ttggcgtcgg cggacgtcgc ggatggcatc cggctgctgc cgcttgcctg
     4321 gaccctcggc tggctcgata tccggctgcg ctatcgcggg tcggtgctcg gtcccttctg
     4381 gctgaccctg tcaaccggcg tgatggtcgc ggcgctcggc tatctctatt ccggaatctt
     4441 ccacaccgat atccacgact atctgccgtt cctggcgctc tcgatggtgc tgtggacctt
     4501 cctcagcacg gtgatttccg aggcgtgcct ggcgttcacc gaagcggaag gcgtcatccg
     4561 tgcggtgcgc atgccgtact tcctgttcgg catccgcatc ctgctgcgca acatgctggt
     4621 gctggcacac aatgtgctgg tgatcgtcgt ggtgtacttg gtgttctcgt tgtggccagg
     4681 ctggcatctg ttactcgctg tccccggcgt gctgctctgg tgcgttgatt ctctcgccct
     4741 ggtcatgctg ctcggcggct tctgcgcgcg cttccgcgac atcccgccaa tcgtcaacag
     4801 cgtgatgcag atcgccttct tcatcacccc cgtgctgtgg aagccggatc agcttggtgc
     4861 cggagccagg ctgctgccgc tgaatccgtt tttcgacctg ctgaccgtgg tgcgcgcgcc
     4921 gctgcttggc tccgtcgccg atgccaatgt ttggctcggc gcactgctgt acagcgccct
     4981 gatcgtcgcg ctggcgtggc tgttcttcac ccgcgcccgc gggcgcatcg ccttctggat
     5041 ttgagcgtgg aaccgccggc cgtgagcgac gcacaaatcc tggtcgagga cgtttcggtc
     5101 ttcttcccgc tgtaccacgc caatgcgcgc agcctgaaaa agaccgtgat ggccgctgca
     5161 tccggccggc ttggaaagga caagcagcgc cgcgtcgtgg tgcaggcgct gcgggacatc
     5221 aacttcgtgc tgaatggcgg tgaccggctg ggtctgattg gctccaacgg cgccggcaag
     5281 accacgctgc tgcgcacgct ggccggcatc tatgaacccg tcgccggcag ggtgcgcgtg
     5341 caaggcagcc tgaacgcgct gctcgatgcc ggcctcggca tgaacatgga cctgaccggg
     5401 cgtgagaaca tcatgctgcg cgggctgtat aacgggctgc cccgagcgga cctggcgcgg
     5461 ctggaagagg atgtggcgga gttcgccggg ctgggcgatt tcctcgacct gccggtacgg
     5521 atctattccg ccggcatggt ggttcgcctc ggcttcgccc tggcgaccgc gatccggccg
     5581 cagatcctgt tgatggatga atggctgctc gccggcgacg cggagttcat ggagcgcgcc
     5641 cggcaccgcc tggaggcgat ggtacgcagc gcggagatcc tggtgctgtc gtcgcacgac
     5701 attggtatcg tgcgtaactg gtgcacgcgg gtgatgtggc tggaccacgg ccgcatcaag
     5761 gccgacggcc cggccgacgc ggtcgccgat ctgtattccg gcccgaagcc ggttccggag
     5821 gcgatcgaag ccgtcgcggg ttgagcacgt ggcggtgttc cgccgcgcga cgccccgatc
     5881 agtcggccgc cgccagttcc agccgcgacc gcagcagcaa acgttccgaa cgcaaccagt
     5941 ctgcccaggg cgccattccg gtgtgccggc gcgccgacag cgtgaacacc ggcgcatcgt
     6001 tggccagtcg gcggaccgcc tcggttgcgc gtgccggatc gaaatcgtcc agatgtggca
     6061 gcaggtccgc cttgctgatc agcaccagat gggaggtgcg gaacatcacc ggatattttg
     6121 ccggcttgtc gtcgccttcg gtggccgaca gcagcgtgac gttgcgatgc tggccgagtg
     6181 cgaaagccgc gggacagacc aggttgccga cattttcgat gaacaggata tcgagtttgt
     6241 ccagcggcag ttcgtgcaac gcctcgtgca ccatgccggc atccaggtgg caggcattgc
     6301 ccgtcgtgat ctgcaccacc gggacatggt ggcgccggat gcgcaccgcg tcgttgtcgg
     6361 tttccaggtc gccttcgatc acgccgaagc gaaactcgcg gtgcaatgcc tcgatcgtgg
     6421 cttccagcag cgcggtcttg ccggcgccgg gtgaggacat caggttgatt gccaacacac
     6481 catgatcctc gatgtgttcc cggttatgca gggcaatgcc gtcgttttcg gccagcagcc
     6541 gggtcagcac cgctgcgtca tcgtcgcgcg gcggcacgat gcggtgcccg ccgaccacga
     6601 cctgggtgcc gccggtggca cagccgcaag agtcacacat gggattcgtt cgcttccaaa
     6661 ggatgcagtt cgacgcgcag gagatccagc tcgtcaccgc tgagcagccg cgtccgcgtc
     6721 gcgccgcaaa ctgtgcaggc cagacgattg accgccgcat cgctttcggc gccacaaacc
     6781 aagcagctta tccgcagcgg cgtcagctgc aggtcgagtt gggcgccgtc ggcacaggtg
     6841 ccggccgctg cctggggaaa tgcccgcatc agcagctccg gttcggcccc ggagagtggc
     6901 cccacgcgca gcaccacacg gacgaccggc gccgcatgtt gcagggcaat cggttccacc
     6961 agcttcagca aggcgcggca aagcgagagt tcatgcaccc tcgtcgctcc agtcccggca
     7021 tgcagttccc gttcgccaag ccgccctgag gggcggcaac gagacggcat cctagatggg
     7081 tatcgccatg gacaggttga tctgcatcaa gcgcctgccg ccacgcgacc gcaccgggcg
     7141 ccgtcgtccg cagtcaccct gctgcggcgg cgccggaatc gcgcctcagc gcgcggtcag
     7201 ctcagccgcg ggggacgcat gtcccgcttg gcacggcgcg cattacccgg gaaaccggcg
     7261 ctgcggcgca gctcttccgc cagacgggca acctgatcct ccagatcctc gatcttggcc
     7321 gcggcaagtt ggacagtgcg gcgaatgtgc agactgtcat ccatcccgac gcgcagggcg
     7381 cggagctggg tcggcaatgg ggcagattgg atcgaggcgc tcattcagtc cctccaggcc
     7441 cggatgttct cagacagctc ggcaaaactt cggccggcct tgccgcccgg tctgccagat
     7501 agatgttgag aaacagttaa cgcgccctgc gtgtgtcttc agtacgcgga agccgtggat
     7561 cacgtcctgt tgcccagtgc ctggtaacgc ctgtgcgagg cagctttccc tgcatctgcc
     7621 gcgcaccact cctggtgccg aacagtacag cgctcgccca gctcgctgac cgcggaccgg
     7681 cggcatgcat tgccgggcgg ccgcgatgac gccatgttgc gtcccggcag gaggacatca
     7741 tggacaatca gaccagcgac gccccggtat gcggcatcga aggctgcgac gacaacagcg
     7801 ccacgccggc ctcactgcat cacgacaacg cagtgggcac gcggatcgac attgtctccg
     7861 acgcgatctg tccgtggtgc ttcatcggca agcgccaact ggagcgtgcg ctggcgatac
     7921 tggagccgga gggcctgcgg ttctccatcc actggaatcc cttccagcta aacccggaca
     7981 tgccgaggga gggccgcgac cgcgcggcct accgcgcctg gaaattcggt ggggcagagc
     8041 gggcaaaagc cgccgacgac cgcgtggccg aagccgccga gcgggttggg ctgaggttcc
     8101 gccaggacct gatgctgcgc accccaaaca cgctggatgc acaccggctg atctggttcg
     8161 ccgggcaaaa gggcgtgcag gacgcggtca tggagacggt gtttgccgcg tacttcctgc
     8221 aaggccgcga tatcggcgac cgcgcggtgc tggcggactg cgccgggcag gctggactgg
     8281 atcgggacga agcggcccag ttcctcgccg gcgacctcgc cgagcgggag atgctggccg
     8341 cggaccaggc ggcgcgggag gcgggggtga acggcgtgcc atccttcttc ctggacggct
     8401 acggcctgct ctccggtgca gtaccggccg agcagatggc cgaggcgctg cggcggggga
     8461 acgccgtcct gcgggagcgc gcggccgatt agccaggatg tccgcatggc atcatgccgg
     8521 cgttcttggc ctggctggag gcaacacgcc tgcgggcggc ctcgccggag cgcttcgcag
     8581 gtcatccgcc ggccgggcga agatggccca gccggaaagc tgggtcaccc cggctctgct
     8641 ccgatcgcca ggcagacgtt ctgcccgccg aagccaaacg aattggaaat cgcatgcgcc
     8701 acccgggcgt cccgcgcgac attcgggacg cagtccaccg gcagcgccgg gtcgggaatg
     8761 tcgtagttga tcgtcggcgg cagccggccg tgacgcaggc tcagcacggt gaagatcgcc
     8821 tccaacgccc cggcggcact cagcgtatgg ccgatcatcg acttgttcga ggagatcggg
     8881 atgctgccgg cgcgctcgcc gaacacggcg gagacgccga cccattccat cttgtcgttt
     8941 tccggcgtgc cggtgccgtg cgcgttgatg tagcccaccg cgtccggttc cagcccggca
     9001 tccgccaggg cgcgcgcaat gcaggcgatg atcggcttcc cgtccggtga ggaccgggtg
     9061 cggtggaagc cgtccgccgc ctcgccgcag ccctcgatca cccccaggat ggtggcaccg
     9121 cgggcgcggg catgggccag gctctccagc accagcgcgc cggcgccttc cgccatcacg
     9181 aacccgtcgc ggtccttgct gaacgggcgc gcggcggagg acggggggtc gttgcgggtg
     9241 gacagcgcgg acagcagtga gaagcggatc aggctctcga cggtcaccga ggcgtcggtg
     9301 cccacgcaca gcgccgcctc cgtctcgccg cggcggatcg cctccaggcc aagctggatc
     9361 gctgtgcccc cggacgcgca ggcggtggag gtgctgatgg gagagccctt ggtgccgaag
     9421 cggtccgcca gggtctcccc caccgagccg aactggaagc ggcggttcca gcgggtgaac
     9481 gcgcccgatc cggcggcacg cagcaggccg gcataggtca ccgcgccgtt accggcggcg
     9541 gcggccaagg cacgccgctg ggtccactcg atctccaccg gcggcagggc caggaacagc
     9601 gggccgggaa agtcgcccgg gcggccggcg ccggcttccg ccagcgcctc ctcgatcgcc
     9661 agcgcggcca tggcttccgc cagggccggg gcggagggtt catccagttg cacgtagtcc
     9721 acggtgccgg cgacggtggt ccgcagaccc tcgaccggga accggctgat ccggcgcagg
     9781 cccgattcgc cggcggtcag cttccgccag ttctccgcct ggccgcgccc cagcgaggtc
     9841 agcacgccca tcccggtgac caccaccacc ggccggccgc gcgcgtcgcg ttctgtggct
     9901 gtcatgctgt gcgtgtcctt aaccgcgaga cgaggcaggg aggggcgaat gacgattccc
     9961 cctccccttg cgggacgggg tcagggggag ggggcggtgg aggttgccag gggaaatcat
    10021 gcgggccccc tacccctgcc ccctccggca cggggagggg gagattctgt gccgcggtgc
    10081 ctcaaacccc atccaccacg gccatggcct caccgcgcca ccagccccag gaggtcacca
    10141 gtgcctggcg cagcgcgccc ggcattgctg cctcggcgga ctccagcggc gggaacagcg
    10201 tgccctgccg caccgaaatc gccgccagcg ccaggctgcc caggaaagac ggctccatcg
    10261 cgtgtccgaa cgccgtcacg gcggcccgca ccggcagacc gagttcggcc aaaaagccgg
    10321 cctcctccgc gctggccgcc gccgcacctg acgtcccgga aatcaccgcc gccgcaccat
    10381 cctgcaggtg gtgccgcatg ccggcgtatt gccggcgcgc caccgcggtg gcctcgcccg
    10441 caccgcggcg gcagcggtcg gtggcgatag cagccacacg cgccatcgcc ggcaccccgc
    10501 gtgccgcggc gtgctcccgg ctttccagca ccaggaaaca gcccagcgag ccgagcacca
    10561 tgccgccgcc cgccgcctgc cgttcccaga ccggcgcata ggcctgcttc cagagcgtac
    10621 cgcccagctc atgcagcatc agcgcatccg gccgctgggc gttgaacgac ccacccacca
    10681 ggaacacgtc gccttgcccc gcggcgatgc gtgcgcaggc gatgcgcacc gcgtcggcgc
    10741 cggatgcctc ctcgcccatg aaggtgcgcg aggagccgat caccccgtgc acgatggaga
    10801 tgttgcccgc cagcaggttg gacaactgcg ccaggaacag cgtcggccgc aggtcggtca
    10861 tcagccgctg gttcagcagc aggtccggat cgccggcgcc gggcagggcg gacaggatcg
    10921 cctcgtccac cgcgtagtcg cgctctccgc cgcccgccgc cacgatcatg tcggtgcggc
    10981 ccagcacctc ggcgttgccc ttcaggccgg cggagtcgag ggccaggccg gcggcgtagg
    11041 tgccgatgcg ctgccatgac tccatctgcc gctggtcgcc gcgcttgggg atctgcttgt
    11101 ccagctccag cggcaccatc gggtgcaccg gccagggggc gaaggtgccc gcgtccacca
    11161 cgggctggaa gccgccgggc tggttgagtg ccgcccagtg cgcctcgacg ccttcgccca
    11221 ggcaggacac caggccgatc ccggtgatca gcgcgtcgcg ctcagccatg caggaatgcc
    11281 tccggcaccg ccacgcgccg agcggtctcc agcatctggg cgcgcagcgt cgggttgggg
    11341 aagggcacca cgcggtagcg gatttccgcg ctggccacgg gcttgccgcc ggacgccagc
    11401 ctgccgttca ccacggcata gccggagcct tcgtgcacca gctcggcctc ggcctgcagc
    11461 ggctggcccg gggtgacgaa ggagcgcagc ttggccttct ccacctgcag caggaacggc
    11521 atgttggcga agcgctgcgc tgccatgatc agccagccgc cggtctgcgc catggtctcg
    11581 atcatcagca cgcc
//
LOCUS       DASSZH010000119         7510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1042998, whole genome shotgun sequence.
ACCESSION   DASSZH010000119 DASSZH010000000
VERSION     DASSZH010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7510
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042998"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VFN42_02465"
     CDS             <1..389
                     /locus_tag="VFN42_02465"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HET8995509.1"
                     /translation="AIEALQKGGQQMSRTMAQQFGSGQQGEEADGEDGGEGQMGMQDQ
                     QGEGEGSGSLYGRRGRQDGRGRDPLGRELGQGSSGTDESNDVQIPEKREWQRTRQIEN
                     ELRQRGAERDRPQQELDYIERLLKQF"
     gene            complement(444..1514)
                     /locus_tag="VFN42_02470"
     CDS             complement(444..1514)
                     /locus_tag="VFN42_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8995510.1"
                     /translation="MQFGFCVMPNLDDVGFYRHIEELGYQSAWVADSQMLFSDCYALL
                     ALAARETTRLRLGPGTAICGTRIPPVHAAAMMTLNRLAPGRVFLGIGTGNTAMRSMAQ
                     RPMRIAEFDAYLGVLAALLRGETVEYAFNGTPRPIRMLLEGNHAGSLDPKIPLYVSGF
                     GPRAMALAGVHGDGLVFAIPPRGVPVAQALGHVRQGAARANRVIDPAGFHTSALTNLA
                     LLRPGEAADSDRIKAAIGPNVMASVYYFYDEVQERRIDPPPFLARIWKPYCALVEATP
                     PEHRHFRTHEFHYTALHPGEAALIDAQLIRDTCLVGIADELVERIRALEQDGLQELVF
                     AVGTTAKRQIAEDFARQVIARM"
     gene            complement(1560..2426)
                     /locus_tag="VFN42_02475"
     CDS             complement(1560..2426)
                     /locus_tag="VFN42_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET8995511.1"
                     /translation="MAIRKVLLPLTGTAAGEAALSTALMIARVWNAHVTCLHVRVDSR
                     DVAPLAGEGLSGAMIEEMMSATEKESSERAYAVRSMFERFVTRHGVAVEQSPGLSDHA
                     TASFAAVTGREEDLVAQQARLADLTVVPHPDAGEDVSSSDALHAVLFDSGRPVLISPQ
                     VAPTTIGNRVCIAWNGTAESAASVLAALPWMKRAEAVRIFSADGYQRRGPGAPELLQY
                     LALHGVQADIATFQSVGSSVGAGLLAAAAQFDCDLLSMGAYSHSRLRQLILGGVTRHI
                     LEHANLPVMMSR"
     gene            complement(2478..4022)
                     /locus_tag="VFN42_02480"
     CDS             complement(2478..4022)
                     /locus_tag="VFN42_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8995512.1"
                     /translation="MAQASSPFPDPLHPSAAEGASHPQAEAIALLRRRSGTDPVETHI
                     SLVFIGRDTVWKLKKAVRLPFLDFTSLAARAHFIRRELELNRPAAPGLYRDIVPLVRA
                     ADGMLVVGEPEDGRPAEDWVLRMAPVPPADFLDVIAAAGGLDPALQDALADAVAAYHA
                     ALPPVCGVLPPMRQILLGNVDSALAAGLDPAAVQAWRDAELVALDALAPWMRQRAEAG
                     YVRRAHGDLHLGNLCLWQGRPVPFDALEFDEALATIDLAYDLAFLLMDLDHRVSRAAA
                     NRVMNRYVARTGDSDLVRGLPAFLSMRAMVRAHVEARSGHATRVRPYLDAALGYLRPR
                     PALVVAVGGLPGSGKSTLARALAPALGRAPGALVLRSDEIRKRRFGVPPEQRLPAEAY
                     ADAVSQAVFAALAADAGRTAASGHCVVADATFMDPAHRQALEAASRAAGVPFVGLWLQ
                     APMAELERRVIARTGDASDATLAVLRQAAQHDPGAGDWLPLDAANASTALDLARGALH
                     PHSGSC"
     gene            4616..5200
                     /locus_tag="VFN42_02485"
     CDS             4616..5200
                     /locus_tag="VFN42_02485"
                     /inference="COORDINATES: protein motif:HMM:NF018587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory CII family protein"
                     /protein_id="HET8995513.1"
                     /translation="MHNRAAAAEADGFSRLAHRILVQEKVARLQGVATALGMSYANLY
                     ARVTGRVTFKPAEISRLIRAVPDQRLCEFLLRDSDFVAVRRPAAGLPGHTDVLHAATT
                     LAEQALAIIAQTGAALAGGTTRPLAQEQIQHHVAAAEHALCTLRTGLPLLKSAVLLGA
                     KPHRTGMPARTIAISPGDASPPALQQVAARAVAV"
     gene            5841..6473
                     /locus_tag="VFN42_02490"
     CDS             5841..6473
                     /locus_tag="VFN42_02490"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET8995514.1"
                     /translation="MRATTVRHEPGQAHTSHPMRPAMRVKRPIGTAIGLLLCTQAHAG
                     LLGDTLTASYRYPDLAETYNQAIWTPPSFVVGPGAETTASIEDVTQIHVDFTDATLVV
                     TLHTVLSAPAWNASTFSGPVFVTDRPLGIHAIRIDPATTMSGFDASRVWLTDAGIAIN
                     WAGLSYIDGTSVSIDFNFNAPEPPSLGLVGIGLAALAMIRRYALQRRNAP"
     gene            6689..7318
                     /locus_tag="VFN42_02495"
     CDS             6689..7318
                     /locus_tag="VFN42_02495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET8995515.1"
                     /translation="MHARLLATVALSLLTAVPASATVSYSDLSLTIGSAVYAPTEAIP
                     SLDNGNPTRETAILNRAFGTSLSFLAKSDDAHPGSGTYQGIRFTVDTSRGSAGSWTVA
                     WQDINGAQGLNLPVIMDLGVGLFAASGGAGYLFEDVLLPVAPNHGSGVFNISFASHGE
                     WNCADVSHLTLIGGDPRTPSRDVPVPEPTSMVLLGSGLLGLGVLRRLRC"
BASE COUNT         1297 a         2542 c         2528 g         1143 t
ORIGIN      
        1 aggcgatcga ggcgctgcag aagggcggcc agcagatgag ccggaccatg gcgcagcagt
       61 tcggctccgg ccagcaaggc gaggaggccg acggcgagga cggcggtgaa ggccagatgg
      121 gcatgcagga ccagcagggc gagggtgagg gtagcggctc gctctatggc cgccgtggac
      181 ggcaggacgg ccgcggacgc gacccgctgg gccgcgaact gggccagggc agctccggca
      241 ccgacgagag caacgacgtg cagatccccg aaaagcgcga atggcaacgc acacggcaga
      301 tcgaaaacga gctgcgccag cggggagcag agcgtgaccg gccgcagcag gagctggatt
      361 acatcgagcg gctgctgaag cagttctgat tcggacacga atggccgaaa ccgcgccccg
      421 gtcatggccg gccgcacgtc gcgctacatc cgtgcgatca cctggcgcgc gaaatcctcg
      481 gcgatctgcc gcttggcggt ggtgccgaca gcgaagacca gctcctgcaa tccgtcctgt
      541 tcgagagcgc ggatacgctc caccagttcg tcggcgatgc cgaccaggca ggtatcccgg
      601 atcaactgcg cgtcgatcag cgcggcttca ccgggatgca gcgcggtgta gtggaattcg
      661 tgcgtgcgga agtggcggtg ctccggcggc gtggcttcca ccagggcgca gtagggcttc
      721 cagatgcggg ccaggaatgg cggcggatcg atccggcgtt cctgcacctc gtcgtagaag
      781 taatagacgc tggccatcac gttcggaccg atcgccgcct tgatgcggtc ggaatccgct
      841 gcctcgcccg gccgcagcag cgccagattg gtcagcgcgc tggtatggaa gccggccgga
      901 tcgatcacgc gattggcgcg cgccgcgcct tgccgcacat gccccagcgc ctgtgccacc
      961 ggcacgccgc gcggcggaat ggcgaacacc aggccgtcgc cgtgcacgcc cgccagggcc
     1021 atggcgcggg gaccgaatcc cgaaacgtag agcgggattt tgggatccag gctgccggcg
     1081 tgattgcctt ccagcagcat gcggatcggg cggggggtgc cgttgaacgc gtactccacc
     1141 gtttcgcccc gcagcagcgc cgccagcacg cccagatacg cgtcgaattc ggcaatccgc
     1201 atcggacgct gcgccatgct gcgcatcgcg gtattgccgg tgccgatgcc caggaagacc
     1261 cggccgggcg ccaggcgatt cagggtcatc atcgccgccg catgcaccgg cgggatgcgc
     1321 gtgccgcaga tcgccgttcc gggacccagg cgcaggcgcg tggtctcccg cgcggccagt
     1381 gccagcaacg catagcagtc ggagaacagc atctggctgt cggccaccca ggcagactgg
     1441 tagccgagct cctcgatgtg ccggtagaac ccgacatcat cgaggttcgg catgacgcag
     1501 aaaccgaatt gcatgcggtc gctttcccgt cggacggtgc gggtgccggc agcgccggat
     1561 cagcggctca tcatcaccgg caggttggcg tgctccagga tgtgccgggt cacgccgccc
     1621 aggatgagct gtcgcaggcg ggaatgcgaa taagcgccca tcgacagcag gtcgcagtcg
     1681 aactgcgccg cggcggccag caggcccgcg ccgaccgagc tgcccaccga ctggaaggtc
     1741 gcgatatccg cctgcacccc gtgcagtgcc aggtattgca gcagttcggg cgcgccggga
     1801 ccgcgccgct ggtagccatc cgcggagaaa atgcggacgg cctcggcccg tttcatccag
     1861 ggcagtgccg ccaggaccga ggcggccgat tcggcggtgc cgttccaggc gatgcacacg
     1921 cggttgccga tcgtggtcgg cgccacctgc ggcgagatca gcaccgggcg gccggaatcg
     1981 aatagcaccg cgtgcagcgc gtcggaggac gacacgtcct cgccggcatc gggatgcggc
     2041 accacggtca ggtcggccag gcgtgcctgt tgcgcgacca ggtcttcctc ccgcccggtc
     2101 accgcggcga agctggccgt ggcgtggtcg ctcagcccgg gggactgctc gacggcgacg
     2161 ccgtgccggg tgacgaaccg ctcgaacatg gagcggaccg cataggcacg ctcgctgctc
     2221 tccttctcgg tggcggacat catttcttcg atcatcgcac ctgacaggcc ctcgccggcc
     2281 agcggcgcca cgtcgcggct gtccacccgg acatgcaggc aggtgacatg cgcgttccac
     2341 acccgggcaa tcatcagggc ggtggatagc gcggcttctc cggcggcggt gccggtcagc
     2401 ggtagcagaa ccttgcggat cgccatggcg aactccctgg ggatgtaccc cgcactgcgc
     2461 agatcaggtc tgcgtttcta gcacgatcca ctatgggggt gcagtgcgcc gcgtgccaag
     2521 tccaatgccg tactcgcgtt cgccgcgtcg agcggcagcc agtccccggc gccgggatcg
     2581 tgctgcgccg cctggcgcaa cacggcgagc gtcgcatcgg aggcgtcgcc ggtgcgggcg
     2641 atcacccgcc gctccagttc cgccatcggc gcttgcagcc acagcccgac gaagggcacg
     2701 ccggcggcgc ggctcgcggc ctccagcgcc tggcgatgcg ctggatccat gaaggtggcg
     2761 tcggccacca cgcagtgccc ggacgccgcg gttcggccgg catccgcagc cagcgcggcg
     2821 aacaccgcct gggacactgc gtcggcgtag gcctcggcgg gcagccgctg ctccggaggc
     2881 acgccgaagc ggcgcttgcg gatttcgtcg ctgcgcagga ccagggcgcc gggtgccctg
     2941 ccgagtgcag gggccagcgc gcgggccagg gtggatttgc cactgccggg caggccgccg
     3001 accgccacca ccagcgccgg gcgtggtcgc agatagccca aggcagcgtc gagatagggc
     3061 cgcacccggg tggcgtggcc gctgcgcgcc tccacatgcg cacgcaccat ggcccgcatc
     3121 gacaggaagg caggcaggcc gcgcaccagg tcgctatcgc cggtgcgggc gacatagcgg
     3181 ttcatcaccc ggttggcggc ggcgcgcgac acgcggtggt cgagatccat cagcagaaag
     3241 gcgagatcgt aggccaggtc gatcgtcgcc agcgcctcgt cgaattccag tgcgtcgaac
     3301 ggaacgggcc ggccctgcca caggcacaga ttgccgaggt gcaggtcgcc atgcgcgcga
     3361 cggacatagc ccgcctcggc acgctgccgc atccagggcg caagcgcatc cagcgccacc
     3421 agttcggcgt cccgccaggc ctgcacggcc gcggggtcga gccccgccgc cagcgcggag
     3481 tcgacgttgc ccagcaggat ctgccgcatc ggcggcagca cgccgcacac gggcggcagt
     3541 gccgcgtgat aggcagcgac ggcatcggcc agcgcatcct gcagcgccgg gtcgaggccg
     3601 cccgctgcgg cgatcacatc aagaaaatcg gccggcggca ccggcgccat gcgcagcacc
     3661 caatcctcgg cggggcgccc gtcctccggc tcgccgacca ccagcatgcc gtcggccgcg
     3721 cggaccagcg ggacgatgtc gcgatacagg ccgggcgcgg cgggacggtt cagctctagc
     3781 tcccggcgga tgaaatgggc gcgtgcggcc aggctggtga agtcgagaaa cggcaaccgc
     3841 acggccttct tcagtttcca caccgtgtcg cggccgatga acaccaggga gatatgcgtc
     3901 tccaccgggt ccgtgccgga gcgccggcgc agcagggcga tggcctcggc ctgtggatgc
     3961 gatgcgcctt ccgcggctga agggtgaagc ggatctggga agggtgagga agcttgtgcc
     4021 atcgcgccgg tgcccgtccc gccatgatag cgcggaaccg ccgatgctgc ctcaccaaag
     4081 cgtcctggcc tggtgcaggg aaggaactgg aaccacggac gaaccatggc gaacgcggac
     4141 ggcattgcac ggatcgtggc gaagtcgggt gcgccggttc agccgcgggc acagcaaaca
     4201 cgtgccttcg gccggcagtc gtatgggtgc cgtaaattct ggaacaatgc gccaaccggt
     4261 gctaagttcc ctgcttgttc gcaagactgt ggggagcggc gggcgatcac ggcacaaggg
     4321 tgccgtccct cgccagatgg tccagggcgc aaaacgcgac agagttcctg cgtcgctttt
     4381 ctggacgagt tacgcatgca tatcggcggc ggactgtatg cacccagcgc gcaaacgcgc
     4441 gctcaatacg acctgcgaca ggtcaccccg gatcgtttgc caggcggtga cgtgctgcac
     4501 ggcgcgacac catgggcggc aggattgcct ggcaccacct ggctcttcat cgcggatgag
     4561 gcggatcccg ccggcgacca ggttcgctca agcgatcatg tcacaggggg gtgccgtgca
     4621 taatcgtgcc gctgcggccg aggcagacgg cttctcccgc ctggctcacc ggatcctggt
     4681 gcaggaaaag gtcgcgcggc tgcagggtgt cgccacggcg ttggggatga gctacgccaa
     4741 cctctacgct cgggttaccg ggcgcgtgac gttcaagccg gcggagatca gccggctcat
     4801 ccgcgcggtt cccgaccagc ggttatgcga gttcctgctg cgcgacagcg actttgtcgc
     4861 cgtccgacgt ccggcagccg gcctgcccgg tcacaccgac gtgctgcacg cggcgacaac
     4921 gctggccgaa caggcactgg cgatcatcgc gcagaccggc gccgctctgg ccggcggcac
     4981 cacccgcccg ctggctcagg agcagatcca gcaccacgtc gctgccgcgg aacacgcact
     5041 ctgcaccttg cgcaccggcc tgcccctgct gaagtcggcg gtcctgctcg gcgccaagcc
     5101 acaccgcacg ggaatgccgg cacggacgat cgcgatcagc ccgggggatg cctctccgcc
     5161 ggccttgcag caggttgccg cgcgtgcggt tgcggtctga cctcgtccag accgcacggc
     5221 ggatgccgac gcgttcggca ttgccgagtg ccgagccgcg gcaggcgcag gtcccgggcg
     5281 ggatagcacc cgaacagcgt gatggcgccc ggagcggcct gcccgcccag ccgcgccgag
     5341 ggacgccagc gccgttacac aggcggaaga gcagcacgca gccccggcac caggatttcc
     5401 tgctgtaaag catcccgact gcctgggacc agacgtgcga cgcggcgcga gacgcgaaat
     5461 gccgtgcgta tggcttgccg gcactgcacg caccccggcc gctgtcgcgg ctgcgttgct
     5521 ctgcccgccg ggcgcgacgt cgcttcgctt tacagctttc atcgcagacg ctccgcatac
     5581 ctttgtcggc tgccggaatt ttgttggtgc gtttcaatcc gctccggacg tgcgctgcaa
     5641 atattgcacg gaatttttca tcccgcttgc atcggacaat ctgacacacg ctgacaacca
     5701 acagcattgt gggggttcgg cgcggagcgc aacacgccgg acgtgccggc gaatgcggcc
     5761 tgcatcaacg gcggcgcaga acttgcacgt ggtcgcctgc cgaaaggtgc ggccgaaaac
     5821 agccagcacg tgcccgcgac atgcgcgcca cgaccgttcg gcacgagcct ggccaggctc
     5881 acaccagcca tcccatgagg cccgccatgc gcgtcaaacg accgatcggg acagcaatcg
     5941 gattgctgtt atgcacgcag gcccatgcag gtctgctcgg tgatacgctg accgccagct
     6001 accgctatcc ggaccttgcg gaaacctaca atcaggcgat ctggacaccg cccagcttcg
     6061 tggtcggacc gggtgcggaa accaccgcat ccatcgagga cgtgacgcaa atccatgtcg
     6121 acttcaccga tgcgacgctc gtcgtgacat tgcacaccgt tctgtccgcg ccagcctgga
     6181 acgcaagcac cttcagcgga cccgtcttcg tcacggatcg tccgctggga atccatgcga
     6241 tccgcatcga cccggccacc acgatgagcg gcttcgatgc gtcacgcgtc tggctcaccg
     6301 acgccggaat cgccatcaac tgggctggct tgagttacat cgatggcacc agtgtcagca
     6361 tcgacttcaa tttcaatgca ccggaaccac ccagcctcgg gctggtgggt atcggcctgg
     6421 cggctctcgc gatgatcagg cgctacgcac tgcagcgacg caacgcgccc tgaattttcg
     6481 gattttccgt atttgccacg ggcttgggaa tggccaaggc atttctcaca tcggtgcaca
     6541 gccagacaga taacacgcat cagtctgtat tattatctat cgtgcagaac aggctacact
     6601 tcatcggcac gtggtttgca actgaaacgg caagaacgga cgggatggaa ccaaggagtt
     6661 tcccaatccc tgcaaggagg ttcttatcat gcatgcccgt cttttggcga ctgttgcgct
     6721 ctcgctgctc accgccgtac cggccagcgc gacggtcagt tacagtgacc ttagcctgac
     6781 cataggctcg gccgtctatg ctccgaccga ggcaatacca tcgctcgaca atggcaaccc
     6841 cacccgggaa acggcgattc tcaaccgtgc tttcggcacc agcctcagtt tccttgccaa
     6901 atcggatgat gctcatccgg gcagcggcac gtaccagggc atcaggttca cggtcgatac
     6961 aagtcgcggc agcgctggaa gctggacggt ggcgtggcag gacatcaatg gtgcccaggg
     7021 actcaacctg ccggtcatca tggatctcgg cgtcggcctg ttcgccgcga gcggcggcgc
     7081 gggctacctg ttcgaagacg tattgctgcc ggtagccccg aaccacggct cgggtgtgtt
     7141 caacatcagt ttcgcttcgc acggcgaatg gaactgtgcg gatgtctcgc atctgacgct
     7201 gatcggcggc gatccgagga ccccatcccg agacgtgccg gtaccagagc cgacgtcgat
     7261 ggtgctgctg ggcagcggcc ttctcggact gggcgtgctc cggcggcttc gctgctgacc
     7321 acggccggaa ggcggcgtgc tccgctgcct tccggcatcc aaccgccgcc cggcgcgcac
     7381 ctcgcaagga ccggccggaa gactggagcc cgcgcggcca gaacggtccc gcccccgtca
     7441 gaaccgacaa cgctcactga ccccaatcat ggaggtctgg catgccactc atttgctgga
     7501 ccgttgttca
//
LOCUS       DASSZH010000120         9070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_241762, whole genome shotgun sequence.
ACCESSION   DASSZH010000120 DASSZH010000000
VERSION     DASSZH010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 9070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9070
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241762"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="VFN42_02500"
     CDS             complement(<1..811)
                     /locus_tag="VFN42_02500"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET8995516.1"
                     /translation="MRLLQSNVFWTGLEAGTSAGLSFASAFIVARLVGPTEVGVGAAI
                     VAVHVLLWVVVNALFADPLVQRLDVDDRDASSALWASTLVGVVAAFVQAATALPIAHA
                     IGDARIVPMGLLLALPLPLVGAGGVMQGMLTRNREYRVLAGRALIGQGGGTLAGIALA
                     LLGAGAWALVAQQLVNSMFGALSLVLRTPWRPRLVLHWAPVRDLLRIGFPLVLSTLVQ
                     HSRYRLFALLIGGTAGAAALGQVHMAFRLVDTVRELLSTALWRLSLPSMSER"
     gene            990..2063
                     /locus_tag="VFN42_02505"
     CDS             990..2063
                     /locus_tag="VFN42_02505"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET8995517.1"
                     /translation="MSSTSLPAGSVAFARVRPATRLSDLDRAKGLAILLVVFGHLVAR
                     RSPADVGWYDPLRIAVYLFHMPLFMYLSGYVTFLSGAAHTPASGWAHLARRRAVRLLT
                     PFLAFGLAILCGKLIVSHFAAVDNVPPGFWPGVRALVWDTQHSPATEVWYIACLFVFS
                     LATPPLLALDRYRVVLLGVAALLFVLPLPPVLYLDRIGSYFIFFVAGGLAAEAGPSWL
                     RVVDRIWPLALAALALVAMPVAVGWIHFHWTAGAQGFPYKWALLAAGLLSMPAAHGLV
                     RYGAMARAGALALLGRYVFVIYLLNTVFIGLAKAVLLKVVSWDGPHFLPFAAVLMLAG
                     VFGPILTKRWVLQHVPALDRATD"
     gene            complement(2002..3441)
                     /locus_tag="VFN42_02510"
     CDS             complement(2002..3441)
                     /locus_tag="VFN42_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995518.1"
                     /translation="MPVVSVSTDCELRPRAARNVLVSGAAVLLLLLWPALVNRYPLVF
                     SDTGTYVSQIVERHLGWDRPVFYSLMILPLHLTLTLWPVIVAQAALTLCVLKIAMRCF
                     AGSARQLPAILLVLAAGTSLPWVTAQIMPDFTTGLLALLLAMLVIVPERLGLRLALFT
                     AATATALIAMHQANVPLSLMLLVVLLPLRRRLGAAAALGRLGVLLACGPLIAATLALM
                     AVNAVGHGRFSPSPYGNIFVLARSLSDGPAMDALRRHCPRAGWRLCVLVQEGQPRDPD
                     AFLWHADSALYREGGPKVISAEANAILLTALRDEPGAVLWAALRDSVRQFGMVATGDG
                     LDPWPQTVTPVIRRDFPSAELHRYQRAVQTREGLVLPRWLRTIHATLLAFGIVATVLW
                     LLLGLRRRHPIAGLCAATLLCLVGNAAITGALSGPHDRYQARIGWLPVFTPLVAAVAL
                     RRRGDVTAGLNPSPGRVPAHVAAPTAWSG"
     gene            3507..4079
                     /locus_tag="VFN42_02515"
     CDS             3507..4079
                     /locus_tag="VFN42_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003619324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HET8995519.1"
                     /translation="MRFYMRGMLTFSLLLGMLGLAACSGPGNLPPIPQTSEADYHLGP
                     GDQVRIITFGDEQLTGEFRVKDDGTVSMPLLGAVKAAGLTTNQLGNEVSKALIARDLY
                     KNPSVSVEVTAYRPVFVLGEVARPGQYPYQPGMTVQTAVAIAGGFTYRAVEDAFRITR
                     TVNEKAVEGRGEPGTAVRPGDVIKVYERSF"
     gene            4287..5630
                     /locus_tag="VFN42_02520"
     CDS             4287..5630
                     /locus_tag="VFN42_02520"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET8995520.1"
                     /translation="MAAFGAGALLLPLAAQAQMPDRYTLNNYFVSGIPGYGTQLGTTV
                     LSRARPEYDPLGVRVGDFIIRPEVDESVGYNSNIIGQVPAQGSFIEETNASLKFASDW
                     ARNSLGGAFTVTNEQVPGLPSQNTTSWSAALGGTYQIGNDTLTLAASHLNAFIMPYGL
                     DVVSFNKPGLLYFAPIPYSVNDMRVSYQATFGNFSIVPAFDFTNVSFGTTQYFGLNGF
                     VPPPSVNGFVDGQPENLNYLNHNIYTGSITARYEYAPLRDALFVVREANVQYATSDPT
                     LYGPNRSGNAIDMLAGLDYTADAVWRYRALVGYEIRQYANFPTHSSPVFEGNVIWQPS
                     GLDTFTLEFLRTIEDAVDVDIAGFVLTSGRLQWDHELRRNILLTAYGSIQHANYLQTN
                     GGAETFFGTGAGVTYLMNRNVHVALTYDWVDHNGVNGFGPNYLQNVALLQLKLAM"
     gene            5748..6104
                     /locus_tag="VFN42_02525"
     CDS             5748..6104
                     /locus_tag="VFN42_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8995521.1"
                     /translation="MAARKILIVEDEFLIRLTLSEALSDDGFEVIEAGNGEEAIEAMT
                     PDVAVLVTDMQLPGGMDGAEVARRARAAQPALQVIYMTGRPESVAALTTPRDAFIAKP
                     YLPSEVCATVHRLLGS"
     gene            6176..6523
                     /locus_tag="VFN42_02530"
     CDS             6176..6523
                     /locus_tag="VFN42_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995522.1"
                     /translation="MSSSRPRRRWPVVVALTALLGLAGCAYGPAGPYYAYGYGYPYYS
                     AYGAYPYAVYGGAWDGWGLGWPFVGGFWGGGCCYGGGYYGGGYYGGGRYGGGFGGGHW
                     AGQGFGGGGGWRR"
     gene            complement(6631..7557)
                     /locus_tag="VFN42_02535"
     CDS             complement(6631..7557)
                     /locus_tag="VFN42_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET8995523.1"
                     /translation="MARHRIGVIGLGKIAQDQHLPVIAASEVFELVAVCSQRGLGAPG
                     AQAFRDPIEMLNADLALDAVVICTPPQARHAIARAALAAGKHVMLEKPPTATLAELSD
                     LAAEAARRGRVLMTTWHSQYNAAVDAARERLAHETVTRLFIEWKEDVRKWHPGQAWIW
                     RAGGFGVFDPGINALSIATKIMPQPLFIAAADLLTPANADCPIAATLRFAGAGDLRAE
                     FDWRQTGGEVWEIRVGTASGHSFVVSAGGARLAVDGAEVVAAPPQEYQAIYRHFDTLL
                     REGRSHVDARPFELVADAFLLGTRTATEPFHD"
     gene            7692..8237
                     /locus_tag="VFN42_02540"
     CDS             7692..8237
                     /locus_tag="VFN42_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HET8995524.1"
                     /translation="MRAFVASLLMLGLAAVPAAAGQFTLTSPDVKAGGKIADKFVFNS
                     FGCTGQNVSPALSWSGAPAGTKGYVLTVYDPDAPTGSGFWHWVMFDIPATVTSLPEGA
                     GTPGKQPEGAVQGTTDYGAQGYGGPCPPKGDKPHRYIFTLYAVKTDKLDVPPNPTAAV
                     VGFVTHYATLAKASFTAHYGR"
     gene            complement(8225..8755)
                     /gene="nuoB"
                     /locus_tag="VFN42_02545"
     CDS             complement(8225..8755)
                     /gene="nuoB"
                     /locus_tag="VFN42_02545"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HET8995525.1"
                     /translation="MRKTLLQGLRRPLTEPPPRTDDPAVTVLARELNTAAQRLLGRGL
                     AIRVVDAGSCNGCELEIHALNNAFYDLERFGLRFVASPRHADVLLVTGPVTRNMRAAL
                     ERTYQAMPAPKWVVASGACAIDGWVFAGSPQCLGGVGAVLPVDVTIAGCPPTPLDLLR
                     GLLALLRPQAAASQRP"
     gene            complement(8764..>9070)
                     /locus_tag="VFN42_02550"
     CDS             complement(8764..>9070)
                     /locus_tag="VFN42_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase expression protein HypE"
                     /protein_id="HET8995526.1"
                     /translation="RIREISESLGLIEQILGRLPEGPVLVKLADGAGEGMALAEGFRG
                     DVLVWLRVAVRRVQRAHARDPSWFQWPLLEAAIEGDIIADFPLCNKSFNGSYAGHDL"
BASE COUNT         1519 a         3171 c         2943 g         1437 t
ORIGIN      
        1 gccgttccga catcgagggc aggctcagcc gccacagcgc ggtgctgagc aattcgcgta
       61 ccgtgtccac caaccggaat gccatatgta cctgaccgag cgcggcggcg ccggccgtgc
      121 cgccgatcag cagggcgaac aggcgatagc ggctgtgctg caccaatgtg ctcagcacca
      181 gcgggaagcc gatgcgcaga aggtcacgta ccggtgccca gtgcagcacg aggcgcggcc
      241 gccacggcgt gcgcagcacc agcgacaatg caccgaacat cgagttcacc agttgctgcg
      301 cgaccagcgc ccaggccccg gcgccgagca aggcgagcgc gatgccggcc agcgtgccgc
      361 ctccctgccc gatcagggcc cggccggcaa ggacgcggta ctcccggttg cgcgtcagca
      421 tcccctgcat gacgccgccc gcgccgacca gcggcagcgg cagcgcgagc agaaggccca
      481 tcggcacgat gcgcgcatcg ccgatcgcat gcgcgatcgg cagcgccgtc gccgcctgca
      541 cgaaggccgc caccacaccg accagcgtcg aggcccagag cgcgctcgac gcatcgcggt
      601 catcgacatc cagacgctgc accagcggat cggcaaacag cgcgttgacg accacccaca
      661 gcagcacatg caccgccacg atcgccgcgc cgacgccgac ctcggtcgga ccgacgaggc
      721 gtgccacgat gaaggccgac gcaaaggaca ggccggcgga cgtgccggcc tcaaggccgg
      781 tccagaagac attgctctgc agcagccgca tcacgctttc ccgtacgatt ctgacactct
      841 cgcgattgcg aacgcgcaga cgcggcgtgg cgatgcagaa taactgcact gccgtgccgc
      901 atggataaaa aacacgccag caggcggaac gccgacggcc acagcgcgaa cgctgtcagg
      961 ccaccgaagt ttaggagaag atcccgcccg tgtcttccac ctccctgccc gccggatcgg
     1021 tcgcctttgc gcgcgtccga cccgccaccc gtctgtccga tctcgatcgt gccaagggac
     1081 ttgcgatcct gctcgtcgtg ttcggccatc tcgtcgcgcg gcgctcgccc gccgatgtcg
     1141 gctggtacga tccgctgcgc atcgcagtgt atctcttcca catgccgctg ttcatgtatc
     1201 tcagcggcta tgtcacgttt ttgtcaggtg ccgcgcatac cccggcatcc ggctgggcgc
     1261 atcttgcacg ccgtcgcgcg gtgcggctat taacaccgtt cctggccttc ggccttgcga
     1321 ttctctgtgg aaagctgatc gtctcgcact ttgcggcggt cgacaacgtg ccgcctggct
     1381 tctggcccgg cgtgcgcgcg ctggtttggg atacgcagca cagccctgca accgaggtat
     1441 ggtatatcgc gtgcctgttc gtgttcagcc tggccacgcc gccgctgctt gcgctcgacc
     1501 ggtatcgcgt ggtgctgctc ggcgtcgctg ccttgctctt tgtgctgccg ctgccgccgg
     1561 tgctctacct cgaccgcatc ggcagctatt tcattttctt cgtcgcgggc ggccttgctg
     1621 cggaggccgg gccgagttgg ctgcgcgtgg tggatcgcat ctggccgctc gcgctcgcag
     1681 cccttgcctt ggtggcgatg ccggtcgccg tcggctggat ccatttccac tggaccgccg
     1741 gtgcgcaggg ctttccctat aaatgggcat tgctggcggc cgggctgctg tcgatgccgg
     1801 cggcgcacgg actggtgcga tacggcgcga tggcacgcgc cggcgcactg gcgctgctcg
     1861 ggcgctatgt gttcgtgatc tacctcctca ataccgtctt catcggcctg gcgaaggcgg
     1921 tgctgctgaa ggtggtctcg tgggacgggc cgcatttcct gccattcgcg gcagtactca
     1981 tgctggccgg cgtgttcggg cctatcctga ccaagcggtg ggtgctgcaa catgtgccgg
     2041 cactcgaccg ggcgacggat tgagtccggc cgtcacgtcg ccgcggcggc gcagtgccac
     2101 cgcggcgacc agcggcgtga acaccggcag ccagccgatc cgtgcctggt agcggtcatg
     2161 cgggcctgac agggctcccg tgatggcggc gttcccgacc aggcacaaca gggttgccgc
     2221 gcacagcccg gcgatcggat gccgccggcg caagccgagc aggagccaca acaccgtcgc
     2281 gacgatgccg aaggccagca gcgtcgcgtg tatggtccgc agccagcgcg gcaggacaag
     2341 cccttccctt gtctgcaccg cccgctgata acggtgaagt tcagccgacg ggaagtcgcg
     2401 gcggatcacc ggcgtgacgg tctgcggcca cggatcgagg ccgtcgccgg tcgcgaccat
     2461 gccgaactgg cgcacgctgt cgcgcagcgc cgcccacagc accgcccctg gctcgtcgcg
     2521 cagcgcagtg agaaggatcg cattcgcctc ggccgagatg accttcgggc cgccctcgcg
     2581 atacagggcg ctgtcggcat gccacaggaa cgcatcaggg tcgcgcggct gtccttcctg
     2641 caccagcacg cacagccgcc atccggcccg cgggcaatgc cggcgcagcg catccatcgc
     2701 cggcccatcg gacagactgc gtgccaatac aaaaatattt ccgtacggcg agggcgagaa
     2761 gcggccatgc ccgaccgcat tcaccgccat cagcgcaagt gtcgccgcga tcagcggccc
     2821 gcaggccagc agcactccca gccggccgag cgccgccgca gccccgagac gccgccgcaa
     2881 cggcaacagc acgaccagca gcattaacga cagcggtacg ttcgcctgat gcatcgcgat
     2941 cagcgccgtc gcggtcgccg ccgtgaacaa tgccagcctc agcccgagtc gctcggggac
     3001 gatcaccagc atggccagca acagcgcgag cagacccgtc gtgaaatccg gcatgatctg
     3061 cgccgtcacc cagggcagcg aggttcccgc cgccagcacc agcaggatcg ccggcagctg
     3121 gcgcgcgctg ccggcgaagc agcgcatggc gatcttcagc acgcacaacg tcagcgccgc
     3181 ctgcgcgacg atcacgggcc acagcgtcag cgtcaggtgg agcggcagga tcatcaggct
     3241 gtagaatacc ggccgatccc agccgagatg ccgctcgacg atctgcgaga cataggtgcc
     3301 ggtgtcggag aacaccaggg gatagcgatt gaccagcgcc ggccagagca gcagcagcag
     3361 taccgccgca cccgaaacga gcacgttccg cgcggcgcgc ggccgcagtt cacaatcggt
     3421 cgagacagaa acaacgggca tgagcgtctc agtacgttcc gatatgatcc aagcgggtcg
     3481 gggggccatt ggggagtaaa tcatccgtgc gcttttatat gcgtggcatg ctcactttct
     3541 ctttactgct tggcatgctc ggtcttgccg cgtgcagcgg ccccggcaat ctgccgccga
     3601 tcccgcagac cagcgaggcc gattaccacc tcggccccgg ggatcaggtg cgaatcatca
     3661 cattcggcga tgaacaattg accggcgaat tccgcgtgaa ggacgatgga accgtctcga
     3721 tgccgctgct cggcgccgtc aaggcggccg gattaacaac gaatcaactt ggcaacgagg
     3781 tgagcaaggc ccttatcgcc cgtgacctct ataagaaccc gagtgtttcc gtcgaagtca
     3841 ccgcctaccg gcctgttttc gtgctgggcg aagtggcgcg cccggggcag tatccttatc
     3901 agcctggcat gacggtacag acggcggtgg cgatcgccgg cgggttcacc tatcgcgccg
     3961 tcgaggatgc gttcaggatc acacgaaccg tgaacgaaaa ggccgtggaa gggcgtgggg
     4021 agccggggac agcggtgcgg ccgggcgacg tcatcaaagt gtacgaacgc tcgttctgac
     4081 cgacaaaggc gccggtgcaa ccgcgctgca agctgcatta ctcacgaaaa cgcgagtaaa
     4141 cgcaggctag cagggcggca gcggatgaca ttatgctgcc tgtgtgcgca gttgcatcgg
     4201 cgccccctga tgcacccgaa cggttgaggg agagttgcaa gttgcaggtg aaacctggct
     4261 gctggttgcc atgccggttc acgaccatgg ccgcgttcgg cgccggcgcg ctgctgttgc
     4321 cgctggccgc gcaggcgcag atgcccgacc gctacacact caacaactat tttgtttctg
     4381 gcattccggg ttacggcacg cagctcggca ccacggtgct gtcgcgtgcc cggccggaat
     4441 acgacccgct cggcgtgcgc gtcggcgact tcatcatccg tccggaggtc gatgaaagcg
     4501 tcggctacaa cagcaacatc atcggccagg tccctgccca gggcagcttc atcgaggaaa
     4561 ccaacgcgag cctgaaattc gcctccgact gggcgcgcaa cagtctcggc ggcgcgttca
     4621 ccgtcaccaa cgagcaggtc ccgggattac cctctcagaa cacgaccagc tggagcgcgg
     4681 cgctcggcgg aacataccag atcgggaacg acacgctcac cctggccgcc tcgcatctca
     4741 atgccttcat catgccgtat gggcttgatg tggtgagctt caataagcca gggctgctgt
     4801 acttcgcgcc gatcccgtac agcgtgaacg acatgcgcgt cagctatcag gccaccttcg
     4861 gcaacttcag catcgtgccg gccttcgact tcaccaacgt gtccttcggc accacccagt
     4921 atttcggcct caacggcttc gtgccgccgc catcggtgaa cggcttcgtc gacggccagc
     4981 cggagaatct caactacctc aaccacaaca tctataccgg cagcatcacg gcccggtacg
     5041 aatacgcgcc gttgcgcgat gccctgttcg ttgtgcgcga agccaacgtg cagtacgcga
     5101 catccgatcc cacactgtac ggcccgaacc gttccggaaa cgcgatcgac atgctggcgg
     5161 gcctcgacta cacggccgac gccgtctggc gctatcgcgc ccttgttggc tacgaaatcc
     5221 ggcaatatgc gaactttccg acgcattcat cgccggtgtt cgagggcaac gtcatctggc
     5281 agccgagtgg cctggacacg ttcacgctgg aattcctccg cacgatcgag gacgcggtgg
     5341 acgtcgacat cgccggcttc gtgctgacct ccggtcggct gcagtgggac catgagctac
     5401 ggcgcaacat cctgctcacc gcctacggct cgatccagca cgccaattac ctgcagacca
     5461 atggcggcgc ggagacgttc ttcggtaccg gcgccggcgt gacctatctg atgaaccgca
     5521 acgtccacgt tgcgctcacc tatgactggg tcgaccataa cggggtgaac ggcttcggcc
     5581 ccaactacct gcagaacgtc gccctgctgc aactcaagct ggccatgtag ggcgcgcggg
     5641 cgccgacgcc acgcagcgcc ggcggccctg gcctgttcct gatgaccccg ttggggtaat
     5701 ttgtcgcgcg cacggacccg cgtccgtgcg gccttgaaag gacgtgcatg gccgcccgga
     5761 agatcctgat cgtcgaggat gagttcctga tccgcctgac gctgtcggag gcgctgagcg
     5821 atgacggctt cgaggtgatc gaagcgggga acggcgagga ggcgatcgag gccatgacgc
     5881 cggacgtggc cgtgctggtc accgacatgc agcttcccgg cggcatggac ggggcggagg
     5941 tggcgcgccg ggcgcgggct gcacagcccg cccttcaggt gatctacatg accgggcggc
     6001 cggagagcgt cgccgcgctg acaaccccgc gcgatgcgtt catcgccaaa ccctaccttc
     6061 catcggaagt ctgcgcgacc gtgcaccggc tgctcgggag ctaggcacga ggcggggtcg
     6121 atttaccgcg cgccgacccc atgtctctgc cgttctcgag tcgtcggagg gagtcatgtc
     6181 ctcctcgcgt ccgcgacggc gctggcccgt cgtcgtcgcc ctgacggcgc tgctcggcct
     6241 ggccgggtgc gcctacggtc ccgccggtcc gtactacgcc tacgggtacg gctatcccta
     6301 ctattcagcc tatggggcct atccctacgc cgtctatggc ggggcgtggg acggctgggg
     6361 gttgggctgg ccgttcgtcg gcggcttctg gggcggcggt tgctgttacg ggggcggcta
     6421 ttacgggggc ggctattatg gcggcggccg ctacggcggc ggctttggcg gcggccactg
     6481 ggccgggcag ggtttcggcg gcggcggtgg ctggcggcgc tgagccggct cagagcctgt
     6541 gaaagaaatc gaacttgcga aggaatgccc cttgcaagtt tctgatttcg ctagggaccc
     6601 cggtcgcaag gggcattcct tcacaaactc tcagtcgtgg aacggctccg tcgccgtgcg
     6661 ggtgccgagc aggaaggcgt cggccaccag ttcgaacggc cgcgcatcga catggctgcg
     6721 accctcccgc agcagcgtgt cgaaatggcg atagattgcc tggtattcct gcggcggcgc
     6781 ggccaccacc tccgccccgt ccacggcgag ccgcgccccg cccgccgaca cgacgaagct
     6841 gtggccggac gcggtgccca ccctgatctc ccacacctcg ccgccggtct gccgccagtc
     6901 gaactcggcg cgcaggtcgc ccgcgccggc gaagcgcagc gtggccgcaa tcgggcaatc
     6961 ggcgttggcc ggtgtcagca ggtcggccgc ggcgatgaac agcggctgcg gcatgatctt
     7021 ggtcgcgatc gacagcgcgt tgatacccgg gtcgaacacg ccgaacccgc cggcgcgcca
     7081 gatccaggcc tggccgggat gccatttgcg gacatcctcc ttccactcga tgaacagccg
     7141 cgtcaccgtc tcgtgcgcca gacgctcgcg cgccgcatcg accgccgcgt tgtactgcga
     7201 gtgccaggtg gtcatcagca cgcgcccgcg ccgcgccgcc tccgccgcga ggtcggagag
     7261 ttcggcgagc gtcgcggtgg gcggcttttc cagcatcaca tgcttgccgg cggcgagcgc
     7321 cgcgcgggca atggcatggc gcgcctgcgg cggcgtgcag atgaccaccg cgtccagcgc
     7381 caggtcggcg ttcagcatct cgatcggatc gcgaaatgcc tgcgcccccg gcgcgccgag
     7441 gccgcgctgg ctgcagacgg cgaccagctc gaacacctcg ctcgccgcga tcaccggcaa
     7501 atgctggtcc tgcgcgatct tgcccagacc gatcacgcca atgcgatgcc gcgccatctc
     7561 gccctcccgc cgtcgccacg cggcactcta ggacgccacc cggcccgctt ccagccgcac
     7621 ggatggctgg cattccaccg gatcgcgcct atgatccgca gttgtaacgc tgacccctgg
     7681 gaggaaaccg catgcgcgcc ttcgtcgcct cgctcctcat gctcggcctc gcggcagtac
     7741 ccgccgcagc cggccagttc acgctgacca gcccggacgt caaggccggc ggcaagatcg
     7801 ccgacaagtt cgtgttcaac agcttcggct gcaccggcca gaacgtctcc cccgctttgt
     7861 cctggagcgg cgcgcccgcc ggcaccaagg gctacgtgct gaccgtctac gatccggatg
     7921 cgccgaccgg cagcggcttc tggcactggg tgatgtttga catccccgca acggtcacca
     7981 gcctgcccga aggcgccggc acgcccggca agcagccgga gggggcggtg cagggcacca
     8041 ccgactatgg cgcgcagggc tatggcggcc cctgcccgcc gaagggcgac aagccgcacc
     8101 gctatatctt caccctttac gcggtgaaga ccgacaagct ggacgtgccg ccgaacccga
     8161 ccgccgcggt ggtcgggttc gtcacgcact acgccacgct ggccaaggcg agcttcaccg
     8221 cgcactacgg ccgctgagac gccgcggcct gcgggcgcag cagcgcgagc aggccgcgca
     8281 gcagatcgag cggggtcggc gggcagccgg cgatggtgac atcgaccggc agcaccgcgc
     8341 cgaccccgcc gagacactgc gggctgcccg cgaacaccca gccgtcgatg gcacaggcgc
     8401 cggacgcgac cacccatttc ggcgccggca tcgcctggta ggtgcgctcc agcgccgcgc
     8461 gcatgttccg cgtcaccggc cccgtcacca gcagcacgtc ggcatgccgc ggcgaggcga
     8521 cgaagcgcag gccgaaccgc tccaggtcgt agaaggcgtt gttgagcgcg tggatttcca
     8581 gctcgcagcc gttgcaggaa ccggcatcga cgacgcggat cgcaagcccg cgcccgagca
     8641 gccgctgcgc cgccgtgttc agttcgcgcg ccagcaccgt gaccgccggg tcgtcggtgc
     8701 gcggcggtgg ctcggtgagc ggtcgccgca gtccctgcag cagcgtcttt cgcatgtgcg
     8761 cgcctagaga tcgtgccccg cgtaggagcc gttgaaggat ttgttgcaga gcgggaagtc
     8821 ggcgatgata tcgccctcga tcgccgcttc cagcagcggc cactggaacc aggacggatc
     8881 ccgcgcatgc gcccgctgca cgcgccgcac cgcgacgcgc agccagacca gcacgtcgcc
     8941 gcggaacccc tccgccagcg ccatcccctc gcccgcgccg tccgccagct tcaccagaac
     9001 cggcccttcc ggcagccggc cgaggatctg ttcgatcagg ccgaggctct cgctgatctc
     9061 acggatgcgg
//
LOCUS       DASSZH010000121         7841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_582441, whole genome shotgun sequence.
ACCESSION   DASSZH010000121 DASSZH010000000
VERSION     DASSZH010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7841
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582441"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..2204
                     /gene="ftsH"
                     /locus_tag="VFN42_02555"
     CDS             279..2204
                     /gene="ftsH"
                     /locus_tag="VFN42_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET8995527.1"
                     /translation="MSNFGRNLALWVIIALLLVVLFNLFQPGTTHTSATQVAYSDFLS
                     EVNSGHVRDVVIQGHTVSGQLTDGRTFQTYTPTDPTLVSKLTDKGVRVIAKPEDSDVN
                     PLLHYLLSWFPMLLLIGVWVFFMRQMQAGGGRAMGFGKSRARLLTEKQGRVTFEDVAG
                     IDEAKGELQEIVEFLKDPQKFQRLGGKIPKGCLLVGPPGTGKTLLARAIAGEANVPFF
                     TISGSDFVEMFVGVGASRVRDMFEQGKKNAPCIIFIDEIDAVGRHRGAGLGGGNDERE
                     QTLNQMLVEMDGFESNEGVILIAATNRPDVLDPALLRPGRFDRQVVVPNPDVNGREKI
                     LRVHMRKVPLASDVDPKVIARGTPGFSGADLANLVNEAALLAARMGKRVVAMAEFEGA
                     KDKVMMGSERRSMVMSEAEKRMTAYHESGHALCAMHEPECDPVHKATIIPRGRALGLV
                     MSLPEGDRYSKSKSKLLSELTMAMGGRAAEEIIFGPDKVSNGAAGDIKMATDQARRMI
                     TEWGMSDKLGMIAYGDNSQEVFLGHSVTQNKNVSEATAQEIDAEVKDMISKAYAKARR
                     LLTENIEELHRLARGLLEHETLSGDEIRTVLRGEPVIRKVVDEPAPDNRRSSVPTAGR
                     PASQGPSGGPAPQPQPG"
     gene            2411..3232
                     /gene="folP"
                     /locus_tag="VFN42_02560"
     CDS             2411..3232
                     /gene="folP"
                     /locus_tag="VFN42_02560"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET8995528.1"
                     /translation="MTQSWAGLSLDRPRVMGILNVTPDSFSDGGSHADTQTAIQAGLA
                     MHAAGADIIDVGGESTRPGSAPVPPELEQARVVPVIRALASSGAKVSVDTRNAATMQA
                     ALEAGAAIVNDVSALAHDPAAAEVVARHGCPVVLMHMRGTPDTMNAAARYTDIAAEVT
                     AELAGRIEAARRAGIARDAIAIDPGIGFAKFARHSLELLRRLPELRVLGCPILVGVSR
                     KAFIGRLGGEAEPRRRLPGSLAAALFALERGAHILRVHDVTETLQAIRVWQALAG"
     gene            3314..4684
                     /gene="glmM"
                     /locus_tag="VFN42_02565"
     CDS             3314..4684
                     /gene="glmM"
                     /locus_tag="VFN42_02565"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014457598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET8995529.1"
                     /translation="MSKARRLFGTDGVRGTANSDPMTAGMALRLGQAAGLLFMRGDHR
                     HRVVIGKDTRLSGYMLEPALTAGFIGAGMDVTLLGPLPTPAVAMLTRSLRADLGVMIS
                     ASHNPYQDNGIKLFGPDGTKLSDQVENEIERVMDSRLGDRLAAPEALGRATRLDDAAG
                     RYIEAAKASFPRGLRLDDLKIVVDCAHGAAYRVAPTVLWELGATVVPLGVSPDGFNIN
                     KGCGSTHPDYLCAQVVEHSADLGIALDGDADRVLLADERGELIDGDQILALIAASWRE
                     SGRLQGDGIVATVMSNLGLERQLAGLGLSLHRTRVGDRYVAERMREAGINVGGEQSGH
                     VILSDFSTTGDGLLAALQVLAVIVQQGRPASEVCRMFTPLPQRLKNVRFSGFSPLQDD
                     RVQRGIIAAEATLNGAGRLLIRESGTEPLIRVMAEGEDEAAVAQVVDELCALIAEVAR
                     IGEGVG"
     gene            complement(4690..5109)
                     /locus_tag="VFN42_02570"
     CDS             complement(4690..5109)
                     /locus_tag="VFN42_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995530.1"
                     /translation="MPLRGGCHCGNLRVHLRLTRPPAENRIRSCACAFCRAHATRTVS
                     DPDGLFEVWADDWALVEPYRFGSRTADYLVCRRCGVYIGAVCETAAGLRAVSNTNALA
                     ERDAFTQQPARPDHEGESTEARLRRRAANWTPAVLHR"
     gene            complement(5118..5864)
                     /locus_tag="VFN42_02575"
     CDS             complement(5118..5864)
                     /locus_tag="VFN42_02575"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET8995531.1"
                     /translation="MTREDLRQQGRTTAATLFGESPDIPKGFGHLLTEAVYGGVWNRP
                     GLAQHDRLLCSIAALAVFPRLRQLRRMLCAALDHALAAEALREILVQAGLYAGFSAAE
                     ESLALLAEVLDERGIDFPADPPEDVSLDELISRGHALMRALHGDRAQQGYAAPDNPVT
                     GALYPTAIQYGYGEIWSRPGLDRRSRALVAIAGFTALRLPEQAAKFAQSSLNVGLSRS
                     EVVEAVIQTAPYSGFPPALNVLAALSQVLR"
     gene            complement(5950..7116)
                     /locus_tag="VFN42_02580"
     CDS             complement(5950..7116)
                     /locus_tag="VFN42_02580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8995532.1"
                     /translation="MCPSAGKLSAFVVSYNRAAIIGTCLRGLRFADELIVVDKSSTDA
                     TPRIAAGIADRVITVPWSPTVEDTRAFAAAQCSHEWILFLDDDECLSPEAAEFVRRER
                     GLPQADAYAFPLRHYILGEHDERAYYWPEHHVRWFRRGALSFGRTVHAGMTLHTDRVM
                     QVAPDRGVCIHHLSHQDTAQWIDKANRYTSRPDRVRSAHAGEDLFAFAGERLRHWSDR
                     TRDAAPGGYPAAVALLRTLYDLIDRLKVWEEEHGLDGAARFQAVCAMLDAGYAAVPVR
                     TDAVIQAAVPDAGALPDAAEALRQSLQAARAEHDRIAVQLSTARDAAERQARDAVARA
                     DAAESRAAALQAMHQAALLRAEQAECALRMMRQSTFWRATSWPRAAVERLRSRR"
     gene            7273..>7841
                     /locus_tag="VFN42_02585"
     CDS             7273..>7841
                     /locus_tag="VFN42_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HET8995533.1"
                     /translation="MTEAYICDFVRTPIGRYAGSLKDVRTDDLAAHPLKTLMARHPGM
                     DWEAVEDVIMGCANQAGEDNRNVARMALLLAGLPSSIPGATVNRLCGSGLDAVGTAAR
                     AIKTGEAELMIAGGVESMTRAPFVMGKATEAFSRSSEVYDTTIGWRFVNAQMKKVYGT
                     DSMQETGENVAEEYQISRQDQDAFAYRSQ"
BASE COUNT         1278 a         2670 c         2682 g         1211 t
ORIGIN      
        1 gctgcagacg ctgccggcga tcctccggtg caatttgctg gtggcggtcc cgcatctggc
       61 ctatcctgac gcacaggcct gtacccggct tgccgtcggg ttcagtcccc ggcgaccggc
      121 ggcaggggct ccgttcttgc cggcgtgaaa cggcagttca catgccgggg atgcgcaaag
      181 acgtacgcgt ccctatcttg tttgctgtaa tgcgcctgtg gttcatggtt tcctgcccgc
      241 cggcgcgagc gatctgggtc ttggacgagc gaaaatagat gagcaatttc ggccgcaacc
      301 tcgcgctatg ggttatcatt gccctgctgc tcgtggtcct gttcaacctg ttccagccag
      361 ggacgacaca caccagcgcg acgcaggtgg cctattcgga cttcctttcc gaagtgaatt
      421 ccgggcacgt ccgcgacgtg gtgattcagg gacacaccgt gtccggccag ttgaccgacg
      481 gccgcacctt ccagacctac accccgacgg atcccacgct ggtctccaag ctgaccgaca
      541 agggcgtgcg ggtgatcgcc aagccggaag acagcgacgt caatccattg ctgcactacc
      601 tgctgtcctg gttcccgatg ctgttgctga tcggcgtctg ggtgttcttc atgcgccaga
      661 tgcaggcggg cggcggccgg gcgatgggct tcggcaagtc gcgggcgcgt ctgctgaccg
      721 aaaagcaggg ccgcgtcacg tttgaggacg tcgccggcat cgacgaggcc aagggcgaac
      781 tgcaggaaat cgtcgagttc ctcaaggacc cgcagaagtt ccagcgcctg ggcggcaaga
      841 tccccaaagg ctgcctgctg gtcggcccgc ccggcaccgg caagacgctg ctggcccgcg
      901 ccatcgcggg tgaggcgaac gtgccgttct tcaccatctc gggttccgac ttcgtcgaga
      961 tgttcgtcgg cgtcggtgcg tcgcgtgtcc gcgacatgtt cgagcagggc aagaagaacg
     1021 ccccctgcat catcttcatc gacgaaatcg atgcagtcgg ccggcaccgt ggcgccggcc
     1081 tgggcggcgg caacgacgag cgcgagcaga cgctgaacca gatgctggtg gagatggacg
     1141 gcttcgagtc caacgaaggc gtcatcctga tcgctgcgac caaccggccg gacgtgctgg
     1201 acccggcgct gctgcgcccc ggccggttcg accgccaggt ggtggtgccg aacccggacg
     1261 tgaacggccg cgagaagatc ctgcgcgtgc atatgcgcaa ggtgccgctg gcctccgacg
     1321 tcgatccgaa ggtgatcgcc cgcggcacgc ccggcttctc cggcgccgac ctggccaacc
     1381 tggtgaacga ggccgcgctg ctggccgccc gcatgggcaa gcgcgtggtg gcaatggccg
     1441 agttcgaagg cgccaaggac aaggtcatga tgggcagcga gcgccgctcc atggtgatgt
     1501 ccgaggccga gaagcggatg accgcctacc acgaatccgg ccatgcgctg tgcgccatgc
     1561 acgagccgga atgcgacccc gtgcacaagg ccaccatcat tccgcgcggc cgggcgctgg
     1621 gcctggtgat gagcctgccg gagggcgacc gctactccaa gagcaagtcc aagctgttgt
     1681 ccgaactgac catggcaatg ggcggccgag cggcggagga gatcatcttc gggccggaca
     1741 aggtgtccaa cggcgcggcg ggcgatatca agatggccac cgaccaggcg cgccggatga
     1801 tcaccgaatg gggcatgagc gacaaactcg gcatgatcgc ctacggcgac aacagccagg
     1861 aggtgttcct cggccactcg gtgacgcaga acaagaacgt gtccgaagcg acggcgcagg
     1921 agatcgacgc cgaggtcaag gatatgatca gcaaggccta cgccaaggct cgccggctgc
     1981 tgaccgagaa catcgaggaa ttgcatcggc tggcgcgcgg cctgctggag cacgagacgc
     2041 tgagcggcga cgagatccgc accgtgctgc gcggcgagcc ggtgatccgc aaggtggtgg
     2101 acgagccggc gccggacaat cgccggtcct cggtgccgac cgccggccgc ccggcgtcgc
     2161 aaggcccgag cggcgggccg gcaccgcaac cccagcccgg ctgaggcgct cgctttagcc
     2221 cggtcttgag tttggcttcc ctggcgccgg tgcgcgattc agatcgcgta ccggcgttgc
     2281 cttgcggtgg gacgtgttgc gcatcgcgtt cggctgcgac gggtcagtac gggcagtcgg
     2341 cagcccctgc ggagcatatc tgaatgacgg cttggggaat ttgctcccgc ccgccttccg
     2401 gaatcctgcg atgacgcaaa gctgggctgg cctctccctc gaccgcccgc gcgtcatggg
     2461 aatcctgaac gtcacaccgg acagcttcag cgatggcggc agccatgccg acacacaaac
     2521 ggcgatccag gccgggctcg ccatgcacgc cgccggcgcc gacattatcg atgtcggcgg
     2581 cgagagcacc cggcccggct ccgcgcccgt accgccggaa ctggagcagg cgcgcgtggt
     2641 cccggtgatc cgcgccctgg ccagcagcgg cgcgaaggtt tcggtcgata cccgcaacgc
     2701 cgccacgatg caggcggcac tcgaggccgg cgcggcgatc gtcaatgacg tctccgccct
     2761 ggcgcatgat ccggcggcgg ccgaggttgt ggcacgtcac ggctgcccgg tggtgctgat
     2821 gcacatgcgc ggcacgccgg acacgatgaa tgccgcggcg cgctatacgg atatcgccgc
     2881 cgaggtcacc gccgaactcg ccggccgcat cgaggccgct cggcgggccg gaatcgcccg
     2941 ggacgccatc gcgatcgacc ccggcatcgg ctttgccaag tttgctcggc actcgctgga
     3001 acttctccgc cgcctgccgg aacttcgggt gctgggatgc ccgatcctgg tgggcgtgtc
     3061 gcgcaaggcg ttcatcgggc ggctgggcgg agaagcggag ccgcgccgcc ggctgcccgg
     3121 ctcgctggcc gccgccctgt tcgcgctgga acgcggcgcc catatcctgc gtgtgcacga
     3181 cgtcacagaa accctccagg cgattcgggt atggcaggct ttggccggct gagcacaggt
     3241 ttcgcttccc cccgctcgcg ccgtgggtct aaacccgcgc cgcaacaatc ggccaggctg
     3301 acggagacgg accttgagta aagccaggcg cctgttcggt actgacggtg tacgcggcac
     3361 ggccaacagc gatcccatga cggccggaat ggccctgcgc ctcggccagg cggccgggct
     3421 gctgttcatg cgcggcgacc atcggcaccg cgttgtcatc ggcaaggata ccaggctgtc
     3481 gggctacatg ctggaaccgg ccctcaccgc cggcttcatc ggcgccggga tggatgtgac
     3541 gctgctggga ccgctgccga cgcccgccgt cgccatgctc acccgcagcc tgcgcgccga
     3601 cctgggggtg atgatctccg cttcgcacaa tccctaccag gacaacggca tcaagctgtt
     3661 cggcccggac gggaccaaac tgtcggacca ggtcgagaac gagatcgaac gggtcatgga
     3721 cagccgtctt ggcgaccggc tggctgctcc cgaagcgctg ggcagggcca cccgcctgga
     3781 cgatgccgcc ggccgctata tcgaagcggc caaggccagc ttcccgcgcg gcctgcgtct
     3841 ggacgacctg aagatcgtgg tcgattgcgc ccacggcgcc gcgtatcggg tggcgcccac
     3901 cgtgctgtgg gaactcggcg ccaccgtggt gccgctgggc gtgtcgccgg acgggttcaa
     3961 tatcaacaag ggatgcggtt cgacccatcc cgattacctc tgcgcccagg tggtggagca
     4021 cagtgccgat ttgggtatcg cgctggacgg cgatgccgac cgcgtgctgc tggccgacga
     4081 acgcggcgag ctgatcgacg gcgaccagat cctggcactg atcgcggcgt cctggcggga
     4141 atcgggccgg ctgcagggcg acggtatcgt cgccacggtc atgtcgaacc tggggctgga
     4201 acgccagttg gccggcctgg gcctgtccct gcaccgcacc cgggtcggcg atcgctatgt
     4261 ggcggaacgg atgcgcgagg ccggtatcaa tgtcggcggc gagcagtccg gccatgtcat
     4321 cctgtcggac ttctccacca cgggtgacgg gttgctggcg gcgctgcagg tgctggcggt
     4381 gatcgtgcag cagggacggc cggccagcga ggtctgccgc atgttcaccc cgctgccgca
     4441 acggctgaag aacgtgcgtt tcagcgggtt ctcgccgctg caggatgacc gtgtgcaacg
     4501 cggcatcatc gcggcggagg ccacgctgaa cggtgccggg cggctgctga tccgcgaaag
     4561 cggaaccgaa ccgctgatcc gcgtcatggc ggaaggcgag gacgaagcgg cggtcgcgca
     4621 ggtggtggac gaactctgcg cgctgatcgc cgaagtggcg cggatcggcg aaggcgtggg
     4681 ctgatccggt cagcgatgca gcacggccgg agtccagttc gcggcgcggc gcctcagacg
     4741 ggcttcggtt gattccccct cgtgatccgg ccgcgccggc tgttgcgtga aggcgtcgcg
     4801 ctcggccagc gcgttggtgt tgctgacggc gcgcaatccc gcggcggttt cacagaccgc
     4861 gccgatgtac acgccgcagc ggcggcatac gagataatcg gcggtgcgcg atccgaagcg
     4921 atagggttcc accagcgccc aatcgtcggc ccacacctcg aacagcccgt ccggatccga
     4981 caccgtgcgc gtcgcgtgcg cgcggcagaa ggcgcacgcg caggaacgga tgcggttctc
     5041 cgccggcggc cgggtcagcc gcaagtgcac ccgcagattc ccgcaatggc agccgccgcg
     5101 gagcggcatg ccggcgctca tcgcagcacc tggctgagtg ccgccagcac gttcagcgcc
     5161 ggcgggaacc cgctataggg cgcggtctgg atcaccgcct caaccacctc gctgcgcgac
     5221 aacccgacat tcagcgagga ttgcgcgaac ttcgccgcct gctccggcag ccgcagcgcg
     5281 gtgaaaccgg cgatcgccac cagcgcgcgc gagcgccggt ccaatccggg gcgcgaccag
     5341 atttcgccat agccgtattg gatggcggtt ggatacagcg cgccggtcac cggattgtcc
     5401 ggcgcggcgt atccctgctg cgcgcggtcg ccgtgcaatg cccgcatcag ggcgtggccg
     5461 cggctgatca gttcatctaa cgatacgtct tccggtggat cggccggaaa gtcgatgccg
     5521 cgctcgtcca gcacctccgc gagcagggca agcgattcct ccgcggcaga gaacccggca
     5581 tacagcccgg cctgcaccag gatttcgcgc agcgcctcgg cggccagggc atgatccagc
     5641 gcggcgcaga gcatccggcg caactggcgc aggcgcggaa acaccgcaag cgcggcgatg
     5701 ctgcacagca ggcggtcatg ctgcgccaag ccggggcggt tccatacgcc gccatacacc
     5761 gcctcggtca gcagatgacc aaatcctttg gggatatcgg gagattcgcc gaacagcgtc
     5821 gctgccgtcg tgcggccctg ctgccgcaaa tcttcgcgcg tcatgtcgtc ctcccatgtc
     5881 ccgtgcatcc tggcgcaccg gtgcagcgcc agggcatccg cgccggtttc gcggcgccat
     5941 gcaagccggc tatcgccggg agcgcaaccg ctccaccgcg gcacgcggcc aggacgtcgc
     6001 gcgccagaac gtgctttgcc gcatcatccg cagtgcgcac tccgcctgtt cggcgcgcag
     6061 cagcgcggcc tggtgcatcg cctgcaaggc ggcggctctg ctctccgcgg catccgcgcg
     6121 ggccaccgca tcgcgcgcct ggcgttcggc cgcatcacgc gccgtgctca gttgcaccgc
     6181 gatccggtcg tgctccgccc tggcagcctg caggctttgc cgcaatgcct cggccgcatc
     6241 gggcagtgcg ccggcgtccg gcaccgccgc ctgtatgacc gcgtccgtgc gtacgggcac
     6301 tgccgcgtag ccggcgtcca gcatcgcaca gacagcctga aagcgggcgg cgccgtccag
     6361 cccgtgttcc tcctcccaga ccttcagccg gtcgatcaga tcgtacagcg tccgaagcag
     6421 tgctacggcc gccggatagc ctccaggcgc cgcatcgcgc gtgcgatccg accaatgccg
     6481 caggcgttcc ccggcgaacg cgaacagatc ctccccggcg tgggccgagc ggacgcgatc
     6541 cgggcgggag gtgtagcggt tggccttgtc gatccactgc gccgtgtcct ggtgcgacag
     6601 gtgatggatg cagacgccgc gatccggcgc cacctgcatc acccggtcgg tgtgcagcgt
     6661 catgcctgcg tgcacggtgc ggccgaagct cagcgcgcca cggcggaacc agcgcacatg
     6721 atgttccggc cagtaatacg cgcgctcgtc gtgctcccca aggatgtagt ggcgcaacgg
     6781 aaaggcataa gcgtctgcct ggggcaatcc gcgttcgcgc cggacgaact cggccgcttc
     6841 cggactgagg cattcgtcgt cgtccaggaa caggatccac tcgtggctgc actgcgccgc
     6901 ggcgaaggcg cgggtgtcct ccaccgtggg cgaccagggg acggtgatca cccggtcggc
     6961 aatgccggct gcgatgcgcg gcgtggcatc ggtggaggac ttgtccacca cgatcagttc
     7021 gtccgcgaag cgcagcccgc gcaggcaagt gccgatgatc gccgcccggt tgtacgatac
     7081 gacgaaggcg ctgagtttcc cggcggacgg acacatcgtt cgtttcgatc gctggcggtg
     7141 cggcggcagc atgcacgggc atgtatgaac ggccggtaaa cgtccgccgc cggcccccag
     7201 ccttcccgcc gcgcgccccg cgcgctacac caccgggcag ccgcgtgccc agcaaacccg
     7261 ggagaaacgc tcatgaccga ggcctatatc tgcgacttcg tgcgcactcc gatcggccgc
     7321 tatgccggct ccttgaagga cgtgcgcacc gacgacctgg ccgcccatcc gctaaagacg
     7381 ctgatggccc gtcatccggg catggactgg gaggcggtgg aagatgtgat catgggctgc
     7441 gccaaccagg ccggcgagga caaccgcaac gtcgcccgca tggccctgct gctggccgga
     7501 ctgcccagct ccattcccgg cgccacggtg aatcggctgt gcggctcggg cctggacgcg
     7561 gtcggcaccg ctgcccgcgc catcaagacc ggcgaggccg aactgatgat cgccggcggc
     7621 gtggaaagca tgacccgcgc ccccttcgtc atgggcaagg ccaccgaagc gttctcgcgc
     7681 tcctcggagg tctacgacac caccatcggc tggcgcttcg tcaacgcgca gatgaagaag
     7741 gtctacggca cggattccat gcaggagacc ggcgagaacg tggcggagga gtaccagatc
     7801 tcccgccagg accaggacgc gttcgcctat cgcagccagc a
//
LOCUS       DASSZH010000122         2772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1605462, whole genome shotgun sequence.
ACCESSION   DASSZH010000122 DASSZH010000000
VERSION     DASSZH010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1605462"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..1382
                     /locus_tag="VFN42_02590"
     CDS             162..1382
                     /locus_tag="VFN42_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET8995534.1"
                     /translation="MHRRGMLAGLGLAVLGGSAARAAENGSPWLSPMQPSGTRAGAKL
                     VRLPGKQPLIQLSERPPNYEAPIETFRNAVTPNDEFFVRYHLAGIPDMDGLAKWSLSI
                     GGDAAQRSLHFASLAELRNGFPEHEVTAVCQCSGNRRGLSDPHVAGVEWGYGAMGSAV
                     WRGVRLKDVLAKAGIKSGAVEIWIHGMDGPVLPTTPAFHKSLPLDKATTDEVLIAYAM
                     NGTALPHYNGYPVRLVVPGWTATYWMKHLSAIEISSKPQPSFWMQKAYRVPAGMFPVD
                     TAFASQNDAKSWPITEMVVNSVIADPIDGAQVPAAGFTVHGMAWDRGHGIRSVEISID
                     GGRTWHLARLDKEHGHFAFRAFSFSTGKLPPGPCTIAARATSKAGETQAEKLKWNPAG
                     YHNNVPQTLTVTVA"
     gene            1394..1687
                     /locus_tag="VFN42_02595"
     CDS             1394..1687
                     /locus_tag="VFN42_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995535.1"
                     /translation="MMRGTLLLAAALVAGPAAADQAPGWMKPGPGQEATAITCGICHS
                     PEYIRMNSVFLTQSQWQAEVGKMREVFGAPMDDDTAKQIVEYLGKNYAAPAAR"
     gene            1806..2264
                     /locus_tag="VFN42_02600"
     CDS             1806..2264
                     /locus_tag="VFN42_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HET8995536.1"
                     /translation="MSPLKSAAIPLATLLLLSGCVSQPTGPMVQVMPAPGKPFEVFQQ
                     DQSVCRYYADQQTAGTAQGANNQQIVTGVIGTALGAGLGAAIGGGQGAAIGAGSGALA
                     GTLVGAGPAQQAQMTLQQRYDQAYVQCMYARGNQVPGYQSPYMPPPQPYR"
     gene            2398..2691
                     /locus_tag="VFN42_02605"
     CDS             2398..2691
                     /locus_tag="VFN42_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995537.1"
                     /translation="MRLAPTILALAAVAMLAGCGTREPNRTAGGAATGAATGAAVGAI
                     GGPPGMAAGAAIGAAAGAGTGAATSPRSVNLGPPPWQPGAPSVGENIARDVKK"
BASE COUNT          482 a          921 c          977 g          392 t
ORIGIN      
        1 atgcgcgctt ccccagcaaa ttcgccggca tgctagcgga ccaggcgatg ccgtcgaggc
       61 ccagccgggc ggtggccggg cgagaaatct tcttgcaacg tattttaaaa ttcggcaaaa
      121 ttatcgtagg acaagaacga gccagaggga gaaattccat catgcaccga cgcggcatgc
      181 tggccggcct gggtttggcc gtgctgggcg gctcggcggc acgcgccgcc gagaacggat
      241 cgccatggct cagcccgatg cagccatcgg gcacccgggc cggcgcaaag ctggtccggc
      301 tgcccggcaa gcagccactg atccagctct ccgagcggcc gccgaactat gaggcgccga
      361 tcgagacgtt ccgcaacgcc gtcaccccca atgacgagtt cttcgtccgc tatcacctgg
      421 ccggcatccc cgacatggac ggtctggcca agtggtcgct ctcgatcggc ggcgacgcgg
      481 cgcagcgcag tctgcatttc gccagccttg cggagctgcg gaacggcttc cccgagcatg
      541 aggtgacggc ggtctgccag tgctccggca atcgccgtgg cctgtccgat ccgcatgtcg
      601 cgggggtgga atggggctat ggcgccatgg gcagcgccgt ctggcgcggg gtgcggctga
      661 aggacgtgct ggccaaggcc gggatcaagt ccggcgcggt ggagatctgg atccacggca
      721 tggatggacc ggtgctgccg accaccccgg ccttccacaa gagcctgccg ctggacaagg
      781 cgaccaccga cgaggtgctg atcgcctatg cgatgaacgg cacggcgctg ccgcactaca
      841 acggctatcc cgtccggctg gtggtgcccg gctggaccgc cacctattgg atgaagcacc
      901 tcagcgccat cgagatcagc agcaagccgc agccgagctt ctggatgcag aaggcgtacc
      961 gggtgccggc ggggatgttc ccggtagata cggccttcgc cagccagaac gacgccaaga
     1021 gctggccgat caccgagatg gtggtgaaca gcgtgatcgc cgacccgatt gacggcgcgc
     1081 aggtcccggc ggccgggttc accgtgcacg ggatggcgtg ggatcgcggc catgggatcc
     1141 ggtcggtcga gatctcgatc gacggcggcc gcacctggca cctcgctcgg ctggacaagg
     1201 agcacgggca cttcgcgttc cgcgccttca gcttcagcac cggcaagctg ccgcccggcc
     1261 cctgcacgat cgcggcgcgc gccaccagca aggcggggga gacccaggcg gagaaactga
     1321 aatggaaccc ggccggctac cacaacaacg tgccgcagac gctgacggtg accgtggcat
     1381 gaggaacgca atcatgatgc gaggcacgct gctgcttgcc gcggccctgg ttgccggccc
     1441 cgccgccgcg gatcaggcgc cggggtggat gaagcccggg ccgggccagg aggccaccgc
     1501 gatcacctgc ggcatctgcc acagcccgga gtacatccgg atgaactcgg tattcctcac
     1561 gcagagccag tggcaggcgg aagtcggcaa gatgcgcgag gtcttcggtg cgccgatgga
     1621 cgacgacacg gcgaagcaga tcgtcgagta cctgggaaag aactacgcgg caccggcggc
     1681 gcgctgaacc ggcagcgcgc catgatattg actcagatca aagaattcca ggtgatctcg
     1741 aattaggcag gggaaggccg gcgcggcgct gttgccccgc cggcaacgat ccgaggaccc
     1801 ttgccatgtc accgctgaag tccgcggcca ttccgctggc cacgctgctg ctgctctccg
     1861 gttgcgtgtc gcaaccgacg ggaccgatgg tgcaggtgat gccggcaccc ggcaagcctt
     1921 tcgaggtgtt ccagcaggac cagagcgtct gcaggtacta cgccgaccag cagaccgccg
     1981 ggaccgcgca gggggccaac aaccagcaga tcgtcaccgg ggtgatcggc accgcgctgg
     2041 gcgccgggct gggcgcggca atcggcggcg gccaaggcgc agcgatcggc gccgggtcgg
     2101 gcgcgctggc cggcacgctg gtgggggccg ggccggccca gcaggcgcag atgacgctgc
     2161 agcagcgcta cgaccaagcc tatgtgcagt gcatgtatgc gcggggcaac caggtcccag
     2221 gttatcaatc gccctatatg ccgccgccgc agccctatcg ctgaatggcg gcgaaccggg
     2281 ggctggatcg ggcaagcggc cgtgaccggc cgcgcgctgt ttgttgaaat tgttcaagcg
     2341 acgagtcggt ccgtttcgcg ttgtgatccc gtgcctaccg caaggaaggg atcaatcatg
     2401 cgacttgccc ccaccattct cgccttggcc gccgtggcaa tgcttgccgg ctgcggaacc
     2461 cgagagccaa accgcacggc tggcggagcg gcgaccggtg ccgcgaccgg agccgcggtc
     2521 ggcgcaattg gcggaccgcc cggcatggcg gccggcgcgg cgatcggcgc ggcggccggc
     2581 gcgggcaccg gtgccgcgac cagcccgcgc agcgtgaacc tcggaccgcc gccatggcag
     2641 ccgggcgccc cgagcgtcgg cgagaacatc gcgcgcgacg tgaagaagta agctatcgcg
     2701 ttcggcgggc gccctgcgca tgcagcggcg cccgccttgc atccagcccg gcggacggcc
     2761 ggctttcgcc gg
//
LOCUS       DASSZH010000123         3073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_991817, whole genome shotgun sequence.
ACCESSION   DASSZH010000123 DASSZH010000000
VERSION     DASSZH010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991817"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            300..473
                     /locus_tag="VFN42_02610"
                     /pseudo
     CDS             300..473
                     /locus_tag="VFN42_02610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
     gene            complement(548..1555)
                     /locus_tag="VFN42_02615"
     CDS             complement(548..1555)
                     /locus_tag="VFN42_02615"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET8995538.1"
                     /translation="MPTEDAIIARRDGRAGRILLNRPAALNALDLDMIRSFTRALEQF
                     RDDPHVHVVVVEGAGDRAFCAGGDIRALRDNQLKGDRGPVEAFFTEEYALNRMIAAYP
                     KPYAALIDGICMGGGIGVSVHGAYRVASEHAMFAMPETAIGFFPDIGATYFLPRLPGR
                     IGIYLGLTGARVRGADAVHAGFATHYTPRERLPALSQALAQDGATALALFAAPLPAFS
                     LAGQRDAIDRCFGVDSVPEIVHRLEADGSEWAQQALGQLRGVSPAALCFTLEALRRGA
                     DLTLPQALDAEFLLTRTTMAYPDFAEGVRAMVVDKDRKPRWQPERIEQVDPRVIAAMF
                     A"
     gene            complement(1623..>3073)
                     /locus_tag="VFN42_02620"
     CDS             complement(1623..>3073)
                     /locus_tag="VFN42_02620"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET8995539.1"
                     /translation="MGSPDWGNDTRFLRKADRVANWDALHAFMSGWSVQHARQWIADA
                     AQQAHVPSFPLREPDELFGSAQLRHRRFFRSVAIDGRALQVPGSPFGLSFAEVDAIPS
                     VETGPMPLSGVRVLDFSWVIAGPTTTRYLAAMGAEVIKVEAPGRGDPGRASELHTVLG
                     QAKRAIVLDLKRPDGVALARALAGKCDVLIENFATGVMDRLGLGADALRGANPNLVYV
                     SASGLGRSGPESHAVAYGTLLQCYSGFAGLNRHPDRPPRVGFAWLDPMCGLMLAFAVA
                     AALWRRQAGRVARIDFSMVEAMLWTMADPLLAAQRTGAPQAMGNASDCHAPHGAWRCA
                     DPDSWLALAVRGDAEWRALCALVPELRGMADLSLGQRRDAQQQIDAALAAWLAPQDAT
                     RMAEQLCSAGIPAAALADSAALVGSAHLRARGFWQVHAAGVLPGLPWTASFGRVNGPA
                     PELGADTDAVLRDILQLSEPELAELRRGGTLG"
BASE COUNT          535 a         1106 c          979 g          453 t
ORIGIN      
        1 gaccggtgcc gccgctgaag cccaagccgc tgctcgctgc cgtgctgctg accggcttcg
       61 cgttccacgg tctgcacatg ccggccgaag cggaaatccg cttcgccgcg cgcggcgcac
      121 gacacctgtt tgacgtctgc cgcccggccg gtccgcttcc cgtccgagtg ccgcgctgat
      181 cccacaatca agttgcacgc ccccggccac gctggcaggc tgcccggcgc aattctatgc
      241 agaaaggcgc cagcatgcag atcgagatcg aatactgcgg acagtgaggc tacgaaccgc
      301 gcgcccgtga ggtgcgggac ctgatcctga agacgcaacc cgctgcaaaa gtccggctca
      361 ccaagagcag cggcggcgtg ttcgagatca cggtggatgg ccggctcgcc tactcgaagc
      421 gagccatcgg ccggttcccg atgaccgagg aagtgctcga cgcactcggt taagcgctcg
      481 catcacgacg aacgcgctgc ctgccgcgtg ccggtggcat cctgtttttt cgcttacccg
      541 gcgatgttca ggcgaacatc gccgcgatga cccgtggatc gacttgctcg atccgctccg
      601 gctgccaacg cggcttgcgg tccttgtcca ccaccatcgc gcgcacccct tccgcgaaat
      661 ccggataagc catggtggtg cgggtcagca ggaattcggc atccagcgcc tgcggcagcg
      721 tcaggtcggc gccacggcgc agtgcttcca gcgtgaaaca cagggcggcc ggcgacacgc
      781 cgcgaagctg gccaagcgcc tgctgcgccc attcgctgcc atcggcttcc agtcggtgca
      841 cgatctccgg cacgctgtcg acgccgaaac agcggtcgat cgcatcgcgc tgcccggcca
      901 gcgagaatgc cggtagtggt gcggcgaaca gcgccagcgc ggtggcacca tcctgcgcca
      961 acgcctgcga caacgccggc agccgctcac gcggtgtgta gtgcgtggcg aacccggcat
     1021 gcaccgcatc ggcgccgcgc acccgtgcac cggtcaggcc gagatagatg ccgatgcgtc
     1081 ccggcaggcg cggcaggaag tacgtggcgc cgatgtccgg aaagaagccg atcgccgttt
     1141 ccggcatggc gaacatcgcg tgctccgacg ccacgcggta cgcgccatgc accgacacgc
     1201 cgatgccgcc gcccatgcaa ataccgtcga tcaacgcggc gtaaggcttc ggatacgcag
     1261 caatcatgcg attcagcgca tattcttcgg tgaagaacgc ctcaaccggg ccgcgatcgc
     1321 ccttcaactg attgtcgcgc agcgcgcgga tatcgccgcc ggcacagaag gcgcgatcgc
     1381 cggcaccttc caccacaacc acgtgcacgt ggggatcgtc gcggaactgc tccagcgcac
     1441 gggtgaagct gcggatcatg tccagatcca gcgcattcag ggcagccgga cggttcagca
     1501 ggatgcgtcc cgcgcggccg tcgcggcggg cgatgatggc gtcttcggtc ggcatgtcgg
     1561 gtctccggtg atcccggttc gtctatccgg ggtagcggcg tggggcaatc cggggcgggc
     1621 cttcagccga gggttccgcc gcgccgcagt tcggcgagct ccggttccga tagctgcagg
     1681 atatcgcgca acaccgcgtc ggtgtccgct cccagttccg gcgccggtcc attgacgcgg
     1741 ccaaaactgg cggtccaggg aaggccgggc agcacgccag cggcgtgcac ctgccagaat
     1801 ccgcgagcgc gcagatgtgc gctgccaacc agcgcggctg aatccgccag cgccgcggcg
     1861 ggaattcctg ccgagcacaa ctgctccgcc atccgcgttg catcctgcgg cgccagccac
     1921 gctgccaggg ccgcgtcgat ctgctgttgc gcgtcccgcc gctgcccgag cgacaggtca
     1981 gccatgccgc gcaattccgg caccagcgcg cacagggcgc gccattccgc atcaccgcgc
     2041 accgccagcg ccaaccagga atcggggtct gcacaacgcc aggcgccatg cggcgcatgg
     2101 cagtcggatg cattgcccat cgcttgcggc gcgccggtcc gctgcgcggc caacagtgga
     2161 tcggccatgg tccacagcat cgcttcgacc atggagaaat cgatccgcgc cacccggccg
     2221 gcctgacggc gccacagcgc cgccgccacg gcgaaggcca gcatcaagcc gcacatcgga
     2281 tccagccagg caaagcccac gcgcggcggc cgatccggat gccggttcaa tccggcgaat
     2341 ccggaatagc attgcagcag cgtgccatag gccaccgcgt gcgattccgg tccgctgcgt
     2401 cccaatccgg atgccgatac atacaccagg ttgggattgg cgccccgcag cgcgtccgca
     2461 cccaggccga ggcgatccat caccccggtg gcgaagttct cgatcagcac atcgcatttt
     2521 cccgctagtg cacgtgccag cgcgacgccg tccggccgct tcagatcgag cacgatcgcg
     2581 cgcttcgcct ggccaagcac cgtgtgcagc tccgacgcgc ggcccggatc gccgcggcca
     2641 ggggcctcca ccttgatcac ctccgccccc atcgccgcca gatagcgggt ggtggtcggc
     2701 ccggcgatga cccaactgaa atccagcacg cgcacgccgg acagcggcat ggggccagtc
     2761 tcgaccgacg gtatcgcatc aacttcggcg aaactcagcc cgaacggtga accgggcacc
     2821 tgcagcgcgc ggccatcgat tgccacagag cggaagaagc gccggtgccg caactgcgcc
     2881 gatccgaaca gttcgtccgg ttcgcgcagc gggaagctgg gcacatgcgc ctgctgcgcg
     2941 gcgtcggcga tccactgcct ggcatgctgc acactccagc ccgacatgaa cgcatgcaat
     3001 gcatcccaat tcgcgacgcg atcggcctta cggaggaaac gcgtatcgtt gccccagtcc
     3061 ggcgagccca tca
//
LOCUS       DASSZH010000124         4432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_651043, whole genome shotgun sequence.
ACCESSION   DASSZH010000124 DASSZH010000000
VERSION     DASSZH010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651043"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..366)
                     /locus_tag="VFN42_02625"
     CDS             complement(49..366)
                     /locus_tag="VFN42_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HET8995540.1"
                     /translation="MAEYRVNGPPAWQPSHPGELLREDILPALRLTVTAAAEKLGVSR
                     QTLHAILSERSAVTPEMAVRLGKLCGDGPDIWLRMQMAHDLWQAQRNLADVVAQIPTL
                     HTA"
     gene            402..693
                     /gene="ssrA"
                     /locus_tag="VFN42_02630"
     tmRNA           402..693
                     /gene="ssrA"
                     /locus_tag="VFN42_02630"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
     gene            complement(707..1945)
                     /locus_tag="VFN42_02635"
     CDS             complement(707..1945)
                     /locus_tag="VFN42_02635"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HET8995541.1"
                     /translation="MLENNAMLTRRILLGSTMTLAAVPAVAQRRHPTRGPAAAAHGNQ
                     PDPSQPPPEPAQTPIGQVATDARWACIVDFNTGTTLLEKNADEQMTPSSLTKLMTLYI
                     TFQRLAEGRLKLTDQLLVSKTAWRFGGSKMFVRVGDTVSVEDLLKGIIVDSGNDACIV
                     LAESISGSQEQFAGLMNETAKKLGMTHSHFMNPMGWPVPDHYMSARDIATLASDLIRS
                     FPQYYHYFGDKTFTYSNITQDNRNELVMRGTADGLKTGHTEEGGFGLCASAERDGRRV
                     VIVLNGMPSSRARAEQGERLMAWSFANFEDVKLYAAGEAVDQAPVWLGASPSVPLVGL
                     RDLVVTVPHGWRRQAKLQISYAAPIRAPVAKGTKLGVLSLAGQGVPSMQLPLVAGADV
                     PKLGLPGRAMAVLTHYVTGG"
     gene            complement(1956..2849)
                     /locus_tag="VFN42_02640"
     CDS             complement(1956..2849)
                     /locus_tag="VFN42_02640"
                     /inference="COORDINATES: protein motif:HMM:NF015298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlpA-like double-psi beta-barrel
                     domain-containing protein"
                     /protein_id="HET8995542.1"
                     /translation="MITRTSRFAAALGACALALLLASCGRQAPPAHPHYVLGQPYQSG
                     GVWWYPREQPRLDATGLAVIYAGGHQALTTDGEVFSQDALAVAHPTLPLPAIARLTDL
                     QTGRSLLVRINDRGTPRPNRLVQVTRRVAALLGMPRDGVAEVRLTLLPGQTASVQDAV
                     GGAPRLSIAAAPVGRVQAAALPPPSGVRQEAGRQAVLPAAPAAALPAESVRLDGTVTQ
                     ESPRPGRLWVRLDTFQSYQYAAMQRAKLVGLAPRIESVFDNREQSFRVMLGPFTDVAA
                     ADSALDRAIAAGVSDARIVVE"
     gene            complement(2910..3689)
                     /gene="otnI"
                     /locus_tag="VFN42_02645"
     CDS             complement(2910..3689)
                     /gene="otnI"
                     /locus_tag="VFN42_02645"
                     /EC_number="5.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HET8995543.1"
                     /translation="MPRFAANLSMMFNEVPFLDRFEAARKADFDGVEYLFPYDFPAAE
                     IRRRLSGAGLTQVLFNMAPGDWAKGDRGLASLPGRQTEFRESVKRALEYAHALDCKLL
                     HCMAGIPAAGVTPVTAAAVYAANLAWAAEQAAPAGVKIVIEPINHRDMPGYHLNTQAQ
                     GAAIVEALGPDRVGLQFDVYHVQVTEGDITKRMEQYMPVIAHMQIADVPARNEPGTGE
                     IGWSYVFRRMDELGYQGWVGCEYRPAGETVAGLRWRQQFGA"
     gene            complement(3774..>4432)
                     /locus_tag="VFN42_02650"
     CDS             complement(3774..>4432)
                     /locus_tag="VFN42_02650"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine dehydratase"
                     /protein_id="HET8995544.1"
                     /translation="VPQGNSVEKNAAMRAFGARLIEHGTDFEMARDEAMRLAAAEGLE
                     AVPSFAPDLVKGVATWSLELFRGTPPLDALYVPIGLGSGICGAILTRDLLGLGTEIIG
                     VQAAGADCYARSFAEGRVVSLNRADTLADGVAVRQPDPRALAIIRAGAARIVTVTDAE
                     IGGAIRAYWTDTHNLVEGAGAVPLAALLQERERMQGRRVGLVVSGGNIDLALFRQWVG
                     "
BASE COUNT          718 a         1556 c         1446 g          712 t
ORIGIN      
        1 ctgctgctgt gctgtcaagg tcatctgggg agccccggcg ggttcgtgtc aggctgtatg
       61 cagcgtggga atttgtgcga cgacgtcggc gaggttgcgc tgcgcctgcc acagatcgtg
      121 cgccatctgc atgcgcagcc agatatccgg cccatcgccg cacagcttgc ccagccgaac
      181 tgccatttcc ggcgtcaccg ccgagcgctc ggagaggatc gcatgcagtg tctggcggga
      241 cacccccaac ttctcagccg ccgccgtgac ggtcagccgc agggccggca aaatatcttc
      301 gcgcagcaac tctccaggat ggctgggttg ccaagcaggc ggtccgttca cccgatattc
      361 agccatgacc cgtaatcctt gctcacacga agcagcacgc gggccttttc cccgcccgcc
      421 cggcgcccta tatgaaccct cgctggttcg ccggctatgg cgataaacgg ggtgcgaaat
      481 aagcgttgtg gacccggggg cagtgcccgg cgcctccacc attcatgggg gcgaaacagg
      541 ctcgacacgc gtggtaaagg cacacttttc gctcggcatg gttccgccgt catcgggcca
      601 ttctacaagt gccaatgaca atgaggcgct cgctatcgct gcataagcga taagcgcggt
      661 tcggggggca ccgggcaaca gaagcccccc acttcatccc cgcagatcag ccacccgtca
      721 catagtgcgt cagcaccgcc atggcgcggc ccggcagccc cagtttcggc acatcggcgc
      781 cggcgaccag aggcagttgc atcgacggga cgccttgccc ggccagactc agcacgccaa
      841 gcttggttcc cttcgccacc ggcgcgcgga tcggcgcggc atagctgatc tgcagctttg
      901 cctgccggcg ccagccgtgc ggcactgtca ccaccaagtc gcgcagaccc accagaggca
      961 cgcttgggct ggcgccaagc cagaccggtg cctggtccac cgcctccccg gccgcgtaca
     1021 gcttcacgtc ctcgaaattg gcgaaggacc acgccatcag ccgctcaccc tgctcggcgc
     1081 gcgcccggct gctgggcatg ccgttcagca cgatcacgac gcgccggccg tcacgttccg
     1141 cgctggcgca caggccaaag ccgccttcct cggtatgccc ggtcttcaat ccgtccgcgg
     1201 tgccgcgcat caccagttcg ttgcggttgt cctgggtgat gttggagtag gtgaacgtct
     1261 tgtcgccgaa gtagtggtag tactgcggaa agctgcggat caggtccgac gccagcgtgg
     1321 cgatgtcgcg cgcgctcatg tagtggtccg gaaccggcca ccccatcgga ttcatgaaat
     1381 gcgaatgggt catgccgagc ttcttcgccg tctcgttcat cagaccggcg aattgctcct
     1441 gtgacccgga aatcgattcc gccagcacga tgcacgcatc attgccggaa tccacgatga
     1501 tgcctttgag cagatcctcc acgctgaccg tgtcgcccac ccgcacgaac atcttggaac
     1561 cgccgaaccg ccatgccgtc ttgctgacca gcaactggtc cgtcagtttc agccgcccct
     1621 ctgcgagccg ctggaaggtg atgtacagcg tcatcagctt ggtcagcgac gagggggtca
     1681 tctgctcgtc ggcgttcttt tccagcagcg tggtgccggt gttgaaatcg acaatgcagg
     1741 cccagcgcgc gtcggtggcg acctggccga tcggcgtctg agccggctcc ggcggcggct
     1801 gcgacggatc cggctgattg ccatgcgcgg cagcggccgg cccgcgcgtg ggatggcgcc
     1861 gttgtgccac agcgggtacc gcggcaagcg tcatggttga gccgagcaat atccggcgtg
     1921 tcagcatcgc gttgttctcc agcatcggtc tccccctact caacgacgat gcgcgcgtcg
     1981 gatacccccg cggcgattgc ccggtccagc gccgaatcgg ctgcggcgac atctgtgaag
     2041 ggacccagca tcacgcgaaa gctctgttcc cgattgtcga aaaccgattc gatgcgcggg
     2101 gccagtccga ccagcttggc acgctgcatc gctgcgtatt ggtaggactg aaaggtatcg
     2161 agtcgcaccc acaatcgacc gggccgcggc gattcctggg tcacggttcc gtccagccgc
     2221 acgctctcgg ctggcagcgc cgcggcgggc gcggccggca gcacggcctg gcgcccggct
     2281 tcctgacgca cgccggacgg cggcggcagc gcggcggcct ggacccggcc aaccggcgcg
     2341 gcggcgatcg acaggcgcgg tgcgccgcca accgcatcct gcacgctggc ggtctgcccc
     2401 ggcagcaggg tcagccgcac ttccgccacg ccatcgcgcg gcatgcccag cagggcggcg
     2461 acccgccggg tcacctgcac cagccggttc ggccggggcg tgccgcggtc gttgatccgc
     2521 accagcaggg agcgtccggt ctgcaggtcg gtcagccggg cgatggccgg cagcggcagc
     2581 gtgggatgcg ccacggccag cgcatcctgg ctgaacacct cgccatccgt ggtcagcgct
     2641 tgatgtccgc cggcatagat cacggcgagg ccggtcgcat ccagccttgg ctgttcgcgc
     2701 ggataccacc agacgccgcc ggactggtac ggctggccca ggacataatg cggatgcgcc
     2761 ggcggcgcct ggcggccgca ggaggcgagc agcaacgcca gggcgcaggc gcccagcgct
     2821 gccgcgaagc gggatgttct ggtgatcaca gtggggattg actgccggtt agcgtgccgg
     2881 ccgcgactcc gaccgtgacc aggcgtctat cacgcgccga actgctgccg ccagcgcaga
     2941 cccgcgacag tctccccggc gggacggtat tcgcaaccga cccagccctg gtagcccagc
     3001 tcatccatcc ggcggaacac gtagctccag ccgatctcgc cggtaccagg ctcattgcgt
     3061 gccggcacgt cggcgatctg catgtgggcg atcaccggca tgtactgctc catgcgcttg
     3121 gtgatgtcgc cctcggtcac ctgcacatgg tacacgtcga actgcagccc cacccggtcg
     3181 ggtcccaacg cttcgacgat cgccgcgccc tgcgcctggg tgttcaggtg atagccgggc
     3241 atgtcgcggt ggttgatcgg ctcgatgacg atcttcaccc cggccggcgc cgcctgctcc
     3301 gcggcccagg ccaggttggc ggcgtacacc gcggcggcgg tcaccggggt gacgccggcc
     3361 gccgggatgc ccgccatgca gtgcaacagc ttgcagtcca gcgcatgcgc atattccagc
     3421 gcgcgcttga cgctttcacg gaattccgtc tgccgcccgg gcaggctggc cagcccgcgg
     3481 tcgcccttcg cccagtcgcc gggcgccatg ttgaacagca cctgggtcag gccggcgccg
     3541 ctcaaccgcc ggcggatttc cgcggcggga aagtcgtagg ggaacaggta ttccacgccg
     3601 tcgaaatcgg ccttgcgggc ggcctcgaac cggtcgagga acggcacctc gttgaacatc
     3661 atcgacaggt tggcagcaaa gcgtggcaca gcgggtctcc cgagaagcgc gggcggaagc
     3721 tacaggcggg cgctgcgcag gccaagagtt gccggcagcc gaaccggccg ggttcagccg
     3781 acccactggc ggaacagcgc caagtcgata ttgccgccgc tgaccaccag accgacacgc
     3841 cgtccctgca tccgctcgcg ttcttgcaac aaggcggcga gtggcacggc accggcgccc
     3901 tccaccagat tatgcgtgtc ggtccagtac gcgcggatcg cgccgccgat ctcggcatcg
     3961 gtcaccgtga cgatgcgtgc ggcgccggcg cggatgatcg ccagcgcgcg cggatcgggt
     4021 tgccgcaccg ccaccccatc ggccagcgta tcggcgcggt tcagtgacac cacccggcct
     4081 tccgcgaagg atcgcgcata gcaatccgcg cccgccgcct gcacgccgat gatctccgtg
     4141 cccaggccca gcagatcgcg ggtgaggatg gcgccgcaga tgccggaacc cagtccgatc
     4201 ggcacataca gcgcatcgag cggcggcgtg ccgcggaaca gctccagaga ccaggtggcg
     4261 acgcctttca ccaggtcggg cgcgaaggac ggcacggctt ccaggccctc ggccgcggcg
     4321 aggcgcatgg cttcatcgcg cgccatctcg aaatcggtgc cgtgctcgat caaccgggcg
     4381 ccgaaggcac gcatcgcggc gttcttttcc acgctgttgc cctgcggcac ca
//
LOCUS       DASSZH010000125         2799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_276071, whole genome shotgun sequence.
ACCESSION   DASSZH010000125 DASSZH010000000
VERSION     DASSZH010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276071"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VFN42_02655"
     CDS             complement(<1..470)
                     /locus_tag="VFN42_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007006270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET8995545.1"
                     /translation="MPIQLTVNGVRHTVAAAETTPLLDVLRNHLDLKGARYGCGLEQC
                     GACMVLVDGEPVYACSREAGTVAGRSITTIEGLGSTDNPHRLQQAFLDEQAGQCGYCL
                     SGILMSAKALLDRNPSPTRAQIVEALDKHLCRCGAHPRILRAVEHAAAALRTGAA"
     gene            complement(498..1283)
                     /locus_tag="VFN42_02660"
     CDS             complement(498..1283)
                     /locus_tag="VFN42_02660"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HET8995546.1"
                     /translation="MAGTEVEWRRLRADQLREQAQRDAIVILPVASLEQHGPHLPVEV
                     DSLLGETVATRAARKLAARGQPALVLPVLWTGLSEHHMSFGGTVTLDFPAFSALVEGV
                     VRSVLRHGFRRIVLLNAHGGNENALRTITDDLTPKLRAPIVQFTYWYAAAVAIAKILE
                     TQGGLQHACEAETSMMMAVRPELVAAERIGMAKSNLTPDVGDVVGGGVYRWRSIASRS
                     GSGVIGNPEAASAEKGERLFEAIAEAVANKLGNAELWELPFLA"
     gene            complement(1311..1700)
                     /locus_tag="VFN42_02665"
     CDS             complement(1311..1700)
                     /locus_tag="VFN42_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HET8995547.1"
                     /translation="MNLLLDTHLLLWAAVEPNRLPRRARELLEDAQHRLMFSVASIWE
                     IAIKRGLERPDFLLQPGPFRRDLLENGYVEIPITGEHTVATMTLPPLHKDPFDRILVA
                     QAATEGYTLLTSDPKVAAYPGPIQYLR"
     gene            complement(1697..1945)
                     /locus_tag="VFN42_02670"
     CDS             complement(1697..1945)
                     /locus_tag="VFN42_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HET8995548.1"
                     /translation="MRTVNIHEAKTQLSRLVDQAAKGEPFVIAKAGKPLVKVVPLDAP
                     SRRKAQRLGFMQGAFTVPDDFDEMGSEQIERLFAGGSE"
     gene            complement(2017..>2799)
                     /gene="ribD"
                     /locus_tag="VFN42_02675"
     CDS             complement(2017..>2799)
                     /gene="ribD"
                     /locus_tag="VFN42_02675"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HET8995549.1"
                     /translation="PRVDGQGLARLREAGIVVEEGLLAAEAAEVNAGFFSRIRRGRPL
                     VTLKLASTLDGRIATRTGESRWITGEPARRAAHALRGRNDAVLVGVGTVMADDPDLTC
                     RLPGYRPIPLVRVVADSHLRTPLTMRLLATAKTTPTWMLIRAGTDPDRRHAVADLGVR
                     IMEVPGAPTGVDLAAGLRALGEAGITRLLVEGGAQIAAALLRAGLVDRIAWFHAPAVM
                     GGDGWPAVQAFGTQHLAEMPRFRRHRSMPLGEDMLSEFVRQD"
BASE COUNT          444 a          978 c          920 g          457 t
ORIGIN      
        1 gcggctcccg tgcgcagcgc ggcggcggcg tgttccaccg cacgcaggat gcgcggatgg
       61 gcaccgcagc ggcaaagatg cttgtccaac gcttcgacga tctgcgcccg cgtcggcgac
      121 ggattgcggt cgagcagcgc cttggcggac atcaggatgc cggacaggca gtagccgcac
      181 tggccggcct gctcatccag gaaggcctgt tgcagccggt gcggattgtc ggtgctgccg
      241 aggccttcga tcgtggtgat gctgcgtccg gccacggtac cggcctcacg cgagcacgcg
      301 taaaccggtt cgccgtccac cagcaccatg caggcgccgc attgttccag gccgcagccg
      361 tagcgcgcgc ctttcaggtc cagatggttg cgcagcacgt cgagcagcgg cgtggtttcc
      421 gccgcagcca ccgtgtgccg cacaccgttc actgtcagtt ggatgggcat cttggtcctg
      481 cttcctccgc cagactctca cgccagaaag ggcaattccc acaattccgc attgcccagc
      541 ttgttggcca ccgcttcggc gatcgcctcg aacagccgct cgcccttttc cgcgctggcc
      601 gcttcggggt tgccgatcac gccggatccc gaccggctgg cgatgctgcg ccagcgatac
      661 acgccaccgc cgaccacatc accgacatcc ggggtgaggt tggatttcgc catgccgatg
      721 cgctccgccg ccaccagctc cggccgcacc gccatcatca tggaggtctc cgcctcgcac
      781 gcatgctgca ggccgccttg cgtctcgagg attttcgcga tcgccaccgc ggcggcgtac
      841 cagtaggtga actgcacgat cggcgcgcgt agcttcggcg tgagatcgtc ggtgatggtg
      901 cgcagcgcgt tctcattgcc gccatgcgcg ttcagcagca cgatgcggcg gaagccgtgc
      961 cgcagcacgg agcggacgac gccttccacc agcgcgctga aggcgggaaa atccagggtg
     1021 acggtgccac cgaagctcat gtgatgctcg gacaggccgg tccacagcac cggcagcacc
     1081 agcgccggct ggccgcgcgc ggcgagcttg cgcgcggcgc gggtggcgac ggtttcgccg
     1141 agcagggagt cgacttcgac gggaagatgc gggccgtgct gttccagcga ggcgaccggc
     1201 aggatgacga tggcgtcgcg ctgcgcctgc tcgcgcaact ggtcggcccg caatcggcgc
     1261 cattccactt cagttcccgc catgacccgt ctccccgtcc tttgttgtgt tcacctcagg
     1321 tactggatcg gtcctggata ggcagcgacc tttggatcgg aggtgagcag ggtgtaccct
     1381 tccgtggctg cctgtgccac aagaatacgg tcgaagggat ccttgtgcag cggcgggagc
     1441 gtcatggtcg caaccgtgtg ctcgccagtg atcgggattt ccacgtaacc gttctccagc
     1501 aaatcacgac gaaacgggcc aggctgcagc aggaaatcgg gtcgttccag gccgcgcttg
     1561 atggcgatct cccaaatgct tgcgacgctg aacatcaagc ggtgctgtgc atcctccagc
     1621 agctcccgcg cgcgccgcgg caggcgattg ggctctaccg ccgcccatag gagcagatgg
     1681 gtgtccagca gcaggttcac tcagagccgc cggcgaacaa gcgctcaatc tgctcgctac
     1741 ccatctcatc gaaatcgtca ggcacggtga acgcgccctg catgaagccg agccgctgag
     1801 ccttccggcg acttggagca tccaaaggaa ccaccttgac cagcggcttc ccggccttgg
     1861 cgatcacgaa aggctctccc ttcgcggctt gatcgaccag gcgagacagt tgcgtcttcg
     1921 cctcatggat gttgacggtg cgcacggcca tcgtctcccg gcggactaag gctgatgaac
     1981 taagtctgat tgtctcgcca ggcaagccgg cgtcactcag tcctgccgga cgaactcgct
     2041 cagcatatcc tcgcccagcg gcatgctgcg atgccgccgg aagcgcggca tctcggccaa
     2101 atgctgcgtg ccgaaggcct gcacggccgg ccagccgtcg ccgcccatca ccgccggagc
     2161 atggaaccag gcgatgcgat ccaccagccc ggcgcgcagc aacgcggcgg cgatctgcgc
     2221 gccgccttcc accagcagcc gggtgatccc cgcctcaccc agcgcgcgca agccggcggc
     2281 gagatcgacg ccggtgggcg cacccggcac ctccatgatg cgcacgccca gatcggccac
     2341 cgcgtggcgg cggtccgggt cggtgccggc gcggatcagc atccaggtcg gcgtcgtctt
     2401 cgcggtcgcg agcagccgca tggtcagcgg cgtgcgcaga tggctgtcgg cgactacccg
     2461 gaccagcggg atggggcggt agccgggcag gcggcaggtc aggtcggggt catccgccat
     2521 caccgtgccg acgccgacca gcaccgcgtc gttcctgccg cgcagcgcgt gggcggcgcg
     2581 gcgggcgggc tcgccggtga tccagcggct ttcgcctgtg cgcgtggcga tgcggccgtc
     2641 cagcgtgctt gccagcttca gcgtcaccag cgggcggcca cggcggatcc ggctaaagaa
     2701 tccggcattg acctccgccg cctcggcggc aagcagccct tcctcgacca cgattcccgc
     2761 ttcgcgcaac cgcgccagcc cctggccatc gacgcgcgg
//
LOCUS       DASSZH010000126         2981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_514502, whole genome shotgun sequence.
ACCESSION   DASSZH010000126 DASSZH010000000
VERSION     DASSZH010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514502"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VFN42_02680"
     CDS             complement(<1..500)
                     /locus_tag="VFN42_02680"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HET8995550.1"
                     /translation="MLTLYFAPGASSMAPHIALHEIGVPFQAVPLSLRQQENRAAAYL
                     ALNPEGKVPTLLIDGRPLTEVAGILFYLARRFPDAGLLPADDLEAEAQVVSWMSFIAA
                     TIHPARRQGVQRWTTVFGFAEQRFGDGEWVLGRYSIADIHLFRLFWRFTRARVADAGA
                     FPRLEA"
     gene            complement(511..1278)
                     /locus_tag="VFN42_02685"
     CDS             complement(511..1278)
                     /locus_tag="VFN42_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995551.1"
                     /translation="MLRPGFWIVLLGVLAAGSALRAQPASAPAACPPFGDYAIPDEPL
                     SHVAAVLAAGGTLNVLAIGSATTVGVSASMVHGTAPGGSYPYRMADALQAARPHLQLG
                     LTVRGGRGMTAESMLPLLRQALKAQRYQLVLWQTSTVEAVHGDSPDAMQSALEDGAEQ
                     VLDSGADLVLVDSQFSRFLRANVDLDPYQSALQQVAAMPGVVVFRRFDLMRDWAHEGQ
                     LDLERAGRVDRPREIARLNTCLGQALARFVLNGAAQK"
     gene            complement(1284..2435)
                     /locus_tag="VFN42_02690"
     CDS             complement(1284..2435)
                     /locus_tag="VFN42_02690"
                     /inference="COORDINATES: protein motif:HMM:NF021633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HET8995552.1"
                     /translation="MTLIRLGTRRDLRVDFFRGLALWWIFVDHIPGNLMANYTLHDFA
                     VSDAAEVFVLLAGYGAGLAYGGTLNRQGYLFAAADTVKRAWTLYIAHIFLFVVFTAQV
                     AYSASLLDRAYYLDEARLDVLGTSPYRAMLEALLLRFQPSLLNILPLYVILLLFFALT
                     LWALRWPRLLLLGSFALYVLVRITGLTIPSWTGQDWFFDPFAWQFLFMIGAVLAYAPP
                     PMPRLRWPLDAVAVLVVLAGLLVIWIVKMHPPFVTAVPQGVIHFLIAQDKTGLHPGRL
                     LSVLSLTWLVVRLIPMDAAWLRSSAAGLLVLPGQNSLPVFCSGIFFGFIARLGLEYSD
                     DLWMQVVVNVFGAVAMLIVAAVTAWYRSKGRAPARTPAVLPLTVRTDTD"
     gene            complement(2506..>2981)
                     /locus_tag="VFN42_02695"
     CDS             complement(2506..>2981)
                     /locus_tag="VFN42_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cellulose synthase operon protein YhjQ/BcsQ"
                     /protein_id="HET8995553.1"
                     /translation="GSAALSGLDPAALEPTLVALRQAAVGYDATLLDLGAGVERGVRR
                     LAAFADRLLVVATEEPTSLTDAYAVLKLHAADRAAGDARILVNQAATPASGQRSYAAL
                     HRACTGFLKRDPPLAGIVRRDDHVREAIRRQALLLTRHPGCAAAQDIEVVAGGLM"
BASE COUNT          578 a         1033 c          980 g          390 t
ORIGIN      
        1 tgcgcttcca gcctcggaaa ggctcccgcg tccgcaaccc gcgccctggt gaagcgccag
       61 aacagccgga acagatggat gtcggcgatc gagtatcgcc ccaacaccca ttcgccatcg
      121 ccgaagcgct gctccgcgaa cccgaacacc gtggtccagc gctgcacgcc ctggcggcgc
      181 gccggatgga tcgtggcggc gatgaacgac atccaggaca ccacctgcgc ctcggcttcc
      241 aggtcgtccg ccggcaacag gccggcatcc ggaaagcgcc gcgccaggta gaacagaata
      301 ccggccactt cggtcagcgg acggccgtcg atcagcagcg tcggcacctt gccttccgga
      361 ttcagcgcca gataggccgc cgcgcggttc tcctgctgcc ggagcgacag cggcacggcc
      421 tggaacggca caccgatctc atgcaacgcg atgtgcggtg ccatcgaact cgcgccaggt
      481 gcaaaataca gcgtcagcat cgcacaaatc ctatttctgc gctgcgccgt tcagcacgaa
      541 gcgcgccagc gcctgtccca ggcaggtatt gagccgcgcg atctcccgcg gccggtcgac
      601 cctgccggca cgctccagat cgagctgccc ttcatgcgcc cagtcgcgca tcagatcgaa
      661 gcggcgaaac accacgacac ctggcatcgc ggccacctgc tgcagtgctg actggtaggg
      721 atcgagatcg acattggcgc gcaggaagcg gctgaactgg gaatccacca gcaccaggtc
      781 ggcgcccgaa tcgagcacct gttcggcacc gtcctccagt gcggactgca tggcatccgg
      841 cgaatcgccg tgcaccgcct ccaccgtgct ggtctgccag agcacgagtt gatagcgctg
      901 cgccttcagg gcctggcgca gcagcggcag catggattcc gcggtcatgc cgcggccacc
      961 gcgcacggtg agccccaatt gcagatgtgg ccgcgccgct tgcagcgcgt cggccatgcg
     1021 gtaggggtag gagccgcccg gcgccgtgcc atggaccatc gaggcagaca ctccgacagt
     1081 ggttgccgag ccgatcgcca ggacattcag cgtgccgccc gcggcgagca ccgcggcgac
     1141 atgcgacagg ggttcatcgg gaatggcgta gtcgccgaag ggggggcaag ccgccggggc
     1201 gctggccggc tgcgcgcgga gagcggagcc agccgcgagc acccctagca gcacgatcca
     1261 gaacccggga cgcagcatca attttaatcc gtatcagttc taaccgttaa cggcaatacg
     1321 gccggcgtac gggccggcgc tcggcccttg ctgcgatacc aggcggttac cgccgccacg
     1381 atcagcatcg cgacggcacc aaacacattc accacgacct gcatccacag atcgtcgctg
     1441 tattccagcc ccagccgggc aataaatcca aagaagatgc cggaacagaa caccggcagc
     1501 gagttctgtc cgggcagcac cagcagcccg gccgccgatg agcgcagcca cgccgcatcc
     1561 atcgggatca gccgcaccac cagccaggtc agactcagca ccgagagcag gcggcccgga
     1621 tgcagtccgg tcttgtcctg cgcgatcagg aagtggatca ccccctgcgg caccgcggtg
     1681 acgaacggcg ggtgcatctt gacgatccag atcaccagca gcccagccag cacgaccagc
     1741 accgccacgg catccagtgg ccagcgcagc cgcggcatgg gcggcggcgc ataggcgagc
     1801 accgcgccga tcatgaacag gaactgccag gcgaaaggat cgaagaacca gtcctgtccg
     1861 gtccagctcg gaatggtcag gccggtgatc cgcaccagca cgtacagcgc aaaggagccc
     1921 agcagcagca gcctcggcca gcgcagcgcc catagcgtca gggcgaagaa cagcagcagg
     1981 atgacgtaca gcggcaggat gttcagcagg ctgggctgga accgcagcag cagcgcctcc
     2041 agcatcgcgc ggtagggcga ggtgcccagc acgtccagcc gcgcctcgtc caggtagtag
     2101 gcgcggtcca gcaggctggc cgagtaggcg acctgagccg tgaagacaac gaacaggaag
     2161 atgtgagcga tatacagtgt ccaggcgcgt ttgacggtat ccgccgccgc gaagagataa
     2221 ccctgccggt tcagcgtgcc gccataggcc agaccggcgc catagccggc aagcagcacg
     2281 aaaacctccg ccgcgtcgct cacggcgaag tcgtgcaggg tatagttcgc cataagattg
     2341 cctgggatgt ggtcgacgaa aatccaccac agggccagcc cacggaagaa gtccacccgc
     2401 aggtcgcgcc tggtgccaag ccggatgaga gtcatgcccg ccccctatga ccggcaattt
     2461 gggcgttccc gcggcggagg ggaaccgggg gcgtttgaca aagcgttaca tcagcccacc
     2521 cgccacgacc tcgatgtcct gcgccgcggc gcagccgggg tgccgggtca gcagcaaggc
     2581 ctgccggcgg atcgcctccc gcacgtggtc gtcgcggcgg acgataccgg cgagcggcgg
     2641 gtcgcgcttc aggaaacccg tacaggcacg gtgaagcgcg gcgtagctgc gctggccgga
     2701 cgccggcgtg gccgcctggt tgaccaggat ccgtgcgtcc ccggcagccc ggtcggcagc
     2761 gtggagtttc agcacggcat aggcgtcggt caggctggtg ggctcctcgg tggcgaccac
     2821 cagcagccgg tcggcgaacg ccgccaggcg ccgcaccccg cgttccacgc cggcgccaag
     2881 gtcgagcagc gtggcgtcat accccactgc cgcctgacgc agcgcgacga gggtgggttc
     2941 cagcgcggcc gggtccagcc cggacagcgc agcgctgccg g
//
LOCUS       DASSZH010000127         2054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1059883, whole genome shotgun sequence.
ACCESSION   DASSZH010000127 DASSZH010000000
VERSION     DASSZH010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059883"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..696
                     /locus_tag="VFN42_02700"
     CDS             178..696
                     /locus_tag="VFN42_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET8995554.1"
                     /translation="MSFLVANFRHVAHGLQEGQFAVGEKRKAAGLDDLPPIYRQMLDE
                     PITMTLAITGPDGRPSLTPMWFDHADGEVLVNTASHRNKCQWIRDNPRLTILLVNPKN
                     PYHWMQIKCTVRRELREWDEGGDAVTRQTDKIWTKYTGQPGPYGLRDPKIDEKRVLFF
                     CGIDRIATFGEP"
     gene            complement(916..1848)
                     /locus_tag="VFN42_02705"
     CDS             complement(916..1848)
                     /locus_tag="VFN42_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HET8995555.1"
                     /translation="MNIVFHHTAGPDLAARLAAIPGLGISICPEDDDGLLMQRLPDCD
                     VLWHVLKRCTAAMIAAAPRLRLIQKIGVGVNTIDLDAARARGIPVCNLPGTNARAVAE
                     LTLGLMLAVLRRLVRFDTELRAGIWTDPMLQDGIGELGGRTVGLVGYGAIPRLLAPVL
                     AAMGCKVLHTSRRDVPDAIGRRVGLDTLLAQADIVSLHVPLTDDTAHMIDAAALARMK
                     PGAVLINTARGGLVDQPALVAALRSGQLGGAGLDVFEAEPAAPGDALFALPNVVVSPH
                     IGWLTTGTFDRSFALAAENCRRVAAGEPLLHRMV"
     gene            complement(1845..>2054)
                     /gene="eno"
                     /locus_tag="VFN42_02710"
     CDS             complement(1845..>2054)
                     /gene="eno"
                     /locus_tag="VFN42_02710"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012341.4"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET8995556.1"
                     /translation="AGIVSARSGETEDTTIVHLAVGWGTGQLKVGSFARGERMAKWNE
                     ALRIEEALGPRSVFAGGSLLGRSAA"
BASE COUNT          365 a          695 c          702 g          292 t
ORIGIN      
        1 tagatgacag gccgcgctgg catgacaagc tgttctcgcg cgccgggccg tcgcgcacgc
       61 cataccgatc catgacgccc atgcgcgaca ccgccaaacc gacatgatac gaatcccacc
      121 gggtgccaga cccggatgcc aaccgggcac gcgcatcaac cgcagggagg aacgaacatg
      181 tcatttttgg tggcgaattt ccgccatgtc gctcacggcc tccaggaagg ccagttcgcc
      241 gtcggcgaga agcgcaaggc cgccggcctg gacgatctgc cgccgatcta tcggcagatg
      301 ctggatgagc cgatcacaat gacgctggcc atcaccgggc cggacgggcg gccaagcctg
      361 acgccgatgt ggttcgacca tgcggatggg gaggtgctgg tcaacaccgc ctcccaccgc
      421 aacaagtgcc agtggatccg cgacaatccg cggctgacaa tcctgctggt caatccgaag
      481 aacccgtacc actggatgca gatcaagtgc accgtccggc gcgagttgcg cgagtgggat
      541 gaaggcggtg atgccgtgac gcggcaaacg gacaagatct ggacgaaata caccggccag
      601 ccggggccgt acgggctgcg cgatccgaag atcgacgaga agcgcgtcct gttcttctgc
      661 gggatcgacc ggatcgccac gttcggggag ccgtagcgga ccgtgcatga ccggccgagg
      721 cgcccgacca gctgagacac ctgccgggct gcggtgcctg accgccgagg cggtgcccag
      781 cggcatgacc gcaagcgcct tggccgccat cacacttggc ctccggcggg gattggacgc
      841 gccgtctggt gttcaagcct gattgcccgg ggctgaaagc cgcgaggcat taatcatgtg
      901 aggaagcgaa gacttctaga ccatccggtg cagcagcggt tcgccggccg cgacccggcg
      961 gcagttttcc gccgccaggg cgaagctgcg gtcgaaagtg ccggtggtca gccagccgat
     1021 atgcggtgag acgaccacgt tcggcagcgc gaacagagcg tcgccggggg ctgccggttc
     1081 ggcttcaaag acatccaggc cggcgccgcc aagctggccg gatcgcagcg cggccaccag
     1141 cgccggctga tccaccagcc cgccgcgcgc ggtattgatc agcaccgccc ccggcttcat
     1201 ccgggccagc gcggcggcat cgatcatgtg cgcggtgtcg tccgtcagcg gcacgtgcag
     1261 gctgacgata tcggcctgcg ccagcagggt atccagcccg acgcggcggc caatggcgtc
     1321 cggcacatca cgccgagagg tatggagcac tttgcagccc atggcggcga ggaccggggc
     1381 aagcaggcgc gggatcgcgc cgtagcccac cagcccgacc gtgcggccac ccagttcgcc
     1441 gatgccgtcc tgcagcatcg ggtcggtcca gatgccggcg cgcagttcgg tgtcgaagcg
     1501 gaccaggcgg cgcagcaccg ccagcatcag gcccagcgtg agttcagcga ccgcgcgtgc
     1561 gttggtgccg ggaaggttgc acaccgggat gccgcgcgcc cgcgccgcat ccaggtcgat
     1621 cgtgttcacg ccgacgccga tcttctggat caggcgcagg cggggtgcgg cggcgatcat
     1681 cgccgcggtg cagcgcttca gcacatgcca gagcacatcg cagtccggca gcctctgcat
     1741 cagcagcccg tcatcgtcct cggggcagat gctgatgccc aggccgggga tcgccgccag
     1801 gcgggcggcc aggtccggcc cggcggtgtg atggaagacg atgttcatgc ggccgagcgg
     1861 cccagcaacg atccgccggc aaatacgctg cggggaccca gggcttcctc gatgcgcaag
     1921 gcttcgttcc acttcgccat ccgctccccg cgggcgaaac tgccgacctt caactggccg
     1981 gtgccccaac cgacggcgag gtgaacgatg gtggtgtcct cggtctcccc ggaccgggcc
     2041 gagacgatgc cggc
//
LOCUS       DASSZH010000128         6542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1128236, whole genome shotgun sequence.
ACCESSION   DASSZH010000128 DASSZH010000000
VERSION     DASSZH010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6542
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128236"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VFN42_02715"
     CDS             <1..684
                     /locus_tag="VFN42_02715"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HET8995557.1"
                     /translation="DGRFVALAANWPGEPCPLHGNGLQARWQVTTHDARAAELTLDSD
                     GPGAFRYTATLRYWLQDGALGMRLAVRHRGAAPLPYGLGFHPWFPRTAATTLKARADA
                     VWLEDARHLPTRSVPIAGRPEWDFAGGRALPPGWINNGFVGWDGCATISWPDRGIALT
                     VNALAFEGSVPLSVYVLYSPRAEADAFCFEPVSHPVDAHNLPGLPGLLALAPGESVSV
                     QCRFVPQRL"
     gene            869..1707
                     /locus_tag="VFN42_02720"
     CDS             join(869..1259,1259..1707)
                     /locus_tag="VFN42_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612205.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HET8995558.1"
                     /translation="MWTNENRGRYDRSTLRYPSDLTDPEWALIGPLIPAAKRGGNKRT
                     VDVREIVNGVMYILSTGCQWAALPKDLPPRSTVNDYFRRWDYDGTLDRIHHALYVQCR
                     EHVGREASPTAAIIDSQSGKSAEKGGARFDPAGYDAGKKIKGKKRHILVDTQGLLMQA
                     IIHAADIQDRDGGMLLRGALFGLYPFLLKLYADSGYQGAKFQDGLKAACGRINLEIVK
                     RSDLHKFVVLPNRWIVERTIGWLNRCRRLAKDWECLNRNALAFLRWASVRLMLRKLCQ
                     TTL"
     gene            complement(1844..2701)
                     /locus_tag="VFN42_02725"
     CDS             complement(1844..2701)
                     /locus_tag="VFN42_02725"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET8995559.1"
                     /translation="MNDVTQVHADQLAYWNGAGGERWVVEQAHTDAMLAPVAEAAIAH
                     AAPRPGEHVLDIGCGCGMTTLALAEAVAPGGTVTGLDVSAPMLAVAQRRAEGRGQVAW
                     TLADAAAHDFADATFDLLFSRFGVMFFGEPTAAFANLRRAAQPGARLVFACWRALNDN
                     PWMLLPLQAAQTVLPPLPRPGPEDPGPFAFADTARVTRILTGAGWTAPTFTRLDVTLD
                     IAGGGGVEAATDHATRIGAAARLLADQPEAARQAAKDAIRTVLAPHATADGRVGLSGG
                     VWLVESRNG"
     gene            2823..4130
                     /gene="ahcY"
                     /locus_tag="VFN42_02730"
     CDS             2823..4130
                     /gene="ahcY"
                     /locus_tag="VFN42_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET8995560.1"
                     /translation="MPDTAVAQDYKVKDIALAEWGGKEIAMAEDEMPGLMAVRAEYGA
                     SKPLQGARIAGCLHMTIQTAVLIQTLEALGATVRWSSCNIFSTQDHAAAAIAAAGTPV
                     FAWKGMNEEEFWWCIEQTVRGPDGWTPNMILDDGGDLTLLMHDKYPAMLADVRGISEE
                     TTTGVHRLREMARAGTLRVPAINVNDSVTKSKFDNLYGCRESLVDAIRRGTDVMMAGK
                     VAVVNGFGDVGKGSAASLRNAGCRVMVTEVDPICALQAAMEGYEVTTMEDAAPAGDIF
                     VTATGNVDVITIDHMRRMKNRAIVCNIGHFDSEIQIEALRNHRWENVKPQVDEVVFPD
                     GKRLIVLSEGRLVNLGNATGHPSFVMSASFTNQVLAQIELWSAPEGKYQLDVYTLPKA
                     LDEKVASLHLAKVGAKLTQLSQKQADYIGVATSGPFKHDQYRY"
     gene            4386..4622
                     /locus_tag="VFN42_02735"
     CDS             4386..4622
                     /locus_tag="VFN42_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995561.1"
                     /translation="MSDSAVLPDFSRAQTDAVRRAMAASRDHALVADLLFLERWEMAP
                     IHGPAAALRMAQIRRANPTLAEQVRAEVARARRN"
     gene            5033..5740
                     /locus_tag="VFN42_02740"
     CDS             5033..5740
                     /locus_tag="VFN42_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HET8995562.1"
                     /translation="MAVEKVYPDAVSALAGLLRDGMTIMSGGFGLCGIPTTLIEAIRD
                     SGVRDLTVISNNAGIDDAGLGLLLQTRQIRKMISSYVGENQTFARQYLAGELEIEFNP
                     QGTLAERIRAGGAGIPAFFTRTGVGTLIAEGKEVREFDGRPYLMERSLFADLAIIHAW
                     KADTEGNLVFRKTARNFNPIMATAARVTVAEVEMLVEPGEIDPDHMHTPGVFVKRLTA
                     LVNVQKRIEQRTVRKRA"
     gene            5759..6409
                     /locus_tag="VFN42_02745"
     CDS             5759..6409
                     /locus_tag="VFN42_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HET8995563.1"
                     /translation="MAWTRDEMAARAARELQDGFYVNLGIGIPTLVANHIPPGISIQL
                     HSENGMLGMGPFPYEGEEDPDLINAGKQTVTELPTTSFFDSAASFAMVRGGHIDISIL
                     GALQVSEQGDLANWMIPGKMVKGMGGAMDLVAGVPRVVVLMEHTAAGKPKLLKHCTLP
                     LTGTKVVNLVVTDLGVFDVTPQGLVLVDMAPGVTVAELREKTEAGFAVHPDLKLAA"
BASE COUNT         1128 a         2210 c         2191 g         1013 t
ORIGIN      
        1 gatggccgct tcgtcgcgct ggccgccaac tggcccggcg agccgtgccc gctgcacggc
       61 aacgggttgc aggcgcgctg gcaggtcacg acccacgacg cgcgcgccgc cgaactcacg
      121 ctcgacagcg acggacccgg cgcgttccgc tacaccgcca cgctgcgcta ctggctgcag
      181 gacggcgcgc tggggatgcg gcttgccgtc cggcatcgcg gcgcggcgcc gctgccgtac
      241 ggcctcggct tccatccctg gttcccccgc accgccgcga cgacgctcaa ggcgagagcc
      301 gatgccgtgt ggctggagga tgcgcggcac ctgccgacgc ggagcgtgcc aatcgccggg
      361 cggcccgagt gggatttcgc cggcgggcgc gccttgccgc cgggctggat caacaacggc
      421 tttgtcggct gggacggctg cgccactata agctggcccg accgcggaat tgcgctcacc
      481 gtgaatgcgc tagcgtttga gggcagtgtt ccactctcag tctatgtcct ctattctccc
      541 cgcgcggagg cggatgcgtt ctgctttgaa ccggtgtcgc atccggtgga tgcccacaat
      601 ctccccggcc tcccaggcct attagccctc gcacccggcg aatctgtttc cgtgcagtgc
      661 cgcttcgtcc cacaaagatt atgataattc aactttaaca gggcgcagat attcgcggca
      721 cactgggcga gttctctgcg gctgcggatc aggaagtggc gatatctggc ccgcttcgga
      781 ctcgtctgag tgtccatccg ggaacacctt gagtcattgg aatcacttgt gattcgatag
      841 gcgcgatccg atttgccgga gccgcctgat gtggacgaac gagaaccgtg gtcggtatga
      901 ccgtagcacg ctgcgctacc cgagcgatct gaccgatccc gagtgggcgt tgatcgggcc
      961 attgatcccg gcggcaaagc ggggcggcaa caagcggacg gttgacgtgc gcgagatcgt
     1021 caacggggtc atgtacattc tgagcacggg ctgtcagtgg gccgcgctgc cgaaagacct
     1081 tccgccgcgc agcacggtga acgactactt tcgacgctgg gattacgacg gcaccctcga
     1141 tcgcattcac cacgcgctct atgtccagtg ccgcgaacat gtcgggcggg aagccagtcc
     1201 gacagcggcg atcatcgaca gccagagcgg caagagcgca gaaaaagggg gcgctcgatc
     1261 gacccggcgg ggtatgatgc aggcaagaag atcaaaggca agaagcggca catcctcgtc
     1321 gatacgcaag gtctgctgat gcaggcgatc atccatgccg ccgacatcca ggaccgcgac
     1381 ggcggcatgc tgctgagggg cgccctgttc gggctgtatc cattcctgct gaagctctac
     1441 gccgacagcg gctaccaggg ggcaaaattc caggacggat tgaaggcagc ctgcggccgg
     1501 atcaacctgg aaatcgtcaa acgctccgac ctccacaagt tcgtcgtgct gcccaaccgc
     1561 tggatcgtcg agagaaccat cggatggctc aaccgctgcc ggcgcctcgc caaagattgg
     1621 gagtgcctca accgcaatgc gctcgccttc ctccgatggg cctctgtccg cctgatgctc
     1681 agaaagctct gtcagaccac actatgatcc cggatggact ctaagaaccc tcaccccgac
     1741 cctctcccgc aagcgggaga gggagatgca ggcgaaatcc ccgaaatctt ccctctccca
     1801 cttgcggggt tgggctgcga agcgccgggt gagggatacg gcctcagccg ttccggctct
     1861 ccaccagcca cacgccgccg ctcagaccca cgcgcccgtc cgcggtcgca tggggtgcca
     1921 gcaccgtgcg gatggcgtcc ttcgcggcct gccgcgccgc ttccggctgg tcggccagca
     1981 gccgtgcggc ggcgccgatc cgggttgcgt gatccgtggc cgcctccacg ccgcccccgc
     2041 cggcgatgtc caatgtcacg tccagcctgg tgaacgtcgg cgcagtccat ccggcgccgg
     2101 tcaggatgcg cgtgacgcgc gccgtgtcgg cgaaggcgaa cgggccggga tcttccggtc
     2161 cgggccgcgg cagcggcggc agcacggtct gtgccgcctg cagcggcagc agcatccacg
     2221 ggttgtcgtt cagcgcccgc cagcaggcga acaccagccg cgcgcccggc tgcgccgccc
     2281 gccgcagatt ggcgaaggcg gcggtcggct cgccgaagaa catcaccccg aagcgcgaga
     2341 acaacaggtc gaaggtggcg tcagcaaagt cgtgcgccgc cgcgtcggcc agcgtccagg
     2401 cgacctggcc cctcccttcc gcccgtcgtt gcgccaccgc cagcatcggc gccgacacgt
     2461 ccaatcccgt caccgtcccg cccggtgcca ccgcctcggc cagcgccagc gtggtcatgc
     2521 cgcagccgca gccgatgtcg agcacatgct cacccggacg cggcgccgcg tgggcgatcg
     2581 ccgcctcggc gaccggggcc agcatggcgt cggtatgcgc ctgctccacc acccagcgct
     2641 cgccgcccgc gccgttccag taggccaact ggtcggcatg cacctgcgtc acgtcgttca
     2701 ttcgactctc ccgcaccgtc gccgcacacc tatgcccggc cggggaagcc ggcaagcgcg
     2761 gggaaagatt gcggacataa ggataccctt atatatcggc cgggatttgc caggagccag
     2821 ccatgccgga taccgccgtc gcccaggact acaaggtcaa ggacatcgcg ctcgccgaat
     2881 ggggcggcaa ggaaatcgcg atggccgagg acgagatgcc cggcctgatg gcggtgcgcg
     2941 cggaatacgg cgcgtccaag ccgcttcagg gcgcgcggat cgccggttgc ctgcacatga
     3001 ccatccagac cgcggtgctg atccagacgc tggaggcgct gggcgccacc gtgcgctggt
     3061 cgtcctgcaa catcttctcc acccaggacc acgccgccgc cgcgatcgcc gccgccggca
     3121 cgcccgtttt tgcctggaag ggcatgaacg aggaggagtt ctggtggtgc atcgagcaga
     3181 ccgtccgcgg gccggatggc tggacgccga acatgatcct cgatgacggc ggcgacctga
     3241 cgctgctgat gcacgacaag tatcccgcga tgctcgccga cgtccgcggc atttccgagg
     3301 agaccacgac cggcgtgcac cgcctgcgcg agatggcgcg cgccggcacg ctgcgcgtgc
     3361 ccgcgatcaa cgtcaacgac agcgtcacca agagcaagtt cgacaacctg tacggctgcc
     3421 gcgagagcct ggtcgatgcc atccgccgcg gcaccgacgt gatgatggcc ggcaaggtcg
     3481 ccgtggtgaa cggcttcggc gacgtgggca agggttcggc cgccagcctg cgcaacgccg
     3541 gctgccgcgt gatggtcacc gaggtcgatc cgatctgcgc gctgcaggcg gcgatggagg
     3601 gctacgaggt gacgacgatg gaggatgccg cgccggcggg tgacatcttc gtcaccgcca
     3661 cgggcaatgt cgacgtcatc acgatcgacc acatgcggcg catgaagaac cgcgccatcg
     3721 tctgcaacat cggccatttc gactccgaga tccagatcga ggccctgcgc aaccaccgct
     3781 gggagaacgt caagccgcag gtggacgagg tggtgttccc cgacggcaag cggctgatcg
     3841 tgctgtccga gggccgtctg gtgaatctcg gcaacgccac cggccatccg agcttcgtga
     3901 tgagcgccag cttcaccaac caggtactgg cccagatcga actgtggagc gcgccggagg
     3961 gcaagtacca gcttgatgtc tacacgctgc cgaaggcgct ggatgagaag gtggcgtccc
     4021 tgcatctcgc caaggtcggc gccaagctga cgcagctgtc acagaagcag gccgactata
     4081 tcggcgttgc gacgagcggc ccgttcaagc acgatcagta tcgttactga ttggaatatg
     4141 ccgggtggcg tgaaacggtt tagccacccg gcgatttcgt tctgacattt tcctgcgacg
     4201 acattcagag ttcgttaacg agcgcgtgac gtcatgccga agtcacgaag ctgtcgctcg
     4261 cccgtcgcgt ggcgcgggct aaaacaaggg tgccggctga accgccgagc ctgacggacg
     4321 ctgttgcagg cgtccggccg ggcccgggtc tgtccggcgc agtgtttccc ttgaggtagt
     4381 ccagtatgtc cgactcggcc gtcctgcccg atttctcccg ggcgcagacc gatgcggtcc
     4441 gtcgtgccat ggccgcatca cgcgatcacg cgctcgtcgc agacctgctg ttcctggagc
     4501 gctgggaaat ggctcccatc cacggtcccg ccgcggcgct gcgcatggcc cagatccgcc
     4561 gcgccaaccc aaccctcgcc gaacaggtgc gcgccgaggt cgcgcgcgcg cgccggaact
     4621 gagccgcccg gccgcctttt acccggcgcc tgaggccgcc cgggacatag caatctgtcc
     4681 cgggcggccc agtttgcgcc ctgttccgcc aagtacagcc gaacaaagca gaagacagcg
     4741 cccggtccgg atgccagcaa gtacgcgggc atttccggga ccggcgaatg gaccgagtac
     4801 accgccgccc ccatgaaggc ggatggcaag ttctaccggg gaatcgtctt caataacgcc
     4861 cactgggtcc tgaaggagcg gcgcccggca gcggccggcg atcgacgggc ggcagggccg
     4921 gcgcgcccgg tcgtgtcgcc cgctccgtcg gggctgtcgt accgacacgt tgcatccttc
     4981 ccgccagggc ggcattttgc ccccgcggac gagcggcagg aaaggcgaag cggtggcggt
     5041 cgagaaagtc tatcccgatg cggtttcggc gctggcgggg ctgctgcgcg acggcatgac
     5101 gatcatgtcc ggcgggttcg gcctgtgcgg catcccgacc acgctgatcg aggcgatccg
     5161 cgattccggg gtgcgcgacc tgacggtgat ctccaacaac gccggcatcg atgacgcggg
     5221 cctcggcctg ctgctgcaga cgcggcaaat ccggaaaatg atcagctcct acgtgggcga
     5281 gaaccagacc ttcgcccggc agtacctcgc gggcgagctg gagatcgagt tcaacccgca
     5341 gggcacgctc gccgagcgca tccgcgccgg cggcgccggc atcccggcct tcttcacccg
     5401 caccggcgtc ggcacgctga tcgccgaggg caaggaggtg cgggagttcg acggcaggcc
     5461 ctatctgatg gagcgcagcc tgttcgccga tctcgccatc atccacgcct ggaaggcgga
     5521 taccgagggc aacctcgtgt ttcggaagac ggcgcggaac ttcaacccga tcatggcgac
     5581 cgcggccagg gtcacggtgg ccgaggtgga gatgctggtc gagccgggcg agatcgaccc
     5641 cgaccacatg cacacgcccg gcgtgttcgt caaacggctg accgcgctgg tcaacgtgca
     5701 gaagcgcatc gagcagcgca ccgtgcgcaa gcgcgcctga ggcaagggag gaccggacat
     5761 ggcctggacc agggacgaga tggcggcgcg ggccgcgcgc gagttgcagg acgggttcta
     5821 cgtcaatctc ggcatcggca tccccacgct ggtcgccaac cacatcccgc ccggcatctc
     5881 catccagctg cacagcgaga acggcatgct gggcatgggg ccgttcccgt acgagggcga
     5941 ggaggacccc gacctcatca acgccggcaa gcagacggtg acggagctgc cgaccaccag
     6001 cttcttcgac agcgccgcca gcttcgccat ggtgcgcggc gggcatatcg acatctcgat
     6061 cctcggcgcg ctgcaggtct ccgagcaggg cgacctcgcc aactggatga tccccggcaa
     6121 gatggtgaag gggatgggcg gcgcgatgga cctggtggcc ggggtgccgc gcgtggtggt
     6181 gctgatggag cacaccgccg ccggcaagcc gaagctgctg aaacactgca cgctgccgct
     6241 gaccgggacg aaggtggtca acctggtcgt caccgatctg ggcgtgttcg acgtgacgcc
     6301 gcaggggctg gtgctggtgg acatggcgcc gggggtcacg gtcgcggaac tgcgcgagaa
     6361 gaccgaggcg gggttcgcgg tccaccccga tctgaaactg gcggcctgag tcgcgcgaca
     6421 gcctcttgat cgaggggcgg ttgccggcct atatgcccgg ctcccgcacg ggagccgcca
     6481 gccgcggtcc ggggccatag ctcagttggg agagcgcctg aatggcattc aggaggtcag
     6541 gg
//
LOCUS       DASSZH010000129         5231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_105381, whole genome shotgun sequence.
ACCESSION   DASSZH010000129 DASSZH010000000
VERSION     DASSZH010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105381"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2261)
                     /locus_tag="VFN42_02750"
     CDS             complement(<1..2261)
                     /locus_tag="VFN42_02750"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET8995564.1"
                     /translation="MLDRKLLRDVLRLRGQVITIAAVIASGIAIFVSSLATYSSLRQN
                     QAAYYQAAHFAQVFVSLKRAPDALLPRLRALPGIAQIEPRIVHDITIDLPDQALPISG
                     RLVSLPVDASAPRLDRLYLRRGRLPTPRAANEVLVNEAFAAANNLRPGNSLAAILNGK
                     LQQLRIVGVALSPEYVFATRAGDAIPDDKRFGVLWMDHDAAAAAFDMTGAFNDVVVSL
                     APGTRAAAVLRGLDRLLMPYGGLVAYERRDQSSNRFLSDEISQQGTMATTIPVVFLLV
                     SAFLLNVVLSRLVTSQREQIASLKAIGYATGPIAFHYLKLAGVVILGGALPGVGLGIV
                     LGWLLTRNYTNFFRFPLLAFHLPLWAPLLAVAVALLAGSSGVLSAVRAVVRLAPAEAM
                     RPPTPLQYRAGWLDRVLGLRFLSARHRITLRGITGRPMRAALTVLGVSLAVPVILVSR
                     FWNDALDYMIDVQFAAVDRGDVTVTFTGPVSATARWDIARMPGVLLTEGFRSVPVRLV
                     AGHRTYRTAILGLPQDGALRRPLDSHLRPVPLPPDGVLLSQRLAERLHVRPGEHIRLE
                     VLEGERPERDVPVAGLADDMIGLNAYMRLDALNRLMREGDTINAVSLRTDHRPADALY
                     RALKAAPKVETVTVSALALQLFRATTVFFVLVMAGIFTLFSTVIAIGVVYNNARIALQ
                     ERGWELATLRVLGFTRAEVAALLLAEIGLEVIAGIPLGLWFGVWLVRGMIVLNETEMF
                     RIPAIIETRSYLLA"
     gene            complement(2264..3025)
                     /locus_tag="VFN42_02755"
     CDS             complement(2264..3025)
                     /locus_tag="VFN42_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8995565.1"
                     /translation="MFNRQPRQEIGPEPPGPVRGGPIFIARKLSKTYRAGEVLIPALR
                     GIDLEIAAGEFIVILGPSGSGKSTLLNILGGLDTPSGGEAMFREHRLTNATEAELTRY
                     RREHVGFVFQAYNLIPSLTARENVALVTEIAANPMPPEEALRRVGLQQRLDHFPAQLS
                     GGEQQRVAIALAIAKRPDVLLCDEPTGALDCQTGITVLRAILDSNRQLGTTAVVITHN
                     AAIAGMADRVLYLADGTIARSETNASRREPEEIAW"
     gene            3137..4261
                     /locus_tag="VFN42_02760"
     CDS             3137..4261
                     /locus_tag="VFN42_02760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET8995566.1"
                     /translation="MDTHDTDVVVIGAGIAGASAAASLTADHRVALIEAEDVAGYHST
                     GRSAAVWIQNYGPPDVRMLTGLSRTFFEQPPAGFAEVPLMARRPVVFLAAEDQCATLA
                     AMLAEGRGLRELGIAELREMVPALRPGYAVAAAIEDDAFDMDVAALHQGFLRQLRARG
                     GMLALRRRAGRITRENGRWEVEASGGARFRAKALVNAAGAWGDQVAGLAGVAPLGLVP
                     CRRTGVIIDPAPWQVADWPLLDGLGHGWYTRPEARTRLMVSPADETPLPPQDVQPDEL
                     DIAIAIDRMQQALDIPVHRVERSWAGLRTFTPDHSLAFGWDAQAEGFFWCVGQGGYGI
                     QTSPAAGKLVADLIAGRDVGDAATILPAIDPRRFVQSPAA"
     gene            4364..>5231
                     /locus_tag="VFN42_02765"
     CDS             4364..>5231
                     /locus_tag="VFN42_02765"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET8995567.1"
                     /translation="MVTSTESDELVRVGPGTPMGDLMRQYWLPALASSEVKAGGEPVR
                     LMLLGEKLIAFRDSSGRVGVFDHRCPHRCASLFYGRNEEGGIRCIYHGWKFDTDGNCL
                     DMPNVVPRQRSEEKVHAKAYKVTERAGLIWVYMGTRAEAPPMPAIEATLLDDAEIDLM
                     FVQRECNWLQALEGDIDTSHFTFLHVGSLQPEQIDDGNMMKYQVADTDWGTMYTAYRP
                     AEEDRTYWRFAHFGFPFWTWVPQGDFLDRVSARAWVPMDDEHTMFLSISWKKMSRMPP
                     LKDGKPIPGSRAK"
BASE COUNT          873 a         1782 c         1807 g          769 t
ORIGIN      
        1 gcgagcaggt aggagcgcgt ctcgatgatc gccggaatgc ggaacatctc cgtctcgttc
       61 agcacgatca tgccgcgcac cagccacacg ccgaaccaca ggcccagcgg aatgccggcg
      121 atgacttcca ggccgatctc cgccagcagc agcgctgcca cttccgcccg ggtgaagccg
      181 agcacgcgca gtgtggcgag ttcccagccg cgctcctgca gggcgatgcg ggcgttgttg
      241 tacaccacgc cgatggcgat cacggtggag aacagggtga agatccccgc catcaccagc
      301 acgaagaaca ccgtggtggc gcgaaatagc tgcaaggcca gggcactgac cgtgacggtt
      361 tccaccttgg gtgcggcttt cagcgcgcgg tacagcgcgt ctgccggacg gtggtcggtg
      421 cgaaggctga cggcgttgat cgtatcgccc tcgcgcatca gccggttcaa cgcgtccagc
      481 cgcatatagg cgttcaggcc gatcatatcg tccgccagcc cggctaccgg cacgtcgcgt
      541 tccggccgct cgccttccag tacctccagc cggatgtgct cgcccggacg cacatgcagc
      601 cgctcggcca gacgctgcga cagcaggacg ccatcgggcg gcaaaggaac cggacggaga
      661 tggctgtcca gcggccggcg cagtgcgccg tcctgcggca ggccgaggat cgccgtgcgg
      721 taggttcgat gccctgcgac cagtcgcacc ggcacggacc ggaaaccttc ggtcagcagc
      781 acgccgggca tccgggcaat gtcccagcgc gccgtggcgg agactggacc ggtgaaggtc
      841 accgtgacat cgccgcgatc caccgccgcg aactgcacgt cgatcatgta gtccagagcg
      901 tcgttccaga accggctgac caggatcacc ggcacggcca gcgagacccc gagcacggtg
      961 agcgcggcgc gcatcggccg gccggtgatg ccgcgcaacg tgatccggtg gcgcgccgag
     1021 aggaaccgca ggcccagcac gcgatccagc cagccggcgc ggtattgcag cggggtcggc
     1081 gggcgcatcg cctcggccgg tgccaggcgc accaccgcgc gcaccgccga caagacgccg
     1141 ctgctgccgg ccaacagcgc cacagcgacc gccagcagcg gcgcccaaag cggcaggtgg
     1201 aacgccagca gcgggaagcg gaagaaattg gtgtagttac gggtcagcag ccagcccagg
     1261 acaatgccca gcccaacccc gggcagcgcg ccgcccagga tcacgacccc ggccagcttc
     1321 aggtagtgaa aagcaatggg acccgtggca tagccgatcg ccttcaggct ggcgatctgc
     1381 tcgcgctggc tggtcacgag ccggctcagc acgacattca acaggaaggc tgagaccagc
     1441 aggaacacca ccgggatggt ggtggccatg gtgccctgct ggctgatctc gtcggacagg
     1501 aagcgattgg acgactggtc gcggcgctca taggcaacca gcccgccata gggcatcagc
     1561 agccggtcca gcccgcgcag cactgcggcg gcacgcgtgc cgggcgccag cgacaccacc
     1621 acatcattga atgcgccggt catgtcgaac gccgcggcgg ccgcgtcgtg atccatccac
     1681 agcacgccga agcgcttgtc gtccgggatc gcgtcgccgg cccgggtggc gaagacgtat
     1741 tccggcgaca gcgccacgcc gacgatgcgc aactgttgca acttgccgtt cagaatcgcc
     1801 gccagactgt tgccgggacg cagattgttg gcggcggcga aggcctcgtt caccagcacc
     1861 tcgttggcgg cacgcggcgt cggcaggcgg ccgcggcgca gatacagccg gtccagccgc
     1921 ggcgcagagg catccaccgg cagcgacacc agccggccgg agatcggcag cgcctggtcc
     1981 ggcaggtcga tggtgatgtc gtgcacgatg cgcggctcga tctgggcgat gccgggcagc
     2041 gcccgcagcc gcggcagcag cgcgtccggc gcgcgcttca gggagacgaa cacctgggcg
     2101 aaatgcgccg cctggtaata cgcggcctgg ttctgccgca gcgaggagta ggtggcgagc
     2161 gacgacacga agatcgcaat gccgctggcg atcaccgcgg cgatggtgat cacttggccg
     2221 cgcaggcgca gcacgtcgcg cagcagcttg cggtccagca tgctcaccag gcgatctcct
     2281 ccggttcgcg gcgggacgcg ttggtttcgc tgcgggcgat ggtgccgtcc gccaggtaca
     2341 gcacgcgatc cgccatgccg gcgatcgccg cgttgtgggt gatcaccacg gcggtggtgc
     2401 caagctggcg gttgctgtca agaatggcgc gcaacacggt gatccccgtc tggcagtcca
     2461 gcgcgccagt cggctcgtca cacagcagca cgtcgggccg cttggcgatc gcgagagcga
     2521 tggcgacgcg ctgctgctcg ccgccagaca gttgtgccgg gaagtggtcc agccgctgtt
     2581 gcagcccgac gcggcgcagc gcctcctccg gcggcatcgg gttggcggcg atctcggtga
     2641 ccagcgcgac attctcccgc gcggtcaggc tggggatcag gttgtacgcc tggaacacga
     2701 agccgacatg ctcccggcgg tagcgggtga gttcggcctc cgtcgcgttg gtcagccggt
     2761 gttcgcggaa catcgcctcc ccgccgctgg gcgtatccag gccaccgagg atgttcagca
     2821 gcgtggactt gccgctgcct gacgggccga ggatgacgat gaactcgccg gcggcgatct
     2881 ccagatcgat gccgcgcagc gccgggataa gcacctcgcc ggcgcggtag gtcttgctca
     2941 gcttgcgcgc aatgaagatc gggccgccgc gtactgggcc gggcggctcc ggtccgatct
     3001 cctggcgggg ctggcggttg aacatcgctg ttctccggcg ccgtcatgcg cgcccttggc
     3061 cgccggggcg atgacctgga tcaaccggga accggcgaag gttgcacccc gtgccggcac
     3121 gccggatggt gcaccgatgg acacgcacga taccgacgtg gtggtgatcg gcgccgggat
     3181 tgccggtgcc agcgccgcag cctctcttac cgccgatcac cgtgtggcgc tgatcgaagc
     3241 ggaggacgtg gccggctacc acagcaccgg ccgctctgcc gccgtctgga tccagaatta
     3301 cggcccgccc gacgtacgga tgctgaccgg gctttcccgc acattcttcg agcagccgcc
     3361 ggccggcttt gccgaggtgc cgctgatggc gcgccgcccg gtggtgtttc tcgccgcgga
     3421 ggaccagtgc gccacgctgg cggccatgct ggcggaaggc cgcggcctgc gcgaactcgg
     3481 cattgccgaa ctgcgggaaa tggtccctgc cctgcggccc ggttacgccg ttgcggcggc
     3541 gatcgaggac gatgccttcg atatggacgt cgcggcgctg caccagggct tcctccggca
     3601 gcttcgcgcc cgcggcggca tgctggcctt gcgccgccgc gccggacgca tcacccgcga
     3661 aaacggacgc tgggaggtgg aagcctccgg cggcgcgcgg ttccgcgcca aggcgctggt
     3721 gaacgccgcc ggagcctggg gcgaccaggt ggccggtctt gccggcgtcg cgccgctcgg
     3781 cctggtaccg tgccgccgca ccggggtgat catcgacccc gcgccctggc aggtggccga
     3841 ttggccgctg ctcgatggtc tcggccatgg ctggtacacc cggcccgaag cacgcacgcg
     3901 gctgatggtc tcgccggcgg acgagacccc cctgcccccg caggacgtcc aaccggacga
     3961 gctggacatc gccatcgcca tcgaccgcat gcagcaggcg ctggatatcc ccgtccaccg
     4021 ggtggagcgc agctgggcgg ggctgcgcac cttcacgccc gaccacagcc tggcgttcgg
     4081 ctgggacgcg caggcagaag gcttcttctg gtgcgtcggc cagggcggct acggcatcca
     4141 gacctctccc gccgccggca agctggtcgc ggacctgatc gccggtcgcg atgtgggaga
     4201 cgcggcaacg atcctgcccg ccatcgatcc gcgacgcttc gtccagtcac cggcggcata
     4261 gcaagggaag gacccatacc gccgcttgac cggccctcgc ccgagcctaa gctttcggca
     4321 acggacgcgc cggcatcgac cgcgcccgca agggaggaaa accgtggtca catccaccga
     4381 aagcgacgaa ctggttcgcg tcggcccagg cacccccatg ggcgacctga tgcgccaata
     4441 ctggcttccg gcgctcgcct catccgaggt gaaagcaggc ggcgagccgg tccgcctgat
     4501 gctgctgggt gagaagctga tcgccttccg cgacagcagc ggccgtgtcg gtgtgttcga
     4561 ccaccgctgc ccgcatcgct gcgcgtcgct gttctatggc cgcaatgagg aaggcggcat
     4621 ccggtgcatt tatcacggct ggaagttcga cacggacggc aactgcctgg acatgccgaa
     4681 tgtggttccg cgccagcgtt ccgaggagaa ggtgcatgcc aaggcctaca aggtcacgga
     4741 acgtgccggg ctgatctggg tgtacatggg cacccgcgcc gaagcgccgc cgatgccggc
     4801 gatcgaggcc acgctgctgg acgatgccga aatcgacctg atgttcgtgc aacgggaatg
     4861 caactggctg caggcgctgg aaggcgacat cgacacgtcg catttcacct tcctgcatgt
     4921 cggcagtctg cagccggagc agatcgacga cggcaacatg atgaaatacc aggtggcgga
     4981 caccgattgg ggcacgatgt acaccgccta ccgcccggcg gaggaagatc gcacctattg
     5041 gcgctttgcg catttcggct ttccgttctg gacctgggtg ccgcagggcg acttcctgga
     5101 tcgcgtgtcg gcacgcgcct gggtgccgat ggacgacgag cacaccatgt tcctgtcgat
     5161 ttcctggaag aagatgtcgc gcatgccgcc gctgaaggac ggcaagccga tcccgggctc
     5221 acgcgcaaaa c
//
LOCUS       DASSZH010000130         4385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_105392, whole genome shotgun sequence.
ACCESSION   DASSZH010000130 DASSZH010000000
VERSION     DASSZH010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105392"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VFN42_02770"
     CDS             complement(<1..182)
                     /locus_tag="VFN42_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995568.1"
                     /translation="MTDSALRRALRRARDGVTLDATEAEILLAARDAALDDLCASAAR
                     VRDSGLAAAGRPGVVT"
     gene            complement(188..1186)
                     /gene="fgd"
                     /locus_tag="VFN42_02775"
     CDS             complement(188..1186)
                     /gene="fgd"
                     /locus_tag="VFN42_02775"
                     /EC_number="1.1.98.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003619205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase
                     (coenzyme-F420)"
                     /protein_id="HET8995569.1"
                     /translation="MLKLGYKASAEQFDPRRLLAFSCAAEQAGFDSVFVSDHFQPWKH
                     TDGHAPSALAWLGALGASTSRIVMGTSVLTPTFRYHPSVVAQVFGTLGEMFPGRVVLG
                     VGTGESLNEVPAIGIKWPEMKERFAHMREAIGLIRKLWAEDRVSFEGQYYRTENATVY
                     DKPGTPVPIYVAGAGPMVAKYAGRSADGFICTSGKKWDLYTETLLPNVEEGIKAAAQP
                     KPEYDRLIEMKVSFDTDRDRALQDTRHWAALALTPEEKMSVEDPAEMEKLADALPVER
                     AASRWIVSSDPEEHVEKIRPYVELGFNHLVFHAPGPDQEKFLRLYGTEILPRLRSL"
     gene            1369..3648
                     /locus_tag="VFN42_02780"
     CDS             1369..3648
                     /locus_tag="VFN42_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HET8995570.1"
                     /translation="MAEPEHKGVQVQVANARPEDVGKGVARLSRGVMETIGVREGDAI
                     EIAGHRHTAALVLSPAPEDEGIEIIRLDGLERANAGVSAGERVEVRRAPVRPAGRVTI
                     APAQKNLRLSGSGDALRRTLIGRPLMAGDVISTSVYQRPVAAGDGVPEELMRHFLQQQ
                     SYALQEIRLTVVSTTPRGIVRIEPDTEIELLPEYAEPKETRRADVTYDDIGGLGNAVE
                     QVREMVELPLRHPELFQRLGIDPPKGVILHGPPGTGKTLLAKAVANESEAAFFSIAGP
                     EIMGSHYGESEQRLRDIFQEAGRRSPAIIFIDELDSIAPKRQEARGEVERRIVAQLLT
                     LMDGLEARQNVVVIAATNRVDALDEALRRPGRFDREIVIGVPDQDGRRQVLAIHTRGM
                     PLHEDVDLDGLARSTYGFVGADVAALAREAAMDAVRRVLPQINLRDGIPAELLAGMRV
                     CREDFDNALKRVQPSALREIMIQVPNVTWEDVGGLQETREQLRDGIELPLKNPEAFRR
                     LGIRPAKGFLLFGPPGTGKTLMAKAVAREANANFVATKSSDLLSKWYGESEQQISRLF
                     ARARQVAPTVIFIDEIDSLVPARGGGLGEPAVTERVVNTILAEMDGLEDLQGVVVIGA
                     TNRPNLLDPALLRPGRFDELVYVPVPDEAGRLHILQIQTSGMPLAEDVDLKRIAERSK
                     GYTGADLGDVVRRAGLLALRENLQSDMVHAAQFEQAMRATRASVTPEMEREYEDLRGH
                     LKEQGPTSRQPIGFAMGTG"
     gene            3750..3968
                     /gene="infA"
                     /locus_tag="VFN42_02785"
     CDS             3750..3968
                     /gene="infA"
                     /locus_tag="VFN42_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET8995571.1"
                     /translation="MSKEDMIEFSGTVMELLPNAMFRVKLDNEHTILAHTSGKMRKNR
                     IRVLAGDRVNVEMTPYDLSKGRITFRFK"
     gene            3989..>4385
                     /locus_tag="VFN42_02790"
     CDS             3989..>4385
                     /locus_tag="VFN42_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HET8995572.1"
                     /translation="MPPLVLASASPRRLALLAQIGVTPARVLPPDIDETPQRDELPRL
                     YAQRLARAKAAAVAAPGCAVLAADTVVAAGRRILPKADTAAQARRCLALLSGRRHQVM
                     TAVVLGMPDGRSSERLATSIVSFARLTEPQ"
BASE COUNT          728 a         1494 c         1521 g          642 t
ORIGIN      
        1 taggtgacga caccggggcg gccggcagca gcaagtcccg agtcgcggac gcgggccgct
       61 gacgcgcaca gatcgtccag cgccgcgtca cgagcggcca gcaggatctc ggcctcggtc
      121 gcgtccagcg tcacgccgtc gcgcgcgcgc cgcagcgccc tgcgcagagc agagtccgtc
      181 acgttcgtca caagctgcgc aacctcggaa gtatttccgt gccgtacagg cgcaggaatt
      241 tctcctggtc cggccccggc gcatggaaca ccaggtggtt gaagcccagc tcgacatagg
      301 gacggatctt ttccacgtgc tcctccgggt cgctggagac gatccagcgg ctggcggcac
      361 gctcgaccgg cagcgcatcg gccagcttct ccatctcggc ggggtcttcc accgacatct
      421 tctcctccgg agtcagcgcc agcgccgccc aatggcgcgt gtcctgcaac gcgcggtcgc
      481 ggtcggtatc gaacgaaacc ttcatctcga tcagccggtc gtattccggc ttcggctgtg
      541 ccgccgcctt gatgccttct tcgacattcg gcagcagcgt ctcggtgtag agatcccact
      601 tcttgccgct ggtgcagatg aacccgtccg cgctgcgccc ggcatatttc gccaccatcg
      661 ggccggcccc ggcgacatag atcggcaccg gcgtgcccgg cttgtcgtac accgtggcgt
      721 tttccgtgcg gtagtactgg ccttcgaagc tcacccggtc ctccgcccac aatttgcgga
      781 tcagcccgat ggcctcacgc atgtgggcga agcgctcctt catctccggc cacttgatgc
      841 cgatcgccgg cacttcgttc agactctccc ctgtgccgac acccaggacc acgcggcccg
      901 gaaacatctc gcccagagtc ccgaacacct gcgcgacgac ggacggatga tagcggaagg
      961 tcggcgtcag cacgctggtg cccatgacga tgcggctggt gctggcgccg agggcgccca
     1021 gccaggccag tgcggatggg gcatgaccgt cggtgtgctt ccacggctgg aaatggtcgc
     1081 tgacgaacac cgaatcgaac ccggcctgct ccgccgcaca ggagaacgcg agcagccgcc
     1141 tgggatcgaa ctgttcggcg gacgctttgt atccaagttt cagcacaaga cacctccggg
     1201 accattgggg cgcacgctag ccctgcggcc gggcagcggc aatcggccgc cccccgggcg
     1261 gcccaccccc ggccgtgcga tccgcggggc ggcccgggaa ctcacgcccg cagcccttgt
     1321 tggggtctgc gtcagccgaa cggcagcagg cgaagcggag accaacccat ggccgaaccg
     1381 gaacacaagg gcgtccaggt gcaggtggcg aatgcccgcc ccgaggacgt gggcaagggc
     1441 gtcgcccgcc tcagtcgcgg cgtgatggag acgatcggcg tgcgggaggg cgacgcgatc
     1501 gagattgccg gccaccgcca taccgcggcg ctggtgctgt cgccggcacc ggaggatgag
     1561 gggatcgaga tcatccggct cgacgggctg gaacgcgcca atgccggggt gtcggcgggc
     1621 gaacgggtgg aggtgcgccg cgcgccagtc cggccggccg gccgggtgac catcgcgccg
     1681 gcacagaaga acctgcgtct gtccgggtcg ggcgacgcgc tgcggcgcac gctgatcggc
     1741 cggccgctga tggccggcga cgtgatctcc acctcggtgt accagcggcc cgtcgccgcg
     1801 ggcgatggcg tgccggagga gctgatgcgg cacttcctgc agcagcagtc ctacgccctg
     1861 caggaaatcc ggctgacggt ggtgtccacc acgccgcgcg gcatcgtgcg catcgagccg
     1921 gacaccgaaa tcgagctgct gccggaatac gccgaaccga aggagacacg gcgcgccgac
     1981 gtcacctatg acgacatcgg cgggctgggc aacgcggtgg aacaggtgcg cgagatggtg
     2041 gagctgccgc tgcgccatcc ggagctgttc cagcggttgg ggatcgatcc gcccaagggg
     2101 gtgatcctgc atggcccgcc cggcaccggt aagacgctgc tggccaaggc ggtggcgaat
     2161 gaatccgaag cggcgttctt cagcatcgcc ggcccggaga tcatgggcag ccactacggc
     2221 gagtcggaac agcggctgcg cgacatcttc caggaagccg gccgccgctc ccccgccatc
     2281 atcttcatcg acgagctcga cagcatcgcc cccaagcggc aggaagcgcg cggcgaggtg
     2341 gaacgccgca tcgtcgccca gcttctgacg ctgatggacg ggctggaggc gcggcagaac
     2401 gtggtggtga tcgccgccac caaccgtgtc gatgcgctgg atgaggcatt gcgccggccc
     2461 ggccgcttcg accgggaaat cgtcatcggc gtgccggacc aggacgggcg gcggcaggtg
     2521 ctggcgatcc acacccgcgg catgccgctg cacgaggatg tcgatctcga cgggctggcc
     2581 cgcagcacat acggctttgt cggcgccgac gtcgcagccc tggcgcgcga ggcggcgatg
     2641 gacgcggtcc gccgcgtgct gccgcagatc aacctgcgcg acggcatccc ggccgaactg
     2701 ctggccggca tgcgggtctg ccgggaggat ttcgacaacg cgctgaagcg ggtgcagccc
     2761 tccgcgctgc gggagatcat gatccaggtg cccaacgtca cctgggagga tgtcggcggc
     2821 ctccaggaga cgcgcgagca gttgcgcgac ggcatcgagc tgccgctgaa gaacccggaa
     2881 gcgttccgcc gcctgggcat ccgcccggcc aagggcttcc tgctgttcgg gccgcccggc
     2941 accggcaaga cgctgatggc caaggcggtg gcgcgcgagg cgaatgccaa cttcgtcgcc
     3001 accaagtcct ccgacctgtt gtcgaaatgg tacggcgagt cggagcagca gatcagccgc
     3061 ctgttcgccc gggcacgcca ggtcgccccg acggtgatct tcatcgacga gatcgactcc
     3121 ctggtgccgg cccgtggcgg cgggctcggc gagccggcgg tcaccgaacg ggtggtgaac
     3181 acgatcctgg cggaaatgga tgggctggaa gacctgcaag gcgttgtggt gatcggcgcc
     3241 accaatcgcc cgaacctgct ggatccggcc ctgctgcggc cggggcggtt cgacgaactg
     3301 gtctatgtcc cggtgccgga cgaggccggg cggctgcaca tcctgcagat ccagaccagc
     3361 ggcatgccgc tggccgagga tgtcgatctg aagcggatcg ccgaacgcag caagggctat
     3421 accggtgccg atctcggaga cgtggtccgc cgcgccggcc tgctggcgct gcgtgagaac
     3481 ctgcagtcgg acatggtaca cgcggcgcag ttcgagcagg cgatgcgcgc gacgcgtgcc
     3541 tccgtcaccc cggaaatgga gcgggaatat gaggacttgc ggggccatct gaaggagcag
     3601 ggccccacgt cccgccagcc gatcggcttc gccatgggca ccggatgagg gaagaaatcc
     3661 ctcattgcgg agcgccaaag cggcgcctgc ttgaccttgc ttgagccgga cctcatgttc
     3721 cgcccgggtc gactcgtgaa tgagggctga tgtcgaaaga agacatgatc gaattcagcg
     3781 gcaccgtgat ggagctgctg ccgaacgcga tgttccgcgt gaagctggac aacgaacaca
     3841 ccatcctggc gcataccagc ggcaagatgc ggaagaaccg catccgcgtg ctggcgggcg
     3901 accgggtcaa tgtcgagatg acgccctacg acctgagcaa ggggcgcatc accttccgct
     3961 tcaaatagac actccacaac cgatgcagat gcccccgctg gtgctggcct cggccagtcc
     4021 gcggcgcttg gcgttgctgg cgcagatcgg cgtgacgccg gcgcgcgtgc tcccccctga
     4081 catcgacgaa actccccagc gggacgagct gccacggctg tacgcgcagc gcctcgcgcg
     4141 tgccaaggcg gcggccgtgg cagcgcccgg ctgcgcggtg ctggcggccg acaccgtggt
     4201 cgccgcgggg cgccgcatcc tgcccaaggc ggacaccgcg gcgcaggcgc ggcgctgcct
     4261 ggcgctgctg tccggccgac gccaccaggt catgaccgcc gtggtgctcg gcatgccgga
     4321 cggacgcagc agcgaacgcc tcgccaccag catcgtgagc ttcgcgcgcc tgaccgagcc
     4381 gcaga
//
LOCUS       DASSZH010000131         3013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_37294, whole genome shotgun sequence.
ACCESSION   DASSZH010000131 DASSZH010000000
VERSION     DASSZH010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37294"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1050)
                     /locus_tag="VFN42_02795"
                     /pseudo
     CDS             complement(169..1050)
                     /locus_tag="VFN42_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009797950.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            1266..2642
                     /locus_tag="VFN42_02800"
     CDS             1266..2642
                     /locus_tag="VFN42_02800"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HET8995573.1"
                     /translation="MSTDLDLSKLTHAEKDALILTLLARLDEASKLIAELRARIDDLT
                     RPGKTPDNSSVPPSKGQKPNRPDKARRGGPRKGSLGRTGGGRALTVAPDEVVVARPTR
                     CRHCQTAFSDADQTLDARYDKIDLPQVRPVVTRVERYAGHCRCCGGTTLAAVPEGLEP
                     GTPFSRNIVALAMYLRFVHAVSYKRLSRLMLDLFGLSISEGALDAAFRRGKKLFDADV
                     AAILGRLRRARVICSDETGVRIDGHGCWNWVFQNAEVVIHVVRRSRGADVVAEVLAGH
                     RPALWVSDLYGAQRGHAEHWQLCLAHQLRDCQYAIDAGDTVFAPRMKALLLRAVVLAR
                     RHRDLAESTRREYRRRLERDLDRVMSLAPPQRDGRRLRRRYARVRDHLFTFLDHPEIA
                     ADNNGRERELRPTATYRKVTGGFRSDWGADLFAAVRSVIGTAARRGIDAYQAIRDTLR
                     GQSTLAPG"
     gene            2778..>3013
                     /locus_tag="VFN42_02805"
     CDS             2778..>3013
                     /locus_tag="VFN42_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639391.1"
                     /note="type IV secretion VirD4 coupling protein family;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HET8995574.1"
                     /translation="MTGTKILWGQMLGALAMVLLGCWAATEWTAWRLGFQPALGLPWV
                     RIGAAPIYPPQLFFAWWYHFDAYAPGVFARGAAIA"
BASE COUNT          466 a         1013 c         1023 g          511 t
ORIGIN      
        1 aaggaggacg tcgcggcttg accctaacag cccctcgatg acagagagca gccagcccac
       61 gggcagcgcc acccccggcc gacatccgtc ggaatgaccg gccgacttca atcggaacag
      121 gtggccgaca tcacgccgga atcaccggcc gacttcgtcg gaatccgcat cgatggccga
      181 gcgccgcccg ttgtcgttct gcgccacgtg cggggtgacg ttgatctcgc gcatctcggc
      241 gacgaagtcc tgcgtgtcga agccctagcc gccgcccacc ttgatgcgct gggtgcggtc
      301 ggcgtgcggt tcgatcaggt gctgcgcggc cagccgttcg gcatgccccg aggcgcgggt
      361 ggcggtggcg cctacgatca ggccgttacg gttctccatg agcgcgtgcc ccataaaaca
      421 gagcttagcc tccttgcccc gcccctttcg gtagagccgg gcatccgggt cggtgatgga
      481 agcgtgggtc tcgttgctgc gcttctggcc gtggaaatcc tgctcgccgt tgcgccccgg
      541 atcgggcggc gggccggatc cgtccttcgg ccgaaagctc ttgatgctcg cccatgcttc
      601 cagcagcgtg ccgtccaccg agaaatgctc gtccgacagc agcaccttga cctgcggttg
      661 cgccagcacg gcggcgagga acttggtcgc gatgtcgccg tccagcagcc ggtcgcggtt
      721 cttggtgaag gtggtcgcgt cccacaccgg gtcgtccatg cccagcccga cgaaccagcg
      781 gaacagcagg tcgaagtcga tgcgctcgac caactgccgc tccgagcgga tggtgtagaa
      841 ggcttgcagc agcagcgccc gcagcagccg ctccggcggg atggattccc gcccgaacgg
      901 cgaatagagc gcgtcgaact ccgccgacat cgccaccagc gtcgcgttca cgatctcccg
      961 gatcacccgc agcgggtgat cggcgggcac ccgcttctca aggttcacgt agctgaacag
     1021 cttgccgctg accgcatcgc tcccgcgcat tcctgccccg cgcacccgct gacagggtca
     1081 cagggaatcg caattcgatc cgcgccgcca gtggcgaatt tcagcagcct gttagaagta
     1141 tagccgctcc ggattgagga tgagtaactg ctcaacccgg tctgctggcg tctgtggcgg
     1201 ggttgggtga ggtgacggag gcaagggggt tgacgcggta acgggcgact cgattcaagg
     1261 ttggggtgag caccgacctt gatctgagca agctgacgca cgcggagaag gacgcgctga
     1321 tcctgaccct gttggcgcgg ctggatgaag cgtccaagct gatcgcggag ttgcgagcgc
     1381 ggatcgacga cctcacccgg ccgggcaaga cgccggacaa ctccagcgtg ccgccgtcga
     1441 aggggcagaa gccgaaccgg ccggacaagg ccaggcgtgg cggtccgcgc aaaggcagtc
     1501 tcggccgcac gggcggcggg cgggcgctga ccgtggcgcc ggatgaggtg gtggtggcca
     1561 ggccgacgcg ctgccgacac tgccaaacgg cattttccga cgccgaccag acgctcgacg
     1621 cccgctacga caagatcgac ctgccgcagg tgcgtcccgt cgtgacccgg gtggagcggt
     1681 atgccgggca ctgccgctgc tgcggcggca ccacgctggc ggcggtgccg gagggattgg
     1741 agccgggcac gccgttcagc cgcaatatcg tggcactggc gatgtatctg cgcttcgtgc
     1801 atgcggtcag ctacaagcgg ctgagccggc tgatgcttga tctgttcggc ctgtcgatca
     1861 gcgaaggcgc gctggacgcc gcgttccgcc gcggcaagaa actgttcgac gccgatgtcg
     1921 cggcgattct gggccgcctg cgccgggcgc gggtgatctg ctcggacgaa accggcgtgc
     1981 gcatcgacgg tcatggctgc tggaactggg tgtttcagaa cgccgaggtg gtgatccacg
     2041 tcgtgcgccg cagccgtggc gccgacgtgg tggccgaggt gctggcaggc caccgcccgg
     2101 cgctgtgggt ttccgacctt tatggcgcgc agcggggcca cgccgagcac tggcagctct
     2161 gcctcgcaca ccagttgcgt gactgccagt atgccatcga cgcgggcgac acggtgttcg
     2221 ccccgcgcat gaaggcgctg ctgctgcgtg ccgtggtgct ggcgcggcgc catcgcgatc
     2281 tggccgagag cacgcggcgc gaataccgac ggcgactgga acgcgacctc gaccgcgtga
     2341 tgtcgctggc accgccgcag cgcgacggcc gccgcctgcg caggcgatac gcccgcgtcc
     2401 gcgatcacct gttcaccttc ctcgatcatc ccgagatcgc cgccgacaac aacggccgcg
     2461 agcgcgaact gcggcccacc gcgacatacc gcaaggtcac cggcggcttc cgctccgact
     2521 ggggcgccga cctgttcgcc gccgtccgct ccgtcatcgg aaccgccgcg cgccgcggca
     2581 tcgacgccta tcaggcaatc cgtgacaccc tacgcggtca atccaccctc gcaccgggtt
     2641 gagcagttac gaacgagggg cgcaacccgc cgttcatttc tattctttcg atcctctacg
     2701 attgccgttc tttgctgttg cgcggcggag tttccgtctt tctatctgtg tcccgttcgc
     2761 atccatgagc ggggtcgatg accgggacga agattctctg gggccagatg ctgggcgcac
     2821 tcgccatggt gctgctcggc tgctgggccg ccacggaatg gacggcctgg cggctcgggt
     2881 tccagccggc gctcggcttg ccctgggtcc gaattggcgc ggcgccgatc tatccgccgc
     2941 agctcttctt cgcgtggtgg taccatttcg acgcctatgc tcccggcgtc ttcgccaggg
     3001 gtgcggcgat cgc
//
LOCUS       DASSZH010000132         2244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_889720, whole genome shotgun sequence.
ACCESSION   DASSZH010000132 DASSZH010000000
VERSION     DASSZH010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889720"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VFN42_02810"
     CDS             <1..390
                     /locus_tag="VFN42_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995575.1"
                     /translation="GEDWPKLVWILDTRLESNLVCIGTLPLPPVEEHAYRGGRYGAHN
                     MHENRPGPAFRSSTLVFGTYFNGGIRVHDISNPFQPKEVAYYVPEPPEGSRVPSVQIN
                     DVYVDENRVVYAVDRFAGGLYTLEMTV"
     gene            complement(438..1064)
                     /locus_tag="VFN42_02815"
     CDS             complement(438..1064)
                     /locus_tag="VFN42_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995576.1"
                     /translation="MRVLFGMDRLPRSLRPDDPRLPSVTLTAAPLRLNSEPLPPETVA
                     AGQGGHAARIAASEAWLTAACGSYAPLRQRFIAFWLRFAGAHVAAHRAELQEKLRPFD
                     GLYAPEDFVWSALRPLPRALLPSGDGVVPVDAAFWDGTRLTAVLLGDAPDVAIDGVGV
                     CRLRAADLDSDGPALVAQRLPAGFLRFWLGETLPMTPFRRALPESQAV"
     gene            complement(1085..>2244)
                     /gene="pcaF"
                     /locus_tag="VFN42_02820"
     CDS             complement(1085..>2244)
                     /gene="pcaF"
                     /locus_tag="VFN42_02820"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HET8995577.1"
                     /translation="RYAGALKDVRTDDLAAHPLKTLMARHPDLDWEAVEDVIMGCANQ
                     AGEDNRNVARMALLLAGLPSSIPGATVNRLCGSGLDAVGTAARAIKTGEAELMIAGGV
                     ESMTRAPFVMGKATEAFSRSSEVYDTTIGWRFVNAQMKKVYGTDSMPETGENVAEEYQ
                     ISRQDQDAFAYRSQQRAKAAQDSGFFAKEIAPVTIKGRKGDTVVDKDEHPRPDTTMEM
                     LAKLKTPFRDPGTVTAGNASGVNDGAAALIIASEDAARRHGLTPRARVIAMATAGVQP
                     RVMGIGPAPAVRKLLAKTGLSLDEIDVIELNEAFAAQGLAVLRQLGLPDDADHVNPHG
                     GAIALGHPLGMSGARLALTAVNALEESGRKRAIATMCIGVGQGIAALIERV"
BASE COUNT          337 a          771 c          787 g          349 t
ORIGIN      
        1 ggcgaggact ggccgaagct ggtctggatc ctggacaccc ggctggagag caatctggtg
       61 tgcatcggca cgctgccgct gccgccggtg gaggagcacg cctatcgcgg tggccgctac
      121 ggcgcgcaca acatgcacga gaaccggccg ggaccggctt tccgttccag cacgctggtg
      181 ttcggcacct acttcaatgg cgggatccgg gtgcacgata tcagcaatcc attccagccg
      241 aaggaagtgg cgtactacgt gccggagccg ccggagggtt cgcgcgtgcc gtccgtgcag
      301 atcaacgacg tgtacgtgga tgagaaccgg gtggtctatg cggtcgatcg ctttgccggc
      361 gggttgtaca cgttggagat gacggtatag ccaaatctcc acccaacctg ttccacaagg
      421 ggaggaggaa tagtcttcta caccgcctgc gactctggca gcgctcggcg gaacggtgtc
      481 atcggcaagg tttctcctag ccagaaccgc aggaatccgg ctggcagacg ctgcgcgacg
      541 agcgccggtc cgtcgctgtc cagatccgcc gcgcgcagcc ggcagacgcc gacgccgtca
      601 atcgcgacat ccggtgcgtc gcccagcagc accgcggtca gccgcgtgcc gtcccagaac
      661 gccgcatcca ccggcacgac tccatcaccc gacggcagca gcgcacgcgg caacggacgc
      721 agtgcggacc agacgaaatc ctccggcgca tagaggccat cgaacgggcg cagcttctcc
      781 tgcagctccg cccggtgcgc ggcgacatgc gccccggcaa accgcagcca gaacgcgatg
      841 aaacgctgcc gcagcggtgc ataggacccg caggcggcgg tcagccaggc ctcggacgcg
      901 gcgatccgcg ccgcatgtcc gccctgtccc gcggcgaccg tttccggcgg caacggttcg
      961 ctgttcagcc gcagcggtgc cgcggtcagc gtgacggagg gcaggcgcgg atcgtccggc
     1021 cgcaggctcc gcggcaaccg gtccatgccg aacaggacgc gcatcgccgc ggatggctcc
     1081 gcgctcagac ccgctcgatc agcgccgcga tgccctggcc gacgccgatg cacatggtgg
     1141 cgatcgcgcg cttgcggccg ctctcctcaa gcgcgttcac cgcggtcagg gccaggcggg
     1201 cgccgctcat gcccagcggg tgcccgagcg cgatggcgcc gccatgcggg ttcacgtggt
     1261 cggcgtcgtc gggcaggccg agctgacgca gcacggcgag gccttgcgcg gcgaaggcct
     1321 cgttcagctc gatcacgtcg atctcgtcca ggctcagccc ggtcttcgcc agcagcttgc
     1381 gcacggcggg cgccgggccg atgcccatca cgcgcggctg cacgccggcg gtggccatgg
     1441 caatgacgcg ggcacgtggc gtcaggccgt ggcggcgggc ggcgtcctcc gacgcgatga
     1501 tcagcgcggc agcgccgtca ttcacgccgg acgcgttgcc ggcggtgacg gtgccggggt
     1561 cgcggaacgg cgtcttcagc ttggccagca tctccatcgt ggtgtcgggg cgcggatgct
     1621 cgtccttgtc caccaccgta tcgcccttgc gccccttgat ggtgacgggc gcgatctcct
     1681 tggcgaagaa gccggagtcc tgcgcagcct tggcgcgctg ctggctgcga taggcgaacg
     1741 cgtcctggtc ctggcgggag atctggtact cctccgccac gttctcgccg gtctccggca
     1801 tggaatccgt gccgtagacc ttcttcatct gcgcgttgac gaagcgccag ccgatggtgg
     1861 tgtcgtagac ctccgaggaa cgcgagaatg cttcggtggc cttgcccatg acgaaggggg
     1921 cgcgggtcat gctttccacg ccgccggcga tcatcagttc ggcctctccg gtcttgatgg
     1981 cgcgggcagc ggtgccgacc gcgtccaggc cggagccgca caggcggttc accgtggcgc
     2041 cgggaatcga cgagggcaag ccggccagca gcagcgccat gcgggcgacg ttgcggttgt
     2101 cctcgccggc ctggttggcg cagcccatga tgacgtcttc caccgcctcc cagtccaggt
     2161 ccgggtggcg cgccatcagc gtcttcagcg gatgggcggc gaggtcgtcg gtgcgcacgt
     2221 ccttcagggc gccggcgtag cggc
//
LOCUS       DASSZH010000133         2097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1043207, whole genome shotgun sequence.
ACCESSION   DASSZH010000133 DASSZH010000000
VERSION     DASSZH010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043207"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1401)
                     /locus_tag="VFN42_02825"
     CDS             complement(16..1401)
                     /locus_tag="VFN42_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HET8995578.1"
                     /translation="MPTECIPEQFEFPRLEGRAVVAAFDGGRITTDAGALLLGAADRV
                     IGLTRRFAACFKDARHAPFVEHSVQTLVTQRVVGIALGYEDLIDHDELRHDPVLAVLA
                     GKLSAGRSDCAPLAGKSTLNRLERSRSEPSRYARIAADPEAIEALLVDLFLDAHATAP
                     EQITLDLDATDDPLHGHQEGRFFHGYYDSYCYLPLYIFCGRHLLAAKLRPANIDAAAG
                     AREEIARLVGQIRGRWPQVRILLRADSGFCRDELMTWAEENRVDYVFGLARNARLVEE
                     ITAELQTARAISEASGKPARRFKDFRWRTRKSWSCERRVIGKAEWTQNEANPRFVVTS
                     LAPAEVEDGAKYLYERIYCARGEMENRIKECQLDLFADRTSSATMRANQLRLWFSSLA
                     YVLMCALRRIGLAETEFATATCGTIRLKLLKIGALVRISVRRVSIAMASACPWRGVFA
                     HAHTALQRAAG"
     gene            1552..>2097
                     /locus_tag="VFN42_02830"
     CDS             1552..>2097
                     /locus_tag="VFN42_02830"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HET8995579.1"
                     /translation="MPNVTADRLRAIAQALLEGAGTAPEEAATVARLSIAANLAGHDS
                     HGIILIPTYIDRVKKGHIVPGAKWEIVQETPTTTVVDGHWGFGYTVTERAMRHTIAKA
                     KTQNVAATTVFRQGHIGRLAAYPLMAAEEGLIAMITADSGRSPKHVAPFGGAKARLGT
                     NPICFAVPSELDGPLFFDMATS"
BASE COUNT          350 a          731 c          681 g          335 t
ORIGIN      
        1 ctggttcgtg tccgctcagc cggcggctcg ttgcaacgcg gtgtgggcgt gcgcgaagac
       61 gccccgccac gggcaggcgg acgccatggc gatgctcacc cggcgcacac tgatccgcac
      121 cagcgcgccg atcttcagca gtttcagccg gatcgtgccg catgtggcgg tggcgaattc
      181 cgtctccgcc agcccgatcc ggcgcagcgc gcacatcagc acgtaggcca gcgaggagaa
      241 ccacaggcgt aactgattgg cgcgcatggt tgccgaggaa gtgcgatcgg cgaacaggtc
      301 caactggcat tccttgatcc gattctccat ctccccgcgc gcacaataga tccgctcgta
      361 gagatatttt gctccgtcct cgacctccgc cggcgccagc gaggtgacga caaaacgcgg
      421 gttcgcctca ttctgggtcc attccgcctt gccgatcacg cgccgctcgc agctccaact
      481 cttgcgcgtg cgccaacgga agtccttgaa ccgccgcgcc ggcttgccgc tcgcctcgga
      541 gatcgcccgc gcggtttgca gctcggccgt gatctcttcc accagacgcg cgttgcgcgc
      601 caggccgaac acgtaatcca cccggttctc ctcggcccag gtcatcagct cgtcgcggca
      661 gaagccgctg tcggcgcgca gcaggatgcg cacctggggc cagcggccgc ggatctggcc
      721 gacgagacgc gcgatctcct ccctcgcccc ggccgcggcg tcgatgttcg ccggccgcag
      781 cttcgccgcc agcagatgcc ggccgcagaa gatgtacagc ggcaggtagc agtagctgtc
      841 gtaatagccg tggaagaacc ggccctcctg gtgcccgtga agcggatcgt cggtggcgtc
      901 cagatcgagc gtgatctgct ccggggccgt cgcgtgggcg tccagaaaca gatccaccag
      961 cagggcttcg atcgcctccg gatccgcggc gatgcgggcg tagcggctgg gttcggagcg
     1021 gcttcgttcc aaccggttca gcgtgctctt gccggccagc ggcgcacagt ccgaccgccc
     1081 ggcggaaagc ttgcccgcca gcaccgccag caccgggtca tggcggagtt cgtcgtgatc
     1141 gatcaggtcc tcgtacccca gcgcgatgcc gaccacgcgc tgcgtcacca gcgtctgtac
     1201 gctgtgttcc acgaacggcg catggcgcgc gtccttgaag caggcggcaa agcgccgggt
     1261 cagaccaatc acccggtcgg ccgcgcccag caacagcgcg ccagcgtcgg tcgtgatgcg
     1321 cccaccatcg aacgccgcta ccacggcgcg gccttccaga cgtggaaatt caaactgctc
     1381 cgggatacac tctgtcggca tcggggacct cctctgcttc gccgcaagac tttgtcgcag
     1441 aagaactttc gccgattcag agccccgatg cacctgcttt ctgtgagaaa tgcgggctag
     1501 gcgcggcggc tccggttggg aatactgccg cccgtaccga gggaggatgc catgccgaac
     1561 gtcaccgccg accgcctgcg cgcgatcgcc caagccctgc tggagggcgc cggcacggcg
     1621 ccggaggaag ccgccacggt ggcccgcctg tcgatcgcgg ccaatctggc cgggcacgac
     1681 agccacggga tcattcttat cccgacctat atcgaccggg tgaagaaggg ccatatcgtc
     1741 cccggcgcga aatgggagat cgtgcaggag acgccgacca ccaccgtggt cgatggccac
     1801 tggggcttcg gctacacggt gaccgaacgc gcgatgcgcc acacgatcgc gaaggcgaag
     1861 acgcagaacg tcgcggcgac gacggtgttt cgccagggcc atatcggccg gctcgcagct
     1921 tatccgctga tggcggccga ggaagggctg atcgcgatga tcaccgccga ttccggacgc
     1981 agcccgaagc acgtcgcccc gttcgggggg gcgaaggcgc ggttggggac caacccgatc
     2041 tgcttcgcgg tgccgtccga actggacggc ccgctgttct tcgacatggc cacgtcc
//
LOCUS       DASSZH010000134         3614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1196473, whole genome shotgun sequence.
ACCESSION   DASSZH010000134 DASSZH010000000
VERSION     DASSZH010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196473"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="VFN42_02835"
     CDS             <1..902
                     /locus_tag="VFN42_02835"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET8995580.1"
                     /translation="LSLATRASAFQEIARTRITQGISGPVSQIILGLLGFGAPALVIG
                     FIIGQSSGTLLLLRRWVLARPEWLRSVSWCGMRAAARRYAQFPLFASWSRLLESAGSG
                     LVLFLIFSACFSSQVAGFMFLSERVIWRPLLLVSSSLLQVFTGEAGRAVSQDPERLRR
                     RFYQVVPRQFLLVAAWVALANLAAGWAFPVLFGAAWGDAIPFLRALSVSYLMQAVLHP
                     LSTTMQILEYQVTAVLWQIGRLTLLVGGVLLAWHAGLSALTALWIASLIQAACCLVLL
                     GLMAWAIEQVVARQPKPEMALGE"
     gene            993..2261
                     /locus_tag="VFN42_02840"
     CDS             993..2261
                     /locus_tag="VFN42_02840"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007006172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HET8995581.1"
                     /translation="MSARIDGIVARLHGLHPRLIDLSLDRLLVLLARLGHPERRLPPV
                     IHVAGTNGKGSTCAFLRAIAEAAGLRVHVYTSPHLVRFNERIRIAGALVTDDALVAAM
                     EQVEQANAGSPITVFEVITAVAFHLFAQTPADLCVLEVGLGGRGDATNVIPPPAACAI
                     TSISLDHRELLGDTLGAIAAEKAGIMKPGVPVATGSQAPEADAVLRAEAARIGAPLLR
                     RGHDWHVQPHGGGFRYADAHGALTLPAPSLPGAHQWDNAGIAIAALRTAGLGISEAAI
                     ADGVTRATWPARMQRLHGALARLLPPDWELWLDGGHNPGAGAALGAFLAGWADRPVHL
                     VVGMKQAKDSAEFLRPLLPHASTVWAVAEPDQHLAMPVEAIIAASGGVARPGPLVGDA
                     LRSLAHEAGPARVLICGSLYLAGEVLKQDG"
     gene            2353..3309
                     /locus_tag="VFN42_02845"
     CDS             2353..3309
                     /locus_tag="VFN42_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HET8995582.1"
                     /translation="MATHRIAPTPQTVRWGTFDASYPPLITVESGDTVVLECVSGGPE
                     VMPPAGSGFSIPPALATIHGANLPRAGGHILTGPVGVAGADPGDMLEVRIDRIELGND
                     WGYCGFRPLAGTLPEDFPDRFLSHIPVDRTRKVCTLPWGTELALAPFFGVMGVAPPAR
                     YGTISTIQPREHGGNLDNKELTEGSTLFLPVWERGALFSAGDGHGVQGDGEVCINALE
                     ICLTGTFTLVLHKGRGAHDPLLKYPRAETPTHFISMGMHEDLDVAMKTALREMIAFIA
                     ARTNLSREQAYQFCSLAVDFHVTQTVNGEKGVHGMLRKGLLF"
     gene            3431..>3614
                     /locus_tag="VFN42_02850"
     CDS             3431..>3614
                     /locus_tag="VFN42_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HET8995583.1"
                     /translation="MEWADETITRLRELWSEGHSTAEIGRRLGVSKNAVVGKAHRLDL
                     PARPSPIRREGDALPPR"
BASE COUNT          497 a         1254 c         1236 g          627 t
ORIGIN      
        1 tgctgtcact ggcaacccgc gcgagcgcgt tccaggagat tgcccgcacc cgcatcaccc
       61 agggcatctc cggacccgta tcgcagatca tccttggact gctcggcttc ggcgcaccgg
      121 ccctggtgat cggcttcatc atcggccagt ccagcggcac actcctgctg ctgcgccgct
      181 gggtgctggc ccggcccgag tggctgcgca gcgtttcctg gtgtgggatg cgtgccgcgg
      241 cccggcgcta tgcgcaattc ccgctatttg ccagttggtc gcgcctgctg gagtcggccg
      301 gcagcggatt ggtgctgttc ctgatcttct cggcatgctt ttccagccag gtcgccggct
      361 tcatgttcct gtcggagcgg gtgatctggc gtccgctgct gctggtcagc agttcgttgc
      421 tgcaggtgtt caccggcgag gccggacgcg ccgtctcgca ggatccggaa cgcctgcgcc
      481 ggcgattcta ccaggtcgtg ccgcggcagt tcctgctggt ggcggcctgg gtggcgctgg
      541 ccaatctggc ggccggctgg gctttcccgg tgctgttcgg cgccgcctgg ggcgatgcca
      601 ttcccttcct gcgcgcgttg agcgtgtcgt acctgatgca ggccgtgctg cacccgctct
      661 ccaccacgat gcagatcctg gaataccagg tcaccgccgt gctgtggcag atcggccggc
      721 tgaccctgct ggtcggcggc gtgctgctgg cctggcacgc cggcctgtcg gcgctgaccg
      781 cgctgtggat cgcgtcgctg attcaggccg cctgctgcct ggtgctgctc gggctgatgg
      841 cctgggcaat cgagcaggtc gtcgcccgcc agcccaaacc ggagatggcg ctcggcgaat
      901 agcatgacgt cggggatgca gttctcccct tcgccggccc acccgacagg ggcatcttcg
      961 ctgccgcaca cgcgccgtga taaggccggc gcatgagcgc acgcatcgac ggcatcgtcg
     1021 cccgcctcca cggcctgcac ccgcgactga tcgacctgag cctggaccgg ctgctggtac
     1081 tcctggccag gctcggtcac ccggaacgcc gcctgccgcc ggtgatccat gtcgccggca
     1141 ccaacggcaa aggcagcacc tgcgccttcc tgcgcgccat cgccgaggcc gccgggctgc
     1201 gggtgcacgt ctatacctcg ccgcatctgg tgcgtttcaa cgaacgcatc cgcatcgccg
     1261 gtgccttggt gacggacgac gcgctggtcg ccgcgatgga acaggtggaa caggcgaatg
     1321 ccgggtcgcc gatcaccgtg ttcgaggtga tcaccgcggt cgccttccac ttgtttgccc
     1381 agacgccggc cgatctctgc gtgttggagg tcgggctggg cggccgtggc gacgccacca
     1441 atgtcattcc tccaccggct gcctgcgcca tcacctccat ctcgctggac catcgggaac
     1501 tgctgggcga cacgctgggg gcgatcgcgg cggagaaggc cggcatcatg aagcccggcg
     1561 tgccggtggc gaccggcagc caagcgccgg aagccgacgc cgtgctgcgt gccgaggccg
     1621 cccggatcgg tgccccccta ctgcggcgcg gccatgactg gcacgtgcag ccgcacggag
     1681 gcggattccg ctacgcggat gcgcacggcg ccctgacact gccagcaccg tcgctgcccg
     1741 gggcgcacca gtgggataat gccggcatcg cgattgccgc gttgcgcact gccggccttg
     1801 gcatttccga ggcggccatt gccgacgggg tcacccgggc gacgtggccg gcccgcatgc
     1861 agcgcctgca cggagcgctc gcccgcctgc tgccaccgga ttgggagctg tggctcgacg
     1921 gcgggcacaa tcccggtgcc ggcgcggctc tgggtgcgtt tcttgccggc tgggcagacc
     1981 ggccggtgca tctggtggtc ggcatgaagc aggcgaagga ttccgcggaa ttcctgcggc
     2041 cgctgctgcc gcatgcaagc accgtctggg cggtcgcgga gccggaccag cacctggcca
     2101 tgccagtaga ggcgatcatc gccgcgtccg gcggggttgc gcgcccgggt cccttggttg
     2161 gcgacgcgct gcgatccctc gcgcacgaag ccggcccggc gcgcgtgctg atctgcggca
     2221 gcctgtacct cgccggcgaa gtgctgaagc aggacggctg agcgccgaca ggacgcgcgg
     2281 aatcaccgtt gccagcagcg tgcttccggg cgacgttgca cagcagcacg tcctggcatc
     2341 ccgaggttcc gcatggccac gcaccgcatc gcccccaccc cgcaaaccgt ccgctggggc
     2401 acgttcgacg cgtcttaccc gccgctgatc accgtcgagt caggcgacac cgtggtgttg
     2461 gaatgcgtct ccggcgggcc ggaggtgatg ccgcccgctg gctccggatt cagcatccct
     2521 ccggcgctgg ccaccatcca cggtgccaac ctgccgcgtg ccggcggcca tatcctgacc
     2581 ggaccggtcg gggtggccgg cgccgacccc ggcgacatgc tggaagtccg catcgaccgt
     2641 atcgagctcg gcaatgactg gggttattgc ggcttccgcc cgctggccgg cacgctgccg
     2701 gaggattttc ccgaccgctt cctcagccac atcccggtgg accggacgcg caaggtctgc
     2761 accctgccct ggggcacgga actggctctg gcgccgttct tcggcgtgat gggcgtcgcc
     2821 ccgccggccc gctacggcac catctccacc atccagccgc gcgagcacgg cggcaacctg
     2881 gacaacaagg aactcaccga gggcagcacg ctgttcctgc cggtgtggga gcgcggggcg
     2941 ctgttctccg cgggcgacgg gcacggcgtg cagggcgacg gcgaggtgtg catcaatgcg
     3001 ctggagatct gcctgaccgg caccttcacc ctggtgctgc acaagggccg cggcgcgcat
     3061 gacccgctgc tgaaatatcc ccgcgcggag acgccgaccc acttcatctc gatgggcatg
     3121 catgaggatc tggacgtggc catgaaaacc gcgctgcgcg agatgatcgc cttcatcgcc
     3181 gcccgcacca acctgtcgcg tgagcaggcc taccagttct gctcgctcgc ggtggatttc
     3241 cacgttacgc aaacggtaaa tggggagaag ggcgtgcacg ggatgttgcg aaagggcttg
     3301 cttttctagt gcttgtggtt gtcctctaag ttgtccacaa ctttttgggg ttccgcgccg
     3361 attcgcggtt gagccgccgc tcccaggtct ataccgtatc ttgtggttgg acctgcggag
     3421 ggaccaagat atggagtggg cggacgaaac catcacgcgg ctgcgggagc tgtggtcgga
     3481 aggccattcc actgccgaaa tcggccgccg gctgggcgtg tcgaagaatg ccgtggtcgg
     3541 caaggcgcac cggctggacc tgccggctcg gccgtcgccc atccgccgcg agggcgatgc
     3601 gctgccgccg cgcc
//
LOCUS       DASSZH010000135         4084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_463532, whole genome shotgun sequence.
ACCESSION   DASSZH010000135 DASSZH010000000
VERSION     DASSZH010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463532"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VFN42_02855"
     CDS             <1..191
                     /locus_tag="VFN42_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995584.1"
                     /translation="ASTGVIEWISAGVSVVIVVLMVAWYIRFRRHEDAMEATADAALP
                     GPLRPRHDDPHALQSEGR"
     gene            complement(228..1082)
                     /locus_tag="VFN42_02860"
     CDS             complement(228..1082)
                     /locus_tag="VFN42_02860"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8995585.1"
                     /translation="MSQDRNTGGKPVAVVVGATSKWQSDGRNTRLAHGAPVDDSAVPV
                     AARWGVGGAIAQKFAREGYFVVLTTRNAANAEALAAAIRAQQGACSIVPLDLASQDSV
                     TAAFAAIRRDAGDPDVLVYNAGYLEGRDLPPGQELLEYIPAAMFDATQHIACRGPFLV
                     AKEVLPAMRQKRAGSFFFSNNAKSLRGRKRLTGESLYYPRVMMRALAQVLTEEYSEHG
                     VHVANVVIDGTIDSPGTRALPKLQNRPELAINPAAIADAFWYLHTQDRSCWTHELQLT
                     PYATVPSY"
     gene            complement(1121..1948)
                     /locus_tag="VFN42_02865"
     CDS             complement(1121..1948)
                     /locus_tag="VFN42_02865"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8995586.1"
                     /translation="MQIIDAQIHLWGSGLPSNLSHRQVTHFTAEEAIPLMDEGGVDAA
                     VIHPPGWDPHATEMAFKAVHDYPGRFAILGALPLEDPESRTRIATWRQQPGMLGLRYT
                     FLHDPARQWLHDGTLDWLWAAAERAGVPIAMLATDSLADIARIAERHPGLRLTIDHLG
                     GRGGLTTLKDAAAMTHMPQLLALAKYPNVAVKATGAPGYSSEPYPFPTMQSYLRQIFD
                     AFGPQRMFWGTDITKMPCSWRQCVTMFTEELPWLTAQDKRLVMGDALCAWWGWDRAA"
     gene            complement(2169..3770)
                     /locus_tag="VFN42_02870"
     CDS             complement(2169..3770)
                     /locus_tag="VFN42_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HET8995587.1"
                     /translation="MESQTFDYIVVGAGSAGSVLANRLSADPRHKVLVLEAGRESHPW
                     SRIPVGFAKLIENPAANWLYSSEPDAGTGQRRIPIPRGKLLGGSSSINGMVFVRGQSQ
                     DYDTWAQLGNRGWSYREVLPIFKAMESFQGAADEEYRGRNGPLKVTESNESGPIYDAL
                     IQAAGAVGVPFTRDYNGAQQDGIAMTQATIRNGRRMSTAVCYLDPARGRPNLTIQANA
                     LTECLLLDGKRCTGVRYTVNGQQREARAAREVIVSAGSINSPQLLELSGIGQPELLQS
                     LGIDVRHALKGVGENLRDHYSPRMKWLVPPALGLTYNDKGRGLRMIWQALRYATASKG
                     LLGLPASPIRAYVRTRPGLDAPNAAIAWLPFLADANYKLAKRSGITAIVNILRSESTG
                     SIHITSASAQTPPAVRFNFLSAQLDRDVTLEVMRLTRRIMAAPPMDGIVREEIAPGAA
                     LQSDDEMLDWVRNHAETTYHPVGTCKMGHDPMAVVDDRLRVHGIQGLRVADASIMPTL
                     TSGNTNAPSIMIGEKAARMILADAG"
BASE COUNT          622 a         1389 c         1380 g          693 t
ORIGIN      
        1 acgcctcgac cggcgtgatc gaatggatct cggccggggt ctcggtggtg atcgtcgtcc
       61 tgatggtcgc ctggtacatc cgcttccgcc ggcatgagga cgcgatggag gccactgccg
      121 acgcggcgct gcccgggccg ctgcggccgc gccatgacga tccgcatgcc ctgcaatcgg
      181 agggtcggtg aggcggccgc tgaagcgggc cggcctgtcg catcggctca gtagctcggc
      241 accgtggcat agggcgtcag ctgcagttcg tgcgtccagc acgaccggtc ctgcgtgtgc
      301 aggtaccaga aggcgtcggc gatcgccgcc ggattgatcg ccagttccgg acggttctgg
      361 agcttcggca gtgcccgcgt gccgggcgag tcgatcgtgc cgtcgatcac cacattggcg
      421 acgtgcacgc cgtgttcgga gtattcctcg gtcagcacct gcgcgagcgc ccgcatcatc
      481 acccgcgggt agtacagcga ttccccggtc agccgcttgc gtccgcgcag cgacttggcg
      541 ttgttggaaa agaagaagga cccggcgcgc ttctgccgca tcgccggcag cacctccttc
      601 gccaccagga acggaccgcg gcaggcgatg tgctgggtgg cgtcgaacat cgccgccggg
      661 atgtattcca gcagttcctg cccgggcggc aggtcgcggc cttccaggta gcccgcgttg
      721 tagaccagca cgtcgggatc gcccgcatcg cggcggatcg ctgcgaaggc cgcggtgacc
      781 gagtcctgcg acgccaggtc cagcggcacg atgctgcagg caccctgctg cgcgcggatg
      841 gccgccgcca gtgcttcggc attggccgcg ttgcgggtgg tcagcacgac gaaatagcct
      901 tcacgggcga atttctgcgc gatggcaccg ccgacgcccc agcgcgcggc aaccggcacc
      961 gcgctatcat ccaccggcgc gccgtgcgcc agccgcgtgt tgcggccgtc cgactgccac
     1021 ttcgacgtgg cgccgaccac cacggcgacc ggcttgccgc cggtattgcg atcctggctc
     1081 atggcgcaga cgctccctcg gtattgctat gtggcgtgag tcaggcggcg cggtcccagc
     1141 cccaccaggc gcacagcgcg tcgcccatca ccagccgctt gtcctgcgcc gtgagccacg
     1201 gcagttcctc ggtgaacatc gtcacgcatt gccgccagga gcatggcatc ttggtgatgt
     1261 cggtgcccca gaacatgcgc tgcggcccga acgcgtcgaa gatctgccgc agatagctct
     1321 gcatcgtggg gaacgggtag ggctcgctgg aatagcccgg cgcaccggtc gccttcaccg
     1381 cgacgttggg atacttcgcc agcgccagca actgcggcat gtgcgtcatc gccgcggcat
     1441 ccttcagcgt ggtcagcccg ccacgcccgc ccaggtgatc gatggtcagc cgcaatcccg
     1501 gatgccgttc ggcgatgcgg gcaatgtcgg ccagcgaatc cgtcgccagc atcgcgatgg
     1561 gaacgccggc ccgctcggcc gccgcccaca gccagtcgag cgtgccgtca tgcagccatt
     1621 gccgcgccgg atcgtgcagg aaggtgtagc gcaggcccag catgccgggc tgctgccgcc
     1681 aggtcgcgat gcgggtccgg gactccggat cctcgagcgg caacgcaccc aggatggcga
     1741 agcggccggg atagtcgtgc accgccttga acgccatctc ggtggcgtgc gggtcccagc
     1801 cgggcggatg gatcactgcg gcatcgacgc cgccttcgtc catcagcggt atcgcctcct
     1861 cggcagtgaa atgcgtcacc tgccggtgcg acaggttgct tggcagcccg cttccccaga
     1921 gatggatctg tgcatcgatg atctgcacgg cgcttctctc ccatcaagca gaaacagcag
     1981 aaaacaggaa ccgcagatga acgccgatga acgcggatcg cgtggtgacg cccggcatgg
     2041 cgcgcgtact ccccgccgac gccagcaatg gctgtgcggc ggaacccaag gccgtgcgac
     2101 tccacgcgat cggctgaaga ccgtcggcgt tcatcggcat tcatctgcgg ttccatttgc
     2161 acggccgcct atcccgcatc cgccagaatc atccgcgccg ccttttcgcc gatcatgatc
     2221 gacggtgcgt tggtgttgcc ggaggtcagc gtcggcatga tcgaggcgtc cgccacccgc
     2281 agcccctgta tcccgtgcac ccgcagccgg tcgtccacca ccgccatcgg atcatgcccc
     2341 atcttgcagg tgccgaccgg atgataggtg gtctcggcat ggttcctgac ccagtccagc
     2401 atctcgtcat ccgactgcaa cgcggcgccg ggggcgatct cctcgcggac gatcccgtcc
     2461 atcggcggtg ccgccatgat gcggcgggtc aaccgcatga cttccagcgt cacgtcgcgg
     2521 tcgagctgcg cgctgaggaa gttgaagcgc accgcgggcg gggtctgcgc cgaagcggag
     2581 gtgatgtgga tgctgccggt gctctccgag cgcaggatgt tgacgatcgc ggtgatgccg
     2641 gagcgcttcg ccagtttgta gttggcatcg gccaggaacg gcagccaggc gatcgccgcg
     2701 ttgggcgcgt ccaggccggg acgggtgcgt acataggcgc ggatcggcga ggccggcagc
     2761 ccgagcaggc ccttgctggc ggtggcgtat cgcagcgcct gccagatcat acgcaggccg
     2821 cgccccttgt cgttgtaggt gaggcccagc gccggcggca ccagccattt catccgcggc
     2881 gagtagtggt cgcgcaggtt ctcgcccacg cccttcagtg cgtggcgcac gtcgatgccc
     2941 aggctttgca gcaactccgg ctggccgatg ccggacagtt ccagcaattg cggcgagttg
     3001 atcgaaccgg cgctgacgat cacctcccgc gccgcacgcg cctcgcgctg ctggccattc
     3061 accgtgtagc gcacgccggt gcagcgtttg ccgtccagca gcaggcattc ggtcagcgca
     3121 ttggcctgga tcgtcaggtt gggccggccg cgcgcgggat cgagatagca gacggcggtg
     3181 ctcatccggc gtccgttgcg gatggtcgcc tgggtcatgg cgatgccgtc ctgctgcgcg
     3241 ccgttgtagt cgcgcgtgaa gggaacgccg acggcgccgg cggcctggat cagcgcgtcg
     3301 tagatcggcc cgctctcatt gctttcggtg accttcagcg ggccgttgcg gccgcggtat
     3361 tcctcgtccg cggcgccctg gaagctctcc atcgccttga agatcggcag cacctcgcga
     3421 tagctccagc cgcgattccc aagctgcgcc caggtgtcat agtcctgcga ctggccgcgc
     3481 acgaacacca tgccgttgat cgacgacgag ccgccgagca gcttgccgcg cgggatcggg
     3541 atgcggcgct ggccggtgcc ggcatcgggt tcggaggaat acagccagtt ggcggcgggg
     3601 ttctcgatca gcttggcgaa gccgaccggg atgcgcgacc aggggtgact ctcgcgcccg
     3661 gcttccagca ccagcacctt gtgccgcggg tcggcgctga gccggttcgc cagcacggat
     3721 ccggcggatc cggcgccgac cacgatgtag tcgaatgtct ggctttccat tggcttcccc
     3781 cggcaggcgt tgcatgcatc gtcctgccgg cgggagccgg gcgcaatccc ccggttggac
     3841 ttcaccccat gtggtgagac acggaaatta cgcacagcct gcatccgccg tgggaccgca
     3901 ccgctgtgat gagacacaga aagagcccac gtgcgggagc tgcctccgta cgcgtgcggt
     3961 ggccgataag ccaaagctga gtgctgtcat tgccgattgc aggacttctt cgggaagtcc
     4021 cgctcgacgg attcgcgctc gccccggcac accgccaatg cgcatgagcc cgtgatcctg
     4081 cgag
//
LOCUS       DASSZH010000136         2501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_378440, whole genome shotgun sequence.
ACCESSION   DASSZH010000136 DASSZH010000000
VERSION     DASSZH010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378440"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..1130)
                     /gene="holA"
                     /locus_tag="VFN42_02875"
     CDS             complement(129..1130)
                     /gene="holA"
                     /locus_tag="VFN42_02875"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET8995588.1"
                     /translation="MKLAPQRTNAFLRDPGGCAVVLLFGEDVGLIQERAATLVRTVAG
                     SLDDPFLVVDLPRDRMTELADEAAALPLTGGRRVIRARDVSDSALNAVTALLKRPGPA
                     LVVLEAPGLASRSRLRTAVEAAPEGVAIGCYPDDPGQLETTIRDSLREDGIAIDPEAL
                     RWLSTQLGADRASTRSELLKLALYAGPGGRVDLDMAMHCVGDLAGLSLDDAVFAATEG
                     RVELADRALQLALGEGSSPVEVLRVGLNHLQRLHRARLLVDAGMPPAEATKSLRPPVF
                     FRKVPAFTKAVAQWSSAALAGAIAGFSEAERECKRTGAPDVVICRNAVLTLARRAAR"
     gene            complement(1137..1655)
                     /gene="lptE"
                     /locus_tag="VFN42_02880"
     CDS             complement(1137..1655)
                     /gene="lptE"
                     /locus_tag="VFN42_02880"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HET8995589.1"
                     /translation="MLQSVASLGGATLLAGCGFHPVYMPTASGGPGPAERDMAAIQVG
                     LIPDRPGQLLRQALQQRLGSDSGAEAPRYKLAVSYAIEGQGVAVAPDSTPTFTRLIGR
                     AHWSLTSVVPPHAPVTSGLARAYASMDVFQQQYFAADLETEQQQRVLARELAAQIAEQ
                     LAVFFRRRAGMS"
     gene            complement(1682..>2501)
                     /locus_tag="VFN42_02885"
     CDS             complement(1682..>2501)
                     /locus_tag="VFN42_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019088185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET8995590.1"
                     /translation="WLYPEEVDKQPDGTAVHRTSGTPVTVGRVEAMSKSKRNTVDPGA
                     IIARYGADTARWFILSDNPPERDMEWTEAGVAGANRFVQRVFRLAEGLDTEAGAKPDT
                     VGPAARALRQSTHRTIAAVTEALETFAFNVAVARLYEFANAIAAAERAGDEPGLGWAR
                     REALEILARLIAPMMPHLAEEVYARLLPNADRLVAELPWPEADPELLVADTVTIAVQV
                     MGKLRGTIAVPPDSPADSVLAAAEAEPNVARALEGRRIVKRVHVPNRIVNFVVA"
BASE COUNT          387 a          908 c          839 g          367 t
ORIGIN      
        1 gcatgggttt gttcctcacc aggttcacga tcggcctaac ggcgccaggt tgccgctgta
       61 ttacggcgcg gcgacatcta tgcgtcatac aatttattcc ggctagcccc cttttggctc
      121 gggccggcct agcgggccgc cctgcgggca agcgtcagga cggcgttgcg gcagatcacc
      181 acgtccgggg cacccgtgcg cttgcattcc cgttccgcct cactgaaccc ggcaatcgcg
      241 ccggccaatg ccgccgagga ccactgggcc accgccttgg tgaacgccgg gaccttgcgg
      301 aaaaacaccg gcggacgcag cgacttcgtc gcctcggctg ggggcatgcc ggcatccacc
      361 aacaggcgcg cccggtgcaa gcgctgcaag tggttgagcc cgacgcgcag cacctccaca
      421 ggggagctgc cttcgcccag ggcaagctgc agcgcccgat ccgccagctc cacccggccc
      481 tccgtcgcgg cgaacaccgc gtcgtccagg gaaagccccg ccaggtcgcc gacgcagtgc
      541 attgccatat ccaggtccac ccgaccgccc ggccccgcat atagggccag cttcagcagt
      601 tcggaccggg tggaagcccg atcggcgccg agctgggtgg acagccagcg aagcgcctcg
      661 ggatcgatgg caataccgtc ttcccgcaga ctgtcgcgga tggtcgtttc cagctggccc
      721 ggatcatccg gatagcagcc gatcgcgacc ccttccggcg cagcctcgac cgcggtgcgc
      781 agccgcgacc ggctcgccag cccaggcgcc tccagcacga ccaaggccgg cccgggccgc
      841 ttcagcagcg ccgtcacggc gttcagcgcg gaatcggaga catcccgagc acggatcacc
      901 cgccttccgc cggtcagcgg cagagccgcg gcttcatcgg ccagctcggt catgcggtcg
      961 cgcggcaggt caaccaccag gaatgggtca tccagcgaac ccgccacggt ccgcaccagg
     1021 gtggcggcgc gttcctggat cagccccaca tcctccccga acagcagcac caccgcgcag
     1081 ccaccggggt cgcgcaggaa agcgttggtc cgctggggtg caagcttcac gctggctcag
     1141 ctcatgccgg cgcggcggcg gaagaacact gcgagctgtt cggcaatctg cgccgccagt
     1201 tcccgcgcca gcacccgctg ctgctgctcg gtctccaggt cggcagcgaa gtactgctgt
     1261 tggaaaacat ccatcgaagc gtaggcgcgc gccaggccgc tggtgacagg cgcgtgcggc
     1321 ggcacgaccg aggtcaggct ccaatgcgct cggccgatca ggcgggtgaa ggtcggcgtc
     1381 gagtcgggcg cgacggcgac gccctggccc tcgatcgcgt agctgaccgc caacttgtag
     1441 cgcggcgcct ccgcgccgga gtcgctcccc aggcgctgct gcagcgcctg gcgcagaagc
     1501 tggcctggcc ggtcggggat caaacccacc tggatcgcgg ccatgtcgcg ttccgccggc
     1561 cccggtccgc ccgacgcggt cggcatatag accggatgga atccgcaacc ggccaacagc
     1621 gttgccccgc caagcgatgc gacggactgc agcacgcgac ggcgggtcca ggcaggcgcc
     1681 atcacgccac cacgaagttg acgatgcggt tcggcacgtg aacccgcttg acgatacgcc
     1741 ggccctccag cgcccgcgcc acgttgggct ccgcttcggc cgcggcgagg acgctgtcgg
     1801 ctggcgagtc cggcggcacg gcgatggtgc cgcgcagctt gcccatcacc tggacggcaa
     1861 tcgtgacggt atccgcgacc agcagttccg gatcggcctc cggccacgga agttcggcaa
     1921 cgagacggtc tgcgttgggg agcagccggg cgtacacctc ctcggcgaga tgcggcatca
     1981 tcggcgcgat cagccgcgcc aggatctcca gcgcctcgcg gcgtgcccaa cccagccccg
     2041 gttcgtctcc ggcgcgctcc gcggccgcga tggcattggc gaattcgtac aggcgggcga
     2101 cagcgacatt gaaggcgaag gtctccagcg cctcggtcac ggcggcaatg gtgcgatggg
     2161 tgctttggcg cagtgcacgc gcagcgggcc cgaccgtgtc cggcttggcg cctgcctcgg
     2221 tgtcgaggcc ttcggccagg cggaacaccc gctgcacgaa gcggttggcg ccggccacgc
     2281 ccgcctcggt ccactccatg tcacgctcgg gcgggttgtc ggacaggatg aaccagcggg
     2341 cggtgtccgc tccatagcgg gcgatgatcg cgccgggatc gaccgtgttg cgcttcgact
     2401 tgctcatcgc ctccacgcgg ccgacggtca ccggggtgcc gctggtccgg tgtactgcgg
     2461 tgccgtccgg ctgcttgtcc acttcctcgg gatacagcca c
//
LOCUS       DASSZH010000137         2830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1452491, whole genome shotgun sequence.
ACCESSION   DASSZH010000137 DASSZH010000000
VERSION     DASSZH010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1452491"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VFN42_02890"
     CDS             complement(<1..749)
                     /locus_tag="VFN42_02890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8995591.1"
                     /translation="MSRLRDRAVMLASAVGTMWGRGSQQLDAAESAPTARDTSNPGPD
                     NPALLTQFPSSVSPPATDKGHVPNLWFPFSQAHRRIQRGGWSREVTVQDFAAAREIAG
                     VNMRLTAGGVRELHWHLAAEWAYMLTGTAQITAVDQLGRRFVDNVGPGDLWYFPAGIP
                     HSIQGLEPDGCEFILAFDDGAFSEFDTFTISDWAGHTPKHVLAKNFGVDEAAFANVPR
                     EQLYIFQGRIVAAEPTEIGAPGVVPQSFSFRL"
     gene            complement(794..1012)
                     /locus_tag="VFN42_02895"
     CDS             complement(794..1012)
                     /locus_tag="VFN42_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995592.1"
                     /translation="MSKSPETKRYGGPGSSHQDREHPYRNNQPTGHKRGGDPSGALPG
                     EPTNGDESHVSGGGGERDRHHTHTAERS"
     gene            complement(1009..1197)
                     /locus_tag="VFN42_02900"
     CDS             complement(1009..1197)
                     /locus_tag="VFN42_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995593.1"
                     /translation="MTAHPPPVPPEQRPRHGVDPQAPQGEATAHPESDPRNLNTAEQS
                     RQGNTKQNTTNKGYQQDR"
     gene            complement(1298..2524)
                     /locus_tag="VFN42_02905"
     CDS             complement(1298..2524)
                     /locus_tag="VFN42_02905"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HET8995594.1"
                     /translation="MKAVVFHAIGDIRLEDVAEPKIQDPADAIVRLTTSAICGTDLHF
                     VRGTMAGMKPGTILGHEGVGIVEQFGAGVRNLDRGDRVVVCSTIACGSCSYCRAGYYS
                     QCDRANPGGKRAGTAFFGSPQAAGGFDGLQAEKARIPFANVGLVKLPDEVSDEQAILI
                     SDIFPTGWFGAELAEVTRGDTVAVFGCGPVGQFAIASARLKGAARIIAVDRYDDRLAM
                     ARRQGAEVVNFEKEDPVRTILQLTGEIGVDRVIDAVGVDAMHAHEGPAAEQAKQQEKQ
                     FRQQRQQVAPEQNPHDGHWVPGDAPSQALQWAVACVAKAGTIGVIGVYPPQAHTFPIG
                     ETMNKNLTVKAGNCPHRRYVPMLVDMVRAGVIDPVAILSKQEPMQQVIQAYEAFDKRE
                     PGWLKVAIEPLPAAAE"
     gene            2662..>2830
                     /locus_tag="VFN42_02910"
     CDS             2662..>2830
                     /locus_tag="VFN42_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014106680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET8995595.1"
                     /translation="MPDVPQPATETPAPQGRIVPVILSGGSGTRLWPVSRETFPKQLW
                     PLVSARTMMQET"
BASE COUNT          418 a          986 c          916 g          510 t
ORIGIN      
        1 aggcggaagc tgaaggattg cggcaccacg ccgggcgcgc cgatctctgt cggttccgcc
       61 gctacgatcc tgccctggaa aatgtacaac tgctcgcgcg gcacattggc gaacgcggct
      121 tcgtccacgc cgaagttctt cgccagcacg tgcttcggcg tgtggcccgc ccagtcgctg
      181 atcgtgaacg tgtcgaactc ggagaaggcc ccgtcgtcga acgccaggat gaattcgcag
      241 ccgtccggtt ccagtccctg gatggaatgc ggaattccgg cggggaaata ccaaagatcg
      301 cccggcccga cattgtccac gaagcgccgt ccgagctgat cgaccgcggt gatctgcgcg
      361 gtgccggtca gcatgtacgc ccattccgcc gccaggtgcc agtgcagctc gcgcacgccg
      421 ccggcggtca gccgcatgtt gacgccggcg atttctctgg cggcggcgaa atcctgcacc
      481 gtgacttcgc ggctccagcc gccacgctgg atgcggcgat gcgcctggga gaacggaaac
      541 cacagattgg gcacgtggcc cttgtccgtg gccggcggcg aaaccgaaga cgggaactgg
      601 gtcaacagcg ccggattgtc cggcccggga ttgctcgtat cgcgcgctgt gggggcggac
      661 tccgccgcat caagctgctg cgaaccccgc ccccacatcg ttccgaccgc ggaggccagc
      721 atcacggcac gatctcgaag ccgactcatc acgctgccct ccacgactgt gctgggaaag
      781 cccggcggat gcctcaggag cgctccgccg tatgcgtgtg gtggcggtcg cgctcaccgc
      841 cgccgccgga gacatggctt tcgtccccgt tggtcggctc gcccggcaat gcaccggacg
      901 gatcgccacc gcgcttgtgg ccggtcggct ggttgttcct gtatggatgc tcgcggtcct
      961 ggtggctgct gcctggtccg ccatagcgct tggtttctgg cgacttgctc atcggtcctg
     1021 ctgatagccc ttgttggtgg tgttctgctt ggtgttgccc tgacgcgatt gttccgcggt
     1081 gttcaggttg cgcggatcgc tttccggatg cgccgtggcc tcgccctgcg gcgcctgtgg
     1141 gtcgacgccg tggcgcgggc gctgttcggg cggaaccggc ggtggatgtg ccgtcatgcc
     1201 gaaactcctg ctgactgata catgaacggg gcgccggcga cccggttcgc cccgcggatg
     1261 ggctctccgc ctggatgcag gagcggagag cgcagcatca ctccgcggct gccggcagcg
     1321 gctcgatcgc caccttcagc cagccgggct cgcgcttgtc gaacgcttcg tacgcctgga
     1381 tgacctgctg catcggctcc tgcttcgaca ggattgccac cgggtcgatc accccggcgc
     1441 gcaccatgtc caccagcatc ggcacgtagc ggcggtgcgg gcaattgccg gccttcaccg
     1501 tcaggttctt gttcatcgtt tcgccgatcg ggaaagtgtg cgcctgcggc ggatacacgc
     1561 cgatcacgcc gatcgttccg gccttcgcca cgcaggccac cgcccattgc agcgcctggc
     1621 tcggcgcatc gcccggcacc cagtggccgt catgcggatt ctgctccggc gctacttgct
     1681 ggcgctgctg ccggaactgc ttctcctgct gctttgcctg ctccgccgcc gggccttcat
     1741 gcgcgtgcat cgcatcgacg ccgaccgcgt cgatcacccg atccacgccg atttcgccgg
     1801 tcagttgcag gatggtccgg accggatcct ccttctcgaa gttgaccacc tccgcgccct
     1861 ggcggcgcgc catcgccagc cggtcgtcgt aacgatccac cgcgatgatg cgggccgccc
     1921 ctttcagccg cgccgaggcg atggcgaact gtccgaccgg gccgcagccg aacaccgcga
     1981 ccgtatcccc gcgcgtcact tccgccagtt cggcaccgaa ccagccggtg gggaatatgt
     2041 cggagatcag gatcgcctgc tcgtcgctga cttcatcggg cagcttcacc aggcccacat
     2101 ttgcgaacgg aatgcgcgcc ttctccgcct gcaacccgtc gaagccgccg gccgcctgcg
     2161 ggctgccgaa aaacgcggtg ccggcccgct tgccacccgg attcgcccgg tcgcattgcg
     2221 agtagtagcc ggcacggcaa taggaacagc tcccgcaggc gatggtggaa cacaccacca
     2281 cgcggtctcc gcgatccagg ttgcgcacgc cggcaccgaa ttgctcgacg atgccgacgc
     2341 cttcatggcc caggatggtg cccggcttca tcccggccat ggtgccgcgg acgaagtgca
     2401 ggtcggtgcc gcagatggcg gaggtggtca ggcggacgat cgcgtcggcc ggatcctgga
     2461 ttttcggctc tgcaacatcc tccagccgga tgtcgccgat cgcatgaaat acaacggcct
     2521 tcataatctg ctccccgctt gccgtgagcg gcgggaacgc ctgcgccccg cgcggagttt
     2581 ggcgcagcgg cgaacgattg tctcgataag ccggtgacga tgcgctaagc gtaccgggat
     2641 ctttcaggaa ccgaggccca catgcctgac gtcccgcaac ccgccaccga gacgccggcg
     2701 ccgcagggcc ggattgttcc ggtcatcctg tccggcggct ccggcacccg gctttggccg
     2761 gtgtcgcgcg agacctttcc caagcagctt tggccgctgg tgtcggcgcg caccatgatg
     2821 caggagaccg
//
LOCUS       DASSZH010000138         2364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1435253, whole genome shotgun sequence.
ACCESSION   DASSZH010000138 DASSZH010000000
VERSION     DASSZH010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435253"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VFN42_02915"
     CDS             complement(<1..676)
                     /locus_tag="VFN42_02915"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8995596.1"
                     /translation="MDEQGAEPAAEIEEAPPPDWRRTMWAMVGIQFIMTLSFTILSPI
                     MPLFLPELGVTGAAHIDLWAGILNGTTSFVAAFASPLWGRIADQRGRKLMLLRSSFAI
                     GFFTVLMGLSLNVWQFFGARALMGAFAGFSASAIALVASQVPEERLGYALGWLSTGQL
                     VGGLVGPVLGGFLSDLSGSYRVPFFCTAAICFVAMGLAWAFVREHFTPPAQAGRGRSM
                     LSALRLL"
     gene            complement(722..1771)
                     /locus_tag="VFN42_02920"
     CDS             complement(722..1771)
                     /locus_tag="VFN42_02920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8995597.1"
                     /translation="MEFGLFHEFPSLPNHSDAEAFDAAFGLVDGAERWGLDAIWLAEL
                     HFDPARSVLSAPLCIASAIAARTQRMKIGIAVQVLPLCNPLRIAEEAATVDQISRGRL
                     IFGVGRSGVASTYEAYGVPYAESKERFAETLDIIQQAWTQPRVSYAGWFFRFQDVAVT
                     PRPYQQPLPEIRVAATSADTFATTGQQGLPIFLAVRHEDARMFAPQIAAYRAAWRAAG
                     HAGEGQVFLRAPGYLAATAEQARAEAEASLMHYYRAQAALLSDSATRAGVDAADHRAR
                     TAHRLASITYEEALQGSVFIGTPDSVTAQLRALQAELGLDGVLLEFNCGGRVPHAHEL
                     EALRLLCQAVMPRFH"
     gene            1880..>2364
                     /locus_tag="VFN42_02925"
     CDS             1880..>2364
                     /locus_tag="VFN42_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HET8995598.1"
                     /translation="MDALDFTEDEIRRYSRHILLREVGGTGQAKLKAARVLVIGAGGL
                     GSPLVLYLAAAGVGTIGIVDDDRVELSNLQRQIAHTTASIGSPKAASAADAARAINPG
                     VDIEIHPVRLNPDNALALIGSYDLVCDGTDNFPTRFLAADACVLARRTLVSAAVLRFD
                     G"
BASE COUNT          384 a          851 c          810 g          319 t
ORIGIN      
        1 tcagcagccg cagcgccgac agcatggatc ggccgcgccc ggcctgcgcc ggcggggtga
       61 aatgctcgcg cacgaaggcc caggcgaggc ccatcgcgac gaagcagatt gccgcggtgc
      121 agaagaatgg cacgcggtaa ctgccggaga ggtccgacag gaagccgccc agcaccgggc
      181 cgaccaggcc gccgacaagc tggccggtgc tgagccagcc cagcgcgtag cccaggcgct
      241 cctccggcac ctggctcgcc accagcgcga tcgcggaggc ggagaacccg gcgaaagcgc
      301 ccatcagcgc gcgggcaccg aagaactgcc agacgttgag cgacaacccc atcagcacgg
      361 tgaagaagcc gatggcgaag ctggagcgca gcagcatcag cttgcgcccg cgctggtcgg
      421 cgatacgccc ccacagcggc gaggcgaagg ctgccacgaa ggaggtcgtg ccgttcagga
      481 tgccggccca cagatcgata tgcgccgcgc cggtcacacc cagctcgggc aggaacagcg
      541 gcatgatcgg gctgaggatg gtgaaggaca gggtcatgat gaactggatg cccaccatgg
      601 cccacatggt gcgccgccag tccggcggcg gcgcttcctc gatctctgcc gccggttcgg
      661 cgccctgctc gtccatgccg gcgaaaatca cggcaggccg agcgattgta aagtccgcgg
      721 ctcagtggaa gcgcggcatc accgcctggc acagcagccg cagcgcttcc agctcgtgcg
      781 cgtgcggcac ccggccgccg cagttgaact ccagcagcac gccgtccagc cccagttccg
      841 cctgcagcgc ccgcagctgc gccgtcacgc tgtccggcgt gccaatgaac accgagcctt
      901 gcagcgcctc ctcataggtg atgctggcca gccggtgcgc ggtgcgcgcg cggtggtccg
      961 cggcatcgac cccggcgcgg gtggcggaat ccgacagcag cgcggcctgc gcgcggtagt
     1021 agtgcatcag gctggcctcc gcttccgccc gcgcctgctc cgccgtggcc gccaggtagc
     1081 cgggcgcgcg caggaacacc tgcccctcac cggcatgacc ggcggcgcgc caggcggcgc
     1141 ggtaggcggc gatctgcggc gcgaacatgc gcgcgtcctc atgccgcacc gcgaggaaga
     1201 tcggcagccc ctgctggccg gtggtggcga aggtatctgc gctggtggcg gcgacgcgga
     1261 tctccggcag cggctgctga tacgggcgcg gcgtcaccgc gacatcctgg aagcggaaga
     1321 accacccggc gtaggacacg cgcggctgcg tccaggcctg ctggataatg tccagcgtct
     1381 cggcgaagcg ctccttgctc tcggcatagg gcacgccgta cgcctcatag gtgctggcaa
     1441 cgccgctgcg gccgacgccg aagatcaggc ggccgcggct gatctggtcc accgtcgcgg
     1501 cttcctccgc gatgcgcagc gggttgcaca gcggcagcac ctgcaccgcg atgccgatct
     1561 tcatccgctg cgtgcgcgcg gcgatggcgc tggcgatgca cagcggtgcc gacagcacgg
     1621 agcgcgccgg atcgaaatgc agctccgcca gccagatcgc gtccagcccc cagcgctcgg
     1681 ccccatccac caggccgaac gccgcgtcga acgcctcggc atcgctgtgg ttcggcaagg
     1741 atgggaattc gtggaacagg ccgaactcca tggacgtctc tcccgttttg tttgcggcaa
     1801 cactatgccg ggtgccggac cggcggaagc cggccctgcc cttcctttcc caccgcgatc
     1861 cgcgcaccat atcgccgcca tggacgcact tgatttcacc gaagacgaga tccgccgcta
     1921 ttcccgccat atcctgctgc gtgaggtcgg cggcactggc caagccaagc tgaaggcggc
     1981 acgggtgctg gtgatcggcg ccggcggcct gggctcgccg ctggtgctgt acctggcagc
     2041 cgcgggcgtc ggcaccatcg gcatcgtcga tgacgaccgg gtggagctgt ccaacctgca
     2101 gcggcagatc gcccacacca ccgccagcat cggcagtccg aaggcggcgt cggccgcgga
     2161 tgcggcgcgc gccatcaacc ccggcgtgga catcgagatc caccccgtcc ggctgaaccc
     2221 ggacaacgcg ctggcgctga tcgggagcta cgacctggtg tgcgacggca cggacaattt
     2281 cccgacccgc ttcctcgccg ccgatgcctg cgtgctggcg cgccgcaccc tggtgtccgc
     2341 cgcggtgctg cgcttcgacg gcca
//
LOCUS       DASSZH010000139         3090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1435268, whole genome shotgun sequence.
ACCESSION   DASSZH010000139 DASSZH010000000
VERSION     DASSZH010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435268"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="VFN42_02930"
     CDS             <1..1057
                     /locus_tag="VFN42_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HET8995599.1"
                     /translation="GWIVPLPPARKTGKRVAVVGSGPAGMACAQQLARAGHDVTLFEK
                     SDRIGGLLRYGIPDFKMEKHHIDRRMAQMAAEGVTFRTGVEVGVTVDMDFLMGEYDAI
                     ALTGGAEWPRDLEVPGRELPGIHFAMDYLTQQNKRGAGDDEATAAPDGGISAAGKHVI
                     VIGGGDTGADCIGTAARQGAASILQLDILPKPPDKENKALTWPDWPLKLRTSPAHEEG
                     AERDWAVLTRRAVGVDRVQALECVRVEWAKGTDGRFVMRDVPGSTFELKADLVLLAMG
                     FLGPRRRLLEQAGLALDTRGNVLANTVDYRTSVSKLFAAGDMRRGQSLVVWAIREGRQ
                     CARAVDQFLMGTSLLPA"
     gene            complement(1152..2537)
                     /locus_tag="VFN42_02935"
     CDS             complement(1152..2537)
                     /locus_tag="VFN42_02935"
                     /inference="COORDINATES: protein motif:HMM:NF024935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET8995600.1"
                     /translation="MQTHASGDITTPLIPPVLLSWLAVFQPCFTAPTWQHVLVLVAGA
                     VLAPGKRTVTQVLRVMGLADHVGFARYHEVLNRARWDARALARQLLLHLLDKLLPSGP
                     VVIGIDDTIERRWGTKIKTRGIYRDPVRSSKGHFVKTSGLRWLSLMVVLPINWSGRSW
                     ALPFMTILAPSARWSETHRRRHKTLTDWARQAILQTKRWLPDRALIFVADSGFAALEL
                     LAAVRSHVCVITRLRLDAALYKPVPPRRKGQRGRSRLKGDRLPPLKSVLDSKKTVWTR
                     VVVSKWYNAEQRTLLTATGTALWYKAGIAPVPIRWVLVRDPTGEHEPAAFLSTDLRAT
                     PAMILGWFVSRWRVETTFQEVRTHLGVETQRQWSDLAILRTTPALLGLFSLITVWADD
                     LARNAGPALRPNIAAWYRKDQPTFSDAIAAVRRVLWCPPDFSMSRKPGETVQIPAELL
                     NRLVQTLCLAA"
     gene            2620..2901
                     /locus_tag="VFN42_02940"
     CDS             2620..2901
                     /locus_tag="VFN42_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995601.1"
                     /translation="MTDPVIEQIEAFRCRRGSLTAERRNRGYTLYNAHSGAPVARLRP
                     TGTEDRFHVLYWSLWKERWVASGPLGPAKLSIERALEFIAAEDIFWAIR"
BASE COUNT          541 a          993 c         1064 g          492 t
ORIGIN      
        1 aggctggatt gtcccgctgc cgccggcgcg caagaccggc aagcgcgttg ccgtggtggg
       61 ctccggcccc gccggcatgg cctgcgcgca gcaactggcc cgcgccgggc atgacgtgac
      121 gctgttcgag aagtcggacc gtatcggcgg cctgctgcgc tacggcattc ccgacttcaa
      181 gatggagaag caccacatcg atcgccgcat ggcgcagatg gcagcggaag gcgttacttt
      241 ccgcaccggc gtcgaagtgg gcgtgaccgt cgatatggac ttcctgatgg gcgagtatga
      301 tgccatcgcg ctgaccggcg gcgccgaatg gccgcgcgac ctggaagtgc ccgggcgcga
      361 gctgcccggc atccatttcg ccatggacta cctgacgcag cagaacaaac gcggcgccgg
      421 cgacgacgag gccaccgcgg cgccggatgg cggcatcagc gcggcaggca agcatgtcat
      481 cgtgatcggc ggcggcgata ccggcgccga ctgcatcggc accgcggcgc gccagggtgc
      541 ggcctccatc ctgcaactgg acatcctgcc gaagccgccg gacaaggaga acaaggcgct
      601 gacctggccg gactggccgc tgaagctgcg cacctccccg gcgcacgagg aaggcgcgga
      661 gcgcgactgg gcagtgctga cccggcgcgc cgtcggcgtg gatcgagtgc aggcgttgga
      721 atgcgtgcgg gtggagtggg cgaaggggac ggacggccgc ttcgtcatgc gcgacgtgcc
      781 cggcagcacg ttcgaactga aggcggacct ggtgctgctg gcgatgggct tcctcggtcc
      841 gcggcgccgg ctgctggagc aggccggact ggcgctggac acgcggggga acgtgctggc
      901 gaacacggtg gactaccgga cgtcagtgtc gaagctgttc gccgccggcg acatgcggcg
      961 cggccagtcg ctggtggtgt gggcgatccg tgaggggcgg cagtgcgccc gggcggtgga
     1021 ccagttcctg atggggacca gcctgctgcc ggcctgaacg ccccaccgcg gcccgccccc
     1081 gcgcctggcg cggccccagc cgaaccagca gcgcagggcg ctgtccggca ctcagctcga
     1141 ctttgcgcga ttcaggcggc gaggcagaga gtctggacga gtcggtttag caattcggcg
     1201 ggaatttgca cggtttcccc gggcttccgc gacatggaaa aatccggcgg gcaccaaagc
     1261 acgcggcgta ctgcggcgat cgcatcgctg aaggttggtt ggtccttgcg gtaccaggcc
     1321 gcgatgttcg gccgcagcgc agggccggcg ttgcgagcca gatcatcggc ccacacggtg
     1381 atcagggaga acaggccaag cagtgccggg gtggtgcgca ggatggcaag atcggaccac
     1441 tgacgctggg tttcgacgcc gagatgtgtt cgcacttctt ggaatgtggt ctcgacacgc
     1501 cagcgcgaga cgaaccagcc gagtatcatc gccggcgttg ccctcaggtc ggtgctgagg
     1561 aaggctgccg gctcgtgctc cccggtcgga tcgcgcacca gcacccagcg gattggcact
     1621 ggcgcgatgc cggccttgta ccagagcgcg gtgccggttg cggtcagcag agtacgctgt
     1681 tcggcattgt accacttgga gacaacaacg cgggtccaca cggtcttctt gctgtccagc
     1741 acgctcttga gcggcggcag acgatcgccc ttcagccggg agcgaccgcg ctggcccttc
     1801 cggcgcggcg ggaccggctt gtacaaggcg gcatccagcc gcagccgcgt gatcacacag
     1861 acgtggctgc gcaccgcggc aagcagttcg agtgcggcaa acccactatc ggcgacaaag
     1921 atcagcgcac ggtcgggcag ccagcgcttg gtctggagaa tggcttgccg tgcccagtcg
     1981 gtcagcgtct tgtgccgcct gcgatgcgtc tcgctccagc gggccgacgg cgccaggatg
     2041 gtcatgaagg gcagagccca gctccggccg gaccaattga tcggcaacac gaccatcagc
     2101 gacagccagc gcaggccgct ggtcttgacg aagtgcccct ttgaggaccg cacggggtcg
     2161 cggtagatgc cgcgggtctt gatcttcgta ccccaccgcc gctcgatggt atcgtcgatg
     2221 ccaatgacca ccggtccgct gggcagcaac ttatccagca gatgcagcaa caactgccgg
     2281 gccagggcgc gcgcatccca ccgtgcccgg ttcagcacct catgatagcg ggcgaagccg
     2341 acatgatcgg ccaggcccat cacccggagc acctgggtga cggtgcgttt gccgggcgcc
     2401 agcaccgcgc cggcgaccag caccaggacg tgctgccagg tgggggcggt gaaacagggc
     2461 tggaacacgg cgagccagct gagcagcacc ggcgggatca gcggggtggt gatgtcaccg
     2521 gaagcatgtg tttgcatccg agtcgtcgag tcgagacgca ggtgattcgc aagagtccgg
     2581 ccacgacagc acgccaattt gccggctaat ctgccggcca tgaccgatcc cgttatcgaa
     2641 cagatcgagg cgttccgttg ccgccggggc agcctgaccg ccgagcggcg caaccgcggc
     2701 tacacattat acaatgccca ttccggcgca ccggtcgccc gcctgcggcc gaccggcacc
     2761 gaagaccgct tccacgtgct ctactggtct ctctggaagg aacgatgggt tgcatccggc
     2821 ccgctcggcc cggccaagct ctcgatcgag cgggcactcg agttcatcgc cgcagaggat
     2881 atcttctggg cgatccgctg accccacgcc caatccaaaa atgcgcaaag tcgagcttag
     2941 cgcagccaaa aaacaggaga atttcgcccg cgagtttgta aaatcccgag taatggaatt
     3001 gattgctatg cctccctact ccgatcttgc ttacgtgcgg tcgtggctgt tgaatttgtt
     3061 tattgatggt actctgccgg ttaccctctc
//
LOCUS       DASSZH010000140         5049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_719324, whole genome shotgun sequence.
ACCESSION   DASSZH010000140 DASSZH010000000
VERSION     DASSZH010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719324"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..881)
                     /locus_tag="VFN42_02945"
     CDS             complement(216..881)
                     /locus_tag="VFN42_02945"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET8995602.1"
                     /translation="MAAIRNVLWIMCDQLRFDYLSCYGHPHLQTPHIDSLAARGVRFT
                     RAYVQSPVCGPSRMSFYTGRYMRSHGANWNRFPLRVDEPTLGDHLGELGVRNVLVGKT
                     HMEADLAGMRRLGIDPASSLGVHVSQCGFEPFERDDGLNPDPAPRVPYDDYLRERGFG
                     GDNPWQDWAASAEDADGSLLNGWLLAHAGKPARVPEEHSETPYMTRRAMDFIDGAATD
                     GRP"
     gene            complement(1100..1996)
                     /locus_tag="VFN42_02950"
     CDS             complement(1100..1996)
                     /locus_tag="VFN42_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HET8995603.1"
                     /translation="MRRIMLALAVLLLAAPSLARAQGEPQIVVDRATLTVQEMMSNRQ
                     SEDPRGLLRRARAVLICPRVFKAGFFFGGSGGDCVLLGRAGNGTWSYPAFYAIGSGSF
                     GFQFGIEDSSIVMMIMTNRGLNAVLDSHFKIGAGAGLAIATIGAGVQGSTTAAVGADI
                     VAFAQSRGLYGGISLEGSILSSRSGWDQAYYRQPLGSRQIVMQMQGANPGADPLRAVL
                     TRYGSVPPMNRPPAYSYQQQRPAYSYGQPPSGYAQQGQGYGAGQAPTGPGYAPAYPPM
                     QQATPGNYAPVQQQNLPPPPNR"
     gene            complement(2081..2701)
                     /locus_tag="VFN42_02955"
     CDS             complement(2081..2701)
                     /locus_tag="VFN42_02955"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET8995604.1"
                     /translation="MSETRFWLVRHALVEENARAILYGVMDVEVCEATLLEQGPMYRS
                     LAQRLPRPAVWKVSPLARTRRTAEAIFAAGYPSVPLSEEPDLIEQALGEWQGLPHAEL
                     PARLTLPKHAFWPLGGEERPPGGESMAEVIARVGPALERLAREHAGSDVVAVSHGGTI
                     RAAVAHALRIGPDNALHLSIQNLSLTRLERHPEGWRVVCVNELPGY"
     gene            2777..4102
                     /locus_tag="VFN42_02960"
     CDS             2777..4102
                     /locus_tag="VFN42_02960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8995605.1"
                     /translation="MAPRRLHLGAFMRPISIHTAAWRYPGAYPDANFNLSHLKRFART
                     LERGKFDAFFMADHLAVLNMPMDALKRSATVTSFEPLTLLSALAMCTERLGLIATGST
                     TFDLPYHVARRFASLDHLSEGRAGWNIVTTSNPDAALNFGLDEHLEHDERYRRAREFY
                     DVVTGLWDSWAEDAFIRDQQSGVYFDPARMHVLNHKGPYYSVRGPLNIGRPVQGWPVI
                     VQAGASDAGRQFAAEVSEVIFAGAGRIEDARAFYADIKARTEAAGRDPDHLKILPGAF
                     VVVGDSLEEAQEKRARLDSLVHYDSAIASLSIALGHDASKFDPDAPLPDIPESNASQS
                     GRARTIALAKRENLTVRQLAQRLGGYAGPGFVGTPQMIADQMEEWLVTRASDGFNVMF
                     PYLPGGLDDFVDRVIPELQRRGLFRREYEGTTLRDHLGLPRPENRFFAR"
     gene            complement(4344..>5049)
                     /locus_tag="VFN42_02965"
     CDS             complement(4344..>5049)
                     /locus_tag="VFN42_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET8995606.1"
                     /translation="LPHLPQAFDGYTLLHLTDLHLDAAPDVTGAVINAVHGLQYDACV
                     LTGDYRARTFGAFDAALAGMAELRPHLQGPVYATLGNHDTLRMVPALEAMGYTLLINE
                     WTTLQRDGESIYLAGIEDAHFYRLENFHRTADAIPAHAVSILLSHTPEPYRRAAHADF
                     DLMLSGHTHGGQICLPGGRPILTDADSPRRISRGAWRYHQMVGYTSTGAGTSIVDARL
                     NCPPEITLHRLRRGGV"
BASE COUNT          790 a         1772 c         1711 g          776 t
ORIGIN      
        1 cccatatagg ccgggatcac aagctgccgc acgtcgtcgc gggcgaagtt gcgcgacacc
       61 cgcgcctgca tgaaggcgcc atacaccgga tgcgggtcct gccgttcacg ctcatgccgc
      121 accggcggga cgatgtgctg cggcccgtac agcgcgtggt acggcgccgg cgcgatgtac
      181 ggccagtgcg gcttgatgta ggacagatgc aggcactacg ggcgcccgtc cgttgccgca
      241 ccgtcgatga agtccatggc gcggcgggtc atgtaggggg tctcgctgtg ctcctccggc
      301 acgcgcgccg gcttgccggc atgcgccagc agccagccgt tcagcagcga cccgtccgcg
      361 tcttccgccg aggcggccca gtcctgccag ggattgtcgc cgccgaatcc ccgctcgcgc
      421 aggtaatcgt catagggcac ccgcggcgcc ggatcggggt tcagcccgtc atcgcgctcg
      481 aacggctcga agccgcattg cgacacgtgc acgccgagcg aggaggccgg gtcgatgccg
      541 agccggcgca tgccggccag gtcggcctcc atatgcgtct tgcccaccag cacgttgcgc
      601 acgcccagtt cccccaggtg gtcgcccagg gtgggctcgt ccacccgcag cgggaagcgg
      661 ttccaattgg cgccgtggct gcgcatgtag cggccggtgt agaagctcat gcgcgagggg
      721 ccgcagaccg gcgactgcac ataggcgcgg gtgaagcgca cgccgcgcgc ggccagcgag
      781 tcgatatgcg gcgtctgcag atgcggatgg ccgtagcagc tcaggtaatc aaaccggagc
      841 tggtcgcaca tgatccacag cacgttgcgg atagccgcca tggatggagt ccccccgagg
      901 ttggcggagg atggtaaggc ggcaggcgcg agaaggaagg ggggcggccg gaaacgcccc
      961 ctccccttgc gggcttgggc cgcgaagcgg accaaggatg gggggagggg gctgttgtgc
     1021 agtccccggg gaactcccga ccgccccctc cccctacccc ctcccgcaag gggaggggga
     1081 gattctggac gctccgcccc tagcggttcg gtggcggcgg caggttctgt tgctgcaccg
     1141 gggcatagtt gcccggcgtg gcctgctgca tcggcggata ggccggcgcg taacccgggc
     1201 cggtcggcgc ctgccccgcg ccgtagccct ggccctgctg tgcatagccg gatggcggct
     1261 ggccgtagga ataggcggga cgctgctgct ggtaggaata ggccggtggc cggttcatcg
     1321 ggggaacgct gccgtagcgc gtcagcacgg cccgcagcgg gtcggcgccc gggttggcgc
     1381 cctgcatctg catcacgatc tgccgcgacc ccaacggctg gcggtaatac gcctggtccc
     1441 agccgctgcg cgacgacagg atgctgcctt ccagagaaat cccgccatac agcccgcggc
     1501 tctgcgcgaa ggccacgatg tcggcgccca ccgccgcggt ggtgctgccc tgcacccccg
     1561 cgccgattgt cgcgatggcc agcccggccc cggcgccgat cttgaaatgg ctgtccagca
     1621 ccgcgttcag cccccggttg gtcataatca tcatgacgat ggagctgtcc tcgatgccga
     1681 actggaagcc gaagctgccg ctgccaatgg cgtagaaggc cgggtacgac catgtgccgt
     1741 taccggcgcg tcccagcagc acgcaatcgc cgccggagcc gccgaagaag aacccggcct
     1801 tgaacacccg cggacagatc agcacggcgc gggcgcggcg cagcaatccc cggggatcct
     1861 ccgactgcct gttgctcatc atctcctgca cggtcagcgt ggcccggtcc acgacgatct
     1921 gcggctcgcc ctgtgcccgg gcgaggctcg gggcggcaag cagcaggacg gcgagggcca
     1981 gcatgattcg gcgcatcggt cgacctcctc caactccaga atcgcgcctg catagcgcgg
     2041 ctggcctgga ttcgtacgct ggattcgggc ggatttccgg tcagtaacca ggaagttcat
     2101 tcacgcagac cacccgccac ccctccgggt ggcgctccag ccgggtcagc gacaggttct
     2161 ggatggacag atgcagcgca ttgtccgggc cgatgcgcag cgcgtgtgcc accgcggcgc
     2221 ggatcgtgcc gccgtgactc accgccacca cgtcgctgcc ggcatgttcc cgcgccagcc
     2281 gctccagcgc cgggccgacc cgggcgatca cctccgccat gctctccccg cccggcgggc
     2341 gttcctcgcc gcccagcggc cagaacgcgt gcttcggcaa ggtcagccga gcggggagtt
     2401 cggcgtgggg caggccctgc cattcgccca gggcctgctc gatgaggtcg ggttcctcgc
     2461 tcagcggcac gctcgggtag ccggcggcga agatcgcctc ggcggtgcgg cgggtgcggg
     2521 ccagcggcga caccttccac accgccggcc gcggcaggcg ctgcgccagg gagcggtaca
     2581 tcggcccctg ctccagcagc gtggcctcgc acacctccac gtccatcacg ccatacagga
     2641 tggcgcgggc gttctcctcc accagcgcgt ggcggaccag ccagaagcgc gtctcgctca
     2701 tgcccgcccg cctctcgaca atacccggcg cagccccatc ctaggtccaa cctttcctgt
     2761 ctcgggacac cggatcatgg ccccccgccg cctgcatttg ggcgccttca tgcgcccgat
     2821 cagcatccac accgccgcct ggcgctatcc cggcgcctat ccggatgcca acttcaacct
     2881 cagccacctg aagcggttcg cacgcacgct ggaacgcggc aagttcgacg cgttcttcat
     2941 ggccgatcac ctcgccgtgc tgaacatgcc gatggacgcg ctgaagcgca gcgccaccgt
     3001 cacgtcgttc gagccgctca ccctgctttc cgccctcgcc atgtgcaccg agcggctggg
     3061 gctgatcgcc accggctcca ccaccttcga cctgccctac cacgtcgcgc gccgcttcgc
     3121 ctcgctggac catctcagcg agggccgcgc cggctggaac atcgtcacca cgtccaatcc
     3181 ggatgccgcg ctgaatttcg gcctggacga acacctggaa cacgatgagc gctacaggcg
     3241 cgcgcgcgag ttctacgatg tggtcaccgg gctgtgggat tcctgggcgg aggacgcgtt
     3301 catccgcgac cagcagagcg gggtgtattt cgatccggcg cgcatgcacg tgctgaacca
     3361 caaggggccg tattactcgg tgcgcggccc gctgaacatc ggccgtccgg tgcagggctg
     3421 gccggttatc gtgcaggccg gcgcatcgga tgccgggcgg cagttcgccg ccgaggtgtc
     3481 ggaggtgatc ttcgccggcg ccggccgcat cgaagatgcc cgcgcgttct acgccgacat
     3541 caaggcgcgg acggaagcgg ccgggcgcga tccggatcac ctgaagatcc tgccgggcgc
     3601 cttcgtcgtt gtcggtgact cgctggagga ggcgcaggag aagcgcgcgc ggctggacag
     3661 cctggtgcat tacgacagcg ccatcgcgtc gctgtcgatt gcgctggggc atgacgcgtc
     3721 gaaattcgat ccggacgcgc cgctgccgga catcccggaa agcaacgcca gccagagcgg
     3781 ccgcgcgcgg acaattgcgt tggcgaaacg cgagaacctg acggtgcgcc agttggcgca
     3841 gcgcctgggc ggctatgccg gaccgggctt cgtcggcacg ccgcagatga tcgccgacca
     3901 gatggaggaa tggctggtca cccgcgccag cgacggcttc aacgtgatgt tcccgtatct
     3961 gcccggcggg ctggacgact tcgtcgaccg ggtgatcccg gaactgcagc gccgcggcct
     4021 gttccggcgc gagtacgagg ggaccacgct gcgggatcat cttggcctgc cgcgtccgga
     4081 gaaccggttc ttcgcgcgtt agtgccctga caccaagcgc caatgccatg gcggccgcca
     4141 gctatgatgc cgccccttcc ccttctgtca tgcccgtgct cggcacgggc acccacgtct
     4201 tgattggacg cagcagcaag aagccgtggg tggccgcgcc aagcgcggcc atgacacata
     4261 ggtcgatccg gccatgacac attggtcgat ccggccatgc ccctaggtcg atccggccat
     4321 gccactgcga tgatgtcggt gcatcacacc ccgccgcggc gcaggcggtg cagcgtgatc
     4381 tccggcgggc agttcagccg ggcatcgacg attgacgtgc ccgctccggt ggaggtgtag
     4441 ccgaccatct gatgatatcg ccacgcgccg cgggagatcc gccgcggact gtcggcgtcg
     4501 gtcaggatcg gccggccgcc cggcaggcag atctgcccgc catgcgtatg accgctcagc
     4561 atcaggtcga aatcggcatg cgcggcgcgg cggtacggct ccggcgtgtg cgacagcagg
     4621 atcgacaccg cgtgcgccgg aatcgcgtcc gccgtacggt ggaaattctc caaccggtag
     4681 aaatgcgcgt cctcgatgcc ggccaggtag atcgactcgc catcgcgctg cagcgtggtc
     4741 cactcattga tcagcagcgt gtagcccatc gcctccagcg ccggcaccat gcgcagcgtg
     4801 tcgtgattgc ccagcgtcgc gtacaccggt ccctggagat gcggccgcag ctcggccatg
     4861 ccggccaggg ccgcgtcgaa ggcaccgaag gtgcgcgcgc gataatcacc agtcagcacg
     4921 cacgcatcgt actgcaaccc gtgcaccgca ttgatcaccg cgccggtcac gtccggtgcg
     4981 gcgtccaggt gcaggtcggt caaatgcagc agcgtgtagc cgtcgaaggc ctgcggcaga
     5041 tgcggcagc
//
LOCUS       DASSZH010000141         2284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_139760, whole genome shotgun sequence.
ACCESSION   DASSZH010000141 DASSZH010000000
VERSION     DASSZH010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139760"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VFN42_02970"
     CDS             <1..224
                     /locus_tag="VFN42_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HET8995607.1"
                     /translation="SPMVGVAYLSPEPGSPPFVTVGQTVTAGQTLLLIEAMKTFNQIK
                     APKAGTVARVLVTSGTPVEYGEVLMLLDP"
     gene            243..1583
                     /gene="accC"
                     /locus_tag="VFN42_02975"
     CDS             243..1583
                     /gene="accC"
                     /locus_tag="VFN42_02975"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET8995608.1"
                     /translation="MFQKILIANRGEIALRIQRACRELGIPTVAVHSTADAEAMHVRL
                     ADESVCIGPPPAKDSYLNIPAILSAASITGADAIHPGYGFLSENARFAEMVEAHGMTF
                     IGPSPEHISMMGDKIAAKEAMGGLGVPLVPGSDGAVLDLESARTVAAKVGYPVLIKAA
                     AGGGGRGMKVAQDEAGLEEAFRVARSEAAAAFGNDADYLEKYLDRPRHIELQVFADAH
                     GNVVHFGERDCSLQRRHQKLLEEAGSPALSAAARDELGRTATAALRKLGYRNAGTLEF
                     LYQDGQFAFIEMNTRLQVEHPVTEMVCGIDLVREQIRIAAGAALGYGQEEIGFTGHAI
                     ECRITAEDPDSFVPTPGRVSVFHAPGGLGVRVDSALYAGYFVPPYYDSMVAKLIVHAP
                     TRIDAINRMRRALDEFVVVGIQTTIPLHQRIVDDPQFQSGDYTIHWLERFVARG"
     gene            complement(2011..>2284)
                     /locus_tag="VFN42_02980"
     CDS             complement(2011..>2284)
                     /locus_tag="VFN42_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995609.1"
                     /translation="ILALARRGKIGVVIAAICRDLGLVPSVVSEQQWKDVADLITAFG
                     GNFVALIGDAMNRVLSYIHAASFTKPLSGAEIGILNDAIFLRPAPA"
BASE COUNT          379 a          720 c          809 g          376 t
ORIGIN      
        1 ccagcccgat ggtcggcgtg gcctatctgt cgccggaacc cggaagcccg cctttcgtca
       61 ccgtcggcca gacggttacc gccgggcaga cgctgctgct gatcgaagcg atgaagacgt
      121 tcaaccagat caaggcaccc aaagccggaa ccgtcgcccg cgtgctggtc accagcggca
      181 cgccggtcga gtacggtgag gtgctgatgc tcctggaccc ctgaacttcc ggacggattc
      241 tcttgttcca gaagattctc atcgccaatc gcggcgagat cgcgcttcgc atccagcgcg
      301 cctgccgcga actgggcatt cccaccgtcg cggtgcactc caccgccgac gccgaggcga
      361 tgcatgtccg cctcgcggac gagagcgtgt gcatcggccc gccgccggcc aaggacagct
      421 atctgaacat tccggcgatc ctgtccgccg ccagcatcac cggcgccgat gcgatccatc
      481 ccggctacgg cttcctgtcg gagaacgccc gcttcgccga gatggtggaa gcgcacggca
      541 tgaccttcat cggcccgtcg cccgagcaca tcagcatgat gggcgacaag atcgcggcga
      601 aggaggcgat gggtggactg ggcgtgccgc tggtgcctgg gtcggacggc gcggtgctgg
      661 acctcgaatc ggcgcgcacg gtggcggcga aggtcggcta tccggtgctg atcaaggcgg
      721 cggccggcgg cggcggccgc ggcatgaagg tggcgcagga cgaggccggc ctggaggagg
      781 cgttccgggt cgcgcgcagc gaggcggcgg cggcgttcgg caacgacgcg gactatctgg
      841 agaaatacct cgaccgcccg cggcatatcg agctgcaggt gttcgccgat gcgcacggca
      901 acgtggtgca tttcggcgaa cgcgattgca gcctgcagcg gcggcaccag aagctgctgg
      961 aggaggctgg ctcccccgcg ctgagcgcgg cggcgcgcga cgagctgggc cgcaccgcga
     1021 cggcggcgct gcgcaagctg ggctaccgca atgccggcac gctggagttc ctgtatcagg
     1081 acggccagtt cgcgttcatc gagatgaaca caaggctgca ggtggagcac ccggtcaccg
     1141 agatggtgtg cggcatcgac ctggtgcgcg agcagatccg catcgcggcg ggggcggcgc
     1201 tgggctacgg ccaggaggag atcggcttca ccggccacgc catcgagtgc cgcatcacgg
     1261 cggaggaccc ggacagtttc gtgccgaccc cgggccgggt gagcgtgttc cacgcgccgg
     1321 gcgggctggg cgtgcgggtg gattccgcgc tgtacgccgg gtatttcgtg ccgccttatt
     1381 acgacagcat ggtagcgaag ctgatcgtgc acgcgccgac ccggatcgac gcgatcaacc
     1441 ggatgcggcg cgcattggac gagttcgtgg tggtggggat tcagaccacg atcccgctgc
     1501 atcagcgcat tgtcgatgat ccgcagttcc agtcaggcga ttacacgatc cactggctgg
     1561 agcgcttcgt cgcgcggggt taggccgcgc gctgacgccg attctccggt gtcatggtcg
     1621 gcgcaggcgc ccggcgaccc acgtcttctc tttggcgcac acgcaaaaga cgtaggtgcc
     1681 cgcgccaaac gcgggcatga cacagggggg gcaacggttg cgcggattgg catcacgcgg
     1741 ggccggcggc gttggtgcag ccaggattcg agaatccgct ttgccgacct tgagatatca
     1801 ataaacttgt ttggtgcgcc cgtattgcgg ggtgacggtt ctgcgaactc tgcggttctc
     1861 tgcgtctctg cgcggaatgc ggtgctgcca gcgtcgagac ctgacatggt gggtaccggg
     1921 cgtcaggttg cagtccttcg catgagggaa tcgccaagcg cgggaccacc catgcgaggg
     1981 tcccgtgcat accgcatcac gccgcccggt tcaggctggc gcgggtcgca ggaagatcgc
     2041 gtcgttcaga ataccgatct ccgcgccgct cagcggcttg gtgaacgatg ccgcatggat
     2101 gtagctgagc acacggttca tcgcgtcgcc gatcagtgcg acgaaattgc cgccgaaggc
     2161 ggtgatgagg tcggctacgt ccttccattg ctgttcggag acgacgctgg gcaccaggcc
     2221 gaggtcacgg cagatcgccg cgatgaccac gccgattttg ccgcggcggg ccaaggcgag
     2281 gatc
//
LOCUS       DASSZH010000142         2970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_957874, whole genome shotgun sequence.
ACCESSION   DASSZH010000142 DASSZH010000000
VERSION     DASSZH010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957874"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VFN42_02985"
     CDS             complement(<1..296)
                     /locus_tag="VFN42_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET8995610.1"
                     /translation="MFSLPELEAAHRLVHAVFPGTPQYAWPLLAQRCGAEVWVKHENH
                     TPTGAFKLRGGLVYAERLARERPDVTGIVSATRGNHGQSLAFAGQRYNLPVTIVV"
     gene            complement(387..590)
                     /locus_tag="VFN42_02990"
     CDS             complement(387..590)
                     /locus_tag="VFN42_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET8995611.1"
                     /translation="MAIGTVKWFNATKGYGFIMPQDGGKDVFVHITAVQAAGLKGLND
                     GQKVTYEVTKERGKDAAINLRLA"
     gene            complement(694..897)
                     /locus_tag="VFN42_02995"
     CDS             complement(694..897)
                     /locus_tag="VFN42_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET8995612.1"
                     /translation="MATGTVKWFNTTKGYGFIMPQDGGKDVFVHITAVQAAGLRGLNE
                     GQKVTYEVQMERGKAAATNLRLA"
     gene            1303..2868
                     /locus_tag="VFN42_03000"
     CDS             1303..2868
                     /locus_tag="VFN42_03000"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HET8995613.1"
                     /translation="MALASAIVTATDFARHQARLRPAAPAFWFESQETSFRELDMRSS
                     QCAQALLAAGVRPGERIAVLSKNNDAFPVLWFGAMKARACTVPVNIRLAPPEIAAILR
                     DSDATHLLVGHDFAALAEQIAEQCPMLKTVCQFEPGHPRLAGFDSWIAPFPPEDPCLP
                     EQPDDDIIQLYTSGTTGLPKGVPLTHRNCMAQCESGTQQSYAQWQPEKSSLLALPVFH
                     IAGALVALLSVLHGTRAVMVREIVPAELARVIAEQRVAFAFLTPTVIQMILSAPESAN
                     ADYSALEHVFYGASPISEALLRRAMARLPCQFTQVYGMTEATGVVTALPPEKHVPGKL
                     LSCGAPIAGVELRVIDAEGRDVPQGQVGEVALRAPFIMRGYWKQPEATAAAIDANGWY
                     RSGDAGWVDADGDLFIHDRVKDMIVSGGENVYPAEVENAIDGHPDVAEVAVIGVPDER
                     WGEAVKAIIVPKPGAPRDAGSIIAWARRRIGGFKVPKSVDFAEALPRNATGKVLRRML
                     RDQYWTGSTRRVG"
BASE COUNT          491 a          961 c         1024 g          494 t
ORIGIN      
        1 accacgatgg tcaccggcag gttgtagcgc tgcccggcaa aggccagcga ctggccgtgg
       61 ttgccgcggg ttgccgagac gatgccggtg acgtccggcc gctcgcgcgc caggcgttcg
      121 gcgtagacca gcccgccgcg cagcttgaag gcgccggtgg gggtgtggtt ttcgtgcttg
      181 acccagacct cggcgccgca gcgttgcgcc agcagaggcc aggcatactg cggcgtcccc
      241 ggaaacacgg cgtgcaccag gcggtgcgcc gcctccagtt caggcagcga gaacatcggt
      301 ggcactccca aacgcaacag gccgccgtcc agggcgaaca ccccggccgg cggcccggtc
      361 tcggtatcag gcgatgacgc tggacgtcag gcgagtcgga gattgatcgc ggcgtccttg
      421 ccgcgctcct tggtcacctc ataggtcacc ttctggccgt cattcaggcc tttcaggccc
      481 gcggcctgga cggcagtgat gtggacgaac acgtccttgc cgccgtcctg cggcattatg
      541 aagccatagc ccttcgtggc attgaaccac ttcacggttc caatcgccat ggtctggtca
      601 ctccttccga tgcgcgtccc ccccaggtcc cagcctggcc gcgcgagtgg ggatgggtga
      661 tggcaacgtg gtttggaccc gcgtgctgcg gcctcaggcg agccggaggt tggtggcggc
      721 agccttgccg cgctccatct gcacctcgta ggtcaccttc tggccttcat tcagaccgcg
      781 caggccggcc gcctgcaccg cggtgatatg gacgaacacg tccttgccgc cgtcttgcgg
      841 cataatgaag ccatagccct tcgtggtgtt aaaccacttg actgtaccag tagccataac
      901 tgcgtcgttc tccgtcaggt gcgccgcaac cgcgcggcgc gatagacaac cggccctttg
      961 gacgtcttga gggcacccga tgacggaggc gcagcaagcc tagccaaaat gcgattgcgt
     1021 tgcacagttg gcacagaaac gtggcctcgt gtcaatcacg gttgcgctac cgagacgcac
     1081 gacggtgcga gcctaggctg agggcaaagc cacccaagca caaaaccagc ggcatgtcgc
     1141 atttatatta tgacacgtac ccgcaagcga gattccgccc agccgctttg agccgggggc
     1201 gggcggccgg cacgcgaggc cacagtgcag gtgacgccgc cgcggctccc ggcgcatagt
     1261 gccggcgaag gtgccggcac aggcgccagc gggaggacca tcatggcgct tgcatcagcc
     1321 atcgtcacgg ccaccgattt tgcccgccac caggcgcggc tccgccccgc ggcgccggcc
     1381 ttctggttcg aaagccagga aaccagtttc cgggaactcg acatgcggtc cagccagtgc
     1441 gcccaggcgc tgctggctgc cggtgttcgg ccgggcgaac gcatcgccgt tctgagcaag
     1501 aacaacgacg ccttcccggt gctgtggttc ggcgcgatga aggcgcgcgc ctgcacggtg
     1561 ccagtgaaca tccgtctggc gccgcccgag atcgccgcga tcctgcgcga cagcgacgcc
     1621 acgcacctgc tggtgggcca cgacttcgcc gcgctggccg agcagatcgc agagcaatgc
     1681 ccgatgctga agaccgtgtg ccaattcgaa cccgggcatc cgcggcttgc gggctttgat
     1741 tcctggattg cgccgtttcc gccggaggat ccgtgcctgc ccgagcagcc ggacgacgac
     1801 atcattcagc tttacacctc cggcaccacc ggcctgccga aaggcgtgcc gctgacccac
     1861 cggaactgca tggcgcaatg cgagtccggg acgcagcaga gctatgcaca atggcaaccg
     1921 gagaaatcca gcctgctcgc cctgccggtg ttccacattg ccggcgcgct ggtggcgctg
     1981 ttgtcggtgt tgcatggcac gcgcgcggtg atggtgcggg agatcgtgcc ggcggaactg
     2041 gcgcgcgtca tcgcggaaca gcgggtcgcc tttgcctttc tgacgccgac cgtgatccag
     2101 atgatcctgt cggcaccgga aagcgcgaac gccgattact cggcactgga gcacgtgttc
     2161 tacggcgcgt cgccgatctc ggaagcgctg ctgcggcggg cgatggcgcg cctgccgtgc
     2221 cagttcaccc aggtctacgg catgacggaa gcgaccggcg tggtgacggc gctgccgccg
     2281 gaaaagcacg ttcccggcaa gctgctgtcc tgcggcgcgc cgatcgcggg cgtggagttg
     2341 cgcgtgatcg atgcggaggg gcgcgatgtg ccgcagggcc aggttggcga ggtggcgcta
     2401 cgcgcgcctt tcatcatgcg cggctattgg aagcagccgg aagccacggc ggcggcgatc
     2461 gacgccaatg gctggtatcg cagcggcgat gccggctggg tggacgccga cggcgatctg
     2521 ttcatccatg accgggtgaa ggacatgatc gtgtccggcg gagagaacgt ctatccggcc
     2581 gaggtggaaa acgcgatcga cgggcatccc gatgtggcgg aggtcgcggt gatcggcgtg
     2641 ccggacgaac gctggggcga ggcggtgaag gcgatcattg tgccgaagcc cggcgcgccg
     2701 cgcgacgcgg gttcgatcat cgcctgggcg cgccggcgga ttggcggatt caaggtgccg
     2761 aaatcggtgg atttcgccga ggcgctgccc cgcaatgcta cgggcaaggt gctgcggcgg
     2821 atgctgcgcg atcaatactg gacgggcagc acgcggcgcg tcggctagcg agcacgccgc
     2881 attctcgtgt catggtggga cgcggcagca tcaggccggc ggtttgccgt ctgtccccca
     2941 ggcgatggca ttgcgcagca gttgcgtgaa
//
LOCUS       DASSZH010000143        10929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_139772, whole genome shotgun sequence.
ACCESSION   DASSZH010000143 DASSZH010000000
VERSION     DASSZH010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 10929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10929
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139772"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..959)
                     /locus_tag="VFN42_03005"
                     /pseudo
     CDS             complement(134..959)
                     /locus_tag="VFN42_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611868.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            1144..1398
                     /locus_tag="VFN42_03010"
     CDS             1144..1398
                     /locus_tag="VFN42_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET8995614.1"
                     /translation="MTDNEILGALNAIFRHVLDDPSIALTPSTTADDVPGWDSMTQIT
                     LAVEIEHALGVKFKTSEMEEMRNIGDLVALVHTHLTAVAG"
     gene            1420..3033
                     /locus_tag="VFN42_03015"
     CDS             1420..3033
                     /locus_tag="VFN42_03015"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HET8995615.1"
                     /translation="MLFNSYVFLFLFLPLALAGYHVAGRFGRRPAAAWLAIMSLVFYA
                     WWDASFVLLLLISIAFNFGCSELITRLAHRPVRQAACLAAGIAANIAALVYFKYIAAL
                     ISFADAIPVLHMPVPDIILPLGISFFTFTQIGYLVDVKQGTAKDRGPLNYVLFVTFFP
                     HLIAGPILHNREMMPQFENPATFRFSSANISAGLSLFIIGLGKKCLLADPFSEAVRIG
                     FGNAAHAPLFSAWYTALAYSLQLYFDFSGYSDMAIGLARMFNVQFPLNFNSPYKATSV
                     IDFWQRWHMTLTRYLTLYVYTPVAMMISRRRAARGLGITRVAQSTPGGFATMVAFPVF
                     ITMLLAGIWHGAGLQFLVFGALHAVFLTVNHAWRLFRPRRANAPAAEANMHLPGSLLL
                     TFLCALVARVFFRAASVSAAVALLGGMVGLHGIDAVPVPGALLVPLGHLGDTLLAQGA
                     VVAAKPTDVVALILQMAWLVALYGAVFALPNSQQIMARHWPAFGVRHLGPSWIAWQPG
                     RAWGVALGMVAAVSLLAIGGTAEFLYFQF"
     gene            3107..4240
                     /locus_tag="VFN42_03020"
     CDS             3107..4240
                     /locus_tag="VFN42_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995616.1"
                     /translation="MRRAQAEGGAATAPYARFLVSWATAFVVTFVAIWLYVATCPMAF
                     LDRDYPLWRAKRAMLDQCRAGTVAVFGDSRAVAGIMPGVMPLRVTNFALSGTSPIETY
                     FAVRRALRCPTPPRLVVIAHGALKFSADADYWRFGARTGFFRYDDMRRVDGDAARLHD
                     DGIERLTADDHLQPWLRDRLYAVRLPMFYFSSLLHGYVAARWRHNLAAEEDALQSYGH
                     ALFGTAAGSSGIASEAGVDEFRASSLINLYYTRTLAMLQARHVAVVVLTMPVNQATYM
                     QSQPQFRGQFAAYLHAEAASFDNVNLSASAIGCWPDAYYGDAWHFNAVGAAVFSRAFG
                     VFLVRLLHGEPAEAPLHRCVEVPQDRGMPPGRAGRDGVPVRNG"
     gene            4296..5870
                     /locus_tag="VFN42_03025"
     CDS             4296..5870
                     /locus_tag="VFN42_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET8995617.1"
                     /translation="MLLVSEAGWTMDGPALTPVPRMQPVAFAGCFGWFHPPADGGDTA
                     VLLCPGLSQDASSGHRSYRQLADRLAAAGYPALRFDYPGTGDSCDMDGEPWAAWQDSI
                     HHALDWLHAHAGARRVVLFGLRIGAVLAALVAEHRSDVAGLVLLAPVLRGRSYVNQLA
                     VEARLRSVPSDAAGGILLGELALSEETQRLMRAVDLSAAALAQDCRVVIHAPAETPAL
                     SGCVTAWRGRGIEVACNGFDGLEALLRPTQQADAPAADFSAVLAWMRQAFPWRPARSG
                     AVPPPAYPALTPPSCLETALSFGDDGHLCGIFCRPATGAAPGLAVVMANSGGNPHHGF
                     ARFSVELARHLARAGIASLRIDFAGLGDSRTAREHDGPTHVFDVDRTGDMRAAVDLLQ
                     DLGCRRFAAFGLCSGAYHTLHAGLADPRIATLLMVNLPWFTLRHETPGPASNARRFID
                     TLAQTGTRTLFLFSEADAGLKQMERHFGSRGAALGTMPGASVVIRAGWDHELTAPGMR
                     RDAAACLLSFLQVSPG"
     gene            complement(5910..7193)
                     /locus_tag="VFN42_03030"
     CDS             complement(5910..7193)
                     /locus_tag="VFN42_03030"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET8995618.1"
                     /translation="MLACGGVAGFSLAAAAQNIQTYFPAGNAGYDQQLGVTVLTRLRP
                     LYETPGVRVGSFVISPNLDESLFYNSNLNGVPGSGSWGMRTSGAVSASSDWVRNSLAA
                     TIGFASNRFFTFPNENFVDWNVGLAGGYTINDSQLQAAYSHQTSHMLGTTIATTSSQA
                     PVLNQTDSGDLTYTFHLGDLAIIPDVNVSAYRFGPAIINGRTFDQSFLNRNVLAGGVD
                     ARYSLSEEGSVLVVLRGATSDYVQPVPGQPSNNSYGLQMLAGIDYQGEGVWRYRLLLG
                     MQRQAFQAAQYASQVAPVTEAGVIWTPTGLTTVTGNLSREIEDPTSAGTIGFVATRAS
                     LVVDHELLPNVFLQARGSDTYVQYLLSGGDTQNNASVGAGATWLLNRNMRLALNYDFT
                     WQTAIAGVAGVAQSNPLVTGAFTQHLVMLTLHLNL"
     gene            7837..8799
                     /locus_tag="VFN42_03035"
     CDS             7837..8799
                     /locus_tag="VFN42_03035"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF016120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HET8995619.1"
                     /translation="MIWPPRPLPDGGRNGDEIVVALINNMSPRSMRTTEQQFRDLLDA
                     AAGDSAVRLRLFTIHEDRRIGPAQTAERDAYQSLEALWQTRPDGMIVTGMEPTAGKLS
                     DEPAWMNLTKLINWATFRGIPTVWSCLAAHAAVLYLDDVDRVPLPRKLSGLFSCTLAV
                     PDHPLAAGFPTRWLCPHSRHNGLPEDALRARGYEIISASDEAGVDMFIRDHGAPFLFC
                     QGHPEYGPDVLMREYIRDLKRYLDGERDAPADVPARYVDAGTESRLRAIRESAPRLQG
                     TDVLARTQEVARSAVYIAGWRPVAVEVYRNWLALIAAHRREMAA"
     gene            8923..9561
                     /locus_tag="VFN42_03040"
     CDS             8923..9561
                     /locus_tag="VFN42_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995620.1"
                     /translation="MDALVKPAFKSTIEEIDRLDWDSLDRKALTRIAWAYYYFSIQFR
                     ENLEAATWLYPDDEKLAHLKSEECDTANLSPWPGVAKPGERMNHDEFMRRLLDLDALD
                     ADARRALQGLGEAYLMDVRPYDIAAKAMSIASYEDGGLETVFRAILRARAWNSPLLQA
                     FRHFLTEHIRFDSDPDQGHGALSRHIEPDDRILPFWRKFHQLLVAAAPQLAG"
     gene            complement(9754..9921)
                     /locus_tag="VFN42_03045"
     CDS             complement(9754..9921)
                     /locus_tag="VFN42_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995621.1"
                     /translation="MAAPPLSIPPLHTLPATAEKLGVSVRLLPIPVATASRNSCRPSG
                     LPKAAAVRWRT"
     gene            10460..>10929
                     /locus_tag="VFN42_03050"
     CDS             10460..>10929
                     /locus_tag="VFN42_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET8995622.1"
                     /translation="MAQVAPGIFVRQGVTEDASRTNDDAIANIGFVVGHDAVAVFDPG
                     GSLRDGECLRLAIRRETSLPIRYVILSHVHPDHIFGAAAFAAENPVFVGHARLPAALA
                     ARGDYYRKRLDEVLGAGRAGPVVVPTLLVHDRMELNIGGRMLLLTAHDPAHTDC"
BASE COUNT         1691 a         3677 c         3604 g         1957 t
ORIGIN      
        1 gaatcctgag ccgccgttaa tcgggatgcc cgctcggtcc agcacgtcct tcgcgaggca
       61 ctccgcagca aacccgatca aatgccctgc attctgaaag cgggcgattg atgctagggc
      121 gtgtctgcaa aggctatagc cagagcagga tgaagctgat gaagaccatg gcgaggtaat
      181 tggcggcgcg cttttcgtag cgtgtggcga cgcggcgcgc ctgcttgagg cggttgatca
      241 agcgttcgat ggcattgcgg cggcgatagg cggcgcggtc gaagttgggt tgcggcgctg
      301 gtcgctcttg gtcgggatga ccgggcggat gccgcgtcgg cgcagccgtt tgcgggccgt
      361 tgggctgcta tagcctttgt cgcccgccac ccgccggggc cgcagccgcg gccggccagg
      421 gccgggacgc cggatccgct ccctggtcga gcaccgcctc cagcgcaatc tgctcgtgcc
      481 gctcgccgcc ggtcagcacg gccgtcatcg gcttgccgcc accctcggca cgcagatgca
      541 gcttggtcga gaacccgcct tggctgcggc ccagcgcctc agcctgcgcc cgaccccccg
      601 atggcgccgg gctggcggcg cgcgccagcg gcgtgttggt gggcacgaac gatggtggca
      661 tcgacgaagt gcaggtccca gtcgatctcg ccacgggcgt cggcgtcggc ctgcaagccg
      721 gccagcacgc gctgccatat cccgccggcg cgccagcggt agaaccggct cgtcaccgtc
      781 cccaccgggc cgtagcgccg cggcaggtcg cgccacggcg cgcccgtacg gtgaacccac
      841 aggatgccgt tcataatgcg ccggtggtcc tcgttcggcc gtcccgtgcg cggcttttca
      901 gggggcagca gcttctccag ccgctcccac tgtgcgtcag tcagttcacc tcgtctcatg
      961 tcccatagct gggatcacag tcccgaaacg tcatcccttt gcagacacgc cctagccgaa
     1021 tggacgaact gacagcaaag gtcgaacccc gcataaacct atcccgtggt cgcccgtgct
     1081 gcgcagccgc atgctccggc gcgtcgcgac gcaaggccac atgacatccc gaaaggcccg
     1141 gcggtgacag acaacgaaat ccttggtgct ctgaatgcaa tcttccggca tgtgctggat
     1201 gatccatcca tcgcgctgac gccgtccact acggccgacg acgttcccgg atgggactcg
     1261 atgacgcaga tcacgctcgc ggttgagatc gaacatgcgt taggcgtgaa gttcaagacg
     1321 tcggagatgg aggagatgcg caacatcggg gatctggtgg cgctggtgca cacgcatctc
     1381 acagccgtcg ccggatagca gcggccggcc acgcggacga tgctgttcaa ttcatacgtc
     1441 tttttgttcc tcttcctgcc gctggcgctg gccggctacc atgtcgcggg gcggttcggc
     1501 cggcggcctg cagcggcctg gctcgccatc atgtcgcttg ttttctatgc ctggtgggac
     1561 gcaagcttcg tcttgctgct tctgatctcg atcgcgttca acttcggttg ctcggagctg
     1621 atcacgcgtc tcgcgcaccg gccagtacga caggccgcat gccttgcagc cggcatcgcc
     1681 gcgaacatcg ccgcactcgt ctatttcaaa tacatcgcgg cgctgatctc cttcgccgat
     1741 gcgatcccgg tcctgcacat gccggtgccg gacatcatcc tgccgcttgg catctcgttc
     1801 ttcaccttca cgcagatcgg ctatctggtg gacgtgaagc agggaacggc gaaagaccgc
     1861 ggcccgctga actacgtcct gttcgttacc ttcttcccgc acctgatcgc cggtccgatt
     1921 ctgcacaacc gcgagatgat gccgcagttc gagaacccgg cgacattccg gttctccagc
     1981 gcgaatatct cggccggact atcgctgttc atcatcggac tcggcaagaa atgcctgctt
     2041 gccgacccat tttccgaagc cgtccggatc ggcttcggca acgccgcgca cgctccgctg
     2101 ttctcggcat ggtacaccgc gctggcatac agcctgcaac tctacttcga tttctccggc
     2161 tactcggaca tggccatcgg acttgcccgc atgttcaacg tgcagttccc gctcaacttc
     2221 aactcaccct acaaggccac cagcgtcatt gacttctggc agcgctggca catgacgctg
     2281 acgcgctacc tgacgctcta tgtctacacg ccggtcgcca tgatgatttc gcgcaggcgg
     2341 gcggcacgcg gcctcggcat cacgcgggtg gcacagtcca cgcccggggg gttcgcgacg
     2401 atggtcgcct ttccggtatt cattacgatg ctgctcgcgg gcatctggca tggcgccggg
     2461 ttgcagttcc tggtgttcgg cgcgctgcat gcggtgttcc tcaccgtcaa tcatgcctgg
     2521 cgcctgttcc ggccacgccg ggcgaacgcg ccggcagctg aagcaaacat gcatctgccg
     2581 ggtagcctgc tgctgacctt tctgtgcgcg ctggtggcac gggtgttctt ccgcgccgct
     2641 tccgtctccg ccgccgtggc gctgctgggc ggaatggtcg ggctgcacgg catcgatgcc
     2701 gtgccggtgc cgggcgcgct gctggtgccg ctcggccatc tcggggacac gctgctggca
     2761 cagggcgccg tcgtcgccgc caagccgacc gatgtggtgg cgttgatcct gcagatggcc
     2821 tggctggtgg cactttatgg ggcggttttc gcgttgccca attcgcagca gatcatggcc
     2881 cggcactggc ccgcgttcgg cgtgcggcac ctgggaccat cctggatcgc ctggcagccg
     2941 ggtcgtgcct ggggagtcgc cctgggcatg gtggcggcgg tcagcctgct cgccatcggc
     3001 ggcaccgccg agttcctgta cttccagttc taggcagcga ggtttgtgca tgacggaggg
     3061 gggcgcttca tcgttggcaa cggcgggtgc gccggccggg cagacaatgc ggcgcgcgca
     3121 ggccgagggt ggcgcggcca cggcgcccta cgcgcgcttc cttgtgtcct gggccaccgc
     3181 gttcgtggtg acgttcgtcg cgatctggct ttatgtcgcg acctgcccaa tggcgttcct
     3241 cgaccgcgac tatccgctct ggcgcgccaa gcgcgcgatg ctcgaccagt gtcgcgcggg
     3301 aacggtcgct gtgttcggtg acagccgcgc cgtcgccggc atcatgcccg gcgtgatgcc
     3361 gcttcgggtg acgaatttcg cgctttccgg caccagcccg atcgagacct attttgcggt
     3421 acgccgcgcg ctgcgctgcc cgacgccgcc ccggctcgtc gtgatcgccc atggcgcgct
     3481 gaagttcagc gccgacgccg actactggcg tttcggtgcg cgaaccgggt tcttccgcta
     3541 cgacgacatg cgccgcgtgg acggcgacgc cgcacggctg cacgacgacg gcattgagcg
     3601 gctgacggcc gacgaccatc tgcagccttg gcttcgcgac aggctctatg cggtgcgcct
     3661 gccgatgttc tatttcagca gcctgctgca cggttatgtt gcggcacgct ggcgccataa
     3721 cctggccgcg gaggaggacg ctttgcagtc ctacggacac gccctgttcg gcaccgccgc
     3781 cggatcgagc ggcatcgcca gcgaagctgg cgtggacgaa ttcagggcct cgtcgctgat
     3841 caacctctat tacacgcgca cgctcgcaat gctgcaagcg cggcacgtgg cggtggtcgt
     3901 gctgaccatg ccggtcaacc aggcgacata catgcagtcg cagccgcaat ttcgcgggca
     3961 gttcgcggcc tatctgcacg ctgaagcagc cagcttcgac aacgtcaacc tgtccgcctc
     4021 ggccatcggc tgctggccgg acgcctatta cggcgacgcc tggcatttca acgccgtggg
     4081 tgccgccgtc ttcagccgcg cgttcggtgt cttcctcgtg cgcctgctgc atggcgagcc
     4141 ggcggaggcg ccgctgcacc gctgcgtcga ggtgccgcag gatcgtggca tgccaccggg
     4201 ccgggccggg cgggacggtg tccctgttcg aaatgggtaa cggggcgccg ccggtcgtcg
     4261 cctatgatgc cgcccgcgag agggaaactg ccgtcatgct gcttgtgtcc gaagccggct
     4321 ggacgatgga cggaccggcg cttacccccg tgccgcgcat gcagccggtc gccttcgccg
     4381 gctgcttcgg ctggttccat ccgccggcgg acggcggcga cacggcggtg cttctctgtc
     4441 cggggttgag ccaggatgcc agcagcggcc accgttccta ccgccagctt gccgacaggc
     4501 tcgccgcggc gggctatccg gcgctgcgct tcgactatcc cggcaccggc gattcctgcg
     4561 acatggacgg cgagccctgg gcggcctggc aggacagcat ccatcacgcg ctggactggc
     4621 tgcacgccca tgccggtgcg cgtcgggtgg tgctgttcgg gctgcgcatc ggcgccgtgc
     4681 tcgccgcgct ggtagcggag caccgcagcg acgtggcagg cctggtgctg ctggcgccgg
     4741 ttctgcgggg caggagctat gtcaaccagc tcgcagtcga ggcgcggctg cgttccgtgc
     4801 catccgacgc ggccggcggc atcctgctcg gcgaactcgc gctgagcgag gagacgcagc
     4861 ggctgatgcg ggcggtggac ctctcggccg ccgcgctcgc gcaggattgc cgggtggtga
     4921 tccatgcccc ggcggagacg ccggcgctgt cgggctgcgt gaccgcttgg cgggggcggg
     4981 ggatcgaggt tgcctgcaac ggcttcgacg gcctggaggc actgctgcgg cccacgcagc
     5041 aggctgacgc gccggccgcc gatttctccg ccgtgctggc atggatgcgg caggcgtttc
     5101 cgtggcgccc ggccaggtcg ggcgcggtcc cgccgcccgc gtatcccgcg cttactcccc
     5161 ccagctgcct ggaaaccgcg ctgagcttcg gcgatgacgg gcatctctgc gggatcttct
     5221 gccgcccggc gaccggcgcc gcgcccggcc ttgcggtggt aatggccaat tcgggcggca
     5281 acccgcacca cggcttcgcc cgcttttcgg tcgagctcgc gcggcatctc gcgcgcgccg
     5341 gtatcgcgtc gctgcggatc gatttcgccg ggctgggcga cagccgtacc gcccgcgagc
     5401 atgacggccc gacgcatgtg ttcgacgtgg accgcaccgg cgacatgcgc gccgccgtag
     5461 acctgctgca ggacctgggc tgccgccgct tcgccgcctt cggcctttgc tccggcgcct
     5521 accacaccct tcacgcgggc ctggccgacc cacggatcgc cacgctgctg atggtcaacc
     5581 tgccatggtt cacgttacgc cacgagacgc ccggtccagc cagcaacgca cgccgcttca
     5641 tcgacacgct ggcgcagacc ggtacccgga cgctgttcct gttctccgaa gccgatgcag
     5701 ggctcaagca aatggagcga cacttcggct cgcgcggggc ggccctgggc accatgcccg
     5761 gtgcgtcagt ggtgatccgg gcaggctggg accacgagct caccgcgcca ggtatgcgac
     5821 gggacgccgc cgcctgcctg ctgtcgttcc tgcaggtgtc gccgggctga gcacccgtgc
     5881 gtcgcttgcg cgaaatccca ggtgcgtgcc tataggttca gatgcagggt cagcatcacg
     5941 agatgctggg taaaggcgcc ggtgacaagc gggttcgact gcgccactcc ggccacgccg
     6001 gcgatcgcgg tttgccaggt gaagtcgtag ttgagggcaa gccgcatgtt ccggttcagc
     6061 agccaggtcg cgcccgcgcc aacgcttgcg ttgttctggg tgtcgccgcc gctcagaagg
     6121 tactggacgt aggtgtcgct gccgcgcgcc tggaggaaga cgttgggcag cagttcgtga
     6181 tcgacaacca ggctcgcccg cgtcgcgacg aacccgatcg taccggcgga cgtcggatcc
     6241 tcgatttcac gcgataagtt gccggtcacg gttgtcaggc cggtaggcgt ccagattacg
     6301 ccggcctccg tcactggggc cacctggctg gcatactgcg ccgcctggaa cgcttgcctc
     6361 tgcatcccca acagcaggcg atagcgccac acaccctcgc cctggtagtc gatccccgcc
     6421 agcatttgga gcccgtagga attgttgctg ggctgccccg gcaccggctg gacgtagtcg
     6481 gaggtggcgc cgcgcagcac gaccagtaca ctgccctcct cgctcaatga gtagcgtgcg
     6541 tcgacgccgc cggcaagtac gttgcggtta aggaaactct ggtcgaacgt gcggccgttt
     6601 atgatggccg ggccgaaacg ataggcgctg acgttgacat ccgggatgat cgcgaggtcg
     6661 ccaagatgaa acgtataggt caggtcgccg ctatcggtct ggttcagcac cggcgcctgc
     6721 gagctcgtcg ttgcgatcgt ggtgccaagc atatgcgacg tctggtgcga ataggcagcc
     6781 tgcagctggc tgtcgttgat cgtgtagccg ccggccagcc cgacattcca gtcgacgaaa
     6841 ttctcgttgg ggaaggtgaa gaaacggttg ctcgcaaagc cgatggtggc cgccaggctg
     6901 ttgcgcaccc agtcggagct cgcgctgacc gcgcccgacg tgcgcatccc ccagctgccc
     6961 gatcccggta cgccgttgag gttggaatta tagaacagcg attcgtcgag attcggagag
     7021 atgacgaaac tgccgacgcg cacgcccggc gtttcataga ggggacgcag gcgggtcagc
     7081 accgtcacgc caagttgctg gtcgtatccc gcgttgcccg ccgggaaata ggtctggata
     7141 ttctgggcgg ccgccgccag tgaaaatccc gcgaccccgc cgcacgccag cacggcccgc
     7201 aataccgcaa agcgccagcg cgccgccgtt ctgcgacgcc cgcccctcat gtgacacggc
     7261 gcgcgagcgg attccttaca tccgttccgc gccagccatc acacgacccg gttgcccctg
     7321 tccgtcggcg attccctcca tgcagccgca gtgcctgaca catgtcccat cacctgcgca
     7381 tccacgcgcg atattccggc atcgttcacg cggagtcaaa gccgacgtgt cctgccggtg
     7441 cctgccctgc cgacacccgc gccacccgct gcgtgctcat cccgtaccat ccaaacctca
     7501 aggcacgggg cgcagaaaat cgtccaatcg gcgcatgaca cgcgtgtcgg acggccatac
     7561 ttttggctga attgccgcac ggaccgccga cgtagtctga tgccatccag cagtgtccga
     7621 ggccgcttcc gcggcaaggt catcaagcga cgccactcac cgtacgtttc cgatgtctct
     7681 cacgatgtgc cgtgtccaca cctcgcttcc cgtctcgggt ggtcatgcca tggacgaatc
     7741 gccgtacacc actgctgctt tctccggttg gtcaggtgcg aagcgatgcc ggcaccttca
     7801 cggcgacagc agcccgcagc gggagctgac acgtcaatga tctggccccc acgcccgttg
     7861 ccggacggcg gccgcaatgg cgacgagatc gtcgtcgccc tcatcaacaa tatgtcgccc
     7921 cgctcaatgc ggaccaccga acaacagttc cgcgatctgc tggacgcagc ggccggtgac
     7981 agcgcggtca ggctgaggtt gttcacgatt cacgaagacc gacggatcgg cccggcgcag
     8041 acggcggagc gcgatgccta ccagtcgctt gaagcgcttt ggcagacccg gccggacggc
     8101 atgatcgtga ccggcatgga acccactgcc ggaaagctgt ccgatgagcc ggcatggatg
     8161 aacttgacga agctgatcaa ctgggcgacg ttccgcggca tcccgaccgt atggtcctgc
     8221 cttgccgcgc atgctgccgt cctgtatctc gatgacgtcg accgggtacc gctgccgcgc
     8281 aagctctccg gcctgttctc ctgcaccctg gccgtcccgg accacccact agcggcaggc
     8341 tttccgaccc gctggctctg cccccattcg cgacacaacg gcctgcccga agatgccctt
     8401 cgcgcaaggg gctatgagat catctcggct tccgacgaag cgggcgtcga catgtttatc
     8461 cgcgatcatg gcgcgccgtt cctgttctgt cagggccatc ccgaatacgg accggatgtc
     8521 ctgatgcggg aatacatccg cgacctgaag cggtacctcg acggcgaaag ggatgcccct
     8581 gccgacgttc ccgcccgcta tgtcgacgcc ggcaccgaat cccggctgcg cgccatccgg
     8641 gaaagcgcgc cacgcctgca gggcacggac gtccttgcca ggacgcagga ggtggcccgc
     8701 agcgccgtct acattgccgg atggcgaccc gtcgcggtcg aggtttaccg aaactggctt
     8761 gcattgatcg cggcacatcg ccgcgagatg gcggcgtagg caggtgcctt gaatcgatgc
     8821 cgtacaacgt ccggcacatg gcagacctcg cttccgaccg gattcatcgc gtgtacgacg
     8881 cctgaactgc tgacccacag gaccgccgag aaaggaagga cgatggacgc cttggtgaaa
     8941 cctgccttca aaagcacgat cgaggaaatc gatcggttgg attgggattc cctcgacagg
     9001 aaagcgttaa cccgcatcgc gtgggcgtac tactacttct ctatccaatt ccgtgaaaac
     9061 cttgaggccg cgacttggct ttacccggat gatgagaagc tggcgcacct gaagtccgag
     9121 gagtgcgaca ccgccaacct gtcaccatgg cccggtgtcg ccaagccagg tgaacggatg
     9181 aaccatgacg agttcatgcg gcgcctgctc gacctggatg ctctcgatgc cgatgcgcgc
     9241 agggcgcttc agggactggg cgaagcctac ctgatggatg tgcgtccata cgacatcgcc
     9301 gccaaggcca tgagcatcgc aagttatgag gatggcggcc tggaaacagt cttccgggcc
     9361 atcctgaggg cgcgcgcctg gaacagcccg ctcttgcagg ccttcaggca tttcctgacc
     9421 gagcacatcc gcttcgatag cgacccggat cagggtcatg gggcgctgag ccgccatatc
     9481 gaaccggatg accgcattct cccgttctgg cggaagttcc accagcttct ggtcgccgcc
     9541 gcgccccagc ttgccggctg atcccgtccc ggaccgcgat ttcgcggtat ccggcgcgag
     9601 atcgcgcggc gaaaagacag cccgcggtgg accggcgcac catcgccggt ccgttccgat
     9661 gcggtctgtt cagctcgaca atcggtcacg gtgcatgaac ggacggtggg caagctgccg
     9721 ccccagcttg gccggacccg gctgcagccg cgatcaggtg cgccagcgca cggcggcggc
     9781 cttcggcaag ccgctcggcc ggcagctgtt tcgcgatgcg gtggcaaccg ggatcgggag
     9841 caggcggacg gaaacgccga gcttctcggc ggtggcggga agggtgtgca gcggcgggat
     9901 gctcagcggc ggcgcggcca aggcgatatc ctctgatgac gggaggggat ctgtgccggt
     9961 ggatcggatg gccgggcatt cgccggccgt ggcgccgtgg gcgcaggacg agcaccgcca
    10021 cgccggtcgg gtaaccggca tcatgcgcag gatcggggat gcgggcctgg ggtgctcatc
    10081 ccgcaagcct gccctcacga cctgaccggc ccgcctgcgg acagcggtca gggggtggga
    10141 gagaaaagtc ccgatatcag ggcgcgaggc gtgccgtcgt gggcgcctcc gtcctggcgt
    10201 tgtcgcgctc tccgtcgagc ctctgtagaa cgtccgtagt ggctttgtag tggcgctgta
    10261 gcgcccgtgg gcggaacgga tgtgtgacag accaagtgac ccatcgggtc gtgcagggga
    10321 gcgttggcac gccgctgcgg aggtgcgagg attgatgttg tcgcgccggc ggattgccct
    10381 gagcggtctg tgcctgtgct gcctgccatc gccggcacgg caggccgcag cggcggcttc
    10441 ggccgtgggg ccggccgaga tggcccaggt cgcaccgggg attttcgtac gccagggcgt
    10501 gacggaggat gccagccgga ccaatgacga cgccatcgcg aatatcggct tcgtcgtcgg
    10561 gcacgacgcc gtggccgtgt tcgatccggg tggcagcctg cgtgacggcg agtgcctgcg
    10621 cctcgccatc aggcgggaaa ccagcctgcc catccgctac gtgatcctgt cgcatgtgca
    10681 tccggaccat atcttcggcg ccgcggcgtt cgccgccgag aacccggtct tcgtcggtca
    10741 tgcgcgcctg ccggcggcgc tggccgcgcg tggcgactac taccgcaaaa ggctcgacga
    10801 ggttctcggc gccggacgcg cggggccggt cgtggtgccg accctgctgg tgcatgaccg
    10861 gatggagctg aatatcggcg gtcgcatgct gctgctgacg gcgcatgatc ccgcccatac
    10921 cgattgcga
//
LOCUS       DASSZH010000144         3150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_54536, whole genome shotgun sequence.
ACCESSION   DASSZH010000144 DASSZH010000000
VERSION     DASSZH010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54536"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VFN42_03055"
     CDS             complement(<1..282)
                     /locus_tag="VFN42_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995623.1"
                     /translation="MPEDHAHRAPLIDQQTPPRPKAVRPRHAASLVVYRQEGARLTML
                     MGMRGAKHRFMPNRLVFPGGAVDRADLTTPSATKLAPQTEHLLRKSANEK"
     gene            complement(275..1099)
                     /locus_tag="VFN42_03060"
     CDS             complement(275..1099)
                     /locus_tag="VFN42_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET8995624.1"
                     /translation="MADGAELLRARFGIDVPGAEVPDAIAPLLDRRVVRRYRDEPVPD
                     SLLDALLAAAQSAPAKSDLQQYSVVVMRDPARIKRIADWIGTMDWIATAPVFLVWCGD
                     MRRGQRLCEQHAMPHANNNLDTFLNTAVDCSLAMGQFMAAADAVGLGTCPISYVRSHI
                     EKVTPLLGLPAGVYPVAGLTVGWPVFRRPVSMRLPPAVVVHRERYDDSNLQADVQGYD
                     ERRRTRDPVAAGSLKNNDVYPPRDGVGWSENVARQLSVPERFGFAAYLKTRGFDLA"
     gene            1241..1561
                     /locus_tag="VFN42_03065"
     CDS             1241..1561
                     /locus_tag="VFN42_03065"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET8995625.1"
                     /translation="MHSVVTAKRHAIVELCRRFHVRRLEVFGSAARSTDFDPQRSDTD
                     FMIEFEPGQDPGMAGFLHLKQALEQTVGRPVDLVDRQAVERSRNYLRRRQILGEAEPV
                     YVAG"
     gene            1548..1895
                     /locus_tag="VFN42_03070"
     CDS             1548..1895
                     /locus_tag="VFN42_03070"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HET8995626.1"
                     /translation="MLRDETLLLDMLLAARDAIGFVAAIDEAQFEDSKLHQNAVIRSI
                     EIIGEAAGKVSRAFQQVHPEFPGMAIIGMRHRLIHDYNEVRLDLVWDVARNRLPELIT
                     ALQVLVPPPDDEG"
     gene            1967..3031
                     /locus_tag="VFN42_03075"
     CDS             1967..3031
                     /locus_tag="VFN42_03075"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HET8995627.1"
                     /translation="MPAPYEKHRAQLKAILLAWGMPEDNAEITADVLSWADLHGVDSH
                     GMSMIPGYDGLRRRGRAKMDARPRIVKQTPVSALVDGGGGLGHVPAHFAMSVAIDKAK
                     QSGMAIAAVNNSAHFGATGYYTLMAAQAGLIGMACTSASGIQVAPTFGKQARLGTDPW
                     SFAAPTTDGRPFLLDMATTTVAAGRIRNKANEGLPTPDGWVLDSEGRPSNDPLVAREK
                     GGFLTSLGGSPENSSYKGYGLAAMVNILASCLSGATLITDPMHTKKPVGMDIGHFFMA
                     IDPGIFRETDDFQQDVASFLGALRGTTPVDPAQPVMVAGDPQWKNAEARMKNGIPVGP
                     GLLNQVRQIAQAAAAPWLLD"
BASE COUNT          507 a         1022 c         1109 g          512 t
ORIGIN      
        1 cttctcgttg gcgctcttgc gcagcaggtg ttccgtctgc ggcgccagct tcgtggcgct
       61 tggcgtggtg aggtcagcgc ggtccaccgc gccgccggga aataccaggc ggttcggcat
      121 gaagcgatgc ttcgcgccgc gcatgcccat gagcatcgtc aggcgcgcgc cttcctgccg
      181 gtacaccacc aggctggccg catggcgggg acgcacggct ttcggacgcg gcggcgtctg
      241 ctggtcgatc agcggcgcgc ggtgcgcgtg gtcttcaggc aagatcgaaa cctctcgtct
      301 tgagataggc ggcgaagcca aagcgctccg gcaccgacag ctggcgggca acgttctcac
      361 tccagcctac gccgtcgcgc ggcggataca cgtcgttgtt cttcaggctg ccggcagcga
      421 ccggatcgcg ggtgcggcgg cgttcgtcat agccctgcac gtcggcctgc agattgctgt
      481 cgtcgtagcg ttcgcgatgc actaccacgg cgggcggcag gcgcatcgat accgggcggc
      541 ggaacaccgg ccagccgaca gtgagccctg ccaccggata cacgccggcg ggaagcccga
      601 gcagcggggt gaccttctcg atatggctgc gcacgtagct gatcggacag gtgcccaggc
      661 cgaccgcgtc cgccgccgcc atgaactggc ccatcgccag gctgcaatcc accgccgtgt
      721 tgaggaaggt gtccaggttg ttgttggcgt gcggcatggc gtgctgctcg cacaggcgct
      781 ggccgcggcg catgtcgccg caccacacca ggaacaccgg cgccgtggcg atccagtcca
      841 tggtgccgat ccagtcggcg atgcgcttga tgcgcgcggg gtcgcgcatc accacgacgc
      901 tgtattgctg caggtcggac ttggccggcg ccgattgcgc ggcggcgagc agcgcatcga
      961 gcagcgagtc cggcaccggc tcgtcgcggt agcggcggac cacgcgacgg tccagcagcg
     1021 gggcgatggc gtccggcacc tcggcgcccg gcacgtcgat gccgaagcgt gcccgcagca
     1081 attcggctcc atcggccatg acgcactctc ccgttctgtc tggatcagcc ttgccggaat
     1141 ggccggaccg cgcaagccgc gccgagcggc cgcggtcagg ctgcattggc aggtccgtga
     1201 tgctatatag gccgcaatcg gacatcctca ggcagggtcc atgcacagcg tcgtcaccgc
     1261 caagcgccat gcgatcgttg aactctgccg ccgcttccat gtccggcggc tggaggtatt
     1321 cggttctgct gcccgttcga ccgatttcga tccccaacgc agcgacaccg acttcatgat
     1381 cgaattcgaa ccgggccagg atccggggat ggccggtttt ctgcacttga agcaggctct
     1441 ggagcagacc gtcgggcggc ccgtcgatct tgtcgatcgg caggcggtgg agcgcagccg
     1501 caactacctg cgccggcggc agatcctggg cgaggcggag cccgtctatg ttgcgggatg
     1561 aaacgctgct gctggacatg ctgttggcgg cccgtgatgc gattgggttt gtcgctgcga
     1621 tcgacgaggc gcagttcgaa gacagcaagc tgcaccagaa cgccgtcatc cgcagcatcg
     1681 agatcatcgg cgaagcggcc ggcaaggttt cgcgcgcgtt ccagcaagtc catccggagt
     1741 tcccggggat ggcgatcatt gggatgcgcc atcgtctgat ccatgactac aacgaggtcc
     1801 ggcttgatct ggtctgggat gtagcgcgca atcgcctgcc cgaactcatc accgcgttgc
     1861 aggtgcttgt gccgccccct gatgacgagg gctgaccctc gccgcgcggc cttgccggaa
     1921 cgcctctgcc gcgcaaccct ggcggcatat gccaaagggg acgtccatgc cggcaccgta
     1981 cgagaagcac cgcgcgcagt tgaaggccat cctgctggcc tggggcatgc cggaggacaa
     2041 cgccgagatc accgccgacg tgctgtcctg ggcggacctg cacggggtgg acagccacgg
     2101 catgtcgatg atccccggct acgacggact gcgccggcgc ggcagagcga agatggacgc
     2161 gcggccgcgc atcgtgaagc agacgccggt gtccgccctg gtggatggcg gcggcgggct
     2221 ggggcacgtg ccggcgcatt tcgccatgtc ggtggcgatc gacaaggcga agcagtccgg
     2281 catggcgatc gccgcggtga acaactcggc gcatttcggc gccaccggct actacacgct
     2341 gatggcggcg caggcggggc tgatcggcat ggcctgcacc agcgcctccg gcatccaggt
     2401 ggcgcccacc ttcggcaagc aggctcgtct tgggaccgac ccgtggtcct tcgccgcgcc
     2461 caccaccgac gggcggccgt tcctgctgga catggcgacc accaccgtcg ccgccgggcg
     2521 catccgcaac aaggcgaacg aggggctgcc gacgccggac ggctgggtgc tggacagcga
     2581 aggccgcccc agcaacgacc cgctggtggc acgggagaag ggcggcttcc tgacctcgct
     2641 gggcggatcg ccggagaatt cctcctacaa gggctatggg ctggcggcga tggtgaacat
     2701 cctggcctcc tgcctgtccg gcgccacgct gatcaccgat ccgatgcaca cgaaaaagcc
     2761 ggtcggcatg gatatcgggc atttctttat ggcgatcgac cctggtatct ttcgtgagac
     2821 cgatgacttc cagcaggatg tcgccagctt cctgggcgcg ctgcggggga ccacgccggt
     2881 ggatccggcg cagccggtga tggtggcggg tgatccacag tggaagaatg ctgaagcgcg
     2941 gatgaagaac ggcatcccgg tcgggccggg gctgctgaac caggtgcggc agatcgcgca
     3001 ggcggccgcc gcgccgtggc tgctggatta ggcgggtagg gagtgcgggc agatgtcgaa
     3061 gcatgcggaa gtgcggcgcg tcacggtgcc agacatccgg gggctgaagg gccggcagaa
     3121 gatcgtttgc ctgacggcct atacggcgcc
//
LOCUS       DASSZH010000145         3787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1605830, whole genome shotgun sequence.
ACCESSION   DASSZH010000145 DASSZH010000000
VERSION     DASSZH010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1605830"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="VFN42_03080"
     CDS             <1..908
                     /locus_tag="VFN42_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995628.1"
                     /translation="RARRMLMAIYRGFCVMNCWSPLNLMTAVVSTAVPEAPMRLLMPI
                     AFVVSIGMAALGWLEDRISGARRATRGGTRPVSGDSWSIHLRIVALVGLVMLLAELGS
                     DLLRVSLVTGVTLMVPIVALGWIVVQLRRHARTLSRLTLALLRRRGARFLWRVPHFRS
                     EATVLAGSGFMGVAIGGMLPAAGIAPLLAHLPAISVPLLIPVVLIATGQIGLNPVAVV
                     ALFGAAIPHPAALGVPPAALAFACMLGWGLAVNMTPMSASAITTARWARVSPWTVSTA
                     WNAAYTGSALLLAWAAIALLFLLWR"
     gene            complement(910..1509)
                     /locus_tag="VFN42_03085"
     CDS             complement(910..1509)
                     /locus_tag="VFN42_03085"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HET8995629.1"
                     /translation="MTQATEVPAALRIAVPADDLCLRFINTRYWRGMAEPTEELREPS
                     DLQRWLQAEAGLPPESVRQAGDAFADALRLRETLFRLFVALAAGKPGAAADVAALNAA
                     LATVPARTCITLSAPSGWLVPELRPAALLAPVLWSAGDLALGPRAGRVRQCANPACQW
                     LFLDDSKSGNRRWCSMSACGNRAKAHRHYLKQKGQLAAG"
     gene            1634..2008
                     /locus_tag="VFN42_03090"
     CDS             1634..2008
                     /locus_tag="VFN42_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8995630.1"
                     /translation="MLDHISLGARDIARSRRFYDAVLRPLGYTCLSTGDDSLGYGADA
                     PALWILATEHPVAADPRSGLHVCFTAPSRAAVDAFHAAALANGGRDNGAPGLRADYSD
                     SYYAAFAIDPDGYRIEAHCETA"
     gene            complement(2068..2868)
                     /locus_tag="VFN42_03095"
     CDS             complement(2068..2868)
                     /locus_tag="VFN42_03095"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET8995631.1"
                     /translation="MDFTGKVALITGAGNGIGRAAALGFAKGGAKVVAVDRDQAAGEA
                     TVGIIRQQGGDARFVAADVTQSAQVQAYVKAALDAYGAIDCFFNNAGIEGSVATTAEY
                     EEEMFDRVMAVNVKGVFLGLRHVLPVMLKQQRGAVVNTASVAGLVASPGMPAYVASKH
                     AVIGLTKTAAGEVARAGVRVNAVCPGPIDTRMIHSLESMLNPSDPTAVGTRYQANIPL
                     GRYGTAEEIANLVLFLCSDLAGNITGAQYVADGGRTATGGAVTNNLVR"
     gene            complement(2992..>3787)
                     /locus_tag="VFN42_03100"
     CDS             complement(2992..>3787)
                     /locus_tag="VFN42_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995632.1"
                     /translation="STLLAGAFAGNVLGATAYAWYNEHLYVEAGAYGTQSPWLSYRLG
                     TSGGPVSNNLMPYARAAYEWDWGSNAAWVGGTLLHANLNPMLGTGQDSYTDYAVDAGY
                     QFLGSGNHALTVQGNFVHESQHLSATAADYNVNNGTSVGADYGLNTVSVNAQYWYKET
                     YGLTLGWFAGFGSSNPLAYGTGPQLGQDLVGFANNSPNYNGFSIEADWVPFGKDKSWA
                     RPWANLKVGAEYMFYTDYNGASSNYDGFGRSASDNNVFLLTAWTVF"
BASE COUNT          533 a         1287 c         1309 g          658 t
ORIGIN      
        1 tgcgcgcccg gcggatgctg atggcgatct atcgcggttt ctgcgtgatg aactgctgga
       61 gcccgctgaa cctgatgacc gcggtagtgt ccaccgcagt gccggaggcg ccgatgcggc
      121 tgctgatgcc gatcgccttc gtggtgtcga tcggcatggc ggcgctgggc tggctggagg
      181 accggatctc cggcgcccgc cgcgcgaccc gcggcggcac gcggccggtc agcggtgatt
      241 cctggagcat ccacctgcgc attgtcgccc tggtcgggct ggtgatgctg ctggcggaac
      301 tcggcagcga cctgctgcgg gtgtcgctgg tgaccggcgt gaccctgatg gtgccgatag
      361 tggcgctggg ctggatcgtg gtgcagttgc ggcggcacgc gagaacgctc tccaggctca
      421 cgcttgccct gctccgccgc cgcggcgcgc gctttctgtg gcgcgtgcca catttccgca
      481 gcgaggccac ggtgctggcg ggttctggct tcatgggtgt ggcgatcggg ggcatgctgc
      541 ccgccgccgg cattgccccg ttgctggcgc atctgcccgc catcagcgtg ccgctgctga
      601 tcccggtggt gctgatcgcc accgggcaga tcgggctgaa tccggtcgcc gtggtggcgc
      661 tgttcggcgc cgcgatcccg catccggcgg cactgggcgt gcctccggcg gcgctggcat
      721 ttgcctgcat gctgggctgg gggctggcgg tgaacatgac gccgatgtcg gcctccgcga
      781 tcaccaccgc gcgctgggcc agggtatcgc cctggaccgt cagcacggcc tggaatgccg
      841 cctataccgg ctccgccttg ctgctggcct gggcggcgat cgccctgctg ttcctgctct
      901 ggcgctgatt caccccgccg caagctggcc cttctgcttc aggtaatgcc ggtgcgcctt
      961 ggcgcggttg ccgcaggcac tcatcgagca ccagcgccgg ttgccgcttt tgctgtcgtc
     1021 caggaacagc cactggcagg cggggttggc gcattgccgc acccggccgg cgcgcggccc
     1081 cagcgccaag tcgccggccg accacagcac cggggccagt aacgccgccg gccgcagttc
     1141 cggcaccagc catccggacg gagcactcag cgtgatgcag gtccgcgccg gcaccgtggc
     1201 cagcgctgcg ttcagcgccg cgacatccgc cgccgcgccg ggtttgccgg cggccagggc
     1261 gacgaacaac cggaacaggg tctcacgcag ccgcagcgca tcggcgaacg cgtcgccggc
     1321 ctggcgcacg gattccggcg gcagaccggc ttcggcttgc agccagcgtt gcaggtcgga
     1381 tggctcccgc agctcctcgg tcggctccgc catgccgcgc caatagcgcg tgttgatgaa
     1441 gcgcaggcaa aggtcgtccg cgggtacggc gatccgcagg gctgccggga cctcggtggc
     1501 ttgggtcatg ccgcggctta tagccgcccc cgcgatagcg ccaagccgca tcctataacc
     1561 gtcatgctgg ttgacaccgc ccgtccgtta tggcttcatc tataaccgtc aatatggtta
     1621 tggaggaacc cagatgctcg accacatttc cctcggcgcg cgcgatatcg cccgctcccg
     1681 ccggttctac gacgccgtcc tgcggccact cggctatacc tgcctgagca cgggcgacga
     1741 ttccctcggc tatggggccg acgccccggc gctgtggatc ctggccacgg agcatccggt
     1801 ggcggccgat ccgcgctccg ggctgcatgt ctgcttcacg gcgccatcgc gggcggcggt
     1861 ggacgccttc catgccgcgg cgctggccaa tggcgggcgc gacaacgggg cgccgggact
     1921 gcgcgccgat tacagcgaca gctactacgc cgccttcgcc atcgatcccg acggctaccg
     1981 gatcgaggcg cattgcgaga cggcgtgatt ccgcgccgcg tcaccgcttc cgcggcatgc
     2041 gccggacgcg gtgacgcggc gggacgttca gcgcaccagg ttgttggtca ctgcgccgcc
     2101 ggtcgcggtg cgtccgccat ccgccacgta ttgcgcgccg gtgatgttgc cggcgagatc
     2161 ggagcacagg aacagcacga gattggcgat ctcctccgcc gtgccgtagc gcccgagcgg
     2221 gatattggcc tggtagcgcg tgccgacggc ggtgggatcg gacgggttca gcatggattc
     2281 cagcgaatgg atcatgcgcg tgtcgatcgg gccgggacag accgcgttca cccgcacgcc
     2341 ggcgcgcgca acctcgcccg cggcagtctt ggtcaggccg atcaccgcgt gcttggacgc
     2401 gacataggcc ggcatgccgg gtgaggcgac caagccggcc accgaggcgg tgttcaccac
     2461 ggcgccgcgc tgctgcttca gcatcacagg cagcacgtgg cgcaagccga ggaacacgcc
     2521 cttcacgttc accgccatca cgcggtcgaa catctcctct tcgtattccg cggtggtcgc
     2581 gacggagcct tcgattccgg cattgttgaa gaagcagtcg atggcgccat aagcgtcgag
     2641 cgccgccttc acataggcct gcacctgcgc cgactgcgtc acatccgcgg cgacgaaacg
     2701 ggcatcaccg ccctgctggc ggatgatgcc gacggtggcc tcgccggctg cctgatcacg
     2761 gtccaccgcc accaccttcg cgccgccctt ggcgaagccc agtgccgccg ctcggccgat
     2821 gccgttgccg gcgccggtga tcagggcgac tttgccggtg aaatccatga cgcttcctcc
     2881 ttgttctggc tggcccagcg tagcgcggat tgccgcgcgc gggtatccgg gtgaccggcc
     2941 gcagaggcat gcgccctccc cgcccctgat ggggcgggga ggcaagcctg gtcagaacac
     3001 cgtccaggcg gtgagcagga agacgttgtt gtccgacgcg ctgcgcccga agccgtcata
     3061 gttcgagctg gcgccgttgt agtcggtgta gaacatgtac tccgcgccga ctttcaggtt
     3121 ggcccagggc cgtgcccagg acttgtcctt gccgaaggga acccagtcgg cctcgatgct
     3181 gaagccgttg tagttcggcg agttgttggc gaagccgaca agatcctggc cgagctgcgg
     3241 gccggtcccg taggcgagcg gattgctgga gccgaagccg gcaaaccacc cgagcgtcag
     3301 gccataggtt tccttgtacc agtactgggc attcacgctg accgtgttca gcccgtaatc
     3361 cgctccaacg ctggtgccgt tgttcacgtt gtagtcggcg gcggtggcgc tgagatgctg
     3421 gctctcgtgc acgaagttgc cctgcacggt cagcgcgtga ttgccactgc cgaggaactg
     3481 gtagccggca tcgaccgcgt aatcggtgta gctgtcctgc ccggtgccga gcatcgggtt
     3541 gagattggcg tgcagcaagg taccaccgac ccaggccgcg ttgctgcccc agtcccattc
     3601 ataggcggcg cgggcatagg gcatcaggtt gttgctgacc ggcccgccgg aggtaccgag
     3661 gcgataggag agccaggggc tttgcgtgcc gtatgcgccg gcttccacgt acaggtgctc
     3721 gttgtaccag gcgtatgcgg tggcgccgag cacattgccg gcgaaggcgc ccgccaacag
     3781 cgtcgaa
//
LOCUS       DASSZH010000146         4534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_88680, whole genome shotgun sequence.
ACCESSION   DASSZH010000146 DASSZH010000000
VERSION     DASSZH010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88680"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VFN42_03105"
     CDS             <1..410
                     /locus_tag="VFN42_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET8995633.1"
                     /translation="VPIEGPDRRDQFLLDGIAAQAQAYGFAARDVSPDRWLTEGESVT
                     LDEHRFDILHCPGHTPGHIVFINQAARFALVGDVLFQGSVGRTDFPYGDHAALIAAIQ
                     QKLLPLGDDMMFLCGHGPGSTFGAERETNPFLR"
     gene            612..1862
                     /locus_tag="VFN42_03110"
     CDS             612..1862
                     /locus_tag="VFN42_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006116574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HET8995634.1"
                     /translation="MFGPFERAVAGRYLRARRGERFVSIIAIFSLVGIALGVATLIIV
                     MSVMGGFKVDLLNRILGFNGHLGVYGVGGSLQNYDDLAAKIRTLPNVVSATPVLDGQV
                     LLSDSRGYSSGGYVRGITQAGLRSLPLVSSHIIAGSLDKFQGDDAIAIGGGLARKFGI
                     GVGSHLTLVSPQGAATAFGTIPRVRAYTVAAIFNVGMNEYDTLYVFLPLHAAQIFFQK
                     RDAVSQIEVRVRDPNQVGVVEQQIHQALGNTPVQVIDWQQNNNSFFNAVQVEQNVMFL
                     ILTLIILVAAFNVISSLIMMVKDKTRDIAVLRTLGAGRGAVMRIFLMCGASVGVTGTI
                     IGTIIGVVFCWNIQTIQDWVQDITGTQVFNPEVYYLTHLPARLNWHEVLQVILMALAL
                     SLLATLYPSWRAAQTDPVEALRNE"
     gene            1855..2544
                     /locus_tag="VFN42_03115"
                     /pseudo
     CDS             1855..2544
                     /locus_tag="VFN42_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007399844.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            2737..3648
                     /locus_tag="VFN42_03120"
     CDS             2737..3648
                     /locus_tag="VFN42_03120"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET8995635.1"
                     /translation="MEPELVAYSPILHRPPLRWPNGARVALWVVPNIEHYEYLPKFVR
                     TRDPWPRSPHPDILGYTQRDYGNRVGLWRFFDLADDFNLRCTVSLSMAVIQHFPAILE
                     GMEKRNWELMSHGIYNTRYHWNFSEEEERAAMLESREIHRQLTGREQRGWFSPAITNT
                     LRTFDLAAECGFTYTAELYHDDQPFPLKVKHGRLISVPYSVDLNDVILHRRGEEAEEF
                     ARQIRDYFDTVYAEGAEQGRVMCLALHPYWVGQPHRIRIVRRALEYILAHPGVWVATG
                     AEIADWFIAQHLPAVEAHLAAREAANG"
     gene            3641..>4534
                     /locus_tag="VFN42_03125"
     CDS             3641..>4534
                     /locus_tag="VFN42_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995636.1"
                     /translation="MADSTPPVNEPRLPGSDQPHAGFRILPEAPRFTWPDGARIALTV
                     TLLLDYWEIDPPQDASRDPRIISPLGNFFPDWLTWSQRQYGARVGIFRVLDVLDRFGI
                     TPSVGLGAEAAQRYPELVDELLRRNACFMAHGDFATRRLTSRMSEAQERAVIAASRDA
                     VAQVVGEAPRGWCSQDFTESANTPALLAEAGFAYTTDWANDDRPYLLGPYGGQHLVAL
                     PPQPEWNDLECMWLRRITPPVWADGITEAFSFLHEEGGGAFNLTLHPWIAGQAHRIRW
                     LREALSRVLGKPAVWRTTTDEL"
BASE COUNT          736 a         1545 c         1492 g          761 t
ORIGIN      
        1 cggtaccgat cgaaggtccg gaccgccgcg accagttcct gctggacggc atcgccgccc
       61 aggcgcaggc ctatggcttc gccgcacgcg acgtctcacc cgaccgctgg ctgacggaag
      121 gcgagtcggt gaccctggac gagcaccgct tcgacatcct gcactgcccc ggccacacgc
      181 ctggccacat cgtgttcatc aaccaggcgg cgcggttcgc gctggtcggc gacgtgctgt
      241 tccagggctc ggtgggccgc accgatttcc cctatggcga ccatgccgcc ctgatcgccg
      301 cgatccagca gaagcttctg ccgcttggcg atgacatgat gttcttgtgc ggccacggac
      361 ccggctccac cttcggcgcc gaacgcgaga ccaacccatt cctgcgatga tagcggcgcc
      421 cgccccgccg gggcggagtc gcccgccgac tggtgcgcca gcttcctcag ccggccttgc
      481 tgaacaggca gggccgccgt acataccgcg ccttcccgcc ccccggagat tgccagtgct
      541 gcgatggcgg cccttcacgc acttcgcgcc ggcgcatgca atcccggcca tgacccaggt
      601 atagccaaag catgttcgga cccttcgagc gcgccgttgc cggccgctat ctgcgcgcgc
      661 gccgcggcga gcgcttcgtc tccatcatcg caatcttctc cctggtcggc atcgccctgg
      721 gagtcgcgac gctgatcatc gtcatgagcg tgatgggcgg gttcaaggtg gatctgctga
      781 accgcatcct cgggttcaac ggtcaccttg gcgtgtacgg cgtcggcggc agcctgcaga
      841 attacgatga cctggcggcg aagatccgca cgctgcccaa cgtggtctcc gccacgccgg
      901 tgctggacgg gcaggtgctg ctgagcgaca gccgcggcta ctcctccggc ggctacgtgc
      961 gcggcatcac ccaggcggga ttgcgctctc tgccgttggt cagcagtcac atcatcgcgg
     1021 gctcgctgga caagttccag ggcgatgacg ccatcgccat cggcggcggg ctggcacgca
     1081 aattcggcat cggcgtcggc agccacctga ccctggtttc gccgcaaggc gccgcgacgg
     1141 cgttcggcac catcccgcgc gtgcgcgcct acacggtggc ggcgatcttc aatgtcggca
     1201 tgaacgaata cgacacgctg tacgtattcc tgccgctgca tgccgcgcag atcttcttcc
     1261 agaaacgcga cgccgtcagc cagatcgagg tgcgcgtgcg cgatcccaat caggtgggtg
     1321 tggttgagca gcagatccac caggcacttg gcaacacgcc cgtgcaggtg atcgactggc
     1381 agcagaacaa caacagcttt ttcaatgcgg tgcaggtgga acagaacgtc atgttcctga
     1441 tcctcacgct gatcatcctg gttgccgcgt tcaacgtgat ctccagcctg atcatgatgg
     1501 tgaaggacaa gacgcgcgat atcgcggtgc tgcgcacgct gggtgccggg cggggcgcgg
     1561 tgatgcgcat tttcctgatg tgcggcgctt cggtgggcgt gaccggcacc atcatcggca
     1621 ccatcatcgg cgtggtgttc tgctggaaca tccagacgat ccaggactgg gtgcaggaca
     1681 tcaccggcac acaggtgttc aatccggaag tctattacct gacccacctt cctgcccggc
     1741 tgaactggca tgaggtgctg caggtgatcc tgatggcgct ggcgctgtcg ctgctggcca
     1801 cgctgtatcc aagctggcgg gcggcgcaga ccgacccggt ggaggcgctg cgcaatgagt
     1861 gaacccgccc cgccgctgga attgcgtgac gtccggcgca tctaccgcac ggaagccggg
     1921 gagttgccgg tgttgcgcgg cgtcaacctg acgctgcgcg cgggcgagat cgtcgccatg
     1981 gcggcaccgt cgggtgccgg caagtccacg ctgctgcacc tggccggcct gctggagcgc
     2041 ccggatggcg gcgcggtgct ggtggaaggc cgcgacgccg gcacgctggc ggatgccgcg
     2101 cgaaccgcga tccggcgcga cacgattggc ttcgtgtacc agttccatca cctgctggcg
     2161 gagttctccg cgctggagaa cgtggtgctg ccgcagatga tcgccggcac gtcccgccgc
     2221 gatgccagcg cgcgcgcgat gcggctgctg tcggcgttcg gcctggaaag ccgggcggat
     2281 cacctgcccg gcaagctgtc cggcggctag cagcagcgcg tggcgatcgc ccgcgcactg
     2341 gcgaacgggc cgaaagtgct gctggcggat gaaccgaccg gcaacctgga tgtgcacacc
     2401 gccgacgtgg tatttgcgga attgctggcg accgtgcgcc gcgagggtgt ggccgcgctg
     2461 atcgccactc acaaccccga cctggcggcg cgcatggacc gcacggtgac cctgcgtgag
     2521 ggcatggtgg tgccgctgac gtagcgcggc gcggtgccgg caagcccgac tgggatgtcc
     2581 acccggtcgc cctctgactc tgcatccgcg ccgtaaagcc gcgccgctgg cgctatgatc
     2641 ctcgcctgac gcgcgcctcc ccctggccag cctggcggga ggattcctct ttcgctgccg
     2701 ctgctgtacg gtcgctcaac ctccagggag gcagacatgg aacccgagct tgtcgcctac
     2761 tcgcccattc tgcaccgccc gccgctgcgc tggccgaacg gagcgcgcgt cgcgctgtgg
     2821 gtggtgccga atatcgagca ctacgaatac ctgccgaaat tcgtgcgcac ccgcgatccc
     2881 tggccgcgca gcccgcatcc cgacatcctg ggctacacgc agcgcgacta cggcaaccgc
     2941 gtcgggctat ggcgcttctt cgatctcgct gacgacttca acctgcgctg caccgtcagc
     3001 ctcagcatgg cggtgatcca gcacttcccc gccattctgg aaggcatgga gaagcgcaat
     3061 tgggagctga tgtcgcacgg catctacaat acccgctatc attggaattt ctctgaagag
     3121 gaagaacgcg cggcgatgct ggaaagccgc gagatccacc ggcagctgac cggccgcgaa
     3181 cagcgcggct ggttcagccc ggcgatcacc aacacgctgc gcaccttcga cctcgcggcg
     3241 gagtgcggct tcacctacac agcggagctg taccacgacg accagccatt cccgctgaag
     3301 gtgaagcacg gacgcctgat ctccgtgccg tactcggttg atctgaacga cgtcatcctg
     3361 catcgccgtg gcgaggaggc ggaggaattc gcgcggcaga tccgcgacta cttcgacacg
     3421 gtgtatgccg agggcgcaga gcaaggacgg gtgatgtgcc tggcgctgca tccgtattgg
     3481 gtcgggcagc cgcaccgtat ccgcatcgtc cgccgagcgc tggaatacat cctggcgcac
     3541 ccgggcgtgt gggtcgccac cggtgccgaa atcgccgact ggttcattgc gcagcatctg
     3601 ccggcagtcg aggcgcatct tgcggcgcgg gaggcggcca atggctgaca gcacaccccc
     3661 cgtgaacgag ccgcgcctgc ccggcagcga tcagccgcat gccggcttcc gcattctgcc
     3721 ggaggcgcca cgcttcactt ggccggacgg cgcccgcatc gccctgaccg tcaccctgct
     3781 gctggattat tgggagatcg acccgccgca ggacgccagc cgcgacccgc gcatcatttc
     3841 accgctcggc aatttctttc cggactggct gacctggagc caacggcaat atggcgcccg
     3901 cgtcggcatc tttcgcgtgc tggatgtgct ggaccgcttc ggcatcacac ccagtgtcgg
     3961 attgggcgcc gaggcggcgc agcgctatcc ggaactggtg gacgaactgc tgcgccgcaa
     4021 tgcctgcttc atggcgcacg gcgatttcgc cacgcggcgc ctgaccagcc ggatgagcga
     4081 ggcgcaggaa cgcgcggtga ttgccgcatc gcgcgacgcc gtggcgcagg tggtgggcga
     4141 agcaccgcgg ggatggtgca gccaggactt cactgaatca gcgaacaccc cggcgctgct
     4201 ggcggaagcc gggttcgcct acaccaccga ctgggcgaac gacgaccggc cctatctgct
     4261 gggaccctat ggaggccagc acctggtggc gctgccgccg caaccggagt ggaacgacct
     4321 cgaatgcatg tggctgcgcc gcatcacgcc accggtgtgg gcggacggca tcaccgaggc
     4381 cttcagcttt ctgcatgagg agggaggcgg cgcgttcaac ctgacgctgc acccgtggat
     4441 cgccggccag gcgcaccgca tccgctggct gcgtgaagca ctgtcccgcg tgctcggcaa
     4501 gccggcggtc tggcgcacca caacggacga actg
//
LOCUS       DASSZH010000147         2998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1367082, whole genome shotgun sequence.
ACCESSION   DASSZH010000147 DASSZH010000000
VERSION     DASSZH010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367082"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..1371)
                     /locus_tag="VFN42_03130"
     CDS             complement(133..1371)
                     /locus_tag="VFN42_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995637.1"
                     /translation="MILAYEACWPGTVHVTVNSAILQIMARAFPTQSIRVLADPSHLR
                     ELQRDPFLASSPALSFAPAGLSRHYRYRTNIVSLRRFLHEFRTLAAALRRMPAGEPLL
                     IVLLSATPTAIFAASALARLRGGGCRVQVVLHGNLNDLQGWRPRNPVLRSLDLKTALE
                     AGHGGRVRFLVLEAGIHRALARLSPRAAAQTDVLPHPVSPVEAAIVAPPPASPPLRVG
                     FVGMATPDKGIDLFLDIARDMRARHGDRVAFYLVGSAREGTDPASFAPLAHPVDFGLL
                     SREDFTARLAMLHYVMLPFQAGYYTLSASGGLIDAITWLKPILATRTPTVEDMFAGYG
                     DIGVLCDDADGLRMALETTLAEGIGDRYAGRVGAMHRLRDSRMPETLSRQYHALTRAH
                     FPAMPDHRDSAFRVLRPTDP"
     gene            complement(1368..2891)
                     /locus_tag="VFN42_03135"
     CDS             complement(1368..2891)
                     /locus_tag="VFN42_03135"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET8995638.1"
                     /translation="MASVGSHVPHGALMGQFVRGFGWIFAWRMTTRVLGMASTLLLVR
                     LLAPADFGLVALSSSFLQAIDAFSALGVEEAVVRTHDPDRRLYDTAFTMIVLRSAGMA
                     LLMALGAAPLARFFGDSRLTPVVLVTAAVTLLSGFENVGTLEFRRDMRFDREFKLQVV
                     PRLVGVVTAIVAALIWRDYWALVIGTAAGRVIRLPSSYLMHPFRPRLTLAAWRQLIGF
                     SFWTWLLCMVRLVRDRIHVFVLGRVLGTAALGTYSIALEIATLPGGELVLPMGRALFS
                     AISVARRSGGDPEAIWLRVVGLAALVIFPAGVGMALVAEPLVRLMLGAQWMMAVPLVQ
                     IVAASAAFMMFDHFCHIQLDATGLVQIDFRGNCITAAVRVALALALVPKFGMAGAIWG
                     LAAAGLLDQIVYLAIKKSVLPFQPLLLLHQVWRPAIATGGMALAVTWSGLGRLPPGAD
                     WSAAILHLLAPALLGAVVYAGVLLLAWQLAGRPAGAEADILAGLRQRLARVQPSMCA"
BASE COUNT          542 a         1053 c         1031 g          372 t
ORIGIN      
        1 cgggtttccg cgacggattg ctgcaggcgg cgcggacggc caacgaaggc tccgtccgcc
       61 cggcatcgct caccctgccg cgcgactggg gcgaggcgct cgacccggtc gcgcgcaggg
      121 tggcggccat cgtcagggat ccgtcgggcg caggaccctg aacgcgctgt cccgatggtc
      181 cggcatggcg gggaaatgcg cccgcgtgag ggcgtggtac tgccgggaca gggtttcggg
      241 catgcggctg tcgcgcagcc tgtgcatggc gcccacgcgg ccggcgtagc ggtccccgat
      301 cccctcggcc agtgtcgtct ccagcgccat gcgcagaccg tcggcatcgt cgcacagcac
      361 gccgatgtcg ccgtatccgg cgaacatgtc ctcgaccgtc ggcgtgcgtg tcgccaggat
      421 gggcttgagc caggtgatgg cgtcgatcag cccgccgctt gccgacagcg tgtagtatcc
      481 cgcctggaac ggcagcatga catagtgcag catggcgaga cgcgcggtga agtcctcgcg
      541 tgacagcagc ccgaaatcga ccggatgggc cagtggcgca aaggacgcgg ggtcggtgcc
      601 ctcccgcgcg ctgccgacga gatagaacgc cacgcgatcc ccgtgccgcg ccctcatgtc
      661 gcgcgcgatg tccaggaaga ggtcgatgcc cttgtccggc gtggccatgc cgacaaagcc
      721 gacacgcaac ggtggcgagg cgggcggcgg cgccacgatg gccgcctcga ccgggctgac
      781 gggatgcggc agcacatcgg tctgcgcggc cgcccgtggc gacagccgcg ccagggcgcg
      841 gtggatgccg gcttccagca ccaggaagcg cacccgcccg ccatgtccgg cttccagggc
      901 ggtcttcaga tccaggctgc gcagcaccgg attgcgtggc cgccagccct gcaggtcatt
      961 cagattgcca tgcagcacca cctgcacgcg gcagccgccc ccacgcagcc gtgccagggc
     1021 ggaggcggcg aagatcgccg tgggcgtggc cgacagcagc acgatcagca acggctcccc
     1081 ggccggcatc cgccgcagcg ctgcggcgag ggtccggaac tcgtgcagga agcggcgcag
     1141 cgagacgatg ttggtccgat agcggtaatg ccgtgacagc ccggcggggg caaagctcag
     1201 ggcgggactg cttgccagga acggatcgcg ctgcagctcg cgcagatgcg aaggatcggc
     1261 gagcacccgg atcgactggg tgggaaaggc gcgcgccatg atctgcagga tggcgctgtt
     1321 gacggtcacg tgcactgtac ccggccaaca tgcctcgtag gccaggatca tgcgcacatg
     1381 ctgggctgca cgcgcgccag acgctgccgc aggccggcga ggatatcggc ctcggcgcca
     1441 gccgggcgcc ccgcgagctg ccatgccagc agcagcacgc cggcatagac cacggcaccg
     1501 agcagcgccg gggccagcag atgcaggatc gccgcgctcc agtccgcacc aggcggcagc
     1561 cgccccagac ccgaccatgt cacggccagc gccattccgc cggttgcgat ggccgggcgc
     1621 cagacctgat gcagcagcag cagcggctgg aacggcagca cggatttctt gatcgccaga
     1681 tagacgatct ggtccagcaa cccggccgcg gccagtcccc agattgctcc ggccatgccg
     1741 aatttcggca caagtgcgag tgccagcgcc acgcgcaccg ccgcggtgat gcagttgccg
     1801 cggaagtcga tctgcaccag tcccgtggca tcaagctgga tgtggcagaa atggtcgaac
     1861 atcatgaacg cggcggacgc ggcgacgatc tgcaccaggg gaaccgccat catccattgc
     1921 gctccgagca tcagccgcac cagcggttcg gcgaccagcg ccatcccgac cccggcgggg
     1981 aagatgacga gcgcggcaag tccgaccacg cgcagccaga tcgcctccgg gtcgccgcct
     2041 gaccgtcgtg ccacggaaat ggccgaaaac agcgcccggc ccatcggcag gacgagttcg
     2101 ccaccgggca gggtggcgat ctccagcgcg atcgaatagg tgcccagggc cgccgtgccg
     2161 agaacccggc ccagcacgaa gacatggatg cggtcccgca ccagccggac catgcacagc
     2221 agccaggtcc agaacgaaaa gccgatcagc tggcgccatg ccgcgagcgt caggcgcgga
     2281 cggaacggat gcatcaggta gctcgacggc aggcgtatca cccgtcccgc cgcggtcccg
     2341 atcacgaggg cccagtagtc gcgccagatc agcgccgcca cgatcgccgt cacgacaccg
     2401 accagccgcg gcaccacctg caacttgaac tcgcggtcga accgcatgtc acggcggaat
     2461 tcgagggtgc cgacgttctc gaagccgctg agcagcgtca ccgcagccgt caccagcact
     2521 accggcgtca gccgggagtc gccgaagaac cgcgcgagcg gcgcggcccc cagcgccatc
     2581 agcagcgcca tcccggccga gcgcagtacg atcatggtga aggcggtatc atacagccgg
     2641 cgatcggggt cgtgggtgcg caccaccgcc tcctcgacgc cgagcgccga gaatgcgtcg
     2701 atcgcctgca ggaacgagga cgagagcgcc accagcccga aatccgccgg cgccagcagg
     2761 cggaccagca gcagcgtgct ggccatgccg aggacccgcg tggtcatgcg ccaggcaaaa
     2821 atccagccga agccgcgcac gaactggccc atcaacgcgc catgcgggac gtgcgagccg
     2881 accgacgcca tgcggggtgg aaccttgtca ggggggatgc gcgccactct catgtccccc
     2941 ctctagtgga gtccccggcc cggatgcaat gaaacattcc gggttccggt accaactc
//
LOCUS       DASSZH010000148         4540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_753343, whole genome shotgun sequence.
ACCESSION   DASSZH010000148 DASSZH010000000
VERSION     DASSZH010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753343"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VFN42_03140"
     CDS             complement(<1..260)
                     /locus_tag="VFN42_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995639.1"
                     /translation="MTPEAARNVFWSGAEAACSGALSFLSACVVARMVGPAELGLGAA
                     AVAMHVLLWVVVNALFADALVQQGATDPATVSAALWAAVAVG"
     gene            complement(257..1645)
                     /locus_tag="VFN42_03145"
     CDS             complement(257..1645)
                     /locus_tag="VFN42_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995640.1"
                     /translation="MRQRVTSATGVPSDGMPRVAAHRAAAVLLGSALLLWPALYNGYP
                     LVFADTGTYLSQAIERYLGWDRPVFYSLFLLPLHLTLTTWPVIAAQALLTGYTLHLLQ
                     QVLLPGSRALWLVPLLAGASLATALPWFAAQLMPDVFTPLLVLALAMLVCTPARLTAW
                     QRRWLMACAACLIAAQHSSVPLAFLLLLALPPLRVRLAGGDAPNRRDWLRLAAPPLLA
                     VLLLVAVNLAGQRRLAIAPYGNVFLLARVIYDGPGLHALQQDCPNSGWRLCAWRDRLP
                     PTSDDFLWRPDSPAIRIGGHKALSAEADAIIASALRREPGAEARAILHNLRRQLGSFA
                     TGDGLHAWPDAVGRWMARDFPPREQAAYATARQSRNRLVVPGWMQSLHAATGLAGVAG
                     CVILLAAGLYRPHPAAGFAAAVLLALLFNAVITGGLSAPHDRYQSRVMLLPPLVTVLG
                     GAALLRDRWSVA"
     gene            complement(1713..2522)
                     /locus_tag="VFN42_03150"
     CDS             complement(1713..2522)
                     /locus_tag="VFN42_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HET8995641.1"
                     /translation="MNHLLRDLAPISAAAWQQIEEEAGRTLKTTLAARKLVDFSGPRG
                     WDTAAVGLGRSEPVPTPAGSEAEARVRRVLPLVELRVAFDLRRAELDAAERGAKDIDL
                     APAIQAARRIAIAEDRAVFHGHTAAGIRGMCETGGGAALGLGGEVTGYPKLIATAIGR
                     LRDRGIGGPYAVALSERAYTELTEATQHGYPVLQHVRRLIGDGPLVWAPGLDGALVVS
                     MRGGDFELTVGQDFSIGYLSHTAETVRLYLEETFTFWNITPEAAEPLLPEA"
     gene            complement(2526..2885)
                     /locus_tag="VFN42_03155"
     CDS             complement(2526..2885)
                     /locus_tag="VFN42_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="encapsulin-associated ferritin-like protein"
                     /protein_id="HET8995642.1"
                     /translation="MAGESLHEDRNVLSQETLDRHRAISTIMEELEAIDWYDQRIEAT
                     GNPELAQILTHNREEEKEHASMLLEWLRRHDPGFDRQLRAQLFREGPIRETEAAGGGD
                     AAASGDGSLGIGSLRGR"
     gene            complement(2974..3315)
                     /locus_tag="VFN42_03160"
     CDS             complement(2974..3315)
                     /locus_tag="VFN42_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HET8995643.1"
                     /translation="MPDTISYADFERVDIRVGTIIEATAFPEARKPAFKLVIDFGPEI
                     GTRRSSAQLTVHYRPDQLIGRQVCAVVNFPPRQIGPFMSEVLTLGMPDEDGAVVLIRP
                     DFKVPNGGRLF"
     gene            3425..>4540
                     /locus_tag="VFN42_03165"
     CDS             3425..>4540
                     /locus_tag="VFN42_03165"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET8995644.1"
                     /translation="MRDYHLPGRSPVYATHGMAATSMPAATLTALDVLRAGGNALDAA
                     VAACAVQCVIEPQSTGIGGDCFCLYVPAGSGKPIALNGSGRAPAAATIDWYERQGMTA
                     IENTSAHAVSVPGAISAWETLLAAHGRKGLDELLQPAIRFAAEGFPVSPKVSWDWERN
                     EAKLRKTGALPFLPGGSAPKPGDRFVQPALAETLRGIARHGARAFYEGAVAADIVATL
                     RAHGGLHTEEDFAAALGGADFVEPITLHWRGYDVWQIPPNGQGIIGLMILGMLAKLGT
                     APDGPLGVTRIHRHVEAARLAYRDRDAFIADMSQVHVPVEKLLSDEYLSSLAGLIRDD
                     RAIAQLPRAGEAVLPPHRDTVYLCVVDKDGNACSFINS"
BASE COUNT          706 a         1617 c         1594 g          623 t
ORIGIN      
        1 cagccgacgg cgaccgcggc ccacagcgcg gccgacaccg tggccggatc ggtcgcgccc
       61 tgctgcacca gggcatcggc aaacaacgcg ttcaccacca cccacagcag gacgtgcatg
      121 gccaccgcgg ccgcgccgag gcccagctcc gccggcccca ccatgcgcgc caccacacag
      181 gcggacagga aggacaatgc accggaacag gcggcctcgg cgccggacca gaagacgttc
      241 ctggcggcct cgggcgtcac gccacgctcc accggtcgcg cagcagcgcg gcgccgccga
      301 gtactgtcac cagcggcggc aacagcatga cgcggctctg gtagcggtcg tgcggcgccg
      361 acaggccgcc ggtgatcacg gcgttgaaca gcagcgccag tagcaccgcg gcggcaaaac
      421 cggcggcggg gtgcgggcgg tacaggccgg ccgccagcag gatgacgcat ccggcaacgc
      481 cggccagccc ggttgccgcg tgcagcgatt gcatccagcc gggcaccacc aggcggttcc
      541 ggctttgccg ggcggtggcg taggcggcct gctcccgggg cgggaagtcg cgcgccatcc
      601 agcggccgac cgcgtccggc caggcatgca gcccgtcgcc ggtggcgaat gaccccaatt
      661 gccgccgcag attgtgcagg atcgcgcgcg cctccgcgcc gggttcgcgg cgcagtgcgc
      721 tggcgatgat cgcgtcggcc tcggcggaca gcgccttgtg cccgccgatc cgtatcgccg
      781 gactgtccgg ccgccagagg aagtcgtcgg aggtcggcgg cagccggtcg cgccaggcgc
      841 aaagccgcca gccggaattc gggcagtcct gttgcagcgc gtgcaggcca gggccgtcgt
      901 agatgacccg cgcgagcagg aacacgttgc cgtacggtgc gatcgccagg cggcgctgcc
      961 cggccagatt caccgcgacc agcagcagca ccgcgagcag gggcggcgca gccaggcgca
     1021 gccaatcccg ccggttcgga gcatcgccgc cggcaagccg cacgcgcaat ggcggcaagg
     1081 cgagcagcag cagaaaggcc agcggcacgc tggaatgctg cgcggcgatc aagcaggcgg
     1141 cgcatgccat cagccagcgc cgctgccagg cggtcagacg cgccggcgtg cagaccagca
     1201 tggccagtgc cagcaccagc agcggggtga acacgtccgg catcaactgc gccgcaaacc
     1261 acggcagggc ggtagccagg cttgccccag ccagcaacgg caccagccac agcgcccggc
     1321 tgccgggcag cagcacctgc tgcagcagat gcagcgtata gccggtcagc agcgcctgcg
     1381 cggcgatcac cggccaggtc gtcagcgtca ggtgcagcgg cagcaggaac aggctgtaga
     1441 agaccggccg gtcccagccg aggtagcgct cgatcgcctg cgacaggtag gtgccggtat
     1501 cggcgaagac gagcgggtag ccgttgtaca gcgccggcca gagcagcagc gccgaaccca
     1561 gcagcaccgc ggccgcgcga tgggccgcga cgcgcggcat gccgtcggac ggaacgccag
     1621 tcgcggaggt gactcgctgc cgcattccgg cgttatgcaa ggcgccggtt gaaggaagcg
     1681 tttacccggc acgccgctgt gtgtggcggc gcctaggcct ccggcaacag cggttccgcc
     1741 gcttccgggg tgatgttcca gaaggtgaag gtctcctcca ggtacagccg cacggtttcc
     1801 gccgtgtgcg acaggtagcc gatggagaaa tcctgcccga cggtcagctc gaaatcgccg
     1861 ccgcgcatcg acacgaccag cgcgccatcc agcccgggcg cccagaccag cggcccgtcg
     1921 ccgatcagcc ggcggacatg ctgcagcacc gggtaaccgt gctgggtggc ttcggtcagc
     1981 tcggtatagg cccgttcgct cagcgcgacg gcataggggc cgccgatgcc gcggtcgcgc
     2041 agccggccaa tggcggtggc gatcagcttg ggatagccgg tcacctcgcc gcccaacccc
     2101 agcgcggcgc cgccgccggt ctcgcacatg ccgcgaattc cggccgctgt atggccatgg
     2161 aacaccgcgc ggtcctcggc gatggcgatg cgccgcgccg cctggatcgc cggcgcgagg
     2221 tcgatgtcct tcgccccccg ttccgccgcg tccagttcgg ctcggcgcag gtcgaaggcc
     2281 acgcgcagtt ccaccagcgg cagcacgcgg cgcacccgcg cttccgcctc actgccggcg
     2341 ggggtgggga ccggttcgct gcggccgagc cccacggcgg cggtgtccca gccgcgcggg
     2401 ccggagaagt ccacgagctt gcgggcggcg agcgtggttt tcagcgtgcg gccggcttct
     2461 tcctcgatct gctgccaggc ggcagccgaa atcggggcaa ggtcacgaag cagatgattc
     2521 atggttcaac gcccccgcag gctgccgatg cccaggctgc cgtcgcccga tgcggcggca
     2581 tccccgccac cggccgcttc ggtttcccgg atcggcccct cgcggaacag ttgcgcgcga
     2641 agctgccggt cgaagcccgg atcgtgccgg cgcagccatt ccagcagcat ggaggcgtgc
     2701 tccttctcct cctcgcggtt gtgcgtgagg atctgcgcca gttcggggtt gccggtggcc
     2761 tcgattcgct ggtcgtacca gtcgatcgcc tccagttcct ccatgatggt ggaaatggcg
     2821 cggtgccgat ccagcgtttc ctggctcaga acgttgcggt cttcgtggag cgattcaccc
     2881 gccatgccgg ttccctctcg tgtcgcagcc gagaggttgg tccaggcggg tggcaggcaa
     2941 ccgtcctggc ggtcagcact gcgttatcgc cggctaaaac agccggcccc cgttcggcac
     3001 cttgaagtcc ggccggatca gcacgaccgc gccgtcctcg tccggcatgc ccagggtgag
     3061 cacctcgctc atgaagggac cgatctggcg tggcgggaaa ttcaccaccg cacagacctg
     3121 gcggccgatc agctggtccg gccggtagtg caccgtcaac tgggcggagg accgcctggt
     3181 gccgatctcc gggccgaaat cgatcaccag cttgaacgcc ggcttgcgcg cttccgggaa
     3241 ggcggtggcc tcgatgatgg tgccgacgcg gatgtccaca cgctcgaaat ccgcgtagct
     3301 gatcgtgtcc ggcatggctg ccccctctgt ggcggcggat catgccacgc cgcgtcgcat
     3361 tgccaaacgg ggacatgccc ggcagtttgc cccggaaaat ccgtcctgcc aggaatcttg
     3421 cccgatgcgc gactaccacc tccccggccg cagcccggtc tatgccacgc acggcatggc
     3481 cgcgacctcc atgcccgcgg cgacgctgac cgcgctggac gtgctgcggg ccggcggcaa
     3541 tgcgctcgat gccgctgtgg cggcctgcgc cgtgcagtgc gtgatcgagc cgcaatccac
     3601 cggcatcggc ggcgactgct tctgcctgta tgtgccggcg ggctccggca aaccgatcgc
     3661 gctgaacggc tcgggccgag cgcccgccgc ggcgacgatc gactggtatg agcggcaggg
     3721 gatgacggcg atcgagaaca cctcggcgca tgcggtcagc gtgccgggcg cgatcagcgc
     3781 gtgggagacg ctgctggcgg cgcatggccg caaggggctc gacgagctgc tgcagccggc
     3841 gatccgcttc gccgcggaag gctttccggt gtcgccgaaa gtgtcctggg actgggagcg
     3901 gaatgaggcc aagctgcgca agaccggtgc gctgcccttc ctgcccggtg gatcggcccc
     3961 caaaccgggc gaccgcttcg tgcagcccgc gctggccgag acgctgcgcg gcatcgccag
     4021 acacggcgcg cgcgcgttct atgagggcgc ggtggcggcc gacatcgtcg ccacgctgcg
     4081 tgcccatggc gggctgcaca ccgaagagga tttcgccgcc gcgctgggcg gcgcggattt
     4141 cgtcgaaccg atcaccctgc actggcgcgg ctatgacgtg tggcagatcc cgccgaatgg
     4201 ccagggcatc atcggcctga tgatcctcgg catgctggcg aaactgggca ccgcgcccga
     4261 cggtccgctc ggcgtcaccc gcatccatcg ccacgtggag gccgcgcgcc tggcctatcg
     4321 cgaccgcgac gccttcatcg ccgacatgtc gcaggtgcac gtgccggtgg agaagctgct
     4381 gagcgacgag tacctctcca gcctggccgg gctgatccgc gacgaccgcg ccatcgcgca
     4441 actgccccgc gccggcgagg cggtgctgcc gccgcatcgc gacacggtct atctctgcgt
     4501 ggtggacaag gacggcaacg cctgcagctt catcaactcg
//
LOCUS       DASSZH010000149         4670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_941167, whole genome shotgun sequence.
ACCESSION   DASSZH010000149 DASSZH010000000
VERSION     DASSZH010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941167"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VFN42_03170"
     CDS             <1..571
                     /locus_tag="VFN42_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HET8995645.1"
                     /translation="FSPELEAACMDRIIQPALAEMARLGTPFRGILFAGLMLTEEGPK
                     LIEFNVRFGDPECQVLLLRLRSDLLPALQAACDGELANFDLRWHDKAAIAVVLAARGY
                     PDAPERGSQIRGLERASAITGVEVFHAGTQEDSAGHLHAAGGRVLTVCALAPSLREAS
                     DAAYAAVRAIDWPEGFCRRDIGWRALPPG"
     gene            complement(586..1410)
                     /locus_tag="VFN42_03175"
     CDS             complement(586..1410)
                     /locus_tag="VFN42_03175"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET8995646.1"
                     /translation="MLLSRRMLLRHGLVLAGLTSSAALPGHSALAAPRRPAWHRPPRP
                     LVVLDPGHGGKDPGCIGHHGTHEKHVSLAIGLELRRQLLAHRVCRVAMTRASDVFIPL
                     EQRVAIAERHKAALFVSIHENSCPSRLVHGASVYTFATHASDPDSARLAARENRADRF
                     GGPKFKHYSPQVSRILRSLLSRETRIHSAELQHDLVHRLDHRVGLVQRPARHAHFVVL
                     EATNIPSVLIETGFLSNPHEEKLLRTRHHQIVVATSIRSAISRYLVEDAKTLRLTR"
     gene            1864..3156
                     /locus_tag="VFN42_03180"
     CDS             1864..3156
                     /locus_tag="VFN42_03180"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET8995647.1"
                     /translation="MTHPLSVAIAGLGTVGGGLLKLLRDNADLVAARAGRPIAVTAVS
                     ARDRTKDRGVSLAGLRWYEDPVALAADPAVDVVVELIGGADGPARTLVEAALARGKPV
                     VTANKAMLAVHGAELAAAAEERNIPLAFEAAVAGGIPVIKAVREGLAGNRIDRIAGIL
                     NGTCNYILTQMRERGREFAEVLADAQKLGYAEADPSFDIDGVDAAHKLAILAALAFGR
                     PVAFDAVHVEGIRRISSLDIAFATELGYRIKLLGIARQVDDGISARVHPCMVPANAPI
                     ARVDGVFNAVVAEGDFVGRVMLEGRGAGAGPTASAVVADLIDIARNRHTPMWGAAAAD
                     LSNAPSVPMSAHVGAYYLRLMVVDRPGVIADVTAVLRDLGVSLESMLQRGRAPGEAVP
                     VVLVTHETQESAMRAALERIAGLDAVLEQPALIRIEAG"
     gene            complement(3190..>4670)
                     /locus_tag="VFN42_03185"
     CDS             complement(3190..>4670)
                     /locus_tag="VFN42_03185"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HET8995648.1"
                     /translation="AAPTLPRRPAVRRSLVLAACLMATFMAAVESTIVATAMPTIVAD
                     LGGFGLFSWVFAVYLLTQAIAIPIYGRMADIYGRRKVFYVGVGVFLTGSTLCGFAPSM
                     VWLVLFRALQGFGAGGVQPIAMTICGDIYTPSERAHVQGLISSVFGVSAVVGPTLGAF
                     LVEHVAWPVVFWVNLPVGAAAIVMISLFLREDVAPRQHRIDLLGSVLMMLGIGAVMLL
                     LVQGGTLVRWAMLSAGVVALGSFAWLFLHESRTAEPMLPLELWRDRVIAISSLGSGLA
                     GAVMMGISAFLPTYVQGAMQRSPTIAGLVLGAMSVTWAFSSILGGRLMVRTTYRLAAA
                     LGAGALILGSMALLLMRPSEGLFWAVTGSLVIGIGMGLCNTAFIVSIQAAVPWAKRGA
                     ATSSCLFLRFIGQALGASGFGAVLNLSIEHAAPGAASQVDRLLEPALRATLSGPQRAQ
                     LVNLVAGGVHNAYWLALALAIAALLLVLQLPARLSPSQQTLR"
BASE COUNT          841 a         1521 c         1587 g          721 t
ORIGIN      
        1 gttctcgccg gagctggaag cggcctgtat ggaccgcatc atccagcctg ccttggcgga
       61 aatggcacgg ctcggtacgc cgtttcgcgg catcctattc gccgggctga tgctgaccga
      121 ggaaggtccg aagctgatcg aattcaacgt ccgcttcggc gaccccgaat gccaggtgct
      181 gttgctacgg ctgcgctccg atctgctgcc ggcgttgcag gcggcctgcg acggcgaact
      241 ggcgaatttc gacctgcgct ggcacgacaa ggcagcgatc gctgtggtgc tggcagcacg
      301 cggctatccg gacgcacccg aacgcggcag ccaaatccgc ggtctggaga gggcgagcgc
      361 gatcaccggg gtggaggtgt tccacgccgg cacccaagag gacagtgccg ggcatctcca
      421 cgccgcgggc gggcgcgtgc tgacggtctg cgccctggcg ccatccttgc gggaagcgtc
      481 ggacgccgcc tatgccgcgg tgcgggcgat cgactggccc gagggatttt gccgccgcga
      541 tatcggctgg cgggctttgc cccccggcta attagcgacc ttaattcagc gagtcaggcg
      601 cagcgtcttc gcatcctcca ccaggtagcg gctgatcgcc gatcgtatgg acgtcgccac
      661 gacgatctgg tggtgccgcg tgcgcagcag cttttcctcg tgcggattcg acaggaagcc
      721 ggtctcgatc agcacgcttg gaatattcgt ggcctccagc acgacaaaat gcgcatgccg
      781 tgccggacgc tggaccaggc cgacgcgatg atccaggcgg tgtacaagat cgtgctgcag
      841 ttcggcggag tggatacggg tttcgcggct gagcagactg cgcaggatgc gggaaacctg
      901 cggggaataa tgcttgaact tcgggccgcc aaaccggtcg gcacgatttt cccgcgccgc
      961 cagcctggcg ctgtccggat cggacgcatg cgtggcgaag gtatagacgc tggcgccatg
     1021 cacgaggcgg gacggacagc tgttctcgtg gatcgacacg aacagcgcgg ccttgtggcg
     1081 ttcggcgatg gcgacgcgct gctccagcgg aatgaatacg tccgaggcac gggtcatcgc
     1141 cacgcgacag actcgatgcg ccagcaattg gcgacgcagt tccaggccga tggcgaggga
     1201 cacatgcttc tcatgcgtgc cgtgatgtcc gatgcagccg ggatccttgc cgccatggcc
     1261 tggatccagc accaccaacg gccgcggtgg ccgatgccag gcgggccttc gcggcgctgc
     1321 cagcgcgctg tgtcctggca aagcggcgga cgatgtcagg ccggccagaa cgagaccatg
     1381 gcgaagcagc attcggcgcg acagcaacat gccgcctgcc taagtccaga aaataaggac
     1441 gtcaactaag ttattgatat tgtaatctca gagtcagatt tcggcttgct tgatcgctgg
     1501 caaaaatcaa tgccggcatg gcgcagaaca gtattggcat tgcacgattt cgtccgcaac
     1561 ggcacatcat catagggtat cagatgaata tattgattgg ttccaacgca caccaaaaat
     1621 accaatgcgg tttcataaaa caaaaacaga gttcgggcgg gttgcattcc caaggcggca
     1681 atgaaataca caccagaact gagggaattt tgggcgcctt gccgggttgg cagccatgga
     1741 gccggccagt gaacgcgggc cggttgggca tccgcccccg gtcactgcta tggtccggtg
     1801 ctccacccag accaagcaac aggattccac catgatgccc gtcccgcacg ccggcctgac
     1861 gccatgaccc accccctctc ggttgccatc gctggcctgg gcaccgtcgg cggtggcttg
     1921 ctgaagctgc tgcgcgacaa cgccgacctg gtcgccgccc gtgccggccg cccgatcgcc
     1981 gtcaccgccg tctccgcccg tgaccgtacc aaggaccgcg gggtctcgct tgccgggctg
     2041 cgctggtacg aagacccggt ggcgctggcg gcggatccgg ctgtggacgt agtggtggag
     2101 ctgatcggcg gcgccgacgg tccggcccgc accctggtgg aggctgcgct ggcccgcggc
     2161 aagccggtgg ttaccgccaa caaggcgatg cttgcggtgc acggcgcaga attggccgcc
     2221 gcggccgaag aacgcaacat ccccctcgcc ttcgaagcag ccgtcgcggg tggcatcccg
     2281 gtgatcaagg cggtgcgcga gggtctggcg ggcaaccgga tcgaccggat cgccggcatc
     2341 ctgaacggca cctgcaacta tatcctgacg caaatgcgcg agcgcggccg tgaattcgcc
     2401 gaggttctcg ccgacgccca gaagctcggc tacgccgaag ccgacccgag cttcgatatc
     2461 gatggcgtgg acgcggcgca caagctggcc atcctcgccg cgctggcctt cgggcgcccg
     2521 gtggcgttcg atgcggtgca cgtcgaggga atccgccgca tttcctcgct cgacatcgcc
     2581 ttcgccactg agcttggcta ccgcatcaag ctgctgggga tcgcccgcca ggtggatgac
     2641 ggcatcagcg cccgggtgca cccctgcatg gtgccggcga atgcgccgat cgcccgggtg
     2701 gacggtgtgt tcaacgcggt ggtggccgag ggcgatttcg ttgggcgcgt catgctggaa
     2761 ggccgtggtg ccggcgccgg cccgactgcc tccgccgtcg tggccgacct gatcgacatc
     2821 gcccgcaacc ggcacacccc catgtgggga gcggccgcgg cggatttgtc gaacgcgcct
     2881 tccgtgccga tgagcgcgca tgtcggcgcg tattatctcc gcctgatggt ggtggaccgg
     2941 ccgggagtga tcgcggatgt caccgccgtg ctgcgcgacc tcggcgtatc gctggaatcc
     3001 atgctgcagc gcggccgagc gcccggggaa gcggtacccg tggtgctggt gacacacgag
     3061 acgcaggaaa gcgcgatgcg tgcagcgctg gagcggattg ccggattgga cgcagtgctg
     3121 gagcagccag cgctgatccg aatcgaagcc ggctgagccg ggccgcgcgc aagccgctgt
     3181 gtttcgtgtt cagcgcagcg tctgctgcga cgggctcagc cgtgccggca gctgcaacac
     3241 caggagcagt gcggcgatcg cgagcgccaa cgccagccag taggcgttgt gcacgccgcc
     3301 tgccacgaga ttgactaatt gcgcccgctg cgggcccgac agcgtcgccc gcaaagctgg
     3361 ttccagcagt cgatccacct gcgaggcggc gccgggcgcg gcgtgctcga tgctcaggtt
     3421 cagcaccgcg ccaaatccgc tcgcgcccaa ggcctggccg atgaagcgca ggaacaggca
     3481 ggacgacgtc gcggcgccgc gtttggccca gggcaccgcc gcctggatgg atacgatgaa
     3541 ggcggtgttg cacaggccca tgccgatgcc gatgaccaac gagccggtca ccgcccagaa
     3601 cagcccttcc gagggccgca tgagcagcag cgccatgctg ccgaggatca gggctcccgc
     3661 ccccagcgcc gctgccagcc ggtaggtcgt gcgcaccatc agccggccgc cgaggatgct
     3721 ggagaatgcc caggtgaccg acatggcgcc cagcaccagg ccggcgatgg tcggcgaccg
     3781 ctgcatggcg ccctgcacgt aggtcggcag gaaggcggat atgcccatca tcaccgcgcc
     3841 ggccagtccg ctgcccaggc tgctgatcgc gatcacccgg tcgcgccaga gttccagtgg
     3901 cagcatcggt tcggcggtgc gcgattcgtg caggaacagc caggcgaagc tgcccaatgc
     3961 cacgacaccg gcgctgagca ttgcccagcg caccagggtg ccgccctgca ccagcagcag
     4021 catcaccgcg ccgatgccga gcatcatcag caccgaacca agcaggtcga tgcggtgctg
     4081 gcggggtgcc acgtcctccc gcaggaacag cgaaatcatc acgatcgccg cggcgccgac
     4141 cggcaggttc acccagaaca ccaccggcca ggcgacatgc tccaccagga aggcgccaag
     4201 tgtgggaccg acaacggcgg atacgccgaa gaccgaggag atcagcccct ggacgtgcgc
     4261 gcgttccgac ggtgtgtaga tatcgccgca gatcgtcatc gcgatcggct gcacgccgcc
     4321 ggcaccgaaa ccttgcaggg cacggaacag caccagccag accatgctgg gggcgaagcc
     4381 gcacagcgtg gatccggtga ggaatacgcc gacgccgaca tagaacactt tgcgccggcc
     4441 atagatgtcc gccatgcggc cgtagatcgg aatggcgatc gcctgggtca gcaggtagac
     4501 ggcgaacacc cagctgaaca ggccgaagcc gcccaggtcg gcgacgatgg tcggcatggc
     4561 ggtggcgacg atggtgctct ccaccgccgc catgaaagtg gccatcaggc aggcggccag
     4621 caccagggaa cggcggacgg cagggcggcg cggcagggtg ggggcggcat
//
LOCUS       DASSZH010000150         4869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_514834, whole genome shotgun sequence.
ACCESSION   DASSZH010000150 DASSZH010000000
VERSION     DASSZH010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514834"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VFN42_03190"
     CDS             complement(<1..395)
                     /locus_tag="VFN42_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995649.1"
                     /translation="MKAAHTNLPGLPPPPDPPQHRYTQPLAPANAELLLLWQVLLVYA
                     RHLLGLLRQAGERGLALLARGFGSMQASFVLTNLTRGILRAIALERVLLARAAGTQAG
                     GSQAGGQPDPAASNARKRTPRAPRQAATEA"
     gene            694..1272
                     /locus_tag="VFN42_03195"
     CDS             694..1272
                     /locus_tag="VFN42_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET8995650.1"
                     /translation="MKIPAVMDVAEFLAWDAPAGQRWQLLDGAPQAMAPASRTHNVIQ
                     GELGRLIANHLVEQGRRCSLVPNAGIVPRIRSDSNLRIPDLAVTCSGYQEEEYVVSDP
                     VLLIEILSPSNRAETWANVWAYATLPSVQEILVLHSTRIRAELLRRNAAGEWPDRPAV
                     IENGMLELHSIGFNIPLTAAYRGTRLDREAAS"
     gene            1350..2081
                     /locus_tag="VFN42_03200"
     CDS             1350..2081
                     /locus_tag="VFN42_03200"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET8995651.1"
                     /translation="MGRLKGKIILISGGARGQGAAEARLFVAEGARVVIGDVLENEGR
                     ALAAQLGESTVFVRQDVTSETDWAAAVQAAEKLGGLHGLVNNAGIYQPRRLMETDAEL
                     FERHMRINQLGCFLGMKAVVPAMERSGGGSIVNISSVAGLRGSPGAIAYSATKWALRG
                     MTKAAAIDLAPKQIRVNSVHPGPIDTEMLKVRTPEQNQQRLQAVPMRRMGTAEEVANL
                     VLFLLSDESGYITGAEVAIDGGATL"
     gene            2109..3524
                     /locus_tag="VFN42_03205"
     CDS             2109..3524
                     /locus_tag="VFN42_03205"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET8995652.1"
                     /translation="MPRDALKSILPVAGLSAALADQVEFTGGADPVLPTPFRIGMAGA
                     ATLAATGLAAAHLWEMRCGRRQNVAVDLRQATASLRSGTYMQLGDGDVSHERNTIMGV
                     YPTKDGRWSYLHCNFPNHRAAALSVLGCAEDRAAVAQAVKGWNALELEEAIIAAKGAG
                     GMVRTKAEWAQHPQAAAIAALPLLEIVRIGDSPPEELPKGSRPLSGIRVLDLTRVLAG
                     PTCARTLAEHGADVMKITAAHLPNLGYQELDTGHGKLSAQLDLREPANVDTLRGLVRE
                     ADVFSQGYRPGTLGSRGLSPEELAAIRPGLVYVSLCAFSHAGPWASRRGFDTVVQTVS
                     GITARQAEVVPGKSPGPQFYPVSAIDYCTGYLMAFGAMVALARRAQEGGSWLVRISLA
                     QVGKWIVDLGEVPEAAARDTPAEFTADELQRWSMVTETPAGRLRHLKPVVQLSETPPH
                     WARPSVPLGYHPPVWPPRGTA"
     gene            3532..3801
                     /locus_tag="VFN42_03210"
     CDS             3532..3801
                     /locus_tag="VFN42_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995653.1"
                     /translation="MAEEDAAKPRFTARQGQYLAFIHAYTLVIGQPPAEADMQRFFRV
                     SAPSVHQMVVTLHESGLISRQSGASRSIRVLVDRSDLPQLEPVEK"
     gene            complement(3999..4304)
                     /locus_tag="VFN42_03215"
     CDS             complement(3999..4304)
                     /locus_tag="VFN42_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HET8995654.1"
                     /translation="MARLRHRGATAKAARLAAWQEEVVLFHADRIFPVDVAVAVRAGI
                     LLAGAEAAGFHPGAEDAWIAATAELNGLALLTFNEADFGAMRVPCRNPVKDLPPDRG"
     gene            complement(4455..4706)
                     /locus_tag="VFN42_03220"
     CDS             complement(4455..4706)
                     /locus_tag="VFN42_03220"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HET8995655.1"
                     /translation="MRSWPLQEARAHLRDVIDGALESGPQRITRYGKQAVIVISESEW
                     ERRSAVRRSFGELLANSPLSASDLPPRLPLRASRDNGFE"
BASE COUNT          813 a         1594 c         1724 g          738 t
ORIGIN      
        1 gcctcggtgg cggcctgtcg cggcgcgcgc ggcgtgcgct tgcgggcgtt gcttgcggcc
       61 gggtcgggct ggccgccggc ttgggagcct ccggcttggg tgcctgcggc gcgcgccagc
      121 agcacgcgtt ccagcgcgat ggcgcgcagg atgccgcggg tgaggttggt gagaacgaag
      181 ctggcctgca tcgagccgaa gccgcgcgcg agcaacgcca ggccgcgctc acccgcttgc
      241 ctgagcaagc cgagcagatg gcgggcatag accagcagga cctgccagag gagcagcagt
      301 tcggcgttcg ccggcgcaag cggctgggtg tatcggtgct ggggtggatc gggagggggc
      361 ggcaggccgg ggaggttggt gtgtgctgct ttcatgacat gtcatataca ctaactcact
      421 gactgttgtc aatggcacag ttgcatggcg tcaccccggc gtgcatccag cgccgacctg
      481 cccatggtga tcgcctggcg gcatgccgcg gccgttcgcc cgtgacgctg cttcatcggc
      541 ttcgtagcgt tccaccagag cccgcgcgaa ccggctggcc cacgcgggtg gtattggcgc
      601 cctgcaaagc cagagggctg ccgccgcggc gtggcagatg gggctggggt gcagcgccgc
      661 cgacgtgcta tactgcccgc catggaaccg gcagtgaaaa ttccggctgt catggacgtc
      721 gcggagttcc tggcatggga cgcaccggcc gggcagcgct ggcagttgct ggacggcgcc
      781 ccgcaggcca tggcgcccgc cagccgcacg cataacgtca tccagggaga actcgggcgc
      841 ctgatcgcca atcatctggt cgagcagggc cgccgctgca gcctggtgcc gaatgccggc
      901 atcgttccgc gcatccgttc cgacagcaac ctgcgcatcc ccgatctggc ggtcacctgc
      961 tccggctatc aggaggagga atatgtcgtc agcgatccgg tgctgctgat cgagatcctc
     1021 tctcccagca accgcgccga gacctgggcg aatgtctggg cctacgccac gctgcccagc
     1081 gtgcaggaaa tcctggtgct gcacagcacg cggatccgtg ccgaactgct gcggcgcaat
     1141 gcggcgggcg aatggcccga ccggccggcc gtgatcgaga acggcatgct ggaactgcac
     1201 agcattgggt tcaacatccc gctcacggca gcttatcgcg gcacccggct ggatcgcgaa
     1261 gcggcgtcct gactgtcgct gccagacggt cggttgtgat ccaggcccta ggcgcccatc
     1321 attcccgcct gtttcgcggg gaggaagcga tggggcgcct gaagggaaaa atcatcctga
     1381 tcagcggcgg cgcgcgcgga cagggtgccg ccgaagcgcg gctgttcgtt gcggaaggcg
     1441 cccgcgtggt gattggcgac gtgctggaaa acgaaggccg cgccctcgcg gcgcagcttg
     1501 gcgagtccac ggtgttcgtg cggcaggacg tcaccagcga aaccgactgg gccgcggcgg
     1561 tgcaggcggc ggagaaactc ggtggcctgc atgggctggt caacaatgcc ggcatctacc
     1621 agccgcgcag gctgatggag acggatgcag agctgttcga gcgccatatg cgcatcaacc
     1681 agcttggctg cttcctcggc atgaaggcgg tagtgccggc gatggagcgg tcgggcggcg
     1741 gatcgatcgt caacatctcc tcggttgcgg gcctgcgcgg ttcgcccggc gccattgcat
     1801 acagcgccac caaatgggca ctgcgcggca tgaccaaggc cgccgcgatc gacctcgcgc
     1861 cgaagcagat ccgggtgaac tcggtgcatc ccggcccgat cgacacggag atgctgaagg
     1921 tgcgcacgcc ggagcagaac cagcagcggc tgcaagcggt gccgatgcgc cgcatgggca
     1981 ccgccgagga ggtggcgaac ctggtgctgt tcctgctgtc ggacgaaagt ggttacatca
     2041 ccggcgccga ggtcgcgatc gacggcggcg ccacgctgta gtcaaccggg agccgaggag
     2101 aaaacgcgat gccgcgagat gcgctgaagt cgatcctgcc tgttgccggc ctgtccgccg
     2161 ctctcgccga tcaggtggaa ttcaccggcg gcgccgatcc cgtgctgccg acgccgttcc
     2221 gcatcggcat ggcgggagcc gccacgctcg ccgccaccgg cctggccgcg gcgcatctgt
     2281 gggaaatgcg ctgcggacgc cgacagaacg tcgcggtcga tctgcgccag gccaccgcat
     2341 cgctgcgcag cggcacctac atgcagctgg gcgacggcga tgtttcgcac gaacgcaaca
     2401 ccatcatggg cgtgtatccg accaaggatg ggcgctggag ctacctgcac tgcaacttcc
     2461 ccaatcaccg cgccgccgcg ctgagcgtgc tgggctgcgc cgaggatcgc gcggcggtgg
     2521 cgcaggcggt gaaaggctgg aacgcgctgg agctggaaga agccatcatc gccgccaagg
     2581 gcgccggcgg catggtgcgc acgaaagcgg agtgggcgca gcatccgcaa gcggcggcga
     2641 tcgccgcgct gccgctgctg gagatcgtgc gcatcggcga cagtccgccg gaagagctgc
     2701 cgaagggcag ccggccgctg tccggcatcc gcgtgctgga cctgacgcgc gtgctcgccg
     2761 gcccgacctg cgcccgcacg ctggcggagc acggcgccga cgtcatgaag atcaccgcgg
     2821 cgcatctgcc gaacctcggc tatcaggagc tggataccgg gcacggcaaa ctgtcggccc
     2881 agctcgatct gcgcgagccg gccaatgtgg acacgctgcg cgggctggta cgcgaggcgg
     2941 acgtgttctc gcagggctat cgtcccggca cgctgggcag ccgcggcctg tcgccggagg
     3001 aactggcggc gatccgcccg ggactggtgt acgtgtcgct ctgcgcgttc agccatgccg
     3061 gcccgtgggc ctcgcggcgc ggcttcgaca ccgtcgtgca gaccgtcagt ggcatcaccg
     3121 cgcggcaggc cgaggtggtg ccgggcaagt cgccgggccc gcaattctat ccggtctcgg
     3181 cgatcgacta ctgcaccggt tacctgatgg cgttcggcgc catggtcgca ctggcacggc
     3241 gcgcacagga aggcggcagt tggctggtgc ggatttcgct ggcacaggtc ggcaagtgga
     3301 tcgtcgatct gggcgaagtg ccggaggccg cggcgcgcga taccccggcc gagttcaccg
     3361 ccgacgaact gcaacgctgg tcgatggtga cggaaacgcc ggccgggcgg ctgcggcatc
     3421 tgaaaccggt ggtgcaactg tcggagacac cgccgcactg ggcgcgcccg tcggttcccc
     3481 tgggctacca tccaccggta tggccgccac gcggaacggc ttgaggcagg catggctgaa
     3541 gaggacgccg cgaagccgcg gttcaccgca cgacagggac aatatctcgc gttcatccac
     3601 gcctatacgc tggtgatcgg ccagccgccg gcagaagccg acatgcagcg gttcttccgg
     3661 gtcagtgcgc cttcggtgca ccagatggtg gtgacattgc atgagagcgg attgatcagc
     3721 cggcaatcag gcgcgtcgcg cagcattcgc gtgctggtcg atcgcagcga cctgccgcag
     3781 ctggaaccgg tcgagaaata gcgcgaacgc ccatgccgct gtcgtcgctg tccggacaag
     3841 gggacacggg gccgctgccg tggtggccgg ctgcggcgcg cgctgaacgg caacgctggc
     3901 gacaaggacg tgcagcggca gcacgatgag ccgcacgatc tcacgggctc ccgcgttcgc
     3961 cgcaccggct cgggctggac caggcgctgc ggccccgatc agccgcgatc gggtggcaaa
     4021 tccttcacgg gattccggca aggcacacgc atcgcgccga agtcagcctc attgaaggtg
     4081 agcagtgcca atccgttcag ttcggctgtc gcggcgatcc aggcatcttc ggcaccgggg
     4141 tggaatccgg cagcttcagc gcccgcgagc aaaatcccgg cgcggaccgc gaccgcgacg
     4201 tccaccggga agatccggtc tgcgtgaaac agcaccactt cctcttgcca tgcggcaaga
     4261 cgagccgcct tggctgtcgc accacgatgg cgcaaccgcg ccacgccata ggcgactcag
     4321 tgagcgagac cgcgctgagg tagcactcat ctccgttccg ccgcagccat ccggccaaat
     4381 tcgtgtcggg ggcggacttg tcgggactca ggtcggacag aacgcacgtg tcgagaaggt
     4441 acaacgcaga gcgctcattc aaacccgttg tcgcggctcg cgcgtagtgg cagtcgcggc
     4501 ggcaggtcgg acgcgctcag cggcgaattg gccagcagct ctccgaatga ccgccggacg
     4561 gcggagcggc gctcccattc cgattcagag atgacaataa ccgcctgctt gccgtagcgg
     4621 gtaatgcgct gcgggccgga ttccagcgca ccgtcgatta catcgcgcag atgggcgcga
     4681 gcctcctgta agggccagct acgcacggcc aactcctgta cattttgtat aggacttagg
     4741 gcgccaggga gagtgccgca agtctcacac gccgccccgc cggccccgga taccgatcgc
     4801 ttgccgccag tagcgggcag ttcggagagc ggcagaggca cagggccggt ctgagcgagg
     4861 cacgcccct
//
LOCUS       DASSZH010000151         2228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1588791, whole genome shotgun sequence.
ACCESSION   DASSZH010000151 DASSZH010000000
VERSION     DASSZH010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1588791"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="VFN42_03225"
     CDS             <1..1057
                     /locus_tag="VFN42_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8995656.1"
                     /translation="PAGFTGFAPHTPMSMAGMMRGMSMSGPMTMTAPPWLQGAGGLRS
                     LQAAVRLSDGMWLSFATALPNAAPPTQWPFFAATATMAVIVVLASAWAVGRVTAPLRL
                     LAQAAERLGRDVNAPPILEAGTREMRQATQSFNQMQARIRRLVDNRTRMLAALSHDLR
                     TPLTLLRLRTEGLPESEDRDRMLATIGELDTMIGASLSFARDQAVAEAKRRTDVGALL
                     TSIVDDMADAGLPVTVASAESVVLDCQPVSLRRALTNLIDNAVKYGGSAHVAIAATAS
                     SVTITIDDSGPGIPEAELQKVFEPFHRLEESRSRETGGTGLGLSIAQSIVQAHGGDIV
                     LANRPEGGLRASIFLPR"
     gene            complement(1199..2005)
                     /locus_tag="VFN42_03230"
     CDS             complement(1199..2005)
                     /locus_tag="VFN42_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995657.1"
                     /translation="MFAFIRNLVGVKTDQAVNGAIEAIVRWDPQSATEAELRTMEQHL
                     DDLGRQVAQARMSFDKEKREADAIQALSHQRMAAAEQLNGQIESATDPNRKAELERSL
                     ASLLGMLEQMAPDIEREKQDAEDAKSFLESLEQTYQQAGRKLKSARDELNRAQRDMGR
                     AAQQREMAERRAEAARQAAGLSGATSGLSVALKAMQDNATRDLAAAEAAAAKAKLLQP
                     TRPEKDDPNIAAAMAQVSGGAPAQLSLSDRLQALRQRQAAPRQISGPARS"
     gene            complement(2039..>2228)
                     /locus_tag="VFN42_03235"
     CDS             complement(2039..>2228)
                     /locus_tag="VFN42_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HET8995658.1"
                     /translation="VTLDIVLWGLVALAIQLLTFVLAAALICDLRSMIERGNVAAAWL
                     LVGIQLAVALLNAGAMAG"
BASE COUNT          320 a          751 c          753 g          404 t
ORIGIN      
        1 gccggccggc ttcaccggct tcgctcccca cacgccgatg agcatggccg gcatgatgcg
       61 cggcatgtcg atgtcgggcc cgatgaccat gacagcgccc ccctggctgc aaggcgccgg
      121 cgggctgcgg tcgttgcagg ccgcggtgcg gctgtccgat ggcatgtggc ttagcttcgc
      181 cacggcgctg ccgaacgccg cgccaccgac gcagtggccg ttctttgccg cgacggcgac
      241 gatggcagtg atcgtcgtgc tcgcctcggc ctgggcggtc ggccgcgtca ccgcgccgct
      301 gcgccttctg gcgcaagccg ccgagcggct cgggcgtgac gtgaacgcgc cgccgattct
      361 ggaggcggga accagggaga tgcgccaggc gacgcagtcg ttcaaccaga tgcaggcgcg
      421 catccgccgg ctggtggata accgcacccg catgctggcg gcgttgtcgc acgacctgcg
      481 cacgccactg acgctgctgc ggctgcggac cgagggattg ccggagagcg aggaccgcga
      541 tcgcatgctg gccaccatcg gcgaactcga tacgatgatc ggcgcgtcgc ttagcttcgc
      601 ccgtgatcag gcggtggccg aggccaagcg gcgcaccgat gtcggcgcgt tgctcacctc
      661 catcgtggac gacatggcgg atgccggact gccggtaacc gtggcttccg ctgaatcggt
      721 ggtgctggac tgtcagccgg tgtcgttgcg acgggcgctg accaacctga tcgacaatgc
      781 ggtgaagtac ggcggttcag cgcatgtggc gatcgccgcg acagcatcca gcgtgaccat
      841 cacgatcgac gacagcggcc cagggattcc tgaagcagag ctacagaaag tgttcgagcc
      901 gttccatcgg ctggaggaat cgcgcagccg ggaaaccggt ggcaccgggc ttggtctatc
      961 gatcgcgcag tcgatcgtgc aagcgcatgg cggcgacatc gtgctggcga accgcccgga
     1021 aggtgggctg cgtgccagta tctttctgcc acgctgatcc gcaatcccgc gctttgaggg
     1081 atcgcgtgcg cagattggcc ctgcaattgc gcacctcgtc gtggcacgct ccgcccgcca
     1141 cgactgatat gcgttctggt cgccactctg aggacgcagc ataagctcgt atgctgtatc
     1201 agcttcgtgc cggtccgctg atctgccgtg gcgcggcctg ccgctggcgc agcgcctgca
     1261 accgatccga caggctgagc tgtgccggcg cgccgccgga aacctgcgcc atggccgcgg
     1321 cgatgttcgg gtcgtccttt tccggccgcg tcggttgcaa caacttggcc ttcgccgccg
     1381 ccgcttccgc cgcggcaagg tctcgggtgg cattgtcctg catcgccttc agcgcgacgc
     1441 tcaacccgct ggtggcgccg gacaggccgg ctgcctgacg cgctgcctcg gcgcggcgtt
     1501 ccgccatctc gcgctgctgc gcggcgcgac ccatgtcgcg ctgggcgcgg ttcagctcgt
     1561 cgcgcgccga tttcagcttg cggccggcct gctggtaggt ttgctccagg ctttccagga
     1621 agctcttggc gtcttcggca tcctgtttct cgcgctcgat gtcgggcgcc atctgctcca
     1681 gcatgccgag cagactggcg aggctgcgtt ccagttccgc cttgcggttc gggtcggttg
     1741 cgctttcgat ctgaccgttc agctgctccg ccgccgccat gcgctggtgc gacagcgcct
     1801 ggatggcatc cgcctcgcgc ttttccttgt cgaacgacat gcgcgcctgc gccacctggc
     1861 ggccgaggtc gtccaggtgc tgttccatcg tgcgcagctc cgcttcggtg gcggattgcg
     1921 ggtcccagcg gacgatagcc tcaatggcgc cgttgaccgc ctggtcggtt ttcaccccga
     1981 cgagattgcg gatgaacgcg aacatgtcat ggctccttct gggtgaattg gatgcagact
     2041 agccggccat cgcgccggca ttgagcaaag ccaccgcgag ctgaatgccc accagcagcc
     2101 aggcggcggc gacgttgcct cgttcgatca tgctgcgcag gtcgcagatc agcgccgcgg
     2161 caagcacgaa ggtcagcagc tggatggcaa gcgcgaccag cccccacagc acgatgtcga
     2221 gcgtcacc
//
LOCUS       DASSZH010000152        10611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1554770, whole genome shotgun sequence.
ACCESSION   DASSZH010000152 DASSZH010000000
VERSION     DASSZH010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 10611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10611
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554770"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VFN42_03240"
     CDS             complement(<1..405)
                     /locus_tag="VFN42_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET8995659.1"
                     /translation="MRAMLLDAPHRNLRLVAAAIPSPGPGQLQIRVHACAVCRTDLHV
                     VDGELTEPKLPLVPGHEIIGTVSALGSGVDRFAIGDRVGVPWLGWTCGACAFCRSGRE
                     NLCPQARFTGYQIDGGYAEYAVADQRFCFAIPD"
     gene            664..2556
                     /locus_tag="VFN42_03245"
     CDS             664..2556
                     /locus_tag="VFN42_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011253119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HET8995660.1"
                     /translation="MSTAAAAAETDRKPPLTAAALLAVLGIVYGDIGTSPLYAFKASL
                     QIFTGLPIADTEILGVLSLLFWSLILIVTVKYVTLVMRADNRGEGGILALMALAQRVS
                     GSERTRNIVALIGIAGACLFFGDGLITPAISVMSAVEGLEVSAPQLQEYVLPISATII
                     VLLFAVQWRGTSKVGRVFGPVTAVWFIALAVLGLVQIVQHPYVLYALSPTWAVALCIK
                     YKGLAFIVLGAVVLVVTGAEALYADMGHFGARPIRVTWTFFVLPSLVLNYFGQGALMI
                     SHPDALSNPFFLLAPHWLRLPLVVLATAATVIASQAVISGAYSVTRQCMQLGFLPRMT
                     VRHTSNTEEGQIYVPQVNTMLAVGVLVLVFAFKTSDNLASAYGIAVTGTFLCTAVLAA
                     IVFRRQFHWSRTAAMTVFGLFFLIDLVFFSANTLKVVEGGWVPLAFGLALTGMMTSWK
                     RGRELQLARWKQDSMPLAPFLARLPQSRTIRVPGMAIFLTGNPDYVPTSLLHNLKHNK
                     VLHERVLFVNVHTADEPEVPASDRAHVSELAAGIYRVVLRYGFMESPNIPRALEELNG
                     EIKFDPMQASYFVGREVLVPAMVPKMPWWRLWLFLAMARNAVPATEFFRIPSDRVVEL
                     GVRVAI"
     gene            complement(2580..3275)
                     /locus_tag="VFN42_03250"
     CDS             complement(2580..3275)
                     /locus_tag="VFN42_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HET8995661.1"
                     /translation="MRIAEMDWRMVEDWVRHDDRCILPIGSTEQHAGLSLATDAIVAE
                     RVAAEAAEPLGIPVFPVLPFGLTPYFSSFPGTITLRVATYAALLRDVLDSLKQTGFRR
                     VLVVNGHGGNQPAAAVAREWMMDNPDARVRFHDWWQAPLTAAAIRKIDPVAGHASWME
                     NFPWTRLPNREWVEGEKPMVDVEQLRALPPFEVRLMLDDGNFGGRYQRDDGEMLEIWD
                     VAVRETRALLEEW"
     gene            complement(3343..4140)
                     /locus_tag="VFN42_03255"
     CDS             complement(3343..4140)
                     /locus_tag="VFN42_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET8995662.1"
                     /translation="MSAPDSMLVFDRAAVRRHRDRAAAAVDSVADLLRDAADRLLDRL
                     DDTTIRFSRALDVGGRSCIAPLLRARGIDTVSCDLSAAMAARNPGPAVAADEEFLPFA
                     PRSFDLVVASLSLHWINDLPGALIQLRHALRPDGLLLASIPVLGTLTELRQALTEAEA
                     TLRGGASPRISPFPDLRDCASLLQRAGFAMPVADLEEIRMLYANPLALLSDLRAAGET
                     NALRLRDRGIPPRALFAAALTALPEQDGRVPVTVRLAMLTGWAPPES"
     gene            complement(4365..5429)
                     /locus_tag="VFN42_03260"
     CDS             complement(4365..5429)
                     /locus_tag="VFN42_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HET8995663.1"
                     /translation="MRAWAVVENGKPLQELEWPTPEPKGTEVLLEVTHCGVCHSDLHI
                     WDGYYDIGGGKKMSLTDRGVKLPLAMGHEIVGRVVKLGPQASGVKVGDLRIVYPWLGC
                     GECDACRSDNDNMCTVVPRSLGVYANGGYGTHVIAPHPRHLVDPGNIDPAVAATYACS
                     GITVYSAIKKVMPIAPDEAIVLVGAGGLGLNAIAILKAMGHRNIIVVDISAEKREAAL
                     KAGASKAVDGNGDGATQRIIEAAGGPVLAVVDLVNGTQTARLAFNALRKGGKLIQVGL
                     FGGELTVPLPIMAIRALTVRGSYVGSVTDLRELVELAQKGQLEALPVATVPQNQANDA
                     LMRLKDGKVTGRLVLKAEAA"
     gene            complement(5558..5631)
                     /locus_tag="VFN42_03265"
     tRNA            complement(5558..5631)
                     /locus_tag="VFN42_03265"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5597..5599),aa:Cys,seq:gca)
     gene            5768..6454
                     /locus_tag="VFN42_03270"
     CDS             5768..6454
                     /locus_tag="VFN42_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HET8995664.1"
                     /translation="MTPADLTSDIDRYADARNNMVDSQLRPNKVTDPRITGAMRRLPR
                     ERFLPPNLRHLAYVDEDVPLGNDRVLMEPMVIARLIQLAAPMAGERALVVAAGVGYGA
                     AVLSACGPRVTALEEDPRLVELARGALSALAPEINVVAGAIAAGWPSEAPYDVILIEG
                     AVHAIPDAIGRQLRVNGGRLVTVLRNNGGRTSSAVLAEATSMGLRPQPMFDCATPDLP
                     SLRPAPVFEF"
     gene            complement(6462..6965)
                     /locus_tag="VFN42_03275"
     CDS             complement(6462..6965)
                     /locus_tag="VFN42_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995665.1"
                     /translation="MPDTPVAPASGEPYDPSIALRLSRLEDEMREERAARSQVEAAII
                     RIEAILPHLATRTDIADLRADIARVETKADLAAGKADLAAERAHLAAEKATLAAEKAN
                     LAAGTSDHLAAKIDGMQLDIVGLRKDVTRLEVALADKPSKLYMWGVLTALLTAYACGL
                     AALAILK"
     gene            7055..8305
                     /locus_tag="VFN42_03280"
     CDS             7055..8305
                     /locus_tag="VFN42_03280"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8995666.1"
                     /translation="MRSLPVMQRRSSTSSRDWTQDAPGTLRDMPLLPLAATLAVQTLA
                     TMAMFSVPAIAPEIARELHVSGTLVGDFVAAAYGTGIISALLSPGLIRRYGGVRATQA
                     VLLAAAGMVLVCAGISNVFGLALGAVVLGLGYGAAAPASTHLLVPQTPKSVFNMVMSL
                     RQIGVPLGGVIGSLVVPPLTVAIGWRGALLTELVPMLLLIGLMEIPRRRWDADRDPGR
                     RAFGPTLLQPFAMLHERAIRRLSVASFVYSGMQLCFVAFMTVQLTTTAGFDLVAAARM
                     LAAYQIAGTVSRPIWGWIADTWLTPRQTLVIHGLGMAVAAVLAGYFSAGWSSWAVLGV
                     VLLAGCTAGGYTGVAYAEYAALGGTRRTEATGLGTALMFAAVMSIPPLFGFAVAAAGG
                     YAVPFLALAGLAVISAAVLLLPQL"
     gene            8302..8820
                     /locus_tag="VFN42_03285"
     CDS             8302..8820
                     /locus_tag="VFN42_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HET8995667.1"
                     /translation="MSGRSRSLSEADRAVWAAYAQQLHALPGRAVPPPPASPAVLPPR
                     PAPLAQVHTARPITRPLAIGDAAGGVDNATWQRFRSGKITPSRKLDLHGMTAQRAFQA
                     LVAFVRSAHAERLRCVEVVTGRGSGETGGVIRREFPLWLNLPDIRPLVLGASYPHAAN
                     PGAVRLLLRRIR"
     gene            8817..9227
                     /locus_tag="VFN42_03290"
     CDS             8817..9227
                     /locus_tag="VFN42_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET8995668.1"
                     /translation="MTPFGARLRALRAERGITLKQLAAALQVSTAYLSALEHGRRGAP
                     SAGLVHQVNEFLGLIWDDAEELARLARLSNPRVTVNTAGLSPEQTALANRLSQTIHRL
                     PPETVAALHAILDTTTPQPKPRLRRAARRERPDS"
     gene            complement(9235..9438)
                     /locus_tag="VFN42_03295"
     CDS             complement(9235..9438)
                     /locus_tag="VFN42_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995669.1"
                     /translation="MQTTALARVLAVHRASQRPAARLDSAAPDAPAKPHPLLLAIARN
                     ARLEPRTLLEMARDRNGVRRPWQ"
     gene            complement(9643..>10611)
                     /gene="ychF"
                     /locus_tag="VFN42_03300"
     CDS             complement(9643..>10611)
                     /gene="ychF"
                     /locus_tag="VFN42_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019086085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HET8995670.1"
                     /translation="GRVAVPDKRLDVLAAIGKSAKIIPTSLEFVDIAGLVRGASKGEG
                     LGNQFLANIREVDAIIHVLRCFEDPDVTHVEGPIDPLRDAEIVETELMLADLESLEKR
                     LNTAQKRARGGDKESIAAVAVMEPLVAALQDGKPARTAIPPGQDEAVRRLQLLTSKPV
                     LYVCNVEEAAAATGNAFSARVAERAKAEGARTVIVSAAIEAEVAQLPEADRAEFLEGL
                     GLHDSGLDRVIRAGYDLLGLITYFTVGPKETRAWTIVQGTKAPQAAAVIHKDFERGFI
                     ACETIAYDDFVAGRGEQGAKEAGKMRVEGKDYAVKDGDILLFRFNV"
BASE COUNT         1551 a         3594 c         3681 g         1785 t
ORIGIN      
        1 gtccggaatg gcgaagcaga agcgctgatc ggcgacggca tactccgcat agccgccgtc
       61 gatctgatag ccggtaaatc gcgcctgggg acacaggttc tcgcgccccg agcggcagaa
      121 cgcacaggcg ccgcaggtcc agcccagcca gggcacgcca acacggtcgc cgatcgcgaa
      181 gcggtccacg ccggagccga gggccgagac ggtgccgatg atctcgtgac cggggaccag
      241 gggcagcttc ggttcggtca gctcgccgtc caccacatgc aggtcggtgc ggcacacggc
      301 gcaggcatgt acccggatct gcagctggcc agggccgggg gaggggatgg cagccgcaac
      361 caaccgcagg ttgcggtgcg gagcatcgag cagcatggca cgcatcggtg gtctcctcgg
      421 gatcatcacg atcggcctgc cggcggggtc aagcccggcg gtcgaggaac cgcggcagcg
      481 tagcacaatt cgcctgggcc tgccgttccg cttgtgtcgt gccgggccgc ggcgtatgcg
      541 tccgcggctc gccggcgggt tgcctggcag cgcgaccgat cacagcgagg gataccgcga
      601 tcgcatgccg ccgacaggcc ccacccggac gacgcgcgcc gcgttcgacg ctcgagaccc
      661 cgcgtgagca cagccgcggc cgccgccgaa actgaccgta agccgccgct gaccgctgcc
      721 gcgctgctgg cggtgctggg catcgtctat ggcgacatcg gcaccagccc gctctatgcc
      781 ttcaaggcca gcctgcagat cttcaccggg ctgccgatcg ccgataccga gatcctcggc
      841 gtgctctccc tgctgttctg gtcgctgatc ctgatcgtca cggtcaaata cgtgacgttg
      901 gtcatgcggg cggacaaccg gggcgaaggt ggaatcctgg ccttgatggc gctcgcgcaa
      961 cgcgtctcgg gcagcgagcg aacccgcaat atcgtggcat tgatcggcat cgcgggcgcc
     1021 tgcctgttct ttggcgacgg gctgatcacg ccggcaatct ccgtcatgtc ggcagtcgaa
     1081 ggcctggaag tctccgcgcc gcagctacag gaatacgtgc tgccgatcag cgcgacgatc
     1141 atcgtgctgc tgttcgccgt gcaatggcgc ggcaccagca aagtcggccg cgtgttcggc
     1201 ccggtgaccg cggtgtggtt catcgcgctc gccgtgctgg ggctggtgca gatcgtgcag
     1261 cacccatacg tgctgtacgc gctatcgccg acctgggcgg tggcgctgtg catcaaatac
     1321 aaaggcctgg ccttcatcgt gctgggcgcc gtggtgctgg tcgtcaccgg cgccgaggcg
     1381 ctgtacgccg acatgggcca tttcggcgct cggccgatcc gcgtgacctg gacgttcttc
     1441 gtgctgccgt cgctggtgct gaactatttc ggccagggcg cgctgatgat cagccacccc
     1501 gacgcgctca gcaatccatt cttcctgctg gcgccgcact ggctgcggct gccgctggtg
     1561 gtgctggcca ccgcggcgac ggtgatcgcc agccaggcgg tgatctccgg cgcctattcg
     1621 gtcacccgcc agtgcatgca gctcggcttc ctgccgcgca tgacggtgcg gcacacctcc
     1681 aatacggagg aaggccagat ctacgtgccg caagtgaaca ccatgctggc ggtgggcgtg
     1741 ctggtcctgg tgttcgcatt caagaccagc gacaatctgg cctccgctta cggcatcgcg
     1801 gtcaccggca cgttcctgtg caccgccgtg ctggccgcca tcgtgttccg ccgccagttc
     1861 cactggtcgc gcaccgcggc catgacggtg ttcggcctgt tcttcctgat cgacctggtg
     1921 ttcttctccg ccaacacgct gaaggtggtg gaaggcggct gggtgccgct ggccttcggc
     1981 ctggcgctga ccgggatgat gacctcgtgg aagcgcggcc gggagctgca actggcgcgc
     2041 tggaagcagg acagcatgcc gctggcgccc ttcctcgccc ggctgccgca atcccgcacc
     2101 atccgcgtgc cgggcatggc gatcttcctc accggcaacc cggattatgt gcccacgtcg
     2161 ctgttgcaca atctgaagca caacaaggtg ctgcatgagc gggtgctgtt cgtgaacgtg
     2221 cacaccgcgg atgaaccgga ggttcccgcc agcgaccggg cgcatgtcag cgagctggcc
     2281 gcgggcatct atcgcgtggt gctgcgttac ggcttcatgg aaagcccgaa catcccgcgc
     2341 gcgctggaag aactgaacgg cgagatcaag ttcgacccga tgcaggccag ctacttcgtc
     2401 ggccgcgagg tgctggtgcc ggcgatggtg cctaagatgc cgtggtggcg gctatggctg
     2461 ttcctggcga tggcgcgcaa cgccgtgccg gcgacggagt tcttccgcat tccgtccgac
     2521 cgcgtggtgg agctgggggt gcgcgtggcg atctgaccgg ccgccggtcg gccgagacgc
     2581 taccattcct ccagcagcgc gcgggtttcg cgcaccgcga catcccagat ttccagcatc
     2641 tccccgtcat cgcgctggta gcggccaccg aaattgccgt cgtccagcat cagccgtact
     2701 tcgaagggcg gcagggcgcg cagttgctcc acgtccacca tcggcttctc gccttccacc
     2761 cattcgcgat tgggcaggcg ggtccagggg aaattctcca tccaactggc atgaccggcg
     2821 accggatcga tcttgcggat cgccgccgcg gtgagcggag cctgccacca gtcgtggaag
     2881 cgcacccgcg catcgggatt gtccatcatc cattcgcgcg ccaccgctgc ggccggctgg
     2941 ttgccgccat ggccattgac gaccagcacc cggcggaagc cggtctgctt caggctgtcg
     3001 agcacgtcgc gcagcagcgc ggcataggtg gcgacgcgca gcgtgatggt accggggaag
     3061 ctgctgaaat acggcgtcag cccgaagggc agtacgggaa acacgggaat gccgagcggt
     3121 tcggcggctt cggcggcgac gcgttccgcg acgatggcgt cggtggcgag cgacaagcca
     3181 gcgtgctgtt cggtgctgcc gatcggcaga atgcagcggt catcatgacg gacccagtct
     3241 tccaccatcc gccagtccat ctcggcaatg cgcatcgttt cccccagcgt ccctggaatg
     3301 ggagacgctg gacactggcg gctgttccgt caaccggtgc ggtcaggatt cgggcggcgc
     3361 ccagccggtg agcatggcca gccgcacggt gacgggcacg cggccatcct gttccggcag
     3421 cgctgtcagc gccgcggcga acagcgcgcg cggtggaatg ccgcggtcgc gcaagcgcag
     3481 cgcgttggtt tctccggcgg cacgcagatc gctcagcagc gccagcggat tcgcatagag
     3541 catgcggatc tcttccaggt cagcgaccgg catggcgaat ccggcacgct gcagcagcga
     3601 cgcgcaatcg cgcagatcgg ggaagggtga gatgcgcggc gatgcgccgc cgcgcagggt
     3661 tgcctcggct tcggtcagcg cctggcggag ttccgtcagt gtgccgagca cggggatact
     3721 ggcgagcagc agcccgtcgg gacgcagcgc gtgacggagc tggatcagcg cgccgggcag
     3781 gtcgttgatc caatgcagcg acaggctggc aaccaccaga tcgaaactgc gcggcgcgaa
     3841 gggcaggaac tcctcgtccg cggcgacggc agggccggga ttgcgcgcgg ccatcgcggc
     3901 ggagagatcg cagctcacgg tgtcgatgcc gcgcgcacgc agcagcggcg caatgcagct
     3961 gcggccgccg acgtccaagg cgcgggaaaa gcggatcgtg gtgtcgtcaa gccgatccag
     4021 cagccggtcg gcggcatccc gcagcaagtc ggcgacgctg tcgacagcgg cggcggcacg
     4081 gtcgcggtga cggcgcaccg cggcgcggtc gaacaccagc atggagtcgg gagcggacat
     4141 gagagggaga ggtagggggg ccgatgccgg gggacaaccg cgggcgcgtc acggcagcat
     4201 cccggaaggg gcagcagatc ggcggtgagt tggggactgc ctgcctgctc ccctcccgca
     4261 aggaacccct tgggggaagg gggcatggca gtgctccccg ggaactcacc ggcgagcccg
     4321 cccccttatc cttctgcgag aaggcaagag ggggcccggt ccggtcaggc tgcctcggcc
     4381 ttcagcacca ggcggccggt caccttgccg tccttcaggc gcatcagcgc gtcattggcc
     4441 tggttctgtg gcacggtggc caccggcagc gcttccaact ggcccttctg cgccagttcg
     4501 accagctcgc gcagatcggt gacgctgccg acatagctgc cgcgcacggt cagcgcgcga
     4561 atcgccatga tcggcagcgg cacggtcagt tcgccgccga acaggccgac ctggatcagc
     4621 ttgccgccct tgcgcagcgc gttgaaggcg aggcgggcgg tctgcgtgcc gttcaccaaa
     4681 tccaccaccg ccagcaccgg gccgccggcc gcctcgatga tgcgctgggt ggcgccgtcg
     4741 ccattgccgt ccactgcctt gctggcaccg gccttcagcg ccgcctcgcg cttctccgcg
     4801 ctgatgtcca ccacgatgat gttgcggtgg cccatcgcct tcaggatggc gatggcattc
     4861 agcccgagcc cgccggcgcc gaccagcacg atcgcctcgt ccggcgcgat cggcatgacc
     4921 ttcttgatcg cagaatacac ggtgatgccg gagcaggcgt aagttgcggc caccgccgga
     4981 tcgatgttgc ccggatcgac caggtggcgc ggatgcggcg cgatgacatg ggtgccgtag
     5041 ccgccattgg catagacgcc caggctgcgc ggcaccacgg tgcacatgtt gtcgttgtcg
     5101 ctgcggcagg cgtcacattc gccgcagccc agccagggat agacgatgcg caggtcaccg
     5161 accttcacgc cgctggcttg cggcccgagc ttgaccacac gcccgacgat ctcgtggccc
     5221 atggcaagcg gcagcttcac gccgcggtcg gtcagcgaca tcttcttgcc gccgccgata
     5281 tcgtaatacc catcccagat atgcaggtcg gagtggcaga cgccgcaatg cgtcacctcc
     5341 agcagcacct ctgtaccttt cggttccggg gtgggccact cgagttcctg cagtggcttg
     5401 ccattctcaa cgacggccca ggcgcgcatg gttcagtctc ctactcacgg gtatgcgcgg
     5461 gtctttactg cggtgcgggg cggaagggaa ttgccggcgg cgcaaggatc gcgtgccatg
     5521 gcggtggctt cggattgcga ttgtgcgcgg aggccgctgg aggtccgggc cggaatcgaa
     5581 ccggcatacg cggatttgca gtccgctgca tcaccactct gccaccggac cggtgcagaa
     5641 gcgccgctat atcggccgcc atccggagcc ggtcaagccc ggtcacggca ggcattccac
     5701 cgtgccttgg cgtttttcgc cgctggcggc tataagccgc ccggcccctg catgtggcga
     5761 gttcgcaatg acccctgccg acctgacatc cgacatcgac cgttacgccg acgcgcgcaa
     5821 caacatggtg gacagccagc tccgcccgaa caaggtgacc gatccgcgca tcaccggcgc
     5881 catgcgccgc ctgccgcggg agcgcttcct gccgcccaac ctgcgccacc tggcctatgt
     5941 cgatgaggac gtgccgctgg gcaacgaccg cgtgctgatg gagccgatgg tcattgcccg
     6001 cctgatccag cttgctgccc ccatggcggg cgagcgtgcc ctggtggtcg ccgccggagt
     6061 cggttacggc gccgcggtgc tgtccgcctg cggaccgcgg gtgacggcgc tggaggaaga
     6121 tccccgcctg gtcgaactgg cacgcggcgc gctcagcgcg ctggcgccgg agatcaacgt
     6181 ggtagccggc gcgattgccg ccggctggcc gtccgaggcg ccctacgatg tcatcctgat
     6241 cgagggcgcg gttcacgcca tcccagacgc gatcggcagg cagttgcggg tgaatggcgg
     6301 ccggctggtc accgtgctgc gcaacaatgg cggacgcacc agttccgcgg tgctggccga
     6361 ggcgacctcg atgggtctgc ggccgcagcc gatgttcgac tgcgccaccc ccgatcttcc
     6421 gtcgctgcgc ccggcgcctg tgtttgagtt ctgagcgccg gctatttcag gatggcgagc
     6481 gctgcgaggc cgcacgcata ggcggtcagc agcgcggtca acacgcccca catgtagagc
     6541 ttgcttggct tgtcggcgag cgcgacctca agccgggtga cgtccttccg aagcccgacg
     6601 atatccagtt gcatgccatc gatcttggcc gcgagatggt cggatgttcc tgccgccaga
     6661 ttggctttct ccgccgccag ggtggctttc tccgctgcca gatgggctct ctccgccgcg
     6721 agatcggctt ttcccgccgc caggtcggcc ttcgtctcaa cccgggcgat atccgcccgg
     6781 aggtcggcga tatccgtccg tgtcgcaagg tgcggcagga ttgcttcaat ccggatgatg
     6841 gcagcctcga cctgcgagcg ggcagccctc tcctcccgca tctcatcctc cagccgggac
     6901 aggcgtagcg cgatggaagg atcgtagggt tcgccggagg cgggtgcaac gggcgtgtca
     6961 ggcatgtctc ggctcctgga gcggccgcag catgctccgg accggttaag attgtcctga
     7021 cgcaagactg gcccggcttg ccatcccgcg catcatgcgc tcccttccgg ttatgcagcg
     7081 gcgcagcagc acgtcatccc gcgactggac ccaggacgcg cccggcacgc tgcgtgacat
     7141 gccgctgctg ccgctggcgg ccacgcttgc cgtgcagacg ctggcgacga tggcgatgtt
     7201 ctcggtgccg gcgatcgcac ccgaaatcgc ccgggaactg catgtcagcg gcacgctggt
     7261 cggcgatttc gtggctgccg cgtacggcac gggcatcata tcggccctgc tgtcgcccgg
     7321 gctgatccgc cgctacggcg gcgtgcgcgc gacccaggcg gtgctgctgg ctgccgccgg
     7381 catggtgctg gtctgcgccg gcatcagcaa tgtgttcggc ctcgcgctgg gcgccgtggt
     7441 gctggggctg ggttacggtg ccgcggcgcc ggcgagcacg catctgctgg tgccgcaaac
     7501 gccgaaatcc gtgttcaaca tggtgatgtc gctgcggcag atcggcgtgc cgctgggcgg
     7561 cgtgatcggc tccctggtcg tcccgccgct gacggtggcg atcggctggc gcggtgccct
     7621 gttgaccgag ctggtgccga tgctgctgct gatcgggctg atggagattc cccgccggcg
     7681 ctgggacgcc gaccgcgacc cgggacggcg cgccttcggc ccgacgctgc tgcaaccgtt
     7741 cgcgatgctg cacgaacggg cgatccgccg gctgtcggtc gccagcttcg tctattccgg
     7801 catgcagctc tgcttcgtcg ccttcatgac ggtgcagctg accaccaccg ccgggttcga
     7861 cctggtcgcc gcggcccgca tgctggccgc ctatcagatt gccggcaccg tgagccggcc
     7921 gatctggggc tggattgccg atacctggct gacgccccgc cagaccttgg tgatccacgg
     7981 gcttggaatg gcggtggcgg cggtactggc cgggtatttc tccgccggct ggtcgtcctg
     8041 ggcggtgctg ggtgtggttt tgctcgccgg ctgtacggcg ggcggctaca ccggcgtggc
     8101 ctatgcggag tatgccgcgc tgggcggcac gcgccgcacc gaggccaccg gattgggaac
     8161 cgccctgatg ttcgccgccg tgatgtcgat tccgccgctg ttcggtttcg ccgtcgccgc
     8221 ggccggcggc tatgccgtgc cgttcctggc cctggcgggg ctggcggtca tcagcgcggc
     8281 ggtcctgctg ttgccgcagc tatgagcggg cgcagccgct cgctgagtga ggccgaccgg
     8341 gcggtatggg ccgcctatgc ccaacaactg catgcactgc ccggccgggc agtgcccccg
     8401 ccgccagcca gcccggcggt gctgccgccg cgcccggcgc cgcttgccca ggtgcatacc
     8461 gcgcggccga tcacccggcc gctggcgatc ggcgatgcgg cgggcggggt ggacaacgcc
     8521 acctggcaac ggtttcgctc aggcaagatc accccctccc gtaagctgga cctgcacggc
     8581 atgacggcgc agcgcgcgtt ccaggcactg gtggccttcg tacgctcggc gcatgccgag
     8641 cggctgcgct gcgtggaggt ggtgaccggg cggggcagcg gcgagaccgg cggggtgatc
     8701 cggcgcgagt tcccgctttg gctgaacctg ccggatatcc gcccgctggt gctgggggcg
     8761 tcgtatccgc atgcggcgaa ccccggggcg gtgcggctgt tgctgcggcg gatccggtga
     8821 cgccgtttgg ggcccgcctg cgcgcgttgc gcgcggagcg ggggatcacg ctgaagcagc
     8881 tggcggcggc gttgcaggtg tccaccgcct atctgtccgc gctggaacat ggccggcggg
     8941 gcgcgcccag tgccgggctg gtgcaccagg tcaacgagtt cctcgggctg atctgggacg
     9001 atgccgagga gcttgcgcgg ctggctcgct tgtccaatcc gcgggtgacg gtgaacaccg
     9061 cggggctgag cccggagcag acggcgctgg ccaaccggct gtcgcagacg atccatcgcc
     9121 tgccgccgga aaccgtcgcg gcgctgcacg cgatcctgga caccaccacg ccgcaaccga
     9181 agccgcggtt gcgccgtgcc gctcggcggg agcggccgga ctcctaagcc gcggtcactg
     9241 ccaggggcgg cggacgccgt tgcggtcgcg cgccatctcc agcagagtcc ggggctccag
     9301 gcgcgcgttg cgtgcaatgg cgagcagcaa cggatggggc ttggccgggg cgtcgggcgc
     9361 cgcgctgtcc aggcgtgccg ccgggcgctg cgatgcccgg tgcaccgcca gaacccgcgc
     9421 gagagctgtg gtctgcatac tgcgaactcc gtaccgtgtt ggggggccaa cgcagggcgg
     9481 aagaacacgg ttgcgacaca gcaacgcgta gttgaacggg gcgccaggtg tttgacaggg
     9541 ccgcgagcgc acggccaatc aggtgcgagt ggcttgtggt ctcgcgtatt ccgcagcagg
     9601 tcgtgaatgg cgggccttcg cccaccacgg cgatctcgcc ggctacacgt tgaagcggaa
     9661 cagcaggatg tcgccgtctt tcaccgcata gtccttgcct tcgacgcgca tcttgccggc
     9721 ttccttggcg ccctgctcgc cccgtccggc cacgaaatcg tcataggcga tggtttcaca
     9781 tgcaatgaag ccgcgctcga aatccttgtg gatcaccgca gcggcctgcg gggccttggt
     9841 gccctgcacg atggtccagg cgcgggtttc cttcggccct acggtgaaat aggtgatgag
     9901 gccgagcaga tcgtagccgg cgcggatcac gcgatcgaga ccgctgtcgt gcaggccgag
     9961 accttccagg aactcggcgc gatcggcttc gggcagttgc gccacctccg cctcgatcgc
    10021 cgccgacaca atcactgtgc gggcaccttc cgccttggct cgttccgcca cccgggcgga
    10081 gaacgcgttg ccggttgcgg cggcggcttc ctccacgttg cacacgtaca gcaccggctt
    10141 ggacgtcagc agttgcagcc gccgcaccgc ttcgtcctgt cccggcggaa tcgcggtgcg
    10201 cgccggcttg ccgtcctgca gcgcggccac cagcggttcc atcaccgcca ccgcggcgat
    10261 gctttccttg tcgccgccgc gcgcgcgctt ctgcgcggtg ttcagccgct tctccaggct
    10321 ttccaggtcg gccagcatga gctcggtttc aacgatctcc gcgtcgcgca gcgggtcgat
    10381 cgggccttcc acatgcgtga cgtcgggatc ctcgaagcag cgcagcacgt ggataatcgc
    10441 atccacttcg cggatgttgg cgaggaactg gttgcccagg ccttcaccct tgctcgcgcc
    10501 acgcaccagc ccggcgatgt cgacgaattc cagcgaggtg gggatgatct tcgccgactt
    10561 gccgatcgcc gccagcacgt ccagccgctt gtccggcacg gcaacgcggc c
//
LOCUS       DASSZH010000153         4459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1503744, whole genome shotgun sequence.
ACCESSION   DASSZH010000153 DASSZH010000000
VERSION     DASSZH010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1503744"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..1224
                     /locus_tag="VFN42_03305"
     CDS             226..1224
                     /locus_tag="VFN42_03305"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8995671.1"
                     /translation="MRVSAGAKLLLAGGALLGAIALANRYARPRVTPPPARRRSVQVD
                     GVRLFYLEQGEGTPIVLLHGNGAMAEDFAASGILDRLAVRHRVIAFDRPGFGESERPS
                     TRIWTFPAQADLLRRALVQMGIARPVLVGHSAGCLAALHMALHDPQDTAALVLLSGFY
                     FPRLRPDMLITSLWALPGLRWLAASAAAHLFGWLALPGYLRVVFAPNRVPERFRRSFP
                     LAAVLRPRHLMATIADTPLLLWDPMTLPRCLDRLRMPVTIMTGTQDRIVMGRLQSRRL
                     HQAVPGSRLHALEGIGHMIHHAEPDAVIAAIEQAADAAQRRLGADGIAPENKPVFS"
     gene            1258..2802
                     /locus_tag="VFN42_03310"
     CDS             1258..2802
                     /locus_tag="VFN42_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HET8995672.1"
                     /translation="MDPVVETASGRIRGTVIEGVAAFKSIPYGASTVGANRFLPPRPP
                     QPWAGIRDALDYVGQSPQSRTGMPQRAELATFSGPPDTSPETEDCLTLHVWTPSADPA
                     ARRPVMVWLHGGAFSFGSANSLRLQGMRLCRRGDVVLVTVNQRLNIFGFLDLSQFAGP
                     EYAQSGNAGTLDMLAALHWVRDNIAAFGGDPGNVTIFGESGGGGKVSTLMAMPAARGL
                     FHRAIVQSGAAVRLRTVERAAKLTDAVLHELGLGRAAVAELQAVPMQRLQAAIEPAVK
                     ALGPSPWPLLDRYPFGPVVDGTLLPRQPFDPDATPVSADIPLIIGDTTHEAASFIAYD
                     NRVWSGTLTEDELRRWVAGIAGSHTDRVIALYRQLHPAASPAQRLIATLTDCNFRLRS
                     LLLAERKVKQAGAPVWMYSFAWRTPLFDGRLGAPHALDVPFTFDTLDFTNATDRSAGA
                     HALAGTMATAWANFARSGVPGAEGMPEWPTFRLEDRATMVLDAPCRVARDPGREGRLL
                     WQEIAG"
     gene            2899..3951
                     /gene="queG"
                     /locus_tag="VFN42_03315"
     CDS             2899..3951
                     /gene="queG"
                     /locus_tag="VFN42_03315"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HET8995673.1"
                     /translation="MDHQQTRTDPRPAIRQRALALGFDAIGFAAAELGAEARERLGAF
                     LAAGQHGDMGWLADRAAQRSHPQALWPEARSVIALGLSYAPEDDPLAALDRPDRGAIS
                     VYARNRDYHDVLKGMLKHLAQFIASRFAAGVKVFVDTAPVMEKPLAARAGLGWQGKHT
                     NLVSRQHGSWLFLGEIYTTLVLQPDTPHADRCGSCTRCLSACPTDAFPAAYRLDATRC
                     ISYLTIEHHGPIPHELRPLMGNRIYGCDDCLAVCPWNRFARGSPHDKLRAREALSAPR
                     LAELALLDDAGFRTMFSGSPIKRIGRHRFIRNVLIAIGNSGDPGLLPVAARLRGDADP
                     VVAEAAEWACTQLRRS"
     gene            complement(3994..>4459)
                     /locus_tag="VFN42_03320"
     CDS             complement(3994..>4459)
                     /locus_tag="VFN42_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995674.1"
                     /translation="PAIALADFRAVPARTSRPPADEQSLRVGDWLGMTPLTVTPDYQV
                     QDLADTRETAPLYAAEGIVHPGTILRCCNWALSHNVVLPAWMHMGSTVQNFGVAHVGD
                     SLGVRARVTKNYEHKGHKWVEIDALVLANESTPLARVTHIAIYRPRQLAAAA"
BASE COUNT          625 a         1489 c         1596 g          749 t
ORIGIN      
        1 tgggcctgcc gcccggcgtg cactcctacg ccattctgcc gatcggctat ccgatgggga
       61 atttcggccc ggtcggccgg cgtgatctgt cggaggtggt gtaccaggat cgctggggcg
      121 agccctacac tgcgctctga tccggcagcc ggccggcgcg gtggaacccc tgcgccggcc
      181 accggttgtc gccgggacac ctgactcaag ggccggatga cagtgttgcg cgtttccgcc
      241 ggagcaaagc ttctactggc cggcggggcc ttgctgggcg cgatcgcgct tgccaaccga
      301 tacgctcggc cgcgggtgac gccgccgccg gcgcgccgcc gtagcgtgca ggtcgatggc
      361 gtgcggctgt tctatctcga gcagggcgaa ggcacgccga tcgtgctgct gcacggcaac
      421 ggcgcaatgg cggaggattt cgccgccagc gggattctgg accggctcgc ggtccggcat
      481 cgcgtgatcg ccttcgaccg gcccgggttt ggcgagagtg aacgcccgtc aacgcgaatc
      541 tggaccttcc ccgcgcaggc cgacctgctg cggcgagcgc tggtgcagat gggaatcgcg
      601 cggccggtgc tggttggcca ctcggccggc tgcctggcgg cgttgcacat ggccctgcac
      661 gatccacaag acactgcggc gctggtgctg ctgtcgggat tctacttccc ccggctacgc
      721 cctgacatgc tgatcacctc gctgtgggcg ctgccgggtc tgcgctggct ggcggccagc
      781 gcggcggcgc atctgttcgg ctggctggcg ctgcccggtt acctgcgcgt cgtcttcgcc
      841 cccaatcgcg tgcccgagcg attccgacgc agcttcccac tggctgccgt gctgcgcccc
      901 cgccatctca tggcgacgat cgccgatacc ccactgctgc tctgggaccc gatgaccttg
      961 ccgcgatgtc tcgaccggct gcgcatgccg gtgaccatca tgactggcac gcaggaccgg
     1021 attgtaatgg ggcgcctgca atcccgccgc ctgcaccagg ctgtcccggg gagccggctg
     1081 cacgcgttgg aagggatcgg ccacatgatc catcatgccg aacccgacgc tgtgattgct
     1141 gcgatcgagc aggcggcgga cgcagcgcag cgtcggctgg gcgctgatgg catcgcgccg
     1201 gagaacaagc cggtattctc ataaagccgg cttgctgccg gagaggggga gaggaacatg
     1261 gatccagttg tggaaaccgc cagcggaaga attcgcggga cggtgatcga gggcgttgcg
     1321 gccttcaaga gcatcccgta cggcgcgtcg accgtcggcg ccaatcgctt cctgccgccg
     1381 cgtccgccgc agccctgggc cggcatacgc gatgcgctgg actatgtcgg ccagtcgccg
     1441 cagtcgcgca ccggcatgcc gcagcgcgcc gaactggcga cgttctctgg cccgccggat
     1501 acgtccccgg aaaccgagga ttgcctgacc ctgcatgtct ggacgccatc ggccgatccg
     1561 gctgccaggc ggccggtgat ggtgtggctg catggcggcg cattctcctt cggctccgcc
     1621 aacagcctgc ggcttcaggg catgcggctg tgccggcggg gcgacgtggt gctggtgacg
     1681 gtgaaccagc ggctgaacat cttcggcttc ctcgacctgt cgcaattcgc cggaccggag
     1741 tacgcccagt cgggcaatgc cggcacgctg gacatgctcg cggcgctgca ctgggtgcgc
     1801 gacaacatcg cagcgttcgg cggcgatccg ggcaacgtca cgatcttcgg cgagtccggc
     1861 ggcgggggga aggtgagcac gctgatggcg atgccggcgg cgcggggcct gttccaccgc
     1921 gccattgtgc agagcggtgc cgcggtgcgg ctgcgcactg tcgagcgtgc agcgaaactg
     1981 accgacgcgg tgctgcatga actgggattg ggacgcgcgg cggtggccga attgcaggcc
     2041 gtgccgatgc agcggttgca ggcggcgatc gagccggctg tgaaagcttt ggggccgtct
     2101 ccctggccgc tgctggaccg ctatccgttc gggccggtgg tggatggcac gctgctgccg
     2161 cggcagccgt tcgatcccga tgcaacgccg gtttccgcgg atatcccgct gatcatcggc
     2221 gatacgacgc atgaggccgc cagcttcatc gcatacgaca accgcgtctg gtccggcacg
     2281 ctgaccgagg atgagctgcg gcgctgggtc gcgggcattg ccgggtccca cacggaccgg
     2341 gtgatcgcgc tgtaccggca gctgcatccc gcagccagtc cggcgcagcg gctgatcgcg
     2401 acgctgaccg actgcaattt ccgcctgcgc tcgctgctgc tggcggagcg caaggtgaag
     2461 caggcgggtg cgccggtctg gatgtactcc ttcgcctggc gcacgccgct gttcgacgga
     2521 cggctggggg cgccgcatgc gctggatgtg ccgttcacct tcgacacgct ggacttcacc
     2581 aacgcgaccg atcgcagtgc gggcgcacat gccctggccg gaacgatggc gactgcgtgg
     2641 gccaatttcg cgcgcagcgg cgtgcccggc gcggagggga tgccggaatg gccgaccttc
     2701 cggctggagg accgcgcgac catggtgctg gatgcaccgt gccgggtggc gcgcgatccc
     2761 gggcgggaag gccggctgct ctggcaggag atcgcggggt aacggacgca cggccggcgc
     2821 agcaggctcg cgtcggctgc cgccggtgcc tatctgaagg tggcgccggg acggcttgtc
     2881 cgggcgttcc ggatcagcat ggaccatcag cagactcgca ccgatcctcg ccccgccatc
     2941 cgccagcgtg cgctggcgct cggcttcgac gccatcggct tcgccgctgc cgaactcggc
     3001 gcggaggcgc gcgagcgcct gggcgcattc ctggctgcgg gacagcacgg ggatatgggc
     3061 tggctggccg accgcgccgc gcagcggtcg catccgcagg ccttgtggcc ggaggcgcgt
     3121 agcgtcatcg cccttggcct gtcctatgcg ccggaggacg atccgctggc ggcgctggat
     3181 cgtcctgatc gcggggcgat ctcggtgtat gcccgcaacc gcgattacca tgacgtgctg
     3241 aagggcatgc tgaagcacct ggcgcagttc atcgcgtcgc gctttgcggc tggggtgaag
     3301 gtgttcgtgg acactgcgcc ggtgatggaa aagccgctcg cggcacgtgc ggggctgggc
     3361 tggcagggca agcacaccaa tctggtttcg cgtcagcacg ggtcgtggct gttcctgggc
     3421 gagatctaca ccacgctggt tctgcagccc gacacgccgc acgccgaccg ctgcggcagt
     3481 tgcacgcgct gcctgtccgc ctgtcccacc gacgcgttcc cggcagcgta ccggctggat
     3541 gcgacccgct gcatctccta cctgacgatc gagcatcacg ggccgatccc gcacgaactg
     3601 cgtccgctga tgggcaaccg catctacgga tgcgacgatt gcctggcggt ctgcccgtgg
     3661 aaccgcttcg cgcgcggctc gccgcacgac aagctgcggg cgcgggaggc cttgagcgca
     3721 ccgcgtctgg cggaactggc gctgctggat gatgcagggt tccgcacaat gttttccggc
     3781 tcgccgatca agcgtatcgg ccgccatcgc ttcatccgca acgtgctgat cgcgatcggc
     3841 aattcgggcg atcccggatt gctgccggtg gcagcgcggc tgcgcggcga tgccgacccg
     3901 gtggtggcgg aagccgcgga atgggcctgc acacagttgc ggcgaagctg atcggccttg
     3961 cccggattgc ccgcgcgcgc ttgccgcaga cgcctatgcg gccgcggcca attggcgggg
     4021 gcggtagatc gcgatgtgcg tcactcgagc cagcggcgtg gattcgttcg ccagcaccag
     4081 cgcatcgatc tccacccact tatggccctt gtgctcgtag ttcttcgtca cgcgtgcccg
     4141 cacccccagc gagtcgccga catgtgcgac gccgaagttc tgcacggtgc tgcccatgtg
     4201 catccaggcc ggcagcacca cgttgtgcga cagcgcccag ttgcagcagc gcaggatggt
     4261 gcccggatgc acgataccct ccgcggcata gagcggggcg gtttcgcggg tgtccgcgag
     4321 atcctgcacc tggtagtccg gcgtgacggt cagcggggtc atgccaagcc agtcgccgac
     4381 gcgcagggat tgctcgtctg ccgggggacg gctggtgcgg gccggcaccg cgcggaaatc
     4441 ggcgagcgcg atcgccggt
//
LOCUS       DASSZH010000154         3875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_958069, whole genome shotgun sequence.
ACCESSION   DASSZH010000154 DASSZH010000000
VERSION     DASSZH010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958069"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1568)
                     /locus_tag="VFN42_03325"
     CDS             complement(45..1568)
                     /locus_tag="VFN42_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HET8995675.1"
                     /translation="MPASLPRSAAGNRNPWLIAVIVSIATFMEVLDTTIVNVALTHIA
                     GSLAISLDESTWILTSYLVANAIVLPISGWLANVLGRKRFYMICVAVFTASSVLCAAS
                     TSLTMIMLARTLQGAGGGGLAPTEQSIFADTFPPEKRALAFALYGLTVVTAPAAGPVL
                     GGYLTDAYSWHWCFLINLPIGLLSLTLVQLFVSEPSVLRQERRDLLAGGLRVDYIGFA
                     LVVLGFGSLQIMLDRYERDNGFSSSFITSLALVAAVSLLCLVVWELLHPQPVMNLRLF
                     RSRGFAISCVMMFMVFFTLISTTQLLPQLSQELLGYNSLEAGYALAYGGIATLIIMPI
                     AGFVTGRLIQPKWLILGALIGTGWGLLSNSGLDLDISFWTLAMARVRQVIWLPLLFIP
                     LSAVSYVGVPPERNNEASALQNLMRNLGGSIGVSVVTTLLQQRTQFHHERLASHITAY
                     NGYGWNVPLAHIARAVQTQASMMSYLDMFWLLGIVALCIWPLALFLPRMPKGAAPGH"
     gene            complement(1569..2690)
                     /locus_tag="VFN42_03330"
     CDS             complement(1569..2690)
                     /locus_tag="VFN42_03330"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HET8995676.1"
                     /translation="MDDPHAAPPPDAVSDTPPLSSADASHPPDPPGHWPRWLRRIAYA
                     TGAVIAIAVIAGGVIWWLNGQNFVTTSDAQIDGYITQLAPQVAGRVTQLLFTDNQHVT
                     AGQPLLKIDPRDYAVKLRQAEAGKASAQAQVTQARAQLVVQQTALDQDKANLRAAQAA
                     LTQAQQDYQRDKALRPRVISQQTMDQATEALRSAKAKQDAAQQAVQGAQAQIKAADAH
                     IQQAQAAVRQAGANVATAKLQLSYCTIVAPVSGTIGHRTVAVGDYVNPGQALFAIVQD
                     QVWVTADFKETQLAAIKPGDPVNISVDAVPGITFHGKVQGFQPGTGSIFSVLPAENAT
                     GNYVKVVQRVPVRIAFDDKRVEQYRLLPGESVEPAIRVH"
     gene            complement(2841..3170)
                     /gene="erpA"
                     /locus_tag="VFN42_03335"
     CDS             complement(2841..3170)
                     /gene="erpA"
                     /locus_tag="VFN42_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HET8995677.1"
                     /translation="MQQFAVTERAASRIAEIVAAEGRDAALRVAVLAGGCSGFQYKFE
                     LDDARQPDDLVIERAGAKVVVDPVSLDLLAGGELDFADELMGSYFAVRNPNAKSACGC
                     GTSFSVD"
     gene            3298..>3875
                     /gene="dgt"
                     /locus_tag="VFN42_03340"
     CDS             3298..>3875
                     /gene="dgt"
                     /locus_tag="VFN42_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019091133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HET8995678.1"
                     /translation="MTLAPYAVQPATSRGRLYPEPESPTRSPWQRDRDRIIHSSAFRK
                     LQYKTQVFVNHEGDFFRTRLTHTIEVAQIARSIARALNLNEDLTEALALAHDLGHPPF
                     GHAGEEALNLAMKPFGGFDHNAQSLRVVTLLESRYAGWDGLNLTWDTLEGLAKHNGPL
                     RNPPAYIADYNDKFRLELSTHASAEAQVAALA"
BASE COUNT          662 a         1280 c         1321 g          612 t
ORIGIN      
        1 gcgatctctg cctggccggc ggcgaggaac cgctcgtctg agagtcagtg tcccggcgcg
       61 gcgcctttcg gcatacgcgg caggaacagc gccagcggcc agatgcacag cgccacgatg
      121 ccgagcagcc agaacatgtc caggtagctc atcatcgagg cctgggtctg caccgcgcgg
      181 gcaatgtgcg ccaacggaac gttccaaccg tagccgttgt acgccgtgat gtgggaggcc
      241 agccgttcgt gatggaactg ggtgcgctgc tgcagcagcg tggtcaccac cgagacgccg
      301 atgctgccgc ccaggttgcg catcaggttc tgcagggcgg atgcctcgtt gttgcgttcc
      361 ggcggcacgc cgacatagga caccgcgctc agcggtatga acagcagcgg cagccagatc
      421 acctggcgga cgcgtgccat ggccagggtc cagaagctga tgtccaggtc caagccgctg
      481 ttcgacaaca ggccccagcc ggtgccgatc agcgcgccca ggatcagcca tttcggctgg
      541 atcagccggc cggtgacgaa tccggcgatg ggcatgatga tcagcgtggc gatgccgcca
      601 taggccaggg cgtaccccgc ttccagcgaa ttgtagccca gcagttcctg ggaaagctgc
      661 ggcagaagct gggtggtgct gatcagggtg aagaacacca tgaacatcat cacgcaggag
      721 atcgcgaaac cgcgcgagcg gaacaggcgc aggttcatca ccggctgggg atgcagcagc
      781 tcccagacca ccaggcacag caggctgacc gccgcgacca gcgccaggct ggtgatgaag
      841 ctggaggaga agccgttgtc ccgctcatag cggtccagca tgatctgcag cgaaccgaac
      901 cccagcacca ccagcgcgaa gccgatgtaa tccacccgca atccgccggc cagcaggtcg
      961 cgccgctcct gccgcagcac cgacggttcg ctgacgaaga gctgcaccag ggtcagcgac
     1021 agcaggccga tcggcaggtt gatcaggaag caccaatgcc aggaataggc atcggtcaga
     1081 tagccgccga gcaccgggcc cgcggcgggt gcggtcacca cggtcaggcc gtagagcgcg
     1141 aaggccagcg cgcgtttttc cggcgggaag gtatcggcaa agatcgactg ttcggtgggc
     1201 gccaggccgc cgccgccggc gccctgcagc gtccgcgcca gcatgatcat ggtcagcgag
     1261 gtggaggcgg cgcacagcac gctgctggcg gtgaacaccg cgacgcagat catgtagaag
     1321 cgcttgcgtc ccagcacatt ggccagccag ccgctgatcg gcagcacgat cgcattggcc
     1381 accaggtagc tggtgaggat ccaggtggat tcgtccaggc tgatggccag gctgccggcg
     1441 atatgcgtca gtgcgacgtt cacgatggtg gtgtccagca cctccatgaa ggtcgcaatg
     1501 gagacgatga cggcgatcag ccaggggttg cggttgccag cggccgaacg gggcagggat
     1561 gcaggcattc agtgcacccg gatcgcgggt tcgaccgatt cacccggcag cagccgatac
     1621 tgctccaccc gtttgtcgtc gaaggcgatt ctgaccggca cgcgctgcac caccttcacg
     1681 tagttgccgg tggcgttctc ggccggcagc acgctgaaaa tggagccggt gccaggctga
     1741 aaaccctgca ccttgccgtg gaaggtgatc ccgggcaccg catctacact gatgttcacc
     1801 ggatcgcccg gcttgatcgc ggcgagctgg gtctccttga aatcggcggt cacccagacc
     1861 tggtcctgca cgatggcgaa cagcgcctgg ccgggattga cgtagtcgcc caccgcgacc
     1921 gtgcggtggc cgatcgtgcc gctgaccggc gcaacgatcg tgcaatagga aagctgcagt
     1981 ttcgctgtcg ccacattggc cccggcctgc cgcaccgcag cttgcgcctg ctggatgtgc
     2041 gcgtcggccg ccttgatctg cgcctgggcg ccttgcaccg cctgctgcgc cgcatcctgc
     2101 ttggccttgg cggagcgcag cgcttccgtg gcctggtcca tcgtctgttg cgagatcact
     2161 ctcgggcgca gcgccttgtc gcgctgataa tcctgctgcg cctgggtcag cgcggcctgg
     2221 gcggcccgga gattggcctt gtcctggtcc agcgcggtct gctgcaccac cagctgggcg
     2281 cgcgcctggg tgacctgggc ctgggcgctg gccttgccgg cttccgcctg ccgcagcttc
     2341 acggcgtaat cgcgcgggtc gatcttcagc agcggctggc cggcggtgac atgctggttg
     2401 tcggtgaaca gcagctgggt gacccggccc gccacctgcg gcgcgagctg ggtgatatag
     2461 ccgtcgatct gcgcatcgct ggtggtgacg aaattctgtc cgttcagcca ccagatcacg
     2521 ccgccggcga tcacggcaat ggcgatcacc gcgcccgtgg cataggcgat gcgccggagc
     2581 cagcgcggcc agtgtccggg cggatcaggc ggatgcgatg cgtccgcgga agacagtggg
     2641 ggggtgtcgg acaccgcgtc ggggggcggc gcggcgtgcg ggtcatccat gcggcagcct
     2701 cggcaatcgg tttgcgcccg tccaccccgc tgccattacg atgcgcatgc caccagggtt
     2761 cgcgtatcac gcaggggtga gacgcggggc gttccgattc tcgggtcggg tttgcgcttc
     2821 tggctgccgc ctgggtggcg tcagtccacc gaaaagcttg tgccgcagcc gcaggccgac
     2881 ttggcgttcg gattgcgcac ggcgaagtag ctgcccatca gctcgtcggc gaaatccagt
     2941 tcgccaccgg ccagcaggtc gaggctgacc gggtctacca ccactttggc gccggcgcgc
     3001 tcgatcacca ggtcgtccgg ctgccgggcg tcgtccagct cgaacttgta ctggaagcct
     3061 gaacagccgc cggccagcac ggcaacgcgc agggcggcgt cccggccttc ggcggcgacg
     3121 atctcggcga tgcgggaggc ggcccgctcg gtcacggcga actgttgcat gctggggaag
     3181 atagggggga tggcgtggga attgaagtgt cgagcggggg ccagcccagg gggggcggca
     3241 tgctgacgga aagcgccgcg attgaacccc ccaccgccgc agtgtaatcc cggcgccatg
     3301 accctggccc cgtacgccgt gcagccggcg accagccgag gccggctcta cccggagccg
     3361 gaatccccca cgcgctcgcc gtggcagcgc gaccgcgacc ggatcatcca ttcctccgcc
     3421 ttccgcaagc tgcagtacaa gacccaggtc ttcgtcaatc atgaaggcga tttcttccgc
     3481 acccggctga cccacacgat cgaggtggcg cagatcgccc gctccatcgc ccgcgcgctg
     3541 aacctgaacg aggacctgac cgaagcgctc gccctggcgc acgatctcgg tcacccgccg
     3601 ttcggccatg ccggggagga ggcgctgaac ctggcgatga agccgttcgg cggcttcgac
     3661 cacaacgccc agagcctgcg cgtggtcacg ctgctggaga gccgctatgc cggctgggac
     3721 gggctgaacc tgacctggga cacgctggaa ggactggcca agcacaacgg cccgctgcgc
     3781 aacccgcccg cgtatatcgc cgattacaac gacaagttcc gcctggagct ttccacccat
     3841 gcatccgccg aagcccaggt ggcggcgctg gccga
//
LOCUS       DASSZH010000155         4117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_310441, whole genome shotgun sequence.
ACCESSION   DASSZH010000155 DASSZH010000000
VERSION     DASSZH010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310441"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..2010
                     /gene="tkt"
                     /locus_tag="VFN42_03345"
     CDS             31..2010
                     /gene="tkt"
                     /locus_tag="VFN42_03345"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET8995679.1"
                     /translation="MRQLANCIRALAMDAVQQANSGHPGMPMGMADIATVLFSDFLQF
                     DAADPHWLDRDRFLISNGHGSMLLYALLYLTGYHDMPIEQIEKFRQIDSRTPGHPEYH
                     HSDGIETTTGPLGQGLANSVGMALAERIMNARFGDDLVNHFTYVFMGDGCLMEGISHE
                     AISLAGHLKLGKLIAFFDNNSISIDGPTGLAVSDDQTERFRAAHWHVQEIDGHDTDAI
                     RDAIRAAQAVTDRPSMISCRTVIGFGLPTKAGTQKAHSDAPGAEEIAGARKLLGWDSP
                     PFEIPADLLEAWRKIGAKGRDARMAWAGRVKAAPDGKRQDFERRTRGELPPGWRKAIA
                     DARQEFLASGKDLATRAASGAVLNHLFDAIPELLGGSADLTPSNNTKAKNQVEIRPGA
                     YGGSYVHYGVREHGMAAAMNGIALHGGLIPYGGTFLTFSDYARPAIRLAAMMEIRTIF
                     VMTHDSIGLGEDGPTHQPVEHLAALRAIPHLAVYRPGDPIETAECWEAILDTPRQAAL
                     IALSRQGMPLLRREPGSENRSARGGYVLAEAEGGPRKLTLMATGSELHLAMQARSVLQ
                     QEGVPTAVVSLPCFERFEAQDAAYRRSVLGEESVARVAVEAGVESCWGRYLGFAGRFV
                     GMHSFGASGKGSDVFRKFGITADAVVAAARKAVAG"
     gene            2147..2428
                     /locus_tag="VFN42_03350"
     CDS             2147..2428
                     /locus_tag="VFN42_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HET8995680.1"
                     /translation="MSWHIPWRLGSVPQSNPAPRHVRLFRNNRNQAIRIPVEFELPGS
                     EAILSRDGDRLIIEPVRRRGLAALLDSWQPLTEDFPEAADAPPAPEDIF"
     gene            2440..2826
                     /locus_tag="VFN42_03355"
     CDS             2440..2826
                     /locus_tag="VFN42_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003630489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HET8995681.1"
                     /translation="MLDTNIVSELIRNPAGKAAQRARALADSACVSVIVAAELRYGCA
                     GKGSPQLLRRVEAFLAEVPVLPFDVPADAAYGDIRAELEAAGQPIGANDLLIAAHARA
                     LAATVVTANVAEFARVPGLAVENWLE"
     gene            2995..3561
                     /locus_tag="VFN42_03360"
     CDS             2995..3561
                     /locus_tag="VFN42_03360"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET8995682.1"
                     /translation="MGKTALTREPETRMSRQEYRAWLDQQPDGRFERIQGVVVAMAPE
                     RAEHALCKARIWQALDRAVREAGLPCEVYPDGMTVEVGDSDYEPDAVLRCGAALGGKA
                     IAVPDPLIIVEVLSPTTSGIDRAWKLREYFRLPSLRHYLIVRPDMPHMVRHTMLPTGE
                     VETRVFTTGEIALAPPGIGITVEEVYTR"
     gene            complement(3647..>4117)
                     /locus_tag="VFN42_03365"
     CDS             complement(3647..>4117)
                     /locus_tag="VFN42_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HET8995683.1"
                     /translation="RPAVAPPLATTPELAEEPATFERPILERVVSRPFRDAAFSAAVR
                     SAYENTCAMTGLKIINGGGRAEVQAAHIRPVAESGPDSVRNGLALSGTIHWMFDRGLL
                     SLDDDLTILVAKGRVPDTVDRLINPDRRLRAPPRPELRPHRQFLRFHRDSIFKG"
BASE COUNT          659 a         1338 c         1471 g          649 t
ORIGIN      
        1 ggcgcgcttc cgtcgccccg cgacccgcat ctgcgccagc tcgccaactg catccgtgcg
       61 ctggcgatgg atgcggtgca acaggcgaac agcgggcatc ccggcatgcc gatgggcatg
      121 gccgacatcg ccaccgtgct gttctccgac ttcctgcagt tcgatgccgc cgatccgcac
      181 tggctggacc gcgaccggtt cctgatctcc aacggtcacg gctcgatgct gctgtatgcc
      241 ctgctgtacc tgaccggcta tcacgacatg ccgatcgagc agatcgaaaa attccgccaa
      301 atcgacagcc gcacgccggg ccatccggaa taccaccact ccgacggcat cgagaccacc
      361 accggcccgc tcggccaggg gctggccaat tccgtcggca tggcgctggc agagcggatc
      421 atgaacgcgc ggttcggtga cgatctggtg aaccacttca cctatgtgtt catgggcgac
      481 ggctgcctga tggaaggcat cagccatgag gcgatctcgc tggccggcca cctgaagctg
      541 ggcaagctga tcgccttctt cgacaacaac agcatctcga tcgacggccc gaccggcctg
      601 gcggtgtcgg acgaccagac cgaacggttc cgcgcggcgc actggcacgt gcaggagatc
      661 gacgggcacg acaccgacgc gatccgtgac gcgatccgcg ccgcgcaggc ggtgacggac
      721 cggccgtcga tgatctcctg ccgcaccgtc atcggcttcg gcctgccgac caaggccggc
      781 acgcagaagg cgcacagcga cgcgccgggt gccgaggaga tcgccggcgc ccgcaagctg
      841 ctgggctggg actcgccgcc cttcgagatt ccggccgatc tgctggaggc ctggcgcaag
      901 atcggcgcca aggggcgcga cgcacgcatg gcatgggccg gccgggtgaa ggccgcgccg
      961 gacggcaagc ggcaggattt cgagcgccgc acgcgcggcg agttgccgcc cggctggcgc
     1021 aaggccatcg ccgacgcgcg gcaggaattc ctcgcctccg gcaaggacct ggccacccgc
     1081 gcggccagcg gcgccgtgct gaaccacctg ttcgacgcca tccccgaact gctcggcggc
     1141 tccgccgacc tgacgccgtc caacaacacc aaggcgaaga accaggtgga gatccgcccg
     1201 ggcgcctatg gcggctccta cgtgcattac ggcgtgcggg agcacggcat ggcggcggcg
     1261 atgaacggca tcgcgctgca tggcgggctg atcccgtacg gcggcacgtt cctgaccttc
     1321 tccgactacg cccgcccggc gatccggctg gcggcgatga tggagatccg caccatcttc
     1381 gtgatgacgc atgattcgat cggcctgggc gaggacggac cgacgcacca gccggtggag
     1441 cacctggccg cgctgcgtgc catcccgcat ctggcggtgt accgtcccgg cgacccgatc
     1501 gagaccgcgg aatgctggga ggcgatcctg gacacgccgc gccaggcggc gctgatcgcg
     1561 ctgtcgcgcc agggcatgcc gttgctacgc cgcgaaccgg gcagcgagaa ccgcagcgcc
     1621 cgtggcggct acgtgctggc ggaggcggag ggcgggccgc gcaagctgac gctgatggcg
     1681 accggctccg aactgcatct ggcgatgcag gcgcgcagtg tgctgcagca ggagggtgta
     1741 ccgaccgcgg tggtgtcgct gccgtgcttc gagcgcttcg aggcgcagga cgcggcgtat
     1801 cgccgctcgg tgctggggga ggagagcgtg gcgcgcgtgg cggtggaagc gggggtggaa
     1861 agctgctggg ggcgctatct gggctttgcc gggcgcttcg tcgggatgca cagcttcggc
     1921 gcgtccggca agggcagcga cgtgttccgc aagttcggca tcaccgcgga cgcggtggtg
     1981 gcagcggcgc ggaaggcggt ggcggggtag cgaacggcac gttcggagcg aaggctcctc
     2041 cccctcccct tgcgggaggg gggcgggggg agggggcgct gaaggttgct cggggaatgg
     2101 ccaaccgccc cctcccttgg cttgaaccca gcattcctcg cggcatatgt cttggcatat
     2161 accatggagg cttggttctg tgcctcagtc caacccagcc cctcgtcatg tccggttgtt
     2221 ccgcaacaac cgcaaccagg ccattcgcat cccggtggaa ttcgagttgc ctggcagcga
     2281 ggctatcctc agccgcgacg gcgaccgtct gatcatcgag ccggtgcgcc gcagggggct
     2341 ggccgcgctg ctggatagct ggcagcccct gacggaagat ttcccggagg ccgctgatgc
     2401 gccaccggcg cccgaggaca tcttctgact gtgcggtaca tgctggacac gaacatcgtc
     2461 agcgagctga tccgcaaccc cgccggaaaa gctgcccagc gtgcaagggc gttggcggac
     2521 tcagcgtgtg tcagcgtcat cgtggccgcc gagctgcgct atggctgcgc ggggaaagga
     2581 tcgccgcagt tgctgcgccg ggtcgaggcc ttcctggcgg aagtcccggt tcttcccttc
     2641 gatgttcccg cggatgcggc gtacggcgac attcgcgcgg aacttgaggc cgccggccag
     2701 ccgatcggag cgaacgacct gctgatcgcc gcacatgccc gtgctttggc ggcgacggtc
     2761 gtgacggcaa acgtggccga gttcgcacgg gttcctggac tggctgtgga gaattggctg
     2821 gaatgatcag cggtccaggc tgactccggg ttgggaatgt gctggcttgg agcgctacgt
     2881 tgccatctcg ccaaagcctt tgcggatgac gccgggaaga gctgcccatc atcgcgactg
     2941 gcgaggggcg ggcatcgctg gtaccttggc cggcgaagcc tggagttcgc gcccatgggt
     3001 aagacagcgc tgacccgcga acccgaaacg cgcatgtcgc ggcaggagta tcgtgcctgg
     3061 ctggatcagc agccggacgg gcggtttgag cgcatccagg gcgtcgtggt ggcaatggcc
     3121 ccggagcggg ccgagcacgc tctgtgcaag gcgcgcatct ggcaggcgct tgaccgcgcc
     3181 gtgcgcgagg ccggcctgcc atgcgaggtc tatcccgatg gcatgacggt tgaggttggt
     3241 gacagcgact acgagcccga tgctgtcctg cgctgcggcg ctgcgctggg cggcaaggcg
     3301 attgccgttc cggacccgct gatcatcgtg gaagttctgt ctcccacgac gagcggcatc
     3361 gatcgtgcct ggaaactgcg ggagtatttt cgcctgccct cgctgcggca ctatctgatc
     3421 gtgcgacccg atatgccgca tatggtgcgg cacacgatgc tgccgacggg agaggtggaa
     3481 acccgggtgt tcaccactgg cgaaatcgcg ctggcgccgc ccgggatcgg catcaccgtt
     3541 gaggaagtct acacgcggtg attgggtgtg gcctccggcg gacggcgggg cgactcaggg
     3601 attggcagtt tgaccgcgcc gccaagccgc agcgggaggc aggcgctcaa cctttgaata
     3661 tgctgtcccg gtggaagcgc aggaactgcc ggtgcgggcg gagttcgggg cgcggcggcg
     3721 cgcgcagccg gcggtccgga ttgatcagcc ggtccaccgt gtccggaacg cggcccttcg
     3781 ccaccaggat cgtcaggtcg tcgtccagcg acagcagccc gcgatcgaac atccagtgga
     3841 tcgtgccgga cagcgccagg ccgttgcgga ccgaatccgg accggactcg gcaaccggcc
     3901 ggatatgcgc cgcctgcacc tccgcgcgtc cgccgccgtt gatgatcttc aggccggtca
     3961 tggcacaggt attctcatag gccgagcgta cggcggcgga gaatgcggcg tcgcggaagg
     4021 ggcggctgac cacgcgttcg agaatgggcc gttcaaatgt cgcaggctcc tcggcaagct
     4081 ctggggtggt ggcaagcggc ggcgcaacgg ccggccg
//
LOCUS       DASSZH010000156         7281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_838619, whole genome shotgun sequence.
ACCESSION   DASSZH010000156 DASSZH010000000
VERSION     DASSZH010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7281
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838619"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VFN42_03370"
     CDS             <1..791
                     /locus_tag="VFN42_03370"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET8995684.1"
                     /translation="AHQAGGGAAAIVPAAGELLLSGSGDTEQASSADGRLLLVSACQT
                     RFGEHGGIAVWRLRGSRAWDQEERLLLRSAANLIRIVLEHEAIQREMVRQARTDPLTG
                     LLNRRAFLEEIERHIERLDRESLSGTLLFVDLDNFKPVNDQLGHELGDEVLLHTALLL
                     RGTVRPTDLVARFGGDEFAVWLNGADHLTAAERAEQLRTQAPRELRELVGDGPPLSLS
                     IGIASRSPGSHEPVESLMRRADNAMYEVKRQGRGHWRVAPEAQV"
     gene            835..2067
                     /locus_tag="VFN42_03375"
     CDS             835..2067
                     /locus_tag="VFN42_03375"
                     /inference="COORDINATES: protein motif:HMM:NF021603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HET8995685.1"
                     /translation="MSRPPQQPQRLQDADEATRVRLGASQATPPETLRQLAADASATV
                     RAALAMNPAAPAQANHLLAHDADERVRTLLARKLASLAPHLSCDAQAALQLHAHETLL
                     ALVHDEAVRVRAAIADAVKEMPDAPRSVILQLAQDASVMVSEPVIRLSPLLGTEDLLA
                     LLSGAPPPAMLLAVSRRQGIEAAVSDAIAASADSAAIQALLSNPSAHIREATLDALIA
                     RAVGHADWHEPLVRRPALSARSARALSEIVATHLLEVLAARSDLPARLAEELRVRLAA
                     RLAPLPAMLPPPDDQLTEDALTRARNTAAQGELTEAMLLQAAQRGDARLVSALLAVAT
                     SLPISVIDRAATLRSAKGLVSLTWKAGFTMRCATALQMMLARLAPDAILSAGPAGGFP
                     LAVEEMRWQLDFLERMGR"
     gene            complement(2212..2910)
                     /locus_tag="VFN42_03380"
     CDS             complement(2212..2910)
                     /locus_tag="VFN42_03380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8995686.1"
                     /translation="MPVLTAGRVLPGLHRHVLLAGLLALGIGAAGFPARSQAATLPAP
                     AAAQSAPASTPDGAIASFETDLLNVMKAGNTASFVQRFNMLAPAVDRTFDLETILRNS
                     VGFKWATLPEEQRQKLLDTFRRYTIATWVANFSSWSGQQFKVSTELRHVANQVVVPTY
                     LVPKSGSPTNLSFVMKQVGAAWKVVDVLADGSISRVAVQRSDFQGLLSSGGVPALVAS
                     LQNKIASLSDGSLA"
     gene            complement(3080..3865)
                     /locus_tag="VFN42_03385"
     CDS             complement(3080..3865)
                     /locus_tag="VFN42_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8995687.1"
                     /translation="MVTGARLPLTVDLSGMRVAITAGANGIGRVMADSFVDCGARVYV
                     SDVDDDALAASGHPGMRADAGNPAECEAFVDAAIARFGGLDVLVNNAGIAGPTAPVEQ
                     VTPEQLDATLRIDLASMFHCSRRAIPALRAAGGGAIINLSSAAGRFGFPLRAPYSAAK
                     WGVVGFTKSLSIELGPDGIRVNAILPGPVDGPRIRAVIKAKAEAAGISENEMTDRTVG
                     TTSLRCFVTQQDIANMALYLASPFGATISGQAMAIDGDMQTTM"
     gene            3975..4907
                     /locus_tag="VFN42_03390"
     CDS             3975..4907
                     /locus_tag="VFN42_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET8995688.1"
                     /translation="MDSADLRVFEAVARTGGIGRAAIALHTVQSNVTARIRLLEEELG
                     TPLFIRHSRGVSLAPAGRRLLPYAERVRDLLAQARRAAADDGTPQGPLALGSLETAAA
                     LRLPRILAGFAQAHPAVDLSLVTGTSAELIEQVLQARLDGALVVGPVRHPELTETVVF
                     HEEMVLVTASNVAGWDDLPRRSGLKLVVFRAGCTYRAQLEAMLAERGVVGLRRIEFGT
                     LEGILGCVAAGIGITLLPRGVIGAAWRDGPLALHPLPEAQRWADTVFIRRADAFPSSA
                     VAAFVDALTAAAQRAEAITRIDGSDQNKPFFAVA"
     gene            4948..6135
                     /locus_tag="VFN42_03395"
     CDS             4948..6135
                     /locus_tag="VFN42_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HET8995689.1"
                     /translation="MPQVRTTHAALAGLSASLVGIGLARFAYTPLIPALIGAHWFAPA
                     AVIYLGAANLAGYLAGALAARPLAAWQPAGGVLRGMMLLATASFFASAAPVSLAWFGA
                     WRFAAGLAGGVIMVLAAPTILPHTDAKRRGLVGGLIFTGVGLGVAASGTLVPLLLRAG
                     LPATWIGLGGISAVLTLLAWGGWPHDPAHSPGARHGALPAWSRPVRGVLLEYGLNAVG
                     LVPHMVFLVDFVARGLDQGLAAGSAYWVVYGIGALGGPLLAGTLADRIGFRAALRAGF
                     VVQAAAVALACLDGTLWLALSALIAGAFTPGVVPLALGRLHELLPGHPAAQRRAWGHA
                     TMSWALFQAAAAYLDSWLFAQTGSYRLLFALGCGALVLALATDLLAGYSAAARAASGS
                     SAT"
     gene            complement(6090..6902)
                     /locus_tag="VFN42_03400"
     CDS             complement(6090..6902)
                     /locus_tag="VFN42_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET8995690.1"
                     /translation="MRSRRITKPLIGISCCTKQFGAFGMPNHAASDTYVRATDQVVGG
                     APVLIPANGPTADIETLLEHLDGIILTGSRSNVQPSLYDGPPHAEGTWEDPARDGTTL
                     PLIRAAVVRGVPLLAICRGFQELNVALGGSLHQRLQDLPDRFDHSTPMQPSHRVRTGK
                     AHNVRLTQGGWLHRLAGAGEIAVNSLHNQGINRLAPGLVVEAVAPDGTIEGVRVVSTP
                     TGPVAGYAVGVQWHPEYDWPRDAVSRAIFADFAEAVRAYVADEPLAALAAAE"
     gene            complement(7087..>7281)
                     /locus_tag="VFN42_03405"
     CDS             complement(7087..>7281)
                     /locus_tag="VFN42_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019090204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HET8995691.1"
                     /translation="VDGVRVNTEDGWWLLRASNTQAVLVARAEAKTPEGLERLKAALV
                     RQLEASGLPAPDFSGSHAGH"
BASE COUNT          999 a         2576 c         2606 g         1100 t
ORIGIN      
        1 tggcgcatca ggccggcggg ggcgcggcgg ccatcgtgcc ggccgcgggg gagctgctgc
       61 tgtccgggtc cggcgacacg gaacaggcca gcagcgcgga cgggcgtttg ctcctggtgt
      121 ccgcctgcca gacccggttc ggcgagcatg gcggcatcgc tgtctggcgc ctgcgcggca
      181 gccgcgcctg ggaccaggag gagcggctgc tgctgcgctc cgccgccaat ctgatccgga
      241 tcgtgctgga gcacgaagcc atccagcgcg aaatggttcg gcaggcccgc accgacccgt
      301 tgaccgggct gctcaaccgc cgcgccttcc tggaggagat cgaacggcat atcgagcggc
      361 tggaccgcga aagcctttcc ggcaccttgc tgttcgtcga cctggacaat ttcaagccgg
      421 tcaacgacca gctcggacac gagctggggg acgaggtgct gctgcacacc gccctgctgc
      481 tgcgcggcac ggtgcgcccg accgatttgg tcgctcggtt cggcggggac gagttcgcgg
      541 tctggctgaa cggtgccgac cacctgaccg cggcggaacg cgccgaacag ctgcggacgc
      601 aggcgccccg ggaattgcgt gagctggtgg gcgacgggcc gccgctgagc ctgtcgatcg
      661 gcatcgccag ccgcagcccg ggcagccatg aaccggtgga aagcctgatg cgccgtgccg
      721 ataacgccat gtacgaggtg aagcggcagg gccgaggcca ctggcgggtc gcacccgagg
      781 cgcaggtgtg atgcctcaca ctggcagtgc cgccaccagg cacgggaatt cagcatgagc
      841 cggccgccgc agcaaccgca gcggctgcag gacgccgacg aggcgacccg ggttcgtctt
      901 ggcgccagcc aggccacgcc gccggagacg ctgcggcagc ttgcggcgga tgcctcggcg
      961 acggtgcgcg ccgcgctggc gatgaacccg gcggcaccgg cgcaggcgaa ccatcttctc
     1021 gcccacgacg cggatgaacg cgtgcgcacc ctgctggcgc gaaaactcgc cagtctggcg
     1081 ccgcacctgt cctgcgacgc tcaggcggcc ttgcagctcc acgcgcatga aacgctgctg
     1141 gcgctggtgc atgacgaagc ggtgcgcgtg cgcgccgcca tcgccgatgc ggtgaaggaa
     1201 atgccggacg cgccacgctc ggtgatcctg cagctcgcgc aggacgcgtc ggtgatggtc
     1261 agcgagccgg tgatccgcct gtcgccgctg ctgggcaccg aagacctgct ggcattgctg
     1321 tccggcgcac cgccgcccgc catgctactg gcggtatcgc gccggcaggg catcgaggcc
     1381 gcggtttccg acgccatcgc cgccagcgcc gacagcgcgg cgatccaggc gctgctgagc
     1441 aacccctcgg cgcatatccg cgaggcgacg ctcgacgcgt tgatcgcccg cgcggtgggg
     1501 cacgccgact ggcacgaacc cctggtgcgc cgcccggccc tgtcggcgcg atccgcccgg
     1561 gcgctatcgg agatcgtcgc cacgcacctg ctggaggtgc tggccgcgcg cagcgacctg
     1621 ccggctcggc tcgccgagga actgcgagtc agactggccg cacggctggc gccgctgccg
     1681 gcgatgctgc cgccgccgga cgatcagctc accgaggacg cgctcacccg ggcgcggaac
     1741 acggccgcgc agggggaact caccgaagcg atgctgctgc aggcggcgca acgcggcgac
     1801 gcgcgcctgg tgtctgctct gctggcggtg gcaaccagcc tgccgatctc ggtgatcgac
     1861 cgtgccgcca cgttgcgcag cgccaagggc ctggtgagcc tgacctggaa ggcgggcttc
     1921 accatgcgct gtgccaccgc gctgcagatg atgctggccc gcctggcgcc ggacgcgatc
     1981 ctgtccgccg ggccggccgg cggctttccg ctcgcggtgg aggaaatgcg ctggcagctc
     2041 gacttcctgg agcggatggg acgctagagc ctgcccggtg cggcaagaag cacgccgcag
     2101 gctccaacac tttgtctgat cgcgtgattc acggcttcgc cgattccgcc cgaaccgatc
     2161 atgctccagc cggccagcgt cgccggtgac cggacctgtt cgccgcgacc gctatgccag
     2221 gctgccgtcg gacaggctgg cgatcttgtt ttgcaggctt gccaccaggg ccggcacgcc
     2281 accgctcgac agcagcccct ggaagtcgga gcgctgcacc gcgacgcggc tgatcgagcc
     2341 atccgccagc acgtccacca ccttccaggc agcgccgacc tgcttcatca cgaagctcag
     2401 attggtcggc gaaccgctct tcggcaccag ataggttggc accaccacct ggttggccac
     2461 gtgacgcagt tcggtggaaa ccttgaactg ctggcccgac cagctgctga aattcgccac
     2521 ccaggtggcg atggtgtagc ggcggaacgt gtccagcagc ttctgccgct gctcctcggg
     2581 caaagtggcc catttgaagc cgaccgaatt gcgcaggatg gtttccaggt cgaacgtccg
     2641 gtccaccgcc ggggcgagca tgttgaagcg ctgcacgaag ctggcggtgt tgccggcctt
     2701 catgacgttc agcaggtcgg tttcaaacga cgcaatcgca ccgtcgggcg tgctggcggg
     2761 ggcggactgc gccgcggccg gcgccggcag cgtggcggct tggctgcgcg ccgggaatcc
     2821 ggcggcgcca atacccagcg ccagcaggcc ggcgagaagc acatggcgat gcaggccggg
     2881 cagcacccgg ccagcggtca gaacgggcat cctttggtct cctccgtctc gatgcacgga
     2941 tggccatgcc ggcccggaag ccgccggcaa aatcggaccg gccatccgtc gtggtcaacg
     3001 cacgaacacg agcgccggat tgccaccgtt cgccatcggc aagatgtctg tcgcagggcg
     3061 cggcgcgggc cgctcgctac tacatcgtcg tctgcatgtc gccatcaatc gccatcgcct
     3121 gcccgctgat cgtcgcgccg aacggactgg cgagatacag cgccatgttg gcgatatcct
     3181 gctgcgtcac gaagcagcgc aggctggtcg tgccgacggt gcggtccgtc atctcgttct
     3241 cggaaatgcc ggcggcctcg gccttggcct tgatcaccgc acggatgcgc ggcccatcca
     3301 ccggccccgg caggatggcg ttcaccctga tgccatccgg cccgagttcg atcgacagcg
     3361 acttggtgaa tcccaccacg ccccatttgg cggcggaata gggcgcgcgc agcggaaagc
     3421 cgaagcgccc ggcggcggag ctcagattga tgatcgcgcc gccgcccgcg gcccgcagcg
     3481 ccggaatggc ccgccgcgag cagtggaaca tgctggccag gtcgatgcgc agcgtggcgt
     3541 cgagctgctc cggcgtgacc tgctccaccg gggcggtggg accggcgatg ccggcattgt
     3601 tcaccagcac gtccagcccg ccgaatcggg cgatggcggc gtccacgaag gcctcgcact
     3661 ccgccggatt gccggcatcg gcgcgcatgc ccggatggcc gctggcggcc agcgcatcgt
     3721 cgtccacatc ggagacgtac acccgtgcgc cgcaatccac gaaactgtcg gccatcaccc
     3781 ggccgatgcc gttggcgccg gcggtgatcg ccacccgcat cccactcaaa tccaccgtca
     3841 ggggcaggcg tgctccggtc accatgggcg atctcctgtc tttgcgccgc gcccagtctc
     3901 gcgatgccga cgggtggcgg caaggccagc cggcacgttg ccatccggac cgcagggtct
     3961 ttactgtcgc cgccatggac tcagcggacc tgcgcgtctt tgaggcagtg gcacgcaccg
     4021 gcggtatcgg ccgcgcggcg attgcgttgc ataccgtaca atccaacgtc accgcacgca
     4081 tccggctgct ggaggaggaa ctcggcaccc cgctgttcat ccggcacagc cgcggcgtca
     4141 gccttgcccc ggccgggcgc cgcctgctgc cctatgccga acgcgtgcgc gacctgctgg
     4201 cgcaggccag gcgcgcggcg gcggatgacg gcaccccgca aggccccctg gcactgggat
     4261 cgctggagac cgccgccgcg ctgcggctgc cgcgcatcct ggcgggcttt gcccaggcgc
     4321 atccggcagt ggacctgtcc ctcgtcaccg gcacctcggc cgagctgatc gagcaggtgc
     4381 tccaggctcg gctcgacggc gcactggtgg tcgggccggt gcgccacccg gaactgaccg
     4441 agacggtggt gttccacgag gagatggtgc tggtcaccgc gagcaatgtc gccggctggg
     4501 acgacctgcc ccgccgcagc ggcctgaagc tggtggtgtt ccgcgccggc tgcacgtatc
     4561 gggcccagct ggaggcgatg ctggcggagc gtggcgtggt tgggctgcga cggatcgagt
     4621 tcggcacgct ggagggcatc ctgggctgcg tcgccgccgg catcggcatc acgctgctgc
     4681 cgcgcggcgt gatcggcgcg gcgtggcgcg acgggccgct ggccctgcat cctctgccgg
     4741 aagcgcagcg ctgggccgac accgtgttca tccggcgcgc cgacgcgttc ccctccagtg
     4801 cggtggccgc cttcgtggat gcgctgactg cggcggcaca gcgcgcggaa gccatcaccc
     4861 ggatcgatgg ctcggatcag aacaaaccat ttttcgccgt cgcctagacg gcccagcttc
     4921 acccgggaac aaccgggagc atccccgatg ccacaggtcc gcaccacgca tgccgcgctg
     4981 gcgggattga gcgccagcct ggtcggcatc ggcctcgccc gcttcgccta cacgccgctg
     5041 attccggcgc tgatcggcgc gcactggttc gcgccggccg cggtgatcta cctgggcgcc
     5101 gccaatctgg ccggctatct ggcgggggcg ctggccgccc gcccgctggc cgcgtggcag
     5161 ccggcgggcg gcgtgttgcg gggcatgatg ctgctggcca ccgccagctt cttcgcctcc
     5221 gccgcaccgg tctccctggc gtggttcggc gcctggcgtt tcgcggccgg actggccggc
     5281 ggggtgatca tggtgctggc cgcgccgacc atcctgccgc acaccgatgc gaagcgccgc
     5341 gggctggtgg gcggactgat cttcaccggc gtggggctgg gcgtcgccgc gtccggcacg
     5401 ctggtgccgc tgctgctgcg ggcgggcctg ccggcgacct ggatcgggct gggtggcatc
     5461 tccgccgtgc tgaccctgct ggcctggggc ggctggcccc atgacccggc gcattcgccc
     5521 ggggctcggc acggcgccct gccggcctgg agtcgcccgg tgcgcggcgt gctgctggaa
     5581 tacgggctga acgctgtggg cctggtgccg cacatggtgt tcctggtgga tttcgtcgcg
     5641 cgcggcctgg accagggatt ggcggccggc tctgcttatt gggtggtgta cgggatcgga
     5701 gcgctgggcg ggccgctgct ggccggcacg ctggccgacc ggatcggctt ccgcgccgcg
     5761 ctgcgggccg gcttcgtggt gcaggcggcg gcggtggcgc tagcctgcct ggacggcacg
     5821 ctgtggctgg cgctctcggc gctgatcgcc ggcgccttca cccccggcgt ggtgccgctg
     5881 gcgcttggcc ggctgcacga gctgctgccc gggcatccgg cggcgcagcg gcgcgcctgg
     5941 gggcatgcca ccatgtcctg ggcgctgttc caggcggcgg cggcctatct ggattcctgg
     6001 ctgttcgcgc agaccggcag ctaccggctg ttgttcgcgc tgggctgcgg cgccctagtg
     6061 ctggcgctgg cgaccgacct gctggccggc tattccgcgg cggccagggc ggcgagcggc
     6121 tcgtccgcca cataggcgcg caccgcttcg gcgaaatcgg cgaagatggc gcgggacacc
     6181 gcgtcccgcg gccaatcgta ttccgggtgc cattgcaccc cgaccgcgta gcccgccacc
     6241 ggacccgtgg gggtggagac gacccgcacc ccctcgatgg tgccgtccgg cgccacggct
     6301 tccaccacca gcccgggggc cagccggttg atgccctggt tgtgcagcga gttcaccgcg
     6361 atctcgccgg cgccggccag ccggtgcagc cagccgccct gggtcagccg cacattgtgc
     6421 gccttgccgg tgcgcacccg gtgggagggc tgcatcggcg tggaatggtc gaatcggtcc
     6481 ggcaggtcct gcagccgctg gtgcagcgag ccgcccagcg cgacgttgag ctcctgaaag
     6541 ccgcggcaga tcgccagcag cggcacgccc cgcaccacgg cggcgcggat cagcggcagg
     6601 gtggtgccgt cgcgcgccgg gtcttcccag gtgccttcgg catgcggcgg cccgtcatac
     6661 aggctgggct gcacgttgga gcggctgccg gtgaggatga tgccgtccag gtgttccagc
     6721 agggtctcga tatccgcggt gggtccgttg gccgggatca gcaccggggc gccgcccacc
     6781 acctggtcgg tggcgcggac ataggtatcg gacgcggcat ggttcggcat cccgaaggcg
     6841 ccgaactgct tcgtgcagca gcttatgccg atcagaggct tggtgattcg tcgtgaccgc
     6901 atcggaagcg ccataattgg cgaccatggc ggaaaaaaga agcgaaatcc ccgccatggc
     6961 cgccatgtgc ccggcgcaga cgggcgagac tgctccccct cccctcgcgg gcttgggccg
     7021 cgaagcggac caaggatggg ggagggggag gagtccctac cttctcctct gtgcccggac
     7081 ctcccctcag tgcccggcgt ggctgccgga gaagtccggg gcgggcaggc cggaggcttc
     7141 caactggcgg accagcgcgg ccttcagccg ctccagcccc tccggcgtct tcgcctcggc
     7201 acgggccacc agcaccgcct gggtgttgga ggcgcgcagc agccaccagc cgtcctcggt
     7261 gttcacccgc acgccgtcca c
//
LOCUS       DASSZH010000157         2947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1077332, whole genome shotgun sequence.
ACCESSION   DASSZH010000157 DASSZH010000000
VERSION     DASSZH010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077332"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VFN42_03410"
     CDS             complement(<1..483)
                     /locus_tag="VFN42_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995692.1"
                     /translation="MVLRDVQPFRQLAAACVKGAACAITPEAEETFAPGTALILGGTP
                     VAAAHPHGDTKVFGADALARPDSSSGVVRMLARAKEDRLAWCWLLMVACVTGALCLPF
                     LHFATLSGDEGLLFDGVQRMLLGHTIYRDFFEFLPPGSFIVTAGWLWMFGDSYVCAQS
                     L"
     gene            490..1803
                     /locus_tag="VFN42_03415"
     CDS             490..1803
                     /locus_tag="VFN42_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET8995693.1"
                     /translation="MSDAVIASLAEQRNAILDRLKALLRIPSVSTDPAFAPHMQAARN
                     FLLQRLRDGGLHNVQLLDGGGQPAVFGEWLGAPGKPTLIVYGHYDVQPADPLELWDSP
                     PFEPTERTGRLPVNIKLFIEGEEESGSPSVRAIVERYRHLLDADAMLSADGGRASTTI
                     PTLGIGGRGITGLQFTLRTAKKDAHSGRYGGAARNALHDMAALIASLHDAQGRILVPH
                     YGDDAAPITNSARAAAAALPSDDAAFLAAIGGVRYGDPNYTVRELTTLCPTIEVNGMW
                     GGYTGAGGKTVLPAEAHAKITMRLAPGMDPARARQHLEAHLRAQVPHGVALQISAEKG
                     GTPAPTLPDDHPLLVTASRVQEQLHGRKPIPVRAGGTIPITAVFREMLGIDTLTYGLA
                     MPDEDVHAPNEFFRLSSFDEGLKSWPMLLTALGDLSAADFARFRH"
     gene            complement(2041..2757)
                     /locus_tag="VFN42_03420"
     CDS             complement(2041..2757)
                     /locus_tag="VFN42_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995694.1"
                     /translation="MSLSHLCLSLLALTPELSASLLSGLLAAVPVIPGAATPEQRKAL
                     LIQQMNEMQPRDLEEAMLASQFLAAWHQANACFDALEGLDPVSKEASRLRRDGLAMQR
                     SAIATQRALHRSRTRPMLQDADGGEPRPTVPVRLANPPRGRAAGQENPPVAAMPAPAN
                     RPTAAGFPVAQAVAEAYAKDPSLARLAQNWDTLKRWEDMTMEERRETFGYKYEPKSPG
                     GSNGIAANPSANAEAARSPA"
BASE COUNT          464 a         1033 c         1020 g          430 t
ORIGIN      
        1 caggctctgc gcgcagacgt aggaatcgcc gaacatccaa agccagccag cggtcacgat
       61 gaagctgccc gggggaagaa actcgaagaa gtcccgatag atcgtgtgcc ccagcagcat
      121 ccgctggacg ccgtcgaaca gcagcccttc gtcgcccgac agcgtcgcga agtgcaggaa
      181 cggcaggcac agcgcgccgg tgacacacgc caccatcagc agccagcacc aggcaagccg
      241 gtcctccttc gcccgggcca gcatgcgcac gacaccggac gatgagtcag gcctggccag
      301 agcatctgct ccgaacacct tggtgtctcc atgcgggtga gcggctgcga ccggcgttcc
      361 ccctaatata agggctgtgc cgggcgcaaa tgtttcttcc gcctccggtg tgatcgcgca
      421 cgcggcgccc ttgacgcacg ccgccgcaag ctgccggaat ggctgcacat cacgcaggac
      481 cacgccgaca tgtcagacgc cgtcatcgcc agcctcgccg agcagcgcaa cgccatcctg
      541 gaccggctga aggcgctgct gcggattccc agcgtctcca ccgatcccgc cttcgcgccg
      601 cacatgcagg ccgcccgcaa cttcctgctc cagcgcctgc gcgacggcgg cctgcacaac
      661 gtccagctgc ttgatggcgg cggccagccc gccgtgttcg gcgagtggct cggcgcgccc
      721 ggcaagccga cgctgatcgt ctacggccat tacgacgtgc agccggcgga cccgctggag
      781 ctgtgggact cgcccccctt cgaacccacc gaacgcaccg gccgcctgcc ggtcaacatc
      841 aagctgttca tcgagggcga ggaggaaagc ggcagcccca gcgtgcgcgc catcgtggaa
      901 cgctaccgcc atctgctgga cgccgacgcc atgctgtccg ccgatggcgg gcgcgccagc
      961 accaccatcc cgaccctcgg catcggcgga cgcggcatca ccgggctgca gttcaccctg
     1021 cgcaccgcga agaaggacgc ccattccggc cgctatggcg gtgccgcacg caacgcgctg
     1081 cacgacatgg cggcgctgat cgccagcctg cacgacgcgc agggccgcat cctggtgccg
     1141 cattacggcg acgacgccgc gccgatcacc aactccgccc gcgccgccgc cgccgcgctg
     1201 ccgtccgacg acgcagcgtt cctggccgcc atcggcggcg tgcgttacgg cgacccgaac
     1261 tacaccgtgc gcgagctgac cacgctgtgc ccgaccatcg aggtgaacgg catgtggggc
     1321 ggctataccg gcgccggcgg caagaccgtg ctgcccgccg aggcgcacgc caagatcacc
     1381 atgcgcctgg cgcccggcat ggaccccgcg cgcgcccgcc agcacctgga ggcgcatctg
     1441 cgggcgcagg tgccgcacgg ggtggcgctg cagatcagcg cggagaaggg cggcaccccg
     1501 gcgccgacgc taccggacga ccacccgctg ctggtcaccg cctcgcgcgt gcaggagcaa
     1561 ctgcatggcc gcaagccgat cccggtgcgc gccggcggca ccatcccgat caccgcggtg
     1621 ttccgcgaga tgctgggcat cgacacgctg acctacggcc tggcgatgcc ggacgaggac
     1681 gtgcacgcgc cgaacgagtt cttccggctg tcgtccttcg atgaggggtt gaagagctgg
     1741 ccgatgctgc tgaccgcttt gggcgatctg agcgcggcgg attttgcgcg gttccgccac
     1801 tgacggcgcg cgccggccgc gcggagttgg ggttccccgc ggcagggcgc gcatgcgcat
     1861 cccggtgtca tggtccgcga aggccgcccg cgcgtcatgg gcagcgaggg cgtgcccgcg
     1921 cgtcaggggc cgcgacggcg cacccccgcg tcatggtccg cgaagtcgag cctccgtgtc
     1981 atggtccgcg aaggcggacc atccacgact ggctggcatg gttcagaaca aagcagagcg
     2041 tcaggctggg ctgcgggcag cctccgcgtt ggcggatggg ttcgcggcaa tcccgttgga
     2101 tccaccgggc gatttcggct cgtatttgta gccgaatgtc tcccgccgtt cctccatcgt
     2161 catgtcttcc cagcgcttca gcgtgtccca gttctgcgcc agtcgcgcga gcgagggatc
     2221 cttggcgtag gcctcggcca ccgcctgcgc caccgggaag cctgcggcgg tggggcggtt
     2281 cgccggggcc ggcattgcgg cgacaggggg attttcctgg cccgccgccc tgccgcgcgg
     2341 tgggttggct aacctgaccg gcaccgtggg gcgcggctcg ccgccgtccg cgtcctgcag
     2401 catcggccgt gttctactgc ggtggagagc ccgctgcgtg gcgatggcgg aacgctgcat
     2461 cgccagcccg tcgcgccgta aacgggaggc ttccttgctc acagggtcga gtccttccag
     2521 cgcatcgaaa cacgcgttgg cctggtgcca cgcggcgagg aattgcgagg cgagcatcgc
     2581 ctcctccagg tcgcgcggct gcatctcatt catctgctgg atcagcaggg ccttgcgctg
     2641 ctccggggtg gctgcgccgg gtatgaccgg cacggcggcg agcaggccgc tgagcaggct
     2701 ggcggagagt tccggggtga gggcgaggag gctgaggcag agatggctga gggacatgat
     2761 ggccggctcc tttctggccg gaaggaaacg tgcgaacaat agcggaactt aggaacttag
     2821 tcaaggcata ttttctggtt ggcgggaggc ggccggcggc cggtccgggg agaccgcgcg
     2881 gcggcgggcg gcttggtggt tggtcaaaga aagcaagcat tcaacacggg tgcgccggga
     2941 gaccggc
//
LOCUS       DASSZH010000158         2096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_37813, whole genome shotgun sequence.
ACCESSION   DASSZH010000158 DASSZH010000000
VERSION     DASSZH010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37813"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VFN42_03425"
     CDS             complement(<1..347)
                     /locus_tag="VFN42_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995695.1"
                     /translation="MSRRQDRPRRAHPRPHRRAGLPETLVLQITGTDPDGDAIARPAS
                     WDNAAGPPPLIYMAPEPRGRPALAPGERVLARLRPIPGAGPPRYEGRTMKRLTTGPAR
                     ILGVFRPGQPQSR"
     gene            657..791
                     /locus_tag="VFN42_03430"
     CDS             657..791
                     /locus_tag="VFN42_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995696.1"
                     /translation="MNTTPFDFDVISGPSVPRDDRQPEPPQGDAQPVAGTAPAQPQTR
                     "
     gene            843..>2096
                     /locus_tag="VFN42_03435"
     CDS             843..>2096
                     /locus_tag="VFN42_03435"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HET8995697.1"
                     /translation="MPAYPDPAVLAAAESELRALPPLIFAGEARALQAQLADVAAGRG
                     FLLLGGDCAESFAEFHANAVRDTVKVLLQMAVVLSYAAAAPVVKVARMAGQFAKPRSV
                     PAETVDGVTLPSYRGDIINDAAFTAAARLPDPQRMLRAYHQAAVTLNLLRAFAQGGFA
                     DLRRVQQWNQAFVAASPQGTRYAELARRIEESLAFMAACGLQSAAVPALRSTELFTAH
                     DALLLPYEEALTRQDSLTGDWYDCSAHLLWIGDRTRQVDHAHVAFLRGVANPLGLKCG
                     PSLEPDDLLRLLDALNPGNVPGRMTLITRMGAETLGSNLPGLIRAVRREGRHVVWCCD
                     PMHGNTVTLADGRKTRPFAQILREVNAFFAIHAEEGSHPGGVHLEMTGKDVTECLGGD
                     QQIEEADLAARYQTHCDPRLNGTQAL"
BASE COUNT          313 a          700 c          768 g          315 t
ORIGIN      
        1 atgcgggatt gcggctggcc gggacggaac acgcccagga tgcgggcggg accggtggtc
       61 agccgcttca tggtgcggcc ctcgtagcgc ggcggccccg caccggggat cggtcgcagc
      121 cgcgcgagca cgcgttcgcc aggcgccagc gcggggcggc cgcgcggttc gggcgccatg
      181 tagatcagtg gtggcgggcc ggcggcgttg tcccagcttg ccgggcgcgc gatcgcatcg
      241 ccgtccgggt cggtgccggt gatctgcagc accagcgtct ccggcaatcc ggcacggcgg
      301 tgcgggcgcg gatgggcgcg gcgcggacgg tcctggcggc gggacaaggc aactccatgc
      361 gggtccgggg tatgcggata cggaatatag gcggtggcgc gcggcttgtc agacgccggg
      421 agcaccgtcg cgcttgacgt gcgcgccggc ctcgggctat gcgccatgca tggcatcgat
      481 ccgacgcccg accgtgcgat tttggtaccg ctggtacatt ccggcggcct aggatcgtgc
      541 acagctaaag caagaccccg aagccgcctc accaaggcgg ctttcgtatt tctggacttg
      601 cctgcacgaa attcccgcca gcggtggagc ggcctcccaa cccgaaggag gctgacatga
      661 acaccacccc gttcgatttc gacgtgatct ccggcccgtc cgtgccgcgc gacgaccggc
      721 agccggagcc gccgcagggc gacgcgcagc cggttgccgg cacggcgccg gcccagccgc
      781 aaacccggta gcggtccgtg tcctggaccc cggcggattg gcgcgacagg cccgcgcgcc
      841 agatgcccgc ctatccggac cccgccgtgt tggcggcggc ggagagcgaa ttgcgcgcct
      901 tgccgccgct gatcttcgcc ggtgaagccc gcgccttgca ggcccagctc gccgacgtcg
      961 ccgccggccg cggcttcctg ctgctcggcg gcgattgcgc ggagagtttt gccgagttcc
     1021 acgccaacgc ggtacgcgac acggtgaagg tgctgctgca gatggcggtg gtgctgagct
     1081 acgccgccgc cgcaccggtg gtgaaagtgg cgcgcatggc cgggcagttt gccaagccgc
     1141 gctccgtccc ggcggagacc gtggatggcg tgacgctacc cagctaccgt ggcgacatca
     1201 tcaacgacgc cgcgttcacc gccgcggcgc ggctgccgga tccgcaacgc atgctgcgcg
     1261 cataccacca ggcggccgtc acgctgaatc tgctgcgcgc cttcgcccag ggcggattcg
     1321 cggacctgcg tcgggtgcag caatggaatc aggccttcgt cgccgcgtca ccgcaaggca
     1381 cgcgttatgc cgagctggcg cggcgcatcg aggaatcgct cgcgttcatg gcggcgtgcg
     1441 gcttgcagag cgcggcggtg cccgcgctgc gcagcacgga gctgttcacc gcacacgatg
     1501 cgctgctgct gccctatgag gaagcgctga cacgacagga cagcctgacc ggggattggt
     1561 acgactgctc cgcgcatctg ctgtggatcg gtgatcgcac gcggcaggtg gaccatgcgc
     1621 atgtcgcgtt tctgcgcggg gtcgccaatc cgctcgggct gaaatgcgga ccgagcctgg
     1681 agccggatga tctgctgcga ctgctggatg cgctgaatcc gggcaatgtc ccgggacgta
     1741 tgacgctgat cacccgcatg ggggcggaaa cgctgggcag caacctgccg gggctgatcc
     1801 gcgcggtgcg gcgggagggg cggcacgtgg tgtggtgctg cgatccgatg cacggcaaca
     1861 cggtgactct ggccgatggc cgcaagacgc ggcccttcgc gcagattctg cgcgaggtga
     1921 acgcattctt cgccatccac gcggaggagg gcagccatcc cggcggcgtg cacctggaga
     1981 tgaccggcaa ggatgtcacc gaatgcctgg gtggcgacca gcagatcgag gaagccgacc
     2041 tggccgcgcg ctaccagacg cattgcgatc cgcggctgaa tggcacgcag gcgctg
//
LOCUS       DASSZH010000159         5616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_549262, whole genome shotgun sequence.
ACCESSION   DASSZH010000159 DASSZH010000000
VERSION     DASSZH010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549262"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1614)
                     /locus_tag="VFN42_03440"
     CDS             complement(<1..1614)
                     /locus_tag="VFN42_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HET8995698.1"
                     /translation="MPEGSALYAAAADATIASAGKLADPAANQPGDPMESFDYIVTGA
                     GSAGCVLANRLSESGRHRVLLLEAGGKDSNPWIHIPLGYAKTFVDPRVNWRFESEPEA
                     NLNNRAMYQPRGKVLGGTSSINGMIYMRGNHADYDEWRQRGCEGWDWDSVLPYFRKAE
                     DNQRGAGEYHGAGGPLHVADQPYEWEIGRLLLAASQQAGIPFNPDFNGAQQEGCGWYQ
                     TTTKDKRRWSTAVAYLRPARTRPNLVVRTHAHATRVLLEGGRAAGVEFRTPDGLETAL
                     ASGEVIVSGGAYGSPQLLLLSGIGPGAHLQEMGIPVLHDLPGVGFNLHDHFNTYCSWR
                     ISRPLSLNQLQHSIPRQIVAAFQYALYRGGPMSGNGMYLGAFVRSDPRLERPDLQLNM
                     TSWSTIDRTRDRIISHPHPGFAISPVHLRPEGRGTVRLKSPDPLAPPEIRFNFLRSEY
                     DYQAIVTGMRVARDIARQPALQGLVMEETLPGPQVQSEEELREDVRRRGVSNLHPVGS
                     CGMGHGPMAVVDPRLRVHGVERLRVVDASV"
     gene            1677..3536
                     /locus_tag="VFN42_03445"
     CDS             1677..3536
                     /locus_tag="VFN42_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HET8995699.1"
                     /translation="MPTRSAASSRTTARSRTAAPARRGPSPRAVPPAAGKPPPRQPDP
                     PKPSRRRWVRLLRWGFVGAVWGALALAVLVLWFAWDLPNPASALDAVRRPGLTLEDRT
                     GRIFAGYGDVVGTPMHLSQLPAYVPEAVVAVEDRRFWQFPAIDPIGILRAAWVDLTSG
                     HVVQGGSTLTQQVAKTLFLSNARTVRRKVQELLLTLWLNSSFTKRQILEIYLNRVYLG
                     AGCWGIDAASRVYFGVPAPRLKLWQAAVLAGLPRAPSRFNPRVNPEAAKARAEQVLRA
                     MVETGKISAAQAQQAAAQIAFPPPRARAAGWFADWAAEQAEAGLPPNRDATLRTTLDP
                     RLQSVAESQLQALLAGPGAKDRVGEGAVAVLDAATGAVRALVGGRDYSAGSYNRAVLA
                     RRQPGSSFKVFTFLAALQHGMRPNDTVLDAPIRIGRWAPHDFEPHYRGPVTLTEALAD
                     SLNTAAVRLEVANGGPRGVAAEARRLGITDRLPLDDSLTLGTGGVGLLEMSAAYATFF
                     NGGRKVTPWAIDGLPHPSPRPAITPEQAAMMAGMLRAVVTYGTGRAAAVPGHAVAGKT
                     GTTQDFRDAWFIGCIDGTVIGVWMGNDDNRPMKGVAGGDLPARLFHAIALAVR"
     gene            3680..4396
                     /locus_tag="VFN42_03450"
     CDS             3680..4396
                     /locus_tag="VFN42_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HET8995700.1"
                     /translation="MIQARTFGALLALGALTALPGCSMLGGGMGGGQSSQAATSQYTA
                     PAATRTAEARQQAPVEQPITKRMIRRVQANLKHDGLYRGRIDGVWGPKSEHAAREFQE
                     HNHMNPTGKIDLPMLQAMNLGSGNEQYGEANGGMGQNGNAGPNGSQQYGEANTGPNNG
                     QDSNGKISPSRNFTTGGQTNAGTAHASNMPYQPNNTAPNAAGGAAGNGANGPSGNTGT
                     MNNGMDNGAAPASGTTQPQH"
     gene            complement(4439..5500)
                     /locus_tag="VFN42_03455"
     CDS             complement(4439..5500)
                     /locus_tag="VFN42_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HET8995701.1"
                     /translation="MPYEPQRFDLAPIRINLYSDTQSKPTPAMKQAMLEAEVGDEQSG
                     SDPSVWALCDRSAALLGKQAAMFLPSGTMCNQVAIATHCRPGDEILAHRDAHILTSEA
                     GGPAAIAGATIHGLDGPRGQFTAETLEAAIRPASRYSPPQTLVEVEQTANRGGGTCWS
                     VDALRAVTGAAHNHGMATHMDGARLLNAAVALGVPASDLTEGCDTVWLDFTKGLGAPL
                     GAVLAGSREFIGRAWRWKQRLGGAMRQGGMNAAACLYTLDHHVDRLAEDHANARALAR
                     GMAQIPGITVEQPETNLVFFDTKGTGMTAAQFAAKLRPEGLLVSVFDTWRGRACLHLD
                     VTASQVEEAVAIMQRVLNR"
BASE COUNT          810 a         1974 c         1997 g          835 t
ORIGIN      
        1 caccgaggcg tccaccacgc gcagccgctc caccccatgc acgcgcagcc gtggatcgac
       61 caccgccatt ggtccgtgcc ccatgccgca actgcccacc ggatgcaggt tggatacgcc
      121 gcgccggcgc acgtcctcgc gcagctcttc ctcgctctgc acctgcggcc ccggcaaggt
      181 ctcctccatc accagcccct gtagcgccgg ttggcgcgcg atatcgcggg cgacgcgcat
      241 gccggtgacg atcgcctggt agtcgtattc gctgcgcagg aagttgaagc ggatctccgg
      301 cggcgccaag gggtccggcg atttcagccg cactgtaccg cggccctccg gccgcagatg
      361 caccgggctg atcgcgaagc cgggatgcgg gtgggagatg atgcggtcgc gcgtgcggtc
      421 gatcgtgctc caggacgtca tgttcaactg caagtcgggc cgttccagcc ggggatcgct
      481 gcgcacgaag gcgcccagat acattccgtt cccggacatt ggcccgccgc gatacagcgc
      541 gtactggaac gcggcgacga tctgccgcgg aatgctgtgc tgcagctggt tgagcgacag
      601 cggccgcgat atccgccagg agcagtaggt gttgaagtgg tcgtgcaggt tgaagccgac
      661 gcccggcaga tcgtgcagca ccgggatccc catctcctgc agatgcgccc ccggaccgat
      721 gccggacagc agcagcagtt gcggcgagcc gtaggcaccg ccggagacga tcacctcgcc
      781 ggaggccaaa gcggtttcca gcccgtccgg ggtgcggaac tccaccccgg cggcgcggcc
      841 gccttccagc agcacccggg tggcatgggc atgggtgcgg accaccaggt tcggccgggt
      901 gcgcgccggg cgcagatagg ccaccgccgt gctccagcgg cgcttgtcct tggtggtggt
      961 ctggtaccag ccgcagcctt cctgctgcgc accgttgaag tcggggttga acgggatgcc
     1021 ggcctgctgc gaggcggcga gcagcagccg gccgatctcc cactcatagg gctggtcggc
     1081 gacgtgcagc ggcccgccgg cgccgtggta ttcgccggcg ccgcgctggt tgtcctcggc
     1141 cttgcggaaa tagggcagca cggagtccca gtcccagcct tcgcagccgc gctgccgcca
     1201 ttcgtcgtag tcggcgtgat tgccgcgcat gtagatcatg ccgttgatcg agctggtgcc
     1261 gcccagcacc ttgccgcgcg gctggtacat ggcccgattg ttgaggtttg cctccggctc
     1321 gctctcgaac cgccaattga cgcggggatc gacgaaggtc ttggcgtagc ccagggggat
     1381 gtggatccag ggattgctgt ccttgccgcc ggcttccagc agcagcacgc ggtggcggcc
     1441 ggactcgctc agccggttgg ccaggacgca gccggccgac ccggcgccgg tgacgatgta
     1501 gtcgaagctt tccattgggt cccccggttg attggccgca ggatcggcga gcttgccggc
     1561 ggaggcaatc gtcgcgtccg ccgccgcggc gtacagggcg cttccctccg gcaccccggc
     1621 cgggccagac tccaggaccc gctggcgcgc cggtggggat cttgcccgag tcccggatgc
     1681 ccacgcgttc tgctgccagt tcccgcacca ccgcccgatc ccgcaccgcc gcgccggcgc
     1741 ggcgcggccc ctcgccgcgc gcggtgccgc ccgccgccgg caagccgccg ccgcgccagc
     1801 ccgatccgcc gaagccgtcc cgccggcgct gggtccgcct gctccgctgg ggcttcgtcg
     1861 gcgccgtctg gggggcgctg gcgcttgccg tgctggtcct gtggttcgcc tgggacctgc
     1921 ccaacccggc ttccgcactg gatgcggtgc gccgtcccgg cctgacgctg gaggaccgca
     1981 ccgggcgcat cttcgccggc tatggcgacg tggtgggcac ccccatgcac ctgtcgcagc
     2041 ttccggccta tgtgccggag gcggtggtcg cggtggagga ccggcgcttc tggcagttcc
     2101 ccgccatcga cccgatcggc atcctgcgcg ccgcctgggt cgatctgacg tccggccacg
     2161 tggtgcaagg cggctccaca ctcacccagc aggtcgccaa gacgctgttc ctcagcaacg
     2221 cccggacggt tcggcgcaag gtgcaggaac ttctgctaac actctggttg aatagctctt
     2281 tcaccaagcg gcagatcctg gagatctacc tgaaccgggt ctatctgggc gccggctgct
     2341 ggggcatcga cgcggcgtcg cgggtgtatt tcggcgtgcc cgcgccgcgg ctgaaactgt
     2401 ggcaggccgc ggtgttggcg gggttgccgc gcgcaccgtc ccgcttcaac ccgcgggtga
     2461 acccggaggc cgccaaggca cgggccgagc aggtgctgcg cgcgatggtg gagaccggga
     2521 agatcagcgc ggcgcaggca cagcaggcgg ccgcgcagat cgccttcccg ccgccgcgcg
     2581 cccgcgctgc cggctggttc gccgactggg ccgcggagca ggcggaggcc ggcctgccgc
     2641 ccaaccgcga cgccaccttg cgcaccacgc tcgacccgcg gcttcaaagc gtggcggaga
     2701 gccaattgca ggcgctgctt gccggcccgg gcgcgaagga tcgcgtcggc gagggtgccg
     2761 tcgcggtgct ggacgccgcc accggcgcgg tgcgcgccct ggtcggcggg cgtgactaca
     2821 gcgccggatc ctacaaccgc gccgtgctcg ctcggcggca gccgggatcg tcgttcaagg
     2881 tgttcacctt cctggccgcc ctgcaacacg gcatgcggcc gaacgatacg gtgctggacg
     2941 cgccgatccg catcggccgc tgggcgccgc atgatttcga gccgcattac cgcggtccgg
     3001 tgacgctgac cgaggcgctg gccgattcgc tgaacaccgc cgcggtgcgc ctggaagtgg
     3061 cgaatggcgg cccgcgcggc gtcgcggcgg aagcgcggcg gctgggcatc accgaccggc
     3121 tgccgctgga cgattccctg accctcggca ccggtggcgt tggtctgctt gaaatgtcag
     3181 cagcctacgc gacgtttttc aatggtggtc ggaaggtcac gccctgggcg attgacgggc
     3241 tgccgcatcc ctcgccgcgg ccggcgatca ccccggagca ggcggcgatg atggccggca
     3301 tgctgcgggc ggtggtgacc tacggcaccg ggcgcgcggc ggcggtgccg ggccatgcgg
     3361 tggcgggcaa gaccggcacc acccaggatt tccgcgatgc ctggttcatc ggctgcatcg
     3421 acggcacggt gatcggcgtc tggatgggca acgacgacaa ccggccgatg aagggcgtgg
     3481 ccggcggcga cctgccggcc cggttgttcc atgccatcgc cctggcggtc cgctgaggcc
     3541 gtccggcttc aaataccatt gctcctggca accggatgcg gcgcgatccc tttcgggtcc
     3601 atgcctgagc ctacctgccg ttccacctgc gggcggcagc ctgccggctc gtccggttca
     3661 ccctgacagg aggaatgcaa tgatccaggc tcgcaccttt ggcgccctgc tcgcactcgg
     3721 cgcactgacc gcgctgcccg gctgctcgat gctgggcggc ggcatgggcg gcggacagtc
     3781 cagccaggcc gccacctcgc aatacaccgc gccggcagcc acccgcaccg ccgaggcccg
     3841 tcagcaggcc ccggtcgaac agccgatcac caagaggatg atccgcaggg tgcaggccaa
     3901 cctgaagcat gacggcctgt atcgcggccg tatcgacggc gtctggggcc cgaagagtga
     3961 gcacgccgcg cgggagttcc aggagcacaa ccatatgaac ccgaccggga agatcgacct
     4021 gccaatgctg caggcgatga acctgggcag cggcaacgag cagtacggtg aggccaatgg
     4081 cggcatgggg cagaacggca atgccggccc gaatggcagc cagcagtacg gcgaggcgaa
     4141 taccggcccg aacaacggcc aggattcgaa cggcaagatc agcccgagcc ggaacttcac
     4201 caccggtggg cagaccaatg ccggcacggc gcatgccagc aacatgccgt accagccgaa
     4261 caacaccgcg cccaacgccg ccggtggcgc ggccgggaat ggggccaatg gcccgagcgg
     4321 caacaccggc accatgaaca acggtatgga caacggcgcg gcgccggcca gcggcacgac
     4381 ccagccgcaa cactgacctg ggccggggcc ggcgggagaa gtcccagccg gctcccgcct
     4441 agcggttcag cacacgctgc atgatggcta ccgcctcctc cacctgggag gcggtgacgt
     4501 ccagatgcag gcaagcgcgc ccgcgccagg tatcgaacac cgacaccagc aggccttccg
     4561 gccgcagctt cgcggcgaac tgcgccgcgg tcatcccggt gcccttggtg tcgaagaaca
     4621 ccaggttggt ttccggctgt tccaccgtga tgccgggaat ctgtgccatg ccgcgggcca
     4681 gggcgcgggc gttggcgtgg tcctcggcca gccggtccac atggtggtcc agcgtataca
     4741 ggcaggccgc ggcgttcatg ccgccctgcc gcatcgcccc gcccagccgc tgcttccagc
     4801 gccacgctcg gccgatgaac tcccgcgatc cggccagcac cgcgccgagc ggggcgccga
     4861 ggcccttggt gaagtccagc cagacggtgt cgcagccttc ggtcaggtcg ctggccggca
     4921 cgcccagcgc caccgcggca ttcagcagcc gggctccgtc catgtgggtg gccatgccgt
     4981 ggttgtgcgc cgcgccggtc accgcgcgca gcgcgtccac gctccagcag gtgccgccgc
     5041 cgcggttggc ggtctgctcc acctcgacca gggtctgcgg cggcgaatag cgggaggccg
     5101 gacggatcgc cgcttccagc gtctcggcgg tgaactggcc gcgcgggccg tccaggccgt
     5161 ggatcgtcgc gccggcgatg gccgccgggc cgccggcttc gctggtgagg atgtgggcat
     5221 cgcgatgcgc caggatctcg tcgccggggc ggcaatgggt ggcgatggcc acctggttgc
     5281 acatggtgcc ggagggcagg aacatcgccg cctgcttgcc gagcagcgcg gcgctgcggt
     5341 cgcacagcgc ccagacgctg ggatcggagc cggactgctc gtcgcccacc tcggcttcca
     5401 gcatcgcctg cttcatcgcc ggggtcggct tggactgggt gtccgagtag aggttgatgc
     5461 ggatgggggc gagatcgaag cgctgcggtt cgtagggcat ggcgggcacc gtcggttgaa
     5521 gcgtctgcgc gtcccgtgga ctggcggggg cggcctgcga tccggatatg ggaaacgccg
     5581 gacgcggtcc agcccgagcg gctgtgtcgg tgcgaa
//
LOCUS       DASSZH010000160         2849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1094965, whole genome shotgun sequence.
ACCESSION   DASSZH010000160 DASSZH010000000
VERSION     DASSZH010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094965"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1390
                     /locus_tag="VFN42_03460"
     CDS             <1..1390
                     /locus_tag="VFN42_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET8995702.1"
                     /translation="GESDLDESPITGESIPVLRREGDTVVAGSINASAALQVRVTRAA
                     EDNTVARIIRMVEDAQASKAPIARFIERFSRVYTPAAVGIAVMVAVLPPLAFGQPWES
                     WIYRGLALLLIACPCALVLSTPAAIASGLAVGARRGLLVKGGAALEAIARVNTAAFDK
                     TGTLTEGRPRVTDLFAVAGTERSLVGLAAAVENGSSHPLARAILARAAADGIPLRPAR
                     QARAIPGRAAEAVVTGKRVAVGSPVYAAEQAPLATELAERIAVWEGQGKTVVAVLVDG
                     APVGLIALRDEPRSDAAAGIRQLRALGVQPVMLSGDNRRTAEAVAARVGLHAVAELLP
                     QDKVREIGALRQRGPVLMVGDGINDAPSLATADVGIAMGGGTDVALETGDAAILHDRV
                     ADVAALVRLARATLANIRQNVTVALGLKGVFLVTTIAGFTGLWPAVMADTGATVLVTL
                     NALRLLRVRCGE"
     gene            complement(1407..2450)
                     /locus_tag="VFN42_03465"
     CDS             complement(1407..2450)
                     /locus_tag="VFN42_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010667673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET8995703.1"
                     /translation="MLAMISAALFGASTPVAKFLLGQINPWLLAGVLYLGSGIGLAAV
                     HIGRRSCAIAPPEAPLRRADLPWLAAAVLAGGVIGPLLLMLGLSVTPASSAALLLNLE
                     GLATMAIAWLVFREHTDRRIVLGALLILFGAVLLSWQGEVAGVGWGALAIVGACLAWG
                     ADNNLTRKLSAADPVQIAMIKGLAAGAVNTVLALWFGAHAPSAGTLIGGAAVGFLGYG
                     VSLVLFVLGLRHLGSARTAAYFSTAPFIGAVLALVMFREPPTATLLAAAVLMGAGLWL
                     HLAEHHEHQHVHEEMVHEHVHVHDEHHQHAHGPDDPPGEPHVHVHRHQRLAHRHAHYP
                     DLHHQHTHAAPAD"
BASE COUNT          431 a          962 c         1040 g          416 t
ORIGIN      
        1 cggcgagtcg gacctggatg aaagcccgat caccggcgaa tccatcccgg tgctccgccg
       61 cgagggcgac accgtggtgg ccggcagcat caatgcctcc gccgcattgc aggttcgggt
      121 gacgcgcgcc gcggaagaca acaccgtggc gcgcatcatc cgcatggtgg aggacgcgca
      181 ggccagcaag gcgccgatcg cccggttcat cgagcggttc agccgcgtct atacgccggc
      241 cgcagtcggt attgcggtga tggtggcggt gctaccgccg ctcgccttcg gccagccgtg
      301 ggaaagctgg atctatcgcg gactggcgct tctgctgatc gcctgtccct gcgcgctggt
      361 gctctccacg ccggcggcga tcgcctccgg cctggcggtg ggtgcgcggc gcgggctgct
      421 ggtgaagggc ggcgcggcgc tggaggcgat tgcccgggtc aacaccgccg cgttcgacaa
      481 gaccggcacg ctgaccgagg gccggccgcg cgtcaccgac ctgttcgcag tcgccggcac
      541 cgagcgttcc ctggtcggcc tggccgccgc ggtggagaat gggtccagcc atccgctggc
      601 gcgggcgatc ctcgcccggg ctgcggcgga cggcatcccg ctgcgccccg cccgccaggc
      661 gcgcgccatc cccggccggg ccgccgaggc ggtggtcacc ggcaagcggg tcgccgtcgg
      721 ctcgccggtc tatgcggcag agcaggcgcc gctggcgacg gaactggcgg agcgcatcgc
      781 ggtgtgggaa ggccagggca agacggttgt tgcggtgctg gtggacgggg cgccggtcgg
      841 gctgatcgct ctgcgcgacg agccccgcag cgatgccgcc gccgggatcc ggcagttgcg
      901 cgcactgggc gtgcagccgg tgatgctgtc cggcgacaac cggcgcaccg ccgaggcggt
      961 cgccgcccgc gtcgggctac acgccgtcgc cgagttgctg ccgcaggaca aggtgcggga
     1021 gatcggcgcc cttcgccagc gcgggccggt cctcatggtc ggcgatggga tcaacgacgc
     1081 gccgtcgctg gcgaccgcgg atgtcggcat cgccatgggt ggcggcaccg acgtggcgct
     1141 ggaaaccggc gatgccgcga tcctgcacga ccgggtggcg gacgtggcgg cgctggtgcg
     1201 gctcgcccgg gcgacactcg ccaatatccg ccagaacgtg acggtggcgc tggggctgaa
     1261 aggcgtgttc ctggtgacca ccatcgccgg gttcaccggc ctgtggcccg cggtgatggc
     1321 agacaccggc gccaccgtgc tggtcacgct gaacgcgctg cggctgctgc gggtgcgctg
     1381 cggcgaatag cgcgcagcca cctgcttcaa tcggcgggcg ctgcgtgcgt gtgctggtga
     1441 tgcaggtccg ggtagtgggc gtgccggtgc gccaatcgct gatggcgatg cacgtgcaca
     1501 tgcggttcgc ccggtgggtc gtccggtccg tgcgcatgct ggtggtgttc gtcgtgcaca
     1561 tggacgtgct cgtgcaccat ctcctcgtgc acgtgttgat gctcatgatg ttcggcgagg
     1621 tgcagccaaa gcccggcgcc catcagcacc gcggcggcga gcaatgtcgc ggtgggaggc
     1681 tcgcggaaca tgaccagcgc cagcaccgcg ccgatgaacg gcgcggtgga gaaatacgcc
     1741 gcggtcctgg ccgaaccgag atgccgcaac ccgagcacga acagcaccag gctgacgccg
     1801 tagccaagga agccgaccgc cgcgccgcca atcagcgttc cggcgctggg tgcatgcgcg
     1861 ccgaaccaca gggcgagcac cgtgttgacc gcgccggcag ccagcccctt tatcatcgcg
     1921 atctgtaccg gatcggccgc actgagcttg cgcgtcaaat tgttgtcggc accccaggcc
     1981 aggcaggccc caacaatggc cagcgcaccc cagccgacgc ccgccacctc gccttgccag
     2041 gacagcagaa ccgcgccgaa caggatcagc agtgcaccca ggacaatccg gcggtcggtg
     2101 tgttcgcgga acactagcca agcgatcgcc atcgtggcga ggccctccag gttcagcagc
     2161 agggcggcgc tggaagccgg cgtcacgctg aggccgagca tcagcagcaa cggaccgatg
     2221 acaccgccgg ccagcacggc ggcggcgagc cacggcaggt cggcgcggcg cagcggcgcc
     2281 tcgggcggcg cgatagcgca cgaacggcgg ccgatatgca cagccgccag accgatgccg
     2341 gagcccaggt aaagcactcc ggccagcagc cagggattga tctgccccag cagaaatttc
     2401 gcgaccggcg tactggcgcc gaacagggcg gcggagatca tggccagcat ggcgcctggg
     2461 gatgcgaggg cggggatggg caatttggtt ccctacgacg gtggcgaccg atttgtattg
     2521 cagctcgggc agcacggcta ttcagctggc ggttgccttc ggtttcgcgg cagtgtggat
     2581 gctctatgtg tcagtgcggc tgatctgaca gttttgcgat caacgcgcag tcctgtcgct
     2641 gcttcggagc gcactgttgg accgcccccg caaggatgcg ctcccgcaca gggcgtgccg
     2701 gccaacggcg aactgtcctg catcgtcact caactgcagg gcgtggcgca gcaaaccgtg
     2761 cgcattgatg ctgccccaca gacccccgag gcgatccgcg cggcgtctgg aatgaccgcc
     2821 aggcccggct ttgccggtgc ggctatgcc
//
LOCUS       DASSZH010000161         3282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_515142, whole genome shotgun sequence.
ACCESSION   DASSZH010000161 DASSZH010000000
VERSION     DASSZH010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515142"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="VFN42_03470"
     CDS             complement(<1..1073)
                     /locus_tag="VFN42_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HET8995704.1"
                     /translation="MSATQDTLRAALREIKDPASGRDVVSAGLVESIQQNGGLVQVTL
                     LTDRAHAAAMEPVRRAAEQALARVPGVTNATAILTAHKPAGPAAAPPPRAAGHAHPPA
                     QGAAGQKASLLLPEVKAIVAVASGKGGVGKSTVAVNLAVSLARAGHRVGLLDADIYGP
                     SLPRMVGLNRKPQVQGEKMMPLHVWGISCMSIGFLVDEETPMIWRGPMVMGALEQMMG
                     QVAWGALDVLVVDMPPGTGDAQLTMAQRVALTGAVIVSTPQDIALIDARRGVRMFEKT
                     QVPVLGLVENMSFFTCPHCGGRSDIFGHGGAKLEAERLGAEFLGEIPLLLDIRTASDA
                     GTPIAAAAPDSEGAKAYAALAERV"
     gene            1428..2528
                     /gene="hflK"
                     /locus_tag="VFN42_03475"
     CDS             1428..2528
                     /gene="hflK"
                     /locus_tag="VFN42_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HET8995705.1"
                     /translation="MIARLQAAVRRLMGGGGGPRGRFPNGRGLLLLGLGAVALWLASG
                     LYRVQPDELGVVLRFGAYEYRTQPGLNWHLPWPIESVELPAVTRINRTEIGYRSSARN
                     QSTVDVPAESLMLTGDENIIDINVTIFWKIDNAADYLFNVRDPDALVKAVAESSLREV
                     IGRTPIQPALTQARAQIETDVKTQTQQIMNAYKAGVDITQVQLQRVDPPAGVIESFRD
                     VQRANTDAERMRNEAEAYRNDIIPRARGDAARIIAEAQGEKLAAIAQAKGQAQRFDSV
                     LKAYEAAKDITLKRMYLDTMNDVLTHARALVVDDKLKGLVPFLPLSTPPANPAAPPPS
                     PAPGSGAAAKPMEQSMQSQPGSQLLQPTDSGR"
     gene            2525..>3282
                     /locus_tag="VFN42_03480"
     CDS             2525..>3282
                     /locus_tag="VFN42_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HET8995706.1"
                     /translation="MNRALIATIAACVVLLVLADSCLYTVSQTQQALVTQFGRVVRVV
                     QTPGLHVKTPFVQSVLIFDKRLQAVEQPGQEVILGDQRRLIVDSFAVFRITNPLLYFQ
                     AVGTAEGIRGRLNSVVSGSLRRVLGDNKLLDVLSADRDRIMRRIRDEVNREMSGFGIA
                     VADVRIRRADLPEENTQAILQRMQSERQRVAKLARAEGAEASLKIRADAERDRTVLVA
                     EARATADKLRGEGEAEAIRISAKAYNQDVKFYSA"
BASE COUNT          543 a         1166 c         1095 g          478 t
ORIGIN      
        1 acccgctccg ccagcgccgc ataggccttc gccccctcgc tgtccggtgc cgccgccgcg
       61 atcggcgtac ccgcgtcgga cgcggtgcgg atatccagca gcagcgggat ctcgccgagg
      121 aattcggcgc ccagccgctc cgcctccagc ttcgcgccgc catggccgaa aatgtcggag
      181 cggccgccgc aatgcgggca ggtgaagaag ctcatgttct ccaccaggcc cagcaccggc
      241 acctgggtct tctcgaacat ccgcacgccg cgccgcgcgt cgatcagcgc aatgtcctgc
      301 ggggtggaaa cgatcaccgc gccggtcagc gccacgcgct gcgccatggt gagctgcgcg
      361 tcgccggtgc ccggcggcat gtccaccacc agcacgtcca gggcgcccca ggccacctgg
      421 cccatcatct gctccagcgc gcccatcacc atcgggccgc gccagatcat cggagtttcc
      481 tcatcgacca ggaagccgat ggacatgcag gagatgcccc agacatgcag cggcatcatc
      541 ttctcgccct gcacctgcgg cttgcggttc agcccgacca tgcgcggcag gctggggccg
      601 tagatgtcgg cgtccagcaa tccgacccgg tggccggcgc gcgccagcga caccgccagg
      661 ttcaccgcca cggttgactt gccgacgccg cccttgccgg aggccacggc gacgatcgcc
      721 ttcacctcgg gcagcagcag tgacgctttc tggccggccg ccccctgcgc cggtggatgc
      781 gcgtgccccg cagcgcgggg cggcggagcg gcagcgggtc cggccggctt gtgcgcggtc
      841 agtatcgcgg tggcgttggt cactccgggc acccgggcca gcgcctgttc cgccgcccgc
      901 cgcaccggct ccattgccgc cgcatgcgcc cggtcggtca gcagcgtcac ctgcaccaga
      961 ccgccgttct gctggatcga ttccaccagg ccggcactga ccacgtcgcg ccccgaggcc
     1021 ggatccttga tctcccgcag cgccgcgcgc agcgtgtcct gagttgcgct catccgtcga
     1081 tcacacctag ttgttgcaaa cgcccggcca gcggccgata agcagcccgc cgtgacgccc
     1141 atgccagaag gatatggcgc agggccattg tttccgccag cggccccagg ccgcacggcc
     1201 tgccaggagg cttcccctcg tccatgtcat ggaacagtgc cggccccgat gacaacccca
     1261 acccctgggg ctcatccggt ggcccaggcc agggcggcca gcgcccgccg ccgggtggcg
     1321 gcggtggacc ctggggtagt ggcggcggac cgcagcggcc cggcggcgga ccgggtccag
     1381 gctccggacg tcccggcggc cgcggcccca ttccggacct cgatctgttg atcgcccggc
     1441 tccaggcagc cgtgcgccgc ctcatgggcg gtggcggcgg accacgcggc cgcttcccca
     1501 acggccgcgg gttgctgctg ctggggctcg gcgcggtcgc gctgtggctg gccagcgggc
     1561 tgtaccgcgt gcagccggat gaactcggcg tggtgctgcg tttcggcgcc tatgaatacc
     1621 gcacccagcc ggggctgaac tggcacctgc cctggccgat cgagagcgtg gagctgcccg
     1681 ccgtcacccg catcaaccgc accgagatcg gctaccgatc cagcgcgcgc aaccagagca
     1741 ctgtcgacgt gccggcggaa agcctgatgc tgaccggcga cgagaacatc atcgacatca
     1801 acgtgacgat cttctggaag atcgacaacg cggccgacta cctgttcaac gtgcgcgacc
     1861 ccgacgcgct ggtgaaggcg gtggcggaga gttcgctgcg cgaggtgatc ggccgcacgc
     1921 cgatccagcc cgcactgacc caggcacgcg cgcagatcga aaccgacgtg aagacccaga
     1981 cgcagcagat catgaacgcc tacaaggcag gcgtcgacat cacccaggtg cagttgcagc
     2041 gcgtcgatcc gccggccggt gtgatcgaga gtttccgcga cgtgcagcgg gccaacaccg
     2101 atgccgagcg catgcgcaac gaagccgagg cctatcgcaa cgacatcatc ccgcgcgccc
     2161 gtggcgacgc cgcccgtatc attgccgagg cgcagggcga gaagctggcc gccattgccc
     2221 aggccaaggg ccaggcacag cgcttcgaca gtgtgctgaa ggcgtatgag gcagccaagg
     2281 acatcacgct gaagcgaatg tatctcgaca ccatgaacga cgtcctgacg catgcgcgcg
     2341 cactggtggt tgacgacaag ctgaagggcc tggtgccgtt cctgccgctg agcacgccgc
     2401 cggcgaatcc ggcagcaccg ccgccatcgc ctgctcctgg ttctggtgct gcggcgaaac
     2461 ccatggagca gtcgatgcaa tcgcagcccg gttcgcaact gcttcagccg acggatagcg
     2521 gacgatgaac cgggcactga tcgcgacaat cgccgcatgc gtcgtattgc tggtactggc
     2581 ggatagctgt ctctatactg tcagccagac gcaacaggcc ctggtcacgc aattcggccg
     2641 cgtggtacgc gtggtgcaga cgcccgggct gcacgtcaag acacccttcg tgcagtcggt
     2701 actgatcttc gacaagcggc tgcaggcggt ggaacagccg ggccaggaag tgatcctggg
     2761 cgaccagcgc cgactgatcg tggacagctt tgcggtgttc cgcatcacca atccactgct
     2821 gtacttccag gcggtgggca cggcggaagg catccgtggc cgcttgaact cggtggtcag
     2881 tggcagcctg cgccgggtgc tgggcgacaa caagctgctg gacgtgcttt ccgccgaccg
     2941 tgaccgcatc atgcggcgta tccgcgacga ggtgaaccgc gagatgagcg gcttcggtat
     3001 cgcggtggcg gacgtgcgca tccgccgcgc cgacctgccg gaggaaaaca cccaggcgat
     3061 cctgcagcgc atgcagtcgg aacgtcagcg ggtggccaag ctggcccggg cggagggcgc
     3121 cgaggcgtca ctgaagatcc gcgccgacgc cgaacgcgat cgcacggtgc tggtggcgga
     3181 ggcccgcgcc accgccgaca agctgcgcgg cgagggcgag gcggaggcga tccgcatctc
     3241 cgccaaggcg tacaaccagg atgtcaaatt ctattccgcc tg
//
LOCUS       DASSZH010000162         4080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_498046, whole genome shotgun sequence.
ACCESSION   DASSZH010000162 DASSZH010000000
VERSION     DASSZH010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498046"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VFN42_03485"
     CDS             <1..313
                     /locus_tag="VFN42_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995707.1"
                     /translation="FDNPDDDLFTEEAILALARRGKIGVVIAAICRDLGLVPSVVSEQ
                     QWKDVADLITDFGGNFVALIGDAMNRVLTYIHAASFTKPLSGAEIGILNDAIFLRPAP
                     A"
     gene            complement(590..2563)
                     /locus_tag="VFN42_03490"
     CDS             complement(590..2563)
                     /locus_tag="VFN42_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET8995708.1"
                     /translation="MSGTSNGRSPRSVALVGPYSAGKSTLFEALMDAAGAPVKRPADL
                     RNRPMTTEVRVGHCTYLNDPWALLDCPGSIEFAHEACAALAMVDFAVVVCEPVPSRAL
                     TLAPLLKLLADEAIPHMIFINKIDTLDGSVRDTLAALQGLVKCPLVLRQVPIVEGQSV
                     NGYVDLISERAYRYRKGQPSELMQVPTEMQAEEQEALGKLVEVLADHDDVLLEKVLED
                     VKPTPQEIYRDLHKDLLAGSVIEVLLGSAENANGVRRLWKALRHDVPDVRETAERKGI
                     DAEGPALAQVFKTVHAGHTGKLSYARIWRGEIKDGATLGGTRLGGIYHLSGGELSKVP
                     ETQAGDVAALGRLDGVPTGATVSPAGTPEQLPFPDPPPPVYSLAIATTDHKDDVKLSG
                     ALQKLLEEDPALTMEQNQDTAETVLHGQGEMHLNNTVQRLASNYNLKVSTHKPQVAYK
                     ETIRQAVQEHGRLKRQTGGHGQFADVKIDITPRERGAGFQFIDKVVGGAVPRNFIPAV
                     GEAAEEAAKKGPFGYPVVDISVTLTDGQFHAVDSSDMAFKTATSMAMKEGLAKADPVV
                     LEPIAKVTISVPNDYTARAQRLLTGRRGQILGFSEKPGWPGWDDVEAMVPEAELHDLI
                     IELRSQTLGLGTYRSSFDHLAEWRGAELKRGAA"
     gene            complement(2716..3222)
                     /locus_tag="VFN42_03495"
     CDS             complement(2716..3222)
                     /locus_tag="VFN42_03495"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HET8995709.1"
                     /translation="MTARPRGVTSRPETSDEFKLEEFLPYRLSVAAHRVSRLFAAQYS
                     EMFDLSIPELRVLATVGRFGAISPSAVGEWAAMDKVKVSRAAGSLVARGLLRQSPHPE
                     DGRGRLLRLTRKGASVQNGVIPLARELEASLSQGLGSTEWRALNRALARLNTHVERIV
                     GRDAEDME"
     gene            3524..>4080
                     /locus_tag="VFN42_03500"
     CDS             3524..>4080
                     /locus_tag="VFN42_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HET8995710.1"
                     /translation="MAGQPSYLSGFGNEFASEAIGGALPVGQNAPQRPPLGLYAEQLS
                     GTAFTVPRREARRTWLYRIRPSARHPAYRRIGNGSLCTPLAEPTPNRLRWDPLPLPQQ
                     PTDFVAGLVTMAANSDTAAATGVSVHLYRANRSMQRVFWNADGELLLVPQQGRLVLTT
                     ELGLLAVAPGEIAVIPRGMRFQVALP"
BASE COUNT          585 a         1464 c         1305 g          726 t
ORIGIN      
        1 tttcgacaac ccggacgacg acctgttcac cgaggaggcg atcctcgcct tggcccgccg
       61 cggcaaaatc ggcgtggtca tcgcggcgat ctgccgtgac ctcggcctgg tgcccagtgt
      121 cgtctccgaa cagcaatgga aggacgtggc cgacctcatc accgacttcg gcggcaattt
      181 cgtcgcactg atcggcgacg cgatgaatcg cgttctcacc tatatccacg cggcgtcgtt
      241 caccaagccg ctgagcggcg cggagatcgg tattctgaac gacgcgatct tcctgcgacc
      301 cgccccggcc tgaccccgag gtggcgtgat gcggtatgca cgggaccctc gtctgggtgg
      361 ccccgcgctt ggcgattcct gttcgatcgg cgacgtgcga tcggtccggc tgcgggtctg
      421 cccgcgccgg ccggtcagca ggcgttgcgc ccgcgcggtg tcgtcgttcg gcgacgctga
      481 ccgtgaccct ggaaatcggt tccagcacca ccggatcagc gttctggttc aggcgacccg
      541 atcgtcgtcg ggtgggtggc cgggtccccc ccggccacga cacacgggct taggcggcgc
      601 cgcgcttcag ctccgcaccg cgccactcgg ccaggtggtc gaacgaactg cgataggtgc
      661 ccaggcccag cgtctgcgag cgcagctcga tgatcaggtc gtgcagctcc gcttccggca
      721 ccatcgcttc cacatcgtcc caccccggcc agccgggctt ctcggaaaag cccaggatct
      781 gcccgcgccg cccggtcaac aggcgctgcg cccgcgcggt gtagtcgttc ggcacgctga
      841 tggtgacctt ggcgatcggt tccagcacca ccggatcggc cttcgccaga ccttccttca
      901 tcgccatgct ggtcgcggtc ttgaacgcca tgtcggagct gtccaccgcg tggaactgcc
      961 cgtcggtcag cgtcacggag atatccacca ccggatagcc gaacgggccc ttcttcgccg
     1021 cttcctccgc ggcctcaccc accgcgggga tgaagttgcg cggcactgcg ccgcccacca
     1081 ccttgtcgat gaactggaac ccggcaccgc gttcgcgcgg cgtgatgtcg atcttcacgt
     1141 cggcgaattg cccgtggccg ccggtctgcc gcttcaggcg cccgtgctcc tgcaccgcct
     1201 ggcggatagt ttccttgtac gccacttgcg gtttgtgcgt gctgaccttc aggttgtagt
     1261 tgctcgccag ccgctgcacc gtgttgttca ggtgcatttc gccctggccg tgcaacacgg
     1321 tttccgcggt gtcctggttc tgctccatcg tcagtgccgg atcttcctcc agcagcttct
     1381 gcagcgcacc ggacagcttc acgtcgtcct tgtgatccgt ggtggcgatg gccagcgagt
     1441 acaccggcgg cggcgggtcc gggaacggca gctgttccgg cgtgccggcc ggcgacacgg
     1501 tcgcgccggt gggcacgccg tcgagccggc ccagcgccgc cacatcgccg gcctgggttt
     1561 ccggcacctt gctcaactcg ccgccggaca ggtgataaat gccgcccagc cgcgtgccgc
     1621 cgagcgtggc accgtccttg atctcgccgc gccagatgcg agcataggac agcttgccgg
     1681 tgtggccggc atgcaccgtc ttgaacacct gcgccagcgc cggcccctcc gcgtcgatgc
     1741 ccttgcgttc ggcggtctcc cgcacgtccg gcacgtcgtg ccgcagcgcc ttccacagcc
     1801 ggcgcacgcc gttggcattc tcggcggagc ccagcagcac ttcgatcacg ctgccggcca
     1861 gcaggtcctt gtgcaggtcg cggtagatct cctgcggcgt cggcttcaca tcttccagca
     1921 ccttctccag cagcacgtcg tcgtggtcgg ccagcacttc gaccagcttg cccagggctt
     1981 cctgttcctc ggcctgcatc tcggtgggga cctgcatcag ctcggacggc tggcccttgc
     2041 ggtagcggta cgcccgctcg ctgatcagat cgacatagcc gttgacgctc tggccctcca
     2101 cgatcggcac ctggcgcagc accagcgggc atttcaccag cccctgcagc gcggccaggg
     2161 tgtcgcgcac gctgccgtcc agcgtgtcga tcttgttgat gaagatcatg tgcggaatgg
     2221 cctcgtccgc caacagtttc agcagcggcg ccagggtcag cgcacgcgac ggcaccggtt
     2281 cgcagaccac cacggcgaaa tccaccatgg cgagcgcggc gcaggcttcg tgcgcgaatt
     2341 cgatggaacc ggggcagtcc agcagcgccc acgggtcatt caggtaagtg cagtggccga
     2401 cacggacctc ggtggtcatc gggcggttgc gcaggtcggc gggccgcttc acaggggcgc
     2461 cggccgcgtc catcaatgcc tcgaacagcg tcgatttccc tgcactgtac gggcccacca
     2521 gagccaccga gcgaggactt ctgccgttgc tggttcctga cacgccgcac ctccttgttg
     2581 gttgttcaag ggtgccggcc tggctttggt cctgttgatg gcaagccaca cggcgccctc
     2641 aaccggcaat cctagcgaag tttttgtgac aggcgagtcc cgccgtgcgt tccctttccg
     2701 ccccggcggc gcgcgctact ccatgtcctc cgcgtcgcgc ccgacaatcc tctccacatg
     2761 cgtgttcagc cgcgccaggg cgcggttgag cgcccgccac tcggtgctgc cgagcccctg
     2821 cgacaggctg gcttccagct cgcgcgccag cgggatgacc ccgttctgca ccgaggcgcc
     2881 tttgcgcgtc aggcgcaaca gccggccacg cccatcctcc ggatgaggcg actgccgcag
     2941 caggccgcgc gcgaccagcg aacccgcggc tcggctgacc ttcaccttgt ccatcgccgc
     3001 ccattcgccc accgcgctgg gcgagatggc gccgaaacgt ccgaccgtgg ccagcacgcg
     3061 caactccgga atgctcaggt cgaacatttc cgagtactgc gcggcgaaca gccgggacac
     3121 gcgatgggcg gccacggaaa gccggtaggg caggaactcc tctagcttga actcgtccga
     3181 tgtctcgggg cgtgaagtta ctccccgggg tcttgccgtc atgctcaatt atcctccagc
     3241 acagtcccag tgtatcgcgg atgttgccgg cagggaacgg gcgacaccgc cgcggcaacg
     3301 cccggaccgg tcccgtccgc ggcgtttatg cagcacggct tcgggtgcac tgcggaacag
     3361 ctggcgcacg actgcggtga atcacgtctc attgaagggt cggcaatgat tccggctcgt
     3421 ttctggaccg ccagaacatt ccggaccgcc tggcggttgc ccggctttgc gatcacgcgc
     3481 cggatcgtca tgctgcgcgc cggcgacagg caggaggacg cgaatggccg gccaacccag
     3541 ctacctctcc ggcttcggca atgagtttgc cagcgaggcg atcggcggtg cgctgccggt
     3601 gggccagaat gccccgcaac gcccgccgct cgggctgtac gcggaacagc tgtccggcac
     3661 cgccttcacc gtgccgcggc gggaggcgcg ccgcacctgg ctctatcgca tccgtccctc
     3721 cgcccggcat cccgcctatc gccgcattgg caacgggagc ctgtgcacgc ccctggcgga
     3781 gccgacgccg aaccggctgc gctgggaccc cttgccgctg ccgcagcagc ccaccgactt
     3841 cgtcgccggc ctggtcacca tggcggccaa ttcggatacg gcagccgcta ccggcgtgtc
     3901 ggtgcatctc tatcgtgcca accgctcgat gcagcgggtg ttctggaacg ccgacggcga
     3961 gctgctgctt gtgccgcaac agggccgcct ggtgttgacc accgaactgg ggctgctggc
     4021 ggtggcgccg ggagaaatcg cggtgatccc gcgcggcatg cggttccagg tggcgctgcc
//
LOCUS       DASSZH010000163         2962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1333363, whole genome shotgun sequence.
ACCESSION   DASSZH010000163 DASSZH010000000
VERSION     DASSZH010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333363"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..1249
                     /locus_tag="VFN42_03505"
     CDS             80..1249
                     /locus_tag="VFN42_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HET8995711.1"
                     /translation="MSNRLPSLDFDLGETADMLRAQVETFAADEIAPRAAAIDRDNDF
                     PADLWTKMGGLGLLGVTVEEEYGGAAMGYLEHVVAMEEISRASASVGLSYGAHSNLCV
                     NQIRRNGNEAQKRRYLPKLISGEHVGALAMSEPGAGSDVVGMRTRAEKRGDRFVLNGS
                     KMWITNGPNADVLVVYAKTDPDAGPRGITAFLIEKGFPGFSTAQKLDKLGMRGSNTCE
                     LVFQDCEVPEENVLSSVGRGVNVLMSGLDYERAVLAAGPIGIMQACMDVVVPYVHERR
                     QFVQPIGEFQLMQGKLADMYTTMNASRAYVYAVAKACDRGQTTRKDAAGAILYAAEKA
                     TWMALEAIQCLGGNGYINDYPTGRLLRDAKLYEIGAGTSEIRRMLIGRELFAETA"
     gene            complement(2550..2813)
                     /locus_tag="VFN42_03510"
     CDS             complement(2550..2813)
                     /locus_tag="VFN42_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET8995712.1"
                     /translation="MGSTLDAFLSAEDIREAAKAEALTRVVAWQLAQEMERQGISKAA
                     LAERMHTSRAQVDRILKAKGNITIETLQRAAALVGRELRLELV"
     gene            complement(2833..>2962)
                     /locus_tag="VFN42_03515"
     CDS             complement(2833..>2962)
                     /locus_tag="VFN42_03515"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET8995713.1"
                     /translation="RPRTVLHEADRIGVLNGFIKKTQKTPADDLDLARRRMKEMQT"
BASE COUNT          463 a          944 c         1080 g          475 t
ORIGIN      
        1 gttcgtgcgc agcggctgac ttgctttccg cgccccccgt cctaaaccct ggcctcccgc
       61 cccggcagga gaggccgcca tgagcaaccg gttgccgtcg ctcgacttcg acctcggcga
      121 gaccgccgac atgctgcgcg cgcaggtgga aaccttcgcc gccgacgaga tcgccccgcg
      181 cgccgccgcc atcgaccgcg acaacgattt ccccgccgat ctctggacca aaatgggcgg
      241 gctcggcctg ctcggggtca ccgtggagga ggaatacggc ggcgccgcca tgggctacct
      301 ggaacatgtc gtcgccatgg aagagatcag ccgagcctcg gcgtcggtgg ggctgtcgta
      361 cggcgcgcat tccaacctgt gcgtcaacca gatccgccgc aacggcaacg aggcgcagaa
      421 gcggcgttac ctgccgaagc tgatctcggg cgagcatgtc ggcgcgctgg cgatgagcga
      481 gccgggcgcc ggatcggacg tggtggggat gcgcacacgg gcggagaagc gcggcgaccg
      541 tttcgtgctg aacgggtcga agatgtggat caccaacggg ccgaacgccg acgtgctggt
      601 ggtgtatgcc aagaccgacc cggatgccgg cccgcgcggc atcaccgcct tcctgatcga
      661 gaagggattc cccgggttct ccaccgcgca gaagctggac aagctgggca tgcgcggatc
      721 gaacacctgc gaactggtgt tccaggactg cgaggtgccg gaggagaacg tgctgagcag
      781 cgtcggccgc ggcgtgaacg tgctgatgag cgggctggac tacgaacgcg ccgtgctcgc
      841 cgccggaccg atcggcatca tgcaggcctg catggacgtg gtggtgccct acgtgcacga
      901 acgccggcag ttcgtccagc cgatcggcga gttccagctg atgcagggca agctggcgga
      961 catgtacacc acgatgaacg cctcccgcgc ctatgtgtat gcggtggcga aggcctgcga
     1021 tcgcgggcag accacgcgca aggatgcggc gggggcaatc ctgtatgcgg cggagaaggc
     1081 cacctggatg gcgctggagg cgatccagtg cctcggcggc aacggctaca tcaacgacta
     1141 cccgacggga cggctgctgc gcgacgccaa gctgtatgag atcggcgcgg ggaccagcga
     1201 gatccggcgg atgctgatcg ggcgggagct gtttgccgag acggcgtagc ggccggttct
     1261 gacgcccgcc gcgatgccgg tggggcaagg cacgaatcgg cagggcggga ccgccatgcg
     1321 gtggccccgt gcctgcctca ccagcctggc gcgcccggtc tggcgcgtcg ccttgccggg
     1381 cggtccggcg cacgcatcat acatgcgccg ccatgctggg agcagcgctg gctgccggtg
     1441 acaaacgccg cgtggttgca ggctgcgcgg atagcgcagc acgtcgcggc aaggctggtg
     1501 gcgcgctcgg cgggctggga ggcacgcgac cggcagatcc ggtttcgccg aggcggcgtt
     1561 ttccggggcg gcgttcgcgg cggcggctcc tgcctgggcg gcatttgtct gctccgtctg
     1621 cccggccgct gcttccggca gcggcccggg gccggaaccg ccaggcggcg gggccagacc
     1681 gccccacagg acggagaaca ccgccttggc atcgctcagt tccgactcag gcggcacgtt
     1741 ctcggcgatg tgccgggcga gcacgcccac cagcagatac gcgaggtcgg gggcgagttt
     1801 gtcgatgggc gtgtttagga tgtcgcaggc ctgctgggtc agccatggat gcgtctgcgg
     1861 agtgaaaggc gcgtcgcggt cagcggccgg cgagcggcgg cgggaccggt cgttccgacg
     1921 cgggcggaga ccgaaccagc gagcgagcca gcctgggcgg ggtgttgcag cggcatcggg
     1981 ctggcgcggg aggcgctgcc gcctggatgg ggtttttgtc ttcggacgat ccgttgtgct
     2041 ggggcgggga gtggaggccg gcgcggcggg acggcggaag cgggcgatgg cgccgcggag
     2101 cgcgccgccg agccgctgga agaagcgcgc accaccccct tgggggtttt gggtagaaag
     2161 gccagcaggg agaggtgcgg tttgcatgcg ccgtcttgta catggtttgt tcctttatgt
     2221 caagcccctt tgggaagatt ttctacgagg ctgggtgggc atgccgccgg ccttcgccgg
     2281 gaagggtgtg catcggcacc gagctttagc cgacatcggc acccaaaact gaccctggca
     2341 accaccgaga acgcctatcc gaatcaactg catgaactca cgcggggtgg gtcgcttttg
     2401 ggcgccgatg ggggggcccg gcgttacgcc ggttcacact ccggcgaaca cagcgggctt
     2461 ggcggccaat ggacaagcgc aagtttaccg ggaagccgtg gatcgccgcc cctggccggc
     2521 aatgacgtgg tggcgcccac ccacgccgct cagaccagct ccagccgcaa ttcgcgcccg
     2581 acgagcgcgg cggcgcgctg gagggtctcg atcgtgatat tgcccttggc cttgaggatg
     2641 cgatcgacct gcgcgcggct cgtgtgcatg cgttccgcca gcgcggcctt gctgatcccc
     2701 tgccgctcca tctcctgtgc aagttgccag gcgaccacgc gcgtcaaggc ctcggctttc
     2761 gcggcttcgc ggatatcttc cgcgctcagg aacgcatcga gcgtcgagcc caatgcgggt
     2821 tcgtcttcct ggtcatgtct gcatctcctt catgcgccgg cgcgcgagat cgagatcgtc
     2881 tgccggcgtc ttctgcgtct tcttgatgaa accatttagc acgccgatcc gatctgcctc
     2941 gtgaagaaca gtacgcggac gg
//
LOCUS       DASSZH010000164         4834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_634600, whole genome shotgun sequence.
ACCESSION   DASSZH010000164 DASSZH010000000
VERSION     DASSZH010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634600"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..970)
                     /locus_tag="VFN42_03520"
     CDS             complement(53..970)
                     /locus_tag="VFN42_03520"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET8995714.1"
                     /translation="MRIAIIGQQDFGKATLEAFLNRGDEVVAVFCAPEKGRPDPLRVA
                     AEQRGVPLFQFARLSDPDAQEAMRAAKADVGVMAYVTQFAPQDFVSIPKFGTIQFHPS
                     LLPLHRGASSMSWSIILGRKETGFSIFRPTDGLDEGPVILMRSVPIEADDTLGSLYFG
                     KIFPMGVSALVEVAELVVAGKAPALAQYEPNAGYEGIIRDAETQINWANHVDITYNLI
                     RGVNPAPGAWTTQDGRKLFLFDAAKRIARTFSEVKGKKLGEVVAQTEKGLLIHGQGGF
                     IEVHRLRWHDGKKIPAQEAGIAVGTVLGA"
     gene            complement(1053..1166)
                     /locus_tag="VFN42_03525"
     CDS             complement(1053..1166)
                     /locus_tag="VFN42_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HET8995715.1"
                     /translation="MLIGGVLLLLAACGKKGPPVPPGPPDKVTWPRSYPTH"
     gene            complement(1199..2632)
                     /gene="argH"
                     /locus_tag="VFN42_03530"
     CDS             complement(1199..2632)
                     /gene="argH"
                     /locus_tag="VFN42_03530"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003628278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET8995716.1"
                     /translation="MDDTPHLPNTPANQQWGGRFAAGPSAIMQEINASIGFDQKLWRQ
                     DIRGSLAHAAMLAKVGVISGEDERAIHAGLEAIAVEIEAGRFPFDAALEDIHMNIEAR
                     LTDRIGDAGRRLHTARSRNDQVATDFRLWVRDAIDGLSAQIADVMLALAERAAAHAAD
                     PMPGFTHLQTAQPVTFGHHLLAYVEMLARDRGRLADCRGRLNECPLGSAALAGTSFPI
                     DRHMTAQALGFDRPTANSLDAVSDRDFALEFLAAAAISAMHLSRFAEEIIIWCSAPFR
                     FIRLSDAFTTGSSIMPQKRNPDAAELVRAKTGRVTGSLIALLTVMKGLPLAYAKDMQE
                     DKQPVFEAAEAWALSLAAVAGMARDMTPDTGRMRALAGSGFSTATDLADWLVRTLKLP
                     FRNAHHVTGRLVALAEARGVDLADLPLADMQQVEQSITPDVFSVLSVDASVASRRSVG
                     GTAPENVAREAARWLAALQAPQQAGAR"
     gene            2588..3205
                     /locus_tag="VFN42_03535"
     CDS             2588..3205
                     /locus_tag="VFN42_03535"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET8995717.1"
                     /translation="MLVGRRIGKVGRIVHWKESGMLITRRRMLATAAAGGTLAAGLTP
                     RKLRAAQLNSVSSALKLIVPAMAPPAITFLDAAGKPHHLSEFLGHGMLINLWATWCPP
                     CVEEMPSLAVMAKALAPNNMAVLPLSADNGGAKAVEAFFARRKITGLPVLLDPDGAAA
                     RAFNVPGLPTSIVIGKKGLQLARAVGAVNWAAPEAASIVQRLAEG"
     gene            complement(3228..4094)
                     /locus_tag="VFN42_03540"
     CDS             complement(3228..4094)
                     /locus_tag="VFN42_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HET8995718.1"
                     /translation="MPIDSIVLTRRSALAIAGAACLARPGLAAPTWTRGAYEAAMQAS
                     GRPVSLTDAQFDAIQKRKPAAMRRIADYLQRHLGSADPAVMAAFEAVPREYFHYLYQA
                     HRATPGDAYETDPKPWAIGYGSALSDYLGQAYMTQVCQPKPGQTTLEIGTGSGFQSAL
                     LSRIVARAYSIEIIEPLGKAVGRIFAPIGYDNVHTKVGDGYYGWPEVEGGFDVIIVTC
                     AATYAPPELFKQMKPGGRLVIPIGQPFKRGQVLYVYTKDAEGKIHSHRDMGVFFIPMT
                     GAISRAPRPHGT"
     gene            complement(4122..>4834)
                     /locus_tag="VFN42_03545"
     CDS             complement(4122..>4834)
                     /locus_tag="VFN42_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995719.1"
                     /translation="ALFAVLVLLVPAMARRRIRIARSGLLRPVLYFPALGLGFLFIEI
                     ALIEKASLWLNDRTSGFALVLTGMLVFSGIGSMAADRLAGRARMSLTVAGGLVLVWCA
                     AVAAGLPPAILATLDWPWAARVALLTLVLAPASLAMGLPFPLGLSAIGEGAELAWAWG
                     LNGAFSVLATPLAHLVAREWGFHTVILAAALLYLLAVASFPAAPAAVLRSAHSRTESP
                     AQQSRIGLEGSVVHRESV"
BASE COUNT          789 a         1627 c         1661 g          757 t
ORIGIN      
        1 agacggcgta gcggccgcac atcggtcagg ctttcggctg cggctgatgg cgtcaggcgc
       61 cgaggacggt gcccaccgcg atccccgctt cctgggccgg gatctttttg ccgtcgtgcc
      121 agcgcaggcg gtgcacctcg atgaagccgc cctggccgtg gatcagcagc cccttctcgg
      181 tctgtgccac cacttcgccc agcttctttc ccttcacttc ggaaaaggtg cgggcgatgc
      241 gcttggcggc atcgaacagg aacagcttgc ggccgtcctg cgtggtccag gcaccgggcg
      301 cggggttcac accgcggatc aggttgtagg tgatatcgac gtggttggcc caattgatct
      361 gggtttccgc gtcacggatg atcccttcat agccggcatt cggctcatat tgcgcgagcg
      421 cgggcgcctt gccggcgacc accagctcgg cgacctccac cagcgcggac acgcccatcg
      481 ggaagatctt gccgaaatac agcgagccca gcgtgtcgtc ggcttcgatc gggaccgagc
      541 gcatcaggat caccggtccc tcgtccagcc cgtcggtggg acggaagatg ctgaatccgg
      601 tctccttgcg gccgaggatg atcgaccagc tcatcgacga cgcgccgcga tgcaatggca
      661 acagcgaagg atggaactgg atggtgccga acttcgggat gctgacgaag tcctgcggag
      721 caaattgcgt gacataggcc atcacgccga catcggcttt ggcggcacgc atcgcttcct
      781 gcgcatcggg gtcgctcagc ctggcgaact ggaacaacgg cacgccccgc tgctctgcgg
      841 cgacgcgcaa cgggtcagga cggcccttct ccggcgcgca gaacaccgcg accacctcgt
      901 cgccgcgatt gaggaatgcc tccagcgtgg ccttgccgaa atcctgctgg ccgatgatgg
      961 cgatgcgcat gcgttgcctc ccgcttctgt gcaggaagtt tcggctgcag tgcgtgggat
     1021 gtcgaggggc gggcggccga gtccggtccg gttcagtgcg tcgggtagga gcggggccag
     1081 gtcaccttgt ccggcggtcc cggtggcacc ggcggaccct tcttgccgca ggccgccagc
     1141 agcaacagta cgccgccgat cagcagcgtc cgcgcgaccg tgcgggtcca gcggcggctc
     1201 accgcgcgcc cgcctgctgc ggggcctgca atgccgccaa ccagcgtgcc gcctcgcgcg
     1261 ccacgttctc cggagccgta ccgccaacgc tgcgccgtga tgccacggag gcgtccacac
     1321 tcagcacgga aaacacatcc ggcgtgatgc tctgctccac ctgctgcatg tccgccagcg
     1381 gcaggtccgc caggtcgacg ccgcgcgcct cggccagcgc caccaggcgc ccggtgacgt
     1441 ggtgcgcgtt gcggaacggc agtttcagcg tgcgcaccag ccagtccgcc aggtcggtgg
     1501 cggtggaaaa tcccgacccg gccagcgcgc gcatgcggcc ggtgtccggc gtcatgtcgc
     1561 gcgccatgcc ggcgacggcg gcgagcgaca atgcccaggc ctcagccgct tcgaacaccg
     1621 gctgcttgtc ctcctgcatg tccttggcat aggccagcgg caggcccttc attacggtca
     1681 gcagcgcgat caaggatccg gtcacccggc cggtcttcgc ccgcaccagc tccgccgcat
     1741 cgggattgcg cttctgcggc atgatggagc tgccggtggt gaaggcgtcg ctcaaccgga
     1801 tgaagcggaa gggcgcgctg caccagatga tgatctcctc ggcgaagcgc gacaggtgca
     1861 tggcggaaat cgcagccgcc gccaggaact ccagcgcgaa gtcgcggtca gacaccgcgt
     1921 cgagcgagtt ggcggtgggc cggtcgaagc cgagcgcctg cgcggtcatg tgccggtcga
     1981 tcgggaagga ggtgccggcc agcgccgccg atcccagcgg acattcgttc agccgccctc
     2041 ggcaatcggc cagccggccg cggtcgcggg ccagcatctc gacataggcc agcagatgat
     2101 ggccgaaagt gaccggctgt gccgtctgca gatgggtgaa gcccggcatc gggtcggcgg
     2161 cgtgcgcggc ggcccgttcc gccagcgcca gcatcacgtc ggcgatctgc gccgacagcc
     2221 cgtcgatcgc gtcgcgcacc cacaggcgga aatcggtcgc cacctggtcg ttgcggctgc
     2281 gcgcggtgtg caggcggcgg ccggcatcgc cgatccgatc ggtgaggcgc gcctcgatgt
     2341 tcatgtggat gtcttccagc gcggcgtcga acgggaagcg gccggcctcg atctccacgg
     2401 caatggcctc caggccggcg tggatcgccc gttcgtcttc gccgctgatc acaccgacct
     2461 tggccagcat ggccgcatgc gccagggatc cgcggatgtc ctgccgccag agcttctggt
     2521 cgaagccgat cgaggcgttg atctcctgca tgatcgccga ggggccggcg gcgaagcgcc
     2581 cgccccattg ctggttggcc ggcgtattgg gaaggtgggg cgtatcgtcc actggaagga
     2641 atccggaatg ttgataacgc gccgcagaat gctggcaaca gcggcagcgg gcgggaccct
     2701 agccgccggc ctgacgccgc gcaaactgcg cgccgcacaa ctgaactcgg tgtcctccgc
     2761 cctcaaactg atcgtgccgg ccatggcgcc gcccgccatc acctttctcg atgccgcggg
     2821 caagccgcat cacctgtcgg agttccttgg gcacggcatg ctgatcaacc tttgggccac
     2881 ctggtgcccg ccctgcgtgg aggaaatgcc gtcgctcgcg gtaatggcca aggcgctggc
     2941 gccgaacaac atggccgtat tgccgctgtc cgccgacaac ggcggcgcaa aggcggtgga
     3001 agcgttcttt gcgcgccgga agatcaccgg attgcccgtc ctgttggatc cggatggcgc
     3061 cgcggcccgg gccttcaacg tgccgggcct gcccaccagc attgtgatcg gcaagaaggg
     3121 cctacagctt gcccgtgcgg tgggcgcggt gaactgggcg gcgccggagg ccgcgagcat
     3181 cgtgcagcgc ctggcagaag gctgaacccg gacgcgtccc gcccagtcta cgtgccgtgc
     3241 ggccgggggg cacggctgat cgcgccggtc atgggaatga agaagacgcc catgtcgcgg
     3301 tgggagtgga tcttgccctc cgcgtccttg gtgtagacgt acagcacctg tccccgtttg
     3361 aacggctgcc cgatcgggat caccagccgg ccgcccggct tcatctgctt gaacaactcc
     3421 ggcggggcat aggtcgcggc acaggtgacg atgatcacgt caaagccgcc ttccacctcc
     3481 ggccagccgt aatatccgtc gccgaccttg gtatgcacgt tgtcgtagcc gatcggcgcg
     3541 aagatgcggc cgaccgcttt gccgagcggc tcgatgatct cgatcgaata ggcccgggcg
     3601 acgatccgcg acagcagagc gctctgaaaa ccgctgccgg tgccgatctc cagtgtggtc
     3661 tggccgggtt tcggttggca gacctgagtc atataggcct gccccagata gtccgacagg
     3721 gcggagccgt agccgatcgc ccagggcttc ggatcggtct cgtatgcgtc gcccggcgtg
     3781 gcccggtgcg cctggtaaag gtagtggaag tactcgcgcg gcaccgcttc gaacgccgcc
     3841 atcaccgccg gatcggccga gccgagatgg cgttgcaggt aatccgcgat gcgccgcatc
     3901 gccgcgggct tgcgcttctg gatggcgtcg aactgagcgt cggtcaggct gaccgggcgg
     3961 ccggatgcct gcatggccgc ttcataagcg ccgcgggtcc aggtgggagc ggccaggccg
     4021 gggcgggcca gacaggcggc gccggcgatg gcaagcgcgc tgcgacgggt caggacgatg
     4081 gaatcgattg gcacgcccga caactcctct gcggcgcccc ttcatacgct ttcgcggtgc
     4141 acaacagaac cttccaggcc gattctggac tgttgcgctg gggattcagt acggctgtgg
     4201 gcgcttcgca acacagcggc cggtgccgcc ggaaagcttg caacggcgag cagatagagc
     4261 aaggcggcgg ccaggataac ggtgtggaag ccccattcgc gcgcgaccag gtgtgcgagc
     4321 ggcgtcgcga ggacggagaa ggcgccgttg agcccccagg cccaggccaa ttcggcgcct
     4381 tcgccaatgg cggacaggcc cagcgggaac ggtagcccca ttgccagcga cgccggcgcg
     4441 agcaccaggg tcagcaaagc gacgcgtgct gcccagggcc agtccagcgt cgccaggatc
     4501 gccggcggca gtcccgcggc gactgccgca caccacacca gcaccaggcc gcccgcgact
     4561 gtcaggctca tgcgggctcg gccggccagc cggtccgcgg ccatgctgcc gatgccggaa
     4621 aagaccagca ttccggtcag caccagggcg aagccgctgg tgcggtcgtt cagccacagg
     4681 ctcgccttct cgatcagagc tatttcgatg aacaggaacc cgagtcccag cgcggggaaa
     4741 tacaacacgg gccgcagcaa gccggagcga gcgatccgga tccgccggcg tgccattgca
     4801 ggcaccagga gcaccagcac agcgaacagc gcgg
//
LOCUS       DASSZH010000165        11352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1486785, whole genome shotgun sequence.
ACCESSION   DASSZH010000165 DASSZH010000000
VERSION     DASSZH010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 11352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11352
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486785"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VFN42_03550"
     CDS             <1..217
                     /locus_tag="VFN42_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010668029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8995720.1"
                     /translation="DTPLLQRSLHIPGFAEATNAEIPMGRLGQPEEMANVVLFLASDL
                     ASYVTGAAFVADGGLTAKTGVPTRFRT"
     gene            314..1138
                     /locus_tag="VFN42_03555"
     CDS             314..1138
                     /locus_tag="VFN42_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995721.1"
                     /translation="MTNAPALSEPFSKPRQGIGARLARGFGGVVRKAFAVLSHPRCRQ
                     PRAAAPAGGNAPASRPGQSSLSRKRQPRPAPAASVAPPARRGWLARCFGLRRGPRPAS
                     RPCRSPDAPFTPETCPGLTAEDCAFLNTPVEDCDPEVLRQLLGALAQRIAAILPPDSG
                     IADAQAMFAMLWTRLGAPAAQPDATAAPQQQPAGQALSRRPSRHIGSRRRRPAAATGD
                     AGRAALALPGHRRRARHRVKLWDFPAGNHAPRRTAPGTGQRLGIRRLCYAARASPP"
     gene            complement(1277..1687)
                     /locus_tag="VFN42_03560"
     CDS             complement(1277..1687)
                     /locus_tag="VFN42_03560"
                     /inference="COORDINATES: protein motif:HMM:NF040444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HET8995722.1"
                     /translation="MAQQSTEQKKTVSRVMHEYKHGELERGRGGKVKSRRQAVAIALS
                     EAGASNQESPRENRRHYAHTKRKERRGETAQQEKEGRRAMDRSQSRRRRSASAARRTG
                     GGRTRAQLYQEAQRRKIPGRSKMNKAQLERALRG"
     gene            complement(1746..2249)
                     /locus_tag="VFN42_03565"
     CDS             complement(1746..2249)
                     /locus_tag="VFN42_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET8995723.1"
                     /translation="MANHKGFNELMLSFMQDIYYAERQILKALPKMAKAAQNEKLKQA
                     LLHHREETQGQVERLQKAFEALGKRARAQTCEAINGIIEEGEETVEEFEQGDVRDAGI
                     IANAQAVEHYEMARYGTIVAWAKACGQKEVAQLMQETLEEEKKADTLLNDLANKQVNQ
                     SATRQAA"
     gene            complement(2454..3053)
                     /locus_tag="VFN42_03570"
     CDS             complement(2454..3053)
                     /locus_tag="VFN42_03570"
                     /inference="COORDINATES: protein motif:HMM:NF015642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HET8995724.1"
                     /translation="MSQSPPNPPVDLDTLDAFLDSDRAPADCMDVSEIDGFLAALVVG
                     PEPIGWDEALPVIWGGETPDFTDADESAVVTGTIVARHGEIATLLDTDPEDYAPVFLE
                     DYAGTTITEDWALGFMQAVSLRADAWKPLLRDDESAMLLIPIGIMAGMADPEIRLDTG
                     LPDGLMDELVANAESVLPACVRGLHAYWRQRRMNGVTRN"
     gene            complement(3153..3863)
                     /gene="ccmI"
                     /locus_tag="VFN42_03575"
     CDS             complement(3153..3863)
                     /gene="ccmI"
                     /locus_tag="VFN42_03575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HET8995725.1"
                     /translation="MIWLAAAILAGVALMPLLWSLRRSITLRGRREAALAFHRAQLAE
                     LERDLAEGRIAAEDHAGAVLEVQRRVLAASTDQEPLPPGSPRSPLLITLILVPLAAFA
                     LYAIGGSPDLPSEPLAQRIAAAQVREQRENALIALLRHKLSTLDPHTVLARQGYVLLG
                     NAEARMGRMQQAADAWQTALADRFDPTLAAETGEAMTEAAGHVTRQAAALFRHALKDA
                     PADAPWRPMAVKRLGGAN"
     gene            complement(3874..4353)
                     /locus_tag="VFN42_03580"
     CDS             complement(3874..4353)
                     /locus_tag="VFN42_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HET8995726.1"
                     /translation="MRLRRAQAAPILVALLAMLCLLPPPAAHALSDPSEMLANPAQEK
                     RAEQIGDQLRCLVCQNESIEQSGAGLARDLRHIIRQQVAAGKTNKQIIAWMVARYGDF
                     VLLKPPFAPATLLLWGAPVLALCAGTGAILLARRRPAATPAPLTDEERQRATELLGQ"
     gene            complement(4350..4919)
                     /locus_tag="VFN42_03585"
     CDS             complement(4350..4919)
                     /locus_tag="VFN42_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HET8995727.1"
                     /translation="MTPLQRRALLLAPLGLAAIGGSAMWVLLARLNDGGYDPHAVPDP
                     MVGKPVPRFSLPGLGAGPGFTSAELVKHAPLLLNFFASWCLPCAQEATELMKLKQRGL
                     PIWGVVYKDKPEAAAGFLKQNGNPYRRVAVDQPGLTAIDFGLYGVPETYLIDKHGILR
                     WRWAGGLSSTVVRDWLDPRLKTYARELLS"
     gene            complement(4916..6886)
                     /locus_tag="VFN42_03590"
     CDS             complement(4916..6886)
                     /locus_tag="VFN42_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019090443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HET8995728.1"
                     /translation="MIPELGHFALALALAVAAAQAVLPLWGAHDRDSRLMASAPALAL
                     GQMIALLTSYACLVWSAVVDDFSVLNVAENSNATKPLLYKITGTWGDHEGSILLWCLI
                     LSLCGGAVALFGRRLPSSLRARVIGVLGCVSAGFLLFTLTASNPFQRLWPPPINGSGM
                     NPLLQDPGLAFHPPVLYAGYIGFSIPFAFAIAALLEGRVDAAWGRWVRPWTLGAWSLL
                     TCGIALGSWWAYYELGWGGYWFWDPVENASLLPWLAGTALLHSAIVVEKRESLKIWTI
                     LLAIGTFSLSLCGTFLVRSGILNSVHAFAVSPTRGVFILGLLAIYIGASLLLFAFRAP
                     SLAPTGIFAPISREGALVLNNVLLCSICAVVLTGTTYPLFSALFGDKLSVGAPYFEMT
                     VFPLCIPLFAAMSVGPVMPWKRAALWPALRRLWWAALAAAAVAVVVAFGVHRVGTALG
                     FAAASWLILGALAEPVQRVRLFRVKPSHSWARATAQPLAVWGSALAHAGLGVTVAGIA
                     GMTLAMHLIVAVAPGQTAHLDGYDFTLLDLHNATGPNYTARVADLNITRDGHEVALLH
                     PMRRHFTVQKETTADTAIETNGLRDLYTVLGGERDGKALLRLNVNPFGPWIWFGALVM
                     AAGGALSLADRRLRIGAPARRRRDAHAYGPAE"
     gene            complement(7315..7473)
                     /locus_tag="VFN42_03595"
     CDS             complement(7315..7473)
                     /locus_tag="VFN42_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995729.1"
                     /translation="MRSPKRDVNPSNLGFRAFVVPVAWIVALVASYWLIADWQTLPGL
                     VSNAIAAI"
     gene            complement(7706..8662)
                     /locus_tag="VFN42_03600"
     CDS             complement(7706..8662)
                     /locus_tag="VFN42_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010513520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HET8995730.1"
                     /translation="MRNFSDLTEREVLALAIANEEEDGRIYLDIAEGLRQDYPGSANV
                     FTEMAAEEGEHRRRLLELYREKFGEHIPLIRRQDIRGFIHHPPVWQLHPLQLEQVRNL
                     AESMEQETQQFYRRAATRSQDVSIRKLLGDLADAEVEHEHRADALRTENLPADVVEKE
                     NLTAHRSFVLRVIQPGLAGLMDGSVSTLAPVFAAAFASNQSWEAFLVGMAASIGAGIS
                     MGFAEALSDNGSLTGRGAPLMRGLITGLMTTLGGIGHTLPFLIPYFWTATAIAIVVVV
                     IELAVISWVRWKYMDTPPFSAAMQVAFGGALVFGCGILIGNS"
     gene            8911..9939
                     /locus_tag="VFN42_03605"
     CDS             8911..9939
                     /locus_tag="VFN42_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8995731.1"
                     /translation="MPSITRRRFGLQMLAGSAALAGTRGTPAYAADKVHVGILPLTSH
                     APTIIASAKGYFAAQGLEATLVSFEAAQPMAIAIASGDVDFGVTAITGGLISLADKGA
                     VKVIGGALQEYPNVEGQKILASKAAYDKGLTSPAQLKVHSFGITTAGSSFQYMAHKIA
                     QKQGWPDSVIPLRPLQSVPNVVAALKSGQIDAWSIVPNIADALTKGGDVKQIGKIADF
                     IPDYQVTVVFTSTANVTKRPALVKRFLAAYEKGASDYNAALVDRTMGPKETADIVAMI
                     HKYVYASVPLDKAAPRIKHGAMRINPGARMNLASIKDQLDWFKAEHMVPVGVTMEQLV
                     NTSFVKAG"
     gene            10083..10853
                     /locus_tag="VFN42_03610"
     CDS             10083..10853
                     /locus_tag="VFN42_03610"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8995732.1"
                     /translation="MELSIENITHAYDATKVLEDISFTVRPGEIVCIVGPSGCGKSTL
                     LRFVGGLERPDAGRVMLLGDPPAGSLNPLTYIFQDFALLPWRTVEGNVSLVLEDHGMP
                     RAQARAIIDDVLARTNLSDFRKAWPRQLSGGMKQRVAIARALSVRPAVMLMDEPLSAL
                     DSQTRELLMDDLVELWSREGFSACYVTHNLAEAVRLGHKIIVLSRRPGRIREVVTIDM
                     PLSERPERGAELDRLQRQVWQIMREEARQADMELTDAG"
     gene            10843..>11352
                     /locus_tag="VFN42_03615"
     CDS             10843..>11352
                     /locus_tag="VFN42_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8995733.1"
                     /translation="MLDEPVQQRPAAAAGPGEPARPEARAVPFRGGGFRAKRQPLVAW
                     LVFAALLLLWEAGSRTGLISNIVLPAPSEAFAAFMDLVRTGMLWKHLDASLYRLIVGW
                     TAGTALGIVVGLLIGLSSLARAGLLQLVSALFAIPKIALLPLFVVWFGIGEGSKIATI
                     LFGTFFPTVI"
BASE COUNT         1790 a         3948 c         3865 g         1749 t
ORIGIN      
        1 cgacacgccg ctgctgcagc gctcgctgca tattcccggt ttcgccgagg cgaccaacgc
       61 ggagattccg atgggccggc ttggccagcc ggaggagatg gcgaacgtgg tgctgttcct
      121 cgcctctgac ctcgcgtcct acgtcaccgg cgccgctttc gtggcggatg gcgggctgac
      181 cgcaaagacc ggggtgccga cgcggttccg cacctgacgc ggcagccggc acggccggcc
      241 agccacgtct ggagaggaag ctcctaggca tatggtcttg acaacggcgc gggaatgtgc
      301 ataatgtctt gccatgacaa acgcacccgc cctttccgag cctttttcca aaccccgcca
      361 ggggataggt gcgcgcctcg cccggggctt cggcggcgtg gtccgcaagg ctttcgccgt
      421 cctctcgcat ccccgctgca ggcaaccccg tgccgctgcg ccagccggcg gcaatgctcc
      481 ggccagccgg cccggccaat catccctttc gcgcaagcgc cagccccggc cggcgcccgc
      541 tgcatccgtc gcgccgcccg cgcggcgcgg ctggctcgcc cgctgcttcg gcctccgccg
      601 cggcccacgc ccggcctccc gcccttgccg gtcacccgac gcgccgttca cgccggaaac
      661 ctgccccggg ctcaccgccg aagactgcgc gttcctcaac acgccggtcg aggactgtga
      721 tcctgaggtg ctgcgccagt tgctgggcgc tttggcccag cggatcgcgg ccattctgcc
      781 gccggactcc ggcatagccg atgcccaggc gatgttcgcc atgctgtgga cgcgccttgg
      841 tgcccccgcc gcccagccgg acgcaacggc cgccccgcag cagcaacccg ccggccaggc
      901 cctgtcccgc cggccctccc gtcacatcgg aagccggcgg cgccggccgg ccgccgccac
      961 cggcgatgcc ggcagggccg ccctcgccct gccggggcac cgccgccgag cgcggcatcg
     1021 tgtcaaactc tgggattttc ctgcgggaaa tcacgcgccg cgccgcaccg cgcccggcac
     1081 aggccaacgc ctgggcatcc ggcggctctg ctacgccgcc cgcgcgagtc cgccctgact
     1141 ccgtggcgcg ccattccagg cgcgccacac gtcgcgcgaa ctcacgggac caccgcccgg
     1201 cggtcccgcc ataccgattc cgccaatgcc cagcgtcccg aatggccggg acgcagcgcc
     1261 gggcctcgcc cgcggatcag ccgcgcaatg cgcgttccag ttgcgccttg ttcatcttcg
     1321 accggccggg gatcttgcgc cgctgcgctt cctggtagag ctgcgcccgc gtccgcccgc
     1381 cgccggtccg ccgcgcggcc gaagcgctcc gccggcgccg cgactggctg cggtccatcg
     1441 cccggcggcc ttccttctcc tgctgcgcgg tctcgccacg gcgctccttg cgcttggtgt
     1501 gcgcatagtg ccgccggttc tccctgggcg attcctggtt ggacgccccg gcctcgctca
     1561 gcgcaatcgc caccgcctgg cgccggctct tcaccttgcc gccgcgccca cgctccagct
     1621 cgccatgctt gtattcgtgc atgacgcggg agacggtctt cttctgctcg gtgctctgct
     1681 gtgccatctg caacaacctc cgcggaaatg gccgggggcc ggtcgatgcc cccggccggc
     1741 gcgtatcagg cggcctgcct ggtggcgctt tggttcacct gcttgttggc caggtcgttc
     1801 agcaaggtat ccgccttctt ctcttcctcc agcgtctcct gcatgagctg cgcgacttcc
     1861 ttctggccgc aggccttggc ccaggcgacg atcgtgccgt agcgcgccat ctcatagtgt
     1921 tccaccgcct gggcattggc gatgatgccg gcgtcgcgca cgtcgccctg ctcgaactct
     1981 tcgacggtct cctcaccctc ctcgatgatg ccgttgatcg cctcgcaggt ctgggcgcgg
     2041 gcgcgcttgc ccagcgcctc gaacgccttt tgcagacgct ccacctggcc ctgggtctcc
     2101 tcccggtggt gcagcagcgc ttgtttcagc ttctcgttct gcgccgcctt cgccatcttc
     2161 ggcagcgcct tcaggatctg ccgttcggcg taatagatgt cctgcatgaa cgacagcatc
     2221 agttcgttga agcccttgtg gttcgccatg gtttgccctc gtctggaatg cggccgcgcg
     2281 cggcagccgg cctctgctgc ccgttgcgtg cgcaaaccgg ggcaccaccg atccccgcgt
     2341 gttttctggg cggtatccgg ctggcttgcg gcgcaagggg caccagacaa accaacaccc
     2401 aggcgccgcc gttcacacgc ggcggcgcaa aaacccgtgt gatttggact atgtcagttc
     2461 cgggtaacgc cgttcatccg gcgttgccgc caataggcat gcaggccgcg aacgcaggcc
     2521 ggcagcacgc tctcggcatt ggccaccagc tcatccatca gcccgtcggg caggccggtg
     2581 tccaaccgga tctccggatc cgccatcccg gccatgatgc cgatcgggat cagcagcatg
     2641 gcggattcgt catcgcgcag cagcggcttc caggcatccg cacgcaggct cacggcctgc
     2701 atgaagccga gcgcccagtc ctcggtgatg gtggtgccgg cgtagtcctc caggaagacc
     2761 ggcgcgtagt cctccggatc ggtgtccagc agcgtggcga tctcgccatg ccgggcgacg
     2821 atggtgccgg tcacgacggc ggactcgtct gcgtcggtga agtcgggcgt ctcgccgccc
     2881 cagatcaccg gcagcgcctc gtcccagccg atcggctccg gaccgaccac cagcgcggcg
     2941 aggaatccgt cgatctccga cacgtccatg cagtcggccg gtgcccggtc ggagtccagg
     3001 aacgcgtcca gcgtgtcgag atcgaccggg gggttaggcg gggattgaga catgggacac
     3061 tgccttccaa tcagcttatg tagcccggcg gcgcccggtt cccatagggc accgtgcggg
     3121 tgcggctgcc cgcggcggga ttgttccggc gcctagttcg ccccgcccag ccgcttcacg
     3181 gccatcggcc gccagggcgc gtcggccggc gcatccttca gggcatgccg gaacagggcg
     3241 gcggcctgcc gggtgacatg gccggccgcc tcggtcatcg cctcgccggt ttccgcggcc
     3301 agcgtggggt cgaaccggtc ggccaatgcc gtctgccagg catcggccgc ctgctgcatg
     3361 cggcccatgc gggcctcggc attgccgagc agcacgtagc cctggcgggc cagcacggtg
     3421 tgtggatcca gcgtggacag cttgtgccgc agcagcgcga tcagcgcgtt ctcccgctgc
     3481 tcccgcacct gggcggcggc gatgcgctgc gccagcggtt ccgacggcag gtcgggggaa
     3541 ccgccaatgg catacagggc gaaggccgcc agcggcacca ggatcagcgt gatcagcagc
     3601 ggcgagcgcg gactgcccgg cggcagcggt tcctgatcgg tgctggcggc cagcacccgg
     3661 cgctgcactt ccagcacggc gccggcatgg tcttccgcgg cgatgcggcc ttccgccaga
     3721 tcgcgctcca gctcggcgag ttgcgcgcga tggaaggcca gtgcggcctc ccgccggccg
     3781 cgcagcgtga tgctgcggcg gagcgaccat agcagcggca tcagcgccac gccggccagg
     3841 atggcagcgg ccagccatat catgcgggtg gtctcactgg cccagcagct ccgtcgcgcg
     3901 ttgccgttcc tcgtcggtca gcggcgccgg ggtggcggcg ggccggcgcc gcgccagcag
     3961 gatcgcgccg gtgccggcac acagcgccag gacgggtgca ccccacagca gcagggtcgc
     4021 gggggcgaaa ggcggcttca gcaggacgaa atcgccatag cgcgcgacca tccaggcgat
     4081 gatctgcttg ttggttttcc ctgcggccac ctgctggcgg atgatgtggc gcaggtcgcg
     4141 ggccagcccg gcgccggatt gttcgatgct ctcgttctgg cagaccaggc agcgcaactg
     4201 gtcgccgatc tgttcggcac gtttctcctg tgccggattg gccagcatct cgctcggatc
     4261 gctcagcgcg tgggcggccg gcggcggcag caggcagagc atcgcgagca gggcgaccag
     4321 gatcggcgct gcttgggcgc ggcgcagcct catgacagga gctcgcgggc ataggtcttc
     4381 agccgcggat cgagccagtc acgcacgacg gtggaactca ggccgcccgc ccagcgccag
     4441 cgcagaatgc cgtgcttgtc gatcaggtag gtttccggca cgccgtacag gccgaaatcg
     4501 atcgcagtca ggccaggctg gtccaccgcg acacggcggt acggattgcc gttctgcttc
     4561 aggaagccgg cggcggcttc tggcttgtcc ttgtacacca cgccccagat cggcaggccg
     4621 cgctgcttca gcttcatcag ttcggtggct tcctgcgcgc agggcaggca ccaggaggcg
     4681 aagaagttca gcagcagcgg cgcatgcttc accagttcgg cgctggtgaa gcccgggccg
     4741 gcgcccagcc cgggcaggct gaaccgcggc accggcttgc cgaccatcgg atccggcacg
     4801 gcgtgcgggt cgtagccgcc gtcgttcagc cgcgcgagca gcacccacat ggcgctgccg
     4861 ccgatcgccg ccaagccgag tggcgccagc agcaaggctc tgcgctgcag cggagtcatt
     4921 cggcgggccc gtaggcgtgg gcgtcgcgcc ggcgccgcgc cggggcgccg atgcgcagtc
     4981 gccggtcggc caatgacagc gcgccgcccg cggccatcac cagggcgccg aaccagatcc
     5041 acggaccgaa tggattgacg ttcagccgca ggagcgcctt gccgtcgcgc tcgccgccca
     5101 gcaccgtgta caggtcgcgc agcccgttgg tctcgatcgc ggtatcggcg gtggtctcct
     5161 tctgcaccgt gaagtgccgg cgcatgggat gcagcaatgc cacttcatgc ccgtcgcggg
     5221 tgatgttcag gtcggccacc cgggcggtgt agttcggccc cgtcgcgttg tgcaggtcca
     5281 gcagggtgaa atcatagcca tccagatgtg cggtctggcc gggcgcgacc gcgacgatca
     5341 ggtgcatcgc cagcgtcatg ccggcgatgc cggccacggt gacgccaagc ccggcatggg
     5401 cgagcgccga tccccagacg gccagcggct gggcggtggc cctggcccag gaatgcgacg
     5461 gcttcacgcg gaacagccgc acccgctgca ccggctcggc caaggcgccc aggatcagcc
     5521 aggaagcggc ggcgaatccc agggcggtgc ccacccggtg gacgccgaat gccaccacca
     5581 ccgcgaccgc cgccgccgcc agtgcggccc accacagacg ccgcagggcc ggccacagcg
     5641 cggcgcgctt ccacggcatc accgggccaa ccgacatcgc ggcgaacagc gggatgcaca
     5701 gcgggaagac ggtcatctcg aaatacggcg cgcccacgct gagcttgtcg ccgaacagcg
     5761 cggagaacag cggataggtc gtgccggtga gcaccacggc gcagatcgaa cagagcagca
     5821 cgttgttcag caccagcgcg ccttcgcgcg agatcggcgc gaagatgccg gtgggcgcca
     5881 gggacggcgc gcggaacgcg aacagcagca ggctggcgcc gatatagatt gccagcaggc
     5941 ccaggatgaa cacgccgcgg gtcggtgaca cggcgaaggc gtgcaccgaa ttcaggatgc
     6001 cggagcggac caggaaagtg ccgcacagcg acagcgagaa ggtgccgatc gccagcagga
     6061 tggtccagat cttcagcgac tcgcgctttt ccaccacgat ggccgagtgc agcagcgcgg
     6121 tgccggcgag ccagggcagc agcgaggcat tctcgaccgg gtcccagaac cagtagccgc
     6181 cccagccgag ttcgtaatag gcccaccagg aacccagcgc gatgccgcag gtcagcagcg
     6241 accaggcgcc caatgtccag gggcgcaccc agcggcccca ggcggcatcc acccggcctt
     6301 ccagcagcgc ggcgatggcg aaggcgaagg gaatggagaa gccgatatag ccggcgtaga
     6361 gcaccggtgg gtggaaggcg agacccggat cctgcagcag cgggttcatg ccgctgccgt
     6421 tgatcggtgg cggccatagc cgttggaacg ggttggacgc ggtcagcgtg aacagcagga
     6481 agccggcgga gacgcagccc agcaccccga tcacccgggc gcgaagcgag gagggcaggc
     6541 gccgaccgaa cagcgccacc gcgccgccgc acaaggacag gatcaggcac cacagcagga
     6601 tggaaccctc gtggtcgccc caggtgccag tgatcttgta cagcagcggt ttcgtggcgt
     6661 tggaattctc cgcgacgttc agcacggaga agtcatccac caccgcggac cagaccaggc
     6721 acgcgtagga ggtcagcagc gcgatcatct gcccgagcgc cagggccgga gccgaggcca
     6781 tcaggcggga atcccggtca tgcgcgcccc agagcggcag cacggcctgg gctgcggcga
     6841 cagcgagtgc cagggcgagg gcgaaatggc caagctccgg gatcatgcga tggctctccc
     6901 gatgaatggc gcgtgcctgc ctgccatggt gtctgtcccc atccttgcgc cgagtccgcc
     6961 gccacgcgcg atacgggctg ggatcaaagg acccgccgcg gaaagcgggt ggcatgtgcg
     7021 gacccggtga tccgttccgt cgcaacgccg tgtgcgatca gccgggccga tccgcgcgtt
     7081 tgcccatgcg gcggcaatag gccggcgttg caggcaagac aaccacgatc gtggtaatcc
     7141 gttacgggcg cgtcgggcgt ggattggcta accgcaaggg ccgcgggtgc ggctcatgcc
     7201 accccgcgtg acagccgagt cataagcacg ccggccaccc gagtcttcta tacgggcgct
     7261 cggtgtaaag gcgtggatgc ccgcgccacg cgcgggtggt tccgcggtcc ggtattagat
     7321 ggcggcgatg gcgttggaaa cgaggcccgg caaggtttgc cagtcggcga tcagccagta
     7381 gctggcgacc aacgcaacaa tccacgccac cggcacgacg aatgcgcgga aacccaggtt
     7441 cgacggattc acatcacgct tgggactgcg catccaactc ctccgaggca ggcacgggtc
     7501 cagaccatcg gcggcccgtc ttcttacggc agtgaagatc gtcactgatt acggcagttt
     7561 ttggctgagg atgtgcaact atcacggcat tgcggcattg cggtttgcac atatcccggt
     7621 gatcgccgcg cgcgagcggc gcaacgtaca gtgccgcatt cggcgcggca ccgcgcggtg
     7681 cacacgcaaa ggtggcgcag cgccgctaac tgttgccgat aaggataccg cagccgaaca
     7741 ccagcgcgcc gccgaatgcg acctgcatgg cagcggagaa tggcggggtg tccatgtatt
     7801 tccagcgcac ccagctgatc accgccagtt cgatcacgac cacgacaatg gcgatcgcgg
     7861 tggcggtcca gaaatacggg atcaggaagg gcagcgtatg gccgatgccg cccagcgtgg
     7921 tcatcaggcc ggtgatcagt ccgcgcatca gcggtgcgcc gcggcccgtg aggctgccgt
     7981 tgtccgacag cgcctcggcg aagcccatgg agatgccggc gccgatcgaa gcggccatgc
     8041 cgaccaggaa ggcttcccag ctctggttgc tggcgaaggc ggcggcgaac accggcgcca
     8101 gggtggagac cgagccatcc atcaaaccgg cgagcccggg ctggatcacc cgcagcacga
     8161 aggagcggtg cgcggtaagg ttctccttct ccaccacgtc ggccggcagg ttctccgtcc
     8221 gcagggcgtc ggcgcggtgt tcgtgctcca cctcggcgtc ggcgaggtcg ccgagcagct
     8281 tgcggatgga gacgtcctgg ctgcgcgtgg cagcgcggcg gtagaactgc tgggtctcct
     8341 gctccatgct ctccgccagg ttgcgcacct gttcgagttg caggggatgc aactgccaga
     8401 ccggcgggtg gtggatgaag ccacgaatgt cctggcgtcg gatcagcggg atatgctcgc
     8461 cgaacttctc ccggtacagc tccagcaggc ggcggcggtg ttcgccttcc tcggccgcca
     8521 tctcggtgaa cacgttggcg ctgccgggat agtcctgccg caatccctcg gcgatatcga
     8581 ggtagatacg gccgtcctcc tcctcgttgg caattgccag ggcgaggacc tcgcgttcgg
     8641 tcagatcgga gaaattgcgc atggcggcga atgtggcggc tggcgcggga tggtcaagcg
     8701 gcgaacgaaa tgatgcgagt cactcgcagt cagcgtgtgt ggcgtccggc gcgcctcttg
     8761 acgacccagc cacgcctcgg tcacacccgg cacgcaccct gccgcggctt tgctgtccag
     8821 gatcaacaat gcgatccggc agcgagcccc aggcccacgc ccgtccgtcg acaatccgcc
     8881 cagcttccgc ccgccagggg agaaacacgc atgccttcga tcacccgtag aagattcggc
     8941 cttcagatgc tggccggctc cgccgcgctc gccggcacgc gcggcacgcc cgcctatgcg
     9001 gccgacaaag tccatgtcgg cattctgccg ctgacctcgc atgccccgac catcatcgcc
     9061 agcgccaaag ggtatttcgc cgcccagggg ctggaagcca cgttggtcag cttcgaggcg
     9121 gcgcagccga tggcgatcgc catcgcctcc ggcgatgtgg atttcggggt aaccgcgatc
     9181 accggcggcc tgatcagcct ggcggacaag ggcgcggtga aggtgatcgg cggcgcgctg
     9241 caggaatatc cgaacgtcga gggccagaag atccttgcgt ccaaggcggc ctacgacaag
     9301 ggcctgacca gcccggcgca gctgaaggta cacagcttcg gcatcaccac cgccggctcc
     9361 tcctttcagt acatggcgca taagatcgcg cagaagcagg gctggccgga ttcggtcatt
     9421 ccgctgcgtc cgctgcaaag cgtgccgaac gtggtcgcgg cattgaagag cgggcagatc
     9481 gatgcctggt cgatcgtgcc caacatcgcc gacgcgctga ccaagggcgg ggatgtgaag
     9541 cagatcggca agatcgccga tttcatcccg gactatcagg tgaccgtggt gttcacctcc
     9601 accgccaacg tgaccaaacg gccggcgctg gtgaagcgct tcctcgcggc atacgagaag
     9661 ggggcgagcg actacaacgc cgcgctggtg gacaggacga tgggcccgaa ggagacggcc
     9721 gacattgtcg ccatgatcca caaatacgtc tacgcgtccg tgccgctgga caaggcggcg
     9781 ccacggatca agcacggcgc catgcggatc aatcccggcg cgcgcatgaa cctggccagc
     9841 atcaaggacc agctcgactg gttcaaggcg gagcacatgg tcccggtggg cgtcacgatg
     9901 gagcaactgg tgaacacaag cttcgtcaag gcgggctaag gggggagaga cacgagactc
     9961 cccctcccct tgcgggaggg ggttgggggg agggggcggt catgagtacc cggggacagc
    10021 gccagcgccc cctcccccca ccccctcccg caaggggagg gggagttctt tgacggggca
    10081 ccatggaact cagcatcgag aacatcaccc acgcctacga tgcgacaaag gtgctggagg
    10141 acatctcctt caccgtgcgg ccgggggaga tcgtctgcat cgtcggcccc tccggctgcg
    10201 gcaaatcgac cctgctgcgt ttcgttggcg ggctggagcg gccggatgcc ggccgggtga
    10261 tgctgctcgg cgatccgccg gcgggctcgc tgaatccgct gacctacatc ttccaggact
    10321 tcgccctgct gccctggcgc accgtggagg gcaatgtcag cctggtgctg gaggaccatg
    10381 gcatgccgcg cgcccaggcc cgcgccatca tcgacgacgt gctggcccgc accaacctgt
    10441 ccgatttccg caaagcctgg ccccgccagc tctccggcgg catgaagcag cgcgtcgcca
    10501 tcgcccgcgc gctcagcgtc cgcccggcgg tgatgctgat ggacgagccg ctctccgcgc
    10561 tggacagcca gacgcgcgaa ctgctgatgg acgacctggt ggagctgtgg tcccgcgagg
    10621 gtttttccgc ctgctacgtc acccacaacc tcgccgaggc cgtgcggctc ggccacaaga
    10681 tcatcgtgct gtcgcgccgg ccggggcgga tccgggaggt tgtcaccatc gacatgccgc
    10741 tgtccgaacg cccggagcgc ggcgccgaac tcgaccggct gcagcggcag gtctggcaga
    10801 tcatgcgcga ggaagcacgc caggcggaca tggaactgac cgatgctgga tgagccggtg
    10861 caacagcgcc cggcggcggc tgccggaccg ggggagccgg cgcgtccgga ggcgcgggcg
    10921 gtgccgtttc gcggcggcgg cttccgggcc aagcggcagc cgctggtggc ctggctggtg
    10981 ttcgccgcgc tgctgctgct gtgggaagcc ggcagccgga cggggctgat cagcaacatc
    11041 gtgctgcccg ccccctccga ggccttcgcc gccttcatgg acctggtgcg caccggcatg
    11101 ctgtggaagc acctggacgc cagcctgtac cggctgatcg tcggctggac cgccggcacc
    11161 gcgttgggca tcgtcgtcgg gctgctgatc gggctgtcgt cgctggcgcg tgccgggctg
    11221 ctgcaattgg tctcggcgct gttcgccatc ccgaagatcg cgctgctgcc gctgttcgtc
    11281 gtgtggttcg gcatcggcga gggatcgaag atcgccacca tcctgttcgg cacgttcttt
    11341 ccgacagtga tc
//
LOCUS       DASSZH010000166         3725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1572116, whole genome shotgun sequence.
ACCESSION   DASSZH010000166 DASSZH010000000
VERSION     DASSZH010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572116"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VFN42_03620"
     CDS             <1..333
                     /locus_tag="VFN42_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HET8995734.1"
                     /translation="YRRAANILRIEERKDGPHDAAPDQALLREPAEVDLNHALGALPM
                     ILRQLDQEDFTGAMAGLAGLRAPLDSFFDRVTVNAPEPELRRNRLRLLNQVRVTMDRV
                     ADFSRIEG"
     gene            347..3016
                     /gene="ppdK"
                     /locus_tag="VFN42_03625"
     CDS             347..3016
                     /gene="ppdK"
                     /locus_tag="VFN42_03625"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HET8995735.1"
                     /translation="MTKWVYSFGAGHNEGRADMKNLLGGKGANLAEMASIGLPVPPGF
                     TITTEVCTAFYENKRKYPDGLEEEVRQALSRMEQGLGRKFGDAKNPLLVSVRSGARVS
                     MPGMMDTVLNLGLNDETVQGLAAMAGDERFAWDSYRRFIQMYGSVVLGVDHHRFEEII
                     EHAKLETGAVEDTQLKAEDWQRVAAAYKEMIKQDTGKPFPLKPQEQLWGAIGAVFGSW
                     MNPRAITYRRLHDIPAEWGTAVNVQAMVFGNMGNDCATGVCFTRDPSTGENRFYGEYL
                     VNAQGEDVVAGIRTPQPVSSARAKPGETPMEAVMPEAYRELLEVREKLEQHYRDMQDI
                     EFTVQQKKLYMLQTRNGKRTAAASLRMAVDMAHEGLITEAEAVMRVNPSALDQLLHPT
                     LDPQAPRTALAKGLPASPGAASGAVVFNADEAESRAGKGEAVILVRIETSPEDIHGMH
                     AARGILTTRGGMTSHAAVVARGMGRPCVAGAGGITVDYGAQTLSSGGKTVQAGDIITL
                     DGATGEVFVGSVPMVEPAMSGDFATLMGWADKVRRMGVRANAETPLDAETARKFGAEG
                     IGLSRTEHMFFDPERIGAVRQMIMANDEAGRRRALDRLLPFQREDFRKLFTIMAGLPV
                     TIRFLDPPLHEFLPHAEDEMRDVANGMGTDVETIRRRAAELAEANPMLGHRGCRLGIS
                     YPEIYEMQARAIFEGALAVAEETGNPPHPEIMIPLIATRKELEITRAQVDKVAEEVFE
                     KAGRRIEYSVGTMIELPRAVLVADKIAEIADFFSFGNNDLTQTVFGLSRDDAGKFLPQ
                     YVEQGILPKDPFVSIDPEDVGEMVRIGAEKGRKTRGKLKLGICGEHGGDPASIAFCET
                     VGLDYVSCSPYRVPVARLAAAQAALGVEGDRTA"
     gene            3083..3370
                     /locus_tag="VFN42_03630"
     CDS             3083..3370
                     /locus_tag="VFN42_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003630730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HET8995736.1"
                     /translation="MSYGFEWDEAKRRANVAQHGVDFIDAALIFRNPVLEAEDRRGDY
                     GEQRLRALGHVGGDFFMVVFTWRGNNRRIISAWKVDAYGKKRYQAILARGD"
     gene            3321..3611
                     /locus_tag="VFN42_03635"
     CDS             3321..3611
                     /locus_tag="VFN42_03635"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HET8995737.1"
                     /translation="MHMERSDTRRYSLEEIEEQRAQGRSETRHDAPEHAVDDAFWREA
                     RVVMPPGKTSVHLRLDADVLEWFKAQGPGHLTRMNAVLRAFMEAHRRRGSAP"
BASE COUNT          660 a         1202 c         1306 g          557 t
ORIGIN      
        1 tatcggcgcg ccgccaacat cctgcggatc gaggagcgca aggacggtcc gcacgatgcc
       61 gcgccggacc aggccctgct gcgcgagccg gccgaggttg atctgaatca tgcgcttggg
      121 gcgctgccga tgatactgcg ccagctggat caagaggatt tcaccggggc gatggctggc
      181 ctggcggggc tgcgcgcccc attggacagc ttcttcgacc gcgtgaccgt caacgccccg
      241 gaaccggagc tgcggcgtaa tcgtttgcgt ctgcttaacc aggttcgggt taccatggac
      301 cgcgtcgccg atttctcccg tattgaaggt tagaccgagg gttcacatga ccaagtgggt
      361 gtacagcttc ggagccggcc acaatgaggg ccgcgccgac atgaagaatc tgctcggcgg
      421 caagggcgcc aacctggcgg aaatggcctc catcggcctg cccgtgccgc ccggcttcac
      481 catcaccacc gaagtctgca ccgccttcta cgagaacaag cggaaatatc cggacgggct
      541 ggaggaggag gtccgccagg cgctgtcccg gatggaacaa ggcctcggcc gcaagttcgg
      601 tgacgcaaag aacccgctgc tggtgtcggt gcgctccggc gcgcgcgtgt ccatgccggg
      661 catgatggac accgtgctga accttggtct gaacgacgag accgtgcagg gcctcgccgc
      721 catggccggg gatgagcgct tcgcctggga ttcctatcgc cgcttcatcc agatgtacgg
      781 ctccgtcgtg ctgggggtgg atcaccaccg cttcgaggag atcattgagc acgccaagct
      841 ggagaccggc gcggtcgaag acacccagtt gaaggcggaa gactggcagc gcgtcgccgc
      901 cgcctacaag gagatgatca agcaggacac cggcaagcca tttccgctga agccgcagga
      961 acagctttgg ggcgccatcg gcgcggtgtt cggctcctgg atgaacccgc gcgcgatcac
     1021 ctatcgccgg ctgcacgata tccccgccga atggggcacc gccgtgaatg tgcaggcgat
     1081 ggtgttcggc aacatgggca atgactgcgc caccggcgtc tgcttcaccc gcgatccgtc
     1141 caccggggag aaccgcttct acggcgagta cctggtcaac gcgcagggcg aggatgtcgt
     1201 cgccggcatc cgcacgccgc agccggtgtc gtccgcgcgg gccaagccgg gcgagacgcc
     1261 gatggaagcg gtgatgccgg aggcgtaccg cgaactgctg gaagtgcggg agaagctgga
     1321 acagcactac cgcgacatgc aggacatcga gttcaccgtg cagcagaaga agctgtacat
     1381 gctgcagacg cgcaacggca aacgcaccgc cgcggccagc ctgcgcatgg cggtggacat
     1441 ggcgcatgag ggcctcatca ccgaggccga agcggtgatg cgggtgaacc cctcggcgct
     1501 cgaccagttg ctgcatccca cgctggaccc gcaggcgccg cgcacggcgc tggccaaggg
     1561 cctgcccgcc agccccgggg ccgcatcggg tgcggtggtg ttcaacgccg acgaggcgga
     1621 aagccgcgcc ggcaagggcg aagcggtgat cctggtgcga atcgagacca gcccggaaga
     1681 catccacggc atgcacgccg cccgcggcat cctgaccacc cgcggcggca tgaccagcca
     1741 cgccgcggtg gtggcgcgcg gcatgggccg gccctgcgtc gccggcgccg gcggcatcac
     1801 ggtggattac ggggcgcaga ccctgtcctc cggcggcaag accgtgcagg cgggcgacat
     1861 catcaccctg gacggcgcga ccggtgaggt gttcgtcggc tccgtgccga tggtggagcc
     1921 ggccatgtcc ggcgacttcg ccacgctgat gggctgggcc gacaaggtcc gccgcatggg
     1981 cgtgcgcgcc aatgccgaga cgccgctgga cgccgagacc gcgcgcaagt tcggcgccga
     2041 aggcatcggc ctgtcgcgca ccgagcacat gttcttcgac cccgagcgca tcggcgcggt
     2101 gcgccagatg atcatggcca atgatgaggc tgggcggcgc cgcgcgctgg atcggctgct
     2161 gccgttccag cgcgaggact tccgcaagct gttcaccatc atggccgggc tgccggtgac
     2221 gatccgcttc ctcgacccgc cgctgcacga attcctgccg catgccgagg acgagatgcg
     2281 cgacgtggcg aacggcatgg gcaccgacgt ggagaccatc cgccgccgcg ccgcggaact
     2341 ggcggaagcc aacccgatgc tggggcatcg cggctgccgg ctggggattt cctatccgga
     2401 gatctacgag atgcaggcgc gcgccatctt cgaaggcgcg ctggcggtgg ccgaggaaac
     2461 cggcaatccg ccgcatccgg agatcatgat cccgctgatc gccacccgga aggagctgga
     2521 gatcacccgc gcccaggtgg acaaggtggc ggaggaggtg ttcgagaaag ccggccgccg
     2581 gatcgagtac agcgtcggca cgatgatcga gctgccgcgc gccgtgctgg tggccgacaa
     2641 gatcgccgag atcgccgact tcttcagctt cggcaacaac gacctgacgc agaccgtgtt
     2701 tggcctgtcg cgcgacgacg cgggcaagtt cctgccgcaa tatgtcgagc agggcatcct
     2761 gccgaaggac ccgttcgtgt cgatcgaccc ggaggacgtc ggcgagatgg tgcgcatcgg
     2821 cgccgagaag ggccgcaaga cccgcggcaa gctgaagctg ggcatctgcg gcgagcacgg
     2881 cggcgatccg gcctcgatcg cgttctgcga gacggtgggg ctggattacg tgtcgtgcag
     2941 cccgtaccgc gtgccggtgg cgcggctggc ggcggcgcag gcggcgctgg gggtcgaagg
     3001 cgaccgcacc gcgtaggttc caggccgccc gtgagcctgg aagcttgcgg gcgcttgccc
     3061 ttgtggacac atcggtgtac ccatgtccta cggtttcgag tgggacgagg ccaagcggcg
     3121 cgccaatgtg gcccagcacg gcgtggattt catcgacgcg gcgctgatct tccgtaatcc
     3181 cgtactcgag gccgaggacc ggcgcggcga ttacggcgag cagcgcctac gcgcattggg
     3241 ccacgtgggc ggcgacttct tcatggtcgt gttcacctgg cgcgggaaca accgccggat
     3301 catcagcgcc tggaaggtcg atgcatatgg aaagaagcga taccaggcga tactcgctcg
     3361 aggagattga ggagcaacgc gcgcagggtc ggagtgaaac gcggcacgac gctccagagc
     3421 acgctgtcga tgacgcattc tggcgcgaag ctcgggtggt gatgccaccc ggcaagacgt
     3481 cggtgcatct ccggctcgat gccgatgttc tggagtggtt caaggcgcag ggcccgggtc
     3541 atctgacgcg catgaatgcc gtgctgcgcg ccttcatgga agcacaccgg cgacgtgggt
     3601 ctgccccata gccgcagtca cgttcgactc cgcgaccgtg atccacgaca gcgacgaagc
     3661 gcgagcgccg cagcgtcgcg cacaccgcaa acggcgccga acatttccgc ggtgaaccgc
     3721 gtatc
//
LOCUS       DASSZH010000167         2860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_276748, whole genome shotgun sequence.
ACCESSION   DASSZH010000167 DASSZH010000000
VERSION     DASSZH010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276748"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..442
                     /locus_tag="VFN42_03640"
     CDS             56..442
                     /locus_tag="VFN42_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET8995738.1"
                     /translation="MDIRSSKDMVAQADAAVRTLGPEQAVKLAGDPNTVFVDVRESGE
                     VQKSGTLSGAVHAPRGFLEFMADPASPRHLSELSPGKQVVVFCGSGQRSALAARTLKE
                     MGYPNVAHVAGGFGALKQAGGEISGG"
     gene            complement(666..1976)
                     /locus_tag="VFN42_03645"
     CDS             complement(666..1976)
                     /locus_tag="VFN42_03645"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HET8995739.1"
                     /translation="MRIAMIGGGYVGLVSAACFAEFGIEVTVVEADAQKLAALHEGRM
                     PIYEPGLEKLVAENVAACRLSFTGELPPAVAGVDAVFIAVGTPTRRGDGHADLRYVFA
                     AAEQVGRALTGYAVIVTKSTVPVGTSRRIAEIVRAARPELEFDVASNPEFLREGSAIG
                     DFMRPDRVVIGAESDRAREVLRTLYRPLYLIEAPIVFTNIETAELTKYAANAFLAMKV
                     TFINEMADLCEKVGADVHDVARGIGLDGRIGRKFLHPGPGYGGSCFPKDTLALVRTAQ
                     DYGAPSRLVEATVAVNDARKSNMAMRVIAACGGSVRGKTIAVLGLTFKPETDDMRDAP
                     AISIVARLAGDGATIRAFDPEGMEQARTVLPDSVEYCRDALDAATGADALVLITEWNE
                     FRALSPVRLREAMRGQVLVDLRNVYEPAAMRQAGFAYHGIGRAG"
     gene            complement(2138..2569)
                     /locus_tag="VFN42_03650"
     CDS             complement(2138..2569)
                     /locus_tag="VFN42_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HET8995740.1"
                     /translation="MSQLTLDQAQTIVSVALQHARAQKFQPLAVIVLDARGVLKAAGV
                     EDGTSLRRSEIATGKAHGALSFGVGSRNLARRAEERPHFIAAVTHAVGGMLVPVPGGV
                     LIRAADRSVLGAVGVSGDNSDNDEAAAMAGIAAAGLTGDPG"
     gene            complement(2573..>2860)
                     /locus_tag="VFN42_03655"
     CDS             complement(2573..>2860)
                     /locus_tag="VFN42_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8995741.1"
                     /translation="GGPAVFFGDIAFYSGDWDARERVGRIDTNRCRLFMLTGEYDYSC
                     TVEHSQATAAKIPGVRFQAMQGIGHFPFAENPGLFAEYLLPILDEVHDQKD"
BASE COUNT          472 a         1004 c          955 g          429 t
ORIGIN      
        1 gccggcggcc cgcatgccgg cgattcagac cagcagcagg agaggagtca ggacaatgga
       61 tattcgcagt tccaaggaca tggttgcaca ggcagatgcc gccgttcgaa ccctcggtcc
      121 ggaacaggcg gtgaagctcg ccggcgatcc gaatacggtg ttcgtcgatg tgcgggaaag
      181 cggcgaggta cagaaatccg gcacgctgag tggcgcagtg catgcaccgc gcggcttcct
      241 ggaattcatg gcggacccgg ccagcccgcg gcatctgtcg gagctctcgc ccggcaagca
      301 agtggtggtg ttctgcggct ctggccaacg ctccgcactg gcggcgcgca cactgaagga
      361 aatgggctat ccgaacgtcg cacacgtggc cggcgggttc ggcgcgctga agcaggccgg
      421 cggggagatc agcggcggct gatccggctt cattgcgccg gcgtcgtggc gaaggcgcga
      481 cacccatgac ttgcctcagg agacccagcg ccaccagcgg ctttggcgtt tcctacagca
      541 agacatggat ggcgagcctt cgcctgccac gacggagaag gcactggggt tcgcggccat
      601 taccggcagc cgcccccatg accagcacgc gcggccgtga cacagcgggc gccctgccgg
      661 tgcggttatc ccgcgcgccc gatgccgtga tacgcgaatc ccgcctgccg catcgccgcg
      721 ggctcgtaca cattgcgcag gtccaccagc acctggccgc gcatcgcctc gcgtagccga
      781 accggcgaca gggcgcggaa ctcgttccat tcggtgatca gcaccagcgc gtccgctccc
      841 gtcgccgcgt ccagcgcatc gcggcagtac tcgacgctgt ccggcagtac cgtgcgcgcc
      901 tgttccatgc cctccggatc gaaggcgcgg atcgtcgccc cgtcccccgc caagcgcgcc
      961 acgatcgaaa tcgccggtgc gtcgcgcatg tcgtcggttt ccggcttgaa ggtcaggccg
     1021 agcaccgcga tggtcttgcc gcgcaccgag ccgccgcagg ccgcgatcac ccgcatggcc
     1081 atgttcgact tgcgcgcgtc gttgaccgcg accgtcgcct ccaccagccg gctcggcgcg
     1141 ccgtaatcct gcgcggtgcg caccagggcg agcgtgtcct tcgggaagca ggacccacca
     1201 taacccgggc cggggtgcag gaacttgcgg ccgatgcgtc cgtccaggcc gatgccccgc
     1261 gccacgtcat gcacgtcggc gccgaccttc tcgcacagat ccgccatctc gttgatgaag
     1321 gtgaccttca tcgccaggaa agcattggcg gcatatttcg tcagctccgc cgtctcgata
     1381 ttggtgaaca cgatcggcgc ttcgatcagg tagagcggcc ggtacagcgt gcgcagcacc
     1441 tcgcgcgcgc ggtccgattc ggcgccgatc accacccggt caggccgcat gaaatcgccg
     1501 atcgcgctgc cctcgcgcag gaactccgga ttggaggcaa cgtcgaattc cagctccgga
     1561 cgagcggcgc ggacgatctc cgcgatacgc cggctggtgc cgaccggcac ggtggacttg
     1621 gtgacgatca ccgcatagcc ggtcagcgcc cgccccacct gctctgccgc ggcaaagacg
     1681 tagcgcaggt cggcgtggcc atcgccgcgg cgggtggggg tgccgacggc gatgaacacc
     1741 gcgtccacgc ccgccacggc cggcggcagt tcgccggtga aggacagccg gcaggcggcg
     1801 acgttctccg ccactagctt ttccaggccg ggctcgtaga tcggcatccg gccctcatgc
     1861 agggcggcca gtttctgcgc gtcggcttcg acgaccgtga cctcgatccc gaattcggca
     1921 aagcaggcgg cggagaccag gccgacatag ccgccgccga tcatggcaat acgcacggat
     1981 gaagcatcct tcgacaggag gagcggggga ttcagcacag cagcggatcg agttccagcg
     2041 aattgactgc gacagcccgt gcccccgccc gatcggccgg gccgagccgc cggtcagcct
     2101 gcgccggagc ggtttccccc gggttaatgc accacggtca gcccgggtcg ccggtcaggc
     2161 cggccgcggc gatgcccgcc atggccgcgg cctcgtcatt gtccgaattg tcgccggaca
     2221 cgccaacggc gcccagcacg ctgcggtccg ccgcgcggat cagcaccccg cccggcaccg
     2281 gcaccagcat gccgcccacc gcatgggtga ccgcggcgat gaaatgcggc cgctcctccg
     2341 cccgccgggc caggttgcgg gagccgacgc cgaaggacag cgcgccgtgc gccttgccgg
     2401 tggcgatctc cgaccggcgc aggctggtgc cgtcctccac gcccgccgcc ttcagcacgc
     2461 cacgcgcatc cagcacgatc accgcgagcg gttggaactt ctgcgcccgc gcgtgctgca
     2521 gggcgacact gacgatggtc tgggcctggt cgagcgtgag ctgggacatg ggtcagtcct
     2581 tctgatcatg aacttcgtcg aggatcggca gcaggtattc ggcaaacagg ccgggatttt
     2641 cggcaaacgg gaaatgcccg atcccctgca tcgcctggaa gcggacgccg ggaattttcg
     2701 ccgcggtcgc ctgggagtgc tccaccgtgc aggagtagtc gtattcgccg gtcagcatga
     2761 acagccggca gcggttggtg tcgatgcgtc cgacgcgctc gcgcgcatcc caatcgccgg
     2821 aatagaaggc gatatcgccg aagaacaccg ccggtccgcc
//
LOCUS       DASSZH010000168         3325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_838918, whole genome shotgun sequence.
ACCESSION   DASSZH010000168 DASSZH010000000
VERSION     DASSZH010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838918"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="VFN42_03660"
     CDS             complement(<1..1138)
                     /locus_tag="VFN42_03660"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8995742.1"
                     /translation="MFRSLHLLRQLPLTARLLIACRVARSVGQGALVTDFALYLGALH
                     WDAVQMGAVYMGGLTIGAMLTMAAGPLSDSVGRKPFLIGYEIAQLLAAGLALATAQPT
                     WLVLASMVGAYGRGANGAAGPFGPVEQAWLSDCIPEGDFGAVYSLNAAAGFGGMAVGS
                     MLAALPALWSGVLPGPLRFRPLFLLVLLGSLLSLALLTRMRETRGAARHPAEQAPSRA
                     AEPPRGRLMLAKLVGINTLNGLAIGVIGPFMAFWFHLRYGAGPAAIGPVLALGFVLAS
                     FASVWAGWLSRRLGTTEAVVVMRLAGLVLLVALPFMPSFGLAAACYVLRSAFNRGTAG
                     ARQAVGLLLVGRDRRGLAASLNAISMQIPRALGPVLGGWLLDTQL"
     gene            1310..1621
                     /locus_tag="VFN42_03665"
     CDS             1310..1621
                     /locus_tag="VFN42_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995743.1"
                     /translation="MGKQSQDREHRVDEASEESFPASDPPSFTPGTGVGDNREDDRQG
                     QQRRAVAAERVERQRDPHGIAPAQQADQGEPAARPTSDRHRKETTVERIAKNRSPSTP
                     G"
     gene            complement(1683..2567)
                     /locus_tag="VFN42_03670"
     CDS             complement(1683..2567)
                     /locus_tag="VFN42_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995744.1"
                     /translation="MSPPAMATLLRAAGDDNFITLGDGNHVVLASGTINRIVAGTATG
                     TNDTNYVVTGGNSTVILGAGHNTVVATGDTSAISTGDGVQHITALGEHNSVTIGSTPV
                     RGEEHHAGFDRFDDDHGGGGSVIQTGDFATISAGDGNTSITATGGFDLVRVGNGDDSI
                     VLLDGTNAGVSTVFLGNGNDRLFLTGEHNTVFGGSGSDTIVDGGIGSDTFILSAAGGS
                     TTIDNFQSTDHLVLSQLTGGNLSDLTLTTVADPRYGSGAVDTLITATAAGGTAQVTLH
                     NFDGVSLAAMMAAQSLYT"
     gene            complement(2521..2808)
                     /locus_tag="VFN42_03675"
     CDS             complement(2521..2808)
                     /locus_tag="VFN42_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995745.1"
                     /translation="MAYDHDGKHHGWFTDTIYSTADGAVITEGDGNYNATIIGNGSTL
                     VVGNGNQFVTVSGTGDQITIGNSPLMSWISSTTGNVNVTAGDGNVAAGGRR"
     gene            3023..3160
                     /locus_tag="VFN42_03680"
     CDS             3023..3160
                     /locus_tag="VFN42_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995746.1"
                     /translation="MARQRAATRLLDYAITVFQSSDPSGAALGGQIRDPPPMRRARAT
                     A"
BASE COUNT          562 a         1149 c         1131 g          483 t
ORIGIN      
        1 gaagctgggt atccagcagc cagccaccga gcactggccc cagcgcgcgc gggatctgca
       61 tggatatcgc gttgaggctt gccgcgaggc cgcgccggtc gcgtcccacc agcagcagac
      121 ccaccgcctg ccgtgcgcca gccgtgccgc ggttgaacgc cgaccgcagc acatagcacg
      181 ccgccgccag cccgaagctt ggcatgaagg gcagcgcgac cagcagcacc agcccggcca
      241 ggcgcatcac caccactgct tcggtggtgc ccagccggcg ggacagccag ccggcccaga
      301 ccgaggcgaa gctcgccagc acgaagccca gggcgagcac cggcccgatg gcggccggac
      361 cggcgccgta gcggagatga aaccagaagg ccatgaaggg cccgatcacc ccgattgcca
      421 gaccgttcag cgtgttgatc cccaccagtt tggcaagcat cagccggccc cgcgggggtt
      481 ccgccgcgcg gcttggcgcc tgctctgccg gatggcgcgc ggcaccgcgc gtctcacgca
      541 tgcgtgtcag cagggcgagg ctgagcaggc tgccgagcag caccagcagg aacagcggcc
      601 gaaagcgcag cggaccaggc agcacgccgc tccatagcgc gggcagggcg gccagcatcg
      661 agccgaccgc catgccgccg aacccggcgg cggcgttcag gctgtagacc gcgccaaaat
      721 cgccctccgg gatgcagtcg gagagccagg cctgctccac cggaccgaac gggccggccg
      781 ccccgttggc gccgcggcca taggcgccga ccatcgacgc cagcaccagc caggtcggct
      841 gggcggtggc cagcgccagc ccggcggcga gcaactgggc gatctcgtag ccgatcagga
      901 acggcttgcg gccaaccgag tcgctcagcg gcccggcggc catggtcagc atcgcgccga
      961 tggtcagccc gcccatgtac acggcgccca tctgcaccgc gtcccagtgc agcgccccca
     1021 ggtacagcgc gaaatcggtc accagtgcac cctggccgac gctacgcgcg acccggcatg
     1081 cgatcagcaa tcgggcggtg agcggcagtt gccgcagcag atggaggctc cggaacaccg
     1141 ctccggcggc tcggggcggc gagacaggcg tcatggcggg caggcgagca caggagcccg
     1201 ggcggaacaa gccgcgtcgc tgcaacgctc atttggcacc cacgcagccc tggccgacgc
     1261 actctgggcg cggtgctgcg ttctgtccgg atgaccgaag gagggttcga tgggtaagca
     1321 gagccaggat cgcgagcatc gcgtcgatga ggcgagcgag gagagctttc cagcctccga
     1381 cccgcccagc ttcacgccgg ggaccggggt tggcgataac cgtgaggacg acagacaggg
     1441 ccagcagcgc cgggccgtgg cggccgagcg ggtggagcgg cagcgcgatc cgcatggcat
     1501 cgccccggcg cagcaggcgg accagggcga gccggcggca aggccgacat ccgatcgcca
     1561 tcgcaaggag accacggtcg aacggatcgc caagaaccgc tcgccgtcga cgccaggctg
     1621 atccgatccc gggtgaacac gaacgcgccg cgcggttcgt ccgtggttgg atacgtgatc
     1681 ccttacgtgt acagcgactg ggcagccatc atcgccgcga gcgacacgcc gtcgaaattg
     1741 tgcagcgtca cctgtgcggt gcctccggcg gcggtggcgg tgatcagcgt gtccaccgca
     1801 ccgctgccgt aacgcggatc agccacggtg gtcagggtca gatcgctcag attgccgccg
     1861 gtcagttgcg acagcaccag gtggtcggtg gactggaagt tgtcgatggt ggtcgatccg
     1921 ccggcggcgc tcaggatgaa ggtgtcgctg ccgataccgc catcgacaat ggtgtcggag
     1981 ccgctgccgc cgaacaccgt gttgtgctcg ccggtcagga acagccggtc attgccgttg
     2041 cccaggaaaa cggtgctgac cccggcattc gttccatcga gcaagacgat ggagtcatcg
     2101 ccgttgccca cgcggacgag gtcaaaaccg ccggttgcgg tgatgctggt gttgccgtcg
     2161 ccggcgctga tcgtggcgaa gtctccggtc tggatcacgc tgccgccgcc gccatgatcg
     2221 tcatcgaacc tgtcgaagcc ggcgtggtgt tcctcaccgc gcacgggcgt gctgccgatc
     2281 gtgacgctgt tgtgctcgcc gagggcagtg atgtgctgca cgccatcgcc ggtgctgatg
     2341 gcgctggtat cgccggtggc caccaccgtg ttatggccgg cacccagaat gacggtggag
     2401 ttgccgccgg tgaccacata gttcgtgtcg ttcgtcccgg ttgccgtgcc ggccacaatg
     2461 cggttgatgg taccggacgc cagtaccacg tggttgccgt caccgagcgt gatgaaattg
     2521 tcatcgccgg ccgcccgcag caacgttgcc atcgccggcg gtgacattca cgttgcccgt
     2581 ggtgctgctt atccagctca tcagcggact attgccgatg gtgatctgat cgcctgtccc
     2641 ggaaaccgtc acgaactggt tgccgtttcc caccaccagc gtggagccgt tgccaatgat
     2701 ggtggcgttg tagttgccgt cgccctcggt gatgacggcg ccatccgccg tgctgtagat
     2761 cgtgtcggtg aaccagccgt gatgcttgcc gtcgtgatcg tacgccatcg ccttcctcct
     2821 gggactggac gccggcggca aaccccgaac cgcgccagcg tcgatcggct tgttgcaaca
     2881 ccacaatggg aatggctgga ttgcgcccgc gctacacgaa tttggccggc ccaggccgct
     2941 ctcaggtgct gcgtcccgag ccgcgcttgc tccggtaacg cggttcggca acaaaattca
     3001 cgcggcgcgc atgctccatg tcatggccag gcagcgggcc gcgacccgtc tgctcgacta
     3061 tgccatcacc gtgttccaga gcagcgatcc gagcggcgcc gcgctggggg gacagatccg
     3121 cgacccgccg ccaatgcgcc gggcgcgggc gaccgcatga cggcaatgca gttgctggaa
     3181 caggcgatca ccagggccgg caatcgggtg acgccgcagt tcacccctcc agccgcagtc
     3241 cgaaccgctg cgcaaacccc tcccgcccgg ccggggtgat cgccaccgcg cgcgtgtcgc
     3301 gcagccgttc cacccagccc agcgc
//
LOCUS       DASSZH010000169         3044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_634671, whole genome shotgun sequence.
ACCESSION   DASSZH010000169 DASSZH010000000
VERSION     DASSZH010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634671"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VFN42_03685"
     CDS             <1..471
                     /locus_tag="VFN42_03685"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET8995747.1"
                     /translation="VGEFLLAGDTFGFDDIGSYDLAAEAVSDVVLRRYPRRMVESLAE
                     RHSGLAQRLRTMALTSLRTAHDRALSLGRKTAVERIASFLLEMERRSAPHDEPLLELP
                     MCRTDIADHLGLTVETVCRTLAFLKREGLIGITRTGVELRVRPALRDLACEARH"
     gene            complement(481..1065)
                     /locus_tag="VFN42_03690"
     CDS             complement(481..1065)
                     /locus_tag="VFN42_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007397139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HET8995748.1"
                     /translation="MSAIHSVAVFCGSRAGDDPAFAHAARLLGRGMAEAGIRLVYGGG
                     RVGLMGAVADAVLAAGGEVLGVIPEFLTRLEVAHSGISEMVVTDSMHSRKQRMFAESD
                     AFVSMPGGLGTLDETIEIITWRQLGLHDKPILVCDVAGSAGPLVGAIEGAIANGFARP
                     DILRLFELVHGVPALLSRLRELPAAAAAVPAERL"
     gene            1189..1626
                     /locus_tag="VFN42_03695"
     CDS             1189..1626
                     /locus_tag="VFN42_03695"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET8995749.1"
                     /translation="MRRTLALAVIASLAVAGLASAAEPDPIAVRQAGQDLLFGNFTGM
                     RTTAAANGDITKMKEPAKAIARWMKVFPTEFPPGSDKGENTKALPAIWSDHAGFVKAA
                     DRLAAAADTLAGYAASRNREKFTAQLKVVGEACAACHKKYKAR"
     gene            complement(1627..2250)
                     /locus_tag="VFN42_03700"
     CDS             complement(1627..2250)
                     /locus_tag="VFN42_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET8995750.1"
                     /translation="MLWPSLPGQSVLGVGFAEPYLRLWQGQATRCLAVTPAQMGATRW
                     PVGAPNLSCTAEEDSLPFPDLSFDRVLLVHGLEQADNARRMLREVWRVLKDDGVLLVV
                     VPNRTGMWAYLETTPFGHGQPYSFGQLGRLLASSLFRVERRDVALYVPPVRWRLLLRG
                     APLMERAGRRMLPGLAGVTLTEAVKDVYAAMPVRSKPRRRLVLADAA"
     gene            complement(2436..>3044)
                     /locus_tag="VFN42_03705"
     CDS             complement(2436..>3044)
                     /locus_tag="VFN42_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995751.1"
                     /translation="AYHALQAAGGDARVHGLILVNLQRFVWREGDPSDIVRRSALRPN
                     AFYLRSIFSLQAWYRLIRADFDVANLLLVFSARLLRRAIASLDPVLSVVAGGLTRVGR
                     VRRSVRTLSGRGVPMLYVLGRNDPGVEELAEYFGRDGWRLRRQGNVTYRVLEDADHTL
                     GAWRVRAALITAIRDWCRSVWGGVPTPEMAAERAEAIQIAAD"
BASE COUNT          543 a         1108 c          987 g          406 t
ORIGIN      
        1 gtgggcgagt tcctgttggc cggcgatacc ttcggcttcg atgacatcgg cagctacgac
       61 ctggccgcgg aggcggtcag cgacgtcgtg ttgcgccgct atccccgccg catggtggag
      121 tcgctggccg aacgccactc cggcctggcg caacggctgc ggacgatggc gctgaccagc
      181 ctgcgcaccg cgcatgaccg cgcgttgagc ctgggacgca agaccgcggt ggaacggatc
      241 gccagcttcc tgctggaaat ggagcgtcgc agcgccccgc atgacgagcc gctgctggaa
      301 ctgccgatgt gccgcaccga catcgccgac cacctcggcc tgacggtgga gacggtctgc
      361 cgcaccctgg ccttcctgaa gcgcgagggc ctgatcggca tcacccgcac cggcgtggaa
      421 ctgcgcgtcc gtccggcgct gcgcgacctg gcctgcgaag cccgtcactg accaaccggg
      481 ctacagccgc tcggctggaa ccgcggcggc ggcggccggc agctcccgca accggctcag
      541 cagcgccggc acgccgtgca ccaactcgaa cagccgcagg atgtccggcc gagcgaagcc
      601 attggcgatg gcgccctcga tggcgccgac cagcggcccg gcggatcccg ccacgtcgca
      661 caccaggatc ggcttgtcgt gcagcccgag ctgccgccag gtgatgatct cgatcgtctc
      721 gtccagcgtg cccagtcccc ccggcatcga cacgaaggcg tccgactcgg cgaacatccg
      781 ctgcttgcgg ctgtgcatgc tgtcggtcac caccatctcg ctgatgccgc tatgcgcgac
      841 ttccaggcgg gtgaggaatt ccggaatcac ccccagcacc tcgccgccgg cggccagcac
      901 cgcatcggcc accgcgccca tcagcccgac ccgcccgccg ccatagacca gccggatgcc
      961 ggcttcggcc atgccgcgcc ccaacagtcg ggcggcatgg gcaaacgcag gatcgtctcc
     1021 ggcccgtgag ccgcagaaaa ccgcgacgga atgaatcgcc gacataatcg tccttccaaa
     1081 tgcgaaccgg attggagccg gccgcgtggt ctgatactga ttcctcgtgg ccgggccgag
     1141 tcactgcaac cgcggtcccg cacacgtgac aaaggggaag aggctcgcat gagaagaacg
     1201 ctggcattgg ccgtgattgc aagccttgcc gtggcagggc tggcgagcgc cgcggaaccg
     1261 gacccgatcg cggtccgcca ggcggggcag gacctgttgt tcggcaattt caccggaatg
     1321 cggaccaccg cggccgccaa cggtgacatc accaagatga aagagcccgc caaggcaatc
     1381 gcgcgctgga tgaaggtctt cccgaccgag ttcccgccgg gcagcgacaa gggtgagaac
     1441 accaaggcgc tgccggcgat ctggtcggat cacgccggct tcgtgaaggc ggccgatcgg
     1501 ctcgcggcgg cagccgatac gctggccggg tatgccgcgt cccgcaaccg ggagaaattc
     1561 acggcgcaac tgaaggtggt cggtgaagcc tgtgccgcct gccacaagaa atacaaggcc
     1621 cgctgatcag gcggcatcgg ccaacaccag gcgccggcgc ggtttgctcc gcaccggcat
     1681 ggccgcgtag acgtccttga ccgcctcggt cagcgtcacg cccgccagcc ctggcaacat
     1741 gcggcgaccg gcgcgttcca tcaggggcgc gccgcgcagc agcaagcgcc agcgtaccgg
     1801 cggcacatac agcgccacat cgcggcgctc cacccggaac agcgaactcg ccagcagccg
     1861 gcccaactgg ccgaaggaat acggctgccc gtggccgaag ggcgtggtct ccagataggc
     1921 ccacatcccc gtgcgattgg gcaccaccac cagcaacacc ccatcgtcct tcaacacgcg
     1981 ccagacctcg cgcagcatgc gccgggcgtt gtcggcctgt tccaggccat gcaccagcag
     2041 cacccggtca aaactgagat cggggaaggg gaggctgtct tcctcggccg tacaggacag
     2101 gttcggcgca cccaccggcc agcgcgtggc ccccatttgc gcgggggtca ccgcgaggca
     2161 gcgcgtcgcc tgaccttgcc acagccgcag atacggttcc gcgaagccca cccccagcac
     2221 cgattggccg ggcagactgg gccaaagcaa gatcaaccgt tcgcgtagca gccttgctgc
     2281 tacggcgccg cgcgcggatg cgtagaactc cgccgccgca tgcgaatcag ccgccatgcc
     2341 gggcaatata gcacaatttg ccggcgatgc ccgcccggtc cggatatttc cgggcgaagc
     2401 acggggttgc gaagcggccg ccgggggcgg tggggctaat cggcggcgat ctggatcgcc
     2461 tcggctcgct cggccgccat ttccggcgtg ggcacgccgc cccataccga acggcaccag
     2521 tcccggatcg cggtgatcaa cgcggcccgg acccgccatg cacccaacgt atggtcggcg
     2581 tcctccagca cccggtaggt gacgttcccc tggcggcgca ggcgccagcc atcccggccg
     2641 aaatactccg ccagttcttc caccccgggg tcattgcggc cgagcacgta cagcatggga
     2701 acgccgcgcc cactcagcgt gcggacggaa cgccgcactc gtcccacccg ggtcagccca
     2761 ccagcgacca ccgacaatac cgggtccagc gacgcaatcg cccgccgcag cagccgcgcg
     2821 gagaacacca gcaacaggtt ggccacgtcg aaatccgccc ggatcagccg gtaccaggcc
     2881 tgcaggctga aaatgctgcg cagatagaac gcgttcggcc gcagcgcgct gcgccgcacg
     2941 atgtcggacg gatcgccctc gcgccagacg aagcgctgga ggttgaccag gatcaggcca
     3001 tgcacccgcg catcgccgcc ggccgcttgc agcgcgtgat aggc
//
LOCUS       DASSZH010000170         2563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_617803, whole genome shotgun sequence.
ACCESSION   DASSZH010000170 DASSZH010000000
VERSION     DASSZH010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617803"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..853
                     /locus_tag="VFN42_03710"
     CDS             74..853
                     /locus_tag="VFN42_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HET8995752.1"
                     /translation="MPVEDTWTVAGRRFRSRLIVGTGKYKDLEETAAAIEASGAEIVT
                     VAVRRVNLSDPKAPMLQDFVGPKKITYLPNTAGCHTANEAVRTLRLAREAGGWNLVKL
                     EVLGPPPLLYPDMRGTFEAAEALIKDGFEVMVYCADDPLAAKRLEEMGCVAIMPLGAP
                     IGSGLGVQNPAMLTMMMEQVSVPFLVDAGVGTASDAAIAMELGCDAVLLNSAIALAKD
                     PIKMARAMKLAVEAGRLSYLAGRMPRRMGADPSSPLTGLIR"
     gene            complement(878..>2563)
                     /locus_tag="VFN42_03715"
     CDS             complement(878..>2563)
                     /locus_tag="VFN42_03715"
                     /inference="COORDINATES: protein motif:HMM:NF015372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotide-diphospho-sugar transferase"
                     /protein_id="HET8995753.1"
                     /translation="AIAAIARSTTAVMLQYPFLPPLLAAAHDLAPDGPVPVVFANGAF
                     IPLLENWIAHAHRAGVRRVLVVALDPAVTAHPMRDGCVAVAVPQPGDRAALWQVRLDV
                     FQALALHGIDFIHSDIDAVWLRDPRADFFADPSLDLVFSQGTYHPEQAFTAWNFVLCC
                     GVFAVRAGPRAVEFLEAVHARTPSEIDDQGALNRLLLEHGVQWDTSAAEPYPVPFAGR
                     NFTAYRHLVRSVNTPYGLRFGLLPHHLAARVPSAAPGPLLKHPSSPPDVAGKICVLQA
                     CNCWIAAPRKRPQALVFSYHKSGTTLFDRVMRQVAEQFGLRLVQQYGMAYEIDPAADI
                     VLLPHSLLGFRLARDYRGIRLIRDPRDIWVSGYLYHRRTQEHWCTNTDLDPRPPITYP
                     RVDFSMQHRPERWKRKWLERLNGKSYQQNLLDRDQAAGLEFELQGYTACTLEAMRAWR
                     QDPAVLDIRLEELAGNFDATMRMVFQHFGFGPEECDAAVTLAAREDVARMDDAALAAN
                     THIHSRTLSKWRTVLSPGQVRAFEQRHGDLVRSLGYDLATTADRLGQAQDPAA"
BASE COUNT          460 a          824 c          875 g          404 t
ORIGIN      
        1 aaacctggct ggccagcggc gatgcgctgg agatcgtgca tttcatcggt ggaggctgaa
       61 catggatgga tctatgcccg tcgaggatac ctggacggtg gccggccgcc ggttccgctc
      121 ccggctgatc gtcggcaccg gcaagtataa ggacctggag gagaccgcgg cggccatcga
      181 ggccagcggc gccgagatcg ttacggtcgc ggtgcgccgg gtcaacctgt ccgaccccaa
      241 ggcgccgatg ctgcaggact tcgtcggtcc gaagaagatc acctacctgc ccaacacggc
      301 cggctgccac actgccaacg aagcggtgcg tactctccgc ctggcgcgcg aggcgggcgg
      361 ctggaacctg gtgaagctgg aagtgctggg cccgccgccc ctgctctatc ccgacatgcg
      421 cggcacgttc gaggcggcgg aggcgctgat caaggacggg ttcgaggtga tggtctattg
      481 cgccgacgac ccgctggctg ccaaacggct ggaggaaatg ggttgcgtgg cgatcatgcc
      541 gctgggtgcc ccgatcggct ccgggctggg ggtgcagaac cccgccatgc tgaccatgat
      601 gatggaacag gtcagcgtcc cgttcctggt ggatgccggc gtgggcaccg cgtccgacgc
      661 ggcgattgcc atggaactgg gatgcgacgc cgtgctgctg aactcggcga tcgcactcgc
      721 caaggatccg atcaagatgg cacgcgcgat gaagctggcc gtggaggcgg ggcgcctgtc
      781 ctacttggcc ggacgcatgc cgcgccggat gggggccgat ccatccagcc cgctgaccgg
      841 gttgatccgc taagccggtt ccgcagtggc attcggctca ggcagcggga tcctgcgcct
      901 ggccgaggcg gtccgccgtg gttgcgaggt catagccgag gctgcgcacg aggtcgccgt
      961 gccgctgctc gaaggcgcgc acctggcccg gcgacagcac ggtccgccat ttcgagagtg
     1021 tgcgggagtg gatatgcgtg ttggccgcta gcgcggcgtc atccatgcgg gcgacgtcct
     1081 cacgcgctgc aagcgtcacc gcggcatcgc actcttccgg cccgaagccg aagtgctgga
     1141 acaccatacg catggtggcg tcgaagtttc ccgccagttc ctccaaccgg atatccagca
     1201 cggccggatc ctgccgccag gcgcgcatcg cctccaacgt gcacgcggta tagccctgca
     1261 attcgaactc cagacccgcg gcctgatcgc gatcgagcag gttttgctga taggacttgc
     1321 cgttcagccg ctccagccat ttccgcttcc accgctccgg ccggtgctgc atggagaagt
     1381 ctacgcgcgg ataggtgatc ggcggccgcg gatcgaggtc ggtattggtg caccaatgct
     1441 cctgcgtccg gcggtggtac aggtaaccgg acacccagat gtcgcgcgga tcgcggatca
     1501 gccgaatccc gcgataatcg cgcgccagcc ggaacccaag cagcgaatgc ggcaacagga
     1561 cgatatcggc ggcgggatcg atctcgtacg ccatgccata ctgctgcacc agccgcagcc
     1621 cgaactgctc ggcgacttgg cgcataaccc gatcgaacag cgtcgtgccg gatttatgat
     1681 agctgaacac cagcgcctgc ggccgtttgc gcggcgccgc gatccagcaa ttgcacgctt
     1741 gcaggacgca gatcttgccg gcgacgtcag gcggtgatga tggatgcttc agcagcggtc
     1801 ccggcgccgc gcttggcacg cgcgcggcca ggtggtgtgg cagcaacccg aagcgaagcc
     1861 cgtatggcgt gttgacggat cgcaccaggt gtcgatacgc cgtgaaattc cggccggcaa
     1921 aaggcaccgg ataaggttcg gcggcactgg tgtcccactg cacgccatgc tcgagcaaca
     1981 gccggttcaa tgcgccctga tcatcgattt cggacggcgt gcgtgcatgc accgcctcca
     2041 ggaattccac cgcccgcgga cccgcccgaa cggcaaaaac tccgcagcac agcacgaaat
     2101 tccaggcggt gaaggcctgt tccggatggt aggtgccttg cgagaacacc aggtcgagcg
     2161 agggatcggc gaagaagtca gcacgtggat cccgcagcca caccgcgtcg atgtcggagt
     2221 gaatgaagtc gatgccatgc aacgcgagcg cctggaacac gtccagccga acctgccaga
     2281 gcgctgcccg gtcccccggc tgcgggacag caacggcgac gcagccatcg cgcatcggat
     2341 gggccgtcac ggcggggtcg agcgccacca ccagcacgcg ccgcactccg gcgcggtggg
     2401 catgtgcgat ccaattttcc agcaacggga taaaggcgcc gttcgcgaac accacgggaa
     2461 ccgggccatc cggagcgagg tcgtgcgccg cggccaggag cggcgggagg aacggatact
     2521 ggagcatgac ggcggtggtg cttcttgcaa tggccgcgat ggc
//
LOCUS       DASSZH010000171         3383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_396105, whole genome shotgun sequence.
ACCESSION   DASSZH010000171 DASSZH010000000
VERSION     DASSZH010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396105"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..1723)
                     /locus_tag="VFN42_03720"
     CDS             complement(182..1723)
                     /locus_tag="VFN42_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HET8995754.1"
                     /translation="MIHQSLMGLLYGFSVALQPENLVWSFAGVLIGNLIGVLPGMGPI
                     STISILLPLTYAMHPIPSILMLAGIFYGAQYGGAIGAILLNLPCHPPHAVTCLDGYPM
                     TRQGRAGAALGVTMLCSFFAASVGIIVMILASPLLVEISFRFGPAEIFSIMLLGLLAG
                     ATMSRGSPLKGIAMVVIGLLIGVCGTDVLTGVQRFTFGLTDLDDGVELVALALGLFGV
                     AEFLRSVNRLDAVGMPMRMRMRDMRPSREELRQAIPAMLRGTAVGTLFGAIPGTGPTI
                     TTFIAYALERKVARRPERFGHGAIEGVAAPEAASHSKTQVDFIPTMSLGIPGDAVMAL
                     ILGALLIQGIQPGPQLISEHPDLFWGLIASFWIGNALLMVLNVPLIGLWVKLLRVPYR
                     FLFPSALFFIAVGVYSTQNNLFEVWEVLAFGLAGAVFNALDFQVAPILLGFVLGPLVE
                     DNFRRALLLSHGDMAVFLQRPISAGFIAASTLLIGLRLFFWLRSLRRGQAEEIAELAA
                     EVPPG"
     gene            complement(1728..2183)
                     /locus_tag="VFN42_03725"
     CDS             complement(1728..2183)
                     /locus_tag="VFN42_03725"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HET8995755.1"
                     /translation="MRSVSFKDLVGGGLMLLLGIGAAVQAAQYQIGSLRDMGPGYVPL
                     ALGILLAVTGALIMLAALRTATITPAVALPPEWRGWACICAGMVAFAVIGLYGGLLPA
                     TFACVFIAALGDRKNSLLAALVLAASMTVVCLIVFWWLLKVQLPLFRWA"
     gene            complement(2259..2975)
                     /locus_tag="VFN42_03730"
     CDS             complement(2259..2975)
                     /locus_tag="VFN42_03730"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET8995756.1"
                     /translation="MILHLLAAGASKGLVSDLQARFAAETGAELAASFMAAGAVRERF
                     QAGDPCDVVILTAALLDQLSAHGLLDGAPAPIGMVRTGIAVPAGKPVPNVETSVGLRK
                     ALLAARGIYVPDPQISTAGVHFMRVVRELGIQPQVMPYLRPFPNGATAMHNLAKATEP
                     DAIGCTQITEIKYTPGLTVAGPLPEALGLATVYAAAVAGRSVQPDAARRLVTLLTGPE
                     SLELRQAGGFEVPSGEPFGT"
     gene            complement(3013..>3383)
                     /locus_tag="VFN42_03735"
     CDS             complement(3013..>3383)
                     /locus_tag="VFN42_03735"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HET8995757.1"
                     /translation="FDAIATMAGLIKSGKVVGIATTGKTRSKVLPNLPTMSEAGVPGY
                     EAPIWLGLVAPAGTPKPIVDKLNGLISKIEEMPAVRDSWEKQGATPVIMKPAQFRDFI
                     AADIKKWAGVIKAAHIQAHG"
BASE COUNT          619 a         1175 c         1125 g          464 t
ORIGIN      
        1 gcagacacca acggagcagg cccgcgcttc ggtgcagcgg gcggaacgcg gggatgcggc
       61 cagtgccgag cggctgcggc ggcaggagca ggccgacccg gaagcggccg aggcggcgga
      121 gcaggaaatc tccgacggtc cgggccggcg cggcctgtcc cgctgacccg ccggcgcggc
      181 gctatcctgg cggcacttcg gccgccagtt ccgcgatctc ctccgcttgg ccgcggcgca
      241 ggctgcgcag ccagaagaac agccgcaagc cgatcagcag cgtgcttgcg gcgatgaagc
      301 cggcactgat cggacgttgc aggaacaccg ccatgtcgcc atgcgacagc agcaaggcgc
      361 ggcggaagtt atcctccacc agcgggccca gcacgaagcc cagcaggatc ggcgccacct
      421 ggaagtccag cgcattgaac accgcgccgg ccaagccgaa ggccagcact tcccagacct
      481 cgaacaggtt gttctgcgtg ctgtagacac ccaccgcgat gaagaacaac gcggagggaa
      541 acaggaaccg gtacggcacc cgcagcagct tcacccacag gccgatcagc ggcacgttca
      601 gcaccatcag cagcgcgttg ccgatccaga agctggcgat caggccccag aacaggtcgg
      661 gatgctcgct gatcagctgc ggaccgggct ggatgccctg aatcagcagc gcgcccagga
      721 tcagcgccat caccgcatcg cccggaatgc ccaggctcat ggtcgggatg aagtccacct
      781 gggtcttgga atgcgacgct gcctccggcg ccgccacgcc ctcgatcgcg ccgtggccga
      841 agcgctcggg ccggcgcgcc actttgcgtt ccagcgcata ggcgatgaag gtggtgatgg
      901 tcggtccggt tcccggaatg gcaccgaaca gcgtaccgac ggccgtgccg cgcagcatgg
      961 cgggaatcgc ctggcgcagt tcctcccggc tcggccgcat atcgcgcatc cgcatgcgca
     1021 tcggcatgcc caccgcatcc agccggttca cgctgcgcag gaactccgcc acgccgaaca
     1081 gacccagcgc cagcgccacc agttcgacac cgtcgtccag gtcggtcagg ccgaaggtga
     1141 agcgctgcac gccggtcagc acgtcggtgc cgcacacgcc gatcagcagg ccgatcacca
     1201 ccatggcgat ccccttcagc ggcgagccgc gcgacatggt ggcaccggcg agcaagccca
     1261 gcagcatgat ggagaagatc tccgccgggc cgaagcggaa ggagatctcc accagcagcg
     1321 gcgaggccag gatcatcacg atgatcccca ccgacgcggc gaagaaggag cacagcatgg
     1381 tcacgccgag cgccgcgccc gcccggccct ggcgggtcat cggatagccg tccaggcagg
     1441 tgacggcgtg tggcggatgg catggcaggt tcagcaggat ggcgccgatc gcgccaccgt
     1501 actgcgcgcc atagaaaatg ccggcgagca tcaggatcga cgggatcgga tgcatcgcat
     1561 aggtcagcgg cagcaggatg gagatggtgg aaatcggccc catccccggc agcacgccga
     1621 tcaggttgcc gatgaggacc ccggcgaagg accagaccag gttctccggc tgcagcgcca
     1681 cgctgaagcc gtagagcagg cccatcaatg actggtggat catcgcgtca ggcccagcgg
     1741 aacagcggaa gctgcacttt cagcagccac cagaacacga tcaggcagac gacggtcatg
     1801 ctggcggcca gaaccagagc cgccagcagc gagttcttgc gatcgcccaa ggcggcgatg
     1861 aacacgcagg cgaaggtggc cggcagcaat ccaccataca ggccgatcac cgcgaaggcg
     1921 accatgccgg cgcagatgca tgcccatccc cgccattccg gcggcagcgc caccgctggc
     1981 gtgatggttg cggtgcgcaa tgccgccagc atgatcagcg cgccggtcac cgcaagcaga
     2041 atgccaagcg cgagggggac ataaccgggt cccatgtcgc ggagggagcc gatctggtac
     2101 tgcgccgcct gcaccgcggc accgatcccc agcaacagca tcaggccgcc accgaccaga
     2161 tccttgaagc tgacgctgcg catgctgtct tcctccgttc ggctcgtgcc ggatcgtacc
     2221 acgccgccgc gcaggtaccg accctgccgc gatgcggatc aggtaccgaa cggttcgccg
     2281 gatggcactt caaacccgcc ggcctgccgc agttccaggg actcggggcc ggtcagcagc
     2341 gtcaccagcc tgcgcgcggc atccggctgc accgagcggc cggcgaccgc ggcggcatag
     2401 accgtggcca ggccgagcgc ttccggcaac ggtccggcaa ccgtcaggcc aggggtgtat
     2461 ttgatctcgg tgatctgcgt gcagccgatc gcatccggct cggtcgcctt cgccaggttg
     2521 tgcatcgccg tggcgccatt cgggaacgga cgcagatacg gcatcacctg cggctggatg
     2581 ccgagttccc gcaccacgcg catgaagtgg acgccggcgg tggagatttg cggatccggc
     2641 acatagatgc cgcgcgcagc cagcaacgcc ttgcgcaggc cgacgctggt ttccacgttc
     2701 ggcaccggct tgccggccgg cacggcgatg ccggtgcgga ccatgccgat cggcgccggc
     2761 gctccgtcca gcaggccatg cgcgctgagc tggtccagca gcgccgcggt caggatcacc
     2821 acgtcgcagg gatcgcccgc ctggaaccgc tcacgcaccg cgcccgcggc catgaaactg
     2881 gccgcgagtt cggcgcccgt ctctgccgcg aagcgggcct gcagatcgct gaccagacct
     2941 ttcgacgcac cagcggccag aaggtgcagg atcatcaccg gctcccgagg tgctgcggct
     3001 tgtcgcgccg gttcagccgt gagcctggat gtgcgcggcc ttgatcacgc cggcccactt
     3061 cttgatgtcg gcagcgatga agtcgcggaa ctgcgcgggc ttcatgatca ccggcgtggc
     3121 gccctgcttt tcccaggagt cgcgcacggc cggcatctct tcgatcttgc tgatcagccc
     3181 gttcagtttg tccacgatgg gcttcggcgt acccgccggc gccaccagac caagccagat
     3241 cggcgcttcg tagccaggca cgccagcctc cgacatggtg ggcaggttgg gcagcacctt
     3301 ggaccgtgtc ttgccggtgg tggcgatgcc gacgaccttg cccgacttga tcagccccgc
     3361 catggtggcg atggcgtcga aca
//
LOCUS       DASSZH010000172         2517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1555216, whole genome shotgun sequence.
ACCESSION   DASSZH010000172 DASSZH010000000
VERSION     DASSZH010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555216"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="VFN42_03740"
     CDS             <1..1031
                     /locus_tag="VFN42_03740"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET8995758.1"
                     /translation="ISAAAIASAERHFAHPAIRFLQGNAEQAAALLADEPAFDLIACF
                     ETVEHVADPAALLQALRRLCAPGGTIVVSCPNDPATLSPGATNPFHVRTYTFGQFREF
                     AEGILGPASDWLIETPAIGLLHYRLGDAATERPASGPTDIVRLHDAEACLQVPAQQGD
                     APSAQDCLAYLGVWGDTLPGNCAVSPMSHSGFMFPWRKLDRLELEFARVNREQQEDRR
                     QLLNFAETVTALRQSHAADQEQIGQFGQPEGQAASLAEENQPALRRYAAGRAAAEADL
                     ARTRAELAARDTELMLARQWREGVERSPFYRVLQAYVGLFRLPVVGPVLTAARRGAAQ
                     IWRGSRQT"
     gene            1200..1781
                     /locus_tag="VFN42_03745"
     CDS             1200..1781
                     /locus_tag="VFN42_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HET8995759.1"
                     /translation="MTWVDLAVLGVLAVSALLAFLRGFVREVLGIGAWIGAAAVAVWG
                     AQYARTPVERWIDSPDWAEPVALIGIFLVALIILLLVAHLLGRLVRGSPLGGLDRTLG
                     LLFGLGRGAALVVIAYILAGNVVPVDHWPNPVRDAIAMGPTYQAARWVRAQLPDGFRP
                     NVPKPPAGLRASPEALMRATPQGRALGTPRVRE"
     gene            1798..>2517
                     /locus_tag="VFN42_03750"
     CDS             1798..>2517
                     /locus_tag="VFN42_03750"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015531.1"
                     /note="Catalyzes first step of the de novo purine
                     nucleotide biosynthetic pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET8995760.1"
                     /translation="MTATAAPHDDDKLHEECGVIGLWNVPDATAVTALGLHALQHRGQ
                     EATGIVSFDGTRFHSHKGLGLVGDNFSDARVIASLPGTIAIGHNRYATTGDTVLRNVQ
                     PLYADFEFGGFAVAHNGNLTNAHVLRRALVRRGCLFQSTTDSEVFIHLIAISLYATVL
                     DRLIDALKQVMGAYSLIALTDAALIGVRDPLGVRPLIIGKLDGGGFVLASETCALDIV
                     GAEFVRDVEPGEIVVIDESGLS"
BASE COUNT          359 a          862 c          877 g          419 t
ORIGIN      
        1 acatctccgc cgcggcaatc gcctcggcgg agcggcattt cgcccatccg gcgatccgct
       61 tcctgcaagg caacgcggag caggcagcgg cgctgctggc ggatgagccc gcattcgacc
      121 tgatcgcctg cttcgaaacc gtcgagcatg tggcggatcc cgccgcattg ctgcaggcgc
      181 tgcgccggct gtgcgcgccc ggtggcacca tcgtggtaag ctgcccgaac gatccggcga
      241 cgctgtcgcc cggcgcgacc aatccattcc acgtccgcac gtacaccttc gggcagtttc
      301 gcgaattcgc cgaggggatt ctcggccccg cctccgactg gctgatcgaa actcccgcca
      361 tcggcctgct gcactaccgg ctcggcgatg ccgcgaccga gcggccggcc agcggtccca
      421 ccgacattgt caggctgcac gacgcggaag cctgcctgca ggtgccggca cagcaggggg
      481 atgccccctc ggcgcaggac tgcctggcct atctgggcgt gtggggtgac acgttgccgg
      541 gcaattgcgc tgtctccccg atgtcgcact ccggcttcat gtttccctgg cgcaaactgg
      601 accggctgga gctggaattt gcccgggtga accgggaaca gcaggaggat cgccgccaac
      661 tgctgaattt tgcggaaacc gtcaccgcgc tgcgacagtc ccacgccgcc gatcaggagc
      721 aaattgggca attcggccag ccggaggggc aggccgccag cctggccgag gagaaccagc
      781 cggcgttgcg gcgctatgca gcgggtcggg cggccgccga agccgacctg gctcggaccc
      841 gggccgaact cgccgcccgc gacaccgagc tgatgctggc ccggcaatgg cgtgaaggcg
      901 tcgagcgatc gccgttctat cgggtgctgc aggcctatgt gggcctgttc cggctgccgg
      961 tggtcggccc ggtgctgacc gcggcgcggc ggggagcggc gcagatctgg cgggggagcc
     1021 ggcagacctg accccgcgcg gcggcccgcg ccgtgccccg ccgcggcccg gcacgatcgc
     1081 gcttggcgtt tgctccataa cggcggataa gcgcttcgct tccgatggga cctgcgactc
     1141 gatgaggctt ggcatctccg gtccccgccg ctgcgccgga cgggaccact agcaagcgca
     1201 tgacctgggt ggatcttgcc gttctgggtg tgctggcggt ttccgccctg ctcgccttcc
     1261 tgcgcggttt cgtgcgggag gtcctgggta tcggcgcctg gatcggcgcc gcggcggtgg
     1321 ccgtctgggg cgcccaatac gcccgcaccc cggtggaacg ctggatcgac agtccggatt
     1381 gggccgagcc ggtggcgttg atcgggatct tcctggtcgc gctgatcatc ctgctgctgg
     1441 tggcgcatct gttgggccgg ctggtgcggg gttcgccgct tggcgggctc gaccggacgc
     1501 tgggactgct attcggtctg ggcaggggtg cggcgctggt cgttattgcc tatatcctcg
     1561 ccgggaatgt ggtgcctgtt gatcattggc cgaatccggt gcgcgacgcc atcgcaatgg
     1621 ggccaactta ccaggcggcc cgctgggtca gggcgcaact gccggatggg ttccggccga
     1681 atgttcccaa acctcctgct ggccttcggg ccagccccga agccctgatg cgtgccacgc
     1741 cgcagggccg ggccctcggc accccgcggg tccgcgaatg agaaaggcct gccaggcatg
     1801 accgccaccg ccgccccgca cgatgatgac aagctgcatg aggaatgcgg ggtcattggc
     1861 ctgtggaacg ttccggatgc caccgccgtc accgcgctgg gcctgcacgc gctgcagcac
     1921 cgcggccagg aggccaccgg catcgtcagc ttcgacggca cgcgcttcca ctcgcataag
     1981 gggctggggc tggtcggcga caatttcagc gatgcgcggg tgatcgccag cctgcccggc
     2041 accatcgcca tcggccacaa ccgctatgcc accaccggcg acacggtgct gcgcaacgtg
     2101 cagccgctgt atgccgattt cgaattcggc ggcttcgcgg tggcgcataa cggcaacctg
     2161 accaacgccc acgtgctgcg ccgcgcgctg gtgcgccgcg gctgcctgtt ccagagcacg
     2221 accgacagcg aggtgttcat ccacctgatc gcgatcagcc tgtacgccac cgtgctggac
     2281 cggctgatcg acgcgctgaa acaggtgatg ggcgcctatt cgctgatcgc gctgaccgac
     2341 gcggcgctga tcggcgtgcg cgacccgctc ggcgtgcgcc cgctcatcat cggcaagctg
     2401 gacggcggcg gcttcgtgct ggcgagcgag acctgcgcgc tcgatatcgt cggggccgaa
     2461 ttcgtccgcg acgtggaacc gggcgagatc gtcgtgatcg acgagagcgg cctgtcc
//
LOCUS       DASSZH010000173         5135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_276857, whole genome shotgun sequence.
ACCESSION   DASSZH010000173 DASSZH010000000
VERSION     DASSZH010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5135
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276857"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VFN42_03755"
     CDS             complement(<1..283)
                     /locus_tag="VFN42_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8995761.1"
                     /translation="MSQLVQPNILLVDDDDAVREITAAILEDLGYEVREASSGDAALE
                     ALERNPRVDLMMVDVSMPGMNGVDLTRVVHQKRPEVPVLLVTGYADGAAV"
     gene            527..1861
                     /locus_tag="VFN42_03760"
     CDS             527..1861
                     /locus_tag="VFN42_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HET8995762.1"
                     /translation="MTDSLTLLQDLITRARTAGADAADAVLVAGTSLQAERRLGKTEH
                     IERSEGRDLGLRVFVGQRSAVVSATSIDPSGFAGLAERAVAMAKVVPEDPYGGLAESA
                     TTLSQLPPLDLEDNAEPDSEALIARASAAEDAAMAVQGVTNSEGASAGYGRTEFVLVT
                     SAGFAGRLTRTSHSVSASALAGTGTDMQRDYDYHSTVHLADLNDPVAIGRSAGERAVA
                     RLHPRRPTTARMPVLYDPRVAGSLLGHLAGAINGAGVARGTSFLKDKLGQRIFRAGIE
                     VHDDPLRVRGLRSRPFDGEGTPTEARDLIADGVLTTWLLDSRSARQLGMRSTGHASRG
                     TGGPPSPGPSNLFLDKGELSPAELMAGIKLGLYVSELIGMGVNGLTGDYSRGCAGFMI
                     RDGVLAEPVAEITIAGNLLEMFSELTPANDLVFRRGTDSPTVRVDSMTMAGA"
     gene            1951..2877
                     /locus_tag="VFN42_03765"
     CDS             1951..2877
                     /locus_tag="VFN42_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007399213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HET8995763.1"
                     /translation="MRRTSLMLAAFAALALALAPALAHARAGSGGSFGSRGSRTWSAP
                     SATRTAPYMAAPMERSITPRGTPSYGGGYRGAPMYQRHSPFMSGLLGGLIGAGIGGLM
                     FGHGFWGGGLGFGGFIGFLIQIFLIVLAVRFLFRLVRGQGPVLANGPGMFARNGAPGY
                     GPGPLGGGSAGGPSISVGSGDYAAFEGLLQSIQAAWTAHDLNGLRAMTTPEMLSYFGE
                     QLADQESRGVRNSVTDVRLLQGDLSEAWTEGGRDYATVAMRFSMIDVTRDMAGRVVDG
                     SATEHQTATEVWTFLRASGGGHWLLSAIQQAR"
     gene            complement(2884..3603)
                     /locus_tag="VFN42_03770"
     CDS             complement(2884..3603)
                     /locus_tag="VFN42_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HET8995764.1"
                     /translation="MTEKLFLDDPRLRSAEATVQTSAPAGIVLDRTVFYARSGGQPGD
                     TGVLCWDGGETPITDTVKGDADSIVHIPSEGAALPPKGTRVTAEIDWDRRHRLMRMHT
                     AMHLLCSLIKGAAVTGGQVGADRSRLDFDLPNPPAKEWVEMELNALIAADHPVQIEWM
                     DEAVLDANPELVRTMSVQPPRGTGRLRLMRIGGGDALVDLQPCGGTHVARTGEIGRVA
                     VMKIENKGKQNRRIIIAPAAG"
     gene            complement(3831..>5135)
                     /gene="hslU"
                     /locus_tag="VFN42_03775"
     CDS             complement(3831..>5135)
                     /gene="hslU"
                     /locus_tag="VFN42_03775"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HET8995765.1"
                     /translation="SPREIVSELDRFIVGQGDAKRAVEIALRNRWRRQQLPEAMREEV
                     VPKNILMIGPTGCGKTEIARRLARLAQAPFLKVEATKFTEVGYVGRDVESIVRDLVDA
                     SLNMLREASRKEVQAKAELAAEERLITALVGEGAAADTRSKFRRMLRAGEFEQKEIEV
                     AVAEPSGSPIGQLDIPGMPPGQMINLGEMMKSMMGGRPMQKRRLTVEAARKLLEAEEA
                     DRLLDNDRLAKDAVAHAENNGIVFIDEIDKICARTSEGGFRGGDVSREGVQRDLLPLI
                     EGTTVNTKYGLVKTDHVLFIASGAFHLAKPSDLLPELQGRLPIRVELSPLSRDDLRRI
                     LTEPEHSLVKQYSALLGTETVNLVFTDDAIDALAELATDINDKVENIGARRLHTVLER
                     LLEDISFTATDRAGDTITVDSAYVNERVAPLAQKGDLSRFIL"
BASE COUNT          790 a         1739 c         1689 g          917 t
ORIGIN      
        1 ccactgcggc gccatcggca tagccagtaa ccagcagcac cgggacttcg ggccgctttt
       61 ggtgcaccac ccgggtcagg tcgacgccgt tcatcccggg catggagacg tccaccatca
      121 tcaggtcgac acgcggattg cgctccagag cctcaagcgc cgcatcgccg ctggacgcct
      181 cacgaacctc gtagcccagg tcctccagga tcgcggcggt gatctcgcga accgcgtcgt
      241 cgtcatcgac cagcaggata ttgggctgga ccaactggct catggctctt gccgcaccgc
      301 tggaccaggg atgcgggcac agcataaggg gaaccgcttc atggcgggtg gacactcgct
      361 tgccggccct ccagccgcaa ccggtcacgg gttcaagtgc gcgtgagcgg ttgggcgcca
      421 cgggcgcgcc tgcataccgc cgtttgttgc ccgccaacgg caccctgtct ggtcaggccg
      481 gcccggctgg cccacattcc gcccagatca cgcgggacct cgccggatga ccgactcact
      541 cacgctgctg caagacctga ttacccgggc acgcactgcc ggcgccgatg ccgccgatgc
      601 ggtgctggtt gccggcactt cgctgcaagc cgaacggcgg ctgggcaaga ccgagcacat
      661 cgagcgctcc gaaggccgcg atctcggcct gcgggtgttc gtcggtcagc ggtcggcagt
      721 ggtttcggcg acatcgatcg atccctccgg ctttgccgga ctggcggaac gcgcggtggc
      781 tatggcgaag gtggtacccg aggaccctta tggcggcctg gcggaaagcg ccaccacatt
      841 gagccaactg ccgccgctgg accttgagga caatgccgaa ccggattccg aggccttgat
      901 cgcgcgagcg tcggcagccg aggacgccgc aatggcggtg cagggcgtga ccaattccga
      961 gggcgcatcc gccggctatg ggcgcacgga gttcgtgttg gtgacctcgg ccgggtttgc
     1021 cggccggctg actcgcacca gccattcggt atcagccagc gccctggccg gcaccggcac
     1081 cgacatgcag cgcgattacg attaccacag cacggtccat ctggcggatc tcaacgatcc
     1141 ggtggcgatt ggccgcagcg ccggtgaacg ggcggttgcc cgcctgcacc cacgccggcc
     1201 gaccaccgcc aggatgccgg tgctgtacga tccgcgcgtg gccggcagcc tgctggggca
     1261 tctcgccggg gcgatcaacg gcgccggggt agcgcgcggg acgagtttcc tgaaggacaa
     1321 acttggccag cgcatcttca gggccggcat cgaggtgcat gacgacccgc tgcgcgtccg
     1381 cgggctgcgg tcccgtccgt tcgacggcga gggaacgccg accgaggcac gcgacctgat
     1441 cgccgatggg gtgctcacca cttggctttt ggacagccgg tcggcgcggc agttggggat
     1501 gcggtccacc gggcatgcct cgcgtggtac tggtgggcca ccctcgcccg gccccagcaa
     1561 tctgttcctg gacaagggcg aactgagtcc ggcggaattg atggccggca tcaagctcgg
     1621 cctctacgtc tcggagctga tcggcatggg cgtgaatggc ctgaccggtg actatagccg
     1681 cggctgtgcc ggattcatga tccgcgacgg cgtcctggcc gaaccggtgg cggaaattac
     1741 catcgctggc aatctgctgg agatgttttc cgaactgacg ccggccaacg acctggtgtt
     1801 ccggcgcggt accgattcgc ccacagtgcg ggtggacagc atgaccatgg ccggggcctg
     1861 agtcgcccgc cgatcgcggt cttcccccct gtgagccggt tggccgcgtg cccatatccg
     1921 tgccgcttgt tcacgagaaa gcgatcctcg atgcgtcgta cttccctgat gctggccgcc
     1981 tttgcggcgc ttgctctggc gcttgcgccg gcgctggccc acgcacgcgc cggcagcggc
     2041 ggttctttcg gcagccgcgg cagccgtacc tggtcggccc cgtcggcgac ccgcactgcg
     2101 ccctacatgg cagcaccgat ggagcgaagc attaccccgc gcggtacgcc gagctacggt
     2161 ggcggctatc ggggcgcacc gatgtaccag cggcactcgc ccttcatgtc aggattgctg
     2221 ggcgggctga tcggcgccgg cataggcggg ctgatgttcg gccacgggtt ctggggcggc
     2281 ggacttggat ttggtgggtt catcggcttc ctgatccaga tatttctcat cgtgctggcg
     2341 gtgcgcttcc tgtttcgcct ggtgcgcggg caaggcccgg tgctggccaa cgggcctggg
     2401 atgttcgcgc gcaatggtgc gcccggatat ggtccggggc cgctaggtgg cggatcggcc
     2461 ggcggtccgt caatcagcgt gggttcgggc gactatgcag cgttcgaggg cttgttgcag
     2521 tccattcagg cggcctggac cgcgcatgac ctgaacgggc tgcgtgcgat gaccactccg
     2581 gaaatgctga gctacttcgg cgagcagttg gcggaccagg agagccgcgg cgtgcggaat
     2641 tcggtgacgg acgttcgcct gttgcaggga gacctatcgg aggcctggac cgaaggcggc
     2701 cgtgattacg ccacggtggc gatgcgcttc tcgatgatcg atgttacgcg ggacatggcc
     2761 ggccgggtgg tggacgggag cgcgaccgag caccagactg cgacggaagt gtggaccttc
     2821 ctgcgcgcct ccggcggcgg ccattggctt ctgtcggcga tccagcaggc ccgctgacgc
     2881 cgattacccc gcagccggag cgatgatgat ccggcggttc tgcttgccct tgttctcgat
     2941 cttcattacc gcaacgcgac cgatttcgcc ggttcgcgcc acgtgggtcc cgccgcaggg
     3001 ctgcagatcc accaaggcgt ctccacctcc gatccgcatc aggcgaagcc ggccggtgcc
     3061 gcgtggcggc tgtaccgaca tggtacgcac cagctccgga ttggcatcca gcaccgcctc
     3121 gtccatccac tcgatctgca ccgggtgatc cgcggcgatc agcgcgttca gctccatttc
     3181 tacccattcc ttcgccggcg gattcggcag atcgaaatcc agccgcgacc ggtcggcacc
     3241 gacctgtccg ccggtcaccg cggcgccctt gatcaggctg cacagcaaat gcatcgctgt
     3301 atgcatgcgc atgagcctgt gccggcggtc ccagtcgatc tctgccgtca cgcgcgttcc
     3361 cttcggcggc agcgcagcgc cttcagacgg aatgtgaacg atgctgtcgg catcgccttt
     3421 caccgtgtcg gtgatcggcg tctcgccgcc atcccagcac agcacgccgg tatcgcccgg
     3481 ctgaccgcca gagcgggcgt agaacacggt gcgatccagc acgattccgg ccggcgcgct
     3541 ggtctgcacc gtcgcttcgg cggaacgcag gcgcgggtcg tcaagaaaga gcttttcagt
     3601 catgcgcggc ctcctcgcga ccgttctacc cgttcgggag gctggggcaa ccgcaccgtg
     3661 tcttcagcgc gccccacagc tacgcaggat cgccatcggg cagcttcgac aaaggggggg
     3721 caaccgcacc gtgtcttcag cgcgccccac aggcccggcc acccacgacc tggccgcatg
     3781 caaccaggga ggtcgtggat gaccgggctc agccggcaat gggtaaccgg ctacagaatg
     3841 aaccggctca ggtcaccctt ctgcgccagc ggcgcgacac gttcgttcac gtaggccgaa
     3901 tcaaccgtga tcgtgtcgcc ggcgcgatcc gtcgcggtaa agctgatgtc ttccagcagc
     3961 cgctccagca ccgtgtgcaa ccggcgcgcg ccgatattct ccaccttgtc gttaatgtcg
     4021 gtcgccagtt ccgccagcgc gtcgatcgcg tcatcggtga acaccaggtt caccgtttcg
     4081 gtcccgagca gggcggagta ctgcttcacc agcgagtgct ccggctcggt caggatgcgg
     4141 cgcaggtcgt cgcgcgacag cggggacagc tccacgcgaa tcggcaggcg gccctgcagt
     4201 tcgggcagca gatccgaggg ctttgccagg tggaaggcgc cggaggcgat gaacagcacg
     4261 tggtccgtct tcaccagccc gtacttggtg ttcaccgtgg tgccctcgat cagcggcagc
     4321 aggtcgcgtt gcacgccttc gcgcgatacg tcgccgccac ggaaaccacc ctccgaggtg
     4381 cgggcgcaga tcttgtcgat ctcgtcgatg aacacaatgc cgttgttctc ggcatgcgcc
     4441 accgcgtctt tcgccagccg gtcattgtcc agcagccggt cggcttcctc ggcctccagc
     4501 agcttgcgcg ccgcctcgac ggtgagccgg cgcttctgca tcggccttcc gcccatcatg
     4561 gatttcatca tctcgcccag attgatcatc tgcccgggcg gcatgcctgg tatatccaac
     4621 tgaccgatgg ggctgccgct gggttcggcg acggcgacct cgatctcctt ctgctcgaac
     4681 tctccggccc gcagcatacg gcggaacttg ctgcgggtat ccgccgcggc gccttcgccg
     4741 accagcgcgg tgatcagccg ttcttccgcg gccagctccg ccttggcctg cacttcctta
     4801 cgggatgcct cgcgcagcat gttcagactg gcatccacca ggtcgcgcac gatgctttcc
     4861 acgtcgcggc cgacatagcc gacttcggtg aacttggttg cttccacttt caggaacggc
     4921 gcctgtgcca ggcgagccag gcgacgggcg atctccgtct tgccgcagcc ggtagggccg
     4981 atcatcagga tattcttcgg caccacctct tcccgcattg cctcggggag ctgctggcgc
     5041 cgccagcgat tgcgcaaggc aatctccacc gcgcgcttgg catcgccctg gccgacaatg
     5101 aagcggtcca gctcagagac gatctcgcgc ggcga
//
LOCUS       DASSZH010000174         2096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1589268, whole genome shotgun sequence.
ACCESSION   DASSZH010000174 DASSZH010000000
VERSION     DASSZH010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589268"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFN42_03780"
     CDS             <1..207
                     /locus_tag="VFN42_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995766.1"
                     /translation="RGHAFFQRWGVGSVFLGRFFGPLRSVVPLIAGICGMPQLLFQLA
                     NVSSALVWATLILAPGVFGVRWLL"
     gene            complement(269..1459)
                     /locus_tag="VFN42_03785"
                     /pseudo
     CDS             complement(269..1459)
                     /locus_tag="VFN42_03785"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(1517..>2096)
                     /locus_tag="VFN42_03790"
     CDS             complement(1517..>2096)
                     /locus_tag="VFN42_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995767.1"
                     /translation="LFRSLLDEPFSALDAPLRARLRRELLALQQEFTATTILVTHDPL
                     EAALLADEVLVLEQGRALQAGPTDRLFRRPANEQVARLLGADNVAAGVVHDPRHITVG
                     DGVSLAVSGPPLSPGERVGWSFSPARARISPTGHYQGRVEAVAQMGVGRQITLRFGDA
                     RIRIFDGETDAPPDDPCRFEIDPHSVQVWPLL"
BASE COUNT          383 a          714 c          714 g          285 t
ORIGIN      
        1 cgcggccatg cgttcttcca gcgctggggc gtcggtagcg tgttcctcgg ccgcttcttc
       61 ggcccgctgc gctcggtggt gccgctgatc gccggcatct gcggcatgcc tcagctgctg
      121 ttccaactcg ccaatgtcag ctcggcgctg gtctgggcga cgctgatcct ggcgcccggc
      181 gtgtttgggg tgcgctggct gctgtagtgc accgagccgg cggtgggggc cggcacgatc
      241 gagagctgcg ccccttgcca gccggtaatc agagcagaaa ctccgcgcca atcagcgtca
      301 gcgccgccag cgcggcgagc ggcggcaccc aacgcggcgc ggcgaatccg cctgatgacg
      361 gcggacccag ccggtgcatc cgccagagtg ccagatccac cagcacgaac agcccgagcg
      421 tcagcagatt ggtgatggca agcaaccgct cgaacgggaa ggcgagcgcc accaaaagga
      481 cgatgctgcc ggccagccac gtcgccggtc cgggcgtgtt ggtgcgcggg ttgatccggg
      541 cgagcgtggc cggcagctgc cgcttgctcg ccatgccgta gaacaggcgg gccagcatga
      601 cgatctgcac caggacgccg ttcgccacgg caatggtgcc caacacggca aaggcggtcg
      661 cgctgcggcc tgcgaacaaa cccagcaacg gattgcgatc ggtactgccg cccaacaccg
      721 cggcggtcgc taccgcaata tacagcacca ggctgacggc gatcgcaccg aggatgccgc
      781 gcggcagcgt gcgctgaggc tgcttcacct attcggccag attggccagc gtctcgaagc
      841 caaggaaggc gaagaacgcg atgaatgccc cgctgaccgt gccggtcaac gcggtcatgt
      901 tcagcgggac cagatcaaca agctggtggt tcggtgcgtt tgccagcccc actgccgttg
      961 ccgcgacaag gccggcgatt tcgatcacac tcatcagcgc ggccagccca acgctttccc
     1021 gcacaccgag cagggcgatt ccggtgaagg tggccaccag gcccgcggcg agccagcccg
     1081 ccgaaagggg cagcagctcc tgcatgtact gcacggcgcc atgcaggatg gacgcggtgg
     1141 aaaccgtcac ggccacggtg agaaccgcac ctgcgagttg tgccaggcgc ggcgaaccga
     1201 aaccttcccg aatgaacccc acgctgccgg atgcctccgg cacgcggccc gccagttcgg
     1261 cgtaacacag cccggtcaat ccggcggcga taccggcgag caggaaggag aatggcgccg
     1321 cactgccagc gcgacggatg accgcgccgg tggcgacata aatgccggca ccgacgatga
     1381 cgcccagacc gtacagcacc agcggccaga aggtcagaac ccgccgcagc gccggtttgc
     1441 cgggtagggg gtcactcatc gagggacacg ggcatcagca caccgcttag cgccgccggt
     1501 gatgcggaag acagcgtcac agcagcggcc agacctgaac ggaatgggga tcgatctcga
     1561 accggcacgg atcatccggg ggtgcgtccg tctcgccatc gaagatcctg atccgcgcgt
     1621 cgccgaaccg caacgtgatc tgccggccga cgcccatttg cgcgaccgct tcgacgcggc
     1681 cctgataatg gccagtgggg ctgatgcgcg cgcgggcggg ggaaaaactc cagccgacgc
     1741 gctcgcccgg gctcagcggc gggccggaga cagccagcga aaccccatcg ccaaccgtga
     1801 tgtgccgcgg atcatgcacc acgccggcgg cgacgttgtc ggcgcccagc agccgcgcca
     1861 cctgctcgtt ggcggggcgg cgaaacaacc tgtcggtggg gccggcttgc agggcgcgcc
     1921 cctgttccag caccaggact tcatcggcca gtaacgcggc ttccagtgga tcgtgcgtca
     1981 ccaggatggt ggtggcggtg aattcctgct gcagcgccag cagttcccgg cgcagccgcg
     2041 cgcgcagcgg cgcatccagc gcggaaaacg gctcatcgag cagagatcgg aagagc
//
LOCUS       DASSZH010000175         2329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_464199, whole genome shotgun sequence.
ACCESSION   DASSZH010000175 DASSZH010000000
VERSION     DASSZH010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464199"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VFN42_03795"
     CDS             <1..500
                     /locus_tag="VFN42_03795"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HET8995768.1"
                     /translation="DALRILQSGDITPEQMTGSYAGAMGQTQFMPDSFLKYAVDFSGT
                     GRRDIWHDLGCVFGSTANYLAREGWQQGLPWGEIARLPANFDASLAGHDKHRTLAEWQ
                     RLGVGRTGGLPLPPATQAAVLLPGGAGGEAFLAYHPSFRAIYRYNPSDFYCISVGLIG
                     DLVMA"
     gene            497..1399
                     /locus_tag="VFN42_03800"
     CDS             497..1399
                     /locus_tag="VFN42_03800"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlpA-like double-psi beta-barrel
                     domain-containing protein"
                     /protein_id="HET8995769.1"
                     /translation="MRTAALLVGLGVALSGCVPPGTRNMASGPLHYVIGRPYQAGGVW
                     RYPRERFDGEETGLATIDTRTGGITADGEAVDPTAMAAAHPTLQLPSVVRVTNLDNGY
                     QALVRVNDRGPADPGRVIALSPRAMALLRPADPHAAHVRVDVMPSESLQLAADLGGAE
                     SPRLALVMVPLGDVVAQPLPVPPGATQAPRLRQAAAKPVPQGMVPDVAPVPLRLPETV
                     TRVAPHPGNLYVEVASFGHAEYAALLQGRLASIGAQVVLDYNAPRDASYRVRVGPLPG
                     AAAADATLARVIAAGQGDARIVVQ"
     gene            1377..>2329
                     /locus_tag="VFN42_03805"
     CDS             1377..>2329
                     /locus_tag="VFN42_03805"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HET8995770.1"
                     /translation="MPASSSNSLSRRARRSPAVLTRRVLLSSAGLAGLAIPAIAAPTK
                     QRPGGGKPAAKPAPTPPEPTSPAQTPVGPVDTAARWAVIIDFNTGATLLDKDADVRMP
                     PSSMTKLMTAYIVYAMLKAGRLKLDQMLPVSERAWRMGGSKMFVQVGSQVKVEDLIRG
                     MIVQSGNDACLVLAEAIAGSEEQFVELMNQKAKELGLTNSHFENCTGWPADNHYMSAR
                     DIATLARDIIRDFPEDYKYDSERSFTYGNAADGRAITQQNRNPLVQKGLADGLKTGHT
                     EAGGYGLCASAERNGRRVVVVLNGMSTMHQRSEEGERLLEWA"
BASE COUNT          348 a          794 c          840 g          347 t
ORIGIN      
        1 tggacgcgct gcgcatcctg cagagcggcg acatcacgcc ggagcagatg accgggtcct
       61 atgccggggc gatggggcag acgcagttca tgcccgactc cttcctgaaa tacgcggtgg
      121 atttcagcgg caccgggcgg cgcgacatct ggcacgacct cggctgcgtg ttcggctcga
      181 ccgcgaacta cctggcacgg gagggctggc agcaaggtct gccctggggc gagatcgccc
      241 ggctgccggc gaacttcgac gcctcgcttg ccggtcacga caagcaccgc acgctcgccg
      301 agtggcaacg gctcggcgtc ggccgcaccg gcgggctgcc cttgccgccg gccacgcagg
      361 ccgcggtgct gctgccgggc ggggcggggg gcgaggcgtt cctggcctat cacccgagct
      421 ttcgcgccat ctatcgctac aacccctccg atttctattg catttccgtg ggcttgatcg
      481 gcgatctggt gatggcgtga gaacggccgc cctgctggtc gggctgggcg tggcgctgtc
      541 cggatgcgtc ccgccgggga cgcggaacat ggcatccggc ccgttgcatt acgtgatcgg
      601 gcggccctat caggccggcg gcgtctggcg ctatccgcgc gagcggttcg acggcgagga
      661 gacggggctg gccaccatcg atacgcggac cggcggcatc accgcggacg gggaggcggt
      721 ggatccgacc gcgatggcgg cggcgcaccc gacgctgcaa ctgccttcgg tcgtgcgcgt
      781 gaccaatctc gacaacggct atcaggcgct ggtccgcgtg aacgaccgcg gcccggccga
      841 tcccggccgc gtgatcgcgc tgtcgccgcg ggcgatggcg ctgctgcgcc cggccgatcc
      901 gcatgccgcg catgtgcgcg tggatgtgat gccatccgag agcctgcagc ttgccgccga
      961 cctgggcggg gcggagtcgc cgcgtcttgc gctcgtcatg gtgccgctgg gtgacgtggt
     1021 ggcgcagccc ttgccggtgc cgcccggggc cacgcaggcg ccgcggctgc ggcaggccgc
     1081 ggcaaagccg gtgccgcagg gcatggtccc cgatgtggcg ccggtgccac tgcgtctgcc
     1141 cgagacggtg acgcgggtcg cgccgcaccc gggcaatctc tatgtggaag tcgcgagttt
     1201 cggccatgcc gaatacgccg cattgctgca gggacgactt gcctccatcg gggcgcaggt
     1261 cgtgctggac tacaatgcgc cgcgtgacgc ctcgtaccgg gtccgtgtcg gtcccctgcc
     1321 gggtgccgcg gcggccgatg cgacgctcgc ccgcgtgatc gcggccggtc agggcgatgc
     1381 ccgcatcgtc gtccaatagc ctgtcgcgcc gagccagaag gagcccagcc gtgttgaccc
     1441 gtcgtgtcct gctcagctca gccggccttg ccggcctcgc cattccggcg atcgcggcgc
     1501 cgaccaagca gcgcccgggg ggcggcaagc cggccgcgaa acccgcgccg acgccgccgg
     1561 aacccacctc tccggcgcag acgccggtcg gcccggtcga taccgcggcg cgctgggcgg
     1621 tcatcatcga cttcaacacc ggcgccacgc tgctcgacaa ggacgccgac gtgcgcatgc
     1681 cgccctcgtc gatgaccaag ctgatgaccg cctacatcgt ctacgcgatg ctgaaggcgg
     1741 gacggctgaa gctggatcag atgctgccgg tcagcgagcg ggcctggcgc atgggcgggt
     1801 cgaagatgtt cgtgcaggtc ggctcgcagg tgaaggtgga ggacctgatc cgcggcatga
     1861 tcgtgcagtc cggcaacgat gcctgcctcg tgctcgccga ggcgattgcc gggtcggagg
     1921 aacagttcgt cgagctgatg aaccagaagg ccaaggagct tggcctgacc aacagccatt
     1981 tcgagaactg caccggctgg cccgcggaca accattatat gtcggcgcgc gacatcgcca
     2041 ccctggcgcg cgacatcatc cgcgacttcc cggaagacta caaatacgat tcggagcgca
     2101 gcttcaccta cggcaacgcc gccgacggcc gggcgatcac gcagcagaac cgcaacccgc
     2161 tggtgcagaa gggcctcgcc gacgggctga agaccggcca caccgaggcc ggcggctatg
     2221 gcctctgtgc cagcgcggag cgcaacgggc ggcgcgtggt ggtggtgctg aacggcatga
     2281 gcaccatgca ccagcgctca gaggaggggg agcggctgct ggaatgggc
//
LOCUS       DASSZH010000176         3055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_242882, whole genome shotgun sequence.
ACCESSION   DASSZH010000176 DASSZH010000000
VERSION     DASSZH010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242882"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VFN42_03810"
     CDS             complement(<1..198)
                     /locus_tag="VFN42_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995771.1"
                     /translation="MSGTTRTLADLKDALAAQQAAQQATAPAAGTPAAPSPDAVRREP
                     KRDQWGRSYATGRRKDAVARVW"
     gene            complement(204..671)
                     /gene="rplM"
                     /locus_tag="VFN42_03815"
     CDS             complement(204..671)
                     /gene="rplM"
                     /locus_tag="VFN42_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET8995772.1"
                     /translation="MLKSTPSLKPAEVSKAWVLIDAEGVVLGRLAVLIANRLRGKHKP
                     QFTPHVDCGDNVVVINAAKVKITGNKADQSVFYYHTGYPGGIKGRTVRQRLESAHPER
                     VLEKAVERMITRGPLQRRQMKHLHIYAGDAHPHDGQQPMPLDVAALNPKNKRD"
     gene            834..2147
                     /locus_tag="VFN42_03820"
     CDS             834..2147
                     /locus_tag="VFN42_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET8995773.1"
                     /translation="MSETALRNADIDAALTEAKEAYVARNPKSFSRYVEATAVMPGGN
                     TRTVLFYEPFPIAIARAEGCRLWDMDNAEYIDFLGEYTAGIYGHSHKVIRAAVDRALD
                     GGINMGAHNLLEARFARAVCDRFGLERVRFTNSGTEANLLAITLARVFTRRRKVLVFN
                     GGYHGAVFGFAGGGSPVNAPFDYVLARYNDLDGTRALIAQHADDLAVVILEPMMGGGG
                     CIAAEPQFLAMLRQETQRVGALLILDEVMTSRLSPGGLQKVRGIQPDLTTLGKYVGGG
                     MSFGAFGGRAEIMDLFDPRRSDALPHAGTFNNNVLTMSAGLTGLTEIYTPDAAMALNQ
                     RGDTLRERLNALCRKADAPVQFTGIGSMLAVHMLRGPVRSPEDAAQGDAKLKELFFFD
                     MLAHGIWLARRGMMALCLPIGDPECDRLASAVEDFLQTRRSLLQG"
     gene            2228..2794
                     /gene="efp"
                     /locus_tag="VFN42_03825"
     CDS             2228..2794
                     /gene="efp"
                     /locus_tag="VFN42_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003617336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET8995774.1"
                     /translation="MKQQANLIRAGQVIEHEGRRWTVLKQQIITPGKGGAFIQVEMRD
                     LKSGNKTNERWRTADTVERLMTDNKDYTYSYSDGTNLVLMDPETFEEAHIPAELMGDA
                     APFLQDQMTVTVDLVEGDPVAIHLPPTVVLEVTEADPVVKGQTASSSYKPAMLANGVK
                     TQVPPFIEAGERIVVRTEDGSYVERAKA"
     gene            2810..>3055
                     /locus_tag="VFN42_03830"
     CDS             2810..>3055
                     /locus_tag="VFN42_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006116442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET8995775.1"
                     /translation="MPIRLSPHMVVMQNAAQKASKRLLRDFNEVEQLQVSVKGPSDFV
                     SQADLRAEQTLKEELNKARPGYAFLMEESGASGSENWA"
BASE COUNT          509 a          987 c         1066 g          493 t
ORIGIN      
        1 ccagacgcgg gcgaccgcgt ccttgcggcg gccggtggca taggagcgtc cccactggtc
       61 gcgcttcggc tcgcggcgga ccgcgtcagg ggagggggcg gccggggtgc cggcggccgg
      121 ggcagtggcc tgttgcgcgg cctgctgggc ggccagcgca tccttcaggt ccgccagcgt
      181 gcgggttgtt ccggacatcc gggtcagtct cgcttgttct tcgggttcag ggcggcgacg
      241 tccagcggca tcggctgctg gccgtcatgc gggtgggcgt cgccggcata gatgtgcagg
      301 tgcttcatct gccggcgctg cagcgggccg cgggtgatca tccgctccac cgccttttcc
      361 agcacccgct cgggatgtgc cgactccagc cgctggcgga cggtgcggcc cttgatgccg
      421 ccgggatagc cggtgtggta atagaatacc gactgatccg ccttgttgcc ggtgatcttc
      481 accttggcgg cattgatcac cacgacattg tcgccgcaat cgacatgcgg cgtgaactgc
      541 ggcttgtgct tgccgcgcag ccggttggcg atcaggacgg cgaggcggcc gagcacgacg
      601 ccctcggcgt cgatcagcac ccaggccttc gacacctccg ccggcttcag cgagggggtg
      661 gatttgagca ttcgggaggg agttcctgct tgagtggcgc gccttatcgg aggaatgcgt
      721 gctgcggtca aggccttggc gatgtggtaa tttgatacca cagaacccgg ggcgcggatt
      781 gcgcgccacg gccgcccgcg cgacaactgc gatacacgga cgggagagcc agcatgagcg
      841 agacggcgtt gcgcaacgcg gacatcgatg cggcgctgac cgaggcgaaa gaagcctatg
      901 tcgcgcgcaa tcccaagagc ttctcccgtt acgtcgaagc cacggcggtg atgccgggcg
      961 gcaacacgcg cacggtgctg ttctacgaac cgttcccgat cgccatcgcc cgcgccgagg
     1021 gctgccgcct gtgggacatg gacaacgcgg aatacattga tttcctgggc gaatacaccg
     1081 ctggcatcta cggccactcc cacaaggtga tccgcgccgc ggtggaccgc gcgttggacg
     1141 gcggcatcaa tatgggtgcg cacaacctgc tggaggcgcg cttcgcccgc gcggtgtgcg
     1201 accggttcgg actggagcgg gtgcgcttca ccaattccgg caccgaagca aacctgctgg
     1261 cgatcaccct ggcgcgcgtg ttcacccggc gacgcaaggt gctggtgttc aatggcggct
     1321 atcatggcgc cgtgtttggc ttcgccggcg gcggcagccc ggtgaacgcg ccattcgact
     1381 atgtgctggc gcgctacaac gacctggacg gcacgcgcgc gctgatcgca cagcatgccg
     1441 atgatctggc ggtggtgatc ctggagccga tgatgggcgg cggcggctgc atcgcggccg
     1501 aaccgcagtt cctggcgatg ctgcggcagg agacccagag ggtgggcgca ctgctgatcc
     1561 tggacgaggt gatgacgtcc cgcctgtcgc cgggcggcct gcagaaggtg cgcggcattc
     1621 agccggacct gaccacgctg ggcaaatatg tcggcggcgg catgtcgttc ggcgcattcg
     1681 gcggccgggc cgaaatcatg gatctgttcg acccgcgccg gtccgacgcc ctgccgcatg
     1741 cgggaacctt caacaacaat gtgctgacca tgtcggccgg actgaccgga ctgaccgaga
     1801 tttacacccc ggacgccgcg atggcgctga accagcgcgg cgatacgttg cgcgagcggc
     1861 tgaacgcgct gtgccgcaag gctgacgcgc cagtccagtt caccggcatc ggctcgatgc
     1921 tggcggtgca catgctgcgc ggcccggtgc gcagcccgga ggacgcggcg cagggggatg
     1981 ccaagctcaa ggagctgttc ttctttgaca tgctggcgca cggcatctgg ctggcgcggc
     2041 gcggcatgat ggcgctgtgc ctgccgatcg gcgacccgga gtgcgaccgg ctggccagcg
     2101 cggtggagga tttcctgcaa acccgccggt ccctgctgca gggctgaccg ccccctgcct
     2161 tgcccctccg ccgctcgcgt tctagaagcc ggcacccata ttccccgacg cagcagcaga
     2221 gtgcccgatg aagcagcagg ccaacctgat ccgcgccggc caggtgatcg agcatgaagg
     2281 ccgacgctgg accgtgctga agcagcagat catcaccccc ggaaaaggcg gcgccttcat
     2341 ccaggtggag atgcgcgacc tgaaatccgg caacaagacc aacgagcgct ggcgcaccgc
     2401 cgacacggtc gagcggctga tgacggacaa caaggattat acctactcct actccgacgg
     2461 caccaacctg gtgctgatgg acccggagac cttcgaggaa gcccatatcc cggccgagct
     2521 gatgggcgac gccgcgccct tcctgcagga ccagatgacg gtgaccgtgg acctggtgga
     2581 gggtgacccg gtggcgatcc acctgccgcc gaccgtggtg ctggaggtga ctgaggccga
     2641 cccggtggtg aagggccaga ccgcgtccag ctcctacaag ccggccatgc tggccaatgg
     2701 cgtgaagacc caggtgccgc ccttcatcga ggctggggag aggatcgtgg ttcgcacgga
     2761 agacggcagc tacgtggagc gcgcgaaggc ctgaccggcc gcgcacccca tgccgattcg
     2821 cctgtccccc cacatggtcg tgatgcagaa cgccgcccag aaggcgtcga agcggctgct
     2881 gcgcgacttc aacgaggtgg agcagctaca ggtcagcgtc aaaggcccct ccgatttcgt
     2941 ttcccaggcc gacctgcgag ccgagcagac cctgaaggag gagctgaaca aggcgcggcc
     3001 cggttatgcg ttcctgatgg aggaatccgg cgcctccggc tcggagaatt gggcc
//
LOCUS       DASSZH010000177         2230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_276951, whole genome shotgun sequence.
ACCESSION   DASSZH010000177 DASSZH010000000
VERSION     DASSZH010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276951"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="VFN42_03835"
     CDS             complement(<1..794)
                     /locus_tag="VFN42_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HET8995776.1"
                     /translation="MAASKKRQAAEAFRGEGVSMADAVIVSAVRTPVGRAPKGALSTT
                     RPDDLAAIVINAALERVPQLDKREIEDVILGCAQPEGEAGFNIARFAALRAGLPIEVP
                     GVTVNRLCSSGLEAIALADMRIRAGGEGVVIAGGAESMSLIPMGGAKPSPNPWLAEHY
                     PASLLTMGLTAERVARHYNVSREDQDAFALASHRKALAAQAAGKFDGDLAPVSITTAT
                     PTARAGKPQVTETMFAADEGPRADTSAEALARLKPVFHAQGTVTAG"
     gene            complement(816..1421)
                     /locus_tag="VFN42_03840"
     CDS             complement(816..1421)
                     /locus_tag="VFN42_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995777.1"
                     /translation="MMLPRHRAGLVLLIVLVVSAAAATQPDSGLVTRGAVEIGGKQVP
                     YVVRHLPVASFPELPAAVADELTRRGCLVPQTYEAHQPENVVHGSFQQPGSSDWAVLC
                     SVDGEVKLLVFLGSDAAAPMTIGSAAETQRVQPYPGGSAMGFNWGIDRAGAEAVREAQ
                     TGLRPRPARIDHDALADSVVEQRTIYHYYKSGHWVLLDMPD"
     gene            complement(1472..>2230)
                     /locus_tag="VFN42_03845"
     CDS             complement(1472..>2230)
                     /locus_tag="VFN42_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HET8995778.1"
                     /translation="AIRGFQQMTQAIKFCPRPVVVAPFGMTFGGGAEMCLHAARRQPH
                     AETYIGLVEAGVGLIPGGGGTKEMLLRAVDAAVAVVPPDPKDPPSRFAQSAEMLAALR
                     RTLETIAMAKVSTSAVEARALGLFSPADRITMNRERLLLDAKAQALALVEAGYAAPVP
                     RTQIPAPGLAALATLETGIFMMGEGGFASEHDRKVAKWAAYILAGGRVTAGSLVSEQY
                     LLDLEREAFLSLCGERKTLERIAFTLKTGKPLRN"
BASE COUNT          374 a          844 c          649 g          363 t
ORIGIN      
        1 tttcccgccg ttaccgtgcc ctgcgcatga aaaaccggct tcaatctcgc cagcgcttcg
       61 gcggaggtgt cggcgcgcgg accttcgtcc gctgcaaaca tagtctccgt cacctgcggc
      121 ttgccggctc gggcggtggg cgtcgccgtg gttatggaaa ccggtgccag atccccatcg
      181 aacttgccgg ccgcctgcgc cgcaagcgcc ttcctgtggc tggccagggc gaaggcgtcc
      241 tgatcttcgc ggctcacgtt gtagtgccgg gcaacgcgct ccgctgtgag ccccatggtg
      301 agcaaagacg cgggatagtg ctccgccagc cacgggttgg ggctcggctt ggcgccgccc
      361 atcggtatca ggctcatcga ctccgcgccc ccggcaatca ccacgccttc gccgcccgcg
      421 cgaatgcgca tgtcggccag cgcaatcgcc tccagccccg acgagcacag acgattcacg
      481 gtcaccccag gcacctcaat gggcagcccc gcgcgcagcg ccgcgaaccg cgcgatgttg
      541 aaaccggcct cgccctccgg ctgcgcgcat cccagaatca cgtcctcgat ctcgcgcttg
      601 tcgagctgcg gcacgcgctc cagcgccgcg ttgatgacga tggccgccag atcgtccggc
      661 cgcgtggttg acagcgcacc cttgggcgcg cgccccaccg gcgtccgcac cgcgctcaca
      721 attaccgcat ccgccatcga tactccttcg ccccggaaag cctctgccgc ttgtctcttc
      781 ttagacgccg ccacgccggc tggtcaacgc caaggttaat ccggcatgtc cagcagcacc
      841 cagtggccac tcttgtagta gtgataaatt gtgcgctgct ccacgaccga atccgccagc
      901 gcatcgtggt cgatccgcgc aggccgcggc ctcaggccgg tctgcgcctc ccgcaccgcc
      961 tccgcgccgg cgcgatcgat gccccagttg aaacccattg ccgaaccgcc gggatagggc
     1021 tgcacacgct gggtctccgc cgccgagccg attgtcatcg gcgcggccgc atcgctgccc
     1081 aaaaagacca gcagctttac ctcgccgtcc accgagcaca gcaccgccca gtccgacgag
     1141 ccgggttgct ggaagctgcc atgcactacg ttctccggct ggtgcgcctc ataggtctgc
     1201 ggaaccaggc aaccgcggcg cgtcagctcg tccgcaacag cggccggcag ctcaggaaac
     1261 gaagccaccg gcagatgccg cacgacgtag ggaacctgct taccgccaat ctccacagct
     1321 ccgcgtgtta ccaggccgga atcgggttgg gttgcggctg ccgcagacac gacgagcaca
     1381 atcagcaaca ccagacccgc tctgtgcctc ggcagcatca accctcgttc tctgctcacc
     1441 cacccgcgaa ctacgaatta tgcaccgcgt actagttccg cagcggcttg cctgtcttca
     1501 gcgtgaaggc gatccgctcc agcgtcttgc gctctccgca gagactcagg aacgcctctc
     1561 gctccaggtc gagcagatac tgttcgctca ccagcgatcc cgctgtcacg cggccgcccg
     1621 ccagaatgta cgcagcccac ttcgccacct tcctatcgtg ctcgctggcg aatccacctt
     1681 cgcccatcat gaagatgccg gtttcaagcg tagccagcgc ggccaggccg ggcgcgggaa
     1741 tctgcgtgcg cggcaccggc gcggcatagc ccgcctccac cagcgccagc gcctgcgcct
     1801 tggcgtccag cagtagccgc tcgcggttca tggtgatgcg atccgccggc gagaacagcc
     1861 ccagcgcgcg cgcttctacc gcggaagtgg acacctttgc catggcaatg gtctccagcg
     1921 tacgccgcaa cgccgccagc atctccgcgc tctgcgcaaa gcgcgacggt ggatctttgg
     1981 gatcgggcgg gaccaccgcc actgcagcgt ccaccgcgcg caacagcatc tccttggtgc
     2041 cgccaccccc agggatcagg ccgacgccgg cttccacaag tccgatataa gtctccgcat
     2101 gcggctgccg ccgcgccgcg tgcagacaca tctccgctcc cccgccgaag gtcatcccga
     2161 acggcgcaac cacaaccggc cgcgggcaga acttgatggc ctgcgtcatc tgctggaagc
     2221 cgcgaatggc
//
LOCUS       DASSZH010000178         2253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_771218, whole genome shotgun sequence.
ACCESSION   DASSZH010000178 DASSZH010000000
VERSION     DASSZH010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771218"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..976
                     /locus_tag="VFN42_03850"
     CDS             77..976
                     /locus_tag="VFN42_03850"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HET8995779.1"
                     /translation="MKVLWQSRPDRQSHGFRVTSHPVAEAAARTFGRGRHAGMLRLLD
                     AVLPHCRRMVEFGAYIGLNALYAATWCREVFAFEPSPTNHELLACNVAANPELAPRIR
                     LFRHGIGQDDGYVPLYAKGLADAGTSVFREVERADVVCARQHAIVPLRDAGAVLRELG
                     LDDRTLLHIDVAGAEYTIVPQIAELLAERKPWLLVAFHPFNLVAGRDPYRTALLRLRC
                     GMEAAEALAAYRFIHLYEADTGWRTVGPAERMDFLRQYLLSAKPVPRIASSQYGFVHT
                     LAFSDQPLPADEREGQAAPQPTR"
     gene            complement(961..2058)
                     /locus_tag="VFN42_03855"
     CDS             complement(961..2058)
                     /locus_tag="VFN42_03855"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HET8995780.1"
                     /translation="MKIIKVRTDILRIPEDDPLADMPEDAARLRPVVILRMQTDAGVE
                     GIGLTFYGGAMTGSLRVAVDELGALTIGEDPLRIEAIVRKLRLAAGDSCGPAGIFLLA
                     LAAIDTALWDIKGKALNQPLWKLLGGTRDRVATYASGSLRRGLTDDQAAKAAHTLVQK
                     GFVEMKTQMALPGNPSPADEVRRVRVVREAIGPDIKLMCDINQRWRVEQAIDIGKRVE
                     DVGLFWLEDVTTCDDYQGLARINEALVTPIAGGEYLWGIAPFRQMIEARSVDYIMIDL
                     CRVGGPTQWMKVAGMAEAFNLPVVSHVMPEMHLHLVAAVPNGLTVEYMPWMLKLYQET
                     PAIEQGQLVLPDKPGLGLMFDEKAVAAYRVG"
BASE COUNT          355 a          798 c          717 g          383 t
ORIGIN      
        1 gaccgtcagg aatatcttaa cagctgtcaa tgtaatctct cttcatcggc ggcagatgga
       61 ggtgggcgtg gcgggcatga aggttttgtg gcagtcacgt ccggatcggc aaagtcatgg
      121 cttccgggta acatcgcatc ccgtcgccga agccgccgcc cggacgttcg gccgcggcag
      181 gcacgccggc atgctccggc tgctcgatgc ggttctgccg cactgccgcc gcatggtgga
      241 attcggcgcc tatatcggcc tcaatgcgct gtatgccgcc acctggtgcc gcgaagtctt
      301 tgctttcgaa cccagcccca ccaatcatga gctgctggcc tgcaatgtcg ccgccaaccc
      361 cgagctggct ccccgcatcc gactgttccg ccatggcata gggcaggacg acggctacgt
      421 gccgctctat gcgaaaggcc tggcggatgc cgggacctcg gtgttccgcg aggtggagcg
      481 cgcggacgtg gtctgcgcgc ggcagcacgc catcgtgccc ctgcgggatg ccggggccgt
      541 cctgcgcgaa ctcggcctgg acgaccggac cctgctgcat atcgacgtgg cgggcgcgga
      601 atacaccatc gtgccgcaga tcgccgagtt gctggcggag cggaagccct ggctcctcgt
      661 cgcgttccac ccgttcaacc tggtggccgg ccgcgatccc tatcgcaccg cactgctgcg
      721 gctgcgctgt ggcatggagg ccgccgaggc gctggccgcg taccgcttca tccatctcta
      781 tgaggcggat acagggtggc gcacggtcgg acccgccgag cgcatggatt tcctgcgcca
      841 gtacctgctc agcgccaagc ccgtgccgcg gatcgcgtca tcccagtacg ggtttgtgca
      901 cacactggcc ttctccgatc agccgctgcc ggcggacgag cgtgaaggtc aggcggcgcc
      961 tcagcccacg cgataggccg ccaccgcctt ctcgtcgaac atcagcccca ggccgggctt
     1021 gtccggcagc acaagttgac cctgctcgat cgccggggtc tcctggtaca gcttcagcat
     1081 ccagggcatg tactccacgg tcagcccgtt cggcaccgcc gcgaccagat gcaggtgcat
     1141 ctccggcatc acgtgactga cgaccggcag gttgaacgcc tccgccatgc cggcgacctt
     1201 catccactgc gtcggtccgc ccacccggca caggtcgatc atgatgtaat cgaccgaccg
     1261 cgcctcgatc atctggcgga acggcgcgat gccccacagg tactcgccac cggcgatcgg
     1321 ggtgaccaac gcctcgttga tgcgcgccag gccctggtag tcgtcgcagg tggtcacgtc
     1381 ctccagccag aacaggccga catcctccac ccgcttgccg atatcgatcg cctgctccac
     1441 ccgccagcgc tggttgatgt cgcacatcag cttgatgtcc ggcccgatcg cctcgcgcac
     1501 cacccgcacc cggcggacct cgtcggcggg tgacggattg ccgggtagcg ccatctgggt
     1561 cttcatctcg acgaagccct tctgcaccag cgtgtgtgcc gccttggccg cctgatcgtc
     1621 ggttagcccg cggcgcagcg aacccgaagc gtaggtggca acccggtcgc gcgtgccgcc
     1681 gagcagcttc cagaggggct ggttcagcgc cttgccctta atgtcccata gcgcggtatc
     1741 gatcgccgcc aacgccagca ggaagatgcc ggccggaccg caagaatctc cggcggcgag
     1801 gcgcagcttg cgcacgatcg cctcgatgcg cagcggatcc tcgccgatgg tgagcgcgcc
     1861 cagctcgtcc accgcgacgc gcaggctgcc ggtcatggcg ccgccgtaga aggtgagccc
     1921 gatgccctcg acgccggcgt ccgtctgcat gcgcaggatc acgacggggc gcaggcgcgc
     1981 ggcgtcctcc ggcatgtcgg ccagcggatc gtcttccggg atgcgcagga tatcggtgcg
     2041 aaccttgatg atcttcatac tgggtctcct ccccagcgcc agcatagccg caggaagggc
     2101 ggcggcaatc cgaccgcctc aggtgcgcga cagcccttcc gcgatccgct ccgccacggc
     2161 cgcgatggtc atgcatgggt gaaagccgat cgaggtgggg atcacggacc catccgccac
     2221 gaacaatccc ggatggccat gtgcctcccc gtg
//
LOCUS       DASSZH010000179         3097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1487010, whole genome shotgun sequence.
ACCESSION   DASSZH010000179 DASSZH010000000
VERSION     DASSZH010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487010"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="VFN42_03860"
     CDS             <1..1243
                     /locus_tag="VFN42_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET8995781.1"
                     /translation="IRPWLESMRFGVTIHPNPQPGFGPILTAERLEDPDRPTVLTYGH
                     GDTVRGLEDQWSAGLAPWTLTEQGDRWYGRGTADNKGQHALNLTALEAVLAERGGKLG
                     CNLKLVLETCEERGSIGLRDFVAAHRDLLAADVLIGSDGPRVAPDTPTIATGTRGTFH
                     FDLVVALRPGGVHSGHWGGLTTDPALVLAHALGCIADRKGRILVRDWLPPNGLPAAVR
                     AVLEGCPVAGGGDAASIDPAWGEPGLSSAEKIYGWASFIVLAMLSGRPENPVNAVAPD
                     ARAHCQIRYTVDSDPAGFEPALRRHLDAHGFQSVRIERAGVRMPASRTDPAHPWVRYA
                     VDSMQRTLGRRVQVIPNSSGGLPGDVFIDHLDVPLVWVPHSYNGCKQHGPDEHLLTAP
                     AREGIAAIAGLWWDLGEPGTP"
     gene            1320..1805
                     /locus_tag="VFN42_03865"
     CDS             1320..1805
                     /locus_tag="VFN42_03865"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HET8995782.1"
                     /translation="MQAFHAVFGSQHHVLWWQECLRALLMFAYGLVLMRSSGRRTFGR
                     WSALDIVVSIILGSSLSRALTGSAPLGGTMAAMAVMIGLHWVLARAAAQSRKLAGTLE
                     GEPIALVQAGRRNEPAMRHFSVSAPDLEEALHGAGIMDIAEARLALLEPSGKITVLKQ
                     G"
     gene            complement(1983..>3097)
                     /locus_tag="VFN42_03870"
     CDS             complement(1983..>3097)
                     /locus_tag="VFN42_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET8995783.1"
                     /translation="PFDADAMLEGLRVWVECESPTYDPASVNRMMDIAARDLIVAGAR
                     IERVAGRMGFGDCVRASFPHTRQNEPGILVMGHLDTVHPVGTLRDLPFRREGNRAWGP
                     GICDMKGGNYLALEAIRQLARAGIATRLPVTMLLTSDEEVGSPSTRDLIEAEAARHKY
                     VLVPEPARADGGVVTGRYAIARFNLESTGRPSHAGARLAEGRSAIREMARKLIEIEDM
                     TTEDCTFSVGVIHGGQWVNCVTTTCTAQALSMAKRQEDLDRGVAAMLALRASGNDVQF
                     QVTRGVTRPVWEPNAKVMALYEMARGIARGLGYDITSQSSGGGSDGNFTGAMGIPTLD
                     GLGVAGAGPHTLNEHILVDSLEYRGRLMAGLLATLA"
BASE COUNT          480 a         1035 c         1070 g          512 t
ORIGIN      
        1 gattcgaccg tggctggaga gcatgcgctt cggcgttacg atccatccca atccgcaacc
       61 ggggttcggc cccatcctca cggccgagcg cctcgaggat ccggatcgtc cgacggtcct
      121 cacctacggg cacggcgaca cggtgcgcgg cctggaagac caatggagcg ccggcctcgc
      181 cccctggacg ctgaccgagc agggcgaccg ctggtacggc cgcggcacag ccgataacaa
      241 gggccagcac gcgctgaacc tcaccgcgct ggaagccgtg ctggcggagc gcggcggcaa
      301 gcttggctgc aacctgaagc tggtgctgga gacctgcgag gaacgcggct ccatcggcct
      361 gcgcgatttc gtcgccgcgc atcgcgacct gctggcggcc gacgtgctga tcggcagcga
      421 cggcccgcgc gtcgcgccgg acacccccac catcgcgacc ggcacgcgcg gcaccttcca
      481 tttcgacctg gtcgtcgccc tgcgtcccgg cggggtgcat tccggccact ggggcgggct
      541 gacgaccgac ccggcgctgg tgctggcgca tgcgctgggc tgcatcgccg accgcaaggg
      601 acgcatcctg gtgcgcgact ggctgccgcc gaacggcctg cccgccgcgg tccgcgcggt
      661 gctggaaggc tgcccggtgg cgggcggcgg cgatgccgcc agcatcgatc cggcctgggg
      721 cgagccaggc ctgagttcgg cggagaagat ctacggctgg gccagcttca tcgtgctggc
      781 gatgctgtcc ggccggccgg aaaacccggt gaatgccgtg gcaccggacg cgcgggcgca
      841 ctgccagatc cgctacacgg tggacagcga tccggccggc ttcgagcccg cgctgcgccg
      901 ccacctggac gcgcacggtt tccaatccgt gcgcatcgag cgcgccgggg tccgcatgcc
      961 ggccagccgt accgatccgg cgcatccctg ggtgcgttat gccgttgaca gcatgcagcg
     1021 cacgctgggc cgtcgcgtcc aggtgatccc caattcatcc ggcggattgc cgggcgatgt
     1081 gttcatcgat cacctggatg tgccgctggt gtgggttccg cacagctaca acggctgtaa
     1141 acaacacggt ccggacgaac acctgctgac ggcgccggcg cgcgagggca tcgcggccat
     1201 tgccgggctg tggtgggacc tgggggagcc cggtacgccc tgacgcgcta tggccgaggg
     1261 cttgcggagc gaggcgtcac aatcgcaacg caagcccaag gcggaggcgc aatagagcca
     1321 tgcaagcgtt ccatgcggtg tttggcagcc agcaccatgt gctctggtgg caggaatgtc
     1381 tgcgtgccct gctcatgttt gcttatgggc tggtgctgat gcggagttcc gggcggcgca
     1441 ccttcggacg atggtcggca ctggatatcg tggtttcgat cattctcggg tcgagcctca
     1501 gcagagcgct gaccggcagt gcgccgttgg gtggcaccat ggcggcgatg gcggtgatga
     1561 tcgggctgca ctgggttctg gcacgcgccg ccgcccaatc acggaaattg gctggcacct
     1621 tggaaggcga accgatcgcc ctggtgcagg cgggacgccg caatgaacca gcaatgcgcc
     1681 atttctccgt ctccgctccc gacctggagg aggcactgca tggcgctggc atcatggaca
     1741 tagccgaagc gcgattggcc ttgctggagc caagcggcaa gatcacggtg ctgaagcaag
     1801 gatgatcacc gtgcttgatg gcctccgtca tgccggcgca tgcggacatc cgcggattgc
     1861 ttcgccgcat gaggcggcgt gcaaccgtga gcttacgtaa caagtcgtgg gggaactggc
     1921 atttaccgat gcgcgctggc atgggctggc ccgtggcccg gcgtggtgac ttgcgaacct
     1981 cgttatgcca gcgtcgccag caatccggcc atcagcctgc cgcggtattc caggctgtcc
     2041 accaggatgt gctcgttcag cgtatgcggg cccgcgccgg caacgccgag cccgtccagg
     2101 gtcgggatgc ccatcgcacc ggtgaagttg ccgtccgatc cgccgccgga actctggctg
     2161 gtgatgtcgt agcccagccc gcgcgcaatg ccgcgcgcca tttcatacag cgccatcacc
     2221 ttggcattcg gctcccacac cgggcgtgtc acgccacggg tcacctggaa ctgcacgtca
     2281 ttgccggaag cgcgcagcgc cagcatcgcc gccacgccgc gatccaggtc ctcctgccgc
     2341 tttgccatgc tcagcgcctg cgcagtgcag gtggtggtga cgcaattcac ccattgtccg
     2401 ccatggatca caccgacgct gaaggtacaa tcctccgtcg tcatgtcttc gatctcgatc
     2461 agcttgcggg ccatttcgcg gatggcggag cgtccctcgg caagccgtgc accggcgtgg
     2521 ctggggcgac cggtggattc caggttgaag cgcgcgatgg cgtagcggcc ggtgacgacg
     2581 ccgccatcgg cgcgcgcggg ttccgggacc agcacgtatt tgtgccgcgc ggcctcggct
     2641 tcgatcaggt cgcgggtgga tgggctgccg acctcctcat cgctggtgag cagcatggtc
     2701 accggcaagc gcgtggcgat tccggcccgc gcaagctggc ggatcgcctc cagcgccaga
     2761 tagttgccgc ccttcatgtc gcagatgccg ggtccccagg cacggttgcc ctcgcggcgg
     2821 aacggcaggt cgcgcagcgt gccgacggga tgcaccgtat cgagatggcc catcaccagg
     2881 atgccgggct cgttctggcg cgtatgcgga aaactggcgc gcacgcaatc accgaaaccc
     2941 atgcgtccgg cgacccgctc gatgcgcgcg ccggcgacga tgaggtcgcg cgccgcgatg
     3001 tccatcatgc ggttgacgga ggcggggtcg taagtggggc tttcacattc cacccagacg
     3061 cgcaggcctt ccagcatggc gtcggcgtcg aacggga
//
LOCUS       DASSZH010000180         2202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_907359, whole genome shotgun sequence.
ACCESSION   DASSZH010000180 DASSZH010000000
VERSION     DASSZH010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907359"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..1744
                     /locus_tag="VFN42_03875"
     CDS             254..1744
                     /locus_tag="VFN42_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HET8995784.1"
                     /translation="MTMMLTRRGLFASASLAAASAVTPRGARALSASPIRLVAERRSL
                     DINGKAASVFGLRQPDGTPGVLLAPDQRFLVALANRTDEPTIIHWHGQTPPVRQDGVA
                     VTGLESLITPGATQGYDFAPRPGTHWMHSHQGLQEQLLMAAPLVVRTAEDMRADAQEV
                     VVLLHDFTFRNPHEVLAKLTTGADTAEAKGGGQDHAMSGMDMGSHAAPNPPATSGDAG
                     TARTGMVMDLNDIDFDAYLANDRTLADPLVVWTERSGRVRLRLINGAASTAFWLDLGS
                     LDGIVVAVDGDPVRPVPVRRFPMAQAQRVDVVVQMPAAGGAFPVLAQREGDRQRTGII
                     LATPGARIASLPARADTKVGAVDLSLERQLRAAQPLPPRRADIVHPVALTGSMMPYRW
                     GLNGRTWADHTPLAVSQGQRVELDIVNRSRMAHPMHLHGHHFQVVALNGQALSGAMRD
                     TVLVPVQGRVRVAFDADNPGRWLFHCHNLYHMATGMMTEVAYTGFA"
BASE COUNT          337 a          810 c          723 g          332 t
ORIGIN      
        1 gtcgcggctt tcatggtcaa gctcccctcc tccactgcgc ccggacaatc tgaccggccg
       61 ttaggacatt tgcgctgacc tgtgccttga tccacatcat gctttcccgg tcggccggat
      121 aaggaggtcg gagcaggatg cgcgcgccgg acgtgaccgg gaccgggctg ccgatcacac
      181 cttcgatccc gccaccttgg gggcccgtct gaaagccaca tcacgcgcac cttcctgacg
      241 aacggaaccg accatgacca tgatgctcac ccgacgcggc ctgttcgcgt ccgcctcgct
      301 cgccgccgcc agcgccgtta cgccgcgcgg cgcccgcgcc ctgtcggcgt cacccatccg
      361 gctcgttgcc gagcgccgca gcctcgatat caatggcaag gcggcctccg tgttcgggct
      421 gcgccagccc gacggcacgc cgggcgtgct gctggcgccg gaccagcgct tcctcgtcgc
      481 gctggccaac cggaccgatg agcccaccat catccactgg cacgggcaga cgccgcctgt
      541 gcggcaggac ggcgtcgctg tcaccggcct tgaatccctg atcacgcccg gtgccactca
      601 aggctacgac ttcgcgcccc gcccaggcac gcactggatg cattcgcatc agggcttgca
      661 ggagcagctt ctgatggcgg caccgctcgt cgtgcgaacg gcggaggaca tgcgcgccga
      721 cgcgcaggaa gtcgtggtgc tgctgcacga cttcaccttc cgcaatccgc acgaggtgct
      781 cgcgaaactg accacgggcg ccgacacggc ggaggcgaaa ggcggcgggc aggaccacgc
      841 catgtccggc atggacatgg gcagccatgc cgcgcctaat ccgcccgcca catccggcga
      901 cgccggcacc gccaggaccg gcatggtcat ggacctgaac gacatcgact tcgacgccta
      961 tctcgccaac gaccgcacgc tcgccgaccc gctggtggta tggacggagc ggagcggtcg
     1021 cgtccgcctg cggctgatca acggtgccgc ctccaccgca ttctggctcg accttggcag
     1081 cctggacggt atcgtcgtcg cggtggacgg cgatccggtg cggccggtcc ccgtccgccg
     1141 cttcccgatg gcgcaggcgc agcgggtcga tgtcgtcgtg cagatgccgg ccgccggcgg
     1201 cgccttcccg gtgctggccc agcgcgaggg cgaccggcag cgcaccggca tcatcctcgc
     1261 cacacccggt gcgcgcatcg ccagtctgcc agcacgcgcg gataccaaag tcggcgcggt
     1321 ggatctgtcg ctggagcggc aactccgcgc agcccagccg ctcccgccgc ggcgcgccga
     1381 catcgtgcat cccgtggcgc tgaccgggtc gatgatgccg tatcgctggg gcctcaacgg
     1441 ccgcacctgg gcggaccaca cgccgctggc cgtctcgcag ggccagcgcg tcgaactcga
     1501 catcgtgaac cgcagccgga tggcgcatcc gatgcacctg cacggccacc atttccaggt
     1561 cgtcgcgctg aacggacagg cgctgtccgg cgcgatgcgc gacaccgtgc tggtgccggt
     1621 ccaggggcgc gtgcgggtcg ccttcgacgc cgacaacccc ggacggtggc tgttccactg
     1681 ccacaacctc taccacatgg cgaccggcat gatgaccgag gtcgcctata ccggcttcgc
     1741 ctgagacgac cgacggccgc gcccgcagga cgcgaccgcg ctccccggcc gggttgatgg
     1801 catcgcttga tctggctcaa tgaaccttgg cgcgttccgg ataacgtgtg gccacgcggg
     1861 gtgtccgagc gcagaccggg tgccggacgg cgtcatcacg actgccgcgg ccacccgcgt
     1921 ggaagagtgc ctgccgcgct ggctcaatcg cggccgctgt gccgtgaatc gaatccccgc
     1981 caaaggtctt gtcggtcccg tgccccaggg cgagcgggca aaatgatccc gtccgggtct
     2041 gcgccccgcc gttgcaagga aagacagcga aaggagtttc agatgctctc gacacctcgt
     2101 gttgctctcg ccgcgatgat cctggccgcc ccgttcggcg tcgcgaacgc ggcgacacag
     2161 gcgcaggatc aggctggaca gtccgccggg gcgactctgg gg
//
LOCUS       DASSZH010000181         2918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_396337, whole genome shotgun sequence.
ACCESSION   DASSZH010000181 DASSZH010000000
VERSION     DASSZH010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396337"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /gene="urtB"
                     /locus_tag="VFN42_03880"
     CDS             <1..613
                     /gene="urtB"
                     /locus_tag="VFN42_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010668210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HET8995785.1"
                     /translation="RPLETLLATWGLSLVLQQAVRSIFGPTNRDVDTPWWMSGDTHLG
                     GLTLTWNRMYIMLFAALVLAALMAALRYTPLGLRMRAVTQNRRMAAAMGIRTPWIDAL
                     TFGLGSGVAGIAGVALSQIDNVSPNLGQNYIIDSFMVVVFGGVGNLWGTVFAAMTLGI
                     VNKFLEPFAGAVLGKIVVLVLLILFIQRKPRGMFPLKGRAVEA"
     gene            750..1895
                     /gene="urtC"
                     /locus_tag="VFN42_03885"
     CDS             750..1895
                     /gene="urtC"
                     /locus_tag="VFN42_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HET8995786.1"
                     /translation="MTLDRTATITVVLLLGGALLLAVLNLATPAGSPLHVPTYAVGLA
                     GKYLCYAMLALAVDLVWGFAGILTLGHAAFFALGGYAMGMYLMREIGARGVYANPVLP
                     DFMVFLGWHHLPWYWYGFDWFPVALVMVVVVPAVLALVFGWLAFRSRVTGVYLSIMTQ
                     ALTYALMLAFFRNDMGFGGNNGMTDFKDIIGLPIQTAGTRCGLMVISAAFVCAQYLLA
                     RFIVGSRYGKVLIAVRDAESRTRFLGYRPESYKLVVFVLSAVMAGIGGALYVPQIGII
                     NPSEFSPGNSIEAVIWVAVGGRGTLVGAILGALLVNLGKTWFTSALPEVWLYALGGLF
                     IFTTLLLPRGLMGLFAPRSRLTPPEPAPDIPAGAATEIVGDVHEVTG"
     gene            1892..2638
                     /gene="urtD"
                     /locus_tag="VFN42_03890"
     CDS             1892..2638
                     /gene="urtD"
                     /locus_tag="VFN42_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HET8995787.1"
                     /translation="MTEAGTLLYLDGVTVSFDGLRALNELSLVLEPGEMRAVIGPNGA
                     GKTTMMDVITGKTRPDEGRVVFGAHTDLTRMDEPAIAALGIGRKFQKPTVFESHSVWD
                     NLLLSLAEDRRPRFTLFARETPSERRRVEEILVTTRLTEHRHRMAADLSHGQKQWLEI
                     GMLLAQEPQLLLVDEPVAGMTDAETEQTAELLREINRTRSVVVVEHDMAFVRALDVKV
                     TVLHEGSVLSEGSIDHVSADPRVIEVYLGR"
     gene            2642..>2918
                     /locus_tag="VFN42_03895"
     CDS             2642..>2918
                     /locus_tag="VFN42_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014457460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8995788.1"
                     /translation="MLHVDRVDLHYGAAIALRGVSITAQPGSVTCLLGRNGVGKTSLL
                     RAIVGAHPISKGAIRWEGAEISRLPTYERARRGIAWVPQGRDIFPLLT"
BASE COUNT          416 a          926 c         1044 g          532 t
ORIGIN      
        1 ccggccgctg gagacgctgc tggcgacctg gggcctgtcg ctggtgctgc agcaggcggt
       61 gcgctcgatc ttcggaccaa ccaaccgcga cgtggacacg ccgtggtgga tgagcggcga
      121 tacgcatctg ggcgggctga cactgacctg gaaccgcatg tacatcatgc tgttcgccgc
      181 gctggtgctg gcggcgctga tggcggcgtt gcgctacacg ccgctgggat tgcgcatgcg
      241 cgcggtgaca cagaaccggc ggatggccgc ggcgatgggc atccgcacgc cgtggatcga
      301 cgcgctgacc ttcgggctgg gctccggcgt ggccggcatc gccggcgtgg cgctgagcca
      361 aatcgacaac gtctcgccca atctcgggca gaactacatc atcgacagct tcatggtggt
      421 ggtgttcggc ggcgtcggca atctgtgggg caccgtgttc gcggcgatga ccctgggcat
      481 cgtcaacaag ttcctggagc ccttcgccgg cgcggtgctg ggcaagatcg tcgtgctggt
      541 gctgctgatc ctgttcatcc agcgcaagcc gcgcggcatg ttcccgttga agggccgggc
      601 ggtggaggca tgatgcggta ttgcgtcatg gccgggcttg acccggccat ctacccggcg
      661 ccgatcgggc aaccggactg ccgcgtcccg atcgttccgg ggcagatggc cgggtcaggc
      721 ccggccatga cacactgctg ggtgtcgtga tgactctcga ccgtaccgcc accatcaccg
      781 tggtcctgct gctcggcggc gcgctgctgc tggcggtgtt gaacctggcg acccccgcgg
      841 gctcgccgct gcatgtcccc acctacgcgg tggggctcgc cggcaaatac ctgtgctacg
      901 ccatgctcgc cttggcggtg gacctggtct ggggattcgc cggcatcctc accctggggc
      961 atgccgcgtt cttcgccctc ggcggctacg cgatgggcat gtatctgatg cgcgagatcg
     1021 gcgcgcgcgg cgtctatgcc aatccggtgc tgcccgactt catggtgttc ctcggctggc
     1081 atcatctgcc ctggtactgg tacggcttcg actggttccc ggtcgcgctg gtgatggtgg
     1141 tggtggtgcc cgccgtgctg gcgctggtgt tcggctggct ggcgttccgc tcccgcgtca
     1201 ccggcgtgta cctgtcgatc atgacccagg cgctgaccta tgcgctgatg ctggcgttct
     1261 ttcgcaacga catggggttc ggcggcaata acgggatgac cgacttcaag gacatcatcg
     1321 gcttgccgat ccagaccgcc ggcacgcgtt gcgggctgat ggtgatctcc gccgccttcg
     1381 tctgcgcgca atatctgctg gcgcgattca tcgtcggctc gcgctacggc aaggtgctga
     1441 tcgccgtgcg cgatgcggaa tcgcgcacgc ggttcctggg ctatcggccg gaatcgtaca
     1501 agctggtggt gttcgtgctg tccgcggtga tggcggggat cggcggggcg ctgtacgtgc
     1561 cgcagatcgg catcatcaat cccagcgagt tctcgcctgg caattcgatc gaggcggtga
     1621 tctgggtcgc ggtcggcgga cgcggcacgc tggtcggcgc catcctgggc gcgctgctgg
     1681 tgaacctggg caagacctgg ttcaccagcg ccttgccgga ggtctggctg tacgcgttgg
     1741 gcgggctgtt catcttcacc acgctgctgc tgccgcgcgg gctgatgggg ctgttcgcgc
     1801 cgcgcagccg gctgacgccg ccggaaccgg cacccgacat cccggccggt gcggcaaccg
     1861 agatcgtggg cgacgtgcat gaggtgacgg gatgaccgaa gcgggcacgc tgctgtacct
     1921 ggatggcgtc accgtcagct tcgacgggct ccgcgcgctg aacgaattgt cgctggtgct
     1981 ggaacccggt gagatgcgcg cggtgatcgg cccgaacggc gccggcaaga ccacgatgat
     2041 ggacgtgatc accggcaaga cgcggcccga cgaaggccgc gtggtgttcg gcgcgcatac
     2101 cgacctgacg cgcatggatg agccggcgat cgccgcgctg ggcatcggcc gcaagttcca
     2161 gaagccgacg gtgttcgaat cgcactcggt ctgggacaat ctgctgctgt cgctggcgga
     2221 agaccgccgc cctcgcttca ccctgttcgc gcgcgagacg cccagcgagc gccgccgcgt
     2281 cgaggaaatc ctggtcacca cgcgcctgac cgaacatcgc caccgcatgg cggccgacct
     2341 gtcgcacggg cagaagcaat ggctggaaat cggcatgctg ctggcacagg aaccgcagtt
     2401 gctgctggtc gatgaacccg tcgccggcat gaccgacgcg gaaaccgaac agaccgcgga
     2461 gctgctgcgg gagatcaacc gcacccgcag cgtcgtggtg gtggagcacg acatggcgtt
     2521 tgtgcgggcg ctcgacgtga aggtgaccgt gctgcacgag ggatcggtgc tgtcggaggg
     2581 ctcgatcgac catgtcagcg ccgatccgcg ggtgatcgaa gtgtatctgg gccgctgagc
     2641 gatgctgcac gttgaccggg ttgatctgca ttacggcgcc gccatcgcat tgcgcggggt
     2701 gtccatcacc gcgcagccgg gcagcgtcac ctgtctgctg gggcgcaacg gcgtggggaa
     2761 gacctcgctg ttgcgggcga tcgtcggtgc gcatccgatc agcaaggggg cgatccgctg
     2821 ggagggcgcg gagatttccc ggttgccgac ttacgagcgc gcgcgccgcg gcatcgcctg
     2881 ggtgccgcag ggccgcgaca tcttcccgct gctgacgg
//
LOCUS       DASSZH010000182         3567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1180250, whole genome shotgun sequence.
ACCESSION   DASSZH010000182 DASSZH010000000
VERSION     DASSZH010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180250"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..554)
                     /gene="tsaA"
                     /locus_tag="VFN42_03900"
     CDS             complement(84..554)
                     /gene="tsaA"
                     /locus_tag="VFN42_03900"
                     /inference="COORDINATES: protein motif:HMM:NF014082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HET8995789.1"
                     /translation="MANHPDQTDTIRPGEVTTALPAATDAGIYFIGTIRTPWHVRSEC
                     PKRGSLDGPLCTIVVDERWRAALAEIGQHARLQVLYWMHQARRDLVLQSRRGSTQATG
                     TFALRSPVRPNPIASSVVALVAVNGGTLTVRGLDCVDGTPLVDLKPYRPEPAGQ"
     gene            complement(612..1409)
                     /locus_tag="VFN42_03905"
     CDS             complement(612..1409)
                     /locus_tag="VFN42_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014106446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET8995790.1"
                     /translation="MTGRDRGADLAPIGNAPVVILVRPQLADNIGACARAMANGGLFH
                     LRLVAPRDGWPQEKAWRTASGADRLLEAATVHPTLADATADLHHVFATCPRPRHIIKP
                     VLTARGAGVELREICGRGLRAGLLFGPERAGLDNDDMAQADALIRYPLNPAFMSLNLA
                     QAVMVMAYEWWSAADPTPPRALMTNETRVATKGELDNFLTHLVDQLDACGFLRNLPKR
                     PGMVRNIRHFFQRGEVTEQELRTLHGVVTELAIGRRRRGRLERDDEA"
     gene            complement(1431..1505)
                     /locus_tag="VFN42_03910"
     tRNA            complement(1431..1505)
                     /locus_tag="VFN42_03910"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1471..1473),aa:Val,seq:tac)
     gene            complement(1559..1822)
                     /locus_tag="VFN42_03915"
     CDS             complement(1559..1822)
                     /locus_tag="VFN42_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET8995791.1"
                     /translation="MDLIAAVAEETELSRAKAAEVVDAVFGAIEKALKKQEEVRLVGF
                     GTFVTAERKAGTGRNPRTGEEMEIPASTTVRFKAGKGLKDAVQ"
     gene            complement(2061..>3567)
                     /gene="lon"
                     /locus_tag="VFN42_03920"
     CDS             complement(2061..>3567)
                     /gene="lon"
                     /locus_tag="VFN42_03920"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003620744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET8995792.1"
                     /translation="PWKKRSKIRNDVVAAEKVLNADHYGLDKVKERIVEYLAVQARAP
                     KVRGPILCLVGPPGVGKTSLGKSIAKATGRNFVRMSLGGVRDEAEVRGHRRTYIGSMP
                     GKVIQGMKKAKTSNPLFLLDEVDKLGADWRGDPSSALLEVLDPEQNSTFNDHYLEVDY
                     DLSDVMFVTTANSLRMPQPLMDRMEIIRIPGYTEDEKVEIAKRHLIAKQSEANGLKPD
                     EWHISDDALRDLIRYYTREAGVRNLEREIANLGRKSVKELVTNKQTKKVAITRKNLEK
                     FAGVKKFRFGETEAEDMVGVVTGLAWTEVGGEILTIESVMLPGKGAVKQTGKLGDVMQ
                     ESVTAALSYVRSRSIKFGIKPTLFEKRDIHVHVPEGATPKDGPSAGIAMATSIVSVLT
                     GIPVRRDVAMTGEITLRGRVLPIGGLKEKLLAALRAGITTFFIPNENEKDLAEIPDNV
                     KKNLKLIPVADVDEVIAQALARKPEAIEWQEPEEAPPPSKAAEPVTASLPH"
BASE COUNT          518 a         1227 c         1189 g          633 t
ORIGIN      
        1 gacgacatcc ggagcaaatc ggcggagacc atgcgcgccg cgttgctcgg ccccgtgccg
       61 cagaacgtca tccggcccga ggattactga cccgccggct cggggcggta cggcttcagg
      121 tcaaccagcg gcgtgccgtc cacgcagtcg agcccgcgca ccgtcagggt gccgccgttc
      181 acggcgacca gcgccaccac ggatgatgcg atcgggttcg gccgcaccgg cgagcgcagc
      241 gcgaacgtgc cggtggcctg ggtgctgccg cgccgggatt gcagcacaag gtcccggcgg
      301 gcctggtgca tccagtacag cacctggagc cgagcatgct ggccgatctc ggccagcgcc
      361 gcccgccagc gttcatccac cacgatggtg cagagcgggc catccaggct gccgcgcttc
      421 gggcattcgc tgcggacgtg ccagggcgtg cggatcgtgc cgatgaagta gatgccggca
      481 tcggtcgcgg ccggcagcgc ggtcgtgact tctcccgggc ggatcgtgtc ggtctggtcc
      541 ggatggttgg ccatctgcgg actgtgcggg caagccggtt ccgaagtcca gccgggcaac
      601 ggccgcggcg gtcaggcctc gtcgtcgcgc tccagccggc cgcgccggcg gcggccgatc
      661 gccagttcgg tgacgacgcc atgcagcgtg cgcagttcct gctcggtcac ctcgccgcgc
      721 tggaagaaat ggcggatgtt gcgcaccatg ccgggccgct tcggcaggtt acgcaggaag
      781 ccgcaggcat cgagctggtc caccaggtgc gtcaggaagt tatccagctc gcccttggtg
      841 gcgacacggg tctcgttggt catcagcgcg cgcggcggcg tcgggtcggc ggccgaccac
      901 cactcatagg ccatcaccat caccgcctgg gccaggttca gcgacatgaa agcagggttc
      961 agcggatagc ggatcagcgc gtcggcctgc gccatgtcgt cattgtccag cccggcacgc
     1021 tccggcccga acagcaggcc ggctcggagg ccgcggccgc agatctcccg cagttccacg
     1081 ccggcaccgc gcgcggtgag caccggcttg atgatgtgcc gcggtcgggg gcaggtggcg
     1141 aagacgtggt gcaggtcggc ggtcgcatcg gccagggtcg ggtgcacggt ggccgcttcc
     1201 agcagccggt cggcgccgga cgcggtccgc caggccttct cctgcggcca gccgtcgcgc
     1261 ggggcgacca ggcgcaggtg gaacagcccg ccattggcca tggcacgcgc acaggctccg
     1321 atgttgtccg ccagttgcgg gcggaccagg atcaccacgg gagcgttgcc gatcggcgcc
     1381 aggtcggcgc cccggtcacg gccggtcatg cgccgtgctg gctcctgcaa tggtgggcgg
     1441 tgacggtttc gaaccgccga ccctctcggt gtaaacgaga tgctctaccg ctgagctaac
     1501 cgcccgaaac cgcgcatggc cggtgaccgg caaagcagcg ccgctcaccg gactgtgatc
     1561 actgcaccgc gtctttcagg cccttcccgg ctttgaaacg aaccgtggtg gacgcgggga
     1621 tctccatctc ctcaccggtg cgcggattgc ggccggtgcc ggctttgcgt tccgcggtga
     1681 cgaaggtgcc gaagccgacc aggcgcactt cttcctgctt cttcagtgct ttctcgatcg
     1741 caccaaacac tgcatccacc acttccgccg ccttggcccg cgacagctcg gtctcctccg
     1801 caacggcagc gatgagatcc attttgttca cagctcaatc cttctcccga tgtcacgcat
     1861 gcccaacgct tgcgccgtcc cggcgatagc ggggggacac tatcggcgcg gtttcgcccg
     1921 cgtcaatcca gcgaaacggc ccgccagagg cttccggcag gccgttgaag tgctttaaca
     1981 ttccgatcgc gcaaaatcta cgcgagcccg tgcgaacccc tgcggtgaat ggccgagccg
     2041 gagctcggcc gtccacaggg tcagtgcggc aacgaggcgg tgaccggttc ggccgccttg
     2101 gacggcgggg gggcctcttc cggttcctgc cactcgatcg cctccggctt gcgggccagg
     2161 gcctgggcga tcacctcgtc cacgtccgcc accgggatca gcttcaggtt cttcttcacg
     2221 ttgtccggga tttccgccag gtccttctcg ttctcattag ggatgaaaaa ggtggtgatc
     2281 ccggcccgca gcgcggccag cagcttctcc ttcaggccgc cgatcggcag cacccgcccg
     2341 cgcagggtga tctcgccggt catcgccacg tcacgccgca ccgggatgcc ggtgagcacg
     2401 ctgacaatgg aggtggccat ggcgataccg gcggaaggtc cgtccttggg ggtggcaccc
     2461 tccggcacgt gcacgtggat gtcgcgcttc tcgaacaggg tcggcttgat gccgaacttg
     2521 atcgagcgcg accgcacgta gctgagtgcc gcggtgacgc tctcctgcat cacgtcgccc
     2581 agcttgccgg tctgcttcac cgcgccctta ccgggcagca tcaccgactc gatggtgagg
     2641 atctcgccgc cgacttccgt ccaggccagg ccggtgacca caccgaccat gtcctcggct
     2701 tccgtctcgc cgaagcggaa cttcttcacc ccggcgaact tctccaggtt cttgcgggtg
     2761 atcgcgacct tcttggtctg cttgttggtg accagttcct tcaccgactt gcggcccagg
     2821 ttggcgatct cgcgctccag gttccgcacg ccggcttccc gcgtgtagta gcggatcagg
     2881 tcgcgcagcg cgtcgtcgga aatgtgccac tcgtccggct tcaggccgtt ggcctcggac
     2941 tgcttggcga tcaggtggcg cttggcgatc tccaccttct catcctcggt gtagccgggg
     3001 atgcggatga tctccatgcg gtccatcagc ggctgcggca tgcgcaggct gttggccgtg
     3061 gtgacgaaca tcacgtcgga caggtcgtag tccacctcga gatagtggtc gttaaaggtg
     3121 gagttctgct ccgggtccag cacctcgagc agggcggagg acgggtcgcc gcgccagtcg
     3181 gcgcccagct tgtccacctc gtccagcagg aagagcgggt tggaggtctt cgccttcttc
     3241 atgccctgga tcaccttgcc gggcatggag ccgatatagg tccggcggtg gccgcggact
     3301 tccgcctcgt cccgcacgcc gcccagcgac atgcgcacga agttgcggcc ggtggctttg
     3361 gcgatcgact tgcccaggct ggtcttgccg acacccggcg ggccaaccag gcacaggatc
     3421 ggtccgcgca ccttcggcgc gcgcgcctgc accgccaggt actcgacgat gcgctccttc
     3481 accttgtcca ggccgtaatg gtcggcgttc agcaccttct cggcggcgac cacgtcgttg
     3541 cggatcttgc tgcgcttctt ccacggg
//
LOCUS       DASSZH010000183         3188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_328132, whole genome shotgun sequence.
ACCESSION   DASSZH010000183 DASSZH010000000
VERSION     DASSZH010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328132"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VFN42_03925"
     CDS             complement(<1..797)
                     /locus_tag="VFN42_03925"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HET8995793.1"
                     /translation="MSISIRPLHTLFAGEITGIDLTRPLTPGQTAAIEAALDHHGVVV
                     FPDQQLTDAQQLDFSRAFGELEITGGTGISKPGEQRLHPAFADVSNLEKGGRLLERDS
                     RRRLYSLGNMLWHSDSSFKAVPAKYSILSGRVVADQGGETEFADMRAAYDALDADTQT
                     VIEELVCEHSLLYSRALLGFTDLTEAERASMRPVRQRLVRTHPVTGRKSLYLASHIGS
                     IIGWPVPEARAFIIDLMEHATQRPFVYAHRWRPFDLVMWDNRVTMHRV"
     gene            1269..1718
                     /locus_tag="VFN42_03930"
     CDS             1269..1718
                     /locus_tag="VFN42_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET8995794.1"
                     /translation="MDAAPPSVAELAPVQPPAPPAHTAPPPAPPIRNDAATKVAATSG
                     LRVSFPPGKAELSPDSAAAIKHLVGSTQGNGAVTFTVAAYATGVADDPSVARRLSLSR
                     ALAVRTALLAAGVSASRIEVRAMGSNVHSGPLDRADVSVLGANDPAR"
     gene            1715..2857
                     /locus_tag="VFN42_03935"
     CDS             1715..2857
                     /locus_tag="VFN42_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotA"
                     /protein_id="HET8995795.1"
                     /translation="MTKPTTYLIRMVVFLVAVGSVVGVLSPRLIEIFDNNRVLNSLIL
                     VILLLGIVWNLRQVMRLSSEVNWLETFQTARSRLAAIPTPRLLAPMASMLAAREQHGR
                     NSQGRFTLSATSMRSLLDGIASRLDESRELSRYMIGLLIFLGLLGTFWGLLRTVNSVS
                     DVIGGMTLGSGDINQMFEHLKAGLAGPLAGMGTAFSASMYGLAGALVLGFLDLTAGQA
                     QNRFFNELEEWLAGQTRLGSGALSEGDTSVPVYVQALLEQTAENMEGLQHILSRGEDS
                     RLQANQAVLLLNERIGTLADTMRANQQLMLRIAETQAALAPALQRLSELHGGDDVARA
                     HLRNIEIYLQRLLADAEQGRAQSTAELRNDLRVLTRTVAALAEEPH"
     gene            2858..>3188
                     /locus_tag="VFN42_03940"
     CDS             2858..>3188
                     /locus_tag="VFN42_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995796.1"
                     /translation="MPGRSRRGGGGGLEAWPGYVDALSTLLMVTIFVLLVFVLAQGFM
                     SAALTGSNKALDKVNAQLAQLTNALSLERAHAGQLQLSIAQLNKQLGSSNAARDTLTQ
                     QLQAAQAQ"
BASE COUNT          470 a         1123 c         1095 g          500 t
ORIGIN      
        1 acgcggtgca tggtgacgcg gttgtcccac atcaccaggt cgaacgggcg ccagcgatgc
       61 gcatagacga acggtcgctg cgtcgcgtgc tccatcaggt ctatgatgaa ggcccgtgct
      121 tccggcaccg gccagccgat gatgctgccg atatgggacg cgaggtacag cgacttgcgc
      181 ccggtgacgg ggtgggtgcg caccaggcgc tgccgcacgg gacgcatgct ggcgcgctcc
      241 gcctcggtca gatcggtgaa gccgagcagc gcgcgggagt agagcaggga gtgttcgcag
      301 accagctcct cgatgacggt ttgcgtatcg gcgtccagcg cgtcgtacgc ggcgcgcatg
      361 tcggcgaact cggtttcgcc gccctgatcg gcgacgaccc ggccggacag gatggagtat
      421 ttcgccggca ccgctttgaa tgagctgtcg gagtgccaca gcatgttgcc caggctgtac
      481 agccgccggc ggctgtcacg ctccagcagc cggccgccct tttccaggtt ggacacgtcg
      541 gcgaaggcag gatgcagccg ctgttcgccg ggcttggaaa tccctgtccc gccggtgatc
      601 tccaactcgc cgaaggcgcg gctgaagtcg agctgctgtg cgtcggtcaa ttgctggtcg
      661 gggaacacca ccacaccatg gtgatccagc gccgcctcga tcgcggccgt ctgcccgggc
      721 gtcaacggtc tggtgaggtc gatgccggtg atctcgccgg caaacagcgt gtgcagggga
      781 cgaatgctga tgctcatggc gaaagcttac cgccgcgtaa atcgcccgtc acccgcgtcc
      841 tgcgggcggc ggtcgcgcaa cacgttttga ccggtcagcc cgattcacgc tccttcattg
      901 agcagcgagg atagctcggt tagggtgcga cccctatgcg ccattgtctg atcctttttg
      961 ccgctttgct tctgccggct gcgcccgcgg ttgcccaggt gacggtggat ttgcgtgcgc
     1021 tggacgcgct gccggctgtc ccgtccgcgc cagcgccgca tcgagccgtc ccgcagcggc
     1081 acgtacccag gccgatcgcc cgccccaaag ccgccgcagc tggcccgtcc cgtccggccc
     1141 cggtgacggc cagcgcccgg ccgtccgcgg ttccgccagc ggcaaagccg ccggaagcca
     1201 cggcggcgcc gacaaccgcg ccgccgccgg tcgctgccgc cggcgcggca gcgccgaaac
     1261 tgcccgcgat ggacgccgcg ccgccctcgg tggccgagct ggcaccggtc cagccgccgg
     1321 cgccgccggc ccacacggcg ccgcccccgg caccgccgat ccggaatgat gcggcaacca
     1381 aggtggccgc caccagcggg ctgcgcgtca gcttcccgcc gggcaaggcc gagctgagtc
     1441 ctgacagcgc cgcggcgatc aagcatctgg tcggctcgac ccaggggaat ggcgcggtca
     1501 cgttcacggt ggcggcctat gccaccggcg tggcggacga cccgtcggtc gcccggcgcc
     1561 tgtccctgtc ccgcgcgctg gcggtgcgca ccgccctgtt ggccgccggc gtcagcgcca
     1621 gccgtatcga ggtccgcgcc atgggttcca acgtccactc cggcccgctc gaccgtgccg
     1681 atgtctccgt gctcggcgcg aacgatcccg cacgatgacc aaacccacga cctacctcat
     1741 ccgcatggtg gtgtttctgg tcgcggtggg gtcggtggtc ggcgtcctct cgccgcgcct
     1801 gatcgagatc ttcgacaaca accgggtgct gaacagcctg atcctggtga tcctgttgct
     1861 gggcatcgtg tggaacctgc gccaggtgat gcggctcagt tccgaggtga actggctgga
     1921 gaccttccag accgcgcgca gccgcctcgc cgccatcccg acgccccggc tgctggcgcc
     1981 aatggccagc atgctggcgg cgcgcgagca gcatgggcgc aacagccagg gccgcttcac
     2041 cctgtccgcc acctcgatgc gcagcctgct ggacggcatc gcctcccgcc tggacgagag
     2101 ccgcgaactc tcccgctaca tgatcgggct gctgatcttc ctcggcctgc tcggcacgtt
     2161 ctgggggctg ctgcgcacgg tgaactcggt gtccgacgtg atcggcggca tgaccctggg
     2221 cagcggcgac atcaaccaga tgttcgagca cctgaaggcc gggctggccg ggccgctggc
     2281 cggcatggga acggcgttct cggcgtcgat gtacggcctg gccggcgcgc tggtgctagg
     2341 gttcctggat ctcaccgcgg gtcaggcgca gaaccgcttc ttcaacgaac tggaggagtg
     2401 gctggccggc cagacccgcc tgggctccgg cgcgctgtcg gagggcgaca cctcggtgcc
     2461 ggtctacgtt caggcgctgc tggagcagac cgcggagaac atggaaggcc tgcagcacat
     2521 cctgtcgcgc ggcgaggact cccgcttgca ggcgaaccag gccgtgctgc tgctgaacga
     2581 gcgtattggc acgctcgccg acaccatgcg cgccaaccag cagctcatgc tgcggatcgc
     2641 ggagacgcag gccgcgctgg cgcccgccct gcaacgcctc tccgaattgc acggcggcga
     2701 tgacgtggcg cgggcgcatc tgcgcaacat cgagatctac ctgcagcgcc tgctcgccga
     2761 cgcggagcag ggcagggcgc aatccaccgc ggaactgcgc aacgacctgc gcgtgctgac
     2821 ccgtaccgtc gcggcgctcg ccgaggagcc gcactaggtg cccgggcgtt cgcggcgcgg
     2881 cggcggcggc ggcctggaag cctggcccgg ctacgtggac gccctgtcca cgctgctgat
     2941 ggtcaccatc ttcgtgctgc tggtgttcgt gctggcacag ggtttcatgt cggcggcgct
     3001 gaccggcagc aacaaggcgc tggacaaggt gaacgcgcag ctggcccagc tgaccaacgc
     3061 gctgtcgctg gaacgcgccc atgccggcca gctgcaactc tccatcgcgc agctcaacaa
     3121 gcagcttggt agttccaacg cggcgcggga cacgctgacg cagcagctgc aggcggcaca
     3181 ggcgcagg
//
LOCUS       DASSZH010000184         2415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_106474, whole genome shotgun sequence.
ACCESSION   DASSZH010000184 DASSZH010000000
VERSION     DASSZH010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106474"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VFN42_03945"
     CDS             <1..284
                     /locus_tag="VFN42_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012812834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HET8995797.1"
                     /translation="VPEADKDRRLQALQTLLRDQQARFGASCVGLDMPVLFTGTGRHP
                     GQIVGRSPFLQPVHVSAGADLIGTESMVRIVAAHPNSLAGTLLQERRTA"
     gene            290..1351
                     /locus_tag="VFN42_03950"
     CDS             290..1351
                     /locus_tag="VFN42_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003620339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET8995798.1"
                     /translation="MAAPQAPTAPAQSGGKAITLQFNDNTLLPLLLGDHDRHLVRIEQ
                     ALGVRLSCRGNRVAIAGDSSRVDMAQEMLRGLWRRLERGESVGRAEVEAAIRLADADN
                     DPRLPLSDLPAIRTRRGAVGPRSAGQAAYMEALVNHEMVFGVGPAGTGKTFLAVAQAV
                     AMLQAGKVDRIVLSRPAVEAGERLGFLPGDMKEKIDPYLRPLYDALHDMMPGDQVIRR
                     MSNGEIEVAPLAFMRGRTLAHCYAILDEAQNTTAMQMKMFLTRMGEGTRMVITGDLSQ
                     VDLPSGVRSGLRDALDTLEGIQGIGVVRFDQRDVVRHPLVARIVGAYDQRAAAQGEAR
                     RERERPPTPERKMSDGTSQ"
     gene            1335..1787
                     /gene="ybeY"
                     /locus_tag="VFN42_03955"
     CDS             1335..1787
                     /gene="ybeY"
                     /locus_tag="VFN42_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003620340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET8995799.1"
                     /translation="MEPPSRQPPALDGDAVEIVVADPAWHRLIPRADAIARRAAYAAG
                     MSGTIVLGSDRMVRVLNARHRGRNKPTNVLTYEHPAPEIILALGVVRAEAAAQGRRPA
                     HHLAHLVVHGALHLDGEDHEHPGDARRMEMAESRILHRIGVPNPWKRA"
     gene            1784..>2415
                     /locus_tag="VFN42_03960"
     CDS             1784..>2415
                     /locus_tag="VFN42_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET8995800.1"
                     /translation="MSGHRSSFLSRLGFLLGRKHTEHSLRESIAELVQEAAEAQQVPG
                     EQPELDRHERLLIANVLRLRETTADDVMVPRADIVAMRADVTLAQAIELIRNDGHSRL
                     PVYREQLDDVIGMVHIKDVFAYVGRPEAFSLEAILRRPLMVAPQIPVLDLLLQMRQAR
                     IHLALVVDEYGGIDGLVTIEDLIEEIVGDIEDEHDVDETPTLIERPDGSI"
BASE COUNT          416 a          814 c          814 g          371 t
ORIGIN      
        1 aggtgccgga agccgacaag gaccgccggc tgcaggcgct gcaaaccctg ctgcgggacc
       61 agcaggcccg cttcggcgca agctgcgtcg ggctggacat gcctgttctg ttcaccggca
      121 ccggccgcca ccctgggcaa atcgtcggac gttcaccatt tcttcaaccg gttcacgtgt
      181 ccgctggcgc agaccttatt ggcactgaat cgatggtgcg aatcgttgcc gctcatccaa
      241 actcactggc aggaaccctt cttcaggaga gacgaacagc ttgacccaac tggccgcccc
      301 tcaggctccg accgcccccg cccaatccgg cggcaaggca atcaccctcc agttcaacga
      361 caacaccctg ctacccctgc tgcttgggga tcacgaccgc catctggtgc ggatcgagca
      421 ggctctgggg gtccggcttt cctgtcgagg caatcgggtg gccatcgccg gggactcgtc
      481 ccgggtcgat atggcgcagg aaatgctgcg cggcctgtgg cgccggctgg aacgcggcga
      541 aagcgtcggc agggcggagg tggaggcagc catccggctg gccgatgctg acaacgatcc
      601 acgcctgccg ctgtcggacc tgccggccat ccgcacccgc cgcggcgccg tcggcccgcg
      661 cagcgcgggg caggccgcct acatggaagc cctggtcaac catgagatgg tgttcggcgt
      721 cggccccgcc ggcaccggca agactttcct ggccgtggcc caggccgtcg ccatgctgca
      781 ggcgggcaag gtcgatcgca tcgtcctctc ccgacccgcg gttgaggccg gggagcgtct
      841 gggcttcctg cccggcgaca tgaaggaaaa gatcgatccg tatctgcgcc cgctgtatga
      901 cgcgctgcac gacatgatgc cgggcgacca ggtcatccgc cgcatgtcga acggggagat
      961 cgaggtggcg ccgctggcct tcatgcgcgg ccgcaccctg gcgcactgct acgcgatctt
     1021 ggatgaggcg cagaacacca ccgccatgca gatgaagatg ttcctgacgc gcatgggcga
     1081 ggggacgcgc atggtcatca ccggcgacct gagccaggtg gacctgccga gcggggtgcg
     1141 gtccggcctg cgcgacgcgc tggatacatt ggaaggcatc cagggaatcg gcgtcgtccg
     1201 cttcgatcag cgtgacgtcg tgcggcatcc tctggtagca aggatcgttg gtgcctatga
     1261 tcagcgcgcc gcagcacaag gagaggcacg ccgggagcgg gaacgccccc caacccccga
     1321 gcgtaaaatg agcgatggaa cctcccagta ggcagccgcc cgcactcgac ggcgacgctg
     1381 tcgagattgt cgtggcggac cccgcctggc acagactgat accgcgggcc gacgcgatcg
     1441 cccggcgggc agcatacgct gcaggcatgt ccgggacgat cgtcctgggc tccgaccgca
     1501 tggtgcgtgt cctgaacgcg cgccaccgcg gccggaacaa gccgaccaac gtcctgacct
     1561 acgaacatcc cgcgccggaa atcatcctgg cgctgggcgt cgtccgggcc gaggccgctg
     1621 cgcagggccg ccggccggcg catcatctgg cccacctggt tgtccatggc gccctgcacc
     1681 tggacggtga ggatcacgaa caccccggcg acgcccgccg gatggagatg gccgaatcac
     1741 gtatcttgca ccgcattggc gtgcccaacc cgtggaagcg cgcatgagcg gacaccgaag
     1801 cagcttcctt tcccgcctgg gtttcctgct tggccgcaag cacaccgagc attcgctgcg
     1861 cgaatccatc gccgaactgg tgcaggaggc cgccgaggcg cagcaagtgc ccggggagca
     1921 gccggaactc gatcgccacg agcggctgct gatcgccaac gtgctgcgcc tgcgcgaaac
     1981 caccgctgac gacgtgatgg tgccgcgggc ggatatcgtc gccatgcgcg ccgacgtgac
     2041 gctggcgcag gcgatcgagc tgatccgcaa tgacggtcac tcccgcctgc cggtttaccg
     2101 ggaacagttg gacgacgtga tcggcatggt ccacatcaag gacgtgttcg cctatgtcgg
     2161 ccggccggag gcgttctcgc tggaggcgat cctgcgccgg ccgctgatgg tggcgccgca
     2221 gatcccggtg ctcgacctgc tgttgcagat gcgccaggcg cgcatccatc tggcgctggt
     2281 ggtggacgag tacggcggca tcgacgggct ggtgacgatc gaggacctga tcgaggagat
     2341 cgtcggcgat atcgaggacg agcacgacgt cgacgagacg ccgacgctga tcgagcggcc
     2401 ggacggcagc atcat
//
LOCUS       DASSZH010000185         3252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_157691, whole genome shotgun sequence.
ACCESSION   DASSZH010000185 DASSZH010000000
VERSION     DASSZH010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157691"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VFN42_03965"
     CDS             complement(<1..186)
                     /locus_tag="VFN42_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995801.1"
                     /translation="MFVRPQRDDFPVLATYGTLVEFHCADVLFALEWSAVAPGVGGWD
                     VLIDDERHTRLISVVPPG"
     gene            complement(240..488)
                     /locus_tag="VFN42_03970"
     CDS             complement(240..488)
                     /locus_tag="VFN42_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995802.1"
                     /translation="MDIASELRRSAYHVASIIFVLALYCMLTGTEAAAALLGATGVAI
                     LVALITTGDHLSQLYPPRREAIRAGYSDADDLEEEHEW"
     gene            1220..2008
                     /locus_tag="VFN42_03975"
     CDS             1220..2008
                     /locus_tag="VFN42_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET8995803.1"
                     /translation="MQYHTDLPSIRDRVSNEEWQARVDLAACYRLVDLYEMTDLIYNH
                     ITVRVPGTEHLLINLYGLLYREITASSLAVIDVEGNIIWKPETGYGINQAGYVIHGAI
                     HKARPDVNCVIHTHTRAGMAVSSMKCGLLPLNQTAIRFHGHIGYHDYEGPAINLDERA
                     RLVESLGEHDALVLRSHGLLTCGPDIPQAFNTMYQLEMACRAQVDAMAARTELLIPGD
                     DVLERTAHLYRPGTRRPFGVLEWPAMLRLLEAEGRNSGFPPHTH"
     gene            2214..2459
                     /locus_tag="VFN42_03980"
                     /pseudo
     CDS             2214..2459
                     /locus_tag="VFN42_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            2581..2814
                     /locus_tag="VFN42_03985"
     CDS             2581..2814
                     /locus_tag="VFN42_03985"
                     /inference="COORDINATES: protein motif:HMM:NF015799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HET8995804.1"
                     /translation="MFRRHRQAQQDKAAAEGQLQERSVIDRAKAILIRERRLSEPDAY
                     RWRRRRAMSLGKRVVEVAAMLVRAHEEGGDVRF"
     gene            2801..>3252
                     /locus_tag="VFN42_03990"
     CDS             2801..>3252
                     /locus_tag="VFN42_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010667599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8995805.1"
                     /translation="MSASDAAPIRIGLLRLSDSAPVLLAEASGAFAQHGLDVQIVVEP
                     SWANIADKLAYGLLDAAVMPPPLALAAAAGLRGMPSGLAVAMGLSLGGNAVVLGGDAA
                     AALRGATEGGPLSAAGARGAGPARSGDRPAPHGDGGSRRSAPAARHRQR"
BASE COUNT          541 a         1052 c         1113 g          546 t
ORIGIN      
        1 acccggtggc acgaccgaga tcaaccgggt gtgtcgctca tcgtcgatca gcacgtccca
       61 gccgccgaca ccgggcgcga cggcagacca ttccagtgcg aacaagacgt ccgcacagtg
      121 gaattcgaca agcgttccgt acgtggcaag aaccgggaag tcgtcgcgct gcggccggac
      181 gaacatgatt cctctccttt aactgccggg gactcccggg ctcctgcgag ggcgacggtt
      241 caccactcgt gctcttcctc aagatcgtct gcatcggagt agccggccct gatcgcttcg
      301 cggcgcgggg gatagagctg cgacaaatga tcgcctgtgg ttatcagagc taccaggatc
      361 gcaaccccgg tcgcgccaag caaagccgcc gcggcctcgg tgccagtgag catgcaatag
      421 agggcgagca cgaagatgat gctggcgacg tggtaggccg agcggcgaag ttccgatgcg
      481 atgtccatgt tggtgaacct ccgatccacg cccgccaatg ctggtcatcc atcgtgtccg
      541 gttcttggca ggagcgtgtc acgatggaga tcaagcagcg ggcgtggtga cagtcgcgca
      601 gatcggcggc attggctgtg acctgcacca caggaggcca ttgcgtgcga cgtcccgccg
      661 caatcgccgg ccggtcgtct gcctcctcgg cccggccgcg cagcggggac ttgcggcgcg
      721 caaatctcgg caccatgtcc cccggcaaca tctcaacctt atggagaacg tgcgttgtcc
      781 cgcaaacttg ccttcgctga ccaccttcga aatgcgcgcg ttcggcgtgg gctgtccgta
      841 gcggaggttg ctgaacgtat tggtgtcagc acgtccgcga tttacttctg ggaatcggac
      901 cgggcgcggc cgcgcgacgc aaacctctcg gcactctgca aggtcctgaa gctgcccgtc
      961 agggccacgc gggagttggc ggccggatag cgcatggccg ccatgccgcc ccaagccagc
     1021 acgcctgacg gcagcgggcg ctcacccgcc ttgatttcga tgccgggaat cgtcgtgcgg
     1081 tgcgccaggg ggctgtgaca ggtcctgcat cggcatccat gacggagcgg tctgggcagc
     1141 ggtctctttg acgcggacag gagcgccttc cccaatcaag aatgaatgcc gcgcaacagt
     1201 gggcctggaa ggagacgcca tgcaatatca taccgatttg ccctcgattc gcgaccgcgt
     1261 ttcaaatgag gagtggcagg cgcgtgtcga tctggcggcc tgctatcgcc tggtcgacct
     1321 gtacgagatg acagacctta tctataatca tattacggtt cgtgttcccg gcacggagca
     1381 cctgctcatc aacctgtacg gactgctcta cagggagatc accgcctcaa gcctcgccgt
     1441 gatcgatgtg gaggggaata tcatctggaa gccggaaacc ggctacggca tcaatcaagc
     1501 cggctacgtg atccatggcg cgatccacaa ggcacggccg gatgtgaatt gcgtgatcca
     1561 cacccacacc cgtgccggga tggcggtatc gtcgatgaaa tgcggtctgc tgccgctcaa
     1621 tcaaaccgcg atccggtttc acggccatat cggctatcac gattatgaag ggccggcgat
     1681 caatctcgac gagcgcgcgc ggctggtgga aagcctcggt gagcacgacg cgctggtcct
     1741 gcgctcgcac gggctgctga cctgcgggcc tgatatcccg caggcattca acaccatgta
     1801 tcagcttgag atggcatgcc gcgcccaggt ggacgcgatg gcggcccgaa ccgaactcct
     1861 gatcccgggc gacgacgtgc tggaacgcac cgcccacctc taccgcccgg gcacgcggcg
     1921 ccccttcggc gtgctggaat ggcccgcgat gctgcggctg ttggaggccg aggggaggaa
     1981 ttccggcttc ccgccccata cgcactgagg gcgttcgcag ggcgtctgcc cccgcagcca
     2041 tcaacggatc aagggcgatc cgttcacgaa gccgttggaa tgcggagtcc ccgtgcggga
     2101 cgtcgggcgg tccggagacc tccgcatgtc gttccgacgg atgacccgtc atgccgccgc
     2161 cccggcggtg ctggcccgca acttgccatg ccttccggca tgccgcagcc aagatgtttg
     2221 atggcgtgaa agtgttgctg gccgacaacg acgccgagcg ggcgcgcgcg ctggccgaaa
     2281 cgctcgcccg cgatccggtg ctgcaggtcc gccgcctccc gcacgatgca agcctgcccg
     2341 aagcggtaac cgagcacgcg ccggacgtgg tgatcgtgga catggcgcgc ccggatcgcg
     2401 acgcgctgga gggtgtgcgg caggtgacgg cggcggagcc gcggccgatc gtgctgttcg
     2461 tcgaccagga cgatcccgcc ttcatggagg atgcgatcga tgccggtgtg tcgtcctaca
     2521 acgtgctcgg tacgccgccg ccgccggacg tgaagccgat cctgcgcgtc agccgttgcc
     2581 atgttccgcc gtcatcgcca ggcgcagcag gacaaggcag cggcggaagg ccagttgcag
     2641 gaacggagcg tcatcgaccg cgccaaagcc atcctgatcc gcgaacgccg cctgtcggag
     2701 ccggatgcct atcgctggcg gcgccgccgg gcgatgtcgc tgggcaagcg ggtggtggag
     2761 gtggcggcga tgctggtgcg cgcgcatgag gagggtggcg atgtccgctt ctgacgcggc
     2821 gcccatccgc atcggcctgc tgcgtctgag cgacagtgcc ccggtgctgc tggcggaggc
     2881 gtcgggcgcc ttcgcgcagc atggtcttga cgtgcagatc gtggtcgaac cgtcctgggc
     2941 caatatcgcc gacaagctgg cgtacgggtt gctggacgcg gcggtgatgc cgccgccgct
     3001 ggcgcttgcc gcggcggcgg ggctgcgcgg catgccgtcg gggcttgccg tggcgatggg
     3061 actgagtctc ggcggcaatg cggtcgtcct ggggggcgat gccgccgcgg ccctgcgtgg
     3121 cgcgacggag ggcgggccgc tgtccgccgc cggcgcgcgc ggcgcgggac ctgcgcgtag
     3181 tggtgatcga cccgcgccgc acggcgacgg cggaagccgc cgatctgcac ctgccgctcg
     3241 ccatcggcag cg
//
LOCUS       DASSZH010000186         6645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1401860, whole genome shotgun sequence.
ACCESSION   DASSZH010000186 DASSZH010000000
VERSION     DASSZH010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6645
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401860"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VFN42_03995"
     CDS             <1..314
                     /locus_tag="VFN42_03995"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HET8995806.1"
                     /translation="MVVVGLGGILVEVLQDTALAPAPVTPADALEMLRSLKGVKLLQG
                     FRGMPAVDMPRLAAVISNVSRFAADQRAVLAELDVNPLICAGDRITAVDALIVPAGKA
                     G"
     gene            387..2723
                     /locus_tag="VFN42_04000"
     CDS             387..2723
                     /locus_tag="VFN42_04000"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HET8995807.1"
                     /translation="MTDPTTRAYTLPGLEHPAEILVDRWGIPHIRAESQHDVFFVQGF
                     NAARDRLYQIDLWRKRGLGQMAADYGPGFLAQDRAARLFLYRGDMAAEWAAYGNPNAQ
                     AICAAFAGGINAYIALAEQDPSLLPPEFVAMDIRPARWQPEDVVRIRSHALCRNVESE
                     VLRARVAAHATLDTDRARRSIEPPWDLRLPEGLDPADIPAEVLDDYWLATVDPDISPE
                     RLAATPDSAWRWTKVNDFDEVTQDAAAEGSNNWVIAPTRTATGRPILASDPHRTYQMP
                     SVRSVVHLTCPGMDVIGATEPPTPGVFIGHNDRLAFGLTIFPMDQEDLYVYQTHPDDP
                     DQYRYGDGWERMRVVEEQVAVRGMPPQTVTLKFTRHGPVVHADAAKHRAFAVRSVWSE
                     PGTSAYMGRLAYMTAQSVQQHGEALRHWSAPTVNQISADLDGNIGWFAVGKAPRRPNW
                     DGLLPVPGDGRYEWDGFHPLEDLPRRINPPRGFVATANEMNMPPDYPALQRKLGFEWA
                     ERSRSNRVHAVLDAQPQHDIADSMALQCDVFSIPAQRIGRLLDRITNTPAGLETALAM
                     LRGWDHQLRADSAAAALFEVWWVKHLKSALLNRVSSDPTVRPLLVPGDNETLLAMLEA
                     AEPASLGTLLADTLGAAFTQCRSLLGDDPATWRWGRLHHGYFTHPLSRVAGQHGLRDV
                     GPLPLGGSGSTPMNTTYRATNFRATVGASFRMVVDVGNWDNSRVINTPGQSGNPTSPH
                     YDDMAPLWARGEYVPMLYSREAVDSAAELRIVLRPEKA"
     gene            complement(2754..5387)
                     /locus_tag="VFN42_04005"
     CDS             complement(2754..5387)
                     /locus_tag="VFN42_04005"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HET8995808.1"
                     /translation="MTQSPRLTQLRLTFIVVLAGLTMTVIGGWIALSADRQAIRRELR
                     GSAELHAKIIEARFSELEHPVLTAADFIAASLDHNPTEFLRFTRFAAPHAPIVQWLGW
                     APLVPDADRAAFETTARARYPDFQIADRSGNALVPAGTRSVHFPLYAARDFRGRSVPL
                     GLDLAGDPVLRKTAERARDEGRAVATGPLPSLDGGKSGEITILVAPIYRNGITALRGV
                     AARQNSLIGFIVGSYGVDAMLGFVTAGVVAGDETVSFTRPPPARRLGAAIAATPAGMR
                     SVLTPEPFASLARDGYRFVQPLTFSGQVWTATFTFPSQAVAQRSSYAPFGWLGAGLLL
                     TMALGTITARGESRKLAVERLVATRTQELSSANERLRQSAETLEATLAAAPLGIIALD
                     PEATIVQWNRGAERIFGYAATEVLGKPYFPLMVPQGHRTEADALFLRACNGERLRDVA
                     VQRQRRDGMLLDIRVAGNPLHDAHGRLRGVVFANQDVTESLRLEEQLRQAQKMEAIGQ
                     LTGGVAHDFNNLLGTAITSLDLLRDRIRHDAEGSDLAADALDALLHGADLVRRLLAFA
                     RRQPLQPEHLRLNVLVLEAVRLLRPLLGDAIEISLDLGSDLWPVLVDRAQFETTLINL
                     ATNGRDAMPHGGQLRIVTYNLCVTAQAPARNLEVSPGEYVVVEVSDTGTGMPPALLQR
                     IFEPFFTTKGKSVGTGLGLSTVIGFIKQSGGHIDVESEPGRGSCFRLYLSRHVDDAPA
                     ISVPDAAAYTRPPSATQGERVLVVEDNAGLRHSVLHQLKKFGYQTMEAEDAAGALAAL
                     ESGGPVDLLFTDVMLPGGMTGVELATTARQRWPDLKVLLTSGFPEALLDEPSSLDGLK
                     LIGKPYREEELARTLREVLAG"
     gene            5596..>6645
                     /locus_tag="VFN42_04010"
     CDS             5596..>6645
                     /locus_tag="VFN42_04010"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8995809.1"
                     /translation="MPTFSTAAESRRALPRELIRRLPFFYGWVILGCVCCAGFARQGG
                     AVATLSIFVEPMTRHFGWSRTAISGAVSLGGLLAALASPLLGRLLDRQGGRVVLGLAV
                     LLTGAADLGLSLTGSLAMFYALFCVARMNFAGPFDLGIYGAVNNWFVSRRTLAISIAT
                     VAQMTGLVALPLIAEAMILRGGWRAGWLGVGIAVLAVGFIPNWLFMVRRPEDVGLLPD
                     WPARSSATAVAHVEPDFTRAQALRTPAFWLLSLFTLLAYPVQAGVSLHQAPFLIERGI
                     SPAIAASIVSVFSMMSGVASLGFGFYPRRLPVRFALALVGGLLCASTLAMLSVHSAAV
                     GFLAASVFGLGIGGLL"
BASE COUNT         1031 a         2210 c         2253 g         1151 t
ORIGIN      
        1 cgatggtggt ggtcggcctg ggtggcatcc tggtggaggt gctgcaggac accgcgctgg
       61 cgcccgcgcc ggtaaccccg gcggatgcgc tggaaatgct gcgctcgctg aagggcgtga
      121 agctgctgca aggcttccgc ggcatgccgg cggtggacat gccgcgactg gccgcggtga
      181 tcagcaatgt ctcgcggttc gccgcggatc agcgggcggt tttggcggag ctggacgtca
      241 acccgctgat ctgcgccggc gaccggatca ccgcggtgga cgcgctgatc gtcccggctg
      301 gaaaagcggg ataagagaga aaagccccct cgtttggcag gggccatcgt cctgctctgc
      361 gtcccgtccg atctggagtc tctcgcatga ccgacccgac gactcgtgcc tacacgctgc
      421 ccggcctgga acacccggcc gaaatcctcg tggaccgctg gggtattccg catattcgcg
      481 cggagtcgca gcacgacgtg ttcttcgtgc aggggttcaa cgccgcgcgt gaccggctgt
      541 atcagatcga cctgtggcgc aagcgcggtc tcggacagat ggccgcggat tacggcccgg
      601 gcttcctggc gcaggaccgc gccgctcggc tgttcctgta tcgcggggac atggcggcgg
      661 agtgggcggc ctacggcaat cccaacgcgc aggcgatctg cgccgccttc gccggcggga
      721 tcaacgccta tatcgcgctg gccgagcagg acccatcgct gctgccgccg gaattcgtcg
      781 ccatggacat caggccggcg cgttggcagc cggaggacgt ggtgcggatc cgcagccatg
      841 cgctgtgccg caatgtcgaa tccgaggtgc tgcgcgcccg ggtcgccgcg catgcgacac
      901 tggacaccga ccgtgcgcgg cgttcgatcg agccgccctg ggatctgcgc ctgccggaag
      961 gcctggaccc ggcggatatc ccggccgagg tgctggatga ttattggctg gcgacggtgg
     1021 atcccgacat cagcccggag cggctggccg ccacgccgga ctccgcctgg cgctggacca
     1081 aggtgaatga tttcgacgag gtgacgcagg acgctgccgc ggaaggctcc aacaactggg
     1141 tgatcgcacc aacgcgcacg gcgaccggcc gccccatcct ggcgtccgat ccgcaccgga
     1201 cctaccagat gccatcggtg cgttccgtgg tgcatctgac ctgccccggc atggacgtga
     1261 tcggcgccac cgaaccgccg acccctggcg tgttcattgg ccataacgac cgcctggcct
     1321 tcggcctgac cattttcccg atggatcagg aagacctgta cgtctaccaa acgcatcctg
     1381 acgacccgga ccagtatcgc tacggcgatg gctgggagcg catgcgagtc gtcgaggagc
     1441 aggtcgcggt gcgcggcatg ccgccgcaga ccgtgacgct gaaattcacc cgccacggcc
     1501 cggtggtgca cgcggatgcc gcgaagcatc gtgcctttgc cgtgcgctcg gtgtggtccg
     1561 agccgggtac ctccgcctat atgggccggc tggcctacat gaccgcccaa tcggtgcaac
     1621 agcatggcga ggcgctgcgc cattggtcgg cgcccacggt gaaccagatt tcggcagacc
     1681 tggacggcaa tatcggctgg ttcgctgtcg gcaaagcgcc gcgccggccg aactgggacg
     1741 gtctgctgcc ggttcctggc gacggccgtt atgaatggga cgggtttcac ccgctggaag
     1801 acctgccgcg ccggatcaac ccgccgcgcg ggttcgtcgc gacagccaac gaaatgaaca
     1861 tgccgccgga ctatcccgcc ctgcagcgca agctcggctt cgagtgggcg gagcggtcgc
     1921 gctccaatcg cgtccatgcc gtgctcgatg cacagccgca gcatgacatc gccgattcga
     1981 tggcactgca atgcgacgtg ttttccatcc ctgcgcaacg gatcggccgc ctgcttgacc
     2041 gcatcacgaa tacaccggcc ggactggaga ctgcgctcgc catgctgcgc ggctgggatc
     2101 accagctacg tgccgactca gccgcggcgg cattgtttga ggtctggtgg gtgaagcatc
     2161 tgaagtcggc gctgttgaac cgcgtgtcat ccgacccgac cgtgcgccca ttgctggtgc
     2221 cgggcgacaa tgaaacgctg cttgccatgt tggaggctgc ggaacccgcg agtcttggca
     2281 ctctgctggc tgacacgctg ggggctgcct tcacgcaatg ccggtcactg ctgggcgacg
     2341 atcccgcgac ctggcgatgg ggccggctgc atcatggcta cttcacgcat ccgctgtcgc
     2401 gcgtggcggg gcaacatgga ttgcgcgatg tcggtccgct gccgctcggc ggttcgggct
     2461 cgacgccgat gaacaccacc taccgcgcga ccaacttccg tgcgacggtg ggagcgtctt
     2521 tccgcatggt cgtggatgtc ggcaactggg acaacagccg cgtgatcaac acacccggcc
     2581 agtccggcaa tccaacctcg ccacactacg acgacatggc accgctatgg gcgcggggcg
     2641 agtacgtgcc catgctgtac tcgcgggaag cggtcgattc ggcggccgaa ctgcgcatcg
     2701 tgctgcggcc ggagaaagcc tgacgcaagg ccggagctgc ctcgccgcag gcactatccg
     2761 gccagcacct cgcgcaaggt gcgggcgagc tcttcctcgc ggtacggctt gccgatcagc
     2821 ttcaggccat ccagcgatga gggctcgtcc agcagcgctt ccggaaaccc ggaggtcagc
     2881 agcaccttca gatctggcca gcgctggcgt gcggtggttg ccagttcgac tccggtcatt
     2941 ccgcccggca gcatgacgtc ggtgaacagc agatccactg gtccgccgct ttccagtgcc
     3001 gccagtgcgc cggcggcatc ctctgcctcc atcgtctggt agccgaactt cttcagttga
     3061 tgcaggacgc tgtggcgcaa tccggcattg tcctccacca ccagcacgcg ttcgccttgc
     3121 gttgcgctgg gcggtctcgt gtacgcggcg gcatcgggca cgctgatcgc cggagcatca
     3181 tccacatggc gtgacagata gagccggaag cacgatcccc gccccggctc gctttccaca
     3241 tcgatatgcc cgccggactg cttgatgaag ccgatcaccg tgctcagtcc aagcccggtg
     3301 ccgaccgact tgcccttggt cgtgaagaag ggttcgaaga tccgctgcag cagcgccggc
     3361 ggcatgccgg tgccggtatc ggagacttcc accacgacat actcgcccgg actgacttcg
     3421 aggttgcgtg ccggagcctg agcggtgacg cacaggttgt aggtcacgat gcgcaattgc
     3481 ccgccatgcg gcatggcatc ccggccattg gtggccaggt tgatcagcgt cgtctcgaat
     3541 tgcgcgcggt ccaccagcac cggccataga tcgctgccaa gatccagcga aatctcgatc
     3601 gcatcgccca gcaacgggcg cagcagccgc acggcttcca gcaccagcac attcaggcgc
     3661 agatgttcgg gctgcaacgg ctggcgccgt gcaaaggcca gcagccgacg cacaaggtcg
     3721 gcaccgtgca gcagcgcgtc caatgcatcc gccgcaagat cggagccctc ggcatcgtgc
     3781 ctgatccggt cgcgcagcag gtcgaggctg gtgatcgcag tgccgagcag gttattgaaa
     3841 tcgtgtgcga caccgccggt gagttggccg atcgcctcca tcttctgggc ctgccgcaat
     3901 tgctcttcca ggcgcaggct ctcggtgacg tcctggttgg cgaacaccac gccgcgcaac
     3961 cgtccatgcg catcatgtaa cggatttccc gcgacgcgga tatccagcag catgccgtcg
     4021 cgccgctgcc gctgcacggc aacatcgcgc aggcgttcgc cgttgcaggc acgcaggaac
     4081 agcgcgtccg cttcggtgcg gtggccctgc ggcaccatca aggggaaata tggcttcccc
     4141 agcacctcgg tcgccgcata gccgaaaatc cgttcggccc cgcggttcca ttgcacgatg
     4201 gtcgcctcgg gatcgagcgc gatgatgccc agcggggccg ccgccagcgt tgcctccagc
     4261 gtttccgccg attgccgcag ccgttcgttg gcgctggaga gctcctgggt ccgcgttgcc
     4321 accaggcgct ccaccgcaag tttccggctc tcgccgcgag cggtgatggt gccgagggcc
     4381 atggtcagca gcaggccggc acccagccag ccgaacggcg cgtaggaaga acgctgtgcc
     4441 actgcctgcg agggaaacgt gaaggtcgca gtccagacct gcccggagaa ggtcagcggc
     4501 tggacgaagc gatagccgtc gcgtgccagc gatgcgaagg gttccggcgt gagcacggac
     4561 ctcatgccgg cgggtgtcgc cgcaattgcc gcgcccaacc gcctggcagg tggcgggcgg
     4621 gtgaagctga ccgtctcgtc gccggcgacg acacccgcgg tgacgaagcc cagcatggcg
     4681 tcaaccccgt agctgccgac aatgaagccg atcagactgt tctgtcgcgc tgcaacgccc
     4741 ctcagggcgg tgatgccgtt gcggtagatc ggggcgacca ggatggtgat ctcgcccgac
     4801 ttccccccat cgaggctggg gagcggaccg gtcgccacgg ctcggccttc atcgcgcgcc
     4861 cgttccgccg ttttgcgcag cactgggtcg cccgcgagat cgaggcccag cggcacgctg
     4921 cggccgcgaa aatcccgggc tgcgtacaag ggaaaatgca cggagcgcgt ccctgccgga
     4981 accagggcat tgccggaccg gtcggcgatc tggaagtccg gatagcgtgc gcgcgccgtc
     5041 gtctcgaagg cagcgcgatc cgcatccggc accagcggtg cccagccgag ccactgcacg
     5101 atcggcgcat gcggtgcggc aaagcgcgtg aagcgcagga attctgttgg gttgtgatcc
     5161 aggcttgcgg cgatgaagtc cgccgcggtc agcacgggat gctcaagttc ggaaaaccgc
     5221 gcttcgatga tcttggcatg cagctcggca ctgccgcgca gctcgcggcg gatcgcctgc
     5281 cggtcggcgg acagcgcgat ccatccgccg atgacggtca tcgtcaaccc ggccagcacg
     5341 acgatgaacg tgaggcgaag ttgcgtcagc cggggtgatt gcgtcattgc catccgcgtt
     5401 ctgcggcgcc gcggcagggc ggcaaaggcg ccgccagtcg cacggcaaag gggcgcgtgc
     5461 gctgatctgg atcaagcccg gtgggcgaaa tcgggaaaat gcatcaacgc ccattcggtc
     5521 cccgggaagg caggtttgcc gatgggccgg catggccgct atggctgtag cgtctccgca
     5581 cggttcccgt cccacatgcc gaccttctcc accgcagccg agtcccgtcg agccttgccg
     5641 agggagctga tccgccggct gccattcttc tatggctggg tcatcctggg ctgcgtctgc
     5701 tgcgccggtt tcgcacgcca gggtggcgcg gtcgccacgc tgtcgatttt cgtcgaaccg
     5761 atgacccggc atttcggctg gtcgcgcact gcgatttccg gcgcggtctc tcttggcgga
     5821 ctgctcgccg cgctcgcctc cccgctgctg ggcaggctgc tggaccgcca gggggggcgc
     5881 gttgtgctgg ggctggcggt gttgctgacc ggtgcggccg acctgggcct gtcgctgacc
     5941 ggctcgctgg cgatgttcta cgccttgttc tgcgtggcca ggatgaactt cgccggaccg
     6001 ttcgacctgg gaatctatgg cgcggtgaac aactggttcg tgtcgcgccg gacactggca
     6061 atctccatcg ccacagtggc ccagatgacc gggctggtgg cgctgccgct gatcgccgag
     6121 gcgatgatcc tgcggggcgg ctggcgcgcg ggttggctgg gtgtcggcat cgcggtgctg
     6181 gcggttggct tcataccgaa ctggctgttc atggtgcgcc ggccggagga tgtcggactg
     6241 ctcccggact ggccggcccg aagcagcgcc acggcggtgg cgcatgtgga accggacttc
     6301 acccgcgccc aggcgctacg cacgccggcc ttctggctgc tgtcgctgtt caccctgctg
     6361 gcctatccgg tgcaggcggg ggtcagcctg caccaggcgc cgttcctgat cgagcgtggc
     6421 atcagtcccg ccatagcggc cagcattgtc agcgtgttct cgatgatgtc cggggtggcc
     6481 agcctgggtt tcggcttcta cccgcgccgc ctgccggtgc gcttcgccct ggcattggtg
     6541 ggcgggctgc tctgcgccag cacgctggcg atgctgagcg tgcattcggc ggcggtcggt
     6601 ttcctcgcgg cgtcggtgtt cggcctgggc atcggcggcc tgctg
//
LOCUS       DASSZH010000187         2859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1436267, whole genome shotgun sequence.
ACCESSION   DASSZH010000187 DASSZH010000000
VERSION     DASSZH010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436267"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..317)
                     /gene="rpsO"
                     /locus_tag="VFN42_04015"
     CDS             complement(48..317)
                     /gene="rpsO"
                     /locus_tag="VFN42_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011253110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET8995810.1"
                     /translation="MSITAERRTALIGEYATGAADTGSPEVQVALLSERIGNLTEHLK
                     THAKDFHSRRGLLMLVGRRRRLLDYLKAKDHKRYEVLIGRLSLRR"
     gene            complement(484..1410)
                     /gene="truB"
                     /locus_tag="VFN42_04020"
     CDS             complement(484..1410)
                     /gene="truB"
                     /locus_tag="VFN42_04020"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010514730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET8995811.1"
                     /translation="MSRRKPRGRPLDGWLIIDKPAGMTSTDVVNRVKRVFDAQKAGHG
                     GTLDPLATGLLPVAFGAATKTVPYVMDGTKLYRFTLRFGEARDTDDADGAVTETSAVR
                     PADTQIQAALPAFRGAIMQVPPVFSAIKVAGERAYDMAREGRAPVLEPRPARVDRFEL
                     IARPDADTAVFEVASGKGVYMRSLARDLAQACGSVGHIAALRRLRVGPFHEQHAVTLD
                     KLRDADDTAPASARLLLPVATALADIPALALTEQEAADLRQGQPLSLIDLMGRIPPAA
                     DPAGGLARAMLGSRVVSLCRMEDGMLKPERTL"
     gene            complement(1591..>2859)
                     /locus_tag="VFN42_04025"
     CDS             complement(1591..>2859)
                     /locus_tag="VFN42_04025"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET8995812.1"
                     /translation="RRRGFPVGAVRVCQFRRSANIMAEIEEAFKHMSPAPLIDRELAT
                     AVSGDRIAGLVAVAANEHGVFYEAAFGRRNLALPQRMTVNSVFRIASMTKAITATAAM
                     QMVEQGKLSLDQPAQDILPFLAETKVLDGFDNAGRPILREPRGTITLRRLLTHTAGFV
                     YDTWNENMNRYAQLTGLPAARTGKLAALSAPLGFDPGERWEYGINIDIAGRMVEVVSG
                     LDLETYFQQHIFAPLGMVDTSFIERPDWENRLTAVHARQLDGTLEPIALPPGAAPDRE
                     FFPGGGGLYSTGSDYMRFLRALMHEGELDDHRILQPATVALMGQNQMGPLNVQPMRSA
                     IPRLSNDVDLFPGMVKKWGLSFLINTQPGPAGRRAGSLAWAGLNNTYYWMDPIRKVAG
                     VLMTQILPFADPTVLETLDAFERAVYATAA"
BASE COUNT          465 a          923 c         1008 g          463 t
ORIGIN      
        1 cacggcagcc ggggcaggga ccccggccgg tggtggcaga ggttgggcta acggcgcagg
       61 ctcaggcggc cgatcagcac ctcatagcgc ttgtggtcct tggccttgag gtaatcgagc
      121 aggcggcggc ggcggccgac cagcatcagc aggccgcggc gggagtggaa gtccttcgcg
      181 tgcgtcttca gatgctcggt caggttgccg atccgttcgc tgagtagagc gacctggact
      241 tcggggctgc cggtatcggc ggcgccggtc gcgtattcgc caatgagcgc cgtgcggcgt
      301 tcggccgtga tcgacatcgt gttttctcca ttcgacaggg tggtttgaca catggcccgg
      361 ccgggatgtc gtccagctgg gtcaggccca aggcggctgg gccgcaagga cgcggcggta
      421 tccgcttgtt gggccgcttt ggcaagcgaa aaccatgggg caagcgaaaa ccaggggccg
      481 ccgctacagc gtccgctccg gcttcagcat gccatcctcc atccggcaca atgacacgac
      541 ccggctgccc agcatggcgc gggcaaggcc accggccggg tccgccgcgg gcgggatgcg
      601 tcccatcagg tcgattaggc tgagcggctg gccctggcgc aggtcggcgg cctcctgctc
      661 cgtcaaggcc agggcgggga tgtcggccag ggcggtggcg acaggcagca gcagccgagc
      721 ggatgcgggc gcggtgtcgt ccgcgtcgcg cagcttgtcc agggtgacgg cgtgctgttc
      781 atggaagggg ccgacgcgca ggcggcgcag ggcggcgata tgcccgacgc tgccgcaggc
      841 ctgtgccagg tcgcgcgcca ggctgcgcat gtagacgccc ttgcccgagg ccacttcgaa
      901 caccgcggtg tcggcatcgg gacgggcgat cagctcaaac cggtcgacgc gggctgggcg
      961 cggttccagc accggggcgc ggccttcgcg ggccatgtcg taggcccgtt caccggccac
     1021 cttgatggcg gaaaacaccg gcggcacctg catgatggcg ccacgaaagg cgggcaatgc
     1081 ggcctggatc tgcgtgtccg ccggccgaac ggcgcttgtt tcggtcaccg cgccatcggc
     1141 gtcgtcggtg tcgcgggctt cgccgaaccg cagcgtgaag cggtacagct tggtgccgtc
     1201 catgacatag gggacagttt tggtggcggc gccgaacgcg accggcagca ggccggttgc
     1261 caatgggtcg agcgtgccac catgaccggc cttctgcgcg tcgaacacgc gcttgacgcg
     1321 gttcacaacg tccgtgctgg tcatgccggc cggcttgtcg atgatcaacc agccgtcgag
     1381 gggacggccg cgaggcttgc ggcgggacat ggatcggaac acccgggcga acaaagaggc
     1441 ggcgggttag ggactggggg cgctgttgtc aaccggattt acgggcacgg agcggcggcg
     1501 ccgtgcccgt cagcctgccg ggacctgtcg acctgccggt gaatgccgtc aggccaccgc
     1561 catcatgcca gtgctatcac cccaccggca tcacgccgca gtcgcgtata cggcccgttc
     1621 gaaggcatcc agcgtctcca gcaccgtagg atcggcaaac ggcaggatct gcgtcatcag
     1681 cacgccggcc accttgcgga tcggatccat ccagtaatac gtgttgttca gccctgccca
     1741 agccaggctg cccgctcggc ggccggccgg cccgggctgc gtgttgatca ggaaactcag
     1801 gccccatttc ttcaccatgc cagggaacag atcgacgtcg ttcgacaggc ggggaatcgc
     1861 cgagcgcatc ggctggacgt tcagcggacc catctggttc tggcccatca gcgcgaccgt
     1921 tgccggctgc aggatgcggt ggtcgtccag ttcgccctca tgcatcaggg cgcgcaggaa
     1981 ccgcatgtag tccgagccgg tggaatacag accgccacca ccgggaaaga actcccggtc
     2041 cggcgcggcg ccaggcggca gcgcgatcgg ctccagcgtg ccgtccagct ggcgggcgtg
     2101 caccgcggtc aggcggtttt cccagtccgg ccgctcaatg aagctggtat cgaccatgcc
     2161 cagcggggcg aagatgtgct gctggaaata ggtttccagg tccaggccgc tgacgacctc
     2221 caccatccgg ccggcgatgt cgatgttgat gccatactcc caccgctccc cgggatcgaa
     2281 gcccagcggg gcggacagag cagcgagctt gccggtgcgg gcggccggca ggccggtcag
     2341 ttgggcatac cggttcatgt tctcgttcca ggtgtcgtaa acgaacccgg cggtatgcgt
     2401 cagcaaccgg cgcagcgtga tcgtgccgcg cggttcgcgc aggatgggac ggccggcatt
     2461 gtcgaagccg tccagcacct tcgtctcggc caggaacggc aggatgtcct gcgccggctg
     2521 gtccagcgac agcttgcctt gctcgaccat ctgcatcgcc gcggtggcgg tgatcgcctt
     2581 ggtcatggac gcgatgcgga agaccgaatt caccgtcatg cgctgcggca gggcgaggtt
     2641 gcgcctgcca aacgcggctt catagaacac gccgtgctca ttggcagcga cggcgacgag
     2701 accggcgatc ctgtcaccgc tgacagcagt ggcgagttcc cgatcgatca acggggcagg
     2761 ggacatgtgt ttgaacgctt cctcaatctc ggccatgata ttggcgcttc tacgaaattg
     2821 gcaaacacgc accgcgccga ccggaaagcc ccgccgccg
//
LOCUS       DASSZH010000188         2952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_873618, whole genome shotgun sequence.
ACCESSION   DASSZH010000188 DASSZH010000000
VERSION     DASSZH010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873618"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..485)
                     /locus_tag="VFN42_04030"
                     /pseudo
     CDS             complement(36..485)
                     /locus_tag="VFN42_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019091236.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
     gene            complement(559..1860)
                     /gene="ftsA"
                     /locus_tag="VFN42_04035"
     CDS             complement(559..1860)
                     /gene="ftsA"
                     /locus_tag="VFN42_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET8995813.1"
                     /translation="MNDMSRRIVPPLTAPPEEPERPRHHRSGPFGVLDIGTTKIACLI
                     GRTESDGTLRVLGFGWHRGRGVRGGSIVDLDEAEKAVRACVGQAEDMADTRLRSVVVN
                     LSCGQPESRLFNVQWPVGGRAVGEADVRRVVNEGRARAVTEGRESIHALPLSFAVDET
                     EHVLDPRGLFCSTLGARLHIIDAASTALRSLGACMARCDLEISELVSAPMATGLSTLV
                     DDERELGCTIIDMGGGTTGMAVFAEGQLLHTAQLPIGGLHVTKDLATGLSTSINHAER
                     LKTLYGNVQACADDDRELLPVPLVGEEEHQIAKVPRSMVVNIIRPRLEETFEMVKDRL
                     DRSGLSRAAGNRVVLTGGACQLAGLREMAGRMLNRQVRLGKPHTVRGLPDSASGPAFA
                     TATGLLHWASGEGRTMHDMDLEEERPAGLLRRLVNFLKDHV"
     gene            complement(1876..2733)
                     /locus_tag="VFN42_04040"
     CDS             complement(1876..2733)
                     /locus_tag="VFN42_04040"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HET8995814.1"
                     /translation="MTRRTRNSVQDRPARTTLLLRRIRKFLRPVAWASAAMIALLMIA
                     GLVHSASPGGALATLRERLGNATAFAGLRVTHIVVKGRFNTPESALRTALGVNTGSPM
                     LGFSVEQARKRIEKLSWVDHATVERRLPGTVVVYLEERRPFAIWQHDGKFVLIDRNGQ
                     VVTNEDVAQFRQLPLVVGAGAPQAATSLLDALAKLPALQQRVVAAVRVGQRRWNLLMK
                     SGMNIMLPEGHARIALDRLMELQRDHVVLDRPLAAIDMRLPDRVVFRPQANLNSDTAA
                     NPNAPKKPT"
     gene            complement(2730..>2952)
                     /locus_tag="VFN42_04045"
     CDS             complement(2730..>2952)
                     /locus_tag="VFN42_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995815.1"
                     /translation="AGEGRPSTIFLARMLQSRGWQACARHDGVRCILTSQARSGDWST
                     LFFAGHDDETMAFARHDGGARVHDERPRA"
BASE COUNT          454 a          995 c         1009 g          494 t
ORIGIN      
        1 tgcgttcaag atggccgacc aggtgctgta catgggcgtg cgcggcgcac gccttcgaag
       61 ccgaatggct tggtcaccac gcccacggtc aggatgtttc gttcgcgggc catgcgcgcg
      121 atgaccggcg cggcgccggt gccggtgccg ccgcccatgc cggcggtgat gaacaccata
      181 tgcgcgccgt cgaggtgacg gtacagctcg tcggaagctt cttccgctgc ggccttgccg
      241 atttccggct tcgcgccggc gcccagaccc tgggtgatgt gcggcccgag ctggatgcgg
      301 cgatccgccc ggctgtgcac caactgctgt gcgtcggtgt tcgcgacgac gaactccacg
      361 ccctgcaagt tggccgcgat catgttatcc acagcgttgg tgccgccccc gcccacaccg
      421 atcacggtga tgcggggcga gaaatcggta tgcacctgct gtggaattgt caggttcaga
      481 gtcatgggag cgcctccccg ctgcgagtcg gtgggttagg gtagtgcata cgccaatgcg
      541 agtgattcgc tacccgcatc agacgtggtc cttcaaaaaa ttcaccaggc ggcgaagcag
      601 cccggccggc cgctcttcct ccaggtccat gtcgtgcatg gtgcggcctt cgccggacgc
      661 ccaatgcagc aagccggttg cggtggcgaa ggcggggccc gacgccgaat cgggcaggcc
      721 gcgcacggtg tgcggtttgc cgagccgcac ttggcggttc agcatgcgtc cggccatttc
      781 gcgcaatccg gccagttggc aggcgccgcc ggtcagcacc acgcggttgc cggcggcacg
      841 actcaggccg gaacgatcca gccggtcctt caccatctcg aaggtttctt ccagccgtgg
      901 gcggatgatg ttcaccacca tgctgcgcgg caccttggcg atctggtgtt cttcttcccc
      961 aaccagcggc accggcagca gttcccgatc gtcgtcggcg caggcttgca cgttgccgta
     1021 cagcgtcttc agccgctccg cgtgattgat ggaagtggac aggccggtgg cgagatcctt
     1081 ggtcacgtgc aggccgccga tcggcagttg cgcggtgtgc agcaactggc cctcggcgaa
     1141 caccgccatg ccggtggtgc cgccgcccat gtcgatgatg gtgcagccga gttcgcgttc
     1201 gtcgtccacc agcgtggaca ggccggtggc catcggcgcc gacaccagct cgctgatctc
     1261 caggtcgcag cgcgccatgc aggcgccgag cgagcgcagc gcggtggagg cggcgtcgat
     1321 gatgtgcagg cgcgcgccca gcgtggagca gaacaggcca cgcggatcga gcacgtgctc
     1381 ggtctcgtcc accgcgaaac tcagtggcaa agcgtggatg gattcccgcc cctcggtgac
     1441 ggcgcgcgcc ctgccttcgt tcaccacgcg gcggacatcg gcttcaccga ccgcgcgccc
     1501 gccgaccggc cactgcacgt tgaacagccg tgattccggc tggccgcaac tgagattcac
     1561 cacgaccgaa cgcaggcggg tatccgccat gtcctcggcc tggccgacgc aggcgcgcac
     1621 ggccttctcc gcttcgtcga gatcgacgat gctgccgccg cgcacgccgc gtccgcgatg
     1681 ccagccgaag cccagcacgc gcagcgtgcc atcggattcg gtgcggccga tcaggcaggc
     1741 gatcttggtc gtgccgatgt ccagcacgcc gaacggaccc gagcgatgat gccgtggccg
     1801 ctccggctct tccggcggtg cggtcagcgg cggcacgatc cggcgcgaca tgtcgttcat
     1861 gacgcgtctc ctccctcagg tcggcttctt gggtgcattc ggattcgccg cggtatcgct
     1921 gttcaggttg gcttgcgggc gaaacaccac gcggtccggc aggcgcatgt cgatcgccgc
     1981 cagcggacgg tcgagcacca cgtggtcgcg ctgcaattcc atcagccggt cgagcgcgat
     2041 cctggcgtgg ccttcgggca gcatgatgtt catgccgctc ttcatcagca ggttccagcg
     2101 gcgctgtccg acacgcacgg cggccacgac gcgctgctgc aacgcgggaa gttttgccag
     2161 ggcgtcgagc aggctggtgg cggcctgcgg cgcaccggca ccgacgacca gcggcaactg
     2221 gcggaactgc gccacgtctt cattggtgac gacctggccg ttgcggtcga tcagcacgaa
     2281 cttgccgtcg tgctgccaga tcgcgaaagg gcggcgttct tccagataga ccaccaccgt
     2341 gccgggcagg cggcgctcca ccgtcgcatg atcgacccag gacagcttct cgatgcgctt
     2401 gcgcgcctgc tcgaccgaga agccgagcat cgggctgccg gtgttgacgc caagcgcggt
     2461 gcgcagcgcg gattccggcg tgttgaagcg gcccttgacc acgatatggg tcacgcgcag
     2521 gcccgcgaag gcggtggcgt tgcccaggcg ctcccgaagc gtggccagcg cgccgcccgg
     2581 tgaggcggaa tgcaccaggc cggcgatcat cagcagcgcg atcatcgcgg ccgacgccca
     2641 ggcgaccggg cgcaggaatt tgcggatgcg ccgcagcagc agcgtggtgc gcgccggacg
     2701 gtcctgcacg gaattgcgcg tacggcgggt catgcgcgcg gcctttcgtc atggacgcgg
     2761 gcgcccccgt cgtggcgggc gaaggccatt gtttcatcgt catggccggc gaagaagagt
     2821 gtactccaat cacccgatcg cgcctgggaa gtcaggatgc agcgtacccc gtcgtggcgg
     2881 gcgcaggcct gccacccacg actttgcagc atgcgggcaa gaaaaatcgt ggatggccgg
     2941 ccttcgccgg cc
//
LOCUS       DASSZH010000189         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1044279, whole genome shotgun sequence.
ACCESSION   DASSZH010000189 DASSZH010000000
VERSION     DASSZH010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044279"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VFN42_04050"
     CDS             complement(<1..915)
                     /locus_tag="VFN42_04050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HET8995816.1"
                     /translation="MARSPAVFQRGEVKAGLTPSSPAIDPTPRRSYRFAMPNLDRCLN
                     IWDLRDAARRRLPKGVFDFVDRATEDHIAVANNRSAFERIKLRHRALVDVSQRSPATT
                     LFGKPASLPMAIAPTGAAGLCWHQGELELARAAAAANIPLTLSTASMTAMEKIVAAGG
                     PDKGGRLWFQLYVWKQRELSHELIRRAQRNGFEALIVTVDSPVSPNREYNTHNGFALP
                     FKLTPRFAVDVMRHPAWAQSVLLKYYTGGGFPRHENYPEEYQRSILSGAGAQQGMRAD
                     SLAWEDIAVFRDMWPGVLMLKGVQRPDDA"
     gene            944..1237
                     /locus_tag="VFN42_04055"
     CDS             944..1237
                     /locus_tag="VFN42_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HET8995817.1"
                     /translation="MQDAAFEWDDAKAVLNQRDHGIAFGVARAAFNDAFAIERLDRRH
                     ADPEERYVLLGMVDQHLLVVSYTLRGERIRIISARKAEPHERRRYHKENRQTL"
     gene            1257..1550
                     /locus_tag="VFN42_04060"
     CDS             1257..1550
                     /locus_tag="VFN42_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET8995818.1"
                     /translation="MTDAQVHAAAMRDPDARPMTDAEWASAPRVPRVRTLRRALGMTQ
                     EAFSQRFQIPLGTLRDWEQGKSEPDQSARTYLKVIAADPEAVQRALLAKPRAE"
     gene            1588..1929
                     /locus_tag="VFN42_04065"
     CDS             1588..1929
                     /locus_tag="VFN42_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019087090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HET8995819.1"
                     /translation="MTERLAYKVMAADEFAHMQREQVFHGSAVDRADGFIHLSTAAQL
                     TATVDKHFQGQSDLVVVAVDLSPLGDAVRWETSRGGDLFPHVYGALPMSAVTAAGPLV
                     RGADGAVLLPG"
BASE COUNT          348 a          643 c          699 g          362 t
ORIGIN      
        1 cgcgtcgtcc ggccgctgca cgcctttcag catcagcaca cccggccaca tgtcgcggaa
       61 caccgcgata tcctcccagg cgagtgagtc cgcgcgcatg ccttgctgcg cgccggcacc
      121 gctgagaatc gagcgctggt actcttccgg atagttctcg tgccgtggga aaccgccgcc
      181 ggtatagtat ttcagcagca cgctctgtgc ccaggccgga tgccgcatca catcgacggc
      241 gaagcgcggc gtgagtttga acggcagggc gaagccgtta tgcgtgttgt attcgcggtt
      301 gggcgagacc ggcgaatcga cggtgacgat cagcgcctcg aatccattgc gctgcgcccg
      361 gcggatcagt tcgtgcgaca gctcccgctg tttccagaca taaagctgga accacagcct
      421 gccgcctttg tccggcccgc cggccgcgac gatcttctcc atcgcggtca tcgacgcggt
      481 ggaaagcgtg agcgggatat tcgcggcggc ggcagcgcgc gcgagttcca gctcgccctg
      541 gtgccagcac aatccggcgg cgccggtcgg ggcgatcgcc atcggcaggc tggccggctt
      601 gccgaacagt gtggtggccg ggctgcgttg cgacacatcg accagggcgc ggtggcgcag
      661 cttgatgcgt tcgaacgcgg agcggttgtt ggcgacggcg atgtggtctt ccgtggcgcg
      721 gtcgacgaag tcgaacacgc ccttcggcag ccggcggcgc gcggcgtcgc gcaggtccca
      781 gatgttcagg cagcggtcga ggtttggcat ggcgaaacgg taggagcggc gtggggtggg
      841 gtcaattgcg gggctggatg gcgtcaaccc ggctttaacc tcgccgcgct gaaataccgc
      901 cggcgatctg gccattggca gacctactgg cagccattat ataatgcagg atgcagcctt
      961 cgaatgggat gacgcgaaag cagtgctcaa ccagcgtgac cacggcatag cgttcggggt
     1021 ggcccgcgcg gctttcaacg atgcgtttgc catcgagcgg ctcgatcgcc gccatgctga
     1081 tcctgaagaa cgctacgtgc ttctcgggat ggtcgatcag cacctgctgg tcgtctctta
     1141 tacgttgaga ggcgagcgta ttcgcatcat ctccgcccgc aaggctgaac cacatgaacg
     1201 acgcagatac cacaaggaaa accgccaaac gctttgattg ggaacgagcc gatgcgatga
     1261 cggacgcgca ggtacacgcg gcggcaatgc gtgacccgga cgctcggcca atgacggatg
     1321 ccgagtgggc gtctgcgcca cgcgtgccgc gggtaagaac gttgcgccga gcgctgggca
     1381 tgacgcagga ggcgttttcg caacgtttcc agattccgct tggcacgttg cgggactggg
     1441 aacagggcaa gtcggaaccg gaccagtccg cgcggaccta tctcaaggtg atcgccgcgg
     1501 atcctgaagc cgtgcagcgg gccttgctgg ctaaaccccg ggccgagtga acgcggtggc
     1561 gcgttgtccg cacatcgaga ggaacgcatg accgaacggc ttgcctacaa agtcatggcc
     1621 gccgacgaat tcgcccacat gcagcgcgag caggtgtttc acggctccgc cgtggaccgc
     1681 gcggacggct tcatccatct gtccaccgcc gcgcagctta ccgccacggt ggacaagcac
     1741 ttccagggac aaagcgatct cgtcgtcgtc gcggtcgacc tgtcgcctct gggcgatgcg
     1801 gtgcggtggg agacgtcgcg tggcggcgac ctgttcccgc atgtctatgg ggctttgccg
     1861 atgtcggcgg tgacggctgc cggcccgctg gtgcgcggcg cggatggtgc ggtgctgctg
     1921 cccggctgaa cggcgtgcct ggcctgatcg ccgcgcctgc gcaatccaag cacatccgtt
     1981 tgcgtggcag ggtcgctgat cccgcgccaa gcgcgcgtgg cctgtcatac caacccgcga
     2041 tgcgctcgac tc
//
LOCUS       DASSZH010000190         3301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_720465, whole genome shotgun sequence.
ACCESSION   DASSZH010000190 DASSZH010000000
VERSION     DASSZH010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720465"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..1246)
                     /locus_tag="VFN42_04070"
     CDS             complement(209..1246)
                     /locus_tag="VFN42_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET8995820.1"
                     /translation="MLFRQLFDATSCTYTYLLASRRGGEALIIDPVLERVDRYLQLIR
                     ELDLALVKAVDTHLHADHITGLGALRDRTRCITVMGEQSGVDVVSMRLSDGDTLRIEG
                     LALDVIYTPGHTDDSYSFAMADRVFTGDTLLIRGSGRTDFQNGDPRAQYESIFHRLLR
                     LPEETLVFPAHDYKGDTVSTIGEEKRHNPRLQVASADDYVALMNNLNLSNPKMMDVAI
                     PANMRQGLHQEEVARRGWAVSAEQACALLGRPGVAVIDLRERGERERHGTIPGSLHAP
                     YPDLQENIQPGGILHELATATGKRLVFYCAFGERSAMAVQAAQDAGLAAACHIEGGIA
                     AWKKAGGSIVH"
     gene            complement(1448..1717)
                     /locus_tag="VFN42_04075"
     CDS             complement(1448..1717)
                     /locus_tag="VFN42_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HET8995821.1"
                     /translation="MNTQHSKSTSRSIATGVLLAGLVLGLSGCGYSRGDRAVSGGLIG
                     AGGGAAIGALTGNPLAGAAIGGAGGALVGAATNPHDVNLGRPAWR"
     gene            complement(1765..1977)
                     /locus_tag="VFN42_04080"
     CDS             complement(1765..1977)
                     /locus_tag="VFN42_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995822.1"
                     /translation="MKRKVACGLAVAMMLGLAGCGYSPGTRAVTGGLLGAGAGAGISA
                     ATGGRPATGAAIGAGAGALGGALTAH"
     gene            2154..3197
                     /locus_tag="VFN42_04085"
     CDS             2154..3197
                     /locus_tag="VFN42_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006558701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET8995823.1"
                     /translation="MFSRMLGFMSADMAIDLGTANTLVYVKGRGIVLAEPSVVAIADV
                     RGKKQVVAVGEEAKQMLGRTPGYITASRPLRDGVIADFEVAEEMIKHFIRKVHNRRGF
                     ASPLIIVCVPSGSTAVERRAIQESAESAGARRVQLIEEPMAAAIGAGLPVTEPSGSMI
                     VDIGGGTTEVAVISLGGIVYSRSVRVGGDKMDEAIISYIRRNHNLLIGESTAERIKMD
                     IGAANAPEEGDDGPYREVRGRDLMNGVPREVLVGQRQVAESLSEPVSAIVEAVKVALE
                     NTPPELAADIVDKGIVLTGGGALLYRLDQVLRDATGLAVVVAENPLQCVALGTGRALE
                     ERKRLSNVLTSMY"
BASE COUNT          525 a         1105 c         1129 g          542 t
ORIGIN      
        1 aactcgcgaa atgcgccaac tcagccaggt ttgcacgcca gccgtcgacc ggcacgatct
       61 gcgcgccgcc gaaggcgtcc tggccgcgat cgtgctttcc gccctgccat ggagcgtggc
      121 cctgctgctg ctgcgctgat atcagtgccg ctctcaaccc gagcctcgtg tgataccggc
      181 ggctttgcgt gaaagcgcgg gccgaggctc aatgcacgat ggagccgccc gccttcttcc
      241 atgcggcgat gccgccttcg atgtggcagg cggcggccag ccccgcatcc tgcgccgcct
      301 gcaccgccat cgcggaccgc tcgccgaatg cgcaatagaa caccagccgt ttcccggtgg
      361 cggtcgccag ctcgtgcagg atgccaccgg gctggatgtt ctcctgcagg tccggatagg
      421 gcgcatgcaa cgagcccggg atcgtgccgt gccgctcccg ctcgccgcgt tcgcgcagat
      481 caatcaccgc cacgccgggc cggcccagca gcgcgcaagc ctgctcggcg ctgaccgccc
      541 agccacgccg ggcaacttct tcctgatgca gtccctggcg catgttggcg gggatcgcga
      601 cgtccatcat ctttggattg gacaggttca ggttgttcat cagcgcgacg taatcgtccg
      661 ccgacgcgac ctgaagccgc ggattgtggc gcttctcctc gccgatggtg ctgacggtgt
      721 cgcccttgta gtcatgggcc gggaagacca gcgtctcctc cggcagccgc agcaggcgat
      781 ggaagatcga ctcgtactgc gcacgcgggt cgccgttctg gaaatcggtg cggccggagc
      841 cgcggatcag caaggtgtcc ccggtgaaca cgcggtcggc catggcgaag ctgtaggagt
      901 cgtcggtgtg gccgggcgtg tagataacgt ccagcgccag gccctcgata cgcagtgtgt
      961 cgccgtcgga caggcgcatg gagacgacat cgacgccgga ctgctcgccc atcacggtga
     1021 tgcaccgcgt gcggtcgcgc agcgcgccga ggccggtgat gtggtcggcg tgcaggtggg
     1081 tgtccaccgc cttgaccagc gccaggtcga gttcccggat cagctgaagg tagcggtcga
     1141 cccgctccag caccggatcg atgatcagcg cttcgccacc gcgccgggat gccagcaggt
     1201 aggtgtaggt gcaggatgtc gcgtcgaaca gctggcggaa cagcatcggc ccctccctgg
     1261 acccggccgc tgagagcgat cgtctcgcgc ggcgccgcag gataggacaa gctgtcccgg
     1321 cattgccagc attggaaaaa ttgcgctccc gcggatgccg cgcggggcaa tctggcggcg
     1381 cggcatcgct tccgcgccag gggaagcagg gaccagaaga gccttgcggc tctcctggtc
     1441 tcgccggtca gcgccaggcc ggacggccga ggttcacgtc gtgtgggttg gtggcggccc
     1501 caaccagcgc accgccggcg ccgccgatcg cagcgccggc cagcggattg ccggtcagtg
     1561 ccccgatggc ggccccgccg ccggcgccga tcaggccacc cgacaccgcc cggtcaccgc
     1621 ggctgtagcc acagccggaa agcccgagca ccagcccggc cagcagcacg cccgttgcaa
     1681 tgctgcggct cgtcgatttg ctgtgttgcg tgttcatgtt gattgcgatt gcatcctttc
     1741 ggggtcaggg aaaaactact tggttcagtg tgcggtcagc gcgccgccca gggcgcccgc
     1801 tccggcgccg atcgccgcgc cggttgccgg ccgtccgccg gtcgccgccg aaattcccgc
     1861 gccggccccc gcgcccagca ggccgccggt caccgcgcgc gtcccggggc tgtagccgca
     1921 gccggccagg cccagcatca tcgccacggc cagtccgcag gcgactttac gtttcatagg
     1981 ctctcctccg attgctagcg cttcgcgaac gatttccacc cctggcgcga ggaattccgg
     2041 tggccggaat gaggcaaagc cgaggccgcc gatcaccccc ttgttaacgc cccgcgcggg
     2101 ggctaagccc accgcctccc tcgctgtttc cagcttctgc aaaggctttc tgcatgtttt
     2161 ctcgcatgct tggtttcatg tcggcagaca tggccatcga tctcggcacg gccaacactc
     2221 tggtttacgt caagggccgc ggcatcgtgc tggcggagcc aagcgtggtt gccatcgccg
     2281 atgttcgcgg caagaagcag gtggttgccg tgggggaaga ggccaagcag atgcttgggc
     2341 gcactcccgg ctatattacc gccagccggc cgttgcgcga tggtgtgatc gccgacttcg
     2401 aagttgcgga ggaaatgatc aagcatttca ttcgcaaggt acataatcgc cgaggtttcg
     2461 cctcaccgtt gatcattgtc tgcgtgccgt cgggatccac tgcggtggaa cgccgcgcca
     2521 tccaggaaag cgccgaaagc gccggagcgc ggcgggtgca gctgatcgag gagccgatgg
     2581 ccgccgcgat cggcgccggg ctgccggtca ccgagccgtc cggatccatg atcgtggata
     2641 tcggcggcgg caccaccgaa gtggcggtga tctcgctggg cggcatcgtc tattcgcgca
     2701 gcgtccgggt cggcggcgac aaaatggacg aggccatcat ttcctacatc cgccgcaacc
     2761 ataacctgct gatcggcgag agcacggcag agcggatcaa gatggacatc ggcgcggcga
     2821 acgcaccgga ggaaggtgac gacggcccgt accgggaggt gcgcggccgc gacctgatga
     2881 acggcgtgcc gcgcgaagtg ctggtgggcc agcgccaggt ggcggagagc ctcagcgagc
     2941 cggtctccgc catcgtcgag gcggtgaagg tggcgctgga gaacaccccg cccgagctgg
     3001 cggcggacat cgtggacaag ggcatcgtgc tgaccggcgg tggggcgctg ctctaccggc
     3061 tggaccaggt gctgcgcgac gcgaccgggc tggcggtggt ggtggcggag aacccgctgc
     3121 aatgcgtcgc cctgggcacc ggccgcgcgc tggaggaacg caagcgcctg tcgaacgtgc
     3181 tgaccagcat gtactgagcg ttctccgcgc ttccgatcgc acgattgcct tgcggctggc
     3241 ccgacgcgct tgtatctgca aagccatagc tgcgagaggg caacgacatg gcacgggccg
     3301 g
//
LOCUS       DASSZH010000191         2476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1112497, whole genome shotgun sequence.
ACCESSION   DASSZH010000191 DASSZH010000000
VERSION     DASSZH010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112497"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /gene="hisD"
                     /locus_tag="VFN42_04090"
     CDS             <1..822
                     /gene="hisD"
                     /locus_tag="VFN42_04090"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HET8995824.1"
                     /translation="YRGAAAAAIVAAQHLAGADEIYCLGGVQAVAAMALGTAAIAPVD
                     MLVGPGNAYVAEAKRQLFGKVGIDLLAGPTETLIIADDTADAEMCATDLLGQAEHGPT
                     SPAILLTTSTKIANEIQPEIERQLQRLPTAAIAGVAWRDHGQVILCDSDEEMLSEADR
                     IASEHVQVLTRNPRWFLDRMTNYGSLFLGKETNVAYGDKVIGTNHTLPTKRAARYTGG
                     LWVGKFLKTVTYQEVSPEATAMIGAYCSRLCAIENFAAHKEQADLRVRRYGGRNA"
     gene            complement(859..>2476)
                     /locus_tag="VFN42_04095"
     CDS             complement(859..>2476)
                     /locus_tag="VFN42_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995825.1"
                     /translation="RWLRQTCGVDSVTLVGARIGAAIAARAACAIDGIEALIMVSPVA
                     SGRRFLRELVLAAHTNAEIWQVEPRIDDGMWFEAHGLRLDRVTRNALQRLDVANLPSC
                     PAPNVLVLDSPDSASARSLVRRLGSTGAQVTQVSDPVLAAMLRDSHENEVPHEAFAQA
                     VAWHAALNPSDAQCHGGVTLPAAQSAVLALDTSTERPLWFGPDHALLGVLCEPTRAAP
                     DAPVVLIANTGANPRYGNSRAAVTVARWLATLGVPSLRIDGTGIGDSLPQTGERGLPY
                     SLQGDADLQAGIDALTARFSGPVIVLGMCSGAYHALRGAFQDTRIRGLMLLNLQKFVW
                     RDGESLSVVQRTTLRTTRFYMRNAISGATLRRLLRGDINVAGISKGLAGRAGRRVAAA
                     CDPAIRMLQRKETPVGRVRRQVRELAARSVQILFILSGNDPGLDEIAEYFGAQGRMFR
                     RLPNVTYQTLEGADHTLSANWARQALMQRIAVYLNRYFDVPIPMERAEVAAPRAVCRQ
                     PDPSLESDCFVQPGVFAQSVAIATGASHAG"
BASE COUNT          434 a          879 c          797 g          366 t
ORIGIN      
        1 taccgggggg cggcggcggc cgcgatcgtc gcggcccagc acctggccgg cgccgacgag
       61 atctattgcc tcggcggcgt ccaggcggtc gccgcgatgg ccctgggcac ggcggcgatc
      121 gccccggtcg acatgctcgt cggacccggc aacgcctacg tggccgaggc caagcgccag
      181 ctcttcggca aggtcggcat cgacctgctc gccggcccga ccgagaccct gatcatcgca
      241 gatgacaccg ccgatgccga aatgtgcgcc accgatctgc tcggccaggc cgaacatgga
      301 ccgacgtctc ccgcaatcct gctgaccacg tcaacgaaga tcgcgaatga gattcagccg
      361 gagatcgagc gccagctgca gcgcctgccc accgccgcca tcgccggcgt ggcatggcgc
      421 gaccacggcc aggtgatcct gtgcgactcg gatgaggaga tgctgtccga agccgaccgc
      481 atcgcatcgg agcatgtgca ggtgctgacc cgcaacccgc gctggttcct ggaccgcatg
      541 accaattacg gctccctgtt cctgggcaag gaaaccaacg tcgcttacgg cgacaaggtg
      601 atcggtacca accacacgct gcccaccaag cgcgctgcgc gctacaccgg tgggctgtgg
      661 gtgggcaagt tcctgaagac ggtcacctac caggaggtca gtcccgaggc gaccgcgatg
      721 atcggtgcgt actgctcacg cctctgcgcg atcgaaaact ttgccgcaca caaggaacaa
      781 gccgatctgc gtgtgcgccg ctacggcggc cggaacgcct gagcgacgag cacagccagg
      841 ccggctcgcc ccgtttcgtc agccggcatg cgacgccccg gtggcaattg ccacggactg
      901 cgcgaagacg cccggctgca cgaaacaatc ggattccagc gatggatcgg gttgccggca
      961 gacggcacgc ggcgccgcaa cttcggcacg ctccataggg atcggcacgt cgaaatagcg
     1021 gttcagatac acggcaatcc gctgcatcag tgcctggcgt gcccagttgg cgctcagcgt
     1081 gtgatccgcg ccctccaacg tttggtaggt gacgttcggc aggcggcgga acattcgacc
     1141 ctgcgccccg aaatattcgg cgatctcgtc cagaccagga tcattgccgc tgaggatgaa
     1201 caggatctgc accgaccgcg ccgccaactc gcgcacctgc cgccggaccc gccccaccgg
     1261 cgtttctttg cgctgcagca tgcggatggc cggatcgcac gccgctgcca cgcgccgccc
     1321 ggcccgccct gccagtcctt tgctgatacc ggcaacgttg atgtcgcccc gcagcaagcg
     1381 gcgcagcgtc gcgccgctga tcgcattccg catgtagaag cgtgtcgtcc gtaaggtggt
     1441 tcgctgcacc acggacagcg attctccgtc ccgccagacg aatttctgca gattcagcag
     1501 catcaggccg cggatacgcg tgtcctggaa cgcgccgcgc aacgcgtggt acgccccgga
     1561 gcacatgccc agcacgatca ccggcccgct gaaacgcgcg gtcagcgcat cgatccccgc
     1621 ctgcagatcc gcgtcaccct gcagcgaata cggcagtccg cgttcgccgg tctgcggcag
     1681 cgagtcgccg atcccggtgc cgtcaatgcg cagcgaggga acgccaagcg tcgccaacca
     1741 gcgtgcgacc gtgacggctg cccgggaatt tccgtaccgc ggattcgccc cggtgttggc
     1801 gatcagcacg acaggtgcat ccggcgccgc gcgggtcggc tcgcacaaca cgcccagcag
     1861 cgcgtgatcc ggaccgaacc agagcggccg ctcggtggac gtgtccagcg ccagcacagc
     1921 cgattgcgcc gccggcagag tcacaccgcc gtgacactgc gcatcggatg ggttcagggc
     1981 ggcatgccag gccacggcct gcgcgaacgc ctcgtgcggc acctcattct cgtgagagtc
     2041 gcgcagcatc gccgccagga ccgggtcact gacctgggtc acctgtgcac ccgtgctgcc
     2101 cagtcgccgc accagggaac gcgccgaggc gctgtccggg gagtcgagta ccagcacgtt
     2161 cggtgcgggg caggatggaa ggttggcgac gtccagccgt tgcagcgcat tccgtgtcac
     2221 gcgatccaga cgcagcccgt gcgcctcgaa ccacatgccg tcgtcaatgc gcggttcaac
     2281 ctgccagatc tcggcattgg tgtgcgcggc gagcaccagc tcgcgcagga accgccggcc
     2341 gctggcgacc ggagagacca tgatcagcgc ttcgatccca tcgatggcgc aggctgcccg
     2401 tgcggcgatc gcagccccaa tgcgtgcccc gaccagggtg accgagtcga cgccgcaggt
     2461 ttggcgcagc catcgc
//
LOCUS       DASSZH010000192         2418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_635215, whole genome shotgun sequence.
ACCESSION   DASSZH010000192 DASSZH010000000
VERSION     DASSZH010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635215"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VFN42_04100"
     CDS             complement(<1..314)
                     /locus_tag="VFN42_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET8995826.1"
                     /translation="MPFYERGDVRIRYEEAGSGFPLLVTPGGGLNSRVANWPSAVFNA
                     PELFANEFRCITMDQRNANGGESTGPVPVDNPWDAFADDQLGLMDHLGIREFFYMGYC
                     IGG"
     gene            624..1058
                     /locus_tag="VFN42_04105"
     CDS             624..1058
                     /locus_tag="VFN42_04105"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HET8995827.1"
                     /translation="MPKILIIHGAGMNMRGKVQTEIFGPMTLPQYDEHIRGYAAELGV
                     DIEIFHSNVEGEVVNKIYEASERGFDAAIFNPAAFGRGYPALNAAISQVKFPTIEVHI
                     SNPVRRGPASETLAVSLGSVTGFGVAGYYLALRGLRDMVAKK"
     gene            1068..1580
                     /locus_tag="VFN42_04110"
     CDS             1068..1580
                     /locus_tag="VFN42_04110"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET8995828.1"
                     /translation="MAASLAELRAQRLELENRIETRQRELGPEHPELADDLRDLGRLA
                     HELNDHMDAMALLRRACALHTAAHGPEHPDVATDLNHLGLVYHDLGELTEAQLVLERA
                     LAIDEQALGSEHRNVARDANNLAGVLRDQGDLYSARAALQWSLRVYNAQLGPDHSTTR
                     SVARNLALLG"
     gene            1647..2357
                     /gene="hisG"
                     /locus_tag="VFN42_04115"
     CDS             1647..2357
                     /gene="hisG"
                     /locus_tag="VFN42_04115"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003622236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HET8995829.1"
                     /translation="MTAPFPTAALAPALDSEAETDSPLILALPKGRILAEAGQVLARA
                     GVNPAADYADEDSRRLRFPTDDPMLDVVRVRPFDVATFVAFGAAHIGICGADVLMEFD
                     YPEVYAPLDLGIGKCRVSVAEPVATAGSDDPTRWSRVRVASKYPNIARRHFANRGVHA
                     EVVHLNGAMELAPGLGLARLIVDLVQTGSTLKANGLVETEVIANVTSRLIVNRTALKT
                     RPELIGGWIARFRKALGQ"
BASE COUNT          406 a          820 c          819 g          373 t
ORIGIN      
        1 ccgccgatgc agtagcccat gtagaagaac tcgcgaatgc ccagatggtc catcagcccc
       61 agctggtcgt cggcgaacgc gtcccacggg ttgtccaccg gcaccgggcc ggtcgactcg
      121 ccgccattgg cgttgcgctg atccatggtg atgcagcgga actcgttggc gaacagctcc
      181 ggcgcattga acaccgcgct cggccagttg gcgacgcggg agttcaggcc gccgcccggg
      241 gtcaccagca gtggaaaccc cgagcccgct tcctcgtacc ggatgcggac atcgcctctc
      301 tcatagaacg gcatgacggc tcctcctgag tgtttgctcc cgagtggcgc gtgccaggca
      361 ttggcagcaa tccgcgtgac ggggtgtgct tattcggtgc gggagttcag cccaattcgg
      421 gcggcggctc aaccgcgccc gcgtcgccag cgttccggcc cgcgccagga ccgatcgcgc
      481 tgtgcaacaa gcggcgccgt cgggttgctg aaccggggtc gcgcatggat actccgccca
      541 ccctgaacct gggggaaggg gcgtacacgc gcttccggac cggctgtttc cggtcacgcc
      601 atacagagcg aggcagtcca cgaatgccaa aaatcctgat catccacggc gccggcatga
      661 acatgcgcgg caaggtgcag acggaaatct tcggcccgat gacgctgccg caatacgacg
      721 agcacatccg cggctacgcc gccgaactcg gcgtggacat cgagatcttc cactccaacg
      781 tggaaggcga ggtggtcaac aagatctacg aggccagcga acgcggcttc gacgcggcga
      841 tcttcaaccc ggccgccttc ggccgcggct acccggcgct gaacgccgcg atcagccagg
      901 tgaagttccc caccatcgag gtgcacatct ccaacccggt gcgccgcggc ccggcgtcgg
      961 agacgctggc ggtcagcctg ggctcggtca ccggcttcgg tgtcgccggc tactacctgg
     1021 ctctgcgggg gctgcgcgac atggtggcga agaagtaggc ggggcgcatg gctgcctccc
     1081 tcgccgagct gcgcgcgcaa cgcctggagc tggaaaaccg gatcgaaacg cggcagcgcg
     1141 aactgggacc ggaacatccg gagctggcgg acgatctccg cgacctcggc cgcctggcgc
     1201 acgagctgaa cgaccacatg gatgcgatgg cgctgctgcg ccgcgcctgc gctctgcata
     1261 ccgcggcgca cgggccggag catccggacg tggcgaccga cctgaaccat ctcggcctgg
     1321 tttatcacga cctgggcgaa ttgactgagg cccagcttgt gctggaacgc gccctggcga
     1381 tcgacgagca ggcgctcggc tcggagcacc gcaacgtggc acgcgatgcc aacaacctgg
     1441 ccggcgtgct gcgcgatcaa ggggaccttt attcggcccg ggcagcgctg caatggtcat
     1501 tgcgggtgta caatgcccag ttggggccgg atcacagcac gacacgatcc gtcgcgcgca
     1561 atcttgcgct gctgggatga accgggcggg gttccagccg ctccgctccg ggtgtaaacg
     1621 cccgcaactt cgcgaccgga gaccgcatga ccgcgccctt cccgaccgcc gcgctggcgc
     1681 ccgcgctgga cagcgaggcc gagactgact cgccgctgat cctggccctg ccgaaagggc
     1741 gcatcctggc cgaggccggc caggtcttgg cacgcgccgg cgtcaacccg gcggccgact
     1801 acgccgacga ggacagccgg cggctccgct tccccaccga cgatcccatg ctggacgtgg
     1861 tacgggtgcg gcccttcgac gtcgccacct tcgtcgcctt cggcgcagcg catatcggca
     1921 tctgcggcgc cgacgtgctg atggagttcg attatcccga ggtttacgca ccgttggacc
     1981 tgggcatcgg caaatgccgc gtctcggtcg cggagccggt ggcgacggcg ggcagcgacg
     2041 atcccacccg ctggtcgcgg gtgcgggtcg cgtcgaaata ccccaatatt gcccgccgcc
     2101 acttcgccaa tcgcggcgtg cacgccgaag tggtgcatct gaacggcgcc atggaactgg
     2161 caccgggcct gggtctggct cggctgatcg tggatctggt gcagactggt tccacgctga
     2221 aagcgaacgg gctggtggag acggaagtga tcgccaatgt caccagccgt ctgatcgtca
     2281 accgcacggc gctgaaaacc cgcccggagc tgatcggcgg ctggatcgcc cgcttccgca
     2341 aggcgctggg gcagtgatgc ggggataccc agaatacgcc ccctcccctt gcgggagggg
     2401 gttgggggga gggggcgc
//
LOCUS       DASSZH010000193         4420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_481726, whole genome shotgun sequence.
ACCESSION   DASSZH010000193 DASSZH010000000
VERSION     DASSZH010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481726"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..1119)
                     /locus_tag="VFN42_04120"
     CDS             complement(199..1119)
                     /locus_tag="VFN42_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017957005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET8995830.1"
                     /translation="MTDAPKPVEAVLLPRTHDVGGFEVRRALPAQERRMVGPFVFFDQ
                     MGPGEFLVGRGLDVRPHPHIGLSTVTYLFEGEILHRDSLGSRQPIVPGDVNWMTAGAG
                     IAHSERTDPVQRVHSNRLFGIQSWVALPKSAEESAPGFVHHPAATLPLVEHDGVRMRL
                     IAGTGWVLAAPVTVSSPLFYADAELAPNAAVPLPEEHEERAAYVVRGEVAVAGDRFEA
                     GRMLVFRAGDRLALRAGPQGARLLLLGGAAMDGPRYLFWNFVSSSRERIEQAKADWKA
                     GRFGKVPGDETEFIPLPEERVAVSTGSATS"
     gene            complement(1184..2470)
                     /locus_tag="VFN42_04125"
     CDS             complement(1184..2470)
                     /locus_tag="VFN42_04125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8995831.1"
                     /translation="MKFLFVHQNFPGQFLHLARHLAASKQHEVVFITEPNANQIGGVR
                     KVPYNKPLPASAATHVAVRELDIAVRRAEVVAAVARNLKSLGFVPDIMIGHHGWGEMM
                     NLVDVFPGVPLLGYQEFYYQIDGIDVNFDPEFPTPVIDYPRIRAKNAINHITLNLGQH
                     GQTPTEWQLSTYPDWARPQITLLREGVHLDVCKPDPAVRTAPLKVGDMTIRPKDKLVT
                     YVSRDLEPYRGFHVMMRALPHLLRARKDLRVVMIGGDGVSYGNAPAEGAWRQVMLKEV
                     GDRIDMKRVVFPGRLDYQDYLAVLQRSDAHVYLTYPFVASWSLRESLATGCVVIGSDT
                     QPVREFITPGVNGLLTSFFDPAALARTVLNVLEDADLSRRLRENARRYAERHLAMADY
                     LAEYEALISRLTGMQLAAPQEETVVPLRRPARRARR"
     gene            complement(2495..3448)
                     /locus_tag="VFN42_04130"
     CDS             complement(2495..3448)
                     /locus_tag="VFN42_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015073197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995832.1"
                     /translation="MPEAAHAVAPQAAPQGANPAAQEGAQNRVAELRVSGHLMTLDTG
                     VFCVFAAPGSPAPDRATGLPGVRLSLPPGPAGRPEAVTISTFRSDGWVDGAALVRVSQ
                     GPAQVMVTIYQAPQASEGAPRLQVTKLTGESPGTAAPPAPAAVAARTGEPEVIAHVQR
                     AGDVGARMGDWIGTRGSRAWIEGFGIAPQEGIGPDDIEYQAVLGRGWLSPWVEGGKFC
                     GSRGMALPLLGMTIRLKGAAARAWELSYSATFTDGSEVGPVAGGETCEAESLASLEAF
                     QVVLRRRSGRAPEKPAEKPSAAPRTAGRATRPIARAKSGTR"
     gene            complement(3498..>4420)
                     /locus_tag="VFN42_04135"
     CDS             complement(3498..>4420)
                     /locus_tag="VFN42_04135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8995833.1"
                     /translation="GDFPNLRYRLPEDLPALLDLLRAERPKRAEVHHLFGHPPEIHDL
                     LARLRLPYAVHVHDYAWFCPRISLVGGERRYCGEPALARCEACVADHGALNEETIGVA
                     ALRDRSARLLSAAEQVVTPSHDTATRMRRHFPELRPVVVPHENDAAVPPPPAPSARDG
                     RCRVCVLGAIGLHKGYDVLLACARDAAERRLPLDFVVVGHTTDDARLLATGRVFVTGP
                     YRSEEAVPLLHAQRGSIGFIPSICPETWSLALTELWRAGLHVAAFDFGAPAERIRRTG
                     RGLILPVGLPPHAINNALVAASGLTVHEGT"
BASE COUNT          683 a         1545 c         1538 g          654 t
ORIGIN      
        1 cgcaagacca agcgtgttcc gttgcaaccc gacccgatcg ttccggtgta gatggccggg
       61 tcgagcccgg ccacgacgag ggggaaggcg ttctttccag cccgacgtaa gcgcttctgt
      121 cacacccgga ccttcgcccg ccaggacgcc gcacgatgcg gcatgcgcct gcgcgccggc
      181 tcggcccgcg aaccggtatc agctcgtcgc cgacccggtg ctgaccgcga cgcgctcctc
      241 cggcagcggg atgaactcgg tttcgtcgcc gggcaccttg ccgaagcggc cggccttcca
      301 gtccgccttg gcctgctcga tccgctcacg cgaggagctg acgaagttcc agaacaggta
      361 gcgcggacca tccattgccg cgccgcccag cagcagcagg cgggcgccct gcggaccggc
      421 acgcagcgcc agccggtcgc cggcgcggaa caccagcatg cggcccgctt cgaagcggtc
      481 gcccgccacc gccacctcgc cgcgcacgac ataggccgcg cgttcctcgt gctcctccgg
      541 cagcggcacg gcggcattcg gggccagttc cgcatccgcg tagaacagcg gcgaggatac
      601 cgtgaccggc gcggccagta cccagccggt tccggcgatc agccgcatgc ggacgccgtc
      661 gtgctcgacc agcggcaggg tggcggccgg atggtgcacg aagccggggg cggattcctc
      721 ggcggatttc ggcagcgcga cccaggactg gatgccgaac aggcggttgc tgtgcacccg
      781 ctgcaccggg tcggtgcgct cggagtgcgc aatgccggca ccggcggtca tccagttcac
      841 gtcgcccggg acgatcggct ggcgggagcc caggctgtcc cggtgcagga tctcaccctc
      901 gaacaggtag gtgacggtgg acaggccgat atgcgggtgc ggccggacgt ccaatccgcg
      961 tccgaccagg aactccccgg ggcccatctg gtcgaagaag acgaaggggc cgaccatgcg
     1021 gcgttcctgc gccggcaggg cgcggcgcac ctcgaacccg cccacgtcgt gcgtgcgcgg
     1081 cagcagcacg gcttccaccg gtttcggcgc gtcggtcatg cccagctccc catgatggcg
     1141 cggccacata tggccgcgtt tcggcgcggc gccactcccg gcgctatcgc cgggcgcggc
     1201 gggccggacg tcgcagaggc accacggttt cttcctgcgg cgcggccagt tgcatgccgg
     1261 tcagccggct gatcagcgcc tcatactccg ccaggtaatc cgccatcgcc agatgccgtt
     1321 ccgcgtagcg gcgggcgttc tcccgcaggc ggcgcgacag gtcggcatcc tccagcacgt
     1381 tcagcaccgt gcgggccagc gcggcgggat caaagaatga tgtcagcagc ccgttcacgc
     1441 ccggggtgat gaattcccgc accggctggg tatcgctgcc gatcaccacg catccggtgg
     1501 ccagcgattc gcgcagcgac catgaggcga cgaaggggta ggtcagatag acatgcgcgt
     1561 cggagcgctg cagcaccgcg aggtaatcct ggtaatccag ccggccggga aacacgacgc
     1621 gcttcatgtc gatgcggtcg ccgacttcct tcagcatcac ctgccgccac gcgccttcgg
     1681 ccggagcgtt accgtagctg acgccgtctc cgccgatcat caccacgcgc aggtccttgc
     1741 gcgcgcgcag caggtgcggc aacgcccgca tcatgacatg gaagccgcga tacggctcca
     1801 ggtcgcgcga cacataggtt accagcttgt ccttcggccg gatcgtcatg tcgccgacct
     1861 tcagcggcgc ggtgcgcacc gcgggatccg gcttgcacac gtccagatgc acgccttccc
     1921 gcagcagcgt gatctgcggc cgcgcccaat cgggataggt ggacaattgc cactcggtcg
     1981 gggtctggcc gtgctggccc aggttcagcg tgatgtggtt gatcgcgttc ttggcgcgga
     2041 tgcgcggata gtcgatgacc ggggtgggga attccgggtc gaagttaacg tcgatcccgt
     2101 cgatttgata atagaactcc tggtagccga gcaggggcac gccggggaac acgtcgacga
     2161 ggttcatcat ctcgccccag ccgtgatggc cgatcatgat gtccgggacg aagccgagcg
     2221 atttcaggtt ccgcgcgacc gccgcgacca cctcggcgcg gcgcacggcg atgtcgagct
     2281 cccgcacggc gacatgcgtg gccgccgatg cgggcagcgg cttgttgtac ggcaccttgc
     2341 gcacgccacc gatctggttg gcgttcggct cggtgatgaa caccacctcg tgctgcttcg
     2401 acgcggcaag gtgccgggcg agatgcagga actggccagg aaagttctgg tggacaaaca
     2461 ggaacttcac gctgcgatcg cctgtccgcc gcggtcagcg cgtacctgat ttggcgcgcg
     2521 cgatcggccg tgtggcgcgg cccgcggtgc gcggcgcggc actgggcttc tccgccggct
     2581 tttccggcgc ccggccggag cggcggcgca gcaccacctg gaacgcttcc aacgacgcca
     2641 ggctctccgc ctcacaggtt tcgccaccgg ccaccggacc gacttcgctg ccatcggtga
     2701 aggtggcgga atagctgagt tcccaggccc gcgccgcagc gcctttcagg cggatggtca
     2761 tgcccagcag cggcagtgcc atgccgcggc tgccgcagaa cttgccacct tccacccagg
     2821 gcgacagcca gccgcgcccg agcactgcct gatactcgat gtcgtccggt ccaatgccct
     2881 cctgcggtgc gataccgaaa ccttcgatcc aggcgcggct gccgcgcgtg ccgatccagt
     2941 cacccatgcg ggcgccgaca tccccggcgc gctgcacatg cgcgatcact tcgggctcgc
     3001 cggtgcgggc agcgaccgca gcgggggcgg gtggggcggc ggtgccaggc gactcgccgg
     3061 tcagcttggt cacctggagg cggggtgcgc cttcgctggc ctgcggcgcc tggtagatgg
     3121 tgaccatcac ctgcgccggt ccctgcgaca cgcgcaccag cgcggcgccg tccacccagc
     3181 catcggagcg gaaggtgctg atcgtgaccg cttccggccg ccccgccgga cccggaggca
     3241 gcgacaggcg cacgcccggc agccccgtgg cgcgatccgg tgcggggctt ccgggcgcgg
     3301 caaacacgca gaacacaccg gtatccagcg tcatcagatg gccggagacg cgcaactcgg
     3361 cgacacgatt ctgggcgcct tcctgtgcgg cgggattcgc gccttgcggc gcggcttgcg
     3421 gggcaaccgc gtgggcggct tcgggcatgc gtgactctcg ctctctcggg gtgcgtggca
     3481 gccggcccga ctgccagtca ggtcccttca tgaactgtca aaccggatgc tgcaacaagt
     3541 gcgttattga tggcgtgcgg tggcaatccg accggcagga tcaagccccg cccggtgcgg
     3601 cgaatccgct ccgctggcgc accgaaatcg aaggccgcaa cgtgcaagcc ggcgcgccag
     3661 agttcggtca gagccaggct ccaggtctcc gggcagatcg acggaatgaa gccgatgctt
     3721 ccccgctgcg cgtgcagcag cggcacggct tcctccgagc gataggggcc ggtgacgaac
     3781 acgcggccgg tcgccagcag acgggcgtcg tccgtggtgt gtccgaccac gacgaagtcc
     3841 agcggcagcc ggcgctccgc ggcatcccgg gcgcaggcca gcagcacgtc gtagccctta
     3901 tgcaggccga tcgcgcccag cacgcacacg cggcagcgcc cgtcgcgcgc ggacggggcc
     3961 gggggaggcg gcacggccgc atcgttctca tgcggcacca ccacgggacg cagctcggga
     4021 aagtgccggc gcatccgggt ggcggtgtcg tgtgacggcg ttaccacctg ctcggcggct
     4081 gacagcagcc gtgcggaacg gtcgcgcagc gcggccaccc cgatcgtttc ctcattcagc
     4141 gcgccgtggt cggcgacaca ggcttcgcat cgggccagcg cgggttcgcc gcaatagcgc
     4201 cgctcgccgc cgaccaggga gatgcgcggg cagaaccagg cgtagtcgtg cacgtgcacg
     4261 gcatagggca ggcgaagccg cgccagcagg tcgtggattt ccggaggatg cccgaacagg
     4321 tgatgcacct cggcgcgctt tggccgttcg gcgcgcagca ggtccagcag ggcgggcaag
     4381 tcctccggca ggcggtagcg caggttgggg aaatccccgg
//
LOCUS       DASSZH010000194         6230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1538727, whole genome shotgun sequence.
ACCESSION   DASSZH010000194 DASSZH010000000
VERSION     DASSZH010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1538727"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /locus_tag="VFN42_04140"
     CDS             <1..1196
                     /locus_tag="VFN42_04140"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET8995834.1"
                     /translation="GEIIETGMVADPPVADPALQLHMPPFRPTPADDEIERAAAALRS
                     ASRVVIVAGAGATASQAGPELLALGQLLAAPIATSLGARGIIPTQHRLSVGCAGNYAA
                     PPANQIVHEADLVLFVGCHTGDQVTHTWRIPPLSTPCVQIDIDPLELGRTYANTLGLM
                     GDPKATLAKLIAALGKLERDTSFAHRAAGIVAAWREARAPLLAQNAVPIYPDRLCAEI
                     TQALPEDGILVADTGYSGIWSSTLIELNGTGQTYLRAAGSLGWSFPAALGAKCAAPQR
                     KVICWSGDGALYYHLTELETARRRGIAVVLVVNNNSGFGQGWPNIQRQQGNKPGDVAE
                     LVRFGPTNFAEVARSFGLRGIRVEQPARIAPALRDALASDETVVVDVATDIDCRAPEP
                     WLPAG"
     gene            complement(1281..1646)
                     /locus_tag="VFN42_04145"
     CDS             complement(1281..1646)
                     /locus_tag="VFN42_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995835.1"
                     /translation="MTRTVHVHVGETLETAGRRFIEGWQRAEHCELTPANAELHVGFE
                     SWETMVRTLSPKRLGLLRHVHQHPAKNVRDLARALGRDYRRVHEDVTALQAAGLLDRD
                     KDGVRAEYDSFDVRMQVAL"
     gene            complement(1643..1939)
                     /locus_tag="VFN42_04150"
     CDS             complement(1643..1939)
                     /locus_tag="VFN42_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HET8995836.1"
                     /translation="MPKAEKLLDIRLETDDGFLIALWLWRVPQPVRPARHSFKYALFF
                     GRPGKRLVLFDNERGKGDHKHILGVESSYEFSTPEKLIADFEAAVRAMREGDKG"
     gene            2219..4024
                     /gene="lepA"
                     /locus_tag="VFN42_04155"
     CDS             2219..4024
                     /gene="lepA"
                     /locus_tag="VFN42_04155"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HET8995837.1"
                     /translation="MTDTPIDHIRNFAIIAHIDHGKSTLADRLIQATGALSDREMTAQ
                     VLDNMELERERGITIKAQTVRLTYPAKNGQVYELNLMDTPGHVDFAYEVSRSLAACEG
                     SLLVVDASQGVEAQTLANVYQAIDANHEIVPVLNKIDLPAAEPERVKQQIEDVIGLDA
                     SDAVEISAKTGLNIEGVLEALVHRLPPPKGDGNAPLKALLVDSWYDQYLGVVILVRVK
                     DGRLKRAQKIRMMSTGAVHQVEQVGVFSPKMIPTDDLGPGEIGYITAAIKTVADCNVG
                     DTITEDRNPAAEPLPGFKPSIPVVWCGLYPVDADEFEKLRESLGRLRLNDASFHYEPE
                     TSAALGFGFRCGFLGLLHLEIIQERLTREFDLDLIATAPSVVYHIFKTNGEMMELHNP
                     VDMPDGSVIDHIEEPWIRATIMVPDDYLGAVLSLCTERRGVQVDLTYVGNRAMAVYRL
                     PLNEVVFDFYDRLKSVSRGYASFDYQMDGYAEGELVRISILVNGDPVDALSFIAHRAV
                     AEQRGRAICAKLKDLIPRQLFKIAVQAAIGGRVIARETISALSKDVTAKCYGGDITRK
                     RKLLEKQKEGKKRMRQFGKVEIPQSAFLAALKMDA"
     gene            complement(4223..5365)
                     /locus_tag="VFN42_04160"
     CDS             complement(4223..5365)
                     /locus_tag="VFN42_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HET8995838.1"
                     /translation="MIPRNFLISAASALAILGSVPAVAAPPITFGVQAPITGAYAEEG
                     QGIERAAKLLAEEQNAKGGLLGRKLEVTTCDDEGKAAQAAICARQLTNDHVIAVIGSY
                     TSGAALAAEPIYARANVLQSSDGTSDKLLERGYKTWFGNAPPNSAEAEFTAKYLVQVR
                     KFKKIAILTDHSSFATGLADAVEKDVKAVHGNVVDKAFINAGSQNFTPVLTKLKSMSP
                     DVIYFSGYYSDGGLIKAQMEQLGIKAVFVGGDANQNVAFAKIAGSAAAGAIIINVPAP
                     QDLPYPAAKEFVATYTKKYGAPPPSVYTLTNADGMRAVMQAIAHTKSTDPAKLENYLH
                     NLKQFEGLTGTFHWNQRGERIGSPFVAFAVESDGNYKILYPAPKAG"
     gene            complement(5550..>6230)
                     /locus_tag="VFN42_04165"
     CDS             complement(5550..>6230)
                     /locus_tag="VFN42_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HET8995839.1"
                     /translation="VNRNIIGAVVGVQPFGGHGLSGTGPKAGGPLYVRRLLAERPAET
                     GLPRGTAPEPFRQFLAFAGGGAAGFGDVTPYGVRMEFPGPVGEQNTYRLLPRGDVLCV
                     AATRAGALRQIAAALATGNRALVACTEEVPELDRLPPALHPHVLRAAPETDGRLVAAL
                     FEGDAGDLRRLSARLAEREGTIVPVFAAPSDDAADAVYPLEYLLAEQSISVNTAAAGG
                     NASLMTIG"
BASE COUNT         1048 a         2123 c         2033 g         1026 t
ORIGIN      
        1 agggcgagat catcgaaacc ggcatggtgg ccgacccccc cgtcgccgac ccggcgctgc
       61 agctccatat gccgcccttc cgccccaccc ccgccgatga cgagatcgaa cgcgctgccg
      121 ccgcgctgcg cagcgcctcg cgggtggtga tcgtcgcggg tgccggcgcc accgcatcgc
      181 aggccgggcc ggagctgctg gcgctgggcc agctgctggc cgcgccgatc gccacctcgc
      241 tgggtgcgcg cggcatcatc cccacgcagc accggctttc cgtcggctgc gccggcaatt
      301 acgccgcccc gccggccaat cagatcgtgc atgaggccga cctggtgctg ttcgtcggct
      361 gccacaccgg cgaccaggtc acccacacct ggcgcatccc gccgctcagc accccctgcg
      421 tgcagatcga catcgacccg ctggagctgg gccgcaccta tgccaacacg ctcgggctga
      481 tgggcgaccc gaaagcgacg ctggcgaagc tgatcgccgc gctcggcaag ctggaacgcg
      541 atacgtcctt cgcccaccgg gccgccggca tcgtcgccgc ctggcgggaa gcgcgcgccc
      601 cgctgctggc gcagaacgcg gtgccgatct accccgaccg gctatgcgcg gagatcaccc
      661 aggcgctgcc ggaagacggc atcctggtcg cggacaccgg ctattccggc atctggagca
      721 gcacgctgat cgaactgaac ggcacaggac agacctatct gcgtgccgcc ggctcgctgg
      781 gctggtcctt ccctgcggcg ctgggtgcga aatgcgcggc gccgcagcgg aaggtgatct
      841 gctggtcggg tgacggcgcg ctgtactatc acctgacgga actggagacc gcgcggcggc
      901 gcggcatcgc cgtggtgctg gtggtgaaca acaactccgg cttcggccag ggctggccga
      961 acatccagcg gcagcagggc aacaagccgg gcgacgtggc agagctggtg cgcttcggcc
     1021 cgacaaattt tgccgaggtg gcgcgcagct tcggcctgcg cggcatccgg gtggagcagc
     1081 cggcgcggat tgccccggcg ctgcgcgatg ccctcgcgtc cgatgagacg gtggtggtcg
     1141 atgtcgccac cgacattgac tgccgcgcgc cggagccgtg gctgccggcg ggttgagacc
     1201 ggcggcgggc gatgcgggcc ggccggctgg gaggcattca gtggatcgtg tcgcgctttc
     1261 agctgttcgc gaacgaacgg ctacagcgcg acctgcatcc gcacatcgaa cgagtcgtac
     1321 tccgcccgca ctccgtcctt gtcccggtcc agtagtccag ccgcttggag cgccgtcacg
     1381 tcttcatgca cgcgccgata gtcgcggccg agcgcgcgtg ccagatcccg aacgttcttc
     1441 gccgggtgct ggtggacgtg ccgcagcagc cccagccgct tgggcgacaa ggtgcggacc
     1501 atcgtctccc agctctcgaa tccgacatgc agttcggcgt tggccggcgt aagttcgcag
     1561 tgctccgccc gttgccaacc ttcgatgaat cgccggccgg cggtctccag ggtttcgccg
     1621 acatggacgt ggaccgtcct ggtcatccct tgtctccttc acgcattgcc ctgacggctg
     1681 cctcgaaatc cgcaatcagt ttctctggtg ttgagaactc gtaagagctt tccaccccca
     1741 ggatgtgctt gtggtccccc ttgccacgtt cgttgtcgaa caggaccaga cgcttcccgg
     1801 gcctgccaaa gaacagggcg tatttgaaag agtgcctcgc cggacggacc ggctggggaa
     1861 cccgccaaag ccagagcgcg atgaggaatc cgtcatccgt ctccaggcgg atgtcgagca
     1921 gcttttctgc cttcggcatg ccacccggct tcgatatggc ttactatgtc atagttggac
     1981 gcccatcagg tgtcaacagg accgttgttg ccctgggccg caggctatca ctgccccgca
     2041 taattggctg gcggcgaggg gaaaaggcaa tcagcgagac gcaagtcagc ccccggcgcc
     2101 ggcccgatgc cgcgacccgg atgacaccag ccttcggacc ctctatctcc tgcagcaacg
     2161 cagcccgcgc cccaggcaca cccaggggtc aggctcaccc gaccttagat caccggacat
     2221 gaccgacacc ccgatcgacc acatccgcaa cttcgcgatc atcgcgcata tcgaccacgg
     2281 gaaatccacc ctggccgacc gcctcatcca ggcgaccggc gcactgagcg accgtgagat
     2341 gaccgcccag gtcctggaca acatggagct ggagcgcgaa cgcggcatca ccatcaaggc
     2401 gcagacggtc cgcctgacct atccggccaa gaacgggcag gtttacgaac tgaacctgat
     2461 ggacacgccc ggccacgtgg atttcgccta tgaggtgtcc cgcagtctgg ccgcctgtga
     2521 gggcagcctg ctggtggtgg acgcctccca gggcgtggag gcgcagaccc tggccaatgt
     2581 ctaccaggcg atcgacgcca atcacgaaat cgtgccggtg ctgaacaaga ttgacctgcc
     2641 ggccgcggag ccggaacggg taaagcagca gatcgaggac gtgatcggtc tggacgcctc
     2701 cgacgcggtg gagatcagcg ccaagaccgg cctgaacatc gaaggcgtgc tggaggcgct
     2761 ggtgcaccgc ctgccgcccc cgaagggcga tggcaacgcg ccgctcaagg cactgctggt
     2821 ggatagctgg tacgaccagt acctcggcgt ggtgatcctg gtgcgggtga aggacgggcg
     2881 cctgaagcgg gcgcagaaga tccgcatgat gtccaccggc gcggtgcacc aggtggaaca
     2941 ggttggggtg ttctcgccca agatgatccc aaccgacgat ctcggcccgg gcgagatcgg
     3001 ctacatcacc gcggcgatca agaccgtggc cgactgcaac gtcggcgaca cgatcaccga
     3061 ggaccgcaat ccggccgccg aaccgctgcc gggattcaag ccgtcgatcc cggtggtgtg
     3121 gtgcggcctg taccctgtgg acgccgacga gttcgagaag ctgcgcgaat cgctcggccg
     3181 gctgcggctg aacgacgcca gtttccacta cgagccggaa acctccgccg cgctgggttt
     3241 tggcttccgc tgcggctttc tcggcctgct gcacctggag atcatccagg aacggctgac
     3301 ccgggagttc gacctggacc tgatcgccac cgcgccttcg gtggtgtacc acatcttcaa
     3361 gaccaacggc gagatgatgg agctgcacaa tccggtggac atgccggacg gcagcgtgat
     3421 cgaccacatc gaggaaccgt ggatccgcgc caccatcatg gtaccggacg actatctcgg
     3481 cgcggtgctg tcactgtgca ccgagcggcg cggcgtgcag gtggacctga cctatgtcgg
     3541 caaccgcgcc atggcggtgt accggctgcc gctgaacgaa gtggtgttcg atttctacga
     3601 ccggctcaag tcggtgtccc gcggctatgc cagcttcgac taccagatgg acggctacgc
     3661 cgaaggcgag ctggtgcgca tctccatcct ggtgaacggc gatccggttg acgcgctgtc
     3721 cttcatcgcc caccgggccg tggcagagca gcggggccgc gccatctgcg ccaagctgaa
     3781 ggatctgatc ccgcgccagt tgttcaagat cgcggtgcag gcggcgatcg gcggccgggt
     3841 gatcgcgcga gaaaccatca gcgcgctgag caaggatgtc actgccaagt gctatggcgg
     3901 cgacatcacc cgcaagcgca agctgctaga gaagcagaag gaaggcaaga agcgcatgcg
     3961 ccagttcggc aaagtggaga ttccacaatc cgccttcctc gccgcactga agatggacgc
     4021 atagcgggca atcactgcgt gatgaccggc gcaggccgcc accaccgcgt cacagccggc
     4081 gaaggccggt aacatctcgt cctggccggc agaccgggag cacctcgtct ggccggcgaa
     4141 ggccggcaat ccacgacttc gctgggctgc acgccgcagt gtcgtgagtg gcgggcctgc
     4201 gcccgccacg acctaacgac agtcagcccg ctttcggcgc cggatagaga atcttgtagt
     4261 tcccgtcgga ctccaccgcg aacgccacga acgggctgcc gatccgttca ccgcgctggt
     4321 tccagtggaa cgtgcccgtc agaccctcga actgcttcag attatgcagg tagttctcca
     4381 gcttcgccgg gtccgtgctc ttggtgtgcg cgatcgcctg catcaccgcg cgcatgccat
     4441 ccgcgttggt cagcgtgtac accgatggcg gcggcgcacc gtatttcttg gtgtaggtcg
     4501 cgacgaactc cttggccgcc ggatacggca ggtcctgcgg tgccggcacg ttgatgatga
     4561 tggcgcccgc cgccgcgctg cccgcgatct tggcaaatgc gacgttctgg ttggcgtcgc
     4621 cgccgacgaa caccgccttg atgccaagct gctccatctg cgccttgatc agcccgccgt
     4681 ccgaatagta gccggagaag tagatcacgt ccggggacat cgacttcagc ttggtcagca
     4741 ccggcgtgaa gttctgcgac ccggcgttga tgaaggcctt gtccaccacg ttgccgtgca
     4801 ccgccttcac gtccttctcc accgcgtcgg ccagaccggt ggcgaagctg gaatggtcgg
     4861 tcaggatggc gatcttcttg aacttccgca cctgcaccag gtatttcgcg gtgaactcgg
     4921 cttccgcgga gttgggcggg gcgttgccaa accaggtttt gtagccgcgc tccagcagct
     4981 tgtcgctggt gccgtcggag gattgcagga cgttcgcgcg tgcatagatc ggttcggccg
     5041 ccagcgcggc accgctggtg taggagccga tcacggcgat cacgtggtca ttggtcagct
     5101 gccgtgcgca gatcgccgcc tgcgctgcct tgccctcatc gtcgcaggtg gtgacctcga
     5161 gcttgcggcc gagcaggcca cccttggcgt tctgctcctc ggcgagcagt ttggcggcgc
     5221 gctcgatgcc ctggccttcc tcggcgtaag cgccggtgat cggcgcctgc acgccgaagg
     5281 tgatcggcgg cgccgccacg gcggggaccg agcccagaat ggccagcgcc gaggcggcac
     5341 tgatcagaaa attgcgcggt atcatacgtt tctcccgtgg ttgaagtgaa ggggtgcaag
     5401 gctgcgtgac gagactcctc cccctcccct tgcgggaggg ggacgggggg gaggggtggt
     5461 cgaggttgcc cgggaaaccc atgacagccc cctcccccca tccccccctc cgcaagggga
     5521 gggggcgtat tgagaattcc ccggctgtct catccgatcg tcatcaggct ggcattgcct
     5581 ccggcggcgg cggtattcac gctgatcgac tgttcggcca acaggtattc cagcggatag
     5641 acggcgtcgg ccgcatcgtc gctcggcgcg gcgaagaccg gcacgattgt cccctcgcgt
     5701 tcggccaggc gcgcgctcag gcgacgcaga tcgccggcat cgccctcgaa caatgcggca
     5761 accagcctgc catccgtctc cggcgcggcg cggagcacat gcgggtgcaa tgcaggcggc
     5821 aggcgatcca actccggcac ctcctccgtg caagcgacca gcgcgcggtt gccggtggcc
     5881 agagccgcag cgatctgccg cagcgcgccg gctcttgtcg ccgccacgca cagcacatcg
     5941 ccgcgcggca gcaggcgata ggtgttctgc tcgcccaccg gtcccggaaa ttccatccgc
     6001 acgccgtacg gagtcacgtc accgaacccc gccgcccctc cgccggcgaa cgccaggaac
     6061 tgacggaacg gttccggcgc cgtgccgcgc ggcaacccgg tttccgccgg acgttccgcc
     6121 agcagccggc gcacatacaa cggcccgccg gctttcggac cggtgcccga cagtccgtgt
     6181 ccgccgaagg gctgcacgcc caccaccgcg ccgatgatgt tgcggttgac
//
LOCUS       DASSZH010000195         3381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1657800, whole genome shotgun sequence.
ACCESSION   DASSZH010000195 DASSZH010000000
VERSION     DASSZH010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1657800"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..122)
                     /locus_tag="VFN42_04170"
     CDS             complement(<1..122)
                     /locus_tag="VFN42_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003622765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HET8995840.1"
                     /translation="MLKLALFFLVIALIAAVFGFTGIASAAVGIAKILFFIFLV"
     gene            complement(255..593)
                     /locus_tag="VFN42_04175"
     CDS             complement(255..593)
                     /locus_tag="VFN42_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995841.1"
                     /translation="MTSTKYVKTALAAACALGLAAAPAFAATGTSGMNSGTSGMTSGT
                     SSSLNSGSSMNHGKKLNNGSDSAMNTTGNNTGSSTATGATGSSSHPGTMGTNSLGGAG
                     GGSGSAGASK"
     gene            complement(780..1142)
                     /locus_tag="VFN42_04180"
     CDS             complement(780..1142)
                     /locus_tag="VFN42_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HET8995842.1"
                     /translation="MQRLWVGLGALAGLTAVGMAAVAAHGLDGLGPARLGMVRNALQM
                     QGWHALALLACGLWAPRGGWLADCAGAAFLLGLLLFCGAVYALAIAGAAVGILAPIGG
                     SLLMLGWALLGVSALRAR"
     gene            1231..2181
                     /gene="pip"
                     /locus_tag="VFN42_04185"
     CDS             1231..2181
                     /gene="pip"
                     /locus_tag="VFN42_04185"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HET8995843.1"
                     /translation="MPRGDLFPEIGPFETGYLPLSEGHVMYWEQVGHPRGRPALFLHG
                     GPGAGAGAVHRRFFDPDFWRVIIFDQRGAGRSRPLGSLEHNTTQDLVRDIEVLRRHLG
                     IDKWLLFGGSWGSTLALAYAQAHPDRVLGCVLRGVFLGRPQEVEWFLHGIARFYPDAH
                     HAFASFLPPEERDDLLGGYLRRLTHPDPAVHLPAARAWSVYEGSCSTLLPSPETVTSF
                     AQDRTALGLARIEAHYFAHDLFLPPQGLLDQMARISHIPAEVVQGRYDMICPAQTAFD
                     LAAAWPNARLSLVPDAGHSALEPGIRRALVAAVERFRHGR"
     gene            complement(2320..>3381)
                     /locus_tag="VFN42_04190"
     CDS             complement(2320..>3381)
                     /locus_tag="VFN42_04190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8995844.1"
                     /translation="ERLEAQRGAVDLVVLQGSLSGMAGEVFVRRLRSTPFGRRAVLLW
                     IGNEADGAAVDASVPSPPDPYQVAAVARQLLAERPSLEALEPNASLSRGGRVLLVEDD
                     KANQALLAAALTRRGFAVFTAGNGEEAVRMAGRDSFDAILMDLQMPGLDGFEATRRIR
                     ALPGHAGSVPIAALTALQGARLRQRCTETGFTAVLEKPVNMERLTDCLQRWVSDPGSP
                     SPVAIDQSAYVADVSVAFLEEMVAVVGLDRARACVAEFIAGATARCRRLGELLPGWEV
                     GAILRSCEEISGMAETCGALALGELLEEIADAASRDDREAAEGMIGRLDTVIARLPHA
                     MAACLGDVAQRWSRDSKAA"
BASE COUNT          480 a         1273 c         1124 g          504 t
ORIGIN      
        1 atgaccagga agatgaagaa caggatcttg gcgatgccga ccgcggccga ggcgatgccg
       61 gtgaagccga acaccgcggc gatcagcgcg atgacaagga agaacagggc cagtttcagc
      121 acgtgagcgg tttccttcga acgaggactc gaccacgtcc ggcgttgcca atgacgccgg
      181 acgatccgat tcggagaaaa tcggcatcag gcaggcagtc gggacgggcc gcggcccgtc
      241 cccccgtgcg gcctttactt cgaggcgccg gcgctgcccg aaccgccgcc ggctccgcca
      301 aggctgttgg tgcccatcgt gccgggatgg ctgctggagc ccgtggcccc ggtcgcggtg
      361 ctgctgccgg tgttgttgcc ggtcgtgttc atggcactgt cgctgccgtt gttcagcttc
      421 ttcccgtggt tcatcgagga gccgctgttc aggctgctgg aggtgccgct ggtcatgccg
      481 gaggtaccgc tgttcatgcc gctcgtgccg gtcgcagcga aggccggtgc cgccgccaga
      541 ccaagcgcac atgccgccgc cagggccgtc ttcacgtact tcgtgctcgt catgtcatgc
      601 tccattgttc tttcgcgccg cctgttggcc gcgcgccccg ggacaacgtc gcggcgcgct
      661 gacgcgttgg cgcgtgcaag ggtcaaccat ccgggattgc cggatagcat cgtcaatgca
      721 atcagacgtc cgccagccgg ggttttaggc gattccgttc cccggccggt ccgatcgcgt
      781 caccgcgccc gcagcgcgga cacgcccagc aatgcccagc cgagcatcag cagcgagccg
      841 ccgatcggcg ccaggatgcc caccgcggca ccggcgatcg ccagcgcata gaccgcgccg
      901 cagaacagca gcagccccag caggaacgcc gcaccggcgc agtcggccag ccagccgcca
      961 cgcggcgccc acaggccgca ggccagcagc gccagcgcgt gccagccttg catctgcagc
     1021 gcattgcgca ccatgcccag ccgggcaggc cccagcccgt ccagcccgtg tgcggccacc
     1081 gccgccatgc ccaccgccgt caatccggcc agcgcgccca gcccaaccca gagtctctgc
     1141 atgcatcctg cctcgtgatc gcggcgaacc ggcaacagtg gggtctccgg cctgttccgt
     1201 ccggccgcag ccgcttatac gatgcgtgcc atgccgcgcg gtgatctgtt ccctgaaatc
     1261 ggccccttcg agaccggcta tctgccgttg tccgagggcc acgtgatgta ctgggagcag
     1321 gtgggccacc cgcgtggccg gccggcgctg ttcctgcacg gcggtccggg ggccggcgcc
     1381 ggcgcggtgc accgccgctt cttcgacccc gatttctggc gcgtcatcat cttcgaccag
     1441 cgcggcgccg gccgctcccg cccgctcggc agcctggagc acaacaccac gcaggacctg
     1501 gtgcgcgaca tcgaagtgct gcgccggcac ctgggcatcg acaaatggct gctgttcggc
     1561 gggtcctggg gctccaccct ggcgctggcc tatgcccagg cgcatccgga ccgggtgctg
     1621 ggttgcgtgc tgcgcggcgt gttcctgggc cgcccgcagg aggtggagtg gttcctgcac
     1681 ggcatcgccc gcttctaccc ggatgcgcac cacgccttcg ccagtttcct gccgccggag
     1741 gagcgggacg acctgctggg cggctacctg cggcggctga cccatccgga tcccgccgtg
     1801 cacctgcccg ccgcccgggc ctggtcggtc tatgaaggct cctgcagcac actgctgccg
     1861 agccccgaga ccgtgacgtc cttcgcgcag gatcgcaccg cgttggggct ggcgcggatc
     1921 gaggcgcact atttcgcgca cgacctgttt ctgccgccgc aggggctgct ggaccagatg
     1981 gcgcgcatca gccacatccc ggccgaggtg gtgcagggcc gctacgacat gatctgccca
     2041 gcgcagacgg cgttcgacct tgcggcggcc tggccgaacg cgcggctgtc gctggtgcct
     2101 gatgccggac actcggcgct ggaacccggt atcaggcgcg ccctggtggc ggcggtggag
     2161 cggttccggc acggccggtg agcgagacca gccacaccct caccgtggcg ctcaacgtga
     2221 cgaccccgtc gtgcgatggg ccatcgcgga gcgtcaggtc aggtcaggga cgctcgtctt
     2281 ctttctcatc gcacgtcact tccatcagaa cgccggcggt tacgccgcct tgctgtcccg
     2341 cgaccagcgc tgcgccacgt cgcccaggca cgccgccatc gcatgcggca ggcgcgcgat
     2401 cacggtgtcc agccgcccga tcatcccctc cgccgcctcg cggtcgtcgc gggatgcggc
     2461 gtcggcgatc tcctccagca gctcgcccag cgccagcgcg ccgcaggtct ccgccatccc
     2521 gctgatctcc tcgcagctcc gcaggatcgc gccaacctcc cagcccggca gcagctcgcc
     2581 caggcggcgg cagcgggccg tcgcaccggc aatgaactcc gccacgcagg cgcgcgcacg
     2641 gtccagcccg accaccgcga ccatctcctc caggaacgcc accgacacat cggcgacata
     2701 ggcggactgg tcgatcgcca ccgggctggg ggagccggga tcgctaaccc agcgctgcag
     2761 gcagtcggtc agccgctcca tgttcaccgg cttctccagc acggcggtga agccggtctc
     2821 ggtgcagcgc tggcgcaggc gggcgccctg cagcgccgtc aacgcggcga tcggcacgct
     2881 gccggcatgg ccgggcaggg cgcggatgcg gcgggtggcc tcgaacccgt ccagtccggg
     2941 catctgcagg tccatcagga tcgcatcgaa gctgtcgcgg ccggccatgc gcaccgcttc
     3001 ctcgccgttg cccgcggtga acaccgcgaa gccgcggcgg gtcagcgcgg cggccagcag
     3061 cgcctggttc gccttatcgt cctccaccag cagcacgcgt ccgccgcggc tcagcgaggc
     3121 attgggctcc agtgcctcca gcgacggccg ttccgccagc aactgccgcg ccaccgcggc
     3181 cacctggtag ggatcgggcg gtgacggcac gctggcgtcc accgccgcgc cgtcggcctc
     3241 gttgccgatc catagcagca ccgcgcgccg gccgaatggc gtgctgcgca gccggcgcac
     3301 gaacacttcg ccggccatgc ccgacaggct gccctgcagc accaccagat cgaccgcgcc
     3361 gcgctgcgcc tccagccgct c
//
LOCUS       DASSZH010000196         6868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1692181, whole genome shotgun sequence.
ACCESSION   DASSZH010000196 DASSZH010000000
VERSION     DASSZH010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6868
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1692181"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VFN42_04195"
     CDS             <1..541
                     /locus_tag="VFN42_04195"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET8995845.1"
                     /translation="GRFPRLDRWTTMRGMAPGRGRWRSAIRQGGTTVPNTVRLHRVLA
                     TRPEKVYRAFLEPDALAKWLPPNGFVCTVDHLEAKVGGTFRMAFRNFTTGNSTAFHGR
                     YLELVPGERLRYTDAFEDPNLPGEIQVTVTLKKVLVGTEIDIVQSNLPHVIPPEACYL
                     GWQESLRNLARLVEPEITQ"
     gene            682..1449
                     /locus_tag="VFN42_04200"
     CDS             682..1449
                     /locus_tag="VFN42_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET8995846.1"
                     /translation="MTDHLLTTRTGKVLEVRLNRPEKKNALTRAMYKGMADAFETADR
                     DPNIRVVLLTGTGDTFTSGNDIKDFQARAAEGTEGAGSRFLPVISTMQTPLVAAVNGA
                     AVGVGTTMLAHCDLIVAARSARFVMPFTSLGLVPEAGSSLLFPRLLGPQRASALLLLG
                     EPLDADTALQWGFVNRVVDAASLMQTAREMAQRLAALPPEAVRLTKRLIRHGTPDLAG
                     RIAEELKLFAERLTSPEAKEAFAAFVEKRAPDFSRIG"
     gene            1457..2260
                     /locus_tag="VFN42_04205"
     CDS             1457..2260
                     /locus_tag="VFN42_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HET8995847.1"
                     /translation="MIDLDGYFARIGYTGPRRATLETLGMLHALHPAAIPFENLDPLL
                     GRPVLLDLGSLQDKLVRRRRGGYCFEQNTLFRAALEALGFGVTSLAARVLWGSKPGRP
                     HPRTHMLLLVHVDGRDYIADVGFGGRLMATPLQRVPGLEQPTQHGMMRLVEHGMFLTL
                     QAASDDGWEDLYRFTLEPQFPIDQETANWFTATHPDSLFRHNLLMERLAPDLRCSLFN
                     RRLTQRYPDGRVSLTQLADAAALRRVLTEAFALDVPAKLDAVFARLPAG"
     gene            2329..3318
                     /gene="gshB"
                     /locus_tag="VFN42_04210"
     CDS             2329..3318
                     /gene="gshB"
                     /locus_tag="VFN42_04210"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HET8995848.1"
                     /translation="MPRQLKVAVQMDPIESINIDADSTFALMLEAQQRGHALWHYEVR
                     HMALREGVKRQGARREERLFARARPVTVQRVRGSHYTFGEPVLLDLGGTDVVLMRQDP
                     PFDMAYITATHMLEHIQPKTLVVNDPAAVRNAPEKLLVTHFPDLMPPTMITWDLEAIR
                     SFRREHKDIIVKPLFGNGGAGVFRIKPDDENLASLLEMHFARSREPLMFQRYEPAVRQ
                     GDKRIILVDGEPMGVVNRVPAQGEARSNMHVGGRPEKATLTARDREICAAIGPTLREQ
                     GLVFVGIDVIGDWLTEINVTSPTGIQEIDRFDGINIEAAIWDRIEAKLAARVA"
     gene            3439..4602
                     /locus_tag="VFN42_04215"
     CDS             3439..4602
                     /locus_tag="VFN42_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HET8995849.1"
                     /translation="MPWREVSRMEGREEFVSLAMADGANVALLCRRFGISRKTGYKWL
                     ARHRTGAELIDRSRRPLASPLRTSPEMEAAVVALRQQRPSWGGRKLRRRLLDQGYQAV
                     PAASTIGAILGRHDQIDAAEAVKHTPFRRFEHDRPNALWQMDFKGHFAHAAGRCHPLT
                     VLDDHSRYAVCLTACADETAGTVRERLIAVFRRYGLPERINVDNGSPWGDGAGGRYTM
                     MTVWLLRLGLRCSHSRPYHPQTNGKDERFHRTLKRDVLYGRSYGDLTACQRAFDDFRI
                     IYNGERPHEALGFAVPASRYCASTREYPETLPPIEYDPADQVRQVQQGGRVSFQGREL
                     HLPKAFAGQPIALRPTTTDGIWTAVFVAQRIAQIDLRDALHPVQPVTHVPEHV"
     gene            4729..6168
                     /gene="tldD"
                     /locus_tag="VFN42_04220"
     CDS             4729..6168
                     /gene="tldD"
                     /locus_tag="VFN42_04220"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HET8995850.1"
                     /translation="MTALAATDRLFFERADAALSREAAERRLQQALTNSDDGELFLEY
                     RESESISLDDGRIRSAGFDTTLGFGLRAVAGEATGYAHAGELSDDALERAAASVAAVA
                     AGHSGTAAEPPRATNSRLYSDANPLLATPFGSRTELLGEIDAYARGKDSRVKQVMASI
                     SGEWQAVQIMRADGTRVADLRPLVRLNVAVVMEQNGRRETGSHGMGGRQAYDIVTRPD
                     AWRGAVDEAIRQAIVNLDSRPAPAGEMEVVLGAGWPGILLHEAIGHGLEGDFNRKQTS
                     AFAGLLGKQIAAKGVTVVDDGTMPDRRGSLTVDDEGTPSSRTVLIEDGILVGFMQDRL
                     NARLMGMRATGNGRRQSFAHAPMPRMTNTVMLGGGHTPAEMISSVKRGLFAVNFGGGQ
                     VDITSGKFVFSASEAYLIEDGKVTAPVKGATLIGNGPDALTKVAMIGNDMALDPGVGT
                     CGKNGQGVPVGVGQPTLKLTGLTVGGTAT"
     gene            complement(6177..6863)
                     /gene="lipB"
                     /locus_tag="VFN42_04225"
     CDS             complement(6177..6863)
                     /gene="lipB"
                     /locus_tag="VFN42_04225"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HET8995851.1"
                     /translation="MNDSASIGLTTPEWRIAEGLTAYPDAMALMQDRVAAIRAGSAGE
                     LVWLVEHPPLYTAGTSAQASDLTDPARFPTYSAGRGGQWTYHGPGQRTGYVMLDLNRP
                     HGSVPARDVRGFVHGLEEWLIRALDRFNVRGERREGRVGIWVMDPRIGEAKIAAIGVR
                     VSRWVTWHGVALNVDPDLAHFGGIVPCGVREHGVTSLAALGITATMAEADTALRAAWD
                     AVFGGSAPLP"
BASE COUNT         1158 a         2283 c         2379 g         1048 t
ORIGIN      
        1 cggccggttc ccccggctgg atcgttggac cacaatgcgc ggcatggccc ccggaagagg
       61 tcgctggcgt tcggccattc gacaaggagg aaccaccgtg cccaataccg tgcgtttgca
      121 ccgcgtcctg gcaacccgtc ccgagaaggt ataccgcgcc ttcctggagc cggacgcgct
      181 ggcaaaatgg cttccgccca acggctttgt ctgcaccgtc gatcacctgg aagcgaaggt
      241 cggcggtact ttcaggatgg ccttccggaa tttcacgacc ggcaacagca ctgcattcca
      301 cggtcgctat ctcgaactcg tgccgggcga acgcctgcgc tacaccgacg cgttcgagga
      361 ccccaacctc cctggtgaaa tccaggtgac ggtgacgttg aaaaaggtcc tggtcggcac
      421 cgagatcgac atcgtccagt ccaaccttcc gcacgttatc ccgccggagg catgctatct
      481 cggatggcag gaatcactgc ggaacctggc aaggctggtc gaaccggaaa tcacgcaata
      541 gccggccacc gcctcgatgg cgtcaatcac cagccgggtg cgcggcgcgt agcgcgcagg
      601 aacacgggca tgccgcgatc gcaagccggg cagcacctcg ctggtggtgc gcctgaacag
      661 gccgacaagg gagggccgtg aatgaccgac cacctgctca ccacccgcac cggcaaggtg
      721 ctggaggtcc ggctcaatcg gccggagaag aagaacgcgc tgacccgagc catgtacaag
      781 ggcatggcgg acgccttcga aaccgccgac cgcgatccga atatccgggt cgtgctgctg
      841 accggcaccg gcgacacctt cacctccggc aacgacatca aggatttcca ggcccgggcc
      901 gccgaaggca ccgagggtgc gggatcgcgc ttcctgccgg tcatctccac catgcagacg
      961 ccgctggtcg cggcggtcaa cggcgccgcg gtgggtgtcg gaaccaccat gctggcgcat
     1021 tgcgatctga tcgtcgccgc acgctccgcc cggttcgtca tgcccttcac cagcctgggc
     1081 ctggtgccgg aggccggcag ctcgctgctg ttcccccgcc tgctgggccc gcagcgcgcc
     1141 agcgccctgc tgctgctggg cgagccgctg gacgcggaca ccgcgctgca atggggcttc
     1201 gtcaaccgcg tggtggacgc tgcgtcactg atgcagacgg cccgggagat ggcgcaacgg
     1261 ctggcggcct tgccgccgga agcggtgcgg ctgaccaaac ggctgatccg ccacggcacg
     1321 cccgacctgg ccggacgcat cgcggaggaa ctgaagctgt tcgcggaacg gctgacatca
     1381 ccggaagcga aggaagcctt cgccgccttc gtggaaaagc gtgcgcctga cttctcgcgc
     1441 atcggatgac ccccgcatga tcgacctgga cggctatttc gcccgaatcg gttacaccgg
     1501 gccgcgccgc gccacgctgg aaacgctcgg catgctgcat gcgctgcatc cggccgccat
     1561 tccgttcgag aatctcgacc cgctgctcgg gcgcccggtg ctgctggacc tgggctcgct
     1621 gcaagacaag ctggtgaggc gacggcgtgg cggatattgc ttcgaacaga acacgctgtt
     1681 ccgtgcggcg ctggaagcgc tgggattcgg tgtgaccagc ctggcggcac gtgtgctgtg
     1741 gggcagcaag ccggggcgcc cgcatccccg cacgcatatg ctgctgctgg tgcacgtgga
     1801 cggccgcgac tacatcgccg atgtcggttt cggcggccgg ctgatggcga cgccgctgca
     1861 acgggtgcca gggctggaac agccgacgca gcacggcatg atgcggctgg tcgagcatgg
     1921 catgttcctc accttgcagg ccgccagcga tgacggctgg gaggatcttt accgcttcac
     1981 cctggagccg cagtttccga tcgaccagga aaccgccaac tggttcaccg ccacgcatcc
     2041 ggattcgctg ttccgccaca atctgctgat ggagcggctg gccccggatc tgcgctgcag
     2101 cctgttcaac cgccggctga cccagcgcta tccggacgga cgggtgagcc tgacacagct
     2161 ggcagacgct gccgcactca ggcgtgtgct gaccgaggct ttcgctctgg acgtgccggc
     2221 caaactggac gcggtgttcg cccggctgcc ggcgggctga cggcggctct ggccatccgt
     2281 cccgtctgcc catagacagg gcggcaacgc aagcaatggg ggccagccat gccacggcaa
     2341 ttgaaggtcg cggtccagat ggacccgatc gagtccatca acatcgacgc cgattccacc
     2401 ttcgccctga tgctggaggc gcagcagcgc ggccacgcgc tgtggcacta cgaggtgcgc
     2461 cacatggcgt tgcgcgaggg cgtgaaacgg cagggtgcgc ggcgggagga gcggctgttc
     2521 gcacgtgcgc ggccggtgac cgtgcagcgg gtacgcggca gccattacac attcggcgag
     2581 ccggtgctgc tggatttggg cggcacggat gtggtgctga tgcggcagga tccgccgttc
     2641 gacatggcgt acatcaccgc gacgcacatg ctggaacaca tccagccgaa gacgctggtg
     2701 gtgaacgacc cggcggcggt gcgcaatgcg ccggaaaagc ttctggtgac gcatttcccc
     2761 gacctgatgc cgccgaccat gatcacctgg gacctggagg cgatccgcag cttccgccgg
     2821 gaacacaagg acatcatcgt caagccgctg ttcggcaatg gtggcgccgg cgtgttccgc
     2881 atcaagccgg atgacgagaa cctggcctcg ctgctggaga tgcacttcgc ccgctcgcgc
     2941 gagccgctga tgttccaacg ctacgagccg gcggtgcggc agggcgacaa gcgcatcatc
     3001 ctagtggatg gcgagccgat gggcgtggtg aaccgcgtgc cggcgcaggg cgaggcacgc
     3061 agcaacatgc atgtcggcgg ccggcccgag aaggccacgc tgaccgcgcg cgaccgggag
     3121 atctgcgcgg ccatcggccc gacgctgcgc gaacaggggc tggtgttcgt cggtatcgac
     3181 gtgatcggcg actggctgac cgagatcaac gtgacgtcgc cgaccggcat ccaggagatc
     3241 gatcggttcg acggcatcaa catcgaagcg gcgatctggg accgcatcga ggcgaagctg
     3301 gccgcgcgag tggcttaggc aacaggcaac ctcctccccc tccccttgtg ggtgtacaga
     3361 ccggggagat gggtgacagg tgttcgggga catgggtaac acaatgggac catggaatgg
     3421 gtgcggggag atcgcgtcgt gccatggcga gaagtgtcac gcatggaagg ccgtgaggaa
     3481 tttgtgagct tggcaatggc ggacggcgcg aatgttgcct tgctgtgccg gcggttcggc
     3541 atcagccgca agaccggcta caagtggctg gcccggcatc ggacgggtgc cgagctgatc
     3601 gatcggtcgc gccgcccgct ggccagcccg ttgcgcacct cgcccgagat ggaggcggcg
     3661 gttgtggcgc tgcgtcagca gcggccgagt tggggcggac ggaagctgcg tcgccggttg
     3721 ctcgaccagg gctaccaggc ggttcccgcg gcatcgacga ttggcgccat cctgggccgc
     3781 cacgaccaga tcgatgcggc ggaagcggtg aagcacacgc cgttccggcg gttcgagcat
     3841 gatcggccga acgcgctgtg gcagatggac ttcaaagggc atttcgcgca tgccgccggc
     3901 cgctgtcatc cgctgacggt cctggatgac cacagccgct acgctgtttg ccttaccgcc
     3961 tgtgccgacg agacggccgg gacggtccgc gagcggctga tcgcggtatt ccgacgctat
     4021 ggcctgcccg agcggatcaa tgtggacaac ggcagcccct ggggagatgg cgcgggcggg
     4081 cgctacacca tgatgaccgt gtggctgctg cggctcggcc tgcgatgcag tcatagccgg
     4141 ccctatcatc cgcagaccaa tggcaaggac gagcggttcc accgtacgct gaagcgcgat
     4201 gtactgtatg gccggagcta tggcgacctg acggcgtgtc aacgcgcgtt cgacgacttc
     4261 cgcatcatct ataatggtga acgtccgcat gaggcgctgg gctttgccgt gccggccagc
     4321 cgctactgcg ccagcacgcg ggagtacccc gagacgctgc cgccgatcga gtacgatccg
     4381 gctgaccagg tgcggcaggt tcagcagggc ggccgggtca gcttccaggg tcgcgagctg
     4441 caccttccca aagcctttgc cggccagcca atcgctctgc gtccaaccac gaccgatgga
     4501 atatggaccg cagtctttgt cgcgcagcgc atcgcgcaga tcgatctgcg cgatgcgctg
     4561 cacccagtgc aacctgttac ccatgtcccc gaacatgtgt aacccttgtc tccggtctaa
     4621 acacccgcaa ggggaggggg agtcccgtga ccatcccacg atccgcgctg gcaaagccgg
     4681 gcgttcgacc ccacactcag ggaagcactc caaccacttg aggctgccat gactgcgctc
     4741 gccgccaccg acagactgtt cttcgagcgg gccgacgccg cgctgagccg cgaagccgcg
     4801 gaacgccggc tgcagcaggc actgaccaac agcgacgacg gcgagctgtt cctggaatat
     4861 cgcgagagcg agtcgatcag cctggatgac ggtcgcatcc gcagcgccgg cttcgacacc
     4921 acgctgggct tcggcctgcg ggcggtggcg ggcgaggcca cgggctacgc ccatgccggc
     4981 gagctgtcgg acgatgcgtt ggaacgcgcc gctgccagtg ttgccgcggt ggctgccggg
     5041 cattccggca ccgccgcgga gccgccgcgc gccaccaatt cccgactgta ttccgacgcc
     5101 aacccgctgc tcgccacccc cttcggcagc cgcaccgagc tgctgggcga gatcgacgcc
     5161 tatgcccgcg gcaaggattc gcgggtgaag caggtgatgg cctcgatctc cggcgaatgg
     5221 caggcggtgc agatcatgcg cgccgacggc accagggtcg ctgatctgcg cccgctggtg
     5281 cggctgaacg tcgccgtggt gatggaacag aacggccggc gggaaaccgg gagccacggc
     5341 atgggcggcc ggcaggccta tgacatcgtc acccggccgg atgcctggcg cggcgcggtg
     5401 gacgaggcga tccgccaagc catcgtcaac ctggactcgc gtcccgcccc ggccggcgag
     5461 atggaggtgg tgctgggggc cggctggccc ggcatcctgt tgcacgaggc gatcggccac
     5521 gggctggagg gcgacttcaa ccgcaagcag acctcggcct tcgccgggct gctgggcaag
     5581 cagatcgccg ccaagggcgt caccgtggtg gatgacggca ccatgcccga tcgccgcggc
     5641 agcctgacag tggacgacga gggcacgccg tccagccgca cggtgctgat cgaggacggc
     5701 atcctggtcg gcttcatgca ggaccggctg aacgcgcggc tgatgggcat gcgcgccacc
     5761 ggcaacgggc ggcgccagtc cttcgcccat gcgccgatgc cgcgcatgac caacaccgtg
     5821 atgctgggcg ggggccacac gccggcggag atgatcagtt cggtgaagcg tgggctgttc
     5881 gcggtgaatt tcggcggtgg ccaggtggac atcacgtcgg ggaagttcgt gttctccgcc
     5941 agcgaggcct acctgatcga ggacggcaag gttactgccc cggtcaaggg cgccacgctg
     6001 atcggcaacg gcccggacgc gctgaccaag gtggcgatga tcggcaacga catggcgctg
     6061 gacccgggcg tgggcacctg cggcaagaac ggccagggcg tgccggtggg cgtcggccag
     6121 ccgacgctga agctgaccgg cctgacggtg ggcggcaccg cgacctgaag gcggcgtcag
     6181 ggaagcggag ccgaaccgcc gaacaccgcg tcccaggcgg cgcgcaaggc ggtgtccgcc
     6241 tcggccatcg tcgcggtgat gcccagcgcg gccaggctgg tcacgccgtg ctcccgcacg
     6301 ccgcagggca cgatcccgcc gaaatgcgcc aggtcgggat cgacgttcag cgccacgccg
     6361 tgccaggtca cccagcgcga cacgcgcacg ccgatcgcgg cgattttcgc ctcgccgatg
     6421 cgcgggtcca tgacccagat gccgacccgg ccctcccgcc gttcgccgcg cacgttgaag
     6481 cggtccagcg cgcggatcag ccactcctcc agcccgtgca cgaagccgcg cacgtcgcgc
     6541 gccggcacgc tgccgtgcgg ccggttcaga tccagcatga catagccggt gcgctggccg
     6601 ggtccgtgat aggtccattg cccgccgcgg cccgcgctgt aggtgggaaa gcgggccgga
     6661 tcggtcagat cggacgcctg ggcggaggtg ccggcggtgt acagcggcgg gtgctccacc
     6721 agccacacca gctcgccggc cgagccggcg cggatcgccg ccacccggtc ctgcatcagg
     6781 gccatcgcgt cggggtaggc tgtgagcccc tcggcgatgc gccattccgg ggtggtgagg
     6841 ccgattgaag cgctgtcgtt catccgct
//
LOCUS       DASSZH010000197         3009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1027437, whole genome shotgun sequence.
ACCESSION   DASSZH010000197 DASSZH010000000
VERSION     DASSZH010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027437"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2960)
                     /locus_tag="VFN42_04230"
     CDS             complement(<1..2960)
                     /locus_tag="VFN42_04230"
                     /inference="COORDINATES: protein motif:HMM:NF024516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HET8995852.1"
                     /translation="MRLHPIRRLTRYTLAILHWLAHLSHGVLVLALVLLAGGAWRLAQ
                     GPVNVSWLTTRLEASLNTPDSPIRLHIGKTALAWEGFRLGVDHPIDLRLTDVRLTDAA
                     RHQSLTVPQAELSLSLGALLIGRVQPRAIELDRARLAFRRAKDGTITLDLGDFAQPSA
                     GRSGDPLPALLAELARPPATDRTALRGWVSQIRRVRIRNAIVTVQDLQLGVTWRAPRA
                     EIDLVRNPHGGIAGSADLDLALGDQHARLRITEALASAATRTELTARISPLVPAALGR
                     LVPRFAALSAFDAPVDAQAHLQLGPQLRLQHARVTLHAGSGTVRIGQSAIPIVSAALT
                     AQGTPDAVQIESAEAKLQARPDSPVSTVQLTGTLHRNATAIDATLALGLDQVDFADLP
                     TLWPKGVSDGARAWVTRNITAGSAQNADIQVALTAKDDLSGITVTSATGTLDGSGLTV
                     HWLRPVPPIEDGKAQLRILGPDSLDIAVLGGKQELRGVPGRLTIQSGNMRITGLTEPE
                     QIGTIQAAVSGSLPDALALLREPRLNLLSTHPMPLQNPAGDVSASVSLRLPLQADVSM
                     DAVALRTSAHLNAVHLDKLIAGRSLDRASLDLVATQDDMTLKGQGLLAGIQTQFDGAM
                     DFRAGGPTQVLQRVSVSGRPSAKQLAAAGLDATDLLSGPVPLQADFIERRNGQSELSV
                     QADLTPATLAVEALDWRKPPGHFATADATLRLAGQRLLRIDRFTVNGSGISVQGSADY
                     SDGRISLVRLNRLEVGRNDLRGTIRLPPDRLSPIVADMSGRTLDLAARLERTQPPRPA
                     ATAEPPAGPPWTLNAQLDRVLTANKTMLERVKVQAENDGRVFRRLDVTGDTREHGAFA
                     LRITPEAGGRKLAATAAQAGDLLRALDWEKTVQGGRLSVTGTYDDSKPGHPLTATATI
                     SNFRVREAVGLGKLLQAMTLYGLVDALRGPGLSFNQAIIPLHLTSHSIEINGARAFSS
                     SLGLTAKGRIDLDA"
BASE COUNT          416 a         1041 c         1070 g          482 t
ORIGIN      
        1 tccgcgtcca ggtcgatccg ccctttcgcg gtcaggccga gcgatgaact gaacgcgcgt
       61 gcgccgttga tctcgatgct gtgcgaggtc aggtgcaacg ggatgatcgc ctggttgaac
      121 gacagcccgg gtccgcgcag cgcgtccacc aggccgtaca gcgtcatcgc ctgcagcagc
      181 ttgcccaggc ccaccgcctc gcgcacgcgg aagttgctga ttgttgccgt tgcggtcagc
      241 gggtggcccg gcttgctgtc atcgtaggtg ccggtcaccg acaggcgtcc gccttgcacg
      301 gtcttctccc aatccagcgc gcgcagcagg tcgcccgcct gcgccgcggt ggcggccagc
      361 ttgcgcccgc cggcctccgg cgtgatgcgc agtgcgaagg ccccgtgctc gcgcgtgtcg
      421 ccggtgacgt ccaggcggcg gaacacgcgg ccatcgttct ccgcctgtac cttgacgcgc
      481 tccagcatcg tcttgttggc cgtcagcacg cgatccaact gtgcgttcaa tgtccagggc
      541 gggccggcgg gcggctccgc cgtggcagcc gggcgtggcg gctgcgtgcg ttccagccgt
      601 gctgccaggt ccagcgtgcg ccccgacatg tcagccacga tgggcgatag ccggtccggc
      661 ggcagccgta tggtcccgcg caggtcgttg cgccccactt cgagccggtt tagacggacc
      721 agggaaattc gcccgtcgct gtagtcggca ctgccttgca ccgagatgcc gctgccgttc
      781 acggtgaacc ggtcgatgcg cagcagccgc tgcccggcca gccgcagggt cgcgtctgcg
      841 gtggcgaagt ggccgggcgg cttgcgccaa tccaatgcct ccaccgccag ggtggccgga
      901 gtcagatcgg cctgcaccga cagctcgctc tggccgttgc gccgctcgat gaaatcggcc
      961 tgcaacggca ccggcccgga cagcaggtcg gtggcgtcca ggccggccgc ggcaagctgc
     1021 tttgcgcttg gccggccgga gacgctgacc cgctgcagca cctgggtggg accgccggca
     1081 cggaaatcca tggcgccgtc gaattgcgtc tggatgccgg ccagcaggcc ctgtcccttc
     1141 agcgtcatgt cgtcctgcgt cgccacaagg tccaggcttg cccggtccag cgaacgcccg
     1201 gcgatcagct tgtccaggtg caccgcgttc agatgcgccg acgtgcgcag ggcaaccgcg
     1261 tccatcgaca cgtcggcttg cagcggcagg cgcagcgata cgctggccga tacgtctccg
     1321 gcggggttct gcagcggcat cggatgtgtg ctgagcaggt tcagccgcgg ttcgcgcagc
     1381 agcgccagcg catccggcag cgaaccggag accgcagcct ggattgtgcc gatctgctcc
     1441 ggctccgtca ggccggtgat gcgcatgttg ccgctttgga tcgtcagccg gccgggcacg
     1501 ccgcgcagtt cctgcttgcc gccgagcacc gcgatatcca gcgaatccgg accgaggatg
     1561 cgaagctgcg ctttgccatc ttcgatcggc ggcaccggcc gcagccagtg caccgtcagc
     1621 ccgctgccat ccagcgtgcc ggtggcgctg gtcaccgtga tgccggacag atcgtccttc
     1681 gcggtcagcg cgacctggat atcggcattc tgcgccgacc cggcggtgat gttgcgggtg
     1741 acccaggcgc gcgcgccatc ggagacaccc ttcggccaga gggtcggcag atcggcaaag
     1801 tccacctggt ccagtccaag cgccagcgtc gcgtcgatcg ccgtggcgtt ccggtgcagc
     1861 gtgccggtca gttgcacggt gctgaccgga ctgtccggac gggcttgcag cttcgcttcg
     1921 gccgactcga tctgcacggc gtccggcgtg ccctgggcag tcagcgccgc cgagacgatg
     1981 ggtatggcag actggccgat ccgcaccgtc cccgatccgg cgtgcagggt gacgcgtgcg
     2041 tgctgcaggc gcagttgcgg cccgagctgc agatgcgcct gcgcatcgac cggagcgtcg
     2101 aacgccgaga gtgcggcgaa gcgcggcacc agccgcccga gtgctgccgg cacgagcggg
     2161 ctgatccggg cggtcagctc cgtccgggtg gcggcggaag ccagcgcttc ggtgatccgc
     2221 agccgtgcat gctgatcgcc gagcgcgagg tcgaggtccg cggaacccgc gatgccgcca
     2281 tgcggattgc ggaccagatc gatttccgcc cgcggtgcgc gccaggtgac cccgagttgc
     2341 aggtcctgca ccgtcacgat ggcgttgcgg atgcgcacgc gtcggatctg gctgacccag
     2401 ccgcgcagcg cggtgcgatc ggtggccggc gggcgcgcca gttcggccag cagggcaggg
     2461 agcggatcgc cgctccggcc ggcggatggc tgggcgaagt cacccaggtc cagggtgatg
     2521 gtgccgtcct tggcgcgccg gaacgccagc cgcgcgcggt cgagttcgat cgcgcgcggc
     2581 tgtacccggc cgatcagcaa tgccccgagc gagagcgaca gttcggcctg tggtactgtc
     2641 aggctttgat gccgcgccgc gtcggtcagg cggacatcgg tcagccgaag gtcgatcggg
     2701 tggtcgacgc ccaggcggaa cccttcccag gccagcgcgg tcttgccgat atgcaggcgg
     2761 atcgggctgt caggcgtgtt gaggctggct tccagccggg tggtcagcca ggacacgttc
     2821 accggcccct gcgccagccg ccacgcgccc ccggcgagca gtaccagcgc cagtaccagc
     2881 accccgtgcg acaggtgcgc cagccaatgc aggatagcca gggtgtagcg ggtcagccgc
     2941 cggatcgggt ggagtctcat gacggcatgc gcggctgtac actccccctc cccttgcggg
     3001 cttggtccg
//
LOCUS       DASSZH010000198         6547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1504831, whole genome shotgun sequence.
ACCESSION   DASSZH010000198 DASSZH010000000
VERSION     DASSZH010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6547
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504831"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2067)
                     /locus_tag="VFN42_04235"
     CDS             complement(<1..2067)
                     /locus_tag="VFN42_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HET8995853.1"
                     /translation="MDLTRDVVADADHVPADAHGSASGAAATAGGEGFDLPRLLQAMQ
                     AMRAGDFSVRLPTDCTGLEGKICDTFNDIVASNQRMARELEQVGQVVGREGKTRTRVK
                     LGLSDGAWREMETSVNTLIDDLLWPTTAVTQAIAAVAQGDLLQTVRLDVDGRPLQGEF
                     LRLATIVNTMIKQLSVFTSEVTRVAREVGTDGKLGGQAQVSEVTGVWKDLTESVNSMA
                     SNLTAQVRNIAEVTIAVANGDLSKKITVDVRGEILQLKEAINTMVDQLRSFASEVTRV
                     AREVGTDGKLGGQALVPGVAGTWKDLTDSVNAMCGNLTDQVRNIAQVTTAVARGDLSR
                     KITVNVSGEILELKDTINTMVDQLNGFASEVTRVAREVGTEGRLGGQAQVPGVAGTWK
                     DLTDSVNSMAGNLTGQVRNIAEVATAIAQGDLSKKITVNVSGEILQLKETINTMVDQL
                     NAFAGEVTRVAREVGTEGRLGGQANVLGVAGTWKDLTESVNSMASNLTAQVRNIAEVS
                     TAIANGDLSKKITVNVSGEILQLKETINTMVDQLNSFAGEVTRVAREVGTEGRLGGQA
                     QVSGVAGTWKDLTDSVNSMASNLTGQVRNIAQVATAVARGDLSRTITVDVSGEILLLK
                     ETINTMVDQLNAFAGEVTRVAREVGTEGRLGGQAVVPGVAGTWKDLTDNVNFMANNLT
                     AQVRNIA"
     gene            complement(2379..2666)
                     /locus_tag="VFN42_04240"
     CDS             complement(2379..2666)
                     /locus_tag="VFN42_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HET8995854.1"
                     /translation="MESFFGGMITGGHLVAGLFFLKFWKRSQDALFAAFAMAFWLLAL
                     NQFLLVAMRVSSEEQSWIYLLRLAAFVLIAVAIVHKNTRRPAEPAGKFPER"
     gene            complement(2678..2941)
                     /locus_tag="VFN42_04245"
     CDS             complement(2678..2941)
                     /locus_tag="VFN42_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HET8995855.1"
                     /translation="MFESLVYVLCFLTSALCAWLLLASYRRRLQRLLLWSGLCFCLLA
                     LNSLLVFVDIVVLPTEIDLTSVRLATTLFALVVLLYGFVWEVD"
     gene            complement(2953..3318)
                     /locus_tag="VFN42_04250"
     CDS             complement(2953..3318)
                     /locus_tag="VFN42_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995856.1"
                     /translation="MSEDEILAFVRSAIGSVWTIELLVLLRQDPDRTWQARSLVTDLR
                     ASPRVVGDSLSALEAAGLVSADEGGLYRYQPVSRLIDDTARELVDLYARKPMTVVNTI
                     LSSPTNKIQTFADAFRFRK"
     gene            3610..4296
                     /locus_tag="VFN42_04255"
     CDS             3610..4296
                     /locus_tag="VFN42_04255"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HET8995857.1"
                     /translation="MRRRSLMLGALAASLPVAGSADADPVVLELFTSQGCSSCPPADA
                     LLGELERQPAVIALAWHVDYWNGLGWHDPFATRFATDRQRAYAARLRTEVYTPAVVVN
                     GGAVVVGSDRAAIAQAIARMPPPRVPIALRAGGSGVVASFDPALRPATVLLASYDPVR
                     DTAVGAGENTGRTLREYHVVRSVAVAPREAVAAGRVVFPPLAPGRGAVLLMHDASLRV
                     IGAAALAASG"
     gene            complement(4293..4754)
                     /locus_tag="VFN42_04260"
     CDS             complement(4293..4754)
                     /locus_tag="VFN42_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995858.1"
                     /translation="MARAKPIAGVAKRQHSLVWLQGLGCGAVVTLAPGFAALLGILLA
                     PGLVALLFDREAGRPMARCVLLTGMAACIAPVEMLWSNGQTVAAALGLLGDFQVMGTA
                     WSASAAGWLLAELAPLGSRLVLEALARSRAARLRAQRQVLIESWGFGDGGD"
     gene            complement(4754..>6547)
                     /locus_tag="VFN42_04265"
     CDS             complement(4754..>6547)
                     /locus_tag="VFN42_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995859.1"
                     /translation="ALQALIRPIDGLLSPLAHGRAPGPLNQPSYFVISRMPPGPPLAA
                     QPRPWPEAALLDQFLRPAALVLEQLQAVGVTHRAIRLNNLFQAAPGHPIVLGQAWAAP
                     PAMHQPAVHEPPYSGSCLPAGRGAGSIADDVYALGVTLLALALGRVPLAELDEAAVVR
                     RKLERGSHAALIGDERLPPVIADLVRGMLAEDPEHRPTPTLLLDPAAARGRRVAARPP
                     RHAQHGLTVAGVQAWDARTLANAVATEPDQGLHVLRNGTALQWLRREVGDAGLAARIE
                     ELLRHRLAESGPEETRSDALLTMRAVSLLDPLAPLCWRGLNLWPDGLGTALAAAQDTD
                     PASMARLEELVATEAISLWATARPDRCDFAMLRVEARQNRAWLQTGGLAGGLPRLLYG
                     LNPLVPCLSPLTASRWVARLNDVLPALEAAIAANPKLRPMDAHVAGFIAARTERGLDA
                     ELNMLAAPKPAVAEAAQLRLLAQIQAKLHREPLPALAAWLAEQTAPLLKQWHNRAKRA
                     ELEHGLRDLAQAGLLAPMLALVDDPAGRNADAQAAQVAANELVRIDAELSRLSAGTQQ
                     RAMQALRFGQELAAGIGLTVLATVLAIAVLS"
BASE COUNT         1001 a         2297 c         2216 g         1033 t
ORIGIN      
        1 ggcgatgtta cgcacctgcg ccgtcaggtt gttcgccatg aagttgacgt tgtcggtcag
       61 gtccttccac gttccggcca cgcccggcac gaccgcctgt ccacccaggc ggccctcggt
      121 gccgacctca cgcgccacgc gggtgacctc accggcgaag gcgttgagct gatccaccat
      181 cgtattgatg gtctctttga gcaagaggat ttcgccgctg acatccacgg taatcgtgcg
      241 cgagaggtcg ccgcgcgcca ccgcggtggc gacctgggcg atgttgcgca cctggcccgt
      301 caggttggag gccatggagt tgacgctgtc ggtcaggtcc ttccaggtgc cggcgacgcc
      361 ggacacctgc gcctgcccgc ccagccgccc ttcggtgccg acttcgcgcg ccacacgcgt
      421 cacctcgccg gcgaaggagt tgagctgatc gaccatcgtg ttgatggtct ccttcagctg
      481 caggatttcc cccgacacat tcacggtgat cttcttcgac aggtcgccat tggcgatcgc
      541 ggtggacact tcggcgatgt tgcgcacctg cgccgtcagg ttggacgcca tggagttcac
      601 cgactccgtc aggtccttcc acgtgccggc cacacccagc acgttggcct gtccgcccaa
      661 ccgtccttcg gtgccgactt cgcgcgccac gcgcgtcacc tcgccggcga aggcgttgag
      721 ctgatcgacc atcgtgttga tggtttcctt cagctgcagg atttcgccgg acacgttcac
      781 ggtgatcttc ttcgacaggt cgccctgcgc gatggcggtg gcgacctcgg cgatgttgcg
      841 cacctggccg gtcaggtttc cggccatgga gttcacgctg tcggtcaggt ccttccacgt
      901 gccggcgacg ccgggcacct gcgcctgacc gcccagccga ccttcggtgc ccacctcgcg
      961 cgcgacgcgg gtgacttcgg aggcgaagcc gttcaactgg tccaccatcg tgttgatggt
     1021 gtccttcagt tccaggattt cgccggacac gttcacggtg atcttgcggc tcaggtcgcc
     1081 gcgcgccacg gcggtggtca cctgggcgat gttgcgcacc tggtcggtca ggttgccgca
     1141 catggcgttg acgctgtcgg tcaggtcttt ccacgttccg gcaacaccgg gcaccagcgc
     1201 ctggccgcct agcttgccgt cggtgcccac ctcgcgcgcg acgcgcgtca cttccgacgc
     1261 aaaggaccgc agctggtcca ccatcgtgtt gatggcctcc ttgagctgca ggatttcgcc
     1321 gcgcacgtcc acggtgatct tcttcgacag gtcgccgttg gccaccgcga tcgtcacctc
     1381 ggcgatgttg cgcacctgcg cggtgaggtt ggacgccatg gagttcaccg actccgtcag
     1441 gtccttccac acgccggtca cctcgctgac ctgcgcctgg ccgcccagct tgccgtcggt
     1501 gcccacttcg cgcgccacgc gcgtgacttc cgaggtgaac acggacaact gcttgatcat
     1561 cgtgttgacg atcgtggcca gccgcaggaa ctcgccctgc agcggccgtc catccacgtc
     1621 cagccgcacg gtctgcagca ggtcgccctg cgccacggcg gcgatcgcct gcgtcaccgc
     1681 ggtggtcggc cacagcaggt catcgatcag cgtgttgacc gaggtttcca tctcccgcca
     1741 cgccccgtcg gacaagccga gcttgacgcg ggtgcgggtc ttgccttccc ggccgacgac
     1801 ctggcctacc tgttccagct cccgcgccat ccgctggttg gatgcgacga tgtcgttgaa
     1861 ggtgtcgcag atcttgcctt ccaggccggt gcagtcggtt ggcaggcgta ccgaaaagtc
     1921 gccggcccgc atggcctgca tcgcctgcag caggcgaggc aggtcgaagc cttcaccgcc
     1981 tgcggtcgcg gccgcacccg aggcggagcc atgggcgtcg gccggcacat gatcggcgtc
     2041 tgcaacaacg tctcgggtca ggtccatgat tctgctcggc tcaaggaaac aggggagtgc
     2101 ccgcagcggt cgtgtggcac gacagccgca gctgctaaca tccgcgggaa actagctggg
     2161 ctcggctgca cgcaccacag cggcatgcgg acaaaccagc gccacacaga aatgtgacag
     2221 gaaccggcgc cgggcggaac gtgaagtgcg ggaaacgaaa acccaggccc ctggcggtcc
     2281 agcctgcccg gtacgctatc gacatggccc gcgaagcata acgtctgcgt gaatccgccg
     2341 cgcggccagg cggcgcggaa ggcgctgtgt cggtatctct aacgttccgg gaacttgccg
     2401 gcaggctcgg ctggccgccg ggtattcttg tgcacgatcg cgaccgcgat cagcacgaag
     2461 gcggcaagcc gcagaagata gatccagctt tgctcctcgc tggacaccct catggcgacc
     2521 agcaggaact ggttcagcgc cagcagccag aacgccatgg cgaaggcggc aaacagcgcg
     2581 tcctggctgc gcttccagaa cttcaggaag aacagcccgg ccaccaggtg gccgccggtg
     2641 atcatgccgc caaagaagct ttccatctga cccaccgtta atccacttcc cagacaaagc
     2701 cgtagagaag caccaccagg gcgaacaacg tggttgccag acgaacggag gtcagatcga
     2761 tctcggtcgg tagcacgacg atgtcgacaa ataccagcag gctgttgagc gccagcagac
     2821 agaagcaaag cccgctccac agcagcagtc gttgcaggcg gcgccggtaa ctcgccagca
     2881 gcagccaggc acataaggcg ctggtcagga agcagagcac atacacaagg gactcgaaca
     2941 tctggcgatg gcctatttcc tgaagcggaa ggcatccgca aaggtttgga tcttgttggt
     3001 cggcgaggac aggatcgtat tcaccactgt cattggcttg cgggcataga ggtcgaccag
     3061 ttcgcgtgca gtatcgtcaa tcaggcggga cacaggctgg taacgataca atccgccctc
     3121 gtcggcactg acaaggcccg ccgcttccag ggcagacagg ctgtcgccca ccacgcgcgg
     3181 gctggcccga agatcggtga ccagcgatct cgcctgccag gtccggtccg gatcctgtcg
     3241 cagcaaaacc agcagttcga tcgtccagac cgagccgatg gcgctgcgaa cgaatgccag
     3301 aatttcgtcc tcgctcacca atcccgcctt gctgttgcgc gaacaggctg cggatggcac
     3361 tgcgaattgc ccccccgcca gccaacgacc ggacgcgcag ttcgcgtgct cgcactccga
     3421 tcgcctgaaa ccagtatcgc cgcgacccat cggctcgtgc aacccgggac gggaccccgc
     3481 cgcgcacggt tgcgcgttca gactattgcg aatgggcaaa atccgtgctg gccaatgctc
     3541 cgggcatctg tcgtgtaacg tctgcgctcg ccgccagtcc taccgatgca cgctggacag
     3601 gaggcacgca tgcggcggcg gagtctgatg cttggggcct tggcggctag ccttccggtg
     3661 gccggatcgg cggatgccga cccggtcgtg ctggagctgt tcacttccca gggctgttcc
     3721 tcctgcccgc ccgcggatgc cctgctcggc gagctggagc gtcagccggc ggtgatcgcc
     3781 ctggcctggc acgtggatta ctggaacggg ctgggctggc acgatccgtt tgccacgcgc
     3841 ttcgccaccg accggcagcg cgcctacgcg gcacgcctgc ggacggaggt ctatacgccg
     3901 gccgtggtgg tgaacggcgg ggcggtggtg gtcggctccg accgcgccgc catcgcccag
     3961 gcgatcgcgc ggatgccgcc gccgcgcgtg ccgatagcgc tgcgggccgg cggttcgggc
     4021 gtggtggcga gcttcgatcc ggcgctgcgg cccgcaacgg tgttgctggc cagctacgat
     4081 ccggtgcggg acacggcagt gggggcgggg gaaaacactg gccgcacact gcgcgaatac
     4141 cacgtcgtgc gctcggttgc cgtggcgccg cgggaagcgg tcgcagcggg gcgggtggtg
     4201 ttccccccct tggcgcccgg gcgaggcgcc gtgctgctga tgcatgacgc gtctctgcgc
     4261 gtgatcggcg ccgccgcctt ggccgcgtcg ggctaatcgc ccccgtcgcc gaagccccag
     4321 gactcgatca gcacctgccg ctgcgcgcgc aaccgcgcgg cgcgggagcg ggccagtgcc
     4381 tccagcacca gccggctgcc aagcggggcc aactccgcca gcagccaacc ggccgcggac
     4441 gcgctccagg cggttcccat cacctggaaa tcgcccagca ggcccagcgc cgcggcgacg
     4501 gtctggccgt tgctccacaa catttcgacc ggcgcaatgc aggccgccat gccggtcagc
     4561 aggacgcagc gcgccattgg ccgccccgcc tcgcggtcga acagcagcgc gaccagtccc
     4621 ggcgcgagca gtatgcccag cagcgcggcg aagcccggcg ccagggtcac cacggcgccg
     4681 cagcccagtc cctgcagcca gactagcgag tgctgccgct tcgcgacccc ggcgatcggc
     4741 ttggcgcgcg ccactagctg agcaccgcga tggccagcac ggtcgccagc acggtcagtc
     4801 cgatgccggc cgccagttcc tgaccgaaac gcagcgcctg catcgcgcgc tgctgggtgc
     4861 cggcggatag gcgggacagc tccgcatcaa tgcggaccag ctcattggcg gcgacctgcg
     4921 ccgcctgggc atccgcgttt cggccggccg ggtcgtccac cagggccagc atcggcgcca
     4981 gcagcccggc ctgcgccagg tcccgcaagc cgtgttccag ctcggcccgc ttggcgcggt
     5041 tgtgccactg cttcagcagc ggcgcggtct gttccgccag ccacgcggca agggccggca
     5101 gcggctcgcg gtgcaatttg gcctggatct gcgccagcag gcgaagctgc gccgcctcgg
     5161 ccacggcggg tttcggcgcc gccagcatgt tcagctccgc gtccagtccg cgctcggtgc
     5221 gggcggcgat gaagcccgcc acatgcgcgt ccatcggccg cagcttcgga ttggcggcga
     5281 tcgccgcctc cagcgccggc agcacgtcgt tcagccgtgc gacccagcgc gatgcggtca
     5341 gcgggctgag gcaggggacc agcggattca gtccatagag cagccgcggc aggccgccgg
     5401 ccaggccgcc ggtctgcagc caggcgcggt tctgccgcgc ctctacccgc agcatggcga
     5461 aatcgcagcg gtccgggcgc gcggtggccc agagcgaaat cgcctcggtc gcgaccagct
     5521 cctccagccg cgccatgctc gccggatcgg tgtcctgcgc ggcggcgagg gcggtgccca
     5581 gcccatccgg ccacaggttg agcccgcgcc agcacagcgg cgccagcggg tccaacagcg
     5641 ataccgcgcg catggtcagc agggcgtcgg accgggtttc ctccggaccg ctttccgcca
     5701 gccggtggcg cagcaattcc tcgatccggg cggcgaggcc ggcatcccct acctcccgcc
     5761 gcagccactg cagggcggtg ccgttgcgca gcacgtgcag cccctggtcc ggctcggtgg
     5821 cgacggcatt ggcgagggtg cgggcgtccc aggcctgcac gccggcaacg gtcagcccgt
     5881 gctgcgcgtg ccgcggcggc cgagcggcca cgcggcggcc gcgcgccgcg gcggggtcca
     5941 gcagcagggt cggggttggg cgatgctccg ggtcttccgc cagcatgccg cgcaccaggt
     6001 cggcgatcac cggcggcagg cgttcgtcgc cgatcagggc ggcgtggctg ccgcgctcga
     6061 gcttgcggcg gaccaccgcg gcctcgtcca gctcggctag cgggacgcgg cccagcgcca
     6121 aggcgaggag ggtgacgccc agcgcataga cgtcgtcggc gatgctgcct gcgccgcggc
     6181 cggccggcag gcaactgccg ctatagggtg gttcgtgcac cgctggctgg tgcatggcgg
     6241 gcggcgccgc ccaggcctgg ccgagcacga tgggatggcc gggcgccgcc tggaacaggt
     6301 tgttcagccg gatggcgcgg tgggtcaccc ccacggcctg caactgctcc agcaccaggg
     6361 cggccgggcg caggaactgg tccagcagcg ccgcctccgg ccagggccgt ggttgcgccg
     6421 cgagtggtgg tcccggcggc atccggctga tcacgaaata gctcggctgg ttgagcgggc
     6481 cgggcgcgcg gccatgcgcc agcggactga gcaggccgtc gatcggccgg atcagcgcct
     6541 gcaaggc
//
LOCUS       DASSZH010000199         4948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1300218, whole genome shotgun sequence.
ACCESSION   DASSZH010000199 DASSZH010000000
VERSION     DASSZH010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300218"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VFN42_04270"
     CDS             <1..213
                     /locus_tag="VFN42_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995860.1"
                     /translation="KPVIHEHLMEKLAPFIALHAAPSAEANELARGRELLSQRRRIEA
                     ETDNAKRRYVSGLAERTSPLFSSWRR"
     gene            complement(156..2648)
                     /locus_tag="VFN42_04275"
     CDS             complement(156..2648)
                     /locus_tag="VFN42_04275"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8995861.1"
                     /translation="MTWSAVQTRWSRFRNVSTALVLSAVLTFGLILIAGVILLRAHGS
                     LLREADKDALNVARAAGEDIARNLELYDLQLRTIARQMQNPGVQELQPDLRWAIMFDG
                     VARDEHLGFINVLNADGDVVADSASAAPRSGNFASRDYFQAQRHSVQDALFIGEPFDK
                     VHGAWSMIAISHRVSRPDGSFAGVVMGSVRLDYFAKLFNRFAIGRHGTITLLLANGMV
                     LQRSPAAPGEIGHIPTPNLLATGAGAAAFNAADPVDGRIRRFVLYRADGLPLFVAVGL
                     AQADIEAAWSGKARMIASALIMLGVLNAALLLWLWHAERKRNADAIQLRGQALTLEEY
                     ARQHARIVAEQKQAHERRLLALDDITHDVRTLLHSLLGHADLVQRKGPLNPKQMRHLA
                     SLRSTVEHLRHVADRFLRDARQGDGPALLLEPTDIRALIDNLGRITGLAAEEKGLSLN
                     LEVDGSLPPSIVTDGRRLSVILDNLLDNAIKCTGHGVVALHVSLESGALRCVISDTGP
                     GMPEAQRQRLLAGGDDFAASPGQARRGLSIIRRHIEALGGEASCLHNPGGGTIITFSV
                     PVTPALTPEPVEATSATRPMRILLVDDSAASREPTAELLRDAGHHITTAHSGEAAVQL
                     LRETGFDAVILDARMHGLSGPETARRIRAVPGSHGRVPIIVLSATPREQGLAAWQQAA
                     ISGYVEKSSDLSDLLRTIALATRPGTGCEPPLGPGSPPPACGAPNKDHLSGLAQDVLR
                     MRILLDGPGYAADRQALLELVHRLAGDTAQLGFTRLSTECRHYERAASPGSGTPPPAE
                     RLLRAANEALAQLHQRLQELNSGEVRSARPLT"
     gene            2828..3094
                     /locus_tag="VFN42_04280"
     CDS             2828..3094
                     /locus_tag="VFN42_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET8995862.1"
                     /translation="MRAARALLGIDQRQLAELCGLSLPTIQRMEASGGVVRGNVESLM
                     KLVTALNTAGIELIGEGVGSQTGGRGVRLMAPAESPADRSANGA"
     gene            3091..>4948
                     /gene="hyfB"
                     /locus_tag="VFN42_04285"
     CDS             3091..>4948
                     /gene="hyfB"
                     /locus_tag="VFN42_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HET8995863.1"
                     /translation="MIQTVLLGILGLLLLAPLGGLLARAPAGGPVVYGGSLLLSVLIL
                     CTGLAAIPAGTQHLVLPIGLPWTGAHIRLDALSGFFLGVVGLGSASASLFALGYGRHE
                     DEPQRVLPFYPLFLAGLNLVVVADDAFLFLFAWELMSLASWALVMAHHREQDNARAGY
                     VYLVMACFSGLTLLLGFGVLAGAGGSYAFDAMRSAHLSAGMAASVLLLALLGTGSKAG
                     LVPVHVWLPLAHPAAPSQVSALMSGVMTKVAVYGFIRIVFDLLGVPGWWAGVVVLVLG
                     GVTAVLGVLYALMEHDLKRLLAYHTVENIGIIFIGLGLAMAFRANGLQTAAALALTAA
                     LFHVFNHSVFKSLLFFGSGAVLTATGQRDMEKLGGLIHRMPRTAFAFLVGCAAISALP
                     PLNGFVSEWLTFQAILLHPNLPQWGLKVAIPADGALLALSAALAAACFVKAFGVSFLG
                     RPRSAAAAQAREVDRFSLAAMFVLAALCLLAGVFPGVVIDGLAPVAQALTGARMPSQL
                     GLQWLSIVPVAASRSSYDGLLLFVFIAVSGSLTAWAIHRFASRASRRGPAWDCGFPNA
                     DPAAQYTAGSFAQPIRRVFGSVVFRARDTETMPPPGDLSPARIQQVLRDPVWD"
BASE COUNT          728 a         1629 c         1681 g          910 t
ORIGIN      
        1 aagccagtga tccacgagca cctgatggag aagctggccc ccttcatcgc cttgcacgcc
       61 gcgccctcag ccgaggcgaa tgaactggcg cgcgggcggg aactgctgag ccagcgccgc
      121 cgcatagaag cggaaaccga caacgccaag cgtcgctacg tcagcggcct ggccgagcgc
      181 acctcgccac tgttcagctc ctggaggcgc tgatgcaact gtgccaacgc ctcgttcgcc
      241 gccctgagca accgctcggc cggtggcggt gtgccacttc ccggggacgc cgctcgttca
      301 tagtgcctgc attccgtcga caagcgggtg aaccccagtt gagcggtgtc gccggccagg
      361 cggtgtacca gttccagcag tgcctgccga tccgccgcat atcccggtcc atcgagcagg
      421 atgcgcatcc gcagcacgtc ctgggcaagt ccgctgaggt gatccttgtt cggtgcgccg
      481 caggcgggcg gaggtgagcc cgggcccagc ggcggttcgc acccggttcc tggccgcgtc
      541 gccagggcga tcgttcgcag caggtccgac aggtcgctgg atttctccac gtagccgctg
      601 attgctgcct gttgccatgc cgcgagaccc tgctctcgcg gcgtcgccga caggacgatg
      661 atcggcaccc ggccatggct tcccggaacc gcccggatcc gtctggcggt ttccggaccc
      721 gacaatccgt gcatgcgtgc gtcgaggatg acggcatcga acccggtctc acgtagaagc
      781 tgcacggcgg cctctcccga gtgtgccgtg gtgatatggt gtccggcgtc gcgcagcaat
      841 tccgccgtcg gttcgcggct cgcggccgaa tcgtccacca gcagtatccg cattggccgg
      901 gtagcggacg tcgcttccac aggttccggc gtgagggccg gcgtgacggg cacgctgaag
      961 gtgatgatgg tgccgccccc tggattgtgc aggcagcttg cctcgccacc gagcgcttcg
     1021 atgtggcgcc tgatgatcga caggcctcgg cgtgcctggc cgggggaagc ggcaaagtcg
     1081 tcgccgcccg cgagcagccg ttgtcgttgg gcctccggca tgccggggcc cgtgtcgctg
     1141 atcacgcagc gcagtgcgcc ggattccagc gagacgtgca gcgccaccac gccgtgtccg
     1201 gtgcatttga tcgcattgtc gagcaggtta tccaggatca cgctcagccg ccggccatcc
     1261 gtgacaatgc ttggcggcag gctgccatcg acttccaggt tcaggctcag ccccttttcc
     1321 tctgccgcca ggcctgtaat gcggccaaga ttgtcgatca gtgcgcggat atcagttggt
     1381 tccagcagca gtgccggccc gtctccctgg cgggcgtcgc gcaggaaccg gtctgccaca
     1441 tgccgcaggt gctccacggt gctacgtaag gatgccaggt ggcgcatctg ctttgggttc
     1501 agcgggcctt ttcgctgcac cagatcggcg tggcccagca gactgtgcaa cagggtacgc
     1561 acatcatggg tgatatcatc gagcgccagt aaccggcgtt catgcgcctg cttctgttcg
     1621 gcgacgatgc gggcatgctg tctcgcgtac tcctccagcg tcagcgcctg cccgcggagc
     1681 tgtatggcgt ccgcgttccg cttgcgctct gcatgccaca gccagagcag cagtgccgcg
     1741 ttcagcactc ccagcatgat caaagcggag gcgatcatgc gcgcctttcc gctccacgcc
     1801 gcctcaatgt cggcttgcgc cagtccgaca gcgacgaaga gcggcaatcc gtccgcccga
     1861 tacagcacga agcgccggat ccgtccgtcc accgggtcag cggcgttgaa agctgcggca
     1921 ccagcgccgg tcgccagcag gttcggcgtc gggatgtggc cgatctcgcc aggcgccgcc
     1981 ggactccgct gcagaaccat gccattggcc agcagcagcg tgatcgtgcc atgccggccg
     2041 atggcgaacc gattaaacag cttggcaaaa taatccagcc gcacgcttcc catcaccacg
     2101 ccggcgaagc tgccgtcggg ccgcgatacg cgatggctga tcgcgatcat gctccacgcg
     2161 ccatgcactt tgtcgaaggg ttcgccgatg aacagcgcat cctggacgct atgacgttgc
     2221 gcctggaaat agtcgcggct ggcgaagttg ccgctgcgag gcgcggcgga ggccgagtcc
     2281 gcaaccacat cgccatccgc gttcagcacg ttgatgaacc cgagatgttc atcgcgcgcc
     2341 acgccatcga acatgatcgc ccagcgcaaa tccggctgca gctcctgcac gccgggattc
     2401 tgcatctgtc gcgcaatcgt ccgcagctgc agatcgtaca gctccaggtt gcgcgcgatg
     2461 tcctccccgg cggcgcgcgc gacgttcagc gcgtccttgt cggcctcccg caggagactg
     2521 ccgtgcgccc gcagcagaat gaccccggca atcaggatta gcccgaacgt gagtaccgcg
     2581 ctgagcacga gtgcggtgct gacgtttcgg aaccgcgacc agcgagtctg aacggcggac
     2641 caggtcatcg ggttttcccg gcggaatgcc ggaagggtag ggaacaatcg ctaggaaaag
     2701 gttgacgcca gcgccggcca gaattgcaga aaatcaagct tgaattctta gttctgatgt
     2761 gcaatgcatc actactgcca gataaacacc aatctctcac gttcgaagca tgataaccgc
     2821 aggtcagatg cgcgccgccc gggcgctgct ggggatcgac cagcgccagt tggccgagct
     2881 ctgcggcctg tcgctgccga ccatccaacg catggaggcc agcggcggcg tggtccgcgg
     2941 caatgtcgag tccctgatga agctggtgac cgccctgaac accgccggca tcgagctgat
     3001 cggcgaaggc gtcggcagcc agaccggcgg ccgtggcgtc cgtctgatgg cgcccgccga
     3061 atcgccggcc gatcggagcg ccaacggcgc atgatccaaa cggtgttgct cgggatactg
     3121 ggcttgctgc tgctggcacc gctcggcgga ttgttggccc gggctccggc cggcggtccc
     3181 gtggtctatg gcggcagtct gctgttgtcg gtgctgatcc tgtgcaccgg cctcgccgcc
     3241 attccggcgg ggacgcagca tctcgtgctg ccgatcgggc tgccctggac cggcgcgcat
     3301 attcgtctgg acgcgctgtc cgggttcttc ctcggcgtgg tcggattggg cagcgcttcg
     3361 gccagcctgt tcgcgctggg ctacgggcgg catgaggacg aaccgcaacg tgtactgccg
     3421 ttctacccgc tgttcctcgc cgggctgaac ctggtggtgg tcgcggacga cgccttcctg
     3481 ttcctgttcg cctgggagct gatgtcgctc gcctcctggg cgctggtgat ggcgcatcat
     3541 cgcgaacagg acaacgcgcg tgccggctat gtctacctgg tgatggcctg cttctccggt
     3601 ctgacgctgc tgctgggctt cggcgtgctg gccggggcag gaggcagcta cgcgttcgat
     3661 gcgatgcgtt cggcacacct gtccgctggc atggccgcgt ccgtactgct gctggctctg
     3721 cttggcaccg gatcgaaagc cggtttggtg ccggtgcatg tgtggctgcc gctcgcccat
     3781 cccgcggcgc cgagccaggt atcggcgctg atgagcgggg tgatgacgaa ggtcgcggtg
     3841 tacggcttca tccgcatcgt gttcgacctg ctcggcgtgc cgggctggtg ggccggcgtg
     3901 gtggtgctgg tgctgggtgg tgtcactgcg gtgctgggtg tgctgtacgc gctgatggag
     3961 cacgatctga agcggctgct cgcgtaccat acggtagaaa atatcggcat catcttcatc
     4021 ggcctcggcc tggcgatggc attccgcgcc aacggcctgc agacggccgc cgcactggcg
     4081 ctgaccgcgg cgctgttcca cgtattcaac cactcggtgt tcaagagcct gctgttcttc
     4141 ggttccggcg cggtgctgac cgcgaccggg cagcgcgaca tggagaagct gggggggctg
     4201 atccaccgca tgccgcgaac cgccttcgcg ttcctggtgg gctgcgcggc gatctccgcg
     4261 ctgccgccgc tgaacggctt cgtgtcggag tggctgacct tccaggcgat cctgctgcat
     4321 ccgaacctgc cgcaatgggg gctgaaggtc gcgatcccgg cggacggcgc gctgctggcg
     4381 ctgtcggcgg cgctggcggc cgcgtgcttc gtgaaggcgt tcggggtgag tttcctgggc
     4441 cggccacgca gtgccgcggc ggcgcaagcc cgcgaggtgg atcgcttctc gctcgccgcg
     4501 atgttcgtgc tggccgcgct gtgtttgctg gcgggggtgt tccccggggt ggtgatcgac
     4561 gggctggcac cggtcgcgca ggcgctgacc ggcgcgcgca tgccgtcgca gctcggcttg
     4621 caatggctgt cgatcgtgcc ggtggcggcg agccgcagtt cgtatgacgg gctgctgctg
     4681 ttcgtgttca tcgcggtgtc cgggtcgctg acggcgtggg ccatccaccg gtttgcgtca
     4741 cgcgcgtcgc ggcgcgggcc ggcctgggat tgcgggttcc cgaacgccga tccggcggcg
     4801 caatacaccg ccggcagctt cgcgcagcca atccgccgcg tgttcggcag cgtggtgttc
     4861 cgggctcgcg acacggagac gatgccgcca cccggcgacc tgtcgccggc ccgcatccag
     4921 caggtgctgc gcgatcccgt atgggaca
//
LOCUS       DASSZH010000200         4343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_839692, whole genome shotgun sequence.
ACCESSION   DASSZH010000200 DASSZH010000000
VERSION     DASSZH010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839692"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1116)
                     /locus_tag="VFN42_04290"
     CDS             complement(34..1116)
                     /locus_tag="VFN42_04290"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HET8995864.1"
                     /translation="MSVVLRAAAPVPARVMVCDDSLVIRAAISRMLQTDPGIEVVARV
                     ANGQAALDALKQSKPDVVVLDIEMPVMDGMTALPLLLRADPGLRVVMASTLTTRGADI
                     ALRALRLGAADYLPKPSSIGSVSDDGFRRELLEKVRGLARLRHRAVEPPSTGAPARLT
                     LRPAPAMPARLLAVGSSTGGPQALFTLVQGLGRALAVPVVLTQHMPPTFTPILAEHLT
                     KLGGMPCGEAKDKEVLLPSRIYLAPGDRHLLVEGARGAMRARLSDAPAENFCRPSVDP
                     MLRSAAAACEGRVLVAMLTGMGHDGLAGTRVVVQAGGAALAQDETTSVVWGMPGAIAE
                     AGLCHAVLPLTRLAPKLLDMLRGARL"
     gene            complement(1113..1481)
                     /locus_tag="VFN42_04295"
     CDS             complement(1113..1481)
                     /locus_tag="VFN42_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8995865.1"
                     /translation="MPRTCLVVDDSRVVRKVARRILEANGYTVAEAPDGAQALALCRQ
                     ALPDCVLLDWNMPVMDGMSFLHALRAEFGPADPPVVFCTTENDMAHIAQAIEGGAQEY
                     IMKPFDEEILVSKLAQVGLT"
     gene            complement(1491..1964)
                     /locus_tag="VFN42_04300"
     CDS             complement(1491..1964)
                     /locus_tag="VFN42_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET8995866.1"
                     /translation="MTLDVLDRADAAAEGEQVFVTLTLADQLCGVPVQGVRDILSEQA
                     ITRIPLAPPEIAGSLNLRGRIVTAIDLRRRLKLPPPQAGQPRMSVVAEQGGELYALLV
                     DQVSEVMTLRADAFERNPPTLPPEWAAYSQGIFRLDGKLLVVLDVGRLLAVGKAD"
     gene            complement(1983..2600)
                     /locus_tag="VFN42_04305"
     CDS             complement(1983..2600)
                     /locus_tag="VFN42_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995867.1"
                     /translation="MEVEQQTDAQATHSKVGQNLRLMRRNDAGHSLDLNNNLPFHENV
                     SAESFIKSDILIEDRNCDLPCEGDPSCLKFMAQATFIHGFQHPAACHPMYLDCQSDYP
                     LSQFARNQHPNSPSRSVFLSALRVKGLTLTNLETPGFWRGDRQQFYRFSDTAIRQVLP
                     EVSVAFLRVSGHLPADFASTWDPAGLGPFKQMAAEQVEDHRVHSG"
     gene            complement(2813..>4343)
                     /locus_tag="VFN42_04310"
     CDS             complement(2813..>4343)
                     /locus_tag="VFN42_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET8995868.1"
                     /translation="RGFATEAELAAMSEQQIYRFIFRPGFSTAAQVTSVSGRGVGMDV
                     VKTNIERIGGSIDLRSKPGEGTCFTITIPLTLAIVSALIVEAAGERFAIPQASVVELV
                     HAAPGRQEEGAARIDRIDGTLILRLRDRLLPLISLAELLRLPGATCDASAGAHIVVAS
                     VGTATLGLIVDRVFDTEEIVVKPVAPILRHITMFSGNTILGDGSVIMILDPNGIARGT
                     GIGAGGDSKAMSSSAMDALHSTDRTAMLLFRAGDQQAMAVPLGLVARLEDIPRDRIEI
                     SCGAPVTQYRGKLMPLVALSGGFDAERPHHPVLVFADRDRSMGLMVDEIVDVVEDRLE
                     IELSGARPGLLGTAVIAGHATDVIDTGYWLTQAWHDWFRNLPQGGTAGGPRRVLVVED
                     SDFFRQLLVPTLGAAGYRVTAAPSAAEALRLRESGMMFDAIVSDIEMPEMDGLAFAHA
                     VRESGSWAALPMIALSGHADPRDVEAGRDAGFTDYVAKFQRDALLSSLQQCLSEPVTV
                     G"
BASE COUNT          724 a         1498 c         1426 g          695 t
ORIGIN      
        1 gggaggggga atactgcagg gctaccaccc ccttcacagc cgtgccccac gcagcatgtc
       61 gagtagcttc ggcgccaacc gggtcagcgg caggacggcg tggcacagac cggcctcggc
      121 gattgcgccg ggcatgcccc agaccacgct ggtggtctcg tcctgcgcca gcgccgcgcc
      181 cccggcctgg accaccaccc gggtgccggc cagcccgtcg tgacccatgc cggtcagcat
      241 cgccaccagc acgcggccct cgcaggcggc ggcggcgctg cgcagcatcg gatcgaccga
      301 aggccggcag aaattctccg ccggtgcgtc ggacagccgc gcgcgcatgg cgccgcgcgc
      361 gccttccacc agcaaatgcc ggtcgcccgg cgccaggtag atccggctcg gcagcagcac
      421 ctccttgtcc ttcgcctcac cacacggcat gccgccgagc ttcgtgaggt gttcggcgag
      481 gatcggggtg aaggtgggcg gcatgtgctg ggtcagtacc accggcacgg caagcgcccg
      541 accgagcccc tgcaccaggg tgaacagcgc ctgcggcccg ccggttgagc tgcccaccgc
      601 cagcaagcga gccggcatgg ccggcgccgg acgcaaggtc agccgcgccg gggcgccggt
      661 ggacgggggt tccaccgcgc ggtggcgcag ccgggccagc cccctcacct tctccagcag
      721 ctcacggcgg aaaccgtcat ccgagacgga cccgatcgag gacggtttcg gcaggtagtc
      781 cgccgcaccc agccgcaacg ctcgcagcgc gatgtcggcc ccccgcgtcg tcagcgtgga
      841 cgccatcacc acacgcagcc cggggtcggc ccgcagcagc agcggcagcg cggtcatgcc
      901 gtccatcacc ggcatctcga tgtccagcac caccacgtcc ggcttgctct gcttcagcgc
      961 gtccagcgcc gcctggccat tggccacgcg cgccacgacc tcgatgcccg gatcggtctg
     1021 cagcatgcgg ctgatggcgg cgcggatcac cagggagtca tcgcatacca tcacccgtgc
     1081 cggtaccggc gcggcggcgc gcagcacgac gctcatgtca gcccgacctg ggccagcttg
     1141 ctgaccagga tctcctcgtc gaacggcttc atgatgtatt cctgcgcgcc gccctcgatc
     1201 gcctgggcaa tatgcgccat atcgttctcg gtagtgcaga acaccactgg cggatcggcc
     1261 ggaccgaact cggcgcggag ggcatgcagg aaactcatgc cgtccatcac cggcatgttc
     1321 caatccagca gcacgcagtc gggcagcgcc tggcggcaca acgccagggc ctgcgcgcca
     1381 tcgggcgctt cggcaaccgt gtagccattc gcttccagaa tccgccgagc gaccttccgc
     1441 accacacggc tgtcatccac gaccaggcag gttcgcggca tgcggcggcc tcaatccgct
     1501 ttgcccacgg cgagcaaccg gcccacgtcc agcaccacga gcagcttgcc gtcgaggcgg
     1561 aagatcccct ggctgtaggc cgcccattcc ggcggcaggg tcggcggatt gcgctcgaac
     1621 gcatcggcac gaagcgtcat cacttccgag acctggtcca ccagcagcgc gtagagttct
     1681 cccccctgct ccgccaccac ggacatgcgc ggctggcccg cttgcggcgg cggcagcttg
     1741 aggcggcggc gcaggtcgat cgcggtgacg atgcggccgc gcaggttgag gcttccggcg
     1801 atctccggcg gcgccaaggg gatgcgggtg atcgcctgct cgctgaggat gtcgcgcacg
     1861 ccctgcaccg gaacaccgca aagctggtct gcaagggtca gcgtgacgaa cacctgctca
     1921 ccctcggcgg cggcatcggc acggtccagg acgtcgagcg tcatgcgaag tctcctgctt
     1981 ggtcagccgc tgtggacgcg gtggtcctct acctgttccg cagccatctg cttgaaggga
     2041 ccgaggccag ccggatccca ggtgctggcg aaatccgcgg gcaggtggcc gctcacacgc
     2101 aggaatgcca ccgagacttc gggaagcacc tgacggatgg ccgtgtcgct gaaccggtag
     2161 aactgctgtc tatcgccgcg ccagaagccc ggggtttcaa ggttcgttaa ggttaggcct
     2221 ttaacacgga gggcagaaag aaacacggag cgggacggag aattcggatg ctgattgcgc
     2281 gcgaattgac tgagcgggta atcggactgg caatcgaggt acatcggatg acaggccgcg
     2341 ggatgctgga atccgtgtat gaaggttgct tgtgccatga acttgaggca gctgggatca
     2401 ccttcgcacg gcaggtcgca attccgatct tctataagaa tatcagattt gatgaaggat
     2461 tccgcgctga cattctcgtg gaacggcaaa ttattattga ggtcaaggct gtgtccagca
     2521 tcgttcctgc gcatgaggcg caggttctga cctaccttcg aatgagtggc ttgcgcgtcg
     2581 gtctgctgtt caacttccat tcccggttgc tgaaggatga tatgcgacgt ttggtcgtct
     2641 gaatctctcc gtgccgctcc gtggttcttt ctgatctccg tgtttattgc ttgccacagc
     2701 agattcacta ccgacgctgc agcgtgacca ctcctgagta ccggccggga cgcctttctg
     2761 ggcttctcca cctccggcac gccgtcctga atcgaactct gcctggcttc cctcatccca
     2821 cagtcaccgg ttcgctcagg cactgctgca ggctggacag caatgcgtcg cgttggaact
     2881 tcgccacgta atcggtgaac ccggcgtcgc gcccggcttc cacgtcgcgc ggatcggcat
     2941 ggcctgacag ggcaatcatc ggcagcgccg cccaggatcc ggactcgcgc acagcatggg
     3001 cgaaggccag cccgtccatc tccggcatct cgatatccga cacgatggcg tcgaacatca
     3061 tgccggactc gcgcaaccgc agcgcctccg cggcactcgg cgccgccgtc acgcggtagc
     3121 ccgcggcacc cagcgtgggc accagcaatt ggcggaagaa gtcgctgtcc tccaccacca
     3181 ggacgcggcg gggaccgccg gccgtcccgc cctgcggcag attgcggaac cagtcgtgcc
     3241 aagcctgggt caaccagtag ccggtgtcga tcacgtcggt cgcatgcccg gcgatcaccg
     3301 cggtgcccag caggccggga cgcgcgccgg agagttcgat ctccagccgg tcctccacca
     3361 cgtcgacgat ctcgtccacc atcaggccca tgctgcggtc gcggtcggcg aacaccagca
     3421 ccggatgatg cggccgctct gcatcgaacc cgccggacag cgccaccagc ggcatcagct
     3481 tgccgcggta ttgggtgacc ggcgcgccgc aagagatctc gatcctgtcg cgcgggatgt
     3541 cttccagccg cgcgaccagt cccaagggta cggccatggc ctgctggtcg ccggcgcgga
     3601 acagcaacat cgcggtgcga tcggtggagt gaagcgcatc catcgcgctc gacgacatcg
     3661 ccttgctgtc gccgccagca ccaattccgg tgccgcgcgc gatgccgttc gggtcgagga
     3721 tcatgatgac gctgccgtcg cccaggatcg tgttgccgct gaacatcgtg atgtggcgca
     3781 ggatcggcgc cacgggcttc accacgatct cttccgtgtc gaagacgcgg tccacgatga
     3841 ggccgagggt cgcggtcccg accgaggcga cgacgatgtg ggcgcccgcg gacgcgtcgc
     3901 aggttgcgcc ggggagccgg agcagctcgg cgagcgagat cagcggcagc agccggtcgc
     3961 gtaaccgcag gatcagcgtg ccgtcgatcc ggtcgatccg tgccgcgccc tcctcttggc
     4021 ggccgggggc tgcgtggacg agttcgacga cgctcgcctg cgggatcgcg aagcgctccc
     4081 ccgcggcctc gacgatcaag gccgagacga tggccagcgt gagcgggatg gtgatggtga
     4141 agcaggtgcc ctcgccgggc ttgctgcgca ggtcgatcga gccgccgatc cgctcgatat
     4201 tcgtcttgac cacgtccatg ccgacgccgc ggcccgagac cgaggtcacc tgggcggcgg
     4261 tcgagaaacc ggggcggaag atgaagcggt agatctgctg ctcgctcatg gcggcgagct
     4321 cggcctcggt cgcgaagccg cgg
//
LOCUS       DASSZH010000201         4824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1351072, whole genome shotgun sequence.
ACCESSION   DASSZH010000201 DASSZH010000000
VERSION     DASSZH010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351072"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1642)
                     /locus_tag="VFN42_04315"
     CDS             complement(<1..1642)
                     /locus_tag="VFN42_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol/ethanol family PQQ-dependent
                     dehydrogenase"
                     /protein_id="HET8995869.1"
                     /translation="MKKTSFKSLLAAGVFLSAALVAGAAMAQDDLAKLQENPANWASP
                     GRTDDMQRFSTLDQINTGNVGKLQVAWTFSTGVLRGHEGQPLVIGDVMYVHTPFPNIV
                     YALDLNNDGKILWKYEPKQDPSVVPVMCCDTVNRGVAYGDGKIFLHQADTTLVALDAT
                     TGKVAWSVKTDDPAKGATGTDAPFVFKDKVYVGVSGGEFGVRGWVAAYSVKDGKQVWR
                     AYSMGPDSDVLIDAQKTTDLGKPVGADSSLKTWKGDQWKIGGGATWGWYGYDPKLNLI
                     YYGTGNPSTWNPSQRPGDNKWSMTIFARDADTGMAKWVYQMTPHDEWDYDGVNEMILA
                     DQEIGGHKVPTLVHFDRNGFGYTLNRETGELLVAAKFDPAVNWATGINMDKSSKDYGR
                     PIRDPKYTTEEADVNHTGICPAALGTKDEQPAAYSPETGLFYVPTNHVCMDYQPFRVS
                     YTAGQPYVGATLSMYPPKGDSHMGNFIAWDATKGKIVWSNPEMFSAWGGALATKGNVV
                     FYGTLEGYLKAVDAKTGKELYSFKTPSGIIGNVISYEHG"
     gene            complement(2276..2629)
                     /locus_tag="VFN42_04320"
     CDS             complement(2276..2629)
                     /locus_tag="VFN42_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995870.1"
                     /translation="MARPVVLGIALALCALPLWPALAGPSTNDYPTEARADYVFACMA
                     TNGGTREALQRCSCVIDVIAGLLPYGDYEKAETVLRMRHLTGGYLAQTFHVPQANVFV
                     ERLQDAQAEGDVRCF"
     gene            complement(2644..3729)
                     /gene="pqqE"
                     /locus_tag="VFN42_04325"
     CDS             complement(2644..3729)
                     /gene="pqqE"
                     /locus_tag="VFN42_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HET8995871.1"
                     /translation="MQRPDPPMGMLLELTHRCPLQCPYCSNPLRLERAGDELPTETWL
                     SVIDQAAELGILQAHFSGGEPMARRDLVTLVRHAAARGLYGNLITSAVLLDAESLQTL
                     AEAGLDHVQISLQDVVPEGADRIANYAGAHAKKLAAARLVREAGLALTLNFVVHRHNI
                     ARVPEMIALGEAMGAGRIEIANVQYYGWGLRNRAALLPTRAELDAATEAVFAGRARLA
                     GRMVIDYVIPDYYAAEPKGCMGGWGRRFLNVSPAGRVLPCHAAETLPGFEWPSVRTAT
                     LAEIWYGAEAFARYRGTAWMPEPCRSCDRRDIDWGGCRCQAFALTGDAGRTDPACARS
                     PDHAVMAAAAREAEAPAPAFVYRRIGG"
     gene            complement(3837..3968)
                     /gene="pqqA"
                     /locus_tag="VFN42_04330"
     CDS             complement(3837..3968)
                     /gene="pqqA"
                     /locus_tag="VFN42_04330"
                     /inference="COORDINATES: protein motif:HMM:NF001400.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HET8995872.1"
                     /translation="MRTGWAPHRPHNPEDRIMAWTTPKIVEVALGGEINCYACARAK"
     gene            complement(4037..>4824)
                     /locus_tag="VFN42_04335"
     CDS             complement(4037..>4824)
                     /locus_tag="VFN42_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HET8995873.1"
                     /translation="HSRVDATIDRIVERARVGNIYVNRNMIGAVVGVQPFGGHGLSGT
                     GPKAGGPLILRRLLAAAPPALPLAKPAEPPHAAAARTWIEFARPFTTEAAIRACERAL
                     AQSAVGATVDLPGPVGERNVYALEPRGRVLCLAATPEGMVAQVGAALAGGNSAIVLPP
                     HNPNDFLPRLPPALRGRVQRVDDVRGVACEAVLYDGDAESLRHWLGVLAAGTGPVVPV
                     LAPPAGSVLEGSADYDLAVLVGERAVSTNTAAAGGNASLMSVG"
BASE COUNT          787 a         1703 c         1527 g          807 t
ORIGIN      
        1 cgccatgctc gtaggagatg acgttgccga tgattccgga cggcgtcttg aagctgtaga
       61 gttccttgcc ggtcttggca tcgaccgcct tcagatagcc ctccagcgtg ccatagaaca
      121 ccacgttgcc cttggtcgcc agcgcgccgc cccaggccga gaacatctcc gggttcgacc
      181 agacgatctt acccttggtg gcgtcccagg caatgaagtt gcccatgtgg ctatcgccct
      241 tcggcggata catggacagg gtcgcaccga catagggctg acccgccgtg taggatacgc
      301 ggaacggctg gtagtccatg cagacatggt tcgtgggaac gtagaacagg ccggtctcag
      361 gcgaatacgc cgccggctgt tcatccttgg tgccgagcgc ggccgggcag atccccgtat
      421 ggttcacgtc cgcttcttcc gtcgtgtatt tcggatcgcg gatcggcctg ccgtagtcct
      481 tgctcgactt gtccatgttg atcccggtcg cccagttcac cgcgggatcg aacttggctg
      541 ccacgagcaa ctcgcccgtc tcacggttca gcgtatagcc gaaaccgttg cggtcgaagt
      601 gaaccagcgt cggcacctta tggccaccga tctcctggtc ggccaggatc atctcgttga
      661 cgccgtcgta gtcccactcg tcgtgcggcg tcatctgata gacccacttc gccatgccgg
      721 tgtcggcgtc acgcgcgaag atggtcatcg accacttgtt gtcgccgggg cgctggctcg
      781 gattccaggt ggaagggttg cccgtcccgt aatagatcag attgagcttc ggatcgtagc
      841 cgtaccagcc ccaggtcgcg ccgccgccga tcttccactg gtcacccttc caggtcttca
      901 gcgaggagtc cgcgccgacc ggcttgccga ggtcagtggt tttctgcgcg tcgatcagca
      961 cgtcgctgtc cgggcccatc gaataggcgc gccagacctg cttgccgtcc ttgaccgaat
     1021 aagcggcaac ccagccgcgc acaccgaatt cgccgccgga gacgccgaca tacaccttgt
     1081 ccttgaagac gaacggcgca tcggtgcccg tggcaccctt ggcgggatca tcggtcttca
     1141 ccgaccacgc gaccttgccg gtcgtggcgt cgagtgcgac cagcgtggtg tcggcctggt
     1201 gcaggaagat cttgccatcg ccataggcga cgccgcgatt caccgtgtcg cagcacatga
     1261 ccgggacgac gctcggatcc tgcttgggct cgtatttcca gaggatcttg ccgtcattgt
     1321 tcaggtcgag cgcatagacg atgttgggga acggcgtgtg cacgtacatc acgtcgccga
     1381 tcaccagcgg ctggccctcg tggccgcgca gcacgccggt ggagaatgtc cacgcgacct
     1441 gaagcttccc gacattgccg gtgttgatct gatccagcgt cgagaaccgc tgcatgtcat
     1501 cggtgcgtcc cggcgacgcc cagttggccg ggttctcctg cagtttcgcc agatcatcct
     1561 gtgccatcgc ggcgcccgcg accagcgcag ccgacaagaa cacgcctgcc gcaagcaggc
     1621 tcttgaacga ggtcttcttc atgtatcctc ccgttctgcc gagacggcag cacgttgtta
     1681 tgacactggc cgcccggctt atgccgaccg gccgccgttc gctcgggatg ctcaaccttc
     1741 ggcagcgtgt cacgacggcc gcaacggcac atcttcgagg cgtgcccgcc ttcccaaaag
     1801 cggacggcct tgaagccgaa gtgcgcgaag gctacggcgc gcgcccgaca tccgcaagcc
     1861 gtacgttgat aacgcacgag cgaacttccc cggcacaagc atgaaaccta acatcgcaga
     1921 tagttgaaca acattgcgac ggctccatgc attgcccatc cccttcattt atatacatgt
     1981 tcttcatttg gagattaaga cgaaattgca actaaaccgc tttcggacgt tataaattta
     2041 tctattttgt catttttgga atgcgcgttt catggcagcc gcccgtgacg ctgccccttc
     2101 ttcgtcattc ccgcgcaaac gggactccac ggcgagcgcc tgggatggca cgcgactccg
     2161 ctgaaagccc acacaggcgg gcattccggg ggatgcgctg cggaattccg tgcggaacct
     2221 cagaattccc gccggcaccg gaatgacaaa aaagtcagag tggcggccac tgggatcaga
     2281 agcagcgaac gtcgccctcg gcctgcgcgt cctgcaaccg ttccacgaac acattcgctt
     2341 gcgggacgtg gaatgtctgc gccagatagc cgccggtcag atggcgcatg cgcagcaccg
     2401 tctccgcctt ctcgtagtcg ccatacggca acaggccggc gatcacgtcg atgacgcagg
     2461 aacagcgctg cagcgcctcg cgcgtgccgc cgttcgtcgc catgcaggcg aatacgtagt
     2521 cggcgcgcgc ctccgtcgga tagtcattcg tcgacggccc cgccagggcc ggccagagcg
     2581 gcagggcgca aagcgccaag gcgatcccca gcacgaccgg acgtgccatc gtccccctcc
     2641 ggatcagccg ccgatgcggc gatacacgaa cgccggtgcc ggcgcttcgg cctcgcgggc
     2701 cgccgccgcc atcaccgcgt gatcgggtga acgcgcacag gccgggtcgg tgcgccccgc
     2761 atcgccggtc agcgcaaacg cctggcaccg gcaaccgccc cagtcgatgt cgcgccggtc
     2821 gcaggaccgg cagggttccg gcatccaggc cgtgccgcga tagcgcgcga acgcctccgc
     2881 cccgtaccag atttccgcga gcgtcgccgt ccgcaccgac ggccactcga accccggcag
     2941 cgtctccgcg gcatggcacg gcagcacgcg gccggccggg gagacattca ggaaccgccg
     3001 cccccagccg cccatgcagc ccttcggctc ggcggcgtag tagtcgggga tcacgtagtc
     3061 gatcaccatg cgcccggcca gccgcgcgcg cccggcgaac acggcctcgg tcgcggcgtc
     3121 cagctcggcg cgcgtcggca gcagggcggc gcggttgcgc agcccccagc catagtattg
     3181 cacgttggcg atctcgatgc gcccggcgcc catcgcctcg cccagcgcga tcatctccgg
     3241 cacgcgcgcg atgttgtggc ggtggacgac gaaattcagc gtcagcgcca gccccgcctc
     3301 ccgcaccagc cgcgccgccg ccagcttctt cgcatgcgcg ccggcatagt tggcgatgcg
     3361 gtcggcgccc tccggcacca cgtcctgcag gctgatctgc acgtgatcga gcccggcctc
     3421 cgccagcgtc tgcagcgact ccgcgtcgag cagcaccgcc gaggtgatca ggttgccata
     3481 caggccgcgc gcggcggcgt gacgcaccag cgtgaccaga tcacgccgcg ccatcggttc
     3541 cccgccggag aaatgcgcct gcagaatgcc aagctccgcc gcctggtcga tcacggaaag
     3601 ccaggtttcg gtcggcagtt cgtcccccgc gcgctcaagc cgcagcgggt tggagcagta
     3661 ggggcattgc agcggacagc gatgcgtgag ttccagcagc attcccatcg gcgggtcggg
     3721 acgctgcatg cctgccacat cctgtgtggc cggcagatca tggggcatta cgtgactccg
     3781 ctcacacggc ccgcgtgggg atggcacgac gccctgcggc gccgtgccgc ctcatatcac
     3841 ttcgcgcggg cgcaggcgta gcagttgatc tcgccaccga gcgcgacttc gacgatcttc
     3901 ggggtggtcc aggccatgat gcggtcctcc gggttatggg ggcgatgtgg tgcccaaccc
     3961 gttctcatac gcgggcggcc ccgccagtca agcggcgttg cacgatggtc tgatcgggaa
     4021 tgccgcgcaa aacgaatcaa cccacgctca tcaggctggc attccccccg gccgccgcgg
     4081 tgttggtgct gaccgcccgt tcgcccacca gcaccgcaag gtcgtagtcc gccgaaccct
     4141 ccagcaccga cccggccggc ggcgccagca ccggcaccac cgggccggtg ccggccgcga
     4201 gcacgccgag ccagtggcgc aggctctcgg cgtcgccgtc atacagcacc gcctcgcacg
     4261 ccacgccgcg cacgtcgtcc acccgttgca cgcgcccgcg cagggcgggc ggcaggcggg
     4321 gcaggaagtc gttggggttg tgcggcggca gcacgatggc gctgttgccg cctgccagcg
     4381 ccgcgccgac ctgcgccacc atcccctccg gcgtcgcggc caggcacagc acccgcccgc
     4441 gcggctccag cgcgtagaca ttgcgctcgc cgaccgggcc gggcaggtcc accgtcgcgc
     4501 cgaccgcgct ctgcgccagc gcgcgctcgc aggcccgtat cgccgcctcg gtggtgaatg
     4561 gcctcgcgaa ctcgatccag gtacgcgccg ccgccgcatg cggcggctcc gccggcttcg
     4621 ccagcggcag cgccggcggc gcggcggcga gcaggcggcg caggatcagc gggccgcccg
     4681 ccttcggccc ggtgccggac aggccgtggc cgccgaaggg ctgcacgccg acgacggcgc
     4741 cgatcatgtt gcggttgacg tagatgttgc cgacgcgcgc gcgctcgacg atgcgatcga
     4801 tcgtcgcgtc gacgcggctg tgaa
//
LOCUS       DASSZH010000202         2725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_243543, whole genome shotgun sequence.
ACCESSION   DASSZH010000202 DASSZH010000000
VERSION     DASSZH010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243543"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFN42_04340"
     CDS             <1..205
                     /locus_tag="VFN42_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HET8995874.1"
                     /translation="TCGACIGGHMGALGPDEVCITASTRNFKGRMGDPSARIYMGSPA
                     TVAASALAGAIAHPGEFFRGDAA"
     gene            202..720
                     /locus_tag="VFN42_04345"
     CDS             202..720
                     /locus_tag="VFN42_04345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HET8995875.1"
                     /translation="MSGTNALAGRVWKFGDNVNTDLMLPGPYLFRTPEEQAQAVFINN
                     RPEFLREMHKGDVIIGGFNYGMGSSRPAARSLRNCGVGFLLAETINGLFFRNAVNFGF
                     RAFECPGVSQEFEEGDTAELSIEDWSATNRRTGRTLKVTPVPKMLLDLMVSGGVLPML
                     EAQGLIAPMRSA"
     gene            881..1867
                     /locus_tag="VFN42_04350"
     CDS             881..1867
                     /locus_tag="VFN42_04350"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET8995876.1"
                     /translation="MLPVSVVTGFLGSGKTTLIGRILRDPGFARTAVIVNEFGEIGLD
                     HTLIASSDETLLALTTGCLCCAVRSDLVATLLDLEQRREAGEIGYDRVLIETSGLADP
                     APILQALMTDPELGRTHTLDTVLTLADALLGEATLDRHPEARRQAAFADVVAISKTDL
                     APPDALRARLTALNPGARLMHSDALAPADLFTAADPALRAERLSTAARGMPSPFLRAE
                     HTDGLESFTLQRDQPMPALALTLLLQALVEHCGSRLLRVKGLVEIAEMPGQPALVQGV
                     QHVFSPPAFLPRWPSADRTTRMVFIARGVPRHFPARLLAAIEEEVRAAQAAG"
     gene            1932..>2725
                     /locus_tag="VFN42_04355"
     CDS             1932..>2725
                     /locus_tag="VFN42_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HET8995877.1"
                     /translation="MPAARLAVDIGGTFTDLALEHDGRRTTIKVLTTQAAPEQGVLDG
                     VRSILPAAGVNAADIAIVIHGTTLATNAVIERKGARTALITTEGFRDVIAMGNESRYD
                     QYDLNITLPEPLVPRHLRLTVPERLDNEGHVLRPLDEAAVHALVPVLQRKQVQSIAVG
                     LLHAFVNPEHERRVRGVLTQALPGVPVSLSSDVSPEMREWERFSTTVANAYVQPLMER
                     YLRRLEAGLRALHCTAPVFLMMSGGGLTTIETACRFPIRLVESGPAG"
BASE COUNT          415 a          962 c          925 g          423 t
ORIGIN      
        1 cacctgcggt gcctgcatcg gcggccacat gggcgcgctc gggccggacg aggtgtgcat
       61 caccgccagc acccgcaact tcaagggccg catgggcgat ccctcggcgc gcatctacat
      121 gggctcgccg gccaccgtgg cggcctcggc tctggccggc gccattgccc atccgggtga
      181 attcttccgg ggagacgcgg catgagcggc accaatgcgc tggccggacg ggtgtggaag
      241 ttcggcgaca acgtcaacac cgacctgatg ctgcccgggc cgtatctgtt tcgcactccg
      301 gaggagcagg cgcaggcggt gttcatcaac aaccgcccgg aattcctgcg cgagatgcac
      361 aagggcgatg tgatcatcgg cggcttcaac tacgggatgg gctcatcgcg ccccgccgca
      421 cggtcgctgc gcaattgcgg tgtcggcttc ctgctggccg agacgatcaa cggcctgttc
      481 ttccgcaacg ccgtcaattt cggcttccgc gccttcgaat gcccgggcgt cagccaggaa
      541 ttcgaagagg gcgatacggc cgaactgtcg atcgaggact ggagcgccac caaccgccgc
      601 accggccgca cgctgaaggt gacaccggtg ccgaagatgc tgctggacct gatggtgagc
      661 ggcggcgtgc tgccgatgct ggaagcgcaa ggcctgatcg caccgatgcg ctccgcgtaa
      721 atcgaagcaa gactcctccc cctccccttg ctggaggggg ttggggggag gggggtggtt
      781 ggccacgagc gcgggagctc atgcaggccc cctccccctg gccccctccc gcaaggggag
      841 ggggaatcgt tctccgcact cccatcggcg tcatcccctc atgcttccag tctccgtcgt
      901 caccgggttc ctgggcagcg gcaagaccac gctgatcggc cgcattctgc gcgaccccgg
      961 cttcgcgcgc accgccgtga tcgtcaacga attcggcgag atcggcctgg atcacacgct
     1021 gatcgccagc agcgacgaaa ccctgctggc gctgaccacc ggctgcctgt gctgcgcggt
     1081 gcgcagcgac ctggtggcca cgctgctgga cctggagcag cggcgcgaag cgggcgagat
     1141 cggctatgat cgcgtgctaa tcgagacctc cggcctggcc gatcccgcgc ccatcctgca
     1201 ggcgctgatg accgacccgg agctcggccg cacccacacg ctggacaccg tgctgaccct
     1261 cgccgacgcg ctgcttggcg aggcgacgct ggaccgccat ccggaagcgc ggcggcaggc
     1321 ggccttcgcc gacgtggtgg cgatcagcaa gaccgacctc gctccgcccg acgcgctgcg
     1381 ggctcggctg acggcgctga atcccggcgc ccggctcatg cacagcgatg cgttggcgcc
     1441 ggcggacctg ttcaccgccg ccgacccggc gctgcgggcc gaacggctga gcaccgctgc
     1501 gcggggcatg ccgtcgccat tcctgcgcgc cgaacacacc gacgggctgg agagcttcac
     1561 gctgcaacgg gaccagccaa tgcctgcgct ggcgctgacc ctgctgctgc aggccctggt
     1621 ggaacactgc gggtcgcggc tgctgcgcgt gaagggcctg gtcgagattg ccgagatgcc
     1681 cggacagccg gcgctggttc agggcgtgca gcacgtgttc tcgccgccgg cgttcctgcc
     1741 gcgctggcct tccgcggatc ggaccacgcg gatggtgttc atcgcccgcg gcgtgccgcg
     1801 ccacttcccg gcccgcctgc tggcggcaat cgaggaggag gtacgcgcgg cgcaggcagc
     1861 cggctgacac ctgcttgcca cgccctggcc ggcgcggttg actgccggcc ctgcattgtt
     1921 tcgaggattg gatgccagcc gctcgcctgg ccgtcgatat cggcggcacc ttcaccgacc
     1981 tcgcgctgga gcatgatggc cggcgcacca ccatcaaggt gctgaccacg caagccgcgc
     2041 ccgagcaggg cgtgttggat ggcgtgcgca gcatcctgcc tgccgctggc gtgaacgcgg
     2101 cggatatcgc catcgtcatc cacggcacca cgctggccac caacgcggtg atcgaacgca
     2161 agggtgcgcg cacggcgctg atcaccaccg aaggctttcg cgacgtcatc gcgatgggca
     2221 acgaaagccg ctatgaccag tacgacctga acatcacgct gcctgaaccg ctggtgccgc
     2281 ggcatttgcg cctgacggtc ccggaacggc tggacaatga aggccacgtg ctgcgcccgc
     2341 tggacgaagc cgcggtgcat gccctggtcc cggtgctgca gcgcaagcag gtgcagagca
     2401 tcgccgtcgg cctgctgcac gccttcgtca atcccgagca tgaacgccgc gtgcgcggcg
     2461 tgctgaccca ggcgctgccg ggcgtaccgg tatcgctgtc cagcgacgtg tcaccggaga
     2521 tgcgcgagtg ggagcgcttc tccaccaccg tcgccaatgc ctacgtgcag ccgctgatgg
     2581 agcgctacct gcgccgcctg gaagccgggc tgcgggcgct gcactgcacc gcgcccgtgt
     2641 tcctgatgat gtccggcggc ggcctgacca cgatcgagac tgcctgccgt ttcccgatcc
     2701 gcctggtgga aagcggcccg gccgg
//
LOCUS       DASSZH010000203         3153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1351081, whole genome shotgun sequence.
ACCESSION   DASSZH010000203 DASSZH010000000
VERSION     DASSZH010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351081"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..166
                     /locus_tag="VFN42_04360"
     CDS             <1..166
                     /locus_tag="VFN42_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosomal-protein-alanine acetyltransferase"
                     /protein_id="HET8995878.1"
                     /translation="AEAHHRGAATLTLEVAVDNAAARRLYESTGFTQVGRRKRYYADG
                     SDALVLQVAL"
     gene            complement(188..424)
                     /locus_tag="VFN42_04365"
     CDS             complement(188..424)
                     /locus_tag="VFN42_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HET8995879.1"
                     /translation="MSAADHEIDITGEVCPMTFVRTRLALDRMAPGQVLLVRLMGDEP
                     LRNVPRTATEQGHTVLSLEAGPDGVSLLRLRRGG"
     gene            521..937
                     /locus_tag="VFN42_04370"
     CDS             521..937
                     /locus_tag="VFN42_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HET8995880.1"
                     /translation="MPESPDSRDLLDLTVQIVAAHVSHNSVPPAAVPGMIEQVYRTLQ
                     DMGKPPPEPSRPSPAVPVNRSVFEDHIVCLEDGKRLKMLKRHLKAAYNLTPDEYRRRW
                     NLPASYPMVAPEYARRRSDLARQIGLGNHQPRRHRR"
     gene            1109..1612
                     /locus_tag="VFN42_04375"
     CDS             1109..1612
                     /locus_tag="VFN42_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HET8995881.1"
                     /translation="MVSRIERLCIERGLKMTGQRRVIARVLSEASDHPDVEELYRRAS
                     AIDAHISIATVYRTVRLLEENGILERRDFGGGRARYEPTEHGHHFHLIDVETGKVIEF
                     HDPEYERLMQTIAARLGFDLVSLRLELFGRRRQAAGSPRPAARRGPARVNGGQTNAEG
                     AEGGVPR"
     gene            1609..2448
                     /locus_tag="VFN42_04380"
     CDS             1609..2448
                     /locus_tag="VFN42_04380"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HET8995882.1"
                     /translation="MTAERPVGRFLVPTALPFPELRSGNMGVRIATTAAEIDAVQALR
                     YRVFYHEMGATADAETARLQRDRDQFDAIADHLLVVDHAIGGGAEGVVGTYRLIQREA
                     AERIGRFYSAGEYDISPINAFPGRVLELGRSCVDAAYRNRAAMQLLWRGIAAYVFHYQ
                     IDLMFGCASLPGTDPDALAVELTYLYQNHLAPPAVRPRALPHRYVEMCRVRADGIDPR
                     RVLAQLPPLIKGYLRLGGFVGDGAVIDPQFNTTDVAVVVKTDLVTDKYYRHYERQLRD
                     ALD"
     gene            2472..>3153
                     /locus_tag="VFN42_04385"
     CDS             2472..>3153
                     /locus_tag="VFN42_04385"
                     /inference="COORDINATES: protein motif:HMM:NF039484.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HET8995883.1"
                     /translation="MTRPATPAPQTGRIARSAARLRQLRGPQADLAAAVSGAVAALAF
                     PPMFALPVLFVCFPVLLILIDAAPRPAVAARRGWWFGFGMHLVGLYWITEAILVEAAK
                     FWWLIPFAVPALAAVLAAFIAAACWVARYAHRGWPRVLALAGAWTLADLARQFVATGF
                     PWNLLGSVWEFPGHLGDVFIQPAALVSVHGLTLATMFLVLVPMLGWCWRAAGAALFAA
                     WLAFGLYRM"
BASE COUNT          473 a         1093 c         1082 g          505 t
ORIGIN      
        1 ggccgaggcg caccaccgcg gcgcggccac gctgacgctg gaagtcgcgg tggacaatgc
       61 cgcggcgcgc aggctttacg aaagcaccgg cttcacccag gtcggccgcc ggaagcgcta
      121 ctacgccgac ggcagcgacg cgctggtgtt gcaagtggcg ctctgacgcg ggtgctggcg
      181 ccgcggatta tccgccacgc cgcagccgaa gcaggctgac accgtccggg ccggcctcca
      241 gcgacagcac cgtgtgtccc tgctccgttg ccgtgcgcgg cacgttgcgc agcggctcgt
      301 cgcccatcag ccgcaccagc agcacctggc ccggcgccat gcggtccagc gccagccggg
      361 tgcgtacgaa ggtcatcgga cagacctctc cggtgatgtc gatttcgtgg tccgctgcgc
      421 tcatgttgat ctcggaattg atattctgcc agctaggtac cattgcgaaa cggaggaaca
      481 gaacttagca tgccggaaaa gcctacccga agggattgca atgcccgaat caccggacag
      541 ccgtgacttg cttgatctga ccgtgcagat tgttgccgcc catgtgtcac acaattccgt
      601 tcccccagcc gccgtgcccg gcatgatcga gcaggtgtac cggacgctgc aggacatggg
      661 caagccgccg ccggaaccaa gccgccccag cccggccgtg ccggtcaacc gctcggtgtt
      721 cgaggatcac atcgtctgcc tggaagacgg caagcggttg aagatgctga agcggcatct
      781 gaaggctgcg tacaacctca cgccggacga gtaccgccgg cggtggaacc tgcccgccag
      841 ctacccgatg gtggcgccgg aatatgcccg ccgccgctcc gatctcgcgc gccagatcgg
      901 tctgggcaac caccagccgc ggcgtcatcg ccgctagcca tccgactcca cagcaggatt
      961 ggacagggcg cccccggatc ggctacgccc tgacggggcc ggtgcgggtc cggcagcctg
     1021 gcctgccgcc accgggcgca gacccggcac agcgcgtgcg gggccggtcg gaatgaaccg
     1081 gcggcttgat ggggtcgcaa ggaatcgcat ggtatcgcgg atcgagcgcc tgtgcatcga
     1141 acggggcctg aagatgaccg gccagcgccg ggtcatcgcg cgcgtcctgt cggaagccag
     1201 cgaccatccc gacgtggagg aactgtatcg gcgcgccagc gccatcgacg cccatatctc
     1261 gatcgccacc gtctatcgca ccgtccgcct gctggaggag aacggcattc tcgagcggcg
     1321 cgatttcggc ggcggacggg cgcgctacga accaaccgag cacggccacc acttccacct
     1381 gatcgatgtg gaaaccggca aggtcattga gttccatgat ccggagtacg agcggctgat
     1441 gcagacaatc gcagcgcgac tggggtttga cctcgtgtcg ctgagactcg agttgttcgg
     1501 ccgccgccgc caggctgcgg gttcgccacg cccggccgcg cgacgcggcc cggcacgggt
     1561 gaacggcggc cagaccaacg ccgagggcgc ggaaggcggg gtgccgcgat gaccgcggag
     1621 cgtccggtgg ggcgcttcct ggttcccacc gcgctgccct tcccggagct gcgcagcggt
     1681 aacatgggcg tgcggatcgc caccaccgcc gcggagatcg atgcggtaca ggcgctgcgc
     1741 taccgggtgt tctaccatga gatgggcgcc actgccgacg cggagaccgc ccgtctccag
     1801 cgcgaccgcg accagttcga tgccatcgcc gaccacctgc tggtggtgga tcacgccatc
     1861 ggcggcggcg cggaaggcgt ggtcggcacc taccggctga tccagcggga ggcggcggag
     1921 cggatcggcc gattctattc ggccggcgag tatgacataa gcccgatcaa cgcgtttccc
     1981 ggccgggtgc tggaactcgg ccgctcctgc gtcgatgcgg cctatcgcaa ccgcgccgcc
     2041 atgcaattgc tgtggcgcgg tatcgcggcg tatgtgttcc attaccagat cgatctgatg
     2101 ttcggctgcg ccagcctgcc gggaaccgat cccgacgcgc tggcggtgga gctgacctac
     2161 ctgtaccaga accacctcgc cccaccggcg gtacggccgc gcgcactgcc gcatcgctac
     2221 gtggaaatgt gccgggttcg cgccgacggc atcgatccgc gccgcgtgct ggcgcagcta
     2281 ccgccgctga tcaaaggcta tctgcggctg ggcggcttcg tcggcgacgg cgcggtgatc
     2341 gatccgcagt tcaacaccac cgacgtcgcg gtggtggtga agaccgatct ggtcaccgac
     2401 aaatactacc ggcattacga gcggcaactc cgggatgcgc tcgactgagc atggatgacg
     2461 cagggcccag cttgacccgg cccgccacac ccgccccgca gaccggccgc atcgcgcgga
     2521 gcgccgcccg cctgaggcaa ctgcgcgggc cgcaggcgga cctggccgcc gcggtctccg
     2581 gcgcggtggc ggcgctggcg ttcccgccaa tgttcgcgtt gccggtgctg ttcgtctgct
     2641 tcccggtgct gctgatcttg atcgacgccg caccgcgccc ggcggtggcc gcgcgccgcg
     2701 gctggtggtt cggcttcggc atgcacctgg tcgggctgta ctggatcacc gaggcgatcc
     2761 tggtggaggc cgccaagttc tggtggctga ttccctttgc cgtgccggcg ctggcggccg
     2821 tgctggccgc cttcatcgcg gccgcctgct gggtggcacg ctacgcgcat cgcggctggc
     2881 cgcgggtgct ggcgctggcc ggcgcctgga ccctggccga cctggcgcgg cagttcgtcg
     2941 ccaccggctt cccgtggaac ctgttgggca gcgtctggga attccccggc catctgggcg
     3001 acgtcttcat ccagccggcg gcgctggtca gcgtgcacgg gctgaccctg gccacgatgt
     3061 tcctggtcct ggtgccgatg ctcggctggt gctggcgtgc cgccggtgcc gcgctgttcg
     3121 ccgcctggct ggccttcggc ctgtaccgca tgc
//
LOCUS       DASSZH010000204         4440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_447840, whole genome shotgun sequence.
ACCESSION   DASSZH010000204 DASSZH010000000
VERSION     DASSZH010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447840"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..1086
                     /locus_tag="VFN42_04390"
     CDS             43..1086
                     /locus_tag="VFN42_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HET8995884.1"
                     /translation="MVAAFQDEERCRRLLEAMIWPRGRICPACGCKESTALAGREVGR
                     RARPGLYQCTNRACRYQFTVTTRTPLHATKLPLRVWLTGLWLILQSDKGVSSVRLAEA
                     IGVSQPTAWRMGHALRLLVTREHPLDGTVEMDEFHFGGTPRKDADRPRLGRGRKGQPR
                     TTKTPALAIVQRPAEIEEGAAAGEARARVVADLSEREARRVLGEHVDVNTHLMSDEWK
                     AFVSIGEAFVAHDTVRHSDREYARGFVHANSVEGFNDRVRRTVAGVFHHISPHHADLY
                     FNEVGFRWSQRVVTGQAVRRTRKGREVVKPLWSRIAPALQLPAVFKSAVGRQLRRTTH
                     GGIQIRCAVAVFG"
     gene            1422..1775
                     /locus_tag="VFN42_04395"
     CDS             1422..1775
                     /locus_tag="VFN42_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043743199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET8995885.1"
                     /translation="MRNLPQAWADDPARRARAGVPEDVAFQTKPQIALRLAKLRWRIE
                     RDYQDLKQELGLGHYEGRGWRGFHHHATLCIAAYGFLLAERAALPPSGEPRPSFLPQP
                     ALPEGYRPRGAPGPA"
     gene            complement(1933..2703)
                     /locus_tag="VFN42_04400"
     CDS             complement(1933..2703)
                     /locus_tag="VFN42_04400"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET8995886.1"
                     /translation="MARRNAAGTPRAPARMSDVLDRVRSVTPAAVVSKARHAYEVLRG
                     EILDGVHPPGSWLRLTPIAERLLLSEMPVREALRLLETDGLVTIHLHRGAQVAGLSFA
                     RALEITEARMCLEAAAAVAATPHHDAASLGAAEAILGGMRALGRNRAEFALRNRQFAA
                     AIYAPCPNAFLREHVQYLWDQVWQYSSVAVFEVMRHRVADSLEENRDICTAIRARDVA
                     AVRDIYAVRLQRSVDAWQRAIANKQARTAHAPSDRPQP"
     gene            2843..3523
                     /locus_tag="VFN42_04405"
     CDS             2843..3523
                     /locus_tag="VFN42_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HET8995887.1"
                     /translation="MGKPWDGIIPEEEQRAYHAAGFGRSGGLGKRPALLIIDVQYRTV
                     GTVPRPFWEAIKEFPTSCGEVGWAAVARIRLLLDLFRSRGWPVLYPHVAPKLAFDQGR
                     LAAKVPALMGVAAKGYEFVPDIAPGPDDVLLPKKHPSAFFGTPLASYLIDCGADTLVV
                     TGCTTSGCVRGSVVDGFAYNFRMLVPHDAVYDRSATSHAVNLFDMASKYADVMTTEEA
                     LALLRAVP"
     gene            3544..>4440
                     /locus_tag="VFN42_04410"
     CDS             3544..>4440
                     /locus_tag="VFN42_04410"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8995888.1"
                     /translation="MQNDSLKITRRKVLAGMGAAGAAIASRRAAAEPAPLRFGLAMPL
                     TGGQATYGQDQVRAAQWALDAINGAGGVNGRKLEAITLDTQADAQIGIQAANRLVSVE
                     NVPVFVTAWSEVVKAVAPVANDAKVVELSVGANSAQIAKLGEYVYTTFPLADVDITAV
                     ARYEAATMGKKRAAVLYINNETGIVAAEIYRKVFTQAGGSVVAYEAYDPKATDWTGQI
                     LKVRLASPDVIHIQGLVADTPQVIAQLRQLGMTQTISSYSSIYNPRLIRQLGAAAEGV
                     IATSLAPGPTDNPAVKAYVERWK"
BASE COUNT          736 a         1493 c         1501 g          710 t
ORIGIN      
        1 gaagtgcggg agattttggc ggggatcaac acggttggcg atatggtcgc tgcgtttcag
       61 gacgaggagc gttgtcgacg cctgctggag gcgatgatct ggccgagagg ccgcatctgc
      121 ccggcgtgtg gatgcaagga atcgacggca cttgcgggac gtgaggtcgg ccggcgtgcc
      181 cggcccggcc tctatcagtg cacaaatcgc gcgtgccgct accagttcac cgtgacgacg
      241 cggacaccgc tgcacgcgac caagctcccc ctgcgggtct ggttgaccgg cctatggctg
      301 atcctgcagt ccgacaaggg cgtttcctcg gtgcgcttgg ctgaggcgat cggcgtcagc
      361 caaccgaccg cctggcgcat gggccatgcc ttgcggcttc tggtgacccg tgagcatccg
      421 ctcgacggca cggtggagat ggacgagttc catttcggcg gcacgccgcg caaggacgcc
      481 gatcggcctc gtctgggacg cggacgcaaa ggccagccgc gaaccaccaa gacaccggct
      541 ctcgcgatcg tccaaagacc ggcggagatt gaagaagggg cagcggccgg cgaagccaga
      601 gcccgcgtcg tcgccgatct gtcggagcgc gaggcgcgcc gggtgctggg cgagcatgtt
      661 gacgtgaaca cgcatctgat gagcgacgaa tggaaggcgt tcgtctccat cggcgaagcc
      721 tttgtcgccc acgacaccgt ccgtcattcg gatcgggaat atgcccgcgg cttcgtgcat
      781 gccaactcag tcgaggggtt caatgatcgt gtccgccgca ccgtcgccgg cgtcttccat
      841 cacatcagcc cccaccacgc cgacctgtac ttcaacgagg ttggctttcg ctggtcgcaa
      901 cgggtcgtga ccggacaagc ggtgcgccgg acgcgcaagg gcagagaggt cgtcaagccg
      961 ctgtggtccc gaatcgcacc cgccctgcaa ttgccggctg tgttcaagtc cgccgtcggg
     1021 cgccaattgc gccgaaccac acacggtgga atccagatcc gctgtgccgt tgctgtcttt
     1081 ggttgataaa ggcggcgtac cgcgaggccg tttcgctagt tccggccaga tactcggcaa
     1141 ccgtgtagcg gctgacgccc accgccgcgg caatctgccg ggcactctgc ccgcagacgt
     1201 gcttgagacg aaggacctct cgaacctgac gcatcggctg tctctcggct ggcatggcgc
     1261 ttcccctgct gtgggaaagc agcctgccgt cggttgatga ctgccctcag gtcacgccgt
     1321 tcaccccttc agggtggctg gcttgcctcg gaatgggtgg ctggcttgtg tcggaatcac
     1381 cggccggctt ccatcggaat gcccggctgc tttgcgtcgg aatgcgcaac ctgccgcagg
     1441 cgtgggccga cgatccggcg cggcgcgcac gggcgggcgt gccggaggat gtggcattcc
     1501 agaccaagcc gcagatcgca ctcaggctcg ccaagctgcg ctggcgcatc gagcgggact
     1561 accaggacct caagcaggaa ctcggtctcg gccattacga aggtcgcggc tggcgcggct
     1621 tccatcacca cgcaacgctg tgcatcgccg cctacggctt cctcctcgcc gagcgggcgg
     1681 cgcttccccc ctccggagaa ccaagaccgt cattcctccc gcaacctgcc ctacccgaag
     1741 gttaccgacc gcgcggcgct cccggtccgg cctgagcgcc acgccgtcac gtcgataacc
     1801 tcggtccaca tctccattgc ccgcgccctg gcgcgaacac tcccgcgctg cccatgttgt
     1861 cagcgtcagc atagcaagca atttatgaca cagtagtact agcccggcgt ataggcgcat
     1921 gtttggtccc agtcagggct gcgggcgatc cgacggggca tgcgccgtcc tggcctgctt
     1981 gttggcgatg gcgcgttgcc aggcatcgac cgaccgttgc agccgcacag cgtagatgtc
     2041 gcgcaccgcc gccacgtcgc gcgcgcggat cgccgtgcag atgtcgcggt tctcctcgag
     2101 cgaatccgcg acgcggtggc gcatgacctc gaacaccgcg accgacgagt attgccacac
     2161 ctggtcccag agatactgca cgtgttcgcg caggaacgca ttggggcagg gggcgtagat
     2221 cgcggcggcg aactgccggt tcctcagcgc gaactccgcg cggttccgcc cgagcgcgcg
     2281 catcccgccc aggatcgcct cggcggcgcc gagcgaggcc gcatcgtgat gcggcgtcgc
     2341 cgccaccgcc gcggcggctt ccaggcacat gcgcgcctcg gtgatctcca gcgcccgcgc
     2401 aaaggacagg cccgccacct gcgcgccgcg atgcaggtga atggtgacca gaccatccgt
     2461 ctccagcagg cgcagcgcct cccgcacagg catctcgctg agcaacaggc gctcggcgat
     2521 gggggtcagg cgcagccacg accccggcgg atgcacgccg tccaggatct ccccacgcaa
     2581 cacctcatag gcatgacgcg ccttggagac gacggcggcg ggcgtgacgc tgcgcacgcg
     2641 gtccagcacg tcgctcatcc gcgccggggc gcgtggtgtc cccgcagcat tccgtcgtgc
     2701 catgcgcaac gtcttccgta tccgatccca acgaagggat tgaccgatcc cgtccaaccg
     2761 gatcatatca aatatcagat cctggcgaca accgccgcac cggcgtcggc cggcagagga
     2821 tggagaagga aggcgtccgg tcatgggcaa gccatgggac ggcatcatcc cggaagagga
     2881 acagcgcgcc tatcacgccg ccggtttcgg ccggtccggc gggctcggca agcggccggc
     2941 gctgctgatc atcgatgtcc agtatcgtac ggtcggcacg gtgccgcgcc cgttctggga
     3001 agcgatcaag gagttcccaa cgtcctgcgg cgaggtcgga tgggcggcgg tggcgcgtat
     3061 ccggctgctg ctggatctgt tccgcagccg cggctggccg gtgctgtatc cccatgtcgc
     3121 gccgaagctc gccttcgacc agggacggct ggccgccaag gttcccgcgc tgatgggcgt
     3181 cgccgcgaaa ggctatgagt tcgtgccgga tatcgcgccg gggcctgacg acgtcctgct
     3241 gccgaagaag catccgagcg cgttcttcgg cacgccgctg gccagctacc tgatcgactg
     3301 cggcgccgac acgctggtgg tgaccggctg caccaccagt ggctgcgtgc gcggcagcgt
     3361 ggtggacggg ttcgcctaca acttccgcat gctggtgccg cacgacgcgg tctatgaccg
     3421 cagcgcgacc tcgcacgccg tcaacctgtt cgacatggcg tccaaatatg ccgacgtgat
     3481 gacgacggag gaggcgctgg cgctgctgcg cgccgtgccg tgaccgtcgc aggtgaggaa
     3541 acgatgcaga acgacagcct gaagatcacg cgccgcaagg ttctcgccgg gatgggtgcg
     3601 gccggtgcgg cgatcgcgtc gcgccgcgcc gccgcggaac cggcgccgct gcggttcggt
     3661 ctggcgatgc cgctgaccgg cgggcaggcg acctatggcc aggatcaggt gcgcgcggcg
     3721 caatgggcgc ttgacgcgat caacggcgcc ggcggcgtca acggccgcaa gctggaggcg
     3781 atcacgctgg atacccaggc cgacgcgcag atcggcatcc aggcggcgaa ccggctggtg
     3841 tcggtcgaga acgtgccggt gttcgtcacc gcctggtcgg aagtggtgaa ggcggtcgcg
     3901 ccggtcgcga acgacgcgaa ggtcgtcgag ctgtcggtgg gcgccaactc cgcgcagatc
     3961 gcgaagctgg gcgagtatgt ctacaccacg ttcccgctcg ccgatgtcga tatcaccgcc
     4021 gtcgcgcgct acgaagccgc gacgatgggc aagaagcgcg cggccgtgct ctacatcaac
     4081 aacgaaaccg gcatcgtcgc ggcggagatc taccgcaagg tgttcacgca ggccggcggg
     4141 tcggtggtcg cctacgaggc gtacgacccg aaggcgaccg actggaccgg gcagatcctg
     4201 aaggtgcggc tggcgtcgcc ggacgtgatc catatccagg ggctggtcgc cgacacgccg
     4261 caggtgatcg cgcagctgcg ccagctgggc atgacacaaa cgatctcctc ctactcctcg
     4321 atctacaatc cgcgcctgat ccgccagctt ggcgcggcag ccgagggggt gatcgccacc
     4381 tcgctggcgc ccgggccgac cgacaatccg gcggtgaaag cctatgtcga gcgctggaag
//
LOCUS       DASSZH010000205         5376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1197891, whole genome shotgun sequence.
ACCESSION   DASSZH010000205 DASSZH010000000
VERSION     DASSZH010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197891"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..1866
                     /locus_tag="VFN42_04415"
     CDS             211..1866
                     /locus_tag="VFN42_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995889.1"
                     /translation="MNAAAPAPACHVVFRLSRRADGSETACYEVPDEAVPLDEATWPH
                     WLARRELLGARQLSVPVLVLRETLDLSGLVAECTLERRLGAVDGPFCGNPARVDFAGP
                     ARMVRLYRVLRDGQLAQMGLSPQVLPPPAARGAEPFAGELQALERRFGPGLQVLDARE
                     PTLFEDVAGLASIIHIRLPDGRELACGIGWHGTTPIILVADFPRFDVALALPPVEETA
                     VQDALRRLGLVRHETRMDAAGLAVLARRRGGQELLLLRIAGARCSAEPYQPGTAQLGS
                     PDQRGWLRYAEVHEGLKPLDLYAEGASGDLIALTGDASGHVWRHLVDADGVEAWRRPA
                     DDAAVAALHREALAPGTLLRLEGPSADPDLARAEQPAEPADAAPGGGDPDGLARELAR
                     LRDDLAQIRLMPLQPPWQPPHDAAPFGAEVEARFPDCAYHAEEAASCLAFRRPTAAVF
                     HCMKVVERGLAALGRLLGMDGFPGRVRGWGTIMGAVRPACPPELSPVLGHLQRIRQRW
                     HAPGLDPAEKYTEAEAEQVFQAVDAFMRALAERYDEAGSQGGN"
     gene            2104..2466
                     /locus_tag="VFN42_04420"
     CDS             2104..2466
                     /locus_tag="VFN42_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8995890.1"
                     /translation="MQNQRRSILVVEDDPEISELLREALAERYAAECVGNVAAALARL
                     TERAPDVILMDCQLPGGDAMDVVDKARNIACPMVLTSGLPEVLEALSVFGHPCLPKPF
                     RIGQLMAAIEAAVSRNGK"
     gene            complement(2508..2993)
                     /locus_tag="VFN42_04425"
     CDS             complement(2508..2993)
                     /locus_tag="VFN42_04425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8995891.1"
                     /translation="MPAARRAERSDLESLLALLRASEVSAVADSPEHRDRIWSETLAR
                     DGLVVFVANASARIVASCMLITAPNLLRGGRAHGFLENVVTHPEHRGQGYGRAVVQAA
                     LNEAWARDCHHVLLQSGRQDPRVHRFYQGCGFEPGVRTAYVARRPPEHALTASAAARG
                     S"
     gene            complement(3123..4094)
                     /locus_tag="VFN42_04430"
     CDS             complement(3123..4094)
                     /locus_tag="VFN42_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HET8995892.1"
                     /translation="MAPKILVADNPLNENDIARELAPPGFDTIIARPGSPEFNAALPD
                     AEYIVGFGDNTMNDAFYQNAPKMKLVQLLSAGYDRCDIEAARRAGVPICNNGGANAIA
                     VAEHALLLMLAVSRRLVWQHDNVAAGRWRGNNWTDVKLYELYGRTLGIIGLGTIGKKV
                     ARLGKAFGMDVQYYDIARLTEDQTDALSVRFRLMDEILRSSDIVSLHVPLTTLTKHMI
                     GAAQLRLMKPTAYLINTSRGPVVDEPALIAALSDNTIAGAGLDVFDQEPPPSDNKLFG
                     LPNVILSAHFAGPTWDNQYARFRNAFDNVQRVARGEKPLWVIPELRA"
     gene            complement(4171..>5376)
                     /locus_tag="VFN42_04435"
     CDS             complement(4171..>5376)
                     /locus_tag="VFN42_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014106328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF/VisC/COQ6 family ubiquinone
                     biosynthesis hydroxylase"
                     /protein_id="HET8995893.1"
                     /translation="CIMGAGPVGGTLAARLAQAGVRTVVVDRAALPPMEHPEFDGRAY
                     AIAAGSRGLLAAAGLWEHLPEPPCPILDIRVSDGRLGRPASRLHLHFDHREAGEAAGP
                     FGWMVEARSLRRALNAHLHALPALTVFAPAEAQVERSADGVTVRIAGGPEVQCRLVVA
                     AEGRQSPLRESAGIPLTRFPYHQTGIVVAVSHEKPHHNVALEHFLPSGPFAQLPMSAS
                     PDAEAGGAPHVSAIVWTERTPTAQQMLRLDEARFAREIGRRLGDHLGAVRTVGRRWSY
                     PLSAMIAHRYVDTRLALAGDAAHGIHPIAGQGLNLGFRDVIALAELVIEAVERGEDPG
                     SPELLTRYQRRRRPDNMLMLAMTDGLDRLFSNDFPPLRMARDLGIAAVHRMGPLKRVF
                     MRQAMGLGG"
BASE COUNT          799 a         1840 c         1898 g          839 t
ORIGIN      
        1 gcctctcgcc gggaaatcgg caaaatcgat ccgaaacccc acgtatccga tcgtgaaagg
       61 aatcaggaac gcgcgtcatg ctaagcggac gattctgcgg taaccaagac cgccgcaacc
      121 tttggttgac ggaaacgcca cgcatcaatg agctctgctg atcaaggtga ggtctggact
      181 gggccacagc ttgccgcgca acttgcggcc gtgaatgctg ccgctccggc gccggcctgc
      241 cacgtggtgt tccgcctgtc ccgccgcgcc gacggcagcg aaaccgcgtg ctacgaggtt
      301 cccgacgagg ccgtgccgct ggacgaggcg acttggccgc actggctcgc ccggcgcgag
      361 ctgctgggcg cccggcagct cagcgtgccc gtgctggtgc tgcgggaaac gctcgatctg
      421 tccggccttg tcgcggaatg cacgctggag cggcgcctgg gagcggttga cggcccgttc
      481 tgcggcaatc ccgcccgggt cgatttcgcc ggcccggcgc gcatggtccg gctgtatcgc
      541 gtgctgcgcg acgggcagct tgcccagatg gggctgtcgc cgcaggtcct gccgccgccc
      601 gcggcgcgcg gggcggagcc cttcgccggg gagcttcagg cgctggagcg ccgcttcggg
      661 ccagggctgc aggtgctcga tgcccgcgaa cccaccttgt tcgaggatgt ggcggggctt
      721 gccagcatca tccacatccg cctgccggac gggcgggagc tggcctgcgg cattggctgg
      781 cacggcacga cgccgatcat cctggtggca gacttcccgc gcttcgacgt cgcgctggcc
      841 ctgccgccgg tggaggaaac cgcggtgcag gacgcgctgc gccgcctggg tctggtgcgg
      901 catgaaaccc ggatggatgc ggccggcctg gccgtgctgg cgcgccggcg cggcggacag
      961 gagctgttgc tgctgcgcat cgccggcgcg cgctgctccg cggaacccta ccagcccgga
     1021 accgcccagc tcggcagccc ggatcagcgc ggctggttgc gctacgccga ggtgcatgag
     1081 gggctgaaac cgctggatct gtatgccgaa ggcgcctcgg gtgacctgat cgcgctgacc
     1141 ggcgatgcca gcggtcacgt gtggcggcac ctggtggacg ccgatggggt ggaggcctgg
     1201 cgccggccag ccgacgacgc cgcggtggca gcgctgcatc gcgaagccct ggcgccgggg
     1261 accctgctcc ggctggaggg tccgtctgcc gatcccgatt tggcgcgggc ggagcagccg
     1321 gccgagccgg cggacgcggc gccgggcggc ggcgatccgg acggtctggc acgggagctg
     1381 gcgcgcctgc gggacgatct ggcgcagatc cgcctgatgc cgttgcagcc gccatggcag
     1441 ccgccgcacg acgcggcccc gttcggcgcc gaggtggagg cgcggttccc cgactgtgcc
     1501 tatcacgctg aggaagccgc gagctgcctg gccttccgcc gcccgaccgc cgcggtgttt
     1561 cattgcatga aggtggtgga gcgcgggctg gcggccctgg ggcggctgct ggggatggat
     1621 ggctttcccg gccgggtgcg cggctggggc acgatcatgg gcgcggtgcg ccccgcctgc
     1681 ccgcccgaat tgtcgcccgt gctcggccac ctgcagcgca tccgccagcg ctggcacgcc
     1741 cctgggctgg acccggcgga gaaatacacc gaagccgagg cggagcaggt gttccaggcg
     1801 gtggatgcct tcatgcgggc cctggcggag cgatacgacg aggcgggttc ccagggcgga
     1861 aattagcgcc cgggtttccc tgtccgcttg cagtccagac ggatcgatgt gcgcgcgcgt
     1921 gcaggaacaa tcagcactcc acacattccg ctgccaaaaa tatacccggc aataatgccg
     1981 taactgcgtt gcgatgcgtg cggagcatcc ccagcttcgc atcgtcacta ggacgagcag
     2041 ttcggggcgc tagcgactga gagagacgca ctctccctgg aatcatctcg cagggaggat
     2101 tgcgtgcaga accagcgtcg ttccattctc gtcgtcgaag acgatccgga aatttccgag
     2161 ctgttgcggg aggcattggc cgaacgctat gcggccgagt gcgtcggcaa tgttgcggcg
     2221 gcgctcgcgc ggctgaccga acgcgccccg gatgtcattc tgatggattg ccagttgcct
     2281 ggcggcgatg cgatggacgt ggtggacaag gcgcggaaca tcgcctgccc catggtgctg
     2341 acatccggtt tgcccgaggt gctggaagcg ctgtcggtgt tcgggcatcc ctgtctgccg
     2401 aagcccttca ggatcggaca gttgatggcc gcaatcgaag ccgcggtctc acgcaacggg
     2461 aaatagcccg agccggcccg caaacatgtg cgccggcggc gtgcgactca cgaaccgcgc
     2521 gccgcggcag acgcagtcag tgcatgctcc ggcggtcggc gtgcgacata cgcggtccgg
     2581 acgcccggtt cgaagccaca accctgatag aagcgatgga cacgcggatc ctgccggccg
     2641 ctttgcagca gcacgtggtg gcagtcgcgc gcccaggcct cgttgagcgc ggcctgcacg
     2701 accgcgcggc cgtagccttg gccgcgatgc tccggatggg tgacgacgtt ttccaggaac
     2761 ccgtgtgccc gcccgccgcg cagcagattg ggcgcggtga tcagcatgca gctcgccacg
     2821 atcctggcgc tcgcgttcgc cacgaagacg acgaggccat cgcgcgcaag cgtctcggac
     2881 cagatgcgat cacgatgttc cggcgagtcc gcgaccgcgc tcacttccga cgcccgcagg
     2941 agagcgagca gggattccag gtcggagcgt tcggcgcgtc gggctgcggg catgattgaa
     3001 cctttctgag ccatgcccgg ccagggatgg catgccggcg gcccataggt cggatccgtt
     3061 gcgtcggagc gtctcctccc ccgtgcatga cacggaggag gcggaacatt cggccgttgg
     3121 tattacgcgc gcagttcggg gatgacccac agcggcttct cgccgcgcgc cacccgctgc
     3181 acgttgtcga acgcgttgcg gaaacgggcg tactggttgt cccaggtcgg gccggcgaaa
     3241 tgcgcggaca ggatcacgtt gggcaggccg aacaacttgt tgtccgaggg cggcggttcc
     3301 tggtcgaaca cgtccagccc ggcgccggca atggtgttgt cggacagcgc cgcgatcagc
     3361 gccggctcgt ccaccaccgg gccgcggctg gtgttgatca ggtaggcggt cggcttcatc
     3421 agccgcaact gtgccgcgcc gatcatgtgc ttcgtcagcg tcgtcagcgg cacgtgcagc
     3481 gagacgatgt cggagctgcg caggatctcg tccatcagcc ggaagcgcac gctgagcgcg
     3541 tcggtctggt cttcggtgag acgggcgata tcgtagtact gcacgtccat gccgaaggcc
     3601 ttgccgagcc gggcgacttt cttgccgatg gtgcccaggc cgatgatgcc cagcgtgcgg
     3661 ccgtacagct catacagctt cacgtcggtc cagttgttgc cgcgccagcg gccggcggcg
     3721 acattgtcgt gctgccacac cagccggcgg ctgaccgcga gcatcagcag cagcgcgtgc
     3781 tcggccacgg cgatcgcgtt ggcgccgcca ttgttgcaga tcggcacgcc ggcgcggcgc
     3841 gccgcctcta tgtcgcagcg gtcgtagccg gcggacagca gttgcaccag cttcatcttc
     3901 ggcgcgttct ggtagaacgc gtcgttcatc gtgttgtcgc cgaagccgac gatgtactcg
     3961 gcgtccggca gcgcggcatt gaactccggc gagccggggc gggcgatgat cgtgtcgaag
     4021 cctggcggcg ccagttcgcg cgcgatgtca ttttcgttga gcggattatc ggcgacgaga
     4081 atcttgggcg ccatcggcgt ttcctctccc caatgcggtt gccggccacg atacaggcga
     4141 ggctcttagc tggatagcgg gaggcgaggc tcacccgccc agccccatcg cctgccgcat
     4201 gaacacccgc ttgagcggtc ccatccggtg aaccgcggcg atgcccagat cgcgcgccat
     4261 ccgcagcggc ggaaaatcgt tgctgaacag ccggtccagc ccgtcggtca tcgccagcat
     4321 cagcatgttg tccggccgcc gccggcgctg atagcgcgtc agcaattccg gagaaccggg
     4381 atcttcgccg cgctccaccg cctcgatcac cagctccgcc agcgcgatca catcgcggaa
     4441 ccccaggttc agcccttgcc cggcgatcgg gtggatgccg tgcgccgcgt cgcccgccag
     4501 cgccagccgc gtatccacgt agcgatgcgc gatcatcgcc gacagcggat agctccagcg
     4561 ccgcccgacc gtgcgcaccg cgcccaggtg gtcgcccagc cggcgcccga tctcccgggc
     4621 gaagcgcgcc tcgtccagcc gcagcatctg ctgggcggtc ggcgtgcgct cggtccacac
     4681 gatggcggag acatgcggcg cgccgccggc ttcggcatcc gggctggccg acatcggcaa
     4741 ctgcgcgaag gggccggacg gcaggaaatg ctccagcgcc acgttgtggt gcggcttctc
     4801 gtggctgacc gccaccacga tgccggtctg atgatagggg aagcgggtca gcggaatgcc
     4861 cgcggactcg cgcagcggcg attgccggcc ctcggccgcc accaccagcc ggcattgcac
     4921 ctccggcccg ccggcgatgc gcaccgtgac cccatcggcg ctgcgttcca cctgcgcctc
     4981 ggccggcgcg aacaccgtca gcgccggcag cgcgtgcaga tgcgcgttca gcgcccggcg
     5041 caggctgcgc gcctccacca tccagccgaa tggaccggcg gcctcgccgg cctcccggtg
     5101 atcgaaatgc aggtgcagcc gcgaggcggg acgccccagc cttccgtccg agacgcggat
     5161 gtccaggatg gggcagggcg gttccggcag gtgctcccac agccccgccg cggcgagcaa
     5221 cccgcgggag ccggcggcga tggcataggc tcggccgtcg aattccggat gctccatcgg
     5281 cggcagggcg gcgcggtcca ccaccaccgt gcgcaccccg gcctgcgcca gccttgcggc
     5341 cagcgtgccg ccgaccggac cggcccccat gatgca
//
LOCUS       DASSZH010000206         4884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_584188, whole genome shotgun sequence.
ACCESSION   DASSZH010000206 DASSZH010000000
VERSION     DASSZH010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584188"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VFN42_04440"
     CDS             <1..877
                     /locus_tag="VFN42_04440"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HET8995894.1"
                     /translation="RLRSGLDLALDLTGAQPALTGTITAETLPLPLPLRTPGPLPIGL
                     LHGWQAKLRVKAAQLLGSGMTLLRDGSATLQLQHSVLSLEQLSGQLGGGALAGSLSLD
                     AGQQPPALTVQAKLTGAAIDGPVLRLPIDLSGGNADLSLSLRAVGYSTHALTASLVGS
                     VQASVRDGMLSGFDLAGLRDLLKLPGADPSEASLHNSLEGGQTAFHHLLLQGQVAHGI
                     LSLDTASLSGAAGGADATGTVTLPDATADLHLVLHPAIPQGPSIAMLVSGALGAPRRT
                     PELAGVARWRHARGH"
     gene            1095..3152
                     /locus_tag="VFN42_04445"
     CDS             1095..3152
                     /locus_tag="VFN42_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HET8995895.1"
                     /translation="MSLQSKLLWAGVSVLGAIAFAIVALTRGEPVNAAWLVIASVCIY
                     FIAYRFYALFITRKVFGVDVKRQTPAYRHNDGLDYVPTNRYVLFGHHFAAIAGAGPLV
                     GPVLAAQMGYLPGTLWILAGVVFAGAVQDMTVLFMSTRRDGRSLGDMIRSEMGPVPGA
                     IAGVGILLICIIILAVLALVVVNALKGSPWGTFAVFCTIPIALLMGVYTRFLRPGRIA
                     EMSVIGGLLLLAALIYGKTVADTPSLAAWFNLSGPNLALIIIGYGFVASVLPVWLLLA
                     PRDYLSTFLKVGTILLLAVAILIVRPDLQMAPITKFIDGTGPVWAGSLFPFLFITIAC
                     GAVSGWHSLISSGTTPKMVENEGQIRFIGYGGMLMESLVAIMAMIAASVIHPGVYFAM
                     NSAAGLIGTDPAHAAQVISGWGFTVTPDVLTAMAKDVGETTIMSRTGGAPTLAVGMAS
                     ILSGVLGGRALMGIWYHFAILFEALFILTTVDAGTRVCRFMIQDLVGNVVPAFRATES
                     WANNVVGSAAACVLWGYFLYQGVIDPLGGIWTLWPLFGTANQMLAAIALILCTVVLFK
                     MKRQHYAWVTIVPTTWLVACTVTAGLEKVFSANPAVGFLSHAHKFSASIAAGKVLAPA
                     KSLGEMGRVVFNDYVDATLAGMFAALVVAMVIYGIIHCRKAMGSPRSTAIEVGGLMAA
                     GDD"
     gene            3145..3360
                     /locus_tag="VFN42_04450"
     CDS             3145..3360
                     /locus_tag="VFN42_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HET8995896.1"
                     /translation="MTERLATRARLVCDLVTQTARLMVGVPDYQTYVTHRQTQHPGQP
                     VMTYQEFFRERQAARYAVGKGRFKGCC"
     gene            complement(3397..4350)
                     /locus_tag="VFN42_04455"
     CDS             complement(3397..4350)
                     /locus_tag="VFN42_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8995897.1"
                     /translation="MARSIAVGLGIMEFPFDGADGFWRWVDLCEAGGVDSLWQTDRLV
                     SRAPILESMTTLAAIAGRTRRLKFGVNVVSVAMREPVLLAKQCATIDMLSGGRLLPGF
                     GIGSPRGPEWAAMHLDPSTRGRKTDEALEIISRLWAGEALDYAGRHFQLTGAQIAPLP
                     AQPDLPMWIGGSSDAAVRRTARYGTGWQAGSEPPEEVGRTIAAIRAAVAECGRSIDED
                     HYGAAFAYRFGRWDDPGMGRVADAYRARTGRDAREAFAVGDAGSILERIAAYVAAGAS
                     KFILRPAAQGDADMLAQTRLLIEQVLPQVAARWPRPARSAA"
     gene            4545..>4884
                     /locus_tag="VFN42_04460"
     CDS             4545..>4884
                     /locus_tag="VFN42_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8995898.1"
                     /translation="MPAGPEPVTDATRVPYRGLITASAMLAMFMQTLDSTIANVALPY
                     MQGSMSASYDEITWVLTSYVIAAAIMTAPVGWMAVRFGRKRLFIICIAGFTIASMLCG
                     AAQSLEQLVGF"
BASE COUNT          684 a         1675 c         1709 g          816 t
ORIGIN      
        1 acgactgcgc agcggcctgg acctggcgct ggacctcacc ggcgcgcagc cggcgctgac
       61 cgggacgatc acggcggaga cgctgccgct gccactgccg ctgcggaccc cgggaccgct
      121 gccgatcggg ctgctgcacg gctggcaggc aaagctgcgg gtgaaggcgg cccagctcct
      181 gggatcgggg atgacgctgc tgcgcgacgg cagtgccacg ctacagttgc agcacagtgt
      241 cttgtcgctg gagcagttga gcggacagct gggtggcggc gcgttggctg ggagcctcag
      301 cctcgatgcg ggacagcagc cgccggccct gacggtgcag gcgaagctga cgggcgcggc
      361 gatcgacggc ccggtgctgc ggctgccgat cgacctgagc ggcggcaacg cggacctgtc
      421 gctgtcactg cgtgcggtgg ggtacagcac ccatgcgctg accgccagcc ttgtgggatc
      481 agtgcaggcc agcgtccgcg acggcatgct gtccgggttc gaccttgcgg gattgcgcga
      541 cctgctgaag ctgcccggtg ccgatccgtc ggaggcgagc ctgcacaact cgctggaggg
      601 cgggcagacc gcgttccatc acttgctgct gcaaggccag gtggcgcacg gcatcctgtc
      661 gctcgacacg gccagcctga gcggggccgc cggaggcgcg gacgcaaccg gcacggtgac
      721 gctgccggat gccaccgccg atctgcatct ggtgctgcat cccgcgatcc cgcagggccc
      781 cagcatcgcc atgttggtca gtggcgcatt gggagcgccg cggcgcacgc ccgaactggc
      841 gggggttgca cgctggcggc acgcccgcgg gcattgaacg ggtatcgccc ggccagtttc
      901 tcgcccactc agtgtggcgg accctgtcgg gagccgtcac cggcgcggtt tcgcggtgcc
      961 gtcacgcctg gcccattgac ccaggtcaat gcgccggcac cgcaacgtcg gcatagcgcg
     1021 ccgcagggcc aagccgagcg cgaacggcac ttctgcgccc acctcgccgg cgcccgtcgg
     1081 ttctggagcc aaccatgtcc ctgcagtcga aactgctatg ggccggggtg agtgtgctgg
     1141 gcgctatcgc cttcgccatc gtcgccctga cccggggcga gccggtcaat gcggcctggc
     1201 tcgtgattgc ctcggtgtgc atctacttca tcgcctaccg cttctacgcg ctgttcatca
     1261 ccaggaaggt gttcggcgtc gatgtgaagc ggcagacgcc ggcgtaccgg cacaacgacg
     1321 gcctggatta cgtaccgacc aaccggtacg tgctgttcgg ccaccacttc gccgccatcg
     1381 ccggcgccgg cccgctggtg ggcccggtgc tcgcggcgca gatgggctat ctgcccggca
     1441 cgctgtggat cctggccggc gtggtgttcg ctggcgcggt gcaggacatg accgtgctgt
     1501 tcatgtccac ccgccgtgac ggacgctcgc tcggcgacat gatccgttcc gagatggggc
     1561 cggtgcccgg cgccatcgcc ggcgtgggca tcctgctgat ctgcatcatc atcctggcgg
     1621 tgctggcgct ggtggtggtg aacgcgctga agggcagccc ctggggcact ttcgcggtgt
     1681 tctgcaccat cccgatcgcg ctgctgatgg gcgtgtacac ccgcttcctc cgccctggcc
     1741 gcatcgccga gatgtcggtg atcggcggcc tgctgctgct cgccgccctg atctacggca
     1801 agacggtggc cgacaccccg tcgctggccg cgtggttcaa cctcagcggg ccgaacctgg
     1861 cgctgatcat catcggctat ggtttcgtcg cctcggtgct gccggtgtgg ctgctgctgg
     1921 cgccgcgcga ctacctgtcc accttcctga aagtcggcac catcctgctg ctggcggtcg
     1981 ccatcctgat cgtgcggccg gatctgcaga tggcgccgat caccaagttc atcgacggca
     2041 ccgggccggt ctgggcgggc agcctgttcc ccttcctgtt catcaccatc gcctgcggcg
     2101 cggtgtccgg ctggcattcg ctgatctcgt cgggcaccac gccgaagatg gtggagaacg
     2161 agggacagat ccgcttcatc ggctatggcg ggatgctgat ggaatcactg gtggcgatca
     2221 tggcgatgat cgccgcatcg gtgattcatc ccggcgtgta tttcgcgatg aactccgcgg
     2281 cggggctgat cggcaccgat ccggcgcatg cggcccaggt gatctccggc tggggcttca
     2341 cggtgacgcc ggacgtgctg accgcgatgg cgaaggatgt gggcgagacc accatcatgt
     2401 cgcgcaccgg cggtgcgccg accttggcgg tgggcatggc gagcatcttg tccggcgtgc
     2461 tcggcgggcg cgcgctgatg ggcatctggt accatttcgc catcctgttc gaggcgctgt
     2521 tcatcctgac cacggtcgat gccggcacgc gcgtgtgccg cttcatgatc caggacctgg
     2581 tgggcaacgt ggtgccggcg ttccgggcga cggaatcctg ggccaacaac gtggtgggtt
     2641 ccgccgcagc ctgcgtgctg tggggctatt tcctctatca gggtgtgatc gatccgctgg
     2701 gcggcatctg gacgctgtgg ccgctgtttg gcaccgccaa ccagatgctg gcagccatcg
     2761 cgctgatcct gtgcaccgtc gtgctgttca agatgaagcg gcagcactac gcctgggtga
     2821 ctatcgtgcc gaccacctgg ctggtggcct gcaccgtgac cgccgggctg gagaaggtgt
     2881 tcagcgccaa cccggcggtg ggcttcctca gtcatgcgca caaattcagc gcctcgatcg
     2941 ccgccggcaa agtgctggcg ccggcgaagt cgctgggtga gatgggccgc gtcgtgttca
     3001 acgactatgt cgatgcgacc ctggccggta tgttcgccgc gttggtggtg gcgatggtga
     3061 tctacggcat catccactgc cgcaaggcga tgggctcgcc gcgcagcacc gccatcgagg
     3121 tgggcgggct gatggccgcg ggcgatgact gagcggctgg caacacgggc acggctggtc
     3181 tgcgatctgg tgacgcagac cgcccgcctg atggtcgggg tgccggacta tcagacctac
     3241 gtgacgcacc ggcagaccca gcaccccggc cagccggtca tgacctacca ggagtttttc
     3301 cgcgaacgcc aggctgcgcg ctacgcggta ggcaagggcc ggttcaaggg ctgctgctga
     3361 acattcgccg tggcgggcct ggcccgccac ggcgattcat gccgcgctgc gggcggggcg
     3421 cggccagcgc gcggcgacct gcggcaggac ctgctcgatc agcaaccgag tctgcgccag
     3481 catgtcggcg tcgccctgcg ccgccggccg caggatgaac ttcgaggcgc ctgccgcgac
     3541 ataggcggca atccgttcca ggatgctgcc cgcatcgccg acggcgaacg cctcgcgcgc
     3601 atcgcggccg gtgcgggcgc ggtacgcgtc cgccacgcgg cccatgcccg ggtcgtccca
     3661 gcggccgaag cgataggcga aggcggcgcc gtagtggtcc tcgtcgatgc tgcggccgca
     3721 ttctgccacg gcggcgcgaa tggcggcgat ggtgcggccg acttcctccg gcggttcgga
     3781 cccagcctgc cagccggtgc cgtagcgtgc cgtacggcgc accgcggcgt ccgatgagcc
     3841 gccgatccac atcggcaggt ccggctgcgc cggcagcggc gcgatctgcg ccccggtgag
     3901 ctggaaatgc ctgccggcat agtccagcgc ctcgcctgcc cacagccggc tgatgatctc
     3961 cagcgcctca tcggttttcc gcccgcgggt ggacgggtcc aggtgcatcg ccgcccattc
     4021 cggcccgcgc gggctgccga tgccgaaacc cggcaacagc cggccgccgg acagcatgtc
     4081 gatcgtggcg cattgcttgg ccagcagcac cggttcccgc atcgccaccg acaccacgtt
     4141 gacgccgaac ttgagccgcc ttgtccggcc ggcgatggcg gccagcgtgg tcatgctctc
     4201 caggatcggc gcacggctga ccaggcggtc ggtctgccac agggaatcca cgccgccggc
     4261 ctcgcacaga tcgacccagc gccagaaccc gtcggcgccg tcgaagggga actccatgat
     4321 gccgaggccg acggcgatgc tgcgcgccat gctaggatgc tccctgggcg ggtttccccc
     4381 ggatggtcgc ccggccatgc gcccgccgca agccggggcg gcgcggtgga cgcacggcgc
     4441 ggtggacgca gtagcgcgcg ccgggcaaac gcggcagact aggcgaatat caagccgtcc
     4501 aggaacaatg ccgcccagcc cgccccgccc gttgccaggc cgccgtgccc gccggccctg
     4561 agcccgtcac ggacgccacg cgcgttccct atcgcggcct gatcaccgcc tccgcgatgc
     4621 tggcgatgtt catgcagacg ctggattcca ccatcgccaa cgtggcgctg ccctacatgc
     4681 agggcagcat gtcggccagc tacgacgaga tcacctgggt gctgaccagc tacgtgatcg
     4741 ccgccgcgat catgaccgcg ccggtgggct ggatggcggt gcggttcggc cgcaagcggc
     4801 tgttcatcat ctgcatcgcc ggattcacca tcgcctccat gctgtgcggc gcggcgcagt
     4861 cgctggagca gctggtcggc ttcc
//
LOCUS       DASSZH010000207         4072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_328635, whole genome shotgun sequence.
ACCESSION   DASSZH010000207 DASSZH010000000
VERSION     DASSZH010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328635"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VFN42_04465"
     CDS             <1..385
                     /locus_tag="VFN42_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET8995899.1"
                     /translation="GRPELARDSDYATNGLRCQNRETLDTAIAAWTRTMAAKDAEAIL
                     EAAEVPCSRLFDIADCAADPHFRARKAVQEIDDPLIGRTLHPGPTIRLDGEPPEGVVR
                     WTGPAVGAHTDYVLHTLLGLPKASA"
     gene            413..979
                     /locus_tag="VFN42_04470"
     CDS             413..979
                     /locus_tag="VFN42_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006558529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HET8995900.1"
                     /translation="MKPAPTTGPSDSAAACRALGVLVCLGLTACGGGGNGRGGDTAAA
                     IECAPFARALTGVSLSGAAADWWREAGGQYARSHRPVVGSLLVLRRSSRLPSGHVAVV
                     SRVLSRREILVTQANWVHHRVSQDQPVEDVSPAGDWTRVRVWWPPADQMGIKEYAAFG
                     FIQPRGAFSHDQLISSTPSAIRIAEGER"
     gene            complement(1001..1675)
                     /locus_tag="VFN42_04475"
     CDS             complement(1001..1675)
                     /locus_tag="VFN42_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007281873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HET8995901.1"
                     /translation="MKKPAAPSARGLAVSVKTAKSRTAASQRWLARQLNDPYVAAAKQ
                     QGWRSRAAFKLLELDDRYHLIRPGMRVLDLGAAPGGWTQVAVKRGAASVLAVDLLPID
                     PVHGATMIQGDFTDPAMPAKLTGALGGAADLVLSDMAPNTTGHAATDHLRIVALAEMA
                     VEFAVGVLAPGGAFVAKVFQGGSERQVLDVLKRNFATVRHAKPPASRKESSELYVVAQ
                     GFRRPD"
     gene            complement(1672..2565)
                     /locus_tag="VFN42_04480"
     CDS             complement(1672..2565)
                     /locus_tag="VFN42_04480"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HET8995902.1"
                     /translation="MVRLGEGLHHTGRLSQAAMERALCALHACSARLGRRQVRRVRAV
                     ATEACRRAVNGTDFLRRVKDETGFEFDIRTSREEAELALESCTPLLPMDGRRALLFDI
                     GGGSTELAWVRLTCGRPELIGYDSLPVGVVTLAERWGDAGFSADGFEAMVADVVDRLQ
                     SFEKVHCIAREIRCGGVRLLGTSGTVTTLAGVALNLERYRRVAVDGVALPSTEAADAL
                     SHLRALGREGLLQHPCVGPERVEFVLPGCAVFAAITRVWPAPQVIVADRGLREGVLLR
                     LMRADRATSRYDARRRDVVRP"
     gene            2485..2799
                     /locus_tag="VFN42_04485"
     CDS             2485..2799
                     /locus_tag="VFN42_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995903.1"
                     /translation="MQRAQRPFHGRLAQPAGMMQPFTESNDAGETVEDPKPFARRRAN
                     KHSAIIRTQIKGGESRPENPAWLIGPRLLELRDRIKLSGCLNRYHPGIVIRLAITGKH
                     RI"
     gene            complement(2824..3732)
                     /locus_tag="VFN42_04490"
     CDS             complement(2824..3732)
                     /locus_tag="VFN42_04490"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET8995904.1"
                     /translation="MTCIEAYDSSIVAVLEEIRATATLEGLAVLDLAAEADESVVMYS
                     VGVAGPTTIATGRLLMTGQRGRPTHLVGQDGRPVLACPWVMAPGRPGGLLLWRSPRSP
                     GWTQTDYGLAASVALMVRMTIGTAAGYSGIDHLTGVPNRRWFLDEADRRAERLDHDGA
                     VASLLLIDVDNLKQLNASQGRELGDQLLVRVASQIRATVRPGDLVARVGPDEFAVWHD
                     GMDHLTAAERADALCNGRLFHGSPEHPRVSLSIGIVSRAPGASDDVRGLLRRAYMAAR
                     DVKARGGSGWRVAQPSSQPGCSDAAE"
     gene            3849..>4072
                     /locus_tag="VFN42_04495"
     CDS             3849..>4072
                     /locus_tag="VFN42_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HET8995905.1"
                     /translation="MRAVWYDRQGPADEVLVTGEIETPEPAAGQVLVRLEASGVNPSD
                     TYRRGGAVPAEYPRVIPNSDGAGTIDRLGDG"
BASE COUNT          707 a         1410 c         1308 g          647 t
ORIGIN      
        1 cggccggccg gaactcgccc gggattcgga ctacgcgacg aacgggcttc gctgccagaa
       61 ccgcgagacg ctggatacgg ccattgccgc ctggacgcgg acgatggctg cgaaagacgc
      121 cgaagcgatc cttgaggctg ccgaggttcc gtgctcccgc cttttcgata tcgcggattg
      181 cgctgcggac ccgcatttcc gcgcccgcaa ggcggtgcag gagatcgacg atccgctgat
      241 cgggcgcacg ctgcaccccg gcccgaccat tcgcctggat ggagaaccgc ctgagggtgt
      301 ggtccgctgg accggaccag ctgtgggcgc gcatacggat tatgtgctgc atacgcttct
      361 tggcctaccc aaggcgtcgg cctgaccggt cgtggttgat gtgtttccgc cggtgaaacc
      421 ggcaccaacc accgggccat cggacagcgc ggctgcctgc cgagcgcttg gggtgctggt
      481 atgcctgggc ctgaccgcct gtggcggtgg cggcaatggg cgtggcggcg acacggccgc
      541 tgccatcgaa tgcgcgccgt tcgcccgcgc gctgaccggc gtgtccctgt ccggagcagc
      601 cgccgactgg tggcgagagg caggcgggca gtatgcgcga tcgcaccgac ccgttgtcgg
      661 cagcctgctg gtcctgcgcc ggtcatcgcg gctgcccagc ggccatgtcg ccgttgtctc
      721 acgcgttctc agccggcgcg aaatcctggt gacacaggcg aactgggtgc atcatcgcgt
      781 cagccaggat cagcctgtcg aggatgtgtc accggcaggc gattggaccc gtgtccgggt
      841 ttggtggccg ccggcggacc agatgggcat caaggaatat gctgcgttcg gtttcatcca
      901 gccgcgcggg gcgttcagcc acgatcaact gatttcatcg accccctcag cgatccggat
      961 tgccgaaggc gagcggtaga agcggcaggc ggccgtagcg tcagtccggc cgccggaagc
     1021 cttgcgcgac gacgtacagc tcgctcgatt ccttccggct tgccggcggc ttggcgtggc
     1081 gcacggtggc gaaattgcgc ttcagcacgt ccagcacctg gcgctccgat ccgccctgaa
     1141 acacttttgc gacgaatgcg ccgcccgggg ccagcacgcc aacggcgaac tccaccgcca
     1201 tttcggccag cgcgacgatg cgcagatgat cggtcgccgc atggcccgtc gtattcggcg
     1261 ccatatccga cagcacgagg tcggccgccc cccccaaagc cccggtcagc ttcgccggca
     1321 tagcaggatc ggtaaagtca ccctgtatca tcgtggcgcc gtggaccggg tcgatcggca
     1381 gaagatcgac ggccaggacc gacgccgcgc cgcgtttcac ggcgacctgc gtccagcctc
     1441 ccggtgcggc accaaggtcg agcacccgca tccctggacg gatcagatgg tagcggtcat
     1501 ccagttccag cagtttgaag gcggcccgcg agcgccagcc ttgctgcttc gccgcggcca
     1561 cataggggtc gttcaactgt ctggcgagcc agcgttggga ggcggctgtg cgacttttcg
     1621 cggttttgac ggacacagcc aaaccgcgcg ccgatggcgc agcgggcttt ttcatggccg
     1681 gacgacgtcc ctgcggcgtg cgtcatagcg cgaggtcgcg cggtccgcgc gcatcagccg
     1741 cagcagcacg ccttcccgca gcccgcggtc tgccacgatc acctgcggag ccggccagac
     1801 ccgggtgatg gcggcgaata ccgcgcatcc aggcagcacg aattcgaccc gctccggccc
     1861 gacacacgga tgctgcaaaa gtccctcccg gcccagcgcg cgcagatgcg acagcgcatc
     1921 cgccgcctcg gtgctgggca gtgcgacgcc atcgaccgcg acgcggcgat aacgctccag
     1981 gttcagggcc acgccagcca aggtggtgac ggtgccgctg gtacccagca ggcgcacgcc
     2041 gccgcagcgg atttcccgcg cgatgcagtg caccttctca aacgactgca gccggtcaac
     2101 gacgtcggcg accatcgcct caaacccgtc cgcgctgaac ccggcatcgc cccagcgctc
     2161 cgccagcgtc accacaccca ccggcagcga gtcgtagccg atcagttccg gacggccgca
     2221 cgtcagccgc acccaggcga gttcggtgga gccgccgccg atatcgaaca gcaacgcccg
     2281 ccgtccatcc atcggcagca gcggcgtgca cgactccaac gccagttcgg cttcctcgcg
     2341 gctggttctg atgtcgaatt cgaacccggt ctcgtccttt acccggcgca ggaaatcggt
     2401 gccgttaacc gctcgacggc acgcttcggt ggcaacggcc cgcactcggc gcacctgccg
     2461 ccggcctaga cgtgcggagc aggcatgcag cgcgcacagc gcccgttcca tggccgcctg
     2521 gctcagcctg ccggtatgat gcagcccttc accgagtcga acgatgcggg agaaactgtc
     2581 gaggacccga aacccttcgc ccgaaggcgt gccaataagc attcggcaat tattcgtacc
     2641 cagatcaagg gcggcgaaag ccggcccgaa aacccggcgt ggctgatcgg gccgcgtctg
     2701 ctggaattgc gcgaccgtat caagctcagc gggtgcctga accggtatca tcctgggatt
     2761 gtcatccgac tggcgataac gggcaagcac cgaatttgat gagcctctga tctatcgctt
     2821 ttttcattcc gccgcgtcgg aacaacccgg ctgggaactg ggctgcgcga cgcgccagcc
     2881 actgccgcca cgggccttga cgtccctcgc cgccatgtag gcgcggcgca gcaggccgcg
     2941 cacgtcgtcc gaagcgcccg gcgctcggct gacgatgcca atcgacaggc tcacccgcgg
     3001 atgctcgggc gatccgtgga aaagccggcc attgcacagc gcatcggccc gttcggcggc
     3061 tgtcagatgg tccatgccgt cgtgccacac ggcgaattca tcggggccga ctcgcgcgac
     3121 cagatcgccc gggcggaccg tggcgcgaat ctggctggca acccgaacca gaagctggtc
     3181 cccaagctcc cgcccctggc tggcattcaa ctgcttcagg ttgtccacat caatcagcag
     3241 cagcgacgcc accgcgccat cgtgatccag cctctcggcc cggcggtccg cttcgtcgag
     3301 gaaccaacgg cgattgggca ccccggtcag atggtcgatg ccactgtagc cggccgcggt
     3361 tccgatcgtc atgcgcacca tcagcgcgac cgacgccgca agcccgtaat cggtctgcgt
     3421 ccagcccggc gaacgggggg agcgccacag cagcaacccg ccgggacgcc ccggcgccat
     3481 cacccacggg caggccagca ccggccggcc atcctgcccg accagatggg tgggacggcc
     3541 acgttggccg gtcatcagca ggcggccggt ggcgatggtt gtcggtccgg caacgccgac
     3601 gctatacatg acgacgcttt cgtccgcttc agccgcgaga tccagcacag ccagaccttc
     3661 cagggttgcg gtcgcgcgaa tttcctccag aaccgcaacg attgagctgt cataggcctc
     3721 gatgcacgtc acgcccaact ccttggagaa tacgccaaat atcttaagcg agacacatcg
     3781 atccagggta gaaattaacg acattgtaac aaccggcaat atcaggctgg ttccgggagg
     3841 aatgttcgat gcgcgctgtc tggtatgatc gccaaggtcc ggccgacgaa gtactggtta
     3901 ccggggagat cgaaaccccg gagcctgcgg ccggacaggt tctggtgcgg ctggaggcat
     3961 ccggcgtcaa tccctccgac acctaccgcc gcggcggagc cgtacccgct gaatatcccc
     4021 gggtgatccc gaacagcgat ggtgcaggca caatcgaccg gctgggcgat gg
//
LOCUS       DASSZH010000208         3234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1163998, whole genome shotgun sequence.
ACCESSION   DASSZH010000208 DASSZH010000000
VERSION     DASSZH010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163998"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..132
                     /locus_tag="VFN42_04500"
     CDS             7..132
                     /locus_tag="VFN42_04500"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HET8995906.1"
                     /translation="MLAFVNGMTHAELATHLDAPLGTVKSWIRRGLQALKACLEP"
     gene            129..815
                     /locus_tag="VFN42_04505"
     CDS             129..815
                     /locus_tag="VFN42_04505"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HET8995907.1"
                     /translation="MSDAPDDLDLLAAEYVLGTLDPAQARALAERADSDAAVRDAITA
                     GEERLAPLGRVAAPVAPPAELWQRVAASAGLRPRPVAAQPGWLARAWESLWLWRAATG
                     MGFALAAALAAVAWLGGAPARPVAALVPQGSAAAAYVAEMRPDGRLVLVALRQIPVAQ
                     GKVLELWALPKGATRPVSLGLLPSAGQSVRQSPARSPGTQLMISLEPAGGSPTGLPTG
                     PVLFAGTLGS"
     gene            complement(958..1296)
                     /locus_tag="VFN42_04510"
     CDS             complement(958..1296)
                     /locus_tag="VFN42_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET8995908.1"
                     /translation="MTRQSWAVRLSASAEADYDAILRWTMQRFGVAQAEVYARTLAAS
                     LARLERGPTIPGARRRDEIAAGLLTIHAGRRARHVILFRVAEAEDRAIDVLRILHEAM
                     DIGRHVPESE"
     gene            complement(1293..1580)
                     /locus_tag="VFN42_04515"
     CDS             complement(1293..1580)
                     /locus_tag="VFN42_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HET8995909.1"
                     /translation="MPTRNVVLTEQQTAMIDRLVRSGRYQNASEVLRDGLRLLQQQQA
                     EEEARLVALRQAARVGFEDFAAGRYRRFADGAALRAHLGARAAKLRRNSTE"
     gene            complement(1747..2487)
                     /locus_tag="VFN42_04520"
     CDS             complement(1747..2487)
                     /locus_tag="VFN42_04520"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8995910.1"
                     /translation="MHLHPDLASRVALVTGGSRGIGRAVAVALAEAGASVGVNYVARA
                     DEARAVAAQIAAAGGRAIAVGADVSDAASVDTMLARIAAELGPVDILVNNAGIAIPRG
                     LDDLTEEEFDRTIAVNLKSAFLCTRAVLPHMRAQRWGRIVNITSGAARGAGGVGVHYN
                     ASKAGMEGLTRGYAARLVKDGITVNAVAPSLIETDMLHGWAEEAAKRIPLGRLGAPEE
                     VAQAVLLVLGNAYMTGQTVILNGGMSFI"
     gene            complement(2508..>3234)
                     /locus_tag="VFN42_04525"
     CDS             complement(2508..>3234)
                     /locus_tag="VFN42_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET8995911.1"
                     /translation="LARRGMNAGSSGNVSLRSKHGMLITPSGCSADTLAADDVVAMDL
                     AGATQSAGTPSSEWAMHAAIYLDYPEAGCIVHTHADACTALACLNQPLPAFHYMVVRF
                     GGEDVRCAPYVTFGTPELARLAVQALAGRSACLLANHGMIVHGRDAEQALTGGVLLET
                     LCRQYLLARAAGPVRLLTAAEMQAAVERFKTYGGNPDSSPSPCGKGLGGGALEVTREA
                     LAGPLPPSPSRKGRGRSLSSNRS"
BASE COUNT          489 a         1146 c         1126 g          473 t
ORIGIN      
        1 atcatcgtgc tggccttcgt caacggcatg acccacgccg agctggcaac gcacctggac
       61 gcgccgctcg gcaccgtgaa atcctggatc aggcgcggat tgcaggcgct gaaagcgtgt
      121 ctcgaaccat gagcgacgcc ccggacgacc tggacctgct cgccgcggaa tacgtgctgg
      181 gcaccctcga tccggcgcag gcgcgcgcgc tcgccgagcg ggcggacagc gacgcggcgg
      241 tgcgggacgc gatcacggcg ggggaggagc ggctggcccc gctggggcgc gtggccgcgc
      301 cggtcgcgcc gccggcggaa ctctggcagc gcgtcgcggc gtcggccggg ttgcgtccgc
      361 ggcccgtggc ggcccaaccg ggatggctcg cccgcgcctg ggaaagcctg tggctgtggc
      421 gcgcggcaac ggggatgggg ttcgcgctgg ccgcagccct ggcggcggtg gcctggctgg
      481 gcggagcgcc cgcccggccg gtcgccgctc tggtgccgca gggcagcgcc gcggcggcct
      541 acgtggcgga gatgcggccc gacggccgcc tggtgctggt ggcgctgcgg cagatcccgg
      601 tggcgcaagg caaggtgctg gaactctggg cactgccgaa aggcgccacg cggcccgtgt
      661 cgctcggcct gttgccgtca gccggccaga gcgtccggca aagcccggcg cggtcaccgg
      721 gtacccagtt gatgatcagc ctggaacccg ccggcggctc accgaccggc ctgcccaccg
      781 gcccagtgct gttcgccggc acgctgggaa gctgaccgtt tcggcagatg ccagccacac
      841 ggacccgcac cgctcgccgc gatgggtagc agcgaggcgg agatggtggt ggaatttgcg
      901 aggctgtccg acaggggcgg accgacacgg tcctgatgcg acgctgccga gcggcggtca
      961 ttcactctct gggacatgcc gcccgatatc catcgcctcg tggagaatcc gcagcacgtc
     1021 gattgcccga tcctccgcct cggcaacccg gaacagaata acgtgccgtg cgcgtcgccc
     1081 ggcatggatc gtcagcaggc cggcggcaat ctcatctctc cggcgagcgc ccgggatcgt
     1141 cggcccacgt tccagccgcg ccagagaagc cgccaatgtc ctggcgtaga cctcagcctg
     1201 ggcgacgccg aaccgctgca tggtccaacg caggatcgcg tcatagtccg cctcggccga
     1261 agcggataac cgaaccgccc agctttgccg cgtcattcgg tggagttacg tcgcagctta
     1321 gcggcgcggg cgcccagatg cgcacgcaat gccgcgccat ctgcgaagcg acggtagcgt
     1381 ccggcggcga agtcctcgaa gccgacccga gccgcttgcc gcaatgccac aaggcgtgct
     1441 tcctcctcag cctgttgctg ctgcaacagg cgtagaccgt cgcgcagcac ctcgctggca
     1501 ttctggtacc ggccggaacg cacaagccga tcaatcatgg cggtttgttg ttcagtcaga
     1561 acaacgttgc gggtcggcat gcggcgctcc tttcgttcgg acgcgttggc agattatgcc
     1621 aaccactccg gcgtgcgcaa atggcgcatg ccaggaccgc gactgtgcct ccgccgatcg
     1681 ctggcacggc ttgatgaaga agggactatg aggaagcggc ttgcaaaccc ctgacccgcg
     1741 ccgcggtcag atgaagctca tcccgccgtt caggatcacc gtctgcccgg tcatataggc
     1801 gttgcccagc accagcagca ctgcctgggc gacttcctcc ggcgcgccga gccggcccag
     1861 cgggatgcgc ttcgccgcct cctcggccca gccgtgcagc atgtcggtct cgatcagcga
     1921 cggggcgacg gcgttcaccg tgatgccgtc cttcaccagg cgcgcggcat agccgcgggt
     1981 cagcccctcc atgccggctt tggaagcgtt gtagtgcacg ccgactccgc cggcgccgcg
     2041 ggccgcgccg gaggtgatgt tgacgatgcg gccccagcgc tgcgcgcgca tatgcggcag
     2101 cacggcgcgg gtgcacagga aggcggattt caggttcacc gcgatggtgc ggtcgaattc
     2161 ttcctcggtc aggtcgtcca ggccacgggg aatcgcgatg ccggcgttgt tcaccaggat
     2221 atccaccggg ccgagttccg ccgcgatgcg cgccagcatg gtgtcgaccg aagcggcatc
     2281 ggacacgtcc gcccccaccg cgatcgctcg gccgccggcg gcggcgatct gtgccgccac
     2341 agcccgggcc tcatcggccc gggcgacgta gttcaccccc accgacgcac ccgcttccgc
     2401 cagcgccacc gccacagcgc ggccgatgcc gcgtgagccg ccggtcacca gggcgacgcg
     2461 agaggccaag tctggatgga ggtgcatgcg ggcgcctttc gcgatggtca ggaacggttg
     2521 ctggacagac tcctccccct ccccttgcgg gagggggagg gggggagggg gccggcaagg
     2581 gcttcccggg taacctcaag cgcccctccc cccaacccct tcccgcaagg ggagggggag
     2641 gagtccggat tcccaccgta tgtcttgaac cgctccaccg ccgcctgcat ttccgcggcg
     2701 gtaagcagcc gcaccgggcc ggcggcgcgg gccagcaggt attgccggca cagcgtttcc
     2761 agcagcaccc caccggtcag cgcctgctcc gcatcgcgcc cgtgtacgat catgccgtgg
     2821 ttcgccagca ggcaggcgct gcgtccggct agcgcctgca ccgccagtcg cgccagttcg
     2881 ggcgtgccga acgtcacgta gggcgcgcag cgcacgtcct cgccgccgaa gcgcaccacc
     2941 atgtagtgga aggccggcag cggctggttg aggcaggcca gcgcggtgca ggcatcggca
     3001 tgggtgtgca cgatgcagcc ggcctccgga taatccaggt agatcgccgc atgcatcgcc
     3061 cattcggaag acggcgtgcc ggcgctctgc gtcgcaccgg caaggtccat cgccaccacg
     3121 tcatccgccg ccagcgtatc ggcggagcag ccggacggcg tgatcagcat gccgtgcttg
     3181 ctgcgcaggc tgacattgcc ggagctgccg gcattcatcc cgcgccgcgc cagc
//
LOCUS       DASSZH010000209         3034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1675279, whole genome shotgun sequence.
ACCESSION   DASSZH010000209 DASSZH010000000
VERSION     DASSZH010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1675279"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VFN42_04530"
     CDS             <1..215
                     /locus_tag="VFN42_04530"
                     /EC_number="4.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942367.1"
                     /note="With PurE catalyzes the conversion of
                     aminoimidazole ribonucleotide to carboxyaminoimidazole
                     ribonucleotide in the de novo purine nucleotide
                     biosynthetic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HET8995912.1"
                     /translation="VVKNLVGPEETALWPRIVATPGLIPHLYGKRDARPGRKMGHVTR
                     LFPRGSLPGEFGVQAALGPVGVVPSF"
     gene            complement(212..967)
                     /locus_tag="VFN42_04535"
     CDS             complement(212..967)
                     /locus_tag="VFN42_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8995913.1"
                     /translation="MARDRVVLVTGAASGIGAAVCRALAGPNRAILVHTRRNQAGAEA
                     VVWGIRAAGARAEIALGDLADPAVAERLVAEAVARFGALDVLVSNAGFADRTAFASLS
                     DAAMAHSGEAIQGAFFRLARAAIPHLRASEHPRVVAVSSFVAHVFRPDLTLFPASAAA
                     KAAVEALVRALALELAPDGVTVNAVVPGFIRKDAGAHAALEPEALRRQTACIPLGRLG
                     SPEEVAAAVAFLASPAASYITGQAVHVSGGLVV"
     gene            complement(1002..1886)
                     /locus_tag="VFN42_04540"
     CDS             complement(1002..1886)
                     /locus_tag="VFN42_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET8995914.1"
                     /translation="MQPPTLRQLRTFLAVVETGSVSAAAQSLNLTQPAASQQLRELER
                     ALGVRLLHRAAGRMLPTPAGEAVIEPARRIQGALDDTIAAVQAFRSGAVGRVRLGTGA
                     TACINLLPPVLAAVKQRMPGLEIAVATGNSNEIAHRVAVGELDIGLVTLPLQPNRALT
                     ATRLLNDPLLAVIPRAHAPDGDSISAARLAALPLILYEPGGSTRAIIDAWFRRAGPPP
                     QPAMELDSIETIKVLVGGGLGASILPQLALGSPVRDAVVKPLRPALGRQLGIVLRREK
                     VLDRGLRLVLDALRETAG"
     gene            1983..2528
                     /locus_tag="VFN42_04545"
     CDS             1983..2528
                     /locus_tag="VFN42_04545"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8995915.1"
                     /translation="MTAAACITIRPSQRDDVPGFQAIYAHHVLHGTASFETEPPDVAE
                     MHQRRATVLSKGFPYLAAECDGAVIGYAYASTYRPRAAYGDTVENSIYLRPDVTGRGF
                     GRPLLEALIAACEQCDLRQMIAVVGDSAHVASVRLHQRCGFHLVGVLRDVGWKHGRWL
                     DSVLLQRSLGRGNTVPPMERR"
     gene            2528..>3034
                     /locus_tag="VFN42_04550"
     CDS             2528..>3034
                     /locus_tag="VFN42_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8995916.1"
                     /translation="MRHRRRLAVVLGINQTMSWGVTFYLPAIIADPAAHTLHASPMAV
                     LGAFSWALLVTGFCAPRVGRWIDTNGGRGVLSLSVLVLAAGLVLLAAAPGPTMWYAAW
                     SVLGVGMALGLYDAAFATVGTLLGREAGPIITGITLIAGLASTVFWSLGAGLIGMLGW
                     RGLLLFYAG"
BASE COUNT          471 a         1077 c         1012 g          474 t
ORIGIN      
        1 cggtggtgaa gaacctcgtg ggtccagagg aaaccgcgct ctggccgcga atcgtcgcca
       61 cgcccgggct gattccgcat ctttatggca agcgcgacgc gcgtcccggc cggaagatgg
      121 gccatgtgac acggctgttt ccacgcggct cgctgcccgg ggagttcggc gtgcaggcgg
      181 cgctgggacc agtaggcgtc gtgccgtcct tctagacgac caatccgccg ctgacatgca
      241 cagcctggcc cgtgatgtag cttgccgcgg gtgaggccag gaaggcgaca gcggcagcaa
      301 cctcctcggg cgatcccagc cggccgagcg gaatacatgc ggtctgccgg cgcagcgcct
      361 ccggctccaa ggcggcatgt gcacccgcgt ccttgcggat gaagccgggc accaccgcat
      421 tgaccgttac cccgtccggc gccagttcca gcgccagcgc ccggaccaac gcctccacgg
      481 ccgccttcgc cgcggcggag gcgggaaaca gcgtcagatc cgggcggaac acgtgggcga
      541 caaaactgga caccgcaacc acgcgcggat gttccgacgc ccgcagatgc gggatggccg
      601 cgcgggcgag ccggaaaaac gcgccctgga tcgcctcccc cgaatgcgcc atggccgcgt
      661 cgctcagcga ggcgaaggcg gttcggtcgg caaacccggc attgctcacc aacacatcca
      721 gtgcgccgaa gcgcgccaca gcctccgcaa ccagccgttc ggccacggcc ggatcggcca
      781 gatccccaag agcgatctca gcccgggcgc cagctgcacg gattccccac acgaccgcct
      841 ccgctcccgc ctgattgcgc cgcgtatgca cgagtatggc gcggttcgga ccggccagtg
      901 cccggcacac cgcggcgccg atgccgctcg ccgcacccgt gaccagaacc acccgatcgc
      961 gcgccatcct tcccctccac cttcgtatga ctctcgccgg ctcagcctgc cgtttcccgc
     1021 aatgcgtcca gcacgaggcg caggccgcgg tccagcacct tctcgcgccg cagcacgatg
     1081 ccgagctgcc tgcctagggc cggccgcaac ggcttcacca ccgcatcgcg caccggactg
     1141 cccagggcga gttgtggtag aatggaagct ccgagtccgc caccgaccag caccttgatc
     1201 gtctcgatgc tgtccagctc catcgccggc tgcggtggag ggccggcgcg gcgaaaccaa
     1261 gcgtcgatga tcgcacgggt gctgccgccg ggctcgtaga ggatcagtgg caaagccgcg
     1321 agccgggcgg ccgagatcga gtctccgtcc ggcgcgtgcg cgcgcgggat gactgccagc
     1381 agcgggtcgt tcagcagccg cgtggccgtc agcgcccggt taggctgcaa cggcagcgtg
     1441 accaggccga tatccagctc ccccaccgcg acgcggtgcg cgatttcgtt gctgttgccg
     1501 gtggcgacgg cgatttccag ccccggcatg cgctgcttca ccgccgccag gaccggcggc
     1561 agaaggttga tgcaggcggt cgcgcctgtc cccagccgca cccggccgac cgcgccggaa
     1621 cgaaatgcct gcacggcggc gatcgtatca tccagcgcgc cctgaatgcg gcgcgccggc
     1681 tcgatcaccg cctcaccggc cggagtcggc aacatgcggc cggccgcgcg gtgcagcaga
     1741 cgcaccccaa gcgcacgctc cagctcgcgc aattgctggc tggcggccgg ttgcgtcagg
     1801 ttgagagact gggctgccgc ggagacggag ccggtctcga cgactgcgag aaaggtccgt
     1861 agctggcgca gggtcggggg ctgcataagc gaaacctatg ctgatacgct gcatcacaag
     1921 attttccttc gcggctgcgg ggtcaagccg catgcttccg aagcccgttt gaggaacagc
     1981 cagtgaccgc cgcagcttgc atcaccatcc ggccgagtca gcgcgacgac gtcccaggat
     2041 tccaggcgat ctacgcgcac cacgtgctgc acggcaccgc atcattcgaa accgaaccac
     2101 cggacgtcgc ggaaatgcac cagcgccgcg ccacggtgtt gagcaagggt tttccttatc
     2161 tcgcggccga gtgcgatggc gcggtgattg gctacgccta cgccagcacc tatcgtccgc
     2221 gcgcggccta tggtgacacc gtggagaact cgatctacct gcgccccgac gtcaccggcc
     2281 gcggcttcgg ccggcccctg ctcgaggcac tgatcgctgc ctgcgagcaa tgtgacctgc
     2341 ggcagatgat cgcggtggtg ggggacagtg cacacgtcgc ctccgtccga ttgcatcagc
     2401 gctgcggctt ccaccttgtc ggcgtcctgc gggatgtcgg gtggaaacac gggcgctggc
     2461 tcgattcggt gctgctgcaa cgcagccttg gccgtggcaa cacggtccct ccgatggagc
     2521 gccgctgatg cgccaccgcc gccggctcgc cgtcgtgctc ggcatcaatc agaccatgtc
     2581 gtggggcgtg accttctacc tgccggcgat catcgccgat cccgccgcgc acacgttgca
     2641 cgcatcgccc atggcggtgc tgggtgcctt ctcctgggca ctgctggtca ccggcttctg
     2701 cgccccccgc gtgggacgct ggatcgacac caatggcggc cggggtgtgc tgagcctcag
     2761 cgtactggtg cttgcagccg gactcgtgct gctggcagcc gcgccggggc ccacgatgtg
     2821 gtacgcagcc tggagcgtgc tgggcgtcgg catggccttg gggctgtacg acgccgcgtt
     2881 tgccaccgtc ggcacgttgc tggggcgtga ggcagggccg atcatcaccg gcatcaccct
     2941 gatcgccgga ttggccagca ccgttttctg gtcgctcggg gctgggctga tcggcatgtt
     3001 gggttggcgc ggcctgctgc tgttctatgc cggg
//
LOCUS       DASSZH010000210         3809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1607243, whole genome shotgun sequence.
ACCESSION   DASSZH010000210 DASSZH010000000
VERSION     DASSZH010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607243"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="VFN42_04555"
     CDS             <1..852
                     /locus_tag="VFN42_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8995917.1"
                     /translation="NIVDVLFDSAGMVDPAGKLQIEKDLGLDKPVVVQYADWIDGLAH
                     GNLGYSYIDEKPVLSEIAPRIPVTAELGLLALGFSIVFGVPLGVISAVRQNSRLDYLL
                     RVVSLSGLSIPAFWLGLLILMAMVAWLGDLPTYTDLPHAFWPRLGLLAIPAAAVGFRS
                     SALIMRLTRSSMLEVMRQDYIRTARAKGATAQSVNYNHALRNALLPVTTVIGIEAAFL
                     IGGLIVTETVFNIPGMARFLVESIRVRDYPVVQNLVMVIAVVVVLINFVVDLLYAVID
                     PRIKIAD"
     gene            856..1752
                     /locus_tag="VFN42_04560"
     CDS             856..1752
                     /locus_tag="VFN42_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8995918.1"
                     /translation="MSASAEFESELARAGARTGTLWGKTVFFARQYPLGAIGAAIMIV
                     FIGAAVFAGFITRWSPLTTNPALSLAAPSAAHWFGSDFMGRDIFSRIVYGARISLAVG
                     LGSTGLGCLLGVILGLLSGYVGGWLDLAVQRIIDMLQALPLLVLALVMAAALGPSLQN
                     TIISISIPLVPYVARVIRSNTLALRELSFVEAARAIGMSEFRIAVRHILPNTIAPLIV
                     LGTAQLGSAILTEASLSFLGLGVPEPYPSWGRMLSASAAEYVRTAPWLVIFPGIAISI
                     VVFGTNLLGDALRDILDPRQRG"
     gene            1752..2780
                     /locus_tag="VFN42_04565"
     CDS             1752..2780
                     /locus_tag="VFN42_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8995919.1"
                     /translation="MSDQPQAAAPVLEVDGLRTVFFTRAGLLEAVHNVSFSVGRGDVL
                     AVVGESGCGKSMTALSLMRLVPSPPGRIVAGSVRLDGRDLLKLDESEMRRVRGNDISM
                     IFQEPMTSLNPVVTIGKQISEAIRLHQDLSRSAAMDKAIEMLRLVRIPEPKQRAREYP
                     HQLSGGMRQRAMIAMAVACNPKVLIADEPTTALDVTIQAQILDLLLQLQQELGTAIIM
                     ITHDLGVVAEMAHRVLVMYAGRKIEEAPVAELFARPLHPYTHGLLASVPRLASMRDDA
                     DPAEERLQDIPGMVPPLTHLPQGCAFAPRCKFADDTCRAAYPDYVARRAGHFVACWHA
                     DKLYGAAS"
     gene            2777..3751
                     /locus_tag="VFN42_04570"
     CDS             2777..3751
                     /locus_tag="VFN42_04570"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HET8995920.1"
                     /translation="MTVPVIEVDGLKKHFPIRRGLLRKTVGQVYAVDGVSFSVAEGET
                     LGLVGESGCGKSTVARTVLRLIEPSAGSIRLDGKDITGLSKPELRPYRRQMQMIFQDP
                     FSSLNPRMKAAAIVREPLAVHGVASGKAADDRVAELFELVGLRAEQMDNFPHQFSGGQ
                     RQRISIARALALKPRLIVGDEPVSALDVSIQAQVINLLMDLQQKQKLSYLFIAHDLAV
                     VEHISHWVAVMYLGKIVEYADKRTLFRSPQHPYTEALLSAVPVPDPALKRRKRLLEGD
                     VPSPVNPPPGCAFHTRCPYAEARCRQEAPALREIAPGHSVSCHLRTGQ"
BASE COUNT          572 a         1314 c         1233 g          690 t
ORIGIN      
        1 aatatcgttg atgtcctgtt cgactccgcc ggcatggtcg atccggcggg caagctgcag
       61 atcgagaagg acctcggcct cgacaagccg gttgtcgtgc aatacgccga ttggatcgat
      121 ggcctggccc acggcaacct cggctattcc tacattgacg agaagcccgt gctcagcgag
      181 atcgcgccgc gcatcccggt caccgccgaa ctcggcttgc ttgcgcttgg cttctccatc
      241 gtgttcggcg tgccgctggg cgtcatcagt gcggtcaggc agaattccag gctggactac
      301 ctgctccgtg tggtcagtct cagcggcctg tcgatccccg ccttctggct cggtttgctg
      361 atcctgatgg cgatggtcgc ctggctgggc gacctgccca cctacaccga cctgccgcac
      421 gcattctggc cgcggcttgg cctgctggcg atccccgccg ccgccgtcgg cttccgcagt
      481 tcggcactga tcatgcgcct gacgcgatcc tccatgctgg aggtgatgcg gcaggactac
      541 atccgcaccg cccgcgccaa gggcgccacc gcgcagagcg tcaactacaa ccacgcgctg
      601 cgcaacgcgc tgctgccggt caccaccgtc atcggcatcg aggccgcgtt cctgatcggc
      661 ggcctgatcg tcaccgaaac cgtgttcaac atccccggca tggcgcgctt cctggtcgaa
      721 tccatccggg tgcgcgatta cccggtggtg cagaacctgg tgatggtcat cgccgtcgtc
      781 gtcgtcctca tcaacttcgt ggtcgacctg ctgtacgcgg tgatcgatcc gcgcatcaag
      841 attgctgact gagccatgtc cgcttccgca gaattcgaat ccgagctggc gcgcgccggc
      901 gcgcgcaccg gcacactctg gggcaagacc gtgttcttcg cccgccagta tccgctgggc
      961 gcgatcggcg ccgcgatcat gatcgtgttc atcggcgcgg cggtcttcgc cggcttcatc
     1021 acgcgctggt cgccgctcac caccaacccg gcgttgtcac tcgccgctcc cagcgcggcg
     1081 cactggttcg gctccgactt catgggccgc gacatcttca gccgcatcgt ctatggcgcg
     1141 cgcatttcgc tggcggtggg gcttggctcc accggcctgg gctgcctgct gggggtgatc
     1201 ctgggcctgc tgtccggcta tgtcggcggc tggctggacc tggctgtcca gcgcatcatc
     1261 gacatgctgc aggcactgcc gctgctggtg ctggccctgg tgatggctgc ggcactcggg
     1321 ccatcgctgc agaacaccat catctccatc tccatcccgc tggtgcccta cgtcgcccgc
     1381 gtcatccgct ccaacacgct ggcattgcgg gaactgtcct tcgtcgaagc ggcgcgtgcc
     1441 atcggcatga gcgagttccg catcgccgtg cgccacatcc tgcccaacac catcgcgccg
     1501 ctgatcgtgc ttggcacggc gcagctcggt tcggcgatcc tgaccgaggc atcgctgtcc
     1561 ttcctcggcc tgggtgtgcc ggagccgtac ccctcctggg gccgcatgct ctccgcctcg
     1621 gccgccgaat atgtgcgcac cgcgccctgg ctggtgatct tccccggcat cgccatcagc
     1681 atcgtcgtgt tcggcaccaa cctgctcggc gacgccctgc gcgacatcct cgacccgcgg
     1741 cagcgcggct gatgagcgat caaccgcagg cggcggcgcc ggtgctggag gtggacgggc
     1801 tgcgcacggt gttcttcacc cgggccgggc tgctggaagc cgtccacaac gtgtccttct
     1861 cggtcggccg cggcgacgtg ctcgccgtgg tcggcgagtc cggctgcggc aagtcgatga
     1921 ccgcgctgtc gctgatgcgg ctggtgccaa gcccgcccgg acgcatcgtc gccgggtcgg
     1981 tgcggctgga cggccgcgac ctgctgaaac tggacgagtc ggagatgcgc cgcgtgcgcg
     2041 gcaacgacat ctccatgatc ttccaggagc cgatgaccag cctcaatccg gtggtgacca
     2101 tcggcaagca gatcagcgag gcgatccggc tgcaccagga cctgtctcgc agcgcggcga
     2161 tggacaaggc gatcgagatg ctgcgcctgg tgcgcattcc cgaacccaag caacgtgccc
     2221 gcgaatatcc gcaccagctc tccggcggca tgcgccagcg cgcgatgatc gccatggcgg
     2281 tggcctgcaa tcccaaggtg ctgatcgccg acgagccgac cacggcgctc gacgtgacca
     2341 tccaggcgca gatcctcgac ctgctgctgc aactgcagca ggaattgggg acggcgatca
     2401 tcatgatcac ccatgatctg ggcgtggtcg cggaaatggc gcatcgcgtg ctggtcatgt
     2461 atgcaggccg caagatcgag gaagcgccgg tcgcggagct gttcgcccgc ccgctgcatc
     2521 cctacacgca cgggctgctt gcctcggtgc cgcggcttgc cagcatgcgc gacgacgcgg
     2581 atccggccga ggaacgcttg caggacattc ccggcatggt gccgccgctg acccatctgc
     2641 cgcagggctg cgccttcgca ccgcgctgca agttcgccga cgatacctgc cgtgcggcct
     2701 atccggacta tgtcgcacgc cgcgcgggac atttcgtcgc ctgctggcac gccgacaagc
     2761 tctacggagc cgcgtcatga ccgtgccggt gatcgaagtt gacggcctga agaagcactt
     2821 tccgatccgc cgcgggctgc tgcgcaagac cgtcggccag gtctatgcgg tggacggtgt
     2881 cagcttctcg gtggcggagg gcgaaacgct gggcctggtc ggtgaatccg gctgcggcaa
     2941 gtccaccgtc gcccgtaccg tgttgcggct gatcgagccc tctgccggta gcatccggct
     3001 ggacggcaag gacatcaccg gcctgtccaa gccggaacta cggccgtacc gccggcagat
     3061 gcagatgatc ttccaggacc cgttctcctc gctcaatccg cgcatgaagg cggcggcgat
     3121 cgtgcgggag ccgctggcgg tgcatggtgt cgcgtccggc aaggcggccg atgaccgtgt
     3181 cgccgaactg ttcgagctgg ttggcctgcg cgccgagcag atggacaatt tcccgcatca
     3241 gttcagcggc gggcagcggc agcgcatcag catcgcgcgc gcactggcgc tgaagccgcg
     3301 gctgatcgtc ggcgatgagc cggtgtcggc gcttgacgtc tccatccagg cgcaggtcat
     3361 caacctgttg atggatctgc agcagaagca gaagctgtcc tatctgttca tcgcgcacga
     3421 cctggcggtg gtggagcata tcagccactg ggtcgcggtg atgtacctcg gcaagatcgt
     3481 cgaatacgcc gacaagcgca cgctgttccg cagcccgcag catccctaca ccgaagcgct
     3541 gctctcggcc gtgccggtgc cggatccggc gttgaagcgc cgcaagcggc tgctggaagg
     3601 cgacgtgccg agcccggtga atccgccgcc cggatgcgcc ttccatacgc gctgtcccta
     3661 tgcggaggcg cgctgccggc aggaggcgcc ggcgcttcgg gagatcgcgc cagggcattc
     3721 cgtgtcctgt catctgcgga cagggcaata gccacgcagt cgccggttca ggcctgcaac
     3781 caaggctcga agctctgtga gtccgcccg
//
LOCUS       DASSZH010000211         3841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1470731, whole genome shotgun sequence.
ACCESSION   DASSZH010000211 DASSZH010000000
VERSION     DASSZH010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470731"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VFN42_04575"
     CDS             <1..213
                     /locus_tag="VFN42_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET8995921.1"
                     /translation="ALNANYGLLWWLNTGCTRYPSAPAASFFASGAGGNITFVSPEHD
                     IVAIMRWMDRDSTDQFLRMMLAALRH"
     gene            complement(239..928)
                     /locus_tag="VFN42_04580"
     CDS             complement(239..928)
                     /locus_tag="VFN42_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8995922.1"
                     /translation="MHDIDEALCVLVADQQELFRRGVIGVLRDSWPLWSCAHASGIEE
                     IRHLLAREAANLLIVELALPGLGGPAGIQQLRALHPHCTVVVLGGSDDRATVLESLGA
                     GAQGYVAKSATPTQLLRAVEAVLCGAVVAPASLAGRHLPAEAPRPPSRQAPAAAPPGF
                     TGRQADVFRLLAEGCATKTIARRLNLAAGTVKVHLAAIYRQLGVHGRVEALARFRDIA
                     IADLVSGEAGD"
     gene            complement(945..1127)
                     /locus_tag="VFN42_04585"
     CDS             complement(945..1127)
                     /locus_tag="VFN42_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995923.1"
                     /translation="MPSAELEGDLSAWRNLLWDSVRMLQAGRPDLARRFREAANDIER
                     NLQAARQMPRRGLAAT"
     gene            complement(1139..1573)
                     /locus_tag="VFN42_04590"
     CDS             complement(1139..1573)
                     /locus_tag="VFN42_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995924.1"
                     /translation="MHADPAVFVRKPLGLAFDIAELLLAQAFAASCGSRLVVETNHHA
                     GNDEFEEVLALYAADRRACRCLIWRSEAAVVVQPMPGRPQRFASLGEALDWLAPEGRV
                     AVTDLTLGTNPGRAFSAPPAEPPDQARTQAVGGGWQLRPSRA"
     gene            1876..2172
                     /locus_tag="VFN42_04595"
     CDS             1876..2172
                     /locus_tag="VFN42_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995925.1"
                     /translation="MNTLSLFGGMLAVMLLLAPGLAAAQNGGATANRPGYARGNANAP
                     TGMTGNYRGWDSARGSGSGANNFVGNGRGQPGTGNPNAGRFGGWFANNHRGQRD"
     gene            complement(2195..3145)
                     /locus_tag="VFN42_04600"
     CDS             complement(2195..3145)
                     /locus_tag="VFN42_04600"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8995926.1"
                     /translation="MNPSRRACLAASLAGMLVATVAGFAGGARLVTFGAVAAMHICKP
                     FQALNLIAPRAGIVATTGIAYAPGPRHKLDLYAPARSARPAPVVVFFYGGGWETGDRA
                     TYRFVGASLAERGVVVAIPDYRLHPAVRFPAFEQDAAAAIAWVKANIGRHGGDARRLF
                     LMGHSAGAQIAALLAVDPEYLWAQHLSPRDVCGVIGLAGPYDFRPASKQGQDLFGPPQ
                     AWARWQPITYASAAAPSMLLLAGASDRTIDPRSTLRFAASLQAKGGSAESRLFPGITH
                     TAIVAALATPLHFLAPAADATLALIRRGGSCGPASAGETR"
     gene            3228..>3841
                     /locus_tag="VFN42_04605"
     CDS             3228..>3841
                     /locus_tag="VFN42_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET8995927.1"
                     /translation="MLHPHPFSLFPNPRVGTPGLTVLPAAGFRRAKAEIATWPGYQPT
                     PLRDLPRLAAGAGLAGLRLKDESGRFGLGSFKALGGAYAVAMLLLNELARTGVAAAAS
                     TAELTAGTHRAATQGMTVTCATDGNHGRAVAWGAQRFGARCVIFVHATVSQNRVDAIA
                     RYGAEVRRVPGTYDDAVREAARQAEANGWFVVSDTSWPGYTEVPR"
BASE COUNT          621 a         1331 c         1298 g          591 t
ORIGIN      
        1 gcgctgaacg ccaattacgg cctgctgtgg tggctgaaca ccggctgcac gcgctatccc
       61 agcgccccgg cagccagctt cttcgcctcc ggcgcgggcg gcaacatcac cttcgtgtcg
      121 ccggagcatg acatcgtcgc catcatgcgc tggatggatc gcgacagcac cgaccagttc
      181 ctgcgcatga tgctggctgc attgcggcac tgacgcggcg agcgcggcat cgacagcgtc
      241 agtcgccggc ttcgcctgac accaggtccg caatggcgat gtcgcggaac cgcgccaagg
      301 cctccacccg cccgtgcacg cccagttgcc ggtagatcgc cgccaggtgc accttcacgg
      361 tgccggctgc caggttcagc cggcgggcaa tggtcttggt ggcgcagcct tccgcgagca
      421 gccggaacac gtccgcctgc cgaccggtaa atccgggtgg tgcggcagcc ggggcttgtc
      481 gggatggcgg ccgcggcgct tccgccggga ggtgccggcc agccaggctg gccggagcca
      541 ccacggcgcc gcacagcact gcctccacgg cgcgtagcag ttgggtcggc gtggcggatt
      601 tcgccacata gccttgcgcg cccgcgccaa ggctttccag gaccgtggca cgatcgtcgc
      661 ttccgcccag caccaccacc gtgcaatgcg ggtgcaacgc gcgaagctgc tggatgccgg
      721 ccggaccgcc caatccgggc agtgcaagtt ccacgatcag caggttggcg gcttcccgag
      781 ccagcaggtg cctgatttcc tcaatgccgc tggcatgcgc gcatgaccac agcggccagg
      841 agtctcgcag cacgccgatg acgccgcggc ggaacagctc ttgctggtcg gccaccagca
      901 cgcagagtgc ttcgtcgatg tcgtgcatcc tgcctattcc gctctcaggt tgctgccagg
      961 ccgcgacgcg gcatctgccg cgcggcttgc aggttgcgct cgatgtcgtt cgcggcctcg
     1021 cggaaccgcc gtgccaggtc gggccgtccg gcctgcagca tgcgtaccga atcccacagc
     1081 agattgcgcc atgcgctgag atcgccctcc agttcggcgg acggcattac gcggctgctc
     1141 atgcccggga cggacgcaac tgccatccgc ctcccacagc ctgcgttctc gcctggtccg
     1201 gcggttctgc ggggggcgcg ctgaatgcgc gtccgggatt cgtgccgagc gtaagatcgg
     1261 tgactgcgac ccgcccttcc ggcgccagcc aatccaacgc ttcgccgagt gacgcaaacc
     1321 gttgcggccg gccgggcatg ggctgcacca ccacagcggc ctcgctgcgc cagatcagac
     1381 aacggcaggc gcgccggtcg gcggcataca gcgccagcac ttcctcgaat tcgtcgttac
     1441 cggcgtggtg attggtttcc acgaccaagc ggctgccgca ggaggcagcg aaggcctggg
     1501 cgagcagcag ttccgcaatg tcaaaagcca gcccgagcgg tttccggaca aagacggcag
     1561 ggtcagcgtg catgatgatg gggactcgcg gggttgatca cgtctgcgtg actaaagccc
     1621 atcacgcggg cgtgaggcca ttcacaaacg tgattttccg tggcgggtgc gatccgagtg
     1681 cggagcggaa cgaaaaaccc gctgaaccaa gtcctgcaag catctgaaaa agcatgaaat
     1741 taatcgaagc tttcacggaa catttactgg ctaattcgtt ccgggaagtt gcctctggcc
     1801 tggccatccg aaccaatccg ccgggcgcgc gttgctggac ggactcagaa ccccatggcg
     1861 caaggagcgg acaggatgaa cacgctctcc cttttcggcg gcatgcttgc cgtcatgctg
     1921 ctgctcgcgc ctggtctggc ggcggcgcag aatggcggtg caaccgccaa ccggcccggc
     1981 tacgcgcgcg gaaatgcgaa tgcgccgact ggcatgacag gcaattaccg cggctgggac
     2041 agcgcgcggg gcagcggcag cggcgcgaac aactttgtcg gcaacgggcg cggacagccg
     2101 ggtaccggta atccgaatgc cggccggttc ggcggctggt ttgccaacaa ccaccgcggg
     2161 cagcgggact gatccaccag gctgcgatcc ggcattatcg cgtttcgccg gcacttgccg
     2221 ggccgcagct tccgccgcgc cggatgaggg cgagcgttgc gtccgccgcc ggcgccagaa
     2281 agtgcagtgg cgtggcaagt gcggcgacga tcgcggtgtg ggtgatgccc ggaaagaggc
     2341 ggctttccgc cgatccgccc ttggcctgca agctggccgc gaaacgcagg gtgctgcgcg
     2401 gatcgatcgt ccggtcggac gctccggcca gcagcagcat cgatggagcg gccgctgatg
     2461 cgtaggtgat cggctgccag cgtgcccatg cctggggcgg gccgaagagg tcctgcccct
     2521 gtttgctcgc cgggcggaaa tcgtacggcc ccgccaggcc aatcacgccg cacacgtcgc
     2581 gtggcgacag atgctgcgcc cacaggtatt ccggatcgac tgccagcaaa gcagcgatct
     2641 gcgccccggc cgaatggccc atcaggaaca gccgccgcgc atccccgcca tggcggccga
     2701 tattggcctt cacccaggcg atcgccgctg cggcgtcttg ttcgaaagcg ggaaaccgca
     2761 cggccggatg caggcggtaa tccggaatcg ccaccacgac gccgcgctcc gccaggctgg
     2821 cgccgacgaa gcggtatgtg gccctgtcgc cggtctccca gccgccgcca tagaagaata
     2881 ccacgacggg cgccggcctg gcgctgcgcg ccggggcata gagatccagc ttgtggcgcg
     2941 gcccgggcgc ataggcgatg ccggtcgtcg cgacgatccc ggcacgcggc gctatcagat
     3001 tcagcgcctg gaacggtttg cagatgtgca tggccgccac ggcaccgaag gtcacgaggc
     3061 gcgcgccacc ggcaaagccg gcgaccgtgg cgaccagcat tccggcgaga ctggcggcca
     3121 ggcaagcgcg gcgtgacggg ttcatgtcca ggattacagg ccatgcaggt tagcaaagcc
     3181 tcaacgggcg ggtcgccttg gacctccggc gcgcctatcc tgcggccatg ctgcatcctc
     3241 acccgttcag cctgttcccc aatccgcgtg tcggtacccc tggactgacc gtgctgcccg
     3301 ctgccggatt ccgccgagcg aaggcggaga ttgcgacctg gcctggttac cagccgacac
     3361 ccctgcgcga cctgccgcgc ctggctgccg gcgccggact cgccgggctg cggctgaagg
     3421 atgagtccgg ccgctttggc ctcggctcgt tcaaggcact cggcggcgcc tatgcggtgg
     3481 cgatgctgct gctgaacgag ttggcgcgca ccggcgtcgc cgccgcggcc tccaccgcgg
     3541 aactcacggc cggcacccac cgcgccgcca cgcagggcat gaccgtcacc tgcgccaccg
     3601 acggcaatca cgggcgggcg gtcgcctggg gcgcgcagcg gttcggcgcg cgctgcgtca
     3661 tcttcgtgca cgccaccgtc agccagaacc gcgtcgatgc catcgcccgc tatggcgcgg
     3721 aggtccgccg cgtgccgggc acctatgacg atgcggtccg cgaagccgcc cgacaggctg
     3781 aggcgaacgg ctggttcgtg gtctccgaca cgtcctggcc cggctacacc gaggtgccgc
     3841 g
//
LOCUS       DASSZH010000212         2970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_703741, whole genome shotgun sequence.
ACCESSION   DASSZH010000212 DASSZH010000000
VERSION     DASSZH010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703741"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="VFN42_04610"
     CDS             <1..828
                     /locus_tag="VFN42_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET8995928.1"
                     /translation="VLLGDRPALTPVESFYQRMLAGDPDEAQDQAELLLKSRPLSSYY
                     DEVALKGLQLAANDAARCVLSPGQIERVREASFDLIDELAGHEDSDPQPDALETEIAA
                     PSRAEQDIPREPAPRDHAGAALLPPAWQAAAPVLCIAGRGPLDEPVAAMLAQLLGKHG
                     LGARVVPHEAVSRSSIGRLEVDGVAMLCISYLHLEGSLSHLRYLIRRLRQRVPGVPVM
                     VGLWPAEAEILKDDRLRAALGAEYYASSLREAVEGCLDAATHDVPAVDAVPQAVATA"
     gene            932..1324
                     /locus_tag="VFN42_04615"
     CDS             932..1324
                     /locus_tag="VFN42_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET8995929.1"
                     /translation="MRAILLGGALCLAMTCAAQAQIARGKYIVERVGMCGDCHTPRDA
                     KGEPIASKALQGAPLGLRPVHPMPFADAAPRIAGLPENWTLAQAATFLETGKRPNGTE
                     PMPPMPPYRLSKDDALAVATYLKSLKPN"
     gene            1377..2234
                     /locus_tag="VFN42_04620"
     CDS             1377..2234
                     /locus_tag="VFN42_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET8995930.1"
                     /translation="MLRLFALVLCCLFVTLDARAAELKFATWNLAWLTERPAGDPALP
                     PDVHPRHAEDFDLLRQYALRLNADVVALQEVDGRAVAQRVFPPDRYSLHLTQDRRVQR
                     VGIAVRRGLRYDVNPDVAATEGDPDLRLRSEADITLHLPNGDLRILAVHLKTGCAKQR
                     LARSRNLSCADLQAQMRPIAAWIAARRQQGEAFLLMGDFNRWMDSRDQVWRELNRAAP
                     LARATEGRSSPCWGREAFIDHIIAGGSARRWMQRETLRVLVYRETAPDFKARLSDHCP
                     VSVRLRVAE"
     gene            complement(2287..>2970)
                     /locus_tag="VFN42_04625"
     CDS             complement(2287..>2970)
                     /locus_tag="VFN42_04625"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8995931.1"
                     /translation="ALALARDGYRILVTARRSAADCAETVRLVGEAGGEADLHLADIA
                     DPVQARGLIAVAVQRFGRLDVLVNNASLRRQTSFAEITPAEWREIMGTTLDGAFYCAQ
                     AAAPHIIAAGGGSIVNLGGISAHAGAVGRIHAVTAKAGMLGMTRALAKELGGHNITVN
                     VVVPGSIETVRGHAAGGDRGRATLPDSLVGRRGKPEEIAAMVRFLCSPEARYITGQTI
                     HVNGGAFLP"
BASE COUNT          454 a         1003 c         1051 g          462 t
ORIGIN      
        1 gtgctgctgg gcgaccggcc cgcactgacg ccggtggaga gcttctatca gcgcatgctg
       61 gcaggcgacc cggacgaggc gcaggatcag gccgagctgc tgctgaaatc ccgcccgctg
      121 tcttcctatt atgacgaagt ggcattgaag gggttgcagc ttgccgccaa cgatgcggcg
      181 cgttgcgtgc tgagtcccgg ccagatcgaa cgcgtcaggg aagccagctt cgacctgatc
      241 gacgagcttg ccgggcatga agacagcgat ccgcagccgg acgcgctgga gacggagatt
      301 gccgcgccgt cgcgtgccga gcaggacata cccagggagc cggcgccgcg ggaccatgcc
      361 ggagccgcgc tgctgccacc ggcctggcaa gccgccgccc cggtgctgtg catcgccggc
      421 cgcggtccgc tggacgaacc cgtcgcggcc atgctggcgc agcttctggg caagcacggc
      481 ctaggcgcgc gggtggtgcc gcacgaggcg gtttcccgca gctcgatcgg ccggctggag
      541 gtggacggcg tggcgatgct gtgcatctcc tatctgcatc tggaaggcag cctttcgcat
      601 ctgcgctatc tcatccggcg gctgcggcag cgggtgccgg gcgtgccggt gatggtcggc
      661 ctgtggccgg cggaagcgga aatcctgaag gacgaccggc tgcgcgccgc gctgggcgcg
      721 gagtactacg ccagctcgct gcgcgaggcg gtggaaggct gtctggacgc cgctacccat
      781 gacgtccctg cagtggacgc agtgccgcaa gccgtcgcga cggcttgatc cccggtaaat
      841 ttcgtagctg gctttggcct tcggatcggc taagcactgg ggtgcgcaac gtccagcgga
      901 atggacggcc ataagccagg ggagatcggt tatgcgggcc attctgttgg gtggagcgct
      961 ttgcctcgca atgacctgcg ccgcccaggc gcagatcgca cgcggcaaat acatcgtcga
     1021 gcgggtcggc atgtgcggtg attgccacac gccgcgggat gccaagggcg agccaatcgc
     1081 cagcaaggcg ctgcagggcg cgccgctcgg cctgcgtccg gtgcatccca tgcccttcgc
     1141 cgacgcggcg ccaaggatcg ccggcttgcc ggagaactgg acgctggcac aggccgccac
     1201 attcctggag acagggaagc ggccgaacgg cacggagccg atgccgccca tgccgcccta
     1261 tcgtctatcg aaggacgatg cgctggcggt cgcgacgtac ctgaagtcgc tgaagcccaa
     1321 ttgatacaat ctgtggttgc atcatcgggc caagcggcgt agcactgcct gccgcgatgc
     1381 tgcggctgtt cgccctggtc ctgtgctgcc tgtttgtcac cctggatgcc cgggcggcgg
     1441 agctgaagtt cgccacctgg aacctggcgt ggctgaccga gcgcccggcc ggggatccgg
     1501 cgctgccgcc cgacgtgcat ccgcggcacg ccgaggattt cgacctgctg cgacagtatg
     1561 cactgcggct gaacgccgat gtggtggcgt tgcaggaggt ggacggtcgt gcggttgcac
     1621 agcgcgtctt cccgccggac cgctattcgc tgcacctgac gcaggaccgg cgcgtgcagc
     1681 gcgtcggcat cgcggtgcgg cgcgggctgc gctacgacgt gaacccggat gtcgccgcca
     1741 ccgaaggcga tcccgatctg cggctgcgca gcgaggcgga catcacgctg cacctgccaa
     1801 acggcgattt gcgcatcctg gcggttcatc tgaaaaccgg ctgtgcgaaa cagcgcctgg
     1861 cgcgcagcag gaacctgtcc tgcgccgatt tgcaggcaca gatgcggccg atcgccgcct
     1921 ggatcgccgc gcgccgccag caaggcgaag ccttcctgct gatgggcgac ttcaaccgtt
     1981 ggatggacag ccgcgatcag gtctggcggg aactgaaccg ggccgcgccg ctggcccggg
     2041 ccacagaagg ccgttccagc ccgtgctggg gtcgcgaggc gttcatcgac cacattatcg
     2101 ccgggggctc cgcgcgccgc tggatgcagc gggagacgct gcgcgtgctg gtgtaccggg
     2161 aaaccgcgcc ggacttcaaa gcgcggctgt cggaccattg ccccgtctcg gtgcgcttgc
     2221 gcgtggcgga atagctgccg gaacggcggg cggcgaagcc ggaacgaacg gctccggcag
     2281 cgtcaatcag ggcaggaacg cgccgccgtt cacatggatg gtctggccgg tgatgtagcg
     2341 cgcctccggc gagcacagga aacgcaccat ggccgcgatc tcctccggct tgccgcgccg
     2401 tcccaccagg ctgtccggca gcgttgcgcg cccgcgatca ccgcccgccg cgtgcccgcg
     2461 taccgtctcg atcgagcccg gcacgaccac gtttaccgtg atgttgtggc cgccgagttc
     2521 cttcgccaat gcgcgggtca tgcccagcat gcccgccttg gcggtgaccg cgtggatccg
     2581 cccgaccgct ccggcatgcg cggagatgcc gcccaggttg acaatgctgc cgccaccggc
     2641 ggcgatgatg tgcggtgccg ccgcctgcgc gcagtagaag gcgccgtcca atgtggtgcc
     2701 catgatctcg cgccactcgg ccggcgtgat ctcggcgaag ctggtctggc gccgcagcga
     2761 cgcgttgttc accagcacat cgagccggcc gaaccgctgc accgccaccg cgatcaatcc
     2821 gcgcgcctgc accgggtcgg cgatgtccgc caggtgcagg tcggcctcgc cgcccgcttc
     2881 gcccaccagg cggacggtct cggcgcaatc ggcagcggag cggcgagcgg tgaccaggat
     2941 acggtagccg tcgcgcgcca gcgccagcgc
//
LOCUS       DASSZH010000213         5730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_431076, whole genome shotgun sequence.
ACCESSION   DASSZH010000213 DASSZH010000000
VERSION     DASSZH010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5730
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431076"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(349..1101)
                     /locus_tag="VFN42_04630"
     CDS             complement(349..1101)
                     /locus_tag="VFN42_04630"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8995932.1"
                     /translation="MRNVLVTGGSRGLGLGIAHILARAGFRVIAVARRESDQFAAVRE
                     RLHQTPAVAGALHFHAWDLADIEGLPALAKSLHREFGAFYGLVNNAGLGTPGILANMP
                     ESAIEQLVRLNVLSPLTLTKHLVRSMMAGQGTTGGGRIVNISSVVSMTGYNGLSVYSA
                     TKASLIGFTRSLARELGTLGITVNAVAPGFVDTEMTHDLTPAQRERIARRSALQRLPD
                     TADIAHAVEFLFSDKARNITGTVMTVDAGNTA"
     gene            complement(1094..2506)
                     /locus_tag="VFN42_04635"
     CDS             complement(1094..2506)
                     /locus_tag="VFN42_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HET8995933.1"
                     /translation="MKTSQPERRSLRELTASAGLSPHRFLADAAGRVALDGARRDDAP
                     AVPFETLRGRSVLISSDRQLPAVLALLHLDGVAARLVLCPPDLAAAHLPAVIATARID
                     AIVSDVTGPATTAPAGMPVFACPTEAMQPMADGAYTVAAESGPTEWLLFTSGTTGQPK
                     LVVHTLSSLSGPLDDGLAVAPGAVWSTFYDIRRYGGLQILLRALLGGGSMVLSQAGEP
                     IGAFLARVGEAGVTHISGTPSHWRRGLMSPAVKCLSPRYVRLSGEVADQAVLNSLAAA
                     FPAAGIAHAFASTEAGVAFDVRDGLAGFPAAMVGAPGAKAELRIQDGSLRIRSPRIAQ
                     GYLGAGSGLAAADGFVDTGDMVELRGDRYHFIGRREGVINVGGQKVHPEEVEAVINRH
                     PRVQMARVRGRPNPITGALVVADIVVRPPVAPELFPHLREEILTACREALPAHKVPAM
                     LREVAKLDIAESGKLLRRDA"
     gene            complement(2487..2867)
                     /locus_tag="VFN42_04640"
     CDS             complement(2487..2867)
                     /locus_tag="VFN42_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995934.1"
                     /translation="MSVKKGAGGADLGRIADLRSAGRDRSDDAIFWAFIEVGMATLDQ
                     TMSVRNAIIAQIRGIAAQQRKHLAPLSDDLPLLESGLDSLCIAILVASLDDQLDLDPF
                     ADNENTPFPVTLGEFIGLYENIAA"
     gene            3119..4435
                     /locus_tag="VFN42_04645"
     CDS             3119..4435
                     /locus_tag="VFN42_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HET8995935.1"
                     /translation="MRTETIALHAGYQSDSPIRSVAVPVYQTVAYEFDSAEHAAALFN
                     LEADGYRYSRISNPTVAVLERRIAALEGGVGAVCLGSGQAAIHSAMLSLADVGRNIVS
                     VPQLYGTTHTLFSHVLPRLGFEVRIADGDDAADIERLIDAGTVAVYCESVGNPAGNVC
                     DIEAFARVAHAHGVPLMVDNTVASPALLRPIEFGADIVVHSLTKFLGGHGTTLGGAIV
                     DSGRFPWSGHAARFPMLNEPDASYHGLVYAQHFGPAAYIERCRNFCLRTTGAALPALS
                     AFLLLQGVETIALRMERHVQNARHVAMALRDDPRVAWVNYAGFPDNPHYPLVQKYLRG
                     QACSLLTFGVRGGAAAGEAFYDALRLVKRLVNLGDAKTLACHPASTTHRQMTPAQQRR
                     AGVTPEMIRLSVGLEHIEDILADLDQALAAAEACRNEAAGHLVAQA"
     gene            4444..>5730
                     /locus_tag="VFN42_04650"
     CDS             4444..>5730
                     /locus_tag="VFN42_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HET8995936.1"
                     /translation="MPGDVPHSGSALFPPRDQRRAWNNHLTQALDAAEQRIAQGPVTP
                     AFDRAAFHAELAGFDFQQPVPFADLLGWTMVQMEHGLVHITHPRYLGLFNPAPSFPAQ
                     CADRIAASFNPQLATATTSPAAVAIEAHVIRAVARRAGLPADTAGHFTTGGSEANDTA
                     LLCALTRAAPDFAAEGSRAFAGRPVFYTSRDSHLAWIKIAHRSGIGRAAVRLVPTDGA
                     GRMSPDALAAAIAADRREGHIPVMVVATAGTTNAGMVDPLGPCAGIAREAGAWYHVDA
                     AWGGALVASDRLRGLLAGIEQADSVTIDAHKWFATTMGCGMFLTAHPRMLSAAFDVQT
                     SYMPSHTQAVDPYVTTSQWSRRFLGLRLFLSLGTAGWAGHAAHVEQAVARARDLAEGM
                     RRHGWSVLNDPALAVLCLAPPSGARPGRDIVAEVLGS"
BASE COUNT          891 a         2009 c         1928 g          902 t
ORIGIN      
        1 cagcggggtc acacggccag agcaggccgc tatgtcgttt gggcgccgga cgcgaaaccc
       61 atctgagcca gcggcgatgc gccgcgccaa caggccggac acacgaccgc aatcacaccc
      121 gtcgccaaat ccactcaacc ctcttgcgcc aaaggggcct gtccacacag ggaccccgga
      181 cccggtgtgg ttccattatc gcgccgccgg ccgcttcgtg ccctgtgatg cggcgctatt
      241 ggaggtgatc ggccgcggtc tgcgcctgtg atgggcctca tggatcacgt cgcctactga
      301 ggtgcgtcaa tccggcagga cgacttcaca aaatggccaa aaccacgctc aggccgtgtt
      361 gccagcatcg acggtcatca ccgtcccggt gatattgcgc gccttgtcgc tgaacaggaa
      421 ctcgaccgca tgggcgatgt cggcggtgtc cggtaggcgt tgcagggcgc tgcgccgcgc
      481 aatccgctcg cgttgtgctg gtgtcaggtc atgtgtcatc tcggtatcga cgaaaccggg
      541 ggccacggcg ttgacggtga tgccaagcgt gcccagttcg cgcgccagcg accgggtgaa
      601 gccgatcagc gacgccttgg tggcgctgta gaccgacagg ccattgtagc cggtcatgct
      661 caccacggac gagatgttga cgatccgccc gcctcccgtc gtcccctggc cagccatcat
      721 cgagcgcacc aggtgcttgg tcagcgtgag cggcgacagc acgttcagac gcaccagctg
      781 ctcgatcgcg ctctcgggca tgttggcgag gatgccgggg gtgcccaggc cggcattgtt
      841 caccagcccg tagaaggcgc cgaactcgcg gtggagggac ttcgccaggg cgggaaggcc
      901 ctcgatgtcg gcaaggtccc aggcgtggaa atgcagggcg cccgcgaccg ccggcgtctg
      961 gtgcagccgc tcgcggaccg ccgcgaactg gtcgctttcc cgccgcgcca cggcgatcac
     1021 gcggaaccct gcgcgcgcca ggatgtgcgc gatgcccagc cccagcccgc ggctgccacc
     1081 cgtgaccagg acgttacgca tcgcggcgca acagcttgcc ggactcggcg atgtcgagct
     1141 tcgccacctc gcggagcatg gccggcacct tgtgcgccgg cagcgcctcc cggcaggcgg
     1201 tcaggatttc ctcgcgcagg tgcgggaaca attccggcgc gactgggggg cgcacgacga
     1261 tatcggccac aaccagcgcg ccggtgatcg ggttggggcg tccgcgcacc cgcgccatct
     1321 gcaccctcgg gtgccggttg atcaccgcct ccacctcctc gggatgcacc ttctggccgc
     1381 cgacattgat cacaccctcg cggcggccga tgaagtggta gcggtcgccg cgcagttcca
     1441 ccatgtcgcc ggtgtcgacg aagccgtccg cggcggcgag cccgctgcca gcgccgagat
     1501 agccctgcgc gatacgcggc gagcggatgc gtagcgaacc gtcctggatg cgcagctccg
     1561 ccttggcccc cggcgcaccg accatcgcgg cggggaagcc agccaggcca tcgcgcacgt
     1621 cgaaggcgac gcccgcctcg gtggaggcga aggcgtgggc gatcccggcg gcgggaaacg
     1681 ccgcggcgag gctgttcagg accgcctggt ccgccacctc ccccgacagg cggacatagc
     1741 gcggcgagag gcacttcacc gccggactca tcagcccccg gcgccagtgg gatggcgtcc
     1801 ccgatatgtg cgtgaccccg gcttccccga cgcgcgccag gaacgccccg atcggctcgc
     1861 cggcctgcga cagcaccatg gagccgccgc cgagcagggc acgcagcagg atttgcaggc
     1921 cgccatagcg gcggatgtcg tagaacgtgc tccagaccgc ccccggcgcc accgcaagcc
     1981 cgtcatcgag cggcccgctc aggctcgaca gggtatgcac gaccagcttc ggctgcccgg
     2041 tcgtccccga ggtgaacagc agccattccg tcggcccact ttccgccgca accgtgtacg
     2101 caccatccgc cattggctgc atggcctcgg tcgggcaggc gaagacgggc atcccggcgg
     2161 gggcggtcgt cgccggcccg gtgacgtcag agacgatcgc gtcgatccgg gccgtggcga
     2221 tgaccgccgg cagatgcgct gccgccagat ccggcgggca gagcacgagg cgggccgcca
     2281 cgccgtcaag gtgcagcagg gcgagcaccg ccggcaactg ccggtcggag gagatcagca
     2341 ccgaccgacc gcgcaacgtc tcgaacggca ctgccggcgc atcgtcgcgg cgagcgccgt
     2401 ccagcgccac acggcccgcc gcatcggcca ggaaacggtg cggcgacaga ccggcggacg
     2461 cggtcagttc gcggagcgac cgccgctcag gctgcgatgt tttcataaag tccaatgaat
     2521 tcgccaagag tgaccgggaa gggggtgttt tcgttgtccg cgaacggatc aaggtcgagc
     2581 tggtcgtcaa gcgaggcgac caggattgcg atgcacaggg aatcaaggcc ggattcgagc
     2641 agcggcaggt catcggacag cggcgccagg tgctttctct gctgcgccgc gatgcccctg
     2701 atctgcgcga tgatcgcgtt cctgaccgac atcgtctgat ccagcgttgc catgcctacc
     2761 tcgatgaaag cccagaatat cgcgtcatct gagcgatcgc ggccggcgct ccgcaaatca
     2821 gcaattctgc ctaggtcggc cccgcctgcc cctttcttca cgctcaatcc aattgcttac
     2881 ggcggcgact ggccgaatga ccgacaaggg atgcccgcat gccttcgcgc cgggaagcag
     2941 gcgtgccgcg cgccgctggc agcggaaaga cgccgctggc ctcatgcttt ggtatgacgg
     3001 ttgccggcgc ctatcccaaa atcctggcgt tacatccctc cgccatcgcg gcagcaatgc
     3061 ggtcccgatg atgccgtgtt gatctgcacg gcaccgcttc tgcacggagg cccttcccat
     3121 gcggaccgag acgatcgcgc tccatgccgg ctatcagagc gacagcccga tcaggtcggt
     3181 cgctgtgcct gtctaccaga ccgtggcgta tgagttcgat agtgccgaac atgccgcggc
     3241 cttgttcaac ctggaggcgg atggttaccg ctacagccgg atcagcaatc ccaccgtggc
     3301 ggtgctggag cggcgcattg ccgcccttga aggcggggtc ggcgcggtgt gcctcggctc
     3361 cggccaggcg gcgatccaca gcgccatgct cagtctggcc gatgtcggcc gcaacatcgt
     3421 gtccgtcccg cagctttacg gcacgacgca taccctgttc tcgcacgtcc tgccacgtct
     3481 cggctttgag gtgcggatcg ccgacggtga tgacgcggcc gacatcgagc ggctgattga
     3541 tgccggtacg gtcgcggtgt actgcgaaag cgtcggcaat cccgccggca atgtctgcga
     3601 catcgaagcg ttcgcccggg tcgcccatgc gcacggggtg ccgctgatgg tcgacaacac
     3661 ggtcgcctcg ccggcgctgc tgcggccaat cgagttcggc gcggacatcg tcgtccattc
     3721 actcaccaaa ttcctcggcg gccacggcac gaccctgggc ggcgcgatcg tcgacagcgg
     3781 ccgcttcccg tggagcggcc atgccgcgcg ctttccgatg ctgaacgagc cggacgcgtc
     3841 ctatcacggg ctggtctacg cgcagcattt cggtccggcc gcctatatcg agcgctgccg
     3901 caatttctgc ctgcgcacca ccggcgccgc gctgcctgca ttgagcgcct tcttgctgct
     3961 gcaaggggtg gagacgatcg cgttgcgcat ggaacggcac gtgcagaacg cgcggcacgt
     4021 cgccatggcg ttgcgtgatg atccaagggt tgcctgggtc aactatgccg gattccccga
     4081 caacccgcat tatccgcttg tgcagaaata cctgcgcggg caggcctgct cgctgctgac
     4141 cttcggcgtg cgtgggggag ctgcggccgg cgaggccttc tacgacgcgc tgcgcctggt
     4201 caagcggctg gtcaatctgg gtgacgccaa gacgctggcc tgccatccgg cgtccaccac
     4261 gcaccgccag atgacgcccg cgcagcagcg ccgcgccggc gtcacgcccg agatgatccg
     4321 cctcagcgtc ggccttgagc atatcgagga cattctcgcc gatctcgacc aggcgctggc
     4381 ggcggcggag gcgtgtcgga acgaagcagc cggccatctc gtggcgcagg cctgatcccg
     4441 gacatgccgg gcgatgtccc gcatagcggg tcggccctgt tcccgccccg cgaccagcgg
     4501 cgcgcgtgga acaaccatct gacccaggcc ctcgacgcgg cggagcaacg catcgcgcag
     4561 gggccggtca cgccggcgtt cgaccgcgct gcgtttcacg cggaactggc cggcttcgac
     4621 ttccagcagc ccgtgccgtt cgcggatttg ctcggctgga ccatggtgca gatggagcat
     4681 ggcctcgtcc acatcactca tccgcgctac ctgggcctgt tcaatccggc cccgagcttt
     4741 cccgcgcaat gcgccgaccg gatcgcggcg agtttcaatc cgcagctcgc caccgccaca
     4801 acctcgccgg ccgcggttgc aatcgaggcc catgtcatcc gcgccgtcgc gcggcgcgcc
     4861 ggcctgccgg cggacacggc cgggcacttc accaccggcg ggtcggaggc gaacgacacc
     4921 gcgctgctct gcgcgttgac ccgcgccgca ccggatttcg ccgccgaggg cagccgcgcc
     4981 ttcgcaggcc ggccggtgtt ctacacctcc cgtgacagcc acctagcctg gatcaagatc
     5041 gcgcaccggt ccggcatcgg ccgggcggcg gttcggctgg tgccgaccga cggtgcagga
     5101 cgcatgagtc cggatgcatt ggccgccgcc atcgcggctg atcgccggga gggacacatc
     5161 cctgtcatgg tcgtggcgac ggcgggcacg accaatgccg gaatggttga tccgctcggg
     5221 ccatgcgccg ggatcgcccg cgaggccggc gcctggtacc atgtcgatgc ggcctggggt
     5281 ggcgcgctgg tcgcctcgga ccggctgcgt ggcctgctgg ccgggatcga acaggccgat
     5341 tccgttacca tcgacgcgca taagtggttc gcgaccacga tgggctgcgg catgttcctg
     5401 acggcgcatc cacggatgct gtcggccgcg ttcgacgtgc agaccagcta tatgccgtcg
     5461 cacacgcagg ccgtcgatcc gtacgtgacc acgtcccagt ggtcacgccg cttcctcggc
     5521 ctgcggctgt tcctgtcgct cgggacggcc ggatgggccg gacatgccgc gcatgtggag
     5581 caggcggtcg ccagggcgcg cgacctggcg gagggaatgc gccggcatgg ctggtcggtc
     5641 ctcaacgacc cggcgctggc cgtgctctgc ctggcgccgc cgtcgggcgc acggccgggg
     5701 cgcgatatcg tcgccgaggt gctcggctcc
//
LOCUS       DASSZH010000214         2168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_38836, whole genome shotgun sequence.
ACCESSION   DASSZH010000214 DASSZH010000000
VERSION     DASSZH010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38836"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /gene="ispF"
                     /locus_tag="VFN42_04655"
     CDS             <1..748
                     /gene="ispF"
                     /locus_tag="VFN42_04655"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HET8995937.1"
                     /translation="ADTLKRVREGIIAETVPRDGLYRAQTPQAVRFPVLLAAHRAPVA
                     GATDDASLLEAAGETVAIVPGAEENIKLTYPEDLARLERMMAPTLIPRVGTGFDVHVL
                     ETGRKLMLCGVHVPHDQGLAGHSDADVGIHALCDAIYGALAEGDIGRHFPPSQAAWKD
                     ADSARFLRHAAERIAARGGVLANADVTLICEQPKIAPHAAAMMQRLAALLGVDVARVS
                     VKATTTERLGFTGRGEGIAAQAIATILLPM"
     gene            827..1141
                     /locus_tag="VFN42_04660"
     CDS             827..1141
                     /locus_tag="VFN42_04660"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995938.1"
                     /translation="MTYRFAPVVLVLAGLLAGCATETTTSTGNPYPPVPPPIAESRPL
                     PPVSAQALMWQPGHWDWNGSGYVWQKGQYVPGAGHGPLFQPGYWTQTPSGWSWQPAHW
                     TS"
     gene            1329..>2168
                     /locus_tag="VFN42_04665"
     CDS             1329..>2168
                     /locus_tag="VFN42_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET8995939.1"
                     /translation="MSSSLANDPTTAIRAIAERIEPQLIEIRRDIHAHPELGFEEVRT
                     AGVVTRELTRLGIPHQTGIGKTGVVGLIEGGRPGPVLAIRADMDALPIQEETGLPFAS
                     QNAGLMHACGHDIHTTTLIGVAEVLKEIAPQLAGTVKLVFQPAEEGVGGMRAMIADGL
                     MLSPKIDMALGFHNHPDMAVGHFGFVHGACLAASDRFDIVVRGRSGHAAYPHGAIDPI
                     MAAATLVTQLQTVVSREVPPTMAAVVTVGAIHGGTARNIIPDSCTLLGTVRTLHPMAR
                     DIAE"
BASE COUNT          337 a          751 c          755 g          325 t
ORIGIN      
        1 cgccgacacg ctgaaacggg tccgcgaggg tatcatcgcc gaaaccgtgc ctcgcgacgg
       61 gctgtaccgg gcgcagaccc cgcaggccgt ccgctttccc gtgctgctgg cagcgcatcg
      121 cgcgccggtg gcaggcgcca ccgatgatgc gtcgctgctg gaggccgccg gcgagacggt
      181 ggcgatcgtt cccggcgccg aggaaaacat caagctgacc taccccgaag accttgctcg
      241 gctggagcgc atgatggccc ccacgctgat cccgcgcgtc ggtaccggct tcgacgtgca
      301 tgtgctggag accggccgca agctgatgct gtgcggcgtg cacgtgccgc atgaccaggg
      361 cctggccggg cactccgacg ccgatgtcgg catccacgcg ctgtgcgacg cgatctacgg
      421 tgcgctggcc gagggcgata tcggccggca tttcccaccc agccaggccg cgtggaagga
      481 tgccgacagc gcccgcttcc tgcgccacgc ggcggagcgg atcgccgcgc gcggcggcgt
      541 gctggccaat gccgacgtca cgctgatctg cgagcagcca aaaatcgccc cgcacgcggc
      601 ggccatgatg cagcggctgg cggcgctgct gggggtggac gtggcgcgcg tatcggtgaa
      661 agccaccacg accgagcggc ttggcttcac cggacgcggc gagggcatcg ccgctcaggc
      721 aatcgcgacc atcctgttgc cgatgtaacg gatttgttgt ggaaaccggc cggccgcgtg
      781 gccatttccg gtatcaagtt tcgcttgcaa gctgaccgga gggccgatga cgtaccgctt
      841 cgcacctgtt gtgctggtcc tggccggcct gctggctggc tgtgccacgg aaaccacgac
      901 aagcacgggc aatccctacc cgccggtgcc tccgccgatc gcggagagcc gtccgctgcc
      961 gccggtcagc gcgcaggcgc tgatgtggca gcccgggcac tgggactgga acggctcggg
     1021 ttatgtctgg cagaagggcc agtatgtgcc gggcgcgggg catggtccgc tgttccagcc
     1081 gggttactgg acgcagaccc cgtcaggctg gagctggcag ccggcgcact ggacgtcgta
     1141 gggcgaagct gggacgtctt cgggattgat gaaccgtttg gccgagcgcg gcagtcgctc
     1201 ggagatcagg ccgggcggcg ccgccggctg aacgggtcac ggccggcgct ggggatgcga
     1261 agctccgggt tgcggcgggg cggcgcaacc cgcacaatgc cggcgtcata cgcaacaggc
     1321 cgcttcccat gtcctccagc ctagccaacg accccaccac cgcgatccgt gccatcgcgg
     1381 agcgcatcga gccgcagctg atcgagatcc gccgcgacat ccacgcgcat cccgaactcg
     1441 gcttcgagga ggtccgcacc gccggcgtcg tgacgcgcga actcacccgt ctgggcatcc
     1501 cgcatcagac cggcatcggc aagaccggtg ttgtggggct gatcgagggt gggcgtcccg
     1561 gcccggtgct ggcgatccgc gccgacatgg acgcgctgcc gatccaggaa gagaccggcc
     1621 tgcccttcgc cagccagaac gccggcctga tgcacgcctg cggccacgac atccatacca
     1681 ccacgctgat cggcgtcgcc gaggtgctga aagagatcgc gccgcaactc gccggcacgg
     1741 tgaagctggt gttccagccg gccgaggaag gtgtcggcgg catgcgcgcc atgatcgcgg
     1801 acggcctgat gctgtccccc aagatcgaca tggcgctggg cttccacaat cacccggaca
     1861 tggcggtggg gcatttcggc ttcgtgcacg gcgcctgcct cgcggcttcc gaccggttcg
     1921 acatcgtcgt gcgcgggcgg tccggtcatg ccgcctatcc gcacggcgcg atcgacccga
     1981 tcatggcggc ggcgaccctg gtgacgcagt tgcagaccgt ggtatcgcgc gaagtgcccc
     2041 ccaccatggc ggcggtggtg acggtgggcg ccatccatgg cggcaccgca cgcaacatca
     2101 tccccgattc ctgcaccctg ctcggcaccg tgcgcacgct gcacccgatg gcgcgcgaca
     2161 tcgccgaa
//
LOCUS       DASSZH010000215         2074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_584326, whole genome shotgun sequence.
ACCESSION   DASSZH010000215 DASSZH010000000
VERSION     DASSZH010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584326"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="VFN42_04670"
     CDS             <1..867
                     /locus_tag="VFN42_04670"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HET8995940.1"
                     /translation="VLASMAPGGVAAAVVSAIADLPMIRRNPETKRLEKVRDPEPGEC
                     LACVEDYLTGFEATGMRVAREPHEIRAGDPALVLAIEGCDFLEGDLDRLDAMAARGVR
                     SIQLTHYLVNETGDIQTAAPVHNGLTRFGAEAVRRLNRLGIMVDVAHCSEDTVKGVVA
                     AASRPILCSHANLKEPGHKDGDHPRYLSPDYARMVVETGGVIGAWIAVLWEERLPGMI
                     RQLFRLIDAVGIDHVGIGSDLPAGAAKEALPDFSRHPQIAAALRDRGMTEDEVDKVCA
                     GNWLRVFRAARA"
     gene            867..1559
                     /gene="rpiA"
                     /locus_tag="VFN42_04675"
     CDS             867..1559
                     /gene="rpiA"
                     /locus_tag="VFN42_04675"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HET8995941.1"
                     /translation="MDRDEQKRAAAEAAAALVQPGMVVGLGTGSTAAFAIDALIRRVR
                     QGLDIVGIPSSERSAAQARAGGIRLVDFAEHRRIDLTIDGADEILPGPLHLVKGHGGA
                     LLREKIVAAASARLVIMADDSKLVDRLGRKMSVPIEVVTFGWQTTADRLSALGGAPTL
                     RRGADGAPFRTDGGNVILDCAFGPIADPADLERRLGQVVGLVETGLFIGMADLALVAT
                     AGGVQRLERSGN"
     gene            complement(1604..>2074)
                     /locus_tag="VFN42_04680"
     CDS             complement(1604..>2074)
                     /locus_tag="VFN42_04680"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HET8995942.1"
                     /translation="GSNPERDHDMTMALNTSFTNMDVPAERRTTRVAGRFSLISAAHA
                     ATIDRAKRGAIREAHVRRVVEREAAPVSGWIVQVGSFSNPHAALVAAEAARRHARRGD
                     PHVERIRLRRRTWFRAQVTGFTEASAQEACAVIARHQRARCLILRPEHPVVASR"
BASE COUNT          299 a          696 c          770 g          309 t
ORIGIN      
        1 gtgctggcct ccatggcgcc gggcggcgtg gccgcggcgg tggtctccgc catcgccgac
       61 ctgccgatga tccgccgcaa cccggaaacg aagcggctgg agaaggtgcg tgacccggag
      121 cccggcgagt gcctggcctg cgtggaggac tacctcaccg gcttcgaagc caccggcatg
      181 cgggtggccc gggaaccgca cgagattcgc gccggcgacc cggccctggt gctggcgatc
      241 gagggttgcg acttcctgga aggcgatctc gaccggctgg atgccatggc ggcgcgcggc
      301 gtccgcagca tccagttgac gcattacctg gtgaacgaaa ccggcgacat tcagaccgcc
      361 gcgccggtgc acaacggcct gacccggttc ggcgccgagg cggtgcggcg gctgaaccgg
      421 ctgggcatca tggtggacgt ggcgcactgc tcggaggaca cggtgaaggg cgtggtggct
      481 gcggcctccc gcccgatcct gtgcagccat gccaacctga aggaacccgg ccacaaggac
      541 ggcgaccacc cgcgctatct cagcccggac tatgcccgta tggtggtgga aaccggcggg
      601 gtgatcggcg cctggatcgc cgtgctgtgg gaggagcggc tgccgggcat gatccgccag
      661 ttgttccgcc tgatcgacgc ggtcggcatc gaccatgtcg gcatcggcag cgacctgccg
      721 gccggcgccg ccaaggaggc gctgccggat ttctcccgcc acccacagat cgccgccgcg
      781 ttgcgggacc gcggcatgac ggaggacgaa gtggacaaag tctgcgccgg caactggctg
      841 cgcgtgttcc gggcggcccg ggcgtgatcg atcgggacga acagaagcgt gccgccgccg
      901 aggccgccgc cgcgctggtg cagcccggca tggtggtcgg actgggcacc ggttccaccg
      961 cggccttcgc gatcgacgcg ctgatccgcc gggtgcgcca gggcctggac atcgtcggca
     1021 tccccagttc cgaacgcagc gccgcgcagg cgcgcgcggg gggcatccgc ctggtcgatt
     1081 tcgcggagca tcggcggatc gacctgacca tcgatggcgc ggacgaaatc ctgcccggcc
     1141 cgcttcacct ggtgaagggc cacggcggag cgttgctgcg tgagaagatc gtcgccgccg
     1201 ccagcgcccg gctggtgatc atggcggatg actccaagct ggtggaccgg ctcggccgca
     1261 agatgagcgt gccgatcgaa gtggtaacct tcggctggca gaccacggcc gaccgcctta
     1321 gcgccctggg aggcgctccg accctgcggc gcggcgccga tggcgcgccg ttccggaccg
     1381 acggcggcaa cgtgatcctg gactgcgcct tcggtccgat cgccgatccg gccgacctgg
     1441 aacggcggct gggtcaggtg gtgggactgg tggaaaccgg gttgttcatt ggcatggccg
     1501 atctggccct ggtcgccacc gccggcgggg tgcagcggct ggagcggtcg ggcaactgaa
     1561 cctctacccc tcccgcaagg ggaagggaga ggttctgttt cccttaccgg ctggccacaa
     1621 cgggatgctc cggccgcagg atcaggcaac gcgcccgctg atgccgtgcg atcaccgcgc
     1681 aggcttcctg cgccgaggcc tcggtgaagc cggtgacctg cgcgcggaac caggtgcggc
     1741 gccgcaggcg gatgcgctcc acatgtgggt cgccgcgccg ggcgtgacgc cgagctgcct
     1801 cggccgctac cagcgcggcg tgtgggttgc tgaagctgcc gacctggacg atccagccgg
     1861 aaactggcgc tgcctcgcgc tccacgaccc gccggacatg cgcttcacgt atcgcgcccc
     1921 gcttcgcgcg gtcgatggtt gcggcatggg ccgccgagat caggctgaac cggccggcga
     1981 cgcgggtggt ccggcgctcg gcgggaacgt ccatgttggt gaagctggta ttgagcgcca
     2041 tggtcatgtc gtggtcgcgc tcggggttgg atcc
//
LOCUS       DASSZH010000216         3515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_22074, whole genome shotgun sequence.
ACCESSION   DASSZH010000216 DASSZH010000000
VERSION     DASSZH010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22074"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="VFN42_04685"
     CDS             complement(<1..1095)
                     /locus_tag="VFN42_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995943.1"
                     /translation="MVKASHDLAAPAWLPQRELGELHKSLASAQDAQITQLVAMVDRL
                     RDRGAIDDLVAPFRARLARLRPARPLRFSRLLFNPVDPLIVPAPRWRLESPCLPRTVL
                     APLAEAAHATMGAEAAQIDAAIAGHSTRDLSVIAQAGALLWPSAAQALLRSGVPAGWV
                     RQTGLSQAMFRHIAANVSAVLDQVLALQTLRAEAEVGAPLRLQVLTTMLRQVNAARPE
                     ALGLLIALVLARLPEAGPLLRKAGTEIGGVAAVAVRAATEQAKCSLLDRLEAEGGTEA
                     LVVGTGLTEVGAAVRRIATLLQSMESGGAGSAQRVEQLRQRLDVSCRTRFTAGLQNEF
                     VRSLEALVGSPDDAAVMRLEEAARGLRELGM"
     gene            complement(1111..2187)
                     /locus_tag="VFN42_04690"
     CDS             complement(1111..2187)
                     /locus_tag="VFN42_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995944.1"
                     /translation="MPVTFLERGVQVPFTTPMLAGTRARPAEAYGTELLVPNLSGGRG
                     VYVLPWAGVQKLCCPTVHDRRLNQKVETLITVTPSTIRHAAREVAVEGLAGREAQVAA
                     VRAAELDRRGRMRTNFLLLVALLDRLEPGSLNPAWMGEPSLELEAQACGTIVARAPQL
                     GLAPDTIIADLEQLAEVLSAIGVHAEGPPARIPLLLEMLSDLHEQTTAWSRANCGEHA
                     DIAAMIAEVAGATITCAAATLRDARRLTEDVVRLVRQWSSNAGAVVRLAARPEWLLDG
                     WEQICLLWRDAEMPGAREAALAEMALLAPVLPKETRLWVRAPIQEHSLFTYRRTVLAN
                     EDWRTGSAGLELIWRNERLRARCA"
     gene            2386..>3515
                     /locus_tag="VFN42_04695"
     CDS             2386..>3515
                     /locus_tag="VFN42_04695"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET8995945.1"
                     /translation="MRTGIRLLAAVLGWGFISGSATADMLSSLLPAGVPGYGIAPGVT
                     VTSRLRTETESPGIRAGGFMLHPELEEAAGYDSAPFGAGSGGSWRVDTHPSLLVGSAW
                     SRDALGAYFAATNRHYLAAAAQDRTDYTVSLGGSLDIGRDRLTLSAAHLLQHQDRTQL
                     GALFTDRPVAFQLNDARLSYAANAGRWTVTPALQIASFQFGSASLQGLPLDQSYRDRR
                     LLEASLRLDYALAPRRVLLLEVRGLGQHYLHAQPDQPERDATGYQLLAGFADDDGGLW
                     GYRVLAGVESRQFQAAAYRTHTAAIGEAALVWNPSGLTTVTATLTRSMEDAAQEGAAG
                     YTYTAATLRIDHEYRRDVLLHASGQAQRADFLPNGGSQTGYAA"
BASE COUNT          544 a         1256 c         1196 g          519 t
ORIGIN      
        1 catgccgagc tcccgcaggc cgcgcgcagc ctcctccaga cgcatgacgg ccgcgtcgtc
       61 cggactgccg accagcgcct caagggagcg gacaaactca ttctgcaggc cggcggtaaa
      121 gcgggtgcgg cagctcacgt ccaagcgttg ccgaagctgc tccacgcgct gggccgagcc
      181 ggcgccgccg gattccatgc tctgcagcag tgtggcaatg cggcgcaccg cggcgccgac
      241 ctccgtcagg ccggtaccga ccaccagagc ctcggtgccg ccttcagcct ccagccggtc
      301 cagcaggctg cacttcgcct gctcggtcgc cgcgcgcacc gcgaccgccg ccaccccacc
      361 gatttcggtc ccagctttgc gcagcaacgg cccggcctcc ggcaggcggg ccaggaccag
      421 cgcgatcagc aagcccagcg cttccggccg agcggcgttc acctggcgga gcattgtggt
      481 caggacctgc agccggaggg gcgcgcccac ctcggcctcg gcacgcaggg tctgcagcgc
      541 caatacctgg tccagcaccg cggatacatt cgccgcgatg tggcgaaaca tcgcctgcga
      601 caggccggtc tgccgaaccc agccggccgg cacgccactg cgcagcagtg cctgtgctgc
      661 gctgggccac aacagcgccc cggcttgtgc gatcaccgac aggtcgcgtg tggagtgtcc
      721 ggcgatcgcc gcatcgatct gcgccgcttc cgcgcccatc gtcgcgtgcg cggcttcggc
      781 cagcggcgcc aggacggtgc gtggcaggca gggggattcg agccgccaac ggggggccgg
      841 cacgatcagc ggatccaccg gattgaacag caaccggctg aaccggagcg gccgcgccgg
      901 ccggaggcgc gccaggcgcg cgcggaaggg cgcgaccaga tcgtcgatcg ctccgcgatc
      961 gcggagccgg tccaccatcg cgacaagctg ggtgatctgg gcgtcctgcg cgctggccag
     1021 gctcttgtgc agctcgccca gttcgcgctg cggcagccag gccggcgcgg cgagatcatg
     1081 cgatgccttg accatcccgg gccccttcct tcatgcgcat cgggcgcgca gccgctcgtt
     1141 acgccagatc agctccagac cggcgctgcc ggtgcgccag tcctcattgg ccaggacggt
     1201 gcgccgatag gtgaacaggc tgtgctcctg aatcggcgcg cgcacccaaa ggcgggtttc
     1261 cttcggcagg accggggcga gtaacgccat ctcggccagg gccgcctcgc gcgcgccggg
     1321 catctccgca tcgcgccaca acaggcagat ctgctcccag ccatccagca gccattccgg
     1381 acgcgccgcc aggcggacca ctgcgcctgc attgctgctc cactgccgca ccaaccgcac
     1441 cacatcctcg gtcagcctgc gggcatcccg cagggtggcg gcggcacagg tgatggtggc
     1501 accggccacc tcggcgatca tcgcggcaat gtcggcatgt tccccgcaat tcgcccgcga
     1561 ccacgcggtg gtttgctcat gcaagtccga cagcatctcc agcagcaaag gtatgcgggc
     1621 cggaggacct tccgcatgca cgccgatggc actgagcacc tcggcgagct gctccaggtc
     1681 ggcaatgatg gtgtccggtg ccaggccaag ctgcggggcg cgggcgacga tggtgccgca
     1741 ggcctgagct tccagttcga gcgagggctc gcccatccag gctgggttca ggctgcccgg
     1801 ttccaggcgg tccagaaggg cgaccagcag caggaaattg gtgcgcatcc tgccgcggcg
     1861 gtccagttcg gcggcccgca ccgcggcgac ctgcgcctca cggccggcca gaccctccac
     1921 ggcgacctcc cgcgctgcgt gccggatggt gctgggcgtg acggtgatca gtgtctccac
     1981 cttctggttc agccgccggt cgtgcacggt ggggcagcag agcttctgca cacctgccca
     2041 gggcagcaca tagacgccac gcccccctga caggttgggc accagcagtt ccgtgccata
     2101 cgcctccgcc ggccgggcgc gggtgccagc caacatcggc gtggtgaagg gcacctgaac
     2161 gccacgctcc aggaacgtga ccggcacata ggccttcggg tgaggtagcc ggaatgccgg
     2221 catttgcggg accgcctcgc caatgctgcc gcagcatgcc gaacgatcct gaacaaacgg
     2281 ttaggacccc gcccggatcg cgaaagcgtc tgggcgagtc ccgctgcggg gcaaaatccg
     2341 ccggcattaa cggggcgcta gtgagtcggt gcgacactgc ccggcatgcg caccggaatt
     2401 cgtcttctcg cggcagtgct gggctggggc ttcatcagcg gcagcgcgac ggcggacatg
     2461 ctttcctctc tgctgccggc cggcgtcccc ggctacggca tcgcgcccgg cgtgaccgtc
     2521 acctcccgcc tgcgtactga aacggagtcg cccggcatcc gtgccggcgg gttcatgctg
     2581 cacccggagc tggaggaagc cgccggctac gacagcgcgc cgttcggtgc gggctcgggc
     2641 ggaagttggc gggtggacac ccatccctcg ctgctggtcg gcagcgcgtg gtcgcgcgat
     2701 gcgctggggg cgtattttgc ggcaacgaac cggcattacc ttgccgcggc ggcgcaggac
     2761 cgtacggatt acacggtgtc gctgggtggt tcgctggaca tcggccgcga ccggctgacg
     2821 ctgtcggcgg cgcatctgct gcagcatcag gatcgtaccc agcttggcgc actgtttacc
     2881 gatcgcccgg tggcgttcca gttgaacgac gcccgcttgt cctatgccgc aaacgcaggc
     2941 cgctggacgg tgaccccggc gctgcaaatc gcgtccttcc agttcggaag cgcaagcttg
     3001 cagggcttgc cgctggacca gagctaccgc gaccgcaggc tgctggaagc cagcctgcgg
     3061 cttgactatg ccctggcgcc gcggcgcgtg ttgctgctgg aggtgcgggg tctggggcag
     3121 cactacctgc acgcccagcc ggaccagccg gaacgcgatg ccaccggtta ccaactcctc
     3181 gccggttttg ccgacgatga cggcgggcta tggggctacc gagtattggc cggcgtggaa
     3241 tcgcggcaat tccaggctgc tgcgtatcgg acgcacaccg cggcgatcgg tgaggccgcc
     3301 ctggtgtgga accccagcgg gctcaccacg gtaaccgcga ccctcacccg cagcatggag
     3361 gatgccgcgc aggaaggcgc cgctggctac acgtacaccg cggcgacgct gcggatcgac
     3421 cacgagtacc gccgcgacgt tctgctgcac gcctccggcc aggcgcaacg cgccgacttc
     3481 ctgccgaacg gaggatcgca gaccggatac gcagc
//
LOCUS       DASSZH010000217         3051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1232491, whole genome shotgun sequence.
ACCESSION   DASSZH010000217 DASSZH010000000
VERSION     DASSZH010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232491"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1216..>3051)
                     /locus_tag="VFN42_04700"
     CDS             complement(1216..>3051)
                     /locus_tag="VFN42_04700"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HET8995946.1"
                     /translation="VTINGFEISGVSVSSGNGAAIRYEGGTLTLNNDFFHNNEDGMLA
                     ASDPSGSITISHSEFAFNGTGDGQTHNLYVNDVGTLTITGSYFHDANVGHEIKSRAEN
                     TTITNTRIFDNSSPASYSIDLPNGGNATIQNNTIEKGANSQNGAFISYGEEGLVNTGT
                     TVLIANNTFVNDRYSAVAVTGTATTPLLFQNNQLYGMNSGQIGSPVSESGTTYLSTRP
                     TLDTSSLQAPCFAEGTRILTARGEVPVQALTESDRIVTGSGALQRLVWLGHRRIDLRR
                     HPRRDLAAPVRICRDAFGPGMPRRDLRVSPDHCLFVDGGLIPAKLLINGMTIVLERET
                     PSVTYYHVELERHAILLAENLPVESYLDTGNRALFANAGLALVLHPEFQVNTGLKHWA
                     EHACAPLTVAPDAVEPVWRRLATRARALGCSPPNRATTEDPLLRVQVQGRTLRSLACR
                     NGRAVFALPPGTQAVRLTSRSTVPSHTQPWVDDWRRLGVAVRRLELRCGGDRMPIALD
                     GPGLAAGWHAVERDDASMWRWTNGDAVVDLLDCSGPMLLEVDVAITLRYPVADAQGTV
                     GNGTSQYGSERAANSDNPVRASASNAARHQSGAGGKDLWLNVASR"
BASE COUNT          474 a         1048 c          993 g          536 t
ORIGIN      
        1 ggcgaagttc gaggggcaga agcggctcgc cgcccggctg ttccaccatc agcaggtggc
       61 gctgggtggg gaacgaaccg ccggcaacct ggacacgttc cgcctgttcg ccgaacgcaa
      121 gcagcaactg cttgggcgcg ccggcgcaat cggcatgctg gtgccctccg ccttccacgc
      181 caacgagggc acgaccgcgt tgcggatgct ctacctggag cgcacaagcc tggaaacctg
      241 cctgtgtttc gagaaccgcc gcaagctgtt cgacatcgac agccgctaca aattcgccct
      301 ggtggtcgcc cgcgcacccg gaccgacccg cacggtgcga tgtgccttct acctggacgc
      361 cttcgagcag accgaagacc cggcgcgcat cctgagctac gaccaggcat tcatcgacgc
      421 cacgggaggc gcgcatcgct gcctgccgga actgcgcagc cgcgaccacc tggcactggc
      481 acgccttctg ttcctgggcc atggcacgct gggtgactgg tgcagcgcgc gcggcatcac
      541 cttcggccgc gatctgcaca tgaccgacga cgcgccgcat ttcgtcccgg caagccaggc
      601 gttgggcacg aagcctggcg cctgcctgct gctgcacgag ggcaagacct tccaccagtt
      661 caccgactgc tgggacaacg tcccgcgcta tgccgttccc gccgccgcac tgcaccgcaa
      721 gccggcaacc caggcggcgt gccggcattt ccgcctggtg tttcgtgaca tcgcgcgttc
      781 ctcggacgaa cacacactga tcgcggcgat cgcaccgccc ggggtggtgt tcggccatac
      841 tgcaaacacc gagcgcacac cgcatcgccg tcccgtcagc caggccttgc tgctgtgcgc
      901 cttgctcaac agcttcgtgg tggattgggc ggtgcggcag aaagccgcga cccatctgag
      961 cctgttcatc atccaggctc tgccgctgcc cggtttgccg gaagcggcac agcgcctgct
     1021 ggcgcacgcc gcgctgcggc tgagctgcaa tcaccccggc taccagccgc tatggcgcga
     1081 tcagcttggc gatgtctggc gcgaaccgca tccgggctgg ccggtactgt ccgagccgga
     1141 tgcgcgctgg cgcgttcgcg ccgccaacga tgcggcgatc gcgcactgct tcggcctcga
     1201 tcgcgacgcc taccgtcacc tgctggcgac gttcagccac aaatcctttc cgccggcgcc
     1261 ggactggtgc cttgcggcgt tcgacgcgct ggcgcggacc gggttgtcgg aattcgccgc
     1321 ccgctccgat ccatactggg acgtcccgtt gccgacagta ccctgcgcat ccgccaccgg
     1381 atagcgcagc gtgatcgcca catcgacttc cagcagcatc ggccccgaac aatccagcag
     1441 atccaccaca gcgtcgccgt tggtccatcg ccacatgctg gcgtcgtcgc gttcgaccgc
     1501 atgccagcct gcggcaagtc ccggaccatc cagtgcaatg ggcatgcgat cgccgccgca
     1561 gcgcagttcc agccggcgca ccgcaacgcc gagccgccgc cagtcatcga cccagggctg
     1621 ggtgtgcgac ggcacggtgg agcgggatgt cagccgcacc gcttgcgtgc cgggcggcag
     1681 cgcaaacacg gcgcgcccgt tgcggcacgc cagggagcgc agcgtgcggc cctgcacctg
     1741 cacgcgcagc agcgggtctt ccgtggtggc acgattcggc ggcgagcaac ccaacgcccg
     1801 cgcgcgtgtt gcaagccggc gccacaccgg ttcaaccgcg tccggcgcca cggtcagcgg
     1861 tgcgcaggca tgctctgccc agtgcttcag gccggtattc acctggaact cgggatgcag
     1921 caccagcgcc aggccggcat tggcgaacag cgcgcggttg ccggtgtcca gatagctttc
     1981 caccggcagg ttttccgcca gcaggatggc gtgccgttcc agttccacat gatagtacgt
     2041 caccgacggt gtttcgcgct ccagcacgat cgtcatgccg ttgatcagca gtttcgccgg
     2101 gatcagcccg ccgtcgacga acaggcaatg gtccggcgag acgcgcaggt cccgccgcgg
     2161 catgccgggt ccgaacgcat cgcggcagat ccgcaccggc gccgccaggt cgcgcctggg
     2221 atggcgccgc agatcgatgc gccggtgccc aagccacacc aggcgctgca gcgcgccgga
     2281 gccggtgacg atgcggtcgc tttcggtcag ggcctgcacc ggaacctcgc cgcgcgcggt
     2341 caggatgcgc gtgccctcgg cgaaacaggg ggcctgcaat gaactggtat ccaacgtcgg
     2401 gcgcgtgctc aggtaggtgg tgccgctctc gctcaccgga ctgccgatct ggccgctgtt
     2461 catcccgtac agctggttgt tctggaacag cagcggggtg gtggctgtgc cagttaccgc
     2521 gaccgcggaa tagcggtcgt tgacgaaggt gttgttggcg atcagcacgg tggtgccggt
     2581 gttcaccagc ccctcctcgc cgtagctgat gaaggcgccg ttctggctgt tcgcgccctt
     2641 ctcgattgta ttgttctgga tcgtcgcgtt gccgccgttc ggcaggtcga tgctgtagct
     2701 ggcgggactg ctgttatcga agatccgcgt attggtgatc gtggtgttct cggcacggct
     2761 cttgatctcg tggccgacat tcgcatcgtg gaaatagctg ccggtgatgg tcagggtgcc
     2821 gacgtcgttg acgtacaggt tgtgcgtctg cccatcgccc gtgccgttga acgcgaactc
     2881 ggaatggctg atggtgatgc tgccggacgg atcgctggcg gccagcattc cgtcttcgtt
     2941 gttgtggaaa aagtcgttgt tcagcgtcag tgtgccgcct tcatagcgga tcgccgcacc
     3001 gttgccgctg gagacgctga cgccgctgat ctcgaagcca ttgatggtga c
//
LOCUS       DASSZH010000218         3653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1436880, whole genome shotgun sequence.
ACCESSION   DASSZH010000218 DASSZH010000000
VERSION     DASSZH010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436880"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VFN42_04705"
     CDS             complement(<1..189)
                     /locus_tag="VFN42_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HET8995947.1"
                     /translation="MSRISRIRPVHPWPLRLMHWLNAVAIVTMIGSGWQIYNAAPLFA
                     FSFPRRFTIGEWLGAAIAW"
     gene            389..640
                     /locus_tag="VFN42_04710"
     CDS             389..640
                     /locus_tag="VFN42_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995948.1"
                     /translation="MTKYLLVAAAAVALALAPAAFAQTADMSGNMGHDPAMAHGCKAG
                     KDAMGHPCKPDAMRHDTMGHDAMAHEMGHDAMGHATSAN"
     gene            735..1403
                     /locus_tag="VFN42_04715"
     CDS             735..1403
                     /locus_tag="VFN42_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HET8995949.1"
                     /translation="MPSLQEQNLGEQLRLRRLGQSTEVRVVYEAMVGELARSGFIEHV
                     LPVGARFPDVALPTAEGELARLADWWRRGPLVVTFFRGEWCPYCRLVLAALEEVLPEI
                     EALGATLVAVTPETGGRALTAKRRHNNRYEMLSDVDCGLGLDCGVVFQAPAAYRDMLL
                     RFGNDLAERHGNDAWFLPVPATFVLDRDGIVRWRFAEVDFTKRAEPADILAALRDLAA
                     SSAG"
     gene            1416..2324
                     /locus_tag="VFN42_04720"
     CDS             1416..2324
                     /locus_tag="VFN42_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET8995950.1"
                     /translation="MELTQVRYFVALCRTLNFTRAAETCNVTQPALTRAIQRLEDELG
                     GPLLYRERTLTQLTELGRAMRPHLEAMLEAAEAARQAARACVGLEAASVRIGLGPAIA
                     AAHVAGAIRGLVRLYPELSIHFEEAGTAALAEAMLSDMLDCAVLPDDPGLPERLNRWK
                     IAEEGCIVVLPGDHALAARTGLTADDLAGETVLLGERCGGFAHRLRAACGDRFAQRPC
                     GGSWVQMLDLVRAGLGLALLPDGLAVGGGLAVRPLVEPCLRRSLMLTLVAGRPHSAAV
                     ANLVKLCRARGPGAAQPDGARTQRPA"
     gene            complement(2438..3079)
                     /locus_tag="VFN42_04725"
     CDS             complement(2438..3079)
                     /locus_tag="VFN42_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007400326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET8995951.1"
                     /translation="MLQQTVRIVKVISWNLLRRVGASVQDVALLISREQPDLLLMQEA
                     TRDVDELPARIGGHYARTPLPANSHGLAVWSRSPISFRVMDLPPGTLIRRVCQIVDLG
                     DFAVANVHLSHGQVLNRRQLRRIARVLPPRAAILGDFNLVGPALLAGFRDVGPRQPTH
                     IMGDIVPIRIDRCLARDITCTRARALSRETSDHSPILVDLDIAAAGVALAAAA"
BASE COUNT          552 a         1220 c         1331 g          550 t
ORIGIN      
        1 ccaggcgatc gcggccccca gccactcgcc gatggtgaag cgcctgggga agctgaaggc
       61 gaacagcggc gcggcattgt agatctgcca gccgctgccg atcatcgtca cgatggcgac
      121 ggcattcagc cagtgcatca gccgcaaggg ccacggatgc accgggcgga tacgggaaat
      181 tcgcgacatg acgctcctcc gctgctggcg cgcatgctcg cgccgtgccg acgcagggtc
      241 gaggcatcga atccataagc ggccggcatt ggacggcgtg acgcccccgc gccgacactc
      301 cgccgtgccg acagcggcgc cgcgccgcgc cggcatcgga atcccccacg ccgccgcgcc
      361 atgcgccggc cttcacggag ttggaatcat gacgaaatac ctgctcgtcg ccgccgccgc
      421 tgtcgcgctc gcgctggccc cggcggcgtt cgcccagacg gccgacatgt ccggcaacat
      481 gggccacgat ccggcgatgg cgcatggctg caaggccggc aaggacgcga tgggccatcc
      541 ctgcaagccc gacgccatgc ggcacgacac gatgggccat gacgcgatgg cgcatgagat
      601 gggccacgac gcgatgggcc acgccaccag cgccaactga gcggcgcgga ccgcgcttcg
      661 ctgcgtggcg gcgcgcgggt cttgcatccc cgcacgttct gccgtcactc tcgcggccgt
      721 cggaaaggca accgatgccc agtctgcagg agcaaaatct cggcgagcag ctccgcctcc
      781 gccgcctcgg ccagtccacc gaagtcaggg tggtctacga ggcgatggtg ggggagttgg
      841 cacgctccgg cttcatcgag catgtgctgc cggtcggcgc gcgcttcccc gacgtggcgc
      901 tgccgaccgc cgagggcgaa ctggcgcggc tggccgactg gtggcgccgg gggccgctgg
      961 tcgtcacgtt cttccgcggc gagtggtgcc cgtattgccg gctggtgctg gcggcgctgg
     1021 aggaagtgct gcccgagatc gaggcgctcg gcgcgaccct ggtcgcggtg acgccggaga
     1081 ccggcggccg ggcgctgacg gcgaagcggc gccacaacaa ccgctacgag atgctctcgg
     1141 atgtcgattg cggcctcggc ctcgattgcg gcgtggtgtt ccaggcgccc gccgcctatc
     1201 gggacatgct gctcaggttc ggcaacgatc tggccgaacg gcacggcaac gatgcctggt
     1261 tcctgccggt gccggcaacc ttcgtgctgg accgcgacgg catcgtgcgc tggcgcttcg
     1321 ccgaggtcga tttcacgaag cgcgcagaac ccgccgacat cctcgcggcc ttgcgggatc
     1381 ttgcggcgtc gtccgccggt tgaggcgagg gggcgatgga actcacccag gtccgctact
     1441 tcgtcgcctt gtgccggacg ctgaacttca cgcgcgcggc ggaaacctgc aacgtcactc
     1501 agcccgcgct gacccgcgcg atccagcggc tggaggacga actcggcggg ccgttgctct
     1561 atcgcgagcg caccctcacg cagctcaccg aactcggtcg cgccatgcgt ccgcatctgg
     1621 aagcgatgct ggaagcggcg gaggcggcgc ggcaggcggc gcgggcctgt gtcgggctgg
     1681 aggcggccag cgtgcgcatc ggcctcggcc cggccatcgc cgccgcccac gtcgccgggg
     1741 cgatccgtgg gctggtgcgg ctctatcccg aactctccat ccatttcgag gaggccggca
     1801 cggcggcgct cgcggaagcg atgctgtccg acatgctgga ctgcgcggtc ctgccggacg
     1861 atccgggcct gccggagcgg ctgaaccgct ggaagatcgc cgaggagggc tgcatcgtgg
     1921 tgctgccggg ggatcacgcg cttgctgcgc gcaccggcct gacggcggac gacctggcgg
     1981 gggagaccgt cctgcttggc gagcgctgcg gcgggtttgc gcatcggctg cgcgccgcgt
     2041 gcggcgatcg cttcgcgcag cggccgtgcg gcgggtcgtg ggtgcagatg ctcgatctgg
     2101 tccgggcagg gctgggcctc gccctgttgc ccgacggact ggcggtcggc ggtgggcttg
     2161 cggtccggcc cctggtcgag ccatgcctga ggcgcagcct gatgctcacg ctggtcgcag
     2221 gccggccgca cagcgcggcg gtggcgaacc tggtcaagct gtgccgggcg cggggaccgg
     2281 gagcggcgca gccggatgga gcgcgtaccc agcgccctgc ctgacggcgg cggcaccttt
     2341 cacgcaggct gacgcggcga cagccgctgg agagtgcccg ctcggggctt tgccggggcc
     2401 tctcatcgcc gccgacccgc cagcacggcc gcgggtttca ggcggcagcg gccagagcta
     2461 cgcctgcggc ggcgatgtcc aggtcaacga ggatggggga atggtccgag gtctcgcgcg
     2521 acagagcgcg ggccctggta caggtaatgt cccgcgcaag gcagcgatcg atccggatcg
     2581 ggacgatatc gcccatgata tgcgtcggct gacgcggacc gacatcccgg aagccggcaa
     2641 gcagcgccgg gccgaccaga ttgaaatcgc cgaggattgc ggcgcgcggc ggcaggaccc
     2701 gggcgatccg ccgcaattgg cggcggttca gtacctgccc atgcgagaga tggacgttgg
     2761 cgacggcgaa atcccccagg tcgacgatct ggcagacacg gcggatgagg gttccaggcg
     2821 gcaggtccat gacccggaag ctgatggggg acctgctcca cacggcaagg ccatgactgt
     2881 tcgcgggcag gggagtccgc gcatagtgcc cgccgatcct ggctggcagc tcgtcaacgt
     2941 cccgcgttgc ctcctgcatc aacagcaggt cgggttgctc tcgggatatc agcagggcca
     3001 cgtcttgcac cgaggcacca acccggcgca acagattcca actaataacc ttcacaatcc
     3061 gaaccgtttg ttgcagcagc ctgtacgcgc agcgtcgcca cttggttgtc gcttctcgat
     3121 gagaacagca cagcccgcgc tgcctctaac ggattcgcga ccggttgtcc accgcgaggg
     3181 gtggcgctgc ccggtcaagg ggaagcggct tggcgtcgat gtcccggcgg tggacgtcga
     3241 tgccgccctc cttgccgggc ccgctggagg cggtccggcg ccatgatgtc cctggcggcg
     3301 ttccggtcgc catcctgtga tccaccagat gcgtgccgct tgatccgcgt caaggcgtgg
     3361 agttgccggg gacgcactct gaaaaccgcg atggatctgg ccggaatgcc atgcatggcg
     3421 cggggcggcg gcaagcatgg cggctccggc cattgatgtc gtccaagccg ggaaggagtg
     3481 ccggactgtc gccgcactgc cgggaataac ggcccgcgac gggagaggga catgcgattg
     3541 atccatgaag tgatcgagga tttgcaggcg tgggaggcgg cccagacctc ggaggatgag
     3601 gatcggaccc ccttggccac cgtcctggag gaattgcttg gcggtgagcg tgg
//
LOCUS       DASSZH010000219         3690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_516389, whole genome shotgun sequence.
ACCESSION   DASSZH010000219 DASSZH010000000
VERSION     DASSZH010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516389"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VFN42_04730"
     CDS             complement(<1..801)
                     /locus_tag="VFN42_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HET8995952.1"
                     /translation="MPINPADGPILGTLYGSDAMRAVFDERSYFQRMLDVEAALARVQ
                     ARLGIIPAEAASAITEAARFENLRADELAASARNVGYPVVGLVNGLSRAAGAAGGWTH
                     WGATTQDIMDTATVLQVRDGLALVRTELAALLRALSTQADRHRRTVMAGRTHLQQALP
                     TSFGLKCATWALPFVSHLQRLDQLRPRVELVEFAGAAGTLASLGDQGIAVMEALAVEL
                     ALGVPVAPWHVSRDGLAETVSFLGLVCGSLAKLATDIILLAQTEVGEVA"
     gene            complement(891..1262)
                     /locus_tag="VFN42_04735"
     CDS             complement(891..1262)
                     /locus_tag="VFN42_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8995953.1"
                     /translation="MPEGPERPRVLVVEDEAMIAMLIEDMLEELGYAVAMVAGRLQTA
                     LQAVEAHNFDVAIVDVNIAGQTSYPVADRLTARGIPFAFVTGYGSNGLAEAYAAIPVL
                     QKPFRIADLTQLMQRLLTGAG"
     gene            complement(1268..2677)
                     /locus_tag="VFN42_04740"
     CDS             complement(1268..2677)
                     /locus_tag="VFN42_04740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HET8995954.1"
                     /translation="MDIAATLEPMPATATPDRHAERRADDHQAMLQAVFDTTPECIKV
                     VAPDGSLLEMNRAGLEMIGAASLDAVRGACVFDMIAPEDREAWRQRHDRVCRGEHLSW
                     SFDLVLQHGLRRHMETHAAPFPMPDGSTAHLAVTRDITERRRDRQRLEESERRLRDLL
                     DALPAAIYTTDRAGRLTFYNQAAALLAGCEPRLGEDSWCVAWRLLDSEGAPIPHDQCP
                     MAITLKQQQAVRGTEVMAERPDGQRVPILPYPALLRDAAGAVVGGVNMLVDISARKDA
                     ERRQKLLVNELNHRVKNTLATVQSIAAQTFRGLADTPARQWFDARLLALSQVHDILNR
                     ENWEGADLGDIILQTIAPLGDPQRNRFTLDGPELRLPPGMALSVAMALHELCTNAAKY
                     GALSGAAGQVAITWSAEPHRRLRFRWEESGGPPVRPPAARGFGSRLIERGLARELGGS
                     ARLDFAPSGVLCEIDAPLP"
     gene            complement(2848..>3690)
                     /gene="dnaA"
                     /locus_tag="VFN42_04745"
     CDS             complement(2848..>3690)
                     /gene="dnaA"
                     /locus_tag="VFN42_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET8995955.1"
                     /translation="EGGRRAKPLSVAYMSAEKFMYRFIAAIRSQSTMEFKEQLRSVDV
                     LMVDDLQFLIGKDNTQEEFFHTFNALVDAGKQIVVSADKSPAEISGLEDRLRTRLGCG
                     MVADLHATTYELRISILESKAQRAGVTVPGKVQEFLAHKITTNVRELEGALNRLIAHA
                     NLFGRPVTLESTQEVLHDILKAHDRRVTIEEIQRKVAEHYNMRLTDMSSARRARAVAR
                     PRQVAMYLAKQLTSRSLPEIGRKFGNRDHTTVMHAVSRIGELIERDAGFAEDVELLRR
                     MLES"
BASE COUNT          556 a         1258 c         1259 g          617 t
ORIGIN      
        1 ggccacctcg ccgacctcgg tctgcgccag caggatgatg tcggtggcca gcttggccag
       61 gctgccgcag accaggccaa ggaagctcac cgtctcggcg agcccatcgc ggctgacatg
      121 ccagggcgca accggcacgc caagcgcgag ttcgacggcc agcgcctcca tgacggcgat
      181 gccttgatcg ccgagcgagg ccagcgtgcc ggcggcgccg gcgaactcca ccagctcgac
      241 gcgtgggcga agctggtcca gccgctgcag gtgggacacg aagggcagcg cccaggtggc
      301 gcatttcagt ccgaagctgg tgggcagcgc ctgctgcaga tgcgtgcggc cggccatcac
      361 ggtccgccgg tgccggtctg cctgcgtgct cagcgcgcgc agcagcgcgg ccagttcggt
      421 tcgcaccagc gcaaggccgt cgcgcacctg cagcacggtc gcggtatcca tgatgtcctg
      481 cgtggtcgcg ccccaatgcg tccagccacc cgcggcgccc gcggcacgcg acaggccgtt
      541 gaccaggccg accaccggat agccgacatt gcgcgcgctg gcggcgagtt catcggcacg
      601 gagattctcg aaccgtgcgg cttcggtgat tgcggatgcg gcttcggcgg gaatgatgcc
      661 gagccgcgcc tgtacccggg ccaaggcggc ttccacgtcc agcatgcgct ggaagtagct
      721 gcgctcgtcg aacaccgcgc gcatcgcgtc gctgccgtag agtgtgccga ggatcggacc
      781 gtcggccgga ttgattggca tggttgggct ccaactgcgt gcgacgaaac tcgcatggcc
      841 agaggcggcg cccaagcccg gccgcgcatg gttgcctgtg cgtgatcccg ctacccggcc
      901 ccggtcagca ggcgctgcat caattgcgtc aggtccgcga tgcggaacgg cttctgcagc
      961 accgggatcg cggcataggc ttccgccagg ccgttgctgc cgtagccggt gacgaacgcg
     1021 aaggggatcc cgcgcgccgt caaccggtcg gctaccgggt agctggtctg cccggcgatg
     1081 ttcacgtcca cgatcgccac gtcgaaattg tgcgcttcca ccgcctgcaa tgccgtttgc
     1141 agccggccgg caaccattgc gaccgcgtag ccgagttcct ccagcatgtc ctcgatcagc
     1201 atggcgatca tggcttcgtc ctcgaccacc agcacccgcg gccgttccgg cccttccggc
     1261 atcgcgttca cggcaatggc gcgtcgatct cgcacagcac gccgctcggc gcgaagtcga
     1321 gtcgcgccga tccgcccagt tcgcgcgcca gcccgcgctc gatcagccgc gaaccgaagc
     1381 ccctggcggc gggcggccga accggcgggc cgccgctctc ctcccatcgg aagcgcagcc
     1441 ggcggtgcgg ttccgccgac caggtgatgg cgacctggcc ggcagctcca ctcagggcac
     1501 cgtatttcgc ggcgttggtg caaagctcat gcagggccat ggcgaccgac aatgccattc
     1561 cgggcggcag ccgcaattcg ggaccatcga gcgtgaaacg gttgcgctgc ggatcgccaa
     1621 gtggggcaat cgtctgcagg atgatgtcgc cgaggtcggc gccttcccag ttttcccgat
     1681 tgaggatgtc gtgcacctgg gacaacgcca gcagccgcgc atcgaaccac tggcgcgccg
     1741 gcgtatcggc caggccgcgg aaggtctgcg cggcgatcga ctgcactgtc gccagcgtgt
     1801 tcttcacccg gtgattgagt tcgttcacca gcagcttctg gcggcgttcc gcgtccttgc
     1861 gggcggagat gtccaccagc atgttcaccc cgcccaccac ggcgccggcg gcatcgcgca
     1921 gcagcgcggg atagggcagg atcggcacgc gctgcccgtc cggccgttcc gccatcacct
     1981 cggtcccacg cacggcttgc tgctgcttca gggtgatcgc catggggcat tggtcgtgcg
     2041 ggatcggggc gccttcgctg tcgagcagcc gccaggcgac gcaccagcta tcctcgccga
     2101 ggcgcggttc gcacccggcc agaagcgcgg ccgcctggtt gtagaaggtc agccggccgg
     2161 cgcgatcggt ggtgtagatc gccgcaggca gtgcatcgag caggtcgcgc aggcggcgtt
     2221 cgctttcctc cagccgctgc cggtcgcgcc ggcgttcggt gatatcgcgc gtgacggcga
     2281 ggtgcgccgt gctgccgtcc ggcatgggaa agggcgccgc atgcgtctcc atgtgccgcc
     2341 gcaggccgtg ctgcaggacc aggtcgaacg accaggagag gtgttcgccg cgacagacgc
     2401 ggtcgtgccg ttgccgccag gcctcgcgat cctccggcgc gatcatgtcg aacacgcagg
     2461 cgccgcgcac cgcgtccagg ctggccgcgc cgatcatctc cagcccggcg cgattcatct
     2521 ccagcaggct gccgtcaggt gcgacgacct tgatgcattc cggtgtggta tcgaaaaccg
     2581 cctgcagcat ggcctggtga tcgtccgcac ggcgctcggc gtgccggtcc ggcgttgcgg
     2641 tcgccggcat gggctccagg gtggctgcaa tgtccatcgg cataaacacg tcctccgcga
     2701 ccggcgggcc ggcgagacaa tcgggtctcg caacaggtcc gccgctcctg cggaatcacc
     2761 acgtcagcgg gcattttgga cagacagtct ctccggaacg tggctggccg cacggggatg
     2821 tgaatccgtg cggattgcct ggccggatca gctctccagc atgcggcgca gcagctcgac
     2881 gtcctcggcg aagccggcgt cgcgctcgat cagttcgccg atgcgcgaga ccgcatgcat
     2941 cacggtggtg tgatcacgat tgccgaactt gcggccgatc tccggcaggg agcgactggt
     3001 cagctgcttg gcgaggtaca tcgcgacctg gcgtgggcgc gccaccgcac gggcgcgccg
     3061 cgcggacgac atgtcggtta ggcgcatgtt gtagtgctcc gccaccttgc gctggatctc
     3121 ctcgatcgtg acgcgacgat cgtgcgcttt caggatgtcg tgcagcactt cctgggtgga
     3181 ttccagcgtc accggacgtc cgaacaggtt ggcatgcgcg atcaaccggt tcagcgcgcc
     3241 ttccagctcc cgcacattgg tggtgatctt gtgggcgagg aattcctgca ccttgccggg
     3301 cacggtcacg ccggcgcgct gtgccttgga ttccaggatg gagatgcgca gctcgtaggt
     3361 ggtggcatgc aggtcggcga ccatgccgca gcccaggcgg gtgcgcagcc ggtcttccag
     3421 ccccgagatc tccgccggcg acttgtcggc cgagaccacg atctgcttgc cggcatcgac
     3481 cagcgcattg aaggtgtgga agaattcctc ctgcgtgttg tccttgccga tgaggaactg
     3541 caggtcgtcc accatcagca cgtccacgct gcgcagttgc tccttgaact ccatggtgga
     3601 ctggctgcgg atggcggcga tgaagcggta catgaacttc tcggccgaca tgtaggcgac
     3661 cgacagcggc ttggcgcggc gtccgccctc
//
LOCUS       DASSZH010000220         2615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1505235, whole genome shotgun sequence.
ACCESSION   DASSZH010000220 DASSZH010000000
VERSION     DASSZH010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505235"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..1763
                     /gene="istA"
                     /locus_tag="VFN42_04750"
     CDS             240..1763
                     /gene="istA"
                     /locus_tag="VFN42_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004666649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HET8995956.1"
                     /translation="MRQVREVLRLKHVCGQSARQIAAAVGVSRYTVAEYLRRAGVVGI
                     TWPVPPEFDDAALERRLFTSPGIIAPEKLRPPPDWARLHGELRRPGVTLLLLWEEYRA
                     GQPEGYGYSRFCDLYAAWRGRLSPTMRQSHPAGERLFVDYAGQTVDVIDAITGEVRAA
                     QIFVAALGASNLTYAEARWTQGLADWLACHVNTFAFVGGAARQLVCDNLKAGVTATCR
                     YEPGINRSYQDLAAHYGTAVVPTRVRKPRDKAKVEVAVQVVQRWVLARLRHRRFFSLA
                     ELNAAIRDLIADLNARPMRHLGTSRRALFEAIERAALLPLPGEPYVYAEWRRCRAGID
                     YHVEVHGHWYSVPSRLVREVIDARITEQTVELFHRGTRVASHLRSPQRGRHTTTPEHM
                     PSAHRRYADWTPARLMREATAIGPSTAALVERILHAKPHPEQGFRACLGILRLVRAWG
                     AERVEAACQRGMDIGARSYSSIVSILRNNLDRAYRPQPVPDEPPVQHSNIRGGRYYH"
     gene            1777..2541
                     /gene="istB"
                     /locus_tag="VFN42_04755"
     CDS             1777..2541
                     /gene="istB"
                     /locus_tag="VFN42_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HET8995957.1"
                     /translation="MLSHPTGDRLRELGLIGMARALEEMRRQPDSAALGFEDRLALLV
                     DRELSERDTKRLHARLRFAGLRIQATPEDVDYRAARGLDRALFQKLTRGEWIERHENV
                     LITGPTGTGKTWLSCALGHRACRDNRSVLYHRVPRLLENLGLARGDGRYARMLKSLAR
                     VNLLILDDWGITPLTAEQRRDLLEIVDDRHGRASTVVTSQLPVAHWHEHIGNPTIADA
                     VLDRLVHTAQRIDLKGESLRKLHAGKAAKLDEATAS"
BASE COUNT          420 a          938 c          859 g          398 t
ORIGIN      
        1 gatttttgca ccggatttct gcgacgagaa aggcgtgtgg taacctgcgc attccgacgc
       61 aaagcagccg ggcattccga tggaagccgg ccggtgattc cgacacaagc cagccaccca
      121 ttccgaggca agccagccac cctggagggg tgaacggcgc gacctgaggg cagtcatcaa
      181 ccgacggcag gctgctttcc cacagcaggg gaagcgccat gccagccgag agactgccga
      241 tgcgtcaggt tcgagaggtc cttcgtctca agcacgtctg cgggcagagt gctcggcaga
      301 ttgccgcggc ggtgggcgtc agccgctaca cggttgccga gtatctgcgc cgggctggcg
      361 tggtgggcat cacctggccg gtgccgccgg agttcgacga cgcggcgctg gagcgccgtc
      421 tgttcacgtc gccgggcatc atcgcaccgg agaagctgcg cccgccgccg gattgggcgc
      481 ggctccatgg cgagctgcgc cgtcccggcg tgacgctgct gttgctgtgg gaggaatacc
      541 gggcggggca gccggagggc tacggctaca gccggttctg tgacctctat gccgcgtggc
      601 gcgggcggct gtcgccgacg atgcggcaga gccatcctgc cggggaacgg ctgttcgtgg
      661 actatgccgg acagacggtc gatgtcatcg acgcgatcac cggcgaggtg cgggcggcgc
      721 agattttcgt tgccgccctt ggcgcctcca acctgaccta tgcggaagcc cgctggacgc
      781 agggactggc ggactggctt gcctgccacg tcaacacgtt cgccttcgtc ggcggcgccg
      841 cgcggcaact ggtgtgcgac aatctcaagg cgggcgtcac cgcgacctgc cgctacgagc
      901 ccgggatcaa ccgcagctat caggacctgg cggcgcacta cggcaccgcc gtggtgccga
      961 cgcgggtgcg caagccacgt gacaaggcca aggtcgaggt cgccgtgcag gtggtgcagc
     1021 gttgggtgct ggcgcggctg cgacatcgcc gcttcttctc gctggccgaa ctgaacgccg
     1081 cgatccgcga cctgatcgct gacctcaacg cccgcccgat gcgccatctc ggcaccagcc
     1141 gtcgtgcgct gttcgaggcg atcgagcgcg ccgccctgct gccgctgccc ggcgaaccgt
     1201 atgtctatgc cgaatggcgg cgctgccgtg cgggcatcga ctaccacgtc gaggttcacg
     1261 gccattggta ttccgtcccc tcccggctgg tgcgcgaggt gatcgacgcg cgcatcaccg
     1321 agcagaccgt ggagctgttc caccgcggca cccgcgtcgc cagccatctg cgcagcccgc
     1381 agcgcgggcg gcacaccacc acgcccgagc acatgcccag cgcgcaccgc cgctacgccg
     1441 actggacgcc ggcgcgactg atgcgcgagg cgaccgcgat cggcccgtcc actgccgccc
     1501 tcgtcgagcg gatcctgcac gccaagccgc atcctgaaca gggcttccgc gcctgcctcg
     1561 gcattctgcg gctggtgcgc gcgtggggcg ccgagcgcgt cgaggccgcc tgccagcgcg
     1621 gcatggatat cggcgcccgc agctacagct cgatcgtctc gatcctgcgc aacaacctcg
     1681 accgggccta ccgcccgcag ccggtgccgg acgaaccgcc cgtccagcac agcaacatcc
     1741 gtggcggccg ctactatcat tagagggaga ccacccatgc tcagccatcc caccggtgac
     1801 cgtctgcgcg aactcggcct gatcggcatg gctcgcgcac tggaggagat gcgccgccaa
     1861 cccgacagtg ccgcgctcgg cttcgaggac cggctggccc tgctggtcga ccgcgaactg
     1921 tccgagcgcg acaccaagcg cctgcatgcc cgcctgcggt tcgccggctt gcgcatccag
     1981 gcgacaccgg aagatgtcga ctaccgtgcc gcccgtggcc tcgaccgtgc gctgttccag
     2041 aagctgaccc gcggcgagtg gatcgaacgc catgagaatg tgctgatcac cggcccgacc
     2101 ggcaccggca agacctggct ctcctgtgcg cttggccatc gcgcctgtcg tgacaatcgt
     2161 tcggtgctct accaccgggt gccgcgcctg ctcgaaaacc tgggcctggc ccgcggcgac
     2221 ggtcgctatg cccgcatgct caagagcctc gcgcgggtga acctgctgat cctggacgac
     2281 tggggcatca ctccattgac cgccgaacag cgccgcgacc tgctggagat cgtcgatgac
     2341 cgacacggcc gcgcctccac cgtcgtcacc agccaactgc cagtcgcaca ctggcacgaa
     2401 cacatcggca acccgaccat cgccgatgcc gtgctcgacc gcttggtgca tacggcgcag
     2461 cgcatcgacc tgaaaggcga gtcattgcgc aaactgcatg ccgggaaggc cgccaagctt
     2521 gacgaggcga ccgcctcctg agccaaatgc caacgccctc agaccgcgcc accgccccgg
     2581 ccggcttcca tcggaatgcc cggctggctt gcctc
//
LOCUS       DASSZH010000221         2410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_942778, whole genome shotgun sequence.
ACCESSION   DASSZH010000221 DASSZH010000000
VERSION     DASSZH010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942778"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1200)
                     /locus_tag="VFN42_04760"
     CDS             complement(1..1200)
                     /locus_tag="VFN42_04760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8995958.1"
                     /translation="MKFGIFYELQLPRPWEPGDELRLYQNALTQVELADRLGYDYAWE
                     VEHHFLEEYSHSPAPEVFLAAASQRTKNIRLGHGIVQLTTNPPQRVAERIACLDLVSN
                     GRVEFGTGESASITELGGFGRDMETKREIWEDALRCIIPMFQDKGVALDTKWHQMPMR
                     NVLPKPVQKPHPPLWVACSQLETIEMAGKRGLGALGFQFLSADAAHAWVHAYYNAFVK
                     RQEKLAEYVTNPNIALVSYFMCAETDEEARRRADGATFFQFALRYYGASSGRKRPDPY
                     TVNMWDEYNTWKRENPEAAARALSGGLIGSPETIRRKLRRFQTSNIDQVILLNQAGRN
                     QHEHICESRELFAREVMPDFHAQIPAHEEWKRQVLAGKIELEEIDTQPFRDRFGPNTV
                     QVAQAAE"
     gene            1446..2051
                     /locus_tag="VFN42_04765"
     CDS             1446..2051
                     /locus_tag="VFN42_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007399154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET8995959.1"
                     /translation="MHFLPNERIALFIDGANLYSASRNLGFDVDYRNLLEFFRRKAQV
                     VRAYYYSAVLETDEYSPLKPLTDWLAYNGYSLVTKPAKEFTDSTGRRRVKGNMDIEVA
                     VDMLELAPRIDHAVLFSGDSDFRRLVEAVQRKGVRVSVVSSIRTAPPMVADELRRQAD
                     QFLELAEIAPEFTRRQTEPRVRAVPLRHTPAEPFADPNDAA"
     gene            2048..>2410
                     /locus_tag="VFN42_04770"
     CDS             2048..>2410
                     /locus_tag="VFN42_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET8995960.1"
                     /translation="MTTAAAPAAPAPDCPLCPRLLEYRLANRAANPDWFNAPVPSFGG
                     LDARLLVVGLAPGVRGANRTGRPFTGDYAGVLLYQTLLRFGLAAGTYAATPADGLRLL
                     GCRVTNAVRCVPPGNLPTP"
BASE COUNT          362 a          811 c          783 g          454 t
ORIGIN      
        1 tcattccgcc gcctgcgcca cctgcaccgt gttgggtcca aaacgatcac ggaacggctg
       61 cgtgtcgatc tcctccagtt cgatcttgcc ggccagcacc tggcgcttcc attcctcgtg
      121 cgccgggatc tgggcgtgga agtccggcat cacctccctg gcgaacagct cccggctctc
      181 gcagatatgc tcatgctggt tgcggcccgc ctggttcagc aggatcacct ggtcgatgtt
      241 ggaggtctgg aaccggcgca gcttgcggcg gatggtttcc ggcgaaccga tgagcccgcc
      301 cgacagcgcc cgcgccgcgg cttccgggtt ctcccgcttc cacgtgttgt actcgtccca
      361 catgttcacc gtgtacgggt ccggccgctt gcgcccggaa ctggcgccgt aatagcgcag
      421 cgcgaactgg aagaacgtcg cgccatccgc tcgacggcgc gcttcctcat cggtctcggc
      481 gcacatgaag tagctgacca gcgcgatgtt cgggttggtg acgtactcgg ccagcttctc
      541 ctgccgcttc acgaaggcgt tgtaataggc gtgcacccag gcatgtgccg cgtcggcgga
      601 caggaactgg aagcccagcg cgcccaggcc gcgcttgccg gccatctcga tcgtctccag
      661 ctggctgcac gccacccaga gcggcggatg cggcttctgc accggcttgg gcagcacgtt
      721 gcgcatcggc atctggtgcc acttggtgtc cagcgccacg cccttgtcct ggaacatcgg
      781 gatgatgcag cgcagcgcgt cttcccagat ctcgcgcttg gtctccatgt cgcggccgaa
      841 accgcccagc tcggtgatcg acgccgattc gccggtgccg aattcaaccc ggccgttgct
      901 caccaggtcc aggcaggcga tgcgttcggc gacgcgctgc ggcgggttgg tggtcagttg
      961 cacgatgccg tggccgagcc ggatattctt ggtccgctgg ctggcggcgg cgaggaagac
     1021 ctccggggca ggggagtgtg agtattcctc caggaaatga tgctcgactt cccacgcata
     1081 gtcgtatccc agccgatcgg cgagttcgac ctgggtcagg gcgttctggt acagccggag
     1141 ctcgtcgccc ggctcccagg gccgcggtag ctgcagctcg tagaagatgc cgaacttcat
     1201 gggacgtttc ctttgctact ggcgagtagt gcgccgcagc atgccgatgc tgtcaagccg
     1261 agtgccgggt gcagtggccg cttcggtccg cacgtgaccg gtttcacttt cctgcaatat
     1321 cggcgatgct ggcggccctc gccaaagcga ggctgattcc gtcattgccc gttctgggcc
     1381 tttacccctg gtgttgccga acctgatccg gacgcgacaa tcctgtttga agagaggacg
     1441 acctcatgca tttcctgccc aacgagcgta tcgcattgtt tatcgacggc gccaatctct
     1501 actctgcttc ccgcaatctt ggtttcgatg tcgactaccg caatctgctg gagttcttcc
     1561 gccgcaaggc gcaggtggtg cgggcctatt actactcagc cgtactggaa actgacgaat
     1621 actccccgct gaagccgctg accgactggc tcgcctataa cggctacagc ctggtgacca
     1681 agccggccaa ggagttcacc gactcgaccg gccgccgccg ggtgaagggc aacatggata
     1741 tcgaggtcgc ggtggacatg ctggaactgg cgccgcgcat cgatcacgcc gtcttgttca
     1801 gcggcgattc cgacttccgc cggctggtgg aggcggtgca gcgcaaaggg gttcgggtgt
     1861 cggtggtctc ctcgatccgc acggcaccgc cgatggtggc ggatgagttg cggcggcagg
     1921 ccgaccaatt tctggaactg gccgagatcg cccccgagtt cacccgccgg cagaccgagc
     1981 ctcgggtgcg cgccgtgccg ctgcgccaca cgcccgccga acccttcgcc gacccgaacg
     2041 acgcggcgtg accacagccg cagcgccggc cgctccggcg cccgactgcc cgttatgccc
     2101 gcggctgctg gaataccggc tggcgaaccg cgcggcgaac ccggactggt tcaacgcgcc
     2161 ggtgccgagc ttcggcgggc tggacgccag gctgctggtc gtggggctgg cgcccggcgt
     2221 gcgtggggcc aaccgtacgg gccggccctt caccggcgac tatgcgggcg tgctgctgta
     2281 ccagacattg ctgcgcttcg ggctggccgc cggcacctat gccgccacgc cagccgacgg
     2341 gctgcggttg ctcggctgcc gggtgacgaa tgcggtgcgc tgcgtacccc cgggcaacct
     2401 gccgacaccg
//
LOCUS       DASSZH010000222         4539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1130098, whole genome shotgun sequence.
ACCESSION   DASSZH010000222 DASSZH010000000
VERSION     DASSZH010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130098"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFN42_04775"
     CDS             <1..205
                     /locus_tag="VFN42_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995961.1"
                     /translation="FARNTGLPHFMTQAWLTGRFEPSATRVAMHLDRQPNHLFGPFPR
                     NRHPLRLLWPTVALLLFSVVKQQ"
     gene            1010..3397
                     /locus_tag="VFN42_04780"
     CDS             1010..3397
                     /locus_tag="VFN42_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HET8995962.1"
                     /translation="MDGDIARLTAIRRYAVPGAAFGEAPLPARALDPGMGLAVGRRTV
                     FRPDDQECYGRVADRVAAGNNALLGRHDPATAIEQARLRNAIATGALLTSGRHLQHGD
                     LDQPTRNMEVFTNCATAACSFTKFYLLLNGSGVGRSYDDALMAVDWTNAPELLLYLSP
                     AHPDHPAASGDRARFARELGISVEQVDTFLARELLPAPGAAPAGADYYRIDDSREGWA
                     KSVELLEAMAFRGERGRTLVLDLSAIRPAGSPIRGMQGRPASGPVSLLRAFLNIRREV
                     IEPARRRDPDGEAMQPWEQALRLDHHLSVEVQVGGARRAARMATKSWRDPGVLRFIGL
                     KEMGGLWTANNSVMVDAEFWSRVRQGDPADPLTRHAVAVFQEATRCAWVNGEPGFING
                     DRLEDHRTGVARQRPVDSTGSGFRSGSYQVESGAPLLAELTRRAAVSDFPATTNPCGE
                     ITLHVTGGYCVIADFAPLLACPVPLDDMVPGAADPATAALWDARVEDAVRLGVRFLMR
                     ANTMDSLYAAEVERTNRMGIGPTGLHEYAWMRFGLTFHDLLDERKAAPFWAMLDRLSQ
                     AAKDEGGTYAAELGLRRLVTVTTVKPSGTISKLFGLSEGAHLPARAQYLRWVQFKGTR
                     DPDTGAWRPGSDPLLADYEARGYPLRTLTTFPGITIVGFPTVPLIMRLGLADRLVTAP
                     EATPEQQYRWLRLLERYWIGDEQGNQVSYTLKLYTDRHDLDGFRDIVLREQPEVRCCA
                     ILPTRPDHALGYEYLPEEELPADAFAELVAAIDAAEAREAVDMVHLQCASGVCPI"
     gene            3483..3842
                     /locus_tag="VFN42_04785"
     CDS             3483..3842
                     /locus_tag="VFN42_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET8995963.1"
                     /translation="MGIWNPFRRHSAAEPAWVDAAELRQRIASGTPPLILDVRQPEEF
                     TAPPGHLPGALNLPLGQVAQQTAQLSARRQAVVVVCKTDRRSARAAAELCAAGLQDVS
                     VLRGGTDGWHRLGLPLE"
     gene            complement(3861..>4539)
                     /locus_tag="VFN42_04790"
     CDS             complement(3861..>4539)
                     /locus_tag="VFN42_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8995964.1"
                     /translation="FTWEEGVDKHPVGACLEIIDHLTHNVYAGRMAHWAAFYERIFNF
                     REIRYFDIKGEYTGLFSRALSSPDGKIRIPINESADARSQIEEFLHAYNGEGIQHIAC
                     GCRDIHATVERLRGDGLEFMPSPPDTYYARVDERVPGHGEDLQRLRRNGILVDGEGAV
                     ALGETAGRKTRLLLQIFSATLVGPIFFEFIQRKGDEGFGEGNFRALFVSIEEDQLRRG
                     VLTRDAA"
BASE COUNT          706 a         1690 c         1481 g          662 t
ORIGIN      
        1 gttcgcacgc aataccggcc tgccgcactt catgacacaa gcatggctca caggccgatt
       61 cgagccatcc gcgacccgtg ttgcgatgca cctcgatcgc cagcccaatc atctgttcgg
      121 tccgttcccc cgcaatcggc atccgctccg actcctgtgg cccaccgtgg ccctcctgct
      181 tttctccgtg gtaaaacaac aataaatcgt aaacgcacga gcccagcttt attcccccgg
      241 accggccata tcgacggccc ggccagcgcc aaacggccgc tccccaccgc atcccaacgt
      301 ctccgcgcca tgatcagggt cgaagaagcc ggcatccgcc ttctcgcgga cctgcgcccc
      361 accccggcgg acatcgtcgc ccccgcggaa gcccggagca gaggggccgg gccacaagtc
      421 accgcgcgcc ggggtgagag gcgtgcgcca catgcgctgg gttttggatg gcaggatggt
      481 tcgagatgcc ggctccccac ccaaagcggc cacccaggga aggcgtactc ggcagccgag
      541 tgccgcaggc cgatggagcc gcgaaaccgc tatgccgccg ccgcaaccgg tcaaaacctt
      601 tgtgccgagc tactagacaa ggcgaccggc catcaccatc tgccggtccg tctaagtcgt
      661 cccccgtaca ccgcacgcgc ccgaccgcac gtgcagcacc gaacggaagg cgtgccggct
      721 gggtggcccc cggccttccg tgtgtcgttg caggacagcc ctgcggtccc gcgaccggca
      781 cagcgcccca aggtgccaat cctgacgcgc ctgcgcccaa ccgccccgtc gcgccgccat
      841 gcgccgccgc caacccggcg accgggaccg cacgccggca ggcagccgtg cggtccggcc
      901 gcgccggccc gccccggatt tcgccgcacc gcccggtatc tagttgcgca tggcccccaa
      961 tcggcataga tatggtgtcc accacgactc gacaggggca gcgacgacca tggatggaga
     1021 catcgcccgg ctcaccgcca tccgtcgcta cgctgtgccc ggcgccgcct tcggagaggc
     1081 gccgttgccg gcccgcgccc tcgatcccgg catgggcctc gccgtcggcc gccgcaccgt
     1141 gttccgcccg gacgaccagg agtgctacgg ccgcgtcgcg gaccgcgtcg ccgccggcaa
     1201 caacgccctg ctcggccgcc acgatcccgc caccgccatc gagcaggccc gcctgcgcaa
     1261 cgccatcgcc accggcgcgc tactcacctc cggccggcat ctccagcacg gcgacctcga
     1321 tcagccgacc cgcaacatgg aggtgttcac caactgcgcg acggccgcct gttccttcac
     1381 caagttctat ctgctgctga acggctccgg cgtcggccgc agctatgatg atgcgctgat
     1441 ggcggtcgac tggacgaacg cccccgaact gctgctgtac ctctcgccgg cgcatcccga
     1501 ccatccggcc gcctccggcg accgcgcccg cttcgcccgc gagcttggca tcagcgtgga
     1561 gcaggtggac accttcctcg cccgcgaact gctgcccgcc cccggtgccg cgccggccgg
     1621 cgccgattac taccgcatcg acgacagccg cgaaggctgg gccaagtcgg tcgaactgct
     1681 ggaagcgatg gccttccgcg gcgagcgcgg tcgtaccctg gtgctcgacc tgtcggcgat
     1741 ccgcccggcc ggcagtccga tccgcggcat gcagggccgc ccggcctccg gcccggtgtc
     1801 gctgctgcgc gccttcctca acatccggcg cgaggtgatc gagccagcgc gccgtcgcga
     1861 cccggatggc gaggccatgc agccctggga gcaggcgctg cggctcgacc accatctgtc
     1921 ggtggaagta caggtgggcg gcgcccgccg cgcggcgcgc atggcgacca agtcgtggcg
     1981 cgacccgggc gtgctgcgct ttatcgggtt gaaggaaatg ggcgggctgt ggaccgccaa
     2041 caactcggtg atggtcgatg ccgagttctg gtcgcgcgtc cgccagggcg atcccgccga
     2101 tccgctgacc cggcatgccg tcgcggtgtt ccaggaagcc acccgctgcg cctgggtcaa
     2161 cggcgagcct ggcttcatca acggcgaccg gctggaggac caccgcaccg gcgtggcccg
     2221 ccagcgtccg gtggacagca ccggcagcgg cttccgcagc gggagctacc aggtggaatc
     2281 cggcgccccc ctgctggcgg aactgacccg ccgcgccgcc gtctcggatt tccccgccac
     2341 caccaatccc tgcggcgaaa tcacgctgca cgtcaccggc ggctactgcg tcatcgccga
     2401 tttcgcgccg ctgctggcct gcccggtgcc gctggacgac atggtccccg gcgccgcgga
     2461 tccagcaacc gcggcactgt gggacgcgcg ggtggaggac gcggtgcgcc tgggcgtgcg
     2521 cttcctgatg cgcgccaaca ccatggactc gctgtacgcg gcggaggtcg agcgcaccaa
     2581 ccgcatgggc atcggcccga ccggcctgca cgaatacgcc tggatgcggt tcggcctgac
     2641 cttccacgac ctgctggacg agcgcaaagc cgcaccgttc tgggcgatgc tggaccggct
     2701 gtcgcaagca gcgaaggacg agggtggcac ctatgccgcg gaactcggcc tgcgccgcct
     2761 ggtgacggtg accacggtga agccgtccgg caccatcagc aagctgttcg gcctgagcga
     2821 aggcgcgcat ctccccgccc gagcgcagta tctgcgctgg gtgcagttca agggcacgcg
     2881 cgatccggac accggcgcat ggcggccggg ctccgacccg ctgctcgcgg actacgaagc
     2941 gcgcggctac ccgctgcgca cgctgacgac cttccccggc attaccatcg tcggcttccc
     3001 caccgtgccg ctgatcatgc ggctgggcct cgccgaccgg ctggtgaccg cgccggaagc
     3061 cacgccggaa cagcaatacc gctggctgcg actgctggag cgctactgga tcggcgacga
     3121 acagggcaac caggtcagct acacgctgaa gctgtacacc gaccggcacg acctggacgg
     3181 gttccgcgac atcgtgttgc gcgagcagcc ggaggtgcgc tgctgcgcca tcctgccgac
     3241 gcgtccggac cacgcgctgg gttacgaata cctgccggag gaagaactgc cggccgacgc
     3301 cttcgccgaa ctggtggcgg cgatcgatgc ggcggaagcc cgcgaggcgg tggacatggt
     3361 gcacctgcaa tgcgccagcg gggtgtgccc gatttagccg ccggcacgcc tgcacgatcg
     3421 ccagcttccc atcccggcgc gcctgccgta atccgatcct gcgtcagcag ggaccggtcg
     3481 acatgggcat ctggaatccg ttccgccggc acagcgcagc agagccggcc tgggtcgacg
     3541 ccgccgagct tcggcaacgg atcgcgtccg gcacgccgcc gctgatcctg gacgtgcgcc
     3601 agccggagga attcaccgcg ccgcccggcc acctgcccgg cgccctcaac ctgcctctgg
     3661 gccaggtcgc gcagcagacc gcccagcttt cggcgcggcg gcaagccgtc gttgtggtgt
     3721 gcaagaccga ccgccggtcg gctcgcgcgg cagccgaact gtgcgccgcg ggactgcagg
     3781 acgtatcggt gctgcgcggc ggcaccgatg gctggcatcg gctcgggctt ccgctggaat
     3841 gacgccggcc cgccgaaacg tcacgccgcg tcgcgcgtga gcaccccgcg gcggagctga
     3901 tcctcctcga tcgagacgaa cagcgcgcgg aaattgccct cgccgaaacc ctcgtcgccc
     3961 ttgcgctgga tgaactcgaa gaagatcggc cccaccagcg tcgccgagaa gatctgcagc
     4021 agcagcctgg tcttccgccc cgccgtttcg cccagcgcga ccgcgccctc gccgtccacc
     4081 aggatgccat tgcggcggag gcgctgcaga tcctcgccgt gcccgggcac gcgctcgtcc
     4141 acccgcgcat aataggtgtc gggaggtgac ggcatgaatt ccagcccatc gccgcgcagg
     4201 cgctcgacgg tggcgtggat gtcgcggcag ccacaggcga tatgctggat gccttcgccg
     4261 ttataggcgt gcaggaactc ctcgatctgg ctgcgcgcgt cggccgattc attgatggga
     4321 atgcggatct tgccgtccgg gctgctcagc gcgcgggaga acaggccggt gtattcgccc
     4381 ttgatgtcga aatagcggat ctcgcggaag ttgaagatgc gctcgtagaa cgcggcccag
     4441 tgggccatcc ggccggcgta gacgttgtgc gtcaggtggt cgatgatctc caggcaggcg
     4501 ccgacggggt gtttatcgac cccttcctcc caggtaaag
//
LOCUS       DASSZH010000223         3408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1198302, whole genome shotgun sequence.
ACCESSION   DASSZH010000223 DASSZH010000000
VERSION     DASSZH010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198302"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1989)
                     /locus_tag="VFN42_04795"
     CDS             complement(175..1989)
                     /locus_tag="VFN42_04795"
                     /inference="COORDINATES: protein motif:HMM:NF017730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HET8995965.1"
                     /translation="MEYAVNFGRLSRPARPASPGQKFRLALLGDFSGRANAGRLEIGE
                     ALAKRKPIKVDVDNLDDVLARMGLKLSLPMDEEGGTVEVEIGAIDDFHPDQLAENLDV
                     FGELRTLRRNQGNRASFDRAAKQVLSWGGEAPLPPPPRRARGTDVGHARLSDFARLIG
                     RPAAPEAEEASVDALMRRLVGPFVQPARDSRQDALVARVDEALSAAMCRVLHHPDFQT
                     AEALWRGVEFLVRRIETGAKMEIVLYDVSAEELAADLAASDTLDQTGLYGMLVEQPAL
                     DAHQGPLSVVAGFYGFELSPPHADLLGRIAQIAGAAGAPFIAGMAPDPLKLPFHDQHP
                     LIKDAFTALQALPAAAYLGLATPRFLLRMPYGRKTDPIDAFAFEEFTRQSGLGGMLWG
                     HPALIPALLLAESFAQQGTKLKLGAVMGVGDIPYYVYTGADGEQIALPCTERLYTERQ
                     AVQVSQYRVMPLLSIRGRPEVRLGGFMSQAGSPLAGFWAPVDIKPPAQKAAPAVAPAA
                     PAAKPEPAPAAAPEPEVEAAAEPAAAEAPAEDLDALLNSLQEPAAAPAESAAPAAPAE
                     EPAADDLDALLASLNAEPEPQASDETEPDLDALLASLK"
     gene            2086..>3408
                     /locus_tag="VFN42_04800"
     CDS             2086..>3408
                     /locus_tag="VFN42_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995966.1"
                     /translation="MKKGETPEFTVKWWKGSQPKGLKSAGGLESALKDYETAKGKLES
                     SGEDTDADAASKAMEDIIRAVKSVITEASKTKSDEMTATVDCLKKFDRLYAAERKWIE
                     EHTEASDDNAFGDPDVYHVYLLTMLKRLRSSGEMNFGFVLGKKAEDHRLALHKSKSSK
                     GLANLLVKQTGIHQMTWGIARPDEERGGLLVLTLEGRVLPGMAKKAGRMLKKFKPLPF
                     KKLALVVDGKEELEDLEAPDDGVDDDDLDTTVDTQQDARDATALTRELAELVRRVQGV
                     TDAGLKGDLGRMATQANALLRSNNLPEARQRIDEMRVALDRAPGNTQPGNGASGGAST
                     VAYAKSRLAWLAARKKVESEIDKLRSEIVATYKDDGIADQLESSFRTRVAPVLETLDE
                     SLADKLDEVANTTDATARAELVIEAKAIMQRYQSYLSSESLIADLDDNP"
BASE COUNT          595 a         1081 c         1230 g          502 t
ORIGIN      
        1 cgcctccagg cgctcgcgaa atgcgatggc cggatctttg acgacaaccc ccaggacacg
       61 ttccgtccag gcgttttgca ggtctgtgcc gctcatcgtg gatgggtaag cataggggtg
      121 ctttcgcttg caagtacttc cggcgtcagc gattcggccg cgccacggaa caggtcattt
      181 cagcgatgcc agcagggcat ccaggtcggg ctcggtctca tccgacgctt gcggctccgg
      241 ttccgcgttc aggctggcca gcagcgcgtc gaggtcgtcg gcggccggct cctcggccgg
      301 tgctgcgggt gctgcagatt ccgcgggtgc agcggcaggt tcctgcaggc tgttcagcag
      361 cgcgtcgaga tcttcggccg gcgcttcggc ggctgcaggt tcggcggcgg cttcgacctc
      421 cggctcgggc gcggcggcgg gtgcgggttc cggtttggct gcgggagcag cgggcgccac
      481 ggccggcgcg gccttctgtg ctggcggctt gatgtccacc ggcgcccaga acccagccag
      541 cggcgaaccc gcctgcgaca tgaagccgcc cagccgaacc tccggccggc cgcggatgga
      601 gagcagcggc atcacccggt actggctcac ctgcaccgcc tggcgctcgg tgtacagtcg
      661 ctcggtgcag ggcagggcga tctgctcgcc gtcggcaccg gtatagacgt agtacgggat
      721 gtcacccacg cccatcaccg cgcccagctt cagcttggtg ccctgctgcg cgaagctctc
      781 cgccagcagc agcgcgggga tcagcgccgg atggccccac agcatgccgc ccaggccgct
      841 ctgccgggtg aactcctcga aggcgaaggc gtcgatcggg tcggtcttgc ggccatacgg
      901 catgcgcagc aggaaacgtg gcgtggccag gccgagatag gccgcggccg gcaaggcctg
      961 cagcgcggtg aaggcgtcct tgatcaatgg atgctggtcg tggaacggca gtttcagcgg
     1021 gtccggtgcc atgccggcga tgaagggggc gcccgccgcg ccggcgatct gcgcgatgcg
     1081 gcccagcagg tcggcatgtg gcggcgacag ctcgaagccg tagaagccgg cgaccacgga
     1141 cagcggcccc tggtgcgcgt ccaatgccgg ctgctccacc agcatgccgt acagcccggt
     1201 ctggtccagc gtgtcggagg cggcgaggtc ggcggccagc tcctccgccg agacgtcgta
     1261 cagcacgatc tccatcttcg cgccggtctc gatgcggcgg accaggaact ccacgccgcg
     1321 ccacagcgcc tcggcggtct ggaagtccgg atggtgcagc acgcggcaca tggcggcgga
     1381 cagcgcctca tccacccggg cgaccagcgc atcctgccgg gaatcgcgtg ccggctggac
     1441 gaaggggccg accaggcggc gcatcagcgc atcgaccgag gcttcttccg cctccggcgc
     1501 ggcggggcgg ccgatcagcc gggcgaagtc ggacagccgc gcgtggccca catcggtgcc
     1561 gcgcgctcgc cttggtggcg gcggcagcgg tgcctcgccg ccccaggaca gcacctgctt
     1621 ggcggcgcgg tcgaagctgg cgcggttgcc ctggttgcgg cgcagggtgc ggagttcacc
     1681 gaaaacatcc aggttttccg ccaactggtc gggatggaag tcgtcgatcg caccgatctc
     1741 gacctcgacg gtgccgcctt cctcgtccat cggcaggctg agtttgagcc ccatgcgggc
     1801 gaggacgtca tccaggttat cgacgtccac cttgatgggt ttgcgttttg ccaaggcctc
     1861 gccgatttcc agccggccgg cattggcgcg gccggagaaa tccccaagca gggcgaggcg
     1921 gaatttctgt cccgggctgg cgggacgggc agggcggctc aggcggccga aattgacggc
     1981 gtattccatg ctggcactcc gcatcgggtt cgagataata ccagcacatc attccaggcc
     2041 aaccacggcg tgcgatacgc cggccagccc gagggggaag tcgccatgaa aaaaggtgag
     2101 acgccggaat tcaccgtgaa gtggtggaag ggcagccaac cgaaggggtt gaagagcgcc
     2161 ggcgggctgg aatccgcgct gaaggattac gagaccgcca agggcaagct ggaatccagc
     2221 ggcgaggata cggatgccga cgccgcctcc aaggcgatgg aggacatcat ccgggcggtg
     2281 aaatcggtga tcaccgaggc gtccaagacg aaatccgacg agatgaccgc cacggtggac
     2341 tgcctgaaga agttcgatcg cctctatgcc gcggagcgga agtggatcga ggagcacacc
     2401 gaagcgagcg atgacaacgc gttcggcgat cccgatgtct atcacgtcta cctgctcacc
     2461 atgctgaagc ggctgcgcag cagcggcgag atgaatttcg gcttcgtgct gggcaagaag
     2521 gccgaggacc atcggctggc cctgcacaag agcaagtcgt cgaaggggct cgccaacctc
     2581 ctggtgaagc agaccggcat ccatcagatg acctggggca tcgcgcggcc ggatgaagag
     2641 cgcggcggcc tgctggtgct gacgctggag ggccgcgtgc tgcccggcat ggccaagaag
     2701 gccggacgga tgctgaagaa attcaagccg ctgcccttca agaagctcgc cctggtggtg
     2761 gacggcaagg aggagctgga ggatctggaa gctccggatg atggtgtcga tgacgacgat
     2821 ctggatacga cggtcgatac ccagcaggac gcgcgcgacg ccacggcgct gacgcgtgaa
     2881 ctggcggagc tggtccggcg ggtgcagggg gtgaccgatg ccgggctgaa gggcgacctg
     2941 ggccgcatgg cgacgcaggc gaatgcgctg ctgcgcagca acaacctgcc ggaagccagg
     3001 cagcggatcg acgagatgcg cgtggcgttg gaccgggcgc cgggcaacac gcagccgggc
     3061 aacggcgcga gcggcggcgc cagcacggtg gcctacgcca agtcacgcct ggcctggctg
     3121 gcggcgcgga agaaggtgga aagcgagatc gacaagctgc gcagcgagat tgtggcgacc
     3181 tacaaggatg acggcatcgc cgaccagctg gagtcgagct tccgcacgcg cgtcgcgccg
     3241 gtgctggaga cgctggacga gagcctggcc gacaagctgg acgaggtcgc caacaccacc
     3301 gatgccacgg cgcgcgccga gctggtcatc gaggcgaagg cgatcatgca gcgctaccag
     3361 agttatctga gcagcgagtc gctgatcgcc gacctggacg acaatccc
//
LOCUS       DASSZH010000224         4363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1215401, whole genome shotgun sequence.
ACCESSION   DASSZH010000224 DASSZH010000000
VERSION     DASSZH010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215401"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            508..774
                     /locus_tag="VFN42_04805"
     CDS             508..774
                     /locus_tag="VFN42_04805"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HET8995967.1"
                     /translation="MPTIPHIEKHFRASAAVRDLVIGMSDGLTVPFALVAGLSGAVEA
                     GRRGDHRRPGGSRRRCLRHRPPGELTMRPSDPPIMVQVAFRQPR"
     gene            826..1125
                     /locus_tag="VFN42_04810"
     CDS             826..1125
                     /locus_tag="VFN42_04810"
                     /inference="COORDINATES: protein motif:HMM:NF025051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HET8995968.1"
                     /translation="MTRSPWRSFVATAALAATITATASGAFASQEREAGCRMQSGATA
                     LTDQEIRGKLTAQGFTEIRSIGREDGCVEAKGIDKEGKRFEVYLHPATGEIVSRK"
     gene            1139..1681
                     /locus_tag="VFN42_04815"
     CDS             1139..1681
                     /locus_tag="VFN42_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HET8995969.1"
                     /translation="METAVLAKPPSKTVQVWDPLLRISHWTLAAGFAVAFIAEEGDLV
                     HQVAGYIVAGAVAIRLAWGFVGPRHARFGDFVTGPRVLFAHLADMLRFRERRYLGHNP
                     AAGAMAITLLGLMLWLAVTGWMLTTNALYGLKWLETLHEGAANLALGLVFLHIAAAVY
                     ESLRQRESLPWAMVTGRKRV"
     gene            1681..1998
                     /locus_tag="VFN42_04820"
     CDS             1681..1998
                     /locus_tag="VFN42_04820"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HET8995970.1"
                     /translation="MRRLLLAVVLLAAALTQAMADDDQDAARHAREAGTAVPFEQILQ
                     RARREHPGRLLGVEVEHHGERLVYELRILAPGGHIEEWRYDAQDASPVPQGHAGREHE
                     ERH"
     gene            1998..2657
                     /locus_tag="VFN42_04825"
     CDS             1998..2657
                     /locus_tag="VFN42_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8995971.1"
                     /translation="MRLLVVEDEPTLAAQLQAALDKAGFAVDLAADGEDATTLGDSEP
                     YDAVVLDLGLPKLDGLAVLRHWRKRGVKAPVLILTARSDWHNKVEGLNAGADDYLGKP
                     FVMDELVARLRALIRRTKGFAGAEIACGPIVLDSSAGKVTVDGKPVGLTAQEFRTLSY
                     LMHRQGRIVSQRELGEHIYDLDDERESNTIEVFIRRIRRKLGVDAIQTVRGLGYRLEA
                     P"
     gene            2654..4123
                     /locus_tag="VFN42_04830"
     CDS             2654..4123
                     /locus_tag="VFN42_04830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8995972.1"
                     /translation="MTLLARLSLKARLLVGAVLWIGVTLLATGLVLTALFSRHVERRF
                     DAELATHLDQLLASLQETGGAVRLVRPMTDPRFQRPYAALYWQVDLPDGSALRSRSLW
                     DSRLALPNDTPADGEMHRHLLAGPDGQHLVAVERKVSLPGRSDVIRAAVAGDLGVVHA
                     ATSEFARTAAIALGTLALGLMLSVVVLVLGTLRPLTRLQRALAAVRLGEAQQVAGDFP
                     AEVQPLVNDLNLLLRHDAAVLARARTEAGNLAHELKTPLAIIANAMSGTDAATVQEQI
                     VRMRRRIDYALTRARAAASGNVLGAITLAGPRLQRLTQAMRTLYGPNGIAIELADQSD
                     APVQVDARDFDEIVGNLLDNACKWARSRVRLSASQHEGMLRLAIEDDGPGLPHAERAL
                     VFERGRKLDEATPGSGLGLSIVRDLLDAYAGSIRLEDTPLGGLCAVVLVPLAQPSVAG
                     RHARIVAGPAAAISAEQAACDPEDNHGKRRRSWFGALFD"
BASE COUNT          681 a         1474 c         1507 g          701 t
ORIGIN      
        1 acccgcaacg gatggatgtg gcgcggtccc tgaaagttgg cgacatcgta accgcagtga
       61 tcagcccgaa cattgcggac tggatcgaac atgcaaggac tccgacctgg agtggtggcg
      121 gtggttgacc ggacgttgca ctgacccggc gtcggtcagc atgctgtgag agttgctacc
      181 ggttatgcgt ttgctgtacg gctttgacgg tcgtggctga aagccgcgct aatagcgccg
      241 ccgaggagcg gttgcgcccg gcagatgctt cagcgaggcg tttctcgcag gcgaggaaat
      301 aggcgcactc cctccactgg catggggttc ggatcaacgt tgcttcggta catgaagtag
      361 ctcctccatt cagtctcccg gctgatctgg cgtggatggg cagccggccg caaaatcatc
      421 tggcgcttgc atcgctgcct gcgtttgacc tgcatcaatg tatgatggtg cgcgggccgt
      481 accctgtctg gccgtaggac gacggcaatg ccgaccattc cgcacatcga gaagcacttc
      541 cgcgcctccg ccgcggtgcg cgacctggtc atcggcatgt ccgacggcct cacggtgccc
      601 tttgccctcg tcgccggcct gtccggcgcg gtggaggcgg gccggcgtgg tgatcaccgc
      661 cggcctggcg gaagtcgccg ccgctgcctt cggcatcgcc cgcctggtga gctgaccatg
      721 cgacccagcg atccgcccat catggttcag gttgcgttca ggcagccacg ctagtcagcg
      781 ggcgtccgcc gcagggcgga tcgtcaattg aaggaacata cgtcaatgac ccgttctccc
      841 tggcgcagct tcgtggccac cgctgccctg gccgccacca tcaccgccac cgcgtccggc
      901 gccttcgcct cccaggagcg cgaagccgga tgccgcatgc aatccggcgc gacggcactc
      961 accgaccagg agatccgcgg caagctgacg gcgcagggct tcaccgagat ccgcagcatc
     1021 ggccgcgagg acggctgcgt cgaggcgaag ggcatcgaca aggaaggcaa gaggttcgag
     1081 gtctatctgc accccgccac cggcgagatc gtcagccgca agtagcagga gatcggtcat
     1141 ggaaaccgcg gttctggcga agccaccatc gaaaaccgtg caggtctggg atcctctgct
     1201 gcgcatctct cactggacgc tggccgccgg cttcgcggtg gcgttcatcg ccgaggaagg
     1261 cgacctggtg caccaggtcg ccggctacat cgtcgccggc gcggtggcga tccgcctggc
     1321 atggggcttc gtcggcccac ggcatgcgcg gttcggcgat ttcgtcaccg gcccgcgcgt
     1381 tttgttcgcg catctggcgg acatgctgcg gtttcgcgaa cgccgctacc tcggccacaa
     1441 ccccgccgcc ggcgccatgg ccatcaccct gctcgggttg atgctctggc tggcggtcac
     1501 cggctggatg ctgaccacca acgccttgta cggcctgaaa tggctggaga cgctgcacga
     1561 gggcgccgcc aacctggccc tcggcctggt gttcctgcac atcgccgccg ccgtgtacga
     1621 aagcctgcgg cagcgcgaga gcctgccctg ggcgatggtc accggccgaa agcgtgtgtg
     1681 atgcgacgcc tgctgctggc cgttgtcctg ctcgccgccg ccttgaccca ggcgatggcg
     1741 gatgacgacc aggacgcagc gcgccacgcc cgcgaagccg gcaccgcggt tcccttcgaa
     1801 cagatcctgc aacgcgcgcg gcgtgaacac ccggggcgcc tgctgggcgt cgaagtcgag
     1861 catcacggcg aacgcctggt ctatgagctg cgcatcctcg cacccggcgg tcatatcgag
     1921 gaatggcgct acgatgcgca ggacgccagc ccggtccccc agggacatgc cggccgtgag
     1981 catgaggagc ggcactgatg cggctcctgg tggtcgaaga cgaaccaacc ctggcggcgc
     2041 agctgcaagc ggcgctcgac aaggccggct tcgccgtcga tctcgccgcc gacggcgagg
     2101 acgcgaccac actcggcgac agcgagccgt atgacgcggt ggtgctcgac ctgggcctgc
     2161 cgaagctgga cgggctggcg gtgctgcgcc actggcgcaa gcgcggcgtg aaggcgccgg
     2221 tgctgatcct caccgcgcgg tcggactggc acaacaaggt ggaagggctg aacgccggcg
     2281 ccgacgacta cctcggcaag cccttcgtca tggacgagct ggtggcgcgg ctgcgcgccc
     2341 tgatccggcg caccaagggc ttcgccggcg cggaaatcgc ctgcggcccg atcgtgttgg
     2401 acagcagcgc cggcaaggtc accgtcgatg gaaagccggt ggggctgacc gcgcaggaat
     2461 tccgcaccct cagctacctg atgcaccgcc agggccgcat cgtctcccag cgcgaactcg
     2521 gcgagcacat ctacgacctg gacgacgagc gagaatccaa cacgatcgaa gtcttcatcc
     2581 gccgcatccg ccgcaagctc ggcgtcgatg ccatccagac cgtgcgcggc ctcggctacc
     2641 ggctggaggc gccatgaccc tgttggcccg gctgtcgctg aaggcccgcc tgctggtggg
     2701 ggcggtgctg tggatcggcg tcaccctgct ggccaccggg ctggtgctga ccgcgttgtt
     2761 cagccgccat gtcgaacgcc gcttcgacgc ggaactggcc acgcacctgg accagctgct
     2821 cgccagcctg caggagaccg gcggtgccgt gcgcctggtg cggccgatga ccgacccgcg
     2881 cttccagcgg ccctatgcgg cgctgtactg gcaggtcgat ctgccggacg gttcggccct
     2941 gcgctcgcgt tcgctctggg acagcagatt ggcgttgccg aacgacaccc ccgccgatgg
     3001 cgagatgcac cgccatctcc tggccgggcc ggacggccag cacctggtcg cggtggaacg
     3061 caaggtcagc ctgccgggcc gcagcgacgt gatccgcgcc gccgtcgccg gcgacctggg
     3121 cgtggtgcat gcggcgacca gcgaattcgc ccgcacggcg gcgatcgccc tcggcaccct
     3181 ggcgctcggc ctcatgctgt cggtggtggt cctggtgctg ggcacgctgc gtccgctgac
     3241 ccggctgcag cgggcgctcg ctgccgtgcg gctcggcgag gcacaacagg tcgccggcga
     3301 cttcccggcc gaggtgcagc cgctggtgaa cgacctgaac ctgctgctgc ggcatgacgc
     3361 cgcggtgctg gcacgcgcgc gcaccgaggc gggcaacctg gcgcatgaac tgaagacgcc
     3421 gctcgccatc atcgccaacg cgatgtccgg cacggacgcc gccacggtgc aggagcagat
     3481 cgtgcggatg cggcggcgga tcgactacgc gctgacgcgc gcccgtgcgg cggcgtccgg
     3541 caatgtgctg ggcgcgatca cgctggcagg cccgcgcctg caacgactaa cgcaggcgat
     3601 gcgaacgctg tatggcccca acggcatcgc gattgagctg gccgaccaga gcgatgcgcc
     3661 ggtccaggtt gatgcacgcg acttcgacga gatcgtcggt aacctgcttg acaatgcctg
     3721 caaatgggcg cggtcgcggg tccggctgag cgcgtcgcag cacgagggca tgctgcgcct
     3781 ggcgatcgag gatgacggtc ccggcctgcc gcacgccgaa cgcgcgctgg tgttcgagcg
     3841 cgggcgaaag ttggacgaag cgacgcccgg ttcggggttg ggcctgtcga tcgtgcgcga
     3901 cctgctggac gcctacgctg gcagcatccg gctggaggac acgccgttgg gcggcctgtg
     3961 cgcggtcgtg ctggtgccgc tggcgcagcc atccgttgcc ggccggcacg cgcgcatcgt
     4021 cgcagggccg gccgcggcaa tctccgcaga gcaagcagcg tgcgaccccg aagacaatca
     4081 cggcaagcgg cggcgttcct ggttcggcgc gttgttcgat tgacgccgcg atcttcaccg
     4141 atcggcgcgg cattcaggtc gcgttcagct tgtcctgcta ggaactgcat gccgcctgac
     4201 gacaggcggt tcgcatccgg agaggacaga atgaagagga tttccctgaa ggccgcgttg
     4261 agcgggctcg ccgttgccgc tgttgccgcc ggtggcttcg cctatgcggc ggatgctgtc
     4321 aatcggcgtt gaatattgac ccccgatcgg cgtccaaaat tga
//
LOCUS       DASSZH010000225         3693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1062016, whole genome shotgun sequence.
ACCESSION   DASSZH010000225 DASSZH010000000
VERSION     DASSZH010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062016"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VFN42_04835"
     CDS             <1..769
                     /locus_tag="VFN42_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995973.1"
                     /translation="SASSKDGPVTGLRVHELAESVRACVTAPMPALSPVLDATVERLW
                     QGACARMARGNAGRLFNGQVFSADQIGPRVITGHLTEFRRVVAQFEQPALFAELGLRP
                     LAVCGVLCCADGVVVGRRHVDAIYQAGLWQMPPAGSVDASAVAADGTVDLYHQLQLEL
                     QEELGLPPDSVGAPRPLCVAEHADTHVSDLGLLLCTKLQGRAVLAAHATAGNREYDPL
                     RVVAFADVPRLVAELGDMLVASAPPFLVRAGLLAGAR"
     gene            complement(787..1290)
                     /gene="nuoB"
                     /locus_tag="VFN42_04840"
     CDS             complement(787..1290)
                     /gene="nuoB"
                     /locus_tag="VFN42_04840"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HET8995974.1"
                     /translation="MLKTLLQGLRPPLTEPRPDPDPELAALAGTLDAVARRKLGRSLA
                     IREVDAGSCNGCELEIHALGNAFYDLERFGLRFVASPRHADVLLVTGPVTKNMQEALL
                     RTWCATPDPKWVVAAGDCAANGHVFAGSYAVAGGVSSTVPVDLVIRGCPPTPMQLLSG
                     LLTLMRR"
     gene            complement(1304..2782)
                     /locus_tag="VFN42_04845"
     CDS             complement(1304..2782)
                     /locus_tag="VFN42_04845"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HET8995975.1"
                     /translation="MTLDALLETGERVGGHQPWPCFVVDAATWQKAADLLAAGELTLL
                     SVWGETGRAHMALLRPDDPPVTVLSLDCLDGRFPSIGRVHPPAIRLERAMRDLFGLEP
                     IGLPDTRPWLDHGRWGSDPPADYAFLPVEGHSLHQIPVGPVHAGIIEPGHFRFTADGE
                     TVVRLEERLGYVHKGIDALLHGAPIPRALRIAGRASGDSTVAYAFAFSRAVEAALSIM
                     VPPRAVALRGVLAELERVANHLGDFGAICNDAAFSLMLAHCGVLRERVLRTAGACFGH
                     RLMMDRIVPGGVASDLDRSGETAIRGLLAELRRGFPPLVTLFDETASLQDRTVGTGVL
                     RPELARRFGAGGYVGRASGRDFDARRRPGYPPYDGLLFESPVLQEGDVNARALIRARE
                     IEQSLSMIEQLLDQMPGGAIRASVEDAPLDPGEGMALVEAFRGDVLVWLRVAGGCVER
                     CHLRDASWFQWPLLEAVIEGNIVADFPLCNKSFNCSYSGHDL"
     gene            complement(2788..>3693)
                     /locus_tag="VFN42_04850"
     CDS             complement(2788..>3693)
                     /locus_tag="VFN42_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HET8995976.1"
                     /translation="RPVVGEGQDAMVWTLLIEHASTFDPALLNVAFVFLFLGYGTKVG
                     LAPLHAWLPDAHAEGPTPISAVLSGLLLNVALYALLRYKILLAAAPAAIAPGPLMVTM
                     GLVSLIFAAFMLYRRRDIKRLFAYSSIEHMGIIVFAFGMAGPLGNFAGLLQMTMHSLT
                     KSAIFFAVGHVSQVKGTQRIAEIGGLTETHPVLGWGLVAGVAAIAGLPPFGVFTSEFL
                     VVSSTFARQPLLAVLLIFGLLVAFGALLLRLTSVAFGEPHGQSSRTKASHIPMFAHLA
                     LVLVAGIWLPPPLVAWFQHVAAMLG"
BASE COUNT          659 a         1256 c         1246 g          532 t
ORIGIN      
        1 ttccgcatcc agcaaggatg gtccggtgac cgggctgcgg gtgcatgaac tggcggagtc
       61 ggtccgagcc tgcgtgaccg ccccgatgcc ggcgctgtcg cctgtcctgg acgccaccgt
      121 ggagcggctg tggcaaggtg cctgcgcacg catggcccgc ggcaatgccg gccgattgtt
      181 caacggccag gtgttcagcg ccgaccagat cgggccacgg gtgatcaccg ggcacctgac
      241 cgagttccgc cgcgtcgtgg cacaattcga gcagccggcg ctgttcgccg agcttggact
      301 gcggccgctg gccgtgtgtg gggtgttgtg ctgtgccgac ggcgtggtgg tgggccggcg
      361 gcatgtggac gcgatctacc aggccgggct gtggcaaatg ccgcccgccg gcagcgtgga
      421 cgcctcggcc gtggcggcag acggtacggt ggacctgtac caccagttgc aactagaatt
      481 gcaggaggag cttgggctgc cgccggacag tgtcggcgcg ccgcgtccgc tctgcgtggc
      541 ggaacatgcc gatacccacg tctcggatct gggactgctg ctgtgcacga agctgcaggg
      601 acgcgcggtg ctggccgccc atgccaccgc cgggaaccgc gagtacgatc cgctgcgcgt
      661 ggtagcgttt gctgacgtgc cccgcctcgt cgcggagctg ggcgatatgc tggttgcttc
      721 cgcgccgccg ttcctggtgc gagcgggatt gctggccggc gctcggtaac cgggtcgctc
      781 ggtaggttat cgccgcatca gcgtcagcaa gccgctgagc aactgcatcg gcgttggcgg
      841 acagccgcgg atcaccaggt ccaccggcac cgtgctgctg acaccaccgg ccacagcata
      901 gctgccggcg aagacgtgac cgttcgctgc gcagtcgccc gctgccacca cccatttcgg
      961 atcgggtgtc gcgcaccacg tgcggagcag cgcctcctgc atgttcttcg tcaccgggcc
     1021 ggtcaccagc agcacgtcgg catgacgggg cgacgcgacg aaacgcaggc cgaagcgctc
     1081 caggtcatag aaggcgttgc ccagggcatg gatttccagc tcgcagccgt tgcaggatcc
     1141 ggcatcgacc tcgcggatcg ccaggctgcg gccgagcttg cggcgggcga cggcatccag
     1201 cgtgccggca agcgccgcga gttcgggatc cggatcgggg cgcggttcgg tgagcggcgg
     1261 gcgcaggccc tgcagcaggg ttttcaacat ggcgggatcg cggtcacagg tcgtgccctg
     1321 agtacgagca gttgaagctc ttgttgcaca gcgggaagtc ggcaacgatg ttgccctcga
     1381 tcaccgcttc cagcagcggc cactggaacc acgacgcatc acgcaggtgg cagcgctcga
     1441 cgcaaccacc ggccacgcgc agccacacca gtacgtcgcc ccggaacgcc tctaccagcg
     1501 ccatgccttc gcccgggtcc aacggcgcat cctccacgct ggcccgaatc gcgccgcccg
     1561 gcatctggtc cagtagctgt tcgatcatcg acaggctttg ctcgatctcg cgcgcacgga
     1621 tcaacgcccg cgcgttgaca tcgccctcct gcaacaccgg cgactcgaac agcagcccgt
     1681 cgtacggcgg atatcccggg cgccgccgcg catcgaagtc gcggcccgaa gcgcgtccca
     1741 catagccgcc ggcaccgaag cggcgtgcca gctccggacg caggacgcct gttccgactg
     1801 tgcggtcctg cagtgacgcg gtttcatcga acagcgtcac cagcggcggg aagccgcggc
     1861 gcagctcggc cagcaggccg cggattgccg tctcgccact gcggtcgaga tcgctggcaa
     1921 cgccgcccgg cacgatgcgg tccatcatca gccgatgacc aaaacacgcg ccggcggtgc
     1981 gcagcacgcg ttcccgcagc accccgcaat gcgccagcat caggctgaac gcggcatcat
     2041 tgcagatcgc gccgaaatcg ccgaggtggt tggcgacgcg ctccaactcc gccagcacgc
     2101 cgcgcaacgc cacggcgcgc ggcggcacca tgatgctcag cgccgcttcc accgcgcggg
     2161 agaacgcgaa ggcgtaagcc accgtggaat cgccggaggc gcggccggca atgcgcagcg
     2221 cgcgcgggat cggcgcgcca tgcagcagcg cgtcaatgcc cttgtgcaca tagccaagcc
     2281 gttcctccag ccgcaccacg gtttcgccgt cggcagtgaa gcggaaatga ccgggctcga
     2341 tgatgccggc gtgcacgggg ccgaccggaa tctggtgcag gctgtggcct tccaccggaa
     2401 gaaacgcgta gtcggcgggc ggatcgctgc cccaccgtcc atggtcgagc caggggcgcg
     2461 tatccggcaa cccaatcggc tccaacccga acaggtcgcg catcgcacgt tccagccgga
     2521 tcgctggcgg gtgcacgcgg ccaatggaag gaaaccggcc atccaggcaa tccaggctga
     2581 gcaccgtcac cggcggatca tccggacgca gcagcgccat atgcgcacgg ccggtttcac
     2641 cccaaacgct gagcagcgtg agttcgcccg ccgcgagcag gtctgccgcc ttttgccagg
     2701 tcgcggcatc cactacgaaa cagggccagg gctggtgccc acccacccgc tcgccggttt
     2761 caagcaatgc atcgagcgtc atcgccatca ccccagcatc gccgcgacgt gctggaacca
     2821 cgcgaccagc ggtggcggca gccagatgcc agcgaccagc accagcgcca gatgcgcgaa
     2881 catcggtatg tgcgatgctt tggttcggct gctctggcca tgcggctcgc cgaacgccac
     2941 ggaggtcaac cgcagcaaca gggcgccaaa cgcgaccagc aggccaaaaa tcagcagcac
     3001 tgcgagcagc ggctggcggg caaaggtgga gctgacgaca aggaactcgc tggtgaacac
     3061 gccgaagggc ggcagtccag cgatggccgc gacgccggcc actagccccc agccgagcac
     3121 cgggtgggtc tcggtgagtc cgccgatctc ggcgatacgc tgggtgcctt tcacctgcga
     3181 cacgtggccg acggcgaaga agatcgccga cttggtcagg ctgtgcatgg tcatctgcag
     3241 caggcctgcg aaattgccca gcggaccagc catgccgaag gcgaacacga tgatgcccat
     3301 gtgctcgatc gacgagtagg cgaacaggcg cttgatgtcg cggcggcggt acagcatgaa
     3361 ggccgcgaaa atgagcgaga ccagccccat cgtcaccatc agcggaccgg gcgcgatcgc
     3421 ggcgggcgcg gcggcgagca atatcttgta gcgcagcagc gcatagagcg cgacgttcag
     3481 cagcaggccc gacagcacgg ccgaaatcgg cgtcggacct tcggcatggg cgtcgggcag
     3541 ccaggcatgc agcggcgcga gaccaacctt cgttccgtat ccgagaaaca gaaacacgaa
     3601 agcaacgttg aggagagctg gatcgaaggt tgacgcatgc tcgatcagaa gtgtccagac
     3661 catggcatcc tggccttcgc ccaccacggg gcg
//
LOCUS       DASSZH010000226         5956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_584632, whole genome shotgun sequence.
ACCESSION   DASSZH010000226 DASSZH010000000
VERSION     DASSZH010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584632"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VFN42_04855"
     CDS             <1..307
                     /locus_tag="VFN42_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8995977.1"
                     /translation="TVSAANRLVDEAAETIAALVWPDVRAALRLEAEAMPRFRVVKEK
                     RATFAATAAQERRRPGPRIGLANLVLAGDWTATGLPATIEGAIRSGRRAAAALLGEA"
     gene            complement(380..1264)
                     /locus_tag="VFN42_04860"
     CDS             complement(380..1264)
                     /locus_tag="VFN42_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET8995978.1"
                     /translation="MRFTEIGQQLRAYRMESGLRAEEIAARLGVSRAALYRYEKGEVI
                     KLDTIKRLAELLKISPLSLLGIGVEYYNRPVGYFERIRQVEETADQILQLSGSICYLT
                     TSEAYDSALAQAFDEAADQAGPDRLAMRSMAEQVLGILASRKRMYNLRRPGIIAMVSL
                     SSVQRILEGGIAAGVPISERLRRLCRDVALTEVENMAGLMETEPMGLQFGLLMGGEPS
                     SAFKILRARDRVSLAINPFRSDTHPSAQAGVAMITGADEAITAHQRVAEASWREALKG
                     PAGALRLRQLISSVVRVA"
     gene            complement(1432..2319)
                     /locus_tag="VFN42_04865"
     CDS             complement(1432..2319)
                     /locus_tag="VFN42_04865"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HET8995979.1"
                     /translation="MKIGFIGLGIMGRPMSLNLHAAQHSLIVHDRFTMPEDVAAVAEV
                     VSDGKTVAANSEVVIIMVPDTPDVEDVLFRPGGVAEGLKPGTLVIDMSSISPIATKAF
                     AKKINEKGCDYLDAPVSGGEVGAQQATLTIMVGGPEKAFERAMPLFEKMGKNITHVGP
                     NNGDGQTCKVANQIIVALNIQAVSEALVFAARAGADPAKIRQALMGGFASSRILELHG
                     ERMLKGTFNPGFRIRLHQKDLNLALSAAKELNLSLPNTAIAQQMFDSCAAHGGADLDH
                     SSMILAIERLGNTSVREKK"
     gene            complement(2345..3310)
                     /locus_tag="VFN42_04870"
     CDS             complement(2345..3310)
                     /locus_tag="VFN42_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HET8995980.1"
                     /translation="MLTRRLLLAALPASVAVTGAQAAPDSFAAFLASLRQQARHAGIA
                     AATLDHALTGLTPNQAVLKREQHQPEFTMTWARYRNLLVTGKRIDEGVAAYRQSRTVF
                     AAVRQRYAVDPGIIAGIWGLESSYGTKMGDFRVVDALATLAWATSRRRFFSSELLAAL
                     KILQHGDTTAARMTGSYAGAMGQPQFMPTSYLRYAVDFTGSGRRDIWTSVPDVLASIG
                     NYLAQSGWRGGQSWGQPVALPAGFHGQSGRDHHRPLSAWAAMGLRPMARRWKAMGNAP
                     AALLLPDGPQGDAFLVYANFAAIRRYNPSDFYALAVGLLGDAIVA"
     gene            complement(3646..5181)
                     /locus_tag="VFN42_04875"
     CDS             complement(3646..5181)
                     /locus_tag="VFN42_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HET8995981.1"
                     /translation="MAQQHPLRSGVAMVTIGTMLATMMQALDTTIANVALPYMSGTFA
                     SSYDESTWVLTSYIVAAAIMTPPTGWLAARLGRRRLFLISVLGFTVASALCGLSQSLS
                     QAVIARVLQGMAGAALVPLSQAILLDSYPREKHGQAMAIWGMGIMLGPILGPTLGGWL
                     TQHWSWRWVYFINLPIGVLAALVIGAYVRETETGKAQRFDLFGFTSLSIAIACLQFVL
                     DRGQLQDWFASWQIIIAAALAVFSFYIFVVHIFTTDNPFIRPNLFLNRNFALGLLFIF
                     IVGLVLYGTMALFPPLLQDLLDFPVVTVGILLAPRGVGTMIAMLVVGRLTRRLPPRAL
                     MVFGFLVNAYSMWGMSGFSLQISAWDVFTWGVLQGFGLGFIFVPLTATAFATLAVERR
                     TEAAGIYSLIRNLGSSIGISIVTTLVTRNTQINHASLAAHLTPLDRTMTLTPLARMLG
                     IHSQRWLELLNQEITRQASMIGYIDDFKLMMILTAISVPLVLLMSSPPAGAEPDGGSV
                     VTE"
     gene            complement(5422..>5956)
                     /locus_tag="VFN42_04880"
     CDS             complement(5422..>5956)
                     /locus_tag="VFN42_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995982.1"
                     /translation="VRPARHVKALGAKPAGHASHHVLHAVRPHHAAHLLGKAARPRHW
                     VETVCRLVPGALLGGGLLLAPPPATRTAPLPQPAPMVQPLPAAWSVPLFLPPPAPTQP
                     EPLPPPDLSGTDPGHIGDVGGGGTGEHPVGPLPSDDSQKDGAAAVDEAPGAALLVIAM
                     ALLALARSGRRRIRAAD"
BASE COUNT          986 a         1933 c         2114 g          923 t
ORIGIN      
        1 gacggtcagc gcggcgaacc ggctggtgga cgaggcggcg gagacgatcg ctgcgctggt
       61 ctggccggat gtccgcgcgg cgctgcggct ggaggcggag gcgatgccgc ggttccgggt
      121 ggtaaaggaa aagcgcgcca ccttcgcggc gacggcggcg caggagcgcc gcaggccggg
      181 gccgcgcatt ggcctggcga atctggtgct ggccggcgat tggacggcaa ccggcctgcc
      241 ggcgaccatc gagggcgcga tccgctccgg ccgccgggcg gcagcggcgc tgctgggcga
      301 ggcgtgaagc acgccaaggc ggcaagacca tgccgccctt ttcaaaagcc tgggggtgcc
      361 gccggccgct ggcgcgcggc tacgccaccc ggaccacgct gctgatcaac tgccgcagcc
      421 ggagggcgcc ggccggaccc ttcagggcct cgcgccagct cgcctcggcg acgcgctggt
      481 gggcggtgat cgcctcatcg gcgccggtaa tcatcgccac cccggcctgc gcgctgggat
      541 gcgtatcgga gcggaacggg ttgatcgcca ggctgacgcg gtcgcgcgcc cgcaggatct
      601 tgaaggcgga actcggctcg ccgcccatca gcaggccgaa ctgcagcccc atcggctcgg
      661 tctccatcag cccggccatg ttctcaacct cggtcagcgc cacgtcgcgg cacagccggc
      721 gtagccgctc ggagatcggc accccggcgg cgatgccgcc ttccaggatg cgctgcacgg
      781 aggacagcga caccatggcg atgatgccgg gccgccgcag gttgtacatg cgtttgcggg
      841 aggccaggat gcccagcacc tgctcggcca tgctgcgcat tgccagccgg tcggggccgg
      901 cctggtcggc cgcctcgtcg aaggcctgcg ccagggcgct gtcatacgcc tcggaagttg
      961 tcagatagca gatggagccg gacagctgca ggatttggtc ggcggtctcc tcgacctgcc
     1021 ggatgcgctc gaaatagccc accggcctat tatagtattc aacgccgatc cccagcagcg
     1081 acaggggcga gatcttcagc aactcggcca gacgcttgat ggtatcgagc ttgatcacct
     1141 cgcctttttc atagcgatac aaagccgcgc gcgaaacgcc caggcgcgcg gcgatttctt
     1201 ccgcgcgcag gccggactcc atgcgatatg cgcgcagttg ctgcccaatc tcggtaaacc
     1261 gcatcgacat tcgccgtcct ccgacagctc tgccccgggt cgagctccaa tcaggccaac
     1321 tgcgaaagga gtctcagaat cgaggcggcc ccgccagttt atggcgggac cgtcctgaaa
     1381 ttcaacccct gtgtagcggg atgcgactcg ttgcggacag tccttacaga actatttctt
     1441 ctcgcgaacg gaggtgttgc ccagccgctc gatggccagg atcatggagg agtggtcaag
     1501 gtccgcgccg ccatgcgcgg cgcaggaatc gaacatctgt tgtgcaatgg cagtattggg
     1561 caaggaaagg ttgagttcct ttgctgccga caaggccagg ttgaggtctt tctggtgcag
     1621 ccggatgcgg aaccccggat tgaacgtgcc cttcagcatg cgctcgccat gcagttccag
     1681 gatgcgggag gaggcgaagc cgcccatcag cgcctggcgg atcttcgccg ggtcggctcc
     1741 cgcgcgggcg gcgaacacca gcgcttccga caccgcctgg atgttcagcg cgacgatgat
     1801 ctggttggcc accttgcagg tctggccgtc gccattgttc gggccgacat gggtgatgtt
     1861 cttgcccatc ttctcgaaca gcggcatggc gcgctcgaac gccttttcgg gaccgccgac
     1921 catgatggtc agcgttgcct gctgcgcgcc gacctcgccg ccggacaccg gcgcgtccag
     1981 gtagtcgcag cccttctcgt tgatcttttt cgcgaacgcc ttggtggcga tcgggctgat
     2041 cgaggacatg tcgatcacca gcgtgcccgg cttcagcccc tccgccacgc cgccgggacg
     2101 gaacagcacg tcctccacgt cgggggtatc gggcaccatg atgatcacca cctcggagtt
     2161 ggcggcgacg gtcttgccgt cggagaccac ttcggcaacc gcggcgacgt cctccggcat
     2221 ggtgaagcgg tcgtgcacga tcagcgagtg ctgcgccgca tgcaggttga gcgacatggg
     2281 gcggcccatg atgccgaggc cgatgaatcc gattttcatg cgtgagtctc cttaggttgc
     2341 gggatcaggc gacgatggca tcgcccagca ggccgaccgc gagggcgtag aagtccgacg
     2401 ggttgtagcg gcggatcgcc gcgaaattgg catagaccag gaaggcgtcg ccttgcggtc
     2461 cgtccggcag cagcagcgcc gccggggcat tgcccatcgc tttccaccgt cgcgccatcg
     2521 gacgcaagcc cattgccgcc catgcggaca gcgggcggtg gtggtcgcgg ccggactggc
     2581 cgtggaaccc ggccggcagc gccaccggct ggccccagct ctggccgccg cgccagccgg
     2641 attgcgccag gtagttgccg atggacgcca gcacgtccgg cacgctggtc cagatgtcgc
     2701 gccggccgct gccggtgaaa tcgaccgcgt agcgcaggta gctggtgggc atgaattgcg
     2761 gctggcccat cgcgccggca tagctgccgg tcatccgggc cgcggtggtg tcgccgtgct
     2821 gcaggatctt tagcgccgcc agcaactcgc tgctgaagaa gcggcggcgg ctggtggccc
     2881 aggccagcgt ggcgagtgcg tccaccacgc ggaaatcgcc catcttggta ccgtagctgg
     2941 attccaaccc ccagattccg gcgatgatgc cggggtccac cgcatagcgc tgccgcaccg
     3001 cggcaaacac cgtgcggctt tgccggtagg ccgccacccc ctcatcgatc cgtttgccgg
     3061 tcaccagcag gttgcggtac cgcgcccagg tcatggtgaa ctcgggctgg tgctgctcgc
     3121 gcttgagcac ggcctggttc ggcgtcagtc cggtgagagc atggtccagc gtggcggcgg
     3181 caatgccggc atggcgggcc tgttggcgca gcgaggcgag gaaggcggcg aagctgtccg
     3241 gggccgcctg cgcgccggtt acggcaacgg aggcgggcag ggcggcgagc agaaggcggc
     3301 gtgtgagcat ggcgcgactc ctgccacggc ccgccgtgtc ggagcaatcg gggaacgcga
     3361 ggcgcggttg ccaggacgac tggaggcggc gccgtcgcgg cttggcggct ggcgtattga
     3421 taagcaggag gcgcagagcg acagagttcg cagggctatg gataccagca tgcatgccgg
     3481 tggatcgcag gcacgtaaat cgtgtcggtg agttctgcag ctctgtgcct catgcttgtg
     3541 aatacgcaat aagcgtctgc cttccgcatc gcggatccgc tcccgcaagc cggcggccac
     3601 cctgagctgc ggtcttgctg ggtccgtgac gcggtgggtg ggtcattatt ccgtcaccac
     3661 cgatccgccg tcaggttccg cgcctgccgg cggcgacgac atcagcagca ccagcggcac
     3721 cgagatcgcg gtcaggatca tcatcagctt gaagtcgtcg atatagccga tcatcgacgc
     3781 ctgccgcgtg atctcctggt tcagcaactc cagccagcgc tgcgaatgga tgcccagcat
     3841 gcgggccagc ggcgtcagcg tcatcgtgcg gtccagcggc gtcaggtgcg cggcgaggct
     3901 cgcgtgattg atctgcgtat tgcgcgtcac cagcgtggtg acgatggaaa tgccgatgct
     3961 gctgccgagg ttgcggatca ggctgtagat gccggctgcc tcggtgcgcc gctccaccgc
     4021 cagggtggcg aaggcggtgg cggtcagcgg cacgaagatg aagcccaggc cgaagccctg
     4081 cagcacgccc caggtgaaca cgtcccaggc gctgatctgc agcgagaagc cggacatgcc
     4141 ccacatcgaa taggcgttca ccaggaaccc gaacaccatc agcgcccgcg gcggcaggcg
     4201 gcgggtcagc cgccccacga ccagcatggc gatcatcgtg ccgacgccgc gcggcgccag
     4261 caggatgccg accgtcacca ccgggaaatc cagcaggtcc tgcagcagcg gcgggaacag
     4321 cgccatcgtg ccgtacagca ccaggccgac gatgaagatg aacagcagcc ccagggcgaa
     4381 attgcggttc aggaacaggt tgggccggat gaacggattg tccgtggtga agatgtgcac
     4441 cacgaaaatg tagaaggaga acaccgcgag ggcggcggcg atgatgatct gccaggaggc
     4501 gaaccaatcc tgcaactggc cgcggtccag cacgaattgc aggcaggcga tcgcgatgct
     4561 gaggctggtg aagccgaaca ggtcgaaccg ctgcgccttg ccggtttcgg tctcgcgcac
     4621 ataggcgccg atcaccagcg cggcgaggac accgatcggc aggttgatga aatacaccca
     4681 gcgccagctc cagtgctggg tgagccagcc gcccagcgtc ggtcccagga tcgggccgag
     4741 catgatgccc atgccccaga tcgccatggc ctggccgtgc ttctcgcgcg gatagctgtc
     4801 cagcaggatc gcctgcgaca gcggtaccag cgcggcccct gccatgcctt gcaggacgcg
     4861 ggcgatcacc gcctgcgaca gcgactgcga caggccgcac agcgcggagg ccacggtgaa
     4921 gccgaggacg gagatcagga acagccgccg ccggcctagc cgggcggcca gccaaccggt
     4981 gggcggcgtc atgatcgccg cggcaacgat gtaggaggtg agcacccagg tgctctcgtc
     5041 gtagctgctg gcgaaggtgc cggacatgta gggcagggcg acgttggcga tggtggtgtc
     5101 cagcgcctgc atcatggtgg cgagcatggt gccgatggtg accatcgcca cgccgctgcg
     5161 aagtgggtgc tgctgcgcca tgcccccgtg gataacctcc tgaacgatcc ctgatgccga
     5221 gataggcgca tgtggcgcgc gtgggaaccg ggtctggcgc caccgtggca tgatgctgcg
     5281 gcggtgcaaa tccggcgcga ttgccgtttg ctgaatccgg cgtgccgccg ctgctgcggg
     5341 catggccgaa gcagggcagc acggagggca cgcagtaccg ggggcatggc tgcgcgacca
     5401 ccgcgcaagg ctgcgtctgg ttcaatcggc cgctcggatg cggcgccgcc cggagcgtgc
     5461 cagggcaagc agcgccatcg cgatgaccaa caacgctgcg cccggagctt catccacggc
     5521 ggcggcacca tccttctgcg aatcatcgct cggcagcggc ccgaccggat gctcgccggt
     5581 tccgccgccg ccgacatcgc cgatgtggcc tgggtccgtt ccggagaggt cgggcggcgg
     5641 gagtggctcg ggctgcgtcg gtgcgggcgg tgggaggaac aacggaacgg accaggcggc
     5701 tggcagcggc tgcaccatgg gtgcgggctg cggcaggggc gccgtcctgg tggccggcgg
     5761 cggcgccagc agcaggccac cgcccagcag agcgcccggc acgaggcggc agacggtctc
     5821 gacccagtgg cgcgggcgtg ccgccttgcc gaggaggtgg gcggcatgat gcggacgcac
     5881 cgcgtgcagg acatggtgcg acgcgtgccc ggccggcttg gcacccaatg ccttgacgtg
     5941 gcgggccggc ctgacg
//
LOCUS       DASSZH010000227         3352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_806272, whole genome shotgun sequence.
ACCESSION   DASSZH010000227 DASSZH010000000
VERSION     DASSZH010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806272"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="VFN42_04885"
     CDS             <1..1000
                     /locus_tag="VFN42_04885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I secretion C-terminal target
                     domain-containing protein"
                     /protein_id="HET8995983.1"
                     /translation="PATLSAAAGQALAVSGVSLSDPWAANHPGSLALNVTASSGTVSM
                     KDANGNPLGGSGSGAIHTSGSLAQITAELAGLSYTPGSNGGSVTIDVWDQAGAEATKS
                     FAVTISGTGGTDPTGGTGGTTSTTGGTTGTTGGTTGGTGSTPSNLPTVTIAANDASPV
                     ESVSNTQITASSGDHMIFINGTGDVLNATGGTETVMAFQGGNQITTGAGNDLIRFAGN
                     GNVVNAGAGQNVLQDSGSNGTIVLPGANQGFDNIYGWVLQNGDKLDLRQALAGTSWQQ
                     GDLASIGNYVKVAASGDGTSISIDPTGTPGGASYAIATLQASGPSTLSTVLAHSIT"
     gene            1449..2690
                     /locus_tag="VFN42_04890"
     CDS             1449..2690
                     /locus_tag="VFN42_04890"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET8995984.1"
                     /translation="MTSSTFPGLGLSEPLLRALVEQQFHTPTPIQAGAIPALLAGRDL
                     LGVAQTGTGKTAAFGLPLLQALAADNVRAAPFGTRALILAPTRELAQQIDDSLRRFAA
                     NLRLRSVTILGGVSRFRQVQQMRRGTDIVVGTPGRVCDLMATGELRLGSVIQFVLDEA
                     DRMLDLGFIRDIRRIEAALPAQRQSALFSATMPKEVAGLAESLLRDPVRVSIAPVAAT
                     TPKIEQCVHFVRQPDKRPLLIDLLGDPGMQRVIVFTRTKHGADRVAEGLEQAGQSVSA
                     IHGNKSQPQRERALDRFRNGRARVLVATDIAARGIDVTGISHVVNYDIPVEPESYVHR
                     IGRTARAGASGVAISFCDPAEHGALRAIERCTGAAIPVAGGTPPEAPAKRSGKPADKS
                     AGKPARSRRQRRHRGGMRAAA"
     gene            2687..3151
                     /locus_tag="VFN42_04895"
     CDS             2687..3151
                     /locus_tag="VFN42_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HET8995985.1"
                     /translation="MTALYVDGDACPVRDEVYRVADRVGLAVFVVSNASRPIRPTGRS
                     NVHLVTVPATPDAADDWIADRITPADICITADIPLAARCLEKGAQAVAPAGKVWTTDN
                     IGSALAGRELSRHLREIGLNSGGPPPLTRQDRAQFLNALDAAVRKAAGRNAG"
BASE COUNT          528 a         1243 c         1103 g          478 t
ORIGIN      
        1 gccggccacg ctgagcgccg cggccggcca ggccctggca gtcagtgggg tgtcactcag
       61 cgacccgtgg gcggccaatc atccaggctc gctggcgctg aacgtcacgg ccagcagcgg
      121 caccgtcagc atgaaggatg ccaatggcaa tccgctgggc ggttccggct ccggcgccat
      181 ccatacctcg ggcagcctgg cgcagatcac cgccgagctg gcagggctga gctatacgcc
      241 cggcagcaat ggcggttcgg taacgatcga cgtgtgggac caggccggcg cggaggcgac
      301 gaaatccttc gcggtgacca tctccgggac cggcggcacc gatccgacgg gtggcaccgg
      361 cggcacgacc tccaccacgg gtggcaccac cggcacgacg ggtggcacca ccggcggcac
      421 gggctcgacc cccagcaacc tgcccaccgt caccatcgcg gcgaacgacg ccagcccggt
      481 ggagagcgtc agcaacacgc agatcaccgc cagctccggt gaccacatga tcttcatcaa
      541 cggaaccggc gacgtgctca acgccaccgg cggcaccgag acggtcatgg cgttccaggg
      601 cggcaaccag atcaccaccg gtgccggcaa cgacctgatc cgcttcgccg gcaacggcaa
      661 cgtggtcaac gccggtgctg gccagaacgt gttgcaggac agcggcagca acgggacgat
      721 cgtgttgccc ggcgccaacc agggcttcga caacatctac ggctgggtgc tgcagaacgg
      781 cgacaagctg gacctgcgcc aggcgctggc gggcaccagt tggcagcagg gcgacctggc
      841 cagcatcggc aactacgtca aggttgccgc cagcggcgat ggtacgagca tcagcatcga
      901 tccgaccggc acccccggcg gcgcgagcta cgcgattgcg acgctgcagg cgtccggccc
      961 gagcacgctc agcacagtgc tggcgcattc gatcacctga gttggctgtg ggcccctctt
     1021 cgccgccagg cggagagggg gctgcagcgc cgccgcctca gaagggcacg cgcctggcca
     1081 ctgccgcggt caccatcgcc gccacaggcc cgccctgact tcacgccacg cctttgccgc
     1141 aaggaacgct cggggccggg ttcggccgcc cccgccgttc cgattcgcgt gtactgcggc
     1201 gggggaaggc ccacgcaaca agcccccggc aaactgcgca gcgccagatc gcgcttgcct
     1261 ttcgttcaac accggcccac ataggttgcg gcttccgtga ctgcgtgatg cgtgcgcccc
     1321 tggcgccgcc ctcccggaaa atccattcgc aacgcgttcg ccccatcatc gcgcgggcag
     1381 gcggacattc aggcgctcgc ctccatcccg gactctgtcc gaccggcggc gctggagctt
     1441 ctatccacgt gacatcttcg acgttcccgg gcctcggcct gtccgagccc ttgctgcgcg
     1501 cgcttgtcga gcagcagttt catacaccga cgccgatcca ggccggcgcc attccagcct
     1561 tgctcgccgg ccgtgacctg ctgggcgtcg cccagaccgg caccggcaaa accgccgctt
     1621 tcggcctgcc gctgctgcaa gccctggccg ccgacaacgt ccgcgccgcc ccgttcggca
     1681 cccgcgccct gatcctggcc cccacccgcg aactggcgca gcagatcgac gacagcctgc
     1741 gccgcttcgc cgccaacctg cggctgcgca gcgtcaccat cctcggcggc gtcagccgct
     1801 tccggcaggt gcagcagatg cgccgcggca ccgatatcgt cgtcggcacc cccggccgcg
     1861 tctgcgacct gatggccacc ggcgaactgc ggctcggcag cgttattcag ttcgtgctgg
     1921 atgaggcgga tcgcatgctg gatctgggct tcatccgcga catccgccgg attgaagcgg
     1981 ccctgccggc gcagcgccag tccgcgttgt tttccgccac catgccgaag gaagtcgccg
     2041 gtctggccga gtcgctgctg cgcgacccgg tgcgcgtctc gatcgccccg gtcgccgcga
     2101 ccacgccgaa gatcgaacaa tgcgtgcatt tcgtcaggca gccggacaag cgcccgctgc
     2161 tgatcgatct gctcggcgac ccgggcatgc agcgggtgat cgtgttcacc cgcaccaagc
     2221 acggcgccga ccgcgtggcg gaagggctgg aacaggccgg ccaatcggtc agtgcgatcc
     2281 acggcaacaa gagccagccg cagcgcgagc gggcgctgga ccggttccgc aacgggcgcg
     2341 cccgcgtcct ggtggccacc gacatcgctg cgcgcggcat cgacgtcacg gggatatccc
     2401 acgtggtcaa ctacgacatt ccggtagagc ccgagagcta cgtgcaccgc atcggccgca
     2461 cggcgcgcgc cggtgcttcc ggtgtggcga tctccttctg cgacccggcc gagcatggcg
     2521 cgctacgggc gatcgagcgc tgcaccggcg cggccatccc ggtcgccggc ggcaccccgc
     2581 cggaggcgcc ggcaaagcgc tccggcaagc cggcggacaa gtcggcgggc aagccggccc
     2641 gctcgcgccg ccagcggcgg caccgtggcg gcatgcgcgc cgcggcatga ccgcactgta
     2701 cgtcgatggc gatgcctgcc cggtgcgcga cgaggtctat cgcgttgccg accgggtcgg
     2761 ccttgccgtc ttcgtcgtgt ccaacgcctc ccgcccgatc cggccgaccg ggcggagcaa
     2821 cgtgcacctg gtcaccgtgc ccgccacgcc ggatgccgcg gatgactgga tcgcggaccg
     2881 tatcaccccg gcggatatct gcatcaccgc cgatattccg ctggcggcac gctgcctgga
     2941 gaagggtgcg caggcggtgg cccccgccgg caaggtctgg accaccgaca atatcggcag
     3001 cgcgctcgcc gggcgcgagt tgtcgcgcca tctgcgggag atcgggctga atagcggcgg
     3061 cccgccgccg ctgacccgcc aggatcgcgc gcagttcctg aatgcgctgg atgccgccgt
     3121 gcgcaaggcg gcggggcgca acgccggctg acccggcaat cgtcataccc ggggcaagcc
     3181 acgtgcccag ccctcgcggc ggctccgcct gcgcagtttt tgcacccccc gtttacgctt
     3241 tctttgccct tccaggctca tggtcaccgg accgacaggt cagccaaagg tgcacccatg
     3301 gcaaaagcgt tcctcaaaat cctgctcctg cttggcatgg tgttcagtgc ga
//
LOCUS       DASSZH010000228         4207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1198446, whole genome shotgun sequence.
ACCESSION   DASSZH010000228 DASSZH010000000
VERSION     DASSZH010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198446"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VFN42_04900"
     CDS             complement(<1..386)
                     /locus_tag="VFN42_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995986.1"
                     /translation="MNGPVLTLFAALIAALFVLGGFGLAWPRPMRPVAGLIAGLAGGV
                     GAMLAMAVLLLPGPPAELVLPVGLPGLPLVLAVDSLSGGFLLLVSLAGTAAIVFGAEI
                     ASAARPDLTPTLAIALAGLLLAVLAA"
     gene            483..1265
                     /locus_tag="VFN42_04905"
     CDS             483..1265
                     /locus_tag="VFN42_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8995987.1"
                     /translation="MHEAIPSSIPRVALITGGAQRIGRAIGLALAGAGFDVAVHCNAS
                     RAACEDTCAAIRDRGRRAVVLQADLSDEAQSNELLPAAAAALGPVGVLVNNASLFERD
                     EWHDASRASWDAHLEPNLRAPFVLTQRFAQALPETAEGVAINLLDQRVWSLTPHFVSY
                     TLSKAGLWALTQSMALALAPRIRVNGIGPGPALPSPRQSQEQFDRQAESVPLRRPTSP
                     EEIGRAALAILALPSMTGQMLALDGGQHLQWASGRNAAPPEE"
     gene            1269..1697
                     /locus_tag="VFN42_04910"
     CDS             1269..1697
                     /locus_tag="VFN42_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HET8995988.1"
                     /translation="MESHPQFASSARALRHVFLRDLVLSASIGVHPHEHAARQRVRIN
                     VDLAVEDDSARGLSRGGVGRDELSRVVDYEKVADAIRAIVTSGHVRLVETLAERIAES
                     CLSDERVHLARVRVEKLDIFADATSAGVEIERRRGDLSTR"
     gene            2080..2409
                     /locus_tag="VFN42_04915"
     CDS             2080..2409
                     /locus_tag="VFN42_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HET8995989.1"
                     /translation="MSDEPGGGGPSFEERLKAARRRRGLEPQPKPDGPGSDDFGPSPL
                     SIGLRVGVELVSALAVAFAIGYGLDRWFGTKPVFIIIFVLLGGAAGVLNVWRMFAPRD
                     GSDRGYK"
     gene            2441..3166
                     /locus_tag="VFN42_04920"
     CDS             2441..3166
                     /locus_tag="VFN42_04920"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET8995990.1"
                     /translation="MSQFQLHPALGIVGSSVNFTNSNLFMVIAGALMLALLWLGMRPR
                     AMVPGRFQSAAEMAYEAVMSMCVDTIGPEGKAYFPFVFTLFFFILFGNLLGVWPYDFT
                     FTSHIVVTLALTMVVVILTTFVGLRIHKLHWFSYFVPEGVPKLLLPLLVPIEVLSYLS
                     RIISLSVRLFANMMAGHVMLEVFGAFVVMLGTAGAIFWIPSALTLGVNVALIGFEILV
                     GTLQAYVFAILTCIYLHDAVHLH"
     gene            3237..3461
                     /locus_tag="VFN42_04925"
     CDS             3237..3461
                     /locus_tag="VFN42_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C family protein"
                     /protein_id="HET8995991.1"
                     /translation="MDLASAKEIGAGLAVIALSGVGVGIGYIFAALVSSVARNPASRD
                     RVFGLAILGFALTEAVALYALVIAFIILFV"
     gene            3470..4030
                     /locus_tag="VFN42_04930"
     CDS             3470..4030
                     /locus_tag="VFN42_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B'"
                     /protein_id="HET8995992.1"
                     /translation="MRLLFAAFLLLPTAAMAEGMPQLDFANPLTTSQVVWGAIIFALL
                     YVLAKQFALPKVGDVLEERATHIARDLESAQAAKNKADAAVDELTQATAKARAEAQAA
                     INAALEQAKAEAARQAAELNERLERQLAESEGQIAAARAAAMGALRQVATDTASTMVV
                     RLTGTAANDDRLHSAIDRALSARGVG"
     gene            4045..>4207
                     /locus_tag="VFN42_04935"
     CDS             4045..>4207
                     /locus_tag="VFN42_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HET8995993.1"
                     /translation="MEGGSFLTEGRSWVAIAFVIFFLVFGKKLWGALAQMLDDRAEKV
                     RAELDEASRL"
BASE COUNT          689 a         1349 c         1448 g          721 t
ORIGIN      
        1 tccgcggcca gcacggcgag cagcaggccg gccaacgcga tggccagggt cggtgtcaga
       61 tccgggcgcg cggcggaggc tatctcggcg ccgaacacga tcgcggcggt gccggcgaga
      121 gagaccagca gcaggaagcc acctgacagc gagtcgaccg ccagcaccag cggcagcccc
      181 ggcagcccga ccggtagcac cagctcggcc ggcggccctg gcagcagcag cacggccatg
      241 gcgagcatgg cgccgactcc gccggccagg ccggcgatca ggcccgcgac cggccgcatc
      301 gggcgcggcc aagccaagcc aaacccgcca agcacgaaca gcgcggcgat caaggccgcg
      361 aagagggtga gaaccggacc gttcacccgg aaaggctagc agacggtgcc gagtcgtggc
      421 cggccttctg tcaccgggcc gggtgtggca cgttgaatgc gtcatcacag ccgacggtat
      481 ccatgcacga agccatcccc tccagcatcc cgcgcgttgc cctgatcacc ggtggggcgc
      541 agcggatcgg ccgagccatc gggttggcgt tggccggggc gggattcgat gtcgcggtgc
      601 actgcaacgc cagccgcgcc gcctgtgagg acacctgtgc ggccattcgt gaccgcggcc
      661 ggcgtgccgt ggtgctgcaa gccgacctgt cggacgaagc gcagagcaat gagctgctgc
      721 cggccgccgc cgccgctctg gggccggtcg gcgtgttggt gaacaacgcc agcctgttcg
      781 agcgggacga atggcacgac gcctcgcgcg cgtcctggga cgcgcatctg gaacccaatc
      841 tgcgtgcgcc cttcgtgctg acccagcgct tcgcccaggc gctgccggaa acggcggaag
      901 gcgtggcgat caatctgctg gaccagcgcg tctggtcgct gaccccgcat ttcgtgtcgt
      961 acaccttgtc caaggccggg ctttgggcgc tgacccagag catggcgctg gcgctggcgc
     1021 cgcgcatccg ggtgaacggg atcggccccg gtcctgccct gcccagcccg cggcaatcgc
     1081 aggaacaatt cgaccggcag gccgagtcgg tgccgctgcg gcgcccgacc agcccggagg
     1141 agatcggccg tgccgccctg gcgatcctgg ctctgccgtc gatgaccggg cagatgctgg
     1201 cgctggatgg tgggcagcat ttgcaatggg cgtcaggccg caacgccgcg ccgccggagg
     1261 agtaagcgat ggaatcccac ccgcaattcg ccagtagcgc acgcgctttg cgccatgtct
     1321 ttctgcgtga cctggtgctg tccgccagca tcggcgtgca tccgcatgaa cacgcggcgc
     1381 gccagcgggt gcggatcaac gtggaccttg cggtggagga cgacagcgcg cgcggcctgt
     1441 cgcgcggcgg cgtaggacgg gacgaattgt ctcgcgtggt ggattacgag aaagtggcgg
     1501 atgcgatccg cgcgatcgtc acttccggcc atgtccggct ggtggagacc ctggcggagc
     1561 gcatcgccga atcatgcctg tccgatgaac gggtccacct ggcccgggtg cgggtggaaa
     1621 agctggacat cttcgcagac gcaacatctg ccggggtgga gatagagcgg cggcggggcg
     1681 atttgtccac ccgctgaggc ggcgcgccga aaggtttgaa aaccggagga aaattgcagt
     1741 tgcgttacgg ggaaaaatat caatttaacg ccgaatcaat gggttagacg atgctcgaat
     1801 tttgggcaat ctttacggaa gccgctgaaa ccgcgggttt cacgaaatcg cgatagccaa
     1861 tgcccacaac tttatccaca ggatcggtgg atgcctcaca agcttcgccg gtccgactgc
     1921 ctatcgaaca accggcctga gggaacgcgg ctgaacttag cacggggagg gcgtcgtggg
     1981 gcatgcctgg gcgattgcaa gtctctggca ttcttgacac cccggtaacc cgcctgtagc
     2041 tagcgcgcgc cttccggcaa gccattgcca ggagcgaaaa tgagcgatga accgggtggc
     2101 ggtggcccct ccttcgagga gcggttgaaa gctgcccgac gcaggcgggg gctggagccc
     2161 cagccgaaac cagacgggcc ggggtccgac gatttcggcc cttcaccgct gagtatcggc
     2221 ctgcgggtgg gggttgaact ggtgtctgcc ctggcggtgg cttttgccat cggctacggg
     2281 ttggatcgtt ggttcggcac gaagccggtc ttcatcatca tcttcgtcct gcttggcggc
     2341 gcggcgggcg tgctgaatgt gtggcgcatg ttcgcgccgc gggacggatc ggacagggga
     2401 tacaagtagc gtggcggcgc aaacgggtac catcgacgcc atgagccagt tccagttgca
     2461 tccggcactg ggaatcgttg gctcgtcggt gaatttcacc aattccaacc tgttcatggt
     2521 gatcgccggc gcgctgatgc tggcgctgct gtggttgggc atgcggccgc gcgcgatggt
     2581 gccgggccgg ttccagtccg ctgcggaaat ggcctatgaa gcggtcatgt cgatgtgcgt
     2641 cgacacgatc ggccccgaag gcaaagcgta tttccccttc gtcttcacac tgttcttctt
     2701 cattctgttc ggcaacctgc tgggcgtctg gccgtacgac tttaccttca ccagccacat
     2761 cgtggtgacg ctggcgttga ccatggtggt ggtcatcctc accaccttcg tcggcctgcg
     2821 catccacaag ctgcattggt tcagctactt cgtgccggag ggcgtgccca agctgctgct
     2881 gccgctgctg gtgccgatcg aggtgctgtc ctacctgtcg cgcatcatct cgctgtcggt
     2941 tcgtctgttc gccaacatga tggcgggcca cgtcatgctg gaagtgttcg gcgccttcgt
     3001 ggtgatgctg ggcacggccg gcgcgatctt ctggatcccc tccgcgctga ccctgggggt
     3061 caacgtcgcc ctgatcggct tcgaaatcct ggtcggcacg ctgcaggcct atgtgttcgc
     3121 catcctgacc tgcatctacc tgcacgacgc cgtccacctg cactgaccct tccacccttc
     3181 ggtccggtca gcgcgtggct gccggatacg ccagtctcaa cgaaaggaac ttcccaatgg
     3241 atctcgcttc cgccaaggaa atcggcgcgg gcctcgccgt tatcgcgctg tccggcgtcg
     3301 gcgtcggcat cggctacatc ttcgccgcgc tggtctccag cgtcgcgcgc aacccggcct
     3361 cccgcgaccg cgtgttcggt ctggccattc tgggcttcgc gctgacggaa gcggtggcgc
     3421 tgtacgcgct ggttatcgcc ttcatcatcc tgttcgtctg agatccgcca tgcggctgct
     3481 gttcgcggcc ttcctgctgc tgcccaccgc cgcgatggcg gagggcatgc cgcagctgga
     3541 cttcgccaac ccgctgacca cgagccaggt ggtatggggc gcgatcatct tcgcgttgct
     3601 ctatgtcctg gccaagcaat tcgcgctgcc gaaagtgggc gacgtgttgg aggagcgggc
     3661 gacgcacatc gcgcgcgacc tggaaagcgc ccaggcggcg aagaacaagg ccgacgcggc
     3721 cgtggacgaa ctcacccagg ccaccgccaa ggcgcgtgcc gaggcgcagg ccgcgatcaa
     3781 cgcggcgctg gaacaggcca aggccgaggc cgcgcgccag gccgcggagc tgaacgagcg
     3841 gctggaacgg cagttggccg aatccgaagg ccagatcgcc gccgcccgcg ccgcggcgat
     3901 gggcgcgctg cgccaggtgg cgaccgatac cgccagtacc atggtggtgc ggttgaccgg
     3961 caccgcagcg aatgacgacc ggctgcacag cgcgatcgac cgggcgctgt cagcgcgcgg
     4021 cgtcggctga gagggagcgg agcaatggaa ggcggaagct ttctcaccga agggcgtagc
     4081 tgggtcgcga tcgccttcgt gatcttcttc ctggtgttcg ggaagaagct gtggggcgct
     4141 ctcgcgcaga tgctggatga ccgcgccgag aaggtgcggg ccgagctgga cgaagcctcg
     4201 cggctgc
//
LOCUS       DASSZH010000229         2474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_994045, whole genome shotgun sequence.
ACCESSION   DASSZH010000229 DASSZH010000000
VERSION     DASSZH010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994045"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFN42_04940"
     CDS             <1..219
                     /locus_tag="VFN42_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HET8995994.1"
                     /translation="EELDRLHSHVQAAHALLREAANIGRRFDFLVQEFNREANTLCSK
                     SASTALTATGLKLKATIEQLREQVQNIE"
     gene            216..854
                     /gene="gmk"
                     /locus_tag="VFN42_04945"
     CDS             216..854
                     /gene="gmk"
                     /locus_tag="VFN42_04945"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET8995995.1"
                     /translation="MSPIARRGLCLVLSAPSGAGKTAISEALLAAEPALRRSVSVTTR
                     KPRPNEREGVHYFFREQAAFDSMAGSGALLEWAKVLGRDSYGTPRAAVEQALSEGRDM
                     IFDIDWQGYRQLRTALPEDVVGVFVLPPSMAVLEQRLRARAGDQPEEIARRMALAHDE
                     ISHWPEFDHVVVNHELTRAIADVTAILHAARLATARQPGLPAFIEALELGAG"
     gene            complement(1118..2455)
                     /gene="aceA"
                     /locus_tag="VFN42_04950"
     CDS             complement(1118..2455)
                     /gene="aceA"
                     /locus_tag="VFN42_04950"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HET8995996.1"
                     /translation="MRTIPTPTRAPDGLGAGAGSDRFAGIRRDYSPEDVARLSGSFRV
                     RHTIAEMGAARLWQLLRTQPFVPTLGALTGNQAVQQVKAGLDAIYMSGWQVAADANTA
                     GQMYPDQSLYPVDSVPNVVRRINNALRRADQIAQSEGGDGTHWMAPIVADAEAGFGGA
                     LNAHELMKAMIEAGAAGVHFEDQLASEKKCGHLGGKVLVPISQHIRTLNAARLAADVE
                     GVATVLLCRTDAQSAQLLTSDVDERDRPFLSGERTAEGFFRIKPGIGNDYAIARGLAY
                     APYSDLLWWETSEPDLHEAERFANAIHREFPGKMLAYNCSPSFNWKRKMTARQMESFQ
                     HDIGQMGYKFQFVTLAGFHSLNLSMFELSRAYRMRGMAAYSELQQAEFAAEPHGYTAT
                     RHQREVGVGYFDAVAMAITGGRSSTTALSGSTEAAQFHDDADAHIQEPALAAE"
BASE COUNT          391 a          843 c          835 g          405 t
ORIGIN      
        1 gaggagctgg accggctgca cagccatgtg caggcggcgc atgcgctgct gcgcgaggcc
       61 gccaatatcg gccgccgctt cgatttcctc gtgcaggagt tcaaccgcga agccaacacg
      121 ctgtgcagca agtcggcctc caccgcgctg accgcgaccg gcctgaagct gaaagcgacg
      181 atcgagcaat tgcgcgagca ggtgcagaac atcgaatgag cccgatcgcc cgccgcggcc
      241 tgtgcctggt gctgtcggcc cccagcggcg ccggcaagac cgccatctcc gaggcgctgc
      301 tggcggcgga accggcgctg cgccgttcgg tcagcgtcac cacgcgcaag ccgcggccga
      361 acgagcgcga gggcgtgcat tacttcttcc gcgagcaggc cgccttcgac tcgatggccg
      421 ggtcgggtgc cctgctggaa tgggccaagg tgctgggccg cgacagctac ggcacgccgc
      481 gcgcggcggt cgagcaggcg ctatcggaag gccgggacat gattttcgat atcgactggc
      541 agggctatcg ccagttgcgc accgcgctgc cggaggatgt ggtgggggtg ttcgtgctgc
      601 caccctcgat ggcggtgctg gaacagcgcc tgcgcgcccg cgccggcgac cagccggagg
      661 agatcgcccg ccgcatggcc ctggcgcatg acgagatcag ccactggccg gagttcgacc
      721 acgtcgtggt caaccacgag ctcacccgtg cgatcgccga tgtcacggcc atcctgcacg
      781 cggcgcggct ggcgaccgcg cggcagcccg gcctgccggc ctttatcgag gccttggagt
      841 tgggagcggg ctgagcccca cccacaccat cgccggcgtc gtcacccgca ccgtcatgac
      901 cggccatgca tcgagttccc ccccgtcata gccggcgaag gccggccatc catgacttgc
      961 tgcggaagac tcctgacgag gtccagacct cgacagcaag acgtgaatgg ccggccttcg
     1021 ccggccatga cggggaaagc cttcgccggc gacaacgggg aggccttccc cggccacgac
     1081 ggggaggctg ccgccgtccc cgcgcacgcc gtacggccta ctccgctgcc aacgctggtt
     1141 cctggatgtg cgcgtctgcg tcgtcgtgga attgcgctgc ctcggtgctg ccggacagcg
     1201 ccgtggtgga agaacgtccg ccggtgatcg ccatcgccac cgcgtcgaaa tagccgactc
     1261 ccacctcccg ctggtgccgc gtcgccgtgt agccgtgcgg ctccgccgcg aactcagcct
     1321 gctgcagctc ggaatacgcc gccatgccgc gcatccgata ggcgcgtgac agttcgaaca
     1381 tcgacaggtt caggctgtgg aatcccgcca gcgtgacgaa ctggaacttg tagcccatct
     1441 ggccgatgtc gtgctggaac gactccatct gccgcgcggt cattttccgc ttccagttga
     1501 agctgggcga gcagttgtat gccagcatct tgccggggaa ttcccggtgg atcgcattgg
     1561 cgaagcgctc cgcctcgtgc aggtccggct cgctggtttc ccaccacagc aggtcggaat
     1621 acggcgcgta agccaggccg cgcgcgatgg cgtagtcgtt gccgatcccc ggcttgatgc
     1681 ggaagaagcc ttccgccgta cgctcgccgg acaggaacgg acgatcgcgt tcatcgacat
     1741 cgctggtcag cagctgcgcg ctctgcgcgt cggtgcggca cagcagcacg gtggcaacgc
     1801 cttccacatc ggcggcaagc cgagcggcgt tcagtgtgcg gatgtgctgg ctgatcggca
     1861 ccaggacctt gccgccgaga tggccgcatt tcttttccga cgccagttgg tcctcaaaat
     1921 gcacgcccgc cgcaccggct tcgatcatcg ccttcatgag ttcatgggcg ttgagcgcac
     1981 cgccgaagcc tgcttccgca tcggcgacga tcggcgccat ccaatgcgtg ccgtcgccgc
     2041 cttcgctttg cgcgatctgg tcggcacggc gcagcgcgtt gttgatccgc cgcaccacgt
     2101 tcggcacgga gtcgaccgga tacagcgact gatcgggata catctggccg gcggtgttgg
     2161 catcggcggc aacctgccag ccggacatgt agatcgcgtc cagcccggct ttcacctgct
     2221 gcactgcctg gttgccggtc agcgcgccca gcgtcgggac gaagggctgg gttcgaagca
     2281 actgccagag gcgggcagcg cccatctcag cgatggtgtg gcgcacgcgg aagctgccgg
     2341 acaggcgagc gacatcctcc ggtgagtagt cgcggcggat accggcgaag cggtcggaac
     2401 cggcccccgc gcccaatcca tcgggggcgc gggtgggcgt cgggatggtg cgcatgattg
     2461 aactcctcgg gttg
//
LOCUS       DASSZH010000230         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1624604, whole genome shotgun sequence.
ACCESSION   DASSZH010000230 DASSZH010000000
VERSION     DASSZH010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624604"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VFN42_04955"
     CDS             complement(<1..593)
                     /locus_tag="VFN42_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET8995997.1"
                     /translation="MAQSDTRKRGKTRQSSADREPLLQAYRAMLLIRRFEEKAGQLYG
                     MGLIGGFCHLYIGQEAVVVGMQMAIEQGDEVVTSYRDHGHMLACGMDPRGVMAELTGR
                     ATGYSHGKGGSMHMFSKEKNFFGGHGIVGAQVSIGTGLGFSNWYRGNDRVALTYFGEG
                     ASNQGQVFESFNLAALMKLPVIYIIENNKYGMGTSVER"
     gene            931..1164
                     /locus_tag="VFN42_04960"
     CDS             931..1164
                     /locus_tag="VFN42_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995998.1"
                     /translation="MAEQKNEGEGNWTAARAYDRDQKQFAEQGKVDKAAQDAARAVDG
                     PEGEQLREAEQVGKSRAKGEDPALNKRAGGARP"
     gene            complement(1179..>2059)
                     /locus_tag="VFN42_04965"
     CDS             complement(1179..>2059)
                     /locus_tag="VFN42_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8995999.1"
                     /translation="WNHALPGITRAVIVIHGYLRNADAYYAVARQAVALAGPAGAGSI
                     LIAPQFLVDRDIAAHRLPPDVLRWSLIGWERGDNAQGPAPISSFAALDAIMGRLGDRR
                     RFPDLHSVVIAGHSGGAQVVQRYAILGRGSGLLPAGVAVRFVVANPSSYAWFNTQRPN
                     GKGGFAPFDAASCPRFNQWKYGMTDLPPYGVGADADALERAYLARDVIYLLGTADTNP
                     RQPALDRSCMAEAQGPNRLARGLAYVRYLRGHGGSAFHQHVLLVPGVGHNARRMLTSA
                     CGLAALFDRPGCASGR"
BASE COUNT          379 a          678 c          686 g          316 t
ORIGIN      
        1 cgctccaccg aggtgcccat gccgtatttg ttgttctcga tgatgtagat caccggcagc
       61 ttcatcagcg ccgccaggtt gaaactttcg aacacctggc cctggttgga cgcaccttcg
      121 ccgaaatagg tcagcgcgac gcgatcgttg ccgcggtacc agttgctgaa gcccaggccg
      181 gtgccgatgc tcacctgcgc gccgacgata ccgtgtccgc cgaagaagtt cttctccttg
      241 ctgaacatgt gcatggagcc gcccttgccg tgcgaatagc cggtcgcgcg cccggtcagt
      301 tccgccatca cgccgcgcgg gtccatgccg caggccagca tgtgaccgtg atcgcgatag
      361 gaggtgacga cctcgtcacc ctgctcgatc gccatctgca tgccgaccac cacggcctcc
      421 tggccgatgt acaggtggca gaagccgccg atcagtccca taccgtagag ctggccggct
      481 ttttcctcga accgccggat cagcagcatg gcacggtagg cttgcaacag gggctcccga
      541 tcagccgaag actgccgcgt cttgccgcgc ttcctggtgt ccgattgcgc catgaaactt
      601 cactccaccc cggattgccg gatcttgccc acacctagcc tcgcgggggc ggcggcgaca
      661 agtcgttgcc gggaaaaacc gtgattgtgc aatgcaatag cgtaaattaa ccacaatgtg
      721 gttaactggt tggcagatca tggctgcgtc ttcagctgcg gacgaccaat ttcaatggag
      781 ggccgctgtc cgacatgttc tttcgcgcgc gccggccaat acgcagcgat cctgccaggt
      841 gcccagccgg cccccaccgg ccacgcggaa cccgagccgg ccggatgagt tcctgccgag
      901 cccccgcagc gtgaaaaggg accgagcatc atggctgagc agaagaacga aggcgaaggc
      961 aactggacag ccgcccgcgc ctacgatcgc gaccagaagc agttcgccga gcaaggtaag
     1021 gtggacaagg cggcgcagga cgcggcgcgc gcggtcgacg ggccggaagg cgagcagctc
     1081 cgcgaggcgg agcaggtcgg caagagccgc gccaaggggg aagacccggc gctgaacaag
     1141 agggccggtg gcgctcgccc ctgacgtttc gccgcaggtc accggccgga tgcgcacccc
     1201 ggccggtcga acagggccgc caggccgcag gcagaggtca gcattcgccg cgcattgtgc
     1261 ccgacaccgg gcaccagcag cacgtgctgg tggaatgccg acccgccgtg gccgcgcagg
     1321 tagcgaacat aggcgaggcc gcgggccagg cggtttggcc cctgcgcctc ggccatgcag
     1381 ctacgatcca gcgccggttg gcgcggattg gtatcggcgg tgcccagcag gtagatgacg
     1441 tcgcgcgcca gataggcgcg ttccagcgca tcggcatcgg cccccacccc atagggcggc
     1501 agatctgtca tgccgtattt ccattggttg aaccggggac aggatgccgc gtcgaagggc
     1561 gcaaatccgc ctttgccgtt gggacgctgg gtgttgaacc aggcatagga ggacgggttg
     1621 gcgacgacga agcgcaccgc gacgccggcc ggcaagaggc cggaacctcg gcccaatatc
     1681 gcgtagcgct gcaccacttg ggcgccgccc gaatgtccgg cgatgaccac gctgtgcaga
     1741 tcgggaaaac gccggcggtc gcccaggcga cccatgatcg cgtccagcgc cgcgaaagag
     1801 ctgatcggcg ccgggccttg cgcgttgtcg ccgcgctccc agccgatgag cgaccagcgc
     1861 agaacgtccg gcggaagccg atgcgcggcg atgtctcggt ccacaaggaa ttgcggcgcg
     1921 atgagaatcg agccggcgcc ggccggtccc gccagggcga cggcttggcg agccaccgca
     1981 taataggcgt cggcgttccg cagatagccg tggatcacga tcaccgcgcg ggtgatcccg
     2041 ggcagtgcgt ggttccaat
//
LOCUS       DASSZH010000231         2922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_278115, whole genome shotgun sequence.
ACCESSION   DASSZH010000231 DASSZH010000000
VERSION     DASSZH010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278115"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VFN42_04970"
     CDS             <1..259
                     /locus_tag="VFN42_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996000.1"
                     /translation="SRGAGNLGQEQSMVSHKFAIGDRVQLVVDKYAGDVPPGVYMISR
                     RLPVEANICQYRVKHTQDGHERVVQESQLKPQLSSSVSQYK"
     gene            complement(226..786)
                     /locus_tag="VFN42_04975"
     CDS             complement(226..786)
                     /locus_tag="VFN42_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014457162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HET8996001.1"
                     /translation="MPDGGQHFPALVLNADFRPLSYFPLSLWSWQDAVKAVFLDRVSV
                     LSEYETEVRSPSLTMRLPSVIALKDYIPSARRPAFTRFNVFLRDAFHCQYCGDRYPAQ
                     DLTFDHVIPRSRGGRTTWENVVTACGECNLRKGSRLPRECHMFPRMHPRQPTTWELQD
                     NGRAFPPNYLHQSWRDYLYWDTELES"
     gene            complement(947..1600)
                     /locus_tag="VFN42_04980"
     CDS             complement(947..1600)
                     /locus_tag="VFN42_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET8996002.1"
                     /translation="MPPIDALYWGPASKSTPKQLVVLCHGIGADAHDLYELAPEWGAA
                     VPDALFASVNAPHPHPSGFGRQWWDVGDRSPAIMEAGVRGAALALDQFLDSELARLGL
                     PADAYALMGFSQGAMTVLFTGLRRAVAPRAILAFSGALLVPESLQRELRNKAPVLLVH
                     GEADETVPAGRSRDAESALQAAGVPVESSFIPGLGHGIDDTGLALGRLALQRAFGVA"
     gene            1638..2483
                     /gene="gluQRS"
                     /locus_tag="VFN42_04985"
     CDS             1638..2483
                     /gene="gluQRS"
                     /locus_tag="VFN42_04985"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003620888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HET8996003.1"
                     /translation="MRIVTRFAPSPTGYLHLGHAYAALFAWRRARDAGGAFLLRLEDI
                     DPGRCRPEFAAAILEDLAWLGLDWDGPVRVQSAHLHEYRAALDGLLARRLVYPCFCTR
                     ADIQREIAHSTSAPHAPDGSPLYPGTCRTLPDGERAARIAAGERFALRLDMTRALAAV
                     QLPLWFEELGEGRIACRPERFGDVVLARKDAPASYHLCVTHDDAVQGVTLVTRAEDLK
                     PATGLHRLLQALMGWPAPRYQHHGLLTDATGRRLAKRDRAATLRDLRAAGHTPAAVRA
                     MAEPR"
     gene            2592..>2922
                     /locus_tag="VFN42_04990"
     CDS             2592..>2922
                     /locus_tag="VFN42_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HET8996004.1"
                     /translation="MRHPITRVSRSPRRAAALLCLALLLPLPARAAEPGQALVIGEGS
                     YTGLPRLPACALSAHSVAASLRRLGFAVEEQEDATSGAMFAGIATLTRRIKAAPDAPV
                     FVYVCGYA"
BASE COUNT          442 a         1035 c         1007 g          438 t
ORIGIN      
        1 atcccgcggc gccgggaatc tcgggcagga gcagagcatg gtttcccaca agttcgcgat
       61 cggcgaccgg gtccagttgg tggtcgacaa gtatgccggc gatgtcccgc ctggcgtcta
      121 catgatttcg cgccggctcc cggttgaagc gaatatctgc cagtacagag taaagcacac
      181 gcaggacgga cacgagcgcg tggtgcagga gagccagttg aagcctcagc tctccagttc
      241 ggtatcccag tacaagtagt cacgccagct ctggtgcagg tagttgggcg ggaaggcccg
      301 gccgttatcc tgcagttccc acgtggtcgg ctggcgcgga tgcatccgcg ggaacatgtg
      361 gcattcgcgc ggcaggcgcg agcccttgcg cagattgcac tcgccgcagg cggtgacgac
      421 gttctcccag gtggtacggc cgccgcggct gcgcgggatc acgtggtcga aggtcaggtc
      481 ctgcgccggg tagcggtcac cgcaatactg gcagtggaag gcgtcccgca ggaacacgtt
      541 gaaccgggtg aaggccggcc gccgagcgga cgggatgtag tccttcagcg cgatcacgct
      601 gggcagccgc atggtcaggc tgggggagcg gacctcggtc tcgtattcgc tcagcactga
      661 gacgcgatcc aggaacaccg ccttcaccgc gtcctgccag gaccagagtg acagcggaaa
      721 ataggacagc gggcggaaat ccgcgttcag gaccagggct ggaaagtgtt ggccaccgtc
      781 aggcacgtcg cgctccctcg cgtccggagc gcaagcactg ggggagcgtt ggtcgccggc
      841 ccgccagata ttgtgcgccg tcgagaaccc agtcgtttat gacagagcca aacgggcgcc
      901 gcaaccgcca cgcgcgcgga ttcaccatgc tgtcatgccc cgcgcctcat gccacgccga
      961 aggcacgctg cagcgccagc cggcccagcg ccaggccggt atcgtcgatg ccatgcccca
     1021 gccccgggat gaagctggat tcgaccggca caccggcggc ttgcagcgcc gactcggcgt
     1081 cgcgcgagcg ccccgccggc accgtctcgt ccgcctcccc gtgcaccagc aagacgggcg
     1141 ccttgttgcg caactcccgc tgcagcgact cggggaccag cagcgcgccg gagaaggcca
     1201 ggatcgcgcg cggcgccacg gcgcggcgca atccggtgaa cagcacggtc atcgcaccct
     1261 ggctgaaccc catcagcgca taggcatcgg ccggcaggcc gagccgcgcc agttcggagt
     1321 ccaggaattg gtccagcgcc agcgcggcgc cgcgcacacc ggcctccatg atcgccgggc
     1381 tgcggtcgcc cacgtcccac cactgccggc cgaagccgga tgggtgcggg tgcggcgcgt
     1441 tcaccgaggc gaacagcgcg tcgggaaccg ccgcgcccca ttccggcgcg agctcgtaca
     1501 ggtcgtgcgc gtcggccccg atcccgtggc acagcaccac cagttgcttc ggtgtgctct
     1561 tggaagccgg cccccagtac agcgcgtcga ttggtggcat tctgctctcc gctgatgctg
     1621 cgatagtctt ctcccatatg aggattgtca cccgcttcgc acccagcccg accggctacc
     1681 tgcatctggg ccacgcctat gccgcgctgt tcgcctggcg gcgggcgcgg gatgccggcg
     1741 gcgcgtttct gctgcggctg gaggatatcg accccggccg ctgccggccg gaattcgccg
     1801 cggcgattct ggaggacctg gcgtggctgg ggctggactg ggatggcccg gtgcgggtgc
     1861 agtcggcgca tctgcacgag taccgcgcgg cgctggacgg cttgctggcg cggcgcctgg
     1921 tctatccctg cttctgcacg cgcgcggata tccagcggga gatcgcccac tccaccagcg
     1981 cgccgcacgc gccggacggc agcccgttgt atcccggcac ctgccgaacc ctgccggacg
     2041 gcgaacgcgc ggcacggatt gccgccgggg agcgcttcgc gctgcggctg gacatgacgc
     2101 gggcgctggc cgcggtgcag cttccgctgt ggttcgagga actgggcgaa ggccgcatcg
     2161 cctgccggcc ggagcggttc ggcgacgtgg tgctggcgcg caaggacgcg ccggccagct
     2221 accatttgtg cgtcacgcat gacgatgcgg tacagggcgt caccctggtg acccgcgccg
     2281 aggacctgaa gccggccacc ggactgcacc ggctgttgca ggcgctgatg ggctggccgg
     2341 cgccgcgcta ccagcaccac ggcctgctga ccgacgcaac cggccgccgc ctggccaaac
     2401 gcgaccgcgc agcgacgctg cgcgacctgc gcgccgccgg ccacacgccc gccgcggtgc
     2461 gcgccatggc ggagccgcgc tagccgatca cgctccgttt gtgttgccac gccggctccc
     2521 cccgtctaag cgcgaccgga tcgccgctcg ccggcaccac gcccagagca ttcatcgagg
     2581 aggcagggcc gatgcggcac cccataacgc gcgtgagccg gagtccgcgc cgtgcagccg
     2641 ccctgctctg cctggccttg ctgcttcccc tgcccgcccg cgctgccgaa ccggggcagg
     2701 cactggtgat cggcgaggga tcctacaccg gcctgcctcg gctgcccgcg tgtgcactct
     2761 ccgcccacag cgtcgccgcc agccttcgcc gcctcggttt tgcggtggag gagcaggaag
     2821 acgccaccag cggcgcgatg tttgccggta ttgccacgct gacccggcgg atcaaggcgg
     2881 ctccggatgc cccggttttc gtctatgtct gcggctacgc ca
//
LOCUS       DASSZH010000232         2561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_721279, whole genome shotgun sequence.
ACCESSION   DASSZH010000232 DASSZH010000000
VERSION     DASSZH010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721279"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..896
                     /locus_tag="VFN42_04995"
     CDS             141..896
                     /locus_tag="VFN42_04995"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET8996005.1"
                     /translation="MTALLFFLAGAFAWTLSSVAAGGGSMILAAAASLIAPGRAIAPV
                     ITVTSLIAAVVRIVLFRRLIVWRLVAWYLPGGIAGALAGSWIFASLRADWLQVVVGGF
                     LLSAVVQYQIAQRRHWRPMPPAGFIPVSFFSGLVSAVVGASGLLANPFYLGYGLEKQT
                     LLATRAVNSLAIQLTKLASYTAFGVLTGERLFDGLAASAGAAIAIAASNSWLERLSSK
                     RFRQLAVGLIVATGLLMLWRQRGLMLGLVGHIR"
     gene            893..1408
                     /locus_tag="VFN42_05000"
     CDS             893..1408
                     /locus_tag="VFN42_05000"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HET8996006.1"
                     /translation="MIADVAHAVLAWMQQHEAWAPAVVFALAFGESLAVISLLLPATV
                     ILLGAGGLIGAAGLSFWPVWTAAVAGAVLGDAVSYWVAYHFQDAVGRIWPLSRHPDLL
                     PRGHAFFQRWGTASVFLGRFFGPLRSVVPLVAGLCGMPHLMFQFANVTSALAWATLIL
                     APGVFGARWLL"
     gene            1419..2435
                     /locus_tag="VFN42_05005"
     CDS             1419..2435
                     /locus_tag="VFN42_05005"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012552930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HET8996007.1"
                     /translation="MPAPPPRANPLLLGHQAAETTLLEALRGGRMHHAWLITGPEGVG
                     KATLAFRFARRLLAGPPVRDSLELDPSHPVFRRVAAASHADLLTIERAYDPKRKRLRT
                     QIAVEDVRKINGFMALTPAEGGWRVVVVDGAEDMNQASANALLKVLEEPPPRAVLLLA
                     CAAPGRLLPTIRSRCRRLRLSALDDASMGTLLAGYLPDTPAEERERLVTLAEGSPGRA
                     MLLMEEEGIAIAGLVDKVLAELPNLPPMRAYEVADALGRSDTGFSTFMDLLRTGLAAA
                     VRDAARGRADPEQARLAGLRPLDAWGEVWHGLTRLQDETERFALDKRAALVQGLGLLS
                     GSRS"
     gene            2432..>2561
                     /locus_tag="VFN42_05010"
     CDS             2432..>2561
                     /locus_tag="VFN42_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007400377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET8996008.1"
                     /translation="MSGRYFITTPIYYVNGAPHIGHAYTSVAADVLARWKRLDGYDV"
BASE COUNT          338 a          861 c          902 g          460 t
ORIGIN      
        1 gacgcacttg tgggtaacga tcagcgcttt gcccgggcac ccacgtcttc ctgtggtgca
       61 cgcagcgaag acgtgggtgg ccgggccaag cccggccatg acacggaagg tgggtcaccg
      121 cgaaacccgg tcatcgaaca atgaccgcgc tgctgttctt cctggccggt gctttcgcct
      181 ggacgctgag cagcgtggcg gccggcggcg gcagcatgat tctcgcggcg gcggccagcc
      241 tgattgcgcc aggccgcgcc attgcgccgg tgatcaccgt caccagcctg atcgccgctg
      301 tcgttcgcat cgtgctgttc cgccgcctga tcgtctggcg gctggtcgcc tggtacctgc
      361 cgggcggcat cgccggcgcg ctcgccggaa gctggatttt tgcgtcgctg cgggccgact
      421 ggctgcaggt cgttgtcggg ggcttcctgc tgagcgccgt cgtccagtat cagatcgcgc
      481 agcgtcgcca ttggcggccg atgccgccgg ccggcttcat cccggtgtca ttcttctccg
      541 gcctggtctc cgccgtggtc ggcgccagcg gcttgctggc caacccgttc taccttggct
      601 acgggctgga gaagcaaacg ctgctggcca cccgggcagt gaattcgctg gcgatccaac
      661 tgaccaagct cgccagctac accgcattcg gggtcctgac cggtgagcgg ctgttcgacg
      721 ggctggcggc cagcgccgga gccgccatcg ccattgcggc gagcaattcc tggctggagc
      781 gattgagttc gaagcgcttc cggcaattgg cggtcggact gatcgtcgcc acgggcctgc
      841 tgatgctgtg gcggcagcgc ggcttgatgc tgggcctggt cggccacatc cgatgatcgc
      901 cgacgtcgcc cacgcagtac tggcatggat gcagcagcac gaggcctggg cgcccgccgt
      961 ggtgttcgct ctggcattcg gcgaatcgct ggcggtgatc tcgctgctgc taccggccac
     1021 ggtcatcctg ctgggcgcag gtgggctgat cggtgcggcc ggcctttcgt tctggccggt
     1081 atggaccgct gcggtggctg gggccgtgct cggcgacgca gtatcctact gggtggcata
     1141 tcacttccag gatgcggtcg ggcggatctg gcccttgagc cggcatccag acctgctgcc
     1201 acgcggccat gcgtttttcc agcgctgggg caccgccagc gtgttcctcg gccggttctt
     1261 cggtccgctg cgctccgtgg tgccgctggt cgccggcctc tgcggcatgc cccacctgat
     1321 gttccagttc gccaatgtca cctcggcgct ggcatgggcg acgctgatcc tggcgcccgg
     1381 cgtattcggc gctcgctggc tgctgtagcc tgcgccgcat gcccgcaccg cctcctcgcg
     1441 ccaaccccct gctgctcggc caccaggcgg ccgaaaccac cctgctggaa gcccttcggg
     1501 gagggcgcat gcaccacgcc tggctgatca ccggccccga aggcgtcggc aaggcgacac
     1561 tcgccttccg ctttgcccgc cggctgctgg ccggtccgcc cgtccgggac agcctggaac
     1621 tggatccgtc ccacccggtg ttccgccgcg tcgcggcggc gtcgcatgcc gacctgttga
     1681 ccatcgagcg tgcctatgac cccaagcgca aacgcctgcg cacccagatc gcggtggagg
     1741 atgtccgcaa gatcaatggc ttcatggcat tgacgccagc agagggcggc tggcgcgtgg
     1801 tggtggtgga tggcgcggag gacatgaacc aagcctccgc caatgcactg ttgaaggtgc
     1861 tggaagagcc gccgccgcgg gcggtgctgc tgctggcgtg cgccgcaccc ggtcggctgc
     1921 tgcccaccat ccgcagccgc tgccgccggt tgcgcctgtc cgcgctggat gatgccagta
     1981 tgggcaccct gctggcggga tacctgccgg acacgcccgc ggaggagcgc gagcggttgg
     2041 tgacgctggc ggagggctcg cccggccgag cgatgctgct gatggaggag gaggggatcg
     2101 cgatcgcagg attggtcgat aaggtgctgg cagaactgcc gaacctgcca ccaatgcggg
     2161 cctatgaagt ggccgacgcg ctgggccgca gtgacactgg gttctccacc ttcatggacc
     2221 tgctgcgcac cggtctggct gccgcggtac gcgacgcggc gcgtggcagg gcggacccgg
     2281 agcaggcgcg gctggccggg ctgcgcccgc ttgatgcctg gggcgaggtg tggcacggcc
     2341 tgacgcgatt gcaggacgaa accgagcgct tcgccctgga caagcgcgcg gcgctggtcc
     2401 agggcctggg gctgctcagc gggagcaggt catgagcggc cggtacttta tcactacccc
     2461 gatctattac gtgaatggcg cgccccatat cggccacgcc tatacctcgg tcgccgctga
     2521 cgtgctggcg cgctggaagc ggctggacgg ctacgacgtg t
//
LOCUS       DASSZH010000233         2609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_175875, whole genome shotgun sequence.
ACCESSION   DASSZH010000233 DASSZH010000000
VERSION     DASSZH010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175875"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..380
                     /locus_tag="VFN42_05015"
     CDS             222..380
                     /locus_tag="VFN42_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996009.1"
                     /translation="MVDRFADFRERVERAAEELLLPHPVDAGDDDNQAGVQRLRRKRP
                     VSPAWVAS"
     gene            complement(410..2068)
                     /locus_tag="VFN42_05020"
     CDS             complement(410..2068)
                     /locus_tag="VFN42_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HET8996010.1"
                     /translation="MPAPLDTATLPDNVIALRSLLLAREAEHAAEREQQTSELQAARE
                     GLKAQVLRNEQLKLRLSRLLRERFGASSEKLRGTLEQLELLLGDLEEQIAETSPAEPE
                     PQAAEADAARRRKPARRPLPESLPREVVEHAAPCTCPQCGGALHPLGEDVTEVLDYVP
                     GTFRVIRHVRPKLSCRSCESIAQAPAPELPSRLTALLGISPLRGLIRRGLAAAGMLAH
                     VLVAKYCDHLPLYRQAEIYARNGVDLDRSTLADWVGQTAALVRPLVEAVGAHVMAAER
                     VHADDTTVPVLEPGRGRTKTGRLWCYTRDDRPFAGQAPPAVLYRYSPDRKGEHPRAHL
                     AAFHGILQADGYAGFAGLYGRGVTEAACWAHARRKFFDAQTGEPLPIATEVMQRIAVL
                     YAVEATIRGQSPEARLQARKQHSAPLFDVLKDFLDRSLRRISGKSDLAGAIRYTLSRW
                     EALTLVLRDGRACIDNNAAERAMRPMALGRKSWLFAGSDAGGERAAAIYTLTETAKLN
                     GLDPEDYLRQVLDRIAEHPVKRVHELLPWNLAGIRARLDQRKAA"
     gene            complement(2118..2465)
                     /gene="tnpB"
                     /locus_tag="VFN42_05025"
     CDS             complement(2118..2465)
                     /gene="tnpB"
                     /locus_tag="VFN42_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183759.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HET8996011.1"
                     /translation="MIAPPPGVHVWLAAGVTDMRKGFDGLSALVQQRLGQDPFSGQLF
                     VFRGRRGDLVKVLSWDGQGLCLYAKRMEKGRFVWPQAKDGAVSLTAAQLSMLLEGIDW
                     RMPVRSWRPEMAG"
BASE COUNT          399 a          921 c          841 g          448 t
ORIGIN      
        1 cgccagccac cgcatgcgcc attcgtcaat caacgccgcc gtgccgtggg ccatgacagg
       61 tgcgtccgcg cccaccggcc cgtgtcgcca gcggcctctt cctccaccat tgcccgatcg
      121 cttcctacca gttcggtcga atggatcgac tcagttgtca gccgccggaa actcatagag
      181 gcggaacgtc gcttcgtcgc cctcgattcc acttccagca catggttgat cggttcgccg
      241 atttccgcga gcgcgtcgag cgcgccgcgg aagagctgct gttgccgcat ccggttgacg
      301 ctggggatga tgacaaccag gctggcgtgc agcggctgcg tcgtaagcgc ccggtcagcc
      361 ccgcctgggt cgcctcctga gggccgggta tgatgtgggt cgatgtggat caggcagctt
      421 tgcgctgatc cagcctcgcc cggatgccgg cgaggttcca cggcaacagt tcatggaccc
      481 gcttgaccgg atgttctgcg atacgatcca gcacctggcg gaggtaatcc tcgggatcga
      541 gcccgttcag ttttgccgtc tcggtcagcg tgtagatcgc agcggcccgc tcgccgccgg
      601 catcggaccc ggcgaacaac cagctcttgc gtcccagcgc cattggccgc atcgcgcgtt
      661 cggcagcgtt gttgtcgatg caggcgcggc cgtcgcgcaa caccagcgtc agtgcttccc
      721 agcgcgacag ggtgtagcga atggcaccgg ccaggtcgct cttgccggag atccgccgca
      781 ggctgcggtc cagaaaatcc ttcaacacat cgaacagcgg tgcgctgtgc tgcttgcggg
      841 cttgcagccg cgcctcgggc gattggccgc ggatcgttgc ttcgactgca tacaggacgg
      901 cgatccgctg catcacctcg gttgcgatcg gcagcggctc gccagtctgc gcatcgaaga
      961 acttgcgccg ggcgtgtgcc cagcacgcgg cttccgtgac gccgcgcccg tagagcccgg
     1021 cgaagccggc gtagccgtca gcttgcagga tgccgtggaa ggctgcgagg tgggcgcgcg
     1081 gatgttcgcc cttgcggtcg gggctgtagc gatacagcac ggcgggcggc gcttgccccg
     1141 cgaatggccg gtcatcgcgc gtgtagcacc acaatcgtcc ggttttggtt ctgccgcggc
     1201 ccggctcgag caccggcacc gtcgtgtcgt cggcatgtac tcgctcggcc gccatcacat
     1261 gcgcgccgac cgcctcgacc agcgggcgca ccagcgccgc cgtctggccg acccagtcag
     1321 ccagcgtgga tcggtccaga tcgacgccgt tgcgggcata gatctccgcc tggcgataca
     1381 acggcaggtg gtcgcagtac ttcgccacca gcacgtgcgc cagcatgccg gcggcggcca
     1441 ggccccggcg gatcaggccg cgaagcggac tgatgcccag caaagcggtt agccggctcg
     1501 gcagctccgg cgccggcgcc tgggcgatgc tctcgcagct gcggcacgac agcttcggcc
     1561 gcacgtggcg aatgacgcgg aacgtgcccg gtacataatc caacacttcg gtgacgtcct
     1621 cgccgagcgg atgcagtgca ccgccgcatt gcggacaggt gcagggagca gcgtgctcga
     1681 cgacctcgcg cggcaggctc tccggcaacg gccggcgtgc cggcttgcgg cgcctggctg
     1741 cgtccgcctc ggcagcctgt ggttccggct cagccgggct ggtctcggcg atctgctctt
     1801 ccaggtcacc gagcagcagc tcgagttgct cgagcgtgcc gcgcagcttc tccgaggacg
     1861 caccgaaccg ctcacgcagc agccggctca ggcgcagctt cagctgctca ttgcgcagca
     1921 cctgcgcctt cagcccctcg cgcgcggctt gcagttcgct cgtctgctgc tcgcgttcag
     1981 ctgcgtgctc cgcttcgcgt gccagcaaca ggctgcgcaa cgcgatcacg ttgtccggca
     2041 gcgttgcggt gtcgagtggg gctggcatgc gcgaatcctc ctccatcgat tcgcgtcgct
     2101 ccaagaactt tctgcatcta tcctgccatt tccggacgcc agctgcgcac tggcatgcgc
     2161 cagtcaatgc cttccagcag catcgacaac tgagccgcgg tgagtgacac cgcaccatcc
     2221 ttcgcctgcg gccagacgaa gcggcctttc tccatgcgct tggcataaag acacaggccc
     2281 tggccgtccc agctcagcac cttcaccagg tcgccgcggc gaccgcggaa cacaaacagc
     2341 tggccactga acggatcctg gccaaggcgc tgctgcacga gcgccgacag cccgtcaaag
     2401 cctttgcgca tgtcggtcac accggctgcc agccacacat gcacgcccgg cggcggcgcg
     2461 atcatccgcg cagtgccgct atcacacggc gcagtgtcgc cgcgtggacg ccgttgccga
     2521 cccgcaggcg acagccgtca ggcagttcga tctcgatcgt ctcggcgctc cgctggatcg
     2581 aagccgaacg ctgcgccggc acagtcgtg
//
LOCUS       DASSZH010000234         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_124766, whole genome shotgun sequence.
ACCESSION   DASSZH010000234 DASSZH010000000
VERSION     DASSZH010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124766"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VFN42_05030"
     CDS             complement(<1..607)
                     /locus_tag="VFN42_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8996012.1"
                     /translation="MSDNALPPESGQEPDAYDAASISVLKGLDAVRRRPGMYIGDTDD
                     GSGLHHMAFEIIDNAVDEAQAGFARNVDVVLNGDGSVTVRDDGRGIPTDIHAEEGISA
                     AEVVLTRLHAGGKFNQNSYKVSGGLHGVGAAVVNALSEWMDVRIWRHGQEHFIRFRHG
                     DAEAPLAVVGPTERTSGTEVTFKPSPATFTKTEFDYALLERR"
     gene            complement(658..1821)
                     /gene="recF"
                     /locus_tag="VFN42_05035"
     CDS             complement(658..1821)
                     /gene="recF"
                     /locus_tag="VFN42_05035"
                     /inference="COORDINATES: protein motif:HMM:NF000820.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HET8996013.1"
                     /translation="MRLLRLSLTDFRNYATLTWRPRARIAVLVGPNGSGKTNLLEAVS
                     LLVPGRGLRNARNADLPRHDGAGGWAVAGRIAATDGEIDLGTGTLPDGPPERRVFRID
                     GVTPRSQAEIAGLLAAAWLTPQMDRLFLEPLSGRRRFLDRLVWALEPAHAREVAAHDA
                     AMGSRNRLLAERRDESSWLAGLEDAMARHAVAATAARMTLVARLNAVLETGITGAFPP
                     VRIALACPIAERLAVAPALTVEDWLRSSLAGGRGRDAAAGGAALGAHRTDLRLSDANG
                     TPAEQASTGEQKAMLVGLVLAHAALIAEARGFAPLLLLDEPAVHLDAIRRQALFDALL
                     RLPAQTLITGTDPDIFAPLAGRIEMLRTGSGTLLPEPRFPLPEASCSPVPGAA"
BASE COUNT          375 a          712 c          690 g          326 t
ORIGIN      
        1 gccgccgctc gagcagcgcg taatcgaatt cggttttggt aaaggtcgcc gggctcggtt
       61 tgaacgtgac ctcggttccg cttgtccgct ccgtcgggcc gaccacagcc agcggtgcct
      121 cggcatcacc gtggcggaag cggataaagt gctcctggcc gtggcgccag atgcgcacat
      181 ccatccattc agacagtgcg ttgaccacgg cagcgccgac accgtgcagc ccgccggaga
      241 ccttatagga gttctggttg aacttcccac cggcatgcag ccgcgtcagc actacctccg
      301 cggcggaaat cccctcttcg gcgtggatat cggtggggat cccacggccg tcgtcgcgca
      361 ccgtgacgct gccgtcaccg ttcaggacaa catcgacatt gcgggcgaag ccggcctgcg
      421 cctcatccac ggcgttgtcg atgatctcaa acgccatgtg atgcaggccc gagccgtcat
      481 cggtatcgcc gatatacatt ccgggccggc ggcgaaccgc atccagcccc ttcaggaccg
      541 agatcgaggc tgcgtcatag gcgtctggct cttggccgga ctcaggcggc aaggcattgt
      601 cggacatgcc gaggtgcgac tccatgcggt gcggaaggta ctctagatat atagcgtcta
      661 cgcggcccca ggcacagggg aacaggacgc ttccggcaag ggaaatcgcg gttccggcag
      721 caaggtgcca ctgccggtgc gcagcatctc tatccggccg gcgagtggcg caaatatgtc
      781 cgggtcggtg ccggtgatca gggtctgcgc cggcagccgg agcagggcat cgaacagcgc
      841 ctggcggcgt atggcgtcca aatgtaccgc cggttcgtcc agcaacaaca acggggcgaa
      901 gccgcgagcc tcggcaatca gcgcggcatg ggccagcacg aggccgacca acatggcctt
      961 ttgctcccca gtactggcct gttctgccgg cgtgccgtta gcgtccgaca agcgcaggtc
     1021 ggtacgatgg gcgcccagcg cggccccacc ggcagccgca tcgcgtccgc ggccgccggc
     1081 caggctgctg cgcaaccagt cctctacggt caaggccggg gcgacagcca ggcgctcggc
     1141 aatcggacag gccagggcga tgcgcacggg cggaaaagcg ccggtaatgc ccgtctccag
     1201 caccgcgttg agccgggcca ccagggtcat gcgagcggcc gtggctgcga cggcgtggcg
     1261 ggccattgca tcctccaggc ccgccaacca ggacgactcg tcccggcgtt ccgccaacag
     1321 gcgattgcgg ctgcccatcg ctgcatcgtg cgccgccact tcccgggcat gcgctggttc
     1381 caaggcccag accagacggt ccaggaagcg cctgcgtccg gacagcggtt ccaggaacaa
     1441 ccggtccatc tgcggggtaa gccaagccgc ggcgagcagg ccggcgatct ccgcctgact
     1501 gcgcggcgtc acgccatcga tccggaaaac ccggcgctcc ggcggaccat ccggcagcgt
     1561 gccggtgccg aggtcgatct ccccgtcggt ggcggcgata cgcccggcaa ccgcccagcc
     1621 ccctgcccca tcatgccgcg gcaaatcggc gttgcgggca ttgcgcaaac cgcggcccgg
     1681 taccagcagg gaaacggcct ccagcagatt ggtcttgccg ctgccgttcg gcccgaccaa
     1741 cacggcgatg cgcgcgcgcg gacgccaggt cagcgtcgcg tagttgcgga aatcggtcag
     1801 ggacaggcgc aacaggcgca tcggacggtt ctgtggcggg tgcatggggt ggcgtcaacg
     1861 ttcgccatcc agccgcttcg gaaaaatcgt cggacgtgga agaaagtcct tgaccagatg
     1921 ccccatcttc ggctataaat ctggctatca tggcggggta aggcgatgca ccaccccatc
     1981 ggccttaggc cgcggtgccc gcgttccgcc aatctgagct gagcaagatc aaagcacgat
     2041 gcgacgaatg cccgagacag agtcgggatt cgtcccgccg tttccggata ccgccggcca
     2101 aac
//
LOCUS       DASSZH010000235         2781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1641704, whole genome shotgun sequence.
ACCESSION   DASSZH010000235 DASSZH010000000
VERSION     DASSZH010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641704"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /gene="gpt"
                     /locus_tag="VFN42_05040"
     CDS             complement(<1..460)
                     /gene="gpt"
                     /locus_tag="VFN42_05040"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019086624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HET8996014.1"
                     /translation="MAAHYYTVTWDQLHRDGRALAWRLIERGPFSGVVAITRGGLIPA
                     AIIARELDIRLVETVSVLTYGGARTGTLESERTIGEPVVLKPPSAAGDGEGFLLVDDL
                     VDTGTTARLVRKLLPRAHFACIYAKPAARDVVDSFVTEVSQDTWILFPWDT"
     gene            complement(599..1066)
                     /locus_tag="VFN42_05045"
     CDS             complement(599..1066)
                     /locus_tag="VFN42_05045"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8996015.1"
                     /translation="MLLQRDTTIRPAGPADARQVGDVFLAARAEALPRLRPQLSEAET
                     RDYLARLIDQSKHAVWVAERDGHVVGFLALRESWVDHLYVHPDYYRNGIGTRLLDCAK
                     QARPRGLRLYCFHCDQRAQAFYRARGLVAAGFAGGAGNAEPQPDVEYQWKPAA"
     gene            complement(1172..2089)
                     /locus_tag="VFN42_05050"
     CDS             complement(1172..2089)
                     /locus_tag="VFN42_05050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019091617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET8996016.1"
                     /translation="MRILFVTSNRLGDAVLSTGLLDHLLRSYPDARFTVACGPVAEGL
                     FERMPRRERTIVLRKQPYGRHWLPLWAATVTTRWDLVVDIRGSALAWMVPARRRAVMR
                     RVRGHKTTQLAAVLGLDPPPLPVVWTAPADEARAAALLPEGRPVIALAPTANWGPKVW
                     PPERFAALFAALRRDLDDPAAVVLAGPGEAERMMAEPLLAALPGAIDLTGRLRLPEAA
                     AVLRRAALFVGNDSGLMHLAAAAGAPTLGLFGPTKAAEYGPAGPYAAAVLADTGGMDT
                     LPVARALAAARTLLGHTMQPRTERPVPGR"
     gene            2190..>2781
                     /locus_tag="VFN42_05055"
     CDS             2190..>2781
                     /locus_tag="VFN42_05055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET8996017.1"
                     /translation="MSEATMVALGEDYPELRDSVRRICARYPGTYWSGLEDAEAYPTA
                     FVNELTEAGFLAALIPEEYGGSGLPLRAAAVILEEINASGCVASQGHAQMYIMGTLLR
                     HGSEAQKRKYLPEIAAGRLRLQAFGVTEPTTGSDTTQLKTRAVRDGDGYVVTGQKVWT
                     SRALHSDMMLLLARTTPVDQVRKRSDGLSVFLIDLSR"
BASE COUNT          440 a         1010 c          959 g          372 t
ORIGIN      
        1 cggtgtccca ggggaacagg atccaggtat cctgcgacac ctcggtgacg aagctgtcca
       61 ccacgtcgcg cgccgccggc ttggcgtaga tgcaggcaaa atgcgcccgc ggcagcagct
      121 tgcgcaccag gcgcgcggtg gtgccggtgt ccaccaggtc gtccaccagc aggaagccct
      181 cgccgtcgcc cgccgcgctc ggcggcttca gtacgaccgg ctcgccgatg gtccgctcgc
      241 tttccagcgt gccggtccgt gcgccgccat aggtcagcac ggacaccgtc tccaccagcc
      301 ggatgtcgag ttcgcgtgcg atgatcgccg ccgggatcag cccgccgcga gtgatcgcca
      361 ccacgccgct gaacggaccg cgttcaatca ggcgccaggc caaggcccgg ccgtcgcggt
      421 gcaactggtc ccaggtgacg gtgtaataat gcgctgccat gcccgcatcc tgcggattgt
      481 cgcacagagg cgcaagccgg cctggtgcgc gtcaacgccg cggttcggcc gcctggcagg
      541 cgcgccgcgg ggtgatgcgc cgccaccgta cggaccggcg cgtgccccca cgcggtcgtc
      601 aggccgccgg cttccactgg tactcgacat ccggctgtgg ttccgcattc ccggcaccgc
      661 ccgcaaaccc cgccgcgacc aagccgcgcg cccggtagaa cgcctgggcc cgctggtcgc
      721 agtggaaaca gtacagccgc agcccccgcg gccgggcctg cttcgcgcag tccagcaacc
      781 gcgtgccgat gccgttgcga tagtagtcgg gatgcacata caggtggtcc acccagctct
      841 cccgcagcgc gaggaatccc accacgtggc catcgcgttc ggccacccac accgcgtgct
      901 tcgactggtc gatcaaccgg gcgaggtaat cgcgcgtttc ggcttcgctc aactgcgggc
      961 gcagccgcgg cagggcctcg gcacgggcgg cgaggaacac gtcgccaacc tgccgggcat
     1021 ccgccggtcc tgccggccgg atcgtggtat cgcgctgcaa aagcacgggg aaacgctcct
     1081 tgggttgacg ggttagacgc agagccgaac cgcgcagccg tgcccgcggc acgaccacat
     1141 cccgtcaaga gttggcggaa aacggccgca gtcaccggcc aggaacgggg cgttccgtgc
     1201 ggggttgcat cgtatgacca agcagtgtcc gggccgcggc cagcgcgcgg gcaacgggca
     1261 gcgtgtccat gccgccggtg tccgccagca ccgccgccgc gtaagggccg gctggcccgt
     1321 actccgcggc cttggtgggc ccgaacagcc ctaatgtggg ggcgcccgcc gccgccgcca
     1381 gatgcatcag tccggaatcg ttccccacga acagcgcggc tcgtcggagc acggcggccg
     1441 cttccggcag gcggagccgg ccggtcaggt cgatggcgcc gggcagcgcg gccagcagcg
     1501 gttcggccat catgcgctcc gcctcgccgg gcccggcgag caccaccgcg gccggatcgt
     1561 ccagatcccg ccgcaaggcc gcgaacagcg ccgcgaagcg ctcgggcggc cagaccttcg
     1621 gcccccagtt ggcggtaggc gccagcgcga tcaccggccg gccctccggc agcagcgcgg
     1681 ccgcccgcgc ctcgtccgcc ggcgcggtcc acaccaccgg cagcggcggc gggtccaggc
     1741 caagcaccgc ggcaagctgc gtggtcttgt gaccccggac gcgccgcatc accgcgcggc
     1801 ggcgcgccgg caccatccag gccagcgccg acccgcggat gtccaccacc aggtcccacc
     1861 gcgtcgtcac cgttgccgcc cacagcggca gccaatgccg gccatagggc tgcttgcgca
     1921 gcacgatggt gcgctcgcgc cgcggcatcc gttcgaacaa accttcggcg accgggccac
     1981 aggccacggt gaaacgcgcg tcggggtaac tgcgcagcaa gtggtccagc agcccggtgg
     2041 acagcacggc gtcgccgagc cggttggagg tgacgaacag gatgcgcatt gcccccgccc
     2101 catagcagcg ttgcacgacc ggcgcgaggc aaccgtttga cgctccgcac gcggttgggc
     2161 agcgtgcccc gctcagcacg gaggggatca tgtcggaagc cacgatggtc gcgctcggcg
     2221 aggactatcc cgaactgcgc gattcagtgc gccgcatctg cgcgcgctac cccggcacct
     2281 attggagcgg cctggaggat gcggaggcgt accccaccgc cttcgtgaac gaactgaccg
     2341 aggccggttt cctcgccgcg ctgatcccgg aggaatacgg cggctccggt ctgccgctgc
     2401 gtgccgcggc ggtgatcctg gaggagatca acgcctccgg ctgcgtcgcc agccaggggc
     2461 acgcgcagat gtacatcatg ggcacgctgc tgcggcatgg cagcgaggca cagaagcgga
     2521 agtacctgcc ggaaatcgcc gccggaaggc tgcggctgca ggcgttcggc gtgaccgaac
     2581 cgaccaccgg ttcggacacc acgcagttga agacccgcgc ggtgcgcgac ggtgatggat
     2641 atgtggtgac aggccagaag gtgtggacgt cccgcgcgct gcattccgac atgatgctgc
     2701 tgctggcacg caccacgccg gtggatcagg tgaggaagcg cagcgacggg ctttccgtct
     2761 ttctgatcga tctgagccga a
//
LOCUS       DASSZH010000236         4151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1334794, whole genome shotgun sequence.
ACCESSION   DASSZH010000236 DASSZH010000000
VERSION     DASSZH010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334794"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..1093)
                     /locus_tag="VFN42_05060"
     CDS             complement(221..1093)
                     /locus_tag="VFN42_05060"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET8996018.1"
                     /translation="MRDASELAGLQRYPLARRSLMTTGMIGSLALATQSVEAQAIHTD
                     AAGLETGEVRIESDGAQLPAYFARPATGTGFPVVLVFEEIFGINDYLKDVCRRFAHLG
                     YFAVATEYYARLGDLSKMTDGKQIMQQVIAKAPDAQYMRDADATAAWAGKHKGDLGRL
                     GVNGFCRGGRQTWLYAAHNPHLKAAVSWYGVLKTPISPIQPETALQAVTQIRCPVLGL
                     YGAQDASNPLSVIQDAQAEAKHDREPVEIVVYPDAPHGFHADYRPSYRKDAAEDGWGR
                     AIAWFHRFGVKPTP"
     gene            complement(1169..2149)
                     /gene="rfaD"
                     /locus_tag="VFN42_05065"
     CDS             complement(1169..2149)
                     /gene="rfaD"
                     /locus_tag="VFN42_05065"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019087898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HET8996019.1"
                     /translation="MILVTGGAGFIGSNLQAALVRRGHETIIADWLGSEGKWRNLAAH
                     PPMRVIPPGELDSFLESRPPLEMVFHMGAISETTVTDADLTWATNVELSRRLWEWCAD
                     RGVRFVYASSAATYGDGAQGFEDDFTLPALQRLRPLNLYGWSKLAFDRLVAQKLAAHQ
                     PHPPQWAGLKFFNVYGPNEYHKGRMISVIKVKYDELAAGRPARLFRSDLPGLADGAQA
                     RDFIWVGDVVDVMLWLLENPAANGLFNVGTGRARSYLDMAHAVCDAAGLPRRVEFIDM
                     PANLRGQYQSFTQAPMDRLRSAGYAGQFTTLEEGVRRYVQDYLAGGDPYL"
     gene            2214..2510
                     /locus_tag="VFN42_05070"
     CDS             2214..2510
                     /locus_tag="VFN42_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014106115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HET8996020.1"
                     /translation="MSDRPRFFDDLAGVAGGAISALAGLREEVEAIARARIDETIRKL
                     ELVRREDFDAVAELAANARAGQEAAETRLNEALSRLTELEARMAALEAASSGSE"
     gene            2643..3146
                     /locus_tag="VFN42_05075"
     CDS             2643..3146
                     /locus_tag="VFN42_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HET8996021.1"
                     /translation="MSASLTTSFDPVSNPLDVMEQIIAANDWAFDRRSDSEMAAEAPG
                     RWCDYGLYFNWSNEISAMHFTCTFDMKVPERQRAMLYELLALANERLWIGHFGLETED
                     GMPVFRHAVLLRGAPGASAESLEDMVDIALTECERFFPAFQFVLWGGKTPQEALAAAM
                     LECAGEA"
     gene            complement(3205..>4151)
                     /locus_tag="VFN42_05080"
     CDS             complement(3205..>4151)
                     /locus_tag="VFN42_05080"
                     /inference="COORDINATES: protein motif:HMM:NF039581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HET8996022.1"
                     /translation="GTAATVTDANLVLGYLDPGNFLGGRRQLDLAAATRAIQAVAQPL
                     GIGVPEAASGIHRLVNSRMADGVRVATVRRGVDPRGYTLLAFGGAAGLHVTAVAAELG
                     IGRVVVPVAASVLSAWGMLNTDLRIELTRSQSQTRGIDPDALRAAFAAMEQEGRARLT
                     WFDGEIAVRRAADMRYGEQVFEIPVPLDNVDLHAPSVVAAIEAAFHQAHEALYTYALR
                     DQDVVLVNARVSVIGRLPPAPAASGSAPKQGDVPARSRRVFLDGWVEIPVFDFAALSG
                     DQTVAGPAIIESDTTTVLLRPGNQARFDRRGWLDVALL"
BASE COUNT          679 a         1409 c         1414 g          649 t
ORIGIN      
        1 accacgcggc gttcggcccc ctgcacgatg acactgtgcg aatacccgct cggcgcgcgc
       61 acggtggaag gattgcgggt ggtgatcacg ggcatggacg gactcccctg tggttcggcc
      121 gtgcgttggc gtgagcggcc cgggccactc tgcccgaaat cgccgcgcga ggcgatggcg
      181 tctcagtggt gcgcgctccg ctgcggcgcg cggcaaggtc tcagggcgtt ggcttcacgc
      241 cgaaccgatg gaaccaggcg atggcgcggc cccagccgtc ctccgccgca tccttgcgat
      301 agctgggacg gtaatcggcg tggaacccgt gcggcgcgtc cggatagacg acgatctcca
      361 ccggttcgcg gtcgtgcttc gcctcggcct gcgcatcctg gatgacgctg agcggattgc
      421 tggcatcctg ggcgccatac aggcccagca ccggacagcg gatctgcgtc accgcctgca
      481 gcgcggtctc cggctggatc gggctgatgg gcgtcttcag cacgccgtac cagctcaccg
      541 ccgccttcag gtggggatta tgcgcggcat acagccaggt ctgccgccca ccgcggcaga
      601 acccgttcac ccccagccgg ccgaggtcgc ccttgtgctt cccagcccag gcggcggtgg
      661 cgtcggcatc acgcatgtac tgcgcgtcgg gcgccttcgc gatcacctgc tgcatgatct
      721 gcttcccgtc agtcatcttc gacaggtcgc ccaaccgcgc atagtactca gtggccacgg
      781 cgaaatagcc cagatgggcg aagcggcggc agacatcctt gaggtagtcg ttgatgccga
      841 agatctcctc gaacaccagg accaccggaa atcctgtgcc ggtggcgggc cgggcaaagt
      901 aggccggcaa ctgcgcgcca tccgattcga tgcgcacctc cccggtctcc aggccggcag
      961 catcggtgtg gatcgcctgg gcctccacag actgcgtcgc caaagcgagg ctgccgatca
     1021 tgccggtggt catcaggctg cggcgggcga gcggatagcg ctgcagcccc gcgagttcgc
     1081 tggcatcacg catggcatcc atccctgatt gttatccgtc aaaagcgtag cggctaaggg
     1141 atatggcgca agccagccga ggcgttgctc agagatacgg atcgccgccc gcaaggtagt
     1201 cctgtacata gcgccgtacc ccctcctcca aggtggtgaa ctgaccggcg tagccggcgg
     1261 agcgcaggcg atccatcggg gcctgggtga aggactggta ttgcccgcgc aggtttgccg
     1321 gcatgtcgat gaattccacc cgccgcggca ggccggcggc gtcgcacacc gcatgcgcca
     1381 tgtcgaggta actgcgggct ctgccggtgc cgacgttgaa caggccgttg gcggccgggt
     1441 tctccagcag ccacagcatc acgtccacca cgtcgcccac ccagatgaag tcgcgcgcct
     1501 gggcgccgtc ggccagtccc ggcaggtccg aacggaacag ccgagccggg cggcccgccg
     1561 cgagttcgtc atatttgacc ttgatgaccg agatcatgcg gcccttgtgg tattcgttgg
     1621 ggccatagac gttgaagaac ttcagtcccg cccattgcgg cgggtgcggc tggtgcgccg
     1681 ccagtttctg cgccaccagc cggtcgaaag cgagcttgct ccagccatac aggttcagcg
     1741 gccgcagccg ctgtaaggcc ggcagggtga agtcgtcctc gaagccctgc gccccgtcgc
     1801 cataggtcgc cgccgaggat gcatagacaa accgcacgcc gcgatcggca caccattccc
     1861 acagccggcg gctcagctcg acattggtgg cccaggtgag gtcggcgtcc gtcaccgtgg
     1921 tctcgctgat ggcgcccatg tggaacacca tctccagcgg cgggcggctc tccaggaagc
     1981 tgtccagctc gccggggggg atgacacgca tcggcggatg ggcggcgagg ttgcgccact
     2041 tgccctcgct gcccaaccag tcggcgatga tggtttcgtg cccgcgccgc accagcgccg
     2101 cttgcagatt cgagccgata aagccggcgc cgcccgtgac caggatcatg cggcgacctt
     2161 atatggaggg cagacccgtc gcggaagccg gcggccgaac aggaggcact tgcatgagcg
     2221 atcgtccacg tttcttcgac gatctggccg gcgtggccgg cggggcgatc tcggccctgg
     2281 ctgggctgcg cgaggaggtg gaggccatcg cccgggcccg gatcgacgag acgattcgca
     2341 agctggagct ggtccggcgg gaggatttcg acgccgtggc ggaactcgcc gccaatgccc
     2401 gcgccggcca ggaagcagcg gaaacccgct tgaatgaggc actttcgcgg ctgacggagc
     2461 tggaggcccg catggcggca ttggaagcgg cgtccagtgg ctcggagtaa tgcttgcggc
     2521 gcggccaatt ggctcggtag gctaggaacc gcgggtaacc cagcgcgaat cctcgggatt
     2581 ttggattcgc acggcgtgct gggggactcg cgccaggcct tcctgcagat gggagacccc
     2641 gcatgtcagc ctcgctgacc acatccttcg accccgtgtc caatccgttg gacgtgatgg
     2701 aacagatcat tgccgccaat gactgggcct ttgatcgccg cagcgattcg gaaatggcgg
     2761 ccgaagcgcc cggccgctgg tgcgattacg gcctgtattt caactggtcg aacgagatca
     2821 gcgccatgca cttcacctgc accttcgaca tgaaggtgcc ggaacgccag cgggccatgc
     2881 tgtacgagct gctggcgctg gccaacgagc ggctgtggat cggccatttc gggctggaga
     2941 ccgaagatgg catgccggtg ttccgccacg ccgtgctgct gcgcggggcg ccaggcgcct
     3001 cggcggagag cctggaggac atggtggaca ttgcgctgac cgagtgcgaa cgcttcttcc
     3061 cggcgttcca gttcgtgctg tggggcggca agaccccgca ggaggcgctt gccgccgcca
     3121 tgctggagtg cgccggagag gcataggcgc cgcgaagcat gacgctggca ccggggctgg
     3181 cagcgccccg gccgccttgc ggcttcacag caatgcgacg tcgagccagc cgcgccggtc
     3241 gaagcgggcc tgattgccgg gccgcagcag cacggtggtg gtatccgatt cgatgatggc
     3301 ggggccggcg accgtctgat ccccagagag cgccgcgaag tcgaaaactg ggatttcgac
     3361 ccaaccgtcc aggaacacac gccggcttcg cgccggaacg tccccttgct tcggcgccga
     3421 acccgatgcc gccggggcag gcggcaagcg gccgatcacc gaaacgcggg cattcaccag
     3481 caccacatcc tggtcccgca acgcgtaggt gtacagcgct tcatgcgcct ggtggaacgc
     3541 cgcctcgatc gccgccacca cggacggcgc gtgcaggtcg acattgtcca gcggcactgg
     3601 aatttcgaac acctgctcgc cgtagcgcat gtcggcggcg cgccgcaccg cgatctcgcc
     3661 gtcgaaccaa gtcagccgcg cccgcccctc ctgctccatc gccgcgaagg cggcacgcag
     3721 cgcgtccggg tcgatgccgc gcgtctgcga ctggctgcgg gtcagctcga tccgcaggtc
     3781 ggtgttcagc atgccccagg ccgacagcac cgacgcggcc accggcacca ccacccggcc
     3841 aatgcccaac tccgccgcca ccgcggtgac atgcagcccg gcggcgccgc cgaaggcgag
     3901 cagcgtgtag ccgcgcggat cgacgccccg ccggaccgtg gcgacgcgca cgccatcggc
     3961 catccgggaa ttgaccaggc gatgaatgcc ggatgcggcc tccggcacgc cgatcccgag
     4021 cggctgcgcc acggcctgga tcgcccgggt ggccgcagcg aggtcaagct gccgccgccc
     4081 gcccaggaaa ttgccgggat cgagatagcc cagcaccagg ttggcgtcgg tcacggtggc
     4141 ggcggtgccg c
//
LOCUS       DASSZH010000237         3456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1556595, whole genome shotgun sequence.
ACCESSION   DASSZH010000237 DASSZH010000000
VERSION     DASSZH010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1556595"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VFN42_05085"
     CDS             <1..485
                     /locus_tag="VFN42_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8996023.1"
                     /translation="NNAGGSRVLPIDAPEEKWVEAMTLNFTRQRQLAHALLPRMIERK
                     WGRIINITGKSEPEHLNVAFAAKAAMHAWAKGLSREIGQHGITVNSIPPGRIMSEQIR
                     RNYPEDYRKHFAETEIPVRYWGEPEDLAVMAVWLASPLARYITGAVIPVDGGLRRYQF
                     "
     gene            503..1300
                     /locus_tag="VFN42_05090"
     CDS             503..1300
                     /locus_tag="VFN42_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2272 domain-containing protein"
                     /protein_id="HET8996024.1"
                     /translation="MRRGLALAGMLALAACARTGGADLHVPPFARVPYQPFSRTAVVA
                     IAMREWRLFGSPIDDAPPADRPPPLPQDKPEREAGLWQRVGEYWWEGMPPGDRESRWT
                     GKHNAEGVVFPASQDWEYAWSAAFIDYVMRIAGAGDRFPYAADHARYIDIAKDMALGR
                     ASGWLITAERPDAYAPQPGDLICTGRGTSAGLTYDDLPAAVFPAHCDIVVDTSVPGAI
                     SVIGGNVDDAVTMKHVPVTPDGKLADPNGAVLDTRYPWMVVIRLLAG"
     gene            complement(1305..2885)
                     /locus_tag="VFN42_05095"
     CDS             complement(1305..2885)
                     /locus_tag="VFN42_05095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8996025.1"
                     /translation="MRQELEQARVSLEDAQSELERMREECHGLLRAAAAQRAARARAE
                     VAAHRAQQNTASVLTAAARAGSDPQAESEELRISLEETANLAEELRCTNEALSEANAA
                     LDSRVAERTAALDRANAKLEALNAELQQRVEAEAAARAAAQAELFKLQKLEAVGQLTG
                     GIAHDFNNLLTVIISGLQLLGQVKDEAHRARVVRRTEEAAWRGAHLTQRLLAFARRQA
                     LSPERLDLLRHVDSIRDLLTHGLRENIQVHTDIDPALWPIEVDVAALELSLLNLAVNA
                     RDAMPQGGRLLLAARNAPLAVEPAERFSVAPGDYVEVSVADTGTGMPKELLDKVFEPF
                     FTTKSEGKGTGLGLAQVYGFVTQSGGTAWVESAPNQGTAVRLLLPRSTRPAPERTEEA
                     PPPPAPRQSSRLKVLVVEDDDNVAALVLDMLVQLGHSGTRVGTVASAMAVLTGAEQID
                     LVFSDVLLPGGGSGLDLARDMARRQIGVPMILTSGYGGGMTQRLAAANLPFLRKPYRM
                     DSLRQAIDEALARVPAAG"
     gene            complement(3090..>3456)
                     /locus_tag="VFN42_05100"
     CDS             complement(3090..>3456)
                     /locus_tag="VFN42_05100"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET8996026.1"
                     /translation="MRRRQPDASAEMLQRERDKALRAPVIVVVAAHVQRGHKIPEVEQ
                     VASAAAAAQTIMLAANAQGFGAMWKTGAPAYDASVKQALGLDPEDAVLGFLYLGRQVG
                     AGSPAARPAADAHVSVWQG"
BASE COUNT          505 a         1248 c         1169 g          534 t
ORIGIN      
        1 tgaacaacgc cggcggcagc cgcgtgctgc cgatcgacgc accggaggag aagtgggtgg
       61 aggcgatgac gctcaacttc acccgtcagc ggcaactggc gcacgcgctg ctgccgcgga
      121 tgatcgagcg caaatggggc cgcatcatca acatcaccgg caagagcgaa ccggagcacc
      181 tgaacgtcgc cttcgccgcc aaggccgcga tgcatgcctg ggccaagggg ttgtcgcggg
      241 agatcggcca acacggcatc accgtcaatt ccatcccgcc cgggcggatc atgagcgagc
      301 agatccgccg caactatccc gaggactacc gcaagcactt cgcggaaacc gagatcccgg
      361 tgcgctactg gggcgagccg gaggacctgg cggtgatggc ggtgtggctg gcctcaccgc
      421 tggcgcgcta catcaccggc gcggtgatcc cggtggatgg cggcttgcgg cgctatcagt
      481 tctgacggat cgcgtggcgc tgatgcggcg agggctggca ctggccggga tgctggcgct
      541 ggccgcctgc gcccgcaccg gcggggcgga cctgcacgtg ccgcccttcg cccgggtgcc
      601 ctaccagccc ttcagccgca ccgcggtcgt cgccatcgcc atgcgggaat ggcgcctgtt
      661 cggctcccct attgacgacg cgccgccggc tgaccgcccg ccgccgctgc cgcaggacaa
      721 gccggagcgc gaggccgggc tgtggcagcg ggtcggcgaa tactggtggg agggcatgcc
      781 gcccggcgac cgggaatcac gctggaccgg caagcacaat gccgagggcg tggtgtttcc
      841 cgccagccag gactgggaat acgcctggtc cgccgccttc atcgattacg tcatgcgcat
      901 cgccggcgcc ggcgaccgct tcccctatgc cgcggaccac gcccgctaca tcgacatcgc
      961 caaggacatg gcgctcggcc gcgcatccgg atggctgatc accgccgaac ggccggacgc
     1021 ctatgcgccg cagccgggag acctgatctg cacgggccgt ggtacatcgg cgggcctgac
     1081 ctatgacgat ctgcccgccg cggtgttccc ggcgcattgc gatatcgtgg tggacaccag
     1141 cgtgcccggg gcaatcagcg tgatcggcgg caatgtcgat gacgcggtca ccatgaaaca
     1201 tgtcccggtg accccggacg gcaaactggc cgacccgaac ggcgcggtgc tggacacccg
     1261 ctatccgtgg atggtggtga tccggctgct ggcgggctga gccgctagcc tgccgccggc
     1321 acccgcgcca gcgcctcatc gatcgcctgg cgcagcgagt ccatccggta gggtttgcgc
     1381 aggaacggca ggttcgccgc ggccagccgc tgcgtcatgc cgccgccata gccgctggtc
     1441 aggatcatcg gcacgccgat ctgccgccgt gccatgtcgc gcgccaggtc cagcccgctg
     1501 ccgccacccg gcagcagcac gtcggagaaa accagatcga tctgctccgc cccggtcagc
     1561 accgccatgg ccgaggcgac ggtacccacc cgggtgccgc tgtgtccaag ctggaccagc
     1621 atatccagca ccagcgcggc gacgttgtcg tcgtcctcca ccaccaggac cttcagcctg
     1681 ctgctctgcc gcggcgccgg cggtggcggt gcttcctccg tccgttccgg cgccggacgt
     1741 gtgctgcgcg gcagcagcag gcgcacggcg gtgccctggt tgggcgcact ctccacccag
     1801 gcggtaccac cggattgcgt cacgaagcca tagacctgcg caagcccgag gccggtcccc
     1861 ttgccctcgc tcttcgtggt gaagaacggc tcgaacacct tgtccagcag ctccttcggc
     1921 atgccggtac cggtatcggc gacgctgacc tccacgtagt cgcctggcgc gacgctgaac
     1981 cgttccgccg gctcgacggc cagcggcgcg ttgcgcgccg ccagcagcag gcggccaccc
     2041 tgcggcatgg catcccgtgc attcaccgcc aggttgagca gcgacagttc cagcgcggcg
     2101 acgtccacct cgatcggcca gagcgccgga tcgatatcgg tgtgcacctg gatgttctcg
     2161 cgcagcccgt gcgtcagcag atcgcggatg ctgtccacgt ggcgcagcag gtccagccgc
     2221 tccgggctca gtgcctggcg gcgcgcgaac gcgagcaggc gctgggtgag atgcgcgccg
     2281 cgccaggcgg cttcctcggt gcggcgcacc acgcgggcgc ggtgcgcctc gtccttcact
     2341 tggccgagca gctggaggcc gctgatgatg acggtgagca ggttgttgaa gtcgtgagcg
     2401 atgccgccgg tgagctgacc gaccgcttcc agtttctgca gcttgaacag ttcggcttgc
     2461 gcggcagccc gtgccgcggc ttccgcctcg acccgctgct gcagctccgc gttcagcgct
     2521 tccagcttgg cattggcgcg gtccagggcg gcggtgcgct ccgccacgcg gctgtccagc
     2581 gcggcgttcg cttcgctcag cgcctcgttg gtgcagcgca gctcctccgc cagattggcg
     2641 gtttcctcca gcgagatgcg caattcctcg ctttccgcct gcggatcgct gcccgcgcgg
     2701 gcagcggcgg tcagcacgct tgcggtgttc tgctgggcac gatgcgcggc cacctcggcc
     2761 cgcgcgcgtg ccgcgcgctg cgccgccgcg gcccgcagca agccgtggca ctcctcccgc
     2821 atcctctcca gttcgctttg cgcgtcctcc agcgacaccc gcgcctgctc cagttcctgg
     2881 cgcaaacgtg tgacgtcctc tggcgcgcag ttgtccgggt gagccattct gtgcagttac
     2941 gaaagactgt tagcgcgacc tgccaaggac gataacaccg gcgcgagcct gatgcgacga
     3001 cacgtatccg tgatcgcagg acgccaaagg gttcggggcc gagatggcag gccgcgcccc
     3061 cggggcagcc ggcacgtccg ccgaagaact cagccttgcc acaccgagac atgcgcgtcg
     3121 gcggccggcc gagcggccgg ggagcccgcg ccgacctgcc ggccgagata gaggaagccc
     3181 agcaccgcgt cttccgggtc gaggcccagc gcctgtttca ccgaggcatc gtatgccggc
     3241 gcaccggttt tccacattgc gccgaacccc tgcgcattgg cggccagcat gatggtctgc
     3301 gccgcggcgg cggcggaggc gacttgctcc acttccggga tcttgtggcc gcgctgcaca
     3361 tgcgccgcga ccaccacgat caccggcgca cgcagcgcct tgtcgcgctc acgctgcagc
     3421 atttccgccg aggcgtccgg ctgccgccgg cgcatc
//
LOCUS       DASSZH010000238         2872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_158906, whole genome shotgun sequence.
ACCESSION   DASSZH010000238 DASSZH010000000
VERSION     DASSZH010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158906"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VFN42_05105"
     CDS             <1..260
                     /locus_tag="VFN42_05105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8996027.1"
                     /translation="EFIAYRPDVLILDMIMPEKDGIDVLNEVLLSGIPTRIVLTSGYS
                     DAYLRLAQGVAQFHDTDQVSILKKPFRRQELVDMLQRITAD"
     gene            complement(272..1675)
                     /gene="thrC"
                     /locus_tag="VFN42_05110"
     CDS             complement(272..1675)
                     /gene="thrC"
                     /locus_tag="VFN42_05110"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET8996028.1"
                     /translation="MRYISTRGQAPVRDFAGVLLAGLAEDGGLYVPEAWPRLTPIDWR
                     ALRGVAYPALAARILQPFIGDAIPAETLQALCHEAYAGFGHPAVVPLIQLDTGLFVQE
                     LFHGPTLAFKDMAMQVLGRLFEHVLEAHDAQITIVGATSGDTGSAAIEACAGLERVKV
                     VILHPHNRTSEVQRRQMTTVHAPNIGNIAVEGTFDDCQDLVKAMFADAAFRQEMQLSA
                     VNSINWARIAAQVPYYAAAALALGAPDREVAFSVPTGNFGNVLAGWVARRMGLPVSRL
                     IVASNRNDILARFLASNDMSVSRVEPSLSPSMDIQVSSNFERALFELLDRDPVATSEA
                     MRNFRNTGRMPVPDLAWRRARSVFHGFRLDDEGTAAEIAALHASAGYIADPHTAIGIA
                     AARALPPPQAVATVALATAHPAKFPDAIEQAIGLRPNLPARLADLHGRAERFLVLPNE
                     LGAVQAGVRALTRRNLA"
     gene            1889..>2872
                     /locus_tag="VFN42_05115"
     CDS             1889..>2872
                     /locus_tag="VFN42_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996029.1"
                     /translation="MLWSVVALAVLGTVAAGAGTAVVLNRVGRTPRQWAPYLQRRAYE
                     HRPLIVNSVNLVAWWLNHAGRMAVSRPFPLPTSLGASPDRSGPVPPGRLRIVNTVQEA
                     LSAIGSAQPGDVIQLAPGKYAFPRTVIARPGSATAPITLRAARLGDVVVDATGPETFK
                     MYAPFWHLENLVMRGVCKYDGDCDNALHIVAGAQHLLIRNNHFEDFNAAIKINAESGK
                     FPDDGVIEGNSFTASHPRRAPNPITPIDLDVASGWDIRDNIITDFVRDWPDHGGATYG
                     AFAKGTGQANVFERNFVACQWKLRGWPGEQIGLSLGGGGMFPPSLDRDHGRS"
BASE COUNT          498 a          989 c          937 g          448 t
ORIGIN      
        1 aggaattcat cgcctaccgg ccggatgtgc tgatcctcga catgatcatg ccggaaaagg
       61 acggcatcga cgtgttgaat gaggtgctgc tgtccggcat ccccacccgg atcgtactga
      121 cctccggcta cagcgacgcc tacttgcgcc tggcgcaggg cgttgcacag ttccacgaca
      181 ccgatcaggt ttccatcctc aagaagccgt tccgccggca ggagctggtc gacatgctgc
      241 agcggatcac cgcagactag cggctggcgt cctaggccag attgcgccgc gtcagcgcac
      301 gcacgccagc ctgcaccgcc cccaactcgt tcggcagcac caggaaccgc tcggcccggc
      361 cgtgaagatc tgccagccga gccggcagat ttgggcgaag cccgatcgct tgctcgatcg
      421 cgtcggggaa cttcgccgga tgcgccgttg ccagcgccac ggtcgccacc gcctgcggcg
      481 gcggcagcgc acgcgccgcg gcaatgccga tggcagtgtg cggatcggcg atgtagccgg
      541 cggaggcatg cagcgcggcg atttccgcgg ccgtgccctc gtcatccagc cggaagccat
      601 gaaacaccga ccgcgcgcgc cgccacgcca gatccggcac cggcatgcgg ccagtgttgc
      661 ggaagttgcg catcgcctca ctggtggcga ccggatcgcg gtccagcagt tcgaacaatg
      721 cccgctcgaa attcgagctc acctggatat ccatgctcgg tgacaggctc ggctccacgc
      781 gcgacaccga catgtcgttg gacgccagaa aacgcgccag gatgtcgttg cggttcgacg
      841 cgacgatcag ccgcgacacc ggcagcccca tgcgccgcgc cacccagccc gccagcacgt
      901 tgccgaaatt tcccgtggga acgctgaacg ccacctcgcg gtccggagcg cccagcgcca
      961 gcgccgccgc agcgtagtac ggcacctggg cggcgatacg ggcccagttg atcgagttca
     1021 ctgccgacaa ctgcatctcc tgccggaacg cggcatcggc gaacatcgcc ttcaccagat
     1081 cctggcagtc gtcgaaggtg ccctccaccg cgatgttgcc gatgttcggc gcatgcaccg
     1141 tggtcatctg ccggcgctgc acttcgctgg tgcggttgtg cggatgcagg atcaccacct
     1201 tcacccgctc cagccccgca caggcttcga tcgccgcgga accggtgtcg ccggaggtcg
     1261 ccccgacgat ggttatctgc gcgtcgtgcg cctccagcac atgctcgaac agccgcccga
     1321 gcacctgcat cgccatgtcc ttgaaggcga gggtggggcc gtgaaacagt tcctgcacga
     1381 acaacccggt gtcgagctgg atcagcggca ccacggcggg atggccgaag ccggcatagg
     1441 cttcgtggca gagcgcttgc agcgtctccg ccgggattgc gtcgccgatg aagggctgca
     1501 ggatgcgcgc ggcgagggcg ggatatgcca cgccgcgcag cgcgcgccag tcgatcgggg
     1561 tcaggcgggg ccaggcttcc gggacataca gcccgccatc ctcggccaac ccggccagca
     1621 gcacgccggc gaaatcgcga acgggcgcct gcccgcgagt ggagatgtag cgcatgcccg
     1681 gcagattacg cggcgaacgg ccgactcgcg aggggggaac aaccagcccc gtcaggccga
     1741 cgtccggaat ccgaactggt ctttggccag gggtcgcctg tagtgtccgg ccatgcaaga
     1801 tcacgcgcgc ttgagctccg atttgtcccg ccggcccggc ggatccgagc ccggggctcg
     1861 gcgcaagcgg cgcagcggcg tcaaatgggt gctctggtcg gttgtcgcgc tggccgtgct
     1921 cggcaccgtc gccgccggcg ccggaacggc tgtggtactg aaccgggtcg ggcgcacgcc
     1981 gcgacagtgg gccccctatc tgcagcgccg tgcctatgag catcggccgc tgatcgtcaa
     2041 cagcgtcaat ctggtggcct ggtggctgaa ccatgcagga cgcatggcgg tgagccggcc
     2101 gtttccgctg ccgacctcac tcggcgcgtc accagaccgg tccggaccgg ttccgccagg
     2161 gcggctgagg atcgtcaaca ccgtccagga ggcactgagc gcgatcggca gcgcgcaacc
     2221 cggcgatgtc atccaacttg cgccggggaa gtacgcgttt ccccgcacgg tcatcgcccg
     2281 tcccggcagt gccactgcgc ccataacgtt gcgtgcggcg cgacttggcg atgtggtcgt
     2341 cgacgccacc gggccggaga ccttcaagat gtatgcgccg ttctggcatt tggagaacct
     2401 cgtcatgcgg ggagtctgca aatacgatgg cgactgcgac aacgcgctgc acatcgtggc
     2461 cggcgcccag catctgctga tccgcaacaa ccatttcgag gacttcaacg ccgcgatcaa
     2521 gatcaacgcc gaaagcggaa aattcccgga cgacggcgtc atcgaaggca acagcttcac
     2581 cgcgagccat ccgcgacggg cgcctaatcc gattacgccg attgatctgg atgtcgcgag
     2641 cggctgggac attcgcgaca acatcatcac cgacttcgtg cgcgattggc cggatcacgg
     2701 cggcgcgacc tatggcgcct tcgccaaggg caccggccag gcgaatgtgt tcgaacgcaa
     2761 cttcgtcgcc tgccaatgga agttgcgcgg ctggcctggc gaacagatcg gcctctcgct
     2821 cggtggcggc ggcatgttcc cgcccagcct agaccgcgac catggccgca gc
//
LOCUS       DASSZH010000239         6624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1079129, whole genome shotgun sequence.
ACCESSION   DASSZH010000239 DASSZH010000000
VERSION     DASSZH010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6624
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079129"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3303)
                     /locus_tag="VFN42_05120"
     CDS             complement(<1..3303)
                     /locus_tag="VFN42_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET8996030.1"
                     /translation="MNIMVQALSDANSTEAELRRVLGDQTLALGRDYADRGRVLRLTV
                     APDTGRIEAATQGNHPDPYHQSITLSRSPEGRLSIGSRCSCPMHSGCKHVAAVLLEAA
                     RRHKLLLLNPQGGLEYASLIQAPGRPTLVPAPTRPAAPPAGAPLPESVSAWLRDLEYA
                     SKTETEDFPPSQRQRLFYTLQLMPTVHGPRALRIQPMTVSVLKDGSLGATAQTFPLAR
                     YQNRQMPAFLRPSDRTIVQRLGAAQYGADDPRAEETLRMIIASGRARWGDLKGLEPRE
                     AAPRKSRLGWELQEDGSQRPVLHLAPPAVAMLIPAPWFVEPETGLLGPLEVDMPAGVA
                     SRLLRAPPVPLEHVERVRTELTRRVPHMALPQPDVPPPPEVLDAPLRPHLLLTTADMP
                     VPGYGRYLGGEVRPQPLAKLRYGYGPFLLAMGDRRPVMARERRLYRLTRDADAEAAAH
                     QRLQALGLTHFPGAVSSWHTHPWLNDLMLPSDEAWIAFLLRDAPELRRDGWTVEVADN
                     FPFRLAEAEGEWTAELAEGSGIDWLELHLGVMVDGQRIDLVPALLAMIESADRSGADL
                     AQMVDGHDGALPFLVPLRDGRLLSLPLGRIRPILLALWELFNGGGLDPSQGARITKLD
                     ATSVAALEEVAGVVWQGGEAVRALGRMLREAGDRIPQAKVPAVFRGALRPYQAQGVDW
                     LQFLRSAELGGILADDMGLGKTVQTLAHLLIEQDAGRLTKPALIVCPTSLVPNWTLEA
                     ERFAPSLKLLALHGPARKQHFGRIPEHDVVLTTYPLLARDHAVLTAQDWHIVVLDEAQ
                     TIKNPNAETTRQALQLKASQRLCLSGTPLQNHLGELWSLFDFLAPGFLGSARHFRTRF
                     RTPIEKHGDAPRQALLSKRVKPFLLRRTKEEVAVELPPKTEITEPVEMEAAQRGVYDA
                     IRLAMHSRVKAAIAAKGLARSGIIILDALLKLRQACCDPRLLKLDSVRKTKAGSAKLE
                     RLMELLTVMLEEGRRVLLFSQFTEMLALIERALHDAKIDYVMLTGDTIDRATPVRRFQ
                     AGEVPLFLISLKAGGVGLNLTAADTVIHYDPWWNPAVEDQATDRAHRIGQDKRVFVHR
                     LVTLGTIEEKMEL"
     gene            complement(3356..4432)
                     /locus_tag="VFN42_05125"
     CDS             complement(3356..4432)
                     /locus_tag="VFN42_05125"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET8996031.1"
                     /translation="MTRSYSTYLNLLRILAALAVFLSHAAARSFTNGTVWAPERMGHK
                     AVILFFVLSGYVIAYVAREREHTLASFAVSRAARIYSVALPALAVSFAVQLLLWHLGS
                     RVPVYQLTQPLKYLAVFTTFMGDSWFLREDAFANVPYWSLFYEVWYYAGFAAVFYLRG
                     WIRVVALVAVALLTGPRIWLLAPLWLLGCLVYRLHGRAGPGRLPARLLFAASLAAAWF
                     VLVIDPNSAINAWADRLSGGWMGAHLRYSQYVLGDLLTGGVVAVNLYAARYADLRFGW
                     AARPIAWGASFTFALYLLHYPLLQFYAALHLPVPALIAAVLGSALLLGQVTERQKDGL
                     RAWMMAALSVPAIGRIQARLPHSR"
     gene            4781..6070
                     /locus_tag="VFN42_05130"
                     /pseudo
     CDS             4781..6070
                     /locus_tag="VFN42_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631554.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
     gene            complement(6081..>6624)
                     /locus_tag="VFN42_05135"
     CDS             complement(6081..>6624)
                     /locus_tag="VFN42_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HET8996032.1"
                     /translation="WFTDCVVHPEHCPDYVQVDGGEGGTGAAPAPLIDYVGLPITEAL
                     PLVALARERHGLAERVRIVASGKLITPDKVAWALCMGADYVSAARGFMFSLGCIQAMK
                     CGTGRCPTGVTSAEPRLIAGLDPTDKAVRVARYAKQMREEVEIIAHACGLPDPSGFRP
                     QHVTEIERGVGGFRAPQGGG"
BASE COUNT         1100 a         2323 c         2272 g          929 t
ORIGIN      
        1 cagctccatc ttttcctcga tggtgcccag cgtcaccagc cggtgcacga acacccgctt
       61 gtcctggccg atgcggtggg cgcggtcggt ggcctggtcc tccaccgccg ggttccacca
      121 tggatcgtag tggatcaccg tatccgccgc ggtcaggttc aatcccactc cgccggcctt
      181 caggctgatc aggaacagcg gcacctcgcc cgcctggaag cgccgcaccg gcgtggcgcg
      241 gtcaatcgtg tcgccggtca gcatcacgta atcgatcttc gcgtcatgca gcgcgcgctc
      301 gatcagcgcc agcatctcgg tgaactggct gaacagcagc acgcgccgcc cctcctccag
      361 catcacggtc agcagctcca ttagccgctc cagcttggcg gagcccgcct tcgtcttgcg
      421 caccgaatcc agcttgagca ggcgcggatc gcagcaggcc tggcgcagct tcaacagcgc
      481 atccaggatg atgatgccgg atcgcgccag tcccttcgcg gcaatcgccg ctttcacgcg
      541 gctatgcatt gccagacgga tcgcatcgta cacgccgcgc tgggcagcct ccatttccac
      601 cggttcagtg atctcggtct tcggcggcag ttcgaccgcc acttcctcct tggtccggcg
      661 cagcaggaag ggcttcaccc gcttgctcag caatgcctgg cgcggcgcat cgccgtgctt
      721 ctcgatcggc gtgcggaagc gcgtgcggaa atgccgcgcg ctgcccagga agccgggggc
      781 caggaagtcg aacagcgacc acagctcgcc cagatggttc tgcagcggcg tgccggacag
      841 gcacagccgc tggctggcct tcagctgcaa cgcctggcga gtggtctccg cattcgggtt
      901 cttgatggtc tgagcttcgt ccagcaccac gatgtgccag tcctgcgcgg ttaacactgc
      961 atggtcgcgg gccagcagcg gataggtggt cagcaccacg tcgtgctccg ggatgcggcc
     1021 gaagtgctgc ttgcgtgccg ggccgtgcag cgccagcagc ttcagcgagg gagcgaaccg
     1081 ctccgcctcc agggtccagt tgggcaccag gctggtgggg cagacgatca gcgcgggctt
     1141 ggtcaatcgg ccggcgtcct gctcgatcag caggtgcgcc agggtctgca ccgtcttccc
     1201 caggcccatg tcgtcggcca ggatgccgcc cagttcggcg ctgcgcagga attgcagcca
     1261 gtccacgccc tgcgcctggt acggccgtag cgcgccgcgg aacaccgccg gcactttggc
     1321 ctgtgggatg cggtcgccgg cttcgcgcag catgcggccc agcgcccgca ccgcttcgcc
     1381 gccctgccag acaacgccgg ccacctcctc cagcgcggcg acgctggtgg cgtccagttt
     1441 cgtgatgcgc gccccttgcg agggatccag cccaccgccg ttgaacagct cccacagcgc
     1501 cagcaggatc gggcggatgc ggccgagcgg cagtgacagc aggcgaccgt cccgtagcgg
     1561 caccaggaag ggcagcgcgc cgtcatgccc atccaccatc tgcgccaggt cggcaccgga
     1621 gcggtccgcg ctctcgatca tcgccagcag ggcggggacg aggtcgatgc gctggccgtc
     1681 gaccatcacg cccagatgga gttccagcca gtcgataccg ctgccctcgg cgagttccgc
     1741 ggtccattcc ccctccgcct ccgcgaggcg gaaggggaag ttgtcggcga cctccaccgt
     1801 ccagccgtcc cggcgcagtt ccggcgcgtc gcgcaacagg aaggcgatcc atgcctcatc
     1861 ggacggcagc atcagatcgt tgagccacgg atgcgtgtgc caggacgaca cggcaccggg
     1921 gaaatgggtg aggccgagcg cctgcagccg ctggtgagcc gccgcctcgg cgtcggcatc
     1981 gcgggtgagg cggtatagcc ggcgctcgcg cgccatgacc gggcggcggt cacccatggc
     2041 cagcaggaag gggccgtagc cgtaacgcag cttcgccaaa ggctgcggcc gcacctcccc
     2101 gccgagatag cgcccgtacc cgggcaccgg catgtcggcg gtggtcagca gcagatgcgg
     2161 gcgcagcggt gcgtccagga cctccggcgg cggcggcacg tccggctgcg gcagagccat
     2221 atgcggcacc cggcgggtca gttccgtccg cacccgctcg acatgctcga gcggcaccgg
     2281 cggcgcacgc aacaatcgcg aggcaacgcc agctggcatg tccacctcca gcgggcccag
     2341 cagcccggtt tccggctcga cgaaccaggg ggccgggatc agcatggcga cggccggcgg
     2401 ggccaggtgc aggaccggac gctggctgcc gtcctcctgc agctcccagc ccaggcgcga
     2461 cttgcgtggt gccgcctccc gcggctccag ccctttcagg tcgccccagc gcgctcggcc
     2521 gctggcgatg atcatgcgca aggtctcttc cgcccggggg tcgtccgcgc cgtactgggc
     2581 ggcacccagg cgctggacga tggtccggtc ggacgggcgc aggaaggccg gcatctgtcg
     2641 gttctggtag cgggcaagcg gaaaggtttg tgctgtcgcc ccgaggctgc cgtccttcag
     2701 aacagacacg gtcatcggct gaatacgcag ggcccgtgga ccgtgcacag tcggcatcaa
     2761 ctggagcgtg tagaacaggc gctggcgctg gcttggcggg aaatcctcgg tttcagtctt
     2821 gctggcgtat tccaggtcgc gcagccaggc ggacacgctt tccgggagcg gggcgcccgc
     2881 gggcggcgca gccggacggg tcggcgccgg taccagagtc ggccggcccg gcgcctggat
     2941 caatgaggca tactcgagcc ccccctgcgg gttcagcagc agcagcttgt gccggcgagc
     3001 tgcctccagc agcaccgccg cgacgtgctt gcagccgctg tgcatcgggc aggagcatcg
     3061 gctgccgatt gagagccggc cctcggggct acgggagagg gtgatgctct gatggtaggg
     3121 gtcaggatgg ttgccctggg ttgcggcctc gatccgccca gtatcgggcg cgaccgtcag
     3181 gcgcagcacc cggccgcgat cggcatagtc gcgtcccaac gccagggttt ggtccccgag
     3241 aacgcggcgg agttccgcct cggtggagtt cgcatcggat agcgcctgaa ccatgatgtt
     3301 catggtgacg agcctaggtg gcttggtcga gtcggcagaa acagacaaac gccgatcagc
     3361 gcgaatgcgg caggcgggcc tggatccggc cgatggcggg gacggacagc gcggccatca
     3421 tccaggcgcg cagcccgtcc ttctggcgct ccgtcacctg cccgagcagc agggcactgc
     3481 ccagaaccgc ggcgatcagc gccggcaccg gcaggtgcag cgcggcgtag aattgcagca
     3541 gcgggtagtg cagcagatac agggcgaagg tgaaggacgc gccccaggcg atcggccgcg
     3601 ctgcccagcc gaaccgcaga tcggcgtagc gcgcggcata caggttcacc gccaccacgc
     3661 cgccggtcag caggtcgccg agcacgtatt gggaatagcg cagatgtgcc cccatccaac
     3721 cgccgctgag ccggtcggcc caggcattga tcgcgctgtt ggggtcgatc accagcacga
     3781 accacgccgc ggccaggctg gcggcgaaca gcagccgcgc cggcaagcgg cccggacccg
     3841 cccgcccgtg cagccggtag accaggcagc cgagcagcca gagcggcgcc agcagccaga
     3901 tgcggggtcc ggtcagcaac gccaccgcga ccagcgccac caccctgatc cagccccgca
     3961 gatagaagac ggccgcgaag ccggcgtagt accagacctc gtagaacagc gaccaatacg
     4021 ggacgttggc gaacgcgtcc tcgcgcagga accaggagtc gcccatgaag gtggtgaaca
     4081 ccgccagata tttcagcggc tgggtgagct ggtacaccgg aacgcggctg cccaggtgcc
     4141 acagcagcag ctgcaccgcg aagctgaccg ccagcgccgg cagcgccacc gagtagatcc
     4201 gcgccgctcg gctgaccgcg aagctggcca gcgtatgctc ccgttcccgc gcgacatagg
     4261 cgatgacata gccggacagc acgaagaaca agatgaccgc cttgtgcccc atgcgctcgg
     4321 gtgcccagac agtgccgttg gtgaaggaac gcgccgcagc gtgggagagg aacaccgcca
     4381 gggctgccag gatgcgcagc aggttgaggt aggtggaata ggaacgggtc acgggacggc
     4441 gccgcaattg gtgcaggtgc gagactaacc cggcggatgg cgtagggcca gatttggatg
     4501 cagccaatgc gtccagcggc ggccagcgat caggggcgcg atgcaaaccc gacagcacgg
     4561 cgcgttcgag gaatacacgc tggaggaaat cgaccgtgag gtcgcggcca tgaaacagct
     4621 cggcgtctga accccgcatg ggcattgtga tcgcagatac ggcgccgctg cgccagcacc
     4681 aacaccctat gcgtcggccc ccctgttgca ccgcacgcac aagccttaca caccaccacc
     4741 ggcggcccgc atccgggccg atagaccgga gaccgacgat atggccaacg tgacggtgat
     4801 cggcgcccag tggggcgacg agggcaaggg caaggtggtg gactggctcg ccagccgagc
     4861 cgacatcgtc gtgcgcttcc agggcggcca taacgccggc catacgctgg tggtcggcaa
     4921 ccagacctac aagctctccc tgctcccctc cggcctggtg cgcggcaagc tcggcatcat
     4981 cggcaacggc gtggtggtcg atcccgaggc gctgctggcc gagatcgaca aggtgcgcgc
     5041 ccagggcatc acggtcaccc cggagacgct gcgggtggcg gagaacgcca tcctgatcct
     5101 gccgctgcac ccggcgatcg accgtgcccg cgaggcggcg cgcggcgccc ggcagatcgg
     5161 caccaccggc cgcggcatcg gcccggccta tgaagacaag gtggccagac gcgccatccg
     5221 cgtcgccgac ctgacagagc cagagacggt ggccgccaag ctcgacgagc tgctgctgca
     5281 ccacaacacg ctgctggccg ggctgggcgc gcccaccttc gagaagcagg cgctgctcga
     5341 ccggctgatg gaactcgccc cgaagatcct gcccttcgcc gaaccggtct gggaacgcct
     5401 ggacgaagcg cgccgcgcgg gtcggcgcat cctgttcgag ggcgcccagg cggtgatgct
     5461 ggacgtcgat cacggcacct agcccttcgt cacctcctcc aacacggtgg cggcgacggc
     5521 cgcggccggc agcggcatgg gaccgggcgc ggtcggcttc gtgctgggca tcgccaaggc
     5581 ctacagcacg cgggtcggct ccggcccctt ccccaccgaa ctcaccgacg ccaccggcgc
     5641 cctgctcggc gaccgcgggg cggagttcgg cacggtcacc gggcggcggc ggcgctgcgg
     5701 ctggttcgac gccgcgctgg tgcgccaggc ggtgaaggtg ggcggcatcc agggcttggt
     5761 gctgaccaag ctggacgtgc tggacgggct gccggagctg aaaatcaatg tcggctaccg
     5821 tatcaacggg cagttggtga accacctgcc cgccgcgccc ggcgcgcagg cggccgccca
     5881 accggaatac gagacgatgc ccggctggac cggctccacc cgcggtgccc gctcctgggc
     5941 cgacctgccg gcccaggcgg tgaagtatgt tcgccgaatc gaggagctga ccgaagcccc
     6001 ggttaccctg gtctccacca gcccggagcg cgacgacacg atcctggtga aggacccgtt
     6061 cgaggggtag gcagcgggat tcaacctccg ccctgaggcg cccggaatcc accgacgccg
     6121 cgctcgattt ccgtgacatg ctgcgggcgg aaaccggatg ggtccggcag cccgcaggca
     6181 tgcgcgatga tctccacctc ctcccgcatc tgcttcgcgt agcgcgccac gcgcaccgcc
     6241 ttgtcggtcg ggtccaggcc ggcgatcagc cggggctccg cggaggtcac gcccgtcggg
     6301 caacgcccag taccgcactt catcgcctgg atgcagccca ggctgaacat aaagccgcgg
     6361 gccgcggaca cgtagtcggc ccccatgcac agcgcccacg ccaccttgtc cggcgtgatc
     6421 agcttgccgg aggcaacgat gcgaacgcgt tcagcaagcc cgtgccgttc gcgcgccagc
     6481 gccaccagcg gcaatgcctc cgtgatcggc aggccgacat agtcgatcag cggcgcaggc
     6541 gccgcgcccg tgccgccctc cccgccatcc acctgcacgt agtcggggca gtgctcggga
     6601 tgcacgacgc agtcggtgaa ccac
//
LOCUS       DASSZH010000240         2362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1301080, whole genome shotgun sequence.
ACCESSION   DASSZH010000240 DASSZH010000000
VERSION     DASSZH010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301080"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VFN42_05140"
     CDS             <1..435
                     /locus_tag="VFN42_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET8996033.1"
                     /translation="LACDFRLASESVQYALPEQRLGQIPGSGGSARLQKIVGITRTKD
                     IVMRSRRINAKQALDWGIATEVVADGELESATDALVKELVGFAPLAQRTAKKLLNDSE
                     DASLSIAIELEGHCYSRLRSSDDFREGVAAFHEKRHPNFRGS"
     gene            complement(558..1253)
                     /locus_tag="VFN42_05145"
     CDS             complement(558..1253)
                     /locus_tag="VFN42_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HET8996034.1"
                     /translation="MPTRNVDWPQAWRVIASRYPPINLFERLIADPAVWDALIALEQL
                     TNPRVRDELGDIALVAPDERVSGPGASYVMAAFTHINPKGSRFSDGSFGVYYAAATLE
                     TAIAETVFHFEAFARDAGDPARSEDMRVLVGTVAAPFEDIAALPEAEQRALLDPDSYS
                     ASQAYAARLREAGANGVVYPSVRHADGQCLGVFRPRAVELPHQERHLKYRWNGERVDR
                     YFDYARDEWITLG"
     gene            complement(1257..1655)
                     /locus_tag="VFN42_05150"
     CDS             complement(1257..1655)
                     /locus_tag="VFN42_05150"
                     /inference="COORDINATES: protein motif:HMM:NF021255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbcA/ParS/Xre antitoxin family protein"
                     /protein_id="HET8996035.1"
                     /translation="MLQSHERQRPDAPAIDPAAALRGFFAIADAWALTADDTRTLLGR
                     PAERTFYAWRAGNAVRVPMDTLRRIGYVAGIYKALELLYSDPHLADAWIKRPNRAFGG
                     QAPLQRMRAGDVTDLAAVRSYLDAARAPWS"
     gene            complement(2137..>2362)
                     /gene="fumC"
                     /locus_tag="VFN42_05155"
     CDS             complement(2137..>2362)
                     /gene="fumC"
                     /locus_tag="VFN42_05155"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003630812.1"
                     /note="class II family (does not require metal);
                     tetrameric enzyme; fumarase C; reversibly converts
                     (S)-malate to fumarate and water; functions in the TCA
                     cycle; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HET8996036.1"
                     /translation="KRIAEYVARSLMLVTALAPVIGYDKASQIAHHAAEHDLTLKQAA
                     LQLGLVDEQTFDRVVDPARMVTPYVAGAPG"
BASE COUNT          384 a          762 c          835 g          381 t
ORIGIN      
        1 ctggcctgcg atttccgact ggcgtcggag agcgtgcagt acgcgctgcc ggagcagagg
       61 ctgggacaaa ttcccggctc gggcggatcg gcccggctgc agaagatcgt cggcatcacg
      121 cgcaccaagg acatcgtgat gcgctcgcgc cggataaacg cgaagcaggc gctggattgg
      181 ggcatcgcaa ccgaggtggt cgcggatggc gagctggagt cggcgaccga tgcgctggtg
      241 aaggaactgg tgggtttcgc gccgctggcg cagcgcacgg cgaagaagct gctgaacgac
      301 agcgaggatg cgtcgctttc catcgccatt gaactggagg ggcactgcta cagccggctg
      361 cgctcctccg acgatttccg cgagggcgtg gcggcgttcc acgaaaagcg ccacccgaac
      421 ttccgcggca gctgatcctc cctgttgtgt catggccgga cgtggtccgg ccacccacgt
      481 ctttgcggcg gtcgtcctga caagacgtgg gtgcccgggc caagcccggg catgacactg
      541 ggggaggccg agccctccta ccccagcgtg atccactcat cgcgcgcgta gtcgaagtag
      601 cggtccaccc gttcgccatt ccagcggtat ttcagatgcc gctcctgatg cggcagttcg
      661 actgcgcggg gccggaacac gccgaggcac tggccatcgg catgccgcac gctgggatac
      721 accacaccgt tcgccccggc ctcgcgcagc cgcgccgcat aggcctgaga tgccgaataa
      781 gagtccgggt ccagcagcgc gcgctgctct gcttccggca gcgcggcgat gtcctcgaag
      841 ggcgccgcga ccgtgcccac caggacgcgc atgtcctcgc tgcgcgctgg atcgccggcg
      901 tcgcgggcga aggcttcgaa gtggaacacc gtctcggcga tcgccgtctc cagcgtggcg
      961 gcggcgtaat agacaccgaa gctgccgtcg ctgaagcgcg agcctttggg attgatgtgg
     1021 gtgaaggcgg ccatcacgta actggcgccg gggccgctga cccgttcgtc cggggcaacc
     1081 agggcgatgt cgccaagctc gtcgcggacc cttggattgg taagctgctc cagggcaatc
     1141 agcgcgtccc agacggcggg atcggcgatc agccgctcaa acaggttgat cggcgggtag
     1201 cgggaggcga tgacgcgcca ggcctggggc cagtccacgt tgcgggtcgg cacgggtcag
     1261 ctccacggcg cccgggccgc atccaggtaa ctgcggaccg ccgcgagatc ggtgacgtcg
     1321 ccggcgcgca tgcgttgcaa gggcgcttgg ccgccgaagg cgcggttcgg gcgtttgatc
     1381 cacgcgtcgg cgagatgcgg gtcggaatag agcagctcga gcgccttgta gatgccggcc
     1441 acgtagccga tgcggcgcag cgtatccatc ggcacccgca ccgcatttcc cgcgcgccag
     1501 gcatagaacg tccgctcggc tggccggccg agcagggtgc gggtgtcgtc ggcggtcagg
     1561 gcccaggcat cggcgatggc gaagaagccg cgcagggccg cggcggggtc gatggccggg
     1621 gcgtctggtc gctgccgttc gtgggattgg agcatcatgg ctctccgttc cgcatgaaat
     1681 tagctgttaa ctgcgaattt gcaaggccca gcgctctgat tgttcttgat ttgtctgaac
     1741 tgcctgctta gggtgccacc agatttagaa acatataggg aacaggacca tgcgatgcga
     1801 ccggtgtggg gaaaccatgc aaccggagac ggtaatccgg ctccgccgca gcttcggccg
     1861 cgtgcgcgcg acccatcggc agggcgggta ttgcgccggt tgcggcgcca gcgcggcggt
     1921 agaccgcggc ccgcgcaccg gggcgatcgg agccggcggt ggatggaccc ggttggcgct
     1981 gcgcgccaca gccgccttcc tcccggcggc agccggtccg cattgacacc aggacgggct
     2041 gtgtcgcgaa acctgctgcg gcaagctggc gcattcgccc tacccgaggg agactgccgc
     2101 gcgacgaaag acggcaggtt cgccggcgtg gcggcctcag cccggcgccc cggcgacata
     2161 aggcgtcacc atcctggcag gatcgaccac ccggtcgaat gtctgctcgt ccaccagccc
     2221 gagctgcaat gccgcctgct tcagggtcag atcgtgctcc gcggcatggt gggcgatctg
     2281 cgacgccttg tcgtagccga ttaccggtgc cagagccgtc accagcatca gtgaacgcgc
     2341 cacgtactcg gcgattcttt tg
//
LOCUS       DASSZH010000241         5038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1011297, whole genome shotgun sequence.
ACCESSION   DASSZH010000241 DASSZH010000000
VERSION     DASSZH010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011297"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..2787
                     /locus_tag="VFN42_05160"
     CDS             127..2787
                     /locus_tag="VFN42_05160"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HET8996037.1"
                     /translation="MAADPGTAFHTLDVAAVAERLGTHPAHGLSAADVVERQSRYGPN
                     ALREGQRRSKLRMLAAQFADFMILLLVAAAVVSGVIGDIEDTLVILAIVVLNAAIGFA
                     QELRAERAMAALKRMAPQQAVVLRDGAPHPVAAETLVPGDVVLLEAGNAVPADLRLTE
                     AVALRCGEAALTGESLPVEKTTAALPQAELPLGDRSNLAFKGTVVLYGRGRGIVTAIG
                     MATELGRIARLLDSAEAPRTPLQQRLTVFGRQIAIVALAICALIFAMGLLRGEPPLLM
                     LLTALSLAVAAIPEALPAVVTVLLALGAGRMARAQALIRRLPAVETLGSVTTICSDKT
                     GTLTRNEMRATEAFVGGRRIALDGLDPAGTPDDALLRGMALCNDVAAAEDGVLGDPTE
                     VALWRAAADLGLDKAALEHQAPRVFDVPFDSERKRMTTLHSAPDGLIAYVKGAPETVL
                     PRCRSIVTQAGDQLIDLPAVTGTAEQMAADGLRVLAVARRRWDGPVDAPSAEALERDL
                     VLLGLVGLLDPPRPETRQAVATCREAGITPVMITGDHPATARAIARRIGVLDDTASVL
                     TGRDLAALSDADLQQRVQSIAVYARVDPAQKIRIVTALQSAGEIVAMTGDGVNDAPAL
                     ARADIGVAMGRIGTDVARESASLVLLDDNFATIVAAVREGRRIYDNIRKFIRFVVTCN
                     AAEIWTIFLAPFLGLPVPLLPIQILWINLVTDGLPGLALAAEPAEPGVMQRPPRPPQE
                     GLFAGGMLRHVLWVGLLMAALTLGTQAYAIRSGMPHWRSMVFTVLTLVQMWQVMAIRS
                     DRESLFTQGLRSNTPLLGAVVLTFVLQLAVLYVPALNAVFDTSPLTAGELTACIAVSG
                     LAFVAIELHKWVGRKRRGADARAAPPSEPA"
     gene            complement(2942..4633)
                     /locus_tag="VFN42_05165"
     CDS             complement(2942..4633)
                     /locus_tag="VFN42_05165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HET8996038.1"
                     /translation="MYYLALAADYDGTIAKHGLVSDATVQALQALKQTGRRLVLVTGR
                     ELTDLRHVFPGMTMFDRIVGENGAVIYNPATSEERALAPPPPPALVHRLIERGVQPIS
                     VGHAIVATWQPHDVTALEVIRELGLELHIVFNKGAVMILPTHVNKAFGLRMALEELDI
                     SPRNVVAVGDAENDHAFLHACGCAAAVANALPALRDTADITLQGDHGHGVAELAQMIA
                     ERDGGIVPPSRRGILAGKDRAGEPVYLEPESLVLVAGVSGAGKSCFAKLLTERMSQGG
                     FEFCVIDPEGDYLGLRNAVTTGSSTEQPVTEDALRLLLRANINVAVNVLALAVPQRRT
                     LLAAIFKCVGKLRAQSGRPHWLIVDEAHQLLPPGEPHLRVAKPEQFAGTILLTVDPTG
                     LAPEVLHRVDVVLVLGQAAPDVIAGLAARLNVRPPDGRPLPATDEMVWWQPRSRAPLR
                     VLRRDKPCQAHNRHTGKYAIGDVGEQASFYFRAPNSHLRAGNLSEFLKIAGSIPESVW
                     MHHLRARDFSAWFRNVIRDDELADSAAVVEADHAIGFIESRARIGSAICERYILA"
     gene            complement(4812..>5038)
                     /locus_tag="VFN42_05170"
     CDS             complement(4812..>5038)
                     /locus_tag="VFN42_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996039.1"
                     /translation="FIRQVNAIALRRRILLLADLLQTGRHLMLIPELARLRTEAAFIA
                     FLDAPSVAHFRELSAIELDLRRIAVEFEQR"
BASE COUNT          831 a         1648 c         1724 g          835 t
ORIGIN      
        1 tcatgtccgg catcgtcatc gtcggactgg tgtatcgccc gcggacccgg gtgttccgta
       61 ccgtcggctg gaccagcctg gcgctgttca cgatctacct gctgaactcc tacacgctgt
      121 atctgcatgg cagctgaccc aggcaccgca ttccacacgc tcgatgtggc ggccgtcgcc
      181 gaacggctgg gcacccatcc ggcgcacggc ctgtccgccg ccgacgtggt ggagcggcag
      241 tcccgctacg gaccgaacgc gctgcgcgag ggccagcgcc gcagcaagct gcgcatgctc
      301 gccgcgcagt tcgccgactt catgatcctg ttgctggttg cggcggcggt ggtgtcgggc
      361 gtgatcggcg acatcgagga cacgctggtg atcctcgcca tcgtcgtgct caacgccgcg
      421 atcggcttcg cgcaggagct gcgcgccgaa cgggcgatgg cggcgctgaa acgaatggcg
      481 ccgcagcagg ccgtggtgct gcgcgacggc gccccgcatc cggttgccgc cgagacgctg
      541 gtgcccggcg acgtggtact gctggaggcg ggcaatgcag tgcccgccga cctgcgcctg
      601 accgaagcgg tggcgctgcg ctgtggcgag gcggcgctga ccggcgaatc cctgccggtg
      661 gaaaagacca cggccgcgct gccgcaagcc gagctgccgc tgggcgaccg tagcaatctg
      721 gcgttcaagg gcacggtggt gctgtacggc cgcggacgcg gcatcgtcac cgccatcggc
      781 atggcgacgg aactcggccg tatcgccaga ctgctggact ccgcggaggc cccgcgcact
      841 ccgctgcagc agcggctgac cgtgttcggc cggcagatcg ccatcgtcgc cctggcgatc
      901 tgcgcactga tcttcgccat ggggctgctg cgcggcgaac cgccgctgct gatgctgctg
      961 accgcgctca gcctggcggt cgcggccata cccgaggcgc tgccggcggt ggtgacggtg
     1021 ctgctggccc tcggtgccgg ccgcatggcc cgtgcccagg cgctgatccg ccgcctgcca
     1081 gcggtggaga cgctcggctc ggtcaccacg atctgctccg acaagaccgg aacgctgacg
     1141 cgcaacgaga tgcgcgcgac cgaggcattc gtcggcggcc ggcgcatcgc gctggatggg
     1201 ctcgatcccg ctggtacccc ggatgatgcg ctgctgcgcg ggatggctct gtgcaacgat
     1261 gtcgcggcgg cagaggacgg cgtgctgggc gacccgaccg aggtcgcgtt gtggcgcgct
     1321 gcggccgatc tcggcctcga caaggcggcg ctggaacacc aagcgccgcg cgtgttcgac
     1381 gtgccgttcg attccgaacg caagcggatg acgacgctgc acagcgcacc ggatgggttg
     1441 atagcctatg tcaaaggcgc accggagacc gtgctgccac gctgccgcag catcgtcacg
     1501 caggcgggcg accagctgat cgacctgccg gcggtcaccg ggacagccga gcagatggca
     1561 gcggacggat tgcgcgtgct ggctgtggca cggcggcgtt gggacggccc ggtcgacgcg
     1621 cccagcgccg aggcgttgga acgcgacctg gtcctgctcg gcctggtcgg cctgcttgac
     1681 ccgccccgtc cggagacgcg gcaggcagtg gccacctgcc gggaggccgg catcaccccg
     1741 gtgatgatca ccggcgatca cccggcgacg gcgcgcgcca tcgcccggcg gatcggcgtg
     1801 ctggacgaca ccgccagtgt gctgaccggc cgggacctgg cggcgctatc cgacgcggac
     1861 ctgcaacagc gcgtgcagtc gatcgccgtc tacgcccgtg tcgatccggc gcagaagatc
     1921 cgcatcgtca cggcactaca gtccgccggc gagatcgtcg cgatgaccgg cgacggggtg
     1981 aacgacgcgc cggcgctggc gcgagctgat atcggcgtgg cgatgggccg catcggcacc
     2041 gacgtggcgc gggaatccgc cagcctggtg ctgctggacg acaatttcgc caccatcgtc
     2101 gctgcggtgc gcgagggccg gcgcatctac gacaatatcc gcaagttcat ccgcttcgtg
     2161 gtgacctgca acgcggcgga gatctggacg atcttcctgg cgccgttcct cggcctgccg
     2221 gtgccgctgc tgccgatcca gatcctgtgg atcaacctgg tcaccgacgg cctgccgggc
     2281 ctggcgctgg cggcggagcc ggcggaaccc ggggtgatgc agcggccgcc gcgtccgccg
     2341 caggaaggcc tgttcgccgg tggcatgctg cgccacgtac tgtgggtcgg cctgctgatg
     2401 gcggcattga cgctgggcac ccaggcctac gccattcgaa gcggcatgcc gcactggcgc
     2461 agcatggtgt tcaccgtgct gacgctggtg cagatgtggc aggtgatggc gatccgctcg
     2521 gaccgggagt cgctgttcac ccaggggctg aggtcgaaca cgccactgct gggcgcggtc
     2581 gtactgacct tcgtgctgca actggcggtg ctctatgtgc cggcgctgaa cgcggtattt
     2641 gatacgtcgc ccctgaccgc aggcgagttg accgcgtgca tagcggtatc aggcctggcg
     2701 ttcgtggcga tcgagctgca caagtgggtc ggtcgcaagc gccgcggagc tgatgcacgg
     2761 gctgctccac cttcagagcc cgcgtaagag gatcgagagc agaactccag cgatccgagt
     2821 gtcttccagg gtctggcgcc ggtcgtgcac cacaaggatt tcgccactca ttgatgaggg
     2881 cgtccgtacc gaacagccga tgggcaatcg ccggcacttg cattggcgct caaatgctct
     2941 gtcatgcgag aatgtagcgt tcgcagatcg cgctcccgat gcgggcccgg ctttcgataa
     3001 agccgattgc atggtccgct tccacgacgg cggcgctgtc cgccagttca tcatcgcgga
     3061 tcacgttgcg gaaccaggcg gagaaatctc tggcacgcag atggtgcatc cataccgatt
     3121 cggggatact gccggcaatc ttcaggaatt cagacaggtt gccggcgcgc agatgcgagt
     3181 tgggagcgcg gaagtaaaag ctggcctgct caccaacatc gcctatcgca tatttgccgg
     3241 tgtgacggtt gtgcgcctgg cacggcttgt cacgccgcag cacacgcagc ggagctctgg
     3301 aacgaggctg ccaccacacc atctcgtctg tcgccggcag tggccgaccg tcgggcggcc
     3361 tgacattcag gcgagccgcc aacccggcaa tcacatcagg cgccgcctgg ccgagcacca
     3421 ggacaacatc gacacgatgg agcacttccg gcgcgaggcc agttgggtcc accgtgagca
     3481 aaatcgtgcc ggcgaactgc tctggcttcg ccaccctgag atgcggttcg ccgggcggca
     3541 gcaactggtg cgcctcatcg acgatcagcc aatgcggccg tcccgattgc gcacgcagct
     3601 tgccgacaca tttgaatatc gcggccagca gtgtgcgccg ctgcggcacc gccagtgcca
     3661 gaacgttgac ggccacgttg atgttggcgc gcagcagcag ccgcagagcg tcttctgtca
     3721 ccggttgctc ggtgctgctt ccggtggtga ctgcattgcg caggccaaga tagtcgccct
     3781 cgggatcgat gacgcagaac tcgaaaccgc cctggctcat gcgctcggtc agcagcttgg
     3841 caaaacatga tttgccggcg ccagagacgc cggcaacgag caccagtgac tccggttcga
     3901 gatacacggg ctcgccggcc cgatccttgc ctgcgaggat gccgcgccgg gagggcggaa
     3961 caatgccacc gtcacgctcg gcgatcatct gcgcgagttc ggcgacgccg tggccgtgat
     4021 cgccttgcag agtgatgtcg gctgtgtcac ggagcgccgg caaggcattg gccaccgccg
     4081 cggcgcagcc gcaggcatgc aggaacgcat ggtcgttctc ggcgtcgcca acagcaacga
     4141 cgttccgcgg cgatatgtcg agttcctcaa gcgccatgcg cagaccgaac gctttgttga
     4201 catgcgtcgg caggatcatc accgcgccct tgttgaagac gatgtgcagc tccaggccaa
     4261 gctcgcggat cacctccagc gcggtcacgt catgcggctg ccaggtggca acgatcgcat
     4321 gaccgaccga gatcggctgc accccgcgtt cgatcaggcg atggaccagg gctggcggtg
     4381 gcggcggcgc cagggcgcgc tcttccgatg tggctgggtt gtagatgacc gcgccgtttt
     4441 cgccgacgat gcgatcaaac atggtcatgc cggggaagac gtgccgcaga tcggtcaact
     4501 cgcggccggt gaccagcacg aggcgccggc cggtttgctt caatgcctgc aaggcctgga
     4561 cggtggcatc actcaccagc ccgtgcttgg caatggtccc atcgtagtcg gcagccaggg
     4621 caagatagta catgacggcc agcttcgaac agcgacgacc tccgccccca ggctctacgc
     4681 cttaatgatt tgtttacgcg gttcaggcca ggcggaacat tgacctggat caattatttg
     4741 ctcaggctgg ttgcaacccg aaggtgtata tgatcgggta gagtgcctgg cgaacgcgac
     4801 gcgtccgtcg gtcatctttg ttcgaattcc acggcgatcc gccgcagatc aagctcaatc
     4861 gccgaaagct cccggaagtg cgccaccgaa ggcgcgtcca gaaacgcgat gaaagcggcc
     4921 tccgtgcgaa ggcgcgccag ttcgggaatc agcatcaggt gccgaccggt ttgcagcagg
     4981 tcggcaagca gcaggatgcg ccggcggagt gcgatggcat tcacctggcg gatgaaac
//
LOCUS       DASSZH010000242         2818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_943328, whole genome shotgun sequence.
ACCESSION   DASSZH010000242 DASSZH010000000
VERSION     DASSZH010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943328"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1329
                     /locus_tag="VFN42_05175"
     CDS             <1..1329
                     /locus_tag="VFN42_05175"
                     /inference="COORDINATES: protein motif:HMM:NF036810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HET8996040.1"
                     /translation="FTRLAGVADLFLVHDRDIRTRVDDSILRVTQHPKLAKPVTTFIR
                     RARGYAPYPVQLPEPVRPVLACGAELKSTIALGRNAAVFLSQHIGDLTNPATLDAHAD
                     AASQMAQLLTGQPAAIACDLHPQCHSAALRSAALPVVRVQHHHAHMAACMAENGLRGR
                     TIGVILDGTGYGTDGTIWGGEFLLGDCAGFTRAAHLRRFRLPGGDAAVMQPFRVAVAL
                     LHDALGEAWQTLPLPFAERLGNGRAMLLQRMCEAGINAPITTSMGRLFDGVAALLGLC
                     PVIEYEAQAAIELEGLLQRDLHPAEPLPFVVSEQDGVLEIDHRPLVRAVADAIGHGGI
                     DGAAVSRRFHSSVVAMIVAMSARLAATAGTRSVVLSGGVFMNEFVLANVIVGLREAGL
                     EPHWHGKVPPNDGGISLGQVLVADARLGSAAVDGRSADSEETPPLRLPTG"
     gene            complement(1363..>2818)
                     /locus_tag="VFN42_05180"
     CDS             complement(1363..>2818)
                     /locus_tag="VFN42_05180"
                     /inference="COORDINATES: protein motif:HMM:NF024795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HET8996041.1"
                     /translation="PGNTGAVDGNVDFGNGVSASLLVGLALHELTHAMGRISDGPTTP
                     DIFDLFHFSSQGTILADGNAPGGPSAYFSVDGGTTEIVTYGTSSDTSDFFNGNGLEDP
                     FTEFYDFNTPQTLTALDKQQLDVLGFDTACFAEGTRLATLNGPMPVEALRPGMKVLTV
                     SGAVRPVRWIGHRRIDLSRHPAAEEVCPIRIRAGALEDGVPIRDLRVSPEHAMFLGGG
                     LVPARLLVNGSSIVKETECRLVVWYHVELDSHDLVLAEGAATETYLDTGNRGLFENSG
                     DPLILHPDFNPGQAMRVARSCAPFLDQPAQVEPIWRRLTERAAGLGFETVATLPSTAD
                     PDLAIRADGRLFRPVGVARGRHVFVLPPVHGEVRLVSLASPPNALRPWIEDRRGLGIA
                     VSQITVRSARGTRVLTADDPALSDGWFAAEFDADGPRRWTNGDAALPIKSGCPAVVDI
                     RCQPQPAYVLESKRRARIGVAPSVAGLSRARACL"
BASE COUNT          458 a          951 c          968 g          441 t
ORIGIN      
        1 ttcaccagac tcgccggtgt cgccgatctg ttcctggtgc atgaccgcga catccgcacc
       61 cgggtggacg attccatcct gcgcgtcacg cagcatccga agctggccaa accggtcacc
      121 accttcatcc gccgcgcgcg cggttatgcg ccgtacccgg tgcaactgcc ggaaccggtc
      181 cgcccggtgc tggcctgtgg cgcggagctg aaatccacca tcgcgctggg ccgcaacgcg
      241 gcggtgttcc tcagccagca tatcggcgac ctgaccaacc cggcgacgct ggacgcgcat
      301 gcggatgccg catcgcagat ggcgcaactg ctgaccgggc agcccgcggc gatcgcctgc
      361 gacctgcacc cgcaatgcca cagcgcggcg ctgcgcagtg ccgcgctgcc ggtcgtgcgc
      421 gtgcagcacc accacgccca catggccgcc tgcatggcgg agaacgggct gcgcggccgc
      481 acgatcggcg tgatcctgga cggcaccggc tacggcaccg acggcacgat ctggggtggc
      541 gagttcctgc tcggcgactg cgccggattc acccgggccg cgcatctgcg ccgcttccgc
      601 ctgcccggcg gcgacgcggc ggtgatgcag ccgttccgtg tcgccgtcgc cctgctgcac
      661 gacgcgctcg gcgaggcctg gcaaaccctg ccactgccct tcgccgagcg cctgggcaat
      721 ggcagggcga tgctgctgca gcgtatgtgc gaggccggga tcaacgcccc catcaccacc
      781 agcatgggcc ggctgttcga tggcgtggcg gcgttgctgg gcctctgccc ggtcatcgag
      841 tacgaggcgc aggcggcgat cgagctggag ggtctgttgc agcgcgacct gcatccggcc
      901 gaaccgctgc cgttcgtggt gagcgagcag gatggcgtgc tggagatcga tcaccggccg
      961 ctggtgcggg ccgtggcgga tgcgatcggc cacggcggga ttgatggtgc cgcggtcagc
     1021 cgccggttcc attccagcgt ggtggcgatg atcgtggcga tgtcggcgcg gctggcggca
     1081 accgccggga cgcggtcggt cgtgctcagc ggcggcgtgt tcatgaacga attcgtgctg
     1141 gccaatgtga tcgtgggtct gcgcgaggcc ggactggagc cgcactggca cggcaaggtc
     1201 ccgccgaatg acggcgggat atcgctcggc caggtgctgg tggcggacgc gcggcttggt
     1261 tcggctgctg ttgatggcag gagcgccgat tcggaggaga cgccgccgct gagactgccg
     1321 accggatagc ggtccgccag ccggccggtg aagggcggga ggtcagaggc aggcgcgggc
     1381 tctgctcagt ccagcgaccg acggcgccac accgatccgg gcgcgacgtt tcgactccag
     1441 cacataggcc ggctgtggct ggcagcgaat gtccacgacc gccggacacc cggactttat
     1501 cggtaaggcg gcatcgccgt tggtccagcg gcgcggccca tcggcatcga actcggccgc
     1561 gaaccagccg tcggataacg ccggatcgtc cgccgtcagc acgcgcgtgc cgcgtgcaga
     1621 gcgcaccgtg atttgcgaaa ccgcgatccc aaggcccctg cggtcctcga tccatggccg
     1681 caacgcgttc ggcggggatg caagcgacac cagacggacc tccccatgca ccggcggcag
     1741 cacgaagaca tgccggccgc gtgccacgcc caccggacgg aacagccggc cgtctgcgcg
     1801 aatggccaga tcgggatcgg cggtcgaggg aagcgtggcg accgtctcaa accccagccc
     1861 tgccgcccgc tcggttagcc gccgccagat gggttcgacc tgtgcaggct gatccagaaa
     1921 aggtgcgcag gagcgcgcca cgcgcatcgc ctgacctggg ttgaaatcag gatgcaggat
     1981 caacgggtcg ccgctgttct cgaacaggcc gcgattgccg gtgtccagat aggtttccgt
     2041 cgcagcgcct tccgccagca caaggtcgtg cgagtccagc tcgacatggt accagacgac
     2101 cagccggcat tccgtttcct tcacgatgga actgccgttc accagcagcc gagcgggaac
     2161 cagcccgccg ccaaggaaca tcgcgtgctc gggcgagacc cgcaggtcgc gtatcggcac
     2221 accatcctcc agcgcgccgg cgcgaatgcg gatggggcag acttcctcgg ctgccggatg
     2281 gcgtgacaaa tcgatgcggc ggtggccgat ccagcgcacc ggccgcaccg cgccggagac
     2341 cgtcagcacc ttcatgccgg gtcgcagcgc ctccaccggc atcggtccgt tgagggtggc
     2401 cagccgcgtt ccctcggcaa agcacgccgt gtcgaagccc agtacatcca gttgctgctt
     2461 gtcgagtgcg gtgagagtct gcggcgtatt gaagtcgtag aattccgtga agggatcttc
     2521 caatccgttg ccgttgaaga agtcggacgt gtcggagctg gtgccgtagg tgacgatttc
     2581 cgttgttccg ccatccacgg aaaaataggc cgagggtcca ccgggagcgt tcccatctgc
     2641 caggatcgtg ccttggctgc tgaaatgaaa cagatcgaag atatccggcg tggtcggccc
     2701 atccgaaatg cggcccatcg catgggtcaa ctcgtgcagc gccaggccaa ccagcaggct
     2761 tgcgctgacg ccgttgccaa aatcgacgtt gccgtccacg gcgcccgtat tgcccggc
//
LOCUS       DASSZH010000243         7077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1454781, whole genome shotgun sequence.
ACCESSION   DASSZH010000243 DASSZH010000000
VERSION     DASSZH010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7077
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454781"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(290..466)
                     /locus_tag="VFN42_05185"
     CDS             complement(290..466)
                     /locus_tag="VFN42_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996042.1"
                     /translation="MLREQAARARRLARLIPGDPAEREMEVLAAQLDAEADKLELEPA
                     TRQPVVSRLRSGAA"
     gene            946..1128
                     /locus_tag="VFN42_05190"
     CDS             946..1128
                     /locus_tag="VFN42_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996043.1"
                     /translation="MHDATPASLREAAAHARRHAKAVWPHAAGQQLMAYAEELEQQAA
                     ALEQAGCGGTTVDRVA"
     gene            complement(1499..1726)
                     /locus_tag="VFN42_05195"
     CDS             complement(1499..1726)
                     /locus_tag="VFN42_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996044.1"
                     /translation="MPSDKPASQLTDAELAKLVAERAYQAYKSGDHGTGDKFKEVAKM
                     IHADSAEGFLQQLGQGAGGLGKLFGGGDDAA"
     gene            complement(2236..3882)
                     /locus_tag="VFN42_05200"
     CDS             complement(2236..3882)
                     /locus_tag="VFN42_05200"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HET8996045.1"
                     /translation="MNAPLPRPSGFQYAPMFPLGPDRIAWKRLPVAGVRTATCDGQTV
                     LRIAPDALSELAFQAFRDVSHLLRPAHLAQLRAILDDPEASANDRFVALDLLKNANIA
                     AGGVLPMCQDTGTAIVFGKKGQRVWVDGDEEEALAWGVHRTYTETNLRDSQMAPLTMF
                     DEVNTGNNLPVQFDILAAPGEHHADEFHLMFVAKGGGSANKSFLFQETRAILSPERLL
                     KFIETKIKTLGTSACPPYHLAIVIGGTSAETTMKTVKLASTKWLDTLPTSGSKAGHAF
                     RDLEMEQQVLELTRHIGIGAQFGGKYFCHDVRVVRLPRHGASLPVGIGVSCSADRQIM
                     AKITPDGVFLEQLETDPAKYLPETTDEHLGGDAVAIDLNQPMEAIRKQLSAQPVKTRV
                     MLTGTMVVARDIAHAKLKERLDRGEGLPDYLKNHPVYYAGPAKTPTGYATGSFGPTTA
                     GRMDSYVDAFQAAGGSLVMLAKGNRSKAVKDACRKYGGFYLGSVGGPAARLARDCIRK
                     VEVLEYPELGMEAVWRIEVENFPAFIVMDDKGNDFYEGLE"
     gene            complement(3955..5508)
                     /locus_tag="VFN42_05205"
     CDS             complement(3955..5508)
                     /locus_tag="VFN42_05205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET8996046.1"
                     /translation="MARWDDGYVTDVVYTSNFHRETTPAWLSTTALLLGHRPPSLAAP
                     FRYADLGCGNGLTALVVAATSPQAEVWGFDFNPAHIETARHLAARAGLGNVRFVETAF
                     ADLAALPPDGLPEFDFMVSHGVASWIAPEHRRHMQAVIRQRLRPGGLAYLSYNVTAGW
                     SSMVPLRKLMRMLAEASPARADQAAVEALDMIDRLKAAGALFFANNPGLEPRLQDMRR
                     QDPRYIAHEFLNQDWHPLSFADIASEMAECKCSYIGSATLTENIDAASVPPEMVQILA
                     ESRDPVLRETLRDLASCQGFRRDLYRRGITPIPAGEHRAMLDAITIASTGQTVPEPIT
                     FNTAIGTISGRPETYQPIQRRLAEGPVSVGELQASDAFAGRPPVEVLQAISLMIAGGY
                     AHPLLPDGGSAAVRQSTRALNQAIAALNADGGELPQVALPLLGSAIPVDAVETLLLGE
                     LLAGKPAEPQGLVADLLSLLARAGRTLQRDGKPVTDAAEAGGLAADLVQQFLRQRLPL
                     LRSLGVLDG"
     gene            complement(5557..6390)
                     /locus_tag="VFN42_05210"
     CDS             complement(5557..6390)
                     /locus_tag="VFN42_05210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET8996047.1"
                     /translation="MVWAAFRESITDHMSLAAAGCGFYATLALFPAISILISVYGLVF
                     NRDDILGQIKVLGELLPPPAFALIEGRVHELVGQYYGHVSLGLVLGLLLTFWSASTGS
                     KSMLSAINVAYDVAEQRPMLRFQAVGLSITLAAVLVAGLTMAVLVFLPVVIAFVGLSA
                     YNSGLIRAASVIMLVGLFGSTVALLYRYGPSRTPPPHQPIAPGAIVATALWLLASTLL
                     SLYISRLGTFGATYGPLGAAIGVMLWFYVSAYAVLLGAELNAQIEARRAAAALENPPT
                     D"
     gene            6748..>7077
                     /locus_tag="VFN42_05215"
     CDS             6748..>7077
                     /locus_tag="VFN42_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003617003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HET8996048.1"
                     /translation="MTPKIARFLVQQQPATPCLVLDLERVEANFRALQHALPLARIYY
                     AVKANPAPQVLDRLVRLSSSFDAASIEEVRACLDAGARPDAISFGNTIKKGSAIRAAH
                     ERGVSLFA"
BASE COUNT         1105 a         2450 c         2402 g         1120 t
ORIGIN      
        1 cagcgcgccg ctcaatgcgt tccggcactt ggctggtccg gatcgcatct gcgtgcatcc
       61 gcgtgcgcct gcggcaaaaa tcttccttgg cggtgccacc agtgccgagg ccaagcggcg
      121 cgggcgcggg cttcatgcgg acgcgcgccg acgccgatca agcgcgccga tgaatgaccg
      181 gccctccccc gcagtcgcgg gccgttgcag gcgtatccag accgcgtggt tccacccgat
      241 caccctgcgc ttgcccctgg cttgccgggg cggccgaatt gatcgggcgc tatgccgccc
      301 cggaccggag gcgggagacc accggctgtc gcgtcgccgg ctcaagctcc agcttgtccg
      361 cctcggcatc caattgtgcg gccaggacct ccatttcccg ttccgccgga tcgccaggga
      421 tcagtctcgc cagccgccgg gcacgcgcgg cctgctcgcg cagcaccccc actctcgacc
      481 gaatgtcgcc tttcgcacca ttcatgtgcc ggcgaacgcg caaggggaga gccggtttca
      541 gcgaagcggc gctccgcaat cggcggtggt ggctggccag tcggccgttt ggcttggacc
      601 gtgcaggccg gtcccttcgg atccgctttg catcagggcg gcgaaccgcg cggcacatgg
      661 ccccggcagc gaccgcgaca agcccatttg tgatcggtcc aattgatcca gatcagccgc
      721 tcacgtgagc gtttcccaca aaggtgtttc cgacaaacag gaaagcctgt cgatgcatgc
      781 cgatgaacac gaacccggaa ggccggcccc ccaatcgggg cgcttccagc tcagcaacgt
      841 cttcggcacg ttgaccggcg aggttgccgt tgtggcccag ggacagccgc tgccgccggc
      901 gccgcgtggc taccgatggg tgctgcggcc gaagacggat tgctgatgca cgacgccaca
      961 cctgccagtt tgcgggaagc ggccgcacac gcacgccgcc atgccaaggc cgtgtggccg
     1021 catgcggcgg ggcaacaact gatggcctac gccgaggagt tggagcagca ggcagctgct
     1081 ctggaacaag ccggctgcgg cgggacaacc gttgatcgcg tggcctgact gactcggagc
     1141 ggcttggcga gcactatccg gcatcccccg cccgcgcggc ccatactggg ctgaaagccc
     1201 agtctcaccc tcggcggcga agccggacgc aaaccggcgt tcagccttgg cctgtgatct
     1261 ctgcccacca tttccgaacc accgggtaca cggctcgggg ccaatctcct cgccgcgcgc
     1321 ttcgccggct ttcattcgca cggagcaagc gtggagcacc acggtgggca cggagccaga
     1381 gcggcccgcc gccacggggc ggctccggct gctgtgtcca cggtgctgct ccgcgcctgc
     1441 tccatgcgaa acaaaaaaag cccggcaagg agcacagtcg gtcggcgttc ctaccgcgtt
     1501 acgccgcgtc gtccccacca ccgaacagct ttcccaatcc gccagcgccc tgcccaagct
     1561 gctgcaggaa gccttccgca ctgtcggcgt ggatcatctt ggcgacttcc ttgaatttgt
     1621 cgccggtgcc gtggtcgccg cttttgtagg cctggtaggc acgttcggcg accaatttgg
     1681 caagttcggc gtcggtcagt tgcgatgcgg gtttgtcgga cggcattgcg attctcctct
     1741 ggtctgccgc gaacgtcgcc cggcaaaccg ggttccgtat cgccgccgcg ccgcgctgcg
     1801 ccggccaccg caagcgagtc cgcatgccgc cggatgccgc catcgccacc gccaaccggc
     1861 gcacaacaaa atgcgcgtgt ccgaggcgaa caatccgcgg gatgttagcg gctcctaacg
     1921 cgcctcggcc gggaaagccg cgcaggcgga acaatcctgc cggcattccg tacgccgcat
     1981 ccggcggcga tcggaaaccc tgcatgcagc aatagcaccg agatcgcatc atgcgcccgg
     2041 ccgcccgcgt cccgacagtc cggccgctcg ggtgcgggtg cggcgggcaa ctgccgttcc
     2101 tacgccttct ccaaacacgc ggcagaggtg agaccgtccg gaccaccgag cgcaaggaaa
     2161 gctggacgtg tcgcacgagg gcgggcgctg acgccgggcc gtgggcggac atggtccgcc
     2221 gacgtgccga gtacgctatt ccagtccctc gtagaaatcg ttccccttgt cgtccatcac
     2281 gatgaacgcc gggaagttct ccacctcgat ccgccacacg gcctccatgc cgagttccgg
     2341 gtactccagc acctccacct tgcggatgca gtcccgcgcc agccgcgccg ccggaccgcc
     2401 gacgctgccc agatagaacc cgccatattt gcggcaggcg tccttcaccg ccttggatcg
     2461 gttgcccttc gccagcatca ccagcgagcc gcccgccgcc tggaacgcgt ccacgtagct
     2521 gtccatccgc ccggcagtgg tggggccgaa gctgccggtg gcatagccgg tcggggtctt
     2581 ggccgggccg gcatagtaca ccgggtggtt cttcaggtaa tcgggcaggc cctcgccgcg
     2641 gtccagccgc tccttcagct tggcatgcgc aatgtcgcgc gccaccacca tggtgccggt
     2701 cagcatcacc cgcgtcttca ccggctgcgc cgacagctgc ttgcggatcg cctccatcgg
     2761 ctggttcagg tcgatcgcca cggcatcgcc gcccagatgc tcatcggtgg tctcaggcag
     2821 gtatttcgcc gggtcggtct ccaactgctc caggaacacg ccgtccgggg tgatcttcgc
     2881 catgatctgc cggtccgcgc tgcacgacac gccgatgccc accggcagcg aggcaccgtg
     2941 ccgcggcagg cggaccaccc gcacgtcgtg acagaaatac ttgccgccga actgcgcgcc
     3001 gatgccgata tggcgcgtga gttccagcac ctgttgttcc atctccaggt cacggaacgc
     3061 gtgccccgcc ttgctgccgc tggtgggcag cgtgtccagc cacttggtgg aagccagctt
     3121 caccgtcttc atcgtcgtct cggccgacgt gccgccgatg acgatcgcca ggtggtacgg
     3181 cgggcaggcg gaggtgccga gcgtcttgat cttggtttcg atgaatttga gcagccgctc
     3241 gggggagagg atcgcgcgcg tctcctggaa caggaacgac ttgttcgccg acccaccgcc
     3301 cttggcgaca aacatcaggt ggaactcgtc ggcgtggtgc tcgcccggcg cggcgaggat
     3361 gtcgaactgc accggcaggt tgttgccggt gttcacctcg tcgaacatgg tcagcggcgc
     3421 catctgcgag tcgcgcaggt tggtctcggt gtaggtgcgg tgcacacccc aggccagcgc
     3481 ctcctcctcg tcgccatcca cccagacgcg ctggcccttc ttgccgaaca cgatcgcggt
     3541 gccggtgtcc tggcacatcg gcagcacgcc gccggcggcg atgttggcgt tcttcagcag
     3601 gtccagcgcg acgaagcggt cgttcgcact cgcctccggg tcgtccagga tggcgcggag
     3661 ctgcgccagg tgagcgggcc gcagcaggtg cgacacgtca cggaaggcct ggaacgccag
     3721 ttcgctcagc gcgtccgggg cgatgcgcag cactgtctgg ccgtcgcagg tcgcggtgcg
     3781 cacgcccgcc accggcagcc gtttccaggc gatgcggtcc ggcccgagcg ggaacatcgg
     3841 cgcgtactgg aacccggacg ggcgcggcag cggagcgttc atggcgcatg atccttcatc
     3901 gagagccccg gccggcgacg cggctcggcc gcggcggaca tagcgaaccg gggctcagcc
     3961 gtccaacacg cccaggctgc gcagcagcgg cagccgctgg cgcaggaact gctgcaccag
     4021 gtcggccgcc aggcctccgg cttcggcggc atcggtcacc ggcttgccgt cgcgctgcaa
     4081 ggtgcgaccg gcgcgcgcca gcagggacag caggtccgcc accagcccct gcggttcggc
     4141 cggcttgccg gccagcaatt cgcccagcag cagcgtctcc acggcatcca ccgggatggc
     4201 ggatcccagc agcggcagtg ccacctgcgg cagctcgccg ccatcggcgt tcagcgccgc
     4261 gatggcctgg ttcagcgcgc gggtggactg ccgcaccgcc gcggagccgc cgtccggcag
     4321 cagcgggtgg gcatagcctc cggcgatcat caggctgatc gcctgcagca cttccaccgg
     4381 cgggcggccg gcgaaggcgt cgctggcctg caattcgccg acgctgaccg gcccttccgc
     4441 caggcggcgc tggatcggct gataggtctc cggacggccg ctgatcgtgc cgatcgcggt
     4501 attgaaggtg atcggctcgg gaaccgtctg cccggtggag gcgatggtga tggcgtccag
     4561 catcgcccga tgctcgccgg cggggatcgg ggtgatgccg cggcgataca agtcgcggcg
     4621 gaagccctgg caactggcga ggtcgcgcag tgtctcccgc agcaccgggt cgcggctttc
     4681 cgccagtatc tgcaccatct ccggcggcac cgaggcggcg tcgatgttct cggtcagcgt
     4741 tgcgctgccg atatagctgc atttgcactc cgccatctcc gacgcgatgt cggcgaagct
     4801 cagcgggtgc cagtcctggt tcaggaactc atgcgcgatg tagcgcgggt cctgccggcg
     4861 catgtcctgc agccgcggtt ccaggccggg gttgttggca aagaacaggg cgccggcggc
     4921 cttcaggcgg tcgatcatgt ccagcgcctc gaccgccgcc tggtcggcgc gggcggggct
     4981 ggcttcggcc agcatgcgca tcagcttgcg gagcggcacc atgctgctcc agccggcggt
     5041 gacgttgtag ctgagatagg ccagcccgcc gggccgcagc cgctgacgga tcaccgcctg
     5101 catgtgccgc cggtgctccg gggcgatcca gctcgccacg ccgtgcgaga ccatgaaatc
     5161 gaattccggc aggccgtccg gcggcagcgc ggcgaggtct gcgaaggcgg tctcgacgaa
     5221 ccgcacgttg cccagcccgg cgcgtgccgc caggtggcgt gccgtttcga tgtgcgccgg
     5281 gttgaagtcg aagccccaga cctcggcctg cgggctggtc gccgccacca ccagcgcggt
     5341 gaggccgttg ccgcaaccca gatcggcata gcggaacggc gcggccagcg aaggcgggcg
     5401 gtgacccagc agcagcgccg tggtggacag ccaggccggc gtggtttcgc gatggaaatt
     5461 gctggtatag accacgtccg taacgtagcc atcatcccat cgcgccatgc ctgaactccc
     5521 ccgagcggtg cgctgcacgc gcatcggcct gaggtatcag tccgtcggcg gattttccag
     5581 cgcggcggcg gcgcggcggg cttcgatctg cgcattgagt tcggcaccca gcagcaccgc
     5641 gtaggcggac acgtagaacc acagcatcac gccgatggcg gcgcccagcg gtccgtaggt
     5701 ggcgccgaat gtgccgagcc gcgagatgta gagcgacagc agggtggagg cgagcagcca
     5761 gagcgcggtg gcgacgatcg cccccggcgc gatcggctgg tgtggcggcg gcgtgcggga
     5821 cggaccgtag cggtacagca gggcgacggt gctgccgaac agccccacca gcatgatgac
     5881 gctggcggcc cggatcagcc cgctgttgta ggcggacagg ccgacgaagg cgatcaccac
     5941 cggcaggaac accagcaccg ccatggtcaa cccggccacc agcacggcgg ccagggtgat
     6001 gctcaacccg accgcctgga agcgtagcat ggggcgctgt tccgccacgt cataggcgac
     6061 attgatggcc gacagcatcg acttgctgcc ggtcgaggcg ctccagaagg tcagcagcag
     6121 tccaagcacc aggcccaggc tgacatggcc gtaatactgg ccgaccaact cgtgcacgcg
     6181 gccctcgatc agtgcgaagg ccggcggcgg cagcaactcg cccagcacct tgatctggcc
     6241 caggatatcg tcgcggttga acaccaggcc atagacggag atcaggatgc tgatcgccgg
     6301 gaacagcgcc agtgtcgcgt agaagccgca gccggcggcg gcgagcgaca tatggtcggt
     6361 gatgctctcg cggaacgcgg cccacaccat ccggcgccag gtcggccggg ggcggatcgc
     6421 ccggcggcgc gggcctcggg tccgctgtga tggaatgctg tccaagccgt tcctcaggtg
     6481 ttgcggggcg cagcgggaag cttgggaacg cgcggggcgc gcgccaaggg cggattttcc
     6541 ggccgagggg cattttccct attgcatggg gggtgggggg gtcctatgtg gcccccacgc
     6601 agcaacgcac gtgcctctgg ccctctgggg ttacgcaacg acgtcaagcg ttctggcaat
     6661 cgctctggtc gctggttcgt tcgaccgttt cgtcaactcc gcccgtcgcc tgattaggcg
     6721 ggcgctttgg taggggggcc cggtgcgatg acacccaaaa tcgcccgatt ccttgtgcag
     6781 cagcagccgg ccacgccgtg cctggtgctg gatctcgaac gcgtggaagc gaatttccgc
     6841 gcgctgcaac atgcgctgcc gctggcgcgc atctattacg ccgtgaaggc caatccagcg
     6901 ccgcaggtgc tggatcgcct ggtgcggctg agcagcagct tcgacgccgc cagcatcgag
     6961 gaagtgcgcg cctgcctgga cgccggtgct cggccggacg ccatcagctt cggcaacacg
     7021 atcaagaagg gctccgcgat ccgcgcggcg catgagcgcg gcgtgtcgct gttcgcc
//
LOCUS       DASSZH010000244         3438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_568058, whole genome shotgun sequence.
ACCESSION   DASSZH010000244 DASSZH010000000
VERSION     DASSZH010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568058"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="VFN42_05220"
     CDS             complement(<1..900)
                     /locus_tag="VFN42_05220"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HET8996049.1"
                     /translation="MLHVPSGPRVSLLRCRRSSVAIVVAMLAPVLAGAAGLGIEVSNW
                     SVEQMRMQRAADVAALAGAITYNNTVTNGGTTASAAQAAATAAANLAEINHVPGPTSP
                     SWDSTTQTLTDSNLTVKIVNGLRSASDTAIQVSVQESVPLMIARVVTANSSVTVSGTS
                     TAELLSNGNGSSTGPQPCVFAMSTASDAIGVTANGYISVTAPGCTLRSNSVINFSGGG
                     SFNTGGLYAAGDIDIPTWVSVTGGKYPDDGTVTDPYASNTSLQTVLTDASTVTGPSIA
                     CYNQWCKNGSTAMPTSSGSSFNGS"
     gene            complement(1059..2117)
                     /locus_tag="VFN42_05225"
     CDS             complement(1059..2117)
                     /locus_tag="VFN42_05225"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET8996050.1"
                     /translation="MMRHPEQWQAPIGAATVARTLRIIAILAAVVLVIWLLSDIVLLL
                     FLAVLIAVILRGVSDWGARHTGLGERAMLAVVTLAATALLAGFLYYIGPRLVSQSQDL
                     WGTMQDRVHHLQQAYGNTAWGHLVLNQLSSTTTQGKITTYASSLASFTVGGLATMFVL
                     IVTSLYFAISPMLYVDGVVRLFPLQYRPRARHVLLDIGSTLRWWSLGQLIDMIVVGVL
                     SGIGLALLGVPLALSLGVVAGLFTFVPYFGAIAAAVPAMLVALTISWQTSLWALVIFL
                     GCHGIEGYLISPLVQRRTVDLPPALTILSMTVLGTLFGPLGVILGTPVAAVLLVTVRE
                     AYVAEVLEDTEVSSLGQG"
     gene            complement(2185..>3438)
                     /gene="uvrC"
                     /locus_tag="VFN42_05230"
     CDS             complement(2185..>3438)
                     /gene="uvrC"
                     /locus_tag="VFN42_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET8996051.1"
                     /translation="SATVQQELAADMEQAAEHLEFERAAAVRDRIRALTFVQGSDVVN
                     PASLTDADVIAAYQTAGQTCVQVFFVRGGRNNGNRAFFPTHTKAEEAGEVLAAFVAQF
                     YDDKPPPPTLLLSHKLPEDALIAEALSLKAGRKVELLVPQRGEKHAVVKHAETNAREA
                     LERKLAESAGQAKLLDGVATLFGLPNPPQRIEVYDNSHIMGANPYGVMIVAGPEGFTK
                     AAYRKFGIRGPLTPGDDFAMMREVLQRRFSRALKEQAEGAPATDWPDIVLIDGGAGQL
                     SAARDVLDDLGVQDVKLIAIAKGPDRDAGREWFHTDGAEPFQLPPRDPVLYFLQRLRD
                     EAHRFAITTHRAGRSKAIRTSELDEIPGIGAARKRALLNHFGSARGVKMAGLTDLEAA
                     PGISRETARRVYAFFHPGVRLEERA"
BASE COUNT          549 a         1124 c         1218 g          547 t
ORIGIN      
        1 gctgccgttg aacgagctgc ccgagctggt gggcatcgcg gtgctgccgt tcttgcacca
       61 ctggttgtag caggcgatgc ttggcccggt gaccgtgctg gcgtcggtca gaacggtctg
      121 cagcgaggtg ttcgacgcgt agggatcggt caccgtcccg tcgtccggat acttgccgcc
      181 ggtaacgctg acccaggtgg gaatgtcgat atcacccgcg gcgtacaacc cgccggtgtt
      241 gaacgaaccg ccgcctgaga agttgatcac cgaattcgaa cgcagcgtgc agccgggggc
      301 ggtgacgctg atgtagccgt tcgcggtcac gccgatcgcg tcggacgccg tgctcatcgc
      361 gaacacgcaa ggctgcggcc cggtcgagga cccgttgcca ttgctgagca gttccgccgt
      421 ggacgttccg ctgaccgtca ccgagctgtt cgcggtgacg acgcgggcga tcatcaaagg
      481 cacgctctcc tgcaccgata cctggatcgc ggtatccgaa gcggatcgca ggccattgac
      541 gatcttcacc gtcaggttgc tgtcggtcag tgtctgcgtg gtggaatccc agctcggcga
      601 ggtggggccg ggaacgtggt tgatctccgc caggttggcg gcggccgtcg ccgcggcctg
      661 cgcggcggac gcggtggtcc cgccgttggt gacggtgttg ttgtaggtga tcgcgccggc
      721 cagcgccgcc acatcagcgg cgcgctgcat gcgcatctgc tcgaccgacc agttgctgac
      781 ctcgatcccc agccccgccg ccccggcaag caccggagcg agcatggcga ccacgatggc
      841 gaccgaggac cggcggcatc gcagcaggga gacgcgtggc ccggatggaa catggagcat
      901 gaaagtgctg cctggttgcc tgcgggagta atcaatcgca ggaactagtc cagacccggt
      961 aaccacccgg tttccatgat tggcgtcccg gccggccgga tcggctgttc agacggtatt
     1021 ccgggccgtt ccggcgtgat taacgagtta gtctgacgtc agccctgacc caggctgctg
     1081 acctcggtat cttccagtac ttccgccaca taggcctccc gcaccgtcac cagcagcacg
     1141 gcggccaccg gcgtgcccag gatgacgccc agggggccga acagggtgcc gagcaccgtc
     1201 atcgacagga tggtgagcgc gggaggcagg tccacggtgc gccgctgcac caggggggaa
     1261 atgaggtagc cctcgatgcc gtggcagccc aggaagatca ccagcgccca gagcgaggtc
     1321 tgccagctga tggtgagtgc caccagcatg gccgggaccg cggcggcgat tgcgccgaaa
     1381 tagggcacga aggtgaacag cccggccacc acgcccagcg acagggccag cggcaccccc
     1441 agcagcgcca ggccgatgcc gctcagcacg ccgaccacga tcatgtcgat gagctggccg
     1501 agcgaccacc agcgcagcgt actgccgatg tccagcagca catggcgggc gcgcgggcgg
     1561 tattgcagcg ggaacagccg caccacgcca tcgacgtaca gcatcggcga aatcgcgaaa
     1621 tacagcgagg tgacgatcag cacgaacatc gtcgccaggc cgcccacggt gaagctggcg
     1681 agcgagctgg cgtaggtcgt gatcttgccc tgcgtcgtcg tggacgacag ttggttcagc
     1741 accagatgcc cccaggcggt gttcccgtag gcctgctgca agtgatgcac ccggtcctgc
     1801 atggtacccc acagatcctg cgactgcgac accaggcggg ggccgatata atagaggaag
     1861 ccggccagca gcgcggtagc ggcgagggtc acaaccgcga gcatcgcgcg ttctcccagc
     1921 ccagtatgcc gcgcgcccca gtcggagacg ccgcgcagga tcacggcgat cagcacggcg
     1981 aggaagagca gcagcacgat gtccgacagc agccagatga ccagcacgac ggcggcgagg
     2041 atcgcgatga tccgcagtgt gcgggcaacc gtcgccgcgc cgatcggggc ttgccactgt
     2101 tccggatgcc gcatcatccc gccgtcctgt gttgcggggc aacaacgccg tggcggcggc
     2161 gcggttgggg gggcgctccc gcggttacgc ccgctcttcc agcctcacgc ccgggtgaaa
     2221 gaaggcgtaa acccggcgcg cggtctcccg gctgatgccg ggcgcggctt ccagatcggt
     2281 gaggccggcc attttcaccc cgcgggcaga gccgaaatgg ttgagcaggg cgcgcttgcg
     2341 ggcggcgccg atgccgggga tctcgtccag ctcgctggtg cggatcgcct tggagcggcc
     2401 ggcgcggtgg gtggtgatgg cgaagcggtg cgcctcgtcg cgcagccgct gcaggaaata
     2461 cagcaccggg tcgcgcggcg gcagctggaa cggttctgcc ccgtcggtgt ggaaccattc
     2521 gcggccggcg tcgcggtccg ggcccttggc gatggcgatc agcttcacgt cctgcacgcc
     2581 aaggtcgtcc agcacatccc gcgcggcgga gagctggccg gcgccgccgt cgatcagcac
     2641 gatgtccggc cagtcggtcg ccggcgcgcc ttcggcctgc tccttcagcg cgcgggaaaa
     2701 gcggcgctgc aatacctccc gcatcatggc gaagtcgtcg ccgggggtga gcgggccgcg
     2761 gatgccaaac tttctgtacg cggctttggt gaagccctcc ggcccggcga cgatcatcac
     2821 gccgtacggg ttggcgccca tgatgtggga gttgtcgtac acctcgatgc gctgcggcgg
     2881 gttgggcagg ccgaacaggg tggcgacgcc gtccagcagc ttcgcctggc cggcggactc
     2941 tgccaatttg cgctccagcg cctcgcgggc gttggtctcg gcgtgcttca ccacggcgtg
     3001 cttctcgccg cgttgcggca ccagcagctc caccttgcgg ccggctttca gtgacagcgc
     3061 ctcggcgatc agcgcgtctt ccggcagctt gtggctgagc agcagggtcg gcggcggcgg
     3121 cttgtcgtcg tagaattggg cgacgaaggc ggcgagcacc tcacccgctt cttccgcctt
     3181 ggtgtgggtg gggaagaagg cgcggttgcc gttgttgcgg ccgccgcgga cgaagaacac
     3241 ctgcacgcag gtctggccgg cggtctggta ggcggcgatc acgtcggcat cggtcaggct
     3301 ggccgggttc accacgtcgc tgccctgcac gaaggtcagc gcgcggatgc ggtcgcgcac
     3361 cgcggcggcg cgttcgaact ccaggtgttc cgcggcctgc tccatgtcgg cggcgagttc
     3421 ctgctgcacg gtggcgga
//
LOCUS       DASSZH010000245         7001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_278456, whole genome shotgun sequence.
ACCESSION   DASSZH010000245 DASSZH010000000
VERSION     DASSZH010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7001
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278456"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VFN42_05235"
     CDS             complement(<1..523)
                     /locus_tag="VFN42_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996052.1"
                     /translation="MPICEADPWRFQYFESVPCPEDVRVSTEDADSWLWYPQHRWIYD
                     KLAVALTQGLEAAPHGVMPAHFPVFSKPIMNLRGMGTGSQVIATADEYKAALTAGHFW
                     CTLLTGAHVSSDVALVDGEPRWWRHTTGEATPGGTFDHWTVHAGAMPEIEAWCGAWAR
                     QHLFGYTGMANFET"
     gene            complement(656..1435)
                     /locus_tag="VFN42_05240"
     CDS             complement(656..1435)
                     /locus_tag="VFN42_05240"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET8996053.1"
                     /translation="MAQTRPLDPATIRVHRNGSGPVLVMLHCLGMTHHLWDCLGGLSD
                     RFTLLSYDLPGHGETPVPDAPYGVEELSAQLAGVLRREGVSRAHVMGISLGGLVTQCF
                     AATEPDMVDRIVLCDTTPRYTDEARGMWAVRAAGARKDGPVSLLPNIEKIWFTEPFLK
                     QSPPAVQLVRDTFRACSGEGYALACEALAAAELRPLAPRIKAPALVVCGAEEGAAFLD
                     AAHWLGGNIAGARLEFVPNAAHASVLEQPERIEHLVREFLA"
     gene            complement(1457..3070)
                     /locus_tag="VFN42_05245"
     CDS             complement(1457..3070)
                     /locus_tag="VFN42_05245"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF002550.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HET8996054.1"
                     /translation="MADYDYIIIGAGSAGCVLANRLTENASTRVLLLEAGPKDDSMWI
                     NIPAGFTKLLNSPVYNWNFATEPEDNVKGRSIPIPRGRTLGGSSAINGMLYVRGNHLD
                     YDTWAQFGNRGWSYESVLPYFRRSENFEGDGDAARGRGGPLNVAHMREHAELTDAFID
                     AAVAEGYPRNPDYNNGNQEGFGYFQVTQKDGRRWSTARAYLDPARNRPNLHVETDAHA
                     TRILLEGKRAVGVAYLQGGQQREARAGAEVIVAAGAVKSPHLLELSGLGQPELLQSLG
                     IAVQHELRGVGENYRDHYAPRMNWRVNQPITLNEQTRGTAFLKEIARYYLQHRGVLTF
                     TAGIAYGFVKTRPELEVPDVQFHFAHASYGSAQTRVLDSEPGMTLTTYQCRPESKGSI
                     HAKSPDAMAAPAIRPNFLAEKLDQDTVVAGLKIGRSIIGNRAIGRYVAYEMNPGPQYQ
                     TDAELLEFARINGQTTYHVIGTCKMGGDPMAVVDDQLRVHGIAGLRVVDASIMPTMVS
                     GNTNAAVIMIAEKGADMIKAAARDLRAAA"
     gene            complement(3130..4677)
                     /locus_tag="VFN42_05250"
     CDS             complement(3130..4677)
                     /locus_tag="VFN42_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007398750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HET8996055.1"
                     /translation="MTRGELDPITVEVIGSALSSIVEEMGEALIRASCSTNIKERRDC
                     STALFDLHGRTLCQAEHIPMHLGSFLDLIPHIMRRHELAEMQPGDVFVGNDAYEGGGT
                     HLPDIVLAEPVFVDGTIIAWAVNLAHHADFADRGHAHIYQEGLRIPPIRLYRAGELQT
                     DVQELILLNCQVPRERLSDFRAQMAANRLGVQRLQALCRKYGRDTTLAAGAALQDYAE
                     RKMRAGIAQMQDGTYRFSDRYDCPEIDGELDLSCAITVAGEEMHLHFEAPPQQRAGLN
                     MTYTALLSTVYYAVKTLVDPSILPNAGLARPLHVTAPAGSVVNCLHPAAVNGRLSLCQ
                     RVVDLIFGALAPVLPDRVIAASNGACFSITFAGHQPHDNALWVYLETIGGGAGARHAK
                     DGLDGVHVHMTNTSNLPVEALEGEYPLTLLRYELVEGSGGAGRHRGGMGLRRVYRAEH
                     ECQVRVDGSRLLSPPWGLFGGLPGGKADIVAGGGARPFDHGRGTLRAGETIAVITAGA
                     GGYGPPD"
     gene            complement(4674..6725)
                     /locus_tag="VFN42_05255"
     CDS             complement(4674..6725)
                     /locus_tag="VFN42_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HET8996056.1"
                     /translation="MAWRVGVDSGGTFTDVCLFDDATGQLAVWKLASTPDDPSRGIAE
                     GVAQALSRQDAPPANVGYFGHGTTVATNALIQHRGVRTGLITSDGFRDLLEIGRQKRP
                     DLYDLQADKPPTLVPRHLRLEVPERVRYDLSVEIPLDEMAFRAAVRVLREAGVAAVAV
                     CFLYAFAATGHEAAARRILAEEFPQAFACVSHDVAAEFREFERLSTTVVNAYLGPVMH
                     GYIQRLGERLEALGVPAQPHLTQSNGGVIGFAEAARLPVRTVLSGPATGVIGAQAVGR
                     TAGFTNLITFDMGGTSSDVALLQDGVCRLTSEAMVHGYPIKAPMLDIHTVGAGGGSIA
                     FVDSGGLLKVGPGSAGADPGPVCYGRGGTQPTVTDANVLLGTLNPAYLLGGRMPVRRD
                     LAETAIAGLAATLGLAPMATAQGIVSVVTASMARAIRVVSVQRGHDPRDYTLVAFGGA
                     GPLHAARLARELDLGRVLVPRNPGILCAMGLLLTDLRADFSATRLCSLRAEALDDVAA
                     VFAGLAEQAQAWFAAESIAADARHMQRSVDMRYAGQNYELSVPLPDDAIGPATLDALA
                     AGFAAAHQRMYGFVADEEPVQLVTFRLEASGLVPKAQFTAQPDTGPDASVAIITDRDV
                     WLPEAGGFVRCRVFDRDRLHAGNRIAGPAVVEQMDATTLVLPGMVASVDPYLNLILEA
                     A"
BASE COUNT         1110 a         2402 c         2345 g         1144 t
ORIGIN      
        1 tcgtctcgaa attcgccatg ccggtgtagc cgaacaggtg ctggcgcgcc caggcgccgc
       61 accacgcctc gatctccggc atggccccgg cgtgcacggt ccagtggtcg aaggtgccac
      121 cgggcgtggc ctccccggtg gtgtgccgcc accagcgcgg ctcgccatcc accagcgcga
      181 catcgctgct gacatgggcg ccggtcagca gcgtacacca gaaatgcccg gccgtcagtg
      241 cggccttgta ttcgtcggcg gtggcgatca cctggctgcc ggtgcccatt ccgcgcagat
      301 tcatgatcgg cttggagaac accggaaagt gggccggcat gacgccatgc ggcgccgcct
      361 ccaggccttg cgtcagcgcc actgcgagct tgtcgtagat ccagcggtgc tgcgggtacc
      421 acagccagga atccgcgtct tcagtggaga cgcgcacgtc ctccgggcag gggacgctct
      481 cgaaatactg gaaccgccat ggatcggctt cgcagatcgg catgcgtctc tccccggaca
      541 tccgagtcgg cgatcgtaat ggcagaagcg aggcggcgcc gccaagttgc cgtgagcagg
      601 cgatccggaa acgccgcccg ccggccagcc ggcgggcagc cgcgttcggc cgcgcttagg
      661 cgaggaattc ccgcaccaga tgttcgatcc gttccggctg ctccagcacg gaggcgtgcg
      721 ccgcattcgg gacgaattcc agcctggcgc cggcgatgtt gccgcccagc cagtgggccg
      781 catccaggaa ggcggctcct tcttccgcgc cacagaccac cagcgccggt gccttgatgc
      841 ggggcgcgag cgggcgcagt tcggcggcgg ccagggcctc gcaggccagc gcatagccct
      901 cgccgctgca ggcgcggaac gtgtcccgca ccaactgcac cgcgggcggc gactgcttca
      961 ggaacggctc ggtgaaccag atcttctcga tgttgggcag cagcgagacc ggcccatcct
     1021 tgcgcgcgcc cgcggcgcgc actgcccaca tgccgcgcgc ctcatcggtg tagcgcgggg
     1081 tggtgtcgca cagcacgatg cggtcgacca tgtccggttc cgtcgcggcg aagcactgcg
     1141 tcaccagccc gccgagcgaa atgcccatga cgtgtgcacg ggagacgccc tcccggcgga
     1201 ggacgccggc cagttgggcg gacagttcct cgacgccgta cggcgcgtcg ggcaccggcg
     1261 tttcgccgtg gccgggcaga tcgtaggaca gcagcgtgaa gcggtcgctc aggccgccca
     1321 ggcagtccca cagatgatgc gtcatgccaa ggcagtgcag catcaccaga accggcccgc
     1381 tgccattgcg gtgcacccgg atcgtcgcgg gatccagcgg tcgtgtctga gccattgtcg
     1441 tttcctcccc tgctttctat gccgcggcgc gcaaatcccg cgctgcagcc ttgatcatgt
     1501 cggcaccttt ctccgcgatc atgatcaccg cggcattggt attgcccgat accatggttg
     1561 gcatgatcga cgcgtcaacc acgcgcagcc cggcgatgcc gtgcacccgc aattggtcgt
     1621 ccaccaccgc catcgggtcg ccgcccatct tgcaggtgcc aatcacgtgg taggtggtct
     1681 ggccgttgat gcgggcaaac tcgagcagct ccgcatcggt ctggtattgc gggccggggt
     1741 tcatctcata ggcgacatag cggccgatcg cgcgattgcc gatgatggag cggccgatct
     1801 tcaggccggc aaccacggtg tcctggtcca gcttttccgc caggaagttc gggcggatcg
     1861 ccggggcggc catcgcgtcc ggcgatttgg cgtggatcga acctttcgat tccggccggc
     1921 actgataggt ggtcagcgtc atgcccggct cgctgtccag cacgcgtgtt tgtgccgaac
     1981 cgtagctggc atgcgcgaag tggaattgca cgtcagggac ttccagttcc ggacgcgtct
     2041 tgacgaagcc gtacgcgatg ccggcggtga aggtcagcac accgcggtgc tgcaggtagt
     2101 accgggcgat ctccttcagg aaagcggtac cccgcgtctg ctcgttcagc gtgatcggct
     2161 ggttcacccg ccagttcatg cgcggcgcat agtggtcgcg atagttctcg ccgacaccgc
     2221 gcagttcgtg ctgcacggcg atccccaggg actgcagcag ttccggctgg ccgaggccgg
     2281 acagctccag caagtgtggc gacttcaccg cgccggcggc gacgatcacc tcggcgccgg
     2341 cccgcgcctc ccgttgctgg ccgccctgga gataggcgac gccgaccgcg cgcttgccct
     2401 ccagcaggat gcgcgtggca tgcgcgtcgg tctccacgtg caggttcggc cggttgcgcg
     2461 ccgggtcgag ataggcgcgc gccgtggacc agcggcggcc atccttctgc gtcacctgga
     2521 agtagccgaa accttcctgg ttgccgttgt tgtagtcggg attacgcgga tagccctcgg
     2581 ccaccgcggc gtcgatgaag gcatcggtga gttccgcgtg ttcacgcata tgcgcgacgt
     2641 tcagcggtcc gccgcggccg cgcgcggcat ccccgtcgcc ctcgaagttt tccgatctgc
     2701 ggaaataggg caacaccgat tcgtacgacc agccgcggtt gccgaactgc gcccacgtat
     2761 cgtagtcgag atgattgccc cggacataca gcatgccgtt gatcgcgctg gagccgccca
     2821 gcgtgcggcc gcgcgggatc gggatcgaac ggcccttcac gttgtcttcc ggctcggtgg
     2881 cgaagttcca gttgtaaacg gggctgttca gcagcttggt gaagccggcc gggatgttga
     2941 tccacatgga atcatccttc ggcccggcct ccagcagcag cacccgggtg ctggcatttt
     3001 ccgtcagccg gttggccagc acgcacccgg cagagccggc gccgatgatg atgtagtcgt
     3061 agtccgccat gcccgtttcc ccttcaccca cgcggtcatc cgtcttcgtg tgacggcggc
     3121 gggagcgagt caatcgggcg gcccgtagcc gccggccccg gcggtgatca ccgcgatggt
     3181 ttcacccgcc cgcagcgttc cgcgcccgtg atcaaacggc ctggcaccgc cgccggcaac
     3241 gatgtccgcc ttgccgcccg gcaggccgcc gaacaatccc cagggcggcg acagcaggcg
     3301 ggagccatcc acccgcacct ggcattcgtg ttccgcgcgg tagacccggc gcaatcccat
     3361 cccgccgcga tgccgcccgg caccgcccga accttccacc aattcgtagc gcagcagggt
     3421 cagcggatac tcgccttcca gcgcctccac cggcaggttg gaggtattgg tcatgtgcac
     3481 gtgcacgcca tccagcccat cctttgcatg gcgcgcgccg gcgccaccgc cgatggtttc
     3541 cagatagacc cacaacgcat tgtcgtgcgg ctgatgtccg gcgaaggtga tggaaaagca
     3601 ggcgccgttg ctggccgcga tcacccggtc cggcagcacc ggtgccaatg caccgaagat
     3661 caggtccacc acgcgctgac acaggctcag cctgccattc actgccgcgg gatgcaggca
     3721 gttgaccacg ctgccggccg gcgccgtgac gtgcagcgga cgcgccaggc cggcattcgg
     3781 cagaatgctc ggatccacca gcgtcttcac cgcgtagtac acggtggaca gcagcgcggt
     3841 gtaggtcatg ttcagcccgg cgcgctgttg cggcggtgct tcgaagtgca ggtgcatttc
     3901 ctcgccggcc accgtgatgg cgcaggacag gtcgagttcg ccatcgatct ccgggcagtc
     3961 gtagcggtcg ctgaaccggt aggtgccgtc ctgcatctgc gcgatgccgg cgcgcatctt
     4021 gcgctccgcg tagtcctgca gcgcggcgcc ggcggccagc gtcgtgtcgc ggccgtattt
     4081 cctgcacaac gcctgcaggc gctgcacgcc gagccggttg gccgccatct gggcgcggaa
     4141 gtcggacagg cgctcgcgcg gcacctggca gttgagcagg atcagctcct gcacgtcggt
     4201 ctgcaattcg ccggcgcggt acagccggat cggcgggatg cgcaggcctt cctggtagat
     4261 gtgcgcgtgg ccccgatcgg cgaaatccgc atgatgcgcc aggttcaccg cccaggcgat
     4321 gatcgtgcca tcaacgaaga cgggttcggc cagcacgata tccggcagat gcgtgccgcc
     4381 gccttcatag gcgtcgttgc caacgaacac gtcgcccggc tgcatctccg ccagttcatg
     4441 ccggcgcatg atgtgcggga ttaggtcgag aaagctgccc agatgcatcg ggatgtgctc
     4501 ggcctggcac agcgtcctgc cgtgcagatc gaacagcgcg gtggagcaat cgcggcgttc
     4561 cttgatgttg gtggagcagg aggcgcggat cagcgcctcc cccatttcct cgacgatgga
     4621 ggacagcgcg ctgccaatca cctcgacggt gatcgggtcc agctcccccc gtgtcatgcg
     4681 gcctccagga tcaggttcag gtagggatct accgacgcca ccatgccagg cagcaccagc
     4741 gtggtcgcat ccatctgctc gacgacagcc ggacctgcga tgcggttgcc ggcatgcagc
     4801 ctgtcgcgat cgaacacgcg gcagcgcacg aatccgccgg cttccggcag ccacacatcg
     4861 cgatccgtga tgatggcgac ggacgcatcc ggtccggtgt ccggctgggc agtgaactgc
     4921 gctttgggca ccaggccgga ggcctccagg cggaaggtga cgagctgcac cggttcctca
     4981 tcggccacga agccgtacat ccgctggtgc gcggccgcga agccggcggc caatgcgtcc
     5041 agggtggccg gaccgatcgc gtcatccggc aacggcaccg acagttcgta gttctgtccg
     5101 gcatagcgca tgtctacgct gcgctgcata tggcgcgcat ccgccgcgat gctttcggcg
     5161 gcgaaccacg cctgcgcctg ttcggccagt ccggcaaaga ccgccgcaac atcgtccagc
     5221 gcctccgctc gcaggctgca caaccgggtg gcggagaagt cggcccgcag atcggtcagc
     5281 agcaagccca tcgcgcagag gattccggga ttgcgtggca ccaggacgcg gccgaggtcg
     5341 agttcgcggg ccagccgggc ggcatgcaac gggccggcgc cgccgaacgc caccagcgtg
     5401 tagtcgcgcg gatcgtgtcc ccgctgcacg ctgaccaccc ggatggcgcg cgccatgctc
     5461 gcggttacga cggacacgat cccttgcgcc gttgccatcg gcgccaggcc aagcgtggcg
     5521 gcaaggccgg caattgccgt ctccgccaaa tcgcgccgca ccggcatgcg gccacccagc
     5581 agataggcag ggttgagggt gccgagcagc acgttggcgt cggtgacggt cggctgcgta
     5641 ccgccgcgtc cgtagcagac cgggccggga tcggcgccgg cgctgcccgg gccgaccttc
     5701 agcagcccgc cgctgtcgac aaaggcgatc gagccgcctc ccgcgcccac ggtatggatg
     5761 tcgagcatcg gcgccttgat cggatagccg tgcaccattg cctcactggt cagccggcag
     5821 acgccgtcct gcagcagagc gacatcgctg gaggtgccgc ccatgtcgaa cgtgatcaga
     5881 ttggtgaagc cggcggtccg gccgaccgcc tgggcgccga tcacgccggt tgcaggtccc
     5941 gacagcacgg tgcgcaccgg cagacgggcc gcctcggcga agccgatcac gccgccgttc
     6001 gactgtgtca gatgcggctg cgccggcacg cccagcgcct ccaggcgctc gcccagccgc
     6061 tggatatagc cgtgcatcac cgggccaaga taagcgttca ccacggtggt ggagaggcgc
     6121 tcgaattcgc gaaactccgc ggcgacgtcg tgcgacacgc aggcgaaggc ttgcgggaat
     6181 tcttccgcca ggatccggcg cgcggcggct tcgtggccgg tggcggcgaa cgcatacagg
     6241 aagcacaccg ccactgcggc gacgccggcc tcgcgcaaga cgcgtacggc ggcacgaaac
     6301 gccatttcgt ccagcgggat ctccaccgag aggtcgtaac ggacgcgctc gggcacttcc
     6361 aggcgcaggt ggcgaggcac gagggtcggc ggcttgtcgg cctgcaggtc gtacagatcg
     6421 gggcgcttct ggcggccgat ctccagcagg tcgcgaaatc cgtcgctggt gatcagcccg
     6481 gtcctcacgc cccggtgttg gatcagcgcg ttggtcgcca cagtggtgcc gtggccgaaa
     6541 tatcccacat tggccggcgg cgcgtcctgg cgggacagcg cctgcgccac gccctcggcg
     6601 atgccgcgcg atgggtcgtc gggtgtggag gcgagcttcc acaccgcaag ctgacccgtc
     6661 gcatcatcga acaggcagac gtccgtgaag gtgccgccgg aatcgacgcc aacccgccag
     6721 gccatgatgc gcaatgctcc agtgtggaag cctcaatgct cgcccacgca ggggtatcct
     6781 atggtcagca cgcacaactg gccaattccg aggatgttcc cgattcaacc ggtcactgtc
     6841 atcccatcgc gcagccctgg aggggcggcc tgatgtcgcc gccggtccag ccggtcgcgt
     6901 ccgacgccgc cctgccaccc cgcaccgggg tggtgattgt cggtggcggc atcatcggtg
     6961 tgtccaccgc gctgttcctg gcggagaagg gccacgcggt g
//
LOCUS       DASSZH010000246         3022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1250108, whole genome shotgun sequence.
ACCESSION   DASSZH010000246 DASSZH010000000
VERSION     DASSZH010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250108"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="VFN42_05260"
     CDS             <1..746
                     /locus_tag="VFN42_05260"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HET8996057.1"
                     /translation="MWRDGQHATLGWLSVSHGFTAEVMARQGFDALCVDLQHGTSEMK
                     DVGPMLQAISQTDTVPVVRVPWNEPAAIMKALDLGAYAIIVPLVSTAEEAAKAVAACR
                     YPPVGMRSSGPVRAIHYGGADYQAKANDEIVVMAMIETKEGIENLDAICATPGLDAVY
                     IGPADLSFALGLPPRGDNPDPTHLATCDKILASAHKHGIKCVMHCASAAFAAGAVKRG
                     FDMVMLTSDLSCMIAGARMQLDELKAKTA"
     gene            complement(992..1207)
                     /locus_tag="VFN42_05265"
     CDS             complement(992..1207)
                     /locus_tag="VFN42_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HET8996058.1"
                     /translation="MRIIIGIPDGQAQDLTDLAARLKQARAALVRQAITEYLARHRRN
                     SRVDAFGLWGADAPDGVTYQHRIRGAW"
     gene            1358..2533
                     /locus_tag="VFN42_05270"
     CDS             1358..2533
                     /locus_tag="VFN42_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8996059.1"
                     /translation="MLSDQIDPPLPPLDPALLPPGVRPRFIDGINGLRVHLLEAGFEP
                     PTRPMVVLLHGFPELAFSWRKVMPLLAEAGFHVIAPDQRGYGRTTGWSPEYDTDLSPF
                     RLLNLARDVLELVFRFGRRSVAAVVGHDFGSPVAAWCALTRPDVFRRVALLSAPFGGP
                     PALRFGPDAPGQAPADIHAALARLDPPRQHYQWYYSTRPANADMHHPPQGLHDFLRAY
                     FHHKSGDWPGNRPFQLAGWIAPELAKLPTYYVMQLHRTMPETVAEAMPSPEQIAACAW
                     LPERDLAVYAAEYGRTGFQGGLNWYRCRTSGRDEAELGLFSGRTIDVPAMFIAGARDW
                     GIHQVPGALERMQHQACTRMTACRLVPGAGHWVQQERASEVAAHLLDLLGEAEPASP"
     gene            complement(2543..>3022)
                     /locus_tag="VFN42_05275"
     CDS             complement(2543..>3022)
                     /locus_tag="VFN42_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HET8996060.1"
                     /translation="LKALTGLFLEVGRDVAQEYEGQVEMNERIVDACAMQLVLNPWQF
                     DVLVTTNLFGDILSDLMAGLVGGLGMAPGANIGRDAAIFEAVHGSAPDIAGQGIANPL
                     ALLLAAGLMLEHIDCAPLARRLRSAIDVTVNQDQVRTRDLGGQASTSDFAQAVARRV"
BASE COUNT          476 a         1044 c         1018 g          484 t
ORIGIN      
        1 agatgtggcg cgatggccaa cacgcgacgc tgggctggct ctccgtctcc cacggcttca
       61 ccgccgaggt catggcccgc cagggcttcg acgccctgtg cgtcgatctg cagcacggca
      121 cgtcggagat gaaggacgtc ggccccatgc tgcaggcgat ttcgcagacc gacacggtgc
      181 cggtggtgcg ggtgccgtgg aacgaaccgg cggccatcat gaaggcgctg gacctgggcg
      241 cctacgccat catcgtgccg ctggtcagca cggcggagga agcggcgaaa gcggtggccg
      301 cctgccgcta tccgccggtg ggcatgcgct cgtcgggacc ggtccgcgcc atccactacg
      361 gcggcgcgga ctatcaggcc aaggcgaatg acgagatcgt cgtcatggcg atgatcgaga
      421 ccaaggaggg catcgaaaac ctcgatgcga tctgcgccac gcccggcctc gacgcggtgt
      481 atatcgggcc ggccgacctg tccttcgcgc tgggcctgcc gccgcgcggc gataacccgg
      541 acccgacgca cctggccacc tgcgacaaga tcctggcgag cgcgcacaag cacggcatca
      601 aatgcgtcat gcactgcgcc agcgcggcgt tcgcggcggg ggcggtgaag cgcggcttcg
      661 acatggtgat gctgacgtcc gatttgtcgt gcatgatcgc cggcgcgcgg atgcagttgg
      721 acgagctgaa ggcgaagacc gcctgatacc atctcgcctt ggtggtgaca caccggccgc
      781 ggtaccccgt cgtggcggcc cttcggggct gtaatccaat tgcgcggaca cgccgggacg
      841 cagcgtacaa cgtcgtggcg ggcgaaggcc cgccacccac gactttcctg ggtacaactg
      901 ggaaaagtcg tggatgccgg ccttcgccgg catgacgagg gtcggcatgc cggcgcgcac
      961 gcactcagca cgttggtgtt gagtcggggc ttcaccatgc gccgcggatg cggtgctgat
     1021 aggtgacccc gtccggcgcg tcagcacccc acaggccgaa ggcgtcaacc cgcgagtttc
     1081 gccggtggcg ggcgaggtat tcggtgatcg cctgccgcac caatgcagcg cgcgcttgct
     1141 tcaaccgcgc ggcgaggtcc gtgaggtcct gcgcctggcc gtccgggatg ccaatgatta
     1201 tgcgcatagc tctcgctatc agatggcgga ataagtcatc aagccaggtg cgcgtaatcc
     1261 agttagctgc cggagcgtgc tactgtcgcg agcggggata acctctatct tatgccccga
     1321 cgtacggccc aaaaggcata aaggctgaaa gctctcaatg ctctctgatc agatcgaccc
     1381 cccgctgccc ccgctcgacc cggcgctgct gccgcccggt gtgcggccgc gcttcatcga
     1441 cggcatcaac ggtctgcgcg tacatctgct ggaagctgga ttcgaaccgc ccacacggcc
     1501 aatggtggtg ctgctgcacg gctttcccga actcgccttc tcctggcgca aggtgatgcc
     1561 gctgctggcc gaggccgggt tccatgtcat cgcgccggac cagcgcggct atggccgcac
     1621 caccggctgg agcccggaat acgataccga cctgtcgccc ttccgcctgc tcaacctggc
     1681 gcgcgacgtg ctggagctgg tgttccggtt cggccggcgc agcgtcgccg ccgtggtcgg
     1741 ccacgatttc ggctcccccg tcgcggcctg gtgcgcgctg acccggccgg acgtgtttcg
     1801 ccgcgtcgcg ctgctgagcg cgccgttcgg cggcccgccc gcgctgcgct tcggtccgga
     1861 tgcgccgggg caggcgccgg ctgatatcca cgcggcgctc gcgcggctcg atccgccgcg
     1921 ccagcattac cagtggtact attcaacccg tccggcgaac gccgacatgc atcatccgcc
     1981 gcagggattg catgatttcc tgcgcgccta tttccaccac aaaagcggcg actggccggg
     2041 caaccggccg ttccagctgg ccggctggat cgcgccggaa cttgccaaac tgccgaccta
     2101 ttacgtcatg caactgcatc gcacgatgcc ggagacggtc gcggaggcga tgccctcacc
     2161 cgagcagatc gccgcctgtg cctggctgcc cgagcgggac ctggcggtct atgccgccga
     2221 gtatggccgt accgggttcc agggcgggct gaactggtac cgctgccgca cctccgggcg
     2281 ggacgaggcg gaactgggcc tgttcagcgg acgcaccatc gacgtgcccg cgatgttcat
     2341 cgccggtgcg cgcgactggg gcattcacca ggtgccgggc gcgctggagc gcatgcagca
     2401 tcaggcctgc acccgcatga ccgcatgccg gctggtgccc ggagccgggc attgggtgca
     2461 acaggagcgt gcgtccgagg tcgccgccca cctgctggac ctgctcgggg aggcggaacc
     2521 cgccagcccc tgaccagccc cgctacaccc ggcgggcgac agcctgcgcg aagtcgcttg
     2581 tggaggcctg gccgccgagg tcgcgagtgc gcacctggtc ctggttcacc gtgacgtcga
     2641 tcgccgagcg cagccgccgc gccagcgggg cgcagtcgat atgctccagc atcagccctg
     2701 ccgccagcag cagtgccagc ggattggcga tgccctggcc ggcaatgtcg ggggcagagc
     2761 cgtgcaccgc ctcgaagatc gcggcatcgc ggccgatatt ggcgcccggc gccatgccca
     2821 gcccgccgac cagcccagcc atcaggtcgg acaggatgtc gccgaacagg ttcgtggtga
     2881 ccagcacgtc gaactgccag gggttcagca ccagctgcat ggcacaggca tcgacgatgc
     2941 gctcgttcat ctcgacctgg ccctcgtatt cctgggcgac gtcgcggccg acttccagga
     3001 acaacccggt cagcgccttc ag
//
LOCUS       DASSZH010000247         2630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1233240, whole genome shotgun sequence.
ACCESSION   DASSZH010000247 DASSZH010000000
VERSION     DASSZH010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233240"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2251
                     /locus_tag="VFN42_05280"
     CDS             <1..2251
                     /locus_tag="VFN42_05280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8996061.1"
                     /translation="EAHVSYAQRLSGVAPLPGSSRPTAWPPFFGDLLLRQGLRWARAV
                     QCRWPRCGTLLRRLVLLVWWSCTLQLGLHLRSWLRARQLRRAAPPPLALPLIDAVQPA
                     ALVVPAQEHPLVSVIVPTYGKVEYTLRCLASIAAHAPAAAIEVIVVDDAYPGADIACL
                     QQVRGIRLLRSPVNRGFLHSCNAAAAQARGRWLLFLNNDTQVLPGWLDSMLALLERRA
                     DVGAVGSKLLYPDGRLQEAGGIIWDDGSGWNYGRGDNPALPQYNYVREVDYCSGASLM
                     VRRDVFLRLDGFDACYAPAYFEDTDLCFRLRAMGLQTLYQPRSQVVHFEGVSHGRDIA
                     AGVKAFQAVNCRMFLRQWGPSLGRCHYPNGTHVLRARERGRGRGVALVVDHKVPEPDR
                     DAGSQAILGVLRTLLAEGVLVKFWPANLAYVAGYTEALQQMGVEVLHGPHQAPMASWL
                     HEHGAELDLVVLSRPDVAEPWLDAVRTYSRARVFYYGHDLHCSRMRMQAAVTRDARLL
                     AEAACMEERERAIWRQVDASLYLSQAEADAVRRLEPQAVVHAVVPYSFDSFAEPRQAP
                     GGRDILFVAGFAHPPNEDAARWFVTEVLPRVHAGVAGVRLLIAGSNPGPGVLALAREA
                     VQVLPNLDHAALRDCYRHARVAVVPLRCGAGVKLKVVEALREGVPLVTTTVGAQGLPG
                     LDRVAFIRDDPAGFADAICALLLGDALWRSRCAAQLSYAGARFSEPALRDSLRSVLGL
                     AEAAWARAA"
BASE COUNT          357 a          814 c          967 g          492 t
ORIGIN      
        1 ggaggcccat gtgagttacg cccagcgcct gtccggtgtc gcgccgctgc caggcagttc
       61 gcggccgaca gcatggccgc cattcttcgg cgacctgctg ttgcgccagg gcctgcgctg
      121 ggcgcgggcg gtgcaatgcc gctggccgcg gtgcggcacc ttgctgcgcc ggctggtgct
      181 gctggtctgg tggagctgca cactgcagct cggtctccac ctgcgcagct ggttgcgggc
      241 acggcagctg cgccgagcgg caccaccccc gctggccttg ccgctgatcg atgcggtgca
      301 gccggcggcc ttggtcgtgc cggcgcagga gcacccgctg gtgtcggtga tcgtgccgac
      361 ttatggcaaa gtggaataca cgctgcgctg ccttgcctcg atcgcggccc acgcaccggc
      421 ggcggcgatc gaagtcattg tggtggacga tgcgtatccc ggcgcggaca tcgcctgtct
      481 gcagcaggtc cgcggcattc gtctgctgcg cagtccggtt aatcgcggct ttctgcacag
      541 ttgcaatgct gcggcagcgc aggcgcgcgg gcgctggctg ctgttcctga acaacgacac
      601 gcaggtgctg ccgggttggc tcgacagcat gctggcgctg ttagaacgtc gcgcggatgt
      661 tggcgcggtg ggttccaagc tgctgtatcc ggacggacgg ctgcaggaag ccggaggcat
      721 catctgggac gatggttccg gttggaacta cggccgcggc gacaaccccg ctctgcccca
      781 gtacaactac gtgcgcgaag tggattactg ctccggcgca tcgctgatgg tgcggcggga
      841 cgtgttcctg cggctggacg gattcgatgc gtgctatgcg ccggcctatt tcgaggatac
      901 cgacctgtgc ttccgtctgc gggcaatggg attgcagacg ctgtaccagc cgcgatcgca
      961 ggtggtgcat ttcgagggcg tgtcgcatgg ccgcgacatc gccgccggtg tgaaggcgtt
     1021 ccaggcggtg aactgccgca tgttcctgcg gcagtgggga ccgagcctgg gccgctgtca
     1081 ttaccccaac ggcacgcatg tgctgcgcgc gcgggagcgg ggcagggggc gcggcgtcgc
     1141 cctggtggtg gaccacaagg tgccggagcc cgatcgtgat gccggctccc aggcgatcct
     1201 tggcgtgctg cgcacgctgc tggcggaggg tgtgctggtg aagttctggc cggccaacct
     1261 cgcctatgtt gccggctata ccgaagcgct gcagcagatg ggggttgagg tgctgcatgg
     1321 tccgcaccag gcgccgatgg cctcctggtt gcacgagcat ggggcggaac tggatctggt
     1381 ggtgctcagc cggccggacg ttgcagaacc ctggctcgac gcggtgcgga cgtacagcag
     1441 agcccgggtg ttctattacg gacatgatct gcattgcagc cggatgcgca tgcaggccgc
     1501 ggtgacgcgg gatgcccggc tgctggccga ggcggcgtgc atggaggagc gcgaacgcgc
     1561 catctggcgg caggtggatg cgtcgctgta cctgtcgcag gctgaggctg atgccgtgcg
     1621 ccgcctggaa ccgcaggccg tggtacacgc cgtggtgccg tacagtttcg acagtttcgc
     1681 ggagccgcgg caggcgcccg gtggccgcga catcctgttc gtcgccggct tcgcgcatcc
     1741 gccgaacgag gatgcggcgc gctggttcgt gaccgaggtg ttgccgcgtg tgcatgccgg
     1801 cgtcgccggc gttcggctgc tgatcgccgg ttccaacccc ggccccggcg tactggcgct
     1861 ggcgcgggag gcagtgcagg tgctgccgaa cctggatcac gccgcgctgc gcgattgcta
     1921 tcgccatgcg cgcgtcgcgg tggtgccgct gcgctgcggt gccggcgtga agttgaaggt
     1981 ggtggaggcg ctgcgggagg gcgtgccgct ggtcaccacg acggtgggcg cgcaggggct
     2041 gccgggactg gaccgggtcg ccttcatccg cgacgacccg gccgggttcg ccgatgccat
     2101 ctgtgcgctg ctgctcggcg acgcgctgtg gcggtcgcga tgtgccgcgc agctaagcta
     2161 tgctggcgcg cggttcagcg agcctgcgtt gcgggactcg ctgcgttcgg tcctgggatt
     2221 ggcggaggcg gcgtgggcgc gcgcggcata gcggctgccg tgtcctgcgc ccggcctcag
     2281 ccgaaccgcg aatgacctga tgaactcgcc cgcatgacga agccgcaggc gagagttttg
     2341 ccaactatca tctctactct accggctccc gaccttctgt atatcgcaac cgtgtcgaaa
     2401 ttttttacat ttttacacgt cagcgcggcc gacacgcttg taacacactg taacatcttg
     2461 ggttcttctg ctagcattgg aattgctaca cgcagaacgc cagattggga ggcgtggttt
     2521 ttgatgcgaa acggcacacg ggcgtggagg cggcgggccc ctttccatcg cgggggagtc
     2581 gtcgcgatcc tggccgccac ctgcatgctg gcgggtggga ccgcagcccg
//
LOCUS       DASSZH010000248         3314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_39672, whole genome shotgun sequence.
ACCESSION   DASSZH010000248 DASSZH010000000
VERSION     DASSZH010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39672"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..1872
                     /gene="serA"
                     /locus_tag="VFN42_05285"
     CDS             286..1872
                     /gene="serA"
                     /locus_tag="VFN42_05285"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HET8996062.1"
                     /translation="MPKVLISDKLSPAAVEIFRRRGIEVDVKPGLSPADLRAIIAAYD
                     GLAIRSATKVTRELLDAATNLKVVGRAGIGVDNVDVKSATARGVVVMNTPHGNTITTA
                     EHAIAMMFALARQIPEASASTKAGKWEKNRFMGVELSGKTLGLIGCGNIGSIVADRAV
                     GLKMKVVAYDPYLSEKRALALGVEKAELDELLARADFITLHTPLTDATRNILSREALA
                     KTRPGVRIINCARGGLVDEVALADAIRSGHVAGAALDVFETEPAKDSPLFGLENVVCT
                     PHLGAATQEAQENVALQVAEQMSDFLLSGAVANAINMPSVSAEDAPRLKPYMERCRLL
                     GAFAGQLTQAREGSIRRVIIEYEGQAAALNTRPLSAAVLAGLMSPLMEGINMVNAGIA
                     AREHGIDVAETVHDRPSEYQTLVRVTVETDKLSRSVAGTLFAGSRPRLVEIKGIKVEA
                     DFAPHMLYVTNQDKPGFIGRFGATLAGAGINIATFHLGRAAQGGDAICLVSVDDSVPE
                     DVLAMVRTLPLVVQATALSF"
     gene            2072..3304
                     /locus_tag="VFN42_05290"
     CDS             2072..3304
                     /locus_tag="VFN42_05290"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HET8996063.1"
                     /translation="MDTGGPLILVINSGSSSLKCAVFAGEHRAPLLTGLAERLGQAQA
                     TIAFKPDGTAATLPEPTHEAALDAVLAALTGRDWLPRLAAVGHRVVHGGEHFAQSVVI
                     TPEVIADIDACSRLAPLHNPPALRGIRSAMQRLPHVPHVAVLDTAFHQTMPPAAYYYA
                     LPMDLYRQLGVRRYGFHGTSHRYVAAEAVRLLGLDPQDHGLVTAHLGNGASATAVQDG
                     RSVDTTMGMTPLEGLVMGTRCGDIDAGALSLIARRTGWDWDALDDVLNKRSGLLGLSE
                     LSNDCRELEQAAQSGHAGARLALEVFVHRLARHIGGLAMALRRLDAVVFTGGIGENSA
                     WMRAATLQRLGPLGLALDAAANARMTGGAAGRISRGSAPAALVVPTNEEWMIARDAAA
                     LAGLHAPSSADSGSPNAS"
BASE COUNT          535 a         1139 c         1133 g          507 t
ORIGIN      
        1 gcatccgcat ctggggcggt gccacggtgg agccgcgcga cgtgcaggcg ctgctgccct
       61 ggttcgactg ggcgcatgag gtcgttgcac gcgagtatgc agagccaaag gctgcctagt
      121 tcacccacag cacccccccg cgatgtcatg accggcgaag gccgggcacc cacgtcttgt
      181 tccgatagct gcggcaaaga cgtgggtgcc gggccgagcc aggccatgac accaacagcc
      241 cggcaacgcg ggcggggggc atgactcaca gaccagagaa atcccatgcc caaggtcctg
      301 atcagcgaca agctgtcccc cgccgccgtg gagatctttc gccgccgcgg catcgaggtc
      361 gatgtgaaac ccggcctgtc gcccgccgat ctgcgcgcca tcatcgcagc ctatgacggg
      421 ttggcgatcc gctccgccac caaggtcacg cgcgaactgc tggacgccgc gaccaacctg
      481 aaagtggttg gccgcgccgg catcggcgtg gacaatgtcg atgtgaaatc cgccaccgcg
      541 cgcggcgtgg tggtgatgaa cacgccgcac ggcaacacca tcaccaccgc ggagcacgcc
      601 atcgccatga tgttcgcgct ggcgcggcag attcccgaag cctctgcttc caccaaggcc
      661 ggcaagtggg agaagaaccg cttcatgggc gtcgagctgt ccggcaagac gctgggcctg
      721 atcggttgcg gcaatatcgg ctcgatcgtc gccgatcgtg cggtcggcct gaagatgaag
      781 gtggtggcct acgatcccta tctgtcggaa aagcgcgcac tcgccctggg cgtggaaaag
      841 gccgaactgg acgagctgct cgcgcgtgcc gatttcatca cgttgcacac gccgctgacc
      901 gacgccacgc gcaacatcct gtcgcgcgag gcgctggcga agacccgtcc tggcgtgcgc
      961 atcatcaact gcgcccgcgg cggcctggtg gacgaagtgg ccctggcgga cgcgatcagg
     1021 tccggccacg tcgccggcgc cgcgctggac gtattcgaga ccgagccagc gaaagattcg
     1081 ccgctgttcg ggctggagaa tgtggtgtgc accccgcatc tcggcgccgc cacccaggag
     1141 gcgcaggaga acgtggcgct gcaggtcgcc gaacagatga gcgatttcct gctgagcggg
     1201 gcggtggcca atgcgatcaa catgccgtcc gtctcggccg aggatgcgcc gcgcctgaag
     1261 ccctacatgg aacggtgccg cctgctcggc gcctttgccg gacagttgac gcaggcgcgc
     1321 gagggcagca tccgccgcgt catcatcgaa tacgagggcc aggccgcggc actgaacacc
     1381 cgcccgctca gcgcggcggt gctggccgga ctgatgtcgc cgctgatgga gggcatcaac
     1441 atggtcaatg ccggcattgc cgcgcgcgag cacggcattg acgtggcgga gaccgtgcac
     1501 gaccggccga gcgagtatca gacgctggtg cgggtgacgg ttgagacgga caagctgtcg
     1561 cggtccgttg ccggcacgct gttcgccgga tcgcgtccgc gcctggtgga gatcaagggc
     1621 atcaaggtgg aggccgattt cgccccgcac atgctgtacg tcaccaacca ggacaagccg
     1681 ggtttcatcg gccggttcgg tgcgacgctg gcgggggcgg ggatcaacat cgccaccttc
     1741 caccttggcc gcgcggcgca gggcggcgac gcgatctgcc tggtgtcggt ggatgattcg
     1801 gtgccggagg acgtgctggc gatggtccgc accctgccac tggtggtgca ggcgaccgcg
     1861 ctgtcgttct gacggcagga ggcattccat tctccccctc ccacaagggg agggggaggg
     1921 gtctaccctc caaccggccc gctgcttgat cgagatcaac ccgctgttaa cccaggcccg
     1981 gtattccgtg ccctcacgct aggccgccat tgcggccgga cgctcttccg tccggccgtg
     2041 ctcgcggctg acccatgtgc gagggcaacg gatggatacc ggcgggccat tgatcctggt
     2101 gatcaacagc ggctcctctt cgctgaaatg cgcggtcttt gccggggaac accgggcacc
     2161 gctgctgacc ggcctcgccg aacggcttgg ccaggcgcag gccaccatcg ccttcaagcc
     2221 ggacggcacc gcagccacgc tgcccgagcc gacgcatgag gccgcgctgg acgctgtgct
     2281 ggcggcgctg accggacgcg actggctgcc gcgcctggcg gcggtgggac accgcgtggt
     2341 ccatggcggc gagcacttcg cgcagtcggt ggtgatcacg ccggaggtga tcgcggacat
     2401 cgacgcctgc tcccgcctcg cgccgttgca caatccaccc gcactgcgcg gcatccgctc
     2461 cgccatgcaa cgcctgccgc acgtgccgca tgtcgcggtg ctggacaccg cattccatca
     2521 gaccatgccg ccggcagcct actattacgc cctccccatg gacctgtacc gccaactcgg
     2581 cgtgcgccgc tacggcttcc acggcaccag ccaccgctac gtcgccgccg aggctgtgcg
     2641 cctgctcggc ctggacccgc aggaccacgg gctggtgact gcgcatctgg gcaacggcgc
     2701 ctccgccacc gcggtgcagg acgggcgcag cgtcgatacc accatgggca tgaccccgct
     2761 ggagggtctg gtgatgggca cgcgctgcgg cgacatcgat gccggtgcgc tgtcgctgat
     2821 cgcccgccgc accggctggg actgggatgc gctggacgac gttttgaaca agcgcagcgg
     2881 cctgctgggt ctttccgaac tatcgaacga ctgccgggaa ctggaacagg cggcgcaatc
     2941 cggccacgcc ggcgcgcgac tggcgctgga ggtgttcgtg caccggctcg cccgccacat
     3001 cggcggcctg gcgatggcat tgcgaaggtt ggatgcggtc gtgttcaccg gcggcatcgg
     3061 cgagaactcc gcctggatgc gcgctgccac gctgcaacgc ctcggcccgc ttggcctggc
     3121 gctggacgcc gccgcgaacg cgcgcatgac cggcggagcg gcaggccgga tcagccgcgg
     3181 ctccgcgccg gccgcgctgg tcgtgccgac aaacgaggaa tggatgatcg cgcgcgatgc
     3241 cgccgcgctc gccggactgc atgcaccctc ctccgccgat agcgggagcc cgaacgcgtc
     3301 atgaatgccg cctg
//
LOCUS       DASSZH010000249        12302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1216004, whole genome shotgun sequence.
ACCESSION   DASSZH010000249 DASSZH010000000
VERSION     DASSZH010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 12302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12302
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216004"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2035)
                     /locus_tag="VFN42_05295"
     CDS             complement(<1..2035)
                     /locus_tag="VFN42_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HET8996064.1"
                     /translation="MTAIPGDSRLADRLRRNLRGAVLFSPADRGRYATDASIYQVEPL
                     GVVVPETTDDVAAALTIARDEGIPVLPRGGGTSQCGQTVNRALVLDCSKHLRRILHID
                     PQAQTALVEPGVVLGQINTALRAENLFFPVDPSTHARCTIGGMAGNNSCGSKSIRYGL
                     MADNVRAIDAILADGTRHRFGAVPDNLGADVPANVAELIQRLRSLGAREAEEIAARFP
                     RQLRRVGGYNIDVLTPSARSAGRENLARLLVGSEGTLAFSAALELSLHPLKPRKLLGI
                     CQFSTFRAAMSAAQHIVQLDPEAVELVDRTMIDLGRSIPIFRATIDRMLLGEPDSLLI
                     VEFHGWEDAPLLARLAELDTLMSDLGHPGQVVRALDPTFQADIASVREAGLNIMMSMK
                     GDGKPVSFIEDCAVALEDLADYTERLNGVFEKHGTKGTWYAHASVGCLHVRPVLNMKD
                     PADVAKMRAVAEEAFALVREYKGSHSGEHGDGIVRSEFNETMFGSRIARAFEAVKDAF
                     DPNNLLNPNRVVRPPRMDDRSLFRYAPGYAAAPDFVPKLDWSEHSGPLRGMLGAVEMC
                     NNNGTCRAFDAGVMCPSFRVTRDEQHLTRGRANTLRLALTGQLGPDAMTSDAVADAMR
                     FCVSCKACRRECPTGVDMAKMKLEVLSARAERYGTSRRESLIANLPRYAPMMSR"
     gene            complement(2167..3129)
                     /gene="cysK"
                     /locus_tag="VFN42_05300"
     CDS             complement(2167..3129)
                     /gene="cysK"
                     /locus_tag="VFN42_05300"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET8996065.1"
                     /translation="MPIVLDRPKRGRIYDSIAETIGNTPLVRIPNITVEDGIKADICL
                     KLEFFNPIASVKDRIGVNMILAMEAEGKLKPGTTIIEPTSGNTGIALAFVAAARGYKL
                     ILTMPESMSIERRKMLAYLGAQLELTPREKGVNGAIARAKELQASIPGSVIAGQFDNP
                     ANPDIHRRTTAEEIWYDTAGEVDVIVSGIGTGGTLTGCAQALKPRRPGLRMVAVEPSA
                     SAVLSGGQPGPHMIQGLGAGFVPNVLDRDAIDEVIAVSNEDAIAHSRRLARSEGIPCG
                     ISSGAALCATLQVAKRPEMAGKRIVTIIPSFGERYITTALFEGL"
     gene            3473..4648
                     /locus_tag="VFN42_05305"
     CDS             3473..4648
                     /locus_tag="VFN42_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET8996066.1"
                     /translation="MSQSRHAGRHFLQIPGPTNVPDRVLRAIDNPTIDHRGPEFGVMG
                     RELLTNLKAVFQTSGPVIVYPASGTGAWEAALTNTLSPGDTVLMCRTGWFATLWKEMA
                     ERLQLNPIFIDTDWRRGADVDALGSALAEDKAHRIKAVCVVHNETSTGCTSRIDEVRA
                     AIDAANHPALLLVDTISSLASIDYRHDEWGVDVTVAGSQKGLMLPPGLSFNAVSEKAL
                     KAAQTAKLPRSYWDWRPMLEANKTGYFPYTPGTNLLFGLNEAVKMLLDEGLANVFARH
                     DRHAEATRRAVRSWGLEIQCAEPRHFSSSLTAVRLPEGHSADALRATILDKYNMSLGN
                     GLGMLKDRVFRIGHLGDFGDLQLIGTLGGVEMGLRVAGVPHKQGGVQAAVDYLSGNA"
     gene            complement(4657..6189)
                     /locus_tag="VFN42_05310"
     CDS             complement(4657..6189)
                     /locus_tag="VFN42_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator"
                     /protein_id="HET8996067.1"
                     /translation="MNTPRLDRRRALALAGAAWVGSSTQAIAQHPAASPHESPAKPPA
                     AENRPKLPPPVTTQHVLTLPNRVLHFTATAGAIPLTSGEKHTPLADIAFVAYQRDGTD
                     RDQRPVTFVFNGGPGFASAWLQLGAVGPWRIPLGTQSTSPSATPVPSPNSDTWLDFTD
                     LVFIDPAGTGYSQALVSGEQARQHLWSVDGDITYLAEAIRRWIDRYNRMLSPKYILGE
                     SYGGFRAPRLAYELATKHGTGVRGLALVSPALEIISPNSTFEPFYYVTRLPSMAAAAR
                     GRHGAVTRAQLADVEAYAATDYLVDVVRGERDAAAIDRRSARVAAFTGLDPTLVRRHH
                     GLIDNDVFLHALYRSQARVGSVYDATISDADPYPLETLSSAPDPILDGLQAPVSSAMV
                     AVYENKLRWRPETTYRLGNLVIARQWDWGQKFGGRPESLHALREALALDGNLSVLIGQ
                     GMFDLVVPYFQTQLILDQIPESVGMNRVRFTVHNGGHMFYMDDPARAALREDAAALIG
                     PG"
     gene            complement(6330..6713)
                     /locus_tag="VFN42_05315"
     CDS             complement(6330..6713)
                     /locus_tag="VFN42_05315"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET8996068.1"
                     /translation="MSAISRMLLDDQPAPVSHYCHVVRAGNLVWVSGTVGMNAAGQIP
                     DDVVEQFDLVVQNLDACLKAAGAGPQHVVKVTVYLTDIDDRARINPARQRYFGEHRPA
                     STLVEVSALVLPALKVEMEAQAIIA"
     gene            6919..7692
                     /locus_tag="VFN42_05320"
     CDS             6919..7692
                     /locus_tag="VFN42_05320"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8996069.1"
                     /translation="MAYSPFDLTGQVALVTGGNSGIGLGMARALAQAGADIAIWGTNP
                     SKNAQAKAELDTTGRRVLALECDVGDEMAVEQAFATTVTEFGRVDGCFANAGVSGRGV
                     ASFLEMSNAEWHRVTRVNLDGAFYTFRTAAKHMVQRGGGGVLVGTASLAAVEAAPRGE
                     HYAATKGGMISMLRALAVEFARHGIRAHTILPGWIETNMTERTIANPTFSGKVMPRIP
                     MRRWGTGDDFGGIAVYLMSSAARYHTGDTFVIDGGYSLF"
     gene            complement(7770..9113)
                     /locus_tag="VFN42_05325"
     CDS             complement(7770..9113)
                     /locus_tag="VFN42_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996070.1"
                     /translation="MTSAADAINAALQNATTIVGSTVTDPSSIPAAPSVPAGGASELV
                     INTTATGAISIPAGYQYVVINDPNPVTITGSDAVIINSNTTGGSSFSVTGNSTVLAAG
                     GNNTFNLGTGSANGSTYSMTSGPGDDTVYAGGSGTIQPGGGTNVVVDPGNNLIVSTGT
                     NDLIVGSSGVTSIQASGPGAQILGASGDTMYVTMTGSNGIAAANGATVSVNLGGSGNT
                     LVGGSAAETVMASTGGLMFGGSGNITFVGGSTASTLVGGANGSEAVSVGSGGVLFAAS
                     SGNAGTVTSGTGTATLFGSSNTTITFNGSLQGAIYVAGSGNETLNASGGSTNNIIVGG
                     ADTAGANSLVGGTGNDTIFAGGGSDTMVTGNGNDAAVFFKSNTHGAHNFITDFNPSND
                     TLWLLGYDSTKSASNLVDNASTVGGGVTLTLSDNTKITFTNLTSSSQLYGKIFYG"
     gene            complement(9970..10389)
                     /locus_tag="VFN42_05330"
     CDS             complement(9970..10389)
                     /locus_tag="VFN42_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET8996071.1"
                     /translation="MNREMSEIIRNQRPLTLPPSATVQEACRHMHQRRIGAVLVTDPG
                     CRLIGIFTGRDAVRVLAEGKDPVSATLDGVMTREPACMPPRSTAIDALRLMRDGGFRH
                     VPVVQDGRVAGIVSRGDFRGLEQDRLDEETGIWERMR"
     gene            complement(10459..11493)
                     /gene="pdxA"
                     /locus_tag="VFN42_05335"
     CDS             complement(10459..11493)
                     /gene="pdxA"
                     /locus_tag="VFN42_05335"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HET8996072.1"
                     /translation="MPANPPRLAITMGDPAGVGPEIIVKACARLMPRLQSGEMRLLVI
                     GHRSALRASCDLLQSGLSFPETGRDADFPPLALLPAGEELAPIRFGELTPEAGRFAYL
                     AVETAVALAQAGRIDAIVTAPLNKEALNRAGYHYAGHTDMLAALTHTRSSVMLLAHGD
                     MRVSHVTTHVALEKVPALLTPERLRRTIDLTHAALLDLGIARPRIAVAALNPHAGEGG
                     LFGTQDITVTTPVVAQCLAEGLAVTGPVPGDTVFVKLRARQFDAVVAMYHDQGHIPVK
                     LLGFQVDPATGAWQALSGVNVTLGLPVIRTSVDHGTAFDIAGKGVANEDSLLEAVDYA
                     LRLASNRTAV"
     gene            complement(11615..12301)
                     /locus_tag="VFN42_05340"
     CDS             complement(11615..12301)
                     /locus_tag="VFN42_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010503818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET8996073.1"
                     /translation="MFKRRKPDEMPAGSGGTLPAGLSATDSPSRFAEEAGKTDLHPRT
                     PGAASGPADSGLGVPPFRPAQPKEAQSMSRPPFPATPGSPSAAPGQPARPAAAPSRTA
                     NRDPGERRTLVVGRGISVQGTVQDAERLVVEGTVEASMIHATELAIAPGGVFKGEVEV
                     EDAEIAGTIDGTLTARGSLVVRATGRVLGTARCRRLQVEDGGQITGRIEMLGDQVRTE
                     APRLAAEPAD"
BASE COUNT         2031 a         4082 c         4139 g         2050 t
ORIGIN      
        1 tgcgcgacat catcggcgcg tagcgcggca ggttggcgat cagcgattca cgccggctgg
       61 tcccgtaccg ttcggcgcgt gccgacagca cctcgagctt catcttcgcc atatcgacgc
      121 ccgtcgggca ttcacggcga cacgccttgc acgacacgca gaaccgcatc gcatccgcaa
      181 ccgcgtccga cgtcatcgcg tccgggccaa gctgcccggt cagcgccaga cgcagcgtgt
      241 tggcgcggcc gcgcgtcaga tgctgttcgt cgcgcgtcac ccggaaggac gggcacatca
      301 cgccggcatc gaaggcgcgg caggtaccgt tgttgttgca catctccacc gcgccaagca
      361 tgccgcgcag cggccctgaa tgctcggacc agtcgagttt cgggacgaag tcgggggcag
      421 ccgcgtagcc gggcgcgtag cgaaacagcg accggtcgtc catgcgcggc ggacgtacca
      481 cgcggttcgg gttcagcagg ttgttcggat cgaacgcgtc cttcaccgcc tcgaaggcac
      541 gcgcgatgcg gctgccgaac atcgtctcat tgaactcgct gcgcacgatg ccgtcgccgt
      601 gctcgccgct gtgcgagcct ttgtactcgc gcaccagggc gaaggcttcc tccgcgaccg
      661 cgcgcatttt ggcgacatcc gccgggtcct tcatgttcag caccggccgc acgtgcaggc
      721 agccgaccga agcatgcgcg taccacgtgc ccttggtccc gtgcttttcg aacacgccgt
      781 tcaggcgttc ggtgtagtcc gccaggtctt ccagggcgac ggcacagtcc tcgatgaagg
      841 acaccggttt gccgtcgccc ttcattgaca tcatgatgtt caggccggct tcgcgcacgc
      901 tggcgatgtc ggcctgaaag gtgggatcga gcgcgcgcac gacctggccg ggatggccca
      961 gatcgctcat cagcgtgtcc agctcggcca gcctggccag cagcggtgcg tcttcccagc
     1021 cgtggaattc gacgatcagc agcgaatcag gctcgcccag cagcatgcgg tcgatcgtgg
     1081 cgcggaagat cggaatgctg cggcccaggt cgatcatggt gcgatcgacc agctccaccg
     1141 cttccggatc gagctgcacg atatgctgcg cggcagacat ggcggcgcgg aaggtggaga
     1201 actggcagat gcccagcagc ttgcgcggct tcagtggatg aagcgagagc tccagggcgg
     1261 cggagaaggc gagggtgccc tccgatccca ccagcagccg cgccaggttt tcgcgtccgg
     1321 cggaccgcgc ggatggggta agcacgtcga tgttgtagcc gccgacacgc cgcaattgcc
     1381 gcggaaagcg cgcggcgatc tcctccgcct cgcgcgcacc gagtgaacgc aggcgctgga
     1441 tcagctcggc cacattggcc ggcacgtccg cgccgagatt gtccggaacc gcgccgaaac
     1501 gatggcgcgt gccgtcggcc aggatggcgt cgatggcgcg cacattgtcg gccatcaggc
     1561 cgtagcggat cgatttggag ccacaggagt tgttgccggc catgccgccg atggtgcagc
     1621 gcgcgtgcgt ggacggatcg accggaaaga acaggttttc ggcgcgcaac gcggtgttga
     1681 tctggccgag caccacgccc ggctccacca gcgcggtctg tgcttgcggg tcgatgtgca
     1741 ggatgcggcg caggtgcttg gaacagtcca gcaccagcgc gcggttcacc gtctggccgc
     1801 actggctggt gccaccgccg cgcggcagca cggggatgcc ctcatcacgc gcgatcgtca
     1861 gcgcggcggc aacgtcatcg gtggtttccg gcacgacgac gcccagcggt tcgacctggt
     1921 agatcgaggc gtcagtggcg tagcggccgc ggtcggcggg cgagaacagc actgcgccgc
     1981 ggagattgcg gcgcaggcgg tcggccaggc gggagtcgcc ggggatggcg gtcatgcgag
     2041 ctgtccgggg ctcctccgtg tcatggccgg gcctggcccg gccacccacg tcctatcatg
     2101 ttccatgcgc caaagacgcg ggtggccggc ctgcaccggc catgacacgg tgaggcctgc
     2161 ccgcacttac agcccctcga acagcgccgt ggtgatgtag cgctcgccga aggacgggat
     2221 gatggtgacg atgcgcttgc ccgccatctc cgggcgtttc gccacctgca gcgtggcgca
     2281 cagcgcggcg ccggaggata tgccgcacgg gataccctcg ctgcgagcaa ggcggcggga
     2341 atgcgcgatg gcgtcctcgt tcgacaccgc tatcacctcg tcgatcgcat cgcggtccag
     2401 cacgttggga acgaagccgg cgccgagccc ctggatcatg tgtgggccgg gctggccgcc
     2461 ggacagcacg gcgctggccg agggctccac cgccaccatg cgcaatcccg gccgccgagg
     2521 cttcagtgcc tgcgcgcagc cggtcaacgt gccgccggtg ccgatgccgg aaacgatcac
     2581 gtccacctcg cccgcggtat cgtaccagat ctcctccgct gtggtacggc ggtgaatgtc
     2641 agggttggct ggattgtcga actgcccggc gatcaccgaa ccagggatcg acgcctgcaa
     2701 ttccttcgcg cgcgcgatag cgccgttcac ccctttctcg cgcggcgtca actccaattg
     2761 tgcgccgaga taggcaagca tcttacgccg ctcgatcgac atggactcag gcatggtcag
     2821 gatcagcttg tagccgcgcg cggcggcgac aaaggccagc gcgatgccgg tgttgccgga
     2881 agtcggctcg atgatcgtgg tgccaggctt cagcttgccc tcggcttcca tcgccaggat
     2941 catgttgacg ccgatgcggt ccttcacgct ggcgatcggg ttgaagaact ccagcttcag
     3001 gcagatgtcc gccttgatgc cgtcctcaac tgtgatgttc gggatgcgca ccagcggcgt
     3061 gttgccgatg gtttccgcga tgctgtcgta gatgcgtccg cgcttcggac gatcgagtac
     3121 gatgggcata gttccgtcgg tccgcgccat aggtcctctc ccgtagcttt ttttccagcg
     3181 cgtacctaac atgccgggtg tccatggcca atgcaggcac gcctgcccac tattggaccg
     3241 tttccgcaaa atcccgccga acgacgcaca aggaggaagt taccggcgga atacggctcg
     3301 ctgcagcaaa tcaaagcccg ccgggagcgc cgcaggcgaa gtttttctcc tctgcttggc
     3361 gcgacaggac gcgctgatgc cggtcgcaag ccgcagcgga tggcgcgaga cgccgttggc
     3421 aattcgccca atcacgtcca aggtgcgcgc ctcgccccaa cggaggtcct gcatgagtca
     3481 atcccgccac gccggccggc atttcctgca aatccccggc ccgaccaatg tgccggaccg
     3541 cgtgctgcgc gccatcgaca acccgaccat cgatcaccgc ggtccggaat tcggtgtcat
     3601 gggccgcgaa cttttgacca atctcaaggc ggtgttccag accagcggcc cggtgatcgt
     3661 ctatccggcc tccggcaccg gtgcgtggga agcggcgctg accaacacgc tgtcgcccgg
     3721 cgacaccgtg ttgatgtgcc gcaccggctg gttcgccacg ctgtggaagg agatggcgga
     3781 gcggctgcag ctcaacccga tcttcatcga caccgactgg cgccgcggcg cggacgtgga
     3841 tgcgctcggc agtgccctgg cggaagacaa ggcgcatcgc atcaaggcgg tctgcgtggt
     3901 gcacaacgaa acctccaccg gctgcaccag ccgcatcgac gaagtgcgcg cggcgatcga
     3961 tgccgccaac cacccggccc tgctgctggt ggataccatc tcgtcgctgg cctccatcga
     4021 ctatcggcac gacgaatggg gcgtcgatgt caccgtggcc ggttcgcaga aggggctgat
     4081 gctgccgccg ggcctgtcgt tcaatgcggt gagcgagaag gcgctgaagg cggcgcaaac
     4141 cgccaaattg ccacgcagct actgggattg gcgcccgatg ctggaggcga acaagaccgg
     4201 ctacttcccc tatacccccg gcaccaacct gctgttcggc ctgaacgagg ccgtgaagat
     4261 gctgctggac gaaggcctgg ccaacgtgtt cgcccggcat gaccgccacg ccgaagccac
     4321 ccgccgtgcg gtgcgaagct ggggcctgga gatccagtgc gccgaaccgc gccatttctc
     4381 gtcgtcgctg actgcagtac gtctgccgga ggggcacagc gccgacgcgc tgcgcgccac
     4441 catcctggac aagtacaaca tgagcctggg gaatggcctg ggcatgctga aggaccgcgt
     4501 gttccgcatc ggccatctcg gcgatttcgg cgacctgcag ttgatcggca cgctgggtgg
     4561 cgtggagatg gggctacgcg tcgccggcgt gccgcacaaa cagggcggcg tccaggccgc
     4621 cgtggattac ctgtccggca acgcctgacg ccggattcag cctgggccga tcagcgccgc
     4681 ggcatcctca cgcaatgccg cgcgcgcagg atcgtccatg tagaacatgt gccccccgtt
     4741 gtgcacggtg aagcgtacgc ggttcatgcc gaccgattca ggaatctgat cgaggatcaa
     4801 ctgcgtctgg aaatacggca cgacgaggtc gaacatcccc tggccgatca gcaccgacag
     4861 atttccatcc agcgccaacg cctcgcgcaa agcgtgcagc gactccggcc ggccgccgaa
     4921 tttctggccc cagtcccatt gccgtgcgat caccagattg cccagccgat aggtcgtctc
     4981 cggccgccac cgcagcttgt tctcatagac ggccaccatc gcgctgctca ccggcgcctg
     5041 cagcccgtcc aggatcgggt cgggggcact gctcagggtt tccagcggat acggatcggc
     5101 atcgctgatg gtggcgtcgt acacgctgcc gaccctcgcc tgtgagcggt acagcgcgtg
     5161 caggaacacg tcattgtcga tcagcccgtg atggcgccgc accagcgtcg gatcgaggcc
     5221 ggtgaacgcg gccacccgcg cactgcgccg gtcgatcgcc gcggcgtcgc gttcgccgcg
     5281 caccacatcc acgaggtaat ccgtagcagc ataagcttcg acatcggcaa gctgcgcccg
     5341 cgtcacggct ccatgccgcc cacgcgccgc tgccgccatc gagggcaggc gtgtgacata
     5401 gtagaacggc tcgaaggtgc tgtttggcga tatgatctcc agcgctggcg agaccagcgc
     5461 caggccgcgc acgccggtgc cgtgcttggt ggcgagttcg tacgccagcc gcggagcgcg
     5521 gaacccgccg tagctctcgc ccaggatgta tttcggcgac agcatacggt tgtagcgatc
     5581 gatccagcgg cggatcgcct ccgccagata ggtgatatcg ccgtccaccg accacaggtg
     5641 ctgccgcgcc tgctcgccgc tgaccagcgc ctgggaatag ccggtgccgg ccgggtcgat
     5701 gaacaccaga tcggtaaagt cgagccacgt atccgaattc gggctcggca ccggtgttgc
     5761 ggacggggaa gtactctggg tgccaagcgg aatccgccac gggccaaccg cgccaagctg
     5821 cagccatgcg gaggcgaaac ccggaccccc gttgaagacg aaggtgaccg gccgctgatc
     5881 ccgatccgtg ccgtcgcgct ggtaggcgac gaaggcgatg tccgccagcg gcgtgtgctt
     5941 ctcgccgctg gtcaacggga tcgcgccggc cgtggcagtg aaatgcagca cccgattggg
     6001 cagtgtcagc acgtgttggg tggtgacagg cggcgggagt tttggccggt tttcggcggc
     6061 cggtggcttc gccggtgatt catgcggtga cgccgccgga tgctgcgcga tcgcctgggt
     6121 cgagctgccg acccaggcgg ctccggcaag cgcaagcgcg cgccggcgat ccaggcgggg
     6181 agtgttcatc ggcgcagtac ctctgaggtg acaaccggcg gccggcgcaa cccagcacca
     6241 gcgctgcggc aatgcaatcg tcagccggtc atgaggcttc acaagggagt tcgctcccgc
     6301 gttcggtacg ggacggaacg cgctcctggt caggcgatga tcgcctgcgc ctccatttcc
     6361 actttcagtg caggaagcac cagggcggaa acctccacca gcgtcgatgc cgggcgatgt
     6421 tcgccgaagt aacgctgccg agcgggattg atgcgcgcgc ggtcgtcgat atcggtcaga
     6481 tagacggtca ccttcaccac gtgctgcggc ccggcgcccg ccgccttgag gcaggcatcg
     6541 agattctgca ccacgagatc gaattgctcg acgacgtcat ccggaatctg gcccgcggca
     6601 ttcatgccca ccgtgccgga tacccagacc aggtttccgg cgcgcacgac atggcagtaa
     6661 tggctgaccg gggcaggctg gtcgtccagg agcatgcggc tgatcgcgga catcggcttc
     6721 ctccatcagg atggtcttgt cgccagctta gccacaatcc gaggatgcgc ggcgtgccat
     6781 ttcgaccaga tcgcaatgtc gccgcggcgc tgagtcccgg gccagcacag atgatggcgg
     6841 gtgacccagg acgcggcttc gcatgccgac atgggccggc taccctgcca tcccaggacg
     6901 caaagcggag gaacgcaggt ggcctacagc ccattcgatc tgaccggcca ggtcgcgctg
     6961 gtgaccggcg gcaacagcgg cattggcctc ggcatggcgc gtgccctggc gcaggccggg
     7021 gccgatatcg cgatctgggg caccaacccc agcaagaatg cccaggccaa ggcggagctg
     7081 gacaccaccg gacggcgggt tctggcgctg gagtgcgacg tgggcgacga aatggcggtg
     7141 gagcaggcat ttgccaccac cgtgaccgag ttcggccgcg tggacggctg cttcgctaat
     7201 gccggggtgt ccggccgtgg cgtggcatcc ttcctggaga tgagcaatgc ggaatggcat
     7261 cgcgtcaccc gggtaaatct cgacggcgcc ttctatacgt tccgtaccgc cgcgaagcac
     7321 atggtgcagc gaggcggcgg cggggtgctg gtcggcacgg cctcgctcgc cgcggtggaa
     7381 gcggcgccgc gcggcgagca ctacgcagcc accaaaggcg gcatgatctc catgctccgc
     7441 gcgctggcgg ttgagttcgc ccgccatggc attcgcgcgc acaccatcct gcccggctgg
     7501 atcgagacca acatgacgga gcggacgatc gccaacccca ccttcagcgg caaggtcatg
     7561 ccgcgcattc cgatgcggcg ctggggcacc ggcgacgatt tcggcgggat cgcggtgtac
     7621 ctgatgagct cggcggcgcg ctaccatacc ggcgatacgt tcgtgatcga cggcgggtat
     7681 tcgctgttct gaagaactgc ggcggccgaa atgcaaagtg ccgagggggg caccctcggc
     7741 actggcaggc ttcggcgctg gaagccggat cagccgtaga agatcttgcc gtagagctgg
     7801 ctggagctgg tcaggttggt gaaggtgatc ttggtgttat ccgacagggt cagggtcaca
     7861 ccaccgccca ccgtgctggc attgtccacc aggttggagg ctgacttggt cgagtcgtag
     7921 ccgagcagcc acagcgtgtc gttgctgggg ttgaagtcgg tgatgaagtt gtgggcgccg
     7981 tgggtgttgc tcttgaagaa gaccgcggcg tcgttgccgt tgccggtcac catggtatcg
     8041 ctgccgccgc cggcgaagat ggtgtcgttg ccggtgccgc ccacgaggga gttcgcccct
     8101 gcggtatcgg cgccgccgac gatgatgttg ttggtcgagc cgccggacgc attgagcgtc
     8161 tcattgccgc tgcctgccac gtagatcgcg ccctgcagcg agccgttgaa ggtaatggtg
     8221 gtgttgctgg agccgaacag cgtggcggta ccggtgccgc tggtcacggt tcccgcgttg
     8281 ccggagctgg ccgcgaacaa cacgccgccg ctgccgaccg aaaccgcctc actgccattg
     8341 gcgccgccca ccagcgtcga ggcggtgctg ccgccgacga aggtaatgtt gccggagccg
     8401 ccgaacatca ggccaccggt ggaggccatg acggtttccg ccgccgagcc gccgaccagg
     8461 gtgttgccgg aaccgcccag attgaccgac acggtggcgc cgttcgccgc ggcgatgcca
     8521 ttgctgccgg tcatcgtaac atacatggtg tcgccggaag cgccgaggat ctgcgcgccg
     8581 ggtccgctcg cctggatcga ggtgacgccg gacgagccga cgatcaggtc gttggtgccg
     8641 gtcgagacga tcaggttgtt gccggggtcg accacgacgt tggtgccgcc gccgggctgt
     8701 atggtgcccg aaccgccggc atacaccgtg tcgtcacccg ggccgctggt cattgaatag
     8761 gtggacccgt tggccgaccc ggtgccgaga ttgaaagtgt tgttgccgcc ggccgccagc
     8821 accgtgctgt taccggtcac gctgaagctg ctgccaccgg tcgtgttgct gttaatgatg
     8881 accgcgtcgc tgccggtgat ggtgaccggg ttggggtcgt tgatcaccac gtattggtag
     8941 ccagcgggga tcgaaatggc gccggtggcg gtcgtattga tgaccagctc agatgcgccc
     9001 ccggcgggca cggaaggtgc ggcaggaatt gacgagggat cggtcacagt gctgccgacg
     9061 atggtcgtgg cattctgcag cgcagcattg atcgcgtccg cggcgcttgt cacgccggca
     9121 ctcgtccccg ggatcgtggc aactggaccg cccccagggc ctggaatcgt agtagccatt
     9181 cctcgtctcc gtctgtcagg gcactggcgc gagctgccgg ggccaacgaa ttatggatta
     9241 gggtcaactt tttgcaacaa atttcgcgcg tggcgaccca tcgcaaaagc cgacccccag
     9301 ccgttggggc taaaccagat gatatagcgc cgatcgggga acgcctcaaa acatttcgtt
     9361 accgcggcac gcaaaaactg cccgggtgca agcccactga tgccgggatt tcaaacagat
     9421 gacgatgcga tggtatgcag atcgagtgca accgagccgg tttcctccgt tgtccaagtc
     9481 agggccgtcc tcctcagacg attccatagt actcccgatg actgctttct cggtcgagaa
     9541 gatcgccgct ctttctggtg acaaatgcgg gccattccac cacaggaagg tggcgctata
     9601 tccgacctac aacgatacca cgtgagaccg attgcctcgt gatattattc ggccacctca
     9661 atacgtcgtc agataattcg acactccggc ggaattttcc tgaaggtgcc gggtgggtgc
     9721 cggcccccgc ggtcgaaaaa cgcgtcattc gccatggctt cacgttgcac ggagcggcgc
     9781 cgaagcgcga gcgaccttgc catctgaccg aagacggtcc ccggtttcgt atatgttccc
     9841 gagactcgac cggggcaccg ccaggggcaa agcgcaagcc cggcatcggc tcgccccgtg
     9901 tcctcagcgg cagccggccc agccgccaat cgccgccggt gggttgcagg cacccgcgcg
     9961 ccgcttgggt cagcgcatcc gctcccagat gccggtttct tcgtccagcc tgtcctgttc
    10021 cagcccacgg aagtcgccgc gcgagacgat gccggcgact cggccgtcct gcaccaccgg
    10081 cacgtggcgg aagccgccat cgcgcatcag gcgcagggca tcgatcgcgg tggagcgcgg
    10141 cggcatgcat gcgggctcgc gcgtcatgac tccgtccagc gtggccgaaa cggggtcctt
    10201 gccctcggcc agcacccgga ccgcatcccg gccggtgaag atgccgatca gccggcaccc
    10261 cgggtcggtc accagaaccg cgccgatgcg gcgctgatgc atgtggcggc aggcttcctg
    10321 gacggtcgca ctgggtggca gcgtgagtgg tctctggtta cggatgatct ccgacatttc
    10381 ccggttcatg ttgccgctcc tggagcagtc atgcgcgaac aggttagcca cccgtaaacg
    10441 ccccggcctt gatccggatc aaaccgccgt tctgttgctg gcgagccgca aggcataatc
    10501 cactgcctcg agcaggctgt cctcgttggc cacgcccttg cccgcaatgt cgaaggcggt
    10561 gccgtgatcg accgaggtgc ggatcaccgg caggcccagc gtgacgttta ccccagacag
    10621 cgcctgccaa gcgccggtcg ccggatcgac ctggaatccc agcagcttga ccgggatgtg
    10681 tccctggtcg tggtacatcg ccaccacagc atcgaactgc cgggcgcgca gcttgacgaa
    10741 cacggtatcg cccggcaccg gcccggtcac cgccagcccc tcggccaggc actgcgccac
    10801 caccggtgtg gtcaccgtga tgtcctgggt gccgaacagc ccgccctcac cggcatgcgg
    10861 gttcagcgcg gcaaccgcaa tgcgcgggcg ggcaatgccc aggtcgagca gcgcggcgtg
    10921 ggtcaaatcg atggtgcggc gcagccgttc cggggtcagc agcgccggca ccttctccag
    10981 cgccacgtgg gtggtgacgt ggctgacccg catgtcgcca tgtgccagca gcatcaccga
    11041 gcttcgcgtg tgggtcagcg ccgccagcat gtcggtatgg ccggcgtagt ggtagccggc
    11101 gcggttcagc gcctccttgt tcagcggggc ggtgacgatg gcgtcgatcc gccccgcctg
    11161 cgccagtgcg acggcggttt ccactgccag gtaggcaaag cggccggctt ccggtgtcag
    11221 ttcgccgaag cggatcggcg ccagctcctc gccggccggc agcagcgcca gcggcggaaa
    11281 atccgcatcg cggccggttt ccgggaaact caggccagac tgcagcagat cgcaggaggc
    11341 acgcagcgca gagcggtggc cgatcaccag cagccgcatc tcgccggact gcagccgcgg
    11401 catcagccgg gcacaggcct tgacgatgat ctccgggccg accccggcgg gatcgcccat
    11461 ggtgatcgca agtcggggcg ggttggcggg catgctggct ctccgggcat tggcgttgca
    11521 tcggtaaaca cccgctgcac gcgcctgcca accgccagcc gccaaccgcc agccgccaac
    11581 cgccgctgat cggcggcagc cgccgccgcc ccgttcagtc cgccggttcg gccgccagac
    11641 gcggcgcctc ggtccgcacc tggtcgccca gcatctcgat gcggccggtg atctggccgc
    11701 catcctccac ctgcagccgc cggcagcgcg cggtgcccag cacccggccg gtggcccgca
    11761 ccaccaggct gccgcgcgcg gtcagcgtgc cgtcgatggt gccggcgatc tccgcatcct
    11821 cgacctcaac ctcgcccttg aacacgccgc cgggcgcgat cgccagttcg gtggcgtgga
    11881 tcatgctagc ctcgaccgtg ccttccacca caaggcgttc ggcgtcctgc acggtgccct
    11941 gcacgctgat gccgcggccc accaccaggg tgcgtcgctc gcccgggtcg cggttcgcgg
    12001 tgcgggaggg tgcggcggcc ggccgcgcgg gctggcccgg ggcagcggat gggctgccgg
    12061 gagtggcggg gaagggcgga cgagacatgg actgagcctc cttgggctgg gccggtcgga
    12121 acggcggcac gcccagcccg gaatcggccg ggccggaagc tgcgccaggg gtgcgcggat
    12181 gcaggtcggt tttccccgct tcctcggcga accggctggg ggaatcggtg gcggacagtc
    12241 cagccggcag agttccccct gatccggccg gcatttcgtc aggcttgcgg cgtttgaaca
    12301 ag
//
LOCUS       DASSZH010000250         5819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_977351, whole genome shotgun sequence.
ACCESSION   DASSZH010000250 DASSZH010000000
VERSION     DASSZH010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5819
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977351"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..912)
                     /gene="panC"
                     /locus_tag="VFN42_05345"
     CDS             complement(79..912)
                     /gene="panC"
                     /locus_tag="VFN42_05345"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HET8996074.1"
                     /translation="MQVVRTLAQLRSACASLRQLYGSVCLVPTMGALHAGHSALIRAA
                     VQSGGAVVASIFVNPLQFGPNEDLLRYPRDESGDLNRLEEAGCHLAWLPDVATMYPPG
                     DATTVNVNGPGENWEGAARPGHFRGVATVCAKLFGQVRPDRAYFGEKDWQQFQVVRRM
                     VADLLLPLEIVGIETVREPDGLAMSSRNRFLSAAERAVAPRLHATMQSVAKVLAAGGP
                     AAEVLAAAQRDLTHFGFAVDYFALVDGPTLHPLPSARSAARLIAAAKLGSVRLIDNIA
                     V"
     gene            952..1770
                     /locus_tag="VFN42_05350"
     CDS             952..1770
                     /locus_tag="VFN42_05350"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8996075.1"
                     /translation="MRSSPLVIAEHGRARMAGTGERLDMSLAGQIALVTGGSRGIGRA
                     TALAFAAEGADIAFCHLDDAAQAERTAAEIAALGRQAMHRSVDVADTAAVRAFAAAVA
                     AGFGPVDILFNNAGMNVRKPFEAYTEAEFDRILAVHLKGMFFMAQAVYPAMAARGRGC
                     IINVASQRALKGAVNSSVYSAAKGAIIGFTRALAWEAAPRGVRVNAIAPGPVDTDLTA
                     TMDPADRQAFIDALPAHRFGRPEEIAATALLLAGPQGGFYVGACLSPNGGDVMY"
     gene            complement(1786..2175)
                     /locus_tag="VFN42_05355"
     CDS             complement(1786..2175)
                     /locus_tag="VFN42_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HET8996076.1"
                     /translation="MHSNEVTQRMENRSIWLGIRRGLAKRCPACGQGHLFGGFLKVAP
                     HCDVCGADNSIYPSDDFPPYLTILAAGHLLVPLIFIVERSFAPALWLQVAIWLPVTVA
                     LCLALLPFMKGATVGLCWATGLVRNES"
     gene            complement(2401..3408)
                     /locus_tag="VFN42_05360"
     CDS             complement(2401..3408)
                     /locus_tag="VFN42_05360"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET8996077.1"
                     /translation="MPRFAAIGLDHRHIYDLTQGLLDAGAECAGYNPDTSDPRVLAGF
                     RKRFPHVPEAETARLLDDPGIDFVVIAAVPRDRAALAIAAMERGKDVMLDKPGVTTAA
                     QLAAVQRSVAETGRIWSIALGELTSPATQAALRVVRSGEIGRLLHTTALAPHRLNRAL
                     RPAWFFDTPAYGGIINDIGSHSIAQFLAFAGAADATIAHSTVCALGTEPPGFEDFAEL
                     ILSTPQVRGYIRLDWFTPDGLPDWGDGRLFVVGTEGTVELRKNLDIEGRPGSNHMFVA
                     NRSGTRYVDCSTAPVTYYRDFLADIANRSATTMPQAQVFTVCRLALEAQSQAARYTGS
                     R"
     gene            3543..4289
                     /locus_tag="VFN42_05365"
     CDS             3543..4289
                     /locus_tag="VFN42_05365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8996078.1"
                     /translation="MADRIAVITGAGTGIGRASAVALMNDGWSVALAGRRKEMLEETA
                     GMKKAGRALVVPTDVTDPQQVAHLFAQTKSEFGRVDMLFNNAGGGTPTTNFGDFTYEM
                     WLNVVQVNLNAMFLCAHAAFRMMREQTPRGGRIINNGSISAHVPRPGSVAYTATKHAV
                     TGLTKSIALDGREYDICSGQIDIGNAATPLTARSVNGQLQAHGAVVPEPRMDVNHVGQ
                     QVLFMANLPLESNVQFVTIMATKMPFIGRG"
     gene            complement(4499..5035)
                     /locus_tag="VFN42_05370"
     CDS             complement(4499..5035)
                     /locus_tag="VFN42_05370"
                     /inference="COORDINATES: protein motif:HMM:NF022467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2852 domain-containing protein"
                     /protein_id="HET8996079.1"
                     /translation="MSATNNEAYGNAAWGSGPAGGPWGPPPWAGQGWHDRPHWARDIP
                     RPVWIGFMILGFIFWWPVGLAMLVYLLASGRMSCGPRARHWANQQGGYQAGPGAGWGG
                     WARWCGGDRAPPSSGNRAFDDYRAETLRRLEEEQREFGAFLDRLRVAKDKAEFDQFMA
                     ERRGRPPQPQDGPQPPVQ"
     gene            complement(5124..5789)
                     /locus_tag="VFN42_05375"
     CDS             complement(5124..5789)
                     /locus_tag="VFN42_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET8996080.1"
                     /translation="MSWSESGGLGGGRRGYHHGNLREALVEATLELIAAKGPAGFTIA
                     EAARLAGVSPAAPYRHFRDAEALLAEVALRGFDQLTAVLTRAWANGTPDPVRAFEAIG
                     SAYLGFAREQPAYYAAMFEARIARDNHPGLLTAGDRAFAVLRDAAARLAESLPPDRRP
                     PAMMMALHIWALTHGIASLFVRPDVARRRLPMAPEDLLEAGVLLYLQSLGLAGGKPHR
                     TPG"
BASE COUNT          934 a         1991 c         2040 g          854 t
ORIGIN      
        1 acccacgtct ttgctggacg cagcacgaag ccatggatgg ccgcgccaag cgcggccacg
       61 acacatcggt ttgcctgact acaccgcgat attgtcgatc agccggaccg agccgagctt
      121 cgcggcggcg atgaggcggg cggcggatcg ggcggagggc agcgggtgca gggtcggccc
      181 gtccaccagg gcgaaatagt ccaccgcgaa gccgaaatgg gtcaggtcgc gctgagccgc
      241 ggccagcact tccgcggcgg gaccgccggc cgccagcacc ttggcgaccg actgcatcgt
      301 cgcatgaagc cgcggcgcca cggcgcgctc cgccgcgctc aggaagcggt tgcgggatga
      361 catcgccagc ccgtcgggct cccgcaccgt ctcgatgccg acgatctcca gcggcagcag
      421 caggtcggcc accatccggc gcacgacctg gaactgctgc cagtccttct cgccgaaata
      481 cgcccggtcc ggccggacct ggccgaacag cttggcgcag accgtcgcca cgccgcggaa
      541 atgtcccggc cgcgccgcgc cctcccagtt ctcgcccgga ccgttcacgt tgacggtcgt
      601 cgcgtcgccg ggcgggtaca tcgttgcgac gtccggcagc caggcgaggt ggcagccggc
      661 ttcctccagc cggttcaggt cgccggattc gtcgcgcgga tagcggagca gatcctcgtt
      721 gggaccgaac tgcagtgggt tgacgaaaat cgaggcaacc acggcgccgc cggattgcac
      781 cgccgcgcgg atcagcgcgc tgtggccggc atgcagcgcg cccatggtgg ggacgaggca
      841 caccgagccg taaagctggc gcagcgacgc gcaggcggaa cgaagttggg cgagggtacg
      901 aacgacttgc atgggcggaa cttggccctg gacggcggca ggggcaacat cgtgcgaagc
      961 agcccgctgg tgatcgccga gcatggcagg gctcgcatgg ctggaaccgg ggagcgtctc
     1021 gacatgagcc tggcaggaca gatcgcgttg gtgaccggcg ggtcacgcgg catcgggcgg
     1081 gccaccgccc tggccttcgc ggcggagggc gccgacatcg ccttctgcca tttggatgac
     1141 gcggcgcagg cggagcgcac cgcggcggag atcgcggcgc tgggccggca ggcgatgcac
     1201 cgttcggtgg acgtcgcgga taccgccgct gtgcgcgcct tcgccgccgc ggtggcggca
     1261 ggctttggac cggtcgacat cctgttcaac aatgccggga tgaacgtccg caagccgttc
     1321 gaggcctata ccgaggcgga attcgaccgc atcctcgccg tgcacctgaa gggcatgttc
     1381 ttcatggcgc aggcggtcta tccggcgatg gcggcacgcg gccgcggctg catcatcaat
     1441 gtcgcatcgc agcgggcgct gaagggcgcg gtgaattctt cggtgtactc ggcagccaag
     1501 ggcgcgatca tcggcttcac ccgggcgctc gcctgggaag cggcgccgcg cggcgtgcgg
     1561 gtgaacgcga tcgcccccgg tccggtcgac accgacctga ccgcgaccat ggacccggcg
     1621 gatcgccagg ctttcatcga tgccctgccg gcgcaccgct tcggccgccc ggaggagatc
     1681 gccgccacag cgctgttgct ggccggaccg cagggcgggt tctacgtcgg cgcctgcctg
     1741 tcgccgaacg gcggcgacgt gatgtactga cgccgccgcc tggcgtcagg actcgttgcg
     1801 gaccaggccg gtcgcccagc acaggccaac ggtggcgccc ttcatgaagg gcagcagtgc
     1861 gagacacaga gccaccgtca ccggcagcca gatcgcgacc tgcagccaca acgccggggc
     1921 gaagctgcgc tccacgatga agatcagcgg caccagcagg tggccggcgg ccaggatggt
     1981 cagatagggc gggaagtcgt cggacggata gatgctgttg tccgcaccgc agacgtcgca
     2041 atgcggcgcg accttcagga atccgccgaa caggtgcccc tgcccgcagg ccggacagcg
     2101 cttggccagg ccacgccgga tgccgagcca gatcgaccgg ttttccatcc gctgtgtgac
     2161 ctcattgctg tgcatcaacc ggcacccctc acccaatcgc agccaatata gggatgtgag
     2221 gatggcggca cacccggaag ggctgcatgt ctggctcccc cgccgcagag cctgtcgtgg
     2281 ttgccttact ggtggcgtgg cgtgcggatc cgcccgagcg tggcagggct ggccgcaccg
     2341 gcgcacctgg cggcgatcgt ctgaccgggg gcgtgagccc gccggtttcg cgtgccgctc
     2401 tcagcgcgaa ccggtgtagc gcgcggcctg cgactgcgcc tccaaggcga ggcggcagac
     2461 cgtgaacacc tgcgcctgcg gcatggtggt tgcgctgcgg ttggcgatat ccgccaggaa
     2521 gtcgcggtag taggtcaccg gtgcggtact gcaatcgacg tagcgcgtgc ccgagcggtt
     2581 ggcgacgaac atgtggttgc tgccaggccg gccttcgata tccaggttct tgcgcaattc
     2641 caccgtgcct tcggtgccga ccacgaacag cctgccgtcg ccccagtccg gcaggccgtc
     2701 cggcgtgaac cagtccagcc ggatgtagcc ccgcacctgc ggggtggaca ggatcagttc
     2761 ggcgaaatcc tcgaacccgg gcggttccgt tcccagggcg cagaccgtgc tgtgcgcgat
     2821 ggtggcatcc gccgcgccgg cgaaggccag gaactgcgcg atggaatgcg agccgatgtc
     2881 gttgatgatc ccgccatagg ccggcgtgtc aaagaaccag gccggccgca gcgcccggtt
     2941 cagccggtgc ggcgccagcg cggtggtgtg cagcagccgg ccgatttcac ccgaccgcac
     3001 cacgcgcagc gccgcctgcg tcgccgggga ggtcagctcg ccgagcgcga tcgaccagat
     3061 gcggccggtt tcggcgacgc tgcgctggac ggcggcgagt tgcgccgcgg tggtgacgcc
     3121 gggcttgtcc agcatcacgt ccttgccgcg ctccatcgcc gcgatcgcca gcgcggcgcg
     3181 gtcgcgcggc accgcggcga tcaccacgaa gtcgatgccg ggatcgtcca gcagccgcgc
     3241 cgtctcggct tccggtacgt gtgggaagcg cttgcggaag ccggcgagca cgcgtggatc
     3301 ggaggtatcc ggattgtacc cggcgcactc cgcgccggcg tccagcaggc cttgcgtcag
     3361 gtcgtagata tgccggtggt ccaggccgat cgcggcgaag cgtggcatgc aggatactcc
     3421 gagttccacg tcatggccgg gcatgacggc atgggacacc aatggctgga ggcttgcacc
     3481 atcttccccg ccccgcaacc aaggtccaca ctgtgcacag caaccaggag gggagacacg
     3541 gcatggcaga ccgtatcgcg gtcatcaccg gcgcgggcac cggcatcggc cgcgcttcgg
     3601 cggtggcgtt gatgaacgac ggctggtccg tcgcactggc cggccggcgc aaggaaatgc
     3661 tggaggagac cgccggcatg aagaaagccg gccgcgcgct ggtggtaccg acggacgtga
     3721 cggatccgca gcaggtcgcg catctgttcg cgcagacgaa gtcggaattc ggccgggtgg
     3781 acatgctgtt caacaatgcc ggcggcggca cgccgaccac caatttcggc gacttcacct
     3841 acgagatgtg gctgaacgtc gtgcaggtga acctgaatgc gatgttcctg tgtgcccacg
     3901 cggcattccg catgatgcgc gagcagactc cgcgcggcgg gcggatcatc aacaacggct
     3961 cgatctccgc gcatgtgccg cggccgggct ccgtcgcata caccgcgacc aagcacgcgg
     4021 tgaccggact gaccaagtcg atcgcgctgg atgggcgcga atacgatatc tgctccggcc
     4081 agatcgatat cggcaacgcc gccacgccat tgacggcgcg ctccgtcaac ggccagttgc
     4141 aggcgcatgg cgcggtggtg ccggagccac gcatggacgt gaaccatgtc gggcagcagg
     4201 tgctattcat ggcgaacctg ccgctggagt cgaacgtgca gttcgtcacc ataatggcga
     4261 cgaagatgcc gttcatcggc cgcggctagg cttcaacgtg tccgggtagg cgtcaacgcg
     4321 cttcaatgtg tcatggccgg gcttgacccg gccatctacc cgggatgttc gggcattcgg
     4381 gcgctgttgc gacccgaacc ttccggggcg ggtggccggg tccaagcccg gccacgacga
     4441 gagcgggatt agatgacacg ttccttcgcc ggccgtgtca cgtcggtgtc agccggcgct
     4501 attgcaccgg cggttgcggg ccgtcctgcg gctgcggcgg acggccgcgg cgctccgcca
     4561 tgaactggtc gaactccgcc ttgtccttgg ccacgcgcaa ccggtcgagg aaggcgccga
     4621 attcgcgctg ctcctcctcc aaccggcgca acgtctcggc gcgataatcg tcgaacgcgc
     4681 ggttgccgct gctgggcgga gcgcggtcgc caccgcacca gcgcgcccaa ccgccccagc
     4741 cggcgccggg ccccgcctgg tagccgccct gctggttggc ccagtgtcgc gcccgcggac
     4801 cgcacgacat gcggccggaa gccagcaggt acaccagcat tgccagcccc accggccacc
     4861 agaagatgaa accgaggatc atgaagccga tccagaccgg ccggggaata tcgcgcgccc
     4921 agtgcgggcg gtcatgccac ccctggccag cccagggcgg cggtccccaa gggccgcccg
     4981 caggcccact cccccaggcg gcattgccgt aagcctcgtt gttggtggcg ctcatcgcgg
     5041 ggtccccatg tgaatgtaag acacatttac atcatgggga cgccccaggg cggcgtcaag
     5101 caggatcgca ggctgccgct ggattatcct ggcgtgcggt ggggcttgcc gcccgccagg
     5161 ccgaggcttt gcaggtacag cagcacgccg gcctccagca ggtcctccgg cgccatcggc
     5221 agtcgccgcc gcgccacgtc cggccgcaca aacagcgatg cgatgccgtg cgtcagcgcc
     5281 cagatatgca gcgccatcat catggcgggc gggcggcggt ccggcggcag actctccgcc
     5341 agccgggccg cggcgtcacg cagcaccgcg aaggcgcggt cgccggcggt caacaggccg
     5401 gggtgattgt cgcgcgcgat gcgcgcctcg aacatcgccg cgtaatacgc tggctgctcg
     5461 cgtgcgaatc ccagataggc gctgccgatc gcctcaaagg cgcgcaccgg atcgggcgtg
     5521 ccattcgccc aggcgcgggt cagcaccgcg gtcaactggt cgaagccgcg caacgccact
     5581 tccgccagca aggcctcggc atcgcggaaa tgccggtacg gtgccgcggg gctgaccccg
     5641 gccagccgcg ccgcctccgc gatggtgaag ccggccggcc ccttggcggc gatcagctca
     5701 agcgtcgcct ccaccagcgc ctcgcgcagg ttgccgtggt gatagccccg ccgccctccg
     5761 ccgaggccgc ctgattcgga ccagctcatg tgaatggttc ttacatgcgc ggcggggca
//
LOCUS       DASSZH010000251         3677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_414675, whole genome shotgun sequence.
ACCESSION   DASSZH010000251 DASSZH010000000
VERSION     DASSZH010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414675"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VFN42_05380"
     CDS             complement(<1..253)
                     /locus_tag="VFN42_05380"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET8996081.1"
                     /translation="MSQEAVKGLVLLDIDARGVATVTLNRPEVGNAYNEDLLGALIEG
                     VQQLAPDPAVRCLVIRGAGKHFQAGADINWLRHAANYPPE"
     gene            complement(250..1179)
                     /locus_tag="VFN42_05385"
     CDS             complement(250..1179)
                     /locus_tag="VFN42_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HET8996082.1"
                     /translation="MLTRSDIAAAARRIAPHIRHTPVLRDSGSALGLDLPIVLKLELL
                     QHAGSFKPRGAFNRLLSGTIPSAGVVAASGGNHGAAVAYAARRLGVPAEIFVPTLTPA
                     AKVARIASYGATVSQIGETYAEALAASRVRQNESGALEVHAYDHPDVLAGQGTVGREF
                     ELDAPDVTHVLVATGGGGLIGGVAAWYAGSAAVVSVEPEGCPTLHTALREGRPVDAPV
                     GGLAADSLGARQVGGEMFPLAQRFVREAVLVPDEAIREAQRRLWDSFRLVAEPGGATA
                     LAALISGRFTPPADARVGVVVCGANTDPAKVTA"
     gene            1294..1728
                     /locus_tag="VFN42_05390"
     CDS             1294..1728
                     /locus_tag="VFN42_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphatase C-terminal
                     domain-containing protein"
                     /protein_id="HET8996083.1"
                     /translation="MNAPRQVYGSCAARDGHAVLVTGPSGAGKSDLVLRLLDRGFQLV
                     ADDRVELAGGYASAPAALAGLLEVRGLGILRLPYLQRARLALLVRIARETHRLPAPQQ
                     DAEFGVPVIRLIPDSASAPQRVALALDCALGRIGQVAGAFAA"
     gene            1725..2738
                     /gene="rapZ"
                     /locus_tag="VFN42_05395"
     CDS             1725..2738
                     /gene="rapZ"
                     /locus_tag="VFN42_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019090722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET8996084.1"
                     /translation="MTSNPPRRMRIVLVTGLSGGGKASILRALEDIGYEAVDNPPLPM
                     LEEMVSRGEGRLAIGVDARTRGFDARLVLDALDRLRANPALRPELVYAWADETTLLRR
                     YSESRRRHPLAPDGRVADGISMEREVTAALREGADLVVDTSELPLAALRRLIEGHFGE
                     GSDDSAHRLVVSLMSFSYARGLPREADLVFDARFLRNPHYDPILQPRTGLDPEVGAYI
                     RADPDYQEFFDKVAALVSLLLPRFVQEGKKYATITFGCTGGRHRSVYLVEALAAHLAA
                     ACDAGGADGEAGPAWRLHVTHRELAREGHQAAYLTERPVPRRGASDTGEAARASAAQA
                     QEA"
     gene            2811..3233
                     /locus_tag="VFN42_05400"
     CDS             2811..3233
                     /locus_tag="VFN42_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET8996085.1"
                     /translation="MIGMVLVTHGRLAQELRAAMEHVVGTQRNVDTVCIGPEDDMDNM
                     RSEIEACIQRCDTGDGVVLLTDMFGGTPSNLAISMMERKGVEVIAGVNLPMLVKLAKV
                     RSKQPLADAVLCAEEAGRKYIAAASHVLHGCRGNEPCS"
     gene            3249..3566
                     /locus_tag="VFN42_05405"
     CDS             3249..3566
                     /locus_tag="VFN42_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HET8996086.1"
                     /translation="MAADEAASPPESGAPVLSRTVTIINKRGLHARAAAKFVMVAERF
                     GASVDVLRDGQSVSARSIMGLMMLGAGRGAMLELRAEGWDAKEALDALAALVEAGFDE
                     QPD"
BASE COUNT          564 a         1224 c         1315 g          574 t
ORIGIN      
        1 gctccggcgg atagttcgcc gcatggcgca gccagttgat gtcggcgcca gcctggaaat
       61 gcttgccggc gccgcggatc accaggcagc gcaccgccgg atcgggcgcc agctgctgca
      121 cgccctcgat cagcgcgccc agcaggtcct cgttataggc gttgccgacc tccggccggt
      181 tcagcgtcac cgtggcgacg ccacgggcat cgatatccaa caggacgagg cccttcacgg
      241 cttcctggct catgctgtca ccttggctgg atcggtattg gcaccgcata ccacaacgcc
      301 gacccgcgca tcagccggcg gcgtgaagcg gccggaaatc agcgccgcca gggcggtggc
      361 gccgccgggt tcggcgacca gacggaagct gtcccacagc cggcgctgcg cctcgcggat
      421 cgcctcgtcc ggcacgagca cagcctcacg gacgaagcgc tgggcgagcg ggaacatctc
      481 cccgccgacc tggcgggcgc ccaggctgtc cgccgccagc ccgccgaccg gggcatccac
      541 cgggcggccc tcgcgcagcg cggtatgcag ggtggggcag ccttcaggct ccacgctcac
      601 caccgcggcg ctgccggcgt accaggcggc caccccgccg atcagcccgc cgccgccggt
      661 ggcgaccagc acatgcgtca cgtcaggggc atccagttcg aactcgcgcc cgacggtgcc
      721 ctggccggcc agcacgtccg gatgatcata ggcgtgcacc tccagggcgc ccgactcgtt
      781 ctgccggact cgggacgcgg ccagcgcctc ggcataggtc tcaccgattt gtgagaccgt
      841 cgcgccgtag ctggcgatgc gcgccacctt ggccgccggc gtgagtgtcg ggacgaagat
      901 ttccgcggga accccaaggc ggcgcgcggc ataggcaact gccgcgccgt gattgccgcc
      961 ggatgcggcg accacgccag cggacggaat cgtaccggat agcagacgat tgaacgcgcc
     1021 acgcggcttg aagctgccgg catgttgcag cagttcgagc ttcagcacga tcggaaggtc
     1081 gaggccgagt gccgagccgg agtcgcggag cacaggcgtg tggcggatgt ggggagcgat
     1141 gcggcgagcg gcggcagcga tatcggagcg ggtcagcatg ggccggacgc tggcatgcgg
     1201 gcagccgcgg gacaaggccg cggcgcggaa caaaacgcgc tcgcgtgcgg gggtgctttc
     1261 attccctctc gcttcttgcc agattgctcc tccatgaacg ccccgaggca ggtttatggc
     1321 agttgtgccg ccagggacgg ccacgccgtg ctggtgaccg gtccgtccgg ggcaggcaaa
     1381 tccgatctgg tgcttcggct gctggatcgc ggattccagc tcgtggcgga tgaccgtgtc
     1441 gaactcgccg gcggctatgc cagtgcgccg gccgcgctgg ccggattgct ggaagtgcgc
     1501 ggcctgggga tcctgcggct gccgtacctc cagcgtgccc ggctcgctct gctggtgcgg
     1561 attgcccggg aaacccatcg cctgccggcg ccgcagcagg atgcggaatt cggcgtgccg
     1621 gtgatccggc tgatcccgga tagcgcctcg gccccgcagc gggtcgccct ggcactggat
     1681 tgcgcgctcg gccgcatcgg ccaggtcgcc ggagcgtttg cggcatgaca tccaacccgc
     1741 cccggcggat gcggatcgtg ctggtcactg gcctgtccgg cggtggcaag gcctcgatcc
     1801 tgcgtgcgct ggaggatatc ggatacgagg ccgtggataa cccgccgctg ccgatgctgg
     1861 aggaaatggt cagccgcggc gaaggccggc tggcgatcgg cgtggacgcc cgaacccggg
     1921 gcttcgatgc ccggctggtg ctggatgcgc tcgaccggct gcgcgccaac ccggcattgc
     1981 ggccggagct ggtctatgcc tgggccgacg aaaccacctt gctgcgccgc tactctgaaa
     2041 gccgccggcg gcatccgctg gcgccggacg gcagggtggc ggacggcata agcatggaac
     2101 gcgaggttac cgccgcgttg cgtgagggcg ccgacctggt ggtggatacc tccgagctgc
     2161 cgctggctgc cttgcggcgg ctgatcgagg ggcatttcgg ggaaggcagc gacgactcgg
     2221 cgcaccgtct cgtggtgtcg ctgatgtcgt tttcctacgc acgtggcctg ccgcgggaag
     2281 cagatctggt attcgatgcc cgctttttgc ggaatccgca ctacgacccc atattgcagc
     2341 cgcgcacggg gctggatcct gaggtcggcg cctatatccg ggccgatccg gattaccagg
     2401 aatttttcga caaagtggcg gcgctggtgt cgctgctctt gccgaggttc gtgcaagagg
     2461 gcaagaaata cgcgacaatc acgttcgggt gtacgggcgg gcgtcatcgc tccgtatatc
     2521 tggtcgaagc gttggccgct catctggccg ctgcgtgtga cgctggcggt gcggacggag
     2581 aggctggtcc tgcctggcgg ctgcatgtga ctcatcgcga attggcacgc gagggccacc
     2641 aggccgcgta tctgacggaa cgcccggtcc cgcgccgggg cgcaagcgat actggcgagg
     2701 cagcgcgggc ctcggcggct caggcacagg aggcctgata cacatatgtg cggccatcaa
     2761 gacagtgggc ttcgccgtgc cggagccggt agggaagtta ctcaccttcc atgatcggca
     2821 tggttctggt gactcacggc cggctcgccc aggagcttcg tgcggcgatg gagcacgtcg
     2881 tcggtacgca acggaacgta gacaccgtct gtatcgggcc cgaggacgac atggacaaca
     2941 tgcggtccga gatcgaggcc tgcatccagc gttgcgatac cggtgacggg gtggtcctgc
     3001 tcacggacat gttcggtggc acaccgtcca acctggccat ctccatgatg gagcggaaag
     3061 gggtggaggt gatcgcgggc gtcaacctgc cgatgctggt gaagctggcc aaggtgcgct
     3121 ccaagcagcc gctcgccgac gcggtgctgt gcgccgagga ggccgggcgc aaatacatcg
     3181 ctgccgcctc ccacgtgctg cacggctgcc gcggcaacga gccatgttcg tgaccgcatg
     3241 gccggcgcat ggcggctgac gaagcggcct cgccgccgga aagcggcgca cccgtcctga
     3301 gccggacggt caccatcatc aacaaacgag ggctgcatgc ccgcgccgcc gccaagttcg
     3361 tcatggtggc agagcggttc ggcgcgtcgg tggacgtgct gcgcgatggc cagagcgttt
     3421 ccgcccgctc catcatgggg ctcatgatgc tcggggccgg ccggggcgcc atgctggagc
     3481 tgcgcgccga aggctgggat gcaaaggaag cgcttgacgc gctggccgcc ctcgtcgagg
     3541 ccggcttcga tgaacagccg gactgacccg cccccgccac agcaccgccg cctgaaccgc
     3601 gccgccgcgc gcgagcaggt gccggagcgc gtgttcaccg gcatcccggt cgcgcccggc
     3661 atcgccatcg ggccggt
//
LOCUS       DASSZH010000252         3520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_483094, whole genome shotgun sequence.
ACCESSION   DASSZH010000252 DASSZH010000000
VERSION     DASSZH010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483094"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="VFN42_05410"
     CDS             complement(<1..995)
                     /locus_tag="VFN42_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014457165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8996087.1"
                     /translation="MSLLVITGATIRLGGRTLLDGADLTIEPGRRIGLVGRNGAGKST
                     LLRAIAGELPLDGGNMRFSSRARLATVKQEAPSGGASLLDTVLQGDPERLALLAESET
                     ADPMRLAEIHERLIAIGAESAPARAAAILAGLGFDEAAQARPVDEYSGGWRMRVALAT
                     ALFAAPDLLLLDEPTNHLDLEATLWLEGWLSRFPGAALIVSHDRGLLDRAVEAIAHLD
                     RGKISLTPGGFDEFVRIRTERAMQQAAMAAKVAAQRAHMQAFVDRFRYKASKARQAQS
                     RIKALERLPQIDSVIEDAPTKFDFPEPARIAPPILALERVSIGYDGVPVLRDVSLT"
     gene            1386..1901
                     /locus_tag="VFN42_05415"
     CDS             1386..1901
                     /locus_tag="VFN42_05415"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET8996088.1"
                     /translation="MSTNSELKARLARLGPVRDADPPPSFSGALVPVVLKLDGALDKP
                     IDVANRLRAAGLTLRAAHAIINRLAETRLAVCQIAEGADILALAADLARMNVHMHRRR
                     RLDPGLVTQVRARHGLSQREFAAVIGVDTDTLQNWEQQRNRPDDAALSLVLAFDAAPD
                     VIERALFEQVA"
     gene            complement(1957..2187)
                     /locus_tag="VFN42_05420"
     CDS             complement(1957..2187)
                     /locus_tag="VFN42_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996089.1"
                     /translation="MAFDTTVETYPRTEAVRRALAAAQNTGAAADLIFLERWVTAPSH
                     DLAATLRASQIRRANPTLADAIQLELSARPGR"
     gene            complement(2315..>3520)
                     /gene="ahcY"
                     /locus_tag="VFN42_05425"
     CDS             complement(2315..>3520)
                     /gene="ahcY"
                     /locus_tag="VFN42_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006116224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET8996090.1"
                     /translation="LMSLRKEYGEEKPLAGARISGSLHMTVQTAVLIETLVALGAEVR
                     WASCNIFSTQDHAAAAVAAAGTPVFAWKGMNEEEFWWCIDQTVRGPDGWTPNMILDDG
                     GDLTLLMHQKYPEMLKDVRGISEETTTGVHRLRDMARDGKLLTPAINVNDSVTKSKFD
                     NLYGCRESLVDGIRRGTDVMMAGKIAVVNGFGDVGKGSAASLRNAGCRVMVTEVDPIC
                     ALQAAMEGYQVVTMDDAAPIGDIFVTATGNVDVITIDHMRAMKHRAIVCNIGHFDSEI
                     QIEALRNYKWENVKPQVDEVVFPDGKRLIVLSEGRLVNLGNATGHPSFVMSASFSNQV
                     LAQIELFNAPAGKYQRQVYTLPKQLDEKVAAFHLAKVGAKLTKLSAQQAEYIGVTAEG
                     PFKHDLYRY"
BASE COUNT          539 a         1148 c         1200 g          633 t
ORIGIN      
        1 gtaagggaga catcgcgcag caccggcacg ccgtcatagc caatgctcac acgctccagc
       61 gccaggatcg gcggggcgat tcgcgctggc tccggaaagt cgaatttggt gggcgcgtcc
      121 tcgattaccg aatcgatctg cggcaggcgt tccagcgcct tgatgcggga ctgcgcctgg
      181 cgggccttgc tggccttgta gcggaagcgg tccacgaagg cctgcatgtg ggcgcgctgc
      241 gccgccacct tcgccgccat ggcggcctgc tgcatggcgc gctcggtgcg gatgcggacg
      301 aactcgtcga agccgccggg ggtcaggctg atcttgccgc ggtcgagatg ggcgatcgcc
      361 tccaccgcgc ggtccagcag gccgcggtcg tggctgacga tcaaggcggc gccggggaag
      421 cgggacagcc agccctccag ccacagcgtg gcttccaggt ccaggtggtt ggtgggctcg
      481 tccagcagca gcaggtcggg ggcggcgaac agcgcggtgg ccagggcgac gcgcatgcgc
      541 cagccgccgg agtattcgtc caccgggcgc gcctgggcgg cctcgtcgaa gcccagaccg
      601 gcgaggatag cggcggcgcg ggcgggcgcg gattcggcgc cgatggcgat cagccgctcg
      661 tggatctccg ccagccgcat cgggtcggcg gtttccgact ccgccagcag ggcgaggcgt
      721 tccgggtcac cctgcagcac cgtgtccagc aggctggcgc cgccggacgg cgcttcctgt
      781 ttcaccgtgg ccagccgggc gcgggaggag aaccgcatgt tgccgccgtc cagcggcagc
      841 tccccggcga tggcgcgcag cagggtggat ttcccggcgc cgttgcggcc gaccaggccg
      901 atgcggcggc ccggctcgat cgtcaggtcg gcgccgtcca gcagcgtgcg cccgccaagg
      961 cggatggtgg cgccggtgat gaccaggagg gacatggcgg gcttgatcgg acgggcggcg
     1021 gcccgtggtc aaggggatgc gggcggctcg gcaaatcgcc cgttctgacc tagccttctt
     1081 ggcgttccat ttgacgagca tacgcatttc gcgtatagga aattcaggcg cgtcgccgat
     1141 cctttattcg tttcgcagtc gagggacggt gatggcgtgg cagctgctca gccttgaact
     1201 cgactctggc gacgtctgtc ttgcccggtt ctcaaatggg agcgacgagc tgtcggcggt
     1261 gttctatgcc gaacttcaca atcgaactgc cgtgctgcga cgaactgaca ttcaaggtgc
     1321 gggaccaaac agcctgggat ggccggcact gcgtcaattc gcgcaccggc tgatggaggc
     1381 gctgagtgtc gacgaactcc gaattgaagg cgcggctcgc acgtctgggg cctgtccggg
     1441 acgccgaccc gcccccatca ttttccggcg ctctggtgcc ggtggtgctt aagctggacg
     1501 gagcactcga caaacccatc gacgtggcca atcgcctgcg ggcagccggc ctcaccctgc
     1561 gcgcggcgca tgccatcatc aaccggctgg cggagacgcg gctggccgtc tgccagattg
     1621 ccgaaggcgc cgatattctc gcgctcgccg ccgatctggc tcgcatgaat gtccacatgc
     1681 atcgccggcg ccggctcgat ccgggtctgg tcacccaggt gcgggcgcgg cacggtctat
     1741 cgcagcggga atttgccgcg gtgataggcg ttgataccga cacgctgcag aactgggaac
     1801 aacaacgcaa tcgccccgat gacgcggcgc tcagcctggt tctggccttc gacgccgcgc
     1861 cggacgtgat cgagcgcgcc ttgttcgagc aggtcgcctg acgcatgcag gcgtagggaa
     1921 tccctctccg ccccgcaggt cggagaggga tcccagtcag cgtccgggcc gcgcggacag
     1981 ctccagttgg atggcatcag ccagggtggg gttggctctg cggatctggc tcgcgcggag
     2041 cgtcgcggcg agatcgtggc tgggggccgt cacccagcgt tccaggaaga tcaggtcggc
     2101 cgcggcgccg gtgttctgcg ccgcagccag tgcccggcgc accgcctcgg tgcgcgggta
     2161 ggtttccaca gttgtgtcga aagccatggt gtcgtcctgt cggtgatgcc atcacgccgg
     2221 agcatgatgg cagcgccgag ttaaggacgt ctcaacaact ccgccactgc gagacgatga
     2281 cagtgtggtg aaggattttt tcggccagct ctgctcagta gcggtacaga tcgtgcttga
     2341 acggtccttc cgccgtcacg ccgatatatt cggcctgctg cgctgacagt ttcgtgagct
     2401 tggcgcccac ttttgccagg tggaatgcgg ccactttctc atcgagctgc ttcggcaggg
     2461 tgtagacttg gcgctggtac ttgccggcgg gcgcattgaa caattcgatc tgcgccagaa
     2521 cctggttgct gaagctggcg ctcatcacga agctgggatg ccccgtcgcg ttgcccaggt
     2581 tcaccaaccg gccttccgac agcacgatca gccgcttgcc gtcggggaac accacctcgt
     2641 ccacctgcgg cttcacgttc tcccacttgt aattgcgcag cgcctcgatc tggatctcgg
     2701 aatcgaagtg gccaatgttg cacacgatgg cgcggtgctt catcgcccgc atgtggtcga
     2761 tggtgatcac gtccacattg ccggtcgcgg tgacgaagat gtcaccgatc ggtgcggcat
     2821 cgtccatcgt caccacctgg tagccttcca tcgccgcctg cagcgcgcag atcggatcga
     2881 cctcggtcac catcacgcgg cagccggcat tgcgcaggct ggccgcgctg cccttgccga
     2941 catcgccgaa gccgttcacc accgcgatct tgccggccat catcacgtcg gtgccacggc
     3001 ggatgccgtc caccagcgat tcgcggcaac catacaggtt gtcgaacttg ctcttggtga
     3061 cgctgtcgtt gacgttgatc gccggcgtca gcagcttgcc gtcgcgcgcc atgtcgcgca
     3121 gccggtgcac gccggtggtg gtctcctcgg aaatgccgcg cacgtccttc agcatctcgg
     3181 gatatttctg gtgcatcagc agcgtcaggt cgccgccgtc atccaggatc atgttcgggg
     3241 tccagccgtc cgggccgcgc accgtctggt cgatgcacca ccagaactct tcctcgttca
     3301 tgcccttcca ggcgaacacc ggggtgccgg cggccgccac ggcggcggcg gcatggtcct
     3361 gggtggagaa gatgttgcac gacgcccagc ggacctcggc cccgagggcc accagtgtct
     3421 cgatgagcac ggcggtctgc acggtcatgt gcagcgagcc gctgatgcgg gcaccggcga
     3481 ggggcttctc ctcgccgtac tccttgcgca gcgacatgag
//
LOCUS       DASSZH010000253         2042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_329694, whole genome shotgun sequence.
ACCESSION   DASSZH010000253 DASSZH010000000
VERSION     DASSZH010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329694"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VFN42_05430"
     CDS             <1..209
                     /locus_tag="VFN42_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8996091.1"
                     /translation="IDQQVWVLEGTLEVRAGDAAHRLATGDCLAMRVDRPTGFHNPGH
                     EATRYLVALSTAAAKSPGKAGSEA"
     gene            206..739
                     /locus_tag="VFN42_05435"
     CDS             206..739
                     /locus_tag="VFN42_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8996092.1"
                     /translation="MTETAVTVRTLTAEQAYASRAALADVLIDCVEGGASVSFMPPLT
                     RERAERFWTGVADGVATGERRLLVAQDQQGALLGTVQVVLAQPENQPHRGDVAKMLVR
                     RSARRCGIGGRLMQAAEVSAREGGKTLLVLDTATGSDAERLYQRLGWVRVGIVPGYAL
                     WPDGSPCATTYYFKTLV"
     gene            complement(810..914)
                     /gene="cydX"
                     /locus_tag="VFN42_05440"
     CDS             complement(810..914)
                     /gene="cydX"
                     /locus_tag="VFN42_05440"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HET8996093.1"
                     /translation="MWYFSWILGLSFAAAFASLNAMWLELAPGKGLPD"
     gene            complement(927..>2042)
                     /gene="cydB"
                     /locus_tag="VFN42_05445"
     CDS             complement(927..>2042)
                     /gene="cydB"
                     /locus_tag="VFN42_05445"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HET8996094.1"
                     /translation="LIWWAVLGVLLIGIAVMDGFDMGTGILLPWVGRNDMQRRIVINT
                     VGPVWEGNQVWLILGGGAIFAAWPAIYAVAFSGFYLAMLLLLCSLILRPVGFKFRSKI
                     SDPRWRETWDWALFIGGLVPALVFGVAFGNVLQGVPFRFDETLRMTYTGSVFGLFNPF
                     ALLCGLVSVAMLTMHGGAWLAVKTEGALSRRAQGAALVASLATLVLFALGGFWAARLN
                     GYLLQSFPGMAEPSNPLNKQVVRAAGVLMHNYGVAPWTMLAPAIGLGGAALAALLLLA
                     RRLPKLAFLCSAFSIAGIIATAGVSLFPFMLPSSIDPVSSLTVWDASSSLTTLIIMTG
                     ATAIFLPIILLYTAWVYHVLRGPVTPETIEADPHISY"
BASE COUNT          385 a          707 c          690 g          260 t
ORIGIN      
        1 cgatcgacca gcaggtatgg gtgcttgaag gcacgttgga ggtgcgcgcc ggagacgccg
       61 cgcacaggct tgccaccggc gactgcctcg cgatgcgggt ggatcgtccc accggcttcc
      121 acaatcccgg ccacgaggcc acgcgctatc tggtcgcgct gagcacggcg gccgccaaat
      181 ccccagggaa agcagggagt gaagcatgac cgaaactgcg gtcacggtgc ggaccctcac
      241 cgcggaacag gcttacgcga gccgagcggc gctcgcggac gttctgatcg actgcgtgga
      301 aggcggagcc tcggtcagct tcatgccgcc gctgacacgc gagagggccg agcggttctg
      361 gaccggcgtt gccgacggtg tcgccacggg tgaacgccgc ctgttggtgg cgcaggatca
      421 acagggcgcg ttgctgggca cggtgcaggt ggtgctcgcg caaccggaga accagccgca
      481 ccggggcgat gtcgccaaga tgctggtgcg ccgctcggcc cgccgctgcg gcatcggcgg
      541 ccggctgatg caggctgcgg aagtcagcgc acgcgagggc ggcaagacgc tgctggtact
      601 cgacaccgcc accggcagcg atgcggaacg gctgtaccag cggctgggct gggtccgcgt
      661 gggaatcgtc cccggctacg cactgtggcc ggatggaagc ccgtgcgcga ccacgtacta
      721 tttcaagacg ctggtctgac gccccggcag gttcccggcc ggcagcccgc aaaccgggtg
      781 ccggccgagc ccgccgcctc gtcgcggtct cagtcgggca ggcccttgcc gggcgccagc
      841 tccagccaca tggcgttcag cgaggcaaag gccgcggcaa agctcaggcc caggatccag
      901 gagaagtacc acatcgagac aacacctcag tagctgatat gcggatcggc ttcgatcgtt
      961 tccggcgtca ccgggccacg cagcacgtga tagacccagg cggtgtagag caggatgatc
     1021 ggcaggaaga tcgccgtcgc gccggtcatg atgatcagcg tcgtcaacga cgacgaagca
     1081 tcccacaccg tcaggctgga caccggatcg atcgaggatg gcagcatgaa ggggaacagg
     1141 ctgacgccgg ccgtggcgat gattccggcg atgctgaagg cgctgcacag gaaagccagc
     1201 ttcggcagcc ggcgcgccag cagcagcagt gccgccaggg ccgcgccgcc caggccgatc
     1261 gccggcgcca gcatggtcca gggcgccacg ccgtagttgt gcatcagcac gccggctgcg
     1321 cgcaccacct gcttgttcag cggattggac ggctccgcca tgccggggaa agactgcaac
     1381 agatagccgt tcaggcgcgc cgcccagaag ccgcccagcg cgaacagcac cagcgtcgcc
     1441 agtgaggcaa ccagcgcggc gccctgcgcc cgccggctca gcgcgccttc cgttttcacc
     1501 gccagccagg caccgccgtg catggtcagc atcgccacgc tgaccagccc gcacagcagc
     1561 gcgaaggggt tgaacagccc gaacaccgag ccggtgtagg tcatgcgcag cgtctcgtcg
     1621 aaacggaacg gcacgccctg cagcacattg ccgaacgcca cgccgaacac cagcgccggc
     1681 accaggccgc cgatgaacag cgcccagtcc caggtctccc gccagcgcgg atcgctgatc
     1741 ttgctgcgga acttgaagcc gaccggccgc aggatcagcg agcacagcag cagcagcatc
     1801 gccagataga agccggagaa cgccaccgcg tagatcgccg gccaggcggc gaagatcgcc
     1861 ccgccgccca ggatcagcca cacctggttg ccttcccaca ccgggccgac ggtattgatg
     1921 acgatgcggc gctgcatgtc gttgcggccg acccagggca gcaggatgcc ggtgcccatg
     1981 tcgaacccgt ccataaccgc gatgccgatc agcagcaccc ccagcacggc ccaccagatc
     2041 ag
//
LOCUS       DASSZH010000254         2896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_278654, whole genome shotgun sequence.
ACCESSION   DASSZH010000254 DASSZH010000000
VERSION     DASSZH010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278654"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..1189)
                     /locus_tag="VFN42_05450"
     CDS             complement(122..1189)
                     /locus_tag="VFN42_05450"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET8996095.1"
                     /translation="MTDIPAAPDMPPQIGRYRVQRLLGQGAMGVVYAAHDPVIDRTVA
                     IKLMRIDLLEGTDRADFVARFQREAQAAARCMHPNIVAVYDFALHEGNPFLAMEFVQG
                     TSLATRLRQGPRFAPEEAVAILRQMLDALGGAHAAGVVHRDVKPANILLLDGNRVKMT
                     DFGIARFDGSELTQTGAVIGTPSYMSPEQCRGDPVDARSDLFSAGAVFYEMLTGERPF
                     GGGNATQVIYRLMQEEPPALVTPHPLMPANLAAVLRRALAKRAADRFASAEAMAAALQ
                     APADATVIAPPVSTSPARIAPDEVARAEQALAEYLGPIARVLVKRALPDARSPAALWA
                     ALAAHIDGAADRAAFLRQAAG"
     gene            complement(1182..2054)
                     /locus_tag="VFN42_05455"
     CDS             complement(1182..2054)
                     /locus_tag="VFN42_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HET8996096.1"
                     /translation="MGLKAKFNLVMVAAFVIGLGLVAAWSWQVVHQAARHEVQQQAEL
                     MIAQGSAIRHYTDTQIDPLLAEQNKARFLPQSIPFYAAQETLRTLSRQFPAYSYKEAA
                     LNPTNPADRAVDWEAGIIDMFRRQPTLQTCTLTHETAAGPVLSVAHPVRINDQSCLAC
                     HSTPQAAPPSMIDLYGSKNGFGWKLGEVIGAQIVSVPMRVALERANATFAAYVGGLAA
                     ILAVLLLVLNLLLHAIVVKPVRNIAARAGEISTGNLDVPEFAVQGRDEIASLATSFNR
                     MRRSLARALKMLGD"
     gene            complement(2067..>2896)
                     /locus_tag="VFN42_05460"
     CDS             complement(2067..>2896)
                     /locus_tag="VFN42_05460"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HET8996097.1"
                     /translation="AMFAGIATLARRIKAAPDAPVFVYVCGYASAFDARPFLLPVSAQ
                     VARPADLLTQGILAQSVVDAVTRNTKAPALIVLDAIPAPAAPDSLALERLAQDLPDSV
                     GLLAVRDSGTGDQPTPLAAILTPALHGPALKGAALLAELKPQLNGHPTPQIAALHMPS
                     DQLYLAGAVTPAAPAAATPAIPAAPAATAAPSAAPAAAPTAAPRPAAALPDEDQMTEA
                     QRQQVQQALATLGYYDGRIDGIFGPETRAAIRRWQHEAHAPMTGHLTASQATQLISG"
BASE COUNT          434 a          991 c         1017 g          454 t
ORIGIN      
        1 gcgttcggca ttcaccgcgg gacgagggcg agatctcggg cggcggattg tggcgcaacc
       61 tgctgttcgg cggaccggac ctgtcgcgcc gacgaagcct ctcaggcgcg tgacgagagg
      121 cttatccggc cgcctgccgc aggaaggccg cgcggtctgc cgcgccgtcg atatgcgccg
      181 ccagcgctgc ccacagcgcg gcgggcgagc gtgcatccgg cagcgcccgc ttcaccagca
      241 cgcgggcgat cggcccgaga tactcggcga gtgcctgctc cgcccgtgcc acctcgtccg
      301 gtgcgatccg cgcagggctg gtggaaaccg gcggcgcaat caccgttgcg tcggccggtg
      361 cctgcaacgc ggcggccatc gcctcggccg acgcgaaccg gtccgccgcg cgcttggcca
      421 gcgcccgccg cagcaccgcg gccaggttcg ccggcatcag cgggtgcggg gtgacaagcg
      481 ccggaggctc ctcctgcatc agccggtaga tcacctgcgt ggcgttgccg ccgccgaagg
      541 ggcgttcgcc ggtcagcatc tcgtagaaaa ccgcaccggc cgagaacagg tcgctgcgtg
      601 cgtccaccgg atcgccgcgg cactgctcgg gtgacatgta gctgggcgtg ccgatcaccg
      661 cgccggtctg ggtcaactcg gaaccgtcga agcgggcgat gccgaaatcg gtcatcttca
      721 cccggttgcc gtccaacagc aggatgttgg cgggcttcac gtcgcgatgc accacgccgg
      781 cggcatgcgc gccgcccagc gcgtccagca tctgccgcag gatcgccacc gcttcctccg
      841 gtgcgaaacg cggcccctgg cgcaaccgcg tggcgaggct ggtgccctgc acgaattcca
      901 tcgccaggaa cggattgcct tcgtgcagcg cgaaatcata caccgcgacg atgttcggat
      961 gcatgcagcg ggcggcggcc tgtgcctccc gctggaagcg cgccacgaaa tccgcccggt
     1021 ctgtgccttc cagcaggtcg atgcgcatca gcttgatcgc caccgtgcgg tcgatcaccg
     1081 gatcatgcgc cgcgtacacc acgcccatgg cgccctggcc cagcaggcgc tggacgcggt
     1141 agcggccgat ctgcggcggc atgtccggtg cggcgggaat gtcagtcacc cagcatcttc
     1201 aaggcccgcg ccaggctgcg ccgcatgcgg ttgaaggacg tggcgagcga ggcgatttcg
     1261 tcgcggccct gcactgcgaa ttccggtacg tccagattgc cggtgctgat ctcgccggcg
     1321 cgagcggcga tattgcgcac cggcttcacc acgatggcgt gcagcagcag gttcagcacc
     1381 agcagcagca ccgccaggat cgccgccagc ccgccaacat aggcggcgaa ggtcgcattg
     1441 gcgcgctcca atgccacccg catcggcacg gagacgatct gcgccccgat cacctcaccc
     1501 agcttccaac cgaacccgtt cttgctgccg tacaggtcga tcatcgaggg cggggccgct
     1561 tgcggcgtgg agtggcaggc caggcagctc tgatcgttga tccgcaccgg atgcgcgacc
     1621 gacaacaccg gtccggcggc tgtctcatgc gtcagcgtgc aggtctgcag ggttggctgc
     1681 cgacggaaca tgtcgatgat gccggcttcc cagtccaccg cacgatctgc cggattggtc
     1741 ggattgagcg cggcctcctt gtagctgtaa gccgggaact gccgggacag cgtgcgcagc
     1801 gtttcctgcg cggcgtagaa cggaatgctc tgtggcagga agcgcgcctt gttctgctcc
     1861 gccagcagag gatcgatctg cgtatcggtg tagtgccgga ttgccgaacc ctgggcgatc
     1921 atcaattcgg cctgctgctg cacctcgtgc cgagcggcct ggtggacgac ctgccaggac
     1981 caggcggcga ccaggcccag gccgatcacg aaggctgcca ccatcaccag gttgaacttg
     2041 gccttcaggc ccatggccgg acgctttcag ccgctgatga gttgcgtggc ctgcgaagcg
     2101 gtcaggtgac ctgtcatcgg cgcatgcgct tcgtgctgcc agcggcgtat ggcggcacga
     2161 gtctccggcc cgaagatgcc gtcgatgcgg ccatcatagt agcccagcgt tgccagcgcc
     2221 tgttgtactt gctgccgctg cgcctccgtc atctggtctt cgtccggcag cgccgccgcc
     2281 ggccttggcg cagccgttgg cgccgcggcc ggcgcagcgc ttggcgcggc tgtcgctgcg
     2341 ggtgccgccg gaatggccgg cgttgctgcg gcgggcgctg cgggggtgac agcccccgcg
     2401 aggtacaact ggtcggaggg catgtgcagg gcggcgatct gcggcgtcgg atgaccgttg
     2461 agttgtggtt tcagctcggc cagcagcgcc gcgcccttca gtgcgggacc gtgcagggcg
     2521 ggagtcagga tggcggccag cggcgtcggc tggtcgcccg ttccggagtc gcgcaccgca
     2581 agcagtccga ccgagtcggg caggtcctgc gccagccgct ccagcgccag gctgtcgggc
     2641 gctgctgggg ccgggatcgc gtccagcacg atcagcgccg gcgccttggt gttacgggta
     2701 acggcatcca ccacgctttg ggccaggatg ccctgggtca gcagatcggc cgggcgggcg
     2761 acctgggccg agacgggcag caggaacggc cgcgcatcga aggcgctggc gtagccgcag
     2821 acatagacaa aaaccggggc gtccggagcc gccttgatcc gccgggccag cgtggcgata
     2881 ccggcaaaca tcgcgc
//
LOCUS       DASSZH010000255         2015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1079568, whole genome shotgun sequence.
ACCESSION   DASSZH010000255 DASSZH010000000
VERSION     DASSZH010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079568"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            430..1017
                     /locus_tag="VFN42_05465"
     CDS             430..1017
                     /locus_tag="VFN42_05465"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HET8996098.1"
                     /translation="MTIDAAGLDLLFREARSHNKFTDEPISDETLRELYAIVKMGPTS
                     ANSSPARFLFLRSKEAKERLAPALSSGNLEKTMAAPVTVIVAYDPKFHEKLPQLFPHN
                     PDAQSWFTSNDSLAATTAFRNGTLQGAYLILAARALGLDTGPMSGFDNAKVDEEFLSW
                     RGWRSNFLVNIGHGDPSGVFPRSPRLEFDEACDLL"
     gene            1039..1731
                     /gene="tsaB"
                     /locus_tag="VFN42_05470"
     CDS             1039..1731
                     /gene="tsaB"
                     /locus_tag="VFN42_05470"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET8996099.1"
                     /translation="MTAEERTRSLRRILALDSAQARCSAGIIQDGRILAEQQETLQRG
                     QAARLPVMLQAVLHAAGLPAAAFDLIAVTIGPGSFTGIRAGLALAHGLALATARPLVA
                     VSAGEALAEMLPPLDGRMLWSAIDSRRGRIFLERGPDIASFGLEALPDPSGPVAVTGD
                     AAGAVAARLAARGHDVLLTDARLPTPLGIALAALRRADGTLPPRLAQPLYVDAPEVRL
                     PAGGLRPPPAAA"
     gene            1728..>2015
                     /locus_tag="VFN42_05475"
     CDS             1728..>2015
                     /locus_tag="VFN42_05475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8996100.1"
                     /translation="MSWSIGLAGPAQAEELAAIHCTAFPPREAWGSDAFALQLILPGT
                     FGLFDPQGGLLLARIAADQAEVLTLAVLPGLRRQGLGRALLRAAMAEAHHRG"
BASE COUNT          290 a          729 c          677 g          319 t
ORIGIN      
        1 gagccacacg acgcaagaag ccgatgcttt agctcgactt cctgcatttt cgggtgcgag
       61 tgcgcatcca tgacgctggt tcctgctgaa ttctgattgt ccaacgaacc cgccaaccct
      121 gccccgaacc ccctcccgca aggcagggaa atcgcatttg gcccgctacc ggcggccgac
      181 agccaaggtg agaacgccct gctctggagc gaaccgccgc gaagcaagat taaaagcttc
      241 gccctgctaa gccgcgaatc gcaccaaacc gggcgttggt ccccgcgctg ccgcccaatc
      301 cgatggcaag gggagggaga gttcctttgt ctcgcttcct cctcgagctc ccttccagcg
      361 cgcgcgcgct tgaccccggc cctgggctgc cccattccgg cttcccgcta ggcattccag
      421 gagacctgca tgacgatcga tgcggccgga ctcgacctgc tgttccgcga ggcgcggagc
      481 cacaacaagt tcaccgacga gccaatcagc gacgagacgc tgcgtgagct ttacgccatt
      541 gtgaagatgg ggccgacctc ggcgaacagt tcgcccgcgc gtttcctgtt cctgcgcagc
      601 aaggaggcga aggagcggct ggccccggcg ctgtcgtccg gcaacctgga aaagaccatg
      661 gcggcgccgg tgacggtgat cgtggcctac gatcccaaat tccacgagaa gctgccgcag
      721 ctcttcccgc ataacccgga cgcccagtcc tggttcacca gcaatgattc gctggccgcc
      781 acgacggcct tccgcaacgg cacgctgcaa ggggcctacc tgatcctggc ggcgcgcgcc
      841 ctcgggctgg ataccgggcc aatgtccggc ttcgacaacg ccaaggtgga cgaggaattc
      901 ctctcctggc gcggctggcg gtcaaacttc ctggtgaata tcggccacgg cgatccatcc
      961 ggcgtgttcc cgcgctctcc ccggctggag ttcgacgaag cctgtgacct gctgtgaccg
     1021 gccggggctg accggggcat gacggcggag gagaggacgc ggtccctgcg ccgcatcctc
     1081 gcgctcgatt cggcgcaggc ccgctgctcg gccggcatca ttcaggacgg ccgcatcctg
     1141 gcggagcagc aggagacgtt gcagcgcggc caggccgcaa ggctgccggt gatgctgcaa
     1201 gccgtgttgc acgccgccgg attgcccgcc gcggcgttcg acctgatcgc ggtcaccatc
     1261 gggccgggca gcttcaccgg catccgcgcc ggcctggcgc tggcgcacgg cttggcgctg
     1321 gcaaccgcgc gtccgctggt cgccgtcagt gccggcgaag ccctggcgga aatgctgccg
     1381 ccgctggacg gccgcatgct ctggtcggcg atcgacagcc gccgcggccg gatcttcctg
     1441 gaacgcggcc cggacatcgc cagcttcggc ctggaggcgc tgcccgatcc gtccggtccg
     1501 gttgcggtga ccggcgacgc cgctggcgcg gtggcggcgc ggctcgcggc gcgcggacac
     1561 gacgtcctgc tgaccgatgc gcggctgccc acccctcttg gtattgccct ggccgcgctg
     1621 cggcgtgccg acggcacgct gccaccccgc ttggcgcagc cgctttacgt tgatgcgccg
     1681 gaggtccgcc tgccggcggg cggcctccgc ccacctcccg ctgccgcgtg agctggtcga
     1741 tcggcctcgc cggaccggcc caggcggagg aattggcggc gatccactgc accgccttcc
     1801 cgccgcgcga ggcctggggg tccgacgcct tcgcgcttca gctcatcctg ccgggcacct
     1861 tcggcctgtt cgacccgcaa ggcgggctgc tgctggcgcg cattgccgcc gaccaggcgg
     1921 aggtgttgac gctggcggtg ctgccggggc tgcggcgcca gggactggga cgcgcgctgc
     1981 tgcgggccgc catggccgag gcgcaccacc gcggc
//
LOCUS       DASSZH010000256         2072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_858145, whole genome shotgun sequence.
ACCESSION   DASSZH010000256 DASSZH010000000
VERSION     DASSZH010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858145"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="VFN42_05480"
     CDS             complement(<1..907)
                     /locus_tag="VFN42_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HET8996101.1"
                     /translation="MTVDPATSPHRAEHDGHAFHLCSAGCRSKFIADPRRYLAPLPPA
                     APAMGAAVASGTIYTCPMHPQIRQAGPGTCPICGMALEPLQATAEAGTNPELADMRRR
                     FWIGLALTIPVVLLEMGGHVPGLGLHGMIAPRLSAWIQFVLATPVVLWAGWPFFARGW
                     ASLVTRNLNMFTLIALGTGAAWLYSVVAVLAPAAFPAGFRGMDGTVAVYFEAAAVITV
                     LVLLGQVLELRAREQTGGAIRALLNLAPKTARRVLADGSDEEIPLERIAVGDRLRVRP
                     GDGVPVDGVVLDGRSAVDESMVTGES"
     gene            1023..1340
                     /locus_tag="VFN42_05485"
     CDS             1023..1340
                     /locus_tag="VFN42_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HET8996102.1"
                     /translation="MALAPYTPHRYGINMHEATRKAVHGRLRRIEGQVGGLLRMVQED
                     RYCVDVLTQINAVRAALHKVEQEILRDHVAHCVANVFASGDVLDQRQKVEELVDTVGR
                     MTR"
     gene            1806..>2072
                     /locus_tag="VFN42_05490"
     CDS             1806..>2072
                     /locus_tag="VFN42_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HET8996103.1"
                     /translation="MRSILLSRRGLLGASAAGIAAFSHRPRAFAASPPLRLTAGLRTL
                     EVNGKSASVFGITGPDGTPGLVIAPGQRFAVTLANRAGEDTIVHW"
BASE COUNT          328 a          728 c          692 g          324 t
ORIGIN      
        1 tggactcgcc ggtgaccatc gattcgtcga cggcgctgcg tccgtccagc acgacaccgt
       61 cgaccggcac cccgtcgccc ggacgaaccc gcaaccggtc gccgacggcg atccgctcca
      121 gcgggatttc ctcgtctgat ccatctgcaa gcacccgccg cgcggtcttg ggggcgagat
      181 tgagcagcgc gcggatcgca ccgccggtct gctcgcgcgc acgcagctcc agcacctggc
      241 cgagcagcac cagaaccgtg atgacggcgg ccgcctcgaa atacaccgcg acggtgccat
      301 ccatgccgcg gaagccagcg gggaaggcgg ccggggcgag cacggcaacc acgctgtaca
      361 gccaggccgc accggtgccg agcgcgatca gggtgaacat gttgagattg cgcgtaacca
      421 aagaggccca gccgcgcgcg aagaacggcc agccggccca gagcaccacg ggcgtcgcga
      481 gcacgaactg gatccatgcc gaaagacggg gcgcgatcat gccgtgcagg ccaagccccg
      541 gtacatggcc acccatctcc agcagcacga ccgggatcgt gagcgcgagc ccgatccaga
      601 atcgccggcg catgtcggcc agctcgggat tcgtgccggc ctcagcagtg gcctgcaacg
      661 gctccagggc cataccgcag atcgggcagg tgcccggtcc ggcctgccgg atctgcgggt
      721 gcatcgggca ggtatagatc gtgcccgagg ccacagccgc gcccattgcc ggggcggcgg
      781 gcggaagcgg cgccaggtag cgccgcgggt cggcgatgaa cttgctgcgg cagccggccg
      841 agcataaatg gaacgcgtgg ccgtcatgct ccgccctgtg cggcgaggtg gccggatcga
      901 cggtcatgcc gcagaccggg tcggtgaccc tttccggggc cggcgccgcc acggcgtcgt
      961 catgcctgtg tccgctcatc agtccctcct cgcacccgcg cctacaaggc gggagtgctt
     1021 cgatggcgct tgcaccttat accccccata ggtatggtat caacatgcat gaagcgacga
     1081 gaaaagcggt tcatggccgt ctgcgccgga tcgaaggtca ggtcggcggc ctgttgcgca
     1141 tggtgcagga ggatcgctac tgcgtggatg tcttgacgca gatcaatgcg gtgcgcgctg
     1201 ccctgcacaa ggttgagcag gaaattctcc gcgaccatgt cgcgcattgc gtcgctaacg
     1261 tcttcgcgtc cggcgacgtg ctggaccagc ggcagaaggt cgaggaactg gtcgatacgg
     1321 tcggccgcat gacgaggtag cggtgttgct gcgaggctgt gcgcagaggt ggttgcgggg
     1381 actggtggca ctgctggtgc tgggcggtgt gctgctcgca ggccagtcca gaacagccgc
     1441 cgccgcgcat cacgccgcct ctggctacgc cttgttgatc gcggcaggcg taagtcatgg
     1501 ggtaccctcg ccctgcccgt ctgacgagcc acacggcata ccctgctgcc agacgagcgc
     1561 gtgcagcatg gtcgtcgcgc cgccccagat cgccgccgcg gtgaccccgc cggtcgcggg
     1621 gagcgttgtt gctttcctcc tgctcgcgcc ttgccgcccc ggcggcacca tccccgatcc
     1681 agcactgcgg ccaccccgcg ccgcgatctg aaccgccggc acgctttcca gcgtccggcg
     1741 ggaggcaagc cgcgtgatcg ccatcgcgac cgcgccattc tcgcttcact cggatcggac
     1801 gcattatgcg cagtattctt ctttcccgac gcggcttgct cggcgcgagc gccgctggaa
     1861 ttgccgcctt ctcccaccgt ccacgcgcct tcgccgcttc gccgccgctt cgcctgaccg
     1921 ccggcctccg taccctggag gtgaacggca agtccgcttc ggtgttcggc atcaccgggc
     1981 cggacggcac gcccggcctt gtgatcgccc ctggccaaag gttcgccgtc acacttgcga
     2041 accgggccgg cgaggacacc atcgtccact gg
//
LOCUS       DASSZH010000257         4561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1301407, whole genome shotgun sequence.
ACCESSION   DASSZH010000257 DASSZH010000000
VERSION     DASSZH010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301407"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1555
                     /locus_tag="VFN42_05495"
     CDS             <1..1555
                     /locus_tag="VFN42_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3458 domain-containing protein"
                     /protein_id="HET8996104.1"
                     /translation="GPLAHPVQPDHYIAIDNFYTATVYNKGAEVILMMATIIGRAAFR
                     RGMDLYFARHDNQAVTIDDFLQAMQDASGVDLQRFKRWYHQAGTPIVSVSDSYDAATR
                     TYTLTLKQHTPPTPGQPDKQPVVIPVALGLLAPGGEELPTRLAGEAQPQSGTRVVLLE
                     AAQQDFVFQDVPAPPVPSLLRGFSAPVRLQGVSQDRMRFLAAHDTDPFVRWESGQQYA
                     TALLLSQIARWRDGKAPETDRALIDGLAATLALGETGDAAFAAEALALPSETFLADQM
                     PVADIDGIHAVREAMRTAIGRALQPALRAAYDRLDDPGEYRIDGASIGRRALRNTCLA
                     YLAAAGGEGARLAKAQFDASRNMTDVLAALAVLANADGPEREEALSAFHARWRQDALV
                     LDKWFAIQAMAKRPGTLDAVRALARHPDYDLRNPNRVRALVGSFAGGNPVAFHDAGGG
                     GYRFLADTILALDPQNPQVAARMVSPLGQWRRVDPARQALMQAELRRILDTPRVSRNT
                     GEMVSRSLA"
     gene            1668..2669
                     /locus_tag="VFN42_05500"
     CDS             1668..2669
                     /locus_tag="VFN42_05500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8996105.1"
                     /translation="MTDTNPRIAAVIPLYNGGRFIRDALESVLAQTLMPSEIIVVNDG
                     STDDGPAIVEAMAQQHPITLLHKENGGQSSARNHGIAHSTAEVIALLDQDDVWYANHL
                     ERMIVPFRRQRIPELGWVYSNLDEIDEAGNIVVRRALDGVVAEHPKRSLYECIAHDMF
                     VLPSATLMSRRAFDAVGGFDERLSGFEDDDLFLRMFRKGFDNIYIPRPLSQWRIFGTS
                     ASFSPRMRRSRMIFFRKLVEACPNDPPRSRYPAMQYLAPRFFPWLVREYTEAIKLGDR
                     AAITAACDDLAYAAALHNPRVRTLMNLALPVMRRPGLALRVMPLIGMIRPTVRRMLR"
     gene            complement(2842..3750)
                     /locus_tag="VFN42_05505"
     CDS             complement(2842..3750)
                     /locus_tag="VFN42_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HET8996106.1"
                     /translation="MPNYDFVTVDVFTEQRFGGNPLAVFPDARGLSDADMQAIAAEFN
                     LSETTFVLPPDNPQHHARVRIFTPRAELPFAGHPNVGTGFVLAQRDPKPPEHYVFEEK
                     AGLVRVHVLRGPDKAITGSRISAPRSLSVDIGIPDDTIARCIGVEVSDVLTTAHTPLV
                     ASVGTPFVIAELRSVAVLSRASPDLAAFRDAAERFSDAGTRFSLHLYARVDGSATHLR
                     TRMFAPLGGILEDPATGSANAALAALLTSLAPGGDVDLTYEIEQGVEMGRPSQIIASA
                     RKTPEGPVLATVAGSCVPVLRGSITL"
     gene            complement(3816..>4561)
                     /locus_tag="VFN42_05510"
     CDS             complement(3816..>4561)
                     /locus_tag="VFN42_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET8996107.1"
                     /translation="EDTDKALRAAIAKFRQERGNKLRGIVLDLRNNPGGLLDQAVDVA
                     DDFLNKGTIVSTHGRHAVDDHKWTASSGDITNGLPVVVLINNGSASASEIVTGALQDN
                     HRALVLGTRSFGKGSVQTLFPLQGNGAIRLTTARYYTPNGFSIQGWGITPNVVVNETD
                     KPVSAFGPEHEADLNNTLKVGAKPPTPPSVPMAAGIPAKPPANWPKYDPTKPDTDFQL
                     QQALKLVTAMANAGAHGTMVAPATGKRPI"
BASE COUNT          688 a         1585 c         1555 g          733 t
ORIGIN      
        1 gggcccgctg gcgcatccgg tgcagccgga ccactacatc gcgatcgaca atttctacac
       61 cgccacggtg tacaacaagg gcgccgaggt catcctcatg atggccacca tcatcggccg
      121 cgctgccttc cgccgcggca tggacctgta tttcgcccgc cacgacaacc aggccgtcac
      181 catcgacgac ttcttgcagg cgatgcagga cgcctccggc gtcgatctgc agcgcttcaa
      241 gcgctggtac catcaggccg gcaccccaat cgtgtcggtc tccgacagct acgatgccgc
      301 aacccgcacg tacacgctga cgctgaagca gcacacgccg cccacccccg gccagcccga
      361 caagcagccc gtggtgatcc cggtggcgct cggcctgctt gctccgggcg gcgaggaatt
      421 gccgacgcgg ctggccggcg aagcgcagcc gcagtccggc acgcgcgtgg tgctgctgga
      481 agccgcgcag caggacttcg tcttccagga cgtgccggcg ccgccggtgc cctccttgtt
      541 gcgtggtttc tccgcaccgg tgcggctgca aggcgtgtcg caggaccgca tgcgcttcct
      601 cgccgcgcac gataccgacc cctttgtgcg ctgggagtcc ggccagcaat acgccaccgc
      661 attgctgctg tcgcagatcg cccgctggcg ggacggcaag gcgccggaga cggaccgggc
      721 cctgatcgac gggttggccg ccacgctggc gctgggcgag accggcgatg ccgccttcgc
      781 cgccgaagcg ctggcgctgc ccagcgagac cttcctggca gaccagatgc cggtagctga
      841 catcgacggt atccacgcgg tgcgcgaagc aatgcggacg gcgatcggcc gcgcactgca
      901 accggcattg cgcgcggcct acgaccggct ggacgatccg ggcgagtacc gcatcgacgg
      961 cgcttcgatc ggccggcggg cgctgcgcaa cacctgcctc gcctacctgg cggcggccgg
     1021 cggtgaaggg gcgcggctgg ccaaggcgca gttcgatgcc agccgcaata tgaccgacgt
     1081 gcttgccgcg ctggcggtgc tggccaatgc ggatggtccg gagcgcgagg aggcgctgtc
     1141 ggcgttccac gcccggtggc ggcaggatgc gctggtgctg gacaagtggt tcgccatcca
     1201 ggcgatggca aagcgccccg gcacgctgga cgcggtgcgt gccctggcgc gccatccgga
     1261 ctacgacctg cgcaacccca accgggtccg cgccctggtc ggcagctttg ccggcggcaa
     1321 tccggtggcg ttccacgacg cgggcggcgg tggctaccgg ttcctggcgg atacgatcct
     1381 ggcgttggat ccgcagaatc cgcaggtggc ggcgcgcatg gtttccccgc tggggcagtg
     1441 gcgccgggtc gatccggcac gccaggcgct gatgcaggcg gagttgcggc ggatcctgga
     1501 taccccgcgc gtgtcgcgca acaccggcga gatggtcagc cgcagcctgg cctgacgccg
     1561 gtcctgggac tgcctggctg gcatgccggg ccgcggcgac aggaggcaat tgcccctgcc
     1621 gcagcggccc agtattttcc cgcgctcaca cccagcatcg gatttgcatg accgacacga
     1681 acccgcggat tgccgccgtg atcccgctgt acaacggtgg caggttcatt cgcgatgccc
     1741 tggaaagcgt gctggcgcaa acgctgatgc cgtcggagat catcgtggtg aacgatggct
     1801 cgaccgacga tggcccggcg atcgtcgagg cgatggccca gcagcatccg atcaccctgc
     1861 tgcacaagga aaacggcggc cagtcctcgg cccgcaatca tgggatcgcc cattccaccg
     1921 ccgaggtgat tgcgctgctg gaccaggatg acgtgtggta cgccaaccac ctggaacgca
     1981 tgatcgtgcc gttccggcgg cagcgcatcc cggaacttgg ctgggtctac agcaacctgg
     2041 atgagatcga cgaggccggc aacatcgtgg tccgccgcgc gctggacggc gtggtggcgg
     2101 agcacccgaa gcgcagcctg tacgagtgca tcgcgcacga catgttcgtg ctgccctccg
     2161 ccacgctgat gtcgcgccgc gcgttcgacg ccgtgggcgg gttcgacgaa cggctgtcgg
     2221 ggttcgagga cgacgacctg ttcctgcgca tgttccgcaa gggcttcgac aacatctaca
     2281 tcccgcgccc gctgtcgcag tggcgcattt tcggcaccag cgcatccttc tcgccgcgca
     2341 tgcgccggag ccggatgatc ttcttccgca agctggtgga agcctgcccg aacgatccgc
     2401 cgcgcagccg ctatccggcc atgcaatacc tggcgccgcg gttctttccc tggctggtgc
     2461 gcgagtacac cgaagccatc aagctcggcg accgcgcggc gatcaccgcg gcctgcgacg
     2521 acttggccta tgccgccgcc ctgcacaatc cgcgcgtgcg cacgctgatg aatctggccc
     2581 tgccggtgat gcgccggccc ggactggcgc tgcgggtcat gccgctgatc ggcatgatcc
     2641 gcccgaccgt gcggcggatg ctgcggtaat cccagccggt cgtctgatga tccagcgcgg
     2701 tgacgcgaac tcccctgtcg tggccgggcc tgatccggcc gcccacgcag gtcctggcgt
     2761 gttccgttgc atcccgaccc ggtcgttccc cggtagatgg ccgggacagg cccggccacc
     2821 acgttgagga gcgccttcgg ctcagagcgt gatcgaaccc ctcagcaccg gcacgcagct
     2881 tcccgccacc gtcgccagca ccgggccttc cggcgtcttg cgtgccgagg cgatgatctg
     2941 gctgggccgt cccatctcca cgccctgctc gatctcgtag gtgagatcga catcgccgcc
     3001 cggcgccagc gaagtcagca gggccgccag cgcggcgttg gcgctgccgg tcgccgggtc
     3061 ctccaggatg ccgccgagcg gggcgaacat gcgcgtgcgg agatgcgtgg cactgccgtc
     3121 gacccgggca tacaggtgca gcgagaaccg cgtcccggca tccgagaagc gctccgccgc
     3181 gtcgcggaac gccgccagat ccggactggc gcgcgacaga accgccacgc tgcgcagttc
     3241 ggcaatgacg aagggcgtgc ccaccgacgc gaccagcggc gtgtgcgcgg tggtcagcac
     3301 gtcggacacc tccacgccga tgcagcgcgc gatcgtgtcg tccgggatgc cgatatccac
     3361 cgacagcgag cgcggcgcgg aaatccggct gccggtgatg gccttgtccg ggccgcgcag
     3421 cacgtgcacg cgcaccaggc cggccttctc ctcgaacaca tagtgttccg gcggcttggg
     3481 atcgcgctgc gccagcacga agccggtgcc cacgttcgga tgcccggcga acggcaattc
     3541 ggcgcgcggg gtgaagatgc gcacgcgcgc gtggtgctgc ggattgtcgg gcggcagcac
     3601 gaacgtggtc tcggacaggt tgaactcggc ggcgatggcc tgcatgtcgg catcgcttaa
     3661 cccccgcgcg tcgggaaaca cggcgagcgg attgccgccg aaacgctgct ccgtgaagac
     3721 gtccacggtg acgaaatcat agttcggcat gggcgcgatc cttcagcgac gggaagcggc
     3781 gccgcctcca cgcggcggaa gcggcgccgc ggcgatcaga tcggccgctt gccggtggcc
     3841 ggcgcgacca tcgtgccgtg cgcacccgca ttcgccatcg cggtgaccag cttcagcgcc
     3901 tgctgtagct ggaagtccgt gtcgggcttg gtggggtcgt atttcggcca attggccggc
     3961 ggcttcgcgg ggatgcccgc ggccatcggc accgagggcg gcgtcggcgg cttggccccg
     4021 accttcagcg tgttgttcag gtcggcctca tgctccggcc cgaatgcgct caccggcttg
     4081 tcggtctcgt tgaccaccac attgggcgtg atgccccaac cctggatgga gaagccgttc
     4141 ggcgtgtagt agcgcgccgt ggtgagccgg atcgcgccgt tgccctgcag cgggaagagc
     4201 gtctgcaccg agcccttgcc gaagctgcgg gtgcccagca ccagcgctcg gtgattgtcc
     4261 tgcagcgcgc cggtgacgat ctcgctggcc gaggcggagc cgttgttgat cagcaccacg
     4321 accggcaggc cattggttat gtcgccagaa cttgccgtcc acttgtggtc gtccaccgca
     4381 tgccttccgt gggtggacac aatagtgccc ttgttcagga agtcatccgc cacgtccacc
     4441 gcctgatcga gcaggccgcc cggattgttg cgcaggtcca gcacgatacc ccgcagtttg
     4501 ttgccgcgtt cctggcggaa cttggcgatc gcggcgcgca gcgccttgtc ggtatcctcg
     4561 t
//
LOCUS       DASSZH010000258         6921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_261661, whole genome shotgun sequence.
ACCESSION   DASSZH010000258 DASSZH010000000
VERSION     DASSZH010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6921
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261661"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..464)
                     /locus_tag="VFN42_05515"
     CDS             complement(102..464)
                     /locus_tag="VFN42_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HET8996108.1"
                     /translation="MAPLQAAPLSVAALRCRCPRCGQGPLYTGLLTVREHCTVCGLDL
                     RQADTGDGPAVFVMFFLSIVVIGLGVWVEFTFSPPLWVHVVLWPLPTLALAIAVMRPA
                     KALMVALQYRTRASEMGL"
     gene            complement(468..1322)
                     /locus_tag="VFN42_05520"
     CDS             complement(468..1322)
                     /locus_tag="VFN42_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HET8996109.1"
                     /translation="MDTDTHAASTLAVSSGLKQPYHLVDPSPWPLVGAVGGGLTVLGI
                     VLAAHYGNYAVLALGLIVVLATMFFWWRDVLKESATAGLHSPVVRLGLRYGMTLFIAS
                     EVMFFVAFFWAYFHFALYPEHVAGDTAGVWPPPGIHTFDPFHTPFLNTLILLLSGTTI
                     TWAHHALIENNRRQLVLGLGLTVLLGLSFTGFQIMEYSSAPFHFVGGGVYPSVFFLAT
                     GFHGFHVIVGTCFLIVCWFRARAGSFTPQRHFGFEAAAWYWHFVDVVWLFLFVCIYWW
                     GAGQIAME"
     gene            complement(1378..2016)
                     /locus_tag="VFN42_05525"
     CDS             complement(1378..2016)
                     /locus_tag="VFN42_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET8996110.1"
                     /translation="MPYPPEHRQETRSRILDAARRLFNRRGLTDVSIDEVMEAAGLTR
                     GAFYSYFPSKEALYAEAITHFIDGARLAPWQCGQGGPWPQGEALARRILDAYLSDGHF
                     ADLDGSCPLIGLAADVARSEGPARGAYRQVLGVMTGAFAAHFGPSVLDDGANLPEQRA
                     LAVAAMCVGGMVLARAVDDAALAGALRAAAHRYAVALAGWDAPEAVDVAPAP"
     gene            2131..2496
                     /locus_tag="VFN42_05530"
     CDS             2131..2496
                     /locus_tag="VFN42_05530"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8996111.1"
                     /translation="MANPFVHVELNTTDLPRAKQFFASLFDWDLAEMPMGDGMTYTAI
                     RVGEGTGGGMMTHPMPGQPSIWIPYVLVPDVAAATEKARGLGAQVIRGKTEVPDMGWF
                     SVILDPTGAALGMWQTKMA"
     gene            complement(2506..3096)
                     /locus_tag="VFN42_05535"
     CDS             complement(2506..3096)
                     /locus_tag="VFN42_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HET8996112.1"
                     /translation="MTKRKPGRALPITLAAVVGGMLGLSFAAVPLYRVFCAVTGYGGT
                     PQIGPEVSKEVSERTITVRFNADVNPNLPWSFAPEQKQVTLKLGEQQVAFYHARNDSD
                     HPITGVAVYNVTPDKIGTYFHKTACFCFNEQTLAPHQEMQLPVSFWVDPAIAKDPSTA
                     DVTVITLSYTFFRSLDDADKSGKLATAGPHVGPLVR"
     gene            complement(3108..3269)
                     /locus_tag="VFN42_05540"
     CDS             complement(3108..3269)
                     /locus_tag="VFN42_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996113.1"
                     /translation="MPADRPPAPPNDDAALARRRRGRNIAMLVALLALVLLFYAIAMV
                     KMAHHGLPH"
     gene            complement(3275..4246)
                     /locus_tag="VFN42_05545"
     CDS             complement(3275..4246)
                     /locus_tag="VFN42_05545"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HET8996114.1"
                     /translation="MDQRAAGLLAGGSGDRASPLAFSGTTALDDSSARDWLILLKPRV
                     VALVVFTGLVGLLVAPGHLHPVLAFTAVLSIAVAAGAAGAINMWYDRDIDALMRRTAN
                     RPIPAGRIEPGQALGFGLTLAAGSVLVMGLATNLVAAGILALSIAFYVGVYTMWLKRR
                     TPQNIVIGGAAGAFPPVIGWAAVTGSVDVLPLLLFAIVFFWTPPHFWSLSLWAHGDYQ
                     RAGVPMLPVVAGARATRRQIMLYTLLLAPLAVLPWGLGLTGPVYGLSATVLGLAFVAG
                     AWRVLFDKQDEAGRSLTRDAPARALFRYSLLYLAVLFAALAVDRLVG"
     gene            complement(4259..5857)
                     /gene="ctaD"
                     /locus_tag="VFN42_05550"
     CDS             complement(4259..5857)
                     /gene="ctaD"
                     /locus_tag="VFN42_05550"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HET8996115.1"
                     /translation="MSVTTHDHAHHDDHHGHAPGFVARWLFSTNHKDIGTLYLIFAVC
                     GGTVGLILSLIMRLELFSPGMHHFASGQEWNTVVTAHGLLMIFFAVMPALIGGFGNWF
                     VPIMIGAPDMAFPRLNNISFWLLVPAFILVVSGLLMGESGSGWTLYPPLSNATYEPGP
                     GMDFTLFALHLAGISSLLGAINFITTIFNMRAPGMTLHKMPLFVWSILVTAFLLLLSV
                     PVLAGAITMLITDRNFGTAFFDPQGGGDPVLFQHLFWFFGHPEVYIMILPGFGIISHV
                     VSTFSKKPVFGYLGMAYAMVAIGVVGFVVWAHHMFVSGIGVDTRAYFMAATMIIAVPT
                     GIKIFSWIATMWDGSIELKVPMLWAVGFIFVFTVGGVTGVVLSNAGLDQILHNTYYVV
                     AHFHYVLSLGAVFSIFAGFYYWIGKMSGRPYPEFWAKVHFWLTFVGVNLTFFPMHFLG
                     LAGMPRRYVDYPEAFTGWNQVATVGSFISGLGMLVFFYVCYRTFASKEHLAENYWGEG
                     ATTLEWSLSSPPPFHTFEELPRIR"
     gene            complement(5904..6752)
                     /gene="coxB"
                     /locus_tag="VFN42_05555"
     CDS             complement(5904..6752)
                     /gene="coxB"
                     /locus_tag="VFN42_05555"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HET8996116.1"
                     /translation="MQLLRRLAAAFAFTLGGVLASAGPVHAQLPRPWEMGMQVAHSPV
                     QARIESLHTLVLWIITLVTLFVGALLVWVLFRYNARANPTPGRTTHHTLLEVAWTVVP
                     VLILAIIVIPSFRLVYYEDRTASADMTVKVTGHQWYWEYAYPDQNNIDFTSYIVKDAD
                     LKPGQPRLLTVDNPLVVPAGKNIRILATSSDVIHSFFIPSLGVQRYAIPGRMIETWFR
                     ADQPGEYHGECNQICGTNHSFMPIVIKAVTPEEFQAWVKDAKTRFAANPPEDPSRFAA
                     ASAQVQ"
BASE COUNT         1304 a         2336 c         2335 g          946 t
ORIGIN      
        1 gccacgtccc caggccgagc agcaccagca gcatgacgaa ggtgctgacc gccggcacca
       61 gaaggcggcg cccgcccatc gcgctcacca ttccgtcccg ctcacaaccc catctcgctc
      121 gcgcgggtgc ggtattgcag cgccaccatc agcgccttgg ccggccgcat caccgcgatc
      181 gcgagcgcca gcgtggggag cggccagagg acgacatgga cccagagcgg cggcgagaag
      241 gtgaactcca cccacacgcc caggccgatc acgacgatgc tgaggaagaa catgacgaac
      301 acggccggcc cgtcgcccgt gtccgcctgt cgcaggtcga ggccgcagac cgtgcagtgc
      361 tcccgcacgg tcagcaggcc cgtatagagc ggcccctggc cgcagcgcgg gcagcggcag
      421 cgcagcgccg ccacggagag cggcgctgcc tgcagcggtg ccacgcgcta ctccatcgcg
      481 atctggccgg cgccccacca gtagatgcag acgaacagga acagccatac cacgtcgacg
      541 aagtgccagt accaggcggc ggcctcgaag ccgaaatgcc gctgcggggt gaagctgccg
      601 gcgcgggcgc ggaaccagca gacgattagg aagcaggtgc cgacgatgac gtggaagccg
      661 tggaacccgg tcgccaggaa gaacaccgac ggatagacgc cgccgcccac gaaatggaac
      721 ggggcgctgg aatactccat gatctggaag ccggtgaacg acagcccgag cagcaccgtc
      781 agcccgaggc cgaggaccag ctggcggcga ttattctcga tcagggcgtg atgcgcccag
      841 gtgatcgtgg tgcccgagag cagcaggatc agcgtgttca ggaacggggt gtggaacgga
      901 tcgaaggtgt ggatgcccgg cggcggccag acgccggcgg tgtcgcccgc cacatgctcg
      961 ggatacagcg cgaagtgaaa ataggcccag aagaaggcga cgaagaacat cacctcgctg
     1021 gcgatgaaca gcgtcatgcc gtagcgcagg cccagccgca cgaccggcga atggaggccg
     1081 gcggtggcac tctccttcag cacgtcgcgc caccagaaga acatcgtggc cagcacgacg
     1141 atgaggccga gcgccagcac ggcatagttg ccgtagtgcg cggccagcac gattcccaga
     1201 acggtcaatc cgccaccgac cgcgccgacg agcggccacg ggctcggatc gaccaggtga
     1261 taaggctgct tgaggcccga gcttacagcc aaggtgctcg cggcgtgcgt atcggtgtcc
     1321 atgcccctgc ccatcggaaa aaaacctggg cgcatcatcc ccaaaatgcc gcgcgcttca
     1381 aggggccggc gccacatcga ccgcctccgg cgcatcccag ccggccagcg caaccgcata
     1441 gcgatgcgcc gccgcgcgca acgccccggc aagtgccgca tcatccaccg cgcgcgccag
     1501 caccatacca ccgacgcaca tcgcggcgac agccaatgcc cgctgctccg gcagattggc
     1561 gccgtcgtcc agcacgctcg gcccgaaatg cgcggcgaag gcgccggtca tcacccccag
     1621 cacctgccgg taggcgccgc gcgccggtcc ctcgctgcgc gccacgtcgg ccgcgagccc
     1681 gatcagcggg catgacccgt cgagatcggc gaaatgccca tccgagagat aggcgtcgag
     1741 gatgcgccgc gccagcgcct cgccctgcgg ccacggcccg ccctgcccgc actgccaggg
     1801 ggccagccgt gcgccgtcga tgaaatgcgt gatcgcctcg gcatacagcg cctccttcga
     1861 cgggaagtag ctgtagaagg cgccgcgggt caggccggcg gcttccatca cctcgtcgat
     1921 cgagacatcg gtcaggccgc ggcggttgaa cagccgccgc gcggcatcga ggatgcggct
     1981 tcgggtctcc tgccggtgtt cgggtggata cggcatggcg gcgtgcggtc cctctctgtt
     2041 cccggatgtt cagaatatgt tcttgaacat atcctgccca gcccgcaccg tccccccact
     2101 tcgcgtgaca ccagccagga ggagcggcac atggccaacc cgttcgtgca tgtcgagctg
     2161 aacacgaccg atctgcccag ggcgaagcag ttcttcgcgt ccctgttcga ctgggacctc
     2221 gcggagatgc cgatgggcga cggcatgacc tatacggcga tccgcgtcgg cgagggcacc
     2281 gggggcggca tgatgacgca tcccatgccg ggccagccct cgatctggat cccctacgtg
     2341 ctggtgcccg acgtggcggc ggcaacggag aaggcgcgcg gcctcggcgc gcaggtgatc
     2401 cgcgggaaga cggaggtgcc ggacatgggc tggttctccg tcatcctcga cccgaccggc
     2461 gccgcgctgg ggatgtggca gacgaagatg gcctgaggaa cgcgcctacc gcaccagggg
     2521 gccgacatgc ggcccggcgg tcgccagctt gccgctcttg tccgcgtcgt cgagcgagcg
     2581 gaagaaggtg taggacagcg tgatcacggt cacgtcggcg gtggacgggt ccttcgcgat
     2641 cgccggatcg acccagaagc tgaccggcag ctgcatctcc tggtgcgggg cgagcgtctg
     2701 ctcgttgaag cagaaacagg cggtcttgtg gaaatacgtg ccgatcttgt ccggcgtcac
     2761 gttgtagacg gcaacgccgg tgatcggatg atcggagtcg ttgcgggcgt gatagaaggc
     2821 cacctgctgc tcgccgagct tcagcgtcac ctgcttctgc tccggcgcga aggaccaggg
     2881 caggttcggg ttcacgtcgg cgttgaagcg cacggtgatg gtgcgctcgc tgacctcctt
     2941 gctgacctcc ggcccgatct gcggcgtgcc gccatagccg gtgacggcgc agaacacgcg
     3001 atacagcggc accgcggcga aggagaggcc gagcatcccg ccgaccaccg cggcgagcgt
     3061 gatcggcagg gcgcggcccg gcttgcgctt ggtcatggca aagcccctca gtgcggcagg
     3121 ccgtgatgcg ccatcttcac catcgcgatg gcgtagaaca gcagcaccag cgccagcagc
     3181 gccacgagca tggcgatgtt gcgcccgcga cgacggcgcg cgagcgccgc gtcgtcgttt
     3241 gggggtgcag gggggcgatc agccggcatc acgctcatcc gaccagccgg tccaccgcga
     3301 gggccgcgaa cagcaccgcg agatagagca gcgagtagcg gaacagcgcc ctggccggcg
     3361 catcccgcgt caggctgcgc ccggcctcgt cctgcttgtc gaacagcacg cgccaggcgc
     3421 cggccacgaa cgccagcccc agcacggtcg ccgacagccc atagaccggc ccggtgagac
     3481 cgagccccca gggcagcacg gccagcggtg ccagcagcag cgtgtagagc atgatctgcc
     3541 gccttgtggc ccgcgcgccg gccaccaccg gcagcatcgg caccccggcg cgctggtagt
     3601 cgccatgcgc ccacagcgac agcgaccaga aatgcggcgg cgtccagaag aacacgatgg
     3661 cgaacagcag cagcggcagc acgtccacgc tgccggtcac cgccgcccag ccgatcaccg
     3721 gcgggaaggc gccggcggcg ccgccgatga cgatgttctg cggcgtgcgc cgcttcagcc
     3781 acatcgtgta gacgcctacg tagaaggcga tcgacagggc caggatgccg gccgcgacca
     3841 ggttggtcgc cagccccatc accagcaccg acccggccgc gagcgtcagc ccgaagccga
     3901 gcgcctgccc cggctcgatc cgcccggcgg ggatcggccg gttggccgtg cggcgcatca
     3961 gggcgtcgat atcgcggtcg taccacatgt tgatggcacc cgccgccccc gccgccaccg
     4021 cgatcgacag cacggcggtg aaggccagca ccgggtgcag gtgccccggc gccaccagca
     4081 gcccgaccag cccggtgaac accaccaggg ccacgacgcg cggcttcagc aggatcagcc
     4141 agtccctggc cgatgagtca tcgagagcgg tggtgccgga aaaggcgagt ggggacgccc
     4201 ggtccccact gccccccgcc agcaacccgg cggcacgctg gtccatcttg ttcctcgttc
     4261 agcggatccg cggcagttcc tcgaaggtat ggaacggcgg cggggaggaa aggctccatt
     4321 caagagtcgt ggcgccctcg ccccagtagt tctcggccag atgctccttc gaggcgaagg
     4381 tgcggtagca cacgtagaag aacaccagca tgcccaggcc cgagatgaac gacccgaccg
     4441 tcgccacctg gttccagccc gtgaacgcct ccggatagtc gacgtagcgg cgcggcatgc
     4501 cggcgaggcc gaggaagtgc atcgggaaga aggtcaggtt cacgccgacg aaggtgagcc
     4561 agaaatgcac cttggcccag aactccggat acgggcggcc ggacatcttg ccgatccagt
     4621 agtagaaacc ggcgaagatc gagaacaccg cgcccagcga cagcacgtag tggaagtggg
     4681 ccaccacgta gtacgtgttg tgcaggatct ggtcgagacc ggcgttcgac agcaccacgc
     4741 cggtcacgcc gccgaccgtg aacacgaaga tgaagccgac cgcccagagc atcggcacct
     4801 tcagctcgat cgacccgtcc cacatggtcg cgatccagga gaagatcttg atgccggtcg
     4861 gcacggcgat gatcatcgtc gcggccatga aataggcgcg cgtgtcgacg ccgatgccgg
     4921 agacgaacat gtggtgcgcc cacaccacga acccgaccac gccgatcgcc accatcgcat
     4981 aggccatgcc gagatagccg aacaccggct tcttcgagaa ggtggagacg acgtggctga
     5041 tgatgccgaa gcccggcagg atcatgatgt agacttcggg atggccgaag aaccagaaca
     5101 ggtgctggaa cagcaccggg tcgccgccgc cctgcgggtc gaagaaggcg gtgccgaaat
     5161 tgcggtcggt gatcagcatc gtgatcgcgc cggccagcac cggcaccgac agcagcagca
     5221 ggaacgccgt caccaggatc gaccacacga acagcggcat cttgtgcagc gtcatgccgg
     5281 gcgcgcgcat gttgaagatc gtggtgatga agttgatcgc gccgagcagc gaggaaatgc
     5341 ccgcgaggtg cagcgcgaac agcgtgaagt ccatgcccgg cccgggctcg taggtcgcgt
     5401 tcgacagcgg cggatacagc gtccagccgg atcccgactc gcccatcagc aggcccgaga
     5461 cgaccaggat gaaggccggc accagcagcc agaagctgat gttgttcagg cgcgggaagg
     5521 ccatgtcggg cgcgccgatc atgatcggca cgaaccagtt gccgaacccg ccgatcagcg
     5581 ccggcatcac ggcgaagaag atcatcagca ggccgtgcgc cgtgaccacc gtgttccatt
     5641 cctgcccgga ggcgaaatgg tgcatgcccg gcgagaacag ttcgagccgc atgatcagcg
     5701 acaggatcag cccgaccgtg ccgccgcaca cggcgaagat caggtagagc gtgccgatgt
     5761 ccttgtggtt ggtgctgaac agccaccgcg cgacgaagcc cggcgcgtgg ccgtggtgat
     5821 cgtcgtggtg ggcgtggtca tgggtggtga cactcatcct ctgtctcctg cgcgcggcgg
     5881 acgggcgccc ggtccgccgg cggtcactga acttgcgcgg acgcggcggc gaagcgcgac
     5941 ggatcttccg gcgggttggc cgcgaacctg gtcttcgcgt ccttcaccca ggcctggaac
     6001 tcctccggcg tcaccgcctt gatcacgatc ggcatgaagc tgtggttggt gccgcagatc
     6061 tggttgcact cgccgtgata ctcgcccggc tggtcggcgc ggaaccaggt ctcgatcatg
     6121 cggccgggaa tcgcgtagcg ctgcacgccc aggctcggga tgaagaagct gtggatcacg
     6181 tccgaactgg tcgccaggat gcggatgttc ttgccggccg gcaccaccag cggattgtcc
     6241 accgtcagca gccgcggctg gcccggcttc aggtccgcgt ccttcacgat atagctcgtg
     6301 aagtcgatat tgttctgatc cggatacgca tattcccagt accactgatg cccggtgacc
     6361 ttcaccgtca tgtcggcgct ggcggtacgg tcctcgtagt agacgaggcg gaagctcggg
     6421 atgacgatga tcgccaggat cagcaccggc accacggtcc aggccacttc cagcagcgtg
     6481 tggtgggtgg tgcggcccgg cgtcgggttg gcgcgggcgt tgtagcggaa cagcacccag
     6541 accagcagcg cgccgacgaa cagcgtgacc agcgtgatga tccacagcac cagcgtgtgc
     6601 agcgactcga tgcgcgcctg cacggggctg tgggcgacct gcatgcccat ttcccacggg
     6661 cgcggcagct gtgcatgcac ggggccggcc gaggccagca ccccgccgag ggtgaacgca
     6721 aatgcggcag caagccgccg gagtagctgc atcggccgat ccatcccgtt gaggacgccc
     6781 aggaccatat cgtcctgcgc tgcagcaact tggctacatg aggccctgcc tcagatcaag
     6841 gcccccgcga cgacacattc gagatttgca atacgagaca gcggctcagg cggccagttc
     6901 gaccagggtg aacaggccga a
//
LOCUS       DASSZH010000259         2120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1250316, whole genome shotgun sequence.
ACCESSION   DASSZH010000259 DASSZH010000000
VERSION     DASSZH010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250316"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1367
                     /locus_tag="VFN42_05560"
     CDS             <1..1367
                     /locus_tag="VFN42_05560"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET8996117.1"
                     /translation="HVLLALELPTPDGGAAVQRYFRLMAGHAVDVASGRPAGPVHLNF
                     PFREPLVPLAPIGAPACPTPEGHPFVAPGPAGREPAPEQTAAVADLLRRARRGLIVCG
                     GQDDPAFPAALVQLAVRAQFPVLADPLSGVRCGPHAADIVVDSYDAFLRDAALAETMQ
                     PDLVLRFGAAPTSKPLLQYLERHAKCPQLLIADGWWSDPALIAAAALDGDPTACCRRL
                     LDMLAGHRPVASALAWSRRWEQADAVSRAVLADGAAGFAEPFEGRVFGELAHLLPDDS
                     VLFVGNSMPVRDLETFFPRGRRRVRFLANRGASGIDGVVSTALGAAAGGRSAVLVIGD
                     LSFYHDMNGLLAARQHHLDLTIVLINNDGGGIFSFLPQAGQVEHFERLFGTPHGLEFA
                     PVAALYGARYTLSQSWAHFAAAVQEGIEQGGLHIVEVRTEREQNVRQHRALWQHVAEA
                     LRQA"
     gene            1364..>2120
                     /gene="menH"
                     /locus_tag="VFN42_05565"
     CDS             1364..>2120
                     /gene="menH"
                     /locus_tag="VFN42_05565"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR03695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HET8996118.1"
                     /translation="MKRRLTVNGLGLNVERQGIGAPLVLLHGFTGSTASWGTCMPELA
                     QRFTVYAVDLVGHGRTEAPDDLTHYRMPTAVADLAALLDALELERVALLGYSMGGRTA
                     LRFAVAYPERVRTLILESASPGIADPLERATRRRSDEALADHIERDGLATFVREWEQL
                     PLFRSQARLPAAVRAAQRDQRLQCNPRGLANSLRGMGAGVQEPVWDALAQLSMPVRLI
                     TGQEDDKYGALARSMAERLRDARLAIVAEAGHAV"
BASE COUNT          286 a          714 c          752 g          368 t
ORIGIN      
        1 agcatgtcct gctggctctg gaactgccga cgccggatgg cggcgcggcc gtccagcgct
       61 acttccgctt gatggccggg cacgctgtcg atgtcgcgtc gggccggccg gccgggccgg
      121 tccatctcaa ctttcccttc cgtgagccgc tggtgccgtt ggcgccaata ggggcgcccg
      181 cttgtcccac gcctgaaggc catcccttcg tcgcccccgg gccggctggg cgagagcccg
      241 cgcccgaaca gaccgccgcc gtggccgatt tgctcaggcg cgcgaggcga ggcttgatcg
      301 tctgcggcgg gcaagatgac ccggcctttc cggccgccct ggtgcaactt gctgtgcggg
      361 cgcagttccc ggtactcgcg gatccgctgt ccggtgtccg ctgcggcccc cacgctgccg
      421 acattgtcgt ggacagctac gacgcgttcc tacgcgacgc cgccttggcc gagaccatgc
      481 aacccgacct tgttctccgc tttggcgccg ccccgacatc aaagccgctc ctgcagtacc
      541 tggagcgcca cgccaaatgt ccgcaactgc tcattgccga tggctggtgg agtgacccgg
      601 ccctgatcgc ggcggcggct ctggacggcg atcccaccgc ctgctgccgg cgcctgctgg
      661 acatgctcgc tggccaccgt ccggtggcct cggcgctcgc ttggtcccgg cgctgggaac
      721 aggcggatgc ggtttctcgt gccgtcctgg cggacggcgc tgccggcttc gccgagccgt
      781 tcgaaggccg ggtgttcggg gagcttgccc acctgttgcc cgacgattcc gtcctctttg
      841 tcggcaacag catgccggtg cgggatctgg agaccttctt tccgcgcggc cggcgtcgcg
      901 ttcgtttcct ggccaaccgg ggggccagcg gcatcgacgg tgtggtctcg acagctctag
      961 gtgctgccgc cggcggccgt tcggccgtgc tggtgatcgg tgacctttcc ttttaccacg
     1021 acatgaacgg actgctggcg gcgcgccagc accacctgga cctgacgatc gtgctgatca
     1081 acaacgatgg cggcggcatc ttctcgttcc tgccgcaggc tggccaggtg gagcacttcg
     1141 agcgcctctt cggcacgccg cacggcctag agttcgcgcc ggtggcggcg ctctatggcg
     1201 cgcgctacac gctgtcccag tcctgggcgc actttgccgc cgccgtgcag gaggggatcg
     1261 aacagggagg gctgcacatc gtggaggtac ggacggaacg ggagcagaac gtgcgccagc
     1321 atcgcgcgct gtggcaacac gtggcggaag cgctgcgaca ggcgtgaaaa ggcgactcac
     1381 cgtcaatggc ctcggcctca acgtcgagcg acaggggatc ggcgctccgc tggtgttgct
     1441 gcacggcttc accggtagca ccgcttcctg ggggacctgc atgccggagt tggcccagcg
     1501 gttcacggtc tatgccgtcg atctcgtcgg ccacggaagg acagaggcac cggatgacct
     1561 gacccattat cggatgccga ccgccgtggc cgacctggcg gcgctcttgg atgcgctgga
     1621 gctggagcgc gtggcgctgc ttggctactc catgggcggg cgcacggccc ttcggttcgc
     1681 cgtcgcctat ccagagcgcg tgcggacgct gatcctggag agcgcctcgc cgggcatcgc
     1741 cgacccgctg gagcgggcga cgcggcgccg cagcgacgaa gcgctggcgg accacatcga
     1801 gcgggacggc ctggcgacgt ttgtgcggga gtgggagcag ttgccgctct tccgttcgca
     1861 ggcgcgcctg ccggcagcgg tgcgggcggc acagcgggac cagcggctcc agtgcaaccc
     1921 gcgcggacta gccaacagcc tgcgcggcat gggggcaggc gtacaagagc cggtttggga
     1981 tgccttagcg caattgtcga tgccggttcg gctgatcacc ggccaggagg acgacaagta
     2041 tggtgcgttg gcgcgttcca tggcggagcg cctgcgagat gcccggttgg caatcgtggc
     2101 ggaggcggga catgcggtac
//
LOCUS       DASSZH010000260         4162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_329793, whole genome shotgun sequence.
ACCESSION   DASSZH010000260 DASSZH010000000
VERSION     DASSZH010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329793"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            380..1129
                     /locus_tag="VFN42_05570"
     CDS             380..1129
                     /locus_tag="VFN42_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8996119.1"
                     /translation="MSDRLANKTAFVTAAGQGIGRATALAFAAAGARVIATDINEALV
                     QQIVSDRIHAAKLDVRQPEAIAQAARDAGPVDILFNCAGYVHQGTLLEATEDEWSFAF
                     DLNVRAMFRTMRAFLPGMLQRGGGVILNMASVASSLKGVPNRFVYSASKAAVIGMTRS
                     VATDYVKQGIRCNCLCPGTVHTPSLDERIAANAAAAGSADAALAAFVARQPMGRLGSP
                     EEIADLAVYLAGDSAQFITGQALVIDGGLTL"
     gene            1182..1481
                     /locus_tag="VFN42_05575"
     CDS             1182..1481
                     /locus_tag="VFN42_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996120.1"
                     /translation="MGVETSRSGPDMVRRLALERPVFPTYYSPMDDTPHRQPPADWEP
                     PMPSEAELRASLARSDADIAAGRTVPASAVHAELQAALDRIEARRPRERSAAARR"
     gene            1571..1774
                     /locus_tag="VFN42_05580"
     CDS             1571..1774
                     /locus_tag="VFN42_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996121.1"
                     /translation="MRALAEAEAEIERDPGAGLAAPRPYPQLSRPGRAWIKAGRYWIV
                     YTTTRPPVIAGVFYDAANIPGRL"
     gene            1896..2918
                     /locus_tag="VFN42_05585"
     CDS             1896..2918
                     /locus_tag="VFN42_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HET8996122.1"
                     /translation="MTQTATTRRFWVLKRRPAGEPTRDDFDLREDAIPQPGPGEVLTR
                     TIFLSIDPYMRGRLREQQTYAAAVQIGEMMTGETVGEVVASAHPDFVPGDVAVGARGW
                     ASHLLTRGEQLVKVPGHAAPLSAWLGVLGMPGATAYAGISEICKPQPGETVVVSAASG
                     AVGSIAGQLAKRAGARVVGVAGGAEKCLWVQDALGFDDCVDHRSLNLEDAVAAACPKG
                     VDAYFENVGGAVQAAVFDLLNPFARVAMCGMVSQYNSEHLPPGPNLGFVVGKRVLIQG
                     FIVSDRPERLVEWRRLAAPWVAEGSLRYREDVVGGLEQAPDALAGILTGRNFGKLLVR
                     VGEDPS"
     gene            3018..3749
                     /locus_tag="VFN42_05590"
     CDS             3018..3749
                     /locus_tag="VFN42_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996123.1"
                     /translation="MHTVKYRLFEPRLAPRRTKMEVPGWSGKPEPRRDGSHEFAWHCM
                     PFVEGAQYGIELFYPFDNELRVTKRNGHVVLEAEWGPDPDTGVNWPPFRSFGEGYYTY
                     QLLLDLKVTDDMAVLAQPHSRYYTAENDDVPLAVPALLRTSWWPMISFVVFKAPPEGH
                     YHVFRPGEPFMQLLFVPADPEFQLVLMDEEEAAEREMRGRRIHASRETLGKATTWTSS
                     TNTVFDATYRQILRAARAKAKQADD"
BASE COUNT          681 a         1370 c         1424 g          687 t
ORIGIN      
        1 gtttgtagcg gtcgaggaaa ccaccttcat gccgagacag ccactggcat ccgcgctccg
       61 caattttgtg ggccttgcgg actcgatcat cgggctgttc gacacctaac ccggattctg
      121 gcgaacggaa aagggcaccc atagacgcat aatagcggaa gataggaact ttcacacggc
      181 gatagaccat tgatctaaaa cgtttctctg cttttctttc tgagtaatta ggcaaaaaat
      241 cttacttcgc cgtgccgcga atgcccccgc ggccgagcgg cgcaggggcg gccttcatgc
      301 ggacacgagc tgagccgtgc tgccttccag cccatccggc cctccggcat aatcgagcgt
      361 ctcatctggg atttgccgca tgtccgaccg cctggccaac aagaccgctt tcgtcaccgc
      421 cgccggccag ggaatcgggc gggcgaccgc gctggccttc gccgccgccg gcgcgcgggt
      481 gatcgcgacc gacatcaatg aagcgctggt gcagcagatc gtatcggacc gcatccacgc
      541 cgcgaagctc gacgtgcggc agccggaggc gatcgcacag gccgcgcgtg atgccggccc
      601 ggtggatatc ctgttcaact gcgccggcta tgtgcaccag ggcacgctgc tggaggcgac
      661 cgaggacgaa tggagcttcg ccttcgacct caacgtgcgc gccatgttcc gcaccatgcg
      721 cgcattcctg ccgggcatgc tgcagcgcgg cggcggcgtg atcctgaaca tggcctcggt
      781 cgcgtccagc ctgaagggcg tgccgaaccg cttcgtctac agcgccagca aggctgccgt
      841 gatcggcatg acgcgctctg tggcaaccga ctatgtgaag caggggatcc gctgcaattg
      901 cctgtgcccc ggcacggtac acacgccgag cctggacgag cggattgcgg cgaatgccgc
      961 ggcggcgggg tcggcggatg cggcgctggc ggccttcgtg gcgcgccagc cgatggggcg
     1021 gcttggctcg ccggaggaga tcgccgacct ggcggtgtac ctggccggcg attcggcaca
     1081 gttcatcacc ggccaggcgt tggtgatcga tggcgggctg acgctgtagg cccagctttc
     1141 cttggggtgc tccacgtgcg cccccatcgt ggggaaggtg gatgggcgtt gagacaagcc
     1201 gcagcggtcc ggacatggtc cggcggctgg cgctggaacg tccggtcttc ccgacctatt
     1261 attcccccat ggacgacaca ccgcaccgcc agccgcctgc cgattgggag ccgccgatgc
     1321 ccagcgaggc cgagctacgt gcctcgctcg cccgtagcga tgcagatatt gccgcagggc
     1381 gcactgtgcc cgcctcggct gttcacgcag agctgcaagc cgcgcttgac cggatcgagg
     1441 cacggcggcc gcgagagcgc agcgccgccg cgcggcgttg attgaatacg ctgacgaggc
     1501 tctacagcac gtccgcactc tccgccgtca ctatgaggcc ttggaccgag acaacgccat
     1561 ccggtcgctc atgcgagcac tggccgaggc cgaggcagag attgaacgtg atcccggcgc
     1621 aggactcgcc gcgccgcgcc cctatcccca gctttcccgg ccggggcggg cgtggatcaa
     1681 ggccgggcgg tattggatcg tctacacgac aacccgcccg ccggtgatcg ccggcgtgtt
     1741 ctacgacgcc gcgaacattc ctggtcgcct gtagcgcgcc cgacgcgtgc cgccggctag
     1801 ggccgacact tgtcccgtgg gaagcctcgc atccggcccc ctgtgccctt ggccaacaat
     1861 gcgccacact ccggcagcat gaaccggagc cagccatgac ccaaaccgcg acaacccgcc
     1921 gcttctgggt gctgaagcgc cgccccgccg gcgaacccac ccgggacgac ttcgacctgc
     1981 gcgaggatgc catcccgcag cccggtccgg gcgaggtgct gacccgcact atcttcctgt
     2041 ccatcgatcc ctacatgcgc ggccggctgc gcgaacagca gacctacgcc gccgcggtgc
     2101 agatcggcga aatgatgacc ggggaaacgg tgggcgaggt ggtcgcctcg gcgcatccgg
     2161 acttcgtccc cggcgacgtc gcggttggcg cgcgcggctg ggcctcgcac ctgctgacgc
     2221 ggggcgagca actggtgaaa gtgcccggcc atgcggcgcc gctctccgcc tggctcggcg
     2281 tgctcggtat gccaggcgcc accgcctatg ccgggatcag cgagatctgc aagccgcaac
     2341 cgggggagac cgtggtggtg tccgctgcct ccggcgcggt cggctcgatc gccgggcagc
     2401 tcgccaagcg ggccggcgcg cgcgtcgtgg gcgtggctgg cggggccgag aagtgcctgt
     2461 gggtgcagga cgccctggga tttgacgact gcgtcgatca ccgttcgttg aacctggagg
     2521 atgcagtcgc cgcggcctgc cccaagggcg tggacgccta tttcgagaat gtcggcggcg
     2581 cggtgcaggc cgccgtgttc gacctgctga acccattcgc gcgtgtcgcg atgtgcggca
     2641 tggtgtcgca atacaactcg gagcacctgc cgccgggacc gaatctgggc ttcgtggtgg
     2701 gcaagcgcgt gctgatccag ggcttcatcg tgtcggaccg gccggagcgg ctggtggaat
     2761 ggcggcggct tgccgcgccc tgggtggccg agggcagcct gcggtatcgc gaggacgtgg
     2821 tcggcgggct ggagcaggca cccgatgcgc tggccggcat cctcaccgga cgcaatttcg
     2881 gcaagctgct ggtccgcgtg ggcgaggatc cgtcctgacg gcagcgcatc gaaccgggcg
     2941 ggaaccggca acccgggcct gcgttggtgt tcgctgcacc atcgaacata tctctgcggc
     3001 gagcgaggga gttccgcatg cacacggtca agtatcggct gtttgaaccg cgtctggcgc
     3061 cgcggcgcac caagatggaa gtgcctggct ggtccggcaa gccggagccg cggcgtgacg
     3121 gctcgcacga attcgcctgg cactgcatgc cgttcgtcga aggcgcgcaa tacggcatcg
     3181 aactgttcta tccgttcgac aacgaattgc gcgtgaccaa gcgcaacggg catgtggtgc
     3241 tggaggcgga gtggggaccg gacccggaca ccggcgtcaa ctggccgcca ttccgctcgt
     3301 tcggggaggg ctactacacc tatcagctct tgctcgacct gaaggtgacc gatgacatgg
     3361 cggtgctggc gcagccgcac tcgcgctact acaccgctga aaacgacgac gtgccgctgg
     3421 cggtgccggc gctgctgcgc accagctggt ggccgatgat ctccttcgtg gtgttcaagg
     3481 cgccgccgga ggggcactat cacgtgttcc ggcccggcga acccttcatg cagctcttgt
     3541 tcgttccggc cgacccggaa ttccaactgg tgctgatgga tgaggaagaa gcagcggaac
     3601 gcgagatgcg cggccggcgc atccacgcca gccgggaaac cctgggcaag gctaccacct
     3661 ggacgtcgtc aaccaacacg gtgttcgacg ccacctaccg gcagatcctg cgtgcggcac
     3721 gtgccaaggc gaagcaggcg gacgattgag ctggcgcggg cgctgacctg agcctccacg
     3781 ccccggcggg cgaaggcgcg ccacccacgc tttgttggcg aagacccatg cgccgcaagc
     3841 cgcgtaggaa gtctgccaca gcactgggtg ggtggcaggc cttcccctgc cacaacgctg
     3901 ttcggctatg ggaaaatcca aaacggcttg cgacgtgact ctcctgcggc aagtcacgca
     3961 tggcgggccg gcgcgcttac tgggcgccgg cccgccacgg atccacgccg cctcaatcgc
     4021 ggcggtcgtt atgcaccacg aaaccgccga caccgggaat gccgacgtag ctgttgtggt
     4081 gatggtggtg atggtggtgg tcgccaccgc ggttgtagta gccgtagtag ttgtcgtagc
     4141 ggcgatgatg gtgatgatgg tg
//
LOCUS       DASSZH010000261         7118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_346833, whole genome shotgun sequence.
ACCESSION   DASSZH010000261 DASSZH010000000
VERSION     DASSZH010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7118
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346833"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VFN42_05595"
     rRNA            complement(<1..339)
                     /locus_tag="VFN42_05595"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(801..1793)
                     /locus_tag="VFN42_05600"
     CDS             complement(801..1793)
                     /locus_tag="VFN42_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET8996124.1"
                     /translation="MSILVTGAAGFIGSHVAQALLGRGEQVIGVDNLNTYYDVRLKQA
                     RLDRLAPERGFAFHQLDVSDREGMAALAAANPGITAIVHLAAQAGVRHSLVDPYAYVQ
                     SNVMGHVVVQEVARRLPALRHLVYASTSSVYGLNQQMPFREADRVDTPASLYAATKRA
                     DELMAHAYAHLFSLPQTGLRFFTVYGPWGRPDMAYYSFARAMLAGEPITLFDDGRLRR
                     DFTYIEDIVAGVLGCIERPPQGAPPLRVLNIGNNQPEEVRTLVALLEQGLGRKAVVRS
                     APRPPADVEATFADITAIEALTGFAPRTSLAEGIGRFCAWFLAFHGQAGTPQTF"
     gene            complement(1790..2383)
                     /locus_tag="VFN42_05605"
     CDS             complement(1790..2383)
                     /locus_tag="VFN42_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET8996125.1"
                     /translation="MTEAERFTAMMHRSAELKRRIAIELAPAVVAVVAACEAGLRGGG
                     KLLFCGNGGSAADSQHLATELLIRLRSSVERDSWPALALTLDPAMLTACGNDYGYDRV
                     FERPLRGLGRRGDVLFAITTSGRSPSVLRALQAAREMGIVTVGLLGGAGDPAGSLCDH
                     VLLVPDSETARIQECHIALGHAVLELLEDRLVAAPLR"
     gene            complement(2465..4075)
                     /locus_tag="VFN42_05610"
     CDS             complement(2465..4075)
                     /locus_tag="VFN42_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HET8996126.1"
                     /translation="MNPPIHADYVVIGAGSAGCVVAARLSETGAQVVLLEAGPRDWLP
                     WIHIPAGVLKLLAHPVVNWNYAAAAGPGTAGRTIHWPRGKVLGGSSSINGMLYVRGNP
                     ADFDGWAQMGCRGWSFDDVLPYFRKSEHYVQGGDPEYRGQGGPLKVEDYRTILPLTHR
                     FVEAAQEAGFPFSRDLNGREQEGVGYSQMTRRGRFRGSTARTFLAEARRRPNLRVETE
                     ALASRLIFDGRRCVGAAFRQQGQHREVRVTREVILCGGAVNSPHLLQISGIGPADHLQ
                     TIGVPVVHHLPGVGANLQDHYVARVAHRARNAVSINELSRGLRLAAEAVRFALPGNGA
                     LTFGVTSAQVFTRSREGLASPDLQLLFTPASYDMGKFGTLEREAGMTVAICPVRPGSR
                     GTIMAKASDPAAAPAINPNYLSDPNDQRVLLSGIAITRRIFAQPAIAQHSALETLPGP
                     QVQTEDALAEYCRRTCTTIFHPVGTCRMGEGPAAVVDPRLRVHGIDGLRVIDASVMPT
                     LTTGNTNAPTIMIAEKGAAMIREDAHAA"
     gene            complement(4126..5598)
                     /gene="zwf"
                     /locus_tag="VFN42_05615"
     CDS             complement(4126..5598)
                     /gene="zwf"
                     /locus_tag="VFN42_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET8996127.1"
                     /translation="MARIIPVAVFDCVVFGATGDLTLRKLLPALYYRFRDGQMPPESR
                     VIGAARSDIGEEGFRGRARGALEEHVAPADLDPDTVDRFCARLHYAHLDASRPGDHWR
                     ALQQLLDPERVRVFYLATSPELYGPICRNLGSAQLVTPESRVVLEKPIGHDLESARRI
                     NDEVGAVFSEAQIFRIDHFLGKETVQNLLALRFANTIFERLWTADDIDHVQITVAETV
                     GLEGRAGYYDRSGALRDMVQNHLLQLLCLIAMEPPVSLDADRVRDEKLKVLRALCPIE
                     PHDAGTLTVRGQYAKGAIGGRPVPGYLEDLGDGGTSGTETFVALKAEVRTWRWAKVPF
                     YLRTGKRLPQKVSEIVVQFRPLPFSIFPPDSAEWRANRLIIRLQPEEGMRLEMMTKDP
                     GPGGLRLSPTGLDIRFASTFGVRFPDAYERLLMDTVRGNPTLFMRRDEVEAAWAWVEP
                     ILQAWESRPDQPRPYAAGSWGPTAAIALIERDGRTWHEEI"
     gene            5785..6867
                     /locus_tag="VFN42_05620"
     CDS             5785..6867
                     /locus_tag="VFN42_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HET8996128.1"
                     /translation="MPEFVVPPAQPASRVLPAAREALTTAGRLLPGLLLAIGVTLAAD
                     LLQGVEARLFGRPWIEALVLAILLGTAIRTAWLPSRRWAPGIHFAAKPVLEFAVVLLG
                     ASVSAQTVAAVGPGLLVGIPAVVALALGAGYLIGRVFRLPHRMAVLVACGNAICGNSA
                     IAAVAPVIGAEGGDIASSIAFTAVLGVLVVLILPLLMPLMHLSTAQFGVLAGLTVYAV
                     PQVLAATAPVAASSVQIGTLVKLMRVLMLGPVVLALSLAAGSEARASGIAVRRRLHRL
                     VPWFIIGFLAMAALRSLALLPPLVLQPLSSAAGLLTVVSMAALGLGVDVRTVARAGVR
                     VASAVTLSLLLIGGISLALIRLIGVG"
     gene            complement(6830..>7118)
                     /locus_tag="VFN42_05625"
     CDS             complement(6830..>7118)
                     /locus_tag="VFN42_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET8996129.1"
                     /translation="SQGLSLAAVRVAMELPSNEAVCAAVEAGGGATVISASVAAPGLE
                     AGLLHLVRFPVPERAFRVLRHRDRPQSNAAAALLDMVAGVSRPRSTGSEPD"
BASE COUNT         1153 a         2533 c         2253 g         1179 t
ORIGIN      
        1 tccccactgc tgcctcccgt aggagtctgg gccgtgtctc agtcccagtg tggctgatca
       61 tcctctcaga ccagctaccg atcggagcct tggtaggccg ttaccccacc aacaagctaa
      121 tcggacgcag gctcctcctc aggcgacttg cgcctttaga cctcagtcgt catgcggtat
      181 tagccacagt ttcccgtggt tgtcccccac ccgaggatag atacctacgc gttactcacc
      241 cgtccgccac tgcctgaggt tgcccccaaa ccgtgcgact tgcatgtgtt aagcatgccg
      301 ccagcgttcg ctctgagcca ggatcaaact ctcaggttca tctcagcccc aggtctcccc
      361 agaacttcaa cgaacagacc cgaactcgtc gatccatctt ggcctcgcga ccaacatgaa
      421 accatcgaag cgtattctgt cacgtatgca gagatacatc acagcaaacc cctcaacagc
      481 ccaaaccaac aagcccaggt tcaaaaaacg ccaacccaaa ccgtcagaac aaaccatcca
      541 atcagccacc cactcgcgca ggtaacccat agggcgccgc caacgtatcc cttcctagcc
      601 tattcacctg tcaaagaacg gagcaaaaac gcccaaccac ccgcacttct acgggccggc
      661 cggccgtctt gctctgagtt gcacccttcc cggtgcagac cggagagagt agcgaaaccg
      721 cggcggcgaa gcaagtgttt tcgtccgctg cggtgaaggg gatttaggcc agccgttccc
      781 cccggtcaag caggtatttt tcagaaagtt tgcggagtcc ccgcctggcc gtggaaggcc
      841 aggaaccagg cgcagaagcg gccaatcccc tcagccaggc tggtgcgcgg cgcaaagccg
      901 gtcagcgctt cgatcgccgt gatatccgcg aaggtcgcct ccacatctgc aggtgggcgg
      961 ggcgcgctgc gaaccaccgc cttgcggccc agcccctgct ccagcagcgc caccagggtg
     1021 cgcacttcct cgggctggtt gttgccgata ttcagcaccc gcagcggcgg tgcgccttgc
     1081 ggcggccgtt cgatgcagcc caacacgccg gccacgatat cctcgatata ggtgaaatcc
     1141 cgccgcagcc ggccgtcatc gaacagggtg atcggctcgc cagccagcat tgcccgggcg
     1201 aagctgtaat acgccatgtc cggccggccc cacggaccgt acaccgtgaa aaaccgcaga
     1261 ccggtctgcg gcaggctgaa cagatgcgca taggcgtgcg ccattaattc gtcggcccgc
     1321 ttggtcgcgg cgtacagcga ggccggggtg tccacccggt ccgcttcccg gaacggcatc
     1381 tgctggttca gcccgtacac cgaggaggtg ctggcataga ccaggtgccg cagcgccggc
     1441 agccgccgcg ccacttcctg caccaccaca tgccccatga cattggactg cacataggca
     1501 tagggatcga ccagcgagtg ccgcacgccc gcctgtgccg ccaggtgcac gatcgctgtg
     1561 atccccgggt tcgcggcggc cagggccgcc atcccttcgc gatccgacac atccagctgg
     1621 tggaaagcga agccgcgctc gggtgccagc cggtccagcc gagcctgctt caggcgcacg
     1681 tcataatagg tgttcaggtt gtccaccccg atcacctgtt cgccccggcc cagcagcgcc
     1741 tgcgccacgt gggagccgat gaagcccgcc gcgccggtga ccaggatgct catcgcagcg
     1801 gcgcagcgac caggcggtcc tccagcagct ccagcaccgc atggccgagg gcaatgtggc
     1861 attcctggat gcgggcggtt tcgctgtccg gcaccagcag gacgtgatcg cacaggctgc
     1921 cggccggatc ccctgccccg cccagcagcc cgaccgtgac gatacccatc tcacgcgccg
     1981 cctgaagggc gcgcagcacg ctgggagagc ggccggaggt ggtaatcgcg aacagcacgt
     2041 cgccacgccg gccgaggccg cgcagcgggc gctcgaacac gcggtcatag ccgtaatcgt
     2101 tgccgcaggc ggtcagcatg gcggggtcca gggtcagcgc cagcgccggc caggaatcgc
     2161 gctcgaccga gctgcgcagg cggatcagca gctccgtcgc caggtgctgg ctgtccgcgg
     2221 ccgaaccacc attgccacag aacagcagct tgccgccgcc gcgcaggccg gcctcacagg
     2281 ccgccaccac tgccaccacc gccggagcga gttcgatcgc gatccgccgt ttcagttcgg
     2341 cagacctgtg catcattgcg gtgaaacgtt cggcctcggt catgcgctca tccgtcgatc
     2401 attactgcgc ttctacaggc ggcgccggcc cgtggccact gaatccccgg ccttccccga
     2461 acggtcatgc cgcatgcgcg tcttcacgga tcatcgcggc ccccttctcc gcgatcatga
     2521 tggtcggggc gttggtgttg ccggtggtca gcgtcggcat caccgaggcg tcgatcaccc
     2581 gcaagccgtc gatcccgtgc acccgcagcc gcggatcgac caccgccgcc ggcccttcgc
     2641 ccatccggca ggtaccgacc ggatggaaaa tcgtggtgca ggtgcgtcgg cagtactcgg
     2701 ccagcgcgtc ctcggtttgc acctgcggac ccggcagtgt ctccagcgcg ctgtgctgcg
     2761 cgatcgccgg ctgggcgaag atccgccggg tgatggcgat gccggacaac agaacgcgct
     2821 ggtcgttcgg gtccgacaga tagttgggat tgatcgccgg cgcggcggcc ggatcggacg
     2881 ccttggccat gatggtgcca cggctgccgg gccgcaccgg gcagatcgcg accgtcatgc
     2941 cggcctcgcg ttccaacgtg ccgaatttgc ccatgtcgta actggccggg gtgaacagca
     3001 attgcaggtc cgggcttgcc agcccctcgc gagagcgggt gaacacctgg gccgaggtca
     3061 caccgaatgt cagtgcgccg ttgcctggca gcgcgaagcg caccgcctcc gccgccaagc
     3121 ggagcccgcg cgacagttcg ttgatcgaaa ccgcgtttct tgcccggtgt gcaacccggg
     3181 cgacataatg gtcctgcaga ttggcgccga cgccgggcag atgatgcacc accggcacgc
     3241 cgatcgtctg caggtggtcg gctggtccga tcccggagat ctgcagcagg tgcggcgagt
     3301 tcaccgcgcc gccgcacagg atcacctcgc gcgtcacccg cacttcgcga tgctggccct
     3361 gctggcggaa cgccgcgccg acgcagcgcc tgccgtcgaa gatcaggcgg gacgcgaggg
     3421 cttccgtttc cacgcgcagg ttgggccggc ggcgcgcctc ggccaggaag gtgcgcgcgg
     3481 tggagccgcg gaaacggcca cggcgcgtca tctgcgagta gccgacgcct tcctgctcgc
     3541 gtccgttcag gtcgcggctg aacggaaaac cggcctcttg cgcggcttcg acgaaccggt
     3601 gcgtcagcgg caggatggtg cggtagtcct ccaccttcaa cggcccacct tgcccgcgat
     3661 actcgggatc gccgccctgc acgtagtgtt ccgatttgcg gaagtacggc agcacgtcat
     3721 cgaagctcca gccgcggcag cccatctgcg cccagccatc gaaatccgcc ggattgccgc
     3781 gcacgtacag catgccgttg atcgagctgg agccgccgag caccttgccg cgcggccagt
     3841 ggatggtgcg gccggcggta cccgggcccg cggctgcggc gtagttccag ttcaccaccg
     3901 gatgggccag cagcttcagc accccggcgg ggatgtggat ccagggcagc cagtcgcgcg
     3961 gccctgcttc aagcagcacg acctgggctc cagtctcact taaccgggcg gcgacgacac
     4021 agccggcgga tccggcaccg atcacgacgt agtccgcatg aatgggtggg ttcatgttcc
     4081 ccactgtgtc acgtcccgtg cggaccagcc aatattggcg ccgagctaga tttcctcgtg
     4141 ccaggtccgc ccgtcgcgct cgatcagcgc gatcgccgcg gtgggtcccc agcttcctgc
     4201 cgcatagggc cgcggctggt ccgggcgact ctcccaggct tgcaggatcg gctccaccca
     4261 ggcccacgcg gcttccacct catcgcgccg catgaacagc gtcgggttgc cgcgcaccgt
     4321 gtccatcagc agccgctcat aggcatcggg gaagcgcacc ccgaaggtgc tcgcgaagcg
     4381 gatatccaac ccggtggggc tcagccgcaa accgccgggg ccgggatcct tggtcatcat
     4441 ctccagccgc atgccttctt ccggctgcag gcggatgatc agccggttgg cgcgccattc
     4501 cgccgaatcc ggcgggaaga tggagaacgg caacgggcgg aactgcacca cgatctctga
     4561 gaccttctgc ggcaaccgct tcccggtgcg caggtagaac ggcaccttcg cccaccgcca
     4621 ggtccgcact tcggctttca gcgccacgaa ggtctcggtt ccgctggtgc cgccgtcgcc
     4681 cagatcctcc aggtaaccgg gtaccggcct gccgccgatc gcccccttgg cgtactggcc
     4741 gcgcacggta agcgtcccgg catcatgcgg ctcgatcgga cacagcgcgc gcagcacctt
     4801 cagcttctcg tcgcgcaccc ggtcggcatc gagcgacacc ggcggctcca tcgcgatcag
     4861 gcaaagcagc tgcagcagat ggttctgcac catgtcgcgc agcgcaccgg aacggtcgta
     4921 atagccggca cggccttcca gcccgaccgt ttccgccacg gtgatctgca cgtggtcaat
     4981 atcgtccgcc gtccacaacc gctcgaagat cgtgttggcg aagcgcagtg ccagcaggtt
     5041 ctgcaccgtc tccttgccga ggaaatggtc gatgcggaag atctgtgcct cgctgaacac
     5101 cgcgccgact tcgtcgttga tccgccgtgc cgattccagg tcgtggccaa tcggcttctc
     5161 cagcacgaca cgggactctg gcgtgacgag ctgcgcactg cccaggttgc ggcagatcgg
     5221 accatacagc tccggggatg tcgccaggta gaacacccgc acccgctccg gatccagcag
     5281 ttgctgcaac gcgcgccagt gatcgcccgg gcgcgacgca tccagatgtg cgtagtgcag
     5341 gcgcgcgcag aagcgatcca ccgtatccgg gtccagatcg gccggggcga cgtgctcctc
     5401 cagcgcgccg cgcgcccggc cgcggaaacc ctcttcgccg atgtcggacc gcgcggcgcc
     5461 gatcacccgc gattccggcg gcatctgccc gtcgcggaac cggtagtaca atgccggcaa
     5521 cagcttgcgc agcgtcaggt cacccgtggc gccaaacacc acgcagtcga acaccgccac
     5581 ggggatgatc ctcgccatcc tggcctcctg cctggaaagc ctccaagtgg gcgctctgcc
     5641 gcagcctaac agaggggggc ctcctgccgc actgctgtgc aaccgatctg aattttcgat
     5701 catacgcaga agttaaatcc gttggaccga tcgaccgtcc aggcccagtt cgggcggcac
     5761 gtgtcacccc actgaggtcc gctcatgcct gaatttgtcg tccctccggc gcaacctgcg
     5821 tcccgagtgc tgccggccgc acgcgaggcg ctcaccacgg cgggccgcct gctccctggc
     5881 ctgctgctcg cgatcggcgt cactctcgcc gccgacctgc tgcaaggggt ggaagcccgg
     5941 ctgtttggcc ggccctggat cgaagccctg gtgctggcca tcctgctcgg caccgccata
     6001 cgcacggcct ggctcccgag ccgacgctgg gcacccggca tccacttcgc tgccaaaccc
     6061 gtattggaat tcgccgtggt gttgctcggg gcctcggtca gcgcccaaac cgtcgccgca
     6121 gtcgggccgg gactgctggt cggcattccc gccgtggtcg ctctggcact cggcgccggg
     6181 tatctgatcg gccgcgtgtt tcgcctgccg catcgcatgg cggtgctggt ggcgtgcggc
     6241 aatgccatct gcggcaactc ggcgatcgcc gcggtcgcac cggtgattgg cgccgagggt
     6301 ggcgacatcg ccagttccat cgccttcacc gcagtgctgg gtgtgcttgt cgtgctgata
     6361 ttgccgctgc tgatgccgct gatgcacctc tccaccgccc agttcggcgt attggccggg
     6421 ctgacggtct atgccgtgcc gcaggtcctg gcggccaccg cacccgttgc cgccagcagc
     6481 gtgcagattg gcacgctggt caagctgatg cgggtgctga tgctcgggcc cgtcgtgctg
     6541 gctctgtcgc tggccgccgg cagcgaggca cgcgcctcgg gtatcgcggt gcggcgcaga
     6601 ctgcaccggc tggtgccgtg gttcatcatc ggcttcctgg ccatggcggc cctgcgctcg
     6661 ctggccttgc tgcctcccct ggtcttgcag ccgctgtcat ccgcggcggg actgctcacc
     6721 gtggtgtcga tggcggcgct ggggctgggg gtggatgtgc gcaccgtggc ccgggccggc
     6781 gttcgcgtgg cgtccgccgt cacgctgtcg ctgctgctga tcggcggcat cagtctggct
     6841 ctgatccggt tgatcggggt cggctgaccc cggcgaccat gtccagcagt gcggcggcgg
     6901 cattgctttg cggccggtcg cgatggcgca gcacgcggaa cgcgcgctcc ggcacgggga
     6961 agcggaccag gtgcagcaac cccgcctcca ggcccggcgc ggcgacggag gcggaaatca
     7021 ctgtggcacc accgccggcc tccactgccg cgcacaccgc ctcgtttgac ggcagttcca
     7081 tggcaacgcg gacggcagcc agcgacaggc cctggctc
//
LOCUS       DASSZH010000262         5860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_926339, whole genome shotgun sequence.
ACCESSION   DASSZH010000262 DASSZH010000000
VERSION     DASSZH010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926339"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..1294
                     /locus_tag="VFN42_05630"
     CDS             239..1294
                     /locus_tag="VFN42_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996130.1"
                     /translation="MPLTSKTYEVSDIHAAQELYHANGWTDGLPIVPPTEDAVQACLD
                     WAMTPAEQLLGIEPVRGVPITAEKLAVNAVMAGCLPMHFPVVLTALSAMLQPEFLLHG
                     ATASTGGCAVLVILNGPIRREIGASGGFNALGNSDRATAVIGRAIRLALINILEVRPG
                     GIDRSTLGHPGKFSYCIAADEEDSEWLPLHVQRGLPPEVSAVTVMAAGAPRQLMNAWT
                     TQPEEILETFAAEMRANMRHYSIWEGNYALVIPKQLRDHLQAAGWSKTDIAACIQQRA
                     RIRRSEWAEVGKGAVVRDRGDSEYSALPSPEHLLVVAAGGPAGGFGAVIPPWLGHKSR
                     AVTLPIGVCIDCEPDSV"
     gene            complement(1336..2085)
                     /locus_tag="VFN42_05635"
     CDS             complement(1336..2085)
                     /locus_tag="VFN42_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8996131.1"
                     /translation="MAEHEADVPRVELRGIGKHYGQGDTRVDALRDIDLSLHAGEIVG
                     LRGPSGSGKSTLLNVVGCVVEPSSGWMRLDGALLYDGRWLRRDLRRLRLLEIGFIFQT
                     HNLLPFLNATENVAEAMELAGATPRNARSRAEALLDYLGVAHRRHAMPSELSGGEAQR
                     VAIARALANSPRIILADEPTAPLDTQRAGAVMDLLHRIAVDQHAAVLVVTHDETIFNR
                     FDRLIALRDGRIEGELHAAPLADAPGQAAPG"
     gene            complement(2078..3220)
                     /locus_tag="VFN42_05640"
     CDS             complement(2078..3220)
                     /locus_tag="VFN42_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8996132.1"
                     /translation="MNLAVRDMRHNAPRFLLTIIGLGMLLGVVTAMVGIYEGALADAL
                     RLPRSENTALWVVQPDTFGPFAEPSRIPRDTRDLVRRMPGVAAAGAVTFQTVQTAVSG
                     RPVRFLLQGYEIGRPGGPSNLIAGHGLTESHYQLVLDASSGLRLGDQVPLGPYRDPYT
                     VVGLTRGMVTSSGDAVGWVTLLDAEALQFAVPPPLQRREAASGRPPMTTADINAVLVH
                     LAPYASVEAVAAEIGRWKHLAAVTERQQETFLTAFVIDKMQRQLGMFMAILVAVSAVI
                     IALIIYTLTMDKLRSIATLKLIGAPDRTIIGLIVQEALALGISSFLLGLGLISLLKDR
                     FPRRVQIEPRDVAILFAIVVLVCLLGSVLAVRAAIRVDPAKALGSG"
     gene            complement(3217..4359)
                     /locus_tag="VFN42_05645"
     CDS             complement(3217..4359)
                     /locus_tag="VFN42_05645"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET8996133.1"
                     /translation="MAGLVLLFAALAGLQLWRAWLHPITVHVAGTASTVPAQVFGLGT
                     VGARVQSNVGFKVAGTLVALHADAGDHVAAGSVLAQLDDRDVLAQRASAHAGVQQAQA
                     NIGKATADVASAEASLFNAEQVSRRRTALARSGVVSVEVAQTNDAAMRVARANVAVAQ
                     SEVAVAQAALAAAQAQEEYATVAADNDTLRAPYDAWVVSRNLQLGSMPVPGQSVFTLV
                     DPTTIWVLGYVDERLAGRVAVGQAATIVLRSDPSRALPGHVARIEIQSDPVNQERLVE
                     VGFDQVPPDIHLAEQAEMFITTDTLRRAVLVPPLAVTHRTGAEGMVWTVESGRLQQRK
                     VTFGPDLLDGRLPIVAGLPDDVPVVISPTAGLIAGRAAVVAQDAGR"
     gene            4615..5139
                     /locus_tag="VFN42_05650"
     CDS             4615..5139
                     /locus_tag="VFN42_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_273701405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:4894..4896,aa:Sec)
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HET8996134.1"
                     /translation="MPLQVLDPTNESAPPLGQSAPRLASLAGKTVGFISNGKEGTKGF
                     FAHLDRMLREEFGVADVVMRVKSNYSAPADPDIVDEIARWHAVVTGIGDUGSCSSCSL
                     HDSITAERLGIPAMAVMTTRFVSAAEMMARVLGFPDYAFAVIAHPVSSAGDAALRDMA
                     AAAIQQARTLLLRG"
     gene            complement(5145..>5860)
                     /locus_tag="VFN42_05655"
     CDS             complement(5145..>5860)
                     /locus_tag="VFN42_05655"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8996135.1"
                     /translation="MVFCTATVMLASSALMAPYLETLAQYPVETAGWSLAPRGLGVMI
                     GMQVASRLSHRIDHRKLMATGLLTMGSALYSISFWTPDVAQHVMMVTLMVQGFGTGLV
                     FNPMTVVAFTTLPSHLRGYATSLQSLCRNLGQAVGVSVTSLMLVRNMQTSHAGIAAQI
                     TPFDRTLQGHDTVSRLLDPATRHGAALLDRLINHQAQIIAFNDDFRMMCFVVIPPLLL
                     LLLMRRHERRPAPAPAPGE"
BASE COUNT          982 a         2062 c         1961 g          855 t
ORIGIN      
        1 atttccggga cgccctccgc gagccgcgcc gcggctcgca ttcaaccagt cgatcgcgat
       61 ctcatgcccg actggcccca tgatgtcata tgggtgaaag tcaccatgac ataggcgatg
      121 gcccttcggc gcgcgacatg agcctgcccc gcactctctg cgccaggctt gacccggtcc
      181 agagcgaggc gaaccatcag tcattcagct tatcccgcgc gcagttgcga gacaccccat
      241 gcccctcacc tccaagacct acgaagtctc cgacatccac gcggcgcagg aactctacca
      301 tgccaacggc tggaccgacg gcctgccgat cgtcccaccc accgaagacg cggtgcaggc
      361 ctgcctcgac tgggccatga ctcccgccga gcagctgctc ggcatcgagc cggtgcgcgg
      421 cgtgccgatc accgcggaga aactcgcggt caacgcggtg atggccggct gcctgccgat
      481 gcacttcccc gtggtgctca ctgcgctttc cgccatgctg cagccggaat tcctgctgca
      541 cggcgccacc gcctccaccg gcggctgcgc ggtgctggtg atcctgaacg gaccgatccg
      601 gcgggagatc ggcgcatccg gcgggttcaa cgcgctgggc aacagcgacc gtgccaccgc
      661 ggtgatcggc cgggcgatcc ggctggcgct gatcaacatc ctggaggtac ggcccggcgg
      721 catcgaccgc tccaccctcg gccacccggg caagttcagc tactgcatcg ccgcggacga
      781 ggaagacagc gaatggctgc cgctgcacgt gcagcgcggc ctgccgccgg aggtgtccgc
      841 cgtcacggtg atggccgccg gcgcgccgcg ccagctcatg aacgcgtgga ccacgcagcc
      901 ggaagagatc ctggaaacct tcgccgccga gatgcgcgcc aacatgcggc actactcgat
      961 ttgggagggc aactacgccc tggtcatccc gaaacagttg cgcgaccacc tgcaggccgc
     1021 cggctggagc aagaccgata tcgccgcctg catccagcag cgcgcgcgga tccgccgcag
     1081 cgaatgggcg gaggtcggca agggcgccgt ggtgcgtgac cgcggcgaca gcgagtacag
     1141 cgccctgccc tcccccgagc acctgctggt ggtcgcggca ggcgggccgg ccggcgggtt
     1201 cggcgcggtg atcccgccct ggctggggca caagagccgc gcggtcaccc tgccgatcgg
     1261 cgtgtgcatc gactgcgaac cggacagcgt gtgacccgcc cggcgcggag ccgcctgcct
     1321 ggcatgcgca cagtgtcagc ccggcgcggc ctgcccggga gcgtcagcca gcggtgccgc
     1381 atgaagctca ccctcaatgc gtccgtcacg cagcgcgatc agccggtcga agcgattgaa
     1441 gatggtctcg tcatgcgtca ccaccaggac ggcggcgtgc tgatcgaccg cgatccgatg
     1501 caacagatcc atcaccgccc ccgcccgctg cgtgtccagc ggcgcggttg gttcatccgc
     1561 caggatgatg cgcggggaat ttgccagggc tcgggcaatc gccacgcgct gggcttcgcc
     1621 gccggatagc tcggacggca tggcatgccg gcggtgggcg acgccgaggt agtccagcaa
     1681 agcctccgcc cggctgcgcg cattgcgcgg cgtcgcgccg gccaattcca tcgcctcggc
     1741 gacgttctcg gtcgcgttga ggaacggcag caggttgtgg gtctggaaga tgaagccgat
     1801 ctccagcagg cgcagccggc gcaggtcgcg gcgcagccat cgcccgtcat agagcagcgc
     1861 gccatccagc cgcatccagc cactgcttgg ctcgacgacg cagccgacga cgttcagcaa
     1921 cgtgctcttt cccgacccgc tcgggccgcg caacccgacg atttcaccgg catgcaggct
     1981 caggtcgata tcgcgcaagg catccacccg cgtgtcgccc tggccgtaat gcttgccgat
     2041 cccgcgcagt tccacgcgcg gcacatcagc ttcgtgctca gccactgccc aatgccttgg
     2101 cgggatccac ccggatggcc gcgcgcaccg ccagcaccga tcccagcagg cagacgagca
     2161 cgacgatggc gaacaggatg gccacgtcgc gcggctcgat ctggacgcgg cgggggaacc
     2221 ggtccttcag cagcgagatc agccccaggc caagcaggaa gctgctgatg cccagcgcca
     2281 gcgcctcctg cacgatcagg ccgatgatcg tccgatccgg cgcaccgatc agcttcagcg
     2341 tggcgatcga gcgcagcttg tccatggtga gcgtgtagat gatcagggcg atgatcaccg
     2401 ccgatacggc aaccaggatg gccatgaaca tgccaagctg ccgctgcatc ttgtcgatca
     2461 cgaaggcggt gaggaacgtc tcctgctgcc gctcggtcac cgcggccaga tgcttccagc
     2521 ggccgatctc ggctgccacg gcttccactg acgcgtaggg tgcgaggtgc accagcaccg
     2581 cattgatgtc ggcggtggtc atcggcgggc ggccggatgc cgcctcccgc cgctgcagcg
     2641 gcggcggtac cgcgaactgc aaggcttcgg catccagcag cgtgacccag ccgaccgcat
     2701 ccccggacga cgtcaccatg ccccgcgtca atccgacgac ggtgtaggga tcgcgatacg
     2761 gccccagcgg cacctggtcg cccagacgca gtcccgacga ggcgtccagc acgagctggt
     2821 aatggctttc ggtcaggcca tggccggcga tcaggttgga cgggccgccg ggacggccga
     2881 tctcatagcc ttgcagcagg aaccgcaccg gccggccgga caccgcggtc tgcacggtct
     2941 ggaacgtcac cgcgccggcc gcggccacgc cgggcatgcg ccgcaccaga tcgcgggtgt
     3001 cgcgcggaat gcgcgagggt tcggcgaagg ggccgaacgt gtccggctgc accacccaca
     3061 gcgcggtgtt ctcggaccgt ggcaggcgca gcgcgtccgc cagcgcacct tcgtagatgc
     3121 cgaccatggc ggtcaccacg cccagcagca tgccaagccc gatgatggtg agcaggaagc
     3181 gcggcgcgtt gtgacgcatg tcgcgaaccg cgaggttcat cgtccggcat cctgcgccac
     3241 cacggcggcg cggccggcaa tcaatccggc ggtcggcgag atcaccaccg gcacatcgtc
     3301 cggcaagccg gcgacgatcg gcaggcgccc gtccagcagg tcgggaccga acgtgacctt
     3361 gcgctgctgt aacctgccgg attccaccgt ccacaccatg ccctcggcgc ccgtgcggtg
     3421 ggtgaccgcg agcggcggca ccagcaccgc acgccgcagc gtatcggtgg tgataaacat
     3481 ctccgcctgc tcggcgagat ggatatcggg cggcacctgg tcgaacccga cctccaccag
     3541 ccgttcctga ttcacgggat cgctttggat ctcgatacgg gccacgtgtc ccggcagcgc
     3601 ccgtgacgga tcggagcgca gcacgatcgt cgcggcctga cccaccgcaa cacgaccggc
     3661 caggcgctca tcgacatagc caagcaccca gatcgtcgtt ggatcgacca gggtgaagac
     3721 cgactgcccg ggcaccggca tgctgccaag ctggaggttg cgtgacacga cccaggcatc
     3781 atagggcgcc cgcaacgtat cgttgtccgc ggcgaccgtg gcgtactcct cctgcgcctg
     3841 cgcggcggca agcgcggcct gcgccacggc gacctcgctc tgggcaacgg cgacattggc
     3901 acgcgccaca cgcatggccg cgtcgttggt ctgggcgacc tccaccgaca cgacgccgct
     3961 gcgcgccagg gcggtacgcc ggcgactgac ctgttcggca ttgaacaggc tcgcttcggc
     4021 gctggcgacg tcggccgtgg ccttgccgat attcgcctgc gcctgctgca cgccggcatg
     4081 ggccgaggcg cgttgcgcca ggacgtcgcg atcgtcgagt tgcgccagga cgctgccggc
     4141 cgccacgtga tcgccggcat ctgcatgcag ggcgaccagg gtaccggcga ccttgaaacc
     4201 gacattggat tggacccgcg cgcccaccgt gccaaggccg aacacttgcg ccggcacggt
     4261 gctggcggtg ccggcgacat ggacggtgat gggatgcagc caggcgcgcc agagctgcaa
     4321 accggccagg gcggcgaaca acaggacgag cccggccaac agcaggttgc gcccggaagg
     4381 gcgaggaagc cgccagcggg ggcggccccg gggaaccgcc tgctgcgggc tggttggcgc
     4441 gagactggcc ggctgcaacg gcatgacggg catcacagac cacgcttctt gccagagccg
     4501 gatcatgcgg cgtcaccggc ataccggcct tgacctgggt caatcgcgcg gccaccgctg
     4561 ccgcccgtgt cgcgcgtgtt gacgctcccc cgcgggatgg tgaaactggt ccccatgccg
     4621 ttgcaggtgc tcgatcccac caacgaatcc gccccgccgc tgggccagtc cgcgccgcgg
     4681 ctggcgtcgc tggccggcaa gacggttggg ttcatctcca acggcaagga ggggacgaag
     4741 gggttcttcg cgcatctcga tcgcatgctg cgggaagaat tcggcgttgc cgacgtggtc
     4801 atgcgggtga agtcgaatta cagcgccccg gccgatccgg acattgtgga cgagatcgcg
     4861 cgctggcacg cggtggtcac cggcatcggc gactgaggca gttgctcgtc gtgcagtctg
     4921 catgactcca tcaccgcaga gcgtcttggc atccccgcga tggcggtgat gaccacgcgc
     4981 ttcgtcagcg ccgcggaaat gatggcgcgg gtgctgggtt tcccggacta cgcgttcgcg
     5041 gtgattgcgc atccggtcag cagtgccggc gatgccgcgt tgcgcgacat ggccgccgcc
     5101 gccatccagc aggcgcggac gctgctgctg cgaggctgag gctgctactc ccccggcgct
     5161 ggcgcggggg ccggccgccg ttcgtgccgc cgcatgagca gcaacagcag cagcggcggg
     5221 atcaccacga agcacatcat gcggaaatcg tcgttgaagg cgatgatctg cgcctggtga
     5281 ttgatcagcc ggtccagcag agccgcgccg tgtcgcgttg ccggatccag caagcgggac
     5341 accgtgtcgt gcccctgcaa cgtgcgatcg aacggcgtga tctgcgccgc gatgccggca
     5401 tgcgatgtct gcatgttgcg caccagcatc agcgaggtga ccgacacgcc cacagcctga
     5461 cccaggttgc ggcacagcga ctgcagcgac gtggcatagc cccgcaggtg agacggcagc
     5521 gtggtgaagg cgaccacggt catcgggttg aacaccagcc cggtgccgaa gccctgcacc
     5581 atcagcgtca ccatcatcac atgctgggcc acgtcgggtg tccagaagct gatcgaatag
     5641 agcgccgatc ccatggtcag caggcccgtc gccatcagct tgcggtggtc gatccggtgc
     5701 gacagccggc tggccacctg catgccgatc atcaccccca acccgcgcgg cgccagcgac
     5761 catccggcgg tttccaccgg gtactgcgcc agggtttcca gatacggcgc catcagcgcg
     5821 gaactcgcca gcatcaccgt ggcggtgcag aacaccatcg
//
LOCUS       DASSZH010000263         7458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_892518, whole genome shotgun sequence.
ACCESSION   DASSZH010000263 DASSZH010000000
VERSION     DASSZH010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7458
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892518"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            328..795
                     /gene="tssJ"
                     /locus_tag="VFN42_05660"
     CDS             328..795
                     /gene="tssJ"
                     /locus_tag="VFN42_05660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HET8996136.1"
                     /translation="MGRWLSGAAAFALLAVLAGCSPPPPPPPTVVKLTLSATPDANPD
                     QAGQGAPVAIRVYQLSSTSAFDNAEFFPLFNNDTGTLKDTLVQRDQFILAPGKSKTVT
                     LTPKDQVKSLGFLAAYRDFQHATWRAEVEVPPHKTTTVTVTAGKTGITAKPGP"
     gene            798..2135
                     /gene="tssK"
                     /locus_tag="VFN42_05665"
     CDS             798..2135
                     /gene="tssK"
                     /locus_tag="VFN42_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HET8996137.1"
                     /translation="MGWENKVVWMEGLFLQPQHFQQQDRYVERLVRSSTAGLRPFAYG
                     LTQLELDSDLLALGKFALRAAAGILPDGTPFSIPGDVEHPQPIDVPEIARNGAVYLIL
                     PARQPGGVDAAPADRVETVARFTIGEHEATDTNAGFQNAAILPVGKLRLRYALEGEAR
                     EGFTGIGLARIAEVRPDKSVQLDEAYIPPLLLTSASPMLAGFVTQLQGLLHHRAEALA
                     GRVSETATRGAAEIADYLMLQLCNRYEPLLTHLAGTVNQLHPETLYRVCVSLAGELAT
                     FTETRKRPAEFPTYRHDDLQATFRPVIAALRQSLSAVLEQTAVPIPLQQRKFGIRVGP
                     IADRTLVASATWVLAVKAQMPAEALRRGFPNLVKIGPVEQIRELINVALPGVAVRALP
                     VAPRQLPYYAGTTYFELDRSSPYWAALARSGGIAIHVTGDVPGLEIECWAIRG"
     gene            2143..3489
                     /gene="tssL"
                     /locus_tag="VFN42_05670"
     CDS             2143..3489
                     /gene="tssL"
                     /locus_tag="VFN42_05670"
                     /inference="COORDINATES: protein motif:HMM:NF038228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, long
                     form"
                     /protein_id="HET8996138.1"
                     /translation="MSDDPFAEPSDVEATIIRPVPGGRSGPAAAPRPAPRPQPAATGT
                     VPTVGANPLLAAAAPLLAAAIRIGASRGRNPDPEQLRRGMVEAVREFEKRALATGLDT
                     RSLRAARYALCATIDDLVLSTPWGSTSTWAAQSLTSIFHNEVSGGERFFDILQDMEKD
                     LGRHSPVVELMYLCTSLGFEGRYRVMPRGIAALTELRDSVYRTIRQRRGDFERELSPR
                     WRGLETGYKPLVQRVPLWWLAVGTLCLACLIYLFFDFSLAAASDVAFSEMVGVPPHGT
                     ISVPRVAVVPPPPPPSPAVMAAASPAEPNFSQFLAPEIKQGLVKVFEDPQAVTVRLVN
                     RNMFASGSATLNHSYLPLLDRIGAELQKAKGDILINGYTDNQPIHTVQFPSNWQLSQA
                     RADAVASIVKAKLADPKRVRVVGKGDTDPIASNATPQGRELNRRTEIVLMKAAKPL"
     gene            3486..7130
                     /gene="tssM"
                     /locus_tag="VFN42_05675"
     CDS             3486..7130
                     /gene="tssM"
                     /locus_tag="VFN42_05675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HET8996139.1"
                     /translation="MTWLTRFLQKHWVEWLLAALVLSILVWFFGPLLGLGSLHPLTTI
                     FQRLLAITVIFVIWLIAVLLHELHEHKKEKELEEGVAEAAAEHDPAATASAEEVALLS
                     ERMKEALHQLKRAKLGGRSRRHLYQLPWYMFIGPPGAGKTTALVNCGLNFPLADEHGP
                     KAIQGVGGTRNCDWWFTDQAVLIDTAGRYTTQDSNTAVDSAAWFGFLKLLKKHRSRQP
                     LNGLLVAISLSDLALLPEQERLAHARAIRKRVRELADELGMRIPTYVLFTKADLVAGF
                     VEFFDSLGKEEREQVWGMTFALDNGNSEGGAVADFTQQFDQLLERLNDRMLERVHQEP
                     DLQRRRLIYGFPQQIASLRDIANEFLTEAFRPSRLEARPLLRGVYLTSGTQDGTPIDR
                     LLGAMAGQFGLARQAVSAFSGAGRSYFLARLIREVVFGEAGLVGRDPKLERRQRWRYI
                     GAYATCAVVLLALTTLWAVSWFGNRELIAADHQQIAKYNTEYAALTRRGPQDTDLPAV
                     VPALDTLREMHAGYGDRAEATPISLTYGLYQGVKLRSSAIDTYDRALNGLLLPRLLWQ
                     LEVQMQRHMNQPDFLYEALKVYLMLGREGPLDRKLIEEWAKADFAASFPGEENAAMRR
                     QLLEHVEALLQYPLAKVELNGPLVAQVRDILTREPLSQYIYSRIMHSEAVKELPVWSV
                     AEHAGPSGSRVFELRDGKPLNTGVPGIFTWEGYHQIFLPLLAPATKNASEASWVLGRP
                     EAENAAKTLEEMNQLHRALLGEYLDEYTRRWDDMLANIALKPFANMQQGLNELYLLSA
                     PASPLRDLLEAIDEETQLTHPAGGEKKGEKKNELAEKAQEFAKEAGHLSLTYEQNEIA
                     GILTAAYGVNASGKPNDPAIRVDQHFENLHVFVVGKPQQPPGLDAAIKQIQQVYEGML
                     GAANAPNQGAALLGMVGAGGAGGAGGSGGAAGAAQQLQQIAQSVPKPVAAMLQTVSQS
                     SSQVTASGASSELEGAWKSQVLPLCQEAFNRYPFVAGSAADVPIGDFVHLLGPDGLMQ
                     KFFDQYLKPFVNTTTQPWSWQSADHTKLGLSAGVLAEFQRADNIRQALFSNGGSQPMV
                     QFQLIPVSLDPGLAQVSLDIAGATLSYAHGPIEPMMMQWPGKGGNTLVRLTMTAANGG
                     PESVTQKDGTWSLLRLLDSGHLIPTGQPDKFHIVFSSPAGKAEFELNASSVRNPFNMS
                     LRAFRCPPKL"
     gene            7240..>7458
                     /locus_tag="VFN42_05680"
     CDS             7240..>7458
                     /locus_tag="VFN42_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996140.1"
                     /translation="MSESTTTYIDVSISPDPGFKLVFDSLDGTEELGRPFLYVLMMSS
                     EKAKGDLMALLGSSVTVSITKENKEKRHF"
BASE COUNT         1305 a         2685 c         2357 g         1111 t
ORIGIN      
        1 gatcgacgtt gcgggactgt atgccgatgc attggccgcg atgccggaga cgatcgacgg
       61 ccagcccccg ctgccggtac cgcaggattg ccccgtcaca ctggacgaac tgctgcgcga
      121 ctagacgcga agcggagccg cggcatgcgg ggcgattgcg cccgcgccca taacgccttg
      181 cgccccatgt cgccctttca atagaaaatt ccggacaccc ggtccgctcg tgtatagaag
      241 cccaccggca acgcgacacg acagggcgtg ccacacaccg atgcagcggg gccatacagg
      301 tcgccgcgtt gggggagacg gagcagcatg ggtcgatggc tttctggagc agcggcattc
      361 gcgctgctcg ccgttcttgc cggctgctcg cccccgccgc cgcccccgcc gaccgtggtc
      421 aagctcaccc tgagcgcgac cccggacgcc aatcccgatc aggccggaca aggcgccccg
      481 gtggccatcc gcgtctacca gctcagctcc accagcgcct tcgacaacgc cgagttcttc
      541 ccgctgttca acaacgacac cggcacgctg aaggacaccc tggtgcagcg tgaccagttc
      601 atcctggccc ccggcaagag caagaccgtc accctcacgc cgaaggacca ggtcaagtcg
      661 cttggcttcc tcgccgctta tcgcgatttc cagcacgcga catggcgcgc cgaggtcgag
      721 gtcccgccgc acaagaccac cacggtgacc gtcaccgccg gcaaaaccgg catcaccgcc
      781 aagcccggcc cctgaccatg ggctgggaga acaaggtcgt ctggatggag gggctgttcc
      841 tccagccgca acacttccag cagcaggacc gctacgtcga acgcctggtg cgcagcagca
      901 ccgccgggct gcggcccttc gcctatggcc tgacccagct ggaactcgat tccgacctgc
      961 tggccctggg caagttcgct ctgcgcgccg ccgccggcat cctgccggac ggcacgccct
     1021 tcagcattcc cggcgacgtg gaacacccgc agccgatcga cgtgccggaa atcgcccgca
     1081 acggcgcggt atacctgatc ctgcccgctc gccagcccgg cggcgtggat gcggccccgg
     1141 ccgaccgggt ggaaaccgtg gcgcgcttca ccatcggcga gcacgaggcc accgacacca
     1201 atgccggctt ccagaacgcc gccatcctgc ctgtgggcaa gctgcgcctg cgctacgcgc
     1261 tggaaggcga ggcgcgcgaa ggcttcaccg gcatcggcct ggcccgcatc gccgaagtgc
     1321 gtcccgacaa aagcgtgcag ctcgacgagg cctatatccc gccgctgctg ctgaccagcg
     1381 cctcccccat gctggcaggc ttcgtcaccc agcttcaggg cctgctgcat caccgcgccg
     1441 aggcgctggc cggccgcgtg tcggaaaccg ccacccgcgg cgcggcggag atcgccgact
     1501 acctgatgct gcagttgtgc aatcgctacg agcccttgct gacccatctg gccggcacgg
     1561 tgaaccagtt gcacccggaa accctctacc gggtctgcgt cagcctggcc ggcgaactcg
     1621 ccacctttac cgagacccgc aagcgcccgg cggaattccc cacctaccgg catgacgacc
     1681 tgcaggcgac cttccgcccg gtcatcgccg cgctgcgcca gtcgctgtcc gccgtgctgg
     1741 agcagaccgc ggtgccgatc ccgctgcagc agcgcaagtt cggcatccgc gtcggcccga
     1801 tcgccgaccg cacgctggtc gccagcgcca cctgggtgct cgcggtcaag gcgcagatgc
     1861 cggccgaggc gctgcgccgc ggcttcccca acctggtgaa gatcggcccg gtcgagcaga
     1921 tccgcgaact gatcaacgtc gccctgcccg gcgtcgcggt gcgcgccctg ccggtcgccc
     1981 cccgccagct gccgtactac gccgggacca cgtatttcga actcgaccgc agcagcccgt
     2041 actgggcggc actcgcccgt tcgggcggca ttgcgatcca cgtcacaggc gacgtgccgg
     2101 gactggaaat agaatgctgg gcgatcagag ggtgagcagc cgatgagtga tgatcccttc
     2161 gccgaaccat ccgacgtcga agccaccatc atccgcccgg tacccggcgg acgcagcggc
     2221 cccgcggcag caccccggcc cgccccccgc ccgcaaccgg ccgccaccgg cacggtaccc
     2281 acggtcggcg ccaatccgct gctcgccgcc gccgccccgc tgctcgccgc cgccatacgc
     2341 atcggtgcca gccgcggccg caatcccgac cccgaacagc tacgccgcgg catggtcgag
     2401 gcggtgcgcg aattcgagaa acgtgccttg gccaccggcc tggacacccg cagcctgcgc
     2461 gccgcgcgct atgcgctgtg cgccaccatc gacgacctgg tgctcagcac accctggggc
     2521 agcaccagca cctgggccgc acagagcctg accagcatct tccacaacga ggtcagcggc
     2581 ggcgaacgct tcttcgacat cctgcaggac atggagaagg acctcggccg ccacagcccg
     2641 gtggtcgagc tcatgtatct ctgcacctcg ctcggcttcg aggggcgcta ccgcgtcatg
     2701 ccgcgcggca tcgccgcact gaccgagctg cgcgattccg tctatcgcac catccgccag
     2761 cgccgcggcg atttcgagcg tgagctctcg ccgcgctggc gcggcctgga aaccggctac
     2821 aagccgctgg tgcagcgcgt gcccctatgg tggctcgctg tcggcacgct gtgcctggcc
     2881 tgcctgatct acctgttctt cgacttttcc ctggccgccg cgtccgacgt ggcgttcagc
     2941 gaaatggtcg gcgtgccgcc gcacggcacc atcagcgtgc cgcgcgtcgc cgtggtcccg
     3001 ccgcctcccc cgccctcgcc ggcggtgatg gccgccgcca gccccgccga accgaatttc
     3061 agccagttcc tcgccccgga gatcaagcag gggctggtca aggtgttcga ggatccgcag
     3121 gcggtcaccg tgcggctggt caaccgcaac atgttcgcct caggctccgc cacgctcaat
     3181 cacagctacc tgccgctgct ggatcggatc ggcgccgaac tgcagaaagc caaaggcgac
     3241 atcctgatca acggctacac cgacaaccag ccgatccata ccgtgcagtt cccgtccaac
     3301 tggcaattgt cccaggcccg cgccgacgcc gtggccagca tcgtcaaggc gaaactggcg
     3361 gatccgaagc gtgtgcgcgt cgtcggcaag ggcgacaccg acccgatcgc cagcaacgcc
     3421 acgccacaag gacgcgaact caaccggcgc accgagatcg tgctgatgaa ggcggcgaaa
     3481 cccctatgac ctggctgacc cgttttctgc agaagcattg ggtcgagtgg ctgctcgccg
     3541 cgctcgtgct ttccatcctc gtctggttct tcggcccgct gctcggcctc ggctcgctgc
     3601 atccgctcac caccatcttc cagcgcctgc tcgccatcac ggtcatcttc gtgatctggc
     3661 tgatcgccgt gctgctgcac gagctgcacg agcacaagaa ggaaaaggag ctggaggaag
     3721 gcgtcgcgga agcggccgcg gagcatgacc cggccgccac cgcctcagcc gaggaagtcg
     3781 cgctgctgag cgaacgcatg aaggaggcgc tgcaccagct caagcgcgcc aagctcggcg
     3841 gccgatcccg gcgccatctg taccaactgc cctggtacat gttcatcggc ccgcccggcg
     3901 ccggcaaaac cactgcgctg gtcaattgcg gcctgaactt ccccctggcc gacgagcacg
     3961 gacccaaggc catccagggc gtcggcggca cccgcaactg cgactggtgg ttcaccgacc
     4021 aggcggtgct gatcgatacc gccggccgct acaccaccca ggattccaac accgcggtgg
     4081 attccgccgc ctggttcggc ttcctcaaac tgctcaagaa gcaccgcagc cggcagccgc
     4141 tgaatggcct gctggtggcg atcagcctgt ccgacctcgc tctgctgccg gagcaggaac
     4201 ggctggcgca tgcccgcgcc attcgcaagc gggtgcgcga actggccgac gaactcggca
     4261 tgcgcatccc gacttatgtg ctgttcacca aggccgacct ggtcgccggc ttcgtcgagt
     4321 tcttcgacag cctgggcaag gaggaacgcg agcaggtctg gggcatgacc ttcgcgctgg
     4381 acaacggcaa cagcgaaggc ggcgccgtcg ccgacttcac ccagcaattc gaccagctgc
     4441 tggaacggct gaacgatcgc atgctggaac gcgtgcacca ggaacccgac ctgcagcgcc
     4501 gccggctgat ctacggcttc ccgcagcaga tcgcctcgct gcgcgacatc gccaacgagt
     4561 tcctgaccga ggcgttccgc cccagccggc tggaagcccg cccgctgctg cgcggcgtct
     4621 atctcacctc cggcacccag gacggcacgc cgatcgaccg gctgctgggc gcgatggccg
     4681 gccagttcgg cctggcgcgg caggcggtca gcgccttcag cggcgccggc cgcagctatt
     4741 tccttgcccg gctgatccgc gaagtggtgt tcggcgaggc ggggctggtc ggccgcgatc
     4801 ccaagctcga gcggcggcag cgctggcgct atatcggcgc ctacgccacc tgcgcggtgg
     4861 tgctgctcgc cctgaccacg ctgtgggccg tcagttggtt cggcaaccgg gagctgatcg
     4921 ccgccgatca ccagcagatc gccaaataca acacggaata tgccgccctg acccggcgcg
     4981 ggccgcagga caccgacctg ccggccgtcg tgcccgcgct cgacaccttg cgcgagatgc
     5041 atgccggcta cggcgaccgg gccgaggcaa ccccgatctc cctcacctat ggcctctacc
     5101 agggggtgaa gctccgcagc agcgcaatcg acacctatga ccgcgcgctg aacgggctgc
     5161 tgctgccgcg gctgctatgg cagctcgaag tgcagatgca gcggcacatg aaccagccgg
     5221 atttcctgta tgaggcgctg aaggtctacc tgatgctcgg gcgcgagggg ccgctcgacc
     5281 gcaagctgat cgaggaatgg gccaaggccg atttcgccgc gagcttcccc ggcgaggaga
     5341 acgccgccat gcgccggcaa ttgctggagc atgtggaggc gctgctgcaa tacccgctgg
     5401 caaaggtcga actgaacggg ccgctggtcg cccaggtgcg cgatatcctc acccgcgaac
     5461 cgctgtcgca gtacatctac agccgcatca tgcacagcga ggcggtgaag gagctgccgg
     5521 tctggagcgt ggcggagcat gccggcccgt ccggcagccg cgtgttcgag ctgcgcgacg
     5581 gcaagccgct gaacaccggt gttccgggca tcttcacctg ggagggctat caccagatct
     5641 tcctgccgct gctggcgccg gcaaccaaga atgcctcgga agcctcctgg gtactcggcc
     5701 ggccggaagc ggaaaacgcc gccaagacgc tggaggagat gaaccagctg caccgcgcgc
     5761 tgctcggcga atatctcgac gaatacaccc ggcgctggga cgacatgctg gccaacatcg
     5821 ccctgaagcc cttcgccaac atgcagcagg gcctgaacga gctgtacctg ctttcggcgc
     5881 ccgcctcgcc gctgcgcgac ctgctggagg cgatcgacga ggaaacccag ctcacccatc
     5941 ccgccggcgg cgaaaagaag ggggagaaaa agaacgagct ggccgaaaaa gcccaggagt
     6001 tcgccaagga ggcgggccat ctgagcctga cctacgagca gaacgagatc gccggcatcc
     6061 tgaccgccgc ctatggcgtg aacgccagcg gcaagccgaa cgatccggcc atccgggtgg
     6121 accagcattt cgaaaacctg cacgtgttcg tggtcggcaa gccgcagcag ccgccgggcc
     6181 tggatgccgc tatcaagcag atccagcagg tctatgaagg catgctgggc gcggccaatg
     6241 cgccgaacca gggcgccgcg ctgctgggca tggtcggcgc cggtggggcc ggcggcgcgg
     6301 gtggcagcgg cggggccgcc ggagccgcgc agcaactgca gcagatcgcg cagagcgtcc
     6361 ccaagccggt cgccgccatg ctgcagacgg tgtcgcaatc cagctcccag gtgactgcca
     6421 gcggcgccag ctcggagctg gagggtgcct ggaaatcgca agtgctgccg ctctgccagg
     6481 aagcgttcaa ccgctatccc ttcgtcgccg gcagtgcggc ggacgtgccg atcggcgact
     6541 tcgtgcatct gctcgggccg gacgggctga tgcagaaatt cttcgaccag tacctgaagc
     6601 ccttcgtgaa caccacgacg cagccctgga gctggcaatc ggcggaccac accaagctgg
     6661 gcctctcggc cggcgtgctg gccgaattcc agcgcgccga caacatccgc caagcgctgt
     6721 tctccaacgg cggttcgcag ccgatggtgc agttccagct gataccggta tcgctcgatc
     6781 cgggcctggc gcaggtcagc ctggatatcg ccggcgcgac gctgagctat gcgcacggcc
     6841 cgatcgagcc gatgatgatg cagtggccgg gcaagggcgg aaacacgctg gtgcggctga
     6901 ccatgaccgc agcgaatggc gggccggaat cggtcaccca gaaagacggc acctggtcgc
     6961 tgctgcgcct gctggattcc gggcacctga tcccgaccgg gcagccggac aagttccaca
     7021 tcgtgttcag cagccccgcc ggcaaggcgg agttcgagct gaacgcgtcc agcgtgcgca
     7081 atcccttcaa catgtcgctg cgggctttcc gctgcccacc gaaactctga aacgttgcgg
     7141 tcagcacagg agctggacaa tggtgccgga gagcctttct gacctgacgt gagtgtcgcc
     7201 ggggcggttc ccggacgcgg caatgacccg aggcaaggta tgtcggagtc gaccaccacc
     7261 tatatcgacg tcagcatttc tccggatccc ggattcaagc tggtgttcga ttcactcgac
     7321 ggcaccgagg aactgggccg gcccttcctg tacgtcctga tgatgtcgag cgaaaaggcc
     7381 aaaggcgacc tgatggcgct gctcggcagc tcggtgaccg tctccatcac caaggagaac
     7441 aaggagaagc gccacttc
//
LOCUS       DASSZH010000264         4123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1352496, whole genome shotgun sequence.
ACCESSION   DASSZH010000264 DASSZH010000000
VERSION     DASSZH010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352496"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..1052
                     /gene="moaA"
                     /locus_tag="VFN42_05685"
     CDS             66..1052
                     /gene="moaA"
                     /locus_tag="VFN42_05685"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HET8996141.1"
                     /translation="MIDPFGRKITYLRVSVTDRCDLRCVYCMAEDMHFLPKREVLTLE
                     ELDRICAAFIRLGTNKLRITGGEPLVRRDVMTLFRQLGARIGSGLNELTLTTNGTQLA
                     KHAEGLFAAGVRRVNVSLDTLDPVKFSDITRRGNIEPVLDGIFAAKAAGLAVKINAVA
                     LKGVNEDEYDAMLAWCGEHGFDLCLIETMPLGEISEDRTEQYLPLSIVRGRLRKRWTL
                     EDTDYRTGGPARYFTIRETGRRVGFITPMTHNFCESCNRVRLTCTGTLFMCLGQEDSA
                     DLRSVLRAGASDEQLDDAIRAAIARKPKGHDFIIDRRHQQPAVGRHMSTTGG"
     gene            complement(1371..2366)
                     /locus_tag="VFN42_05690"
     CDS             complement(1371..2366)
                     /locus_tag="VFN42_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET8996142.1"
                     /translation="MPDRADQSHGHAHGHDHDHGRGHDDGHGHHHGIGGHHHAPASFG
                     RAFAVGVTLNLAYVLAEAFWGFSAHSVALLADAGHNLGDVAGLLGAWLAAWLAQRGPS
                     EQYTYGLRRSSILAALANALILLVVTGAIAWEAIQRLITPEPTGGFVIMVVSLGGVLV
                     NGITALMFMSGRKHDLNIKGAFMHMAADALLTLGVAVAGAVIMLTGWQRLDPAVSLIL
                     SVVIILGTWSLLRDSVSLSMDSVPPGIDQQEVGRYLRALPGITEVHDLHIWGMSTTET
                     ALTAHLVRADGNSDGDLLHRVTHEIRERFNIGHATLQFETQEMASRCELRPDHVV"
     gene            2453..2770
                     /locus_tag="VFN42_05695"
     CDS             2453..2770
                     /locus_tag="VFN42_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET8996143.1"
                     /translation="MAPETSTAPAPLAELAEMFRLMGDPSRLSIILACLQAPLAVAEV
                     AERTGLSANLVSHHLRLLRTGRILRGERRGKQVFYTAADDHIRRTIGDMVAHVGEPRS
                     GGA"
     gene            complement(2814..3149)
                     /locus_tag="VFN42_05700"
     CDS             complement(2814..3149)
                     /locus_tag="VFN42_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996144.1"
                     /translation="MMALAIYCLAALGEISGCFAFWAWLRLQHSVLWVIPGMLSLAAF
                     ALLLTRIDVHLAGRAFAAAAYGGVTIAASLLWLWVVEGQRPDGWDMLGAALALLGAMA
                     IVGTHALRR"
     gene            complement(3146..3561)
                     /locus_tag="VFN42_05705"
                     /pseudo
     CDS             complement(3146..3561)
                     /locus_tag="VFN42_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639603.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            3638..>4123
                     /locus_tag="VFN42_05710"
     CDS             3638..>4123
                     /locus_tag="VFN42_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HET8996145.1"
                     /translation="MPDATATEAARPGAALLRYRVSGMDCPSCAGKIETALQRLHGTG
                     DIRINFHTQMLALRLDEAATSRDRIEASLRQLGFGIAPADVSAFAADPVEAGDRQAVP
                     AWLAWLRTLKARLTLAIGLLLIAGFVVARLQPGIGHWAYLPAALLGLAFFGRRAVALA
                     RA"
BASE COUNT          715 a         1428 c         1341 g          639 t
ORIGIN      
        1 caagtgaatc cccagcgtcg cccggccctg gtcgcagggc gggaggagga gccagagttc
       61 gtaccatgat cgacccgttc ggccgcaaga tcacctatct ccgcgtttcg gtcaccgacc
      121 gttgcgatct ccgctgcgtc tactgcatgg ccgaggacat gcacttcctg cccaagcgcg
      181 aagtgctgac cctggaggaa ctcgaccgca tctgtgccgc cttcatccgc ctgggcacca
      241 acaagctgcg catcaccggc ggcgagccgc tggtccgccg cgacgtcatg accctgttcc
      301 gccagctcgg cgcccgcatc ggcagtggcc tgaacgagct gacgctgacc accaacggca
      361 cgcaactggc gaagcacgcg gagggactgt tcgcggccgg cgtgcgccgg gtgaacgtgt
      421 cgctggacac gctcgacccg gtgaagttct ccgacatcac ccgccgcggc aatatcgagc
      481 cagtgctgga cggcatcttc gccgccaagg ccgcgggact tgccgtcaag atcaacgcag
      541 tggcgctgaa gggcgtgaac gaggacgagt acgacgccat gctggcctgg tgcggcgagc
      601 acggtttcga cctgtgcctg atcgagacca tgccgctggg cgaaatctcc gaggatcgca
      661 ccgagcaata cctgccgctg tccatcgtcc gcggccggct gcgcaagcgc tggacgctgg
      721 aggacacgga ttaccgcacc ggcggcccgg cacgctattt caccatccgg gaaaccgggc
      781 ggcgagtcgg attcatcacg ccgatgaccc acaatttctg cgaaagctgc aaccgcgtgc
      841 ggctaacctg caccggcacg ctgttcatgt gcctggggca ggaggattcc gccgatttgc
      901 gctcggtgct gcgcgccggc gcgagcgacg agcaattgga cgacgccatc cgcgccgcga
      961 tcgcgcgcaa gcccaaaggc catgacttca tcatcgaccg ccgccatcag caacccgcgg
     1021 tgggtcggca catgagcacg accggcggct gatggctctg ctgcgaagag tgtaacgagt
     1081 cagaggttct ggcctgcggc gtggccgctt gtcgggcagg actgcgtcca gacagcaggc
     1141 acaggtcgca cgcggacgag gttgagctgc cgcttgcgga tgcgttgccg tcgtttgatc
     1201 tccgctgggg tgctcccgcg gtcgcgaacc ttggccggcc gcaaggtgtt gcacggcgcc
     1261 gaccagtttt ggcgagggtt cccgccagct cgtccaggcg gcgccgaggc tggccacgat
     1321 caccgggatg aaagcgtaca gccacatcac gccttccgtg ccggccgcat tcaaacgaca
     1381 tgatccggcc gcagctcgca gcggctcgcc atctcctgcg tctcgaattg cagggtggcg
     1441 tggccgatgt tgaaccgttc gcggatttcg tgcgtgaccc gatgcagcag gtcgccgtcg
     1501 ctgttgccat ccgcgcggac gagatgcgcg gtcagcgcgg tctcggtggt gctcatgccc
     1561 cagatgtgca ggtcgtgcac ttcggtgatg ccgggcagcg cacgcaggta gcgcccgacc
     1621 tcctgctggt cgataccggg tggcacgctg tccatcgaca ggctcaccga atcgcgcagc
     1681 agcgaccatg tgccgaggat gatcaccacg ctgagaatca ggctgacagc cgggtccaac
     1741 cgctgccatc cggtcaacat gatgacggcg ccggcgacgg cgaccccgag ggtaagcagg
     1801 gcgtcggccg ccatgtgcat gaaggctccc ttgatgttca ggtcgtgctt gcgaccggac
     1861 atgaacatca gcgcggtgat gccattgacc agcacaccac caagcgacac caccatgatg
     1921 acgaagccgc cggtgggttc cggggtgatc agccgctgga tcgcctccca ggcgatggcg
     1981 ccggtgacca ccagcaggat cagcgcgttc gccagcgcgg cgaggatcga ggagcggcgc
     2041 aggccatagg tgtattgctc actcgggcca cgctgcgcca gccaagccgc cagccaggcg
     2101 cccagcagtc ccgccacgtc acccaggtta tgcccggcat cggccagcag ggcgaccgaa
     2161 tgcgccgaga agccccagaa agcctcggcc aacacgtaag ccagattcag cgttacgccg
     2221 acagcgaagg ctcggccgaa gctggccggc gcgtgatggt ggccaccgat cccatgatga
     2281 tgtccgtgtc catcatcatg cccgcggccg tgatcatgat cgtgaccatg ggcatggccg
     2341 tggctttggt ccgcgcggtc gggcatgcgg tcactccgaa aggcttgaac aggccaagcg
     2401 ttagattata acatcacagg aacacctatt cctgcattcc ccagatttgg ccatggcccc
     2461 cgaaacgtca accgcacccg ccccgctcgc cgaactggcg gaaatgttcc ggctgatggg
     2521 tgaccccagc cggctgtcga tcatcctggc ctgcctgcag gcgcccctcg ccgtcgccga
     2581 agtggcggaa cggaccgggc tatcggcaaa tctggtcagc catcacctgc gcttgctccg
     2641 gaccggccgc atcctgcgcg gcgagcggcg gggaaagcag gtgttctaca ccgccgccga
     2701 cgatcacatc cgccgaacca tcggcgacat ggtcgcgcat gtcggcgagc cgcgcagcgg
     2761 tggggcatag gaatcgccgg gggccgcgct tatccggcga tcagcacccc cggtcaccgc
     2821 ctcagcgcat gtgtcccaac gatggccatc gcccccagca gagccagcgc ggcacccagc
     2881 atgtcccacc cgtccggccg ctgcccttcc accacccaga gccagagcag gctcgcggcg
     2941 atagtgacgc cgccataggc ggcagcggca aaggcccgcc cggcgaggtg cacgtcgatc
     3001 ctggtcagca gcaaggcgaa ggccgcgagc gacagcatgc ccgggatcac ccacagcacg
     3061 gaatgctgca aacgcagcca tgcccagaag gcgaagcagc cggagatttc tcccagcgcc
     3121 gccaggcagt agatcgccag tgccatcacg ccgccatggc ttgtgctggc gagagcgcct
     3181 ggaggatccg gcactgcgcg atgctcccct gactgcagga tgcgatcagc tccgcaactc
     3241 ccgccgcagc gcctccagat cggcgagctt gcgctccacc acctgcaggt gctcgcgcgc
     3301 gatgccgtcc acctcggcgc aggattgctc ccgctgatcg gcaagccgga gcagggtacg
     3361 cacctggtcc agcgagaatc ccagctcccg cgcccggcgg atgaagctga gccgcgccag
     3421 atgcgccggt ccgtagctgc ggtagttgcc ggcggtacgt tccggtggcg gtagcagccc
     3481 gacccgctcg tacaagcgca ccgtttccac cttgatgcca gtcgccttgg cgacggcacc
     3541 gatactcaag gactccgcca tggcttgacc ctgtagtgac tacaggcccc atctgggtcc
     3601 gcatccgctc caggcgcaac ccaaacggag gtcacgcatg ccagacgcga cggcaaccga
     3661 ggcggccagg ccaggcgcgg cgctgctgcg ttaccgtgtc tccggcatgg actgtcccag
     3721 ctgcgccggc aaaatcgaga cggcgctgca gcggctgcac ggcaccggcg acatccggat
     3781 caatttccac acccagatgc tagcgctgcg cctggacgag gccgccacgt cccgcgatcg
     3841 gatcgaggcg agcctccgcc agctcggctt cggcatcgcg ccggccgatg tgagcgcgtt
     3901 cgcggccgat cccgtcgaag ccggagatcg gcaggcggtt ccggcatggc tggcgtggct
     3961 gcggacgctg aaggctcggc tgacgctggc catcgggctg ctgctgatcg ccggtttcgt
     4021 cgtcgcgcgg cttcagccgg gtatcggcca ctgggcttac ctgccagccg cgctgctcgg
     4081 cctggcgttc ttcgggcgcc gagccgttgc gctggcgcgt gcc
//
LOCUS       DASSZH010000265         4758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_653991, whole genome shotgun sequence.
ACCESSION   DASSZH010000265 DASSZH010000000
VERSION     DASSZH010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653991"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VFN42_05715"
     CDS             <1..988
                     /locus_tag="VFN42_05715"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET8996146.1"
                     /translation="RSLTLNLKAPEGVQILHRLVQQADVVVENYRPDVKFRLGVDYES
                     LRKLNRRLVYASISGFGQDGPYRERPGFDQIAQGLGGLMSITGLPGQGPVRVGIPVAD
                     LTAGIFAAMGILIALLEREQSGEGQWVQSNLLAAQIAMLDFQAARWTIGHEVPGQAGN
                     DHPTSIPTGVFPTSDGHINIAAAGEEIYRRCCKALDAPQLADNPDFATGALRSKNRAR
                     LNESIAAITRTRTSAEWVERLNDAGVPAGPIYKMNEVFADPQVQHLHITRKVPHKVLG
                     EVEVIGQAIELSRTPWSVRSATPELGEHTDAILRELGYGSAEIESLRGKKVV"
     gene            1007..1633
                     /locus_tag="VFN42_05720"
     CDS             1007..1633
                     /locus_tag="VFN42_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET8996147.1"
                     /translation="MDAMPDHLIRDEATLSALYGEPPPGAIAKEVDHIHPHYRAMIAA
                     SPFVVLATSGPGGLDVSPRGDPAGFVCVEDDKTLLIPDRRGNNRADSLRNIVHDPRVA
                     LLFLIPGVGETLRVNGRAAISVSPALLERFVFRGALPRSVIVVSVETVYFQCPKALVR
                     SELWNPEKQISRKALPSTGTILADITAGRVGGEQYDREYPERLRKTLY"
     gene            complement(1778..2638)
                     /locus_tag="VFN42_05725"
     CDS             complement(1778..2638)
                     /locus_tag="VFN42_05725"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8996148.1"
                     /translation="MSTTPHEFTTEDREYLRHGDRVLKLRHFRPRGDGPFPAVIEVHG
                     GAWCKGDLNECQTYAEAFARAGLVVLALDFRHAGDGYPTSSIDINYATRWAKAHAAEL
                     GTHAGMIGIAGQSSGGHLAMLAAMRPADPRYTAVALPAGSPTVDASVGAVAMLWPVIN
                     PLSRYNHARRARDSANPPAWVGDIPERHDLYWKTTAAMADGNPMLALERGESVRLPPA
                     LWLQGRPDPVHDYRDPDAPIPGNEPERFVANYRKAGGEIALLVTEQAARASAETLGQV
                     AAFFHEHLRV"
     gene            complement(2926..4260)
                     /gene="purB"
                     /locus_tag="VFN42_05730"
     CDS             complement(2926..4260)
                     /gene="purB"
                     /locus_tag="VFN42_05730"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET8996149.1"
                     /translation="MVPRYTRPEMDAIWAPANRFRIWFEIEALAAEGMARIGTIPESA
                     ARTIRERGGAAAAQISQADIDRIDAIERETRHDVIAFLTWLAESVGPDSRFVHQGMTS
                     SDVLDTCLSVQLTQAADLLLADLDAVLDALKARAIEHKHTVTIGRSHGIHAEPTTFGL
                     KLAGHYAEFARNRARLVAARAEVAVAAISGPVGTYAQVDPRVEEYVAERLGLAVEPVS
                     TQVIPRDRHAAFFCTLGVIASGIERLATEVRHLQRSEVREAEEFFHPGQKGSSAMPHK
                     RNPVLSENLTGLARVVRGAVVPALENVALWHERDISHSSVERVIAPDATLALDFALVR
                     LAGMMQRLVVYPQRMAENLASLGGVVASGDVMLALAKSGIAREDAYRIVQRNAMATWT
                     KLGTPEGRSFRDNLLADPEVAGRVPVAELDAAMDPAAHVRAVDQVFARVFGA"
     gene            4356..>4758
                     /locus_tag="VFN42_05735"
     CDS             4356..>4758
                     /locus_tag="VFN42_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010501731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HET8996150.1"
                     /translation="MDISLLSQDLSALAQVVLIDVTLAGDNAVAVGMAVAGLPPRQRR
                     PAVMLGVAGATVVRVALGAVALELLAVIGLLLAGGLLLLWVCWKMFRELRHSAAARTS
                     LDRPHKTLGQAMLQIVLADLSMSLDNVLAVAG"
BASE COUNT          756 a         1635 c         1607 g          760 t
ORIGIN      
        1 gcgcagcctg acattgaacc tgaaggcacc ggagggcgtg cagatcctgc atcgcctggt
       61 gcagcaggcg gacgtggtgg tggagaacta ccggcctgac gtgaaattcc gcctgggcgt
      121 cgattacgag tcactgcgca agttgaaccg gcgtctcgtc tatgcctcga tttccggatt
      181 tggccaggac gggccgtacc gcgaacggcc gggcttcgac cagatcgccc agggcctggg
      241 cgggctgatg tcgatcaccg gcctgcccgg acaggggccc gtgcgtgtcg gcatccctgt
      301 cgccgatctc accgccggca tcttcgccgc catgggcatc ctgatcgcgc tgctggagcg
      361 cgagcaatcg ggcgagggcc aatgggtgca gtccaacctg ctcgcggcgc agatcgccat
      421 gctagatttc caggcggcgc gctggacgat cggccatgag gtgcccgggc aggccggcaa
      481 cgatcatccg accagcattc ccaccggcgt gtttccgacc agcgacggac acatcaacat
      541 cgccgccgcg ggagaggaga tctatcgccg ctgctgcaag gcgctggacg caccgcagct
      601 tgcggacaac ccggacttcg caaccggagc attacgatcc aagaaccgtg cacgcctgaa
      661 tgagtccatt gccgccatta cccgcacgcg gacctcggcg gaatgggtcg agcggctgaa
      721 cgatgcaggc gtccccgcgg ggccgatcta caagatgaac gaggtgttcg ccgatccgca
      781 ggtgcagcac ctgcacatca cgcgcaaggt gccgcacaag gtcctcggcg aggtggaggt
      841 gatcggccag gcgatcgagc tcagccgtac gccgtggagc gtacgcagcg cgacgccgga
      901 actgggggag cacaccgacg ccatcctgcg cgaactcggc tacggctcgg ccgagatcga
      961 gtcgctgcgc ggcaagaagg tcgtctagca ccaaggggcc agtgccatgg atgcgatgcc
     1021 cgatcacctg atccgcgacg aggcgacatt gtccgccttg tatggcgagc cgccgcctgg
     1081 cgcgatcgcc aaggaggtgg accacatcca cccgcattat cgcgcgatga tcgccgcgtc
     1141 gcctttcgtg gtgctggcca ccagcggtcc gggagggctg gacgtatcgc cgcgcggcga
     1201 tcccgccggg ttcgtctgcg tggaagacga caagacgctg ctgatccccg accggcgcgg
     1261 caacaaccgc gccgatagcc tgcgcaacat cgtgcacgat ccgcgcgtgg cactgctgtt
     1321 cctgatcccc ggcgtcggcg agacgctgcg ggtgaacggg cgcgcggcaa tctcggtttc
     1381 acccgccctg ctcgaacgct tcgtgttccg gggcgcgttg ccgcggtcgg tgatcgtcgt
     1441 gtctgttgag acggtttact tccagtgccc gaaggcgctg gtgcgctcgg aactgtggaa
     1501 tccggaaaag cagatttcgc gcaaagcgct gcccagcacc gggacgatcc tggcggacat
     1561 caccgcgggc cgggttggcg gcgagcagta cgaccgggag tatccggagc ggctgcgcaa
     1621 gacgctgtac tgaagccgcg aatgcaaaag aactcccccc tccccttgcg ggaaggggtt
     1681 ggggggaggg ggcctgcaag tgtcccccgg acaaccacac cgacccttcc cccagcccct
     1741 cccgcaagga gagggggagt tgttgaccca gattgcccta cacccgcaaa tgctcgtgga
     1801 aaaacgcggc gacctggccc agcgtctcgg cactcgcacg cgccgcctgc tcggtcacca
     1861 gaagcgcgat ctcaccgccc gccttgcgat agttggcgac gaaacgttca ggctcgttgc
     1921 cggggatcgg cgcatccggg tcgcgataat cgtgcaccgg atcggggcgg ccctgcagcc
     1981 acagcgctgg cggcaagcgc acgctctcgc cgcgttccag cgccagcatc gggttgccgt
     2041 cggccatggc ggcggtggtc ttccagtaca gatcgtgccg ttccgggata tcgccgaccc
     2101 aggccggcgg gttcgcggaa tcccgcgccc gccgagcatg gttgtagcgc gacagcgggt
     2161 tgatcaccgg ccacagcatg gcgaccgccc ccacgctggc atccaccgtg ggagagccgg
     2221 cgggcagcgc caccgcggtg tagcgcggat ctgccgggcg catcgccgcc agcatggcga
     2281 gatggccgcc gctggactgg ccggcgatgc cgatcatgcc ggcatgcgtg cccaactctg
     2341 ccgcatgcgc cttggcccag cgcgtggcat agttgatgtc gatgctggag gtcggatagc
     2401 cgtcgccggc gtggcggaaa tccagcgcca gcaccaccag cccggcgcgg gcgaaagctt
     2461 ccgcataggt ctggcactcg ttcaggtcgc ccttgcacca ggcgccgccg tgcacctcga
     2521 tcaccgcggg gaacggcccg tcgccgcgcg gacggaagtg ccgcagcttc agcaccctgt
     2581 cgccgtggcg caggtattcc cggtcctcgg tggtgaattc gtgcggtgtt gtggacatcg
     2641 gctgtcctcc ctggggtcgc atcttgacca gggagtgtag ccggttggcc ggccagcgaa
     2701 aggggcgggc ctggccgcca cagaagaatt ggaaccacag atgaacgtgg atgaacgcgg
     2761 atgctatgca gcgggccgta gcggactcgc atgcgtccgt tcagtcgggg aatgaagcat
     2821 gtttgcctgc agcgggaatt cgcacgactg atcagacccc accgcaccat ccgcgttcat
     2881 ctgcgttcat ctgcggtccc atacttcttc tacttgcttt tgagcctagg ccccgaacac
     2941 gcgggcgaat acctggtcca ctgcccggac atgcgccgcc gggtccatcg cggcgtcgag
     3001 ttccgccacc ggcacgcgcc cggctacctc cggatcggcc agcaggttgt cgcggaagct
     3061 gcgcccctcc ggcgtgccaa gcttcgtcca ggtcgccatc gcgttgcgct gcacgatgcg
     3121 ataggcgtcc tcccgcgcga tgccggattt cgccagcgcc agcatcacgt cgccgctggc
     3181 caccacgccg ccgaggctcg ccaggttctc tgccatgcgc tgcggataaa ccaccagccg
     3241 ctgcatcatc ccggccagcc gcaccagcgc gaagtccagc gcaagggtgg cgtccggcgc
     3301 gatcacccgc tccaccgagg aatgggaaat gtcgcgctcg tgccacagcg ccacattttc
     3361 cagcgccggc accaccgcgc cgcgcaccac gcgcgccagg ccggtgaggt tctcgctcag
     3421 caccgggttg cgcttgtgcg gcatggcgct ggagcccttc tggccgggat ggaagaactc
     3481 ctccgcctcc cgcacttcgc tgcgctgcag gtgacgcact tccgtggcca gccgctcgat
     3541 gccagaggca atcacgccca gcgtgcagaa gaacgcggcg tggcggtcgc gcgggatcac
     3601 ctgggtggac accggttcca cggccaggcc cagtctctcg gcgacgtatt cctccacccg
     3661 cggatcgacc tgggcatagg tgccgaccgg gccgctgatc gccgccaccg cgacctcggc
     3721 gcgcgccgcc accagccgcg cgcggttgcg ggcgaactcc gcgtaatgcc cggcgagctt
     3781 caggccgaag gtggtgggtt cggcgtggat gccgtggctg cggccgatcg tgacggtgtg
     3841 cttgtgctcg atcgcccgcg ccttcagggc atccagcacc gcgtccagat cggccagcag
     3901 caggtccgcc gcctgggtca actgcaccga caggcaggtg tccagcacgt cggaactggt
     3961 catgccctgg tgcacgaagc ggctgtccgg cccgacggat tccgccagcc aggtcaggaa
     4021 cgcaatcacg tcgtgccgcg tttcgcgctc gatcgcgtcg atccggtcga tatcggcttg
     4081 gctgatctgc gccgcggccg cgccgccgcg ctcgcggatg gtgcgcgcgg cactctccgg
     4141 aatcgtgccg atgcgggcca tgccttccgc ggccagtgcc tcgatttcga accagatgcg
     4201 aaaccggttg gcgggcgccc agatggcatc catctcgggt ctggtatagc gaggaaccat
     4261 ccgtgccgcc tctgattgca attcggcacg ccagtacctc tccaggccgc cgcgtggcaa
     4321 gcgccggccg tcagcacagc gaggaccccg gctgaatgga tattagcctg ctgagccagg
     4381 acctctcggc gctggcgcag gtcgtgctga tcgacgtgac cctggccggc gacaacgccg
     4441 tggcggtcgg catggcggtg gccggactgc cgccgcggca gcgccgcccg gcggtgatgc
     4501 tgggcgtcgc cggcgcgacg gtggtccgcg tcgcgctggg tgccgtcgcg ctggaactgc
     4561 tcgcggtcat cggcctgctg ctggccggcg gcctgctgct gctgtgggtg tgctggaaga
     4621 tgttccgcga attgcgccac agcgccgccg cccgcaccag tctcgaccgg ccgcacaaga
     4681 cgctcggcca ggccatgctg cagatcgtcc tggccgacct gtccatgagc ctggacaacg
     4741 tgctggcggt ggccggcg
//
LOCUS       DASSZH010000266         2535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1455309, whole genome shotgun sequence.
ACCESSION   DASSZH010000266 DASSZH010000000
VERSION     DASSZH010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455309"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            326..625
                     /locus_tag="VFN42_05740"
     CDS             326..625
                     /locus_tag="VFN42_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996151.1"
                     /translation="MTVAEFHASREQPEPPPGSSLALQTLWWDAKGDWNRAHACAQQD
                     EGPTGSAVHAYLHRKEGDLSNAGYWYRRAGRPVFAGTLNDEWTALAEELLPGATA"
     gene            complement(622..>2535)
                     /locus_tag="VFN42_05745"
     CDS             complement(622..>2535)
                     /locus_tag="VFN42_05745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8996152.1"
                     /translation="PDGPLERWIAAQPPWSDFPFVLLSLRAPLPQRSSREVARLAELG
                     NVAVLERPLHPLTLVSAVRAAMRARRRQREAEAFLAERERQSTALRESEARFRAIAEG
                     MPQPIWSARADGMHDYFNTRWQEVTGVTPPVGGTGDGPAWMPWAGLLHPEDRDAAEQA
                     WRQVLDFGGAFRTEFRLRTAAGGYRWYLGRALPVRDELSGTIVRWFGSCTDIDDAVNA
                     RETLARSREELERLVDERTVSLQREMLEREKAEAALAQAQKMEAVGQLTGGVAHDFNN
                     LLTAVLASLEMISSRSTDERVLRFAANAQRAAERGARLTQQLLAFSRRQRLRPESVDM
                     NGLIRGMNELVTRAIGASVQFESRLSDDLPPAYVDPTQLELVLLNLAINARDAMPSGG
                     RLTVETFALQAPPDDLADELPPGSYVAMAVSDTGTGMPPEVQARAFEPFFTTKEMGKG
                     TGLGLSQVYGFVRQSGGTVRLRSTVGQGTRVSIYLPEAAALPEIRQEHPPAERPAATG
                     RATILVADDDENVRELAVAMLEELGYRVIAAEDGEVALRLIQRGEPFDLLVADVVMPG
                     LSGVEVVQRARKAGRQLRVLFATGYADLAVYREGLEGEDMIRKPYRMTELAERVERAL
                     AVYPAHGPSGAVN"
BASE COUNT          411 a          926 c          803 g          395 t
ORIGIN      
        1 tacatcgcaa atgatgtcgg gcaactccag cccggacaga gcccggaact cgctgacttg
       61 ctcgacttgg ctgacctcgc tttggagctg agagagcagc gcctggattt gggtgattgt
      121 gtccttcata cggacaggtt agcagacgat tccgctccgc gcacctgtcg gtagccatga
      181 atggaccgga gtccgctatc cagccgcctc atcgagggct atggcgtaga cgtagtgttc
      241 atcatcgtcg ggcgtggccc gccacatcag agccaacggc gcgttccgga ccgtgcgcaa
      301 accaccgcac atccgggagt caccgatgac cgttgccgaa ttccatgcct cacgcgagca
      361 gccggaaccg ccgccgggca gcagcctggc cctgcaaacc ttgtggtggg acgccaaggg
      421 cgactggaac cgcgcccatg cctgcgcgca gcaggacgag ggtccgaccg gttcggccgt
      481 gcacgcctac ctgcatcgca aggaaggcga cctgtcgaac gccggctact ggtaccgccg
      541 cgccggccgc ccggtcttcg ccggaacgct gaatgacgaa tggacggcgc tggcggagga
      601 gctgctgccg ggcgcaaccg cctagttcac tgctccgctt ggtccatggg ccgggtagac
      661 ggccagcgca cgctccaccc gttccgcaag ctcggtcatc cgatagggct tgcggatcat
      721 gtcctcgcct tccagcccct cgcgatagac cgccaggtcg gcataaccgg tggcaaacag
      781 cacgcgcaat tgccgaccgg ctttgcgcgc acgctgcacc acctccaccc cgctcaagcc
      841 cggcatcacc acgtcggcga ccagcagatc gaatggctcg ccacgctgaa tcagccgcag
      901 cgccacctcg ccgtcctccg ccgcaatcac ccggtagccg agttcctcca gcatcgccac
      961 ggcgagttcg cgcacgttct catcatcatc cgccaccagg atggtggcac ggccagtggc
     1021 cgccggacgt tcggccggcg gatgctcctg ccggatttcc ggaagcgccg cggcctccgg
     1081 caggtagatg ctgacccgcg tgccctgccc caccgtactg cgaagccgca cggtgccgcc
     1141 ggactgccgg acaaagccgt acacctgcga aagacccagg ccggtgccct tgcccatttc
     1201 tttcgtggtg aagaacggct cgaacgcgcg cgcctgcacc tccggcggca tgccggtccc
     1261 ggtgtcgctg accgccatcg ccacatagga cccaggcggg agctcgtcgg ccagatcgtc
     1321 gggcggggcc tgcaaggcga aggtctccac cgtcagccgg ccgccggagg gcatggcgtc
     1381 gcgcgcattg atcgccaggt tcagcagcac cagctcgagc tgcgtcggat ccacgtaggc
     1441 cggcggcaga tcatccgaga gccggctttc gaattgcacg gacgcgccaa tcgcccgcgt
     1501 caccagctcg ttcatgccgc ggatcaatcc gttcatgtcc acggattcgg gccgcagccg
     1561 ctgccggcgc gagaaggcca gaagctgttg cgtcagtcgt gcgccgcgct ccgccgcgcg
     1621 ctgggcgttc gcagcgaacc gcagcacgcg ttcatccgtg ctgcggctgc tgatcatctc
     1681 caggctggcg agcaccgcgg tcagcaggtt gttgaaatcg tgcgccacgc cgccggtcag
     1741 ttggccgacc gcttccatct tctgcgcctg ggccagcgct gcctcggcct tctcgcgctc
     1801 cagcatttcg cgttgcaggc tgacggtacg ctcgtccacc agccgttcca gctcttcccg
     1861 gctgcgggcc agcgtttcac gcgcgttcac cgcatcatcg atatcggtgc agctaccgaa
     1921 ccagcgcacg atcgtgcccg acagctcgtc ccgcaccgga agggcgcggc cgagatacca
     1981 gcgatacccg cccgctgcag tgcgcaggcg gaactccgtg cggaacgcac caccgaaatc
     2041 cagcacctga cgccaggcct gctccgcggc atcccggtct tccggatgca gcaggccggc
     2101 ccagggcatc caggccggcc catcgccggt tccgccgacc ggtggggtaa cgccggtcac
     2161 ttcctgccag cgcgtattga agtagtcgtg catgccgtcg gcacgggcgg accagatcgg
     2221 ctgcggcatg ccttcggcga tcgcgcgaaa ccgcgcttcg ctttcgcgca gcgcggtgga
     2281 ctggcgttcg cgctccgcca ggaacgcctc cgcttcgcgc tgccgccggc gcgcgcgcat
     2341 cgcggcgcgc accgcactga ccagcgtcag cggatgcagc ggccgttcca gaaccgcgac
     2401 gttgccgagc tccgccaagc gggccacctc ccggctggag cgctgcggca gcggcgcgcg
     2461 caggctgagc aggacgaagg gaaagtccga ccacggtggt tgcgccgcga tccaccgctc
     2521 cagcggccca tccgg
//
LOCUS       DASSZH010000267         2654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_57416, whole genome shotgun sequence.
ACCESSION   DASSZH010000267 DASSZH010000000
VERSION     DASSZH010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57416"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..1396
                     /gene="zapE"
                     /locus_tag="VFN42_05750"
     CDS             236..1396
                     /gene="zapE"
                     /locus_tag="VFN42_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HET8996153.1"
                     /translation="MDVPTQPDTARGPLAAYHSLRASGQLVADAGQEQAVRQLQMLWQ
                     QLRGYDPSPRHTPAGLLARLLHRHGNGQGHGGPTGLYLVGEVGRGKSMLMDLFFAAAD
                     VPRKQRIHFHRFMQNAHKRVHAYKLAHPDADDPIPPLADSIASESALLCFDEFQVHDI
                     VDALILGRLFQALFDRGVVVVATSNTPPDDLFAGQPGRDAFLPFIAILKQHMAVFEMD
                     AGRDFRRRRMRGVRTWHVPADAAADRALDDAFEQLTRGAVPKTETLQVMGRNVTVPLA
                     AEGVARFDFDALCGTALGAGDYLALASRYHTLILDGIPRLSPDNYDVARRFITLVDTL
                     YDHRVNLIASAAAMPDQLYRYGENAKMFERTASRLDEMQGKEWLDLPHLREA"
     gene            complement(1401..2009)
                     /locus_tag="VFN42_05755"
     CDS             complement(1401..2009)
                     /locus_tag="VFN42_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996154.1"
                     /translation="MNEVRAIHISGYERESRDFYRTPAWVTAALLRHVTLRGPVWEPC
                     CGDGAMATVLADHGHQVVASDIVDRGYGRPGVDFFTCQRFPTGCAAMVTNPPYGEAGP
                     EQQAMGFTRAIMPRFVRHALDLTQQANGQLALLVRLQWSAGKNAANLISSGPLDAMVV
                     LTKRIRWFDMGPKTNNGQHHHCWLFWDYQRNSACPPRIVFAG"
     gene            2155..2649
                     /locus_tag="VFN42_05760"
     CDS             2155..2649
                     /locus_tag="VFN42_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET8996155.1"
                     /translation="MLKPHFEQFAAYNRWANQRLYDDAERQPEEARKRELGLFFHSLH
                     GTLNHILVADRVWLRRLTGEGPQPERLNQILFDDFAALRAAREHEDARIIRFVGGLSE
                     ADLDATFSYHTMSGKAQEQRRAETLAHFFNHQTHHRGQAHAALTILGMAEPAPLDLLA
                     MQRG"
BASE COUNT          420 a          910 c          902 g          422 t
ORIGIN      
        1 gcgatcgatt tcatcaacca gacgatgggg cccggcgcgc gggtggccgt ggaactgacg
       61 ctggaggcgg cgaaggcgct cagcggcgcc ctggcgcggg cgatcacggc ggcggagatc
      121 gaggagagcg ggcgccggcg cggctgactg cgccggccgc gtccgcgcca taccgcccat
      181 ctgatggaag aggctggtcg cgccacccag ggcacgctat ctgaattctc ccgccatgga
      241 tgttcccacc caacccgata ccgcgcgcgg cccgctggcc gcgtaccact ccctgcgtgc
      301 ttcaggccag ctcgtggccg atgcggggca ggagcaggcc gtgcggcagt tgcagatgct
      361 gtggcagcaa ttgcggggct atgacccgtc gccgcggcac acgccggctg gcctgctcgc
      421 ccggctgctg catcggcatg gcaacggcca ggggcatggc gggccaaccg gactgtacct
      481 ggtcggcgaa gtgggccgcg gcaaatccat gctgatggac ctgttctttg ccgccgccga
      541 tgtgccgcgc aagcagcgca tccacttcca ccgtttcatg cagaacgcgc acaagcgggt
      601 gcatgcctac aagctggcgc atccggacgc cgatgatccg attccgccgc tggccgacag
      661 catcgccagc gaatcggcac tgctgtgctt cgacgaattc caggtgcacg acatcgtcga
      721 tgcgctgatc ctgggccgct tgttccaggc gctgttcgat cgcggcgtgg tggtggtggc
      781 gacctccaac acgccaccgg acgacctgtt tgccggccag cccggccgcg acgccttcct
      841 gcccttcatt gccatcctga agcagcacat ggcggtgttc gagatggatg ccggccggga
      901 tttccgccgc cggcggatgc gcggcgtgcg cacctggcac gtgccggccg acgccgcggc
      961 cgaccgggcg ctggatgacg cattcgagca gttgacccgc ggcgcggtgc cgaaaaccga
     1021 gacgctgcag gtgatgggcc gcaacgtgac ggtgccgctc gccgccgagg gcgtcgcccg
     1081 gttcgacttc gatgcgctgt gcggcaccgc actcggcgcc ggcgattacc tcgcgctggc
     1141 cagccgctat cacaccctga tcctggatgg cattccccgc ctgtcgccgg acaattacga
     1201 cgtggcgcgc cgcttcatca cgctggtgga cacgctgtat gaccaccggg tgaacctgat
     1261 cgcttccgcc gcggcgatgc cggaccagct ctatcgctac ggcgagaacg cgaagatgtt
     1321 cgagcgcacc gcctcacggc tggacgagat gcagggcaag gagtggctgg acctgccgca
     1381 cctgcgcgag gcgtgaggcg tcagccggca aacacgatcc gcggcgggca ggcgctgttg
     1441 cgctggtaat cccagaacag ccagcagtga tggtgctggc cgttattggt cttcggcccc
     1501 atgtcgaacc agcggatgcg cttcgtcagc accaccatgg cgtcgagcgg tccggagctg
     1561 atcaggttgg ccgcgttctt tcccgcgctc cattgcagcc ggaccagcaa tgcgagctgg
     1621 ccattggcct gctgcgtcag gtccagcgca tgccgcacga aacgcggcat gatggcgcgg
     1681 gtgaagccca tcgcctgctg ctccggcccg gcttcgccat agggcggatt ggtcaccatg
     1741 gcggcgcagc cggtggggaa acgctggcag gtgaagaaat ccactccggg ccggccatag
     1801 ccgcgatcca ctatgtcgct cgccaccacc tggtgcccgt gatcggccag caccgtggcc
     1861 atggcgccgt cgccgcagca tggctcccat accgggccgc gcagcgtgac gtgccggagc
     1921 agcgccgcgg tcacccaggc gggggtgcgg tagaaatcgc ggctctcgcg ttcatagccg
     1981 gaaatatgga tggcccggac ttcgttcatg cggtgcggtt cccagcaagt cggcccttac
     2041 ttgatactat cgccgccgag tcgggctatc ccccgccccg gccggatcgc cgccacttcc
     2101 gctccgaggc ggtctgcggc aacaatccgg gccggtcgtc acggagggct ccgcatgctg
     2161 aagccgcatt tcgagcagtt cgccgcctac aaccgctggg ccaaccagcg cctgtacgat
     2221 gatgccgaac gccagccgga ggaggcgcgc aagcgcgagc tgggcctgtt cttccacagc
     2281 ctgcacggca cgctgaacca catcctggtg gcggaccgcg tctggctgcg ccggctgacg
     2341 ggggaggggc cgcagccgga acggctcaac cagatcctgt tcgacgattt tgccgcgttg
     2401 cgcgccgctc gggagcacga ggatgcgcgc atcatccgct tcgtcggcgg gctgagcgag
     2461 gcggatctgg acgcgacgtt cagctaccac accatgtccg gcaaggcgca ggaacagcgc
     2521 cgcgccgaaa ccctggcgca tttcttcaat caccagacgc atcatcgcgg ccaggcgcat
     2581 gccgcgctga ccatccttgg catggcggag ccggcgccgc tggacctgct ggcgatgcag
     2641 cgcggctgag gcgt
//
LOCUS       DASSZH010000268         2629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1028908, whole genome shotgun sequence.
ACCESSION   DASSZH010000268 DASSZH010000000
VERSION     DASSZH010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028908"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VFN42_05765"
     CDS             <1..631
                     /locus_tag="VFN42_05765"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8996156.1"
                     /translation="FVDAFWTSLWLGTLSATLAVTITVPAALAIARHRFPGRETIATL
                     MQTPLMIPPAVLGIAFLRFFTITGISGTFLGLVMSHVIIIMPYALRLALSASTSLDRQ
                     AEHAAASLGASAWATFRRVTLPMILPGVVSGWVLAFITSFDEVTMTVFIASPRTTTLP
                     VRLFMYIQDNIDPLVAAVSAGLIGLTAVGMVVLDRIYGLDRLLIGRGNQ"
     gene            638..1768
                     /locus_tag="VFN42_05770"
     CDS             638..1768
                     /locus_tag="VFN42_05770"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8996157.1"
                     /translation="MTQPAPTSFDVAVVGGGLVGAAIAYGLARLGKRVAVLDEGDIAY
                     RASRGNFALVWLQSKGLGMPAYAGWTKRSSDMWTGFARELHDESGIDVAFQRPGGFQL
                     ALSDRELEARASTLRRLHNQPDMVPYPYEILDHAGIARMLPEIGPEVVGGSYCPLDGH
                     CNSLRLLRALHIGMERRGVHYLAEQRVEQIDHAGGGFRLRHNRGELHAERVVLAAGNG
                     NARLAPMVGLRAPVRPQRGQIVVTERVRPFLNYPVSTVRQTDDGTVMLGDSLEEAGFD
                     DTVGTAVTSVIADRATRMFPLLRPLNVVRSWAALRVMTQDGFPIYDQSEQCPGAFLAT
                     CHSGVTLAASHALALAPHLAAGSLPAEFSAFSARRFVVPASV"
     gene            1761..2045
                     /locus_tag="VFN42_05775"
     CDS             1761..2045
                     /locus_tag="VFN42_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET8996158.1"
                     /translation="MSESVAEEITVSIDGAPAVVRAGDSVAAALLAAGHLQCRTTPVS
                     DAPRAPYCLMGVCFECLVEIDGIGSRQACMVRVTPGMRIATQHGRREAGR"
     gene            2042..>2629
                     /locus_tag="VFN42_05780"
     CDS             2042..>2629
                     /locus_tag="VFN42_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8996159.1"
                     /translation="MNSDIKPQYQLAVIGAGPAGLAAAATAARHGLDTVLLDEQPAPG
                     GQIYRAITETRLRDPALLGADYWHGFELVAAFRASGAQHLAGATVWSVSRDREIGVSI
                     AGRAQLFRAERVILATGALERPFPIPGWTLPGVMTVGAAQILLKTSGMVAQGRVVLAG
                     TGPLLWLLAAQYLRAGASIAALLDTTPSDNWRAALP"
BASE COUNT          383 a          898 c          878 g          470 t
ORIGIN      
        1 cttcgtcgat gccttctgga ccagcctgtg gcttggcacc ctgtccgcca ccctggccgt
       61 caccatcacc gtaccggccg cactggccat cgcccggcac cgatttccag ggcgcgagac
      121 catcgccacg ctgatgcaga ccccgctgat gatcccgccc gcggtgctgg gcattgcctt
      181 cctgcgcttc ttcaccatca ccggcatcag cggcacgttt ctcggcctgg tgatgagtca
      241 tgtcatcatc atcatgcctt acgcgctacg ccttgcgctg tcggcttcca ccagcctcga
      301 tcgccaggcg gagcacgccg cggcatcgtt gggcgcctcc gcctgggcga cgttccgccg
      361 cgtgacgctg ccgatgatcc tgcccggcgt ggtcagcggc tgggtgctgg ccttcatcac
      421 cagcttcgac gaagtgacca tgacggtgtt cattgcctcc ccgcgtacca ccacgctgcc
      481 cgtgcggctc ttcatgtata tccaggacaa tatcgatccc ctggtcgccg cggtatccgc
      541 cggcctaatc ggcctgacag cggtggggat ggtggtgctg gaccgcatct atggactcga
      601 ccggctgctg atcggtcgcg gcaatcagtg aatgcctatg acgcaacctg ccccaacgag
      661 ttttgatgtc gccgtggtcg gcggtggcct ggtcggtgcc gcaatcgcct atggactggc
      721 gcgcctcggc aagcgcgtgg ccgtgctgga cgaaggcgac atcgcctatc gtgcctcacg
      781 cggcaacttc gctttggtct ggctgcagag caaaggcctc ggcatgccgg cctatgccgg
      841 atggaccaag cgttcgtccg acatgtggac cggattcgcc cgcgagttgc atgatgaatc
      901 cggcatcgac gtcgccttcc aaagaccagg cggtttccag cttgcgctgt cggatcgcga
      961 gctggaagcg cgtgccagta cattgcgccg gttgcacaac cagccggaca tggtgccgta
     1021 tccgtacgag atcctggacc acgccggaat cgcccgcatg ctgccggaga tcggtcccga
     1081 ggtcgtcggc ggcagctact gtccgctcga cgggcactgc aactcgctgc gcctgctgcg
     1141 tgcgctgcac atcggcatgg agcggcgcgg cgtacactac ctggcggaac agcgagtcga
     1201 gcagattgac cacgccggtg gcggcttccg cctgcgccac aaccgcgggg aactgcatgc
     1261 tgagcgcgtg gtgctcgccg caggaaatgg caatgcgcgg ttggcaccga tggtgggctt
     1321 gcgggcgccg gtgcgtccgc aacgcggcca gatcgtcgtc accgagcgcg tgcgtccctt
     1381 cctgaactat ccggtatcca cggtgcgcca gaccgacgac ggcacggtga tgctgggcga
     1441 ttcgctggag gaagccggct tcgacgacac ggtcggcacc gccgtcacct cggtgatcgc
     1501 cgatcgggcc acgcgcatgt tcccgctact gcgtccgttg aatgtcgtgc gtagctgggc
     1561 ggcgctgcgt gtcatgaccc aggacgggtt cccgatctac gaccagtcgg aacagtgtcc
     1621 cggcgccttc cttgccacct gccacagcgg cgtgacgctc gcggccagcc acgcgctggc
     1681 gctggcgccg cacctggcgg cgggcagcct tcccgccgaa ttcagcgcgt tcagcgcacg
     1741 gaggttcgtt gttccggcga gtgtctgagt ctgttgccga ggaaatcacg gtatccatcg
     1801 acggcgcacc ggccgtcgtg cgggccggcg acagcgtcgc cgcggcgctg ctcgccgcag
     1861 gacacctgca atgccgcacc acgccggttt cggacgcacc gcgcgcgccc tattgcctga
     1921 tgggcgtctg cttcgagtgc ctggtggaga tcgatggcat cggcagccgg caggcctgca
     1981 tggtgcgcgt gacgccgggg atgcgcatcg cgacacagca cgggcggcgc gaggcaggcc
     2041 gatgaattcc gacatcaagc cgcagtacca gcttgcggtg atcggcgccg gcccggcggg
     2101 cctggccgcc gccgcgaccg ccgcgcggca cggcctggac acggtgctgc tggacgagca
     2161 gccggcgccc ggagggcaga tctatcgcgc gatcacggaa acccgtttgc gcgaccctgc
     2221 cctgctgggc gccgattact ggcacggttt cgagctggtc gcagcgtttc gcgccagcgg
     2281 ggctcaacat ctggccggtg caacagtgtg gagcgtgtcg cgtgaccgcg aaatcggcgt
     2341 ctcgattgcc ggcagggcgc aactgtttcg cgctgagcgg gtgatcctcg ctaccggggc
     2401 gctggaacgg ccgttcccga tcccggggtg gaccttgcct ggcgtcatga ccgtgggcgc
     2461 cgcgcagatc ctgctgaaga cctccggcat ggtggcgcag gggcgtgtcg ttctggccgg
     2521 caccggtccg ctgctgtggt tgctcgccgc gcaatacctg cgtgccggcg ccagcattgc
     2581 cgcattgctg gacaccacgc ccagcgacaa ctggcgcgcc gcattgccg
//
LOCUS       DASSZH010000269         6286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_671056, whole genome shotgun sequence.
ACCESSION   DASSZH010000269 DASSZH010000000
VERSION     DASSZH010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671056"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VFN42_05785"
     CDS             <1..277
                     /locus_tag="VFN42_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8996160.1"
                     /translation="AMVVAPEPDLIILDEPTAGMSAEETHRAAGLIRELNREHAIVVV
                     EHDMQFIRAIARTVTVFHQGRVLMEDAAARVFGDPRVRDVYLGKAVA"
     gene            274..996
                     /locus_tag="VFN42_05790"
     CDS             274..996
                     /locus_tag="VFN42_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8996161.1"
                     /translation="MIAVADLVSGYGRIGVLDGISLDVAAGECVGILGHNGMGKSTLL
                     RTIIGHLPAWRGSIRLDGAPIERLAPHERARAGIGYVPQGRQIFPGLSVRENLHFAAV
                     AKRRPREAAEAEMLEEFPELRRLLDRPGGALSGGEQQLLALARALAGTPRLLLLDEPT
                     EGIQPSIVQAMIARIADLRRRRGLAVLLVEQNLDFIRGLADRVLLIHRGRILRALDRE
                     AIADPSLEQEFIGAGPTRNPDH"
     gene            1010..2539
                     /locus_tag="VFN42_05795"
     CDS             1010..2539
                     /locus_tag="VFN42_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET8996162.1"
                     /translation="MTLSTVKVPTIEQLRDVAADLGMSFTEEDLAAHMAALVGGVAAY
                     NIVDQMPDELPAVKYPRLPGRRPAGEENKHNAWYVKTRVEGASGGKLKGKTVVLKDNI
                     CLAGVPMMNGAATLEGYVPDVDATVATRVLDAGGTIVGKAVCEYFCFSGGSHTSASGP
                     VHNAHRMGYSAGGSSSGSATLVALGEVDMALGGDQGGSIRMPSAYSGTYGMKPTHGLV
                     PYTGIMPIELTIDHTGPITGTVEDNALLLEVLAGPDGLDPRQYGGQPQEYRAGLGKGV
                     AGLRIAVVPEGFGHPQSMAKVDDLVRAAAERFKALGATVDTVSIPMHLLGPAIWLPVA
                     AEGATMQMMLGNGFGFNWQGLYVTSMLDFHSAWRQRADELSDTLKNTMLLGHYMVKHY
                     RGHYYAKAQNLVRRLRAAYDAVLANYDLLLMPTVPLTATPLPEANAPIPEILQRAFEM
                     LPNTSPFDCTHHPAMSVPCGMADGLPVGVQLVGKLYDESTIYRAAAAFEKAADWKKIT
                     A"
     gene            complement(2597..3313)
                     /gene="queC"
                     /locus_tag="VFN42_05800"
     CDS             complement(2597..3313)
                     /gene="queC"
                     /locus_tag="VFN42_05800"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HET8996163.1"
                     /translation="MREDADGALVLFSGGQDSATCLAWALDRFARVETLGFDYGQRHR
                     VELDCRATFRAGLAQLFPGWAARLGADHTLPLQALAAVSDTALTRETEIRLQEDGLPN
                     TFVPGRNLLFLTFAAALAYRRGLRHIVAGMCETDYSGYPDCRDDTIKALQVAINLGME
                     RRFVLHTPLMWLTKAATWQLAEELGGRPLIDLLLEDTHTCYLGDRTHRHAWGYGCGRC
                     PACDLRQKGWAEYTSRHPAA"
     gene            complement(3375..4349)
                     /locus_tag="VFN42_05805"
     CDS             complement(3375..4349)
                     /locus_tag="VFN42_05805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HET8996164.1"
                     /translation="MKAWRVEQITESGDMHLADLPTPQPGAGQYLVRVEAAGVNFLDT
                     LMIRGRYQKKPPLPFTPGVECAGTILAAGEGCKLAPGRRVLGSVEGGGFAECALMPAR
                     AALEVPEGMAAAEALTLLGVNYPTSYYALHDRAGLRAGETVLVHAAAGGVGSAAVQLA
                     KAAGARVIATAGSAEKLEVCRRLGADEALSYAEGDWVEAVRGLTGGRGVDVIYDPVGG
                     EVGEQSLRVLAWRGRYLVIGFAAGGIPKLPANRLLLMNAAALGVFWGEVLNREPATAE
                     AVRAALVSLYRRGAVPPLIGGRYTLAEAPAALAALAGRATVGKLVLEP"
     gene            complement(4445..4831)
                     /locus_tag="VFN42_05810"
     CDS             complement(4445..4831)
                     /locus_tag="VFN42_05810"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HET8996165.1"
                     /translation="MIIVYDGECPFCASYVRLMALKKKVGAVELVDARSNHHSVRQLQ
                     GRGYDLDEGMAVIFGDAVYYGPDAVVLISEITEDGGWHQRLLSGLLRNPARARRLYPA
                     LKLGRRIALRLLGKPGISPLRTSGGE"
     gene            complement(4848..>6286)
                     /locus_tag="VFN42_05815"
     CDS             complement(4848..>6286)
                     /locus_tag="VFN42_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996166.1"
                     /translation="TALLSQARFVTVERPLIRLWQLIGALSALYGLAHYPLFPPIPGD
                     TTAAALYALLVTLWLASLACGVLCFRIPSLAMVPPAFLLWSALAASRITGLRHDKLLD
                     IAPLPEIAFCIGLGLAISQVQAKVARGDEGTREHFARVLLLVAIGIHFANYFWSAVTK
                     ASLDGPPLAWVTQNNPVRLFLVAWDNDHLFFAENPAVFRFLSNVIDKSHLLTNVLILS
                     AQALAVAAFFLPKRLILLLLTVYDLMHASIIVVAGANFWPWILLNVAVGIVVASREFE
                     RPKPLAAAGAALFIVISPHFVNVAWLGWYDSGANNKLFFEAEDTAGRRYYVPTNLFAF
                     YSYPLAHMDYGSPQPETAFSVGSPSGWVQSYRLFQAGLQCNPAALTQPDSHQLKARPM
                     DPHLDQFVRDYHHMVLALQRYIGALPYDACPHHLYIGWGRGRDFGQLDKRDIRAYIYR
                     RESVCMSWQDGLPVRKILSTAEHRIDVQ"
BASE COUNT         1076 a         2180 c         2097 g          933 t
ORIGIN      
        1 cgcgatggtg gtggcgccgg aacccgacct gatcatcctc gacgagccga ccgcgggcat
       61 gagcgccgag gaaacccacc gcgccgccgg cctgatccgc gaactgaacc gcgagcacgc
      121 gatcgtcgtc gtcgagcacg acatgcagtt catccgcgcc atcgcccgca ccgtcaccgt
      181 gttccaccag gggcgcgtgc tgatggagga cgcggcggcg cgcgtgttcg gcgacccgcg
      241 cgtgcgcgac gtctatctcg gcaaggcggt cgcatgatcg cggtcgcgga tctcgtttcc
      301 ggctatggcc gcatcggcgt gctggacggc atctcgctcg atgtcgcggc cggcgagtgc
      361 gtcggcatcc tcggccacaa cggcatgggc aaatcgacgc tgctgcgcac gatcatcggc
      421 cacctgccgg cgtggcgcgg cagcatccgg ctggacggcg cgccgatcga gcggctggcg
      481 ccgcacgagc gcgcgcgtgc cggcatcggc tacgtgccgc agggacggca gatctttccc
      541 ggcctttcgg tgcgggagaa cctgcatttc gccgccgtcg ccaaacgccg gccgcgcgag
      601 gcggcggagg cggagatgct ggaggagttc cccgaactcc gccgcctgct cgaccggccc
      661 ggcggcgcgc tgtcgggcgg ggagcagcaa ttgctcgcgc tggcccgcgc gctggccggc
      721 acgccgcgcc tgctgctgct ggacgagccg accgagggca tccagccgtc gatcgtgcag
      781 gcgatgatcg cgcgcatcgc cgacctgcgg cgccggcggg ggctggcggt tctgctggtc
      841 gagcagaatc tcgatttcat ccgcggcctt gccgaccgcg tgctgctgat ccatcgcggc
      901 cgcatcctgc gcgcgctcga ccgcgaggcc atcgccgacc cgtcgctgga acaggagttc
      961 atcggcgccg ggccaacccg caaccctgac cattgaggga gaccgatcca tgacgctgtc
     1021 caccgtgaag gtgccgacga tcgagcagtt gcgcgatgtc gcggccgatc tcggcatgtc
     1081 gttcaccgag gaggatctcg ccgcgcacat ggcggcgctt gtcggtggcg tcgccgccta
     1141 caacatcgtc gatcagatgc cggacgaact tccggcggtg aaatacccgc gcctgcccgg
     1201 ccgtcgcccc gccggcgagg agaacaagca caacgcctgg tacgtcaaga cgcgggtgga
     1261 gggtgcatcc ggcggcaagc tcaagggcaa gaccgtcgtg ctcaaggaca atatctgcct
     1321 cgccggcgtg ccgatgatga acggcgccgc cacgctggaa ggctacgtgc cggacgtgga
     1381 cgcgacggtc gcgacgcgcg tgctggacgc cggcggcacc atcgtcggca aggcggtgtg
     1441 cgagtatttc tgcttctccg gcggcagcca caccagcgcc tccggcccgg tccacaacgc
     1501 gcaccgcatg ggctattccg ccggcggctc ctcctccggc agcgcgacac tggtggcgct
     1561 gggcgaggtg gacatggcgc tcggcggcga ccagggcggc tcgatccgta tgccctcggc
     1621 ctattccggc acctacggca tgaagccgac gcacgggctg gtgccctaca ccggcatcat
     1681 gccgatcgaa ctgaccatcg accacaccgg gccgatcacc ggcacggtcg aggacaacgc
     1741 gctgctgctg gaggtgctgg cgggtccgga cggactcgat ccgcgccagt atggcggcca
     1801 gccgcaggaa taccgcgcgg ggctgggcaa gggcgtggcc gggctgcgca tcgcggtggt
     1861 gccggagggc ttcgggcatc cgcagagcat ggcgaaggtg gacgacctcg tgcgggcggc
     1921 ggcggaacgg ttcaaggcgc tgggggcgac ggtcgatacg gtctccatcc cgatgcacct
     1981 cctggggccg gcgatctggc tgccggtggc ggcggagggg gcgaccatgc agatgatgct
     2041 gggcaacggc ttcggcttca actggcaggg cctgtatgtc acctcgatgc tggatttcca
     2101 cagcgcgtgg cggcagcgcg ccgatgagct gtcggacacg ctgaagaaca ccatgctgct
     2161 cggccactac atggtgaagc attaccgcgg gcattactac gccaaggcgc agaacctggt
     2221 gcgccgcctg cgcgccgcct acgacgcggt gctggcgaac tatgacctgc tgctgatgcc
     2281 gacggtgccg ctgacggcca ccccgctgcc ggaggcgaac gcgccgatcc ccgaaatcct
     2341 gcagcgcgcg ttcgagatgc tgcccaacac ctcgccgttc gactgcacgc accatccggc
     2401 gatgagcgtg ccgtgcggca tggcggacgg cctgccggtc ggcgtgcaac tggtcggcaa
     2461 gctctacgac gagagcacga tctaccgcgc cgccgccgcc ttcgagaagg ccgccgactg
     2521 gaagaagatc accgcctgaa cgcgtttccg cagcgcgcct ctcccgctcg tgcgggaggg
     2581 gcagcgcctg ccgcgactac gcggccgggt ggcgggacgt gtactccgcc catccctttt
     2641 gccgcaggtc gcaggcgggg caccggccgc agccatatcc ccaggcatgg cggtgcgtcc
     2701 tgtcgccgag gtagcaggtg tgcgtgtcct caagcagcag gtcgatcagc ggccggccac
     2761 ccaactcctc cgccaactgc cacgtcgccg ccttggtcag ccacatcagc ggcgtgtgca
     2821 gcacgaagcg ccgttccatg cccaggttga tcgccacctg gagcgccttg atcgtgtcgt
     2881 cgcgacagtc gggatagccg gagtagtccg tttcgcacat ccccgcgacg atgtggcgca
     2941 gaccacggcg ataggcgagc gccgcggcga aggtcaggaa cagcaggttg cggcccggca
     3001 cgaacgtgtt cggcaggccg tcctcctgca ggcggatctc cgtctcgcgg gtcagcgccg
     3061 tgtcggacac cgcggcgagc gcctgcagcg gcagcgtatg gtcggccccg agccgggcgg
     3121 cccagccggg aaaaagctgc gccagcccgg cacggaaggt tgcgcggcag tccagttcca
     3181 cgcggtgccg ctggccgtag tcgaagccca gcgtttccac ccgtgcgaac cgatcgagcg
     3241 cccaggccag acaggtcgcg ctgtcctgcc cgccgctgaa cagcaccagc gcgccgtcgg
     3301 cgtcttccct catctgcctt ccttcgcatg atgccggtcg gcggcgagac atccaacgcg
     3361 cgatcgccgg cggttcatgg ctccagcacc agcttcccga ccgtggcccg cccggccagc
     3421 gccgccagcg ccgccggggc ctcggcgagc gtgtagcgcc cgccgatcag cggcggcacc
     3481 gcgccccgcc ggtacaggga cacgagtgcc gcgcgcaccg cctcggccgt ggccggctcg
     3541 cggttcagca cctcgcccca gaacacgccc agcgccgccg cattcatcag cagcagccgg
     3601 ttagccggca gtttcgggat gccgccggcg gcgaagccga tcaccaggta gcggccacgc
     3661 caggccagca cgcgcaggct ctgctcgccc acctcgccgc ccaccgggtc atagatcacg
     3721 tccaccccgc gcccgccggt cagtccgcgc accgcctcga cccagtcgcc ctcggcgtag
     3781 ctcagcgcct cgtcggcgcc gaggcggcgg cagacctcca gcttctccgc actccccgcc
     3841 gtcgcgatca cccgcgcgcc cgccgccttg gccagttgca ccgccgcact gcccacgccg
     3901 cccgccgcgg catgcaccag caccgtctcg cccgcccgca ggccggcgcg gtcgtgcaga
     3961 gcgtagtagg aggtgggata gttgaccccc agcagcgtca gcgcctccgc cgccgccatc
     4021 ccctccggca cctccagcgc ggcgcgggcg ggcatcagcg cgcattcggc aaagccgccg
     4081 ccctccaccg agccgaggac gcggcgtccg ggcgcgagct tgcatccctc gccggcggcc
     4141 aggatcgtgc cggcgcattc cacgccgggg gtgaagggca ggggcggctt cttctggtag
     4201 cggccgcgga tcatcagcgt atcgaggaaa ttcacccccg ccgcctccac ccgcaccaga
     4261 tactggcccg cacccggctg cggcgtcggc aggtcggcaa ggtgcatgtc gccgctctcg
     4321 gtgatctgct cgacgcgcca ggctttcatg gttccgtcct ctcccgttcg ccccatgctt
     4381 gaccagccgg cgcccggccg caaccggggg cggcgatgcg gagggtgccc ggaatttctg
     4441 gaaactactc gccgccactc gtccggaggg gcgaaatgcc gggtttgcca aggagacgca
     4501 gggcgatccg gcgccccagc ttcaaggcgg ggtagaggcg tcgcgcccgc gccgggttac
     4561 ggagcagacc ggagagcagc cgctgatgcc atcccccgtc ctcggttatc tcggaaatca
     4621 agaccaccgc atcggggccg tagtagacgg catctccaaa gatcacagcc atgccttcgt
     4681 ccaggtcgta gccgcggccc tgaagctgcc gcacgctgtg gtggttgctc cgggcatcga
     4741 ccagttctac ggcacctacc ttctttttca gggccatcag ccggacgtag ctggcgcaga
     4801 acgggcactc gccgtcatag acgatgatca tgcccttggc cggtgatcta ctgtacatcg
     4861 atccggtgtt ccgcggtgga caggattttg cgcacgggca ggccgtcctg ccaactcatg
     4921 cacacggatt cacgtcggta gatgtacgcg cggatgtccc gcttatcaag ttgaccgaaa
     4981 tcccgccctc gtccccagcc tatatagaga tggtgcggac aggcatcata cggcagcgcg
     5041 ccaatatatc gttgcagcgc aagcaccatg tggtggtagt cccgcacgaa ctgatcaagg
     5101 tgtgggtcca tcggcctggc cttcaactgg tgcgaatcag gctgcgtgag ggctgctgga
     5161 ttgcattgca ggccggcctg gaacaggcga taagactgaa cccagcctga cggcgaaccg
     5221 accgaaaatg ccgtttcagg ctgaggggaa ccatagtcca tatgggccag agggtaggaa
     5281 taaaatgcaa atagattggt cggaacatag tatcgacggc cggcggtatc ctccgcttcg
     5341 aagaacaatt tattattggc gccgctgtca taccatccaa gccacgcgac attcacgaag
     5401 tggggtgaaa tcacaatgaa aagcgccgcg cccgccgccg ccaggggctt cggtcgctcg
     5461 aattcgcggc tcgcgacgac gatgccaacc gcaacgttga gcagaatcca aggccagaaa
     5521 ttcgctcctg ccacaacgat gatcgaggcg tgcatcaaat catagacggt cagcagcagg
     5581 aggatgaggc gcttcggaag gaaaaaggcc gccaccgcca gggcctgcgc ggagaggatc
     5641 aggacattcg tcagaaggtg cgatttgtct atgacattgc tcaggaacct gaatacggcc
     5701 ggattttcgg caaaaaataa atggtcgttg tcccacgcca ccaaaaacaa cctgaccgga
     5761 ttgttctgag tgacccaggc caggggcggc ccatccaggc tggccttggt gaccgccgac
     5821 cagaaataat tggcgaaatg gatgccgatt gcgacaagca gcaagacgcg ggcgaaatgc
     5881 tctcgcgtgc cttcgtcgcc ccgcgcgacc ttcgcctgca cctggctgat cgccagccca
     5941 agtccgatgc agaacgcgat ctccggcaac ggggcgatat ccaggagctt gtcgtgacgc
     6001 agcccggtga tccgcgaagc cgcgagggcg ctccacaaca ggaaggccgg cggcaccatc
     6061 gcgaggctgg ggatgcggaa acaaaggaca ccgcaggcca gcgacgccag ccagagcgtg
     6121 accagcaggg catagagcgc cgccgccgtc gtatcccccg gtatcggcgg gaacagcgga
     6181 taatgcgcga ggccgtaaag cgccgagagt gcgccgatga gctgccacag acggatcaag
     6241 ggccgctcga ccgtgacaaa ccgcgcctgc gacagcagcg ccgtca
//
LOCUS       DASSZH010000270         3654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1523523, whole genome shotgun sequence.
ACCESSION   DASSZH010000270 DASSZH010000000
VERSION     DASSZH010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523523"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VFN42_05820"
     CDS             <1..783
                     /locus_tag="VFN42_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET8996167.1"
                     /translation="VTRLEPLTISHQGVFPAVTLSFNLAPGVALGQAVAAVDQMTQRL
                     HAPDTLNGAFEGTAQAFQTSLTSMPLLLAAAILVVYIVLGMLYESYIHPITILSALPA
                     AGVGALIMLWACHYDLSVIAIIGIVLLIGIVKKNAIMMIDFALSAQRNEGKTPREAIR
                     EACLLRFRPIMMTTFSALFGSLPIALGQGAGSELRQPMGIAFIGGLLVSQWLTLYTTP
                     VVYLYLDRFATWLGGSYRQPRLAEMLGEAAQIDSVEQGRRWQ"
     gene            complement(861..1859)
                     /gene="trpS"
                     /locus_tag="VFN42_05825"
     CDS             complement(861..1859)
                     /gene="trpS"
                     /locus_tag="VFN42_05825"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET8996168.1"
                     /translation="MQRIFSGIQPSGIATLGNYLGAIRNWVALQDDHECIWCVVDLHA
                     ITEWQNPAELTRQTREMAAVLLACGIDPEKHILFLQSSVRAHAQLAWICNCIARIGWL
                     NRMTQFKDKAGKNRENASAGLYVYPNLMAADILAYHATRVPVGDDQRQHLELANDIAQ
                     KFNHDYGVDFFPAIEPLILGPAARVMSLRDGSRKMSKSDPSDQSRINLNDDAETIALK
                     VRRAKTDPEPLPSEPQGLANRPEARNLVGIYAALADADHGSVLREYGGQGFSAFKEAL
                     ASLMVEKLAPIAGETKRLLDDPAQLDAILRNGAERAAAIADPIVAEAERLVGFLRA"
     gene            2170..2883
                     /locus_tag="VFN42_05830"
     CDS             2170..2883
                     /locus_tag="VFN42_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HET8996169.1"
                     /translation="MSFSPDNPTGRLYQPYAAAPAIDLELRSFMLRVFNTMAGGLALT
                     GIVAYAAAQSGLYAALAGSPLIWLVMLAPLGLALLLGFRVGQMSPAAAQAAFWGYAGL
                     MGLSLAGIFLVYTGDSIARVFFISAGTFAATAAWGYTTRTDLSRFGSFLVMGLIGIIL
                     AGLVNLFIGSSALQFALSVIGVAVFTGLTAWDTQRLKAMYLENGGAGNLAILGALMLY
                     LDFVNLFVSLLQLLGVRRD"
     gene            2910..>3654
                     /locus_tag="VFN42_05835"
     CDS             2910..>3654
                     /locus_tag="VFN42_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HET8996170.1"
                     /translation="MDWLTDPQVWAGLLTLTALEVVLGIDNLVFIAVLVARVRPGHQA
                     LARRLGLGLALGTRLLLLAGISWIAQLTAAVFTVAGHGFSWRDLILIGGGLFLVWKGT
                     AEIHDRVEGESHAPGGAAPHAGLYGIVLQIAVLDIVFSLDSVITAVGMVAHLPVMIAA
                     VVIAMGVMLLAAGPTTEFVARHPTVKMLVFSFLLLIGMTLLADGFGMHVPKGYIYAAI
                     GFSAGVEVLNQLAGRRQRRRAGAVSNHRMH"
BASE COUNT          510 a         1242 c         1221 g          681 t
ORIGIN      
        1 gtgacgcggc ttgagccctt gacgatcagc caccagggcg tcttcccggc ggtcacgctg
       61 tcattcaacc tggcgccggg cgtggcgctc ggccaagccg tcgcggcggt cgaccagatg
      121 acccagcggc tgcacgcgcc ggacacgctg aacggcgcgt tcgaaggcac cgcgcaggcg
      181 ttccagactt cgctcacctc gatgccgctg ctgctcgcgg ccgcgatcct tgtcgtctac
      241 atcgtgctgg gcatgctcta cgagagctac atccatccga tcacgatcct gtccgccctg
      301 ccggcagcag gggtcggcgc actgatcatg ctgtgggcct gccattacga tctatcggtg
      361 atcgcgatca tcggcatcgt gctgctgatc ggcatcgtca agaagaacgc catcatgatg
      421 atcgacttcg cgctgtcggc gcagcgcaac gagggcaaaa ccccgcgtga ggcgatccgc
      481 gaagcctgcc tgttgcgctt ccggccgatc atgatgacga ccttctcggc gctgttcggc
      541 agcctgccta tcgctctggg gcagggcgcg ggatcggaat tgcgccagcc gatgggcatc
      601 gcgttcattg gcgggctatt ggtctcgcag tggctgacgc tgtacacgac gccggtcgtg
      661 tatctttatc tcgaccgctt tgccacctgg ctgggcggca gctatcgcca gccccggctg
      721 gccgagatgc tgggcgaggc agcgcagatc gacagcgtgg agcaggggcg gaggtggcaa
      781 taagccgcgg cgcggcggcc ggcctccggg tcatggccga agggccagcc gtccagctgt
      841 ttcgccggct gccggaatgt ctacgcccgc aggaagccca ccagccgctc cgcctccgcc
      901 acgatgggat cggcaatcgc cgccgcgcgc tccgcgccat tgcgcagaat cgcatccagc
      961 tgcgccggat cgtccagcag tcgcttggtc tcgccggcga tcggcgccag tttctccacc
     1021 atcaggctgg ccagcgcctc cttgaacgcg ctgaatccct ggccgccgta ttcgcgcaat
     1081 accgacccgt ggtcggcgtc cgccagcgcg gcgtagatcc ccaccaggtt gcgggcctcc
     1141 ggccggttcg ccagtccctg cggttcgctc ggtagcggct ccggatcggt cttcgcccgg
     1201 cggaccttca gcgcgatcgt ctccgcgtcg tcgttcaggt tgatccggct ctgatcggac
     1261 ggatcggact tcgacatctt ccggctgccg tcgcgcaggc tcatcacccg cgccgccggc
     1321 cccaggatca gcggctcgat cgccgggaaa aaatccaccc cgtaatcgtg gttgaacttc
     1381 tgcgctatgt cgttggccag ctccaggtgc tggcgctggt cgtcgcccac cggcacgcgc
     1441 gtggcgtggt aggccaggat gtcggccgcc atcaggttgg gatacacgta cagcccggcc
     1501 gaggcgttct cccggttctt gcccgccttg tccttgaact gcgtcatccg gttcagccag
     1561 ccgatgcgtg cgatgcagtt gcagatccag gccagctgcg catgcgcccg caccgaggac
     1621 tgcaggaaca ggatgtgctt ctccgggtcg atgccgcagg ccagcagcac ggccgccatc
     1681 tcccgcgtct gccgggtgag ttccgccggg ttctgccact cggtgatggc atgcaggtcc
     1741 accacgcacc agatgcactc gtggtcgtct tgcagcgcca cccagttgcg gatcgcgccg
     1801 aggtagttgc cgagggtggc gatcccggaa ggctggatgc cggaaaaaat gcgttgcatg
     1861 ggcacggtcg ccggtcagtg gtcgggcggc acagatggcc gatccccgcc caaggcgcaa
     1921 gcgtgcggtc cccatggcac agcgagcggc gcagcgggga gcgtggcacg gtcttgcgcg
     1981 gcgacccccg cagccccatc tgattgccgt tacaacacga tccgggcccc tctggcggga
     2041 cgtcggccca tggccggcca acccgcgatg tcggatcacc gcacggaatg cggcgatcga
     2101 tccggcccgg cttgcagccc ttcatcgctc ctccgtgcgt cgttcgatga cacatgacag
     2161 gagcacgcga tgagtttctc tcccgacaac ccaaccggcc gattgtatca gccctatgct
     2221 gccgcgccgg cgatcgacct ggagctgcgc agtttcatgc tgcgcgtgtt caacaccatg
     2281 gccggcggcc tcgcgctgac cgggatcgtc gcctatgcgg cggcgcagtc cgggctgtat
     2341 gccgcgctcg ccggcagccc gctgatctgg ctggtgatgc tggcgccgct gggcctggcg
     2401 ctgctgctag gcttccgcgt cggccagatg agcccggcgg cggcgcaggc cgcgttctgg
     2461 ggctatgccg gcctgatggg cctgtcgctg gccggcatct tcctggtcta taccggtgac
     2521 agcatcgccc gggtgttctt catcagcgcc ggcaccttcg ctgccaccgc ggcctggggc
     2581 tacaccacgc gcaccgacct cagccggttc ggctcattcc tggtgatggg gctgatcggc
     2641 atcatcctgg ccgggctggt caacctgttc atcggctcca gcgcactgca gttcgccctg
     2701 tcggtgatcg gcgtggcggt gttcaccggg ctgaccgcct gggacaccca gcggctgaag
     2761 gcgatgtacc tggagaatgg cggcgccggc aatctggcga tcctcggcgc gctgatgctg
     2821 tacctcgact tcgtcaacct gtttgtctcg ctgttgcagc tgctcggcgt ccggcgcgac
     2881 tgagcggctg agcaaggcac gagggtgaca tggactggct gaccgatccg caggtctggg
     2941 ccggcctcct gacactgacc gcgctggagg tcgtgctcgg catcgacaac ctcgtcttca
     3001 tcgccgtgct tgtcgcccgc gtgcggcccg ggcaccaggc gctggcccgc cgcttgggtc
     3061 ttggcctggc gctggggacg cggctgctgc tgctggcggg gatcagctgg atcgcccagc
     3121 tgaccgcggc ggtgttcact gttgcaggcc atggtttctc ctggcgcgac ctgatcctga
     3181 tcggcggcgg cctgttcctg gtatggaagg gaaccgccga gatccatgac cgggtggagg
     3241 gcgaatccca tgcacctggc ggcgccgcgc cgcatgccgg cctgtacggc atcgtcttgc
     3301 agatcgccgt gctggacatc gtgttctcgc tggacagcgt gatcaccgcg gttggcatgg
     3361 tcgcccattt gccggtgatg atcgccgcgg tggtgatcgc catgggcgtc atgctgctgg
     3421 cggccgggcc gacgaccgag tttgtcgcgc gccaccccac ggtgaagatg ctggtattct
     3481 ctttcctgct gctgatcggc atgactctgc tggccgacgg cttcggcatg catgtgccga
     3541 agggctacat ctacgcggcg atcggcttct ccgccggggt ggaagtgctg aaccagctcg
     3601 ccggccggcg gcagcgcagg cgcgccggcg cggtatccaa ccatcgcatg catg
//
LOCUS       DASSZH010000271        15539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1046324, whole genome shotgun sequence.
ACCESSION   DASSZH010000271 DASSZH010000000
VERSION     DASSZH010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 15539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15539
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046324"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VFN42_05840"
     CDS             <1..422
                     /locus_tag="VFN42_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003619208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HET8996171.1"
                     /translation="VQPEFLTALRDPNLQAVVVCPSNPFISVEPILALPGVRDALIAC
                     PAPVIAVSPIIGGKAVKGPTAKMMAELGLSPSAGAVAQRYGDLLDGYVVDLADTSEVQ
                     GIRPKVTLAQTLMRTLEDREAVARIVLSAAAKLRRRA"
     gene            419..1063
                     /gene="cofC"
                     /locus_tag="VFN42_05845"
     CDS             419..1063
                     /gene="cofC"
                     /locus_tag="VFN42_05845"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HET8996172.1"
                     /translation="MSDVWAAIPVKGLAGAKQRLSALLDGAQREALAAAMLEDVLTAV
                     ASARVAGILVNTSDPRSAALARRHGAHVVAEDALSGHTGAVVGMSRWLARQGKRAMLA
                     LPGDVPLATAAELDAVIAASREAPCFTIAPAHDELGSNAVLCTPPDAVPLRFGDDSYF
                     PHLIAARRVGLEPVIVRAFGIGLDIDHPADLRALLAIRPRRPIRTIALLEELGV"
     gene            complement(1315..1899)
                     /gene="plsY"
                     /locus_tag="VFN42_05850"
     CDS             complement(1315..1899)
                     /gene="plsY"
                     /locus_tag="VFN42_05850"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HET8996173.1"
                     /translation="MPPPLYILLGYAFGSIPFGLLLTRAAGLGDIRKIGSGNIGATNV
                     LRTGNRKIAAATLLLDAGKGAVAVVLAGLLGGRDAALWAGLGAVLGHNFPVWLAFRGG
                     KGVATAYGVLLAAAWPVGVAAGAVWLVTAKLVRISSAAGLLSFALAPIFAWLLAGPHV
                     ALMALAIAALIYLRHHANIRRLLNGTEPRIGNHR"
     gene            complement(2071..3300)
                     /gene="argJ"
                     /locus_tag="VFN42_05855"
     CDS             complement(2071..3300)
                     /gene="argJ"
                     /locus_tag="VFN42_05855"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019085069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HET8996174.1"
                     /translation="MSAAVSPLAVKLPELPPLPGVRLGATAAGIRYQGRTDLVMMEVP
                     AGSTIAGVFTSNKCPGAPVDWCRAALKGGKARAVVVNAGNANVFTGRAGHNACAATAG
                     AAAELLGCAAKQVFVASTGVIGEVLPHDRLIAALPALHASLREDAWEDAARGIMTTDT
                     FPKASTRIAEIDGAPVRISGIAKGSGMIAPDMATMLCFIFTDAKIPAAAMQALLHKGV
                     DTSFNCTTVDSDTSTSDTVLLVATGQAKHARVPAEGGAMLKDFARALNEVLMDLALQV
                     IRDGEGAQKLVRIDVTGAVNARSAKRIAMAVANSPLVKTAIAGEDANWGRIVMAVGKA
                     GEPADRDKLSIGVGGVWMAKDGGVVPGYDEAPVVAHMKGREVEIAIDLGLGRGKATAW
                     TCDLTHGYIDINGSYRS"
     gene            complement(3329..4291)
                     /locus_tag="VFN42_05860"
     CDS             complement(3329..4291)
                     /locus_tag="VFN42_05860"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET8996175.1"
                     /translation="MPSPFGASARLLAAALLAAGFTSGAALAQTAPPQAAPSKTAPSQ
                     AAPAQATPAQPATAADPVVATVNGQPIHLSDLKEAAAGLPASAQSMPPDQLYPLLLTQ
                     LIDGKALVIEAQKDGLENNPSVQQQIQAAKDRVLESAVLHKAIGAAITEAALKARYEK
                     DIAGKPGAEEVHARHILVPDEATAKKIIAQLDKGADFTALSKQYSKDPGSAQDGGDLG
                     WFKQDEMVPAFSKAAFALKDGQITQTPVHTQFGWHVIQVLGHRKAPAPTFAQAEPELR
                     QKIIKEGVQEAVAKARAQVKVVQYNLDGSTKRATDSAEPPPAKK"
     gene            4574..4649
                     /locus_tag="VFN42_05865"
     tRNA            4574..4649
                     /locus_tag="VFN42_05865"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4607..4609,aa:Ala,seq:cgc)
     gene            4788..6614
                     /gene="ftsH"
                     /locus_tag="VFN42_05870"
     CDS             4788..6614
                     /gene="ftsH"
                     /locus_tag="VFN42_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET8996176.1"
                     /translation="MPGKAPQNWLALLATALFVLGLLILGLFANSVPAPRNIPYSEFQ
                     KYLDTGKIGKVTLSGDTLTGELRDIPAGTNPHFRTQRVPVALAADLAGHGVEFAGVPG
                     AGGLGVLLSWIIPPLIFVGVWLLASGGMSGGGGVLSVGRSKARLVAETEIDVSFADVA
                     GVDEAKAELHEIVDFLKRPAEFTRLGARIPRGVLLVGPPGTGKTLLARAIAGEAGVPF
                     FSTNGAEFVEMFVGVGAARVRDLFERAGEVAPAIIFIDELDALGRARTANPISGQDEK
                     EQTLNQLLAEMDGFDRSVAIVVLAATNRPEILDPALLRAGRFDRQILVDRPDRKGRAD
                     ILRIHLKALKLAPDVKLEDLAALTPGFTGADLANLANEAAVIATRHNAAAIGMADFAS
                     AVERVVAGLEKRSRVLIPKERRVIAYHEMGHALVAMAIPGTDPVQKVSIIPRGIAGLG
                     YTLQRPTEDRFLMNKHELEDKMTVLLAGRAAEMLAGGESYTGAADDLAKATDIARGMV
                     LRFGMDDSVGPVAWDTEQAQFLETPGAFWLPRRFSEATWQAVDHAVHAILDAALARAA
                     AILQANRDVLDRAAAELLARETMTGEEIPRPVPEAAPPRMAA"
     gene            complement(6668..7012)
                     /locus_tag="VFN42_05875"
     CDS             complement(6668..7012)
                     /locus_tag="VFN42_05875"
                     /inference="COORDINATES: protein motif:HMM:NF017690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8996177.1"
                     /translation="MTDTSVVKVQASHAPKGPQGQKYLADGRTLSMRLWEKEAPGKAK
                     PPARRAYETAGYVIAGSAELTLEGQVVRLEPGSSWIIPKGAEHSYRILEEFTAVEATS
                     PPAEVHERDTTP"
     gene            7147..8424
                     /locus_tag="VFN42_05880"
     CDS             7147..8424
                     /locus_tag="VFN42_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HET8996178.1"
                     /translation="MTLWDDSRARAALRSLFDAAVAAADPRKVLARHLPPKPAGRCIV
                     VGGGKSAAVMAAALEDAWPDVALEGTVVTRYGHAVPTRRIEVIEASHPVPDANSEIGA
                     RRLLERVRGLGKDDLVVALMSGGASALLALPAAGLTLADKQAINRALLACGANITEMN
                     CVRKHLSAIKGGRLAAAAAPARVVTLAISDVPGDDPAVIGSGPTVPDASTYAQAREIL
                     ARYGVEPPPAAAARLAAGADETPKPGSLPHAEFRMIATPMMALQAVAETARGLGLAPL
                     VLGDALEGEAREMGTVMAGIARSVRAHGAPLAAPAVLLSGGETTVTLGRGPTGTGGRN
                     TEFLLGLAVALDGAPGIWGMAGDTDGLDGMDEIAGAILAPDTLARVRASGLDPRAMLA
                     GHDSHSLFDAAGDLIRTGPTLTNVNDIRAILIG"
     gene            8437..9870
                     /gene="pyk"
                     /locus_tag="VFN42_05885"
     CDS             8437..9870
                     /gene="pyk"
                     /locus_tag="VFN42_05885"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007001923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET8996179.1"
                     /translation="MAIRPKLRRRRRTKIIATLGPASSSPEMLARLFQAGADVFRLNF
                     SHGTHADHAERFRMIRELEERFQRPIGILADVQGPKLRVGNFSGGRVHLQTGQGFQLD
                     LNPTPGNATRVNLPHPEIIAAASIGTTLLLDDGKLRLRVRRKRDDALETEVVVGGPLS
                     DHKGVNVPDVVLPIPALTEKDREDLAFAVAHGANYIGLSFVQRPEDVAEAKALTGGKA
                     WIMVKLEKPQALDNLDGILALTDAVMVARGDLGVELPPEEVPLAQKRIVRAARQLGKP
                     VVVATQMLESMISAPAPTRAEASDVATAVFDGADAVMLSAETAAGQYPYEAVNMMDRI
                     VARVEQDQGWRAMIDASRPDPEHSAADAIAAAARQVAHTIGAQAIVGFTAFGGTALRV
                     ARERPDAPIIGLTPHEATARRLAVVWGVHAVVLPDVHSMGEAITRASRVATTEGFAQH
                     GDAIVVAAGVPFGQSGSTNALQVAMVK"
     gene            complement(10017..11579)
                     /locus_tag="VFN42_05890"
     CDS             complement(10017..11579)
                     /locus_tag="VFN42_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HET8996180.1"
                     /translation="MTTQPETPRQRRPWRRTAFAAVLLAGTAIGGFTAGHLAFAAPMP
                     NGTTGQATNGQPVNASPVNPQGQNLSGQELPDFVALVDKVKPAVVSITTKLTAQAGQE
                     EAGQAQNGMQQLPFPFSQMFPHPQVRAVEARGSGFIIDANGTVVTNNHVVKGEKSITV
                     TLNDGKTYPATIVGHDPRTDIAVLRIHTNHKLPYIQLGDSSDVKPGQWVVAMGNPFGL
                     GGTVTHGIVSAVSRNIGEGPYDEFIQTDAPINRGNSGGPLFTQQGKVIGMNTAILSPS
                     GGSIGIGFAIPSNMIRQIVSQLEQHGHVIRGFLGVEAQSLTGATAKGLGLAQNSGALV
                     ASVQPNTPAAKAGVQPGDVIESVNGKQIANPHDLALNIAGIEPGQDAHLKILRDGKAQ
                     DMTVRVEQMPSEETASNHQGHSSQQQVGLSLAPLTPGLRNQLDVPEGTSGAVVQGVQP
                     GSPADTAGLQPGDVIVGVNTRPVHSPSQAANEIRHAIHGKDHAVALRVIRNGQAMFVG
                     INAGEAEGNNQG"
     gene            complement(11853..12809)
                     /locus_tag="VFN42_05895"
     CDS             complement(11853..12809)
                     /locus_tag="VFN42_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET8996181.1"
                     /translation="MSLPLGNLPDTRYPDPHIEPLDKRFVYRQGNAAVERIATGFRWA
                     EGPVYFRDGGYLLFSDIPNNRILRWLEEDGHLSVFRAPSNYANGNTRDREGRLVTCEH
                     DTRRVTRTELDGSITVLMDSFNGRKLNAPNDVVVASDGAVWFTDPGYGIDGPYEGHKA
                     EAELPRSVYRLDPRSGDARVVADDFVRPNGLCFSPDERLLYVVDSGITHGGPAHIRVF
                     TVDGANLGKGRVFAEDFAPGMTDGVRTDSDGNLWCSMGWAAPAEDGVRCYAPDGALIG
                     RIHLPEGCANLCFGGRKKDRLFMCASTSVYSLYLNGRGALVP"
     gene            13001..13321
                     /locus_tag="VFN42_05900"
     CDS             13001..13321
                     /locus_tag="VFN42_05900"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HET8996182.1"
                     /translation="MAEAGWVRVAAKSQLAEGQVLGVRLGDKEVAVYHLSGGEFRATD
                     NICTHEYAQLSDGWLEDGCIECPLHAARFDVRTGKALCAPADVDLAVYELKEEGDDLL
                     VKLP"
     gene            13318..14388
                     /locus_tag="VFN42_05905"
     CDS             13318..14388
                     /locus_tag="VFN42_05905"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8996183.1"
                     /translation="MSDPVRPIDCDVHPTVPNMAALLPYLEPVWRDTVVRRGIEDLTT
                     ISYPTVNPVASRADWRDPATNKAATNAALLGEQALAPFDTQLAICNCLYGAQALFSED
                     LGAAFARALNDWIAREWLDRDARLRASIIVPMQNAELAVDEIERCAADPRFVQVLLLV
                     GSELPLGRRQNWPIYAAAQKHGLPVGIHAGSTYRHPVTAVGWPSYWTEDYVNQAPGFQ
                     SQLTSLIAEGVFTKFPDLTVVLMESGVTWLPAYLWRLTKFWKGLRSEIPWVADPPSSV
                     VRDRVRLTLQPFDGPPDPASVMRVMEHIGSDETLLFSTDYPHWQFDALAAIPEGLDAG
                     LAQKIMAENPLRTYPRMRETVT"
     gene            14385..15509
                     /locus_tag="VFN42_05910"
     CDS             14385..15509
                     /locus_tag="VFN42_05910"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8996184.1"
                     /translation="MNVDVLNRTGVQPAASTKLAIADCDVHPRPAGAGIGGISKQLYP
                     YLSQRWREHVETFGIQYRQPWEKGSAYPKGQPQACRIDAWPEGGQPGSDLAFMAKQHL
                     DPNNVALGILNPLTSGQGAMNPDLSAAITHATNEWQVAEWTSRDHRLRASVVVPYEDP
                     DAAVKELELRAGDPNFAQVLFLSRTAEPLGSRKYWPIYETAAAARLPVGIHAFGYGGW
                     PISPGGWGSYYLEEMVGHAQAQQSLLVSLIFEGVFERFPGLKVVLIEGGLAWAPALAW
                     RMDRQWAKLRREIPQVKRPPSEYMRTNVWYTTQPIEEPDPRSQLADVFDWLGWDRVLF
                     ATDYPHWDFDDPAQALPLKMTEQQRRAVFLENAKAVFGVA"
BASE COUNT         2339 a         5142 c         5554 g         2504 t
ORIGIN      
        1 gggtgcagcc ggaattcctg acggccttgc gcgatccgaa cctgcaggcc gtggtggtgt
       61 gcccgtccaa ccccttcatc agcgtggaac cgatcctggc actgcccggg gtgcgtgacg
      121 cgttgatcgc ctgtcccgcg ccggtgatcg cggtgtcgcc gatcatcggc ggcaaggcgg
      181 tgaagggacc gaccgcgaag atgatggcgg agcttgggct gagccccagc gccggcgcgg
      241 tggcgcagcg ttatggcgac ctgctcgacg gctatgtggt cgatctggcg gataccagcg
      301 aggtgcaggg gattcgcccc aaggtgacgc tggcgcagac gctgatgcgc acgctggaag
      361 accgcgaagc ggtggcgcgc atcgtgctga gcgccgcggc gaagctgcgc cgccgcgcat
      421 gagcgatgtg tgggccgcga ttccggtcaa gggactggcc ggggcgaagc agcgcctgag
      481 cgcgctgctg gacggcgcgc agcgcgaggc actggcggcg gcgatgctgg aggacgtgct
      541 gacggcggtg gccagcgcgc gggtggccgg gatcctggtg aacaccagcg atccgcgctc
      601 cgccgcactg gcgcggcgcc atggcgcgca cgtggtggcc gaggatgcgc tcagcgggca
      661 taccggcgcg gtggttggca tgagccgctg gctggcgcgg caaggcaagc gggcgatgtt
      721 ggcgctgccc ggcgacgttc cgctggcgac cgcggcggaa ctggacgcgg tcatcgccgc
      781 atcgcgggag gcaccgtgct tcaccatcgc gccggcgcat gacgagctgg gatccaacgc
      841 ggtgctgtgc acgccgcccg acgcggtgcc gctccgcttt ggcgacgaca gctatttccc
      901 gcatctgata gcggcgcgcc gggtcgggct ggaaccggtg atcgtgcggg ccttcgggat
      961 cgggctggat atcgaccacc cggccgatct gcgcgccctg ctggcgatcc gtccgcgtcg
     1021 cccgatccgg acgatcgcgt tgctggagga attgggggtg taggccggat tcctatgccg
     1081 ccccttcggg tcatgggcgg ccagcctgca actcgcctcc tggcgcggcg tgtcattcgc
     1141 ggtcgaggcc gatgcctacc gcgacggtcg gcggggcgcg ctgcatcagg agccgtaccg
     1201 ggacgatccc gggacagagg acaccagccg agcaacccgg gcatcaccac caccggcgtt
     1261 gtgctcggcg acgacgtgtt cgaccagcgc gcggcgcggt caacgccgcc ttggtcagcg
     1321 gtggttgccg atccgcggct cggtgccgtt cagcaggcgg cggatgttgg cgtggtgccg
     1381 caggtagatc agcgcagcga ttgccagggc catcagcgcg acatgcggtc cggcgagcag
     1441 ccaggcgaag atcggtgcca gcgcgaagga cagcagtccg gcggcggagg agatgcgtac
     1501 cagcttggcc gtgaccagcc agaccgcacc ggcggcaaca ccaaccggcc acgcggcggc
     1561 gagcagcacg ccgtaggcgg tggcgactcc cttgccgccg cggaaggcga gccagaccgg
     1621 gaagttgtgg ccgagcacgg cgcccagccc ggcccacaat gcggcgtcac gaccgcccag
     1681 cagtcccgcc agcacgaccg caaccgcgcc cttgccggca tccagcagca gcgtggcggc
     1741 ggcgatcttg cgattgccgg tacgcagcac gttggtggcg ccgatattgc cggagccgat
     1801 cttccggatg tctcccagcc ccgccgcgcg cgtcagcagc aggccgaacg ggatcgaccc
     1861 gaaggcgtag cccaggagga tgtacagggg agggggcatg gccaactaag ggaagggagg
     1921 cgtggcagaa aatgcaaaac tccccctccc cttgcgggag gggggaaggg ggcgatcgag
     1981 gttttccggg gaatctatga gggccccctc cccccaaccc ccttccgcaa ggggaggggg
     2041 agtcatggcc cccctcccgc tctcagccgc tcagctccgg tacgagccat tgatgtcgat
     2101 gtagccatgc gtcaggtcgc aggtccatgc cgtggccttg ccgcgaccaa ggcccaggtc
     2161 gatggcgatt tccacctccc gtcccttcat atgcgcgacc accggcgctt cgtcgtagcc
     2221 agggaccacg ccgccgtcct tggccatcca cacgccgccg acgccgatgg acagcttgtc
     2281 gcggtcggcg ggctcgccgg ccttgcccac cgccatgacg atgcggcccc aattggcgtc
     2341 ctcgccagcg atcgccgtct tcaccagcgg cgaattcgcc accgccatgg cgatccgctt
     2401 ggcagagcgg gcgttcaccg cgccggtgac gtcgatgcgc accagcttct gtgcaccctc
     2461 gccgtcgcgg atcacctgca gcgcgaggtc catcagcact tcattcaacg cgcgggcaaa
     2521 gtccttcagc atcgcgccgc cttccgccgg cacgcgggcg tgcttcgcct ggccggtggc
     2581 gaccagcagc acggtgtccg acgtggacgt gtccgaatcc accgtggtgc agttgaacga
     2641 ggtatccacg cccttgtgta gcagcgcctg catcgcggcc gcggggatct tcgcatcggt
     2701 gaagatgaag cacagcatcg tcgccatgtc cggcgcgatc atgccgctgc ccttggcgat
     2761 gccgctgatc cgtaccggcg cgccgtcgat ctccgcgatc cgggtggacg ccttggggaa
     2821 ggtgtcggtg gtcatgatgc cgcgcgccgc atcttcccag gcatcctcgc gcagcgaggc
     2881 gtgcagcgcg ggcagcgcgg cgatcagccg gtcgtgcggc agcacctcgc cgatcacgcc
     2941 ggtggaggcg acgaacacct gcttcgcggc gcagccgagc agttcggcgg cggcaccggc
     3001 ggtggcagcg caggcgttat ggccggcgcg gccggtgaac acgttggcat tgccggcatt
     3061 caccaccacc gcccgcgcct tgccgccctt cagcgccgcg cggcaccagt ccaccggcgc
     3121 accagggcat ttgttggagg tgaacacgcc ggcgatggtg ctgccggcgg gaacctccat
     3181 catcaccagg tcggtgcgtc cctggtagcg gatgcccgcc gcggtggcgc ccaggcggac
     3241 gcccggcaac ggcggcagtt ccggcagctt cacggcaagc ggcgagacgg cagcggacat
     3301 cgtttccttc ccccttccca ggtcgggtct atttcttcgc gggcggcggt tcggcgctgt
     3361 cggtggcgcg cttggtcgag ccgtccaggt tgtactgcac cactttcacc tgggcgcggg
     3421 ccttggcaac cgcttcctgc acgccttcct tgatgatctt ctgccgcagc tccggttccg
     3481 cctgcgcgaa ggtcggcgcc ggcgccttgc ggtggccgag cacctggatg acgtgccagc
     3541 cgaactgggt gtgcaccggg gtctgggtga tctggccgtc cttcagcgcg aaggcggcct
     3601 tggaaaaggc gggtaccatc tcgtcctgct tgaaccagcc gagatcgccg ccatcctgcg
     3661 ccgacccggg gtccttgctg tactgcttgg acagcgcggt gaagtcggcg cccttgtcga
     3721 gctgggcgat gatcttcttc gcggttgcct catccggcac caggatgtgg cgggcgtgca
     3781 cttcctcagc gcccggcttg ccggcgatgt ccttctcgta gcgggccttc agcgccgctt
     3841 cggtgatcgc cgcgccgatc gccttgtgca gcacggcgga ttccagcacg cggtccttgg
     3901 cggcctggat ctgctgttgc acggacgggt tgttttccag cccgtccttc tgcgcctcga
     3961 tcaccagcgc cttgccgtcg atgagctggg tcagcagcag cggatagagc tggtcgggcg
     4021 gcatggactg cgcgctggcg ggcaggccgg cggcggcctc cttcaggtcg ctgaggtgga
     4081 tcggctggcc gttcaccgtc gcgaccacgg gatcggcggc ggtggctggc tgcgctggcg
     4141 tggcctgcgc tggcgcggcc tgcgagggcg cggtcttcga tggggcggcc tgcggtggtg
     4201 cggtctgggc cagggcggcg ccggaggtga agccggcggc cagcagggcc gcggcgagca
     4261 agcgggcgga tgccccgaag ggagacggca tgcaaacctc gatatggggt gatggcaggg
     4321 tatggcggaa cgtctggcgg gcgacaaggg cgccggccgg cggccagggg atgcgcgttt
     4381 tccgatagtg ctccggggaa tatagcagag cggaggatgg atttccacgt gagcggcaag
     4441 ggggttttgc cgtgcgggcg gagcggggca gggtggcgcg cgattgcggg gaagcttgct
     4501 tgacttcgcc gaggcggctg agcattgtcc ggccgccgcg agcgaccggt cggtttgctc
     4561 gcatgcgcgg gatggggctg tagctcagtt gggagagcgc ctcgttcgca atgaggaggc
     4621 caggggttcg aatcccctca gctccaccag ttccgcgctt caggctccga caaacacccg
     4681 ctccggtgcg ccagggatgt tgacgtagat caaggcgtct gggccgccgc cgtgtagcct
     4741 ccgctccgca ccacgcattg gccgtggcaa gcgggacgac cggacccatg ccaggcaagg
     4801 caccgcagaa ctggctggcg ctgttggcca cggccctgtt cgtgctgggc ctgttgattc
     4861 tcggcctgtt cgccaacagc gtcccggcgc cgcgcaatat cccctacagc gaattccaga
     4921 aatacctgga taccggcaag atcggcaaag tcacgctgtc cggcgatacg ctcaccggcg
     4981 agttgcgcga cattccggcc ggcaccaatc cgcattttcg tacgcagcgc gtgccggtcg
     5041 ctctcgccgc cgacctcgcc ggccatggcg tggaattcgc cggtgtgccc ggcgccggtg
     5101 gcctgggcgt gctgctgtcc tggatcatcc cgccgctcat ctttgtcggc gtctggctgc
     5161 tcgcctcggg cggcatgtcc ggcggcggtg gtgtgctgtc ggtgggccgc agcaaggcca
     5221 ggctggtggc ggagaccgag atcgatgtca gcttcgccga cgtggccggc gtcgatgaag
     5281 ccaaggcgga actgcacgag atcgtcgact tcctgaaacg ccccgcggaa ttcacccggc
     5341 tcggcgcccg cattccgcgc ggggtgctgc tggtcgggcc gcccggcacc ggcaagacac
     5401 tgctggcgcg cgccatcgcc ggcgaggcgg gggtgccgtt cttctccacc aatggcgccg
     5461 agttcgtcga aatgttcgtc ggcgtcggcg ccgcgcgggt gcgcgacctg ttcgaacgtg
     5521 ccggcgaggt cgcccccgcg atcatcttca tcgatgaact cgatgcgctc ggccgggcac
     5581 gcaccgccaa tccgatctcc ggccaggacg agaaggaaca gacgctcaac cagttgctgg
     5641 cggagatgga cgggttcgac cgctcggtgg cgatcgtcgt gctggcggcg accaaccggc
     5701 cggagatcct ggatccggcg ctgctgcgtg ccggacggtt cgaccggcag atcctggtgg
     5761 accggccgga ccggaagggc cgggcggata tcctgcgcat ccacctcaag gcgctcaaat
     5821 tggcgccgga tgtgaagctg gaggacctcg cagcgctgac ccccggcttc accggcgccg
     5881 atctggccaa cctggccaac gaggcggcgg tgatcgccac gcgccacaat gccgcggcga
     5941 tcggcatggc ggatttcgcc agcgcggtgg agcgcgtggt cgccggcctg gagaagcggt
     6001 cgcgcgtgct gatcccgaag gaacgccggg tgatcgccta tcacgagatg ggccacgccc
     6061 tggtggcgat ggcgattccg ggaaccgatc cggtgcagaa ggtgtcgatc atcccgcgcg
     6121 gcatcgccgg ccttggctat acgctgcagc ggccgaccga ggatcggttc ctgatgaaca
     6181 agcacgagct cgaggacaag atgacggtgc tgctcgccgg ccgagcggcg gagatgctgg
     6241 cgggcgggga gagttatacc ggggccgccg acgatctcgc caaggcgacc gatatcgcgc
     6301 gtggcatggt gttgcgcttc ggcatggatg acagcgttgg gccggtggcc tgggataccg
     6361 agcaggccca gttcctcgaa acgccgggag cgttctggct gccgcgccgg ttcagcgaag
     6421 cgacatggca ggcggtggat catgccgtgc atgccattct ggatgcggcg ctggcgcgcg
     6481 cggcggcgat cctgcaagcc aaccgcgacg tgctggatcg ggccgccgcg gaactgctgg
     6541 cacgggagac gatgaccggc gaggagattc cacgcccggt tcccgaggcc gccccgccgc
     6601 ggatggcggc gtaagagcca gccgccgaca ccgtcgtgac cggtccggcc ggagatgagc
     6661 gccaggctta cggagtcgtg tcgcgctcgt gcacctcggc cggcgggctg gtggcttcga
     6721 cggcggtgaa ttcctccagg atgcggtaac tgtgttccgc gcccttcggg atgatccagg
     6781 aggagccggg ttccaggcgc acgacttggc cttccagcgt cagttcggcc gagccggcaa
     6841 tgacgtagcc ggccgtctca taggcgcgcc gtgcaggcgg cttggccttg ccgggggctt
     6901 ccttctccca cagccgcatc gacagggtgc ggccatcagc gaggtatttc tgcccctgcg
     6961 gccccttcgg tgcgtgcgag gcctgtacct tcaccacgga tgtgtcggtc atgatgtgtt
     7021 gctccgctgc aagccggtcg aggcatcaac ggcgcgggca gggccgggtt cggaccgggc
     7081 gttggcgcct gggcggcgcc tgggccatac tccgcgcccc tggggatacc gaacgcgaga
     7141 ggtcgtatga cgctttggga cgattcccgg gcacgcgccg ccttgcgcag cctgttcgac
     7201 gccgcggttg cggccgccga tccgcgcaag gtgctggccc gtcacctgcc gccgaaaccc
     7261 gccggccgct gcatcgtggt tggcggcggc aagtcggccg cggtgatggc cgcggcgctg
     7321 gaagatgcct ggcccgacgt ggcgctggaa ggcaccgtgg tgacccgcta cggccatgcc
     7381 gtgccgacgc gccggatcga ggtgatcgag gcgtcccatc cggtgcccga cgccaacagc
     7441 gagatcggcg cgcgccgcct gctggagcgg gtgcgcgggc tgggcaagga cgacttggtg
     7501 gtggcgctga tgtccggcgg cgcctcggcc ctgctggcgc tgccggccgc cgggctgacc
     7561 ctagccgaca agcaggcgat caaccgcgcc ctgctggcct gcggggccaa catcaccgag
     7621 atgaactgcg tgcggaagca cctgtccgcc atcaagggcg gccgtctcgc cgcggctgcc
     7681 gcgccggccc gcgtggtgac cctggcgatc agcgatgtgc cgggcgacga cccggcggtg
     7741 atcggctccg gcccgaccgt gccggacgcc tcgacctatg cccaggcgcg cgaaatcctt
     7801 gcccgctatg gggttgaacc gccgccggcc gccgctgccc gccttgccgc cggggccgac
     7861 gagacgccga agcccggcag cctgccgcac gccgaattcc gcatgatcgc caccccgatg
     7921 atggcgctgc aggcggtggc ggagaccgcg cgcgggctgg gcctggcccc gctggtgctg
     7981 ggcgacgcgc tggagggcga ggcgcgcgag atgggcacgg tgatggccgg catcgcccgc
     8041 agcgtccgcg cacacggcgc gccactggcc gcgcccgccg tgctgctgtc gggtggcgag
     8101 accaccgtga ccctcggccg cggcccgacc ggcaccggcg gccgcaatac cgaattcctg
     8161 ctcggccttg ccgtggcgct ggacggcgcg ccgggcatct gggggatggc cggcgatacc
     8221 gacgggttgg acggcatgga cgagatcgcc ggcgccatcc tcgcccccga caccctggcg
     8281 cgcgtgcggg ccagcgggct tgacccgcgt gccatgctgg caggacacga cagccacagc
     8341 ctgttcgatg ccgcaggcga cctgatccgc accggaccga cgctcaccaa cgtcaacgac
     8401 atccgtgcca tcctgatcgg ctgaagaaac cctgccatgg caatccgacc gaagctgcgc
     8461 cgccggcgcc gcacgaagat cattgcgacg ctcggtcccg ccagttcctc acccgagatg
     8521 ctcgccaggc tgttccaggc cggtgccgac gtgttccggc tgaatttctc ccacggcacg
     8581 catgccgatc acgccgagcg gttccgcatg atccgcgaac tggaggagcg gttccagcgg
     8641 ccgatcggca tcctggccga cgtgcagggg cccaagctgc gggtcggcaa tttctccggc
     8701 ggacgggtgc atctgcaaac cgggcagggg ttccaactcg acctgaatcc gacgccgggc
     8761 aacgccacgc gggtgaacct gccgcatccg gagatcatcg ccgcggcctc gatcggcacc
     8821 accctgctgc tggatgacgg caagctgcgc ctgcgggtcc gccgcaagcg cgacgacgcg
     8881 ctggagaccg aagtggtggt cggcggtccg ttgtccgacc acaagggggt caatgtgccc
     8941 gacgtggtgc tgccgatccc ggcgctgacc gagaaggacc gcgaggacct ggccttcgcg
     9001 gtggcgcacg gcgccaacta tatcggcctg tccttcgtgc agcggcccga agacgtggcc
     9061 gaagccaagg cgctgaccgg tggcaaggcc tggatcatgg tgaagctgga aaagccgcag
     9121 gcgctggaca atctggacgg catcctggcg ctgaccgacg cggtgatggt cgcgcgcggc
     9181 gatctgggtg tggaattgcc gcccgaggaa gtgccgctgg cgcagaagcg catcgtccgc
     9241 gccgcccgcc agcttggcaa gccggtggtg gtggcgacgc agatgctgga gagcatgatc
     9301 agcgcacccg cgccgacccg cgccgaggcg tccgacgtcg ccaccgcggt gttcgacggt
     9361 gccgatgcgg tgatgctgtc ggcggaaacc gctgccgggc agtatcccta tgaggcggtc
     9421 aacatgatgg accgcatcgt cgcgcgggtg gagcaggacc aggggtggcg ggcgatgatc
     9481 gatgcctcgc ggccggatcc cgagcactcg gcggcggacg cgatcgccgc ggcggcgcgg
     9541 caggtggcgc acacgatcgg cgcccaggcg atcgtcgggt tcaccgcgtt cggcggtacg
     9601 gcgttgcgcg tggcccggga acggccggat gcgccgatca tcggcctgac gccgcatgaa
     9661 gctacggcac ggcggctggc ggtggtgtgg ggcgtgcacg cggtggtgct gccggacgtg
     9721 cattcgatgg gcgaagcgat cacccgcgcg tcacgcgtgg caacgaccga ggggtttgcc
     9781 cagcacggcg atgccatcgt cgtcgcggcc ggggtaccgt ttggccagtc gggcagcacc
     9841 aatgcgcttc aggtggcgat ggtgaagtaa tagccaaaaa gactcttccc cctccccttg
     9901 cgggaggggg tcagggggag gggtcgttcg tgagttcccg ggaactggcc aaccgacccc
     9961 tccccccagc cccctcccac aaggggaggg ggaggaatca ctgtctgagc gcctgatcag
    10021 ccctggttgt tgccctctgc ctcgccggca ttgatgccga cgaacatcgc ctggccgttg
    10081 cggatcaccc gcagcgcaac cgcgtggtcc ttgccgtgga tggcgtggcg gatttcgttc
    10141 gcggcctgtg acggcgagtg taccggccgg gtgttgacgc cgacgatgac gtcacccggc
    10201 tgcaatccgg cggtatccgc cggggagccc ggctgaacgc cctgcaccac cgcgccgctg
    10261 gtgccttccg gcacgtcgag ctggttgcgc agtcccgggg tcagcggcgc cagcgacaaa
    10321 ccgacctgct gctggctgct gtgtccctgg tggttgctgg ccgtctcttc gcttggcatc
    10381 tgctcgacgc gcacggtcat atcctgcgcc ttgccgtcgc gcaggatctt caggtgcgcg
    10441 tcctggccgg gttcgatgcc ggcgatgttc agcgccaggt catgcgggtt ggcgatctgc
    10501 ttgccgttca ccgattcgat cacgtcgccc ggctgcacgc cggccttggc ggccggggtg
    10561 ttcggctgca ccgacgccac cagcgcgccg gagttctgcg ccaggccgag ccccttggcc
    10621 gtggcgccgg tcagcgactg cgcctcgacg ccgaggaagc cgcggatgac gtggccgtgc
    10681 tgttccaact gactgacgat ctggcggatc atgttggacg ggatggcgaa gccgatgccg
    10741 atcgagccgc cggacggcga caggatcgcg gtgttcatgc cgatcacctt gccctgctgg
    10801 gtgaacagcg gaccgccgga gttgccgcgg ttgatcgggg catccgtctg gatgaactcg
    10861 tcgtacggac cttcgccgat gttgcggctc accgcggaga cgatgccgtg tgtgacggta
    10921 ccacccagcc cgaagggatt gcccatggcc accacccatt gtccgggctt cacgtcgctg
    10981 gaatcgccca gttggatgta tggcagcttg tggttggtgt ggatgcgcag cacggcaatg
    11041 tcggtgcgcg gatcatgccc gacgatggtg gccggatagg tcttgccgtc attcagggtg
    11101 acggtgatgc tcttctcgcc tttgacgaca tggttgttgg tgaccaccgt gccgttggca
    11161 tcgatgatga agccggaacc gcgcgcctca accgcacgaa cctgcggatg cgggaacatc
    11221 tggctgaacg ggaagggcag ttgctgcatg ccgttctgtg cctgcccggc ttcctcctgc
    11281 ccggcctgcg ccgtgagctt ggtggtgatc gagaccaccg ccggcttcac cttgtccaca
    11341 agggcgacaa aatccggcag ctcctgcccg gacaggttct ggccctgcgg gttcaccggc
    11401 gacgcgttga ccggctggcc gttggtggct tgtccggtgg tgccgttcgg catcggggcg
    11461 gcgaacgcca ggtgaccggc ggtgaaaccg ccaatggcgg tgccggccag cagaaccgcg
    11521 gcgaaggcgg tccggcgcca gggtctgcgt tggcgcgggg tctcgggctg ggtggtcatt
    11581 gaacctcctt ggatctcacg gtgggatgtc gcgcgcgcta acgcgaaaca gacgccgggg
    11641 tggcgcggcc catgcgtccc gaaaatgcgg atgcgcagat gaattttcgc ccgatgcggt
    11701 gattaaaccg ctttcatctc gattaaactc ccgtcacatc ccggaacaca tgatattgac
    11761 cggatcgaga cagatccgtt ccgaacggca cgccgcaccg acgcgcgggg ggaggccggg
    11821 acttgattgg ctggcagatt gggactggcg gctcagggca caagagcacc gcggccgttc
    11881 aggtagagcg aatacaccga cgtactcgca cacatgaaca gccggtcctt cttgcgcccg
    11941 ccgaagcaga gattggcgca gccttccggc aggtgaatcc tcccgatcaa cgcgccatcc
    12001 ggcgcgtagc agcgcacgcc gtcttccgcc ggcgccgccc agcccatgct gcaccacaga
    12061 ttgccgtcgc tgtcggtgcg cacgccatcg gtcatgcccg gcgcgaaatc ctcggcgaac
    12121 acccggccct tgccaaggtt tgcgccatcc acggtgaaaa cccggatatg cgcggggccg
    12181 ccgtgggtga tcccgctgtc caccacgtac agcagccgtt cgtccggcga gaagcatagc
    12241 ccgttcggcc gcacgaaatc atccgcgacc acccgggcat ctccgctgcg cggatcgagc
    12301 cggtacacgc tgcgcggcag ctccgcttcc gccttgtgtc cttcataggg tccgtcaatg
    12361 ccgtagccgg gatcggtgaa ccacaccgca ccgtcgctgg ccaccaccac atcgttcggt
    12421 gcgttcagtt tcctgccgtt gaagctgtcc atcagcacgg tgatgctgcc gtccagttcg
    12481 gtgcgggtga cgcggcgtgt gtcgtgctcg caggtgacca gccggccttc gcggtcgcgc
    12541 gtgttgccgt tggcgtagtt ggacggcgcg cgaaagacgg ataagtgccc gtcttcctcc
    12601 agccagcgca ggatgcggtt gttcggaatg tcgctgaaca gcagatagcc gccgtcgcgg
    12661 aaatacaccg gaccttctgc ccagcggaaa ccagtggcga tccgctcgac cgcggcattg
    12721 ccctggcgat agacgaagcg cttgtcgagc ggttcgatat gtgggtccgg atagcgggtg
    12781 tcgggcaggt tgcccaaggg cagggacatc tctcctcctg gcgccgtgat ggatggccgg
    12841 cgtttacgcc ggcatcggca gcgcaaacca tgtcatggcc ggcgaagccc ggccaccagc
    12901 ggcgtgcccg tgcacacggg gcagccatcg ggacgcaagc gggattggcc gatcgtcccg
    12961 gcgctggcat gctgtgggca acaaaaccga ggagagaacg atggcggaag ccggatgggt
    13021 gcgggtcgca gcaaagtctc agttggccga aggccaggtg ctgggcgtgc gcctgggcga
    13081 caaggaagtg gccgtctatc atctgtcggg cggcgagttc cgcgccaccg acaacatctg
    13141 cacgcatgaa tacgcgcagc tttcggatgg ctggctggag gatggctgca tcgaatgtcc
    13201 gttgcacgcc gcccgattcg acgtgcgcac cggcaaggca ctgtgcgccc cggccgatgt
    13261 cgatctggcg gtctatgagc tgaaggagga gggggacgat ctgctggtga agctgccttg
    13321 agcgacccgg tccggccgat cgactgcgat gtccacccga ccgtgccgaa catggccgcg
    13381 ctgctgccgt acctggagcc ggtgtggcgc gacacggtgg tgcgccgcgg catcgaggac
    13441 ctgaccacca tcagctatcc cacggtaaac ccggtcgcct cgcgtgccga ctggcgcgat
    13501 ccggcgacga acaaggcagc gacgaatgcc gcgctgctgg gtgagcaggc gctggcaccg
    13561 ttcgacaccc agttggcgat ctgcaactgc ctctacggcg cgcaggcgct gttcagcgag
    13621 gatctcggcg ccgccttcgc ccgcgcgctg aacgactgga tcgcgcgcga atggctggac
    13681 cgcgatgcgc gcctgcgcgc ttcgattatc gtgccgatgc agaacgcgga actggcggtg
    13741 gacgagatcg agcgctgtgc cgccgatcca cgcttcgtgc aggtgctgct gctggtcggc
    13801 agcgagctcc cgctggggcg gcggcagaac tggccgatct acgccgcggc gcagaagcat
    13861 gggctgccgg tgggtatcca tgccggcagc acctaccgtc acccggtgac cgcggtgggc
    13921 tggccgagct actggaccga ggactatgtg aaccaggcgc cggggtttca gtcccagctc
    13981 acctcgctca tcgccgaagg cgtgttcacc aagttcccgg acctgaccgt ggtgctgatg
    14041 gaatccgggg tgacctggct gccggcctat ctctggcggc tgaccaagtt ctggaagggg
    14101 ctgcgcagcg aaatcccctg ggtcgccgat ccgccttcca gcgtggtgcg cgaccgggtg
    14161 cggctgacgc tgcaaccgtt cgacgggccg cccgacccgg cctcggtgat gcgggtgatg
    14221 gagcatatcg gctccgacga gacgctgctg ttctccaccg actacccgca ctggcagttc
    14281 gacgccctgg cggccatccc ggaggggctg gacgccgggc tcgcgcagaa gatcatggcg
    14341 gagaacccgc tgcgcaccta tcctcggatg agggagaccg tgacatgaac gtggacgtgc
    14401 tgaaccggac cggcgtgcag ccggccgctt ccaccaagct ggcgatcgcc gattgtgacg
    14461 tgcatccgcg cccggccggc gccggcatcg gcgggatcag caagcagctg tatccgtatc
    14521 tgtcgcagcg ctggcgggaa cacgtggaga ccttcggcat ccagtaccgc cagccctggg
    14581 agaagggatc ggcttacccg aaaggacagc cgcaggcctg ccgcattgat gcctggccgg
    14641 agggcggcca gccgggcagc gacctcgcgt tcatggcgaa gcagcatctg gacccgaaca
    14701 atgtcgcgct gggcatcctg aacccgctga ccagcgggca gggggcaatg aaccccgacc
    14761 tctccgccgc catcacccat gccaccaatg agtggcaggt cgccgaatgg acgtcgcggg
    14821 atcaccgtct gcgcgcctcc gtggtggtgc cttacgagga cccggacgcc gcggtgaagg
    14881 aactcgagct gcgtgcgggc gatccgaact tcgcccaggt gctgttcctg tcgcgcaccg
    14941 ccgaaccgct gggcagccgg aaatactggc cgatctatga gaccgccgcc gccgcccggc
    15001 tgccggtcgg catccatgct ttcggctatg gcggctggcc gatctcgccc ggcggctggg
    15061 gctcgtacta cctggaggag atggtcggcc acgcgcaggc gcagcagtcg ttactggtga
    15121 gcctgatctt cgagggggtg ttcgaacgct tccccggcct gaaggtggtg ctgatcgagg
    15181 gcggcctggc gtgggcgccg gcgctggcct ggcgcatgga ccgccagtgg gcgaagctgc
    15241 gccgcgaaat cccgcaggtg aagcgtccgc cgtccgaata catgcggacc aacgtctggt
    15301 acaccaccca gccgatcgaa gaaccagacc cgcggagcca gcttgccgac gtgttcgact
    15361 ggctcggctg ggaccgcgta ctgttcgcca ccgactatcc gcactgggat ttcgatgacc
    15421 cggcgcaggc gctgccgctg aagatgactg agcagcagcg gcgcgcggtg ttcctggaga
    15481 acgccaaggc ggtgttcggc gtggcgtgag cccgcgctgc ggcgcgggtg cgggttacc
//
LOCUS       DASSZH010000272         2008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1540450, whole genome shotgun sequence.
ACCESSION   DASSZH010000272 DASSZH010000000
VERSION     DASSZH010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540450"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /locus_tag="VFN42_05915"
     CDS             complement(<1..1362)
                     /locus_tag="VFN42_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET8996185.1"
                     /translation="MAENPHASGVGLSTLFIRRPIATSLLMVALLLFGIFGYRTLPVA
                     ALPNVEAPSLQVTTQYPGASPSTMARLVTTPLERQFGQISGLTMMSSNSSQGLSVITL
                     QFVQSRDIANAEQDVQAAINAARGTLPATLPYPPVYNAVNPADAPILTLALTSDTLPV
                     REVTNYADSILAQKLSQISGVGLVSVEGNIKPAVRIQVNPAALANLGLTLEDVRSAIT
                     QANVNAPKGSLNGSTQSFTIGANDQLPDAAAYRDLIIRYVAGAPVRLSEVATVINGVE
                     NDQVAAWADGRPAVLLDIRRQPSANIVQTVAAIRAELPGLRAVLPAGVDLRVFADRTV
                     TIRASVRDVEFTLLLAIVLVVMVIFVFLRRLWATVIPSVAVPLSLVGTFGVMAFAGFS
                     LDNLSLMALTVATGFVVDDAIVMIENIVRYIEKGRQPREAAEVGAREIGFTIVSLTVS
                     LIAV"
     gene            complement(1355..>2008)
                     /locus_tag="VFN42_05920"
     CDS             complement(1355..>2008)
                     /locus_tag="VFN42_05920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET8996186.1"
                     /translation="PIKGVAGLRLVDVGNVVGPGTSGGIVVLTQIHPISVLFNLPQQN
                     LDAVRAGMAKGPLQVAALDRGDNKVIASGVLRVVSNQIDTSTSTFQLKAEFANPNDTL
                     WPGQFVNVRLQLGTLDNALVVPVQAVQRGPDGDYVFVVQPGNTVKMQPVTTGGEAGAS
                     RTVIRSGLTPGQRVVTAGQFRLKQDSKVIPLAPGEVPPPPKVPAQTDRQSGGRARRG"
BASE COUNT          311 a          672 c          706 g          319 t
ORIGIN      
        1 caccgcgatc agcgacaccg tcagcgagac gatggtgaag ccgatttcgc gcgcgccgac
       61 ctcggcggcc tcgcgcggct gccggccctt ttcgatgtag cgcacgatgt tctcgatcat
      121 cacgatggca tcgtcgacca cgaagccggt ggccacggtc agcgccatca gcgagaggtt
      181 gtccagcgag aagcccgcga aggccatcac tccgaaggtg cctaccagcg acagcggcac
      241 cgccaccgag gggatcaccg tggcccacag tcggcgcagg aacacgaaga tcaccatcac
      301 caccagcacg atggccagca gcagggtgaa ctcgacgtcg cgcaccgagg cgcggatggt
      361 cacggtgcga tcggcgaaca cccgcaggtc gaccccggcc ggcagcaccg cgcgcaggcc
      421 gggcagctcg gcgcggatgg cggccacggt ctgcacgatg ttggcgctgg gctggcggcg
      481 gatgtccagc agcaccgccg gcctgccgtc ggcccaggcg gcaacctggt cgttctcgac
      541 gccgttgatc accgtggcca cctcggacag ccgcaccggt gcgccggcga cgtagcggat
      601 gatcaggtcg cggtaggcgg cggcgtcggg caattgatcg ttggcgccga tggtgaagct
      661 ctgcgtgctg ccgttgaggc tgcccttggg cgcgttgacg ttggcctggg tgatggcgct
      721 gcgcacgtcc tccagggtca ggccgaggtt ggccaacgcc gccgggttga cctggatgcg
      781 caccgccggc ttgatgttgc cctcgacact gaccagtccc acgcctgaga tctgcgagag
      841 cttctgcgcc aggatgctgt cggcgtagtt ggtgacctcg cgcaccggca gggtgtcgga
      901 ggtcagcgcc agggtgagga tgggcgcgtc ggccgggttc accgcgttgt acaccggcgg
      961 gtagggcagt gtcgccggca gcgtgccgcg cgcggcgttg atcgcggcct gcacgtcctg
     1021 ctcggcgttg gcgatgtcgc ggctctgcac gaactgcagg gtgatcacag acagtccctg
     1081 cgagctgttg gagctcatca tggtcaagcc ggagatctgc ccgaactgcc gctccagcgg
     1141 cgtggtcacc agccgcgcca tggtcgaggg actggcgccg ggatactgcg tggtcacctg
     1201 caggctgggc gcctcgacgt tgggcagcgc cgccaccggt agcgtgcggt agccgaaaat
     1261 gccgaacagc agcagcgcca ccatcagcag tgaggtggcg atcgggcggc ggatgaacag
     1321 cgtcgacagc ccgacgcctg acgcatgcgg gttttcagcc acggcgggcg cgtccgccgg
     1381 attggcgatc ggtctgcgcc ggcaccttgg gcggcggcgg cacctcgccg ggcgccagcg
     1441 ggatcacctt gctgtcctgc ttcagccgga attgccccgc ggtgaccacg cgctggccgg
     1501 gcgtcagccc gctgcgaatc acggtgcggc tggcaccggc ttcgccaccg gtagtcaccg
     1561 gctgcatctt caccgtgttg ccgggttgca ccacgaatac ataatcgccg tccgggccgc
     1621 gctgcaccgc ctgcaccggt accaccagcg cgttgtccag cgtacccagt tgcaggcgca
     1681 cgttgacgaa ctgaccgggc cacagagtgt cgttcgggtt ggcgaactcg gccttgagct
     1741 ggaacgtgga ggtgctggtg tcgatctggt tactgaccac gcgcaggacg ccgctggcga
     1801 tcaccttgtt gtcgccgcga tccagcgcgg ccacctgcag cgggcccttg gccatgcccg
     1861 cgcgcacggc atcgaggttc tgctgcggca ggttgaacag cacactgatc ggatggatct
     1921 gcgtgagtac cacgatgccg ccgctggtgc cggggccgac cacgttgccc acatccacca
     1981 gacgcagtcc ggccacgccc ttgatcgg
//
LOCUS       DASSZH010000273         3354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1216655, whole genome shotgun sequence.
ACCESSION   DASSZH010000273 DASSZH010000000
VERSION     DASSZH010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216655"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2083
                     /locus_tag="VFN42_05925"
     CDS             <1..2083
                     /locus_tag="VFN42_05925"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HET8996187.1"
                     /translation="AWTVTSENVLPAGRKTRALLAVVALASPRPALRGRLAELLWSRR
                     PEEQARASLRQEIHRLLETLSPAGTEVLQVTRDHITLKPGVAWVDVDEVMRATTSQPA
                     SLSLLDGDLLEDLDGIDPTFDAWLTTERERLRDRARSIAEALLREQVEPEAAIPAAQR
                     LLQIDRAHEGAWRALMRAYAARGERGMAIQAYDRCRAVLADLLDTAPSQETQKLLAEI
                     RGPYGSRIPVRSQNAAAPEAVPPAPQPAAASAPEPQPAAEPVHEPAAAEEPRATTRPA
                     RGGIHVGVMPLQLVGTTEEEAHLAPGLAEEITTALARFRWMFVVASSSLARFAAENRD
                     ESAIRRTFGVDFLLDGTIQRVKNRLRITMRLLDLRAGNQVVWARRFDRQTSDLLSLQD
                     EISAEVVAQIDPEILLIEARRSASQAQGDASAYDLVLRAIPLIGRLERGTFMQAGQSL
                     ARAVEQEPDYGSAHAWYAYWNTFLISQGWADDPAAAVERAGQLAERAIVLDPFDARAL
                     SIAGHVRAFLHRRLRESLSLHERALSLNPNLAMAWALSGAAYAYLGDTEEAERRITRY
                     KKLSPLDPHAFFYDTAFITVALLKRDYESAVIAGRAVSEMNPSFSAAYQPYLAALGHL
                     GRESEAKMVRERLLGLEPAFTIGRFLQNCPFERESDSAFYAEGLRLAGVAEGEREAES
                     AGAHATTQFGK"
     gene            complement(2380..>3354)
                     /gene="dnaN"
                     /locus_tag="VFN42_05930"
     CDS             complement(2380..>3354)
                     /gene="dnaN"
                     /locus_tag="VFN42_05930"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019085734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HET8996188.1"
                     /translation="IAVVEDVPASTTRNGSCTAPAATMYEIVRKLPDGAEVELDHPGG
                     DAQLALRAGRYSTSLVVLPTEDFPSMTAGALPHKFSLSAQQLRAMIDRTRFAISTEET
                     RYYLNGIYLHVAETEGTRVLRAVATDGHRLARVEEPLPEGAGSMPGVIIPRKTVNELR
                     KLLDEVSGNVEIGLSDTRIQFHVDNILLTSKLIDGTFPEYERVIPRGNDKVLKVDKKD
                     FADAVGRVAAISSERSRPVKLSLARDLLVLSANSPELGNASEELDADHVKYEAGPLEI
                     GFQARYLNDITDQITGEVEFRFSDGSAPTVVCDAGDASALYVLMPMRV"
BASE COUNT          562 a         1124 c         1125 g          543 t
ORIGIN      
        1 agcctggacg gtcacgagtg agaacgtact gccggcgggc cgaaaaaccc gggccctgct
       61 ggcggtggtg gcactcgcct ccccccgccc tgccctgcgc ggccggcttg ccgaactgct
      121 ttggagccgc cgtccggagg agcaggcacg cgcgtcgctg cggcaggaaa tccatcgcct
      181 gctggagacg ctctcgcccg ccggcaccga ggtcctgcag gtcacgcgcg accacatcac
      241 gctgaagccg ggcgtcgcct gggtggatgt ggacgaggtg atgcgcgcca ccaccagcca
      301 gccggcctca ctatcgctgc tggacggcga cctgctggag gatctggacg gcatcgatcc
      361 caccttcgac gcctggctga cgacggaacg cgagcgcctg cgtgaccgcg cccgcagcat
      421 cgccgaggcg ctgctacgcg agcaggtgga accggaagcc gccattcccg ccgcgcagcg
      481 actgctgcag atcgaccgcg cgcacgaagg agcttggcgc gcactgatgc gggcatacgc
      541 cgcacgcggc gagcgtggca tggcgatcca ggcctatgac cgctgcagag cggtactcgc
      601 ggatctgctg gatacggcgc cgtcgcaaga gacgcagaaa ctgctggcgg aaattcgcgg
      661 cccctacggc agccgcatcc cggtgcgcag ccagaacgca gcggcgccgg aggctgtacc
      721 tcccgcaccg caacccgctg ctgcttcggc gcccgaaccg caacctgccg cagagccggt
      781 gcacgaaccg gctgccgcgg aggaaccgcg cgccaccacg cgaccggcgc gcggcggcat
      841 ccatgtcggc gtgatgccgc tgcagcttgt cggcaccacg gaagaagaag cgcatctcgc
      901 cccgggcctt gcggaggaga tcaccaccgc gctcgcccgc ttccgctgga tgttcgtcgt
      961 cgcctccagt tcgctggccc gcttcgcggc ggagaatcgc gacgaaagcg cgatccgccg
     1021 gacattcggc gtcgacttcc tgctggatgg cacgatccag cgggtgaaga accggctgcg
     1081 catcaccatg cgcctgctcg atctgcgtgc cggcaaccag gtggtgtggg cgcgccggtt
     1141 cgaccggcag accagcgacc tgttgtcgct gcaggacgag atctccgcgg aagtggtggc
     1201 gcagatcgat cccgaaatcc tgctgatcga agcgcgccgc agcgccagcc aggcacaggg
     1261 cgatgccagc gcctatgacc ttgtgctgcg cgccatcccg ctgatcggcc ggctggagcg
     1321 cgggaccttc atgcaagccg gccaatctct ggcccgcgcg gtggagcagg aaccggacta
     1381 cggatcggcg catgcctggt acgcctattg gaacaccttc ctgatcagcc agggctgggc
     1441 cgatgatccg gcggcggcgg tggaacgcgc cgggcaattg gccgagcggg cgatcgtgct
     1501 cgatcccttc gatgcgcgcg ccttgagcat cgccggccat gtacgtgcgt tcctgcatcg
     1561 tcgcctgcgc gaatcactgt cgctgcacga gcgggcgctg tcgctgaatc caaatctggc
     1621 gatggcgtgg gcgctgtccg gcgccgctta tgcgtatctc ggcgatacgg aagaagcaga
     1681 gcggcggatc acgcggtaca agaagctgtc gccgctggat ccccacgcgt tcttctacga
     1741 cacggcgttc atcaccgtcg cgctgctgaa gcgcgactac gaatcggcgg tgatcgccgg
     1801 gcgtgccgtc agtgagatga acccgtcctt ctccgctgcg taccagccgt atctcgcggc
     1861 gctgggtcat ctgggccgcg agtcggaagc gaagatggtg cgcgaacggc tgctgggact
     1921 ggaaccggca ttcacgatcg ggcggttcct gcaaaactgc ccgttcgagc gcgaatccga
     1981 cagcgccttc tatgccgagg ggctgcgcct ggcgggcgtg gcggaaggcg agcgcgaagc
     2041 cgaatccgcc ggcgcgcacg cgacaacgca gttcggcaag tagcaccggc agaggagagg
     2101 gcggcggtac agcgtcactg ccggcgaggc gtcatggcct ctgcttcgcg cgcccgccac
     2161 gacgttatat gcgcgtcagg tgtgtcactc tttacgcggg ttggtatcat accaactgcc
     2221 gagcaaatga cacaccgggc gcagtatcac gtcgtggcgg acgaaggccc cgaaacccac
     2281 gactttgctg ggcgcaattg aggaaagtcg tcgatgccgg cctttgccgg catgacggaa
     2341 ttcggcgtgc cgaagagtcg agctcctcgc gggttggtat tacacccgca tcggcatcag
     2401 cacatacagc gcactcgcat cgccggcgtc gcacaccacg gtcggggcgg agccgtccga
     2461 gaagcggaac tccacctccc cggtgatctg gtcggtgatg tcgttcaggt agcgcgcctg
     2521 gaaaccgatc tccagcgggc cggcctcata cttcacgtga tcggcgtcca actcctcgct
     2581 ggcattcccc agttccgggc tgttcgctga cagcaccagc aaatcgcgcg ccagagacaa
     2641 cttcaccggg cgggaccgtt cggaggaaat cgccgcgacg cggcccaccg cgtcggcgaa
     2701 atccttcttg tccaccttca gcaccttgtc gttgccgcgc gggatcaccc gctcgtattc
     2761 cgggaaagtg ccgtcgatca gtttgctggt cagcagaatg ttgtccacgt ggaactggat
     2821 gcgcgtatcg gacaggccga tctccacatt gccgctgacc tcgtcgagca acttgcgcaa
     2881 ctcgttcacc gtcttgcgcg ggatgatcac gccgggcatc gaaccggcgc cttccggcag
     2941 cggctcctcg acccgcgcca gccgatgacc gtcggtggcg accgcgcgca gcacgcgggt
     3001 gccctcggtt tcggccacgt gcaggtagat gccgttcagg taatagcggg tctcttccgt
     3061 gctgatggcg aagcgggtgc ggtcgatcat cgcgcgtagc tgctgcgccg acaggctgaa
     3121 cttatgcggt agcgcgccgg cagtcatcga ggggaaatct tcggtgggca gcaccaccag
     3181 gctggtggaa tagcgaccgg cccgcaatgc gagctgggcg tcgccgccgg gatggtccag
     3241 ctccacttcc gcgccgtccg gcaatttgcg aacgatttcg tacatggtcg cggcaggcgc
     3301 ggtgcaggag ccgttgcgcg tggtgctggc gggtacgtcc tccaccaccg cgat
//
LOCUS       DASSZH010000274         4083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1676993, whole genome shotgun sequence.
ACCESSION   DASSZH010000274 DASSZH010000000
VERSION     DASSZH010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676993"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..1478
                     /locus_tag="VFN42_05935"
     CDS             276..1478
                     /locus_tag="VFN42_05935"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HET8996189.1"
                     /translation="MEQHAAFAGLKVIDLTGGVAGPSCAMMFAQHGADVIKVETPHNG
                     GDWSRILGRRYQDHSAFSLYGTLGKRSLALDLKQPEAKDILWRLLRGADVFMEGFRPG
                     TIQRYGFGYQEVSEREPQIIYYSISGFGQTGPLAARPAMDPVLQAMIGIVTENRGEHD
                     NHPHRIAISLIDMFTGLLGFQAIVTALYTMREQTEKRGRFIEASLMQGGAMLSIIRLI
                     ANYLERGTTTRTSMPNGVFNTADGQLNITMVRPTDWVPFCDAIGHPALAEHPHYATAQ
                     VRGENLDELYTVVRPIIAAVPTAWLAERLTQRNIMNGKVNTYEEFLQEAQVDATGIIS
                     WLTQPGVPEKVPMPNIPGLPPFQDGTRRAHAPRLGEHSVEVLREHGYGEAEIARLLDK
                     KVISEPQR"
     gene            1873..3414
                     /locus_tag="VFN42_05940"
     CDS             1873..3414
                     /locus_tag="VFN42_05940"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HET8996190.1"
                     /translation="MLLEKTRQLEATLAGMSDGIMMVDAELRLLAWNDKFPQFTGVPA
                     DMLYVGATMQDVLRAQALAGEFGAVDVEAEVVRRLDLLRRISSIGVLERQRPNGQVIE
                     LRRNPLPGGGFVTLYTDVTARRAAEERMREAQKMAAIGRLTAGVAHDFNNVLTSIMGS
                     AELLERRLGDDPAAARKLAVILQGAQRGADLVQQLLAFSRKQPLVPIAVEPNNVVRGM
                     GELLRTTLGGAVRVETRLNARYPALVDPVQIEHVILNLAINARDAMPDGGVLTIATED
                     VVLDAPLEEAGLARGDYVMVSVADTGCGMPPDVQRQALEPFFTTKPPGRGSGLGLSQV
                     YGVANQSGGGVQIDSALGRGTRISVFLPRAATAPVQPPVPAIGPAKTELARSQGTVLL
                     VEDEPAVRETIAGMLTSLGFTALAAEDAAAALRLVESGRGFDLLLADLVMPGMDGVQL
                     AGLVRARRPSLPVVLITGYSDDRRVSGERWVLSKPFLASRLGETLGMALRQRDAAGTT
                     ECQEG"
     gene            3466..>4083
                     /locus_tag="VFN42_05945"
     CDS             3466..>4083
                     /locus_tag="VFN42_05945"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET8996191.1"
                     /translation="MSTEPKRGLARNTANYGDRDFALYLRRSFAKSMGYSQAMLDKPV
                     VGIVDTGSDYNNCHRTVPDLVEAVKRGVLAAGGLPLAFPTVSLCEPSLTPTSMHYRNL
                     AALDTEAMLTAQPMDAAVLIGGCDKTVPAQLMAAVSADVPAVQLVTGPMLATPFQGER
                     LSACTDCRRYWAAYRAGRVTAERIAQIEGRLATTAGTCGVVGTAST"
BASE COUNT          687 a         1357 c         1394 g          645 t
ORIGIN      
        1 tgcacagagg ttttgcccgg ttgagggcgg ctatgcggac cgtctggccg tcggtgtccg
       61 ctgctgggag gggagctatt ggtggggagc cgaatgttgc tggcccgcaa gacgggattg
      121 gcggcctcgt gccagaagca gttgctcgcg gggaacctct cgggcaacgg cggaaggcaa
      181 accgaccgag caacacgccc gcttaccagc gggtatagcc acccgcgcgc cacccgcctt
      241 aacatcgccg gcagaaacca agggcaggag acaccatgga gcagcacgcc gcctttgccg
      301 gcctgaaagt catcgacctg accggcggcg ttgccggccc gtcctgcgcc atgatgttcg
      361 cgcagcacgg tgccgacgtc atcaaggtgg aaaccccgca taacggcggc gactggtcac
      421 gcatcctcgg ccgccgctac caggatcatt ccgccttctc gctctacggc actctgggca
      481 agcgcagcct ggcgctggac ctgaagcagc cggaagccaa ggacatcctc tggcgcctgt
      541 tgcgcggggc cgacgtgttc atggagggat tccgcccagg caccatccag cgctacggct
      601 tcggctacca ggaagtcagt gaacgcgagc cgcagatcat ctactactcg atctccggct
      661 tcgggcagac cggtccgctg gcggcgcgcc cggcgatgga cccggtgctg caggcgatga
      721 tcggcatcgt caccgagaac cgcggcgagc acgacaacca tccccaccgc atcgccatct
      781 cgctgatcga catgttcacc ggcctgctcg gcttccaggc gatcgtcacc gcgctgtaca
      841 cgatgcgcga gcagacggag aagcgcggac gcttcatcga ggccagcctg atgcagggcg
      901 gcgcaatgct gtcgatcatc cggctgatcg ccaactacct ggaacgcggc accaccacgc
      961 gcaccagcat gcccaacggg gtgttcaaca ccgcggacgg gcagctcaac atcaccatgg
     1021 tacggccgac cgactgggtg ccgttctgcg atgcgatcgg ccatccggcg ctggccgaac
     1081 atccgcatta cgccaccgcc caggtacggg gtgagaacct ggacgagctg tacaccgtcg
     1141 tgcgtccgat catcgccgcg gtgccgaccg cctggctggc ggagcggctc acccagcgca
     1201 acatcatgaa cggcaaggtg aacacctatg aggaattcct gcaggaagcg caggtggacg
     1261 ccaccgggat catttcgtgg ctgacccagc cgggtgtgcc ggaaaaggtg cccatgccga
     1321 acatccccgg cctgccgccg ttccaggacg gtaccaggcg ggcgcatgcg ccgcggctgg
     1381 gggagcactc ggtggaggtg ctgcgggagc acggctacgg cgaggcggag attgcccgcc
     1441 tgctggacaa gaaggtcatc agcgaaccgc agcgctgagg gcctgaccgg gcgttaaggc
     1501 ctcggctgcg aacgtcagca tcaagtcatg ggcgcgccaa ccgtacgtca atcctgaaag
     1561 aatttcgatc cgcatccgtc acccgtaatg ttcccgatga cgattccatg acggttctgg
     1621 caggaattgg atgaaccgag gggagatcag catatttgat tgactgcggc gctcggacgc
     1681 gatcacatgc gcgagattgc ttcaggggtc ctttcctcgt ctgcccgccc cggctgaaag
     1741 agtgctgcaa tgcgggaaat catcccgacc tgttcggcct ttcccccgtt tggtcgttgg
     1801 attggcggtg gcgcctcgtc accacgcccg cgttcggcgg cggccgccaa agccccgcca
     1861 gacgaccgcg caatgctgct ggagaagacc cggcagctgg aagccaccct ggccggcatg
     1921 agcgacggca tcatgatggt cgatgcggag ctccgcctgc tggcctggaa cgacaagttc
     1981 ccgcaattca ccggcgtgcc ggccgatatg ctgtatgtcg gggcgaccat gcaggatgtg
     2041 ttgcgcgccc aggccctggc cggcgaattc ggcgcggtgg atgtcgaagc cgaggtcgtc
     2101 cgtcgcctgg acctgctgcg ccgcatcagc agcatcgggg tactggagcg tcagcgtccc
     2161 aacggccagg tgatcgaact ccggcgcaat ccgctgccgg gcggcggatt tgtcacgctg
     2221 tacaccgacg tgaccgcgcg gcgcgccgcc gaggagcgca tgcgtgaagc acagaagatg
     2281 gcggcgatcg ggcgcctcac cgccggtgtg gcgcacgatt tcaacaacgt gctcacctcc
     2341 atcatgggaa gcgccgagct gttggaacgg cggctgggtg atgatccggc cgcggcgcgc
     2401 aagctggcgg tgatcctgca gggggcccag cgcggcgcgg atctggtgca gcaactgctg
     2461 gcgttctcgc gcaagcagcc gctggtgccg atcgccgtgg agccgaacaa cgtcgtgcgc
     2521 ggcatgggcg aattgctgcg caccacactg ggtggcgccg tccgcgtgga gacccggctg
     2581 aacgcccgat atcccgcgct cgtcgatccg gtgcagatcg agcacgtcat cctcaatctt
     2641 gcgatcaacg cgcgcgatgc catgcccgat ggcggcgtgc tgaccatcgc caccgaagac
     2701 gtggtgcttg atgcgccctt ggaggaagcc ggtctcgccc gcggcgatta cgtcatggtg
     2761 tcggtggccg ataccggctg cggcatgccg cccgacgtgc agcggcaggc gcttgaaccc
     2821 ttcttcacca ccaagccacc ggggcgtggt tccggcctgg gactgagcca ggtctacggc
     2881 gtggccaacc aatccggcgg cggggtgcag atcgacagcg cccttggtcg cggtacgagg
     2941 atcagcgtct ttctgccgcg tgcggcgacg gcgccggtgc agccgccggt tcccgccatc
     3001 ggcccggcga agaccgagct ggcgcgcagc cagggcacgg tgctgctggt ggaagacgaa
     3061 cccgcggtgc gggagacgat tgccggcatg ctgaccagcc tcggcttcac cgctctggcc
     3121 gcagaggatg ctgcggcggc gctgcggctg gtggagtccg ggcgcggctt cgacctgttg
     3181 ctggccgacc tggtgatgcc tgggatggac ggcgtgcagt tggcggggct cgtgcgggca
     3241 cgccgcccgt cgctgcccgt ggtgttgatc accggttata gcgatgatcg ccgcgtcagt
     3301 ggcgagcgct gggtgctgag caagcccttc ctggcatccc gtctgggaga gacgctggga
     3361 atggcgctcc ggcagcgtga cgccgccggc accacggaat gccaggaagg ctgatcgccg
     3421 gcaccggtca tgacagggcg cgccgacgcg cctaacatgc cgcgcatgag caccgaaccg
     3481 aagcgcggcc tggcccgcaa caccgccaat tacggcgacc gtgatttcgc gctgtacctg
     3541 cgccggtcct tcgccaaatc catgggctac tcgcaggcca tgctggacaa gccggtggtc
     3601 ggcatcgtcg ataccggcag tgactacaac aactgccacc gcaccgtgcc ggacctggtg
     3661 gaagcggtga agcgcggcgt cctggcggcc ggcgggctgc cgctggcctt tcccactgtg
     3721 tcgctgtgcg agccgtcgct gacgccgact tccatgcact accgcaacct ggctgcgctg
     3781 gacaccgagg cgatgctgac cgcgcagccg atggatgccg cggtgctgat cggcggctgc
     3841 gacaagaccg tgccggcgca actgatggcc gccgtttcgg ccgacgtccc cgcggtgcaa
     3901 ctggtgaccg gcccgatgct ggcgacgccg ttccagggcg agcggctgtc cgcctgcacc
     3961 gattgccgcc gctactgggc cgcctaccgc gccggccgcg tcaccgcgga gcggatcgcc
     4021 cagatcgagg gacgattggc caccacggcg gggacatgcg gcgtggtggg caccgcctcc
     4081 acc
//
LOCUS       DASSZH010000275         3502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1318969, whole genome shotgun sequence.
ACCESSION   DASSZH010000275 DASSZH010000000
VERSION     DASSZH010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318969"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VFN42_05950"
     CDS             <1..246
                     /locus_tag="VFN42_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin"
                     /protein_id="HET8996192.1"
                     /translation="SMVIDVYIWLAYRLHALRRDVEVGWPALHAQFGAGYMRLRDFRR
                     DFIAALELALAAYPDAHVSLGERGVVLRPSRPAVARA"
     gene            complement(380..1000)
                     /locus_tag="VFN42_05955"
     CDS             complement(380..1000)
                     /locus_tag="VFN42_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HET8996193.1"
                     /translation="MPDTEPTQEDSVVPRLVEGRSCGSCNVCCVALTIDDPALQKVQG
                     YRCRHLKPDQGCGIYETRPHTCRTFFCGWRQLKWVRESLRPDTSGVLVELAGEIAPDG
                     TKQLGVSIFLLTRAALKAEGLAETVAAAVAARIPVFLTIPGPPGHTSARAKINAHLEP
                     AVAAKDKAAVLAFLDQAQRKGRRGKSRRITFSGQSGQPGVIQLDGG"
     gene            complement(1080..2195)
                     /locus_tag="VFN42_05960"
     CDS             complement(1080..2195)
                     /locus_tag="VFN42_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET8996194.1"
                     /translation="MTVPEENVTLQTGLFGPLPLPAHRSDAHELEDLSRRAALYATRA
                     RGDGTRRAYRSAWKHFTAWCEALGRSPLPADPDTLAMYVVRRADDGLAVSSLRVALAA
                     IRTAHLLAGLSLDLRHPRLAMVLEGITRSKGVRPRRQASPAVPGVLRLMLATRPPADT
                     AMGARDRAMLLLGFGAALRRSELVGLAIGDIEPVPGRGLRLTVRRSKTDQHGAGQQVA
                     VWANPNEPGFCPAVALQAWLVFRHQAPDLAGAASDAERPLFCGLTKAGRPIGANLSDK
                     AVVRLVKQAAAAAGLDPARYSGHSLRAGLATAAGDEGAGLAELMRQTRHRSTEVALGY
                     LRPADLWRNNVTERLFRPAPEGEGASPRDPAPVSQAG"
     gene            complement(2192..2548)
                     /locus_tag="VFN42_05965"
     CDS             complement(2192..2548)
                     /locus_tag="VFN42_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996195.1"
                     /translation="MTSGRAGVFDDDDDDALDVTSFAPKPAAPPGISSDQVRVVSEGA
                     QFRSRDPAPPAEKRPPRRYRTGRNVQLNVKARAEAIETFYAIADAQGWVLGEAFEQAV
                     AALERELAGRSDAAAT"
     gene            complement(2545..3240)
                     /locus_tag="VFN42_05970"
     CDS             complement(2545..3240)
                     /locus_tag="VFN42_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011251564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HET8996196.1"
                     /translation="MPTIVFASPKGGVGKSTSAVVLAAEFARKGAAVTLIDADPNRPV
                     SQWARRPGRPPNITVITSASEATIIDEIEGAATRTAFVIVDLEGTASMTVAYAISRAD
                     LVIVPTQGSQLDAAEAAKAIKLIRQQERAFGRRIPYAVLFTRTSSAIRPRTLQHIQDE
                     FAQHGVSGFATQMHEREAFRAIFSFGGTLESLDPKQVSNRAAAIANARAFAAEVVGML
                     RTPAEAEPAEAAQ"
BASE COUNT          531 a         1158 c         1241 g          572 t
ORIGIN      
        1 tccatggtga tcgacgtgta tatctggctg gcctaccggc tgcacgcgct gcgccgcgac
       61 gtggaggtcg gctggccggc gctgcacgcg cagttcggcg ccggctacat gcggctgcgc
      121 gatttccgcc gggacttcat cgccgcgctg gaactggcgc tggccgccta cccggatgcg
      181 catgtctcgt tgggcgagcg cggcgtcgtg ctgcggccgt cccgtcccgc cgtggcccgc
      241 gcctagcgcg agcaaagtcc aaacccggac cggctggcgc gagtaaagtc caaagccatg
      301 ctccggctgc tgcgcaacgc ctcgcgcgag tggagtccaa aggcagcggc gtcagcctgg
      361 cagcgagtag agtccaacct caaccgccgt ccagctgaat gacacccggc tgtccgctct
      421 ggccgctgaa ggtgatgcga cgggacttgc cacgccggcc cttgcgctgc gcctgatcca
      481 ggaacgccag taccgctgct ttgtctttgg cggcgaccgc gggctccaga tgcgcattga
      541 tcttcgcccg ggcggaggtg tggccgggag gacccgggat ggtcaggaat accgggatcc
      601 tggcggccac ggcggcggca acggtctccg ccagcccctc cgccttcagc gccgcgcggg
      661 tgagcaggaa gatggagacg cccagttgtt tcgtgccgtc gggcgcgatc tccccggcga
      721 gctccaccag cacgccggat gtatcggggc gcagcgactc ccgaacccat ttcaactgcc
      781 gccagccgca gaagaaggtc cggcaggtgt gcggacgggt ttcgtagatg ccgcatccct
      841 ggtccggttt cagatggcgg cagcgatagc cctggacttt ctgcagcgcg ggatcgtcga
      901 tcgtcagtgc cacgcagcag acgttgcagg agccgcagct acggccctcg accaggcggg
      961 ggacgacgct gtcctcttga gtgggctcgg tgtcgggcat ccggattcgt ccccgcaggg
     1021 cctccacagg gggctcttgg tccgatcata cagccagccg cggcgaaaag gcggatcgtt
     1081 cagccggctt gcgacaccgg tgcgggatcg cgcggtgaag ccccctcccc ttccggcgcc
     1141 ggccggaaca gccgctcggt gacgttgttg cgccagagat cggcaggccg cagatagccc
     1201 agcgccactt cggtcgagcg gtgccgggtc tggcgcatca gttcggccag ccccgcgcct
     1261 tcgtcgccgg cagcggtagc caggccggcg cgcagcgaat gcccggaata ccgcgccgga
     1321 tcgagcccgg cggcggccgc cgcctgcttc accagccgca ccacggcctt gtccgacagg
     1381 tttgctccga tcggccggcc cgccttggtc aggccgcaga acagcgggcg ttcggcgtcc
     1441 gaggcggcgc cggccagatc cggtgcctgg tggcggaaca ccagccaggc ttgcagcgcg
     1501 acggctgggc agaagccggg ctcattcgga ttagcccaca ccgcgacttg ctgaccggca
     1561 ccatgctggt cggtcttgga gcggcggacg gtcagccgca ggccgcggcc aggcaccggc
     1621 tcgatgtcgc cgatcgccag cccgaccaac tccgagcggc gcagcgcggc gccaaagccc
     1681 agcagcagca tggcgcggtc gcgcgcaccc atggctgtat cggccggggg acgagtggcg
     1741 agcatcagcc gcagcacgcc gggtacggcc gggctggcct gccggcgggg gcgaacgccc
     1801 ttcgaccggg tgatgccctc cagcaccatg gccaggcgcg gatgacgcag gtcgagcgac
     1861 aggccggcga gcagatgcgc ggtgcggatc gccgccaggg cgacgcgcag cgaactgacc
     1921 gccagtccgt catcggcgcg gcgcaccacg tacatcgcca gcgtgtcggg atcggccggc
     1981 agcggactgc ggcccagcgc ctcgcaccag gcggtgaaat gcttccaggc agatcggtag
     2041 gctcggcggg tgccgtcgcc gcgggcgcgg gtcgcgtaga gcgcggctcg ccgggatagg
     2101 tcctccagct catgggcatc ggaccgatgc gcgggcaggg gcagtgggcc gaacaggccg
     2161 gtctgcaacg tgacgttttc ctcgggtacg gtcatgtcgc tgcggcgtca ctccggccag
     2221 cgagttcgcg ttcgagcgcg gcaacggcct gttcgaaggc ctcgcccagc acccagccct
     2281 gggcgtcggc aatggcgtag aaggtctcga tcgcctcggc gcgcgctttg acgttcagtt
     2341 gcacgttgcg gccggtgcgg tagcgcctgg gtgggcgctt ttcggctggc ggggccgggt
     2401 cgcggctgcg gaactgggca ccttcggaga ccacgcgcac ctggtcggag gaaatcccgg
     2461 gcggcgcggc cggcttgggc gcgaagctgg tgacatccaa cgcgtcatca tcatcgtcgt
     2521 cgaatacccc tgccctgccg gaggtcattg tgccgcctcc gccggctcgg cctcggccgg
     2581 cgtgcgcagc atgccgacca cttcggcggc gaaggcccgg gcattggcga tcgcggcagc
     2641 gcggttgctg acctgcttgg gatctaggct ctccagcgtg ccgccgaagg agaagatggc
     2701 acggaacgcc tcgcgttcgt gcatctgcgt ggcgaagccg ctgacgccgt gctgggcgaa
     2761 ttcatcctgg atgtgctgca aagtgcgcgg gcggatggcc gagctggtgc gggtgaacag
     2821 cacggcataa ggaatccggc ggccgaaggc gcgttcctgc tgccggatca gcttgatcgc
     2881 cttggcggct tccgcggcat ccagttgtga gccctgcgtg gggacgatca ccagatccgc
     2941 ccggctgatt gcgtaagcga cggtcatcga ggcggtgcct tccagatcga cgatgacgaa
     3001 ggcggtgcgg gtggccgcgc cctcgatctc gtcgatgatg gtggcttccg aggcgctggt
     3061 gatgacggtg atgttgggcg gacggccggg ccggcgggcc cactgggaca cagggcggtt
     3121 gggatcggcg tcgatcaggg tgaccgcggc gcctttgcgc gcgaattcgg cggcgagcac
     3181 gacggcggag gtggattttc caacgccacc ctttggcgag gcgaagacga tggtcggcat
     3241 ggggtctcct tcactggaag gaagctatcc ggtttctgac cgatatcgga atgccttcag
     3301 tcggtcaacg gaaagctatc tagtactatt tattttacct ggtactaaag agctatcccc
     3361 cccccccccc gcgaccgtct taatttagta ccagatagct ttctgatatc atttcctatg
     3421 cggcgaaggc ccggaaactg ccccttccgg tgacctcttt gacaacagaa cccttctcca
     3481 aggttcccag gatccggttt gc
//
LOCUS       DASSZH010000276         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_57663, whole genome shotgun sequence.
ACCESSION   DASSZH010000276 DASSZH010000000
VERSION     DASSZH010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57663"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VFN42_05975"
     CDS             complement(<1..401)
                     /locus_tag="VFN42_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET8996197.1"
                     /translation="MPQRFWNSAWILLILGNLFWAGNIVLGRGVAGEVPPIALAYWRW
                     TGAFLIAFGFAWPHLKQDFPTLLRHWKLMLLLSATGIACYNTMSYIGLNSTTALNVLL
                     LQSAMPLVIVVWAFALFGDRPTARQSAGVLIS"
     gene            596..1024
                     /locus_tag="VFN42_05980"
     CDS             596..1024
                     /locus_tag="VFN42_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996198.1"
                     /translation="MSKWLAAVSLLLGAAMLQGCVYDPNAGTYAPAAGYGYGGYPGYG
                     GYGYPAYGYPASSYPAYGPSYGGSVTFGGMWGGGERRDDWDHRDQGARREEHRPDRAG
                     RPVNLGAGFHPTPPQGGYHPPAASAGRGDNDRDKDRGEHH"
     gene            complement(1100..1303)
                     /locus_tag="VFN42_05985"
     CDS             complement(1100..1303)
                     /locus_tag="VFN42_05985"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HET8996199.1"
                     /translation="MGIVRNIDVTSQSEISFDDACRTAVTEAAKTLRGIMSFRLTDAE
                     CVVEHDRVIAYRVTGKIVFTVER"
     gene            complement(1463..>2001)
                     /gene="pgi"
                     /locus_tag="VFN42_05990"
     CDS             complement(1463..>2001)
                     /gene="pgi"
                     /locus_tag="VFN42_05990"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003541.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HET8996200.1"
                     /translation="PIVFGEPGTNAQHSFMQLVHQGTRMVPVDFILAAEPDHDRPDAH
                     RALAANALAQAEALLRGRSEPEIRAEMAAKGASPQEIDAVAPHRVCSGERPSNFILFR
                     RLDAFSLGRLIALYEHKVAVQGCLWGIDSFDQWGVELGKQLAGGILPELEPGVVPGAH
                     DSSTSGLMRRFRGLRGEA"
BASE COUNT          341 a          643 c          708 g          309 t
ORIGIN      
        1 gagatcagca caccggcgga ttgccgcgcc gtcggccggt cgccgaacaa ggcgaaggcc
       61 cacaccacga tcaccagcgg catggccgat tgcagcagca gcacgttcaa cgccgtggtg
      121 ctgttcaggc cgatatagga catggtgttg tagcaggcga tgccggtggc ggagagcagc
      181 agcatcagtt tccagtggcg gagcagggtc ggaaagtcct gcttcaggtg aggccaggcg
      241 aagccgaagg cgatcaggaa ggcgccggtc cagcgccagt aggcgagcgc gatcggcggc
      301 acctcgcccg cgaccccgcg gccgagcacg atgttgccgg cccagaacag gttgccgagg
      361 atgagcaaga tccaggcgga attccagaaa cgctggggca aggtcggggt ccattgggtg
      421 gcgaagaggc gtggaacgga cagaaatacc ggcaaccgcg gcgcggtgtc actggcaacg
      481 ggcggtcggc caaggtggcc agggcgctca actcatgccc ttgcgcgcgc tgcacggttc
      541 taaccggggc ggagagcggt tagcctgcgc gctgaacaga acccggagat gtggcgtgag
      601 caaatggctg gcggcggtat cactgcttct tggcgcggcc atgctgcagg gctgcgtgta
      661 tgatccgaat gccggcacct acgcgccggc ggcggggtac ggctacggcg gctatccagg
      721 atacgggggc tatggctatc cggcctacgg ctatccggcc tccagctatc ctgcctatgg
      781 cccgtcctat ggcgggtcgg tgacgttcgg cgggatgtgg ggcggcggcg agcggcggga
      841 tgattgggat catcgcgacc agggcgcgcg tcgcgaggag catcggccgg atcgtgccgg
      901 tcgtcccgtc aaccttggtg cgggattcca ccccacgcct ccgcaaggcg gctaccaccc
      961 tccggcagcg tccgctggcc gcggcgacaa cgaccgtgac aaggaccgcg gcgagcatca
     1021 ttgatctcgc tgcggcgggc gtcacggctg gcgacgcgcg gttcgccagc cgtggcggga
     1081 ccggctgggc gatcggaacc tagcgctcga cggtaaacac gatcttgccg gtcacccgat
     1141 aggcgatgac gcggtcatgc tccaccacgc actcggcgtc ggtgaggcgg aacgacatga
     1201 tgccgcgcag ggtcttggcg gcttcggtca ccgccgtgcg gcaggcatcg tcgaagctga
     1261 tttcggactg cgacgtcaca tcgatgttgc ggacgattcc catcccacgc tcctggcaaa
     1321 aagaaacggc acgacttgtt cgcccacgaa cgagctgccg ccgaccgagg tcccggcgcg
     1381 tgcaagcttg ccccgatcac gcagccaggc aagcgaatac tggcgcggtg cccatcagcc
     1441 gcccgtgagt ccggtggtat cgctatgcct ccccgcgcag gccgcggaac cgtctcatca
     1501 gcccggaggt ggagctgtca tgcgcgcctg gcactacgcc tggctccagt tccggcagga
     1561 tgccgccggc aagctgcttg ccgagttcca cgccccactg gtcgaagctg tcgatgcccc
     1621 acaggcagcc ctgcacggcc actttgtgtt catacagcgc gatcagccgg cccaggctga
     1681 atgcatccag gcggcggaac agaatgaagt tggacggccg ctcgccggaa cagacgcggt
     1741 gcggcgccac agcgtcgatc tcctgggggg aggcgccctt cgctgccatc tcggcgcgga
     1801 tttccggctc cgaccggccg cgcagcagtg cctcggcctg cgccaaggca ttggcggcca
     1861 gcgcgcggtg cgcgtccggc cggtcatggt cgggttcggc ggccaggatg aagtccaccg
     1921 gcaccatgcg tgtgccctgg tgcaccagct gcatgaagct gtgctgtgcg ttggtgcctg
     1981 gttcgccgaa cacgatcggg c
//
LOCUS       DASSZH010000277         2916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_687981, whole genome shotgun sequence.
ACCESSION   DASSZH010000277 DASSZH010000000
VERSION     DASSZH010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687981"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VFN42_05995"
     CDS             <1..632
                     /locus_tag="VFN42_05995"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HET8996201.1"
                     /translation="IAAAQRGEHAMLFAFDEGRGTLEARAHSLGLPLEELLEAGLIRH
                     QQIDPAELSPGEFDSIVRHAVEHDNARVIIIDSLNGYLNAMPDERFLILQMHELLSYL
                     GQQGVVTLLILAQHGLVGPMETPLDMSYLSDAVLMLRYFEVEGTVRRALSVVKKRSGH
                     HEHTIREFRLTAEGISVGPPLTAFSGIFAGSPRYTGGSSPARPPADDET"
     gene            619..2301
                     /locus_tag="VFN42_06000"
     CDS             619..2301
                     /locus_tag="VFN42_06000"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8996202.1"
                     /translation="MTKPDADSGRVLILMPIGRDGPTSLDLLRRIGLAGHICTDLGAV
                     VAGIQAGAATVLIAEEGLFGQDLAGLTHWVAQQAPWSDLPFIVLTSHHEHPEVAAWRQ
                     RLVPALRNVTMIERPMQAITLTSSVQAAVRARLRQYEVRTLLEAQARAAQQLEALVKA
                     RTHELEEANAALRQQMAERAQIEEALRQAQKMDAIGNLSGGIAHDFNNLLQGVAGSLD
                     LIRRRPNDHQRVRRWAEAGLQAAERGARLTAQLLSFSRAQRVEAKPVIVAELLDGIRD
                     LLSRTLGPMIRVTMELDSDRMPVLSDPTQLEMAVLNLAINARDAMPGGGDLHLSSRVR
                     RILHDPELQPGEYVELSVTDTGEGMPADVVSRAFDPFYTTKAIGKGTGLGLSQVYGIA
                     RQAGGTARIESRPGQGTTVRMLLRRTELPLAADAATTPPDADTGTRAATVLVVDDDAD
                     VRRFMADALDTLGYHTLEAENGEAGLQMIDGAIPQLVVVDFAMPGMNGAEFARSVWAR
                     HPDMPIVFASGYAETAAIEEVAGPNSLVLRKPFRITELQGIATEALRSRVRA"
     gene            2408..>2916
                     /locus_tag="VFN42_06005"
     CDS             2408..>2916
                     /locus_tag="VFN42_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA cytosine-C5-methylase"
                     /protein_id="HET8996203.1"
                     /translation="MTPAARIAAAIELLDLVLIASRPADAVANDFFRARRFIGSGDRR
                     AVSGRVWQVLRTRRRLGWWLDRQGAPVTARLLAGASLLLEGWSLPGLTEAYAGGRFGP
                     PPLGQTELQALRPLEGQPLDHPDMPEAVRLELPDWLLPPLRTRFGAELAAELAALGKA
                     APLDLRTNLL"
BASE COUNT          466 a          998 c         1022 g          430 t
ORIGIN      
        1 cgatcgccgc ggcgcaacgc ggcgaacacg ccatgctgtt cgccttcgac gagggccgcg
       61 gcacgctgga agcacgcgcg cacagcctgg gattgccgct ggaggaactg ctcgaagccg
      121 ggctgatccg gcatcagcag atcgatcccg ccgagctttc gcccggtgag ttcgacagca
      181 tcgtccggca cgcggtggag catgacaacg cccgggtcat catcatcgac agcctgaacg
      241 gctacctgaa tgcgatgccg gatgaacgtt tcctgatcct gcagatgcac gaactgctca
      301 gctacctggg acagcagggc gtggtgacgc tgctgatcct ggcgcagcac ggcctggtgg
      361 ggccgatgga aacgccgctg gacatgagct atctcagcga tgccgtgctg atgctgcgct
      421 atttcgaagt ggagggaacg gtgcggcgcg cgctgtcggt ggtgaagaag cgcagtggcc
      481 atcacgaaca cacgatccgg gaattccggc tcaccgcgga aggcatcagc gtggggccgc
      541 cgctgaccgc gttcagcggc atctttgccg gtagcccacg ctatacgggc ggaagctcac
      601 cggcccgccc acccgccgat gacgaaacct gacgcggata gcggccgcgt cctgatcctg
      661 atgccgatcg ggcgagacgg tcccacctcg ctggacctgc tgcggcggat cgggctggca
      721 ggacacatct gcacggacct gggcgccgtc gtcgccggca tccaggccgg cgccgcgacg
      781 gtgctgatcg ccgaggaagg cctgttcggc caggacctcg ccggcctgac gcactgggtg
      841 gcgcaacagg caccctggtc cgatctgccg ttcatcgtgc tcaccagcca tcatgaacac
      901 ccggaggttg ctgcgtggcg gcagcggctg gtgccggcgc tgcgcaacgt caccatgatc
      961 gagcggccga tgcaggcgat cacgttgacc agcagcgtgc aggccgcggt gcgtgcccgg
     1021 ttgcggcaat acgaggttcg cacgctgctg gaggcgcagg cgcgggccgc ccagcaattg
     1081 gaggcgctgg tcaaggcccg gacccacgaa ctggaggagg cgaacgccgc cctgcggcag
     1141 caaatggcgg aacgggcgca gatcgaggag gcgttgcgcc aggcgcagaa aatggatgcg
     1201 atcggcaatc tgtccggcgg catcgctcac gatttcaaca acctgttgca gggcgtcgcc
     1261 ggcagcctgg atctgatccg ccgccggccg aacgaccatc agcgcgtgcg gcgctgggcg
     1321 gaagccgggc tgcaggcggc ggagcgcggc gccaggctga ccgcgcagct actctccttc
     1381 tcccgcgcac aacgggtgga ggcgaagccg gtcatcgtcg cggaactgct ggatggcatc
     1441 cgggatctgc tgtcccgtac tctggggccg atgatccggg tgacgatgga actggattcc
     1501 gatcgtatgc cggtgctgtc cgatcccacg caactggaga tggcggtgct gaacctggcc
     1561 atcaatgcgc gcgacgcgat gccagggggc ggcgatctgc acctgtccag ccgcgtcagg
     1621 cgcatcctgc acgacccgga actgcagccg ggcgagtatg tggaattgag cgtgaccgat
     1681 accggtgagg gaatgcccgc cgatgtcgtg tcccgcgcct tcgatccgtt ctacaccacg
     1741 aaggccatcg gcaagggcac cgggctgggg ttgagccaag tatacggcat cgcccgccag
     1801 gccggcggca ccgcccgcat cgaaagccgg ccggggcaag gcaccacggt gcgcatgctg
     1861 ctgcgccgca ccgaactgcc gctcgctgcc gacgcagcca ccacgccgcc cgatgcggac
     1921 actggaacgc gcgcggcgac cgtgctggtg gtggatgacg acgcggatgt ccgccgcttc
     1981 atggccgatg cgctggatac gctgggctat cacaccctgg aagcggagaa cggcgaagcc
     2041 ggactgcaga tgatcgacgg cgccattccc cagcttgtgg tggtggactt cgccatgccg
     2101 ggcatgaatg gcgccgagtt cgcccggtcg gtctgggcac ggcacccgga catgccgatc
     2161 gtgttcgcca gcggctatgc ggaaacggcg gcgatcgagg aggtggccgg cccgaacagc
     2221 ctggtgctgc gcaagccgtt ccgcatcacc gagctgcagg gcatcgccac cgaggcgctg
     2281 cggagccggg tccgcgcctg actccgctgc tggcgcgcga tcgggcgggt gtggccggcg
     2341 cgaaacctgc cgcctatatc agcgccatgc cctcgcccac atccggtcgc agccggtctg
     2401 agactgcgtg acccccgccg cccgcatcgc cgccgccatc gaattgctgg acctggtgct
     2461 catcgccagc cgcccggccg atgccgtggc caacgacttc ttccgcgccc gccgattcat
     2521 cggctctggc gaccgtcgcg ccgtatccgg ccgggtctgg caggtgctgc gcacccgccg
     2581 ccggctcggc tggtggctgg accgccaggg agccccggtc accgcgcgcc tgctggccgg
     2641 cgccagcctg ctgctggaag gttggtccct gcccggcctc accgaggcct atgccggcgg
     2701 ccggttcggc ccgccgccgc tcggccagac cgaactccag gcgctgcgcc cgctggaggg
     2761 ccagccgctg gaccatccgg acatgcccga agcggtccgg ctggagctgc ccgactggct
     2821 gctgccgccg ctgcgcaccc ggttcggcgc cgaactggcg gcagagctgg cggcgctggg
     2881 gaaagccgca ccgctggacc tgcggaccaa cctgct
//
LOCUS       DASSZH010000278         3704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1114509, whole genome shotgun sequence.
ACCESSION   DASSZH010000278 DASSZH010000000
VERSION     DASSZH010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114509"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFN42_06010"
     CDS             <1..447
                     /locus_tag="VFN42_06010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET8996204.1"
                     /translation="LPNGSAYDQSGKITFIDNQVDPATGTITIWADFANPQRLLIPGA
                     YVTVEVRRAKPEEKPMVPVAAAQTDQKGSYVLLVGPGDKVKQQPVTLGRQVAQNFIVD
                     KGLSGGEQVIVQGMQKVRPGEVVKPVAQPPANQTASQGAAAGQQGG"
     gene            451..3591
                     /locus_tag="VFN42_06015"
     CDS             451..3591
                     /locus_tag="VFN42_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012569002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HET8996205.1"
                     /translation="MLSTLFIRRPIFALVISIVIVLAGGVALWAMPVSQYPDITPPQV
                     TVSASYPGASAETLASTVGQPIEEQVNGVPGMLYMQSTSSSSGTYSLQVTFAIGTNPD
                     IDQVNVQNRAQLAIARLPTEVQQEGLTVRSASSNFVLAINLYSPTRKFDQVTISNYAY
                     MHLEQEIARIPGVGDTRIFGERQYAMRVWLDPVRMTALGLTATDVVNAIRSQNIQVAA
                     GQIGQPPTGAGQQRQLTVLAPGRLSSPQQFDNVIVRTTPNGGVVRIKDIGRVQLAAQQ
                     YSSGSALDGSPSATLAVYQAPGANALALAKTIEARMQALSKQFPTGLHYAIVYNATNF
                     VRANIDDILYTLAITLALVVGVVFVFLQDWRATLIPTIAIPVSLIGVFAVLYAVGYSA
                     NTVDLFAIVLAVTLVVDDAIVVVENVTRHLEQEPGISRADATDRAMREITGPVIASTL
                     VLVAVFGPVGFIAGVTGALYRQFAVTISISVLISAVNALTLSPALCALILRPPRPAHF
                     FLFRWFNRGFDASRNGYGRTVRVLSRRLLLGLGGVALAFGVTYLIFVRTPTEFLPSED
                     QGYFFTAVQLPNGASLARTQKVVDQVGQMLLHTPGVAHAIELTGFSLVSGAQSASAGS
                     VIGIMKPWGERGAAETIPAVIARLAPRFNAIPAAQIVSFNPPAIPGISTTGGINFVLE
                     GRNGQSYQQLAAVARGLIFAGNQNPNLKAVFTTFSANVPQIMVHVDTARAALMGVTPA
                     AVYQALQANLGGQFVNDFNYQNFTFQVIVQDEPQFRSTVSDIGTLHVKSSTGAMVPLQ
                     SLVSITTVEGADAINRYDLYPSVLINGSGAPGKSSGQAIAAMQQVAAQHLPAGYGYDW
                     TAMSFQELQAAGQEAYAFAFAVVFSYLFLAALYESWTLPLSVVLPVVFALLGGLFAIL
                     VRGMALDVYAQIGLVLLIGLAAKNAVLIVEFAKYRWERGDVSLEQAGEEGARTRFRPV
                     MMTAMAFIIGVIPLVLASGAGAGARESIGTTVFGGMIFASLIGILFVPPLFVAFERLS
                     ERIVRLFRGRHEHPAE"
BASE COUNT          582 a         1222 c         1270 g          630 t
ORIGIN      
        1 ctgccgaacg gcagcgcgta cgaccagtcg ggcaagatca ccttcatcga caaccaggtg
       61 gatcccgcca ccggcacgat caccatctgg gcggacttcg ccaatccgca gcgcctgctg
      121 atcccgggcg cctacgtcac cgtggaggtg cggcgcgcca agccggagga gaagccgatg
      181 gtgccggtcg ccgcggcgca gaccgaccag aagggcagct acgtgctgct ggtggggccc
      241 ggcgacaagg tgaagcagca gccggtgacg ctggggcggc aggttgcgca gaacttcatc
      301 gtggacaagg ggctgagcgg cggcgagcag gtgatcgtcc agggcatgca gaaggtgcgc
      361 ccgggcgagg tggtgaaacc ggtggcgcag ccgccggcga accagacagc gtcacagggc
      421 gccgccgccg ggcagcaggg cggctgaggc gtgctgtcca cgcttttcat ccggcgcccg
      481 atcttcgcgc tggtgatctc catcgtgatc gtgctggccg gcggggtggc gctgtgggcg
      541 atgccggtgt cgcagtatcc ggacatcacc ccgccgcagg tgacggtctc cgccagctat
      601 cccggtgcca gcgccgagac gctggccagc accgtcggcc agccgatcga ggagcaggtg
      661 aacggcgtgc cgggcatgct gtacatgcag agcaccagct ccagctccgg cacctattcc
      721 ctgcaggtga ccttcgcgat cggcaccaat cccgacatcg accaggtgaa cgtgcagaac
      781 cgggcgcaac tcgccatcgc ccggctgccg accgaggtgc agcaggaagg cctgacggtg
      841 cggtccgcct ccagcaactt cgtgctggcg atcaacctgt attcgccgac caggaagttc
      901 gaccaggtca ctatctccaa ctacgcgtac atgcacctgg agcaggagat cgcccgcatt
      961 cccggtgtgg gcgatacccg catcttcggc gagcgccagt atgccatgag ggtgtggctg
     1021 gacccggtgc ggatgaccgc gctggggctg accgcgacgg atgtggtgaa cgccatccgg
     1081 tcgcagaaca tccaggtggc ggcgggccag atcggccagc cgccaaccgg tgccggccag
     1141 cagcggcaac tgaccgtgct ggcgccaggg cggctgagct cgccgcagca attcgacaac
     1201 gtgattgtgc gcaccactcc gaatggcggc gtggtgcgga tcaaggatat cggccgggtg
     1261 cagctcgcgg cgcagcaata cagctcgggg tccgcgctgg atggttcgcc ctcggcgacg
     1321 ctggcggtgt accaggcgcc gggagccaat gcgctggcgc tggcgaagac gatcgaagcc
     1381 cgcatgcagg cgctgtcgaa gcaattcccc accgggctgc actacgccat cgtctacaac
     1441 gccaccaact tcgtgcgcgc caatatcgac gacatcctgt acacgctggc gatcacgctg
     1501 gcgctggtgg tgggcgtggt gttcgtcttc ctgcaggact ggcgcgccac gctgatcccc
     1561 accatcgcca tcccggtgtc gctgatcggc gtgttcgcgg tgctgtacgc ggtgggctat
     1621 tccgccaaca cggtcgatct gttcgccatc gtgctagcgg tgaccctggt ggtggatgac
     1681 gccatcgtgg tggtggagaa cgtcacccgg catctggagc aggagccagg gatttcccgc
     1741 gccgatgcca cggatcgggc gatgcgggag atcaccgggc cggtgatcgc cagcacgctg
     1801 gtgctggtgg cggtgttcgg cccggtcggc ttcatcgccg gcgtcaccgg cgcgctgtac
     1861 cgccagttcg cggtgaccat ctcgatttcc gtgctgattt ccgcggtcaa cgcgctgacg
     1921 ctgagtccgg cgctttgcgc gctgatcctg cgcccgccgc gcccggcgca cttcttcctg
     1981 ttccgctggt tcaaccgcgg cttcgatgcc agccggaacg gctacggccg aaccgtgcgg
     2041 gtgctgtccc gccgcctgct gctcggcctg ggcggcgtgg ccctggcctt cggcgtcacc
     2101 tacctgatct tcgtccgcac gccgaccgag ttcctgccga gcgaggacca gggctacttc
     2161 ttcaccgccg tgcagttgcc gaatggcgcc tcgctggcgc gcacgcagaa ggtggtggac
     2221 caggtcgggc agatgctgct gcacacgccc ggcgtggcgc atgcaatcga gctgaccggg
     2281 ttcagcctgg tcagcggcgc gcaatccgcc agcgccggca gcgtgatcgg catcatgaag
     2341 ccctggggcg aacgcggggc tgcggaaacc atccccgcgg tgatcgccag actggcgccg
     2401 cgcttcaacg ccattcccgc ggcacagatc gtcagcttca atccgccggc gattcctggg
     2461 atcagcacga cgggcggcat caacttcgta ctggagggac gcaacggcca gtcctaccag
     2521 cagcttgccg cggtggcgcg cggactgatc ttcgccggca accagaaccc caatctgaag
     2581 gccgtgttca ccaccttcag tgccaacgtg ccgcagatca tggtgcatgt ggataccgcg
     2641 cgggcggcgc tgatgggggt gactccggcc gcggtgtacc aggcgctgca ggccaatctg
     2701 ggcggccagt tcgtcaacga cttcaactac cagaacttca ccttccaggt gatcgtgcag
     2761 gacgagccgc agttccgcag caccgtcagc gatatcggca cgctgcacgt gaagagcagc
     2821 accggcgcga tggtgccgct gcaaagcctg gtgtcgatca ccaccgtcga gggcgccgac
     2881 gcgatcaacc gctatgacct gtatccctcg gtgctgatca acggctccgg cgcaccgggc
     2941 aagagttccg gccaggcgat cgcggcgatg cagcaggtgg cggcgcagca tttgccggcg
     3001 ggctacggct acgactggac cgccatgtcg ttccaggaac tgcaagcggc cgggcaggag
     3061 gcctatgcct tcgccttcgc ggtggtgttc tcctacctgt tcctggcagc gctgtacgaa
     3121 agctggacgc tgccgctctc ggtcgtgctt cccgtggtgt tcgccctgct cggcgggctg
     3181 ttcgccatcc tggtgcgcgg catggcgttg gacgtctatg cgcagatcgg cctggtgctg
     3241 ctgatcggcc tggcggcgaa gaacgcggtg ctgatcgtgg agttcgcgaa gtaccgctgg
     3301 gagcggggcg atgtcagcct ggagcaggcg ggcgaggagg gcgcgcgcac gcggttccgc
     3361 ccggtgatga tgacggccat ggccttcatc atcggcgtga tcccgctggt gctggccagt
     3421 ggtgccgggg ccggcgcgcg cgaatcgatc ggcaccaccg tgttcggcgg catgatcttc
     3481 gcttcgctga tcggcatcct gtttgtgccg ccgctgttcg tcgcgttcga acggctgtcg
     3541 gagcgcatcg tgcgactgtt ccgcggcagg cacgaacatc cggccgaata gcgcccgcac
     3601 gctcccgtgt catggccggc gcaggccggc caccgatgtc tttgctcgtc gcatttacgc
     3661 ggatggggac atgccgccat cgcagcccgt ttttccgtcc aact
//
LOCUS       DASSZH010000279         2125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_142865, whole genome shotgun sequence.
ACCESSION   DASSZH010000279 DASSZH010000000
VERSION     DASSZH010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142865"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /gene="cobO"
                     /locus_tag="VFN42_06020"
     CDS             <1..485
                     /gene="cobO"
                     /locus_tag="VFN42_06020"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003629252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HET8996206.1"
                     /translation="KGKSTAAYGLVLRMLGHGGRVGVVQFIKGTWQTGERTAFAAFGD
                     RVRWHSMGEGFTWETQDRARDIAAAERAWSVAAGMLREPALDLVVLDELNIALRYDYL
                     QLATVLADLAARPPRQHVVVTGRNAKPELIEAADLVTEMGAVKHHFAAGVRAQKGIEY
                     "
     gene            485..1951
                     /locus_tag="VFN42_06025"
     CDS             485..1951
                     /locus_tag="VFN42_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HET8996207.1"
                     /translation="MPRAARALMVQGTGSDVGKSLLVAGLARAMSNRGLRVRPFKPQN
                     MSNNAAVTADGGEIGRAQALQARAARAAASVHMNPVLLKPQGQAGAQIVVQGRVTGQA
                     TAAAYQQLKPRLMVPVLDSYARLQAEADVILVEGAGSASEVNLRANDIANMGFARAVG
                     VPVVLVGDIDRGGVIAGLVGTAAVLDPEDAAMIVGFVVNKFRGDPSLFADGMTLIAAR
                     TGWAPLGLVPFFPEAARLPAEDSLALSQHLPRRPGAKLRVAVPLLPHIANFDDLDPLA
                     AEPAVDLVRVSYGEALPGDAQLVILPGSKATIADLAALRQAGLDIDIAAHLRRGGILL
                     GMCGGYQMLGTRIADPQGIEGPAGTAPGLGLLHVETVLSGDKRLLPARGRTADGAPLT
                     GYEMHLGITEGPDRSRPFARLEDGSPEGAISADGRVLGTYVHGLFAHDRQRGAWLARF
                     AAGAPALDYEASIDRTLNALADHLARHVDLDQLLRLMR"
BASE COUNT          336 a          724 c          740 g          325 t
ORIGIN      
        1 gcaaggggaa gtcgaccgcc gcctacggtc ttgtgctgcg catgctgggt catggcgggc
       61 gggtcggcgt cgtgcagttc atcaagggca cctggcagac cggcgagcgg accgctttcg
      121 ctgccttcgg ggaccgtgtc cgctggcaca gcatgggcga gggcttcacc tgggaaaccc
      181 aggaccgcgc ccgcgacatc gccgcggcgg agcgcgcctg gtctgtcgcg gccggcatgc
      241 tgcgcgagcc tgcgctcgac ttggtcgtgc tggatgagct gaacatcgcc ctgcgctacg
      301 actatctgca actcgcgacc gtgctggctg acctcgcagc ccgcccgcca cgccagcacg
      361 tcgtcgtcac cggccgcaac gcgaagcccg agctgatcga ggccgccgac ctggtcaccg
      421 aaatgggcgc ggtgaagcac catttcgcag ccggcgtccg ggcgcagaag ggaatcgaat
      481 actgatgccg cgggcggcgc gggccctgat ggtgcagggc accggttccg atgtcggcaa
      541 atcgctgctg gtggccggac tggcccgcgc catgtccaac cgggggttac gcgtgcggcc
      601 gttcaagccg cagaatatgt cgaacaatgc cgccgtcacg gcggatggcg gcgaaatcgg
      661 ccgagctcag gcgctacagg cccgtgccgc gcgtgcggcc gcctcggtgc acatgaaccc
      721 cgtgctactg aagccgcagg gccaggcagg cgcgcagatc gtggtgcagg gccgtgtcac
      781 cggccaggcc accgctgccg cataccagca gctgaagccc cgcctgatgg taccggtact
      841 ggacagctac gcgcggctgc aggccgaggc cgacgtgatc ctggtggaag gggcgggcag
      901 cgcgtccgaa gtgaatctgc gcgccaatga catcgccaac atggggtttg cccgcgccgt
      961 cggcgtgccg gtggttctgg tcggcgacat cgaccggggc ggcgtcattg ccggactggt
     1021 cggcaccgcg gccgtgctcg acccggagga cgccgccatg atcgtcggct ttgtcgtcaa
     1081 taaattccgc ggcgacccat cactgtttgc agacggcatg accctgatcg cggcccgcac
     1141 gggctgggcg ccgcttgggc tggtgccgtt cttccccgaa gcggcccgcc tgccggctga
     1201 ggattcgctt gcgctgagcc agcatctgcc acgccggccc ggagcgaagc tgcgggtggc
     1261 tgttcccctg ctgccgcaca tcgccaattt cgacgatctc gacccgctgg cggctgaacc
     1321 ggcggtggat ctggtgcgcg tgtcatatgg tgaagccctg cccggcgatg cacagctggt
     1381 gatccttcct ggctccaagg caacaatcgc ggacctggcg gcgctgcgcc aggccggact
     1441 cgacatcgac atcgccgcgc atctgcggcg cggcggcatt ctgctgggca tgtgcggcgg
     1501 ctaccagatg ctcggcacgc ggatcgccga cccgcagggc atagaaggcc cggccggcac
     1561 ggcccccgga ctgggcctgc tgcatgtcga gacggttcta tcaggcgaca agcgattgct
     1621 gccggcccgc ggccgcacag cagacggggc tccgctcacc gggtatgaaa tgcatctggg
     1681 aatcaccgaa ggcccggacc ggagccgccc tttcgcgcgg ctggaggatg gcagtccgga
     1741 aggcgccata tctgccgatg gccgcgtgct tggcacctat gtgcacggcc tgttcgccca
     1801 tgacaggcag cgtggcgcat ggctggcccg cttcgccgcc ggcgcaccgg cgctggacta
     1861 cgaagcatcg atcgaccgca cccttaatgc cctggccgat cacctggccc gccacgtcga
     1921 cctggatcag ctgcttcgcc tgatgcgctg agcaaccggg cgggaccgcc gcaagggagg
     1981 tcgcccaagc acgaatcccg ggacagccag ggggttcaaa agcttggtgc gcctgtgcgg
     2041 ctcggaccta ccataatccg gcagccagaa tggcgcccac cagcatggca tcggccagca
     2101 ggaacaggcg cagggcccgg ccgat
//
LOCUS       DASSZH010000280         2374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1404069, whole genome shotgun sequence.
ACCESSION   DASSZH010000280 DASSZH010000000
VERSION     DASSZH010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404069"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(614..>2374)
                     /locus_tag="VFN42_06030"
     CDS             complement(614..>2374)
                     /locus_tag="VFN42_06030"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HET8996208.1"
                     /translation="GAYKRIDGVASGALDPRHPANRGIALLDKAASNEHGLVAYRTGF
                     TVLTPADPAKGNHRLLYEVNNLGRIMLFANLCAGTMGNQPQTTAELGNALPLRLGFTL
                     VWSGWDPAAPASTGLSLQAPVATERDKPIVQRIREEFIAGTRRGDMATFPLSYEAADQ
                     DAQLTVRRTQTGERRPAAFEFVDARTLRLRDGARPEPGAIYELHYNATKPAVLGIGFA
                     ATRDLISHMHSRGADLLDFAPTHALAFGISQAGRYLRDHIAQGFNADEDGARVFDGVF
                     THVAGIGCVFHNTPFGQPFRTRTWHEDHDFPEVAFPFSAAFCEDPITGASGSLLRGDA
                     SDPKLIETNTSTEYWQKGASLLHSDPLGTQDLALPENVRGYLLAGTQHGGKAGMPRDN
                     GPCSNPRNWHDPMPAVRALLVALDAWVAEDKPPPDSILPRIADGTLVPAEQVAFPRIP
                     GLVPPTAANDAAPLGDWIDPQPPARAWRTLVPQMDADGNERAGIRLPDIAVPHGTFTG
                     WNLYREPYPAGELADRDGTFLAFAETRAQREQSGDPRASLEERYPTPAEHARRVRDCV
                     DALCRERLLLAEDAEVYRAG"
BASE COUNT          377 a          784 c          830 g          383 t
ORIGIN      
        1 caagccaata tcgatgcgct cagcaagatt ctcgccgaga aagcaggcaa ggttgcgaag
       61 gacaaggcca agtaccagag ctgcttcgat atggtcgatg ccggggagcg cgtgcgggcg
      121 cgctgtgtgg aactcgggcg ctaggctccc ggtcccgggt ccgcttccca gtgggcacaa
      181 cagggcaacg cgcaagaatg attgctgcag aactatcggc gtatgccacc ccgtcatggc
      241 cggcggaggc cggccatcca cgacttcttc aatgtggccg cggcaaagtc gtggatggcg
      301 ggcctgcgcc cgccacgacg gaacggagca gcgtgccacc agccgattcg ccagtttctg
      361 cgcctcgcct aactcatacc caccggcgga aaggattacc tatcggcggt ttcggcatcg
      421 tcatgccggc aaaggccggc atccacgact tctcgtgtct ccaacagcaa gccgcggatg
      481 gcaggccttc gtccattggc gccaacgtaa ggcgacactc actatctcaa ccggcacctg
      541 gcatcccgag tggaacgaca ccatcagccg cagacccaga ccctgaacgt agcggttatt
      601 cctgcgtgat gccttatcct gcgcgataca cctcggcatc ctccgccagc agcagccgct
      661 cgcggcacag cgcgtcaacg caatcccgca cgcggcgggc atgctctgcc ggcgtcggat
      721 agcgctcctc cagcgaagcg cgcggatcgc cgctctgttc ccgctgcgcg cgggtttcgg
      781 caaacgccag gaacgtgccg tcgcggtccg ccagttcgcc ggccggatag ggttcgcgat
      841 acaggttcca gccggtgaag gtgccgtgcg gcacggcaat gtcgggcagg cggatgccgg
      901 cgcgctcgtt gccgtcggca tccatctgcg gcaccagcgt gcgccaggcg cgcgccggcg
      961 gttgcgggtc gatccagtcg cccagcggcg cggcgtcatt tgcggcggtt ggggggacca
     1021 ggccgggaat gcgcgggaac gcgacctgtt cggccggcac cagcgtgccg tcggcgatgc
     1081 gcggcagaat actgtccggc gggggtttgt cctctgccac ccaggcgtcg agcgccacca
     1141 gcagcgctcg caccgccggc atcgggtcgt gccagttgcg cgggttgctg cacgggccat
     1201 tgtcgcgcgg catgccggcc ttgccgccgt gctgtgtgcc ggccagcagg tagccgcgca
     1261 cattctctgg cagcgccagg tcctgcgttc cgagcggatc ggagtgcagc agggaggcgc
     1321 ccttctgcca gtactcggtg gaggtgttgg tctcgatcag tttcggatcg ctggcatcgc
     1381 cgcgcagcag cgagccggac gcgccggtga tcgggtcctc gcagaacgcg gcggagaagg
     1441 ggaacgcgac ttccgggaag tcgtgatcct cgtgccaggt gcgggtgcgg aacggctggc
     1501 cgaagggcgt gttgtggaac acgcagccga tgccggccac gtgggtgaac acgccgtcga
     1561 acacgcgcgc accgtcctcg tccgcattga acccctgcgc gatgtggtcg cgcagatagc
     1621 gtcccgcctg ggaaatgccg aaagccagcg catgcgtcgg cgcgaaatcg agcaggtcgg
     1681 cgccgcggga atgcatgtgg ctgatcaggt cgcgcgtggc ggcgaagccg atgccgagca
     1741 cggcgggttt cgtggcgttg tagtggagtt cgtagatcgc gccgggctcc gggcgcgcgc
     1801 cgtcgcgcag ccgcagcgtg cgcgcatcga caaattcgaa cgcggccggc cggcgctcgc
     1861 cggtctgcgt gcggcgcacg gtcagttgcg cgtcctggtc cgcggcctca tagctcaacg
     1921 ggaaagtcgc catgtcgccg cgccgcgtgc cggcgatgaa ttcctcgcgg atgcgctgca
     1981 caatcggctt gtcgcgctcg gtggcgacgg gtgcctgcag cgacaggcca gttgacgcgg
     2041 gggccgccgg atcccagccg gaccacacca gcgtgaagcc gagccgcagc ggcagcgcat
     2101 tgcccaattc cgccgtggtc tgcggttggt tgcccatggt gccggcgcac agattggcaa
     2161 acagcatgat gcggccgagg ttgttcacct catacagcag gcggtgattg cccttggccg
     2221 gatcggccgg agtcagcacc gtgaacccgg tccgatacgc caccagcccg tgttcgttgc
     2281 tcgccgcctt gtccagcagg gcaatgccgc ggttcgcggg atgccgcgga tcgagcgcgc
     2341 cgctggcgac gccgtcgatg cgcttgtagg cgcc
//
LOCUS       DASSZH010000281         2408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1353018, whole genome shotgun sequence.
ACCESSION   DASSZH010000281 DASSZH010000000
VERSION     DASSZH010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353018"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..182)
                     /gene="rpmG"
                     /locus_tag="VFN42_06035"
     CDS             complement(15..182)
                     /gene="rpmG"
                     /locus_tag="VFN42_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET8996209.1"
                     /translation="MAKTNTVQIKLISSADTGYFYVTKKNARTQTGKLELKKYDPVAR
                     KHVLFKEAKIK"
     gene            complement(251..1258)
                     /locus_tag="VFN42_06040"
     CDS             complement(251..1258)
                     /locus_tag="VFN42_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996210.1"
                     /translation="MRRRQFGLGLAALAASARQARGTDDFLVRIARRNAFSELPLMVM
                     QANRLIESHAAKLGLLSLEVEWRISRGSEATIDDLFANRIDFGVLDAPELVDLWDRTM
                     TSTGVMRALSCVALQPFLLLTRNPAVQSLADFTPKDSIAVSRVRLSTPAVCLEMAAAR
                     QWGPGLYSRLDPLTFSLPDRQAEESLLAARPPVNTHYAPAPYYYDELATRGIHLVVKS
                     NDTLGGPHANGLLVAAPYFDPGTTAVAQAVLAAQEEANLLIRQQPVAAARTYLLLTGD
                     NRRPDDLVGMIGDPDIAWTTVPQRLMEFASFMHKVGRLEHMPASWKDLMLPEGRSGNG
                     S"
     gene            complement(1288..>2408)
                     /locus_tag="VFN42_06045"
     CDS             complement(1288..>2408)
                     /locus_tag="VFN42_06045"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET8996211.1"
                     /translation="GARFRFFHLGTWPGGKSGALNFALRETAPDAAVIAALDCGTVVQ
                     PDWLRRMLGYFADPAIGFAWSPQADPGASPWIAATAARNNPEVVPVQAMTLVRRTALE
                     SAGGWAEGTLAGNTELGLRLLQRGWHGAHSPEGFGRAVGAQGFAAASRRRFRQAYGAM
                     QICRIHAHALLSPFDPALTLHQRETLVAAWLPELADALCLLLLLAALAWSAGLILNPR
                     MVSFPTLPLTLAPILLCALTVVRAVHQCPGPAGWHACTRAALAGLALSHASGGGVWQA
                     VLGGEPDAEERQPAYAGMAQRLAPVRSELVLLVLGWSAAAGIAVARDMAQPQAVAWCV
                     MLLVQSLPCLAAVVAALRAGPVQDANRISRVPHGAVPL"
BASE COUNT          398 a          841 c          827 g          342 t
ORIGIN      
        1 gccggcgccg ggcgctactt gatcttggct tccttgaaca ggacgtgctt gcgcgcgacc
       61 gggtcgtatt tcttcagctc cagcttgccg gtctgggtgc gggcgttctt cttcgtcacg
      121 tagaagtacc cggtatccgc gctggagatg agcttgatct gaacggtgtt ggtcttggcc
      181 atggcatgat cctcggaagg ggcggaacat gctcatccaa ggggtagagg tcaagccggg
      241 tttccccggg tcagctaccg tttccggagc ggccctccgg cagcatcagg tccttccagg
      301 aggccggcat atgctccagc cggcccacct tgtgcataaa gctggcaaat tccatcagcc
      361 gctgcggcac cgtggtccag gcgatgtccg gatcgccgat catccccacc agatcatccg
      421 gccggcggtt gtcgccggtc agcagcaggt aggttcgtgc cgcagccacc ggctgctgcc
      481 tgatgaggag gttcgcctcc tcctgggccg ccagcacggc ctgcgcgacg gccgtggtgc
      541 ccggatcgaa atagggggcc gccaccagca ggccattggc gtgtgggccg cccagcgtgt
      601 cattcgattt cacaaccaga tggatgccgc gcgtcgccag ctcgtcataa tagtatgggg
      661 ccggagcgta atgcgtgttc accggcggcc gggcggcgag caggctttcc tccgcctgcc
      721 ggtcgggcag gctgaacgtc agcggatcca gccgggaata gagccccggg ccccactgtc
      781 tcgcggccgc catctccagg cagacggcgg gtgtggacag cctcacccgg gaaaccgcga
      841 tgctgtcctt gggcgtgaaa tccgccaggc tctgcacggc cggattgcgg gtgagcagca
      901 ggaagggttg cagcgcgacg caactcaggg cgcgcatcac cccggttgag gtcatcgtcc
      961 gatcccacaa atccaccagt tccggtgcgt ccagcacgcc gaaatcaatg cggttggcaa
     1021 acaggtcgtc gatggtcgcc tcggacccgc gtgagatccg ccactccacc tccagggaca
     1081 gcaagcccag cttcgccgcg tggctctcga tcagccggtt ggcctgcatc accatcagcg
     1141 gcagttccga gaacgcattg cgccgagcaa tgcgcaccag gaaatcgtcg gtgcctcgcg
     1201 cctggcgcgc cgaggccgcc agggcagcca gccccaggcc gaactggcgt cgccgcatgg
     1261 aacctccccc gccaatgcga gggatttcta tagcgggacg gcaccgtggg gaacgcggga
     1321 aatccggttt gcgtcctgca ccgggcccgc ccgcagcgcc gccaccaccg ccgccaggca
     1381 aggcagcgac tggaccagca gcatgacgca ccaggcgacg gcctgcggct gcgccatgtc
     1441 gcgggccacg gcgatgccgg cggccgcgct ccagcccagg accagcagca ccagttcgga
     1501 gcgcaccggg gccaggcgct gcgccatgcc ggcgtaagcc ggctgccgct cctccgcatc
     1561 cggctccccg cccagcaccg cctgccagac accgccgccg ctggcgtggc tcaaggccag
     1621 cccggccagt gccgctctcg tgcaggcgtg ccaaccagcc ggaccggggc actggtgaac
     1681 cgcccgtacg acggtcagcg cgcacagcag gatcggggcc agcgtgaggg gcagcgtggg
     1741 gaagctgacc atgcgcgggt tgaggatcag cccggccgac cacgccagcg ccgccagcag
     1801 caacagcagg cagagcgcat cggccagctc gggcagccac gcggcaacga gggtctcgcg
     1861 ctggtgcagg gtcagcgcag ggtcgaaggg cgacagcaag gcgtgtgcgt ggatgcggca
     1921 gatctgcatg gcgccatagg cctggcggaa gcgccggcgg gatgccgcgg cgaagccctg
     1981 cgcaccaact gcacgcccga aaccttccgg cgaatgcgcc ccgtgccagc cgcgttgcag
     2041 caggcggagc cccagctcgg tattcccggc cagcgtgcct tccgcccagc cgccggcgga
     2101 ctcgagggcg gtgcgccgga ccagcgtcat ggcctggacg gggacgacct cggggttgtt
     2161 ccgtgccgct gttgccgcaa tccagggcga agcgccggga tccgcctgcg gcgaccaggc
     2221 gaaaccgatc gccgggtcgg caaaataccc cagcatgcgg cgcagccaat ccggctgcac
     2281 caccgtgccg cagtccagcg cggcgatcac cgcggcgtcc ggcgcggtct cgcgcagcgc
     2341 gaagttcagc gcgccggact tgccgccggg ccaggtgccc agatggaaga agcggaaccg
     2401 ggcgccga
//
LOCUS       DASSZH010000282         2573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_160122, whole genome shotgun sequence.
ACCESSION   DASSZH010000282 DASSZH010000000
VERSION     DASSZH010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160122"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VFN42_06050"
     CDS             complement(<1..899)
                     /locus_tag="VFN42_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET8996212.1"
                     /translation="MTQTLQTNVAIVGAGPVGLFAVFELGMLQLSSVLIDALSEVGGQ
                     CAALYPEKPIYDIPAHPAIAAGDLITALEQQIAPFHAPRLLGRRVQGLAGGPGNFTLT
                     TDQGDIVQAKAVLIAAGAGAFGPNRPPLEGLPAFEATGAVQYYVRRREDLRGKRVVIA
                     GGGDSAVDWALALKDIAKVAVVHRRPKFRAAPETSAQLEAAAAGGELEMVIPYQLHAL
                     HGDAGHLESVEVATLRGETRRLPADVLLPFFGLSMDLGPIAAWGLDSELHHVKVDPTT
                     CRTSLPGVFAIGDVARYPGKLKL"
     gene            complement(947..2017)
                     /locus_tag="VFN42_06055"
     CDS             complement(947..2017)
                     /locus_tag="VFN42_06055"
                     /inference="COORDINATES: protein motif:HMM:NF038122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NF038122 family metalloprotease"
                     /protein_id="HET8996213.1"
                     /translation="MRRLRRHRLRHAVSAGSVALLCAPLLLGLTAQPAAALSIIPNFD
                     STITSDPNAAAIEAAITLALSPYAMFSDPVTVNILYQASHAGTSSYLGESGSAFYQNS
                     YAAYTGALATDAASYGNSVEQSGVTHLSSGNVAQSVLATAADLRALGCGGCAGTISSI
                     NLSNNTLVTGGSYDGAIAINLSQPLQFSRNANGTINGGFYDAISVIQHETDEVLGIGG
                     SGSILNDVGSGSTPPSIGGQTTIGALDLFRYSAAGTPSLTTSGSASSYFSLDGGTTHI
                     ASFNQSSNGDFGDWLVGTGHVQDAFTPSGQVANVSATSPEGLALQAIGYDVPEPASLA
                     LLAVGAAFVTGLRRRPRRRIAA"
     gene            2270..>2573
                     /locus_tag="VFN42_06060"
     CDS             2270..>2573
                     /locus_tag="VFN42_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996214.1"
                     /translation="MLARPAERAGYTDPEPPMYMNAFAETVQPLDMTRTDEFRLLLAS
                     AADLGIPHAPDVPYRSRNIVLRQLRFHFLEWGDPGAPPLVLLHGGHQSAHSWDLVRL"
BASE COUNT          402 a          833 c          897 g          441 t
ORIGIN      
        1 atcagcttca gcttgcccgg gtagcgcgcc acgtcgccga tcgcgaacac gcccggcagg
       61 ctggttcggc aggtggtggg atcgaccttc acatggtgca gctcgctgtc cagcccccag
      121 gcggcgatcg ggcccaggtc catcgacagg ccgaagaacg gcagcagcac gtcggcgggc
      181 aggcggcggg tctcgccgcg gagggtggcg acctccaccg attccaggtg gccggcatcg
      241 ccgtgcagcg cgtgcagctg gtagggaatc accatttcca gctcgccacc cgcggcggcg
      301 gcttccagtt gcgcgctggt ctccggcgcg gcgcggaatt tcgggcggcg atgcaccacg
      361 gcgaccttcg ctatgtcctt cagtgccagc gcccagtcca ctgcggaatc cccgccgccg
      421 gcgatcacca cgcgcttgcc gcgcagatcc tcccggcggc gcacgtagta ctgcaccgcg
      481 ccggtggctt cgaacgccgg caatccctcc agcggcggcc ggttcggtcc gaaggcgccg
      541 gccccggcgg cgatcagcac cgctttcgcc tgcacgatgt cgccctggtc ggtggtcagg
      601 gtgaaattcc ctggtccgcc agccagcccc tgcacccggc ggccgagcag ccgcggtgcg
      661 tggaatgggg cgatctgctg ctccagcgcg gtgatcaggt cgccggcggc gatcgccgga
      721 tgggccggaa tgtcatagat cggcttttcc gggtacagcg cggcgcactg cccgccaact
      781 tcggacagcg cgtcgatcag cacgctggac agctgcagca tgccgagttc gaacaccgca
      841 aacaggccga cggggccggc cccgacgatc gccacgttgg tctggagtgt ctgtgtcatg
      901 ccgagcgggt acgccgcttg gggcaggcga tcaagcgggc aggcggtcag gcggcaatcc
      961 gccgccgcgg ccggcgccgc agtcccgtga caaacgcggc gccgacagcc agcagcgcca
     1021 ggctggccgg ctccgggacg tcatagccga tcgcctgcaa ggccagccct tccggcgatg
     1081 tcgcggagac attggcgacc tggccggagg gggtgaaggc gtcctgcacg tgcccggtgc
     1141 ccaccagcca gtcgccgaaa tcgccgttgc tgctctgatt gaagctggcg atgtgcgtcg
     1201 tgccgccatc cagcgagaaa tacgagctgg cagagccgct ggtggtcagg ctgggtgtgc
     1261 cggcggcgga atagcggaac aggtcgagcg ccccgatcgt ggtttgacca ccgatgctgg
     1321 gcggcgtgct gccggagccg acatcgttga ggatggagcc ggaaccgccg atccccagca
     1381 cctcgtcggt ttcgtgctgg atgacgctga tcgcgtcgta gaagccgccg ttgatggtgc
     1441 cgttggcatt gcggctgaac tgcagcggtt ggctgaggtt gatggcgatg gcgccgtcat
     1501 agctgccgcc agtgaccagc gtgttgttgc tcaggttgat gctgctgatg gtgccggcac
     1561 atcctccgca gccgagggcg cgcaggtcgg cggcggtggc aagcacgctt tgcgccacgt
     1621 tgccgctgga caggtgggtg actcccgatt gctcgacgct gttgccgtag ctggcggcat
     1681 ccgtcgcgag cgctcccgta taggccgcgt agctattctg atagaatgcg ctgccgctct
     1741 cgcccaggta gctgctggta ccggcatggg acgcctggta caggatattc accgtgaccg
     1801 gatcgctgaa catcgcgtag ggcgacaagg ccagcgtgat cgcggcttcg atcgcggcgg
     1861 cattcgggtc gctggtgatc gtgctgtcga agttgggaat gatgctcaga gccgcagccg
     1921 gttgtgccgt caggcccagc agcaaggggg cgcagagcag ggcgacggaa ccggcagaga
     1981 cggcatggcg aagccggtgc cgacggagac gcctcatcag tgtacctcag tttcgagaat
     2041 cgatcgggtt gtaatcagat tggatagcgc cgtgcaacaa cgtttcggct cacgcgaatc
     2101 cttcacaaat gataccatac aggcagaaca gatgaatttt tgagtgcgat gcgggccggt
     2161 tcgccccggt ttgatttcgc tattgcaatt ccggctttgt tcgctgccgc gcagcgccgg
     2221 tttgacgcgc cggcagagcc gggcgcttgc gcgccgggcg ggaagcttca tgctggcccg
     2281 cccggcggaa cgtgccgggt acaccgatcc ggaaccgccc atgtatatga acgcgttcgc
     2341 cgaaaccgtg cagccgctgg acatgacgcg gacggacgag tttcgactgt tgctcgccag
     2401 cgccgccgac cttggcatcc cccacgcgcc cgacgtgccg taccgctctc gcaacatcgt
     2461 gctgcggcag ctacggtttc atttcctgga atggggcgat cccggcgcgc cgccgctggt
     2521 gctgctgcat ggcgggcacc agtcggcgca ttcctgggac ctggtccgcc tgc
//
LOCUS       DASSZH010000283         3232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_978212, whole genome shotgun sequence.
ACCESSION   DASSZH010000283 DASSZH010000000
VERSION     DASSZH010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978212"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VFN42_06065"
     CDS             complement(<1..702)
                     /locus_tag="VFN42_06065"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HET8996215.1"
                     /translation="MSDQALSRDIAAPPIATHRRHLTRRHLGISALAAAVLLAGGAYG
                     YRWWTVGRFVETTDDAYVGTNVTPIAPHIAGFVQQILVRDNQHVHAGQTLIRLDPQDF
                     QAALQHAEAVLQAREAALANLVAKRVLQHSVIAGADADLAAKQAQAAFTAEDAARYHT
                     LALTAAGSRQSAEKSLAADRAAHAAVRAAQAAQQAAVQQVAVLDTEIAAAKADVAQAQ
                     ADVHTARLNLGYTAIT"
     gene            complement(699..1208)
                     /locus_tag="VFN42_06070"
     CDS             complement(699..1208)
                     /locus_tag="VFN42_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET8996216.1"
                     /translation="MQRKSFGNMSCPIARSLERVGEWWSILILRDAFHGLTQFDQFQK
                     SLGIAPNMLTRRLNALVESGLLERRRYSERPPRDEYVLTERGRDFRPVLWALLAWGNK
                     HFAPEGASVLVVDAETGKPADPVLVDRNTGKVMSPEAFHSAPGPAADDHTRGRLHRGA
                     AARTAENAS"
     gene            1357..1683
                     /locus_tag="VFN42_06075"
     CDS             1357..1683
                     /locus_tag="VFN42_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996217.1"
                     /translation="MRIATEALVDNAVERSGDGPYNSGLEARVSRLETDMSEVKAVLV
                     RLESAVTDLRVIVARIDAQINAQLPYLATKADLAEKPSKTYMWGILGALLTAYACGLA
                     ALAILK"
     gene            complement(1703..2425)
                     /locus_tag="VFN42_06080"
     CDS             complement(1703..2425)
                     /locus_tag="VFN42_06080"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET8996218.1"
                     /translation="MQARDIMTPDVICVAPDTTLAEVVRTMLEHRISAVPVLEDGTLV
                     GIISEGDLLRRVETHTDAPPSRWLELLHSGDMRAADYVKTHGRRAAEVMTRMVITVGE
                     DASVSQIARILASHRFRRVPVMRGERLVGIVSRANLLQALASRIAAPAEPATMDDRCI
                     RDTLLTEIARQSWAAPGDGSVTVSDGVVHLWGTVESEAKRQALVVTAENIPGVRAVED
                     HMQAPAIVDPLDRPNWPQPATP"
     gene            complement(2509..>3232)
                     /locus_tag="VFN42_06085"
     CDS             complement(2509..>3232)
                     /locus_tag="VFN42_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007425080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HET8996219.1"
                     /translation="PKAEAMCGRYAVFLPPDAIAALFRAGSPVPNWAPTWNMAPTRDA
                     PVVRLHPTARERRIDLLRWGLLPHWAKVAKFGRQPVNARAESLATGQMFRDAYAHRRC
                     LIPADVFYEWQVADGGKRPWAIARADGQTMAFAGVWEGWRGPDGLVIRTFAIITTEAN
                     DIMRPLHARMPLILEAPDWPLWLGETEGDAAGLLRPSKTELRCWRISSSVNHVRNDGP
                     ALLDPLLPEPAPALFPADSERG"
BASE COUNT          487 a         1057 c         1138 g          550 t
ORIGIN      
        1 ggtgatcgcg gtgtagccca ggttgagccg cgcggtatgc acatcggcct gggcctgcgc
       61 gacgtcggcc ttggccgcgg cgatttccgt atccaggacc gccacctgct gtaccgcggc
      121 ctgttgcgcc gcctgcgcgg cgcgcaccgc cgcgtgcgcc gcgcggtcgg cggccaggga
      181 tttctcggcg ctctgacggg agccggccgc cgtcagtgcc agcgtgtgat agcgtgcggc
      241 gtcttccgcg gtgaaggccg cttgcgcctg cttggcagcg agatcggcgt cggcgccggc
      301 gatcaccgaa tgctgcagca cgcgtttggc cacaagattt gccagcgcgg cctcgcgggc
      361 ctgcagcacc gcttctgcat gctgcagcgc ggcctggaag tcctgtggat caaggcggat
      421 caaggtctgg ccggcgtgta cgtgctggtt gtcgcgtacc aggatctgct gcacgaaacc
      481 ggcgatgtgc ggcgcgattg gcgtgacgtt ggtccccaca taggcatcgt cggtcgtctc
      541 cacgaagcgg ccgactgtcc accagcgata gccataagca ccgccggcca gcagcacggc
      601 ggcggccagc gcggatatcc cgagatgccg gcgtgtgaga tggcggcggt gagtcgcgat
      661 gggcggcgcc gcgatgtcgc gagagagggc ctgatcgctc atgatgcatt ctccgcagtg
      721 cgggctgcgg cgccgcgatg gaggcggccg cgtgtatggt catcggcagc cgggccgggg
      781 gcgctgtgga aggcttccgg cgacatcact ttcccggtgt tgcggtccac cagaaccgga
      841 tcggcgggct ttccggtttc ggcgtccacc accaggacgc tcgcgccctc cggcgcgaaa
      901 tgcttgttgc cccaggcgag cagagcccag agcaccgggc ggaagtcgcg cccccgttcg
      961 gtcagcacgt actcgtcgcg tggcgggcgt tcgctgtagc ggcggcgttc cagcaggcca
     1021 ctctccacca gggcgttgag gcggcgggtc agcatattgg gggcgatccc caggctcttc
     1081 tggaactgat cgaactgggt caggccgtgg aaggcgtccc gcaggatcag gatgctccac
     1141 cattcaccga cccgctccag gctgcgggcg atggggcagg acatgttgcc gaagctcttg
     1201 cgctgcatga tgggcctcgc agtcggttgc gtgatgcaag tcactatggg gtggtgactt
     1261 tcattatgca aggcaaaaat gggcgcccct ccggcggtcg gagatgccgg tggacgatcg
     1321 cttgagcttt gattaaccgg ccgcaggcat gctgccgtgc gcatcgccac ggaggctctg
     1381 gtggacaacg cggttgaacg gtccggggac ggcccctaca actctggcct ggaagcccgg
     1441 gtatcccggc tggagaccga tatgagcgag gtcaaagccg tattggtacg gctggaatcc
     1501 gccgtcaccg acctgcgggt catcgtggcg cgaattgatg cgcagattaa cgcgcagctc
     1561 ccgtaccttg cgaccaaggc agacctcgcc gagaagccga gcaaaaccta tatgtggggc
     1621 atactcggcg cgctgttgac cgcctacgcc tgtgggcttg ctgcgctggc gatcctgaaa
     1681 tagcgcgccg tcggcggcga cctcagggtg tcgcgggttg cggccagttg ggccggtcga
     1741 gcgggtccac tatcgcgggg gcctgcatat ggtcctccac ggcgcgtact cccgggatgt
     1801 tctccgccgt tacgaccagc gcctggcgct ttgcttccga ttccaccgta ccccatagat
     1861 gcacgacacc gtcgctgacc gtcacgctgc catcgcccgg cgccgcccag ctttgccgtg
     1921 cgatctcggt cagcagcgtg tcgcggatgc agcggtcgtc catcgtcgct ggttcggccg
     1981 gggccgcaat ccggctcgcc agggcctgca gcagattggc ccggctgacg atgcccacca
     2041 gtcgctcgcc gcgcatcacc ggcacgcggc ggaaccggtg cgaggcgagg atgcgggcga
     2101 tctgcgagac cgaggcgtcc tcaccgacgg tgatcaccat gcgggtcatg acctcggccg
     2161 cgcgtcgtcc atgcgtcttc acgtaatcgg cggcacgcat gtcgccggaa tgcagcaatt
     2221 ccagccagcg cgagggcggt gcgtcggtgt gggtttccac cctgcgcagc aggtcgccct
     2281 cactgatgat gccgaccagc gtgccatcct ccagcaccgg gaccgcgctg atgcgatgct
     2341 ccagcatggt gcgcaccacc tccgccagcg tggtgtccgg tgccacgcag atcacgtccg
     2401 gcgtcatgat atcgcgggct tgcatgccgc atctccctga caggcaaccc tggctactgc
     2461 ggatccggca cggccgcgtt gatgcaggtc aagggcagtc ggcgcggatc aaccgcgttc
     2521 ggagtcggcc ggaaacagtg ccggcgcggg ttctggcagc agcggatcga gcagtgccgg
     2581 gccgtcattg cgcacgtggt tgacgctgga actgatgcgc cagcaccgca attccgtctt
     2641 gctggggcgc agcagtccgg cggcatcgcc ctcggtctcg ccaagccaga gcggccagtc
     2701 gggtgcctcc aggatcagcg gcatgcgggc gtgcagcggg cgcatgatgt catttgcttc
     2761 ggtggtgatg atggcgaagg tgcggatcac caggccgtcg gggccgcgcc agccctccca
     2821 tacgccggcg aacgccatcg tctgcccgtc ggcgcgggcg atcgcccagg ggcgcttgcc
     2881 accgtccgcc acctgccatt cgtagaacac gtcggccgga atcaggcagc ggcgatgcgc
     2941 ataggcgtcg cgaaacatct ggccggtggc cagggattcc gcgcgggcgt ttaccggctg
     3001 gcggccgaat ttcgccacct tggcccaatg cggcagcagt ccccagcgca gcaggtcgat
     3061 ccggcgttcc cgcgcggtag gatgcagccg aacgaccggc gcatcccggg tgggcgccat
     3121 gttccaggtc ggcgcccagt tcggtacggg tgacccggcg cggaacaggg cggcgatggc
     3181 atcgggcggg agaaagacgg cgtagcggcc gcacatcgct tcggctttcg gc
//
LOCUS       DASSZH010000284         3579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_381849, whole genome shotgun sequence.
ACCESSION   DASSZH010000284 DASSZH010000000
VERSION     DASSZH010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381849"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VFN42_06090"
     CDS             <1..341
                     /locus_tag="VFN42_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HET8996220.1"
                     /translation="WPAPQAALARRDPADPRVAERFELFVCGIELANAFVELTDAAEQ
                     RARFQADRARRHALYGADWPLDEDFLAALSHGMPPSAGIALGFDRLAMLAAGADRIAQ
                     VLWLPPADLV"
     gene            390..746
                     /locus_tag="VFN42_06095"
     CDS             390..746
                     /locus_tag="VFN42_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996221.1"
                     /translation="MRRTLLLLLPVLLGGCANYLAQRQAFLSQFVGKPDTLLVQKLGV
                     PTRSFTTDGVQYLAYTESRVDIIPGTPGFGWGSPFWGWYGGGFPPQAVTRACETTFAV
                     TKGIVQSYTLRGNACG"
     gene            815..1483
                     /locus_tag="VFN42_06100"
     CDS             815..1483
                     /locus_tag="VFN42_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010507101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET8996222.1"
                     /translation="MRVLYHLPLSPYARKVRLALAEKRLPFELRTEKAWERRPEYLEL
                     NPAGMVPTLVEDSGLVIPDSSAICEYLEEAYPDTSLLGRTLAERVEVRRLVAWFDGKF
                     AAEVTSGLYGEKFMKRLTKRGNPDPGAIRTGYVALRYHMEYLGWLAETRKWLAGAELS
                     LADFAAAAHLSALDFIGDVDWSLSPPAKDWYARIKSRPSFRGLLADRVPGTTPPEHYA
                     DLDF"
     gene            1604..2014
                     /locus_tag="VFN42_06105"
     CDS             1604..2014
                     /locus_tag="VFN42_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996223.1"
                     /translation="MTLPFALPDWLPWWVPVLLMIPALLYALAFLFMPFSVIGVKGRL
                     DVLDARLDEIQNEIRHLALRMPEAAQQVDFEEVYAPEPRVEPSLRAAPASRPPIPPAL
                     HELEPEDQAPAADRPAPAPTARPLRREPRLDWPR"
     gene            2149..3297
                     /locus_tag="VFN42_06110"
     CDS             2149..3297
                     /locus_tag="VFN42_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HET8996224.1"
                     /translation="MQPEPFTLSVPDTALADLRQRLANTRFPDQAPDAPWAYGTDLDA
                     MRGLVAYWRDRFDWRAQEARLNDFPQFKVKLSGIDLHFLHVPGVGPAPTPLLLSHGWP
                     GSVFEFLELIPRLTNPARFGGDPADACTVVAPSLPGYGLSFAPGQKRFSLPEIGDCFA
                     ALMHDVLGYRRYVAQGGDWGSFVTSYLGAAHAERIIGIHLNMLPLRRDPAMLSNATPE
                     EQRYLAELAHWLKEETGYQWIQGTRPQTLAYGLTDSPAGLAAWILEKFRAWSDCGGEV
                     ESVFSRDALLANISFYWFTGAIGSSFWPYYARMHGPWPIPDGPITVPMGYCAFPHEIL
                     RPPRSMAERVFTDIRRWSVMPRGGHFAAMEQPEALAEELQAFVRRLRA"
BASE COUNT          519 a         1229 c         1209 g          622 t
ORIGIN      
        1 actggccggc accgcaagcc gcgctggcgc gccgcgatcc tgccgatccg cgcgtcgccg
       61 aacgcttcga gctgttcgtc tgcggcatcg aactggccaa tgcctttgtg gaactcaccg
      121 atgccgcgga gcagcgcgcc cggtttcagg cggatcgcgc ccgccgccac gcgctgtacg
      181 gcgcggactg gccgctggac gaggatttcc tggccgccct gtcgcatggc atgccgccct
      241 ccgccggcat cgccctcggc ttcgaccggc tggcgatgct ggccgccggt gccgaccgga
      301 tcgcccaggt gctctggctg ccgccggcag accttgttta atcgcgatgg cgttccgaaa
      361 ttccctccga ccaggccgga gacaaaccga tgcgccgaac cctgctgttg ctgttgcctg
      421 tgctgctcgg cggctgcgcg aactatctgg cgcagcggca ggcattcctg tcgcagttcg
      481 tcggcaagcc ggacactctg ctggtgcaga agctcggcgt gccgacccgc agcttcacga
      541 ccgatggcgt gcagtacctg gcctataccg agagccgtgt ggacatcatt ccgggcacgc
      601 ccggcttcgg ctggggctcg cctttctggg gttggtacgg cggcggcttc ccgccgcagg
      661 cggtcacgcg cgcgtgtgaa accaccttcg cggtcaccaa gggcatcgtg cagtcctata
      721 ccctgcgcgg caacgcctgc ggttagggcc cacggcatcg cgactcgtcc cggcgcttgg
      781 cgcggcggcg aactcggtgc cataagcagc cgcgatgcgc gtgctctatc acctgccttt
      841 gtccccctac gctcgcaagg tgcgtctcgc cctggccgag aagcggcttc ccttcgaatt
      901 gcgtacggaa aaggcgtggg aacgccgtcc ggaatatctg gagctgaacc cggccgggat
      961 ggttcccacc ctggtggagg acagcggcct ggtcatcccg gattccagcg cgatctgcga
     1021 atacctggag gaagcctatc ccgacaccag cctgctgggc cgcaccctgg cggagcgggt
     1081 ggaggtgcgc cggctggtcg cctggttcga cggcaagttc gccgcggaag tgaccagcgg
     1141 gctgtacggc gagaagttca tgaagcgcct gaccaagcgc ggcaatccgg acccgggcgc
     1201 gatacgcacc ggctatgtcg cgctgcgcta ccacatggaa tacctgggct ggctcgcgga
     1261 gacgcggaaa tggctggcgg gcgcggagct gtcgctggcg gatttcgccg cggccgcgca
     1321 tctctcggcg ctggatttca tcggcgacgt ggactggtcg ctgtcgcccc ccgccaagga
     1381 ttggtacgcc cgcatcaagt cgcgtcccag ctttcgcggc cttttggccg accgggtgcc
     1441 cggcaccacc ccgccggaac actacgccga cctggatttc tgagcggcgt ggcgggcccg
     1501 cgggcaggct ggcaggacgc ctgggccaca gctccgttca ctaacgctgg atcggcaaca
     1561 ggttcatgga tcgcatgatg cttgaggtgc taatttgccg cacatgaccc tgccgttcgc
     1621 cctgcccgac tggttgccct ggtgggtgcc cgtcctgctg atgatcccgg cgctgctgta
     1681 cgcgctggcc ttcctgttca tgccgttcag cgtgatcggg gtaaagggcc ggctggacgt
     1741 gctggatgcc cgactggacg aaatccagaa cgagatccgc catctggcgc tgcgcatgcc
     1801 ggaagcggcg cagcaggtgg atttcgagga ggtctacgcg ccggaaccgc gcgtcgagcc
     1861 gagcctgcgc gccgctccgg cgtcccgccc gccgattccg ccggcgctgc acgagctgga
     1921 gccggaagac caagctcccg ctgcggatcg ccccgcgccg gctccgaccg ctcggccgct
     1981 gcggcgggaa ccacggctgg actggccgcg ctagcaggcc ggcgcggcgg ccacggctct
     2041 cccggagcga gcgcacggcc gatcggaccc ctccacgccg tccgtgttca tccgggcgca
     2101 tccgtggttc catcctcctt cgcgtgcccc acgccgggag gaccgatcat gcagcccgag
     2161 ccgttcaccc tgtccgtgcc ggacaccgca ctggccgacc tgcggcagcg cctggccaac
     2221 acgcggtttc cggaccaggc gcctgatgcg ccctgggcct atggtaccga cctggatgcc
     2281 atgcgcggtc tggtcgccta ctggcgcgac cgcttcgatt ggcgcgcaca ggaagctcgg
     2341 ctgaacgact ttccgcagtt caaggtgaag ctgtcgggca tcgacctgca tttcctgcac
     2401 gtcccgggtg ttggcccggc gccgaccccg ctcctgctgt cgcatggctg gccgggctcg
     2461 gtgttcgagt tcctggagct gattccgcgg ctgacgaatc cggcgcgctt cggcggcgat
     2521 ccggcggacg cctgcaccgt ggtggcgcca tccttgcccg gctacgggct gtccttcgcg
     2581 ccggggcaga agcggttcag cctgccggag atcggcgact gcttcgccgc gctgatgcac
     2641 gacgtgctgg gctatcgccg ctacgtcgcg cagggcggtg attggggttc cttcgtcacc
     2701 tcctatctcg gtgcggcgca tgccgagcgg atcatcggca ttcacctcaa catgctgccg
     2761 ctgcggcgcg atccggccat gctcagcaac gccacgccgg aggagcagcg ctacctggcg
     2821 gagctggcgc actggctgaa ggaggagacc ggctatcaat ggatccaggg tacgcggccg
     2881 cagaccctgg cctatggcct gaccgattct ccggccgggc tggcggcctg gatcctggag
     2941 aagttccgcg cctggtcgga ttgcggcggc gaggtggaga gcgtgttcag ccgggacgcg
     3001 ctgctggcga atatcagctt ctactggttc accggcgcga tcggatcctc gttctggccg
     3061 tactatgccc gcatgcacgg accgtggccc attccggatg gtccgatcac cgtgcctatg
     3121 gggtattgcg cgttcccgca cgaaatcctg cgcccgccgc gctccatggc ggaacgggtc
     3181 tttacggata tccggcgttg gagcgtgatg ccgcgtggcg gccacttcgc cgccatggaa
     3241 cagccggagg cgttggcgga ggagttgcag gcattcgtgc ggcgactgcg cgcatagggg
     3301 ggacgaaatt cgttgctcag caagccgata tcgaacgccg gaccacccga gccgtacgag
     3361 agcagatccg cgctaccgcc cgaataggga tggtcccaat ccggttcgcc catcagcgcg
     3421 cgattcaccg cggtgagcat ctcgttacaa tagcccagca cgatctcggt ggccttgtgc
     3481 cacggccctc gggtgagatt gtccggatcg aaggcttcgc cccggggtgc cagaatggaa
     3541 taccgcgagg caatccagcc cgggcgccgc cgacccggc
//
LOCUS       DASSZH010000285         4538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_637553, whole genome shotgun sequence.
ACCESSION   DASSZH010000285 DASSZH010000000
VERSION     DASSZH010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637553"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VFN42_06115"
     CDS             complement(<1..469)
                     /locus_tag="VFN42_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET8996225.1"
                     /translation="MSAAAQVWGAPEGWDAFLLARRRAEHKGSVLHVARDDARMARVA
                     ESLAFVLPEAEILRFPAWDCLPYDRVSPNAALVAERLATLTRLLEKPDRPRIVLTTVN
                     ALVQRVPPRAAFAGASMELRVNGTVQPEKLAEFLESHGYGRAGTVMEPGEYAMR"
     gene            complement(466..735)
                     /locus_tag="VFN42_06120"
     CDS             complement(466..735)
                     /locus_tag="VFN42_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HET8996226.1"
                     /translation="MTDPDSAALETRRRRLLYRATHRGTFENDLMLGGFVRANLDGLA
                     PPELDALEELLELPDPDLADWLTGRRPMPADAPPMLLRIRDVLGR"
     gene            889..2121
                     /locus_tag="VFN42_06125"
     CDS             889..2121
                     /locus_tag="VFN42_06125"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8996227.1"
                     /translation="MDADPARIVIVGAGHAGGAVAANLRQFGWRGPITLIGEEPLPPY
                     QRPPLSKAWLKREADAASLLLRPAAFYASNDIALRLEQRVTGIDRTARQVRLATGSLV
                     PYDRLVLALGSVVRPLAVSGTELRGVLSLRNAADADRLQAALRPGARLAVIGGGYIGL
                     EVAASARALGAAAVVIEREDRVLARVAGPLLSDFFQRQHRARGVQLELGAVVETLEGD
                     GGAITGVRLRDGRLIPCDVALVGIGAVANDALAQAAGLQCGGGIVVDATGRSSDPAIY
                     AIGDCSNRPLPLYRRSVRLESVPNALEMAKQAAAALCGRPPPPSEVPWFWSDQFDLRL
                     QIAGLLFDAVQTAVRGDPDSGRFAVFHLAADDTVQAVEAVNAPAEFMAGKLMIARRRR
                     AAAEQLRDPACALQQLAA"
     gene            2136..2462
                     /locus_tag="VFN42_06130"
     CDS             2136..2462
                     /locus_tag="VFN42_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020043927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET8996228.1"
                     /translation="MPKVTYIEYNGTAHTVDVPVGTSVMRGAVDNNIPGIDADCGGEC
                     ACATCHVYVDAAWLEKTGLPVPGSQEASMLSFAAVTQPDSRLSCQISMTEALDGLVVR
                     MPEGQH"
     gene            2489..3736
                     /locus_tag="VFN42_06135"
     CDS             2489..3736
                     /locus_tag="VFN42_06135"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET8996229.1"
                     /translation="MNAPVGIDPNADAWTRPLTQFDMSDPSFYQDDTWHPYFARLRRE
                     APVHFTSDSRYGPYWSISRYKDIMQCEVNHAVFSNEIGGIQVEDQPKDMQRPSFIRMD
                     PPRHDEQRKVVSPIVAPANLANMEPLIRERTQRVLDGLPRGETFDWVERVSIELTTMM
                     LATLFDFPFEERRKLTHWSDVAIANVNAPDAPVHSEEERFAELKTMAEYMARLFAERR
                     EQPAKFDLLSMLAHGEATRDMPLREFMGNLALLIVGGNDTTRNSMTGGLLALNQFPDE
                     YRKLMDNPGLIESAVSEIIRFVTPVIHMRRTTTQEIEVAGHRIPAGAKVVMWYLSGNR
                     DEEAIEEPNRLTVDRKRPRQHLSFGFGIHRCVGNRLAELQLRILWEEILQRFPMIEVM
                     GPATRVYSNFIHGFRSMPVRIPA"
     gene            complement(3786..>4538)
                     /locus_tag="VFN42_06140"
     CDS             complement(3786..>4538)
                     /locus_tag="VFN42_06140"
                     /inference="COORDINATES: protein motif:HMM:NF015214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase C-terminal
                     domain-containing protein"
                     /protein_id="HET8996230.1"
                     /translation="FFCRDSVSLPRNDFDHPLSGRFDEQDAFVIFDNVEVPRHRVFID
                     GNLAVYNSVMSPSWRGNAMHQTMIRAWTKLEFAWGIATRMAETIGARDPVTQEMLGEI
                     WSYAELARSAVIAGEQQAYEYGNGAWFLDMRPMNALRATLPYWFPRVNEIIRLIGSHN
                     VLCTPNAEQFGDASLRPLIDKYLRGAGDVTAEQRARVFRLGWDFAGSALASRNEQYER
                     FYLTSGARNRQANQLRADRTDADRLVNRFLQA"
BASE COUNT          724 a         1528 c         1555 g          731 t
ORIGIN      
        1 cgcgcatggc gtattcgccc ggttccatca ccgtgccggc gcggccatag ccgtgcgatt
       61 ccaggaattc cgccagtttt tccggctgga ccgtgccgtt gacccgcagc tccatgctgg
      121 cgccggcgaa ggcggcgcgc ggcggcacgc gctgcaccag cgcgttgacc gtggtcagca
      181 cgatgcgcgg ccggtcgggt ttctccaaca gccgggtcag cgtggccagg cgttcggcca
      241 ccagcgcggc gttcggggag acgcggtcgt atggcaggca gtcccaggcc gggaagcgca
      301 ggatctcggc ttccggcagc acgaaggcca gcgactcggc cacgcgcgcc atgcgggcgt
      361 cgtcgcgggc gacgtgcagg acactgccct tgtgttcggc gcggcggcgg gccagcagga
      421 aagcatccca gccctcgggg gcaccccaga cctgagcggc ggcactcatc ggcccagcac
      481 gtcccggata cgcagcagca ttggcggcgc gtccgctggc atcggacggc ggccggtcag
      541 ccaatcagcc aggtcgggat caggcaattc cagcaactct tccagcgcgt cgagttcggg
      601 tggggcgagt ccgtccagat tggcgcggac aaaaccgccg agcatcaggt cgttctcgaa
      661 cgttccgcgg tgcgtggcgc ggtagagcag gcgacggcgc ctggtttcca gcgcggcgga
      721 gtcggggtct gtcatcaacg ctgtctcata tagcggtgcg gacgcagagg aaacaccccc
      781 ggcgccttcg tcttcgcgtg gtcacctacg ccgccctgcc tgcgcattcc gcttgaccgt
      841 ccgacccgcg ccatgcgcca atctactggc cagtagggag gtgcccgcgt ggacgccgat
      901 ccggcgcgga tcgtgattgt tggggcaggc cacgccgggg gcgcggtcgc ggccaacctg
      961 cgccaattcg gctggcgcgg cccgatcacc ctgatcggcg aggagccgct gccgccctac
     1021 cagcgcccgc cgctgtccaa ggcgtggctg aagcgcgagg cggatgccgc cagcctgctg
     1081 ctgcgccccg ccgccttcta cgcctccaac gacattgccc tgcggctgga gcagcgggtg
     1141 accggtatcg accggaccgc ccggcaggtg cggctcgcca ccggttccct cgtgccgtac
     1201 gaccgcctcg tcctggcgct gggctccgtg gtgcggcccc tggcagtgtc tggcacggag
     1261 ctccgcggcg tgctgtcgct gcgcaacgcc gccgatgccg accgtttgca ggccgccctg
     1321 cgccccggcg cccggctggc ggtgatcggc ggcggctata tcggcctgga ggtcgccgcc
     1381 tccgcccggg cgctgggagc cgcggcggtg gtgatcgagc gcgaagaccg cgtgctggcc
     1441 cgcgtcgccg gtccgctgct gtccgatttc ttccagcgcc agcatcgcgc ccgcggcgtg
     1501 cagctcgagt tgggagccgt ggtggagacg ctggaagggg acggtggcgc gatcaccggc
     1561 gtgcggctgc gcgatgggcg gctgatcccg tgcgacgtcg cgctggtcgg gatcggggcc
     1621 gtggcaaacg acgccctggc gcaggccgcc ggcctgcagt gcggcggcgg catcgtggtg
     1681 gatgcgacgg ggcgcagcag cgatccggcg atctacgcca tcggcgactg cagcaaccgc
     1741 ccgctgccgc tgtacaggcg ttccgttcgg ctagagagcg tgccgaacgc gctggagatg
     1801 gccaagcagg ccgctgccgc cttgtgcggc cggccgccac cgccgtccga agtgccctgg
     1861 ttctggtccg accagttcga tctgcgcctg cagatcgcgg gtctgctgtt tgacgcggtg
     1921 cagaccgccg tgcggggcga tcccgacagc ggccggtttg ccgtgttcca cctggccgcc
     1981 gacgacaccg tgcaggcggt ggaggcggtg aacgccccgg ctgaattcat ggccggcaaa
     2041 ctcatgatcg cacgccgccg tcgcgccgca gccgagcagt tgcgcgatcc ggcgtgcgca
     2101 ttacagcaac tcgcggcgta gccggaaggg aagtcatgcc gaaagtcacc tatatcgaat
     2161 ataatggcac cgcacacacg gtggacgtgc cggtcggcac ctccgtgatg cgcggggcgg
     2221 tggacaacaa catccccggc atcgatgccg attgcggcgg cgaatgtgcc tgcgcgacct
     2281 gtcacgtcta cgtggatgcc gcctggctgg agaagacagg attgccggtg ccgggcagcc
     2341 aggaagcgag catgctgagt ttcgccgcgg tcacgcagcc ggactcgcgc ctgtcctgcc
     2401 agatcagcat gaccgaggcg ctggacgggc tggtcgtgcg catgccggaa ggccaacact
     2461 gaagcaaacc gcaccaggga ggacgaccat gaacgcaccg gtgggaatcg atccgaacgc
     2521 agacgcctgg acgcgcccgc tgacacagtt cgacatgagt gacccgtcct tctaccagga
     2581 cgacacttgg cacccttact tcgctcggct gcggcgcgag gcgccggtgc atttcacctc
     2641 cgacagccgc tatgggccgt actggtcgat ctcccgctac aaggacatca tgcagtgcga
     2701 ggtcaaccat gccgtgttct ccaacgagat cggcggaatc caggtggaag accagccgaa
     2761 ggacatgcaa cgcccgtcct tcatccgcat ggatccaccg cggcatgacg agcagcgcaa
     2821 ggtggtcagc ccgatcgtcg cgccggccaa cctggccaac atggagccgc tgatccgcga
     2881 acgcacgcag cgcgtgctgg acggactgcc gcgtggcgag acgttcgact gggtggagcg
     2941 cgtgtcgatc gagttgacca ccatgatgct ggccacgctg ttcgattttc cgttcgagga
     3001 gcggcgcaag ctgacccact ggtcggacgt ggcgatcgcc aacgtgaacg cgccggacgc
     3061 gccggtgcac tccgaggagg agcgcttcgc cgagttgaag accatggcgg aatacatggc
     3121 ccgcttgttc gccgaacgcc gcgagcaacc ggcgaaattc gacctgctgt cgatgctggc
     3181 gcacggcgag gccacgcgcg atatgccgct gcgggaattc atgggcaatc tggcgctgct
     3241 gatcgtcggc ggcaacgata ccacccgcaa ctcaatgact ggtggcctgc tggcgctgaa
     3301 ccagttcccg gacgagtacc gcaagctgat ggacaatccc ggcctgatcg aaagcgcggt
     3361 ctcggagatc attcgtttcg tcacgccggt gatccatatg cggcggacca cgacgcagga
     3421 gatcgaggtt gcgggccacc gcatcccggc aggtgcgaag gtggtgatgt ggtacctgtc
     3481 cggcaatcgc gacgaggagg cgatcgaaga gccgaaccgg ctgaccgtcg atcgcaagcg
     3541 tccgcgccag cacctgtcat ttggcttcgg catccatcgc tgtgtcggca accggctggc
     3601 cgaactgcag ctgcgtatcc tgtgggagga gatcctgcag cggttcccga tgatcgaggt
     3661 gatgggaccg gcgacgcggg tctactccaa cttcattcac ggcttccgct ccatgccggt
     3721 gcggatcccg gcctgagcgg cgcctctcca tgtcatgacc ggcgcggccc gatgcccgca
     3781 gccggtcagg cttgcaggaa ccggttcacc agccggtccg catccgtccg gtcggcgcgc
     3841 agctggttgg cctgccggtt gcgtgcgccg gaggtcaggt agaaccgttc atattgctcg
     3901 ttccggctgg ccagcgcgct gccggcgaag tcccagccca ggcggaacac ccgcgctcgc
     3961 tgctccgcgg tcacatcgcc cgccccgcgc agatacttgt cgatcagcgg gcgcagcgag
     4021 gcatcaccga actgctccgc gttgggggtg cacagcacgt tatgtgagcc gatcagccgg
     4081 atgatctcgt tcacccgcgg aaaccagtat ggcagcgtcg cccgcagcgc gttcatcggc
     4141 cgcatgtcaa ggaaccacgc gccgttgccg tattcatagg cctgctgctc ccccgcgatc
     4201 accgccgagc gcgccagttc ggcatagctc cagatctcgc cgagcatttc ctgcgtgacc
     4261 ggatcgcgcg cgccgatggt ctccgccatg cgggtggcga tgccccaggc gaattccagc
     4321 ttcgtccatg cccgtatcat cgtctggtgc atcgcattgc cgcgccagct cggcgacatc
     4381 acgctgttgt agacggcaag gttgccgtca atgaagacgc ggtggcgcgg cacttccacg
     4441 ttgtcgaaga tcacgaatgc atcctgctcg tcgaaccggc cggacagcgg atgatcgaag
     4501 tcgttgcgcg gcagcgacac gctgtcgcgg cagaagaa
//
LOCUS       DASSZH010000286         2993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1438648, whole genome shotgun sequence.
ACCESSION   DASSZH010000286 DASSZH010000000
VERSION     DASSZH010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438648"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(263..478)
                     /locus_tag="VFN42_06145"
     CDS             complement(263..478)
                     /locus_tag="VFN42_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996231.1"
                     /translation="MNTRTMPAVSVLVPASWTPEQALAVLELLNDLRDALCAVHGDRI
                     RDMLQQEQGSTARDLQPSEPDDDNRSF"
     gene            complement(475..1227)
                     /gene="istB"
                     /locus_tag="VFN42_06150"
     CDS             complement(475..1227)
                     /gene="istB"
                     /locus_tag="VFN42_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HET8996232.1"
                     /translation="MSDPDLRRARAAALRLHGLLAHWPEVTAADWVVLLLGWEEQERH
                     RRSLERRLKEARIGRFKPLCDFDWSWPKRCDRAAFEALMALDFLKEAANVVLVGPNGV
                     GKSTLAQNIAHQALIEGHTVLFTSAGQLLGDLSALDSDSALRRRLRHYARPDLLVIDE
                     VGYLSYSNRHADLMFELVSRRYQNRSTLITTNRPFAEWCEVFPNAACVVSLIDRLVHN
                     AEIIAIDGESYRLKEARERTEQRARQRRGGKS"
     gene            complement(1220..2713)
                     /gene="istA"
                     /locus_tag="VFN42_06155"
     CDS             complement(1220..2713)
                     /gene="istA"
                     /locus_tag="VFN42_06155"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HET8996233.1"
                     /translation="MVTPPDIEAQVLRYYHAEKWTAGTIARQLHVHHSVVRRVLAQAG
                     LPRIGAAPRPSRIDAYLPLIRETLEKFPTLTASRLFAMVYERGYRGSPDHFRHLIALH
                     RPRRPAEAYLRLRSLPGEQAQVDWGHFGHLQIGRARRPLMAFVMVLSHSRQLFLRFFL
                     DARMESFLRGHVGAFTAWGGVPRVLLYDNLKSAVLERRGDAIRFHPSLLAFAGQYRFE
                     PRPVAVARGNEKGRVERAIGYVRTAFFAARDFTGLDDLNAQADAWCHGLAADRRCPGE
                     PNRTVRDVFADEAPRLLPLPDNPAPLLERVAVKLGKQPYVRFDLNDYSVPHTHVRRVL
                     TVLADTHEVRIVDGVHVLARHPRSYGKGEQVEQAEHVEALVAHKRAARQHRATDRLAQ
                     VAPASQDLLLRAAERGANLGAITTSLMRLLQRYGAAELQIGISEALDRGVPHPNAVRL
                     ALERRREQRGEPPPVSVDLPAAVQARDAPVRPHALDTYDRLKDVADE"
BASE COUNT          482 a          948 c         1089 g          474 t
ORIGIN      
        1 acgccatgtc acggtggaag gaccgtatct gccgcaccct gtcgatatga atgtgctggc
       61 tgagacggtc tgcgggttgt tgcctgtcac cggcggtgag gaagcgcgca tagacggcgg
      121 cgggcgtcgt ctgggggcgt gaccatgggg cgtgattgcc gagcccgaag caaaatgccg
      181 gtgatttcga agcgtggcat gtcggggtgg ccgcgagatc acggctgaga tggttggtgg
      241 agacgggcga cggcgctgct gctcagaacg atcggttgtc gtcgtccggt tcgctgggct
      301 gcaagtccct ggcggtggat ccctgttctt gttgcagcat gtcccggatg cggtcgccat
      361 ggacggcgca cagtgcatcc cgcaaatcgt tgagcaattc cagcacggcg agcgcctgct
      421 ctggcgtcca tgatgctggg acgagaacgg ataccgccgg catcgtgcgg gtgttcatga
      481 tttgccgccg cggcgctgac gggcgcgttg ctcggtgcgt tcgcgcgcct ccttgaggcg
      541 gtaggattcg ccgtcgatgg caatgatctc ggcgttatgc accagccgat cgatcagcga
      601 gacaacgcaa gcggcgttgg ggaagacctc gcaccactcg gcgaacgggc ggttggtggt
      661 gatcagcgtg ctgcggtttt ggtagcggcg ggagaccagt tcgaacatca ggtctgcgtg
      721 acggttggaa taggagaggt agccgacctc gtcgatcacc agcaggtccg gtctggcgta
      781 gtggcgcagg cggcggcgca aggcggaatc gctgtcgagc gcgctgaggt cgccgagcaa
      841 ttgtccggcg gaggtgaaca acacggtgtg gccctcgatc agcgcctggt gggcgatgtt
      901 ctgtgccagg gtggacttgc cgacgccgtt gggaccgacc aggacgacgt tggcggcctc
      961 cttgaggaag tcgagcgcca tcagggcctc gaaggcggca cggtcgcagc gctttggcca
     1021 gctccagtcg aagtcgcaga gcggcttgaa gcggccgatc cgcgcctcct tcaggcgccg
     1081 ctccaggctg cggcggtgac gctcctgctc ctcccagccc agcagtaaca cgacccagtc
     1141 ggccgcggtg acctccggcc aatgcgccag caatccatgc agacgcagcg ccgcagcgcg
     1201 ggcgcgccgg agatcggggt cactcatcgg caacgtcctt gagccggtcg taggtatcga
     1261 gcgcatgcgg tcgcactggc gcatcgcgtg cctgcaccgc ggcgggcaga tcaaccgaga
     1321 ccggcggcgg ttcgccgcgt tgctcgcggc ggcgttccag cgcgaggcgg acggcattgg
     1381 gatgcggcac gccgcggtcg agcgcctcgc ttatgccgat ctgcaactcg gcggcgccat
     1441 atcgctgcag caatcgcatc aggctggtgg tgatggcgcc gaggttggcg ccgcgttcgg
     1501 cggcgcgcag cagcaggtcc tgactggccg gagcgacctg ggcgaggcga tcagtggcgc
     1561 gatgctggcg ggcagcgcgt ttgtgcgcga ccagggcctc gacgtgttca gcctgctcga
     1621 cctgctcccc cttgccatag ctgcggggat ggcgggccag tacatgcacg ccatcgacaa
     1681 tgcggacctc atgggtgtcg gccagcaccg tcagcacccg ccgtacgtga gtgtgcggca
     1741 cggaataatc gttcaggtcg aagcggacgt agggttgctt gccgagcttg acggcgacgc
     1801 gttcgagcaa cggcgccggg ttgtcgggca gcggtaacag gcgcggcgcc tcgtcggcaa
     1861 atacgtcccg caccgtgcgg ttgggttcac cgggacagcg ccggtcggca gccaggccgt
     1921 ggcaccaggc gtcggcctgg gcgttgagat cgtccagacc cgtgaagtca cgcgcggcga
     1981 agaacgcggt gcgcacgtag ccaatggccc gttcgacccg gcccttctcg ttgccgcggg
     2041 ccaccgcgac cggccgcggc tcgaagcgat actgcccggc aaaggcgagc aagctcgggt
     2101 gaaagcggat cgcatcgccg cgccgttcca gcaccgcact cttgagattg tcgtagagca
     2161 gcacacgcgg cacaccgccc caggcagtga acgcgccgac atggccgcgc aggaaactct
     2221 ccatccgcgc gtccagaaag aagcgcagga agagctgccg cgagtggctc agcaccatga
     2281 cgaacgccat cagcgggcgc cgggcccggc cgatttgcag gtggccgaaa tgcccccagt
     2341 ccacttgcgc ttgctcgccc ggcaggctgc gcagccgcag atacgcctcg gccggacggc
     2401 gcggccggtg cagcgcaatc aggtggcgga agtgatccgg gctgccgcgg tagccgcgct
     2461 catacaccat cgcgaacagg cggctcgcgg tcagggtggg gaatttctcc agcgtctcgc
     2521 ggatcagcgg cagataggcg tcgatccgtg acggccgtgg cgcggcaccg atccggggga
     2581 gcccggcctg cgccagcacc cggcgcacca cgctgtggtg cacgtgcaac tgacgggcga
     2641 tcgtgccggc ggtccacttc tcggcgtgat agtagcgcag gacctgcgct tcgatgtcag
     2701 gcggcgtcac catggcgcat tggccccgtt gggtggtgtc caaggcggcc gcgatgacgc
     2761 cggcggcggc gcaggaaccc ctgacggagc catggcaggc agcggcggcc gcaccagtgg
     2821 cagtgcaggc caggcaacca ccccccatcg gcaggggaag cgtcatcgcg cgcgatcggc
     2881 atcgcgtccg gtgccacaag agcaccagcc gatggcgcgg gggaaccgtg atgcgtcact
     2941 ctttctcgcc gcgtccgata acggctcatc ctggcggcgt gggcgcgccg gcc
//
LOCUS       DASSZH010000287         3350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_909843, whole genome shotgun sequence.
ACCESSION   DASSZH010000287 DASSZH010000000
VERSION     DASSZH010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909843"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1261
                     /locus_tag="VFN42_06160"
     CDS             77..1261
                     /locus_tag="VFN42_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996234.1"
                     /translation="MSERTLRGKAAVVGIGQTTYYKHGGSPDSEFKLALMAVVNACKD
                     AGISPHEVDGFASYGNDRSDAPRLASALGCKEFRFSNMFWGGGGGGVCGAVANAAAAV
                     ATGMADCVVAFRSLAQGQFARYGRGTGAPLAAGDDQFLFPYGMMSPAQRFAMKVTRFM
                     HEHGIRQEALRAISIACYHHAQNNPNAVMRGRPLDEAKYDNSRWIIEPFFHLFDCCQE
                     NDGAAAVIVMSADRAKDFPNKPAYMLGAVQGCDHRSAAPVHNMPGYPSSHFRALAPHL
                     YNMAKLGPADMQVVQCYENFTGGVLMSLVEHGLVKPDEANEFLVTENLLAPDGRMPLN
                     TSGGNLAECYMHGLELVIEAVHQIRGDAVNQLKRNDTAIIIGGPMVTPVSSIILGSEA
                     AL"
     gene            1258..1692
                     /locus_tag="VFN42_06165"
     CDS             1258..1692
                     /locus_tag="VFN42_06165"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HET8996235.1"
                     /translation="MSDFYLPEGLPAPSSAPDGLAQPYWDGTKQRKLMVQKCRGCGAW
                     QWGPEWICHRCHSFDLGWQEIEGCGRIYSYQRPHHPVHAALNGHGPYIVVLVELPQYG
                     NIRMVGNLLGDPRQAVKIGAPVEAVFEPHADFTLVQWRVSGD"
     gene            complement(1689..2576)
                     /locus_tag="VFN42_06170"
     CDS             complement(1689..2576)
                     /locus_tag="VFN42_06170"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET8996236.1"
                     /translation="MTAPGDIRIGISGWNYPGWRGVFYPKGLPQKRELEYASRQFRTI
                     EVNGTFYRAQRPEYFAAWVEQTPDDFVFAVKGPRYITHMLRLTKSETPLANFFATGVL
                     RLGAKLGPILWQFPPQFQFHPERIEPFLQMLPRDTEAAAELAHRHDSRFSGRTWLRTD
                     ARRVLRHAFEIRHESFRSPAFIALLRRYNVALVCADTVEWPLLMDVTADFVYCRLHGS
                     EELYASGYDDAALDTWAARVAAWARGSEPCDENRVAGKAHRRHRDVFVYFDNDAKVRA
                     PQDAQGLVQRVSALVGKPG"
     gene            complement(2573..>3350)
                     /locus_tag="VFN42_06175"
     CDS             complement(2573..>3350)
                     /locus_tag="VFN42_06175"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HET8996237.1"
                     /translation="FPLSWTLDHCGPLSRSVEDSAITLQVLAGHDPQDPASAAVPVPD
                     YRAGLERGVTGLRIGIPRAFFAKAQATMPEVAAGVDRTAAQLRDAGAVVEDVILPEYA
                     LFAATGRIIMMAEAFAIHQADMQTRLLDYGEITASRFVLGAAITAADFINALRARREL
                     TEAVNAALSRYDALLTVSALNTAPRFDEPVDTMSSAAPMQTIPFNVTGHPAMSVPVGL
                     ANGLPIGVQVVGRPFDEATVFRVGRAIELLSGWESVALPV"
BASE COUNT          600 a         1123 c         1087 g          540 t
ORIGIN      
        1 agattcccaa agcacacccc gccggatccc ccgcaaccat tcgtggatgg caggcctgcg
       61 cccgccatga cgtggcatga gcgaacgtac tctccgcggc aaagccgctg tcgtcggcat
      121 cggccagaca acctactaca agcatggcgg ctcgccggat tccgagttca agctggcgct
      181 gatggcggtg gtgaacgcct gcaaggacgc cggcatcagc ccgcacgaag tggatggctt
      241 cgcctcctac ggcaacgacc gcagcgatgc gccgcgtctt gcctccgctc tcggctgcaa
      301 ggaattccgc ttctccaaca tgttctgggg cggtggcggt ggcggcgtgt gcggcgcggt
      361 ggcgaatgcc gccgcagcgg tggcaaccgg catggcggat tgcgtggtgg cgttccgctc
      421 gctggcacag ggccagttcg cgcgctacgg ccgcggcacc ggcgcgccgc ttgccgcggg
      481 cgacgaccag ttcctgttcc cctacggcat gatgtccccg gcgcagcgct tcgccatgaa
      541 ggtgacgcgc ttcatgcacg agcacggcat ccggcaggaa gcgctgcggg cgatctcgat
      601 cgcatgctat caccacgcgc agaacaatcc caacgcggtg atgcgcggcc ggccactgga
      661 cgaggcgaaa tacgacaact cccgctggat catcgagccg ttcttccacc tgttcgactg
      721 ctgccaggag aatgatggcg ccgccgcggt gatcgtcatg tcggccgacc gggcgaagga
      781 cttccccaac aagcccgcct acatgctggg cgccgtgcaa ggctgcgacc accgcagcgc
      841 cgcgccggta cacaacatgc ccggctatcc cagctcgcat ttccgtgccc tggcgcctca
      901 cttgtacaac atggcaaagc tgggtcctgc ggatatgcag gtggtgcaat gctatgagaa
      961 cttcaccggc ggcgtgctga tgagcctggt ggagcacggg ctggtcaagc cggacgaagc
     1021 caacgagttt ctggtgacgg aaaacctact ggcgcccgac ggccgcatgc cattgaacac
     1081 cagcggcggc aatctggcgg aatgctacat gcacgggctg gaactggtga tcgaagccgt
     1141 gcaccagatc cgcggcgacg cggtcaatca gttgaagcgc aacgacaccg ccatcatcat
     1201 cggcggcccg atggtgacgc cggtgagttc catcattctt ggttcggagg ccgcgctgtg
     1261 agcgacttct atctgcccga aggcctcccc gccccgtcca gcgcgccgga tggactggcc
     1321 cagccgtact gggacggcac gaagcagcgc aagctgatgg tgcagaaatg ccgcggttgc
     1381 ggtgcgtggc agtggggacc ggaatggatc tgccaccgct gccacagctt cgacctgggc
     1441 tggcaggaaa tcgaagggtg cggccgcatc tactcctatc agcgcccgca tcacccggtg
     1501 cacgcggcac tcaatggcca cgggccgtat atcgtggtgc tggtggagct gccgcagtac
     1561 ggcaacatcc gcatggtcgg caacctgctg ggcgatccgc gtcaggcggt gaagatcggt
     1621 gcgccggtgg aggcggtgtt cgagccgcat gccgacttca cgctggtgca gtggcgcgtc
     1681 agcggcgatt aacccggctt gccgaccagc gccgagacgc gctgcaccaa tccctgcgcg
     1741 tcctggggtg cccgtacctt ggcgtcgttg tcgaaataga caaagacgtc acggtgcctg
     1801 cggtgcgcct tgccggcaac acggttttcg tcgcacggtt cgctgccccg cgcccaggcc
     1861 gccacgcggg ccgcccaggt atccagcgcc gcatcatcgt agccgctggc gtatagctcc
     1921 tccgagccat gcagccggca gtagacaaaa tccgcggtga catccatgag cagcggccat
     1981 tccacggtat ccgcacatac cagcgcgacg ttatagcggc gcagcaacgc gatgaacgcg
     2041 ggcgagcgga agctctcgtg gcggatctcg aacgcgtggc gcagcacgcg cctggcatcg
     2101 gtccgcagcc aagtgcggcc ggagaaccgg ctgtcgtgac gatgcgccag ttcggcggca
     2161 gcctcggtat cgcgcggcag catctgtagg aatggctcga tccgctccgg gtggaactgg
     2221 aactgcggcg gaaactgcca caggatcggc ccgagcttcg cgcccagtcg caacaccccg
     2281 gtggcaaaga aattggccag cggcgtctcc gattttgtca gccgtagcat gtgcgtgatg
     2341 tagcgcggcc ccttcaccgc gaacacaaaa tcgtccggag tctgctcaac ccatgccgca
     2401 aaatactccg gccgctgcgc gcggtagaac gtgccgttga cctcgatcgt gcgaaactga
     2461 cgggacgcgt attccagttc gcgcttctgc ggcagcccct tcgggtagaa caccccacgc
     2521 cagccgggat agttccagcc ggagatgcca atgcggatgt cgccgggggc ggtcacaccg
     2581 gcaaagccac actctcccag ccggacagaa gctcgatcgc gcggccgacg cggaacaccg
     2641 ttgcctcatc aaaggggcgg ccgaccacct gcacgccgat cggcaggccg ttcgccagcc
     2701 ccaccggcac actcatcgcc ggatggcccg tgacgttgaa tgggatggtc tgcattggcg
     2761 cggcactgga catggtgtcc accggctcat caaagcgtgg cgcggtgttc agtgccgata
     2821 cggtcagcag tgcatcatag cgtgacagcg ccgcattcac cgcttcggtc agctcccgcc
     2881 gggcacgcag cgcgttgatg aagtcggccg cggtgatcgc cgcgccgagc acaaagcggc
     2941 tggcggtgat ctcgccatag tccagcaagc gggtctgcat gtcggcctgg tgaatggcga
     3001 acgcctccgc catcatgatg atccgcccgg tcgcggcgaa cagcgcgtat tccggcagga
     3061 tcacgtcttc caccacggca cctgcgtcgc gcagttgcgc cgcggtgcga tcgacgccgg
     3121 cggccacttc cggcatggtc gcctgcgcct tggcgaagaa cgcgcgtggg ataccgatgc
     3181 gcaagcccgt cacgccccgc tccagtcccg cgcgataatc cggcaccggc acggcggcgc
     3241 tggctgggtc ctgcggatcg tgccccgcca gaacctgcag cgtgatcgcg gaatcctcca
     3301 cgctgcggct caaaggtccg cagtggtcca gcgtccagga caacggaaac
//
LOCUS       DASSZH010000288         2614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1217076, whole genome shotgun sequence.
ACCESSION   DASSZH010000288 DASSZH010000000
VERSION     DASSZH010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217076"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VFN42_06180"
     CDS             <1..434
                     /locus_tag="VFN42_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996238.1"
                     /translation="DAISGQLADLNKQIDKLNSDEMQLHKLERSRAILEDDYKAVSKI
                     LDEREIVESVEAHRESSVRVIQPPRAPDLPLPTRRLILMAGIVVSMLLSIGSVLMAHF
                     FRAIYLRPEALEMDTGLTVLASVPEMRAIGGPSSGVLVVPG"
     gene            446..1459
                     /locus_tag="VFN42_06185"
     CDS             446..1459
                     /locus_tag="VFN42_06185"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8996239.1"
                     /translation="MDTRTPVLVLGASGFIGSHVVAALSSHPAYRPIAASRKAAADGS
                     RVALDATNPDAVRAAVREVGAVVNCLAGGEGAMTRATTALCEANPARIVHLSSMAVYG
                     AATGTVREDHTPVQPVGGYGQAKIDAEAIVRRYADAGGQAVILRPTCVFGPGSSQWTT
                     RIARLLQARRLGDLGSAGDGGCNLAFIDDLVDTVIASLDAPSGGVFNISSSAELTWNQ
                     FLLRFARALDATPVRRIPARMLRLETKLLAPARRIGSKLVKSPVTEAITPSLAALFAQ
                     DIWIDCSAATAELGLRPTPLDRMIDTSVRWLGPRQPGAPDARPRTRVARPGAAVTAGQ
                     AGR"
     gene            1459..2586
                     /locus_tag="VFN42_06190"
     CDS             1459..2586
                     /locus_tag="VFN42_06190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET8996240.1"
                     /translation="MKNRRTVFLAAPYGQVGGGMGSIMAYLASRQHDPSGRFDLKQLE
                     TRGGGHVALSPIFLTAAIGRIALEAARGRLAIVHLNLAERGSVYRKAALLAATKLAGG
                     RVLLHLHAAQIRQFYDAMDSRGKALVRWVFQSADHIVVLGEVWRHWVTTTFGVDPQRV
                     SIVYNGVPATVAKARTVPDDGRFRLLFVGNLMERKGVSDLLRAFAKPSIRARNAHLTM
                     AGGGPVDKYRAMAAELGIADRVTFTGWVSQDDARTLMVNADALILPAYDEGLPLVILE
                     ALATGTPVICTPVGSIPEVLADRHNALFVTPGDEAAIAEAILALIDDPAMGRALATEG
                     SALYQRLFTMDAFARAIGTFYSALIPAGNEPEGSVPATANH"
BASE COUNT          402 a          843 c          948 g          421 t
ORIGIN      
        1 acgacgcgat ctcgggtcag ctggccgacc tgaacaagca gatcgacaag ctcaacagcg
       61 acgaaatgca gctgcacaag ctggaacgca gccgcgccat cctggaagat gactacaagg
      121 ccgtgtcgaa gatcctggat gagcgggaaa tcgtcgagtc cgtcgaagcg catcgtgaat
      181 cctcggtgcg cgtgatccag ccgccgcggg cacctgacct gccgctgccg acccgccgcc
      241 tgatcctgat ggccggcatc gtcgtcagca tgctgctgtc gatcgggtcg gtgctgatgg
      301 cgcacttctt ccgggcgatc tacctgcggc cggaggcgct ggagatggat accggcctga
      361 cggtgctggc atcggtgccg gagatgcggg ccatcggcgg cccgtcgtcc ggtgtgctgg
      421 tggtcccggg ttgagcggga ccatcatgga cacgcgcaca ccggttctgg tgctgggggc
      481 gtcgggcttc atcggctccc acgttgtggc cgcattgtcc tcgcatcccg cctaccggcc
      541 gatcgcggcg tcccgcaagg cggccgcgga cgggtcccgg gtggcgctgg acgcgaccaa
      601 cccggacgct gtgcgggcgg cggtgcgcga agtcggtgcc gtggtgaact gccttgccgg
      661 tggtgagggc gcgatgaccc gcgccaccac ggcgctgtgc gaggcaaacc cggcccgcat
      721 cgtgcacctg agcagcatgg ccgtatacgg tgcggcgacc ggcacggtgc gggaggatca
      781 cacaccggtg cagccggttg gcggctacgg gcaggccaag atcgacgccg aggcgatcgt
      841 ccggcgctat gccgacgccg gcggacaggc cgtcatcctg cggccgacct gtgtgttcgg
      901 ccctggcagc agccaatgga cgacgcgtat cgcccggctg ctgcaggcgc ggcggctggg
      961 cgacctgggc agcgccggcg acggcggctg caatctggcc ttcatcgatg acctggtgga
     1021 cacggtcatc gcgtcactgg atgccccctc cggcggggtg ttcaacatct ccagctccgc
     1081 cgaactgacc tggaaccagt tcctgctgcg gttcgcccgg gcgctggacg cgacgccagt
     1141 gcggcgcatc ccggcgcgga tgctgcggct ggaaacgaag ctgctggcgc cggcacgccg
     1201 gatcggttcg aaactggtga aatcgccggt aaccgaggcg attacaccgt ccctcgccgc
     1261 gctgttcgcg caggacatct ggatcgattg ctccgcggcg accgccgagc tggggctgcg
     1321 gccgacgccg ctggacagga tgatcgatac gtcggtgcgc tggcttggac cgaggcagcc
     1381 cggcgcgccg gatgcaaggc cccgcacgcg ggtggcgcgg ccgggtgccg ccgtgacggc
     1441 gggccaggca ggccggtgat gaaaaaccgg cgcaccgtgt tcctcgcggc accgtacggc
     1501 caggttggcg gcgggatggg cagcatcatg gcctatctgg cgtcgcggca gcatgacccg
     1561 tcgggacgct ttgacctgaa gcagctggaa acacggggcg gcgggcacgt cgcgttgtcc
     1621 cccatctttc tgacggccgc catcgggcgc atcgccctgg aggccgcacg cggccggctg
     1681 gcgatcgtgc acctgaacct ggcggaacgc ggcagcgtgt atcgcaaggc ggcgttgctg
     1741 gcggccacga agctggcggg cgggcgggtg ctgctgcacc tgcacgcggc gcagatccgc
     1801 cagttttatg atgcgatgga ttcccgcggc aaggcgctgg tgcgctgggt gttccagtca
     1861 gcggaccaca tcgtggtgct gggcgaagtg tggcggcact gggtgacgac gacattcggc
     1921 gtcgatcccc agcgtgtcag cattgtttac aacggtgtgc cagcgaccgt ggcgaaggcc
     1981 cggaccgtgc cggacgacgg ccgcttccgg ctgctgttcg tcggtaacct gatggaacgc
     2041 aagggtgtgt cggacctgct gcgagccttc gcgaagccga gcatccgggc tcgcaacgct
     2101 cacttgacca tggcgggtgg cggaccggtg gacaaatatc gcgcgatggc ggcggagctt
     2161 ggcatcgccg accgcgttac ctttaccggc tgggtcagcc aggacgatgc gcggacgctg
     2221 atggtcaatg ccgatgcgct catcctgcca gcctacgacg aaggcctgcc gctggtgatc
     2281 ctggaggcgc tggcaaccgg aaccccggtg atctgcaccc ctgtcgggtc aatcccggag
     2341 gtgctggcgg atcggcacaa cgcgctgttc gtcaccccgg gggatgaggc cgcgattgcc
     2401 gaggcgatcc tggccctgat cgacgatccg gcgatgggcc gggcgctggc gacggagggg
     2461 agcgctctct accagcggct tttcacgatg gatgcgttcg cccgcgccat cggcactttc
     2521 tattcggcgc tgattccggc gggcaacgaa ccggagggct cggtgccggc cactgccaac
     2581 cactgaaagc gaacgatgat tctgcacgcc gatg
//
LOCUS       DASSZH010000289         6911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_450027, whole genome shotgun sequence.
ACCESSION   DASSZH010000289 DASSZH010000000
VERSION     DASSZH010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6911
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450027"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="VFN42_06195"
     CDS             complement(<1..1109)
                     /locus_tag="VFN42_06195"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET8996241.1"
                     /translation="MLIRRLGGALFAAFLVLAGLMPAHAQGDKKPITIGFGMALTGGL
                     APNGKAALLAMQMWEHDINAKGGLLGRPVKLVYYDDQSNPATVPGIYTKLLDVDHVDL
                     VVSGYATNMIAPAMPIIMQHHRTFLSLFGLAANSKFHYDRYFSFVPTGGPHPKESFSH
                     GFFAVAMHLNPKPKTIALVGADAEFALNGLDGARAWVKHYGLKTVYDRTYPPSTADFT
                     PIVRAIKATHPDLVMSFSYPPDAVGMVRAVHEVGLKTQLFGGGLVGLQATAIKTQLGP
                     LLNGIVNYDFWLPWTKLASKDSLAFLKQYQAKAPAAGVDALGYYMGPFAYGYLQVLQQ
                     AVEGTKSLDQAKLAEYLRSHTFNTVAGPVKFGPNG"
     gene            1235..2137
                     /locus_tag="VFN42_06200"
     CDS             1235..2137
                     /locus_tag="VFN42_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HET8996242.1"
                     /translation="MRDLDEHTVTDAALQQMAGTSDARLKQIMDAAVRHLHAFVREVE
                     LTPAEWLAGIGFLTAVGQACTPVRQEFILLSDILGVSAAVNAIHDRKARELGSQSSLL
                     GPFYREGAPELKLGDCIVQQPSVEEIVLYGRVTDNSGTPLPDALIQVWQTSEHGLYDL
                     QVQGGAEMDMRANFRTDAEGRYWFRTVRPLGYSIPMDGPVGDLVKRQNRHGFRPSHIH
                     FLIGAPGYRELVTALYFGDDEHIDSDTVFGVSGSLVVAAQQDPNAPIPGLRAVHYDFR
                     LARAAANETGRVGSDPSRLMPAAE"
     gene            2142..2930
                     /locus_tag="VFN42_06205"
     CDS             2142..2930
                     /locus_tag="VFN42_06205"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET8996243.1"
                     /translation="MTRRLIDISSPLRAGIKSDPPHMLPQIDYMDHHATAGQMAEYMG
                     VSVDQLPDGEYAAVERVTISTHNGTHLDAPYHFFSTMNHALKPGGEPSWRIDEVPLEW
                     CFQPGVKLDFRHFADGYVATPGDVEAELKRIGHTLRSLEIVVVNTSAGAKYGQDDYVD
                     SGCGIGKAATLWLLERGVRVTGTDGWSWDAPFSHTRRKVAATGDAGLIWEGHRAGREI
                     GYCHMEKLHDLEQLPADGFEIACFPVKVHRASAGWTRAVAILPG"
     gene            3254..4498
                     /locus_tag="VFN42_06210"
     CDS             3254..4498
                     /locus_tag="VFN42_06210"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HET8996244.1"
                     /translation="MDTEPLVFVGIDWASTEHQVCLMGAGEPIQRAFAHDAAGIGAMV
                     DWLCGQAKQSGQVAVAIETPHGPVVEALMDRGIAVFAINPKQLDRFRDRFSPAGAKDD
                     RRDALVLASSLRTDRHCFRRVDALDPAVVELREWSRMAEELKGERVRLANRVRQQLWR
                     YYPQALDLTDDLGADWVLALWAKAPTPADARRLTEKKVARVLAAHRIRRITAADALAI
                     LQRPALTVAPGTAEAARAHITATAERLYLVNRQIKDVTRRLDALVEQLAGPEPEPGQA
                     AEQRDATILRSLPGVGRIVLATLLAEAHQAIQARDYHALRTLAGVAPVTKRSGKSRRV
                     EMRQACSNRLRTAVYHWARVATQHDVRSRSRYAALRARGHSHGRALRTVADRLLGIAC
                     IMLTNRTTYEPTHEATRAPKTT"
     gene            4590..5402
                     /gene="thiD"
                     /locus_tag="VFN42_06215"
     CDS             4590..5402
                     /gene="thiD"
                     /locus_tag="VFN42_06215"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003624487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET8996245.1"
                     /translation="MIPNVLSIAGSDPSGGAGIQADLKSFAAMGAYGMAAITTLTAQN
                     TQGVRGFVAMEPDFVTAQIDAVFDDIRVDAVKIGMVATAPIARAIAAALRRHKPDNVV
                     LDPVMVATSGDRLLRDDAVAAIRDELAPLATVITPNMPEAAVLIGSGGPDSLAAMRAM
                     LPTLHRLGCEWVLLKAGHLDSDDRTDLLFDGTTVTELPGRRIDTRNTHGTGCSLSSAI
                     AALLPRHPVPEAARRAKAWLTGALAGSDQLDVGSGHGPVHHFHALWPKEPAA"
     gene            5399..6070
                     /gene="tenA"
                     /locus_tag="VFN42_06220"
     CDS             5399..6070
                     /gene="tenA"
                     /locus_tag="VFN42_06220"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HET8996246.1"
                     /translation="MTGFSEAAWQRVAPLRAAIHALPFNTELAAGTLSSARFQGYIIQ
                     DALYLTQYSRVLALAGARAPDGAALHAFASSALEAVAVEQALHERYMAQFGVDPARLA
                     TAEPSPDCLGYTSFLLACAYHEPWEILLAALLPCFWIYWDVGTAVARRAAADNPYRAW
                     IDTYADEAFGEAVRTVIAITDRAAAAMTDAVRDRMMRAFIRSSQFEWLFWDGAYQQRG
                     WPVGL"
     gene            complement(6226..>6911)
                     /locus_tag="VFN42_06225"
     CDS             complement(6226..>6911)
                     /locus_tag="VFN42_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET8996247.1"
                     /translation="EKPGGYSKFPWYALRQNYLDAIDAAGGLPVALPHEPVLAEAFLD
                     RIDALVVTGGAFDVDPALYGVGDRHATVTLKEGRTAAELALTRGALERTLPVLGICGG
                     QQLLAVALGGTLIQHIPDTIPEALEHEQPNPRHEPGHAVAVTRGTLLHRIVGATEMRV
                     NSAHHQAVRDPGPHAVVNAVAPDTVIEGIEDTRYRFCLGVQWHPEFFIDPGDRRIFDA
                     LVAACDARG"
BASE COUNT         1145 a         2365 c         2284 g         1117 t
ORIGIN      
        1 tcgccattcg ggccgaactt caccgggccg gccacggtgt tgaaggtgtg gctccgcagg
       61 tactcggcca gcttggcctg gtcgagactc ttggtgcctt cgactgcctg ttgcagcacc
      121 tgcagatagc cataggcaaa cggacccatg tagtagccca gcgcatccac gccggcagcc
      181 ggtgccttcg cctggtactg cttcaggaat gccagcgagt ccttgctcgc cagcttggtc
      241 cagggcaacc agaaatcgta gttcacaatg ccgttcagca acggtcccaa ctgggtcttg
      301 atcgctgtcg cctgcaatcc gaccaggccg ccaccgaaca gttgggtctt taacccgact
      361 tcatgcaccg cccggaccat gccgacggcg tccggtggat aggagaatga catcaccaga
      421 tcgggatgcg tcgccttgat ggcgcggacg atcggggtga aatccgccgt ggatggcgga
      481 taggtccggt cgtacaccgt cttcaggcca tagtgcttga cccaggcccg tgctccatcg
      541 aggccattga gcgcgaactc ggcatccgca ccaaccagcg cgatggtctt cggcttcgga
      601 ttcaagtgca tcgccacggc gaagaacccg tgcgagaagc tctccttcgg atgcggtccg
      661 ccggtcggca caaaggagaa atagcggtcg tagtggaact tgctgttcgc cgcgaggccg
      721 aacaggctga gaaaggtgcg gtgatgctgc atgatgatcg gcatggccgg cgcgatcatg
      781 ttggtggcat agccgctgac caccaggtcc acgtggtcca cgtccagcag cttggtgtag
      841 atgccgggta ccgtcgccgg gtttgactga tcgtcataat agaccagctt caccgggcgg
      901 ccgagcagcc cgcccttggc gttgatgtcg tgttcccaca tctgcatggc gagcagcgcg
      961 gccttgccgt tcggcgccag accgccggtc agcgccatgc cgaagccgat ggtgatgggc
     1021 ttcttgtcgc cctgtgcgtg cgccggcatc aggccggcca gcacaaggaa agcggcaaac
     1081 agtgcgccgc ccaggcgcct gatcaacatc cgtacgttcc tccccattgg tggttgacgc
     1141 gcatcctatg gcgcgaatgc ccgcgctgcc aagcctcgcc acgcagcctg ggatcggctt
     1201 atgctggccg ccagcaagtc caggagagca ggcaatgcga gaccttgacg aacacaccgt
     1261 caccgatgcc gcgctgcagc agatggcagg caccagcgat gcccggctga agcagatcat
     1321 ggacgccgcg gtgcggcacc tgcacgcctt cgtccgcgag gtggaactca ccccggcgga
     1381 atggctcgcc ggcatcggct tcctcactgc tgtcggccag gcctgcacgc cggtgcggca
     1441 ggagttcatc ctgctgtccg acatcctggg cgtcagcgcc gcggtgaatg cgatccacga
     1501 ccgcaaggcg cgcgaacttg gctcgcagag cagcctgctg ggtccattct atcgcgaagg
     1561 cgcgccggag ctgaagctgg gagactgcat cgtgcagcag ccctcggtgg aggagattgt
     1621 gctgtacggc cgcgttaccg ataacagcgg cactccgctg ccggatgcgc tgatccaggt
     1681 gtggcagacc agcgagcacg ggctgtacga cctgcaggtg caaggcggcg cggagatgga
     1741 catgcgcgcc aacttccgca ccgacgccga gggccgctac tggttccgca ccgtgcgtcc
     1801 gctcggctac tccattccta tggacggacc ggtcggcgac ctggtgaagc ggcagaaccg
     1861 ccatggcttc cgcccgtcgc acatccactt cctgatcggc gcgcccggtt accgcgagct
     1921 ggtcacggcg ctgtactttg gcgacgacga acacatcgat tccgatacgg tgttcggcgt
     1981 gtccggttcg ctggtggtgg cggcgcagca ggacccgaac gcgccgatcc ccgggctgcg
     2041 cgcggtgcac tacgatttcc gcttggcccg cgcggcggcg aacgagaccg gccgcgtcgg
     2101 ctccgatccg tcacgcctga tgccggcggc ggagtaaagc gatgacgcgc cgcctgatcg
     2161 atatctcctc gccgttgcgc gcggggatca agagcgatcc gccgcatatg ctgccgcaga
     2221 tcgactacat ggaccaccat gccaccgccg ggcagatggc ggaatacatg ggcgtcagcg
     2281 tggaccagtt gccggatggc gaatatgcgg cggtggaacg cgtcaccatc agcacgcata
     2341 acggcacgca tctggacgcg ccgtaccact tcttctccac gatgaaccat gcgctgaaac
     2401 cgggcggcga accgtcctgg cggatcgacg aagtgccgct ggaatggtgc ttccagcccg
     2461 gggtgaagct ggacttccgt cacttcgcgg acggctacgt ggcgacgccc ggcgatgtgg
     2521 aggcggagct gaagcgcatc ggccatacgc tgcggtcgct ggaaatcgtg gtggtgaaca
     2581 ccagcgccgg cgcgaaatac ggccaggacg actacgtgga ttccggctgc ggcatcggca
     2641 aggcggcgac gctttggctg ctggaacgcg gggtgcgcgt cacgggaacg gacggctgga
     2701 gctgggacgc gccattctcc cacacccgtc gcaaggtggc ggcgaccggc gatgcagggc
     2761 tgatctggga aggccaccgc gccgggcgcg agatcggcta ctgccacatg gagaaactgc
     2821 acgacctgga gcaattgccg gcggatggct tcgagatcgc ctgcttcccg gtgaaggtgc
     2881 accgcgcctc ggccggctgg acgcgagccg tggcgattct gccggggtag aacagcggtc
     2941 acgaattccc cctccccttg cgggaggggg tagggggagg ggcgtgcatg agttcccggg
     3001 agtgtcccaa cgacccctgt acctgtctgc ctgcggttta tgatgcatca tagccgtgtc
     3061 gggccgatcg gttgagagca gcctttcgtc ccctgtgccg tcgagcacct ggggaggacg
     3121 cgcgcctcag ccggtcgccc gtgttgcacg cccagtgggg agtcgcgcct cggctcattg
     3181 cagccgcgag cgccagtaca gggaacggcg aagcgacgcg gcggctcggc gcaacagcag
     3241 gcgggagatg gccatggaca ctgaaccgct cgtctttgtc ggcatcgact gggcgagcac
     3301 cgaacaccaa gtttgcttga tgggagccgg agagccgatc cagcgagcgt tcgcccacga
     3361 cgccgccggg atcggcgcca tggtcgactg gttgtgcggc caggccaagc agtccggcca
     3421 agtcgccgtt gcaatcgaga cgccgcatgg gccggttgtc gaggcgctga tggaccgcgg
     3481 catcgccgtg ttcgccatca accccaagca gctcgaccgt ttccgcgacc gcttctcgcc
     3541 ggccggtgcc aaggacgacc gccgcgacgc tctggtgttg gcctcgtccc tgcgcaccga
     3601 ccgccactgt tttcggcgtg tggacgcact ggacccggcc gtcgtggaat tgcgcgagtg
     3661 gtcgcgcatg gccgaggagc tcaagggcga gcgtgtccgt ctggccaacc gcgtccgtca
     3721 gcagctttgg cgatactacc cgcaggccct cgatcttacc gacgacctcg gcgccgactg
     3781 ggtcctggcg ctgtgggcca aggcaccgac gccagctgac gcccgacgtt tgaccgagaa
     3841 gaaggtcgcc cgagtgctgg ctgcacaccg catccgccgc atcaccgcgg ccgatgctct
     3901 ggccatcctg caacggccag cgctgaccgt ggcgcccggc acggccgagg ccgcccgcgc
     3961 ccacatcacg gctacggccg agcgcctcta cctggtcaat cggcagatca aggacgtcac
     4021 ccgtcgtctg gacgcccttg tcgagcaact ggccggaccg gagcccgagc cggggcaagc
     4081 ggccgagcag cgcgacgcga cgatcctgcg ctccttgccg ggggtgggaa ggatcgtcct
     4141 cgccacgctg ctcgcggagg cacaccaagc catccaggcg cgagactatc atgccctgcg
     4201 gacgttggcc ggtgtcgcac cagtcaccaa gcgcagcggc aagtcccgcc gggtcgagat
     4261 gcggcaggcc tgttccaacc gcctgcgaac ggccgtctac cattgggcca gggtcgccac
     4321 ccagcatgac gtccgtagcc gcagccgata tgccgcccta cgcgcccgag gacactccca
     4381 tggccgtgcc ctgcgcaccg tcgcagaccg gttgctcggc atcgcctgca tcatgctcac
     4441 gaaccgcacc acctacgaac cgacgcacga ggcaactcgt gccccaaaaa ccacttgatc
     4501 actggtgggg agtcctcccc ctaactccct cccgcaaggg gagggggaag ctgccgaatc
     4561 tctcccgcac tcccgcgagt acgcgccgca tgatccccaa cgtcctcagc atcgccggtt
     4621 ccgatcccag tggcggggcc ggcatccagg cggacctgaa gagcttcgcg gccatgggcg
     4681 cctatggcat ggcggcgatc accacgctga ccgcgcagaa cacccagggc gtgcgcggct
     4741 tcgtcgcgat ggaaccggat ttcgtcaccg cgcagatcga cgcggtgttc gacgatatcc
     4801 gtgtcgatgc ggtgaagatc ggcatggttg ccaccgcgcc gatcgcccgc gccatcgccg
     4861 cggcgctgcg ccggcacaag ccggacaacg tcgtgctgga cccggtgatg gtcgccacca
     4921 gcggcgaccg cctgttgcgc gacgacgccg tcgccgcgat ccgcgacgaa ctggcgccac
     4981 tggccacggt catcaccccg aacatgccgg aagccgccgt gctgatcggt tccgggggcc
     5041 cggattcact ggctgccatg cgtgccatgc tgcccacgct gcaccgcctc ggttgcgaat
     5101 gggtgctgct gaaggccggc cacctggaca gcgacgatcg cacggacctg ctgttcgacg
     5161 gcactacagt caccgaactc cccggccgcc gcatcgatac ccgcaacacg cacggcaccg
     5221 gctgttcgct gtcatccgcc attgccgcgc tgctgccgcg ccatccggtg ccggaggccg
     5281 cgcggcgcgc caaggcgtgg ctcaccggtg cgctggccgg cagcgatcaa ctggatgtgg
     5341 gcagcggcca cgggccggtg caccacttcc atgcactctg gccgaaggaa cccgcagcat
     5401 gaccggattt tccgaggccg cctggcagcg cgttgccccg ctgcgtgcgg ccatccacgc
     5461 gctgccgttc aacaccgaac tcgccgccgg taccctcagc agcgcgcgct tccagggcta
     5521 catcatccag gacgcgctgt atctgacaca gtattcccgc gtgctggcgc tggccggcgc
     5581 acgcgccccg gacggtgccg cgctgcatgc cttcgcgtcc tcggcgctgg aggcggttgc
     5641 ggtcgagcaa gcgctgcacg agcgctacat ggcgcagttc ggcgtcgatc ccgcgcggct
     5701 tgccacggcg gagccgtcgc ccgactgcct gggctacacc agtttcctgc tggcctgcgc
     5761 ttatcacgag ccgtgggaaa tcctgctcgc ggccttgctg ccgtgcttct ggatctactg
     5821 ggatgtcggc accgcggttg cacgccgggc cgcggcggac aatccgtatc gcgcctggat
     5881 cgacacctat gcggacgagg cgttcggcga agcggtgcgc acggtgatcg ccataacgga
     5941 ccgcgcggcg gcggcaatga cggacgcggt gcgcgaccgc atgatgcgtg cgttcatccg
     6001 ctccagccag ttcgagtggc tgttctggga cggcgcctac cagcagcgcg gctggccggt
     6061 gggattgtga tattaagcga cggccggagt gatcgacgtg tcatggccgc gcttggcgcg
     6121 gccacccacg gctttttgct gctgcgccta caaaggcgtg ggtacccgtg ccaagcacgg
     6181 gcatgacaca aagatcggca tcatcgccgc ccgggacaac cggcgctacc cgcgcgcatc
     6241 gcacgccgcc accagtgcgt cgaaaatccg ccggtcgccc ggatcgatga agaactccgg
     6301 atgccactgt acgcccaggc agaaccggta gcgcgtatcc tcgatgccct cgatcaccgt
     6361 atccggcgcc accgcgttca ccacggcatg cggcccggga tcgcgcaccg cctgatggtg
     6421 cgcggaattc acccgcatct cggttgcacc gacgatccgg tgcagcagcg tgccgcgcgt
     6481 caccgcgaca gcgtgtcccg gctcgtgccg cggattgggt tgttcgtgct ccagtgcctc
     6541 cgggatggtg tccggaatgt gctggatcag cgtgccgccc agcgccacgg cgagcagctg
     6601 ctgcccgccg cagatgccca gcaccggcag ggtccgctcc agcgcgccac gcgtcagtgc
     6661 cagctcggct gcggtgcgtc cttccttcaa cgtcaccgtc gcatgccggt cgccgacgcc
     6721 atacagcgcc ggatcgacgt cgaaggcgcc gccggtcacc accagcgcgt cgatccggtc
     6781 gaggaacgcc tccgccagca ccggctcgtg cggcagggcc accggcaggc cgccggcggc
     6841 atcgatggca tcgagatagt tctggcgcag tgcgtaccag ggaaacttgg agtagccgcc
     6901 gggcttttcc g
//
LOCUS       DASSZH010000290         4026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_774020, whole genome shotgun sequence.
ACCESSION   DASSZH010000290 DASSZH010000000
VERSION     DASSZH010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774020"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..86
                     /locus_tag="VFN42_06230"
                     /pseudo
     CDS             1..86
                     /locus_tag="VFN42_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542453.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(172..1167)
                     /locus_tag="VFN42_06235"
     CDS             complement(172..1167)
                     /locus_tag="VFN42_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HET8996248.1"
                     /translation="MASANRRKDETDRAVRLRLKWVAAYQELRDAGAVCRRFGVSRPT
                     LRKWLRRYEADGEAGLMELSRRPHASPARKVGVAQEQVILDLRRARRLGVKRLRIELE
                     RLHGLRLAASTIHKALARHGLGALPRKRRSRHVPKRYSRPVPGDRVQMDTCKIRPGLW
                     QFAAVDDCSRYLVAGLAKRRSAAATLTFLDQVFEEMPFAIQRVQTDRGTEFFAKAVQR
                     RLMSETVKFRPTPPRSPHLNGKIERAQRTVLEEFWATVDPRTPDIADRLAEWVHHYNW
                     HRPHEALGGLSSIDRVCERIPKTPIWAEVEDSYDPAKERMQVREHAVDVALRALK"
     gene            1413..1799
                     /locus_tag="VFN42_06240"
     CDS             1413..1799
                     /locus_tag="VFN42_06240"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET8996249.1"
                     /translation="MEIITGVERRRRWRLEEKLRIVAEAEAPGACFAAVARRHEISRG
                     LLWNWRRQVRTGALGPAAAAFLPVRIVPGQTAPAVAAAPAVASRQQAAGGDTTIEIAL
                     PDGTTVRVGRDVGAVALRRVLGALRR"
     gene            1796..2143
                     /gene="tnpB"
                     /locus_tag="VFN42_06245"
     CDS             1796..2143
                     /gene="tnpB"
                     /locus_tag="VFN42_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183759.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HET8996250.1"
                     /translation="MIPVSSGVRVWLATGVTDMRRGMNGLALQVQQALGRDPHGGDLY
                     VFRGKRGDLIKVLWHDGLGMSLYAKRLERGRFIWPSPADGVVAITAAQLGYMLEGIDW
                     RHPQRTWRPEMAG"
     gene            2258..3781
                     /locus_tag="VFN42_06250"
     CDS             2258..3781
                     /locus_tag="VFN42_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HET8996251.1"
                     /translation="MAAEQLARREAEARACGAEAMVAHLKLLIAKLRHDRFGASSERG
                     RKLLDQMELELEELEAAASEDEATAAREPQNVSGVPRRRPVRGPLPAHLPRERVVLPS
                     PAACPCCGGKLAKLGETITETLEVIPRRWKVIQTVREKWSCRACETITQPPAPFHPIA
                     RGRAGPHLLAMVLTDKFADHLPLNRQSDAYAREGIELDVSTLADWVGACSATLAPLLG
                     LIRAHVLAGGRIHGDDTTVPVLAKGKTVTGRLWTYVRDDRPFGGSAAPAAMFAYSRDR
                     TAEHPRRHLEGWQGILQADAYAGFNGLYDAARQPGPIINAGCWAHARRNFFELAEVAK
                     APLAVEAVRRIDAIFAAERNSNGLPPEQRLAVRRHAIAPLLADLQTWMRAVRAKMSRH
                     AEVAKAIDYMLRRWDTFSRFIQDGRVCLSNNAAERALRGVALGRKAWLFAGSDRGGER
                     AAAIYSLIVTAKLNDVDPRAWLADVLGRIADHPASRLSELLPWNWREHIRDRDAAAA"
BASE COUNT          608 a         1382 c         1393 g          643 t
ORIGIN      
        1 aagccttcta caccatccgc tcggagcggc agctggtcga gcgcatcgac ttcgacctgc
       61 tgttccgctg gtttgtagaa gtttcgactt tatctgacgc cctggcgtga tccggcgtgg
      121 cgggtgtccg ccggatcgaa actgtcagcc gaagtgtgcc gttcgcagac atcatttcaa
      181 cgctcgtaac gcaacgtcta cggcatgctc gcggacttgc atgcgttcct tcgctgggtc
      241 gtagctgtcc tccacctcag cccagatcgg ggtcttgggg atgcgctcgc agacccggtc
      301 gatcgaagac aggccgccca atgcctcgtg cggtcggtgc cagttgtagt ggtgcaccca
      361 ctcggccagc cgatccgcga tgtccggcgt gcgcgggtcg acagtcgccc agaattcttc
      421 gagcacggtc ctctgtgcac gctcgatctt gccattcagg tgcggcgagc gcggtggcgt
      481 cggacggaac ttcaccgtct cggacatgag gcgccgctga accgccttgg cgaaaaactc
      541 cgtcccgcgg tcagtctgca cccgctggat cgcaaacggc atttcctcga atacctggtc
      601 aaggaacgtc agggtcgccg ccgcgctcct gcgcttcgcc aagccagcca cgagatagcg
      661 gctgcaatcg tcgacggcag cgaactgcca cagcccggga cggatcttgc acgtatccat
      721 ttgcacccgg tcgcccggca ccgggcggct gtagcgcttc ggaacgtgcc gtgaccgccg
      781 ctttcgtggc agcgcgccga ggccgtggcg ggccagagcc ttgtggatcg tcgaggccgc
      841 aaggcgcagg ccgtgcagac gctcgagctc gatgcgcagc cgtttgacac caagccgccg
      901 cgcgcggcgc aagtcgagga tcacctgctc ctgagccacc ccgaccttcc gagccgggga
      961 agcgtgtggc cgacggctca gctccatcag acccgcttca ccgtcggcct cgtatcggcg
     1021 cagccacttc cgcagcgttg gccgggagac gccgaaccgg cggcagaccg ctccggcatc
     1081 ccggagctcc tgatacgctg cgacccactt cagccgcagc cgaacggcgc gatccgtttc
     1141 atctttcctg cgatttgctg atgccatcgc tgctgtggtg gctgtgggat tgtgggcaac
     1201 gcgcagcgtt gtccatcaag tccacagcct accgaatcca ttgaaaggat gtctacgaac
     1261 cgtacagacc aggaatatcc cctccggaga ggctgacgaa tcattggcgg aggtgtatcc
     1321 gcccggcgac ggccgccttg cggcggtgaa tcccgggcct gaccttagcg gcgcttgtac
     1381 gaacgccgtt aaggtcggga gcgggttgcg cggtggagat catcaccggg gttgagcgtc
     1441 gtcgccgctg gcggctggag gagaagctgc ggatcgtggc cgaggcggag gcgcctgggg
     1501 cctgcttcgc cgcggttgcc cggcggcatg agatcagccg cggtttgctg tggaactggc
     1561 gtcggcaggt gcggaccggc gcgctcggcc cggcggcggc ggcgttcctg ccggttcgga
     1621 tcgtgccggg gcagaccgcc ccggcagtgg cggcggctcc ggcggtcgcg tcgcgccagc
     1681 aggcggcggg cggggacacg acgatcgaga tcgcgctgcc ggacggcacc acggtgcggg
     1741 tcgggcggga tgtcggcgca gtggcgctgc gccgcgtgct gggggcgttg cgccggtgat
     1801 cccggtttcc tccggcgtgc gggtgtggct ggcgaccggc gtcaccgaca tgcggcgcgg
     1861 catgaacggg ctggcgctgc aggtgcagca ggcgctcggc cgcgatccgc atggcggcga
     1921 cctttacgtc ttccgcggca agcgcggcga cctgatcaag gtgctctggc acgacgggct
     1981 cggcatgtcg ctttacgcca aaagattgga acgtgggcgt ttcatctggc cgtcgcccgc
     2041 ggacggcgtg gtggcgatca cggcggcgca gctcggctac atgctggaag gcattgactg
     2101 gcggcatccg cagcgaacct ggcgccccga gatggccgga taggccgcat cggcggcata
     2161 tcgggggtac acaaccgtcg ccgcctgtga ttcactcatc ggcgtgacgg acaacgccat
     2221 cccgattccg gacgatgtca ccgcgctgcg ggccgcgctg gccgccgagc aactggcgcg
     2281 gcgcgaggcc gaggcccgcg cctgcggcgc cgaggcgatg gtggcgcacc tgaagctgct
     2341 gatcgccaag ctgcggcatg accggttcgg cgcctcctcc gagcgcggcc gcaagctgct
     2401 cgaccagatg gaactggaac tggaagagct ggaggcggcc gccagcgagg acgaggcgac
     2461 ggcggcgcgc gagccgcaga acgtatccgg tgtcccccgc cgtcgcccgg tgcgcggccc
     2521 gctgcccgcc cacctgccgc gcgagcgcgt ggtgctgccc agccccgccg cctgtccgtg
     2581 ctgcggcggc aagctggcga agctgggcga gacgatcacc gagacgctgg aagtgattcc
     2641 gcgccggtgg aaggtgatcc agacggtgcg cgagaagtgg agctgccggg cctgcgagac
     2701 gatcacgcag ccgcctgcgc cattccaccc gatcgcgcgc ggccgcgccg gaccgcacct
     2761 gctggccatg gtgctgaccg acaagttcgc cgatcacctg ccgctgaacc gccagagcga
     2821 cgcctatgcg cgcgagggca tcgagctgga cgtgtccacc ctggccgact gggtgggcgc
     2881 gtgctcggcc acgctggcgc cgctgctcgg gctgatccgc gcgcatgtgc tggcgggcgg
     2941 gcggatccat ggcgacgaca ccacggtgcc ggtgctggcc aagggcaaga ccgtcaccgg
     3001 ccgattatgg acctatgtgc gcgacgatcg tccgttcggc gggagcgccg cacccgcggc
     3061 gatgttcgcc tactcccgcg accgcaccgc cgaacatcca cggcggcacc tggagggctg
     3121 gcagggcatt ctccaggccg atgcctatgc cgggttcaac ggcctctacg atgccgcccg
     3181 ccagcccggg ccgatcatca acgcgggctg ctgggcccat gcgcgccgca acttcttcga
     3241 actcgccgag gtcgccaagg cgccactcgc cgtcgaggcg gtgcggcgga tcgacgcgat
     3301 cttcgccgcc gagcgcaaca gcaacggtct gccgccggag cagcgtctcg ccgtgcgccg
     3361 ccatgccatt gccccgctgc tggccgatct gcagacctgg atgcgcgcgg tgcgcgcgaa
     3421 gatgtcgcgc catgccgagg tcgccaaggc catcgactac atgctcaggc gctgggacac
     3481 gttcagccgc ttcatccagg acggccgggt ctgcctgagc aacaacgcgg ccgagcgcgc
     3541 gctgcgcggt gtggctcttg gacgaaaggc gtggttgttc gccgggtccg atcgcggcgg
     3601 cgagcgcgcc gcggccatct actcgctgat cgtcacggca aagctcaacg atgtcgatcc
     3661 gcgtgcctgg ctcgccgacg tactcggacg catcgccgat cacccagcat cgcgcctcag
     3721 cgaactcctg ccctggaact ggcgcgagca cattcgggac cgcgacgccg cggcagcctg
     3781 atagctacgc cgcggcactc gccggatgga tacgggcctt caccgcgatc cgctgggccg
     3841 acaacggcgg ggcggctctc tgcccgcact gcgaatgcac caaggtctat gaatgccgcc
     3901 ggccgaacgg tgcgctccgc taccgctgca aggcgtgccg caaggatttc tccgtcacgt
     3961 ccggcacgct gttctcgcac cacaagctac cgctgcggac ctacctcgcg gccatcgcca
     4021 tcttct
//
LOCUS       DASSZH010000291         2703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1336314, whole genome shotgun sequence.
ACCESSION   DASSZH010000291 DASSZH010000000
VERSION     DASSZH010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336314"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="VFN42_06255"
     CDS             <1..1083
                     /locus_tag="VFN42_06255"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET8996252.1"
                     /translation="AAPQEALDRLLTLLRHAERPIIIAGSGALWSLAADHLHCFVNAT
                     GIPFYTTPPARGLVPEDDACCFPGARSKAFAAADLVLIIGSRLNYVLGFGQAPRFAAN
                     AKLVRIDIDPEEIGRGNQHLGIVGDARTVLRQLCDRLPMYFSTANYAQWRATLAAADA
                     AHDEAQRALVAQQSEPIHPLQLCLAVREMLPRDAIVVADGQEILHYSRQSMRSFAPGH
                     RLTPGPFGTMGVGVPFALGAKAASPNASVVVMQGDGAFGLNAMELDTAVRHKLPVLVV
                     ISLNGGWTADAGDGRIGRALAGIRYDRLAESLGCHGEFVTRAEDIRPALERALLAMQQ
                     GQPAVVNVVTDACARAHTTGYARYET"
     gene            1132..1665
                     /locus_tag="VFN42_06260"
     CDS             1132..1665
                     /locus_tag="VFN42_06260"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HET8996253.1"
                     /translation="MPILERARSALLLVDFQARLMPAIDDATQVVANARRLLDAAQLF
                     DIPVMLTEQNADGLGPTVPELRSAAARTAAKMTFDATRMPGFLAALPDRPDLVIAGCE
                     THVCVLQTVLGLLRAGRRIVVVRDAVGSRRAESKEAAINRMERNGAEIVTTEMVLFEW
                     LETATDKRLRAVLDLVR"
     gene            complement(1691..2257)
                     /locus_tag="VFN42_06265"
     CDS             complement(1691..2257)
                     /locus_tag="VFN42_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HET8996254.1"
                     /translation="MCAATAGREQYFTIGHSTRSIAELVELLREAGADCVVDVRSIPR
                     SRTNPQFNADTLPAALAAEKLGYCRIAELGGLRKRRKGEEESPNAFWENLSFRNYADY
                     TATAEFRSGLAALRNLAARHRPAIMCAEAVWWRCHRRIIADYLLAAGAEVRHILAHGK
                     IEPAHMTESAVVQPDGSLIYPPQDLRLH"
     gene            complement(2318..>2703)
                     /locus_tag="VFN42_06270"
     CDS             complement(2318..>2703)
                     /locus_tag="VFN42_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996255.1"
                     /translation="LAEVHRHNLAVLMFGDPARVDRLATWLQIENVRTARGRAGGFPA
                     SDALRRVLPQVKARLYGIWGERDAFAAPYLVDRETLLRSLHPELRFHVIAGAGHWTPY
                     EAAASVNDTISDMLGSPNAGPRRDA"
BASE COUNT          450 a          888 c          899 g          466 t
ORIGIN      
        1 gcggcaccgc aggaggctct cgatcggctg ctgacgctgc tccgccacgc ggagcggccg
       61 attatcatag cgggcagcgg cgcgctctgg tcgctcgctg ccgatcactt gcactgcttt
      121 gtcaatgcca caggcattcc cttctacacg acaccaccgg cgcgcggcct tgtgccagag
      181 gacgacgcat gttgcttccc cggcgcacgc agcaaggcct ttgccgcagc ggatcttgtg
      241 ctgatcatcg gctcgcgctt gaattatgtg ctcggcttcg gccaggctcc gcgcttcgcg
      301 gccaacgcca aactggtccg catcgacatc gaccctgagg agatcggccg cggaaaccaa
      361 cacctcggca ttgtcggcga cgccaggacg gtgctgcggc agctatgcga ccggttgccg
      421 atgtacttca gcaccgcgaa ctacgcccag tggcgcgcca cactcgccgc ggcggatgcc
      481 gcacacgacg aggcgcagcg cgccctggtg gcgcaacagt cggaacctat ccatccactg
      541 cagttgtgcc tggcggtccg cgagatgctt ccacgcgacg ccatcgttgt ggcggacgga
      601 caggagatcc tgcactactc acgacaatcc atgcggagtt tcgctcccgg tcaccggctg
      661 acgcccgggc cgttcggcac catgggcgtc ggtgtgccgt ttgccctggg ggcgaaggcc
      721 gcgagcccca acgcttcagt ggtggtcatg cagggcgacg gtgccttcgg actgaacgcg
      781 atggaactgg ataccgcggt gcgtcacaag ctgccggtcc tggtggtgat cagcctgaac
      841 ggcggctgga ccgcggatgc gggcgacggc cggatcggtc gggcgcttgc cggcatccgt
      901 tatgaccggc ttgccgaatc gctcggttgc cacggcgagt ttgtcacgcg cgccgaggac
      961 atccgtccgg cgctcgaacg ggctctgctg gccatgcagc aaggccagcc ggctgtggtt
     1021 aatgttgtca cggatgcatg tgcgcgggcg cacaccacag gctacgcgcg ttatgagacc
     1081 tgagccgtgg acgcggccgg cgtcccaatg aaacggagga gatagcgaca catgccgata
     1141 ctcgagcgcg ccaggtcggc gcttctgctg gtggacttcc aggcgcgact gatgcccgcg
     1201 atcgatgacg ccacgcaggt ggttgccaat gcccggcggc tgctcgatgc ggcgcaattg
     1261 tttgacatcc cggtgatgct caccgagcag aacgccgatg ggctggggcc gacagtgccg
     1321 gaactgcgat ccgctgctgc ccgcaccgcc gccaagatga cgttcgatgc cacccgcatg
     1381 cccggctttc tcgcagcgct gcccgatcgc cccgacctgg tcatcgcagg atgcgagacg
     1441 catgtctgcg tgctgcagac ggtgcttggt ctgctgcggg ccgggaggcg catcgttgtc
     1501 gtgcgcgacg ctgtcggttc acgccgggcg gaaagcaagg aagccgccat caaccgcatg
     1561 gagcgcaacg gggcggaaat cgtcaccacc gaaatggtgc tgttcgaatg gctggaaacc
     1621 gcgaccgaca agcggttgcg tgcagtgctc gacctggtca ggtaggcgca agcctggcag
     1681 gcgcggctgc tcagtgcaat cggagatcct gtggcggata gatcaggctg ccatccggct
     1741 gcaccaccgc ggactcggtc atgtgcgccg gttcgatctt gccgtgagca aggatatggc
     1801 gaacctcggc gcccgcggcc agcaggtagt cggcgataat gcgccgatgg caccgccacc
     1861 ataccgcctc ggcgcacatg atcgcgggac gatgccgcgc cgccagattg cgcaatgcgg
     1921 cgagcccgct gcggaattcg gccgtggcgg tatagtcggc atagttgcgg aagctgagat
     1981 tctcccaaaa agcgttcggc gactcttcct cgcccttccg ccgtttgcgc aacccgccaa
     2041 gttcggcgat ccggcaatac cccagcttct ccgccgccaa tgccgcaggc agggtatcgg
     2101 cattgaactg cgggttggtt ctcgaccgcg gtatgctgcg gacatccacg acgcaatccg
     2161 cgcccgcttc gcgcagcagt tcaaccagct ccgcgatcga ccgggtcgag tgcccgatgg
     2221 tgaagtattg ctctctgcct gcagttgctg cgcacattgc tgcacggcct tccggttggt
     2281 gtggcccgcc gctgcctggg gcgcgagcca ttcgcggcta cgcgtcgcgg cgtgggccag
     2341 cgttcggtga gcccagcata tccgatatgg tgtcgttcac gctggcagca gcctcatacg
     2401 gtgtccaatg cccggcaccg gcgataacgt ggaaccgcag ttcaggatgc aggctgcgca
     2461 gcaatgtctc gcggtccacc aaatacggag cggcaaaggc gtcgcgttcg ccccagatcc
     2521 cgtacagccg cgctttgacc tggggcagca cccggcgcag cgcatcggag gcgggaaagc
     2581 cgccagcacg gccgcgcgcg gtgcggacat tctcgatttg caaccaggtg gcgagtcggt
     2641 cgacgcgggc cgggtcgccg aacatcagca ccgccaggtt gtgccggtgc acctcggcga
     2701 gct
//
LOCUS       DASSZH010000292         2130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_944620, whole genome shotgun sequence.
ACCESSION   DASSZH010000292 DASSZH010000000
VERSION     DASSZH010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944620"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="VFN42_06275"
     CDS             <1..1027
                     /locus_tag="VFN42_06275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET8996256.1"
                     /translation="TMDMSGPSPKLDIERVLEAERLGFSQAWTGEAYGTDAVTPAAWM
                     LARTTRINVGTGIMQIPARTPACAAMTAMTLQLLSSGRFLCGLGVSGPQVVEGWHGVR
                     FGKPMTRTKEYIAIIRKILERKAPLEFEGAEYVIPYAGPGATGLGKPLRSITHGDPSL
                     KFYMASITPAGLRTAGECADGVLPIFYSPEQPNVVSDPILEGKKKAGRPVTISDFDVA
                     PYVRVKLGADVAACRDAIRPELALYIGGMGARAKNYYNDVAIKLGYEAEAKTIQDLYL
                     DGKKTDAAAAVPDRLVDEISLVGPAERIKDRLQAWKEAARSGHLGTMVLKGASIEAMR
                     VVAEAVL"
     gene            complement(1062..>2130)
                     /locus_tag="VFN42_06280"
     CDS             complement(1062..>2130)
                     /locus_tag="VFN42_06280"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET8996257.1"
                     /translation="WAGDADAQLELGSVLSHRPEEAEQARKWLLAAAGRHEAVAELLA
                     GLGYDRAQNGFPRDFAQAAHWLQAGLADIARTPEAGAYSEKFSLRLVDALWGDSALEV
                     GLMAYNGVVLPRDMAHAAALFRLAAAHHSAKAAAILGDMYLDGKLGAPDPVQAVPWYR
                     QAAEAGQPNAMNQLGMLYTLGRGVAKNPAKAAYWYRFGAQHGNAASEQQLAALFWFGA
                     DGMPLDRARAVKWLRRAAEQGQVWSQSTLAVAYRTGMGVPLDYAQSFAWAQRAAVQGD
                     ALSENTVGYALLIGRSVPEDAARACVWLQLAVERSKPGELKDRAKVNLARAREQLTAE
                     QAGSCANQAAEWQARFGREGH"
BASE COUNT          346 a          758 c          706 g          320 t
ORIGIN      
        1 caccatggac atgtccggtc cgtcgcccaa actcgacatc gagcgggtgc tcgaagcgga
       61 gcgtctcggc ttttcccagg cctggaccgg cgaggcgtac ggcaccgatg cggtgacgcc
      121 cgccgcgtgg atgctggcac gcaccacgcg catcaatgtc ggcaccggga tcatgcagat
      181 tcccgcgcgc accccggcct gcgcggcgat gaccgcgatg acgctgcagc tgctgtccag
      241 cgggcgcttc ctgtgcgggc tgggcgtgtc aggaccgcag gtggtggaag gctggcacgg
      301 cgtgcgcttc ggcaagccga tgacccgcac caaggaatac atcgcgatca tccgcaagat
      361 cctggagcgc aaggcaccac tggagttcga gggcgccgaa tacgtcatcc cctacgccgg
      421 ccccggcgca accggcctgg gcaagccgct gcgcagcatt acccatggcg acccgtcgct
      481 gaaattctac atggcgtcga tcaccccggc cggcctgcgc accgccgggg aatgcgcgga
      541 tggcgtgctg ccgatcttct actcgccgga gcagccgaac gtggtgtccg atccaatcct
      601 ggagggaaag aagaaggcgg gtcgtcccgt caccatctcc gatttcgacg tcgcacccta
      661 tgtgcgcgtc aagcttggcg ccgacgtggc ggcgtgccgc gatgccatcc gcccggagct
      721 ggcgctgtat atcggcggca tgggggcgcg ggcgaagaac tactacaacg acgtggctat
      781 caagctgggc tacgaggcag aggcgaagac gatccaggac ctgtatctcg acggcaagaa
      841 gaccgatgca gccgcggcgg tgccggaccg gctggtcgac gagatctccc tggtgggtcc
      901 ggcagagcgg atcaaggacc gcctgcaggc ctggaaagaa gcggcgcggt cgggccacct
      961 gggcaccatg gtgctgaagg gcgccagcat cgaggcaatg cgggtggtgg cggaagcggt
     1021 gctgtagctc cgccctgcca ccggccccga ggtgagcgga gtcaatgccc ctcccggccg
     1081 aaccgcgcct gccattcagc cgcctgattc gcacagcttc cagcctgttc ggcggtgagt
     1141 tgttcgcgcg cgcgggccaa gttcaccttc gcacggtcct tcaactcgcc cggcttggac
     1201 cgctccaccg cgagctgcag ccagacgcag gcgcgcgcgg cgtcctccgg caccgatcgg
     1261 ccgatcagca gcgcataacc gaccgtgttt tccgacagcg catcgccctg caccgccgcg
     1321 cgctgcgccc aggcgaagga ctgggcgtaa tccagcggga cgcccatgcc ggtgcggtag
     1381 gccaccgcga gcgtgctttg cgaccagacc tggccctgct ctgccgcgcg gcgcagccat
     1441 ttcaccgccc gcgcccggtc cagcggcatg ccatcggcgc cgaaccagaa cagcgcggcc
     1501 aattgctgct cggaggcagc attgccgtgc tgcgccccga atcggtacca gtacgccgcc
     1561 ttggccggat tcttcgccac gccgcggccg agcgtgtaca gcatgccgag ctggttcatc
     1621 gcgttcggct gcccggcttc cgctgcctga cgataccagg gcacggcctg caccgggtcg
     1681 ggcgcgccca atttcccgtc gagatacatg tcgcccagga tcgccgcggc cttggcggaa
     1741 tggtgtgccg ctgccagccg gaacagcgcc gcggcatgcg ccatgtcgcg cggcaacacg
     1801 acgccattat acgccatcaa tccaacctcc agcgcgctgt cgccccacag cgcatccacc
     1861 aagcgcagcg aaaacttttc gctgtaggct ccggcttcgg gtgtacgcgc gatatcggcc
     1921 agccccgcct gcagccaatg cgccgcctgg gcgaagtcgc gcgggaagcc gttctgggcg
     1981 cggtcgtagc ccaggccggc gagcagctcg gccaccgcct catgccggcc ggccgccgcg
     2041 agcagccact tgcgcgcctg ctctgcctct tcgggccggt gcgacagcac tgagcccagt
     2101 tccagctgcg cgtcggcatc gccggcccag
//
LOCUS       DASSZH010000293         4996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_126409, whole genome shotgun sequence.
ACCESSION   DASSZH010000293 DASSZH010000000
VERSION     DASSZH010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126409"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(456..1508)
                     /locus_tag="VFN42_06285"
     CDS             complement(456..1508)
                     /locus_tag="VFN42_06285"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996258.1"
                     /translation="MAAQSYVYAGVAGYFGRQGEPGQVGAFRRAADGGDWEHVVTDHE
                     TFTVAVHRADANLVFAGTNDGVYRSTDRGRTFRRTNFPDGNMQIWSIMADPADRTVIY
                     AGGSPVAVYRSEDSGESWKRMPDPGMPNRAVMPFACRVMRMAPHPRHAGEIYAALEVN
                     GVMHSKDGGESWTDCSEGLVRLAQQPHLKSRIVSDTENEGMLDGHAICTTTADPDAVI
                     VAVRMGLFRSTDEGADWQDMEVGRFSPTTYGRDVKVSPQDPNTLYAALSVAASSKDGG
                     VYRSTDVGKTWQRFDKVQVHGTIMSVALHQNDPQQLYIGARYDGEIFGTRDGGATWQS
                     MPLPQGVKDIYSVACG"
     gene            1627..2676
                     /locus_tag="VFN42_06290"
     CDS             1627..2676
                     /locus_tag="VFN42_06290"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HET8996259.1"
                     /translation="MPTVQAERLMEIARGLLQAAGASAEEAATIARYNIGANLVGHDS
                     HGIILIPTYIDRIKNNHIVPGAPWVITQETATTTVVDGNWGFGYVVTDRAMRHTIDKA
                     KKQSVAATTVYRQSHIGRLASYPLMAAQEGMIAMITADSGRSAKAVAPFGGAKARLGT
                     NPICFAIPSNLEGPLFFDMATSAAAAGKINVATARGEQVPAGWLIDAEGKPSTDPRVL
                     KQGGALLPVGGTEGYKGYGLATIVEILSGLLTGLGFGVEPSGRHNDGCFIAVFNVSAF
                     RDLTTFKQEVTEFAQYLKATPPAQGFTEVLYPGEIEFRREQDRRKNGIPVEDATWNKL
                     ASMAKEYGIAGQLGF"
     gene            2719..3135
                     /locus_tag="VFN42_06295"
     CDS             2719..3135
                     /locus_tag="VFN42_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HET8996260.1"
                     /translation="MRQCVHHLAVPTRGQGLVEVTEPIRRWVNQQSVGTGLLTVWCRH
                     TSASLLVQENADPDVRADLLAFFRRVVPENAGYRHQEEGPDDMPAHIKAALTQTQLSI
                     PVIEGGLALGTWQGIYLFEHRAAPHRRELVLHLLGE"
     gene            complement(3325..4011)
                     /locus_tag="VFN42_06300"
     CDS             complement(3325..4011)
                     /locus_tag="VFN42_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007425025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET8996261.1"
                     /translation="MSIILYELAGADPDLRFSPYCWRTRMALAHKGLPVETIPWRFTD
                     KDAIAFSGQGRVPVIRDGDTVVSDSWAIAEYLEERYPTPTLFGGAMGRAHARFINAWA
                     DAVLIGAIARLIVRDILDVADPKDRDYFRSSREARFGMTLEATQADREQRVGAFRDLL
                     LPVRLVLRGQQWLGGAAPSYADFIVFGTLQWPWCCSRFPLLAEDDPVAAWRERMLDLF
                     GGLGRGAKRA"
     gene            4108..4467
                     /locus_tag="VFN42_06305"
     CDS             4108..4467
                     /locus_tag="VFN42_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996262.1"
                     /translation="MAAPPLLQLQEQFDEAGYLRLHPDVAQAIASGIGITAWQHYLGY
                     GKRERRRPNDVDPDFYGKAYPLAAAEIAAGGAVDAAAHYLRFGQARGYRPNAAAPRPA
                     PPTVFPGSGPTCRMPRT"
     gene            4530..>4996
                     /locus_tag="VFN42_06310"
     CDS             4530..>4996
                     /locus_tag="VFN42_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996263.1"
                     /translation="MQDGYVVFDDPYDPIALGAGALDLERGFAGARPELLFTCGAVAL
                     GPIRWQPEINPYPAAALDIHVMSRAVRNLLFAGQLAEFLGLLFDAPALLTHSRGMLRD
                     AVQPVQRDAAGVGYTLARQWVAAVVLLEDIDPAAMYVFPASHRLPVPPGQAVEP"
BASE COUNT          810 a         1755 c         1625 g          806 t
ORIGIN      
        1 ggaattcacg ccgtccgcgc cgatcgcgcg cgcagccgcc gccaccggtc cgccgtgatg
       61 ctgcacgcaa gcctacagca cggaacgcac gggaccggcc cacggccagc cccgccgttc
      121 cgattcgtgc cctgcgtggc ggcggtgtcc aagcccgctg ggctgaccgt ggccgtactc
      181 cgtcatggcg ggcgaaggcc cgcagtacag cgtcatggcg ggcgaaggcc cgccatccac
      241 gatttgttgc aggagactcc gagccgcaat ccgcctcgga gtctgcccca gccagtcatg
      301 gatggcgggc cttctcccgc caagacgatg gacgacgggc caacagcgca tgtttccgcc
      361 tataacgcag gcccgccacg cacgattttg ccgcgtgcag ctgaggaaaa gcgcgcgcgc
      421 tcgtggacgg ccggcttgcg ccggtcctga cgaggctacc cgcaggcgac cgaatagatg
      481 tccttcacac cctgcggcaa cggcatgctc tgccaggtcg cgccgccatc ccgcgtgccg
      541 aatatctcgc catcgtagcg cgcgccgata tacagctgct gcggatcgtt ctggtgcagc
      601 gccaccgaca tgatcgtgcc gtgtacctgc accttgtcga agcgctgcca ggtcttgccg
      661 acatccgtgc tgcggtacac cccgccatcc ttgctggaag cggcgacgct cagcgcggca
      721 tacagcgtgt tcgggtcctg cggcgaaacc ttcacatcgc gcccataggt ggtgggcgag
      781 aagcggccga cctccatatc ctgccagtcg gcgccttcat ccgtgctgcg gaacaggccc
      841 atgcgcaccg ccacgatcac cgcgtccgga tcggcggtgg tggtgcagat cgcgtgcccg
      901 tccagcatgc cctcattctc ggtgtcgctg acgatgcggc ttttcagatg tggctgctgc
      961 gccaggcgga ccaggccctc gctgcaatcc gtccagcttt caccgccatc cttgctgtgc
     1021 atcacgccgt tcacttccag tgcggcgtag atctctcccg catggcgcgg atgcggcgcc
     1081 atgcgcatca ccctgcaggc gaacggcatt acggcgcggt tcggcatgcc gggatcgggc
     1141 atgcgcttcc agctctcgcc ggaatcctcg ctgcgataca ccgccaccgg cgagccgccg
     1201 gcatagatca ccgtgcggtc tgccggatcg gccatgatcg accagatctg catgttgccg
     1261 tcagggaagt tcgtgcgccg gaaggtcctg ccgcgatcgg tgctgcgata cacgccgtcg
     1321 ttggtgcccg cgaacaccag gttcgcatcg gcccgatgca ccgcgacggt gaaggtctcg
     1381 tgatcggtga ccacgtgctc ccagtcgcca ccatcagccg cccggcggaa cgcgccgacc
     1441 tgtcccggct cgccctggcg gccgaaatac cccgcgacac cggcgtagac atatgattgc
     1501 gctgccatgg cacaccctcc ccttcaaggt tggcgacagg ctacgctttc cctggccggc
     1561 ggcaagcggg gttgactccg gtctgcccgc ccgccttgct gatcgcaatc gagaggggag
     1621 aacgccatgc cgaccgttca agctgaacgc ctgatggaga tcgcccgcgg actgctgcag
     1681 gccgccggtg cgtccgccga ggaagccgcc accatcgcgc gctacaatat cggcgccaac
     1741 ctggtcgggc atgattcgca cggcatcatc ctgatcccca cctatatcga ccgcatcaag
     1801 aacaatcaca tcgtgcccgg tgcgccctgg gtgatcacgc aggaaaccgc gaccacgacc
     1861 gtggtcgatg gcaactgggg tttcggctac gtcgtcaccg accgcgcaat gcgccacacg
     1921 atcgacaagg cgaagaagca gagcgtcgcc gccaccacgg tgtatcgcca gagtcatatc
     1981 ggccggctcg ccagctatcc gctgatggcg gcgcaggaag gcatgatcgc gatgatcacc
     2041 gcggattccg gccgcagcgc caaggccgtt gcgccgttcg gcggtgccaa ggcgcggctg
     2101 ggcaccaatc cgatctgctt cgccatcccg tccaacctgg aaggcccgct gttcttcgac
     2161 atggcaacct ccgccgcggc ggcgggcaag atcaacgtgg ccaccgcgcg cggcgagcag
     2221 gttcccgccg gctggctgat cgatgcggag ggcaagccct ccaccgatcc gcgcgtgctg
     2281 aagcagggcg gtgcgctgct gccggtgggc ggcaccgaag gctacaaggg ctacggcctg
     2341 gccacgatcg tcgagatcct gtccggcctg ctcaccgggc ttggcttcgg cgtcgaaccg
     2401 agcggccggc acaatgacgg ctgcttcatc gccgtgttca atgtttcggc attccgcgac
     2461 ctgaccacgt tcaagcagga agtcaccgag ttcgcgcagt atctgaaagc gacgccgccg
     2521 gcgcagggct tcaccgaagt gctgtatccg ggcgagatcg aattccgccg cgaacaggac
     2581 cgccgcaaga acggcatccc ggtggaagac gccacctgga acaagctggc gtccatggcg
     2641 aaggaatacg gcatcgccgg ccagcttggc ttctgaccgc gtcttgcgtt cggccgcgcc
     2701 aggtcacagc tctgcgtcat gcgacaatgc gtgcatcacc tggcggtgcc gacccgcggc
     2761 cagggcctgg tggaggtgac ggaaccgatc cgccgctggg tgaaccagca gtcggtcggc
     2821 accggactgc tgaccgtgtg gtgccggcac acttcggcgt cactgctggt gcaggagaat
     2881 gccgatccgg atgtgcgcgc cgatctgctg gcgttcttcc gccgcgtcgt gccggagaac
     2941 gccggctatc gccatcagga ggaagggccg gacgacatgc cggcgcatat caaggcggca
     3001 ctcacccaga cgcagctcag cattccggtg attgagggcg gcttggcctt gggcacctgg
     3061 cagggcatct acctgttcga gcaccgcgcg gcgccgcacc ggcgggagct ggtgctgcat
     3121 ctgctgggag agtgaacgcc ccgcgtcatg ttctgcaaag gccgacccgc gcgctccatc
     3181 atggccggcg aaggccggcc atccacgtct tccccggcac acaacagcaa ggcgtggatg
     3241 gtccgccttc gcggaccatg acggggggaa gcgccgtccg ccttcgcgga ccatgacaaa
     3301 gggacggtgc gctattcccg aaacttacgc ccgcttcgcg ccgcgtccca gtccgccgaa
     3361 caggtccagc atgcgttcgc gccacgccgc caccggatcg tcctccgcca gcagcggaaa
     3421 gcggctgcag caccacggcc actgcaacgt accgaacacg atgaaatccg catagctcgg
     3481 cgccgcaccg cccagccatt gctggccgcg caacaccagc cgcaccggca ggagcaggtc
     3541 gcggaacgca ccgacgcgct gctcccgatc ggcctgcgtg gcttccagcg tcatgccgaa
     3601 ccgcgcctcc cggctcgacc ggaagtaatc gcggtctttc ggatcggcca cgtccaggat
     3661 gtcgcgcacg atcaggcgag cgattgcgcc gatcagcaca gcgtcggccc aggcgttgat
     3721 gaagcgtgca tgcgcacgtc ccatcgctcc gccgaacagt gtcggtgtcg ggtagcgttc
     3781 ttccaggtat tcggcgatgg cccagctgtc ggacaccacc gtgtcgccgt cgcggattac
     3841 cggcacgcgg ccctggccgg aaaacgcgat cgcgtccttg tcggtgaagc gccagggtat
     3901 ggtttccacc ggcaatccct tgtgcgccag cgccatgcgc gtacgccagc aataggggct
     3961 gaaccgcaaa tcgggatcgg cgccggcgag ctcgtacagg atgatcgaca tgccggcact
     4021 ctgccgcggc gtcgcaggcc ggtccagcgt tgtccccgcc cgccgcgcca tgctagcggc
     4081 cagcccgcgc agcaggagga tcgtgacttg gctgcaccgc cgctcctgca gttgcaggaa
     4141 cagttcgacg aagcgggcta tctgcgtctg catcccgatg tcgcgcaggc gatcgcgtcc
     4201 ggcatcggga tcaccgcctg gcagcactac ctcggctacg gcaagcgcga gcgccggcgg
     4261 ccgaacgacg tcgatccgga tttctacggc aaagcctatc cgctggccgc agccgagatt
     4321 gccgctgggg gggcggtcga tgcggcggcg cattacctgc ggttcggcca agcacgcggt
     4381 tatcgtccga acgcggcggc accgcggccg gcgccgccga cggtcttccc gggctctgga
     4441 ccgacctgcc gcatgccgag gacctgatcg aaggccggtc gcggctcggc cggctgacgg
     4501 cacgccaggc cagcctgctg cgccgctggg tgcaggacgg ctacgtggtg ttcgacgacc
     4561 cgtacgatcc gatcgcgctc ggcgccggcg cattggacct ggaacgcggt ttcgccggcg
     4621 cccgcccgga gctgctgttc acttgtggtg cggtggcgct gggtccgatc cgctggcagc
     4681 cggagatcaa tccctatccc gcggcggcct tggacatcca cgtcatgtct cgcgccgtgc
     4741 gcaacctgct gttcgccggg cagcttgccg agttcctcgg cctgttgttc gacgcgcccg
     4801 ccctgctgac gcacagccgc ggcatgctgc gggacgccgt gcagccggtt cagcgcgatg
     4861 ccgcgggcgt gggctacacg ctggcccggc aatgggtggc ggcggtggtc ctgctggagg
     4921 atatcgatcc cgcggcgatg tacgtgttcc cagccagcca ccgcttgccg gttccccccg
     4981 ggcaggcagt ggaacc
//
LOCUS       DASSZH010000294         6943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_620824, whole genome shotgun sequence.
ACCESSION   DASSZH010000294 DASSZH010000000
VERSION     DASSZH010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6943
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620824"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VFN42_06315"
     CDS             <1..319
                     /locus_tag="VFN42_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8996264.1"
                     /translation="NFGMQFNGSLFDVLSWAIIKGSPNRQNAEQLLYFAGTPGIEAQL
                     FRATGEAGLAKGANDGLSANLLPMLPTMQNNLGAALRIDTAFWHQNLAKLQKRFDSWL
                     NAH"
     gene            complement(522..713)
                     /locus_tag="VFN42_06320"
     CDS             complement(522..713)
                     /locus_tag="VFN42_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996265.1"
                     /translation="MIVVSDITPGTKLRIAGGLVVEVLENMGDGEWLRVRLLQVPEGA
                     SAAVGSEELCHASDVIELA"
     gene            complement(716..1282)
                     /locus_tag="VFN42_06325"
     CDS             complement(716..1282)
                     /locus_tag="VFN42_06325"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: protein motif:HMM:NF007479.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HET8996266.1"
                     /translation="MTEVIERRPVRRGLAWFELKQEIEDFLYGEADLLDQRRFTEWLD
                     LLADDLVYFMPIRRNVKFGQHEERENTRQGEGISWFDEDKWTLTKRVEQILTGVHYAE
                     EPLSRVTHMVSNVRLLDVQPSAEAPQQVTVGCRFLVHQNRVEYETYSFIGRRTDVLRR
                     AGESWQIARRELILEQNVLLAKNLSILF"
     gene            complement(1288..2640)
                     /locus_tag="VFN42_06330"
     CDS             complement(1288..2640)
                     /locus_tag="VFN42_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HET8996267.1"
                     /translation="MATQLPLDQAIDLEHGMISREIFVSPDYHRAELEKLFTRAWLFI
                     GHESQIAKPGDFFVSRMGEESVILCRDAQGAVHVFLNSCRHRGMKVCRYEQGNTSLFV
                     CPYHSWSYTTDGKLQGVPLYRVLYENTLKREEWSLIEVPKLALYKGTVWASWDPQAPD
                     FMEYLGGAVDHLDQVLDCRDGRPGGSEVIGVHKWIFPANWKFAAENFLGDTYHNPSHR
                     SVDMIGINPSGQRGIKGRRDNEYNQGQHIWVGFPQGHGMHSVLMPEDMQYEPTYQHYP
                     IVDEYFRHCFHERQRRLGDKARLLPFVGNLFPNASYHGRQPRGICVWHPHSPTETEAW
                     RFFLVDADAPAEVKDVLRRFYMRYSGPAGMTEQDDMENWLYATAASKGTIARRYPFNY
                     QMSMGAYKTDDPVPGDVATQITEQTPRNFYRVYASYLNGDGWDALIGRRPGPVRMAAE
                     "
     gene            complement(2913..3647)
                     /locus_tag="VFN42_06335"
     CDS             complement(2913..3647)
                     /locus_tag="VFN42_06335"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET8996268.1"
                     /translation="MQPDHIYLPPMGGISIRQPDTQPGQATLSSAVYARLRRDILLGV
                     FRPGDKLPIDALCARYAIGATPLREALNRLSAEELVVRADQRGFRVAPVSLSDLEELT
                     KTLCWIGNLALRESIRHGDAAWEEAIVLAAHRLSRVPREGPEGYSSFNPEWETRHRAF
                     HLALIAACGSHWLIDFYAMLLDRNTRYRYLAFADASKPRDAEAEHRDIVEAVLARDAD
                     RAAAAAEAHMRRTSDTVARMFSPARA"
     gene            3718..4497
                     /locus_tag="VFN42_06340"
     CDS             3718..4497
                     /locus_tag="VFN42_06340"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8996269.1"
                     /translation="MAGATTQRLAVCDTPLETLRCGSGHAVVALHGMQTIDPDARFLE
                     LLARRTEVIAPTHPGFGDSPRPDSCETVYDLVHLYLGLLDSLPHDKVTLLGFSFGGWL
                     AAEIAAASCHRIDRLILVAPFGIKLGDRETRDITDVFNTHPDEVRRRAWHDPDRFAPD
                     YNAMEDAALIRRARSWDALCLYGWHPYMYNPRLKHWLRRISVPTQVVWGASDRIVTPE
                     YGRGYAALIPDARFTMIERAGHHPEIEQPDAFADHVLGFIG"
     gene            4508..5731
                     /locus_tag="VFN42_06345"
     CDS             4508..5731
                     /locus_tag="VFN42_06345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8996270.1"
                     /translation="MEAWWQCEIPYPYVDKGVLDAADSVRGSLPNRYCDPRIAADLFE
                     EVLDEFLLCDAVGMNVLAIEHHAGINSLMGSNPMFVSILARQTRQARILSLGTLVSLR
                     KDPVRIAEEYATADVMSRGRLEIGFVKSGGTEMASANTNPVGNVERYWEAIDLIRKTL
                     TSHDGPFSWEGKYYTHRHVNIWPPPWQRPHPRLWSATGDPETAAEVGRRGMVHVLVLR
                     GPDGTKRAYAAHRRARAEAGLPKVTTDNFAYAALVYVGDTEEEGRQIGSKLLWFLNTS
                     LKSAPQYSKFLPGTVPPQFAPDVYRTAPRPAPAEPNLVNAEKGVTPASQNARKLMSIT
                     TDEAMQQGILFAGNPDSVYRQIMEFYDKVGGFGHLAMIGRSGFMTHAESEKGIRLFAK
                     EVLPRLKAVAPVEVG"
     gene            5907..>6943
                     /locus_tag="VFN42_06350"
     CDS             5907..>6943
                     /locus_tag="VFN42_06350"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HET8996271.1"
                     /translation="MPLEHVPPDAVLPIAERRMLGRQLRKIVPRSQQASWHVPPSRRD
                     PVQMLVENGRHLLSHLVPLRYHRMRSSAFAFMRGSAAVMAADLAHTPSCGLMVQSCGD
                     CHFANFGVFAAADGTPVFDLNDFDETLRAPFEWDLKRLATSFAVDARGRSLPDRVCRH
                     LTRVLVQSYRVQMSALATLDPLSAWHHRIDVLDLLNRIDDPKLRAREVRRLQAATEAG
                     RRGYPRLLHRQKGAWRIRPRPPLVVPLTGQTDDTHEIAARAALESYLTTVGPALRLLL
                     GRYRLTDLAFKVVGIGSVGSFCAIGLFATSEGATLLLQLKEARASVLAPFTGAGCVGN
                     QGQRVVVGQRI"
BASE COUNT         1143 a         2362 c         2259 g         1179 t
ORIGIN      
        1 caatttcggc atgcagttca acggcagcct gtttgacgtg ctgagctggg cgatcatcaa
       61 gggcagcccc aaccggcaga acgcggagca gttgctgtat ttcgccggca ccccgggcat
      121 cgaggcgcag ttgttccgcg ccaccggcga ggcggggctg gccaagggtg ccaatgacgg
      181 cctgtcggcg aacctgctgc cgatgttgcc cacgatgcag aacaacctcg gtgccgccct
      241 gcgcatcgac accgcgttct ggcaccagaa cctggccaag ctgcagaagc gcttcgatag
      301 ctggctgaac gcgcactgac gcgctgctcg tcatggccgg ggaaggccgg ccactcatga
      361 cgtgcgggcc tagtcacgca tgaaaccagg cggtgatgat gccgaccctt gtgtcatgcc
      421 cgtgcctggc acgggcaccc acgtctttct cgggcgcagc agcaggaagc cgtggctggc
      481 cgcgccacgc gcggccatga cacgtcgttg gccaatctga attacgccaa ctcaatcacg
      541 tcgctggcgt ggcacagttc ctcgctgccg accgccgcac tggcgccctc tggcacctgc
      601 agcagccgca cgcgcagcca ttcgccgtcg cccatgttct ccagcacctc gacaaccagt
      661 ccgccggcga tgcgcagttt ggtgcccggt gtgatgtcgc tgacaacgat catgatcaga
      721 acaggatgct gagattcttc gcgagcagca cattctgctc caggatcagc tcgcgccggg
      781 caatctgcca ggactcaccc gcgcggcgca gcacgtcggt gcggcggccg atgaagctat
      841 aggtctcgta ttccacccgg ttctgatgca ccaggaagcg gcaacccacc gtcacctgct
      901 gcggcgcttc ggccgagggc tgcacatcca gcagccgcac attgctcacc atgtgcgtca
      961 cgcgcgacag cggttcctcg gcgtagtgca cgccggtcag gatctgctcg acccgcttgg
     1021 tgagagtcca cttgtcttcg tcgaaccagc tgatgccttc gccctggcgg gtattctcgc
     1081 gctcttcgtg ctggccgaac ttcacgttgc ggcggatcgg catgaaatac accaggtcgt
     1141 ccgccagcag atccagccac tcggtgaagc ggcgctggtc cagcaggtcg gcttcgccgt
     1201 acaggaagtc ttcgatctcc tgcttcagtt cgaaccaggc caggccgcgg cgcaccggcc
     1261 tgcgttcgat cacctcggtc atgcgcgcta ctccgccgcc atccgcaccg ggccgggcct
     1321 gcgcccgatc agggcgtccc agccgtcgcc gttcaggtag ctggcataga cgcgatagaa
     1381 attgcgcggc gtctgttcgg tgatctgcgt ggcgacgtcg ccgggcaccg gatcatccgt
     1441 cttgtacgcg cccatcgaca tctggtagtt gaacggatag cggcgcgcga tggtgccttt
     1501 gctggccgcc gtggcgtaga gccagttctc catgtcgtcc tgctccgtca tcccggcggg
     1561 gccggagtag cgcatgtaga agcggcgcag cacgtccttc acctcggccg gtgcgtcggc
     1621 atccaccagg aagaagcgcc aggcctcggt ctcggtcggg ctgtgcggat gccacacgca
     1681 gatgccgcgc ggctgccggc catggtacga cgcattgggg aacaggttgc cgacgaaggg
     1741 cagcaggcgc gccttgtcgc ccagccggcg ctggcgttca tggaagcagt ggcggaaata
     1801 ttcgtccacg atcggataat gctggtaggt cggctcgtac tgcatgtcct ccggcatcag
     1861 cacgctgtgc atgccgtgcc cctgcgggaa gccgacccag atgtgctggc cttggttgta
     1921 ttcgttgtcg cgccgcccct tgatcccccg ctgtccgctg gggttgatgc cgatcatgtc
     1981 caccgagcgg tgtgacggat tgtggtaggt gtcaccgagg aagttctctg ccgcgaactt
     2041 ccagttcgcc ggaaagatcc atttgtgcac gccgatcact tccgatccgc cgggccgccc
     2101 gtcacggcag tccagcacct ggtccaggtg atccaccgcg ccgccgagat actccatgaa
     2161 gtcgggcgcc tgcggatccc aggatgccca caccgtgccc ttgtacagcg ccagcttcgg
     2221 cacctcgatc agcgaccact cttcgcgctt cagcgtgttc tcatacagca cgcggtacag
     2281 cggcacgcct tgcagcttgc cgtcggtggt gtagctccag gaatggtacg ggcagacgaa
     2341 cagcgaggta ttgccctgtt cgtagcggca gaccttcatg ccgcgatggc ggcaggaatt
     2401 caggaacacg tgcaccgcac cctgagcgtc gcggcacagg atcaccgatt cctcgcccat
     2461 gcgggagacg aagaagtcgc cgggcttggc aatctggctt tcgtgtccga tgaacagcca
     2521 ggcgcgcgtg aacagcttct ccagttcggc gcggtggtag tccggagaga cgaaaatctc
     2581 ccggctgatc atgccgtgtt ccagatcgat cgcctgatcc agcggcagtt gcgtggccat
     2641 gcggagtcct ccctccgtcg cggcgcgttt ccgccgcggt tgccgcattc tgcccatcgg
     2701 ggggctggcg gcgtcaagcc cggttcatac cggcggcagt ctctcgtcgt ggcggacgca
     2761 ggcccgccac ccacaacttt gctgcgcgca actgaggaaa gtcggggata ccggccttcg
     2821 ccggaaagac ggaattcggc gtgccttcgc cggcatgacg gaattcgacg tgccgaagag
     2881 tcgagctgat cgcgggttgg tatcagtcgc ggctaggcgc gggcaggcga aaacatccgc
     2941 gccactgtat ccgacgtgcg ccgcatatgc gcttccgccg ccgccgcggc gcggtcggca
     3001 tcgcgcgcca gcaccgcctc gacaatgtcc cgatgttccg cctcggcatc gcgcggcttc
     3061 gacgcatcgg cgaaggccag gtagcgatag cgcgtgttgc ggtccagcag catcgcgtag
     3121 aagtcgatca gccaatgcga gccgcaggcg gcaatcagcg ccagatggaa cgcgcggtgc
     3181 cgggtttccc attccggatt gaacgaactg tagccctcgg gcccctcgcg cggcacgcgg
     3241 gacaggcgat gggcggccag cacgatcgct tcttcccacg ccgcgtcgcc atgacggatg
     3301 gattctcgca gtgccaggtt gccgatccag cacagcgtct tggtcagttc ctccaggtcg
     3361 gacaggctga ccggcgcgac acggaacccg cgctggtcgg cgcgcaccac cagttcctcc
     3421 gccgacagcc ggttcagcgc ctcgcgcagc ggtgtggcgc cgatcgcgta gcgtgcgcac
     3481 agcgcatcga tcggcagctt gtcgcccgga cggaacacac ccagcaggat gtcgcggcgc
     3541 aggcgcgcat agaccgcgct ggacagggtc gcctgcccag gctgcgtgtc cggctgccgt
     3601 atcgaaatcc cgcccatcgg gggcaaatat atatgatcgg gttgcatgac gccaaaccta
     3661 tatgctctgc tgcgcgccag caggggcagc cgcgatggcc cccgggagga accatccatg
     3721 gcaggcgcaa cgacgcaacg gttggcggtc tgtgacaccc cgctggaaac cctgcgatgc
     3781 ggcagcggac acgcggtggt cgcgctgcac ggcatgcaga ccatcgatcc ggacgcccgc
     3841 ttcctggagc tgctggcccg ccgcaccgaa gtgatcgcac cgacccatcc gggctttggc
     3901 gattcacccc ggccggactc ctgcgagacg gtgtacgacc tcgtgcatct ctatctcggc
     3961 ctgctcgact ccctcccgca cgacaaagtt accctgctcg gcttttcctt cggcggctgg
     4021 ctcgccgcgg aaatcgcggc ggcgagctgc catcgcatcg accggctgat cctggtggcg
     4081 cccttcggca tcaagctggg cgaccgggag acgcgcgaca tcaccgacgt gttcaacacg
     4141 catcccgacg aggtgcgccg ccgagcgtgg cacgatccgg atcgcttcgc gcccgactac
     4201 aacgcgatgg aagacgccgc gctgatccgc cgagcgcgaa gctgggatgc gctgtgcctg
     4261 tacggctggc atccctacat gtacaacccg cggctgaaac actggctgcg tcgcatcagc
     4321 gtgcccaccc aggtggtgtg gggcgcatcc gaccgcatcg tcacgccgga atacggccgc
     4381 ggctacgcgg cgctgatccc cgatgcgcgc ttcaccatga tcgagcgtgc cggccatcat
     4441 ccggagatcg agcagccgga tgccttcgcc gaccacgtcc tcggcttcat cggataggag
     4501 accggccatg gaagcctggt ggcagtgcga aatcccctat ccctacgtcg ataagggcgt
     4561 gctggacgca gcggattcgg tgcgcggcag cctgccgaac cgctattgcg atccgcgcat
     4621 cgccgcggac ctgttcgagg aggtgctgga cgagttcctg ctgtgcgatg ccgtcggcat
     4681 gaacgtcctg gcgatcgagc accatgccgg catcaactcg ctgatgggct ccaacccgat
     4741 gttcgtcagc atcctggcgc gccagacgcg gcaggcgcgc atcctcagcc tgggcacgct
     4801 ggtgtcgctg cgcaaggacc cggtgcgcat cgccgaggag tatgcgacgg cggacgtgat
     4861 gtcgcgcggc cggctggaga tcggcttcgt caagtcgggc ggcacggaaa tggcgtcggc
     4921 caacaccaat ccggtcggca acgtggagcg ctactgggaa gccatcgatc tgatccggaa
     4981 gacgctgacc agccatgacg gtccgttcag ctgggagggc aagtactaca cgcatcgcca
     5041 tgtgaatatc tggccgccgc cctggcagcg cccgcatccg cgcctgtggt ctgctaccgg
     5101 cgatccggaa accgcggcgg aagtcggccg gcgcggcatg gtgcatgtgc tggtgctgcg
     5161 cggcccggat ggcacgaagc gcgcctatgc cgcgcatcgc agggcacgtg ccgaggcggg
     5221 gttgcccaag gtcaccacgg acaatttcgc ctatgcggcg ctggtgtatg tcggcgacac
     5281 cgaggaggaa ggccggcaaa tcggcagcaa gctgctgtgg ttcctcaaca ccagcctgaa
     5341 atcggcgccg caatactcga agttcctgcc cggcaccgtg ccgccgcagt tcgcgcccga
     5401 cgtctaccgg accgcgccgc gtcccgcacc cgccgaaccc aacctggtga atgcggaaaa
     5461 gggcgtcacg ccggcaagcc agaatgcacg caagctcatg tcgatcacca ctgatgaagc
     5521 gatgcagcag ggcatcctgt tcgccggcaa tccggacagc gtctatcgcc agatcatgga
     5581 gttctacgac aaggtcggcg ggttcgggca cctggccatg atcggccgct ccggcttcat
     5641 gacgcatgcc gagtccgaaa agggcatccg gctgttcgcc aaggaagtgc tgccgcggct
     5701 gaaggcggtg gccccggtgg aggtcggcta ggccagcggc ggccagctcc aacgccatgc
     5761 ggacagcgtg atgcccctgg gtctgatgca tcatgcatga cagttggacg acaatgcatc
     5821 cgatcggcgt cgccaggcgt gttctcgctt gcacgggacg ccggctttcg ttctgatccg
     5881 cagcgtccag cgcttcggag cagcccatgc cgctcgagca cgtcccgccc gacgccgtgc
     5941 tgccgatcgc ggagcggcgg atgctcggcc gccagttgcg caagatcgta ccgcgcagcc
     6001 agcaggcgtc ctggcacgtg ccgcccagcc gtcgcgaccc ggtgcagatg ctggtggaga
     6061 atgggcggca cctgctctcg cacctggtgc cgctgcgcta ccaccgcatg cgcagctccg
     6121 ctttcgcctt catgcggggc agcgcggcgg tcatggcggc ggacctcgcg catacgccaa
     6181 gctgcggatt gatggtgcag tcctgtggcg actgccattt cgccaatttc ggggtgttcg
     6241 ccgccgcgga cggcacgccg gtgttcgacc tcaacgactt cgacgagacg ctgcgcgcgc
     6301 cgttcgaatg ggacctgaag cgcctggcca ccagcttcgc ggtggacgca cgcgggcgca
     6361 gcctgcccga ccgggtctgc cgccatctga cccgcgtgtt ggtacagtcc taccgcgtgc
     6421 agatgagcgc cctcgccacg ctcgatccgc tgtccgcctg gcatcaccgc atcgacgtgc
     6481 tggacctgct gaaccgcatc gacgacccga agctgcgcgc ccgcgaagtc cggcggctgc
     6541 aggcggcgac ggaggcgggc cgccgcggct atccgcggct gctgcaccgg cagaaaggcg
     6601 cgtggcgcat ccggcctcgc ccgccgctgg tcgtcccgct gaccggccag accgacgaca
     6661 cgcacgagat cgccgcgcgc gccgccctcg aatcctacct gaccacggtc ggccccgcgc
     6721 ttcgcctgct gctcgggcgc taccggttga ccgacctggc gttcaaggtc gtcggcatcg
     6781 gctcggtggg cagcttctgt gccatcggcc tgttcgccac cagcgaaggc gccaccctgc
     6841 tgctgcagct gaaggaggcg cgcgcctcgg tgctggcgcc gtttaccggc gcaggctgcg
     6901 tcggcaacca gggccagcgc gtggtggtcg ggcaacggat cat
//
LOCUS       DASSZH010000295         2185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_382202, whole genome shotgun sequence.
ACCESSION   DASSZH010000295 DASSZH010000000
VERSION     DASSZH010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382202"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VFN42_06355"
     CDS             complement(<1..251)
                     /locus_tag="VFN42_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrB/QacA family drug resistance transporter"
                     /protein_id="HET8996272.1"
                     /translation="MSGEVHAAFHSRGSPWVIAIIVTMAPFMEILDTTIVNVSLPHIA
                     GALSASYDDSTWTLTSYLVANGIIVPVSGWIGRVLGRKQ"
     gene            complement(248..1372)
                     /locus_tag="VFN42_06360"
     CDS             complement(248..1372)
                     /locus_tag="VFN42_06360"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HET8996273.1"
                     /translation="MNDQPIPSGRPATSAPPPTRQPEPAAPPPRRGAARARWVLLLIL
                     LAALGAGAAYWYLTRNQVTTDDAFTDGNAVMVAPQVSGTVVALEVSDNQPVTAGQVLL
                     KIDPRAYQAARDEAAGNLAVAHAQLADAQARLASARIVFPARLADAQAQLAAAQATEF
                     KARTDLRRQQHLPKQATTQQALDDAEAAMRSADAQVAQARASLRSADTVQQSVEQAEA
                     QVRQLQGQVELAKARLAQAQLNLDWTVLRAPQAGWVTKRNVNQGNYVTPGQALMSLVT
                     SQVWITANFKEADLARMRRGQKVAISVDAYPSLHLEGHVDSIQLGSGSRFSAFPVENA
                     TGNFVKVVQRVPVKIVIDSGLDPNIPLPLGLSVEPTIYLK"
     gene            1444..>2185
                     /locus_tag="VFN42_06365"
     CDS             1444..>2185
                     /locus_tag="VFN42_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET8996274.1"
                     /translation="MSYEHILTEVEDGVAILTLNRPDKLNAMNRKLSAELHHAVKRME
                     ADDAVGCIVITGAGERAFSAGGDIHEQLADDARYSDEELAAMRSGRRSYEIAACAKPT
                     IGMMNGLAYGGAAVLASSLDMRIGCEASRFRFLAAAYGRINSTWTLPNQVGWPMAKEL
                     LFSARVVEAEEAYRIGLLNHLVPRRELRSRTMELARTIAGNHRGAVMGIKQLLLQQLG
                     GDLSHQWQAEHDFAASEFRNAKAEEAFPE"
BASE COUNT          332 a          708 c          799 g          346 t
ORIGIN      
        1 tactgcttgc gtcccagcac ccggccgatc cagccggata ccggcacgat gatgccgttc
       61 gccaccaggt aggaggtgag cgtccaggtg gaatcgtcgt agctcgccga cagcgcgccg
      121 gcgatatgcg gcaggctgac attgacgatg gtggtgtcca ggatctccat gaacggcgcc
      181 atcgtcacga tgatggcgat cacccagggg ctgccccttg agtggaaggc ggcgtgaacc
      241 tcgccgctca tttcaggtag atggtgggct cgaccgacag gcccagcggc aggggaatgt
      301 tcgggtccag cccggagtcg atgacgatct tcaccggcac gcgttgcacc accttcacga
      361 aattgccggt ggcgttctcc accggaaatg ccgagaagcg cgagccggag ccaagctgga
      421 tgctgtccac gtgcccttcc aggtgcagcg agggataggc atccacgctg atggccacct
      481 tctggccacg ccgcatgcgg gcgagatcgg cctccttgaa attggcggtg atccacacct
      541 gggaggtgac cagcgacatc agcgcctggc ccggggtgac gtaattgccc tggttgacgt
      601 tgcgcttggt cacccagccg gcctgcggcg cgcgcagcac ggtccagtcc aggttgagct
      661 gggcctgggc cagccgtgcc ttggcgagct ccacctggcc ctgcagctgg cgcacctgcg
      721 cctcggcctg ctcgaccgac tgctgcacgg tgtcggcgct gcgcaggctg gcccgggcct
      781 gcgccacctg ggcgtcggcg gagcgcatgg ccgcttcggc gtcgtccagc gcctgctgcg
      841 tggtggcctg cttcggcaga tgctgctgcc ggcgcaggtc cgtgcgtgcc ttgaactcgg
      901 tggcctgggc ggccgcgagt tgcgcctggg cgtcggccag cctggcggga aacacgatgc
      961 gggcgctggc cagccgagcc tgggcgtcgg cgagctgggc atgggcgacg gcgaggttgc
     1021 cggcagcctc gtcgcgcgcc gcctggtagg cgcgcgggtc gatcttcagc agcacctggc
     1081 cggcggtcac cggctggttg tcgctgacct ccagcgcgac caccgtgccg gacacctgcg
     1141 gtgccaccat caccgcattg ccgtcggtga aggcgtcgtc ggtagtgacc tggttgcggg
     1201 tgaggtacca ataggccgcg ccggccccca gcgcggccag caggatcagc agcagcaccc
     1261 accgggcgcg ggcggcgccg cggcggggcg gaggtgcggc aggctcgggt tgccgggtcg
     1321 gcggcggcgc ggaggttgcg gggcggccgg acggaatggg ctggtcgttc atcgctgccg
     1381 caccttattg ccgctgcgat ccagacgaca atagactgcc ggcgatccgg ggagcgcagt
     1441 ccaatgtcct acgagcacat cctgaccgag gtggaagacg gcgtcgccat cctgacgctc
     1501 aatcgccccg acaagctgaa cgcgatgaac cgcaagctgt cggccgaact gcatcacgcg
     1561 gtgaagcgga tggaggcgga tgacgcggtc ggctgcatcg tcatcaccgg cgcgggagag
     1621 cgggcttttt cggccggtgg cgatatccac gaacaactgg cggacgatgc gcgctactcc
     1681 gacgaggagc tggccgccat gcgcagcggg cgccgcagtt atgagatcgc cgcgtgtgcc
     1741 aagcccacca tcggcatgat gaacggcctg gcctatggcg gcgcggcggt gctggcctcg
     1801 tcgctggaca tgcggatcgg ctgcgaggcg tcccgcttcc gcttcctggc ggcggcctat
     1861 gggcggatca acagcacctg gacgctgccg aaccaggtcg gctggccgat ggcgaaggag
     1921 ctgctgttct ccgcccgcgt ggtcgaggcg gaggaggcct accgcatcgg cctgctgaac
     1981 cacctggtgc cgcggcggga gctgcgcagc aggacgatgg agctggcgcg caccattgcc
     2041 ggcaaccacc gcggcgcggt gatggggatc aagcagctgc tgctccagca gcttggcggg
     2101 gatctgtcgc atcagtggca ggcggagcat gacttcgccg cctccgaatt ccgcaatgcc
     2161 aaggcggagg aggcgttccc ggagt
//
LOCUS       DASSZH010000296         5783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_705914, whole genome shotgun sequence.
ACCESSION   DASSZH010000296 DASSZH010000000
VERSION     DASSZH010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705914"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VFN42_06370"
     CDS             complement(<1..288)
                     /locus_tag="VFN42_06370"
                     /inference="COORDINATES: protein motif:HMM:NF023338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3429 domain-containing protein"
                     /protein_id="HET8996275.1"
                     /translation="MPLSVLLLGLIGLAPFIGCGLAAIGPHASGSDPWLAALIGYAAV
                     VLGFVGGVHWGLVLREPEPQQQQTTRIGLAVVPLVLGWAALVSAATVAYWVA"
     gene            810..1616
                     /gene="panB"
                     /locus_tag="VFN42_06375"
     CDS             810..1616
                     /gene="panB"
                     /locus_tag="VFN42_06375"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HET8996276.1"
                     /translation="MSTQVEQRRITVRHFRKGGGPYACLTAYTAPVARLLDPHVDLLL
                     VGDSLGMVVYGMDTTVPVTLDMMIAHGAAVVRSTRHALVVVDLPFGSYQESPQQAFRN
                     AARVLAETGATAVKLEGGVDMAETIRFLTRRGIPVCGHIGLMPQAVNASGFRAQGRNE
                     QEAAAIAADAQAVAEAGAFIMVIEGTYRALASRITQSVPIPTIGIGASPDCDGQILVT
                     DDVLGLFGTFTPRFVKRYAELAGTVSAAAERYARDVRARAFPAPEHCFGD"
     gene            1691..2641
                     /locus_tag="VFN42_06380"
     CDS             1691..2641
                     /locus_tag="VFN42_06380"
                     /inference="COORDINATES: protein motif:HMM:NF006045.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HET8996277.1"
                     /translation="MDRVEHGREKYQLVLAMARRQPPVVTAVVHPCDAVSLAGAVEAH
                     RLGLIEPILVAPAGRLEAIAAASGIDITGLPQVASEHSHDSAAKAVALVREGKADALM
                     KGSLHTDEVMGAVVAHDSGLRTERRISHCFIMDVPGHAAPLIITDAAVNIAPTLEDKR
                     DIVQNAIELAHAIGLDRPLVAILSAMETVNPKVPSTVEAAALCKMADRGQITGGVLDG
                     PLALDNAISPEAAAIKHIVSPVAGHANVLVVPDLEAGNMLAKSLSFLADAESAGIVLG
                     ARVPIILTSRADSLVSRLASCAVAALVAAWRKRQAAEAVR"
     gene            2726..3775
                     /locus_tag="VFN42_06385"
     CDS             2726..3775
                     /locus_tag="VFN42_06385"
                     /inference="COORDINATES: protein motif:HMM:NF021423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2125 domain-containing protein"
                     /protein_id="HET8996278.1"
                     /translation="MRRGFRWRSLGLSAFALLVLLAAGDAVYWRIVAGQLDSGFRAWI
                     AAQRAAGWTVRGTPPRFGGWPLAATLTTTRISLSGGKLDFPGGMDWDAGRLVLRVALL
                     HPEQLQAIPSGVQHLRLGTGPAIPFTAGTMQAVVPLQPDQPPRTVILHATDLRAGLAG
                     GGSTFTIGRLQAQLDSQPSAAKPALGFAIQATSLALPGQYRWALGPSIEAVTLDGTLT
                     GPFPATPDLTARATAWRDGGGALELRRLHLNWGPLSLTANARLGLDQQLQPAGHAEAR
                     VAGYDATLDALATAGALSPSAALAAKAVLSLMAQPAANGKPAEVDVPLRLQNGTLFMQ
                     TMPLVRLPVLDWPLP"
     gene            3892..4182
                     /locus_tag="VFN42_06390"
     CDS             3892..4182
                     /locus_tag="VFN42_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996279.1"
                     /translation="MPSDLPAAACDSVAVRFMLDAEASPGLLPRLIQPFARRDLMPDR
                     MWSHRNGATMHVEIAVEAMPAEMVHVVEGNLRQIVGVHRLSRLHPPELRQVA"
     gene            complement(4473..>5783)
                     /locus_tag="VFN42_06395"
     CDS             complement(4473..>5783)
                     /locus_tag="VFN42_06395"
                     /inference="COORDINATES: protein motif:HMM:NF006543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan -binding protein"
                     /protein_id="HET8996280.1"
                     /translation="AQGFLSAALTGINKALDKVNAQLAQLTDALSLERAHAGQLQLSI
                     AQLNKQLSGANAARDTLSQQLQAAQEQVQQALAAADKLRTERTRLTEQLSDANQVAQS
                     QAAANQKLQTQLAAATSQADTEGQQNAKVTAQLADVQQQLQAAKQQLADMQQQMAALN
                     KTVSVDKDTIKTKLSDLAKLSEQVQALTALRDQLEKQAQDAAVQATTEKQRREAVAAE
                     LAKEKKLGDSAQAQIALLNKQVEQLKDQLASVAQSLDIAEKQGRDKDVQIANLGQKLN
                     AALAAKVQELQSYRSEFFGQLRKVLAGRSGIKVVGDRFVFQSDVLFPVGSANLTDNGV
                     AQLTALAVQIKDLIPEIPKDLHWVLEVDGFTDPTPVKGGQFASNWELSTARALSVVKL
                     LIQDGIPPHHLAAAGFGEYQPVVPGNTPEDYAKDRRIELRLTSG"
BASE COUNT          838 a         2073 c         1938 g          934 t
ORIGIN      
        1 cgccacccag tacgccaccg tcgccgcgct gaccaaggct gcccagccaa gcaccagcgg
       61 caccaccgcc aagccgatgc gggtggtctg ctgctgctgc ggttctggct cgcgcagcac
      121 caggccccaa tggaccccgc cgacgaaccc cagcaccacc gcggcatagc cgatcagggc
      181 cgccagccag ggatccgagc cgctcgcgtg cgggccaatc gccgcgaggc cgcagccgat
      241 gaacggtgcc aggccgatca ggccgagcag aaggacggaa aggggcatgg cggttcctgg
      301 tgtctccggc gcatgccgca tgatgcccgc actgccgcct ggaggccatc ggcaccggcc
      361 gccgtggcac caatcctgcg cccggccgga aagaaacccg ctggcgattg atatagatca
      421 gtgagccctc cccgccgctc aggctaggtg agcggacttc ctcccatact tctcctctgt
      481 cactgccccg gccccctccc aggagccggg gacttttttt tgcccggaac gccggggcac
      541 atcggcgcag cacgggcaac tgcccgcgcc cgcacgggtt aaccgcctgc ccggtccgta
      601 attcgacctt acggtgcggg tggcgtggcc cgcctgagag atttggcggg ccgcgtcccg
      661 gtctgccggg agcggcgccg ccggccggtt ttccgcggca tggcaggtcg acgcggaacg
      721 caatgcgcaa ccgccccagg ccgctgtccc cgccatcacc gccggcctat gcacgcgcgc
      781 aggcacccgc ccgggtcgag gagagatgga tgagcaccca agtcgaacag cgccgcatca
      841 ccgttcgcca ttttcgcaag ggcggcggcc catacgcctg cctgaccgcc tacaccgccc
      901 cggtcgcgcg gctgctcgac ccgcatgtgg acctgctgct ggtcggcgat tcgctgggca
      961 tggtggtgta cggcatggac accacggtgc cggtaacgct cgacatgatg atcgcgcacg
     1021 gcgccgcggt ggtgcgcagc acgcggcatg cgcttgtggt ggtcgatctg cccttcggca
     1081 gctaccagga atccccgcaa caggccttcc gcaatgccgc acgggtgctg gccgaaaccg
     1141 gcgccaccgc ggtgaagctg gagggcggcg tcgacatggc ggagaccatc cgcttcctca
     1201 cccggcgcgg cattccggtc tgcggccata tcgggctgat gccgcaggcg gtgaacgcct
     1261 ccgggttccg ggcgcaaggc cgcaacgagc aggaagccgc cgccattgcc gccgacgctc
     1321 aggccgtggc ggaggccggc gccttcatca tggtgatcga gggcacctat cgcgccctcg
     1381 cctcccgtat cacccagtct gtgccgatcc ccaccatcgg catcggcgcc tcgccggatt
     1441 gcgacgggca gatcctggtc accgatgacg tgctcggcct attcggcact ttcaccccgc
     1501 gcttcgtgaa gcgctatgcc gaactcgccg gcaccgtcag cgccgccgcc gaacgctatg
     1561 cgcgcgacgt gcgggcgcgc gccttccccg cgccggagca ttgcttcggc gattgagctt
     1621 gatctcgctc aaggctccgc cgcgcgccag ttcctagagt tgcccgcggg cgaacaggga
     1681 aaggcgggcg atggaccggg tggagcacgg acgggagaag taccaactgg tcctggcgat
     1741 ggcgcgccgc cagccgccgg tggtcaccgc cgtggtgcat ccctgcgacg cggtgtcgct
     1801 ggccggggcg gtggaagccc accggctggg cctgatcgag cccatcctgg tcgccccggc
     1861 cggccgcctg gaggcgatcg ccgccgccag cggcatcgac atcaccggcc tgccgcaggt
     1921 cgcctcggaa cacagtcacg attccgcggc caaggcggtc gccctggtgc gggagggcaa
     1981 ggcggacgcg ctgatgaaag gcagcctgca caccgatgag gtgatgggcg cagtggtcgc
     2041 gcatgacagc ggcctgcgca ccgagcgccg catcagccac tgcttcatca tggacgtccc
     2101 cggccatgcc gcgccgctga tcatcaccga cgccgcggtg aacatcgccc cgacgctgga
     2161 ggacaagcgg gacattgtgc agaacgccat cgagctggcg catgcgatcg gcctcgaccg
     2221 gccgctggtc gccatcctct cggccatgga gacggtgaac ccgaaagtgc cctccacggt
     2281 cgaggcggcg gcgctttgca agatggcgga tcgtggccag atcaccggcg gggtgctcga
     2341 cggcccgctg gcgctggaca atgccatcag cccggaggcc gcggcgatca agcacatcgt
     2401 ctcgccggtg gccgggcatg ccaacgtgct ggtggtgccg gacctggaag ccggcaacat
     2461 gctggccaag agcctgtcct tcctggcgga tgccgagtcc gccggcatcg tgctgggcgc
     2521 gcgggtgccg atcatcctga ccagccgagc ggattcgctg gtcagccggt tggcatcctg
     2581 cgcggtggcc gcgctggtgg ctgcctggcg caagcggcag gcggccgaag cggtgcgctg
     2641 agccgcggct tcgttgcggc ccgcttgcct tcgcgcggcc acgccggctg cctataagcg
     2701 ccgcgcaacc gatggcagga atggcatgcg gcgcggcttc aggtggcgga gtcttggact
     2761 gtcggcgttc gcgctgctgg ttctgctggc ggcaggcgat gcggtgtatt ggcgcatcgt
     2821 ggccgggcag ctcgattccg gcttccgcgc ctggatcgcc gcgcaacgcg cggcaggctg
     2881 gacggtgcga ggcaccccac cccggttcgg tggctggccg ctcgcggcga cgctcaccac
     2941 cactaggatt tcactgtccg gtggaaagct cgatttcccc ggcggcatgg actgggacgc
     3001 cggccggctg gtgctgcggg tcgccctgct gcacccggaa cagttgcagg cgatccccag
     3061 cggcgtgcag cacctccgcc tgggcaccgg acccgccatc ccctttaccg ccgggaccat
     3121 gcaggcggtg gttccgttgc agccggatca accaccgcgc acggtcatcc tgcatgccac
     3181 cgatctgcgc gccggcttgg ccggcggcgg aagcacgttc accatcggca ggcttcaggc
     3241 ccagctcgac agccagccgt ccgcggcaaa accggcgctc ggcttcgcca tccaggccac
     3301 ctccctggca ctgcccggac agtaccgctg ggcgctcggc cccagcatcg aagcagtgac
     3361 gctggacggc acgctgaccg gccccttccc cgccacgccc gaccttaccg cccgcgcaac
     3421 cgcgtggcgc gacggcggtg gcgccctgga acttcgccgg ctgcacctga actggggacc
     3481 gctgagcctg acggcgaacg cgcggctggg tctcgatcaa caactccagc cggccggcca
     3541 cgccgaggcc cgggtggccg gttacgacgc cacgctggac gcgctggcca cggccggcgc
     3601 gctgagcccc tccgccgcat tggccgccaa ggcggtgctg tcgctgatgg cgcagccggc
     3661 ggcgaacggc aaacctgccg aggtggatgt gccgctccgg ctgcagaacg gcacgctttt
     3721 catgcagacc atgccgctgg tacggcttcc ggtactggac tggccgctgc cctgaggcgt
     3781 ataagcggca catgcatctc tggaccgaca ggacgcgaat tatcggcccg gctgcctgag
     3841 cagccggggc tgatcacccg tccttcgcgt tattgtccgg agtcgttccc catgccatcc
     3901 gatctgcctg ccgccgcgtg cgacagcgtc gccgtccgct tcatgctcga cgccgaggcc
     3961 tcgccgggcc tgttgccgcg cctgatccaa cccttcgccc ggcgcgacct gatgcccgac
     4021 cgcatgtggt cgcaccgcaa cggcgccacg atgcacgtgg agatcgccgt ggaagccatg
     4081 ccggcggaga tggtgcacgt ggtcgaaggc aatctgcgcc agatcgtcgg cgtgcaccgc
     4141 ctgtcccggt tgcatccgcc ggaactccgc caggtcgcct gaacggatat cgcgcgtgca
     4201 aatgaccggc ggtgccgtct gataccagcc cgccttgccg gtgacacacc gccagcagta
     4261 ccccgtcgtg gcgggcgaag ggggctgtaa tccaattggg cggacaggcc gggatgcagc
     4321 gtacaacgtc gtggcgggcg acggcccacc acccacgact tcgctgaacg cagctgagga
     4381 aagtcgtgga tgccggcctt cgccggcatg acggaggtcg gcacgccgaa gtgtcgggat
     4441 tatcgcgagc tggcatgcgt gcacgggaag cctcagccgc tggtcagccg cagttcgatc
     4501 cggcgatcct tggcgtaatc ctccggcgtg ttgcccggca cgaccggctg gtactcgccg
     4561 aaccctgccg ccgccagatg gtgcggcggt atgccgtcct ggatcagcag cttcaccacg
     4621 ctgagcgcgc gcgcagtgga cagttcccag ttcgaggcga actggccgcc cttcaccggg
     4681 gtcggatcgg tgaatccgtc cacctccagc acccagtgca ggtccttggg gatctccggg
     4741 atcaggtcct tgatctgcac cgccagcgcg gtcagttgcg ccacgccgtt atccgtcagg
     4801 ttggcgctgc ccaccgggaa caacacgtcg ctctgaaaca cgaagcggtc gccgaccacc
     4861 ttgatgccgg accgccccgc cagaaccttg cgcaactggc cgaagaactc gctgcgatag
     4921 ctctgcagct cctgcacctt cgcggccagc gccgcgttca gcttctggcc caggttggcg
     4981 atctgcacat ccttgtcgcg gccctgcttc tcggcgatgt ccagcgactg cgccaccgaa
     5041 gcaagctggt ccttgagctg ctcgacctgc ttgttcagca gcgcgatctg cgcctgcgcg
     5101 ctgtcgccca gcttcttctc cttggcgagc tccgccgcca ccgcctcgcg ccgctgcttt
     5161 tccgtcgtcg cctggacggc ggcatcctgg gcctgctttt ccaactggtc gcgcagcgcg
     5221 gtcagcgcct gcacctgctc cgacagcttg gcgagatcgg acagcttggt cttgatggtg
     5281 tccttatcga cgctgaccgt cttgttcagc gccgccatct gctgctgcat gtcggccagt
     5341 tgctgcttcg ccgcctgaag ctgctgctgc acgtccgcca gttgcgcagt gaccttggcg
     5401 ttctgctggc cttccgtgtc ggcctggctg gtcgcggccg cgagctgggt ctgcagcttc
     5461 tggttcgccg ccgcctggga ctgcgcgacc tggttggcgt ccgacagctg ctcggtcagc
     5521 ctggtgcgct cggtgcgcag cttgtcggct gcggccaggg cctgctgcac ctgctcctgc
     5581 gccgcctgaa gctgctggga cagcgtgtcg cgcgcggcat tggcgccgct gagctgcttg
     5641 ttgagctgcg cgatcgaaag ctgaagctgg ccggcatgcg cgcgttccag cgacagcgcg
     5701 tcggtgagct gcgccaattg cgcgttcacc ttgtccagcg ccttgttgat gccggtcagc
     5761 gccgccgaca ggaaaccctg ggc
//
LOCUS       DASSZH010000297         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_228752, whole genome shotgun sequence.
ACCESSION   DASSZH010000297 DASSZH010000000
VERSION     DASSZH010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228752"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="VFN42_06400"
     CDS             complement(<1..1015)
                     /locus_tag="VFN42_06400"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8996281.1"
                     /translation="MRQPPSKSTHPPSFPGHAASPTLPRPPGRRSLLRWFAASAALPV
                     PQAAGPVAFSLVAQSLTGEASGGAAMILAMTLAQVAGAIPITRFGRSLPAATVLRLLV
                     AFRALALAAMCLCVHARAPFAWLIVLAALAGLVNGAAAGYLRALLNHFTPSARLPRVL
                     GIAATLNEVTYVLGPVVASGLGAVSPVFAVLALTLLGAIPALLVPQAHSAHLGAAPHA
                     EASVLSLAILLWLGCAAAGGAMVAAIEIGSVSLALKFGYEPTLAILFTVPLCLASVTG
                     GIWVSVRNRMASRRSVVPKLSVMTLGGALVAFGSSVTVTAIGAVLIGIVLASLGTYYS
                     LMLD"
     gene            complement(1201..2079)
                     /gene="dapA"
                     /locus_tag="VFN42_06405"
     CDS             complement(1201..2079)
                     /gene="dapA"
                     /locus_tag="VFN42_06405"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET8996282.1"
                     /translation="MFKGSLVALITPMRADGSVDEKAYAELVEWQINEGTHGLVPVGT
                     TGESPTLSHDEHKRVVEICVEVAKGRVPVIAGAGSNSTEEAIGLTRHAKEAGADAALI
                     VTPYYNKPTQEGMYLHFKAIADAVDLPIIIYNIPPRSVIDMKVETMARLAKHKNIVGV
                     KDATADLARPLHQRRECGADFCQLSGEDATALAFNAAGGVGCISVTANVAPRLCSEMQ
                     TAWMEGRMQQAMEIQNRLLPLHDALFTETSPAPVKFAASLLGKSTEHCRLPLAPIMEP
                     TRAKVRAAMTEVGLLN"
     gene            complement(2192..>2466)
                     /locus_tag="VFN42_06410"
     CDS             complement(2192..>2466)
                     /locus_tag="VFN42_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter, permease protein
                     PstA"
                     /protein_id="HET8996283.1"
                     /translation="LPGVLTGVLLALAISVGETAPLLYTAGWSSYLWNGQLTHSPIGY
                     LTYAIWAFINEPFAAANELAYAAAFLTTGFVLLVSLAARLLLTRRT"
BASE COUNT          410 a          890 c          815 g          351 t
ORIGIN      
        1 ggtcgagcat cagcgagtag taggtcccca gcgaagccag cacgatgccg atcagcaccg
       61 ccccgatcgc cgtcaccgtc accgacgatc cgaacgcgac aagcgccccg ccgagcgtca
      121 tgacggacag cttcggcacg accgatcggc ggctcgccat gcgattgcgc acgctgaccc
      181 agatgccgcc cgtgacggat gccaggcaca gcggcacggt gaacaggatg gcgagcgtcg
      241 gttcgtagcc gaatttcagc gccagcgaga ccgagccgat ctcgatggcc gcgaccatcg
      301 cgccgcccgc cgcggcgcag cccagccaca gcaggatcgc caggctcagc accgacgcct
      361 cggcatgcgg tgcggcgccg agatgcgcgc tgtgcgcctg cggcaccagc aaggccggaa
      421 tcgcccccag cagcgtcagc gccagcacgg caaacaccgg ggagacggcg cccaacccgg
      481 acgccaccac cggccccagc acataggtca cctcgttcag cgtcgctgca atgcccagca
      541 cccggggcag ccgcgcgctc ggcgtgaaat ggttgagcag cgcgcgcaga tatccggccg
      601 cggcaccgtt caccagcccg gcgagcgccg ccaggacgat cagccaggca aacggcgcgc
      661 gggcatgaac acacaggcac atcgcggcga gcgccagcgc gcggaaggcc accagcagcc
      721 gcagcaccgt tgcggcaggc aggctccggc cgaaccgtgt gatcgggatc gcgccggcca
      781 cctgcgccag agtcatggcc aggatcatcg ccgcgccgcc gctcgcctcg ccggtcaggc
      841 tttgggcgac gagcgagaat gcgacggggc cggccgcttg cggcaccggc agagcggccg
      901 acgcggcgaa ccagcgcaac agcgaacggc ggcccggcgg cctgggcagc gttggcgacg
      961 ccgcgtggcc ggggaaggat ggcggatgag tcgatttcga tgggggctga cgcatgaccc
     1021 gaccctcgcg tgggccgggc cgttcccgga ctttcgttcc cgcagcggga gaggacgtta
     1081 cctcgcgggc cggaccctat ccgcccgccc cggcctgtca accggggttg ccgtgtccgt
     1141 cactctacct tcaggcgctt ggcgaagctg accgccggtc agcccgccgg ccccgcagcc
     1201 tcagttcagc agccccacct ccgtcattgc cgcccgcacc ttcgcgcgcg tcggctccat
     1261 gatcggcgcc agcggcaggc ggcagtgttc ggtgctcttg cccagcagcg aagcggcgaa
     1321 cttcaccggg gccgggctgg tctcggtgaa cagcgcgtcg tgcagcggca gcaggcggtt
     1381 ctggatctcc atcgcctgct gcatacggcc ctccatccag gccgtctgca tctcgctgca
     1441 caggcgtggc gcgacgttgg cggtgacgct gatacagccc acgccgccgg ccgcgttgaa
     1501 cgccagcgcg gtggcgtcct cacccgacaa ctggcagaaa tcggcaccgc actcccggcg
     1561 ctgatgcagc ggccgagcga gatcggcggt ggcgtccttc acgccgacga tgttcttgtg
     1621 cttcgccagc cgcgccatcg tctccacttt catgtcgatc acgctgcgcg gcgggatgtt
     1681 gtagatgatg atcggcaggt ccaccgcgtc ggcgatcgcc ttgaagtgca ggtacatgcc
     1741 ttcctgcgtc ggcttgttgt agtacggcgt cacgatcagg gctgcatccg cgccagcctc
     1801 cttggcatgg cgggtcagcc cgatggcctc ttccgtgctg ttcgatcccg cgccggcgat
     1861 caccggcacg cggcccttgg ccacctcgac gcagatctcc accacccgct tgtgctcgtc
     1921 atggctgagg gtcgggctct cgccggtggt gcccaccgga accaggccgt gcgtgccctc
     1981 gttgatctgc cactccacca gttcggcgta cgccttctca tccaccgacc cgtcagcacg
     2041 catcggggtg atcagagcga cgagcgatcc cttgaacatg gtcctctccg gggtggggct
     2101 aggcgaaggc gggaggctag gcgcgccagg ggcgtctcgc aaggccgcga acaaccctcg
     2161 atgggcattc cggcgcttcc ctgcaggcga tttacgtccg cctggtcagc agcagccgcg
     2221 ccgccaggct caccagcagc acaaagcccg tggtcaggaa cgccgccgca taggccagtt
     2281 cattcgctgc cgcgaagggc tcattgatga aggcccagat cgcataggtc agatagccga
     2341 tcggcgaatg ggtcaactgc ccgttccaca gatagctcga ccaaccggcg gtgtacagca
     2401 gcggagcggt ttcacccacc gaaatggcca gggccagcag cacgccggtc aacacgccgg
     2461 gcagcg
//
LOCUS       DASSZH010000298         3084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_245974, whole genome shotgun sequence.
ACCESSION   DASSZH010000298 DASSZH010000000
VERSION     DASSZH010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245974"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..670)
                     /locus_tag="VFN42_06415"
     CDS             complement(29..670)
                     /locus_tag="VFN42_06415"
                     /inference="COORDINATES: protein motif:HMM:NF021141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2459 domain-containing protein"
                     /protein_id="HET8996284.1"
                     /translation="MRRLPPVLVVALTFALAGCAAVIAPFPSGPATHAIGIAERSWHT
                     DLCVAAPTRPDDPLAPFAVGFVGARFLCFGFGERQYLLGGDHGVLEMLSALLPSQSAL
                     LMTVLSAPPDAAFGAENVVTLRVPAASEAAVRGFIRDSVRLDADGRPVRLQGGPYPGS
                     VFFAGTGTYDAVRTCNTWTADGLAAAGLSVGGPVLFAGQVMSQVRQIAARQGG"
     gene            complement(743..1963)
                     /locus_tag="VFN42_06420"
     CDS             complement(743..1963)
                     /locus_tag="VFN42_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HET8996285.1"
                     /translation="MSEIPPPTGPLRGLRVFDLTRVLAGPTCVQMLGDLGAEVIKIEK
                     PGSGDDTRGFVPPTLPGSTESAYFAGVNRNKFSVTLDIAQPEGQAIAQRLLDHCDILA
                     ENFKAGSLARYGLGWEQVRNSHPGLIYCSITGFGQTGPYASRPGYDSLVQAMGGVMSL
                     TGEPDGMPQKVGVPVADVFAGLYACIGILAALRHKERTGEGQSIDIGMLDTNVAWLSN
                     QGMNYLATGENPPRLGNQHPNIVPYQVFATADGHVVLSVGNDPTFRRFCEAFDLGHLL
                     DDPRFATTAARVQNRALVTETLAPVLRAHPTAWWVERLESLSIGGGPINRLSDVFADP
                     QVKARNMVVEMPHPTAEGGRVKVLANPVRLSATPPDYRIAPPLLGEHTEHVLGGLLGV
                     SSAEIADLRERGIV"
     gene            2068..>3084
                     /locus_tag="VFN42_06425"
     CDS             2068..>3084
                     /locus_tag="VFN42_06425"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET8996286.1"
                     /translation="MSGNRFLPAKFIPPRCRFRDGGPALNGTQLGIGQMKLKHALLAS
                     SFMLLPTVAMAQPISGLYVGAGAGANFPFAEPNKGFFAPGGAVLGVHDGAHFDTGWLG
                     VGSVGYGFGNGLRAEIEGDFYRNQLGSVSGASAANGFENKYGGFLNVLYDIDVAPWGI
                     PVIQPYVGIGAGYQWAHWGGVYAGLPPAQLQAGGTASGFAYQGIVGMAVPVPQMPNLA
                     LTVEYRVMDMPFDRKYNASIATPAGSFGGVVKMGQQIDQSVLVGLRYAFNVAPPPPPP
                     APAPAPVAAPAPAPSRTYLVFFDWDKADLTDRARQVISEAAQNSTRVQYTRLQVSGYA
                     DRTGS"
BASE COUNT          504 a         1046 c         1052 g          482 t
ORIGIN      
        1 tatgcgccaa cggcacgcag ccgccctgct agcccccctg ccgcgcggcg atctgccgca
       61 cttgtgacat cacctggccg gcgaacagca ccggcccgcc gaccgacagc ccggcagcgg
      121 ccagcccgtc ggcggtccag gtgttgcagg tccggaccgc gtcataggtg ccggtgccgg
      181 cgaaaaagac gctgcccgga tacgggccgc cctgcagccg caccggcctg ccgtccgcat
      241 ccaatctcac ggagtccctg atgaagccgc gcaccgccgc ctcgctcgcc gcgggcacgc
      301 gcagcgttac cacgttctcc gccccgaagg cggcgtccgg cggggcgctg agcacggtca
      361 tcagcagcgc cgactgactc ggcagcaggg cggagagcat ctccagcacg ccgtgatcgc
      421 cgccgagcag atactgccgc tcgccgaagc cgaagcagag gaaccgcgca cccacgaacc
      481 cgacggcaaa gggcgccagc ggatcatcgg gccgcgtcgg cgcggcgacg cacagatccg
      541 tgtgccagct tcgttcggcg atgccgatgg catgcgtggc cgggccggac ggaaacggcg
      601 cgatgacggc ggcgcagccg gcaagcgcaa aagtgagcgc aaccaccagc accggcggca
      661 accggcgcat ggcggcgcca gagcatgatc gcgcgaggcg gccacgtcgg agcgcgtgaa
      721 tcatgctctc aaacaaagag ctctagacga tcccgcgctc gcgcaggtcc gcgatctcgg
      781 cgctgctcac cccgagcaat ccgcccagca catgctcggt atgctcgccg agcagcgggg
      841 gcgcgatgcg gtagtcgggc ggggtggcgg acaggcgcac cgggttcgcc agcaccttca
      901 cgcggccgcc ctcggcggtc gggtgcggca tctccaccac catgttgcgc gccttcacct
      961 gcgggtcggc gaacacgtcg gacaggcggt tgatcggccc gccgccgatc gacaggctct
     1021 ccagccgttc cacccaccac gcggtcgggt gggcgcgcag caccggcgcc agcgtctccg
     1081 tcaccagcgc gcgattctgt acgcgcgcgg cagtggtggc gaagcgcggg tcatcgagca
     1141 ggtggccgag gtcgaacgcc tcgcagaaac ggcggaaggt tgggtcgttg ccgaccgaca
     1201 gcaccacatg cccgtccgcg gtcgcgaaca cctgatacgg cacgatgttc gggtgctggt
     1261 tgcccagccg cggcggattc tcgccggtcg cgagataatt catcccctgg ttggaaagcc
     1321 aggcgacgtt cgtatccagc atgccgatgt cgatagactg tccctcgccg gtccgttcct
     1381 tgtggcgcag cgcggccagg atgccgatgc aggcatagag cccggcgaac acatcggcga
     1441 ccggaacgcc cactttctgc ggcatgccgt ccggctcccc ggtcaggctc atcacgccgc
     1501 ccatcgcctg caccaggctg tcatagcccg gccgcgacgc atacggcccg gtctgcccga
     1561 atccggtgat ggagcagtag atcaggccgg gatggctgtt gcgcacctgc tcccacccca
     1621 gtccgtagcg ggcgaggctg ccggccttga agttctccgc caggatgtcg caatgatcga
     1681 gcaggcgttg cgcgatcgcc tgcccctccg gctgcgcgat atccagtgtc accgagaact
     1741 tgttgcggtt gacgccggcg aaatacgcgc tctcggtgct gccgggcagc gtcggcggca
     1801 cgaatccgcg ggtatcgtcg ccgctgccgg gtttctcgat cttgatcact tcggcgccaa
     1861 gatcgcccag catctgcacg caggtggggc cggcgagcac gcgggtgaga tcgaacacgc
     1921 gcaggccgcg cagcggaccg gtggggggcg ggatttcgct catgccgctt tctataggcg
     1981 ccggcgaagt ccagggaaac cgggggtcat tccagcgggt gttgcaccgt agcaacagtt
     2041 ccggggagac tgtcgcgccg gatcgtgttg tccgggaacc gcttccttcc ggctaagttc
     2101 atcccgccgc ggtgcaggtt ccgggatggg gggcctgccc taaacggaac tcagctaggg
     2161 atagggcaga tgaaactgaa acacgccttg ctggccagct cgttcatgct cctgccgacg
     2221 gttgcaatgg cacagccaat cagcgggctt tacgtcggtg ccggggccgg cgccaatttt
     2281 ccgtttgctg aacccaacaa gggctttttc gcgccagggg gcgctgtgct cggtgtgcat
     2341 gacggcgccc atttcgatac cggctggctt ggcgtgggca gcgtaggtta cggatttggg
     2401 aatggcctgc gcgctgaaat cgagggcgat ttctaccgca atcagctcgg cagcgtcagc
     2461 ggcgccagcg ccgccaacgg cttcgagaac aagtacggcg gcttcctgaa cgtgttgtac
     2521 gacatcgatg tggcgccgtg gggcatcccg gtgatccagc cgtatgtcgg catcggcgcc
     2581 ggttatcagt gggcgcattg gggcggcgtc tatgcgggtc tgccgccggc gcagctgcag
     2641 gcgggcggga cggccagtgg gttcgcctac cagggcatcg tcggcatggc ggtgccggtg
     2701 ccgcagatgc ccaatctcgc gttaacggtc gaatatcgcg taatggatat gccgttcgac
     2761 agaaaataca acgccagcat cgccacacct gcaggcagtt tcggtggtgt ggtgaagatg
     2821 ggccagcaga tcgaccagag cgtactggtc ggcctgcgct atgcgttcaa cgtcgcgccg
     2881 ccgccgccgc cgccggcccc tgcccccgcg ccggtcgcgg ccccggcccc ggcgccctcg
     2941 cgcacctatc tggtgttctt cgactgggac aaggcggacc tgaccgaccg cgccaggcag
     3001 gtgatctccg aggccgcgca gaattcgaca cgggtgcagt acacccggct gcaggtgtcg
     3061 ggctacgcgg accggaccgg ctcg
//
LOCUS       DASSZH010000299         7208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_859360, whole genome shotgun sequence.
ACCESSION   DASSZH010000299 DASSZH010000000
VERSION     DASSZH010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7208
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859360"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VFN42_06430"
     CDS             complement(<1..362)
                     /locus_tag="VFN42_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8996287.1"
                     /translation="MPHLKRTLRTLACMTLGAGLALAAGAAHAQKKPITIGFDIEETG
                     GLAPNGKAVLLAYKIWAEETNARGGLLGRPVKLLYYDDQSNPAMVPGIVTKLLDVDHV
                     DILLGENGTNLIAPAMPLV"
     gene            complement(431..1444)
                     /locus_tag="VFN42_06435"
     CDS             complement(431..1444)
                     /locus_tag="VFN42_06435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8996288.1"
                     /translation="MRYGLIIRGQYPQGDDMRLRLQEDLHAAKRAEELGYDLVAKGSH
                     YSSHPFQYIQQIPYLCQVAMVAPKLRLLPGVVLLPLHSPLHVAEELACLDVMSGGKLI
                     FGAGIGYREVEFRAFGTTLKQAGKRFEECLTAVKRLWSEEFVTMQASYFSLEGANCTT
                     MPLQKPMPPVWIGANANVGIRRAARMADAWFINPHNKLDTTAQQMEVYKRALDEAGKP
                     FPAELPMAREVFVARSREEAIRLARPSLEAKYKAYRDWGQDKVMPASDHFDLDFAELM
                     EDRFLFGSPAEVTEQILELKRRFGVTTLILGVHWVGMPNSLALETMQLLAEDVFPAVN
                     SAG"
     gene            complement(1544..2746)
                     /locus_tag="VFN42_06440"
     CDS             complement(1544..2746)
                     /locus_tag="VFN42_06440"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HET8996289.1"
                     /translation="MRIARIEDLHCNAGWRDFSFLKITSDDGTVGWSEFMENFGAEGL
                     GTVIQRLGEHIIGMDPRAVEKVTAFLHGLTRQSPYGINQQAIAAIENALVDLKAKSLG
                     IPVYELLGGPVRDRLQLYWSHCGTWRVSFAEQIKQWTGFDPIRSLADVERAGAEVARR
                     GFRGLKTNIFRFDTDPPSVHMPGFNGPGWPALNIDAALVDGLVAQMGAFRAGAGPGVG
                     LHLDLNFNFKTEGYIRLAQALEPFDLVWLEIDSYDPAALATIRRSARTRIASCESLYG
                     RRQFRPYFEQQSVDVAIIDVAWNGILESMKIAAMADAYEINVAPHNFNGHIGSLMSAH
                     LCAAIPNFRVMEIDIEDVPWKDDLVSVPPRIEQGELLIPTGAGWGAEVNEAVIRAHPP
                     RRPVKWQT"
     gene            2881..4119
                     /locus_tag="VFN42_06445"
     CDS             2881..4119
                     /locus_tag="VFN42_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8996290.1"
                     /translation="MESTCSRHGLNWLNFFVAAVQTGFGPFVSVYLTQQHWTQTDIGL
                     VLSLGAGVALFAQLPAGALVDALYSRRHLTALALAVVGVSALMLAARPSWATVRDSQI
                     LHAVGSCVLTPAIAAITLSLCGHAGYSERLGVNARYASLGNAIGAALLGACSYYLDER
                     AVFLVTAALTLPAILATYAVRPRDRIVSMHEHPATWHPQKRKQHQHRMWHIAGERGLH
                     VFAACAVLFGLANAAMLPLALNLLSQRIPESGFIITAVILLPQAISAAGSPWVGRLAQ
                     RIGRKPVLLAGFLAVPLRGVLFAVLFLDRPGALAVVLLQGLDGISAMVFGLMLPLVAA
                     DVTQKHGFMNTAIGWFALAVGLGAVFSTTLGGWMADQLGAPAAFLGLSLAGALGVLLL
                     AFGMPETRPLSAATQPAAVA"
     gene            4184..5299
                     /locus_tag="VFN42_06450"
     CDS             4184..5299
                     /locus_tag="VFN42_06450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HET8996291.1"
                     /translation="MSDTASHLHEPGGDFQCLHEFIPAARAKLSDHIWGYLVGATETE
                     TTMRRNRLALDSIALRPRVCRDVSQIDTSTTLFGRKLRMPVMLAPVGSLESFHPGGAA
                     TAGRAASEFGIPIIVSSVTKPGLEEAAAAAPGPKVFQLYVRGDNAWVDDVVRRAAGAG
                     YDAFAITVDTAAYSRRERDIAGRFVKPWRSAATGHEFQAGFSWDNVRHFKDTHTIPLI
                     LKGIATAEDAALACEYGVDCVYVSNHGGRQLDHGRGSMDVLPEVLAAVRGRAKVVVDG
                     SFCRGTDIVKAIALGADAIAMGRMYCYALAAGAEAGVLKMLELLEHELGVAMALSGAR
                     KLGELNPSFLHMGAPSVAAPHVHSAFPLLFTTPEHRG"
     gene            complement(5353..7146)
                     /gene="aspS"
                     /locus_tag="VFN42_06455"
     CDS             complement(5353..7146)
                     /gene="aspS"
                     /locus_tag="VFN42_06455"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET8996292.1"
                     /translation="MHAYRTHTCGALRAADAGKPARLSGWVHSKRDHGGLLFIDLRDH
                     YGLTQCVFPAGSPAFAAADAVRPESVITVTGEVVPRESGTVNPKLPTGEIELRVNALV
                     VQSAAEVLPMQVAGEEKYPEDIRLKYRFVDLRRDKIHRNIMLRAGVISSIRRRMIEAG
                     FTEFQTPILTASSPEGARDFLVPARLHPGKFYALPQAPQQFKQLIMVAGFDRYFQIAP
                     CFRDEASRADRSPGEFYQLDFEMSYVTQEDVFQTIEPVIAGVFEEFAEGRAVTQGAFP
                     RIPYDTAMLQFGSDKPDLRNPLRITDVTAQFAGSGFGLFARIAASGGVVRAIPAPGAA
                     SQPRSFFDKLNEWARAEGAGGLGYIVFDAEGPKGPIARNLEADRAEAIRAACGLQAGD
                     AVFFAAGTKDAAPKFSGVVRTRIADELDLSEKNAFRFCWITDFPMYELNEETGQIDFS
                     HNPFSMPQGGMEALNSQDPMTIKAFQYDIVCNGVELSSGAIRNHRPDIMIRAFEIAGY
                     SADEVERRFGGMLNAFRYGAPPHGGSAPGIDRIVMLLAEEPNIREVIVFPLSQGGEDL
                     MMGAPAEVSAARLKELHLRIELPPKAKTQAG"
BASE COUNT         1083 a         2427 c         2448 g         1250 t
ORIGIN      
        1 accagtggca tggccggcgc tatcaggttg gtgccgttct cgcccagcag tatgtcaaca
       61 tggtccacat ccagcagctt ggtgacgatg ccgggcacca tcgccggatt ggactggtcg
      121 tcgtaataca gcagcttcac cggacggccg agcagcccgc cgcgggcgtt ggtttcctcg
      181 gcccagatct tgtaggcgag cagcacggcc ttgccattgg gcgccaggcc gccggtttcc
      241 tcgatatcga agccgatagt gatcggcttc ttctgggcgt gcgcggcgcc ggcggcaagc
      301 gccaagccgg cgcccagggt catgcaggcg agcgtgcgga gtgtccgctt gagatgcggc
      361 atcgtgggtt tcctcccggg ttttgtcgtc tgccgcaacg ttgggccagg gccgcggcgg
      421 agtaaaccgg ctacccggcg ctgttcaccg ccgggaacac atcctcggcc agaagctgca
      481 tcgtctccag cgccaggctg ttcggcatgc cgacccagtg gacgcccagg atcagcgtgg
      541 tgacgccgaa gcggcgcttc agttccagga tctgttccgt cacttccgcc ggcgagccga
      601 acaggaagcg gtcttccatc agttcggcaa aatccaggtc gaagtgatcg ctcgccggca
      661 tcaccttgtc ctggccccag tcgcgatacg ccttgtattt cgcttccagt gacgggcgtg
      721 ccagccggat cgcttcctcg cgcgagcgcg cgacgaacac ctcgcgtgcc atcggcagtt
      781 ccgccgggaa cggcttgccg gcttcatcca gcgcgcgctt gtagacctcc atctgctgtg
      841 ccgtggtgtc cagcttgttg tgcggattga tgaaccaggc atccgccatg cgggcggcgc
      901 ggcggatgcc gacattggca ttggcgccga tccataccgg cggcattggc ttctgcagcg
      961 gcatcgtcgt gcagttggcg ccttccagcg agaagtagga tgcctgcatg gtgacgaact
     1021 cttccgacca cagccgtttc accgcggtca ggcattcctc gaagcgcttg ccggcctgtt
     1081 tcagcgtggt gccgaaggcg cggaactcga cctcgcgata gccgatgccg gcgccgaaga
     1141 tcagcttgcc gccgctcatc acgtcgaggc aggccagctc ctccgccaca tgcagcgggc
     1201 tgtgcagcgg cagcagcacc acgccgggca gcaggcgcag cttcggggcc accatcgcca
     1261 cctggcagag atagggaatc tgctggatgt actggaacgg atgcgagctg tagtgggagc
     1321 ctttcgccac caggtcgtaa cccagttcct cggcacgttt cgcggcatgc aggtcttcct
     1381 gcaggcgcag ccgcatgtcg tcgccttgcg gatattggcc gcggatgatc agaccgtagc
     1441 gcatgctggc tcctccggtt ggttggggca ggctaaccgg cggcctcccc cgtgtcatgg
     1501 cctcaccgct taagcacgat cagccgcgac gtgtggttgg cggtcaggtt tgccacttca
     1561 ccggccgccg cggcggatgc gcgcggatca ccgcttcgtt cacctcggcg ccccagcccg
     1621 cgccggtcgg gatcagcaac tccccttgct cgatgcgggg cggcacgctt accaggtcgt
     1681 ccttccaggg cacgtcctca atgtcgatct ccatcacccg gaagttcgga atcgccgcgc
     1741 agagatgcgc gctcatcaga ctgccaatgt ggccgttgaa gttgtggggc gccacgttga
     1801 tctcataggc gtccgccatc gcggcgatct tcatcgattc caggatgccg ttccaggcca
     1861 cgtcgatgat cgccacgtcc accgattgct gctcgaaata gggccggaat tgccggcggc
     1921 catacagcga ctcgcaggat gcgatgcggg tgcgtgcgct acgccggatg gtggccagcg
     1981 ccgccgggtc gtagctgtcg atctccagcc agaccaggtc gaacggctcc agcgcctggg
     2041 ccaggcggat gtagccctcg gtcttgaagt tgaagttcag atccagatgc agcccgaccc
     2101 ctggtcccgc cccggcgcgg aacgcgccca tctgtgccac cagcccgtcc accagcgcgg
     2161 cgtcgatatt cagcgccggc cagccggggc cgttgaaccc gggcatgtgc acactcggcg
     2221 ggtcggtgtc gaagcggaag atgttggtct tcagaccccg gaagccgcgg cgtgccacct
     2281 cggcaccggc gcgctcgaca tcggccagag aacggatcgg atcgaacccc gtccactgct
     2341 tgatctgctc ggcaaagctc acccgccagg tgccgcaatg cgaccagtaa agctgcagcc
     2401 ggtcgcgcac cgggccgccc agcagttcat aaaccgggat gccgagcgac ttggctttca
     2461 gatcgaccaa cgcgttctcg atcgcggcga tcgcctgctg gttgatcccg tagggcgact
     2521 gccgggtcag cccgtgcagg aaggcagtga ccttctccac cgcgcgcgga tccatgccga
     2581 tgatgtgctc gcccaggcgc tggatcaccg tgccgaggcc ttcggcgccg aagttctcca
     2641 tgaactccga ccagccgacc gtgccgtcgt cgctggtgat cttcaggaag gagaagtcgc
     2701 gccagccggc gttgcagtgc aggtcctcga tcctggcgat gcgcatggcg ttccctccgc
     2761 gttgcagggg cagcctaggc cgctccggcg ttccgaggaa cccggcgccg gttccgtttg
     2821 ttcggcgccg ccagtcgggc tattccacga agcttccgcg gctggcaatc gacacccaca
     2881 gtggaatcca cctgttcccg gcacggcctg aactggctca acttcttcgt cgccgcggtg
     2941 cagaccgggt tcggcccctt cgtgtcggtc tacctgacgc agcagcactg gacgcagacc
     3001 gatatcggcc tggtgctgag cctgggagcc ggtgtcgcgc tgttcgcgca attgccggcc
     3061 ggggcgctgg tggatgcgct ctacagtcgg cgccacctga ccgcgcttgc tctggccgtg
     3121 gtgggcgtca gcgccctgat gctggcggcg cggccgagtt gggcgaccgt ccgcgattcg
     3181 cagatcctgc atgcggtggg aagctgcgtg ctgacgccgg cgatcgccgc catcacgctg
     3241 tcgctgtgcg ggcatgctgg ctacagcgag cggctggggg tgaatgcgcg ctatgcgtcg
     3301 ctgggcaacg cgatcggggc ggcgctgctg ggggcctgct cctattacct ggatgagcgc
     3361 gcggtattcc tggtcaccgc ggcgctgaca ctgccggcga tcctggcgac ctatgccgtg
     3421 cggccgcgcg accggatcgt ctcgatgcac gagcatccgg ccacctggca tccgcagaag
     3481 cgcaagcagc accagcaccg catgtggcac atcgcgggcg agcgcgggct gcacgtgttc
     3541 gccgcctgcg cggtgctgtt cggcctggcc aatgcggcga tgctgccgct ggcactgaac
     3601 ctgctgtcgc agcgcatccc cgaatcgggc ttcatcatca ccgcggtgat cctgctgccg
     3661 caggcgatca gcgccgccgg ttcgccctgg gtgggccggc tggcgcagcg catcgggcgg
     3721 aagcccgtgc tgctggccgg gtttctggcg gtaccgctgc gcggcgtgct gttcgcggtg
     3781 ttgttcctgg atcgcccggg cgccctcgcg gtggtcctgc tgcaggggct ggacggcatc
     3841 agcgccatgg tgttcggcct gatgctgccg ctggtcgccg ctgacgtcac gcagaagcac
     3901 ggcttcatga acacggcgat cggctggttc gcgctggccg tcggccttgg cgccgtattc
     3961 agcaccacgc tggggggctg gatggcggac cagcttggcg caccggcggc tttccttggc
     4021 ctgtcgctcg ccggcgcgct gggtgtgctg ctgctggcgt tcggcatgcc ggaaacccgg
     4081 cccctttcag cggccacgca accagcggcg gtggcctgac cccttgcggt tgtggcgcgg
     4141 ctgacgcctg atacagcggc gaattatcgg gagagtccgc accatgtccg acaccgcgtc
     4201 gcacctgcac gagcccggcg gcgatttcca gtgcctgcac gaattcattc cggccgcgcg
     4261 tgccaagctc agcgaccata tctggggcta cctggtcggc gccaccgaga ccgaaacgac
     4321 catgcggcgc aaccggctgg cgcttgattc catcgcgctg cgcccgcggg tctgccgcga
     4381 cgtgtcgcag atcgacacca gcaccacgct gttcggtcgc aagctgcgca tgccggtgat
     4441 gctggcgccg gtcggttcgc tggaatcgtt ccatccgggc ggcgcggcca ccgccggccg
     4501 cgccgcatcg gagttcggca tcccgatcat cgtcagctcg gtgaccaagc cgggcctgga
     4561 ggaagccgcc gccgcggcgc ccggcccgaa ggtgttccag ctttatgtcc gcggcgacaa
     4621 tgcctgggtg gatgacgtgg tgcggcgcgc ggccggcgcc ggctacgacg ccttcgccat
     4681 caccgtggac accgccgcgt acagccggcg cgagcgcgac atagcgggcc gcttcgtcaa
     4741 gccgtggcgc agcgcggcga ccgggcacga attccaggcc gggttctcct gggacaatgt
     4801 caggcacttc aaggacacgc acacgatccc gctgatcctg aagggcatcg ccaccgcgga
     4861 agatgccgcg ctggcctgcg aatacggggt ggactgcgtg tacgtgtcca accatggcgg
     4921 gcggcagctt gatcacggcc gcggttcgat ggacgtgctg ccggaagtgc tggctgcggt
     4981 gcgcggccgc gccaaggtgg tggtggacgg cagcttctgc cgcggcaccg acatcgtcaa
     5041 agccatcgcg ctcggcgccg acgcgatcgc gatggggcgc atgtactgct acgcgctggc
     5101 cgccggagcc gaggccggcg tgctgaagat gctggagctg ctggagcacg agctcggcgt
     5161 ggcgatggcc ctgagcggcg cgcgcaagct gggcgagctg aacccgtcct tcctgcacat
     5221 gggggcgccg agcgtcgccg caccgcacgt gcacagcgcg ttcccgctgc tgttcaccac
     5281 cccggagcat cggggatagc agcggcgtgg cccggccatg gttccaggac catggcccgc
     5341 cacgcccttc ggctaacctg cctgcgtctt cgccttcggc ggcagctcga tgcgcagatg
     5401 cagttccttc agccgggccg cggagacttc cgcgggagcg cccatcatca ggtcctcgcc
     5461 gccctggctg agcgggaaca cgatcacctc gcggatgttc ggctcctcgg ccagcagcat
     5521 gacgatgcgg tcgatgcccg gcgcggaacc gccgtgcggc ggggcgccgt agcggaacgc
     5581 gttcagcatg ccgccgaacc gccgctcgac ttcatccgcg ctgtagccgg cgatctcaaa
     5641 ggcgcggatc atgatatccg ggcggtggtt gcggatcgcg ccggatgaca gctccacgcc
     5701 gttgcagacg atgtcgtact ggaacgcctt gatggtcatc gggtcctgcg aattcagcgc
     5761 ctccatcccg ccctgcggca tgctgaacgg gttgtggctg aagtcgatct gcccggtttc
     5821 ctcgttcagc tcgtacatcg ggaagtcggt gatccaacag aagcggaacg cgttcttctc
     5881 gctcaggtcc agctcatccg cgatgcgggt gcgcacgacg ccggagaatt tcggcgccgc
     5941 gtccttggtg ccggcggcaa agaacaccgc gtcgccggct tgcagcccgc aggccgcacg
     6001 gatcgcctcg gcgcggtcgg cttccaggtt gcgcgcgatc ggtcccttcg gtccttcggc
     6061 atcgaacacg atgtagccca gaccgccggc accttccgcg cgcgcccatt cgttcagctt
     6121 gtcgaagaag ctgcgtggct gcgacgccgc gcccggcgcg ggaatggcgc gcaccacgcc
     6181 gccgctcgcc gcgatgcgcg cgaacaggcc gaagcccgag ccggcgaact gcgcggtcac
     6241 gtcggtgatg cgcagcggat tgcgcaaatc cggcttgtcg ctgccgaatt gcagcatcgc
     6301 cgtgtcatag ggaatgcgcg ggaacgcgcc ctgggtcacc gcgcggccct cggcgaattc
     6361 ctcgaacacc ccggcgatca ccggctcgat ggtctggaac acgtcttcct gggtgacgta
     6421 gctcatttcg aaatcgagct ggtagaactc gcccggcgag cggtccgcgc ggctggcctc
     6481 atcacggaaa cacggcgcga tctggaagta tcggtcgaat ccggcgacca tgatgagctg
     6541 cttgaactgc tgcggcgcct gcggcagcgc gtagaacttc ccggggtgca gccgagcggg
     6601 gaccaggaag tcgcgcgcgc cttccgggct ggaggcggtg aggatcggcg tctggaactc
     6661 ggtgaagccc gcttcgatca tgcggcggcg gatggaggag atcaccccgg cgcgcagcat
     6721 gatgttgcgg tggatcttgt cccgccgcag atcgacgaag cggtatttca gccggatatc
     6781 ctccgggtac ttctcctcgc cggccacctg catcggcagc acctcggccg ccgactgcac
     6841 caccagcgcg ttcacccgca actcgatttc gccggtgggc agcttggggt tcaccgtgcc
     6901 ggactcgcgc ggcaccacct cgccggtgac ggtgatcacg ctttccggcc gcaccgcatc
     6961 ggccgcggcg aaggcggggg aaccggcggg gaacacgcat tgcgtcaggc cgtaatggtc
     7021 acgcagatcg atgaacagca gcccgccatg gtcgcgcttg gagtggaccc agccggacag
     7081 gcgggccggc ttgccggcat cggcggcgcg gagcgcgccg caggtatggg tgcggtaggc
     7141 gtgcatcatc gggttcccag gcagagggcg ggcagacaaa ccccgggctt tccggggagt
     7201 caaggcgg
//
LOCUS       DASSZH010000300         7247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_927459, whole genome shotgun sequence.
ACCESSION   DASSZH010000300 DASSZH010000000
VERSION     DASSZH010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7247
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927459"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1514)
                     /locus_tag="VFN42_06460"
     CDS             complement(<1..1514)
                     /locus_tag="VFN42_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HET8996293.1"
                     /translation="MIIELGHFALILALCVALVQSIVPLIGAARGNTAWMATGRSCAI
                     AVFLLTAISMLALMHAYVTNDFSVLNVVENSHTDKPLLYQITGVWGNHEGSMLLWVFM
                     LSLCAAAVAVFSNNLPPELRARVLAVQGMIGVGFLLFILLTSNPFLRIFPAPPNGQGL
                     NPVLQDPGLAFHPPFLYLGYVGFSVAFAFAVAALIEGRVDAAWARWVRPWTLASWCAL
                     TIGIAMGSWWSYYTLGWGGWWFWDPVENASLMPWLVGTALIHSSIVVEKRDTLKSWTI
                     LLAIITFSLSLVGTFLVRSGVLSSVHAFATDPKRGMFILLLLLVAIGGSLTLYAVRAP
                     ALKGGGLFAPISREGALVLNNLLLSIGCATVFLGTLYPLFLDAVGGPKLSVGFPFFNR
                     TFAPLMVPMLIAVGIGPLLAWKRGDILGALQRIWLAYIMCGIALLIGFYVTHGGPVLA
                     VLGLGLAAWLATAVVVEWAERVKLFRAPLHDSWKRAIHLPRATYGMSIAHFGLAV"
     gene            complement(1665..2882)
                     /gene="hemA"
                     /locus_tag="VFN42_06465"
     CDS             complement(1665..2882)
                     /gene="hemA"
                     /locus_tag="VFN42_06465"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HET8996294.1"
                     /translation="MSLFHAYCRQSLDNIRAQGRYREFTALEKQAERFPYYLRPDGSE
                     VLVWSSNDYIGMGGHPVVVRAACEAAERMGAGAGGTRNISGTSPVHDALEAELADLHG
                     KQAALVFTSGFVSNQAALSTILNSRPAGPDALWHVFSDAKNHASMIAGIKGARAVVHI
                     FRHNDLEHLEALLQEAPPAAPKMVAFESVYSMDADIAPIGAICDLATRYGALTYLDEV
                     HAVGMYGPNGGGVSERDGVAHRVDIIEGTLAKAFGCHGGYITGDAEVVDFIRSVAPGF
                     IFTTSMPPMVAAAALASVRHVRKDHMRRARLFERADALKRRFDAAGLPRIESASHIVP
                     LHIGEAALCKEVSRRLLDEFGMYATPINYPTVPRGEERLRFTPTPLHTDAMMTRLVDA
                     LLQILRPAQRAAA"
     gene            3176..4579
                     /locus_tag="VFN42_06470"
     CDS             3176..4579
                     /locus_tag="VFN42_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET8996295.1"
                     /translation="MLIPLKLVRDQPLQQQIFDQLRGLIQSGRLAPEMRMPSTRMVAD
                     QFAVSRITVLLVYERLIAEGYLETIPAKGTFVSHPPAELSGRTSGCPMHEDREASPLP
                     CCGGIGLTQVGRPDPGLFPLARWRALLRGAVDRLGNASPAEHAAGAVRLRQAIAGWLS
                     TSRGLAVAPDQIVLVSGRQQALHIASHLLLRTGARAVLEDPFDERTACTYSEAGADLV
                     CVPVDSRGLRPELLPGGSAALLHVTPEHQRPTGAPLGSERRAAVLDWAGRSGAIIVEE
                     DCDGEFRYGGMEAAPLMSLDQHERVLLIGGFAVALGPWVTLGYMAVPPSLVKPAREAR
                     RLIDDSAEALESSALAEFLESGAYARHVHRLRKIYLGRRDSLVTALRRHFGPVETWGA
                     GAGLHLTWQLPPELGTASALAERARQAGLEAEAVGPDDCCLLLGFGMLTESLLEGGVA
                     RLAVDANRRRPAARIAG"
     gene            4738..5139
                     /locus_tag="VFN42_06475"
     CDS             4738..5139
                     /locus_tag="VFN42_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996296.1"
                     /translation="MRLRSRLHYVIAVSAALFAMTGAGTSFAAETAKPPSFKQDVLPI
                     LQEHCAQCHAPGQIGYQAIGLDLTTYRGVMAGSRHGPTVIPYQPQIGSMMKVLDWKQD
                     YYVHMPAMSHQLPEADMNVIRDWIAAGAKDN"
     gene            5453..6673
                     /locus_tag="VFN42_06480"
     CDS             5453..6673
                     /locus_tag="VFN42_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET8996297.1"
                     /translation="MSARWARTASLMAAGALAALAWGAPAAHAQRHAPQAHKIDLLRP
                     LYANSMDIAEGKSVAQHFCADCHGPAGISTIPGVPDLAGQRAAYLYSEMRAYLSGARG
                     DDTMNGAITYLSADAMSNVAAYYGSLPPAAPAPAAKKGELGAVEAGKAAAATCGGCHG
                     ATGITATAGVPSLVGQEPKYLVTALGEYKDGSRKNDTMKAMAAPLDSVTMSNIALFYA
                     LEKPAKAPTPAPGDAAAGQTASAPCAACHGANGISGNPATPSLAGQDAQYLADALHGY
                     KSGARENTTMKALAASLDDATIKNLAAYYAGLQPQQPNVRKPLSTAEWVERCDRCHGP
                     GGNSADPLMPSLASQRAAYLEKVLNAYRAGKRNSPVMSAMMDGMSENDVRNLAQFYAS
                     QKAHAVVYVPVPAR"
     gene            6714..7019
                     /locus_tag="VFN42_06485"
     CDS             6714..7019
                     /locus_tag="VFN42_06485"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET8996298.1"
                     /translation="MNRILLTALVAAALGFAGAAHAAGDAAAGKAKAASCAMCHGPNG
                     EGNKMGPKLTGIAPAAFIKAMDDYKSGARANPMMKSAATPLSADDIANLAAYYASLK"
     gene            complement(7001..7168)
                     /locus_tag="VFN42_06490"
     CDS             complement(7001..7168)
                     /locus_tag="VFN42_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996299.1"
                     /translation="MRFISKLLSEVLLELTAVRRVDAVSGRGGLVVVCGGCGIATRSR
                     PLPEGGYFSDA"
BASE COUNT         1267 a         2523 c         2420 g         1037 t
ORIGIN      
        1 acggccaggc cgaaatgcgc gatgctcatg ccgtaggtgg cgcgcggcag gtggatcgcc
       61 cgcttccagc tgtcatgcag cggcgcccgg aacagcttca cccgctcggc ccactccacc
      121 accaccgcgg tggccagcca ggccgccagg ccgagaccca gcaccgccag caccggcccg
      181 ccatgcgtca cgtagaagcc gatcagcagg gcgatgccgc acatgatgta ggccagccag
      241 atgcgctgca gcgcgcccag gatgtcacca cgcttccagg ccagcagcgg gccgatgccc
      301 accgcgatca gcatcggcac catcagcggc gcgaaggtgc ggttgaagaa cggaaagccc
      361 accgacagtt tcggcccgcc caccgcatcc aggaacagcg gatacagcgt ccccaggaac
      421 accgtggcgc agccgatcga cagcagcagg ttgttcagca ccagcgcgcc ttcgcgggaa
      481 atcggcgcga acagtccacc gcccttcagc gccggcgcgc ggacggcata cagcgtcagc
      541 gagccgccga tcgccaccag cagcagcagc aggatgaaca tcccgcgctt cgggtcggtg
      601 gcgaaggcgt gcaccgagct cagcacgccg gagcgcacca ggaaggtgcc gaccagcgag
      661 agcgagaagg tgatgatcgc cagcaggatg gtccaggact tcagcgtgtc gcgcttctcc
      721 accacgatgg aggagtggat cagcgcggtg ccgaccagcc acggcatcag cgaggcgttc
      781 tcgaccgggt cccagaacca ccagccgccc cagcccagcg tgtagtacga ccaccaggag
      841 cccatggcga tgccgatggt cagcgcgcac caggacgcca gggtccaggg gcgcacccag
      901 cgtgcccagg cggcatcgac acggccctcg atcagcgcgg ccacggcgaa ggcgaaggcg
      961 accgagaagc cgacatagcc caggtagagg aagggcggat ggaaggccag gccgggatcc
     1021 tgcagcaccg ggttcagccc ctggccgttc ggcggcgccg ggaaaatccg caggaacggg
     1081 ttggaggtca gcaggatgaa cagcaggaag cccacgccga tcatcccctg caccgccagc
     1141 acgcgggcgc gcagctcggg gggcaggttg ttgctgaaca ccgccaccgc ggcggcgcac
     1201 agcgacaaca tgaacaccca caggagcatg gaaccctcgt ggttccccca cacgccggtg
     1261 atctggtaca gcagcggctt gtcggtgtgg ctgttctcga ccacgttcag cacgctgaag
     1321 tcgttggtca cataggcatg catcagcgcc agcatgctga tcgcggtcag caggaacacg
     1381 gcgatggcgc aggaccggcc ggtggccatc caggcggtgt tgccgcgcgc cgcaccgatc
     1441 agcggcacga tcgactgcac cagcgcgacg cagagcgcca ggatcagggc gaagtggccg
     1501 agttctatga tcatctacaa cattcctccc cctccccttg cgggaggggg ttggggggag
     1561 ggggcggttg gaagttcccg gggactgcaa cgacaggccc cgccccccca cccccccccc
     1621 gcgaggggag ggggaggagt gaaccctccc ccggtttggc atgctcaggc ggctgcgcgc
     1681 tgcgcggggc gcaggatctg cagcagcgcg tccaccagcc gcgtcatcat cgcatcggta
     1741 tgcagcggcg tcggggtgaa gcgcaggcgt tcctcgccac gcggcacggt ggggtaattg
     1801 atcggcgtgg catacatgcc gaattcgtcc agcaggcgcc ggctgacttc cttgcacagc
     1861 gcggcctcgc cgatatgcag cggcacgata tggctggccg actcgatccg cggcaggccg
     1921 gcggcgtcga accggcgctt cagcgcatcc gcccgctcga acagccgggc gcggcgcatg
     1981 tggtccttac gcacgtgccg cacgctggcc agcgccgcag cggcgaccat cggcggcatc
     2041 gacgtggtga agatgaagcc cggcgccacc gagcggatga agtccaccac ctcggcatcg
     2101 cccgtgatgt agccgccgtg gcagccgaag gccttggcca gcgtgccctc gatgatatcg
     2161 acccgatgcg ccacgccgtc gcgctcggac acgccgccgc cattcgggcc gtacatgccc
     2221 accgcgtgga cctcgtccag ataggtcagc gcgccgtagc gggtcgccag gtcgcagatg
     2281 gcgccgatcg gggcgatatc cgcatccatc gaatagaccg actcgaaggc caccatcttc
     2341 ggcgcggcgg gcggggcctc ctgcagcaac gcttccaggt gctccaggtc gttgtggcgg
     2401 aagatgtgca ccacggcgcg ggcgcccttg atgccggcaa tcatcgacgc gtggttcttg
     2461 gcgtcggaga acacgtgcca cagcgcatcc ggccccgccg gacggctgtt caggatggtc
     2521 gacagcgccg cctggttgga gacgaagccc gaggtgaaca ccagggccgc ctgtttcccg
     2581 tgcagatcgg cgagttccgc ctccagcgcg tcgtgcaccg ggctggtgcc gctgatattg
     2641 cgggtgccgc cggcgccggc gcccatgcgc tccgccgcct cacaggccgc gcggaccacg
     2701 accggatggc cgcccatgcc gatatagtcg ttcgacgacc agaccagcac ttcgctgccg
     2761 tccggccgca gatagtacgg gaagcgttct gcctgcttct ccagggcggt gaactcacgg
     2821 tagcgccctt gggcccggat attgtccagt gattggcggc agtaagcgtg gaacaggctc
     2881 attcgggcgg cacccctttt gcgtgtcagc acaggcaggc gatggctttc cggccacgat
     2941 tcgaccggtc aaatcctagg cgtgaggtca aactaccggt ctttgccggc atctcattga
     3001 catgaatcaa cgtcgcggtc cgcacgcatc agtaggtttc agcccgtcat cctagcggcc
     3061 aggagtcggg gactgaagtg gcataccgga atttcatacc ctccgcgtcg aacccgcacc
     3121 ggcattccgt ctttcgcgca ggaaacgccg caggctcgca ggcagggacc gagccatgct
     3181 gatcccgctc aagctggtgc gcgaccagcc gttgcagcag cagattttcg atcaactccg
     3241 cggcctgatc caaagcggcc ggctggcgcc ggaaatgcgc atgccgtcca cccgcatggt
     3301 ggccgaccag tttgccgtct cccgtatcac cgtgctgctg gtttacgagc ggctgatcgc
     3361 ggaaggctat ctggagacca tcccggccaa gggcaccttc gtcagccatc cgccggcgga
     3421 actgtccggc cggacctccg gctgcccgat gcacgaggat cgcgaagctt cccccctgcc
     3481 ctgctgcggc ggcatcggcc tgacccaggt cgggcggccc gaccccgggc tgtttcccct
     3541 ggcccgctgg cgcgcattgc tgcgcggtgc ggtggaccgg ctgggcaacg cctcgcctgc
     3601 cgaacacgcc gccggcgccg tccggctgcg ccaggccatc gccggctggc tgtccacctc
     3661 gcgtggcctc gccgtggcgc cggaccagat cgtcctggtc agcggccggc agcaggcgct
     3721 gcacatcgcc agccacctgc tgctgcgcac cggtgcccgt gcggtgctgg aggatccctt
     3781 cgacgagcgc acggcctgca cctattccga ggcgggcgcc gatctggtct gcgtaccggt
     3841 ggattcgcgc ggcctgcgcc cggaactgct gcccggcggt tccgccgcgc tgctgcacgt
     3901 caccccggaa caccagcgtc cgaccggtgc gccgctgggc agtgagcgac gcgccgcggt
     3961 gctggactgg gcgggccgca gcggcgcgat cattgtcgag gaggattgcg acggcgagtt
     4021 ccgctacggc ggcatggaag ccgcgccgct gatgagcctg gatcagcacg aacgcgtgct
     4081 gctgatcggc ggcttcgccg tggcgctggg gccgtgggtg acactgggct acatggcggt
     4141 gccgccatcg ctggtgaaac cggcccgtga agcacgccgc ctgatcgacg attccgcgga
     4201 ggccctggaa agctcggctc tggccgagtt cctggaaagc ggcgcctatg cccgccacgt
     4261 ccaccggttg cgcaagatct acctgggacg gcgggattcg ctggtcaccg cgctgcgccg
     4321 acacttcggc ccggtcgaaa catggggtgc cggcgccgga ctccacctga cctggcaact
     4381 tcccccggaa cttggcaccg cgtccgcgct ggccgaacgg gcgcggcagg ccggcctgga
     4441 ggccgaggcc gtcggaccgg acgattgctg cctgctgctg gggttcggca tgctgaccga
     4501 gtcgctgctg gagggcggcg tcgcccgcct ggcggtggac gccaaccggc ggcgcccggc
     4561 cgcccggatc gcgggctgag gttcgacacg gtgcataccc aagacgccgt ctcctcgtcc
     4621 ggcgtcacag caacgacatc ggctgctacg gcattgatct gcttgactca gatcaatgcc
     4681 agaattttca aatatcttat ggctatcccc gtcatgcaac gcgagaggag gcaggtcatg
     4741 cgcttgcgtt cgaggcttca ttacgtgatc gccgtatcgg ctgcgctctt cgccatgacc
     4801 ggcgcaggga ccagcttcgc agcggagacc gcgaaaccgc ccagcttcaa acaggacgtg
     4861 ctgccgatcc tgcaggagca ctgcgcccag tgccacgcgc ccggacagat cggctatcag
     4921 gcgatcggcc tggacctgac cacctatcgg ggcgtgatgg ccggatcgcg tcacggtccg
     4981 acggtgatcc cctaccagcc ccagatcggc tcgatgatga aggtgctgga ctggaagcag
     5041 gactactacg tccacatgcc ggcgatgagc catcagttgc cggaggcgga catgaacgtg
     5101 atccgggact ggatcgcggc gggcgcgaag gacaactgat ccgatcgccg cacatcggga
     5161 cctggtatca ttaatattct gtatcctgga actaggtcct gttccccagt ccggcgatca
     5221 tgcgcgacgg tcggtcctca gcgccgggtc aggtgcctgg cacatgcagc cgaccgcccg
     5281 cgcggggact gggatcacgg agcacactga cagcatgccc acgcacagca ccccggtttc
     5341 cacgggcggg tgcctgagcg gtttccgcac gccaggcatc ctgcgcacag gccagagttt
     5401 cgggggtatc atgaccaggt tgacttcgac cgcccggcgc gccgagccgc cagtgagtgc
     5461 gcggtgggct cgcacggcct cgctgatggc ggcaggcgcg cttgccgccc tggcatgggg
     5521 cgcaccggct gcgcatgccc agcgccacgc gccgcaagcg cacaagatag acctgctgcg
     5581 cccgctgtat gcgaactcga tggacatcgc ggagggcaaa agcgtcgcgc agcatttctg
     5641 cgccgactgc catgggccgg ccggcatcag caccatcccc ggcgtgccgg atctggcggg
     5701 ccagcgcgcc gcgtatctgt atagcgaaat gcgtgcctat ctgtccggcg cgcgcggcga
     5761 cgacacgatg aacggggcga tcacctatct cagcgccgac gcgatgagca acgtggccgc
     5821 gtattacggc agcctgccgc ccgccgcgcc ggcgccggcc gcgaagaagg gcgagctggg
     5881 tgcggtggaa gccggcaaag ccgccgccgc cacctgcggc gggtgccatg gcgccaccgg
     5941 catcaccgca acggccggcg tgcccagcct ggtggggcag gagccgaaat acctggtcac
     6001 cgccctgggc gagtacaagg acggctcgcg caagaacgac acgatgaagg cgatggcggc
     6061 gccgctggac agcgtgacca tgagcaacat cgcgctgttc tatgcgctgg agaagccggc
     6121 caaggcgccg accccggccc caggcgatgc ggcggcgggc cagaccgctt cggccccctg
     6181 cgcggcctgt cacggcgcca atggcatcag cggcaacccg gcgacgccca gccttgccgg
     6241 ccaggatgcg cagtacctgg ccgacgccct gcacgggtac aagtccggcg cgcgcgaaaa
     6301 caccaccatg aaggcgctcg cggcaagcct ggacgacgcc acgatcaaga acctggctgc
     6361 gtactacgcc ggcctgcagc cgcagcagcc gaatgtgcgc aagcccctca gcaccgccga
     6421 atgggtggag cgctgcgacc gctgccacgg gcccggcggc aacagcgccg atccgctgat
     6481 gccgtccctg gcatcgcagc gcgccgccta cctggagaaa gtgctgaacg cctatcgcgc
     6541 gggcaagcgc aacagcccgg tgatgtcggc gatgatggac ggcatgtcgg agaacgacgt
     6601 acgcaacctg gcgcaattct acgccagcca gaaagcgcac gcggttgtct atgtgccggt
     6661 tccagcacgc tgacccaaca agcctgctct agtcctccga caggagatgg atcatgaatc
     6721 gcattctgct gaccgccctg gttgccgcgg cccttgggtt cgccggcgcc gctcatgccg
     6781 ctggtgacgc tgcggcaggc aaggccaagg cggcaagctg cgccatgtgc cacggcccca
     6841 atggcgaagg gaacaagatg ggtccgaaac tgaccggcat cgccccggca gccttcatca
     6901 aggcgatgga cgactacaag tccggcgcgc gggccaaccc gatgatgaaa tcagcggcca
     6961 ccccgctcag cgccgacgat atcgcgaacc tggcggccta ctacgcatcg ctgaaatagc
     7021 cgccttccgg cagcggccgg ctgcgggtgg caatcccgca gccgccgcac acgacgacca
     7081 gaccaccacg accactgaca gcgtcgaccc gccgcacagc cgtcaactcc aacagaacct
     7141 cagacaggag tttgctgatg aaccgcattc tactcaccgc cgtcatcgcc ggatcgctcg
     7201 gcctcgccgg cgcggcgcag gccgccggca atgccgcggc gggcaag
//
LOCUS       DASSZH010000301         4665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_433340, whole genome shotgun sequence.
ACCESSION   DASSZH010000301 DASSZH010000000
VERSION     DASSZH010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433340"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1543)
                     /locus_tag="VFN42_06495"
     CDS             complement(<1..1543)
                     /locus_tag="VFN42_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HET8996300.1"
                     /translation="MNQISASDRTARLALVGNPNCGKTALFNVLTGKRQKVANYPGVT
                     VERKEGPLTTPDGRFTRVLDLPGTYSLRARSPDEVITRDAVLGRLAGESPPDLLLCVV
                     SATAPRVGLRLALELKRLGRPMLLVLNMADIARHRGITIDEAQLSAALGVPVVSSVAV
                     RRGGTDRMLSAIDAALRQPSPAPAEAAGWTPPSAAELRAMQREADAILQRAVTEPGRP
                     DTLSARLDAVLLHPVAGLVVLLAILFVMFQAVFIWAQPLMEAITARFTLLGNLVAATL
                     PPGLLASFLRDGVIGGVGSVIVFLPQILILFLFILLLEDLGYMARAAFLMDRIMGGAG
                     LHGRAFIPLLSSFACAIPGIMATRVIDSKRDRLATILVAPLMTCSARIPVYTLIIGAF
                     IPARPVFGGLSLQGLVMFGLYAVGIASALGMSFVFKRVLWRKTPPEPFMLELPDYKLP
                     RLRSVGLGLFQRARMFLRRAGTTIASMMVVIWFLASVPLPPPNATQPAINYSFAARIG
                     HLLEPV"
     gene            complement(1551..1832)
                     /locus_tag="VFN42_06500"
     CDS             complement(1551..1832)
                     /locus_tag="VFN42_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HET8996301.1"
                     /translation="MKPNSAETCCRLGDASPRFRGRIVSVTGALSDSSLPSDELERRL
                     VEMGFVEGASVELRHQGLFRADPIAVQVDGTLVALRRREAQAILVSAGD"
     gene            2220..2522
                     /locus_tag="VFN42_06505"
     CDS             2220..2522
                     /locus_tag="VFN42_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996302.1"
                     /translation="MRLLLALGVAAAFAATAPLGFARTSNTGQTSSNTGSPTYSSPGY
                     HEGGASAQPKQYQRAQAGQSGVYSSPGYHQGGASAQPNQYKRAQAGQTGNAPSAKK"
     gene            2787..4142
                     /locus_tag="VFN42_06510"
     CDS             2787..4142
                     /locus_tag="VFN42_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET8996303.1"
                     /translation="MASTVAHALSAVVNISVVRAASPGSADKPSTGVKFVVGSGFVID
                     PGGIILTNQHVIEGAISIRVTFQDKSQYPAHLIAAASLVDLALLKVEVPHPLPTLAFG
                     DSDRLRIGQPLIAIGNPIGVGTSVSTGSVSALNRNLMRTPFDDFIQTDAAINPGNSGG
                     PLLDCAGKVVGMDTALMSNNQVAGSIGLGFAMPSDDVRLVARKLLNPHNMPNWIGIHL
                     QDLTARLAMLFGRPVVSGAIVTAVDPGSPASEAALVPGDIITGAFGRSLPDSRAIMRA
                     ITVVPPGSPTTLSIWHDGATRQVALRGRPWPNWNKDRRSVVASEADIRRSLAAGLGVT
                     LRSISEADRIQFHLGGIEGVLVDRVRPGSQADMLGIKRGDVIQQVGHQPARTPAQVSA
                     ALSYGKPDADDVVAALVHAPAGAKWVTFWVGNIRSPDLVTGTPLPQASPSTQSAAAQG
                     R"
     gene            complement(4143..>4665)
                     /locus_tag="VFN42_06515"
     CDS             complement(4143..>4665)
                     /locus_tag="VFN42_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HET8996304.1"
                     /translation="VIDIDAEFAAAGLKSAPEESSLLAVSNGTSALIYLHPDRAADAD
                     AVLGFLNDQAWAGAVLAGDDLRAIGQAPEGALLCAVSMHSDDRTNAYGVPGISAAARP
                     TVGKPDRLGCGQHGGLGAYEQMPFLMIQGLGFGRGSVGVEPSSVIDLAPTILAHLGLP
                     AEGCEGKALQGRV"
BASE COUNT          794 a         1584 c         1589 g          698 t
ORIGIN      
        1 gcaccggttc cagcagatgg ccgatgcggg cggcgaagct gtagttgatc gcgggttgcg
       61 tcgcgttggg cggcggcagc ggcaccgagg cgaggaacca gatcaccacc atcatgctgg
      121 caatggtcgt gccggcgcgg cgcaggaaca tccgggcccg ctggaacaag cccagcccca
      181 cgctgcgcag gcgcggcagc ttgtagtccg gcagttccag catgaacggc tcgggcgggg
      241 tcttgcgcca cagcacgcgc ttgaacacga aggacatgcc gagggccgag gcgatgccca
      301 ccgcgtacag gccgaacatc accaggccct gtagactcag cccgccgaac accggcctcg
      361 cggggatgaa ggcgccgatg atcagcgtgt acaccgggat gcgggcggag caggtcatca
      421 gcggcgccac caggatggtg gccagccgat cgcgcttact gtcgatcacc cgcgttgcca
      481 tgatgccggg aatggcgcag gcgaagctgg acagcagcgg gatgaaggcg cgcccatgca
      541 gcccggcacc gcccatgatg cggtccatca ggaaggcggc gcgggccatg tagcccaaat
      601 cttccagcag caggatgaac aggaacagga tcaggatctg cggcaggaac acgatcacgc
      661 tgccaacgcc gccgatcacg ccgtcgcgca ggaagctggc gagcagcccc ggcggcagcg
      721 tcgcggcgac caggttgccc agcagggtga acctggcggt gatcgcctcc atcagcggct
      781 gtgcccagat gaacaccgcc tggaacatga cgaacaggat cgccagcaga accaccaggc
      841 cggcgaccgg atgcagcagc accgcatcca gccgtgcgct caacgtatcc ggccggcccg
      901 gttcggtgac cgcgcgctgc aggatcgcgt ccgcctcgcg ctgcatcgcg cgcaattccg
      961 ccgcgctggg cggggtccag ccggctgcct cggccggcgc cgggcttggc tgccgcaggg
     1021 cggcgtcgat cgccgacagc atccggtcgg tgccgccacg ccggaccgcc accgaggaaa
     1081 ccaccggaac ccccagcgcg gccgaaagct gcgcctcgtc gatggtgatg ccacggtgcc
     1141 gggcgatatc cgccatgttc agcaccagca gcatgggacg gccaagccgc ttgagttcca
     1201 gcgccaggcg caggccgacc cgcggcgcgg tggcgctgac gacacacagc aacaggtcgg
     1261 gcggcgactc accggcaagg cgcccgagca cggcgtcacg cgtgatcacc tcgtccgggc
     1321 tgcgggcgcg cagcgagtag gtgccgggca ggtcgagcac gcgggtgaag cggccgtccg
     1381 gcgtggtcag cgggccttcc ttccgctcca ccgtcacgcc cgggtagttg gcgaccttct
     1441 gccgcttccc cgtgagcacg ttgaacaggg cggtcttgcc gcagttcgga ttgccgacca
     1501 gcgctaggcg cgcggtacgg tcgctggccg aaatctggtt cacggcaagc tcagtcgccg
     1561 gcggaaacga ggatggcctg cgcctcccgc cggcgcagcg cgaccagcgt gccgtccacc
     1621 tgcacggcga tcggatcggc gcggaacagg ccctggtggc ggagttcgac cgaggcgccc
     1681 tcgacaaagc ccatctccac caaccggcgc tccagctcgt cggagggcag cgaactgtcg
     1741 gacaaggctc cggtcaccga gacgatccgg ccgcgaaagc ggggggatgc gtcgccgagg
     1801 cggcaacagg tttcggcgga gttgggcttc atggccggct ctcatgcaac gggcgggacg
     1861 gagttaatgc gaatagctcg caattgcaag tgccggatgc cggccgccgc gctgccccgc
     1921 ggtcgcccgg tccgatccac cgctggtggg gtcgcgggag gctcgccggc gggcatgaaa
     1981 gtccggtccc ccggccgcag cacgtccgca ctctcctgtg ccaggtcgta tcagcggaaa
     2041 ccaaccggca gatggcgcac gcaggctgca cgtgcaccgg caaggtgcgg gaaccggacg
     2101 caagcgtact ttggccggca ggcatcgcga acattgtctg gagcggagcg gaacttcgca
     2161 acccgcagtc gcgctgcgcg ttgacatgac tcgccgatcc acaagagagg ggttctaaca
     2221 tgcggctact actggcattg ggtgtcgctg cggccttcgc cgcgaccgcc ccgttgggct
     2281 ttgcccgcac ctccaacact ggccagactt cgtccaacac aggcagccct acctacagca
     2341 gcccgggcta ccatgaggga ggcgcgtccg ctcagccgaa gcagtaccag cgggcccaag
     2401 ccggccagag tggggtctac agcagccccg gctatcacca gggcggcgcc tccgcgcagc
     2461 cgaaccagta caagcgggcc caggccggcc agactggcaa cgcaccgtcc gcgaagaagt
     2521 agatcgaacg ggcagtcgcc tcgcggccga ggctgcgtct caatgcgggc tgcgtcacga
     2581 ggcggctctt tccgaggcgt tgcaccgccc ctgtcttccg gcggccgcgc ctcatgccgg
     2641 ggttggaggc aggcctgacg acacacgggc cgaaacggcc gttgcctgcg ggccgcaggg
     2701 cgctgctctg cgggctgctg ctggctgccc tggcgtttcc gctgctgtct ccggcgggag
     2761 cggcaacccc cgcatcggcc tgcgatgtgg cgagtaccgt cgcgcatgct ctctcagccg
     2821 tcgtcaacat atcggtggtg cgtgctgcgt cgcctggcag cgcggacaaa ccctccaccg
     2881 gcgtcaagtt cgtcgtcggc agcggattcg tcatcgaccc gggcggcatc attctgacga
     2941 accagcacgt cattgaaggc gcgatctcga tccgggtgac gtttcaggac aagtcccagt
     3001 atccggcgca tctgattgcc gcggcttcgc tggtggacct ggcattgttg aaagtggagg
     3061 ttccccatcc gctgccgacg ctggcgttcg gcgacagcga tcgcttgcgg attggccagc
     3121 cactgattgc catcggcaac ccaatcgggg tgggcacgtc ggtgagcacc ggaagcgtca
     3181 gcgcgctcaa ccgcaatctg atgcgtaccc cgttcgacga cttcatccag accgatgcgg
     3241 cgatcaatcc aggcaactcg ggtgggccgc tgctggactg tgccggaaaa gtggtcggca
     3301 tggacacggc gttgatgtcg aacaaccagg tggcggggtc gatcggactc ggatttgcca
     3361 tgccgtccga cgacgtcagg ctggtcgccc gaaagttgct caacccgcac aacatgccga
     3421 attggatcgg catccatctt caggatctca cagccagact ggccatgctg ttcggccggc
     3481 ccgtggtgtc cggcgcgatc gtcaccgcgg tggatcccgg cagcccggca tccgaagccg
     3541 cgctggttcc cggcgatatc atcaccggcg cattcggccg gagccttccc gattcgcgtg
     3601 ccatcatgcg ggcgatcacc gtcgtgccgc ccggtagtcc gacgacgctt tccatatggc
     3661 acgacggtgc gaccagacag gtggcgctgc gtggccgccc gtggccgaac tggaacaagg
     3721 atcggcgttc cgtggtggcc agcgaagccg atatccgccg ctcgctggcg gccgggttgg
     3781 gcgtgacgct ccgctcgatc agcgaagctg accggatcca attccatctg ggaggcatag
     3841 agggcgttct ggtcgatcgg gtccgcccgg gctcgcaggc cgacatgctt ggcatcaagc
     3901 gtggcgatgt cattcagcag gtggggcatc agccggctcg cacgccggca caggtcagcg
     3961 cggcgctgag ttatggcaag cccgatgccg acgacgtggt cgccgccctg gtgcatgcgc
     4021 ctgccggcgc caaatgggtg actttttggg tgggcaacat ccgttcaccg gatctggtca
     4081 ccggcacgcc tttgccccag gcaagcccat cgactcagag cgcagccgcg cagggccgct
     4141 gatcacaccc ggccctgcaa ggctttgcct tcgcaaccct ccgccggcag acccagatgc
     4201 gccaggatcg tgggggcaag gtcgatgacc gagctgggct ccaccccaac gctgccgcgt
     4261 ccgaagccca ggccctggat catcaggaat ggcatctgct cataggcgcc caggccgcca
     4321 tgttgcccac agccgagccg gtcgggcttg ccgacggtgg gacgcgcggc ggcgctgatg
     4381 ccgggaaccc cataggcgtt cgtccgatcg tcgctgtgca tggagacggc gcagagcagg
     4441 gccccttccg gtgcctggcc gatggcgcgc agatcgtctc ccgccagcac ggcgccggcc
     4501 caggcctggt cattgaggaa cccgagcacg gcgtccgcat cggccgcccg atccggatgc
     4561 aggtagatca gcgcggaagt tccatttgaa acggcaagca ggctcgactc ctccggtgcg
     4621 gatttcagcc ctgccgcggc gaattccgca tcgatgtcga tgacc
//
LOCUS       DASSZH010000302         2484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_637987, whole genome shotgun sequence.
ACCESSION   DASSZH010000302 DASSZH010000000
VERSION     DASSZH010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637987"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..1072)
                     /gene="argF"
                     /locus_tag="VFN42_06520"
     CDS             complement(110..1072)
                     /gene="argF"
                     /locus_tag="VFN42_06520"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET8996305.1"
                     /translation="MSASLKRSVGQHDHPADRPGLRHFLDLRDFDPATLRQMLDVAGN
                     LKRGGTSSRPLAGKTLALIFEKPSTRTRVSFEVGMRQLGGDVVVLSARDMQSGRGESP
                     ADTARVLSRYVDAIMLRTDNVAKLHELAGYATVPVINGLTDTSHPCQLMADVLTFEEH
                     RGPIAGQVIAWCGDGNNVARSWIEAAVRFGFTLRLATPDALRPPQEVVDWARAQGGRI
                     ELTDDAEKAVAGARCVVTDTWVSMSDDPNENRHNLLAPYRVTASLMKQAAADAIFMHC
                     LPAHRGEEVTEEVIDGPQSVVFDEAGNRLHAQKGVLAWVLGAVR"
     gene            complement(1069..2265)
                     /locus_tag="VFN42_06525"
     CDS             complement(1069..2265)
                     /locus_tag="VFN42_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET8996306.1"
                     /translation="MIPALLPNYARADLAFERGEGAWLWTVDGRRFLDFGSGIATASL
                     GHGHPHLSKAIAEQAAKVMHVSNLYRIPQAETLAQRLVDASFADSVFFCNSGAEANEG
                     MIKMMRRTMHDAGKPERFRFITFEGAFHGRTLATLAATGNAKYLEGFGPAVDGFDQVP
                     FNNMNAVRNAVGPNTCGIIVEPVQGEGGVRPADMQFLRDLRAACDEYGIILGMDEVQS
                     GMGRTGKLFAHEWAGITPDVMSVAKGIGGGFPLGAVLAKEQFAKALKPGTHGTTFGGN
                     PLACAAGNAVLDIILAPGFLDEVKRKGDRLHAELAEIAREYPQVYADARGMGLLQGLK
                     CVLPQGEVQAACVAEGLMAITAGDNVLRLAPPLVVTDADLDEAIVMLRRATRRVLPSD
                     GKAAAK"
BASE COUNT          378 a          862 c          841 g          403 t
ORIGIN      
        1 agccggccac cctggcgctc gccatgcggc aggacgcttg cactccgcac gcagacggcc
       61 ctgcgcccgc caggacgcag gggccgcgcg caacgacgcc gctcctctgt taacgcaccg
      121 cgccgagcac ccaggccaac acgcctttct gcgcgtgcag ccggttcccg gcctcgtcga
      181 acaccaccga ttgcgggccg tcgatcacct cctcggtgac ctcttccccg cggtgtgccg
      241 gcaggcagtg catgaagatc gcatccgctg ccgcctgctt catcagcgac gcggtcaccc
      301 ggtacggcgc cagcaggttg tggcggtttt cgttcggatc gtccgacatg ctgacccagg
      361 tatcggtcac cacgcagcgc gcgccggcca ccgccttttc cgcgtcatcc gtcagctcga
      421 tccggccgcc ctgggcgcgc gcccagtcca cgacctcctg tggcggccgg agtgcatccg
      481 gcgtggcgag ccgcagcgtg aagccgaagc gcaccgccgc ttcgatccag ctgcgtgcga
      541 cgttgttgcc gtcgccgcac caggcgatta cctgcccggc gatcggaccg cgatgctcct
      601 cgaaggtcag cacgtcggcc atcagctggc agggatgcga ggtgtcggtg aggccgttga
      661 tcaccggcac cgtggcgtag cccgccagtt cgtgcagctt cgccacgttg tcggtgcgca
      721 gcatgatcgc atcgacatag cgcgacagca cgcgcgcggt atcggcgggg gattcgccgc
      781 gcccgctttg catatcgcgg gccgacagca ccaccacgtc gccgcccagc tggcgcatgc
      841 cgacttcgaa ggaaacccgc gtccgggtgg acggcttttc gaagatcagc gccagcgtct
      901 tgccggcgag cgggcgcgag gaggtgccgc cgcgcttcag gttgcccgcg acgtccagca
      961 tctgccggag cgtggcggga tcgaaatcgc gcaggtcgag gaagtggcgc aggcccgggc
     1021 ggtccgccgg atgatcgtgc tgtcccacgc tgcgcttcag cgacgcgctc atttcgccgc
     1081 ggccttgccg tcggagggca gcacgcggcg ggtggcgcgg cgcagcatca cgatcgcctc
     1141 gtccaggtcg gcgtcggtca ccaccagcgg cggcgccagg cgcagcacgt tgtcgccggc
     1201 ggtgatggcc atcagccctt cggcgacaca ggcggcctgc acctcgcctt gcggcagcac
     1261 gcatttgagt ccttgcagca gacccatgcc gcgcgcgtcc gcatagacct gcgggtattc
     1321 gcgggcgatc tccgccagct cggcgtgcag gcggtcgccc ttgcgcttca cctcatccag
     1381 gaaaccgggc gccaggatga tgtccagcac ggcgttgccg gcggcgcagg ccagcgggtt
     1441 gccgccgaag gtggtgccgt gcgtgccggg cttcagcgcc ttggcgaact gctccttggc
     1501 cagtacggcg cccagcggga agccgccgcc gatccctttc gccacagaca tgacgtccgg
     1561 cgtgatgccg gcccattcat gcgcgaacag cttgccggta cggcccatgc cggactgcac
     1621 ctcgtccatg cccaggatga tgccgtactc gtcgcaggcc gcgcgcagat cgcgcaggaa
     1681 ctgcatgtcg gcgggacgca cgcccccttc gccctgcacc ggctcgacga tgatgccgca
     1741 ggtgttgggg ccgaccgcat tgcgcaccgc gttcatgttg ttgaacggca cctggtcgaa
     1801 gccatccacc gccgggccga agccttccag gtatttcgcg ttgccggtgg cggcgagcgt
     1861 cgccagggtg cggccgtgga acgcgccttc gaaggtgatg aagcggaacc gctccggctt
     1921 gccggcatcg tgcatggtcc ggcgcatcat cttgatcatg ccctcgttcg cttccgcgcc
     1981 cgagttgcag aagaacacgc tgtcggcgaa gctcgcatcg accagccgct gcgccagggt
     2041 ttccgcctgg ggaatacgat acaggttcga aacgtgcatc accttggccg cctgttcggc
     2101 gatggccttg ctcagatgcg gatggccgtg gccaagcgaa gcggtggcga tgccggaacc
     2161 gaaatcgagg aatcgtcgcc cgtccaccgt ccacaaccag gcgccttcgc ctctctcaaa
     2221 ggcgaggtct gcacgggcgt agttcggcag cagagcggga atcatgggga taagaccctc
     2281 gttgggatgt ttccaaatgg cgcattcgcc cgcagccggt tggaccgggt gcaggcgaag
     2341 cgcggatcat tggcgggggt tggggccggt gtcaaacccg tgagggcgcc gccggtgcgc
     2401 ggccggaaac cgcagcgcgg actgccggcc ggtgcggggc ggcaaaatcg taacgcgagc
     2461 ttattgtgtc gctcaaggaa caca
//
LOCUS       DASSZH010000303         3968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_518667, whole genome shotgun sequence.
ACCESSION   DASSZH010000303 DASSZH010000000
VERSION     DASSZH010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518667"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="VFN42_06530"
     CDS             complement(<1..1034)
                     /locus_tag="VFN42_06530"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET8996307.1"
                     /translation="MNSMILLQNNLAATLAVAANVTPSLSDSYWGLVETSAAAEQNKP
                     LFWVSRNAGITDGVTFTFSATAQIGGVTVTLGVQLAGTLLGSDIKISVQAGEQTSGWS
                     PDADVSLAFAGSDGNTYRVTGAYVGEGAQYDNVQFAVSATVLPRIKHVVVLMFENRSF
                     DNLLGWLYDGPAEAPAHFVPDNTPHVFNGLKADTYANAAPKVHNGEPVPATKGTTEWT
                     VGDRTVPASCVPDPDPGEEFLHCRTQIGKAMDGFLADYLLSFPGDESLDSAAQIMQSY
                     APAQVPIITSLAKAFAVSDAWHASVPSQTWANRAFAQAGASAGHVNNQGWPWNIPTIF
                     DLLEAQGLSWR"
     gene            complement(1172..2800)
                     /locus_tag="VFN42_06535"
     CDS             complement(1172..2800)
                     /locus_tag="VFN42_06535"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET8996308.1"
                     /translation="MTAAIAARAAGASVIVVEQEPDIGGHAITSGGNVPLGGGTTAQR
                     RHGIADDPELLFRDLTDWSVVEPNGFPDYRYNDREIVRAFVDHNVETFDWLVAHGVVF
                     VDKAPDARGGNAVGNSAPREMHCAVSHWPTVQTGRPAPLEEQATRSSGNGLMRPLEAA
                     ARKAGVEILLRHRMTGLYRETPTAGGVLGIAVEHAGAPLAIRARRAVIIGTGGSTGNV
                     HFRRMVDPRLTEEYCGLAGMPWSDQDASGEMAAMAIGASLWGMYNQTGEFGFNLTKPV
                     GIGCQYGYQNLRWYPGSAVFDKAGAIGLSVKDWQNVITVNMLGQRFYDETGPGFTTNN
                     YKSLEPYTQGSWRNVQNVTFRPNNWLNAAMAGIGDGHNGGGPIWAIFDAEAARREEWL
                     TEPPYVDREAGFFFSADTLAGLAQRIVMRHQRVPMPPDNLERTVARYNSFVESGVDAD
                     FAKPQPRYKIATPPFFAAWATPVIHDTRMGLRIDAQCRVIDLHGRVIPGLYCGGESAG
                     GFSQHGLARAICQGIIAARHAVQRTAAESEQRGA"
     gene            complement(2766..2849)
                     /locus_tag="VFN42_06540"
                     /pseudo
     CDS             complement(2766..2849)
                     /locus_tag="VFN42_06540"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3069..>3968
                     /locus_tag="VFN42_06545"
     CDS             3069..>3968
                     /locus_tag="VFN42_06545"
                     /inference="COORDINATES: protein motif:HMM:NF044428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996309.1"
                     /translation="MTELYWLPTVPDWRDRLRALPDEPLMAWEHAVALANARLNFVQT
                     NSLDAALRQRVTAPPAGIATKPERLAVLGSATLAHLLPAIRVGGLRRGIWIDTYEGEF
                     GQYMQELADPPDALRDFAPTAVLLALDAHHLTAELHPSMDSAEAEAALAATVSRVRDA
                     WRQARAAFRCPVIHQAALPLHLPLLGHNEHRLPGSRAGALTRLNAALREAADADGVDI
                     LALDDRAAVDGLLAWHKPTLWHHAKQEISPVAAPLYGDLVARLLAARQGRVGKCLVLD
                     LDNTLWGGVIGDDGLEGIVLGQGS"
BASE COUNT          577 a         1404 c         1347 g          640 t
ORIGIN      
        1 cgccaggata gcccctgcgc ttcgagcaga tcgaagatag tgggaatgtt ccagggccag
       61 ccttggttgt ttacatgacc cgcggatgct ccggcctggg cgaaggcccg gttcgcccag
      121 gtctggctcg gcacggacgc atgccaggcg tcggataccg cgaaggcttt cgccaggctg
      181 gtgatgatgg gaacttgcgc cggcgcataa ctttgcatga tctgtgcggc gctatcgaga
      241 gactcgtccc ccgggaacga gagcaggtag tcggccagga agccgtccat cgccttgccg
      301 atctgcgtcc ggcaatgcag gaactcttcg cccggatcgg gatccggcac gcaactggcc
      361 ggcacggttc tgtcgcccac cgtccactcg gtggtgccct tggtcgcggg caccggctct
      421 ccattgtgaa ccttcggcgc ggcgttcgcg taggtatccg ccttcaggcc gttgaacacg
      481 tgcggcgtgt tgtccggaac aaaatgggcc ggtgcctctg cggggccgtc gtagagccaa
      541 cccagaagat tgtcgaagga ccggttctcg aacatcagca cgacgacgtg cttgatccgc
      601 ggcaggacgg tggcactgac cgcgaactgc acgttgtcgt attgcgcgcc ttcgccgaca
      661 taggcaccgg tgacccggta ggtgtttccg tcgctgccgg cgaaggccag cgagacgtcg
      721 gcgtcaggcg accagccgct ggtctgctcg cccgcctgca ccgaaatctt gatgtcgctg
      781 ccgagcagcg taccggccag ttgcacgccc agcgtgaccg tgacgccacc gatctgcgcc
      841 gtggcactga aggtgaaggt cacgccatcg gtgatgccgg cgttgcggct gacccagaac
      901 agcggcttgt tctgctcggc cgcggcgctg gtctcgacca gcccccagta gctgtcgctg
      961 aggctgggcg tgacattcgc cgccactgcg agcgtcgccg caagattgtt ctgcagcagg
     1021 atcatcgagt tcatggttgc cccccgatcg tttgctgccg ctgcccgtct ttgcgcgcca
     1081 gtctgacaca ttccgcccgg gacggcgacg gctgggcgtg acgggcgcgg gggcgacgtt
     1141 ccacgcgttg gtcagcgcaa accgccgcga ttcacgcgcc gcgctgttca gactctgccg
     1201 cggtccgctg caccgcatgc cgcgcggcga tgatcccctg gcagatcgcg cgcgccagcc
     1261 cgtgctggct gaagccaccg gccgattccc cgccgcaata caggccggga atgacccggc
     1321 cgtgcagatc gatcacccgg cattgtgcgt cgatccgcag acccatgcgc gtgtcatgga
     1381 tcaccggcgt ggcccaggcg gcaaagaagg gcggggtggc gatcttgtag cgaggctggg
     1441 gcttggcgaa atccgcgtcg acgccggatt ccacgaagct gttgtagcgt gccacggtgc
     1501 gttccagatt atccggcggc atgggcacgc gctggtggcg catgacgatc cgctgcgcca
     1561 gcccggcgag cgtgtcagcg ctgaagaaga acccagcctc ccgatcgaca tagggcggtt
     1621 cggtcagcca ttcctcgcgc cgagcggcct cggcgtcgaa gatcgcccag atcgggccgc
     1681 cgccattgtg gccgtcgccg atccccgcca tggccgcgtt cagccagttg ttggggcgga
     1741 acgtcacgtt ctgcacgttg cgccagcttc cctgggtata gggctccagc gacttgtagt
     1801 tgttcgtggt gaagccgggg ccggtctcgt catagaaccg ctgacccagc atgttcacgg
     1861 tgatgacgtt ctgccagtcc ttcaccgaca ggccgatcgc gcccgctttg tcgaacaccg
     1921 cgctgccggg ataccagcgc aggttctggt agccgtattg gcagccgatc cccaccggct
     1981 tggtcaggtt gaagccgaac tcgccggtct ggttgtacat cccccagagc gacgcaccga
     2041 tcgccatggc tgccatttcg ccgctggcgt cctggtccga ccagggcatg ccggcaagac
     2101 cgcaatattc ctcggtcagc cgcggatcga ccatgcggcg gaagtgcacg ttgccggtgg
     2161 agccgccggt gccgatgatc accgcccggc gagcccgaat ggccagcggg gcgccggcat
     2221 gctccaccgc gatgccgagc acgccgccgg cggtgggggt ttcgcgatac aggccggtca
     2281 tgcggtggcg cagcaggatc tccaccccgg ccttgcgtgc cgccgcctcc agcggccgca
     2341 tcaggccgtt gccggaggag cgtgtcgcct gctcctccag cggcgcgggc ctgccggtct
     2401 gcacggtagg ccagtgcgac acggcgcaat gcatctcacg cggcgccgaa ttgcccaccg
     2461 cattgccgcc gcgcgcatcc ggtgccttgt ccacgaacac cacgccatgc gcgaccagcc
     2521 agtcgaacgt ctccacgttg tggtccacga aggcgcggac gatctcgcgg tcgttgtagc
     2581 ggtagtcggg gaagccgttc ggttccacca ccgaccagtc ggtcaggtcg cggaacagca
     2641 gttccgggtc atcggcgatg ccgtgccgcc gctgcgccgt ggtgccgccg cccagcggca
     2701 cgttgccgcc gctggtgatc gcgtggccgc cgatatccgg ctcctgctcc accacgatga
     2761 ccgaggcccc ggccgcccgc gcggcgatcg ccgcggtcat gccggtggcg ccggaaccga
     2821 tcaccaccac gtccgccgtg gcatcccatg cggcgggcgg ggccggcgcg gcgaggctta
     2881 tgtccgcgcg ggtggaagcg ccccctgatt cgtctgccat gcgttgcgtc ccggtctttc
     2941 cgcttccgaa ccacgccggg gtgatgccgt caacggtcca cccctttcgc ttggacgcgc
     3001 cgcgcgcgcc cgggctatac tcccgatcgc gcgagcacgc gcaccgacct tggcaggacc
     3061 tcgcccgcgt gaccgagctt tactggcttc ccaccgttcc ggactggcgc gaccggctgc
     3121 gcgcgctgcc ggacgaaccg ctcatggcgt gggagcacgc ggtggccctg gccaacgccc
     3181 ggctgaattt cgtgcagacc aacagcctgg acgccgcgct gcgccagcgc gtgaccgcgc
     3241 cgcccgccgg catcgccacg aagccggagc ggctggcggt gctgggcagc gccacgctgg
     3301 cccacctgct gcccgccatc cgcgtcggcg ggctgcggcg cggcatctgg atcgacacct
     3361 atgaaggcga attcggccag tacatgcagg agctggcgga cccgcccgac gcgctgcgcg
     3421 atttcgcccc caccgccgtg ctgctggcgc tggacgcgca ccacctgacc gccgagctgc
     3481 accccagcat ggactcggcg gaggccgagg ccgcgctggc cgccaccgtc agccgcgtcc
     3541 gcgacgcctg gcgccaggcg cgggcggcgt tccgctgccc cgtgatccac caggccgcgc
     3601 tgccgctgca cctgccgctg ctggggcaca acgaacaccg gctgcccggc tcccgcgccg
     3661 gcgcgctgac ccggctgaac gccgccctgc gggaggccgc cgacgccgac ggcgtggaca
     3721 tcctggcgct ggacgaccgc gccgcggtag acgggctgct cgcctggcac aagcccacgc
     3781 tgtggcacca cgccaagcag gagatcagcc ccgtcgccgc gccgctctac ggcgacctgg
     3841 tcgcgcgcct gctcgccgcc cggcagggca gggtggggaa atgcctggtg ctggacctgg
     3901 acaacacgct gtggggtggc gtgatcggcg atgacgggct ggagggcatc gtcctgggcc
     3961 aaggcagc
//
LOCUS       DASSZH010000304         2971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_160753, whole genome shotgun sequence.
ACCESSION   DASSZH010000304 DASSZH010000000
VERSION     DASSZH010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160753"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="VFN42_06550"
     CDS             <1..860
                     /locus_tag="VFN42_06550"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HET8996310.1"
                     /translation="AALGLAASFASGLKANFGTMTKPMHVGHTARNGLLAALMAREGF
                     TANPAALEHQQGFLDVFNGDGTYETGRILADWYAPFECEGGEPGLKPYPCCGSTHASI
                     DRMLDLAQRHDLRPEQIEGIEVMPHARRLPHTNNPDPRTPLAAKFSIQYCVARALADR
                     AVRLEHFEHDAHLDPDIRVLTARVQARPHPALTEDTAQWGAEVVVTTRDGRRLASRID
                     GYERRAPDAKAMTQDELWVKFADCAQRALPQARIAPAFERLSRIDSLACITELTALLE
                     LRAGTAQAA"
     gene            879..1337
                     /locus_tag="VFN42_06555"
     CDS             879..1337
                     /locus_tag="VFN42_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET8996311.1"
                     /translation="MTADERFAALGLALPPPPKPLGNYVPFRLAGNMLYLSGVGPRGA
                     DDSTIIGKVGDNLSVEQGYEAAKLCGLNLLTNMIAAVGKLERIDTVLKVFGMVNATPD
                     FGRHPEVINGCTDLFVAVFGDAGRPARSAVGMGSLPRNIAVEIEAIALVR"
     gene            complement(1362..1856)
                     /locus_tag="VFN42_06560"
     CDS             complement(1362..1856)
                     /locus_tag="VFN42_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996312.1"
                     /translation="MIARAVLPLLAAGLLLSGCKAVPQIAGVVTGGAIGVATASPALG
                     FAVGVATDTATTAGLQWYGRSRANAEQNAIARIAGGLELGQQHAWHIHHIIPFGDEHG
                     ELYVVRVIAAPLTVCKQIVFSVDDGQGAALHQAWYVASVCRQGQDWKWADAEPAVSRW
                     GYLQ"
     gene            complement(1853..2398)
                     /locus_tag="VFN42_06565"
     CDS             complement(1853..2398)
                     /locus_tag="VFN42_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996313.1"
                     /translation="MSGWIGCGNGYRVLRQACVLLPGLCALAGCGPLLSAGTSDVAGV
                     AGAGVASAFGHSPAAATGIGLGIAAGSNAALQYVERDVHGVEQDRIAQAAGKLQPGMI
                     GTWGVVHDIPIEPNEHGEVMVTSVIGSPAFSCKQIVFSVDTMQDKQVSRRFYTATVCL
                     DGKAWKWATAEPATARWGALQ"
     gene            complement(2465..>2971)
                     /locus_tag="VFN42_06570"
     CDS             complement(2465..>2971)
                     /locus_tag="VFN42_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8996314.1"
                     /translation="ERDQRERFRSEAEAIAEFIGQNGMQAFAAKYAYGPTRVQFENKD
                     PRGFAEFRQNLSEHSALGARNTQLGVQRERPSLYDLIDEMKALTVPALILTGDEDWPC
                     LQPGLLMKQHIRSAALSVMPNCGHAINLEGPDEFNRIVGSFMAQVDAGRWPMRDPRAM
                     TASITGMR"
BASE COUNT          495 a         1048 c          955 g          473 t
ORIGIN      
        1 ccgcggcact cggcctcgcg gcttcctttg ccagcggcct gaaggcgaat ttcggcacca
       61 tgaccaagcc gatgcatgtc ggccacaccg cccgcaacgg cctgctcgcc gcgctgatgg
      121 cacgcgaagg tttcaccgcg aatccggccg cgctggaaca ccagcaggga ttcctcgacg
      181 tgttcaacgg cgacggcacc tacgagaccg gacgcatcct ggcggactgg tacgccccgt
      241 tcgaatgtga aggcggcgaa ccggggttga agccgtatcc gtgctgcggc agcacgcatg
      301 cctcgatcga ccgcatgctg gatctggcgc agcgccacga tctgcggcct gagcagatcg
      361 aaggcatcga ggtcatgccg catgcgcgcc gcctgccgca caccaacaac cccgatccgc
      421 gcacaccgct cgccgcgaaa ttcagcatcc agtactgcgt cgcgcgtgcg ctggcggacc
      481 gcgcggtgcg gctggagcat ttcgagcacg acgcccatct ggatccggac atccgcgtgc
      541 tgacggcgcg cgtgcaggcc aggccacatc cggcgctgac ggaggacacc gcgcagtggg
      601 gtgccgaggt ggtggtcacg accagggacg gacgccggct ggcctcccgc atcgatggct
      661 atgaacggcg cgcgccggat gccaaggcga tgacgcagga cgaactctgg gtgaaattcg
      721 ccgactgtgc gcagcgtgcg ctgccgcagg cacggattgc gccggcgttc gagcggttga
      781 gccggatcga ctcgctggcg tgcatcacgg agctgaccgc attattggaa ctgcgcgccg
      841 gcaccgccca ggccgcctga acgcaaggag acaacaggat gaccgctgac gaacgtttcg
      901 ccgcgttggg attggctctg ccgccgccgc ccaagccgct gggcaactat gtgccgttcc
      961 gcctggccgg gaacatgctg tacctgtccg gcgtcggccc gcgtggcgcg gacgattcga
     1021 cgatcatcgg caaggtcggc gacaacctgt cggtggagca gggctatgag gcggcgaaac
     1081 tctgcggcct gaatctgctg accaacatga tcgcggcagt gggcaagctg gagcgcatcg
     1141 acaccgtgtt gaaggtgttc ggcatggtca acgccacccc cgatttcggc cggcacccgg
     1201 aggtgatcaa cggctgcacc gatctgttcg tggcggtgtt cggcgatgcc ggccggcccg
     1261 cacgctccgc cgtcggcatg ggcagcctgc cgcgcaacat cgcggtggag atcgaggcga
     1321 tcgcgctggt gcgttgaccc ggtccggcag gacaggacgc gctactgcaa gtacccccag
     1381 cgtgatacgg cgggttccgc gtccgcccac ttccagtcct gcccctggcg acataccgac
     1441 gccacgtacc atgcctggtg caatgcggcg ccttgaccat catccaccga aaacacgatc
     1501 tgcttgcaga ccgtcagcgg cgccgcgatc acgcgcacga cgtacagctc gccatgctca
     1561 tcgccgaacg gaatgatgtg gtggatgtgc caggcgtgct gctgcccgag ttccaacccg
     1621 ccggctatgc gcgcgatcgc attctgctcc gcattggcgc gcgagcggcc gtaccactgc
     1681 agcccggcgg tggtcgcggt atccgtcgcc acgccaaccg cgaagccgag tgccgggctc
     1741 gcggtggcga cgccgatcgc gcccccggtc accacgccgg cgatctgcgg caccgccttg
     1801 cagcccgaca gcaacaatcc cgcggcgagc agcggcagca cggcgcgcgc aatcactgca
     1861 gcgcgcccca gcgcgcggtg gccggttccg ccgtcgccca tttccaggcc ttgccgtcca
     1921 ggcacacggt ggcggtgtag aaccggcgcg aaacctgctt gtcctgcatc gtgtctaccg
     1981 agaacacgat ctgcttgcag gagaatgccg gcgaaccgat cacgctggtg accatcacct
     2041 cgccgtgctc gttcggctcg atcgggatgt cgtgcacgac accccaggtg ccgatcatgc
     2101 ctggctgcag ttttcccgcg gcctgcgcga tgcgatcctg ctccaccccg tgcacgtcac
     2161 gctcgacata ttgcagggcg gcattggatc cggccgcaat gcccaggccg atgccggtgg
     2221 cggcggccgg gctatggccg aacgcgctgg ccacccccgc accggcgacg ccggcgacat
     2281 cggatgtccc cgccgacagc agcggcccgc atcccgccag cgcacacagg ccgggcagca
     2341 gcacacaggc ctgccgcaac acccggtagc cgttgccgca cccgatccat ccagacatgg
     2401 cctgcaacta ggcatgccat gcacgccggg tccaatcaca cgcgtgatgg tatggccgcc
     2461 gggatcatcg catcccggtg atcgaggccg tcatcgcgcg cggatcgcgc atgggccagc
     2521 gtccggcatc cacctgcgcc atgaagctcc ccacgatgcg attgaattcg tcggggccct
     2581 ccaggttgat cgcgtggccg caattcggca tcaccgacag cgccgcactt cttatatgct
     2641 gcttcatcag caggcccggc tgaaggcagg gccagtcctc gtcaccggtc aggatcagcg
     2701 ccggcacggt cagcgccttc atctcgtcga tcaggtcgta cagcgagggc cgttcccgct
     2761 gcacccccaa ctgcgtgttg cgcgcaccaa gcgccgagtg ttccgacagg ttctgcctga
     2821 attccgcgaa gccgcgcggg tccttgtttt cgaattgcac ccgggtcggg ccataggcgt
     2881 atttcgcggc gaaggcctgc atgccgttct gcccgatgaa ttccgcgata gcctccgcct
     2941 cactgcggaa ccgttcccgc tggtcgcgtt c
//
LOCUS       DASSZH010000305         2148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_143589, whole genome shotgun sequence.
ACCESSION   DASSZH010000305 DASSZH010000000
VERSION     DASSZH010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143589"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VFN42_06575"
     CDS             complement(<1..288)
                     /locus_tag="VFN42_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HET8996315.1"
                     /translation="MPPFPDLPVTDALPRLAATLAAGNNAVLVAPPGAGKTTLVPLAL
                     RDAPWVAGGRIVMLEPRRLATRAAATRMAALLGEPVGRTVGYRTRLDSAVSA"
     gene            389..841
                     /locus_tag="VFN42_06580"
     CDS             389..841
                     /locus_tag="VFN42_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET8996316.1"
                     /translation="MASYSADLTWTLAEGEDFAKGRYSRGHRVEFDGGATLPASASPH
                     VVGKWAVPAAVDPEEMLVASLSSCHMLSFLHVARLAGFLATAYRDHAEGVMEEIAPGR
                     LAVTKVVLHPRIDWSGAQPDAAKLAQMHHEAHEACFIANSVKTQVTVA"
     gene            complement(1009..1722)
                     /locus_tag="VFN42_06585"
     CDS             complement(1009..1722)
                     /locus_tag="VFN42_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996317.1"
                     /translation="MHPTPSTAEPANRPEGDGGSVPEGIARLLQVLQWLVGCGRTFAE
                     TLHQRTAEEFRSFAGRYRRSDFNEILARMKRGLMLAAALEEKLLKRARTGHDVIPVEY
                     RYSTPRSSSAPDTTGKRTRTRHTNIVDMPPDRLPTAEQIAAELRRRPLGAVLADICRD
                     LGILPGDLTKQQWKELHELILTYGGDPTDVLFAEWHLRLLPLLEAELGKTAPAAPNTG
                     TKCHDPACPTGPPDLAKAA"
BASE COUNT          352 a          628 c          803 g          365 t
ORIGIN      
        1 ggcggagacg gcggaatcca gccgggtgcg gtagccgacg gtgcggccga ccggctcgcc
       61 gagcagcgcg gccatgcggg tggcggcagc gcgggtggcg aggcggcggg gttccagcat
      121 gacgatgcgg ccaccggcca cccagggcgc atcgcgcaac gcgagcggca ccagcgtggt
      181 cttgcccgcg ccgggcggcg cgaccagcac ggcgttgttg ccggcggcca gcgtggcggc
      241 gaggcggggc agggcgtcgg taacgggaag gtcgggaaag ggcggcatga gtggttctat
      301 atggccgcga gcaggtggtc tgcgaggcgg cttgtcgcgc cggcgaccgg tgtgcatgga
      361 tgctcgcgcc ataacgggcg gaggcagcat ggccagctac agcgcggacc tgacctggac
      421 actcgccgag ggcgaggact tcgccaaagg ccgctacagc cggggccatc gggtggagtt
      481 cgacggcggc gccacgctgc cggcttccgc atcgccgcat gtggtgggga agtgggcggt
      541 gcccgcggcg gtcgatccgg aggagatgct ggtggcgtcg ctgtccagct gccacatgct
      601 gtcgttcctg cacgtcgcgc gactggccgg gttcctggcg acggcgtacc gcgaccacgc
      661 ggaaggcgtg atggaggaga tcgcgcccgg gcggctcgcg gtgaccaagg tggtgctgca
      721 tccgcggatc gactggtccg gcgcgcagcc ggacgcggcg aagctggcgc agatgcatca
      781 cgaggcgcac gaagcctgct tcatcgccaa ctcggtgaag acgcaggtga cggtggcgta
      841 ggacatgcgg gtggcggtgc atcgggaccc ggtctttccg gggtaactgg ctgtcgcacc
      901 cgatgcgggg ggcgccgcac gccggcatga tcccgccgac aacgcccggc acgaatccgg
      961 acggcggggc aagccgtcgc cgaccccgct gcctgttgca tcgctgcgtc acgcggcctt
     1021 tgccagatcg ggcgggccgg tggggcaggc cggatcgtgg catttcgtgc cggtatttgg
     1081 cgcggcaggc gccgtcttgc ccaactcggc ttcgagcagc ggcaggagcc gcagatgcca
     1141 ttcggcgaac agcacgtccg tggggtcccc accgtaggtg agaatgagct cgtgcagttc
     1201 cttccactgc tgcttggtca gatcacccgg gaggatgccg aggtcgcggc agatatcggc
     1261 gagcacggcg ccgagcgggc ggcggcgcag ctccgcggcg atttgctccg cggtgggcag
     1321 ccggtccggc ggcatgtcca cgatgttggt atggcgggtt cgcgtgcgct tgccggtggt
     1381 gtcgggcgcg ctgctggagc gcggggtgga atagcggtat tccaccggta tgacgtcatg
     1441 gccggtcctg gcccgcttga ggagtttttc ctccagcgcc gcggcgagca tcaggccgcg
     1501 cttcatgcgg gcgaggatct cgttgaagtc gctgcgccgg tagcgtccgg cgaagctgcg
     1561 aaattcctcg gcggtgcgct ggtgcagggt ttcggcgaag gtccggccgc aaccgacaag
     1621 ccattgcagc acctgaagca ggcgggcgat cccctccggc acgctgccgc cgtccccttc
     1681 ggggcggttg gcaggctcgg cggtggaggg ggtggggtgc atgaacgtaa taggaacaca
     1741 tccgggggag gatgtcaagg ggcgttctgg aaaatgccca ggggcgatgc gcgaggctgg
     1801 atcgggcatg cgcgatcccc agggaaatat acttgagtct ctcaacggcc cctggttccg
     1861 atagcctcac aatcggggcg aactgtaatg tgcgttcttc gagagcttca cagtatcatt
     1921 tcacatccgt gctttgctcg ccccttcgtg tgcgatggct tgccaaactc atgtgatgtc
     1981 attgtcattg gggagaaccc cgctacggag ataccaaaat attatatgcg acattttagc
     2041 cgagagagtt acgaccacat cgatggtgtg gttgaggaaa tactcagaaa taaaaatctg
     2101 gatacatcca gaatatatac gaagagtgta catttagtaa cgtctctg
//
LOCUS       DASSZH010000306         2977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_757091, whole genome shotgun sequence.
ACCESSION   DASSZH010000306 DASSZH010000000
VERSION     DASSZH010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757091"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VFN42_06590"
     CDS             <1..355
                     /locus_tag="VFN42_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HET8996318.1"
                     /translation="LLESMLKVSSRLLGKAPLDVLLQGIPAGFLIASVAWIRAGVTGG
                     HFWIVLAMTYAISLGGFTHVIAGSAETFLLLFGGKIGVEHAFIGLILPALAGNVIGGT
                     GLFALLAHAQVRQEL"
     gene            complement(366..815)
                     /locus_tag="VFN42_06595"
     CDS             complement(366..815)
                     /locus_tag="VFN42_06595"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8996319.1"
                     /translation="MARDQESGITLRPASADDVHFVARVYGRAMGPVLRDAGLNLAQQ
                     GDLLLMQWSPAEIRIIRAAGRDIGWVQVAAAPDALFIRNFCIDLSWQKLGIGAAVLRM
                     LIAEASARGDAVSLGVAKGNPARRLYERFGFRITHDDAQHDYMRRDS"
     gene            complement(868..1728)
                     /locus_tag="VFN42_06600"
     CDS             complement(868..1728)
                     /locus_tag="VFN42_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HET8996320.1"
                     /translation="MIQMPIGWGWDRLRTAAHDVGSSPDEEYWPGQVRRRAVPALRRI
                     GLTDLTQALASGLDDFKANRTDVIFLCVLYPAAGLVLWALASGYGLLPLVFPLASGFA
                     LLGPLAALGLYEMSRRRELGMETSWVDAFRVLRSPSIGAIALLGVSLVVLFVLWLMAA
                     NIIYLWTLGPEPPQSLGGFLRAVFTTADGWIMIAAGLGIGFLFAVVVLAISVVSFPLL
                     LDRDTGVDTAVSFSVHSVRANPGPFAVWGMIVASGLVLGSIPCLIGLVIVLPVLGHAT
                     WHLYRRLVRD"
     gene            1930..2910
                     /gene="nadA"
                     /locus_tag="VFN42_06605"
     CDS             1930..2910
                     /gene="nadA"
                     /locus_tag="VFN42_06605"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HET8996321.1"
                     /translation="MPATALAPTRSLYEGVRHLIPPIEWPVFAEDIEAILALKRQRNA
                     VILAHNYMTPEIFHCVADITGDSLALAREAMTVDADVIVLAGVHFMAETAKLLNPDKT
                     VLIPDTRAGCSLASSITAADVRLMRQKHPGLPVVTYVNSSAAVKAESDICCTSANARK
                     IVESLGSDSVIMLPDEYLAQNVAAQTHVKIIAWAGHCEVHERFTGAEVRALRDDHPGV
                     IILAHPECPPEVIAEADYTGSTADMSAFVGREKPGRVVLLTECSMADNVSVHHPDVDF
                     VRPCNLCPHMKRITLGNIRSALEAMRHEVTIDPAIAGRARRSVERMLAVR"
BASE COUNT          529 a         1023 c          968 g          457 t
ORIGIN      
        1 tctgctcgaa tcgatgctga aggtgtccag ccggctgctc ggcaaggcgc cgctggacgt
       61 gttgttgcaa ggcataccag ccggattcct gatcgcttcc gtcgcatgga ttcgcgccgg
      121 cgtcaccggc ggccacttct ggatcgtgct ggcgatgacc tatgccatat cgctcggcgg
      181 cttcacgcac gtgatcgccg gcagcgcgga aaccttcctc ctgctgttcg gcggcaagat
      241 cggcgtggaa cacgcgttca tcggcctcat cctgcctgct ttggccggca acgtgatcgg
      301 cggcaccggc ctgttcgcgc tgctggcaca cgcccaggtc cggcaggagt tgtaagccgg
      361 taccgctacg aatcgcgccg catgtagtcg tgctgcgcat cgtcatgcgt gatgcggaag
      421 ccgaagcgct cgtacaggcg gcgtgccggg ttgcctttcg ccacgcccag gctcaccgca
      481 tcgccgcgcg ccgatgcttc ggcgatcagc atgcgcagca cggccgcgcc gatccccaat
      541 ttctgccaac tcagatcgat gcagaagttg cggatgaaca gcgcgtccgg cgcggcggcg
      601 acctgcaccc agccgatatc gcgtccggcg gcgcggatga tacggatctc ggccggactc
      661 cattgcatca gcaacaggtc gccctgttgc gcaagattca ggccggcatc acgcagcacc
      721 ggccccatgg ctcggccata gacccgcgcc acgaaatgca cgtcatcggc gctggccggt
      781 cgcagcgtga tgccgctctc ctgatcacgg gccatgcgtc actgtgcgcg tggcctctgc
      841 gtaccggcaa gcgcgacgcc gccccggtca gtcccgcacc agccgccggt acaggtgcca
      901 ggtagcgtgg cccagcacgg gcaacacgat caccaggccg atcaggcagg ggatggagcc
      961 gagcaccaat ccgctcgcga cgatcatgcc ccagacggcg aagggtccgg gattggcccg
     1021 cacgctgtgc actgagaacg acacggccgt gtccaccccg gtatcgcggt ccagcagcag
     1081 cgggaaggac accacgctga tcgccagcac cacgacggca aacaggaacc cgatgcccag
     1141 gccggcggcg atcatgatcc agccatccgc cgtggtgaac acggcgcgca ggaagccgcc
     1201 gagcgactgc ggcggctcgg ggccgagcgt ccagagatag atgatgttcg ccgccatcag
     1261 ccacagcacg aacagcacca ccagggagac tcccagcagc gcaatcgcgc cgatggaggg
     1321 ggagcgcagg acgcggaacg cgtccaccca gcttgtctcc atgcccagtt cgcggcgccg
     1381 gctcatttcg tacaggccaa gcgccgccag cgggccgagc aaggcaaagc ccgaggcaag
     1441 cgggaagacc agcggcagca ggccgtagcc ggaggcgagt gcccacagca cgaggccggc
     1501 ggcgggatac agcacgcaca ggaagatcac gtcggtgcgg ttggccttga agtcgtccag
     1561 gccgctggcc agcgcctggg tcagatcggt caggccgatg cggcgcagcg ccgggaccgc
     1621 gcgacggcgg acctggcctg gccaatactc ctcatcaggc gatgaaccca catcatgggc
     1681 ggccgtgcgc agccggtccc agccccagcc aatcggcatc tggatcatct tggttcctcc
     1741 cgaattgggt tcgggggccg cgaggcccca tcgatgcggc ccgccgtgcc catggagatg
     1801 ggttcatgtg cggcgaattt ctcgcggaat ttcggggcgg cggcagcggc gtgcgggctt
     1861 gactttctta tgctcatgac cagtataaag accattaatg ctctacatga gcatatcaag
     1921 gaggcagcca tgcccgccac cgcactcgcc cccacccgct cgctgtacga gggtgtccgc
     1981 cacctgatcc cgccgatcga atggccggtg ttcgccgagg atatcgaggc gatcctcgcg
     2041 ctgaagcgcc agcgcaacgc ggtgatcctg gcgcacaact acatgacgcc ggagatcttc
     2101 cactgcgtcg ccgatatcac cggcgacagc ctggccctgg cgcgcgaggc aatgacagtg
     2161 gatgccgacg tgatcgtgct ggccggcgtg cacttcatgg cggagaccgc caagctgctg
     2221 aacccggaca agacggtgct gatccccgac acgcgcgccg gctgctcgct ggcgtcgtcg
     2281 atcaccgcgg cggatgtgcg gctgatgcgg cagaagcatc ccggcctgcc ggtggtgacc
     2341 tacgtgaatt cctccgccgc ggtgaaggcg gaatccgaca tctgctgcac gtccgccaat
     2401 gcccggaaga tcgtcgaatc gcttggcagc gacagcgtca tcatgctgcc cgacgaatac
     2461 ctggcgcaga acgtcgcggc gcagacccat gtgaagatca tcgcctgggc cggccattgc
     2521 gaggtgcacg agcgcttcac cggtgccgag gtgcgcgcgc tgcgcgacga ccatccgggg
     2581 gtgatcatcc tggcgcaccc cgaatgcccg cccgaggtga tcgccgaggc cgactacacc
     2641 ggctccaccg ccgacatgtc cgccttcgtc ggccgggaga agccggggcg cgtggtgctg
     2701 ctgaccgaat gctcaatggc ggataacgtg tcggtgcatc atccggatgt ggacttcgtg
     2761 cgtccgtgca atttgtgccc gcacatgaag cggatcacgc tgggcaacat ccgcagcgca
     2821 ctggaggcga tgcggcacga ggtcaccatc gaccccgcca tcgccggccg cgcccggcgt
     2881 tcggtggaac gcatgctggc ggtgcgctga ccagactcct caccgccccg cgcgcactcc
     2941 caacggaggt ccgaatgatc ccgccccttc ccgccat
//
LOCUS       DASSZH010000307         2002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_382475, whole genome shotgun sequence.
ACCESSION   DASSZH010000307 DASSZH010000000
VERSION     DASSZH010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382475"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VFN42_06610"
     CDS             complement(<1..391)
                     /locus_tag="VFN42_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003625820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HET8996322.1"
                     /translation="MLRNQATDDPAIAALRARLDALPASELRRRATFAEQDMMERGIT
                     FTVYSDAEAIDRILPFDLIPRLLRAEEWQMLEAGVTQRVAALNAFLHDVYHEGRILRD
                     GVVPAALVLNNAAYCAAMRDFDVPLGCY"
     gene            918..>2002
                     /locus_tag="VFN42_06615"
     CDS             918..>2002
                     /locus_tag="VFN42_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HET8996323.1"
                     /translation="MPLHAALTHRTSYRYDRPVRLGPQAIRLRPAPHARTPVLSYALK
                     IEPAGHFLNWQQDPHGNFLARVVFPERITHFDVTVDLLADMATINPFDFFLEPEAETW
                     PFAYDPVLTEELAPFRRVLPAGPLLSALLAEMPRRDGQGTVDMLVEINRRIQTRIAYV
                     VRMEPGVLAPEETLAHASGSCRDSAWLLVQLLRHLGFAARFVSGYLVQLTADIKPIDG
                     PAGPAADFTDLHAWAEVYLPGAGWIGLDATSGLLTGEGHIPLAASPEPASAAPISGLI
                     EECETAFDFSMAIRRVRETPRVTKPYAEPVWQDILTAGRRVDAALAHGDVRLTMGGEP
                     TFIAADDMDAPEWNTTALGPTKRAYAGR"
BASE COUNT          310 a          686 c          678 g          328 t
ORIGIN      
        1 catagcagcc cagcggcacg tcgaagtcgc gcatcgcggc acagtaggcg gcattgttca
       61 gcacaagggc tgccggcacg acgccgtcgc ggaggattct gccttcgtga tagacatcgt
      121 gcaggaaggc attcagggcc gcgacgcgtt gcgtcacgcc cgcctccagc atttgccatt
      181 cctcggcgcg cagcaagcgg gggatcaggt cgaagggcag gatgcggtcg atcgcttcgg
      241 catccgaata caccgtgaag gtgatgcccc gttccatcat gtcctgttcg gcgaaggtgg
      301 cgcgccgccg aagttcggag gccggcaggg catccaggcg cgcacgcagg gcggcgatgg
      361 ccggatcgtc ggtggcttgg ttgcgcagca tctcgcagaa caggccggcc ggcgcatacc
      421 tggcgaaaag cccgcccgcg gatgggtcag gcgtggtggc acgggactgt gagtcggtct
      481 gggactggga ttgatgcacg gcctggcctt tgtcccttcc tgtcgggcca cccggcggcc
      541 cggggccgca tatcattgcc cgatgcaatg attgcctgcc agatgtattg caacccacat
      601 gccattcgcc gcgcgaccca atggtcgcaa attcgcaaac cacccgcgga aagccgctcg
      661 ccgctgcgca gggccgaggc aggatgaggc acggggtggg tgttcaatca ccacggtccg
      721 cgcggtacag gccccgggcg ggaggcatgg attgttcttc cctctcccgg catgaccggg
      781 cgatcattgc tgccggggaa aaccctccgg ccgttccgac cctcgccggg acgagcggga
      841 tcgtgaagcc gggcctggtt cttgctggac ccgtcacttg cctgaccccc cgtcctccag
      901 aacgcccgga tagttccatg ccgcttcacg cagcactcac gcatcgcacg tcctaccgtt
      961 acgaccggcc cgtcaggctc ggcccgcagg cgatccggct gcgaccggcg ccgcatgcgc
     1021 gcacgccggt cctgtcctat gcgctgaaaa tcgagccggc ggggcatttc ctcaactggc
     1081 agcaggaccc gcacggcaat tttctcgcgc gcgtcgtgtt cccggaaagg atcacccatt
     1141 tcgacgtgac ggtcgacctg ctggccgaca tggccacgat caacccgttc gacttcttcc
     1201 tggaaccgga ggccgagacc tggccgtttg cctatgaccc ggtgctgacg gaggaacttg
     1261 cgccgttccg ccgcgtgctg ccggccggtc cgctgctctc cgccctgctg gcggagatgc
     1321 cgcggcggga cgggcagggc acggtcgaca tgctggtgga gatcaaccgc cgcatccaga
     1381 cccgcatcgc ctacgtcgtg cgcatggagc cgggggtctt ggcgccggag gagacgctgg
     1441 cgcacgccag tggatcatgc cgcgactccg cgtggctgct ggtgcagctg ttgcgccatc
     1501 ttggcttcgc ggcgcgcttc gtgtccggtt acctcgtcca gctgacggcg gacatcaagc
     1561 cgatcgacgg tccggccggt ccggcggccg acttcaccga cctgcacgcc tgggcggagg
     1621 tgtatcttcc cggcgccggc tggatcggcc tcgatgccac atcggggctg cttaccggcg
     1681 aggggcacat cccgctcgcc gccagcccgg agcccgcgtc ggccgcgccg atctcgggcc
     1741 tgatcgagga atgtgaaacc gcgtttgatt tctcgatggc gatcaggcgc gtgcgggaaa
     1801 ccccgcgcgt gaccaagccc tacgccgagc cggtctggca ggacatcctg accgccgggc
     1861 gccgggtgga tgcggcgctc gcccacggcg acgtgcggct gaccatgggc ggcgagccga
     1921 ccttcatcgc cgccgacgac atggacgcgc cggaatggaa caccacggcg ctcgggccga
     1981 ccaagcgcgc ctatgccggc cg
//
LOCUS       DASSZH010000308         2167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_655135, whole genome shotgun sequence.
ACCESSION   DASSZH010000308 DASSZH010000000
VERSION     DASSZH010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655135"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VFN42_06620"
     CDS             complement(<1..494)
                     /locus_tag="VFN42_06620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET8996324.1"
                     /translation="MTTLSDRPPAEDAAKPPIDVLRGALLDSRQRWRDLVTMAADLAF
                     ETDAAGRFVFVNPDPALGWSAGLLIGQPAELLMAERNGFNPFQPTAELRGRRAWLRRG
                     DGSAACLRFAIAPLCDAQGHITGARGVGVDMTGQDAREARIAAMLRRGEVLDHILSRI
                     GQEVL"
     gene            657..2153
                     /locus_tag="VFN42_06625"
     CDS             657..2153
                     /locus_tag="VFN42_06625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET8996325.1"
                     /translation="MPPQQDHTVPEIAPGGVARAADLFQCPACRGALVESGPGLVCGR
                     CAAGYQRIDGILDFVRGRFDTQLDPHQYDAEHGISDASSAGDLRAIRALLGHRWPESL
                     GRMVEIGCGTGGFSRAVLATGAVREAVLSDVSLDMLRICQGHLERLDLANRVPVTLAT
                     YSTAEPCFRDASFDCCIGAQVLHHVPDYAAFLHDLFRFLKPGGIAFFTEPALPFHRAL
                     ACGLADLLAEQMAADPAPSHDRQVLHNWIAIQRRDLLHQGDLAYLATLEDKHMFVPGD
                     FVAMARRIGFAAVEALPLAPDPSGTVMAAGLCFRIGVTPEGTAALVRALPRHTQRHFA
                     RLAAKDRTASYLFWLRKPDFRRGSAARHNAAPAVPQRAVVDLSNARWFLEFQPEPDAR
                     GLALRLWGWCLLNSDVKWLRVCIGGVQRQVPVWYPRADVHAAFNQDGAYAAWNSLCCG
                     VSERLVFDGIAAEAEALPISVQLELINGYMINLPVHAFRPNQPLLLER"
BASE COUNT          307 a          735 c          773 g          352 t
ORIGIN      
        1 agcacctcct gcccgatgcg ggacaggatg tggtccagca cttcgccccg gcgcagcatg
       61 gcggcgatcc gcgcttccct ggcgtcctgg ccagtcatgt cgacgccgac cccgcgtgcg
      121 ccggtgatgt ggccctgggc gtcgcagagt ggcgcgatgg cgaagcgcag gcaggcggcg
      181 ctgccgtcgc cgcgccgcag ccaggcccgc cggccgcgca gttcggcagt gggctggaac
      241 gggttgaagc cgttgcgctc cgccatcagc agttcggctg gctggccgat cagcaggccg
      301 gcggaccagc ccagcgccgg atcggggttc acgaaaacga agcgtccggc ggcatcggtc
      361 tcgaacgcca ggtcggccgc catggtgacc aggtcccgcc agcgctggcg gctgtccagc
      421 agcgccccgc gcaacacatc gatggggggc ttggcggcgt cctctgctgg cggccggtcg
      481 gacagcgtgg tcatgcaccg catccctgca tatcggcatc gcattcacgc atgcatggct
      541 taacgaagcc tttccaccgg gtgcggaagg ccggaaattt cgcgcccccg ccacttttcc
      601 ggtgatcggg gggccatgga ccgctaaacc ggcgtatcgg acgggatacg caacggttgc
      661 cgccgcagca ggaccacaca gttccggaga ttgcgccggg cggggtagcg cgggcggcgg
      721 acctgttcca atgcccggcc tgccgcggcg cgctggtgga atccggcccc ggcctcgtct
      781 gcggtcgctg cgccgccggc tatcagcgga tcgacggcat cctcgacttt gtccgcgggc
      841 ggttcgacac ccagctcgat ccgcaccagt acgacgccga acacggcatc agcgacgcga
      901 gttccgccgg cgatctccgc gccatccgcg ccctgctggg ccatcgctgg ccggaatcgc
      961 tcggtcgcat ggtggaaatc ggctgcggta ccggcgggtt ttcacgcgcg gtgctggcca
     1021 ccggcgccgt gcgggaagca gtgttgagcg atgtgtcgct ggacatgctg cggatctgcc
     1081 aggggcatct ggagcggctc gacctggcga accgcgtgcc ggtgacgctg gcgacctaca
     1141 gcacggccga accctgcttc cgcgacgcca gcttcgattg ctgcatcggg gcgcaggtgc
     1201 tgcatcacgt gccggactat gcggcgttcc tgcacgacct gttccgcttc ctgaagccgg
     1261 gcgggatcgc gtttttcacc gagcccgccc tgccattcca ccgggcgctg gcctgcgggc
     1321 tggcggacct gctggcggag cagatggcag ccgacccggc cccctcgcat gaccggcaag
     1381 tgctgcacaa ctggatcgcg atccagcggc gtgacctgct gcaccagggc gacctggcct
     1441 atctcgccac gctcgaggac aagcacatgt tcgtgccggg agatttcgtg gccatggcgc
     1501 gccgcatcgg cttcgccgcg gtcgaggcgc tgccgctggc gcccgatccc tcgggtacgg
     1561 tcatggcagc aggattgtgc ttcaggatcg gcgtaacgcc tgaaggcacg gcggcgctgg
     1621 tccgggcgct gccgcgccac acgcagcggc actttgctcg gctggcggcg aaggaccgca
     1681 ccgccagcta tctgttctgg ctccgcaagc cggacttccg gcgcggctcg gcggcgcggc
     1741 acaatgcggc gccggccgtc ccgcaacgcg ccgtggtgga cctgtcgaac gcgcgctggt
     1801 tcctggagtt ccagccggag ccggatgcgc gcggactggc actgcggtta tggggctggt
     1861 gcctgctgaa cagcgacgtc aagtggctgc gcgtctgtat tggcggcgtg cagcggcagg
     1921 tgccggtgtg gtacccgcgg gccgacgtcc acgcggcatt caaccaggac ggcgcatacg
     1981 cggcctggaa ttctctgtgc tgcggggtga gcgagcggct ggtgttcgac ggcatcgcgg
     2041 cggaggccga agcgttgccg atcagcgtgc aactggagtt gatcaacggc tacatgatca
     2101 acctgcccgt gcatgccttc cggccgaacc agccattgct gctggagcgg taggggcgtg
     2161 cgttgca
//
LOCUS       DASSZH010000309         2427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_978960, whole genome shotgun sequence.
ACCESSION   DASSZH010000309 DASSZH010000000
VERSION     DASSZH010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978960"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VFN42_06630"
     CDS             <1..517
                     /locus_tag="VFN42_06630"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET8996326.1"
                     /translation="TISLSAVGGFAGNPLSQLLNASNSLWSLGAAGGETLFEGGLRTA
                     SVAAARATYDSAVASYRQTVLTAFQQVEDALSSLRILEQQAAAEARAVQSARRAVQVA
                     LNTYRAGTAVYTAVITEQTLLLVDQQSALSVQQGRLLNSVALIQALGGGWTTHDLPAP
                     DQVKTFNPLLP"
     gene            complement(498..1298)
                     /locus_tag="VFN42_06635"
     CDS             complement(498..1298)
                     /locus_tag="VFN42_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003630561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET8996327.1"
                     /translation="MPGENTAETAQVGLLPPPDDRIRVGTAGWSLPKACHAAFAADGT
                     HLQRYAGRLAAVEINSSFYRPHKPETYARWADSVPAGFRFAVKVPRTITHERRLRDID
                     APLDRFLGEVQALGGTRGPLLVQLPPGLRFDADVAGDFFEAVRARFDGPVVCEPRHPG
                     WFTDPVDALLSRFHVARVAADPAPVPLAAVPGGWTGLVYYRLHGSPRMYYSAYTDVFL
                     DRVVADLRNAARRGAEAWCIFDNTALGEATRDALSVLERLRAVMAAAG"
     gene            complement(1303..>2427)
                     /locus_tag="VFN42_06640"
     CDS             complement(1303..>2427)
                     /locus_tag="VFN42_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HET8996328.1"
                     /translation="RPALFTALRTGFADELLKQAFRATRALRAQRRRVRGLPADASAR
                     GLGKLLVVAPDQTVARQYLDRLRAWVPRCQTDTVRLATSSEADAHQVLAGFRLNAEPS
                     VLVTVAMAYEGLDAPEVAVVAALTHIRSRPWLEQMVARATRIDPHAGDYASQRALVFH
                     PDDPLFARFRLRLETEQGTLAKKPTPKKQRSLPLWLREQLADQDEQADHGITPLESNA
                     LALRYALLKPGPDLAMMRPEHEAAQAELIDPPSVIERRLRARVGEMVATQAVEDEVAM
                     QAPRGPGLYHGYNAVLKRIMGNRSRGEMTIAELEASLGWLERHRLSDHLDLLQGDPKY
                     AWTVRKRNGWAPPVGRADGRPRGAPRVWPGQSVRSAGRDP"
BASE COUNT          366 a          925 c          771 g          365 t
ORIGIN      
        1 caccatctcg ctgtccgccg tcggcggctt cgccggcaac ccgctgtccc aattgctgaa
       61 cgccagcaac agcctgtggt cgctcggggc cgccggcggc gaaacgctgt ttgagggcgg
      121 gttgcgcacc gcctcggtcg cggcggcgcg ggccacctac gattcggccg tcgcgtccta
      181 ccgccagacc gtgctgaccg ccttccagca ggtcgaggat gcgctgtcga gcctgcgtat
      241 cctggaacaa caggccgcgg ccgaggcgcg ggcggtgcaa tcggcgcggc gtgccgtgca
      301 ggtcgccctg aacacctacc gtgccgggac ggccgtctat accgccgtca taaccgagca
      361 gacgctgctg ctggtggatc agcaatccgc cctgtcggtt cagcagggcc gcctgctgaa
      421 cagcgtcgcg ctgatccagg cgcttggcgg cggctggacc acccatgacc tgcccgcgcc
      481 ggatcaggtc aagaccttca acccgctgct gccatgaccg cccgcagccg ttccagcacg
      541 ctcagcgcgt cacgcgttgc ttcgcccagc gccgtgttgt cgaagatgca ccatgcctcc
      601 gcgccgcgtc gggccgcatt ccgcagatcc gcaacaacgc ggtcgaggaa cacgtcagta
      661 taggccgagt aatacatgcg cggcgatccg tgcagccggt aatacaccag gcccgtccat
      721 ccacccggga ccgccgcaag cggcaccggc gccgggtccg ctgcgacccg cgcgacatgg
      781 aaccgggaca gaagcgcatc gaccggatcg gtgaaccagc ccggatgccg cggctcacag
      841 acaaccggcc cgtcgaaccg cgcccgcacc gcctcgaaga agtcgcccgc cacgtccgcg
      901 tcgaagcgca ggcccggcgg cagttgcacc agcaacggac cacgcgtccc gcccagcgcc
      961 tgcacctcgc ccaggaagcg gtcgagcggc gcgtctatgt cccgcagccg gcgttcatgc
     1021 gtgattgtcc gcggcacctt caccgcgaag cgaaagccgg ccggcacgct gtccgcccag
     1081 cgcgcatagg tttccggctt atgcggccgg tagaaggatg agttgatctc gaccgccgcc
     1141 agccgccccg catagcgctg caaatgcgtc ccgtcggccg cgaacgccgc gtggcacgcc
     1201 ttgggcaggc tccacccggc ggtgccgacc ctgattcgat catccggcgg cggcagcaag
     1261 ccgacctgcg cggtttcggc cgtattctcc cccggcacga cgctacgggt cccgccccgc
     1321 cgaacggacg gactgtcccg gccatacacg cggtgcgcca cgtggccgcc cgtccgcccg
     1381 gccgaccggc ggggcccatc cgttcctttt ccgcaccgtc caggcgtatt tcggatcgcc
     1441 ctgcaacaga tcgaggtgat ccgatagccg gtgccgctcc agccatccca gcgacgcttc
     1501 cagttcggcg atggtcatct cccctcggct gcggttgccc atgatccgct tcagcaccgc
     1561 attgtagccg tggtacagcc ctggcccgcg cggcgcctgc atcgccacct cgtcctccac
     1621 cgcctgcgtg gccaccatct cccccacacg cgcccgcagc cggcgctcga tcaccgatgg
     1681 cgggtcgatc agttcggcct gtgccgcctc atgctccggc cgcatcatgg ccaggtccgg
     1741 gccgggtttc agcagcgcat agcgcagcgc cagcgcgttg ctttccagcg gcgtgatccc
     1801 atggtccgcc tgctcatcct ggtcggccaa ctgctcccgc agccataacg gcagggagcg
     1861 ttgcttcttc ggcgtgggct ttttcgccag cgttccctgc tcggtttcca ggcgcaggcg
     1921 gaagcgggcg aacagcggat cgtcgggatg gaacaccagc gcccgctgcg acgcatagtc
     1981 gccggcatgc ggatcgatcc tcgtcgcccg cgccaccatc tgctccagcc atggccgact
     2041 gcggatatgc gtcagtgctg ccaccaccgc gacctcgggc gcgtccagcc cctcatacgc
     2101 catcgccacc gtcaccagca cgctgggttc ggcattcagc cggaagccgg ccagcacctg
     2161 atgcgcatct gcctcgctgg acgtcgccag ccgcaccgtg tcggtttggc accggggcac
     2221 ccaggcgcgg agtcggtcca gatattgccg agcgaccgtc tggtccggcg cgacgacgag
     2281 aagcttgccc agcccgcgcg ccgaggcatc ggcgggcagt ccacgcaccc gccggcgctg
     2341 tgcgcgcagc gcccgggtcg cgcggaacgc ctgcttcagc aactcgtcgg caaagccggt
     2401 gcgcagcgcg gtgaacaggg cagggcg
//
LOCUS       DASSZH010000310         3529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_757201, whole genome shotgun sequence.
ACCESSION   DASSZH010000310 DASSZH010000000
VERSION     DASSZH010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757201"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="VFN42_06645"
     CDS             <1..943
                     /locus_tag="VFN42_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996329.1"
                     /translation="EAAKQSKPPSSGGTSLSGTLDDEGFLTSRDEQGYRVFLTENGTW
                     SRNKDEAAKQSKSPSSGGKVSSSSDTSLSISSDSSVSFSSDSSVPPDNEHDESGRQYD
                     DLTNVLRNPQSYSKPLDQTEDMPEKTDSVQEDSTQSQTEPLYDETPDLQPENRSDEQH
                     VSSQSQEKTGGWAKGKRSEEQERGGPTIGKGRVRGTETKGGTLQPEQTGNWTQGKPSE
                     GTGRGGPTIGKGRGGTGNQGGTQQPEQTGNWSQGKPSEGTGRGGPTIGKGRGGTTSKD
                     ESQSRPDEKPQGWTGGRQRTSTVRGSGPTIGRGRRGD"
     gene            1020..1523
                     /locus_tag="VFN42_06650"
     CDS             1020..1523
                     /locus_tag="VFN42_06650"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8996330.1"
                     /translation="MPDPLPRYHLRPIRPADAEPVAALIRQAFAALPVAPDPPMSALR
                     ITAEDVTAHLASGGGALAAFQATPVASALWSAQDGGLYLGRLAVVPAWRGLGIARALI
                     AAAEAAASELGLPRIHLSTRLVLTGNRRLFAACGFAETTQTAHPGYAEPTSVAMEKWL
                     DRRGRPV"
     gene            1585..1992
                     /locus_tag="VFN42_06655"
     CDS             1585..1992
                     /locus_tag="VFN42_06655"
                     /inference="COORDINATES: protein motif:HMM:NF037081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET8996331.1"
                     /translation="MIVARLAVDLAALACSKKPRRMPMPAKKAAAPPKKAAAAKTAAQ
                     ATVTLKQIAAELAESHEVPRKQAEAMLGDLVSVTARHLKKGDKIRLTGLGILQVRKRA
                     ARMGRNPATGEAIKIKASKKIAFRPAKELKESV"
     gene            2123..2659
                     /locus_tag="VFN42_06660"
     CDS             2123..2659
                     /locus_tag="VFN42_06660"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8996332.1"
                     /translation="MTIRARLAHMGIYVKDRDAMQRFYTEVLGLMVTDQGEGRGGMHL
                     TFMSGNADNHHQMVLVTGRPDTAGFNPIQQISFMVDTLSDLREVHRRALALGATEMRP
                     ISHGNAWSIYFKDPEGNTVEAYLDTPFHVPQPHGKPLDLAKTDDEIVRETEAACRQDP
                     GFMPRDAFVRSLGERLAH"
     gene            2717..>3529
                     /locus_tag="VFN42_06665"
     CDS             2717..>3529
                     /locus_tag="VFN42_06665"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET8996333.1"
                     /translation="MAALLTVVSDTPDPTPDELIAARPHLHMHAMDGLLLDGVPLHRI
                     AEAEGTPTWVYSAAMMRERAQALTGALGDAGLDAHLHYAVKANDRLAVLRLFARQGIG
                     ADVVSEGELRRARAAGIRPEHIVFSGVGKSERELRLALSEGIGQINVESAEELDILSA
                     LAVSLGRTARVALRVNPDVDAGTHAKISTGRAQDKFGIPYADAVDLYAYAAGCPGLRP
                     VGLALHIGSQITTLAPYRAAFARAAELVRALRAAGQSVTRLDCGGGLGIGYRD"
BASE COUNT          658 a         1225 c         1152 g          494 t
ORIGIN      
        1 cgaagcggcc aagcagtcca agccaccctc atccggcggt acgtcgcttt ccggcacact
       61 ggatgacgag ggcttcctca ccagcaggga cgaacaaggc tacagggtct ttctgacaga
      121 gaatggcacc tggtcacgca acaaggacga agcggccaag cagtccaagt caccctcatc
      181 cggcggtaaa gtgtcctcct ccagcgacac cagcctgtcc atttccagcg acagcagcgt
      241 gtccttctcc agcgacagca gcgtgccgcc tgacaacgag catgatgaat ccgggcggca
      301 atacgacgat ctcacgaacg tcctccgcaa cccgcagagt tattccaagc cgctcgacca
      361 gaccgaagac atgccggaaa agacggactc tgttcaggaa gacagcacgc agtcgcagac
      421 agaaccgctc tacgacgaga cgcccgatct gcaaccggag aaccggagcg acgaacagca
      481 cgtgtcctca cagtcgcaag agaagacggg cggctgggcc aaagggaagc ggtccgagga
      541 acaggagcgt ggcggcccga ccatcggcaa ggggcgcgtc cgcggaaccg aaaccaaggg
      601 cggcacgctg caaccggagc agacgggcaa ctggacccag gggaagcctt cggagggcac
      661 tgggcgcggt ggaccaacca tcggcaaggg gcgtggcggc accggaaacc aaggcggcac
      721 gcaacaaccc gagcagacgg gcaactggag ccaagggaag ccttcagagg gcactgggcg
      781 cggtgggcca acgatcggca aggggcgtgg cggcacgacg tcgaaggatg agtcccagtc
      841 ccgccctgac gaaaagccgc agggctggac cggcggccgc cagcgcacca gcaccgtacg
      901 tggcagcggt ccgaccatcg gacgcgggcg gcgtggtgat tgagcggcac gccagcccac
      961 acgttttcca gccgcaatag cgcctccgcc cggcatgttc tgaccagccg ccgccatcca
     1021 tgcccgaccc gttgccccgc tatcatctcc gtccgatccg gcccgccgac gcggaacccg
     1081 tcgcggcgct gatccggcag gccttcgccg cattgccggt cgcgcccgat ccgcccatgt
     1141 cggcgctgcg catcaccgca gaggacgtca ccgcccatct cgccagcggc ggcggtgcgc
     1201 ttgccgcgtt ccaggcaaca cccgtcgcat cggcactgtg gtcggcgcag gatggcggtc
     1261 tgtatctcgg ccgcctcgcc gtcgtgccag cctggcgcgg cctcggcatc gcccgggcgc
     1321 tgatcgccgc ggcggaagcc gccgccagcg agctcggcct gccgcgcatc cacctcagca
     1381 caaggctggt gctcaccggc aaccggcgcc tgttcgccgc ctgcggattc gccgagacga
     1441 cgcagactgc gcatcccggc tatgccgagc ccacctccgt tgccatggaa aaatggctgg
     1501 atcgccgggg ccggccggtc tgacgcaccg cgtggaaggc gcaccggcca cgaataattg
     1561 ctgcgccggc accaacatgc tgcgatgata gttgctcgac tcgccgttga tttggcagct
     1621 ttggcgtgca gcaagaaacc caggaggatg cctatgcctg caaagaaggc cgccgcccca
     1681 ccgaagaagg cagccgcagc gaaaacggcc gctcaggcca ccgtcacgct gaagcagatc
     1741 gccgcggaac tggcggaaag ccatgaagtc ccccgcaagc aggcggaagc catgctgggc
     1801 gacctggtca gtgtgaccgc acgccacctg aagaagggcg acaagattcg cctcaccggc
     1861 cttggcatcc tgcaggtgcg caagcgcgcc gcgcggatgg gccgcaatcc ggccactggc
     1921 gaggccatca agatcaaggc cagcaagaag atcgcgttcc gccccgccaa ggagctgaag
     1981 gagtcggtct gatcgcggcg agcggcagcc atcctcgacg gccggccagc ggcggcaatc
     2041 ctcctggccg gccggttctc cttgcatcgg ccttggcctg gcccttcact gttgcccaga
     2101 aagcaaattt gaggggacgg ccatgacgat ccgcgcacgg ctcgcccata tgggcatcta
     2161 cgtcaaagac cgcgacgcca tgcagcgctt ctacaccgag gtgctcggcc tgatggtgac
     2221 cgaccagggc gaaggccgtg gcgggatgca cctcaccttc atgagcggca atgcggacaa
     2281 tcaccatcag atggtgctgg tgaccggccg cccggacacc gcgggcttca atccgatcca
     2341 gcagatctcg ttcatggtgg acaccctgtc cgacctgcgg gaggtacacc gccgcgcgct
     2401 ggcgctgggc gccaccgaga tgcggccgat cagtcacggc aatgcctggt cgatctactt
     2461 caaggatccg gaaggcaaca cggtggaggc gtatctggac acgcccttcc acgtgccgca
     2521 gccgcacggc aagccgctcg acctggccaa gacggatgac gagatcgtcc gcgagaccga
     2581 agccgcctgc cggcaggatc ccggcttcat gccgcgggac gccttcgtgc gaagcctcgg
     2641 cgaacgcctc gcccactaac gccggcgggc gacggtcgcc cgttatcaat ccccggcgcc
     2701 tgtcgtatag aggcgcatgg ccgcacttct gactgtcgtt tccgacacgc ccgatccgac
     2761 cccggacgaa ttgatcgccg cccggccgca tctgcatatg catgcgatgg acggcctgct
     2821 gctggatggc gtcccgctgc atcgcatcgc cgaggccgag gggacgccaa cctgggtcta
     2881 ttccgccgcc atgatgcgcg aacgcgccca ggcgctgacc ggcgcactcg gcgacgcggg
     2941 cctggacgca catttgcatt acgcggtgaa ggccaatgac cggctcgccg tgctgcggct
     3001 gttcgccaga cagggcattg gcgctgacgt ggtcagtgag ggagagttgc gccgtgcgcg
     3061 tgccgccggc atcaggccgg agcacatcgt gttctccggc gtcggcaagt cggaacgcga
     3121 gctgcggctg gcattgtcgg agggtatcgg ccagatcaac gtggaaagcg ccgaggaact
     3181 ggacatcctc tcggcgctcg cggtttcgct cgggcgcacc gcgcgggttg cgctgcgggt
     3241 caacccggat gtggacgccg gcacccatgc caagatctcc accggccggg cacaggacaa
     3301 gttcggcatt ccctatgccg acgccgtgga tctctatgcc tacgccgccg gatgccccgg
     3361 cctgcgtccg gtcgggctcg cgctgcacat cggcagccag atcaccacgc tcgcccccta
     3421 ccgcgccgcc ttcgcccgcg cggcggagct ggtgcgggcg ttgcgcgccg ccggccagtc
     3481 ggtcacgcgg ctggattgcg gcggcgggct cggcatcggc tatcgcgac
//
LOCUS       DASSZH010000311         3872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_126862, whole genome shotgun sequence.
ACCESSION   DASSZH010000311 DASSZH010000000
VERSION     DASSZH010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126862"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..94)
                     /locus_tag="VFN42_06670"
     CDS             complement(<1..94)
                     /locus_tag="VFN42_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996334.1"
                     /translation="MARSSPRFVCQSCGAVFPKWAGRCETCGEWN"
     gene            257..613
                     /locus_tag="VFN42_06675"
     CDS             257..613
                     /locus_tag="VFN42_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET8996335.1"
                     /translation="MNEPAELPESELPRLDPAEMEARAAEAEAFLRSLASRHRLMILC
                     RLTQGEASVGELVRCLGLTQSNLSRHLAMLREEGLVATRREGVVIHYRIASERVRPIL
                     AELYRLFCASAEQGRA"
     gene            610..1038
                     /locus_tag="VFN42_06680"
     CDS             610..1038
                     /locus_tag="VFN42_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HET8996336.1"
                     /translation="MTHFTPVAALLGGALIGLATALLWIGNGRIAGISGIVGRGATQS
                     AKPEWPWRLAFLAGLIAAPRLYALVAGRAPHLLLALHPALLVPAGLLVGFGTRLGNGC
                     TSGHGVCGLARLSPRSIAATALFLGTALLTVFLIRHGLGA"
     gene            1040..1480
                     /locus_tag="VFN42_06685"
     CDS             1040..1480
                     /locus_tag="VFN42_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HET8996337.1"
                     /translation="MTRILVGLLAGLLFGLGLTVSHMIDPAKVIGFLNIAGDWDPSLA
                     LVLFAAVTVAFAGFALARRWAAPLCDTRFSPPGKARLDMRLIAGAVLFGIGWGMVGYC
                     PGPALASLSVGGLATLTFVAAMLTGMLLFNLLDRRLPASQQPAA"
     gene            1485..2270
                     /locus_tag="VFN42_06690"
     CDS             1485..2270
                     /locus_tag="VFN42_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET8996338.1"
                     /translation="MMHAILAVLSGALVGFSLGLIGGGGSILATPLLLYVVGVKNPHV
                     AIGTGALAVALNAFANFAGHARSGTVLWRSAALFSVIGVLGAAIGSTAGKAFDGKRLL
                     FLFAILMIVVGIMMLRRRHSTSEVAHVERLDLAGVAKIAGAALLVGALSGFFGIGGGF
                     LIVPGLIFATGMPMINAIGSSLLSVGSFGATTAVNYALSGLVAWVLAAEFIGGGVLGG
                     LLGMKLALRLAPRRAALTRIFAGVIFVVAAYMLYRNIAAIGIG"
     gene            2303..3184
                     /locus_tag="VFN42_06695"
     CDS             2303..3184
                     /locus_tag="VFN42_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET8996339.1"
                     /translation="MQAPLVKGFFDKRTFSVQYVVADPDSGQCAIIDPVLDFDPNSGA
                     TATWSADALLAHVREQGYRLQWILDTHPHADHLSAAGYLKDKTGMPTAIGERVTAVQK
                     LWQAIYNLPDSFPTDGSQWDHLFADGERFRIGTLDAKVMLSPGHTLASITYVIGDAAF
                     IHDTLFMPDYGSARADFPGGDASVLWHSIQRILALPDATRLFTGHDYMPGGREPRWES
                     SVGEQKAHNKHLRQARDETAFVRMRRELDAGLPMPKLLLHSLQVNIAGGRLPPPESNG
                     RRYLKLPLDAFPTAHWA"
     gene            3189..3866
                     /locus_tag="VFN42_06700"
     CDS             3189..3866
                     /locus_tag="VFN42_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HET8996340.1"
                     /translation="MQRISRRAAIGGAVVAVGALGTLAGRWLGRSATSARAAAEPVAR
                     MPLFSDPQDPVLGNPHGTLPMAEFFDYRCPYCRRMHKLITQVLKDNPDIRYVAKEWPV
                     FGGASVTAARVALAANWQGKFAAVNAALFTTTGGLDAKSIDAAAQAAGADMAQLRHDL
                     ASRDAELQSMLGNVAMQAASLGLQGTPGFLIGTYLVPGALSYDDLTKVVAEARTKLQP
                     ASVTKVR"
BASE COUNT          574 a         1323 c         1270 g          705 t
ORIGIN      
        1 tgttccactc gccgcaggtc tcgcagcggc cggcccattt cgggaacacg gcaccgcagg
       61 actggcagac gaaacggggg ctggacctgg ccacctgtga ttctccgcaa acgtacggcc
      121 agcatgcccg gcccgcatta cccgttgctt agcgcgggga ccggagtcgc cagcacgggg
      181 cagcaattca gcaacccgtg catgcgcgaa aaccggcttg ccctcggcgc cattcatcat
      241 acatgcctca actatgatga atgagcccgc cgaactcccc gaatccgagc ttccccggct
      301 ggacccggcc gaaatggagg cgcgcgcggc ggaggccgaa gccttcctgc gttccctcgc
      361 cagccggcat cgcctgatga tcctgtgccg cctcacccaa ggcgaagcgt cggtcggtga
      421 actggtgcgc tgcctcggtc tgacccagtc caacctgtcg cggcatctcg ccatgctgcg
      481 cgaggaaggg ctggtggcga cgcggcgcga gggcgtggtg atccactacc gcatcgcctc
      541 cgagcgggtg cggccgatcc tggcggaact ctaccggctg ttctgtgcgt cggcggagca
      601 aggccgcgca tgacccactt caccccggtg gccgccctgc tcggcggcgc cctgatcggt
      661 ctggcgaccg cgctgctgtg gatcggcaac gggcgcatcg ccgggatcag cggcatcgtc
      721 ggccgcggcg caacccaatc cgccaagccg gaatggccgt ggcgcctagc cttcctggcc
      781 ggtctgatcg ccgcgccacg gctgtacgcg ctggttgccg gccgagcgcc gcatctcctg
      841 ctggcgctgc atccggccct gctggtgccg gccggcctgc tggtcggctt cggcacccgg
      901 ctcggcaatg gctgcaccag cggccacggc gtgtgcggcc tggcccgcct gtcgccacgc
      961 tcaatcgccg ccaccgcgct gttcctcggc accgcgctgc tgaccgtctt cctcatccgt
     1021 catggcctgg gagcctgaga tgacccgcat ccttgttggc ctgctcgccg gactgctgtt
     1081 cggcctgggc ctgaccgtct cgcacatgat cgacccggcc aaagtcatcg gctttctcaa
     1141 tattgccggt gactgggatc ccagcctggc gctggtcctg ttcgcggcag tcactgtcgc
     1201 cttcgccggt ttcgccctcg cccgccgttg ggccgcgccg ctgtgcgaca cgcggttttc
     1261 cccaccgggc aaggcgcgcc tggacatgcg cctgatcgcc ggcgcggtgc tgttcggcat
     1321 cggctgggga atggtcggct actgccccgg tcccgctttg gcctcgctga gcgtcggcgg
     1381 cctggcgacg ctgaccttcg tcgccgcgat gctcaccgga atgctgctgt tcaacctgct
     1441 cgaccggcgc ctgcctgcgt cgcaacagcc agccgcttga gacgatgatg cacgcaatcc
     1501 tcgccgttct gtccggcgct ctggtcggct tctccctagg actgatcggc ggtggcgggt
     1561 cgatcctggc cacaccgctg ctgctctacg tggtcggcgt gaagaacccg cacgtcgcca
     1621 tcggcaccgg cgcactcgcg gtggcgttga acgccttcgc caatttcgcc ggacatgcac
     1681 ggtccggtac cgtgctgtgg cggagcgccg cactgttttc cgtgatcggc gtgctcggcg
     1741 ccgcgatcgg ttccaccgcc ggcaaggcgt tcgacggcaa gcgcctgctg ttcctgttcg
     1801 ccattttgat gatcgtggtc ggcatcatga tgctgcgtcg ccggcacagc accagcgagg
     1861 tggcgcatgt cgaacggctg gatctggccg gcgtggcgaa gatcgccggc gccgcgctgc
     1921 tggtcggcgc gctgtccggc ttcttcggca tcggcggcgg gttcctgatc gtgccgggcc
     1981 tgatcttcgc caccggcatg ccgatgatca acgcgatcgg ctcgtcattg ctgtctgtcg
     2041 gcagcttcgg tgccaccacc gcggtcaatt acgcgttgtc cggtctggtc gcctgggtgc
     2101 tcgcggccga gttcatcggc ggcggcgtgc tgggtggcct gctcggcatg aaacttgccc
     2161 tgcggctggc gccgcgccgc gcggcgctga cgcggatctt tgctggcgtg atcttcgttg
     2221 tcgcggccta catgctgtac cgcaacatcg cggcgatcgg catcggttga aggctcgttc
     2281 gaacggttca ggagggctca ccatgcaggc acctttggtg aagggctttt tcgacaagcg
     2341 caccttcagc gtacagtacg tggttgccga tccggatagc gggcaatgcg ccattatcga
     2401 tccggtgctg gatttcgacc cgaattccgg cgccactgcg acctggtcgg ccgatgcgct
     2461 gctggcgcat gtgcgggagc agggctatcg cctgcaatgg atcctcgaca cccatccgca
     2521 cgccgatcac ctgtccgccg ccggctatct gaaggacaag accggcatgc cgaccgcaat
     2581 cggcgaacgg gtgacagcgg tgcagaagct gtggcaggcg atctacaacc tgcctgacag
     2641 tttccctacc gacggctcgc aatgggacca cctgttcgcc gatggcgaac gtttccgtat
     2701 cggcacgctg gatgcgaagg tcatgctgtc accgggccat acgctcgcct cgatcaccta
     2761 cgtgatcggc gatgccgcct tcatccacga cacgctgttc atgccggact atggctctgc
     2821 ccgcgcggat tttcccggcg gcgatgccag cgtgttgtgg cacagcatcc agcgtatcct
     2881 ggcgctgccc gacgcgacgc ggctgttcac cggccacgat tacatgccgg gcggccgcga
     2941 gccgcgctgg gaaagcagcg tcggcgagca gaaggcgcac aacaagcatc tgcggcaggc
     3001 ccgcgatgag acagctttcg tcaggatgcg ccgagagctg gacgccgggc tgccgatgcc
     3061 gaaactgctg ctgcattcgc tacaggtgaa cattgccggc ggccggttgc caccgccgga
     3121 aagcaacggc aggcgctatc tgaagttgcc gcttgacgcg ttccccaccg cacactgggc
     3181 ctagacgcat gcaacgcata tcccgccgcg ctgctatcgg tggcgctgtt gtcgccgttg
     3241 gcgcactcgg cacgctggcc ggccggtggt tggggcggtc ggcaaccagt gcgcgtgccg
     3301 ctgccgaacc ggtcgcccgg atgccgctgt tcagcgatcc gcaagacccg gtgctcggca
     3361 atccgcatgg cacgttgccg atggccgagt tcttcgatta ccgctgcccg tactgccgcc
     3421 gcatgcacaa gctgatcacg caggtgctga aggacaaccc ggatatccgc tacgttgcga
     3481 aagagtggcc ggtcttcggt ggagcgtccg tcacagcggc ccgcgtggca ctggccgcga
     3541 actggcaggg caagttcgcc gcggtaaatg ccgcgctgtt caccacgacc ggcggcctgg
     3601 acgccaaaag catcgatgcc gcggcgcagg cagcgggcgc ggacatggcg caactgcggc
     3661 atgacctggc gtcgcgcgac gcggaactgc aaagcatgct gggaaacgtc gcaatgcagg
     3721 ccgcctcgct tgggctgcag ggtacgccag gattcctcat cggcacgtat ctggtgcctg
     3781 gagcgttgtc ctacgacgat ctgaccaagg tggtcgccga agcacggacg aaattgcaac
     3841 cggccagcgt gacaaaggtt cggtgacaag cc
//
LOCUS       DASSZH010000312         3783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_518919, whole genome shotgun sequence.
ACCESSION   DASSZH010000312 DASSZH010000000
VERSION     DASSZH010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518919"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VFN42_06705"
     CDS             <1..534
                     /locus_tag="VFN42_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET8996341.1"
                     /translation="AIWGRLIAQQPGQIAYSICDAKAFPLFMPSVYPAVVADSLAELA
                     AKLKLDPAKVEAAIRQYNLGVVPAQKFDNTQLDGCHTEGLTPNKTNWARTIEKPPFYG
                     YPLRPGITFTYLGVRVNEKAQVLMDNGKPSANLYASGEIMAGNVLGRGYLAGIGMTIG
                     TVFGRIAGQEAARHARN"
     gene            521..1633
                     /gene="tcuB"
                     /locus_tag="VFN42_06710"
     CDS             521..1633
                     /gene="tcuB"
                     /locus_tag="VFN42_06710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tricarballylate utilization 4Fe-4S protein TcuB"
                     /protein_id="HET8996342.1"
                     /translation="MRETEAVERARHEMEVCNACRYCEGFCAVFPAVELRREFTAGDL
                     SYLANLCHNCRGCYYACQYAPPHEWGINVPRTLAEVRAQSYTEYAWPPSFARAFERNG
                     TLVSLLIALGLALVLVLTMALNAPGNLYAARPVAPDTFYAVIPLWAMQAVGLITFLFS
                     LFALAMGARNYWRDAGPRDGIGFGGAARGVWDILTLRNLGGGGHGCNDNSERFSMTRR
                     WLHHGMFYGFLLCFAATCVGFVYHTFLGWAAPYPFISAPVLLGTVGGVLLAIGSIGLF
                     AMKLVDDPMPAARRLLGGDVALLLLLALAALSGLGLLGLRATGAMGVALAVHLGFILA
                     LFVALPYSKMVHGVYRSAALLRWGAERKLKPVGGGE"
     gene            complement(1884..3737)
                     /gene="ptsP"
                     /locus_tag="VFN42_06715"
     CDS             complement(1884..3737)
                     /gene="ptsP"
                     /locus_tag="VFN42_06715"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010503496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET8996343.1"
                     /translation="MNSRIDPPPPQHRRLNRAAAREQVPERVFTGIPVAPGIAIGPVF
                     GAAEPAPEITRHKIQAADIAGEGARLDAAITQSRKQLAKLRARLAVLPEDSQAEIAPL
                     LDAYIRMLGPSRLVRGTRRRIEETLLSAESAVVAESEAIATAILDQAEPGLSAEDTAS
                     LQRRAEEVREIARRLIRNLTRAPFRSFADLPDGAILVCESLRPADAALLNPARLAGVA
                     TEEGGADGHTAVMLRSLGVPAVLGVPGLAHALRPGELAVVNGTDGTVVFSPAPPRLAA
                     ARRAVTAYARERQRYDKLRRLPAETADGEVAELQANLELPIELPLIAQSGACGIGLLR
                     TEFLFMNRETVPDEDTQTESYRSIIEAMNGDPVAIRVLDWGGEKDIEALSSAGIVPEI
                     ADTNPALGMRGVRLLLRHPALLETQFAAILRAAVAGPVRVMLPMVTNVSEVRAARDIY
                     ERVARRLRRRNERIPDKLPPLGIMIETPGAALSADALALEADFFAIGTNDLTAYTLAV
                     DRAEAEVAELYDPIHPAVLRLVQFATEAALRMRMPVSVCGEMAASPRLVPLLLGLGLR
                     SFSMNAAAVPRVKQAIRSVEIDACARLARRVMEQSDPARIEEMVTEFSRGG"
BASE COUNT          564 a         1265 c         1334 g          620 t
ORIGIN      
        1 gccatctggg gcaggctgat cgcgcagcag ccgggacaga tcgcctactc gatctgcgac
       61 gccaaggcgt tcccgctgtt catgccgtcg gtgtatccgg cggtggtggc ggattcactg
      121 gcggaactgg cggcgaagct gaagctcgat ccggcgaagg tggaagccgc gatccggcag
      181 tacaacctgg gcgtggtgcc ggcacagaaa ttcgacaaca cgcagttgga tggctgccac
      241 accgagggac tgacgccgaa caagaccaac tgggcgcgca ccatcgagaa gccgcccttc
      301 tatggctatc cgctgcggcc cggcatcacc ttcacctatc tcggcgtgcg ggtgaacgag
      361 aaggcgcagg tgctgatgga taacggcaag ccgagcgcga acctctatgc gagcggcgag
      421 atcatggccg gcaacgtgct gggtcgcggc tacctggcgg gaattggcat gacgatcggc
      481 acggtattcg gccgcatcgc ggggcaggag gcggcgcgcc atgcgcgaaa ctgaagcggt
      541 cgaacgtgcc cgccatgaaa tggaagtctg caatgcctgc cgctattgtg agggcttctg
      601 cgcggtgttc cccgccgtgg agctacgccg cgagttcacc gccggcgacc tgagctacct
      661 ggcaaacctc tgccacaact gccgcggctg ctactacgcc tgccagtacg cgccaccgca
      721 cgagtggggc atcaacgtgc cgcgcacgct ggccgaagtg cgggcgcaga gctacacgga
      781 atacgcgtgg ccgccgtcct tcgcccgcgc cttcgaacgc aacggcacgc tggtgtcgct
      841 gctgatcgcg ctgggcctgg cgctggtgct ggtcctgacc atggcgctga acgcaccggg
      901 caatctgtac gcggcacggc ccgtggcccc cgatacgttc tatgcggtga tcccgttatg
      961 ggcgatgcag gcggtggggt tgatcacctt cctgttctcg ctgttcgcgc tggcgatggg
     1021 cgcacgcaat tactggcgcg acgccggccc acgcgacggc atcggctttg gtggtgcggc
     1081 gcggggagtt tgggacatcc tgacgctgcg caacctcggc ggcggcggac acgggtgcaa
     1141 cgacaattcc gagcgcttct cgatgacgcg gcgctggctg caccacggca tgttctacgg
     1201 atttctcctg tgctttgccg cgacctgtgt cgggttcgtg taccacacct tcctcgggtg
     1261 ggcggcgccg tacccgttca tctccgcgcc ggtcctgctc ggcaccgtgg gcggcgtgct
     1321 gctggcgatc ggcagcatcg ggctgttcgc gatgaagctg gtggacgacc cgatgccggc
     1381 ggcgcggcgg ttgctgggcg gcgacgtcgc gctgttgctg ttgctggctt tggccgccct
     1441 gtcagggctg ggcctgctgg gattgcgcgc aaccggtgcg atgggcgtgg cgctcgcggt
     1501 gcatctgggc ttcattctcg cgctgttcgt cgcgctgccg tacagcaaga tggtgcacgg
     1561 cgtgtaccgc tccgccgcgc tgctgcgctg gggcgcggag cggaagctga agccggtcgg
     1621 cggcggcgag tgatcggggc ttaggagttg cgggcaaatt agtgtttcca ttggcgcgat
     1681 cgcgacacac gcgtcatggc tgagcctggc ccggctaccc acgtcttctc ttgttgtcag
     1741 cagcaaagac gtgggtagcc ggccttcgcc ggccatgaca cgaattgcac tgatccgccc
     1801 ggtaccgctt cgttcattgg ctgggaacaa cggagacgcg ggtggccggg ccaagcgcgg
     1861 caatgacaca cctgccgaag tcctcagccg ccgcggctga actcggtcac catttcctcg
     1921 atacgcgccg gatcggactg ctccatcacc cggcgcgcca ggcgcgcgca ggcgtcgatc
     1981 tccaccgagc ggatcgcctg cttcacccga ggcacggccg cggcgttcat gctgaaactg
     2041 cgcagcccaa gccccagcaa cagcgggacc aggcgcgggc tggcggccat ctcgccacag
     2101 accgaaaccg gcatgcgcat gcgcaacgcg gcctcggtgg cgaattgcac caggcgcagc
     2161 acggcggggt ggatcgggtc gtacagttcg gccacttcgg cttccgcgcg atccaccgcc
     2221 agcgtgtagg cggtcaggtc attggtgccg atggcgaaga aatccgcctc cagcgccagc
     2281 gcatcagcgg acagcgcggc acccggtgtt tcgatcatga tgcccagcgg cggcagcttg
     2341 tccgggatgc gttcgttgcg gcggcgcagg cggcgggcga cgcgttcgta gatgtcgcgc
     2401 gcggcgcgca cctcgctgac attggtcacc atcggcagca tgacgcggac cgggcccgcc
     2461 accgcggcac gcaggatcgc ggcgaactgg gtttccagca acgcgggatg ccgcagcagc
     2521 agccgcacgc cacgcatgcc cagcgccggg ttggtatcgg cgatctccgg cacgatcccg
     2581 gcgctggaca gcgcctcgat gtccttttcg ccgccccagt ccagcacgcg gatcgcgacc
     2641 ggatcgccgt tcatcgcctc gatgatggag cggtagctct ccgtctgggt gtcctcatcg
     2701 ggcaccgtct cgcggttcat gaacaggaat tcggtgcgca gcaggccgat gccgcaggcg
     2761 ccggactgcg cgatcaacgg caattcgatc ggcagttcca gattggcctg cagctccgcc
     2821 acctcgccat cggcggtctc ggccggcagc cggcgcagct tgtcgtagcg ctggcgttcg
     2881 cgcgcataag cggtcaccgc gcggcgggcg gcggccagcc gcggcggcgc gggactgaac
     2941 accacggttc cgtcggtccc attcaccacg gccagttcgc cagggcgcaa tgcatgcgcc
     3001 aggccgggaa cgcccagcac cgcggggacg cccagggagc gcagcatcac cgcggtatgc
     3061 ccgtcggcgc ccccctcctc cgtggcgacg ccggccagcc gagcggggtt cagcagcgcg
     3121 gcgtcggccg gccgcaggga ttcgcagacc aggatcgcgc catccggcag gtcggcgaag
     3181 ctgcggaacg gggcgcgggt caggttgcgg atcagccggc gggcgatctc ccgcacttct
     3241 tccgcccgcc gctgcaggct ggcggtatcc tccgccgaca gccccggttc ggcctggtcc
     3301 aggatggccg tggcgatcgc ctcggattcg gccaccacgg cactttcggc cgagagcaga
     3361 gtctcctcga tccgccggcg cgtgccgcgg accaggcggg aggggcccag catacggatg
     3421 tacgcgtcca gcaggggggc gatctccgcc tggctgtctt ccggcagcac ggcgaggcgg
     3481 gcgcgcagtt ttgccaattg tttgcgggac tgggtgatcg ccgcgtccag ccgagcgcct
     3541 tcgccggcga tatcggcggc ctggatcttg tgacgggtga tttccggcgc cggctccgcg
     3601 gcaccgaaca ccggaccgat ggcgatcccg ggtgcgaccg gaatgccggt gaacacgcgc
     3661 tccggcacct gctcgcgcgc ggcagcgcgg ttcaggcggc ggtgctgcgg gggcggcggg
     3721 tcaatccggc tgttcatcga agccggcctc gacgagggcg gccagcgcgt caagcgcttc
     3781 ctt
//
LOCUS       DASSZH010000313         2604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1013115, whole genome shotgun sequence.
ACCESSION   DASSZH010000313 DASSZH010000000
VERSION     DASSZH010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013115"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..695
                     /locus_tag="VFN42_06720"
     CDS             96..695
                     /locus_tag="VFN42_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003083.1"
                     /note="Members of this highly hydrophobic protein
                     family,regularly are found preceded by the yybP-ykoY
                     manganese riboswitch (see RF00080). A metal cation
                     transport function is proposed; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjbE family putative metal transport protein"
                     /protein_id="HET8996344.1"
                     /translation="MDTSLLSQELSALAQVVLIDVTLAGDNAVAVGMAVAGLPPRQRR
                     PAVMLGVAGATVIRVALGAVALELLAIIGLLLAGGLLLLWVCWKMFRELRHTVATHAA
                     SDLPHKTLGEAMLQIILADVSMSLDNVLAVAGAAHGSPWVLATGLVLSVVLMGIAANQ
                     VAKILERQRWIAWVGLLIVLYVALRMIWDGAFDVAGALA"
     gene            766..1485
                     /locus_tag="VFN42_06725"
     CDS             766..1485
                     /locus_tag="VFN42_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HET8996345.1"
                     /translation="MCTVVLLIRPGDPWPVLLAANRDERLDRAWDKPAAYWPDQPGVI
                     AGRDRSAGGTWMGANRHGVVAAVLNRPGSLGPAPGKRSRGELPLLALQHATAQQAAQR
                     IAGLDAGLWRSFNLVLADAAGAVFLRGLGHGQPQSQTLSPGMHMVTAHDPDDPDSPRV
                     ARHLPRFVAAPQPTPPDWGTWPAILADRSGDAGEQLNVVPRAGFGTVCSSLLGIRADR
                     SFAWCFAAGPPHAAPFLPLAG"
     gene            1569..2534
                     /gene="speB"
                     /locus_tag="VFN42_06730"
     CDS             1569..2534
                     /gene="speB"
                     /locus_tag="VFN42_06730"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HET8996346.1"
                     /translation="MLKPTAKYHDLHDPQARPRYTGIPTFFRTPHTEDLATVDIGVIG
                     LPFDAGVTNRSGTRHGPRAVRDQSSLLRRYSSATGARPFEMARVRDLGDCWIEQPYEL
                     TGALEEIAAFYRKVLAADVVPLSVGGDHSVSFPILRAVGERRPVGMVHIDAHCDTGDD
                     YMGSRFHHGAPFRRAVEEGVLDPQRVIQIGIRGTVNDPDQWGYSLRSGMRVLSMDEFH
                     DQGWRFAVAEARRVIGDGPAYLSFDIDSLDPSQAPGTGTPEAGGITALEALRLLRELR
                     GLDFVGGDLVEVSPPFDVGGITAFNGASVLFEILCLLVEARQKRG"
BASE COUNT          361 a          920 c          920 g          403 t
ORIGIN      
        1 cgtgccgcct ttgcgtgtac tagggcactg ccagtacctc cccaggccgc cgcgtggcaa
       61 gcgccggccg tccggacagc gaggaccccg cccgtatgga taccagcctg ctgagccagg
      121 agctgtcagc gctggcgcag gtcgtgctga tcgacgtgac cctggccggc gacaacgccg
      181 tggcggtggg catggcggtg gccgggctgc cgccgcggca gcgccggccg gcggtgatgc
      241 tgggcgtcgc cggcgccacg gtgatccgcg tggcactggg cgcggtcgcg ctggaattgc
      301 tcgccatcat tggcctgctg ctggccggcg gcctgctgct gctgtgggtc tgctggaaga
      361 tgttccgcga actgcgccac accgtcgcca cccacgccgc gtccgacctg ccgcacaaga
      421 cccttggcga ggccatgctg cagatcatcc tggccgacgt gtcgatgagc ctggacaacg
      481 tgctggccgt ggcgggcgcg gcgcatggca gcccctgggt gctggcgacc ggcctggtgc
      541 tgtcggtggt gctgatgggc atcgccgcca accaggtggc gaaaatcctg gaacggcagc
      601 gctggatcgc ctgggtcggg ctgttgatcg tgctgtatgt cgccctgcgg atgatctggg
      661 acggcgcctt cgacgtagcc ggagcgctgg cctgatccgg ccggcttgcc gcccggcccg
      721 cgcgaggcga caacacgcca tgcgcccgat ccccctgccc ttcccgtgtg caccgtcgtc
      781 ctgctgatcc gccccggcga tccctggccc gtcctgctcg ccgccaaccg cgatgagcgg
      841 ctggaccgcg cctgggacaa gccggcggca tattggcccg accagccggg agtgatcgcc
      901 ggccgcgacc gttccgccgg cggcacctgg atgggggcca accggcacgg cgtggtcgcg
      961 gcggtgctga accgccccgg cagcctggga ccggcgcccg gcaagcggtc ccgcggcgaa
     1021 ctgccgctgc tggcgctgca gcacgcaacc gcgcagcaag cggcgcagcg catcgccggc
     1081 ctggacgccg gtctctggcg cagcttcaac ctggtgctgg cggatgccgc cggtgccgta
     1141 ttcctgcgcg ggcttggcca cggccagccg cagtcacaga cgctgtcgcc cggcatgcac
     1201 atggtgaccg cacacgaccc cgacgatccg gacagcccgc gcgtcgcccg ccatctgccg
     1261 cgcttcgtcg cggcgccgca gcccaccccg cctgattggg gcacatggcc ggcgatcctg
     1321 gcggaccgca gcggcgatgc gggcgagcag ctcaacgtcg tgccgcgcgc cggcttcggc
     1381 accgtctgtt cctcgctgct gggtatccgt gcggatcgca gcttcgcgtg gtgctttgcc
     1441 gccggcccgc cgcacgcggc gcccttcctg cccttggccg ggtaaccggt accgtcaccg
     1501 aatcaagact tggcagcgcc acccgcaaag gccgatggtc tccccctgcc ggagaaggga
     1561 ggcgtacgat gctgaagccg actgcgaaat atcacgatct gcacgatccc caggcgcggc
     1621 cgcgctacac cggcattccc acgttcttcc gcacgccgca caccgaggac ctggccacgg
     1681 tggatatcgg cgtgatcggc ctgccgttcg acgccggagt gaccaaccgc agcggcacgc
     1741 ggcacggtcc gcgcgcggtg cgcgaccagt ccagcctgct gcgccgctac agcagcgcca
     1801 ccggtgccag gccattcgag atggcgcggg tgcgcgacct gggcgattgc tggatcgaac
     1861 agccctatga actgaccggc gcgctggagg agatcgccgc cttctaccgc aaggtgttgg
     1921 cggccgacgt ggtgccgctg tcggtcggcg gcgaccattc ggtcagcttt ccgatcctgc
     1981 gcgcggtggg cgaacgccgt ccggtcggca tggtgcatat cgacgcgcat tgcgacaccg
     2041 gcgacgacta catgggctcg cgcttccacc acggcgcgcc attccgccgc gctgtggagg
     2101 aaggcgtgct ggatccgcag cgggtgatcc agatcggcat ccgcggcacg gtgaacgacc
     2161 cggaccaatg gggctacagc ctgcgcagcg gcatgcgcgt gctgtcgatg gacgagttcc
     2221 atgaccaggg ctggcgcttt gccgtggccg aagcgcgccg cgtgatcggt gacgggccgg
     2281 cctatctgag cttcgacatc gacagcctgg atccgtcaca ggcgccggga accggcacgc
     2341 cggaagccgg cggcatcacc gcgctggaag cgctgcggtt gctgcgggag ttgcgcgggc
     2401 tggatttcgt cggcggcgac ctggtggaag tatcaccgcc cttcgatgtc ggcggcatca
     2461 ccgcattcaa cggggcgtcg gtgctgttcg agatcctgtg cctgctggtg gaggcacgtc
     2521 agaagcgggg ctagcactta ctccccctcc ccttgcggga gggggaggag tgtgtacctc
     2581 acccaatccg aagcaggcgc gaac
//
LOCUS       DASSZH010000314         2055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1030199, whole genome shotgun sequence.
ACCESSION   DASSZH010000314 DASSZH010000000
VERSION     DASSZH010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030199"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..948)
                     /locus_tag="VFN42_06735"
     CDS             complement(73..948)
                     /locus_tag="VFN42_06735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET8996347.1"
                     /translation="MQADMECQLEALAERYYAVGDGTRKASTYLREYEALLRQRRTAP
                     LRLLELGVSSGASVLIWRDYLPNATIVGLDLAAAPSSIRDQPRIHFIQGSQDDPEILD
                     RAGEIAGGRFDLIIDDASHLGYLTKRSFQYLFPRWLVPGGVYAIEDYGTGFMPEYPDG
                     AAFVEPPQGDSVPETQVFASHQYGMVGVLKQLMDHLMTELMTGQPPRYDIERMTILSN
                     IALVQKAGGVPMQGAASVPAPGAGADRALDQVPLMAAEMRRQGERIAALDSAMRELVE
                     QLRRNAERSILPWRR"
     gene            complement(1045..1830)
                     /locus_tag="VFN42_06740"
     CDS             complement(1045..1830)
                     /locus_tag="VFN42_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011252608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET8996348.1"
                     /translation="MLSYDFMRHAFAAAAIVAVVAGIVGYFLVLRGQTFAGHALAHVG
                     FTGATGAVLVGVAPLWGLVAVTVAAGIGMGLMGEKLAQRDVAVGLVLAFSLGLGLLFL
                     HYYTAFATQAATLLFGNVLAVDTGTVWTLAALGAVSLGMLATISRPLLFASLQPELAE
                     AKGVSLRLYAMLFLVVVALTTAACAQIVGVLLVFALMVGPAAAAQRLTATLAGGVLLS
                     AVLALAEAWGGLVLAFYTDWPSSFCIVLLSALAYVMATLPRRP"
BASE COUNT          345 a          725 c          702 g          283 t
ORIGIN      
        1 aagccgctcg gccgccggga cgctagaccg gtttgagtgc cgcagccaat tccctgcgtt
       61 gcggcttcag gcttatcggc gccagggcaa gatcgaacgc tccgcgttgc ggcgaagctg
      121 ctccaccagt tcccgcatgg cgctgtccag cgcggcgatc cgctcgccct ggcggcgcat
      181 ttcggcggcc atcagcggca cctggtccag cgcgcggtcc gctccggcgc ccggcgccgg
      241 aaccgaggcc gcgccctgca tcggcacgcc gccggccttc tgcaccaggg cgatgttgct
      301 gaggatcgtc atgcgctcga tgtcgtagcg gggcggctgg ccggtcatca gctcggtcat
      361 caaatgatcc atgagctgct tcagcacgcc gaccatgccg tactggtggc tggcgaagac
      421 ctgcgtctcc ggcacgctgt cgccctgcgg cggttcgacg aaagcggcgc cgtcggggta
      481 ttccggcatg aagccggtgc cgtaatcctc gatcgcgtac acgccgcccg gcacaagcca
      541 gcgcgggaac aggtactgga acgaccgctt ggtcaggtag ccgaggtggg aggcgtcgtc
      601 gatgatcagg tcgaagcgtc cgcccgcaat ttcgccggca cgatccagaa tttccggatc
      661 gtcctggctg ccctggatga aatggatccg cggctggtcg cggatcgatg acggcgccgc
      721 cgcaaggtcc agacccacga ttgtcgcgtt cggcagataa tcgcgccaga tcagcaccga
      781 ggcaccggac gacacgccaa gctccagcag ccgcaacggc gcggtgcggc gctggcgcag
      841 cagcgcctca tactcgcgca gataggtgga tgctttgcgg gtgccgtcgc cgaccgcgta
      901 atagcgttcg gccagcgctt cgagttggca ctccatgtcg gcctgcatcg cgactcccca
      961 tagctcgccc ggagcgccgc cgcggccccg ccagggccgc agtcatggcg ggcgcggcag
     1021 gaaaagcaag gcccggctgc cgcttcaggg gcgtctgggc agcgtcgcca tcacataggc
     1081 cagcgcgctc aacaggacga tgcagaagct gctcggccag tcggtgtaga aggccaggac
     1141 caggccgccc cacgcttccg ccagcgccag caccgccgac agcagcacgc cgccggcaag
     1201 cgtcgcggtc agccgctgcg ccgccgccgc cgggccgacc atcagcgcga agaccagcag
     1261 cacgccgacg atctgcgcgc atgcggccgt ggtcagcgcg accacgacca ggaacagcat
     1321 tgcatacagg cgcagcgaca cgcccttggc ttccgccagc tccggctgca ggctggcgaa
     1381 cagcagcggg cgggagatgg tggccagcat gcccaggctg accgcgccca gcgcggccag
     1441 cgtccatacc gtgccggtgt ccacggccag cacattgccg aacagcagcg ttgccgcctg
     1501 ggtggcgaaa gcggtgtagt agtgcaggaa cagcaggccg agcccgagcg agaaggccag
     1561 gaccaggccg acggcgacat cgcgctgtgc cagcttctcg cccatcaggc ccatgccgat
     1621 tccggccgcc acggtcaccg ccaccaagcc ccagagcggc gcgaccccga ccagcacggc
     1681 gccggtggcg ccggtgaagc cgacatgcgc cagcgcatgg ccggcgaaag tctggccgcg
     1741 cagcaccagg aaatagccga cgatgccggc caccacggcc acgatcgccg ccgccgcgaa
     1801 ggcatgccgc atgaagtcgt aggagagcat gctcaaaccg ctgcccctcc ccttgcggga
     1861 gggggatggg gggaggggag gctggaggtt gccgggggaa cgcgtgacgg ccccctcccc
     1921 ccagccccct cccgcaaggg gagggggagt gttccgtcgg ttccgctaat catgcgcatg
     1981 cccgtgcgcc ccgtgttcgt gccggtgttc gtcgtgttcc atgtcgtggc tgccggacat
     2041 cacgaagatg cggcc
//
LOCUS       DASSZH010000315         3401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1303082, whole genome shotgun sequence.
ACCESSION   DASSZH010000315 DASSZH010000000
VERSION     DASSZH010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303082"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1392)
                     /locus_tag="VFN42_06745"
     CDS             complement(<1..1392)
                     /locus_tag="VFN42_06745"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HET8996349.1"
                     /translation="MANQVELHVTDRRPFADGQAFGPSGPYERLTGRVHFAVDPLAPA
                     QRDIVDLDKAPRDADGLVRFAADCMILKPVDMARGNRRLFYDYGNRGHKRALQFFNDA
                     VHSNDPLSAAHAGNGFFMRRGYAVVWVAWEGDMLPGGGRMLLDAPVARNADGSAITGQ
                     IRVEYLADAPGVTCFPLSGRIAAYSFPTASRDTRKATLTRRRYPYDPPEVIPPDQWQF
                     ASVEIGTGGETQTPERAVVPSDRHIHLPAGFQPGWIYELLYTARDPKVMGLGHLVVRD
                     AVSYFKHDQTDANPLLGTEKAYAWGRSQTGRCLRDFVYRGFNADAQGRRVFDGVMPHV
                     AGAGRKWLNHRFASPIVSGGQQYEDHFNIADSFPFSYAWSRDHLTGREDAILKRPATD
                     PLVIHTQTCTEYWVRRGSLVHTDTQGNDLPQPDNARVYLWASSQHFADPQPKPPSRGI
                     AQNLSNVVSTSMLF"
     gene            1520..2281
                     /locus_tag="VFN42_06750"
     CDS             1520..2281
                     /locus_tag="VFN42_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996350.1"
                     /translation="MNDAPTLAERADALEARMSRDLDLIVIKSRLHSLAEGDMGPPPD
                     THALMAANPNARVLMGDDPRLPKMPEKPTLIDFFKLRFGPSMHLLQSAKHAVNAGLPE
                     KMVLACLLHDVSVIGFIRGDHGYWGAQLVEPYVDEEVTWAIRAHQVLRFYPDESVGYE
                     YPKAYIKAFGAEYRPDPYIEADYQRMRNHKWYMSGRMITVHDIYSFDPNMHVELEEFT
                     DIIGRNFRQPEQGLGFDNSPVAHMWRAMIRPAKYL"
     gene            complement(2299..2685)
                     /locus_tag="VFN42_06755"
     CDS             complement(2299..2685)
                     /locus_tag="VFN42_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996351.1"
                     /translation="MRWWRRRQDTNTREPASGSPPTIEGPYARQRRLEAIIVRRLGWV
                     LLTGLALHMTWLFRLAARSPRAPMPLLGLTAPLHIYGKWRWEATTTFFVQPWQAIVAN
                     CLLAVWLGAAFAAMVLIARAGFPRRR"
     gene            complement(2787..3041)
                     /locus_tag="VFN42_06760"
     CDS             complement(2787..3041)
                     /locus_tag="VFN42_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1150 family protein"
                     /protein_id="HET8996352.1"
                     /translation="MNTNSTTEPEAAPVQGTFDIRHLSVEQLAQLGMSQLAYVKPIVV
                     NGAPGFAIHAADGTPMAVTADRDVAIAAIMQHEMMPALVH"
     gene            complement(3143..>3401)
                     /locus_tag="VFN42_06765"
     CDS             complement(3143..>3401)
                     /locus_tag="VFN42_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HET8996353.1"
                     /translation="RQSDDGQGRIFLHRGIAARQFQRAFVLAEGIEVKGAWLDNGLLH
                     VDLARPQPESRVKTIAISRPPGAKPNGNGTGKLVEGESGRD"
BASE COUNT          577 a         1165 c         1101 g          558 t
ORIGIN      
        1 gaacagcatc gacgtggata cgacgttcga caggttctgc gcgatgcccc gcgacggcgg
       61 ctttggctgc ggatcggcaa aatgctgcga gctggcccac agatacaccc gcgcgttgtc
      121 cggctgcggc aggtcgttgc cctgcgtgtc agtgtgcacg agggagccgc gccgcaccca
      181 gtattccgtg caggtctgcg tgtggatcac cagcgggtcg gttgccggcc gcttcaggat
      241 cgcatcctcg cgcccggtca gatggtcgcg cgaccaggca taggagaacg ggaaactgtc
      301 ggcgatgttg aagtggtctt catattgctg gccgccggac acgatcgggc tggcgaagcg
      361 atggttcagc catttgcgcc ccgcgccggc gacatgcggc atgacgccgt cgaacacgcg
      421 gcggccctgc gcgtcggcat tgaagccgcg atacacgaag tcgcgcaggc aacgcccggt
      481 ctgtgaccgg ccccaggcat aggccttctc ggtgccgagc agcgggttgg cgtccgtctg
      541 gtcatgcttg aagtagctga ccgcatcgcg caccaccagg tgcccgagcc ccatcacctt
      601 cggatcgcgc gcggtgtaca gcagttcgta gatccagccc ggctggaaac ccgcgggcag
      661 atggatgtgc cggtcggacg gcaccaccgc gcgctccggc gtctgggttt cgccgccggt
      721 gccgatctcc acgctggcga actgccactg atccggcgga atcacctccg gcggatcgta
      781 gggatagcgc cgccgcgtca gcgtcgcctt gcgcgtgtcg cgcgaggcgg tcgggaaact
      841 atacgcggcg atccggccgc tgagcggaaa gcaggtcacg ccgggggcgt ccgccagata
      901 ctccacgcgg atctgcccgg tgatggcgga gccatccgca ttgcgcgcca ccggcgcgtc
      961 cagcagcatg cgtccgccgc ccggcagcat gtcgccttcc catgccaccc acaccacggc
     1021 atagccccgc cgcatgaaga agccgttgcc ggcatgggca gcgctcagcg gatcgttgct
     1081 gtgcaccgcg tcgttgaaga actgcagcgc gcgtttgtgg ccgcgattgc cgtagtcata
     1141 gaacagccgg cgattgcccc gcgccatgtc caccggcttc aggatcatgc aatccgcggc
     1201 aaaccgcacc agcccgtccg catcgcgcgg cgccttgtcc aggtccacga tgtcgcgctg
     1261 cgccggcgcc agcgggtcga ccgcgaagtg cacgcgcccg gttagccgct cgtacggacc
     1321 actggggccg aatgcctgcc cgtccgcgaa ggggcggcga tccgttacgt gcagttccac
     1381 ctggttcgcc atcgcgtcac tccaccccgt gttccggcgg agtgtgcccg cctgccggcg
     1441 ccgcctcaac ccgccaggct tgcctttccc catctgccgc gctgctatcg ccgcatgccc
     1501 cgaaggcagg gaggaagcca tgaacgacgc cccgacgctg gcggaacgcg ccgacgcctt
     1561 ggaagcacgg atgagccgcg atttggacct gatcgtcatc aagtcgcggc tgcacagcct
     1621 ggcggaaggc gacatgggcc cgccgcccga cacccacgcg ctgatggcgg cgaaccccaa
     1681 cgcgcgcgtg ctgatgggcg acgacccgcg cctgccgaag atgccggaga agccgacgct
     1741 gatcgacttc ttcaagcttc gcttcggccc gtcgatgcac ctgctgcaaa gcgcgaagca
     1801 cgcggtgaat gccgggctgc cggagaagat ggtgctggcc tgcctgctgc acgacgtctc
     1861 ggtgatcggc ttcatccgcg gcgatcacgg gtattggggc gcgcagctgg tggaacccta
     1921 tgtcgatgag gaagtcacct gggcgatccg cgcccaccag gtgctgcggt tctatccgga
     1981 cgaatcggtc ggctacgaat acccgaaggc ctatatcaag gccttcggcg ccgagtaccg
     2041 ccccgacccg tatatcgagg cggattacca gcggatgcgg aaccacaaat ggtacatgtc
     2101 gggccggatg atcaccgtgc acgacatcta ttcgttcgat ccgaacatgc acgtggagct
     2161 ggaggaattc accgacatca tcggccgcaa tttccgccag ccggaacagg ggctgggctt
     2221 cgacaacagc ccggttgcgc atatgtggcg ggcgatgatc cggccggcga agtatctgta
     2281 gcgagcggag cggttcggct agcgccggcg cggaaacccg gcgcgcgcga taaggaccat
     2341 cgccgcaaac gcggcgccga gccagactgc cagcaggcaa tttgccacaa ttgcctgcca
     2401 cggctgcacg aagaacgtgg tcgtggcctc ccagcgccac ttaccgtaaa tgtgcagtgg
     2461 cgctgtcaaa cccagaagcg gcatgggagc gcgcggcgaa cgcgctgcca agcggaacag
     2521 ccaggtcatg tgcagcgcca gaccggtgag cagcacccag ccaaggcgcc ggacgataat
     2581 cgcctccaga cgccgctggc gggcatatgg gccttcgatg gtcggcggcg acccggacgc
     2641 cggttctctg gtattggtat cctgccgccg ccgccaccac cgcatgctcg accgcctgtc
     2701 agaagcggag cgcctggtat ccgaaccggc gccaccagcc aatcagcctc cagtgcggct
     2761 tacggcaagg tgtctgccag aaagcctcag tgcaccagtg ccggcatcat ctcgtgctgc
     2821 atgatcgcgg caatcgccac gtcgcggtcg gctgtgaccg ccatcggagt gccgtccgcc
     2881 gcatgaatgg cgaagcctgg cgcaccgttc accacgatcg gcttgacgta ggccaactgg
     2941 ctcatgccga gctgcgccaa ctgttccacg gacagatgcc ggatatcaaa tgtgccctgc
     3001 accggagcgg cctcgggctc agtggtggaa ttcgtgttca ttctctctct ccttcaggcg
     3061 gcggcctttt cctcagcgcc ggcgccgttc tggtgcctgg acgggaccgc gatgcggctc
     3121 ccgtgactga ttgcaaacgg cctcagtcgc ggccggactc cccctccacc agcttgccgg
     3181 ttccgttgcc attcggcttc gctcccggcg gccgggaaat agcgatagtc ttcacccgcg
     3241 attccggttg cgggcgcgcc aaatccacgt gcagcaggcc gttgtccagc cacgccccct
     3301 tcacctcgat cccttctgcc agcacgaaag cgcgctggaa ctgccgggcc gcgatgccgc
     3361 ggtgcaggaa gatccgcccc tggccgtcgt ccgattgccg g
//
LOCUS       DASSZH010000316         2045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_962087, whole genome shotgun sequence.
ACCESSION   DASSZH010000316 DASSZH010000000
VERSION     DASSZH010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962087"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VFN42_06770"
     CDS             complement(<1..463)
                     /locus_tag="VFN42_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8996354.1"
                     /translation="MKLLWFHLMPYTELPEDFPEKHRSVWVDIHSSLFDPKRAHLMYN
                     DFMDEMEFAAECGFDAICCNEHHSNGYGLMPSPNLIAAALSRRTRDTAICVMGNSLAL
                     YNPPTRVAEEFAMIDCISGGRLIAGFPVGTPMDTCYAYGQNPSLLRERYLEA"
     gene            complement(607..984)
                     /locus_tag="VFN42_06775"
     CDS             complement(607..984)
                     /locus_tag="VFN42_06775"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996355.1"
                     /translation="MTRWSAVLLAAPVLLVLGGCTYEETPAPANTTVVVHPPSTVVQA
                     APEMAPAPPPPPHAELVPPPPQGSTAVWQPGHWRYSGDPTNPWVWANGHYVAPPAGEN
                     TWVPGRWAQQPSGQWQWISGHWA"
     gene            complement(999..1313)
                     /locus_tag="VFN42_06780"
     CDS             complement(999..1313)
                     /locus_tag="VFN42_06780"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996356.1"
                     /translation="MSVRSLAIGLAVLVVTGAAAPAASQAATVIIAPTAPPPPRVETV
                     PPPPSTVMVWHPGHWVWNGANWAWEDGHYTRRPQATAVWVPGHWVNRHGGYVWIDGHW
                     RG"
     gene            1561..>2045
                     /locus_tag="VFN42_06785"
     CDS             1561..>2045
                     /locus_tag="VFN42_06785"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET8996357.1"
                     /translation="MAPPPISGFDQRIAAVRRFSRTYTTAIGVLHDGLHASIFSLTEA
                     RVLFEIANRPGLTARALVRDLGLDPGYLSRILRHFTQRGLVLRRVVTTDRRQQEVILT
                     EAGRAAFAPLDAASRREIGAMLAAMSEPAQAELVQAMARIETLLAPAQQLPWMLRSAA
                     PG"
BASE COUNT          354 a          685 c          671 g          335 t
ORIGIN      
        1 gcgcttccag atagcgttcg cgcagcagcg acgggttctg gccataggcg tagcaggtgt
       61 ccatcggcgt gccgaccggg aagccggcga tcagccgccc gccggagatg cagtcgatca
      121 tggcgaactc ttccgccacg cgggtcggcg gattgtacag cgccagcgaa ttgcccatga
      181 cgcagatcgc ggtgtcgcgc gtgcggcgcg acagcgcggc ggcgatcagg ttcggcgacg
      241 gcatcaatcc ataaccgttg gaatggtgct cgttgcagca gatggcgtcg aagccgcatt
      301 cggccgcgaa ctccatctcg tccatgaagt cgttgtacat caggtgtgcg cgcttcgggt
      361 cgaacagcga cgaatggatg tcgacccaca ccgagcggtg cttctcgggg aagtcctccg
      421 gcagctcggt atacggcatc aggtgaaacc agagcagctt cattggacgg ctcctgggtt
      481 ggggctcttg cccgggagta tggcagccgt ccgcgcgtgg ccgtcaagcc gcggctcagg
      541 cggcgatggg ggagattgcc gcagccgccc ctctccgccc cgtggagcgg agagggaagc
      601 ccaacctcaa gcccagtgtc cggaaatcca ctgccactgg ccgcttggct gctgcgccca
      661 gcgacccggg acccaggtgt tctcgccagc gggcggcgcg acgtaatgcc cgttcgccca
      721 aacccagggg ttggtaggat cgcccgagta acgccagtga cccggctgcc agaccgccgt
      781 cgaaccctgc ggcggcggcg gcaccagttc ggcgtggggt ggcggtggcg gcgcgggcgc
      841 catttccggc gcggcctgca ccaccgtcga ggggggatgc accaccacgg tggtgttggc
      901 cggggccggt gtctcctcgt aggtgcagcc gcccagcacc agcagcaccg gggcggccaa
      961 tagaacagcg gaccaacgtg tcatgtctgt ctcctgcctc aaccgcgcca atgcccgtcg
     1021 atccacacat agccgccgtg acggttcacc cagtgacccg gtacccaaac cgcggtcgcc
     1081 tgcgggcggc gcgtgtaatg cccgtcttcc caggcccagt tggcgccgtt ccacacccaa
     1141 tggcccggat gccagaccat cacggtcgaa ggcggcggcg gtaccgtttc aacccgcggc
     1201 gggggtggag ccgtgggagc gatgatcacc gtggcggctt gcgaggcggc gggcgcggca
     1261 gcgcctgtta ccaccagcac cgccaacccg atggccagcg aacgaacact catgccccgt
     1321 ctccctgtga aaaggttcga tgcacgcagt atcatgtcaa tgtcccactt gattttgcag
     1381 gcaatacagt ggtgatgaaa taaggccgcg gccgggccga tgaatacgaa ggcgaatcac
     1441 aagcgaatga acttgccagc ggcggggccc tactcacgcg aatggcatcg ccagcactcc
     1501 aacggttaga gcaccgggca ttctattgac ataatcaact aactggttga cgatccctgg
     1561 atggcacccc ccccaatatc cggcttcgac cagcgcatcg ccgctgtccg gcggttcagc
     1621 cggacctaca ccactgcgat cggcgtcctg cacgacgggc tgcacgcttc gatcttctcg
     1681 ctgaccgaag cgcgtgtgct gttcgaaatt gccaaccgcc ctggcctgac cgcccgcgca
     1741 ctcgtgcgcg atcttgggct cgatcccggg tatctcagcc ggattctccg gcactttacc
     1801 cagcgcggac tggtgctgcg gcgtgtcgtc acgaccgatc ggcggcagca ggaagtgatc
     1861 ctgactgaag ccggccgcgc cgccttcgcg ccgctggatg ccgcatcgcg ccgcgagatt
     1921 ggcgccatgc tcgccgccat gtcggagccg gctcaggccg aactggtgca ggcgatggcc
     1981 cggatcgaga cgctgctcgc accggcacag cagctgccct ggatgttgcg cagtgcagca
     2041 cccgg
//
LOCUS       DASSZH010000317         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_92722, whole genome shotgun sequence.
ACCESSION   DASSZH010000317 DASSZH010000000
VERSION     DASSZH010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92722"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VFN42_06790"
     CDS             <1..531
                     /locus_tag="VFN42_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007399910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HET8996358.1"
                     /translation="DVAFLAARAEAMGIGLSIAPAFGDVGGPISSTRIRRLLQDGYPE
                     RANALLGRPWTIRGTVSHGDKRGRTIGFPTANIALGRHLEPARGVYAVTVRLPDGAVH
                     KGAANIGRRPTVNAGPESRLEVNLFDFSGDLYGAELTVALHAYIRAEQKFSGLEALKT
                     QIAADVAQARRLLAAA"
     gene            631..1266
                     /locus_tag="VFN42_06795"
     CDS             631..1266
                     /locus_tag="VFN42_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HET8996359.1"
                     /translation="MPLSPPAARDALHKRAIEINGYRRADGLYDIEAHLTDTKSFSQS
                     NFDRGSIAAGEPIHDMWMRLTVDETMQVVGCEAVSDHTPYAVCPQAAPNFSRLVGLRI
                     KSGFLREANHLVGGPVGCTHLRELLQQIATTAFQTINPARVRREMQAEGVPDKPGSDK
                     TDTRISEKMGGAPKIMNTCLAYAADGPVVRRRWPHLYTGPDGTQETAAAAD"
     gene            1456..2142
                     /locus_tag="VFN42_06800"
     CDS             1456..2142
                     /locus_tag="VFN42_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET8996360.1"
                     /translation="MTRLMVAFAALALLTGAWSASPARAGAIVYNLDQISSSQIGSGT
                     LGTVALTQNGSTEVDVAVTLSPGTAFVSTGGPHNAFVFNLDLRTPYEVQITSPSDGSF
                     VLGGRNRSNTPYGRFSYAIDCPGCGPGASHANPGPLAFKVTDEHGISIGDFIANSAGF
                     LFSADVIGPAGSTGNIAGDGAITVVALAGTNPSVPEPGSLPLLAAGLAALGLVLRRRR
                     GCRSVRHGGT"
BASE COUNT          358 a          722 c          769 g          334 t
ORIGIN      
        1 gacgtggcct tcttggctgc acgggccgag gcgatgggaa tcggcctctc gatcgccccg
       61 gccttcggcg atgtcggcgg tccgatttcc tcgacgcgca tccggcggct gctgcaggac
      121 ggctatccgg agcgcgccaa cgcgctgctc ggccggccct ggaccatccg aggcacggtc
      181 agccacggcg acaagcgcgg gcgcaccatc ggcttcccga ccgcgaacat tgcgctgggc
      241 cgccacctgg agccggcgcg cggggtgtac gcggtgacgg tgcggctgcc ggacggcgcg
      301 gtgcacaagg gcgcggcgaa tatcggccgc cgcccgacgg tgaatgccgg gccggagagc
      361 cggctggagg tcaatctgtt cgacttctcc ggcgatctgt acggcgcgga actgacggtg
      421 gcgctgcacg cctatatccg tgccgagcag aagttctccg ggctggaggc gctgaagacg
      481 cagatcgcgg cggatgtggc ccaggcgcgg cggctgctgg cggcggcgtg agggctaccc
      541 gcgggtagca gccctgcgca tcaggaattg cctcggtagc gccggatgca ctatggcgga
      601 tgctgcaacg caccaagcca ggagatccgg atgccgctca gcccgcccgc ggcgcgcgac
      661 gccctgcaca agcgcgccat cgagatcaac ggctaccgcc gcgccgacgg gctgtacgat
      721 atcgaggcgc acctgacgga caccaagtcg ttcagccagt cgaatttcga ccgtggcagc
      781 atcgctgccg gcgagccgat ccacgacatg tggatgcgcc tgacggtgga cgagacgatg
      841 caggtcgtcg gctgcgaggc ggtgagcgac cacacgccct acgcggtctg cccgcaggcg
      901 gcgccgaatt tttcccggct ggtggggctg cgcatcaagt cgggcttcct gcgcgaggcg
      961 aaccatcttg tgggcggccc ggtcggctgc acgcatctgc gggaactgct gcagcagatt
     1021 gcgaccacgg cgttccagac catcaatccg gcgcgggtgc ggcgggagat gcaggccgaa
     1081 ggcgtgccgg acaagccggg cagcgacaag accgacacgc ggatcagcga gaagatgggc
     1141 ggggcgccga agatcatgaa cacctgcctc gcctatgcgg cggacggccc ggtggtgcgg
     1201 cggcgctggc cgcacctgta caccggtccg gacggcacgc aggagacggc tgcggcggcg
     1261 gattagcctg aggttccggc aggcctggcg ggggcgcggc gcgtggcccc catgtgccgc
     1321 acggcaacag gatgattatt gagacaatta tagtaattag acacgagcaa aatcgcggca
     1381 aacggcgtat gggaacgcat ctcatgcgcc agattccctg ttcaggcgca ttattccgcc
     1441 aaaaggggat cgtcgatgac tcgccttatg gttgcattcg ccgcactcgc cctgctgacc
     1501 ggcgcctggt cagccagccc ggcccgggca ggcgcgatcg tctataatct cgaccagatc
     1561 tccagcagcc agatcggcag cggcacgctg ggaaccgtgg cgctgaccca gaatggcagc
     1621 accgaggtgg acgtcgccgt gacactgagt cccggcaccg cgttcgtctc caccggcgga
     1681 ccgcacaatg ccttcgtgtt caacctcgat ctgcggacgc catacgaggt gcagatcacc
     1741 agcccctcgg atggcagttt cgttctgggc gggcggaacc ggtcgaacac accctatggg
     1801 cggttttcct acgcaatcga ttgtcccggc tgcggtccgg gcgccagcca cgccaaccca
     1861 gggccgctgg cgttcaaggt gaccgatgag cacggcatct cgattggcga tttcatcgcc
     1921 aacagcgccg gcttcctgtt ctccgcggat gtgatcggtc cggccggcag caccggtaac
     1981 atcgccggcg acggggcaat caccgtcgtg gcgctggcgg gcaccaatcc ttcggttccg
     2041 gaacctggca gcctgccgct gctcgccgct ggcttggccg ccctcggcct ggtgctgcgc
     2101 cggcggcggg gctgccggag cgtccgccat ggcggcacgt gatcagcccg aggaacgggg
     2161 tgggccggag gcctggtctc cgc
//
LOCUS       DASSZH010000318         2497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1183697, whole genome shotgun sequence.
ACCESSION   DASSZH010000318 DASSZH010000000
VERSION     DASSZH010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183697"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1587)
                     /locus_tag="VFN42_06805"
     CDS             complement(<1..1587)
                     /locus_tag="VFN42_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8996361.1"
                     /translation="MLDVTGLTVSFPSEQSRAAVVRSLDLQVNAGETVAVVGESGSGK
                     SVTALAVTRLLDFAGGRIDEGSILFRDRSGTMLDLARESQERMRRLRGPEIAMIFQEP
                     MSSLNPVLRIGDQIAEAVMLHQGLGQAAARAEARRLLEQVRIADAARLLGRYPFQLSG
                     GMRQRVMIAMALSCRPRLLIADEPTTALDVTVQAQVLRLMHALQRELGMGLLFITHDM
                     GVVAEIADRVVVMRNGERVEDGEVVPIFASPRQDYTKMLLAAVPVLGSLADQPLPRAF
                     PDAAGTLLPPQDTVKDQAGPVLEIKQLVTRFDVNQGILGRRVGRIHAVEHVSLSLRPG
                     ETLALVGESGCGKSTTGRSIIRLEQPEAGSILLDGEDVTRLDRRTEALLRRGVQYVFQ
                     DPFASLDPRLTVGFSIAEPIRTHRLAAGSAIERRVMALLEQVGLPPAYAGRYPHEMSG
                     GQRQRVAIARALASEPRVIIADEAVAALDASVRVQVVNLLMALQRRLGLAYLFISHDM
                     AVVERVSHRVAVMYLGEIVEI"
     gene            1825..>2497
                     /locus_tag="VFN42_06810"
     CDS             1825..>2497
                     /locus_tag="VFN42_06810"
                     /inference="COORDINATES: protein motif:HMM:NF042870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IIL restriction-modification enzyme MmeI"
                     /protein_id="HET8996362.1"
                     /translation="MTGEAGIEAFISRWQGREGGQERANYGMFLSELCAAIGVPAPDP
                     AAATTSENDYVLERAVKLRGPDGTASNGRIDLYKRGCFVLEAKQSRETRGKPKFVETH
                     ERQMPPPPDARPVARGRRGAGRSWDVLLQNARNQAETYAKALPADHGWPPFILVCDVG
                     HVIEVFADFSGQGKNYVQFPDRNHFRVYLEDLRQPDIVDRLRRIWTDPLSLDPARHAA
                     RVTRDI"
BASE COUNT          416 a          871 c          847 g          363 t
ORIGIN      
        1 gatctccacg atctcgccga gatacatcac ggcgacccgg tggctcacgc gctccaccac
       61 cgccatgtcg tgggagatga acagataggc gaggccgagc cgccgctgca acgccatcag
      121 caggttcacc acctgcacgc ggaccgaggc atcaagcgcc gccactgcct cgtcggcgat
      181 gatcacgcgc ggctcgctgg ccagcgcgcg ggcgatggcg acgcgctggc gctgcccgcc
      241 ggacatctcg tgcgggtagc gccccgcata ggcgggcggc aggccgacct gctccagaag
      301 cgccatgaca cgccgctcga tggccgaccc cgccgccaga cgatgggtgc ggatcggctc
      361 ggcaatcgaa aagccgacgg tcaggcgggg atcgagcgag gcgaacgggt cctggaacac
      421 atattgcacg ccgcggcgga gcagcgcctc ggtccgccgg tcgaggcggg tcacgtcctc
      481 gccgtcgagc aggatgctgc cggcctcggg ctgctccagc cggatgatgc tgcggccggt
      541 ggtggacttg ccgcagccgg attcacccac cagcgccagg gtctcgccag ggcgaagcga
      601 gaggctgaca tgctcgacgg cgtgaatgcg gccgacgcgc cggccgagga tcccctggtt
      661 cacgtcgaag cgggtgacca gttgcttgat ctccagcacc ggcccggcct ggtccttcac
      721 cgtgtcctgc ggcgggagca gagtgccggc ggcatcggga aaggcacgcg gcagcggctg
      781 atcggcaagg ctgcccagca ccggcaccgc cgccagcagc atcttcgtgt agtcctggcg
      841 gggggaggcg aaaatcggca cgacctcgcc gtcctcgacg cgctcgccgt tgcgcatcac
      901 caccacgcgg tcggcgatct ccgccaccac gcccatgtca tgggtgatga acagcaggcc
      961 catgccgagt tcgcgttgca gcgcatgcat caggcgcagc acctgcgcct gcacggtgac
     1021 gtccagcgcc gtggtgggtt cgtcggcgat cagcaggcgg ggccggcagg acaaggccat
     1081 cgcgatcatc acccgctggc gcatgccccc cgagagctgg aacgggtagc ggccgagcag
     1141 ccgcgccgcg tcggcaatcc gcacctgctc cagcaggcgg cgcgcctcgg cgcgcgcggc
     1201 ggcctgtccc agcccttgat gcagcatcac cgcctcggcg atctggtcgc cgatgcgcag
     1261 gacggggttc aggctgctca tcggctcctg gaagatcatg gcgatctcgg ggccgcgcag
     1321 gcgccgcatc cgctcctggc tctcacgcgc cagatccagc atggtgccgg accggtcgcg
     1381 gaacaggatg cttccttcgt cgatccggcc gccggcgaaa tcgagcagcc gggtgacggc
     1441 aagcgcggtc accgatttgc cggagccgga ttcgcccacc acggcgacgg tttcacccgc
     1501 gttcacctgc aaatccaggc tccgcacgac ggcggcacgg ctctgttccg atgggaagct
     1561 gacggtcagc cccgtgacgt cgagcatggg cgatgcgatg acgctatccg gcttcacgga
     1621 ccagccgcag cttcaactgc tccatccggc gtcgctgccc gagcttcttc aggataacgg
     1681 gagccgcgac agaggcagta cgaactcggc atcggggcaa actatcacgc gccggaccgt
     1741 tccgccacca tgatgcccgc cggtggacat gatctcctgt ctccgtagct ttccttcgca
     1801 aaacatttcg gtcatcttcg tgccgtgacc ggggaagcag gaatcgaggc gttcatttcg
     1861 cgctggcagg gccgcgaggg cgggcaggag cgcgccaatt acgggatgtt cctatccgag
     1921 ctgtgcgcgg ccatcggcgt gccggccccc gatccggccg ccgcgaccac cagcgagaat
     1981 gattacgtgc tggagcgcgc ggtgaagctg cgcgggccgg acggcacggc aagcaacggg
     2041 cggatcgacc tctacaagcg cggctgcttc gtgctggagg ccaagcagag ccgggagacg
     2101 cgcggcaagc cgaaattcgt cgagacgcat gagcggcaga tgccgccgcc gcccgatgcc
     2161 aggcccgtgg cacgcggccg gcgcggtgcc ggccgaagct gggacgtgct gctgcagaat
     2221 gcccggaacc aggcggaaac ctatgccaag gcactgcccg ccgatcatgg ctggccgccc
     2281 ttcatcctgg tctgcgatgt cgggcatgtc atcgaggtgt tcgccgactt ttccggccag
     2341 ggcaagaact acgtccagtt ccccgaccgc aaccacttcc gcgtctatct cgaagacctg
     2401 cgccagccgg acatcgtgga ccggctgcgc cgcatctgga ccgatccgct ctcgctcgat
     2461 ccggcgcgcc acgcggcaag ggtgacgcgc gacatcg
//
LOCUS       DASSZH010000319         2651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_24793, whole genome shotgun sequence.
ACCESSION   DASSZH010000319 DASSZH010000000
VERSION     DASSZH010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24793"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFN42_06815"
     CDS             <1..262
                     /locus_tag="VFN42_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996363.1"
                     /translation="RLPPAAELPTAIVEPTGRVRFRPRAFARAVTAAARTARARSLRA
                     RRSVARASRMAGFGAATLAVAAAASTWLAARRRRRLPVPLPR"
     gene            complement(414..1415)
                     /locus_tag="VFN42_06820"
     CDS             complement(414..1415)
                     /locus_tag="VFN42_06820"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HET8996364.1"
                     /translation="MPGNAPSAPPPDRVPNPPNPRQRRQPLPWHQPKPSAEDPAAPEA
                     VRAIIDSPTYREADRDVDFLDRNDTRGVRLQLDYLKAELLLQGRGVAHTVVVFGSTRI
                     PQPDTARRALESCQAAAARAPGDAVVQQRLAVARRAADNSRYYDVARRFGQLVGAAGE
                     QALGGRIMVMTGGGPGIMEAANRGAHDLGKHSIGLNITLPDEQYPNPYVTPELCFSFH
                     YFSMRKLHFLLRARALVVFPGGFGTMDELFELLTLVQTRKISPLPIILVGETFWRQAF
                     NPDFLVNEGVIDPEDRELFWYAETAEQIWDDILLWYEAAGRPLLAPGQTAATGKARQ"
     gene            complement(1597..2133)
                     /locus_tag="VFN42_06825"
     CDS             complement(1597..2133)
                     /locus_tag="VFN42_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996365.1"
                     /translation="MAERNRDRAAPSDADGTRDVRHAAKQEAWGSAILPPCRLALLTL
                     LFGAGVVLRSSGTAQAAYHGQPASPEIHAWFDYLKIPPIYVEGCCGYHRDCWETRAYY
                     RHGQWWALYRPFATDPKSFNKDARFIPIPPGSVESPAHLYKHYNLTGRPVLCALPHVP
                     PNFGRPSVYCFVPPPTSF"
BASE COUNT          394 a          902 c          933 g          422 t
ORIGIN      
        1 ccggctgccg ccggcggccg aactgccgac cgccatcgtc gaaccgaccg gccgggtccg
       61 cttccgcccg cgcgctttcg cccgggccgt caccgcggcg gcacggacgg cgcgcgcgcg
      121 aagcctgcgg gcgcggcgat cggtggcacg ggcatcccgg atggccgggt tcggcgccgc
      181 gacgctggcc gtggccgccg ccgccagtac atggctggcc gcacggcggc ggcgcagact
      241 gccggtgccg ctgccgcgct agcggctaat gcggcctgcc tgaggcggcg tgggcaaccg
      301 gctccggcca tcgccgggcg ccggccagcc gcattgccgg cgcttcgccg agttcgggaa
      361 aggtcagttc gcggcggctg atggcgtggt gccgggacac ccgcgcatgc gggtcattgc
      421 ctggccttcc cggttgcggc ggtctggccc ggagccagca gcggccggcc cgcggcttca
      481 taccagagca ggatgtcatc ccagatctgc tccgccgttt ccgcgtacca gaacagctcg
      541 cggtcttccg gatcgatgac gccctcgttc accaggaaat ccgggttgaa cgcctggcgc
      601 cagaaggtct cgccaaccag gatgatcggc agcgggctga tcttgcgggt ctgcaccagg
      661 gtgagcagtt caaacagctc atccatcgtg ccgaagccgc cgggaaacac caccaatgcc
      721 cgcgcgcgca acaggaagtg cagcttgcgc atggagaagt agtggaagct gaagcagagt
      781 tcgggtgtga cgtaggggtt gggatactgc tcgtccggca gggtgatgtt caggccgatc
      841 gagtgcttgc ccaggtcgtg cgcaccgcgg ttggctgcct ccatgatgcc tgggccgccg
      901 ccggtcatca ccatgatgcg cccgccgagc gcctgctcgc cggcggcgcc gaccagttgc
      961 ccgaagcggc gggcgacgtc gtaatagcgg ctgttgtcgg cggctcggcg cgcgaccgcc
     1021 agccgctgct gcaccactgc atcgcccggt gcgcgcgccg ccgccgcctg gcaggattcc
     1081 agcgcgcggc gggcggtgtc gggctgcggg atgcgcgtgc tgccgaacac gacgacggta
     1141 tgcgccacgc cacggccttg cagcagcaat tcggccttca ggtagtcgag ctgcagccgc
     1201 acgccgcggg tgtcgttcct gtccaggaag tccacatcgc ggtcggcttc gcggtacgtc
     1261 ggactgtcga tgatggccct gacggcttcc ggtgcggccg gatcttccgc ggagggtttt
     1321 ggctgatgcc acggcagcgg ctggcggcgc tgcctggggt tcggcgggtt gggaactcgg
     1381 tccggcggcg gtgcggacgg cgcgttgccg ggcatggtcc ggtccatctg tgcacacggg
     1441 aagcgggttt cccgtcgcaa tggaggttag gaaagcgggt gcccccgggc actgatccag
     1501 atcaatgccg tggccccggg acgcgcgatc ggagccaacg gtgccatcgc gtcgccggcc
     1561 ggcgggcatc atcgcgtccg tcgcggccgc gcgcttctag aacgaggtcg gcggcggcac
     1621 gaagcagtac acactcggcc tgccgaagtt cggcggtacg tgcggcagcg cgcacaagac
     1681 cggccgtccg gtcaggttgt aatgcttgta caggtgcgct gggctctcca cactgcccgg
     1741 cggtatcggg atgaaccgcg cgtccttgtt gaagctcttg ggatcggtgg cgaaggggcg
     1801 atacagcgcc caccactggc catggcgata gtacgccctg gtctcccagc aatcacgatg
     1861 gtagccgcag cacccttcga catagatcgg gggaatcttc aggtagtcga accacgcatg
     1921 gatctcgggt gatgcgggct ggccgtgata cgcggcctgg gcggtaccgg aactccgcag
     1981 gacaacgccc gcaccgaaca gcagcgtgag cagcgccagc cggcagggtg gcaggattgc
     2041 gctgccccag gcttcctgct ttgccgcgtg cctgacgtcc cgggttccgt cggcatcgga
     2101 cggggcggcc cggtcgcgat ttcgctcagc caccggcctc ctccccgatt tggcctgcgc
     2161 cgttccgtct tatcggtcgc cttatagcag cagagtatgc gccgcccggg ccagtccagc
     2221 atctcggaca gtgacctgcc agtcacgctc ctgtgcggcg cggttgatca ccgtgctggc
     2281 cgctgcacac cgcagccagg cgcgggaggc ggtcaaggca aatccggcca gcatggtgcg
     2341 gggcggagta caggcaacag gtggaacgga cctcgccgcc ggcggccgcc cgggtggcgc
     2401 gccggagccg ctgcctgggc atgctttccg gcgagtcatg gaaaaaatgc cttgacaaca
     2461 ttgccactca tgttctatag acgttccaag cgagccaacg ccccacccca acatcggagc
     2521 cccccacacc gtgcccgaac gcatcggtcg cctgctcacc ctggttcagt tcctgatcgg
     2581 ctacggcaag caatgcaccg ccacgctgcg gcagcatgcc gtcacgccgc aggtccggct
     2641 gaccctgcag g
//
LOCUS       DASSZH010000320         3446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_246529, whole genome shotgun sequence.
ACCESSION   DASSZH010000320 DASSZH010000000
VERSION     DASSZH010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246529"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1243)
                     /locus_tag="VFN42_06830"
     CDS             complement(167..1243)
                     /locus_tag="VFN42_06830"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HET8996366.1"
                     /translation="MMDGIAPLAARLQPVSGRNVWYGPEMARRQDWIRPLTAAEIAAL
                     DSAVARLDASGIAIEAIDASQMESPVLRALVDEIRHAVLHDTGFILLRGVPVERWTTR
                     QCAIAYFGLGTIIGEPVSQNAMGHILGHVKDIGFDYHRPTHRGYQTAARLPYHCDSSD
                     IVALLCLKPSRSGGLSSIVSSGALYNEMLRRDPALVAALCCPVYRDRRGEVPAGADPW
                     YAVPVFNPMPDGSLVTTYVRSSILKAQRFPEVPRVTPELDAACNLLDSLAESGEIHLD
                     MEFRPGDIQFLNNHWILHSRTAFEDFAEPERRRHLFRLWLACADGPKLPEVFTRVWQG
                     ETANGRPAGIRVPGVPLTAPLDAV"
     gene            complement(1249..2037)
                     /locus_tag="VFN42_06835"
     CDS             complement(1249..2037)
                     /locus_tag="VFN42_06835"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET8996367.1"
                     /translation="MLAGLQLRSTNHPASGAPAMPHPDGNGLVVFRWFGANPPPTLAL
                     PEQIAAMVGDRIVSGAIAPGTRIIEQDIASTFETSRGPVREALRILEREGLVRIHPRR
                     GARATQLSPQDVYDLFEIRACLYQAVARRLAEQRPPEAIRLLEDGVRDMRRLAEQTDG
                     ADLYSQLSFRLSLGCVRLAGNARLADMITSLALQTYRYTRLGLDSRTRRRNSVATWQA
                     SLTAIRDGDVATAMQMAERRIFAGREEVLRLLRAEAKASEANQR"
     gene            2162..3040
                     /locus_tag="VFN42_06840"
     CDS             2162..3040
                     /locus_tag="VFN42_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007399429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HET8996368.1"
                     /translation="MSALRGNNGPRYRHLSDDAAPFETITVDKMTPIIGAEIGGVDLA
                     QPSNQQMDEIHRALAENLVIFFRDQTMTPEQHLDFGRRFGPLHLHPAAPHVEGLPELM
                     IIHADKDSPRANGEGWHSDVSCDVEPPMGSILYIKTCPPRGGDTLFASMYAAYEALSD
                     RMKQYLEGLTAMHDGEAAYRGTYANYGVKDKQTYPRAEHPVIRTHPVTGRKALYVNRG
                     FTRRILGIPRDESDGVLAYLFSHMENPLFQCRFRWRANSVAFWDNRCVQHRAMWDYWP
                     HTRSGNRVTVKGDRPV"
     gene            3058..>3446
                     /locus_tag="VFN42_06845"
     CDS             3058..>3446
                     /locus_tag="VFN42_06845"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HET8996369.1"
                     /translation="MSYQSIDVRTLTPTIGAEIFGVDLGGGISNLQFQEIHDALLKHL
                     VIFFRDQTLTPEQHKEFGRRFGKLHIHPASNNVLADHPEVLIIKADEKSKRVAGEVWH
                     SDVSCDPEPPMGSILYMHQPPENGGGDT"
BASE COUNT          628 a         1142 c         1134 g          542 t
ORIGIN      
        1 ctgggacggc gcgcaattgc tggcgaagcc gggcgatgga cgcttcgttg cgcgtcgcgg
       61 cgggtagcgc gaccggctgc acggcgcgcc ccgcccgcat caggccgtgt tcgccgcctg
      121 cgtcagcccg acgcgcatag ccgcgccgcg ggggggccgc tagccgtcac accgcgtcga
      181 gcggcgccgt caacggcaca ccaggcacac gaatcccggc ggggcggcca ttcgcggttt
      241 cgccctgcca gacgcgcgtg aacacctccg gcagcttcgg cccgtcggcg caggccagcc
      301 acaggcgaaa caggtggcgg cgccgctccg gctcggcgaa atcctcgaac gcggtgcgcg
      361 agtgcaggat ccaatggttg ttgaggaact ggatgtcacc gggacggaat tccatgtcca
      421 ggtggatttc cccactctcg gcaaggctgt ccagcaggtt gcaggcggca tccagctccg
      481 gcgtcacgcg cggcacctcg ggaaagcgct gcgctttcag gatcgagctg cggacatagg
      541 tggtgaccag gctgccatcc ggcatcgggt tgaacaccgg cacggcgtac cagggatcgg
      601 cgcccgccgg cacctcgccg cgccgatcgc ggtacaccgg gcagcacagc gccgcgacca
      661 gcgccggatc gcggcgcagc atttcgttgt agagcgcccc ggaactgaca atgctcgaca
      721 gcccgccgga gcgtgatggc ttcaggcaca gcagcgccac gatatccgac gagtcgcagt
      781 gatagggcag ccgcgcggcg gtctgatagc cgcgatgcgt cggccgatgg tagtcgaagc
      841 caatatcctt cacgtgaccg aggatatggc ccatcgcgtt ctgcgacacc ggctcgccga
      901 tgatcgtgcc aaggccgaaa tacgcgatgg cgcactggcg cgtcgtccag cgctccaccg
      961 gcacgccgcg cagcaggatg aatccggtgt catgcagcac cgcatgccgg atctcatcga
     1021 ccagggcgcg caggaccggc gattccatct ggctggcgtc gatcgcctcg atagcgatgc
     1081 cggacgcgtc cagtcgcgcg accgccgagt ccaaagccgc gatctcggcg gcggtcagcg
     1141 gccggatcca gtcctgccgc cgcgccatct ccggaccgta ccagacattg cggccggaca
     1201 ccggctggag cctggccgcg agaggagcga tgccgtccat catgcaggtt acctttggtt
     1261 cgcttcggat gccttggctt cggcccgcaa cagccgcagc acttcctcgc gtccggcgaa
     1321 gatacgcctc tccgccatct gcatggcggt ggcaacgtcg ccgtcgcgga tcgcggtcag
     1381 cgacgcctgc cacgtcgcga cggaattgcg ccggcgggtg cgggaatcga ggccgagcct
     1441 tgtgtagcga taggtctgca gcgcgagcga ggtgatcatg tcggccaatc gcgcattgcc
     1501 cgccaaccgc acgcagccga ggctgagccg gaacgacaat tgcgaataca gatccgcccc
     1561 gtccgtctgc tcggccaggc ggcgcatgtc gcgcacgcca tcctccagca gccggatggc
     1621 ttcgggcgga cgctgctccg ccaggcgccg cgcaacggcc tgatagagac aggcacggat
     1681 ctcaaacagg tcgtacacgt cctgcggcga aagctgggtg gcgcgcgccc cgcggcgcgg
     1741 atggatgcgc accagtcctt cgcgctccag aatgcgcagc gcttcacgca ccgggccacg
     1801 cgatgtctcg aaggtggagg cgatatcctg ctcgatgatg cgggtgcccg gtgcgatggc
     1861 gccggagacg atgcggtcgc cgaccatcgc ggcaatttgt tcgggcaatg ccagggtggg
     1921 cggcggattg gcgccgaacc agcgaaacac tacgagccca ttgccgtcgg gatgcggcat
     1981 ggccggcgcg ccggatgccg gatggttggt tgaccgaagc tgcagacctg cgagcacgat
     2041 ggattcgacc ctccctgtcg cccgcatcct agcgggaccc ccgattgtcg ccaaggtaga
     2101 ttgttgacaa tccggcaatc gccggtaacc tgcgatcggg gatgttcaag agaggcatgc
     2161 aatgagcgcg ttgcgtggca acaatgggcc gcggtaccgt catctgtccg acgacgccgc
     2221 gcccttcgag acgatcaccg tggacaagat gacgccgatc atcggcgccg aaatcggcgg
     2281 cgtcgatctg gcgcagccaa gcaaccagca gatggacgag atccatcggg cgctggcaga
     2341 aaacctggtg atcttcttcc gcgatcagac aatgacgccg gagcagcatc tggatttcgg
     2401 ccgccgcttc ggcccgctgc atcttcatcc ggcggcgccg cacgtggaag gcctgccgga
     2461 gctgatgatc atccacgccg acaaggacag tccgcgcgcc aacggcgaag gttggcacag
     2521 cgacgtgtcc tgcgatgtcg agccgccgat gggcagcatc ctgtacatca agacctgccc
     2581 gccccgcggc ggcgacacgc tgtttgccag catgtatgcc gcgtatgagg cgctgtcgga
     2641 tcgcatgaag cagtacctgg aagggctgac cgcgatgcac gatggcgaag ccgcctatcg
     2701 cggcacctat gccaattatg gcgtcaagga caagcagacc tacccgcgtg ccgaacatcc
     2761 ggtgatccgc acgcatccgg tgaccggcag gaaggcgctg tatgtcaatc gcggcttcac
     2821 ccgccgcatt cttggcattc cgcgcgacga gagcgacggt gtgctggcct acctgttctc
     2881 ccacatggaa aatccgctgt tccagtgccg cttccgttgg cgggccaatt ccgtcgcgtt
     2941 ctgggacaat cgctgcgtgc agcatcgggc gatgtgggat tactggccgc acacgcggtc
     3001 cggcaatcgg gttaccgtca agggcgatcg gcccgtctaa gccaagggga aagaaacgtg
     3061 agctatcaat cgatcgacgt gcgtaccctg acaccgacaa tcggcgcgga aatcttcggc
     3121 gtcgacctgg gtggcggaat cagcaacctg cagttccagg aaatccacga cgcgctgctg
     3181 aagcacctgg tgatcttctt ccgggaccag acgctgaccc cggaacagca caaggagttc
     3241 ggccgccgct tcggcaagct gcacatccac ccggcctcca acaacgtgct ggcggaccat
     3301 cccgaagtcc tgatcatcaa ggccgacgag aagtccaagc gcgttgcggg cgaggtatgg
     3361 cattccgacg tgtcctgcga tcccgagccg cccatgggct cgatcctgta catgcaccag
     3421 ccgccggaaa atggcggtgg tgacac
//
LOCUS       DASSZH010000321         3486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1235191, whole genome shotgun sequence.
ACCESSION   DASSZH010000321 DASSZH010000000
VERSION     DASSZH010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235191"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VFN42_06850"
     CDS             complement(<1..278)
                     /locus_tag="VFN42_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8996370.1"
                     /translation="MKQTIALVDDDRNILTSVSMTLEQEGFQVRTFTDGESALQGLTA
                     KPADLAVLDIKMPRMDGMELLQRLRQRSTMPVIFLTSKDEEVDELMGLR"
     gene            complement(424..1242)
                     /gene="rlmJ"
                     /locus_tag="VFN42_06855"
     CDS             complement(424..1242)
                     /gene="rlmJ"
                     /locus_tag="VFN42_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008850739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HET8996371.1"
                     /translation="MNYRHAFHAGNFADCMKHALLLWLIEALQQKPAPIFVLDTHAGA
                     GAYDLDAAEAQRTGEWRSGIGRLLDSPPLALAPYVRLVRRLGLYPGSPALIRAVLRPD
                     DRLACCELHPEDAFELRRRFARDKQVAVHRRDGWEALGALLPPKERRGLVLIDPPFED
                     PQEFSRLAQALAAAQARFPSGVFAAWYPVKHRAPVRGFHAMLQQSGIPDVVVAELLLR
                     EPVDPTRLNGCGLAVINPPFRFELAAPGILAALLDRLCPNPQDGGTTVARLTQE"
     gene            complement(1431..2255)
                     /locus_tag="VFN42_06860"
     CDS             complement(1431..2255)
                     /locus_tag="VFN42_06860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET8996372.1"
                     /translation="MRLGIHLPHIGRKAGPDAIRRSAEQAEALGFADVWVSEHIIVPK
                     DAAYPPSPNFWDPVLTLTWAAAYTKRVRLGTSVLVLPMRHPLPLAKELATLQNLSQGR
                     LILGAGVGWMEAEFAALGVPFRERGRRMDEGIALMRAVWNDDPVSFATRWIPAEVREM
                     RMQPQPCAPIPIWIGGSSDAAIQRALRLDGWHGSRVAPEQAKPMVQRLRAARPEAAFT
                     ISLRVGCSAGTVGALHDTLAAYRDAGVQHVLVAPEDRDIDTYLATTEQVARAAAGL"
     gene            complement(2530..2802)
                     /locus_tag="VFN42_06865"
     CDS             complement(2530..2802)
                     /locus_tag="VFN42_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996373.1"
                     /translation="MHKLFTAGIALAGVFGLVPLAAAAAPLAPAPQAAVTATVQHVDW
                     DNDHCGPRCQYWRHRRWEREHAWRREQWRREHYGWNYRHEHWDEAR"
     gene            2990..>3486
                     /locus_tag="VFN42_06870"
     CDS             2990..>3486
                     /locus_tag="VFN42_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HET8996374.1"
                     /translation="MIDTTITLEDKYTRATGRVLLSGTQALVRLPLMQRARDAAAGLD
                     TRGFISGYRGSPLGNYDAALWDTGRAIHEAGIVFRPGVNEELAATACWGTQQVGLIEG
                     AKHDGVFAIWYGKGPGVSRAGDALKHGNFAGTSPHGGVLVLAGDDHAAKSSTSAHQSD
                     PELAAA"
BASE COUNT          537 a         1241 c         1209 g          499 t
ORIGIN      
        1 cgcaggccca tcaattcgtc cacctcctcg tccttggagg tgagaaagat caccggcatg
       61 gtgctgcgct ggcgcaggcg ctgcagcagc tccatgccgt ccatgcgcgg catcttgata
      121 tccagcacgg cgaggtcggc cggcttcgcg gtcaatccct gcagcgcgct ttcgccgtcg
      181 gtgaaagtac ggacctggaa gccttcctgc tccagggtca tggacacgct ggtcaggatg
      241 ttccggtcgt cgtcgaccag ggcgatggtc tgcttcatgg tctgggttcc ctcgaaggtc
      301 ggggcctggg gcggacattc cgcacactgg cacggtgccc aggctggcac gggattatgg
      361 cgcaatgcgg acgggtggtg aacgaaacat ggcgccgccg cgtgacagtt gccagtcctt
      421 cgatcactcc tgcgtcaacc gcgccaccgt ggtgccgccg tcctgcggat tggggcacag
      481 acggtcgagc agcgcggcga ggatacccgg cgcggccagc tcgaagcgga acggcggatt
      541 gatcaccgcc aggccgcagc cgttcagccg ggttggatcg accggctccc gcagcagcag
      601 ttcggcaacc acgacgtcag gaatgccgct ttgctggagc atggcgtgga agccgcgcac
      661 cggggcgcgg tgcttcaccg gataccaggc ggcgaacacg ccggagggga agcgggcctg
      721 ggcggctgcc agggcctggg caaggcggct gaattcctgc gggtcctcga agggcgggtc
      781 gatcagcacc aagccgcggc gttccttcgg cggcagcagc gcgcccagtg cctcccaacc
      841 gtcgcgccgg tgcaccgcga cctgcttgtc gcgggcgaag cggcggcgga gttcgaacgc
      901 gtcctcggga tgcagctcgc agcaggcgag gcggtcgtcc gggcgcagca cggcgcggat
      961 cagggcgggg gagccgggat acaggcccag ccggcgcacc agccggacat agggagccag
     1021 ggcaagcggc gggctatcca gcagccggcc gatgccggag cgccactcac cggtgcgctg
     1081 cgcctccgcg gcgtccaggt cgtaggcgcc cgcgccggca tgggtatcca ggacaaagat
     1141 cggcgccggc ttctgctgca aggcttcgat cagccagagc agcagggcgt gcttcatgca
     1201 atcggcgaaa ttgccggcgt ggaaggcgtg gcgatagttc atcggcgtgg tttccggcgg
     1261 cggtcgcgac cgctttgatg tcggcgatcc ccggccgaag gtccaggccg gacccatgcg
     1321 gcaggcatcc accgcacctt cccccgcatt cgcgcaatcg caggcctgga ccgcaaagca
     1381 gccgcgcggc ggttccgccg gccggttgcg cccgcggaac ctccagcgcg ctacagcccg
     1441 gccgccgcgc gcgccacctg ttccgtggtc gccaggtagg tgtcgatgtc gcggtcttcc
     1501 ggcgccacca gcacgtgctg cacgccggcg tcccgatacg ccgccagcgt gtcatgcaat
     1561 gcgccgacgg tgccggccga acagccgacg cgcagcgaga tggtgaacgc cgcctccggc
     1621 cgcgccgcac gcagccgctg caccatcggc ttcgcctgtt ccggcgccac gcggctgccg
     1681 tgccagccat ccagccgcag cgcccgctgg atggccgcat cggacgaccc gccgatccag
     1741 atcgggatcg gcgcgcaggg ctgcggctgc atgcgcatct cccgcacctc ggccgggatc
     1801 cagcgcgtgg cgaacgacac cggatcgtcg ttccacaccg cgcgcatcag cgcgatacct
     1861 tcatccatgc gccggccacg ctcgcggaac ggcacgccca gtgcggcgaa ctcggcttcc
     1921 atccagccga cgccggcgcc caggatcagc cggccctgcg acagattctg cagggtggcc
     1981 agctccttcg ccagcggcag cggatgccgc atcggcagca ccagcacgct ggtgcccagc
     2041 cgcacccgct tcgtgtacgc ggcggcccag gtcagcgtca gcaccgggtc ccagaaattc
     2101 ggcgacggcg gatacgccgc gtccttcggc acgatgatgt gctcgctgac ccagacatca
     2161 gcgaagccca gcgcctcggc ctgttcggcg gagcggcgga tcgcgtccgg accggccttg
     2221 cggccaatat gcggcaggtg gatgccgagt cgcatgcgtg tcgcctcccg aatctgcccg
     2281 ccacgctgcg ccgctgccct ggctgcgtcc agcgcctgga aaatccgcca tcgagccggg
     2341 ccgagtgcgc cagatgatca tccacgccac gccgggcaga tgccgggcaa cgacaacaag
     2401 gtggcggacg cgttcggtgc tcgcacgagc gtgccgcacc cggcggaata gccggcgcag
     2461 gcttgcctgg ggcgcgtggc gtgcccccgc atccggaagc acgccagcac acctggcgct
     2521 ggccgaaacc tagcgggctt cgtcccagtg ctcgtgccgg tagttccagc cgtagtgctc
     2581 gcgccgccat tgctcgcgcc gccaggcatg ttcccgctcc cagcgccggt gacgccagta
     2641 ctggcagcgc ggaccgcaat ggtcattgtc ccaatcgacg tgctgcaccg tcgccgtcac
     2701 cgccgcctgc ggtgcgggcg cgaggggcgc cgccgccgcg gccagcggca ccaggccgaa
     2761 cacgccggcc agcgcaatac ccgcagtgaa gagtttgtgc atctcggttc ctccttgcgt
     2821 acccgccgga gttgggcacg tctcggccgc aatgaaccgc gggcgggcaa gaaaccggga
     2881 cgggtaaacc attcgtggtc agacagcgca ggcctggaac ctccgcccgc ccatagtgtc
     2941 cgcggcgcgg ctgtcctagt ctggttgccg ccagcggagg agttcgcccg tgatcgacac
     3001 cacgatcacc ctcgaggaca aatacacccg cgcgaccggg cgcgtgctgc tgtccggcac
     3061 ccaggccctg gtgcggctgc cgttgatgca gcgcgcgcgc gacgccgccg cggggctgga
     3121 cacgcgcggc ttcatctccg gctatcgtgg ctcgccgctg ggcaattacg acgccgcgtt
     3181 gtgggatacc ggccgcgcga tccatgaagc cggcatcgtg ttccgccctg gcgtgaacga
     3241 ggaactcgcc gccaccgcgt gctggggcac ccagcaggtc gggctgatcg aaggggcgaa
     3301 gcatgacggc gtgttcgcca tctggtatgg caagggcccg ggcgtcagcc gcgccggcga
     3361 cgcgctgaaa cacggcaatt tcgccgggac cagcccgcat ggcggcgtgc tggtgctggc
     3421 cggcgacgac catgcggcga agagctccac ctccgcccac cagtccgatc cggaactggc
     3481 cgcggc
//
LOCUS       DASSZH010000322        11201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1371400, whole genome shotgun sequence.
ACCESSION   DASSZH010000322 DASSZH010000000
VERSION     DASSZH010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 11201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11201
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371400"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VFN42_06875"
     CDS             complement(<1..196)
                     /locus_tag="VFN42_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET8996375.1"
                     /translation="MTRALIGRTVRRLRQERDLSQQALAARLGISASYLNLIEHDQRA
                     VTASLLIKLGETLRVDLATLS"
     gene            378..1640
                     /locus_tag="VFN42_06880"
     CDS             378..1640
                     /locus_tag="VFN42_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996376.1"
                     /translation="MPMLPAEPIPALSEDCVPPAIIGVAALTTAAVHGATPAMLAAMI
                     ERPDADPIARLYDAGIAHQLAFRCAEGLELQDQALAASLLFRVRRDDGDAGCRLLALV
                     LPGRLMANTPLDFITNHLDVRLDLLFVQPGGALPAGIPDHDIAFFAAGDADDAMIARL
                     GALFDAWPRPALNDPRLLPRLARDRLPTLLAGVPGICAPPAVAASRARLDALLRDGGD
                     IAALLPGCGYPVLVRPLGSHAGHGLRKLESAKDLAAYMTFSFAPDYYVTAFADYRSPD
                     GLYRKYRVAFIDREPFLCHVAASQHWMVHYLNAGMTESADKRADEARAMAQFAVSFAH
                     RHGAAFAALHERLPFDYYAIDCGETPDGRLLVFEADTAAIIHLMDPPEMFPYKHVQMR
                     RVFDAFGAMLERRGRRVRASLRDAAAGD"
     gene            complement(1674..4430)
                     /gene="polA"
                     /locus_tag="VFN42_06885"
     CDS             complement(1674..4430)
                     /gene="polA"
                     /locus_tag="VFN42_06885"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET8996377.1"
                     /translation="MHLILIDGSGFIFRAFHALPPMTRPDGTPVNAVFGFTNMLAKLL
                     REHVGTHIAVIFDAGRTTFRNRLYENYKAHRPPPPEELIPQFALIREVTAAFCVPAIE
                     LEDWEADDLIAAYAVAAEQSGGQVTIVSSDKDLMQLIRPGVDMLDPIKQKPIGPAEVM
                     EKFGVTPDKMVEVQALIGDPVDNVPGVPGIGPKRAAQLINEYGDVERVLAAAPAMKPS
                     KQRDTLIECADRARISRELVQLRADAPLPMPIDRLVACEADKPRLAAWLRLQGFRSIA
                     TRLGLEEAPAAARTVESTARPTQVALPLPMAERPAVEPSAEGFGPYQTITTESALREF
                     LAGTQSCGFLALDVETDALDPMRANLIGFSLATAAGRAAYVPLRHEVLDVQLSRETAI
                     AVLDPVLIDPCVLKIFHHAKFDMLVLTRAGFAMPAPYDDTMLISYAQEAGLHPHGLDE
                     LARLHLGHSPISYDEVTGTGRNRLPFAQVPIDRATAYAAEDADVALRLWQALRPRLRF
                     NHGLALYEQIERRLTGVLMDMERVGIKVDADDLRRMSVDFEQRMGVIEQDCHRLAGRA
                     FNVGSPKQLGEVLFDEMKLTGGKRMKTGAWGTDSSVLQTLADQGSELPARILDWRQLQ
                     KLKSTYADTLVEQINPETGRVHTSFAQAIASTGRLSSNDPNLQNIPIRTEEGSRIRHA
                     FIAEPGHVLVSCDYSQIELRLLAHVADLPALRESFARGEDIHARTASEVFGLPMEGMD
                     PMTRRRAKAINFGIIYGISGFGLARQLGITPGEARGFIDRYFERYPGIRTYMERTKEE
                     ARINGFVTTPFGRRCWVPGIADKNMARRAYAERQAINAPLQGGAADIIKRAMIRLPAH
                     LRAAGLRSRLLLQVHDELLFEAPEDEADRLAGLAREVMESAASLAVPLVVETGKGRTW
                     AEAH"
     gene            complement(4602..5507)
                     /locus_tag="VFN42_06890"
     CDS             complement(4602..5507)
                     /locus_tag="VFN42_06890"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET8996378.1"
                     /translation="MWFHEYRTEKARNGQSIELVLYRKRAGMPRPGEAAPPVLFLVHG
                     SSMSALTSFDLVVPGAGEYSKMNVFARAGFDVWTMDHEGYGKSARTEGNSDIASGVED
                     LRAAMAVVERETGQSRCHFMGDSSGALRAAAYAVAEPQRVGRMVLTALTYTGKGSSTL
                     AKRAEQVDYFRTHHRRLRDRAMIESIFTRDKPGTTDPAVPAAVADMELVHGDTIPTGT
                     YLDMTAHLPVVDPAKIAAPVAVVRGEYDGIASMEDLLDFFGKLPNGDKQFIVIAGAAH
                     ALGTCRQRHAFWHAAHAFLTMPGAA"
     gene            complement(5588..5785)
                     /locus_tag="VFN42_06895"
     CDS             complement(5588..5785)
                     /locus_tag="VFN42_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HET8996379.1"
                     /translation="MPEAPVAPTPTEILHVDSRTVACDGGSGALGHPRVFLYIERDTV
                     MCPYCSRLYVLNPGAGQGGGH"
     gene            5892..6869
                     /locus_tag="VFN42_06900"
     CDS             5892..6869
                     /locus_tag="VFN42_06900"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET8996380.1"
                     /translation="MAVPLIAALLLSGCATTIPTSMLAHYRPVQNGVFIMPDGTTLPY
                     RAWMPPDGHKPKAVVLALHGMNDSRDAWEYPAPELARAGIAVFSPDQAGFGDTATRGE
                     WAGTATLVGDAGVMARRLRHRYPHTRLIVMGESMGAAVAMVAAAQPDPPPVDGYVIIS
                     PAVWGRDEMNLFLRAGLWLFSHAVPGMEVTGAGFVHVKASDNRQAIRRLSDDPLTLHA
                     TRWSAVAGLVNLMDAAQDAAPHMHAPALFMYGGHDELIPPRATAWIWRVLPKQEREAF
                     YPDGYHLLLRDLDRITPTEDIIAWIERPNDPLPSGAHRAAAAWLAKQPK"
     gene            6919..7863
                     /locus_tag="VFN42_06905"
     CDS             6919..7863
                     /locus_tag="VFN42_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996381.1"
                     /translation="MTHLPPEQRMGVAVRSLAAGFYRDGLALHEARREIPDYPEHRLP
                     MRAPAWDARQPLAGRRVLLWHEQGLGDMIQMLRFMPLLAQRGAAIVLAVQPPLKRLAA
                     GLPGVTAVVSPGDSFAEVDFHCPLMSLPHLLGIRLATLPAGVPYLRVPSGLPEAWRSR
                     LGPKLKPRVGLVFSGAQSNTLDRWRSIPARAFAPLLAREDIDFHVLQNEIRAADRDYL
                     AGLPHVRMHEETLTDLSETAGLAMQMDRIITVCTAVAHLSGALGLPTWVLLSTRAHWV
                     WLAGRSDSPWYPTARLFRQQTESDWQPVMQHVAMQLRS"
     gene            complement(7905..9410)
                     /locus_tag="VFN42_06910"
     CDS             complement(7905..9410)
                     /locus_tag="VFN42_06910"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HET8996382.1"
                     /translation="MPFAPPPGLPRCGVVDLGSNSVRLVVYEGECRNPVAIFNEKAVL
                     RLGRGLQSTGRLNPEAMEQAFTVMHRYHAVARAMGAAPFEVLATAAVRDASNGAEFVE
                     RLQARLPGVPIRLISGLEEAEFSAEGVLCGIPSADGILADIGGGSLELVRLDGGRRTA
                     SATLPLGVIRLAERSGNDPVRARAIAEADLASVPWLGEGVGRDLYLVGGAWRALARIH
                     MLQTGYPLHMVHHYSISREEARDLTALIASAGRRALERLPGVPRRRIDDLPYAAVALR
                     RLLRAAGVRRVVFSASGLREGWLMQRLPDEVRSQDPLLAAGRELASRFGRDPSLPPAL
                     LDWTAPLFPQETAGARRLREACCWMSDIGSHDHPEFRAEQGFLRVLRQPGIGLDHHAR
                     AFLALAIAMRYEAETDAAFLAPARLLLDVAAASRAEVLGVALRLAYTLSAGTPDLLAG
                     TALVLRGGQLVLRLQEDSGVFAGESVVRRLDRLAQAVGLEAATETALERAA"
     gene            complement(9415..>11201)
                     /locus_tag="VFN42_06915"
     CDS             complement(9415..>11201)
                     /locus_tag="VFN42_06915"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HET8996383.1"
                     /translation="DEDRGWLEAWFMERLFPVLTPLAIDPAHPFPFIPNMGLVMALLL
                     LREEDAQGMRGLIPLPSQVERFIRLPAAGPEQQIRFVMLEDLVGMFLDRLFPGFRVTG
                     QGMFRLIRDTDVEFEEEAEDLVRSYETALKRRRRGVVIHLGMDARMPEELRTMVADQL
                     DATEDEIHVADGILGVADLRQLIVDDRPDLLFPPYTPRFPERIRDFGGDCFAAIRAKD
                     IIVHHPYESFDVVVQFLRQAAQDPAVIAVKQTLYRTSRDSPIVKALIEAAEAGKFVTA
                     VVELRARFDEEMNIRLARTLEAAGVQVVYGFAELKTHAKLSLVVRREGSSVRSYAHFG
                     TGNYHPITARIYQDLSFFTSDPALTRDAARLFNYMTGYARPAQMEEIAFSPLTTRAVL
                     LELIQKEIAFAHAGRPAGIWIKLNSLVDRQLIDALYAASNAGVKIMAVVRGICCLRPG
                     VPGLSDNIRVKSIIGRFLEHSRICVFGNGHALPSRHARVYISSADWMERNMDWRVESL
                     VPIHNPTVHAQVLDQIMVVNLKDTMQSWELKPDGSWRRVQPGRKPVSAHDYFMTNPSL
                     SGRGSALHGPAVPSPLAMHRRQDRVNQD"
BASE COUNT         1797 a         3856 c         3749 g         1799 t
ORIGIN      
        1 cggacagggt cgccagatcg acccgcaggg tctcgccgag cttgatgagc agagaggcgg
       61 tgacggcacg ctggtcgtgc tcgatcagat tcagataact ggcggagatg cccaggcgcg
      121 ccgccagggc ctgctgggac aggtcccgtt cctggcgcag ccggcgcacc gtccggccga
      181 tcagcgcgcg cgtcatttta catcctttac agaattttcc ccggcttgac agaagcagtg
      241 gccgcagcat tgggagatta gagcacgcgt tcgcaggcgc aatgtcaatt gttgaccagc
      301 gcgctccacg cgcctcccgg ctaaccggcc gttaagcagg ccagcctagg gtgcggcgtt
      361 ccgaaggatg ccgcaccatg ccgatgctcc ccgccgagcc gatcccagct ctcagcgagg
      421 actgcgttcc acccgcaatc atcggcgtgg ccgcgctgac cacagcagcc gtgcatggcg
      481 ccacgcctgc catgctggca gccatgatcg agcggcccga tgccgatccg atcgcccgcc
      541 tgtatgatgc cggcatcgcg caccaactgg cattccgctg cgcggagggg ttggagctgc
      601 aggaccaggc gctggccgcc tccctgctgt tccgcgttcg gcgggacgat ggggatgccg
      661 gatgccggct cctggcactg gtcctgcccg gcaggctgat ggccaatacg ccgctggact
      721 tcatcaccaa ccacctggat gtccgactgg acctgctgtt cgtccagccc ggcggcgcat
      781 tgccggcagg cattccagac cacgacatcg cattcttcgc ggcgggcgat gccgatgacg
      841 cgatgatcgc tcggctgggt gcactgttcg atgcatggcc ccggccggcg ttgaacgatc
      901 cgcgcctgct tccgcggctg gcgcgcgacc ggttgccgac gctgctggcg ggcgtgccag
      961 gcatttgcgc acctcccgct gttgccgcca gccgcgcgcg actggatgcg ctgctgcggg
     1021 acggcgggga catcgcggcg ctgcttcccg gatgcggcta tcccgttctg gtgcgcccac
     1081 tgggatcgca tgccgggcac ggcctgagga agctggagag tgcgaaggat ttggccgcct
     1141 atatgacgtt ttccttcgcg ccggactact acgtgaccgc gttcgccgat taccgttcgc
     1201 ctgacggatt gtatcgcaag taccgcgttg cattcatcga tcgcgaaccc ttcctgtgcc
     1261 acgtggcggc gtcgcagcac tggatggtgc actacctgaa tgccggcatg accgaaagcg
     1321 ccgacaagcg tgccgacgaa gcgcgggcca tggcgcagtt cgccgtctcc ttcgcgcatc
     1381 gccacggcgc tgccttcgcg gcgctgcacg agcggctgcc gttcgactat tatgcgatcg
     1441 actgtggtga aacaccggac ggccgcttgc tggtgttcga ggccgacacg gcggcgatca
     1501 tccacctgat ggatccgccc gagatgtttc cgtacaagca cgtgcagatg cggcgcgtct
     1561 tcgacgcgtt cggcgcgatg ctggagcgac gagggcggcg ggtgcgagca agcctcaggg
     1621 atgcagcggc aggtgactga tcgccgggtg gatcgaacaa cctgctggtg cgatcagtgc
     1681 gcctctgccc aggttcgccc tttgccggtc tccaccacaa gcggcacggc gaggctggcc
     1741 gcactctcca tcacctcgcg cgccaggccg gccagccggt ccgcctcgtc ctccggcgcc
     1801 tcgaacagca gttcgtcatg cacctgcagc agcagccgcg accgtaaccc cgctgcgcgc
     1861 aagtgcgctg ggagacggat catcgcgcgc ttgatgatgt cggccgcacc gccctgcagc
     1921 ggcgcattga tcgcctggcg ctccgcgtag gctcgccgcg ccatgttctt gtcggcaata
     1981 ccgggcaccc agcaccgccg cccgaatggc gtggtgacga agccgttgat gcgggcttct
     2041 tccttggtgc gctccatgta ggtgcggatg ccgggataac gctcgaaata gcggtcgatg
     2101 aaaccgcgcg cctcgcccgg cgtgatcccc aactgacgcg ccaggccgaa tccgctgatg
     2161 ccatagatga tgccgaaatt gatcgccttg gcgcgccgcc gcgtcatcgg gtccatgcct
     2221 tccatcggca gcccgaacac ttcggacgca gtgcgcgcgt ggatatcctc gccccgcgcg
     2281 aagctctcgc gcaacgcggg caggtcggcg acgtgcgcca gcaggcgcag ctcgatctgc
     2341 gaatagtcgc agctgaccag cacgtgccct ggttcggcga tgaaggcatg acgaatgcgg
     2401 ctgccttcct cggtgcggat cgggatgttc tgcagattgg gatcgttcga cgacaggcgg
     2461 ccggtggagg cgatcgcctg cgcgaagctg gtgtgcacgc gcccggtttc cgggttgatc
     2521 tgctccacca gcgtgtcggc gtaggtggat ttcagcttct gcaactgccg ccagtccagg
     2581 atgcgggcgg gcagctccga gccctggtcg gccagcgtct gcagcacgga cgaatcggtg
     2641 ccccaggcac cggttttcat ccgcttgccg cccgtcagct tcatctcgtc gaacagcact
     2701 tccccgagct gcttcgggct acccacgttg aacgcacgcc cggcgaggcg atggcagtcc
     2761 tgctcgatca cgcccatgcg ctgttcgaaa tccaccgaca tgcggcgcag atcgtcggcg
     2821 tccaccttga tgccgacgcg ctccatatcc atcagcacgc cggtcaaacg gcgttcgatc
     2881 tgctcgtaca gcgccagtcc atggttgaag cgcagccgcg gccgcagcgc ctgccaaagc
     2941 cgcaatgcca catccgcatc ctccgccgca taggcggtag cgcgatcgat cggtacctgg
     3001 gcgaagggca accggttgcg gccagtgccg gtgacctcat cgtagctgat cggcgaatgg
     3061 cccagatgca gccgggcgag ttcatccagg ccgtgcggat gcagaccggc ttcctgcgcg
     3121 taggagatca gcatggtgtc gtcgtacggt gccggcatgg cgaagccggc gcgggtcagc
     3181 accagcatgt cgaacttggc gtggtggaag atcttcagca cgcagggatc gatcagtacc
     3241 ggatccagca ccgcgatggc tgtctcgcgc gatagctgca catccagcac ctcatgccgc
     3301 agcggcacat aggccgcgcg gccggccgcg gtggccaacg agaagccgat caggttggcg
     3361 cgcatcgggt ccagcgcgtc ggtctccacg tccagcgcga gaaaaccgca actctgcgtg
     3421 cccgccagga attcccggag tgcgctttcc gtggtgatgg tctggtacgg gccgaacccc
     3481 tccgccgaag gttccaccgc aggccgttcg gccataggca gcggcagcgc aacctgggtc
     3541 gggcgagcgg tgctctccac cgtacgcgcc gcggccggcg cctcctcaag gcccagccgc
     3601 gtggcgatgc tgcggaaacc ttgcagccgc agccaggcgg ccagccgcgg cttgtcggcc
     3661 tcgcacgcca ccagcctgtc gattggcatc ggcaacggcg catcggcgcg cagttgcacc
     3721 agctcgcggg agatgcgggc gcggtcggcg cattcgatca gcgtgtcgcg ctgcttggac
     3781 ggcttcatgg ccggcgcggc ggccagcacg cgctccacgt cgccgtattc gttgatcagc
     3841 tgtgccgcgc gcttcgggcc gatgcccggc acgccgggca cgttgtccac cggatcgcca
     3901 atcagcgcct gcacttccac catcttgtcc ggggtgacgc cgaacttttc catcacctcg
     3961 gccggaccga tcggcttctg cttgatcggg tccagcatat ccacgccggg ccggatcagc
     4021 tgcatcaggt ccttgtcgga ggacacgatg gtcacctgcc cgcccgattg ttccgcggcg
     4081 acggcatagg ccgcgatcag gtcgtcggct tcccagtcct ccagctcgat ggcggggacg
     4141 cagaaggccg cggttacctc gcggatcagg gcgaattgcg ggatcagttc ttccggcggc
     4201 ggcggacggt gggctttgta gttctcgtac agccggttgc ggaaggtggt gcggccggca
     4261 tcgaagatca ccgcgatatg cgtgccgaca tgctcgcgca gcagcttggc cagcatgttg
     4321 gtgaagccga acacggcgtt caccggcgtg ccatccggtc gtgtcatcgg cggcagcgca
     4381 tggaaggcgc ggaaaatgaa gcccgatccg tcgatcagga tcaggtgcaa gggccgctcc
     4441 gccgctgtcg tcgcggcttc gacgtccggc agaagatcag tgtccgcggg cagggccggc
     4501 tccttccgtg cgatgtggga gaccgggcga tgatagcccc gcaggccgct tgatcaaacg
     4561 ccggatcgcc aggcgtccgg cagccgcccc ggggtgccag gtcaggcggc gccgggcatc
     4621 gtcaggaacg cgtgggccgc atgccagaac gcgtgccgct ggcggcaggt gcccagcgcg
     4681 tgcgccgcgc cggcgatcac gatgaactgc ttgtcgccgt tcggcagttt gccgaagaaa
     4741 tccagcaagt cctccatcga ggcgatcccg tcatattcgc cacgcaccac cgccacgggc
     4801 gcagcgatct tcgccggatc gaccaccggc agatgcgccg tcatgtccag ataggtgccg
     4861 gtcgggatcg tatccccatg caccagttcc atgtccgcca ccgcggccgg cacggccgga
     4921 tcggtggtac ctggcttgtc gcgggtgaag atgctttcga tcatcgcccg gtcgcgcagg
     4981 cggcgatggt gggtccggaa ataatcgacc tgctccgcac gcttggccag cgtggatgag
     5041 cccttgccgg tataggtcag cgccgtcagc accatgcgcc ccacacgctg cggctcggcc
     5101 accgcgtagg cggcggcgcg cagcgcgccg gatgaatcgc ccatgaagtg gcagcgcgac
     5161 tggccggtct cgcgctccac caccgccatg gcggcgcgca gatcctccac cccgctggcg
     5221 atatcggagt tgccctcggt gcgcgcggat ttgccgtagc cctcgtggtc catcgtccac
     5281 acgtcgaagc cggcacgggc aaagacgttc atcttcgaat attcgcccgc acccggcacg
     5341 accaggtcga agctggtcag cgccgacatg gacgagccgt gcaccagaaa cagcactggg
     5401 ggcgctgcct cccctggccg tggcatgccg gcacgtttgc ggtagagcac cagctcgatg
     5461 gattgcccgt tacgggcctt ctccgtgcgg tattcgtgga accacaagac gggtgccgag
     5521 tgtgccggct gggcggcggc ggaacggccg ccgtcgaaaa ccccgcccgg ctggggctga
     5581 ttgccgctca gtggccgccg ccctgcccgg ccccggggtt caacacgtac agccgggaac
     5641 aataggggca catcaccgtg tcccgctcga tatagagaaa cacgcgcggg tggcccaacg
     5701 cgccgctgcc gccgtcgcac gcgacggtgc ggctgtccac gtgcaggatc tcggtggggg
     5761 tcggggcgac gggagcctcg ggcatatgcg gttctctgtg gcacaggaag gcggcggatg
     5821 ataccgatat acccgctgct ttcaaccttt gctgagtgcg actgccgaca gtgtcacgag
     5881 taccgcttcg cttggccgtc ccgctgattg ccgcactgct gctgagcggc tgtgcaacaa
     5941 ccatccccac ctccatgctt gcacattacc ggccggtcca gaacggcgtg ttcatcatgc
     6001 cggatggcac gacgctgccc taccgggcct ggatgccacc ggacggacac aagcccaagg
     6061 cggtggtgct ggcgctgcac ggcatgaacg acagccgtga cgcctgggaa taccccgcac
     6121 ccgaactggc gcgtgccggg atcgcggtat tctcccccga ccaggcgggc ttcggcgaca
     6181 ccgccacccg cggcgaatgg gcggggaccg cgaccctggt gggcgatgcc ggggtgatgg
     6241 cgcgccggct gcgccaccgt tacccgcaca cgcggctgat cgtgatgggg gagagcatgg
     6301 gtgccgccgt ggcgatggtg gccgcagccc agcccgaccc gccgcccgtt gatgggtatg
     6361 tcatcatctc cccggcggtg tggggcaggg acgagatgaa cctgttcctg cgcgccgggc
     6421 tatggctgtt ttcccacgcc gtgccgggga tggaagtcac tggcgccggc ttcgtccacg
     6481 tgaaggcgag tgacaaccgc caggcgatcc ggcgcctgtc cgacgatccg ctgaccttgc
     6541 acgccacgcg ctggtccgcg gtggccgggc tggtgaacct gatggacgcc gcgcaggatg
     6601 ccgccccgca catgcacgcg ccggcactgt tcatgtatgg cggacatgac gagctgattc
     6661 cgccccgcgc caccgcctgg atctggcggg tcctgccgaa gcaggagcgg gaggcgtttt
     6721 atccggatgg ctaccacctg ctgttgcgcg acctggatcg catcacgccg accgaggaca
     6781 tcatcgcctg gatcgaacgg ccgaacgatc ccctgccctc cggcgcccac cgggcagcgg
     6841 cagcttggct ggcgaagcag ccgaagtgag caaccggcct gaggacgcag gttgagatcg
     6901 tctctgctgg cgcggggcat gacccacctg ccgcccgaac agcggatggg ggtcgccgtg
     6961 cgctccctcg ccgcaggttt ttatcgcgac ggcttggcgc tgcacgaagc gcgccgggag
     7021 attccggatt accccgaaca ccgcctgccg atgcgcgcgc cggcatggga tgcccgccaa
     7081 ccgctcgccg gccgccgggt gctgctgtgg cacgagcagg ggctggggga catgatccag
     7141 atgctgcgct tcatgccgct gctggcgcaa cgcggcgccg cgatcgtgct cgccgtgcag
     7201 ccgccgctga aacggcttgc tgccggtctg cccggggtaa ctgccgtggt gtcgccgggc
     7261 gacagcttcg cggaggtgga tttccactgt ccgctgatga gcctgcctca tctgctcggc
     7321 atccgcctgg cgacgcttcc ggcgggtgtg ccgtatctgc gcgtgccatc cgggttgccg
     7381 gaggcatggc gatcgcggct tgggccgaag ctgaaaccga gggtcgggtt ggtgttctcc
     7441 ggggcgcaga gcaacacgct ggaccgctgg cggtcgatcc cggcgcgcgc cttcgcgccg
     7501 ctgctcgcac gggaggacat cgatttccac gtgctgcaga acgagatccg cgcggccgac
     7561 cgggactacc tcgccggact gccgcatgtg cggatgcacg aggagacgct gaccgacctg
     7621 agcgagaccg ctggattggc catgcagatg gaccggatca tcaccgtgtg caccgccgtg
     7681 gcccacctgt ccggcgcgct gggtctgccg acatgggtgc tgttgtccac ccgggcgcac
     7741 tgggtctggc tggccgggcg aagcgattcg ccgtggtacc cgacggcgcg gctgttccgg
     7801 cagcagaccg agagcgactg gcaaccggtg atgcagcacg tcgcaatgca gctacgaagc
     7861 tgacccaggc aaattccccc tgccgaaacg gggaggtttt tctgtcaggc tgcccgctcc
     7921 agcgccgttt cggtcgccgc ctccagcccc accgcctgag ccagccggtc cagccggcgc
     7981 accacgctct ccccggcgaa cactccgctg tcctcctgca ggcgcaacac caattgtccg
     8041 ccgcgcagca ccagggcagt gccggccagc aggtcgggcg tgccggccga cagcgtgtag
     8101 gccagccgca gcgccacacc cagcacttcc gcccggctgg cggccgccac atccagcaac
     8161 aggcgggcgg gcgcgaggaa cgccgcatcg gtctccgcct cgtaccgcat ggcgatggcc
     8221 agcgcgagga aggcccgtgc atggtggtcc agcccgatgc ccggttggcg caggacccgc
     8281 aggaaccctt gttcggcacg gaattccggg tggtcgtggc tgccaatgtc cgacatccag
     8341 cagcacgcct cccgcagccg gcgcgccccg gcggtttcct gcggaaacag cggcgcggtc
     8401 cagtcgagca gcgcgggcgg cagtgacgga tcgcggccga accgcgaggc cagctcgcgg
     8461 ccggcggcaa gcagcgggtc ctggctccgc acctcgtccg gaagccgctg catcagccag
     8521 ccttcccgca gcccgctggc gctgaacacc acccgccgca cgccggccgc ccgcagcagc
     8581 cggcgcagcg ccaccgcggc atagggcagg tcgtcgatgc ggcgccgcgg cacacccggc
     8641 agccgctcca gcgcccgccg cccggcggag gcgatcagtg ccgtcaggtc gcgcgcctcc
     8701 tcccggctga tgctgtaatg gtgcaccatg tgcagcggat agccggtctg cagcatgtgg
     8761 atgcgggcca gcgcacgcca ggcaccaccc accagataca ggtcacgccc cacgccctcc
     8821 ccaagccagg ggaccgaggc cagatcggct tcggcgatgg cgcgggcccg caccgggtcg
     8881 ttgcccgacc gctcggccag ccggatcacg cccagcggca gcgtggcgga tgcggtgcgc
     8941 cggccgccat ccagccgcac gagctcgagc gagccgccgc cgatatcggc aaggatcccg
     9001 tccgcgctgg gaatgccgca cagcacgccc tcggcggaga attcggcctc ctccagcccg
     9061 ctgatcagcc ggatcggcac gccgggcagc cgagcctgca agcgctcgac aaattccgca
     9121 ccgttggacg catcacgcac ggcggcggta gccagcacct cgaagggcgc ggcacccatt
     9181 gcccgcgcga ccgcgtggta gcgatgcatg acggtgaaag cctgctccat cgcctccggg
     9241 ttcagccggc cggtactctg caagccgcga cccagccgga gcaccgcctt ctcgttgaag
     9301 atcgccaccg gattgcggca ctcgccttcg tacacaacca gacgcaccga gttcgaacca
     9361 aggtccacca cgccgcaacg cggcaatccg gggggcggtg cgaagggcat gcggtcagtc
     9421 ctggttcacc cggtcctgcc gccggtgcat cgccagcggc gagggcaccg ccggaccatg
     9481 cagtgccgat ccgcggccgg acaaagaggg gttggtcatg aagtagtcat gcgccgaaac
     9541 cggcttccgt ccaggctgca cccggcgcca ggaaccgtcg ggcttcagtt cccaggactg
     9601 catcgtgtcc ttgaggttca ccaccatgat ctgatcgagc acctgcgcat gtacggttgg
     9661 attgtggatc ggcaccaggg actcaacgcg ccaatccatg ttgcgctcca tccagtcggc
     9721 ggagctgata tacacccgcg cgtgccggct gggcagggca tggccgttgc cgaacacgca
     9781 gatgcggctg tgctccagga accgcccgat aatggacttc acccggatgt tgtcggacag
     9841 gcccggcaca ccgggccgca ggcagcagat gccgcgcacc accgccatga tcttcacccc
     9901 ggcattggag gccgcataca gggcgtcgat caactgccgg tccaccagcg agttcagctt
     9961 gatccagatg ccagccgggc gaccggcatg cgcgaaggcg atctccttct ggatcagctc
    10021 cagcagaact gcgcgggtgg tcagcgggct gaacgcgatc tcctccatct gcgccggacg
    10081 ggcatagccg gtcatgtagt tgaacagccg ggcggcatcg cgcgtcagag cgggatcgga
    10141 ggtgaagaag ctcaggtcct ggtagatgcg cgcggtgatc gggtgatagt tgccggtgcc
    10201 gaaatgcgcg taggagcgca ccgagctacc ttcccggcgc accaccagcg acagcttcgc
    10261 atgcgtcttc agctcggcga agccatagac cacctgcacg ccggcggctt ccagtgtgcg
    10321 ggcgaggcgg atgttcattt cctcgtcgaa ccgggcgcgc aattccacca ccgcggtgac
    10381 gaacttgccg gcttcggcgg cctcgatcag cgccttgacg atggggctgt cacggctggt
    10441 gcggtacagc gtctgcttca ccgcgatcac cgccgggtcc tgcgccgcct ggcggaggaa
    10501 ctgcaccacc acgtcaaaac tctcatacgg gtgatggacg atgatgtcct tggcgcggat
    10561 cgccgcgaaa caatcgccgc cgaagtcgcg gatgcgttcc gggaagcgcg gcgtgtaggg
    10621 cgggaacagc aggtccggcc ggtcgtcgac aatcagttgc cgcaggtcgg cgacacccag
    10681 tatgccgtcg gcgacatgga tctcgtcctc ggtggcgtcg agctggtccg ccaccatcgt
    10741 ccgcagctcc tccggcatgc gggcgtccat gcccagatgg atcaccacac cgcgccggcg
    10801 ccgcttcagc gccgtttcgt aggagcggac gaggtcttcc gcttcttcct cgaactccac
    10861 gtcggtgtcg cggatcagcc ggaacatgcc ctggccggtg acccggaatc cggggaacag
    10921 ccggtcgagg aacatcccga ccaggtcctc cagcatgacg aagcggatct gctgctcggg
    10981 tcccgcggcg gggagccgga tgaagcgctc cacctggctg ggcagcggga tcaggccgcg
    11041 catgccctgc gcgtcttcct cacgcagcag cagcagggcc atcaccaggc ccatgttggg
    11101 gatgaacgga aatggatggg ccggatcgat cgcaagcggc gtcagcaccg gaaacagccg
    11161 ctccatgaac caggcctcaa gccaaccgcg atcctcgtcg c
//
LOCUS       DASSZH010000323         3046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_195237, whole genome shotgun sequence.
ACCESSION   DASSZH010000323 DASSZH010000000
VERSION     DASSZH010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195237"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VFN42_06920"
     CDS             complement(<1..636)
                     /locus_tag="VFN42_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET8996384.1"
                     /translation="MSETKTQAADLVPVTVLTGYLGAGKTTLLNRILSEQHGRKYAVV
                     INEFGELGVDNDLVVDTDEEVFEMNNGCICCTVRGDLIRIVGGLMKRRDKFDGIIIET
                     TGLANPAPVAQTFFVDEGVRARTRLDAVVTVVDAKNLPARLEDSHEAEDQIAFADVIV
                     LNKTDLVTPEELEEVERKIRTINKFATIHRTQRSAVPIEQVLDRGAFDLNRV"
     gene            complement(743..2017)
                     /gene="serS"
                     /locus_tag="VFN42_06925"
     CDS             complement(743..2017)
                     /gene="serS"
                     /locus_tag="VFN42_06925"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET8996385.1"
                     /translation="MHDIRTIRTDPAGFDAAMARRGMAAVSPEILALDGERRAAQTAL
                     QDKQARRNALSKEVGQGKRAGADTSGLEAEATALRGEMEALEKRATELDDRIRGILES
                     LPAVLDSDVPDGPDESDNVVLKQVGEPRDLGFLPKQHFELGEAIGQMDFATAAKLAGA
                     RFVVLRGALARMERALGQFMLDLHTREHGYTEMAVPSLVNDAAVYGTAQLPKFSEDLF
                     RTTDGRWLIPTAEVPLTNTVAGEIIAEKDLPIRITALTDCYRAEAGAAGRDTRGMLRQ
                     HQFRKVEMVSIVHPDSSAAEQERMVSCAEKVLALLKIPFRRVVLSSGDTGFASARTFD
                     LEVWLPGQQAWREISSCSNIRDFQARRMNARFRPAEGKGTAYVHTLNGSGVAVGRALI
                     AVMENHQQADGSILIPEVLWPYMGGMERIAPG"
     gene            complement(2073..2873)
                     /gene="tatC"
                     /locus_tag="VFN42_06930"
     CDS             complement(2073..2873)
                     /gene="tatC"
                     /locus_tag="VFN42_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HET8996386.1"
                     /translation="MSDTSRMADIEEDPIHDKPMPLLEHLLELRRRLLWSVVAFFLCF
                     FGAYYFSGQIYYFLAEPLANVLRSQGMPDPHLIYTQLYEAFFTKIKVAFFGAAFLSFP
                     IIAAQIYLFVAPGLYRSEKKALLPFLIATPVLFVMGAALAYYFVFPFAWKFFASFQTP
                     TGGGGMPIELLPRVSDYLDLVMKLIFAFGITFQVPVALTLLAKVGIVTSKGLKRFRRY
                     AYVGMFVIAAILAPPDVITQCGLALPLLALYEISIISAKFVEPKPVEV"
BASE COUNT          505 a         1020 c         1013 g          508 t
ORIGIN      
        1 gacgcggttg aggtcgaagg cgccgcggtc cagcacctgc tcgatcggca ccgcagagcg
       61 ctgggtgcgg tggatggtgg cgaacttgtt gatggtgcgg atcttgcgct ccacctcttc
      121 gagttcttcc ggcgtcacca ggtcggtctt gttcagcacg atgacgtcgg cgaaagcgat
      181 ctggtcttcc gcctcgtgac tgtcctccag ccgcgccggc aggttcttcg cgtccaccac
      241 cgtgaccacc gcgtccagcc gcgtcctggc gcgcacgcct tcatcgacga agaaggtctg
      301 cgccaccggc gccgggttgg ccaggccggt ggtctcgatg atgatgccgt cgaacttgtc
      361 gcgccgcttc atcaggccgc cgacgatgcg gatcaggtcg ccgcgcacgg tgcagcagat
      421 gcagccgttg ttcatctcga acacttcctc gtcggtgtcc accacgaggt cgttgtccac
      481 gccgagttcg ccgaactcgt tgatcaccac ggcgtatttg cggccgtgct gctcgctgag
      541 gatgcgattc agcagggtcg tcttgccggc gcccagatag ccggtgagca ccgtcaccgg
      601 tacgaggtct gcggcctggg tcttggtctc cgacattgtt ggctgtgctc cgggaagatc
      661 gggagagggt tacgccagat atagggtgtg tggcaccgtg ggaccaccgg gtgggccggg
      721 cggccggaag gatccccctc gttcagcccg gggcgatccg ctccatgccg cccatatacg
      781 gccacagcac ttccgggatc aggatcgacc cgtccgcctg ctgatggttc tccatcaccg
      841 cgatcaaggc ccgccccacc gccacgccgg aaccgttcag cgtgtgcaca taggcggtgc
      901 ccttgccctc cgcggggcgg aaacgggcat tcatccgccg cgcctggaag tcgcggatgt
      961 tcgagcagga gctgatctcc cgccacgcct gctgccccgg cagccacact tccagatcga
     1021 aggtgcgggc cgaggcaaat ccggtatcgc cggacgacag caccacccgg cgaaacggga
     1081 tcttcagcag ggccagcacc ttctcggcgc agctcaccat ccgctcctgc tcggcggcgc
     1141 tgctgtcggg gtgcacgatg gaaaccatct ccaccttgcg gaactggtgc tggcgcagca
     1201 tgccgcgggt gtcgcggccc gcagcacccg cctcggcgcg gtagcaatcg gtcagcgcgg
     1261 tgatgcggat cggcaggtcc ttctccgcga tgatctcgcc ggccaccgtg ttggtcagcg
     1321 gcacctctgc ggtagggatc agccagcggc cgtcggtggt gcggaacagg tcctcggaga
     1381 atttcggcag ctgcgccgtg ccgtaaaccg cggcgtcgtt caccagcgac ggcaccgcca
     1441 tttcggtata gccgtgctcg cgcgtgtgca ggtccagcat gaactggccc agcgcgcgct
     1501 ccatgcgggc cagcgcgccg cgcagcacga cgaaacgggc gccggccagc ttggcggcag
     1561 tggcgaagtc catctggccg atcgcttcgc ccagttcgaa atgctgcttc ggcaggaagc
     1621 cgaggtcgcg cggctcgccc acctgcttca gcaccacgtt gtcggattca tccggcccgt
     1681 ccggcacgtc ggaatccagc accgccggca ggctctccag gatgccgcgg attctgtcgt
     1741 ccagctcggt cgcgcgcttt tccagcgcct ccatctcacc gcgcagcgcg gtggcctccg
     1801 cctccagccc ggaggtatcg gcgccggccc gcttgccctg gccgacttcc ttcgacagcg
     1861 cgttgcgccg cgcctgcttg tcctgcagcg cggtctgcgc cgcgcggcgc tcgccatcca
     1921 gcgccaggat ctctggcgac accgccgcca tgccgcgccg cgccatggcg gcatcgaagc
     1981 cggcgggatc ggtccggatg gtgcggatgt cgtgcatgga gggaacctgg ctggagacga
     2041 ggggaggagg agtccttacg cggcgccggc cgtcacacct ccacgggctt cggctcgacg
     2101 aacttcgcgg aaatgatgga aatctcgtac agtgccagca acggcagcgc gagcccacat
     2161 tgggtgatga cgtcgggcgg cgccaggatt gcggcgatca cgaacatgcc gacataggcg
     2221 tagcgccgga accgcttcag gcccttggaa gtgacgatcc ccaccttggc cagcagcgtc
     2281 agcgccaccg gcacctggaa ggtgatgccg aaggcgaaga tcagcttcat caccaggtcg
     2341 aggtagtcgc tcacccgcgg cagcaactcg atcggcatgc caccgccgcc ggtcggcgtc
     2401 tggaaactgg cgaagaactt ccaggcgaag gggaacacga agtagtacgc cagcgccgcc
     2461 cccatcacga acagcaccgg cgtcgcaatc aggaacggca gcagcgcctt cttttcgctg
     2521 cgatacaggc cgggcgcgac gaacaggtag atctgcgcgg cgatgatcgg aaacgacagg
     2581 aacgccgcgc cgaagaacgc caccttgatc ttggtgaaga acgcttcgta cagctgggtg
     2641 tagatcagat gcgggtccgg catgccctgc gaccgcagga cgttggccag cggctccgcc
     2701 aggaaatagt agatctggcc ggagaagtag taggcaccaa agaagcacag gaagaacgcc
     2761 accacggacc acagcagcct gcggcgcaat tcgagcaggt gctcgagcag cggcatcggc
     2821 ttgtcatgaa tcggatcttc ttcgatgtcg gccatgcggc tggtgtcact catgatcggt
     2881 cagtcacgcc tgcgggtggg atcggcgttc cagcgcgacc gccgggggga tgaaagccgg
     2941 cggctccacg tcgctgtccg ccgcgcactg ctgcgccagc agcgattccg gcggcacggc
     3001 gttgggcggg atgaaggccg gcgccacgat ggcggcgtgc tgctcc
//
LOCUS       DASSZH010000324         5408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_706565, whole genome shotgun sequence.
ACCESSION   DASSZH010000324 DASSZH010000000
VERSION     DASSZH010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706565"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VFN42_06935"
     CDS             complement(<1..188)
                     /locus_tag="VFN42_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET8996387.1"
                     /translation="MNIHEYQAKELLKRYGVAVPDGHVAWTAEEAVEAAKRLPGPVYV
                     VKSQIHAGGRGAGRFADDP"
     gene            complement(393..1346)
                     /gene="mdh"
                     /locus_tag="VFN42_06940"
     CDS             complement(393..1346)
                     /gene="mdh"
                     /locus_tag="VFN42_06940"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET8996388.1"
                     /translation="MARNKIALIGAGNIGGTLAHLAGLKELGDVVLFDVFGGVAAGKA
                     LDIMQSGPVEGFDAMMTGGSDYSAIKGADVVIVTAGFPRTPGMSRDDLIGKNAGVVAQ
                     VAEGIRNNCPDAFVICITNPLDAMVWVMQQKSGLPANKVVGMAGVLDSSRFRLFLAQE
                     FGVSVEDVTAFVLGGHGDSMVPLTRYSTVAGIPVPDLIKMGWTTQAKIDAIVQRTANG
                     GGEIVKLLERGSAFYAPAASAIAMAESYLRDKKRVLPCAAYLNGEYGIKDLYVGVPVV
                     IGAGGIERIVEIELNAEEKAAFDKSCAAVRELIDASRKLVG"
     gene            complement(1801..2694)
                     /locus_tag="VFN42_06945"
     CDS             complement(1801..2694)
                     /locus_tag="VFN42_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET8996389.1"
                     /translation="MNGGIRPGFLSRLRALLPRRYPEQSLRASIAELVQEAAQEQVLP
                     GEAQELDRHERLLIANVLRLREMTAADVMVPRADIIAIRADVTLAQAIELFRTDGHSR
                     MPVFRESLDDVVGMVHLKDLFAYVGRPEAFSLEAIMRRPLMVAPQMPVLELLLQMRQQ
                     RIHLALVVDEYGGIDGLVTIEDLVETITGDIADEHDDPAVPMLVERPDGSLDVNARLP
                     VEEFEQKVGPVLSEDERDADIHTVGGLVFTLAGRVPAKGEVLSHHSGIEFRVLDADPR
                     HIRRVRVRRPAAPETEKAAAE"
     gene            complement(2691..3146)
                     /gene="ybeY"
                     /locus_tag="VFN42_06950"
     CDS             complement(2691..3146)
                     /gene="ybeY"
                     /locus_tag="VFN42_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET8996390.1"
                     /translation="MDPTGSRCPAEAGDPDVEVIVEEPAWRRFVPGAAFLAARAAHAA
                     GATGTVVLANDRMVRRLNARHRGRNKPTNVLTYTAPAPEVILALGVVRREARRFGRRP
                     AHHLVHLVVHGALHLAGQDHIRPGEARRMEMAEARILHRLRVPNPWKQA"
     gene            complement(3347..4402)
                     /locus_tag="VFN42_06955"
     CDS             complement(3347..4402)
                     /locus_tag="VFN42_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET8996391.1"
                     /translation="MSHVSASATTSQSPPGGKAVTLQFSDNTLLPLLLGDHDRHLVRI
                     EQALGVRMSCRGNRVAIAGDAARVEAAQSMLQSLWRRLERGESVGRPEVEAAIRLMDS
                     ETDPRLPLSDLPAIHTRRGAVAARSPGQAEYMEALASQDMVFGVGPAGTGKTYLAVAQ
                     GVAMLQAGKVDRIVLSRPAVEAGERLGFLPGDMKEKVDPYLRPLYDALHDMLPGEQVI
                     RRMNNGEIEVAPLAFMRGRTLAHCYAILDEAQNTSPVQMKMFLTRMGEGTRMVVTGDL
                     SQIDLPGGVRSGLRDALDTLEGISGIRVVRFDKRDVVRHPLVTAIVDAYDQRAAAEGD
                     KRREARRSRDGGEPSGK"
     gene            complement(4399..>5408)
                     /locus_tag="VFN42_06960"
     CDS             complement(4399..>5408)
                     /locus_tag="VFN42_06960"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012812834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035596 - methylthiotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HET8996392.1"
                     /translation="PEMVARATRAAGAVIDTDFPAEQKFDFLPDAAAPQGVCAFLSIQ
                     EGCDRFCSFCVVPYTRGAEQSRPAASVLVEAHRLVAQGAREITLLGQNVNAWHGAAPD
                     GGEWDLGRLIRELADISGLLRLRYATSHPRDMTASLIAAHRDVTLLMPFLHLPVQAGS
                     DRILAAMNRRHRAADFLRIVEKLRAARPDLALSSDFIVGHPGETEADFQATLDLVRQV
                     GFAQAFSFRYSARPGTPAAAAPGQVPEDEKERRLQELQALLRDQQARFNAGCVGTALP
                     VLFTGPGRHPGQMAGRSPFLQPVHVSGAESLLGKEISVRIAAALPNSLAGTLIQERAS
                     A"
BASE COUNT          809 a         1900 c         1855 g          844 t
ORIGIN      
        1 gggtcgtcgg cgaagcggcc ggcgccgcgc ccgccggcgt ggatctgcga cttcaccaca
       61 tagaccggtc cgggaagccg cttcgccgct tcaaccgctt cttccgcggt ccaggcaacg
      121 tggccatcgg gcaccgcgac gccgtagcgc ttcagcagtt ccttggcctg gtattcgtgg
      181 atgttcattg ctcagcccca tcagccgcgg cgcggcgtac gacgtcatgg cgggcgcggg
      241 cccgccatcc acgactggct atgggagtgt catagccgca ggccgctttg gcagtgttta
      301 tcaggaagtc ttggatggcg ggcctgcgcc cgccacaccg ctgaccaatg gtcagggtcg
      361 agtcacttcg tgacacgaac gccctcaccg gctcagccga cgagcttcct ggatgcgtcg
      421 atcagttcgc gcaccgcggc gcaggatttg tcgaacgccg ccttctcctc ggcattcagc
      481 tcgatctcga cgatgcgctc gatgccgccg gcgccgatca ccacgggcac gccgacatac
      541 aggtccttga tgccatattc gccgttcaga tacgcggcgc agggcagcac gcgcttcttg
      601 tcgcgcaggt agctctccgc catggcgatc gccgatgccg ccggggcgta gaaggcgctg
      661 ccacgctcca gcagcttgac gatctcgccg ccgccattgg cggtgcgctg cacgatggcg
      721 tcgatctttg cctgcgtggt ccagcccatc ttgatcaggt cgggcaccgg aatgccggcg
      781 acggtggaat agcgggtcag cggcaccatg ctgtcgccgt ggccgcccag cacgaaggcg
      841 gtgacgtctt ccaccgagac gccgaactcc tgcgccagga acaggcggaa gcgggagctg
      901 tcgagcacgc cggccatgcc caccaccttg ttggccggca ggccggattt ctgctgcatc
      961 acccagacca tcgcgtcgag cgggttggtg atgcagatga cgaacgcgtc ggggcagttg
     1021 ttccggatgc cctcggcgac ctgggcgacg acgccggcgt tcttgccgat caggtcgtcg
     1081 cggctcatgc cgggggtgcg cgggaagccg gcggtgacga tcaccacgtc ggcgcccttg
     1141 atggcactgt agtcggagcc gccggtcatc atggcgtcga agccctcgac cgggccggac
     1201 tgcatgatgt cgagcgcctt gccggcggcg acaccgccga acacgtcgaa cagcacgacg
     1261 tcgcccagtt ccttcaggcc ggccagatgg gccagcgtgc cgccgatatt gccggcgccg
     1321 atgagggcga tcttgttgcg tgccatgtgt cagtgtcctt cccgagggcg ccggtcggta
     1381 tcgcggcggt gatgtggcta cggccccggt cgctggtgcg gcggggcgga acgcgtgggg
     1441 tgactagcgg attggccgcg gggcggcaag caaggcgggg cggcgggaca ggggtgatcg
     1501 ggtgaaccac gcggtctgga ctaccctctg cgcggccgat caagtccggg gactgaacgg
     1561 gccggtcatc cattggcgtg ctttataggc atcggctcgc gtccgcatga agcccgcgcc
     1621 tgcgccgctt ggcccctggc actggcggca ccgcaaagca agatttcttg ccgcagatgc
     1681 acgcagatga acgcggatgc gatccggacc agccgacact gctgcactcc ctgctcagtc
     1741 gtagatgccg cctcgcgggc cgagccgtgt gatcgtgatc gccggcgaca actgccttgc
     1801 ctactccgcc gccgcctttt ccgtctcggg tgcggccggc cggcggaccc gcacccggcg
     1861 gatatgccgc gggtccgcat ccagcacgcg gaactcgatg ccggagtggt ggctcagcac
     1921 ctcgcccttc gccggcacgc gcccggccag ggtgaacacc agcccgccca ccgtgtggat
     1981 gtcggcgtcg cgctcgtcct cgctcagcac cgggccgacc ttctgctcga attcttcgac
     2041 cggcaggcgg gcgttgacgt ccagcgagcc gtccgggcgt tccaccagca tcggcaccgc
     2101 gggatcgtcg tgctcgtcgg cgatgtcgcc ggtgatcgtc tccaccaggt cctcgatcgt
     2161 caccagcccg tcgatgccgc cgtactcatc caccaccagc gccaggtgga tgcgctgctg
     2221 gcgcatctgc agcagcagtt ccagcacggg catctgcggc gccaccatca gcggccggcg
     2281 catgatcgcc tccagcgaga aggcttccgg ccggccgaca taggcgaaca ggtccttcag
     2341 atgcaccatg cccaccacgt catccagcga ttcccggaac accggcatgc gggaatggcc
     2401 gtcggtgcgg aacagctcga tcgcctgcgc caaggtcacg tcggcgcgga tcgctatgat
     2461 gtcggcgcgc ggcaccatga cgtcggcggc ggtcatttcc cgcagccgca gcacgttggc
     2521 gatcagcagc cgttcgtgcc ggtcgagttc ctgcgcctcg cccggcagca cctgctcctg
     2581 tgccgcttcc tgcaccagtt cggcgatgga cgcgcggagc gactgctccg gatagcggcg
     2641 cggcagcagc gcgcgcaggc gggacagaaa gccgggcctg atgccgccgt tcatgcctgt
     2701 ttccacggat tgggcacgcg cagccggtgc aggatgcggg cttccgccat ttccatgcgg
     2761 cgcgcctcgc cggggcggat atggtcctgc ccggccaggt gcaaggcgcc gtgcaccacc
     2821 aggtgcacca gatgatgcgc cggccggcgg ccgaaacgcc gcgcctcacg ccggacgacg
     2881 cccagcgcca ggatcacctc gggcgccggg gcggtgtaag tcagcacgtt ggtgggcttg
     2941 ttgcggccgc ggtggcgcgc attcagccgg cgcaccatgc gatcgttggc cagcaccacg
     3001 gtgccggtcg ccccggccgc atgcgcggcg cgggcggcaa ggaacgccgc cccgggaacg
     3061 aaccggcgcc aggccggctc ttcgacgatc acctccacgt ccgggtcccc cgcttccgcg
     3121 gggcatctac ttcctgtcgg gtccatgtcc ttcgagccgc ttgccggccc ccagccccga
     3181 acccacctgg gtactgggtt cggcaatatc cgcgaccggg gagacggcct ccggatcaga
     3241 ataccacatc ggcgggagaa tcgcgtggcc caggcggctc actgcctgga tcccagcccc
     3301 gcacagcgtt gcgcgcatgc gccggcgcag ccgctccatc tcctcctcac ttcccgctcg
     3361 gctcgccgcc atcgcgtgac cggcgggcct cgcgccgctt gtcgccctcc gccgcggcgc
     3421 gctggtcgta ggcgtcaacg atcgcggtca ccagcgggtg ccggaccacg tcgcgcttgt
     3481 cgaagcgcac cacccggatg ccggagatgc cctccagcgt gtccagcgca tcgcgcaggc
     3541 cggaacgcac gccgccgggc aggtcgatct gcgacaaatc gccggtgacc accatgcggg
     3601 tgccctcgcc catccgggtg aggaacatct tcatctgcac cggggaggta ttctgcgcct
     3661 cgtccaggat ggcatagcaa tgcgccaggg tgcggccgcg catgaaggcc agcggggcga
     3721 cctcgatctc gccgttgttc atccggcgga tcacctgctc gcccggcagc atgtcgtgca
     3781 gagcgtcgta cagcgggcgc aggtagggat cgaccttctc tttcatgtcg cctggcagga
     3841 agcccaaccg ctcgccggct tccaccgcgg ggcgagacag cacaatgcgg tccaccttgc
     3901 cggcctgcag catcgccacg ccctgggcga ccgccaggta ggtcttgccg gtgccggccg
     3961 ggccaacccc gaacaccatg tcctgcgagg ccaatgcctc catgtactcg gcctggcccg
     4021 ggctgcgggc ggccactgcg ccgcggcggg tgtggatcgc cggcaggtcg gacagcggca
     4081 gacgcgggtc ggtctcgcta tccatcaacc ggatcgccgc ctccacttcc ggccggccga
     4141 cgctctcgcc gcgctccagc cggcgccaca ggctttgcag catgctctgc gcggcctcga
     4201 cgcgggcggc atcgccggcg atggcgaccc ggttgccgcg gcaggacatg cgcacgccca
     4261 gggcctgctc gatgcggacc agatgccggt catggtcgcc gagcagcagg ggcagcagcg
     4321 tgttgtcgct gaattgcagg gtgacggcct tgccgccggg cggggactga gacgtcgttg
     4381 ctgacgcgga cacgtggctc aagcggaggc tcgctcctgt atcagggttc cagccaaaga
     4441 attggggagt gcagcggcga ttcgcacgga aatttccttc ccaagcagag attccgcacc
     4501 agaaacatgc accggctgca agaacggcga gcggccggcc atctgcccgg gatggcggcc
     4561 cggcccggtg aacagcaccg gcagcgccgt gccgacgcag ccggcgttga accgggcctg
     4621 ctggtcgcgc agcaacgcct gaagttcctg caggcggcgc tccttctcgt cctccggcac
     4681 ctggccgggc gccgcggccg ccggcgtgcc ggggcgcgcc gagtagcgga agctgaaggc
     4741 ctgggcgaag ccaacctggc gaaccaggtc cagcgtggcc tggaagtcgg cctcggtctc
     4801 gcccggatgg ccgacgatga agtcggagga cagcgccagg tcggggcggg cggcgcgcag
     4861 cttctcgaca atgcgcagga agtcggccgc gcgatgccgg cggttcatcg ccgccaggat
     4921 gcggtcggag ccggcctgca ccggcagatg caggaacggc atcagcagcg tcacgtcccg
     4981 gtgcgcggcg atcagcgagg cggtcatgtc acgcgggtgt gacgtggcgt agcgaagccg
     5041 caacaggccg gaaatgtcgg ccagttcgcg aatcagccgc cccagatccc attcgccgcc
     5101 gtccggtgcc gccccatgcc aggcgttgac gttctggccg agcagcgtga tctcgcgggc
     5161 gccttgcgcc accaggcggt gggcttccac cagcaccgaa gctgctggcc ggctttgttc
     5221 cgcgccgcgg gtatagggca ccacgcagaa gctgcagaac cggtcgcagc cctcctggat
     5281 gctgaggaag gcacagacgc cctgcggtgc cgcggcgtcg ggcaggaagt cgaatttctg
     5341 ctccgccggg aaatcggtat cgatcaccgc cccggcggcg cgggtggcgc gggccaccat
     5401 ctcgggca
//
LOCUS       DASSZH010000325         3521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_382924, whole genome shotgun sequence.
ACCESSION   DASSZH010000325 DASSZH010000000
VERSION     DASSZH010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382924"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VFN42_06965"
     CDS             <1..185
                     /locus_tag="VFN42_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996393.1"
                     /translation="CLEGPMEVETRAPRAVHRLIPGQRCEVAPKTAHTVHGVDGGPCK
                     FLLMQGVGVYDNVAVG"
     gene            complement(825..1400)
                     /locus_tag="VFN42_06970"
     CDS             complement(825..1400)
                     /locus_tag="VFN42_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996394.1"
                     /translation="MSLDPNRLILTGENPFIRLGETDGGALTTNASFWRILISPAGAG
                     HVLYLKSELTQDRWKIYSDNIAMTRWLQKTVQGMLNGELSDTAIPVAEAAFDKAGDAR
                     DFWTERAVSHDEEVVLTWYDIGEPLLIHTQPAPPPGRKYGVCTVLIPALGARVTVNGV
                     QAKGRPFPAQREGKPFSTCALAFSESWTETR"
     gene            complement(1651..2433)
                     /locus_tag="VFN42_06975"
     CDS             complement(1651..2433)
                     /locus_tag="VFN42_06975"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HET8996395.1"
                     /translation="MQPAIFLSHGSPMLALTDTPARRFLTGLSASLPRPQAILVVSAH
                     WETAAPMLNAVARNETIHDFFGFPRPLYALRYPAPGDPALAQRIADLLGAAGFAAELD
                     RDRGLDHGAWVPLLLSWPAADIPVLQLSVQTALGPRHALAVGRALAPLREQGVLILGS
                     GSFTHDLRRFRGQPVNAPESADVAAFSCWMDAAIGRADTEAQLDYRARAPGARDEHPT
                     EEHILPLFTVMGAGGDGTARHLHHSTEHAILRMDAYGFGVAA"
     gene            complement(2433..2861)
                     /locus_tag="VFN42_06980"
     CDS             complement(2433..2861)
                     /locus_tag="VFN42_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET8996396.1"
                     /translation="MNSTIDTGTAGYGALLLRVTLGLLFLIHDGLKFFVFTPAGTEHY
                     FASLGLPGGFGIFVMLWELAAGIALILGIWPRLAALLIVPDLIGAIALVHIHSGFFFT
                     ATGGGWEYPAFWSIALIALALIGDGPYALLPTPFGQRRTA"
     gene            2959..>3521
                     /locus_tag="VFN42_06985"
     CDS             2959..>3521
                     /locus_tag="VFN42_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET8996397.1"
                     /translation="MDWDDLRSFLAIARAGSLTAAARRLGVQQSTMGRRLAALEAKAG
                     VRLLERTPGGFRLTAAGEAARVEVEKMEQAAMAAERAVSGRDVRLEGMVRLTTVSDFA
                     PSVLMPALADLAAGYPGIVVELIADDRTFSLAAREADLAIRLARPRGQSLLGRRIGEI
                     SFGLYASVEYLAAHSAAPLPGRGGEGHR"
BASE COUNT          586 a         1163 c         1228 g          544 t
ORIGIN      
        1 tctgcctgga aggaccgatg gaggtggaga cccgcgcgcc gcgcgccgtg caccgtctta
       61 tccccgggca gcgctgcgag gtcgcgccga agaccgcgca cacggtgcat ggggtggatg
      121 gcggcccctg caagttcctg ctgatgcagg gtgtcggtgt gtacgacaac gtggcggtcg
      181 gctaggcgac acgcagagat aggcggttcg gctggcggca ggctgctccg ttccgtcatg
      241 gtcggcgaag gcccatatgc gctaacttag gtgatcgggg cccgaacgtt ggcgccttgt
      301 ccggggtcga tccggccatt ctctccgtca acgagtgcac catgcgtgct gcacgctcgc
      361 cgggtcacat cctgagcgct cggcgcggta cgcggcaaag aaagcaagta caattaacac
      421 ggaggaaggg cgagggccac gggggccacg gagccgtgtc gccatgagga atcgagggac
      481 cgctccaaat cgcgccgcag gcagcatatt gttttcttct gccagcgggt ccgtttgccg
      541 caacgaagca gaccaggaaa tccgctccgt gatccccgtg gccctcgccc ttcctccgtg
      601 ttaaagatac tgtcctgctt gcatgccacc acgccagact gggactcgcc agactgcctc
      661 gtataagttg gcgcgtatgg cgaaggctga ccgtccacga cttctccgaa cggccgcagc
      721 acagtcgcgg atggtcggcc ttcgccgacc atgacgaggt ggcataagcc ggccggtgtg
      781 cagtggtgat tcctgcgcgc cgcctaacgc cggaacgcgc cggttcagcg ggtctcggtc
      841 cagctctcgg agaatgccag ggcgcaggtg ctgaacggct tgccctcgcg ctgcgccggg
      901 aacggccggc ccttcgcctg gacgccattg accgtcacgc gtgccccgag cgccggaatc
      961 agcaccgtgc acacgccgta tttgcgtccc ggcggaggag ctggctgggt gtggatcagc
     1021 agcggctcac cgatatcgta ccaggtcaac acgacctcct cgtcatgcga caccgcccgt
     1081 tcggtccaga agtcccgcgc atcgcccgct ttgtcgaacg ccgcctcggc cactggaatc
     1141 gcggtatccg acagctcacc gttgagcatg ccctgcacgg ttttctgcag ccagcgcgtc
     1201 atggcgatgt tgtcggagta gatcttccac cggtcctgcg tcagctcgct cttcaggtaa
     1261 agcacgtggc cggcgccggc gggcgaaatc aggatgcgcc agaaactcgc gttggtggtg
     1321 agcgcgccgc catccgtttc acccaggcgg atgaacgggt tttcgccggt caggatcagc
     1381 cggttcggat caagggacat cgggatttcc tctgttggcg cccgatgaac ttacaccagc
     1441 ccgcgctgac gccaactctc tggtgtcatg gccggcggca ggccggctac ccacgtcttc
     1501 tctcgggcac acacggaaca gacgtgggtg cccgcgccaa gcgcgggcat ggcacatggg
     1561 tgaaggctta cgcggcccgg tattagcggt catgcggtgc cgctcaggcg gtccgcgtcc
     1621 cctccccgct cgtcccggct ttccgccggg tcaggccgca acgccgaacc cgtaggcatc
     1681 catccgcagg atcgcgtgct cggtcgagtg gtggaggtgc cgcgccgtgc catcgccccc
     1741 cgcccccatc acggtgaaga gcggcaggat atgctcctcg gtcggatgct cgtcgcgcgc
     1801 accgggagcc cgggcgcggt agtccagctg cgcctcagtg tcggcccgcc ctatcgccgc
     1861 gtccatccag caactgaacg cggcgacgtc tgcgctttcc ggtgcattca ccggttggcc
     1921 gcggaagcgc cgcagatcgt gggtgaagct ccccgaaccg aggatcagca cgccctgctc
     1981 gcgcagcggc gccagggcgc ggccgaccgc caaagcgtgg cgcggtccca gcgcggtctg
     2041 cacggacagc tgcagcaccg ggatatcggc cgcgggccag gacagcagca acggcaccca
     2101 ggcgccgtga tccagaccgc ggtctcggtc caactcggcc gcaaacccgg cggcgccgag
     2161 cagatccgcg atccgctgcg ccagcgccgg atcgcccggc gccggatagc gaagcgcgta
     2221 cagcgggcgc ggaaagccga agaaatcgtg gatggtctcg ttccgcgcca ccgcgttgag
     2281 catcggcgcg gcggtttccc agtgcgcgga caccaccagg atcgcctgcg ggcgcggcag
     2341 gctggccgac agcccggtga ggaaacgccg cgccggggta tcggtcagcg ccagcatggg
     2401 cgagccgtgg ctgaggaaga tggctggctg catcatgccg tcctcctctg gccgaacggg
     2461 gtcggcagca gggcgtaggg gccgtcgccg atcaacgcca aggcgatcag cgcgatgctc
     2521 cagaaggcgg gatattccca gccgccaccc gtcgcggtga agaagaagcc gctgtggatg
     2581 tgcaccaggg cgatggcgcc gatcaggtcg ggcacgatca gcagggcggc gaggcgcggc
     2641 cagatgccca ggatgagcgc gatgccggcg gcaagctccc acagcatgac gaagatgccg
     2701 aacccgcccg gcaaacccag gctggcgaag taatgttccg tgccggccgg ggtgaacacg
     2761 aagaacttca gcccgtcatg gatgaggaac agcaacccga gcgtcacccg caacaacagg
     2821 gcgccgtagc cggcagtgcc ggtatcgatc gtggaattca tcggcagtct cctttccgca
     2881 aagagatagc catgcggcag acgctggaac attggcgaat tccgccttat cgtcatgcgt
     2941 tttcgcatga aggcccggat ggactgggac gacctcagaa gcttcctcgc gatcgcgcgt
     3001 gcaggcagcc tgacggcggc ggcgcggcgc ctgggcgtgc agcaatcgac catgggccgg
     3061 cggctggcgg cactggaagc gaaggctggc gtccggctgc tggagcgcac gccgggcggc
     3121 ttccggttga ccgcggccgg cgaggccgcg cgcgtcgagg tggaaaagat ggagcaggcg
     3181 gcgatggccg ccgaacgcgc ggtcagtggg cgcgacgtgc ggctggaggg catggtgcgg
     3241 ctgaccacgg tgagcgactt cgcgccgtcc gtgctgatgc cggcgctggc cgacctggcg
     3301 gcgggttacc cgggtatcgt ggtcgaactg atcgccgatg accgcacctt ctccctggcc
     3361 gcccgggagg cggacctggc gatccgcctg gccaggccgc gcgggcaatc gctgctgggg
     3421 cggcggatcg gcgaaatctc attcggcctg tacgcgagcg tggagtacct ggcagcgcac
     3481 agcgctgcgc cgttgccggg acgtggcggc gaaggccacc g
//
LOCUS       DASSZH010000326         3744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_979330, whole genome shotgun sequence.
ACCESSION   DASSZH010000326 DASSZH010000000
VERSION     DASSZH010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979330"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFN42_06990"
     CDS             complement(<1..462)
                     /locus_tag="VFN42_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HET8996398.1"
                     /translation="MDGDLSRLNAFGLRALPGAAFGEDPLAARPLDPGMGLAVARRTV
                     FRPEDQECFGRVADRVAAGNIALLGRHDPVTAIEQARLRNAIATGALLTSGRHLQHGD
                     LDQPTRNMEVFTNCATAACSFTKFYLLLNGSGVGRSYDDALMAVAGSWRPSR"
     gene            complement(570..965)
                     /locus_tag="VFN42_06995"
     CDS             complement(570..965)
                     /locus_tag="VFN42_06995"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HET8996399.1"
                     /translation="MVRSTADRLADILDAATDLRDFVADMATDAFHRLPHADRMGYRA
                     IKNALTELGEAAKAVPQQVKNRHPEVDWKGFAGLRDLMAHRYFGIETSSLLPIIRNEI
                     PALLAAVEAELRAGDDADSGPVHSGPDRD"
     gene            complement(965..1408)
                     /locus_tag="VFN42_07000"
     CDS             complement(965..1408)
                     /locus_tag="VFN42_07000"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET8996400.1"
                     /translation="MGDTAFLSVRVPQHFRDRMKAIAAQRGENLQDLIGNLIARFLED
                     AERRPPELAHVVRRLRALEGLLRARHVAALWVFGSVARGDARQDSDVDLALEFAEGRQ
                     PSLLEIAHIKELVETGIGHRADIGERRLLNRRVAATAERDMVRVF"
     gene            complement(1536..2201)
                     /locus_tag="VFN42_07005"
     CDS             complement(1536..2201)
                     /locus_tag="VFN42_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003617827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8996401.1"
                     /translation="MPEVMFAGPDGRLEGRYHHSKESGAPVALILHPHPLHGGTMNNR
                     ITYSMYQAFQRLGCSVMRFNFRGVGRSQGRYDGGIGEIGDAAAALDWMQAVNPNSGGL
                     WIAGYSFGAFIGMQLLMRRPEISGWVSVAPPANHYDFGFLAPCPCGGLMIHGDADELV
                     PEPAVRKLVDKLNTQKGVHVDYRVFAGADHVFANHADTIAEAVEDHCGKAITRRQMAL
                     AAD"
     gene            2558..3247
                     /gene="cysE"
                     /locus_tag="VFN42_07010"
     CDS             2558..3247
                     /gene="cysE"
                     /locus_tag="VFN42_07010"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HET8996402.1"
                     /translation="MAFMFLRETIRTYRERDPAARSGLEVLLCYPGVHAAMWHRLSHW
                     LWTNQFYLAGRFSSHIARWLTGIEIHPAARLGRRLVIDHGMGVVIGETAEIGDDCYLY
                     HQVTLGVARTMGGKRHPTVGNNVIIGAGAKVLGPITVGDNARIGSNAVVVDPVPADTT
                     VVGIPARPVDRKLPRKAPHFDPYGMPCDDSLDPLLRDLEGMRHELSELEARVARLAML
                     EQAQQREAAGE"
     gene            3250..>3744
                     /locus_tag="VFN42_07015"
     CDS             3250..>3744
                     /locus_tag="VFN42_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET8996403.1"
                     /translation="MQLSTRGRYAVMAMVDLATRQAIGCECGPVCLAEIAGRQQLSLA
                     YLEQLFGKLRRAGLVASARGPGGGYRLARASDTITVADIVDAVQEPIHATRCETGTGG
                     CMAGPVPGAAGERCQTHDLWHELGRQIALFLQGVTLADVVSQRVRGRAVPVEATEPAA
                     APLAA"
BASE COUNT          615 a         1241 c         1264 g          624 t
ORIGIN      
        1 cctgctcggc cgccacgatc ccgccaccgc catcaacgcg tcgtcgtagc tgcggccgac
       61 gccggagccg ttcaacagca ggtagaactt ggtgaaggag caggccgccg tcgcgcagtt
      121 ggtgaacacc tccatattgc gggtcggctg gtcgaggtcg ccgtgctgga gatgccggcc
      181 ggaggtcagc agcgcaccgg tggcgatggc attgcgcagg cgggcctgct cgatggcggt
      241 caccgggtcg tggcggccga gcagggcgat gttgccggcg gcgacgcggt cggcgacgcg
      301 gccgaagcac tcctggtctt ccgggcggaa gacggtgcgg cgggccacgg ccagacccat
      361 gccggggtcc agcggacggg ccgccagggg gtcctcgccg aacgccgcgc cgggaagggc
      421 acgcagtccg aaggcgttca gccgggaaag atcgccgtcc atcgtcatct gccctgccgg
      481 ttgccgtgcc acaagatata gtcgattccc ggcgctatgg caaccatata ccgggtgcag
      541 cggggcctcg cagcaaggct tttgctttcc tagtcccgat ccggtccact gtggacgggg
      601 cctgaatccg catcgtctcc ggcgcgcagt tccgcttcga cggcggcgag cagggcgggg
      661 atttcgttgc ggatgatcgg cagcaggctg ctggtctcaa tcccgaaata gcggtgcgcc
      721 atcaagtccc gcagaccggc gaagcccttc cagtccacct ccggatgccg gttcttcacc
      781 tgttgcggaa cggcctttgc ggcctcgcca agctcggtca aggcgttctt gatggcgcgg
      841 taccccatgc ggtcggcgtg cggcagcctg tggaaggcgt cggtcgccat gtcggcaacg
      901 aaatcgcgca ggtcggtcgc ggcatccagg atgtcggcga ggcggtccgc cgtagaacgt
      961 accatcagaa gacgcgcacc atgtcgcgtt cagccgttgc ggcaacgcgc cggttcagca
     1021 aacggcgttc cccgatgtcg gcgcgatgcc cgatgcccgt ttcaacgagt tccttgatgt
     1081 gcgcgatctc cagcaaggag ggttggcggc cctccgcaaa ctcgagcgcg agatccacgt
     1141 cgctgtcctg cctggcgtca ccacgcgcaa ctgagccgaa cacccacaaa gcggcgacat
     1201 ggcgcgcacg caggagccct tcgagcgcgc gcaaccggcg aacgacatga gcaagttccg
     1261 gtgggcgccg ttcggcatcc tccaggaacc gagcaatcag gttgccgatc agatcctgca
     1321 ggttttctcc tcgctgcgcc gcgatcgcct tcatgcggtc gcggaaatgc tgcgggacgc
     1381 gcacggacag gaaggctgta tcgcccacgt tcactcctcg gctgcgctgc taacttgtgc
     1441 acaaatagga cggcgcatca aaatcgtcca ccaaaaccaa gggcggcgga cccggaggtc
     1501 cgccgccctt gaaacgcatt gactgcggtg ccggctcagt cggcggcgag tgccatctgg
     1561 cggcgggtga tcgccttgcc gcaatggtct tccaccgcct cggcgatggt gtcggcatgg
     1621 ttggcaaaga cgtggtcggc gccggcgaac acacggtagt cgacgtgcac gcccttctgg
     1681 gtgttcagct tgtccaccag cttccgtacg gccggctccg gcaccagctc gtcggcgtcg
     1741 ccgtggatca tcagcccgcc gcaggggcag ggggccagga agccgaaatc gtagtggttg
     1801 gcgggcggcg cgacgctgac ccagccggag atttccggcc ggcgcatcag aagctgcatg
     1861 ccgatgaacg cgccgaacga gtagccggcg atccacagtc caccggagtt cgggttcacc
     1921 gcctgcatcc agtcaagcgc cgccgcggca tccccgatct cgccgatgcc gccgtcatag
     1981 cggccctggc tgcgtcccac gccacggaaa ttgaaccgca tcaccgagca gcccaggcgc
     2041 tggaacgcct ggtacataga gtaggtgatt cgattgttca tcgtcccgcc atgcagcggg
     2101 tgcgggtgca gaatcagcgc cacgggggca ccggactcct tcgagtggtg ataacggccc
     2161 tcaagccggc catccggccc ggcaaacatc acttccggca ttggcgatcc cgaaacctgt
     2221 tcctgtcgca tccggcgtca cgccggagca ggcccccatt tgccctgctc caccgccggt
     2281 ccaaaaacac cacgcgaccc attctcccct gggctaggcc tcatagccga acatcagata
     2341 ggaacgatga ccccgtaagt cagttccgcg tgaagccttc cccggtcaat tcttgacccg
     2401 tggagtcgga aattctagct tcaacgccgg ctgtccgctg acggggttcc ctcctgtctc
     2461 atggccggcc cagggccttt gctgcggaag cgggccttcc tccgcggcgg acgcactata
     2521 cgggatgcaa ccccaacatt cataacggaa ttgtcgcatg gctttcatgt tcctgaggga
     2581 gacgatccga acgtatcgcg aacgcgatcc ggcggcacga tccgggctgg aggttttgct
     2641 ttgctaccca ggcgtgcatg cagcgatgtg gcatcgcctg tcccactggt tgtggaccaa
     2701 tcaattctac ttggctgggc ggttctcgtc ccatattgcg cggtggctca ccggcatcga
     2761 gattcatccc gccgcccggt tggggcgtcg cctggtgatc gaccacggca tgggcgtggt
     2821 gatcggcgag accgccgaaa tcggcgacga ttgctacctc tatcatcaag tcaccctggg
     2881 ggtcgcccgc accatgggcg gcaaacgcca ccccaccgtg ggcaacaatg tcatcatcgg
     2941 cgccggcgcc aaggtgctgg ggccgatcac cgtcggcgat aacgcccgca tcggctccaa
     3001 tgcggtggtg gtcgatccgg tccccgccga taccaccgtc gtgggcattc ccgcgcgtcc
     3061 ggtggaccgc aagctgccgc gcaaggcccc gcatttcgac ccctatggca tgccatgcga
     3121 cgacagtctg gatccgttgc tgcgcgacct ggagggcatg cgccacgaac tgtccgagct
     3181 ggaggcgcgg gtggcgcgcc tggcgatgct cgagcaggcg cagcagcgcg aggcggcggg
     3241 ggaatagcca tgcaactctc cacccgtggg cgctatgcgg tgatggcgat ggtcgacctg
     3301 gcgacgcggc aagcgatcgg ctgcgagtgc ggtcccgtct gcctggccga gatcgccggc
     3361 cggcagcagc tcagcctggc ctatctggag cagctgttcg gcaagttgcg gcgggccggg
     3421 ctggtggctt cggcgcgtgg gccgggcggc ggctaccgcc tggcgcgcgc gtcggacacg
     3481 atcaccgtgg cggacatcgt ggacgcggtg caggagccga tccatgccac gcgctgcgaa
     3541 acgggaaccg gcggctgcat ggctggcccg gtgccgggcg cggcgggcga gcggtgccag
     3601 acgcacgatc tgtggcacga gctgggccgg cagatcgcct tgttcctcca gggcgtcaca
     3661 ctggccgatg tggtgtcgca gcgggtgcgc gggcgcgccg tgccggtgga ggcgacggaa
     3721 cccgcggcag cgccgctggc ggcg
//
LOCUS       DASSZH010000327        10090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1099015, whole genome shotgun sequence.
ACCESSION   DASSZH010000327 DASSZH010000000
VERSION     DASSZH010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 10090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10090
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099015"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..479
                     /locus_tag="VFN42_07020"
     CDS             174..479
                     /locus_tag="VFN42_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996404.1"
                     /translation="MNAVPFDTLKLARDLEAAGLAAPVAAGTAAALADAMVGADLATR
                     SDVGTAESRIMAAIRAVENSLDSRLELLRRDMTIKLGGMIFIAVGIILTAMRFMPHP"
     gene            complement(491..730)
                     /locus_tag="VFN42_07025"
                     /pseudo
     CDS             complement(491..730)
                     /locus_tag="VFN42_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014104984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
     gene            841..1113
                     /locus_tag="VFN42_07030"
     CDS             841..1113
                     /locus_tag="VFN42_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996405.1"
                     /translation="MNAVPFDTLKLARDLEAAGLAAPMAAGTAAALADAMVGADLATR
                     SDLALTEGRVMGALELLRRDMTIKLGGMIFIAVGIILTAMRFMPHP"
     gene            complement(1125..1763)
                     /gene="upp"
                     /locus_tag="VFN42_07035"
     CDS             complement(1125..1763)
                     /gene="upp"
                     /locus_tag="VFN42_07035"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HET8996406.1"
                     /translation="MSYPANVTVVDHPLVQHKLTLLRRKHTATAEFRRLVREISTLMA
                     YEVTRDLPTESVAIETPLEPTDAPLLSGKKLCLLSILRAGNGMLDGMLDLVPSARVAH
                     IGLYRDPHTLIPVEYYLKLPDDIHERLVIVVDPMLATGHSAIAALHRAKQAGATQIRF
                     ACLLAAPEGVQAFTESHPDVRLYIGAIDRQLDEHGYIRPGLGDAGDRLYGTR"
     gene            complement(1786..3003)
                     /locus_tag="VFN42_07040"
     CDS             complement(1786..3003)
                     /locus_tag="VFN42_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="URC4/urg3 family protein"
                     /protein_id="HET8996407.1"
                     /translation="MRRAVAWLRTPEAVRQRCHALLALAEQDRLPHFTLHADQLDEVA
                     DFVAQVTRENYPDLAIPYHSRWRHFEIGGIDRWSVLADSVAGPAGERARVRLELCTIS
                     VLLDAGAGPDWRFHERETGQSWGRSEGLAIASIAAFRAGLFSGDPRQALRVDAEGLSR
                     ITEARLAHALQVAAANPLTGLAGRAALLRSLGAALRRQPQLFGPEARLGRLFDAWRDH
                     AAERALSARDILLTLLECFATIWPRRPTLGGENLGDVWPHAALDSPEPGAALVPFHKL
                     SQWLCYSLVEALEQSAIPVQELDALTGLAEYRNGGLFIDGGVLQLRDPVLANTPLPPE
                     HAAVVEWRALTVALLDRLAPLLRHRLGRSERELPLACVLQGGTWDAGRQLARERRTDG
                     SPPIAVLSDGSLF"
     gene            complement(3024..4301)
                     /locus_tag="VFN42_07045"
     CDS             complement(3024..4301)
                     /locus_tag="VFN42_07045"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HET8996408.1"
                     /translation="MPGVSVPPAPRGTVPQAILLTSHPRPGQGRHIRIRWGAETPQER
                     GPVIATLHEPDARNAIGSHAGSYAVYRALAIAAGQLAAGHRPDLTNTTPAERIGPFPQ
                     WSGSDRIVSLDPWGHLTSEAFAEHLAAGRDIRPTIAVTRARINMPEVVQAMRDGRLTP
                     DGDLLTKAGDVRVTKVAIDPVWYLPGIAQRFGITTTALRRCLFEQTAGMFPELVTRPD
                     LDVFLPPIGGMTVYLFGDVSRLGRPETKIACRVHDECNGSDVFGSDICTCRPYLAHGV
                     EVCIATGQQGGLGIVVYNRKEGRALGEVTKFLVYNARKRQEGGDRAEAYFHRTECIAG
                     VPDMRFQELMPDVLHWLGIARIDRLVSMSNMKYAPIVASGIAVVERVPIPDALIPADA
                     AVEMDAKKAAGYYTDMVPDAWELKLAKGRGLAD"
     gene            complement(4369..4713)
                     /locus_tag="VFN42_07050"
     CDS             complement(4369..4713)
                     /locus_tag="VFN42_07050"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET8996409.1"
                     /translation="MTAALDLSDADLAILRAVLEQHLPNGARVFVFGSRAHGGARQYS
                     DLDLALAWDQPLGLELIGSIAEALSESDLPYKVDLVDLSTVDPSFRDRIARQCVALPL
                     DTGSVITPARNL"
     gene            complement(4710..5159)
                     /locus_tag="VFN42_07055"
     CDS             complement(4710..5159)
                     /locus_tag="VFN42_07055"
                     /inference="COORDINATES: protein motif:HMM:NF020360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HI0074 family nucleotidyltransferase
                     substrate-binding subunit"
                     /protein_id="HET8996410.1"
                     /translation="MAAQDGSGPDDRAGSAGLEITPLVSAVARLREGLDRHLREPADE
                     QLRDGLIQRFEFTYELAHRLLRRYLREVSASPASLDQMTFADLIRSGNEQGLLRGDWP
                     AWRRYRDMRARTSHTYDVTVAAQVVAGIPDFLAEAVYLRDQLQRRLT"
     gene            complement(5190..6326)
                     /gene="ispG"
                     /locus_tag="VFN42_07060"
                     /gene_synonym="gcpE"
     CDS             complement(5190..6326)
                     /gene="ispG"
                     /locus_tag="VFN42_07060"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010507870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HET8996411.1"
                     /translation="MMSYRPYQEIIRRKSRRVYVGNVPVGDGAPISVQTMTNTLTTDV
                     EGTVAQIRRAELAGVDIVRVSCPDRESALALKDIVHEVNVPIVADIHFHYKRAIEAAE
                     SGAACLRINPGNIGSDERVKEVVKAARDHGCSMRIGVNAGSLEKHLLEKYGEPNPEAL
                     VESALYHANILQQHDFHDFKISVKASDVFLAVAAYQQLAEVCDHPLHIGITEAGGRRM
                     GTVKSSIGLGSLLWAGIGDTMRVSLSDEPEEEVRVAWDMLKTLGLRHRGVKVISCPSC
                     ARQGFNVIQTVQTLEDRLAHIETPITLSIIGCVVNGPGEALMTDLGVTGGGNGRHMIY
                     SAGKTDHTIPGGDMVEHIVELVEAKAAELRAAQAAAEPEKVAAE"
     gene            complement(6400..7494)
                     /locus_tag="VFN42_07065"
     CDS             complement(6400..7494)
                     /locus_tag="VFN42_07065"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HET8996412.1"
                     /translation="MMSPVGPEQDEPPLAARAGADLRAARERLGWDLSEIAAGLRIRQ
                     PHLEALEEGRIDLLPAHAYALGFLRTYAASLGLDPEEIVRRFKAEAGRVNTRTELAFP
                     APMPERGIPAGAVVLLGLVLAIGAYAGWYRLSGEGRLPPESIAIPQHLQTLAQQAMPP
                     VAKLPRTSVAAATPAQDPSPTSAEAAEPPAQVVSPTSAAAASVTPPLKQPDAAQAGAS
                     PQNPGATAAQPPPQTVLAGQLVAAQPQVPAAADSAAASTPQQPQVVLRATADSWVQVR
                     DKSGQVLLNRILHAGDSWQVPDRPNLLLTTGNAGGTELELNGVSTGLLGAPGAVRRDL
                     PLDPALIKQGALNPPSGNGVQQASLRTPSR"
     gene            complement(7805..10048)
                     /gene="ptsP"
                     /locus_tag="VFN42_07070"
     CDS             complement(7805..10048)
                     /gene="ptsP"
                     /locus_tag="VFN42_07070"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET8996413.1"
                     /translation="MPGTRSLLVRLRDLRASGSAPLGELVRLVASELVAEVCSIYAQK
                     PGDILELVATVGLNPDAIGHTRLRVGEGIVGLCAATGAVMNLPDAQNHPAFAYRPETG
                     EEPYASMLAVPVRRAGRTLGVIAAQNRNPRHYTEEEVDEMETVAMLVAELLPASGATD
                     GAPEGLAATVPRVFAGSSLTAGIAIGPVVLHGMPRVQVPLLAEDPMAELTRLHGASER
                     MQRGLDELIKGNALDGGGDADAHASREVLDAYRLVASDAGWMHRVEEVIRTGVSAEAA
                     VQRVAGDLHDRMRRVTDPYLRERLADIEDLAGRLLSALIGDVPRPEVPPGSILLARRL
                     GPAELLDWHAQGIAGIAIEEGSPAGHAAILARALGIPAVGGVRGMLDTAEQGDEAIID
                     ADEGQLVLRPEADVREVYARALENQTTLQAGYATLRDKPGCTADGTRVELMLNAGLAL
                     ELAQLDSTGADGIGLFRTEIAMMARGSVADVAEQAALYARVLDAAGDRPVVFRTLDLG
                     ADKMLPGTISEEENPAMGWRSLRIGLDRPALLRRQLRALLLAAGGRPLTVMFPMVATV
                     TEFRAARALLQAEARRVRPAPDRLSIGTMLEVPALMWQLPELLKEVDFVSVGSNDLLQ
                     FLFAADRGTPSLYARYDLLSQPVFDLLDQLLTAARAARNGAGVPLSICGEAASRPLEA
                     LALVGLGLTNLSMPAAGILPVKALLAQLDLTAFRPVLASIRRGGAGTASLREPIATWA
                     REHGLPA"
BASE COUNT         1539 a         3492 c         3502 g         1557 t
ORIGIN      
        1 accctggtgt tcgaccagga ccacgatacc aagtggcggc gggcgctggc caagctgaag
       61 atcgatcccc tgctgctgtc ggagtcggcc gggcacgcct gaggcctccg gcaacgttgc
      121 cttaagcctc ctgtgatgga ctgcggcgga acccgtgccg taggatcatg gccatgaacg
      181 ctgtcccgtt cgacacattg aagctggcgc gcgacctgga ggcggccggt ctggctgccc
      241 cggtggcggc cggaaccgca gctgccctgg ccgacgcgat ggtcggcgcc gatctggcga
      301 ccaggtcgga tgtgggtacc gcggagtcgc ggatcatggc ggcgatccgt gcggtggaga
      361 attccctgga ctcccggctg gaattgctgc gccgggacat gaccatcaag ctgggcggca
      421 tgatctttat cgccgtcggg atcatcctga cggcgatgcg gttcatgccg catccctgaa
      481 ccggcgcggc tcagcgcgtt ccgtacagcc ggtcgccggc gtcgcccagg ccggggcgga
      541 tatagccatg atcgtccaac tggcgatcga tcgcgccatt gtacaggcgg acatccggat
      601 ggctttcggt gaaggcttgc acgccgtccg gtggtgccag caggcaggaa aagcggatct
      661 gcgtcgcgcc cgcctgcttc gcccggcgca gcgccgcgtt ggcggagtgg ccggtggcca
      721 gcatcggatc gagccgctgc cgctatggga gtcggccggg cacgcctgag gcctccggca
      781 acgttgcctt aagcctcctg tgatggactg cggcggaacc cgtgccgtag gatcatggcc
      841 atgaacgctg tcccgttcga taccctgaag ctggcgcgcg acctggaggc ggccggcttg
      901 gccgccccga tggcggccgg aaccgcagcc gccctggccg acgcgatggt cggcgccgat
      961 ctggcgacca ggtccgatct cgctcttacc gaaggccggg tgatgggcgc gctggaactg
     1021 ctgcgccggg acatgaccat caagctgggc ggcatgatct ttatcgccgt cgggatcatc
     1081 ctgacggcga tgcggttcat gccgcatccc tgaaccggcg cggttcagcg cgttccgtac
     1141 agccggtcac cggcgtcgcc caggccgggg cggatgtagc catgctcgtc caactggcgg
     1201 tcgatcgcgc caatgtacag gcggacatcc ggatggcttt cggtgaaggc ctgcacgccc
     1261 tccggtgccg ccagcaggca ggcgaagcgg atctgcgtcg cgcccgcctg cttcgcccgg
     1321 tgcagcgccg cgatggccga atggccggtg gcgagcatcg gatcgaccac gatcaccagc
     1381 cgctcgtgga tgtcgtccgg cagtttcaga tagtactcga ccggaatcag cgtgtgcgga
     1441 tcgcgataca gcccgatatg cgcgacccgc gccgagggca ccagatcgag catgccgtcc
     1501 agcatgccgt tgccggcgcg caggatcgac agcaggcaca gcttcttgcc ggatagcagt
     1561 ggcgcatcgg tgggctccag cggcgtttcg atcgccacgc tctcggtggg caggtcgcgc
     1621 gttacttcgt aggccatcag cgtgctgatc tcgcggacca ggcggcggaa ttcggcggtg
     1681 gcggtgtgct tgcgccggag cagcgtcagc ttgtgctgca ccagcgggtg atccacgacg
     1741 gtgacgttgg ccgggtacga catccgcaag gtctttccac tcgggtcaaa acaggctgcc
     1801 gtcactcagc acggcgatcg gtgggctgcc gtcggtccgc cgttcccgcg ccagctggcg
     1861 gccggcgtcc caggtgccgc cttgcagcac gcaggccagc ggcagctcac gttccgagcg
     1921 gcccagccga tggcgcagca gcggtgccag gcggtccagc agcgcgaccg tcagtgcgcg
     1981 ccattccacc acggcggcat gctccggtgg aagcggggtg ttcgccagca ccggatcgcg
     2041 cagctggagg acgccaccgt cgatgaacag gccgccgttg cggtattctg cgaggccggt
     2101 cagcgcatcc agctcctgca ccggaatcgc cgactgctcc agcgcctcca ccagcgagta
     2161 gcacagccat tgcgacagct tgtggaacgg caccagggcc gcaccgggtt ccggtgagtc
     2221 gagcgcggca tgcggccaga catcgcccag gttctcgcca cccagcgtcg gtcgtcgcgg
     2281 ccagatggtg gcgaagcact caagcagcgt cagcaggatg tcccgtgcgg aaagggcgcg
     2341 ctccgcggca tggtcgcgcc acgcatcgaa caaccggccc agcctcgcct ccgggccgaa
     2401 cagctgcggc tgccggcgta acgcggcacc gaggctgcgc agcaaggcgg cgcggcctgc
     2461 cagtccggtc aacgggttgg ctgcggcaac ctgcaacgcg tgagcgagac gcgcttcggt
     2521 gatgcgtgaa agtccttcgg catcaacgcg caaggcctgc cggggatcgc cggagaacag
     2581 gccggcgcgg aatgcggcga tgctggcgat cgccagacct tccgagcggc cccagctctg
     2641 gccggtctcc cgttcgtgga agcgccagtc aggaccggcg ccggcatcga gcagcacgct
     2701 gatggtgcaa agctccaacc ggacgcgggc gcgttcgccg gcaggaccgg cgacggagtc
     2761 cgccagcacg ctccagcggt cgatcccgcc aatttcgaaa tgccgccagc gcgaatgata
     2821 ggggatcgcc aggtcgggat aattctcgcg cgtcacctgt gcgacgaaat ccgccacttc
     2881 gtccagctga tccgcgtgca gcgtgaaatg cggcagccgg tcctgctctg ccaatgcgag
     2941 cagggcatgg cagcgctggc gaacggcttc cggcgtgcgc agccaggcca ccgcgcgccg
     3001 cactgactca tgctggtcgg tgctcaatcg gccaggccgc ggcctttggc cagcttcagt
     3061 tcccacgcat ccggcaccat gtcggtgtag tagccggcgg ccttcttcgc atccatttcc
     3121 acggcggcgt cggcggggat cagcgcgtcg gggatcggca cgcgttccac caccgcgatg
     3181 ccgctggcga cgatcggggc gtatttcatg ttgctcatcg acaccaggcg gtcgatccgt
     3241 gcgatgccca accagtgcag cacgtcgggc atcagttcct ggaagcgcat gtcgggcaca
     3301 ccggcgatgc attccgtgcg gtggaaatag gcctccgcgc ggtcgccgcc ttcctgccgc
     3361 ttgcgcgcgt tatagaccag gaacttggtg acctcgccga gtgccctgcc ttccttgcgg
     3421 ttgtacacga cgatgccgag gccaccctgc tggccggtgg cgatgcagac ttccacgcca
     3481 tgtgccaggt acgggcggca ggtgcagatg tcggaaccga acacgtcgga gccgttgcat
     3541 tcgtcgtgca cgcggcaggc gattttggtt tccggccgcc cgaggcggga aacgtcgccg
     3601 aacagataga cggtcatgcc gccgatcggc ggcaggaaca cgtccaggtc gggccgggtc
     3661 accagttcgg ggaacatccc ggcggtctgc tcgaacaggc agcggcgcaa cgcggtggtg
     3721 gtgatgccga agcgttgcgc gatgcccggc agataccaga ccgggtcgat cgccaccttg
     3781 gtcacgcgca cgtcgccggc cttggtcagc aggtcgccgt ccggtgtcag gcgtccgtcg
     3841 cgcatcgcct gcacgacctc cggcatgttg atgcgggctc gcgtcaccgc gatggtgggg
     3901 cggatgtcgc gtccggcagc gaggtgttcg gcgaacgctt cgctcgtcag gtggccccag
     3961 ggatcgagcg acacgatgcg gtcgctgccg ctccattgcg ggaaggggcc gatgcgttcc
     4021 gccggcgtgg tgttggtgag atcgggacgg tgccccgcgg cgagctgtcc ggcggcgatc
     4081 gccagcgcac gatacaccgc ataggacccg gcatggctgc cgatggcgtt gcgtgcgtcg
     4141 ggttcgtgca gggtggcaat gaccgggccg cgctcctgcg gcgtctcggc gccccagcgg
     4201 atccggatat gacgcccctg gccggggcga ggatgcgacg tcagcaggat ggcctgcggc
     4261 acggtgccgc gaggtgcagg cgggaccgaa acgccgggca ttgttgtcac gctcccgtgg
     4321 ggcaggacag gatcataatg ccagcgtagg gaactcctct gcggcggtct acagattgcg
     4381 cgcaggcgtg atcacgctcc cggtgtcgag aggcagggcg acgcactggc gggcgatgcg
     4441 gtccctgaac gacggatcga ccgtcgagag atcgacgaga tcgaccttgt acggcagatc
     4501 cgattccgac agcgcctcgg cgatcgaccc gatcagctcc agcccgagcg gctggtccca
     4561 ggccagcgcc aagtcgagat cggagtattg ccgggccccg ccatgggcgc gcgagccaaa
     4621 cacgaaaacc cgcgctccgt tgggcaggtg ctgctccagc acggcacgca ggatcgccag
     4681 atccgcatcg ctgaggtcca gggcggccgt catgtcagcc gcctctgcaa ctggtcacgg
     4741 aggtacacgg cttccgccag gaaatccgga attccggcca cgacctgggc cgcaaccgtc
     4801 acgtcatagg tgtgactcgt ccgcgcacgc atatcgcgat agcgtcgcca ggccggccaa
     4861 tcgccccgca gcaaaccctg ctcgttccct gaccggatca ggtccgcgaa ggtcatctga
     4921 tcgagcgacg cgggagaggc ggatacctcc cggaggtagc ggcgcagcag gcggtgcgcc
     4981 agctcatagg tgaattcgaa ccgctgaatc aggccatcgc gcagctgctc atccgcgggt
     5041 tcgcggagat gccggtccaa tccttcccgc agtcgcgcca ccgcgctgac cagcggcgtg
     5101 atttcgagcc ccgcgctgcc cgcccggtcg tccggtccgg agccgtcttg cgcagccagc
     5161 gcagtgtcct ccgtactccg cggcgcaacc tattccgccg ccaccttctc cggttccgcc
     5221 gccgcctgcg ccgcccgcag ctctgcggcc ttcgcctcca ccagctcgac gatgtgctcc
     5281 accatgtcgc cgccggggat ggtgtggtcg gtcttgccgg cgctgtagat catgtgccgg
     5341 ccgttgccgc cgccagtcac gccgaggtcg gtcatcagcg cttcgcccgg gccgttcacc
     5401 acgcagccga tgatcgacag cgtgatcggc gtctcgatat gcgccagccg gtcctccagc
     5461 gtctgcaccg tctggatcac gttgaacccc tggcgcgcgc aggacggaca ggagatcacc
     5521 ttcaccccgc gatgccgcag gcccagcgtc ttcagcatgt cccaggcgac gcggacttcc
     5581 tcctccggct catcggacag cgagactcgc atggtgtcgc cgatgccggc ccacagcagt
     5641 gaacccagcc cgatcgacga cttcaccgtg cccatgcgcc ggccgccggc ctcggtgatg
     5701 ccgatatgca gcgggtgatc gcagacttcc gcgagctgct ggtaggcggc caccgccagg
     5761 aacacgtcgg aagccttcac gctgatcttg aagtcgtgga aatcgtgctg ctgcaggatg
     5821 ttggcgtggt acagcgcgct ctcgaccagg gcctccgggt tcggctcgcc gtatttctcc
     5881 agcaggtgct tctccagcga cccggcatta accccgatcc gcatcgagca gccgtgatcg
     5941 cgggcggcct ttaccacctc cttcacgcgc tcatcgctgc cgatattgcc cgggttgatg
     6001 cgcaggcagg cggcgccact ctcggccgct tcgatcgcgc gcttgtagtg gaagtggatg
     6061 tcggcgacga tcggcacgtt gacctcgtgc acgatgtcct tcagcgccag cgcgctctcc
     6121 cggtccgggc aggacacccg cacgatatcc accccggcca gctcggcccg ccggatctgg
     6181 gcgaccgtgc cctccacgtc ggtggtcagc gtgttggtca tggtctgcac gctgatcggc
     6241 gcgccgtcgc ccaccggcac gttgccgaca tacacccggc gcgacttgcg gcggatgatc
     6301 tcctggtagg ggcggtagct catcaggcag gctcctccgt catgtccatg ccggcgaggc
     6361 cggctttggc ttgcaagtgg gggctccggc gcgatcccgc taccgggatg gggtgcgaag
     6421 gctggcctgc tggacgccgt tgccggaggg cgggttcagc gccccctgct tgatcagggc
     6481 cggatccagc ggcaggtcgc gccgcaccgc ccccggggcg ccaagcaggc cggtcgagac
     6541 gccgttcaac tccagttcgg tgccgccggc attgccggtg gtcagcagca ggttgggacg
     6601 atccggcacc tgccagctgt cgccggcatg caggatgcgg ttcagcagta cctggccgga
     6661 cttgtcgcgc acctgcaccc aggaatccgc cgtcgcgcgc agcacgacct gtggttgctg
     6721 tggggtggat gccgcggcgc tgtcagcggc agccgggacc tgcggctggg cggcaaccag
     6781 ctggccggcc agcacggtct gcggcggagg ctgcgcggcg gtggcgccgg ggttctgcgg
     6841 cgaggcgccc gcctgggcgg cgtcaggctg cttcagcggc ggcgtcaccg aagccgcagc
     6901 cgccgaggtg ggagagacca cctgggcagg cggctcggcg gcctcggcgc tggtcggcga
     6961 ggggtcctgc gccggggttg ccgcggcaac gctggtccgg gggagcttgg ccacgggcgg
     7021 catcgcctgc tgcgccagag tctgcagatg ctgcggaatg gcaatggatt ccggcggcag
     7081 gcggccttca ccggacaagc ggtaccagcc ggcataggcg ccgatcgcca gcaccaggcc
     7141 cagcagcacg acggcaccgg cgggaatgcc gcgctcgggc atgggggcgg ggaaggccag
     7201 ctcggtgcgt gtgttcaccc tgccagcttc cgccttgaat cggcggacaa tctcctcggg
     7261 gtccaggccc agcgaggcgg cgtaggtgcg caggaatccc agggcatagg cgtgcgccgg
     7321 cagcaggtcg atgcggcctt cctccagcgc ctccagatgc ggctggcgga tgcgcagccc
     7381 ggcggcaatc tcggacagat cccagcccaa ccgttctcgc gcggcacgca ggtccgcccc
     7441 ggcacgggcg gcgagcggcg gttcgtcctg ctccggaccc accggagaca tcatcgttcg
     7501 actttcggcc agcttcgacc tgcgatcctg cagcacggtc gccccgccct tctatagctt
     7561 cagttcaccg gtgagaacac gccagccgaa ccctgccgca gcgcaacctg cccgcccggt
     7621 gacacacgcg ttctaaaccg aggccggccc tttccgccag ccgctttcac attactccgt
     7681 tttgtgacgg cgtcgatgct gtattgcgga aggaattgaa aaccatgcgg attgtcacca
     7741 gggggtcaca agcgcgcggc gcgccgggag caattttccg tccagccatg caagccgcgc
     7801 gggttcaggc cggcaatccg tgctctcgcg cccaggttgc aatcggttcc cgcaagctcg
     7861 cagtgccggc gccaccccgc cggatcgacg ccagcaccgg ccggaatgcc gtcaggtcga
     7921 gctgggccag cagggccttg accggcagga tgccggcggc cggcatcgac aggttggtca
     7981 gccccaaccc gaccagcgcc agcgcttcca gcggccgcga cgccgcctcg ccgcagatcg
     8041 acaacggcac cccggcgccg ttccgcgcgg cccgcgccgc ggtcagcaac tgatccagca
     8101 ggtcgaacac cggctgcgac agcaggtcgt accgggcata cagcgacggc gtgccccggt
     8161 cggcggcgaa caggaactgc agcaggtcgt tggagccgac cgagacgaaa tccacctcct
     8221 tgagcagctc cggcagctgc cacatcagcg ccggcacctc cagcatcgtg ccgatcgaca
     8281 gccggtccgg cgcgggacgc acccggcggg cctcggcctg cagcaaggcg cgggcggcgc
     8341 ggaattccgt cacggtcgcc accatgggga acatcaccgt cagcggacgg ccgccggcgg
     8401 ccagcagcag ggcgcgaagc tgccggcgca gcagggcagg gcggtccagc ccgatgcgca
     8461 gcgaccgcca gcccatcgcc gggttctcct cctcggaaat cgtgcccggc agcatcttgt
     8521 cggcaccgag gtccagcgtg cggaacacca ccggccggtc gccggcggca tccagcacgc
     8581 gggcgtagag cgccgcctgt tccgccacgt cagccaccga gccgcgtgcc atcatggcga
     8641 tctcggtgcg gaacaggccg atgccgtcgg ctccggtgct gtcgagctgc gccagctcca
     8701 gtgccaggcc ggcattcagc atcagctcca cgcgggtgcc gtcggcggtg cagccgggct
     8761 tgtcgcgcag cgtggcgtag cctgcctgga gcgtcgtctg gttttccagg gcgcgggcat
     8821 acacctcccg cacatccgcc tccggccgga gcacgagctg gccttcgtcg gcatcgatga
     8881 tcgcctcgtc gccctgttcc gcggtgtcca gcatgccgcg cacgccgccc accgccggga
     8941 ttcccagggc gcgggccagg atggcggcgt ggccggcagg gctgccctcc tcgatggcga
     9001 tgccggcaat gccctgggcg tgccagtcca gcagttccgc cgggccgagc cggcgggcga
     9061 gcaggatgct gccgggcgga acctcggggc ggggaacgtc accgatcagg gcggaaagca
     9121 aacgtcccgc gaggtcctcg atatcggcca gccgctcgcg cagatagggg tcggtcacgc
     9181 gccgcatgcg atcatgcagg tcgccggcca cgcgctgcac cgcggcctcg gcggacacac
     9241 cggtgcggat cacctcctcc acacggtgca tccatccggc atccgaggcg accagccggt
     9301 aggcatccag cacttcccgc gaggcgtgcg catcggcatc gccgcccccg tccagggcgt
     9361 tgcccttgat gagctcgtcc agcccgcgct gcatgcgctc gctggcgccg tgcaggcggg
     9421 tcagttccgc catcgggtcc tccgccagca ggggcacctg cacgcgcggc atgccatgca
     9481 gcacgaccgg accgatggcg atgcccgcgg tcagcgacga tccggcgaac acccggggca
     9541 ccgtggcggc aagcccctcg ggggcgccgt cggtcgcgcc actggcgggc agcagctctg
     9601 ccaccagcat ggcgacggtc tccatctcat cgacttcctc ctcggtgtag tgccgaggat
     9661 tgcggttctg ggcggcgatc acgcccaaag tgcggccggc tcggcgcacc ggtacggcaa
     9721 gcatcgaggc gtagggttcc tcgccggtct ccggccggta ggcgaaggcg ggatggttct
     9781 gggcatccgg caggttcatc accgcgccgg tggcggcgca caggccgaca atgccctcgc
     9841 ccacgcgcag gcgggtatgg ccgatcgcgt cagggttcag accgacggtg gcgaccagtt
     9901 ccaggatgtc gcccggcttc tgggcgtaga tcgagcagac ctcggcgacc agctccgagg
     9961 cgaccagccg caccagctca cccagcggcg ccgacccgct ggcccggagg tcgcgaagcc
    10021 gaaccagcag gctacgggtg ccgggcatgc gcgggctacc aggccggggt gggggtgggg
    10081 gcggcgttca
//
LOCUS       DASSZH010000328         2535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_587220, whole genome shotgun sequence.
ACCESSION   DASSZH010000328 DASSZH010000000
VERSION     DASSZH010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587220"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VFN42_07075"
     CDS             complement(<1..506)
                     /locus_tag="VFN42_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003623877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8996414.1"
                     /translation="MSAERPILIVDDDLALRETLAEQLAVDGEFTAAEAGSVAEAEER
                     LLSKDARFDAVILDVGLPDGDGRDFCVKLRRQGQKMPIIMLTGSDAETDVVRGLDSGA
                     NDYIAKPFRLNELLARLRAQLRIFENSEDAVFSIGPYTFRPSAKLLQDPAKNRRIRLT
                     EKEAAILK"
     gene            971..1996
                     /gene="ribA"
                     /locus_tag="VFN42_07080"
     CDS             971..1996
                     /gene="ribA"
                     /locus_tag="VFN42_07080"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HET8996415.1"
                     /translation="MPVLLAGPTPLVVIAAETAGPDGLADLAAWAADTPVLLLALTRA
                     AAVLRRPVEQVAAAVALRLPAAARAPEVLRALADPMLDRAVLDPLDTAPLPQGAEAAL
                     ALAKLARLLPAVLAAPAATDPGERPALLDVEAAEILAYPAAAAAGLRQVAAAQVPLES
                     APDARVVAFRTESAGLEHLAILVGAPEQQAAPLVRLHSECFTGDLLGSLRCDCGQQLR
                     GALTRMAEEGGGVLLYLAQEGRGIGLVNKLRAYALQDRGLDTLDANRALGWGADERNF
                     LVAATMLEQLGLKRIRLLTNNPDKLTALAACGVEIVGREAHSFAPNGVNDEYLATKAA
                     RFGHLLD"
     gene            2035..2496
                     /locus_tag="VFN42_07085"
     CDS             2035..2496
                     /locus_tag="VFN42_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HET8996416.1"
                     /translation="MLRGTVFRVALGRGGVRQDKQEGDGATPAGLLPLRRVLYRADRL
                     PPPDCTVPVEPIAETDGWCDDPGARDYNRQVRLPHDARHEELWRRDGLYDVVGVLGWN
                     DAPVVRGRGSAIFLHVARPDYGPTEGCVALALPDLRRVLAMGVTALQVLAS"
BASE COUNT          355 a          880 c          879 g          421 t
ORIGIN      
        1 aacttcagga tcgccgcttc cttctcggtc agccggattc ggcggttctt cgcggggtcc
       61 tgcagcagct tcgccgaggg gcggaaggtg tagggtccga tgctgaacac ggcatcttcg
      121 ctgttctcga aaatccgcag ctgtgcccgc aagcgggcca gcagctcgtt caggcggaac
      181 ggcttggcaa tgtaatcgtt ggcgcccgaa tcgaggccac gcaccacgtc ggtttccgca
      241 tccgagccgg tcagcatgat aatgggcatt ttctgcccct gccggcgcag cttgacgcag
      301 aaatcccggc cgtcgccgtc cggcagcccc acgtccagga tgaccgcgtc gaaccgcgca
      361 tccttggaca gcaggcgctc ctccgcctct gcgaccgagc cggcctctgc cgccgtgaac
      421 tcgccgtcca ccgcaagctg ttctgccagg gtctcgcgga gggcgaggtc gtcatccacg
      481 atcagaattg gtcgttcagc cgacatctct ggttcccttt gccaatgacg cggcacggga
      541 aggctcccgc gcagcgacag gttccgacgc cgcgccagcc tagaggacta gcgcgcgctt
      601 gcgtaatgga tgcctcacga accgggtttg taccacatca cattccggcg tggtgctgga
      661 tgtctcacaa attcgtgtcc gtgaccgcga catccacccc gctccgccat atactgcagt
      721 ccgaagtggt tccgtggagc cggacggctc ctcgcagcat ctgaaagcaa ggcttcccgt
      781 gaatcttgac caaattgggt ccgcatcgtc gtctctccat gtagcgtgaa cggccccggt
      841 tccaatgacc ctctctcctt ccgccgcccg actgtccgga aagcaaaccg gttccccgca
      901 agccggggaa gccggtacaa cccgccgttt gcgccgtatt catcgcgctg cctcggaatt
      961 gcggcgcggc gtgcccgtgt tgctggccgg ccccacgccg ctggttgtga tcgcggcgga
     1021 gaccgccggc cccgacggat tggccgatct cgccgcctgg gcggccgaca cgccggtgct
     1081 gttgctggcc ctgacgaggg ccgccgcggt gctgcgccgc ccggtggagc aggtcgccgc
     1141 cgccgtggcc ttgcgcctgc cggccgcggc gcgcgcaccg gaggtgctgc gtgccttggc
     1201 cgatccgatg ctggaccgcg cggtgctgga tccgctcgac acagcaccgc tgccgcaggg
     1261 cgccgaggcg gctctggcgc tggccaagct ggcacgcttg ttgcccgccg tgctggcggc
     1321 tcccgccgcg acggatccgg gcgagcggcc cgcactgctc gatgtcgagg cggcggagat
     1381 ccttgcctat ccggccgccg ccgccgccgg cctgcgccaa gtcgccgccg cacaggttcc
     1441 gctggaatca gcgccggatg cccgcgtggt ggcattccgc accgaaagcg ccggcctgga
     1501 gcatctggcg attctggtcg gcgcgccgga gcagcaagcc gcgccgctgg tccgcctgca
     1561 ttccgagtgt ttcaccggcg acctgctggg cagcctgcgc tgcgactgcg gccagcagct
     1621 gcgcggtgcg ctgacccgca tggcggaaga aggcggcggc gtgctgctgt acctggcgca
     1681 ggagggcagg ggcatcggcc tggtgaacaa gctgcgcgcc tatgccctgc aggatcgcgg
     1741 cctggacacg ctggacgcca accgcgcgct gggctggggc gccgacgagc ggaatttcct
     1801 ggtcgccgcc accatgctgg agcagcttgg cctcaagcgc atccgcctgc tgaccaacaa
     1861 tccggacaag ctgaccgcac tggcagcctg cggcgtggag atcgtcgggc gggaggcgca
     1921 cagtttcgcg ccgaacgggg tcaacgacga atacctcgcc accaaggcgg cgcgcttcgg
     1981 ccatctgctg gactgatccc ggtgcaggcc caggttcatc ctgacggccg cctgatgctg
     2041 cgcggcacgg ttttccgcgt cgcgctgggg cgcggtgggg tgcggcagga caagcaggaa
     2101 ggcgacggcg cgacccccgc ggggctgctg ccgctgcgcc gcgtgctgta ccgcgccgat
     2161 cgcctgccgc cgccagattg cacggtgccg gtcgagccga tcgccgaaac ggatggctgg
     2221 tgcgacgatc ccggtgcgcg cgactacaac cgccaagtcc ggttgccgca cgacgctcgc
     2281 catgaggagc tctggcggcg cgacgggctg tatgacgtgg tgggcgtgct gggttggaat
     2341 gatgcgccgg tggtccgcgg gcgcggttcg gcgatcttcc tgcacgttgc ccgcccggat
     2401 tacgggccga cggaaggctg cgtcgcgctg gcgctgccgg acctgcgccg cgtgctggcg
     2461 atgggcgtca ccgcgctgca ggtgctggcg tcgtgaagga ctcctccccc tccccttgcg
     2521 ggagggggga ggggg
//
LOCUS       DASSZH010000329         4892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_859997, whole genome shotgun sequence.
ACCESSION   DASSZH010000329 DASSZH010000000
VERSION     DASSZH010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859997"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VFN42_07090"
     CDS             <1..336
                     /locus_tag="VFN42_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996417.1"
                     /translation="EPHEYARFPEFFKVPDGRKRITWSYIDARDLGQIVRLAVEKDGL
                     GYQVFNAAQDDCSSDLPTAELLRRFYPNVKLTRPIGEYETLLANRKAREMLGFRPAHS
                     WRQELAAPR"
     gene            complement(290..535)
                     /locus_tag="VFN42_07095"
     CDS             complement(290..535)
                     /locus_tag="VFN42_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996418.1"
                     /translation="MMPTLRSVAAEEIAMCQDVRRALTFETLLTDPLVRLMMAADGVT
                     PQDLVTPLLVAREAIAARELAALSAALRAPDARNGRA"
     gene            complement(597..1916)
                     /locus_tag="VFN42_07100"
     CDS             complement(597..1916)
                     /locus_tag="VFN42_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996419.1"
                     /translation="MSQPDPFLPAPTAEEPPPPAAPAAAAPGGHPLARKTELEAAMAA
                     APRDAGLRAAYFEHLGRLASTQTGLFWALLPEIGAPLWFRGGTPDIAALAAAFRDNAY
                     AVPDLRATPQRILVIGAHAGYSALALARLHPRAVLLCAEPLADNLRLLALNTTPCRRI
                     RVAQTALWHSATRLAPTGRLQADWMVRLTDEALDADRVIPAMSVPELLARAGWTHADM
                     VFCDASGSEREIFIDPLAPWLQRLDVAVVRTYDPLATQASELVAACFAGDSFSHRTHG
                     ALQVHERRTPLTALPRLPAEVFLLRAEPGGAPFGLQDVASQGWAFFVFDGSSCQLHPN
                     GPGEAPARAIFPVRLDGHARFVSLVRHAGNPTSPAVVFTARLARMDGTVVAQGTATVS
                     GHGSDRIAFDLPAGLQGPMLAVLETAMAADAPSNAMAWARWIDPRLS"
     gene            2093..2497
                     /locus_tag="VFN42_07105"
     CDS             2093..2497
                     /locus_tag="VFN42_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET8996420.1"
                     /translation="MSPLQHARLPSILRDTVVSLVRREGPDLTARQLGVLLICYLEEG
                     PHTVRGLAARLNVAKPAITRALDRLEEFQFARRLQDPRDRRSIIVARTEEGEQFMAQL
                     QGLLDEASRNTSPPRTSALRHIPAYEPLAAVG"
     gene            complement(2588..>4892)
                     /locus_tag="VFN42_07110"
     CDS             complement(2588..>4892)
                     /locus_tag="VFN42_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019084967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HET8996421.1"
                     /translation="ANYLSVGQVYLLDNPLLRETLAPAHIKPRLLGHFGTVPGLNFIY
                     VHLNRLIRHHDLDMIYICGPGHGAPGVIANTWLEGTYSETYPAVSHDADGMRALFRQF
                     SFPGGIPSHAAPETPGSIHEGGELGYSLSHAFGAAFDNPDLIVACVVGDGEAETGPLA
                     TSWHGNKFLNPRTDGTVLPILHLNGYKIANPTILGRISREELVKLFEGYGYTPHIVEG
                     DDPAVLHAQMAATMDRILGEIRAIRAGAGEGEHPRWPMLILRTPKGWTGPKEVDGKRV
                     EGFWRAHQVPIADMSTDAHVALLESWMRSYRPEELFDENGRPRPGILDLAPRGNRRMS
                     ANPHANGGILRRDLILPDCTQYAVEVGHPGEHDRESTAVMGAYLRDVFRHNAAMRNFR
                     LFGPDETASNRLQATFEATDRAWDAETLPYDENLAPTGRVMEILSEHTCQGWLEGYLL
                     TGRHGLFNCYEAFIHIVDSMLNQHAKWLKSSKALAWRRPISSLNYLLTSHVWRQDHNG
                     FSHQDPGFIDYVVNKKADTVRVYLPPDANTLLCVTEHCLRSYDRINVIVAGKQPQPQW
                     LSMDAAVKHCAAGIGIWEWASNDEGTEPDVVMACAGDVPTLETLAAVSLLHEHLPDLR
                     VRVVNVVDLMTLVPAYAHPHGLSDHDFDALFTRDKHVVFAFHGYPTLIHRLTYRRSNH
                     ANFHVRGYQEEGTTTTPFDMVVLNGLDRFHLVGDVIDRLPELGPRAAYAKQTIRDKLI
                     DHRRYIAAHGLDMPEVREWRWPGLPPM"
BASE COUNT          750 a         1696 c         1679 g          767 t
ORIGIN      
        1 gaaccgcatg aatacgcgcg gttcccggag ttcttcaagg tgccggacgg ccgcaagcgg
       61 atcacctgga gctatatcga tgcgcgcgac ctggggcaga tcgtgcgcct cgcggtcgaa
      121 aaggacggtc tgggctatca ggttttcaac gccgcccagg acgattgctc ctcggacctg
      181 ccgacggcgg aactgctgcg gcgcttctac ccgaacgtga agctcacgcg cccgatcggc
      241 gaatacgaaa cgctgctcgc caaccggaag gcgcgcgaga tgctcggctt caggcccgcc
      301 cattcctggc gtcaggagct cgcagcgccg cgctgagggc ggcgagttcg cgcgcggcga
      361 tggcctcgcg cgcgacgagc agcggggtga cgaggtcctg cggcgtcacc ccgtcggccg
      421 ccatcatcag gcgcaccaac gggtccgtca gcagcgtctc gaacgtcaga gcgcgccgaa
      481 catcctgaca catcgcaatt tcctccgccg caacagatcg aagagtgggc atcatgccgt
      541 ctccacgctg cttccgcgga gttaattgcg cgtcagatac tttgcgttac atcccgtcaa
      601 ctcagccgcg gatcgatcca gcgcgcccag gccatcgcat tgctcggcgc gtcggcggcc
      661 atcgccgtct ccagcaccgc gagcatcggc ccctgcagcc cggccggcag gtcgaaggcg
      721 atgcggtcgc tgccatgccc ggacaccgtc gccgttccct gcgccaccac ggtcccgtcc
      781 atgcgcgcga gccgcgccgt gaacaccacg gcgggcgagg tcgggttgcc ggcgtgccgc
      841 accaagctga cgaagcgcgc atgaccgtcg agccgcaccg ggaagatcgc ccgcgccggc
      901 gcctcgcccg ggccgttcgg atgcagctgg cagctggagc cgtcgaacac gaagaaggcc
      961 cagccctggc ttgccacgtc ctgcagcccg aacggcgcgc cacccggctc ggcgcgcagc
     1021 aggaacacct ccgccggcag gcgcggcagc gcggtcagcg gcgtgcgccg ctcatggacc
     1081 tgcaacgcgc catgcgtgcg gtgggaaaag ctatccccgg cgaaacaggc cgcaaccagt
     1141 tccgaggcct gcgtcgccag cgggtcgtag gtccgcacca ccgccacgtc gagccgctgc
     1201 agccagggcg ccagcgggtc gatgaagatt tcccgctcgc tgccggaggc atcgcagaac
     1261 accatgtcgg catgcgtcca gccggcccgc gccagcagct ccggcaccga catggcgggg
     1321 atgacgcggt cggcgtcgag tgcctcgtcg gtcagccgca ccatccagtc cgcctgcagc
     1381 cgcccggtcg gcgcaagccg ggtcgcggaa tgccacagcg ccgtctgcgc gacccggatg
     1441 cgccggcagg gcgtggtgtt gagcgccagc aggcgcaggt tgtcggccag cggctcggcg
     1501 cagagcagca ccgcccgtgg gtgcagccgc gccagcgcca gggccgaata gccggcatgg
     1561 gcgccgatca ccaggatgcg ctgcggggtc gcgcgcaggt cgggcacggc ataggcgttg
     1621 tcgcggaagg cggcggcgag ggccgcgatg tccggcgtgc cgccgcggaa ccacagcggc
     1681 gcgccgatct ccggcagcag cgcccagaac agtccggtct gcgtgctggc cagccgtccc
     1741 agatgctcga aataggcggc gcgcagcccc gcgtcgcgcg gcgcggccgc catcgccgcc
     1801 tccagctccg tcttgcgggc aagcggatgc ccgcccggcg cggcggcggc cggagcggcc
     1861 ggcggcggcg gttcctcggc ggtcggagca ggcaggaacg ggtcgggctg agacatcggg
     1921 cacgggtcct ttccgcccca ccttccgcga tttcacgcgg cttggcgagg ccgccgcgca
     1981 cccccgaaat cccattttcc gcaattaatg cacctagatc attttttcga gatgtgccat
     2041 tcttgtcatg ttgtcatatc ccggcgcgcc gccgccggtg ggggtccctg aaatgtcacc
     2101 actgcagcac gcacgcctgc ccagcatcct gcgcgacacc gtcgtcagcc tcgtccgccg
     2161 cgaggggccg gacctgacgg cccgccagct cggggtgctg ctgatctgct accttgagga
     2221 aggtccgcac accgtgcgcg ggctggccgc gcggctgaat gtggccaagc cggcgatcac
     2281 gcgcgcgctg gaccggctgg aggaattcca gttcgcgcgc cgcctgcagg acccgcgcga
     2341 ccgccgcagc atcatcgtcg cgcgcaccga ggagggcgag cagttcatgg cgcagctgca
     2401 ggggctgctg gatgaggcgt cgcgcaacac ctccccgccc cgtacctcgg cgctgcgcca
     2461 catcccggca tacgaaccgc tcgccgccgt cggctgaccg gcagcccgcc ccccactcca
     2521 tcccgtggcg aagggcgggc ttcgttcccg ccctctccta catcggcgtc tcctacatcg
     2581 gcgtctccta catcggcggc aggcccggcc agcgccactc ccgcacctcc ggcatatcca
     2641 ggccgtgcgc cgcgatgtag cggcggtggt cgatcagctt gtcgcggatc gtctgcttgg
     2701 cataggcggc gcgcgggccg agttccggca ggcggtcgat cacgtcgccg accagatgga
     2761 accggtccag cccgttcagc accaccatgt cgaagggggt cgtcgtcgtc ccctcctcct
     2821 ggtagccgcg cacgtggaaa ttggcgtggt tgctgcggcg gtaggtcagg cgatggatca
     2881 gcgtcggata gccgtggaag gcgaacacga cgtgcttgtc gcgcgtgaac agcgcgtcga
     2941 agtcgtggtc cgacaatccg tgcggatgcg cgtaggcggg caccagcgtc atcagatcga
     3001 ccacgttcac cacgcgcacg cgcaggtcgg gcagatgctc gtgcagcagc gaaaccgccg
     3061 ccagcgtctc cagcgtcggc acgtcgccgg cgcaggccat gacgacgtcc ggctcggtgc
     3121 cttcgtcgtt gctcgcccac tcccagatgc cgatgccggc ggcgcagtgc ttcaccgccg
     3181 cgtccatgct cagccattgc ggctgcggct gcttgccggc gacgatcacg ttgatgcggt
     3241 catagctgcg caggcagtgt tccgtgacgc agagcagcgt gttggcatcc ggcggcagat
     3301 agacgcgcac cgtgtcggcc ttcttgttca ccacgtagtc gatgaagccg ggatcctggt
     3361 ggctgaaccc gttgtggtcc tgccgccaga catgcgaggt gagcaggtag ttcagcgagg
     3421 agatcggccg ccgccacgcc agcgccttgc tgctcttcag ccatttcgca tgctggttca
     3481 gcatggaatc gacgatgtgg atgaacgcct cgtagcagtt gaacagcccg tgccgcccgg
     3541 tgagcagata gccctccagc cagccctggc aggtatgctc gctgaggatc tccatcaccc
     3601 ggccggtggg cgcgaggttc tcgtcatacg gcagggtttc cgcgtcccag gcgcgatccg
     3661 tcgcctcgaa cgtcgcctgc aggcggttcg acgcggtttc gtccgggccg aacaggcgga
     3721 aattgcgcat cgccgcgttg tgccggaaca cgtcgcgcag ataggcgccc atcaccgcgg
     3781 tcgattcgcg gtcatgctcg ccgggatggc cgacctcgac cgcgtattgc gtgcagtcgg
     3841 gcaggatcag gtcgcggcgc aggatgccgc cgttggcgtg cggattggcg ctcatccgcc
     3901 gattgccgcg cggcgccagg tccagaatcc cgggacgtgg acggccgttt tcgtcgaaca
     3961 gttcctccgg ccggtagctg cgcatccatg attcgagcag cgcgacatgc gcatccgtgc
     4021 tcatgtcggc gatcggcacc tgatgcgcgc gccagaagcc ttccacgcgc ttgccgtcca
     4081 cctccttcgg tccggtccag cccttcggcg tgcgcaggat cagcatcggc cagcgcggat
     4141 gctcgccctc gccggcgcct gcgcggatcg cgcggatttc gcccaggatg cggtccatcg
     4201 tcgccgccat ctgcgcatgc agcacggcgg ggtcgtcgcc ctcgacgata tgcggggtgt
     4261 agccgtagcc ctcgaacagc ttgaccagtt cctcgcggct gatccggccg aggatggtcg
     4321 ggttggcgat cttgtagccg ttcaggtgca ggatcggcag caccgtgccg tcggtgcgcg
     4381 gattgaggaa cttgttgccg tgccaggagg tggcgagcgg cccggtctcc gcctcgccgt
     4441 caccgaccac gcaggcgacg atcaggtcgg ggttgtcgaa ggcggcgccg aaggcgtggc
     4501 tgagcgaata gcccagttcg ccgccttcgt ggatggagcc gggggtttcc ggggcggcgt
     4561 ggctcgggat gccgccgggg aaggagaact ggcggaacag cgcgcgcatg ccgtcggcat
     4621 cgtggctgac ggcgggatag gtttccgaat acgtcccctc cagccaggta ttcgcgatca
     4681 cgccgggtgc gccgtggccg gggccgcaga tatagatcat gtccaggtcg tgatgccgga
     4741 tcagccggtt caggtggaca tagatgaagt tcaaccccgg caccgtgccg aaatgcccga
     4801 gcagccgcgg cttgatgtgc gcgggcgcca gggtttcgcg cagcagcgga ttgtccagca
     4861 gatagacctg accgaccgaa agatagttgg cg
//
LOCUS       DASSZH010000330         4880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_740730, whole genome shotgun sequence.
ACCESSION   DASSZH010000330 DASSZH010000000
VERSION     DASSZH010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4880
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740730"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VFN42_07115"
     CDS             <1..487
                     /locus_tag="VFN42_07115"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HET8996422.1"
                     /translation="SAMLAAPALAATTTTGPAGSPGTAAPDTATHAPATPPGGTMGTS
                     HTAAGPASAGQPATAANKPAPPKFTTADNQLRAGKLVGASVYNDKNQSVGSINDILLS
                     QDQDKAQTAVISVGGFLGMGSKLVSVPFEKLKIEKDKVIMPGATEQALKQMPAYKFNR
                     A"
     gene            705..1907
                     /gene="egtB"
                     /locus_tag="VFN42_07120"
     CDS             705..1907
                     /gene="egtB"
                     /locus_tag="VFN42_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HET8996423.1"
                     /translation="MDEAALRLQVTSGEAVSARYQAVRRHTDSLVAALTPEDQCVQSM
                     PDASPAKWHRAHTTWFFEQFVLSPFLPGYQAFDPKFRYLFNSYYEAVGPRHPRPSRGM
                     LTRPGVAEVAAYRSHVDAAMADLLPRLPREAAALVELGLQHEQQHQELLLTDMLHAFA
                     QNPLAPAILPGWRDPAAASGEPGWTTCSGGIVHIGHAGRDFCFDNETPAHQTLLHAHA
                     IGTHLVRNRDWLAFMQDGGYRIPTLWMSDGWATVQSQAWSAPLYWQEQEGAWWQMNLA
                     GLAPIDPNAPVRHVSWYEADAFARWAGARLPTEAEWEAACRRGDLREADSHVWQWTSS
                     AYSPYPGYRPVAGAVGEYNGKFMINQMVLRGGSLATPPGHSRPSYRNFFHPDKRWQFS
                     GLRLARDA"
     gene            1971..2897
                     /gene="egtD"
                     /locus_tag="VFN42_07125"
     CDS             1971..2897
                     /gene="egtD"
                     /locus_tag="VFN42_07125"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HET8996424.1"
                     /translation="MADLALAGLRRPQKSLPPQLFYDEEGCRLFYRITELPEYYLTRT
                     ERALLADIAPDVLRRVPSAGVLVEYGASDEAKALHLLQSAGQTSLRAYVPVDVAAAAL
                     EAMRTRLGRQLPWLSVHPVARDFMQPITLPPAVAGLARLGFFPGSTIGNLDPEASVAF
                     LRSARRTLGPRAQFLVGADLRKHPDVLLPAYDDKAGITAAFNRNLLVRLNREAAADFD
                     LNRFAHRTVWNDAASRIEMHLVSSCDQVVLVAGQPIHFTAGETIHTENSYKHTPEAFL
                     EITGAAGWGCREIWTDPKQWFALYLLEPRAAA"
     gene            complement(2901..3704)
                     /locus_tag="VFN42_07130"
     CDS             complement(2901..3704)
                     /locus_tag="VFN42_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HET8996425.1"
                     /translation="MTRTVAIAGLGAIGLPLARALDAGVEGLTLVAVAVRDQGKAAAN
                     LADFQRPPKLVEIPALAEADIVVEAAPAAVFEPIARAAIEAGRIFIPASVGALLSRMH
                     LVHQAQATGARVIVPTGALLGLDAVRAAAEGPMESVTMVTRKPPAGLAGAPYLERHGI
                     QVAGLTEPLCVFRGNALDAAAGFPANVNVAAALALAGVGPRRTQVEIWADPGVTRNIH
                     TIRVDAAAACFTMTIENVPSEENPRTGKLTPLSLLACLRGLVSTLKVGS"
     gene            complement(3701..4312)
                     /gene="mobA"
                     /locus_tag="VFN42_07135"
     CDS             complement(3701..4312)
                     /gene="mobA"
                     /locus_tag="VFN42_07135"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR02665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HET8996426.1"
                     /translation="MMPRERIAALILAGGTARRMGGGDKPLLDIGGRTMLQRVIAALG
                     SEAAALAISANGDPARFAGFGLPVLPDGCFAGEGPLAGLLAGLDWAADQGVFALLSVP
                     GDTPFVPAGLAASLDPPPACVVSGGRAHHLVALWPVACRDRLRTLLSAPGPRSVARFA
                     GEIGARRVDFAAGTWDPFFNINTPDDLAQARALAAKAAAGEQS"
     gene            complement(4323..4398)
                     /locus_tag="VFN42_07140"
     tRNA            complement(4323..4398)
                     /locus_tag="VFN42_07140"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4363..4365),aa:His,seq:gtg)
     gene            complement(4492..>4880)
                     /locus_tag="VFN42_07145"
     CDS             complement(4492..>4880)
                     /locus_tag="VFN42_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET8996427.1"
                     /translation="LGVISGKGLECVITLGTGMGFALFDDGRPAPHLELSQHPSHRGK
                     TYDEWIGNAALKSAGAKRWNKRVRKAIGWIETLVNYDVLLIGGGNAKRLTGELPPNVR
                     TVPNQAGITGGIRLWDPALDELVANA"
BASE COUNT          863 a         1736 c         1531 g          750 t
ORIGIN      
        1 ctcggcaatg ttggccgccc cggcactggc cgccacgacc accacaggtc ccgccggcag
       61 ccccggcaca gccgcccccg ataccgcgac ccatgcaccg gcaacgccgc ccggcggcac
      121 catgggcacc agccacaccg ccgccggtcc ggccagcgcc ggccagccgg cgactgcggc
      181 gaacaagccc gcgccgccca agttcaccac cgccgacaac cagctgcgcg ccggcaagct
      241 ggtcggcgcc agcgtgtaca acgacaagaa ccagtcggtc ggctcgatca acgacatcct
      301 gctgtcgcag gaccaggaca aggcgcagac cgcggtgatt tcggtcggcg gcttcctcgg
      361 catgggcagc aagctggtct cggtgccctt cgagaagctg aagatcgaga aggacaaggt
      421 gatcatgccg ggtgccaccg agcaggcgct gaagcagatg cctgcctaca agttcaaccg
      481 cgcctgaatc gatcgcgtcc ggcccgggac ggacaggctg atgacggcat tggcccgctg
      541 acggttcgac cacgcagcct ggtcacgtgc cgtcgcaggc aggggtggat gggcaaccat
      601 ccaccccagg ccggcgtcat gcattggcgt cgtctgcgcg gcctgccata gtgcggcgac
      661 cgacgccgcc cgcctttgcc gttctgccag accggagccc gagcatggac gaggctgccc
      721 ttcgcttgca ggtcacgagc ggcgaggccg tatcggcgcg ctaccaagcg gtgcgccgcc
      781 acactgactc tctggtggcc gccctgacgc ccgaggatca gtgcgtccag tcgatgccgg
      841 acgccagccc ggccaagtgg catcgcgccc ataccacgtg gttcttcgag caattcgtgc
      901 tctccccctt tctgcccggc tatcaggcct tcgacccaaa gttccgctat ctgttcaact
      961 cctactatga ggccgtcgga ccacgacacc cgcgcccatc ccgcggcatg ttgacccgcc
     1021 caggtgtggc ggaggtggcc gcctatcgct cccacgtcga cgcggcgatg gcggatctgc
     1081 tgccgaggct gccgagggaa gcggcagcgc tggtggagct cggccttcag cacgaacagc
     1141 agcaccagga actgctgttg acggatatgc tgcacgcctt cgcacagaat ccgctggccc
     1201 ccgcaatctt gccgggatgg cgtgatcccg ccgccgcctc cggggagcca ggctggacaa
     1261 cctgctccgg cgggatcgtc catatcggcc atgccgggcg agacttctgc ttcgacaacg
     1321 agaccccggc gcatcagacg ttgctgcatg cgcatgccat cggcacccac ctcgtgcgca
     1381 atcgcgactg gctcgccttc atgcaggacg gcggttatcg catcccgaca ttatggatgt
     1441 ccgatggatg ggcaacggtc cagtcgcaag cctggtcggc tccgctctac tggcaggaac
     1501 aggagggcgc ctggtggcag atgaacctcg ccggcctggc gccgatcgat ccgaatgcgc
     1561 cggtgcggca cgtcagctgg tatgaggcgg atgctttcgc ccgctgggcc ggcgcccgcc
     1621 tgccaaccga agcggaatgg gaggcagcct gcaggcgcgg cgacctgcgg gaggcggact
     1681 cccatgtctg gcaatggacc tccagcgctt acagcccata tccgggatac cgcccggtcg
     1741 ccggcgcggt tggggagtac aacggcaagt tcatgatcaa ccagatggtg ctgcgcggtg
     1801 ggtcgctggc gacaccgccc ggccattccc gtccgagtta tcgaaacttc ttccatccgg
     1861 acaagcgctg gcagttttcc ggactgcgcc tggcacgcga cgcctgaacc gaaagccccg
     1921 caccaatgcc gaacgactcc ggcctacgcg ccgccgtcac agatcgcagc gtggcggacc
     1981 tggccctggc gggcctgcgc cgaccgcaaa aatcactgcc gccacaactg ttctatgatg
     2041 aggaaggctg ccggctgttc tatcgcatca cggaactgcc ggagtactac ctcacccgta
     2101 ccgaacgcgc actgctcgcc gacatagccc cggacgtgct gcggcgggtt ccgagcgcgg
     2161 gggtgctggt ggaatacggg gccagcgatg aggccaaggc actgcacttg ctgcaatcgg
     2221 cggggcagac ctcgttgcgg gcctatgtcc cggtcgatgt cgccgccgcc gcgctggagg
     2281 cgatgcgtac ccgcctgggg cggcagcttc catggctgtc ggtgcacccg gtggcgcgcg
     2341 acttcatgca accgatcacg ctaccccccg cggtcgcggg actggcacgt ctgggcttct
     2401 ttcccggatc gacgatcggc aacctggacc cagaagcctc cgtcgctttc ctgcgctccg
     2461 cccgtcgcac gctcggcccg cgtgcgcagt tcctggtggg ggcggatctc cgcaagcacc
     2521 cggacgtgct gctgcccgcc tatgatgaca aggcgggcat caccgccgcg ttcaatcgca
     2581 acctgctggt gcgattgaac cgcgaagccg cggcggattt cgacctgaac cggttcgccc
     2641 atcgcaccgt ctggaatgat gcggccagcc gcatcgaaat gcacctcgtc agttcgtgcg
     2701 atcaggtcgt gctggttgcc ggccagccga tacacttcac ggcgggtgag accattcaca
     2761 ccgagaacag ctataaacac acgccagagg ccttcctgga gatcacaggg gccgcggggt
     2821 ggggctgccg ggaaatctgg accgacccga aacagtggtt cgcgctttac ctgctggaac
     2881 cgcgcgcagc ggcctgaccg tcagctgcca accttcagcg tggaaaccag accgcgcagg
     2941 caggcaagaa gcgaaagcgg cgtcagcttg ccggtgcgcg gattctcctc actcggcaca
     3001 ttctcgatgg tcatggtgaa gcatgccgcc gccgcatcca cgcgtatggt atgaatattc
     3061 cgcgtaaccc ccggatccgc ccagatctcc acctgagtgc gtcgcggccc cacaccggcc
     3121 aaggccaagg cggccgccac gttcacgttc gcgggaaatc cggccgcagc atccagcgca
     3181 ttcccccgga acacgcacag gggctctgtc aggccggcca cctggattcc atgccgctcc
     3241 agatacggcg cacccgccaa tccggccggg ggcttgcgcg tcaccatggt gacgctctcc
     3301 atcggtccct ccgccgcggc ccgtaccgca tccaagccaa gcagcgcgcc ggtcgggacg
     3361 ataacgcggg ctccggtcgc ctgggcttgg tgcaccagat gcatgcgaga cagcaaagcc
     3421 ccaaccgaag caggaatgaa gatccggccg gcttcgatcg ccgcccgggc gatcggctca
     3481 aacaccgccg ccggggccgc ctcgacgacg atatcggctt ccgccagtgc cgggatctcc
     3541 accagcttcg gcggacgctg aaaatcggcg agattggcgg cggccttgcc ttgatcccgt
     3601 acagcaacgg cgaccagagt cagcccttcg acgccggcat ccaaagcacg ggcgagcggc
     3661 agtccaatgg cgccgagccc agcgatggcg actgtcctgg tcatgattgc tcccccgctg
     3721 cggctttggc cgccagcgcg cgcgcctgcg ccagatcgtc cggtgtattg atgttgaaga
     3781 aggggtccca cgtaccggcc gcgaaatcaa cccgtcgcgc accaatctcc cccgcgaacc
     3841 gggctacgct gcgtggaccc ggggcggaaa gcagggtccg gagacggtcg cggcaggcga
     3901 ccggccatag cgccaccagg tgatgcgccc ggccgccact gaccacacag gctgggggcg
     3961 gatccaagct ggctgccaac cctgctggca caaagggcgt atcccctggg acgctcagca
     4021 atgcaaacac gccctgatcg gccgcccaat ccagccccgc cagcagcccg gccagcgggc
     4081 cctccccggc gaaacagcca tccggcagga cgggaagacc gaaaccggca aaacgcgccg
     4141 gatctccgtt ggcgctgatg gcgagggcag cggcttccga acccaatgcc gcaataaccc
     4201 gctgcagcat cgtccggccg ccaatatcca acagcggctt gtcgccgccc cccatgcggc
     4261 gggcggtgcc gccagccaga atcagcgcag cgatccgttc ccgtggcatc atcggtgagg
     4321 tatggggcga gcaacgggac tcgaacccgt gacatccagg accacaacct ggcgctctac
     4381 caactgagct atgcccgcca tccaggccgc gctatttgcc cgctcggccg agcaccgtca
     4441 acggcagcat gccagtccgg caaatcggcg atggctgggg agatcacccg gtcaggcatt
     4501 ggccaccagt tcgtccagtg cggggtccca cagccggata ccgccggtga tgccggcctg
     4561 gttcggcacc gtgcgcacat tgggcggcag ttcacccgtc aggcgcttcg cattcccgcc
     4621 gccgatcagc agcacgtcat agttcaccaa cgtctcgatc cagccaatcg ccttgcgtac
     4681 ccgcttgttc cagcgcttgg ctccggccga tttcagcgcc gcattgccga tccattcgtc
     4741 gtaggtcttt ccgcgatgcg aaggatgctg gctgagttca agatggggtg ccggcctgcc
     4801 gtcatcgaac agcgcgaagc ccatgccggt acccagggtg atgacgcact ccagcccctt
     4861 tccggagatc acgccgagac
//
LOCUS       DASSZH010000331         6432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_348537, whole genome shotgun sequence.
ACCESSION   DASSZH010000331 DASSZH010000000
VERSION     DASSZH010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6432
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348537"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..429
                     /locus_tag="VFN42_07150"
     CDS             148..429
                     /locus_tag="VFN42_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HET8996428.1"
                     /translation="MCLGIPGRIIAITDADAALATVEIGGVRRPVNISFILDDRPADA
                     CVGDWVLVHVGFAMSRLDAAEAERTLALLSELGEMQAEMEAVRGETPDA"
     gene            439..1569
                     /gene="hypD"
                     /locus_tag="VFN42_07155"
     CDS             439..1569
                     /gene="hypD"
                     /locus_tag="VFN42_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HET8996429.1"
                     /translation="MKYVDEFRDPAKARALVGEIERLLPRIPTARNRPVQIMEVCGGH
                     THTIFRYGIQQMLPDAVEFVHGPGCPVCVLPMGRVDDCVALAERPEVIFTTFGDAMRV
                     PGSRKSLLQAKADGADVRMVYSPLDALALARKHPDREVIFFGLGFETTMPSTAMTVMQ
                     ARAQGVGNFSLFCNHITIIPTIKAILDAPDLRLDGFLGPGHVSMVIGTRPYAFIARDY
                     GRPITVAGFEPLDLLQSTWMVLRQLAEGRCEVENQYGRVVPPDGNPRALAAVQEVFEP
                     REYFEWRGLGSIDRSGVRIRAAYAAYDAERKFAVPNVTVADPQSCQCGEVLTGAIKPH
                     QCRVFGTACTPQTPLGALMVSSEGACAAYYSYGRRETVPANP"
     gene            1566..2591
                     /gene="hypE"
                     /locus_tag="VFN42_07160"
     CDS             1566..2591
                     /gene="hypE"
                     /locus_tag="VFN42_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HET8996430.1"
                     /translation="MTDRQINLSHGGGGRAMRGLIEDLFLPAFGNTRPDALEDQARLP
                     LEELAALGDRLAFTTDSYVVDPLFFPGADIGALAVAGTVNDLAVGGARPLFLSCAMVI
                     EEGLPLATLQRVADSMRAMAQRAGVAIVTGDTKVVERGAADKLFINTAGIGVIPPGVA
                     PSAASARPGDAVIVSGSLGDHGVAILIARNQLALEAEVASDCQPLHRLVAAMLAASPG
                     VRCLRDATRGGVATVLNEFARSSHVGIRIREDALPIREPVRGACEILGLDPLYLANEG
                     KLVTVVPAGDADRVLAAMRADPAGADAAVIGVVTQDPAEMVVMETAFGGERIVDMLVG
                     EQLPRIC"
     gene            complement(2633..2929)
                     /locus_tag="VFN42_07165"
     CDS             complement(2633..2929)
                     /locus_tag="VFN42_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein OmpA"
                     /protein_id="HET8996431.1"
                     /translation="MKHLLAPCALALGASLALSACTNPYDPGQRAVGGGLLGAGTGAA
                     IGGIAGGGRGAAAGALIGGAGGAIGGAATTPQPPPPDYYQAPPPPPPGYYPPRY"
     gene            complement(2962..3246)
                     /locus_tag="VFN42_07170"
     CDS             complement(2962..3246)
                     /locus_tag="VFN42_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996432.1"
                     /translation="MFSVRSWAFGVAVLAALGVLAAAGPAKADWGHHRGWHGGWHHGG
                     WYRWGPPAVVVAPRAYYYAPPPVVYAPPPVYYAPPPAYYPPGVSFGVTFH"
     gene            3416..4324
                     /locus_tag="VFN42_07175"
     CDS             3416..4324
                     /locus_tag="VFN42_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996433.1"
                     /translation="MKLTIPRTLSGDRSLVLHDLPVELAHAGQGNWRAFRAIGLDPGA
                     LLEIARSWAAELQGVTLPWLCWNVDDDWCLVQQKLVSEIGWTPVIGFDPRAGAPRKRV
                     PGAVVVDFNKALGLPVLYPHFPLEFAFLFTERIAFWHSDLLIWPEKMQRLGALFRELP
                     DGQTAAVWMSPGRRHLLSRRKRFWELVGCTTRAASRDQFEQGCGWWMSFWAHPNMRRG
                     ASARRWYYWDHGGGIYYWKTRAGRTVVALDGRDYQEGHFTKIGNPAYQRFREAGSTDA
                     RRSMSAEIRQNFDLEAACRSLGLPGP"
     gene            4388..>6432
                     /locus_tag="VFN42_07180"
     CDS             4388..>6432
                     /locus_tag="VFN42_07180"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET8996434.1"
                     /translation="MTSSDSTGDTGPIGRNLLRREDRRFLTGSARYGDDLAVAGALHA
                     RFVRSPHAHARIRSIDTAAAQAMPGVVLVVSGADLARWTQPLRLAPPIEGLHPVTIET
                     LPTGKVRFHGDPVACVVATSRSAADDAAEQVVVAYEPLPAVTTMQEAVTQGAPLVDEA
                     VPGNLVSRQSFSAGDPERRFATAHRVVETRFVQHRQTHVPMEPRGCCAIWDAGREHLT
                     MHVGTQVPHPYRTNLAFRLGLTESQVTVICPDIGGGFGQKIALYREELTIAALARELG
                     QPVRWREERGENLLASAHAREQTAQVRAAVDADGAITALSVDITEDFGAYCFYPANYL
                     LRMTVLAITGPYRIADFACDMKVVLTNKCGSAPMRAPMSIASWVMDGTIEAVARELAL
                     DPVAVRRRNMLSAAELPWTMAFGPVLEDVTPRETLDAALAAFDVPEFRARQAADRARG
                     VYRGMGLCCLVEANTYGSAFYRASGIPGSGHEAGWVKVAPSGAVDVSVGLMGSGQGYE
                     TALAQAAAEGLGVSPDVVRVHLGNTDIAPYGMGSRGARGGTAGSSVLLLAGGTLQRKA
                     CAIAAALLGLNSGDELRLWDGRVQRMIGGDWTDAGLTLNDIARVAYADPLRLPEGMEP
                     GLEAHRAYDPPPLTFSNATHACEVVVDVETGAVRVERYIVAEDCGRVLNPAIVAGQQ"
BASE COUNT          973 a         2160 c         2331 g          968 t
ORIGIN      
        1 atgcctggag cttagcggat tccggggcaa agcgcgatgg gccgccgccg cgctggccat
       61 gcggccggtc ctcgggcaca atgcggaccc gtcgtttgct ggccggtggc ggcgccgctg
      121 ccagcccacg ccgcaggaga ctgcgtcatg tgcctgggca ttcccggccg gatcattgcc
      181 atcaccgatg ccgacgccgc gctggcgacc gtggaaatcg gcggggtccg ccggccggtc
      241 aacatcagct tcatcctgga tgaccgccct gcggatgcct gcgtcggcga ctgggtgctg
      301 gtgcatgtcg gcttcgccat gagccgcctg gatgcagcgg aagcggagcg cacgctggcg
      361 ctgctgtcgg agctgggcga gatgcaggcc gaaatggagg cggtgcgcgg cgagactccg
      421 gatgcctgag gcacgcgcat gaaatacgtc gacgaattcc gcgatcccgc caaggcccgc
      481 gcgctggttg gcgagatcga gcgcctgctg ccgcgcatcc ccacggcgcg aaaccggccg
      541 gtgcagatca tggaagtgtg cggcgggcac acccacacga ttttccgcta cggcatccag
      601 cagatgctgc ccgacgcggt ggagttcgtg cacggacccg gctgcccggt ctgcgtgctg
      661 ccgatgggac gcgtggatga ttgcgttgcg ctggccgagc ggccggaggt gatcttcacc
      721 acgttcggcg acgcgatgcg tgtgcccggt tcgcgcaaga gcctgttgca ggcgaaggcg
      781 gacggtgccg acgtgcgcat ggtgtattcg ccgctcgatg cactggcact cgcacgcaag
      841 catccggacc gcgaggtgat cttcttcggc ctcggtttcg aaacgacgat gccgagcacc
      901 gcgatgacgg tgatgcaggc tcgggcgcag ggggtgggga atttctcact gttctgcaat
      961 cacatcacca tcatcccgac gatcaaggcg atcctggatg cgcccgatct gcggctggac
     1021 ggtttcctcg ggcccggcca tgtcagcatg gtgataggca cgcggccata cgcgttcatc
     1081 gcccgcgact acggacggcc gatcacggtc gccggattcg aaccgctcga cctgctgcaa
     1141 tccacctgga tggtgctgcg gcaattggcc gaggggcgct gtgaggtgga gaaccagtat
     1201 ggccgcgtgg tgccgccgga cggcaatccg cgtgcgctgg ccgcggtgca ggaggtgttc
     1261 gaaccccggg aatacttcga atggcgcggg ctgggttcga tcgaccgttc cggcgtgcgc
     1321 atccgggcgg cgtacgcggc gtacgatgcg gagcgcaagt tcgccgtgcc gaacgtcacc
     1381 gtggccgatc cacaatcctg ccagtgcggc gaggtcctga ccggcgccat caagccgcac
     1441 cagtgcaggg tgttcggcac cgcctgcacg ccgcagacgc cgctgggtgc gctgatggtg
     1501 tccagcgaag gcgcctgcgc cgcgtattac agctacggcc ggcgcgagac ggtgccggcg
     1561 aacccatgac ggatcgccag atcaacctgt cgcatggcgg cggcggcaga gcgatgcgcg
     1621 ggctgatcga ggacctgttc ctgcccgcct tcggcaacac ccgtcccgac gcgctggaag
     1681 accaggctcg cctgccgctg gaagagctgg cggcgctggg cgaccggctt gccttcacca
     1741 cggacagcta cgtggtggat ccgctgttct tccccggcgc cgatatcggc gcgctggcgg
     1801 tggccggcac ggtgaacgac cttgcggtcg gcggcgcgcg cccgctgttc ctgtcctgcg
     1861 ccatggtgat cgaggaaggg ctgccgctcg ccaccttgca gcgtgtcgcg gacagcatgc
     1921 gcgccatggc gcaacgcgcc ggcgtggcga tcgtcaccgg cgacaccaag gtggtggaac
     1981 gcggtgcggc ggacaagctg ttcatcaaca ccgccggcat cggtgtcatt ccgcccggtg
     2041 tcgcgccttc cgcagccagc gcccgccccg gtgacgcggt gatcgtcagc ggcagcctgg
     2101 gcgatcacgg cgtggcgatc ctgatcgcgc gcaaccagct tgcgctggaa gcggaagtcg
     2161 ccagcgactg ccagccgctg caccggctgg tcgccgcgat gctggcggcc tctcccggcg
     2221 tgcgatgcct gcgcgatgcg acgcgcggcg gcgtggcgac ggtgctgaac gaattcgccc
     2281 gcagttcgca tgtcggcatc cgcatccggg aagacgcgct gccgatccgc gaaccggtgc
     2341 gcggcgcctg cgagatcctc gggctggatc cgctctacct ggcgaatgag ggcaagctgg
     2401 tcaccgtggt gccggcgggc gacgcggatc gcgtgctggc ggcgatgcgc gccgacccgg
     2461 cgggcgcgga tgccgcggtc atcggcgtgg tgacacagga tccggcggag atggtggtga
     2521 tggagaccgc gttcggcggc gaacgcatcg tcgacatgct ggtcggcgag caactgccgc
     2581 ggatctgctg agacggcgag gcggctatgc agccgcctcg cccgggatca gcctagtagc
     2641 gcggcggata atagcccggc gggggcggcg gcggtgcctg gtagtagtcg ggcggcggcg
     2701 gctgcggcgt ggtggccgcg ccgccgatcg ccccgcccgc gccaccgatc agcgcgcccg
     2761 cggcggcgcc gcgcccgccg cccgcgatgc cgccgatcgc ggcgccggtg ccggcgccta
     2821 gcaggccgcc gccgacggcg cgctggcccg ggtcataggg gttggtgcag gcggacaggg
     2881 ccagggacgc acccagggcc agtgcgcaag gcgcaagcag atgcttcatg gggaagctcc
     2941 ttcaatcgag aggccggcgg actagtggaa ggtgacgccg aacgatacgc cgggcgggta
     3001 gtaggctggc ggcggggcgt agtagaccgg agggggcgca tacaccaccg gcggtggcgc
     3061 gtagtaatac gcgcgcggtg caaccaccac cgcaggcggt ccccagcgat accagccgcc
     3121 atggtgccag ccgccatgcc agccgcgatg atgaccccaa tcggccttgg ccggcccggc
     3181 ggcggcaagc acgcccagag ccgcaagcac ggcaacgccg aacgcccagc tgcgcacaga
     3241 aaacatcgca accctccttc gcaaccgttg cgagatgacc acgggatcat ggcaggagca
     3301 tgtcatcacg ccatgagatc cttccacgcc aaccggcgcc ggcacggtcc tggcgcagca
     3361 aagctccggc cgctaatttg cccgccgcgt gcgggcggga catccggggg tcctggtgaa
     3421 gctgaccata ccaagaacac tctccggcga ccgctcactg gtcctgcacg accttccggt
     3481 ggaactggcg catgccgggc aaggcaactg gcgggccttt cgggcgatcg ggctcgaccc
     3541 gggggcgctg ctcgagatcg cgcgttcatg ggcagcggaa ctgcaaggcg tcacgctgcc
     3601 ctggctgtgc tggaacgtgg acgacgattg gtgcctggtg cagcagaagc tggtcagcga
     3661 gatcggctgg acgccggtga tcggattcga tccgcgggcc ggcgcgccac gcaagcgggt
     3721 ccccggcgcg gtggtggtgg atttcaataa ggcgctggga ttgccggtgc tgtacccgca
     3781 tttcccgctg gagttcgcgt ttctgttcac cgagcggatc gcgttctggc actccgatct
     3841 gctgatctgg ccggaaaaga tgcaacggct tggcgcgctg ttccgcgaac tgccggatgg
     3901 ccagacggcg gcggtgtgga tgtcgccggg ccggcgccac ctgctgtcca gacgcaagcg
     3961 gttctgggaa ctggtgggtt gcacgacccg tgcagcgagc cgcgatcagt tcgagcaggg
     4021 ctgcggctgg tggatgagct tctgggcaca tcccaacatg cgccgcggcg catccgccag
     4081 gcgctggtac tactgggacc acgggggcgg catctattac tggaagacgc gcgccggccg
     4141 gaccgtggtg gcgctggacg gccgggacta ccaggaaggc catttcacca agatcggcaa
     4201 cccggcgtac cagcgctttc gcgaagccgg ctccacggat gcccggcgat cgatgagcgc
     4261 cgagatcaga cagaatttcg acctcgaggc agcctgccgc tccttgggtc tgcccggccc
     4321 ctgagcggaa gccggcggcc gggcggcgaa tattccaccc gcgcggccgg cccgctacac
     4381 agcggacatg accagttccg acagcaccgg cgacacgggt ccgatcggcc gcaacctgct
     4441 gcgccgcgag gatcgccgct tcctgaccgg ctcggcgcgc tatggcgacg atctggccgt
     4501 ggccggcgcg ctgcatgccc ggttcgttcg ctcgccgcat gcgcacgcac gcatccgctc
     4561 catcgacacc gccgccgcgc aggcaatgcc gggcgtcgtg cttgtggtca gcggcgcgga
     4621 cctggcgcgc tggacacagc cattgcgcct cgccccgccg atcgaagggc tgcacccggt
     4681 caccatcgag acactgccca ccggcaaggt gcgtttccac ggtgatccgg tggcctgcgt
     4741 ggtcgccacc agccgcagcg cggcggatga cgccgcggag caggtggtgg tggcgtacga
     4801 accgctgccg gcggtcacca cgatgcagga agccgtcacg cagggcgcgc cgctggtgga
     4861 cgaagcggtg cccggcaacc tggtgtcccg gcaaagcttc agcgccggcg atcccgagcg
     4921 gcgcttcgcc accgcgcatc gcgtggtgga gacccgcttc gtgcagcacc ggcaaaccca
     4981 cgtgccgatg gaaccgcgcg gctgctgcgc gatctgggat gccgggcgtg agcacctgac
     5041 catgcatgtc ggcacccagg tgccgcaccc ataccgcacc aatcttgcct tccggctcgg
     5101 cctgacggaa tcgcaggtca ccgtgatctg cccggatatc ggcggcggct tcggccagaa
     5161 gatcgcgttg taccgcgagg aactcaccat cgccgcactg gcccgcgagc tgggccagcc
     5221 ggtgcgctgg cgcgaggagc gcggtgagaa cctgctggca tccgcgcatg cccgcgagca
     5281 gaccgcgcag gtgcgcgcgg cggtggatgc ggacggcgcc atcaccgcgc tgtcggtcga
     5341 tatcaccgag gatttcggcg cctactgctt ctacccggcg aactacctgc tgcgcatgac
     5401 cgtgctggcg atcaccggcc cgtaccgcat cgccgatttt gcctgcgaca tgaaggtggt
     5461 gctgaccaac aaatgcgggt cggcgccgat gcgcgcgccg atgtcgatcg ccagttgggt
     5521 gatggacggc acgattgagg cggtggcacg cgaactggcg ttggacccgg tcgcggtgcg
     5581 ccgccgcaat atgctcagcg ccgccgaact gccctggacc atggccttcg ggccggtgct
     5641 ggaagacgtg acgccgcggg aaacgctgga cgccgcgctg gccgccttcg acgttccgga
     5701 attccgcgcc cgccaggccg ccgaccgcgc ccgcggcgtg tatcgcggca tgggcctgtg
     5761 ttgcctggtg gaggccaata cctatggctc tgcgttctac cgcgcgtccg gcatccccgg
     5821 ttccggccac gaggccggct gggtgaaggt ggcgccgagc ggcgcggtgg atgtcagcgt
     5881 cgggctgatg gggtcgggcc agggctatga gaccgcgctg gcccaggcag ccgccgaggg
     5941 gctgggcgtg tcgcccgatg tggtgcgggt gcacctgggc aacaccgaca tcgcgcccta
     6001 cggcatgggc agccgcggcg cgcgtggcgg caccgccggc agctcagtgc tgctgctcgc
     6061 cggcggcacc ctgcaacgca aagcctgcgc catcgccgcc gccctgctcg gcttgaactc
     6121 cggcgacgaa ctgcggctgt gggacggccg ggtgcagcgg atgatcggcg gcgactggac
     6181 cgatgccggc ctgacgctga acgacatcgc ccgcgtcgcc tatgccgacc cgctgcgcct
     6241 gccggagggc atggagccgg ggctggaggc gcaccgtgcg tacgatccgc cgccgctgac
     6301 cttctccaac gccacgcatg cctgcgaggt ggtggtggat gtggagaccg gcgcggtgcg
     6361 ggtggagcgc tatatcgtcg ccgaggattg cggccgcgtg ctgaacccgg cgatcgtcgc
     6421 cggccagcag ca
//
LOCUS       DASSZH010000332         2777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1576195, whole genome shotgun sequence.
ACCESSION   DASSZH010000332 DASSZH010000000
VERSION     DASSZH010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576195"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VFN42_07185"
     CDS             complement(<1..699)
                     /locus_tag="VFN42_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996435.1"
                     /translation="MRRRHFLAAATGIGLLRTRAALAGPRDWLDAEVNGMHYKVLPPD
                     AAAPPPHPVVLYLHQLDMGDWPDGLLQQVNPWFNTAAFRSRHPCIVVMPLLDQKKDPA
                     GHTINFGGDRHGHVGEDNAMAALRQVMAANPVDPRRIYITGNSLGGMGTWAMLLAYNR
                     LTGRQGRIFAAGMPLAGTDRSANPVQAARTLRHVPVWAFHGAHDTKVSPAWDRHMARL
                     LSGARTFRYTEYGDL"
     gene            complement(699..1874)
                     /locus_tag="VFN42_07190"
     CDS             complement(699..1874)
                     /locus_tag="VFN42_07190"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET8996436.1"
                     /translation="MPLTGLKVLDLTRVLSGPFCTALLGDLGADIIKIESPGGDSVRN
                     QGAIKDGLSWYFTQFNRNKRSVRLDLRQPAAKDILARLIAQSDVLVENFRPGVLARLG
                     FDASRMEALNPRLITCSINGFGSTGPYRDRPAFDFIAQAMSGFMSVNGGPDDPPLRSG
                     LPISDLVAGLYAALSITASVLHARATGQGQRTEVSLTNGLVSLLAYIATNYFATGTVP
                     PRSGNDHPIAAPYGLFPTRDGQIALAPADDAFFRRLADAISAPHLKTDPLYATQAARV
                     ANRARINAIVGGKLAEQTTGYWVETLNRAGVPCGPVLGVEGVFEDPQILAQEMVMDVE
                     QPGYGPVRMLGFPMKLSGTPCRVRRPAPALGEHTGEVLAELGYTTAQIEAWRREELI"
     gene            2068..2412
                     /locus_tag="VFN42_07195"
     CDS             2068..2412
                     /locus_tag="VFN42_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996437.1"
                     /translation="MTRFTSLLVASGLAILPISAFAQTTATDSKPAAPVAAATMAPAA
                     ATAEPATKAPAAPAAATTASTMAATPAKPVEKMAAKPQHHHHHVRAMKVKHTKMTAPT
                     GVEQKAPDQSKS"
     gene            complement(2470..>2777)
                     /gene="gatB"
                     /locus_tag="VFN42_07200"
     CDS             complement(2470..>2777)
                     /gene="gatB"
                     /locus_tag="VFN42_07200"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459936.1"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HET8996438.1"
                     /translation="DLIADSTINGKIAKDVFESMVETGKDAAAIVDERGLRQVTDTGA
                     IDAAVDRILAANADKVAEYRSGKDKLFGFFVGQTMKAMAGKGNPALINETLKRRLAG"
BASE COUNT          455 a          950 c          923 g          449 t
ORIGIN      
        1 caggtcgcca tattcagtat agcgaaaggt gcgggcgccg gacagcagcc gggccatgtg
       61 ccggtcccag gcgggggaga ctttcgtgtc gtgcgcgccg tggaacgccc agaccggcac
      121 gtggcgcagc gtccgcgccg cctgcacggg gttggcggac cgatccgtgc cggccagcgg
      181 catcccggcg gcgaagatcc ggccctgccg cccggtcagg cggttgtacg ccagcagcat
      241 cgcccaggtc cccatgccgc cgagggaatt tcccgtgatg tagatacgcc gcggatcaac
      301 cggatttgcc gccatcacct gccggagcgc ggccatggca ttgtcctcgc cgacgtggcc
      361 gtgcctgtcg ccgccgaagt tgatggtgtg gccggccgga tcctttttct ggtccagcag
      421 cggcatcacg acgatgcagg gatgccggct tcggaaggcg gcggtgttga accaagggtt
      481 gacctgctgc agcaggccgt ccggccagtc ccccatgtcc agttggtgca ggtacagcac
      541 caccgggtgg ggcggcggcg ccgcagcgtc cggtggcagc accttgtagt gcatcccgtt
      601 cacctcggca tcgagccagt cccgcggtcc ggccagcgcg gcccgggtgc gcagcaggcc
      661 gataccggtc gccgcagcca gaaagtgccg ccgccgcatc agatcagctc ctcccgccgc
      721 caggcctcga tctgcgccgt ggtgtagccg agttcggcga gaacctcgcc ggtatgctcg
      781 cccaacgccg gggccggacg gcggacccgg catggcgtgc cagacagctt catcgggaag
      841 cccagcatgc ggaccgggcc gtagccgggc tgctcgacgt ccatcaccat ctcctgcgcc
      901 aggatttgcg ggtcttcaaa cacaccctcc acgcccagga ccgggccaca gggcacgccg
      961 gcccggttca gcgtttccac ccagtacccg gttgtctgtt cggccaattt tccgccgacg
     1021 atggcgttga tgcgtgcccg gttggcgacg cgcgccgcct gcgtggcata gagcgggtcg
     1081 gtcttcaggt gcggcgcgct gatggcgtcg gccagccggc ggaagaacgc gtcgtcggcc
     1141 ggggcgaggg cgatctggcc atcccgcgtc ggaaacaggc catacggcgc ggcgatcggg
     1201 tggtcgttgc cgctgcgcgg cggcaccgtc ccagtggcga aatagttcgt ggcgatatag
     1261 gccagcaggc taaccagccc gttggtcagg ctgacctcgg tgcgctggcc ctggccggtg
     1321 gcgcgcgcat gcaggacgct ggcggtgatg ctgagcgcgg catacaggcc cgcgacaagg
     1381 tcgctgatcg gcaggccgga ccgcagcggc gggtcgtcgg ggccgccatt gacgctcatg
     1441 aagccgctca tggcctgggc aatgaagtcg aaggccgggc ggtcgcggta gggaccggta
     1501 ctgccgaacc cgttgatgga gcaggtgatg aggcgggggt tcaacgcctc catccgactc
     1561 gcatcgaagc ccaggcgggc cagcacgccg ggccggaaat tctccaccag cacgtcggat
     1621 tgcgcgatca ggcgtgccag gatgtccttc gccgccggct gacggagatc gaggcggacc
     1681 gagcgcttgt tgcggttgaa ctgcgtgaaa taccaggata gcccgtcctt gatcgcgccc
     1741 tggttgcgca ccgaatcgcc ccccggcgac tcgatcttga tgatgtcggc gcccaggtcg
     1801 cccagcaggg cggtgcagaa ggggcccgac agcacacggg tcaggtcaag gactttcagg
     1861 ccggtcagcg gcatcggcgt ttcctgttct ggctggcgca ctgtcccacc cgtcggggca
     1921 gcctgccaag atggcggatt tttaatacga tttagcatca ttgccgcgat ttcgccgcgg
     1981 atgcagcgtg cgagcgtcat aatctcccgg ctatatcaat cgcagccggg aacgacagat
     2041 ttcctccccg gtcgaaagga accgacgatg acacgtttca cgagcctgct ggttgcgtcc
     2101 ggcctggcga tcctgccgat cagcgccttc gcccagacga ccgccaccga cagcaagccg
     2161 gccgcgccgg ttgccgctgc aacgatggcg ccggccgctg cgacggccga accggcaacc
     2221 aaggcacctg cggctccggc tgccgccacg acagcctcga ccatggccgc gaccccggca
     2281 aagcccgtcg agaaaatggc cgcgaagccg cagcaccatc accaccatgt cagggccatg
     2341 aaggtgaagc acacgaagat gaccgcgccc accggcgtgg agcagaaggc accggatcag
     2401 tcgaagtcct gacgaattcg acggagcctg gccggaaacg ccaggctctc cagccagcct
     2461 ccctggcggt cagccggcca ggcgtcgctt cagcgtctca ttgatcagcg ctggattgcc
     2521 cttgccggcc atcgccttca tcgtctgccc gacgaagaac ccgaacagct tgtctttccc
     2581 cgaccggtat tccgccacct tgtcggcgtt cgcggccagg atcctgtcca ccgccgcatc
     2641 gatcgcgccg gtatcagtca cctgccgcaa cccgcgttca tcgacgatcg ccgcggcatc
     2701 cttgccggtc tccaccatcg actcgaatac gtccttggca atcttgccgt tgatcgtgct
     2761 gtcggcgatc aggtcca
//
LOCUS       DASSZH010000333         4001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1610216, whole genome shotgun sequence.
ACCESSION   DASSZH010000333 DASSZH010000000
VERSION     DASSZH010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610216"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VFN42_07205"
     CDS             complement(<1..371)
                     /locus_tag="VFN42_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET8996439.1"
                     /translation="MTEPVAQVIDAARAFLGERITTNATLREHHSHGQDTQPPVLPDA
                     VAFVETTEEASRVLALCNEHHVPVVAWGAGTSLEGHVTPVRGGITLDLSRMTRIISVS
                     QPDMDCRIEAGVTREQLNTHLR"
     gene            551..754
                     /gene="rpmF"
                     /locus_tag="VFN42_07210"
     CDS             551..754
                     /gene="rpmF"
                     /locus_tag="VFN42_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003620805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET8996440.1"
                     /translation="MAVPKRKTSPSRRGMRRSHEALPREAHAECPNCGELKRPHHVCS
                     HCGFYDGREVVAAGKALKGAVRA"
     gene            786..1853
                     /gene="plsX"
                     /locus_tag="VFN42_07215"
     CDS             786..1853
                     /gene="plsX"
                     /locus_tag="VFN42_07215"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HET8996441.1"
                     /translation="MTAEYALAVDAMGGDHAPAMVIDGLEIAVERHPGARFLLLGDEA
                     QLTALLARHKRARSACTIRHTADVIPNEMKPTAALRARNASMRLAIDAVAAGEASGVV
                     SAGNTGALLALSKIVLKSLPGIDRPAMAAIGPSARGDVVMLDLGANVQCDARNLVQFA
                     VMGDVFARTVLGLTSPSIGLLNVGSEELKGDDTVRQAAEILRASPIAPQFHGFVEGHD
                     IAAGTTDVVVTDGFTGNVALKTGEGALKLIGDLMRQVFTSSVSAKLGYLLARSSLDRL
                     REWLDPRRYNGAVMVGLNGVVVKSHGGTDSQGFAHAVDVAMDMVVHRFNEQIREGLAR
                     IALDTLMAQEKNGAGQRLVTG"
     gene            1853..2842
                     /locus_tag="VFN42_07220"
     CDS             1853..2842
                     /locus_tag="VFN42_07220"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003623931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HET8996442.1"
                     /translation="MTDRTPMPPRSILAGIGAYLPQRILTNEELARTVETSDAWIQER
                     TGIRQRHIAGPHETAAFMGTEAARAALADARAEPAEVDAIILATSTPDQAFPATALRV
                     QAALGVPGGFGFDLAAACAGFLYALSMADSLIHSGQARGVLVIGSEVYSRILNWQDRG
                     TCVLFGDGAGAVFLRAGEGRGTIDRGILSTHLHAQGSLGDILYVDGAVGQPDKSGHLV
                     MKGQEVFRHAVTRLAEAVDEALAANGLTRRDIDWLVPHQANLRIIESMGRKLGLPSER
                     VVVTVDRHANTSAASVPLALAEAHQDGRIRKGDLVLLEALGGGLTWGSALVRM"
     gene            2963..3781
                     /locus_tag="VFN42_07225"
     CDS             2963..3781
                     /locus_tag="VFN42_07225"
                     /inference="COORDINATES: protein motif:HMM:NF020478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity EipB family protein"
                     /protein_id="HET8996443.1"
                     /translation="MRFRAVAAALILAASIPGLAPAAGLPITAPVSTKGLASHHALYT
                     LTLDKAPGGDVVAAHGTMGYEVMDACDGWATRQRLNIIVTNADGQDIRMVSDYATWES
                     IDGLKFRFHMKQTTEGAVTSQTDGYASLDRRGGPGVVHYTLPKPKVVKLPPGTLFPMT
                     HTATLLAGAREGKRFMALPLFDGTDDNGAEDSSVVVVDVRKPFQTRWPALSVMPSARV
                     RLAFFDRKPDTMLPNYEVGMRYWENGVADDMQMDFGDFVMDGKMTEFRLLPHKC"
BASE COUNT          623 a         1354 c         1381 g          643 t
ORIGIN      
        1 cgcaggtggg tgttgagctg ctcgcgcgtc acccccgcct cgatgcggca gtccatgtcg
       61 ggctggctga cggaaatgat ccgggtcatg cgcgacaggt cgagggtgat gccgccgcgc
      121 accggggtga cgtggccctc cagcgaggtg ccggcgcccc aggccaccac cggcacgtgg
      181 tgctcgttgc acagggcaag cacgcgactg gcttcctcgg tggtctccac gaaggcgacc
      241 gcgtccggca gtaccggcgg ctgggtgtcc tggccgtgcg agtgatgttc ccgcagcgtg
      301 gcattggtgg taatccgctc accgaggaag gcgcgggcgg catcgatcac ctgggcaacc
      361 ggttcggtca tttcgtgtcc ttgtgaagct cggcggcgga ggattggacc cggagacggg
      421 acgtggcaag cggcggcctt gcgacccccg gccccctctg ctagacggcc cccctcgcgt
      481 tgtttccatc cggtgaatgc ccctcgcacc cgcggggcca cagctattaa ggattgaggg
      541 gcactgtccc atggccgttc cgaagagaaa gacgtcgcct tcccgccgcg gcatgcgccg
      601 cagccatgag gccttgccgc gtgaagccca cgccgaatgc ccgaattgtg gcgaactgaa
      661 gcgcccgcac cacgtctgca gccattgcgg cttctatgac gggcgtgagg tggtcgcggc
      721 cggaaaggcg ctgaagggcg ctgtccgggc ctgacgccgg gcgcccgcgt gtcggcgtcc
      781 ttaccttgac cgcagaatac gcgctggccg tagatgccat gggcggcgat cacgcccccg
      841 ccatggtgat cgatgggctg gagattgccg tcgaacggca tcccggcgcg cgttttctgc
      901 tgctcggtga cgaggcacag ctcaccgctc tgcttgcccg gcacaagcgc gcccgttcgg
      961 cttgcacgat tcgacatacc gcggatgtca tcccgaatga gatgaagccc acggcggcgt
     1021 tgcgcgcccg caacgcctcg atgcgcctgg cgatcgacgc cgtggccgcg ggcgaggcct
     1081 ccggcgtggt gtccgccggc aacaccggcg cgctgctggc gttgtccaag atcgtgctga
     1141 aatcgctgcc cggcatcgac cgcccggcca tggcggcgat cggcccgtcc gcacggggcg
     1201 acgtggtcat gctggacctg ggcgccaatg tgcagtgcga cgcccgcaac ctggtgcagt
     1261 tcgcggtgat gggcgacgtg ttcgcccgca ccgtgctggg gctcacctca ccctccatcg
     1321 gcctgctgaa cgtcggctca gaggaactga aaggcgacga caccgttcgg caggcggcgg
     1381 aaatcctgcg cgccagtccg atcgcgccgc agttccacgg cttcgtcgaa ggccacgaca
     1441 ttgccgccgg caccaccgac gtcgtggtca ccgacggctt caccggcaac gtcgcgctga
     1501 agaccggcga aggcgcgctg aagctgatcg gcgacctgat gcgccaggtt ttcaccagca
     1561 gcgtctccgc gaaactggga tacctgctgg cgcgctccag cctggaccgg ctgcgcgaat
     1621 ggctcgatcc gcggcgctac aatggcgcgg tgatggtcgg gctgaacggc gtggtggtga
     1681 agtcgcacgg tggcaccgat tcgcagggct ttgcccatgc cgtggacgtc gcgatggaca
     1741 tggtggtcca ccgcttcaac gagcagatcc gcgaagggct tgcacgaatc gcgctggata
     1801 cgctgatggc ccaggaaaag aatggggccg gccagcgact cgtcaccggc tgatgacaga
     1861 caggacaccg atgccccctc gctccatcct tgccggtatc ggcgcctacc tgccgcagcg
     1921 catcctcacc aatgaggaat tggcccggac ggtggaaacc tccgacgcat ggatccagga
     1981 gcgcaccggc atccgccagc gccatatcgc cggcccgcac gagacggccg ccttcatggg
     2041 caccgaggcg gctcgtgccg ccctggccga tgcccgggcc gaaccggccg aggtggacgc
     2101 gatcatcctg gccaccagca cgcccgacca ggcatttccg gccaccgcgc tccgggtaca
     2161 ggccgcgctg ggcgtgccgg gcggcttcgg cttcgacctg gccgctgcct gtgccggctt
     2221 cctctacgcg ctgtcgatgg ccgattccct gatccactcc ggccaggcgc ggggggtgct
     2281 ggtgatcggc agcgaggtct attcgcgcat cctgaactgg caggaccgcg gcacctgcgt
     2341 gctgttcggc gatggtgccg gcgcggtgtt cctgcgcgcc ggcgagggcc gcggcacgat
     2401 cgaccgcggc atcctgtcca cccacctgca cgcccaaggc tcgctgggcg atatcctgta
     2461 tgtcgatggg gcggtcggcc agccggacaa gtccggccac ttggtgatga aaggccagga
     2521 agtgttccgc cacgcggtga cccggctggc cgaggccgtg gatgaggcct tggccgccaa
     2581 cggcctgacc cgccgcgaca tcgactggct ggtgccgcac caggccaatc tgcggatcat
     2641 cgaaagcatg ggccgcaagc ttgggctgcc cagcgagcgg gtggtggtga cagtggaccg
     2701 gcatgcgaac acctcggccg cgtcggtgcc gctggcgctg gccgaggcgc atcaggacgg
     2761 ccgcatccgc aagggcgacc tggtactgct ggaggcgttg ggtggtggat tgacctgggg
     2821 ctccgcgctg gtgcggatgt agccgccggc cagggcgtgc cggcgcttct gccctggtgg
     2881 attcccgtcc gctcacccta tggttgcccc agccttcccg gtccgggatg aaccgggctg
     2941 ccttctaccc gcggagaccc tcatgcgctt tcgtgccgtt gcggccgccc tgattctcgc
     3001 tgcctcgata ccgggtctcg ctccagccgc cggactgccg ataaccgcgc cggtcagcac
     3061 caaaggactg gcctcgcacc acgcgcttta cacgctcacg ctggacaagg cgccgggcgg
     3121 ggacgttgtc gcggcgcatg gcaccatggg ctatgaggtc atggatgcct gcgacggctg
     3181 ggcgacccgg caacggctga acatcatcgt caccaatgcc gatggccagg acatccggat
     3241 ggtgtcggac tatgccacct gggaatcgat cgacgggctg aaattccgct tccacatgaa
     3301 gcagaccacc gagggcgcgg tgaccagcca gaccgacggc tatgcctcgc tggaccgccg
     3361 cggcgggccg ggcgtggtcc attacacgct gccgaagccg aaagtggtga agctgccgcc
     3421 cggcacattg ttcccgatga cacacaccgc cacgctgctt gccggcgcgc gtgaggggaa
     3481 gcgcttcatg gcgctgcccc tgttcgacgg cactgacgat aacggggcgg aggacagttc
     3541 ggtggtggtg gtcgacgtgc ggaagccgtt ccagacgcga tggccggcat tatccgtcat
     3601 gcccagcgcg cgcgtccggc tggcgttctt cgaccgcaag cccgacacca tgctgccgaa
     3661 ctacgaggtg ggcatgcgct actgggaaaa tggcgtggcg gacgacatgc agatggattt
     3721 cggcgacttc gtcatggacg gcaagatgac ggaattccgc ctgctgcccc acaaatgctg
     3781 agccggcgcg ggccgtcatt gccgccgcag cggcggccct gggatcagcc ggcgcgggac
     3841 cctgctgcaa tccgtgccaa gcccgcgagc gccgccgcgg ataccaggtg catccgtcag
     3901 cgcgctctgc tgaccgtgta atccgcgaca ttggcgaggg cgcggcgcca ggcgctgtcc
     3961 gggaagaccg ccagcgccgc cttggcagac tcggcgaacc g
//
LOCUS       DASSZH010000334         2411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_178517, whole genome shotgun sequence.
ACCESSION   DASSZH010000334 DASSZH010000000
VERSION     DASSZH010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178517"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VFN42_07230"
     CDS             complement(<1..555)
                     /locus_tag="VFN42_07230"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8996444.1"
                     /translation="MTAKPFVIALEEHFQIPELVATYPQLDATRAPPIAERLEDVGAL
                     RIKEMDEAGIDLQVLSHSAPAVQKIADAETAIRLAKLANDRLAETVRAHPHRFAGFAT
                     LPTQDARAGADELERAVTQLGFKGAMIHGLTNGHFHDEKQFWPIYERAQALDVPIYFH
                     PALPSQAVVEAYYKEYAQQIPVILR"
     gene            complement(625..>2411)
                     /locus_tag="VFN42_07235"
     CDS             complement(625..>2411)
                     /locus_tag="VFN42_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996445.1"
                     /translation="FIQDKGLAQARMVDNLIVGVSPAGLMTGSGADKVRMSGNVITDR
                     PGVADEAGRLYFLTAHSPAVRRAVPAGDGAGFPLTARYEFVFPHGGQPRPVAGRPDVG
                     AYAFVPGQRIPRPPVIRLHAEQPKVAYNAPARLTWTASDAEQCRASGGWSGDEPVSGT
                     FTTQSLTQDTGFALTCSGPSGRSHASIMITVGESPQAAALGTYRWQRIAHSALERLCP
                     SRTAYADIYGTTGCEAKQGSATGVYVQPAQEWYLLGGGGTNGYYGNEVYGFSLATLRP
                     QRVTDPTHISAAREYMADDPSDKLHLKTCDGILHLKDGGFAPAPRGIVGQAAYDPRNR
                     KIIVGPWGVVQGIGDCSGSTLGRFATDQWSFDPFTHQWSLLAPADERFGSTIPSTWFL
                     DPDSGIAYDADGSRTGATRGAWLINYAGTPPHAELVNGHWPYAVSIGPVAVDTTTHHA
                     LQLIQQTNAMPPRIAVYDLRRLMGLYDPAGGREAADTSYRPDTSWTVTGDTGILAAAH
                     PALTFNPKLGMFVAWAGGDAVYFLLPDYAAKTLSILVKHIGGGATPQGDYAPDGWFTY
                     LPDHDAYLVFAGMQQDFTLLLPTGARMP"
BASE COUNT          397 a          761 c          834 g          419 t
ORIGIN      
        1 acgcaggatc accgggatct gctgggcgta ttccttgtag taggcctcca ccaccgcctg
       61 gctgggaagc gcgggatgga agtagatcgg cacgtccagc gcctgggcgc gctcgtagat
      121 cggccagaac tgcttctcgt cgtggaaatg gccgttggtg aggccgtgga tcatcgcacc
      181 cttgaagccg agctgggtga cggcgcgctc cagctcgtcg gcgccggcgc gcgcgtcctg
      241 ggtgggaagc gtcgcgaagc cagcgaagcg gtgcggatgc gcgcgtaccg tctccgccag
      301 ccggtcgttg gcgagcttcg ccagccgtat cgcggtttcc gcatcggcga tcttctgcac
      361 cgccggtgcg gagtgggaca gcacctgcag gtcgatgccg gcttcatcca tttccttgat
      421 gcgcagcgcg cccacatctt ccagccgttc ggcgatcggc ggcgcgcggg tggcgtccag
      481 ttgcggatag gtggcaacca gttccggaat ctggaaatgc tcctcgagcg cgatgacgaa
      541 tggcttggca gtcatgggac gtctccctgg gtgatgcggc gcaggcagcc ttgagatcga
      601 aatgcgtgcc gtcaagcgac gcggctatgg catccgggcg ccggtcggca gcagcagagt
      661 gaaatcctgc tgcatgccgg cgaacacgag gtaggcatcg tgatccggca ggtaggtgaa
      721 ccagccgtcc ggcgcgtaat cgccctgcgg cgtcgcgccg ccgccgatat gcttgaccag
      781 gatgctcagg gtcttggccg cgtaatcggg aagcaggaaa tacaccgcgt cgccacccgc
      841 ccaggcgacg aacatcccca gtttcggatt gaaggtcagt gccggatgcg cggcggcgag
      901 aatgccggtg tcgccggtta ccgtccatga cgtgtcgggc cgatatgacg tgtcggctgc
      961 ctcgcgtccc ccggcaggat cgtacagacc catcagccgc cgcaggtcgt aaacggcgat
     1021 ccgcggcggc atggcattgg tctgctggat caattgcagc gcgtggtgcg tcgtggtgtc
     1081 cacggcgacc gggccgatcg acacagcgta cggccagtgt ccattcacca attcggcgtg
     1141 cggcggcgtg ccggcgtaat tgatcagcca ggccccgcgc gtcgcgccgg tcctgctgcc
     1201 gtcggcatcg taggcaatgc cgctgtccgg atcgaggaac catgtggatg gaattgtgct
     1261 gccgaagcgt tcatcggccg gagccagcaa gctccattga tgggtgaacg ggtcgaaact
     1321 ccactgatcg gtggcgaagc gtcccagtgt ggagccgctg caatcgccga tcccctgcac
     1381 aacgccccag ggaccgacaa tgatcttgcg attgcgcggg tcgtacgctg cctggccgac
     1441 gatgccgcgt ggcgccggcg cgaagccgcc gtccttcagg tgcagaatgc cgtcgcatgt
     1501 cttcaggtgc agtttgtcgg acggatcatc cgccatgtat tccctggcgg cggagatatg
     1561 cgtcggatca gtgacccgct gcgggcgcag cgtggcgagg gagaagccat agacctcgtt
     1621 gccgtaatag ccgttcgtgc cgccgccacc cagcaagtac cattcctgcg ccggttgaac
     1681 ataaacgcca gtcgcggaac cctgcttcgc ttcacagccg gtggtgccgt agatgtcggc
     1741 gtacgcggtg cgcgacggac acaggcgttc cagtgccgaa tgggcgatgc gttgccagcg
     1801 gtacgtgccc agcgcagccg cctgtggcga ttctcccaca gtgatcatga tcgatgcatg
     1861 gctgcggcca ctcggcccgc tgcaagtcaa ggcaaatccg gtatcctgcg tgagcgactg
     1921 ggtggtgaat gtgccggata ccggttcgtc gccggaccac ccgccagatg cgcggcattg
     1981 ctccgcatcg cttgcggtcc aggtaagccg ggccggtgcg ttgtaggcga ccttgggttg
     2041 ctcggcgtgc agacggatca ccggcggacg cggaatccgc tgccccggaa cgaacgcgta
     2101 ggcgccaacg tcaggcctgc cggcgacggg ccgcggctgg ccgccgtgcg gaaacacgaa
     2161 ttcgtagcgc gcggtgagcg ggaaaccggc gccatcgccg gccggaaccg cacgccgcac
     2221 ggcgggcgag tgcgcggtca ggaaatagag ccggccggct tcgtcggcga cgcctgggcg
     2281 gtcggtgatc acgttgccgg acatccgcac cttgtccgcg ccgctgcctg tcatcaggcc
     2341 cgcgggggac acgccgacga tcagattgtc caccatgcgg gcctgcgcga gtcccttgtc
     2401 ctggatgaac a
//
LOCUS       DASSZH010000335         5186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_808865, whole genome shotgun sequence.
ACCESSION   DASSZH010000335 DASSZH010000000
VERSION     DASSZH010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808865"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="VFN42_07240"
     CDS             complement(<1..1239)
                     /locus_tag="VFN42_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996446.1"
                     /translation="MPSWWSGGRLMHPEYAISALRLDPLVPVWVIALLGGIALLVVAI
                     GVWRRARGTLLRLAAFAVLLGWLAGPRLVQETWQGLSDIGLLVMDHSDSMKLGDRMQL
                     AEQARAAITAQAGKMPGLELRTINVPEQGDNGTQLFAAINRAMGDIPRARLAGTIAIT
                     DGEISDLPKSTPGGAPLNVLIPAKGEEIDRRLRVIEAPGYGIVGKSVTLKVVVDDQGV
                     RHPNSSADLTIRRDGQPALTESVPVGHPENITVPITRAGPTVIEMSADKLPGEVSTLN
                     NRAVVEINGVRDRLRVLLISGAPAPAVRTWRRLLKSDPSVDLVHFTILRPPDKDDMTP
                     LNQLALIAFPVRELFQEKIGEFDLIILDQFQNRGLLPLPYLANIATRVREGGALLMNV
                     GPEFSGSGGLAETPLGSILPA"
     gene            complement(1247..3934)
                     /locus_tag="VFN42_07245"
     CDS             complement(1247..3934)
                     /locus_tag="VFN42_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019084536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HET8996447.1"
                     /translation="MIFAAPWVLLALIGLPVLWWLLRVTPPAPRSESFPAIRLLLGLH
                     AKEETPARTPWWLMTLRLLAAALVIVALARPVLNAGTEFAGSGPMLLVLDNGWASASD
                     WARRMQAADSMLDRAARAGRQAALLTTAPGETGTLPRITSVMPIASLRSRLAALRPEP
                     WPADRAAAAAALDQWHHAGAAVVYLADGLTDGSDFPRFSEALRHAGSVTELCCAATAA
                     RMLLPPDSNADRLVVRLAQVPQPAPTSAVVLAQTGDGRTLARTPLDLPAGAAIGSAPL
                     MLPPEIRNELARLVLQGSQSAGSVFLLDERYRRRPVGLAAGDAVAANTPFVGSLYFVS
                     RALQPYAELREGSLDTLLKRKLSVLILADDPLPAGPERAAVAKWVEQGGLLIRFAGSR
                     TAEQPIGETDPLMPVPLLQGDRALGGAMSWSKPAGLAPFPPNSPFAGLDAPADVTVNR
                     QVLAQPSADLAAHTWAALKDGTPLVTEATKGAGRIVLFHVTANVDWSNLPLSGLFVDM
                     LRRLVSLSVGVKSEPPHTLLSPAETLDGFGMLGAPPQSATGLRADEFGQAMISPRHPP
                     GLYGPENGRQALNLGAGVTAPTAAPMVAGARVEQLESAAPERELGPPLLAIAVLLLAI
                     DMLVALALRGLLRTRMAAIAVPLLLLAGIPRAHALETVANPALATRLGYILTGDAQVD
                     EISRQGLEGLSEYVDGRTAAILAEPDGVVPGKTHLSFYPLLYWPITPDAPALTPQQTA
                     ALNRYMSNGGIILIDTRDSGSGAGFAPGSDAALRRVAKGLVIPPLAPLTTADVLSRTF
                     YLLHDFPGRYTGATVWVQRDPERSNDSVSPVVIGGNDWAAAWAVDGTGRNPYAAIPGG
                     TEQRTMAYRFGVNLVMYALTGNYKGDQVHVPAILERLGQ"
     gene            complement(3931..4806)
                     /locus_tag="VFN42_07250"
     CDS             complement(3931..4806)
                     /locus_tag="VFN42_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET8996448.1"
                     /translation="MAIDPRRAEALASHLPPLLVAAERVASTVAQGVHGRRRVGQGDS
                     FWQFRRFVTGDPVGRIDWRQSAKSGRPAPHGWFVRETEWEAAQTICLWRDGSPSMRWH
                     SRSMAIEKRERADLLLLALAALLLRGGERVLMLRDGARAVWGRGGLDRLAEDLAGLPD
                     EPGGMPPALPLPRHASVVLFGDFLNPLEEIQSLVGRLAAAPTTGYLLQILDPAEALLP
                     YVGRIRFRGVERDGETLIPRVESIRDEYARRLKAQQDGIAAICSAAGFGFGIHRTDLP
                     PETALLALYTALGLK"
     gene            complement(4837..>5186)
                     /locus_tag="VFN42_07255"
     CDS             complement(4837..>5186)
                     /locus_tag="VFN42_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET8996449.1"
                     /translation="PEELMAAQALIRRVPVGEKVLDAILALVRSGRPETSTDDTVRRQ
                     VAWGPGPRAAQALMLASRARALLQGRLAPSVDDVVALAQPVLRHRMALNFSARADGVV
                     LNEVIDRLVGRIG"
BASE COUNT          858 a         1826 c         1784 g          718 t
ORIGIN      
        1 ggcgggcagg atgcttccaa gcggggtctc cgccaaaccg ccggagccgg agaactccgg
       61 tccgacattc atcagcagcg caccgccctc gcgcacccgt gtcgcgatgt tggccagata
      121 gggcagtggc agcagaccgc gattctggaa ctggtccagg atgatcaggt cgaactcgcc
      181 gatcttctcc tggaacagct cgcgcacggg aaaggcgatc agcgcgagct ggttgagcgg
      241 cgtcatgtcg tccttgtccg gcggacgcag gatggtgaag tgcaccaggt cgaccgacgg
      301 atcggatttc agcagccgcc gccaggtgcg caccgccggc gcgggcgcac cggaaatcag
      361 cagcacgcgc agccggtcgc gcacgccgtt gatctccacg acggcgcggt tgttcagggt
      421 ggacacctca cccggcagct tgtcggcgga catctcgatc acggtcgggc ccgcgcgggt
      481 gatcgggacg gtgatgttct cgggatggcc gaccggcacg ctctccgtca gcgccggctg
      541 gccgtcgcgg cggatggtca ggtccgccga actgttcgga tgcctcaccc cctggtcgtc
      601 gaccacgact ttcagcgtca ccgacttgcc gacgatgcca tagccgggcg cttcgatgac
      661 tcgcaggcgg cggtcgattt cctcgccctt cgccgggatc agcacattga gcggggcgcc
      721 gcctggcgtg ctcttcggaa gatcgctgat ctcgccgtca gtgatggcga tggtcccggc
      781 cagccgcgcc cgtggaatgt cgcccatggc gcggttgatg gcggcgaaga gctgggtgcc
      841 gttgtcaccc tgctccggga cattgatggt gcgcagctcc agtccgggca tcttgccggc
      901 ctgcgccgtt atcgccgccc gcgcctgctc ggccaattgc atgcggtcgc cgagcttcat
      961 cgaatcgctg tggtccatca ccagcaggcc gatgtcggac agaccctgcc aggtttcctg
     1021 caccaggcgc gggccggcga gccagcccag cagcaccgcg aaggcggcaa gacgcagcag
     1081 cgtgccgcgt gcgcggcgcc agacgccgat cgcgaccacc agcagggcga tgccgcccag
     1141 cagggcgatc acccagaccg ggaccagcgg atcgaggcgc agggcggaga tggcgtattc
     1201 gggatgcatc agccgccccc ccgaccacca cgatggcaag cgggcatcat tgccccagcc
     1261 gctcgagtat ggccggcacg tgcacctggt cgcccttgta gttcccggtc agcgcgtaca
     1321 tcaccaggtt caccccgaac cgataggcca tcgtacgctg ttccgtgccg cctggaattg
     1381 cggcgtaggg attgcggccg gtgccatcca ccgcccaggc cgcggcccag tcattgccac
     1441 cgatcaccac cgggctgacg ctgtcgttcg aacgctccgg atcgcgctgc acccacaccg
     1501 tggcgccggt atagcgcccg ggaaaatcat gcagcaggta gaaggtgcgc gacagcacgt
     1561 cggccgtggt cagcggcgcc agcggcggga tcaccagccc cttggcaacc cgccgcaagg
     1621 cggcgtcgct gcccggggcg aagccggcac cggaaccgga atcccgtgtg tcaatcagga
     1681 tgattccgcc attgctcata tagcggttca acgccgccgt ctgctgcggc gtcagcgccg
     1741 gcgcatccgg tgtgatcggc cagtacagca gcggatagaa gctcagatgc gtcttgccgg
     1801 ggaccacacc gtccggctcc gccaatatcg ccgcggtgcg gccatcgacg tattccgaca
     1861 gaccttccag gccctggcgg gagatttcgt ccacctgggc gtcaccggtg aggatgtagc
     1921 ccagccgggt cgccaacgcg ggattggcga ccgtttccag tgcatgggct cgcgggatgc
     1981 cggcaagcaa cagcagcggc acggcaatgg cggccatgcg cgtccgcagc aatccgcgca
     2041 gcgccagcgc gaccagcatg tcgattgcca gcagcagcac ggcgatggcc agcaacggcg
     2101 ggccaagttc gcgttccggc gccgccgatt ccagttgctc cacgcgggcg ccggcaacca
     2161 tgggcgccgc ggtgggtgcg gtgacccctg cccccaggtt caaagcctgc cgtccatttt
     2221 cggggccata caggccgggc gggtggcgtg gcgagatcat cgcctggcca aattcatccg
     2281 cccgcaggcc ggtcgccgat tgcggcgggg cacccagcat gccgaagccg tccagcgtct
     2341 cggccggaga cagcagcgtg tgcggcggtt cgctcttcac gccgacggac agcgacacca
     2401 gccggcgcag catgtcgacg aacagcccgg acaagggcag attcgaccag tccacgttgg
     2461 cggtgacatg gaacagcacg atgcggccgg cgcccttggt cgcttccgtc accagaggcg
     2521 tgccgtcctt cagcgccgcc caggtatgcg ccgccagatc ggcgctgggc tgtgccagaa
     2581 cctggcggtt gaccgtcaca tccgccggcg cgtccagccc ggcaaacggc gagttgggcg
     2641 ggaacggcgc caggccggcc ggcttgctcc aggacatggc gcccccgagc gcgcgatccc
     2701 cctgcagcag gggcaccggc atcagcgggt cggtctcgcc gatcggctgc tccgcggtgc
     2761 gagacccggc gaagcgaatc agcagcccgc cctgttcaac ccatttggcc acagcggcgc
     2821 gctccggtcc ggcgggtagc ggatcatccg ccaggatcag cacggacaat ttccgcttca
     2881 gcaatgtgtc gaggctgcct tcccgcaatt cggcataggg ctgcagcgcg cggctgacga
     2941 agtacagtga tcctacgaag ggcgtgttgg cggcgacggc gtcccccgcg gcgaggccca
     3001 ccggcctgcg gcggtagcgc tcgtccagca gaaacacgga gccggccgac tgcgagccct
     3061 gcagcaccag gcgcgccaac tcgttgcgga tttccggcgg cagcatcagc ggggcagagc
     3121 cgattgcagc accggccggc aagtcgaggg gcgtccgcgc cagagtgcga ccgtcgccgg
     3181 tctgggccag gaccacggcg gaggtcggcg ccggctgcgg aacttgcgcc aggcgcacca
     3241 ccaggcgatc ggcgttgcta tccggcggca acagcatgcg ggcggcggtg gcggcacaac
     3301 acagttcggt gacactgccg gcgtgccgca gggcctcgga aaagcggggg aagtcggacc
     3361 cgtcggtcag cccatcggcg agatacacca ccgccgcgcc ggcgtggtgc cactgatcca
     3421 gtgccgccgc tgcggcggcg cgatcggccg gccagggctc cggccgcaag gccgcgaggc
     3481 gtgaccgcag gctggcgatc ggcatgacag aggtgatgcg cggcaaggtg ccggtttccc
     3541 ccggggcggt ggtcagcaat gccgcctggc gcccggcacg cgccgcgcgg tccagcatgc
     3601 tgtccgccgc ctgcatgcgc ctggcccagt ccgaagcgga agcccatcca ttgtcgagca
     3661 ccagcagcat cggtccgctg ccggcaaatt cagtccccgc gttcagcacc ggccgggcca
     3721 gcgccacgat caccaacgcg gctgcgagca ggcgcaatgt catcagccac cagggcgtgc
     3781 gcgccggcgt ttcctccttg gcgtgcagac cgagcagcag gcggatggcg ggaaaactct
     3841 cgctgcgcgg cgcgggtggg gtgacgcgca gcaaccacca gagaaccggg aggccgatca
     3901 aggcgagcag cacccagggt gcggcaaaga tcatttgagg cccaaagcag tgtacaaggc
     3961 gagcaatgcg gtttccggtg gcaggtcggt gcggtggatg ccgaatccga acccggcggc
     4021 gctacaaatt gcggctatcc catcctgctg cgccttgagc cggcgagcgt actcatcccg
     4081 aatggactcg acacgaggga tcaaggtctc cccatcccgt tccacgccgc gaaagcggat
     4141 gcggcccaca tagggcagca gcgcctcggc cggatcaagg atctgcagca ggtagccggt
     4201 cgtgggcgca gcggcgagcc gtccgacgag ggactggatc tcctccagcg ggttcaggaa
     4261 atcgccgaac aggaccacgc tggcatggcg cgggagcggc agcgccggcg gcatgccacc
     4321 cggctcgtcc ggcaacccgg cgagatcctc cgcgaggcgg tccagcccgc cacgccccca
     4381 gacggcgcgg gcaccgtcgc gcagcatcag cacgcgctcc cccccgcgca gcagcagcgc
     4441 ggccagggcg agcaacagca ggtcggcccg ctcacgcttt tcaatcgcca tcgaccggga
     4501 atgccagcgc atggatggcg aaccgtcgcg ccacaggcag atggtctgcg cggcctccca
     4561 ttcggtttcg cgcacgaacc agccatgcgg ggcggggcgg ccggatttcg ccgactggcg
     4621 ccagtcgatg cgaccgactg ggtcgccggt gacgaagcgg cggaactgcc agaagctgtc
     4681 cccctgaccg acacggcgcc gcccgtgcac gccctgggcg acggtgctgg ccacgcgctc
     4741 cgccgcgacc agcagcggag gcaagtggga cgcgagtgcc tcggcgcggc gggggtctat
     4801 cgccatcggc acttcccgcc accacgggtc tggcgatcag ccaattctgc ccaccagccg
     4861 atcgatcacc tcattcagca cgacaccatc ggcccgggcg gagaaattca gcgccatgcg
     4921 gtgccgcagc accggctggg ccagcgccac cacgtcatcc acgctcggcg ccaggcggcc
     4981 ctgcagcaac gcgcgggcac ggctggccag catcagggcc tgcgcggccc gcggtcccgg
     5041 gccccaggca acctggcggc gcaccgtgtc atcggtcgag gtttccggcc ggccgctgcg
     5101 caccagggcg aggatcgcgt ccagcacctt ctcacccacc ggcacacggc ggatcagagc
     5161 ctgggcggcc atcagctcct ccggcg
//
LOCUS       DASSZH010000336         5378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_996802, whole genome shotgun sequence.
ACCESSION   DASSZH010000336 DASSZH010000000
VERSION     DASSZH010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996802"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..424)
                     /locus_tag="VFN42_07260"
     CDS             complement(50..424)
                     /locus_tag="VFN42_07260"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET8996450.1"
                     /translation="MTDAVLLLARLLMSVVFIRSGIGKLLAPAGTMATFHHDRLPLVG
                     AVYALAVAVEIGGGVAVLLGWRTRAAAIILAAWCIATAAVGHWHPGISAQMVQFTKNL
                     CMAGGFLLLWAQGAGRFSIDRR"
     gene            complement(434..856)
                     /gene="ndk"
                     /locus_tag="VFN42_07265"
     CDS             complement(434..856)
                     /gene="ndk"
                     /locus_tag="VFN42_07265"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET8996451.1"
                     /translation="MATERTLSIIKPDATRRNLTGKVNACLEAAGLRIVAQKRVRLTT
                     QQAEAFYAVHKERPFFRGLVEFMTSGPVVLQVLEGDNAVAKNREVMGATDPAKAAAGT
                     IRAQFAENIEANSVHGSDSAENAANEIAFFFAGIEIAG"
     gene            complement(988..2613)
                     /locus_tag="VFN42_07270"
     CDS             complement(988..2613)
                     /locus_tag="VFN42_07270"
                     /inference="COORDINATES: protein motif:HMM:NF014683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HET8996452.1"
                     /translation="MFTQILDPVGNLGLTCLVALVPVVLLLVLLAVFRMSAWLAVLIG
                     SIVTFVLAVAVWRMPLDTGFRAYIYGSATGVWNVDWITFWGLVLFNTLSISGMFENFR
                     RWLVSQGTLDIRVQTMLFAWAFGALLEGLVGFGYPWAVVAPILITLGLSDLDAIRVAA
                     IANNAPVSYGALGAPIIALAAVTGYPLLALSGSVGSIVAVLALLPPWVLIYLVAGKRG
                     FRDGWPLAIVGSLGYIAGQWPVAYYFGPYLPDITGSIVCFCSLLLLLKFWQPAQILGH
                     GGVPVSASEKAAAAADHGLSTSEVLLAWMPFAVLVVVVAAWTGPWSHLPGVSWFFASV
                     KAVAPETKTTIVAAFNFAPWVGGSAILAAWLIISVLLAGFGKLKLAEVGAIFAKTFDQ
                     MWGALLVGVFIFGLAFVFNYSGMASSLAKGFSYLGPWFIVVAPLLGFIGVALSGSNTS
                     TNAMFGKFQALVGVQLGFPPLLLPTLNSVGSEIGKPIAPQTASVGVSTSRFVRNEGEV
                     IRHNFGWTLVLLVYLIVIGVGFYLLRPGMLMLH"
     gene            complement(2881..4341)
                     /locus_tag="VFN42_07275"
     CDS             complement(2881..4341)
                     /locus_tag="VFN42_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006558981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8996453.1"
                     /translation="MSESATEPASPEPMRPSARMVSLIVASAMFMEQLDGTVLATALP
                     TMAKSFHVAPLRMNVALTAYLLTLAMFIPASGRIADRFGSRTVFRAAIGLFTLGSVAC
                     AQAPSLAFLVGARMLQGAGGAMMSPVGRLVLLRAVSKSELVRAMAWLMIPATIGPIVG
                     PPVGGFLVTYLSWHWIFYINVPIGLAGILLVTRYIEEMREETRTKFDLRGLILAGSAL
                     SCLMFGIEMASRGVGSRLVTLGLVLGGAASAGLYALHARRHPRPMLDFRLMRIATFRM
                     SVLCGTLSRIAVGAMPFLLPMMLQLGFHMSAARSGAITFISSVGSLAMRLAAPWFLRR
                     LGFRTVLIWIGLLATLLLACTAAFRPSWPIEAIYALLLANGFFQSLQFMAYNTIAYAD
                     VPRPEMSAATSFYTTFQQMSLTLGIAISAAVLAGSMAMAHHTHAMLSDFSVAFLFVSS
                     ISLFAPLLATRLDPGAGAELSGHRDRPGKPRLAAAE"
     gene            complement(4426..4953)
                     /locus_tag="VFN42_07280"
     CDS             complement(4426..4953)
                     /locus_tag="VFN42_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996454.1"
                     /translation="MSDVPPTGPGFGELDEVIGRFDSSDRMDDAIGRLELAGFARADL
                     SVPEPSPPVERATPESGAEAPYTGDDAQTARTLHTSGVASVAALAAAGITIATGGAAA
                     PAFAAAIGAGALAGGATFVVSEGADAQEQEDRDAKAAAGILLISVRAPDAAKQAEASA
                     ILRDAGALEVQVRAH"
BASE COUNT          950 a         1798 c         1896 g          734 t
ORIGIN      
        1 acaggcttgg cagcatggtc tcctcctcag ccggcctgtg gcgccggcgt catctccggt
       61 cgatgctgaa ccgtccggcc ccctgtgccc agagcagcag gaagccaccg gccatgcaca
      121 ggtttttggt gaactgtacc atctgggcgc tgatgccggg atgccaatgg cccaccgccg
      181 cggtggcgat gcaccaggcg gccaggatga tggcggcggc acgggtgcgc cagccgagca
      241 gcacggcgac gccgccgccg atctccaccg ccacggccag ggcatagacg gcgccgacca
      301 gcggcaggcg gtcgtggtga aacgtcgcca tggttccggc cggcgccagc agcttgccga
      361 tcccgctgcg gatgaacacg acgctcatca gcaggcgggc cagcagcagg acggcgtccg
      421 tcatgacgca ggatcagccg gcgatttcga taccggcgaa gaagaaggcg atctcgttgg
      481 cggcgttctc ggcgctgtcg gagccgtgca ccgagttggc ttcgatgttc tcggcgaact
      541 gggcgcggat ggtgccggcg gcggccttgg ccgggtcggt ggcacccatc acctcacggt
      601 tcttcgccac cgcgttgtcg ccttccagca cctggagcac gaccgggccg gaggtcatga
      661 attcgaccag gccgcggaag aaggggcgtt ccttgtgcac ggcgtagaag gcttcggcct
      721 gctgcgtggt caggcggacg cgcttctggg cgacgatgcg caggccggct gcctcgaggc
      781 aggcgttgac cttgccggtg aggttccgcc gcgtggcgtc gggcttgatg atggacagcg
      841 tgcgctcggt ggccatgtct ggcgggctcc tggatcgaag gtcggaaatt cggccgggtg
      901 gttaggcggt ccagaggccg ggggcaaggg cggtgctggc ggtgggggct cggccgtgcc
      961 gccgcgcccc cacgcgccgg ctggcggtca gtgcagcatc agcatgccgg ggcgcagcag
     1021 gtagaagccg accccgatga cgatcaggta gaccaacagc acgagagtcc agccgaagtt
     1081 gtgccggatc acctcgcctt cgttacgcac gaaccggctg gtggacacgc cgacgctggc
     1141 ggtctgcggg gcgatcggct tgccgatctc cgatcccacc gagttcagcg tgggcagcag
     1201 cagcggcggg aagccaagct gcacgccaac cagtgcctgg aacttgccga acatcgcgtt
     1261 ggtggaggtg ttggagccgg acagcgccac gccgatgaag cccagcaacg gcgccacgac
     1321 gatgaaccac gggccgagat aggagaagcc cttggccagc gacgaagcca tgccggagta
     1381 gttgaacacg aaggccaggc cgaagatgaa cacgccgacc agcagcgcgc cccacatctg
     1441 gtcgaaggtc ttggcgaaga tggcgccgac ctcggcgagt ttcagcttgc cgaacccggc
     1501 gagcagcacg ctgatgatca gccaggccgc caggatggcg ctgccgccga cccagggggc
     1561 gaagttgaaa gccgccacga tggtggtctt ggtttccggt gcaaccgcct tgacgctggc
     1621 gaagaaccag ctcacgcccg gcagatgcga ccacggtccg gtccaggccg cgaccacaac
     1681 caccagcacg gcgaagggca tccaggcgag cagcacctcg ctggtgctca gcccatggtc
     1741 ggctgccgcc gcagcttttt cgctggcgct gaccggcacg ccgccatgcc ccaggatctg
     1801 cgccggctgc cagaacttca gcagcagcag cagcgagcag aagcagacga tggaaccggt
     1861 gatgtcgggc aaataggggc cgaagtaata cgccaccggc cactggccgg cgatgtagcc
     1921 gagcgaaccg acgatcgcca gcggccagcc gtcgcggaaa ccgcgcttgc cggcgacgag
     1981 gtagatcagc acccagggcg gcagcagcgc cagcacggcg acgatgctgc cgaccgagcc
     2041 ggacagcgcc agcagcggat agccggtgac cgcggccagg gcgatgatcg gcgcgcccag
     2101 cgcgccgtac gacaccgggg cgttgttggc gatggcggcg acgcggatcg cgtccaggtc
     2161 gctcaggccg agggtgatca ggatcggtgc gacgacggcc caggggtagc cgaacccgac
     2221 caggccttcc agcagcgcgc cgaaggccca ggcgaacagc atggtctgca cgcggatatc
     2281 cagcgtgccc tgcgacacca gccagcgccg gaaattctcg aacatgccgc tgatcgacag
     2341 cgtgttgaac agcaccagac cccagaaggt gatccagtca acattccata cgccggtggc
     2401 ggagccgtag atgtaggcgc ggaagccggt gtcgagcggc atgcgccaga cggcgacggc
     2461 cagcacgaaa gtaacgatcg agccgatcag cactgccagc caggccgaca tgcggaacac
     2521 cgcaagcagc accagcagca gcacgaccgg caccagggcc accaagcagg tgagtccgag
     2581 gttgccgacc ggatccagga tctgggtaaa catcaggtct ccctatccgc ccgtgttggg
     2641 cggggatgcg gcttcggccc tggcaggccg gcttcgggcc gaaatccggg tattgtgcct
     2701 tccggtgagg taacgttatc agtcgcgcgt cggccccgcg ccgtggaacc gccgcgcttg
     2761 cgggacgtgg acctgccggg gcgatgccgc atttttgccg aaccggcctt gatttggatc
     2821 aatcaccagc ggcaacgcgg atgtatttca ccggccgtcc gcttggccga gcgcgaacgc
     2881 ctactccgcg gcggcaagcc tgggctttcc cgggcgatcg cgatggccgc tcagctcggc
     2941 gccggccccc gggtccagcc gtgtcgccag cagtggggcg aacagcgaga tgctggagac
     3001 gaacagaaac gccaccgaga aatccgacag catggcgtgc gtgtggtgcg ccatcgccat
     3061 cgacccggcc agcaccgcgg cggagattgc gatgcccagg gtcagcgaca tctgctggaa
     3121 cgtggtgtag aagctggtcg ccgcgctcat ctccggccgc ggtacgtcgg cataggcgat
     3181 cgtgttgtac gccatgaact gcagcgactg gaaaaacccg ttggcgagca gcagggcgta
     3241 gatcgcctcg atcggccagg acgggcggaa cgccgcggtg caggccagca acagcgtcgc
     3301 cagcaggccg atccagatca gcacggtgcg gaagcccagc cggcgcagga accacggcgc
     3361 cgccagccgc atcgccagcg agcccaccga actgatgaag gtgatggcgc cgctgcgtgc
     3421 ggccgacatg tggaagccga gctgcagcat catcggcagc aggaacggca tcgcaccgac
     3481 ggcgatgcgc gacagcgtgc cgcacagcac cgacatgcgg aacgtggcaa tgcgcatcag
     3541 ccggaaatcc agcatcggtc gcggatgccg ccgcgcgtgc agcgcgtaca gcccggccga
     3601 cgccgcaccg cccagcacca ggcccagcgt gaccaggcgg gagccgacgc cgcggctcgc
     3661 catctcgatg ccgaacatca ggcaggacag cgcgctgccg gcgaggatca gcccgcgcag
     3721 atcgaacttg gtgcgggtct cctcacgcat ctcctcgatg tagcgcgtca ccagcaggat
     3781 gccggccagg ccgatcggca cgttgatgta gaaaatccaa tgccaggaca gataggtgac
     3841 caggaagccg cccaccggcg ggccgacgat cgggccgatc gtggcgggga tcatcagcca
     3901 ggccatggcg cggaccagtt ccgatttgga aaccgcacgc agcagcacca gccgcccgac
     3961 cggcgacatc atggccccgc cggcgccctg cagcatgcgg gcgcccacca ggaaggccag
     4021 gctgggggcc tgggcgcagg cgaccgagcc gagggtgaac aggccgatgg cggcgcggaa
     4081 cacggtgcgg gagccgaacc ggtcggcgat ccgcccgctg gccgggatga acatcgccag
     4141 ggtcagcaga taggcggtca gcgccacgtt catgcgtagc ggcgcgacat ggaagctctt
     4201 ggccatggtc ggcagcgcgg tggcgagcac cgtgccgtcg agctgttcca tgaacatcgc
     4261 gctggcgacg atcagcgaca ccatgcgcgc gctggggcgc atgggctcgg gtgaggcggg
     4321 ctcggtggct gactcggaca ttgcgccgca gcatagggcc ggccatgccc ggcggtagcg
     4381 ccgccgctgc atggccgggt ggagaaacgc gcgatgccgg ccgggtcagt gcgcccggac
     4441 ctgcacctcc agcgcgccgg catcccgcag gatggcgctc gcctcggcct gctttgccgc
     4501 atccggggcg cgcaccgaga tcagcaggat cccggccgcg gccttggcgt cgcgatcctc
     4561 ctgttcctgc gcgtcggcgc cctcgctgac gacaaacgtg gcgccgccgg ccagggcgcc
     4621 cgcgccgatc gccgccgcaa aggccggcgc ggcggccccg ccggtcgcga tggtgatgcc
     4681 tgccgccgcc agggcggcca cgctggccac gccgctggtg tgcagcgtgc gtgcggtttg
     4741 cgcatcgtcg ccggtatagg gcgcctcggc gcccgattcg ggcgtggccc gctcgaccgg
     4801 cggcgagggc tcggggacgc tgagatcggc gcgcgcgaac ccggcgagtt ccaggcgccc
     4861 gatcgcgtca tccatcctgt cggagctgtc gaagcggccg atcacctcat ccagttcgcc
     4921 aaagccgggg ccggttgggg gaacgtcgga catcagtgta cctcgcaaat gcctgttgac
     4981 cgaacgcagc ggcgatgccg tcggttcgct ccacagacaa tccggggttt gctgcggttc
     5041 cccgcgatcg cgactcatgc aagcgggaag gggaagccag ccagcagccc gcaccatgtc
     5101 ttggcgggcg gaaccccgtt cttcccgtct tggccggact tgacccggcc atctaccccc
     5161 gacaaggcgt gttccgttgc aaccgacccg agcgttcccg ggcggatggc cgggtcaagc
     5221 ccggccacga cgcggaaatc gcgctctgcg cggggcttat gttagcgccc atgggatcaa
     5281 gtccggccat gacacgagag ttcgcggttg ccgtacctca gccagcgcga atggccatgc
     5341 tccggcagcg cttaaacctt ggcgggcttt ccggcacc
//
LOCUS       DASSZH010000337         3711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_110209, whole genome shotgun sequence.
ACCESSION   DASSZH010000337 DASSZH010000000
VERSION     DASSZH010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110209"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..915
                     /locus_tag="VFN42_07285"
     CDS             208..915
                     /locus_tag="VFN42_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HET8996455.1"
                     /translation="MIDFYYWPTPNGWKVSIMLEETALPYRMIPVNIGKGDQFKPDFL
                     AISPNNRMPAIVDHDDPDGPLPIFESGPILLHLAEKSGRFAPSGRVARKEMMEWLFWQ
                     VGNLGPMAGQLSHFVNYAQGEHSYSHQRYANEYNRCLGVLERRLTDRAYILGDSYSIA
                     DIIAWPWVLIAKPLGQDLAEFPNVSRWRDAIKQRPAVQRGVDLGKELRRRAPPGEEER
                     KILFNQTASSIGKGSAA"
     gene            912..1751
                     /locus_tag="VFN42_07290"
     CDS             912..1751
                     /locus_tag="VFN42_07290"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8996456.1"
                     /translation="MSIDLKGKTAVVTGAAGGIGLGIAKALAGAGANLVLADLQPDKL
                     EPARAAIEALGVRAVAVPVDVSDADSVANVGRVAEKNFGKLHVAVNNAGVAMHGTPIE
                     QVTPQEWQWLIGVNVMGVINGIRAFMPLIRKHGEGGHVVNTASVSGFFVREGRNQGAY
                     AMSKYAVLALSEALEQETAGSGIGVSVLCPGPVTTNIFRSAATRPERFGGAYQRPSQE
                     AMGGMLGTGLPPEVVGQRVLQGMRDGAFYILTHASERDVVKARHARIEAAFDNAAAWE
                     TSR"
     gene            1769..2983
                     /gene="acdA"
                     /locus_tag="VFN42_07295"
     CDS             1769..2983
                     /gene="acdA"
                     /locus_tag="VFN42_07295"
                     /EC_number="3.13.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-sulfinopropanoyl-CoA desulfinase"
                     /protein_id="HET8996457.1"
                     /translation="MTFALSPAQLQLREQARHIAETIIAPRAAEVDRSEQYPWDNVAA
                     LRDAGLMGYTIPQEYGGSGGSFLDAAIIIEEMARVCGVTGRIAVEANMGAISAVMRYG
                     TEAQKRLAAELVLAGDKPAICITEPDAGSAATEMTTRADRHQGGYVLSGKKHWITGAG
                     VSRLHLIFARIHDERGRPQGIGGFLVVRDADAGTPKGLVIGKREPTMGLRGIPEAEVI
                     CDNLEVPDDMVLCTESGAPVGFSALMNAYNSQRVGAATVALGLAQGAFDLALDWVKQR
                     HQFGRPIAEFQGLQWMLADMAIQLNAARLGIHQAAASADPFPDPLLAAQAKVFASEMA
                     IKVTNDALQMFGARGYSRDRPLERMARDARMFTIGGGTAQVLRTLIAGRVLGWKVPQT
                     RDAAAGPARQAG"
     gene            3005..>3711
                     /locus_tag="VFN42_07300"
     CDS             3005..>3711
                     /locus_tag="VFN42_07300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8996458.1"
                     /translation="MAQATDVVVIGGGAIGSSIAYFLRQRTAPPRVTVVERDPSYALA
                     STPRASGGVRQLFSGRENIALSNFSIPFFETFAQAMAVGGTLAEIGFRKGGYLFIVDD
                     RGAAMLEESAAVQRDMGVRVDLLDRLGLKDRFPSMYVDDLTLAAHSLDDGWLDPHSVL
                     QGFQRKAVELGANYLADAVTGIELDGGLVRRVRLASGQALNADYVVNAAGAWADQVCA
                     MIGMRSPIRPMRRFEHY"
BASE COUNT          607 a         1264 c         1267 g          573 t
ORIGIN      
        1 gcgatcgaac aactcgaggg tgcggattat gacggcgttg cgccgcttcc gtggcccgtg
       61 aactgccctt tcacgctcga ccaactgctt cagggtcggc gtgccgagct ggaacagcag
      121 ttgaagggcg atagccagcc gcacggttga ccctcggcag cccggttgcc atcgtcccgc
      181 ccccagcccg cgcgggagca acgccgcatg atcgacttct actactggcc cacgccgaac
      241 ggctggaagg tctccatcat gctggaggag accgccctgc cctaccgcat gatcccggtg
      301 aacatcggca agggtgacca gttcaagccg gacttcctgg cgataagccc gaataaccgc
      361 atgccggcga tcgtcgatca cgacgacccg gatggtccgc tgccgatctt cgagtccggc
      421 ccgatcctgc tgcacctggc ggagaagtcc ggccgcttcg cgccctccgg ccgcgtcgcc
      481 cgcaaggaga tgatggagtg gctgttctgg caggtgggca atctggggcc catggccggc
      541 cagctcagcc atttcgtcaa ctatgcgcag ggcgagcaca gctattccca ccagcgctat
      601 gccaacgagt acaaccgctg cctgggcgtg ctggaacggc gcctcacgga ccgcgcctac
      661 atcctgggcg actcctacag tatcgccgac atcatcgcct ggccctgggt gctgatcgcc
      721 aagccgctcg gccaggatct ggcggaattc cccaacgtgt cccgctggcg tgacgccatc
      781 aagcagcgcc ctgcggtgca gcgtggcgtc gatctgggca aggagctgcg ccgccgcgcg
      841 ccgcccggcg aggaggaacg caagatcctg ttcaaccaga cggcatcctc catcggcaaa
      901 ggcagcgcgg catgagcatc gatctgaagg gcaagaccgc cgtggtgacc ggcgccgccg
      961 gcggcatcgg cctgggcatc gccaaggcgc tggccggcgc cggcgccaac ctggtgctgg
     1021 ccgatttgca gccggacaag ctggagccgg cccgcgccgc catcgaggcg ctgggcgtgc
     1081 gcgccgtcgc cgtgccggtg gatgtgtccg acgccgattc ggtcgccaat gtcggccgcg
     1141 tcgcggagaa gaatttcgga aaactgcatg tcgcggtgaa caatgccggc gtggcgatgc
     1201 acggcacgcc gatcgagcag gtgacgccgc aggaatggca gtggctgatc ggcgtcaacg
     1261 tgatgggcgt gatcaacggc atccgcgcgt tcatgccgct gatccgcaag catggcgaag
     1321 gcgggcacgt ggtgaacacc gcgtcggtgt cgggcttctt cgtgcgtgag ggccgcaacc
     1381 agggcgccta tgcgatgagc aaatacgccg tgctcgcgct gtcggaagcg ctggagcagg
     1441 agacggcagg cagcggcatc ggcgtgtccg tgctgtgccc cggcccggtg accaccaaca
     1501 tcttccgctc cgccgccacc cgcccggaac gcttcggcgg cgcctaccag cgtccgtcgc
     1561 aggaggctat gggcggcatg ctgggcacgg gtctgccgcc ggaggtggtc ggccagcgcg
     1621 tgctgcaggg catgcgcgac ggcgcgttct acatcctgac gcatgccagc gagcgcgatg
     1681 tggtgaaagc ccgccacgcc cgcatcgagg cggcgttcga caacgccgcc gcctgggaaa
     1741 ccagccgctg acatccgtgg agcccgccat gaccttcgcc ctcagccccg cccagcttca
     1801 gctccgcgag caggcccgcc acatcgcgga aaccatcatc gccccgcgcg ccgccgaagt
     1861 ggaccgcagc gagcaatacc cctgggacaa tgtcgccgcg ctgcgcgacg ccgggctgat
     1921 gggctacacc atcccgcagg aatacggcgg ctccggcggc agcttcctcg acgccgcgat
     1981 catcatcgag gaaatggcgc gcgtctgcgg cgtcaccggg cgcatcgcgg tagaagccaa
     2041 tatgggcgcc atctccgcgg tgatgcgcta cggcaccgaa gcgcagaagc gcctcgccgc
     2101 cgagctggtg ctggccggcg acaagccggc gatctgcatc accgagccgg acgccggctc
     2161 cgccgccacc gagatgacca cccgcgccga ccgccaccag ggcggctacg tgctctccgg
     2221 caagaagcac tggatcaccg gcgccggcgt gtcccgcctg catttgatct tcgcgcgtat
     2281 ccacgacgag cgcggcaggc cgcagggcat cggcggtttc ctcgtggtgc gcgatgccga
     2341 cgccggcacc ccgaagggcc tggtcatcgg caagcgcgaa cccaccatgg gcctgcgcgg
     2401 catcccggaa gccgaggtga tctgcgacaa cctggaagtc cccgacgaca tggtgctgtg
     2461 cacggaatcc ggcgcgccgg tcggattttc cgcgctgatg aacgcctata acagtcagcg
     2521 cgtcggagcg gccaccgtgg cgctcggcct cgcgcagggc gcgttcgacc tggcgctgga
     2581 ctgggtgaaa cagcgccacc agttcggccg cccgatcgcg gaattccagg gcctgcaatg
     2641 gatgctcgcc gacatggcga tccagttgaa cgcggcgcgg ctgggcatcc accaggcggc
     2701 agcgagcgcc gatcccttcc ccgatccgct gctggcggca caggcgaaag tgttcgcctc
     2761 ggaaatggcg atcaaggtca ccaacgacgc gctgcagatg ttcggcgccc gcggctattc
     2821 ccgcgatcgc ccgctggagc gcatggcgcg cgacgcccgc atgttcacca tcggcggcgg
     2881 caccgcgcag gtgctgcgca cgttgatcgc cggccgggtg ctcggctgga aagtgccgca
     2941 gacccgcgac gcggcggctg ggccggcgcg gcaggcgggc tgagctacag ggcaggtggc
     3001 cggaatggcc caggcaaccg atgtcgtcgt gattggcggt ggcgccatcg gatcgagcat
     3061 cgcgtatttc ctacgccaac gcaccgcgcc cccgcgggtg acggtggtgg aacgcgatcc
     3121 gagctacgcg cttgcgtcca cgccgcgcgc ctccggcgga gtgcgacagc tgttttcggg
     3181 acgtgagaac attgccctgt cgaacttcag tattccgttc ttcgagacat tcgcgcaggc
     3241 catggcggtg ggcggcacgc tggcggagat cggcttccgc aagggcggct atctgttcat
     3301 cgtcgatgat cgtggcgcgg cgatgctgga ggaaagcgcc gccgtgcagc gcgacatggg
     3361 tgtgcgggtc gacctgctgg accggctcgg cctgaaggat cgcttcccgt caatgtatgt
     3421 ggatgatctt accctggccg cccattcgct ggacgatggg tggctcgatc cgcacagcgt
     3481 gcttcagggt ttccagcgca aggcggtcga actgggcgcc aactacctcg ccgatgcggt
     3541 gactggaatc gagttggacg gtggcctggt ccgccgggtg cggcttgcct ccggccaggc
     3601 tctcaacgcc gattacgtgg tgaacgccgc gggtgcctgg gccgaccagg tctgcgcgat
     3661 gatcggcatg cgcagcccga tccggccgat gcgccgcttc gagcactact t
//
LOCUS       DASSZH010000338         2545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1678663, whole genome shotgun sequence.
ACCESSION   DASSZH010000338 DASSZH010000000
VERSION     DASSZH010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678663"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..614)
                     /locus_tag="VFN42_07305"
     CDS             complement(141..614)
                     /locus_tag="VFN42_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET8996459.1"
                     /translation="MSTTEAVSFETTRHVRATCLCLHLQQAARAIARRFDDCLRPTGL
                     TNGQFSLLMALNRPEPPTMSSVATLLAMDRTTLTASVKPLERRNLLRVVVDPVDRRSR
                     RLILTRAGKAALNSAYPLWQREHARLDGLVADPQRVRDDLQALSGTASTCDGPNL"
     gene            662..931
                     /locus_tag="VFN42_07310"
     CDS             662..931
                     /locus_tag="VFN42_07310"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET8996460.1"
                     /translation="MPVVSFTSALQRFMPVADAVVDASTVGEALAAVFAVQPALRGYL
                     LDDQGVLRRHVAIYVGGDCVRDRQRLSDPVGPQDEIHVFQALSGG"
     gene            942..2030
                     /locus_tag="VFN42_07315"
     CDS             942..2030
                     /locus_tag="VFN42_07315"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HET8996461.1"
                     /translation="MAERLYVGTRKGLFELARGRDGWDIAAVHFLGDPVSAVLATADG
                     AVLAGLDLGHFGAKLWRRDSGGGWREVPAPAFPPKPEDVEDDPHPWSLGKIWAIEPGG
                     VPGRLWIGTMPGGLFRSDDAGETWSLNEPLWRMPQRRKWMGVAGGEQPGICAVLVDRR
                     DPAVLRLGISTAGVWETTDTGATWQLINQGMYNEYMPPEQSGDPIAQDIHMLAHCAAQ
                     PDVVWCQHHNGVFRSSDAGHNWQEVTAIRPSKFGFAVVAHPRDPNTAWFVPAAKDERR
                     IPLDAKVVVARTRDGGESFVVLANGLPQRHAYDLVWRHALAVDGSGEHLAMGSTSGGL
                     WISENGGESWSSPEIRLPPVAVVRFAAA"
     gene            complement(2174..>2545)
                     /locus_tag="VFN42_07320"
     CDS             complement(2174..>2545)
                     /locus_tag="VFN42_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008854077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8996462.1"
                     /translation="HVTVGSNDIGRSKKFYDAVFGAMGGKPGSQDAKGRLIYVHNGGL
                     FMVTKPIDGKAATHANGGTIGFKMASQEQADAWHKAGVENGGTAIEDPPGVRQGSAGP
                     MYLAYLRDPDGNKLCALHRMG"
BASE COUNT          375 a          802 c          930 g          438 t
ORIGIN      
        1 cggaagatcg ccgtgctgcg caagcacatg cctttgccgc tgggcttggc cacgcgggcc
       61 tggaacaagc tgatgcgcaa tgcggcggcc gtgtcactgc gtcccgccgc ctcggcgcgg
      121 gcgggcgacc tgctgtcgtc ctagaggttt ggcccgtcgc aggtcgatgc ggtccctgaa
      181 agcgcctgca ggtcatcccg cacacgctgc gggtccgcca ccaacccgtc gagccgtgcg
      241 tgttcccgct gccagagcgg ataggcgctg ttgagcgcgg ccttcccggc acgcgtgaga
      301 atcaggcggc ggctgcgccg atccaccgga tcaaccacga cgcgcagcag gttgcggcgc
      361 tccagcggct tcacgcttgc ggtgagcgtg gtgcggtcca tggcgagcag cgtggcgacg
      421 ctgctcatgg ttggtggctc cgggcggttc agcgccatca gcagcgagaa ttgtccgttc
      481 gtcaggccgg tcggccgcag gcaatcgtcg aaccggcgcg cgatggcgcg ggccgcctgt
      541 tgcagatgca ggcaaaggca ggtggcgcgg acatgccgcg tggtctcgaa ggaaaccgct
      601 tcggtggttg acatgccatt tcatatgttg atatcaacct aaacgcaaga tgggaggttc
      661 catgccggtg gtctcgttca ccagcgcgct ccagcgcttc atgccggtgg cggacgcggt
      721 ggtggatgcg agtacggtcg gcgaggcgct ggccgcggtg ttcgccgtac agccggcgtt
      781 gcgcggctac ttgctggacg accagggcgt gctgcgccgg catgtcgcca tttatgtcgg
      841 cggcgactgc gtgcgcgacc gccagcggct gagcgatccg gtcggtccgc aagacgaaat
      901 ccatgtgttc caggcgctgt cgggcgggtg aggggaaagc aatggcggaa cgtctgtatg
      961 tcggcacccg caagggcctg ttcgaactgg cgcgtgggcg cgacggctgg gacatcgcag
     1021 cggtgcattt cctcggggat ccggtgtcgg cggtgctggc gaccgcggac ggcgcggtgc
     1081 tggccgggct cgacctgggg catttcggcg cgaagctgtg gcgccgcgat tccggcggcg
     1141 gctggcggga agtgccggcg ccggcgtttc cgcccaagcc cgaggatgtg gaggatgacc
     1201 cgcatccctg gtccctgggc aagatctggg cgatcgaacc cggcggcgtg ccgggccggc
     1261 tgtggatcgg caccatgccg ggcgggctgt tccggtccga cgatgcaggg gaaacctggt
     1321 cactgaacga accgctctgg cgcatgccgc agcgccggaa atggatgggc gtggccggcg
     1381 gcgagcagcc cggcatctgc gcggtgctgg tggatcgccg cgatccggcg gtgttgcggc
     1441 tgggcatttc cacggccggc gtctgggaga ccaccgatac cggcgccacg tggcaactga
     1501 tcaaccaggg catgtacaac gaatacatgc cgccggagca atccggcgat ccgatcgcgc
     1561 aggacattca catgctggcg cactgcgcgg cgcagccgga cgtggtgtgg tgccagcacc
     1621 ataacggcgt gttccgttcc tccgacgccg gccataactg gcaggaggtc accgcgatcc
     1681 gcccgtccaa attcggcttc gcggtggtgg cgcatccgcg cgacccgaat accgcgtggt
     1741 tcgtgccagc ggcgaaggac gagcggcgga ttccgttgga cgccaaggtg gtggtggcgc
     1801 gcacccgcga tggaggggaa agcttcgtgg tgctcgcgaa cgggctgccg cagcggcacg
     1861 cctatgacct ggtgtggcgt catgcgctgg cggtggatgg cagcggggaa cacctggcga
     1921 tgggttccac cagcggcgga ttatggattt ccgagaacgg cggcgaatcc tggtcgtcgc
     1981 cggagattcg gctgccgccg gtcgcggtgg tgcggttcgc cgctgcttga ttcgccagac
     2041 gcggtttcgc gcgcacgagc ggttgccgca caccgggccc aggccatgac acaatgccag
     2101 gaacgggcac gccaggcacc cgtatcggcg ggtgcactgg cggtggtcac tgtatcccgt
     2161 tagcgcggga cgctcagccc atgcggtgga gggcgcacag cttgttgccg tcgggatcgc
     2221 gcaaataggc aaggtacatc ggcccggcgc tgccctggcg aacacccggc gggtcctcga
     2281 tcgccgtgcc gccgttttcg acgcccgcct tgtgccaggc atcggcttgt tcctggctgg
     2341 ccatcttgaa gccgatcgtg ccgccgttgg cgtgcgtcgc cgccttgccg tcgatcggct
     2401 ttgtcaccat gaacaggccg ccattgtgca cgtagatcag gcggcccttg gcgtcctgcg
     2461 agccgggctt gccgcccatg gcaccgaata ccgcatcgta gaacttcttc gaccggccga
     2521 tatcgttgct gccgaccgtc acgtg
//
LOCUS       DASSZH010000339         8211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_416783, whole genome shotgun sequence.
ACCESSION   DASSZH010000339 DASSZH010000000
VERSION     DASSZH010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8211
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416783"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="VFN42_07325"
     CDS             complement(<1..848)
                     /locus_tag="VFN42_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8996463.1"
                     /translation="MFPSQPVEAKRRCTAMIESFRQRACVCDVSKGNTAIAAPFHSLQ
                     TRRAVMSDTPHPPQHQQSQPGHENQMEPRPQSAMARYRPAGKLSGKAALISGGDSGIG
                     RAVAVAFAKEGADVVIAYLDEHDDAKETVRLIEAEGRRAIAVPGDIGNSDFCREVARR
                     AVQEFGRIDVLVNNAAEQHMQEKLEDISDEQLERTFRTNIFGQFFLSRAVLPHMPDGG
                     AIVNTTSVTAYKGSPTLLDYASTKGAIVAFTRSLAIQLMQRRIRVNAVAPGPIWTPLI
                     PATFPA"
     gene            949..2034
                     /locus_tag="VFN42_07330"
     CDS             949..2034
                     /locus_tag="VFN42_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET8996464.1"
                     /translation="MLITGGAGFIGCHVCDALLARGYRVRVLDRMLEQVHSNRPPNPI
                     LRETELLVGDVRDAALVGKALRGVDAVIHLAAEVGVGQSMYEIERYVGGNDQGTAVLL
                     QALIAQPVRRVVVASSMSIYGEGLYRTAEGGLVDDAVRGTTAWDPVDAQGRKLVPVAT
                     PESKRASLASVYAITKFVQERLVLNVAPAYDMQAVALRLFNVFGPGQALSNPYTGVLA
                     IFAAALLNGRAPAVFEDGEQRRDFVHVEDVARAFVLALEHPAAPGGVFNIGSGESVSI
                     REVGEMIADAMGRGDLPPTILGKARTGDIRHCFADITQARQVLGFEPRRFLRSSLGEL
                     ANWVRRQQAIDRAAEAQRELETRGLVA"
     gene            2031..3095
                     /locus_tag="VFN42_07335"
     CDS             2031..3095
                     /locus_tag="VFN42_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET8996465.1"
                     /translation="MSGTFSVVGSRPPVLITGGAGFIGCNLADRLASDGENVLVYDSL
                     ARPGVEANLAWLRARHPRRISSTVADVRDADAVGDAARQSAAVFHLAAQVAVTTSMVE
                     PVADFQVNLQGTLNVLEALRRRPVPMVFASTNKVYGGLYEINLLQREPGWMPRDLAIA
                     RTGIGEQQRLDFCTPYGCSKGGADQYVLDYARQFRVPAAVLRMSCIYGPRQLGTEDQG
                     WVAHFLRQALAGEPITLYGDGRQVRDVLHVDDAVRAYVGAWQRMGTVAGQAFNLGGGP
                     SNAISLLQLIRHIESLLGRSVAVRFSDWRPNDQRWFVADPRRVRAALDLPEPQDWRTG
                     VGNLLRSFAAAGESQRAISA"
     gene            3092..4375
                     /locus_tag="VFN42_07340"
     CDS             3092..4375
                     /locus_tag="VFN42_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04295 family B12-binding domain-containing
                     radical SAM protein"
                     /protein_id="HET8996466.1"
                     /translation="MKVALLNPRWHFEHSIYFGCREPHLPLELGCAQVLLEQAGHQAL
                     LLDGHLLDLDDDALVDAVKDFGAEMVVLTTAPTYLFWRCTQPELRVPRALFDALGETG
                     ALRVAVGPHGSVTPAATLRKLGIDVVLRGECEEQIVALANSGSPARVPGAVWWNAGEP
                     VINGPPQAARFTDQPPLRWPDGWIQRHHHHHHRFDTPPVGLGAEVEASRGCPYHCTFC
                     AKIDFRDAYRRRDLQLLLEEIDGLLCQGVGYLYFIDEIFLPQKPLLEALRERDVQFGV
                     QTRIDLWKPDMLELLGAAGCVSVEAGVESLTVEGRAALDKKCRMSTDALSELLIRARR
                     SIPFVQANLIKSIGDDAGMIAEWRDMLRGQGVWANDPVPLYPYPSSPDYRRLWGDPDE
                     RAWERAHAHYLAQFDRFSDIQEDTPLPLAELERQV"
     gene            4375..5487
                     /locus_tag="VFN42_07345"
     CDS             4375..5487
                     /locus_tag="VFN42_07345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET8996467.1"
                     /translation="MDRAAPARVMLTTDSVGGVWRYCLDLATGFVARGAAVTVVVMGP
                     PANDAQRAEARICGVDLVETALPLDWLAEDAGALEQAARALAGLARELGADSVQVHSP
                     AYATGADWNVPVVAMAHSCVGTWWRAVRGDLPLPPDLAWRVDCVAHGMHAADAVIAPS
                     AAFAAMLHETYGDGHAIIAIPNGRRTAPLPRDAQPHAFTAGRLWDDAKNVALLDEAAA
                     MLEQPVRAAGPLRGPNGAQIDCRRLHLLGVLDDVQLACEYAQASVFVSPARYEPFGLA
                     VLEAAQAGAALVLSDIPTFRDLWDGAAMFVDPNDPAALALLLRRLLPSRRRCSALGVA
                     ARARAATYSAERMAAETWDVHCNLWRARETRARCAA"
     gene            5498..6607
                     /locus_tag="VFN42_07350"
     CDS             5498..6607
                     /locus_tag="VFN42_07350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8996468.1"
                     /translation="MRFVLFTHSLISCWNHGNAHFLRGVLRELLAQGHQVRSFEPEDG
                     WSRTNLVSAQGAAAASGFATRFPELVPHVRFVQPDLDAMLDGADVVLVHEWTDPALVA
                     AIGRRRAEGGRFALLFHDTHHRAVSDPDAIFRLDLSGYDAVLAFGDALADVYRRSGWG
                     DRVFTWHEAADTRLFRPPEVETQREGVVFIGNWGDDERGEELRSFLLHPAAALHLPLD
                     VYGVRYPDAALLELAAGGGHHRGWVANACVPSVFARHAMTVHVPRRFYAEQLPGIPTI
                     RVFEALACGIPLICAPWTDSEALFRPGEDYLVANSPTEMQDHMRALTADGGLRRSLAE
                     HGRETILARHTCAHRVAALMGILQRLQVAERKEAA"
     gene            6607..7701
                     /locus_tag="VFN42_07355"
     CDS             6607..7701
                     /locus_tag="VFN42_07355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8996469.1"
                     /translation="MRMAFYGSSLLSSYWNGAATYYRGLLAALARRGWQITFYEPDAF
                     DRQSHRDIDAPAWAAVRVYPATPDAVQAVLAEAAKADVVIKASGVGVFDDALLDGMMR
                     HAARQALRVFWDVDAPATLAEIRPRPEHPIRQALDGLDAVLTYGGGVPVVNAYTALGA
                     ALCRPIYNALDPSTHFPVAPDPDYATDLCFLANRMPDREARADEFFFRAAALCPERRF
                     LLGGNGWDGKPMSANVRWIGHVGTERHNAFNTSALAVLNVARESMAAMGFSPATRVFE
                     AAGAGACLITDAWEGIEQFLTPDEEILVARDGAEVAALLRDLQPDRARAIGENARQRI
                     LAEHTYERRADEVDALFRALLARKRQMAAA"
     gene            7713..8126
                     /locus_tag="VFN42_07360"
     CDS             7713..8126
                     /locus_tag="VFN42_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996470.1"
                     /translation="MRIVFLGLSLSSSWGNGHATTYRALLAGLAARGHAVTFLEREQP
                     WYASNRDLRDPAYCRLEFYHAPEDLHGFAATLRAADAVIVGSYVPDAAAVMEVLCRSL
                     GRPSVMADEGRAPRVMTGEGRAPRVMAGEGRPSTF"
BASE COUNT         1218 a         2706 c         2883 g         1404 t
ORIGIN      
        1 tctgccggga atgtcgccgg gatcagcggt gtccagatcg gccccggagc cactgcgttc
       61 acccggatgc ggcgctgcat cagctggatt gccagggacc gcgtgaacgc cacgatggca
      121 cccttggtgg atgcgtagtc cagcagcgtc ggactgcctt tataggccgt caccgacgtt
      181 gtgttcacta tcgcaccgcc atcgggcatg tgcggcagca ccgcgcggct caagaagaac
      241 tggccgaaga tgttggtacg gaatgtacgc tccaactgtt catcgctgat gtcctcgagc
      301 ttttcctgca tgtgctgttc ggcggcatta ttcacaagca cgtcaatccg gccgaactcc
      361 tgcactgcgc ggcgggcgac ttcgcggcag aaatcggagt tgccgatgtc gccgggcacg
      421 gcgattgcgc ggcgtccttc cgcttcgatc agccgcacgg tttccttggc gtcgtcgtgc
      481 tcatccaggt aggcgatcac cacgtccgca ccttctttcg cgaaggccac cgctacggct
      541 ctgccgatgc cggaatcgcc accggaaatc agcgcggcct tgccggatag cttgccggca
      601 ggccgatacc gcgccatcgc ggattgcggc cgcggctcca tctggttttc gtgtcccggc
      661 tggctttgct gatgctgcgg gggatgcggc gtgtcactca tgacggctct ccgggtttgc
      721 agcgaatgga acggcgcagc gattgccgtg ttccctttgc tgacgtcgca tacgcaagcc
      781 cgttgccgga atgattctat catggcggta caccgccgct tcgcttcaac cggctgtgac
      841 gggaacatct tctacaaacc cgagttgata cctgcagaac aggccacccc tcggtccatg
      901 ccgggcctgg gtcactgcgg aggcgcaagg ggactcgtgg aaaaacacgt gctgatcacc
      961 ggcggcgccg ggttcattgg ctgccacgtt tgcgatgcgt tgctggcgcg cggctatcgc
     1021 gtgcgcgtgc tggaccgcat gctggaacag gtgcacagca accgtccccc caatcccatc
     1081 ctgcgtgaaa ccgaattgct ggtcggcgac gtgcgtgacg cggcgttggt cggtaaggcg
     1141 ctgcgcggcg tcgatgcagt gatccatctg gctgccgaag tcggcgtcgg ccagagcatg
     1201 tacgaaatcg aacgctatgt cggcggcaac gaccagggca cggccgtact gctgcaggcg
     1261 ctgattgccc agccggtgcg ccgtgtcgtc gtcgcatcct cgatgagcat ctacggcgag
     1321 ggcctgtatc gcaccgcgga gggcgggctg gtcgatgacg cggtgcgcgg caccacggca
     1381 tgggatcccg tggatgcgca gggccgcaag ctggtgccgg tcgccacgcc ggaaagcaag
     1441 cgtgcctcgc tcgcctctgt gtacgcgatc accaagttcg tgcaggaacg cctggtgctg
     1501 aatgtggcgc cggcctacga catgcaggcg gtggcgctgc gcctgttcaa cgtgttcggc
     1561 cccggccagg ccttgtccaa tccgtatacc ggcgtactgg cgatcttcgc cgccgcattg
     1621 ctgaacggca gagcgccggc ggtgttcgaa gacggcgaac agcggcgcga cttcgtgcat
     1681 gtggaggatg tcgcgcgggc cttcgtgctg gcgctggagc accccgctgc accgggcggc
     1741 gtgttcaaca tcggcagcgg cgaaagcgtc agcatccgcg aagtcggcga gatgatcgcc
     1801 gatgcgatgg ggcgcggcga cctgcccccc accatcctgg gcaaggcgcg taccggcgat
     1861 atccgccact gctttgccga catcacgcag gcgcggcagg tgctggggtt cgagccgcgc
     1921 cgcttcctgc gcagcagcct gggtgagctc gcgaactggg tgcgccgcca gcaggccatc
     1981 gaccgcgcgg cggaagcgca gcgcgaactg gagacgcgag ggctggtggc gtgagcggca
     2041 cattttccgt cgtcggcagc cgcccgcccg tgctgatcac cggcggcgcg ggcttcatcg
     2101 gctgcaatct ggcggaccgt ctcgcgagcg atggcgagaa cgtgctggtg tacgactccc
     2161 tcgcgcgccc gggcgtggaa gcgaatcttg cctggctgcg cgctcggcat ccccgccgca
     2221 tctccagcac ggtggcggat gtacgcgacg ccgatgcggt cggcgatgcg gcgcggcaat
     2281 ccgccgcggt gttccatctc gcggcgcagg tcgcggtgac caccagcatg gtggaaccgg
     2341 tcgccgattt ccaggtgaac ctgcagggca cgctgaacgt gctggaggcg ctgcgccgcc
     2401 gcccggtgcc gatggtgttc gccagcacca acaaggtcta tggcggcctg tacgaaatca
     2461 atttgctgca gcgcgaacca ggctggatgc cgcgcgacct ggcgatcgcg cgcaccggca
     2521 tcggcgaaca gcagaggctg gatttctgca cgccgtacgg ctgttccaag ggcggtgccg
     2581 accagtacgt gctggactat gcccgccagt tccgcgttcc cgccgccgtt ctgcgcatga
     2641 gctgcatcta cggcccgcgc cagcttggca ccgaagacca gggctgggtc gcgcattttc
     2701 tccgccaagc gctggcggga gagccgatca cgctgtacgg cgacggccgg caggtgcgcg
     2761 atgtgctgca tgtcgacgac gcggtgcgcg cctatgtcgg cgcctggcaa cgcatgggca
     2821 cagtcgccgg ccaggcgttc aacctgggtg gcggtccatc caacgcgatc agcctgctgc
     2881 agttgatccg gcacattgaa tcgctgctcg gccgcagcgt ggctgtccgg ttttctgact
     2941 ggcgcccgaa cgatcagcgc tggttcgtcg ccgatccccg ccgcgtgcgg gccgcgctcg
     3001 atctgccgga accgcaggac tggcggaccg gtgtcggcaa cctgctgcgc agcttcgcgg
     3061 cggcgggtga atcacaacgg gccatctccg catgaaggtc gcgctgctca atccgcgctg
     3121 gcatttcgag cacagcatct atttcggctg ccgggagccg catctgccgc tggaactcgg
     3181 ctgcgcccag gtcctgctgg aacaggccgg ccaccaggca ttgctgctgg acggccattt
     3241 gctggacctg gacgacgacg cgctggtgga cgcggtgaag gatttcggcg ccgagatggt
     3301 ggtgctgact accgcgccga cctacctgtt ctggcggtgc acccagccgg agttgcgtgt
     3361 gccccgcgcg ctgttcgatg cgctgggcga aaccggcgcg ttgcgggtcg cggtggggcc
     3421 gcacggctcc gtcacgccgg ccgccacctt gcgcaagctg ggcatcgacg tggtgctgcg
     3481 cggcgaatgc gaagaacaga tcgtcgctct ggccaacagc ggctcccctg cccgcgtgcc
     3541 gggcgccgtc tggtggaacg ccggcgagcc ggtgatcaac ggtccaccgc aggcggcgcg
     3601 cttcaccgac caaccgccgc tgcgctggcc ggatggctgg atccagcgcc atcaccatca
     3661 tcaccaccgc ttcgacacgc cgccggtggg tctcggcgcg gaagtggaag cctcgcgcgg
     3721 ctgcccctat cactgcacct tctgcgccaa gatcgacttc cgcgacgcct accgccgccg
     3781 cgacttgcag ctgctactgg aggagatcga cgggctgctg tgccagggcg tgggctacct
     3841 gtatttcatc gacgagatct tcctgccgca gaagccgctg ttggaggcct tgcgcgaacg
     3901 cgacgtgcag ttcggcgtgc agacccgcat cgacctgtgg aagcccgaca tgctggagct
     3961 gctcggcgcc gccggctgcg tgtcggtgga agccggcgtg gaaagcctta ccgtggaagg
     4021 gcgggcggca ctggacaaga agtgccgcat gtccaccgac gcgctgagcg aattgctgat
     4081 ccgggcgcgg cgatcgattc ccttcgtgca ggccaacctg atcaaatcga tcggcgacga
     4141 tgccggcatg atcgccgaat ggcgcgacat gctgcgcgga cagggggtct gggccaacga
     4201 tccggtgccg ctatatccct accccagctc ccccgactat cgccgcttgt ggggcgaccc
     4261 cgacgagcgc gcctgggaac gtgcccatgc gcattacctc gcgcagttcg accggttcag
     4321 cgacatccag gaagacacgc cgctgccgct ggcggaactg gagcggcaag tgtgatggat
     4381 cgcgcggcgc cggcacgggt gatgctgacc accgattcgg tcggtggcgt gtggcgctac
     4441 tgcctggatc ttgcaaccgg cttcgtcgcg cgcggtgcag ccgtcacagt ggtcgtgatg
     4501 ggtccgccag ccaatgacgc acaacgcgcg gaagcgcgga tctgcggcgt ggacttggtg
     4561 gaaacggcgc tgccgctcga ctggctggcc gaggatgccg gcgcgctgga acaggccgca
     4621 cgcgcgctgg cggggctggc gcgggagctt ggcgcggatt cggtgcaggt gcattccccc
     4681 gcctacgcga ccggcgcgga ttggaacgtg ccggtggtgg cgatggcaca ttcctgcgtc
     4741 ggcacctggt ggcgcgcggt gcgcggagac ctgccgctgc cgccggacct ggcatggcgc
     4801 gttgattgcg tcgcgcacgg catgcacgca gccgatgcag tgatcgcgcc gagcgccgcc
     4861 ttcgccgcga tgctgcacga gacctacggc gacggccatg cgatcatcgc catcccgaac
     4921 ggccgccgga ccgcaccgct gccacgcgat gcacagccgc acgccttcac cgccgggcgg
     4981 ctgtgggacg acgccaagaa cgtggcactg ctggacgaag cggcggccat gctggagcag
     5041 ccggtacgcg ccgccggtcc tctgcgtggg ccgaacggcg cgcaaatcga ttgccgccgt
     5101 cttcatctgc tgggcgtgct ggatgacgtg cagctcgcct gcgaatacgc gcaggcctcg
     5161 gtgttcgtgt cgccggcgcg ctacgagccc ttcggcctgg ccgtgctgga agccgcgcaa
     5221 gccggcgccg ccctggtcct gtcggacatc ccgaccttcc gcgacctgtg ggatggcgcc
     5281 gcgatgtttg tcgatcccaa tgatcctgcg gcgctggcgc tgctgctgcg gcgcctgctg
     5341 cccagtcgcc ggcgctgctc ggcgcttggc gtggccgcac gcgcccgcgc cgcaacgtat
     5401 tcagccgagc ggatggcggc ggaaacctgg gacgtgcatt gcaacctgtg gcgcgcgcgg
     5461 gagacccgcg cgcgttgcgc agcctagcgg gagaatcatg cgcttcgtcc tgttcactca
     5521 ctcgctgatt tcctgctgga accacggcaa cgcgcatttc ctgcgcgggg tgctgcgtga
     5581 attgctggcg caaggccacc aggtgcgcag tttcgagccg gaagacggct ggagccggac
     5641 caacctggtc agcgcgcagg gcgccgccgc cgcctccggc tttgccacgc gcttcccgga
     5701 actggtgccg catgtgcgct tcgtgcaacc ggatctcgac gccatgctgg acggcgccga
     5761 cgtggtgctg gtgcatgaat ggaccgatcc ggcgctggtt gccgccatcg gccggcgccg
     5821 cgcggagggt ggacgcttcg cattgctgtt ccacgacacg caccaccgcg cggtgagcga
     5881 cccggacgcg atcttccggc tcgatctgtc cggctatgac gcggtgctgg cattcggcga
     5941 cgcgctggcc gatgtgtatc gccgctccgg ctggggcgac cgggtgttca cctggcacga
     6001 agcagcggat acgcggctgt tccgtccgcc ggaggtggag acgcaacgcg agggcgtcgt
     6061 cttcatcggc aactggggcg atgacgagcg cggcgaggag ctgcgatcgt tcctgctgca
     6121 tcccgccgcg gccttgcatc tgccgctgga cgtgtacggc gtgcgctatc cggatgccgc
     6181 gctgctggaa ctggcggcgg gcggcggaca tcatcgcggc tgggtggcga atgcctgcgt
     6241 gccgagtgtg tttgcgcgcc atgcgatgac ggtgcacgtg ccgcggcggt tctatgcgga
     6301 acagttgccg gggattccga ccattcgtgt cttcgaagct ctggcctgcg gaattccgct
     6361 gatctgcgcg ccctggaccg acagcgaggc gctgttccgt ccgggtgagg attacctggt
     6421 ggcgaattca ccgacggaga tgcaggacca tatgcgcgcg ctgaccgcgg atggcgggct
     6481 tcgccgttcg ctggcggagc atgggcggga aaccatcctg gcgcgccaca cctgcgcgca
     6541 ccgcgtggca gcgctgatgg gcatcctgca gcggctgcag gtggcggagc ggaaggaagc
     6601 ggcctgatgc gcatggcatt ctacggctcc agcctgctgt cgtcctactg gaacggcgcc
     6661 gcgacctatt atcgcggctt gctggcggcg ctggcgcggc gcggttggca gatcaccttc
     6721 tacgaaccgg acgcgttcga tcgccagtcc caccgcgaca tcgatgcgcc ggcctgggcc
     6781 gctgtacgcg tctatccggc aacgccggat gcggtgcagg cggtgctggc ggaagcagca
     6841 aaggccgacg tggtgatcaa ggccagtggc gtgggcgtgt ttgacgacgc attgctggac
     6901 ggcatgatgc gccatgccgc gcgccaggcg ctgcgcgtgt tctgggacgt ggacgcaccg
     6961 gcgactctgg cagagatccg cccgcggccg gagcatccga tccgccaggc gctggacggc
     7021 ctggatgcgg tgctgaccta tggcggcggc gtgccggtgg tgaacgccta caccgcgctg
     7081 ggtgccgcgc tgtgccggcc gatctacaac gcgctcgatc ccagcacgca ttttccggtg
     7141 gcgcccgatc cggactacgc caccgacctg tgcttcctgg ccaatcgcat gccggaccga
     7201 gaggcccggg cggatgaatt cttcttccgc gccgccgcgc tttgcccgga gcgccgcttc
     7261 cttctcggcg gcaacggctg ggacggcaag ccaatgagcg cgaacgtccg ctggatcggc
     7321 catgtcggca ccgaacggca taacgcgttc aacacctcgg ccctggcggt gctgaacgtc
     7381 gcgcgcgaga gcatggcggc gatgggcttc tctccggcga cgcgggtgtt cgaagcggcc
     7441 ggcgcgggcg cctgcctgat caccgatgcc tgggaaggca tcgaacagtt cctgactccg
     7501 gatgaggaga ttctggtggc gcgcgacggc gcggaagtcg cggcgctgtt gcgcgacctg
     7561 cagccggacc gcgcacgcgc gatcggcgaa aacgcgcggc agcgcatcct ggcggaacac
     7621 acctatgagc ggcgtgccga cgaggtggat gcgctgttcc gcgccctgct ggcgcgcaag
     7681 cgccagatgg cggcggcata ggggagcctg cgatgcgcat tgtgttcctc gggctgagtc
     7741 tcagttcgtc ctgggggaat ggacacgcca ccacttatcg cgccctgctc gccggcctcg
     7801 cggcccgcgg ccacgcggtg acgttcctgg aacgcgagca gccctggtac gcgtccaacc
     7861 gcgacctgcg ggatccggcc tattgccggt tggagttcta tcacgcgccg gaggacctgc
     7921 acggcttcgc ggcgacgctg cgggcggcgg atgcggtgat cgtcgggtcc tacgtgccgg
     7981 atgccgccgc ggtgatggaa gtgctgtgcc ggagtctggg gcgtccctcc gtcatggccg
     8041 acgaaggccg cgcccctcgt gtcatgaccg gcgagggccg cgcgcctcgt gtcatggccg
     8101 gcgaaggccg gccatccacg ttttgattgt ccgcagctgc gaaaagacgt gggtggccgg
     8161 ccgtcgccga ccatgacacg ggggagtaca ccggctcgca accgtgatta a
//
LOCUS       DASSZH010000340         4393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_587502, whole genome shotgun sequence.
ACCESSION   DASSZH010000340 DASSZH010000000
VERSION     DASSZH010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587502"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..401)
                     /locus_tag="VFN42_07365"
     CDS             complement(153..401)
                     /locus_tag="VFN42_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0106034 - protein maturation by [2Fe-2S]
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HET8996471.1"
                     /translation="MSETLPRVAQVGSYKVEVEAGKSYWWCSCGKSGKQPFCDGSHKG
                     SGFAPVEFKAEQAGIVGFCGCKQSGNKPRCDGSHKALA"
     gene            560..1618
                     /locus_tag="VFN42_07370"
     CDS             560..1618
                     /locus_tag="VFN42_07370"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HET8996472.1"
                     /translation="MRQIDVLAIPRVSAADRARIEAVDPAIRLTDAGGWFDGEVRETW
                     PAFTVRRYLPPDAYGAGSREQRDAVLAQAEVILGGWPFPLDLRARSPRLRWFHQRPAG
                     ASNLRHGDLWGSDVTVTTSRGAGNTLAMAEYTIAGMLFLAKGLHRAIVDREARDFDYR
                     AYRPWLIDGKTLCVIGAGGIGRDVGRLGAALGMRVIGTRRHPHHDAALPAGFSQLGGP
                     DDLDQFLAESDFVAVCCQWTPETTNLINADRLARMRPGSVLANVARGEIVDEAALADA
                     LARGHLRGVLLDVYNGEFTGPPPDALWRDPRVLITPHISGASDESRHGAIDVFCENLR
                     AYLDGKPLQNVIDWTLGY"
     gene            1748..2494
                     /locus_tag="VFN42_07375"
     CDS             1748..2494
                     /locus_tag="VFN42_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8996473.1"
                     /translation="MTFDFSGKRAVVCGGSRGIGRAIALGFAQAGGAVSICARGAETL
                     ERTRQEIAAFGHPAHAASCDLGDAAAIRGYSADAAAALGGIDLLVNNASAFGSSDDEA
                     GWMSSMAVDMMAIVHATQAALPFLQQSKGSVVNTSSIAAMHTAARQPPYGAIKAAVIH
                     YTGTQAAMYAKDGIRVNCIAPGSIEFPGGVWDRRKTEAPKLYDAVLRSIPFGRLGYPE
                     EVANVALFLASPLASWVTGQTIAVDGGQLL"
     gene            2684..3592
                     /gene="cysD"
                     /locus_tag="VFN42_07380"
                     /pseudo
     CDS             2684..3592
                     /gene="cysD"
                     /locus_tag="VFN42_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
     gene            3592..>4393
                     /locus_tag="VFN42_07385"
     CDS             3592..>4393
                     /locus_tag="VFN42_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET8996474.1"
                     /translation="MPESLLRVLACGSVDDGKSTLLGRLLFECGSIPDDVMAALEQDS
                     RRFGTVEGGIDYALLVDGLSAERDQGITIDVAYRFFETPRRRFILADAPGHQQYTRNM
                     VTGASTSDVAIVLVDARKGVLDQTRRHAAIASLLGIRDIVLAVNKMDLVDFDPEVFER
                     IVADFHAVLGRLKARSVTAIPVCARDGDNVTTESPRMPWYQGMTLLGALENAEPGHDG
                     ASRPFRMPVQLVNRPDHTFRGYAGTIASGTVRVGSTILNPAARTEAKVA"
BASE COUNT          693 a         1495 c         1477 g          728 t
ORIGIN      
        1 gagattgctg ttgccggccg tggcggtgcc agtgtgatag ttggttgccg ggatgttgtc
       61 ccaggtgaag atggtgaggg agacgtcatc gattttcatg gcacttccgt cccgtgccgt
      121 ggccggggtc agcccggcca cggcgattga ggctaggcca gggccttgtg cgagccgtcg
      181 caacgcggct tgttgcccga ctgcttgcag ccgcagaagc cgacaatgcc ggcctgttcc
      241 gccttgaact ccaccggcgc aaatccggaa cccttgtgcg agccgtcaca gaacggctgc
      301 ttgccgctct tgccgcagga gcaccaccag tatgacttcc ccgcttccac ttccaccttg
      361 taggaaccca cctgcgcgac gcgcggcagt gtttcggaca tgcgtgtttc tccccatttg
      421 cagcattgcg gccggcgccc gacgcgcccc cgacaccagc gtatccgccc ggtctggccg
      481 cggctagacc cggtatggtt tgcgcggcca aggcatccgt gctttgcgct atgctccgct
      541 gcaatcggga ggtggttcaa tgcgacagat cgacgtgctc gccatcccgc gcgtcagcgc
      601 tgccgaccgc gcccggatcg aagcggttga tcccgccata cgcctgaccg atgccggagg
      661 ctggttcgac ggcgaagtcc gggaaacctg gccggcattc accgtcaggc gctacctgcc
      721 gccggatgcc tacggtgccg gctcgcgcga gcaacgcgat gcagtgctgg cgcaggccga
      781 ggtgatcctg ggcgggtggc ccttcccgct ggacctgcgc gcacgttcgc cccggctgcg
      841 ctggttccac cagcgcccgg ccggcgccag caacctgcgg catggcgatc tgtggggcag
      901 cgatgtcacc gtcaccacct cgcgcggtgc cggcaacaca ctcgccatgg cggaatacac
      961 catcgccggc atgcttttcc tcgccaaggg cctgcatcgt gccatcgtgg accgcgaggc
     1021 tcgggatttc gactatcgcg cctatcggcc atggctgatc gacggcaaga cgctgtgcgt
     1081 gatcggggcc ggcggcatcg gccgcgatgt cggtcggctt ggcgccgcgc tcggcatgcg
     1141 ggtgatcggc acgcgccgcc atccgcacca cgatgcggcg ctgcctgctg ggttctccca
     1201 gctcggcggg ccggatgatc tcgaccaatt cctcgcggaa agcgatttcg ttgcggtgtg
     1261 ctgccaatgg acgccggaga ccaccaacct gatcaacgcg gaccgcctgg cacggatgag
     1321 acccggcagc gtgctggcca acgtggcgcg cggcgagatc gtggatgagg ccgcactggc
     1381 cgatgcgctg gcccgcggcc atctgcgcgg cgtgctgctg gacgtttaca acggcgagtt
     1441 caccggtccg ccacccgatg cattgtggcg cgacccgcgc gtgctgatca cgccgcatat
     1501 ttccggcgca agtgacgaaa gccggcatgg cgcgatcgat gtgttctgcg aaaaccttcg
     1561 cgcgtatctg gacggcaagc cgctgcagaa cgtgatcgac tggacgctgg ggtactgacg
     1621 cgcggcaagc cgcggaaggc ttggcgggcg cgggccggtg ccctgccggc aggcatagcg
     1681 gacggagtcc ctccccatcg ccgccgcgct gtgctggtgt agcggcctgc gcacagggag
     1741 agactccatg acgtttgatt tttccggcaa gcgggcggtg gtctgcggcg gcagccgcgg
     1801 catcggccga gcgatcgccc ttggcttcgc acaggcgggt ggggcggtgt cgatctgcgc
     1861 ccgcggtgcg gagacgctgg agcgcacgcg gcaggagatc gccgccttcg gccatcccgc
     1921 gcatgccgca tcctgcgacc tgggggatgc cgcggcgatc cgcggctaca gcgccgacgc
     1981 ggccgccgcg ctgggtggca tcgacctgct ggtgaacaac gcctctgcct tcggttccag
     2041 cgacgatgag gccggctgga tgtccagcat ggcggtggac atgatggcga tcgtgcacgc
     2101 cacgcaggcg gcgctgccgt tcctgcagca gagcaagggc agcgtggtga acacgtcctc
     2161 gatcgcggcg atgcacaccg cggcacggca gccgccctac ggcgcgatca aggccgcggt
     2221 gatccactac accggcaccc aggcggcgat gtatgccaag gacggcattc gggtgaactg
     2281 catcgcgccg ggctcgatcg aattccccgg cggcgtatgg gatcggcgca aaaccgaggc
     2341 cccgaaactg tacgatgccg tactccgatc tatcccgttc gggcggcttg ggtatccgga
     2401 ggaggtcgcg aatgtcgcgc tgttcctggc gtcgcccctg gcgtcctggg tgaccggcca
     2461 gaccatcgcg gtggacggcg gccagttgct gtagccccgc cgcacaaccc ctgcccggcg
     2521 aggacccctc ctccgcaagg aatggtgggt ttaccctttc tttccggtcg ccacctctat
     2581 aggcactgag gcaatgccgc attgccgccg ggctcgcttc cggcggaccg gcagggtgtg
     2641 acagcgggct cggcgcccgg caggggacta tggcgaaccg gcagagactg accgtatgac
     2701 agcgcacctc aaggcgctcg aagccgaaag catccatatc ctgagggaag tcgccgccca
     2761 ggccgcgcgc ccggtgttgc tgtattccat cggcaaggac tcctcggtcc tgctgcatct
     2821 ggcgctcaag gccttctacc cggcgcgtcc gccctttccg ctgctgcaca tcgacacgac
     2881 gtggaagttc cgcgagatga tcgccttccg cgacgccacg gcggagcggt acggactcga
     2941 cctccgcgtc cacaccaacc aggaggggct gcgccagggc atcaatccgt tcgaccacgg
     3001 cagcagcatc tatacgcagg taatgaagac cgatgcgctg aaacaggcgc tgaccgaggg
     3061 ccgctacgat ttcgccattg gcggcgcccg gcgcgatgag gagcggtctc gcgccaagga
     3121 acgggtgttc tcggtgcgcg gcccgggaca cacctgggaa ccgaagcgcc aacggccgga
     3181 actctggcac ctgtacaacg gcagcctggc tcccggcgag acgctgcgcg tgtttccgct
     3241 gtcgaactgg tccgaaggcg acgtgtgggc ctacatccag gcagaaggca tcgacgtggt
     3301 gccgctgtat ttcgctgccc agcgccccac cgttctgcgc gacggcgcgc tgatcgtggt
     3361 ggatgacggc cgcatgcggt ttctccccaa cgagcacccg gaactgcgca tggtccgctt
     3421 tcgctcgctc ggctgctacc cgctctccgc cgcggtggaa tccaccgcgg cctcgatcgc
     3481 cgacatcgtg caggaaatgc gcacgacccg ggtctccgag cgcgccggcc ggctgatcga
     3541 ccaggaccaa gacgcttcca tggaacagaa gaagcgcgag ggatatttct gatgcccgag
     3601 tcgctcctgc gcgtgctggc ctgcggcagc gtcgatgacg gcaaatccac gctgctcggg
     3661 cgcctgctgt tcgagtgcgg cagcatcccg gatgacgtga tggcggcgct ggaacaggac
     3721 tcccgccgtt tcggcacggt ggaaggtggc atcgactacg cgctgctggt ggatggcctg
     3781 tcggccgaac gcgaccaggg catcaccatc gacgtcgcct accgcttctt cgaaacgccg
     3841 cgccgccgct tcatcctggc ggatgcgccc ggccaccagc agtacacccg caacatggtc
     3901 accggcgcgt caacctcgga tgtagcgatc gtgctggtcg acgcccgcaa gggcgtgctg
     3961 gaccagaccc ggcgccacgc cgccatcgcc tcgctgctcg gcatccgcga catcgtgctg
     4021 gcggtgaaca agatggacct ggtcgatttc gacccggagg tgttcgagcg cattgtcgcc
     4081 gacttccacg ccgtgctcgg gcgcctcaag gcgcggtcgg tgacggcgat tccagtttgc
     4141 gcccgcgacg gcgacaatgt caccactgag agcccgcgga tgccttggta ccagggcatg
     4201 acgctgctcg gggcgctgga gaacgcggaa cccgggcatg acggcgcatc acggcccttc
     4261 cgcatgccgg tgcagctggt caatcggccg gaccacacct tccgcggcta cgccggcacg
     4321 atagcgtccg gcaccgtgcg cgtgggcagc accatcctca accccgcggc gcgcactgag
     4381 gcgaaagtcg cgc
//
LOCUS       DASSZH010000341         6190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1473974, whole genome shotgun sequence.
ACCESSION   DASSZH010000341 DASSZH010000000
VERSION     DASSZH010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6190
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473974"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VFN42_07390"
     CDS             <1..715
                     /locus_tag="VFN42_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8996475.1"
                     /translation="DAMPHGGDLRISCAEAALQDDPELSAGRYVRLTVSDQGVGMSED
                     VLARIFEPFFTTKPAGKGTGLGLSQVYAVVHQANGAVRVSSRVGAGTTVEILLPVAAD
                     PAATNGTESEVPQMRPRPASVLVVDDDPDVRSVAVAMLEALGCRVTEAPDGVTALALI
                     ETQAPELLLLDFAMPGMTGAELARRVRERHPDLPIVFATGYSDSEAMHQAVGRNAPVL
                     RKPFRMDQLQSMLSEVLDR"
     gene            complement(1133..2317)
                     /locus_tag="VFN42_07395"
     CDS             complement(1133..2317)
                     /locus_tag="VFN42_07395"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET8996476.1"
                     /translation="MTTALPIAQPENVGLSPDALHRLGAALQSRVDRGHIPGAVALVA
                     RHGKVAYHQAFGVRDPDNGAPMQTDAIFRIYSMTKPIVSVAVMMLWEEGRLLLSDPAG
                     QYIPEFAASKVAVLRDGGMDTVPAAAPITVQDLLRHTSGLTYEFRGATPVHKAYMEAN
                     IARRKQTNAEQASTLAGLPLLHQPGTRWEYSRSTDVLGRLVEVVSGQSLGEFLQQRVL
                     APLGMADAAFWVPEAQQGRLAEAFRRDPDTGTEVNLLDVRRPAMFESGGGGMVATAMD
                     YARFLQMLLGGGKLDAAQLLGRKTVEFMTADHLGDIPSAPDLVPPGHGFGLGFAVRKQ
                     TGVAPFPGSVGTFYWGGAAGTTFWVDPVERLAAVLMIQAPVQREHYRVLFRDLVYAAL
                     TD"
     gene            2518..2814
                     /locus_tag="VFN42_07400"
     CDS             2518..2814
                     /locus_tag="VFN42_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HET8996477.1"
                     /translation="MSSRLVNRNVVAERGRTSIRLEPELWDALLEICHREDQDLNAIV
                     RQVEAAGHSGGRTSAVRVFVLGYYRSAATEAGHLAAGHGPHTRPRLDAVSRTAA"
     gene            complement(2819..3103)
                     /locus_tag="VFN42_07405"
     CDS             complement(2819..3103)
                     /locus_tag="VFN42_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HET8996478.1"
                     /translation="MARIHPGRVLREEYMEPMRLDEAGLARALDVQPMKIAALVKDHA
                     AITSDLALRLARCFRTSPQFWLSLQAAYDLAVAQAEHGAEIEARVQPLAA"
     gene            complement(3257..3547)
                     /locus_tag="VFN42_07410"
     CDS             complement(3257..3547)
                     /locus_tag="VFN42_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996479.1"
                     /translation="MSTNQAEAPGPQAHDDESGPPGALDRLIHETVECLTAIRNYAAG
                     ARRALAGGAPEQADPALVKIDAQVYRAHDLLGKARQINNGDRPSGQARPPPV"
     gene            complement(3802..>6190)
                     /locus_tag="VFN42_07415"
     CDS             complement(3802..>6190)
                     /locus_tag="VFN42_07415"
                     /inference="COORDINATES: protein motif:HMM:NF024795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HET8996480.1"
                     /translation="LEISRSSGGPIDLQLDPTQTYTPNQFHVGTIGTNTAVGQSVPCY
                     LAGTRIRTETGEMPVEQLRVGDRVITRDGSAKPIRWIGRRSYSGAVPAGLRDVEPVAI
                     AAGALADGVPARELIVSPLHALYLDGVLVPAERLVNGRTIRRRPDIDPIRYFHIELER
                     HDVLFAEGAPAESFVDCDSRSMFHNAAEYDALYPGEPAERWLFCAPRVESGEALAAIR
                     RRLDERAGLAPEAPDAAPGPLLGRLDGVEAGRIAGWAFQPDRPDRPVWLEVLANEGVI
                     ARVEARRYRADLEAAGIGDGRHGFELNLTGRLGQAQVIRVRRVSDGAELDGSPLALRP
                     LAADGLADEARRLVATAVLDPDDASTLDAFAAELLRGIDTLRQARVQAAAPVRASRRV
                     QRRALIIDSALPRADRDAGSQAVLCHAAALNELGWQVEFVAPHALADFAADAEALEAA
                     GYVVHRAPQVASVEEVLRRNRNGFELVYLHRLANAEAYAPLARQWQARARVIYSVADL
                     HHLRVGRQADVQSCEELAAAAMALRVRETNAMRLADAVITHSSAEAAYLADLAPGAVV
                     HVVPWVPLTRPSPVPFGARQGVACIGGWMHEPNVDAVRWLTASIMPLVWERAPRIALL
                     VVGSAWPESVPWIADPRIRVVGQVGSLDPLLSLVRLTVAPLRFGAGLKGKVLDSFAAE
                     VPVVMTPVAAEGLALEGAARGLVGEGAEALAALILRAHEDAAFNRAAGLAGRRLAETQ
                     FTADAVKAALQRAVARPGVRESGSPPAVTWGLTSPAHVRPESLVATRDQHPVQAQ"
BASE COUNT          983 a         2213 c         2054 g          940 t
ORIGIN      
        1 cgatgccatg ccgcatggcg gcgatcttcg catcagttgc gccgaagcgg cgctacagga
       61 tgatccggaa ctttccgccg gccgctatgt gcggctcacc gtcagcgacc agggcgttgg
      121 aatgagcgag gacgtgctgg cgcgcatctt cgagccgttc ttcaccacca agcctgcggg
      181 caaggggacc ggacttgggc tgagccaggt ctatgccgtc gtgcatcagg caaacggcgc
      241 ggtccgcgtg tcgagccggg tcggagccgg aacgacggtg gagatcctgc tgccggtcgc
      301 ggcggacccg gccgcgacaa acgggaccga atctgaggtc ccgcagatgc gtccccgtcc
      361 cgcctccgtg ctggtggtcg acgacgatcc cgacgtacgc agcgtcgccg tcgccatgct
      421 ggaggcgttg ggatgccgtg tcacggaagc ccctgacggc gtaaccgcgt tggcactgat
      481 cgagacgcag gcgcccgaac tcctgctgct cgatttcgcc atgccgggca tgaccggggc
      541 cgaactggca cggcgggtac gggagcgcca tcccgatctg ccgatcgtgt tcgccaccgg
      601 atactccgat agcgaggcga tgcaccaggc tgtgggcagg aacgcgcccg tgctgcgcaa
      661 gccgttccgc atggatcagc ttcagagcat gctgtcggaa gtgctggatc gctgacgcgg
      721 tttgccgccg gccgcgacgt cctggcggac ccaaaccgct tatactagcc cgccttgctg
      781 atgacacacc gccagcagta ccgcgtcgtg gcgggcgcag gcccgccacc cacgactttc
      841 ctgatcgcaa ttgaggaaag tcgtggatgc cggcctgcgc cggcatgacg gaattcggcg
      901 tgccgaagcg tcgagctcat cgcgggttgg tattaggcga cacgcataaa taggggattc
      961 ggctggcggc acgctgctcc gttccgtcat ggtcggcggg aggcaccatg acgaggtggc
     1021 acaagccggc cggtgcgtcg tggttattcc tgcgcgtcgc cttatcgcac tagagacagt
     1081 agcggctttc ggcgaaggac agccgagccc tgtgggagct cggcgatccc gctcaatccg
     1141 tcagcgcggc atagaccaga tcgcggaaca gcacccggta atgctcgcgt tgcaccggcg
     1201 cctggatcat cagcacggcg gcgagccgtt cgaccggatc gacccagaag gtcgtccccg
     1261 ccgcgccgcc ccaatagaac gtgccgaccg acccagggaa cggcgccact ccggtctgct
     1321 tgcgcacggc aaagcccagg ccgaacccat gccccggggg caccagatcc ggcgcgctgg
     1381 gaatatcccc gaggtgatcg gcggtcatga actcgaccgt cttgcgcccc agcagctgtg
     1441 ccgcatccag tttgccgccg cccagcagca tttgcaggaa acgggcgtag tccatggcgg
     1501 tggccaccat gccgccgccg ccggattcga acatcgccgg acgccgcacg tccagcagat
     1561 tgacctcggt gccggtgtcc gggtcacgcc ggaacgcttc cgccagccgg ccctgctgcg
     1621 cctccggcac ccagaacgcc gcgtccgcca tgcccagcgg cgccaacact ctctgttgca
     1681 ggaattcgcc caggctctgg ccggaaacca cctccaccag gcgacccagc acgtcggtgg
     1741 agcggctgta ttcccagcgc gtgccgggct gatgcagcaa cggcagcccg gccagcgtgc
     1801 tggcctgctc cgcgttggtc tgcttccgcc gcgcgatgtt cgcctccatg tacgccttgt
     1861 gcaccggcgt ggcgccgcgg aattcatagg tcagtccgga ggtgtggcgc agcaggtcct
     1921 gcacggtgat cggcgccgcc gccggaacgg tgtccatgcc gccgtcgcgc agcacggcca
     1981 ccttggacgc ggcgaattcc gggatgtact ggccggccgg atcgctgagc aacagccggc
     2041 cttcctccca cagcatcatc accgcgaccg acacgatcgg cttggtcatg gagtagatac
     2101 ggaagatcgc atcggtctgc atcggcgcgc cgttgtccgg atcgcgcacg ccgaaggcct
     2161 gatggtacgc caccttgccg tggcgggcca ccagcgccac cgcgccaggg atgtggcctc
     2221 gatccacccg gctctgcagc gcggcgccca gccggtgcag cgcgtccggc gaaaggccga
     2281 cattctccgg ttgagcgatg ggcagtgctg tggtcatgac ggctcctggc tgcggtttca
     2341 cccccacagc atggcacgcc tgcggcccgc cgtagagggc acagcccctg atgccgaggc
     2401 ggcgcgacgt aaaatagtat gcgaataccc cgcactgctt ttcttgccgg cgcaaacctt
     2461 ctcttaagct ggatgtggtc ctctgcgcgc attgcatttg ggacggaggc agccgtggtg
     2521 agcagtcgac tggtgaaccg caacgtcgtt gccgaacgcg gccggaccag catacggctg
     2581 gaaccggagt tgtgggacgc gctgctggaa atctgccatc gggaggacca ggacctcaac
     2641 gccatcgtgc gtcaggtgga ggcagcggga catagcgggg gccgtaccag cgcggtgcgg
     2701 gttttcgtgc tgggctatta ccgcagcgcg gcgaccgagg ccggacattt ggcggccggc
     2761 catggtccgc acacccgtcc gcggctggac gcggtctcca ggaccgcggc ctgacggttc
     2821 aggcggcgag cggctggacc cgtgcctcga tttccgcgcc gtgctcggcc tgcgccaccg
     2881 ccaggtcgta ggcggcctgc agggacagcc agaattgcgg gctggtgcgg aagcagcgcg
     2941 ccaggcgcag cgcgaggtcg gaggtgatgg cggcgtggtc cttgaccagg gccgcgatct
     3001 tcatcggctg aacatccagt gcgcgcgcca gcccagcctc atcgagccgc atcggctcca
     3061 tgtattcttc acgcagcacg cgtccagggt ggatacgagc cataagcgcc gcctgtggga
     3121 ttgctatggc aaagagcaga caccagaaca gtggcagcaa gaaggcatct gctggtgtcg
     3181 cctggggcag cggcctcggc gtcggctcgc gtgcgcgaag gcgcagttca cccgccccat
     3241 tgtggccaag gggctttcag accggagggg gccgcgcctg tccggatggg cggtcgccat
     3301 tgttgatctg ccgcgccttc cccagcagat catgcgcgcg gtacacctgc gcatcgatct
     3361 tgacgagggc agggtccgcc tgctccggcg caccgccagc caacgcccgg cgagcgccgg
     3421 cagcgtagtt gcgtatggcg gtgaggcact cgacggtctc atgaatcagc cggtccagcg
     3481 cgcccggcgg cccggattca tcgtcatgcg cctgaggccc cggcgcctca gcctgattgg
     3541 tgctcattgt ctgaacgccc ggccaatgga agctgccagg caggctaatg gtacgtttac
     3601 gcactggcaa attgccttgg gcaaacggcg ttccgcctcg ccgtcgcgcc gatcttcagt
     3661 gcctgttcac gttgttgcgc atcgccccga tacatagtgg ctctggacgg caattatctt
     3721 caaaacatgc tgccgactga gacagtcccc agatcgcccg ccgtcagtat gcggacggat
     3781 ttggcatttc tattccagct ctcattgagc ctggaccggg tgctgatcac gcgtcgccac
     3841 aaggctttcc ggcctgacat gggcgggaga cgttaacccc caggtcaccg ccggcggtga
     3901 tccggactca cggacacccg gccgagcgac ggcgcgctgc aaggcagcct tcactgcgtc
     3961 cgcggtgaac tgcgtttcgg ccagccgccg tcccgccaag ccagccgcgc ggttgaatgc
     4021 cgcgtcctca tgcgcgcgca ggatcaaggc cgccagggcc tcggccccct cacccaccag
     4081 cccccgtgcc gcgccttcca gtgcgagtcc ctccgccgca accggcgtca tcaccaccgg
     4141 cacctcggcg gcgaagctgt ccagcacctt gcctttcagc ccggcaccga accgcagcgg
     4201 cgccaccgtg agccgcacca gcgacagcag cggatcgagc gacccgactt gccccaccac
     4261 gcgtatgcgc gggtcggcaa tccacggcac ggactccggc caggcgctgc ccaccaccag
     4321 cagtgcgatt cgcggcgccc gctcccagac cagcggcatg atgctggcgg tcagccagcg
     4381 caccgcatcg acgttcggct cgtgcatcca gccgccgatg caggccacgc cctggcgggc
     4441 gccgaatggc accgggctgg gacgcgtcag cggcacccag ggaaccacgt gcaccaccgc
     4501 gcccggcgcc agatctgcca ggtacgccgc ttccgcgctg gaatgggtga tcaccgcatc
     4561 ggccagccgc atggcgttgg tctcgcgcac ccgcaacgcc atcgccgccg cggcgagttc
     4621 ttcgcaggac tgcacgtcgg cctggcggcc aacccgcaga tgatgcaggt ccgcgacgct
     4681 gtagatcacc cgtgcgcgcg cctgccattg gcgcgccagc ggcgcatagg cctccgcatt
     4741 ggccagccgg tgcaggtaca ccagctcgaa gccattgcgg ttgcggcgca gcacctcctc
     4801 caccgaggcg acctgcggcg cgcggtgcac gacatagccc gcggcctcca gcgcctcggc
     4861 atcggcggca aaatccgcca gcgcatgcgg cgcaacgaat tccacctgcc agccgagctc
     4921 gttcagcgcc gcggcgtggc agagcaccgc ctgcgagccc gcatcgcgat ccgcccgggg
     4981 cagcgccgag tcgatgatca gcgcccggcg ctgcacgcgc cgtgacgccc gcaccggcgc
     5041 ggcggcctgc acccgcgcct gccgcagcgt gtcgatgccg cgcagcaatt cggcggcaaa
     5101 cgcgtcgagg gttgacgcat cgtccggatc cagcacggca gtggcgacca gccgccgcgc
     5161 ttcatccgcc aagccatccg ccgccagcgg gcgcagcgcc agcggcgatc catccagttc
     5221 cgccccgtcg gaaacccggc ggacgcggat cacctgcgcc tgccccaacc ggccggtgag
     5281 gttcagctcg aagccgtggc gcccgtcgcc gatgccggca gcctccaggt cggcgcgata
     5341 acgtctggcc tcgacccggg cgatcacccc ctcattggcc agcacctcca gccagaccgg
     5401 ccgatccggg cgatcgggct ggaaggccca gccggcgatc cgcccggcct ccaccccgtc
     5461 cagccgcccg agcagcggac ccggtgcggc gtccggcgcc tccggcgcca ggccggcccg
     5521 ttcgtcgagg cggcgccgga tcgcggcgag cgcctcgccg ctctccaccc gcggcgcgca
     5581 gaacagccag cgctcggcgg gttcgccggg atacagcgcg tcatactccg ccgcgttgtg
     5641 gaacatcgac cggctgtcgc aatcgacgaa gctttccgcc ggcgcgccct cggcgaacag
     5701 cacgtcgtgc cgttccagct cgatatggaa gtagcggatc gggtcgatat ccggccgccg
     5761 gcggatcgtc cggccattca ccagccgctc cgccggcacc agtacgccgt ccaggtacag
     5821 cgcatgcaac ggcgagacga tcagctcccg cgccgggacg ccatcggcca aggcgccggc
     5881 ggcgatcgcc accggctcca cgtcgcgcag cccggccggc acggcaccgg aatagctgcg
     5941 ccggccgatc cagcggatcg gcttcgcgct gccgtcacgg gtgatgacgc ggtcgccgac
     6001 ccgcagctgc tcgaccggca tctcgccggt ctcggtgcgg atgcgcgtgc ccgccaagta
     6061 gcagggcacg ctctgcccca ccgcggtgtt ggtgccgatc gtccccacat ggaactggtt
     6121 cggggtgtag gtctgggtgg gatcgagctg aaggtcgatc gggccgccgc tgctgcggct
     6181 gatctccagc
//
LOCUS       DASSZH010000342        12554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_843104, whole genome shotgun sequence.
ACCESSION   DASSZH010000342 DASSZH010000000
VERSION     DASSZH010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 12554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12554
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843104"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..499)
                     /locus_tag="VFN42_07420"
     CDS             complement(68..499)
                     /locus_tag="VFN42_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HET8996481.1"
                     /translation="MRSRQSLDAPEALVLTMVLVAAADGGMTDREIGVMAGQVQTLPA
                     FQDFTTEQLREATDAAVGLLNEEDGLQHAARLIRAALNRRLRETAYALACEVIAADQV
                     ASQESLQMLEFVQSELDLDPLVAAAIERGVRARYRRVESGN"
     gene            complement(550..3339)
                     /locus_tag="VFN42_07425"
     CDS             complement(550..3339)
                     /locus_tag="VFN42_07425"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HET8996482.1"
                     /translation="MAAGHGRAAILTPDGELLLLPSEAAAERLRSLPPPLLIHAPASF
                     RRLGLRPGPAFDLLELFAFVLPARTAAPTPRGLALALDYDPPLSGLEADAGLLPDLAA
                     ALLHRLAQGRDTALNREAPELAARMGAAGWGWAPFVLAALGRPEARPSADSLRVWRRL
                     PEWEDAAPLPPPSAHPVTEAEARQRLATMLGPQAEQRPAQADYAGAAAAAFAPREVKG
                     APHLVLAEAGTGTGKTLGYLAPASVWAEKNRGTVWISTFTRHLQRQIQSELARLYPDP
                     AERRGKVVIRKGRENYLCLLNLDDAVRAATSGFIQTQSIPLALIARWALATEDGDIQG
                     GDLPGWLSELYGAGFLAALADRRGECIHAACTHWRRCFVEHSIRRARGAQLVVANHAL
                     VMTQAAWGGLDDTTVPTRYVFDEGHHVFDAADGAFAAALTGLETAELRRWLRGAEGGR
                     SRARGLRRRVEELVADRPELEAPLDAALQAALALPAPDWRERLGEEGPELSGIEAGRS
                     NPTETLLRLMRRQLLARLGPGERQHAWGSMECDLFPVSPELVQAAEALDRAYGRIAEP
                     LARLRERLLARLDDEAEELDAATRSRIEAIGRSLARRALNPLAAWQAMLRHMAGAEPS
                     LGTLPAYVFFLQIERRQDRQDLDVGLHRHWLDPTVPFAATLAAPAHGLVVTSATLRDA
                     GVEADPEAAWDAAEARVGAPHLPHPAIRAAVPSPFDYAAQTRALIVHDVAAGDIKHLA
                     AAYRALFVAAGGGGLGLFTAIHRLRAVHARIAPELEAAGIPLYAQHVDAMGNATLVDI
                     FRTEEESCLLGTDAMRDGVDVPGRALRLVVFEKVPWPRPDILHRERRTHLSGGDPKGF
                     DDRIARLRLRQAFGRLIRRSSDRGVFVLLDRQTPSRLLSSFPPGVAVRRVGLAEAVKE
                     TRAFLNTASMSQSGA"
     gene            complement(3424..4710)
                     /locus_tag="VFN42_07430"
     CDS             complement(3424..4710)
                     /locus_tag="VFN42_07430"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HET8996483.1"
                     /translation="MDASRVAAIRAAVAAQRDFVQRLFDGLREDGRDEPGVSRDPYGA
                     GEQRAHARIAAAGAARDLAIERDAAANLYLTQPGRDRTAPRVVIGSHLDSVPHGGNYD
                     GAAGVVAGLAAIAALRQAGVTPACDVSVMGIRAEESIWFQVSYIGSRGALGTLPDGAL
                     DVRRIDTGRTLAEHIAECGGDPAALRAGRRFLDPAMLRAYLELHIEQAPSLLAAGKPV
                     GICTGIPGNFRYPDAWIEGSHDHVGLPRRFRRDAAMAGAEFAMALDTVWEEHESRGVP
                     MACTIGRFHTDPAMHGLTIVPGAFHFSLDVRAYDEAVLAELDRKVDAIIAGIEQRRGV
                     RFHRGPKARAAVGPVDPAIRRALEAGADVLGIAAMPLGSPASHDAAAFAAAGVPMGMI
                     FVRNENGSHNPREAMTLDDFLAGTTLLAWWLAETLG"
     gene            complement(4767..5528)
                     /locus_tag="VFN42_07435"
     CDS             complement(4767..5528)
                     /locus_tag="VFN42_07435"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET8996484.1"
                     /translation="MASASVAYETTYPAGDATAAAPNRLKLTPLLQGLDGRIISALAG
                     CTQEVSLPEDHVLCRQGAEPQHLHVLLEGQVALWGTAPDGSSAVVEVIRPPGYFVLAT
                     VLTRLPYMMSARTVAPSRLLMIEVEGLHALLQREVGLANALLRAEALNFRSLVRQVQD
                     LKLRTAAQRLGCYLLALTNDSEAKIADFRLPFDKRLLAARLGCRQENLSRAFASLRAV
                     GVETHGSRVILHDIKALREFAVPDQHFDPELPFSG"
     gene            6000..6974
                     /locus_tag="VFN42_07440"
     CDS             6000..6974
                     /locus_tag="VFN42_07440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HET8996485.1"
                     /translation="MRAMVCERFGGPEVLAQRELPDPPPPGPGEVAVRIRARGVQYVD
                     VLMLAGKYQFRPEPPFVPGGEAAGEVVAVGPGVTAFRTGDRVMSRHTLGAFAELGNAK
                     AELCDPVPDGMDLEAAGVFRAAYSTAYHALLQRGRLKSGEWVLVHGAAGGIGIAAIQV
                     AKLFGAKVIATASTEAKRAACVEEGADHAIAYSGGFLDAVKALTGGRGVDVVYDPIGA
                     QVTEESLRCLAWGGRLLVLGFLGGGPATIRSNYLLIKGIDVVGVRIGGLNEAAPELAI
                     ANMRALTRLAAEGKLKPRISHRLPLERAAEALQAVIDRAVIGKAVLVS"
     gene            7748..7927
                     /locus_tag="VFN42_07445"
     CDS             7748..7927
                     /locus_tag="VFN42_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996486.1"
                     /translation="MTLTRMLQTCRNWLSRRGEKRRAAERATAEQRDLQRKQMLMTQL
                     SGMLESGRRNGSKPR"
     gene            8349..9374
                     /gene="dctP"
                     /locus_tag="VFN42_07450"
     CDS             8349..9374
                     /gene="dctP"
                     /locus_tag="VFN42_07450"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HET8996487.1"
                     /translation="MRPVLTRRAFGAAAAATLATPFISRAEAAAPIELRCSLDTAPSH
                     PRNVAFRDFLGKIEAASGGKIKTKLFESGALFPDLHVVKALVQGQVEMCCPGTWTVTG
                     FVPDADFSQLPALYGLPIDTVHKATDGSAGKFVNDEVSKRLHVDLLGGWFDLGFNNWF
                     STKKALKSLESLKGMKLRSPGGILNSYRIRFFGGIPNVTAWPDVPLALSQGTFDGLIS
                     TDESVNSAKLFDSGIRHTLQDHQGMGLYVPMMNQNFAQKIGPKMKDEIMKIWSENLAT
                     WRANTAKSQEHGLEELKTHGVEVVYVSDAERKSVREKLLQGQDKAAHEAHMSPKLVKL
                     VMADIGA"
     gene            9367..9951
                     /locus_tag="VFN42_07455"
     CDS             9367..9951
                     /locus_tag="VFN42_07455"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HET8996488.1"
                     /translation="MPDADPARDALARFMRAWDWVERTIVGLLGLVAMITAAVQVFGR
                     YIDPANAITWAEEVIVYIIVWAIMIIASQLVRHDGHVRPDLVLRLMHPRRQRWVEMFN
                     CLVAIVFMAGLVYYGKQVVDTALLLSQTSSSDLQFPMWIYYTSLPVGAGLMLVRYLIR
                     LYRYAFHFDPKTMSVGHNISHEAPADLTVISTVE"
     gene            9966..11246
                     /locus_tag="VFN42_07460"
     CDS             9966..11246
                     /locus_tag="VFN42_07460"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HET8996489.1"
                     /translation="MFTAGFLVLFFGLLASGMPIFLVLGACAAVLYYISGEPMIGAAQ
                     NMIDQLNSSTLMSLPLFVMAAAFMRRGGVAQALVDLSTAWLGGIKGSLGLVAVVSCAL
                     FAAISGSSVATALAMGTILLPAMLERGYPRSFALGVLGASGTIGVVIPPSLALILYGI
                     VTEQSVPRLFLAGILPGLLQAGAFFLWVLWYARYKDFPAEPKMEFSRRLRVTARALPA
                     MAVPFVVLVGIYGGFMTVTESAALAAVTALVVSLFVYRGFPIRRTLSVIAEALSSAGV
                     IMLIIATALVFGHWMTGSGIPGRLVQWAAKSGFSTWEFLLLMNVLLLIIGCFLEVVST
                     LLLVMPILAPVLVPLGVDPVHFGIIFTHNMEVGLVHPPVGLNLFVLSTISDAPIGEVI
                     RGIFPFLILLLIVLGLITYWPAMTLWLPDLVFGK"
     gene            complement(11409..>12554)
                     /locus_tag="VFN42_07465"
     CDS             complement(11409..>12554)
                     /locus_tag="VFN42_07465"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HET8996490.1"
                     /translation="GAFNDTQSQALGGHVIAAAVQRAGVAPEQVDDVVMGAALQQGAQ
                     GSNMARQCALRAGLPDSVAGMTVDRQCSSGLMAIATAAKQIIDDGMEVVVGGGLESIS
                     LVQTPQMNRTRGQDPVLVQRVPALYMSMLETAEIVADRYNISREAQDAYALQSQQRTA
                     AAQQAGRFDAEIVPMPAEMVVTDRNTGETSRKLVTLAKDEGNRPDTTAEGLASLKPVF
                     TEGQRVKGGRFITAGNASQLSDGASAAVLMEAKEAERRGLQPLGIYRGMAVAGCAPDE
                     MGIGPVFAVPKLLAKHGLKVDDIGLWELNEAFACQVLYCRDRLGIPDERLNVDGGAIS
                     IGHPYGMSGARMTGHALIEGKRRGAKYVVVTMCVGGGMGAAGLFEVA"
BASE COUNT         1936 a         4353 c         4233 g         2032 t
ORIGIN      
        1 gggggccgac cgcacgaccc ctccccccaa ccccctccca caaggggagg gggagtctgg
       61 tggttattca gttgccgctc tccacgcggc gataacgggc acggacgccg cgctcgatgg
      121 ccgcggccac cagcggatcc aggtccagct ccgactggac gaactccagc atctgcagcg
      181 actcctggct ggccacctga tccgcggcga tcacctcgca ggccagcgca taggccgtct
      241 cgcgcagacg ccggttcagc gcggcgcgga tcagccgggc ggcatgctgc aggccgtcct
      301 cctcgttcag caatcccacc gcggcatcgg tcgcctcgcg caactgctcg gtggtgaagt
      361 cctgaaacgc cggcaaggtc tgcacctggc cggccatgac gccgatctcc cggtcggtca
      421 tgccgccgtc ggcagcggca accagaacca tggtcaggac cagggcttcc ggggcatcca
      481 aggattggcg ggaacgcatc gtatcggctc cataggttcg cttgagcctg agcgaagcgc
      541 ggcaccggat caagcgccgc tctgcgacat tgacgcggtg ttcagaaacg ctcgcgtctc
      601 tttcaccgcc tcggccaagc cgacccggcg gaccgccacg ccgggaggaa acgacgacag
      661 caggcgcgac ggcgtctgcc ggtccagcaa cacgaaaacg ccgcgatcgg aggaccggcg
      721 gatcaggcgt ccgaaggcct gccgcagccg cagccgcgcg atccggtcgt cgaatccctt
      781 gggatcgccg ccggacaggt gggtgcggcg ttcccgatgc aggatatcgg gacgcggcca
      841 cggcaccttc tcgaacacca ccagccgcag cgcccgcccc ggaacatcga cgccgtcgcg
      901 catcgcgtcg gtgcccagca ggcagctttc ctcctcggtg cggaagatgt cgaccagcgt
      961 cgcgttgccc atggcatcga catgttgggc ataaagcggg atgccggccg cctccagctc
     1021 cggggcgatg cgcgcatgca ccgcgcgcag ccggtggatc gcggtgaaca ggcccagccc
     1081 gccaccgccc gcggcgacga acagggcgcg ataggcggcc gccagatgct tgatgtcgcc
     1141 ggccgcgacg tcatgcacga tcagtgcccg cgtctgcgcc gcgtagtcga agggggaggg
     1201 caccgcggcg cggatcgccg ggtgcggcag atgcggtgcg ccgacccgcg cctccgcggc
     1261 atcccaggcc gcctcgggat cggcctccac cccggcgtcg cgcagcgtgg cggaggtgac
     1321 caccagcccg tgcgccggcg cggccagcgt cgccgcgaag ggcacggtcg ggtccagcca
     1381 atgccggtgc aggccgacat ccagatcctg gcggtcctgc cgccgctcga tctgcaggaa
     1441 gaacacatag gcgggcagcg tgccgagcga tggttcggca ccggccatgt ggcgcagcat
     1501 cgcctgccac gccgccagcg ggttcagcgc ccgccgcgcc agcgaacggc cgatcgcctc
     1561 gatccggctg cgcgtcgcgg catccagttc ctcggcctca tcgtccagcc gcgccagcag
     1621 gcgttcgcgc aaacgggcca atggttcggc gatacggcca taggcgcgat ccagcgcttc
     1681 cgcggcctgc accagctcgg gactcaccgg gaacaggtcg cactccatgc tgccccaggc
     1741 atgctggcgc tcgcccgggc ccaggcgcgc cagcagttgc cggcgcatca gacgcaagag
     1801 ggtttcggta ggattggacc ggccggcctc gatgccggag agttccggtc cctcctcgcc
     1861 cagccgctcg cgccagtccg gcgccggcag cgccagcgcg gcctgcagcg cggcatccag
     1921 cggcgcttcc agctccgggc ggtcggccac cagctcctcg acccggcggc gcaggccgcg
     1981 ggcgcgcgac ctgcccccct ccgcaccgcg caaccagcgg cgcaactccg cggtctccaa
     2041 cccggtcagt gccgcggcga aggcgccgtc cgcggcatcg aacacgtggt ggccttcgtc
     2101 gaacacgtag cgggtcggca ccgtggtgtc gtccagcccg ccccaggccg cctgggtcat
     2161 caccagcgca tgattggcca ccaccaactg cgcgccgcgg gctcggcgga tcgagtgttc
     2221 cacaaagcag cgccgccaat gcgtgcaggc ggcgtggatg cactcgccac ggcgatccgc
     2281 cagcgcggcg aggaagccgg caccgtacag ttcggacagc cagccgggca ggtcgccgcc
     2341 ctggatatcg ccgtcctcgg tggccagcgc ccagcgcgcg atcaatgcca gcgggatgct
     2401 ctgggtctgg atgaaaccgg aggtggcggc acgcaccgcg tcgtccaggt tcagcaggca
     2461 gaggtagttc tcccgcccct tgcggattac caccttgccg cggcgttccg ccgggtccgg
     2521 atacaaccgc gccagttcgc tttggatctg ccgctgcaga tggcgggtga aggtgctgat
     2581 ccacacggtg ccgcggttct tctccgccca gacgctggcc ggcgccaggt agccgagtgt
     2641 cttgccggtg ccggtgccgg cctcggccag cacaaggtgc ggggcaccct tgacctcacg
     2701 cggggcaaag gcggcggcgg cggccccggc gtaatccgcc tgcgccggcc gctgttcggc
     2761 ctgtggcccc agcatggtgg ccaggcgctg gcgggcttcc gcctcggtca ccggatgcgc
     2821 cgaaggcggc ggcaagggcg cggcgtcttc ccattccggc agccggcgcc agacccgcag
     2881 cgaatcggcg gaggggcggg cctccggccg gcccagcgcc gccaggacga agggcgccca
     2941 gccccagccg gcggcaccca tgcgcgcggc cagctccggg gcctcgcggt tcagcgcggt
     3001 atcgcggccc tgcgccagcc ggtgcagcag cgcggcggcg aggtcgggca gcagcccggc
     3061 atccgcttcc agcccggaga gcggcgggtc gtagtccagc gccaacgcca gaccgcgcgg
     3121 ggttggggcc gcggtgcggg cgggcagcac gaaggcgaac agttccagca gatcgaaggc
     3181 gggtccgggc cgcaggccca gccggcggaa gctggccggc gcatggatca gtagcggcgg
     3241 cggcaggctg cgcagccgct ccgccgcggc ctcggagggc agcagcaaca gctcgccgtc
     3301 gggcgtcagg atggcggcac ggccatgccc ggctgccacg gcaggagcgt cggggaggac
     3361 gaggctggtg cgcaccagcg acgggtagcg cggatcgccg ccgcgttcca gcgaagccgc
     3421 ggttcagccc agggtctcgg cgagccacca ggccagcagc gtcgtcccgg cgaggaagtc
     3481 gtccagcgtc atggcttcgc gcgggttgtg gctgccgttc tcgttgcgca cgaagatcat
     3541 gcccatcggc acgcccgccg cggcaaaggc cgccgcatca tgcgaagccg ggctgcccag
     3601 cggcattgcc gcaatcccca gcacatccgc accagcctcc agtgcccggc ggatcgccgg
     3661 atcgaccggc ccgaccgccg cccgcgcctt ggggccgcgg tggaagcgca cgccgcggcg
     3721 ctgctcgatg ccggcgatga tggcgtcgac cttgcggtcg agttccgcca gcaccgcttc
     3781 gtcataggcc cgcacgtcca gcgagaaatg gaaggcaccg ggcacgatgg tcaggccgtg
     3841 catcgccgga tcggtgtgga accgcccgat cgtgcaggcc atcggcacgc cgcgcgattc
     3901 gtgttcctcc cacaccgtgt ccagcgccat ggcgaactcc gcaccggcca tcgcggcgtc
     3961 gcggcggaag cggcgcggca ggccgacatg gtcgtggctg ccttcgatcc aggcatcggg
     4021 atagcggaaa ttgccgggaa tgccggtgca gatacccacc ggcttgccgg ccgccagcag
     4081 gcttggcgcc tgctcgatat gcagttcgag ataggcccgc agcatggccg gatcgaggaa
     4141 gcgccggccg gcgcgcaatg ccgccggatc gccgccgcat tccgcgatgt gctccgccag
     4201 cgtgcgcccg gtatcgatgc ggcggacgtc cagcgcgcca tccggcaagg tgccaagcgc
     4261 gccgcggctg ccgatatacg aaacctgaaa ccagatgctt tcctcggcgc ggattcccat
     4321 cacgctgacg tcgcaggctg gcgtcacgcc ggcctgccgc agcgcggcga tcgcggccag
     4381 gccggcgacg acgcccgcgg cgccgtcgta attgccgcca tgcggcacgg agtcgagatg
     4441 cgaaccgatc accacgcgcg gtgcggtgcg gtcgcgtccc ggctgcgtga gataaagatt
     4501 cgccgcagca tcgcgttcga tcgccagatc gcgtgcggcg ccggctgcgg cgatccgtgc
     4561 atgcgcacgt tgttcgccgg cgccgtatgg atcgcgcgat acgccaggct cgtcgcgtcc
     4621 atcctcgcgc aggccatcaa acaaccgctg tacgaaatcg cgctgggcgg ccaccgccgc
     4681 gcggatcgct gctacccggc ttgcgtccat atgcctgatt ccccgcttgc cagccggcag
     4741 ttacagccgg ccggcggggc gcgcaatcag ccggagaagg gcagttccgg atcgaagtgc
     4801 tggtctggca ccgcgaattc ccgcagcgcc ttgatgtcgt gcaggatcac ccgggatcca
     4861 tgcgtctcga cgccgaccgc acgcaacgag gcgaaggcgc gcgacagatt ctcctgccgg
     4921 cagccgagcc gagcggcaag caggcgcttg tcgaacggca gccggaaatc ggcgatcttc
     4981 gcctccgagt cgttggtcag tgccagcaga tagcaaccga gccgctgcgc cgcagtgcgc
     5041 agtttgaggt cctgcacctg gcgcacaagc gaccggaagt tcagcgcctc ggcgcgcaac
     5101 agtgcgttgg ccagccccac ttcgcgctgc agcaatgcat gcaggccttc gacttcaatc
     5161 atcagcaggc gcgacggcgc gacggtgcgt gcgctcatca tgtacggcag gcgggtcagc
     5221 acggtggcca gtacgaagta acccggcgga cggatgactt ccaccacggc gctggaaccg
     5281 tccggggcgg tgccccacaa ggcaacctgg ccttcgagca gcacgtgcag atgctgcggt
     5341 tccgccccct gccggcacag cacatggtct tccggcaggc tgacctcctg cgtgcaaccc
     5401 gcaagagcgc tgatgatccg gccatccagt ccctgcagca gcggcgtcag cttcaaccgg
     5461 ttcggcgcag cggctgtcgc atcccccgct gggtaagtgg tctcatatgc gacgctcgcg
     5521 cttgccatgg cctgcctcat accggagatt cttcgccaag acggaatagg ctctaatgtt
     5581 gacctgaatc aagagtattc cgtagaccgg ttgcacggtc ttggcagcaa gcggctaaaa
     5641 tcctggtcac actttggatg aagatgaact tcttcttagc acagcctgtc gttttcgtaa
     5701 tgtcatgatg cgacgccgcc tgtttcgccc gcacggccta ggctcttgca gccagactgt
     5761 atctttcact ggtttatgcc actgctgtcc gcagctcttt tctttgccgg ttcttacgcc
     5821 gcggtgctga acggcgctcc tgccgcggcc ggccttcgcg acggcggctt gcagcgcaca
     5881 agcggtcacg cattgttgtg tgcggcacgc ctgccgccgg tggcccggcc ggcagcgctc
     5941 acccgcttcg cagcgccgtc acggtgcggt taactgtcgg ccagcagagg agacgcgaga
     6001 tgcgggcaat ggtgtgcgag cgcttcggtg ggccagaggt tctggcgcaa cgcgagctgc
     6061 cggatccgcc gccaccggga ccgggcgaag tggccgtgcg cattcgcgcc cgaggcgtgc
     6121 agtacgtcga tgtgctgatg ctggccggca aataccagtt ccgtcctgaa ccgccattcg
     6181 tgccgggtgg cgaggccgcg ggcgaggtgg tggccgtcgg ccccggcgtc actgcgttcc
     6241 gtaccgggga ccgggtgatg agccgccaca cgcttggcgc gttcgccgaa cttggcaatg
     6301 ccaaggcgga gctgtgcgat ccggtgccgg acggcatgga cctggaggcc gcgggcgtgt
     6361 tccgggccgc ctacagcacc gcgtatcacg cgctgctgca gcgcggccgg ctgaagtccg
     6421 gcgaatgggt gctggtgcac ggcgccgcag gcggcatcgg catcgccgcg attcaggtgg
     6481 cgaaactgtt cggtgccaag gtgatcgcca ccgccagcac cgaggcgaag cgtgccgcct
     6541 gcgtcgagga aggtgccgac cacgccatcg cctattcggg cggattcctg gatgcggtga
     6601 aggcgctgac cggcggccgg ggggtggacg ttgtgtacga tccgatcggc gcgcaggtca
     6661 ccgaggaatc gctgcgctgc ctggcgtggg gcggacgctt gctggtgctg ggtttcctgg
     6721 gcggcgggcc agcaaccatc cgcagcaact atctgctgat caagggcatc gacgtggtgg
     6781 gggtgcggat cggcgggctg aacgaggccg cgccggaact ggccatcgcc aacatgcgtg
     6841 cgctgacgcg gctggcggcc gaaggcaagc tgaagcctcg catttctcat cggctgccgc
     6901 tggaacgtgc ggcagaggcc ttgcaggcgg tgatcgaccg cgcggttatc ggcaaggccg
     6961 tgctggtgag ctaacgccgt ccgagcgcgt ctgaccgtgc gtacaacatt tggttgctgc
     7021 cggccggcac gccgcgaaga gcgcggcgct ggtacttcgg aaggccgcgc ggtgcgcggc
     7081 gccggtgctc agaacggcgg caattcgtcg cgcgaatcca gccgccattt ccagccgggt
     7141 ggcgcaacgg gaagcgcagc accttcaaac acgggaacgc gggccacggt tgccattccc
     7201 gcggcgttca ggagtgcata agtaccagta acctgggcca aatcgcccgg cgaaaataca
     7261 gccggaagtt gcatacgcct ttctccactc actctcagcg ctccatcccc atgtcagcga
     7321 tcacgaatcg ccgttgttgt tcaagcattc gtgaacattc tgtctactcc gatcaacgcc
     7381 gcggcgcaaa ctgcattcgt cgcccggccg aactgctgga acgtcgtggc gcctttgcca
     7441 cagcgcggca tgtgagttgg cggcagccga tcgcaagcgc gacggcagcg ggtcgcggga
     7501 ttgttcctgt tgatgatcga aagcgcgcag tctcgtccga cgggacagcg cgagactgcg
     7561 gcgagcggga tggcttgccc ggaaaccgct tgcccccgcg ctgcagccgc ggaaacgttg
     7621 cgtacccggc gttttgcttc gacgcaggat tccgctgcat cctcgcctga agcgctggcc
     7681 cgggggcaac cgatcacttt ctgctcacca tgcatccagg agccgcgtgc tgcgttgcag
     7741 cgccgccatg accctcaccc ggatgttgca gacttgccgg aattggctca gccgccgcgg
     7801 ggagaaacgc cgcgcggcgg agcgcgccac cgccgagcag cgggaccttc agcgcaagca
     7861 gatgttgatg acgcagttgt cgggcatgct ggaaagcggc cggcggaacg gctccaagcc
     7921 gcgctgagtg caatcgcggg ttgcacctcg gattgcggaa tgatggtcat cccccgtcgc
     7981 agcggttcgg gcgtggcgcc ctgagtggcg aagccgcgcg tgacccgcct tcgatgccgc
     8041 cggcaacctg atgcagggtc cgggctgatc ggttctggtc ctgcgccggc tccggcgtcg
     8101 catccgtcct cgggcgtcga attcgcccga gccgtcgcgg cacggcttgg caacaggccc
     8161 accgtggggc cgcgagacgg ggccattgca ccgcgcggag gtggcgccgg cgggcggtcc
     8221 caccgggcaa ccggcgatgc cgcacgccgg ctcgaccgat tccgcagaca aatgatgacg
     8281 ctgccttgac gagtgtgaag tcaaagtgct ctagggacgc gctaaacagt gttctgggag
     8341 cttaagggat gcgtcctgtc ctcacccgcc gcgccttcgg cgccgccgcg gccgccactc
     8401 tcgccacccc cttcatcagc cgggccgaag ccgctgcccc tatcgagctg cgctgctcgc
     8461 tcgatacggc gccgtcccac ccgcgcaacg tcgcgttccg cgacttcctg ggcaagatcg
     8521 aggcggcctc cggcggcaag atcaagacca agctgttcga aagcggtgcg ctgttccccg
     8581 acctgcacgt ggtgaaggcg ctggtgcagg gccaggtgga gatgtgctgc ccgggcacct
     8641 ggacggttac cggcttcgtg ccggacgccg acttctccca gttgccggca ctctacggcc
     8701 tgccgatcga caccgtgcac aaggccaccg acggctccgc cggcaagttc gtcaatgacg
     8761 aagtctccaa gcgcctgcat gtcgacctgc tcggcggctg gttcgacctg ggcttcaaca
     8821 actggttctc caccaagaag gcgctgaagt cgctggagtc cctgaagggg atgaagctgc
     8881 gcagcccggg tggcatcctg aactcctatc gcatccgctt cttcggcggc attcccaacg
     8941 tcaccgcctg gccggacgtg ccgctggcac tgtcgcaggg caccttcgat ggcctgatca
     9001 gcacggacga aagcgtcaac agcgccaagc tgttcgattc cggcatccgc cacacgctgc
     9061 aggatcatca gggcatgggc ctctacgtgc cgatgatgaa ccagaacttc gcccagaaga
     9121 tcggcccgaa gatgaaggac gagatcatga agatctggtc cgagaatctg gcgacctggc
     9181 gcgccaacac ggccaagtcg caggagcacg ggctggagga actgaagacg cacggcgtcg
     9241 aggtcgtgta tgtctccgac gcggagcgca agtccgtgcg cgagaagctg cttcagggcc
     9301 aggacaaggc ggcgcacgag gcacacatgt cgcccaagct ggtcaagctg gtgatggcgg
     9361 atatcggtgc ctgacgctga cccggcgcgc gacgcgcttg cgcgcttcat gcgggcctgg
     9421 gattgggtcg agcggaccat cgtcggtctg ctcggcctgg ttgccatgat caccgccgcc
     9481 gtccaggtgt tcggccgcta tatcgacccg gcgaacgcca tcacctgggc ggaggaggtg
     9541 atcgtctaca tcatcgtctg ggcgatcatg atcatcgcca gccagctggt ccgccacgat
     9601 ggccacgtgc gcccggatct cgtgctgcga ctgatgcacc cgcgcagaca gcgctgggtg
     9661 gagatgttca actgcctggt ggcgatcgtt ttcatggcgg ggctggtcta ttacggcaag
     9721 caggtggtgg acaccgcgct gttgctgagc cagaccagtt cgtccgacct gcagttcccg
     9781 atgtggatct actacacctc gctgccggtc ggcgccggcc tgatgctggt gcgttacctg
     9841 atccggctgt accgctacgc tttccacttc gacccgaaga ccatgtccgt gggacacaac
     9901 atttcccacg aagcaccggc tgatctcacc gtaatatcca ccgttgaata aggcgggctg
     9961 gcgcgatgtt cacggccgga ttcctggtgc tgttcttcgg cctgcttgcc tccggcatgc
    10021 cgatcttcct ggtgctcggc gcctgcgccg cggtgctgta ctacatctcg ggcgagccga
    10081 tgatcggcgc cgcccagaac atgatcgacc agctgaactc ctccacgctg atgtcgctgc
    10141 cgctgttcgt catggcggcg gccttcatgc ggcgcggcgg cgtagcccag gcgctggttg
    10201 acctgtccac cgcctggctg ggcggcatca aaggatcgct gggtctggtc gcggtggttt
    10261 cctgcgccct gttcgccgcg atctccggat cctcggtcgc gaccgcgctg gccatgggta
    10321 ccatcctgct gccggcaatg ctggaacgcg gctatccgcg ctccttcgcg ctcggcgtgc
    10381 tgggcgcctc cggcaccatc ggcgtggtaa tcccgccgtc gctggcactg atcctgtacg
    10441 gcatcgtcac cgagcagtcg gtgccgcggc tgttcctcgc cggcatcctg ccgggcctgc
    10501 tgcaggccgg cgcgttcttc ctgtgggtgc tgtggtacgc gcggtacaag gactttcccg
    10561 ccgaaccaaa gatggaattc agccgccgcc tgcgggtgac cgcacgggcg ctgccggcca
    10621 tggcggtgcc tttcgtggtg ctggtcggga tctacggcgg cttcatgacg gtgacggaat
    10681 ccgccgcgct ggccgcggta accgccctgg tggtcagcct gttcgtctat cgcggcttcc
    10741 ccatccgccg cacgctgtcg gtgatcgccg aggctctgtc cagcgccggc gtcatcatgc
    10801 tgatcatcgc caccgcgctg gtgttcggcc actggatgac cggttccggc atccccggac
    10861 gtctggtgca gtgggccgcg aagtccgggt tcagcacctg ggaattcctg ctgctgatga
    10921 acgtgctgct gctgatcatc ggctgcttcc tggaggtggt cagcacgctg ctgctggtga
    10981 tgccgatcct ggcgccggtc ctggtgccgc tgggcgtcga tccggtgcat ttcggcatca
    11041 tcttcaccca caacatggaa gtgggcctgg tgcatccgcc ggtcgggctg aacctgttcg
    11101 tgctctccac catttccgac gcgccgatcg gcgaggtgat ccgcgggatc ttcccgttcc
    11161 tgatcctgct gctgattgtg ctgggcctga tcacctactg gccggcgatg acgctctggc
    11221 tgccggatct ggtgttcggg aagtagcaga ctcctccccc tccccttgtg ggagggggtg
    11281 gggggagggg tcgtgcgtga gttcccaggg accgatcgcg cgccccctcc ccctgccctt
    11341 ggtccacttc gcggcccacg ctcacaaggg gagggggaaa gagtcagggc cctgccgtca
    11401 aggcattatc aggcgacttc gaacagcccc gcggcgccca tgccgccgcc cacgcacatc
    11461 gtcaccacca cgtatttcgc cccgcgccgc ttgccctcga tcagtgcgtg gccggtcatg
    11521 cgggcgccgg acatgccata tggatggccg atggaaatcg cgccgccgtc cacgttcagc
    11581 cgctcatcgg gaatgcccag ccgatcgcgg caatacagga cctggcacgc aaaggcctcg
    11641 ttcaactccc acaggccgat gtcatccacc ttcaggccat gcttggcgag cagcttcggc
    11701 accgcgaaga ccgggccgat gcccatttcg tccggcgcgc agccggccac cgccatgccg
    11761 cgatagatcc ccagcggctg caagccgcgg cgttcggctt ccttcgcttc catcagcacc
    11821 gccgccgatg cgccgtccga cagttgcgac gcgttgccgg cggtgatgaa gcggccgccc
    11881 ttcacgcgct ggccttcggt aaacaccggc ttcagcgacg ccaagccctc ggcggtggtg
    11941 tccggccgat tgccctcgtc cttcgccagc gtcaccagct tgcgtgacgt ctcgccggtg
    12001 ttgcggtcgg tcaccaccat ctcggccggc atcggcacga tttccgcatc gaaccgcccg
    12061 gcctgctgcg ccgccgcggt gcgctgctgc gactgcaacg cgtatgcgtc ctgggcctcg
    12121 cgggaaatat tgtagcggtc ggcaacgatt tcggcggttt ccagcatgct catgtacagc
    12181 gcgggaacgc gctgcaccaa cacggggtcc tggccacgcg tgcggttcat ctgcggcgtc
    12241 tgcaccagcg agatgctctc cagtccgccg ccgaccacaa cctccatgcc gtcatcgatg
    12301 atctgcttgg ccgcggtggc gatcgccatc aatccggacg aacactgccg gtccacggtc
    12361 atgccggcga cgctgtccgg cagtcccgca cgcagcgcgc attggcgagc catgttcgaa
    12421 ccctgggcgc cctgctgcag cgcggcgccc atgacgacgt cgtccacctg ctccggcgcg
    12481 acgccggcgc gctgcaccgc ggcggcaatg acgtggccgc ccagcgcctg ggactgcgtg
    12541 tcgttgaacg cgcc
//
LOCUS       DASSZH010000343         3584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_161655, whole genome shotgun sequence.
ACCESSION   DASSZH010000343 DASSZH010000000
VERSION     DASSZH010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161655"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="VFN42_07470"
     CDS             <1..1109
                     /locus_tag="VFN42_07470"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HET8996491.1"
                     /translation="GYYEQRWPEYVPVAERRRRAELAMEKLRKKGHPVAPVTLESKKI
                     ATTFWGKAWCDNLEGYADFASRIERGRTYVRNGSVVDLQIAPREVTAIVSGSELYNVK
                     ISISEVAKPHWARICRDCTGGIDSLVELLQGRFSNGVMERICRQQDGLFPRPAEIRFT
                     CSCPDWADMCKHVAAVLYGIGARLDQQPELLFRLRAVDENALVAGINATVPLSKGGPA
                     ADKVLAEDDLSALFGLDMEQREAAPEPNEPVARGAEKRSSASRKKPLAKPAKPVVAKP
                     AQARAATKETAPPTPAVKRGRPIAAGPVSPSEPVKSRAKPASAPASRRASDPPTSADG
                     KVQRARGAQRPLAKPTKSGGGAARLRPLAAGSKA"
     gene            complement(1397..1621)
                     /locus_tag="VFN42_07475"
     CDS             complement(1397..1621)
                     /locus_tag="VFN42_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996492.1"
                     /translation="MSLASAAQLWSTPGMGKFDGIASLAARIIRSARQQATGGLARSG
                     EVAQRVKAEAVTRRGMMDAALREKRAKRGK"
     gene            complement(1729..2874)
                     /locus_tag="VFN42_07480"
     CDS             complement(1729..2874)
                     /locus_tag="VFN42_07480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8996493.1"
                     /translation="MTGAVPDVVVIGGGLHGCSAALHLAKRGARVTVLEKDYVGRHAS
                     GVNAGGVRRLGRHFAEVPLSVASLELWYRIRDLVDDDCGFEHHGQVRVAENEAEFATL
                     HERAAELNRLGFDHEEVIDQAELRRLVPAIAQHCIGGLVSRADGAANPYRTTMAFKRK
                     AESCGVRFVEGARVTTLRRSGRHWSVEAQDGTRWEAPVLVNCAGAWADRIAAQLGEPV
                     PLEVWALMLMISAPMPRFLEPVIGAAGRPLSFKQYANGTVLVGGGYKGRPQRDENWTD
                     LDFRGLAQSARTVWDLFPIMRSAQIVRSWAGIEGRLPDDIPVIGPSSTEEGVFHAFGF
                     AGHGFQLGPVVGSITAELVSTGSSNLPIAPFRIDRFRAPALFQPSGV"
     gene            complement(2876..>3584)
                     /locus_tag="VFN42_07485"
     CDS             complement(2876..>3584)
                     /locus_tag="VFN42_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET8996494.1"
                     /translation="TEVAYQAGNGTEQRIDADVVLLHQGVAPNLNLANAIGCRQEWQE
                     SQLCFAPVVDAWGASSVDGVAIAGDGAGIGGARIAAERGRLAALDAAYRLGRIDAAKR
                     DGEAGACRHALQRFARGRRFLDLLYRPRPEHRIPSGDTLVCRCEEVTARQILDTVKLG
                     CSGPNQMKSFLRCGMGPCQGRLCGLTVTELIAQARGMTPGEIGYYRLRPPIKPITLEE
                     LASLPKNDAAVNAVVRS"
BASE COUNT          622 a         1209 c         1187 g          566 t
ORIGIN      
        1 gggggtatta cgagcagcgg tggccggaat acgtccctgt cgcggaacgg cggcgccgcg
       61 ccgagctggc gatggaaaag ctgcgcaaaa agggccaccc cgtcgcgccg gtgacactgg
      121 agagcaagaa gatcgccacg acgttctggg gcaaggcgtg gtgcgacaac ctggaaggct
      181 acgccgattt cgccagccgc atcgaacgcg ggcgcaccta tgtgcgtaac ggctcggtgg
      241 tggatctgca gattgcaccg cgcgaggtga cggcaattgt cagcggttcc gagctgtaca
      301 acgtcaaaat cagcatctcc gaagtggcga agccgcactg ggccaggatc tgccgcgact
      361 gcaccggcgg catcgactcg ctggtggaac tgctgcaggg gcgcttttcc aacggggtga
      421 tggagcgcat ttgccggcaa caggacggcc tgttccccag accggccgaa atccgcttca
      481 cctgcagctg tccggattgg gccgacatgt gcaagcacgt cgcggcggtc ctctacggca
      541 tcggcgcccg gctggaccag cagccggaat tgctgttccg gctgcgtgcg gtggacgaga
      601 acgcgttggt cgcggggatc aacgcgacgg ttccgctgtc gaagggcgga cctgccgcgg
      661 acaaggtgct ggcggaggat gatctgtccg cgctgttcgg gctggatatg gagcagcgag
      721 aagcggcacc cgaaccaaac gaaccagttg ctcgcggcgc ggagaagcgc agttcggcca
      781 gccggaagaa gccgctcgcc aagccggcca agcccgtggt tgcgaaaccg gcccaagcca
      841 gagcggcaac caaagagact gcgccaccaa cgcccgcagt aaagcgcggc cggccgattg
      901 ccgccggccc ggtcagtcct tctgaaccgg tgaaatcccg agcgaagccc gcgagcgctc
      961 cagccagccg gcgagcctct gatccgccca catccgcgga cggcaaggtt cagcgtgcgc
     1021 gcggtgctca gcgaccgctc gccaagccga ctaaatcggg cggcggggct gccagactcc
     1081 gtccgctggc ggcagggtcg aaggcataag ggcaaggtcc ggcctggctt tgtagaccgc
     1141 ctcaaggcga aagagcgccc ggttcgtgcg ggtgatcgcc tccctggcgc cggccattgc
     1201 ctgccgcgcc aacaggatgg aattctggtg ggaatccagg gcgcttggtc caacgaatgg
     1261 ggtcatggcc ggctgagtgc gttccaaagg agcaattcga atactaatta gccgaccatc
     1321 cgcagaatgg ctgtgagcta gctcacagca tcagcgcctg ccctgggtgc caagccgtct
     1381 gctgccgtcg ccggagctac ttgccgcgtt tggcgcgctt ctctcgcagg gcggcatcca
     1441 tcatgccgcg gcgggtcacg gcttccgcct tgacgcgctg cgcgacctct ccggagcgtg
     1501 cgagaccacc cgtcgcctgc tgccgcgcgc tgcggatgat ccgggccgcg agagacgcga
     1561 tgccatcaaa tttgcccatg ccgggagttg accacagctg ggcggcgctg gccaacgaca
     1621 tcgcggaatg tcccgtggag ccgacgcacg ggcaggccaa agcccgtggc cggcaggcgc
     1681 aggccgccat ccgcggctgc ctggggacgg ctgccaaacg ggcttggatc aaacaccgga
     1741 tggctggaat aaagctggcg cgcggaagcg atcgatacgg aagggagcga tcggcagatt
     1801 gctgctgccg gtgcttacca attctgcggt gatgctgccg accaccgggc cgagctgaaa
     1861 accgtggccg gcgaaaccga aggcatggaa cacaccttct tcggtggagc tgggtccgat
     1921 caccgggatg tcgtccggca ggcgtccctc gatccccgcc caactgcgca cgatctgtgc
     1981 cgaacgcatg atcgggaaca ggtcccagac agtgcgcgcg ctctgggcaa ggccacggaa
     2041 atccaggtcg gtccagttct cgtcccgctg gggccgcccc ttgtagccgc caccgaccag
     2101 gaccgtgccg ttggcgtatt gcttgaacga caacggccgc cccgcggcac cgatgaccgg
     2161 ctccaggaaa cgcggcatgg gtgcactgat catcagcatc agtgcccaga cttccagcgg
     2221 cacgggctcg ccgagctgcg cggcgatgcg gtccgcccag gcgccggcgc agttcaccag
     2281 caccggcgcc tcccatcgtg tgccgtcctg tgcttccacg ctccagtgcc ggccgctgcg
     2341 ccgcagcgta gtcacgcgcg cgccttccac gaagcgcaca ccacagcttt ccgctttgcg
     2401 cttgaacgcc atcgtggtgc ggtacgggtt ggccgcaccg tcggcacgcg acaccagtcc
     2461 gccgatgcaa tgctgtgcaa tcgccggcac cagccggcgc agttccgcct ggtcgatgac
     2521 ttcctcgtgg tcgaagccca accggttcaa ctccgcggcg cgttcgtgga gcgtggcgaa
     2581 ttccgcctcg ttctccgcca cgcgcacctg gccgtggtgt tcgaatccgc aatcatcgtc
     2641 caccagatcg cggatccggt accacagttc cagtgacgcg accgacaagg gcacttcggc
     2701 gaaatggcgg ccgaggcggc gcacgccgcc ggcattcacg ccagaagcat ggcggccgac
     2761 atagtccttc tccagcaccg tgacgcgcgc gccacgcttc gccagatgca atgcggcgga
     2821 gcagccgtgc aggccgccgc cgatgacgac gacgtcgggc actgcgccgg tcacgtcagc
     2881 tccgcaccac ggcgttcacc gcggcatcgt tcttcggcag actcgccagc tcttccaagg
     2941 tgatcggctt gattggtgga cgcaggcggt aatagccgat ttcgcccggt gtcatgccgc
     3001 gcgcctgtgc aatcagttcg gtgaccgtca ggccgcacag ccggccctgg cacggtccca
     3061 tgccgcagcg caggaacgac ttcatctggt tcggcccgga acagccaagt ttcaccgtgt
     3121 cgaggatctg ccgcgcggtg acctcctcgc accggcacac cagcgtgtcg ccggatggga
     3181 tgcgatgttc tgggcgtggc cgatacagca gatcgaggaa gcggcggccg cgcgcaaagc
     3241 gctgcaatgc gtgtcggcag gcgccggcct cgccatcgcg ctttgcggca tcgatgcgtc
     3301 ccagccggta tgctgcgtcc agtgccgcca gccttccccg ctcggctgcg atccgcgcgc
     3361 cgccgatgcc ggcgccatcg ccggcgatgg caacgccatc gacgctgctg gcgccccatg
     3421 catccacaac cggcgcgaag cacagttgag attcctgcca ttcctgccgg cagccgattg
     3481 cgttggcgag gttcaggttg ggcgccacgc cctgatgcag caacaccaca tcggcgtcga
     3541 tccgctgttc ggtgccgttg ccggcttgat aggccacctc tgtc
//
LOCUS       DASSZH010000344         5031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1235685, whole genome shotgun sequence.
ACCESSION   DASSZH010000344 DASSZH010000000
VERSION     DASSZH010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235685"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..1270
                     /locus_tag="VFN42_07490"
     CDS             299..1270
                     /locus_tag="VFN42_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET8996495.1"
                     /translation="MMTIRLGVIGAGIMGQRLLHAALDHATDAVQVTGVWDPAEATRA
                     RLAAALPAVPVLDSAAAVIAACDCVYIASPPATHLAHARAGLAAGRAVFCEKPLAVDL
                     AEAREFVAETNGARVAVNFPMASSFGVAQLRDWMAQGTVGTPRTLDIEIAFPTWPRRW
                     QQDAASWLDRPEQGGFTREVLSHFLFLTGRLAGPFALEEAFAAFPEPGRSERAIRARL
                     RAGTLPVTVLGSVGTTRKEDHNTWTLQGPKGAIRLRDWAVAERLGEDGRWQAAPDAVP
                     NEVSRPLVLRHQLLAVSAMTRGEPHPLATVAEALAVQEAVEAILAAR"
     gene            complement(1367..3025)
                     /gene="boxC"
                     /locus_tag="VFN42_07495"
     CDS             complement(1367..3025)
                     /gene="boxC"
                     /locus_tag="VFN42_07495"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HET8996496.1"
                     /translation="MAQSDPIDYRTEPQRYRHWTLSADAPIATLTAAFDEDAGLRPGY
                     KLKLNSYDLGVDIELHDAVNRIRFEHPTVRTVVITSARDKVFCSGANIYMLGVSSHAW
                     KVNFCKFTNETRNGLEDSSQHDGLKFLAAVNGACAGGGYELALACDAILLIDDRSSAV
                     SLPEVPLLGVLPGTGGLTRVTDKRHVRHDLADVFCTTSEGVRGQRAKDWRLVDDIARP
                     AQFAEKVRETALHLAEASIRPASAQGIALTPLLRADEPDALRYRHVAVTIDRAARTAT
                     FTVSGPSGALPGEPAAIQAAGDTWYPLALARELDDAILWMRTNALEIGTWLLKTRGDP
                     AAVLAMDAILLANKDFWLVRETIGYLRRTFSRLDVSSRSLFALIEEGSCFAGTLLELA
                     LACDRSYHLALPDDADRAPKVAVSAANFALYPMATGQTRLQRRFYGEPSPLQAVQGKT
                     GQLLDAEAALALGLVTSSPDDIDWPDETRLAIEERVSMNPDALTGMEASLRFNGKETM
                     LTRIFGRLSAWQNWIFQRPNAVGEKGALKIYGTGARPDFDWDRV"
     gene            3188..3703
                     /locus_tag="VFN42_07500"
     CDS             3188..3703
                     /locus_tag="VFN42_07500"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET8996497.1"
                     /translation="MPPPKITPEQLSPLPEIHRTTVPPAYEDRNGHMNVRWYLVLYDE
                     AGDAMYPMLGLTDAYFASSGMGGFDLEHHLWYLSEVRIGDTVSIRLRMLARNAKLCHY
                     MMFMVNETSGKVSSLFECVHAHADLKARRTAAFPPQAADAIDALIAAQETLAWPAPVS
                     GSMAIKPAPRA"
     gene            3846..3922
                     /locus_tag="VFN42_07505"
     tRNA            3846..3922
                     /locus_tag="VFN42_07505"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3880..3882,aa:Pro,seq:cgg)
     gene            4021..>5031
                     /locus_tag="VFN42_07510"
     CDS             4021..>5031
                     /locus_tag="VFN42_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET8996498.1"
                     /translation="MTAPTLAANVMHFAGLLRRAGLPVGPAETLAGQQALLLVDLGRR
                     DEVRTALRSVMVHRREHQEVFDQAFALFWRDPTAAEQAVAMALLEAQKEKKQDRAPPA
                     SRRVAEAFAPPQKQQRRDDEPPVADAVLTVSDQEKLQQMDFEAMSAAEIDAAKREIRR
                     LVLPLDLRRTRRLRPDPLGPATDLRRTIRASLRQGGEILAIARSRKVTRPPPLVVLCD
                     ISGSMARYAQILLHFLHAVTNDRDRVHVFLFGTRLTNITRQLRHRDAEVAFQMVSHLV
                     PDWSGGTRIGEALAEFNRRWARRVLGQGAVVLMITDGLDRDGAVHLAENMDRLHRSCR
                     RLI"
BASE COUNT          776 a         1732 c         1731 g          792 t
ORIGIN      
        1 tcacgggcag ggtttcccga tgaattcgtg ggaacgatta cttcccgatt agctggctgg
       61 ctacagccat ttctgcaaac cgacgaaaaa ggcagtgtca gccggcctcc ggcggttcag
      121 atgccgcgag acaagggagg catgcaccgg gaatcaaccg gcccccttca gcagagtggc
      181 gcataccgtg ccccttgcgc ctggacgaca cggcggcatc ccgctcccct gcccatttgc
      241 tccccagccc tgccacgcct atcagcagtg ccggacggaa cagcggaaag acgtgcgaat
      301 gatgacgatc aggctgggtg ttatcggcgc gggcatcatg gggcagcggc tgctgcacgc
      361 ggcgctcgac cacgcgaccg atgccgtgca ggtcaccggc gtctgggacc cggccgaggc
      421 cacccgcgcg cgcttggccg ccgccctgcc cgccgtgccc gtgctggaca gcgccgcggc
      481 ggtgatcgcc gcctgtgact gcgtctatat cgcctcgccc cccgccaccc acctcgccca
      541 cgcccgcgcc ggtctcgccg ccggacgcgc ggtgttctgc gagaagccgc tggcggtgga
      601 cctggccgag gcacgcgaat tcgtcgccga aaccaacggc gcgcgggtcg cggtgaactt
      661 ccccatggcc tccagtttcg gcgtcgcgca actgcgcgac tggatggcgc aaggcacggt
      721 gggcacgccg cggacgctgg atatcgagat cgccttcccc acctggccgc gccgctggca
      781 gcaggacgcg gcatcctggc tggaccggcc ggaacaaggc ggcttcaccc gcgaggtgct
      841 gtcgcacttc ctgttcctga ccggccgcct cgccggcccg ttcgccttgg aagaggcgtt
      901 cgccgccttt cccgagcccg gccgcagcga gcgcgccatc cgcgcccgcc tgcgcgccgg
      961 cactctgccg gtgaccgtgc tcggctcggt cggcaccacc cggaaggaag accacaacac
     1021 ctggactttg cagggtccga aaggggcaat ccggctgcgc gactgggcgg tggcggaacg
     1081 gctgggtgag gacggccgct ggcaggccgc acccgacgcg gtgccgaacg aggtctcccg
     1141 cccgctggtg ctgcggcacc agctcctggc ggtgtccgcg atgacgcgcg gcgagccgca
     1201 cccgctggcc acggtggccg aagcgctggc ggtgcaggag gcggtggagg caatcctcgc
     1261 cgcgcgctga gcagaattcg ccggcttgac ccacggacgt ctgcggctca cgcggcgcct
     1321 ctcatgcggc acgcaaccgc cacgtccgcc ggtgccgcct ggcgcgtcag acacggtccc
     1381 agtcgaagtc cggccgcgcg cccgtgccgt agatcttcag tgcgcctttt tcgccgaccg
     1441 cgttgggacg ctggaagatc cagttctgcc aggcgctcag ccggccgaat atgcgcgtca
     1501 gcatggtctc cttcccgttg aagcgcagac tggcctccat gccggtcagc gcatccgggt
     1561 tcatcgacac gcgctcctcg atcgccaggc gcgtctcgtc cggccagtcg atgtcgtccg
     1621 gactgctggt gacaaggccg agtgccagcg ccgcctcggc gtccagcaac tgccctgttt
     1681 tgccctgcac ggcttgcagc ggcgacggct cgccgtagaa gcggcgctgc aggcgggttt
     1741 gtccggtcgc catcgggtag agcgcgaaat tggcggcgct gacagcgact ttcggcgccc
     1801 ggtcggcatc gtcgggcagc gccaggtgat aggaccggtc gcaggccagc gccaattcca
     1861 atagcgtgcc ggcgaagcag gaaccctcct cgatcagcgc gaacaggctg cgcgaggaaa
     1921 catccaaccg gctgaacgtg cggcgcagat aaccgatggt ctcgcgcacc agccagaaat
     1981 ccttgttagc cagcagaatc gcgtccatcg ccagcaccgc cgccggatcg ccccgggttt
     2041 tcagcagcca ggtgccgatc tccaatgcgt tggtgcgcat ccacaggatc gcgtcgtcca
     2101 gctcccgcgc cagggccagc ggataccagg tatcgcccgc tgcctggatg gccgccggtt
     2161 cgccgggcag cgcgccggac gggccactga cggtgaaggt cgcggtgcgg gcggcgcgat
     2221 cgatggttac ggccacatgg cggtaacgca gcgcgtcggg ttcgtcggcg cgcagcagcg
     2281 gggtcagcgc gatgccctgg gcagatgctg gccggatgct ggcttcggcc aggtgcagcg
     2341 cggtttcgcg caccttctcg gcgaactgcg ccggcctggc aatgtcgtcc accagccgcc
     2401 agtccttggc gcgctgaccg cgcacgcctt cgctggtggt gcagaagaca tcggccaggt
     2461 cgtggcgcac gtgccgcttg tcggtgacgc gggtcaggcc gccggtgccc ggcagcacgc
     2521 ccagcagcgg cacttcgggc aggctgaccg cgctgctgcg gtcgtcgatc agcaggatcg
     2581 catcgcaggc aagcgccagt tcatagccgc cgccggcgca ggcgccattc accgcagcaa
     2641 ggaatttcag cccgtcatgc tgcgacgaat cctccagccc attgcgcgtc tcgttggtga
     2701 atttgcagaa gttcaccttc catgcgtggc tggacacgcc gagcatgtag atgttggccc
     2761 cggaacagaa caccttgtcg cgcgcgctgg tgatgaccac cgtgcggacg gtgggatgtt
     2821 cgaagcggat gcggttgacc gcgtcgtgca gctcgatatc gacgccgaga tcgtagctgt
     2881 tcagtttcaa tttgtacccg ggccgcaaac cggcatcctc atcgaaggcg gcggtcagcg
     2941 tggcgatcgg ggcatcggcc gagagcgtcc agtggcgata gcgttgcggc tcggtgcgat
     3001 aatcaatggg atcggactgc gccatcctgg gctcctcgcg ctgcgggtcg gcagcccccg
     3061 cttgcgcggg atggaaccac agatgcgcgt ggatgaacac ggaggcgccg cgtgggccgg
     3121 tccgactcga ccggatcgga aatcgctcta aacagcgagg aaacgcccca gaaccggaac
     3181 ccgatgcatg cccccgccca agatcacgcc cgagcaactg tcgcccttgc cggaaatcca
     3241 ccggaccacg gtcccgccag cctatgagga ccgcaacggc cacatgaacg tgcgctggta
     3301 cctcgtgctg tacgacgagg cgggtgacgc gatgtacccg atgctgggac tcacggacgc
     3361 gtatttcgcg tcctccggca tgggcggctt cgatctggag caccacctct ggtatctgtc
     3421 ggaggtgcgc atcggcgaca cggtatcgat ccggctgcgg atgctcgccc gcaacgccaa
     3481 gctctgccac tacatgatgt tcatggtgaa cgagaccagc ggcaaggttt cgtcgctgtt
     3541 cgagtgcgtg catgcccacg ccgacctgaa ggcgcggcgt accgccgcct tcccaccgca
     3601 ggcggcagat gcgatcgatg cgctgatcgc cgcgcaagag acgctagcct ggccggcgcc
     3661 ggtgtccgga tccatggcga tcaagcccgc gccccgggcc taggaatcgc tcaatgcgcc
     3721 gtcgcgggtg gctctggccc cgccgtttcg ctcggggttg cgtcatccgc ggcggcacgc
     3781 ggcgatgcag cgggttcagg ccgcttgcgg ggcagcgggc acaaggctat atgccgcgcc
     3841 tcggccggag tgtagctcag cctggtagag cactgtgttc gggacgcagg ggccggaggt
     3901 tcgaatcctc tcactccgac cagccgaatt cccgggaaac cgctcggttt tccccatccc
     3961 cttgccgcca ttccctctgc cgcccttgcc tgccgggggc tgtggtgctg tacgcggggg
     4021 atgaccgccc ccaccctcgc cgccaatgtc atgcatttcg ccggcctgct gcgccgagcg
     4081 gggttgccgg tcgggccggc ggagacgctg gccgggcagc aggcgctgtt gctggtggat
     4141 ctgggccgcc gtgacgaagt gcgcaccgcg ctgcgcagcg tgatggtgca ccggcgcgag
     4201 caccaggaag tgttcgacca ggccttcgcg ctgttctggc gcgaccccac ggccgccgag
     4261 caggcggtgg caatggcatt gctggaggcg cagaaggaga agaagcagga tcgcgcgccg
     4321 ccggcctccc gccgcgtggc ggaagccttc gcgccgccgc agaagcaaca gcgccgcgac
     4381 gacgaaccgc cggtggccga tgcggtgctg accgtgtccg accaggaaaa gctgcagcag
     4441 atggacttcg aggcgatgag tgccgcggag atcgacgcgg ccaagcggga aatccgccgc
     4501 ctggtgctgc cgctcgacct gcggcgcacg cgccggctgc ggccggatcc gctcggcccg
     4561 gcaacggatc tgcggcgcac catccgcgcc agcctgcgcc agggcggtga gatcctggcc
     4621 atcgcccgca gccgcaaggt gacccggccg ccgccgctgg tggtgctgtg cgacatctcc
     4681 ggatcgatgg cacggtatgc gcagatcctg ctgcatttcc tgcacgcggt gaccaacgac
     4741 cgcgaccgcg tgcacgtgtt cctgttcggc acgcggctga ccaacatcac gcggcagttg
     4801 cggcatcgcg acgcggaagt ggcgttccag atggtgtcgc atctcgtgcc cgactggtcg
     4861 ggcggcacgc gcatcggcga ggcgctggcg gagttcaacc ggcgctgggc gcgccgtgtg
     4921 ctgggtcagg gcgcggtggt gctgatgatc accgacgggc tggaccgcga tggcgcggtg
     4981 cacttggcgg agaacatgga ccgcctgcac cggtcctgcc gccgtctgat t
//
LOCUS       DASSZH010000345         3602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_979786, whole genome shotgun sequence.
ACCESSION   DASSZH010000345 DASSZH010000000
VERSION     DASSZH010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979786"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(402..1169)
                     /locus_tag="VFN42_07515"
     CDS             complement(402..1169)
                     /locus_tag="VFN42_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HET8996499.1"
                     /translation="MAFSPEYRTYPRASAATTAAVLDAGLRAYMLRVYNWMTSGLLLT
                     GIVAYAIAHTSLINAFYPVVQTPMGPVQHASGLAMLSMFAPLAFVLVLSFGVNKLSTT
                     AAQTLYWVFCAAMGASLTNIFLIYTGDSIVRVFFITAATFAGMSLYGYATKSDLSRWG
                     SFLMMGLIGIIIASLVNIFVASSALQFAVSVVGVLVFTGLTAYDTQRIKATYVQYAYS
                     EGVEMAAKRSVYDALSLYLNFINLFILLLQLMGNQRR"
     gene            complement(1284..>3602)
                     /locus_tag="VFN42_07520"
     CDS             complement(1284..>3602)
                     /locus_tag="VFN42_07520"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET8996500.1"
                     /translation="QPAPKALLNWLHARGDQTSQVVRMRSILVAPSGERQLIDLKAVD
                     DTWPLVGKPAIRPARPLAAALAERRGHYGLVAEQVVLDRLRLHRGDLVKLGNATFRIS
                     GVLISEPDQVATPSLLGPRVVIAAAALPRTGLVVPGAITGHTLRVVLSKPAPTQAAIR
                     AIRSAFPDTGWRIRGPGDAAPGTTRFLDQTSLFLTLVGLTSLLVGGIGVANGVHAWLD
                     ARARTIAILRCLGASSGTVFAVCLIQVMALCAVGVIGGVLAGAALSLGGIVLFRDVLP
                     VPPAMGVYPGPLLLAALYGLLTAAAFSLWPLGRAARIPGGALFRDALVPQRSAPSRRL
                     VAGTLALGAGLIALTVAASQDRWFALWFCAAALAGLAAFRLGGLALVRLARRLPRPKQ
                     AWVRLGLANLHRPATPAPLLLLSVGLGLSTLAAVALIQGNVRQAVLDQLPAGAPSFFF
                     IDIQTDQIARFDHLVDSQPGVQSLRQVPSLRARIVAVDGVPADQVKASPATRWALSGD
                     RGLTYAAEAPPGARIVAGQWWSTDYSGPPLVSFDANLARGWGVKVGGTIRVNVLGRDI
                     DLRVANLRDIAWRSLGLNFSMIATPDLLAHAPHTFIATVRLPDDQQGALLRRVTDALP
                     NVTGIRVADVLGAVADLLDQVAAALAATGSLTLAAGALVLVGAVAAGQRRRTQEAVIL
                     KTLGATRSQIRAAWLVEFGVLGVTAGLLAALIGTAASWAVMHYIMRADWVFLPGTLAE
                     ALFGALALMLVLGYAGTAAALRAKPAPMLRNE"
BASE COUNT          615 a         1312 c         1215 g          460 t
ORIGIN      
        1 agcgtgcctt cgacccggaa atgcgggcca cgccggaact caccatgccg gaggaaaccc
       61 cggatgccct gcagcgcatg ctgggctttg tgctgggcga gcagtggccg atgctgccgt
      121 cctgggttcc tgcgcccgcc cacctgcatc gcgtcgccgc ggcgcacccg ccctgaccgc
      181 atgcgccagg gtcatgcctg acgctcggga ccggacaccg gccggccccg ccatgccgat
      241 tcgcgcgccg tctcgatagc gcccatccgg gcgccgatac gaaaacgcct cccccgccag
      301 tggcggggga ggcgatttcg gttcagcagc cagtcagccg agcatgccgg ccggcgccgg
      361 ccggacgaag ccaggcgcgc cggcgcgtgt cgcggctggt ctcagcggcg ctggttgccc
      421 atcaactgca gcagcaggat gaacaggttg atgaagttca gatacaggct gagcgcatca
      481 tacacgctgc gcttggccgc catctccacg ccctcggaat aggcatactg cacgtaggtc
      541 gccttgatcc gctgcgtgtc gtaggcggtc agcccggtga acaccagcac gccgaccacg
      601 ctcaccgcga actgcagggc agagctggcg acgaagatgt tcaccaggct ggcgatgatg
      661 atgccgatca ggcccatcat caggaagctg ccccagcggc tgagatcgct cttcgtggcg
      721 tagccataca ggctcatgcc ggcgaaggtc gccgcagtga tgaagaacac acggacgatc
      781 gagtcgccgg tgtagatcag gaagatgttg gtcaggctgg cgcccatcgc ggcgcaaaac
      841 acccagtaca gcgtctgtgc cgcggtggtg gacagcttgt tcaccccgaa gctcagcacc
      901 agcacgaagg caagcggcgc gaacatcgac agcatcgcca ggccggaagc gtgctgcacc
      961 ggccccatcg gggtctgcac caccggatag aacgcgttga tcaggctggt atgcgcgatc
     1021 gcataggcga cgatgccggt cagcagcagg ccggacgtca tccagttgta gacccggagc
     1081 atgtaggcgc gcagccccgc gtccaggacg gcagccgtcg tggcggcgga tgcgcgcggg
     1141 taggtccggt actcaggact gaaggccatg gttcccttta cctctcgggc agttgtgccc
     1201 ttgttgacac ccgtggaatc tcggcgatta acccgggcgg ttcaagagcg gaatggtgat
     1261 cccaatcaga cgttgcttgt tcttcattca ttgcgcagca tcggcgcggg tttcgcccgc
     1321 agcgcggctg cggtgccagc atagcccaga accagcatca aggcgagcgc cccgaacagc
     1381 gcctcggcca acgtgccggg caggaacacc cagtccgccc gcatgatata gtgcatcacc
     1441 gcccagcttg cagccgtccc gatcagcgcc gcgagcaggc cggccgtcac ccccagcacc
     1501 ccgaattcca ccagccaggc ggcgcggatc tgcgagcggg tcgcgcccag ggtcttcagg
     1561 atcaccgcct cctgcgtccg ccgccgctgg ccggccgcca ccgcccccac cagcaccagg
     1621 gcgccggcgg ccagggtcag cgaccccgtg gcggccaggg ccgcagccac ctggtccagc
     1681 agatcggcca ccgcgcccag cacatccgcc acccggatgc ccgtgacgtt cggcagcgcg
     1741 tccgtcacgc ggcgcagcag cgccccctgc tggtcgtctg gcagccggac cgtggcgatg
     1801 aaggtgtgcg gtgcgtgtgc cagcaggtcc ggggtggcga tcatgctgaa attcagcccc
     1861 aggctccgcc aggcaatgtc ccgcagattg gcgacccgca ggtcgatatc gcggcccagc
     1921 acgttgaccc ggatcgtgcc gccgaccttc acgccccagc cccgcgccag attggcatcg
     1981 aacgagacca gcggcggacc ggagtagtcc gtggaccacc actgcccggc gacgatgcgg
     2041 gcaccgggcg gcgcttcggc cgcgtaggtc agcccgcggt cgccgctcag ggcccagcgc
     2101 gtggccgggc tggctttcac ctgatccgcc ggaaccccgt ccaccgcgac aatgcgcgcg
     2161 cgcagcgacg gcacctggcg cagcgactgc acgccgggct ggctgtccac cagatggtcg
     2221 aagcgggcga tctggtcggt ctggatatcg atgaagaaga agctgggcgc gccggccggc
     2281 aactggtcca gcaccgcctg ccggacattg ccctggatca gcgccaccgc cgccagggtg
     2341 gacagtccca gcccgaccga aagcagcagc agcggcgccg gcgtggccgg gcgatgcaga
     2401 ttggccaggc caagccgcac ccaggcttgc tttggccgcg ggaggcgccg cgccagccgc
     2461 accagggcga ggccacccag ccggaacgcc gccaatcccg ccagggcggc ggcgcagaac
     2521 cacagcgcga accagcggtc ctgcgaagcg gcaaccgtca gcgcgatcag cccggcgccc
     2581 agcgccagcg tgccggccac cagccggcgc gacggcgcgc tgcgctgcgg caccagcgcg
     2641 tcccggaaca gcgcgccccc cggaatccgc gccgcgcggc cgagcggcca gagggagaag
     2701 gccgcagcgg tcagcagccc gtagagcgcc gccagcagca gcggtccagg gtagacgccc
     2761 atcgccggcg gcaccggcag cacgtcgcgg aacagcacga tgccacccag cgacagcgcc
     2821 gctccggcca gcacgccgcc gatcaccccc accgcgcaca gcgccatcac ctggatcagg
     2881 cataccgcga agaccgtccc ggacgatgcg ccgaggcagc gcaggatcgc gatggtgcgc
     2941 gcgcgggcat ccagccaggc atgcacgccg ttcgccacgc cgatcccgcc caccagcaac
     3001 gatgtcagcc cgaccagggt gaggaacagg ctggtctggt cgagaaaccg ggtggtgccc
     3061 ggcgccgcgt cgccggggcc gcggatccgc cagccggtgt ccggaaaggc cgaccgaatc
     3121 gcccggatcg ccgcctgtgt cggcgcgggt ttcgacagca ccaccctcag cgtgtgcccg
     3181 gttatggccc ccggcaccac cagcccggtc cgcggcagcg ccgcggcagc gatcaccacg
     3241 cgcgggccaa gcagggaggg ggtggcgacc tggtccggct cgctgatcag cacaccgctg
     3301 atgcggaagg tggcattccc cagcttcacc agatcgccgc gatgcagccg cagccgatcc
     3361 agcaccacct gctcggccac cagcccataa tgcccacgac gctcggccag cgcggcggca
     3421 agcggccggg cggggcggat tgccggcttg ccgaccagcg gccaggtatc gtcgaccgcc
     3481 ttgaggtcta tcagttgccg ttcgccggat ggcgccacca ggatggagcg catccgcacc
     3541 acctgtgagg tctggtcgcc gcgcgcgtgc agccagttca gcagcgcctt gggggcgggt
     3601 tg
//
LOCUS       DASSZH010000346         3247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_332065, whole genome shotgun sequence.
ACCESSION   DASSZH010000346 DASSZH010000000
VERSION     DASSZH010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332065"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /gene="amrS"
                     /locus_tag="VFN42_07525"
     CDS             complement(<1..638)
                     /gene="amrS"
                     /locus_tag="VFN42_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HET8996501.1"
                     /translation="MDSHTDPSVVPARHWHRLEDGRIQCDVCPRFCKLHDGQRGLCFV
                     RARQDDGLVLTTYGRSSGFCIDPIEKKPLNHFLPGTPVFSFGTAGCNLTCKFCQNWDI
                     SKAREQDRLNQEAGPARIAAAARQLGCRSVAFTYNDPVIFLEYAVDTAIACHQAGLKT
                     VAVTAGYITPQARGDFYRHIDAANVDLKGFTEGFYRNLCGGRLADVLDTLVWL"
     gene            726..2171
                     /gene="amrB"
                     /locus_tag="VFN42_07530"
     CDS             726..2171
                     /gene="amrB"
                     /locus_tag="VFN42_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HET8996502.1"
                     /translation="MTDIRSPAVAGTFYPADPGTLRRQVAGFLAEAGSAPADAAIPKA
                     IIGPHAGYIYSGPVAAHAYSRLLTARGTIRRVVLIGPSHYVGFQGLAVDTAQAWDMPN
                     GRVALDTDAIGRLRQLPMVGTLEAAYAREHALEVHVPFLQHVLGDFRLVPIVAGDAPP
                     DAVAAVFDALWGGPETLFVISTDLSHYLDYAACRTADSQTAVAIERMDLDALGPQQAC
                     GAVPTRGMLLAARRRGLRIERLDLRNSGDTAGGRDRVVGYGAWALYEPATAATEADEE
                     RAAVEAVGPALIQIARESILLGLNTGAAPQVVHEDGLAPLLAAPGAAFVTLKRRGMLR
                     GCIGSAMATRPLVVDVAVHAFNAAFRDWRFPRLGAEEMAGLSLSVSVLTPPVPVDFAD
                     EAALLAQLRPGIDGLIIRDPGHAALFLPSVWEEIREPETFLDALKQKAGIADGPLPSG
                     FVAQRFRSIEVKGAMQVSTPTAAAGWRLVPG"
     gene            complement(2179..2847)
                     /locus_tag="VFN42_07535"
     CDS             complement(2179..2847)
                     /locus_tag="VFN42_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HET8996503.1"
                     /translation="MPDTAVTDLKPFTRPQMAARVAQDIPEGWYVNLGIGIPTAVADH
                     VPLDREVIFHSENGVLGMGPAPEKDRIDPWLINAGKQFVTLRQGGSFCHHADSFSMIR
                     GGHLDLCVLGAFQVAENGDIANWATSENDTAPAVGGAMDLAAGAKRLWVMMEHTAKDG
                     SPKLVQRCSYPLTAVGAVKRVYTNLAVIDVTERGFVVRDMAPGVAFDTLQARTDAKLH
                     RADQ"
     gene            complement(2952..>3247)
                     /locus_tag="VFN42_07540"
     CDS             complement(2952..>3247)
                     /locus_tag="VFN42_07540"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET8996504.1"
                     /translation="EIAVPIRSRLVVTTAEAAIDAAIAGLGLTRVLSYQVAAAIAAGT
                     LGVVLQGFELAPSPVSLVHRGQLPLPLKSRAFLDFAAPRLKQRIAEVALPRQA"
BASE COUNT          480 a         1075 c         1163 g          529 t
ORIGIN      
        1 agccagacca gcgtatccag cacgtcggca agccggccgc cgcagaggtt gcgatagaac
       61 ccctcggtga aacccttcag gtccacattg gccgcatcga tatggcgata gaaatcgccg
      121 cgcgcctgtg gggtgatata gccggcggtg acggccacgg ttttcaaccc ggcctggtgg
      181 caggcgatcg cggtatcgac cgcatattcc aggaagatga ccgggtcgtt gtaggtgaac
      241 gccaccgacc ggcagccaag ctgccgcgcc gccgcggcga tccgagccgg ccccgcctct
      301 tgattcagcc tgtcctgctc tcgggctttt gaaatatccc agttctggca gaatttgcag
      361 gtcaggttgc agccggcggt gccgaaggaa aacaccggcg tgccgggcag gaaatgattg
      421 agcggcttct tctcgatcgg gtcgatgcag aagccggagg agcggccgta ggtggtcagc
      481 accagcccgt cgtcctggcg cgcgcgcacg aagcacaggc cgcgctggcc gtcatgcagt
      541 ttgcagaagc gcgggcagac gtcgcactgg atgcggccgt cttccagccg gtgccaatgc
      601 cgggcgggaa ccacgctggg atcggtgtga ctatccattc gcttgcctgg cctccaaatc
      661 ccggaggcga tctcctatgt tgaaatccat tcatgcttcc gcattgccag gcaaaaccag
      721 acgccatgac agacatccgt tcgcccgccg tcgcggggac cttctatccg gccgaccccg
      781 gcacgctgcg ccgacaggtt gccggatttc tggcggaggc gggcagtgcg ccggcggatg
      841 ctgccattcc gaaagccatc atcgggccgc atgccggcta catctattcc ggtccggttg
      901 ccgcccacgc ctattcccgg ctgctgaccg cgcgcggcac gatacggcgc gtcgtgctga
      961 tcgggccgtc gcattatgtg gggttccagg gcctcgccgt cgataccgcg caggcctggg
     1021 acatgccgaa tggccgcgtt gcgctggata ccgacgccat cggccggctg cggcaactgc
     1081 ccatggtcgg cacgctggaa gccgcctatg cgcgcgagca cgcgctggag gtccacgtgc
     1141 cgttcctgca gcacgtgctg ggcgatttcc gtctggtgcc gatcgtggcc ggcgacgcgc
     1201 cgccggatgc ggtggccgcg gtgttcgatg cgctgtgggg cgggccggag acgctgttcg
     1261 tgatctccac cgacctgtcg cactacctgg actacgccgc ctgccgaacc gccgattcgc
     1321 agaccgctgt cgcgatcgag cggatggacc tggatgcgct gggcccccag caggcctgtg
     1381 gcgcggtgcc gacacgcggc atgctgctgg ccgcgcgccg ccgcgggctg cggatcgagc
     1441 ggctggacct gcgcaattcc ggcgacaccg cgggcggccg tgaccgggtg gtgggctacg
     1501 gcgcctgggc gctgtacgaa cccgccacgg ccgccaccga agcggatgag gagcgcgcgg
     1561 cggtggaggc ggtgggaccc gcgctgatcc agatcgcgcg ggagtcgatc ctgctgggcc
     1621 tgaacaccgg cgcggcgccc caggtggtgc acgaggacgg gctggcgccg ctgctcgccg
     1681 caccgggcgc cgccttcgtg acgctgaagc ggcgcggcat gctgcgcggc tgcatcggct
     1741 ccgccatggc gacgcggccg ctggtggtgg acgtggcggt gcatgcgttc aatgcggcgt
     1801 tccgcgactg gcgtttcccg cgtctgggcg cggaggaaat ggccgggctg tcgctgtctg
     1861 tttcggtgct gacgccgccg gtgcccgtgg atttcgcgga tgaggcggcg ctgctggctc
     1921 agttgcggcc ggggatcgac gggctgatca tccgcgatcc cgggcacgcc gcgctgttcc
     1981 tgcccagcgt atgggaggag atccgggaac ctgagacatt cctggacgcg ctgaagcaga
     2041 aggcggggat cgccgatggg ccgctgccat ccggcttcgt cgcgcagcgg ttccgctcga
     2101 tcgaggtgaa gggcgcgatg caggtttcca ccccgaccgc ggcggcaggc tggcggctgg
     2161 tgccgggatg accggcggtt actggtcggc gcggtgcagc ttcgcgtccg tgcgagcctg
     2221 caacgtatcg aatgcgactc ccggcgccat gtcgcgcacc acgaaaccgc gctcggtgac
     2281 gtcgatcacc gcgaggttgg tgtagacgcg cttcaccgcg cccaccgcgg tcagcggata
     2341 actgcagcgc tgcaccagct tgggggagcc gtccttcgcg gtgtgctcca tcatcaccca
     2401 gagccgcttg gcgccggccg cgaggtccat cgcgccgccg accgcggggg cggtgtcgtt
     2461 ctcagacgtg gcccagttgg cgatgtcgcc gttctccgcc acctggaagg cgcccagcac
     2521 gcacaggtcc agatggccgc cgcggatcat ggagaagctg tcggcgtggt gacagaagct
     2581 gccgccctgg cgcagcgtga cgaactgctt gccggcgttg atcagccagg ggtcgatgcg
     2641 gtccttctcc ggcgcgggac ccatgcccag cacgccgttc tcggaatgga agatcacctc
     2701 ccggtccagc ggcacgtgat cggcgacggc ggtggggata ccgatgccca ggttgacgta
     2761 ccagccttcc ggaatgtcct gcgcgacgcg cgcggccatt tgcgggcggg tgaaaggctt
     2821 gaggtcggtg acggcggtat cgggcatgat gcctctctcg ctggccggcg cagggggcgc
     2881 ggacgtcctg cattgtctgt ctgccgcgac cttagcgccg ggtggtgcgg ttggccaatc
     2941 catgcggccg gtcaggcctg ccgcggcaat gcgacctcgg cgatccgctg cttgaggcgc
     3001 ggcgccgcga aatccaggaa cgcacgcgac ttcagcggta acggcaactg cccgcgatgc
     3061 accaggctga ccggcgacgg cgcaagctcg aagccttgca gcaccacgcc aagcgtgccg
     3121 gcggcgatgg cggcggcgac ctggtaggac agcacccggg tcagcccgag tccggcaatg
     3181 gccgcgtcga tcgccgcctc ggcggtggtg acgaccagcc gggagcggat cggcaccgca
     3241 atctcca
//
LOCUS       DASSZH010000347         6951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1388930, whole genome shotgun sequence.
ACCESSION   DASSZH010000347 DASSZH010000000
VERSION     DASSZH010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6951
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388930"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3445)
                     /locus_tag="VFN42_07545"
     CDS             complement(<1..3445)
                     /locus_tag="VFN42_07545"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8996505.1"
                     /translation="MNGAQPNSPGRAGPDTAGPSDAFLQSAGEMASLIAAKDWSATPL
                     GPAEFWPRSLHTVLRILVTSRYAMWLGWGADLIFFYNDAYAEMTLGGKHPWALGQPAS
                     TVWSEIWPDVGPRAESAMRTGKATWDEGLLLFLERSGFPEETYHTFSYSPLPDDEGRI
                     GGMLCVVTEETQSVIGERRLATLRELGAELAAVRTEPEVMTAIERGLVVNARDLPFTA
                     TWLFDPEPGQAHLACVTGIPREHPLLAQTVDLAVLGADDPFRRILNGESHVLIDGWPP
                     DLALPTGAWDRPPRGALAVPISQQGAERPAGMLLAALNPYRPFDEAYQGFIDLVAAQV
                     ASGLASARAYEAERRRAEALAELDRAKTAFFSNISHEFRTPLTLMLGPLEEELIETAG
                     EAAGRLTMVHRNGLRLLRLVNMLLDFSRIEAGRVRASFRPTDLAAYTGELASNFRSAC
                     ERAGLTLDVDCPPLPHPVYVDADMWERVVLNLVSNAFKYTLNGGIRVSVEAAGDRQAQ
                     LVVSDTGVGIPADELPRIFDRFHRIEGQSGRTMEGTGIGLSLVHELVRLHGGQIAVQS
                     QVNAGTTVRVSLPFGHAHLPQDQVSLDRGRSIPEGVAAASFVEEALRWLPDEGGATEM
                     PALLEPATILAGISAGAAADGQRRRILLADDNADMRDYIMRLLGPRYDVHAVEDGAAA
                     LAFMQADRPDLLLSDVMMPRLDGFALLQAIRSDPELRSIPVILLSARAGDEARVEGLD
                     AGADDYLVKPFGARELLARVRANLEMARIRREAAEQVAEEARRLKILNRTGSTIAAEL
                     DLERLVQSVTDAAVELVGAKFGAFFYNVLDETGEAYTLYTLSGASREDFAGFPMPRNT
                     AVFEPTFRGEGIVRSDDILADPRYGRNEPYNGMPSGHLPVRSYLAVPVISRSGEVVGG
                     LFFGHPDPGVFSERAELIVGGIAAQAAVGIDNARLYRASQQAQQGLRQLNETLEQRVA
                     EEIAERMKAEEALRQAQKMEAVGQLTGGVAHDFNNLLTVITGGVEVLQRQFANTAASA
                     DSARVNRALRMIEQGANRAATLTQRLLAFARRQALDPRALDVNKLVSGMSELLRRTLG
                     ETIRIETVQAGGLWRSMADPNQLENALINLAVNARDAMPAGGVLTMRAALAPARPDEA
                     PI"
     gene            3595..4686
                     /locus_tag="VFN42_07550"
     CDS             3595..4686
                     /locus_tag="VFN42_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HET8996506.1"
                     /translation="MKAAVFQAPHQPLSIEEVEIEKPKRREVLLRTAYAGLCHSDLHF
                     MEGLYPFPLPAVLGHEAAAVVEAVGEDVTYVQPGDHVITCLSVFCGECPQCVTGHTNL
                     CENTEVKLLPGAARRLRWKGQVLNQAFNLSAFAENMLVHEHAVVKINRDIPLDRAALV
                     GCGVMTGVGAVFHAAKVEPGSTVAVVGCGGIGLSAVNGAALAGASKIIAIDTNSAKLE
                     KAKEVGATDTINASNVDPVEAVKEMTGGQGVPYSFEAIGLKATAEQCFAMLRPGGVAT
                     IIGMIPFGTKIELHGADFLRDRKIQGTSMGGNRFRVDMPRLLELWQQGRLKLDTLISG
                     KIRLDQINEGFDQLKSGVPLRNLIDFGVV"
     gene            4689..5084
                     /locus_tag="VFN42_07555"
     CDS             4689..5084
                     /locus_tag="VFN42_07555"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET8996507.1"
                     /translation="MPRTAIVSERIGKPSGHFAQAIAVEARGRLVFISGMTSKGADGS
                     VVGVGDLEAQTRQICENVKAAVEAAGGTMADICRVDVYVKDMSGFDVIHRVRREYFPD
                     PPPASTMVQVSGFTHPDYLIEMNAIAVVG"
     gene            5243..6280
                     /locus_tag="VFN42_07560"
     CDS             5243..6280
                     /locus_tag="VFN42_07560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8996508.1"
                     /translation="MRLGVFDHMDRSQAPLDQFFADRLRLVEAYDRAGFYAYHVAEHH
                     ATPLGVAPSPGIWLSAIAQRTTRLRFGALVYLLPFYHPLKLLEELCMLDQMSRGRLLL
                     GVGRGISPIELRYYGLDPEQTPAMYQEALEVILRGMTCKELTFEGKYYNYRDVPMELE
                     PYQKPHPPLWYGIGRTDAVPWAARHNVNVVGNVTGEKMRLLTDAYRAEWARLGHTPET
                     LPLMGLGRHVVVGETEREALDVARRGYHRWRDSFLLLWRKHNTLPANPNVLFPETFEE
                     AEAEGRAVAGPPEKVRRFLQSEIDAGGLNYLLCRFAFGDITADEALHSVDLFARQVMP
                     DLHEARVGIAA"
     gene            6277..>6951
                     /locus_tag="VFN42_07565"
     CDS             6277..>6951
                     /locus_tag="VFN42_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HET8996509.1"
                     /translation="MTAIYEVFAIRYATRAAHRSDHFIGGDPHDGPMPMDYFVWVAKN
                     AERAVVIDIGFTAESGGKRGRALLRDPIDSLALVGVDAAQVRDVVLTHLHYDHVGNFH
                     KFPVARFHLQEPEIHYAVGRHMRHKQLAKSFEPDDVCGIVRLNFDGRVTYYNGAAAIV
                     PGIGVVPTGGHSAGLQFVTVNTARGVVVLASDVTHFYENMEAGRPFTTAFHIGDMLDA
                     FDTLRAH"
BASE COUNT         1156 a         2288 c         2407 g         1100 t
ORIGIN      
        1 aaatgggcgc ctcgtccggc cgcgccgggg cgagggctgc gcgcatcgtg agcacgccgc
       61 ccgcgggcat ggcgtcgcgc gcgttgacgg cgaggttgat cagggcgttc tccaactggt
      121 tggggtcggc catgctgcgc cagagcccgc cggcctgcac cgtctcgatg cgaatcgtct
      181 cgcccagcgt gcggcgcagc agctcggaca tgccggagac cagcttgttc acgtccagcg
      241 cacgcggatc caatgcctgg cgccgcgcga aggccagcaa acgctgggta agcgtggccg
      301 cacggttggc accctgctcg atcatgcgca gggcgcggtt gacgcgtgcc gaatcggcgg
      361 aggcagccgt attggcgaac tgccgttgca gcacctccac cccgccggtg atcaccgtca
      421 gcaggttgtt gaaatcgtgc gcgacgccgc cggtcaactg cccgaccgct tccatcttct
      481 gcgcctggcg gagcgcctcc tcggccttca tccgctcggc gatctcttcc gcgacgcgct
      541 gctccagcgt ctcgttgagc tgccgcagcc cttgctgcgc ctgctggctg gcccggtaca
      601 gccgcgcatt gtcgatgccg accgcggcct gcgcggcgat gccgccaacg atcagctcgg
      661 cgcgttcgct gaacacgccg ggatcgggat ggccaaagaa caggccgccg accacctcgc
      721 cggaccgcga gatcaccggc acggcgaggt agctgcgcac tggcagatga ccggacggca
      781 tgccgttgta cggctcgttc cgtccatacc gcggatcggc aaggatatcg tcggagcgca
      841 cgataccctc gccgcggaag gtcggttcga acaccgccgt attgcgcggc atcggaaagc
      901 cggcgaagtc ttcccgcgaa gctccggaca gggtgtacag ggtgtaggct tcgccagtct
      961 cgtccagtac attgtagaag aaggcgccga atttggcccc gaccagttcg accgctgcat
     1021 cggtgacgct ctgcaccagc cgttccagat cgagttccgc ggcaatcgtg ctgccggtcc
     1081 ggttcaggat cttcagccgg cgtgcttcct ccgccacctg ctccgccgcc tcgcggcgga
     1141 tgcgcgccat ttccagattg gcgcgcaccc gcgccagcaa ctcgcgcgcc ccgaacggct
     1201 tgaccaggta gtcgtccgcg ccggcatcca ggccttccac ccgcgcttcg tcgccggctc
     1261 gtgcggacag caggatcacc gggatgctgc gcagctccgg atcgctgcgg atggcctgga
     1321 gcagggcgaa cccatccagc cgcggcatca tcacatcgga cagcagcagg tccggccggt
     1381 ccgcctgcat gaaggccagc gccgcggcac cgtcctccac cgcgtgcacg tcatagcgcg
     1441 ggccaagcag ccgcatgatg tagtcgcgca tatcagcatt gtcgtccgcc agcaggatgc
     1501 ggcggcgctg gccgtccgcg gcggcgcccg cgctgatgcc ggcaaggatc gtggcaggct
     1561 ccagcagtgc cggcatctcg gttgccccgc cctcatccgg caaccagcgc agcgcctcct
     1621 ccacgaagga tgcggccgcg accccttccg ggatgctgcg cccgcgatcg agggagacct
     1681 ggtcctgcgg cagatgcgcg tggccgaacg gcaggctgac cctcaccgtg gtgccagcat
     1741 tcacctggct ctgcacggcg atctgcccgc catgcagccg caccagctca tgcaccaggg
     1801 acaggccgat gccggtgcct tccatggtgc ggccggactg gccctcgatg cgatggaagc
     1861 ggtcgaagat acgcggcagc tcgtccgccg ggatgcccac accggtatcg ctgacgacga
     1921 gctgtgcctg ccgatcgcct gcggcttcta cgctgacgcg gatgccgccg ttcagcgtgt
     1981 atttgaacgc gttggacaca aggttcagca ccacgcgttc ccacatatcc gcatcgacat
     2041 agacgggatg cggcagaggc gggcagtcaa cgtccaatgt caggccggcg cgttcacagg
     2101 cagagcggaa gttgctggcg agttcgccgg tgtacgcggc gagatcggtc ggccggaagc
     2161 tggcgcgcac gcggccggct tcgatacggg agaagtccag cagcatgttg accagccgca
     2221 gcaggcgcag gccgttgcgg tgcaccatgg tcagccggcc ggcagcttcg ccggcggttt
     2281 cgatgagttc ctcctccagg gggcccagca tcagggtgag cggcgtgcgg aactcgtggc
     2341 tgatattgga gaagaaggcg gtcttggcgc ggtcgagttc ggccagcgcc tcggcccgcc
     2401 gacgttcggc ttcataggcg cgggcactgg cgagcccgga cgccacctgc gccgcgacca
     2461 ggtcgatgaa gccttgatac gcctcatcga acgggcggta tggattgagt gcggccagca
     2521 gcatgccggc cggccgctcg gcgccctgct ggctgatcgg caccgccagc gcgccgcgcg
     2581 gcgggcggtc ccaggcgccg gtgggaagcg ccagatccgg cggccagcca tcgatcagca
     2641 cgtgcgactc gccgttgaga atgcggcgaa acggatcatc cgcgccgagc acggcaagat
     2701 cgacggtctg ggcgagaagc ggatgctcac gcgggatacc ggtgacgcag gcgagatgcg
     2761 cctggcccgg ctccggatcg aacagccagg tggcggtgaa gggcaggtca cgggcgttga
     2821 ccacaaggcc acgctcgatc gcggtcatga cctccggttc ggtgcggact gcggcaagtt
     2881 ccgcaccaag ctcccgcaag gtggccagcc ggcgctcgcc gatgacgctt tgcgtttctt
     2941 cggtgacgac gcacagcatg ccgccgatcc ggccctcatc gtccggcagc gggctatagg
     3001 aaaaggtgtg gtaggtttcc tccggaaagc cgctgcgttc caggaacagc agcagcccct
     3061 cgtcccaggt ggccttgccg gtgcgcatgg cggattcggc acgcggaccg acgtccggcc
     3121 agatctccga ccagacggtc gaggcaggct gtcccagcgc ccacgggtgc ttgccgccga
     3181 gcgtcatttc ggcgtacgca tcgttgtaga aaaagatcag atccgctccc cagccaagcc
     3241 acatcgcgta gcgcgacgtc accaggatgc gcaggacagt gtgcaggctg cgcggccaga
     3301 attcagccgg accgagcggg gtggcggacc agtccttggc cgcgatcaga gaggccattt
     3361 cgccagccga ttgcaggaag gcgtcggacg gcccggcggt atccggccca gcccggcccg
     3421 gcgaattcgg ctgcgcgccg ttcattcgtg aaactcatcg aagtgctgca agcgtttgca
     3481 ggtggcacgc ctgccgcaac gagtcgactc acgtaatgtt gagccgcggc gggccggctt
     3541 cctttctccc cggccgctgc tagactcgga ccgacaagaa ccaaggggga cgccatgaag
     3601 gccgccgtgt tccaggctcc gcaccagccg ctcagcatcg aggaggtgga gatcgagaag
     3661 ccgaagcggc gggaagtgct gttgcgcacg gcctatgccg ggctgtgcca ttccgacctg
     3721 cacttcatgg agggactgta tccgtttccg ctgccggcgg tgctgggcca cgaggccgcg
     3781 gcggtggtgg aggcggttgg cgaagatgtt acttacgtgc aaccgggcga ccacgtcatt
     3841 acctgcctgt cggtgttctg cggcgagtgc ccgcaatgcg tcaccggcca caccaacctc
     3901 tgcgagaaca ccgaggtgaa gctgctgccc ggagctgcgc ggcggctgcg ctggaaggga
     3961 caggtgctga accaggcgtt caacctgtcc gccttcgccg agaatatgct ggtgcacgag
     4021 catgcggtgg tgaagatcaa ccgcgacatc ccgctggatc gcgccgccct ggtgggctgt
     4081 ggcgtgatga ccggggtcgg cgcggtgttc catgcggcga aggtggaacc cggcagcacc
     4141 gtggcggtgg tgggctgcgg cggcatcggc ctgtcggcgg tgaacggcgc ggcgctggcc
     4201 ggcgcgtcca agatcatcgc catcgacacc aactccgcca agctggagaa ggcgaaggag
     4261 gtcggcgcca ccgacacgat caacgcgtcc aacgtcgacc cggtggaagc ggtgaaggaa
     4321 atgaccggcg gccagggcgt cccctactcg ttcgaggcga tcggcttgaa ggcgacggcg
     4381 gagcagtgct tcgccatgct ccgtccgggc ggcgtggcga cgatcatcgg catgatcccg
     4441 ttcggcacga agatcgagct gcacggcgcg gacttcctgc gcgaccgcaa gatccagggc
     4501 acgtcgatgg gcggcaaccg gttccgcgtg gatatgccgc ggctgctgga gctgtggcag
     4561 cagggacggc tgaagctgga cacgctgatc tccggcaaga tcaggctgga tcagatcaac
     4621 gagggcttcg accagctgaa gtccggcgtg ccgctccgca acctgatcga tttcggggtg
     4681 gtctgagcat gccgcgcacc gcgatcgttt ccgagcgcat cggcaagccg agcggccact
     4741 tcgcacaggc gatcgcggtg gaggcgcgcg gccggctggt atttatctcc ggcatgacat
     4801 cgaagggcgc ggacggcagc gtggtcggtg tcggcgacct ggaagcgcag acgcggcaga
     4861 tatgcgagaa cgtcaaggcg gcggtggaag cggccggcgg cacgatggcg gatatctgcc
     4921 gggtggatgt gtacgtgaag gacatgtccg gcttcgacgt gatccatcgc gtgcggcgcg
     4981 agtacttccc cgatccgccg ccggcatcga cgatggttca ggtcagcggg tttacgcatc
     5041 cggactacct gatcgagatg aacgcgatcg ctgtggtggg atagcgcgcc agggcaaagg
     5101 actccccctc cccttgcggg agggggctgg ggggaggggg cgatcgaggt tgcccggcca
     5161 acgcaggcac ccccctcccc ccaacccctc ccgcaaggaa aggggggttc tttgtccagc
     5221 gcagcaaggg caggaaacga tgatgcgact cggcgtattc gaccacatgg accgcagcca
     5281 ggcgccgctg gaccagttct ttgccgaccg ccttcgcctg gtggaagcgt atgaccgcgc
     5341 cggcttctac gcctaccatg tggccgaaca tcacgcgacg ccgctgggcg tggcgccgtc
     5401 gcccggcatc tggctgtcgg cgatcgccca gcgcaccacg cggctgcgct tcggcgcgct
     5461 ggtgtatctg ctgccgttct accaccccct gaagctgctg gaagaactgt gcatgctcga
     5521 ccagatgagc cgcggccgcc tgctgctggg cgtcggccgc ggcatctcgc cgatcgagct
     5581 gcgctattac gggctggatc ccgagcagac gccggcgatg taccaggaag cgctggaggt
     5641 gatcctgcgt ggcatgacct gcaaggaact caccttcgaa ggcaaatact acaactatcg
     5701 cgacgtgccg atggagctgg agccatacca gaagccgcat ccgccgctgt ggtacggcat
     5761 cggccgcacc gacgccgtac cctgggcggc tcggcacaac gtgaacgtgg tcggcaacgt
     5821 caccggcgag aagatgcgcc tgctgaccga cgcgtatcgc gccgagtggg cacgcctcgg
     5881 tcacacgccg gagacgctgc ctctgatggg gctgggccgc cacgtcgtgg tgggggaaac
     5941 cgaacgggag gcgctggacg tcgcccgccg cggctaccac aggtggcggg acagcttcct
     6001 gctgctgtgg cgcaagcaca atacgctgcc ggccaatccg aacgtgctgt ttcccgagac
     6061 attcgaggaa gccgaagcgg aaggccgcgc ggtggccggg ccgccggaga aggtgcgccg
     6121 cttcctgcag tccgagatcg atgccggcgg gctgaactac ctgctgtgcc gcttcgcctt
     6181 cggcgacatc acggcggatg aggcactgca ttccgtcgac ctgttcgccc ggcaggtgat
     6241 gccggacctg catgaagccc gcgtggggat tgccgcatga ccgcgatcta cgaagtcttc
     6301 gccatccgct acgcgacgcg tgcggcgcac cggtcggacc acttcatcgg cggcgacccg
     6361 catgacgggc cgatgccgat ggactacttc gtctgggtgg cgaagaacgc cgagcgcgcc
     6421 gtggtgatcg atatcggctt caccgcggaa agcggcggca agcgcgggcg cgcgctgctg
     6481 cgcgacccga tcgactcgct ggcgctggtg ggggtggatg cggcgcaggt gcgggacgtg
     6541 gtcctgacgc acctgcacta cgaccacgtc ggcaatttcc acaaattccc cgtggcgcgg
     6601 tttcatctgc aggagccgga gatccactat gcggtcgggc ggcacatgcg ccacaagcag
     6661 ctggcgaaat cgttcgagcc ggacgacgtg tgcggcatcg tgcggctgaa cttcgacggg
     6721 cgggtgacat actataacgg cgcggcggca atcgtgccgg gcatcggcgt ggtaccgacc
     6781 ggcggccact cggcggggct gcagttcgtc acggtgaaca ccgcccgcgg cgtggtggtg
     6841 ctggcctccg acgtgaccca tttctacgag aacatggaag ccggccggcc gttcaccacg
     6901 gcgttccata tcggcgacat gctggacgcg ttcgacaccc tgcgcgcgca t
//
LOCUS       DASSZH010000348         2559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1508598, whole genome shotgun sequence.
ACCESSION   DASSZH010000348 DASSZH010000000
VERSION     DASSZH010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508598"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VFN42_07570"
     CDS             complement(<1..619)
                     /locus_tag="VFN42_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996510.1"
                     /translation="MLITGTVSGTAVLPVPYNIVVDDATTPVTVVANGTSPQVASVTR
                     IGQLYGAQGGAGYFISGNFSAQGSASMGTGGGSGGGNLWVLGAVGNGNSFLASDSRAD
                     SIVAAQGANWIATGLNSSDRIWINGGTAVIASRGADTIAIGAAEATVVGSGEALVFAG
                     SGKAVVAAIAGTDSVIGSTAAVTVTGSDSASLLAYGGSAGGNVMLN"
     gene            1157..2119
                     /locus_tag="VFN42_07575"
     CDS             1157..2119
                     /locus_tag="VFN42_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HET8996511.1"
                     /translation="MSRAQQQFKPIPTTPPELPGNPASAAKIELGKMLYFEPRLSASH
                     TISCNSCHNIGLGGADAESTSLGHRWQHGGRNAPTVFNAVFNTAQFWDGRAKDLEQQA
                     GGPMVNPVEMASPTPHVAEQLKGIPGYANAFAKAFPGESDPISLANVQKAIAVFEATL
                     ITPDAPFDRFLKGDASALSPTQQAGLALFMDKGCSSCHNGINVGGGMYAPFGVVEKPG
                     ASLLPPGDKGRFMVTKTPSDEYVFKVPTLRNIALTAPYFHTGQAWDLGQAVAVMGTSQ
                     LGTQLTADEIGKITDFLGSLTGTQPNVTYPILPPSVATTPRPQP"
     gene            complement(2293..2559)
                     /locus_tag="VFN42_07580"
                     /pseudo
     CDS             complement(2293..2559)
                     /locus_tag="VFN42_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
BASE COUNT          450 a          890 c          808 g          411 t
ORIGIN      
        1 tgttgagcat cacgttgccg cccgcgctgc cgccataggc cagcaggctg gcgctgtccg
       61 atccggtcac cgtcaccgcc gccgtgctgc cgatcacgct gtccgtaccg gcgatggccg
      121 cgacgaccgc cttgccgctg ccggcgaaaa ccagcgcctc gccgctaccg accaccgtag
      181 cctccgccgc gccgatcgcg atcgtgtcag caccgcggct tgcgatgacg gctgtgccgc
      241 cgttgatcca gatccggtcg ctgctgttga ggccggtcgc tatccagttg gcgccctgcg
      301 cggccacgat gctgtcggcg cggctatcgc tggcgaggaa actgttgccg ttgccgacgg
      361 cgccgagcac ccagagattg ccaccgccgc tgccaccgcc ggtacccatg ctcgcgctgc
      421 cctgggcgct gaaattgccg gagatgaagt agccggcacc accctgcgcc ccgtagagct
      481 gcccgattct ggtgacgctt gcgacctgcg ggctggtgcc attcgccacc acggtgaccg
      541 gcgtcgtcgc gtcgtcaacc acgatattgt agggcacggg aagcaccgcc gtgccgctca
      601 ccgtgccggt gatcagcacc gatccgagtt ggccggaggg gaccgtcgga aaggttccgc
      661 ccggcaccca gacatagggt gcgcccggcg cgctggcaga caccgccgct accccgaagg
      721 cgatgaccga atcgatcgcc gcggcgagcg ggttcgtcgc gttggggccg cccggtccac
      781 ccgggccggc gttccccgga gccgatccac cgccgctgcc cgacatgcac ggttctcctt
      841 atagcaatta atggaatccc gtagcagtgt ttgcggcggc ggacggagca ggcttgatgc
      901 agatcaaccg aatgatcacg atcgcgcggc gtgcgacacg caatcgtgcc cgacggaaac
      961 ggtcaacttc cataaatgcg ctgacgcagg tcaacgtcgc ccgcgtggcg ccgccggatg
     1021 aacggtgccg ccacgcccgc ggcgcggctg cgtcgcgaaa acagcaagga agatcgacat
     1081 ggtcaggaac gccatcctcg ctctgctgcc cgtcggttgc ctgctatcaa cacccgctct
     1141 cgcagaaggc gatctgatga gccgggcaca gcagcaattc aagccgatcc ccaccacgcc
     1201 gccggaactg ccgggcaatc ccgcctctgc ggccaagatc gaacttggca agatgctcta
     1261 tttcgagcca cggctgtcgg cgagccacac gatcagctgc aattcctgcc acaatatcgg
     1321 cctcggcggc gccgacgcgg agtccacctc gctcggccat cgctggcagc atggcggacg
     1381 caacgcgccg accgtgttca acgcggtatt caacacggcg cagttctggg acggacgcgc
     1441 caaggacctt gaacaacagg ccggcggccc gatggtgaac ccggtcgaaa tggcgtcgcc
     1501 cacgccccat gtcgccgaac agttgaaggg aatccccggc tatgcgaacg cttttgcaaa
     1561 ggcgtttccc ggagaatccg atcccatctc gctcgccaat gtgcagaagg cgatcgccgt
     1621 gttcgaggcg acgctgatca cgccggatgc tcccttcgac cgcttcctga agggcgacgc
     1681 ctcggccctg tcgccgacac agcaggcggg gctggcgctg ttcatggaca aaggctgttc
     1741 aagttgccac aacggcatca atgtcggcgg gggcatgtat gcgccgttcg gcgtggtgga
     1801 aaagcccggc gccagcctgc tgccgcccgg tgacaagggc cgcttcatgg tcacgaagac
     1861 gccgagcgac gaatacgtct tcaaggtgcc gacattgcgc aacatcgcgc tgacggcacc
     1921 gtatttccat accggacagg cctgggacct cggacaggcc gtcgccgtca tgggcacgag
     1981 ccagcttggt acgcagctca ccgccgacga gatcgggaag attaccgact tcctgggcag
     2041 cctgaccggc acgcagccga atgtcacgta tcccatcctg ccaccgagcg ttgccaccac
     2101 gccgcgcccg cagccgtaaa catgatgcca tcctccaggc ccagcctgga gggtggccca
     2161 gcgcccagtg gcagatgcgc accacgcgga cccgcggcgc ataacggtga gcgacgcccg
     2221 gctgctttga tctcggtcag ccggggcgag cgggtgctgc acgccctggt tcatgccgat
     2281 ggggcggacg aacaccacgc gttccttcgc atattgcgcg gcgcggcgca gcgcgtcccg
     2341 cccgacgccg atcgcctcgc tgccgaccag aacgcgctcg ggattgagac tgtcgaggat
     2401 gtagtgaaat cccctgccct cctcgccgat gcggtcggct tcggggatga acaggcagtc
     2461 tatgaacacg gcattcgagt cgattgcgtt gcggcccacc ttgcgaatgc gccgcacctc
     2521 gatcttcgcg cggtcgaggt cggtgtagaa cagcgtcat
//
LOCUS       DASSZH010000349         2401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1474257, whole genome shotgun sequence.
ACCESSION   DASSZH010000349 DASSZH010000000
VERSION     DASSZH010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474257"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..559
                     /locus_tag="VFN42_07585"
     CDS             98..559
                     /locus_tag="VFN42_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996512.1"
                     /translation="MVPSANHTEPDPIAAGPGRQTAESNREIAAHQQAEAETDRAHAE
                     QERIVAEESRNTAERLRELSETTRAAAEEVRAGREETRQAREEIRALAEEVRRLAEQS
                     RAEAERLRAEAEQFRAEAEAGRSALAQQRQVFEEMLRSTWPGARPGADAGD"
     gene            667..1344
                     /locus_tag="VFN42_07590"
     CDS             667..1344
                     /locus_tag="VFN42_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HET8996513.1"
                     /translation="MANVVTIDAEARARAGKGAARATRRSGKVPAVIYGGHQAPTLVA
                     LDPRDVIRETSRAGWQSRQYEIKTNGDATRALNRDVQYHPVSDAPQHVDFQRLAPGER
                     IRVAVPVHFENEGISPGLKRGGVLNVVRHAVEVYCDPDQIPEQFVADLGSLDFNDNVR
                     WHDLRGTENAKPVIDRDFVVATVAPPTKAAETAEATTAATPAAAAPAARAAAPAKAPA
                     KAPAKKG"
     gene            1367..1945
                     /gene="pth"
                     /locus_tag="VFN42_07595"
     CDS             1367..1945
                     /gene="pth"
                     /locus_tag="VFN42_07595"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET8996514.1"
                     /translation="MLLWVGLGNPEPGMARNRHNIGFMALDTIAIRHGFSPWRQRFKG
                     VVCEGNLAGEKIVALKPQTYMNASGDSVQAAAAFYKLPLELITAFHDELDLVPGKVRV
                     KRGGGAAGHNGLRSMDRMLGSPDYWRVRLGIGHPGDKARVLGHVLGDFAAADREWLLP
                     MLDAVADAAPLLVQGKPEDFMTRVAYLVQEPK"
     gene            1946..>2401
                     /locus_tag="VFN42_07600"
     CDS             1946..>2401
                     /locus_tag="VFN42_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019086085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HET8996515.1"
                     /translation="MGFNCGIVGLPNVGKSTLFNALTATAAAQAANYPFCTIEPNVGR
                     VAVPDKRLDVLAAIGKSAKIIPTSLEFVDIAGLVRGASKGEGLGNQFLANIREVDAII
                     HVLRCFEDPDITHVEGPIDPIRDAEIVETELMLADLESLEKRLNTAQKRA"
BASE COUNT          435 a          812 c          842 g          312 t
ORIGIN      
        1 gtgacaccag tgcgaatccg gcggatagtt cagccgatgg ccgcgtccgg cacgccaccg
       61 gcgcaggcac gcgcccgcgt gaaaaggaca cctgaccatg gtgccatccg cgaaccacac
      121 cgagccggac ccgattgccg ccggaccagg ccggcaaacg gcggaaagca atcgggaaat
      181 cgccgcacac cagcaggccg aggccgaaac ggaccgcgcg cacgcagagc aggaacgcat
      241 cgtcgccgag gaatcgcgca acaccgccga acgcctgcgc gaactgagtg aaaccacgcg
      301 tgccgcggcg gaggaggtgc gcgccggacg ggaggagacg cgtcaggcca gggaggaaat
      361 ccgcgccctg gccgaggaag tccgccgcct ggcggaacaa agccgcgccg aagcggaacg
      421 tctccgcgcg gaggccgaac agtttcgcgc cgaagccgaa gccggccgct ccgcgctcgc
      481 gcagcagcgg caggtgttcg aggagatgtt gcggagcacc tggcccgggg cgcgccccgg
      541 cgccgatgcc ggtgactgac ctgcgactga cccggcgcgc ttgccccctg ccccgccctc
      601 cgctacatcc ccggcctgcg ccggcacccc tggaggccgg cttttgctat ttgtcaggag
      661 cgaagcatgg ccaatgtcgt gaccatcgac gccgaggccc gcgcgcgagc cggtaagggg
      721 gcggcgcgtg caacccggcg gtcgggcaag gtgcccgcag tgatctacgg aggccaccag
      781 gcccccaccc tcgtcgcact cgacccgcgc gacgtgatcc gggagaccag ccgagccggc
      841 tggcagtccc ggcagtacga gatcaagacc aacggcgacg ccacccgcgc gctgaaccgt
      901 gacgtgcagt accatccggt cagcgatgcg ccgcagcatg tggacttcca gcgcctcgcc
      961 ccgggcgagc gcatccgcgt ggcggtgccg gtgcacttcg agaacgaggg catcagcccc
     1021 ggcctgaagc gcggcggcgt gttgaacgtg gtgcgccacg cggtggaggt gtactgcgac
     1081 ccggaccaga ttccggagca gttcgtcgcc gacctgggca gcctggattt caacgacaac
     1141 gtccgctggc acgacctgag gggcaccgag aacgccaagc cggtgatcga ccgcgacttc
     1201 gtggtcgcca cggtggcgcc gccgaccaag gcggcggaaa ccgcggaagc caccacggca
     1261 gcgactccgg ccgccgccgc accggccgcg cgcgccgccg cgccggccaa ggctccggcc
     1321 aaggcaccag ccaagaaggg ctgatcccgg caagggaccg catgacatgc tgctctgggt
     1381 cggactcggc aatccggagc cgggaatggc ccggaaccgg cataacatcg gcttcatggc
     1441 gctggacacc atcgcgatcc gccacgggtt ctccccgtgg cggcagcgat tcaagggcgt
     1501 ggtgtgtgag ggcaaccttg ccggcgagaa gatcgtcgcg ctgaagccgc aaacctatat
     1561 gaacgcctcc ggtgactcgg tacaggccgc ggcggcgttc tacaaactgc cgctggagct
     1621 gatcaccgcg ttccacgacg agctggacct ggtgcctggc aaggtgcggg taaagcgcgg
     1681 cggcggtgcg gccggccata acgggctgcg cagcatggac cgcatgcttg gctcgccgga
     1741 ttactggcgc gtgcgcctgg gcatcggcca tcccggcgac aaggcccgcg tgctcggcca
     1801 cgtgctgggc gatttcgccg ccgccgatcg ggagtggttg ctgccgatgc tggacgcggt
     1861 ggcggatgcg gcgccgctgc tggtgcaggg caagccggag gatttcatga cgcgcgtcgc
     1921 gtatctggtg caggaaccga aatagatggg tttcaactgc ggcattgtcg gcctgccgaa
     1981 tgtgggcaag tccacgctgt tcaatgcgct gaccgccacc gcggcggcgc aggcggcgaa
     2041 ttatccgttc tgcacaatcg aaccgaatgt cggccgcgtc gccgtgccgg acaagcggct
     2101 ggacgtgctg gcggcgatcg gcaaatcggc gaagatcatc ccgacgtcgc tggagttcgt
     2161 cgacatcgcc ggcctggtgc gcggtgcgag caagggcgaa ggcctgggca accagttcct
     2221 cgccaatatc cgcgaggtgg acgcgatcat ccacgtgctg cgctgcttcg aagatcccga
     2281 catcacgcac gtggaaggcc ccatcgatcc gatccgcgat gcggagatcg tcgaaaccga
     2341 gttgatgctg gccgacctgg aaagcctgga gaagcggctg aacaccgcgc agaagcgcgc
     2401 g
//
LOCUS       DASSZH010000350         3339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_230114, whole genome shotgun sequence.
ACCESSION   DASSZH010000350 DASSZH010000000
VERSION     DASSZH010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230114"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VFN42_07605"
     CDS             <1..534
                     /locus_tag="VFN42_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HET8996516.1"
                     /translation="GHFGLALTRYAHFTSPIRRYADLLVHRALIEALKLGPGGLDKEE
                     AAHFPDAAEHITATERRAALAERDAVDRYLAAYMADKVGARFAARISGVTRFGVFVTV
                     AESGASGLVPVSSLPDDFWQHDERTQSLTGRRTRLVFRLAQDVEVRLAEASPVTGGLV
                     FHILQGTPQGERRSRRR"
     gene            549..2072
                     /locus_tag="VFN42_07610"
     CDS             549..2072
                     /locus_tag="VFN42_07610"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET8996517.1"
                     /translation="MLLLLLIAQAHADQVNKGAAARFDASTVTAVYAAALGFMEPRTL
                     EAVPVPQLTLWGLRGLTAIDPDLATDAYQGKLRLIEGGQLLSTETPPAQPTPLLWAAI
                     AARLSSTAFAASSAIRQAGTAGVIQSFFDELFNHLDPYSRYVGPQEAALDRANRNGSA
                     GLGLTLERRNNAVRVAAAVEDSPAATAGVQPGDIVLAVDGERVHGDAVRAERLIAGPN
                     ATAVRLSWRGKGGHMHVTELIRTMVPPETVFADRQKNILMIRVTGFDRSTAAHLGYAL
                     KRGMAADPPPDGIVLDLRDNRGGLLHEAVEAANELLPTGVVAYTIGRDPDASRIWLSG
                     PGQLAQDVPVVVLVDGRTASAAEVLTAALTDRGRAVAVGSATLGKGLVQTITPLPNGG
                     ELFVTWSRILAPRGWPLQGLGVLPQVCTSLGGAALHKQLAALADGTQPMTAAIEAQRA
                     ARAPLPAATMVAIRRNCPAAGGNEDDMAAAKLLIENPAAYAAALLPPLQAPAAQAVR"
     gene            2145..2687
                     /gene="msrA"
                     /locus_tag="VFN42_07615"
     CDS             2145..2687
                     /gene="msrA"
                     /locus_tag="VFN42_07615"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET8996518.1"
                     /translation="MSEEIATLGGGCFWCLEAVFKDLRGVTSVMSGYAGGKVDRPSYK
                     QVCSGTTGHAEVVQVKFDPDQLAYADLLRVFFTIHDPTTRDRQGNDIGPQYRSIILAH
                     SDAQRHTAEQVMQEITAAKLWSRPLVTEVVALTTFYEAEPEHHDYFARNPWSGYCQVV
                     IAPKVAKFRKQFLDRLKAAA"
     gene            2797..3321
                     /locus_tag="VFN42_07620"
     CDS             2797..3321
                     /locus_tag="VFN42_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996519.1"
                     /translation="MPAVWGFASGQICPPEAPLCSQGSWRVFEEASPWGSPVHKTAIL
                     LSVLVGIGCAGCTQNNTAPQTAAATTPMTTGPAVTTYDGVYSGDPTRDPAAPASCLPP
                     TYQTLTVRNGSATLAGDMRTGQVAADGKLAMVGMRNGGVARVDGAFRAGKFDGVSSYP
                     ASGCKYIWHLVKEH"
BASE COUNT          551 a         1110 c         1168 g          510 t
ORIGIN      
        1 ggccatttcg gcctggcgct gacgcgctac gcgcacttca ccagcccgat ccgccgctat
       61 gccgacctgc tggtgcaccg cgcgctgatc gaggccctga agcttggccc cggcgggctg
      121 gacaaggagg aagccgcgca cttcccggac gccgcggagc acatcaccgc caccgagcgc
      181 cgcgccgccc tggccgagcg ggacgcggtg gaccgttacc tggctgcata catggcggac
      241 aaagttggcg cgcgtttcgc tgcgcgaatt tccggggtga cgcgattcgg ggttttcgtc
      301 actgtcgcgg aaagtggcgc gagcggtctc gtgccggttt ccagcctacc ggatgacttt
      361 tggcagcacg acgaacggac gcagagcttg accggccgcc gcaccagact ggtgttccgg
      421 ctggcgcagg atgtcgaggt gcggctggcc gaggccagtc ccgtcaccgg cggcctggtg
      481 ttccacattt tgcaaggcac accccaggga gaaaggcggt cgcggcgaag gtgacccgga
      541 ttggcgcatt gctgctgctt ctgctgatcg cgcaggcgca tgccgatcag gtcaacaagg
      601 gcgccgccgc acgatttgac gcctcgaccg tcaccgcggt gtacgcggcg gcgcttggct
      661 tcatggaacc gcgcacgctg gaagcggtgc cggtgccgca attgacgctc tgggggctcc
      721 gcggactgac cgcgatcgat cccgacctgg cgacggacgc gtatcagggc aagttgcggc
      781 tgatcgaggg tggccagctt ctgtcgacgg aaacgccacc cgctcagccc acgccgctgc
      841 tgtgggccgc catcgccgcg cgcctgtcca gtaccgcctt cgcagcttcg tccgcgatac
      901 ggcaagccgg caccgcgggc gtgatccaga gctttttcga cgagctgttc aaccacctgg
      961 atccctattc ccgctacgtc ggtccgcagg aagccgcgct ggaccgggca aatcgcaacg
     1021 gcagcgcggg gcttggattg acgctggagc ggcgcaacaa tgcggtgcgc gttgccgccg
     1081 cggtggaaga cagtcccgcg gcaaccgcgg gcgtgcagcc cggggacatc gtgctggcgg
     1141 tggatggcga acgggtccac ggcgatgccg tccgcgccga gcggctgatc gccggcccga
     1201 acgccaccgc ggtgaggctg agctggcgcg gcaagggcgg ccatatgcat gtcaccgagc
     1261 tgatccgtac catggtgccg ccggagacgg tgttcgctga ccgccagaag aacatcctga
     1321 tgatccgggt gaccggcttc gatcgcagca ccgccgcgca tctgggttac gccttgaagc
     1381 gcggcatggc agccgacccg ccgccggacg gcattgtgct ggatctgcgc gacaaccgtg
     1441 gcggcctgct gcatgaagcg gtggaagcgg cgaatgaact gctgccgacc ggtgtggtcg
     1501 cctacacgat cggccgggat cccgatgcca gccgcatctg gctgtccggc ccgggccaac
     1561 tggcgcagga cgtgccggtg gtggtgctgg tggacgggcg caccgccagc gcggccgagg
     1621 tgctgaccgc cgcgctgacg gatcgcggcc gagccgtcgc ggtgggaagc gcgacactgg
     1681 gcaaggggct ggtgcagacc atcacgccgc tgcccaacgg cggcgagttg ttcgttacct
     1741 ggagccgcat cctggcgcca cgcggctggc cgctgcaggg gctgggcgtg ctgccgcagg
     1801 tctgcaccag cctgggcggc gcagcattgc acaagcagct tgccgcgctg gccgacggaa
     1861 cgcagccgat gacggcagca atcgaggcgc agcgcgcggc ccgggcgccg ctgccggccg
     1921 cgacgatggt ggcgatccgg cgcaactgtc ctgcggccgg cggcaatgag gacgacatgg
     1981 ccgccgcgaa gctgctgatc gagaaccccg cggcctatgc tgcggccctg ctgccccccc
     2041 tgcaggcccc ggccgcacag gccgtgcgat agcaccggcc ggctttggcg cgcggcggcg
     2101 gcccggctat gatgcgcgtc aaccgaagca agggagccaa ggatatgagc gaagagatcg
     2161 cgacgctggg cggcggctgc ttctggtgcc tggaagcggt gttcaaggat ctgcgcggcg
     2221 tcacctcggt gatgtccggc tatgccggcg gcaaggtgga ccggccaagc tacaagcagg
     2281 tatgcagcgg cacgaccggc catgccgaag tggtgcaggt gaaattcgat cccgaccagc
     2341 ttgcctatgc cgatctgctg cgggtgttct tcaccatcca cgaccccacc acgcgcgacc
     2401 ggcaaggcaa cgacatcggg ccgcaatatc gctccatcat cctggcgcat tctgacgcgc
     2461 agcggcacac cgcggaacag gtgatgcagg agatcaccgc ggcaaagctg tggtcgcggc
     2521 cgctggtgac cgaggtggtg gcgctcacca cgttctatga ggccgaaccc gagcaccacg
     2581 attatttcgc ccgcaatccg tggagcggct actgccaggt ggtgatcgcg ccgaaggtgg
     2641 cgaagttccg caagcagttc ctcgatcggc tgaaggcggc ggcttagcag cagccccgat
     2701 ccgggcgttg gcacgcgctg ccggtcaggc gcgcccacgc cgaaagtggt gcggagctta
     2761 gcaaatcagg tgacatcccc catttgggtg atgcgcatgc cggcggtatg gggatttgct
     2821 tccggtcaga tctgcccgcc ggaagctccg ctctgctcac aaggctcctg gcgggtgttt
     2881 gaagaagcat cgccatgggg aagtccagtg cataagaccg cgatcttgct ttcggttctg
     2941 gtgggtatcg gctgcgccgg ctgcacccag aacaacaccg caccgcagac cgccgccgca
     3001 accaccccga tgaccaccgg ccccgccgtt accacctacg atggcgtcta tagcggcgac
     3061 ccgacgcggg acccggcggc accggcgtcc tgcctgccgc ccacctacca gaccctcacg
     3121 gtcaggaacg gcagcgcgac gctggcaggc gacatgcgta ccggccaggt cgcggcagac
     3181 gggaagctgg ccatggtcgg catgcgcaac ggcggcgtgg cccgggtgga cggcgcgttc
     3241 agagccggaa aattcgacgg cgtgtcgtcg tatccggcct caggctgcaa atacatctgg
     3301 catctcgtca aagagcattg agcgaggcgc cgccccatg
//
LOCUS       DASSZH010000351         2943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_110664, whole genome shotgun sequence.
ACCESSION   DASSZH010000351 DASSZH010000000
VERSION     DASSZH010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110664"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..1184
                     /locus_tag="VFN42_07625"
     CDS             198..1184
                     /locus_tag="VFN42_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009870292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET8996520.1"
                     /translation="MLGGIARLASGIGDELRQRLPGQRKTQRDKLALLIATMLDVRSA
                     NLMALAAALPRSAERADMRYQWISRFIANPLVDCDAVMAPFAGEVLAQAAARGGVVLI
                     MDQTKVSDRHQVLMLSLRFGERALPLAWRVEATAGAIGFARQRDLLDAITAWLPPEAD
                     ICLMADRFYGTPDLIALTIAKGWRYRLRLKGNLKVTIGGHTSPLAQHASRRLPYLTGV
                     ALTHRRIVTNIGIINDPGHEEPWLIAMSDPPGYLTTLDYAARWGIEPMFSDFKSRGFG
                     LAQSQLRTPDRLARLLLVMSLALYFAVSTGLWDAAANASADEKKAAGVNQET"
     gene            complement(1350..2780)
                     /locus_tag="VFN42_07630"
     CDS             complement(1350..2780)
                     /locus_tag="VFN42_07630"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HET8996521.1"
                     /translation="MSTDLDLSKLTHAEKGALILTLLARLDEAHKLIAELRARIDDLT
                     RPGKTPDNSSVPPSKGQKPNRPDTARRDGPRKGSLGRTGGGRALTAEPDEVVVAKAMR
                     CRHCQAAFSDADQMLDARYDKIDLPKLRPVVTQVERYAGRCRCCGGTTLAALPEGLEP
                     GTPFSLNIVALAMYLRFVHAVSYRRLSRLLLDLFALRISEGALDAAFRRGKPRFDADV
                     AAILARLRRARVICSDETGVRIDGRGCWNWAEPAKVPPMRSIGGKQAEGFQNAEVVIH
                     VVRHSRGADVVAEVLAGHRPALWVSDLSGAQQGHAEHWQVCLAHQLRDCQYAIEAGDA
                     VFAPRMKALLLRAVVLARRHRDLAETTRRAYRRQLEHALDAVMALAPSQRDGRRLRKR
                     YAKVRDHLFTFLEHPEIAADNNSSERELRPTATYRKVTGGFRSDWGADLFAAVRSVIG
                     TAARRGIDAYQAIRETLRRQSALAPG"
BASE COUNT          457 a         1023 c          976 g          487 t
ORIGIN      
        1 ggtatgcgcg tcttgtggtc tcggccagat cgcggtgccg ccgtgccagc accacggcgc
       61 gcagcagcag cgccttcatg cgcggggcga acaccgcgtc gccggcctga ccacccatca
      121 gttcgtcctg acctgagttg acgccccgaa tctgtgtgtg attcaaggag tccgcccaaa
      181 ttggagcggg gtctggaatg ctcggcggga ttgcgcggct ggcgagcggg atcggggatg
      241 agttgcggca gcggttgccg ggtcagcgca agacgcagcg cgacaagctg gcgctgctga
      301 tcgccaccat gctggatgtg cgcagcgcca acctgatggc cctggcggcg gcgttgccgc
      361 gcagcgccga acgggcggac atgcgctacc agtggatttc acgcttcatc gccaatcccc
      421 tggtggactg cgatgcggtc atggcgccgt tcgcgggcga ggtgctggcg caggccgccg
      481 cgcgcggcgg cgtggtgctg atcatggatc agaccaaggt cagcgaccgc catcaggtgc
      541 tgatgctgtc gctgcgcttt ggcgaacgcg cgctgccgct ggcgtggcgg gtcgaggcca
      601 ccgccggcgc gatcggcttc gcccggcaaa gagatctgct cgatgccatc accgcgtggc
      661 tgccgccgga ggccgacatc tgcctgatgg ccgatcgctt ctacggcacg cccgacctga
      721 tcgcgctgac catcgccaag ggctggcgct accggctgcg cctgaagggc aacctgaagg
      781 tcaccatcgg cgggcacacg tcgccgctgg cacagcatgc gtcccgccgg ctgccatacc
      841 tgaccggcgt cgcactgacg caccggcgca tcgtcaccaa catcggcatc atcaacgatc
      901 ccggccacga ggagccgtgg ctgatcgcca tgtccgaccc tcccggctat ctcaccacgc
      961 tcgactatgc cgcgcgctgg ggcatcgagc cgatgttctc cgatttcaag tcgcgcggct
     1021 tcggcctcgc gcagagccag ttgcgcaccc ccgaccgcct cgcgcggctg ctgctggtga
     1081 tgagccttgc cctctacttc gcagtctcca ccggcctgtg ggacgccgcc gccaatgctt
     1141 cggcggacga aaaaaaagcc gcaggcgtca accaagaaac ctgatccggg gcaggctgtc
     1201 ctggttcacc cgaggcattc gtcacatcgc cagattgctc cagttctgcc agcccttgcc
     1261 gcccctgtgg cagtccggat aaaactgatg agtggttagg ggcggcgctg ccgccggctc
     1321 ttcggcgtca gatcatcgac ggtaactgct caacccggtg cgagtgcgga ttggcggcgt
     1381 agggtttcac ggattgcctg ataggcgtcg atgccgcggc gagcggcggt gccgatgacg
     1441 gagcggacag cggcgaacag gtcggcaccc cagtctgagc ggaagccgcc ggtgaccttg
     1501 cgataggtgg cggtcgggcg gagttcccgc tcgctgctgt tgttgtcggc ggcaatctcg
     1561 ggatgttcga gaaaagtgaa caggtggtcg cgcaccttgg cgtatcgctt gcgcaggcgc
     1621 cggccgtccc gctggctggg ggccagcgcc atgacggcat ccagcgcatg ctccagttgc
     1681 cttcggtatg cgcgtcttgt ggtctcggcc agatcgcggt gccgccgtgc cagcaccacg
     1741 gcgcgcagca gcagcgcctt catgcgcggg gcgaacaccg cgtcgccggc ctcgatggcg
     1801 tattggcagt cgcgcaactg gtgcgcgagg cagacctgcc agtgttcggc gtggccctgc
     1861 tgcgcaccgg agagatcgga gacccacagc gccggacgat gcccggccag cacctcggcg
     1921 accacgtcgg caccgcggct gtggcggacc acatggatca ccacctcggc gttctggaac
     1981 ccctccgcct gctttccacc gatgctgcgc atcggcggaa cctttgcagg ctccgcccag
     2041 ttccagcagc cacgaccgtc gatgcgcacg ccggtttcgt ccgaacagat cacccgcgcg
     2101 cggcgcaggc gggcgagaat ggcggcaaca tcggcatcga accgcggctt gccgcggcgg
     2161 aacgcggcat ccagcgcgcc ctcactgatc cgcagggcga acaggtcgag cagcaggcgg
     2221 ctcagccgcc ggtagctgac cgcgtggaca aagcgcagat acatcgcgag cgcgacgatg
     2281 ttgaggctga acggcgtgcc cggctccaat ccctccggca gcgccgccag cgtggtcccg
     2341 ccgcagcagc ggcagcgtcc cgcataccgc tccacctgcg tcaccaccgg gcgcagcttc
     2401 ggcaggtcga tcttgtcgta gcgggcgtcg agcatctggt cggcatcgga gaatgccgct
     2461 tggcagtgcc ggcagcgcat cgccttggcc accaccacct catccggctc cgcggtcagc
     2521 gcccgcccgc cgcccgtgcg gccaagactg cccttgcgcg gaccgtcacg cctggccgtg
     2581 tccggccggt tcggcttctg ccccttcgac ggcggcacgc tggaattgtc cggcgtcttg
     2641 cccggccggg tgaggtcgtc gatccgcgct cgcaactcgg cgatcagctt gtgcgcttcg
     2701 tccagccgcg ccaacagggt taggatcagc gcgcccttct ccgcgtgcgt cagcttgctc
     2761 agatcaaggt cggtgctcac accaaccttg aatcgagtcg cccgttaccg cgtcaacccc
     2821 cttgcctccg tcacatcacc cacccccacc acacccgaca gaaaaccggg ttgagcagtt
     2881 accttggcgg caaattgcgg actgagggac gggcgctagg agagcgtgtt gaccgcctcc
     2941 ttg
//
LOCUS       DASSZH010000352         7054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_622454, whole genome shotgun sequence.
ACCESSION   DASSZH010000352 DASSZH010000000
VERSION     DASSZH010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7054
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622454"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..1457
                     /locus_tag="VFN42_07635"
     CDS             84..1457
                     /locus_tag="VFN42_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8996522.1"
                     /translation="MRTLSLAAGLAATPIVALAIMAALGWAAVWPALFAMGGSLLAAS
                     AIAALWRNDQALLGDALRRVAADAPPDARSPMPTLPSMQALQAEIERAARRVATRTAM
                     MEQLRRADTAILERLPDPLLVLGDDRNVRRFNSAARAAFGADMAAVLRHPGLRAAIDR
                     AFTLGTQQTAELSLPVPVPRDVHATVVPMDPPLADGGRAVLVLSDRTRERLVERMRAD
                     FVANASHELRTPLASLIGFVETLRGPAADDPPAQQRFLAIMAEQGARMNRLIDDLLSL
                     SRIELMEHQPPADRLDLAELLPSFLAGFEPRLRQASVRLETQIAEALPYVVGDADQLA
                     QVLQNLLDNAVKYGGEHHTVRLAVQPSAPDGRWPARPGVVIAVSDQGPGIPREHLPRL
                     TERFYRVDKGRSRAAGGTGLGLAIVKHIVNRHRGQLLIESEEGAGTTVSVWLPQAAED
                     QPRPGPA"
     gene            1516..1857
                     /locus_tag="VFN42_07640"
     CDS             1516..1857
                     /locus_tag="VFN42_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996523.1"
                     /translation="MNAVPFDTLKMAQRLQVAGFSAPQASGAAEALAEALTGANLATK
                     GDVAALGNKIEADNAVARSETKADNARLRTELMAAVELLRRDMTIKLGGIVIVATGVL
                     LAAMRYMTLHP"
     gene            complement(1963..2484)
                     /gene="infC"
                     /locus_tag="VFN42_07645"
     CDS             complement(1963..2484)
                     /gene="infC"
                     /locus_tag="VFN42_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014105241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET8996524.1"
                     /translation="MPAAPTRDGPRVNEEIRSPQVRLIDQDGEMQGVLSAREAQNRAF
                     QVGLDLVEISPNADPPVVKILDYGKFKYEQQKKKNEAKKKQKVIEIKEIKVRPNIDEN
                     DYQVKLRAMKMFIDEGDKVKVTLRFRGREMAHQDIGVKVLERIRNEMEPTSKVEQMPR
                     LENRQMVMVLSPR"
     gene            complement(2581..4530)
                     /gene="thrS"
                     /locus_tag="VFN42_07650"
     CDS             complement(2581..4530)
                     /gene="thrS"
                     /locus_tag="VFN42_07650"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET8996525.1"
                     /translation="MPAITLPDGSVRRFDAPVTGTTVAEAIGPGLARAALAMKLDGKL
                     VDLATQIDRDASVVFVTRKDADALELIRHDAAHVLAEAVQELYPGTQVTIGPSIENGF
                     YYDFARNEPFTPEDFAPIEAKMREIIARGEKFVRQVIDREDAIRFFRDKGEKYKAEII
                     QDLPPTETITLYRQGDWVDLCRGPHMRSTADVGGAFKLMKVAGAYWRGDHRNAMLSRI
                     YGTAWRDQKELDAYLHMLEEAERRDHRRIAKEMDLFHLQEEAAGSIFWHPKGWRLYLA
                     AEAYMRRRLDAAGYQEVKTPQLVDRSLWQASGHWDTFRQHMFIATVEEEDKILAVKPM
                     NCPCHIQIFRQGIRSYRELPLRLAEFGSCHRYEPSGALHGIMRVRAFTQDDAHIFCTE
                     DQIAPETVRFVELLASIYRDFGFPEFRVKFADRPDKRVGSDAVWDHAEAALKEACTTA
                     GVEYELNPGEGAFYGPKLEFVLRDAIGRDWQCGTLQVDFNMPQRLDAEYVAEDGARRR
                     PVMLHRAILGSFERFLGILIEQYAGRFPLWLAPVQAVVATIVSDADEYALEVAAALRR
                     AGLQVETDLTNQKINAKIREHSVAHVPVLVVVGRKEAEARTVALRRLGGAAQEIVSLD
                     AAIARLAAEATPPDLKQAETRLVAA"
     gene            complement(4690..5934)
                     /locus_tag="VFN42_07655"
     CDS             complement(4690..5934)
                     /locus_tag="VFN42_07655"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET8996526.1"
                     /translation="MAPLRLLFPLLLLVSLGRRVQAEALPVFAPGGPDAAAYGAELGY
                     PTAPPSARLPQRFMVGDYSAFDRLLPSHRVARPPAPSPLHRAAQELALTYRFRGNTRT
                     LRDYLDHNPATGLLIARGDTILFEHYQYGRTDRDRLLSQSMAKTVTAMLVGIALSEGA
                     IRSIDQPAADYVPRLAGTEFGRTTIRDLLHMASGVAFQELYNGEDDSAALNRALFSGI
                     DPADAVAQFNTRVVPAGTRFHYAGIQTEILGLVLAHATHGSLAAYLQSRIWQPMGAEA
                     DASWITDRTGHELAFCCISATLRDWARFALLLAHDGAWNGRQIIPRDWLLASTTVAAP
                     FLAPGRVARLYGYGNQVWLLPGPRREFALLGIHGQTIFVDPAAQLVLVQTAVRVKAAN
                     DPGAAELRAVWHALVRQVGETP"
     gene            6159..6743
                     /locus_tag="VFN42_07660"
     CDS             6159..6743
                     /locus_tag="VFN42_07660"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HET8996527.1"
                     /translation="MEETLVASEGTTIASGGGLADRYAAALYSHAEDVHALDEVVAQI
                     EGLGRLIDASADFRRLLESPLIDVNQATKAAQAVLQQEGFGKPVRDFVGVIAANRRLR
                     NLREIVAAFARLVAERRGIITAHVASAHPLTDVQRQQLRARLIEAGYGNVNISESVEP
                     DLLGGLVVRVGARLYDTSLKSRLQRLQYAMKGAA"
     gene            6743..>7054
                     /locus_tag="VFN42_07665"
     CDS             6743..>7054
                     /locus_tag="VFN42_07665"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553707.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET8996528.1"
                     /translation="MEIRPAEISEILKKQIAAFDTEANVAETGQVLSVGDGIARVFGL
                     QNVMAGEMVELPGAGLRGMALNLETDNVGVVIFGDDRQIREGDTVSRTGSIVDVPVGD
                     AL"
BASE COUNT         1059 a         2393 c         2436 g         1166 t
ORIGIN      
        1 aacgacccct ccccccaacc ccctcccaca aggggagggg gagtgttgtg ttcttccccc
       61 gcgctggcgg ctgacccgcc gccatgcgca ccctgtcgct cgctgccggt cttgccgcga
      121 ccccgatcgt ggcgctcgcg atcatggcgg cgcttggctg ggccgccgtc tggcccgctt
      181 tgttcgccat gggtggcagc ctgctcgcgg cgagtgccat cgcggcgctg tggcgcaacg
      241 accaggcgct gctgggtgac gccttgcgcc gggtcgcggc cgatgccccg cccgacgcac
      301 gctcgccgat gccgacgctg ccctccatgc aagcgctgca ggccgagatc gagcgcgccg
      361 cccggcgcgt cgccacccgc acggcgatga tggagcagct tcgccgcgcc gacaccgcga
      421 ttctggagcg cctgcccgat ccactgctgg tgctcggcga cgatcgcaat gtccgccggt
      481 tcaattcggc ggcgcgggcg gcgttcggcg cggacatggc ggcggtgctg cggcatcccg
      541 gcttgcgcgc cgcgatcgac cgggccttca ccctgggcac ccagcagacc gcggaactca
      601 gcctgccggt gccggtgccg cgcgacgtgc atgcgacggt ggtgccgatg gacccgccgc
      661 tggcggatgg cggccgcgcg gtgctggtgc tgtccgaccg cacgcgggag cggctggtgg
      721 agcggatgcg ggccgatttc gttgccaatg ccagccacga actgcgcacg ccgctcgcct
      781 cgctgatcgg cttcgtggag acgctgcgcg gtccggcagc ggacgatccg ccggcgcagc
      841 agcgcttcct ggcgatcatg gccgagcagg gtgcgcggat gaaccggctg atcgacgacc
      901 tgctgagcct gtcgcgcatc gagctgatgg aacaccagcc accggccgac cggctggatc
      961 tggcagagct gctgccgtcc ttcctggcag ggttcgagcc gcggctgcgg caggcatcgg
     1021 ttcggctgga gacgcagatc gcggaggcgc tgccctatgt ggtgggtgat gccgaccagc
     1081 tcgcgcaggt gctgcagaac ctgctggaca atgccgtgaa atatggcggc gagcaccaca
     1141 cggtgcggct ggcagtgcag ccttccgccc ccgacggacg ctggccggcg cggcccggcg
     1201 tggtgatcgc ggtatccgac cagggaccgg gcattccgcg ggaacatctg ccgcggctga
     1261 ccgagcgctt ctaccgggtg gacaaggggc ggtcacgtgc cgcgggcggc accgggctgg
     1321 ggctggcgat cgtcaagcac atcgtcaacc ggcaccgcgg ccagttgctg atcgaaagcg
     1381 aggaaggcgc tggcaccacc gtctcggtct ggctgccgca ggcggccgag gatcagccgc
     1441 gtcccgggcc ggcttaaccg ggcgtcaagc cttgtatgct ggactggcgg tctgtcctgg
     1501 cattaggatt ccaacatgaa cgcggtcccg ttcgacacgc tcaagatggc acagcggctg
     1561 caggtcgcgg ggttctccgc gccgcaggcg agcggggcgg cggaagccct ggccgaggcg
     1621 ctcaccggcg cgaatctcgc caccaagggc gatgtcgccg ccctgggcaa caagatcgag
     1681 gcagacaacg ccgtggcccg gtctgaaacg aaggccgaca atgccaggct gcgcactgag
     1741 ctgatggcgg cggtcgaact gctgcggcgg gacatgacga tcaagctggg cggcatcgtc
     1801 atcgttgcca ccggcgtgct gctggccgcc atgcgctaca tgacgctgca cccttgaccg
     1861 gggcatgttt gacctgacgg ggcgttcctg agagggcctg acgcgcagca caaccggcag
     1921 cccgctgtgg cggctgccgg ttgatcaggc ggtcgtgggc gctcagcgcg ggctgagcac
     1981 catcaccatc tgccggtttt ccagccgggg catctgctcc accttgctgg tcggctccat
     2041 ctcatttcgg atccgctcca gcaccttcac gccgatatcc tggtgggcca tttcacggcc
     2101 gcggaagcgc agcgtcacct tgaccttgtc cccctcgtcg atgaacatct tcatggcgcg
     2161 tagcttcacc tgatagtcgt tctcatcgat gttcggtcgg accttgatct ccttgatctc
     2221 gatgaccttc tgcttcttct tggcttcgtt ctttttcttc tgctgttcgt atttgaactt
     2281 gccatagtcc aggatcttga ccacgggtgg atcggcgttc gggctgattt ccaccagatc
     2341 cagtccgacc tggaacgcgc ggttctgcgc ctcgcgggcg ctcagcacgc cctgcatctc
     2401 gccgtcctgg tcgatcagtc gcacctgcgg ggatcggatc tcctcgttca cgcgtggccc
     2461 gtcgcgggtc ggcgctgcgg gcatgggtcc tcttgctatg ggtctctcct gtggcagtta
     2521 cgggtatgga accggtcgcg gaccggccgt ggctagcatg tccgattatt catgcgaatt
     2581 tcaagctgcc acgagtcggg tttccgcttg cttaagatcg ggcggcgtgg cttccgctgc
     2641 aagccgggca atcgccgcat ccagcgagac gatctcctgc gcggcgccgc cgagccggcg
     2701 gagcgccacc gtgcgagcct ccgcctcctt gcggccgacc acaaccagca ccgggacatg
     2761 cgccacgctg tgctcgcgga tcttggcgtt gatcttctgg ttggtcaggt cggtctcgac
     2821 ctgaagcccg gcgcggcgca acgcagccgc cacttccagc gcgtattcat ccgcatccga
     2881 cacgatagtg gcgaccaccg cctgcaccgg cgccagccag agcgggaaac gcccggcata
     2941 ttgctcgatc aggatgccga ggaagcgctc gaagctgccc aggatggcgc ggtgcagcat
     3001 caccgggcgg cggcgggcgc cgtcctcggc cacgtattcc gcgtccagcc gctgcggcat
     3061 gttgaaatcc acctgcagcg tgccgcattg ccagtcgcgg ccgatcgcgt cgcgcagcac
     3121 gaattccagc ttggggccgt agaacgcgcc ttcgcccgga ttcagctcgt attccacgcc
     3181 ggcggtggtg caggcttcct tcagcgccgc ttcggcatgg tcccacaccg cgtcgctgcc
     3241 gacccgcttg tccggccggt cggcgaactt cacccggaac tccggaaagc cgaaatcgcg
     3301 gtagatcgac gccagcagct cgacgaagcg cacggtctcc ggggcgatct ggtcctcggt
     3361 gcagaagatg tgcgcgtcgt cctgggtgaa ggcgcgcacc cgcatgatgc cgtgcagcgc
     3421 accggacggc tcgtagcggt ggcaggaacc gaactccgcc aggcgcagcg gcaactcgcg
     3481 gtagctgcgg atgccctggc ggaagatctg gatgtggcag gggcagttca tcggcttcac
     3541 cgcgaggatc ttgtcctcct cctcgacggt ggcgatgaac atgtgctggc ggaaggtgtc
     3601 ccaatggccg gaggcctgcc acagcgagcg gtccaccagt tgcggggtct tcacctcctg
     3661 gtagccggcg gcgtccagcc ggcggcgcat ataggcttcc gccgccagat acagccgcca
     3721 gcccttcggg tgccagaaga tcgagccggc ggcttcctcc tggagatgga acaggtccat
     3781 ctccttggcg atgcggcggt ggtcgcggcg ctccgcttcc tccagcatgt gcaggtaggc
     3841 gtccagctcc ttctggtcgc gccacgccgt gccgtagatg cgcgacagca tggcgttgcg
     3901 gtggtcgccg cgccaatacg cgccggccac cttcatcagc ttgaacgcgc cgccgacatc
     3961 ggcggtggag cgcatgtgcg gaccgcggca cagatcgacc cagtcgccct ggcggtacag
     4021 ggtgatggtc tcggtcggcg gcaggtcctg gatgatctcc gctttgtact tctctccctt
     4081 gtcgcggaag aagcggatgg cgtcctcgcg gtcgatcacc tggcggacga atttctcgcc
     4141 gcgcgcgatg atctcccgca tcttcgcctc gatcggggcg aagtcttccg gggtgaaggg
     4201 ttcgttgcgg gcgaagtcgt aatagaagcc gttctcgatc gacgggccga tggtgacctg
     4261 ggtgccgggg tagagttcct gcaccgcctc ggccagcacg tgcgccgcat cgtggcggat
     4321 cagctccagc gcgtcggcgt ccttgcgggt gacgaacacc acgctggcgt cgcggtcgat
     4381 ctgcgtcgcg aggtccacca gcttgccgtc cagcttcatc gccagggcgg cgcgggcgag
     4441 gccgggcccg atcgcctcgg ccaccgtcgt gcccgtcacc ggcgcatcga agcggcgcac
     4501 ggaaccgtcg ggcagggtga tggcgggcat ggggcagggg cctctctggt ggtggatgat
     4561 cgctctctat ggcgtcgctg ccggggttgg gcaagcgcgt acccgtcagg gcaggcacac
     4621 cggccgggct gatgaccagc cgctcggcag cgaggcgagg gcacgcgggc cgaccgcccg
     4681 ctcgcaggtt cagggcgtct cgcccacctg ccgcaccagg gcgtgccaca cggcgcggag
     4741 ctcggcagct ccagggtcgt tggctgcctt cacccggacg gcggtctgca ccagcaccag
     4801 ctgcgctgcc gggtcaacaa agatcgtctg tccgtggatg cccagcaggg cgaattcgcg
     4861 ccgcggtccc ggcagcagcc acacttggtt gccgtagccg tagaggcggg caacccggcc
     4921 gggcgccagg aagggtgcgg cgaccgtggt ggacgccagc agccagtcgc gcggaatgat
     4981 ctgccgcccg ttccaggcgc cgtcatgcgc gagcagcagg gcaaaccgtg cccagtcgcg
     5041 cagggtcgcg ctgatacagc agaacgccag ctcgtggccg gtccggtcgg tgatccagct
     5101 cgcgtccgct tccgcgccca tcggttgcca gatgcggctt tgcaggtatg ccgctaggct
     5161 gccgtgcgtc gcatgggcga ggaccagccc aaggatttcg gtctgaatgc cggcatagtg
     5221 gaagcgcgtg cctgccggca ccacccgtgt gttgaactgc gcgaccgcgt ccgccgggtc
     5281 gatgccggag aacagcgccc ggttcagcgc ggcgctgtca tcctccccat tgtacaactc
     5341 ctggaacgca acgccggacg ccatgtgcag caggtcgcgg atcgtcgtgc ggccgaattc
     5401 ggttccggcc aatcgcggta cgtaatcggc cgcgggctgg tcgatggagc ggatcgcccc
     5461 ctcagacagc gcaataccaa ccagcatggc cgtcaccgtc ttggccatgg attgcgacag
     5521 cagccgatcc cggtcggttc gcccgtactg gtaatgctcg aacaggatcg tgtcgccgcg
     5581 ggcgatcagc aggccggtgg ccggattatg atcgaggtag tcgcgcagcg tgcgcgtatt
     5641 gccgcggaat cgataggtca gtgccagctc ctgcgccgcg cggtgcagcg gcgacggggc
     5701 cggcggccgt gcgacgcggt gcgagggcag caggcgatcg aaggcgctgt aatcgcccac
     5761 catgaagcgc tgcggcaggc gggccgaggg cggcgcggtc ggatacccaa gctccgcacc
     5821 gtaagcggcg gcatcgggcc cgccgggcgc gaagaccggc agggcttcgg cctgaactcg
     5881 gcggcccagc gacaccagca acagcagagg aaacagcagc cggagcggcg ccatgacggt
     5941 ctccctggcg ccgaggattg acgttttgta gcagccccgc aacagcctgg ttgatccctg
     6001 cccggcgggc ggagcggggg ctctggggca ggcgttaccg catgtccatg tgttgccagc
     6061 cccccgaccc catgctagac gcccgccgcc ggactggcct gccttgcgca ggcagggggc
     6121 gcctggacca cgcccgcgcc cgtgcgcggc cccaacatat ggaagaaacg ctcgtggcct
     6181 cagaaggcac gacaatcgca tcaggcggcg gcctggcaga ccgttacgcc gccgcgctct
     6241 actcgcacgc cgaagacgtc cacgcgctgg acgaggtcgt ggcgcagatc gagggcctcg
     6301 gccggctgat cgatgccagc gcggatttcc gccgcctgct ggagtcgccg ctgatcgacg
     6361 tcaaccaggc caccaaggcc gcgcaggccg tgctgcagca ggaaggcttc ggcaagccgg
     6421 tgcgcgactt cgtcggcgtc atcgcggcca accgccggct gcgcaacctg cgcgagatcg
     6481 tcgccgcctt cgcccggctg gtggccgagc gccgcggcat catcaccgca cacgtcgcct
     6541 cggcgcatcc gctgaccgac gtgcagcgcc agcagttgcg cgcccggctg atcgaggccg
     6601 gctacggcaa cgtcaacatc agtgaatcgg tcgagcctga cctgctcggc ggcctggtcg
     6661 tccgcgtggg cgcccgcctt tacgacacat ccttgaaatc ccgcctgcag cgcctgcagt
     6721 acgccatgaa gggagccgcc tgatggagat ccgcccagcc gagatctcgg agatcttgaa
     6781 gaagcagatc gccgcgttcg acaccgaagc gaatgtcgcc gagaccggcc aggttctgtc
     6841 ggtcggtgac ggcatcgccc gcgtgttcgg gctgcagaac gtcatggccg gcgagatggt
     6901 cgagttaccc ggtgccggcc tgcgcggcat ggcgctgaac ctggaaaccg ataacgtcgg
     6961 cgtggtgatc ttcggagacg acaggcagat ccgcgaaggc gacaccgtca gccgcaccgg
     7021 ctccatcgtg gacgtgccgg tgggcgatgc cttg
//
LOCUS       DASSZH010000353         2122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1661910, whole genome shotgun sequence.
ACCESSION   DASSZH010000353 DASSZH010000000
VERSION     DASSZH010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1661910"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VFN42_07670"
     CDS             <1..348
                     /locus_tag="VFN42_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HET8996529.1"
                     /translation="LQRERMVCTLVLAKGRFPGMPNSLDALCRRFNIDLSARTTHNAL
                     LDCKLLSEVYVELTGGRQRGLSLAAETAALPMVAYSHAGPRAPRPIVPKEAELAAHAA
                     FLERLKEPIWNAG"
     gene            complement(369..1277)
                     /locus_tag="VFN42_07675"
     CDS             complement(369..1277)
                     /locus_tag="VFN42_07675"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET8996530.1"
                     /translation="MSTSADSAAPRRLFGTRAEGLALLLFTACSWGATWPQAKFLLSE
                     IPPFTARAIPNTAGVLFAFAVAVALRENLRPPPGEWRWLMIYGVLNYGAFTVFSTLSL
                     VWLKASEAVIIVYTLPIWAGMLAWPMLGERPTPRRIGAMLLALGGVALLVGVGPDAAE
                     WHKLPGIACGFLAAWIFALATVVAKRHPLHMPPVAGVAWQAAFGALPVVVLAVLFEHP
                     HWQRLTPLGFAAFGYIATVPLTIAYLTWFRALRLVPASIAATTVLISPTVGVISSALL
                     LGDPLGPRQIVALGMTLTGVGLAARG"
     gene            complement(1261..1710)
                     /locus_tag="VFN42_07680"
     CDS             complement(1261..1710)
                     /locus_tag="VFN42_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996531.1"
                     /translation="MPGSGPRRIFVRASSFLALGLLAGCDLVGQNTFAPAPPPRPAPV
                     AAQAPASAPSFEQRRALVTIDEITPVPDYRPLLHYAVRAAEARDRGVQFDVVAVLPDL
                     SAAEAGQREASAVMRAIMADGVPPTRIHLGLHAEPGLAHRQVRVYVR"
     gene            1949..>2122
                     /locus_tag="VFN42_07685"
     CDS             1949..>2122
                     /locus_tag="VFN42_07685"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HET8996532.1"
                     /translation="MDGTPQIIPRLDSRGARQQTLKAAIGCVGTGLHSGRRINLTLHP
                     AEPGSGIVLRRSDL"
BASE COUNT          376 a          738 c          730 g          278 t
ORIGIN      
        1 ctgcagcgcg aacgcatggt ctgcacgctg gtgctcgcca agggccgctt ccccggcatg
       61 ccgaacagcc tggacgcact gtgccggcgc ttcaatatcg acctgtcggc acgcaccaca
      121 cacaacgcgc tgctggactg caagctgctg tccgaggtgt acgtggaact cacgggcggc
      181 cggcagcgcg gcctgtcgct ggcggcagag acagcggcgt tgccaatggt ggcctacagc
      241 cacgccggtc cgcgcgcgcc gcggcccatc gtgccgaagg aggcggaact ggcggcgcat
      301 gcggccttcc tggaacggct gaaggagccg atctggaacg cggggtgacc tcgcccgggc
      361 cccctcacct atcccctcgc ggccaatcct acccccgtca gcgtcatccc cagcgccacg
      421 atttgtcgtg gccccagcgg gtcgccgagc agcagcgcgg agctgatcac gccgacggtg
      481 ggtgagatca gcacggtggt ggcggcgatc gacgccggga ccagccgcag cgcgcgaaac
      541 caggtcaggt aggcgatggt cagcggcacc gtcgcgatat agccgaaggc ggcaaaaccc
      601 agcggtgtca gccgctgcca gtgcggatgc tcgaacagca ccgccagaac caccaccggc
      661 agtgcgccga acgctgcctg ccaggcgact ccagccaccg gcggcatgtg cagcgggtgg
      721 cgcttcgcca ccacggtcgc cagcgcgaat atccaggcgg cgagaaagcc gcaggcgatt
      781 ccgggcagct tgtgccactc ggccgcatcc ggcccgacgc cgaccagcaa ggcgacgcca
      841 cccagcgcca gcagcatcgc gccaatccgc cgcggcgtcg gccgctcgcc caacatcggc
      901 caggccagca tgccggccca gatcggcagg gtatagacga tgatcaccgc ttccgaggct
      961 ttcagccaca ccagtgaaag cgtgctgaac acggtgaagg caccgtagtt cagcacgcca
     1021 tagatcatca gccagcgcca ctcgcccggg ggcggacgca ggttctcccg caaggccacg
     1081 gcgaccgcga aggcgaacag cacgccggcg gtgttgggga tggcgcgggc ggtgaaaggg
     1141 ggaatctcgc tgagcaggaa cttcgcctgc ggccaggttg cgccccagct gcaggcggtg
     1201 aacagcagca gagccaggcc ttccgcccgg gtgccgaaca accgccgcgg cgccgcgctg
     1261 tcagcggacg tagacacgga cctgacgatg agcgagaccc ggttccgcat gcaggccgag
     1321 gtggatccgc gtcggcggta cgccgtcggc catgatcgcg cgcatcaccg cgcttgcctc
     1381 tcgctgccct gcttccgccg ccgacaggtc cggcagcacc gcgacgacgt cgaactgcac
     1441 gccgcggtcg cgtgcttcgg cagcgcggac cgcgtagtgc agcagcggcc ggtagtccgg
     1501 taccggggta atctcgtcga tggtcaccag tgcgcgccgc tgttcgaagg acggcgccga
     1561 tgcgggggcc tgggccgcga ccggagcagg cctgggcggg ggggccggcg cgaaggtgtt
     1621 ctggccgacg agatcacagc cggcaagcag gcccagggcc aggaagctac tcgcccgaac
     1681 gaagatccgg cgcggaccac tgcccggcat gcgaaagact ccactcattc cgcggcaagc
     1741 ttgcaggaac ctgcgccggc ggcaagccgg ccgccgggcg gttggcgcga cgcctgtaac
     1801 atggcgcctg tgcggcgcag ctaatccaag aaatacaatc acttgcgtgg taacattcag
     1861 caacaatcct tgactcgcga tcggcccggc ggatcgctag cgtgccattg acgcgcgact
     1921 ccgcgccgtt tgctgaaaga cgcctgtcat ggacggaacg ccgcagatca tacctcgcct
     1981 ggacagccgg ggcgcccggc agcagacgct gaaggcggcg atcggctgcg tgggcactgg
     2041 gctgcattcc gggcggcgga tcaatctgac gctgcatccg gcggaaccgg gtagcggcat
     2101 cgtgctgcgc cgctccgacc tt
//
LOCUS       DASSZH010000354         2019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_417249, whole genome shotgun sequence.
ACCESSION   DASSZH010000354 DASSZH010000000
VERSION     DASSZH010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417249"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1020)
                     /gene="hemB"
                     /locus_tag="VFN42_07690"
     CDS             complement(49..1020)
                     /gene="hemB"
                     /locus_tag="VFN42_07690"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011387902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HET8996533.1"
                     /translation="MPPQFPTSRLRRLRASETLRDLMQETTVSLNDLIYPIFVEEELD
                     DYAPVESMPGIYRIPERKLDSAMKEIARAGVRAVMTFGVIHHKDDVGSDTLHGNGLMA
                     RMLKRAKDATPEMLVISDTCFCEYTSHGHCGVIVDGHVHNDHTIENLGRQAVIAAQAG
                     ADMIAPSAMMDGQIAAIRSALDGSGFTNTPIMAYSSKFASAFYGPFRAAAGCELKGDR
                     KAYQMNPMNGREALRESIADEAEGADILMVKPALAYLDIISAVKQRSLLPVAAYQVSG
                     EYAMIKFAAQAGAIDEQRVVQETLGSIKRAGADLILTYFAMDVARQG"
     gene            1194..1877
                     /locus_tag="VFN42_07695"
     CDS             1194..1877
                     /locus_tag="VFN42_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HET8996534.1"
                     /translation="MINKIVQSMAEAMAGIKDGSTVLVGGFGAVGQPNALIDGLIEQG
                     AKDLTIACNNAGVGRVGLARLMDLGRVRKIICSFPRSSDPVVFETLYRAGKIELEIVP
                     QGTLAERMRAAGAGVPAFFTATGVGTKMAVGKEHREIDGRTYILEQALPGDVALVEAW
                     ESDRWGNLTFKESGRNFNPVMAMAGKLTIVQTQHLRELGEINPDHVVTPGIFVHRVLH
                     VPYGDPTGF"
     gene            1897..>2019
                     /locus_tag="VFN42_07700"
     CDS             1897..>2019
                     /locus_tag="VFN42_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate CoA-transferase"
                     /protein_id="HET8996535.1"
                     /translation="MPDTAVSDIKPLTRPQMAARVAQDIPEGWYVNLGIGIPTAV"
BASE COUNT          343 a          717 c          633 g          326 t
ORIGIN      
        1 gagcgcacca accagaccga tgcagcaggg atccccggaa cagggccttt atccctgccg
       61 cgcgacatcc atcgcgaaat aggtcaggat cagatcggcg cccgcccgct tgatggagcc
      121 aagcgtctcc tgcaccacgc gctgctcgtc gatcgcgccg gcctgcgcgg cgaacttgat
      181 catcgcgtat tcgccgctca cctggtacgc cgccaccggc agcaggctgc gctgcttcac
      241 cgcgctgatg atgtccagat acgccagcgc cggcttcacc atcaggatat cggcgccttc
      301 cgcctcatcg gcaatcgact cgcgcagcgc ctcgcgcccg ttcatcgggt tcatctggta
      361 cgccttgcgg tcgcccttca gctcgcaccc cgccgcggca cggaacggcc catagaaggc
      421 cgaggcgaat ttcgacgagt acgccatgat cggcgtgttg gtgaacccgc tgccgtccag
      481 cgcggaacgg atcgccgcaa tctgcccgtc catcatcgcg ctgggcgcaa tcatgtcggc
      541 gccggcctgc gccgcgatca ccgcctggcg gccgagattc tcgatggtgt ggtcgttgtg
      601 cacgtgccca tcgacgatca cgccgcaatg cccatgcgac gtgtattcgc agaagcaggt
      661 atcggagatc accagcatct ccggcgtggc atccttggcg cgcttcagca tgcgcgccat
      721 cagcccgttg ccgtgcaggg tgtcgctgcc gacatcgtcc ttgtggtgga tcacgccgaa
      781 ggtcatcacc gcgcggacgc cggcgcgggc gatctccttc atcgcggaat ccagcttgcg
      841 ctccgggatg cggtagatgc cgggcatgct ctcgaccggg gcgtagtcgt ccagctcctc
      901 ctcgacgaag atcggataga tcaggtcgtt gagcgagacg gtggtctcct gcatcagatc
      961 gcgcaacgtc tcgctggcac gcaggcggcg caggcggctg gtcgggaact gcggcggcat
     1021 gggcccctcc ttcagcaaac gcggtcgggc gcgctgcggc gtgccggcct tcacataagc
     1081 cacatgcggg gccgttgcca ctgacctatg tcagtacgag ggcgccggcc cggcttggcc
     1141 ccctgccccg cccgcgctaa accgacagca cggactccca agggaaaccg cgcatgatca
     1201 acaagatcgt ccagtccatg gccgaggcca tggcgggcat caaagacggc tccaccgtgc
     1261 tggtcggcgg tttcggcgcc gtcgggcaac ccaacgcgct gatcgacggg ctgatcgagc
     1321 agggcgccaa ggacctcacc atcgcctgca acaacgccgg agtcggccgc gtcgggctgg
     1381 cgcgcctgat ggacctcggc cgggtgcgca agatcatctg ctccttcccg cgcagctccg
     1441 acccggtggt gttcgaaact ttgtatcgcg ccggcaagat cgagctggag atcgtcccgc
     1501 aaggcaccct ggccgaacgg atgcgcgccg ccggcgccgg cgtgccggcc ttcttcaccg
     1561 ccaccggcgt tggcaccaag atggcggtcg gcaaggagca ccgggagatc gacggccgca
     1621 cctacatcct ggagcaggcg ctgcccggcg acgtggcgct ggtggaagcc tgggaatccg
     1681 accgctgggg caacctgacg ttcaaggaat ccggccgcaa cttcaacccg gtgatggcga
     1741 tggccggcaa gctgaccatc gtgcagaccc agcatttgcg cgaactgggc gagatcaacc
     1801 ccgaccacgt cgtcaccccc ggcatcttcg tgcaccgcgt gctgcacgtg ccgtacggcg
     1861 accccaccgg tttctgatcg cgacagagag gccaccatgc ccgataccgc cgtctccgac
     1921 atcaagccct taacccgccc gcaaatggcc gcgcgcgtcg cgcaggacat tcccgaaggc
     1981 tggtacgtca acctgggcat cggcatcccc accgccgtc
//
LOCUS       DASSZH010000355         3342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1423195, whole genome shotgun sequence.
ACCESSION   DASSZH010000355 DASSZH010000000
VERSION     DASSZH010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423195"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VFN42_07705"
     CDS             <1..477
                     /locus_tag="VFN42_07705"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8996536.1"
                     /translation="SFSFRLAARQPDYETANGSVRIADSHNFPASTTIAAALVEVQPG
                     GLRELHWHPNADEWQFWISGQARMTVFAANGRARTFDFHAGDVGVVPRAMGHYIENTG
                     RETVRYLELFRSGEYAEVPLTAWAKNTPHEFVAQHLNIDPALLKRLPEKKQPIVPA"
     gene            complement(497..1234)
                     /locus_tag="VFN42_07710"
     CDS             complement(497..1234)
                     /locus_tag="VFN42_07710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8996537.1"
                     /translation="MSERKRVLLLTGASRGIGHATVKTFEQAGWRVLTISRQAFDPRC
                     PWEGGRENHIQLDLADIGCVQAAMPRLRDFIGGGLDALINNAAISPKRPDGGKISALE
                     MGYADWLHIFNVNFFACMALVQGLRDPLEAAQGTVVNMTSIVGSRVHPFASAAYAASK
                     AALWALTRELAEEFGKSGVRVNAVSPGEIDTGILSPGTKAIVDRDIPLRRLGKPKEVA
                     DLLLFLCSPTAGYISGSEIHIDGGQRV"
     gene            1389..2348
                     /locus_tag="VFN42_07715"
     CDS             1389..2348
                     /locus_tag="VFN42_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET8996538.1"
                     /translation="MDRLLTSLRAAAEPTRLRLLALAARGAFCVTEFTEILGQSQPRL
                     SRHLKLLCESGLLERVREGANVWFTLPPNEEGSLARELTARLPPDDPILETDRRQAAR
                     VLAERARIASEKFRRQGADWDEMRALDLPAAEVEAALASLVPSTKPQRLLDIGTGTGR
                     VLELLAPAISQGIGVDASKAMLALARARLGRAGLSHCSVRLADMYRLPLPNTAFDTVV
                     LQMVLHHAEDPGGVLREATRVLRPGGRLIVIDLAAHARDDVAAKLAHRWPGFSDDAMH
                     ALLHAAGVAAGAALRVTGPLEVRLWPAMRPAAAELANTHELAV"
     gene            2361..>3342
                     /locus_tag="VFN42_07720"
     CDS             2361..>3342
                     /locus_tag="VFN42_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HET8996539.1"
                     /translation="MTRPHLLDALRDAVLLCDGGMGTRVQALTLDIERDFWGRENCTD
                     VLNLSRPDLVREIHRGYFEAGADMVETNTFGGSPVTLGEFDLADRAFELNRRGAELAR
                     EAAEQFSDDRHRYVIGSIGPGTKLPSLGNIAYDPLEQALTEQCRGLIAGGVDAILIET
                     CQDTLQIKAAVNGAKIARAAAGTDTPVFVQMTVETTGTLLVGPDVAAAATVVHALDVP
                     LIGLNCATGPQEMAEHVRWLAANWPGLLSVQPNAGLPELVDGQTHYPLGAAELASWLE
                     RFVAEDGVNLIGGCCGTSIPHITALDAMLRRRGGFRPAPVARKPVWIPSVA"
BASE COUNT          523 a         1179 c         1128 g          512 t
ORIGIN      
        1 tccttcagct tccgcctggc ggccaggcag cccgactatg aaaccgcgaa cggcagcgtc
       61 cgcatcgccg attcccacaa tttccccgct tccaccacga ttgccgcggc gctggtggag
      121 gtgcagcccg gcggcttgcg cgaactgcac tggcatccga acgccgatga gtggcagttc
      181 tggatcagcg gccaggcgcg catgacggtg ttcgcggcca acgggcgggc gcgcaccttc
      241 gatttccatg ccggcgatgt cggcgtggtg ccgcgggcga tgggacatta catcgagaat
      301 accgggcgcg agacggtccg ctatctggaa ctcttccgca gcggcgaata cgcggaagtg
      361 ccgctgaccg cctgggcgaa aaacacaccg cacgaattcg tggcgcagca cctgaacatc
      421 gacccggcgc tgctgaagcg tctgccggaa aagaagcagc cgatcgtacc cgcctgacgc
      481 cgcctgcgtc tggcggctat acccgctgcc cgccgtcgat atggatttcc gagcccgata
      541 tgtatcccgc ggtcggcgag cacaggaaca gcagcagatc cgccacctcc ttcggcttgc
      601 ccagccggcg cagcggaatg tcgcggtcca cgatcgcctt ggtgccgggc gacaggatcc
      661 cggtgtcgat ctcgcccggc gacaccgcgt tgacccgcac cccggacttg ccgaactcct
      721 ccgccagttc ccgcgtcagc gcccagagcg cggccttgga cgccgcgtag gcagcggaag
      781 cgaaggggtg cacccgcgac ccgacgatcg aggtcatgtt caccacggta ccctgcgccg
      841 cctccagcgg atcgcgcagc ccctgcacca gcgccatgca ggcgaagaag ttgacgttga
      901 agatgtgcag ccaatcggca tagcccatct ccagcgcgga gatcttgccg ccatccggcc
      961 gcttcgggga gattgcggcg ttgttgatca gcgcgtccag accgccgccg atgaaatcgc
     1021 gcagccgcgg catcgcagcc tgcacgcagc cgatatcggc caggtcgagc tggatgtggt
     1081 tttcccgtcc gccttcccag gggcaacgcg gatcgaacgc ctggcgggag atggtcagca
     1141 cgcgccagcc ggcctgctcg aaggtcttca ccgtggcgtg gccgatcccc cggctggcgc
     1201 cggtcagcag cagcacacgc ttgcgctcgg acatgacgct ggttctccgc gtttccgctt
     1261 ggccggagcg ccaccatagg cacccggatc gccgggtatt ctagcacgag gtggcgcccg
     1321 gcgaacggcc tcagcgcaca agccaccacc ccctggacat aagcatatct ttatgtcaga
     1381 gatgcgccat ggaccgcctg ctcaccagcc tgcgtgccgc cgccgaaccc acccgcctgc
     1441 gcctgctggc gctggcggcg cgcggtgcgt tctgcgtgac ggagttcacc gaaatcctcg
     1501 gccagtcgca gccccgcctg tcgcgccacc tgaagctgct gtgtgaatcc ggcctgctgg
     1561 agcgggtgcg tgagggcgcc aatgtctggt tcaccctgcc gccgaatgag gaaggcagcc
     1621 tggcgcgcga actgaccgcg cgcctgcccc ccgacgaccc gatcctggag accgatcgcc
     1681 gccaggcagc ccgcgtgctg gccgaacgcg cccgcatcgc ctcggagaaa ttccgccgcc
     1741 agggcgccga ttgggacgag atgcgtgcgc tggacctgcc ggccgccgag gtggaggcgg
     1801 cgcttgcctc ccttgtgccg agcaccaagc cgcagcggct gctggacatc ggcaccggca
     1861 ccggccgcgt gctggaactg ctggcgccgg caatctcgca gggcatcggc gtcgatgcct
     1921 ccaaggccat gctggccctg gcgcgcgccc gcctgggccg cgccgggctg tcgcactgct
     1981 cggtgcggct ggccgacatg taccggctgc cgctgcccaa caccgcattc gataccgtgg
     2041 tgctgcagat ggtgctgcat cacgcggagg accccggcgg cgtgctgcgt gaggctacgc
     2101 gcgtgctgcg cccgggcggc cgcctgatcg tcatcgacct tgccgcgcac gcgcgtgatg
     2161 atgtggcggc gaagctggcg catcgctggc cgggattttc cgacgacgcc atgcacgcgt
     2221 tgctgcacgc cgccggcgtg gctgccggcg ccgcgttgcg cgtgaccggt ccgctggagg
     2281 tgcggctgtg gccagccatg cgcccggccg cagccgaact ggccaatacc cacgaactcg
     2341 ccgtctgaaa gtccgaccga atgacccgtc cccatctgct ggatgcgttg cgtgatgccg
     2401 tattgctgtg cgacggaggc atgggcacgc gcgtccaggc gctgacgctg gatatcgagc
     2461 gcgacttctg ggggcgggag aactgcaccg acgtgctgaa cctgtcgcgc cccgacctgg
     2521 tacgcgagat ccatcgcggc tacttcgaag ccggcgccga catggtggaa accaacacgt
     2581 tcggcggctc gccggtgacg ctgggcgaat tcgacctcgc cgaccgcgcc ttcgaactga
     2641 accgccgcgg tgcggaactg gcgcgcgagg cggcggagca gttctccgac gaccggcatc
     2701 gctatgtcat cggcagcatt ggtccgggca ccaaactgcc aagcctgggc aacatcgcct
     2761 atgatccgct ggaacaggcg ctgaccgagc agtgccgcgg cctgatcgcc ggcggcgtcg
     2821 atgccatcct gatcgagacc tgccaggaca cgctgcagat caaggccgcg gtgaacggcg
     2881 ccaagatcgc ccgggcggca gccggcacgg atacgccggt gtttgtacag atgacggtgg
     2941 aaaccaccgg cacgctgctg gtcggccccg acgtcgccgc tgccgccacg gtggtgcacg
     3001 cgctggacgt gccgttgatc ggcctgaact gcgccaccgg cccgcaggaa atggcggagc
     3061 atgtgcgctg gctcgccgcg aactggcccg gcctgctgtc ggtgcagccc aatgccggtc
     3121 tgccggagct ggtggacggc cagacgcact atccgctggg cgccgcggaa ctggcgagct
     3181 ggctggaacg cttcgtcgcg gaggacggcg tgaacctgat cggcggctgc tgcggcacgt
     3241 cgatcccgca catcacagcg ctggacgcga tgctgcggcg gcgcggcggc ttccgcccgg
     3301 cgccggtcgc gcgcaagccg gtctggatcc cctccgtcgc cg
//
LOCUS       DASSZH010000356         4201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_247526, whole genome shotgun sequence.
ACCESSION   DASSZH010000356 DASSZH010000000
VERSION     DASSZH010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247526"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="VFN42_07725"
     CDS             complement(<1..1197)
                     /locus_tag="VFN42_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HET8996540.1"
                     /translation="MESVLGVGRSLSGRRWVWRSGEDRVGLGIAQRHGVPEIVGRLLA
                     ARGIDIDSACHFLEPTLRVLLPDPSLLTDMDAAAERLAHAVQRGETVAVFGDYDVDGA
                     CSSALMAVLLRALGCVVHTHVPDRLADGYGPNPTALRMLAERGSSLIVCVDCGTAAGE
                     ALGAVAGVADVIVLDHHKAEGPPPAVLATVNPNRLDDRSGLNFLCAAAVAFMTAVATV
                     RTLRRMGFFAQRAEPDLMGLLDLVALATVCDVMPLTGVNRALVCQGLKVMARRARPGI
                     AALLDVAQTRDKPSAFTCGFGLGPRINAAGRISEADLGLRLLLAQDPVEAAALANRLD
                     DVNRQRQEVEAGILDAALAATLDAVNRQRQEVEASMLEAAMTAAEAQAMKGYAALMIS
                     GPAWHPG"
     gene            complement(1208..2200)
                     /gene="glpX"
                     /locus_tag="VFN42_07730"
     CDS             complement(1208..2200)
                     /gene="glpX"
                     /locus_tag="VFN42_07730"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HET8996541.1"
                     /translation="MDAVTPKPGDVTDRNLALDLVRVTEAAALSASRWIGLGKKNDAD
                     GAAVEAMRRAFDVVPISGTVVIGEGEMDEAPMLYIGETVGAGGPAMDIAVDPLEGTTL
                     TAKGGPAALAVVALAEKGNFLHAPDIYMDKIAVGGGLPDDLVDLDAPVRENLRNLAKA
                     KRCEVSDLVACILDRDRHKELIAKTREAGARIMLISDGDVTGVVATATPDSEIDIYLG
                     WGGAPEGVLAAAALRCIGGQMQGRLIYENDEQIARTREMGHADPHKKFTVTELAKGDV
                     MFAATGVTTGPMLRGVKRFGHGALTHSMVMRSKSGTVRFVEARHNFTTKTWTEH"
     gene            complement(2352..3200)
                     /locus_tag="VFN42_07735"
     CDS             complement(2352..3200)
                     /locus_tag="VFN42_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996542.1"
                     /translation="MTRRCQLSILTDMHHKDTTTAGLPPLPGPFLDRRGRPVPEGIAY
                     VLTILHFLLAYGRHLSATLEQRATQRGFPLIARYFGTARVQFILARLSRGILRAMALE
                     RVLLARAARGRDLRARPPGECRKPRAKPPDTQGTAEAEQQKKARPAGRPDPDAALDLA
                     SLPSIEQLEAEIRRHPIGRALADICCDLGISSTLCDSRFGTVIYNAISWYRGNFGKYY
                     QEIRKREAAFLDELDQDRSFRFDWPEQHPEAAHRFLGFWIGEGAAALFPVLDPWPPQA
                     AADRPP"
     gene            complement(3228..3662)
                     /gene="msrB"
                     /locus_tag="VFN42_07740"
     CDS             complement(3228..3662)
                     /gene="msrB"
                     /locus_tag="VFN42_07740"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET8996543.1"
                     /translation="MKRLFGGSHDTADHDFPVQHSEDTWRQKLTPQQFSVLRKHGTER
                     PGTSKLNMEKRQGTFVCAGCGQKLFASETKFESGTGWPSFFAPLEGGVETSEDRTLWM
                     VRTEVHCSRCGGHLGHVFPDGPQPTGQRFCMNGVAMDFEPDA"
     gene            3766..>4201
                     /gene="tsaE"
                     /locus_tag="VFN42_07745"
     CDS             3766..>4201
                     /gene="tsaE"
                     /locus_tag="VFN42_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET8996544.1"
                     /translation="MTTRRFDLPDLAATGRLAAAVAALARAGDAILLDGPLGAGKTAF
                     ARAFLRAASGDPALEVPSPTFTLVQSYDTRLGPVHHFDLWRLAGPAALDELGWDEARD
                     DIVLVEWPDRLGGLRPPDALVLALQPGQAELRRAVVSGWPDRI"
BASE COUNT          594 a         1527 c         1405 g          675 t
ORIGIN      
        1 gcccggatgc cacgccggtc cgctgatcat cagcgccgca taacccttca tggcctgtgc
       61 ctcggcggcg gtcatcgccg cttccagcat cgaggcttcc acttcctggc gctgccggtt
      121 caccgcatcg agcgtcgcgg ccaatgcggc gtcgaggatg ccggcctcca cttcctggcg
      181 ctggcggttc acgtcgtcca gccggttggc cagcgccgcc gcttccaccg ggtcttgtgc
      241 cagcagcagg cgcaggccga ggtccgcctc actgatccgc cctgccgcgt tgatccgtgg
      301 ccccagtcca aacccgcagg tgaacgcgct cggcttgtcg cgtgtctgcg ccacgtccag
      361 cagcgcggcg atgcccgggc gcgcgcggcg cgccatcacc ttcagcccct ggcagaccag
      421 ggcgcggttc accccggtca gcggcatcac gtcgcagacc gtcgccagcg ccaccaggtc
      481 cagcagcccc atcagatcgg gttccgcccg ctgggcgaag aaccccatcc ggcgcagcgt
      541 ccgcacggtc gccacggcgg tcatgaacgc caccgccgcc gcgcacagga agttcagccc
      601 ggaccggtcg tccagccggt tcggattgac ggtggccagc acggcagggg gcgggccttc
      661 cgccttgtga tggtccagca cgatcacgtc ggcgacgccg gcgaccgcgc ccagcgcctc
      721 acccgcggcg gtgccgcaat cgacgcacac gatcaggctg ctgccgcgct cggccagcat
      781 gcgcagcgcg gtggggttcg gtccgtagcc atccgccagg cggtccggca catgcgtgtg
      841 cacaacgcag cccagtgccc gcagcagcac cgccatcagc gccgaactgc aggcgccgtc
      901 cacgtcgtag tcgccgaaca ccgcgaccgt ctcgccgcgt tgcaccgcgt gggccagccg
      961 ctccgccgcg gcgtccatgt cggtcagcag cgacggatcg ggcagcagca cgcgcagggt
     1021 cggttccagg aaatggcagg cgctgtcgat atcgatgccg cgcgccgcca gcagccggcc
     1081 gacgatctcc ggcacgccgt gacgctgcgc aatccccagt ccgacccgat cctcgccgct
     1141 gcgccacacc cagcgccggc cgctcaggct gcggccgacg ccaagcacgg attccactgc
     1201 cccgcgccta gtgttcggtc caggtcttgg tggtgaagtt gtgccgcgcc tcgacgaacc
     1261 gcactgtgcc ggacttgctg cgcatcacca tggaatgggt cagcgcgcca tggccgaacc
     1321 gcttcacccc gcgcagcatc ggcccggtgg tgacgccggt ggcggcgaac atcacatcgc
     1381 ccttcgccag ctccgtcacg gtgaacttct tgtgcggatc ggcatgcccc atctcgcggg
     1441 tccgcgcgat ctgctcgtca ttctcgtaga tcagccggcc ctgcatctgg ccgccgatgc
     1501 agcgcagcgc cgcggccgcc aacacaccct ccggcgcacc gccccagccc aggtagatgt
     1561 cgatctccga atccggagtc gccgtcgcca ccacgccggt cacgtcgccg tcgctgatga
     1621 gcatgatgcg cgcgccggcc tcgcgcgtct tggcgatcaa ctccttgtgg cggtcgcgat
     1681 ccaggataca ggcgaccagg tcggacacct cgcaacgctt ggctttcgcc aggttgcgca
     1741 ggttctccct caccggcgcg tccagatcga ccaggtcgtc cggcagcccg ccgccgaccg
     1801 cgatcttgtc catgtagata tccggtgcgt gcaggaagtt gcccttttcc gccagcgcca
     1861 ccaccgccag tgccgccggc ccgcccttgg cggtcagcgt ggtgccttcc agcggatcga
     1921 cggcaatgtc catcgccggg ccgccggcgc cgaccgtctc gccgatatac agcatcggcg
     1981 cttcgtccat ctcgccttcg ccgatcacca cggtgccgct gatcggcacc acgtcgaacg
     2041 cccggcgcat cgcttccacc gcggcgccgt cggcatcgtt ctttttgccc aggccgatcc
     2101 agcgcgacgc agacaaggca gccgcttctg tcacccgtac caggtcgagc gcgaggttgc
     2161 ggtcggtgac gtctcccggc ttcggcgtga ctgcgtccat gatacgttcc tccagcggtg
     2221 cgattccgcc ggaggatagc acgaaccggg cgcgcacggc aggcgggtca accgccgcca
     2281 ggaatcacca tggcggggct ggccgtcggc cggccccgtg catgacactg gcgatggatc
     2341 gtgcggcgcg gtcagggcgg ccggtcggcc gctgcttgcg gcggccaggg atccagcacc
     2401 gggaacagcg ccgccgcacc ctcgccgatc cagaatccca ggaagcggtg cgcggcctcg
     2461 ggatgctgct ccggccagtc gaaacggaag cttctgtcct ggtccagttc gtcgaggaac
     2521 gccgcctcgc gcttgcggat ctcctggtag tatttgccga aattgccgcg ataccaactg
     2581 atcgcgttgt agatcacggt gccgaaccgg ctgtcgcaca gcgtggacga gatgccgagg
     2641 tcgcagcaga tatcggccag tgcgcggccg atcgggtggc ggcggatttc agcttccagt
     2701 tgctcgatgc tcggcaggct ggccaggtcc agcgccgcgt ccgggtcggg gcggccggcc
     2761 ggacgggctt tcttctgctg ttccgcttcg gcggtgccct gcgtgtccgg cggcttcgcg
     2821 cgcggcttgc ggcattcgcc gggcgggcga gcgcggagat cgcggccgcg cgcggcgcgc
     2881 gccagcaaca cgcgttccag cgccatggcg cgcaggatgc cgcgcgacag gcgcgccagg
     2941 atgaactgga cccgcgccgt gccgaaatag cgggcgatca gcgggaagcc gcgctgcgtg
     3001 gcgcgctgtt ccagggtcgc gctcaggtgg cggccgtacg caagcaggaa gtggaggatg
     3061 gtgagaacat aggcgatccc ctccggcacg ggtctgccgc gccggtcgag aaagggaccg
     3121 gggaggggag gtaggccggc agtggtggtg tccttgtggt gcatgtcagt cagtatagat
     3181 aactgacagc gtcgtgtcaa ggactttttc ctaagccggc cggcttgcta cgcgtccggt
     3241 tcgaaatcca tggcgacgcc gttcatgcaa aagcgctggc cggtcggctg cggcccgtcc
     3301 gggaacacat gccccagatg cccgccgcat cggctgcaat gcacctcggt gcgcaccatc
     3361 caaagcgtgc ggtcctctga tgtctctacc ccgccctcca gcggggcgaa gaagctcggc
     3421 catcctgtgc cgctctcgaa tttcgtctcc gaggcgaaca acttctgccc gcagccggca
     3481 cagacgaagg tgccctgccg cttttccatg ttcagcttgc tggtgccggg ccgctcggtg
     3541 ccgtgcttgc gcaacacgga gaactgctgc ggcgtcagct tctgccgcca cgtgtcctcg
     3601 ctgtgctgca ccggaaaatc gtggtccgcc gtgtcgtggc tgccgccgaa caagcgcttc
     3661 atccttgcct cctcacgcaa gctttgtggt ccagcatgcg gcaagtcgcg gccggcgggc
     3721 aaaccccggt tgttcccgac tccggcgctg cggcagattg ccccgatgac cacccgccgc
     3781 ttcgaccttc cggatctcgc cgccaccggg cgcctcgctg ccgcggtggc ggcgctggcg
     3841 cgcgcgggcg atgcgatcct gttggatggg ccgctcggcg ccggcaagac cgccttcgcc
     3901 cgcgccttcc tgcgcgccgc cagcggcgac ccggcgctgg aggtgcccag cccgaccttc
     3961 accctggtgc aaagctacga cacgcggctg gggccggtgc atcatttcga cctctggcgg
     4021 ctggccgggc cggcggcgct ggacgaactc ggctgggacg aagcgcggga cgacatcgtg
     4081 ctggtggaat ggcccgaccg gctcggcggg ttgcgtccgc ccgatgcgct ggtgctggcg
     4141 ctgcagccgg ggcaggcgga actgcgccgc gccgtggtat cgggctggcc ggaccggatc
     4201 g
//
LOCUS       DASSZH010000357         2937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_758433, whole genome shotgun sequence.
ACCESSION   DASSZH010000357 DASSZH010000000
VERSION     DASSZH010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758433"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="VFN42_07750"
     CDS             <1..1186
                     /locus_tag="VFN42_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HET8996545.1"
                     /translation="PISLFTIDLSRRDYDEFYRGFANDTLWPVLHYQIGLHSFEWPQF
                     AAYRRVNALFAHSLAPLVQPDDVIWAHDYHLLCLADALRAAGLRNRIGLFLHTPFPAP
                     AVFMTIPAHAELIRAMCAYDLLGFQTEADRTAFTDYLRRHAGGEDRGGGTLRVFDRIL
                     RTGVYPIGVHIDEVRAQAEAPANRRTATRMRASVLEKLILSVDRLDYSKGLRQRFGAF
                     ERFLQDHPKWQGSVTLMQIAPPSRGDIQTYKEIRRELEAEAGRINGRFAEVDWVPLRY
                     LNRGFPRAVLMPLYAEAQVGLVTPLRDGMNLVAKEYVAAQDPDDPGVLVLSQFAGAAV
                     ELDAALLINPYDAAGIASALDRALDMPVQERRDRHGAMLRVMRENSLERWRDRFTGDL
                     AS"
     gene            complement(1371..1949)
                     /locus_tag="VFN42_07755"
     CDS             complement(1371..1949)
                     /locus_tag="VFN42_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996546.1"
                     /translation="MRLRHVTILALAGAVLAVPACADQISDEISKAQAAYQEHRPDIA
                     LAALDAAAASLRQQRADLLKSLLPPPPAHWTADPPETTAIGASTLGGGTSASCVYHLG
                     DQQVQVQFTTDSPMLQQMAELADTQLGQSPDVEIVMVDGQRATYSVHDNGFMSLVGAV
                     IVKVDGNAQVPKSTLRMFLDAVDFPTLQKLAH"
     gene            complement(2012..>2937)
                     /locus_tag="VFN42_07760"
     CDS             complement(2012..>2937)
                     /locus_tag="VFN42_07760"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8996547.1"
                     /translation="VRRLPTHGARGGRMQFDVLGTVLFAGFIAPVLLALERAQKFDPA
                     ALPGIGVLLAVAVVAALLLLRQERRAPSPLLPVQMFRNPGIWRADLMSACVAAQTVSM
                     VTFLPMYLQIVRGQNPAEAGLLLLPLTVGIAIGSLFAGKAIARTGRTAIMPSIGLAVT
                     AAGLVTLALFGPLLSNTQLMLLLAVIAAATGPTMPVVQITVQVVAGPQLLGAAAASVQ
                     FARSVGAAFGTAIVGAVLFALLAMRDPGLAHSFTALVEQGPRVLAGLPAAHRRLVQNE
                     IAEAFRGAFLMIATFTTGGLLMAWWLPVRRV"
BASE COUNT          495 a         1042 c          972 g          428 t
ORIGIN      
        1 cccgatctcg ctgttcacca tcgacctgtc ccgccgcgac tacgacgagt tctaccgcgg
       61 cttcgccaac gacacgctgt ggccggtgct gcattaccag atcggcctgc acagtttcga
      121 atggccgcag ttcgcagcct accgccgtgt caacgcgctg ttcgcgcaca gcctcgcgcc
      181 gctggtgcag ccggacgacg tgatctgggc gcacgactac cacctgctgt gcctggccga
      241 tgcgttgcgg gcagcggggc tgcgcaaccg catcggcctg ttcctgcaca cgccgttccc
      301 ggcgcccgcg gtgttcatga ccatccccgc gcacgccgag ctgatccgcg ccatgtgcgc
      361 ctacgacctg ctcggtttcc agaccgaggc cgaccgcacc gccttcaccg actatctgcg
      421 ccgccatgcc ggcggcgagg accgcggcgg cggcacgctg cgcgtattcg atcgcatcct
      481 gcgcaccggc gtgtatccga tcggcgtgca tatcgatgaa gtgcgcgccc aggccgaagc
      541 gccggccaac cgccgcaccg ccacgcgcat gcgcgccagc gtgctggaga agctgatcct
      601 cagcgtggac cggctggatt attccaaggg tctgcgccag cgcttcggcg ccttcgaacg
      661 cttcctgcaa gaccatccga aatggcaggg cagcgtgacg ctgatgcaga tcgcaccgcc
      721 gtcgcgcggc gacatccaga cctacaagga aatccgccgc gagctggaag ccgaagccgg
      781 ccgcatcaac ggccgtttcg ccgaggtgga ctgggtgccg ctgcgctacc tgaaccgcgg
      841 ctttccgcgc gcggtgctga tgccgctgta cgcggaggcg caggtcggcc tggtgacccc
      901 gttgcgcgac ggcatgaacc tggtggcgaa ggaatatgtc gcggcgcagg acccggacga
      961 tccgggcgtg ctggtgctgt cgcagttcgc cggtgccgcg gtggaactgg acgccgcact
     1021 gctgatcaat ccctacgacg ccgcggggat tgcctcggcg ctggatcgcg cgctggacat
     1081 gcccgtccag gaacgccggg atcgccacgg cgcgatgctg cgtgtgatgc gcgagaacag
     1141 cctggagcgc tggcgcgacc ggttcaccgg cgacctggcc tcgtgatcac gacgtcagca
     1201 cgcgaagaaa actggaaccg cagatgaacg cggatgaacg ccgatagcag gtcgcgatcg
     1261 tggtgcggac tcccgccgaa ggcggaaatt gccgctcacg cctgaccggg agcatgcgac
     1321 tccgccgcga tgcgcgccac tgcatccgcg ttcacctgcg gttccaaatt ctaatgcgcc
     1381 agcttctgca gcgtggggaa atcgaccgcg tccaggaaca tgcgcaaggt cgatttcggg
     1441 acctgcgcgt tgccgtccac cttcacgatc accgcgccga ccagactcat gaagccgttg
     1501 tcgtgcaccg agtaggtggc ccgctggcca tcgaccatca cgatctctac gtcgggtgac
     1561 tggcccaact gggtatcggc cagctcggcc atttgctgca gcatcgggct gtcggtggtg
     1621 aattgcacct gaacctgctg gtcgcccagg tgatagacgc aggacgcgga cgtgccgcca
     1681 cccagcgtgg aagcgccaat agcggtggtt tccggcggat ccgccgtcca gtgtgcgggg
     1741 ggcggcggca gcaggctctt caacaggtcg gcgcgttgct gccgcaggct ggcggcggcc
     1801 gcatccagcg cggccagcgc aatatccggc cgatgctcct gataggcggc ctgggccttg
     1861 ctgatttcgt cgctgatctg gtcggcacag gccggaacgg cgagcaccgc gccggcaagg
     1921 gccaggattg tcacgtggcg cagtcgcatc agcgtccccc tcattggcgg gaccttaagg
     1981 gagacggagg aacccgttca agcggcgttg atcagacccg ccgcaccggc agccaccagg
     2041 ccatcagcag gccgccggtg gtgaaggtcg cgatcatcag gaacgcgccg cgaaacgcct
     2101 cggcgatttc gttctgcacc agccggcgat gcgccgcggg caggccggcc agcacgcgcg
     2161 gtccctgctc gaccagcgcc gtgaaggaat gtgccagccc gggatcgcgc atggcgagca
     2221 gcgcgaacag caccgcgccg acgatggccg tgccgaacgc cgcgccgacc gaccgcgcga
     2281 attgcaccga tgccgcggcg gcgccgagca attgcggccc cgcaaccacc tgcacggtga
     2341 tctgcaccac cggcatggtg gggccggtcg ccgcggcgat caccgccagc agcagcatga
     2401 gctgcgtgtt gctcagcagc ggtccgaaca gcgccagcgt caccagcccc gccgccgtca
     2461 ccgccaggcc gatcgacggc atgatcgcgg tccgcccggt gcgcgcgatc gccttgccgg
     2521 cgaacagcga gccgatggcg atgccgaccg tcagcggcag cagcagcaat ccggcttccg
     2581 ccgggttctg cccgcgcacg atctgcaagt acatcggcag gaaggtgacc atcgagacgg
     2641 tctgcgccgc gacgcaggcc gacatcaggt cggcgcgcca gatccctgga ttgcggaaca
     2701 tctgcaccgg cagcagcggc gacggcgcgc gcctctcctg gcgcagcagc agcaacgcgg
     2761 cgaccaccgc caccgccagc agcaccccga tgccgggcag cgccgccgga tcgaatttct
     2821 gcgcgcgttc cagcgccaac agcaccgggg cgatgaagcc ggcgaacagc acggtgccca
     2881 acacatcgaa ctgcatccgc ccaccgcgcg ccccatgcgt cggcagccgc cgcacca
//
LOCUS       DASSZH010000358         4617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1508890, whole genome shotgun sequence.
ACCESSION   DASSZH010000358 DASSZH010000000
VERSION     DASSZH010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508890"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="VFN42_07765"
     CDS             complement(<1..914)
                     /locus_tag="VFN42_07765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET8996548.1"
                     /translation="MPRDLLPSTTKLQPYWWDEVPRPMLPPAPLPAEADVVVIGSGYT
                     GLAAALQTARGGRSTVVLDAEDAGFGCSTRNGGQISTSIKPDLATLTRRHGETVARAI
                     LQEGRKSLAWTGSFIAEEKIDCDFRVCGRFHAAHNPARYEELARSIAAQPRGLEVDAQ
                     LVPRSEQHQELGTDAYHGGVVYTQHASVDPARYHQGLLDRVLQAGAVMVPHCAATALQ
                     RDGAALRVRSARGEIRARHVIVATNGYTGSLLPWLQRRVIPIGSYIIATDPLPPETMA
                     RLMPRDRIVSDTRKVVYYYRPSPDGRRIV"
     gene            complement(927..2555)
                     /locus_tag="VFN42_07770"
     CDS             complement(927..2555)
                     /locus_tag="VFN42_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HET8996549.1"
                     /translation="MEFDYIIVGAGSAGCVLARRLAEAGTASVLLLEAGGSDSWPSLR
                     VPIGYGRSFYNPRVNWMYRTEPDPMLDDRRGYWPRGRVLGGSSTINAMVYVRGLPGDF
                     DDWRDAGNPGWGWQDVLPYFRQLETFSGGDDPARGRSGPLHVADVSAECHPLCTNFLR
                     ACEEAGFPRTGDINGGQPEGVGYYQITTHRGLRESAARAFLHPIARHGNLRIERHARV
                     LRVTIAGMRAVGVTFRQGREDHQVTARREVLLAAGAINTPQLLQLSGVGPAALLRQYG
                     IPVALDLPAVGRHLQDHLCIDHLFRTSVSTLNQALGRASGQVLAALRYGLWRRGPLAL
                     SVNQAGGFVRSQRDQPRPDMQLYFSPLSYTRGTPGRRALMRPDPFPGVLLSAQPCRPR
                     SRGCIDIQSPDPLASPRIAANALSHSDDQDALLRAQHLLRRIAAAPAMREIIVEDLHP
                     GPHCSGDAALIADMRARASTVFHPVGTCRMGADPATSAVDPALRVHGIAGLRVVDASV
                     FPSVTSGNTNAPVMMVAEKAASLILATRATAAMP"
     gene            complement(2619..4091)
                     /locus_tag="VFN42_07775"
     CDS             complement(2619..4091)
                     /locus_tag="VFN42_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET8996550.1"
                     /translation="MIAIDRASPRPIGTQLYVALRDLILSGAVANGTRLPATRTLADE
                     IGVSRTTVIEAFDRLVADGIVTSRVGAGTVVSVSLDLERPSQMPALAATPCAPRLSQA
                     AQSAVDHLAERPRLPHAPRAFVTGLPAFDAFPMAQWSRIAARHWRGSRRQIMGYGPPL
                     GDPRLRRAIAAHLGANRGIACEPDQVVIVSGAQQAFLNIGTLLLDPGDPVWFENPGAI
                     GARNGLLSVGARLVPVPVDEDGIQVAEGLRRCPDFRLVFVTPSHQQPLGHIMSIERRL
                     ALLRAAHAAGAWIIEDDYDGEFCFGRRPVSTLKSIDHAGAVLYVGTFSKSLFPALRLG
                     FIVVPTALIDPLARISSAANSGVPSSLQGIVAEFMEEGHFATHIRRMRRIYEERHAAL
                     LHAAHDFAGLLEVRSSDSGLHTVGFLQGGLSEQKAALAAEARQIVVAPLARYSIEPVA
                     LKGFALGFGGVRPHEIGHGMRILGEALEQLATARRRSRAA"
     gene            4234..>4617
                     /locus_tag="VFN42_07780"
     CDS             4234..>4617
                     /locus_tag="VFN42_07780"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HET8996551.1"
                     /translation="MKIIRIETFCTEFIGLVRVTAEDGLQGWGQVAPYNADITCSIVH
                     RQIVPWALGWDCDDISGLIAIIPQREHKFPGSYLARAMAGLDTALWDLRGKRLGKSVC
                     ELLGGKPRRLTAYASSMKRDITPDDE"
BASE COUNT          825 a         1542 c         1522 g          728 t
ORIGIN      
        1 acgatgcggc gtccgtccgg cgacggtcgg tagtaataga cgacctttcg ggtgtcgctg
       61 acgatgcgat cgcgaggcat cagccgcgcc atcgtctccg gcggcaaggg atcggtcgcg
      121 atgatatagc tgccgatcgg aatcacgcgc cgctgcagcc agggcaacag actgccggta
      181 tagccattcg tcgcgacgat cacgtgcctg gcacggattt cgccacgagc cgagcgcacg
      241 cgcaatgcgg caccgtcgcg ctgcaaggcg gtggcggcgc aatgcggcac catcaccgcg
      301 ccggcttgca gcacccggtc cagcaggccc tggtgatatc gtgccggatc caccgaggca
      361 tgctgcgtgt aaaccacgcc gccatgatac gcgtccgtgc ccagttcctg gtgttgttcg
      421 gaacgcggca ccagttgcgc atccacctcc aggccgcgcg gctgagcagc gattgaccgg
      481 gccagttcct catagcgcgc cggattgtgc gcggcgtgga aacgcccaca cacacggaaa
      541 tcgcaatcga tcttttcctc ggcgatgaag cttcccgtcc aggcgagcga ctttcgcccc
      601 tcctgcagga tcgcccgcgc cacggtctcg ccgtgccgcc gcgtcagagt ggcgagatcc
      661 ggcttgatgc tggtcgaaat ctgcccgcca tttcgcgtgc tgcacccgaa gccggcgtct
      721 tcggcgtcca gtaccaccgt gctgcggccg ccgcgcgccg tctgcagggc agcggcgagt
      781 ccggtatagc cggagccgat gaccaccacg tccgcctccg ccggcagtgg cgctggcggg
      841 agcatcgggc gcggcacctc atcccaccaa tagggttgca gtttcgtcgt tgacggtagc
      901 agatcccgag gcatgtgccc gactccttac ggcattgccg ccgttgcacg tgtcgccaga
      961 atcagcgacg cagccttctc ggccaccatc atgaccggcg cattggtgtt cccggaggtg
     1021 acgctgggaa acaccgacgc gtcaaccacc cgcaatcccg cgatgccgtg cacccgcagc
     1081 gccggatcga ccgcactggt cgctggatcg gcgcccatgc gacaggttcc cacaggatgg
     1141 aacacggtcg aggcgcgggc gcgcatatcg gcgatcaggg ccgcatcgcc gctacagtgc
     1201 gggccgggat gcaggtcctc gacgataatc tcccgcatgg ccggcgcggc ggcaatccgg
     1261 cgcagcagat gctgcgcccg cagcaatgca tcctgatcgt ccgaatgcga cagcgcgttc
     1321 gcggcgatac ggggcgatgc cagcggatcg ggcgattgaa tgtcgatgca gccgcggctg
     1381 cgcgggcggc atggctgcgc gctgagcagc acccccggga acggatccgg ccgcatcagc
     1441 gcacgacgcc caggtgtgcc gcgcgtgtag ctaaggggcg aaaagtagag ctgcatgtcg
     1501 ggacgcggct gatcgcgctg gcttcgcacg aaaccgccag cctggttgac gctgagtgcg
     1561 agcggtccgc gccgccacag gccatagcgc agcgcggcca gcacctggcc cgacgcgcgg
     1621 cccagcgcct ggttcaatgt ggaaaccgat gtgcgaaaca gatggtcgat gcagagatgg
     1681 tcctgcaaat gcctgccgac agccggcagg tccagggcga ccggtatccc gtattgccgc
     1741 agcagcgcgg caggtccgac gcccgaaagc tgcagcagtt gcggggtatt gatcgcgccg
     1801 gcggcaagca gcacttcccg cctcgctgtc acctgatgat cctcccgtcc ctgccggaac
     1861 gtcacgccca ccgcgcgcat tccggcaata gttacgcgca gcacccgtgc gtggcgctcg
     1921 atgcggagat tcccgtgccg tgcgattgga tgcagaaacg cacgtgccgc cgattccctc
     1981 aggccgcgat gcgtcgtgat ctgatagtaa ccgaccccct ccggctggcc gccattgatg
     2041 tcgccggtac gcggaaagcc ggcttcttca caggcacgca ggaaattggt gcaaagcgga
     2101 tggcattcgg cggacacatc cgccacatgc aacgggccgc tgcggccacg agccgggtcg
     2161 tcaccgccgg agaacgtctc cagctggcgg aaataaggca gcacgtcctg ccagccccaa
     2221 ccgggattgc cggcgtcgcg ccagtcatcg aaatccccgg gcaatccgcg gacatacacc
     2281 atggcgttga ttgtgctgga gccacccagc acgcgtcctc gcggccagta tccgcggcgg
     2341 tcatcgagca tcggatccgg ctcggtccgg tacatccagt tgacgcgggg gttgtagaac
     2401 gacctgccgt agccgatcgg cacgcgcagc gagggccagc tgtcgctgcc acccgcttcc
     2461 agcagcagca cgctggcggt tcccgcctcg gcaagccgcc gcgccaggac gcagcccgcc
     2521 gatcccgcgc cgacgatgat gtagtcaaac tccatggcaa cagcctgcgc cgtggctcat
     2581 gcggctgtca ggcccacttc cgacacgtta gcggtccatt atgccgcccg ggaacgccgg
     2641 cgggccgttg caagctgttc cagtgcctcg ccgaggatgc gcatgccatg gccgatctcg
     2701 tgtggacgca cgccgccgaa accgagcgcg aatcccttga gcgcgaccgg ttcgatcgaa
     2761 tagcgcgcca gcggggcgac gacgatctga cgggcttccg ccgccagggc ggccttctgt
     2821 tccgacaggc cgccctgcag aaatccgacg gtgtgcaacc cgctgtcgct ggacctgacc
     2881 tccagcaacc cggcgaaatc gtgcgcggcg tgcagcagcg cagcatggcg ttcttcatag
     2941 atgcgtcgca tccggcggat atgcgtggcg aaatgtccct cctccatgaa ctcggcgaca
     3001 atgccttgca ggctggacgg cacgccggaa ttcgccgcgc tggagatgcg cgccagtgga
     3061 tcgatcaatg ccgtcggcac gacgatgaag cccaatcgca gggcggggaa cagtgatttg
     3121 ctgaaggtgc cgacatagag cactgcaccg gcgtgatcga tgctcttcag cgtggacacg
     3181 ggccggcggc cgaagcagaa ctcgccgtcg taatcgtctt cgatgatcca ggcgccggcc
     3241 gcatgtgcgg cacgcagcaa cgcaagccga cgctcgatgg acatgatatg gccaagcggc
     3301 tgctgatgcg acggggtgac gaagaccaga cggaaatcgg gacagcggcg caggccttcc
     3361 gcgacctgga tcccgtcttc gtccaccggc acgggaacca gccgcgcacc gaccgagagc
     3421 agaccgttgc gcgcaccgat tgccccggga ttctcgaacc ataccggatc gccgggatcg
     3481 agcagcagcg tgccgatatt gaggaaggcc tgttgtgcgc cgctgacgat caccacctgg
     3541 tctggttcgc aggcaatccc ccgattggcg cccagatgcg cggcgattgc cctgcgcagc
     3601 cggggatcgc ccaatggcgg cccgtagccc atgatctgcc gccgcgaacc gcgccaatga
     3661 cgggcggcaa tgcgtgacca ctgagccatc ggaaatgcat cgaaggcggg cagccccgtg
     3721 acgaaggcgc gtggcgcatg cggcagacgc gggcgttcgg caagatggtc aaccgcggat
     3781 tgcgcggcct gcgacaaccg cggcgcacac ggtgtcgcgg cgagtgccgg catttgcgac
     3841 gggcgctcga ggtcgagcga gacactcacg accgtgcccg caccgacgcg ggatgtgacg
     3901 ataccgtcgg cgacgagccg gtcgaaggcc tcgatcaccg tggtgcgcga gacgccaatt
     3961 tcgtcggcaa gcgtgcgggt cgccggcagc cgcgtgccat tggcgacggc gccggacagg
     4021 atcaggtcgc gcagcgcgac gtagagttgc gtgccgatcg gtcgcggcga tgcgcgatcg
     4081 atcgcgatca tcggcagaag cgctcccccg ggtcgtttca cctgatccct gcccgccaac
     4141 gtattggtct tgtagtctag ccacaatgga ccttcgggag gaagccgctt tcacgcagat
     4201 tacaacggca atgacaggtg tgcttccgcg ccaatgaaga ttatccgcat cgagaccttc
     4261 tgcacggagt tcatcggcct ggtccgggtc accgcggaag acggcctgca gggttggggc
     4321 caggtggcgc cgtacaatgc ggatatcacc tgctcgatcg tgcatcgcca gatcgttcct
     4381 tgggcactcg gctgggattg cgacgatatt tccgggctga tcgcgatcat accgcagcgc
     4441 gagcacaaat ttcccggctc ctatctggcg cgggccatgg ccggcctgga cacggcgttg
     4501 tgggatctgc gcggcaagcg cctgggcaaa agcgtctgcg agctgctggg aggcaagccg
     4561 cgccggctga ccgcctatgc atcctcgatg aagcgcgaca tcacgccgga cgatgaa
//
LOCUS       DASSZH010000359         3940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_196212, whole genome shotgun sequence.
ACCESSION   DASSZH010000359 DASSZH010000000
VERSION     DASSZH010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196212"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VFN42_07785"
     CDS             <1..460
                     /locus_tag="VFN42_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HET8996552.1"
                     /translation="LRTSPVVTATPHFTPATIIAGMQAMPGAQHLNRETRAVHAAAFW
                     HPARGLLALREDVGRHNALDKLAGAMARQQIDPGEGLLLLSSRLSVELIQKAAMIGLP
                     AIVAVSVPTALAVRTAEAAGITLIGIARGDTFEVFTRGDRIATHKVQHVA"
     gene            474..671
                     /locus_tag="VFN42_07790"
     CDS             474..671
                     /locus_tag="VFN42_07790"
                     /inference="COORDINATES: protein motif:HMM:NF022832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HET8996553.1"
                     /translation="MANQIGKFFAHQKHDAAVAAIEDHIRRFWDPRMRRTIIAGLDSP
                     DLRLDPTVREAIAKLQESQVA"
     gene            819..2135
                     /locus_tag="VFN42_07795"
     CDS             819..2135
                     /locus_tag="VFN42_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HET8996554.1"
                     /translation="MVPPAALCVHLCIGQVYAFSVFNLPMTKLIGITKSVPGDWKLTD
                     LGWIFSLAILVLGLAAASLGRWVEESGPRKAMVVSGLCWGGGFLVAALGVYMHNIWVV
                     YFGYGVLGGIGLGLAYISPVSTLIKWFPDRPGMATGMAIMGFGGGAFIASPLSVWLMK
                     HFSTPTHVGVAETFVVLGVVYFCFMMVGAAIVRLPAPGWAPAGYVAPAQPKKLITTRN
                     VFVYQALKTPQFWLIWWVLCLNVTAGIGVLGQASAMSQEMFPGRVTVVAAAGFVGLMS
                     LFNMGGRFFWASVSDFIGRKNTYSVFFILGFVLYASIPYVAAAGSVAGFVLFFCVIIS
                     MYGGGFSTVPAYLKDMFGTRYVGAIHGWLLTAWSAAGVFGPVLVNYIRQYEVTHGVPK
                     ADAYNYTMYIMAGLLIVGLICNLFISAVHERHHMGEESEPLAATAD"
     gene            2148..2273
                     /locus_tag="VFN42_07800"
     CDS             2148..2273
                     /locus_tag="VFN42_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996555.1"
                     /translation="MPATTQDQTTPLHLILAWGFVGIPLAWGVFQTVLNALKLFH"
     gene            complement(2326..2835)
                     /gene="tsaA"
                     /locus_tag="VFN42_07805"
     CDS             complement(2326..2835)
                     /gene="tsaA"
                     /locus_tag="VFN42_07805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HET8996556.1"
                     /translation="MAQGPDAMAAGFTIRPIGTLRTPWHSVADCPRNGRQPDPAPLCH
                     AEIDPEFVAGLRGLDGFSHLILLYWLDQAKPAELVFTPPFDDQPRGVFTTRAPRRPNP
                     IGLAVVAFDGFDTPDRLALRYLDCVDGTPLLDIKPYLPTTDSEPQARMGWLQPHATRN
                     RNTAAEGGR"
     gene            complement(2909..3358)
                     /locus_tag="VFN42_07810"
     CDS             complement(2909..3358)
                     /locus_tag="VFN42_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006557114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HET8996557.1"
                     /translation="MPMAQVFSQFGCTGKNISPQLSWSGEPAGTKSFAVTMYDPDAPT
                     GSGWWHWTVFNIPANVHSLPENAGAAHGTLPAGATEGRTDFGFSHYGGPCPPAGDHPH
                     RYVITVFAVKVAKLPLDAQSSGALVGFNLHFNTLAKTHMTAHYGRKH"
     gene            3604..>3940
                     /locus_tag="VFN42_07815"
     CDS             3604..>3940
                     /locus_tag="VFN42_07815"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HET8996558.1"
                     /translation="MGRTSKQVASFAAAVQDVADGSTIGFGGFAMPGVPFNLIQALLE
                     QGAKNLTLVANTTGGAQQPRMQDIGMLVENGQVKKVICAFTASTRPTDVIPFTKYYES
                     GEVDAELVPQ"
BASE COUNT          626 a         1317 c         1289 g          708 t
ORIGIN      
        1 gctgcgcacc tcgccggtgg tgaccgccac accgcatttc acccccgcca ccatcatcgc
       61 cggcatgcag gcaatgcccg gcgcgcagca cctcaaccgc gagacgcgcg cggtgcatgc
      121 ggcggccttc tggcatcccg cgcgggggct gctcgccttg cgtgaggatg tcggccggca
      181 taacgcactc gacaagctgg ccggcgccat ggcgcggcaa cagatcgacc ccggcgaagg
      241 actgctgctg ctgtccagcc gcctgtcggt cgagctgatc cagaaagccg cgatgatcgg
      301 cctgcccgcc atcgtcgcgg tctccgtccc gaccgcgctc gccgtccgca ccgcggaggc
      361 cgccggcatc accctgatcg gcatcgcccg tggcgacacc ttcgaggtgt tcacccgcgg
      421 cgaccgcatc gccacacaca aggtgcagca tgtcgcctga caaactcgcg tatatggcca
      481 accagatcgg caagttcttt gcccaccaga agcacgacgc ggcggtggcc gccatcgagg
      541 atcatatccg ccgcttctgg gatccgcgca tgcggcggac catcatcgcg ggtctggaca
      601 gtccggatct tcggcttgat ccgacggtgc gggaagcgat tgccaaactt caggaatctc
      661 aagtagcata acgaagccga tccaattcgg cgtctgcgaa ctatcaaaaa agaacttggg
      721 agcgtctaat gagttcgacc gccatagggg gcggcggtgt ctcgcacgga atgttttcgc
      781 gcgagcgcat tattgcccgc cccggattca gccgctggat ggtaccgccg gccgcgctgt
      841 gcgtacatct gtgtatcggc caggtctatg cgttcagcgt gttcaacctg ccgatgacca
      901 agctgatcgg cattaccaaa tccgtccccg gtgactggaa gctcaccgac ctgggctgga
      961 tcttctccct cgccatcctg gtcctcggcc tcgccgctgc atcgcttggc cggtgggtgg
     1021 aggaaagcgg gccgcgcaag gcgatggtcg tctccggcct gtgctggggc ggcggattcc
     1081 tggtcgccgc actcggcgtc tatatgcaca acatctgggt cgtctatttc ggctacggcg
     1141 tacttggcgg catcggcctg ggactggctt atatctcgcc cgtctccacc ctgatcaaat
     1201 ggttcccgga cagaccgggc atggccaccg gcatggcgat catgggtttt ggcggcggcg
     1261 cgttcattgc gtcgccgctt tccgtttggc tgatgaagca tttctccacg ccgacccatg
     1321 tcggtgtcgc cgaaaccttc gtcgtgctcg gcgtggtgta tttctgcttc atgatggtgg
     1381 gcgcggcgat cgttcgcctg ccggccccgg gctgggcgcc cgccggctat gtcgcgccgg
     1441 cacagccgaa gaagctgatc accacgcgca acgtgttcgt ctaccaggcg ctgaagacgc
     1501 cgcagttctg gctgatctgg tgggtgctgt gcctgaacgt caccgccggc atcggcgtgc
     1561 tgggccaggc ctccgcaatg agccaggaga tgttccccgg ccgcgtcacc gtggttgccg
     1621 ccgccggctt cgtcgggctg atgagcctgt tcaacatggg cggccggttc ttctgggcgt
     1681 cggtgtccga cttcatcggc cgcaagaaca cctactcggt gttcttcatc ctgggcttcg
     1741 tgctgtacgc gtcgattccc tatgtcgcgg cggccggcag cgtcgccggc ttcgtgctgt
     1801 tcttctgcgt catcatcagc atgtatggcg gcggcttctc caccgtgccg gcctatctga
     1861 aggacatgtt cggcacgcgc tatgtcggcg ccatccatgg ctggctgctc accgcatggt
     1921 cggccgccgg cgtgttcgga cccgtgctgg tgaactacat ccgccagtac gaggtgacgc
     1981 acggcgtgcc gaaagccgat gcctacaact acaccatgta catcatggcc ggactgctga
     2041 tcgtcggtct gatctgcaac ctgttcatca gtgcggtgca cgagcggcat cacatgggcg
     2101 aggaatccga acccctcgcc gcgaccgccg attgagggga ggcaaccatg cccgcgacaa
     2161 cacaagacca gaccacgccg ctgcacctga tcctggcctg gggcttcgtc ggcatcccgc
     2221 ttgcctgggg ggtgttccaa acggtgctga acgcgttgaa gctgttccac tgaccgcgcc
     2281 cgtccctctc cgcccggctc ggcggagagg gatcggcttc cgggcttagc ggccgccctc
     2341 cgcggccgtg ttgcgattgc gcgtcgcatg cggctgcagc catcccatgc gcgcctgcgg
     2401 ttcgctgtcg gtggtgggca gatacggctt gatatccagc agcggtgtgc cgtccacgca
     2461 gtccaggtaa cgcagcgcca gccggtccgg cgtgtcgaac ccatcgaatg ccaccaccgc
     2521 aagcccgatc ggattgggcc ggcgtggcgc gcgggtggtg aacacgccgc gcggctggtc
     2581 atcgaacggc ggggtgaaca ccagctccgc cggcttcgcc tggtccagcc agtacagcag
     2641 gatcaggtgg gagaacccat ccagcccgcg cagccctgcc acgaattccg ggtcgatctc
     2701 cgcatgacac agcggcgccg gatcgggctg gcggccgttg cgcgggcaat ccgcgacgct
     2761 gtgccagggc gtgcggagcg tgccgatcgg tcgaatggtg aagcctgcgg ccatggcgtc
     2821 cggtccttgt gccatgcgcg ccagcatagc ctccggcgcg cggaatgcga ccgccggcac
     2881 aaggaccagc cggaacgcgc cgtcagcctc agtgcttgcg gccgtaatgc gcggtcatgt
     2941 gcgttttggc gagcgtgttg aagtgcaggt tgaaccccac cagtgcgccg gatgactgcg
     3001 catccagcgg cagcttcgcc accttcaccg cgaagacggt gatgacatag cgatgcggat
     3061 ggtcgccggc gggcggacac ggcccgccgt aatgcgagaa gccgaaatcc gtgcggccct
     3121 cggtggcgcc ggccggcagg gtgccatgcg ccgcgcccgc gttctccggc aggctgtgca
     3181 cgttcgccgg gatgttgaag acggtccaat gccaccagcc gctgccggtc ggcgcgtccg
     3241 gatcgtacat ggtgaccgcg aagctcttgg tgccggcggg ttcacccgac caggacagtt
     3301 gcggcgagat gttcttgccg gtgcagccga actggctgaa cacctgtgcc atcggcatgg
     3361 tcccgccgtt atggacgtct ttgctggtaa cggtgaatcc ggcccccatc gcggtcgccg
     3421 ccggcaggca gaggcacaag gccaggacag gcgcgcgcat cgattttccc cccacgattg
     3481 ttccgggcat ttagccatgc accggcgcgg cgcggcaagc aggaaccgcc gccgccggcc
     3541 cgcttgcttg acacggctgt ggcgccgtcg gttcatcgcc ctggcaaacc ggacggaacg
     3601 gcgatggggc ggacatccaa gcaggtggcg agcttcgcgg cggcggtgca ggacgtcgcc
     3661 gacggcagca ccatcgggtt cggcggcttc gccatgccgg gcgtgccgtt caacctgatc
     3721 caggcgttgc tggagcaggg cgcgaagaac ctgaccctgg tggccaacac caccggcggc
     3781 gcgcagcagc cgcgcatgca ggatatcggc atgctggtgg agaacggcca ggtgaagaag
     3841 gtgatctgcg ccttcaccgc atcgacgcgc cccaccgacg tgatcccctt caccaaatac
     3901 tacgagtcgg gcgaagtcga cgccgaactc gttccgcagg
//
LOCUS       DASSZH010000360         2569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1338198, whole genome shotgun sequence.
ACCESSION   DASSZH010000360 DASSZH010000000
VERSION     DASSZH010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338198"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VFN42_07820"
     CDS             <1..574
                     /locus_tag="VFN42_07820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HET8996559.1"
                     /translation="ISAEWRQKQIEYTWVRSLAGKAHHRDFWHLTEEALAWAAAKHGI
                     GDAALLAEVLQAYRRLTVYPEAKPMLERLRAMGIGRAILSNGEPGMLADGVQSAGIQG
                     LLDDVLSAESAGVFKPDPRVYRLATDRFGLSAGQMAFVSSNPWDAFGASAFGFQVFWV
                     NRHKQPAEYDLHRRATELPDLSALADRLAG"
     gene            893..1966
                     /locus_tag="VFN42_07825"
     CDS             893..1966
                     /locus_tag="VFN42_07825"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HET8996560.1"
                     /translation="MTAPLLEVTDLSRHFAVKRGLVLTRTVGAVRAVDGISFTLGRGE
                     TLALVGESGCGKSTTARMVLRLIDPSTGSIRFEGTDITTLTGEALRKLRRRLQIVFQD
                     PFASLNPRMTVGDILEEPLIVHGIGDRTSRQARVKELLSLVGLAAFHAQRYPHEFSGG
                     QRQRIGIARALAVEPALIVCDEPVSALDVSIQAQVINLLKDLQTRLGLSYLFIAHDLA
                     VVKHTANRIAVMYLGQIVEIARKDALFADPRHPYTRTLLAAIPRPDPHRDRHRPVAGG
                     DVPSPMNPPPGCRFHTRCPFAIDRCRTEMPVLRPWGEGHLTACHRAEDLPPLDETAAG
                     HMAPAAAKRLALYARRRADVAAG"
     gene            2150..>2569
                     /locus_tag="VFN42_07830"
     CDS             2150..>2569
                     /locus_tag="VFN42_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593986.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HET8996561.1"
                     /translation="MSESHNGQQGQCPVVHGARPKAARANRDWWPDQLNIDMLHRSSS
                     LSDPMGSGFDYAREFRSLDLHAVIADLTALMTDSQDWWPADWGHYGGLMIRMAWHSAG
                     TYRISDGRGGAGHGTQRFAPLNSWPDNANLDKARLLLW"
BASE COUNT          402 a          884 c          879 g          404 t
ORIGIN      
        1 gatcagtgcc gagtggcggc agaagcagat cgagtacacc tgggtccgca gcttggccgg
       61 caaggcgcat caccgcgatt tctggcacct gaccgaggag gcgctggcct gggcggcagc
      121 gaagcatggc atcggcgatg cggcgctgct ggccgaggtg ctgcaagcct atcgccgcct
      181 gaccgtctat cccgaggcga agccgatgct ggagcggctg cgggcgatgg ggatcggccg
      241 ggccatcctg tcgaacggcg aaccgggcat gctggccgac ggcgtgcagt ccgccgggat
      301 ccaaggcttg ctggatgacg tgctcagcgc ggaatccgcc ggcgtgttca agccggatcc
      361 gcgcgtctac cggctggcga ccgaccggtt cggcctgtcc gccgggcaga tggcgtttgt
      421 ctcgtccaat ccctgggacg cgttcggcgc cagcgcgttc gggtttcagg tgttctgggt
      481 gaaccgccac aagcagccgg cggagtacga cctgcaccgc cgggccaccg agttgcccga
      541 cctgtcggcg ctggcggaca ggctggcggg gtagtgtgtg ccgccggcgg gcgcagcctg
      601 ggcgttaaag tagccgccca gaccccgtaa ggattcgtgg agaaataagt gctgcgtttg
      661 gcgtcgcggc cattcgtacc tcgtcatggc gggcgaaggc ccgccatcca cgacttgctc
      721 gctccgtgct gcaaagtcgt ggatggtgcg ccttcgcgca ccacgacgcc gaagcgcctg
      781 ggtgccgctg cgacgattcg tttatttccc tacgagccct tcgcccgtca cggcgtgggc
      841 ggcattgtcg tcctgccagc ccaccgctgc gtcgtccccg cggggtgctc cggtgaccgc
      901 accactgctc gaagtcaccg atctgtccag gcatttcgcg gtcaagcgcg ggctggtgct
      961 gacccgcacc gttggtgcgg tgcgcgcggt ggacggcatc tccttcacgc tcgggcgcgg
     1021 ggagacgttg gctctggtcg gtgagtccgg ctgcggcaaa tccaccaccg cgcgcatggt
     1081 gctgcggctg atcgatccgt ccaccggcag catccgcttc gaaggcaccg acatcacgac
     1141 gctcaccggt gaggctctgc gcaagctgcg gcgccggttg cagatcgtgt tccaggaccc
     1201 gttcgcctcg ctcaacccgc gcatgacggt gggcgacatc ctggaagagc cgctgatcgt
     1261 gcatggcatc ggcgaccgca cgtcgcgcca ggcacgggtc aaggagttgc tgagcctggt
     1321 cgggctcgcc gcgttccatg cccagcgcta tccgcacgaa ttctccggcg gacagcgcca
     1381 gcgcatcggc atcgcccgcg cgctcgcggt ggagccggcg ctgatcgtgt gcgacgaacc
     1441 cgtgtccgca ctggacgtgt cgatccaggc gcaggtgatc aacctgctga aggacctgca
     1501 gacgcggctc ggcctgtcct atctgttcat cgcgcacgac ctggcggtgg tgaagcacac
     1561 cgccaaccgc atcgcggtga tgtatctcgg ccagattgtg gagatcgcca ggaaggacgc
     1621 gctgttcgcc gatccgcggc acccgtacac gcgcaccctg ctggcggcga tcccgcggcc
     1681 cgatccgcat cgcgaccggc atcgcccggt ggccggcggc gacgtgccca gcccgatgaa
     1741 tccgccgcca ggctgtcgtt tccacacgcg gtgccccttc gccatcgacc gctgccgcac
     1801 cgagatgccg gtgctgcggc cctggggcga gggccacctg accgcctgcc atcgcgccga
     1861 ggatctgccg ccgctggatg agaccgccgc cggtcatatg gcgccggccg cggcgaagcg
     1921 gctggcgctg tacgccaggc gccgagctga cgttgcggcg ggctgaccgc ggccgacctg
     1981 atcatcgaac gcttcaatcg tctcgatcag attaatcagt tttaccgcta accgccggac
     2041 cggcatagac caaccggacg aggcgcaaga gcaaggggcc agcccgggtg accgggagca
     2101 cccgccgccg gactgcgaca cccagatcca accgtgaagg gagcggatga tgagcgaaag
     2161 tcacaacgga cagcagggcc agtgcccggt ggtgcacggc gcccggccga aagccgcccg
     2221 cgccaaccgt gactggtggc cggaccagct caacatcgac atgctgcatc gcagctccag
     2281 cctgtccgac ccgatgggtt cggggttcga ctacgcgcgc gaattccgca gcctggacct
     2341 gcacgccgtc atcgccgacc tgaccgcgct aatgaccgac agccaggatt ggtggccggc
     2401 cgactggggc cattacggcg ggctgatgat ccgcatggcc tggcacagcg ccggcaccta
     2461 tcgcatcagc gacggccgtg gcggcgccgg gcacggcacc cagcgcttcg ccccgctgaa
     2521 ctcctggccg gacaacgcca acctggacaa ggcacggctg ctgctgtgg
//
LOCUS       DASSZH010000361         5001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_792862, whole genome shotgun sequence.
ACCESSION   DASSZH010000361 DASSZH010000000
VERSION     DASSZH010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5001
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792862"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VFN42_07835"
     CDS             complement(<1..610)
                     /locus_tag="VFN42_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8996562.1"
                     /translation="MTAIALNDVRLTLGGRTILDEVSLDFAEGEFVGVLGPNGAGKTT
                     LMRAILGLVPPEHGRILVLGQPATSGNPAIGYMPQVRGMAAPQLSGRDFVAGVVEGHR
                     LGLPILGRSGHAEVDRVLDLVGARDLARRKLGTLSGGERQRLLLAQALVGRPRLLLLD
                     EPLISLDPHRQTEVVALTRAVQQELGITLQFSAHELNPLLSAL"
     gene            complement(612..1472)
                     /locus_tag="VFN42_07840"
     CDS             complement(612..1472)
                     /locus_tag="VFN42_07840"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HET8996563.1"
                     /translation="MRLLPSLAALAVLLTPVLACATAVSVVAAENVYGDVARQIGGRD
                     VTVRSILQNPDQDPHLFEVTPSAARAVSAADILVLNGLGYDPWMATLLHATPRRGRIV
                     LDAGALAHRKTGDNPHIWYDTTVMLRAAAALAGALQQADPAHAAGYRQRLAVFRQAMQ
                     PVQARIAVLHARLAGLTVTATEPVFGDMLKALGMTSRNMGFQLAVMNNTEPSASQVAD
                     FETDLRQHRVRLLVYNSQAVDPIATHMRNLAVASHIPVVGVTETEPPGMTYQAWMLAE
                     LDAVARAVPD"
     gene            1589..2998
                     /locus_tag="VFN42_07845"
     CDS             1589..2998
                     /locus_tag="VFN42_07845"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HET8996564.1"
                     /translation="MRHCRVLALAAGALACASAPAAAQSADASGAPAAPSGFWDRATL
                     LGDIGGLRLALGAHGITLSLSETSEVLGNVTGGVHRGAAYDGETTMSLSLDTAQAFGW
                     TGGTLFISALQTHGRNLSADNLDTLQTASGIEAERTTRLWELWYDQAFLGGKLDVKLG
                     QISADQEFMFSQYSALYVNTVMGWPSLPSADLYAGGPAYPLSSLGVRVRAHANDAVTV
                     LAGVFDDNPPGGAFSDDSQLRGREASGTAFNRNTGALWLAEVQYAVHQSPAESCVRLS
                     CGLPGIYKLGAWYDSGRFPDQHFGTDGLSLANPASNGDPLMHRGDFSLYALADQMVWR
                     QAGGPRAVGVFARMIGAPANQNLVTSSLNAGVNVTAPLPGRGKDTLGIGFGWTQVSPR
                     AAALDRDTAGFSATAYPVRGSEEFIELTYQLQMAPWWIVQPDLQYVFNPGGGIPNPNN
                     PSHVVGNELVLGVRTVVTF"
     gene            3051..3581
                     /locus_tag="VFN42_07850"
     CDS             3051..3581
                     /locus_tag="VFN42_07850"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8996565.1"
                     /translation="MSRLFGDMRQVGIVVRDIEAAMRHWIEVCGVGPWFYTDRLAVTA
                     FTYAGKRYDEIHLSIALANSGDVQLELIQQRSDHPSMYRDFLAAGHEGMQHWSSWPAD
                     YEARYQRALASGYSVGQEGDSPRGRFVYFRDEGHPGTVIEMADATPNRMRIFGAVREA
                     ARDWDGSDPIRRQWPS"
     gene            complement(3598..4308)
                     /locus_tag="VFN42_07855"
     CDS             complement(3598..4308)
                     /locus_tag="VFN42_07855"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HET8996566.1"
                     /translation="MHARKVALHVEHHRVAISKHHANIRHVKHAIQSVHARHYTHVAG
                     ISCVPFARQDSGISLPGNAWAWWGNAAGVYSRGRVPQPGAVLAFRSNPHMPLGHVAVV
                     TKVINPREIEIDQANWVHGRITRGVPVVDVSEANDWSAVRVEMGHSGSFGSVYPTYGF
                     IYDKPDNGTVLAAAHAPAPQPVLNAAPSDLRPVAEQPWQTYEEVAELPTTPRHYVRPH
                     PVLTMQHAHAQSRVADRR"
     gene            4222..4437
                     /locus_tag="VFN42_07860"
     CDS             4222..4437
                     /locus_tag="VFN42_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996567.1"
                     /translation="MLNMPDVGMVFANRDAVMLDMQRHLAGMHDVLGADVPDKMRRYM
                     IFSSDVANGMMRCGLSGRNGQASCDGE"
     gene            complement(4763..>5001)
                     /locus_tag="VFN42_07865"
     CDS             complement(4763..>5001)
                     /locus_tag="VFN42_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003624223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET8996568.1"
                     /translation="GQTADVLAQIDDLLEQHGTDKTRLLQAQIWVKHISDRGAMNEVW
                     TPWLPPGGAPVRACVQAELASPELLVEIMVTACK"
BASE COUNT          796 a         1647 c         1742 g          816 t
ORIGIN      
        1 ccagcgcgct gagcagcggg ttgagctcgt gcgcgctgaa ctgcagggtg atcccgagtt
       61 cctgctgcac cgcgcgggtc agggccacga cctcggtctg gcggtgcggg tccaggctga
      121 tcagcggctc gtcgagcagc agcaggcgcg gccggccgac cagcgcctgg gccagcagca
      181 gccgctggcg ctcgccaccg gagagcgtgc caagcttccg ccgcgcgagg tcgcgcgcgc
      241 cgaccaagtc tagcacgcgg tcgacttccg cgtggccgga gcggccgagg atcggcaggc
      301 ccagccggtg cccctccacc acgccggcta cgaagtcgcg gccgctgagc tgcggcgccg
      361 ccatgccgcg cacctgcggc atgtagccaa tggctggatt gccgcttgtt gccggctgcc
      421 ccagcaccag gatgcggccg tgctccggcg gcaccaggcc gaggatggcg cgcatcagcg
      481 tggtcttgcc ggcgccgttg ggacccagca cgccgacgaa ttccccctcg gcgaagtcca
      541 ggctgacctc gtccaggatg gtccggccgc ccagggtcag gcggacatcg ttgagcgcga
      601 tggcggtcat actagtcggg cacggcccgg gcgaccgcgt ccagctccgc cagcatccag
      661 gcctggtagg tcatgcccgg cggctcggtt tcggtgacgc ccaccaccgg gatatgcgac
      721 gccacggcca ggttccgcat gtgggtggcg atcggatcga cggcctggct gttgtagacc
      781 agcaggcgca cgcggtgctg ccgcaggtcg gtctcgaaat cggcgacctg ggacgcgctg
      841 ggttcggtgt tgttcatcac cgcaagctgg aaacccatgt tgcgcgaggt catgccgagc
      901 gccttcagca tgtcgccgaa caccggctcg gtcgcggtca ctgtcaggcc ggccaggcgg
      961 gcatgcagca ccgcaatccg cgcctgcacc ggctgcatcg cttggcggaa caccgccagc
     1021 cgctggcggt atcccgcggc atgggccggg tccgcttgct gcaacgcacc ggccagcgcc
     1081 gcggcggccc gcagcatcac cgtggtgtcg taccagatat gcgggttatc gccggtcttg
     1141 cggtgcgcca gagcgccggc atcgaggacg atccggccgc gccgcggcgt cgcatgcaac
     1201 agcgtggcca tccagggatc gtagcccagg ccgttgagca ccaggatatc ggcagcggag
     1261 accgcgcgcg ctgcggacgg ggtgacttcg aacaggtgcg ggtcctggtc cgggttttgc
     1321 aggatgctgc gcaccgtcac gtcccgtccg ccgatctgcc gggccacgtc gccatagacg
     1381 ttctcggcgg cgacaacgct gaccgcggtg gcgcaggcca ggaccggcgt aagcagaacg
     1441 gccagggccg ccaggcttgg cagaagacgc atgaaggaac tctgggacag gggccgcgca
     1501 atcgggtgtt ataatgtaac ctattctgcc gtccagactg gcgaagcgag acggcgggtc
     1561 catcttcgcc ctgaccgagg agttgatcat gcgtcactgt cgggttctcg ccctggcggc
     1621 gggagcgctt gcctgcgcct cggcgccagc agcggcccag agtgccgatg cgagcggggc
     1681 gccggctgct ccgtccggct tctgggaccg cgcgacgctg ctcggcgaca taggcgggct
     1741 gcggctggcc ctgggcgcgc acggcatcac cctgtccctg tcggaaacca gcgaggtgct
     1801 cggcaacgtc accggcggag tgcatcgcgg cgccgcctat gacggcgaga ccaccatgag
     1861 cctgagcctg gatacggcgc aggccttcgg ctggacgggc ggcacgctgt tcatcagcgc
     1921 gctgcagacc cacggacgca acctcagcgc ggacaatctc gacacactgc agaccgccag
     1981 cgggatcgaa gcggaacgca ccacgcggct gtgggagctg tggtacgacc aggcctttct
     2041 gggcggcaag ctggacgtga agctcgggca gatcagcgcc gaccaggagt tcatgttcag
     2101 ccagtactcc gcgctgtacg tgaacacggt gatgggctgg ccgagccttc cctcggccga
     2161 tctgtacgcc ggcggcccgg cctatccgct gtcgtcgctc ggggtacggg tccgcgccca
     2221 tgccaatgac gcggtgacgg tgcttgccgg ggtgttcgac gacaacccgc cgggcggcgc
     2281 gttttccgat gactcgcagt tgcgggggcg ggaggcgtcg ggcaccgcgt tcaaccgcaa
     2341 caccggggcc ttgtggctcg ccgaagtgca gtatgcggtg caccagtcgc cggcggaatc
     2401 gtgcgtccgc ctgtcctgcg gcttgcccgg catctacaag ctcggtgcct ggtacgacag
     2461 cggccgtttt ccggaccaac acttcggcac ggacgggctg tcgctcgcca atccggccag
     2521 caatggcgat ccgctgatgc accgcggcga tttcagcctc tatgcgctgg ccgaccagat
     2581 ggtgtggcgg caggcgggcg ggccgcgagc ggtcggcgtg ttcgcgcgga tgattggggc
     2641 gcctgccaac cagaacctgg tgacctcgag cctgaatgcc ggcgtgaacg tgacggcgcc
     2701 gctgccgggc cgcggcaagg acacgctggg gattggcttc ggctggacgc aggtcagccc
     2761 gcgcgctgcc gcgctggatc gcgacacggc cggcttcagc gccacggcct atccggtgcg
     2821 ggggagcgag gaattcatcg aactgaccta tcagcttcag atggcgccct ggtggatcgt
     2881 gcagcccgac ctgcaatacg tgttcaatcc cggcggcggc attcccaatc cgaacaaccc
     2941 gtctcatgtg gtcggcaacg aactggtgct gggggtgcgc accgtagtta cgttctgagc
     3001 cggatcgcct gctatgctgt ccccgaccga accggtcggg gagaaacgtc atgagtcgcc
     3061 tgtttggaga catgcgccag gtcggcatcg tcgtgcgcga catcgaggcc gccatgcggc
     3121 attggatcga ggtctgcggc gtcggtccgt ggttctacac cgaccgcctc gccgtgacgg
     3181 ccttcactta tgccgggaag cgctacgacg agatccacct gtcgatcgcg ctggccaatt
     3241 ccggcgacgt gcagttggaa ctgatccagc aacgcagcga ccaccccagc atgtaccgcg
     3301 acttcctggc ggccggccac gaggggatgc agcactggtc aagctggccg gccgactatg
     3361 aggcacgcta ccagcgggcg ctggccagcg gctacagcgt gggacaggaa ggcgacagcc
     3421 cgcgcgggcg cttcgtgtat ttccgcgacg aaggccaccc gggcacggtg atcgagatgg
     3481 cggatgccac gccgaaccgc atgcgcatct tcggcgcggt gcgcgaggcg gcgcgcgact
     3541 gggatggcag cgatccgatc cgccggcagt ggccgagctg agccgccgcc cgcggcccta
     3601 tctgcggtcc gccacccgac tctgcgcatg cgcgtgctgc atcgtcagca ccggatgcgg
     3661 ccggacatag tgccgcgggg tggtgggcaa ctcggcgact tcctcatagg tctgccacgg
     3721 ctgctcggcg accgggcgca ggtcgctggg cgcggcgttc agcaccggct gcggtgccgg
     3781 cgcatgcgcc gccgccagga ccgtgccgtt gtccggcttg tcgtagatga acccataggt
     3841 cgggtacacg ctgccgaacg agccgctatg gcccatttcc acgcggaccg cgctccagtc
     3901 gttcgcctcg gacacgtcca cgacggggac gccgcgggtg atccggccat gcacccaatt
     3961 ggcctggtcg atctcgattt cccgcgggtt gatgactttt gtgacgacgg cgacgtggcc
     4021 gagcggcata tgggggttgg agcggaaggc cagcacagct cccggttgtg gaacacggcc
     4081 gcggctgtag acgccggcgg cgttgcccca ccaggcccag gcattgcccg gcagggagat
     4141 tccggagtcc tggcgggcga agggcacgca ggaaatgccg gcgacatgcg tgtaatgccg
     4201 cgcatggacg gattggattg catgcttaac atgccggatg ttggcatggt gtttgctaat
     4261 cgcgacgcgg tgatgctcga catgcagcgc caccttgcgg gcatgcacga tgtgcttggc
     4321 gcggacgtgc cggacaaaat gcgccgttac atgatttttt cgagcgacgt ggcgaatggt
     4381 atgatgcgtt gcggcctcag cggaaggaac ggtcaagcca gttgcgatgg tgaatagagt
     4441 gcccagaatc agggtcgtga ccgcgaaact tgcctcgcgc atcgatatat ccccgcgttt
     4501 ttgccccaga cgtgagcggc ggtagcagca ggtttgcgtc tatggcaacc cagtgacata
     4561 acgattgtgg cttcagagcc gcagttcgtt cgaaatcacc tgtggataaa gcgtaatgcc
     4621 gggaacaaac tcctaaatcg ccaacgtttg atgccgatgc gagagggcgg gggtcgggac
     4681 tcccgcctca cttcacgaac ccgttagcac cgttccgcaa caccaacccc gccgcgagtc
     4741 gcaacggggt tggctgctac cgtcacttgc aggccgtcac catgatctca accagcagct
     4801 ccgggctggc cagctcggcc tgcacgcacg cccgcaccgg agcgccgccg ggcggcagcc
     4861 agggggtcca cacctcgttc atggcgccgc ggtcgctgat gtgcttcacc cagatctgcg
     4921 cctgcaacag ccgggtcttg tcggtgccgt gctgttccag caggtcgtcg atctgcgcca
     4981 gcacgtccgc ggtctggcct t
//
LOCUS       DASSZH010000362         6868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1372411, whole genome shotgun sequence.
ACCESSION   DASSZH010000362 DASSZH010000000
VERSION     DASSZH010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6868
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372411"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..82)
                     /gene="rpmF"
                     /locus_tag="VFN42_07870"
     CDS             complement(<1..82)
                     /gene="rpmF"
                     /locus_tag="VFN42_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET8996569.1"
                     /translation="MAVPKRKTSPSRRGMRRSHEALPREAH"
     gene            317..1699
                     /locus_tag="VFN42_07875"
     CDS             317..1699
                     /locus_tag="VFN42_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET8996570.1"
                     /translation="MTEPVAQVIDAARAFLGERITTNATLREHHSHGQDTQPPVLPDA
                     VAFVETTEEASRILTLCNEHHVPVVAWGAGTSLEGHVTPVRGGITLDLSRMTGILAVS
                     QPDMDCRIEAGVTREQLNTHLRDLGLFFPVDPGTSACTIGGMCATRASGTNAVRYGTI
                     RENVLGLTVAMADGRVIHTGGRVRKSSTGYDLTRLFIGSEGTLGVITEVQMRLHGIPE
                     AMSSATCQFPTLRDAVETVIAIMQCGIPVARMELLDEVQVAACIGYSKLEGLQPLPSL
                     FFEFHGTTAGVAEQAQQAEEIASSFNGRGFRWATDAAERNKLWQARHDALWAALALKP
                     GHKGIATDAIVPISRLDEAILGAKEDIAASGLTAPIVGHVGDGNFHTVILVPPEPDGL
                     ARAWDLDKKIVARALSLGGSCSGEHGVGIGKREFLEQEHGPEALAVMRSVKQALDPRG
                     ILNPGKIFLN"
     gene            1831..2136
                     /locus_tag="VFN42_07880"
     CDS             1831..2136
                     /locus_tag="VFN42_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996571.1"
                     /translation="MHTFRAPQRHRVEGLAFTIPELMLATAWAEYQNMRFSIELDWHV
                     DQVEYEEVIMLGLPGRIGLHCLLWRAADSIVLQPMVGRAQRFPTISQALDAICPEQV"
     gene            complement(2096..2554)
                     /locus_tag="VFN42_07885"
     CDS             complement(2096..2554)
                     /locus_tag="VFN42_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET8996572.1"
                     /translation="MARRTSCGVIVTDGPMLLLGHATGSPRWDIPKGLAEPGEDFRSA
                     ASRELQEETGLEVAPGSLRDLGVHSYMPGKDLALFVWQCAPMPDPRALVCHSGFTLPN
                     GAWRLEFDRFGIFAWDDGLHRVGKNLARVLGQLRPELATPARGRSRPAPG"
     gene            complement(2556..3416)
                     /gene="pdxY"
                     /locus_tag="VFN42_07890"
     CDS             complement(2556..3416)
                     /gene="pdxY"
                     /locus_tag="VFN42_07890"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HET8996573.1"
                     /translation="MNILSIQSWVAYGHVGNASAVFPLQRLGAEVWTINTVQFSNHTG
                     YGDWTGQVFTGEAVRALVDGIAARNVLQQCDAVLSGYMGDPAIGAAILHAVSRVRAEN
                     PAALYCCDPVIGDVAEGVYVRPGIEAFLRDQALPQADIATPNRFELERLTGMPCASLE
                     QARQAAATLAGMLRPDGLRCVLLTSLDSADTPGDHLDLLVHEAGRFHLLRTPLLPVSV
                     NGAGDAIAALFLYHRLRSGNAVTALERAGSSVFGLLRRTAEAGAREILTVAAQDEFVA
                     PSVCFTAQAC"
     gene            3676..3873
                     /locus_tag="VFN42_07895"
     CDS             3676..3873
                     /locus_tag="VFN42_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996574.1"
                     /translation="MRDLRCRWAILDVAESYERMARMAERRLARKQAIAGSSRIALGF
                     ASLVGSGTGWALTYLLHNAVR"
     gene            3959..4429
                     /locus_tag="VFN42_07900"
     CDS             3959..4429
                     /locus_tag="VFN42_07900"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HET8996575.1"
                     /translation="MTWTRRWLIIGCLLSLPVGAMAAREELQVRHAWSRPAMAGRTGV
                     IYLTITDHGAADRLVGAHSAVARKAELHETIDDHGIMKMRAVPSLAIPANGTVTLKPG
                     GYHIMLVGLKHALHAGDSVPLTLTFAKAGKREAMAMVEGPGAGKMRSGGMNMHH"
     gene            complement(4441..5931)
                     /locus_tag="VFN42_07905"
     CDS             complement(4441..5931)
                     /locus_tag="VFN42_07905"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HET8996576.1"
                     /translation="MTAYDRLTTRFARVATISEASAVLGWDSAAMMPPGGAAARGDQL
                     AVLASLAHGLLTARAIADDLAEAEADGPEEDPWRAANLALMRHAHTRATAMPAALVEA
                     QARANSDCEKVWREARRRSDFAQVRPHLSEVLRLVREAARCLAPALGLEPYDALMDGF
                     QRGIGAADVAPVFAQYESFLHTALPQAQERQARGGAANYPRGPFPADQQEALCRRLAE
                     RLGLDFAHARLDRSAHPFSGGTRTDVRITTRYDEADFTQAVLAVVHETGHALYERGLP
                     EAFARQPVGEAAGMAAHESQSLIIEMQACRSDAFLQWLGPELHATFGGAPEPYQPANL
                     ARLWRRVQPSFIRVDADELTYPAHVILRFRLEQALISGDLAVADLPAAWNDGFRALLG
                     ITPPDDARGCLQDIHWYDGAFGYFPSYTLGAMAAAQLMAAARRAVPGMEGALGRGDIS
                     PLLGWLRAKVHGVGSLLGFNDLLRAATGKPLDPSDFQAHLNARYLA"
     gene            complement(5928..6611)
                     /locus_tag="VFN42_07910"
     CDS             complement(5928..6611)
                     /locus_tag="VFN42_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HET8996577.1"
                     /translation="MIRRLFWPGLSTALMFLVLIGLGTWQVQRLHWKRGILAQIARAE
                     AAPPVPLAGDPSPFTKVAATGRLRPDLSAYYGAEVRNTRRGEQMGAYLIEPLERPDAP
                     PLLVELGWVPDVRVRPIAQPEGTTIITGYVHPAEQPNWLSAADNAASRRFYTLDPQAI
                     GAALGLPKVAPFTLVAIGPQPPQLWPDPAKHLPRPPNNHLQYALTWYGLAGVLVVMFV
                     SWSRTRLRE"
     gene            6733..>6868
                     /locus_tag="VFN42_07915"
     CDS             6733..>6868
                     /locus_tag="VFN42_07915"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET8996578.1"
                     /translation="MLPSSLDELARITGRAKSNLSRTLRTMEGYGLVRLEPGERGRIA
                     P"
BASE COUNT         1116 a         2344 c         2416 g          992 t
ORIGIN      
        1 cgtgggcttc acgcggcaag gcctcatggc tgcggcgcat gccgcggcgg gaaggcgacg
       61 tctttctctt cggaacggcc atgggacagt gcccctcaat cctaaatagc tgtggccccg
      121 cgggtgcgag gggcattcac cggatggaaa caacgtgagg ggggccgtct agcagagggg
      181 gccgggggtc gcaaggccac gtccatggtt ccatgccgca gtatgccgtt gggcgccgga
      241 tgggcgtggg gcagggccgc ttgccacgcc gcggccgcgg gtccaatcct cgcgcaaagc
      301 cttgcaagga cccgagatga ccgaaccggt tgcccaggtg atcgacgccg cccgcgcctt
      361 cctcggcgag cggatcacca ccaacgccac gctgcgggag catcactcgc acggccagga
      421 cacccagccg ccggtactgc ccgacgcggt cgccttcgtg gaaaccaccg aggaagccag
      481 ccgcatcctg acgctgtgca acgagcacca cgtgccggtg gtggcctggg gcgccggcac
      541 ctcgctggag ggccacgtca ccccggtgcg cggcggcatc acgctcgacc tgtcccgcat
      601 gaccgggatc ctcgccgtca gccagcccga catggactgc cgtatcgagg ccggcgtaac
      661 gcgtgagcag ctcaacaccc acctgcgcga cctcggactg ttcttcccgg tcgatcccgg
      721 cacctcggcc tgcaccatcg gcggcatgtg cgccacccgc gcctccggca ccaatgctgt
      781 ccgctatggt accatccggg agaacgtact cggcctgacc gtggcgatgg ccgacgggcg
      841 ggtgatccac accggggggc gggtccgcaa atcctccacc ggctacgacc tgacgcgtct
      901 gttcatcggc tcggaaggca cgctcggcgt catcaccgag gtgcagatgc gcctgcatgg
      961 catcccggag gcgatgtcgt ccgccacctg ccagttcccc accctgcgcg acgcggtgga
     1021 gacggtgatc gccattatgc agtgcggcat tccggtcgcc cgcatggagc tgctggacga
     1081 ggtgcaggtg gcggcctgca tcggctattc caagttggag gggctgcaac cgctgcccag
     1141 cctgttcttc gagttccacg gcaccacggc cggcgtggca gagcaggcac agcaggcgga
     1201 ggagatcgcc agcagcttca acggccgcgg tttccgttgg gcgaccgacg cggcggagcg
     1261 caacaagctg tggcaggcgc gccatgacgc gctgtgggcg gcgctggcgc tgaaaccggg
     1321 ccacaagggc atcgccactg atgcgatcgt accgatctcc cgcctggacg aagccattct
     1381 cggcgcgaaa gaggacatcg ccgcgtcggg cctgaccgcg ccgatcgtcg gccatgtcgg
     1441 cgacggcaat ttccacacgg tgatcctggt gccgccggag ccggacgggc tggcacgcgc
     1501 ctgggacctg gacaagaaaa tcgtcgcccg cgcgctgtcg ctgggtggct cgtgcagcgg
     1561 cgagcacggt gtgggcatcg gtaagcggga attcctggaa caagagcacg ggccggaggc
     1621 gctggcggta atgcgctcgg tgaagcaggc gctggacccg cgcggcattc tcaatccggg
     1681 caagatcttc ctgaactgaa gccaagtgac ggcggaatcc cgtgcctgca tacctgagtg
     1741 ttttgcacgt acgggaacat ccgaattttc tccgtcctgc cggtgtgctc ccattccgct
     1801 gccgattggc acagcccggg agatcccccc atgcacacat tccgcgcacc acagaggcac
     1861 cgggtcgaag gtctagcctt caccattccc gagctgatgc tggcaacggc ctgggcggaa
     1921 taccagaaca tgcggttcag catcgaactc gactggcatg tcgatcaggt cgagtacgag
     1981 gaggtcatca tgctgggact gcccggccgc atcggcctgc attgcctgtt gtggcgtgcg
     2041 gcggacagca tcgtgctgca gccgatggtg gggcgggcgc agcgcttccc gacgatcagc
     2101 caggcgctgg acgcgatctg ccccgagcag gtgtagccaa ctccgggcga agctggccaa
     2161 gcacgcgcgc gaggttcttg ccgacacggt gcaacccgtc gtcccaggcg aagatgccga
     2221 agcgatcgaa ctccaaccgc caggccccgt tcggcagcgt gaacccggaa tggcagacca
     2281 gggcccgcgg atccggcatt ggcgcgcatt gccagacaaa cagcgccagg tccttgccgg
     2341 gcatgtagct gtgcacgccg agatcgcgca gcgacccggg cgccacctcc aggccggtct
     2401 cctcctgcaa ttcccgcgat gcggcggacc ggaagtcttc accgggttcg gccagtccct
     2461 tcggtatgtc ccagcgcggc gagccggtgg cgtggcccag cagcagcatg gggccatcgg
     2521 tgacgatcac gccgcaggag gtgcggcgcg ccacgtcagc aggcctgggc cgtgaagcaa
     2581 accgacggtg cgacaaactc gtcctgcgcc gccacggtca ggatctcacg cgcaccggcc
     2641 tccgcggtcc ggcgcagcag cccgaacacc gaggagccgg cccgttccag cgcggtcacc
     2701 gcattgccgc ttcgcaggcg gtggtagagg aacagcgcgg caatcgcgtc gccggcgccg
     2761 ttcacgctga ccggcagcag cggcgtgcgc agcagatgaa aacgcccggc ctcatgcacc
     2821 agcaggtcca ggtggtcgcc cggcgtgtcc gcactgtcca ggctggtcag cagcacgcaa
     2881 cgcagcccgt ccggccgcag catgccggcc agtgtcgccg ccgcctgcct ggcctgttcc
     2941 agcgacgcgc agggcattcc ggtcagccgt tccagctcga accggttggg cgtggcgatg
     3001 tcggcctgcg gcagtgcctg gtcgcgcagg aacgcctcga tgccggggcg cacatagacg
     3061 ccttccgcca cgtcgccgat caccggatcg cagcaataca gcgcggccgg gttctctgcc
     3121 cggacgcggc tcaccgcgtg caggatcgcc gcgccgatcg ccgggtcgcc catgtagccg
     3181 gacagcaccg cgtcgcactg ctgcagcaca ttgcgtgcgg cgatgccgtc caccagcgcg
     3241 cgaaccgcct cgccggtgaa cacctggccg gtccaatcgc cgtatccggt gtggttggag
     3301 aactggacgg tattgatcgt ccacacctcc gcgccgagcc gctgcagcgg aaacaccgcg
     3361 ctggcattgc cgacatggcc gtaggcgacc caggactgga tggacaggat attcatgcca
     3421 aagacatccg atagagcgag atctgtctca ccgcaaaaac tcctcaatca cactggggtc
     3481 tcaagcaagc gaatacggca tgtgacgacg ccgtgagaca gccatgtcgg aacagcatgt
     3541 atccatgata tcggcatgag gtattccggc gctcgaccaa acaggatgga gactgacagc
     3601 ggtatgattc cggttgcgga cgacgtcgct ctctggcgga agcgggcggc agccacgcgg
     3661 gatgctgcac ggcagatgcg cgatctccgg tgccgctggg ccatcctgga cgtcgccgag
     3721 agctacgagc gcatggcgcg gatggccgag cgccggctgg cccgcaagca ggcgatcgcg
     3781 ggcagttccc ggatcgctct tggcttcgcc agcctggtcg gtagcggcac cggctgggca
     3841 ctgacctacc tgctgcacaa cgcggtccgc taggcgcgtc tcccgcggcg gccgggagct
     3901 tgcccggacg gccgacaccc ggcaacgtcg gcggcaaccg gaaagaggac caccgacaat
     3961 gacttggacg cgccgctggc tgatcatcgg ctgcctgctg tctctgcccg tcggggccat
     4021 ggcggcgcgg gaggagttgc aggtgcggca tgcctggtcg cgtcccgcca tggccgggcg
     4081 gaccggggtc atctacctga ccatcaccga tcatggcgcc gcggaccggc tggtcggcgc
     4141 gcattcggcg gtggcccgga aagccgaact gcacgaaacc atcgacgatc acggcatcat
     4201 gaagatgcgc gcggtcccct cgctggccat tccggcgaac ggcaccgtga ccctgaagcc
     4261 cggcggctac cacatcatgc tggtgggcct gaagcacgcg ctgcatgcag gcgacagcgt
     4321 gccgctgacc ctgaccttcg ccaaggccgg caaacgggag gcgatggcga tggtggaggg
     4381 gcccggcgcg gggaagatgc ggtccggcgg gatgaacatg catcactagc cgcgcccggt
     4441 tcaggccagg tagcgcgcgt tcaggtgcgc ctggaagtcc gacgggtcca gcggcttgcc
     4501 ggtggcggcg cgcagcaggt cgttgaagcc gagcaggctg ccgacgccgt gcaccttcgc
     4561 gcgcaaccag ccgagcaggg gggagatgtc gcctcggccc agcgccccct ccatgccggg
     4621 caccgcccgc cgcgccgcgg ccatcagttg ggccgccgcc atcgccccca gtgtgtagct
     4681 ggggaaatag ccgaacgcgc cgtcgtacca atggatatcc tgcaggcagc cgcgcgcgtc
     4741 gtccggcggc gtgatgccca gcagcgcgcg gaacccgtcg ttccaggcag cgggcaggtc
     4801 cgccaccgcc agatcgccgg aaatcagcgc ctgctccagc cggaagcgca ggatcacgtg
     4861 cgccgggtag gtcagttcgt cggcatccac ccggatgaag ctgggctgca cgcgccgcca
     4921 tagccgcgcg aggttggccg gctggtaggg ttcgggcgca ccgccgaacg tcgcgtgcag
     4981 ctccgggccg agccattgca ggaaggcgtc ggagcggcag gcctgcatct cgatgatcag
     5041 cgactggctt tcatgcgcgg ccatgccggc ggcctccccc accggctggc gggcgaatgc
     5101 ttccggcagg ccgcgctcat acagggcgtg gccggtttcg tgcaccacgg ccagcacggc
     5161 ctgggtgaag tcggcctcgt catagcgcgt ggtgatccgc acatcggtgc gggtgccgcc
     5221 ggagaacgga tgcgccgagc ggtccagccg cgcgtgcgcg aagtccaggc cgagccgctc
     5281 ggccagccga cggcacagcg cttcctgctg gtccgcggga aacgggccac ggggataatt
     5341 cgccgcgccg ccgcgggcct ggcgttcctg cgcctggggc agggcggtat gcaggaagct
     5401 ctcgtactgc gcgaagaccg gcgccacatc cgccgcgccg atgccgcgct ggaacccgtc
     5461 catcagcgcg tcatagggct ccagtccaag cgctggtgcg aggcagcggg cggcttcccg
     5521 aaccaggcgc agcacctctg acaggtgcgg gcgcacctgc gcgaagtcgc tgcggcggcg
     5581 tgcctcgcgc cacaccttct cgcagtcgga attggcgcgg gcctgcgcct ccaccaaggc
     5641 ggcgggcatc gcggtggcgc gggtgtgggc gtggcgcatc agtgccaggt tggcggcgcg
     5701 ccagggatcc tcctccggtc catcggcttc cgcctccgcc agatcgtcgg cgatcgcccg
     5761 ggcggtcagc aggccatgcg cgaggctggc gagcaccgcc aactgatcgc cgcgcgccgc
     5821 cgcgccgccg ggcggcatca tcgccgcgga gtcccagccc agcacggcgg aggcctcgct
     5881 gatcgtcgcg acgcgggcga agcgcgtggt cagccggtcg taggcggtca ttcgcgcaac
     5941 ctcgtgcggc tccaggacac gaacatcacc acgagcaccc cggcgaggcc ataccaggtc
     6001 agggcgtatt gcagatgatt gttcggcgga cgcggcaggt gtttggccgg atcgggccat
     6061 agctgcggcg gctgcggtcc gatggcgacc agggtgaagg gcgcgacctt gggcaggccg
     6121 agcgcggcgc cgatcgcctg cgggtccagc gtgtagaacc ggcggctggc ggcgttatcc
     6181 gcggcgctca gccagttggg ctgctcggcc gggtgcacgt agccggtgat gatcgtcgtg
     6241 ccctcgggct gtgcgatcgg ccgcacgcgc acatccggca cccagccaag ctccaccagc
     6301 agagggggcg cgtcgggccg ttccagcggc tcgatcagat aggcgcccat ctgctcgccg
     6361 cggcgggtgt tgcggacttc ggcgccgtaa taggcggaca ggtcggggcg cagccggccg
     6421 gtcgccgcaa ccttggtgaa gggcgaggga tcaccggcca gcggcaccgg cggcgcggcc
     6481 tcggcccggg cgatttgcgc caggataccg cgcttccagt gcaggcgctg gacctgccac
     6541 gtgccgaggc cgatcagcac gaggaacatc agcgcggtgg acaggccggg ccagaacagg
     6601 cggcggatca aggcggcagc gacctcaaag aacgtgttgg atggcgcggg catagcccgg
     6661 cggggtgtcg gatgccagcg ccggggcgac gcagcgctgt cacggcgcga tccttgttct
     6721 cgcgagcacc gcatgttgcc cagctcgctg gatgagctgg cgcggatcac cggcagggcc
     6781 aagtcgaacc tttcgcgcac gctgcggacg atggaaggtt atggcctggt tcgcctggaa
     6841 cccggcgagc gcgggcgcat cgcgccca
//
LOCUS       DASSZH010000363         3968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_8693, whole genome shotgun sequence.
ACCESSION   DASSZH010000363 DASSZH010000000
VERSION     DASSZH010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8693"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..1035
                     /locus_tag="VFN42_07920"
     CDS             202..1035
                     /locus_tag="VFN42_07920"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET8996579.1"
                     /translation="MNALHAMVIALVQGATELFPVSSLGHAVLLPALLHWPIDEHARD
                     FLPFLVMLHVGTALALLGYFWRDWWALGTGILGAGTPHQIRESRRVLLLIIIATIPAV
                     IVGFLLEHFFRNLFGTPLVAAAFLMVNGALLLVGEQLRGGGNRPLSSLTALDALIIGC
                     WQCSALIPGISRSGATIVGGLLRGIDHEGSAHFSFLIATPIILGATVLEVPKLLHTGV
                     PHAVFAISVAAAVVAGLTALASTAFLMHYFREHDRWALNPFAYYCILAGMVSAGVLLA
                     F"
     gene            complement(1047..2054)
                     /locus_tag="VFN42_07925"
     CDS             complement(1047..2054)
                     /locus_tag="VFN42_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HET8996580.1"
                     /translation="MLSLEHASRFAGIALGHVTREYPNKLDQVLTGPADLLPPRALHP
                     IFYGSFDWHSCVHAYWLLARLLRRHPEMPQAAGIRAQFRSALTADNVAAECAYLARPL
                     TATFERPYGWAWLLMFAAELNRQDTAEARHWSATLAPLAEAFSHRFLQWLPKAAYPSR
                     SGLHSNTAFALILALDYSAAAGDAALAEAGRGAARAWYLQDADCQAWEPSSEDFLSPC
                     LAEALCMQRVLPPEEFLPWFRRFLPQILRHQPATLFQPVSVSDRSDGRIAHLDGLNLS
                     RAWCWSELARALPVDAPERPVMGDAASRHLQASLPHVSGDYMGEHWLASFATLALEAA
                     D"
     gene            complement(2073..2570)
                     /locus_tag="VFN42_07930"
     CDS             complement(2073..2570)
                     /locus_tag="VFN42_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HET8996581.1"
                     /translation="MPATQDELFALFDRLGIAHSTVTHPPFFTVEEGRPWHDKIPGLH
                     CKNLFIKDRKGGIWLVVMPANKRANLGRLDKTLQAPRFSFARPELLQEVLELTPGSVT
                     PFGLINDHQRRVTVILDQDMLDAEQVNFHPLHNAASTTLRSADLLRFIRALGYEPIIV
                     RLPEP"
     gene            2709..3389
                     /locus_tag="VFN42_07935"
     CDS             2709..3389
                     /locus_tag="VFN42_07935"
                     /inference="COORDINATES: protein motif:HMM:NF018917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HET8996582.1"
                     /translation="MSPLLSPLLRALDQLGDRVFLGVLLRSAAWSALCFVLLYGATFW
                     GVRHMLHLHGMLAWAAGLLGAVGATLLALWLFLPVAAVIATLYIERIARAVERRYYPW
                     LPPPRGAPIGEQIADGLGLGVRILLLNVIALVLVVLLPGIGLVLGWAIAAFGIGRGLF
                     VAVAMRRMPRPAAMALYHRFRSAVLVQGGLMALAAYVPVLNLLIPVLGTAAMVHVLDH
                     ALTGSGEA"
     gene            3726..>3968
                     /locus_tag="VFN42_07940"
     CDS             3726..>3968
                     /locus_tag="VFN42_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019085931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET8996583.1"
                     /translation="MSRPPFPATPGTQPATPGAAAARPWIAPARPAARDPGERRTLVV
                     GRGISVQGTVQDAERLVVEGTVEATMIHATELSIAPG"
BASE COUNT          571 a         1317 c         1383 g          697 t
ORIGIN      
        1 gatgcctgga aggcgcagtt ctttcattat ggagaggcgt tccgcattgg cgcggccaac
       61 cgggcgcgcg gccaccgccc ggccgggctg cgcacgctgt ggcgttactg gcgggcacaa
      121 tcgcgtttga tggcgcggta cccggcgtac gattgagttt tcgcggttgc ggaatccgca
      181 ggcttcagcc acgaagcgcg catgaatgcc ctccatgcga tggtcatcgc cctcgtgcag
      241 ggtgcgaccg aactgttccc ggtcagcagc ctgggccatg ccgtgttact gccggcgctg
      301 ttgcactggc ccatcgacga gcacgcacgg gacttcctcc cgttcctggt catgctgcat
      361 gtcggcacgg cgctggcgct gctcggttat ttctggcgcg actggtgggc gctgggcacc
      421 ggcattctag gcgccggaac cccccaccag atccgggaaa gccggcgggt cctgctgctg
      481 atcatcatcg ccaccattcc ggcggtcatc gtgggcttcc tgctggagca tttcttccgc
      541 aatctgttcg gcaccccgct ggtcgccgcc gccttcctga tggtgaatgg cgccctgctg
      601 ctggtcggcg agcagttgcg cggcggcggc aaccggccgc tgtccagcct gaccgcgctg
      661 gacgcattga tcatcggttg ctggcaatgc agtgcgctga ttccgggcat ttcccgttcc
      721 ggcgcgacca tcgtgggcgg tctgctgcgc ggcatcgacc atgagggatc ggcacatttc
      781 tccttcctga tcgccacgcc gatcatcctg ggagcaacgg tgctggaggt gccgaagctg
      841 ctgcataccg gggtgccgca tgcggtgttc gcgatttcgg tggcagccgc ggtggtcgcc
      901 gggctgaccg cgctggccag cacggccttc ctgatgcact acttccgcga acacgaccgc
      961 tgggcgctga atccgttcgc ctattactgc atcctggccg gcatggtgag cgcgggcgtg
     1021 ctgctggcgt tctgaggcgc cggcggtcaa tccgccgcct caagcgccag ggtggcgaag
     1081 ctcgccagcc agtgctcgcc catgtagtcg cccgacacat gcggcaggct ggcttgcagg
     1141 tgccggctgg cggcgtcccc catcaccggc cgttccggcg catccactgg caaggcccgg
     1201 gccagttcac tccagcacca ggcacggctc agattcagcc catccagatg cgcgatccgg
     1261 ccatcgctgc gatcgctgac gctgaccggc tggaacagcg ttgccggctg gtggcgtagg
     1321 atttgtggca gaaagcggcg gaaccacgga aggaactcct ccggcggcag cacgcgctgc
     1381 atgcacagcg cctccgccag gcatggtgac aggaaatcct cgctgctcgg ctcccaggcc
     1441 tggcaatcgg catcctggag gtaccaggcc cgggcggcgc ccctgcccgc ctcggccagc
     1501 gccgcatcgc cggccgcggc ggaataatcc agcgccagga tcagggcgaa ggcggtgttg
     1561 ctgtgcaggc cgctgcggct tgggtaggcg gccttgggca gccactgcag gaagcgatgg
     1621 gagaacgctt cggccagcgg cgccagggtg gcagaccagt gccgcgcctc tgccgtgtcc
     1681 tgccggttca gctcagcggc gaacatcagc agccacgccc agccgtatgg ccgctcgaac
     1741 gtggcggtca gcggccgagc gagataggcg cattcggccg cgacattgtc cgcggtcagg
     1801 gcggagcgga actgcgcgcg aatgcccgcc gcctgcggca tttccgggtg ccggcgcagc
     1861 agccgggcca gcagccagta ggcgtgcacg caggaatgcc agtcgaaact gccgtagaag
     1921 atcggatgca gggcgcgcgg cgggagcaga tccgccgggc cggtcagcac ctggtccagc
     1981 ttgttgggat attcccgggt gacatggcca agggcgatcc cggcaaagcg cgacgcgtgt
     2041 tccaggctga gcatgcattc ctccggggga gttcagggct ccggcagccg cacgatgatg
     2101 ggctcgtagc ccagggcacg gatgaaacgc agcaggtcgg cggagcgcag tgtggtggac
     2161 gcggcattgt gcagcggatg gaagttcacc tgctctgcgt ccagcatgtc ctggtccagg
     2221 atcacggtga cccggcgctg gtggtcgttg atcaggccga atggggtgac cgagccgggg
     2281 gtcaattcca gcacctcctg cagcagttcc ggccgagcga aggagaagcg cggagcctgc
     2341 agggtcttgt cgagccggcc gagattggcc cgcttgttgg ccggcatcac caccagccag
     2401 atgccgccct tgcggtcctt gatgaacagg ttcttgcagt gcaggcctgg gatcttgtcg
     2461 tgccagggac gcccctcctc gaccgtgaag aacggcgggt gcgtcacggt gctatgggcg
     2521 atgccgagac gatcgaacag ggcgaagagt tcgtcttggg tggcgggcac gctgctatgg
     2581 gagagttgag gggaccttgc ttgccggtgt gcggcaagca gcccgccaga tcaagccggg
     2641 cgccacaaga ccgggccgcg gcagcagggt ccttgcacgg cgaacacacc tgcggcaccc
     2701 accggcgcat gtccccgctt ctctccccgc tgctgcgtgc gctggaccaa ttgggcgatc
     2761 gcgtgttcct cggcgtgctg ctccgctccg ccgcctggtc cgccctgtgt ttcgtccttc
     2821 tgtacggggc gacgttctgg ggcgtccggc acatgctgca tctgcacggc atgcttgcct
     2881 gggccgccgg cctgctcggc gcggtgggcg cgacgctgct ggccttgtgg ctgttcctgc
     2941 cggtcgcggc ggtgatcgcc acgctgtaca tcgaacgcat cgcccgcgct gtggagcgac
     3001 gctactatcc ctggctgccg ccgccgcgcg gggcgccgat cggcgagcag atcgccgatg
     3061 gcctgggtct tggcgtccgc atcctgctgc tgaacgtcat cgccctggtc ctggtggtgc
     3121 tgctgccggg catcggcctg gtgctcggct gggcgatcgc ggccttcggt atcgggcgcg
     3181 gcctgttcgt cgccgtggcg atgcgccgca tgccgcggcc ggccgcgatg gcgctgtacc
     3241 accgcttccg ctctgccgtg ctggtacagg gaggtctcat ggcgctggcc gcctatgtcc
     3301 cggtgctgaa cctgctgata ccggtgctcg gcacggccgc catggtgcat gtgctggacc
     3361 acgcattgac cgggtcaggc gaagcgtgac taatactgaa gcggatcaat gtggataacc
     3421 gtacgtaagg cgttgtttcg ccagcatccg gtatgttagg ggcggacgac ttcagagggt
     3481 ccgctatgtc atccagttcc acggggattc ccttgttcaa acgccgcaag cctgacgaaa
     3541 tgccggccgg atcgggggga actctgccgg ctggcctgtc cgggaccgac acttccggcg
     3601 gattcgccga gaatgccgac cgcactgaac tgcatccgcg caaccctggc gtagcttccg
     3661 gcccggttga ttccgggctg ggcgtgccgc cgttccgacc ggcccagccc aaggaggctc
     3721 aatccatgtc ccgtccgcct ttccccgcca cgcccggaac ccagcccgcg actccgggcg
     3781 ccgccgccgc gcgtccctgg attgcgccgg cacggccggc cgcgcgcgac ccgggcgagc
     3841 gccgcaccct ggtggtgggc cgtggcatca gcgtgcaggg cacggtgcag gacgccgaac
     3901 ggctggtggt cgaaggcacg gtcgaggcca ccatgatcca tgccaccgag ctgtcgatcg
     3961 cgccgggc
//
LOCUS       DASSZH010000364         3269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1372432, whole genome shotgun sequence.
ACCESSION   DASSZH010000364 DASSZH010000000
VERSION     DASSZH010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372432"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VFN42_07945"
     CDS             complement(<1..481)
                     /locus_tag="VFN42_07945"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET8996584.1"
                     /translation="MKAAEAVVEILKREGVQTLFCFPTTSLIETAVAGGLRPVICRQE
                     RVGVDMADGYARVTNGKPPGVFAMQYGPGAENAFPGIATTYSDSVPVLFLPLGHARDN
                     AQVYPLFSSARTYASVTKQVETLAVPEQAPAAMRRAFSALKNGRLGPVMVELPRDLLD
                     "
     gene            complement(627..1628)
                     /locus_tag="VFN42_07950"
     CDS             complement(627..1628)
                     /locus_tag="VFN42_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HET8996585.1"
                     /translation="MRANIPDTQTVIEIKQPGGPEVLVPARRPVPQPKPGEVLIRIAA
                     GGVNRADCLQRMGKYPMPAGASDIPGLECSGTVVQRGEGVDQWQEGDQVCALLVADGY
                     GEYAAVPAVQCLPVPQGVSLVDAGGLAETFCTVWTNVFERMHLQPGESFMVQGGTSGI
                     GITAIQLAKAWGCKVLATAGSDEKCAACRDFGADVAINYRTQDFVQVGKEATDGRGVD
                     VILDMVGGDYIPRQVELLAREGRLGFVALMGGTKATVDFGAVHRKHLTITGSTLRGRS
                     IAAKASIVSALREKVWPMWAQGKLRTATYRTFPLEQASEAHRLMESSQHIGKILLVP"
     gene            1767..2582
                     /locus_tag="VFN42_07955"
     CDS             1767..2582
                     /locus_tag="VFN42_07955"
                     /inference="COORDINATES: protein motif:HMM:NF014635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HET8996586.1"
                     /translation="MNDAPTLSDAPALSGKDIAELAEQLNQLLKLRTFPIGMKLFEDV
                     EQMAAVPGLRRPPKGKTFSTCQLVTQARMAGFTLGITTENVPPFSSCSSVIGLDAPGE
                     IYTSGRKMEGVWFENRDAAAAHQAGMSRVPPGRYHGLAISPLRTSRLDPPDICLFYGN
                     PAQMILFINGLQWKNYRRYDFSITGESACADSWGHALKTREVSLSIPCYAERRYGGVA
                     DDEMLMACAPADLQRAVTGLQGLAKAGLRYPIMPFGPQAEPAEGMAKSYAGKG"
     gene            2742..>3269
                     /locus_tag="VFN42_07960"
     CDS             2742..>3269
                     /locus_tag="VFN42_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HET8996587.1"
                     /translation="MPSPGSELRTGVQGLDEILCGGYQSSRVYLIEGEPGAGKTTLAF
                     QFLREGCARGERGLYIALSESRDELLHTAASHGWSLDGIDIFELVPPEFSLDPQHEQS
                     VVYTADLELGETVRIVMAEVERLRPKRIVFDSLAEIRLLAQTSLRYRRQVLALKYLFA
                     KLGATGLLLDDMARPE"
BASE COUNT          526 a         1124 c         1066 g          553 t
ORIGIN      
        1 ggtccaacag gtcgcgcggc agctccacca tcaccgggcc cagccgtccg ttcttcagcg
       61 cactgaaggc gcggcgcatg gcggcgggcg cctgctccgg cacggccaga gtctccacct
      121 gcttggtgac ggaggcatag gtgcgggcag agctgaacag cggatagacc tgcgcattgt
      181 cgcgcgcatg gccgaggggc aggaacagca ccggcaccga atcggaatag gtggtggcga
      241 tgccggggaa ggcgttctcc gcgccgggtc cgtactgcat ggcgaacacg ccgggcggct
      301 tgccgttggt gacgcgggca tagccgtcgg ccatgtccac gccgacacgc tcctgccggc
      361 agatcaccgg tcgcaggccg ccggccaccg cggtttcgat cagcgatgtg gtggggaagc
      421 agaacagtgt ctgcacgcct tcccgcttga ggatttcgac gaccgcttca gctgctttca
      481 tttggagttt tctccttctg cccctgacgt cctggccggg cttgacccgg ccatcttccc
      541 cttgccgacc gggtcggtca gcgccggcgg cggcccgatc gccggtgtgt ggatggccgg
      601 gtcaagcccg gccatgacgc aacaagtcac ggtaccagca ggatcttgcc gatgtgctgg
      661 ctggattcca tcagccgatg agcctccgac gcctgttcca gcgggaacgt cctgtaggtc
      721 gcggtgcgca gcttgccctg cgcccacatc ggccagacct tctcccgcag cgccgagacg
      781 atcgaggcct tcgccgcgat gctgcgcccg cgcagggtgg agccggtgat cgtcaggtgc
      841 ttgcgatgca ccgcgccgaa atccacggtt gccttggtgc cgcccatcag cgcgacgaag
      901 cccagccggc cctcgcgcgc gagcagttcc acctggcgcg ggatgtagtc gccgccgacc
      961 atgtccagga tcacgtcaac gccgcggccg tcagtcgcct ccttgcccac ctgcacgaaa
     1021 tcctgcgtgc ggtagttgat cgccacgtcg gcgccgaagt cgcggcacgc ggcacatttc
     1081 tcgtcggaac ccgcggtggc cagcaccttg cagccccagg ccttcgccaa ttggatcgcg
     1141 gtgatgccga tgccgctggt gccgccctgc accatgaagc tctcgccggg ctgcaggtgc
     1201 atgcgctcga acacgttcgt ccacaccgtg cagaacgtct ccgccagccc gccggcatcc
     1261 accaggctga cgccttgcgg caccggcagg cactgcactg ccggcaccgc ggcgtattcg
     1321 ccgtagccat ccgccaccag cagcgcacac acctggtcgc cttcctgcca ctggtccacg
     1381 ccctcgcccc gctgcaccac ggtgccggag cattccagcc cgggtatgtc ggaggcgccg
     1441 gccggcatcg ggtatttgcc catgcgctgc aggcagtccg cgcggttcac cccgccggcg
     1501 gcgatgcgga tcagcacttc gcccggcttc ggctgcggta ccggccggcg tgccggaacc
     1561 agcacctccg gccctccggg ctgcttgatc tcgatgacgg tctgggtgtc ggggatattg
     1621 gcgcgcactg gtggtcctcc ggctgacggc ggcggcaccc tgccatagcg gcagccggcg
     1681 tgcacgcccc ctccggcttt cccttaacgg cccggcggtg gcagcatgcc acgcagcgcc
     1741 gccccaaggc ggcagggaga aacgcgatga acgacgcacc cacgctgagc gacgcaccgg
     1801 ccctgtccgg caaggacatt gccgaattgg cggagcagct caaccagttg ctgaaactgc
     1861 gcacgtttcc gatcggcatg aagctgttcg aggatgtgga gcagatggcc gcggtgcccg
     1921 gactgcgccg gccgcccaag ggcaagacgt tctccacctg ccagctcgtc acccaggcgc
     1981 ggatggccgg cttcaccctg ggcatcacca ccgagaacgt gccgcccttc tccagttgct
     2041 ccagcgtcat cggcctggat gcgccgggag agatctatac ctccggccgc aagatggagg
     2101 gtgtctggtt cgagaaccgg gacgcagcag cggcgcacca ggccggcatg tcgcgcgtgc
     2161 cgccggggcg ctaccatggc ctggcgatct cgccgttgcg caccagccgg ctggacccgc
     2221 cggacatctg tctgttctac ggcaaccccg cgcagatgat cctgttcatc aatggcctgc
     2281 agtggaagaa ctatcgccgc tacgatttca gcatcaccgg cgagagcgcg tgcgccgatt
     2341 cctggggcca tgcgctgaag acacgcgagg tgagtctctc catcccctgc tacgccgagc
     2401 gccgctacgg cggcgtggcg gacgacgaga tgctgatggc ctgtgcgccg gcggatctgc
     2461 agcgggcggt caccggcctg caggggctgg ccaaagcggg gctgcgctac ccgatcatgc
     2521 cgttcggtcc acaggctgaa ccggcggaag gcatggcgaa gagctacgcc gggaaaggct
     2581 gacccacccc gggtgttcac agaccgcgcg ttgccgcgat tcgatcaact gttattgctt
     2641 tctcatcggc accgagcttg cctacgtgtc tgggcaccca ttgtgagttc gcaacctaac
     2701 tagacttatt cggttatcgg atggacgccc tacccagacc aatgccctcg cccggctccg
     2761 aactgcgaac cggggttcag gggctcgatg aaatcctgtg cggtggctat caaagcagcc
     2821 gggtttacct gatcgaaggc gaacccggcg ccggcaagac caccttggct ttccagttcc
     2881 tgcgcgaagg ctgtgcccgc ggcgagcgcg gcctgtacat cgcgctgtcg gaaagccggg
     2941 acgaattgct gcacacagcg gcgtcgcacg gctggtcgct cgatggtatc gacatcttcg
     3001 agctggtgcc tcccgagttc agcctggacc cgcaacacga gcagagcgtc gtctacacgg
     3061 ccgacctgga actcggcgag acggtgcgca tcgtcatggc ggaggtggag cgcctgcgcc
     3121 cgaaacgcat cgtattcgac agcctggcag aaattcgtct gctggcgcag acttcgctgc
     3181 gatatcgccg gcaggttctg gcgttgaaat atctgttcgc gaaactgggc gcgaccggct
     3241 tgctgctgga tgacatggcg cgcccggag
//
LOCUS       DASSZH010000365         8099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1287090, whole genome shotgun sequence.
ACCESSION   DASSZH010000365 DASSZH010000000
VERSION     DASSZH010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8099
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287090"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            379..1902
                     /gene="xseA"
                     /locus_tag="VFN42_07965"
     CDS             379..1902
                     /gene="xseA"
                     /locus_tag="VFN42_07965"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003617195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET8996588.1"
                     /translation="MDDLPAAPISNIPEFTVSEISGAVKRTLEGTFGRIRVRGEITEL
                     KRYPSGHIYFSLKDENAKLAGVVWKSAVPRLGMAPENGVEVIATGRISAYGERSSYQL
                     IVERMEYAGAGALLARIEMLRVRLAAEGLFDTDRKRKLPVLPAVIGVVTSERGAVIQD
                     IRTTLARRFPRTLLLWPVPVQGEGSAERIAAAIAGFDALSPGGAVPRPDVLIVARGGG
                     SLEDLMAFNEEIVVRAAAACRIPLISAVGHETDTTLIDFASDRRAPTPTAAAELAVPA
                     RADLIADLAQREARLVGALNRLTQERRLRLSRAERGLPDLPTLLGTARQRLDDRAERL
                     RLALPNLVERRRAALVAAERRLPDLPAMLHARRERVHERGVRLVLALPNLTAARRSSL
                     ELTSHQLIACLRHRVAAIHNSANRTLARLTGAPLRSTLREARARLDGAGGRLEAMSPL
                     AVLQRGYVVVSDSAGHTLTNAEAVKPGARLRLRFCDGELGATADGGRKSDRQGQLPL"
     gene            complement(1930..2505)
                     /locus_tag="VFN42_07970"
     CDS             complement(1930..2505)
                     /locus_tag="VFN42_07970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HET8996589.1"
                     /translation="MIFCSAWILTESLPARASIPPANSLQAQQEARRLDELPPLVPHG
                     KASIDRSGRKQKGKASWYGPGFAHKLMANGDRMNPHSNIAASKTLPLGTTAKVVNLKN
                     GKSATVKIEDRGPFVDGRVVDVSPKVANELGLKKVGVAPVVVKPIAVPLPDGKVKLGA
                     GAAGLPPKQVEQATRTTKALAGGGSTETAER"
     gene            complement(2780..3911)
                     /gene="prfB"
                     /locus_tag="VFN42_07975"
     CDS             complement(join(2780..3841,3843..3911))
                     /gene="prfB"
                     /locus_tag="VFN42_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004873.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET8996590.1"
                     /translation="MSAESDALNEQIKQSVALLRRHLDWDVAEGRLAELNHRAEDPNL
                     WNDPAAAQKLMRERNLLATQIDGVRKLEADVSDTLELIALAEADSDEALVADGMESLR
                     ALAEEAKRREIESLLAGEADANDAYVEINAGTGGTEAQDWAEMLMRMYLRWAEQHGYK
                     VQVMEESEGEQAGIKSCTLLVSGPNAYGWLKTEAGVHRLVRISPFDSAARRHTSFASV
                     WVYPVVDDNIDIEIDPSDLKVDTYRASGAGGQHVNKTESAIRITHMPTGIIVACQTDR
                     SQHRNRATAMAMLKARLYEAELQKREAAAAAVESAKTDIGWGHQIRNYVLAPYQLVKD
                     LRTGVEKGNPDAVLDGDLDPFMAAALAARAGATRSEASAQAR"
     gene            complement(3942..6440)
                     /locus_tag="VFN42_07980"
     CDS             complement(3942..6440)
                     /locus_tag="VFN42_07980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HET8996591.1"
                     /translation="MRGLLVAFLGLMLLGLVVGSGGAYFLWLHFSANLPSVDGLKNYQ
                     PKEMSRVFAGNDRLLADLAAERRIFMPINAIPKRVQEAFVSAEDQNFWVHGGIDPAAI
                     VRAGLTDLMRIGTGRRPLGASTITQQVVKNMLLNDRVSLSRKVREALLAIRMQQVMSK
                     QRILELYLNEIYLGMGSYGVAAAAEAYFNKPLDQLDLAQDAFLAALPKAPNNYNPWKY
                     PEAAKARRDWVLDRMADDHNITVAQAAAAKAQPILPTEFHHPAPIPGAEWFTEEVRRE
                     LVAKFGNDVTTEGGLMVHTSLDPKLQNAAEKAVRDGLMVYDHRRGGWRGPVTHLAVGS
                     AQDWPAALAKVSAPAGMLGDWRLAVVLSTSNTEAKVGWLSPESQPNAQPQPRTGVLPL
                     SDVAWARAAHDDRLGPRPRRITDVVQDGDVIMIQADPAEPARQAGPMKVALRQIPKVQ
                     GALVSMDPTTGRVLAMVGGWSYDMSQFNRATQAQRQPGSSFKPFVYLAAMEKGLSPSQ
                     RFLNAPVVLDTPEGKWRPGNYEGTFGGPTSLRVALEESLNLVTIRVAQYIGMRAIANT
                     AEAFGEVTDMPLVLPAAIGAVQTTVLQEAGAYAALAEEGRLVKPSLIDSVQTSDGTVV
                     WRPSGLSCDGCGDPAQPPRIVDQRKQIADPASVYQVIRMMEGVVKHGTGVPAVKGLNQ
                     PIAGKTGTTQNWVDAWFSGFTPGLVTVVWVGFDNPRTLGNGEQGAAVAAPIWHDFMVQ
                     ALKGQPPVDFPQPPGVTLASWDSSRGTRTDAFKPGQTPGASLPLYEGMVASSTPTPGS
                     PGAPPGQDASGTMPAASAQPEQGGGVDASLGGLY"
     gene            complement(6622..7494)
                     /locus_tag="VFN42_07985"
     CDS             complement(6622..7494)
                     /locus_tag="VFN42_07985"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019091013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET8996592.1"
                     /translation="MTASPISRRLLLGRLGSGAGIVGTFLLPPALHQAMAAPHHPYPH
                     RRTTAKPRAPLVMLDPGHGGKDPGAIGVSGTYEKHVSLAAAGELKRLLEAGGHYRVAM
                     TRTRDVFIPLEHRVAKAQEAGAALFVSMHADALSDHAVRGASVYTLSATASDAQTAAL
                     AQRENSADRFIGRTYRGVSPEVQRILASLARRETRVGSARIAGDLVHQLDREVLMLDR
                     PERHAGFVVLQSAEIPSVLVEMGFMSNPRDEAALRQAGHRRQIAIAMKRAVDGYFAAT
                     AHMSATLHGEWMAG"
BASE COUNT         1216 a         2809 c         2709 g         1365 t
ORIGIN      
        1 ccgcgctcag tcagcacgcc gaggatcgca tcgccttcgg tggcgcaggt gtccttgacc
       61 accaccagcc cgacattctc atcccgcatg cggcgggctg cggtaagcac ggggtcggtc
      121 atacggacgg aaaccacgcg atgatgtttg gcgcgcaagg cggcttcgat acgcattgga
      181 ccctcccggg ggtttgccgg gctcgggcgc ggatcggccg gagaccggac gttggcatac
      241 caaaattggc gttcggcgtt ttttatctgg tatatggtat gccaggaggc ctaggccgag
      301 tcaagcgacg ccggcgggct cgtgacccct cgccgctacc catcgccaag ccccgcacgc
      361 ggtactagtc tgcccggcat ggacgatctg cccgccgccc caatcagcaa catcccggaa
      421 ttcaccgtct cggaaatctc cggtgcggtg aaacgcaccc tggaaggcac cttcggccgc
      481 atccgggtgc gcggcgagat caccgaactg aagcgctacc cctccggcca catctacttc
      541 tcactcaaag atgagaacgc aaaattggcc ggggtggttt ggaagtccgc cgtcccccgg
      601 ctgggcatgg caccggaaaa cggggtggag gtgatcgcca ccggacggat ctccgcctat
      661 ggcgaacgtt cgtcatacca gctcatcgtc gagcggatgg aatacgccgg cgccggcgcg
      721 ctgctggccc ggatcgaaat gctgcgggta cgcttggccg ccgagggcct gttcgacacg
      781 gaccgcaagc gcaaactgcc cgtgctgccc gcggtgatcg gcgtggtcac cagcgagcgc
      841 ggtgcggtga tccaggacat ccgcaccact ctggcccggc gctttccccg cacgctgctg
      901 ctctggccgg tgccggtaca aggcgagggt tcggcggagc gtatcgccgc ggcaatcgcc
      961 gggttcgacg cactatcgcc cggcggcgcc gtgccgcgtc ccgacgtgct gatcgtcgcg
     1021 cgtggcggtg gcagcctgga agacctgatg gcgttcaacg aggaaatcgt cgtccgcgcc
     1081 gccgctgctt gccgcattcc gctgatctcc gcggtcggcc acgaaaccga caccacgctg
     1141 atcgacttcg cctccgatcg ccgggctccc acgcccaccg ccgcggcgga actggcagtg
     1201 ccggcccgcg cggacctgat cgccgacctg gcacagcgcg aggcgcggct ggtgggggcg
     1261 ctcaaccggc tgactcagga acgccgcctg cggctctccc gcgcggaacg cggcctgccc
     1321 gatctgccta ccctgctcgg caccgcccgg cagcggctgg acgaccgggc agagcggttg
     1381 cggctggcgc tgcctaatct cgtggagcgg cgccgcgccg ccctggtcgc ggcggagcgc
     1441 cggctgcctg atctgccggc gatgctgcat gcgcgccggg agcgggtgca cgaacgcggc
     1501 gtccgcctcg tgctggcctt gcccaacctg accgccgcgc gccggtcgtc gctggaattg
     1561 accagccacc agctcatcgc ctgtctgcgc catcgggtcg cggcgatcca caacagcgcc
     1621 aaccgcaccc tcgcccggct gaccggcgcg ccgctgcgat caaccctgcg cgaggcgcgg
     1681 gctcggctgg atggcgccgg tggccggctg gaggcgatgt cgccgctggc cgtgctgcaa
     1741 cgcggctacg tggtggtgtc cgattcggcc ggtcacaccc ttaccaacgc tgaggccgtg
     1801 aaaccgggag cccggctccg cttgcgcttc tgcgacggcg agctaggcgc taccgccgac
     1861 ggcggccgga aatcggaccg acagggccag ttgccgctgt aaccaatccg ggccggagtg
     1921 cggtgggcat taccgctccg ccgtttcggt gcttcctcca ccggcaagcg ccttggtggt
     1981 gcgcgtcgcc tgctcgacct gcttcggcgg caggcccgcg gcgccggcgc cgagcttcac
     2041 cttgccatcc ggcagcggca cggcgatcgg tttcaccacg accggtgcca cgccgacctt
     2101 cttcaaacca agctcattgg ccactttcgg gctgacatcg acaacccggc catcaacgaa
     2161 ggggccgcga tcctcgatct tcacggtggc cgacttgccg ttcttcagat tgaccacctt
     2221 ggcggtggtg ccaagcggca gggtcttgct ggcggcgatg ttcgaatgcg ggttcatacg
     2281 atcgccattg gccatcagct tgtgggcgaa acccggcccg taccaggatg ccttgccctt
     2341 ctgcttgcgt ccggaccgat cgatcgatgc cttgccgtgc ggcaccagcg gtgggagttc
     2401 gtccaggcgc ctggcttctt gctgcgcctg cagggaattc gccgggggga tactggcacg
     2461 cgccggcagg ctttcagtca ggatccatgc ggagcagaag atcattccaa gacgcaacag
     2521 agcaattcgc ccgagcgccg gcatcgcgac gtccttccaa agttggtgca cccgggggtg
     2581 ttggacgctg cgaacggcct gcgggttcgc ccggaacctg gtcagaattc cgcgcgcccc
     2641 actcgcgcga tccgtcggac gcgatttcgc ggcaggacga ggaccggacg gcgagcaagt
     2701 gccgcagcgc cccgttcgga gccggccatg acacggcagg tgattcggcc ctgatcggcg
     2761 gaaccgccgc ggatacggct taccgcgcct gggcgctggc ctcgctgcgg gtggcgcctg
     2821 cgcgggcggc cagggccgct gccatgaacg ggtccaggtc gccgtccagc acggcgtccg
     2881 gattgccctt ctccaccccg gtgcgcaaat ccttcaccag ctggtacggg gccagcacat
     2941 agttgcggat ctggtggccc cagccgatat cggtcttggc gctctccacc gcggcagcgg
     3001 cggcttcgcg cttctgcagc tccgcttcgt acagccgcgc cttcagcatc gccatcgcgg
     3061 tggcgcggtt gcggtgctgg ctgcggtcgg tctggcacgc gacgatgatg ccggtcggca
     3121 tgtgggtgat acggatcgcg ctttccgtct tgttgacgtg ctgcccgccg gcgcctgagg
     3181 cacgataggt gtccaccttc aggtcgctcg ggtcgatctc gatgtcgatg ttgtcatcga
     3241 ccaccggata gacccagacc gacgcgaagc tggtgtgccg ccgcgccgcg gaatcgaagg
     3301 gcgagatgcg caccagccgg tgcacgccgg cttcggtttt caaccagcca taggcgttgg
     3361 gaccgctgac cagcagcgtg caggacttga tgcccgcctg ctcgccctcg ctttcctcca
     3421 tcacctgcac tttgtagccg tgctgctccg cccaacgcag atacatacgc atcagcatct
     3481 cggcccaatc ctgggcctcg gtgccgccgg tgccggcatt gatctcgaca taggcgtcgt
     3541 tggcatcggc ctcgccggcc agcaggctct cgatctcccg ccgcttcgct tcctcggcca
     3601 gcgcgcgcag cgactccatg ccgtcggcga ccagcgcctc gtcactgtcc gcctcggcga
     3661 gcgcgatcag ttccagcgtg tcgctgacgt ccgcttccag cttgcgcaca ccgtcgatct
     3721 gcgtagccag caggttccgc tcgcgcatca gcttttgcgc cgcggcgggg tcattccaca
     3781 ggttgggatc ttcggcgcgg tggttcagtt ccgccaggcg gccttcggcg acatcccagt
     3841 caaagatgcc tcctcagcag cgcgacggac tgcttgatct gttcgttcag ggcgtcggat
     3901 tcggctgaca tgatacccgg ctagatagga cagggagcgg ctcaatacag cccgcccagg
     3961 gaggcgtcca caccgccgcc ctgctcgggc tgggcgctgg cggccggcat ggttccagag
     4021 gcgtcctgcc ccggcggtgc acccggggag ccgggtgtgg gcgtcgaact ggccaccatg
     4081 ccctcataga gaggcagcga cgcacccggg gtctggcccg gcttgaacgc atcggtgcgc
     4141 gtgccgcgcg acgaatccca gctcgccagg gtcacgccgg gtggctgcgg gaaatcgacc
     4201 ggcggctgtc ccttcagcgc ctgcaccatg aaatcgtgcc agatcggcgc agcgaccgcc
     4261 gcaccctgtt cgccattgcc cagggtgcgc gggttgtcaa agccgaccca gaccacggtc
     4321 accaggccgg gagtaaagcc ggagaaccag gcatccaccc agttttgcgt ggtccccgtc
     4381 ttgccggcga tcggctggtt cagccccttc accgcgggaa ccccggtgcc gtgcttcacc
     4441 acgccttcca tcatgcggat cacctggtag acgctggctg gatcggcgat ctgcttgcgc
     4501 tgatcgacga tgcgcggcgg ctgggccgga tcaccgcacc cgtcgcagga caggcctgag
     4561 ggccgccaaa ccacggtgcc atccgaagtc tgcacgctgt cgatcaggct cggcttcacc
     4621 agccggccct cctcggcgag cgcggcatag gcgccggcct cctgcaagac cgtggtctgc
     4681 acggccccga tcgctgccgg cagcaccagg ggcatgtcgg tcacttcgcc gaaagcctcg
     4741 gcggtgttgg caatcgctcg catgccgatg tactgcgcca cgcggatggt caccagattc
     4801 agggattcct ccagggcaac gcgcagcgaa gtcgggccgc cgaaggtgcc ctcatagttg
     4861 cccggccgcc atttcccctc gggggtatcg agcaccaccg gggcgttcag gaagcgctga
     4921 ctgggcgaca ggcctttttc catcgcggcc agatagacga aaggcttgaa gctggagccg
     4981 ggctgccgct gggcctgcgt ggcacggttg aactggctca tgtcgtagct ccagccgccg
     5041 accatcgcca gcacgcggcc ggtggtggga tccatcgaca ccagcgcgcc ttgcaccttg
     5101 gggatctgcc gcagcgccac cttcatcggg ccggcctgcc gagccggctc ggccgggtcc
     5161 gcttggatca tgatcacgtc gccgtcctgc accacgtcgg tgatgcggcg cggtcgcggc
     5221 ccgagcctgt cgtcatgtgc agcccgcgcc caggccacgt ccgacagcgg cagcacgccg
     5281 gtgcgcggct gcggctgggc gttcggctgg ctctccggcg acagccagcc aaccttcgcc
     5341 tcggtattgc tggtgctcag caccacggcc agccgccaat cgcccagcat gccggcgggg
     5401 gcggagactt tggccaaggc agcgggccag tcctgcgccg agccgacagc cagatgagtc
     5461 accggcccgc gccagccacc gcgccggtgg tcgtacacca tcagtccgtc acgcaccgcc
     5521 ttctcggccg cgttctgcag tttcgggtcc aggctggtgt gcaccatcag cccgccctcg
     5581 gtggtcacgt cattcccgaa cttggcgacc agctcgcggc ggacttcctc ggtgaaccac
     5641 tcggcgccgg gaatcggcgc tgggtgatga aactcggttg gcaggatcgg ctgggccttt
     5701 gcggcggccg cctgggccac cgtgatgttg tggtcatccg ccatgcggtc cagcacccag
     5761 tcgcgccgtg ccttcgccgc ttccggatat ttccacgggt tgtagttgtt cggcgccttc
     5821 ggcaacgcag caaggaaggc gtcctgcgcc aggtcgagct ggtccagcgg cttgttgaaa
     5881 tacgcctcgg ccgccgcggc cacaccatag gagcccatcc cgagatagat ctcgttcaag
     5941 tacagctcca ggatgcgctg cttgctcatc acctgctgca tgcggatggc cagcagcgcc
     6001 tcacgaacct tgcgcgacag cgacacccgg tcgttcagca gcatgttctt gacgacctgc
     6061 tgcgtgatgg tggaggcgcc gagcggtcgg cggccggtgc cgatgcgcat caggtcggta
     6121 aggccggcgc gcacgatggc cgccggatcg atgccaccat gcacccagaa attctggtcc
     6181 tcggcagaga cgaacgcctc ctgcacccgc ttcggaatgg cgttgatcgg catgaagatg
     6241 cgccgttccg ccgccaggtc cgccagcagc cggtcattgc cggcgaacac ccggctcatc
     6301 tcctttggct ggtagttctt caggccatcg acgctgggaa ggttggcgct gaagtgcagc
     6361 cacagaaagt acgcgccgcc ggaaccgacc accaggccga gcagcatgag gccgaggaac
     6421 gcaaccagca gtccacgcat cacgcgagaa aagcggcccc gcggcggctt ggacttcttg
     6481 gcagcgggcg gattcggctg atggtggtga tgatggtggt gtggccggcc gtgcggtgac
     6541 agcggctcac ccgctttcaa aggtccagtc atggcggcgc ggtatgatcc ctctgccgga
     6601 tagcgaccag cacgctttgc gctagccagc catccattcg ccgtggagtg tggcgctcat
     6661 atgggcggtc gcggcgaaat acccatcaac agctcgtttc atggcgatcg ctatctgccg
     6721 cctgtggccg gcttgccgca gtgcggcttc gtcgcgcggg ttggacatga accccatctc
     6781 caccagcacg ctggggattt ccgccgattg tagtacgacg aagccggcat gccgctccgg
     6841 gcggtccagc atcagcacct cgcggtcgag ttgatgcacc aggtcaccgg caatgcgggc
     6901 ggatccgacc cgggtttcgc gccgcgccag gcttgccagg atgcgctgta cttccggcga
     6961 aacgccgcga taggtgcggc cgatgaagcg gtcggcgctg ttctcccgct gcgccagcgc
     7021 ggcggtctgc gcgtcggaag cggtggcaga cagcgtgtag acgctggcgc cgcgcaccgc
     7081 gtggtccgac agcgcatcgg catgcatcga gacgaacagc gccgcgccgg cttcctgcgc
     7141 cttggcgacg cggtgctcca gcgggatgaa cacgtcgcgg gtgcgggtca tcgccacgcg
     7201 ataatggccg ccggcctcca gcagacgctt cagttctccg gcggcggcaa gcgagacgtg
     7261 cttttcgtac gtgccggaga cgccgatggc gcccgggtcc ttgccaccat ggcccgggtc
     7321 cagcatcacc agcggggcgc gcggcttggc ggtggtcctg cggtgcggat acggatggtg
     7381 cggcgccgcc atggcctggt ggagcgccgg cggcagcagg aacgtgccga cgatcccggc
     7441 ccccgatccg aggcggccga gtagcaggcg acgcgagatc ggcgaagcgg tcatgctggg
     7501 tccctaacac agcgactcgg gaatgtcagc ttacatgttg atctatcatc cctctccccg
     7561 ggcggcggcg gcacaggagt aaaccttgca gcccccggcg ctttgcggca tggtcgccga
     7621 cgctccggtg gcgcaatcgg gggtggccgc ggattctgcc gccccccggt ccgaatcggt
     7681 ggagtcgagg tcccgggaat aggccaaccg tgtgcaaaca ccggcaggcc ttagggggag
     7741 ggcccgctcc gccaaccgcg tcaccgcccg ccgcgcgcct gctttggcgc gccggggctg
     7801 cccggttggg tggccggcaa gcgttttttc tcacggggct gcgttccgca cgagcgcgcc
     7861 gtccggatgc cagggacaga tgaccgcaat tttgccggaa cctgccatgc ccgttgcacc
     7921 ccactcgatc ggcggcatgg ccggtgcagg cgctttgcgt gatgtcgatt ggccgggacg
     7981 agcgacccgc ccgggcgggc ccctttccgc aagcctattc agccgcgcac cggcgtgcgc
     8041 cccgtccggg accgcgccgc ctgctgccgc ggcgcaccgg agttcaggac tgaatgacc
//
LOCUS       DASSZH010000366         3164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_911830, whole genome shotgun sequence.
ACCESSION   DASSZH010000366 DASSZH010000000
VERSION     DASSZH010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911830"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..925)
                     /locus_tag="VFN42_07990"
     CDS             complement(125..925)
                     /locus_tag="VFN42_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HET8996593.1"
                     /translation="MLWHVLKPCTAAMIEAAPRLRLIQKIGVGVNTIDQEAARARGIP
                     VCNLPGTNARAVAELTLALMLATLRRLPRFDASLRQGTWSDPLLQDGLGELGGRTVGL
                     VGYGAIPRLLAPVLVALDCRVIYTARTPHEDAVGTWRPLDALLAEADVVSLHLPLTPQ
                     TTGLIDAAALARMKPGAILVNTARGGLIAQAALVNALRDGRLAGAGLDVFAQEPPDAA
                     DPLFGLPNVVLAPHIGWLTTGTFDRSFSLAAENCRRLQSGEALLHRVA"
     gene            complement(1054..2343)
                     /gene="eno"
                     /locus_tag="VFN42_07995"
     CDS             complement(1054..2343)
                     /gene="eno"
                     /locus_tag="VFN42_07995"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET8996594.1"
                     /translation="MTDTSIAFVHGRRVWDSRGRPTVEADVLLEGGAVGRAIAPAGAS
                     TGTGEALDLRDGGEAFGGYDVKRAVANVNGEIARAVLDMDAADQAALDRKLMDLDGSP
                     SKSRLGANAILAVSMAAAHAAAAASGMPLYRYLGGPDAGLMPLPQIQIFGGGAHAGRR
                     VDIQDFMVMCPAAQSFAEALDWTAEVYRAAGKLMQDSGTLAGVADEGGWWPAFATNEQ
                     ALETLVRAIERAGFQPGTQVGIALDIAASEFGRGGQYKLALEDKELDSEAMVKLLTGW
                     IRRYPILSIEDPLAEDDAPGFAAFTAAVGKQVQVVGDDFLVSQAPRVREAAVRGAANT
                     VLLKPNQRGTLTECHEAWKAAQEVGFAGIVSARSGETEDTTIVHLAVGWGVGQLKVGS
                     FSRSERMAKWNEALRVEEALGPRAQFAGAAMLGRSRR"
     gene            complement(2340..>3164)
                     /locus_tag="VFN42_08000"
     CDS             complement(2340..>3164)
                     /locus_tag="VFN42_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996595.1"
                     /translation="DWHAPVDRNRYEARWMQQANAAVPGCAPALLGQDEASGLLAMEY
                     LPPDRHPLWKNQLRDGHADPDFAAAVGDRLGRIHAATARRPELAAAFPTDGIFYDIRL
                     EPYLVATGRRHPDLAQRLHALVAGTQANKRALVHGDVSPKNILAGPLGPVFLDAECAW
                     WGDPAFHVAFCLNHLLLKCLWTPSARAGFLACFETLAAAYLPHVAWEPAPELERRAAH
                     LLPGLFLARVDGKSPVEYITAEADRNRVRRVARFLLDAPPDRLRDISLAWTRELAA"
BASE COUNT          483 a         1140 c         1069 g          472 t
ORIGIN      
        1 gcgggacgca ctgctgcgca cattgcggca gttcatccgc tatttcaggg acgagctgca
       61 gggacacttc ccggacccgt tcgatcatcg gcaggcgctg accgcgcgat cctgacgcgg
      121 cgatctaggc gacgcggtgc agcaacgctt cgccggattg caatctgcgg cagttctcgg
      181 cagccagcga gaagctgcga tcgaacgtgc ccgtggtcag ccagccgata tgcggcgcca
      241 gcacgacatt gggcaacccg aacagcggat cggcggcgtc cggcggttcc tgcgcgaaca
      301 cgtcgaggcc ggcgccggcg agccggccat cccgcaacgc attcaccagc gcggcctgcg
      361 caataaggcc gccgcgtgcg gtattgacca ggatcgcgcc gggtttcatg cgcgcgagtg
      421 ccgcggcatc gatcagcccg gtggtttgcg gtgtcagcgg caggtgcagg ctcaccacgt
      481 cggcttcggc cagcagcgcg tcgagcggcc gccacgtacc cacggcgtct tcgtgcggtg
      541 tccgcgcggt gtagatcacg cggcaatcca gcgcgaccag caccggcgcc agcagccgcg
      601 ggatggcgcc gtaacccaca aggccgacgg tgcggccgcc cagctcgccc aatccatcct
      661 gcagcagcgg atcggaccac gtcccttggc gcaatgaggc atcgaaccgg ggcagacggc
      721 gcagcgtggc cagcatcagc gccagcgtca gttcggccac cgcgcgggca ttggtgccgg
      781 gcaggttgca gaccggaatg ccgcgcgccc gcgccgcctc ctggtcgatc gtgttcacgc
      841 cgacgccgat cttctggatc agccgcagcc gcggcgcggc ttcgatcatt gccgcggtgc
      901 agggcttcag cacatgccac agcacgtcgg tttacggcag cagccgggta agcagcgcat
      961 cgtcgctgtc cgggcaaacc gcaatgtcca gcccgggcac cgccgccagt cgtgccgcga
     1021 gatcaggccc cgcgacagcg tggaacagca ccctcaccgt ctggaccgcc ccagcatcgc
     1081 agcaccggcg aactgcgcac gtgggccaag cgcctcctcc acgcgcaggg cttcgttcca
     1141 cttcgccatg cgctcgctgc gggagaagct tcccaccttc aattgcccga cgccccagcc
     1201 gaccgccagg tgcacgatcg tcgtgtcctc ggtctcgccg gagcgggcgg acacgatgcc
     1261 ggcgaagccc acctcctgtg ccgccttcca ggcctcgtga cattcggtca gcgtgccgcg
     1321 ctggttgggc ttcagcagca cggtgttggc cgcgccgcgc accgcggctt cgcgcacgcg
     1381 cggcgcctgg ctgaccagga agtcgtcgcc gaccacctgc acctgctttc ccaccgccgc
     1441 ggtgaaggcg gcgaagccgg gcgcgtcgtc ctccgccagc ggatcctcga tcgacaggat
     1501 cgggtagcgg cggatccagc cggtaagcag cttcaccatc gcctcgctgt cgagttcctt
     1561 gtcctccagc gcaagcttgt attggccgcc gcggccgaat tccgaggccg cgatgtccag
     1621 tgcgatgccg acctgcgtcc ctggctggaa cccggcgcgt tcgatcgcgc gcaccagggt
     1681 ctcaagcgcc tgttcgttgg tcgcgaaggc cggccaccac ccgccttcat cggcaacccc
     1741 ggccagcgtg ccggagtcct gcatcagttt gcccgcggcc cgatagacct ccgcggtcca
     1801 gtccagggcc tccgcgaagc tctgcgcggc cgggcacatc accatgaaat cctgaatgtc
     1861 gacgcgccgg cccgcgtggg cgccgccgcc aaagatctgg atttgcggca gcggcatcag
     1921 cccggcatcg gggccgccga gatagcggta cagcggcatt cctgacgctg ccgccgccgc
     1981 gtgcgccgca gccatcgaga ccgcgagaat ggcattggcg ccgagccgcg atttgctggg
     2041 ggagccatcg aggtccatca gcttgcggtc cagtgccgcc tggtccgccg cgtccatgtc
     2101 gagcaccgcg cgggcgatct cgccattgac gttcgccacg gcgcgtttga cgtcgtagcc
     2161 gccgaacgct tcgccaccgt cccgcaggtc caacgcctcg cccgtgccgg tggaggcgcc
     2221 cgccggtgcg atggcccgcc ccaccgcgcc gccttccagc agcacatccg cttccaccgt
     2281 ggggcgccca cggctgtccc acacccggcg gccatgcaca aaggcgatgc tggtgtcggt
     2341 catgcggcga gttcccttgt ccatgccagg cttatgtcgc gaagccggtc cggcggcgcg
     2401 tccagcagga aacgcgcaac gcggcgcacc cggttccggt ccgcctcggc ggtgatgtac
     2461 tccactggcg acttgccatc cacccgggcg aggaacaacc cgggcagcag gtgtgccgcg
     2521 cgccgttcca gttcgggcgc gggttcccag gcgacatgcg gcagataagc ggcggcgagc
     2581 gtctcgaaac aggccaggaa gccggcgcgg gcggacggcg tccacaggca tttcagcaac
     2641 aggtgattga ggcagaacgc cacgtggaag gcgggatcgc cccaccacgc gcattccgca
     2701 tccaggaaca ccgggccgag cggtccggca aggatgttct tcggactcac gtcgccatgc
     2761 accaaggcgc gcttgttcgc ctgcgtaccg gccaccagcg cgtggaggcg ctgtgccagg
     2821 tcgggatgcc tgcgcccggt cgcgacgagg tatggctcca gccggatgtc gtagaagatg
     2881 ccgtcggtgg ggaacgcggc ggcgagttcc gggcggcgcg cggtggcggc atggatgcgg
     2941 cccagccggt caccgaccgc ggcggcgaaa tccgggtcgg catggccgtc gcgcaactgg
     3001 ttcttccaca gcggatgcct gtccggcggc aggtactcca tggccagcag gccggatgcc
     3061 tcatcctggc cgagcagggc gggggcgcag cccggcactg ccgcgttggc ctgctgcatc
     3121 catcgtgctt cgtagcggtt gcgatcgacc ggcgcgtgcc agtc
//
LOCUS       DASSZH010000367         3183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_468782, whole genome shotgun sequence.
ACCESSION   DASSZH010000367 DASSZH010000000
VERSION     DASSZH010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468782"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VFN42_08005"
     CDS             complement(<1..215)
                     /locus_tag="VFN42_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET8996596.1"
                     /translation="MKSVKVLGPGCKRCVATAEMVQAEADRLGISVTIEKVTDYAAIA
                     GYGIAATPGVVVDGKVVHAGGLPKPDNV"
     gene            complement(232..1293)
                     /locus_tag="VFN42_08010"
     CDS             complement(232..1293)
                     /locus_tag="VFN42_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HET8996597.1"
                     /translation="MSTATLTAPPRKRLSGNARLIGATALALVAWVGVYWQLAWFSSW
                     LVAKLPIPHGGHLDEALSFFIFDTPKVLMLLALVVFAMGVLRSFFSPERTRAVLAGKR
                     EGLGNAAAAALGIVTPFCSCSAVPLFVGFVSAGVPLGVTFSFLIASPMVNEVALGLLF
                     ALVGWQVALIYLVFGLALAMIAGWVIGRLHVESWLEPWVLEVRSGTAELTAADQTIAD
                     RIHAGIAAMRDIFGRVWIWIILGIAAGAFIHGYVPADMLAGVMGSHHWWSVPAAVLIG
                     VPIYSNAAGIIPVVEALLGKGAALGTVLAFMMAVTALSAPETIILRKVLTIRLIAVFV
                     GVVAIGIMAIGFLFNALFA"
     gene            complement(1290..1586)
                     /locus_tag="VFN42_08015"
     CDS             complement(1290..1586)
                     /locus_tag="VFN42_08015"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET8996598.1"
                     /translation="MTQALNILAALAEPTRLAAIQLLWDGHEHCVCELMDRLGATQSR
                     MSRHMAALKAAGLVVDRRDAQWVRYRRAPNLPAGAERLVDAALSLAAQSDRIAA"
     gene            complement(1732..2793)
                     /gene="arsB"
                     /locus_tag="VFN42_08020"
     CDS             complement(1732..2793)
                     /gene="arsB"
                     /locus_tag="VFN42_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HET8996599.1"
                     /translation="MEQLIQQAPPRPRLGVFERYLTLWVALCIVAGIALGSIAPAPFH
                     TLGALSVASVNLPVAVLIWLMIVPMLLRVDFGAIGTVTRHWRGIVVTLGINWLVKPFS
                     MALLGWLFIGGLFHTLLPADQINSYIAGLILLAAAPCTAMVFVWSNLSDGDPNFTLSQ
                     VALNDAIMILAFAPIVGLLLGLSAITVPWDTLFLSVVLYIIVPLIAAQLWRRRTLSAG
                     GEPKLARLLRALHPVSLLALLATLVLLFGFQGEQILAQPLVILLLAVPIVIQVYFNAG
                     LAYLLNRACGSAHNVAAPSALIGASNFFELAVAAAISLFGFQSGAALATVVGVLVEVP
                     VMLSVVHLVTATRGWYARG"
     gene            complement(2865..>3183)
                     /locus_tag="VFN42_08025"
     CDS             complement(2865..>3183)
                     /locus_tag="VFN42_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8996600.1"
                     /translation="DPSLWRPDIVLVIALSIFGLPFAVNSSLHSYLILAYAGSEKAAE
                     DVGFYYAANAAGRLLGIMLSGGLTEAGGMPACLWGSAIMLAICLAITVLLPSGAEACR
                     VAA"
BASE COUNT          621 a         1036 c         1090 g          436 t
ORIGIN      
        1 acgttgtccg gcttcggcag gccgccggca tgcaccacct tgccgtcgac caccacgccc
       61 ggtgtcgcgg cgatgccata gccggcaatc gcggcgtagt ctgtcacctt ttcgatggtg
      121 acggagatgc cgagccgatc ggcctcggcc tgcaccatct cagcggtggc cacgcagcgt
      181 ttgcagccgg ggccgagtac tttcacgctt ttcattgcat gtctccttgg gtcaggcgaa
      241 cagggcgttg aacaggaagc cgatcgccat gatgccgatc gcgacgacgc cgacgaagac
      301 ggcgatcagc cttatggtca gcaccttgcg caggataatc gtctcgggtg cggacagggc
      361 ggtgaccgcc atcatgaagg ccagtacggt gcccagggcc gcgcccttgc caagcagcgc
      421 ttcgacgacc ggaatgatgc cggccgcatt ggaatagatc ggtacgccga tcagcaccgc
      481 cgccggcacc gaccaccagt gatggctgcc catgacgccg gccagcatgt ccgccggcac
      541 gtagccatga atgaatgcgc cggccgcgat tcccaggatg atccagatcc acacgcggcc
      601 gaagatgtcg cgcattgccg cgatgccggc gtggatgcgg tcggcgatcg tctggtcggc
      661 tgccgtcagc tcggccgtac cgctgcggac ctccaacacc catggttcca gccagctttc
      721 cacgtgcagc cggccgatca cccagccggc gatcatcgcc agggcaaggc cgaacacgag
      781 atagatcagc gccacctgcc agccgaccag ggcgaacagc aggcctagcg ccacttcgtt
      841 gaccatcggc gaggcgatca ggaaggagaa ggtgaccccg agcggcacgc cggcggagac
      901 gaagccgacg aacagcggca ccgcggagca ggagcagaac ggtgtgacga tgcccaaagc
      961 ggcggcggcg gcgttgccca ggccctctcg cttgccggcc aggacggcgc gggtgcgttc
     1021 gggcgaaaag aagctgcgca gcacgcccat ggcgaacacc accaaggcca gcagcatcag
     1081 cactttcggc gtgtcgaaga tgaagaagct cagcgcttcg tcgagatgtc cgccatgcgg
     1141 gatcggcagc ttggccacca gccagctgga gaaccacgcc agttgccagt agacgccgac
     1201 ccaagcgacg agggccagtg ccgtcgcgcc gatcaggcgg gcgtttccgg acagccgttt
     1261 ccgcggcggc gcggtgaggg ttgccgtgct catgcggcga tccgatccga ttgcgctgcc
     1321 agcgacagcg cggcgtccac caggcgttcg gcgccggcgg gcaggtttgg cgcgcggcgg
     1381 tagcgcaccc actgggcatc gcgccggtcc accaccagac cggccgcttt cagtgcggcc
     1441 atgtggcgtg acatgcgcga ctgggtcgca cccaggcggt ccatcagctc acagacacag
     1501 tgttcgtgcc cgtcccacag cagctggatg gcagcgaggc gggtcggttc ggccaaggcg
     1561 gccaggatgt tcagggcttg ggtcatgcgc gccatatgcg tagtagagca tatgcgtgca
     1621 agcggatatg ctatgcggca ggcacggatg agcgttgcgg gcggcaggag gttgccgccc
     1681 gcattgccgc cccttctggt caaagggtga gcaggggcgc ggaaggcgat ctcagccgcg
     1741 cgcgtaccag ccgcgcgtcg cggtaaccag atgcaccacc gacagcatca ccggcacttc
     1801 caccagcacg ccgaccacgg tcgcgagggc ggcgccggac tggaagccaa acaggctgat
     1861 cgcggcggca acggccagtt caaagaagtt gctggcgccg atcagcgccg agggtgccgc
     1921 gacgttgtgc gccgaaccgc aggcgcggtt gagcagatag gccaggccgg cgttgaaata
     1981 cacctggatg acgatcggca ctgccagcag caggatgacc agtggctggg cgaggatctg
     2041 ctcgccctgg aagccgaaca gcagtaccag cgtggccagc aacgccagca gcgacacggg
     2101 atgcagcgcg cgcagcaacc gtgccagttt cggctcgcca cccgcggaca aggtccggcg
     2161 gcgccacaac tgcgcggcga tcagcggcac gatgatatac agcacgaccg acaggaacag
     2221 cgtgtcccag ggcacggtga tggcggacag gccgagcagc aggccgacga tcggcgcgaa
     2281 ggcgaggatc atgatggcgt cgttcagtgc cacctggctc agcgtgaagt tgggatcgcc
     2341 gtccgacaag ttggaccaca cgaacaccat ggccgtgcag ggcgcggcgg ccagcaggat
     2401 caggccggcg atatagctgt tgatctgatc ggcgggcagc agtgtgtgga acagcccgcc
     2461 gatgaacagc cagcccagca gcgccatgga gaagggcttg accagccaat tgatgcccag
     2521 cgtgacgacg atgccgcgcc agtggcgggt gaccgtgccg atggcgccga aatccacgcg
     2581 cagcagcatc ggcacgatca tcagccagat cagcacggcc accggcaggt tgacgctggc
     2641 gacgctgagt gcgccgagcg tgtggaacgg cgccggtgcg atactgccca gcgcgatgcc
     2701 ggcgacgatg cacagcgcca cccacagtgt cagatagcgt tcgaagacac caagccgggg
     2761 acgcgggggc gcttgttgaa tgagctgttc cacgaagctt cctctggttc gaaacacggg
     2821 cgcatcaccg cgggccgcgg cgatgcgcat cactcccgca gccttcaggc agccacccgg
     2881 catgcctccg cgccggaggg tagcagcacg gtgatcgcca ggcagatcgc cagcatgatc
     2941 gccgatcccc acaggcaggc cggcatgccg ccggcctcgg tcaacccgcc ggacagcatg
     3001 atgccgagca gccggcccgc ggcattggcg gcgtagtaga agccgacgtc ctcggcggcc
     3061 ttctccgacc cggcataggc caggatcagg taggaatgca gcgaggaatt gacggcaaac
     3121 ggcaggccga agatcgagag cgcgatcacc agcacgatat cgggacgcca caggctgggg
     3181 tcg
//
LOCUS       DASSZH010000368        11362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_162369, whole genome shotgun sequence.
ACCESSION   DASSZH010000368 DASSZH010000000
VERSION     DASSZH010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 11362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11362
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162369"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..1196)
                     /locus_tag="VFN42_08030"
     CDS             complement(201..1196)
                     /locus_tag="VFN42_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996601.1"
                     /translation="MEGDMTLTTLVRRSLRTLLLGSAAFCVTAAVNLPPAFFPGGSGA
                     AFAQGASSSGHGGAGGYRGGAGSKGGASGMHGSGEHESSAGEEEHGSTHGSTGGEGGH
                     DAAGGTGGPSGGASGGAAASRGHHGESGGEASGEGSSEESKGPHYGGGASARKPEGGS
                     TGGRPNWASEGIPETELGRLNVARAPASVLDHAFSEVTANWPTTRNTVITLNDGQQLT
                     VAQLYSKPAVEFARIVQNNYQAIVRIDSPLENLSLLKDVVTNHRTVLSGVTPASSTDL
                     AAIFLGSASDKTIPITTDTVTALYTILGMQVPQDAATLATEADAVRSGISIGHGE"
     gene            complement(1552..2805)
                     /locus_tag="VFN42_08035"
     CDS             complement(1552..2805)
                     /locus_tag="VFN42_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996602.1"
                     /translation="MTSRTPVRRGLRTILLGSAALCFAATLSLPPIGGSGSIGTAFAQ
                     SGQGKGGGGQGGGGQGGGGSAQARGGQGAAAAAQGSGQGQGGAGSTRGQGAGQGGGQS
                     SVEHGNRGSGTVGSGEDSDSDRPAWAGTKGGKSGGGTKPSEAGTKKGDTYGDLYVILR
                     DANGVPILNAAGFVQPLDASGNLIPLDAEGAPVDETLLVAVELARLSVGKSPDKVLDR
                     QYTEAIASLNAATSITTDDAGRLVVTNPDGTVATIDSPLANLALYKTLLSQGTLPGLT
                     ATDAVLGALSYLKDGQLTVADYKEAAALLAAASDKTVKVTVDTVVDMNTILGVTGTIT
                     GADGKTYVSFTNYTYDRAATYPGTVTVLVKNADGTYSKQDVSIMEAVFNNTAYEGTGA
                     TAFATAADDARAVILFVHDNPLPTE"
     gene            complement(2875..3423)
                     /locus_tag="VFN42_08040"
     CDS             complement(2875..3423)
                     /locus_tag="VFN42_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996603.1"
                     /translation="MKQAIDRQQAPPDRTRRRLLQFAVLMPIGLLLTAGAGWAQEHGG
                     GGESGGGHSGGGGESGGGHGGGGGESGGGHGGGGGESGGEEGGGGGQGAGRNAQQRAY
                     RHRQRTGRTLGGGEHGGGEHGGGGEHGGGHAGNEGSEPGLPPGATGTLSSGEIGGGTG
                     GEHFVHDSQGYWGADGKVLRRR"
     gene            complement(3452..3997)
                     /locus_tag="VFN42_08045"
     CDS             complement(3452..3997)
                     /locus_tag="VFN42_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996604.1"
                     /translation="MMRVRQQTVLHRTMRDRLAMRNGLLRAAVVTALFGTLGSSVLQL
                     GIAQAQPRTLEPRQDAPSQVYLDLNTWCADYRPGLSAGVPDSREQKRLQAVQTSLAPG
                     FDPGRRKDGIALLQAYRQVLEQRHPDRTLAATYLALTSTVPITFAVVERTNALLCVSS
                     APAFARAVADAAEAERREMAR"
     gene            4122..5723
                     /locus_tag="VFN42_08050"
     CDS             4122..5723
                     /locus_tag="VFN42_08050"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET8996605.1"
                     /translation="MLASSHRSAAPARDRRRRLGPIALLMLALAPAAGMAQPAPAQPI
                     LGERLNAAAVAGPTALLDTLSRELARNPRLAATPGSAATLARAAATPVQGFVGARGPV
                     YREIAQRIIAAAPAGQRGAVQQAVSEALRPYTEMPVRIALPQRSPLVVGTTRPAAPQV
                     GSLGIKLGSFTVYPDVQASTYYDDNIYATSHGHVSDWIGTISPRIAIQSNWDRHSLYA
                     EAGADLTGYWSHPHENSADWHVLSEGQIDVSDKTQILLGAIALKSHEDRASPDAVEGI
                     DPTPYNEEDGYLGVVHRFGDFTLRLGGAIEHLTFGNVLGTHGEINNEDRDRTRYTFGG
                     LLRYEANPKLRPFVEVMGDLRRYVTVPDDFGFNRNSDGFLAGVGTLYTLTDKLSGEVF
                     LGVLHRNYTDPSFKSLTTPAADATLRWQATGRTAVVLFTERTIEETTLFGSPGYIYTL
                     VGGRIEQRLTDRLTGILRAAYAHSDFAQAGRNDNDVDTSVGVRYRLTSRVTLGVDYRY
                     TQRVSGSTLTDFSRNQVFFRIGTEF"
     gene            complement(5766..6548)
                     /locus_tag="VFN42_08055"
     CDS             complement(5766..6548)
                     /locus_tag="VFN42_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8996606.1"
                     /translation="MEVRLDGRTAIITGASKGLGLAMATEFAASGADVAILARRPDAL
                     AEAKAIASKNARGRVETFSCDVAQADQITRTFDAVIKTFGKVDILVNNAGTSRAMPST
                     QITDEIWQEDLELKLFGAIRFVRLVWPGMQQRKWGRIISVLNLGAKAPRANGAPTAVS
                     RAAGMAFMKVLANEGAPHGILVNGLLVGLIDSDQHVRRHQQQGSNASYEEFRAKMGAN
                     VPLGRIGKAEEFAAMACFLCSDLAGFTTGAAINMDGGASPVV"
     gene            6704..8530
                     /locus_tag="VFN42_08060"
     CDS             6704..8530
                     /locus_tag="VFN42_08060"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET8996607.1"
                     /translation="MPTSQPRLCLLTPESTPPGPDCAGLFLALRKHATTAGYRVTEAA
                     VSETGIRILGPEQADPAPLPQRFFGPAAAQAAYRVLEWLRAEPFDIVCAPAEAGLLFY
                     ALAARHQAVALTGTTFCVLAERLTLLSRHRAGEMPRDMEDLVRDEMERRMLALPDAVL
                     VTDSGIAPWVREQGWTVAGLRDCGDTPQAAVQALWPDLLAAARRAAPPADADGDMPLV
                     SVCIPHFNRPTLLWQAVSSIRAQTYPRIELVVVDDASTDPAALATLDTLEAELAEQGG
                     KLVRHPVNRYLGAARNTGARHASGAFVLFLDDDDYAKPEQVATLVRAAQATGADIVNS
                     LCERLQGQEPPKPGQQSEERWLTLGNVPTLGLFRNLFGTASALFRKTSFERLGGFTEM
                     HGIGCEDWEIFARASLAGMNLQTVPEALYWYRLTQGSMIQTGNVYAGESRAMAPYLAL
                     VPQVLWPALRFAHGAEQRLQTAQDSLRHGEAVLRHRDALIAHRERHIAHLTQLDRERA
                     EPLAALSREVAELRAVLRVTMGGYGPTPQSAVDSMLQSGSWRLGRLLQAPLRWMRGRP
                     ETAPPRVTDWVQAASVINSMQRSTTWEMTGLLRALAGLLRRR"
     gene            8587..8847
                     /gene="grxC"
                     /locus_tag="VFN42_08065"
     CDS             8587..8847
                     /gene="grxC"
                     /locus_tag="VFN42_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HET8996608.1"
                     /translation="MPEIEIYTQPWCPYCARALHILGEKGVAFREIHAPNGTPEREEA
                     RNRSGGRTSVPQIFIDGRHVGGCDDLVALDRAGKLDPLLHPA"
     gene            8942..9421
                     /locus_tag="VFN42_08070"
     CDS             8942..9421
                     /locus_tag="VFN42_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HET8996609.1"
                     /translation="MIHYQLRCSQQHEFDGWFKDSAAFEQQAKRGLVDCPVCGDVQVT
                     RALMAPAVTTREAAPPQPPQPAPQSPAAPAEAPPARMAGGKLPAHMVAMLQRLRSEVE
                     KNCDYVGPAFADEARKIHRGESEKRGIYGETTPDQAESLAEEGIEVARIPWVPRADG"
     gene            complement(9608..10318)
                     /gene="ubiG"
                     /locus_tag="VFN42_08075"
     CDS             complement(9608..10318)
                     /gene="ubiG"
                     /locus_tag="VFN42_08075"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HET8996610.1"
                     /translation="MSGATVSADEVARFDALAARRWDPDGPMRPLHRMNPARIGWICE
                     RIARRFPQHQGLRLLDLGCGAGLASEALARQGFDVLGLDAAGEAIEAAQAHAAGQHLT
                     LAYRSGTAEDLLAEGARFPAITALEVIEHVPDPAAFLHVLAGLLAPGGLLFLSTLNRT
                     PQSFLAAKVGAEYLLRWLPVGTHDWRKFITPAELASLLHGAGLRTADISGLQPDGLRG
                     GWRAVRDLSVNYLVAAEG"
     gene            complement(10635..>11362)
                     /locus_tag="VFN42_08080"
     CDS             complement(10635..>11362)
                     /locus_tag="VFN42_08080"
                     /inference="COORDINATES: protein motif:HMM:NF040572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HET8996611.1"
                     /translation="PGDIPNRGFLQPDITLHGLTYLQQVQDANVLGPNGEPAGIHVEP
                     GIWIHVPMTSNPQDPTTVARLANIPHGTSLIAQGTALPPINEAPHIDPVSITPFTIAP
                     PHSPVQFPESNLATRTEFRTDPEDIPRVTQEIVDNPNKFLKLGLQGKNVISTMTLKIS
                     TQPLKPPSSGGGISNIAFLQGAAGGPNASAAQMEAIFWLETIEEPDGRIVNQLQYTQT
                     VLLNFNNLSWPHVSVATLIKQPG"
BASE COUNT         1713 a         4056 c         3791 g         1802 t
ORIGIN      
        1 ctccagggcc acggactccg gagccataga ccgccggacc atggacgcca gggccacgga
       61 ctccagggcc agggactccg ggaccaggga ccgccgcacc agggaccgcc gccgccggcc
      121 gcctcggttg cgaccgaact gcggggcggc gccggcgtgc ctgccgcccc gagcctgcgt
      181 gtgccgcagc caggtcaagc ctattcgccg tggccgatac tgatcccgct tcgcaccgcg
      241 tcggcctcgg tggccagggt ggccgcgtcc tgcggcactt gcatgccgag gatcgtgtac
      301 agcgcggtca cggtgtcggt ggtgatgggt atcgtcttat cggatgccga tcccaggaag
      361 atcgccgcca ggtccgtgct gctggccggg gtcacgccgg agagcaccgt ccggtggttg
      421 gtgaccacat ccttgagcag actgaggttc tccagcggtg agtcgatgcg tacgatcgcc
      481 tgatagttgt tctgcacgat ccgggcgaat tcaacggcgg gcttggagta caactgcgcg
      541 accgtgagct gctggccatc gttcagcgtg atgaccgtgt tgcgcgtggt tggccagttc
      601 gccgtgacct cactgaacgc atggtccagt acgctggccg gtgcgcgtgc gacattcagc
      661 cggccgagtt cggtctccgg gataccctcc gaggcccaat tcggccgacc gcccgtgctg
      721 ccgccctccg gcttgcgggc gctcgcgccg ccgccatagt gcggcccctt gctttcctcg
      781 ctgctgcctt cgcccgacgc ttcaccgccc gattcgccat gatggccgcg cgatgccgcg
      841 gcgccgccgc tcgcgccgcc ggatggcccg cccgtgccgc cggctgcatc atgcccgcct
      901 tcgccgccag tgctgccatg agtgctgccg tgctcttcct cgccggcgct cgactcatgt
      961 tcccctgagc catgcatgcc ggaagcgccg cccttgctgc cggcgccgcc tcggtagcca
     1021 ccggccccgc cgtggccgga cgacgacgcg ccttgcgcga aggctgcgcc gctaccgcca
     1081 gggaaaaagg cagggggtag attcaccgcc gccgtaacgc agaacgcggc ggaaccaagc
     1141 agaagcgtcc gcaaactgcg gcggaccaga gtggtaaggg tcatgtcgcc ctccatgcgt
     1201 gccacccgca ttgcgccgcc cgcttgctcg ccgtcctgta ttccggagca accgccacca
     1261 cgcctggatc cgcgtggccc cggtgcccgc atggccgccg ttaccagcct tgccgccacc
     1321 gccatggccg gatgccgccg caggggccac gccaatgccc tgccggtgga ccggcgcgct
     1381 gggcgttggc gaaggtgagc ggcaatcgcc aggccggtgg tggcgccgac gcgacggaac
     1441 gccgagcata ccgggcagaa gcgcgtggca tgctcatcgc ggaactctgc tgtggcgcgg
     1501 cgattgcgga gaattcggcc ttcgcaatcg ccggccagaa cgttgccggc cttactcggt
     1561 cggcaacggg ttgtcgtgca cgaacaggat caccgcccgg gcgtcgtccg ccgcggtggc
     1621 gaaggcagtc gcgccggtcc cttcgtaggc tgtgttgttg aacaccgcct ccatgatcga
     1681 cacgtcctgc ttgctgtaag tgccgtcggc gttcttcacc aggaccgtga ccgtccccgg
     1741 ataggttgcg gcccggtcgt acgtgtagtt ggtgaagctc acgtatgtct tgccgtcggc
     1801 accggtgatc gtgccggtga caccaaggat ggtgttcatg tcgaccacgg tgtcgaccgt
     1861 caccttcacc gtcttgtcgg atgccgcggc caaaagggcg gccgcttcct tatagtcggc
     1921 caccgtcagc tggccgtcct tcaggtagct cagcgctccc agcaccgcgt cggttgcggt
     1981 gagacccggc agcgtgccct gtgacagcag cgtcttgtag agcgccagat tggcgagcgg
     2041 cgaatcgatc gtcgcaacgg tgccgtcggg attggtgacg acgagccggc cggcgtcatc
     2101 ggtggtgatg ctggtcgcgg cgttcaggct ggcgatcgcc tcggtgtact ggcggtccag
     2161 caccttgtcc ggcgacttgc cgacgctcag ccgggccagt tccacggcaa ccagcagggt
     2221 ttcgtcgacc ggcgccccct ccgcgtcgag cggaatgaga ttgccgctgg catccagcgg
     2281 ctggacgaac ccggcggcgt tcaggatcgg cacgccgttg gcgtcacgca ggatgacgta
     2341 gaggtcacca taggtgtcgc ctttcttggt gccggcctcc gagggcttgg ttccaccgcc
     2401 gctcttgccg cccttggttc cggcccaggc gggacggtcg gagtcgctgt cctcaccgct
     2461 accgacggtg ccggaacccc ggttgccgtg ctcaaccgaa ctctggccgc cgccttgacc
     2521 cgcgccctgg ccccgcgtcg acccggcgcc accctggcct tggcccgagc cctgcgccgc
     2581 cgcagcggcg ccctggccgc cccgggcctg cgcggacccg ccgccgccct ggccgccacc
     2641 gccctggccg ccgccacctt tgccttggcc gctctgggcg aatgcagtgc caatgcttcc
     2701 gctgccgccg atgggcggca gactcaacgt tgccgcaaag cagagcgcgg cggagccgag
     2761 cagaatggtc cgcaagccgc ggcggaccgg agttcgtgat gtcatgtcgc cctccttcgg
     2821 acgagagttt ccgaccaacc aaagcgtggc cggcccgccg ttccacgagg cgcgtcagcg
     2881 ccgccgcagt accttgccgt ccgcgcccca gtaaccctgg ctgtcgtgga cgaaatgttc
     2941 gccgccggtg ccgccgccga tttcaccgct gctcagcgtg ccggttgccc ccggcggcag
     3001 gccaggctcg gacccttcgt ttccggcgtg tccgccacca tgttcgccgc cgccgccatg
     3061 ttcgccgccc ccatgttcgc cgccgcccag ggtgcggccg gtgcgctggc gatggcggta
     3121 ggcgcgttgc tgcgcgttgc ggccagcacc ctgcccgcct ccgccgcctt cctcgccgcc
     3181 gctctcgcca ccgccgccgc cgtgaccgcc gccgctctca ccgccgccgc caccgtgacc
     3241 gccgccgctc tcaccgccgc cgccactgtg gccgccgccg ctctcaccgc cgccgccgtg
     3301 ttcctgcgcc catccggcgc cagccgtcag caacagcccg atcggcatca gcacggcgaa
     3361 ctgcagcaat ctccgccgcg tgcgatccgg aggggcctgt tgcctgtcga ttgcctgttt
     3421 catcgctccg cgccttccaa cccgccgcgg ctcaacgcgc catctcgcgg cgttcggcct
     3481 cggccgcatc cgccacggca cgcgcgaagg caggcgcgct gctgacgcac agcagcgcat
     3541 tggttcgctc caccacggcg aaggtaatcg gcaccgtgct ggtcagtgcc aggtaggttg
     3601 ccgccagcgt ccgatccgga tggcgttgtt ccagcacctg ccgataggcc tgcagcagcg
     3661 cgatgccgtc cttgcggcgc cccggatcga agccaggcgc caggctggtc tgcaccgcct
     3721 gcaagcgctt ctgctcgcgg ctgtccggta cgccggccga caggccgggc cggtaatccg
     3781 cgcaccaggt attcaggtcc agatacacct gcgatggggc atcctgccgt ggctccaggg
     3841 tgcgcggctg cgcctgggcg atgccaagct gcaacaccga tgagccaagt gtgccgaaca
     3901 gcgccgtgac caccgccgcc cgcagcagac cgttgcgcat cgccaatcgg tcgcgcatgg
     3961 tgcggtgaag taccgtttgt tgccgaacgc gcatcatgac catcaccttg tgggccttat
     4021 gcctagcgcg atccgcccgc cttttctttg atgtaaatca aagaaaagct gccccaacag
     4081 tgctcaattc cacgcgtctc atccgcgacc aggagccatc catgctcgct tcgtcacacc
     4141 gctctgcggc cccggcgcgc gaccggcgcc gccggctggg tccgatcgcg ctgctgatgc
     4201 tggcactcgc gcccgccgcc ggcatggccc agcccgcgcc ggcgcaaccc atcctcggcg
     4261 agcggttgaa cgcggcggca gtcgccggcc cgaccgcgct gctggacacc ctgtcccgcg
     4321 aactggcgcg caatccgcga ctcgcggcca cgccgggatc ggccgccaca ctggcgcgcg
     4381 ccgcggcaac gccggtccaa ggcttcgtcg gcgcgcgcgg cccggtctat cgggagatcg
     4441 cgcagcgcat catcgccgcc gccccggccg gccagcgcgg cgcggtgcaa caggcggtca
     4501 gcgaggcgct gcgcccgtac accgaaatgc cggtgcgcat cgccctgccg cagcgttcac
     4561 cgctggtggt cggcaccacc cggccggcgg caccgcaggt gggcagcctg ggcatcaagc
     4621 ttggatcgtt caccgtctat ccggacgtcc aggcctccac ctactatgac gacaacatct
     4681 acgcgacgtc gcacggccat gtctccgact ggatcggcac catttcgccg cgcatcgcca
     4741 tccaatcgaa ctgggatcgg cattccctct acgccgaggc tggggccgac ctgaccggct
     4801 actggagcca cccgcacgag aactcggccg actggcacgt gctgagcgaa ggccagatcg
     4861 acgtcagcga caagacgcag atcctgctcg gcgccatcgc cctgaagtcg cacgaagacc
     4921 gcgcctcgcc cgatgcggtg gaaggcatcg acccaacccc gtacaacgag gaggacggct
     4981 atctcggcgt ggtgcaccgc ttcggcgatt tcaccctgcg cctcggcggc gccatcgagc
     5041 acctcacctt cggcaacgtg ctcggcacgc atggcgagat caacaatgag gaccgcgacc
     5101 gcacccgcta caccttcggc gggttgctgc gctatgaggc caatccgaag ctgcggccct
     5161 tcgtcgaggt catgggcgat ctccgccgct atgtcacggt gccggacgat tttggtttca
     5221 accgcaactc cgatggcttc ctggccggcg tcggcacgct gtacacgctg accgacaagc
     5281 tcagcggcga ggtgttcctg ggcgtgctgc accggaacta tacagacccc agcttcaagt
     5341 cgctgaccac gccggcagcc gacgcaacct tgcgctggca ggcgaccggg cgcacggcgg
     5401 tggtgctgtt caccgagcgc acgatcgagg aaaccacgct gttcggcagt cccggctaca
     5461 tctacaccct ggtgggcgga cgcatcgaac agcgcctgac cgaccggctc accggcatcc
     5521 tgcgcgccgc ctatgcgcac agcgacttcg cccaggccgg ccgcaacgac aacgacgtcg
     5581 atacgtcggt gggtgtgcgc taccggctga ccagccgcgt cacgctgggc gtcgattatc
     5641 gctataccca gcgcgtgtcg ggcagcacgc tgaccgattt cagccgcaac caggtgttct
     5701 tccgcatcgg caccgagttc tgagcgccgg ccgccgcgcc acggcggccg ggtgccggcc
     5761 cggcgctaca ccaccgggct ggcgccgccg tccatgttga tcgccgcgcc ggtggtgaac
     5821 ccggccaggt cggagcacag gaagcaggcc atcgcggcga attcctccgc cttgccgatg
     5881 cggccgagcg gcacgttggc gcccatcttg gcgcggaatt cctcatagct ggcgttggag
     5941 ccctgttgct ggtgccgccg cacgtgctgg tcggaatcga tcaacccgac cagcagcccg
     6001 ttcaccagga tgccgtgcgg cgcgccctca ttcgccagca ccttcatgaa tgccatgccg
     6061 gcggcgcgcg acaccgcggt cggcgcgccg ttcgcccggg gcgccttggc gcccaggttc
     6121 agcacgctga tgatccgtcc ccatttgcgt tgctgcatgc ccggccacac cagccggacg
     6181 aagcggattg ccccgaacag cttcagctcc aggtcctcct gccagatctc gtcggtgatc
     6241 tgggtggagg gcatggcgcg cgaggtcccg gcgttgttca ccaggatgtc caccttgccg
     6301 aaggtcttga tcaccgcatc gaaggtgcgg gtgatctgat cggcctgcgc cacgtcgcag
     6361 gagaaggtct ccacccgccc ccgcgcgttc ttcgacgcga tggccttagc ttccgccagc
     6421 gcgtccggcc ggcgcgccag gatggcgaca tcggcgccgg acgcggcgaa ttcggtggcc
     6481 atggcaagcc ccagcccctt gctggcgccg gtgatgatgg cggtgcggcc gtcgagacga
     6541 acttccatgc gacttctccc cccacaggtt gccgccgagt gtgggcgagg cggcgttccg
     6601 cggcaaccgg cgatgccgtc cgcgccgcgc cgggcaacag ccggggtgcg attgcggcga
     6661 aagacagggt atgacgctgg cacgaccaca aggatgcgct ccggtgccga ccagccaacc
     6721 ccggctctgc ctgctcacgc ccgagtcaac gccgcccggc ccggattgcg cgggcctgtt
     6781 cctcgccttg cgcaagcacg ccaccacggc cggctatcgc gtgaccgaag cggcggtgag
     6841 cgaaaccggc atccgcattc tgggtccgga gcaagccgac ccggcgcccc tcccgcagcg
     6901 cttctttgga ccggccgcgg cacaggccgc ctatcgcgtg ctggaatggc tgcgcgccga
     6961 gccgttcgac atcgtctgcg ccccggccga ggcggggctg ctgttctacg ctctggccgc
     7021 ccgccaccag gccgtcgcac tcaccggcac cacgttctgc gtgctggccg agcggctgac
     7081 gctgctgtcg cgtcaccgcg ccggcgagat gccgcgcgac atggaagacc tggtacgcga
     7141 cgagatggag cgccggatgc tggcgctgcc cgatgccgtg ctggtgacgg attcgggcat
     7201 cgcgccctgg gtccgggagc agggctggac ggtcgccggt ctccgcgatt gcggcgatac
     7261 cccgcaggcc gcggtccagg cgctctggcc ggacctcctc gcggcggcgc gccgcgcggc
     7321 gccgccggcc gatgctgacg gggacatgcc gctggtctcc gtctgcatcc cgcatttcaa
     7381 ccgccccacc ctgctgtggc aggcggtcag ctcgatccgc gcccagacct acccaaggat
     7441 cgaactcgtc gtggtggacg atgccagcac cgacccggcc gcgctggcca cgctggacac
     7501 gctggaggcc gagctggcgg agcaaggcgg caagctggtc cggcaccccg tcaaccgcta
     7561 tctcggcgcc gcccgcaaca ccggagcccg gcacgccagc ggcgcgttcg tgctgttcct
     7621 ggacgatgac gactatgcca agccggaaca ggtcgccacg ctggtccgcg ccgcccaggc
     7681 caccggcgcc gacatcgtca actcgctctg cgagcggctg cagggccagg agccgcccaa
     7741 gccggggcag caatccgaag agcgctggct gaccctcggc aatgtcccga ccctcggcct
     7801 gttccgcaac ctgttcggca ccgcctccgc cctgttccgc aagaccagct tcgagcggct
     7861 cggcggcttc accgagatgc acggcatcgg ctgcgaggat tgggagatct tcgcccgggc
     7921 gtcgctcgcc gggatgaacc tgcagacggt gccggaggcg ctgtactggt accggctgac
     7981 gcagggcagc atgatccaga ccggcaatgt gtatgccggc gagagtcggg cgatggcgcc
     8041 ctacctggcc ctggtgccgc aggtgctgtg gccggcgttg cgcttcgcgc acggcgcgga
     8101 gcagcggttg cagactgcac aggacagcct gcgccatggc gaggccgtgc tgcgccatcg
     8161 cgacgcgctg atcgcccatc gcgagcggca catcgcgcat ctgacgcaat tggaccgcga
     8221 acgcgccgaa ccgctggccg ccctgtcgcg ggaggtcgcc gagctgcgcg cggtgctgcg
     8281 cgtcacgatg ggcggctatg gaccgacgcc gcaatcggcg gtggattcga tgctgcaatc
     8341 cggctcctgg cgactcgggc gcctgctgca ggcgccgctg cgctggatgc gcgggcgccc
     8401 ggagaccgcc ccgccgcggg tgacggactg ggtgcaagcg gccagcgtga tcaacagcat
     8461 gcaacgctcc accacctggg agatgaccgg gctgctgcgc gcgctggccg gcctgctgcg
     8521 gcgccgctga gccgcatctt gcgcggcccc gccgccggcc cgatcttttc ccgcaaggag
     8581 aagccaatgc ccgaaatcga gatctacacc cagccctggt gtccctattg cgcgcgcgcc
     8641 ttgcacatcc tgggcgaaaa gggcgtcgcg ttccgcgaaa tccacgcacc gaacggcacg
     8701 ccggaacgcg aggaggcacg caaccgctcc ggcggacgca ccagcgtgcc gcagatcttc
     8761 atcgacggcc ggcatgtcgg cggctgcgac gacctggtgg cgctggaccg ggcgggcaag
     8821 ctggatccgt tgctgcatcc ggcgtgaaac gccagcgtca caaatccgct ggcagtccct
     8881 tggcgggagt gccaaactcg ccatctgaga cacgacgcac gcggcaaacg caggacgggg
     8941 tatgatccac taccagttgc gctgcagcca gcagcacgag ttcgacggct ggttcaagga
     9001 cagcgcggca ttcgaacagc aggccaagcg cgggctggtt gattgcccgg tctgcggcga
     9061 tgtgcaggtg acccgcgcgc tgatggcgcc cgcggtgacc acgcgcgagg ccgcgccgcc
     9121 gcagccgccg caacccgcgc cgcaatcgcc ggccgctccg gcggaggcac cgccggcgcg
     9181 catggccggc ggcaagctgc cggcgcacat ggtggcgatg ctgcagcgcc tgcgctcgga
     9241 ggtggagaag aactgcgatt atgtcgggcc ggccttcgcc gacgaggcgc gcaagatcca
     9301 tcgcggcgag agcgagaagc gcggcatcta tggcgaaacc acccccgacc aggcagaatc
     9361 gctggccgag gaaggcatcg aggtagcccg catcccctgg gtcccccgcg ccgacgggta
     9421 gcggcgggcg cagaaacaat cgatccgcgt ccacgagtcg accggaacgc gatccccccc
     9481 gtgtcatgcc cgcgcttggc gcgggcaccc acgtcttgtc agcctgcaca gagtagaaga
     9541 tgtgggggcg ggcctccgcc ggccatgaca cgaatcgcct gcaacgcgga ctggcagaat
     9601 tccccgctca gccttccgcg gcgaccaggt agttcaccga caggtcgcgc accgcccgcc
     9661 agccgccgcg cagcccatcc ggctgcagcc cgctgatatc cgccgtgcgc agcccggcgc
     9721 cgtgcagcag tgacgccagc tccgccgggg tgatgaactt gcgccagtca tgcgtgccca
     9781 ccggcagcca gcgcagcagg tattcggcgc cgaccttggc ggcgaggaag gattgcggcg
     9841 tgcggttcag cgtggacagg aacagcagcc cgccgggcgc cagcaacccg gcgagcacgt
     9901 gcaggaaagc cgccggatcg ggcacgtgct cgatcacctc cagcgcggtg attgccggaa
     9961 agcgcgcgcc ctcggccagc aggtcctccg cggtgccgct gcgataggcc agcgtgaggt
    10021 gctgcccggc ggcatgcgcc tgcgccgcct cgatcgcctc gccggcggca tccagcccga
    10081 gcacgtcgaa gccctggcgg gccagcgctt cggaggccag cccggcgccg cagcccaggt
    10141 cgagcagccg cagcccctgg tgctgcggga atcggcgggc gatgcgctcg cagatccagc
    10201 cgatgcgtgc cgggttcatc cggtgcagcg ggcgcatcgg cccgtccggg tcccacctgc
    10261 gcgccgcgag cgcgtcgaac cgtgccactt cgtccgctga aacggtcgcg ccgctcatgc
    10321 cgagcctccg ggatttgctg ctatgtgcgg ccggcggcgg cggctggcaa cgccggaggg
    10381 atcacgctcg gcgccacgtg ggcggcatgg cgcgcaacag gtaggctgtg ggcgcgctgc
    10441 gccaagtgat gtttgtctag cgaaaggggc aatcgcgggc cgaaggaggt caagatcgtt
    10501 gtgggatcgc gcccgaactt ccgatcagac cgtcgcccat gagtggattc cattcgatgc
    10561 gcgacttgcg gttgtccgca gcgctggcac aatcggtggc gaccgctccc tttgctgatc
    10621 ctcggttccg cgagctatcc cggctgctta atgagagtcg cgactgaaac gtgtggccag
    10681 ctcaaattat taaaattgag gagcacggtc tgggtgtact gaagctggtt aacgatgcgg
    10741 ccgtcgggct cctcaatcgt ttcaagccag aagattgcct ccatctgggc ggccgaggca
    10801 ttgggtccac cagcggcacc ctgcaagaag gcaatattcg agatgcctcc gcccgaggaa
    10861 ggcggtttca ggggttgagt cgaaattttt agcgtcatcg ttgaaatgac gtttttgccc
    10921 tgcagtccca acttcaaaaa cttattggga ttgtccacga tctcctgggt aacccgaggg
    10981 atgtcctccg gatcggttcg gaattctgtc cgggttgcaa ggttgctttc gggaaattga
    11041 accggactgt gcggcggtgc aatcgtgaag ggggtgatgc tcacggggtc aatatgcggg
    11101 gcttcgttga tgggaggcaa cgccgttccc tgggcgatta gcgacgttcc gtgaggaatg
    11161 ttggcgaggc gggcgacagt tgttggatcc tgcgggttcg acgtcatcgg cacatgaatc
    11221 cagatccctg gctccacgtg gataccggcc ggctcaccat tcggaccgag gacattggcg
    11281 tcctggactt gttgaagata ggtcaagccg tgcagagtga tgtccggttg gaggaagcct
    11341 cggttgggaa tgtcgccggg aa
//
LOCUS       DASSZH010000369         2153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1406246, whole genome shotgun sequence.
ACCESSION   DASSZH010000369 DASSZH010000000
VERSION     DASSZH010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406246"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="VFN42_08085"
     CDS             complement(<1..1051)
                     /locus_tag="VFN42_08085"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8996612.1"
                     /translation="MCTYCDRGFPDLHGIRFDRRSLLKTAAAGVVTIGLPFAKTANAA
                     SPITLKGTHGTGFCNSAFFITHMRHLAKKDGVTLQFVNTPTNADAVTFFATGQVDVSV
                     LPYTSFLALHGAGAPVKIVAGGGIQGCTIIAQPGLDTPAKLKGKTLGTFQLDTLEVLP
                     YDWLKKHGLSFKDINVRYLGSTPEQVEAFKAGAIDIAGVIEPYASALLNDVKGAVLLS
                     DGIDIYGPQYTDCVLAARTELMEKDPDAVVALIKAMIHGQYLFESEREKMLKELVGPY
                     YKLSLENARIGATKQPPKVDQRAETDFILTRVQSVIEMGYLKKPPGKDAIDWKLLEVA
                     IKESSDVYPKLKYKSA"
     gene            1309..2091
                     /locus_tag="VFN42_08090"
     CDS             1309..2091
                     /locus_tag="VFN42_08090"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET8996613.1"
                     /translation="MSQGSVRDSVAASRANRPGNARARPASGMRAHSGFWAEVQNDEL
                     VSAVTDAIAAGRLTAGTKLGEEELGALFGLGRTRVRQALRTLAFAGLVQLEPNRGAFV
                     ACPSLEEAEAVYAARRLIEGEIVREATRHCTANDIRRLRAHCAAQAAAQAEQPAYLRL
                     LSEFHLLIAEIGGNMVLAELLSGLVPRTALMQALYQPPGEGGGPCAVDHHLHIIQLMA
                     AGDTEGAAAAMAAHLTINLRALDVGAGPPAADLAAALGVVRA"
BASE COUNT          367 a          667 c          740 g          379 t
ORIGIN      
        1 aagccgattt atacttcagc ttcgggtaga catcggacga ttccttgatc gccacctcca
       61 gcagcttcca gtcgatggcg tccttgcccg gcggcttctt caaatatccc atttcgatca
      121 ccgactgcac gcgggtcagg atgaagtcgg tctcggcgcg ctggtccact ttcggcggct
      181 gcttggtggc accgatgcgg gcattctcca gcgacagctt gtagtacggg ccgaccagct
      241 ccttcagcat cttctcacgc tcgctctcga acagatactg gccatggatc atggccttga
      301 tcagcgcgac gacggcgtcg ggatccttct ccatcaactc ggtgcgcgcg gcgagcacgc
      361 aatcggtgta ttgcgggccg tagatgtcga tgccatcgga cagcaacacc gcgcctttga
      421 cgtcgttgag cagcgcggag gcgtacggct cgatgacacc ggcgatgtcg atggcgccgg
      481 ccttgaacgc ctccacctgt tccggggtgg agccaagata gcgcacgttg atatccttga
      541 acgacaggcc atgcttcttc agccagtcgt acggcagcac ttccagcgta tcgagctgga
      601 aggtgcccag cgtcttgccc ttcagcttgg ccggcgtgtc cagtccaggc tgggcgatga
      661 tggtgcagcc ctgaatgccg ccgccggcga cgattttgac cggcgcgccg gcgccgtgca
      721 gcgccaggaa gctggtgtag ggcagcacgc tgacatccac ctggccggtg gcgaagaagg
      781 tgaccgcatc ggcattggtc ggcgtgttga cgaattgcag cgtgacgccg tccttcttcg
      841 ccagatgccg catgtgggtg atgaagaagg cgctgttgca gaagccggta ccgtgggttc
      901 ccttcagggt gatcgggctg gcagcatttg cggtcttggc gaagggaagg ccaatggtca
      961 cgaccccggc cgccgcggtt ttgagcaggg atcgacggtc gaagcggatt ccatgcagat
     1021 cgggaaagcc gcgatcgcaa taggtgcaca tggctaggga ctccctgggg tggcgacatt
     1081 gctgcgcagg ccgggtgcgc gattaccgtg cagcaaggat cgtgccagcg gaaaatgcca
     1141 agtaagagcc cctttatcga gcggtatgct tatttttacg tcttagtcct gcctcctggg
     1201 cgtcacgcag ggcagattgt acgcaatatc actgattgag gacaattcga cccgccagcg
     1261 ctgggcagcg gacggcaagc ctgctatcga ccggcccgag gtgaggcgat gagtcaaggc
     1321 agcgtgcggg acagcgtcgc ggcgagccga gcgaacagac cgggcaatgc gcgggctcga
     1381 ccggcatccg gcatgcgggc gcatagcgga ttctgggcgg aagtgcagaa cgacgagctg
     1441 gtcagcgcgg tgaccgatgc catcgccgcg ggccgcctga ccgccggcac caagctcggt
     1501 gaggaggaac tcggcgccct gttcggcctc gggcgcaccc gggttcgcca ggcgctgcgg
     1561 acgctggcat tcgccggcct ggtgcagctg gaaccgaacc gcggcgcctt cgtcgcctgc
     1621 ccctcgctgg aggaggccga agcggtctat gcggcacgcc ggctgatcga aggcgagatc
     1681 gtgcgcgagg caacccgcca ctgcaccgcc aacgacatcc gccggctgcg cgcgcattgc
     1741 gcggcgcagg ccgcggcgca ggcggaacag ccggcctatc tgcggctgct gagcgagttc
     1801 cacctgctga tcgccgaaat cggcggcaac atggtgctgg cggaattgtt gagcgggctg
     1861 gtgccacgca ccgcgctgat gcaggcgctg tatcaaccgc ccggcgaggg cggcggccca
     1921 tgcgcggtgg atcaccacct gcacatcatc cagctgatgg ccgcaggtga caccgaaggc
     1981 gccgcggcgg cgatggcggc acatctgacc atcaatctgc gcgcgctgga tgtcggcgcc
     2041 gggccgcccg cggccgatct cgcggcggcg ctgggagttg tgcgggcgta aggccgctgg
     2101 cggcgcgtac cgcgtcgtgg cgggcgaagg ttcaccacga cgtgcgttgc ttc
//
LOCUS       DASSZH010000370         3199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_554287, whole genome shotgun sequence.
ACCESSION   DASSZH010000370 DASSZH010000000
VERSION     DASSZH010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554287"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VFN42_08095"
     CDS             complement(<1..389)
                     /locus_tag="VFN42_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014626163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HET8996614.1"
                     /translation="MTGNDPAQTYRQEAAELLEQLEQALLDLEHMPGDADLVNTAFRA
                     LHTIKGSGAMFGFDRVAQFTHHVETAFDRVRKGQTAVSPPLIAVALAAKDQIRCLIEQ
                     PEHADDAAGAAVLLQLQRALEAPHAEA"
     gene            complement(386..754)
                     /locus_tag="VFN42_08100"
     CDS             complement(386..754)
                     /locus_tag="VFN42_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014626162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8996615.1"
                     /translation="MARTILSVDDSASVLQMVKLTLTGGGYQVMQAGNGAEGLAKAKA
                     GGIDMVLTDLNMPVMDGIAMIRELRKLPHCTGVPIIFLTTESAANMKQEAKAAGATGW
                     ITKPFQQDQLLAVVRKVLGT"
     gene            complement(754..1068)
                     /locus_tag="VFN42_08105"
     CDS             complement(754..1068)
                     /locus_tag="VFN42_08105"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET8996616.1"
                     /translation="MSDQPDSIPRIGLHGAMSIRDAGALHTDLVHALSLHTTVHIDGS
                     DLESADLSVIQVLLAAHRSARASGKQVLLHPPPAAALAEVLQRGGFVQAGANCDAFWS
                     GR"
     gene            1229..2080
                     /locus_tag="VFN42_08110"
     CDS             1229..2080
                     /locus_tag="VFN42_08110"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET8996617.1"
                     /translation="MKALTQEQVESYRRDGFLFPVAGLNATEIAACLAGLERLETALG
                     CAVADADIKWRSHAYAHSPWFDDLIRHPRILDAVEDVIGPDILVWTSTFFIKEPNSPT
                     FAAWHQDGAYFGLQPKEQVCAWVALTDASREAGCMEQLSSRGQPRLRHHAALGMAHSI
                     NRAGQTIMEPFDESAPVAMALRAGEFSLHHELAVHRSAPNNASHRRVGIGLNFIPTHV
                     RVDGPVRPMAMLVRGEDRYGHFDLFDPPRAERDAAALALHQRVSERYRENYRYQVERH
                     AQDFAAA"
     gene            complement(2237..>3199)
                     /locus_tag="VFN42_08115"
     CDS             complement(2237..>3199)
                     /locus_tag="VFN42_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HET8996618.1"
                     /translation="TRFDIEVMHAEGSWVGAGEPIVYVSGSLLALSDLETIMLQKIGP
                     ACVAAHNAFQMCLALPRAAFLAMEARHCAGAEMQEMMAYAASVGSSAAKQEGARGFIG
                     NANDGTAHWFGASRGSGTMPHSLIGYAGSTLRAAEMFRETFPDEPMTVLVDYFAREIT
                     DALAVCARFADLAASGGLSFRLDTHGGRFVEGLDPAESYAVLERHAPGAIRRYRSETE
                     LRYLVGTGVSAAAIWRFREALDSAGYPKVRIVVSSGFGVDKCRVMADAKAPIDVVGTG
                     SFIPDSWHETYATADIVEYDGVPMVKVGREFLLRRNGARRKAGG"
BASE COUNT          539 a         1067 c         1048 g          545 t
ORIGIN      
        1 tctgcctccg catgtggtgc ctccagtgcg cgctgcagct gaagcaggac ggcagcgccg
       61 gcggcatcat cggcgtgctc cggttgctcg atcaggcagc ggatctggtc ttttgccgcc
      121 agggccaccg cgatcaacgg cgggctgacc gctgtctggc ctttgcgcac gcgatcgaat
      181 gcggtctcca cgtgatgcgt gaactgggcg acgcggtcga agccgaacat cgcaccagag
      241 cctttgatcg tgtgcagcgc acggaacgcg gtgttgacca gatcagcgtc gccgggcatg
      301 tgttccaggt ccagcagggc ctgttccagt tgttccagaa gctccgctgc ttcctgacga
      361 taggtctggg cgggatcgtt gccggtcatg tgcccagcac cttacggacg acggcaagca
      421 actggtcctg ctgaaagggt ttggtgatcc agccggtagc gccagcagcc ttggcttcct
      481 gcttcatgtt cgccgccgat tcggtggtga ggaagatgat cggcacgccg gtgcaatgcg
      541 gcagcttgcg cagctcgcgg atcatggcga tgccgtccat caccggcatg ttcaggtcgg
      601 tcagcaccat gtcgataccg ccggctttcg ccttcgccag gccttccgcg ccgttgccgg
      661 cctgcatcac ctggtagccg ccgccggtga gcgtcagctt caccatctgc agcacgctgg
      721 cggagtcatc gacgctgagg atcgtcctgg ccatcaccgg cctgaccaga atgcgtcgca
      781 gttcgcgccg gcctgcacga atccgccgcg ctgcagcacc tcggcgagcg cggcagctgg
      841 cggcggatgc agcaggactt gcttgcccga tgcacgcgcg ctgcgatgtg ccgcgagcaa
      901 cacctgtatg acgctgagat ccgcagattc gagatcgctg ccgtcgatgt gtaccgtcgt
      961 atgcagcgac agggcgtgca caaggtcggt gtgcaatgcg ccggcgtcgc ggatgctcat
     1021 ggccccatgc aagccgatcc gcgggatcga gtcgggctgg tcggacaacc gatcctccta
     1081 tgcgctgctg acctcccgga tttctggcat gcgaggatta acaaagcgtg tgcggccaaa
     1141 agattccgcg gcggatgttt ggggctggca ggtttgcgct acactggcct ttggccgccg
     1201 ggcgcggccc aatcacagga ggagaccgat gaaggctctc acgcaggagc aggtagaatc
     1261 ctatcgccgc gacggctttc tgtttcccgt tgcgggcttg aacgcgaccg agatcgccgc
     1321 ctgcctggcc ggactggagc ggctggagac ggcactgggc tgcgccgtcg cggacgccga
     1381 tatcaagtgg cgctcgcacg cctatgcgca ttcgccctgg ttcgacgacc tgatccgcca
     1441 tccccgcatc ctggacgcgg tggaggacgt gatcggtccg gacatcctgg tgtggaccag
     1501 cacgttcttc atcaaggaac ccaactcccc gacttttgcc gcctggcacc aggacggcgc
     1561 gtatttcggc ctgcagccga aggagcaggt ctgcgcctgg gtggcgctga ccgatgccag
     1621 ccgcgaggca gggtgcatgg agcaactgtc ctcgcgtggg cagcctcggc tgcggcatca
     1681 tgccgcgctg ggcatggcgc acagcatcaa ccgcgcgggc cagacgatca tggagccgtt
     1741 cgatgaatca gcgccggtgg cgatggcgct gcgcgccggt gaattctcgc tgcatcacga
     1801 gctggcggta catcgctccg cacccaacaa tgcgtcgcat cggcgggtgg gcatcgggct
     1861 gaacttcata ccgacacatg tgcgggtgga cggcccggtg cggccgatgg cgatgctggt
     1921 gcgcggggag gaccgctacg ggcatttcga cctgttcgat cctccgcgcg ccgaacgtga
     1981 tgccgccgcc ctggcgctgc accagcgggt cagcgaacgc taccgcgaga actaccgcta
     2041 tcaggtcgag cggcacgcgc aggacttcgc ggccgcgtaa cagcaacccg cgataagcat
     2101 gccgcccccg tcatgccgaa aggccggcat ccacgacttt cctcagttct acacagcaaa
     2161 gtcgtaggtg gcgggtctgc aaccgccatg acgcggtact gccgccggtg cgtcattcac
     2221 ccggcgggct ggtatactat ccgcccgctt tgcgccgcgc accattgcgg cggagcagga
     2281 attcgcgacc gaccttcacc atcggcacgc cgtcatattc gacgatatcc gcggtcgcat
     2341 aagtctcgtg ccaggaatcc ggaatgaagc tgccggtgcc gaccacgtcg atcggcgcct
     2401 tggcgtcggc catcacccgg cacttgtcca cgccgaagcc ggaggacacc acgatgcgca
     2461 ccttgggata gcctgcggaa tccagcgcct cgcgaaaacg ccagatcgcg gcggcggaca
     2521 cgccggtgcc gaccaggtag cgcagctccg tttcgctgcg gtagcgccgg atcgcgcccg
     2581 gggcatggcg ttccagcacg gcatagctct cggccggatc caggccttcc acgaagcgcc
     2641 cgccgtgggt atccagccgg aacgacagcc cgccggacgc agccagatcg gcgaaccgcg
     2701 cgcacacggc cagcgcatcg gtgatctcac gcgcaaagta atccaccagc acggtcatcg
     2761 gctcgtcggg gaaggtttcg cggaacatct ccgccgcgcg cagggtcgat cccgcatagc
     2821 cgatcaggga gtgcggcatg gtgccggacc cgcgcgacgc cccgaaccag tgcgcggtgc
     2881 cgtcattggc attgccgatg aagccgcgcg caccttcctg cttcgccgcg gaactgccga
     2941 ccgaagcggc gtaggccatc atctcctgca tctccgcccc ggcgcagtgg cgcgcctcca
     3001 tcgccaggaa cgccgcgcgc ggcagggcga ggcacatctg gaaggcgttg tgcgccgcca
     3061 cgcaggcggg accgatcttc tgcagcatga tcgtttcaag atccgagagc gcgagcaggc
     3121 tgccggagac atagacgatc ggctcgccgg cgccgaccca gctaccttcc gcgtgcatca
     3181 cctcgatgtc gaagcgcgt
//
LOCUS       DASSZH010000371         5427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_980440, whole genome shotgun sequence.
ACCESSION   DASSZH010000371 DASSZH010000000
VERSION     DASSZH010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980440"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /locus_tag="VFN42_08120"
     CDS             <1..1363
                     /locus_tag="VFN42_08120"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HET8996619.1"
                     /translation="RRARRPMRLVGASGQLGYGVPTPAFEAALARQPDLIGADMGSID
                     IGPTYLGKGEMATSPAATRRDLRKALLGARSIDVPLVIGSAGSAGAAPHLEATLAIVR
                     EIARADGLHFRLGVIRADLPRPMLLEAVRAGKVRGIDDMPALTEEDVTEAAQLVGQMG
                     MGPFRRALAEDIDVLIAGRACDTAIFASLPTMLGYPTGLSVHMAKIIECASLCCLPGG
                     RDCILAELDDEGFVLESMNPQRRATPVSVAAHSLYEQADPFTVYEPEGRLDLSTAHYA
                     AVDDRRTRVTGARWAPSPDPCVKMEGARKIGERAVLLSGAADPRFIAQHRELLAAVSD
                     VVRDLVCEDTPQDYTLRFRVYGVDGVRMAAPPGEPPPGEVFVMGECIAPTGERAAEVV
                     RTCKQYLLHHGYAGRLSTAGNLAFPFTPPEVSLGPAYRFNVYHLLHTPDPDALFPLEI
                     ETL"
     gene            complement(1733..2719)
                     /locus_tag="VFN42_08125"
     CDS             complement(1733..2719)
                     /locus_tag="VFN42_08125"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HET8996620.1"
                     /translation="MILLTNNEGTVGVPTTARRLAERAPALDAIEAGIRLIEADPSVR
                     TVGRGGWPNLLCQMELDACVMDGDTLRSGAVGALQGFLHPVSVAREVMRQLPHELLAG
                     EGAARFAREIGAEPAENLIEDSRRAWQAWFDAELSGAQKRDWPEIPLAALCRHAIDPE
                     TVGEAGQDTTVFLAQDAHGTIASGTSTSGWGWKYPGRLGDSPIIGAGSYADTRYGAAA
                     CTGAGEMTIRCGTARSIVLYMKRGASVTDAVMEAVDDMRALRGGLISRVTIHAIDTRG
                     NHKVVAVNGTGENTYWLWEGTGAPQSLRAEPVAIGEVDAKPTASVRYGYGRS"
     gene            2791..3645
                     /locus_tag="VFN42_08130"
     CDS             2791..3645
                     /locus_tag="VFN42_08130"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8996621.1"
                     /translation="MRPALALLLLLFGFSARSVVAATVHDRYFSTSDGVRLHYLEAGP
                     TRAHTIVFVPGWTMPAWIWTPQIIAFARRYHVIAFDPRGQGNSAAPDFGYTPARRGDD
                     IADLLSHTGGAPVLIVGWSLGVLDTLAYVHRHGDRRIAGLVLVDNSVGEEPPPHPTHP
                     HPGPKLPRPAMMRHFVQGMFRHAPGERYLERLTAATLHTPEAASRALLSYPEPRSYWR
                     DAVYSTSKPVLYVVRPIWFAQADNLKRKRPNTEIARFRDAGHALFVDDAGRFNTLLSS
                     FITRRIWP"
     gene            3642..>5427
                     /locus_tag="VFN42_08135"
     CDS             3642..>5427
                     /locus_tag="VFN42_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996622.1"
                     /translation="MKLLPLFLVSLSAVGYETALTRYFAVSSWSEYGYWVISIVMVGF
                     ALSGVVLALHRDGLARHGARLLPALPPLLVAAGALGFHLVTLNPFNPLQLQNSATWQD
                     QLWNIGGYYLDLLPFFFLAGLFVSLSFVLNAREIGRVYGFDLTGAAAGSALVLAAMYV
                     AHAFYLVPLLLVPLAAAALFIPGRRRVATVGATLAVLLAAEALLLLDNQAAFNQFKPV
                     YAPLHTPNARVVARVLSPRGDYLLLNDFTERVDTDISNNTGLLHVSGPPRSFGLYRDG
                     NRIAALPRSGTPDAGYAAAALDAAPYLLRPHASVLLAGADGGFRIAELLALGAARIRV
                     LEPEPTLFRALRHGLGPSPAWPADGRVTLSDLGPIAAADRGARYDIIDLSGDFLDAAE
                     ANASAFTVQAMVRYLAALSPGGMVSLPVNIRDFPVYALRLLATARQALRLAGVHDPAA
                     HVMLYRSAWDARLLLSGTPWTASQIAALRKFCDDRSFDVSWYPGIDIGKARAGIYNDL
                     PAVSFASGEMAASGPDDSIADEAGAILAGQPSPSRDAFSLAPITLDRPFFYAVLRLDQ
                     LGTILQRLEILPQQEIGALVNLAVLAQAA"
BASE COUNT          772 a         1973 c         1750 g          932 t
ORIGIN      
        1 ccgccgtgcc cgtcggccta tgcggttggt cggcgcttcc ggccagcttg gctatggcgt
       61 gccgaccccc gctttcgagg cggcgctggc ccggcaaccg gacctgatcg gcgccgacat
      121 gggctcgatc gacatcggcc cgacctatct gggcaagggc gagatggcca cgtcacccgc
      181 ggcgacgcgg cgcgacctgc gcaaggcgct actcggcgct cgctcgatcg acgtgccgct
      241 ggtgatcggc tcggccggat cggccggcgc cgccccgcac ctggaggcca cactcgccat
      301 cgtgcgggag atcgcgcgag ccgacgggct gcatttccgc ctcggcgtca tccgcgccga
      361 cctgccgcgc ccgatgctgc tggaggcagt gcgcgccggg aaggtccgcg gcatcgacga
      421 catgcccgca ttgaccgagg aggacgtcac cgaggccgcg caactcgtcg gccagatggg
      481 catgggcccg ttccggcgcg ccctggccga ggacatcgac gtgctgatcg ccggacgcgc
      541 ctgcgacacc gcgatcttcg cctcgctgcc caccatgctg ggctatccca ccggcctttc
      601 cgtgcacatg gcgaagatca tcgagtgcgc ttcgctgtgc tgcctccccg gcggccggga
      661 ctgcatcctg gcggagctgg atgacgaggg cttcgtgctg gagagcatga acccgcagcg
      721 cagggccacc cccgtctcgg tggccgcaca ttcgctgtac gagcaggccg acccgttcac
      781 cgtttatgaa ccggagggca ggctcgacct gagcaccgcg cactatgccg ccgtggacga
      841 ccggcgcacc cgtgtgacag gcgcacgctg ggcgccctcc cccgatccct gcgtgaaaat
      901 ggaaggcgcc cgcaagatcg gtgagcgcgc cgtgctgctg tccggcgccg ccgatccccg
      961 cttcattgcc cagcaccggg agcttctggc agcagtgtcg gacgtggtgc gcgacctcgt
     1021 ctgtgaggac acgccgcagg attataccct gcgcttccgc gtctacgggg tggacggagt
     1081 tcggatggca gctcccccgg gagagccgcc gccaggcgag gtgttcgtga tgggcgaatg
     1141 catcgccccg accggggagc gagctgccga agtggtgcgg acctgcaagc agtacctgtt
     1201 gcaccacggt tatgccggcc gtctttccac cgccggcaac ctggccttcc ccttcacccc
     1261 accggaagtc tcactcggcc cggcctaccg cttcaatgtg taccacctgc tgcacacgcc
     1321 cgatccagac gcgctattcc cgctggagat cgagacactg tagaaccttg cgcccccagg
     1381 cagccgctcg cggcacccaa ggtcacgccg cggaagccca gcgccgcggc gctgggtgat
     1441 gcctggccgc atcggccgcc gcaacacgag cctcgtattc gaattccgcg gcaacctctc
     1501 ggagagcgcg gaccaccatc ggatcccgcg ccgtttcggc agcactgcgg agcgccgcag
     1561 cacgtgcccg caggtaagcg gtctcgttca ttgcacccat gccttgttgg tgaaccccat
     1621 ggcaggttca acgggaagcc gcggcacgag tttctcgcca tggtaccggg acctttgcag
     1681 gatcccgtgg tctgcaatcg gaattccccc ggctgcgcgc ggtcgcggcc agctagctcc
     1741 ggccgtagcc gtagcgcacg ctcgccgtcg gcttcgcatc cacctccccg atcgccaccg
     1801 gctcggcacg cagcgattgc ggtgcgcccg tcccttccca aagccaatag gtgttctcgc
     1861 cggtcccatt caccgccacc accttgtgat tgccccgggt gtcgatcgca tggatcgtca
     1921 cgcggctgat cagcccgccg cgcagcgcgc gcatgtcgtc cacagcttcc atcaccgcgt
     1981 cggtcacgct ggcaccgcgc ttcatataca gcacgatgga gcgggccgtg ccgcagcgga
     2041 tcgtcatttc gcccgccccg gtgcaggcgg cggccccgta ccgcgtgtcc gcataggacc
     2101 ccgctccgat gatggggctg tcgccgagcc gaccgggata tttccagccc caaccggagg
     2161 tgctggtgcc gctggcgatc gtgccgtggg cgtcctgcgc caggaacacc gtggtgtcct
     2221 gcccagcctc cccgactgtt tccggatcga tggcgtggcg gcacagcgcg gccagcggaa
     2281 tctccggcca gtcgcgcttc tgcgcgccgg agagttccgc gtcgaaccag gcctgccagg
     2341 cacggcggga atcctcgatc aggttttccg caggttccgc gccgatctcc cgagcaaagc
     2401 gggcagcgcc ttcacccgcc aggagctcgt ggggcaactg gcgcatgacc tcacgggcga
     2461 cgctcaccgg atgcaggaag ccctgcaaag caccgaccgc accgcttcgc agcgtgtcgc
     2521 catccatcac gcaggcatcg agctccatct ggcacagcag attcggccag ccgccgcggc
     2581 caaccgtgcg caccgagggg tcggcctcga tcaaccgaat gcctgcctcg atggcatcca
     2641 gcgccggggc tcgctccgcc agcctgcgcg cggtggtcgg cacaccgacg gtgccttcgt
     2701 tgttggtcag caggatcatg ggggggctcc tctgggcgac ggagaacaag gcgattgaca
     2761 cgacacggaa cgccccggtt ctctcccggt atgcggcccg cactggcttt gctgcttctg
     2821 ctgttcggat tttccgcccg cagcgtcgtg gcggcaaccg tgcacgaccg gtatttttcg
     2881 acctccgacg gtgtgcggct gcactacctg gaagccggac cgacccgggc acacaccatc
     2941 gtcttcgtgc ccggctggac gatgcccgcc tggatctgga cgccgcaaat cattgccttc
     3001 gcccgccgct atcacgtcat agccttcgac ccgcgcggcc agggcaattc ggccgcgcct
     3061 gatttcggct ataccccggc ccggcgcggc gacgatatcg cggacctgct cagccacacc
     3121 ggaggcgcac cggtcctgat cgtcggttgg tcgctcggcg tgctcgacac gctggcctac
     3181 gtgcaccgcc atggcgacag gcggatcgcc gggctggtgc tggtggacaa ctcggtcggc
     3241 gaggaaccgc cgccgcaccc cacccacccg catcccggcc cgaagctgcc ccgtccggcg
     3301 atgatgcgcc atttcgtgca gggcatgttc cgccacgcac cgggcgagcg ctacctggaa
     3361 cgcctcaccg ccgccacgct gcatacgcct gaagccgcca gccgcgcact gctgtcctac
     3421 cccgaaccgc gcagctactg gcgcgacgcg gtgtattcca ccagcaaacc cgtgctgtac
     3481 gtggtgcgcc cgatctggtt cgcccaggcc gacaatctga agcgcaagcg tccgaatacc
     3541 gagatcgccc gcttccgcga tgccggccac gcgctgttcg tcgatgacgc cggccgtttc
     3601 aacacgctat tgagcagctt catcacgcgc cggatctggc cttgaagctg ttgccgctat
     3661 tcctggtctc gttgtccgcg gttggctacg agaccgctct aacccgctac ttcgccgtca
     3721 gcagctggtc ggaatacggc tactgggtga tctccatcgt catggtgggg ttcgcgctga
     3781 gcggcgtggt gctggcgctg catcgtgacg gcctggcccg gcacggcgcg cggctgctgc
     3841 cggccctgcc gccgctgctg gtggcggccg gcgcgctcgg gtttcacctg gtcacgctca
     3901 atccgttcaa tccgctgcag ttgcagaatt ccgcaacctg gcaggaccag ctgtggaata
     3961 tcggcggcta ctatctggac ctgctgccgt tcttcttcct tgccgggctg tttgtcagcc
     4021 tgagcttcgt gctgaacgcc cgcgagatag ggcgcgtgta cggcttcgac ctgactggcg
     4081 ccgccgccgg ttcggcactg gtgctggccg cgatgtacgt cgcgcacgcc ttctatctcg
     4141 taccgctgtt gcttgtgccg ctcgccgccg ctgcgctgtt catccccggg cgccggcgcg
     4201 tggccacggt tggtgcgact ctggcggtgc tgctcgcggc cgaagcgctg ctgctgctgg
     4261 acaatcaggc ggcgttcaac cagttcaaac cggtctatgc gccgctgcac acgccgaacg
     4321 cccgggtggt cgcgcgcgtg ctttcgccgc gcggcgacta tctgctgctg aacgacttca
     4381 ccgagcgcgt cgacaccgac atttccaaca acaccggcct gctgcacgtc tccggtccgc
     4441 cgcgcagctt tggcctgtat cgcgacggca atcgcatcgc ggccctgccc cggtccggca
     4501 cgccggacgc cgggtatgcc gcggcggcac tggatgccgc accctatcta ttgcggccgc
     4561 atgccagcgt gctgcttgcc ggcgccgacg gcgggttccg catcgccgaa ctgctggccc
     4621 tgggcgccgc cagaattcgc gtgctcgagc cagaacccac attgtttcgt gccttgcggc
     4681 acgggctggg cccctcgccc gcctggccgg cagacgggcg cgtgacctta tccgatctcg
     4741 gaccgatcgc cgccgcggat cgcggcgcac gctacgacat catcgatctg tcgggtgact
     4801 tcctggatgc ggcagaggcc aacgccagcg ccttcaccgt gcaggcaatg gtccggtacc
     4861 tggcggcgct gagtccgggc ggcatggttt ccctgccggt gaacatccgc gacttcccgg
     4921 tctatgcgct gcgactcctg gccaccgcgc ggcaggcgct gcggcttgcc ggcgtgcatg
     4981 atccggcggc gcatgtgatg ctttaccgct cggcctggga tgcccggctg ctgctgtccg
     5041 gtacgccctg gaccgcgtcc cagatcgccg cactgcgcaa gttctgcgac gaccggtcct
     5101 tcgacgtctc ctggtatcca gggatcgata tcggcaaggc gcgtgccggc atctacaacg
     5161 acctgccggc ggtatccttc gccagcggcg agatggcggc gagcggcccg gacgattcga
     5221 tcgccgacga ggcgggggcg atcctggccg gacagcctag cccgtcgcgc gacgccttca
     5281 gcctggcgcc gatcaccctg gaccggccgt tcttctatgc cgtgctgcgg ctggaccagc
     5341 tcggcacgat cctccagcgg ctggagatcc tgccgcagca ggagatcggg gcactggtga
     5401 acctggcggt gctggcccag gccgcgc
//
LOCUS       DASSZH010000372         2209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_435025, whole genome shotgun sequence.
ACCESSION   DASSZH010000372 DASSZH010000000
VERSION     DASSZH010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435025"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VFN42_08140"
     CDS             <1..422
                     /locus_tag="VFN42_08140"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET8996623.1"
                     /translation="AVFAQFELEGGILASVNTSWCSRVRREDAMILQVDGTEGSAVAT
                     AQECWIQPDAATPLASMAVDAGQRQDYRAQWVAVPDRGPRINSYRAGWEAFLRHVLDG
                     APFASPLLAGAKGVQLADLAYRSHRERRWIEVPTLAL"
     gene            complement(468..1562)
                     /locus_tag="VFN42_08145"
     CDS             complement(468..1562)
                     /locus_tag="VFN42_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HET8996624.1"
                     /translation="MDSFSTCWDELEDPRTGNAQLHDFHDLLMIALCTVLCGGQTATD
                     MALFARAKETYLRGFLKLPAGLPSHDTFSRLFRLLDPVKFSAWFQRFTAAFGAQLRGV
                     IAVDGKVLRRSHNKAKGISALHMVSAWGCEASLVLAQIATDTKSNEITAVPKLLAMLS
                     LKGTIVTADALNCQRAIAAQIVEQGGDYALPLKENQPSLLADVSLFLDDPTTSPDDRH
                     QSVDADHGRIETRTATVSTAIGWLTETQKWPGLRAIGKVVREREIDGKTSAETAYYVL
                     STPMSAERFNQVVRSHWQVENRLHWRLDVVMNEDQARNRAGHGAHNLAVLRHIALNLM
                     QKDGTKGSIRGKFKRAGWNESYLNGLLALL"
     gene            1754..>2209
                     /locus_tag="VFN42_08150"
     CDS             1754..>2209
                     /locus_tag="VFN42_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010502649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET8996625.1"
                     /translation="MRVLVVGSGGREHALCWALAASPLLTKLYCAPGNPGIGELAECV
                     KIGATDIAELVAFAQEHAIDLVVPGPEAPLSAGIADAMEDAAIACCGPTRDAARLESS
                     KSFTKQICDEAGIPTAAWERFEDAEAARDFVRRRGVPLVVKADGLAAGKG"
BASE COUNT          334 a          708 c          795 g          372 t
ORIGIN      
        1 atgctgtgtt cgcgcagttc gagctggaag gcggcatcct ggccagcgtg aacacctcct
       61 ggtgctcgcg cgtccggcgc gaggacgcga tgatcctgca agtggacggc accgaaggct
      121 ccgccgtcgc caccgcgcag gagtgctgga tccagccgga tgccgcgacg ccgctggcct
      181 cgatggcggt cgatgccggc cagcgccagg attaccgcgc gcaatgggtg gcggtgcctg
      241 accgcgggcc gcggatcaac agctaccgcg ccggctggga agcgttcctg cgccacgtgc
      301 tggacggcgc gccattcgcc tccccgctgc tggccggggc caagggggtg caactggccg
      361 acctcgctta tcggagccat cgggagcggc gctggatcga ggtgccgacg ctcgcgctct
      421 gatccccgcg gcgggtgggg gtggcgcggc ggcagggcaa tcgcatttca gagcagcgcc
      481 aataacccat tgagatagct ttcattccat ccggctcgct tgaattttcc gcggatagat
      541 cccttggttc cgtccttttg catgaggttg agcgcgatat gccgcagcac ggcgaggtta
      601 tgcgcgccgt gtccggcccg gttcctggcc tgatcctcgt tcatcaccac gtccagccgc
      661 cagtggaggc ggttttccac ctgccagtga gagcggacca cctggttgaa ccgctctgcc
      721 gacatcggcg tgctgagcac gtagtatgcg gtctctgcgc tggtctttcc gtcgatctcg
      781 cgttcgcgca ccaccttgcc gatcgcgcgc agcccgggcc atttctgggt ttcggtgagc
      841 cagccgatcg cggtggaaac ggttgcggtg cgggtctcga tccggccgtg atcggcatcg
      901 acgctctggt ggcgatcgtc ggggctggtg gtgggatcat cgaggaacag gctgacatcg
      961 gccagcaggc tgggttggtt ttccttgagc ggcagggcgt agtcgccgcc ttgttcgacg
     1021 atctgggcgg cgatggcgcg ctgacagttg agcgcgtcgg ccgtgacgat ggtgcccttg
     1081 agggagagca tcgccagcag cttgggcacg gcggtgatct cgttcgactt ggtgtcggtg
     1141 gcgatctggg ccagcaccag gctggcctcg cagccccagg cgctgaccat gtgcagggcc
     1201 gagatacctt tggctttgtt gtgggagcgg cgcaggacct tgccgtccac cgcgatgacg
     1261 ccgcgcagct gggcgccgaa ggcggcggtg aagcgctgga accaggcact gaacttcacc
     1321 ggatcgagca ggcgaaacag ccgcgagaag gtgtcgtggc tgggcaggcc ggcgggcagc
     1381 ttgaggaagc cacgcaggta ggtctccttg gcgcgggcaa acagggccat gtcggtcgcg
     1441 gtctgcccgc cacacagcac ggtgcacagc gcgatcatca gaagatcgtg gaaatcgtgg
     1501 agttgggcgt tgccggtccg ggggtcttcg agctcgtccc agcaggttga gaaggagtcc
     1561 atagcagcct cacaaggggg ggatgggctg ccgggtagaa tcgatccggg acgaatcggg
     1621 caagggtgat tcgggggacg tcaagaactt tccaagtgcg agtcccctgc ccgtcaggtc
     1681 cccgtccctc gcctgcccgc cccggttcgt ggcatcatcg ccccgccggt caaccgggcg
     1741 gaggagacag cggatgcgcg tgctggtggt cggatcgggc gggcgcgagc acgccctctg
     1801 ctgggcgctc gccgccagcc cgctgctgac caagctctat tgcgcccccg gcaatccggg
     1861 catcggcgag ctcgccgaat gcgtgaagat cggcgccacc gacatcgccg agctggttgc
     1921 cttcgcccag gaacacgcga tcgatctggt ggtccccggc cccgaggccc cgctcagcgc
     1981 cgggatcgcg gacgcgatgg aagacgcggc catcgcctgc tgcggcccga cccgggacgc
     2041 cgcccggctg gaaagcagca agagcttcac caagcagatc tgcgacgagg ccggcatccc
     2101 caccgccgcc tgggaacgct tcgaggacgc cgaggccgcg cgcgacttcg tccgccgccg
     2161 cggcgtgccg ctggtggtga aggcggacgg cctggccgcc ggcaagggc
//
LOCUS       DASSZH010000373         5455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_264625, whole genome shotgun sequence.
ACCESSION   DASSZH010000373 DASSZH010000000
VERSION     DASSZH010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5455
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264625"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..569
                     /locus_tag="VFN42_08155"
     CDS             87..569
                     /locus_tag="VFN42_08155"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8996626.1"
                     /translation="MKIPVPVTLTVTNGSDIQQAGEVIQSMEREAGFNVKLQAMEFAS
                     SLHAAYGGDFQAYLIGWSGRADPDGNMWQMLHTGGTFNYGHYSNPGVDKALDDARTVA
                     GLEQRRAFYAKVWAQQRKDLPLIYLWTGKNIVGMKKTLTGFRQVPDGLIRLQGMEMQQ
                     "
     gene            618..2246
                     /locus_tag="VFN42_08160"
     CDS             618..2246
                     /locus_tag="VFN42_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HET8996627.1"
                     /translation="MIDPITIAVLQNRLNAIAEEMGEAMLRTAYSQILNSSRDFSIAL
                     IDARCRLVAQADHIPVHVGAMPWAAKALAERFPDPSPGDVYLLNDPYRGGSHLPDLTA
                     FVPVFDGARLMFWSVVRAHHSDIGGATHGAYNPAATEIWHEGLRIPPMRLTENGVLRE
                     DLLDMLALNVRHPRDFRGDLAAQIGAAKLGEQRLKSVIAEFGGAVLADAVEAMLDAAE
                     RHAREIVAGWRDGVYDGEAVLDDDGFGKTDIVIRARVTKQGSDVTVDLTQSDPQVTGF
                     INSSHANTQSAVAMAFAFLLDPDITKNEGAFRPLTVKLKEGTIVWAREGAPVTMCTSH
                     CSNEIIEAIIVALAPACPERAMGGWGRRLRIALSGVDPRNGRRFIWHMFQARPGGGGS
                     AAGDGFSTIGEWHSAGGIKFGSIEVAETRFPLVFETHEFRPGSAGDGRYRGGYGGDMR
                     LRVETDGAAVANTAGEGVVHGARGILRGRDGAPHDYTLLAPGAAPRKLKSKEVGVVVP
                     SGSVIHVLSGGGGGWGSPEQRDPAARKRDAAEGL"
     gene            complement(2272..2982)
                     /locus_tag="VFN42_08165"
     CDS             complement(2272..2982)
                     /locus_tag="VFN42_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8996628.1"
                     /translation="MSLLRIEDLSVAYGQIEALKGVSLAVEKGEVVAILGANGAGKTT
                     LMRAISSLIGKRGGRILFEDHDVTRAPADRIVRMGIAQSPEGRRVFGTLSVAENLALG
                     GFTRPAGEIRESLEFAYRMFPRLAERKSQFAGTLSGGEQQMLAIARALVAKPKLLLLD
                     EPSLGLAPIIVQGIFRTLRQIADTGVTILLVEQNARSALKLADRGYVLEVGRIVLQDT
                     AARLLASPEVQAAYLGKA"
     gene            complement(2979..3758)
                     /locus_tag="VFN42_08170"
     CDS             complement(2979..3758)
                     /locus_tag="VFN42_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8996629.1"
                     /translation="MSALLELRSIGMTFGGLRAVDELGFAVQEGQIVGLIGPNGAGKT
                     TVFNIITGVYRPTDGDVFWQGSRITGAAPHRVARLGIRRTFQTIRLFPEMSVFENVRA
                     GCHLRAHQHWWQGMLALPGQRREETALAERALDILRRLDLLQVADEPATSLPYGMQRR
                     VELARTLAGEPRLVILDEPAAGLNDAESAALNDTIFAIRDSGITVLLVEHDMNVVMNV
                     TDHIVVINFGRKIAEGNPEAIRTNPAVIEAYLGTDDEETAA"
     gene            complement(3755..4738)
                     /locus_tag="VFN42_08175"
     CDS             complement(3755..4738)
                     /locus_tag="VFN42_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8996630.1"
                     /translation="MAAPLDAAGTAPSVSATRRPRFRLAEAIGIALWILGLTIPGFLD
                     STWVSVAAIFAIYAITALSEDVILGRGGMFDMGHAVYLGLGAYATGILNTELGWPIFA
                     TMPAAILLAAAFGALLAAPLIRLRGDYLLVATIGFNAIFILAAENNLFGITGGSDGLF
                     GLDVPRLFGMQLASQSAMFWLDWIVLGFVLLLMRNLDRCELGRTLRYLKQDELAATTL
                     GVDARAAKTLAFAIGAGLAGLAGSLFAVQLSSVGPSSFVFTESVTLFAIVIVGGQGSI
                     PGVLLGTALMFVVPQIFRDLAEYRYFAFGFAMIAVMVLRPQGLWPRRRVAA"
     gene            complement(4738..>5455)
                     /locus_tag="VFN42_08180"
     CDS             complement(4738..>5455)
                     /locus_tag="VFN42_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8996631.1"
                     /translation="LYLFVHHTRFGTAIRAAAIDQDAARLMGINVNRVIASVFVIGPA
                     LGASVLIENGMMLIWGANVHVFPSGLLPDTSWNVVGATITFVQVMIIIVSIVLMAALY
                     VFVHHTRFGTAIRAAAIDQDTARLMGINVNRVIASVFVIGPALGAIGGLFVGIYYREA
                     YFTMGWTYGLYAFIAAILGGIGNIPGAMLGGVLLGLFNAFAAGYVSSSWQDAITFGLL
                     IAILLVRPTGILGERVAEKV"
BASE COUNT          994 a         1754 c         1858 g          849 t
ORIGIN      
        1 ccgccagctc gccgtattat ttcaaggata tggcgccgcc gaagcgcgat cccgccaagg
       61 cgatggcgct gctgaagtag gcgggggtga agattccggt gccggtgacg ctgacggtca
      121 ccaacggctc cgatatccag caggccggtg aagtgatcca gtccatggag cgggaagccg
      181 gcttcaacgt gaagctgcag gcgatggaat tcgcctcgtc cctgcacgcg gcctatggcg
      241 gggatttcca ggcctatctg atcggctggt ccgggcgggc cgacccggac ggcaatatgt
      301 ggcagatgct gcacactggg ggcaccttca actacgggca ctacagcaat cccggcgtgg
      361 acaaggcgct ggatgacgcg cgcaccgtcg ccggcctgga gcagcggcgg gcgttctacg
      421 ccaaggtctg ggcgcagcaa cgcaaggacc tgccgctgat ttacctgtgg accggcaaga
      481 acatcgtcgg gatgaagaag acgctgacag gcttccgcca ggtgccggat gggctgatcc
      541 ggctgcaggg gatggagatg cagcaataga gggggcggcc ggcttccctg acgccccgcg
      601 tctgctagca ttggcggatg attgacccca tcaccatcgc cgtgctgcag aaccggctga
      661 acgccatcgc cgaggaaatg ggcgaggcga tgctgcgcac ggcctattcg cagatcctga
      721 attcctcgcg cgatttctcc atcgcgctga tcgatgcgcg ttgccgcctg gtcgcgcagg
      781 cggaccatat tccggtgcat gtcggcgcca tgccctgggc ggcgaaggcg ctggcggagc
      841 ggtttcccga tccatcgccg ggcgatgtgt acctgctgaa cgacccgtat cgcggcggct
      901 cgcatctgcc tgacctgact gctttcgtgc cggtgttcga cggcgctcgg ctgatgttct
      961 ggtcagtggt gcgggcacac cactccgata tcggtggcgc aacgcacggc gcctacaatc
     1021 ccgccgccac ggaaatctgg cacgagggtc tgcgcatccc gcccatgcgc ctgaccgaga
     1081 acggcgtgct gcgcgaggat ctgctggaca tgctggcgct gaacgtgcgc catccgcgcg
     1141 acttccgtgg cgacctcgcc gcacagatcg gggcggcaaa actcggtgag cagcggctga
     1201 aatcggttat tgccgagttc ggcggtgccg tgctggccga tgcggtggaa gcgatgctcg
     1261 acgccgccga acggcatgcg cgggaaattg tcgctggatg gcgcgacggt gtgtatgacg
     1321 gcgaagccgt gctggacgac gacgggttcg gcaagaccga tatcgtcatc cgcgcgcggg
     1381 tcaccaagca gggcagcgac gtaaccgtgg atctgacgca gtccgatcca caggtgaccg
     1441 gattcatcaa ctcctcgcat gccaacacgc aatccgcggt ggcgatggca tttgcgttcc
     1501 tgctcgaccc cgacatcacc aagaacgagg gcgcattccg gccgctgacg gtgaagctga
     1561 aggaaggcac catcgtctgg gcgcgtgagg gtgcgccggt gacgatgtgc accagccact
     1621 gctccaacga gatcatcgaa gcgatcattg ttgcactggc acccgcctgt cccgagcggg
     1681 cgatgggcgg ctgggggcgg cggctgcgca tcgcgctgag cggtgtcgat ccacgcaatg
     1741 ggcgccgctt catctggcac atgttccagg ctcggcctgg cggtggcgga tcggcggcgg
     1801 gcgacgggtt ctccaccatc ggtgaatggc actccgccgg cggcatcaag ttcggctcga
     1861 tcgaagtggc ggagacgcgc ttcccgctgg tgttcgagac gcacgaattc cgtccaggct
     1921 ccgcgggcga tggcaggtac cgcggcggct atggcggcga catgcggctg cgcgtggaaa
     1981 ccgatggcgc cgcggtggcg aataccgccg gcgagggagt ggtgcatggc gcgcgcggca
     2041 ttctgcgggg acgggacggc gcaccgcacg attacacgct gcttgctcca ggcgctgcgc
     2101 cacgcaagct gaaaagcaaa gaggtcggcg tggtagtgcc cagcggaagc gtgatccacg
     2161 tgctgtccgg tggtggcggt ggctgggggt cgccggagca gcgcgacccc gcggcgcgga
     2221 aacgggatgc ggcggaaggg ctgtagggaa cccgcgcgac ggcgttgaat gtcaggcctt
     2281 ccccagatag gccgcctgta cctccggtga cgcgagcagc cgcgcggcag tgtcctgcag
     2341 cacgatgcgg cctacctcca gcacatagcc gcgatccgcc agcttcagcg cggaccgtgc
     2401 gttctgttca accagcagga tcgtcacgcc ggtatcggca atctgccgca gggtgcggaa
     2461 aatcccctgc acaatgatcg gcgccagccc caggctgggc tcatccagca gcagcagctt
     2521 cggcttcgcc accagcgcgc gcgcgatcgc cagcatctgc tgctcgccgc cggacagcgt
     2581 gccggcgaac tggctcttgc gttccgccag ccgcggaaac atgcggtagg cgaattccag
     2641 gctttcgcgg atctcacccg cgggccgggt gaagccgccc agcgccaggt tctcggcgac
     2701 gctgagggtc ccgaacacgc gccgcccttc cggagattgc gcgatcccca tgcgcacgat
     2761 gcggtccgcc ggggcgcgcg tgacgtcatg gtcttcaaac aggatgcggc cgccgcgctt
     2821 gccgatcagg ctggagatgg cacgcatcag cgtcgtcttg ccggcgccgt tggcgccgag
     2881 gatggcgaca acttcgccct tctccacagc cagagacacg cccttcagcg cctcgatctg
     2941 accataggcg acggacaggt cctcaatgcg gagcaggctc atgccgccgt ctcctcatcg
     3001 tccgtaccga gataggcttc gatcaccgcc ggattggtgc ggatcgcctc cggattgccc
     3061 tcggcaattt tgcggccgaa attgatcacc acgatgtggt cggtgacgtt catcaccacg
     3121 ttcatgtcgt gctcgacgag aagtacggtg atgccggaat cgcggatggc gaagatcgtg
     3181 tcgttcagcg cggcggattc ggcatcgttc aggccggcgg ctggctcatc caggatcacc
     3241 aaacgcggct cgccggccag cgtacgggcc agctccacgc ggcgctgcat gccgtagggc
     3301 agcgacgtcg caggctcgtc cgccacctgc agcagatcga gccggcgcag gatgtcgagc
     3361 gcgcgctccg ccagcgcggt ctcctcgcgc cgctgccccg gcaacgcgag catgccctgc
     3421 caccaatgct gatgcgcgcg cagatggcag ccggcgcgta cgttctcgaa taccgacatc
     3481 tcgggaaaca ggcggatggt ctggaacgtg cggcggatac ccagccgcgc cacgcgatgc
     3541 ggcgccgcgc cggtgatgcg tgagccctgc cagaacacgt cgccatcggt cgggcgatac
     3601 acgcccgtga tgatgttgaa caccgtggtc ttgccggcac cgttcgggcc gatcagtccg
     3661 acgatctggc cttcctgcac cgcgaacccg agctcgtcca ccgcgcgcaa tccgccgaac
     3721 gtcatgccga tgctgcgcag ttccagcagc gcgctcatgc cgccaccctc cgccgcggcc
     3781 acaggccttg cgggcgcagc accatcaccg cgatcatggc aaagccgaag gcgaagtagc
     3841 ggtactccgc gaggtcgcga aagatctgcg gcaccacgaa catcagggcg gtgccgagca
     3901 gcacgccagg gatggagccc tggccgccga cgatgacgat ggcgaacagc gtcaccgatt
     3961 cagtgaatac gaaggaagac ggaccgacgc tggagagttg tacggcgaac aggctgcccg
     4021 ccagccccgc caatccggcg ccgatcgcaa aggccagggt ttttgccgcg cgtgcatcga
     4081 cgccgagcgt ggtggcggcg agttcgtcct gcttcaggta gcgcagcgtg cggccgagtt
     4141 cgcagcggtc cagattgcgc atcagcagca gcacgaagcc cagcacgatc caatccagcc
     4201 agaacatcgc agactggctg gcgagctgca tgccaaacag gcgcggcacg tccaggccga
     4261 acaggccgtc cgatccgccg gtgatgccga acaggttgtt ctcggccgcc agaatgaaga
     4321 tcgcattgaa gccgatggtg gcgaccagca ggtagtcgcc gcgcaaccgg atcagcggcg
     4381 ccgccagcaa cgccccgaat gccgcggcga gcaggatcgc cgccggcatt gtggcgaaga
     4441 tgggccagcc gagctcggtg ttcaggatgc cggtcgcgta ggcgccgagg ccgagatata
     4501 ccgcgtggcc catatcgaac atgccgccac ggcccaggat cacgtcttcc gacaatgcgg
     4561 tgatggcgta gatcgcgaag atggcggcga cggataccca ggtgctgtcg aggaatcccg
     4621 gaatggtcag gccaaggatc cacagcgcga tgccgatcgc ttccgccaac cgaaaccggg
     4681 gccggcgcgt ggcagaaaca ctgggggcgg tgccggcggc gtccagcggt gcagccatca
     4741 gaccttctcc gccacgcgtt cgccgaggat acccgttggg cgcaccaaca ggatggcgat
     4801 cagcaggccg aaggtgatgg cgtcctgcca ggagctggac acgtagccgg ccgcaaacgc
     4861 gttgaacaag ccaagcagca cgccgcccag catggcgccg ggaatgttgc caatgccgcc
     4921 gagaatggcg gcgatgaagg catacagccc ataggtccag cccatggtga agtaggcttc
     4981 gcggtagtag atgccgacga acagcccgcc gatcgcaccc agcgcggggc cgatgacgaa
     5041 tacgctggcg atcacgcgat tgacattgat gcccatcagt cgggcggtgt cctggtcgat
     5101 cgccgccgca cggatcgcgg tgccgaatcg cgtgtggtgc acgaacacat acagcgccgc
     5161 catcagcacg atggagacga tgatgatcat cacctgcacg aaggtgatgg tggcgccgac
     5221 cacattccaa ctggtgtccg gcagcaggcc ggaagggaac acgtgcacgt tggcgcccca
     5281 gatcagcatc atgccgttct cgatcagcac ggaggcgccg agcgccgggc cgatgacgaa
     5341 cacgctggcg atgacgcggt tgacgttgat gcccataagc cgcgcggcgt cctggtcgat
     5401 cgccgcggcg cggatcgcgg tgccgaagcg ggtgtggtgc acgaaaaggt acagc
//
LOCUS       DASSZH010000374         2098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1270170, whole genome shotgun sequence.
ACCESSION   DASSZH010000374 DASSZH010000000
VERSION     DASSZH010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270170"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VFN42_08185"
     CDS             <1..613
                     /locus_tag="VFN42_08185"
                     /inference="COORDINATES: protein motif:HMM:NF016310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HET8996632.1"
                     /translation="LAGDYWQVPLIAGLDRVPNSDEMKHFGAAAASFGSLALFHMDGV
                     TPEARAGAAGSGGNLPVHRVGQADIRSFQASYAGGEGGADLVVFSAPQLSLVEMQQVA
                     GLLDGRQAQIPLLVITSPQVKPDADRMGITGRIEAAGGTVLAGMCFYNSYAREMAEAN
                     GWRRLATNSAKLVNIIGGYGYRPALLSMEACIDTACTGKIAHV"
     gene            606..1061
                     /locus_tag="VFN42_08190"
     CDS             606..1061
                     /locus_tag="VFN42_08190"
                     /inference="COORDINATES: protein motif:HMM:NF014091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HET8996633.1"
                     /translation="MSELTLHPRAAMGPRVRGPALVAKDGFSARYDLDRIQGTFSRPE
                     HKLAGENYVGRVLVLDVAKGGVATAWMLYEMASRGMAPAALVLNVANPIMVQGAALAG
                     ITLVSGFDGDITALVPPGAEVEIDPQASPPTLRVVTAGQQAERAADAAG"
     gene            1242..1991
                     /locus_tag="VFN42_08195"
     CDS             1242..1991
                     /locus_tag="VFN42_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HET8996634.1"
                     /translation="MKVLVPVKRVVDYNVKVRVKADGSGVETAGVKMSMNPFDEIGVE
                     EAVRLKEKGVATEIVAVSLGVAQCQETIRTALAMGADRGILVETDVELQPLAVAKLLK
                     AIADQEQPKLIILGKQAIDDDMSSTGQMLAALLGWPQATFASKVTIEGDDITVTREVD
                     GGLETVALKLPAIVTTDLRLNEPRYASLPNIMKARKKPIATMKPDELGVDPTPRLTTL
                     KVSEPPKRKAGVKVGSVAELVQKLKTEAKVI"
     gene            1994..>2098
                     /locus_tag="VFN42_08200"
     CDS             1994..>2098
                     /locus_tag="VFN42_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008491962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HET8996635.1"
                     /translation="MTALVLLEFDDAGVKQPSRSAVAAAQKLGDVHVLV"
BASE COUNT          362 a          661 c          741 g          334 t
ORIGIN      
        1 gctggccggc gattactggc aggtgccgct gatcgccggg ctggaccgtg tgcccaactc
       61 cgacgagatg aagcatttcg gcgccgccgc ggcgagtttt ggctcgctcg ccctgttcca
      121 catggacggg gtaacgccgg aggcgcgcgc cggtgcggcg ggctccggcg gcaatctgcc
      181 ggtgcaccgc gtcggccagg ctgacatccg cagcttccag gcgagctatg ccggggggga
      241 aggcggcgcc gatctggtgg tgttctccgc cccccagttg agtctggtgg agatgcaaca
      301 ggtggccggc ctgctggacg ggcggcaggc gcagatcccg ttgctggtga tcaccagtcc
      361 gcaggtgaag ccggatgccg accgcatggg catcaccggc cggatcgaag ccgccggcgg
      421 caccgtgctc gccggcatgt gcttctacaa ctcctacgca cgtgaaatgg cggaggcgaa
      481 tgggtggcgg cgtctggcca ccaattcggc aaagctggtg aacatcatcg gcggctacgg
      541 ctatcgccct gcgctgctgt cgatggaagc ctgtatcgac accgcttgta ccgggaagat
      601 cgcccatgtc tgaactgaca ctgcatccgc gcgctgccat gggaccgcgc gtgcgcgggc
      661 cggcgctcgt cgccaaggac gggttttccg cgcgctacga cctggaccgc atccagggca
      721 cattctcccg gccggaacac aagctggcgg gggagaacta tgtcggccgg gtactcgtgc
      781 tggatgtggc gaagggcggc gtggccaccg cctggatgct gtatgagatg gcatcgcgcg
      841 gcatggcacc cgcggcactg gtcctgaacg tcgccaatcc gatcatggtg cagggcgcgg
      901 cgttggcagg catcaccctg gtttccggtt tcgacgggga tatcaccgcg ctggtgccgc
      961 cgggcgccga ggtggagatc gacccgcaag ccagtccgcc aaccctgcgg gtggtgaccg
     1021 ccggccagca ggcggaacgg gcagcggatg ccgccggctg accggatttg ccggcttcaa
     1081 tcctgcggca accgccggtt acccacgagt atgaccggcg tttgacaggg gtgcgccccc
     1141 tgcatatggt cccggcgctc tattgcaagt gcgaacaggc ggccaatgcg ccgggcctgt
     1201 agcgcaggtg tgtgttcccc agcagcggca cggttttccc catgaaggtc cttgtccccg
     1261 tcaagcgggt ggtcgactac aacgtgaagg tccgtgtgaa agcggacggt tccggcgtgg
     1321 aaaccgccgg agtcaagatg tccatgaacc cgttcgacga gatcggcgtc gaggaggcgg
     1381 tccgcctgaa ggaaaagggc gtcgccaccg agatcgtcgc cgtctccctg ggcgtcgcgc
     1441 agtgccagga gacgatccgc acggcactgg ccatgggcgc cgaccgtggc atcctggtgg
     1501 agaccgacgt cgaactgcag ccgctggccg tcgccaagct gctgaaggcg attgccgatc
     1561 aggaacagcc aaaactgatc atcctgggca agcaggcgat cgacgacgac atgtcctcca
     1621 ccggccagat gctcgccgcc ctgctcggct ggccgcaggc caccttcgcc agcaaggtca
     1681 ccattgaagg cgacgatatc accgtcaccc gcgaagtgga tggcgggctg gagacggtgg
     1741 cgctgaagct gccggccatc gtcaccaccg acctgcggtt gaacgagccg cgttacgcct
     1801 cgctgcccaa catcatgaag gcgcgcaaga agccgattgc cacgatgaag ccggacgagt
     1861 tgggcgtcga tccgacgccg cggctgacca cgctgaaggt cagcgagccg ccgaagcgca
     1921 aggcgggggt gaaggtcggc tcggtggccg agctggtgca gaagctgaag accgaagcga
     1981 aggtgatctg acgatgacgg ctcttgtcct gctggaattc gacgatgccg gcgtgaagca
     2041 gccgtcgcgc agcgcggtgg ccgcagcgca gaagctgggc gacgtgcacg tgctggtc
//
LOCUS       DASSZH010000375         3672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_128297, whole genome shotgun sequence.
ACCESSION   DASSZH010000375 DASSZH010000000
VERSION     DASSZH010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128297"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VFN42_08205"
     CDS             <1..964
                     /locus_tag="VFN42_08205"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF006589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HET8996636.1"
                     /translation="LWPSWRLQGDDLAEGKRDATLLALKLQEDAGIDIVTEGEQSRQH
                     FVHGFLERIDGIDFEHRVEMGIRDNRYKAMVPTVTAPLRLTDRVHATEARIARAHTTR
                     KLKFTLPGPMTIVDTIADTHYGDKVKLAMAFAALLNQEARALEADGVDVIQFDEPAFN
                     VYMPEVATWGITALERAAEGLRCQTAVHICYGYGIQANIDWKQSLGEEWRQYEAIFPV
                     LARSRIGQVSLECRNSRVPMRLLRLLDGKDVLAGVIDVATDTVETPEDVATLIEDLAE
                     HVPVERLFPCTNCGMAPMRREIAEAKLMALGRGTALARQRLQGK"
     gene            1005..1787
                     /locus_tag="VFN42_08210"
     CDS             1005..1787
                     /locus_tag="VFN42_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase/reductase"
                     /protein_id="HET8996637.1"
                     /translation="MDLQLRGKTALITGGSKGIGYASAEALAEEGVNVILVSRTEADL
                     HAARARIAGRCNVRTEIHAYDIADSRNVDRLAEQHPDIDILVNNAGAIPGGNLQDIDE
                     ARWRAAWDLKVYGYINMCRRFYAAMKQRGRGTIINILGMAGERMDSGYIAGSTGNAGL
                     MAFTRTLGGAASSDGLRVVGINPGAVATDRLVTLMQKRAEDRFGDASRWTELMAPLPY
                     GRAGKPEEIGWMVAFLASDKSAYTTGTIITIDGGAANRGPMF"
     gene            1848..2252
                     /gene="apaG"
                     /locus_tag="VFN42_08215"
     CDS             1848..2252
                     /gene="apaG"
                     /locus_tag="VFN42_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HET8996638.1"
                     /translation="MAEDYAKTTRGVRITVRSFYLEDQSQPEENHYVWAYRVKIENNS
                     RQAVQLLRRTWHITDARGRTQHVHGAGVVGEQPLLEPGESFEYTSGTPLETPSGFMVG
                     AYHMVIPASGENFDVAIPAFSLDSPHQSGRVH"
     gene            2341..2982
                     /gene="folE"
                     /locus_tag="VFN42_08220"
     CDS             2341..2982
                     /gene="folE"
                     /locus_tag="VFN42_08220"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET8996639.1"
                     /translation="MNIVTPPPNRTTDASTLKRPTREEAEQAVRVLLRWAGDDPTREG
                     LIDTPGRVARAYEEFFAGYDVDPVALLERTFEETDGYDEIVLLRDIRLESHCEHHMVP
                     IIGRAHVAYLPHRRVVGISKLARVVEAYAKRFQIQEKMTAQIANTIQQVLEPRGVAVV
                     IEAAHQCMTTRGVHKPGVTMVTSRMLGAFRDNPATRRELLAMINNRGTSITGD"
     gene            complement(2993..>3672)
                     /locus_tag="VFN42_08225"
     CDS             complement(2993..>3672)
                     /locus_tag="VFN42_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996640.1"
                     /translation="LLPLLLTACGGGEPRLFPPLSFRRLPPLRLNVARIEIEQHFFPS
                     GMTPDVTQVDPVPPIDALRAMANDRLKAFGSTGRAVFIIQNASLTKQDDTITGNMDVV
                     LNVYPSDTGPRAGFAEARVSRQYTGDTDDLRQRLYDMTKAMMQAMNVEFEYQVRNRLH
                     DWMVSGNAAVPPVQAQPLNAGPEPGTPPAPNVPPPGAPALPSMPPPSAPPPTSLPPPI
                     PMPPGRS"
BASE COUNT          661 a         1208 c         1247 g          556 t
ORIGIN      
        1 gctttggccg tcctggcgtc tgcagggtga cgatctggcg gagggcaagc gcgatgccac
       61 cctgctggcg ctcaagctgc aggaagatgc cggcatcgac atcgtcaccg aaggcgagca
      121 gtcgcgccag catttcgtgc acggcttctt ggaacggatc gacgggatcg atttcgaaca
      181 ccgcgtggag atgggcatcc gcgacaatcg ctataaggcg atggtgccga ccgtcacggc
      241 gccgctgcgc cttacggacc gcgtgcatgc gaccgaggcg cgcatcgcgc gcgctcacac
      301 gacacgcaag ctcaagttca ccctgccggg gccgatgacg atcgtggata cgattgccga
      361 cacccattac ggcgacaagg tgaagctggc gatggcattc gccgcgctgc tgaaccagga
      421 agcgcgcgcg ctggaggccg acggagtgga cgtgatccag ttcgacgaac ccgccttcaa
      481 cgtctacatg cccgaagtcg ccacctgggg catcaccgca ctggagcgcg ccgcggaagg
      541 actgcgctgc cagaccgccg tgcacatctg ctacggctac ggcatccagg cgaacatcga
      601 ctggaagcag tcgctcggcg aggaatggcg ccagtacgag gcgatcttcc cggtgctggc
      661 acgcagccgc atcgggcagg tctcgctgga atgccgcaat tcccgcgtgc cgatgcggct
      721 gctgcggctg ctcgacggca aggacgtgct ggcaggcgtg atcgacgtgg cgacggatac
      781 cgtggagacg ccggaagacg tcgccacgct gatcgaggac ctggccgaac acgtgccggt
      841 ggagcggctg ttcccgtgca cgaattgcgg catggcgccg atgcgccgcg agatcgcgga
      901 agccaagctg atggcactgg gccgtggcac cgcgctggcg cggcagcggc tgcaagggaa
      961 atagacgggg gcgcaagccc cgcacgcaac aaggagaatg ggtcatggac ctgcagcttc
     1021 gcggcaagac ggcgctgatc accggcggat cgaagggcat cggctacgct tccgccgaag
     1081 cgctggccga ggagggcgtc aacgtcatcc tggtctcccg caccgaagcc gatctgcacg
     1141 cggcgcgcgc gcgcatcgcc ggacgctgca acgtgcgcac ggagatccac gcctatgaca
     1201 tcgccgacag ccgcaatgtc gatcgcctgg cggagcagca ccccgatatc gacattctgg
     1261 tgaacaatgc cggcgcgatc cccggcggca acctgcagga catcgacgaa gcgcgctggc
     1321 gcgcggcgtg ggacctgaag gtctacggct acatcaacat gtgccgccgc ttctacgcgg
     1381 cgatgaagca gcgcggccgg ggcaccatca tcaacatcct cggcatggcc ggcgagcgca
     1441 tggattccgg ctatatcgcc ggttccaccg gcaatgccgg cctgatggcc ttcacccgga
     1501 cgctgggcgg cgcggcttcg agtgacgggc tgcgggtggt cggcatcaat ccgggagccg
     1561 tggcgaccga ccggctggtg acgctgatgc agaagcgcgc cgaggaccgg ttcggcgacg
     1621 caagccgctg gaccgagctg atggcccccc tgccctacgg ccgcgcgggc aaaccggagg
     1681 agatcggctg gatggtggcg ttcctggcgt cggacaaatc ggcctatacc accggcacga
     1741 tcattacgat cgacggcggc gcggccaatc gcgggcccat gttctgaccg ccggccggcg
     1801 tttccgttcg ggtggacaac gcaagccggt tgtccgagga ttgaagcatg gcggaagatt
     1861 acgcgaaaac cacccgtggc gtccgcatca ccgtgcggtc cttttacctc gaggaccagt
     1921 cgcagccgga agagaaccac tatgtctggg cgtatcgcgt aaaaatcgag aacaacagcc
     1981 ggcaggcggt gcagttgctg cgccgcacct ggcacatcac cgacgcacgc gggcgcacgc
     2041 agcatgtgca tggcgccggg gtggtcggcg agcagccgct gctcgagccg ggagaaagct
     2101 tcgaatacac ctccggcacg ccgctggaga cgccgtccgg cttcatggtt ggcgcctatc
     2161 acatggtaat tccggcgtcc ggcgagaatt tcgacgtcgc catccccgcc ttcagtctgg
     2221 acagcccaca ccagagcggt agggtccact agaccgcgcg gggttgtgag cgtcccgtgg
     2281 ggtcccatat cggcggcggg ccaaccgcca cggcaatcgc actccggagg aactcccgcg
     2341 gtgaatatcg ttacacctcc ccccaaccgc accacggacg cctcgaccct gaagcgaccg
     2401 acccgcgagg aagccgaaca agcggttcgc gtcctgctgc gctgggccgg ggacgatccc
     2461 acccgcgagg gtctgatcga cacgccgggc cgcgtcgcgc gcgcctatga ggagttcttc
     2521 gccggctacg atgtcgatcc cgttgcgctg ctggagcgca cgttcgagga aaccgacggc
     2581 tacgacgaga tcgtgctgct gcgggacatc cggctggaaa gccattgcga gcaccacatg
     2641 gtgccgatca tcggccgcgc gcacgtggcg tatctgccgc accggcgcgt ggtggggatc
     2701 agcaagctgg cgcgcgtggt ggaagcctat gccaagcgct tccagatcca ggagaagatg
     2761 accgcccaga tcgccaacac cattcagcag gtgctggagc cgcgcggcgt cgccgtggtg
     2821 atcgaggcgg cgcaccagtg catgaccacg cgcggcgtgc acaagcccgg cgtcaccatg
     2881 gtcacttccc gcatgctcgg cgcgttccgc gacaatccgg cgacccggcg ggaattgctg
     2941 gccatgatca acaaccgcgg caccagcatc accggcgact gagccgccgc gatcaggatc
     3001 tgcctggcgg catcgggatc ggcggcggca gcgacgttgg cggcggtgcg ctgggcggcg
     3061 gcatgctcgg cagagccggc gccccgggcg gcgggacatt cggggccggc ggcgtccccg
     3121 gctcgggccc ggcgttcagc ggctgcgcct gcaccggcgg caccgccgcg ttgccactca
     3181 ccatccagtc atgcagccgg ttgcgtacct ggtactcgaa ttccacgttc atcgcctgca
     3241 tcatcgcctt ggtcatgtca tacaggcgct ggcgcaggtc gtcggtgtcg ccggtatact
     3301 ggcggctgac gcgtgcctcg gcgaaaccgg cacgcgggcc ggtgtcggat ggatacacgt
     3361 tcaggaccac atccatattt ccggtgatgg tgtcgtcctg cttggtcagc gatgcattct
     3421 ggatgatgaa cacggcccgc ccggtgctgc caaaggcctt cagccggtcg ttcgccatcg
     3481 ccctcagcgc gtcgatcgga ggcaccggat cgacctgcgt cacgtccggc gtcatgccgg
     3541 aggggaagaa gtgctgctca atctcaattc gggcgacatt caaccggagc ggcggcagcc
     3601 tgcggaagct caatggaggg aagagtctcg gctccccacc gccgcaagcc gttagcaaca
     3661 gcggcagcag ca
//
LOCUS       DASSZH010000376         2264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1645198, whole genome shotgun sequence.
ACCESSION   DASSZH010000376 DASSZH010000000
VERSION     DASSZH010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645198"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VFN42_08230"
     CDS             <1..321
                     /locus_tag="VFN42_08230"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET8996641.1"
                     /translation="SPQVSRILRSLLNRETHIHSVELQRDLVQRLDHRVSLVQRPARH
                     AHFVVLEAANIPSVLIETGFLSNPHDEALLRTRRHQIVVATSIRWAISRYLTEDAKSL
                     RLSS"
     gene            complement(470..1747)
                     /gene="purD"
                     /locus_tag="VFN42_08235"
     CDS             complement(470..1747)
                     /gene="purD"
                     /locus_tag="VFN42_08235"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET8996642.1"
                     /translation="MRVLVIGSGGREHALCWALSASPLLTKLWCAPGNPGIAQVAECV
                     PIGVLDFPALVAFAQDNQIDLVVPGPEAPLVAGITDAMDEAGIACCGPSRAAAQLEGS
                     KSFTKEVCDDASIPTAQWERFDDPAAAREFIRRRGAPLVVKADGLAAGKGVVVAMTEA
                     DALAAVDHIMEDRAFGESGAAVVIEECLTGDEISLFALCDGSTAIPLGSAQDHKRVGE
                     GDTGANTGGMGAYAPAPMFSPELEAACMDRIIQPALAEMARRGTPFRGILFAGLMLTD
                     DGPKLIEFNVRFGDPECQVLLLRLRSDLLPALQAACDGELANFDLRWHDKAALAVVLA
                     ARGYPEAPERGSEIRGLERAAAITGVEVFHAGTEADSAGHIRAVGGRVLTVCALAPSL
                     REASDAAYAAVRTIDWPEGFCRRDIGWRALARA"
     gene            complement(1851..>2264)
                     /locus_tag="VFN42_08240"
     CDS             complement(1851..>2264)
                     /locus_tag="VFN42_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015072411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HET8996643.1"
                     /translation="ESRHNLAYWRYADYAGIGPGAHGRLTRDGVLYAVRRHRAPEPWA
                     ERVERVGHGTVEDALLDANDRAREMLLMGLRLTEGIDPARFLTRTGLALEQATDPGTL
                     RDALEAGYLMWEAGRLRATPEGRLRLDALLAALVR"
BASE COUNT          333 a          822 c          766 g          343 t
ORIGIN      
        1 tccccgcagg tttcccgcat cctgcgcagc ctgttgaacc gcgagaccca catccactca
       61 gtcgaattgc agcgcgatct ggtgcagcga ctggaccatc gcgtcagctt ggtccaacgc
      121 ccggcgcggc acgcgcactt cgtggtgctg gaggcggcga acattccgag cgtcctgatc
      181 gagaccggct tcctgtccaa tccgcatgac gaggcgctgc tgcgcacgcg ccgccatcag
      241 atcgtcgtgg cgacgtccat ccggtgggcc atcagtcgct atttgaccga ggatgcgaag
      301 agcttgcggc tatcaagctg atcgctaccg tccattgcgc cggggaaact gcggcacagg
      361 cgttccctgg cggccgggaa catgcgcctc cggcggaacg tcgcggcgga tttcacccgc
      421 gcggcttggt gcgcatcgcc gccgggctcc atgcgtcagc gccggcacct caggcgcgtg
      481 ccagggcccg ccagccgata tcgcggcggc agaatccctc cggccagtcg atcgtccgca
      541 ccgcggcata ggcggcgtcg gaggcttccc gcagcgacgg cgccagcgcg caaacggtga
      601 gcacgcgtcc gcccacggcg cggatatggc cggcgctgtc cgcttcggtg ccggcgtgga
      661 acacttcgac cccggtgatc gcggccgccc gttccaggcc gcggatttcg ctgccgcgtt
      721 ccggcgcttc cggatagccg cgcgccgcca gcaccaccgc gagcgccgcc ttgtcgtgcc
      781 agcgcaggtc gaaattcgcc agctcgccgt cgcacgccgc ctgcagggcc ggcagcaggt
      841 cggagcgaag ccgcagcagc agcacctggc attccgggtc gccgaagcga acattgaact
      901 cgatcagctt cgggccgtca tcggtcagca tgaggccggc gaacaggatg ccgcggaacg
      961 gcgtgccgcg ccgcgccatt tccgccaggg cgggctggat gatgcggtcc atgcaggccg
     1021 cctccagctc gggcgagaac atcggtgccg gggcataggc gcccatgccg ccggtattcg
     1081 cgccggtatc gccctcgccg acgcgcttgt ggtcctgcgc cgaacccagc gggatggcgg
     1141 tggagccgtc acacagtgcg aacagcgaga tctcgtcgcc ggtcaggcat tcctcgatca
     1201 ccacggcggc accggattcg ccgaacgcgc ggtcttccat gatgtggtcc accgccgcca
     1261 gggcgtcggc ctcggtcatt gcaaccacca cgcccttgcc ggccgccagg ccgtcggcct
     1321 tcaccaccag cggtgcaccg cggcggcgga tgaattcccg ggcggctgcc ggatcgtcga
     1381 aacgctccca ctgcgcggtc gggatgctgg cgtcgtcgca gacttccttg gtgaagctct
     1441 tgctgccttc cagctgagcg gcggccctgc tgggtccgca gcaggcgatg ccggcctcgt
     1501 ccatcgcgtc ggtgatgccg gcgaccagcg gcgcttcggg gccgggcacc accaggtcga
     1561 tctggttgtc ctgcgcgaag gcgaccagcg ccggaaagtc caacacgcca atcggcacgc
     1621 attccgccac ctgggcgatg ccgggattgc ccggggcgca ccacagcttg gtcagcagcg
     1681 ggctggccga gagtgcccag cacagcgcgt gctcacgccc gcccgatccg atcaccagca
     1741 cccgcatgca caagctccca ggctagatcc cgcccaggcc gggcggctgt cgcggctggt
     1801 cctagcacag ggggcaggca gccgcgatgc aggcggcgtt cagcttcggt tcagcggacc
     1861 agtgcggcga gcagcgcgtc gagccgcagc ctgccttccg gggtcgcccg caagcgccct
     1921 gcctcccaca tcagataccc ggcttccagc gcatcgcgca gcgtgccggg gtcggtggcc
     1981 tgttcaagag cgagtccggt gcgggtgagg aagcgcgccg gatcgattcc ttcggtcaac
     2041 cgcaatccca tcagcagcat ttcccgcgcc cggtcgttcg cgtccagcag ggcgtcttcc
     2101 accgtgccgt gcccgacccg ctccacccgc tcggcccagg gttccggggc gcggtggcgg
     2161 cggacggcat acagcacgcc gtcgcgggtc agccggccat gcgcgccggg accgatgccg
     2221 gcgtaatcgg cgtagcgcca ataggccagg ttgtggcggc tttc
//
LOCUS       DASSZH010000377         3246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_809889, whole genome shotgun sequence.
ACCESSION   DASSZH010000377 DASSZH010000000
VERSION     DASSZH010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809889"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="VFN42_08245"
     CDS             <1..983
                     /locus_tag="VFN42_08245"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET8996644.1"
                     /translation="MKTAKSIDVARLALEDQPGGITVSTLAEAEYFAAHGISDILYAV
                     GVTPQKLDQVAKLNAAGASILVLTDDPDVASAIALHPLPPRTLIEIDTGESRGGVAPE
                     DPALLAIAQRLGHRLAGVMTHAGHSYAGRSRSDMVRIAEAERAGVVRAAERLRAAGHA
                     APIVSMGSSPTAMHAESLTGVTEVRAGVYMFGDLFQAEIGTHPTQDIAVTVLTSVIGR
                     RPGHILIDAGGLALSKDRSTEAAPKDYGFGLVLDLNGRPAFGDTVVRRAYQEHGLIEP
                     DAAHPIDLPVGGKLRIAPNHTCMTAAAHDRYHVVESSEDVVAVWPRINGW"
     gene            1124..1579
                     /locus_tag="VFN42_08250"
     CDS             1124..1579
                     /locus_tag="VFN42_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HET8996645.1"
                     /translation="MLALVGFVGLCLLVGAADAAIGSVGIRHWYPSLTPPPGTPPNWV
                     FGPVWSALYVLMGIAGWLVWRRCGASRPLRLWGWQLAANAVWTPFFFGLHNPVLALAD
                     MVVLIGLLGATIRGFHRVQPAAAGLLLPYLLWTFYAAYLNAGFWWLNRF"
     gene            1606..2247
                     /locus_tag="VFN42_08255"
     CDS             1606..2247
                     /locus_tag="VFN42_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007399663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET8996646.1"
                     /translation="MTRLAARVVVVVAGSLLLPQLALAQGAPLGAATGPGASGLIRPN
                     TQPQREIAPPAALPGAASQGTAAPATENPADMSPNAALFDAINRGDMAAARDALSRGA
                     ELNAHNVLGLTPIELAVDLGRHDIAFLLLSMRGADSSSGPPPAAAAAEALTAKPMPAA
                     AKPKPSQRIAARRAEAPAARPVPPEQPKLFANDGGSPIPSIGFLGFGNRPASP"
     gene            2347..2562
                     /locus_tag="VFN42_08260"
     CDS             2347..2562
                     /locus_tag="VFN42_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996647.1"
                     /translation="MPQDAKETARMMIRLHGLQAQAIAQERVSEMRQQGDTAGLDHWQ
                     HVHTAICELRQAGRAAEYGEHHADYRS"
     gene            complement(2619..>3246)
                     /locus_tag="VFN42_08265"
     CDS             complement(2619..>3246)
                     /locus_tag="VFN42_08265"
                     /inference="COORDINATES: protein motif:HMM:NF024820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET8996648.1"
                     /translation="SLAADVPNDSSMRSEIAERVAQAARSAGLPVPALPKGTTPEAAA
                     ANPDDAAMAAAAQMPEAQRKQFIQSMVAKLAAQMQSKPNDLGGWMRLGRAYAVLGDKA
                     KAVDAYQRAAALKPDDVTIKLREIEVLVTGLQPNDPLPKQVVGLLHEIQKVAPDQPEA
                     LWYLGVIAAREGHPLEARDDWNRLLKQLSPGSEDAKMVKAALDQVKLK"
BASE COUNT          474 a         1129 c         1101 g          542 t
ORIGIN      
        1 acatgaaaac ggcaaagtcg atcgacgttg cccgcctggc tttggaggac cagccaggcg
       61 ggatcaccgt ctccaccctg gccgaggccg agtatttcgc cgcccacggc atcagcgaca
      121 tcctctacgc ggtcggcgtc acgccgcaga agctggatca ggtcgccaag ctgaacgccg
      181 ccggcgccag catcctggtg ctgaccgacg atcccgacgt ggccagcgcc atcgcgctgc
      241 atccgctgcc gccccgcacg ctgatcgaaa tcgacacggg cgagtcgcgc ggcggtgtcg
      301 ccccggagga tccggccctg ctcgccatcg cccagcggct gggtcaccgg ctcgcggggg
      361 tgatgacgca tgccgggcac agctacgccg gccgcagccg gtccgacatg gtgcggatcg
      421 ccgaggcgga acgcgccggc gtggtgcggg ccgccgagcg gctgcgcgcc gccgggcatg
      481 cggcgccgat cgtgtcgatg ggcagctccc ccaccgcgat gcacgccgag tcgctgacag
      541 gcgtcaccga agtgcgcgcc ggggtctata tgttcggcga cctgtttcag gcggagatcg
      601 gcacccatcc gacgcaggat atcgccgtga ccgtgctgac cagcgtgatc ggccggcgcc
      661 ccggccacat cctgatcgat gccggcgggc tggcactgtc gaaggatcgc agcacggaag
      721 cggcgccaaa ggattacggg tttgggctgg tgctggatct gaatggccgc ccggccttcg
      781 gcgacacggt ggtgcgccgc gcctaccagg aacacgggct gatcgagccg gatgccgcgc
      841 acccgatcga cctgccggtc ggcggcaagc tgcggattgc tcccaaccac acctgcatga
      901 ctgccgcggc gcacgaccgc taccacgtgg tcgagagcag cgaggatgtc gttgcggtct
      961 ggccgcgaat taacggctgg taatagctct tccatcgcgc gcgggccggt cctacatccg
     1021 aacctgaacg ggccgtcacc gagcggccag gaggggtgtc ttccagcaga ctggtccgca
     1081 tgagtggggt tcgtcgcccg gttcgtccgc cccggtttcg ccggtgctgg cgctggtcgg
     1141 gttcgtcggg ctttgcctgc tggttggtgc cgccgatgcc gcgatcggca gcgtggggat
     1201 ccggcactgg tacccgtcac tgacgcctcc cccgggtaca ccgcccaact gggtattcgg
     1261 accggtctgg agcgctctgt acgtcttgat ggggatcgcc ggatggctgg tatggcgtcg
     1321 ctgcggcgcg tcgcggccgc tccggctgtg gggctggcaa ttggccgcca atgcggtttg
     1381 gacgccgttc ttcttcggct tgcacaatcc cgtcctggcg ctggccgaca tggtcgtgct
     1441 gatcggcctg ctcggcgcga ccatccgcgg cttccaccgt gtgcagccgg ctgccgctgg
     1501 gctgctgctg ccgtatctcc tgtggacgtt ttatgctgcc tacctcaacg ccgggttctg
     1561 gtggctgaac cggttctgag ctaatttgcc ggcatgcaaa gtgaaatgac gcgccttgcc
     1621 gcgcgagtcg tggttgtggt tgctggcagc ctgttgctgc cgcagcttgc cttggcccaa
     1681 ggcgcgccgc tcggcgccgc caccggtccc ggcgcctctg gcttgatccg cccgaatacc
     1741 cagccacaaa gggagatcgc gccgccggcc gcccttccgg gggccgcgtc ccagggcact
     1801 gccgctccgg ccaccgagaa tccggccgac atgtcgccca acgccgcgct gttcgacgcg
     1861 atcaaccggg gtgacatggc cgccgcgcgc gatgcgctga gccgcggcgc agagctgaac
     1921 gcccataacg tgctcggcct gacgccgatc gaactggcgg tcgatctggg acgccacgac
     1981 atcgccttcc tgctgctgtc catgcgcggc gccgacagtt ccagcggccc gccaccggct
     2041 gccgcggcgg cggaggccct tacggcgaaa ccgatgcccg cggcggcgaa gccaaagccg
     2101 tcacagcgga tcgcggcgcg ccgtgcggaa gcgccggcgg cgaggccggt gccgcccgag
     2161 caacccaagc tgttcgccaa tgacgggggc tcgcccattc ccagcatcgg gttcctggga
     2221 ttcggcaaca ggcccgcctc gccctgatcc tggatgaccg ggatatcatc gccggtcacg
     2281 accgttcgac tggcgatccg gcccgggcca tcacatatcc tgggggcttg aagcgaggag
     2341 aggatcatgc cccaggatgc gaaagaaacc gcccgcatga tgatccgtct gcacgggttg
     2401 caggcccagg cgattgccca ggagcgggtt tcggagatgc gccagcaggg agacaccgcc
     2461 ggcctcgatc actggcagca tgtccacacc gcgatttgcg agctgcgcca ggccggacgc
     2521 gccgcggaat acggcgagca tcacgcggac tatcgatcct gagcaacccg ccatgccctc
     2581 tccgcccatg tcgcggggcg gagagcggcg gcgcgtgatt acttcaactt cacctggtcc
     2641 agcgcagcct tcaccatctt ggcgtcttcg ctgccggggg aaagctgctt cagcaagcgg
     2701 ttccagtcat cccgggcctc gagcggatgg ccttcgcggg ctgcgatcac gccaagatac
     2761 cacagcgctt ccggctggtc cggggccact ttctggatct cgtgcagcag cccgaccacc
     2821 tgctttggca gcgggtcgtt cggctgcagc ccggtgacca gcacttcgat ttcccgcaac
     2881 ttgatggtga cgtcgtccgg cttcagcgcg gcggcgcgct ggtaggcatc gaccgccttg
     2941 gccttgtcgc cgagcaccgc ataggcccgg ccaagccgca tccagccgcc gaggtcgttc
     3001 ggcttgctct gcatctgggc ggcgagcttt gccaccatgc tctggatgaa ctgcttgcgc
     3061 tgggcttccg gcatctgcgc ggcggcagcc atggccgcgt catccggatt ggccgcggcc
     3121 gcctcgggcg tcgtcccctt cggcaatgcc ggaaccggca agccggcgga tcgggccgcc
     3181 tgggcgacgc gctccgcgat ttccgaccgc atggacgagt cgttcggaac gtcggcggcc
     3241 agactc
//
LOCUS       DASSZH010000378         4104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_588386, whole genome shotgun sequence.
ACCESSION   DASSZH010000378 DASSZH010000000
VERSION     DASSZH010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588386"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VFN42_08270"
     CDS             complement(<1..356)
                     /locus_tag="VFN42_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003622568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxI family protein"
                     /protein_id="HET8996649.1"
                     /translation="MPAQVARAARAAGRDVFIVGLDGFAERTVLAEWPHEIIRMGAAG
                     RILATLRAHGCQDLVLIGPVRRPSLLDMRPDAEGTRILARVGRAAFAGDDGLLAAVIR
                     VLGEEGFRVIGAQEVLR"
     gene            531..746
                     /locus_tag="VFN42_08275"
     CDS             531..746
                     /locus_tag="VFN42_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996650.1"
                     /translation="MTDDDEPSATDCHDAAIGILQGLHMLAEEADHLRLSRTYVALCK
                     AIRACQAEHARMTPGIRPRPRRYVTLH"
     gene            841..916
                     /locus_tag="VFN42_08280"
     tRNA            841..916
                     /locus_tag="VFN42_08280"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:874..876,aa:Asn,seq:gtt)
     gene            complement(968..1165)
                     /locus_tag="VFN42_08285"
     CDS             complement(968..1165)
                     /locus_tag="VFN42_08285"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HET8996651.1"
                     /translation="MLVQPRTNTERRKSRQAVTALEYALIVAAIALVIGSSTLVLGSH
                     LNRTFDQLWDLMRQSYSYRRG"
     gene            complement(1328..1540)
                     /locus_tag="VFN42_08290"
     CDS             complement(1328..1540)
                     /locus_tag="VFN42_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996652.1"
                     /translation="MKVRMRLALPLLLAVAGCAATPSPMTTSPGPVAQTSAAPPQRVT
                     AASEALGQRLDSMLANYRPAAMTLTR"
     gene            complement(1683..2831)
                     /locus_tag="VFN42_08295"
     CDS             complement(1683..2831)
                     /locus_tag="VFN42_08295"
                     /inference="COORDINATES: protein motif:HMM:NF011987.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HET8996653.1"
                     /translation="MDRLRIIGGRVLLPGGDAEVDLGIEDGRIAAIDGGGQGPALDAR
                     DLLVLPGIVDIHGDAFERQIQPRPGVDFPQEVGLADTEAQLLANGITTAFHGVTLSWE
                     PGLRSLDSWRALLDALEARRWTCDMRVHLRWEAYNLDALDTALADIAAGRVHLLAFND
                     HTPSILKKLDDPVAGAKYSDRAGMKLAEFRSLAERIAARAPEIGAAQARIAAAARAAG
                     LPLASHDDAEIPTRSHFRALGARICEFPMAEEVGEAARTADDWVVMGSPNVVRGRSHL
                     GWASAARLAEAGICNVLSSDYFYPAMHRAAWILSRRGVLDLARAWALISDNPAAAAGL
                     TDRGRIAPGLRADLVLMDSAAPRIVATVAAGRVAWLGAEGASRFIATN"
     gene            complement(2844..3602)
                     /gene="phnF"
                     /locus_tag="VFN42_08300"
     CDS             complement(2844..3602)
                     /gene="phnF"
                     /locus_tag="VFN42_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HET8996654.1"
                     /translation="MKLPGETDESVAHPVLRRDGVALWRQIVGYLQRDIDSGALPPGA
                     RLPTEAELSQQFRVNRHTVRRALEELSRGGVVRVEQGRGSFVAEDVLDYAVEPRTRFS
                     EWIRRHNKEPSGRVLQLKETTADSRIAAGLGLRSGARVVMLERLGLADDRPVSLTSHY
                     FAAARLPGLLEALRAAPRITEALKAVGVSDYMRQFTRVTARLPSPEEAELLRTGRSRP
                     LLVAENVNVDRDGAVLEFAVGLYPTPRVQIVFEP"
     gene            3706..>4104
                     /gene="phnG"
                     /locus_tag="VFN42_08305"
     CDS             3706..>4104
                     /gene="phnG"
                     /locus_tag="VFN42_08305"
                     /inference="COORDINATES: protein motif:HMM:NF018464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HET8996655.1"
                     /translation="MRTPAPQDDRRHWMAVLARASAAELQSALAAVGALPEYIRLRGP
                     EPGLVMVRGRIGGGGGAFNLGEMTVTRCAIRVAHGTVGHAYIAGRDERRAELAALCDA
                     LLQDEPDDGELHGTVARLHAAQQKCRATEAA"
BASE COUNT          674 a         1409 c         1377 g          644 t
ORIGIN      
        1 cgcagcacct cctgcgcacc gatgacccgg aagccctcct cgccgagcac cctgatcacc
       61 gccgccagca gcccgtcatc cccggcaaac gccgctcgcc cgacgcgcgc caggatgcgg
      121 gtgccctcgg cgtccgggcg catgtccagc agcgacggcc gccgcaccgg gccgatcagc
      181 accaggtcct ggcagccatg tgcccgcagc gttgccagga tgcggccggc ggcgcccatc
      241 cggatgatct catgcggcca ctccgccaac accgtccgtt cggcgaaccc gtcgaggcca
      301 acgatgaaca cgtcccgccc cgcagcacgt gccgcgcgcg ccacctgcgc cggcatggta
      361 ccgccgccgg caagaatgcc gagagtacgt ggcttttgcg ccgctaattc cgttgcagtt
      421 gcggcggagg gaaattcaag cattgcaaac tatccagcca ttcgacataa gaaccgcgcc
      481 ttcgctcacg acctcgtgag attgattacc gatagggatc aaaccgcctg atgaccgatg
      541 acgacgaacc gtctgccacg gattgccacg acgcggccat cgggattctt cagggtctcc
      601 acatgctggc cgaggaggcc gaccacctgc ggctttcgcg tacctatgtc gcactgtgca
      661 aggcgatccg tgcctgtcag gcggagcacg cccgcatgac ccccggcatt cggccgcgcc
      721 cgcgccgcta tgtgacgctg cactaggtgt cgagcgaagc acacgctcag atagcgaatt
      781 ttgtggcctg acggggtttg cgtcaggcag tcggcttcgc tataggaacg gccccgctga
      841 tcccggatag ctcagttggt agagcaagcg gctgttaacc gctgggtcgc aggttcgagt
      901 cctgctccgg gagccaatgg aaggggtcag gcctgacggc ctgacccctt tttgctgccc
      961 ggccgggtca cccgcgccgg tacgagtagg attgccgcat caggtcccag agctggtcaa
     1021 acgtgcgatt gagatgggat ccaagcacaa gcgttgaact gccgataacc aaggcaatgg
     1081 ccgccacgat caatgcgtat tccagtgcgg tgaccgcctg ccggctcttt ctccgctcgg
     1141 tgttcgtccg gggctggacc agcattctct atggttgttt ccctggattg gcatcgcgtc
     1201 cgatggataa gcgcgaacca tattttgcga ccggcgaaaa atactacgga acaggttacc
     1261 tcccgccttc cggcaacgaa aaggccgggg catgccccgg ccttccggtt cgccacaggc
     1321 agtgaactca gcgggtcagc gtcatggcgg cgggccggta gttcgccagc atgctgtcga
     1381 gccgctgtcc gagtgcctcg gaagctgccg tcacgcgctg cggcggggcc gcgctggtct
     1441 gcgcgaccgg cccggggctg gtggtcatgg gcgagggggt ggcggcgcag ccggcaacag
     1501 ccagcagcag cggcaaggcg agacgcatcc ggaccttcat tttatcgttc tccaacgggg
     1561 acgccgaggg gatcggcgcc ggatcgggtc tgttccgata ttttagacgc gagttactac
     1621 aaacagacta aatttcgcgc ggtgatccgt gtcccagccg aggattgcgt gtcaaccctg
     1681 tatcaattcg tcgcgataaa gcgggacgcc ccttccgctc ccagccaagc cacccggccg
     1741 gccgcgaccg tggcgacgat acgcggtgcg gcggagtcca tcagaaccaa gtcggcgcgg
     1801 aggcccggcg cgatccggcc ccgatcggtc aatccggccg cggcggcggg attgtccgaa
     1861 atgagcgccc aggcccgggc caggtccagc acgccccgcc gggacaggat ccaggcggcg
     1921 cggtgcatgg ccggatagaa gtagtccgac gacagcacgt tgcagatacc cgcttcggcg
     1981 agccgcgcgg cggaggccca gccgaggtgc gagcggccgc gcaccacgtt gggactgccc
     2041 atcaccaccc agtcgtccgc ggtgcgggcg gcttcgccga cctcctccgc catggggaac
     2101 tcgcaaatcc gggcgccgag cgcgcggaag tggctacggg tgggtatttc ggcgtcgtca
     2161 tggctggcca gcggcaggcc cgcagcgcgc gcggcggcgg cgatgcgcgc ctgtgccgca
     2221 ccgatttccg gcgcgcgggc ggcgatgcgc tctgccaggg agcggaattc ggccagtttc
     2281 atgccggccc gatcgctgta tttcgcgccg gcgaccggat cgtccagctt cttcaggatc
     2341 gacggggtat ggtcgttgaa cgccagaagg tgcactcgcc cggcggcgat atccgccagg
     2401 gcggtgtcca acgcatccag gttgtaggcc tcccagcgca ggtgcacgcg catgtcgcag
     2461 gtccagcgcc gagcttccag cgcatccagc agcgcacgcc aggagtccag gctgcgcagg
     2521 cccggttccc aggacagcgt cacgccatgg aacgccgtgg tgatgccgtt ggcgagcaac
     2581 tgcgcttcgg tatcggcgag cccgacctcc tgcgggaaat cgacgccggg ccgcggctgg
     2641 atctgccgct cgaacgcgtc gccatgaata tcgacgatac cgggcagcac cagcaggtca
     2701 cgcgcgtcca gtgccggtcc ctgcccgccg ccgtctatcg cggcgatgcg gccgtcctcg
     2761 atgccgagat ccacctcggc gtcaccgccg ggcagcagca cgcgtccgcc gatgatgcgc
     2821 agcctgtcca tgctgtccga ttcctatggt tcgaagacga tctgcacgcg cggcgtggga
     2881 tacagcccga ccgcgaattc cagcaccgcg ccatcgcggt cgacattgac gttctccgcc
     2941 accagcagtg gccggctgcg gccggtgcgc agcagctccg cttcttccgg gctgggcagc
     3001 cgtgcagtga cccgggtgaa ctggcgcatg tagtcggaca ctccgaccgc cttcagcgcc
     3061 tcggtgatgc gcggcgcggc gcgcaacgcc tccagcaatc cgggcaggcg cgcggcggcg
     3121 aaatagtggc tggtcaggct gaccgggcga tcgtcggcca ggcccagccg ctccagcatc
     3181 acaacgcggg caccgctgcg caggccaagc ccggcggcga ttcgcgaatc ggccgtggtc
     3241 tccttcaatt gcagcacgcg cccggacggc tccttgttgt gccggcggat ccattcggag
     3301 aaccgggtgc gcggttcgac ggcgtagtcc agcacgtcct cggccacaaa cgatccacgc
     3361 ccctgctcca cccgtaccac cccgccgcgc gacagctcct ccagcgcgcg ccggacggtg
     3421 tggcggttga cccggaactg ctgggacagt tcggcctcgg tgggtagccg ggcgccgggg
     3481 ggaagtgcgc cgctgtcgat atcccgctgc aggtagccga cgatctgccg ccatagcgcc
     3541 acaccatcac ggcgcagcac cggatgggcg acagactcgt ccgtctcgcc tggcagcttc
     3601 atggttattt catccaaacc ggcggcgtgc cgggcaagtt cggcattgac gctagcctgg
     3661 ggcgcggata gctgtctaga cgtctaaatg aatttgggag cggccatgcg gaccccagcg
     3721 ccccaggacg acaggcgcca ctggatggcg gtgctggcac gggccagcgc ggcggagctg
     3781 cagtcggcac tggccgcggt tggtgcgcta cccgagtaca tccggctgcg cggcccggag
     3841 cccgggctgg tcatggtgcg cggccggatc ggcggcggtg gcggcgcctt caacctgggc
     3901 gagatgacgg tgacccgctg cgcgatccgc gtcgcgcacg gcaccgtcgg ccatgcctat
     3961 attgccggcc gcgacgaacg ccgggcggaa ctggcggcgc tgtgcgatgc cctgctgcag
     4021 gacgaaccgg acgatggcga gctgcacggc accgtcgcac ggctgcacgc cgcgcagcag
     4081 aagtgccgcg ctaccgaggc tgcc
//
LOCUS       DASSZH010000379         2509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1543011, whole genome shotgun sequence.
ACCESSION   DASSZH010000379 DASSZH010000000
VERSION     DASSZH010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543011"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VFN42_08310"
     CDS             <1..771
                     /locus_tag="VFN42_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019090535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8996656.1"
                     /translation="GEVRRGPKLKVGYFAQHQTDELIAGESPINHMARALPRATPPQV
                     RSQLARFGLDADRAETPVDKLSGGEKARLLLALATRDAPQLLILDEPTNHLDIDAREA
                     LVKALADFQGAVLLITHDPHLVELVADRLWLVADGTVRSFDGDLDDYRALLAQRARPA
                     DTGGTTARRDERRERAEARAALAPLRKQAKEAEARLARLTAERGKLEAKLADTSLYTS
                     GRTQDITGANTRLAAIAREMAEAEAAWLAAEEALEAAN"
     gene            850..1416
                     /locus_tag="VFN42_08315"
     CDS             850..1416
                     /locus_tag="VFN42_08315"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET8996657.1"
                     /translation="MRPSALTHEPETMMTREQYRAWAEQQPQNRFERHHGIVIAMAPE
                     RAGHALRKAAARDELLRGVRAASLPCQVFPDGMTIEIDDSDYEPDAVLRCGPRLPDDA
                     IAIPDPLVIVEVLSPGTSAIDRGHKLRAYLRVASLRHYLIVWPDTPRVVHHHLAEGGK
                     LATEVFTEGEIQLDPPGIRLEVAALYAD"
     gene            complement(1436..2104)
                     /locus_tag="VFN42_08320"
     CDS             complement(1436..2104)
                     /locus_tag="VFN42_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HET8996658.1"
                     /translation="MNVLAPPKDRLGIEIVHDLVCPWCFLGVRRLMRTLRRRPDLLFE
                     ITWRPFLLNPDMPRLGMARPDYVIRKFGGEDRARRLYASISEIGRNEGVAFRFDRIRR
                     TPSSVDAHRLVRYADRYGRADAMVEALFSAHFTDGLDIGEVSVLIAVAQSCGLNAPSV
                     RRFLLSDEEVDAVHGDNLRAHRLGINGVPCFVISGRHAIAGAQEPEVIERLLDVAAVE
                     AAER"
     gene            complement(2197..>2509)
                     /locus_tag="VFN42_08325"
     CDS             complement(2197..>2509)
                     /locus_tag="VFN42_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996659.1"
                     /translation="ICVFPGSHRLPVPAGQAVEPLMAEQAIQPAPIGGKRGRVVLRHP
                     ALVHKAEPVGPAAIRRTLLAQYCPVHVAPLYAERVATAVWPHGSHWFTTQHYAGIEPL
                     D"
BASE COUNT          423 a          866 c          881 g          339 t
ORIGIN      
        1 ggcgaggtgc ggcgcgggcc gaagctgaag gtggggtatt tcgcccagca tcagaccgac
       61 gagctgatcg ccggtgaaag cccgatcaac cacatggccc gcgccctgcc gcgcgccacg
      121 ccgccgcagg tgcgctcgca actggcgcgg ttcgggctgg acgccgaccg ggcggagacg
      181 ccggtggaca agctgtcggg cggcgagaag gctcgcctgc tgctggcgct ggccacccgc
      241 gacgcgccgc agctgctgat cctggacgag cccaccaacc atctggacat cgacgcgcgc
      301 gaggcgctgg tgaaggcgct ggccgacttc cagggcgcgg tgctgctgat cacccacgac
      361 ccgcatctgg tggagctggt ggccgaccgg ctgtggctgg tggccgatgg cacggtgcgg
      421 tcgttcgatg gcgacctgga cgactaccgc gcgctgctgg cccagcgcgc ccgcccggcc
      481 gacaccggtg gcaccaccgc ccgccgcgat gaacgccgcg aacgcgccga ggcccgcgca
      541 gcgctggccc cgctgcgcaa gcaggcgaag gaggccgaag cccggctcgc ccgcctcacc
      601 gccgaacgcg gcaagcttga ggcgaagctg gcggatacgt cgctgtacac ctccggccgg
      661 acccaggaca tcaccggcgc caacacaagg ctggcggcga tcgcccggga aatggccgag
      721 gccgaggccg cctggctggc ggcggaggag gcactggaag ccgccaactg agcgccgcgg
      781 cgcggatgcg cttgaccgcc cgccggggcc gcaccgatat gatcacagcc gacctgccag
      841 gaggccgcca tgcgtccatc cgcgctgacg cacgaaccgg aaaccatgat gacccgggag
      901 cagtatcgcg cctgggcaga acaacagccg cagaatcgct tcgagcggca tcatggcatc
      961 gtgattgcca tggcgcccga gcgggccggg cacgcgctgc gcaaggccgc cgcccgggat
     1021 gaattgctgc gcggcgtgcg cgccgccagc ctgccttgcc aagtatttcc tgacggcatg
     1081 acgattgaaa tcgacgacag cgactacgag ccggatgcgg tactgcgttg cgggccgcgc
     1141 ctgccggacg atgccattgc cattcccgac ccgctggtga tcgtggaggt gctgtcgccc
     1201 ggcaccagcg caatcgaccg cggccacaag ctgcgggcgt atctccgggt cgcctcgctc
     1261 cggcattacc tgatcgtgtg gccggatacg ccgcgggtcg tgcatcatca cttggccgaa
     1321 ggcggcaagc tggccaccga ggtcttcacc gaaggggaaa tccagctcga tccacccggg
     1381 atccggctgg aggttgcagc gctttacgcg gattgatgcg gcagggccgc gtggctcacc
     1441 gctccgccgc ctcgaccgcg gcgacgtcca gcagccgctc gatcacctcg ggctcctggg
     1501 cgccggcaat cgcgtgccgg ccggagatga cgaagcacgg gacgccgttg atgcccagcc
     1561 ggtgggcgcg caggttgtcg ccgtgcaccg cgtccacctc ctcatcgctg agcaggaagc
     1621 ggcggaccga cggcgcgttg agcccgcagg attgcgcgac cgcgatcagc accgagactt
     1681 cgccgatatc cagcccatcg gtgaaatgcg ccgagaacag cgcctcgacc atcgcatcag
     1741 cccggccgta gcggtcggcg tagcgcacca gccggtgggc atccaccgag gacggggtgc
     1801 ggcggatacg gtcgaagcgg aaggcaacac cctcattgcg gccgatctcg gaaatcgagg
     1861 catacagccg ccgcgcccgg tcctccccgc cgaatttgcg gatcacgtag tccgggcggg
     1921 ccatgccgag gcgcggcata tcggggttga gcaggaaggg ccgccaggtg atttcgaaca
     1981 acaggtccgg gcggcggcgc agcgtgcgca tcaggcggcg cacgcccagg aaacaccaag
     2041 ggcagacgag atcgtgcacg atctcgatcc cgagccggtc ttttggtggt gcaaggacgt
     2101 tcaccagggg attctggcgc gccgagcccg gcttgtcacg cgaatatcat acgatgcccg
     2161 gatgcgtacg ctccggcagc accgaggggt cacacatcaa tccagcggtt caatccctgc
     2221 atagtgctgc gtggtgaacc agtgagaccc gtgcggccag accgccgtgg cgacgcgttc
     2281 ggcgtagagc ggcgccacat ggacggggca atactgtgcc agcagcgtgc ggcggattgc
     2341 cgcgggtccg accggttccg ctttgtgcac cagcgccgga tggcgcagca ccacccggcc
     2401 gcgcttgccg ccgatcggcg cgggctggat cgcctgctct gccatcagcg gttccactgc
     2461 ctgcccggcg ggaaccggca agcggtggct gcctgggaac acgcaaatc
//
LOCUS       DASSZH010000380         2290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_486156, whole genome shotgun sequence.
ACCESSION   DASSZH010000380 DASSZH010000000
VERSION     DASSZH010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486156"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VFN42_08330"
     CDS             <1..332
                     /locus_tag="VFN42_08330"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET8996660.1"
                     /translation="NPKIARSQCVGGMVGGLGMALLEQAEWDPHLGRVMNANLAEYLV
                     PVNADVQQLEAVFVQGEDTIFNPIGSKGVAEIALVGVAPAIASAVYHATGKRVRNLPI
                     TPEKLLG"
     gene            complement(476..664)
                     /locus_tag="VFN42_08335"
     CDS             complement(476..664)
                     /locus_tag="VFN42_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996661.1"
                     /translation="MRHDAKQGTEAAQHAAQEPPEVSPLLVGPRSELGLLRLEQPLKP
                     GKRRRIRLDDLLQQGDTG"
     gene            718..1467
                     /gene="recO"
                     /locus_tag="VFN42_08340"
     CDS             718..1467
                     /gene="recO"
                     /locus_tag="VFN42_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET8996662.1"
                     /translation="MDWEAPAIVLDARPYGESGAIVTVMTEAHGVHRGLARGGTSRAQ
                     APLWQPGNLAQIRWVARLADQLGSFSGELVHAGAAQVLDEPLPLAMLAAACAVAEGAL
                     PEREPHPIVFSGLLRLIAHLPQGAPLLPDLVRWELALLAELGYGLDLSRCAVSGETAG
                     LAFVSPKSGRAVTEAAAGAWKDRLLRLPGFLAGGNIAAPADWRDGLALTAHFLARDAF
                     GHQHRPLPLPRQMLYDRVAALAAESEDGDAG"
     gene            1457..>2290
                     /locus_tag="VFN42_08345"
     CDS             1457..>2290
                     /locus_tag="VFN42_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010513493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET8996663.1"
                     /translation="MPDEAGHIEDTQLKDALSERYLAYALSTIMSRSLPDVRDGLKPV
                     HRRLIHAMNLLRLDPNAGFKKCARVVGDVIGRFHPHGDVAVYDALVRLAQDFAARYPL
                     VEGQGNFGNVDGDNPAAMRYTEARLTEVAKVMLAGIDQDAVDFRPNYDGEESEPVVLP
                     GAFPNLLANGAAGIAVGMATSIPPHNAGEVCAAAIHLIQHPNATTADLLRHMPGPDFP
                     TGGVLVEDPAAILTAYETGRGGFRVRAKWQREDGKHGTWQIVVTEIPYQVQKSKLIEH
                     VA"
BASE COUNT          354 a          802 c          797 g          337 t
ORIGIN      
        1 tgaacccgaa gatcgcacgc agtcagtgcg tcggtggcat ggttggtggc ctcgggatgg
       61 cgctgctgga acaggcggaa tgggatccgc acctgggacg cgtgatgaac gccaacctgg
      121 cggagtacct ggtgccggtg aatgccgacg tgcagcagtt ggaggcggtg ttcgtgcaag
      181 gcgaagacac gatcttcaac ccgatcggct ccaagggcgt ggcggagatc gcgctggtgg
      241 gggtggcgcc ggcgatcgcc agtgcagtct atcacgccac cggcaagcgg gtgcgcaacc
      301 tgccgatcac gccggagaag ctgctggggt gaggcctggt cgggcggcag atcgtccgcc
      361 tggtcaagct tttcttcgcg ccgatgggcg agactggcgc cgcggcagac cgagtcacgc
      421 ggtgccagac cttcccgttg aacccgccgg cggcggccgg tatcctgacg acatgtcaac
      481 ccgtgtcgcc ctgctggagc agatcgtcca gacgaatgcg gcgacgtttg cccggtttga
      541 gcggctgttc gaggcgcaac aagccgagct ccgagcgcgg tccgaccagc agcgggctga
      601 cttccggtgg ctcctgggca gcatgctggg cggcttcagt gccctgcttg gcgtcatggc
      661 gcacgggctc cactggttgt agcctgtccg gccccgccac aacccaaacg actgagcatg
      721 gactgggaag ccccggcgat cgtgctggac gctcgcccat acggcgagtc cggtgccatc
      781 gtcaccgtca tgaccgaagc gcacggcgtg caccgcggcc tcgcccgcgg cggcacctcg
      841 cgcgcacagg cgccgctgtg gcagccgggc aatctggcgc aaatccgctg ggtggcgcgg
      901 ttggccgacc agctgggcag cttcagcggc gaactcgtgc acgccggcgc ggcgcaggtg
      961 ctggatgagc ccctgccgct ggccatgctc gctgccgcct gcgccgtggc ggaaggcgcg
     1021 ctgccggaac gggaaccgca ccccatcgtc ttctccggcc tgctgcgcct gatcgcccac
     1081 ctgccgcaag gcgcgccgct gctccccgac ctggtgcgct gggaactggc cctgctggcg
     1141 gagctcggct acggcctgga cctgtcccgc tgcgccgtca gcggggaaac cgcgggcctg
     1201 gccttcgtct cacccaaatc gggccgagcg gtcaccgaag cggcggcggg ggcgtggaag
     1261 gaccggctgc tgcggctgcc gggcttcctg gccggcggca acatcgccgc cccggcggac
     1321 tggcgcgacg ggctggcgct gaccgcccac ttcctggcgc gggacgcctt cggccaccag
     1381 caccggccgc tgcccctgcc gcgccagatg ctatacgacc gcgtcgccgc gctggcggcc
     1441 gaatcggagg acggcgatgc cggatgaagc aggacatatc gaggacacgc agctgaagga
     1501 tgcgctgtcg gaacgctacc tggcgtacgc gctgtccacc atcatgtcgc gcagcctgcc
     1561 ggatgtgcgc gacgggctga agccggtgca tcgccggctg atccacgcga tgaacctgct
     1621 gcgcctcgac cccaatgccg gcttcaagaa atgcgcccgc gtcgtcggcg acgtgatcgg
     1681 ccgcttccac ccgcatggcg acgtggcggt gtacgacgcg ctggtgcgcc tcgcccagga
     1741 tttcgccgcc cgctacccgc tggtggaagg gcaggggaat ttcggcaacg tcgatggcga
     1801 taatcccgcg gcgatgcgct acaccgaggc gcggctgacc gaggtcgcca aggtgatgct
     1861 ggcgggcatc gaccaggacg cggtggattt ccgccccaat tacgacggcg aggaatccga
     1921 acccgtcgtg ctgcccggcg cgttccccaa cctgctggcg aacggcgccg ccggcatcgc
     1981 ggtgggcatg gcgacctcca tcccgccgca caacgcgggc gaagtctgcg ccgccgcgat
     2041 ccacctgatc cagcatccga acgccaccac cgccgacctg ctgcgccaca tgcccggccc
     2101 cgatttcccc accggcggcg tgctggtgga agatcccgcc gccatcctca ccgcctatga
     2161 aaccggccgc ggcggcttcc gcgtgcgcgc caagtggcag cgcgaggacg gcaagcacgg
     2221 cacctggcag atcgtcgtca ccgaaatccc ctaccaggtg cagaaatcca agctgatcga
     2281 gcacgtcgcg
//
LOCUS       DASSZH010000381         2021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1526217, whole genome shotgun sequence.
ACCESSION   DASSZH010000381 DASSZH010000000
VERSION     DASSZH010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526217"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1293)
                     /locus_tag="VFN42_08350"
     CDS             complement(<1..1293)
                     /locus_tag="VFN42_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8996664.1"
                     /translation="MTNAVPFDRVVRGTVVTADTVLPDGYVAIRGETIAAIGRGTPPP
                     ATETNDYGDRLILPGLVDGHMHTSSSTGWPGIESATRCAAAGGVTTCCDMPYDVPQPV
                     TDAAILADKIGWVQRTSHVDMALYGTIQKQGGLDAIAELAADGISAFKLSTYEYDAVR
                     FPRIDHPTMLAAFREIARTGLPVAIHNEDQELVETLTVQARAAGRIEPIMHCRTRPPL
                     AESLADLEIFEMALETGAHVHIAHSLIARGFELAATFRGMGAQTTGEACIQYLCMTED
                     DIIRLAGIGKCNPPFRSAAEVERMWTALLADKVAYVSTDHAPWPIERKTLPDIFACGA
                     GLTGLQSFAPLMFSLLEERGLPPTLMATYCAERSAKLHGLWPRKGAIRVGSDADLLVL
                     ERRDFVFDQAALLDRPEMCWSPYHGRRMRGRVAATWLRG"
     gene            1557..>2021
                     /locus_tag="VFN42_08355"
     CDS             1557..>2021
                     /locus_tag="VFN42_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019088485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HET8996665.1"
                     /translation="MILHDVRVHPSKDNLPREEQLAWKIAAVAADKVAVERDVTEMII
                     NRIIDNASVAIASVNRQPIISARDAAIGHPRKDGATIFGMPHSKGFSPEWAAWANGTA
                     VRELDMHDTFLAADYSHPGDNIPPVLAVAQSMEKSGRDLIRGIATGYEIHIDL"
BASE COUNT          333 a          704 c          670 g          314 t
ORIGIN      
        1 cccgcgcagc caggtcgcgg ccacgcggcc ccgcatccgg cgcccgtgat agggcgacca
       61 gcacatctcc ggccggtcca gcaatgccgc ctggtcgaac acgaaatcgc ggcgctccag
      121 caccagcagg tcggcgtcgg agcccacgcg gatcgcgccc ttccgcggcc atagcccgtg
      181 cagcttcgcg ctgcgctcgg cgcagtaggt cgccatcagc gtcggcggca ggccacgctc
      241 ctccagcagc gagaacatca gcggcgcgaa actctgcaat cccgtcagcc cggcaccgca
      301 ggcgaagatg tccggcagcg tcttgcgttc gattggccag ggcgcgtggt cggtggagac
      361 atacgcgacc ttgtcggcca gcagcgccgt ccacatgcgc tccacctccg ccgcgctgcg
      421 gaagggtggg ttgcatttgc cgatgccggc caggcggatg atgtcgtcct cggtcatgca
      481 cagatattgg atgcacgcct cgccggtggt ctgcgcgccc atgccgcgga aggtcgccgc
      541 cagttcgaag ccgcgcgcga tcaaggagtg cgcgatgtgc acgtgggcgc cggtctccag
      601 cgccatctcg aagatctcca ggtccgccag gctttccgcc agcggcggac gggtgcggca
      661 atgcatgatc ggctcgatcc ggccggccgc gcgtgcctgc acggtgagcg tctccaccag
      721 ctcctggtcc tcgttgtgga tcgccaccgg caggccggtc ctggcgatct cgcggaatgc
      781 cgccagcatc gtcgggtgat cgatgcgggg gaaacgcacg gcgtcgtact cgtatgtaga
      841 gagcttgaac gcggagatgc cgtcggccgc cagttcggca atggcatcca gcccgccttg
      901 cttctggatc gtgccgtaca gcgccatgtc cacatgcgac gtgcgctgca cccagccgat
      961 cttgtccgcc aggatcgccg catcggtcac cggctgcggc acgtcgtacg gcatgtcgca
     1021 gcaggtagtg accccgcccg cggcggcaca gcgcgtcgcg ctttcgatgc ccggccaacc
     1081 ggtggaggag gaggtgtgca tgtgcccgtc aaccagtccg ggcaggatca gcctgtcgcc
     1141 gtaatcgttc gtctccgtgg cgggcggtgg cgttccccgg ccgatggccg cgatggtctc
     1201 gccgcggatc gcgacatagc cgtcaggcag cacggtgtcg gcggtcacca cggtgccgcg
     1261 gacgacccga tcgaacggaa cggcgttggt catgcgagcg gtgctccatt gctgcggcat
     1321 gccttcggag aaggcggccc atgctagccg aagcgatgac cgagcggaaa atccatgggt
     1381 ccgaacgccg gcaggcgcga ccggcgtggc gcgacacctc acttatatat ctgtacgccg
     1441 cggccttcgc ccctccgctt gccgttacgg cggcacactc ttacgaagat cggagacggc
     1501 caggaccgcg cggtttcggc agcccctctg ccgctccgcg cccagcggag gaagacatga
     1561 tactgcacga cgtcagggtt cacccatcga aggacaatct gccgcgcgag gagcagctcg
     1621 cctggaagat cgccgccgtc gccgccgaca aggtggcggt ggagcgcgac gtcaccgaga
     1681 tgatcatcaa ccgcatcatc gacaatgcgt cggtggcgat cgcctcggtg aaccgccaac
     1741 cgatcatttc cgcgcgcgat gccgcgatcg gccatccgcg caaggacggc gccacgatct
     1801 tcggcatgcc gcacagcaag gggttcagtc cggaatgggc cgcctgggcg aacggcaccg
     1861 cggtgcgcga gctggacatg cacgacacct tcctcgccgc cgactactcg catcccggcg
     1921 acaacatccc gccggtgctc gccgtggcgc agagcatgga gaagtccggc cgcgacctga
     1981 tccgcggcat cgccaccggc tatgaaatcc atatcgacct g
//
LOCUS       DASSZH010000382         5682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_929347, whole genome shotgun sequence.
ACCESSION   DASSZH010000382 DASSZH010000000
VERSION     DASSZH010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5682
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929347"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VFN42_08360"
     CDS             <1..671
                     /locus_tag="VFN42_08360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8996666.1"
                     /translation="FTYDGKFWKVQKPEPMFEFLKPHIYPLQKPHPPIGVAGLSKQSD
                     TLKLAGERGFLPLSLNLNPAYVASHWEAVEIGAARTGRTPNRADWRLVREVFVADTDA
                     EAWRLCVDGMMGRMMREYFLPLLGNFGFLEYLKHDPGVADSDVTPEYCAKHNWLVGSP
                     ATVAEKLERIYHEAGGFGGLLVFGFDYLENPQAWHRSLKLFKEEVAPRVAHLIPDGKK
                     AAAE"
     gene            907..1563
                     /gene="tagF"
                     /locus_tag="VFN42_08365"
     CDS             907..1563
                     /gene="tagF"
                     /locus_tag="VFN42_08365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagF"
                     /protein_id="HET8996667.1"
                     /translation="MAGLNPRPPGVMVADPGIVAGFYGKLPARGDFVRFALPGAFVDS
                     WDTWLQRVIVGSRAQMGENWLPAYLEAPVWRFALPPACCGASSVLGLMLPSVDRAGRY
                     FPLTFAALAPIGPDIARQPRATEWLDRCEAAGRTALADDVTPDQLGAMLDLPTWPPGA
                     TTLSQSLWWTDGAPRVPPASFTQEGMPDPATFAHMLDAQQAGPSATNTGARVAWEQPS
                     "
     gene            1560..2285
                     /locus_tag="VFN42_08370"
     CDS             1560..2285
                     /locus_tag="VFN42_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HET8996668.1"
                     /translation="MTEIRLRSWATTHPGAKRKHNEDAYINRPDIGVWAVADGAGGHQ
                     AGEVASGMIAEALASIPPGLSGAELLAHVRTRIEETHLALRDLAEQRGPNVTVASTVV
                     VLLARDEHFACLWAGDSRAYLLRNGELRQISHDHSLVQELLDSGAITPEEAETHPRAN
                     VITRAVGADVDEFVLDKVSGRLMQGDRFLLCSDGLCKTVPEWQLTQVLGAEDGDSPTD
                     GLIAAALALNANDNVTAVTVEAD"
     gene            complement(2373..3911)
                     /locus_tag="VFN42_08375"
     CDS             complement(2373..3911)
                     /locus_tag="VFN42_08375"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET8996669.1"
                     /translation="MQNLTDAPPARVQPAPPAPVRDGATGRYLLWLILATTAIRLAFA
                     ASTGLGVDESYMISAGRVLSLGYFDHPPAAWWLSWGAAHLFGSEAPVVVRLPFIALFA
                     LSTWLMARLGAAVAGPRAGLWAAVLVNLSPVFGITTATWVLPDGPLDCALLGAALCML
                     HALPAGGRAAWGWWATAGLCAGLAMFSKYSAGLTVLGAGLYLLASAPHRHWLARPQPY
                     LAGVVALLVFSPVLVWNATHHWASFAFQGDRAEGLRFHPFASFGVLAGEALFVLPWIW
                     APMMVAWIAALRRGSADWRRWLLCWLGMPPIVLFAAIAVWSSQRVLFHWAAPGYLMLF
                     PLLGEAVALRIGHPVVRRILIGTAALAVGALVVIGTQLSLGWLEPVVMAFARHDPTVE
                     GIDWSSVRADLAARGLLHSGTVVGVPNWRDAGKIAYALGPEVTVLCLNRDSRQFGFAH
                     PAAAYLGHDVLVLAPEHRERVPAELGADFRALRRLPDAPITHAGDVLQWVGVYQGDDL
                     RAAP"
     gene            complement(3985..>5682)
                     /locus_tag="VFN42_08380"
     CDS             complement(3985..>5682)
                     /locus_tag="VFN42_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HET8996670.1"
                     /translation="TKLVVNGAGAAGIACVELLKSMGMRADNVILCDTKGVIYEGRTE
                     GMNQWKSAHAAPTQARTLAEALDGADVFFGLSQKGAVDQQAVRQMADRPVIFAMANPD
                     PEITPEEAREASPHAIIATGRSDYPNQVNNVLGFPYIFRGALDVRASTINDAMKIAAA
                     EALAKLAREDVPDEVHSASTGRRLSFGPEYIIPNAFDPRLISTVPPAVARAAMDSGVA
                     RRPISDLRRYARELSSRLDPTAAALDAIMESVSDNPRRVAFAEGEEEKVVRAAVAFRN
                     AGYGTPVLIGREDRVQSTMNSLGLGMVEGIEIHNARLSSANSKYVEFLYRRLQRKGYL
                     VRDCQRMVNQDRNVFAACMVATGDADAMVTGLTRSAAVCLDDIGKAIGPMPGGMAFGL
                     TLLVGMRGRTIFVADTAMNFRPNAEQLADIAVGAAAAARHLGHEPRVALLSHSVFGDP
                     PNERAIPMRDAVAVLDQRKVDFEYDGEMSPDVALEPSIRALYPFCRLTDNANVLIMPG
                     LHSALITSRLVHRLGGGTTIGPLLIGMEKPVQVVPMDASVSQIVDMACLAAYQAAER"
BASE COUNT          964 a         1973 c         1903 g          842 t
ORIGIN      
        1 gcttcaccta cgacggcaag ttctggaagg tgcagaagcc ggaaccgatg ttcgagttcc
       61 tcaagcccca catctatccg ctgcagaagc cgcatccgcc gatcggcgtg gcaggactgt
      121 cgaagcagtc cgacacgctg aaactggccg gtgaacgcgg cttcctgccg ctatcgctga
      181 atctgaatcc ggcttacgtc gccagccatt gggaagcggt tgagatcggc gccgcgcgca
      241 ctggccgcac gccgaaccgg gcggattggc ggctggtgcg cgaggtattc gtcgccgaca
      301 ccgatgccga ggcctggaga ctctgcgtcg atggcatgat gggccgcatg atgcgcgagt
      361 acttcctgcc gctgctcggc aatttcggtt tcctggagta cctgaagcac gatccgggcg
      421 tggcggattc cgacgtgacg ccggaatact gcgcgaagca caactggctg gtcggctcgc
      481 cggcaaccgt cgcggagaag ctggagcgca tctaccacga ggccggcggg ttcggcggcc
      541 tgctggtgtt cggcttcgac tacctggaga atccccaggc ttggcaccgc tccctgaagc
      601 tgttcaagga agaggtggca ccgcgcgtcg cgcacctgat tcctgacggc aagaaggcgg
      661 ccgcggaata gcatcacgcc gatcgccatg gccggtgcag gctggccatg gcgcggattg
      721 ccgccgcgtg ctcgccggcg attcgtccaa tcgcgcttga ccggcgcggc ccgcccccgc
      781 catgacgtgc ccgttccgcg caacgcgatt gttgcaggct gcggtgcggc tgcgcgagga
      841 tggcaggcag ctttgaggga tggaaacaat gggccggacc agtccagcgg tctgtcggcg
      901 cctgggatgg caggcctcaa cccgcgcccg ccaggcgtga tggtcgccga tccaggcatc
      961 gtggccgggt tttatggcaa gctcccggcg cgcggcgact tcgtgcgatt cgcgctgccc
     1021 ggtgccttcg tcgactcctg ggatacgtgg ctgcagcggg tcatcgtcgg cagccgcgca
     1081 cagatgggtg aaaactggct tcccgcctat ctggaggccc cggtctggcg cttcgccctg
     1141 ccgcccgcat gttgcggcgc gagcagcgtg ctgggcctga tgctgcccag cgtggatcgc
     1201 gccgggcgtt actttccgct gaccttcgcc gcgctcgccc cgatcgggcc ggacattgcc
     1261 cggcaaccac gcgccacaga gtggctcgac cgctgcgagg cggccggacg caccgccctg
     1321 gcggacgatg tcacgccgga tcagctcggc gccatgctcg accttccaac ttggccgcca
     1381 ggtgcaacca ctctgtcgca atcgctgtgg tggactgacg gcgccccgcg tgtgccgcca
     1441 gccagcttca cacaagaggg catgccggac cctgcgacct tcgcacacat gctggatgcg
     1501 cagcaggccg gaccgagtgc gacaaacaca ggggcacgcg tcgcatggga acagccgtca
     1561 tgaccgagat tcgcctccgc tcgtgggcaa ccacgcatcc gggtgcgaag cggaagcata
     1621 acgaggacgc gtatatcaac cgtccggata tcggcgtctg ggcagtggcc gacggggccg
     1681 gtggccacca ggccggcgaa gtcgcctccg gcatgatcgc agaggcattg gcgagcattc
     1741 cacccggcct gtcgggcgcg gaactgctgg cgcatgtgcg cacacggatc gaggaaaccc
     1801 acctcgcctt gcgtgatctg gcggagcaac gcggacccaa cgtgacggtg gcctccaccg
     1861 tcgtggtgtt gctggcgcgc gacgagcact ttgcctgcct gtgggcgggc gattcccgtg
     1921 cctacctgct gcgcaacggc gagctgcggc agatcagcca cgaccatagt ctggtgcagg
     1981 agctgctgga ttccggcgcg atcacgccgg aggaggcgga gacgcatccg cgcgccaacg
     2041 tgatcacccg cgcggtcggc gcagatgtgg acgaattcgt gctggacaag gtcagcggcc
     2101 ggttgatgca gggcgaccgc ttcctgctgt gcagcgacgg gctgtgcaag accgtcccgg
     2161 aatggcagtt gacgcaagtc ctcggcgccg aggatggcga ttcgccgacc gacggcctga
     2221 tcgctgccgc gctggcgttg aacgctaacg acaatgtcac ggcagtaacg gtcgaagccg
     2281 actgatcacc atgccgggct cccctgcctc ctcgaggccg aggggagcat ttcgtccatt
     2341 ccacgccacg agtgcccgcc tgccggcgtg ccctatggcg cggcgcgcag atcgtcgccc
     2401 tggtacaccc ccacccattg cagcacgtcg ccggcatgcg tgatcggcgc atccggcagc
     2461 cgccgcagcg cccggaaatc ggcgcccagt tccgctggaa cccgctcccg atgctccggc
     2521 gccagtacca gcacgtcgtg cccgagataa gcagcggccg gatgggcaaa gccgaactgc
     2581 cgggagtcgc ggttaaggca gagcaccgtc acctccggcc ccagcgcata ggcgatcttg
     2641 ccggcatccc gccagttcgg cacccccacc acggtgccgg aatgcagcag accgcgcgcg
     2701 gccaagtcgg cacggaccga gctccagtcg atcccctcca ccgtcggatc atgccgggcg
     2761 aaggccatga ccaccggctc cagccagccg aggctgagct gggtgccgat caccaccaat
     2821 gcgccaaccg ccagcgccgc agtgccgatc aggatccgcc gcaccaccgg atggccgatg
     2881 cgcagcgcca cggcttcacc aagcagcggg aacagcatca gatagccggg ggcggcccaa
     2941 tggaacagca cgcgctggct ggaccagacg gcgatggccg cgaacagcac gatcggcggc
     3001 atgcccagcc agcacagcag ccaacgccgc cagtcggcgg agccacgccg cagggcagcg
     3061 atccatgcca ccatcattgg cgcccagatc cagggcagca cgaacagggc ctcgccggcc
     3121 agcacgccga aggaagcgaa gggatggaac cgcagtccct ccgcgcggtc cccctggaag
     3181 gcgaaggagg cccaatgatg ggtggcgttc cacaccagca ccggagagaa caccagcagc
     3241 gcgacgaccc cggcaaggta gggctgcggc cgcgccagcc agtgccggtg cggggcgctg
     3301 gccagcaggt agagcccggc gcccagtacg gtcagcccgg cggagtattt tgagaacatc
     3361 gccaacccgg cgcacaggcc ggcggtggcc caccaccccc aggcggctcg gccacctgcc
     3421 ggcagggcgt gcagcatgca gagcgcggcg ccgagcagtg cgcagtccag cggcccgtcc
     3481 ggcagcaccc aggttgcggt ggtgatgccg aacaccggcg acaggttcac cagaaccgct
     3541 gcccacaaac ccgcgcgcgg cccggccaca gccgccccaa gccgcgccat cagccaggtg
     3601 gaaagcgcga acagggcgat gaagggcagg cgcaccacca ccggcgcctc cgagccgaag
     3661 agatgcgccg cgccccagga cagccaccac gccgcggggg gatggtcgaa atagcccaac
     3721 gacagcacgc ggccggcgga gatcatgtag ctctcgtcca cccccaggcc agtgctggcg
     3781 gcaaaagcga ggcggatggc ggtggtcgcc aggatcagcc agagcaggta ccggccggtc
     3841 gcaccatccc gtaccggggc cggcggtgct ggctgaacgc gtgccggcgg ggcgtcggtc
     3901 aggttttgca tgcgggacgc ctgcccgcac cggcggcctg acgtcaagcg ggaacggccc
     3961 ggcgccgtca gagcacaggt ccgttcagcg ctccgccgcc tggtacgccg ccaggcaggc
     4021 catatcgacg atctggctta ccgaggcgtc catcggcacc acctgcaccg gcttctccat
     4081 gccgatcagc agcggcccga tcgtcgtgcc gccgccgagc cggtgcacca gccgggaggt
     4141 gatcagcgcc gaatgcagcc ccggcatgat cagcacattg gcattgtcgg tcagccggca
     4201 gaacgggtac agcgcgcgga tcgacggctc caaggccacg tcggggctca tctcgccgtc
     4261 atattcgaaa tccaccttgc gctgatccag caccgccacg gcgtcgcgca tcggaatggc
     4321 gcgctcgttg ggtgggtcgc cgaacaccga atgcgacagc agcgccaccc gcggttcatg
     4381 tccgaggtgc cgtgccgcag ccgcagcgcc gacggcgata tcggccagtt gctccgcgtt
     4441 cgggcggaaa ttcatcgcgg tatcggcgac gaagatcgtc cggccccgca tgccgaccag
     4501 cagtgtcagc ccgaaagcca tgccgcccgg catcgggccg atcgccttgc cgatatcgtc
     4561 caggcacacg gcggcggaac gggtcagtcc ggtcaccatc gcatcggcat cgcccgtcgc
     4621 gaccatgcag gcggcgaaga cgttgcggtc ctggttgacc atgcgctggc agtcccgcac
     4681 caggtagcct ttgcgctgca ggcgccggta caggaactcc acatacttgc tgttcgcgct
     4741 ggacagccgg gcgttgtgaa tctcgatgcc ttccaccatg cccagcccga gactgttcat
     4801 ggtggactgc acccggtctt cgcgcccgat cagcacgggc gtgccatagc cggcattgcg
     4861 gaatgcgacg gcggcgcgca cgaccttttc ttcctcgcct tcggcgaacg ccacgcggcg
     4921 cgggttgtcg ctgacgctct ccatgatggc gtcaagcgcc gcggcggtcg gatccagccg
     4981 gctcgacagt tcgcgcgcat agcggcgcag gtcgctgatc gggcggcgcg ccacgccgct
     5041 gtccatggcc gcgcgcgcca ccgccggcgg cacggtggag atcagccgcg gatcgaaggc
     5101 gttggggatg atgtattccg gcccgaaact cagccgccgc ccggtggagg cgctgtgcac
     5161 ttcatccggc acatcctccc gcgcgagttt ggccaatgct tccgctgcgg caatcttcat
     5221 cgcgtcattg atcgtgctgg cgcgcacgtc cagtgcaccg cgaaagatat agggaaagcc
     5281 aagcacgttg ttcacctggt tcggatagtc gctgcggccg gtggcgatga tggcgtgcgg
     5341 actggcctcg cgtgcttcct ccggggtgat ttccggatcg ggattggcca tagcgaaaat
     5401 gaccggccgg tccgccatct gccgcaccgc ctgctggtcc accgcgccct tctgcgacag
     5461 gccgaagaac acgtcggcac cgtccagtgc ttcggccagc gtgcgcgcct gggtgggtgc
     5521 cgcgtgggcc gacttccact gattcatgcc ctcggtgcgg ccctcgtaga tcactccctt
     5581 ggtgtcgcac aggatgacgt tgtcggcgcg catgcccatc gacttcagca gctcgacaca
     5641 ggcgatgccc gccgcgccgg caccgttcac caccagcttc gt
//
LOCUS       DASSZH010000383         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_435147, whole genome shotgun sequence.
ACCESSION   DASSZH010000383 DASSZH010000000
VERSION     DASSZH010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435147"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="VFN42_08385"
     CDS             complement(<1..916)
                     /locus_tag="VFN42_08385"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET8996671.1"
                     /translation="MQYTTLGRTGLRVSVAGLGCGGFSKLGLGTGGSEDHAVALIRQA
                     LDLGVNLLDTAAVYGTEEVVGKALRGVPRDSVVVCTKASRAAGQAQFDPAAVLASLDD
                     SLRRLGLDFVDVFQVHAVPPSQYQQVRETIVPALQRAREAGKFRFLGITETAPNDVEQ
                     EMLNRAVQDKVWDTAMMAFHMMHQPARTRVFPGTQRNRIGTLLMFVVRGIFARPAQLA
                     AEVKQLVADGKLPAWLGKADNPLDFLLHEGGATSLTDAAYRYARHEPGADVVLFGTGD
                     ADHLRSNIASILRPPLPQSDRDMLRALFG"
     gene            complement(1057..>2073)
                     /locus_tag="VFN42_08390"
     CDS             complement(1057..>2073)
                     /locus_tag="VFN42_08390"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HET8996672.1"
                     /translation="AQGLPAFNVAASHSLYAAVLGPLAPQLNGVRSLIVAPTGPLLSL
                     PFAVLLTGPADSAHLSDAPWLIRRMTVVHVPSAENFVALRKIATTSRARQPWFGFGDF
                     RPVTLAQAKRTFPTSTCADSARLFAGLPSLPFAARELEAARVLLHGSREDELLGAAFT
                     VPNVMRAHLQDFRVLHFAAHALLSTDLRCQPQPAIVTSAPPGAVDASGALLTSDDVLG
                     LDLDADLVILSACNSGGPGGADAGDSLSGLARAFFFAGARALLVTHWSISDQSSAYLI
                     ADTLRRFADRSDGGLAGALRAAELGMIDGAGHALPAALAHPFYWAPFALIGEGHGQQP
                     SGRS"
BASE COUNT          382 a          735 c          655 g          301 t
ORIGIN      
        1 gaccgaacaa ggcccgcagc atgtcgcgat ccgactgggg cagcggcggg cggagaatag
       61 atgcgatgtt gctgcgcagg tggtcggcgt cgccggtgcc gaacagcacc acgtccgcgc
      121 cgggctcgtg ccgcgcatag cgatacgcgg catcggtcag gctggtcgcg ccgccttcat
      181 gcagcaggaa atcgagcgga ttgtcggcct tgcccaacca ggccggcaat ttcccatcgg
      241 ccaccagctg cttcacctcc gccgccaatt gtgccggacg ggcgaagatg ccgcgcacca
      301 caaacatcag cagcgtgccg atccggttgc gctgcgtgcc gggaaacacc cgcgtgcgcg
      361 cgggctggtg catcatgtgg aacgccatca tcgcggtgtc ccagaccttg tcctgcaccg
      421 cgcggttcag catctcctgc tccacgtcgt tcggcgctgt ctcggtgatg ccaaggaagc
      481 ggaacttgcc cgcttcgcgc gcccgttgca gcgccggcac gatcgtctcc cgcacttgct
      541 ggtattgcga tggcggcacg gcatgcacct gaaacacgtc gacaaaatcc aggcccagcc
      601 ggcgcaagga gtcatccagg ctggccagca cggcggcggg atcgaactgc gcctgcccgg
      661 cggcgcggga cgccttggtg cagaccacca cggaatcccg cggcaccccc cgcagcgcct
      721 tgcccaccac ctcctccgtg ccgtacacgg ccgcggtgtc cagcaggttc accccgagat
      781 cgagcgcctg ccggatcagc gccactgcgt ggtcttccga cccaccggtg ccaaggccca
      841 gcttgctgaa cccgccacag cccaggccgg cgacgctgac gcgcagaccg gtgcggccga
      901 gcgtggtata ctgcatggat cgtgtctccg gacctgcctg acggccatct cgccaacgca
      961 tcggccgtca acgctgatgg gccaccggtt tgagggtatg cttgttccgg tcggagcgcc
     1021 aatccggggg gatgagcccg tcgcggagtg gaaagtctag ctgcgtccgc tcggttgctg
     1081 tccgtgccct tcgccgatca gcgcgaacgg cgcccaatag aacggatgtg ccagggccgc
     1141 gggcagcgcg tgtcccgcgc cgtcgatcat ccccaactca gccgcccgca atgcccccgc
     1201 caaaccgccg tcgcttctat cggcaaaccg ccgcagcgta tccgcgatca ggtaagccga
     1261 ggactgatcg ctgatcgacc agtgcgtcac cagcaaagcg cgtgcgccgg caaagaagaa
     1321 ggcacgcgcc agccccgaca ggctgtcgcc cgcatccgcg ccccccggcc cgcccgaatt
     1381 gcatgccgac aggatgacca ggtccgcatc cagatccaac ccaagcacat catcgctcgt
     1441 gagcaaggcg cccgatgcat ccaccgcgcc cggtggcgcc gatgtaacga tcgccggctg
     1501 cggctggcag cgaaggtcgg ttgacagcag cgcatgcgcc gcgaaatgca gcacccgaaa
     1561 atcctgcagg tgcgcgcgca tcacattcgg tacggtaaaa gctgcaccca gcaattcgtc
     1621 ctcgcgtgat ccatgcagca ggacccgagc cgcttccagt tcgcgcgccg cgaacggcag
     1681 agacgggagc ccggcaaaca gccgtgcact gtcggcgcaa gtggaagtcg ggaacgtgcg
     1741 cttggcctgt gccagcgtca cgggacgaaa atcgccgaac ccgaaccacg gctggcgcgc
     1801 cctcgatgtc gtggcgatct ttcgcaacgc gacgaagttc tccgccgacg gcacgtgcac
     1861 cacggtcatc cggcgaatca gccatggcgc gtctgacaaa tgcgcactat cagccggccc
     1921 ggtgagcaaa accgcgaagg gcagcgatag cagcggcccg gtcggcgcca cgatcagcga
     1981 tctgacccca ttcaattgcg gcgcaagcgg ccccagcact gcagcgtaga gactatggga
     2041 cgcagcgaca ttgaaggcgg gcaggccctg cgc
//
LOCUS       DASSZH010000384         5124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_861307, whole genome shotgun sequence.
ACCESSION   DASSZH010000384 DASSZH010000000
VERSION     DASSZH010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861307"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VFN42_08395"
     CDS             <1..796
                     /locus_tag="VFN42_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HET8996673.1"
                     /translation="DEALRVALAALPDSAGPATIAATLAVGVPALLRAASGDAPIAPD
                     PTQATATDVLRMLHGRVPEQQAGQALDTYFTVMADSGMSTSSFTARAIASTRASLSAA
                     VTGAWCAFSGVLHGAAPGPTLDLLNAAAAAEDLDAWLEAKLRRGERLMGFGHRVYHGN
                     DPRAEAMRAALRRMPSAAGRLEFAALLEQRVAAVVERVKPGRRLPANVEVMAAILLDA
                     VGIPRQGFTAVFGVARCPGWIAHALEQQKTGRLIRPISRYIGPPVE"
     gene            868..1305
                     /locus_tag="VFN42_08400"
     CDS             868..1305
                     /locus_tag="VFN42_08400"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET8996674.1"
                     /translation="MSRQPPGRRADYRWFHAITTRWMDNDVFQHVNNVNYFSFFDTAV
                     TYYEMSEQVVGLFTGPTHCVVAEVACRYFRSLSFPDRVTVGVRVANIGRTSVRYELAV
                     FRNDEDDAAAEGHFVHVFVERGSQKPVPIPDESRTILQRIAVP"
     gene            complement(1412..2020)
                     /locus_tag="VFN42_08405"
     CDS             complement(1412..2020)
                     /locus_tag="VFN42_08405"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HET8996675.1"
                     /translation="MHAKTAVSALAIAFLGFAQAAYPAGSSNSQTALAKTVLHHSLQS
                     EAQQNAAEASLRCAAQARVSPQQAISVVARQTRGSVVEASFVMNGGMPSYAVRAVTGS
                     TLWRGYVSADSGAIRDGTAVAAADKAVDPGEKMQIRLVKRTKLPIGRAIGLAEHAVGG
                     RVLDALLVDARGRPEYQMQILTSGAIQSVVVDPAGGTVHLVG"
     gene            2202..2714
                     /locus_tag="VFN42_08410"
     CDS             2202..2714
                     /locus_tag="VFN42_08410"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HET8996676.1"
                     /translation="MSMRSAFLAAALVSGGFAATAGAQQLPGPRLIYTQPLAPSAVQS
                     VQSRLRSAGDNRGRIDGMWGPDSVTALERFQQTHGLQTTGQLNPATVATLGVWGPETQ
                     NALARFQQGRGLQANGQLNPATVAALGMSSGLVAERPTYQARHYAGTAEAGSPNCGTP
                     YHWKACAEKR"
     gene            2879..3661
                     /locus_tag="VFN42_08415"
     CDS             2879..3661
                     /locus_tag="VFN42_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8996677.1"
                     /translation="MDLGLKGRKAIVCAASKGLGKACAMSLAREGVDLVITARTAADL
                     ERTAAEIRQATGVTVTPVPGDITTEAGRAAALAACPAPDILVNNAGGPPRGDFREWGR
                     DEWIKAVDGNMLTPIFLIKGVIDGMIERKFGRIVNITSGSVKSPIPELGMSNGARAGL
                     TGFVAGLARQVAKHNVVINNLLPGPFLTDRLRSGFQEAAQKSGRPIDEVIADRSKTNP
                     TGRVGDPAEFGDACAFLCSAKIGFMVGQNLLLDGGAYNSSMG"
     gene            3689..>5124
                     /locus_tag="VFN42_08420"
     CDS             3689..>5124
                     /locus_tag="VFN42_08420"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET8996678.1"
                     /translation="MDPTFLPATQLAAKLRAGEIGCRELLDHYIARIERLDGRINAVV
                     VRDFERARARARELDSQSDRSAPLHGVPMTVKESFDVAGLPSTRGHLAAKDRAATASS
                     LSVQRLEAAGAVVFGKTNVPVDLADWQSYNPVYGATSNPWNLAHTPGGSSGGSAAALA
                     AGFSALEIGTDIGGSIRVPAHFCGVFGHKPTWTLCPSYGDPLTSAAAPIDIAVGGPMA
                     RSAADLDVALAAMAGPDPAETALRLDLPAPRFTSLKELRVAVWTQEPGQETDAELSAK
                     LEELADVLEREGATVSRTARPAFNATDAFHLYLKLLDAAWSARSTEELLAQKRAAAAS
                     LAPDDMSADAIMARCVDMPHRTWLGLNEQRGRLRRAWTAFFQEWDVLLCPPMATPALL
                     HMQQGATWERRYTVNGRSIPYNDMLFWPGLTCGYHLPASVVPIGRTTAGLPVGVQIAG
                     PLYGDRTTIAAARLLEEAGCAFVAPPGW"
BASE COUNT          806 a         1768 c         1717 g          833 t
ORIGIN      
        1 ggatgaggct ctgcgcgtcg cactcgcagc cctgcccgac agcgccggcc cggccaccat
       61 cgccgcgacc ctggcggtcg gcgttccggc gctgctgcgt gcggcatcgg gagacgcgcc
      121 gattgcgccc gaccccacgc aggccaccgc caccgacgtg ctgcgcatgc tgcacggccg
      181 cgtgccggaa cagcaggctg gacaggcact ggatacctat ttcaccgtca tggccgacag
      241 cggcatgagc acctccagct tcaccgcccg cgccatcgcc tccacccgcg ccagcctgag
      301 tgccgcggtg accggtgcgt ggtgcgcgtt cagcggcgtg ctgcatggcg ccgcgcccgg
      361 gccgacgctg gacctgctga acgccgccgc cgccgccgag gacctggatg cgtggctgga
      421 agccaagctg cgccggggtg agcggctgat gggattcggc caccgggtgt accacggcaa
      481 tgatccgcgc gccgaagcca tgcgcgcggc gctgcgacgc atgccctcgg ccgccggccg
      541 gctggagttc gcggcgctgc tggagcagcg cgtggcggcc gtggtggaac gcgtcaagcc
      601 gggccgccgc ctgcccgcca atgtggaggt catggcggcg atcctgctgg atgcggtcgg
      661 catcccgcgc cagggtttca ccgcggtctt cggcgtcgcc cgctgtcccg gctggatcgc
      721 ccacgccctg gagcagcaga agaccggacg gttgatccgg ccgatatcgc gctatatcgg
      781 cccgccggtg gaatagggca tccggatgga cgacgcgccc ctttcccggc tacctgaccg
      841 gcccacccac aagcacaccg aggacgcatg agccgccaac cacccggccg gcgggccgac
      901 taccgctggt tccacgccat caccacgcgc tggatggaca acgacgtatt ccagcacgtc
      961 aacaacgtga actacttctc gttcttcgat accgcggtga cctactacga gatgagcgag
     1021 caggtggtcg gcctgttcac cggtccgacg cattgcgtcg tggccgaggt ggcgtgccgt
     1081 tatttccgct ccctgtcgtt ccccgaccgg gttaccgtcg gcgttcgcgt ggcgaatatc
     1141 ggccgcacct cggtgcgcta cgaacttgcg gtcttccgca acgatgagga cgatgccgcg
     1201 gcggaaggcc atttcgtgca cgtgttcgtc gagcgcggca gccagaagcc ggtgcccatc
     1261 ccggacgaat cgcgcacgat cctgcagcgg atcgcagttc cctaaactct cgcgggcaac
     1321 gctggcacgt gctaccagcc cctggaaagc ccgactctcg ggctgccaga gtccgtcgtg
     1381 gggctctccc gcccattctg tcgggctggg atcagccgac caggtggacc gtccccccgg
     1441 cgggatcaac gacaacgctc tgaatcgcgc cgctggtgag tatttgcatc tgatattccg
     1501 gccgccctcg cgcgtccacg agcaaggcgt cgagcacacg cccgccaacc gcgtgttccg
     1561 ccaggccgat cgcgcgcccg atcggcagct tggtgcgttt caccaagcgg atctgcatct
     1621 tttcccctgg atccaccgct ttgtccgccg cggcgacggc cgtgccgtcg cgtatcgcgc
     1681 cgctatccgc gctgacatac ccgcgccata gcgttgagcc agtaaccgcc cggaccgcgt
     1741 agctcggcat gccgccattc atcacgaagc ttgcctccac cacgcttccg cgggtctgcc
     1801 gcgcaacgac ggagattgcc tgctgcggcg atactctggc ctgcgcagcg catcgcaggc
     1861 tcgcctcggc cgcgttctgc tgcgcctcgg actgcaggct atgatgcagc accgtttttg
     1921 cgagcgccgt ttggctgttg gagcttccgg ccggatacgc cgcctgggca aagcccagga
     1981 acgcgatggc caaggcggac acggcagttt tcgcgtgcat tgggtcccct cttcactgct
     2041 tcatgtgctc tcaatgaaat aagctccgct ctgttccctg ccatcagccg cttgacggca
     2101 acattcgctc gcgccttcgc gaatctcgtt gatttgtgaa cttcgccagt tcgcctccgt
     2161 tctggggcca gcacgagctt tcgctttggg aggagccggc aatgagcatg cgttcagcct
     2221 tcctcgctgc cgctctggtt tccggcggct tcgcggcaac cgctggcgcc cagcagcttc
     2281 ccggtccacg gctgatctac acgcagccgc tcgcaccatc cgccgtgcag tcagtgcagt
     2341 cacgcctccg ttccgccgga gataaccgcg gacggatcga tggcatgtgg gggccggaca
     2401 gcgtgaccgc gctggagcgg tttcagcaga cacacggttt gcagacgacc gggcagctca
     2461 atccggccac cgttgccacg ctgggcgtct ggggaccgga gacgcagaat gcccttgcgc
     2521 gcttccagca aggccgcgga ttgcaggcga acggacagct caatccggcg acagttgccg
     2581 ccctcggcat gtcatccggc ctggttgcgg aacgcccgac ctaccaagct cggcattatg
     2641 cgggcacggc ggaagcgggc tcgccgaatt gcggcacgcc ttatcattgg aaggcctgcg
     2701 ccgagaagcg gtaacggcgc ctctccctgc ctcgcgcctg cttgaccggc attgcgagac
     2761 cccggataca agccggatac ccgcatggcg cgggattgaa ggtgacgggc aacccactgc
     2821 ctcgcccgca gcatcgatcc ctcgcgcgat ccagggcgat tgaaaaggag ggggaagcat
     2881 ggacctggga ctcaagggca gaaaagccat cgtctgcgca gccagcaagg gcctgggcaa
     2941 agcctgcgcc atgtcgctgg cgcgggaagg cgtggacctg gtgatcaccg cacgcaccgc
     3001 cgccgacctc gaacgcaccg ccgcggaaat ccgccaggcg accggcgtca cggtcacgcc
     3061 ggtgcccggc gacatcacca ccgaagcggg ccgtgccgcg gcactcgccg cctgccccgc
     3121 tcccgatatc ctggtgaaca atgccggtgg gccgccgcgc ggcgatttcc gtgagtgggg
     3181 ccgcgacgag tggatcaagg cggtcgatgg caacatgctg acgccgatct tcctgatcaa
     3241 aggcgtgatc gacggcatga tcgagcggaa gttcggccgc attgtcaaca tcacctcggg
     3301 ttcggtgaag tcgccgatcc ccgagctggg catgagcaac ggcgcccgcg ccgggctgac
     3361 cggcttcgtt gccggcctgg cgcggcaggt cgcgaagcac aatgttgtca tcaacaacct
     3421 gctgcctggc cccttcctca ccgaccgtct gcgcagcggc tttcaggagg ccgcgcagaa
     3481 atcgggccgg ccgatcgacg aggtcattgc cgaccgctcg aagaccaacc cgaccgggcg
     3541 cgtgggcgat ccagcggagt tcggcgatgc gtgcgccttc ctgtgctccg cgaagatcgg
     3601 cttcatggtc gggcagaacc tgctgctcga cggtggcgcc tacaattcgt cgatgggctg
     3661 agaccgctcg atcgcggagg tgaaaccgat ggacccgacg ttcctgcctg cgacgcaact
     3721 cgccgcgaaa ctccgtgccg gcgagatcgg ctgccgcgaa ttgctggatc actacatcgc
     3781 ccggatcgag cggctggacg gccgcatcaa tgccgtggtg gtgcgcgatt tcgaacgcgc
     3841 ccgggcacgc gcccgggagc tggattcgca gtcggaccgc agcgcgccgc tccatggtgt
     3901 gccgatgacg gtgaaggaga gcttcgacgt ggccggcctg ccgagcacgc gcggccacct
     3961 ggcggcgaag gaccgggcgg caaccgcgtc gtcgctttcc gtgcagcgtc tggaggcggc
     4021 cggagcggtg gtgttcggca agaccaacgt gccggtcgat ctggcggatt ggcaaagcta
     4081 caacccggtg tacggcgcca catccaatcc atggaatctc gcgcacacac cgggtggctc
     4141 ctcgggtggg tcggcggcag cgctggccgc ggggttttcc gcactggaga tcggcaccga
     4201 catcggcggc tcgatccgcg tgccggcgca tttctgcggt gtgttcggcc ataagccgac
     4261 ctggacgttg tgcccgtcct atggcgatcc gctgaccagc gccgcggcgc cgatcgatat
     4321 cgcggtaggc ggaccgatgg cgcgttccgc cgccgatctc gacgtagcgc tggcggcgat
     4381 ggccgggccg gacccggcgg aaaccgcatt gcgtctcgac ctgccggcgc cgcgcttcac
     4441 cagcctgaag gaactgcgcg tcgccgtgtg gacgcaggag cccggccagg agaccgatgc
     4501 cgaactcagc gccaagctgg aagagctggc ggatgtgctg gagcgcgagg gcgccacggt
     4561 cagccggacc gcgcgtccgg ccttcaacgc cacggacgcg ttccatctgt acctgaagct
     4621 gctggatgcg gcctggagcg cccgcagcac cgaggaattg ctggcccaga agcgcgcggc
     4681 ggcggcgagt ctggcgccgg acgacatgag cgccgatgcc atcatggcgc gctgcgtgga
     4741 catgccgcac cgcacctggc tggggctgaa cgaacagcgc ggacgcttgc gccgtgcctg
     4801 gacggcgttc ttccaggaat gggacgtgct gttgtgcccg cccatggcaa caccggcgct
     4861 gctgcatatg cagcagggtg cgacctggga gcggcgctac acggtgaacg gacgctcgat
     4921 tccctataac gacatgctgt tctggccggg gctgacctgc ggctaccatc tgccggccag
     4981 cgtggtgccg atcggccgga ccacggccgg cttgccggtg ggcgtgcaga tcgccggtcc
     5041 gctctacggc gaccggacga cgattgccgc ggcgcgattg ctggaggagg cgggttgcgc
     5101 gttcgtcgca ccgccgggct ggga
//
LOCUS       DASSZH010000385         2269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1526322, whole genome shotgun sequence.
ACCESSION   DASSZH010000385 DASSZH010000000
VERSION     DASSZH010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526322"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VFN42_08425"
     CDS             complement(<1..546)
                     /locus_tag="VFN42_08425"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET8996679.1"
                     /translation="MSQNAAFAGLKIIDLTGGVAGPSCAMMFAQHGADVIKVETPHNG
                     GDWSRILGRRYQDHSAFSIYGTLGKRSLALDLKAPESKEILWRLLRGADVFMEGFRPG
                     TIQRYGFGYPEVSAREPQIIYYSISGFGQTGPLAARPAMDPVLQAMTGIVNENRGEHD
                     NHPHRIAISLIDMFTGLLGFQA"
     gene            756..1529
                     /gene="surE"
                     /locus_tag="VFN42_08430"
     CDS             756..1529
                     /gene="surE"
                     /locus_tag="VFN42_08430"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HET8996680.1"
                     /translation="MLDRILLTNDDGIDAPGLAVLEEIAAELAREVWVVAPEHDQSGV
                     SHAISLHHALRVSKQGERRFGISGTPGDCAVMGICHLMQDAPPQLLLSGVNRGLNLGM
                     ETVFSGTVGGAMTGMMLGVPSIAMSQAYRDRANPDWSASRTLGAQVVRRLLDMGWGKD
                     ACLNINFPPLPAAECGEMTLARQGVGMIAGMIVDTRVDPRGMAYHWLNFRRGDRVQGP
                     ESDYDAIRAGKVVVTPLRYDRTDEDAYRELAEKLPRISA"
     gene            complement(1650..1961)
                     /locus_tag="VFN42_08435"
     CDS             complement(1650..1961)
                     /locus_tag="VFN42_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996681.1"
                     /translation="MLADICRDLGVSPFRCETRFDDALHQAITTYGGDHTDLCCDMLN
                     RKIDLLAELERDRTPDLDWPKGDDPDTVRRVVGFLVGEAPVCPFGVPASPGIVEAAAP
                     S"
BASE COUNT          377 a          693 c          820 g          379 t
ORIGIN      
        1 cgcctggaac ccgagcaggc cggtgaacat gtcgatcaga gagatggcaa tgcggtgcgg
       61 gtggttgtca tgctcgccgc ggttctcgtt gacgattccc gtcatcgcct gcagcaccgg
      121 atccatggcc gggcgcgccg ccagcggtcc ggtctgcccg aagccggaaa tcgaatagta
      181 gatgatctgc ggttcgcggg cgctgacctc cggatagccg aagccgtagc gctggatggt
      241 gccgggccga aatccctcca tgaagacgtc ggcgccgcgc agcaggcgcc ataggatttc
      301 cttcgactcc ggcgccttca ggtccagcgc caagctgcgc ttgcccagcg tgccatagat
      361 cgagaaggcg gaatgatcct ggtaacgccg cccaaggata cgcgaccagt cgccgccgtt
      421 atgtggcgtt tccaccttga tcacgtcggc gccgtgctgc gcgaacatca tggcgcagga
      481 cgggccggca acgccgccgg tcaggtcgat gattttcagg ccggcaaagg cggcgttctg
      541 gctcatggtg tttcctgccc tggtgttctg ggcaggatgt tacggcgggg tgcgcccggg
      601 tggctatccc tggccccggc caagcggacg ccctgccgcc tggcttcagg cggcgtggca
      661 ctccacagct ccagcacgtc cgcgacccgc aacggtctct ggacgccggc ggggcgagcg
      721 gccaaacagg acgggaacct gaaggagaag ccggcgtgct ggaccgtatc ctgctgacca
      781 atgacgatgg catcgacgcc ccgggcctcg cggtgctgga agaaatcgcc gccgaactgg
      841 cacgcgaggt gtgggtggtg gcgccggaac acgaccagag cggcgtgtcg cacgcgatat
      901 cgctgcatca tgcgctgcgg gtcagcaaac agggggagcg gcggttcggc atctccggca
      961 cgccgggcga ttgcgcagtg atgggcatct gtcacctgat gcaggatgcg ccgccgcagc
     1021 tgttgctgtc cggggtgaat cgcgggctga acctggggat ggagacggtg ttctcgggga
     1081 ccgtcggcgg cgccatgacc gggatgatgc tgggcgttcc gtcgatcgcg atgagccaag
     1141 cctatcgcga tcgcgccaac ccggactgga gcgcgtcccg cacattgggc gcgcaagtgg
     1201 tacggcggtt gctggatatg ggttggggca aggacgcgtg cctcaacatc aactttcccc
     1261 cgctaccggc tgccgaatgc ggcgagatga cactggcgcg ccagggcgtg ggcatgatcg
     1321 ccggcatgat cgtggatacg cgcgtcgatc cgcgcggcat ggcctatcac tggctgaatt
     1381 tccgccgcgg cgatcgggtg cagggaccgg aaagcgacta tgatgcgatc cgcgcaggca
     1441 aggtcgtggt gacgccgctc cgttatgacc gcacggatga ggatgcgtac cgcgaattgg
     1501 cggagaagct gccgcgaatc agcgcgtaga gcgtggattc gtgttctggc cggcggcgtt
     1561 atccagatag ccgggacgcg aatccgtacg gcggggccgg ccggtgtccg gtcccgtgcg
     1621 tcatcgatgc ggttggcccg cgcgcgcggt caagacggtg cggctgcctc cacgattccc
     1681 ggtgatgcgg gcacgccgaa aggacagacc ggcgcctcgc caacaaggaa tcccacgacg
     1741 cggcggaccg tgtcgggatc gtcgcctttc ggccagtcga gatcgggagt cctgtcgcgc
     1801 tccagttccg ccagcagatc gatcttgcgg ttcagcatgt cgcagcacag atcggtgtga
     1861 tcgccaccat aggtggtgat cgcctgatgc agcgcgtcgt caaaccgggt ttcgcagcgg
     1921 aacggcgaga cgccgaggtc gcggcagata tccgcgagca cctggccaac agattggccg
     1981 tgatttccgc ttcgaactgc gcgacggtgg gaatgctgtc gaggtcggtg gtttcctccg
     2041 gatcggggcg gacagcgggt tcggacgtgg atgattgacg tccgtccgct gccgcggatg
     2101 accgctcccg cgacggccgg taaggggcag ggtgctcggg gaccaggtcg ttgccggtcg
     2161 cggcccgcgc cagtagcacg cgctgcaacg ccatggcgcg gcggatgccg cgggtcagac
     2221 gagcgagaat gacgggaagc cgcgcggtgc cgaagcactg cgcgatggt
//
LOCUS       DASSZH010000386         2484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1509290, whole genome shotgun sequence.
ACCESSION   DASSZH010000386 DASSZH010000000
VERSION     DASSZH010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1509290"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VFN42_08440"
     CDS             complement(<1..313)
                     /locus_tag="VFN42_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET8996682.1"
                     /translation="MNDAPAQRDYRDTVFLPQTSFPMRGDLPKKEPQILARWDAMDLW
                     SRLREASKGRKLFILHDGPPYANGNIHIGTALNKILKDVINRTRQMAGYDANYIPGWD
                     CH"
     gene            831..1421
                     /locus_tag="VFN42_08445"
     CDS             831..1421
                     /locus_tag="VFN42_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996683.1"
                     /translation="MKISGSKRTVARALSLSAIGLTLSATAVFAQPQWHSKVLAQKQV
                     KALPSGPLYWEVQNFTSPAAAKSAAQPTSLEVQVGGSAWLFTLAPKGTTISGGSKFAE
                     IGPVEAVKAPAYRLRVAIAGGPPGAKMPAHKTPATDAFYVLTGQVTAGATKADAGKSL
                     AAGASGAVSSSGKQDLDAFVLSVSATKAQMAAAKTK"
     gene            complement(1495..1899)
                     /locus_tag="VFN42_08450"
     CDS             complement(1495..1899)
                     /locus_tag="VFN42_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET8996684.1"
                     /translation="MALTRSFRDLVQHRVANDPGFGREMLREAIACMLAGDLEAGKAL
                     LRDYIKATLGFEKLGMAVGAQPKSLIRMFGPRGNPQARNLFRVIGYLQKQAGMELSLV
                     DKAASARPPGAAGTRRAPHSREKPSCAPVAAR"
     gene            complement(1890..2252)
                     /locus_tag="VFN42_08455"
     CDS             complement(1890..2252)
                     /locus_tag="VFN42_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET8996685.1"
                     /translation="MLPSEPLELRYYATEQGHSPFERWFLGLDQAAAARVVTALERLS
                     RGNLSNTKAVGAGVLEHRIDWGPGYRVYFGRDGEKLVVLLMGGTKQRQPRDIDTAKLF
                     WADYKQRRDRRGGVRLWH"
BASE COUNT          421 a          831 c          827 g          405 t
ORIGIN      
        1 cgtgacaatc ccagccgggg atgtagttgg cgtcataccc ggccatctgc cgcgtgcggt
       61 tgatgacgtc tttcaggatc ttgttgagcg ccgtgccgat atggatgttg ccgttcgcat
      121 agggcgggcc gtcatgcagg atgaacagct tgcgaccctt ggacgcctcg cgcaggcggg
      181 accacagatc catggcgtcc cagcgggcga ggatctgcgg ctccttcttc ggcaggtcgc
      241 cacgcatggg gaagctggtc tgcggcagga agaccgtgtc gcggtaatcg cgctgggcgg
      301 gggcgtcgtt catggcaggt tccgagcggc atgcgccgcg ctggaggggc agacaggcac
      361 tatggtcccg gggctctagt gagccgccgg gccggtaatt cgcgcctgcc tatacacgga
      421 tgccatggca cggaatatgt gaagccgggg gcgggcaggt caagcgaccg agcgccgcca
      481 aggaattgag ggcaaacaag tgcttcaatc ggcgcgggca cgacacacgt gtcatggccg
      541 ggcttggccc ggctacccac ggctgccctt gttgtcagct tcgaagtcgt gggtggccgg
      601 ccttcgccgg ccatgacaca aatgacgcga gccgccccgg caccgcctcg attatgcgca
      661 acgacgccga agcggtgccc gacctacctg ttgctggcga cgctgttccc tacccgcagc
      721 ggctgagaag gtctatcacg ccggcatctt cagtggcata ccgccttcct ccggcgatgg
      781 tatcgcaacc aaggcgtacc tgtcggatgt cgcaaggaag agaattcagc atgaagatat
      841 ccggttccaa gcgcaccgtt gcccgggcgc tgtcactgtc cgcgatcggg ttgacgctgt
      901 cggcaacggc ggtgttcgcc caaccgcaat ggcacagcaa ggtgctggcc cagaagcagg
      961 tcaaggcgct gccgtccggc ccgctgtact gggaggtgca gaacttcacc tcgcccgcgg
     1021 cggccaaatc ggccgcgcag ccgacctcgc tggaggttca ggtcggtggc tccgcctggc
     1081 tgttcaccct cgcgccgaag ggcaccacga tttccggcgg cagcaaattc gccgaaatcg
     1141 ggccggtgga ggcggtgaag gcgccggcat accggttgcg cgtggcgatt gccggcggac
     1201 ctccgggggc gaagatgccg gcgcacaaga cgcccgctac cgacgcgttc tatgtgctca
     1261 ccggccaggt gaccgccggc gcaaccaagg ccgatgccgg caagtcgctg gcggccgggg
     1321 catcgggcgc ggtgtccagc agcggcaagc aggacctgga cgcgttcgtc ctgtcggtga
     1381 gcgcgaccaa ggcgcagatg gcggcggcga agacgaagta ggatggtcgt cacccggcgc
     1441 cgggggtcat ccggcgccgg gtggctggct gtcagccgat cgcaacccgc tgcattaccg
     1501 ggcggcgacc ggcgcacagg atggcttctc ccggctgtgc ggggcgcggc gtgtgccggc
     1561 cgctccgggt ggacgcgccg aggcggcctt gtccaccagc gacagttcca tgcccgcctg
     1621 cttttgcaga tagccgatca cacggaacaa gttgcgcgcc tgcggattcc cgcgcgggcc
     1681 aaacatgcgg atcaggctct ttggctgcgc cccaacggcc atcccgagtt tctcaaaccc
     1741 cagggtggcc ttgatgtagt cgcgcagcag cgccttgccg gcctccagat ccccggcgag
     1801 catgcaagcg atcgcctcgc gcagcatctc ccggccgaag ccgggatcat tggcgacccg
     1861 gtgctgcacc aaatcccgga agctccgcgt caatgccata gccgtacccc tccgcgccgg
     1921 tcccggcgct gcttgtagtc agcccagaag agcttggccg tgtcgatgtc ccgcggctgc
     1981 cgctgcttgg tgccgcccat cagcagcacc accagctttt cgccatcccg cccgaaatac
     2041 acgcggtaac ctggtcccca gtcgatccga tgctccagta cgccggcacc cacagccttg
     2101 gtgttggaga gattgccacg cgacaggcgc tcgagcgccg tcaccacccg tgcggccgcc
     2161 gcctgatcga gaccaaggaa ccaacgctcg aatgggctgt ggccctgctc ggtcgcgtag
     2221 tagcgcagct ccagcggctc tgacggtaac acaagtgcta ctctggtcaa ggcccgggga
     2281 accgggccga gggaacgacc agtccgttat cgaaggctca ggtaaaggga cgatgaacca
     2341 gcgcggcggg attctcgcgc ggtcacccgc cgaaccgcgc atccggataa tcgatcaccc
     2401 gcacttcctc actgcccgta tccagcaccg cgcaactgag ctgccgtcca ttgcggtggt
     2461 cgcgcgcatc ccccgccgat ccgg
//
LOCUS       DASSZH010000387         3820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1185198, whole genome shotgun sequence.
ACCESSION   DASSZH010000387 DASSZH010000000
VERSION     DASSZH010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185198"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="VFN42_08460"
     CDS             complement(<1..934)
                     /locus_tag="VFN42_08460"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007398980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HET8996686.1"
                     /translation="MPELFLELFSEEIPARMQARAGDDLARLAAEALAALRPCGLRSF
                     YGPRRIALVAQVVAEVAASSSIERGPRATAPEQALAGFLRKHNASRDQLRQDGDYWVL
                     EKTAAAVTAPALIAGALPALLRRFPWPKSMRWGGSSAFTWVRPLRRIVCLLDGAVVPF
                     TLRDGTDDGHGLAAGDQTEGHRFLAPGAFPVSSAADWAGKLRTHRVLPEAEERKRVIA
                     EGIHALAADRQLTVVDDPGLLDEVAGLVEWPVPLLGRIAQEHMDLPPEVMQVSMRVNQ
                     RYFALRSPDGRAAPYFAFVANVEAPDGGAAIVAGN"
     gene            complement(957..1205)
                     /locus_tag="VFN42_08465"
     CDS             complement(957..1205)
                     /locus_tag="VFN42_08465"
                     /inference="COORDINATES: protein motif:HMM:NF019324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HET8996687.1"
                     /translation="MRDELRIKGEEACILAAELMALTGESLDWAVTAALRERFEREQA
                     RRSWHDRIMLITREIAQTGGEMPANDRTADTGSRALAG"
     gene            1491..2429
                     /locus_tag="VFN42_08470"
     CDS             1491..2429
                     /locus_tag="VFN42_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET8996688.1"
                     /translation="MSSGNITLPAVLRPAAATRQRRRARPALLAAWLLAPSGLVLLAF
                     TYWPVLQAILASVTVRSFGTAPHWGLGNYARLFADPHFGRAVVNNVVYAAGTIIPSLV
                     LALGFALMLRESTRFAVVLRTLIALPMLIPLVAAASLFIFIFLPGGGLLDYYLTKLGL
                     GWGQTNWLGDPSLALGSIIAITVWKNTGYYMLFFLAGLAGIPQDFLDAARIDGARPFA
                     RFRHVTLPLLGPTLAFVLVIALLNVLTQVDHVVVMTQGGPDDSTALLLYYIYQQAHQN
                     YDIGLASAATVVSVAVLFLLSIVSLRSLERGIHYES"
     gene            2431..3246
                     /locus_tag="VFN42_08475"
     CDS             2431..3246
                     /locus_tag="VFN42_08475"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET8996689.1"
                     /translation="MSRKLVWLLLAAAAVLWAVPFAWMAVASLRPGSAGLADIGALFP
                     AGPFDTANFAEAWQSGNFPLWYLNTIIMCGGILLVQCVTVSLAGYAFARLNFRGKTTL
                     FYLFLLQLMLVPPILIVPNMTTLISLHLYDTLLGVMVPYFVSAFGVFLMRQTFRSIPR
                     DYEEAAMLDGAPVLTLIFRVLLPLARPGLIAFAIVSLTAHWNEFLWPLMATSSPSSQV
                     LTVGLASFTSGAEAGSEWGVMAAGTFLVAAPLLVLFVAFQRQIVASFTFSGIK"
     gene            3261..>3820
                     /locus_tag="VFN42_08480"
     CDS             3261..>3820
                     /locus_tag="VFN42_08480"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8996690.1"
                     /translation="MRLLSAATIALALSFGSAQAATELDMFFPVPVQGKLATQMQHLI
                     EQFDQTHPDIHVTAVYTGSYDDTNLKTRAAIQANHPPGLVLMSANFIREYAINNDAIP
                     LDSLIAQSGQTPNQYMAEFWPALKLNAMEQGHVYGVPFQNSTPLLYYSVDAFKQAGLD
                     PNKPPVTWQDWVDDMRKLTVRDGDTT"
BASE COUNT          541 a         1313 c         1290 g          676 t
ORIGIN      
        1 cgttgccggc gacgatcgcc gcgccgccat ccggcgcctc cacgttggcg acgaaggcga
       61 aatacggcgc cgcgcggccg tccgggctgc gcagcgcgaa gtagcgctgg ttcacccgca
      121 tggagacctg catcacctcc ggcggcaggt ccatgtgctc ctgggcgatg cggccgagca
      181 gggggacggg ccattcgacc agtccggcca cctcgtcgag caggccggga tcgtccacca
      241 cggtgagctg gcggtccgcg gccagggcgt ggatgccctc agcgatcacc cgcttgcgct
      301 cctctgcttc cggcagcacg cgatgggtgc gcagcttgcc ggcccagtcg gcggcggagc
      361 tgaccgggaa ggcgccgggg gcgaggaagc ggtggccctc ggtctggtcg ccggcggcca
      421 ggccgtggcc gtcatcggtg ccgtcgcgca gggtgaaggg caccaccgcg ccgtccagca
      481 ggcagacgat gcggcgcagc ggccgcaccc aggtgaaggc gctggagccg ccccagcgca
      541 tcgatttcgg ccagggaaac cggcgcagca gggcagggag ggcgccggcg atcagcgccg
      601 gggcagtaac ggccgcggcg gtcttctcca gcacccagta atcgccatcc tgccgtagct
      661 gatcgcggga ggcattgtgc ttgcgcagga acccggccag cgcctgctcc ggcgcggtgg
      721 cgcgcggacc gcgctcgatg ctgctggaag ccgccacctc ggccaccacc tgcgccacca
      781 gggcgatgcg gcgcggcccg tagaagctgc gtaacccgca cgggcgcaac gccgccagcg
      841 cctcggcggc cagccgggcg aggtcgtcgc ccgcgcgggc ctgcatgcgc gccggaattt
      901 cctcgctgaa gagttcgaga aacagttccg gcatgcgctg tccttgctgt tgctgcttat
      961 cccgccagcg cgcggctgcc ggtatcggcg gtgcggtcgt tggcgggcat ttcgccgccc
     1021 gtctgggcga tttcgcgggt gatcaacatg atgcgatcat gccaggagcg gcgcgcctgc
     1081 tcccgctcga accgctcgcg cagcgccgcg gtcaccgccc aatccagcga ctctccggtc
     1141 agcgccatca gttcggccgc caggatgcat gcttcctcgc ccttgatccg caattcgtcg
     1201 cgcatggcgc ttcctctcgg gtttctggtt gcggcggcgt tttaggcggc aaccgggcgg
     1261 ttgaccagcc catcgccgcg gtttccgcgg ccggcggagg gggcaggcaa tgcccgacaa
     1321 tgatcggctt gcccgccgtg tggcaccaaa tgccgtcatg gccagcccac gggtcgtgcc
     1381 cgagaaaggg cgccacactg tagcgctgat ccctgcggat cgcctaagct gtcatgcaat
     1441 cgcagcctga tcgtcacgcg cctgtcagca gcggaggcct aagtggccgg gtgagcagcg
     1501 gcaacatcac actcccggca gtcctccgcc cggcggcggc cacgcggcag cggcgtcgcg
     1561 cgcgtcccgc gctgctggcg gcctggctgc tggcgccgtc cgggctggtg ctgctggcct
     1621 tcacctactg gccggtgctg caggcgatcc tggcctcagt gaccgtgcgc agcttcggca
     1681 ccgcgccgca ttggggactg ggcaattacg cccgcctgtt tgccgatccg catttcggcc
     1741 gcgccgtggt caacaacgtc gtctatgcgg ccggcacgat catccccagc ctggtgctgg
     1801 ccctgggctt cgcgctgatg ctgcgggaaa gcacgcgctt cgcggtcgtg ctgcgcacgc
     1861 tgatcgcgct gccgatgctg atcccgctgg tcgccgcggc ctcgctgttc atcttcatct
     1921 tcctgccggg cggcggcctg cttgactatt acctgaccaa gctcgggctc ggctgggggc
     1981 agaccaactg gctgggcgac ccctcgctgg cgctgggctc catcatcgcc atcacggtgt
     2041 ggaagaacac cggctactac atgctgttct tcctggccgg gctggccggc attccgcagg
     2101 atttcctcga cgcggcgagg atcgacggcg cccggccctt cgcccgcttc cgccacgtca
     2161 ccctgccgct gctcggcccg acgctcgcct tcgtgctggt gatcgcgctg ctgaacgtgc
     2221 tgacgcaggt ggaccacgtg gtggtcatga cgcagggtgg accggacgat tccaccgcgc
     2281 tgctgctgta ctacatctac cagcaggcgc atcagaacta cgacatcggc ctggcgtccg
     2341 ccgcgacggt ggtcagcgtg gcggtgctgt tcctgctgtc catcgtgtcg ctgcgcagcc
     2401 tggaacgcgg catccactat gagagctgat gtgtcgcgca agctggtgtg gctgctgttg
     2461 gccgccgccg cggtgctgtg ggcggtgcct ttcgcctgga tggcggtggc cagccttcgc
     2521 ccaggcagcg ccggcctggc cgatatcggc gcgctgttcc ccgccggccc gttcgacacc
     2581 gccaacttcg ccgaggcgtg gcagtcgggg aatttcccgc tgtggtacct gaacaccatc
     2641 atcatgtgcg gcggcatcct gctggtgcag tgcgtcaccg tcagcctggc cggctatgcc
     2701 ttcgcgcggc tgaacttccg cggcaagacc acgctgttct acctgttcct gctgcagctg
     2761 atgctggtgc cgccgatcct gatcgtgccc aacatgacca cgctgatctc gctgcacctg
     2821 tacgacacgc tgctgggcgt catggttccg tatttcgtct cggccttcgg cgtgttcctg
     2881 atgcgccaga ctttccgcag catcccgcgc gactatgagg aagcggcgat gctggacggc
     2941 gcgccggtgc tgacgctgat cttccgcgtg ctgctgccct tggcgcgccc gggcctgatc
     3001 gccttcgcca tcgtgtcgct gaccgcgcac tggaacgagt tcctgtggcc gctgatggcg
     3061 acatcctcgc catcgtcgca ggtgctgacg gtgggcctgg catccttcac ctccggcgcg
     3121 gaagccgggt cggagtgggg cgtgatggcg gccgggacat tcctggtcgc cgcaccgctg
     3181 ctggtgctgt tcgtcgcttt ccagcggcag atcgtcgcca gttttacgtt ttccggaatc
     3241 aaatagggga gtcttcacgc atgcgattgc tgtctgccgc gacgattgcg ctcgcgttgt
     3301 ccttcgggtc ggcgcaggcc gccaccgagc tggacatgtt ctttccggtg ccggtgcagg
     3361 gcaagctggc cacccagatg cagcacctga tcgagcagtt cgaccagacg catcccgaca
     3421 tccatgtcac cgccgtctat accggcagct atgacgacac caacctgaag acgcgggcgg
     3481 cgatccaggc caaccatccg ccgggcctgg tgctgatgag cgccaatttc atccgcgaat
     3541 acgcgatcaa caatgatgcg atcccgctgg atagcctgat cgcgcaaagc gggcagacgc
     3601 cgaatcagta catggccgag ttctggccgg cgctgaagct gaacgcgatg gagcaggggc
     3661 acgtgtacgg cgtgccgttc cagaactcca cgccgctgct gtactacagc gtggacgcgt
     3721 tcaagcaggc cgggctggat ccgaacaagc cgccggtgac gtggcaggac tgggtggacg
     3781 acatgcgcaa gttgacggtg cgcgacggcg ataccaccaa
//
LOCUS       DASSZH010000388         6905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_980736, whole genome shotgun sequence.
ACCESSION   DASSZH010000388 DASSZH010000000
VERSION     DASSZH010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6905
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980736"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2237)
                     /locus_tag="VFN42_08485"
     CDS             complement(<1..2237)
                     /locus_tag="VFN42_08485"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019085228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HET8996691.1"
                     /translation="MAGVDILASAFSGANAAFLAEQYARWATNPASVDPSFADLFSAL
                     NDEARSVLEEATGASWAPRGGVAEAEAAAPRPTAAATPTIALSNAQVRAATIASLRAL
                     MLIRSYRVRGHLEARLDPLGLVVPPPHPELDYRSYGFTDADLDKPIFIDNVLGRETAT
                     LREIVQILRDTYCGAIGVEFMHIQDPEQKSWIQRKVEGAPWHRAFDARDKQQILQQLT
                     EAEGFEVFCQKRYVTTKRFGLEGGEITIPALHAIIQTAAQAGVQEIAIGMPHRGRLNT
                     LVNVVKKPFTAVFSEFEGGSFKPDDVQGSGDVKYHLGTSTDIEVGGHTVHLSLQPNPS
                     HLEAVDPVVVGKVRARQDQGGDTQRRSSVMAILMHGDAAFAGQGTVYETLAMSQLIGY
                     RTGGTVHVVVNNQIGFTTVPAHAYSGLYCTDVAKSIQAPILHVNGDDPEAVVFCARMA
                     TEFRMRFATDIVLDIVCYRRHGHNETDEPAFTQPVMYRAIGQTKTTRTLYAERLAAEG
                     VMAAEDSKAIWDAFTEAMNQAYQAAQSYKPNKAEWLEGHWTGFQAADAEFERHEEDTA
                     VSEHVLRQVGMALVRVPAGFDVNPKIARQLEAKQAALDSGEGIDWATGEALAFGTLLL
                     EGSRVRLSGEDTQRGTFSQRHAVLIDQTNQHEYVPLNNIAPDQAKIEIYNSLLSEVGV
                     LGFEYGYSLADPRTLVLWEAQFGDFANGAQTIIDEFVSSAEQKWGQRSSLVLLLPHGY
                     EGQGP"
     gene            complement(2480..4063)
                     /locus_tag="VFN42_08490"
     CDS             complement(2480..4063)
                     /locus_tag="VFN42_08490"
                     /inference="COORDINATES: protein motif:HMM:NF045090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET8996692.1"
                     /translation="MRAIDPPRDRTIAQPGLAPSAPAGEAMEALLQLGYGAVARGERA
                     RAAAILDRVARERPDQPHPCAGLARALPKVPRSRIVEQFRASLRLAPDDLRLCEAYAG
                     FLLDGDQAEQALPVLHSWLERQPNAAAAHTMAGIALNALGRFGAALDHLQCAVSLAPG
                     QAACWANLGMLLKTVGDFRPALNAYAEAVARDPDNPRLRVNRAVALLHAGCWPEAWTD
                     YEYRLLLPGRHALAGLGPLLPTLSPTVSLEGRSVLVTHEEGFGDTIQFARYVPLLAQH
                     GARVLVWVPAPLERLFAPLPGIAELLVGNLPVPPHDFHCPMLSLPRAFGATTATVPNA
                     PYLRPAADLVAAWAGRVPRDGLRVGLAWAGQSRPWLPDFAALDRRRSAGLAALAPLAA
                     VPGIRLVSLQTGPAAAEAAAPPARLQVDDHSTALTDFAETAALIANLDLVISVDTAVV
                     HLAGAMGKPVFLLDRYDNCWRWLSGRADSPWYPSMTIFRQSQPGDWTEPVSRAAAALE
                     AMALFRGAEQARVLPAGQA"
     gene            4214..4975
                     /locus_tag="VFN42_08495"
     CDS             4214..4975
                     /locus_tag="VFN42_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET8996693.1"
                     /translation="MSLFLRLAPGLFGLLALAAWEAAVRITHVPVYLVPGPIAIVGAF
                     LADPAGLLMSLLSTLTVTFAALLVAATLGSAMAVAMALSRLAQAAIQPWAVVLQVTPI
                     VAIAPLIIVWVGDPFTSLVVCATIVAFFPVFANTAAGLSSTAAELSDLFRLYGAGRWK
                     TLWLLRLPAALPYFLAGLRISGGLALVGAVVAEFVAGSGGFASGLAYRILEAGYRLEV
                     ARMFAALVLLSLAGIAINAALGAFGRAIMRRRGEA"
     gene            complement(4979..6277)
                     /locus_tag="VFN42_08500"
     CDS             complement(4979..6277)
                     /locus_tag="VFN42_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HET8996694.1"
                     /translation="MSEVARTTLSDRTTRAVRDALAGQRGGLRTAIAVSGPAVIASVA
                     YMDPGNFATNIQAGSAYGYGLLWVVVVANLVAMLFQAMSAKLGIVTSHNLAELCRIHF
                     RPPVVISMWVASEIAAMATDLAEFLGGAIGLSLLLHIPLLWGMGITGAVTYGLLMLEH
                     RGFRPIELAIGGLVLTIGICYIVELLIVPPDWAAAARHSVLPHLPDGAAATLAVGIMG
                     ATVMPHAIYLHSALTQYRVPARTNAERRQLLRFSNREVVVALTFAGIVNMAMVMMAAA
                     AFHAGHPDVAEIGSAYHILGPLLGAGAAAAFLVSLMASGISSSTVGTMAGQIIMQGFV
                     GFRIPLWLRRLITMAPPFFVVAMGYNATMSLVISQVVLSLVLPVPVIAMLVLSRRRAV
                     MGEFVTGPIAFAAACAATLVVLLLNLVLLLQTFGVPVPGL"
     gene            6524..>6905
                     /gene="mntR"
                     /locus_tag="VFN42_08505"
     CDS             6524..>6905
                     /gene="mntR"
                     /locus_tag="VFN42_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HET8996695.1"
                     /translation="MSGTSDLPRLQVPIELPEEPTQALRFGKTRNERSTALLEDYVEL
                     IADLLASNGEARTIDIARRLGVTHTTAVKSLARLKREGLATSRPYRGVFLTEAGRVLA
                     ERVRARHRLVVDLLVAVGVPPEAAE"
BASE COUNT         1040 a         2345 c         2423 g         1097 t
ORIGIN      
        1 tcgggaccct ggccttcgta gccgtgggga agcagcagca ccagtgagga gcgctggccc
       61 catttctgct cggccgacga cacgaactcg tcgatgatcg tctgcgcacc gttcgcgaag
      121 tcgccgaact gcgcttccca cagcaccagg gtgcgcggat cggccagtga gtagccgtac
      181 tcgaagccca gcacgcccac ttccgacagc agcgagttgt agatctcgat cttcgcctga
      241 tcgggcgcga tgttgttcag cggtacgtat tcgtgctgat tggtctggtc gatcagcacg
      301 gcatggcgct ggctgaacgt gccgcgctgg gtgtcctcgc cggacaggcg cacgcggctg
      361 ccttccagca gcagcgtgcc gaacgcgagc gcctcaccgg tcgcccagtc gatgccctcg
      421 ccgctgtcca gtgccgcctg cttggcttcc aactggcggg cgatcttcgg gttgacgtcg
      481 aaccccgccg gcacgcgcac cagcgccatg ccgacctgcc tcagcacgtg ctcggatacc
      541 gcggtgtctt cctcgtgccg ttcgaactcg gcatcggccg cctggaaacc ggtccaatgg
      601 ccttccagcc actcggcctt gttcggcttg tacgactggg ccgcctggta ggcctggttc
      661 atggcctcgg tgaaggcgtc ccagatcgcc ttggagtctt ccgccgccat cacgccttcc
      721 gccgccagcc gttcggcgta cagcgtgcgg gtggtcttgg tctggccgat cgcgcggtac
      781 atcaccggct gggtgaaggc cggctcgtcg gtctcgttgt gcccgtgccg gcggtagcag
      841 acgatgtcca gtacgatgtc ggtggcgaag cgcatgcgga attccgtcgc catgcgggcg
      901 cagaacacca ccgcttccgg atcgtcgccg ttgacgtgca ggatcggcgc ctggatcgac
      961 ttcgccacgt cggtgcaata caggcccgaa taggcgtgcg cgggaacggt ggtgaagccg
     1021 atctggttgt tcaccaccac gtgcaccgtg ccgccggtgc ggtagccgat cagctggctc
     1081 atcgccagcg tctcgtacac cgtgccctgg ccggcgaagg ccgcgtcgcc atgcatcagg
     1141 atcgccatca ccgagctgcg ccgctgcgtg tcgccgccct ggtcctggcg cgcgcggacc
     1201 ttgcccacca ccaccggatc gacggcttca aggtgagacg ggttgggctg caacgacagg
     1261 tgcacggtgt ggccgcccac ctcgatgtcg gtggaggtgc ccaaatggta tttcacgtcg
     1321 ccggagccct gcacgtcgtc cggcttgaag ctgccgcctt cgaactcgct gaacaccgcg
     1381 gtgaaaggct ttttcaccac gttcaccagc gtgttcaacc ggccgcgatg cggcatgccg
     1441 atggcgatct cctgcacgcc ggcctgtgcc gcggtctgga tgatggcatg caatgccggg
     1501 atggtgattt cgccgccttc caggccgaag cgcttggtgg tgacatagcg cttctggcag
     1561 aacacctcga agccttccgc ttcggtcaac tgctgcagaa tctgctgctt gtcgcgtgca
     1621 tcgaaggcgc ggtgccaggg cgcgccttcc accttgcgtt ggatccagga tttctgttcc
     1681 gggtcctgga tgtgcatgaa ctcgacgccg atcgcgccac aataggtgtc gcgcaggatc
     1741 tgcacgatct cccgcagcgt cgcggtttcc cggcccagca cgttgtcgat gaagatcggc
     1801 ttgtccaggt cggcgtcggt gaagccgtag gaccggtagt ccagctcggg atgcggcggc
     1861 ggcaccacca ggcccagcgg gtcgagccgc gcttccagat ggccgcgcac gcggtaggag
     1921 cggatcagca tcagcgcgcg caggctggcg atggtcgctg cgcgcacctg cgcgttggac
     1981 agcgcgatgg tgggcgttgc ggcggcggtg ggccgcggcg cggcggcctc cgcttcggcg
     2041 accccgccgc gcggtgccca gctggcgccg gttgcttcct ccagcaccga ccgcgcctcg
     2101 tcattcagcg cgctgaacag gtcggcgaag ctgggatcga ccgaggccgg gttggtggcc
     2161 cagcgggcat attgttctgc cagaaacgcg gcgttggctc cgctgaaggc ggaagccagg
     2221 atatcgacgc cagccatgcg cttggattcc tcgtgtgacg cggcgcggtg aataggcttg
     2281 cccacccggg cgggaaaccg cgcccaggca gcgcagggtt gctcttaccg catgccgtgc
     2341 aagtgtcaca cgcacgcgac cgggagctca tgctcgcggc gcggtgggat gacggtccgg
     2401 cggtgcgatc ggaggctaac gatccagatg cgccgctatt cccgccattt caagcgaccg
     2461 gaacgatcgt ttgcgcggtt cacgcctgtc ccgccggcag gaccctggcc tgctcggcgc
     2521 cgcggaacag cgccatcgcc tccagagccg cggcggcgcg ggagaccggc tccgtccagt
     2581 caccgggttg gctctgccgg aagatcgtca tgctggggta ccagggactg tcggcccgcc
     2641 cgctgagcca gcgccagcaa ttgtcatagc gatcgagcag gaacaccggc ttgcccatcg
     2701 ccccggccag atgcaccacc gcggtatcga cgctgatcac caggtccaga ttggcgatca
     2761 aagccgcggt ctcggcaaag tcggtcagcg cggtgctgtg gtcgtccact tgcagcctgg
     2821 cgggcggcgc agcggcttcc gccgcggcgg gaccggtctg caggctgacc aatcggatgc
     2881 cggggaccgc ggccagcggc gccagggcgg ccaggccggc gctgcgccgg cggtcgagcg
     2941 cggcgaagtc gggcagccag ggtcgcgact ggccagccca ggccagcccc acccgcaaac
     3001 cgtcccgcgg cacccgccct gcccaggccg ccacgaggtc ggcggcggga cgcagatacg
     3061 gcgcgttcgg caccgtcgcg gtggtggcgc cgaaggcccg cggcaggctc agcatggggc
     3121 agtggaaatc gtgcgggggc accggaaggt tgccgaccag cagttcggcg atgccgggga
     3181 gcggcgcgaa caggcgttcc agcggggccg gaacccagac caggacgcgg gcgccgtgct
     3241 gcgccagcag cggcacgtat cgggcaaact ggatcgtgtc gccgaacccc tcctcatgcg
     3301 tcaccagcac gctgcgccct tccaacgaca cggtcggcga cagcgtcggc aacaaggggc
     3361 cgaggcctgc cagggcatgc cgccccggca gcagcaaccg gtattcgtag tcggtccagg
     3421 cctccggcca gcatccggcg tgcagcagtg ccaccgcgcg gttcacgcgc agccgcggat
     3481 tgtccggatc gcgggccacc gcctcggcat aggcgttcag ggccggtcgg aagtccccca
     3541 cggtcttcag cagcatgccg agattggccc agcaggccgc ctgacccggc gccaggctga
     3601 ccgcgcattg cagatgatcg agcgcggcgc cgaaccgtcc caacgcgttg agggcgatgc
     3661 cggccatggt atgggccgcc gcggcgttgg gttgccgctc aagccaactg tgcagcaccg
     3721 gcagcgcctg ctctgcctgg tcgccgtcca gcaggaagcc ggcataggct tcgcagagac
     3781 ggaggtcatc gggggccagg cgtagcgagg cccgaaactg ctccacgatg cgggaccggg
     3841 gcactttcgg cagcgcgcgc gccaggccgg cgcagggatg cggctggtcc ggccgctcgc
     3901 gcgcgacacg gtccaggatg gcggcggcgc gtgcccgctc cccccgcgcc acggcgccat
     3961 agccaagctg cagcagcgcc tccatcgctt ccccggcggg ggcggacggc gccaggccag
     4021 gctgcgcgat ggtccggtca cgcggtgggt cgatggcccg catggtgccg ctctccgttt
     4081 gcattcgggc gaagagttgc ggaaaccgct taagtcccgg ttaacaggaa caccgaaggc
     4141 gacccgctgc ggttcgccct ggtccattgc ctcacgccgc agcagagttg ctgcttgggc
     4201 tggagtcctg ccgatgtcgc tgttcctgcg ccttgccccc ggactgttcg gcctgctcgc
     4261 attggcggcg tgggaggcgg cggtgcgaat cactcatgtg ccggtctacc tggtgccagg
     4321 ccccatcgcg atcgtgggcg ctttcctcgc cgatccggcc gggttgctga tgtcgctgct
     4381 gtccacgctg accgtcacct tcgccgcgct gctggtggcg gcgacgctgg gttcggcgat
     4441 ggcggtagcc atggcgctca gccggctggc gcaggcggcg atccaaccct gggcggtggt
     4501 gctgcaggtc acgccgattg tcgccatcgc cccgctgatc atcgtctggg tgggcgaccc
     4561 gttcacctcg ctggtggtct gcgcgacgat cgtggcgttc ttcccggtat tcgccaacac
     4621 ggcggccggg ctttcctcga cggcggcgga gctgtccgac ctgttccggc tgtatggcgc
     4681 ggggcgctgg aagacgctct ggctgctgcg cctgccggcg gcgctgccgt atttcctggc
     4741 cgggttgcgc atctccggcg ggctggcgct ggtcggcgcg gtggtcgcgg aattcgtcgc
     4801 cggttcgggc ggcttcgcct ccggcctggc ctaccgcatc ctggaggcgg gttatcggct
     4861 ggaggtcgcc cgcatgttcg ccgcgctggt cctgctgtcc ctcgccggca tcgccatcaa
     4921 tgccgcgctt ggcgcgttcg gcagggccat catgcgccgg cgcggcgagg cgtgaggtct
     4981 acaggcccgg aaccggcacc ccgaaggtct ggagcagcag cacgaggttg agcaggagca
     5041 ccaccagcgt ggcggcgcag gccgcggcga aggcgatcgg gccggtgacg aactccccca
     5101 tcaccgcgcg gcggcggctg agcaccagca tggcgatgac cggcaccggg agcacgaggc
     5161 tcagcaccac ctggctgatc accagcgaca tcgtggcatt gtagcccatc gccacgacga
     5221 agaagggtgg cgccatggtg atcagccggc gcagccagag cgggatgcgg aagccgacga
     5281 agccctgcat gatgatctgg cccgccatgg tgccgaccgt cgagctggaa atgccggacg
     5341 ccatcagcga gaccaggaag gccgccgcgg cacccgcgcc cagcagcgga cccaggatgt
     5401 ggtacgccga gccgatctcg gccacgtcgg gatgaccggc atggaacgcc gccgcggcca
     5461 tcatcaccat ggccatgttg acgatcccgg cgaaggtgag ggcaaccacc acctcccggt
     5521 tggagaagcg cagcagctgc cgccgttccg cgttggtgcg cgccgggacg cggtactggg
     5581 tcagcgccga gtgcaggtag atcgcgtgcg gcatcacggt cgcgcccatg atgccgaccg
     5641 ccagggtcgc cgcggcgcca tcgggcaaat gcggcagcac gctgtgccga gcggctgcgg
     5701 cccagtccgg cgggacgatc agcaattcga cgatgtagca gatgccgatc gtcagtacca
     5761 ggccaccgat cgccagctcg atcgggcgga agccgcgatg ctccagcatc agcaggccgt
     5821 aggtgaccgc gccggtgatg cccatgcccc acagcagcgg gatatgcagc agcagtgaga
     5881 ggccgatggc gccgccgagg aactcggcaa ggtcggtggc catcgcggcg atctcgctgg
     5941 ccacccacat gctgatgacc accggcggcc ggaagtggat ccggcagagt tccgccagat
     6001 tgtggctggt gacgatgccc agtttggccg acatggcctg gaacagcatc gccaccaggt
     6061 tggcgaccac caccacccag agcaggccat agccataggc ggagccggcc tggatgttgg
     6121 tcgcgaaatt cccgggatcc atgtaggcga ccgaggcgat gacggccggt ccgctcaccg
     6181 cgatcgcggt gcgtagcccg ccgcgctgcc ccgccagtgc atcgcgcacg gcccgcgtgg
     6241 tgcggtcgga cagcgtcgtc ctggcaacct cgctcatggg ggacccctca ggcggctttc
     6301 cggcaaatat agccatggct acgttatggg caagatgctg tgattgcggg tccgggctgg
     6361 aggatgcggc agtggcatca aatgcgtctg acagttgcat ttgtgccccc cagggccgat
     6421 agaaaggctg gccggcgcgc tgcgggacga ttcggccccg cgcgccggac cggttgatgc
     6481 gggcggcatg cgcggcacgc cgcggcgagc aaaggaatgt gacgtgtcag gcacgtcgga
     6541 cctgccccgg ctgcaggtcc ccatcgaact tccggaagag cccacccagg ccctgcgctt
     6601 cggcaagacg cgcaatgagc ggtccaccgc gctgcttgag gactatgtcg agctgatcgc
     6661 cgatctgctg gccagcaacg gcgaggcccg caccatcgac atcgcccgcc ggcttggcgt
     6721 cacgcacacc acggcggtga agtcgctggc ccgcctgaag cgcgaggggc tggcgacctc
     6781 ccggccgtat cgcggggtgt tcctcaccga agccgggcgg gtgctggccg aacgcgtgcg
     6841 cgcacgccac cggctggtcg tcgacctcct ggtggccgtg ggggtaccgc ccgaagcggc
     6901 cgagt
//
LOCUS       DASSZH010000389         3200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1082724, whole genome shotgun sequence.
ACCESSION   DASSZH010000389 DASSZH010000000
VERSION     DASSZH010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082724"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1313
                     /gene="oxlT"
                     /locus_tag="VFN42_08510"
     CDS             <1..1313
                     /gene="oxlT"
                     /locus_tag="VFN42_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HET8996696.1"
                     /translation="PKASPGFRWLQLVLGIVCMASVANLQYGWTLFVHPIQQKFGWSH
                     AEIQVSFTLFVLLETWLVPFEGAIVDRIGPRWVIMLGGVLTAVAWSIDSAASHLWVLY
                     LGGAVAGIAGGSVYGTCVGNALKWFPDKRGLCAGLTAMGFGAGAAVTIAPIRHMIASS
                     GFETTFLTFGLIYGVIIVICALFVRAPHAHEAPPVQTSRLPQSTVDVAPMDALRSPIF
                     WVMYLMFFLVAAGGLMSAAQLAPIARERHLTDATLVIFGGASALIVALTAHSITNGIG
                     RPINGWISDRIGRANMMGLAFAIEAVAIALFGFVGYSPVAFVVTDALVFFFWGDIFSL
                     FAATVGDCFGRKHATTNYGMMYTAKGVASLLVPLGNVLTHATGSWVAVYAVGCVMNLT
                     AAALGFLVLRPVINARIHGQRTPELGNDFAAAGFARGAGGPAPD"
     gene            1409..2257
                     /locus_tag="VFN42_08515"
     CDS             1409..2257
                     /locus_tag="VFN42_08515"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF011202.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HET8996697.1"
                     /translation="MPTEPAPAKINLYLHVVGRRGDGYHLLDSLVGFAEAGDVLHATP
                     ADDLRLQVTGRFGAALATEPDNLVLRAARALASAAGVLPCAALLLEKHLPVASGIGGG
                     SADAAAALRVLQRLWNHALPADAQQRIALALGADVPVCLHSRTQRMGGIGDRLDAAPR
                     LPECGLLLVNPGVAVETRAVFQARSGAFSPPAVLPSAWADAADLAGFLQATRNDLQVP
                     ALRLQPVIGEVLAAIAAQPDCLLARMSGSGATCFGLFASRSAAERAGVVLRRTNWWCS
                     PGAFRR"
     gene            complement(2431..3108)
                     /locus_tag="VFN42_08520"
     CDS             complement(2431..3108)
                     /locus_tag="VFN42_08520"
                     /inference="COORDINATES: protein motif:HMM:NF018745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HET8996698.1"
                     /translation="MHRTAPIRRIGRIVSGRPRLFIGAAGGLVSLPVLPAAFSETTRA
                     ILAWDVGVLIYLALSALLFTRERSDRMEADAEAQEEGEWTMFALTMAAVVFSFAAILG
                     EFAGTKDMPPTSRTLHIALVAVTLFISWLMTHVTFAFRYAHEYYARDVDAARIDGGLE
                     FPGSDRPDYLDFVYFSLVLGMTFQVSDVQIKARKLRRLATLHGLLSFLFNTIILALTV
                     NIAAGLV"
BASE COUNT          469 a         1058 c         1105 g          568 t
ORIGIN      
        1 taccgaaggc atcgccaggt ttccgctggc tgcagctggt gctgggcatc gtgtgcatgg
       61 cctcggtggc gaacctgcaa tatggctgga cgctgttcgt ccatccgatc cagcagaagt
      121 tcggctggtc gcatgctgaa atccaggttt ccttcactct gttcgtgctg ctcgaaacct
      181 ggctggtgcc gttcgagggc gccatcgtgg accgcatcgg tccgcgttgg gtcatcatgc
      241 ttggcggcgt gctgactgcc gttgcgtggt cgattgattc cgccgcgtcg catctctggg
      301 tgctgtatct tggcggtgcc gtggcaggca ttgccggtgg ttcggtgtac ggcacctgtg
      361 tcggcaatgc gctgaaatgg ttccccgaca agcgcgggct ctgcgccggc ctgaccgcca
      421 tgggattcgg tgccggtgcc gccgtcacca tcgcgccgat ccggcacatg atcgccagca
      481 gcggcttcga gaccaccttc ctgaccttcg gcctgatcta cggcgtgatc atcgtgatct
      541 gcgccttgtt cgtgcgcgcc ccgcatgcgc atgaggcgcc gccggtgcag accagccgcc
      601 tgccgcagag cacggtggac gtggcgccga tggacgcgct gcgctcgccg atcttctggg
      661 tgatgtacct gatgttcttc ctggtcgccg caggcggcct gatgtcggcg gcgcagctgg
      721 cgccgatcgc gcgcgagcgg catttgaccg acgccacgct cgtgatcttc ggcggcgcct
      781 ccgcgcttat cgtcgcgctg acggcgcaca gcatcaccaa cggcatcggc agacccatca
      841 acggatggat ttccgatcgg atcggcagag ccaacatgat gggccttgcc ttcgccattg
      901 aggcggtggc catcgcgttg ttcggctttg tcggctacag cccggttgcc ttcgtcgtca
      961 ccgacgcgct ggtgttcttc ttctggggcg acatcttcag cctgttcgcg gcgaccgtgg
     1021 gcgattgttt cggacgcaag catgccacca ccaactacgg catgatgtac acggcgaaag
     1081 gcgtcgcgtc gctgctggta cccttgggca acgtgctgac ccatgcgacc ggcagctggg
     1141 tggcggtgta tgcggtgggg tgcgtcatga acctcaccgc cgccgccctc ggcttcctgg
     1201 tgctgcgtcc ggtgatcaac gcccggatac acgggcagcg gacaccggaa ctgggcaacg
     1261 acttcgctgc cgccggattt gcgcgcggcg cgggcggtcc agcccccgac tgacgtcgat
     1321 ccaggccgag cgaccgcgcg ccggcggacc caagcaggtg tcctggacat cttgcggcgg
     1381 ggctgccggc gcgctctttt gccccgccat gccaaccgaa cccgctcccg ccaagatcaa
     1441 cctgtatctg catgtcgtcg gccggcgcgg cgacggctac cacctgctcg acagtctggt
     1501 tggctttgcc gaggccggcg acgtgctgca tgccacgccg gccgatgatc tgcggttgca
     1561 ggtgaccggc cgcttcggcg ccgcactcgc caccgaaccg gacaatctcg tgctgcgggc
     1621 ggcgcgcgcg ctcgcgtccg ccgccggcgt gctgccctgc gccgctctgc tgctggagaa
     1681 gcatctgccg gtcgcgtccg ggatcggcgg cggttccgcc gatgcggcgg cggcactgcg
     1741 cgtgctgcag cgtctgtgga accatgccct gcccgcggat gcgcagcagc ggatcgcgct
     1801 cgccttgggc gccgacgtgc cggtgtgcct gcacagccgc acgcagcgca tgggcggcat
     1861 cggcgaccgg ctggacgcgg caccgcgcct gccggagtgc ggcttgctac tggtcaatcc
     1921 cggcgtggcg gtggagaccc gcgcggtgtt tcaggcccgc agcggtgcgt tcagcccccc
     1981 ggcggtactg ccctccgcct gggcggatgc ggcggacctg gcggggttcc tgcaggcaac
     2041 ccgcaacgat ctgcaagtgc cggcgctgcg gctgcagccg gtgatcgggg aggtgctggc
     2101 ggcgatcgcg gcgcagcccg actgcctgct ggcgcgcatg agcggctccg gcgccacctg
     2161 cttcggcctg ttcgcttcac ggtcggccgc ggaacgcgcc ggggtcgtgc tgcggcgcac
     2221 gaactggtgg tgctctccgg gagcatttcg ccgctagccg cgctgggcgc cagcgcctcg
     2281 ccgtccatcg cgggaatcgt tgcgccgcca gtcatgctgg ccagcgcgaa agcggctgtc
     2341 cggcccaggg cgcgctacag ccgcgggcga gtccatcagg ctgtggcatg caggcgaccg
     2401 ggccgggcgg tccctgtcgt cgcggcttcg ctacaccagt cccgccgcga tgttcacagt
     2461 cagcgcgagg atgatggtgt tgaacagaaa actgagcagc ccatgcagcg tcgcgagtcg
     2521 ccgcagcttg cgtgctttga tctgcacgtc ggacacttgg aacgtcatgc ccagaaccag
     2581 ggagaaatag acgaagtcga gatagtccgg ccggtctgaa cccgggaatt ccagcccgcc
     2641 gtcaatccgg gccgcatcga cgtcgcgcgc gtaatactcg tgcgcatagc ggaatgcgaa
     2701 cgtgacatgc gtcatcagcc aggagatgaa cagggtgacg gccaccaggg cgatatgcag
     2761 cgtgcggctg gtcggcggca tgtccttggt gccggcaaat tcgcccagaa tggcggcaaa
     2821 gctgaacacc accgctgcca tggtcagcgc aaacatggtc cactcgcctt cctcctgcgc
     2881 ttcggcatcg gcttccatgc ggtcggatcg ctcgcgcgtg aacagcaggg cggaaagcgc
     2941 gagatagatc agcacgccaa cgtcccaggc gagaatcgca cgggtggttt cgctgaaggc
     3001 cgcaggtagc actgggaggg agaccagtcc gccggcggcg ccgatgaaca gccgcggccg
     3061 gccggagacg atacggccga tccggcggat gggagcggtg cgatgcatgg gcctggatcc
     3121 tcggaacggc aaataatgag ccgggcagcc gaacaaggga acgcgtaggc ctgcttgcga
     3181 ttgactttcc cgtgcgacgc
//
LOCUS       DASSZH010000390         2199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_571601, whole genome shotgun sequence.
ACCESSION   DASSZH010000390 DASSZH010000000
VERSION     DASSZH010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571601"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /locus_tag="VFN42_08525"
     CDS             <1..1305
                     /locus_tag="VFN42_08525"
                     /inference="COORDINATES: protein motif:HMM:NF019291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HET8996699.1"
                     /translation="AGAFLLPAVQRMQAAVPDVEPAIALANLSSRLRGWEIDPFAAWL
                     AQLSVETALLPLVVAAGRRLGPVVEVTDALAAFPGAADSWDLVMGNPPFGKVKDTPAL
                     RARFRRSLYGHPNWYGLFVDLAVHLARPEGGVVAYLTPASFLAGQYFRNLRRLLHEQA
                     PPVSLDLVESRADVFEDVLQEVTLSCFRRGAALRPAPCAVVHVTQAGLKVQPTGTLVL
                     PNVADAPWTLPRSPADTGLVARLHAMPVRLADWGYAVSTGPLVWNRHKPRLHDTPKRG
                     RVPVVWAESVTADGRFVLKASKRNHRAWFQPRGADDPNLVDRPCVLVQRTTAKEQNRR
                     LIAAEMPASLIKQHGVVAVENHLNMIRPLGPRPKVPLRVLAAFLATPTADRVLRCINA
                     SVAVSASELEAMPLPRAEQVIAAFRQPGFAGAVSRLYGGAPG"
     gene            1302..>2199
                     /locus_tag="VFN42_08530"
     CDS             1302..>2199
                     /locus_tag="VFN42_08530"
                     /inference="COORDINATES: protein motif:HMM:NF037617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HET8996700.1"
                     /translation="MSLPALLPWQAIRSRLQVVFPEGTPDRHFLIREASARTVFVALY
                     IGAVQGLDRWLAPRHVVRMSDAQAALSDDASRLAYHAAMNGARAPVPVERWYADNTRE
                     PLRDEVIRQGLVPVNAIIERGGLPTTSPLGRYALQAEFAALFDPALDDLGFEQAAVAW
                     RACNLSPAALARAALVRSSASTATGNISVQFPNGPAIVLPPGPSQQITKAVVEIFAPA
                     FLVDPRVAWVSDSASKRPFRDAPLEHALQIALDAAELLPDIVLVDLAPPGRPGGVLIV
                     FVEVVASDGPVTEQRRRALLALL"
BASE COUNT          287 a          777 c          780 g          355 t
ORIGIN      
        1 gccggggcct tcctgctgcc ggcggtgcag cggatgcagg cggcggtgcc ggatgtcgaa
       61 ccggccatcg cgctggccaa cctgtcctcc cgcctgcgcg gctgggagat cgacccgttt
      121 gccgcctggc tggcgcaact ctcggtcgaa acggcgctgc tgccactggt ggtcgccgct
      181 ggccgccgac tcgggccggt ggtggaggtt accgatgccc tcgcggcgtt tcccggcgcg
      241 gcggatagct gggacctcgt gatgggcaat ccgcccttcg gcaaggtgaa ggacacgccg
      301 gccctgcgcg cccgcttccg ccgcagcctg tacggccatc ccaactggta cgggctgttc
      361 gtcgacctgg cggtgcacct ggcgcggccg gagggcggcg tggtcgccta tctgaccccg
      421 gccagttttc tggccggcca gtacttccgc aacctgcgcc gcctgctgca tgagcaggcg
      481 ccgccggtct cgctggacct ggtggaaagc cgcgccgacg tgttcgagga cgtgctgcag
      541 gaggtgaccc tctcctgctt ccgccgcggc gccgccctga ggccggcgcc ctgcgcggtg
      601 gtgcacgtga cccaagccgg cctgaaggtg caacccaccg gaacgctggt gctgcccaac
      661 gtggcggatg cgccctggac cttgccgcgc tccccggccg ataccgggct ggtggcgcgg
      721 ctgcatgcca tgccggtacg gctggcggac tggggctatg cggtttccac tggcccgctg
      781 gtctggaacc gccacaagcc ccggctgcac gacacaccca aacgcggacg cgtgccggtg
      841 gtctgggcgg aatcggtcac cgccgacggc cgcttcgtgc tgaaggccag caagcggaat
      901 catcgtgcct ggttccagcc gcgcggtgcc gacgacccaa acctggtgga ccgcccctgc
      961 gtgctggtgc agcggaccac cgccaaggag cagaatcgcc ggctgatcgc cgcggaaatg
     1021 ccggccagcc tgatcaagca gcacggcgtg gtcgcggtgg agaaccacct gaacatgatc
     1081 cgcccgctgg ggccccgccc gaaagtgccg ttgcgcgtgc tggcggcgtt cctcgccact
     1141 ccgaccgccg accgggtgct gcgctgcatc aacgccagcg tggcagtcag cgcgtcggaa
     1201 ctggaagcca tgccgctgcc gcgcgcggag caggtcatcg ccgcgtttcg ccagcccggg
     1261 tttgccggtg cggtcagccg tctgtatggc ggtgcccccg gatgagcctg ccggcgctgc
     1321 tgccctggca ggcgatccgc tcccggcttc aggtggtctt cccggaaggc acaccggacc
     1381 ggcatttcct gatacgcgag gcctcggccc gcaccgtgtt cgtcgccctg tatatcgggg
     1441 cggtgcaggg cctggaccgc tggctggcgc cgcgccacgt ggtgcgcatg agcgacgcgc
     1501 aggccgcgct atcggacgat gcatcccggc tggcgtatca cgcggcgatg aacggcgccc
     1561 gtgcgccggt gccggtggag cgctggtatg ccgacaacac ccgcgagcca ttgcgcgacg
     1621 aggtgatccg ccaggggctg gtgccggtca acgcgatcat cgaacgcggc ggcctgccga
     1681 ccacgtcgcc gcttggccgt tatgccctgc aggctgagtt cgcggcgctg ttcgacccgg
     1741 cattggacga tctgggcttc gaacaggccg ccgtggcctg gcgcgcatgc aacctgtctc
     1801 ctgccgcgct ggcccgcgcc gcgctggtgc gctccagcgc cagcacggcg accggcaaca
     1861 tcagcgtcca gttccccaac ggtcccgcga ttgtgctgcc gcccggaccc agtcagcaaa
     1921 tcaccaaggc ggtggttgag atttttgcgc ccgccttcct ggtcgatccg cgcgtcgcct
     1981 gggtgagcga cagcgcgagc aagcggccgt tccgcgatgc gccgctggaa catgcgctgc
     2041 agatcgcgct ggatgccgct gaactgttgc cggacattgt gctggtcgat ctggcgccgc
     2101 ccggcaggcc cggcggcgtg ctgatcgtgt tcgtggaagt cgtggcgtcc gatggcccgg
     2161 tgacggaaca gcggcgacgc gcgctgctcg cgttgctgg
//
LOCUS       DASSZH010000391         3463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1679851, whole genome shotgun sequence.
ACCESSION   DASSZH010000391 DASSZH010000000
VERSION     DASSZH010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1679851"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            346..597
                     /locus_tag="VFN42_08535"
     CDS             346..597
                     /locus_tag="VFN42_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HET8996701.1"
                     /translation="MRAIFVMIKCEMGQAYKVAQEAADTIEQMSELHSTSGQYDLLGK
                     FYLDPEQDIGLFVTEKIQTLPGVKDTYTLITFNAFTAAR"
     gene            complement(681..902)
                     /locus_tag="VFN42_08540"
     CDS             complement(681..902)
                     /locus_tag="VFN42_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996702.1"
                     /translation="MLAMFSFRRRKEPPFKRDRLGPQQLESIFPEFEVTVPMPRSTKI
                     PPKVIVIPAPEEPEDSAAVSSTPRSSSAS"
     gene            complement(877..1329)
                     /locus_tag="VFN42_08545"
     CDS             complement(877..1329)
                     /locus_tag="VFN42_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996703.1"
                     /translation="MYRDQLARRVRFVELVKAIGSGGAIAGWVVWRDWPLLWSGIIAA
                     AQFLDATKQVLPFARRHGAASELTLALELLCIDAEAEWESVHPGKIPDEGIVERRTRL
                     ARLRAEAEHKYFPEGFAPGRRLIAVATRQANDYIRATYGEDAGHVLFP"
     gene            complement(1462..2607)
                     /locus_tag="VFN42_08550"
     CDS             complement(1462..2607)
                     /locus_tag="VFN42_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET8996704.1"
                     /translation="MASSSDRPVVLQVLPSLVTGGVERGTVEITQAVAQADGVALVAS
                     AGGPMVGAVQRAGGRHITLPLHRKDPLSLWRNAARLAALIRAERVSLVHARSRAPAWS
                     AWLACRRTGAHFVTTYHGTYTETLPLKRQYNAIMARGEVVIAASRFIAELIVARHSVY
                     PTRIRVIPRGVDPNVFNPDSVSAERLVRLERAWRLPEGAPVIMLPGRLTGWKGQGVLI
                     DALAQLERKDVWGVLVGADQGRRRYAMSLMRQAERLGVADRLRLVGQCDDMPAALMLA
                     DVVVHASTQAEAFGRVVIEAQAMARPVIAADLGGPVETVQHGVTGWRVPPGDPAALAA
                     AIQHVLTLPPGERQAMGARARAGVPTVQAMQDATLQVYEAVLGGAAD"
     gene            2741..>3463
                     /locus_tag="VFN42_08555"
     CDS             2741..>3463
                     /locus_tag="VFN42_08555"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET8996705.1"
                     /translation="MKLFDLNGKVALVTGGNGGIGLGMAQGMASCGASIMITGRNAAK
                     AEPALAALRALGVAAEFLAADVTRKAECVKLVADTVARFGRLDILVNNAGTTIRKMPQ
                     DYTEAEWHEVLDTNLTSAFLCSQAAYAPMQAAGGGKMINIGSMMSLFGAPYAVPYASS
                     KGGIVQMTRALATAWAKDNIQVNAVLPGWIDTDLTRRAREQVAGLQERVEARTPSGRW
                     GTPQDMAGIAAFLASPASDFVTG"
BASE COUNT          580 a         1248 c         1122 g          513 t
ORIGIN      
        1 ctgcagcacc agccgggaca gatcacgctt gacgtcacgc gggggacggt gggcggcggc
       61 attcccggtc gcgccggctc tcgccgaata cccaatcccc cgaccgagtg gcggtggcgg
      121 acaaactgtg attgtgtcct gtgcgaccgc ttcggcaggt cttggattgg aaccatgttg
      181 tctcatgcct tgagccatga acgcacgcgt ccagggttgc cggctggtct gtgtgtgcat
      241 ggccagccag gcggccgttg ctcgtgaggc atctgctgcg ctggcggatt ccggacggaa
      301 gcttggcgct gtccggataa gagcggcctg aaatcaggag gttagatgcg agcgatcttc
      361 gtcatgatca agtgcgagat gggtcaggcc tacaaggtgg cccaggaggc cgccgacacg
      421 attgagcaga tgtcggaact gcactccaca tccgggcagt acgacctgct gggcaaattc
      481 tatctggacc ccgagcagga catcgggctg ttcgtcaccg agaagatcca gacgctgccg
      541 ggggtgaagg acacctatac cctgatcacc ttcaacgcgt tcaccgccgc gcggtgagtg
      601 ccgggcattc gcggcgcctt gcggggattt cggccgccca gtcggggcgg catgtcgcct
      661 taccggccgg ctgcggcgca tcacgaagcc gaagacgacc tcggcgtcga tgatacggcc
      721 gccgaatcct ccggttcctc cggcgcagga atgacgatca ccttcggcgg tatcttggtg
      781 ctgcgcggca tcggcacggt gacctcgaat tcgggaaaga tgctctcaag ttgctgcggc
      841 cccaaacgat cacgcttgaa gggcggctcc ttgcgcctac ggaaagagaa catggccagc
      901 atcctcgcca taagtcgccc gaatgtagtc gttcgcctgc cgcgtcgcca cagcgatcaa
      961 gcgccggccg ggcgcaaagc cctcggggaa gtacttgtgc tccgcctcag cgcgcaaccg
     1021 tgccagccgc gtgcggcgtt caacaatgcc ttcgtcggga atttttcccg gatgcacgct
     1081 ctcccattcg gcctctgcgt cgatgcacag caactccaac gccaacgtca actcgcttgc
     1141 ggcaccgtgc cggcgtgcga agggaagcac ctgcttggtt gcatccagaa actgcgcggc
     1201 ggcaatgatc ccagaccaca acagcggcca atcccgccat accacccagc cggcgatggc
     1261 tccgccggag ccgattgcct ttaccagctc cacgaagcgc acgcggcgcg ccaactggtc
     1321 gcgatacaac agtatgcaga ttgctgccgc tttcaggcgg accaattgct tccagtagag
     1381 atcgggctgc cgccgcgggt ttgctgcatc ggacacgcga cggaggcata ggccagccgc
     1441 atgaaggaat gattgacgcg gctaatccgc tgccccgccc agcaccgcct catatacctg
     1501 cagcgtggca tcctgcatcg cctgcaccgt cggcacgccg gcgcgcgcgc gcgcgcccat
     1561 cgcctggcgc tcccccggcg gcagggtcag cacgtgctgg atcgccgccg ccagcgcggc
     1621 ggggtcgccg ggcggcaccc gccagccggt caccccgtgc tgcaccgtct ccaccggacc
     1681 gcccagatcc gcggcgatca ccggccgcgc catcgcctgc gcctcgatca ccacccggcc
     1741 gaacgcttcc gcctgggtag aggcgtgcac cacgacgtcc gccagcatca gcgccgcagg
     1801 catgtcgtcg cattgcccga ccaaccgcag ccggtccgcc accccaagcc gctcggcctg
     1861 gcgcatcagg gacatggcgt agcgccggcg cccctggtcg gcaccgacca gcacgcccca
     1921 cacatccttc cgctccagtt gcgccagggc atcgatcagc accccctggc ccttccagcc
     1981 ggtcagccgc cccggcagca tgatcaccgg cgcgccttcc ggcagccgcc atgcgcgctc
     2041 cagccgcacc agccgttccg ccgatacgct gtccggattg aacacgttgg gatccacgcc
     2101 gcgcgggatc acccggatac gtgtcggata aacgctatgg cgtgcgacga tcagctcggc
     2161 gatgaaccgg ctggccgcga tcaccacctc gccgcgtgcc atgatcgcgt tgtactggcg
     2221 cttgagcggc agcgtctcgg tgtaagtgcc gtgatacgtg gtgacgaaat gcgcgccggt
     2281 acggcggcag gccagccagg ccgaccaggc gggcgcccgc gaccgcgcat gcaccaggct
     2341 gacccgctcc gcccggatca gcgccgccag ccgagcggca ttgcgccaca gggagagcgg
     2401 atccttgcga tgcagcggca gggtgatgtg ccggccaccg gcgcgctgca ccgcgccgac
     2461 catcggcccg ccggcgctcg ccaccagcgc caccccgtcc gcctgggcca cggcctgggt
     2521 gatctccacg gtgccgcgct ccaccccgcc ggtgaccagc gaaggcagca cctgcaacac
     2581 caccggccgg tcggaggagg acgccatgtc cggcttctac acgcgggcgc cgacggacgt
     2641 cacccctggg aaccggcctc ccgcccgcgc caccggccgg cgcccgattg cccagcctct
     2701 cccagccgtg ccagtttccg cccgcgaagg gagtccgcgg atgaaactgt tcgacctcaa
     2761 cggcaaggtg gcgctggtca ccggcggcaa tggcggcatc ggcctgggca tggcgcaggg
     2821 catggcgtcc tgcggcgcca gcatcatgat caccggccgc aacgccgcga aggcagagcc
     2881 cgccctggcc gcgctgcgcg ccctgggcgt ggcggcggag ttcctggctg ccgacgtcac
     2941 ccgcaaggcc gagtgcgtca agctggtggc ggacaccgtg gcccggttcg gccggctgga
     3001 catcctggtg aacaacgccg gcaccacgat ccgcaagatg ccgcaggact acacggaagc
     3061 cgagtggcac gaggtgctgg acaccaacct gaccagcgcc ttcctgtgct cccaggccgc
     3121 ctatgcgccg atgcaggccg ccggcggcgg caagatgatc aacatcggct ccatgatgtc
     3181 gctgttcggc gcgccgtacg cggtgcccta tgcctccagc aagggcggca tcgtgcagat
     3241 gacgcgggca ttggccaccg cctgggcgaa ggacaacatc caggtcaacg cggtgctgcc
     3301 cggctggatc gacaccgacc tgaccaggcg ggcacgcgag caggttgccg gcctgcagga
     3361 acgggtggaa gcgcgcacgc ccagcggccg ctggggcacg ccgcaggaca tggccgggat
     3421 cgccgccttc ctcgccagcc cggcgtcgga cttcgtcacc ggc
//
LOCUS       DASSZH010000392         2464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_605987, whole genome shotgun sequence.
ACCESSION   DASSZH010000392 DASSZH010000000
VERSION     DASSZH010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605987"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1622)
                     /locus_tag="VFN42_08560"
     CDS             complement(<1..1622)
                     /locus_tag="VFN42_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET8996706.1"
                     /translation="MIAAIIRWSAANRFMVLLGTLFIVAAGIWAVRNTPLDAIPDLSD
                     VQVIVYTDYPGQAPQVVEDQVTYPLTTALLTVPKAKTVRGFSFFGSSFVYVVFEDGTE
                     LYWARSRVLEYLSQAAQRLPSDVTPALGPDATGVGWVFQYVVTGAQRTLAELRSIQDW
                     FLRYQLSRGEGVAEVASLGGFVQQYQVVVDPQKLQAYGIPLSKVTEAIRASNRDVGGR
                     SIEMSETEYLVRGRGYLRGIGDIEQVVLAVQNGVPVRVGDVARVELGPDERRGISELN
                     GEGEVVSGIVLQREGQNALSVIRSVKQRLVEIAGSLPEGVKVSAVYDRSALIYRAIDT
                     LKHTLLEESAIVALVCIVFLLHVRSALVAIVMLPVGVLIAMLLLHLLGLTSNIMSLGG
                     IAIALGAMVDAAIVMIENAHKRLERGDIATPTSWRAPARHPRLSSGAKEEVVDGGPSP
                     AMTGRVKILTDAAIEVGPALFFSLAVITVSFLPVFTLEDQEGRMFRPLALTKTFAMGG
                     GALLSITLVPVLMLLFIRGRILPERRNPVNRLL"
     gene            complement(1619..>2464)
                     /locus_tag="VFN42_08565"
     CDS             complement(1619..>2464)
                     /locus_tag="VFN42_08565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET8996707.1"
                     /translation="WIERLRVNQTGESVRRGQKLFEMYSPELVATAQEAAIARRAHAE
                     MGEADPTVRSAAAGIAASAAARLRNFGITASGRNFALTAPIDGVVLEKTAVQGMRFAP
                     GDPLYRIADLSTIWVIANVFERDLGQVQPGESASVVLTAYPGRHFSGRVTFIYPTLNA
                     ATRTGRVRIELANPELLLKPDMYATVEIAAGMSGSKVTAVPDSAVLDSGTRQIALVER
                     GIGRYQPRPVTTGTRAGGFIEIRSGLTAGERVVVGANFLIDAESNLRAALQAFTADQP
                     QGQSK"
BASE COUNT          414 a          834 c          827 g          389 t
ORIGIN      
        1 agcagccggt tcaccggatt gcgccgctcc ggcaggatgc ggccgcggat gaacagcagc
       61 atcagcacgg ggaccagcgt tatcgacagc agcgcgccgc cgcccatggc gaaggtcttg
      121 gtaagggcca gtgggcggaa cattcggcct tcctgatcct ccagcgtgaa caccggcagg
      181 aaggacacgg tgatcaccgc caggctgaag aacagcgccg gaccgacttc gatcgccgca
      241 tcggtcagaa tcttcacgcg tcccgtcatg gcgggcgaag gcccgccatc cacgacttct
      301 tctttcgcgc ccgaggaaag tcgtgggtgg cgggccggcg cccgccacga cgtgggtgtc
      361 gcgatgtctc cgcgctccag ccgcttgtgc gcattctcga tcatgacgat ggcagcatcc
      421 accatggcgc cgagggcgat ggcgatgccg cccaggctca tgatgttgga ggtgaggccg
      481 agcaggtgca gcagcagcat ggcgatcagc acgccgaccg gcagcatgac gatcgccacc
      541 agcgcgctgc gcacgtgcag caggaacacg atgcagacca gcgcgacaat ggcgctttcc
      601 tccagcagcg tgtgcttcag cgtgtcgatc gcgcgataga tcagggcgga gcggtcgtac
      661 accgcggaaa ccttcacacc ctccggcaag ctgccggcga tctccaccag gcgctgcttc
      721 acgctgcgga tcaccgacag cgcgttctgc ccctcgcgct gcaagacgat gccgctgacc
      781 acctcgcctt cgccgttcag ttcggagatg ccgcggcgct catccgggcc gagttccacg
      841 cgcgccacgt cgcccacccg caccggcacg ccgttctgca ctgccagcac gacctgctcg
      901 atgtcgccga tgccgcgcag gtagccacgg ccgcgcacca ggtattcggt ttccgacatc
      961 tcgatcgagc ggccacccac gtcgcggttg ctggcacgga tcgcttcggt gaccttggac
     1021 agcgggatac cgtaggcttg cagcttctgc ggatcgacca ccacttggta ctgctgcacg
     1081 aagccaccga ggctggcgac ttccgccacg ccttcgccac gggaaagctg atagcgcagg
     1141 aaccagtcct ggatgctgcg cagttcggcc agcgtgcgtt gcgccccggt gaccacgtac
     1201 tggaacaccc agcccacgcc ggtggcgtcg gggccgagcg cgggcgtcac gtcggaaggc
     1261 aggcgctgcg cggcctggct gagatattcc agcacccggc tgcgcgccca gtacagctcg
     1321 gtgccgtcct cgaacacgac atagacgaaa gacgagccga agaacgagaa gccgcgcacc
     1381 gtcttcgcct tcggcacggt cagcagggcg gtggtgagcg ggtaggtgac ctggtcctcc
     1441 accacctgcg gcgcctggcc gggatagtcg gtgtagacga tcacctgcac gtcggagagg
     1501 tcgggaatgg catccagcgg cgtgttgcgc accgcccaga tgccggcggc aacgatgaac
     1561 agggtgccca gcagcaccat gaagcggttg gccgccgacc agcggatgat ggcggcgatc
     1621 atttcgactg gccctgcggc tggtcggcgg tgaaagcctg caatgccgcg cgcaggttgc
     1681 tctccgcatc gatcaggaag ttggcgccca ccactaccct ctcgccggca gtaagaccgc
     1741 tgcggatttc gataaagccg ccggcacgcg tgccggtggt gacagggcga ggctgatagc
     1801 ggccgatgcc gcgttcgacc aacgcgatct ggcgcgtgcc gctgtccagc accgcggaat
     1861 ccggcaccgc ggtcaccttg ctgccggaca tgccggcggc gatctccacc gtggcgtaca
     1921 tgtcgggttt cagcagcagt tccgggttgg cgagttcgat gcgcacccgg ccggtgcgcg
     1981 tggcggcatt cagcgtgggg tagatgaagg tgacccggcc gctgaagtgg cgacctgggt
     2041 atgcggtgag caccacgctc gcgctttcgc ccggctgtac ctggccgagg tcacgttcga
     2101 acacgttggc gatcacccag atggtggaca ggtcggcaat gcggtacagc gggtcgcccg
     2161 gcgcgaagcg catgccctgc accgcggtct tctccagcac cacgccatcg atcggcgccg
     2221 tcagggcgaa gttgcggccg gacgcagtga tgccgaagtt gcgcaatcgc gccgcggcgg
     2281 aggcggcaat gccggccgcg gcactgcgca ccgtcgggtc ggcctctccc atctcggcgt
     2341 gggcgcggcg ggcgatcgcg gcttcctgcg cggtggccac cagttcgggg ctgtacatct
     2401 cgaacagttt ctgcccacgg cgcacgctct cgccggtctg gttcacccgc aggcgctcga
     2461 tcca
//
LOCUS       DASSZH010000393         2191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_350088, whole genome shotgun sequence.
ACCESSION   DASSZH010000393 DASSZH010000000
VERSION     DASSZH010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350088"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..885)
                     /gene="uppS"
                     /locus_tag="VFN42_08570"
     CDS             complement(10..885)
                     /gene="uppS"
                     /locus_tag="VFN42_08570"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HET8996708.1"
                     /translation="MLGVDSHAGAAPPIGVGVGLRRRMLRVLWPLLRTAAWPVYAVYE
                     QRLVRQVALRPPPRHIGLVLDGNRRYAMREGLAHAREAYRLGAQKLDQVIEWCLELSI
                     PAVTLWVCSTDNLARPADQVAGLLAAVEEKMRALAKDPHIHSHRIRVRAIGRLEQLPA
                     GTRAAIRAAEQATAGYDGMLLSIAVAYGGREEIADAVRGLLAEAAERGCSLPELAEAV
                     TPEAIARHLYLAAAPEPDLIIRTSGEVRLSGFLLWHSATSEFYFSEVLWPAFRRIDFL
                     RAIRAFQERNRRFGR"
     gene            complement(1015..>2191)
                     /locus_tag="VFN42_08575"
     CDS             complement(1015..>2191)
                     /locus_tag="VFN42_08575"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HET8996709.1"
                     /translation="CLDMNDRALRSIVNSLGGVANGFPREDGFDITVASEVMAVFCLS
                     KSVKELQERLGRMIVAQKRDSSYVTAKDLKADGAMSVLLKDAMMPNLVQTLEGSPAFV
                     HGGPFANIAHGCNSVMATRLGLKLSDVVVTEAGFGADLGAEKFLDIKCRSAGLKPSCA
                     VVVATVRALKMHGGVARANLGKEDVAAVQRGVANLARHVENLGKFGLPVVVGVNHFTS
                     DTEAEFAVIREAMANLGVEAILCTHWADGGAGAEALARAVQQKIVLDEARYAPLYPLE
                     MPFAEKIRTIAQQLYHASDIAMSDAVAKRLKGFEEAGFGHLPVCIAKTQYSFTSDPTV
                     MGAPEGHVLPVREVRLSAGAGFVVAICGDIMTMPGLPRVPSAEAIHLREDGEIEGLF"
BASE COUNT          352 a          827 c          679 g          333 t
ORIGIN      
        1 ccgccgcaac tacctgccga atctccggtt gcgctcctgg aacgcccgga tcgcgcgcag
       61 gaagtcgatg cgccggaagg ccggccacag cacctcggag aagtagaact cgctggtcgc
      121 gctgtgccac agcaggaagc cggaaagccg cacctcgccg ctggtgcgga tgatcaaatc
      181 gggttccggc gccgccgcca ggtacaaatg gcgcgcgatc gcttccggcg tcaccgcctc
      241 ggccagctcc ggcagcgagc agccgcgttc cgccgcctcg gccagcaacc cgcgcaccgc
      301 gtcggcgatc tcctcccgcc cgccatacgc caccgcgatc gacaacagca tgccgtcata
      361 accggcggtt gcctgctccg ccgcgcggat cgccgcccgc gtgccggccg gcaattgctc
      421 cagccggccg atcgcgcgca cccggatgcg atggctatgg atatgcgggt ccttcgccag
      481 cgcccgcatc ttctcctcca ccgcggcgag cagcccggcc acctggtcgg ccgggcgcgc
      541 caggttgtcg gtggagcaca cccacagcgt caccgccgga atcgacagtt ccaggcacca
      601 ctcgatcacc tggtccagct tctgcgcgcc cagccggtag gcttcgcgcg cgtgcgccag
      661 gccttcccgc atcgcgtagc ggcggttgcc gtcgagcacc aggccgatat gccgcggcgg
      721 cgggcgcaac gcgacctgcc ggaccagccg ctgctcgtac acggcgtaaa ccggccacgc
      781 ggccgtgcgc agcaagggcc acagcacccg cagcatgcgg cggcgcaggc caacgccgac
      841 gccgatcggc ggcgcggcgc ccgcgtgaga atcgacaccc agcaattcgt ttcccctccc
      901 cgcggcgaat cactgcccgc gcatatcggg cagcgccctc tcagcgggtc aagtgaagct
      961 tcgccaagcc tgcgatgcgg cgggtttcgg aaccgctgca ggtttgctcg ccgatcagaa
     1021 caatccttcg atttcgccat cctcccgcag gtggatcgcc tcggccgagg gcacccgcgg
     1081 caggcccggc atggtcatga tgtcgccgca gatcgccacc acgaacccgg cgccggcgga
     1141 cagccgcacc tcgcgcaccg gcagcacgtg cccttccggc gcgcccatca ccgtgggatc
     1201 cgacgtgaag ctgtactgcg tcttggcgat gcacaccggc agatggccga acccggcttc
     1261 ctcgaacccc ttcagccgct tggcgaccgc atcgctcatg gcgatatccg acgcgtggta
     1321 gagctgctgc gcgatggtgc ggatcttctc ggcgaacggc atctccagcg gatacagcgg
     1381 cgcatacctc gcttcatcca gcacgatctt ctgctgcacc gcgcgggcca gcgcctcggc
     1441 accggcgccg ccatcggccc agtgcgtgca caggatcgcc tccacgccca gattcgccat
     1501 cgcctccctg atcaccgcga actccgcctc ggtatcggag gtgaagtggt tcacgccgac
     1561 cacgacggga aggccgaatt tgcccaggtt ctccacgtgc cgcgccaggt tggcgacgcc
     1621 gcgctgcacc gccgcgacgt cttccttgcc caggttggcg cgcgccacgc cgccatgcat
     1681 cttcagcgcg cgcaccgtcg ccaccaccac ggcgcaggac ggcttcaacc cggcggaccg
     1741 gcacttgatg tcgaggaact tctccgcgcc gaggtcggcg ccgaacccgg cttccgtcac
     1801 caccacatcg gacagtttca ggccaagccg cgtcgccatc acgctgttgc agccatgcgc
     1861 gatgttggcg aaggggccgc cgtgcacgaa cgcaggcgaa ccttccagcg tctgcaccag
     1921 gttgggcatc atcgcgtcct tcagcagcac ggacatcgcg ccgtccgcct tcaggtcctt
     1981 cgcggtgacg taggagctgt cgcgcttctg cgcgacgatc atgcggccga gccgttcctg
     2041 cagttccttg acgctcttgg acagacagaa gactgccatg acttcggagg cgaccgtgat
     2101 gtcgaacccg tcttctcgcg gaaagccgtt ggcgacgccg cccaggctgt tgacaatgga
     2161 gcgcagcgca cggtcgttca tgtccaggca g
//
LOCUS       DASSZH010000394         4777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_282115, whole genome shotgun sequence.
ACCESSION   DASSZH010000394 DASSZH010000000
VERSION     DASSZH010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282115"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VFN42_08580"
     CDS             <1..326
                     /locus_tag="VFN42_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET8996710.1"
                     /translation="RSHVPGTKAELAIKGEFVPLTQSAAAKTLFELYVSAAADTGFRT
                     EGEFTGGCADSGFTAAVGAPTICAVGPVGGKAHSPDEFLRLDSLVPRAQACARAILRL
                     ERVGL"
     gene            complement(369..650)
                     /locus_tag="VFN42_08585"
     CDS             complement(369..650)
                     /locus_tag="VFN42_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996711.1"
                     /translation="MTRSLRVRSRRFRWLLPVVALGAAAVLGGCVAYPDDYYGYSNYG
                     YGYGYAPAPYYGYNPGYVAFGFGGDHDWGDHDWGDHDWHGGWHHGHDGR"
     gene            complement(853..2124)
                     /gene="glgC"
                     /locus_tag="VFN42_08590"
     CDS             complement(853..2124)
                     /gene="glgC"
                     /locus_tag="VFN42_08590"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HET8996712.1"
                     /translation="MPSAASSLSAPLARSSMAYVLAGGRGSRLMELTDIRAKPAVYFG
                     GKSRIIDFALSNALNSGIRRIGVATQYKAHSLIRHLQRGWNFFRPERNETFDILPASQ
                     RVSETQWYAGTADAVFQNIDIIESYSPDYIVVLAGDHVYKMDYEHMLQQHVEQRADVT
                     VGCFEVPRMDATAFGVMHIDASGRITSFLEKPKDPPGMPDKPDRALASMGIYVFETKF
                     LIDQLCRDATDPNSRHDFGGDIIPHIVKHGTAVAHRFTDSCVRSSAEAEAYWRDVGTL
                     DAYWEANIDLTHPTPSLDLFDRDWPIWTYGEIVPPAKFVHDLEGRRGQAISSLVSGGC
                     IISGASVRQSLLFTGVHVHSYAQIESAVVMPYVSIARSACLRNVIVDRGVAIPPGLVV
                     GEDPELDAKRFRRTERGVCLITQPMIDRLAH"
     gene            complement(2129..3571)
                     /locus_tag="VFN42_08595"
     CDS             complement(2129..3571)
                     /locus_tag="VFN42_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8996713.1"
                     /translation="MTQTTWIRNAAWIVAWDRASGRHAYLRDADLAFSGNAIVHVGKN
                     YAGPADTIIDGRDRLLIPGLVDIHAHPSTEPFFRGIREEHGVPAMYMTGLYERFGAFR
                     TDSDGRRAGKTVAYCEMLRTGITTVADLSPPDPWWIDLAASSGLRVFLAPGFASARWR
                     LENGWQLHYAWDEPAGQRGLQDALALIDRARRHPSGRLSGIVFPGQIDTCSESLLRDS
                     AAAAAENNLPLTTHCAQSVTEFNEMLHRHGKTPISWAHDIGILGPRTILGHAIFTDEH
                     SWTRWHTRDDIRLLAETGASVAHCPSPFARYGQMLEDFGRYRRAGVNVGIGTDVSPHN
                     LIEEMRLAAILARIAARDITATSTEAIFHAATIGGAQALGRDDLGRLAPGARADLVLI
                     DLTNPFMQPVRDPLRSLIYTAADRAVHSVFVDGHRVVEDGRVVTLDHAGALAELAEAQ
                     ARMQDAVPQHDWAHRSADQITPLSLPTLTG"
     gene            complement(3661..4590)
                     /locus_tag="VFN42_08600"
     CDS             complement(3661..4590)
                     /locus_tag="VFN42_08600"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET8996714.1"
                     /translation="MPLSRRYLAREAWLAQSEQMCICCGLVNPRSAEGNLGAELILDA
                     WFRRRTMRQYRKRHVEEITLAVERFTPVLDEARAAIDRGDMARARELWQPVLAACPAI
                     VAASTKAALLAIEIEDSATADSLIRTALQDFPNRVFLWEMAAFIAKRRNDYEGAFRHY
                     DAMRRQAPNVPNAYVGLADSAMALGRPQEAEAALAKGMRGTSGSVHIHFRRARLAEER
                     QDWPAAEQRWRGMWKEFEDPRAAVAAAECCMRRGDLQAADALLRQAVEAVPEAPDVWR
                     AYARLAGQRGDAAEQARRWEAARLRHPALVPDS"
BASE COUNT          776 a         1559 c         1645 g          797 t
ORIGIN      
        1 agcgcagcca tgtgccgggg accaaggcgg aactggcgat caagggcgag ttcgtgccgc
       61 tgacgcaaag cgctgccgcg aaaacgctgt tcgaactcta cgtgtccgcc gccgcggata
      121 ccggattcag gaccgagggc gagttcaccg gcggctgcgc ggattccgga ttcaccgcgg
      181 cggtcggcgc gccgaccatc tgcgcggtgg ggccggtcgg cggcaaggcg cacagcccgg
      241 acgaattcct gcggctggac agtctggtgc cgcgcgccca ggcctgcgcc cgcgccatcc
      301 tgcggctgga gcgggtggga ctgtagcgcc gcggcgccct ctccgccggg tggggcggag
      361 aggggcggct accgcccgtc gtgcccgtgg tgccagccgc cgtgccagtc gtggtcaccc
      421 cagtcgtgat cgccccagtc atggtctccg ccgaaaccga aggccacata acccgggttg
      481 tagccgtaat acggcgccgg ggcataacca tagccatagc catagttgct gtagccgtag
      541 taatcatccg gataagcgac gcagccgccc agcacggccg ccgcgcccag tgccaccacc
      601 ggaagcagcc agcgaaaccg cctggaacgg acgcggagcg agcgcgtcat ggtctctctc
      661 ccctcaggtt gcggccgaga ggtaggtgag gggcggggcg gtttcatgac cagcgcaagt
      721 gcaaaggatg gcgagacgaa gctcgcgcga tcgttatagc gcccgcatca agcgtgacac
      781 accggacgcg catacagcgt cgtggcgggc gcagggccgt catgacggag tctgccagcc
      841 cgggagtcgg aatcaatgcg ccagccggtc gatcatcggc tgggtgatca ggcagacgcc
      901 gcgctcggtg cggcggaagc gcttggcgtc cagttccgga tcctcgccca ccaccaggcc
      961 gggcgggatc gccacgccgc ggtccacgat gacgttccgc aggcacgcgg agcgggcgat
     1021 gctgacatag ggcatcacca cggcgctctc gatctgcgcg taggagtgca cgtgcacacc
     1081 ggtgaacagc agcgattgcc gcacgctggc acccgagatg atgcagccgc ccgagaccag
     1141 cgagctgatc gcctggccgc ggcggccttc caagtcgtgc acaaacttgg cgggcggcac
     1201 gatctcgcca taggtccaga tcggccagtc gcggtcgaac aggtccagtg acggggtggg
     1261 atgcgtcagg tcgatgttgg cttcccaata ggcatccagc gtgccgacat cgcgccagta
     1321 ggcttccgcc tcggcactgg accgcacgca ggaatcggtg aagcggtgcg ccacggcggt
     1381 gccgtgcttc acgatgtgcg ggatgatgtc gccgccgaaa tcgtgccgcg aattcggatc
     1441 ggtggcgtcg cggcagagct ggtcgatcag gaacttcgtt tcgaacacat agatgcccat
     1501 gctggccagc gcccggtcgg gcttgtccgg catgccgggc ggatccttcg gcttttccag
     1561 aaaggaggtg atgcgcccgc tggcatcgat gtgcatcacg ccgaacgcgg tggcatccat
     1621 gcgcggcacc tcgaagcagc ccaccgtgac atcggcgcgc tgctccacgt gctgctgcag
     1681 catgtgctcg tagtccatct tgtagacgtg gtcgccggcc agcacgacga tgtagtccgg
     1741 gctgtagctt tcgatgatgt cgatgttctg gaacaccgcg tccgcggtgc cggcgtacca
     1801 ctgcgtctcc gacacgcgct ggctggccgg caggatgtcg aaggtctcat tgcgctccgg
     1861 ccggaagaag ttccagccac gctgcagatg acggatcagg ctatgcgcct tgtactgcgt
     1921 ggcgacgccg atgcggcgta tgccggagtt gagcgcgttg gacagcgcga agtcgatgat
     1981 gcgcgacttg ccgccgaaat acaccgcggg cttggcgcgg atgtcggtca gttccatcag
     2041 ccggctgccg cgcccccccg ccagcacgta ggccatggag gaacgggcaa gcggggcact
     2101 gaggctggat gcggcagacg gcatgatgtc atcctgtcag tgttggcagg ctcaacgggg
     2161 tgatctggtc ggcgctgcgg tgcgcccagt cgtgctgcgg caccgcgtcc tgcatgcgcg
     2221 cctgcgcctc ggccagttcg gccagcgcgc cggcatggtc gagtgtcacc acacgcccgt
     2281 cctccaccac cctgtggccg tcaacgaaca cgctgtgcac cgcgcgatcg gccgcggtgt
     2341 agatcagcga acgcagcgga tcgcgcaccg gctgcatgaa cggattggtg agatcgatca
     2401 gcacgaggtc ggcgcgcgcc ccgggtgcca gccggccgag atcgtcgcgg cccaaggcct
     2461 gggcgccgcc gatggtggcg gcgtggaaga tggcttcggt gctggtggcg gtgatgtcgc
     2521 gtgcggcgat gcgggccagg atcgccgcca gccgcatctc ctcgatcagg ttatgcggcg
     2581 acacatccgt gccgatcccg acattcactc ccgcccggcg gtaacggccg aaatcctcca
     2641 gcatctgtcc gtagcgcgcg aagggcgagg gacaatgcgc cacgctggcg ccggtttccg
     2701 cgagcagccg gatgtcgtcc cgcgtgtgcc agcgcgtcca ggagtgttcg tcggtgaaga
     2761 tggcgtggcc gaggatggtg cgcgggccga gaatgccgat gtcgtgtgcc cagcttatcg
     2821 gcgtcttgcc gtgccggtgc agcatctcgt tgaactcggt gacgctctgc gcgcaatgcg
     2881 tggtgagggg caggttgttc tcggccgccg cggcggcgct gtcgcgcagc agggattcgc
     2941 tgcaggtgtc gatctgcccg gggaagacga taccggacag ccggccactg ggatggcgcc
     3001 gcgcgcggtc gatcagcgcc agcgcatcct gcaggccgcg ctgaccggcc ggttcgtccc
     3061 acgcgtagtg cagttgccag ccgttctcca ggcgccagcg cgcagaggca aagccgggcg
     3121 ccaggaacac gcgcagcccg ctggaggcgg cgaggtcgat ccaccacgga tcgggcggag
     3181 agaggtcggc caccgtggtg atgccggtgc gcagcatctc gcaatacgcc accgtcttgc
     3241 cggcgcggcg cccgtcgctg tcggtgcgga aggcgccgaa gcgttcgtac agcccggtca
     3301 tgtacatcgc cggcacgccg tgctcctccc ggatgccgcg gaagaacggc tcggtgctgg
     3361 gatgggcgtg gatgtccacc aggccgggga tcagcaggcg gtcgcggccg tcgatgatgg
     3421 tgtcggcggg gccggcgtag ttcttgccga catggacgat ggcattgccg ctgaaggcca
     3481 ggtcagcgtc acgcaggtag gcatggcgcc cggaggccct gtcccaggcg acaatccagg
     3541 ccgcgttgcg gatccaggtg gtctgagtca ttgcgcctca accatggccg tttcgcattg
     3601 tcgccggaaa cgggcgcctg tccaccggaa ccggtcatgg cgggacgctt catcgcgttg
     3661 ttatgaatcc gggacaagag ccgggtgccg gaggcgggcg gcttcccagc gccgagcctg
     3721 ctcggctgcg tcgcccctct ggccggcaag acgggcatag gcccgccata cgtcgggcgc
     3781 ttccggaacc gcttccacgg cctgccggag caacgcgtcg gcagcttgga ggtcgcctcg
     3841 ccgcatacag cactccgcgg cggcgacggc ggcgcgggga tcttcgaact ccttccacat
     3901 cccccgccag cgctgctcgg ctgctggcca gtcctgtcgt tcctcggcca gtcgcgctcg
     3961 acggaaatga atgtggacgc tgccgctggt gccacgcatg cccttggcga gagccgcttc
     4021 cgcttcctgc gggcgaccga gcgccatcgc gctatccgcc aaaccgacat aggcgttcgg
     4081 aacgttgggc gcttggcggc gcatcgcgtc atagtggcga aacgccccct catagtcatt
     4141 ccgtcgcttt gcgatgaacg ccgccatttc ccaaaggaac acgcgattgg ggaaatcctg
     4201 tagggctgtt cggatcagag aatccgccgt agcagaatcc tcgatctcga tcgccaacag
     4261 cgcagccttg gtcgacgccg ccacgatagc cgggcaggcc gcgaggactg gttgccagag
     4321 ctcgcgggcc ctggccatgt cgccgcggtc gatggccgct cgggcttcgt ccagcacggg
     4381 agtaaatcgt tcgactgcca gcgtgatctc ttccacgtgt cgtttgcggt actgccgcat
     4441 tgtgcgacgg cgaaaccatg cgtcaaggat caattcggcc cccagattgc cttcggcgct
     4501 tctcggattt accaaaccgc agcagatgca catctgctcc gattgtgcca gccacgcctc
     4561 gcgtgcaagg taacggcgcg acaggggcat cttctataag accagttgtg gctttggtag
     4621 caatcctgct tattccacgg ccttcgctcg ctcaaggcgc ttccggtatt ctagcaggaa
     4681 cattcgcgct gttcctgcca ccaccggcgt tcagtatacc gtcgccgtcg ttggaacccg
     4741 ccaccacgac cggtccggaa atgcccgggc cgctgat
//
LOCUS       DASSZH010000395         2861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1117418, whole genome shotgun sequence.
ACCESSION   DASSZH010000395 DASSZH010000000
VERSION     DASSZH010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117418"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..819)
                     /locus_tag="VFN42_08605"
     CDS             complement(127..819)
                     /locus_tag="VFN42_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET8996715.1"
                     /translation="MMHAALQSRQTQTTVVVGRPASPDALDVLEQFGTTVRAEREQEV
                     FAQADAAEYCWRVVSGCVRTVKLMEDGRRAVGEFLLAGDYFGFDDIGTYDLSAEAVSN
                     VVLRRYPRRMVESLAERHSGLAQRLRTMALTNLRTAHDRALSLGRKTAVERIAAFLLE
                     MERRSAPQDGPVLELPMCRTDIADHLGLTVETVCRTLAFLKREGMIAITRTGVQLLSR
                     QGLAEVACEARH"
     gene            957..1580
                     /gene="fixJ"
                     /locus_tag="VFN42_08610"
     CDS             957..1580
                     /gene="fixJ"
                     /locus_tag="VFN42_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019087322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator FixJ"
                     /protein_id="HET8996716.1"
                     /translation="MAEQPQIHVIDDDEAVRSSLALLLESAGWRVRCYPSAAAFLQVA
                     SQLEVGVVLTDVRMPEMDGLALQRRLGEIGSRLPVIIMTGHGDVPIAVEALKAGATDF
                     LEKPFDDTHLLEAIRIAVATSQRVHEQVAATAGIAGRLGTLTPREREVLDRLVAGQPN
                     KTIAYDLGTSPRTVEVQRARVMEKMGAHSLPELVRMVIAVQSATGRP"
     gene            1577..2698
                     /locus_tag="VFN42_08615"
     CDS             1577..2698
                     /locus_tag="VFN42_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET8996717.1"
                     /translation="MTDQDLDSALLTSILDTVPDAMVVINQSGIVQSFSRAAERLFGY
                     DAAEVVGRNVAMLMPSPYREQHDDYLARYLATGERRIIGIGRVVVGRRKNGSTFPMEL
                     SVGEVQQDGNRLFTGFVRDLTERQQTQARLQELQNDLLHVSRLRAMGQMAAALAHELN
                     QPLTATANYTRAAQRLLDSPAPDLDRIRQALALANEQTLRSGEIIRRLRAFVARGEVA
                     RLPVEPQKLIEEASALALVGAQERGITVRMDIPPGLPPVLADRVQMQQVLLNLIRNAM
                     EAMEETHPRLLSIGADRQGPWVALRVADTGPGIAPAIQEQLFQPFVTTKQQGMGIGLS
                     VCRTIVEAHGGRLWAEPNPGGGTVFRFTLPIADAPQPGA"
BASE COUNT          437 a          997 c         1013 g          414 t
ORIGIN      
        1 gccggcttcg ccagcccgga gatcctgcag ctgttcgagc tggtgcacgg cgtgccggcg
       61 ctgctgaccc ggctgcgcga gctgccgacc ggcgcggcgg cgccggccga acggctgtaa
      121 cccggctcag tggcgcgctt cgcaggccac ctcggccagc ccctggcgcg acagcagctg
      181 cacgccggtg cgggtgatgg cgatcatgcc ttcgcgcttg aggaacgcca gggtgcggca
      241 caccgtctcc accgtcaggc cgaggtggtc ggcgatgtcg gtgcggcaca tcggcagctc
      301 cagcaccggg ccatcctgcg gcgcgctgcg gcgctccatc tccagcagga aggcggcgat
      361 ccgctccacc gcggtcttgc ggcccaggct gagcgcgcgg tcatgcgcgg tacgcaggtt
      421 ggtcagcgcc atggtgcgca gccgctgcgc gagaccggag tggcgttcgg ccagcgactc
      481 gaccatgcgg cggggatagc ggcgcagcac cacgttgctg accgcttccg cggagaggtc
      541 gtaggtgccg atatcgtcga aaccgaaata gtcgccggcc agcaggaact cgccgaccgc
      601 acggcggccg tcttccatca gcttcaccgt gcggacgcag ccgctgacca cgcgccagca
      661 atattcggcg gcatcggcct gggcgaagac ctcctgctcg cgttcggcac ggaccgtggt
      721 gccgaactgc tccagaacgt ccagcgcatc cgggctcgcg gggcggccca ccaccacggt
      781 ggtctgggtc tgccggctct gcagggctgc gtgcatcatc ttgtctctcc tcggcgttcg
      841 gtgacgcaga cctagccatc cggagggggg tgccgaaatt cggattggga cgtacgtgct
      901 ggtacgtacc ggaaccattg cctcccacca tcccgccgcc ctaaatcagt atcggaatgg
      961 cagagcagcc ccagatccat gtgatcgacg atgacgaggc ggtgcgcagc agcctggcac
     1021 tgctgctgga atccgccggc tggcgggtgc gctgctaccc ctccgcggcc gccttcctgc
     1081 aagtcgcctc ccagttggag gtcggcgtgg tgctgaccga tgtgcgcatg ccggagatgg
     1141 acgggcttgc cctgcagcgc cgactcgggg aaatcggctc ccgcctgccg gtcatcatca
     1201 tgaccgggca tggcgacgtg cccatcgcgg tggaagcgct gaaggccggc gccaccgatt
     1261 tcctggaaaa gccgttcgac gacacgcacc tgctggaggc catccgcatc gcggtcgcca
     1321 ccagccagcg cgtgcacgag caggtcgccg ccaccgccgg catcgccggc cggctgggca
     1381 ccctaacccc gcgggagcgg gaggtgctgg accggctggt ggccggacag cccaacaaga
     1441 cgatcgccta tgatctgggc acctcgcccc gcaccgttga agtgcagcgt gcgcgggtga
     1501 tggagaagat gggcgcgcac agcctgcccg agctggtgcg catggtgatc gccgtgcagt
     1561 ccgccaccgg caggccatga ccgaccagga cctggattcg gccctgctga cctccatcct
     1621 ggacaccgtc ccggacgcca tggtggtgat caaccagagc ggcatcgtgc agtcgttcag
     1681 ccgcgccgcc gaacggctgt tcggctatga cgcggcggag gtggtcggcc gcaacgtcgc
     1741 catgctgatg cccagcccct atcgcgagca gcacgacgac tacctcgccc gctacctggc
     1801 caccggcgag cggcggatca tcggcattgg ccgggtggtg gtggggcgcc gcaagaacgg
     1861 gtccactttt ccgatggaac tctcggtcgg cgaggtgcag caggacggca accggctgtt
     1921 caccggcttc gtccgcgacc tgaccgagcg ccagcagacc caggctcggc tgcaggagct
     1981 gcagaacgat ctgctgcacg tgtcgcggct gcgcgccatg gggcagatgg ccgccgccct
     2041 ggcgcacgag ctgaaccagc cgctgaccgc caccgccaac tacacccgcg ccgcgcagcg
     2101 cctgctggac tcccctgccc cggacctgga ccggatccgc caggcgctgg ccctggccaa
     2161 cgagcagacg ctgcgcagcg gggagatcat ccgccggctg cgcgcgttcg tcgcccgcgg
     2221 cgaggtcgcc cgtctgccgg tggagccgca gaagctgatc gaggaggcca gtgccctggc
     2281 cctggtcggc gcccaggagc gcggcatcac cgtgcgcatg gacatcccgc ccggcctgcc
     2341 gccggtgctg gccgaccggg tgcagatgca gcaggtgctg ctgaacctga tccgcaatgc
     2401 catggaggcg atggaggaga ctcacccgcg cctgttgagc atcggcgcgg atcgccaggg
     2461 cccctgggtg gcgctgcggg tggcggatac cgggccgggc atcgcgccgg ccatccagga
     2521 gcagctgttc cagccctttg tcaccaccaa gcaacagggg atgggcatcg ggctgtcggt
     2581 ctgccgcacc atcgtggaag cgcatggcgg ccggctatgg gcggagccca atcccggcgg
     2641 cggcaccgtg ttccgcttca ccctgccgat tgccgacgcg ccgcagcccg gcgcttgagc
     2701 acaagcgctc ggccgagcgg cggggggacg gatggtcagg cggggcgggg gatcggcggg
     2761 tcgggctgcg acggagagtc cggcagcacc aagggcgcat ccggatcctg gcggatgagc
     2821 gcggcgccga aattgccgtc gcacagcatg gagtcgatgc c
//
LOCUS       DASSZH010000396         2738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_77446, whole genome shotgun sequence.
ACCESSION   DASSZH010000396 DASSZH010000000
VERSION     DASSZH010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77446"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VFN42_08620"
     CDS             <1..263
                     /locus_tag="VFN42_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996718.1"
                     /translation="SVDGGYTWLNRSVPMYWPKGPAQLTHTMSGFDALIYSQALPPGL
                     DFVPVRCFGPVCVARRPGGCAPIPEMPMPYPKPLAQFRPAAP"
     gene            471..1589
                     /locus_tag="VFN42_08625"
     CDS             471..1589
                     /locus_tag="VFN42_08625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HET8996719.1"
                     /translation="MLLGLGGWLVYAWALRGQIAEDSMVFHIAARAWIDGLGSRLYDG
                     FWITQQINQHFAAWLAKPLPLHPWVYPPSFLLLTLPFGYLPFAVAFPVFELASFAAFA
                     AAARSQVTKGRFMLVALLLSPATAANVLLGQNGFITGALLVGGMGLLRSSPVVAGVLL
                     GLMSYKPQFCMLIPVALLASRSWRTAVVAACTAAVLALASVAVFGIHPWQSWVGLMSG
                     GSGLFHDWVREARINGMSVYACAVLLGASPGVANLAQATAILVSGAAVYRAFAFHGAM
                     REQVRLAVFLAATVLAAPHVANYDAVLLAMSAILVVSVVSQEGFGARECVLASLIWLC
                     PLFNPPAAFRIGLLTPILLLVFIGVLVSQARQPEQCAA"
     gene            1735..2688
                     /gene="argC"
                     /locus_tag="VFN42_08630"
     CDS             1735..2688
                     /gene="argC"
                     /locus_tag="VFN42_08630"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HET8996720.1"
                     /translation="MTKATVFIDGESGTTGLGIRDRLAALPQVELRSLPAAQRRDPAA
                     RRDMMAQVDLVVLCLPDDAARESVSLADGLGKAAPKILDASTAHRVHPDWTYGFPEML
                     PGQADRIGAARKVANPGCYPTGAIGLIRPLVEAGIIPADFPICINAVSGYSGGGKAMI
                     EAHEREGGPAFELYGLKLEHKHLPETQLNAGLTRRPIFVPSVGHYRQGMLVSVPLHLD
                     ELPGKPGAADLAAALQAHYRGCDLVRVTPPPANGKLEPEALNDTDRLELYVHANEAHR
                     QAVLVARLDNLGKGASGVAVQNIGLMLGLHAGVAARERVDA"
BASE COUNT          379 a          888 c          935 g          536 t
ORIGIN      
        1 ggagcgtgga cggcggctac acgtggctga accggtcggt gccgatgtac tggccaaagg
       61 gtccggccca gctgacacac accatgtccg ggtttgatgc gctgatctac agtcaggcgc
      121 tgccgccagg actggacttc gtcccggtgc gctgcttcgg tccagtctgc gtggcgcggc
      181 gtcccggagg ctgtgcgccg atccccgaga tgccgatgcc gtatccgaag ccattggcgc
      241 agttccggcc tgcggcgccg tagcgtcagg ggcgaagcgg cgctgtaaag aaggccgcgc
      301 tatgatttgc catggcatgc aggtcacagc tcggcatggc gcccgccagc gaaactcccg
      361 cccggcttct gtcccggggc ccaaccggcg tctctgacgc ccagccgagc gcgttccgtg
      421 gcagccggcg cctgccggag gtgctcgtcg cctttctgct cgttttgtgc gtgcttttgg
      481 gtctcggcgg atggctggtc tatgcctggg cattgcgcgg ccagatcgcc gaggacagca
      541 tggtcttcca catcgcggcg cgcgcgtgga ttgatggtct gggctcccgg ctgtacgacg
      601 ggttctggat cacccagcag atcaatcagc atttcgcagc gtggctggcg aaaccgcttc
      661 cgctgcatcc ctgggtctat ccgccaagct ttctgctcct gacgctgccc ttcgggtatc
      721 tgcccttcgc cgtcgccttc ccggtgttcg agctggcgag tttcgccgct ttcgccgcag
      781 cagcgaggtc acaggtgacg aagggccggt tcatgctggt tgcgttgctc ctgtcgccgg
      841 cgacggcggc gaatgtcctg ctggggcaga acggctttat caccggcgca ctactggtcg
      901 gcgggatggg actgcttcgg tcatcgccgg tggttgctgg cgtgctgctc gggttgatga
      961 gctacaagcc gcaattctgc atgctgatac cggttgcgct cctggccagc cgctcctggc
     1021 ggacggcggt ggttgcggcc tgcactgcgg ccgtgcttgc cctggccagc gtggcggtgt
     1081 tcggcatcca tccctggcaa tcctgggtcg gcctgatgtc cggcggttcg ggattgttcc
     1141 atgactgggt aagggaagcc cgcatcaacg gcatgagcgt ctatgcctgc gccgtgctgc
     1201 tcggcgcgtc tcccggcgtt gcgaacctgg cgcaggccac cgcaatcctg gtcagcgggg
     1261 ccgccgtcta ccgtgcgttc gcgttccatg gcgccatgcg cgagcaagtg cggctcgccg
     1321 tgttcctggc agcgacggtc ctggcggcgc cgcatgtcgc gaactacgat gccgtcctgc
     1381 tggcgatgtc cgccatactg gttgtgtccg ttgtgtcgca ggaaggcttc ggtgcgcggg
     1441 aatgcgtcct tgccagcctg atctggctat gtcccttgtt caatccgcct gccgccttcc
     1501 gcatcgggct gctgacaccg atccttctgc tggtcttcat cggcgtcctg gtgtcgcagg
     1561 cgcgccagcc ggagcaatgc gcggcctgac tgtcagccaa ggcgatcgct ggcctggaca
     1621 cggcgcccaa actgtaccag atcgacacgc cctggcgcgt gttgcgatgc ggcgagggct
     1681 cttgcttttc ccgcgcgggc tgcggacgat tggcttgcgc gaaaggagct gcacatgacg
     1741 aaggcaacag tcttcatcga cggcgaatcc ggcaccacgg gcctgggcat ccgcgaccgg
     1801 ctggcggcgt tgccgcaggt tgagttgcgc agcctgccgg ctgctcagcg ccgcgatccc
     1861 gcagcccggc gtgacatgat ggcgcaggtc gatctggtgg tgctgtgcct gccggacgat
     1921 gccgcgcggg aatcggtgtc cctggccgat gggctgggca aagccgcgcc gaagatcctg
     1981 gatgccagca cggcgcatcg ggtgcacccg gattggacct acggtttccc cgaaatgcta
     2041 cccggtcagg cagaccgcat cggcgcggcc cgcaaggttg ccaatcccgg ttgctatccg
     2101 accggtgcga ttggtctgat ccggccgttg gtggaagccg ggatcatccc ggccgatttc
     2161 ccgatctgca tcaatgccgt cagcggctat tccggtggcg gcaaggcgat gatcgaggcg
     2221 catgaacgcg agggcggtcc cgccttcgaa ctctacggcc tgaagctgga gcacaagcat
     2281 ctgccggaaa ctcagctcaa tgctgggctg acccgccggc ccatcttcgt tccttcggtc
     2341 gggcattacc gccagggcat gctagtttcg gtgccgctgc acctggacga actgcccggc
     2401 aagcccggcg ccgccgacct ggcagccgcg ctccaggcgc attatcgcgg ctgcgacctg
     2461 gtgcgggtca cgcccccacc ggcgaatggt aagctggagc cggaggcgct gaacgatacg
     2521 gaccggctgg aactgtatgt gcatgccaat gaggcgcatc ggcaggcggt gctggtcgcg
     2581 cggctggaca atctcggcaa gggcgcttcc ggtgtggcgg tgcagaacat tggcctgatg
     2641 ctcggtctgc acgccggcgt ggccgcgcgg gagcgtgtgg atgcctgacc acccggccgg
     2701 cttcggcccg attttctcac tggacggcgt caccccca
//
LOCUS       DASSZH010000397         2343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1305042, whole genome shotgun sequence.
ACCESSION   DASSZH010000397 DASSZH010000000
VERSION     DASSZH010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305042"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="VFN42_08635"
     CDS             complement(<1..968)
                     /locus_tag="VFN42_08635"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8996721.1"
                     /translation="MQSFTRRESLALGVAAVGAATLPIVGANAAEDVPTANVAPPDLK
                     IEKGAQLKVLRPAKFVAPDEVYFRENTKKFTQQTGVDVRVDFVSWEDLRPQTAVAANT
                     GAGPDVIIGFSADPQIYANKLVPMDDLADYLGKKYGGWYQLSELYGKKWGSNNWLSIP
                     MGGSGAATVYRKSWVKAAGFDEIPNDVNDFLKLCAALKKNGHPAGFALGHAVGDANGF
                     ANWLLWTHGAALTDEKGKVSLDSKATIDALKYAKELYPHMIGGTLAWNDAANNKAFEA
                     GQISMTFNGVSIYFVAKKSPEKQMQEIAEDTYHQIPPLGAAKARPAS"
     gene            complement(1283..>2343)
                     /locus_tag="VFN42_08640"
     CDS             complement(1283..>2343)
                     /locus_tag="VFN42_08640"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET8996722.1"
                     /translation="TQLLYHSYASWWLCDSGGIFFCAPALVLWLGAEDAPRIIDHSGS
                     LIDTLVLIVTVCAATLLFAVPGVGRLVWPEAVFLLTVPLAWITLRISLRAAYTLLTLI
                     YIIAAVGTMMGNGPFHGPTIVNPLQSVGLMTVLFAMDSLTLIALTSERREAEARLAEV
                     RGTLERAAQRTEYLRRESLTDPLTAIGNRRFFLVHAGAEWDRARAADLHFALVLLDLD
                     HFKQVNDTGGHAQGDAALQAVARTLAMLTRKQDVLARIGGEEFAIALPDTPIEEALEI
                     AERLRRTLLQIGPPGIGALPHQISASFGVAERTHDDLSLDDLLQRADAAVYAAKAKGR
                     NRVELAAAQVDAGQVLPA"
BASE COUNT          373 a          793 c          758 g          419 t
ORIGIN      
        1 gaggccggac gtgccttcgc ggcgcccagc ggcgggatct ggtggtaggt gtcctcggcg
       61 atctcctgca tctgcttctc gggcgacttc ttggccacga aatagatcga cacgccgttg
      121 aaggtcatgc tgatctggcc ggcctcgaac gccttgttgt tcgccgcgtc gttccacgcc
      181 agcgtgccgc cgatcatgtg cggatacagc tctttggcat atttcagcgc atcgatggtc
      241 gccttggaat cgagcgacac cttgcccttc tcgtcggtga gggcggcacc gtgcgtccaa
      301 agcagccagt tggcgaagcc gttggcgtca cccaccgcgt gccccagggc gaagccggcc
      361 gggtggccat tcttcttcag cgccgcgcac agcttcagga aatcgttcac gtcgttcggg
      421 atctcgtcga acccggcagc cttcacccag gacttgcggt acaccgtggc cgcaccgctg
      481 ccgcccatcg gaatggatag ccagttgttg ctgccccact tcttgccgta caactccgac
      541 aactgatacc agccgccgta tttcttgccc aggtagtcgg caagatcgtc catcggcacc
      601 agcttgttgg cataaatctg cggatcggcg ctgaagccaa tgatgacgtc cgggccggcg
      661 ccggtattcg ccgccaccgc ggtctgcggc cgcaggtctt cccagctcac gaaatcgacc
      721 cgcacgtcca cgccggtctg ctgggtgaac ttcttggtgt tctcgcggaa atacacctcg
      781 tccggtgcga cgaacttggc cggacgcagg actttcagct gcgcgccctt ttcgatcttg
      841 aggtccggcg gcgcgacatt cgcggtgggc acatcctcgg cggcattcgc accaacgatc
      901 ggcagagttg ctgcgccgac cgcggcaacg ccgagggcca gcgactcgcg ccgggtgaaa
      961 ctttgcatgc tgtctgtcct ttccttgcac gtcacatctg gcagcagggt gagcgccgcc
     1021 aatgggcctt gccgctcccg ggcagcgccg catgcatcgc aggatgcgac cccgttccgc
     1081 gcttcgggcc gcatgcacag atcgccgcct gcggcagccc gcagtcggcc tacactcccc
     1141 aaattggtct gacagcgtat tgttggcgcg gatgcgctgt aatccgggag gacggcagac
     1201 tcttaccgcc aatgccccgg caaatccata cccgccggta tcaccgtccc atcagcggaa
     1261 cgggacggct caggcgcgcc attcaggccg gcagcacctg tccggcgtcc acctgcgcgg
     1321 cggcgagttc cacccggttg cgccccttgg cctttgccgc atagacggcc gcatccgccc
     1381 gctgcagcag gtcgtccagg ctgaggtcgt cgtgggtacg ctccgccacg ccgaagctgg
     1441 cgctgatctg gtgcggcagg gcaccgatcc ccggcggtcc gatctgcagc agggtgcgcc
     1501 gcagccgttc ggcgatctcc agcgcctcct cgatcggcgt gtccggcagg gcgatggcga
     1561 attcctcgcc gccgatgcgt gccagcacgt cctgcttgcg ggtcagcatg gccagcgtgc
     1621 gggccacggc ttgcagcgcc gcatcgcctt gcgcatggcc gccggtgtcg ttcacctgct
     1681 tgaagtggtc caggtcgagc agcaccagcg cgaagtgaag gtcggccgcg cgggcgcggt
     1741 cccattcggc gccggcgtga accaggaaga agcggcggtt gccgatcgcg gtcagcggat
     1801 cggtcaatga ttcccggcgc aggtattcgg tgcgctgggc ggcacgttcc agtgtgccgc
     1861 gcacttccgc caggcgcgcc tcggcttcgc gccgttcact ggtcagcgcg atcagcgtca
     1921 gcgagtccat tgcgaacagc accgtcatca gtccgaccga ctgcagcggg ttgacgatcg
     1981 tgggtccgtg gaacgggccg ttgcccatca tcgtgccgac ggctgcgatg atgtagatca
     2041 gggtcagcag cgtgtaggcg gcgcgcagcg aaatgcgcag cgtgatccag gccaggggga
     2101 cggtcagcag gaacaccgct tccggccata ccagccggcc gaccccgggt accgcgaaca
     2161 gcagggtggc agcgcacacc gtgacgatca gcaccagcgt gtcgatcagc gagccggagt
     2221 ggtcgatgat gcgcggcgcg tcctcggccc ccagccacag caccagggcg ggtgcgcaga
     2281 agaagattcc tccgctgtcg cacagccacc aggaggcata gctgtgatac agcagttgcg
     2341 tcg
//
LOCUS       DASSZH010000398         2409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_520769, whole genome shotgun sequence.
ACCESSION   DASSZH010000398 DASSZH010000000
VERSION     DASSZH010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520769"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VFN42_08645"
     CDS             <1..339
                     /locus_tag="VFN42_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996723.1"
                     /translation="GPATFTLTWTSERFAKANPKNLPIFVAALRQADKFIEAHPEDAA
                     KLYAASTHTKIPLPDLVKMITDKQVHWTTQPQQTMKYARFMAKIGMIPAAPSSWKEMF
                     FPEITDRPGS"
     gene            351..1142
                     /locus_tag="VFN42_08650"
     CDS             351..1142
                     /locus_tag="VFN42_08650"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8996724.1"
                     /translation="MMAPLLEARGVTLQYKTRRHLVTATYRVDLAVHEGDRFIILGPS
                     GCGKSTLLKAIGGFMLPVEGSISLSGRPVTAPGPDRMMVFQEFEQLMPWKTVLGNVMF
                     PMRAARAFPRQQIRDRAMAALAKVHLAQFAEVHPHMLSGGMKMRVAIARALAMEPAIL
                     LMDEPFAALDAMTRRHMQQELYELWQSLRFTMLFVTHSIEEAVLLGSRILLLSAHPGQ
                     VRAELNAAHLDGRDASDPEVGRLTRRIHDLLFGAAAPVERETVNV"
     gene            1135..2013
                     /locus_tag="VFN42_08655"
     CDS             1135..2013
                     /locus_tag="VFN42_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8996725.1"
                     /translation="MSDFAPGVLLRPEFEVVPQKIGQITAVEAPLSWRERLSGNVAVR
                     RGLVLIGLALLWQAYATWLDNSLLFPSFIETVRVFITGIFSGELSERILTSLRVLLIG
                     YAAGIGLAAVLTTVAVATRIGDDILSVLTAMFNPLPAIALLPLALIWFGLGTPSLVFV
                     LIHGVLWAVVLNTGTGFRAVPETLRMSGRNYGLSGVRYVALILMPAASASILAGLKIG
                     WAFAWRTLIAAELVFGVSSRSGGLGWYIFQAREELETAQVFAGLLAVIVIGLLVESVI
                     FRAIETRTVRRWGMQR"
     gene            complement(2078..>2409)
                     /locus_tag="VFN42_08660"
     CDS             complement(2078..>2409)
                     /locus_tag="VFN42_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996726.1"
                     /translation="GAFRTLLPSPRIAAAPTLAPVLTLQEPRAWPELESVPAHAMIAA
                     KPAPADAVPLNDNQKAILMAFGAHVAKRPLSLPEKQMAGRQLQTHGDARTWLAALMPH
                     LPKPGAS"
BASE COUNT          363 a          760 c          855 g          431 t
ORIGIN      
        1 ggcccggcca ccttcaccct gacatggacc agcgagcgct tcgccaaggc gaacccgaag
       61 aatctgccga tcttcgtcgc cgcgctgcgc caggcggaca aattcatcga ggcgcacccc
      121 gaagacgcgg ccaagctcta cgccgccagc acgcacacca agatcccgct gccggatctg
      181 gtgaagatga tcacggacaa gcaggtgcac tggaccacgc aaccgcagca gaccatgaaa
      241 tatgcgcggt tcatggcgaa gatcggcatg atcccggcgg caccgtcgag ctggaaggag
      301 atgttctttc cggaaatcac cgacaggccc ggcagctaag gggcacgccg atgatggctc
      361 cgttgctcga ggcgcgcggc gtgacgttgc agtacaagac ccggcgccat ctcgtgacgg
      421 cgacctaccg ggtcgacctg gcggtgcatg agggcgaccg cttcatcatt ctcggcccgt
      481 ccggctgcgg caagagtacg ctgctgaagg cgatcggcgg gttcatgctg ccggtcgaag
      541 gcagcatcag cctgtccggc cgtccggtca ccgcgcctgg tcccgaccgc atgatggtgt
      601 tccaggaatt cgagcagctg atgccctgga agaccgtgct gggcaatgtg atgttcccga
      661 tgcgcgcggc ccgtgcgttt ccccgccagc agatccgcga ccgcgccatg gcggcattgg
      721 cgaaggtgca cctggcgcag ttcgccgagg tgcaccccca catgctgtcg ggcggcatga
      781 aaatgcgggt ggcgatcgcc cgcgcgctgg cgatggaacc ggcgatcctg ctgatggacg
      841 agccctttgc cgcgctggac gcgatgaccc gccggcatat gcagcaggaa ctctacgagc
      901 tgtggcagtc gctgcgcttc accatgctgt tcgtcaccca ctcgatcgag gaagccgtgc
      961 tgctgggatc gcgcatcctg ctgctgtcgg cacatcccgg ccaggtgcgg gcggagctga
     1021 acgcggcgca tctcgatggc cgcgacgcgt ccgacccgga ggtgggtcgg ctgacccggc
     1081 ggatccacga cctgctgttc ggcgcggcgg cgccggtgga acgcgagacg gtgaatgtct
     1141 gacttcgcgc cgggcgtgtt gctgcgcccc gaattcgagg tggtaccgca gaagatcggc
     1201 cagatcacgg ccgtggaggc gccgctgtcc tggcgggagc ggctgtccgg caatgtcgcc
     1261 gtccggcgcg ggctggtgct gatcgggttg gcgctgctct ggcaggccta tgcgacgtgg
     1321 ctggacaatt cgctgctgtt ccccagcttc atcgagaccg tgcgggtctt tatcaccggc
     1381 attttctcgg gcgagctgag cgaacgcatc ctgacctcct tgcgcgtgct gctgatcggc
     1441 tacgcggcgg gaatcgggtt ggccgcggtg ctgaccacgg tggcggtggc gacgcgtatc
     1501 ggcgatgaca tcctgtccgt gctgacggcg atgttcaatc cgctgccggc gatcgcgtta
     1561 ctgccgctgg cgctgatctg gttcggcctg ggcacgccgt cgctggtgtt cgtgctgatc
     1621 catggcgtgc tgtgggcggt ggtgctgaac accggaaccg gcttccgcgc cgtgcccgag
     1681 acgctgcgga tgagcgggcg gaactacggc ctgtcggggg tgcgctacgt ggcgctgatc
     1741 ctgatgccgg cggcttcggc ttcgatcctg gcaggattga aaatcggctg ggccttcgcc
     1801 tggcggacgt tgatcgcagc ggaattggta ttcggcgtct catcccggtc gggcggattg
     1861 gggtggtaca tcttccaggc gcgcgaggag ctggaaacgg cgcaggtgtt cgccggattg
     1921 ctggcggtga tcgtgatcgg cttgctggtg gaatcggtga tcttccgcgc catcgagacg
     1981 cgcaccgtcc ggcgctgggg catgcagcgt taggcacgcg cttgcacgtc gtgcaagctc
     2041 cgggccgcag agcggcagag tctatggccg gtcgggatca cgacgcgcca ggcttgggga
     2101 ggtgcggcat caacgccgcc agccacgtcc tggcatcgcc gtgggtctgc agttgccgtc
     2161 ccgccatctg cttttccggc agcgacagcg gccgcttggc cacatgcgca ccgaacgcca
     2221 tcaggatggc cttctggttg tcgttcagcg gtaccgcatc ggctggcgcc ggtttcgcgg
     2281 cgatcatcgc gtgtgccggg acggactcca actcgggcca ggcgcgcggc tcttgcaggg
     2341 tcagcaccgg cgccagcgtc ggggctgcgg cgatgcgcgg actgggcagg agggtgcgga
     2401 atgccccgc
//
LOCUS       DASSZH010000399         3383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_282255, whole genome shotgun sequence.
ACCESSION   DASSZH010000399 DASSZH010000000
VERSION     DASSZH010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282255"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..843
                     /locus_tag="VFN42_08665"
     CDS             322..843
                     /locus_tag="VFN42_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996727.1"
                     /translation="MAHAASLTITDIMGVSTTISANYYSNAFSVNGTSFPFTATDYTG
                     SVTLPDTSSISLTASSYDAGYGVTSGTLYFVPFAGSDQIVAKLVYSASNNGSIGTMLG
                     TLTDAAAGSLGTVPLGTNASDIGVVGSTLAFDEPYWVQRFSVTAPLPEPASGGMLLAG
                     VLGLGVLAQRRRT"
     gene            complement(856..2031)
                     /locus_tag="VFN42_08670"
     CDS             complement(856..2031)
                     /locus_tag="VFN42_08670"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8996728.1"
                     /translation="MRAFLPLLTLFVIRLAPGIAFQSVAVAAPLLMLALGLNHAQAGL
                     ALGVFTLPGLVVSVPAGMLARRIGDSAVLCGALLLVAAGALIAGTAHAYPLLLAGRAL
                     GGAGGVSILMLVLKMTADRYAGPWLATASAVTITAWPAGLALGLLVFGPLPQALGWRA
                     TLLLAGAPALLGLLLVPLIGRAKGGTAAAAHAAGIAPLPPRRFMLGAIATWAMINAVL
                     PIIIGFLPGYLMSQGRDLDAAAAATSLAVWTPAIGIPLGGLLADRLLGRRAAVMLGLT
                     LTAAMLLLIGSGITMGMALAAFGIAFSLAPGPLTAQLGQAMPLRARAVVFGWYSACSY
                     GAMTVGPWVAGWLRDSTGDPRAPLLFAAAVMLLALAPYALMDRAAAALPAVVAPEAG"
     gene            complement(2232..2927)
                     /gene="phoB"
                     /locus_tag="VFN42_08675"
     CDS             complement(2232..2927)
                     /gene="phoB"
                     /locus_tag="VFN42_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HET8996729.1"
                     /translation="MSKPLVLVVEDEAALATMLRYNLEKQGFRVEEATDGQEALTRIA
                     EVQPDIVLLDWMLPVMSGIEVCRQIRRNPNTRDLPVIFLTARTEDQDAVRGLNTGADD
                     YITKPFSTEALLARIRALLRRANAVPAKGQMTFHDISLDLSVHRVTRNNRRIHLGPTE
                     FRLLEFFMQHPRRVFTREELLDAVWGPDIHVEPRTVDVHIRRLRKAINGPDELDVVRT
                     VRTAGYALDTEPL"
     gene            complement(2997..>3383)
                     /locus_tag="VFN42_08680"
     CDS             complement(2997..>3383)
                     /locus_tag="VFN42_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HET8996730.1"
                     /translation="LVLAQFAMPFSLAALGHMARLVQQNLKMIIDSIGDNDVDKAVEV
                     WRGDQVVDDLYNAIFRELITYMMEDPRNITPCTHLLFIAKNLERIGDHATNIAEVVYY
                     AVKGETLPDTRPKGDTSAYAVVRGKE"
BASE COUNT          558 a         1188 c         1142 g          495 t
ORIGIN      
        1 acttgccgct gagcctgccg cggaaagcga gttgacggca tgcacgcgcg gtaagcggac
       61 tgagcgcgcc caaaggattg tcacacccga caatgcgcgc ggtctgcatc gctcgtccgc
      121 ggggggccgg cggggccagc cccctgcttt tccgaatcgg cgctcatcgt ccatgttggg
      181 taacaacgag aaacgagcgg ttgcggaatg cccaacatca tcagcctgcg acaacagaat
      241 aaacacagct gccgaacagg gcagccctgg cggtggtcta tccgccttcc ggtcctgctc
      301 ggctgcctcg gtgcgcctgc cgtggcacac gccgccagcc tgaccatcac cgacatcatg
      361 ggtgtgtcca ccacgatatc ggcaaattat tactcgaacg cgttctcggt gaacggcacc
      421 tcattcccct tcaccgccac cgattacacc ggcagcgtga cgctgcccga tacgtccagc
      481 atcagcctaa ccgccagttc gtatgacgcc ggatacgggg tgacttccgg cacgctgtat
      541 ttcgtgccct ttgccggctc ggatcagatc gtcgccaagc tggtgtacag cgcgagcaac
      601 aatggcagca tcggcaccat gctcggcacg ctcaccgatg ccgctgccgg atcgctcggc
      661 accgtccccc ttggcaccaa tgccagcgat atcggtgtgg tcggctccac gctggcgttc
      721 gacgagccgt attgggtgca gaggttcagt gtcacggcgc cgttgccgga acctgcctcg
      781 ggcgggatgc tgctggcggg cgtgcttggc ttgggcgtgc tggcgcagcg gcgccgcacc
      841 tgacagcggc ccgcgctatc ccgcctccgg cgccaccacc gccggcagtg ccgcggcggc
      901 gcggtccatc agcgcatagg gcgccagcgc cagcagcatc actgccgccg cgaacagcag
      961 cggcgcgcgc ggatcgcccg tgctgtcgcg cagccagccg gcgacccacg gtcccaccgt
     1021 catcgcaccg taggagcagg cgctgtacca gccgaacacc accgcccgcg cccgcagcgg
     1081 catggcctgt cccagttgcg cggtcagcgg cccgggcgcc agcgagaacg caatcccgaa
     1141 cgcggccagt gccatcccca tggtgatgcc gctgccgatg agcaacagca tcgccgccgt
     1201 cagcgtcagc ccgagcatga ccgcggcgcg gcgtcccagc agccggtcgg ccagcagccc
     1261 gcccagcgga atgccgatcg ccggtgtcca gaccgccagg ctggtcgcag cggcagcggc
     1321 gtccaggtcg cggccctggc tcatcaggta gccgggcagg aagccgatga tgatcggcag
     1381 cacggcgttg atcatcgccc aggtggcgat ggcgcccagc atgaagcggc gcggcggcag
     1441 cggcgcgatc cccgccgcat gcgccgccgc cgcggtgccc cccttcgcgc ggccgatcag
     1501 cggcaccagc agcagcccga gcagcgccgg agcgccggcc agcagcagcg tggcgcgcca
     1561 gccaagcgct tgcggcagcg gcccgaacac cagcagcccc agcgccagcc cggccggcca
     1621 ggcggtgatc gtgaccgccg aggcggtggc cagccacgga ccggcatagc ggtcggcggt
     1681 catcttcagg accagcatca ggatcgacac cccgccggcg ccgccgagcg cccggccggc
     1741 gagcagcagc ggataggcat gcgcggtgcc tgcgatcagg gcgccggcgg ccaccagcag
     1801 caacgcgccg cacagcaccg cggagtcgcc aatccgccgg gccagcatgc ccgcgggcac
     1861 gctgacgacc aggccgggca gcgtgaagac gcccagcgcc agcccggcct gcgcatgatt
     1921 gaggcccagc gccagcatca gcagcggcgc ggccaccgcc accgactgga acgcgatgcc
     1981 aggcgccagg cggatgacaa acagcgtgag gagcggcagg aaagcgcgca tccggaaatg
     2041 ctagccggtg gggcgtgcgg cgagcaaatg gacgagggaa caaaggcggg gacaacaaga
     2101 acctccccct ccccttgcgg gagggggccg gggggagagg gccggcatga gttcccgggg
     2161 caacctcgac cgccccctcc ccccaacccc cgcaagggga gggggagttt tgggctcccg
     2221 gtgttcccgc gctacagcgg ttcggtgtcc agggcgtagc cggcggtgcg cacggtgcgg
     2281 accacatcaa gttcgtcggg cccattgatc gccttgcgca ggcggcggat gtgcacgtcc
     2341 acggtgcgcg gctccacatg gatatccggg ccccagacgg cgtccagcag ttcctcacgg
     2401 gtgaacaccc ggcggggatg ctgcatgaag aactccagca gccggaactc ggtcggtccc
     2461 aggtggatgc gccggttgtt gcgcgtgacc cggtgcaccg agaggtccag gctgatgtcg
     2521 tggaaggtca tctgcccctt tgccggcacc gcattggcgc gccgcagcag ggcgcggatg
     2581 cgcgccagca gcgcctcggt gctgaacggc ttggtgatgt agtcgtcggc accggtgttc
     2641 aggccgcgca ccgcgtcctg atcctcggtg cgcgcggtca ggaagatcac cggcaggtcg
     2701 cgcgtgttcg ggttgcggcg gatctggcgg cacacctcga tgccggacat caccggcagc
     2761 atccagtcca gcagcacgat gtcgggctgc acttcggcga tgcgggtcag cgcttcctgg
     2821 ccgtcggtgg cctcctcaac gcgaaagccc tgcttttcca ggttgtagcg cagcatggtg
     2881 gccagcgccg cctcatcttc caccaccagc accaagggct tggacagggc cggtccggtg
     2941 tcgcggagcg gcgtgcgcag cggtctgctg ccggtgacga tcgccgtgtt cacggattac
     3001 tcctttcctc gcaccaccgc gtaggcggag gtatcgcctt tcggccgcgt atccggcagc
     3061 gtctcaccct tcacggcgta gtacaccacc tcggcaatgt tggtcgcatg gtcgccgatc
     3121 cgctccaggt tcttggcgat gaacagcagg tgcgtgcagg gcgtgatgtt gcgcggatct
     3181 tccatcatgt aggtgatcag ctcgcggaag atcgcgttgt acagatcgtc caccacctgg
     3241 tcgccgcgcc agacctccac cgctttgtcg acgtcgttgt cgccgatcga gtcgatgatc
     3301 atcttcaggt tctgctgcac cagccgcgcc atgtggccga gcgcggcgag gctgaacggc
     3361 atggcgaact gcgccagcac gag
//
LOCUS       DASSZH010000400         4748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_197071, whole genome shotgun sequence.
ACCESSION   DASSZH010000400 DASSZH010000000
VERSION     DASSZH010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197071"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VFN42_08685"
     CDS             complement(<1..809)
                     /locus_tag="VFN42_08685"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET8996731.1"
                     /translation="MGLLIDGVWQQDEGDRAKAAAANGGRFLRGESAFRNWVTPDGRP
                     GPTGHDGFAAAPGRYHLYVSLACPWAHRALIMRVLKGLEQIIPVSMTHWLMGEQGWTF
                     SAGEGVVPDPLFNSRYLHELYSRADAQYTGRASVPVLWDQHTQTIVNNESSEIIRMFN
                     SAFDKVGARPGDYYPADKREEIDAVNTRVYDTLNNGVYKAGFAITQAAYEEAVTPLFD
                     TLDWLEERLGQSRFLCGDTLTEADIRLFTTLIRFDAVYHGHFKCNIRRIVD"
     gene            complement(866..1603)
                     /locus_tag="VFN42_08690"
     CDS             complement(866..1603)
                     /locus_tag="VFN42_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HET8996732.1"
                     /translation="MLRGAFLLVLAWTLASPAARAAVSADMALVLVSDVSRSIDDGEF
                     RLQKDGYLAAFTDPRVVAAIRGGTAGAIAVSYVEFAGGEDVRAVLGWTMLRDAASARQ
                     FAERLNAAPRSFRGRTAISSGIDLGAAMLATSGIAAERRVIDVCGDGTNNSGRPVTEA
                     RDAALKSGITINGLAIINDHPVSWAHAHVQPPGGLAKYYREHVIGGPGAFVLEVRDFH
                     SFGEAMTRKLVTEISGRGPPSRYAAAR"
     gene            complement(1688..2341)
                     /locus_tag="VFN42_08695"
     CDS             complement(1688..2341)
                     /locus_tag="VFN42_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET8996733.1"
                     /translation="MMKHEDIWRALDTLAAEYGLSASGLAKRAGLDATTFNPSKRRMP
                     DGRARWPSTESVAKVLNATGASLESFTALVSGARALPGTSTTRAATRRIPLIGLAQAG
                     GEGFFDDGGYPVGGGWDEVSLPEIGDPNAYALEISGDSMEPVYRDGDMVIVSPSAPIR
                     RGDRVVVRTSRGEVMAKQLARRSARRVELKSLNPAHPDYSFDLNEVAWVHRIIWASQ"
     gene            2564..2965
                     /locus_tag="VFN42_08700"
     CDS             2564..2965
                     /locus_tag="VFN42_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996734.1"
                     /translation="MPPEASQTRQAGLDRPRRGRTVQPFRCAEEAWFWTMAALAARRA
                     GTRFPAEEGRVVRPCEPEDVLKCIDTLYRRRRIDLLHARILRVWGDRQRAPNPANPHD
                     RCDWRLWREAIDRMEWMLRVKGIVYEPRFRN"
     gene            3110..3346
                     /locus_tag="VFN42_08705"
     CDS             3110..3346
                     /locus_tag="VFN42_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996735.1"
                     /translation="MAFSIRNLSVLAYANGFTLWHYRSAADGLDRITGPDYFEAATDM
                     LTGGDIMMLSAADGARVVSVAANPDGSVSAAPLV"
     gene            complement(3414..3635)
                     /locus_tag="VFN42_08710"
     CDS             complement(3414..3635)
                     /locus_tag="VFN42_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996736.1"
                     /translation="MWHEPYLETCCRSALHRLFLSGGTGRPGELKDGPCLERLRGMGL
                     SRRRADGRFEITPDGRERHNSEVLLGVRK"
     gene            3718..4482
                     /locus_tag="VFN42_08715"
     CDS             3718..4482
                     /locus_tag="VFN42_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996737.1"
                     /translation="MSDILGTLKALLDFINGLGRPARRALLQPLATVLLLLALYAGWH
                     IQDEGQVTTGLRVAFLDTRAGRAARDREVEAAILRAELYQAAESDRLINQLLTALLQR
                     SPHAARVRLGVIHNGVSGITGIGLLRYDVTNAVAAAGHVAGDNMQNQPLSEWSELLPK
                     LLSGHCEMTAVATLSSLTSRARLQAMGAVSVLACPVADIHSTLLGSVFIVWDAGDTPP
                     DGAALARLMEYAERIGAQIAAALELRVPKSRLGRRY"
BASE COUNT          730 a         1631 c         1613 g          774 t
ORIGIN      
        1 tagtcgacga tacggcggat gttgcacttg aagtgcccgt gatagaccgc gtcgaagcgg
       61 atcagcgtgg tgaacagccg gatatcggct tcggtcagcg tgtcgccgca caggaagcgc
      121 gactgtccca gccgttcctc cagccagtcc agcgtgtcga acagcggggt caccgcttcc
      181 tcgtacgccg cctgcgtgat ggcgaaaccg gccttgtaca caccgttgtt cagcgtgtcg
      241 tagacccgcg tattgaccgc atcgatctcc tccctcttgt cggccggata gtagtcgccg
      301 ggccgggcgc cgaccttgtc gaaggcgctg ttgaacatgc ggatgatttc ggaggattcg
      361 ttgttgacga tcgtctgggt gtgctggtcc cacagcaccg gcacgctcgc ccgcccggtg
      421 tattgcgcgt ccgcccggct gtacaactcg tgcaggtagc ggctgttgaa cagcgggtcg
      481 ggcaccacgc cctcgccggc cgagaaggtc cagccctgtt cgcccatcag ccagtgcgtc
      541 atggagacgg ggatgatctg ctccagcccc ttcagcacgc gcatgatcag ggcgcggtgc
      601 gcccaggggc aggcgagcga cacatacaga tggtagcgtc cgggcgcggc ggcgaacccg
      661 tcatgcccgg tcggacccgg gcggccatcc ggcgtcaccc agttgcggaa cgcgctctcg
      721 ccgcgcagga accggccgcc attggccgcc gccgccttgg cccggtcgcc ctcgtcctgt
      781 tgccacacgc catcgatcag cagccccatg tcgcggcccc gcccctcacg cccgtttgcc
      841 cgttggccgc ccgagggcgg ccggtctagc gtgccgccgc gtagcgcgac ggcggcccac
      901 gcccggagat ctcggtcacc agcttgcgcg tcatcgcctc gccgaagctg tggaagtcgc
      961 gcacttccag cacaaaggcg cccgggccgc cgatgacgtg ctcccggtaa tacttagcca
     1021 atccgccggg cggctgcacg tgggcatgtg cccatgacac gggatggtca ttgatgatgg
     1081 ccagcccgtt gatggtgatg ccggacttca gcgctgcgtc gcgtgcctcg gttaccggcc
     1141 ggccggaatt gttggtgccg tcgccgcaca cgtcgatcac ccggcgctcc gcggcgatcc
     1201 cgctggtcgc cagcatggcg gcaccgaggt cgatgccgct gctgatcgcg gtgcgtcccc
     1261 ggaaggaccg cggcgccgcg ttcagccgct cggcgaattg ccgggcggat gccgcgtcgc
     1321 gcagcatggt ccagccgagc accgcgcgca cgtcctcgcc gccggcgaac tcgacatagg
     1381 aaacggcaat cgcgccggcc gtgccgccgc ggatcgccgc gaccaccctg ggatcggtga
     1441 aggccgccag gtagccgtcc ttctgcagcc ggaactcgcc gtcatcgatg ctgcgcgaca
     1501 catcggagac caggaccagc gccatgtcgg ccgatacggc cgcgcgcgca gccgggctgg
     1561 ccagagtcca tgccaggact agcaggaacg cgccacgcaa catcgccgcc tccccggttc
     1621 cgcgatttgc caaacggcat aagaccgcag accggggggc gcgtccaaga gaattcccgc
     1681 gaggcggcta ttggctggcc cagatgatcc ggtgcaccca ggcgacctcg ttcaggtcga
     1741 agctgtagtc cggatgggcg ggattcaggc ttttcagctc cacccggcgg gcggagcggc
     1801 gggcgagctg cttggccatc acctccccac ggctggtgcg caccaccacg cggtcgccac
     1861 gccggatcgg cgccgaggga gacacgatga ccatgtcgcc gtcgcgatac accggttcca
     1921 tggaatcgcc gctgatctcc agggcatagg cattggggtc gccgatctcc ggcaggctga
     1981 cctcatccca gccgccgccg accggatagc cgccatcgtc gaagaagcct tccccgccgg
     2041 cctgcgccag gccgatcagc gggatccgcc ttgtggcggc gcgcgtggtg gaggtgccgg
     2101 gcagcgcccg tgccccggac accagcgcag tgaaggattc caggctggcc ccggtggcgt
     2161 tgagcacctt ggcgacgctt tcggtgctgg gccagcgcgc ccgcccgtcg ggcatgcgcc
     2221 gtttggacgg gttgaacgtg gtcgcatcca gcccggcccg cttggcgagg cccgaggcgg
     2281 acaggccgta ttccgcggcg agcgtgtcga gcgcccgcca gatatcttcg tgcttcatca
     2341 tcaccctcgc tgcggcgcac gccttatggc gcggccgagg acgcatgcct tggttgaaaa
     2401 ccgggacggc ggccccgggg aatcgagctt gcggagtctt ataggactac gcgccgaatt
     2461 ttctaggaaa aagcacgcag cgggccttgc ctttttctgc aacctatggt tttatgttcc
     2521 cgctttgttc gagctatccg tgttctcaaa aggagaaacc ccgatgcccc cagaagccag
     2581 ccaaacccgg caggccggcc tggaccggcc ccggcgcggc cggacggtgc agcccttccg
     2641 ctgcgccgag gaggcgtggt tctggaccat ggccgcgctg gccgcccgcc gcgcgggtac
     2701 gcgcttcccc gccgaggagg gccgcgtggt gcggccgtgc gaaccggaag acgtgctgaa
     2761 atgcatcgac acgctgtacc gtcgccggcg tatcgatctg ctgcacgcgc gcatcctgcg
     2821 cgtctggggc gaccggcaga gggcgcccaa tccggccaat ccgcatgatc gctgcgactg
     2881 gcggttgtgg cgcgaggcaa tcgaccggat ggaatggatg ctgcgggtga agggaatcgt
     2941 ctacgagccc cggttccgga attaagtcct tgacctaaac cctgtttcgt cgtatacttc
     3001 gtcccagcaa cgggcgcatc ggcgcccggt gacctcccta caacccagga aaattccgcg
     3061 ccgctccatt ccggggcgcg cttgctgctg cccgcaaagg atcaccctca tggccttctc
     3121 cattcgcaat ctctccgtgc tggcctacgc gaacgggttc accctgtggc actaccgctc
     3181 cgccgccgac gggttggacc gcatcaccgg cccggactac ttcgaggccg cgaccgacat
     3241 gttgacaggc ggcgacatca tgatgctgtc cgcggccgac ggcgcccgcg tcgtctcggt
     3301 tgcggcgaac ccggatggca gcgtcagcgc cgcgccgctg gtttaggggc gggagcgtga
     3361 gcccgcggcg ttctcccctc tccgctgcgg aggggaggac gcttgtccgc ccgctacttg
     3421 cgcacgccga gcagcacttc gctgttgtgc cgttcacgtc cgtccggggt gatttcgaac
     3481 cggccgtccg ctcgccggcg cgacagtccc atgccccgca gccgctccag gcagggaccg
     3541 tccttcagtt cgcccggccg gccggtcccg ccggacagga acagccggtg cagcgccgaa
     3601 cggcagcagg tttccaggta aggctcgtgc cacatcgcgc gcatcgttgg ccggcgctgc
     3661 cggcaacgca agtcgcgcgg ccgccgagcg gcgaagtttc aacagaagat tcatgtgatg
     3721 tcggacattc tgggcacgct caaggccctg ctggatttca tcaacggtct cggccgcccg
     3781 gcgcggcgcg cgctgctgca gccgctggcc accgtcctgc tgctgctggc actgtatgcc
     3841 gggtggcaca tccaggatga gggccaggtc acaaccgggc tgcgcgtcgc cttcctggac
     3901 acccgcgccg ggcgcgcggc gcgtgaccgg gaagtggaag ccgcaatcct gcgggcggaa
     3961 ctgtatcagg cggcggaatc ggaccggctg atcaatcagc tgctgaccgc gctgctgcaa
     4021 cgctccccgc atgccgcgcg ggtgcggctg ggcgtgatcc acaacggagt cagcggcatc
     4081 accgggatcg ggctgctgcg ctacgatgtg accaacgcgg tggcggcggc cggtcacgtc
     4141 gccggcgaca acatgcagaa ccagccgctg tcggaatgga gcgaactgct gccgaagctg
     4201 ctatccggcc attgcgaaat gaccgcggtc gccacgctgt ccagcctgac gagcagggcg
     4261 cggctgcagg ccatgggcgc cgtctcggtg ctggcctgcc cggtggccga catccacagc
     4321 acgctgctcg gcagtgtgtt cattgtgtgg gatgccggcg acacgccgcc ggatggagcg
     4381 gcgctggcca gattgatgga gtatgcggag cgcatcggcg cgcagattgc cgcggcgctg
     4441 gagttgcggg tgccgaagtc gcggctgggg cggcggtact agggaagcgg cacaggcgta
     4501 gccgatcgtc cccgggcgga tggctggctc aaggccggcc atgacgtcat gccaggttgg
     4561 atggcgggct ttcgcccgcc accgcgcaca gcaaggttgt tgttgcacgt cggtgatcgc
     4621 cggcgatcca agactttgct gctggcgacg agaaaggaca cgggcggccg ggccaagccc
     4681 ggccatgaca ggggatggcg ttggctgccc ccttactccg ccgccacctt cggcaggaat
     4741 gccgggac
//
LOCUS       DASSZH010000401         2988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1236958, whole genome shotgun sequence.
ACCESSION   DASSZH010000401 DASSZH010000000
VERSION     DASSZH010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236958"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VFN42_08720"
     CDS             <1..573
                     /locus_tag="VFN42_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlrC C-terminal domain-containing protein"
                     /protein_id="HET8996738.1"
                     /translation="VVLADTQDNPGGGGHGDTTELLAELVRQRATGALVCLINDDESA
                     AACHRAGEGAAVSLSLGGKSDGLPFETTARVLKLTDGSFTLTGPMGAGNPANLGPSAL
                     IDIDGVRVIVTSRKMQALDQAIIRHFGVDPAACPILALKSSVHFRADFAPIAERIIVA
                     IAPGPVVADPAILNFRHVRANVRRRPLPAH"
     gene            complement(574..1101)
                     /locus_tag="VFN42_08725"
     CDS             complement(574..1101)
                     /locus_tag="VFN42_08725"
                     /inference="COORDINATES: protein motif:HMM:NF025729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HET8996739.1"
                     /translation="MLHPARDVVVRYQVTGAHGPGDSAQVTIHYASQGRRMRIEPAGR
                     PSYMIVDGVADRMEMVMPSQHVYVEMPYDPQMLMNFKDKDATFTPQGTKTIAGLGCTV
                     YKVDSREHPGQICVTSDGVILRARGQDASHHGSLQAVSVGYSPQPASLFAPPPGFHKL
                     EPAHPPQAGGDGQKH"
     gene            complement(1207..1866)
                     /gene="aat"
                     /locus_tag="VFN42_08730"
     CDS             complement(1207..1866)
                     /gene="aat"
                     /locus_tag="VFN42_08730"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HET8996740.1"
                     /translation="MSRRVVEITPDLMLRAYRHGLFPMAETRRGDRLYWLDPERRGVL
                     PLDRFHLPRRLLRTVLSGPYQVTADRDFSATINGCATPMPGREDTWINPQIERLFGEL
                     HRLGYAHSVETWLDGELVGGLYGVALGGAFFGESMFSFARDASKVALVHLIARLRLGG
                     FRLLDTQFVTMHLAQFGAEEIPREAYKVMLGGAADSPANWLADPDPAALLAEIRALGT
                     R"
     gene            complement(1915..2556)
                     /locus_tag="VFN42_08735"
     CDS             complement(1915..2556)
                     /locus_tag="VFN42_08735"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HET8996741.1"
                     /translation="MPSSPLLQAAHSSTAGSIRQVRRAELPADTTSLARWLIGKLVVR
                     DLPEGRISGRIVETEAYLPGDAACHAFRGITPRNRSLFLPTGHAYVYRAYGTSWMLNV
                     SGGPQGVGAGVLIRALQPLEGMDLMRRARGTVPERDLARGPGRLAAALAVDRGLDGTD
                     LTEPGPLWLGADDTPHGDIGVSVRIGITKDAQRPLRFYLRVCRFVSGPARLNS"
     gene            2555..2938
                     /locus_tag="VFN42_08740"
     CDS             2555..2938
                     /locus_tag="VFN42_08740"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8996742.1"
                     /translation="MTTFTWEHIHLRSPDPAATAAWYEQMLGAEVIRTPQPDGSTRID
                     LDLSGQKVFIAQADPAKTASSPQSPYMGLDHFGLTVTDMDAAVAELKAKDVAFTMEPK
                     TIRPGVRIAFLTAPQGVSIELIQRG"
BASE COUNT          502 a         1007 c         1003 g          476 t
ORIGIN      
        1 gtggtgctgg cggacacgca ggacaatccc ggcggtggcg gccatggcga caccaccgag
       61 ttgctggcag agctggtgcg gcagcgcgcg accggcgcgc tggtgtgcct gatcaacgac
      121 gacgagagtg ccgctgcctg ccaccgtgcc ggcgaaggtg ccgccgtatc attgtcgctc
      181 ggcggcaaat ccgatggact gccgttcgaa accaccgcgc gcgtgctgaa gttgacggac
      241 ggaagtttta ccctgacggg accgatgggt gcaggcaatc cggcgaatct cggcccatcc
      301 gcactgatcg acatcgatgg cgtgcgcgtg atcgtcacca gccgcaagat gcaggcgctg
      361 gaccaggcga tcatccgcca cttcggggtg gacccggcgg cgtgtccgat cctggcgctg
      421 aaatcatccg tgcatttccg tgccgacttc gcaccgattg cggagcggat catcgtggcg
      481 atcgcgcccg ggccggtggt ggctgatccc gcgatcctga atttccgcca tgtccgcgcc
      541 aatgtccggc gcaggccatt gccggcgcac tgatcagtgc ttctgtccat cgccacctgc
      601 ttgtggcggg tgggcgggtt ccagcttgtg gaatcccggc ggcggcgcga acaacgatgc
      661 cggttgcggg ctgtacccga cactgacggc ttgcagactg ccgtggtggc tcgcatcctg
      721 tcctctggcg cgtagaatga cgccgtcgga ggtcacacag atctggccgg gatgctcgcg
      781 actgtccacc ttgtagacgg tgcagcccag gccggcgatc gtcttcgtgc cctgcggggt
      841 gaacgtcgcg tctttgtcct tgaagttcat cagcatctgc ggatcgtacg gcatctccac
      901 gtacacatgc tggctcggca tcaccatctc catgcgatcg gccacgccgt ccacgatcat
      961 gtagctggga cgccccgccg gctcgatccg catgcgccgg ccctgcgacg cataatggat
     1021 cgtcacctgc gcggaatcgc cggggccatg cgcaccggtt acctgatacc gcaccacgac
     1081 gtcgcgcgcc gggtgcagca tcggccgagc ctgggcagcg aatgaaagca tggccgcgag
     1141 cgtagcgatg ccagccggtt tcagcattgt agcctccttg ctgggttggt ccgatgcagt
     1201 ctcaggtcag cgcgtgccaa gcgcgcggat ttccgccaac agtgccgccg ggtccgggtc
     1261 ggccaaccaa tttgccggtg aatcggccgc gccgcccagc atcactttgt aggcctcgcg
     1321 cgggatctcc tcagcgccga attgcgccag atgcatggtc acgaattgcg tatcgagcag
     1381 ccggaatccg cccagccgca agcgcgcgat cagatgcacc agcgcaacct tggaggcatc
     1441 gcgcgcaaag ctgaacatgc tttcgccgaa aaacgcaccg cccagcgcca cgccgtacaa
     1501 gcccccaacc agctcgccat ccagccaggt ttccaccgaa tgggcatagc ctagacggtg
     1561 cagctcgccg aacagccgtt cgatctgcgg attgatccag gtgtcttcgc ggcccggcat
     1621 cggcgtggca cagccgttga tggtcgcgga gaagtcgcgg tccgccgtca cctggtacgg
     1681 cccggacagc acggtccgca gcaggcggcg cggcagatgg aaccggtcca gcggcagcac
     1741 cccccggcgc tccggatcca gccagtacag ccggtcgcca cgccgcgtct ccgccatcgg
     1801 gaacagcccg tggcgatacg cccgcagcat cagatccggc gtgatctcca ccacacgtcg
     1861 cgacatgcgg ctgattgtgg ggccggggcg ggccggatgg aagcaaatcg tgcgttacga
     1921 attgagtcgc gccggtccgc tcacgaagcg gcacacgcga aggtagaagc ggagcgggcg
     1981 ctgtgcatcc ttggtgatgc cgatccggac gctgacgccg atgtcgccgt gcggcgtatc
     2041 atccgcaccg agccagagcg ggccgggttc cgtcaggtcg gtgccgtcca ggccccggtc
     2101 caccgccagc gccgccgcca gccggccggg gccgcgcgcc aggtcgcgct ccggcacagt
     2161 cccgcgggcg cggcgcatca ggtccatgcc ttccagcggc tgcaacgcgc ggatcagcac
     2221 gccggcgcca acgccctggg gaccgccgga aacgttgagc atccaggacg ttccataggc
     2281 gcgatacacg tacgcatggc cggttggcag gaacagcgag cggttacgcg gcgtgatgcc
     2341 gcggaacgcg tggcaggcgg cgtcgccggg caggtaggcc tcggtctcaa cgatgcggcc
     2401 gctgatgcgg ccctccggca ggtcgcgcac gaccagcttg ccgatcagcc agcgtgccag
     2461 gctggtggtg tcggcgggca gctccgcgcg gcggacttgc cggatgctgc cggcagtgga
     2521 tgaatgggca gcctgcagca agggggagga cggcatgacg acgtttacct gggaacacat
     2581 tcacctgcgc tcgcccgacc cggcggcgac cgccgcctgg tacgagcaga tgctcggcgc
     2641 agaggtcatt cgcacgccgc agcccgacgg gtccacgcgg atcgacctgg atctgagcgg
     2701 ccagaaggta ttcatcgcgc aggccgatcc ggcgaagacc gctagctcgc cgcagtcgcc
     2761 ctatatggga ctggaccatt tcggcctgac ggtgaccgac atggatgccg ccgtggcgga
     2821 gctgaaggcg aaggatgtcg ccttcaccat ggagccgaag acaatccggc ccggcgtgcg
     2881 catcgccttc ctgaccgccc cgcagggtgt gtcgatcgaa ttgatccagc gcggctgaaa
     2941 ccctcccgcg cgagccagcg gaatgatcac cctcggccga gcggttcg
//
LOCUS       DASSZH010000402         4446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_861833, whole genome shotgun sequence.
ACCESSION   DASSZH010000402 DASSZH010000000
VERSION     DASSZH010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861833"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="VFN42_08745"
     CDS             complement(<1..1100)
                     /locus_tag="VFN42_08745"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET8996743.1"
                     /translation="MEVEQPPGDESGRRSGPLAGIRVLDLSTVLAAPMAASLLGELGA
                     DVIKVEQPGAGDPLRSFRPLREGQSLHWRVTNRNKRSISLDVSQSKGRDILFGLVRQC
                     DVVVVNFRPPTLRRWGIDYADLKAVRPDIIMLHLSAFGRTGPYADRPGFARIAEAFAG
                     LTHVTGYPDRPPVFAGYPIADGIAGIQGAFSLMVALRHRDLTGEGQLIDLALYEPLLR
                     LMEDFIVGHGSDGTVKERVGSAQPNIAPNNLYPTADESFVVIPASTEAIWRRLASLVD
                     RGMIERFPSNEARVAHREEIDAAICGFTRRHTADALVEICEDVGVPCGKLNSVRDIFS
                     DPHIRARGNLQRVFDPSLDAWLTMQAPIPSFS"
     gene            1200..2159
                     /locus_tag="VFN42_08750"
     CDS             1200..2159
                     /locus_tag="VFN42_08750"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET8996744.1"
                     /translation="MANITIRQLKAFIFVAQERSFARAAERLHISPSAVTLAVRDLES
                     EVGLRLFDRSTRSVELTPQAVTFLPVDERILGDLEGALEDLRSLAERQKGSVVVAAAA
                     SFINIVLTPAVAQLARTHPGIRVRIIEDTTESLVRRVVSGEVDFGVTTLWRPVTGIDA
                     VQMLCDRLGVMFPSGHPMALSEAPIEWSEVVGLPMASLAADAGIRAQIENHPEIGRLL
                     PRPLYEVSNVSALQALVQHGAGVAVVPWMSARRASGGSMNYRPLTAPCVWRELFLIRR
                     ERRSLGPAAAEMAKFMLDKLRALAPSEYVRANPCLTADTLAAP"
     gene            complement(2400..3761)
                     /locus_tag="VFN42_08755"
     CDS             complement(2400..3761)
                     /locus_tag="VFN42_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET8996745.1"
                     /translation="MNDVALGDTLLATDQRHLIHPLHHPKEHTNAQIFVEGRGAMLRT
                     ADGREYIDGLSALWNVNVGHGRKELADAAQAQMAKLAYASAYAGFSNEPAIRLAEKLV
                     SIAYDNIAGVYFTTGGAESNESAFKFARYYWKRMGKPDKVKIISRIYGYHGVTMAAMS
                     ATALPGYGKMFGPLVPNFHQVAPPYPYRWPGNGDAGIGAADAVEEMIQAQGADNVAAV
                     IAEPVMGAGGVIVPPPTYFPRLREICDRHNVLLIADEVITGFGRTGRWFALGHWGVQP
                     DLVSFAKGVTSGYLPLGGVMVSKRIHQAIEDAPMSERFMHAATYSGHPACCAVGLANV
                     ALIEREGLVERAAVMGKRLLSGLETLRGLPVVGDVRGLGMLAAVELVEDQGSRKPAAG
                     LGAKVVAEAMKRGLIIRQRGASDGPPASGDSLCLAPPLMTPEATLDRIVSIVGESIEA
                     ACR"
     gene            4047..4379
                     /locus_tag="VFN42_08760"
     CDS             4047..4379
                     /locus_tag="VFN42_08760"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HET8996746.1"
                     /translation="MTPTQFAALWKAVQLGKVTQNLLGRLTAMDPATIQGVVRRLIER
                     GLLDRSEDPMDRRTAVLAPTEAGIALVCQGVACAQRSHGAVLAPLTEAERRQLLSLLR
                     KIGDPNGQ"
BASE COUNT          726 a         1507 c         1481 g          732 t
ORIGIN      
        1 ctggagaagc tggggatcgg ggcttgcatc gtgagccacg catccagtga gggatcgaag
       61 acgcgttgca gattgcctcg cgcgcggatg tgcgggtcgg agaaaatgtc gcgaacggaa
      121 ttgagcttgc cgcagggcac gccgacatcc tcgcagatct ccaccagcgc gtcggccgtg
      181 tgacggcggg tgaacccgca gatcgcggca tcgatctcct cgcgatgcgc gacgcgcgcc
      241 tcgttcgacg ggaagcgctc gatcatcccg cgatccacca gagacgcgag cctgcgccag
      301 atcgcctcgg tggaggcggg gatcacgaca aatgattcat ccgctgtcgg atacaggttg
      361 ttcggcgcga tgttgggctg cgcgctgccg acccgctcct tcactgtgcc atcggagccg
      421 tggccgacga tgaagtcctc catcagccga agcagcggtt cgtacagcgc aaggtcaatg
      481 agctggccct cgccagtcag gtcgcggtgg cgcagcgcaa ccatcaagga aaacgcaccc
      541 tggatgccgg cgatgccgtc cgcgatcgga taaccggcga acaccggcgg gcgatccgga
      601 tagccggtga catgggtgag gccggcgaag gcttccgcga tgcgggcgaa acccggccgg
      661 tcggcatagg gcccggtgcg cccgaacgca gacaaatgca gcatgatgat atccggccgg
      721 acagccttca gatccgcata gtcgatcccc catctgcgaa gcgtcggcgg ccggaagttc
      781 accaccacca catcgcactg gcggaccaga ccgaacagga tatcgcgccc cttcgattgg
      841 ctcacgtcga gcgagatgct gcgcttgttg cggttcgtca cccgccagtg gagggactgc
      901 ccttcacgca gcggacgaaa cgaccgcaac gggtccccgg ccccgggttg ctcgaccttg
      961 atgacatccg caccaagctc gcccagcagg cttgccgcca tcggggctgc gagcaccgtg
     1021 gacaggtcga gcacacgaat gcccgcaagc ggtccactcc ggcgaccgga ctcatcgccc
     1081 ggtggctgct caacctccat ccttggatcc atccccgtcc ttcttgatgg aaacgctatg
     1141 gacgaagcgg aaaagcgtca attataataa actggcagat tatcgagaat acctcaataa
     1201 tggccaacat caccatccgc cagctgaaag cgttcatttt cgtcgcgcag gagcgcagct
     1261 tcgcgcgcgc cgcggaacgc cttcatatct ccccctcggc ggtgacactg gcggtgcggg
     1321 acctggaatc cgaggtcggt ctacgcctgt tcgaccgttc cacgcggtcg gtcgagctga
     1381 caccgcaggc ggtcacgttc ctgccggtgg atgagcgcat cctcggcgac ctggagggtg
     1441 cgctggagga tctgcgttcc ctggcggagc gccagaaggg ctccgtcgtg gtcgccgcgg
     1501 cggcgtcgtt catcaacatt gtccttacgc ctgcggtcgc gcagttggca cgaacgcatc
     1561 ccggcatccg tgtgcggatc atcgaggaca ccaccgagag cctggtgcgg cgggtggtga
     1621 gcggggaagt cgatttcggc gtcacgacac tgtggcggcc ggtcaccggg atcgacgcgg
     1681 tccagatgct gtgcgatcgc ctcggggtga tgtttccatc cggccatccg atggcgctgt
     1741 cggaggcacc gatcgaatgg tccgaggtcg tcggcctgcc catggcctcg ctggccgcgg
     1801 acgctggcat tcgcgcgcaa atcgagaacc accccgagat cggtcggctg ctgccgaggc
     1861 cgctctacga ggtttccaac gtctccgcgc tgcaggcctt ggtgcaacac ggcgctggtg
     1921 tcgcggtggt gccgtggatg tcggcgcggc gcgccagcgg gggatcgatg aattaccgcc
     1981 cgctcaccgc tccctgcgtg tggcgcgaac tgttcctgat acgccgggag cggcgcagtc
     2041 tgggaccggc ggccgccgaa atggcgaagt tcatgctgga caaactacgc gcgctcgcgc
     2101 cgagcgagta cgtcagggcc aatccctgcc tgaccgcgga tactcttgcc gccccttgat
     2161 cccgccgcag acggtccgcg agaccgtgcg agcaaagccg ctgcttgaag cgcttcggtg
     2221 attgggatgg cgcaccgggg ccgctgacga atacggctcg ggcgtttcca tccgtgccag
     2281 aggcggctga tggaagctac accgtctcgg cgcggtcatt ggcggggggc gtactccgcc
     2341 gccggacgcc atcagcgttc atccgcgttc gctgatgggt ccagcccgcg tgccggccgc
     2401 taccggcagg ccgcctcgat cgactcgccg acgatcgaca cgatgcggtc cagcgtcgcc
     2461 tccggcgtca tcagcggcgg cgccaggcag agcgaatcgc cgctggccgg cggaccgtcg
     2521 ctggcgccac gctggcggat gatcagcccg cgcttcatcg cctcggccac cactttggcg
     2581 cccagaccgg cggccggctt gcggctgccc tggtcttcca ccagttccac cgcggccagc
     2641 atgcccaggc cgcgcacgtc acccaccacc ggcaggccgc gcagcgtctc cagcccggac
     2701 agcagccgct tgcccatcac cgcggcgcgc tccaccagac cctcgcgctc gatcagcgcg
     2761 acgttggcca ggccgacggc gcagcaggcc gggtggccgg aataggttgc ggcgtgcatg
     2821 aagcgctcgc tcatcggcgc gtcctcgatc gcctggtgga tgcgcttgga caccatcacc
     2881 ccgcccagcg gcaggtagcc ggaggtcacc cccttggcga aggacaccag gtccggctgc
     2941 acgccccaat ggccgagggc gaaccagcgg ccggtgcggc cgaagccggt gatcacctcg
     3001 tcggcgatca gcagcacgtt gtgtcggtcg cagatctccc gcagccgggg gaaataggtg
     3061 gggggcggca cgatcacgcc cccggcgccc atcaccggtt cggcgatcac cgccgcgacg
     3121 ttgtcggcgc cctgcgcctg gatcatctcc tccaccgcgt cggccgcgcc gatgccggca
     3181 tcgccgttgc ccggccagcg atacgggtag ggcggcgcca cctggtggaa gttcggcacc
     3241 agcggcccga acatcttgcc gtagccgggc agggcggtgg cgctcatcgc cgccatggtc
     3301 acgccgtgat agccgtagat ccgcgagatg atcttcacct tgtccggctt gcccatccgc
     3361 ttccagtagt agcgggcgaa cttgaaggcg ctctcgttgg actcggcgcc gccggtggtg
     3421 aaatacacac ccgcgatgtt gtcgtaggcg atgctgacca gcttctccgc cagccggatc
     3481 gccggctcgt tggagaaccc ggcataggcg gacgcatagg ccagcttcgc catctgcgcc
     3541 tgcgccgcat ccgccagttc cttgcggccg tgcccgacat tgacgttcca cagcgccgac
     3601 agcccgtcga tatattcccg cccgtccgcg gtgcgcagca tggcgccgcg gccctccacg
     3661 aagatctggg cgttggtgtg ctccttgggg tggtgcagcg ggtggatcag gtggcgctgg
     3721 tcggtggcca gcagcgtgtc gccgagtgct acgtcattca tcgcgtttcg ccctgggttg
     3781 aatgggcgga acggtgcgcc attccgcgcc cgggtcaaga gccaaggcat cggggcagat
     3841 cggctccccg cgacttgaaa cttgacttcc gcggtagtcg tttgtaagcg aacgatagac
     3901 tgcctgggga ggcaaagctg gcatcgcacg atggcccgcc gcctcagtgc ggcgacttcc
     3961 tgctcgaaga tcacgcgggt ttcctgctgc gccgagcgca tcagagacat gcggcgctgt
     4021 tcctggaggc gagctgcggc tgcgggctga ctcccaccca gttcgcggcg ctgtggaaag
     4081 ccgtgcagct gggcaaggtc acccagaacc tgctcggccg cctcaccgcg atggaccccg
     4141 ccaccatcca gggcgtggtt cgccggctga tcgagcgcgg cctgctggac cggtcggagg
     4201 acccgatgga ccgccgcacc gcagtgctgg cacccacgga agcggggatt gccttggtat
     4261 gccagggcgt ggcatgcgcg caacgcagcc acggggccgt gctcgcgcca ttgaccgagg
     4321 cggagcgccg ccaacttctg tcgctgttgc gcaagattgg cgacccgaac ggacaataag
     4381 aatggatgcc gtgatacctc ccgagccgcc cgccctgggc ttcggcttca gcttcgccga
     4441 cctcgc
//
LOCUS       DASSZH010000403         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_930002, whole genome shotgun sequence.
ACCESSION   DASSZH010000403 DASSZH010000000
VERSION     DASSZH010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930002"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..749
                     /locus_tag="VFN42_08765"
     CDS             258..749
                     /locus_tag="VFN42_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996747.1"
                     /translation="MHFTSQKLIRRLLPVMSGAGCMIALLLAMNVIADWKNQAPRIGD
                     LIAFEPARDALLPESVRIAVETSSGGHCVLDAYTLRRSGGSFMVSRGLAGAEARYIVH
                     WAGARTAEGTASCGDSADLILTRRQLDRLGEAAAAGGIRQAAAAKVVSSPRGPTSRPE
                     SPG"
     gene            complement(703..1371)
                     /locus_tag="VFN42_08770"
     CDS             complement(703..1371)
                     /locus_tag="VFN42_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HET8996748.1"
                     /translation="MMAGSHVALGAAAWLAASPHLGLGPADPAGIALAAAASLLPDID
                     HPKSWVGSRLRPVSMLVSAIFGHRGITHSALAVAACAWVLLQGGAPRWATDAVAVGYA
                     SHLAADMLTPNGLRLAWPVRRTWSLPLCKSGSQAEPLVVAALVWWAASGAFLPPPLQQ
                     AWQLLHEPAAHSVHHAGPPVPARWSRAGDGSKLAHAPAPRKALCERGVSRGILVDWSA
                     RGDC"
     gene            1693..>2036
                     /locus_tag="VFN42_08775"
     CDS             1693..>2036
                     /locus_tag="VFN42_08775"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HET8996749.1"
                     /translation="METHVASEATTIASGGGLADRYASALYAHAEDTKSLDAVVAEMQ
                     GLGQVIDRSADFRRLLESPLIDVQTATRAATAVLEQEGFSKPVRDFVGVVTANRRLGA
                     LREIVQAFAALVA"
BASE COUNT          349 a          677 c          709 g          301 t
ORIGIN      
        1 gaccatgtct tcatgccgtg ttcccgccat ctgtggctgc atgccggttc ggtatgatcg
       61 ggccggcgta gcggccgcgg gtgcggtgtg cacgaaccgt gccagcgacg gctgaacagg
      121 ccggtcgccc gggcggccaa gccgccgggc tggcggtgcc cggttgggcg tgcggcggtt
      181 gccggttttt aagggaaatc gctaccagtt ggcccgttat cggggataag aggcggtccc
      241 cttctcacgg atccgtgatg catttcacaa gtcaaaaact gatccgccgg ctgctgccgg
      301 tgatgtcggg cgccggctgc atgatcgcac tgctgctggc aatgaacgtc atcgctgact
      361 ggaagaacca ggcgccgcgt atcggcgacc tgatcgcgtt cgagccggca cgggatgcgc
      421 tgctgccgga gtcggtgcgg atcgccgtcg aaacatccag tggtggccat tgtgtgctgg
      481 acgcgtacac gctgcggcgg tccggcggca gcttcatggt cagccgcggg ttggcgggcg
      541 cggaggcacg ctacatcgtc cattgggccg gcgcgcggac ggcggaaggg accgccagct
      601 gcggcgacag cgccgacctg atcctgacca ggcggcagct cgacaggctc ggcgaagcgg
      661 ccgcggcggg tggtatccgc caagcggcgg cagccaaggt cgtcagcagt ccccgcgggc
      721 cgaccagtcg accagaatcc cccggctgac gccacgttcg cacagggctt tccgcggcgc
      781 cggggcatgc gccagcttgc tgccatcacc ggccctggac cagcgcgccg gcaccggtgg
      841 cccagcgtgg tgaacggaat gcgccgccgg ttcatgcagc aactgccagg cctgctgcaa
      901 cggcggcggc agaaacgctc ccgacgccgc ccaccatacc agggcggcca ccaccagcgg
      961 ctccgcctgt gaaccggatt tgcagagcgg cagcgaccaa gtgcgccgca ccggccaggc
     1021 aagacgaagc ccgttcggcg tcagcatgtc ggcggcgagg tgggaggcat agccgacggc
     1081 gaccgcatcg gtggcccagc gcggcgcacc gccttgcagc agcacccagg cacaggccgc
     1141 caccgccagg gccgaatgcg tgatgccgcg atggccgaaa atagccgaga caagcatgga
     1201 caccgggcgc agacggctgc cgacccagga tttcggatga tcgatgtccg gcagcaggct
     1261 ggcagcggcg gcgagcgcga ttccggcggg gtcggccggt ccgaggccca gatgcggcga
     1321 ggccgcaagc caggccgccg cgccgagtgc cacatgggag ccggccatca tcggcgaaca
     1381 ttccttgcga tcctgataga gacggcgact cgggtcgccg cgcaacgtgc tacagcgcct
     1441 tcataacatt accagaagcc atgggcttgt ggcgtgggca tggcaagcga aaccgcaaaa
     1501 ccggcgatgc agcgggccac gtcggggatg gtcatcgggg atggtcgtcg gggatggtca
     1561 aaaatgaccc agacgtcata atccctccct accccgtgtt gcacaccccc cacccccatg
     1621 ctagacgcgc cgcgccggtc cgaccggcta tccctgtatc ccgctcggct ccctccaggg
     1681 ccagcacagc atatggaaac ccacgtggct tctgaggcta cgacaatcgc atccggcggc
     1741 ggcctcgccg atcgctacgc gtccgccctg tacgcgcacg ccgaggacac gaaatcgctt
     1801 gatgcggtcg tggccgaaat gcagggtctc ggacaggtga tcgaccgcag cgccgatttc
     1861 cggcgcctgc tcgaatcgcc attgatcgac gtgcaaacgg ccacgagggc cgcaacggcg
     1921 gtgttggagc aggaaggctt cagcaagccg gtgcgtgact tcgttggcgt ggtgacagcg
     1981 aaccgccgcc ttggcgccct gcgggagatt gtgcaggctt tcgccgccct ggtggc
//
LOCUS       DASSZH010000404         3854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1253892, whole genome shotgun sequence.
ACCESSION   DASSZH010000404 DASSZH010000000
VERSION     DASSZH010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253892"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /gene="metZ"
                     /locus_tag="VFN42_08780"
     CDS             complement(<1..1153)
                     /gene="metZ"
                     /locus_tag="VFN42_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HET8996750.1"
                     /translation="MSHPSQYRPATSLVHGGVQRTPYGETAEALFLTSGYVYDSAEQA
                     EATFAGTVEHYQYSRFANPTLTMLEERLALIEGAEACRTTATGMAAVNAALLAHLKAG
                     DRVVASRALFGSCHWIVSTLLPKFGITSEFVDGGDLDQWRAALRKPAQLVLLETPSNP
                     MLEIVDLRAVSELAHATGAIVVVDNVFATPLLQQPLQLGADVVVYSCTKHIDGQGRVL
                     GGAVLSSTKWINDVLQPFIRNTGPALSAFNAWLLLKGLETLKLRVEAGCRSAAALADF
                     LAGQSGVSRVWYPTRADHPQRALAMTQMTAGGTVVTFEVAGGKDAAFRVMNAFRLIAV
                     SNNLGDSKSLATHPATTTHMRIGVDERAKLGITDGVIRLSVGLEDVADLR"
     regulatory      complement(1206..1285)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            1381..1454
                     /locus_tag="VFN42_08785"
     tRNA            1381..1454
                     /locus_tag="VFN42_08785"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1413..1415,aa:Gly,seq:ccc)
     gene            1573..>3854
                     /locus_tag="VFN42_08790"
     CDS             1573..>3854
                     /locus_tag="VFN42_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HET8996751.1"
                     /translation="MATGGKWQFWIDRGGTFTDIVARDPSGHLSTHKLLSENPARYRD
                     AAIAGIKSILGLEPGAPIPPGIIDAVKMGTTVATNALLERKGERTLLVVNRGFADALR
                     IGNQARPRLFDLAISLPSMLYEEVVEIPGRVGVDGEAIEPLDETAARHAFSAARRKGI
                     TACAIVLMHAWKYPEHEQRLAELAREAGFAQVSASHAVSPLLRLVPRGDTTVVDAYLS
                     PILRRYVDQVAGELGGVRLYFMQSNGGLAEAGAFQGKDAILSGPAGGIVGAARTAAMA
                     GIERIIGFDMGGTSTDVALYDGKFERAFETAVAGVRMRAPMMAINTVAAGGGSILHFD
                     GARLRVGPDSAGADPGPACYRNGGPLTVTDANLCVGKIQPAHFPPIFGPNGDRKLDAD
                     IVRQKFAALAEEIAAATGERRTPQQVADGFLRIAVANMANAIKQVSVQKGHDAARFAL
                     QCFGGAGGQHACQVADALGMETVFIHPYAGVLSAYGMGLADQTAMREQAVEIPLEPHA
                     MGDLADLADRLASDAEAALVAQGADPARVFASRQLHLRYAGTEAALIIPLRDHATVVE
                     DFTAAHRARFGFATPERALVVEAVAVEAVSPGESIAEAEVAARSDGAAPPIDTVGIFT
                     NGAEHQAPVFERTALQAGDRIAGPALIREANATTVVEPGWTAEITKLDHMLLHRTEPL
                     ATRVPAGSDRPDPVLLELFNNLFMNVAEQTGAVLQNTSQSVNIKERLDFSCAIFDAAG
                     NLVANAPHVPVHLGAMGESVR"
BASE COUNT          611 a         1351 c         1305 g          587 t
ORIGIN      
        1 cgcgcaggtc cgccacatcc tccagcccga ccgacaggcg gatcacccca tcggtgatgc
       61 ccagcttcgc gcgctcgtcg acgccgatgc gcatatgcgt ggtggtggcc gggtgcgtcg
      121 ccagcgactt cgaatcgccc aggttgttgg ataccgcgat cagccggaag gcgttcatca
      181 cccggaacgc cgcgtccttg ccgccggcca cctcgaatgt caccaccgtg ccgcccgcgg
      241 tcatctgcgt catcgccagc gcgcgctgcg gatgatccgc gcgggtcgga taccacaccc
      301 ggctcacccc cgactgcccg gccaggaaat ccgccagcgc ggcggcgctg cggcagccgg
      361 cttcgacccg cagcttcagc gtctccagac ccttcagcag cagccaggcg ttgaacgccg
      421 acagcgccgg gccggtgttg cggatgaaag gctgcagcac gtcgttgatc catttcgtgc
      481 tggacagcac cgcgccgccc agcacgcggc cctggccgtc gatgtgcttg gtgcaggaat
      541 acaccaccac gtcggcgccg agttgcagcg gctgctgcag cagcggcgtg gcgaacacgt
      601 tgtccaccac cacgatcgcg cccgtcgcat gcgccagctc gctcaccgcg cgcagatcga
      661 cgatctccag catcggattg gacggcgttt ccagcagcac aagctgcgcc ggcttgcgca
      721 gcgccgctcg ccactggtcc agatcgccgc cgtcgacgaa ttcgctggtg atgccgaatt
      781 tcggcagcag ggtggagacg atccagtggc aggagccgaa cagcgcgcgc gacgccacca
      841 cccggtcacc ggccttcaga tgcgccagca gcgcggcatt gaccgcggcc atgccggtgg
      901 cggtggtgcg gcaggcttcc gcgccctcga tcagcgccag ccgctcctcc agcatggtga
      961 gcgtgggatt ggcgaagcgg gaatactggt agtgctcgac ggtgccggcg aaggtcgcct
     1021 ccgcctgttc ggcgctgtcg tagacgtagc cggaggtgag gaacagcgcc tcggcggtct
     1081 cgccataggg ggtgcgctgc acgccgccat gcaccaggct ggtggcggga cggtactggc
     1141 tggggtggga catgtgtctg ggcccttatc aacagttggc ccgaccgatg gagcagctag
     1201 agcccgatgg ctgttcgctg gcctctttag cgcgcttatt tcacctcgcc gcaatccggc
     1261 cggacaaatc acgagggcag gcccggcggt ggatcgttcc gccgcgaacc cgcggcggca
     1321 aaatagggcg gcgcggattg caccgtcaac ccggcttgcg tatgatccag aacgcccggc
     1381 gcgggtgtag ttcaatggta gaacggcagc ttcccaagct gcacacgagg gttcgattcc
     1441 cttcacccgc tccactgccg cgccctgcaa agcgtgcggc gcggcgaaca gcggcgatac
     1501 cccagccgga aagggcttgc gccccgcgtc actccgttgt gtctgtccct tgcagcgacc
     1561 aggaggtggg gcatggcgac cggcggcaag tggcagttct ggatcgaccg cggcggcacc
     1621 ttcaccgaca tcgtggcgcg tgacccttcc ggccacctca gcacccacaa gctgctgtcg
     1681 gagaacccgg cgcgctaccg tgatgccgcc atcgccggca tcaagagcat cctcggcctg
     1741 gaacccggcg cgccgatccc gcccggcatc atcgatgcgg tgaagatggg caccacggtg
     1801 gcgaccaacg ccctgctgga gcgcaagggc gagcgcacgc tgctggtggt caatcgcggc
     1861 tttgccgacg cgctgcgcat cggcaaccag gcgcggccac ggctgttcga tctcgccatc
     1921 tcgctgccct cgatgctgta cgaggaggtg gtggaaatcc ccggccgcgt cggcgtggac
     1981 ggcgaggcga tcgagccgct ggacgaaacc gccgctcggc acgccttcag cgccgcccgc
     2041 cgcaagggca tcaccgcctg tgccatcgtg ctgatgcacg cctggaaata cccggagcac
     2101 gagcagcgcc tggccgagct ggcgcgcgag gccggattcg cccaggtctc cgccagccat
     2161 gcggtcagcc cgctgctgcg gctggtgccg cgtggcgaca ccaccgtggt ggacgcctat
     2221 ctctcgccga tcctgcgccg ctacgtcgat caggtcgccg gcgaactggg cggggtgcgg
     2281 ctgtatttca tgcagtcgaa cggcgggctg gcggaagccg gtgcgttcca gggcaaggac
     2341 gccatcctgt cagggccggc cggcggcatc gtcggcgcgg cgcgcaccgc cgcgatggcc
     2401 ggcatcgagc ggatcatcgg cttcgacatg ggcggcacct ccaccgatgt cgcgctgtac
     2461 gacggcaagt tcgagcgcgc cttcgaaacc gcggtggccg gcgtgcgcat gcgtgcaccg
     2521 atgatggcga tcaacaccgt ggcggccggc ggcggctcca tcctgcattt cgacggtgcg
     2581 cggctgcgcg tcggaccgga cagtgccggt gccgatccgg ggccggcctg ctaccgcaat
     2641 ggcgggccgc tgaccgtcac cgacgccaat ctgtgcgtgg gcaagatcca gccggcgcat
     2701 ttcccgccga tcttcggccc aaatggcgac cggaagttgg acgccgacat cgtccggcag
     2761 aagttcgcgg cactggccga agagatcgcc gccgccacgg gggaaaggcg cacgccgcag
     2821 caggtggcgg acggtttcct gcgcatcgcc gtcgccaaca tggccaacgc gatcaagcag
     2881 gtttcagtgc agaagggcca cgacgccgcc cgcttcgcgc tgcaatgctt cggcggcgcc
     2941 ggtggccagc atgcctgcca ggtggccgat gcgctgggca tggagacggt gttcattcac
     3001 ccctatgccg gcgtgctgtc cgcctacggc atgggtctcg ccgatcagac cgcgatgcgc
     3061 gagcaggcgg tggagatccc gctggaaccg cacgccatgg gcgatctggc agacctggcc
     3121 gaccggctgg cctccgatgc cgaagccgcg ctggtggcgc aaggcgccga tcccgcccgg
     3181 gtgtttgcct cccggcagtt gcatctgcgc tatgccggca ccgaagccgc gctgatcatc
     3241 ccgctgcgcg accacgcgac cgtggtggag gacttcaccg ccgcacaccg tgcccgcttt
     3301 ggcttcgcca cgccggaacg cgcgctggtg gtcgaagccg tcgcggtcga agcggtctct
     3361 cccggcgaaa gcattgccga agccgaggtg gccgcccgca gcgacggcgc agccccgccg
     3421 atcgacacgg tcggcatctt caccaacggg gcggagcacc aagcgccggt gttcgagcgc
     3481 accgccttgc aggctggcga ccgcatcgcc ggtccggcgc tgatccgcga agccaatgcc
     3541 accaccgtgg tggagcccgg ctggacggcg gagatcacca agctcgacca catgctgctg
     3601 catcgcaccg aaccgctcgc cacccgcgtt cccgccggca gcgaccggcc ggatccggtg
     3661 ctgctggagc tgttcaacaa cctgttcatg aacgtcgccg agcagaccgg cgcggtgctg
     3721 cagaacacct cacagagcgt gaacatcaag gagcggctgg atttttcctg cgccatcttc
     3781 gacgctgccg gcaatctggt ggccaacgcg ccgcatgtgc cggtgcatct cggcgcgatg
     3841 ggcgaaagtg tgcg
//
LOCUS       DASSZH010000405         3602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1458684, whole genome shotgun sequence.
ACCESSION   DASSZH010000405 DASSZH010000000
VERSION     DASSZH010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458684"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VFN42_08795"
     CDS             complement(<1..401)
                     /locus_tag="VFN42_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003629120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HET8996752.1"
                     /translation="MADATQFLPGGGVVHLHRHDLPDGLTLGPVVAVDTETMGLDPRR
                     DRLCLVQLSGGDGNAHLVQIVPAALGGRGADCPNLKALLGDPAVLKLFHFARFDVAVM
                     QHYLGITVSPVKCTKIAAKLVRTFTDRHGLK"
     gene            complement(407..493)
                     /locus_tag="VFN42_08800"
     tRNA            complement(407..493)
                     /locus_tag="VFN42_08800"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(457..459),aa:Leu,seq:cag)
     gene            608..1558
                     /locus_tag="VFN42_08805"
     CDS             608..1558
                     /locus_tag="VFN42_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HET8996753.1"
                     /translation="MATRSVATVFGGSGFIGRYVVKRLAEAGHVVRVAVRDTERARQL
                     CPMGTVGQIVPLFTTLAERGTIERAVDGAEWVVNLVGILAERHHGDFQRVQAEGAGLI
                     GVAAAAAGVSRLVHVSAIGADPNAQSVYARTKGLGEQALRAAFPQATILRPSLVFGPE
                     DQFFNRFGALAALSPVMPVIAGATRMQPVYVGDVADAAMAGLTRADAAGRTYELGGPK
                     VWTFREILAWILQQTHRHRMMLNVPMGVARLQAAIMEKLPNKPLTRDQLLLLAKDNVV
                     AEGALGLRDLGITPTPVELVVPHYLHRFRPGGRHREEYTE"
     gene            1734..3170
                     /locus_tag="VFN42_08810"
     CDS             1734..3170
                     /locus_tag="VFN42_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8996754.1"
                     /translation="MPERMLQFVRVKQQTPDKRDAAVRRADFREIYDDFDPARAATQA
                     SRCSQCGVPFCQVHCPLQNNIPDWLKLTAEGRLEEAYEVSSATNTFPEICGRICPQDR
                     LCEGNCVIEKGYDSVTIGAVERYITDTAFARGWVKPGRPRHENGLSVGIIGAGPGGLA
                     AAEQLRRQGYAVHVYDRYDRVGGLMIYGIPNFKLEKEIVLRRWKQFEDAGVHFNLGVA
                     VGQDVTLAELRQRHDSVLIATGVYKARDIGGPGVGLPGIVRALDYLTASNRENLGEIV
                     PDFASGELNAAGKHVVVIGGGDTAMDCVRTAVRQGAASVKCLYRRDRANMPGSMREVK
                     NAEEEGVEFVWLSAPEAFLGDDRVAAVRAVRMRLGLPDATGRQAVEPVEGGHFTLGAD
                     LVIKALGFDPEDLPGAFNEPALNVTRWGTLKIDHRSFMTSLPGVFAAGDIVRGASLVV
                     WAIRDGRDAAREMHAWMRANATVPAAAE"
     gene            3176..>3602
                     /locus_tag="VFN42_08815"
     CDS             3176..>3602
                     /locus_tag="VFN42_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996755.1"
                     /translation="MQEDFVAAWAANAERLRHTYDPSQEHDACGVGLVAALDGKKRRD
                     VVQAGIDALRAVWHRGAVDADGKTGDGAGIHIEIPQVFFAEAIERGGHRVQPGGIGVG
                     MVFLPKTDFGAQERCRQIVESEILAFGYQIYGWRQVPINV"
BASE COUNT          583 a         1151 c         1293 g          575 t
ORIGIN      
        1 tccttcaacc catgcctgtc ggtaaaggtg cgcaccagct tcgcggcaat tttggtgcat
       61 ttcaccgggc tgaccgtgat gcccaggtaa tgctgcatca ccgccacgtc gaaccgggcg
      121 aagtggaaca gcttcagcac cgccggatcg cccagcagtg ccttcaggtt cgggcaatcg
      181 gcgccgcggc cgccgagcgc tgccgggacg atctgcacca gatgcgcatt gccgtcgccg
      241 cccgacaact gcacgaggca cagccggtcg cggcgcggat cgagacccat cgtctcggta
      301 tcgacggcca ccaccgggcc gagcgtcaat ccgtccggca ggtcatggcg gtgcagatgc
      361 accacgccgc cgccggggag aaactgagtt gcgtctgcca tcacgttggt gcccaggaga
      421 agactcgaac ttccacgacc ttgcggccac aggtacctga aacctgcgcg tctaccaatt
      481 ccgccacctg ggcaatggtc cgtgcggagg ccggcgattt agcgatggca cggggcggcg
      541 tcaactgccc ttgagagcgg cgccgcccgc ccatatcaac gccccggttc cggcaggaga
      601 gcgggcgatg gcgacgcgca gcgtggcgac ggtgttcggg ggctcggggt tcatcgggcg
      661 ctatgtggtc aagcggctgg cagaggccgg ccatgtggtg cgggtcgcgg tgcgcgatac
      721 cgaacgggcg cggcagcttt gcccgatggg cacggtcggg cagatcgtgc cgttgttcac
      781 cacgctggcc gagcgcggca cgatcgagcg cgcggtggac ggcgccgaat gggtggtgaa
      841 cctggtcggc atcctggccg agcggcacca cggcgacttc cagcgggtgc aggccgaggg
      901 ggcgggcctg atcggcgtcg ccgccgccgc cgccggggtg agccggctgg tgcatgtctc
      961 ggcgatcggc gccgatccga acgcgcagag cgtctatgca cgaaccaagg ggctgggcga
     1021 gcaggcgctg cgcgccgcgt tcccgcaggc gaccatcctg cgcccgtcgc tggtgttcgg
     1081 gccggaagac cagttcttca accgcttcgg cgcgctggcc gcgctgtcgc ccgtcatgcc
     1141 ggtgatcgcg ggcgccacac ggatgcaacc ggtctatgtc ggcgatgtcg ccgatgcggc
     1201 gatggcgggg ctgacgcgcg ccgacgcggc cgggcgtacc tatgagctcg gcgggccgaa
     1261 ggtctggacc ttccgcgaaa tcctggcgtg gattctccag cagacgcacc ggcaccggat
     1321 gatgctgaac gtgccgatgg gggtggcgcg gttgcaggcg gcgatcatgg agaagctgcc
     1381 gaacaagccg ctgacccgcg accagcttct gttgctggcg aaggacaatg tggtggcgga
     1441 gggggcgctg ggcctccgcg acctcggcat cacgccgaca ccggtcgaac tggtggtgcc
     1501 gcattatctg catcgcttcc ggccgggagg gaggcaccgg gaggagtaca cggaataata
     1561 gggccgaatc ggaccgatcc ttccgctggc ttctgcatgg ctgtcctgca aatgcccgaa
     1621 atgaccgcga attagggcag caattctgac ctgacggcaa attcgtgctc gcaagggcat
     1681 gcagactgtc ctatttaccg ggctgtaagg tggtccgaag ggggagtctg tcgatgcccg
     1741 agcgcatgct gcaattcgtc cgcgtcaagc agcagacgcc ggacaagcgt gacgccgccg
     1801 tccgccgcgc cgatttccgc gagatctacg acgatttcga cccggcccgc gccgcgaccc
     1861 aggccagccg ctgttcccag tgcggcgtgc cgttctgcca ggtgcattgc ccgctgcaga
     1921 acaacatccc ggactggctg aagctgacgg cggaaggacg gctggaggag gcgtacgagg
     1981 tctcctcggc caccaacacc ttccccgaga tctgcggccg catctgtccc caggaccggc
     2041 tgtgcgaagg caactgcgtg atcgagaagg gctatgacag cgtcaccatc ggcgcggtcg
     2101 aacgctacat cacggatacc gccttcgcgc gcggctgggt gaagcctggc aggccgcggc
     2161 acgagaacgg cctgtcggtc ggcatcatcg gcgccgggcc gggggggctt gcggcggcgg
     2221 agcagttgcg gcggcagggt tacgcggtgc atgtctatga ccgctacgac cgcgtcggcg
     2281 ggctgatgat ctacggcatc ccgaacttca agctggagaa ggagatcgtc ctccgccgct
     2341 ggaagcagtt cgaggacgca ggcgtgcatt ttaatcttgg cgtggcggtt gggcaagatg
     2401 tgacgttggc cgagttgcgg cagcgccacg attccgtgct gatcgccacc ggcgtctaca
     2461 aggcgcgcga catcggcggg ccgggcgtgg ggttgccggg aatcgtcagg gcgctggatt
     2521 acctcaccgc ctccaaccgc gagaacctgg gcgagatcgt gccggatttc gcctccggcg
     2581 aactgaacgc cgcgggcaag catgtggtgg tgatcggcgg cggcgacacc gcgatggact
     2641 gcgtgcgcac cgcggtgcgc cagggcgcgg ccagcgtgaa atgcctctat cgccgcgacc
     2701 gcgccaacat gcccggctcg atgcgcgagg tgaagaacgc cgaggaggag ggcgtcgagt
     2761 tcgtctggct ctccgcaccg gaagcgttcc tgggcgacga ccgcgtggcc gcggtgcgtg
     2821 ccgtgcggat gcggctcggc ctgcccgacg cgaccgggcg gcaggcggtg gagccggtcg
     2881 agggcgggca tttcactctc ggcgcagatc tggtcatcaa ggcgctgggt ttcgacccgg
     2941 aggatctgcc aggggcgttc aatgagccgg cgctgaatgt cacgcgctgg ggcacgctga
     3001 agatcgacca tcgcagcttc atgaccagcc tgcccggcgt gttcgccgcc ggcgacatcg
     3061 tgcgcggcgc ctcgctcgtc gtctgggcga tccgcgacgg gcgcgatgcc gcccgcgaga
     3121 tgcacgcctg gatgcgtgcc aacgccaccg ttcccgctgc cgcggagtga gccagatgca
     3181 ggaagatttc gtcgccgcgt gggccgcaaa cgccgagcgt ctgcggcaca cctatgatcc
     3241 gtcgcaggag catgacgcct gtggcgttgg acttgttgca gccctggacg gcaagaagcg
     3301 ccgcgacgtg gtgcaggccg gcatcgacgc gttgcgcgcg gtttggcacc gcggcgccgt
     3361 cgatgccgac ggcaagaccg gcgacggcgc cggcatccat atcgaaatcc cgcaggtgtt
     3421 cttcgccgaa gcgatcgagc gcggcggcca tcgcgtccag ccgggcggca tcggcgtcgg
     3481 catggtgttc ctgccgaaga ccgacttcgg tgcgcaggaa cgctgccggc agatcgtgga
     3541 atccgagatc ctcgcctttg gctatcagat ctatggctgg cggcaggtgc cgatcaatgt
     3601 cg
//
LOCUS       DASSZH010000406         2451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_742613, whole genome shotgun sequence.
ACCESSION   DASSZH010000406 DASSZH010000000
VERSION     DASSZH010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742613"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VFN42_08820"
     CDS             complement(<1..192)
                     /locus_tag="VFN42_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996756.1"
                     /translation="MPDNVIALRSRLLAREAEHAAEHEQQTSELQLREELKTGDAVSA
                     RTEQVHVRNGCSRTWCCGST"
     gene            complement(253..2337)
                     /locus_tag="VFN42_08825"
     CDS             complement(253..2337)
                     /locus_tag="VFN42_08825"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8996757.1"
                     /translation="MPPPVIAVEDVTRTFHIGDVDVQALRGVSLTVQAGEFVAIMGSS
                     GSGKSTLMAILGCLDRPSGGRYLLEGVDVSRLKEPQLAQIRSERLGFVFQSFNLLGRT
                     SAIENVALPLFYAPEGPVRPADRFARARQALALLGLAERERSTPGQLSGGQQQRVAIA
                     RALINAPGLLLADEPTGNLDSRTSHEIMQTLVALNRERGVTIVLVTHEADIAAYADRV
                     ITMRDGTIIADQRAVPPELAAVRHPATVAAGQSNAHPVGAAGAFALMIVSAAMQALNR
                     NRMRSALTMLGVFIGVAALIAMVAIGQGANQAVRKQIEGLGTNLLVVLPGATTTGGVR
                     AGFGSASTLTVQDAQAIRAEASAVSRVGYLIRGLGQVQYGGQNWTTSIQGVSPSYPPM
                     TNWEIASGRSISRDDDARSALVAVIGQTVARQLFNAGDNPVGAVIQVKGAPLHVVGLL
                     AGKGQTPFGQDQDDIVLIPFSTAERRVLGVAAPLQQQAPLNWAYPTWPNPYGLQLRLA
                     GYVNQVFVQAASPEAVDAAIRQVTQTLSERHRIKPGGIQDFSVRNLSQIAAAAEGSSR
                     IMALLLAAVASISLLVGGIGIMNILLVSVTERTREIGLRMAIGARRLHVLLQFLAEAV
                     FLSVTGGIAGILLGLGFSWGITLVAGWPAPVSPLAIAGGFLFSAAVGIFFGFYPARKA
                     ARLDPIEALRYE"
BASE COUNT          389 a          867 c          827 g          368 t
ORIGIN      
        1 agttgaccca caacaccatg tccgcgaaca accgttccgg acatggactt gctctgtccg
       61 cgcggagacc gcgtcgcccg tcttcaactc ctcgcgcagc tgcagttcgc tcgtctgctg
      121 ctcgtgttca gccgcgtgct ccgcttcccg cgccaacaac cgactgcgca acgcgatcac
      181 gttgtccggc atcactgcgg tggcgagtgg gacagtaccg gccagacaaa atggccgcgc
      241 ttcaccacga gcctactcgt agcgcagcgc ctcgatcgga tcgagccgcg ccgccttccg
      301 cgccgggtag aacccaaaga agatccccac cgcggcggag aacaggaatc cgccggcgat
      361 cgccagcggc gacaccggcg cgggccagcc cgccaccagc gtgatgcccc aggagaaacc
      421 cagtcccagc aggatgccgg caatgccgcc ggtgacgctg aggaacaccg cctcggccag
      481 gaactgcaac aacacgtgca gccgccgcgc accgatcgcc atgcgcaggc cgatctcacg
      541 cgtgcgctcg gtgaccgaca ccagcaggat gttcataatg ccgatcccgc cgaccagcag
      601 cgagatcgac gccaccgcgg ccagcaacag cgccatgatg cggctgctgc cttccgccgc
      661 cgcggcgatc tggctgaggt tgcgcaccga gaaatcctgg atgccacccg gcttgatgcg
      721 atggcgctcg gacaaggtct gcgtcacctg gcggatggcc gcatccactg cctccggact
      781 cgcggcctgg acaaacacct ggttgacgta tccggccagg cgcaattgca agccgtaggg
      841 attgggccag gtcggatacg cccagttcag cggtgcctgc tgctgcaacg gcgccgccac
      901 gcccagcacg cggcgctccg cggtgctgaa cgggatcagc acgatgtcgt cctggtcctg
      961 cccgaacggc gtctgtccct tgccggcgag caaccccacc acgtgcagcg gcgcgccctt
     1021 cacctgaatc accgcgccaa ccgggttgtc gcccgcgttg aacagctggc gggcgacggt
     1081 ctggccaatc accgcgacca gcgccgagcg ggcgtcgtcg tcgcgggaga tcgaacggcc
     1141 gctggcgatc tcccagttcg tcatcggcgg atagctcggg ctgacgccct ggatgctggt
     1201 ggtccagttc tgcccgccgt actgcacctg gcccaggccg cggatcaggt agcccacccg
     1261 gctgaccgcg gaagcctcgg cccggatcgc ctgcgcgtcc tgcaccgtga gcgtggaggc
     1321 gctgccgaac ccggcgcgca caccgccggt ggtggtggcg cctggcagca ccaccagcag
     1381 gttggtgccc aggccttcga tctgcttgcg cactgcctgg ttggcgccct gcccgatcgc
     1441 caccatggcg atcagcgccg cgacgccgat gaacacgccc agcatggtca gcgccgatcg
     1501 catgcggttg cggttgagcg cctgcatcgc ggcgctgacg atcatcaggg cgaaggcccc
     1561 ggcggcaccg accggatgcg cgttgctctg acccgccgcc acggtggccg ggtgccgcac
     1621 cgccgccagt tccggcggca ccgcacgctg gtcggcgatg atggtgccgt cgcgcatggt
     1681 gatcacccgg tcggcgtagg cggcgatgtc cgcctcatgc gtgaccagca cgatggtgac
     1741 gccgcgttcg cggttgagcg ccaccagcgt ctgcatgatc tcatgcgagg tgcgggaatc
     1801 caggttgccg gtgggttcgt cggccagcag cagccccggc gcgttgatca gcgcacgggc
     1861 gatggcgacg cgctgctgct gtccgccgga caactgcccc ggcgtgctgc gctcgcgttc
     1921 ggcgaggccg agcagtgcca gtgcctggcg ggcacgggcg aagcggtcgg cggggcgcac
     1981 cggaccttcc ggcgcataga acagcggcag cgcgacattc tcgatcgcgc tggtgcggcc
     2041 aagcaggttg aagctttgga acacgaaacc caggcgctcg ctacggatct gcgcgagttg
     2101 cggttccttc agccgcgaga catcgacgcc ctccagcagg tagcggccgc cggatgggcg
     2161 gtcgaggcag cccaggatcg ccatcagcgt cgatttgccg gagccggacg atcccatgat
     2221 ggcgacgaac tctccggcct gcacggtcag gctgacgccg cgcagcgcct gcacgtccac
     2281 gtcgccgatg tggaacgtgc gggtgacatc ctcgacggcg atgacgggcg gaggcatggg
     2341 ccgttacagc cggggcagcc gcggggttgc gctgcgggat ggcgtgccgc gctgttcgcc
     2401 ggtgatcacc cgatcgccgg gctgcacggc gccttgcagg atttcggtgt t
//
LOCUS       DASSZH010000407         5707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_793737, whole genome shotgun sequence.
ACCESSION   DASSZH010000407 DASSZH010000000
VERSION     DASSZH010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793737"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..1081
                     /locus_tag="VFN42_08830"
     CDS             194..1081
                     /locus_tag="VFN42_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8996758.1"
                     /translation="MIMIFGIPGPLLFGQLLLGLINGAFYALLSLGLSVIFGMLNIIN
                     FAHGALFMMGAFAAWMLLNYVGIGYFPALIVAPLIVGAFGILLERTLIKQMYKLDHLY
                     GLLLTFGLALVIEGLFRNYYGSSGMPYQIPKVLQGATNLGFMYLPIYRGWVVVAALVV
                     CIATWAIIEKTPLGATLRAATENAPLVQAFGVDVPRVITLTFGGGVALAAFAGVLAAP
                     IYSVNPNMGDNFINTVFAVVVIGGMGSILGSIITGFGLGVIEGLTKVFYPQASATVVF
                     VVMVIVLLALPHGLFGKRA"
     gene            1083..2069
                     /locus_tag="VFN42_08835"
     CDS             1083..2069
                     /locus_tag="VFN42_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8996759.1"
                     /translation="MSELTRPAETARASRSNVPFGFSRPQWYAFLMLVALIVVCPYVL
                     YPIFLMRVLCIALFACAFNLMIGYVGLLSFGHAAFFGMGAYISAWTMKFMGVDAVVAI
                     LLAGLAGAALGLCLGYLAIRRSGIYFAMITLALAQMVYFFCVEAPFTGGEDGIQRVPR
                     GHLFGILNLNNDMTMYWVVAVIFLGGFLLIHRVIHSPFGQVLKAIRENEPRATSLGYR
                     TDHYKLIAFVLSCMLSGVAGGTKAIVFGIATLTDVHYSTSGDVVLTTLVGGLGTIFGP
                     VMGAVVVTAMENYLAEFGAWVTVTQGVIFVLCVLAFRRGIIGELGAKLRWSL"
     gene            2173..2817
                     /locus_tag="VFN42_08840"
     CDS             2173..2817
                     /locus_tag="VFN42_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996760.1"
                     /translation="MKRLLPAAIAALCLTSPAWGALTVYSHVGAWDVFSGTGTDGRAV
                     CGIGNSNTTNGRAFSLRYIRGADGITFAADKPSWNIPEGTHLQVVMQVGLNPPWTQDA
                     VGHGQRVEWTMEPRNVQVFDTQFRRAGSMTVTFPSGNEPPWVLSLSGSTAASNAMGRC
                     VTAMMRSSTTAAPGQSAPQGPTQPFASTPEAPSQEGTPSQPTQPNSPPPLPPTH"
     gene            2965..3933
                     /locus_tag="VFN42_08845"
     CDS             2965..3933
                     /locus_tag="VFN42_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007399965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET8996761.1"
                     /translation="MQLSHADLLKAYRSMVTIRRFEERVQEEFSKGGIPGFVHLYAGE
                     EASGVGVCSHLTPQDVIASTHRGHGHSLAKGCDVTGMMLELFGKQGGLCGGKGGSMHI
                     ADLHQGMLGANGIVGGGPPLVVGAALTAKTLGTRNVAVCFTGDGGSNQGTTFEALNMA
                     VVLRLPAVFVFENNGYGEYTSAKYAVGSGNIANRAAGFGMPAVKVNGDDFFAVHEAAR
                     EAIERARDGGGPSAIEVDTCRFYGHHSGDAQLYRGKDEVKRLREERDCLKHFRARVTE
                     AALLDAAELDAVDHEVADLIDQAVATARAAPPPDPATLLTDVYVSY"
     gene            3947..4978
                     /locus_tag="VFN42_08850"
     CDS             3947..4978
                     /locus_tag="VFN42_08850"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003622424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET8996762.1"
                     /translation="MTVKTIRQAISEALTQEMRRDPRVVIIGEDVSGGAGTEGGRDAA
                     GGVLGVTKGLIKEFGEHRVIDTPITESAIMGAAAGAAATGLRPVAELMFSDFFGVCFD
                     QIYNQAAKFRYMFGGKARTPLVIRTMVGAGRSAAAQHSQSPYHIFTSVPGLKVVVPSN
                     AYDAKGLLIQAIRDDDPVIFCEHKLMYDLRTEVPDEPYTIPFGEANIVRDGDDVTVVA
                     LARMVHYTAEAAETLAAEGIECEIIDPRTTSPLDEDTILESVERTGRLVVVDEATPRC
                     SMATDIAALVADKAFASLKAPVRRVTAPHTPVPFAPSLEKLYIPDPERIAAAVRAVCG
                     YARPGQAAA"
     gene            5079..>5707
                     /locus_tag="VFN42_08855"
     CDS             5079..>5707
                     /locus_tag="VFN42_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET8996763.1"
                     /translation="MSIELRRDERGVATVTIANAAKLNTLNPALMTKLVETVETLGRD
                     PALRVVILRGEGERAFIGGADITAMATLDAAGARAFITQVHRSCDVFRRLPVPVIARI
                     QGYTLGAGLEVAAACDFRVAAEDAVFGMPEVRIGLPSVVEAALLTGLIGWGRTRQLLL
                     TGENIDARKAEAWGFVEQVVPAAGLDSTVERLVQCILDSGPRAVRLQKTL"
BASE COUNT          895 a         1920 c         1897 g          995 t
ORIGIN      
        1 gggccgttat ggaaattgca gtgacgggtc gtccccgtac ctcgatgtcg tggccgggct
       61 tgacccgacc tcctaccccg ggtcgatcgg gtcgcgacgc atacgctcgg gcgggggaag
      121 atggccgggt caaacccggc catgacttcg ggacggagtt aagcaggccg cacgtacccc
      181 ggggagttct cgcatgatca tgattttcgg tatccccggc ccgctgctgt tcggccaatt
      241 gctgttgggc ctgatcaacg gcgccttcta cgccttgctg tcgctcggcc tgtcggtgat
      301 cttcggcatg ctcaacatca tcaacttcgc gcatggcgcg ctgttcatga tgggcgcgtt
      361 cgccgcctgg atgctgctga attacgtcgg catcggctat ttccccgccc tgatcgtcgc
      421 cccgctcatt gtcggcgcct tcggcatcct gctggagcgc acgctgatca agcagatgta
      481 caaactggac cacttgtacg gcctgttgct gaccttcggc ctggcgctgg tgatcgaggg
      541 cctgttccgc aactactacg gcagctccgg catgccgtac cagattccga aggtgctgca
      601 gggggccacc aacctgggct tcatgtacct gccgatctat cgcggctggg tcgtcgtcgc
      661 cgcgctggtc gtctgcattg ccacctgggc catcatcgag aagaccccgc tcggcgccac
      721 cctgcgggcc gcgacggaaa acgccccgct ggtgcaggcg ttcggcgtcg acgtgccgcg
      781 ggtcatcacg ctgaccttcg gcggcggcgt ggccctggcg gccttcgccg gcgtgctggc
      841 cgcgccgatc tactcggtga accccaacat gggcgacaac ttcatcaata ccgtgttcgc
      901 cgtggtggtg atcggcggca tgggctcgat cctgggatcc atcatcaccg gcttcggcct
      961 gggcgtgatc gagggactga ccaaggtctt ctatccccag gcctccgcca ccgtggtgtt
     1021 cgtggtgatg gtcatcgtgc tgcttgcatt gccgcacggt ctgttcggca agagggcctg
     1081 agatgtccga gcttacccgt ccggccgaaa ccgcccgcgc ctcgcgttca aacgtcccct
     1141 tcggcttctc gcgtccgcaa tggtacgcgt tcctgatgct ggtggcgctg atcgtggtct
     1201 gcccctacgt gctgtacccg atcttcctga tgcgcgtgct gtgcatcgcg ctgttcgcct
     1261 gcgccttcaa cctgatgatc ggctatgtcg gcctgctgtc cttcgggcat gccgccttct
     1321 tcggcatggg cgcctacatc tccgcctgga ccatgaagtt catgggcgtg gatgccgtgg
     1381 tcgccatcct gctggccggc ctcgcgggcg cagcgctcgg actgtgcctg ggctacctgg
     1441 cgatccgccg ctccggcatc tacttcgcca tgatcaccct ggcactggcg cagatggtgt
     1501 atttcttctg cgtcgaggca ccgttcaccg gcggcgagga cggtatccag cgggtgccgc
     1561 gcggccatct gttcggcatt ctgaacctga acaacgacat gaccatgtac tgggtggtcg
     1621 cggtcatctt cctgggcggg ttcctgttga tccaccgggt gatccactcg cccttcggcc
     1681 aggtgctgaa ggcgatccgg gagaacgagc ctcgtgccac ctcgctgggc taccggaccg
     1741 atcactataa actgatcgcc ttcgtactgt cctgcatgct gtccggcgtg gccggcggca
     1801 ccaaggcgat cgtgttcggc atcgccacac tgaccgacgt gcactactcc acctccggcg
     1861 acgtggtgct gaccacattg gtgggcggcc tgggcactat cttcgggccg gtcatgggcg
     1921 cggttgtcgt caccgccatg gaaaattacc tggccgagtt cggcgcctgg gtgacggtaa
     1981 cccagggcgt gatcttcgtg ctctgcgtgc tggctttccg ccgcggcatc atcggagagc
     2041 tcggcgccaa gctccgctgg tcgctgtaac ggcgtcccgc tccgcccctg cgcggaacgg
     2101 gaacggcgcc ggcccttgcc aatctccccc ggcagcgcca actcgatgcg catccgcata
     2161 gtggaggtcg agatgaagcg tctgttgccg gcggccatcg ccgcgctgtg cctgaccagc
     2221 cccgcctggg gagccttgac cgtctattcc catgtcggcg cctgggacgt gttcagcggc
     2281 accgggacgg acggacgggc ggtctgcggg atcggcaaca gcaacaccac aaacggccgg
     2341 gccttctcgc tgcgctatat ccggggagcc gacgggatca ccttcgccgc cgacaagccg
     2401 agctggaaca ttcccgaggg tacccacctg caggtggtga tgcaggtcgg actgaacccg
     2461 ccctggaccc aggacgcggt tggccacggc cagcgcgtgg agtggaccat ggagccccgc
     2521 aacgtgcagg tctttgacac ccaattccgc agagcgggct cgatgacggt gacctttccg
     2581 tcgggcaatg aaccgccctg ggtactcagc ctgagcggct cgaccgccgc gagcaatgcc
     2641 atgggccgct gcgtcaccgc gatgatgcgc agctcgacga cggccgcgcc ggggcaatcg
     2701 gcgccgcagg gaccaacgca gccattcgcg tcaacgccgg aggcgccctc acaggagggc
     2761 accccgagcc aacccaccca gccgaactcc ccgccgccgc ttcccccgac gcactgacgg
     2821 cgcgacgcgc gaccctcctg ctcgccgcgg cgctcgcggc gcgccgatac atgcccgggg
     2881 tgccaccgcg acagcggcgg cgccccgagc taatgttccg gcggcggccg gcctgccggc
     2941 ggcccacaat cagagggaag cgccatgcag ctcagccatg ccgatctgct caaagcctat
     3001 cgctccatgg tcaccatccg ccgcttcgag gagcgggtgc aggaggagtt ctccaagggc
     3061 ggtatccccg gcttcgtgca tctctatgcc ggtgaggaag cctccggggt gggggtctgt
     3121 tcgcacctca cgccgcagga tgtcatcgcc agcacgcatc gcgggcatgg ccacagcctg
     3181 gcgaaaggct gcgatgtcac cggcatgatg ctggagctgt tcggcaaaca gggcgggctg
     3241 tgtggcggca agggcggctc catgcacatc gccgatctgc accagggcat gctcggcgcc
     3301 aacggcattg tcggcggcgg gccgccattg gtggtcggcg ccgcgctgac cgcgaagacc
     3361 ctgggcacgc gcaacgtcgc ggtatgcttc accggcgacg gcgggtccaa ccagggcacc
     3421 acgttcgaag cactgaacat ggcggtggtg ctgcggcttc ccgcggtgtt cgtgttcgag
     3481 aacaacggct acggcgagta caccagtgcg aaatacgcgg tcggcagcgg caacatcgcc
     3541 aaccgcgccg cggggttcgg catgccggcc gtcaaagtga atggtgacga tttcttcgcg
     3601 gtgcatgaag ccgcgcgcga ggcaatcgaa cgcgcccgcg acggcggtgg gccgagcgcg
     3661 atcgaggtgg atacctgccg tttctacggc caccacagcg gcgacgcgca gctctatcgc
     3721 ggcaaggacg aggtgaagcg gctgcgtgag gagcgcgatt gcctgaagca cttccgtgcc
     3781 cgcgtgaccg aggcggcgct gctggatgcg gcggagctgg atgcggtgga tcatgaggtc
     3841 gcggacctga tcgaccaggc ggtggcaacc gcgcgcgctg cgccgccgcc cgatcccgcg
     3901 acactgctga ccgacgtgta cgtgtcgtat tgaagggggg cgtgccatga ccgtgaaaac
     3961 catccgccag gcgatcagcg aggccctgac ccaggaaatg cgccgcgacc cacgcgtggt
     4021 gatcatcggc gaggatgtgt ccgggggtgc cggcaccgaa ggcggccgcg acgccgcggg
     4081 cggcgtactg ggcgtcacca agggcctgat caaggaattc ggcgagcacc gcgtcatcga
     4141 tacgccgatc accgagtcgg cgatcatggg cgctgccgct ggtgccgccg cgacgggcct
     4201 gcggccggtg gcggagctga tgttctccga cttcttcggc gtctgcttcg accagatcta
     4261 taatcaggct gcgaagttcc gctacatgtt cggcggcaag gcacgcacgc cgctggtgat
     4321 ccgcaccatg gtcggggccg gccgcagcgc cgccgcgcag cattcgcaaa gcccgtacca
     4381 catcttcacc agcgtgcccg gactgaaggt ggtggtgccg tccaacgcgt acgatgcgaa
     4441 gggcctgctg atccaggcga tccgcgacga cgacccggtg atcttctgcg agcacaagct
     4501 gatgtacgac ctgcgcacgg aagtaccgga cgagccgtac accatcccct tcggcgaggc
     4561 gaatatcgtg cgcgacggcg atgacgtcac ggtcgtcgca ctcgcgcgca tggtgcacta
     4621 cacggccgaa gcggcggaaa cgctggcggc ggagggcatc gaatgcgaga tcatcgaccc
     4681 tcgcaccacc tcgccgctgg acgaagacac cattctggaa agcgtcgaac gaaccggccg
     4741 gctggtcgtg gtggatgaag cgacgccgcg ttgcagcatg gccaccgaca ttgcggcgct
     4801 ggtcgccgac aaggccttcg catcgctgaa ggccccggtc cgccgcgtca ccgcgccgca
     4861 cacgccagta ccgttcgcac cgtcgctgga gaagctgtac atccccgatc cggagcgcat
     4921 cgccgctgcg gtgcgcgcag tttgtggtta cgcaagaccg ggccaggcgg ctgcgtgagg
     4981 cagactccct tgacgcgcag caggggtggg ttttccccca ctgttgcgca gggcgcatgc
     5041 tgccggcccc gcctgcatcg ccaagaggaa gtgcctgcat gagcatcgaa ctccgccgtg
     5101 acgaacgcgg cgtggccacc gtgaccatcg ccaacgccgc caagctgaac acgctgaacc
     5161 ccgccctgat gaccaaactg gtcgaaacgg tggaaacgct cggccgcgat cctgcgctgc
     5221 gtgtcgtcat cctgcgcggc gagggcgaac gtgccttcat cggcggcgcc gacatcaccg
     5281 cgatggccac gctggatgct gccggcgcgc gcgccttcat cacccaggtg caccgtagct
     5341 gcgacgtgtt ccgccgcctg ccagttccgg tgatcgcccg catccagggc tataccttgg
     5401 gtgccggcct ggaggtcgcg gccgcctgcg acttccgcgt cgcggcggag gatgcggtgt
     5461 tcggcatgcc ggaggtgcgc atcggcctgc cgtccgtcgt ggaggccgcg ctgctgaccg
     5521 gcctgatcgg ctggggccgc acccggcaat tgctgctgac cggagagaat atcgatgcgc
     5581 gtaaggcgga agcctggggt ttcgtggaac aggtcgtccc cgccgcgggt ctggactcca
     5641 ccgtggagcg gctggtccag tgcatcctgg acagcggtcc gcgcgccgtc cggctgcaga
     5701 agacact
//
LOCUS       DASSZH010000408         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1594922, whole genome shotgun sequence.
ACCESSION   DASSZH010000408 DASSZH010000000
VERSION     DASSZH010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1594922"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VFN42_08860"
     CDS             complement(<1..332)
                     /locus_tag="VFN42_08860"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HET8996764.1"
                     /translation="MSEYSKLLPQPTADTQPYWDGLNAHKLRLQRCADCGKVRHYPRP
                     ICDACWSMRSEWFDASGRGHVHSWTVTHHAFHPGWKQELPYTLVTVDLEEGVRMNAPL
                     RRLDPSELR"
     gene            complement(335..1480)
                     /locus_tag="VFN42_08865"
     CDS             complement(335..1480)
                     /locus_tag="VFN42_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996765.1"
                     /translation="MSLREAGAVTGIGETEYMRGTDRTALSLQLEASLKAIADAGLKP
                     SDIDGLIPYGLGAVAEDLATAFGTRDLKFSATTPMGGASAVAAIQCAVAVVQAGICKH
                     VLLSLGRTGFSGGRIGARVQQMPQFRVIGEFEMPSGAIAPAQLYAVMARRHMELYGTT
                     SLQFAEIAVSTRLNASRNPNATMRKPITVEDHQASRMISDPLRLLDCSLESDGAAAVV
                     ISAAERARDMKQRPVLVMGVAEGHPDSPSAITQRPDITTLGLAKAAPRAFAMAGITPE
                     DVDVAEIYDCFTYNVLCQLEDAGFCKKGEGGPFVESGAIRMGGRLPVNTHGGLLSQAH
                     MAGMNHVVELVRQMRGEAAVQVPDAEIGVVTGYGDMGDGSIAIMRRA"
     gene            complement(1680..>2052)
                     /locus_tag="VFN42_08870"
     CDS             complement(1680..>2052)
                     /locus_tag="VFN42_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8996766.1"
                     /translation="ELPSGTGRRVIGFATAGRARRTQISGRRLGEGEIETLYVLDDWR
                     DRGVGRGLIRAAGAFLAQAGCRSAFLWVLRDNPSRWFYAHLGGTPAAEAMIHVGGTPI
                     AQTAFVWDPIEKLLAASPQKS"
BASE COUNT          310 a          718 c          685 g          339 t
ORIGIN      
        1 cgcagttccg acggatccag gcggcgcagc ggtgcgttca tccggacgcc ttcctccagg
       61 tccaccgtca ccagcgtgta gggcagctcc tgcttccagc ccggatggaa ggcgtggtgc
      121 gtcaccgtcc agctatgcac atgcccgcgg ccggaggcat cgaaccattc gctgcgcatc
      181 gaccagcacg catcgcatat cgggcgtgga taatgccgta ccttgccgca atcggcgcag
      241 cgctgcaggc gaagcttgtg cgcgttcagc ccgtcccagt agggctgcgt gtccgccgtg
      301 ggttgcggca gcagcttgct gtactcgctc atgctcaggc cctccgcatg atggcgatcg
      361 agccgtcgcc catgtcgcca tagccggtga ccacgccgat ctccgcatcc ggcacctgca
      421 ccgccgcttc cccgcgcatc tggcgcacca gctccaccac atgattcatc ccggccatat
      481 gcgcctgcga cagcagcccg ccatgcgtgt tcaccggcaa ccggccgccc atgcggatcg
      541 ctccggattc cacgaaggga ccgccttcgc ccttcttgca gaagccggca tcctccaact
      601 ggcacagcac gttgtaggtg aagcagtcgt agatctccgc cacatccaca tcttcgggcg
      661 tgatcccggc catggcgaat gcgcgcggcg ccgccttggc caggcccagc gtcgtgatat
      721 ccggccgctg ggtgatcgcg ctgggggaat ccggatggcc ttccgctacg cccatcacca
      781 gcacgggccg ctgcttcatg tcgcgtgcgc gttccgccgc gctgattacc accgccgccg
      841 cgccgtcgct ttccaggctg caatcgagca ggcgcagcgg atcggaaatc atgcgggaag
      901 cctggtggtc ctccaccgtg atcggcttgc gcatggtcgc gttcggattg cgcgaggcgt
      961 tgagccgcgt gctgaccgcg atctcggcga actgcagcga ggtggtgccg tacagttcca
     1021 tgtgccgccg cgccataacg gcataaagct gcgccggggc gatcgcacct gacggcatct
     1081 cgaactcgcc gatgacccgg aattgcggca tctgctgcac gcgcgcaccg atgcgcccgc
     1141 cggaaaaccc ggtgcgcccg agcgacagca gcacgtgctt gcagatgccg gcctgcacca
     1201 cggcgacggc gcattggatg gcggcgaccg cggaggcgcc gcccatcggc gtggtggcgg
     1261 agaatttcag gtcgcgcgtg ccgaaggccg tggcgaggtc ctcggcgacg gcgccgagcc
     1321 cgtaggggat cagcccgtcg atgtcggatg gtttgaggcc ggcatccgcg atggctttca
     1381 gcgatgcttc cagctgcaac gacagtgcgg ttcggtcggt gccgcgcatg tactcggttt
     1441 cgccgattcc ggtcacggcg ccggcctcgc gcaaggacat gacggttcct cctgttgtcg
     1501 ttcgatgccg atcgtgcggc gctggccggg gcggtcaagc cccatgcgcg tttagatagg
     1561 cgacgaggca gcgaaacgtg tcatggccgg ccttcgccgg ccatgacagg gggcgtgaac
     1621 cggctcacca ccgcaatttt ctaacgcctg gtttagcgcc tatgcggtca ggccccgggc
     1681 taggatttct gcggcgacgc cgccagcagc ttctcgatcg ggtcccagac gaaggcggtc
     1741 tgcgcgatcg gcgtgccgcc gacatggatc atcgcctcgg cggccggcgt gccgcccagg
     1801 tgggcataga accagcggct ggggttgtcg cgcagcaccc acaggaaggc gctgcggcag
     1861 ccggcctggg ccaggaaggc gccggcggcg cggatcagcc cgcgtcccac cccgcggtcg
     1921 cgccagtcgt ccagcacata aagcgtctcg atctccccct cgcccaggcg gcggccggag
     1981 atctgggtgc gccgggcgcg gccggcggtg gcgaagccga tcacccgccg gccggtgccc
     2041 gacggcagct cg
//
LOCUS       DASSZH010000409         2795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_163351, whole genome shotgun sequence.
ACCESSION   DASSZH010000409 DASSZH010000000
VERSION     DASSZH010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163351"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1479)
                     /locus_tag="VFN42_08875"
     CDS             complement(136..1479)
                     /locus_tag="VFN42_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8996767.1"
                     /translation="MARIEIIGGRTMNRAARKVLPRSLLGRSLLMILLPLILLQAVAL
                     QIFYGSHLDLVSRRLSGSVVSEIAFTLDMLNRFHDPKDRAWILRKAHEEYELVMTLDR
                     GARLPHHTPVNILGPMDDELAATLRFRFHSQFIADWTSNPDTVFIQLQLPDGVLHVAV
                     PRKRLYAGSFYIFVAWLAGVSALVFGIAALFMRNQVRAIRRLAGAAEAFGRGRDIGPI
                     KPEGAVEVRKAATAFNRMQERILRFLAQRTEMLAGVSHDLRTPLTRLRLALEVMPQTE
                     ANQQDVIGMKADVEEMDRMITGYLAFARGEGSEQAEEVNLSALLEEVAAGARRAGAEV
                     ALDVPPTLTLSLRGNAVRRAVTNLVDNARRHAKHIGLAALLQGRSVLVMVDDDGPGIP
                     PDRRESVFRPFESGAAGGTGLGLTIARDIVRAHGGEILLEDSPLGGLRAQIRLPV"
     gene            complement(1516..2214)
                     /locus_tag="VFN42_08880"
     CDS             complement(1516..2214)
                     /locus_tag="VFN42_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006557520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8996768.1"
                     /translation="MADEPHILVVDDDARLRGLLSRYLAGEGFRVSTAESAEDARAKL
                     RAINPDLLVLDVMMPGESGLTLTESLRRENGHDLPVLLLTARGAPEDRIAGFEAGADD
                     YLGKPFEPRELVLRVRALLRRIPPPPPESHAHPVPLGAAEFDPARGELRGPEGPIRLT
                     GGEAALLAALARKPNEVLSREDIAAALDMDETGERAIDVQVTRLRRKIEADPREPRFL
                     HTVRGRGYVLKPGT"
     gene            complement(2214..2681)
                     /locus_tag="VFN42_08885"
     CDS             complement(2214..2681)
                     /locus_tag="VFN42_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET8996769.1"
                     /translation="MRLAQDLLFFAYRDFTNAADVILEELGLGRAHHRALHYIGRNPG
                     ITVSDLLVILRITKQSLARVLSLLVEQGYVAQTPGREDRRQRLLTLTAKGHALERRAF
                     ERQRERLTLAYREAGPIAVDGFRRVMRSIMDQQARDYLDRTVPEMQPPRVRQA"
BASE COUNT          430 a          976 c          949 g          440 t
ORIGIN      
        1 accgacgcct tgctgcggga gactcccaaa ccgcggcttg cggcaatgag ccatccccaa
       61 caagtcgtgg gcggccgggc caggcccggc cacgacaaga aagctcgcgg ctgccgtgct
      121 tgcgtcagcc ctccgtcaca ccggcagccg gatctgcgcg cgcaggccgc ccagcgggct
      181 gtcctccagc aggatctcgc cgccatgcgc acgcacaatg tcgcgggcga tggtcaggcc
      241 caggccggtg ccgccggcgg ccccgctctc gaacgggcgg aacacgcttt cccgccggtc
      301 cggcgggatg cccgggccgt cgtcatcgac catcaccagc accgatcggc cctgcagcaa
      361 ggcggccagc ccgatatgct tggcatgccg ccgggcgttg tccaccaggt tggtcaccgc
      421 gcgccgcacg gcgttgccgc gcagcgacag tgtcagcgtc ggcggcacat ccagcgcgac
      481 ctccgctccc gcccgccgcg cgccggcggc gacttcttcc agcagggccg agagattgac
      541 ctcctcggcc tgttccgacc cttcgccgcg cgcgaaggcc agatagccgg tgatcatgcg
      601 gtccatctcc tcgacgtccg ctttcattcc gatcacgtcc tgctggttcg cttcggtttg
      661 cggcatcact tccagggcca gccgcagccg ggtcagcggc gtgcgcagat catgcgacac
      721 acccgcgagc atttcggttc gctgtgccag gaaccgcagg atgcgttcct gcatgcggtt
      781 gaacgccgtc gccgccttgc gcacctccac cgcgccttcc ggcttgatcg gaccgatgtc
      841 acggccgcgg ccgaatgcct ccgcggcgcc ggccagccgg cggatcgcgc gcacctggtt
      901 gcgcatgaac agcgccgcaa tgccgaacac cagcgccgac acgccggcca accatgcgac
      961 aaagatatag aaggagccgg catagagccg cttgcgcggt acggcgacgt gcagcacgcc
     1021 atccggcaac tgcaactgga tgaacaccgt atccgggttg gacgtccagt cggcgatgaa
     1081 ttgcgaatgg aagcggaagc gcagcgtagc ggccagttcg tcatccatcg gtcccaggat
     1141 gttcaccggc gtgtggtgcg gcagcctggc gccgcgatcc agcgtcatga ccagctcgta
     1201 ttcctcatgc gccttccgca ggatccaggc ccggtccttg ggatcgtgaa agcggttcag
     1261 catgtccagg gtgaaggcga tctcgctgac cacgctgccc gacagccggc gggacaccag
     1321 gtcgagatgg ctgccgtaga agatctgcaa tgcgaccgcc tgcagcagga tcagcggcag
     1381 caggatcatc agcaggctgc ggccgagcag cgaacgtggc agcactttgc gtgccgcgcg
     1441 gttcatggtg cggccaccga taatctcaat ccttgccatc acactcccgc aaggcttttt
     1501 ccggcgtggt gccggtcagg tgccgggttt tagcacatag ccgcggccgc ggactgtgtg
     1561 cagaaagcgc ggctcgcgcg ggtccgcctc gatcttgcgg cgcaggcggg ttacctgcac
     1621 gtcgatcgcg cgttcgccgg tttcgtccat gtccagcgcg gcggcgatgt cctcgcgcga
     1681 cagcacctcg ttcggcttgc gcgcgagcgc ggcgagcagc gccgcttcgc cgccggtcag
     1741 ccggatgggg ccttcgggtc cgcgcagttc gccgcgggcc gggtcgaact ccgcggcgcc
     1801 gagcggcacc ggatgggcat gcgactcagg cggtggcggg ggaatccggc gcagcagcgc
     1861 gcgcacccgc agcaccagct cgcgcggttc gaacggcttg cccaggtagt cgtcggcgcc
     1921 ggcctcgaag cccgcgatgc gatcctccgg cgcgccgcgt gcggtcagca gcagcaccgg
     1981 caagtcgtgc ccgttctccc gccgcagcga ttcggtgagg gtgaggccac tttcgcccgg
     2041 catcatcacg tccagcacca gcaggtccgg attgatcgcg cgcagcttgg cgcgggcatc
     2101 ttcggcgctc tccgccgtgc tcacgcggaa gccctcaccg gccagatagc gtgacagcag
     2161 cccgcgcagc cgcgcatcgt catccaccac caggatgtgc ggctcgtccg ccatcaggcc
     2221 tgccggacgc ggggaggctg catctccggc acggtgcggt ccaggtaatc gcgggcctgc
     2281 tggtccatga tgctgcgcat cacgcggcgg aagccgtcca ccgcgatggg gccggcttcc
     2341 cggtaggcga gggtcaggcg ttcccgctgg cgttcgaagg cgcggcgttc cagtgcgtgg
     2401 cccttcgcgg tcagggtcag cagccgctgg cgccggtcct cgcggccggg ggtctgggcc
     2461 acatagcctt gctccaccag caggctcagc acgcgggcca gcgattgttt ggtgatgcgc
     2521 aaaatcacca gcaggtcgga caccgtgatg ccgggattgc ggccgatgta gtgcagcgcc
     2581 cgatgatgcg cgcgccccag tccgagttct tccagaatca cgtctgcggc attggtgaag
     2641 tcgcggtagg cgaagaacaa taaatcctgg gcaagacgca tatctgcctc tgcggggaac
     2701 aggctgccgg atgccggggc cggtggcgcg ccgggttcca gcaggctgag accggcgcgg
     2761 ctgcggcggg gatcgacagc gcccttcggt tcggg
//
LOCUS       DASSZH010000410         3081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_708643, whole genome shotgun sequence.
ACCESSION   DASSZH010000410 DASSZH010000000
VERSION     DASSZH010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708643"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFN42_08890"
     CDS             <1..297
                     /locus_tag="VFN42_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HET8996770.1"
                     /translation="ATLVFRHHAVAGPAAGEVSGHYHPKATVAARGGSVSRPCFIGDS
                     RRLMLPAFGAYTGGLDIRDASIARFFPRGARVFLLGKDRLFSFSFGSLRQGQVA"
     gene            665..1450
                     /locus_tag="VFN42_08895"
     CDS             665..1450
                     /locus_tag="VFN42_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996771.1"
                     /translation="MNTVKMSQRQAAFRDDFRRRIAPAYSGWTHVALIYALGLAAIWF
                     CARQIVHPAWYEFLVIPVAFCISNVFEWWIHRYVMHRPVKGFMGIYKRHTLAHHQFFT
                     HREPTFDNTRDFRIVFFPPYALVAFMALSIPPAAILGALGLPNAGWLLLITNVALYLN
                     YELFHYCCHVKDDRLVRHIPLVNSIRRHHIAHHNTALMMERNFNLTYPVADWFFGTSD
                     LACGLLKHVFNGYDQSRVRRDLKRVRNSSDDVHAGRVQQAAAE"
     gene            1447..1632
                     /locus_tag="VFN42_08900"
     CDS             1447..1632
                     /locus_tag="VFN42_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996772.1"
                     /translation="MSTQRIKPGKASLIGGIAVAFAALVLWTAIAHDLAADSVPVLIA
                     GVLVALGVGVWIRVADL"
     gene            complement(1869..>3081)
                     /locus_tag="VFN42_08905"
     CDS             complement(1869..>3081)
                     /locus_tag="VFN42_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996773.1"
                     /translation="QLFVHAIGAAGHDVVKYALDTYASADGASLSCTHDANAWPSDRY
                     AGLPAPRDDERVVLWLQNSHAVPIPAGSVALDRMGAERPVMLAREIGPFASLALDVAQ
                     LLPDLRWPAQIELRSGRHMVRPRYEVTRGGRTRIAHVNVERADLRPDPGIPALPPQLG
                     RGYLLPFPVLPRARFRSIVQPTPMAETQTSLPVRLDVFDADGRCVAQRFLGCLSRDHA
                     VAVDFDAIDVAGGHAELVYDFRDGGSADGWLHALFRYEDRESGHAAESSFGGHIFNTL
                     MTYKDEPQSYSGPPPGLSTRLFLKLGDARRRSFAVLIYPASAAWHPRSSTTLILHDSS
                     GQVVAEKPLSIACSGSAMVWPHAVFGADLLQHAGADGYVLVRDTTCRLFGYHGLMDDS
                     GGFSLDHMFGF"
BASE COUNT          516 a         1050 c          985 g          530 t
ORIGIN      
        1 gccacgctgg tgttccgtca tcatgccgtc gccggtcctg cggccggcga ggtcagcgga
       61 cattatcatc cgaaggcgac ggtggccgcg cgcggcggat cggtgtcacg cccctgcttc
      121 atcggcgact cccggcggct gatgctgcct gcctttggtg cgtacaccgg cgggctggac
      181 attcgcgatg catcgatcgc gcggttcttc ccgcgtggcg cgcgtgtctt cctgttgggc
      241 aaggatcggc tattcagctt cagctttggc agtctgcgcc agggccaggt ggcctgaacg
      301 cgccgcttcg ctcgggactt gcggcggaac ttgcttgaca aacaccgtga aaagtgcctg
      361 gatattcgca tcgcaacaag cggaggaggg tgcaccacag catgaccact ccacctgggt
      421 tgccaggggt cgtcgacctg cttggcatcg gcggcactcc cttgaagtcc aagtcatatc
      481 ccggccctta gcgccgagag cgatggctgt atgcctcgca accgaccccc gtttgtcgca
      541 gcatgcgcac gggggtcttt gcgttctggc gcatccccgc aacacgccgg cttgaccgcg
      601 gcggcacagt tgcctgccgc cagcggttct gttttgctgt ccacccgctt tgggaggact
      661 gtcaatgaat acagtgaaaa tgtcgcagcg gcaggcggcg tttcgcgatg atttccgccg
      721 ccgcatcgcc ccggcctatt ccggctggac gcacgttgcg ctaatctacg cgctcggtct
      781 tgccgcaatc tggttctgcg cccggcagat cgtgcatccc gcctggtacg agttcctggt
      841 gatcccggtc gccttctgta tctccaatgt gttcgagtgg tggattcacc gctacgtgat
      901 gcaccgcccg gtcaagggct tcatgggcat ctacaagcgc cacaccctgg cgcatcacca
      961 gttcttcacc catcgcgaac ccaccttcga caacacgcgc gacttccgca tcgtgttttt
     1021 cccgccctat gcgctggtcg ccttcatggc gctgtcgatc ccgccggcgg caatcctggg
     1081 cgcgctgggc ctgccgaatg ccggctggct gctgctgatc accaacgtcg cgctgtacct
     1141 gaactacgag ctgttccact attgctgcca tgtgaaggat gaccggctgg tgcggcacat
     1201 cccgctggtg aactccatcc gccggcatca catcgcgcac cacaacaccg cgctgatgat
     1261 ggaacgcaac ttcaacctga cctatccggt tgccgactgg ttcttcggca catcggatct
     1321 cgcctgcgga ttgctgaagc acgtgttcaa cggctacgac cagagccgcg tgcgccgcga
     1381 cctgaagcgc gtgcgcaaca gcagcgacga cgtgcacgcc ggccgcgtgc aacaggctgc
     1441 cgcggaatga gcacgcagcg gatcaagccc ggcaaggcga gcctgatcgg cggcatcgcg
     1501 gtcgcattcg ccgcattggt gctgtggacg gcgatcgcgc acgacctcgc ggcagacagc
     1561 gtgccggtgc tgattgccgg cgtgttggta gcgctcggcg tgggcgtctg gattcgcgtt
     1621 gcggatctgt agtcaggccc agctgctcgc cggccacgtc acaaccattc gcaatgacgg
     1681 gtcataccag cggcccaaag tcgtgaccgg tgctcggccc ttcaccgtca cggccggtga
     1741 gggcagtgag ggccggccat ccacgtctgg ctggcggtag ctcgcgcatg agacagaggt
     1801 gaatggccgg ctttcggcca tgactgtggc tgtgctcgcc gacgagccgg acgttcgctc
     1861 cccgggcatt agaacccgaa catatggtcc agcgagaacc cgccggaatc gtccatcagc
     1921 ccgtgatagc cgaacagccg gcaggtggta tcacgcacca gcacatagcc atcggcgccg
     1981 gcatgttgca gcagatcggc gccgaacacc gcatgcggcc agaccatcgc cgatcccgag
     2041 caggcgatcg acagcggctt ctccgccacc acctgcccgc tgctgtcgtg caggatcagc
     2101 gtggtgctgg agcgcggatg ccaggcggcg gacgcgggat agatcagcac cgcgaagctg
     2161 cgccgccgcg cgtcacccag cttcaggaac agccgcgtcg acaggcccgg cggcgggccg
     2221 gaataggatt gcggctcatc cttgtaggtc atcagcgtat tgaagatgtg gccgccgaaa
     2281 ctggactccg cggcatgacc ggactcccgg tcctcgtagc ggaacagcgc gtgcaaccag
     2341 ccatccgcgc tgccgccatc gcggaaatca tagaccagtt cagcgtggcc gcccgccacg
     2401 tcgatggcgt cgaaatccac cgccaccgca tggtcgcgtg acaggcagcc gaggaagcgt
     2461 tgcgccacac agcgcccgtc cgcgtcgaac acatccagcc gaaccggcag cgaggtctgc
     2521 gtctccgcca tcggcgtcgg ctgcacgatg ctgcggaaac gcgcacgtgg cagcaccggg
     2581 aagggcagca ggtagccgcg gccaagctgc ggcggcagcg ccggaatgcc cggatctggc
     2641 cgcagatccg cgcgctccac gttgacatgc gcgatgcggg tgcggccgcc gcgggtgacc
     2701 tcgtagcggg ggcgcaccat gtggcggccg gaacgcagct cgatctgcgc cggccagcgc
     2761 agatccggca gcagctgcgc cacatccagc gcgaggctgg caaacggccc gatctcccgg
     2821 gccagcatca ccgggcgttc cgcgcccatc cggtcgagcg cgacgctgcc cgccgggatc
     2881 ggcacggcgt ggctgttctg caaccacagc accacccgct cgtcatcgcg cggcgccggc
     2941 aaaccggcat agcgatcgga cggccaggca ttggcgtcat gcgtgcacga caggctggcg
     3001 ccatctgccg atgcgtaagt gtccagcgcg tacttcacca cgtcatgccc ggcggcgccg
     3061 atcgcgtgca cgaacaactg g
//
LOCUS       DASSZH010000411         2138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1066707, whole genome shotgun sequence.
ACCESSION   DASSZH010000411 DASSZH010000000
VERSION     DASSZH010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066707"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="VFN42_08910"
     CDS             complement(<1..1146)
                     /locus_tag="VFN42_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HET8996774.1"
                     /translation="MRYSLLTLLREAMRDQSGWTPAWRDAAPRDEYDTVIIGGGGHGL
                     ATAYYLASQHGITDVAVLEKGHIGSGNAGRNTTIIRSNYLLPGNEAFYEKSMQLWEGL
                     EQDLNYNAMVSQRGVLNLFHSDAQRDAFARRGNSMILHGADAELLDAEAVRRMVPQLD
                     FDNARFPIRGGLLQRRGGTARHDAVVWGYARAADQRGVDIVQNCEVTGLRIEHGRITG
                     VDTTRGFIRARRVGIAVAGNSSRVAAMAGMRLPIESHVLQAFVSEAVKPLIDTVVTFG
                     AGHFYISQSDKGGLVFGGDIDGYNSYAQRGSIPAMQDVMESAMALMPMLGRLRMLRQW
                     GGIMDMTMDGSPIIDRTPVEGLYLNAGWCYGGFKATPASGWCFAHTIA"
     gene            1207..>2138
                     /locus_tag="VFN42_08915"
     CDS             1207..>2138
                     /locus_tag="VFN42_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HET8996775.1"
                     /translation="MPRNDITRPDGDMRPETIGFLLIPGFALLPYACAVEPLRAANVL
                     SGRTLYAWRHFSPDGQPVAASNGVVIDAAALPDSASEVGALFVCAGGNPATFRHAPTF
                     ARLRRLSRQGMRIGGISGGPYLLARAGLLHGHRCTLHWEHAAAFAEEFPELDLRRSLF
                     EIDRDRLTSSGGTAALDMMHALIAQAHGPAIAAAVSDWFLQTEIRTGTAPQRMALRDR
                     LGVRSAPLLRALAAMEARLEQPCSRQELAAAAGVSTRQLERLFRAELGRKPGAHYLGL
                     RLERARQLARQTDLPLAEIAAACGFSSGSHFARS"
BASE COUNT          305 a          754 c          739 g          340 t
ORIGIN      
        1 ggcgatggta tgggcgaagc accagccgga ggccggcgtg gccttgaacc cgccatagca
       61 ccagccggca ttcagataga ggccctcgac cggcgtgcgg tcgatgatcg ggctgccatc
      121 catggtcatg tccatgatgc cgccccattg gcgcagcatg cgcaggcggc cgagcatcgg
      181 catcagcgcc atggcgcttt ccatcacatc ctgcatcgcc ggaatgctgc cgcgctgggc
      241 ataggagttg tagccgtcga tatcgccgcc gaacaccagc ccgcccttgt ccgactggct
      301 gatgtagaaa tgcccggcgc cgaaggtcac caccgtgtcg atcagcggct tcaccgcttc
      361 cgacacgaag gcctgcagca cgtggctttc gatcggcagc cgcatgccgg ccatggccgc
      421 gacgcgcgag gaattccccg ccaccgcgat gcccacccgc ctggcgcgga tgaagccgcg
      481 cgtggtgtcc accccggtga tgcggccgtg ttcgatgcgc aggccggtga cctcacagtt
      541 ctgcacgatg tccacgccgc gttgatccgc cgcgcgggca tagccccaca ccaccgcatc
      601 gtgccgcgcg gtgccgccgc ggcgctgcag caggccgccg cggatcggga agcgggcgtt
      661 gtcgaagtcc agctgcggca ccatgcggcg caccgcttcg gcatccagca attcggcatc
      721 ggcgccatgc aggatcatgc tgttgccgcg ccgcgcgaag gcgtcgcgct gcgcatcgct
      781 gtggaacagg ttcagcacgc cgcgctggct gaccatggcg ttgtagttga ggtcctgctc
      841 caacccctcc cacaactgca tcgacttttc gtagaaggcc tcgttgcccg gcagcaggta
      901 gttggaacgg atgattgtgg tgttccgccc ggcattgccg gagccgatat ggcccttctc
      961 cagcaccgcg acatcggtga tgccgtgctg gctcgccagg taataggcgg tggccagccc
     1021 atgcccgccg ccgccgatga tcaccgtgtc gtattcgtcg cgcggcgccg cgtcgcgcca
     1081 ggcaggcgtc cagccgctct ggtcgcgcat cgcttcacgc agcagcgtca gcagggagta
     1141 tcgcatcggt cctcgtgtgc ccggccgctt gacccgtcct gcttaagccg ggagggtggg
     1201 cggcacatgc ccaggaacga cattaccagg cccgacggcg acatgcggcc ggagacgatc
     1261 gggttcctgc tgatccccgg cttcgcgctg ctgccctatg cctgcgcggt ggagccgctg
     1321 cgggcggcca atgtgctgtc cggacggacg ctgtacgcct ggcggcattt ctcgccggac
     1381 ggccagccgg tggcggcctc caacggcgtg gtgatcgacg cggcggcgct gccggattcc
     1441 gcatcggagg tcggcgcgct gttcgtctgt gccggcggca atcccgccac cttccgccac
     1501 gcgccgacct ttgcccggct gcgccggctc agccggcagg gcatgcggat cggcggcatc
     1561 tccggcggtc cctacctgct ggcacgcgcc ggcctgctgc acgggcaccg ctgcacgctg
     1621 cactgggagc atgcggcggc cttcgcggaa gagtttccgg agctcgacct gcggcgctcg
     1681 ctgttcgaga tcgaccgcga ccggctgacc agcagcggcg gcaccgcggc gctggacatg
     1741 atgcatgcgc tgattgcaca ggcccatggt ccggccatcg ccgccgcggt gagcgactgg
     1801 ttcctgcaga ccgagatccg caccggcacc gcgccgcagc ggatggcgtt gcgcgaccgg
     1861 ctgggcgtgc gcagcgcgcc gctgctgcgc gcattggcgg cgatggaggc gcgactggag
     1921 cagccctgca gccggcagga actggccgcc gccgccgggg tctccacgcg gcagttggag
     1981 cggctgttcc gcgccgaact ggggcgcaag ccgggcgcgc actacctggg gctgcgcctg
     2041 gagcgggcgc ggcaattggc gcggcagaca gacctgccgc tggcggaaat cgccgcggcc
     2101 tgcggcttca gcagcggcag tcatttcgcc cgcagcta
//
LOCUS       DASSZH010000412         3175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1083434, whole genome shotgun sequence.
ACCESSION   DASSZH010000412 DASSZH010000000
VERSION     DASSZH010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083434"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1032
                     /gene="era"
                     /locus_tag="VFN42_08920"
     CDS             136..1032
                     /gene="era"
                     /locus_tag="VFN42_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET8996776.1"
                     /translation="MTQSRCGFVAIVGAPNAGKSTLLNRLTGARLSIVTPKAQTTRFR
                     VLGILMHGESQVLLVDTPGIFRPRRRLDRAMVAAAWTGAQDADLALLLVDSRAGVTDE
                     VRGIAGRLAEGKRRAWLVLNKTDLVERPRLLPLAATLSELAPFERTFMVSATTGDGIA
                     DLAAALAEAMPEGPHLYPDDDLTDLPDRLLAAETVREQIFLQTHEEVPYATTVETENW
                     QERKDGSVRIEATIYVLRAAHKAILIGERGARIKQIGARARQELEQVLERKVHLFLNV
                     KQRAGWDEETARLRAIGLEDKG"
     gene            complement(1046..1936)
                     /gene="ltnD"
                     /locus_tag="VFN42_08925"
     CDS             complement(1046..1936)
                     /gene="ltnD"
                     /locus_tag="VFN42_08925"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HET8996777.1"
                     /translation="MKVGVIGLGTMGMGAALNLHRKGHAVTGCELREPVRAEFAAAGG
                     ATVTSADQLPTDTEAVLVFVINAAQTEEVLFGQHGCLAKLNKGAVILCCATVPPDAAR
                     GLGRRITEAGFLMLDSPVSGGKAGAQAGTMTVMGSGSDAAFAKAKPVLDAISTKVWQL
                     GAEPGIGSTVKMVNQLLAGVHIATAAEALSLGIRAGADPKVLYEVISSSAGSSWMWQN
                     RVPHILEGDDTPLSAVNIFVKDLGIVLDQARSLTFPLPLASAAHQLFLAAAAHGEGMK
                     DDSFVIRVWQALTGIELPKK"
     gene            complement(2015..>3175)
                     /locus_tag="VFN42_08930"
     CDS             complement(2015..>3175)
                     /locus_tag="VFN42_08930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET8996778.1"
                     /translation="EALGGAWFDMGAVWLHSAEHNPLVPIAQAAGDTLLRSDELRRER
                     TMIGAREATPAEYADYEQAWRRFEATADRLLREFPDTSLAQVARHMPGDPWALTVESF
                     EGPVICAVAADKFSLRDWRDNVLSGSNLVPQGGIGAFVQRRLGQGLDIRLNTPVSRVR
                     WNRPGGRVAVETTRGTITAAACIVTVSTGVLGAGALAFDPALPAGTRDSIAALPMGLA
                     VKVALRATGTDRLDLPLHCSLDHQVQRSGEPFMVFQCWPYGRDYVQGWIGGDPAWDLA
                     RAGEAAAADFALAELRRMFGGRVDRLFAGGGRLVTHWDADPWIRGAYCYAEVGAADAR
                     SRLAEPLGGGHLLIAGEACHDGLGGTLAGAWISGQRAAEHAAEALRRGGTGC"
BASE COUNT          483 a         1156 c         1078 g          458 t
ORIGIN      
        1 cgtggatctc aaggacctcc tcctcccctt gcgggagggg gttgggggga gggggccggc
       61 aagagttcac cgggcaacct cggacgcccc ctcccccccc ccccctcccg caaggggagg
      121 gggagtccaa cgcccatgac ccaatcccgc tgcggcttcg tggccattgt cggtgcgccg
      181 aatgccggca aatccaccct gctcaaccgg ctgaccggcg cccggctgtc gatcgttacc
      241 cccaaggcgc agaccacccg gttccgcgtg ctcggcatcc tgatgcacgg ggaaagccag
      301 gtgctgctgg tggatacgcc cggcatcttc cgtccccgcc gccggctgga tcgggcgatg
      361 gtggcggcgg cctggaccgg cgcccaggat gccgacctgg cactgctcct ggtcgattcc
      421 cgcgccgggg tgacggacga ggtgcgcggc attgccggac gcctggccga gggcaagcgg
      481 cgcgcctggc tggtgctgaa caagaccgac ctggtggaac gccccaggct gctgccgctc
      541 gccgccacgc tgtcggagtt ggcaccattc gaacgcacct tcatggtcag cgcgacgact
      601 ggcgacggca ttgccgacct ggcggcggcc ctggcggaag cgatgccgga ggggccgcat
      661 ctctatccgg acgacgacct gaccgacctg cccgaccggt tgctggcagc ggagaccgtg
      721 cgcgaacaga tcttcctgca gacgcatgag gaagtgccct acgccaccac ggtggaaacc
      781 gagaactggc aggagcgcaa ggacggctcg gtgcgcatcg aggcgacgat ctatgtgctg
      841 cgtgcagcgc ataaggcgat cctgatcggc gagcgggggg cccgcatcaa gcagatcggt
      901 gcccgcgcgc ggcaggagct ggagcaggtg ctcgaacgca aggtgcatct gttcctgaac
      961 gtcaagcagc gcgccggatg ggacgaggag accgcgcggc tgcgcgcgat cgggctggag
     1021 gacaaggggt agtccggcca gcgcgctact tcttcggcag ctcgatcccg gtcagcgcct
     1081 gccacacacg gatgacgaag gaatcgtctt tcatcccctc gccatgcgcc gcggcagcca
     1141 ggaagagctg atgcgccgcc gaggccagcg gcagcggaaa ggtcagcgac cgcgcctggt
     1201 ccagcacaat gcccaggtcc ttgacgaaga tgttcaccgc ggacagcggc gtatcgtcgc
     1261 cctccaggat gtgcggcacg cggttctgcc acatccagga actgccggcc gagctggaga
     1321 tcacctcgta cagcactttc ggatcggcgc cggcgcggat gcccagggac agcgcctcgg
     1381 ccgcggtggc gatgtgtacg cccgccagca actgattcac catcttcacc gtgctgccga
     1441 tccccggctc cgcgcccaat tgccacacct tggtggagat cgcgtccagc accggcttcg
     1501 ccttggcaaa ggcggcgtcg gagccggatc ccatcaccgt catggtgccg gcctgcgcgc
     1561 ccgccttgcc gccggagacc ggcgaatcca gcatcaggaa gccggcctcg gtgatgcgcc
     1621 tgcccaggcc gcgcgcagcg tccggcggca cggtggcgca gcacaggatc accgcgccct
     1681 tgttcagctt tgccagacag ccatgctggc cgaacagcac ctcctcggtc tgcgcggcgt
     1741 tgatcacgaa caccagcacg gcttcggtat cggtgggcag ttgatcggcg gaggtgaccg
     1801 tcgcgcctcc ggccgcggcg aattccgcgc gcaccggctc gcgcagttcg cagcccgtca
     1861 cggcgtgtcc cttgcggtgc aggtttagtg ccgcacccat tcccatggtg ccgaggccga
     1921 tcacgccgac tttcatgctc gtatccctgt cacagcggcg gccccggtca tggaagccgc
     1981 ttccgacggg gagttttggt cctcgccctg cggttcagca accggtcccg ccgcgccgga
     2041 gagcctccgc cgcgtgttcc gccgcgcgct ggccgctgat ccaggcgccg gccagcgtgc
     2101 cgcccaggcc gtcgtggcac gcctcgccgg cgatcaacaa atggccgccg cccagtggct
     2161 cggccagccg ggaccgcgca tcggcggcgc ccacctcggc atagcaatag gcgccacgga
     2221 tccaggggtc ggcatcccag tgcgtcacca gccgcccgcc gccggcgaac agccggtcca
     2281 cccggccgcc gaacatccgg cgcagttcgg ccagagcgaa gtcggcggcg gccgcttctc
     2341 cggcgcgggc gaggtcccag gccggatcgc cgccgatcca gccctgcacg tagtcccggc
     2401 catagggcca gcactggaac accatgaagg gctcgccgct gcgttgcact tggtggtcca
     2461 gcgagcaatg cagcggcagg tccagccggt ccgtgccggt ggcgcgcaag gccactttca
     2521 ccgccagccc catgggcagt gcggcgatgc tgtcccgcgt accggccggc aaggccgggt
     2581 cgaacgccag ggcgccggcc cccagcaccc cggtggagac ggtgacgatg caggccgcgg
     2641 cggtgatcgt accgcgagtg gtttcgacgg caacccgtcc gcccggcctg ttccagcgca
     2701 cccggctcac cggggtgttc agcctgatat ccagcccctg ccccagccgc cgctgcacga
     2761 aggcgccgat gccgccctgc ggcaccaggt tgctgccgga cagcacgttg tcgcgccaat
     2821 cgcgcaggct gaacttgtcc gccgccacgg cgcagatcac cggtccctcg aagctctcca
     2881 ccgtcagcgc ccagggatcg cccggcatgt gccgcgccac ctgcgccagc gaggtgtcgg
     2941 gaaattcccg cagcagccgg tcggcggtgg cctcgaagcg gcgccaggcc tgctcgtagt
     3001 cggcgtattc cgccggcgtg gcctcccgcg caccgatcat ggtgcgctcg cgccgcagtt
     3061 cgtcggagcg cagcagcgtg tcgccggcgg cctgggcgat cggcaccagc gggttgtgtt
     3121 ccgcgctgtg cagccacacc gcgcccatgt cgaaccaggc gccgccgagc gcttc
//
LOCUS       DASSZH010000413         2382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_265693, whole genome shotgun sequence.
ACCESSION   DASSZH010000413 DASSZH010000000
VERSION     DASSZH010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265693"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..808
                     /locus_tag="VFN42_08935"
     CDS             155..808
                     /locus_tag="VFN42_08935"
                     /inference="COORDINATES: protein motif:HMM:NF017277.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HET8996779.1"
                     /translation="MRVDPFDRDDLKHGLGKLANGTARRFWRLCLYAPLCFDGRPARS
                     DPGLSMAAPKQSRIARKSFNCRQKSVIRLPVGEHDTVMNTPDEKLLAYAAQIVSAHVA
                     HNLVSTTALPRLIQDVVQSLRTAQLHGIAPERPKPAVPIQQSIQRDSIACLECGKRFR
                     TLKRHLEREHNMTDEEYRLRWQLPASYKLTAPEYAELRADVARKIGLGRKPRGRGKR"
     gene            945..1391
                     /locus_tag="VFN42_08940"
     CDS             945..1391
                     /locus_tag="VFN42_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996780.1"
                     /translation="MPSLPARAALGFIAAALSVLTFHQGVVELYHLAGMAGPAWSMRP
                     IPPFGVPLLIDLGFWGGLYGLVFGIVVPVLRPVWFWGIVLGCLAAAVGWVLVAPLKGL
                     PMGYGWHLRAMIRSLAINGSWGLGVGLILPLLMPSVPRRRRRFARV"
     gene            complement(1396..2295)
                     /locus_tag="VFN42_08945"
     CDS             complement(1396..2295)
                     /locus_tag="VFN42_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006116213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HET8996781.1"
                     /translation="MSDPGTPQRPARPGPKVSPIRRLRRLRARPRSRPRFKGPSFNRV
                     IPNLLTMLGLCAGLTALRFALEARFGAAAVAIAVAGAIDGLDGRLARLLKATTRFGAE
                     FDSLADFVCFGVAPAFTIYIWSLQRAGGFGYTPCLMFAVCMALRLARFNAALEGAPKP
                     HYAYNFFTGVPAPAGAGLALFPLFLGLEAKSLGWTWLLELAQYPLFTALVLAGSAVLL
                     VSTLPVWSFKNFKVPAEYVLPLLLGTGIFAAVLVADPWAALAAAGLIYLGMLPFSVRS
                     FHRLRREAEMPDDEADEQLRDAV"
BASE COUNT          396 a          805 c          811 g          370 t
ORIGIN      
        1 ctcgcgcagg cgcacgacca cctcccgccc gagttgaccg aattccggac ctcggtggtt
       61 gatcgcgttg cggtccatgg cacgcaatac gcgatcgggc acattggttg gtcccggcgt
      121 ttgcaggaag tgccggccgg cgacgaagct ggtcatgcgc gtggatccct tcgatcggga
      181 cgacctaaag cacgggctgg gcaagcttgc caacggcacg gcacgccgct tctggagact
      241 ttgcctttac gccccgctgt gcttcgacgg ccggccggcg agaagcgatc cgggcctttc
      301 gatggcggcg ccaaaacagt cacgaatcgc aagaaaatcg tttaactgcc gtcaaaagtc
      361 cgttatacga ctccctgttg gggagcacga cacagttatg aacactccgg atgaaaaact
      421 ccttgcctac gcggcacaga tcgtcagcgc ccatgtcgcc cacaatctgg tgtccaccac
      481 ggcgctgccg cggttgatcc aggatgtggt acagtcgctg cgcaccgcgc aactacacgg
      541 aatcgcgccc gaacggccca agcctgcggt accgatccag cagtcgatcc agcgcgattc
      601 gatcgcctgt ctggaatgcg gcaagcggtt ccgcacgctg aagcgccacc tggagcgcga
      661 gcacaacatg acagacgagg agtatcgcct gcgctggcag ttaccggcga gttacaagct
      721 gaccgcgccg gaatacgccg agctgcgcgc cgacgtggca cgcaagatcg gcctggggcg
      781 gaagccacgc ggccgcggca agcgctagtc ccgcgccccg cgcggcaggt gccgggcagc
      841 cgaggcgtgt cgtgccgcaa tgccgatcgc ggcacgacgt tgccgtgatt gacgcgcgcc
      901 cgcgcccgcg ctgaagctgc ggccagtggc aggggagaac cgccatgccg tcactgcccg
      961 cccgcgcggc gctgggcttc atcgcggccg cactgtcggt actgacattt catcaggggg
     1021 tggtcgaact ttaccatctc gccggcatgg ccggtcccgc ctggtcgatg cggccgatcc
     1081 cgccgttcgg ggtgcctttg ctgatcgacc ttggcttctg gggcgggttg tatggcctgg
     1141 tgttcggcat cgttgtgccg gtgctgcggc cggtctggtt ctggggcatc gttcttggtt
     1201 gcctcgccgc cgcggtcggc tgggtgctcg tggcaccgct gaaggggctg ccgatgggtt
     1261 atggctggca cctgcgggcg atgatccgtt cgctggcgat caatggcagt tggggcctgg
     1321 gggtcggact gatcctgccg ctgctgatgc cgtcggtgcc gcgccggcgc cgccggttcg
     1381 cccgggtcta gctgatcaga cggcgtcccg cagttgctcg tccgcctcgt cgtccggcat
     1441 ttccgcctcc cgccgcagcc gatggaaact gcggacgctg aagggcagca tgcccaggta
     1501 gatcaggccc gccgcggcca gtgccgccca gggatcggcg accagcacgg cggcgaaaat
     1561 tccggtgccc agcagcagcg gtagcacgta ttccgccggc actttgaagt tcttgaaact
     1621 ccacaccggc agcgtggaga ccagcagcac cgccgaaccg gccagcacca gcgcggtgaa
     1681 cagcgggtac tgtgccagtt ccagcagcca ggtccagccg agcgacttgg cctccaggcc
     1741 gagaaacagc gggaacagcg caaggccggc cccggccggc gcgggaacgc cggtgaagaa
     1801 gttgtaggca tagtgcggct tcggcgcgcc ctccagcgcc gcgttgaacc gcgccagccg
     1861 cagcgccatg cagacggcga acatcaggca gggcgtgtag ccgaacccgc cagcccgctg
     1921 cagcgaccag atatagatcg tgaaggccgg cgccacgccg aagcagacga aatcggccag
     1981 gctgtcgaac tccgcaccga agcgcgtggt ggccttcagc agccgcgcca gcctgccgtc
     2041 cagcccgtcg atcgcgccgg ccaccgcgat cgccaccgcg gcggcgccga accgcgcttc
     2101 cagtgcgaag cgcagcgcgg tcagcccggc gcacaggccc agcatggtca gcaggttcgg
     2161 gatcacccgg ttgaaggacg gtcccttgaa gcgcggtcgc gagcgcggcc gggcgcgcaa
     2221 ccggcgcaac cggcggatgg gagaaacctt ggggcccgga cgggccgggc gctggggcgt
     2281 gccgggatcg ctcatggcag gcgttccgct atcactgttt cgccgcccag catcgtctgg
     2341 ccttccacca ccacggggcg cagcccctgg ggcaggtaca ga
//
LOCUS       DASSZH010000414         3845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_725817, whole genome shotgun sequence.
ACCESSION   DASSZH010000414 DASSZH010000000
VERSION     DASSZH010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725817"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VFN42_08950"
     CDS             complement(<1..347)
                     /locus_tag="VFN42_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996782.1"
                     /translation="MEPQRFSCNSANMTGTIGDRMAAMAEAGFGSATLWPADLFVHFE
                     DPEATIALIEASPVAISAYQCVRDLEGSPPPVKARKMELARQFLDQMQLIGCDTLVLC
                     SNVNDELDRDWGRA"
     gene            529..1497
                     /locus_tag="VFN42_08955"
     CDS             529..1497
                     /locus_tag="VFN42_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET8996783.1"
                     /translation="MQRRRLGELEVGAIGLGCLTLTAAYGTPPPEADAIATIHRAAAL
                     GMDMLDTSDAYAKGDNERLIGRAIAGQRHRYVIASKFGNLRKPDGAPTANGRPEYVKQ
                     ACEASLRRLNTDVIDLYYIHRVDPAVPIEDTVGAMAALKREGKIRHLGISEAAPATIR
                     RAHAVHPMAAVQIEYSLWTRHVEAEVLPLCEALGIGFVGYSPLGRGFLAGAVATEPGT
                     GDIRRGMPRFSGDNLRHNLGLVASFARIAAAEGCSAAQLALAWVLSRRPPVVPIASSS
                     RRERLDENAAAASLTPSAQSLAAAERLFANVAGLRYGDAAARVVGL"
     gene            complement(1505..2206)
                     /locus_tag="VFN42_08960"
     CDS             complement(1505..2206)
                     /locus_tag="VFN42_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8996784.1"
                     /translation="MQRVALVTGAAHGIGAAVAARLARDGYRVVVADRDPGGAAPPGG
                     RYVVCDVADETAVRALLDGLRDQEQRLDALVCNAGIMIRKPLAQLSLAEWQQVLGTNL
                     TSSFLLVRAAETLLRAARGAVVTIASTRARMSEPDTEAYAASKGGLVGLTHALAISLG
                     PDVRVNCISPGWIDTKGDPLRPEDHAFHPAGRVGRTDDIAALAAFLLGPEAGFVTGAE
                     FVADGGVTRKMIYPE"
     gene            complement(2243..3556)
                     /locus_tag="VFN42_08965"
     CDS             complement(2243..3556)
                     /locus_tag="VFN42_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HET8996785.1"
                     /translation="MSKHPETLALHAGWRRDSATNAVAVPIYQTTSYQFDSAEHAANL
                     FGLRELGNIYTRIMNPTADAFEQRVAALEGGVAALAVASGQAASAFCVQNLARAGDNF
                     VASTDLYGGTWNLFANTLKDQGIEVRFVDPADPEAFARATDDRTRAYYAETLPNPKLI
                     PFPIAEVAAIGRKFGIPLIMDNTAAPTLVRPFDHGAAVVMYSATKYIGGHGTSIGGVV
                     IDGGNFDWEAHAARQPLLNTPDASYHGAIWAQAAKPLGPIAYILRMRVVLLRDLGAAL
                     SPFNAFLFLQGLETMPLRMREHSRNALAVANFLAQRKDVARVIHPAHHTGIGAERSAK
                     YMPHGQGGLVGFELAGGSDAGRRFIDALKMFYHVANIGDARSLAIHPASTTHSQLSEA
                     EQAATGVTPGYVRLSIGIEHIDDILADLTQALDAAGGSVTRKAAE"
BASE COUNT          621 a         1292 c         1314 g          618 t
ORIGIN      
        1 gcgcgacccc agtcgcgatc gagctcgtca ttgacgttgg agcacagcac cagcgtgtcg
       61 cagccgatca attgcatctg gtcgaggaat tgccgtgcca gttccatttt gcgcgccttc
      121 acgggtggcg gcgagccttc caggtcgcgc acgcattgat acgccgatat cgcaaccggg
      181 ctggcctcga tcagcgcgat cgttgcctcg gggtcttcga aatgcacgaa cagatcggcg
      241 ggccacagcg tcgcggaacc aaacccggct tccgccatgg cggccatgcg gtcgccgatc
      301 gtgccggtca tgttggccga attgcaggaa aaccgctgcg gctccatggc gttccccttg
      361 gcttcaagcg tccctggaaa aatgaaacca cagatgaatg cggatgaaca cagatggtgc
      421 gggcgcgcga agcatccggg ttgatccatt gcggcctttg gactgccgcg ccaacatcgg
      481 gtcggcgcga ccttacggcg agcaagaaca acaggcaggg aggcatggat gcaacgccgg
      541 cggctaggtg agctggaagt tggggccatc ggcctgggct gcctgacgct cacggcggcc
      601 tatggtacgc ccccgcccga agccgacgcg atcgcgacga tccaccgcgc ggcggcgctg
      661 ggcatggaca tgctcgatac gtcggatgcc tacgccaagg gcgacaacga gagactgatc
      721 ggccgggcga tcgccggaca gcgccatcgc tacgtgatcg ccagcaagtt cggcaatctg
      781 cgcaagcccg acggcgcgcc caccgccaac ggccggcccg aatacgtcaa gcaggcctgc
      841 gaagcgagcc tgcgccgact gaacaccgat gtgatcgacc tctactacat ccatcgcgtc
      901 gatccggccg tgccgatcga ggacacggtc ggcgccatgg cggcgctgaa gcgcgagggg
      961 aaaatccgcc acctggggat ttccgaggcc gccccggcga cgatccggcg ggcgcatgcg
     1021 gtgcatccga tggccgcggt gcagatcgaa tactcgctgt ggacccgcca tgtcgaagcc
     1081 gaggtcttgc cgctgtgcga ggcgcttggc atcggcttcg tcggctacag tccgctggga
     1141 cgtggcttcc tggccggcgc ggtcgcgacg gaacccggga caggcgacat acggcgcggc
     1201 atgccgcgat tttccggcga caacctacgg cacaatctgg gcctggtggc gtcgtttgcg
     1261 cgcattgccg ccgccgaagg ctgcagcgcg gcgcagcttg cgctggcctg ggtgctgtcg
     1321 cgacggccgc cggtggtgcc gatcgccagc agcagccggc gcgaacggct ggacgagaac
     1381 gccgccgccg cgtcgctgac gccgtccgcg cagagcctgg cggcggcaga gcgcctgttc
     1441 gcgaacgtcg ccgggctgcg ctacggcgat gcggcggcgc gggtggtcgg actgtagtgc
     1501 ggcatcactc gggatagatc atcttccgcg tcacgccgcc atcggccacg aattcggcgc
     1561 cggtgacgaa gcctgcctcc ggacccagca ggaacgcagc cagcgcggcg atatcgtccg
     1621 tcctgccaac ccgtccggcg ggatggaacg cgtggtcctc cggccgcaac ggatcgccct
     1681 tggtatcgat ccagccggga ctgatgcaat tcacccgcac atccggcccc aggctgatcg
     1741 ccagcgcatg cgtcagtccc accagcccgc ctttcgatgc cgcataggct tcggtgtccg
     1801 gctccgacat gcgcgcccgg gtggaggcga tggtcaccac tgccccgcgt gccgcgcgca
     1861 gcaatgtttc ggctgcgcgc accagcagaa agctgctggt gagattggtg ccgagcacct
     1921 gctgccattc cgcgagggag agttgcgcga gcggcttgcg gatcatgatg ccggcattgc
     1981 acaccagcgc gtccagccgc tgctcctggt cgcgcaggcc atccagcagc gcgcgcacgg
     2041 cggtctcgtc cgccacgtcg cagacgacgt agcggccacc cggcggggcg gcgccacccg
     2101 gatcgcggtc ggcgacgacc acgcgatagc cgtcgcgggc cagccgcgcg gccaccgcgg
     2161 cgccgatgcc gtgcgccgcg ccagtcacca gtgctacgcg ttgcatgccg tgcgccgcgg
     2221 ctgttcgcct gccacggtgc ggttactccg cggccttccg tgtcacgctg ccgccggccg
     2281 catccagcgc ctgggtcaag tcggccagga tatcgtcgat atgctcgatg ccaatcgaca
     2341 gccgcacata gcccggcgtc acgccggtgg ctgcctgttc cgcctccgat aattgcgagt
     2401 gcgtggtgct ggctgggtgg atcgccaggc tgcgcgcatc gccgatattc gccacgtggt
     2461 agaacatctt cagcgcgtcg atgaagcgcc gcccggcatc gctgccgcca gccagttcga
     2521 acccgaccag gccgccctgg ccatgcggca tgtatttggc gctgcgttcc gcgccaatgc
     2581 cggtgtgatg cgccgggtgg atcacccgcg ccacatcctt gcgctgcgcc aggaagtttg
     2641 ccaccgccag cgcgttccgg ctgtgctcgc gcatgcgcag cggcatggtt tctagcccct
     2701 gcaggaacag gaaggcattg aacggcgaca gcgcggcacc aagatcgcgc agcagcacca
     2761 cgcgcatgcg gaggatatag gcgatcgggc ccagcggctt ggcggcctgc gcccagatcg
     2821 cgccatgata gctcgcgtcc ggcgtgttca gcagcggctg gcgcgcggca tgcgcctccc
     2881 agtcgaaatt gccgccgtcg atcaccaccc cgccgatcga ggtgccgtgg ccgccgatgt
     2941 atttcgtcgc cgagtacatc accaccgcgg cgccatgatc gaacggccgc accagcgtcg
     3001 gtgcggcggt gttgtccatg atcagcggaa tgccgaactt gcggccgatc gcggcgactt
     3061 cggcgatggg gaaggggatc agcttcgggt tcggcagtgt ctccgcgtag tatgcgcggg
     3121 tgcggtcgtc ggtggcgcgg gcgaacgctt ccggatcggc cgggtcgacg aaacgcactt
     3181 cgatgccctg gtccttcagc gtgttggcga acaggttcca ggtgccgccg tacagatcgg
     3241 tagaagcgac gaagttgtca ccggcgcgcg ccaggttctg cacgcagaac gccgatgccg
     3301 cctggccgga cgcgacagcc agtgccgcca cgccgccttc cagtgccgcg acgcgctgct
     3361 cgaacgcgtc ggcggtggga ttcatgatgc gcgtgtagat gttgcccagt tcgcgcagcc
     3421 cgaacaggtt ggcggcgtgc tccgcgctgt cgaactggta ggacgtggtc tggtagatcg
     3481 gtacggccac cgcattggtg gcactgtcgc gccgccagcc ggcatgcagc gccagggttt
     3541 ccggatgttt cgacatgggt gctctccccg ctcaggttgc gcgactatag cggcggcaac
     3601 gcgcgcgtga agcgcggcga ggaattcgca cccaatgctg gatcagggca gttgaatctc
     3661 tacgggaggc gggaaacgac aaaatcgcct cccgggcggg tcataccagg ctgcggccgg
     3721 ccaactgttc cagcacccgc accggtgcct gcccgggatg ctgcatggct tccaccggca
     3781 ggtggaaggt ctgttccagc gcgcaatggc cggcgatcgc ggcgtgcagc acctggtcgg
     3841 tgaag
//
LOCUS       DASSZH010000415         2942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1680560, whole genome shotgun sequence.
ACCESSION   DASSZH010000415 DASSZH010000000
VERSION     DASSZH010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680560"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="VFN42_08970"
     CDS             complement(<1..1023)
                     /locus_tag="VFN42_08970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HET8996786.1"
                     /translation="MTRPGGSAKAAPRWLAALPIGLAVFGRHSELIFANPRFLTVLGL
                     DAPQPKLSGLADLLETLRRVDAEGAALLASQFPPGQDTSDPVRWRRGDTVIDSSVTRL
                     PDGGQLCAVTARQETDPAPDAVIEARRAQQAAEAACQAKSHFLATVGHELRTPLNAVI
                     GFSEAMLREADNPSPARVAEFARQIGESGRTLLGLINVILDVASIESGRFALASAPID
                     VARLIRSAMRQVDAVAQAAELALATELPASLPPVHGDERRLLQVLHHLLSNAVKFTEA
                     GGTITVGARQEGGDTLLIFVRDSGIGIAPEELERAFQPFTHGGAASSGPSQGARLGLY
                     VSRALVT"
     gene            complement(1020..2105)
                     /locus_tag="VFN42_08975"
     CDS             complement(1020..2105)
                     /locus_tag="VFN42_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HET8996787.1"
                     /translation="MPFDVRDFSGAPPNDRAASLQNRRLVAIWLFSVAGMILVMVVLG
                     GVTRLTGSGLSIMEWEPFRGVLPPLNHAQWEKLFALYKQIPQYKLVNQGFGLAGFKHI
                     FWLEWTHRLWGRLIGLAFLLPLIWFWATGRLMREMRGWLVLLFCLGGLQGFVGWFMVS
                     SGFFPDSTHVAPARLVIHLSLALVLYAAIVWTGLTVLRPAPAYPSQSAPLRWLARACC
                     VSVALTIVAGGFVAGTHAGFTYNTFPLMDGSLIPRSYDRLSPFIRNLTQNLAAVQFDH
                     RLLATLTVLLATATVVTGFVRPRPDSVTGGLVALGVTVAVQYGLGVATLLLVVPVRLA
                     AAHQANAVLVLTAALVLLHAVRSPVRT"
     gene            2198..2713
                     /gene="lspA"
                     /locus_tag="VFN42_08980"
     CDS             2198..2713
                     /gene="lspA"
                     /locus_tag="VFN42_08980"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET8996788.1"
                     /translation="MKRNPLTRLGLVAGLLVLAADQASKAWMLYGLDLPRIRQIVLLP
                     VLNLTFVENRGVTFGMLNGFGGWSSVALAVVALAVVVALGIWLRRAETRIMAIAIGAI
                     AGGALGNVIDRLRLGWVVDFIDVHLGTWHWFVFNVADAAIVCGVAALVLESQWGRKAS
                     APRPQAEDNGA"
     gene            complement(2710..>2942)
                     /locus_tag="VFN42_08985"
     CDS             complement(2710..>2942)
                     /locus_tag="VFN42_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996789.1"
                     /translation="TTTNFVEHPEVVARRLEEAVAAVGDRERVIAGTDCGFGTFAGRE
                     YVAEEIVWVKLAAAAEGARIASRRLWGRAAGD"
BASE COUNT          475 a         1037 c         1025 g          405 t
ORIGIN      
        1 ggtcaccagg gcgcgggaga cgtacaggcc cagccgggcg ccctgcgacg ggccggacga
       61 cgcggcgccg ccatgggtga acggctgaaa cgcccgctcc agctcttccg gcgcaatgcc
      121 gatcccgcta tcgcgcacga agatcagcag cgtgtcgccg ccttcctgtc gcgcgccgac
      181 ggtgatcgtt ccgccggctt cggtgaattt caccgcgttc gacagcaggt ggtgcagcac
      241 ctgcaacagc cgccgctcgt caccgtgtac cggtggcagg ctcgccggca gctcggtcgc
      301 cagggcgagc tccgcagcct gcgccaccgc atccacctgg cgcatggcgc tgcggatcag
      361 ccgggcgacg tcgatcgggg cggacgccag tgcgaagcgg ccggattcga tgctggcaac
      421 gtccaggatg acgttgatca gccccagcag cgtgcgtccg gactcgccga tctgccgcgc
      481 gaactccgcg acgcgcgccg gcgagggatt gtccgcctcc cgcagcatcg cctcggagaa
      541 gccgatcacc gcgttcagcg gcgtgcggag ttcatggccc acggtggcca ggaagtgcga
      601 cttcgcctgg caggctgcct ccgccgcctg ctgcgcgcgc cgcgcctcga tcaccgcgtc
      661 gggggccggg tctgtctcct gccgcgctgt gacggcgcag agctggccgc cgtccggcag
      721 ccgggtcacg ctgctgtcga tcacggtatc gccgcgccgc cagcgcacag ggtcggatgt
      781 gtcctggcca ggcgggaact gggatgccag cagtgccgcg ccttcggcat cgacgcgccg
      841 cagcgtttcc agcaggtccg ccaagccgga gagcttcggc tgcggcgcat ccaggccgag
      901 caccgtcagg aagcgtggat tggcgaagat cagctcggag tgccgcccga acaccgccag
      961 tccaatcggc agcgccgcca gccagcgcgg cgccgctttc gccgaaccgc ctggccttgt
     1021 catgtccgca ccgggctgcg cacggcgtgc agcagcacca gggcggcggt cagcaccagc
     1081 accgcattgg cctgatgcgc cgccgccagc cggaccggga cgaccagcag cagggtcgcc
     1141 acacccagcc cgtattgcac cgccacggtc acccccagcg ccaccagccc gcccgtcacg
     1201 ctgtcgggcc ggggccgcac gaagccggtg accacggtgg cggtcgccag cagcacggtc
     1261 agtgtggcca gcagccggtg gtcgaactgc accgccgcga ggttctgcgt caggttgcgg
     1321 atgaacggcg acagccggtc ataggagcgc gggatcaagg agccgtccat cagcgggaag
     1381 gtgttgtagg tgaacccggc atgcgtgccg gccacgaacc cgccggcgac gatggtcagc
     1441 gcgacggaca cgcagcaggc ccgcgccagc cagcgcagcg gggcggattg cgaggggtag
     1501 gccggggccg gccgcagcac ggtcaggccg gtccacacga tcgccgcata cagcaccagg
     1561 gcgagcgaca ggtggatcac cagccgcgcc ggcgcgacat gcgtggaatc cgggaagaag
     1621 ccggacgaca ccatgaacca gccgacgaaa ccttgcagcc cacccaggca gaacagcagc
     1681 accagccagc cgcgcatctc ccgcatcagc cggccggtgg cccagaacca gatcagcggc
     1741 agcaggaagg ccaggccgat cagccgcccc cacagccggt gggtccattc cagccagaag
     1801 atgtgcttga acccggccag cccgaagccc tggttgacca gcttgtactg cgggatctgc
     1861 ttgtagaggg cgaacagctt ctcccactgg gcgtggttca atggcggcag cacgccgcgg
     1921 aacggttccc attccatgat cgacaggccg gagccggtca gccgcgtgac cccgcccagc
     1981 accaccatca ccaggatcat gcccgcaacg ctgaacagcc agatcgccac caggcgacgg
     2041 ttctgcaggc tggcggcccg gtcgttcggc ggggcgccgg aaaaatcacg cacatcgaag
     2101 ggcatgtgcg cgactatagg cagagcatga cggcaatgat actgcctctg cgcggtcgtc
     2161 cctccgctgc gtgcagggcc ggcccatctg gaggggcatg aagcggaatc cattgacgcg
     2221 tctgggcctg gttgccggcc tgctggtgct ggcggccgat caggcgagca aggcctggat
     2281 gctgtacggc ctggacctgc cgcggatccg gcagatcgtg ctgctgccgg tgctgaacct
     2341 gaccttcgtg gaaaaccgcg gggtgacgtt cggcatgctg aacgggttcg gcggctggag
     2401 cagcgtggcg ctggctgtgg tggcgctggc tgtggtggtg gcgctgggca tctggctgcg
     2461 ccgcgccgaa acccgcatca tggcgatcgc catcggcgcg atcgccggcg gtgcgctcgg
     2521 caatgtgatc gatcggctgc gcctgggctg ggtggtggac ttcatcgacg tccacctcgg
     2581 cacctggcac tggttcgtgt tcaacgtcgc cgatgcggcc atcgtctgcg gcgtggcggc
     2641 gctggtgctg gagagccaat ggggccgcaa ggccagcgct ccgcggcccc aggcggagga
     2701 taacggggcc tagtccccgg ccgcgcggcc ccatagccgg cgcgaggcga tgcgcgcccc
     2761 ctcggccgcc gccgccagct tcacccagac gatctcctcc gccacatatt cgcggccggc
     2821 gaaggtgcca aagccgcaat ccgtgccggc gatcacccgc tcccggtcac ccaccgcggc
     2881 gacggcttcc tccaaccggc gcgcgacgac ttccggatgc tcgacgaaat tggtggtggt
     2941 at
//
LOCUS       DASSZH010000416         3673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_691474, whole genome shotgun sequence.
ACCESSION   DASSZH010000416 DASSZH010000000
VERSION     DASSZH010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691474"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="VFN42_08990"
     CDS             complement(<1..847)
                     /locus_tag="VFN42_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012812747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8996790.1"
                     /translation="MKILEITNVDFSLRQFLLPLMRGARARGHDVIGVCADGRLLDAV
                     RAEGFRVITVPLQRRISPLAHLRALRHLVAVMRAERPDMVHAHMPISGFLARVAARIA
                     GVPRVAYTCHGFLFNQQGSWLHSSAGLLMECAAAPLTDIYLTVAESEAADARRLRFSR
                     TAMAVGNGRDPQRFRPDPEARARVRAALGTPETRPVVIAVSRLVRLKGYPELAAAMRA
                     VPDAELWVVGERLQSDRGGDMAALLANAGLGDRLRLLGYREDVAALLAAADIFVLPSH
                     FEGLPM"
     gene            957..1703
                     /locus_tag="VFN42_08995"
     CDS             957..1703
                     /locus_tag="VFN42_08995"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8996791.1"
                     /translation="MARLAGKIALITGGGTGIGRTTALLFAREGARVAVAEIDRAAGE
                     ATAHLAGNGAICIRTDVTDEASMRAAVRGTVQHFGGLHVLHNNAGGSTPQDNTVVEAP
                     VEEFWRVIKLDLFGTFLGCRFGIPAIIESGGGSVINMSSNVALMGIAGRDCYTAAKGG
                     VAAMTRSMAVEFAPQHVRVNAIAPSATMTERVRKLVAGNAALERMAQSHLLGLIEPED
                     VANLAVFLASDESRMVTGHIYPVDSGVTIS"
     gene            1714..2127
                     /gene="mce"
                     /locus_tag="VFN42_09000"
     CDS             1714..2127
                     /gene="mce"
                     /locus_tag="VFN42_09000"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HET8996792.1"
                     /translation="MKIKRVEHIAIAVDSLDRSAGLWRDAFGLDVEYEETIGETRLAM
                     LPVGESYLELLEGQGPESGVSQWIGEKGTGLFHICFEVEDIDGALAELKAKGVKLRDE
                     TPRIGHGGARIAFLDPASTGNVLVELAELPAAGGH"
     gene            2129..3001
                     /locus_tag="VFN42_09005"
     CDS             2129..3001
                     /locus_tag="VFN42_09005"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HET8996793.1"
                     /translation="MGDFAHPEFLVETGWLAQHLDDPDVVVLDCTTHLIPDPKSTYQV
                     VPGQEDFERGHIPGAQFVDMAQDVSDTSQQLRFMRQSPEAFAAAMQRFGVGNRTRVVS
                     YSTANVWWATRLWWLLREFGHDNAAVLNGGWQKWQREARPFATGPAQPRAAGDFRVHQ
                     VRNLMVGKEAVQRAIGDGAVCTINALQPQQHAGSGGNSYGRPGRIAGSVNLPAAHLLD
                     PDTNTFLPADELRRRFGAVGALDRPVITYCGGGIAASADALALVMLGHPDVKLYDASL
                     SEWAKDPALPMETD"
     gene            3088..>3673
                     /locus_tag="VFN42_09010"
     CDS             3088..>3673
                     /locus_tag="VFN42_09010"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8996794.1"
                     /translation="MPPDGPAQQHSGSLADAAPTLPRRPAVRRSLVLAACLMATFMAA
                     VESTIVATAMPTIVADLGGFGLFSWVFAVYLLTQAIAIPIYGRMADIYGRRKVFYVGV
                     GVFLSGSTLCGFAPSMVWLVLFRALQGFGAGGVQPIAMTICGDIYTPSERAHVQGLIS
                     SVFGVSAVVGPTLGAFLVEHVAWPVVFWVNLPVGA"
BASE COUNT          598 a         1179 c         1297 g          599 t
ORIGIN      
        1 acattggcag accctcgaaa tggctgggca ggacgaaaat atctgctgcg gccaacagcg
       61 ccgccacgtc ctcccggtag ccgagcagac gcagccggtc gcccaggccg gcattcgcga
      121 gcagcgcggc catgtcgccc ccgcggtcgg actgcagccg ctcgccgacc acccaaagtt
      181 cggcgtcggg caccgcccgc atggcggcgg ccagttcggg atagcccttc aggcggacca
      241 gccgggacac ggcgatcacc acgggacgcg tctccggcgt gccgagtgcc gcccggacgc
      301 gtgcgcgcgc ctcagggtct ggccggaacc gctgcgggtc gcggccattg ccgaccgcca
      361 tggcggtgcg ggaaaagcgc aggcggcggg catccgcggc ctcgctctcc gccaccgtga
      421 gatagatgtc ggtcagcggt gcggccgcgc attccatcag caggccagcg ctgctgtgca
      481 gccaggagcc ttgctgattg aacaggaatc cgtggcaggt ataggccacg cgcggcaccc
      541 cggcgatgcg cgccgcgacc cgcgcgagga agccgctgat cggcatgtgg gcatgcacca
      601 tgtccggccg ctctgcccgc atcaccgcga ccaggtggcg cagggcgcgc agatgcgcca
      661 acggcgagat ccgccgttgc agcggcacgg tgatcacccg gaacccttct gcccggaccg
      721 cgtccagcag gcggccatcc gcacagacac cgattacgtc atgcccgcgc gcccgcgcgc
      781 cgcgcatcag cggcagcagg aactggcgga gggaaaagtc cacgttggtg atttcaagga
      841 tcttcatgcg cggcgctgtc atggcctcgc cgggcaggtc gtgcaactcc gccggatggc
      901 ctgcctgtcc cagccgtgac agagtggggc caatcaaccg ggggagatcg catccaatgg
      961 cgaggcttgc gggcaaaatt gcactgatca ccggcggcgg caccggcatc ggcaggacca
     1021 ccgcgctgct gttcgcccgc gagggcgccc gcgtggcggt ggccgagatc gaccgggcgg
     1081 cgggggaggc gacagcgcac ctggccggta acggcgccat ctgcatccgc accgatgtga
     1141 ctgatgaagc cagcatgcgg gccgccgtgc gcggcaccgt gcagcatttc ggcgggctgc
     1201 acgtgctgca caacaatgcc ggcggctcta caccgcagga caacacggtg gtggaagcgc
     1261 cggtggaaga gttctggcgc gtcatcaaac tggacctgtt cggcaccttc ctgggatgcc
     1321 gttttggtat tccggcgatc atcgaatccg gcggtggttc ggtgatcaac atgtcatcca
     1381 acgttgcgct gatggggatt gcgggacgcg actgctacac cgcggcgaag gggggcgtcg
     1441 cggcaatgac ccgctccatg gcagtcgagt tcgccccgca gcatgtgcgg gtcaatgcga
     1501 ttgcaccctc cgctaccatg accgagcggg tgcgcaagct ggtggccggc aatgcggcgc
     1561 tggagcgcat ggcgcagtcg catctgctgg gcctgatcga gccggaggat gtcgccaacc
     1621 tggcggtgtt cctggcttcc gatgaatcgc ggatggtgac cgggcatatc tacccggtag
     1681 acagcggcgt aacgatttcc tgagggggcg gacatgaaga tcaagcgggt ggagcatatc
     1741 gccatcgccg tggactcgct ggaccggtcg gcaggcttgt ggcgcgatgc attcgggctt
     1801 gacgtggaat acgaagagac gatcggcgag acccggctgg cgatgctgcc ggtcggcgaa
     1861 agttacctgg agctgctgga agggcaggga ccggaatccg gcgtctccca gtggatcggc
     1921 gagaagggca ccggcctgtt ccatatctgc ttcgaggtgg aggatatcga cggcgcattg
     1981 gccgaactga aggcaaaggg cgtgaagttg cgcgacgaga ctccgcgcat cggtcatggc
     2041 ggcgcgcgca ttgccttcct ggatccagct tccaccggca acgtactggt cgaactggcg
     2101 gagctgccgg ccgcgggagg gcactgacat gggcgatttc gcgcatccgg aattcctggt
     2161 ggaaaccggc tggctggcgc agcacctgga tgatccggac gtggtggtgc tggactgcac
     2221 gacgcacctg attcccgatc cgaagagcac ctatcaggtg gtgcccggac aagaggattt
     2281 cgaacgcggc cacatccccg gcgcgcagtt cgtcgatatg gcacaggacg tatccgacac
     2341 gtcgcagcaa cttcgcttca tgcggcagag cccggaggcg ttcgccgcgg cgatgcagcg
     2401 cttcggggtc ggcaaccgca cgcgcgtggt cagctacagc acggcgaacg tgtggtgggc
     2461 gacacggctg tggtggctgt tgcgcgagtt cggtcacgac aatgccgcgg tactgaacgg
     2521 cggctggcag aaatggcagc gcgaagcccg gccgttcgcc acgggacccg cgcagccgag
     2581 ggccgcgggc gacttccgcg tgcatcaggt gcgcaacctg atggtcggca aggaggcggt
     2641 gcagcgcgcg atcggcgacg gcgctgtgtg tacgatcaac gccctgcagc cgcagcagca
     2701 cgccggaagc gggggcaaca gctatggccg cccgggacga attgccggca gcgtcaatct
     2761 gccggcggcg catctgctgg atccggatac caacaccttc cttccggccg acgaacttcg
     2821 gcggcgcttc ggcgccgtgg gggctctgga ccgcccggtg atcacctatt gcggcggtgg
     2881 aatcgcggcg agcgcggatg cgctggcgct ggtgatgctg gggcacccgg acgtgaaact
     2941 gtatgatgcc tcgctgtcgg aatgggcgaa ggacccggcg ctgccgatgg aaacggacta
     3001 gcgagaggac gcgaagacgg ggactcgcgc accgttcggc ggtgggcatc agttgtcatt
     3061 ttcccggctg ggtgacagga ataccgcatg ccccccgacg gacctgccca gcagcattcc
     3121 ggcagccttg ccgatgccgc ccccaccctg ccgcgccgcc ccgccgtccg ccgctccctg
     3181 gtgctcgccg cttgtctgat ggccaccttc atggccgcgg tggagagcac catcgttgcc
     3241 accgccatgc cgaccatcgt tgccgatctc ggtggcttcg gcctgttcag ctgggtgttc
     3301 gccgtctacc tgctgaccca ggcgatcgct atcccgatct atggccgcat ggcggacatc
     3361 tacggccgcc ggaaggtgtt ctatgtcggg gtcggcgtgt tcctaagcgg ttccaccctg
     3421 tgcggctttg cccccagcat ggtctggttg gtgctgttcc gagcgctgca agggtttggt
     3481 gcaggcgggg tgcagccgat cgcgatgacg atctgcggcg acatctatac gccgtcggag
     3541 cgcgcgcatg tacaggggct gatctcctcc gtcttcggcg tgtctgccgt ggtcggcccc
     3601 acgctcggcg ccttcctggt ggagcatgtc gcctggccgg tggtgttctg ggtgaacctg
     3661 ccggtcggcg ccg
//
LOCUS       DASSZH010000417         2347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_862162, whole genome shotgun sequence.
ACCESSION   DASSZH010000417 DASSZH010000000
VERSION     DASSZH010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862162"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VFN42_09015"
     CDS             complement(<1..361)
                     /locus_tag="VFN42_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HET8996795.1"
                     /translation="MTEPCDLTAIEARRLIGEKRLSAASLLESCIARIEAVDPAVNAM
                     VARDFDRARAAARQADDAVARGGPLGPLHGLPIGIKDLQRTAGLRTTRGSPLFRDDVP
                     QQDERIVAAVRAAGAIVL"
     gene            553..1431
                     /locus_tag="VFN42_09020"
     CDS             553..1431
                     /locus_tag="VFN42_09020"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HET8996796.1"
                     /translation="MAIGIRPFQAGLGADITGVDIRAPLSPADRDQILAAWHNHLVVR
                     FRGQPMSDEQHMAFTRNFGELEFAAEKLVERTYGVTSDTANRTGVPPQIAVISNIVEN
                     GKPIGALGSGEAFWHTDSSFVDVPPAGSFLRAIEVPPRGGATYFLNMYAAYDTLPDDV
                     QHRIADLQAIHPMTHSSSGKPRKGFENITDVTQVPGPRHPLVRTHPDTGRKCLFLGRR
                     LNAYVCGLPVEESEALLDRLWAHATQEKFTWRQEWQVGDLIIWDNRCAMHRRDPFDPA
                     ARRLMHRTQTKGTRPV"
     gene            1619..1882
                     /locus_tag="VFN42_09025"
     CDS             1619..1882
                     /locus_tag="VFN42_09025"
                     /inference="COORDINATES: protein motif:HMM:NF018343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1150 family protein"
                     /protein_id="HET8996797.1"
                     /translation="MNMRLIGAGYEEFDGLSRAAAALRRMSAEQFLQLGVDQVAYVRA
                     AQHDGTPFFLVHGADGVPLASVDALEDAVEMAAEHGFEFVTVH"
     gene            complement(2115..>2347)
                     /locus_tag="VFN42_09030"
     CDS             complement(2115..>2347)
                     /locus_tag="VFN42_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019084935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET8996798.1"
                     /translation="EVGLSGEVRQVAQAEARLKEAAKLGFDAATLPRRLARGNRAIAA
                     PEGLRLEEIGHIADLVTRFTPEALAEAEAATG"
BASE COUNT          398 a          760 c          812 g          377 t
ORIGIN      
        1 ccagcacgat cgcaccggcg gcgcgcaccg ccgcgacaat gcgctcgtcc tgctgcggca
       61 catcatcgcg gaacagcggg ctgccgcggg tggtgcgcaa cccggccgtg cgctgcagat
      121 ccttgatgcc gatgggcagc ccatgcagcg gaccaagcgg tccgccgcgc gccacggcgt
      181 cgtccgcctg ccgggccgca gcgcgggcgc ggtcgaaatc acgcgccacc atggcattca
      241 ccgcggggtc cacggcttcg atgcgggcga tgcagctttc cagcaggctg gcggcggaca
      301 ggcgcttctc gccgatcaac cggcgggctt cgatggcggt cagatcacac ggttcggtca
      361 tggcggcctt cacggggtgg cgagtgcggc accatagggt gaccacaccg atgtctcaat
      421 gatgcgcgac cagtgctgca ccggaatggc gttgcgttga cgccggaagc cggcgaagcc
      481 taggttttgt gcacccaagg aacgccggct gcagcgccgg cgataacacc gtgaccgggg
      541 agtgacgtgc caatggcgat cgggatccgc cccttccagg cagggttggg cgccgacatc
      601 accggcgtgg atatccgggc accgttgtcg ccggccgatc gcgaccagat cctcgccgcc
      661 tggcacaatc atctggtggt gcggttccgc ggccagccga tgagcgacga acaacacatg
      721 gcgttcaccc gaaatttcgg cgaactggaa ttcgccgcgg aaaagctggt ggaacgcact
      781 tatggcgtga cctccgacac cgcgaaccgt acgggtgtac cgccgcagat cgcggtgatc
      841 tccaacatcg tcgagaacgg caagccgatc ggtgcattgg gctccgggga ggcgttctgg
      901 cacacggaca gttccttcgt ggacgtgccg cccgccggca gttttctgcg cgccatcgaa
      961 gtgccgccgc gcggtggagc gacgtatttc ctcaatatgt atgcggcgta tgacaccttg
     1021 ccggatgatg tgcagcatcg gatcgcggat ttgcaggcga tccatccgat gacgcattcc
     1081 agcagcggca agccacgcaa ggggttcgag aacatcaccg atgtgacgca ggtaccgggc
     1141 ccgcgtcacc cgctggtgcg cacgcatccg gacaccggcc gcaagtgcct gttcctggga
     1201 cgccggctga atgcctatgt ctgcgggctg ccggtggagg agagcgaggc attgctcgac
     1261 cggctctggg cgcatgcaac gcaggagaaa ttcacctggc ggcaggagtg gcaggtgggc
     1321 gacctgatca tctgggacaa tcgttgcgcg atgcaccggc gcgacccgtt cgatccggcg
     1381 gcccgccgcc tgatgcatcg gacgcagacg aagggaacgc ggccggtctg aaggcgttct
     1441 cacgcattga gccgccggaa agggcgtgac agtcgcggcg gcgtgtcccg gctttcggaa
     1501 actacgtacg gggacaagcg cattgcggac ggggcagtgg cgatgcttgc ttggtgccgg
     1561 caagccccgc caccgacacg caaggatggc ggtatgaccc gatcgaagga ccagcaccat
     1621 gaacatgcga ctgatcggcg caggctatga ggaatttgac ggcctgagcc gtgcagcggc
     1681 ggcgttgcgc aggatgagcg cggagcagtt tctgcaactc ggtgtcgacc aggtggccta
     1741 tgtgcgtgcc gcccagcatg acggcacgcc gttcttcctg gtccacggcg ccgacggagt
     1801 tccgctcgcg tcggtggacg cgctggagga tgcggtggaa atggccgcag agcacggctt
     1861 cgaattcgtc acggtccatt gacgagggga gcgtgtcgtg tcggtgattg caattgaagc
     1921 cgatccacag ggcgcaacac gggtgttctt cgattgccgc gatgcgccgc cggaccccgc
     1981 cgatgaacaa tggatcgaac gcgccatggc cgcggccttg gcaccagatc gtgtgatgcc
     2041 tgcggcgcac gctgccgaaa ccgcattctg ctgctgaacg ccgcaagacc cggatgaccg
     2101 gccggcgctg cgcgtcagcc ggtagcggct tccgcctcgg ccagtgcttc cggcgtgaag
     2161 cgcgtcacca ggtcggcgat gtggccgatc tcctccagcc gcagcccttc cggcgccgcg
     2221 atcgcccggt tgccgcgcgc cagccggcgc ggcagggtcg cggcgtcgaa gcccagcttg
     2281 gcggcctcct tcagccgcgc ctcggcttgc gcgacctggc gtacctcgcc ggacaggccg
     2341 acctcgc
//
LOCUS       DASSZH010000418         2285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1612328, whole genome shotgun sequence.
ACCESSION   DASSZH010000418 DASSZH010000000
VERSION     DASSZH010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612328"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VFN42_09035"
     CDS             <1..560
                     /locus_tag="VFN42_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HET8996799.1"
                     /translation="GLFIGGLLPYLFGAMGMTAVGRAAGSVVVEVRRQFREIPGIMQG
                     TAKPEYGRAVDMLTRAAIKEMLIPSLLPIGAPILLWIVIDIIAGRAAAFASLGAMLLG
                     TIVTGLFVAISMTSGGGAWDNAKKYIEEGNHGGKGSDAHKAAVTGDTVGDPYKDTAGP
                     AVNPMIKITNIVALLLLAILAGWLH"
     gene            754..1071
                     /locus_tag="VFN42_09040"
     CDS             754..1071
                     /locus_tag="VFN42_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996800.1"
                     /translation="MPDTPVAGSHGPSYDPLMEARVSRLEEEMREEKAARVRLEAAVL
                     EIKTILTTVLPHLATKADIAVLRAEMRAELAEKPSKTYMWGVLAVLLTAYGCGLAALA
                     VLR"
     gene            complement(1239..>2285)
                     /locus_tag="VFN42_09045"
     CDS             complement(1239..>2285)
                     /locus_tag="VFN42_09045"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8996801.1"
                     /translation="GVTALLQTPVGYLVDSYGARRFLISGALLMSLSIAAMGFATSFW
                     QILLLALLSGIGNSVFHPADYAIISGSVDKDRMGRAFALHTFSGNLGFASAPPIMALL
                     IVAIGWRTSVMLVGAVGVLVAAAIVLQSGILNDGSVVELKQAAPSARSLLLSRTILLF
                     FGFYLLGSMAATGVQAWLITVLHTVKGLDLAAASAALTGYMVGTTLGVLAGGWFADAY
                     KSRLMGCTLATLVVAAVLLAGVDALSLPGLAAIGVMFLAGLLVGTSRTPRDMMLKEAA
                     PQGQIGKVFGFVSAGLPLGSALTPVPFGFLIDRGHPELILVLVAALLVLSLFFVTAAR
                     SSARAAVVIPAVAE"
BASE COUNT          412 a          774 c          761 g          338 t
ORIGIN      
        1 tcggcctgtt catcggcggc ctgctgccct acctgttcgg cgcgatgggc atgaccgcgg
       61 tcggccgcgc agccggctcg gtggtcgtgg aagtgcggcg gcagttccgc gagatccccg
      121 gcatcatgca aggcacggcc aagccggaat acggccgcgc cgtggacatg ctgacgcggg
      181 cggcgatcaa ggaaatgctg atcccatcgc tgctgccgat cggtgcgccg atcctgctgt
      241 ggatcgtcat cgacatcatc gccggccggg cggcggcttt cgcctcgctg ggcgcgatgc
      301 tgctgggcac catcgtaacc gggctgttcg tggcgatctc gatgacctcg ggcggcggcg
      361 cctgggacaa tgcgaagaag tacatcgagg aaggcaacca cggcggcaag ggctccgacg
      421 cacacaaggc ggcggtgacc ggcgataccg ttggcgaccc gtacaaggat acggcgggac
      481 cggcggtgaa cccgatgatc aagatcacca acatcgtggc gttgctgctg ctggcgatcc
      541 tggcgggctg gctgcattag gcagtcgtca gaagccagtt cgtcggctct cacaggcggc
      601 cccgggcgca agtccggggc cgctggcgtt tgcggggctt tgcgctgcgg cggaacctga
      661 ccttcgatct cctggcctct taccggcccg aagttcagga ctttgtttac ctgtgattgc
      721 gagtatcagc gcatcacccg gagctggacg ccgatgcccg acacaccggt tgcaggatcg
      781 cacggccctt cctacgatcc actgatggaa gcacgtgttt cccggctgga ggaagagatg
      841 cgggaagaaa aggccgcacg ggtccggttg gaggcagcgg tcctggagat caagacgatc
      901 ctgacgacgg tgctgccgca ccttgccacc aaggcggaca tcgccgtgct gcgtgcggaa
      961 atgcgtgcgg aactcgccga gaagccgagc aagacatata tgtggggcgt gctcgcggtt
     1021 ctgctcacgg cctatggctg cgggctggcg gcgctggcgg tgttgcgata gccacgatcc
     1081 ggcagtccgt cgttgaccgg ccatagcgcc tccaacggca tggcatcgcg atgcagggca
     1141 cgatggtgac gccgatttcc gcatcgctgt tgccctgagc gacgatggtg tccggatcgc
     1201 cgccgcgccg tgcggatgga cttcggccgg gccggcccct actccgccac tgcgggtatc
     1261 accaccgccg cccgtgcgct ggaccgcgcc gcggtgacga agaacaggct cagcaccagc
     1321 aatgccgcca ccaggaccag gatcagttcc ggatggccgc gatcgatcag gaacccaaag
     1381 ggcaccgggg tcagagcgct acccagcggc aggccggcgc tgacgaagcc gaacaccttg
     1441 ccgatctggc cctgcggcgc cgcttccttc agcatcatgt cgcgcggggt gcggctggta
     1501 cccaccaata gcccggccag gaacatcacc ccgatcgccg ccaggcccgg cagactcagc
     1561 gcatcgacgc cggccagcag caccgcggca accacaagcg tcgcgagcgt gcatcccatc
     1621 agccggctct tgtaggcatc ggcgaaccag ccgccggcca gcacacccag cgtggtgccc
     1681 accatgtaac cggtcagcgc cgcggaggcc gccgccaggt ccagcccctt caccgtgtgc
     1741 agcacggtga tcagccaggc ctgcacgccg gtcgccgcca tcgaccccag caggtaaaaa
     1801 ccgaagaaca gcaggatcgt gcggctgagc agcaggctgc gcgcgctggg cgccgcctgc
     1861 ttcagctcga ccacagaccc gtcgttcagg atgccgctct gcagcacgat ggcggcagcc
     1921 accaggacac cgaccgcccc caccagcatc acgctggtgc gccagccgat ggcgacgatc
     1981 agcaacgcca tgatcggcgg cgcggaggca aagccgagat tgccgctgaa cgtgtgcagc
     2041 gcgaaggcgc ggcccatgcg gtccttgtcc accgaaccgc tgatgatcgc gtaatccgcc
     2101 gggtggaaca cggaattgcc gatgccggac agcagcgcca gcagcagtat ctgccagaag
     2161 ctggtggcga agcccatcgc cgcgatcgac agcgacatga gcagcgcgcc gctgatcagg
     2221 aaacgccgcg cgccgtagct gtccaccaga tagccgaccg gcgtctgcag cagcgccgtc
     2281 acccc
//
LOCUS       DASSZH010000419         3737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_827970, whole genome shotgun sequence.
ACCESSION   DASSZH010000419 DASSZH010000000
VERSION     DASSZH010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827970"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VFN42_09050"
     CDS             <1..507
                     /locus_tag="VFN42_09050"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HET8996802.1"
                     /translation="PPDAEGRLQARGASNFVGYLKHPEWFATDADGWFDTGDLARIDR
                     DGYVRITGRTKDVIIRGGENVPVVEVEGLIYRHPAIQEAAVVAMPDPRLGERACAFVV
                     LRDGASLSFPELVSFLEEQRLARNYLPERLEILPGMPKTPSGKIQKVVLRGMAKNLTP
                     QPLPKPTA"
     gene            527..1288
                     /locus_tag="VFN42_09055"
     CDS             527..1288
                     /locus_tag="VFN42_09055"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET8996803.1"
                     /translation="MFHLQVAGGIATIIMDRAPVNAMSEAWVAGFHTVLDGLDARDDW
                     SVLHLRSALKVFSAGADLKEMRERFAAPDGLERQLATIRGYQALFARIESLPRVTLAE
                     ISGAALGGGFEVTLACDLRIAAREVKVGLPELRLGLLPGAGGTQRMTRLAGRPAALRL
                     ILGAEQISGEQAEQLGLVQWAVPAAELTDTARQIAERYAALPAHAAQAAKACIAAAAT
                     PGMDGFTEEVAQTSHLLQTPRTRELIGAFLAGTNR"
     gene            1356..2021
                     /locus_tag="VFN42_09060"
     CDS             1356..2021
                     /locus_tag="VFN42_09060"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET8996804.1"
                     /translation="MEDSALVERRRAQLTQAAIACFSQNGYHSTTIRDVAERAEVSIG
                     LIYQYVGDKEDLLFLALQEVLQTYERQIPLALQGIDEPLARLCTAVRTHCRINAAAVD
                     ATVLAYRETKSLRRDRRDAIKQKELKANALIAACVRDCVESGLFSEIDTDLFVYQLVM
                     FSHTWALKAWHFASQMDVDTYVDRGLHLLLRGVLTAAGERGLRRLEARPSAAGVRRGR
                     RQA"
     gene            complement(1983..2423)
                     /locus_tag="VFN42_09065"
     CDS             complement(1983..2423)
                     /locus_tag="VFN42_09065"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HET8996805.1"
                     /translation="MNKRLMHALSALNAWVYRASGGRLLGRFSSGAPVCLLTTMGRRS
                     GQTRTVPLLYLPDGADFIVVASQGGAPQHPGWYLNLQANPRAELQAGRQRFPVIGHVL
                     DAAERARFWPKLVAIYPAYQTYQQRTTREIPLLRLSPAAPDAGG"
     gene            2469..2840
                     /locus_tag="VFN42_09070"
     CDS             2469..2840
                     /locus_tag="VFN42_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HET8996806.1"
                     /translation="MDRRQSGRRAHRRGVDAEAAACAALERDGWCVLARRLRTGAGEV
                     DVVAQRDGLLAIVEVKARPRLADAAVALAPRQQARLLAAASVILAEHPGWGAKGVRFD
                     LLLVDAGGRIRRIADAFRADT"
     gene            complement(3528..>3737)
                     /locus_tag="VFN42_09075"
     CDS             complement(3528..>3737)
                     /locus_tag="VFN42_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET8996807.1"
                     /translation="LLHLPPYSPELNPVENVWQFLRQNFLSNRVFDSYEAIVDACCDA
                     WNALMAMPARLRSICERHWAKTLVA"
BASE COUNT          628 a         1215 c         1301 g          593 t
ORIGIN      
        1 ccgcccgatg ccgagggtcg tttgcaggca cgcggcgcca gcaacttcgt cggctacctg
       61 aagcaccccg agtggttcgc caccgacgcg gatggctggt tcgataccgg cgacctcgcg
      121 cgcatcgacc gcgacggcta cgtgcgcatc accggccgca ccaaggacgt gatcatccgc
      181 ggcggcgaga acgtgccggt ggtggaagtg gaaggcctga tctaccgcca tcccgccatc
      241 caggaggccg ctgtcgtcgc catgcccgac ccgcgtctgg gcgagcgtgc ctgcgcgttt
      301 gtggtgctgc gggacggcgc cagcctgagc tttccggaac tggtgagctt cctggaggag
      361 cagcgcctgg cgcgcaacta cctgccggaa cgcttggaga tcctgcccgg catgccgaag
      421 acacccagcg gcaagatcca gaaagtggtg ctgcgcggta tggcgaagaa cctcacgccg
      481 caaccactgc ccaagccgac cgcctgatca gcacaggagc atcgcgatgt ttcatcttca
      541 ggtcgccggc ggcatcgcca ccatcatcat ggaccgcgcc ccggtgaacg cgatgagcga
      601 agcctgggtg gcgggcttcc acacggtgct ggatggcttg gatgcgcgcg acgactggtc
      661 ggtgctgcac ctgcgcagcg cgctgaaggt gttctccgcc ggggccgatc tgaaggagat
      721 gcgggagcgc ttcgccgcgc ccgacgggct ggaacgccag ctcgccacca tccgcggcta
      781 ccaggcactg ttcgcgcgca tcgaatccct gccgcgggta acgctggcgg agatctccgg
      841 cgccgcgctg ggcggcgggt tcgaggtgac gctggcctgc gacctgcgca tcgcggcgcg
      901 cgaggtgaag gtcgggctgc ccgagctgcg cctgggcctg ctgcccggcg ccggcggcac
      961 gcagcgcatg acgcggctgg cgggacggcc ggcggcgctg cggctgatcc tgggcgccga
     1021 acagatcagc ggcgagcagg ccgaacagct cggcctggtg caatgggcgg tgccagcggc
     1081 ggaactcacg gacaccgcgc gccagatcgc cgaacgctac gcggcgctgc cggcacatgc
     1141 ggcgcaggcg gcgaaagcct gcatcgcggc ggcggccaca cccggcatgg acggcttcac
     1201 cgaggaggtg gcgcagacca gccatttgct gcaaacgccc cgcacgcgcg aactgatcgg
     1261 cgcgttcctg gccggcacca accgctgatc ggataaggaa gacgacactg gcacgcgcat
     1321 ccaaggcggc ctcggccatc gcgatcaaga ccacggtgga ggattccgca ctggtggaac
     1381 gccgccgcgc ccagctcacc caggcggcga tcgcctgctt cagccagaac ggctaccact
     1441 ccaccacgat ccgcgacgtg gcggaacggg cggaagtgag catcgggctg atctaccagt
     1501 atgtgggcga caaggaggac ctgctgttcc tcgccttgca ggaggtgctg cagacctacg
     1561 aacggcagat cccgctggcc ttgcagggca tcgacgagcc gctggcgcgg ctgtgcaccg
     1621 cggtgcgcac gcattgccgg atcaatgccg ccgcggtgga tgcgacggtg ctggcatacc
     1681 gggagaccaa gtcgctgcgg cgggaccggc gcgatgcgat caagcagaaa gagctgaagg
     1741 ccaacgcgtt gatcgccgcc tgcgtgcgcg actgcgtcga atccgggctg ttcagcgaga
     1801 tcgacaccga cctgttcgtc taccagttgg tgatgttctc ccacacctgg gccttgaagg
     1861 cgtggcactt cgccagccag atggacgtgg acacgtacgt ggatcgcggc ctgcacctgc
     1921 tgctccgcgg tgtgctgacc gcggcgggcg agcgcggcct gcgtcggctg gaggcgcgtc
     1981 cgtcagccgc cggcgtccgg cgcggccggc gacaggcgta gcagcggaat ttcccgcgtg
     2041 gtgcgctgct gatacgtctg gtacgccgga tagatcgcca ccagcttcgg ccagaaccgc
     2101 gcccgctccg ccgcatccag cacgtggcca atcaccggga accgctgtct gccggcctgc
     2161 agctctgcgc gcggattggc ttgcaggttg aggtaccagc ccgggtgctg tggggcgccg
     2221 ccctgcgacg ccaccacgat gaagtctgca ccgtccggca ggtacagcag aggcaccgtg
     2281 cgggtctggc cggagcggcg gcccatcgtg gtcaacaggc acaccggcgc acccgaggaa
     2341 aaccggccca gcagccgccc gccgctggcg cggtagaccc aggcgttgag ggcgctgagc
     2401 gcatgcatca ggcgcttgtt catcgcgttc accaaggctt agcggttggc gccgatagtg
     2461 ccaccacgat ggaccgtcgc caaagcggcc gccgcgccca taggcgtggc gtcgatgcgg
     2521 aagctgccgc ctgcgccgcg ctggaacgcg acggctggtg cgttctggcg cgccggctgc
     2581 gcaccggggc aggggaggtg gatgtggtgg cgcagcgcga cgggctgctg gcgatcgtgg
     2641 aggtgaaggc gcggccacgc ctcgcggatg cggccgtggc cctggcgccg cgccaacagg
     2701 cgcgcttact cgcggcggct tcggtgatcc tggcggagca tccgggctgg ggcgccaagg
     2761 gcgtgcgctt cgacctgctg ctggtggatg ccggcgggcg catccgccgg atcgccgacg
     2821 cgtttcgggc ggacacgtga cgccgagtgc cggcatgcgg ccttgccggc gtaggttcga
     2881 aacctaagca aggaattcga cccgcagata aacgcacgca cggcccggcg gcagcctgaa
     2941 tctgctgtgc cggcgtttgc ccccaagtgg tgcgctttga tgactcgccg aaacactcgg
     3001 gttcagcctg taaaccgttt gggaagattt ctctgacatc atcggcgcgc accgtgaggc
     3061 agcgtcgatg gagcagggcc cgcttgctgt cgaccagatc tcggagcgcg tcatcggctg
     3121 cgcgatccgg gtgatcaatg aactcggcac tggattcctt gagacagtcc acgagtacgc
     3181 cttggcgcac gacttgcgca aggctggcct gcgcgtctcg caacagcagg cggcacggtc
     3241 atgtatgatg atgtcaatgt cgagattata ccatcgatct tctggttgaa gacacggtta
     3301 tcgttgcacg gaaggcggtc aaagcgctgg atcgcgtgca tgccgcgcaa tgcatcaaat
     3361 accggaaggc atcggacttc cgcgtcggtc tgttactcaa attcggcagt ccgcgactcg
     3421 aaatccggcg gattgccaac tgacctacgg gtatacggca catctgtgtg catctgtggt
     3481 tctgatgctt tgctgtctcc cgatcagcag cggtaatacc aacggcccta tgcgaccagc
     3541 gtttttgccc aatggcgttc gcagatggag cggagccgcg ccggcatcgc catcagcgca
     3601 ttccaggcat cgcagcaggc gtccacgatg gcctcatagc tgtcgaagac gcggttgctc
     3661 aggaagttct gccgcaggaa ttgccagacg ttttcgaccg ggttcagttc cggggaatag
     3721 ggcggcagat gcagcag
//
LOCUS       DASSZH010000420         4965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1203208, whole genome shotgun sequence.
ACCESSION   DASSZH010000420 DASSZH010000000
VERSION     DASSZH010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203208"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="VFN42_09080"
     CDS             <1..686
                     /locus_tag="VFN42_09080"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HET8996808.1"
                     /translation="ARALDGEPEGLAVLARRQTRGRGTNGRAWQSPAGNLYLSVLLRP
                     EEPARHAGQWSLLAAVALAETLAPLLPDPQALALKWPNDALLHGRKLAGILTETSAGP
                     DGMLSFLVIGFGVNLSEAPQLPDRPTACLAEAASPPAPKIFADHLLAALDTWREVRAR
                     EGFAIVREAWLARGPQAEAPLQVRLGATTQAGRFAGLGPDGSLLLATAAGIRTFVAGE
                     VVAPQPGGA"
     gene            690..1499
                     /locus_tag="VFN42_09085"
     CDS             690..1499
                     /locus_tag="VFN42_09085"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HET8996809.1"
                     /translation="MLLAFDAGNTNVVAAIHDRTSWRGIWRVATDPQRTSDEYAVWLL
                     SLLELRGLKRSDVKRAVIGTVVPAALYHLRRLCRDWFDVEPLVVRSSLDWGFEIRMDN
                     PEEVGADRLLNALAAQARYGGPLVVIDFGTATTFDVVGATGAYLGGVIAPGINLSIEA
                     LHRAAARLPRIGMGRPHKVVGTNTVAAMQSGVFWGYVGLVEHLVARIRAEYGSPLKVI
                     ATGGLAPLMAEGATVIEHIDEDITLEGLRLLAQRNPAPTLHRDRIRPADSD"
     gene            1518..3164
                     /locus_tag="VFN42_09090"
     CDS             1518..3164
                     /locus_tag="VFN42_09090"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003620707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET8996810.1"
                     /translation="MDAATGDLAFLPLGGTGEIGMNLNLYRCGGRWLAVDCGIGFGGA
                     ELPEVDVMMPDPAFIAERRDRLVGLVITHAHEDHLGAVAWLWRSLRCPVYASPFAAAI
                     LRRKLAEVQLLSEVKLHVVPPGGTIDLPPFRLSLLRVAHSTPESHSLVIRTPAGTVLH
                     TGDWKLDPDPLVGPPTDEAGFAALAEEGVLAMVCDSTNAMVEGHSGSEGDVRRSLTAL
                     IRDLRGRVVVTCFASNVARVESIAVAARAAGRSVAVVGRSLRNLEAAARDTGYLPGIP
                     PFLSEDEANDVPDDNLLILVTGSQGEPRSALARIAADTHPRIELGEGDTVVFSSRVIP
                     GNERAIGTVQDALVRRGVRLMTDADHMIHVSGHPARDELRRLYRLVKPRYSVPVHGEW
                     RHLSAHAALAQEAGVTPILMEDGDILSLAPGVPEVVDSAPVGRLVLDGARLVPLQGGV
                     MGARRRMLYNGVAIASVAVDEAGRLRGTPRISAPGLYEADDPETGRIAGEMAEALADL
                     PAPLRRDDAALQDAARTALRRALGRRLQKRPLVDVHLLRV"
     gene            3282..3542
                     /locus_tag="VFN42_09095"
     CDS             3282..3542
                     /locus_tag="VFN42_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1467 family protein"
                     /protein_id="HET8996811.1"
                     /translation="MNWFTGVVLYTLIWWVTLFAVLPFGAAPSPDADPTSGWRGAPTR
                     PMLWRKVIATSLLAAVIWAGFYWVITSDWLSFRSGWLALPND"
     gene            3957..>4965
                     /locus_tag="VFN42_09100"
     CDS             3957..>4965
                     /locus_tag="VFN42_09100"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011251680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET8996812.1"
                     /translation="MRLTRTLAPTLKETPAEAQIVSHRLMLRAGLVRQTAAGIYAWLP
                     LGWRVLQNIARIVREEQDAVGAQEMLMPTIQPAELWRQSGRYDAYGPEMLRIKDRHER
                     EMLFGPTNEEMITDLFRQSVKSYRELPQMLYHIQWKFRDEVRPRFGVMRGREFLMKDA
                     YSFDLDYAGAVVSYRRMMLAYMRTFQRMGVKAIPMEAESGPIGGNLSHEFIILAPTGE
                     SQVWYDAAFEQMDFSSRAVSHESEADLDRFFTQMTTPYAATDEKHDPAAWANVAEKRE
                     GRGIEVGHIFYFGTKYSAPLGLTVAGRDGAPVNPHMGSYGVGVSRLVGALIEAHHDEA
                     GI"
BASE COUNT          722 a         1611 c         1789 g          843 t
ORIGIN      
        1 gtgcccgcgc cctggacggt gagccggagg ggctggccgt gctggcgcgc cggcagacgc
       61 gggggcgcgg taccaatggc cgggcctggc agtcgcctgc cggcaatctc tatttgtccg
      121 tgctgctgcg gccggaggaa ccggcgcgcc atgccgggca atggtcgctg ctggccgcgg
      181 tggcattggc cgagaccttg gcgccgctgc tgccggatcc gcaggcattg gcgctgaaat
      241 ggcccaatga tgccttgctg catggccgca aactggccgg tatcctgacg gaaacgtcgg
      301 ccgggccgga cgggatgctg tccttcctgg tgattgggtt tggggtcaac ctgtcggaag
      361 cgccccaact gcccgaccgc ccgactgcct gcctcgccga agccgcgtcg ccgccggcgc
      421 cgaagatttt tgccgaccac ctgctggccg ccctggacac ctggcgggag gtgcgggcgc
      481 gcgaggggtt tgctattgtg cgggaggcgt ggctcgcacg tgggccgcag gctgaggcgc
      541 cgctgcaggt ccggctcggt gcgacgactc aggcgggccg cttcgccggc ctaggtccgg
      601 acggcagcct gctgcttgcg accgctgccg gcatccgtac gtttgtcgca ggcgaggttg
      661 tggctcccca gccaggaggc gcgtgaccga tgctgctcgc tttcgatgcc ggcaatacca
      721 acgtcgttgc cgcgatccat gaccggacgt cctggcgcgg gatatggcgt gtcgcgaccg
      781 acccgcagcg cacttcggat gaatacgcgg tctggctgct ctcgctgctg gagttgcgcg
      841 ggctgaagcg ctcggatgta aagcgggccg tgattggcac cgtggtgccg gcggcgctgt
      901 atcacttgcg gcgcctctgc cgcgactggt tcgatgtcga gccgctggtc gttcgctcca
      961 gtctcgactg ggggttcgag atccggatgg acaacccgga ggaggttggc gccgaccgcc
     1021 tgctgaacgc gctggccgcg caagcgcgct atggcggacc tctggtggtg atcgacttcg
     1081 gcaccgccac caccttcgac gtggtgggcg cgacgggggc ctatctcggc ggcgtgatcg
     1141 cgccgggcat caatctgtcg atcgaggcgc tgcaccgcgc cgccgcccgg ctgccgcgca
     1201 tcggcatggg ccggccgcat aaggtggtcg gcaccaacac ggtggctgcc atgcagtccg
     1261 gcgtgttctg gggctatgtc ggtctcgtgg aacatctggt ggcgcggatc agggccgaat
     1321 atggcagccc gctcaaggtg atcgcgaccg gggggctggc gcctttgatg gccgagggcg
     1381 ccaccgtgat cgaacacatc gacgaagata tcacgctgga gggactgcgg ctgttggcgc
     1441 agcgcaaccc ggcccccaca ttacaccgtg accggatccg gccggccgac agcgactgac
     1501 gaggatttac accgaacatg gatgcggcga ccggcgatct ggcgttcctc ccactggggg
     1561 ggacgggcga gatcggcatg aatctcaacc tctatcgctg cggcgggcgc tggctcgccg
     1621 tggattgcgg cattggcttc ggcggcgccg aactgccgga ggtcgatgtg atgatgcccg
     1681 accccgcctt catcgccgag cggcgcgacc ggctggtggg ccttgttatc acgcacgcgc
     1741 atgaggacca tctgggcgcc gttgcctggc tgtggcggtc gctgcgctgc ccggtctatg
     1801 cgagcccctt cgccgccgcc atcctgcgcc gcaagctcgc cgaggtgcaa ctgctgagcg
     1861 aggtgaagct gcacgtggtg ccaccaggtg gtaccatcga cctgccgccg ttccgcctca
     1921 gcctgctgcg cgtcgcccat tcgacaccgg aatcgcactc cctggtgatc cgcacgccgg
     1981 ccgggaccgt gctgcatacc ggcgactgga agctggaccc cgatccgctg gtcggcccgc
     2041 ccaccgacga ggccggcttc gccgcgctgg cggaggaggg ggtgctggcg atggtctgcg
     2101 actccaccaa cgcgatggtg gaggggcatt ccgggtcgga gggcgatgtc cgacgctcgc
     2161 tgaccgcgtt gatccgcgat ctgcgggggc gcgtggtggt gacctgcttc gcgagcaacg
     2221 tggcgcgggt ggaatcgatt gcagtcgcgg cgcgcgccgc cgggcgcagc gtggcggtgg
     2281 tgggccggtc cctgcgcaat ctggaggcag cggcgcgcga caccggctat ctgcccggca
     2341 tcccgccatt cctctccgag gacgaggcca acgatgtgcc ggacgacaac ctgctgatcc
     2401 tggtcaccgg cagccagggc gagccgcgca gcgcgctggc gcgaatcgcc gccgacaccc
     2461 atccgcggat cgaactgggc gagggcgaca cggtcgtgtt ctcctcccgc gtcatcccgg
     2521 gcaatgagcg cgccatcggc accgtgcagg acgcgttggt gcgccgcggc gtgcggctga
     2581 tgaccgatgc cgatcacatg atccatgtct ccggccaccc ggcgcgcgac gaactgcggc
     2641 gcctgtaccg gctggtgaag ccgcgctact ccgtgccggt gcatggcgag tggcggcacc
     2701 tctcggccca tgccgcgctg gcgcaggagg ccggggtgac gccgatcctg atggaggatg
     2761 gcgacatcct cagtctcgcc cccggcgtgc cggaggtggt ggacagcgcc cctgtcggcc
     2821 ggctggtgct ggacggcgcg cggctggtgc cgctgcaggg cggtgtcatg ggggcgcggc
     2881 ggcgcatgct ctacaacggc gtggcgatcg cgagcgtggc ggtggacgag gcggggcggc
     2941 tgcggggtac gccacggatc agcgcgccgg gcctgtacga ggcggacgac ccggagacgg
     3001 ggcgcatcgc cggcgagatg gcggaggcgc tggccgacct gccggcgccg ctgcggcgcg
     3061 acgatgcggc gctgcaggat gcggcgcgca cggcgctgcg ccgggcgctt gggcggcggc
     3121 tgcagaagcg gccactggtc gatgtacatc tgctgcgggt ctgagagttt gtgaaggaat
     3181 gccccttgcg accggccgtc cctagcgaaa tcagaaactt gcaaggggca ttccttcgca
     3241 agttcgattt ctttcacaag ctctgagccg gggcgacttc gatgaactgg tttaccgggg
     3301 tcgttctcta cacgctgatc tggtgggtga cgctgttcgc ggtgctgccg ttcggcgccg
     3361 cgccctctcc cgatgccgat cccaccagcg gctggcgcgg tgcgccgacg cggccgatgc
     3421 tgtggcgcaa ggtcattgcg acgagcctgc tggcggcggt gatctgggcc gggttctact
     3481 gggtgatcac cagcgactgg ctgagcttcc gcagcggttg gctggctctg ccgaacgatt
     3541 aggcgccccc gcctgctccg tcggcagtat gcccgctgaa gtggcaaagc catgtacagg
     3601 atggctattt tcttgcccag caggcgaaaa aaccgatgga cgagggaaag aggatcagca
     3661 atcatcacgg ccaggatgag gccctgcctc gtcctgccgt gtgactggcg tttcctccct
     3721 aaacttggcc cgctgcgcct tgggcgcgcg ggccgctttt gtgtctagcg gcaaatggca
     3781 aagttgtcac ctggtttcgg tcgcgaggcg gccgatttca gcgattttat ttcctccgac
     3841 ttttgtgcgg cgcacaattg ttgttggttc agcggaaacg gcgaccgatc cctggcggtt
     3901 gctgccgcgg ttcggtaggg tccgcgcctt gccagttctc gtccggatgg atggtcatgc
     3961 gactgacgcg cacccttgcc cccacgctga aggaaacgcc tgccgaggcg cagatcgtct
     4021 cgcatcgcct gatgctgcgc gccgggctgg tgcggcagac cgcggcgggc atctatgcct
     4081 ggctgccgct cggctggcgc gtgctgcaga acatcgcgcg gatcgtgcgc gaggagcagg
     4141 acgcggtcgg cgcgcaggaa atgctgatgc cgaccatcca gcccgccgag ctgtggcggc
     4201 agagcgggcg ctatgacgcc tacgggccgg agatgctgcg catcaaggac cgccacgagc
     4261 gcgagatgct ctttggtccc accaacgagg agatgatcac cgacctgttc cgtcagtcgg
     4321 tgaagtcgta ccgcgaactg ccgcagatgc tctaccacat ccagtggaag ttccgcgacg
     4381 aggtgcgccc gcgcttcggc gtgatgcgcg gccgcgaatt cctgatgaag gacgcgtaca
     4441 gcttcgacct cgattatgcc ggcgcggtgg tcagctatcg ccgcatgatg ctggcctata
     4501 tgcgcacgtt ccagcgcatg ggcgtcaagg cgatcccgat ggaggccgag agcgggccga
     4561 tcggcggcaa cctgagccac gagttcatca tcctggcgcc gaccggcgag agccaggtgt
     4621 ggtacgacgc ggcgttcgag cagatggatt tctcctcccg cgccgtcagc cacgaatcgg
     4681 aagccgatct cgaccggttc ttcacgcaga tgacgacgcc gtacgccgca accgacgaga
     4741 agcacgatcc cgccgcctgg gccaatgtcg ccgagaagcg ggaggggcgc ggcatcgagg
     4801 tcggccacat cttctacttc ggcacgaaat acagcgcgcc gctcggcctg accgtggcgg
     4861 gccgcgacgg cgcgccggtc aacccgcaca tgggcagcta cggcgtcggc gtctcccgcc
     4921 tggtcggcgc gctgatcgag gcgcatcacg acgaggccgg catca
//
LOCUS       DASSZH010000421         2681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_912980, whole genome shotgun sequence.
ACCESSION   DASSZH010000421 DASSZH010000000
VERSION     DASSZH010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912980"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VFN42_09105"
     CDS             <1..538
                     /locus_tag="VFN42_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631968.1"
                     /note="dGTPase family type 2 subfamily; presumably
                     hydrolyzes dGTP to deoxyguanosine and triphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HET8996813.1"
                     /translation="GLRARLFGPEEIAHLPVVKEALAAARMSSLDVPPPRLRHETIRR
                     VIDAMITDLLAETSRRLAKLDPRDADAVRRSKSKVVAFGPAMAEANQVIKDFLFARMY
                     RHWRVNRMTTKARRLTEEVFRLLHADPSLLPDDWRARAGEGGTARAATIVGDYVAGMT
                     DRYAMDEHRRLTDLSVSG"
     gene            670..2457
                     /gene="argS"
                     /locus_tag="VFN42_09110"
     CDS             670..2457
                     /gene="argS"
                     /locus_tag="VFN42_09110"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003624393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET8996814.1"
                     /translation="MTHDIYARLRDTVLTALRSMVPDLPDDIAARVEVTPARDPAHGD
                     MATNAALLAAKPARQPPAKLAAGLVAFLGEAPEVAEATAAGPGFVNIRLDPSAFRALL
                     PGILRAGEAYGDSRSGSDVRVNVEYVSANPTGPMHIGHCRGAVVGDALANLLTKAGYD
                     VTKEYYINDAGAQVAALAWAAYWRYLQALGTPLSEEEFSEQVPGGLQYRGEYLIPVGE
                     QLAEQYGGELAQPDLGIAAPEVWIDIVRDFTIAAMMQEIREDLLRLGVDQHVFSSERA
                     LVASGAADAAIDWLQTQGLIYEGVLEPPKGKAPEDWEPRAQTLFRATQFGDDVDRPLR
                     KSDGSNTYFANDTAYHADKLNRGHVVLIDILGADHGGYVKRMQAAVQALSGGDAKFEA
                     VLCQIVHIIRGGQPVKMSKRAGSYVTLRDLIEEVGKDAVRFTMLTRKADAQMEFDLDQ
                     VVAQTRDNPVFYVQYAHARCRSVLRAAAEMFDAATTADAALAEIPLDSLAAEPELTLI
                     RRLAAWPRTVDGAAQAREPHRIAFFLYDLAADFHMLWNRGKDDATLRFLQADRPAETL
                     ARLALVAATAVVIRSGLAVMGVEPVEEMR"
BASE COUNT          428 a          890 c          947 g          416 t
ORIGIN      
        1 cggcctgcgc gctcggctgt tcggcccgga ggagatcgcg cacctgccgg tggtgaagga
       61 ggccttggcc gcggcccgca tgtccagcct ggacgtgccg ccgccccggc tgcggcacga
      121 gaccatccgc cgggtgatcg atgcgatgat caccgacctg ctggcggaga ccagccggcg
      181 cctcgccaag ctggacccca gggatgccga tgcggtgcgc cgcagcaagt cgaaggtggt
      241 ggcgttcggc ccggcgatgg cggaggccaa ccaggtgatc aaggactttc tgttcgcccg
      301 catgtaccgc cactggcggg tgaaccgcat gacgacgaaa gcccgccgcc tgaccgagga
      361 ggtgttccgg ttgctgcacg ccgacccgtc gctgctgccg gatgactggc gggcgcgggc
      421 cggggagggg ggcaccgcgc gcgccgcgac cattgtcggc gactacgtgg ccggcatgac
      481 cgaccgctac gcgatggacg aacaccggcg gctgaccgat ctttcagtat cgggctgaga
      541 acaccagagc cggccgcgtg agaacaggta tgcggccgag ctggtagccg tgcgctgggc
      601 cgcattcgcg ttgtcggcgc ggttcgggca tagaggccgc ggccgggaag ccggctccgg
      661 agtgaagcaa tgacccacga catctacgcg cgcctgcgcg acaccgtgct gactgccttg
      721 cgcagcatgg ttcccgacct gccggacgac atcgccgcac gcgtggaagt gaccccggcg
      781 cgcgatccgg cgcatggcga catggcgacc aacgcggcgc tgctcgccgc caaacctgcg
      841 cgccagccgc cggcgaaact ggccgccggg ctggtggcct tcctcggcga ggcgccggag
      901 gtggcggaag ccaccgccgc cggccccggt ttcgtcaaca tccggctgga tcccagcgcc
      961 ttccgtgcgc tgctgccggg tatcctgcgt gccggcgaag cctatggcga cagccgcagc
     1021 ggcagcgatg tccgggtgaa tgtggaatac gtctccgcca accccaccgg cccgatgcat
     1081 atcggccact gccgcggcgc ggtggttggt gacgcgctgg ccaacctgct gaccaaggcc
     1141 ggctatgacg tcacgaagga gtactacatc aacgacgccg gcgcgcaggt cgcggcgctc
     1201 gcctgggccg cttactggcg ctatctgcag gcgcttggca cgccgctcag cgaggaggaa
     1261 ttctccgagc aggtgcccgg cggcctgcaa taccgtggcg agtatctgat cccggttggc
     1321 gagcaactgg ccgaacagta cggcggcgaa ctcgcgcagc ccgatcttgg catcgccgcg
     1381 ccggaagtgt ggatcgatat cgtgcgcgac ttcaccatcg ccgcgatgat gcaggagatc
     1441 cgcgaagatc tgcttcggct cggcgtcgat cagcatgtgt tcagctcgga gcgtgcgctg
     1501 gtggcctccg gcgccgccga cgccgccatc gattggttgc aaacgcaggg gctgatctat
     1561 gagggcgtgc tggagccgcc caagggcaag gcgccggaag actgggaacc gcgcgcgcag
     1621 accttgttcc gcgccacgca attcggcgat gacgttgatc gcccgttgcg caaatccgac
     1681 ggcagcaaca cgtatttcgc caatgacact gcgtatcacg cggacaagct gaaccgcggc
     1741 catgtggtgc tgatcgacat acttggcgcc gaccatggtg gctatgtgaa gcgcatgcag
     1801 gcggcggtgc aggcgctgtc cggcggcgac gcgaagttcg aagccgtgct ctgccagatc
     1861 gtgcacatca tccggggcgg ccagccggtg aagatgtcga agcgcgcggg cagctacgtc
     1921 acgctgcgtg acctgatcga ggaggtcggc aaggacgcgg tccgtttcac catgttgacg
     1981 cgcaaggccg acgcgcagat ggagttcgat ctggaccagg tggtggcgca gacccgcgac
     2041 aacccggtgt tctatgtgca gtacgcacat gcccgctgcc gctccgtgct gcgcgccgcg
     2101 gcggagatgt tcgatgcggc aaccactgct gacgccgccc tggcggagat cccgctggat
     2161 tcgctggctg ccgaaccgga actgacgctg attcgccggc tggctgcctg gccacgcacc
     2221 gtggatggcg ccgcccaggc gcgtgaaccg caccgaatcg cgtttttcct ctatgacttg
     2281 gcggccgact ttcatatgct gtggaaccga ggcaaggacg acgccacgct tcgcttcctg
     2341 caggccgacc gcccggccga aacactggcc cggctggcgc tggtggcagc gacggcggtg
     2401 gtgattcgct ccggcctcgc agtgatgggc gtagagcccg tcgaggaaat gcgctgacgc
     2461 gcggcacgga cgtggcggtc ccaggggagg ccgccatcct cgccgcgagc cgcgccagcg
     2521 gaaagaggcc gcgtcatgtc cgacgatatc agcatcccca gcccgaccta ccgcgtgccg
     2581 cgccaccggg gcgtcgatcc ggccacacgc cggcttgctt tgatcgctgc cggcctgggc
     2641 ggcgcgttgg tggtgattgt cggcggctgg tcgatgctgg g
//
LOCUS       DASSZH010000422         3971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_947544, whole genome shotgun sequence.
ACCESSION   DASSZH010000422 DASSZH010000000
VERSION     DASSZH010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947544"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="VFN42_09115"
     CDS             complement(<1..1110)
                     /locus_tag="VFN42_09115"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8996815.1"
                     /translation="MRLPPALLPLRHQTFRRLWLASVIAWLGTWLQNTGAGWLMTILA
                     PDPLIVAMVQAATIMPVFLLALPGGALADIVDRRVFLIGTQAWTILAATSLAVLTVAG
                     GMTASWLLVLTFAIGIGSALTAPAWSAVVPELVPREDLVGAIALNGIGYNLTRAVGPA
                     LAGFLILLGGTSLAFSLYACSIVAVIGALVMWKRGRRFTGMPREQLISAMRAGVRFVR
                     HTPAIQSAMVRTIAYSMPSAAPWALLPLFVHDRLGLGAGMYGVILGCMGIGGVTSGML
                     LPNVRRYLSRGNTVVVCTLFSCAGMTLLAVSHHWALAGLGMLLFGLGWTSAYATMQAA
                     AQLVCPPWVRARSLAIYQLAQNGALTLGSFAWGWFG"
     gene            complement(1115..2173)
                     /locus_tag="VFN42_09120"
     CDS             complement(1115..2173)
                     /locus_tag="VFN42_09120"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET8996816.1"
                     /translation="MKMPESPGREFAVARQAAIPLSDGITAEQGGEMDQAEQRIDGGE
                     RTSLLCRPVPVPWQIGAGALVAAVVLLIMWLPPWPSVATWRLMPFDLVAVAAGAGAAG
                     LYAGLTGAAIILLYVVAGSVVLQPYPVVHDPAWLTLLGVAIFGVALIAGGLRDLLAQM
                     EQELGGTKRLLAGANRRLASALESERLRSYYDHVTDLPSRRMVIDRFTQGLAQARRNE
                     ALLAMLLLDLNRFREVNEVIGHDAGDEVLRQIGQRLSLVMRREDTVGRLDSDTFIILL
                     TGAADIAGVTAAAQKVADALAEPFVLGSPPREIFVSASLGAAIYPQDGHDWDSLYHHA
                     EEAVWGAKGQAGPSGSGD"
     gene            complement(2170..2934)
                     /locus_tag="VFN42_09125"
     CDS             complement(2170..2934)
                     /locus_tag="VFN42_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HET8996817.1"
                     /translation="MPDRKQRADQMLVDRGLAESRARAQALIIGGKVFSGERRIDKPG
                     TALPSDTPLEVRGQDHPWVSRGGLKLAHALAHFALSPAERVCLDIGASTGGFTDVLLA
                     HSAAKVHAVDVGHGQLAWKLRNDPRVVVHERTNARHLDAAAIGEPVQALVCDASFIGL
                     QTLLPASLSLCGPGAWAVALIKPQFEAGPQHVGSKGVVRDPAVHDAVCSRIRGWWQQL
                     PGWRVLGIVESPITGPEGNKEFLIAALRQVDNPEKQ"
     gene            complement(3217..>3971)
                     /locus_tag="VFN42_09130"
     CDS             complement(3217..>3971)
                     /locus_tag="VFN42_09130"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET8996818.1"
                     /translation="VSVGGGAHSGRALRLGSIVMLNASRLILDKAQRIASHVLEVAPG
                     DLEFADGRFTVAGTDRSLGLFEIARIALERNDLPEELRGKLDAISDETIPEASFPYGC
                     HVCEVEVDPETGVTRIVRYTAVDDVGRAVNPMIVHGQVHGGIVQGVGQAMGEQCVYDP
                     DSGQLLSGSFMDYTMPRADTLPFFTTELSEIPSTTHPLGIRPAGEGGTTPALGVMVNA
                     VVDALSPFDVTHVEMPLTSERVWRAIRDARAG"
BASE COUNT          684 a         1470 c         1282 g          535 t
ORIGIN      
        1 gccgaaccag ccccaggcga aggagcccag cgtcagcgcg ccgttctgcg ccaactgata
       61 gatcgccagc gaccgcgcgc gcacccaggg cggacacacg agctgcgccg ccgcctgcat
      121 cgtcgcatag gccgaggtcc agcccagccc gaacagcagc atgcccagcc cggccagcgc
      181 ccaatgatgc gacacggcca gcagcgtcat gccggcgcag gagaacagcg tgcacaccac
      241 cacggtgttg ccgcggctga gatagcgccg cacgttcggc agcagcatgc cggaggtcac
      301 gccaccgatc cccatgcagc cgaggatcac gccgtacatc cccgcaccca gccccaaccg
      361 gtcgtgcaca aacagcggca gcagcgccca gggcgcggcg gagggcatcg aataggcgat
      421 cgtccgcacc atcgccgact gaatcgccgg ggtgtgccgc acgaagcgca ctccggcgcg
      481 catggcggag atcagttgct cgcgcggcat gccggtgaag cgccggccgc gcttccacat
      541 caccagcgcg ccgatcaccg cgacgatcga gcaggcgtac aggctgaacg ccaggctggt
      601 gccgcccagc aggatcagga agccagccag cgcgggaccg acggcacggg tcaggttgta
      661 gccgatgccg ttgagcgcga tcgccccgac aaggtcctca cgcggcacca gttccggcac
      721 caccgcactc caggccggcg cggtcagcgc agagccgatg ccgatggcga aggtcagcac
      781 cagcagccag gaggcggtca tgccgccggc cacggtaagc accgccaggg aggtcgccgc
      841 cagaatggtc caggcctggg tgccgatcag gaacacgcgg cggtcgacga tgtcggccag
      901 cgcgccgccc ggcagcgcca gcaggaacac cggcatgatg gtcgccgcct gcaccatcgc
      961 cacgatcagc gggtccggcg ccaggatggt catcagccag ccggcaccgg tattctgcag
     1021 ccaagtgccc agccaagcga tgacgctggc cagccaaagc cggcggaagg tctggtggcg
     1081 cagcggcagc agggccgggg gcaagcgcat cgcgtcagtc gccggaaccc gacggccctg
     1141 cctggccctt cgccccccac accgcctcct cggcatggtg gtacaggctg tcccagtcgt
     1201 ggccgtcctg cggatagatg gcggcgccga ggctggccga gacgaagatc tcccgcgggg
     1261 ggctcccgag cacgaagggc tccgccagcg catccgccac cttctgcgcc gccgcggtca
     1321 cgccggcgat gtcggcggcg ccggtgagca ggatgatgaa ggtgtcgctg tccagccgcc
     1381 cgaccgtgtc ctcacgccgc atcaccagcg acaggcgctg gccgatctgg cgcagcacct
     1441 catcgccggc gtcatggccg atcacctcat tcacctcgcg gaaccggttc agatcgagca
     1501 gcagcatcgc cagcagcgcc tcgttgcggc gtgcctgcgc caggccctgg gtgaagcggt
     1561 cgatcaccat ccggcgtgac ggcaggtcgg tgacatggtc gtagtaggag cgcagccgtt
     1621 cgctctccag cgcgctggcc agccggcgat tggcccctgc cagcaagcgc ttggtgccgc
     1681 ccagctcctg ttccatctgc gccagcaggt cgcgcaatcc gccggcgatc agcgcgacgc
     1741 cgaagatcgc cacgccaagc agcgtcagcc aggccgggtc gtgcaccacc gggtatggct
     1801 gcagcacgac agagcctgca acgacataca gcaggatgat cgcggcgccg gtcagcccgg
     1861 catacagccc ggcggcaccc gccccggccg ccaccgccac caagtcgaac ggcatcagcc
     1921 gccaggtggc aaccgaaggc cagggcggca gccacatgat gagcaggacc acagcggcga
     1981 ccagcgcccc cgccccgatc tgccaaggca ccggaaccgg gcggcacaac aggctggtcc
     2041 gctccccgcc atcgatccgc tgctccgcct ggtccatttc cccgccttgc tcggccgtga
     2101 tcccgtcgct tagcggaatg gcggcctgcc gcgctactgc gaactcacgc cccgggctct
     2161 ccggcatttt cattgctttt ccggattgtc cacctgacgc aaggcggcga tcaggaattc
     2221 cttgttgccc tccgggccgg tgatgggact ttccacgatc cccagcaccc gccagccggg
     2281 cagttgctgc caccacccgc ggatgcggct gcagaccgca tcatgcaccg cgggatcgcg
     2341 caccacaccc ttgctgccga catgctgggg tcccgcctcg aattgcggct tgatcagcgc
     2401 caccgcccag gcgccaggcc cacacagcga cagcgacgcc ggcagcagcg tctgcaggcc
     2461 gatgaagctc gcatcgcaca ccagcgcctg gacgggctcg ccgatcgccg ccgcatccag
     2521 atgccgcgca ttggtgcgtt catgcaccac cacacgcgga tcattgcgca gcttccaggc
     2581 gagctggccg tgcccgacat ccaccgcgtg caccttcgcc gcgctatgcg ccagcagcac
     2641 atcggtgaat ccgccggtgg aagcgccgat atcgaggcac acccgctcgg ctggcgacag
     2701 cgcgaaatgc gccagcgcat gcgccagctt cagcccgccg cgcgacaccc aggggtggtc
     2761 ctggccgcgc acctccagcg gtgtgtcgga cggcaacgcg gtgccgggct tgtcgatgcg
     2821 gcgctcgccg ctgaacacct tgccgccgat gatcagtgcc tgcgcgcgcg cgcgcgattc
     2881 cgccaggcca cgatccacca gcatctggtc cgctcgctgc ttgcggtcag gcataagacg
     2941 cgcccgccga acaccatcct ggcgggcgaa ggcccatgcg cgctgacaca aggcgacgac
     3001 cagccgcaca ccgtcgtgac gggcgaaggc ccgtcaccca cgactggctg tgggggactc
     3061 gcaaggcgga ttgcggcctc gaatctcccc cggcaattcg tgggtggcgg cccttcgccc
     3121 gccacgacgg ggtacgcggc atcccgagct gttcagccaa ttggattgta tcctcctgcg
     3181 cccgccagga cgcagtgtgc gtctcctcgc aaatcctcac cccgcccgtg catcccggat
     3241 ggcccgccac acgcgttccg aggtcagcgg catttccaca tgcgtcacgt cgaacggcga
     3301 cagcgcatcg accaccgcgt tcaccatgac gccgagcgcc ggcgtggtgc cgccttcgcc
     3361 ggcaggacgg atgccgagcg gatgcgtggt ggaggggatc tccgacagct cggtggtgaa
     3421 gaacggcagc gtgtcggcgc ggggcatcgt gtagtccatg aaggagccgg acagcagttg
     3481 tccgctgtca ggatcataca cgcattgctc gcccatcgcc tggccgacgc cctgcacgat
     3541 gccgccatgc acctggccgt gcacgatcat cggattcacc gcgcggccga catcgtccac
     3601 cgcggtgtag cggacgatcc gcgtcacacc ggtctccgga tcgacttcca cttcgcagac
     3661 gtggcaaccg tagggaaagc tcgcttccgg gatggtttcg tcgctgatgg cgtccagctt
     3721 gccgcgcagc tcctccggca gatcgttgcg ttcaagcgca atgcgggcga tctcgaacag
     3781 gccaagcgag cggtcggtac cggccacggt gaagcggcca tcggcgaact ccaggtcgcc
     3841 cggcgcaact tccagcacat ggctggcgat gcgctgcgcc ttgtccagga tcagccgcga
     3901 cgcgttcagc atgacgatgc tgcccagccg tagcgcgcgc ccggaatgcg cgccgccgcc
     3961 aaccgagacc c
//
LOCUS       DASSZH010000423         2060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_43985, whole genome shotgun sequence.
ACCESSION   DASSZH010000423 DASSZH010000000
VERSION     DASSZH010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43985"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1936
                     /locus_tag="VFN42_09135"
     CDS             <1..1936
                     /locus_tag="VFN42_09135"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET8996819.1"
                     /translation="FVTERMPVLQAMGWRSEIDAAFGPRFVDSVGVCDVQIADAPQGG
                     FSLELGIEIDGARRPLLPILMRLRERGGIDAARIIDGEVITSLDDGRILRLPAERTRH
                     LLAIVDDLIETASRTTGDSLVMDADAAPAVLDLEDLVTARWQDAATIAAHVERFRAAA
                     ELPEVVVPPSFKASLRAYQQQGVNWLQHLRQNALGGLLADDMGLGKTAQTIAHIVIEE
                     AEGRLDRPVLIVVPTSLVPNWTAELSRFAPHLRVAVLHGLDRHDRRRDLTGVHVVITT
                     YTVLARDIEEMAELPWHIVVLDEAQAIKSPTSKATQAVCRLDTRHRLCLSGTPIENNL
                     GELWSQFAFLMPGLLGQRRHFTRRFRTPIEKDGDPVRRRQLGTRIRPFILRRTKAAVA
                     TELPPKHTILRRITLAPDQRELYETIRSTLHEKVVQGIAARGIRQSQMLVLDALLKLR
                     QVCCDPRLVKLASTQLGGTSSKLDDLLEMLAEMIAEGRRVLLFSQFTTMLDLMKPGLA
                     EAGIPFVELRGDTADRAAPVQRFEAGEVPLFLISLKAGGRGLNLTSADTVIHYDPWWN
                     PAVEDQASDRAYRIGQTKSVFVYKMIAADTVEERILDLQERKAELARIAFSDDGTGVS
                     GMDTDDIDFLFGVAPDRLAA"
BASE COUNT          340 a          682 c          722 g          316 t
ORIGIN      
        1 gttcgtcacc gaacgaatgc cggtgctgca ggcgatgggc tggcgcagcg aaatcgatgc
       61 cgcgttcggg ccgcgcttcg tcgacagcgt cggcgtgtgc gacgtgcaga tcgccgatgc
      121 gccgcagggc ggtttctcgc tggagcttgg catcgaaatc gacggcgccc gccggccgct
      181 gctgccgatc ctgatgcggc tacgagaacg tggcgggatc gacgcggcac ggattatcga
      241 tggtgaggtg atcaccagcc tggacgatgg ccgcatcctc agactgccgg ccgagcggac
      301 ccggcacttg ctggcgatcg tggacgacct gatcgagacc gcgagccgca cgaccggcga
      361 cagcctggtg atggacgccg acgctgcgcc cgccgtgctc gacctggaag acctggtcac
      421 cgcccgctgg caggatgcgg cgacgatcgc ggcgcatgtc gaacgcttcc gcgccgctgc
      481 cgaactgccg gaggtggtgg taccacccag cttcaaggcc agcctgcggg cctatcagca
      541 gcagggcgtc aattggctgc agcatttgcg gcagaacgcg ctgggcggat tgctggccga
      601 cgacatgggc ctgggcaaga cggcgcagac catcgcccat atcgtcatcg aggaggccga
      661 gggccggctg gaccgtccgg tgctgatcgt ggtgccgacc agcctggtgc cgaactggac
      721 ggcggaattg tcccgcttcg caccgcatct gcgcgtggcc gtgctgcacg ggctggatcg
      781 ccacgaccgc cgccgcgacc tgactggcgt acatgtcgtg atcaccacct ataccgtgct
      841 ggcgcgcgac atcgaggaga tggcggaact gccctggcat atcgtcgtgc tggatgaggc
      901 gcaggcgatc aagagcccca cctccaaggc gacgcaggcg gtatgccggc tggacacccg
      961 ccaccggctg tgcctgtccg gcacgcccat cgagaacaac ctgggcgagc tgtggtcgca
     1021 gttcgccttc ctgatgccgg ggctgctggg ccagcggcgg cacttcaccc gccgcttccg
     1081 cacgccgatc gaaaaagacg gcgaccccgt ccggcggcgg cagcttggca cccgcatccg
     1141 cccgttcatc ctgcgccgga cgaaggcggc ggtggcgacg gagctgccgc ccaagcatac
     1201 catcctgcgg cgcatcaccc tggcgccgga tcagcgggag ctgtacgaaa ctatccgctc
     1261 caccctgcat gagaaggtgg tgcaggggat cgccgcgcgc ggcatccggc aaagccagat
     1321 gctggtgctg gatgcgctgc tgaagctgcg gcaggtgtgc tgcgacccgc ggctggtgaa
     1381 gctggcgtcc acgcaactcg gcggcacctc cagcaagctg gatgacctgc tggagatgct
     1441 ggcggaaatg atcgcggaag ggcggcgggt gctgctgttt tcgcagttca ccaccatgct
     1501 ggacctgatg aagcccggcc tggccgaagc cggcatcccg tttgtggagc tgcggggcga
     1561 caccgccgac cgcgccgcgc cggtgcagcg gtttgaggcc ggggaggtgc cgctgttcct
     1621 gatcagcctg aaggccggcg ggcgcgggct gaacctgacc tcggccgaca ccgtgattca
     1681 ctacgatccg tggtggaacc cggccgtcga ggaccaggca tccgaccgcg cctaccggat
     1741 cgggcagacg aagtcggtgt tcgtctacaa gatgatcgcc gcggacacgg tggaggagcg
     1801 catcctggac ctgcaggagc gcaaggcgga actggcgcgc atcgccttca gcgacgacgg
     1861 caccggcgtg tcggggatgg acaccgacga catagacttc ctgttcggcg tcgcacccga
     1921 ccggctggcg gcatgataac gcgcgggacc cgatcgtccg gtccgttacc cttcgcaggc
     1981 ggggcgctgc catcgcgacg gcgtgccgcc tgatctagat catcgcacga cctcctgggc
     2041 cggataacct ccgccgacag
//
LOCUS       DASSZH010000424         2926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1305614, whole genome shotgun sequence.
ACCESSION   DASSZH010000424 DASSZH010000000
VERSION     DASSZH010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305614"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..1186
                     /locus_tag="VFN42_09140"
     CDS             89..1186
                     /locus_tag="VFN42_09140"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HET8996820.1"
                     /translation="MQATAAVFRKVHEPMTIETVELDKPRRREVLVRTVATGVCHSDL
                     HVVDGNGRFPLDRPFVLGHEGAGVVEAVGDDVTAVRKGDHVVACLSGFCGNCPQCLAG
                     HPNLCTGGIVARAESDTPRISQGGQPFRSFAAIGSYASHMLLHENSLVRIDPDLPLDR
                     ACLVGCGVITGVGAALRSAGLQAGQTVAVFGCGGVGLSIIQGARIGGARQIIAVDMFD
                     SKRDMAMHFGATHFVNSSEDDPVKAVRALTGGLGVDQAFEAVGISKLCRQAIESLAIR
                     GTATIVGVLPPDAMIEFPWMAIRPECRVQTSRMGSNQFRSDIPHYLEFYRQGRLNLDD
                     MISRRAPLDGVNEAFRAMKAGEVARTVLMFD"
     gene            1212..2387
                     /locus_tag="VFN42_09145"
     CDS             1212..2387
                     /locus_tag="VFN42_09145"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HET8996821.1"
                     /translation="MLTTTRDMILPTAITGSYPRPLWFDLSLHGRSFKSALGESLFRE
                     QYLDAVAAVINAQEAAGLDIVTDGDSRFDLAVGGKSWFFYPIERLGGIDGHRDTSQGW
                     MGRHNLRPGHILWEVQEAYQPAIVKQKLTRGAWEYTALWQVAQRLTDKPVKFGAICAP
                     ALASMLWNEYYPDNRAMILDLCDIMNAELREMAAAGCPLIQVEEPPHHGRSLRPDCTE
                     EELQFLTDAFNRQLTGVDAEIWVHTCWGNPNQQRLYWEAPSYERAMPYLLQLDADVIT
                     FECASSNGRDLHLFGKYQTDKKIGIGVVNHCTTVVEPPSLVADLIRRALEFIPPERLV
                     ITTDCGFGREGLSRRIAHYKCVSMVQGTNIVRRELGLPEVPIRAADPRLWFAGTTEH"
     gene            2400..>2926
                     /locus_tag="VFN42_09150"
     CDS             2400..>2926
                     /locus_tag="VFN42_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996822.1"
                     /translation="MAKGILLVAFDFTNAHEDEFHDWYDLEHVPERQRVPGFGACDRW
                     ISISNPKQAVASYELDSIDVLQSPAYRAIAGDNLSVWSKRVTAMCTRLLRLEGEQTLP
                     GNQSAPQGAGGLLVNAMSVDPEYEAEFNEWYDHEHIPALSAVPGTLCARRFRDRHGSP
                     RYLALYHLASPDVQAT"
BASE COUNT          503 a          973 c          990 g          460 t
ORIGIN      
        1 cgcaggcccg ccgcgaccgg ccgcgggagt cgtcatttcc cgcagtctgt actaggctgc
       61 gccgccgcag ggaaggagac gagccaccat gcaggcaacc gccgcggtgt tccgcaaagt
      121 ccatgagccg atgacgatcg aaacggtcga actcgacaag ccgcgccgcc gcgaagtgct
      181 ggtgcgcacg gtggccaccg gcgtctgcca cagcgacctg cacgtggtgg acggcaacgg
      241 ccgcttcccg ctcgaccgcc ccttcgtgct cggccatgaa ggcgccggcg tggtggaggc
      301 ggtcggcgac gatgtcaccg cggtgcgcaa gggcgaccac gtggtggcgt gcctgtccgg
      361 cttctgcggc aattgcccgc aatgcctggc cggccatccc aatctgtgca ccggcggcat
      421 cgtcgcccgc gcggaaagcg acacgccgcg catatcgcag ggtggccagc cgttccgcag
      481 cttcgcggcg atcggcagct acgcgagtca catgctgctg cacgagaact cgctggtgcg
      541 catcgatccg gacctgccgc tggaccgcgc ctgcctggtc ggctgcggcg tgatcaccgg
      601 cgtcggcgcg gcgttgcgtt cggccggatt gcaggcaggg cagacggtag cggtgttcgg
      661 ctgcggcggc gtcggcctgt cgatcatcca gggcgcgcgc atcggtggcg cacggcagat
      721 catcgcggtg gacatgttcg attccaagcg cgacatggcc atgcatttcg gcgccacgca
      781 tttcgtcaac agcagcgagg atgacccggt gaaggcggtg cgcgcgctga ccggcgggct
      841 gggcgtggac caggcgttcg aggccgtcgg catcagcaag ttgtgccggc aggcgatcga
      901 aagcctggcg atccgcggca ccgcgaccat cgtcggcgtg ctgccgccgg atgccatgat
      961 cgagttcccc tggatggcga tccgccccga atgccgcgtg cagacctcgc gcatggggtc
     1021 caaccagttc cgcagcgaca tcccgcatta cctggaattc taccgccagg gccggctgaa
     1081 cctggacgac atgatcagcc gccgcgcgcc gctggacggg gtgaacgagg cgttccgcgc
     1141 catgaaggcg ggcgaagtgg cccggaccgt gctgatgttc gactagccag ttccgccagg
     1201 ggggaaacac catgctgacc accacgcgtg acatgatcct gccgaccgcg atcaccggct
     1261 cctatccgcg gccgctgtgg ttcgatctca gcctgcacgg gcggtccttc aaatccgcgc
     1321 tgggcgagtc gctgttccgc gaacagtacc tggacgcggt cgccgcggtg atcaacgcgc
     1381 aggaagcggc ggggctggac atcgtcaccg acggcgacag caggttcgat ctcgcggtcg
     1441 gcggcaagtc ctggttcttc tatcccatcg aacggcttgg cggcatcgac gggcatcgcg
     1501 atacgtcgca aggctggatg ggccggcaca atctccggcc gggccatatt ctgtgggagg
     1561 tgcaggaagc ctaccagccc gccatcgtga aacagaagct gacgcgcggt gcctgggaat
     1621 acaccgcgct gtggcaggtg gcgcagcggc tcaccgacaa gccggtgaag tttggcgcga
     1681 tctgcgcacc ggccctcgcc agcatgctgt ggaacgaata ctacccggac aaccgggcga
     1741 tgatcctgga cctgtgcgac atcatgaacg cggagctgcg cgagatggcc gccgccggat
     1801 gtccgctgat ccaggtggag gaaccgccgc atcatggccg ctcgctgcgg ccggactgca
     1861 ccgaggagga attgcagttc ctgaccgacg cgttcaaccg ccagctcacc ggcgtggatg
     1921 cggagatctg ggtgcacacc tgctggggca atcccaacca gcagcggctg tactgggagg
     1981 cgccgagcta tgaacgtgcc atgccgtatc tgctgcaact ggatgcggac gtgatcacct
     2041 tcgaatgtgc cagcagcaac ggccgcgacc tgcatctgtt cggcaagtac cagaccgata
     2101 agaagatcgg cattggcgtg gtgaaccact gcaccaccgt ggtggaaccg cccagcctgg
     2161 ttgccgacct gatccgccgc gcgctggaat tcatcccgcc ggaacggctg gtcatcacca
     2221 ccgattgcgg cttcggccgc gagggactca gccggcgtat cgcgcattac aaatgcgtct
     2281 ccatggtgca ggggaccaac atcgtccgcc gcgaactcgg gctgcccgag gttcccatcc
     2341 gcgccgccga tccccggctg tggttcgccg gcaccaccga acactgacgg agacaaccga
     2401 tggccaaggg catcctgctg gtcgcgttcg acttcaccaa cgcgcatgag gacgaattcc
     2461 acgactggta cgacctggag catgtgccgg agcgtcagcg tgttcccggc ttcggcgcct
     2521 gcgaccgctg gatcagcatc agcaatccga agcaggcggt ggccagctac gagctggaca
     2581 gcatcgacgt gttgcaaagc ccggcgtacc gcgccatcgc cggcgacaac ctgtcggtgt
     2641 ggtcaaagcg cgtgacggcc atgtgcacgc gcctgctgcg gctggagggc gagcagacgc
     2701 tgcccggcaa ccaatccgct ccgcagggcg ccggcgggct gctggtgaac gcgatgagtg
     2761 tggatccgga atacgaggcg gaattcaacg agtggtatga tcacgagcac atcccggcgc
     2821 tgtccgcggt gcccggcacg ctgtgcgccc gccggttccg cgaccggcac ggctcgccgc
     2881 gctacctggc gctgtaccac ctggcctctc cggacgtgca ggccac
//
LOCUS       DASSZH010000425         2512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_708902, whole genome shotgun sequence.
ACCESSION   DASSZH010000425 DASSZH010000000
VERSION     DASSZH010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708902"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="VFN42_09155"
     CDS             complement(<1..968)
                     /locus_tag="VFN42_09155"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET8996823.1"
                     /translation="MRPAARMRVPMIPCFPGVDMAEQSRISRRRAHLTASVGMLTIGL
                     AVASGASARAASDPATQVGAEFANQSLPQNLPAADLARLYPQSWTTYAATAGRNPVFP
                     LPDTAPRDLRDGVRWSFAGAGALPLDGPPLAGDFKTTAYTVGMPVGVSVVAGIVFVGD
                     DNGYTYALNAETGKLIWAHYGWNMTMSNPLVSGDRVFVSTGNAYFNYANTMKYLHGER
                     PTRGPGLNTIYALDRGTGKEIWAFHPPGEAMPTPLLDGGFLYAGTGDGHVYKLSAETG
                     ALAWKADIVSFVSMSSLVKGDGRLFVGGTDPNFLYAIDEATGKVAW"
     gene            complement(1406..2125)
                     /locus_tag="VFN42_09160"
     CDS             complement(1406..2125)
                     /locus_tag="VFN42_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8996824.1"
                     /translation="MPRALVTGGSNGIGRAVVRMLLDAGYDVVSLDRAPSPLSHPTLS
                     TISVDLTDEAATAAAAGTALRGGPITTLIHNAGAIRPAMLADASTADLAVLSRLHLAA
                     PLLLARTMLPGMRAARFGRIVLVSSRAALGLATRSAYAATKAGMIALTRTWALELGPD
                     GITANAVAPGPIETDMFHAILPQGDPRIATLAQSIPVRRLGRAEDVARAVLFLADPAN
                     SFITGQTLFVCGGSSVGSLGL"
     gene            complement(2214..>2512)
                     /locus_tag="VFN42_09165"
     CDS             complement(2214..>2512)
                     /locus_tag="VFN42_09165"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HET8996825.1"
                     /translation="ACGHVQYPPRACCESCLSDSIVAETTESAAGTLLARTVLHHSQE
                     PAFRPQLPLGLGLVQLDAGPIVLCFMPEPCTIGTHVELIGTAAAAGLTARVIRG"
BASE COUNT          444 a          809 c          869 g          390 t
ORIGIN      
        1 ttccacgcca cctttccggt tgcctcgtcg attgcgtaca ggaagttcgg gtcggtgccg
       61 ccgacaaaca gtcgcccgtc gcccttcacc agggaggaca tgctcacgaa cgagacgatg
      121 tcggctttcc aggcgagcgc gccggtttcg gcggacagct tgtacacatg gccatcgccc
      181 gtcccggcat agagaaaccc gccgtcgagc agcggcgtcg gcatcgcctc ccccggcgga
      241 tggaaggccc agatttcctt gccggtgccg cggtcgaggg catagatcgt gttcagcccc
      301 ggtccccgcg tcgggcgctc gccgtgcagg tatttcatcg tgttggcata gttgaaatag
      361 gcgttgccgg tcgagacgaa caccctgtcg ccggagacga gcggattgct catcgtcatg
      421 ttccagccgt aatgcgccca gatcagcttg ccggtctcgg cgttgagcgc ataggtgtag
      481 ccgttgtcgt cgccaacgaa cacgatgcct gcgaccacgc tcacgcccac cggcatgccg
      541 accgtgtagg cggtggtctt gaaatcgcct gccagcggcg ggccatcgag cggcagcgcg
      601 ccggccccgg caaatgacca gcgtacgccg tcgcggagat cccgcggcgc ggtatcgggc
      661 agcggaaaca cgggattgcg acctgccgtc gcggcgtacg tcgtccagct ttgcgggtag
      721 agcctggcca aatcggccgc cggcagattt tgcggcagcg attgattggc gaattccgcg
      781 ccgacctgcg tcgccggatc agatgcggcg cgggcgctgg cgccggacgc aacggcgaga
      841 ccaatggtca gcatgccaac gctggcggtc agatgcgccc ggcggcgcga gatgcgtgac
      901 tgttcagcca tgtcaactcc aggaaagcag gggatcatcg gaacgcgcat ccgggctgcg
      961 ggacgcatgt cctggtcagg accgcgtcga taagcgccca gggtgccgcc gtgtcgcctc
     1021 cgaatgtctc acgacattac ctccaggcgc ggaacgatgt tgtgggagac cacggcgcgc
     1081 cggacttgca cggcgatcgc cgcgcgaggc aacataataa aaatttatag cattttattg
     1141 caactataat tcggaatata tccggcgcag cgcgggcgaa gccgggagcg gcaaaggggg
     1201 cgagccacgg atgcccggcg gcgcgcgacg ccaggccgtt ctcgatcgcg acataggccg
     1261 gccacaagtt tcgtagcagc aacgcatagc cggccggtcc gcagcgtccg tgaacaagct
     1321 cctgcacgat gccgccaacg atgcggtcgc gtcccgcaat gccgcggcaa ggccgggcgc
     1381 gggcatgtcg atgatgtctg cggcgctaca ggccgagact gccgacgctg ctgccgccgc
     1441 agacgaacag cgtctggccg gtgatgaagg aattggcagg gtcggcgagg aacagcaccg
     1501 cgcgtgccac gtcctccgcc cggccgagcc gtcgcactgg gatgctctgc gcgagggtcg
     1561 cgatgcgcgg atcgccttgc ggcaggatcg catggaacat gtcggtttcg atcgggccgg
     1621 gagcaactgc gttcgcggtg atgccgtccg gcccgagttc caacgcccag gtgcgggtca
     1681 gcgcgatcat gcccgccttg gtcgccgcat aggcgctgcg cgtcgcaagg ccgagtgcgg
     1741 cgcgcgagga taccagcacg atgcggccaa agcgcgccgc gcgcatgccc ggaagcatcg
     1801 tccgcgccag cagcagcggg gccgcgagat gcaggcggga aaggacggcg agatcggcgg
     1861 tgctggcatc ggccagcatt gccgggcgga tggccccggc attgtggatc agcgtcgtga
     1921 tcggtcctcc ccgcagcgcc gttcctgccg cggccgcggt cgcggcctca tcggtcaggt
     1981 ccacgctgat cgtggacagc gtgggatgcg acagcggaga gggggcgcgg tcgagcgaga
     2041 ccacgtcgta gccggcatcc agcagcatgc gcacgacggc acggccgatc ccgttgctgc
     2101 cgccggtgac gagggcgcga ggcatcacgc ggcgttgaaa acgtgagtgc agtcaccgga
     2161 taaaacggat tccgcagaaa ccaaaaaggc aaacctcatc tgcgcaggct gcgtcatccg
     2221 cggatcaccc tcgctgtcag tcccgccgcc gcggcggtgc caatgagttc gacatgcgtg
     2281 ccgatcgtgc agggttcggg catgaagcac agaacgatcg gtcccgcgtc cagttgcaca
     2341 agtcccagac caagcggcag ttgcgggcgg aacgccggct cctggctgtg atgcagcacg
     2401 gtgcgcgcca gcagcgtgcc ggccgccgac tccgtggttt cggcgacgat gctgtcggac
     2461 aggcatgatt cgcagcacgc gcgcggcgga tactggacat gtccgcacgc cg
//
LOCUS       DASSZH010000426         2187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_862287, whole genome shotgun sequence.
ACCESSION   DASSZH010000426 DASSZH010000000
VERSION     DASSZH010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862287"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1604
                     /gene="gpmI"
                     /locus_tag="VFN42_09170"
     CDS             75..1604
                     /gene="gpmI"
                     /locus_tag="VFN42_09170"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HET8996826.1"
                     /translation="MPNAAPRPVMLVILDGFGWRAERADNAVAQAAKPNFDRLWQACP
                     HAFLRTSGRDVGLPDGQMGNSEVGHMNIGAGRVVHQELVRIGDAVADGSLAQAAPLND
                     FIAKLRQSKGTCHLLGLVSPGGVHSHQDHAAALGRILDDAGIPTVLHAFTDGRDTPPE
                     SGAAFVATLRDALPAAVPIATVIGRYFAMDRDKRWDRVAQAYQAMAEAQGARHPTPAA
                     AIEAAYAAKKFDEFVPPAVIGNYAGMNDGDAILCFNFRADRVREILGALLDPAFDGFP
                     RPRVLKFAAAVGMTRYSDALAPFMDVLFEPDTLKNILGEVVSAAGKTQLRMAETEKYP
                     HVTYFLNGGRETPYPGEDRIMVPSPKVATYDLQPEMSAPELTDKAVAAIESGKYDLIV
                     LNYANPDMVGHTGSLPAAIKAVETVDTGLGRIARAIRNQNGALLVTADHGNCELMKDP
                     ETGGPHTAHTTNPVPVVLMGTNAPSIHEGRLADLAPTLLALLGIPQPPEMTGRSLIGT
                     T"
     gene            1606..2076
                     /locus_tag="VFN42_09175"
     CDS             1606..2076
                     /locus_tag="VFN42_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET8996827.1"
                     /translation="MTNRPPLPPFTRDTAEQKVRSAEDGWNTCNPERVSLAYTQDSVW
                     RNRNEFLHGRPAIVAFLARKWNRELDYRLIKELWSFTDNRIAVRFAYEYRDDSNTWFR
                     AYGNENWEFDPHGLMRVRHASINDLPITPDARLFHWDRAMPRPAGHPGLSELGL"
BASE COUNT          373 a          766 c          692 g          356 t
ORIGIN      
        1 ggccgtaagc ccgacattgt tgcggcgcgg cggacgggtt acgtctgtcc tgtgccgccg
       61 aaggatgcgc ctccatgcca aacgccgcgc cgcgtcccgt gatgctggtc attctcgacg
      121 gtttcggctg gcgggcggag cgcgccgaca acgccgtggc gcaggccgcc aagccgaatt
      181 tcgaccggct gtggcaagcc tgcccgcatg cgttcctgcg cacctccggc cgcgatgtcg
      241 gcctgccgga cgggcagatg ggcaattccg aagtcggcca catgaacatc ggcgccggcc
      301 gcgtggtgca ccaggaactg gtgcgcatcg gcgacgccgt cgccgacggc agcctggcgc
      361 aggccgcacc gctgaacgac ttcatcgcga aactccgcca gtcgaaaggc acctgccatc
      421 tgctcggcct ggtgtcgccc ggcggggtgc attcgcacca ggaccatgct gccgcgctgg
      481 ggaggattct ggatgatgcc gggatcccaa ccgtgctgca cgccttcacc gacggccgcg
      541 acaccccgcc cgaatccggc gccgccttcg tggcgaccct gcgcgacgcc ctgcccgcgg
      601 cagtgccgat cgccaccgtc atcggccgct atttcgccat ggaccgcgac aagcgctggg
      661 accgcgtcgc gcaggcctat caggcgatgg ccgaagccca gggcgcgcgc catccgacgc
      721 cggcggctgc cattgaagcg gcgtacgccg caaagaagtt tgatgaattc gtcccgcccg
      781 cggttattgg caactacgca gggatgaacg acggtgacgc catactgtgc ttcaacttcc
      841 gcgccgaccg cgtgcgcgaa atcctcggcg cgctgctcga tccggcgttt gatggcttcc
      901 cgcgcccacg tgtgctgaaa ttcgccgccg ccgtcggcat gacgcgctat tccgatgcac
      961 tggccccgtt catggacgtg ttgttcgagc cggacacgct gaagaacatc ctgggggagg
     1021 tcgtctcggc cgccggcaag acacaactgc gcatggcgga gaccgaaaag tatccccacg
     1081 ttacctactt cctgaatggc ggccgggaaa cgccctatcc tggggaggat cggatcatgg
     1141 tcccatcgcc gaaggtcgcc acgtatgacc tgcagcccga gatgtccgcc ccggaactga
     1201 ccgacaaggc ggttgcggcg attgaaagcg gcaagtacga cctgattgtg ttgaattacg
     1261 cgaaccctga catggtcggc cacaccggca gcctgccggc ggcgatcaag gccgtggaga
     1321 cggtggatac cggccttggc cgcatcgcca gggcgatccg caatcagaac ggcgcgctgc
     1381 tggtcaccgc cgatcacggc aattgcgaac tgatgaagga ccccgaaacc ggcggcccgc
     1441 acaccgcgca caccaccaac cctgtccccg tggtgctgat ggggaccaac gccccgtcta
     1501 tccacgaagg ccgtcttgcc gatctcgcgc cgacgctgct ggccctgctg ggcatcccgc
     1561 agccgccgga gatgaccggc cgttcgctga tcggaaccac ctgacatgac gaatcgcccg
     1621 ccgctgccgc ccttcacccg cgataccgcc gagcagaagg tccgctcggc ggaggatggc
     1681 tggaacacat gcaaccccga acgcgtgtcg ctggcctaca cccaggacag cgtctggcgg
     1741 aatcgcaacg aattcctcca tggccgtcct gcgatcgtcg ccttcctggc ccgcaagtgg
     1801 aatcgcgagc tcgactaccg gctgatcaag gaactctggt ccttcaccga caaccgtatc
     1861 gccgtgcgct tcgcctatga gtaccgcgac gactcgaaca cctggttccg ggcatacggc
     1921 aatgagaact gggaattcga cccgcacggg ctgatgcgcg tccggcatgc cagcattaat
     1981 gacctgccga tcaccccgga tgcacgcctg ttccactggg atcgtgcaat gccgcggccg
     2041 gccgggcatc cgggcctgtc cgagctggga ttgtagcttc gggcgtcttg tcggaccgtg
     2101 cggcagcctc acatcaccgt catcgcgtcg cacccggccg ctgctggtgg ccgccgggcg
     2161 taccttggtc ctgcggccgg ttgccgg
//
LOCUS       DASSZH010000427         6475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_299932, whole genome shotgun sequence.
ACCESSION   DASSZH010000427 DASSZH010000000
VERSION     DASSZH010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6475
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299932"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..383)
                     /locus_tag="VFN42_09180"
     CDS             complement(78..383)
                     /locus_tag="VFN42_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET8996828.1"
                     /translation="MAQQRRNLAPEAQAAFDAFGKAVFADGALSAKMKQIIAVAVAHV
                     TQCPYCIKGHTKAARRAGATPQELMEAIWVAAEMRAGAAYAHAALMLDTLESETGER"
     gene            complement(616..1875)
                     /locus_tag="VFN42_09185"
     CDS             complement(616..1875)
                     /locus_tag="VFN42_09185"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HET8996829.1"
                     /translation="MTAIANRLLPVQGAKAPGGSAKAAIPRYRATRAPALLSAGFRPF
                     FLLSAVWACIGIPLWLALYGGTVTLPTALPPADWHAHEMIFGFAAATVAGFLLTAIPN
                     WTGRMPLQGWPLATLVLLWAAGRIGVLFSAVLGPAAAAVLDLLFPAAFLAVVTREILT
                     GRNWRNLPMVGALTGLLLANALVHAAALGLADTAEFGNRFAIAILLLLISLVGGRIIP
                     SFTRNWLVKQRPTVPAPVPANRFDTVVLSVTAVALAAWLLAPGTLETALIGLAAGLAH
                     ALRLSRWRGLATLREPLLWVLHLGYAWLAAGFCLLGLQIVVPWLPPFTALHALTVGAI
                     GTMTLAVMTRATLGHTGRPLQASTGTTAIYLLVTVAALLRLLAPFAPAHALPALWLAG
                     LAWSAAFGLFVLLYLPLLAQPRQTGKA"
     gene            complement(1897..2157)
                     /locus_tag="VFN42_09190"
     CDS             complement(1897..2157)
                     /locus_tag="VFN42_09190"
                     /inference="COORDINATES: protein motif:HMM:NF040453.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6522 family protein"
                     /protein_id="HET8996830.1"
                     /translation="MTSSAIDGAAASAIADPPVPLDAGIIAPMLGLSPETFMRGVQRG
                     MIVQRTERGLDEDAGWFRVTFRFRRRRCRIRFNPDTGTIVPA"
     gene            2468..3187
                     /locus_tag="VFN42_09195"
     CDS             2468..3187
                     /locus_tag="VFN42_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET8996831.1"
                     /translation="MHASIDSAMLARAPLFAGMPAGGLSEVASAASAVRCMPGETLFG
                     QDDPADAFFVLISGRVKIAQVTVEGHQVVLRYIGPGEVFGAVPLFTNTGYPAASIAVT
                     DSMALRWDRATTHRLLQRYPSIVANALTIVGRRLHELQNRYRELATERVEQRVGRAIL
                     RLLEKAGHRERSGVQIAFPLSRQDIAEMTGTTLHTVSRILSEWEKRGVVQGGRMRVII
                     RDPAALARIADDLPPGLTGEA"
     gene            complement(3243..3722)
                     /locus_tag="VFN42_09200"
     CDS             complement(3243..3722)
                     /locus_tag="VFN42_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996832.1"
                     /translation="MQGIAIALALHVLAIVLWIGGVGFVTLALFPALRGIPDPRQRLM
                     LFETLEHRFSLVARASILLAGATGLYLLFRLDAWSWFAHAAFWWLDAMVLVWLLFALM
                     LFVLERLLLHRRFRQWAEASPDTAFAAALWFHRLMLALSILTILGAVAGSHGASLFG"
     gene            complement(3760..4212)
                     /locus_tag="VFN42_09205"
     CDS             complement(3760..4212)
                     /locus_tag="VFN42_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HET8996833.1"
                     /translation="MPVTFSRPANPKAPGTAVGIDEPLIRSLVERFYGRVRRDPLLGP
                     IFEAAVTDWPEHFDKLTDFWSSLTLMSGRYKGNPFEAHLPLPRLGDEHFATWLLLFDQ
                     TLHELCTPEQAEVFRLRAGRVAESLNMGLAIRRGEAPLPARRLCPAGQ"
     gene            4667..5176
                     /locus_tag="VFN42_09210"
     CDS             4667..5176
                     /locus_tag="VFN42_09210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET8996834.1"
                     /translation="MRLTAFTDYTLRVLMYLALNQDRTATVAEIADAYGISRLHLNKV
                     VLELAQAGDIETTRGRNGGIRLAKRPDAINLADVVRRAEPDMQLAACFGSARACVLGR
                     CCLLQGVLQRASHAFFTELARHTLADLVGTGGPTAMVFLNQQPPERPAPRRRLPPESS
                     GRNRSASSR"
     gene            complement(5257..5344)
                     /locus_tag="VFN42_09215"
     tRNA            complement(5257..5344)
                     /locus_tag="VFN42_09215"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5308..5310),aa:Ser,seq:tga)
     gene            5456..6445
                     /locus_tag="VFN42_09220"
     CDS             5456..6445
                     /locus_tag="VFN42_09220"
                     /inference="COORDINATES: protein motif:HMM:NF021497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HET8996835.1"
                     /translation="MATRTTTALVLAALLPVAAQAAPPPDPAAILTLQGENASISAGN
                     PTDRLYTNGLRLEWTSPTGQVPGFLASFGQALWGAGQQRIAVGLAQQIYTPLDTSVAP
                     ADPHDRPYAGYLATNISLLSDTDTTRSTLTLSLGVVGPASGAEDLQNGFHDLIGQAHD
                     RGWGSQIPNTFAFELLGGRTWRIPTGRFGSLETDVLPAATIGVGDLRDYLQAGVTLRL
                     GQGLNSDFGVPRITPGLSGGDAYTPTRPFVWYVFAGVDGQAVAYDLLLQASPFRSGPH
                     VDPVWDVGEFQAGAAIIAYGVRVTVAYVMRTQEFAGQGGGLHQFGSASVGLRF"
BASE COUNT         1142 a         2193 c         2140 g         1000 t
ORIGIN      
        1 gggtggatcg cgcctcgcgg gactcgttcc ccgccagcga tcctccaggc tggatctggc
       61 gctgagccca tcggtattca gcgctcgccg gtctcgcttt ccagcgtatc caacatcagc
      121 gcggcgtgcg cataggccgc accggcacgc atttcagccg cgacccagat ggcctccatc
      181 agctcctgcg gcgtggctcc ggcgcggcgt gccgctttgg tgtgaccctt gatgcagtag
      241 gggcactgtg tgacgtgtgc gacggccacc gcgatgatct gcttcatctt cgccgacaag
      301 gcaccatcgg caaacacggc cttgccgaag gcatcgaatg ccgcctgggc ttctggtgcc
      361 aggttgcggc gctgctgcgc catgtcgcgg gttgccggag ggtacattct gtctggcatt
      421 cctttctcct gtgattatcg ccgtcattgg cctggccggc cggttgcttg gaacggacca
      481 gcgttcagcg gggcagcgca acccgcctgc gcgtcagcgg gtatcctggc ataatatcgt
      541 tatacgatac aacgccgcag atggtcagtc acgctggccg ttgacacgaa acaggcgacg
      601 cttgaccgat tggcgtcagg ctttccccgt ctggcgtggc tgcgccagca acggcaggta
      661 gagcagcaca aacagcccga atgcggcgct ccacgccagc ccggccagcc acaacgccgg
      721 cagggcgtgt gccggggcga agggcgccaa cagccgcaac agcgccgcga ctgtcaccag
      781 gaggtagatt gccgtcgtgc cggtgctggc ttgcagcggc cggccggtat ggccgagcgt
      841 ggcgcgggtc atcacggcca gggtcatggt gccgatggcg cccacggtca gtgcatgcag
      901 ggccgtgaag ggcggcagcc agggcaccac gatctgcagc ccgagcaggc agaacccggc
      961 cgccagccag gcatacccca ggtgcagcac ccaaagcagc ggttcgcgca gcgtggccag
     1021 gccgcgccag cggctcaacc gcagcgcatg cgccaggcca gcggccagac caatcagcgc
     1081 ggtttccagc gtcccgggcg caagtagcca tgctgcgagc gcaaccgcgg tgacgctcag
     1141 cacaacggtg tcgaaccggt tggccggcac cggcgccgga acagtggggc gctgtttgac
     1201 cagccagttg cgggtgaagc tgggaatgat gcggccgcca accaagctga tcagcagcag
     1261 caggatggcg atggcgaacc ggttgccgaa ttccgcggtg tcggcaagcc ccagcgcggc
     1321 ggcatgcacc agggcgttgg ccagcagcag acccgtcagc gcccccacca tcggcaggtt
     1381 gcgccagttg cgcccggtca ggatctcgcg cgtcaccaca gccaggaagg cggcgggaaa
     1441 cagcagatcg agcaccgccg ccgcggcagg accgagcacc gccgagaaca acacaccgat
     1501 gcgtcccgca gcccagagca agaccaaggt tgccaagggc cagccttgca gcggcatgcg
     1561 gccggtccag ttgggaatcg cggtgagcag gaatccggcc acggtggccg cggcgaagcc
     1621 gaagatcatc tcgtgcgcat gccagtccgc cggcggcagc gcggtcggca gcgtcaccgt
     1681 gccaccatac agcgccagcc acagcggaat gccgatgcag gcccataccg cggatagcag
     1741 gaagaacggc cggaacccgg cggacagtag ggcgggtgcc ctggttgccc gataacgcgg
     1801 gattgcggcc ttggcagaac cgcccggcgc tttcgcgccc tggacaggca ggagtcgatt
     1861 ggcgatcgcg gtcatgtctg gctcaccgca gcaggttcag gcaggcacga tcgtaccggt
     1921 atccgggttg aaccggatcc ggcaacgacg cctgcgaaaa cgaaatgtca cgcggaacca
     1981 gcctgcgtct tcgtccagac cgcgctcggt gcgctgcacg atcatgccgc gctgcacgcc
     2041 gcgcatgaat gtctccggcg atagtcccag catgggcgct atgatgcccg cgtcgagcgg
     2101 cacgggcgga tcggcaatcg cgcttgctgc ggcgccgtcg atggcggaac tggtcatgcc
     2161 ggtctcctgt cgtcctggaa atcctgtagc ggcccttcac gccggcgtct ttgcgctgcg
     2221 acaaacagcg acgggccacg gcgctctgac agagatcaag ccgaaccgga ttgcctgctt
     2281 gagcatcgcg gaggctccct gcgaccggta tcgggtatcc cgccgatgcc gtctcgccgc
     2341 gtgccctggg cgcttgttgg tgccaaactt gtccggaaag gcgatggggg atggcgacag
     2401 cgacctgctg gtgttaacgc cgaacccgca ctgtggctgc cggacgcagc cagcacagga
     2461 gggcgagatg catgcctcga tcgacagtgc catgctggcc cgcgccccgc tgtttgcagg
     2521 aatgccagcc gggggattga gcgaagtcgc gtccgccgca tccgcggtcc gttgtatgcc
     2581 gggcgaaacg ctgttcgggc aggacgatcc ggctgacgcg ttcttcgtgc tcatcagcgg
     2641 ccgggtgaag atcgcgcagg tgacggtgga aggacatcag gtggtgctac gctatatcgg
     2701 cccgggcgag gtgttcggcg ccgttccgct gttcaccaac accggctatc cggccgcctc
     2761 gatcgccgtt accgacagca tggcgctgcg ctgggaccgg gcaaccaccc accgcctgct
     2821 gcagcggtat ccatccattg tggcgaacgc gctcaccatc gtcgggcgcc ggctgcacga
     2881 gttgcagaac cgctatcgcg aactggcgac cgagcgtgtg gaacaacggg taggccgggc
     2941 cattctgcgc ctgctggaga aagccggtca tcgcgagaga tccggcgtac agatcgcgtt
     3001 tccactgtcc cgccaggata ttgccgagat gaccggcacc acgctgcaca ccgtgagccg
     3061 catcctcagc gagtgggaaa agcggggcgt cgtccagggt gggcgcatgc gggtgatcat
     3121 ccgcgacccg gccgcactgg cgaggattgc cgatgacctg ccgcccggtc tcaccggcga
     3181 ggcgtgaccc gatgcgcggc ctgcaggttc accgaaagcc gtagctgccg tgccgaactg
     3241 gcttatccga acagggaagc gccgtggctg ccggcaacgg cgccgaggat ggtcaggatg
     3301 ctcaaggcca gcatgaggcg gtggaaccac agcgccgcgg caaatgcggt gtccggcgag
     3361 gcttcggccc attgacggaa gcggcggtgc agcagcagcc gctccagcac gaacagcatc
     3421 agcgcgaaca gcagccagac cagcaccatg gcgtccagcc accagaacgc ggcatgggca
     3481 aaccaggacc aggcgtccag gcggaacagc aggtagagcc ccgtggcgcc ggcaagcaga
     3541 atgctggcgc gggcgaccag ggaaaatcgg tgctccaagg tctcgaacag catcaggcgc
     3601 tgccggggat ccggtattcc gcgcaaggcg gggaacaagg ccagagtgac gaagccgacg
     3661 ccgccgatcc acaacacgat ggccagcaca tgcagagcca atgcgattgc gatgccctgc
     3721 atggttctgc tctcacctca cgaatggcga acgatccagt tactgacctg cgggacatag
     3781 gcggcgagcg ggaagcggcg cttccccccg gcggattgcc aggcccatgt tcaggctttc
     3841 cgccacgcgg ccggcccgca ggcggaaaac ctcggcctgt tccggcgtgc acagttcatg
     3901 cagggtctgg tcgaacagaa gcagccaggt ggcgaagtgc tcatcaccca ggcgcggcag
     3961 cggcaaatgc gcctcgaacg gattgccttt gtagcggccg ctcatcaagg tcagcgacga
     4021 ccagaagtcg gtgagcttgt cgaagtgctc cggccagtcg gtgaccgcgg cctcgaagat
     4081 cggtccaagc agcggatcgc ggcgaacccg gccatagaac ctctccacca gggagcggat
     4141 cagcggctca tcgatgccca ccgccgtgcc gggagctttc ggattggcgg ggcgggagaa
     4201 agtcacaggc atcgagctgt tccgtctggc ttgcatcggg gcagggggcc tacgcggcgc
     4261 atcctggtca tctccaagct agcccgggcg gcgataaaaa acaagatacg tgataccact
     4321 tatgggccaa ccaccgtcca gcggcgcggc ggcggatacg gacgtgatgc tcctgcttgc
     4381 cgcgttctcg tccgcccgct gcgcggcggc ggacgcaaag actgtgaccg cggatcgaca
     4441 ttatcggata aatgtgccaa tatcaatacc gattataggc gtgcatgcga gggcagacct
     4501 ggctttacac agggctcaag ccgcgaggct ctgcagtaga actggtactt gtgatccgaa
     4561 ttctggcgac ctatcatgct gaccggccta agggcggctc gtttgccgcc gtggtcccgc
     4621 cgtgcccgtc gcgccgcctg cttcacctgg acatcatcgc cgaaccatgc gcctgaccgc
     4681 tttcaccgat tacacgctgc gggtgctgat gtacctcgcg ctgaaccagg atcggaccgc
     4741 gaccgtcgcc gagatcgccg acgcctacgg catttcccgg ctgcatctga acaaggtggt
     4801 gctggaattg gcgcaggccg gagacatcga gacgacgcgc ggccgcaatg gcgggatccg
     4861 gctggccaaa cggccggacg cgatcaacct tgccgacgtg gtccgccgag ccgaaccgga
     4921 catgcagctg gcagcctgct tcggcagcgc ccgtgcctgc gtgcttggac gctgctgcct
     4981 gttgcagggc gtgctccagc gtgcgagcca cgcattcttc accgaactgg ctcgccacac
     5041 cctggctgat ctggtgggca cgggcgggcc gaccgcgatg gtgttcctta accagcagcc
     5101 gccagaacgg ccagccccgc gccggcggct gccgccggaa tcgagcggaa ggaatcgcag
     5161 cgcctcaagc cgctgagcga gtgcgtccgc agctttcagt gcagcatccg ggatgtcatc
     5221 ccgaaacccg agctttgggc ggaggaaatg ccgctctggc ggatggggtg ggattcgaac
     5281 ccacgaaccc ttgcgggttg gcggttttca agaccgctgc cttaagccgc tcggccaccc
     5341 atcctgcaga tcgcaccccg ccatatcata cctattgacg aaccggcgag cggtctgtct
     5401 cctgccagcg taacaaccac ccactcgcac gcacgggtcc ggagtcacgc gcctgatggc
     5461 caccagaaca acaacggctc tcgtcctggc cgccctgctg ccggtcgctg cacaggcggc
     5521 ccctccgccc gatcctgccg ctatcctgac gcttcaaggc gagaatgcct ccatctccgc
     5581 cggcaatcca accgatcgcc tctacaccaa cggcctgcgg ctggaatgga cctcccccac
     5641 cggccaggtg cccggcttcc tcgccagttt cggccaagcg ctgtggggcg cgggccagca
     5701 gcgcatcgcc gtcggcctgg cgcagcagat ctacaccccg ctggacacca gcgtggcgcc
     5761 ggccgacccg catgaccggc cctatgccgg ctatctcgcc accaacatct cgctgctcag
     5821 cgacaccgac accacccgca gcacgctgac gctcagcctc ggcgtggtcg gccccgcctc
     5881 cggcgccgag gacctgcaga acggcttcca cgacctgatc ggccaggcgc atgaccgcgg
     5941 ctggggttcg cagatcccca acaccttcgc cttcgaactg ctcggcggcc gcacgtggcg
     6001 cataccaacg ggccggttcg gcagcctgga gaccgacgtg ctgcccgccg ccacgatcgg
     6061 cgtcggcgac ctgcgcgact acctgcaggc cggcgtgacg ctgcgcctcg gccaggggct
     6121 gaattccgat ttcggcgtgc cgcgcatcac acccggcctg agtggcggcg acgcctatac
     6181 gcccacccgg cccttcgtct ggtacgtgtt cgccggcgtg gacggccagg cggtggccta
     6241 cgacctgctg ctgcaggcca gcccgttccg ctccggcccg catgtcgatc cggtgtggga
     6301 tgtgggcgag ttccaggccg gcgccgccat catcgcctac ggcgtgcggg tgacggtcgc
     6361 ctatgtgatg cggacccagg aattcgccgg ccagggcggc gggctgcacc agttcgggtc
     6421 cgcctcggtc ggcctgcggt tctagacccg catcatccgc cccggttcgc cccca
//
LOCUS       DASSZH010000428         7138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_487267, whole genome shotgun sequence.
ACCESSION   DASSZH010000428 DASSZH010000000
VERSION     DASSZH010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7138
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487267"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VFN42_09225"
     CDS             <1..489
                     /locus_tag="VFN42_09225"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET8996836.1"
                     /translation="WICLPRLYLGIHYPSDLVFGALLGVGTVWGVAQAMEMRGGAPGH
                     WLMARIDAAERRAPQAFYAVGFLVSFEITMIFNDVRDLVRAVLHAMRALGFAAGEGVA
                     LFVTGGVLLLAAAALAVLRRSRSRRGGPRARGSRRINPRETPGLRAAALGHKERRFHP
                     SR"
     gene            complement(474..1583)
                     /locus_tag="VFN42_09230"
     CDS             complement(474..1583)
                     /locus_tag="VFN42_09230"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8996837.1"
                     /translation="MNVQVTSRPTPAAGNTSKLAIADGDIHPQRNSIRDIFPWMEKRW
                     QEHALTFGNRPRQAYQAGPAYPKGQPNASRRDAWPPGGKPGSNLEFMQRQLLDANNIT
                     LGILNATGDNGSSFQHREFGAAVCHAMNEWLVEHWLAPEPRLKGSIVVPFEDPEAAAR
                     EVERWAGDPRFVQVLMVTRTAEPPGQKRYWPLYEAANAANLPIGVHAFGFGGYPVSGA
                     GWPSYYIEDMVGHAQSAQAFLTSLVIEGVFERLPDFRLVLIESGFAWLPPLAWRLDKI
                     WRRLRMETPHLKRAPSEYIRERVWLTTQPMEEPQPREQVLDAIDWIGWDRLIFATDYP
                     HWDYDDPTQVLPMPLPEQRRRDFFFNNARALYREG"
     gene            complement(1580..2650)
                     /locus_tag="VFN42_09235"
     CDS             complement(1580..2650)
                     /locus_tag="VFN42_09235"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8996838.1"
                     /translation="MSVRATGAIDCDLHPAVPNLKALHPYLSDHWRDIVIQRGVHELD
                     SIAYPANSPLTARPDWRVDGRKPGADLDRLRRHALDPFGTAIGICNCLYGAQLLFSED
                     MGAGFARAINDWMAAEWLDREPRLRASIVVPAQNPELAVDEIERLAPDPRFVQVLLLV
                     MGDMPLGKRHYWPIYAAAQRHNLPIGIHAGSAYRHPVTPLGWPTYYTEDYAAQAAGFQ
                     QCLTSLICEGVFTKFPDLKVVLIESGFTWLPAHLWRLTKFWRGLRMEIPWVDRAPIDI
                     VRSNVRLTTQPCDAPPDAESFGKLLDHMGSDELLLFSTDYPHWQFDDQDALPDGLSPD
                     LVRKIMIDNPRATYARLTETVQ"
     gene            2777..3028
                     /locus_tag="VFN42_09240"
     CDS             2777..3028
                     /locus_tag="VFN42_09240"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HET8996839.1"
                     /translation="MSTHSVAEAKNHLPKLIDRALAGEAVLITRHGQPVVELKAIPVQ
                     PRRVSPEDLEWLARRRKDLRVPVEDLGALVSRMRDEDWG"
     gene            3025..3450
                     /locus_tag="VFN42_09245"
     CDS             3025..3450
                     /locus_tag="VFN42_09245"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HET8996840.1"
                     /translation="MSVYLDASFIVPLFANQEPLSRRAAVFLGDCLPELVISDFSGAE
                     FASAIARLTRMNALTTDQAEAIFADFDIWTRRATTNIETIGADIRAAAAYLRQLRLNL
                     RAPDALNIAVAQRVGATLATFDDRMAENAVRLGVEVAAV"
     gene            complement(3496..3903)
                     /gene="rbfA"
                     /locus_tag="VFN42_09250"
     CDS             complement(3496..3903)
                     /gene="rbfA"
                     /locus_tag="VFN42_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET8996841.1"
                     /translation="MLRDGDGAGLKKRDAGPSQRQLRVAEEIRHVLAGLFERRDFRDP
                     ELADAVITVTEVRTSPDLKHAIVFVSRLGRTDVDALLPALKRASVYLRGQVAHALRLK
                     FAPQLSFQPDHALDYAMKIDALMHRPEVARDLD"
     gene            complement(3873..6539)
                     /gene="infB"
                     /locus_tag="VFN42_09255"
     CDS             complement(3873..6539)
                     /gene="infB"
                     /locus_tag="VFN42_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET8996842.1"
                     /translation="MSGTNDQDTGKGGRLSLRPAGRLELGRTVDAGSVRQSFSHGRSK
                     VVQVEVRKKRAGAPAPAAPVQSGGGPAPARGPSQGGRGQQAPTRALTQTEQAARQRAL
                     VEQQREAARREAERREQEKISILSAAEEARRREEEARKAAEEEARRKEEEEAQQKAEE
                     EARKVAEATASAQAAAAKSTAPAARAPAQRAPEEPRRAPTQAPQRPGATPSETLRLRP
                     GTRTGEDEDEARPARRPGAGAPPRKPPVVAPKKAGEDRRRPGRIDVQAAIEGEDERVR
                     SLASVRRQRERERRQAELERLRSDQVKVVRDVILPDNITVQELANRMAARGGDVIKAL
                     MKLGVMATITQTLDADTAELVVQEFGHRARRVSESDVELGLEGGLDTESELVARPPVV
                     TIMGHVDHGKTSLLDALRSTDVAAGEAGGITQHIGAYQVTLESGEKITFIDTPGHEAF
                     TAMRARGASVTDIVVLVVAADDGVMPQTIEAIRHAKAANAPIIVAINKMDRPDANPTR
                     VRQELLQYEVVVEDMGGETQDVEVSALKKTGLDKLQEAILLQAEILDLKANPHRVAEG
                     SVIESRLDRGRGPVATILVQKGTLHTGDILVAGAEWGRVRAMLDDKARALKDAEPSTP
                     VEVLGLSGVPTAGEPFVVVDSESRAREITEFRARKLREKAATASAGARGTLDQMLARI
                     QAGEQKEVAVLIKADVQGSAEAIQATVLKLAHEEVKVRVLIAGVGQITESDIQLAKAS
                     DAVIVAFNVRATAQARELAQREGVDIRYYAIIYEVADDIEKLVRGKVAPKAREKFLGY
                     AEIRKVFTITKTGKVAGCMVTEGLVKRGAGVRLLRDNVVIHTGELSQLKRFKDDVREV
                     ARGYECGLSFANFHDLQEGDMVECFETEMVPA"
     gene            complement(6543..>7138)
                     /locus_tag="VFN42_09260"
     CDS             complement(6543..>7138)
                     /locus_tag="VFN42_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019085199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET8996843.1"
                     /translation="LTRERLPKERMIRFVVGPDRQVVPDLTARLPGRGLWLSAAGDVI
                     ETASAKGNLTRAFARAARGPVVVPPDLLAVLQAALIRRIGELLGLSRRAGQVVAGFDK
                     AREWVRNGRARLVVQASDGSEPERARLLSGATLPVFAPLPGAALGRVFGRDYVVHVAI
                     APGKLAESLCVEAERLAGLRRTGQNAAQRNEATGANG"
BASE COUNT         1034 a         2447 c         2409 g         1248 t
ORIGIN      
        1 tggatctgcc tgccgcggct ttatctgggg atccactatc cgtctgacct cgtattcggc
       61 gcgctgctgg gagtggggac ggtctggggg gttgcccagg caatggagat gcgcggcggc
      121 gcgcccgggc attggctgat ggcgcggatc gacgcggcgg agcggcgcgc gccgcaggcg
      181 ttctatgcag tggggtttct ggtttcgttt gaaatcacca tgatcttcaa cgacgtgcgc
      241 gacctggtac gggccgtgct gcatgcgatg cgcgcgttgg gtttcgcggc gggggagggg
      301 gtcgcgctgt tcgtgaccgg cggcgtgctg cttctggcgg cggcggccct ggccgtgctg
      361 cgccggtcac gcagccgccg cggtggcccg cgcgcccgcg gctcgcggcg catcaacccc
      421 cgcgagacgc cagggctgcg cgccgcggcg cttggtcaca aggagcggcg gtttcacccc
      481 tcgcgataga gtgcccgcgc gttgttgaag aagaagtcgc gccgccgctg ctccggcagc
      541 ggcatcggca gcacctgggt gggatcgtcg tagtcccagt gcgggtaatc ggtggcgaag
      601 atcagccggt cccagccgat ccagtcgatc gcgtccagca cctgctcgcg cggctgcggc
      661 tcctccatcg gctgcgtggt cagccacacg cgctcgcgga tgtactcgga cggcgcgcgc
      721 ttgaggtgcg gcgtttccat gcgcagccgc cgccagatct tgtccagccg ccaggccagt
      781 ggcggcagcc aggcgaagcc gctttcgatc agcaccagcc tgaagtcggg caggcgctcg
      841 aacacgccct cgatcaccag gctggtcagg aaggcctgcg ccgattgcgc gtgacccacc
      901 atgtcctcga tatagtagga cggccagccg gcgccgctca ccggatagcc gccgaagccg
      961 aaggcgtgca cgccgatcgg caggttcgct gcattggccg cctcgtacag cggccagtag
     1021 cgcttctggc ccggcggttc ggcggtgcgc gtcaccatca gcacctgcac gaagcgcgga
     1081 tcgcccgccc agcgctccac ttcgcgcgcc gcggcctccg gatcctcgaa cggtaccacg
     1141 atggagccct tcagccgggg ctccggtgcc agccaatgct ccaccagcca ttcgttcatc
     1201 gcgtgacaga cagcggcgcc gaattcgcgg tgctggaagg atgagccgtt gtcgccggtg
     1261 gcgttgagga tacccagggt gatgttgttg gcgtccagca actggcgctg catgaattcc
     1321 aggttgctgc ccggcttgcc gcccggcggc caggcatcgc gccgcgacgc attgggctgg
     1381 cctttcggat aggccgggcc ggcctggtac gcctggcgcg gccggttgcc gaaggtcagc
     1441 gcgtgctcct gccaacgctt ttccatccag gggaagatgt cgcggatgct gttgcgctgc
     1501 ggatggatgt cgccatcggc gatcgccagt ttcgacgtgt tgccggcggc gggagtcgga
     1561 cgggaagtga cctgcacgtt cattggaccg tctccgtcag gcgggcatag gtggcccgcg
     1621 gattgtcgat catgatcttg cgcacgagat cgggggaaag cccgtccggc agtgcgtctt
     1681 ggtcatcaaa ctgccagtgc gggtaatcgg tggagaacag cagcaactcg tcggacccca
     1741 tatggtcgag cagcttgccg aaggattccg catcgggcgg cgcgtcgcag ggttgggtgg
     1801 tcagccgcac attgctgcgc acgatgtcga tcggcgcgcg gtccacccag gggatttcca
     1861 tgcgcaaccc gcgccagaac ttggtcagcc gccacagatg cgccggcagc caggtgaagc
     1921 cggactcgat cagcacgacc ttgaggtccg ggaacttggt gaacacgcct tcgcagatca
     1981 ggctggtcag acattgctga aagccggcgg cctgggcggc gtaatcctcg gtgtagtagg
     2041 tcggccagcc gagcggcgtg accggatggc gataggcgct gccggcgtgg atgccgatgg
     2101 gcagattgtg gcgctgcgcc gccgcgtaga tcggccagta gtgtcgcttg cccagcggca
     2161 tgtcgcccat caccagcaac agcacctgca cgaagcgcgg atcgggggcg aggcgttcga
     2221 tctcgtccac cgccagttcc gggttctgcg ccggcacgac gatggaagcg cgcaggcgcg
     2281 gttcgcggtc cagccattcc gccgccatcc agtcgttgat ggcgcgggcg aaaccggcgc
     2341 ccatgtcctc gctgaacagc agttgcgcgc cgtacaggca gttgcagatg ccgatcgcgg
     2401 tgccgaacgg atcgagtgcg tgccggcgca accggtcgag atcggccccc ggctttctgc
     2461 cgtccacgcg ccagtccgga cgggcggtca gcggcgaatt ggcgggatag gcaatcgagt
     2521 ccagctcgtg cacgccgcgc tggatgacga tgtcgcgcca gtggtcggac aggtaggggt
     2581 gcagcgcctt caggttgggc accgcggggt gcagatcgca gtcgatagcg cctgtggcgc
     2641 ggacgctcat gcccgtcccc tctccttggc tccaaatgga acgctatcat gcgtcccggg
     2701 gaacggccag cagccgtgcc gagatgcaga agacgcgcgg tctcgacaat agccatcata
     2761 tagctatatt cctgccatga gcacgcatag cgtggccgag gccaagaacc acctgccaaa
     2821 gttgatcgac cgcgccctgg cgggggaggc ggtgctgatc acgcgtcatg gccagccggt
     2881 tgtggaactg aaagcaattc cagtccagcc gcgccgggtc tccccggaag acctggaatg
     2941 gctggctcgc cgtcgcaagg atttgcgcgt gccagtggag gatctcggcg cgttggtcag
     3001 tcgcatgcgc gatgaggact gggggtgagc gtctatctcg acgccagctt catcgtccca
     3061 ctgttcgcca accaggagcc gctgagcagg cgcgctgccg tctttcttgg tgattgcttg
     3121 ccggaacttg tcatcagcga cttcagcggt gcggaattcg catcggcgat tgcgcgcctg
     3181 acgcggatga atgcgctcac cacggatcag gcggaggcaa tcttcgcgga tttcgatatc
     3241 tggacgcgaa gggcgaccac aaacatcgaa acgattggcg cggacatcag agcggcggct
     3301 gcctatctcc gacaattgcg gttgaacctg cgcgccccgg atgcgctcaa catcgcggtt
     3361 gcccagcggg tgggcgccac gcttgcgacg ttcgatgatc gcatggcgga aaatgccgtc
     3421 cggctcggag tcgaggtggc cgcagtctga ccttggcccc gaaaccggcg ctgccaccga
     3481 cgggcgaaac ctgggtcaat ccaggtcccg cgccacctcc ggccggtgca tcagcgcgtc
     3541 gatcttcatc gcgtaatcca gcgcgtgatc cggctggaac gacagttgcg gcgcgaattt
     3601 cagccgcagc gcgtgcgcca cctgaccgcg cagatagacg ctcgcccgct tcagcgccgg
     3661 cagcagcgcg tccacatcgg tgcgcccgag ccgcgagacg aatacaatcg cgtgtttcag
     3721 gtcgggcgag gtgcgcactt ccgtcaccgt gatcacggca tcggccagtt ccggatcgcg
     3781 gaagtcgcgc cgctcgaaca gcccggcgag cacgtggcgg atttcctccg ctacgcgcaa
     3841 ttgccgctgg ctgggtcccg catcgcgctt cttcaagccg gcaccatctc cgtctcgaag
     3901 cactcgacca tgtcgccttc ctgcagatcg tggaagttcg caaaggacag gccgcactcg
     3961 tagccgcgcg ccacttcgcg cacgtcgtcc ttgaagcgct tcaactgcga caattcgccg
     4021 gtgtggatca ccacgttgtc gcgcagcagc cgcacgccgg cgccgcgctt caccaggccc
     4081 tcggtgacca tgcagccggc caccttgccc gtcttggtga tggtgaacac cttgcggatc
     4141 tcggcgtagc ccaggaactt ctcgcgcgcc ttgggtgcga ccttgccccg caccagcttc
     4201 tcgatgtcgt ccgccacctc gtagatgatc gcgtagtagc ggatatcgac accctcgcgc
     4261 tgcgccagct cgcgtgcctg cgcggtggcg cggacgttga acgcgacgat gaccgcgtcg
     4321 gaggccttgg cgagctggat atcgctttcg gtgatctggc ccacaccggc gatcagcacg
     4381 cggactttca cctcctcgtg cgccagcttc agtacggtgg cctggatcgc ctcggcgctg
     4441 ccctgcacgt cggccttgat caggacggcg acttccttct gctcgccggc ctggatgcgg
     4501 gccagcatct ggtccagcgt gccgcgtgcg ccggcgcttg ccgtcgccgc cttctcgcgc
     4561 agcttgcggg cgcggaattc ggtgatctcc cgcgcgcggc tttcactgtc caccaccacg
     4621 aagggctcgc cggcggtcgg cacgccggac aggcccagca cttccaccgg agtggacggc
     4681 tcggcgtcct tcagggcgcg cgccttgtcg tccagcatgg cgcgcacgcg gccccattcg
     4741 gcgccggcca ccaggatgtc gccggtatgc agcgtgcctt tctgcaccag gatggtcgcc
     4801 accggtccgc ggccgcgatc cagccgtgat tcgatgaccg agccttccgc cacgcggtgg
     4861 ggattggctt tcaggtcgag gatttccgcc tgcagcagaa tggcttcctg cagcttatcc
     4921 aggccggtct tcttcagggc ggagacttcc acgtcctgcg tctcgccgcc catgtcctcg
     4981 accaccacct catactgcag cagctcctgc cgcacgcggg tcgggttggc atccggccgg
     5041 tccatcttgt tgatggcgac gatgatcggg gcgttggccg ccttggcgtg gcggatcgcc
     5101 tcgatcgtct gcggcatcac gccgtcatcc gctgccacca ccagcacgac gatatcggtc
     5161 accgacgcgc cgcgggcgcg catggcggtg aacgcctcgt ggccaggggt gtcgatgaag
     5221 gtgatcttct cgccgctttc cagggtgacc tggtaggcgc cgatatgctg ggtgatgccg
     5281 ccggcttcgc cggccgccac gtcggtggag cgcagcgcgt ccagcagcga cgtcttgccg
     5341 tgatcgacgt gacccatgat ggtcaccacc ggcggacgcg cgaccagctc ggactcggtg
     5401 tccaggccgc cttccagtcc cagttcgaca tcggattccg acacccggcg cgcgcggtgg
     5461 ccgaattcct gcaccaccag ctcggcggta tcggcgtcca gtgtctgggt gatcgtcgcc
     5521 atgacgccga gcttcatcag cgccttgatg acgtcgccgc cgcgtgcggc catgcggttg
     5581 gcaagttcct gaacggtgat gttgtcgggc agaatgacgt cacgcacgac tttcacctga
     5641 tcggaacgca gccgctccag ctccgcctgc cggcgctcgc gttcgcgctg gcggcgcacc
     5701 gaggccagcg agcgcacccg ctcgtcttcg ccttcaatgg cagcctgaac gtcgatgcgg
     5761 cccgggcgcc ggcggtcttc gccggccttc ttcggagcca ccaccggcgg cttgcgcggc
     5821 ggggcgccgg cgccgggacg gcgggccgga cgtgcttcgt cctcatcttc cccggttcgc
     5881 gtacccggcc gcagccgcag cgtttcgctg ggcgtcgcac cagggcgctg cggcgcctgg
     5941 gtgggcgcac ggcgcggctc ctcgggtgcg cgctgggcgg gggcgcgggc ggccggggcg
     6001 gtggatttgg cggccgcggc ctgcgcggag gcggtagcct cggcgacctt gcgcgcctcc
     6061 tcctcggcct tttgctgcgc ctcctcctcc tccttgcggc gggcctcctc ttcggcggcc
     6121 ttgcgcgctt cctcctcgcg gcggcgggct tcctcggccg cggacaggat ggagatcttc
     6181 tcctgctcgc gccgctccgc ctcacgccgc gcggcttcgc gctgctgctc gaccagcgcg
     6241 cgctggcggg ccgcctgttc ggtctgggtc agcgcgcggg tgggcgcctg ctggccacgg
     6301 ccgccctggg acgggccgcg tgccggcgct gggccgccgc cactttggac gggggcagcg
     6361 ggcgcggggg cacccgcacg cttcttgcgc acttccacct ggacgacctt cgatcgccca
     6421 tgactgaaac tttgccgtac cgagccggcg tcaacagtgc gccccagctc caaacgtcct
     6481 gccggacgca gcgaaagacg gccgcccttg ccggtatcct gatcgttggt tccgctcatg
     6541 ctctatccgt tggcacccgt cgcttcgttc cgctgggcag cgttctgccc tgttcttctc
     6601 aatccggcca gccgctctgc ttcaacgcac agcgactcgg ccagctttcc gggtgcgatt
     6661 gccacatgca ccacatagtc acgtccgaac accctgccta gcgccgcccc aggcagcggc
     6721 gcgaaaaccg gcagcgtggc gccggacaac aatcgggcac gctccggttc gctaccatcc
     6781 gaggcctgca ccaccaggcg ggcacgcccg ttccgcaccc attcccgcgc cttgtcgaac
     6841 ccggcgacga cctggccggc ccggcgagag aggccgagaa gctcgccgat ccgccggatc
     6901 agcgccgctt gcagcacagc gaggagatcg gggggcacca ccaccgggcc gcgcgcggcc
     6961 cgcgcaaacg cacgcgtcag atttcccttc gcgctggcag tttctatcac atccccggct
     7021 gcgctcaacc agagtccccg gccgggcagc cttgcggtca ggtcggggac gacctgacgg
     7081 tccggcccga cgacgaagcg gatcatccgt tccttcggca gccgctcgcg cgtgagga
//
LOCUS       DASSZH010000429         3807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1407561, whole genome shotgun sequence.
ACCESSION   DASSZH010000429 DASSZH010000000
VERSION     DASSZH010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407561"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VFN42_09265"
     CDS             complement(<1..274)
                     /locus_tag="VFN42_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996844.1"
                     /translation="MPILATLPLLLAGTHLPASQNAPPANDQPLLTLPKVEVDQRIEP
                     FWKPKLQYLMPEVDGALITATTKATVTHMDQIPTVAQDNLTQLFVRT"
     gene            389..1318
                     /locus_tag="VFN42_09270"
     CDS             389..1318
                     /locus_tag="VFN42_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996845.1"
                     /translation="MSHKSTGALALNLALILALPQAAHACACGCGIFSVGAGNMLPST
                     LGNGTELFASVDYLDQTRDWSGSASAPASANADKNIRTLFQTVGVQTFFNRDWGLRVE
                     LPYWQRHFTTLDDAGQPVSFGHGALGDLRVLGLYTGFSPDRSSGLMFGAKLATGDWTY
                     ANFDRDTEIGTGTTDLLLGGYHQWKFGADGVWGAFVQTMADLPAGSRAGYRPGKELDT
                     AFGVYPQGWDIGGTHLTPVLQALLSLRGKDSGVQADPGNSGYQRLVAAPGMELAWQRL
                     RVDLSVGVPMYQHVNGNQLVAPWQGSLNLSYAL"
     gene            complement(1464..3716)
                     /gene="bglX"
                     /locus_tag="VFN42_09275"
     CDS             complement(1464..3716)
                     /gene="bglX"
                     /locus_tag="VFN42_09275"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF011678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HET8996846.1"
                     /translation="MPLRPVLLVLATALASLVSLPTLAATAAPASSTDPAAMRATVDG
                     LLARMTLMEKVGQLTILGADHKDLAALIRKGEVGGTNGVLPGVDVAAYTRRMQHLALQ
                     SRLHIPLLFMGDVVHGFRTIFPMPIGLAATWDPALVQQAMHAAAQEATRAGVDWTFAP
                     MVDIGRDPRWGRVSEGAGEDPYLDAVMTGAAIRGFQGKKLAAPDAMLATAKHFAGYGA
                     VQAGRDYNSVWLPRYLLDSVYLPPFKAAVDQGVGAVMAAFIALNAVPTTADGALIHGV
                     LRRQWGFDGLVVSDYDAVPQLQQHGIAATPAQAAQEAIDAGIDIDLHSGTYLKELPML
                     VREGKVPMATVDAAVRRVLEAKYRLGLFADPYRHGGRSGDSPMAPPSAAHRALAREVA
                     ARSMVLLCNQNHTLPLRSDLQRIAVIGPLADDRLDLLGPVHAEGQADEAVSVLAGIRQ
                     AVSPHTQVLYAPGTRVRGHSEAGFAQAVALARSADAAVLVVGEDVAIVGEGNSRSHLG
                     LPGNQLALVKAVVATGIPVVVVLINGRALALPWLASHVPAILDAWMPGTEGGPAVADV
                     LFGRVDPSGKLPVTFPRDLGQVPLFYSQLRTGRPYNPDDPYTTRYVDVANTPLFPFGY
                     GLSYTTFAIGDVHADRVRMPWNGTLTVTASIRNTGTRRGTDVVQLYVHDEVASVSPPL
                     RLLRGFRRVTLAPGEQHTLHFRLTRRDLAIVQSDLRWQANPGNYRIYIGDSSTATDHV
                     DVTLEAPATP"
BASE COUNT          566 a         1242 c         1355 g          644 t
ORIGIN      
        1 gcgtgcgcac gaacagttgc gtgaggttgt cctgcgccac ggtcgggatc tgatccatgt
       61 gcgtgaccgt ggccttggtg gtcgcggtga tcagcgcgcc gtcgacctcg ggcatcaggt
      121 actgcagctt gggtttccag aatggctcga tccgttgatc gacctcgacc ttgggcaagg
      181 tcagcagcgg ttggtcgttg gcgggcggtg cgttttgact cgcaggcaag tgtgtgccgg
      241 cgagcagcag tggcaaggtg gcgaggatgg gcatggtggt ctcggcaaac gcattggtgg
      301 gtgatgcggc aagatggcgc gggtctggag tctggatttc atccgaagtt tttatctaat
      361 cttcgacgcc aatgatggaa attcccagat gtcgcacaag tctaccggcg cgctcgcgct
      421 gaacctggct ttgatcctcg ctctgccgca ggcagcacat gcttgtgcct gcggctgcgg
      481 catcttcagc gtgggcgccg gcaacatgct gccgtccact ctcggcaacg gcaccgagct
      541 gttcgcgagc gtggactatc tcgaccagac cagggactgg tccggcagcg ccagcgcgcc
      601 ggcctcggcc aacgccgaca agaacatccg caccctgttc cagaccgtgg gtgtgcagac
      661 tttcttcaac cgcgactggg gcctgcgcgt cgaactgccg tactggcagc gccatttcac
      721 gacgctggac gatgctggac agccggtgag cttcggccac ggcgcactgg gcgacctgcg
      781 cgtgctgggc ctgtacaccg gcttctcgcc cgaccgttcc agcggcctga tgttcggcgc
      841 caagctggcc accggcgact ggacctatgc caacttcgac cgcgataccg aaatcggcac
      901 cggcaccacc gacctgctgc tgggcggcta ccaccagtgg aagttcggcg cggatggcgt
      961 gtggggcgcg ttcgtgcaga ccatggccga cctgccggcc ggcagccgcg ccggttaccg
     1021 gcccggcaag gagctggaca ccgccttcgg cgtctatccg cagggttggg acattggcgg
     1081 cacgcacctg acgccggtgc tgcaggcgct gctgtcgttg cgcggcaagg acagcggcgt
     1141 gcaggccgat ccaggcaatt ccggctacca gcgcctggtc gctgcgcccg ggatggaact
     1201 ggcctggcag cgcctgcgtg tcgacctcag cgtcggcgtg ccgatgtacc agcacgtcaa
     1261 cggcaatcag ctggtcgcgc cctggcaggg gtcgctcaat ctgagctacg ccttgtagtt
     1321 gcagttgcgt ggttgcagtt gccgtcggca tggcgacgtt cggcgttggt tgtcgcgggg
     1381 gagggcctgc tggtggggag tcgccgcagc cgcaggttgc cgggaccgca cgcaggtgtg
     1441 ctcaggcagt gcggtcgcat tcgctacggc gtggcgggtg cctccagagt gacgtccacg
     1501 tggtccgtcg cggttgagct gtcgccgatg tagatgcggt agttccccgg gttggcctgc
     1561 catcgcaggt cgctctgcac gatggccagg tcgcggcggg tcaggcggaa gtgcagcgta
     1621 tgctgctcgc ccggcgccag cgtcacgcgg cggaagccgc gcagcagccg cagcggcggg
     1681 ctgacgctgg ccacctcgtc gtgcacgtac agctgcacca catcggtccc cctgcgcgtg
     1741 ccggtgttgc ggatgctcgc ggtgaccgtc agcgtgccgt tccagggcat gcggacccgg
     1801 tcggcgtgca cgtcgccgat cgcgaacgtg gtgtagctga ggccgtagcc gaacggaaac
     1861 agcggcgtat tggccacgtc cacgtagcgc gtggtgtagg gatcgtcggg gttgtacggc
     1921 cgcccggtgc gcagctggct gtagaacagc ggcacctggc cgaggtcgcg cgggaaggtc
     1981 accggtaact tgcccgaggg atcgacgcgg ccgaacagca cgtcggcaac cgccggtccg
     2041 ccctcggtgc ccggcatcca ggcatccaga atcgccggta catggctggc cagccagggc
     2101 aacgccagtg cgcggccgtt gatcagcacc accaccacgg ggatgccggt ggcgaccacc
     2161 gccttgacca gggccagctg attgcccggc aggcccaggt gcgagcggct gttgccttcg
     2221 ccgacgattg cgacatcttc gcccaccacc agcactgctg cgtccgcgct gcgcgccagc
     2281 gccaccgcct gcgcgaagcc cgcctcgctg tggccgcgta cgcgggtgcc cggcgcgtac
     2341 agcacctgcg tgtgcggcga gacggcctgg cggatgccgg cgagcacgct cacggcttca
     2401 tcggcctgac cttcggcatg caccggaccg agcaggtcca ggcggtcgtc ggccagcgga
     2461 ccgatcacgg cgatgcgctg taggtcgctg cgcagcggca gggtgtggtt ctggttgcac
     2521 agcagcacca tcgatcgtgc cgccacttca cgtgccagtg cgcggtgtgc cgcggagggc
     2581 ggtgccatcg gcgagtcgcc gctgcgcccg ccgtggcggt agggatcggc gaacaggccc
     2641 agcctgtact tagcctccag cacgcggcgc acggcggcat ccacggtcgc catcggcact
     2701 ttgccttcgc gcaccagcat gggcagttcc ttcaggtagg tgccgctgtg caggtcgatg
     2761 tcgatgccgg cgtcgatggc ctcctgcgcc gcttgggccg gcgtggcggc gatgccatgc
     2821 tgctgcagtt gcggcacggc gtcgtagtcg gacaccacca acccgtcgaa gccccactgc
     2881 cggcgcaata ctccgtggat caacgcgccg tcggccgtgg tcggtacggc attcaatgcg
     2941 ataaaggcgg ccatcaccgc gcccacgccc tggtccaccg cggccttgaa cggtggcaag
     3001 tagaccgagt ccaacaggta gcgcggcagc cacaccgagt tgtagtcgcg gcccgcctgc
     3061 acggcgccgt agccggcgaa gtgcttggcc gtggccagca tggcgtcggg cgcggccagt
     3121 tttttgccct ggaagccgcg gatggcggcg ccggtcatca ctgcgtcgag gtaggggtct
     3181 tcgccggcgc cctcggatac ccggccccag cgcggatcgc gcccgatatc caccatcggc
     3241 gcaaaggtcc agtccacacc tgcgcgtgtg gcttcctgtg cggcggcatg catggcctgc
     3301 tgcaccagcg ccggatccca ggtggcggcc aggccgatgg gcatggggaa gatcgtgcgg
     3361 aagccgtgca ccacatcgcc catgaacagc agcgggatat gcagtcgcga ctgcagcgcc
     3421 agatgctgca tgcgccgtgt gtaggcagcc acgtccacgc ctggcagtac gccgttggtg
     3481 ccgccgacct cgcctttgcg gatcagcgcc gcaagatcct tgtggtccgc gccgaggatg
     3541 gtgagctggc cgaccttctc catcagcgtc atgcgcgcca gcaggccatc gacggtggcg
     3601 cgcatggctg ccggatcggt gctgctggcg ggggcggctg ttgccgcgag ggtgggtagc
     3661 gacacgaggc tcgccagagc ggtggccagc acgaggagga caggacgcag cggcatggac
     3721 ggattcccgg caaggcgatc ggcagccgtc gagcatagcg gcgggctggt gcgccggtgc
     3781 cgtacacgtg cttgtgctgc atggcat
//
LOCUS       DASSZH010000430         5534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_487381, whole genome shotgun sequence.
ACCESSION   DASSZH010000430 DASSZH010000000
VERSION     DASSZH010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487381"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VFN42_09280"
     CDS             complement(<1..827)
                     /locus_tag="VFN42_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HET8996847.1"
                     /translation="MSEAPTAPQDRDADLTGLTKRYAEHIAALRYADLPQDVVHKAKT
                     IIRDGVGNQIAASAISEPAARMIEIARGWGGTPEVTVTGYGLKLPAPMAVLCNAMLGH
                     GVELDDAHGTGLIKGGSVLVSLALASAELNGCSGEDVIAAVVGGYEIAIRIAKAINPG
                     HRQRGYHTTGTVSLIGGAALATKLLGGDAEQIACAIGLATMQSAGIQAFLDDSCMAKP
                     FSPGKGAMNGMMAAVMARSGFTGPRKALESREGFLNAFTSGVRASDLVEGLGSHYAI"
     gene            1281..2117
                     /locus_tag="VFN42_09285"
     CDS             1281..2117
                     /locus_tag="VFN42_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015073571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HET8996848.1"
                     /translation="MRISVVQMNPGADKAENIAQARRLIGQAVEADRPDIVSLPEVWD
                     SLGGDRAVRQANAEVLPAKGSNQPGGEAYEFLRETARATKTHVHGGSIIEQGPEKLFN
                     TTVVFNPDGTEIARYRKMHLFDIVAPDGTGYRESNTYGAGDEVVTYQADGTTVGCAIC
                     YDIRFPDLFWKLREQGAEVIFLPSSFTLATGKDHWETLIRARAIETQCWFAAPATWGK
                     HLEGKGEARFTYGHSMLVDPWGHTVAKVSDGIGWATARIEPSVTARVRRDMPVLEHRK
                     RL"
     gene            2171..2923
                     /locus_tag="VFN42_09290"
     CDS             2171..2923
                     /locus_tag="VFN42_09290"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013641183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HET8996849.1"
                     /translation="MHFAAASTPMATSARERLVARYGDCSRQRASVVVALGGDGSMLE
                     SLHRVLPLGIPVYGMNCGSVGFLMNTFSEDDLPGRLARAQEAELHPLHMRAVTKYRTV
                     VEGLAFNEVSLLRQLRQSAKIRITVDGRVRLEELTCDGVLISTPAGSTAYNLSAHGPI
                     VPLSANLLPLTPISAFRPRRWRGALLPSSAEVLFEVLEGDKRPTAAVADFTEVRHVVS
                     VSVQEDRSRHVTVLFDPDRGLSERIIAEQFTV"
     gene            complement(3045..3836)
                     /locus_tag="VFN42_09295"
     CDS             complement(3045..3836)
                     /locus_tag="VFN42_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996850.1"
                     /translation="MPERIADLLVFLRVLLAYGQHLTDSIEQRAASRGFGIIAQCFGT
                     TRVAAILARVARGMLRAIALERVLLARAARGRDLAPYKRRRPQEPDTQPAGQPQEEGA
                     AQGEAQGDEQAQGDAQAQEPPRPGRRPPPEPLPEFPTLEQLEAYTRRHPIGRAVAAIC
                     CDLGIASGLCEGWFTTVLYQAISWYRGNFPKYYKQIQARQAEFSEEWDRDTSARYDWP
                     SQSGEVVHRFLGFLIGENVLKPGPVPMLAGLAAAAVTTIAATGPP"
     gene            4173..>5534
                     /locus_tag="VFN42_09300"
     CDS             4173..>5534
                     /locus_tag="VFN42_09300"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET8996851.1"
                     /translation="MATDNNSRNQELLRQQAALAHFGQRALTSDDLDQILTEACRRIS
                     EALGTDLAKVMELQDDGETLLVRAGIGWKPGVVGVARTSASDNTSEGHALKTGGPMIS
                     PDIEQERRFIYPAFLVDNGVKAVANVPIIGCKGERPYGVLQVDSREPRQFTDSDTTFL
                     TTYANLLAAAVTRLRVTRNLQEGERRLRRSEERLRRVTEIETVGVLFFDAAGRITDAN
                     AAFLRMSGLSREDIAAGRVHWEELTSPEWMPATLRALEEMKATGHGKPFEKQYVRKDG
                     SRWWGLFAGRMLDDGGAVEFVLDVTDRKAADQALRASEARLRALVEGIPQLVFRSRSS
                     GARIWGSSQWVNYTGLGEAQSLGLGWLDAVHPEDRPATLDAWQDAEAIGRVSIEHRTR
                     RASDGAWRWFQTRAMPVRNEAGHIIEWFGTSTDIDDQVRARDVLARNRDELEVLVAER
                     TEAL"
BASE COUNT          877 a         1817 c         1957 g          883 t
ORIGIN      
        1 atgatcgcgt agtggctgcc cagtccttcc accagatcgc tcgcgcgcac accgctggtg
       61 aaggcgttca ggaagccttc gcgcgactcc agcgccttgc gcggtccggt aaagccgctg
      121 cgcgccatca ccgccgccat catgccgttc atcgcaccct tgccggggct gaacggcttg
      181 gccatgcagg aatcgtccag gaacgcctgg atgccggcgg actgcatcgt cgccaggccg
      241 atcgcgcagg cgatctgctc cgcatcgccg ccgagcagct tcgtcgccag cgcggcgccg
      301 ccgatcaagg agaccgtgcc ggtggtgtgg tagccgcgct gccgatggcc agggttgatc
      361 gccttggcga tccggatggc aatctcatag ccgccgacga cggcagcgat gacgtcctcg
      421 cccgagcagc cattcagttc ggcgctggcc agcgccagcg acaccagcac cgagccgcct
      481 ttgatcagcc cggtgccatg cgcgtcatcc agttccaccc cgtgcccgag catcgcgttg
      541 cacagcaccg ccatcggcgc cggcagcttc aggccatagc cggtgacggt cacttccggc
      601 gtgccacccc agccgcgggc aatctcgatc atgcgggctg ccggctcgct gatcgccgag
      661 gcggcgatct gattgccgac gccgtcgcga atgatggtct tggccttgtg aaccacatcc
      721 tgcggcagat cggcgtaacg cagggccgca atgtgttccg catagcgctt ggtcagaccc
      781 gtcaggtccg cgtcgcggtc ctgcggagcg gtgggggctt cgctcatcat cgcttcctct
      841 cttcttaggc ggcgtagctg cttttactgc taatatccct cggcgccacg cctgacaacc
      901 ccggggcaga ggatccgcat ctggagggac agcctcgtaa ggggtccggc gtgacctcca
      961 atgcctcaga aggcaccctc cagaagctgg aggcctgatt ggtccgccag ggtcgccgcg
     1021 tgacagcgca aggctgagcc ggatgctttc gctggccgca gcgtggctgt atcaggtccg
     1081 gatcgacctg cgggcgcgtg taccacgcgc caaaaaccag gctatacccg gcatgcgacc
     1141 gcccccgagc gtcgcgaagc gcggcgatgc ggtggccgta tcggttgccc cacaggctca
     1201 tcgcggcgac ccgggtgttg tcggattgcc atcccccctg cgatgggctt ccctgcggcc
     1261 gatcatcctg gagcggaccg atgcggatct ccgtcgtgca gatgaacccg ggcgccgaca
     1321 aggcggagaa tatcgcccag gcccgccgcc tgatcggcca agcggtggag gccgaccgtc
     1381 ccgatatcgt cagcctgccc gaggtctggg acagtttggg cggcgatcgt gccgtccgcc
     1441 aggccaatgc cgaggtgctg ccggccaagg gttccaacca gcccggtggc gaggcctatg
     1501 aattcctgcg ggagaccgcc cgcgccacga agacccacgt gcatggcggc tcgatcatcg
     1561 agcaggggcc ggagaagctg ttcaacacta ccgtggtgtt caacccggat ggcaccgaga
     1621 tcgcccgcta ccgcaagatg cacctgttcg acatcgtcgc cccggatggc accggctacc
     1681 gcgaaagcaa cacctacggc gcgggggacg aggtggtgac ctatcaggcc gacggcacca
     1741 cggtcggctg cgcgatctgc tatgacatcc gctttccgga cctgttctgg aagttgcgcg
     1801 aacagggcgc cgaggtgatc ttcctgccct cgtccttcac cctggctacc ggcaaggacc
     1861 actgggagac gctgatccgc gcccgggcga tcgagacgca atgctggttc gccgcgcccg
     1921 ccacctgggg caagcatttg gaaggcaagg gcgaggcgcg cttcacctac ggccactcca
     1981 tgctggtcga tccctggggc cacacggtgg cgaaggtgtc ggacggcatc ggctgggcca
     2041 ccgcgcgcat cgagccgagc gtgaccgcgc gggtgcggcg cgacatgccg gtgctggaac
     2101 accggaagcg cctttgaacg agaccacgca ggcggtgacc ctgggtcagc ggcattacgt
     2161 gccggggcgc atccatttcg ccgcagcctc caccccgatg gccaccagcg cccgcgagcg
     2221 gctggtggcg cgctatggcg actgctcgcg gcagagggcg tcggtggtcg tggcgctggg
     2281 cggcgacggc tccatgctgg aatcgctgca ccgcgtgctg ccactgggca tcccagtgta
     2341 cggcatgaac tgcggctcgg tcggcttcct gatgaacacc ttcagcgagg acgatctgcc
     2401 cggtcggctg gcccgcgcgc aggaggcgga actgcatccg ctgcacatgc gcgcggtgac
     2461 aaaataccgc acggtggtgg aggggctggc gttcaacgag gtcagcctgc tgcgccagtt
     2521 gcgccagagc gcgaagatcc gcatcacggt ggacgggcgc gtgcggctgg aggaactgac
     2581 ctgcgacggc gtgctgattt ccacgccggc cggatcaacc gcctataacc tgtcggcgca
     2641 cggcccgatc gtcccgctct ccgccaacct gttgccgctg acgccgatct cggcgttccg
     2701 tccccggcgc tggcgcggtg ccttgctgcc cagctcggcg gaggtactgt tcgaagtgct
     2761 ggagggcgac aagcgcccca ccgccgcggt ggccgacttc accgaggtgc ggcatgtggt
     2821 ttcggtgtcg gtgcaggagg atcggtcacg ccacgtgacg gtgctgttcg atccggaccg
     2881 cggcctgtcg gagcgcatca tcgcggagca gttcacggtc tgacgtctgt tccggctcgg
     2941 ccggctgcgg ccgccgcgcg ccgcgccgca gtacacgcga atcggaacgg cggggccggc
     3001 cgaacccggc cccgagcgtt ccctgcgaca ggcgtggggc cgagtcaggg cgggccagtg
     3061 gcggcgatgg tggtgacggc ggcagcggcg aggccggcca gcatgggcac cgggcccggc
     3121 ttcagcacgt tctcgccaat caggaagccc aggaagcggt ggaccacctc gccgctctgc
     3181 gacggccagt cgtagcgggc actcgtgtcg cggtcccact cttccgagaa ctctgcctgc
     3241 cgcgcctgaa tctgcttgta gtatttgggg aaattgccgc gataccagct tatcgcctgg
     3301 tacagcaccg tggtgaacca gccctcgcac aggccggacg cgatgccgag gtcgcagcag
     3361 atcgcggcga ccgctcggcc gatcgggtgg cggcgggtgt atgcctccag ctgctccagc
     3421 gtggggaatt ccggcagcgg ctccggcggc ggacgccggc cgggacgcgg cggctcctgc
     3481 gcctgtgcgt ccccctgcgc ctgttcatcc ccctgcgcct cgccttgcgc cgctccttcc
     3541 tcctgcggct ggccggccgg ctgggtgtcg ggttcctgcg gccggcggcg cttgtagggg
     3601 gccaggtcgc ggccccgcgc ggcgcgggcg agcagcacgc gttccagtgc gatggcgcgc
     3661 agcatgccgc gggcgacgcg ggcgaggatg gcggcgacgc gggtggtgcc gaagcactgg
     3721 gcgatgatgc cgaagccgcg gctggcggcg cgctgttcga tgctgtcggt gaggtgttgt
     3781 ccgtaggcga gcaggacgcg gaggaagacg agaagatcgg cgatgcgttc gggcacgggc
     3841 ctgctggtgg ggccctggcg gggaccttgt cgggtagaaa gaccagcagt ggggttcgcg
     3901 tgcttcatgg gtagtgtatc taacacacaa atgtgaacct tgtcaaggga gatcttccgg
     3961 cggcagtgcg ccgggagcat ccgccaggca tcgcgtgccg cgagtgttga tccaatgtca
     4021 tgcccgcccc gcgtgcggcg tgcgtcggtc agccaaacgg accgcacccg ttcgcgtttc
     4081 gactcctccg gtccagccac ggatcggagg gcagagcggc ccggagcgga cgacgttccg
     4141 agtggcggat cagatgaagt gcgagggcgt cagtggcgac ggacaacaac agcagaaatc
     4201 aggaactgct gcggcagcag gctgcgctcg cacatttcgg ccagcgcgcg ctcacctccg
     4261 atgacctgga ccagatcctt accgaagcct gccgccgcat cagcgaggcc ctcggcaccg
     4321 atctcgccaa agtgatggaa ttgcaggatg acggagagac cctgctggtg cgtgccggca
     4381 tcggctggaa gcctggcgtg gtgggggtgg cgcgcaccag cgcatcggac aacacatcgg
     4441 aaggccatgc gctgaaaacc ggtgggccca tgatctcccc cgacatcgaa caggaacggc
     4501 ggttcatcta tccggcattc ctggtggaca acggcgtcaa ggcggtggcg aacgtgccga
     4561 tcatcgggtg caagggcgag cggccttacg gcgtgctgca ggttgacagt cgcgaaccgc
     4621 ggcagttcac cgacagcgac accacctttc tcaccaccta cgccaacctg ctggccgccg
     4681 cggtgacgcg gctgcgggtg accaggaacc tgcaggaggg tgagcggcgc ctgcgtcgca
     4741 gcgaggaacg gctgcgccgg gtgacggaaa tcgagacggt gggtgtgctg ttcttcgatg
     4801 cggcggggcg tatcaccgac gccaatgcgg ccttcctgcg catgtccggg ctttctcgcg
     4861 aggacatcgc ggcaggccgg gtgcactggg aagaactcac atcgccggaa tggatgccgg
     4921 ccaccttacg cgcgctggag gagatgaagg ccaccggcca cggcaagccg ttcgagaagc
     4981 agtatgtgcg caaggatggg tcgcgctggt gggggctgtt cgccggacgg atgctggacg
     5041 acgggggcgc tgtcgaattc gtgctggacg tcaccgaccg caaggccgcc gaccaggcgc
     5101 tgcgtgccag tgaggcgcgg ttgcgcgcgc tggtggaggg catcccgcag cttgtgttcc
     5161 gctcgcgcag ctccggcgcg cgcatctggg gcagttcaca gtgggtgaat tataccgggc
     5221 tgggggaggc acaaagcctg ggcctgggct ggctcgacgc ggttcacccg gaggaccggc
     5281 cggcgacctt ggacgcatgg caggatgccg aggcaatcgg ccgggtgtcc atcgagcacc
     5341 gcacgcgccg agcctcggat ggcgcgtggc gctggtttca gacgcgcgcg atgccggtgc
     5401 gcaacgaggc agggcacatc atcgaatggt tcggcacttc caccgatatc gacgaccagg
     5461 tgcgcgcccg ggatgtgctg gcccgcaatc gcgacgagct tgaggtcctg gtggcggagc
     5521 ggaccgaggc actg
//
LOCUS       DASSZH010000431         2924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1697987, whole genome shotgun sequence.
ACCESSION   DASSZH010000431 DASSZH010000000
VERSION     DASSZH010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1697987"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VFN42_09305"
     CDS             <1..331
                     /locus_tag="VFN42_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010514335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosomal replication initiator DnaA"
                     /protein_id="HET8996852.1"
                     /translation="VLLAAELPPARWPVRLPDLASRLRAIMAVEIGQPEDSLLDALLL
                     RLTAERQLDLPPALQTWLRRRLPRSPGALREAVDRLDRAGLAAGGKISRPMAAEALAD
                     LLSDTEN"
     gene            388..2634
                     /locus_tag="VFN42_09310"
     CDS             388..2634
                     /locus_tag="VFN42_09310"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HET8996853.1"
                     /translation="MPRSSAHAAASAETAERISPRAAPPHPALTGLPRITPDSPQRFV
                     NRELSWLDFNHRVVEEAENARHPLLERLRFVSISASNLDEFYSVRVAGLVGQAKAGLT
                     TISPDGRSPAQQLAEIRLRAESLLAGQQRVWRELRGLLRQAGIEVCDPAGLTEEDRRW
                     LDAWFMERVFPVLTPLAIDPAHPFPFIPNMGLVMALLLLREEDAQGMRGLIPLPSQVE
                     RFIRLPAAAPGQSIRFVMLEDLVGMFFDRLFPGFRVTGQGMFRLIRDTDVEFEEEAED
                     LVRSYESALKRRRRGVVIHLGMDARMPDELRALVADQLDATDEEIHVADGILGVADLR
                     QLIVDDRPDLLFPPYTPRFPERIRDFGGDCFAAIRAKDIIVHHPYESFDVVVQFLRQA
                     AQDPAVIAVKQTLYRTSRDSPIVKALIEAAEAGKFVTAVVELRARFDEEMNIRLARSL
                     EAAGVQVVFGFAELKTHAKLSLVVRREGSSVRSYAHFGTGNYHPITARIYQDLSFFTS
                     DPALTRDAARLFNYMTGYARPAQMEEVAFSPLTTRPVLLELIRKEIAFAHAGRPAGIW
                     IKLNSLVDKELIDALYEASAAGVKIMAVIRGICCMRPGVPGLSDNIRVKSIVGRFLEH
                     SRICVFGNGHALPSRHARVYISSADWMERNMDWRVESLVPIHNPTVHAQVLDQIMVVN
                     LKDTMQSWELKPDGAWRRVPPGKNPVSAHDYFMTNPSLSGRGSALHGPAVPSPLAMHR
                     RQDRVNQD"
     gene            2639..>2924
                     /locus_tag="VFN42_09315"
     CDS             2639..>2924
                     /locus_tag="VFN42_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HET8996854.1"
                     /translation="MPFAPPPGLPRCGVVDLGSNSVRLVVFEGECRNPVAIFNEKAVL
                     RLGRGLQTTGQLNPEAMEQAFTVMHRYHAVARAMGATPFEVLATAAVRDAS"
BASE COUNT          440 a         1022 c          997 g          465 t
ORIGIN      
        1 ggtgctgctc gccgccgagc tgccgccggc gcgctggccg gtgcgcctgc ccgacctggc
       61 cagccggctg cgcgccatca tggcggtgga gatcggccag ccggaagatt cgctgctgga
      121 cgccctgctg ttgcggctga cggcggagcg gcagctggac ctgccgccgg cgttgcagac
      181 ctggttgcgt cgccgcctgc cgcgttcccc cggcgccttg cgggaggcgg tcgatcggct
      241 ggaccgcgcg ggcctcgccg ccgggggaaa gatcagccgg ccgatggcgg ccgaagcgct
      301 ggccgatctg ctgtcagaca cagaaaatta gcccgtcgga ggtaaacgtc ctcgccgccc
      361 ttggggcgcc tcctatagag tcgggtcatg cccaggtcct ccgcccacgc cgccgcatcc
      421 gccgagacgg cagaacgcat ctcgccgcgt gcggctccac cgcatccggc gctcaccggt
      481 ctgccacgca tcacgccgga cagtccgcaa cgcttcgtca atcgcgaact gtcctggctg
      541 gacttcaacc accgcgtggt ggaggaggcg gagaacgccc gccatcccct gctggagcgg
      601 ctgcgcttcg tctcgatcag cgcctccaac ctggacgagt tctattcggt gcgcgtcgcc
      661 ggcctggtcg gccaggccaa ggcggggttg accacaatct ccccggacgg ccgcagcccg
      721 gcgcagcaac tggcggaaat ccgcctgcgc gccgaatcgc tgctggccgg acagcagcgc
      781 gtgtggcggg aattgcgcgg cctgctgcgc caggccggca tcgaggtgtg cgacccggcc
      841 gggctgaccg aggaagaccg ccggtggctc gacgcctggt tcatggagcg ggtgttcccg
      901 gtgctcaccc cgctggcgat cgatccggcg cacccgttcc cgttcattcc caacatgggc
      961 ctggtgatgg cgctgctgct gctgcgggag gaggacgcgc agggcatgcg cggcctgatc
     1021 ccgctgccca gccaggtgga gcgcttcatc cgactgccgg ccgcggcgcc cggccagtcg
     1081 atccgcttcg tgatgctgga agacctggtc gggatgttct tcgaccggct gtttcccggc
     1141 ttccgcgtca ccggccaggg gatgttccgg ctgatccgcg acaccgacgt ggagttcgag
     1201 gaagaggcgg aagacctggt ccgctcctat gaatccgcgt tgaagcggcg gcgccgcggc
     1261 gtggtgatcc atctgggcat ggacgcccgc atgccggacg aactgcgggc actggtcgcc
     1321 gaccagctcg atgcgacgga tgaggaaatc cacgtcgccg acggcatcct gggggtggcc
     1381 gatctgcgcc agctgatcgt ggacgaccgg cccgacctgc tgtttccgcc ctataccccg
     1441 cgcttcccgg agcgcatccg cgacttcggc ggcgactgct tcgccgccat ccgcgccaag
     1501 gacatcatcg tccaccaccc gtacgagagc ttcgacgtgg tggtgcagtt cctccgccag
     1561 gcggcacagg accccgcggt gatcgcggtg aagcagacgc tgtaccgcac cagccgcgac
     1621 agcccgatcg tcaaggcgct gatcgaggcg gcggaggcgg gcaagttcgt caccgccgtg
     1681 gtggagctgc gcgcccggtt cgacgaggag atgaacatcc gcctcgcccg cagcctggaa
     1741 gccgccggcg tgcaggtggt gttcggcttc gccgaactga agacgcacgc gaaactgtcg
     1801 ctggtggtgc gccgggaggg cagttccgta cgctcatacg cccatttcgg caccggcaat
     1861 taccacccga tcaccgcgcg catctaccag gacctgagct tcttcacctc cgaccccgcc
     1921 ctgacgcgcg acgccgcccg gctgttcaac tacatgaccg gctatgcgcg cccggcgcag
     1981 atggaggagg tggcgttcag cccgttgacc acgcggcccg tgctgctgga gctgatccgg
     2041 aaggagatcg ccttcgcgca tgccggccgc cccgccggca tctggatcaa gctgaactcc
     2101 ctggtggaca aggagctgat cgacgcgctg tacgaggcgt ccgccgccgg ggtgaagatc
     2161 atggcggtga tccgcggcat ctgctgcatg cggcctggcg tgcccggact gtcggacaac
     2221 atccgggtga agtcgattgt cggccgcttc ctggaacaca gccggatctg cgtgttcggc
     2281 aatggccacg ccctgcccag ccggcacgcg cgggtgtata tcagctccgc cgactggatg
     2341 gagcgcaaca tggactggcg cgttgagtcc ctggtgccga ttcacaatcc gaccgtgcac
     2401 gcgcaggtgc tcgatcagat catggtggtg aacctcaagg acaccatgca gtcctgggaa
     2461 ctgaagccgg acggtgcctg gcggcgcgtt ccgccaggga agaacccggt ttcggcgcat
     2521 gactacttca tgaccaaccc ctcgctgtcc ggccgcggat cggcgctgca cggcccggcg
     2581 gtgccctcgc cgctggccat gcaccggcgg caggaccggg tgaaccagga ctgaccgcat
     2641 gcccttcgca cccccccccg gattaccgcg ctgcggcgtg gtggacctcg gttccaactc
     2701 ggtgcgtctg gtcgtgttcg aaggcgagtg ccgcaatccg gtagcgatct tcaacgagaa
     2761 ggccgtgttg cgcctgggcc gcggcctgca gaccaccggc cagctgaacc cggaggcgat
     2821 ggagcaggcc ttcaccgtca tgcaccgcta ccacgcggtg gcacgggcga tgggcgccac
     2881 gcccttcgag gtgctggcca ccgccgcggt gcgcgacgcc tcca
//
LOCUS       DASSZH010000432         3100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1390945, whole genome shotgun sequence.
ACCESSION   DASSZH010000432 DASSZH010000000
VERSION     DASSZH010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390945"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..162
                     /locus_tag="VFN42_09320"
     CDS             <1..162
                     /locus_tag="VFN42_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HET8996855.1"
                     /translation="LAGGEWNGKAGGYAIQGHAAAFVRFLSGSHSNVVGLPLFETAQL
                     LRGFGLLAP"
     gene            159..1250
                     /locus_tag="VFN42_09325"
     CDS             159..1250
                     /locus_tag="VFN42_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007399872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HET8996856.1"
                     /translation="MSVRILASASPGEVRVAAARGDRLLDYALWRPGRPDGVGDLYRG
                     RVSARVPAMAGVFVALEGAEGFLPDSEGGKHLTEGTVLAVCVTRAAQGGKGPRLSAKL
                     ADADRRLGGSGSPALLRRGPGPVERFAAQYPEAPVLVDDAALLAELRPRLGERIRVAA
                     EPLDDALENAIAELAEPEVILPNGARLAIHPTPALVAIDVDAGAAIAEQRGKAERHFA
                     VNRAMLPDLAAQIRLRNLSGAILMDFAGLHVRQRGRLGPDLEAALAADPLRPRLLGFT
                     ALGLAEIVRPRVHPPLHELLVGPHATGLAALRAVARRAAAEPAWLPELRATPSVVRAL
                     QDDPVALEDLRRRTGRAVQLHADPGLTGR"
     gene            1462..1851
                     /locus_tag="VFN42_09330"
     CDS             1462..1851
                     /locus_tag="VFN42_09330"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET8996857.1"
                     /translation="MSIGRKRRSFTRDFKLSVLKRMAETDNIRGLAAELGIERRLLYC
                     WRDAFAVSGEAGLRRAGRPSAADRAEAALAVHQGGQVPDPAQRVAELERKVVEQQVAL
                     DFLRAALRHVRAQRRKTGVPRGKASTP"
     gene            2103..2285
                     /gene="yacG"
                     /locus_tag="VFN42_09335"
     CDS             2103..2285
                     /gene="yacG"
                     /locus_tag="VFN42_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019089265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HET8996858.1"
                     /translation="MSTRPTSLCPVCGRPATIDARPFCSPRCADIDLGRWVTGQYRIP
                     APADAYDDQASDPPEC"
     gene            complement(2295..>3100)
                     /locus_tag="VFN42_09340"
     CDS             complement(2295..>3100)
                     /locus_tag="VFN42_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET8996859.1"
                     /translation="RLRNTLRWVLGSLAGFTEDERVPVNAMPELERWVLHRLTELDAR
                     IRAATESYAWTGVYPELHNFCATDLSAFYFDIRKDAIYCDRPDSHRRRAARTVLDHLH
                     RCLTTWLAPVLCFTAEEAWGARFGADDSVHLQQFPELPAEWRDDRLAAKWDQIRGIRR
                     RITVPLDEARKTNVLGSSLQASASLPLAADEAELLNAAEWAEIAIVSAVHPVAGSADD
                     LATITPAPGEKCARCWRVLPEVGSDQKHPTLCLRCADAVESGLVCRPAA"
BASE COUNT          451 a         1056 c         1136 g          457 t
ORIGIN      
        1 ctggccggcg gcgagtggaa cggcaaggcg ggcggctatg ccatccaggg ccatgcggcc
       61 gccttcgtgc ggttcctgtc cggcagccat tccaacgtgg tcgggctgcc gctgttcgag
      121 accgcccagc tgttgcgcgg cttcggcctg ctggcgccgt gagcgtccgc atcctggcca
      181 gcgccagccc gggcgaagtg cgcgtcgccg cggcgcgcgg tgaccggctg ctggattacg
      241 cgctatggcg gccagggcgg ccggatggag tgggtgacct gtatcgcggc cgggtttccg
      301 cgcgggtgcc cgccatggcg ggggtattcg tcgcgctgga gggggccgag ggcttcctgc
      361 cggacagcga aggcggcaag cacctgaccg aaggcaccgt tctcgccgtc tgcgtgactc
      421 gcgcggcgca gggcggcaag ggcccgcggc tgagcgcgaa gctcgccgac gcggaccggc
      481 gcctgggcgg aagcggctcc cctgccctgc tccgccgcgg cccgggaccg gtggaacgat
      541 tcgccgcgca gtatcccgaa gcgcccgtgc tggtggacga tgccgccctg ctggccgaac
      601 tgcggccgcg cctgggtgaa cggatccggg ttgccgcgga gccgctggac gatgcgctgg
      661 agaacgcgat cgccgaactg gcagagcccg aggtgatcct gccgaacggc gccaggctgg
      721 caatccatcc caccccggcg ctggtggcga tcgacgtgga tgccggcgct gcgatagccg
      781 agcagcgcgg caaggcagag cggcatttcg cggtcaaccg cgcgatgctg ccggacctcg
      841 cggcgcagat ccggctacgc aacctatccg gcgccatcct gatggatttt gccggcctgc
      901 acgtgcggca gcgcggccgc ctcggccccg acctggaagc ggctctcgcc gccgatccgc
      961 tgcgtccgcg cctgttgggc ttcaccgcgc tcgggctcgc cgaaatcgtg cgcccgcgcg
     1021 tgcatccgcc gctgcacgag ctgctggttg ggccgcacgc cacggggctg gcggccttgc
     1081 gcgcggtggc acggcgagcc gcggcggaac cggcttggct gccggagctg cgcgccacgc
     1141 catccgtcgt gcgggccttg caggacgacc ccgtggcgct ggaggatttg cgccgccgca
     1201 ccggccgggc agtgcaattg cacgccgacc cgggattgac cgggagatga cggctcccct
     1261 ccaatgcgag ggagcgattg cggcagcagc aataaaatgg aaccgcagat gaacgcggat
     1321 gaacgcggat gatcctcggc gcccgatcgg ctgggtgtcg ggccttcgcc cgccacgacg
     1381 cggtactgct ggcggtgtgt catgagcaaa gcgggctggt atgagacacg agatttagac
     1441 acactgttcg ggggaacgga tgtgtcgata gggcgaaagc ggcgcagctt cacgcgcgac
     1501 ttcaagctgt ccgtgctgaa gcggatggcg gagacggaca acatcagagg gctggcggcc
     1561 gaactcggga ttgagcgcag actgctgtat tgctggcgcg acgccttcgc ggtgagcggc
     1621 gaggctggcc tgcgccgggc cggccggccg agcgcggcgg atcgggcgga agccgcattg
     1681 gcggttcacc agggcgggca agtgccggat ccggcccagc gcgtggcaga gctggagcgc
     1741 aaggtcgtcg aacagcaggt ggcgctggat tttcttcgcg ccgccttgcg gcatgtcagg
     1801 gcgcaacgcc ggaagaccgg cgtgcctcgc gggaaggcat ctacgccatg atccaggcgt
     1861 cgatgcagca gcaagcccgc cttgggatcg aagccccgtg tgcgatggct gtcgtcagcc
     1921 gggctggcca ttacgtcact gccgggcgag cgcgcggcgt gaggatgaga cggaggtgcc
     1981 gcgatgcgat ccagcggcat tccatcaggc ggcgggcatg ctactggcgg gacgggccaa
     2041 ccgcccccac ttcaccgtga ccacctccct tcccggggga aatgcaccgt gcaggaccgg
     2101 ccatgagtac acgccccact tcactttgcc ctgtctgcgg ccggccggcg acgatcgatg
     2161 ccaggccgtt ctgcagcccg cgctgcgccg atatcgatct gggccgctgg gtgaccgggc
     2221 agtaccgcat acccgcgccc gcggacgcgt atgatgacca ggcctccgac ccgccggagt
     2281 gctgagcgcg ccggtcaggc tgccgggcgg cagaccaggc cggattccac cgcatcggca
     2341 cagcgcaggc acagcgtcgg atgcttctgg tccgatccga cttccggcag cacccgccag
     2401 cagcgcgcgc atttctcgcc cggcgccggg gtgatcgtgg ccaggtcgtc ggcactgccg
     2461 gccaccgggt gcactgcgga gacgatggcg atctcggccc attccgccgc gttgagcagc
     2521 tccgcttcgt ccgccgccag cggcaagctc gccgacgcct gcagtgacga acccagcacg
     2581 ttggtcttgc gcgcctcgtc cagcggcacg gtgatgcggc ggcggatgcc gcggatctgg
     2641 tcccatttcg ccgccagccg gtcgtcgcgc cactccgccg gcagctccgg gaactgctgc
     2701 aggtggacgc tgtcatcggc gccgaaccgc gcgccccagg cttcctcggc ggtgaagcac
     2761 agcaccggcg ccagccaggt ggtgaggcag cggtgcagat ggtccaacac cgtgcgcgct
     2821 gcgcgccggc ggtggctgtc cggacggtcg cagtagatcg catccttgcg gatgtcgaag
     2881 tagaacgcgg acaggtcggt ggcgcagaaa ttgtgcaact ccggatatac cccggtccag
     2941 gcgtagcttt ccgtggcggc gcggatgcgc gcatccagtt cggtcagccg gtgcagcacc
     3001 cagcgctcca gctccggcat cgcgttcacc ggcacgcgtt catcctcggt gaagccggcc
     3061 aggctgccca gcacccagcg cagcgtgttg cgcaggcggc
//
LOCUS       DASSZH010000433         2558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_947845, whole genome shotgun sequence.
ACCESSION   DASSZH010000433 DASSZH010000000
VERSION     DASSZH010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947845"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VFN42_09345"
     CDS             complement(<1..707)
                     /locus_tag="VFN42_09345"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET8996860.1"
                     /translation="MARAAIMAAGLAASLCGCAHDKPAPLSAAANAQALESRSLRDPR
                     LLTFVQAALARHDSASSRHAWDLARLTLAALYFHPDIPIAQARLAAAKAAELTARERP
                     NPVLNLTNIIDQAAVPGALPAAAAAITIGPVIDFVVETAGKRAARTARARHLAEAAGW
                     DLTTAEWQVRSRVRNALLDLWAARQRLARSRQQQALQQALVQLLQIRLSAGEASAPEL
                     DRERIRYARIIVKRSDLR"
     gene            943..1557
                     /locus_tag="VFN42_09350"
     CDS             943..1557
                     /locus_tag="VFN42_09350"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET8996861.1"
                     /translation="MDRLIAGYHRFRQSRWPERKATYESLAEHGQAPRALVLACSDSR
                     VDPTMIFDAAPGELFIVRNIANLVPPYQPDARWHGTSAALEFGVRVLQVPDLIVMGHA
                     MCGGINALLNGVPPECGDFLASWIKIAEPAKRRVLQCAPADPLTAAEHEAVKLSLENL
                     LSFPWIAERVQSGKLTLHGAMYGIRSGELSLLGDDGAFHAVPTA"
     gene            complement(1554..1970)
                     /locus_tag="VFN42_09355"
     CDS             complement(1554..1970)
                     /locus_tag="VFN42_09355"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8996862.1"
                     /translation="MLVFDLSAEQVFDPRKHVEKVLGRVGEGDVTIACWEPGQTSPNH
                     LHPNATEIYFCHEGGGVMWTPTQTVDVKPGCFVVHPRGELHEYRNGPQRTVLFRVRYG
                     DDMSTRTRDWPGNPGYVAKPEDAAWFAAHPEPAQPG"
     gene            complement(1985..2386)
                     /locus_tag="VFN42_09360"
     CDS             complement(1985..2386)
                     /locus_tag="VFN42_09360"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HET8996863.1"
                     /translation="MSYAKPLPRIDTLNQPFWEAARQNRLVVQYCTACGDTRFPPGPV
                     CPRCLSPEQSWKDVSGRGTLQSWVEFHRAYWDGFKPGLPYKVCLVQLEEGPLLVSNLV
                     GSDANAKLGAPVRVVFEPATDEITLPKFTLA"
BASE COUNT          418 a          845 c          857 g          438 t
ORIGIN      
        1 cgcagatcgc tgcgcttgac gatgattcgc gcatagcgga tgcgttcgcg atccagctcc
       61 ggcgccgagg cttcgccggc cgaaaggcga atctgcaaga gctgaaccag cgcctgctgc
      121 agcgcctgct gctgccggct gcgtgcaaga cgctgccgtg cggcccacag atccagcagc
      181 gcattgcgca cccggctgcg cacctgccat tccgcggttg tcagatccca gcctgcggct
      241 tccgccagat gtcgtgcccg cgccgtcctt gctgcgcgct tgccggcggt ctcgaccacg
      301 aaatcgatca ccggcccgat ggtgatggcc gctgccgcgg ccggaagcgc gccgggcacc
      361 gcggcctgat cgatgatgtt ggtcaggttg agaaccggat tgggcctttc gcgcgccgtc
      421 agctcggccg cctttgcggc tgccagacgg gcctgcgcga taggaatgtc gggatggaaa
      481 tacaaagcgg cgagcgtcag gcgggccaga tcccatgcgt ggcgcgacga cgcagaatcg
      541 tgccgcgcca gcgccgcctg tacgaaggtc aacaggcgcg ggtcgcgcag gctgcgggat
      601 tccagggctt gtgcattggc agcagccgaa agcggggcag gcttgtcgtg tgcgcagccg
      661 cacagcgacg ccgccagacc agccgccatg atcgcggccc gcgccatgga catgcggccg
      721 ccctcccttg gattttgccg gagtatagca gactggttct cgtcgcgtca tgacgagtcc
      781 cggcccggca cggagaggct ggggcttcag acctgaagcg gcgcggaatt gaaccgcgcg
      841 gcggcttggc atgccacagc gcgcgcggtt atagcctcac gccagggtaa tggggacgga
      901 gtgacgcgca aatggcaatt tctcccggct gggatattta gcatggatcg actgattgcc
      961 ggctatcacc gcttccgcca gtcgcgttgg ccggagcgca aggcgaccta tgaaagcctg
     1021 gccgaacacg gacaagcgcc gcgtgcgctg gtgctggcgt gttccgacag ccgggtggac
     1081 cccacgatga tcttcgacgc cgcaccgggc gagctattca tcgtgcgcaa tatcgccaat
     1141 ctggtgccgc cgtatcagcc ggacgcacgc tggcatggca ccagtgccgc gctggagttc
     1201 ggtgtccgtg tgctgcaggt gccggacctg atcgtgatgg gccacgcgat gtgcggcggg
     1261 atcaacgcat tgctgaatgg cgtgccgccg gaatgcggcg atttccttgc gagctggatc
     1321 aagattgccg aaccggcgaa gcgccgcgtt ctgcaatgcg ctccagccga cccgctgacg
     1381 gccgccgagc atgaggcggt gaagctctcg ctggagaacc tgctcagctt cccatggatc
     1441 gcggagcggg tgcaaagcgg caagctgact ctgcacggag cgatgtacgg catccgatcc
     1501 ggcgaactgt cgctgctggg agacgacggc gcattccacg cggtcccgac ggcctagccg
     1561 ggctgcgcgg gctcgggatg cgcggcgaac cacgccgcgt cctcgggctt ggcgacatag
     1621 cccggattgc ctggccaatc ccgggtgcgc gttgacatgt cgtcgccgta gcgcacgcgg
     1681 aacagcaccg tgcgctgcgg gccgttgcgg tactcgtgca gctcgccgcg cggatgcacg
     1741 acgaagcagc cgggtttcac gtccacggtc tgcgtcggcg tccacatcac gccgccgcct
     1801 tcatggcaga agtagatctc cgtcgcgttc gggtggagat ggttggggct ggtctgcccg
     1861 ggttcccagc aggcgatggt gacgtcgccc tcccctaccc ggcccagtac cttctccaca
     1921 tgcttgcgcg gatcgaacac ctgctccgcc gacagatcga acaccagcat gcgcgcctcc
     1981 caggtcaggc cagagtgaat ttcggcagcg tgatttcatc ggtggccggc tcgaacacca
     2041 cgcgcaccgg cgcaccgagt ttcgcgtttg cgtccgagcc cacaaggttg ctgaccagca
     2101 gcggcccttc ctccaactgc accaggcaca ccttgtacgg caggccgggc ttgaatccgt
     2161 cccagtaggc gcggtggaac tccacccagg actgcagcgt gccgcgtccg gacacgtcct
     2221 tccatgactg ctccggactc aggcatcgcg ggcataccgg tcctggcgga aaccgcgtat
     2281 cgccgcaggc cgtgcaatat tgcacgacaa ggcggttctg ccgggctgcc tcccagaacg
     2341 gctggttcag cgtgtcgatg cgcggcagcg gcttggcata gctcatgaga ttgctccttc
     2401 agccgcgcgt gtagatgatg gagcgcgtgc agggtgtcgc ctgcacgtat tgcaccacct
     2461 cgcagtcggg tacctggcgt gcgccgcatt cgccgcgcaa ctgccgcacc gcctcgacgt
     2521 tcagcgccca gccctgcatg taggagttgg acaggtgc
//
LOCUS       DASSZH010000434         2101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_759956, whole genome shotgun sequence.
ACCESSION   DASSZH010000434 DASSZH010000000
VERSION     DASSZH010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759956"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VFN42_09365"
     CDS             <1..270
                     /locus_tag="VFN42_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996864.1"
                     /translation="DLERIADFGLFGIGRGENVALNKALIEFATARPESRDATAKKLQ
                     AAITAYRSVIEGGLVDDIDNNPFGVSVNVRTTLGRALDEIERAVG"
     gene            complement(293..841)
                     /locus_tag="VFN42_09370"
     CDS             complement(293..841)
                     /locus_tag="VFN42_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996865.1"
                     /translation="MADITLAEFSGRVWLVGGEAHLHDLLANTLPPDISTELVQCERP
                     EDVHNLWVQLCGAQPTDGMPWQIHPNIVARIHRTCPDYAVYFAQWSAMLDTEALAVIN
                     AAGLQAHQNPDVPVVLAEYLDPEGPQAMADLSRLRAQLIEDKLVEHGVDRARITREKH
                     NVSEVPGMAQESQRVDIVVRAS"
     gene            911..>2101
                     /gene="tagH"
                     /locus_tag="VFN42_09375"
     CDS             911..>2101
                     /gene="tagH"
                     /locus_tag="VFN42_09375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HET8996866.1"
                     /translation="MAIVLTLTGPRGSGDHDTRTLRNGTLSIGRGPGNDWVLPDPERH
                     LSKAHCMIVGAGGHYLLTDISTNGVFVNGASERVPRNGQVELTDGDEVRLGDYVIMVS
                     EAADAAHAPVAAHEALVPGGVRRADPFAAPSDLTPDPLGGDPLNDPFGMPAPAGFVHP
                     IAAPPPALRPSDPFDLADEEKRAYRDPEEDLFKGVTPAEQWQGPSQADSADAPLHAFN
                     APRPIAVNNFDDLDIDALLGDTPPGQAPAGFTPAPTPRPAAPAPRQPAPPPPADFDDL
                     LGDTPPGAPAEPWGEHAAPPAPVPPPPAPPAPSAAPASPPPRAARPVADATPDTTPAA
                     PAPMPADAARLLAAFLEGAGVADLNLGPDPEKAMRAAGTVFQAMVEGVRQVLISRAAI
                     KNEFR"
BASE COUNT          327 a          801 c          670 g          303 t
ORIGIN      
        1 gacctggagc ggatcgccga tttcggcttg ttcggcatcg gccgcggaga aaacgtggcc
       61 ctcaacaagg cgctgatcga gtttgccacg gcacgcccgg aaagccgcga cgccacggcc
      121 aagaagctgc aggccgcgat caccgcctac cgttcggtga tcgagggcgg cctggtggac
      181 gatatcgaca acaatccctt cggcgtttcg gtgaacgtgc gcaccacgtt gggccgcgca
      241 ttggacgaga tcgaacgcgc ggtcggctag caggccgggc aggcgtcgtg cctcagctcg
      301 cgcggaccac gatgtccacc cgctggctct cctgcgccat gccaggcact tcgctcacgt
      361 tgtgcttctc ccgcgtgatg cgcgcgcgat ccacgccatg ctccaccagc ttgtcctcga
      421 tcagctgtgc ccgcagccgt gacagatcgg ccatcgcctg cggcccttcc ggatcgagat
      481 actcggccag caccacaggc acgtccgggt tctgatgcgc ctgcaggccg gcggcgttga
      541 tcacggccag cgcctcggta tccagcatgg cggaccactg cgcgaaatac accgcataat
      601 ccgggcaggt ccggtggatg cgcgccacga tgttggggtg gatctgccac ggcatgccgt
      661 ccgtcggctg cgcaccgcaa agctgcaccc acagattgtg aacatcttcc gggcgctcgc
      721 attgcaccag ttcggtgcta atgtcgggtg gcagcgtgtt ggccagcaga tcatggagat
      781 gcgcttcccc gcccaccagc caaacccgac cactgaattc ggccagcgtg atgtccgcca
      841 tgaaaacccc caagccgctt gtccctaata ccgcgtccgg acgcaccggc gccgagtccg
      901 gcggccctgc atggcaatag tcctgacatt gaccgggccg cgcggctctg gcgatcacga
      961 cacccgcacg ctgcgcaacg gaactctatc cattggccgg ggacccggca atgactgggt
     1021 cctgcccgat cccgagcggc atctgtccaa ggcccactgc atgattgtcg gtgccggcgg
     1081 ccattacctg ctcaccgaca tcagcaccaa cggcgtgttc gtcaacggcg cctccgagcg
     1141 ggtgccgcgc aacggccagg tcgagctgac cgatggcgac gaggtccggc tgggcgatta
     1201 cgtgatcatg gtcagcgagg cagccgacgc cgcgcatgcc cccgtggccg cacatgaggc
     1261 actggtcccg ggcggcgtgc ggcgcgccga tcccttcgcc gcgccctccg accttacccc
     1321 cgatccgctc ggcggcgatc cgctgaacga cccgttcggc atgccggctc cggcgggctt
     1381 cgtgcacccg atcgccgccc cgccgcccgc gttgcgtccc agcgacccgt tcgacctggc
     1441 ggatgaggag aagcgcgcgt accgggaccc cgaggaggac ctgttcaagg gcgtcacccc
     1501 ggccgaacaa tggcagggcc catcgcaggc cgacagcgcg gatgcgccgc tgcatgcctt
     1561 caatgcgccg cgcccgatcg cggtgaacaa tttcgacgac ctggacatcg acgccctgct
     1621 cggcgacacg ccgccgggtc aggcaccggc cgggttcacc ccggccccca cgccgcggcc
     1681 ggccgccccg gccccccggc agccagcgcc accgccgccg gcggatttcg acgatctgct
     1741 gggcgacacc ccgcccggag caccggccga accctggggg gagcacgcag cacccccggc
     1801 cccggtgccc ccgccacccg caccaccggc gccttccgcg gcacccgctt ctcccccgcc
     1861 gcgggccgcc cggcctgtgg cggacgccac gcccgacacc acccccgcgg cgcccgcgcc
     1921 gatgccggcg gatgcggcac ggctgctcgc cgccttcctg gaaggcgccg gcgtcgccga
     1981 cctcaacctc ggtcccgatc cggaaaaggc gatgcgtgcc gccggcacgg tgttccaggc
     2041 catggtcgag ggcgtgcgcc aggtgctgat ctcccgcgcg gccatcaaga acgagttccg
     2101 c
//
LOCUS       DASSZH010000435         7265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_283087, whole genome shotgun sequence.
ACCESSION   DASSZH010000435 DASSZH010000000
VERSION     DASSZH010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7265
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283087"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VFN42_09380"
     CDS             <1..588
                     /locus_tag="VFN42_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996867.1"
                     /translation="PVTNRFFFGSTDNQIVVVSGTGPNGIGNKLGTIAISGGTDEGAI
                     DVKARLAFFGSGAGVVDVVNLDTMKLVATLPAETGMHTLTVDTRTHELYVYENHRNVV
                     DAWHYKSDGHRMSFIAGPQKGAIGDSDGASGGTLTDLLRSANAAPPASPGAPNGDAGG
                     SAMVPDGPRLASSVLAGLDQIKTGNVLPPGFGHGT"
     gene            696..899
                     /locus_tag="VFN42_09385"
     CDS             696..899
                     /locus_tag="VFN42_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HET8996868.1"
                     /translation="MLTIDTEREDEGRWFAEITALPGVMAYGSTEAEARQKAAALAFR
                     VIAERLEHGEPVPDEARSLFVLA"
     gene            850..1221
                     /locus_tag="VFN42_09390"
     CDS             850..1221
                     /locus_tag="VFN42_09390"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HET8996869.1"
                     /translation="MGSQCRTRHAVCSCLPDGLARRQAGRGLRALRRIGWTIKRQSGS
                     HCTLARAGWPDHVFAFHDREEIGPRMLARIAKRTGLSAIRLLVRQLSEDSRNCALPSR
                     RKRYPYCSIPGVCSVGTFRDI"
     gene            complement(1620..1695)
                     /locus_tag="VFN42_09395"
     tRNA            complement(1620..1695)
                     /locus_tag="VFN42_09395"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1660..1662),aa:Lys,seq:ctt)
     gene            complement(1780..3489)
                     /locus_tag="VFN42_09400"
     CDS             complement(1780..3489)
                     /locus_tag="VFN42_09400"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HET8996870.1"
                     /translation="MDWDYIIVGGGSAGSVLANRLSARSANRVLLLEAGQDTPHGKVP
                     AEILDSYPGTAYLDPRFHWTELKVTTEVIPHNRPDAPKPPRRKYEQARVLGGGSSING
                     QLANRGAPTDYDEWEARGAAGWSWKDVLPFFKKVERDMDFQNDWHGTEGRIPVRRIFP
                     ELWPEHAKAVAESFRQAGYDYLIDQNAEFRDGYFPITISNLYERRVSAAIGYLDPGTR
                     LRENLTISTETQVSGLLFEGPRCVGVTAMVRGRPQEFRGREIILSSGAIHSPAHLLRA
                     GIGPAGHLRELGIPVRVNLAGVGQRLQDHPAVAVAAFLKPHARILHDWSRRHIYVALR
                     YSSGLPDIPAGDMLTVVTNKTSWHAVGEQIGSFIITVYKTYSESGQVRLASADWRDEP
                     EVAFNLLSDPRDLERLMDGVRRFGAMHLTPSMQAVTADPFPASYSDKVRQVGLINRKN
                     RWLTDAMARLLDGPPALRSWLMRRYIMEGLGLEDLLRDDDALEAFCRKAAVGVWHASC
                     TCRMGREDDPMAVTDPAGQVRGVDGLRVVDASIFPVVPCANTNFPVLMTAEKCAEAIL
                     AGL"
     gene            complement(3518..3889)
                     /locus_tag="VFN42_09405"
     CDS             complement(3518..3889)
                     /locus_tag="VFN42_09405"
                     /inference="COORDINATES: protein motif:HMM:NF024297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET8996871.1"
                     /translation="MASDLHEIEQTLQTYFDGLYEGDTGKLGRVFHDAAHLFSVAEGK
                     LTDWPRAQWFDMVRNRPSAQTQDLPRRDWVVQIDRSGPATAFAKVQCQIPPRYFTDYL
                     TLVKLADGWRIVSKTFHTETR"
     gene            3973..4416
                     /locus_tag="VFN42_09410"
     CDS             3973..4416
                     /locus_tag="VFN42_09410"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET8996872.1"
                     /translation="MRNSPAALYCISVVIQAYAMLDSLRIERTGVPIYVQIRDQVLRA
                     IGAGRLQPGERMPTMRQVAVALKIDLNTVRHAYDALARAGAIDVQPARGTFVAAQPPV
                     ADSAAHTQRLDDLAHRSIASARAAGLDPADLARRILELEQGKDVP"
     gene            4416..5309
                     /locus_tag="VFN42_09415"
     CDS             4416..5309
                     /locus_tag="VFN42_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HET8996873.1"
                     /translation="MRDLLFGRGSNAVAVLLSVILLLLAGACAWSGERWDAPAGYAAA
                     GILVVLAILAPRSLLIAAQWERMIVLRLGKLHGIRGPGPFIILPFIDSVATTIDQRIQ
                     TTAFTAERTLTRDTVPVNVDAIIFWQVHDTERAALEIANYREAISQVAQTSLREVIGA
                     TELSELLSERKQTDQLLRDEIGAKTAEWGVGIISVEIRDVAIPPGLQDAMSRRAQAER
                     EREARIILGASEQQVAQTFVDAAEIYARSPAAMQLRAMNLVYEATKERGMTIVVPSRL
                     ADSMDPSGMAGLLAAASASRA"
     gene            complement(5334..6431)
                     /locus_tag="VFN42_09420"
     CDS             complement(5334..6431)
                     /locus_tag="VFN42_09420"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET8996874.1"
                     /translation="MRGPLIIGGGPAGAAAACLLAQAGDPVTLLERQRGPADKVCGDF
                     LSAEAVEALQRLGIDLAALHPAPIHAVRLIHNARIAEAPLPFTALGLSRRALDEAILQ
                     RAVALGATVHRGCTVSGLAASKDGFAATCGGMGEIGSNSVFLATGKHDLRGLARRRPG
                     GGPVGLKMYYELVDNQRQQLAGAVELVLFPGGYAGLQAVEHGRAVLCLLMQADRFRQA
                     GGQWSVVLAALCAGASHLAQRLRGARAVLPRPLAVAGIPYGHLQPAAGPAGLYRLGDQ
                     ACVIPSLTGDGVAIALHSAAMAAAAWRGGQGASAYHALLARGLRRQMRTASLLHGLCL
                     APRLQSVAVMAAGLWPSTVGWGATATRVRSG"
     gene            complement(6416..7123)
                     /locus_tag="VFN42_09425"
     CDS             complement(6416..7123)
                     /locus_tag="VFN42_09425"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET8996875.1"
                     /translation="MSLAVRSSAGERMDTDCTGFADYQRCLRDLSRVNVVTRTYRPTL
                     AWLARETQGLAAFSLLDVGCGHGDMLRRIRRWARRRGIAARLEGIDLNPWSSRAAADA
                     TPADADIRYRTGDVFGFQPDLPFDFVISAQFAHHLDDAAVVGFIRWMEAHARRGWLIS
                     DLHRHRFAYHGFGVLARVAGWHRFVRHDGPVSIARGFLPRDLLALAADADLAPGAAAL
                     RRHVPFRLTLARRCAGR"
BASE COUNT         1246 a         2492 c         2383 g         1144 t
ORIGIN      
        1 cctgtaacca atcgcttctt ctttgggagc accgacaatc agattgtcgt cgtgtctggc
       61 actggcccca atggtatcgg caacaagctc ggcactatcg cgatcagcgg cggcaccgac
      121 gagggtgcca ttgatgtcaa ggcgcgtttg gcgttcttcg gcagcggcgc cggtgtcgtg
      181 gatgtggtca atctcgacac gatgaagctg gtagcgacgc taccagccga gacgggcatg
      241 cacacgctga ccgtcgatac gcgcacgcat gagctttatg tatacgaaaa ccaccgcaac
      301 gtcgtcgatg cgtggcacta taagagcgac ggccaccgga tgtcgtttat cgccggccct
      361 cagaaggggg cgattggcga cagcgacggg gcctcagggg gcacgctgac tgatttgctg
      421 cgatccgcca atgccgcgcc tccggcaagc cccggggcgc caaatgggga tgccggtggt
      481 tcggcaatgg tgccagatgg acctaggctc gcatcctccg tgcttgcggg gttggaccag
      541 atcaagacgg gcaatgtgct gccgcctggc tttggccacg gcacgtagcg ccgcgacctg
      601 gcagagaggg cgcgggcagc ggtcgcggcc acgacggggt tttcctctcg cgttgaccga
      661 ccaacggcgc catgttccag gtgcaggagg cagcaatgct gacgatcgat acggagcggg
      721 aagatgaagg ccgatggttt gcagagatca cggctctacc gggcgtgatg gcgtacggga
      781 gcaccgaagc cgaggcgcgg caaaaagccg ccgcgctcgc tttccgggtc attgccgaac
      841 gtcttgagca tggggagcca gtgccggacg aggcacgcag tctgttcgtg cttgcctgat
      901 ggattggccc gccgccaggc tggccggggc ctccgtgcgc tgcgtcgcat cggctggaca
      961 atcaagcgac agtccggttc tcattgtacg ctcgcgcgag ccggctggcc ggaccacgtg
     1021 tttgccttcc atgatcggga agaaattggg ccgcgcatgc tggcccgcat tgccaaacgc
     1081 accggacttt cggccatacg acttttagta cgacaattgt cggaggacag caggaattgt
     1141 gccctgccgt ctcgccgtaa gaggtatccc tattgcagta ttccaggggt atgctctgtc
     1201 ggaacattca gggacatttg atgcgccgcc gttgcctcat ttgatttgcc tcttcctccc
     1261 catggatgct gggtatctcg gcgcaagccg gcgcaccatc ctgaaccgac gtgtggactg
     1321 tttaatgcgg cccagcactg ggctgaacca gccgtcgaag ctgccgctct acttggccca
     1381 ggtctatgtg gaatggaaac agctcgataa acaggcggtg ctgtgggcca cgctccagtg
     1441 gttacgaaca gcgcgacatg caagctggcg tcgaggcact gctgagcctc tgtgccacgg
     1501 accccgctca ggcgctgtcg gaagcagaca aaatcgcctg gggtagcgtt gcgctggcca
     1561 tcgctcacct gcaagacgaa cagaacggag acctacgccc gaccccggca gacttctgtt
     1621 ggtgggcgca caaggactcg aaccttggac ccgctgatta agagtcagct gctctaccaa
     1681 ctgagctatg cgcccccacc gggcgttgtc ccccggtgcg ggcgcgctca tagcaagcgc
     1741 cgcccggaca tgcaaacccc ccctcgccgc cctgtcgcct tacaaccccg ccagaatcgc
     1801 ctcggcgcac ttctccgccg tcatcagcac cgggaaattc gtgttggcgc acgggacgac
     1861 cgggaaaatc gaggcatcca ccacccgcag cccgtccact ccccgcacct gcccggccgg
     1921 atcggtcacc gccatcgggt cgtcctcgcg tcccatccgg caggtgcagc tcgcatgcca
     1981 tacgccgacc gccgccttgc ggcagaatgc ctccagcgcg tcgtcatccc gcaacaggtc
     2041 ctccaggccg agcccctcca tgatgtagcg gcgcatcagc caggagcgga gtgccggcgg
     2101 accgtccaac agccgcgcca tcgcgtcggt cagccacctg ttcttgcggt tgatcaaacc
     2161 gacctggcga accttgtcgc tgtagctcgc cgggaacggg tcggcggtca ccgcctgcat
     2221 gctgggcgtc aggtgcatgg cgccgaagcg gcgcacgccg tccatcagcc gttccaggtc
     2281 gcgcgggtcg gacagcaggt tgaacgccac ctccggctcg tcgcgccagt cggcagaggc
     2341 cagcctcacc tgcccggatt cggaataggt cttgtagacg gtgatgatga aggagccgat
     2401 ctgctcgccc accgcatgcc aggacgtctt gttagtgacc accgtcagca tgtcgcccgc
     2461 cgggatatcc ggcaggccag acgagtagcg cagcgccacg tagatgtgcc ggcgcgacca
     2521 gtcgtgcagg atgcgggcgt gcggtttcag gaaggcggcg acggccaccg ccggatggtc
     2581 ctgcagccgc tggccgacgc cggccagatt gacgcgaacc gggatgccaa gctcgcgcag
     2641 atgcccggcg gggccaatac ccgcgcgcag cagatgcgcc ggcgaatgga tcgcgccaga
     2701 cgacaggatg atctcccgcc cgcggaactc ctgcggccgc ccgcgtacca tcgcggttac
     2761 gccgacgcag cgcggcccct cgaacagcag gccggagacc tgcgtttccg tggagatcgt
     2821 caggttctcg cgcagccgcg taccgggatc gagatagccg atcgccgccg acacccggcg
     2881 ttcgtacagg ttggagatgg tgatcgggaa atatccgtcg cggaattccg cgttctgatc
     2941 gatcaggtag tcgtagccgg cctgcctgaa gctctccgcc accgccttgg catgttccgg
     3001 ccacagctcg gggaagatcc ggcggaccgg aatgcggcct tccgtgccgt gccagtcgtt
     3061 ctggaaatcc atgtcgcgct cgaccttctt gaagaagggc agcacgtcct tccagctcca
     3121 cccggcggcg ccgcgtgcct cccattcgtc gtagtcagtc ggcgcgccgc gattggccaa
     3181 ctggccgttg atcgaggagc cgccgcccag cacgcgcgcc tgctcgtatt tgcggcgcgg
     3241 cggcttgggc gcatcgggcc ggttgtgcgg gatgacctcg gtggtgactt tcagctcggt
     3301 ccagtggaag cgcgggtcca ggtaggcggt gccgggataa gaatccagga tctccgccgg
     3361 caccttgccg tgcggcgtgt cctggcccgc ctccagcagc agaacacgat tggcgctacg
     3421 cgccgacagg cggttggcca gcaccgaccc ggccgaaccg ccaccgacga tgatgtaatc
     3481 ccagtccatc acccagcctc acagtcttca ggcaggatca gcgcgtctcg gtgtggaagg
     3541 tcttggagac gatgcgccag ccatcggcca gcttcaccag cgtcaggtag tcggtgaaat
     3601 agcgcggcgg aatctggcac tgcaccttgg cgaaagcggt ggccgggccg gagcggtcga
     3661 tctgcaccac ccagtcgcgc cgcggcaggt cctgggtctg cgcggaaggc cggttgcgca
     3721 ccatgtcgaa ccattgcgcg cgcggccagt ccgtcagctt gccctcggca acgctgaaca
     3781 ggtgcgccgc gtcatggaac acgcggccga gcttgccggt gtcgccttca tacaggccgt
     3841 cgaaataggt ctgcagggtc tgctcgatct cgtgcaggtc ggatgccatg gcgtcgtcct
     3901 ccgttctttc cgttcagcat cacgccaagc cgccacccgg gcaagccgcg gagagcggcc
     3961 gcgattttcc gcttgcgcaa ttctccggcc gcgctctact gtattagtgt tgtcatacag
     4021 gcctacgcca tgctggacag cctgcggatc gagcgaaccg gcgtaccgat ctacgtgcag
     4081 atccgtgacc aggtgctgcg cgcgatcggt gccgggcggc tgcaacccgg cgaacgcatg
     4141 ccgacgatgc gccaggtcgc ggttgcgctg aaaatcgatc tcaacacggt ccgccacgcc
     4201 tacgacgcgc tggcgcgcgc cggtgcgatc gacgtgcagc cggcccgcgg cacctttgtc
     4261 gccgcgcagc cgccagtcgc cgactctgcc gcacacacgc agcgcctgga cgacctggca
     4321 caccgcagca tcgcctcggc gcgcgccgcc ggactcgatc cggccgacct ggcgcgccgc
     4381 atcctggaac tcgaacaagg gaaggacgtg ccgtgatgag agacctgctg tttggccgcg
     4441 gcagcaatgc cgtcgcggtt ctgctgtccg tcatcctgct gctgctggcg ggcgcctgtg
     4501 cctggtccgg ggagcgctgg gacgcgccgg ccgggtatgc cgccgccggc atcctggtgg
     4561 tgctcgcgat cctggcgccc cgctccctgc tgatcgccgc gcaatgggaa cgcatgatcg
     4621 tgctgcggct gggcaagctg cacggcatcc gcggcccggg tcccttcatc atcctgccct
     4681 tcatcgacag cgtggccacc acgatcgacc agcgcatcca gaccacggcc tttaccgcag
     4741 aacgcacgct gacgcgcgac accgtgccgg tgaatgtcga tgcgatcatc ttctggcagg
     4801 tgcacgacac cgagcgtgcc gccctcgaga tcgccaatta ccgcgaagcc atcagccagg
     4861 tcgcgcagac ttcgctacgg gaggtgatcg gcgccaccga gctttccgaa ctcctgtcgg
     4921 agcgaaagca gaccgaccag ctgctgcgcg acgagatcgg tgccaagacg gcggaatggg
     4981 gcgtcggcat catctccgtg gaaatccgcg acgtggcgat cccgcctggg ctgcaggacg
     5041 cgatgagccg gcgcgcccag gcggaacgcg agcgggaagc acgcatcatc cttggagcat
     5101 cggagcagca ggtggcgcag accttcgttg acgcggcgga aatctacgcg cgctcgccgg
     5161 cggcgatgca gttgcgcgcg atgaacctgg tgtatgaggc aaccaaggaa cgcggcatga
     5221 ccatcgtggt gccgtcacgc ctggccgaca gcatggaccc gagcggcatg gcggggctgt
     5281 tggcggcggc aagcgcctcc cgcgcctgac cctctgtcat gcagccgccg ccgttacccg
     5341 ctccgcaccc ttgtggccgt cgccccccat ccaacggtgc tcggccacaa tccggcggcc
     5401 atgacggcca cgctctgcag ccgcggcgct aggcacagcc cgtgcagcaa ggaggccgtg
     5461 cgcatctgtc gccgcagccc gcgagccagc agcgcatgat aggcggaagc cccctgcccg
     5521 cccctccacg cggcagccgc catggccgca ctgtgcagcg cgatcgccac gccgtcaccg
     5581 gtcagcgacg gaatgacgca agcctggtcg cccagccggt acagtccggc tggaccggcg
     5641 gccggctgca aatggccata agggattccc gccacggcga ggggacgcgg cagaacggcg
     5701 cgtgcgccac gcagccgttg cgccagatgt gatgcaccgg cgcacagcgc cgccagcacc
     5761 acgctccatt gcccacctgc ttgccggaag cggtccgcct gcatcagcag gcacaacacg
     5821 gcccgtccgt gctcgaccgc ctgcaacccg gcgtagccgc ccgggaacag caccagttca
     5881 accgccccgg cgagttgctg ccgctgattg tccaccagct cgtagtacat tttcagccca
     5941 accggcccgc cgcctggccg gcggcgtgcg aggccgcgca ggtcatgctt gccagtggcg
     6001 agaaacacgc tgttgctgcc gatctctccc attccgccgc aagtcgcggc gaagccgtcc
     6061 ttcgatgccg ccaggccgct gacggtgcag ccacggtgca cggtggcgcc gagcgcgacg
     6121 gcccgctgca ggatcgcctc gtccaatgcg cggcgggaca agccaagcgc ggtgaagggc
     6181 agcggcgcct ccgcaatccg cgcgttgtgg atcagccgca cggcatggat cggcgccgga
     6241 tgcagcgcgg ccaggtcaat gcccagccgc tgcagcgctt cgacagcctc ggcgctgagg
     6301 aaatcgccac acactttgtc ggccgggccg cgctgccgct ccagaagcgt cacgggatcg
     6361 ccggcctgcg ccagcaggca tgcggccgcg gcgccagccg ggccaccgcc gatgatcagc
     6421 ggcccgcgca tcgcctggcc agcgtcaggc ggaagggcac gtgccggcgt agcgccgccg
     6481 ccccgggcgc caggtcagca tcggccgcca gggccaggag atcccgcggc aggaagccgc
     6541 gggcgatcga taccggcccg tcatgccgca cgaaccgatg ccatcccgcg acgcgcgcaa
     6601 gcacgccgaa gccgtgatag gcgaaccgat gtcgatgcag gtcgctgatc agccacccgc
     6661 ggcgcgcatg cgcctccatc cagcgaatga acccgacgac cgcggcatcg tccaggtgat
     6721 gcgcgaactg cgcgctgatc acgaagtcga acggcaaatc cggctggaat ccgaacacgt
     6781 cgccggttcg gtagcgtata tcggcgtccg ccggcgtggc atcggcggcg gcgcggctgc
     6841 tccagggatt gagatcgatg ccttccagcc gggcagcgat gccgcggcgg cgcgcccagc
     6901 ggcggatgcg gcgcagcatg tcgccatggc cgcaaccgac atccagcagg ctgaacgccg
     6961 ccaggccctg ggtttcccgc gccagccagg ccagcgtcgg gcgatacgtg cgggtgacga
     7021 cattcacccg cgacaggtcg cgcaggcaac gctgataatc ggcaaagccg gtgcaatcgg
     7081 tgtccatccg ctcgccggcc gaactgcgga cggcgagact catgcccgct ggaacgcgaa
     7141 ggtttccacc gacaggcctg gcccgaacgc catggcgatg ccttcgccct tggccggctg
     7201 gcgcagaatg cgatcgagca cgaacaccag cgtggcggag gacatgttgc cgaagttgcg
     7261 cagca
//
LOCUS       DASSZH010000436         3341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_862577, whole genome shotgun sequence.
ACCESSION   DASSZH010000436 DASSZH010000000
VERSION     DASSZH010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862577"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /gene="tolQ"
                     /locus_tag="VFN42_09430"
     CDS             complement(<1..629)
                     /gene="tolQ"
                     /locus_tag="VFN42_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HET8996876.1"
                     /translation="MDRAVDAVNLAAAGGDLSLWGLFMQADIVVKIVMFGLLAASVWV
                     WAVVFEKVTSLRKANKQADGFEDRFWSGGSLDDLFEQEGVNAGHPMAAVFSAAMGEWR
                     RTVRVAGADISHTSVRERIDRAITVTVQREMDRMERWMVFLASVGATAPFIGLFGTVW
                     GIMHSFSAIAAMHNTNLAVVAPGIAEALFATAIGLVAAIPSVLAYNKIST"
     gene            complement(662..1129)
                     /gene="ybgC"
                     /locus_tag="VFN42_09435"
     CDS             complement(662..1129)
                     /gene="ybgC"
                     /locus_tag="VFN42_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HET8996877.1"
                     /translation="MNQHHSVIPPEGKCRYTLRVYYEDTDAGGVVYHASYLRFAERAR
                     TEALRELGIPHSEMVERFAVMFLVRRAEIDYLRAARLDESLTVVTEALAVRGASVFLR
                     QDIQGTAGSCAVANLRLACVQVGGNKPERIPAHWRDTLQALCAARGAAGAAPA"
     gene            complement(1126..2178)
                     /gene="ruvB"
                     /locus_tag="VFN42_09440"
     CDS             complement(1126..2178)
                     /gene="ruvB"
                     /locus_tag="VFN42_09440"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014105299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET8996878.1"
                     /translation="MSDDRAVSPARLEDDAGEASLRPQTLAEFIGQRATRENLSVFIQ
                     AARARGEALDHVLLHGPPGLGKTTLAQIVARELGVGFRATSGPVIARAGDLAAILTNL
                     QPRDVLFIDEIHRLQPAIEEVLYPAMEDFQLDLIIGEGPSARSVRIDLPQFTLVGATT
                     RAGLLATPLRDRFGIPLRLVFYTPEELQIIVATGARKLGLDLTGDGAAEIARRSRGTP
                     RIAGRLLRRVRDFAAVEGSGPVDRAIADAALHRLEVDRLGLDAMDRRYLRRIAEHHAG
                     GPVGVETLAAALAEARDTLEDVVEPFLIQEGLVLRTSRGRMLGEPGWRHLNLAPPPAL
                     PAQLDLLRDPPLAEDE"
     gene            complement(2426..2653)
                     /locus_tag="VFN42_09445"
     CDS             complement(2426..2653)
                     /locus_tag="VFN42_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996879.1"
                     /translation="MSMLMIVGALLALFGIVGLAIPQFTTSHTKNVATVGPLHVQATE
                     HESHFVPPIVAGGALVLGIVLIGGGLMQRRP"
     gene            complement(2856..>3341)
                     /gene="ruvA"
                     /locus_tag="VFN42_09450"
     CDS             complement(2856..>3341)
                     /gene="ruvA"
                     /locus_tag="VFN42_09450"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET8996880.1"
                     /translation="AVTRLLIETHVREDAILLYGFADPAEREWFRLLNTVQGVGARVA
                     LSLLSALSPRDLIGAIAAGDRASITRAPGVGPRLAVRLLTELREKAGAMPSSAGTALP
                     ATRPAGVAEDALSAMVNLGYRRPEAQPVVVRVAERLGEQAGLDAVIRDSLKELAQRTG
                     G"
BASE COUNT          519 a         1175 c         1155 g          492 t
ORIGIN      
        1 gtgctgatct tgttgtaggc cagcaccgag ggaatcgccg cgaccaggcc gatcgcggtg
       61 gcgaacaacg cctcggcgat gccgggggcg acgacggcca ggttggtatt gtgcatggcg
      121 gcgatggccg agaacgagtg catgatgccc cacaccgtgc cgaacaagcc gatgaacggc
      181 gcggtggcgc cgaccgaggc gaggaacacc atccagcgct ccatccggtc catctcccgc
      241 tgcaccgtca cggtgatcgc ccggtcgatc cgttcgcgca cgctggtatg gctgatatcc
      301 gcgccggcga cgcggacagt gcggcgccat tcgcccatcg cggcgctgaa caccgccgcc
      361 attggatggc cggcgttgac gccttcctgc tcgaacaggt cgtccaggct gccgccggac
      421 cagaagcgat cctcgaaccc gtccgcctgc ttgttggcct tgcgcaggct ggtgactttc
      481 tcgaacacca ccgcccagac ccagacgctg gcggcgagca ggccgaacat cacgatcttc
      541 acgacgatgt cggcttgcat gaacaggccc cagagggaca ggtcgccacc cgccgccgcc
      601 aggttcaccg cgtcaaccgc acgatccact ccaaccccct agtctctggc cccgcggcca
      661 gtcatgccgg cgcagccccg gccgcacccc gtgccgcgca cagggcctgt aatgtgtccc
      721 gccaatgggc gggtatccgt tctggcttgt ttcccccgac ctgcacgcaa gccagccgca
      781 gattggcaac cgcgcaggag ccggccgtgc cctggatatc ctggcgtaag aacacggatg
      841 caccccgcac ggccagagcc tctgtcacga ccgtgagtga ttcgtcgagt cgcgccgcac
      901 gcagatagtc aatctccgcg cgccgcacca agaacatcac ggcgaaacgt tccaccatct
      961 cggagtgtgg gatgcccagc tcccgcagcg cctcggtccg ggcccgctcg gcgaagcgca
     1021 ggtaggaggc atggtagacc acgccgccgg catcggtgtc ctcataatag acgcgcagcg
     1081 tgtagcggca cttcccctcc ggcgggatga cggaatgatg ttggttcatt catcctccgc
     1141 caaaggcggg tcgcgcagca ggtcgagctg ggccggcagc gcgggcggcg gcgccaggtt
     1201 caggtggcgc cagcccggtt cgcccagcat gcggccccgg ctggtgcgca gcaccaggcc
     1261 ttcctggatc aggaacggct ccaccacgtc ttccagcgtg tcgcgcgcct ccgccagcgc
     1321 ggctgccagc gtctccacac ccacggggcc gccggcatgg tgttcggcga tgcgccggag
     1381 atagcggcgg tccatcgcgt ccaggccgag ccggtccacc tccagccgat gcagcgcggc
     1441 gtcggcaatg gcgcgatcca ccgggccgga gccctccacg gcggcaaagt cgcgcacccg
     1501 gcgcagcagc cggccggcga tgcgtggcgt tccgcgggaa cggcgggcga tttccgccgc
     1561 accgtccccg gtgaggtcca ggccgagctt gcgggcgccg gtggcgacga tgatctgcaa
     1621 ctcctcgggg gtgtagaaga ccaggcgcag cggaatgccg aaccggtcgc gcagcggggt
     1681 ggcgagcagg ccggcccgcg tggtggcgcc gaccagggtg aattgcggca ggtcgattcg
     1741 cacgctgcgt gcggacggcc cctcgccgat gatcaggtcg agctggaagt cctccatcgc
     1801 cggatacagc acctcctcga tggcgggctg cagccggtgg atttcgtcga tgaacaggac
     1861 atccctgggc tggaggtttg tcaggattgc ggcaaggtcg ccggcccggg cgatcaccgg
     1921 gccggaggtg gcgcggaacc cgacgcccag ttcgcgggca acgatctgcg ccagggtggt
     1981 cttgcccagc ccgggcggcc cgtgcagcag cacgtggtcc agcgcctcgc cgcgggcgcg
     2041 cgcggcctgg atgaacaccg acaggttctc ccgcgtcgcg cgctggccga taaattccgc
     2101 gagagtctgc gggcgaagcg atgcctcacc cgcatcgtcc tcgagccggg cgggggacac
     2161 ggcgcgatcg tcactcatcg ggtcggctct gctggcatgg tgaaattgtc cgcaccctgg
     2221 gtcatgctgc cgagtcggcg atgcattcgc agcgcagctt gtttagcgcg ttgcgggatt
     2281 cgggggcagg gcgcgcgtgt ctccgccgcg gggagccgca ttctgccacc cgggccggtg
     2341 cgcactgccg gcaaatatgg cgcaatcggc ctgccgcggt gatgcggcag cggtccccct
     2401 ccggccagcg gagggggcgc ctgggtcagg gccggcgctg catcagcccg ccgccgatca
     2461 gcacgatccc caggaccagc gcgccgccgg cgacgatcgg tggcacgaaa tgcgattcgt
     2521 gctccgtcgc ctgcacgtgc agcgggccaa ccgtggccac gtttttggtg tgtgacgtgg
     2581 tgaattgcgg gatcgccagg ccgacgatgc cgaacaatgc gagcagcgcg ccgacgatca
     2641 tcagcatgct cattgaagac cgcacaggaa cctccattgg tgtgcgcggc gaactgggaa
     2701 gccgacctac tgtgcgccag ccgtttcgct atcaacttac cgcaatgaac tctgcggtcg
     2761 gcggcccgcc tgggcggggt gccgcaccgg cggcggccat tggcgccggg tgcgcgggcg
     2821 gcggcagagc acctggcctg gcctgacctg tcgggtcagc cgccggttcg ctgcgccagt
     2881 tccttcaggc tgtcccggat caccgcgtcc agcccggcct gctcgcccag ccgttcggcg
     2941 acccgcacca ccacgggctg cgcttccggc cggcggtagc ccaggttcac catagcggat
     3001 agcgcatcct ctgccacccc ggccggccgc gtagcgggaa gagccgtacc ggcggaggaa
     3061 ggcatcgccc cggccttctc ccgcagctcg gtcagcagcc ggacggcaag ccgcgggccg
     3121 acgccgggtg cccgggtgat gctggcgcga tcgccggcgg cgatcgcccc gatcaggtcg
     3181 cgtggcgaca aggccgacag cagcgacagt gccacgcgcg ctcccacgcc ctgcacggtg
     3241 ttcagcaggc ggaaccactc ccgttcggcc gggtcggcaa acccgtacag caggatggca
     3301 tcctcccgga catgggtttc gatgagcagg cgggtgacgg c
//
LOCUS       DASSZH010000437         6255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_607222, whole genome shotgun sequence.
ACCESSION   DASSZH010000437 DASSZH010000000
VERSION     DASSZH010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607222"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..444
                     /locus_tag="VFN42_09455"
     CDS             37..444
                     /locus_tag="VFN42_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET8996881.1"
                     /translation="MISNEVSDGLAGILRTAIVELVRRDGPDLSARQLGVFLTCYLET
                     EAQTVRGLAAKLGVSKPAITRALDRLSEFDLVRRKTDPLDRRSVLVQRTATGMAFLRE
                     LRTILRDAAAAMHITAEPAGQNRQRSGEAVRAH"
     gene            565..1095
                     /locus_tag="VFN42_09460"
     CDS             565..1095
                     /locus_tag="VFN42_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET8996882.1"
                     /translation="MHDPALPTAWTRPRPEHWGTDRDACYAALIPELDALLAGEPDAV
                     ATTANAAAAIYHGLPALNWAGFYFLRGAELVLGPFQGRPACVRIPLGRGVCGTAAAER
                     RSILVPDVQAFPGHIACDVESRAELVVPLLAGDRLLGVLDLDSPVPGRFDPADQHGCE
                     QLAAVVVRHLGNSVLS"
     gene            1144..1467
                     /locus_tag="VFN42_09465"
     CDS             1144..1467
                     /locus_tag="VFN42_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HET8996883.1"
                     /translation="MLIDHRTYTLRPGTMAKQMALYEEYGLKPQTRHLGQPLAYLITE
                     SGEVNTYVHIWVYKDAADRAARRAAMAADPDWQTFLQKNAEAGYLIAQRNNLMTPASF
                     APINR"
     gene            complement(1526..3007)
                     /locus_tag="VFN42_09470"
     CDS             complement(1526..3007)
                     /locus_tag="VFN42_09470"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HET8996884.1"
                     /translation="MSVMRDIFSGTAWAVAARWIVRGIGVISMVVLARLLSPVDFGVV
                     AMAIFIVGLIEVLGDAGLALQIIRHPDPQCRELNTVFTLELLIGLTLCAVMILVAPIG
                     AAFFHEPRLTLVIRVMALRPILTGLQNPGIALFRKNMTFNKDFEFLVWNKVVSFFIVL
                     GLAITLRNYWALVIGTLAASVIATVQSYRIHPFRPRLQLSGVREIWGFSFWILVQNLF
                     GFLDLQMDRLVIGRVKSTVLMGYYTVAGDTAFAPVREVVMPVTRVLFPGVVRMAGDRG
                     TLGTSFNRVLAGVAIASLSISAGIALIAHDATTVFFGPRWTGLGSLLSILALSEGLSA
                     LSQPLYTTLNALGRPRVVAGLNIVRQVLLLATAIPAAIYAGVEAVALARVGVGVVTFV
                     LMMVLHARLLRIDAVRPWRSLGRPVVAALMMASIVLLVQAAAPNDPALRMVLSVATGA
                     AAFPAALLLLWQRAGRPAGIEADLLAVGPPLLHGLRRRAWQRS"
     gene            3239..3841
                     /locus_tag="VFN42_09475"
     CDS             3239..3841
                     /locus_tag="VFN42_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008850766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET8996885.1"
                     /translation="MSAAAGNPPDSTLARLAAAARDCRVCAAHLPLGPRPVFRVSESA
                     RLLIVGQAPGTKVHETGIPWNDASGDRLRGWLAIERDVFYDETRVAIVPMGLCYPGRL
                     PKGGDAPPRRECASLWRDPLVAAMPQIRLTLLVGSYALAHTLGPGAMTERVRGFRDLL
                     PRYFPLPHPSWRTAAWERRHAWFATELLPALRARVREALR"
     gene            complement(3844..4155)
                     /locus_tag="VFN42_09480"
     CDS             complement(3844..4155)
                     /locus_tag="VFN42_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996886.1"
                     /translation="MIAPRILAILSAILLVGSVAIATVGPPAAPLGQVLYLMDEGVVA
                     RLHLFVAGHTAEWAWSTMVVPFLLRPAWLIPASIGLILAGLSLSLSNRNQARRPHRRS
                     Q"
     gene            complement(4152..5078)
                     /gene="msrP"
                     /locus_tag="VFN42_09485"
     CDS             complement(4152..5078)
                     /gene="msrP"
                     /locus_tag="VFN42_09485"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HET8996887.1"
                     /translation="MYIIRRRGWEIPESQVTPEALVLNRRGLLAGAAGLGLAGIAGGA
                     RADTALKPDPEFPAGRALTAENYATTYNNYYEFSDSKNLWRAAQAFKMSPWSISIGGM
                     VKQPRTIGFDDLLKQVTLEERIYRHRCVEAWAMTVPWTGFPLSQLLKIAEPLASAKYI
                     VFESHQEKAVEPGLASFYPWPYIEGLAMDEAANDLAFISTGLYGKPLPPQNGGPIRLT
                     VPWKYGFKSAKALGKISFTDKRPVNFWQAIAPSEYGFWANVNPAVSHPRWSQAEERLI
                     GTNEVVPTQIYNGYGKWVAGLYVNRKNEKLFM"
     gene            5237..6232
                     /locus_tag="VFN42_09490"
     CDS             5237..6232
                     /locus_tag="VFN42_09490"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HET8996888.1"
                     /translation="MHLARFPRVRFFPAPTPLERLDNLTRQLGGPEIWIKRDDCTVVA
                     TGGNKVRKLEWLAGEALAQGADHLVTQGAVQSNHVRQTAAVARKLGMKCTALLEHRVE
                     TNDRDYLNSGNVLLDRLFDCAIEYRPPGLDMNAEAEAKGQALREAGGKPYVIPGGGSN
                     RVGALGYVSCAQELMQQADEMGLRIDRIVTATGSAGTHAGLVVGLQGCNANVPVLGIG
                     VRNPKDRQEAAVHKLAEATAEYVGVRGGIPRESVVANCDYVGPGYGQPTEGMKEAVRM
                     LASLEGILLDPVYSGKAMAGLIDLIRKGQFDKNERLVFLHTGGAVGLFGYTGVFE"
BASE COUNT         1031 a         2145 c         2116 g          963 t
ORIGIN      
        1 gcagcggtcc cactcctttc aggaaaggtc tagcgtatga ttagcaacga agtcagtgat
       61 ggtctggccg gcatcctgcg caccgcgatc gtggaactgg tgcgccgtga tggccccgac
      121 ctgtctgccc ggcaactcgg cgtcttcctg acctgctatc tggagaccga ggcccagacg
      181 gtgcgtgggc tggctgccaa gctcggcgtg tccaagccgg cgatcacccg ggcgctggac
      241 cggttgtcgg aattcgacct ggtgcggcgc aagaccgacc cgctggaccg gcgcagcgtc
      301 ctggtgcagc gcactgccac cggcatggcg ttcctgcgtg agctgcggac catcctgcgg
      361 gatgccgccg cggcgatgca catcacggcc gaaccggccg ggcagaatcg ccagcgctcc
      421 ggcgaggcgg tccgcgccca ctgaccggtt cgccgcgcct acgcccagac cacaccacag
      481 ccggcccgtg gctcggtgcc ggcaggtggt ccgcattgcc gacgccgcag ccctgggata
      541 tccgctgcgc cggaggtccg gcccatgcac gatcctgcct tacccaccgc ctggacccgg
      601 ccgcgccccg agcactgggg cacagaccgg gacgcatgct acgcggcgct gattccggaa
      661 ctcgacgccc tgctggccgg ggagccggat gcagtggcga ccacggcgaa cgccgcggcg
      721 gcgatctatc acgggctgcc ggcgctgaac tgggccggtt tctatttcct gcgcggggcg
      781 gaactggtgc ttggcccctt ccaaggccgt cccgcctgcg ttcgtatccc gctcggccgc
      841 ggcgtctgcg gcacggcggc ggcggagcgc cggagcatcc tggtgccgga cgtccaggcc
      901 ttccccggcc atattgcctg cgatgtggaa tcgcgcgccg aactggtcgt gccgctgctg
      961 gccggggaca ggctgttggg cgtgctggac ctggacagcc cggtgcccgg ccggttcgac
     1021 ccggccgatc agcacggctg cgagcagctc gccgccgtgg tggttcggca tctggggaat
     1081 tccgtgctta gctgaggtgg gccgcggatc gagcggctgc accccagggg gaggaactcc
     1141 agcatgctga tcgaccaccg cacctacacg ttgcgtcccg gcaccatggc caaacagatg
     1201 gcgctgtatg aggaatacgg actgaagccg cagacccgcc acctgggcca gccgctcgcc
     1261 tacctgatca ccgaatcagg tgaggtgaac acctacgtcc atatctgggt ctacaaggac
     1321 gccgccgacc gggcggctcg gcgcgccgcg atggcggccg atccggactg gcagaccttc
     1381 ctgcagaaga acgccgaggc cggctacctc atcgcccagc gcaacaatct gatgaccccc
     1441 gcctccttcg ctccgatcaa ccgctgaccg gcgcgggttg cctccctctc cgcccgcggg
     1501 gagggagtgc ggcgtccagg ctggttcagg agcgctgcca ggcgcgccgg cgcagcccat
     1561 gcagcagggg agggccgacc gccagcaggt cggcctcgat tcccgccggc cgcccggccc
     1621 gctgccacaa cagcagcagc gcggccggaa acgcggcggc gccggtggca acgctcagca
     1681 ccatccgcaa ggccggatcg ttcggggcgg ccgcctgcac cagcaggacg atgcttgcca
     1741 tcatcagcgc cgccaccacc gggcgtccca ggctgcgcca gggcctgacc gcatcgatgc
     1801 gcagcaggcg ggcgtgcagc accatcatca gcacgaaggt cactacgccc acgccgacgc
     1861 gggccagggc gaccgcttcg accccggcat agatcgcggc cggaatcgcg gtcgccagca
     1921 acagcacctg gcggacgatg ttcagcccgg cgaccacgcg cgggcggccc agcgcgttca
     1981 gcgtggtgta gagcggctgg ctgagcgcag agagtccctc cgacagcgcc aggatgctca
     2041 gcagcgatcc cagtccggtc cagcgcggac cgaagaacac cgtggtggcg tcgtgcgcga
     2101 tcagcgcgat gccggcgctg atcgacagcg acgcgatcgc cacccccgcc agcacccggt
     2161 tgaacgacgt tcccaacgta ccccgatcgc ccgccatgcg gaccacgccg gggaacagca
     2221 cgcgggtgac cggcatcacc acctcgcgca ccggtgcaaa ggcggtgtcg ccggcaacgg
     2281 tgtagtatcc catcagcacg gtcgacttga cgcgcccgat caccagccgg tccatctgca
     2341 gatccaggaa gccgaacagg ttctgcacca ggatccagaa cgagaatccc cagatctccc
     2401 gcactccgga cagttgcagg cgcggccgga agggatggat gcgatacgac tgcaccgtgg
     2461 cgatcacgct cgccgccagc gtgccgatca ccagcgccca gtagttgcgc agcgtgatgg
     2521 cgagtcccag cacgatgaag aacgacacga ccttgttcca caccaggaac tcgaaatcct
     2581 tgttgaaggt catgttcttg cggaacagcg caatgccggg attctgcaat ccggtcagga
     2641 tcggccgcag cgccatcacg cggatcacca gggtaagccg cggctcatgg aagaaggcgg
     2701 cgccgatggg cgcgaccaga atcatcaccg cgcacagcgt caggccgatc agcagctcca
     2761 gcgtgaagac ggtgttcagt tcacggcatt gcggatcggg atggcggatg atctgcaacg
     2821 ccaggccggc atcgcccagg acttcgatca gcccgacgat gaagatcgcc atggccacca
     2881 caccgaaatc caccggcgac aacaggcgtg ccagcaccac catgctgatg acgccgatgc
     2941 cgcggacgat ccagcgcgcc gccaccgccc atgccgttcc ggagaagatg tcgcgcatca
     3001 ccgacatcga cagcacccgc ccgtcattct ccccgcggtg cgcagcatgg ctgcaatttc
     3061 cttgtttggc ggccggagct gcatttgcac ccggagattt ccgcttcacc ccgttgtcac
     3121 attctgttga ctgattaaca cgttccggtg agtccgcata gccgctggac gcgcgggtgg
     3181 cgtctggcgg agaaacgtga tcgcgaaaac caggagtacg ccgctggccc gttcgattat
     3241 ttctgccgcc gccggaaatc cgccggacag cacgcttgcc cggctcgccg cggcggcgcg
     3301 cgactgccgc gtctgcgccg cgcatctgcc gctgggaccg cggccggtgt ttcgcgtctc
     3361 cgaatccgcc cgcctgctga tcgtcggcca ggcaccgggc acgaaggtgc atgagacggg
     3421 gatcccgtgg aacgatgcct ccggcgaccg gctgcgcggc tggctggcga tcgagcggga
     3481 cgtgttctac gacgagaccc gcgtggcgat cgtgccgatg gggctgtgtt atcccggccg
     3541 gctgccgaaa ggcggcgatg cgccgccgcg gcgcgaatgt gcgtcgctgt ggcgcgatcc
     3601 gctggtcgcc gccatgccgc agatccggct gaccctgctg gtgggcagct acgcgcttgc
     3661 ccacacgctg gggccgggcg cgatgaccga gcgggtgcgc ggtttccgtg atctcctgcc
     3721 gcggtatttc ccgctgccgc acccgtcctg gcggaccgcc gcctgggagc gcaggcacgc
     3781 ctggttcgcg accgaactac tcccggcgct gcgcgccagg gtacgcgagg cgcttcgcta
     3841 gtgctactgg ctgcggcggt gcggccgccg agcctggttg cggttggaga gggagagcga
     3901 caggccggcg aggatcagtc cgatcgaggc cgggatcagc caggccgggc gcagcaggaa
     3961 cggcaccacc atggtggacc atgcccactc cgccgtatgg ccggccacga acaggtgcaa
     4021 ccgcgcgacc accccttcgt ccatcaggta cagcacctga ccgagcggcg cggctggcgg
     4081 gccgactgtc gcaatcgcga ccgacccgac cagcaggatg gcagaaagga tggccaggat
     4141 gcgtggggct atcacatgaa cagcttttcg ttctttcgat taacgtagag gccggcgacc
     4201 cactttccat acccgttgta gatttgcgtt ggaacaactt cgttggtgcc gatcagccgc
     4261 tcctcggcct ggctccagcg cggatgcgac accgccgggt tcacgttcgc ccagaaaccg
     4321 tactcgctcg gcgcgatcgc ttgccagaaa ttgaccggcc gcttgtcggt gaagctgatc
     4381 ttgcccagcg ccttggccga tttgaacccg tatttccacg gcaccgtcag ccggatcggc
     4441 ccgccattct gcggcgggag cggcttgccg tacagcccgg tggagatgaa ggccaggtcg
     4501 ttggctgcct cgtccatcgc caggccctcg atgtacggcc agggatagaa ggaagcgagg
     4561 cccggttcga ccgccttctc ctgatgggac tcgaacacga tgtacttcgc cgaggccagc
     4621 ggttcggcga tcttcagcaa ctggctgagc gggaagccgg tccacggcac ggtcatcgcc
     4681 caggcttcca cgcagcggtg ccggtagatc cgctcctcca gcgtcacctg cttcagcagg
     4741 tcgtcgaagc cgatggtgcg cggctgcttg accatgccgc cgatgctgat gctccagggc
     4801 gacatcttga acgcctgcgc ggcacgccag aggttcttgc tatcgctgaa ctcgtagtag
     4861 ttgttgtagg tggtggcgta gttttccgcg gtcagcgcgc gtccggccgg aaattccgga
     4921 tcgggcttca gcgccgtgtc ggcccgcgcg cctcccgcga tgccggcgag gcccagcccg
     4981 gccgcgccgg ccagcaaacc acgccggttc agcaccagcg cctccggcgt gacctggctt
     5041 tccgggatct cccagccgcg gcgtcggatg atgtacattg ctcgcactcc ccattaagtc
     5101 gaacgaacgg cccgcgtgcc gccggtcacc cgcacgtgac gggaaactgc ggcgcgtccg
     5161 cccgcattgt cggacgcggc tcgatgggga acattacacc tgctccgcgc ttggggcaga
     5221 atttttggag gctccgatgc atctcgcccg tttcccccgg gtccgtttct tcccggctcc
     5281 cacgccgctg gagcggctgg acaatctgac gcgccagctc ggcgggccgg aaatctggat
     5341 caagcgcgac gattgcacgg tggtggccac cggcggcaac aaggtgcgca agctggaatg
     5401 gctggccggc gaggcgctgg cgcagggtgc cgatcatctg gtgacccagg gcgcggtaca
     5461 atccaaccac gtccgccaga ccgccgccgt cgcccgcaag ctgggcatga agtgcaccgc
     5521 gctgctggag caccgggtgg agaccaatga ccgcgactac ctgaacagcg gcaacgtgct
     5581 gctggaccgg ctgttcgatt gcgccatcga gtaccgcccg cccgggctgg acatgaacgc
     5641 tgaggccgag gcgaagggcc aggcgctgcg cgaggccggc ggcaagccgt acgtcatccc
     5701 gggtggcggc tccaaccggg tgggggcgct gggctacgtg tcctgcgccc aggagctgat
     5761 gcaacaggcc gacgagatgg ggctgcgcat cgaccgcatc gtcaccgcga ccggcagtgc
     5821 ggggacgcat gccgggctgg tggtcgggct gcagggctgc aatgccaacg tgccggtgct
     5881 gggcatcggc gtgcgtaatc cgaaggaccg gcaggaagcg gcggtgcaca agctggccga
     5941 ggccaccgcc gagtatgtcg gcgtgcgcgg cggcattccc cgcgagtcgg tggtcgcgaa
     6001 ttgcgactat gtcggcccgg gctacggcca gccgaccgag ggcatgaagg aagccgtgcg
     6061 catgctggcg agcctggagg gcatcctgct ggacccggtg tattccggca aggcgatggc
     6121 cgggctgatc gacctgatcc gcaagggcca gttcgacaag aacgaacggc tggtgttcct
     6181 gcacaccggc ggcgccgttg gactgttcgg ctacaccggg gtgttcgagt aatccctgcc
     6241 gggcggcggc gtggg
//
LOCUS       DASSZH010000438         3839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_692067, whole genome shotgun sequence.
ACCESSION   DASSZH010000438 DASSZH010000000
VERSION     DASSZH010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692067"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="VFN42_09495"
     CDS             complement(<1..895)
                     /locus_tag="VFN42_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET8996889.1"
                     /translation="MGAYLIRRLLLVIPTLFGIIAINFAIVQFAPGGPVEQMIAELKG
                     KGGGMTERVAGGGAEVAKPIVGENSVYRGRRGLDPVLVDDIRKMFGFDKPPLTRFWLM
                     LKGYLTFDFGRSFFRDRSVLSLILQKMPVSISLGLWSTLLVYAISIPLGVMKAVRDGT
                     RFDLVSSLVVLVGYAVPGFLFAVLLVVLFAGGSYVQWFPLRGLVSEGSQDWSWPARIA
                     DYLWHMVLPIASLVVGGFATLTILTKNCFLEEIRKQYVVTARAKGAGEQRVLYRHVFR
                     NAMLLIVAGFPSAFIGILFTSA"
     gene            complement(896..2770)
                     /locus_tag="VFN42_09500"
     CDS             complement(896..2770)
                     /locus_tag="VFN42_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8996890.1"
                     /translation="MRRRDLMLAGLAGLAAGIPKVRADEAPAQAAPAAEGEGAPLRTY
                     GLSLVGTPRLPEGFPYFPYVNPNAPKGGAVALADLGSFDSFNPFIVRGTAPQSIGRVW
                     DTLLRETADELDTGYAHLAAVIEIAADHSYVAFELRPEARFHDGKAVTAEDVVWTFEA
                     LRTKGRPYYRQYYAGVDKAVAEGERRVVFHFKRNDNRELPMILGELVVLPKHWWEGRD
                     FSAPLTDPPLGSGPYRVGAYEFGRTLTLQRVPEYWGRDLPTAKGQDNFGSIRTEYFRD
                     STVALQAFKAGQVDYRRENISKVWATEYEFPAVKKGLVRKQAFPSSLPTGMQCFVMNT
                     RRPVFADRRVREAMTQVFDFQWENKNLFYGLYTRTTSFFSNSGCASSGLPGPEELRLL
                     EPYRDKLPPEVFTKDFTLPVTDGSGNNREGMTRALKLMESAGYHVKSRKMIGPDGNQM
                     AFDILLDEPVFERVTLPYVQWLDHLGIRVNVRTVDPAEYQHLTDAFDFDMTINTYGES
                     DSPGNELVDFWGSAAAKQEGSDNLAGVSDPVVDALIAKVVGARTRDDLVTAARALDRV
                     LLWGWYVVPQWHIQSVWVAWWDRFGHPDVKVRSGVAFNTWWVDEELAEKTDAARRSGL
                     "
     gene            complement(2828..3628)
                     /locus_tag="VFN42_09505"
     CDS             complement(2828..3628)
                     /locus_tag="VFN42_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET8996891.1"
                     /translation="MTAAPAPEQVASWLAAAEAAVEAAGAVVRPLFRAGLTADLKSDR
                     SPVTEADRAAEQAMRAVIAHHCPGHGIIGEEFGETRPDARFRWALDPIDGTRAFITGR
                     PLFGTLVGLLDRGVPVLGVIDQPVTGERWLAAPGRPTSFRGPFGGRAGGRSCKTLDRA
                     ELSCTSPLMFAPDEFARWQRLAAACGRASFGGDCYGYGLLALGQIDVIAEADLKVWDW
                     AALVPVIEGAGGRMTDWQGRALREGGDGRALAVGDPALLAPALQFLGG"
     gene            complement(3636..>3839)
                     /locus_tag="VFN42_09510"
     CDS             complement(3636..>3839)
                     /locus_tag="VFN42_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HET8996892.1"
                     /translation="FNYSSALKSKKGPWTYEALNEWLTKPAAYAPGTMMGFPGLKSEQ
                     ERAEIIAYLRTLSDQPEPLPAAK"
BASE COUNT          621 a         1406 c         1237 g          575 t
ORIGIN      
        1 gcgccgaggt gaacaggatg ccgatgaagg cggagggaaa cccggccacg atcagcagca
       61 tcgcgttgcg gaacacgtgg cggtacagca cgcgctgttc ccccgccccc ttggcccgcg
      121 ccgtcaccac gtattgcttg cggatttcct ccaggaagca gttcttggtc aggatggtca
      181 gcgtggcgaa cccgcccacc accagcgagg cgatcggcag caccatgtgc cagagataat
      241 cggcaatccg cgctggccac gaccagtcct gcgagccttc gctgaccagc ccgcgcagcg
      301 ggaaccactg cacgtagctg ccgccggcga acagcaccac cagcagcacg gcgaacagga
      361 agcccggcac ggcatagccg accagcacga cgaggctgga gacgagatcg aagcgggtgc
      421 cgtcgcgcac cgccttcatc acgccgagcg ggatcgagat ggcatagacc agcagcgtgg
      481 accacaggcc gagcgagatg ctgaccggca tcttctgcag gatcagcgac agcacgctcc
      541 tgtcgcggaa gaagctgcgg ccgaagtcga aggtcaggta gcccttcagc atcagccaga
      601 accgcgtcag cggcggcttg tcgaagccga acatcttgcg gatgtcgtcg accagcacgg
      661 gatcgaggcc gcggcggccg cgatagacgc tgttctcgcc gacgatcggc ttggcgacct
      721 ccgccccgcc gccggcgacg cgctcggtca tgccgccgcc cttgcccttc agttcggcga
      781 tcatctgctc caccggcccg cccggcgcga actgcacgat cgcgaagttg atggcgatga
      841 tcccgaacag cgtcggaatc accagcagca accggcggat cagataggcg cccatctaca
      901 acccgctgcg ccgcgccgcg tcggtcttct cggcgagttc ctcgtccacc caccacgtgt
      961 tgaaggcaac cccgctgcgc accttcacgt cgggatggcc gaagcggtcc caccacgcga
     1021 cccagaccga ctggatgtgc cattgcggca ccacgtacca gccccacagc agcacgcggt
     1081 ccagcgcccg cgccgccgtc accaggtcat cgcgcgtgcg cgcgccgacc accttcgcga
     1141 tcagcgcatc gaccaccgga tcggacaccc cggcgaggtt gtcgctgccc tcctgcttcg
     1201 ccgccgcact gccccagaaa tccaccagct cgttgcccgg actgtcggat tcgccatagg
     1261 tgttgatcgt catgtcgaag tcgaaggcat cggtcaggtg ctggtactcg gccggatcga
     1321 ccgtgcgcac gttcacccgg atgccgagat ggtcgagcca ctgcacgtac ggcagcgtca
     1381 cccgctcgaa caccggctca tcgagcagga tgtcgaacgc catctggttg ccgtccgggc
     1441 cgatcatctt gcgactcttc acgtgatagc cggcggattc catcagcttc agcgcccgcg
     1501 tcatgccctc gcggttgttg cctgatccgt cggtcaccgg cagcgtgaag tccttggtga
     1561 acacctccgg gggcagcttg tcgcgataag gctccagcag cctgagttcc tccggccccg
     1621 gcaggccgga ggaggcgcag ccgctgttgc tgaagaaact cgtcgtgcgc gtgtagagac
     1681 cgtagaaaag gttcttgttc tcccactgga aatcgaacac ctgcgtcatc gcctcgcgca
     1741 cgcggcggtc ggcgaacacg gggcggcgcg tgttcatcac gaagcactgc atccccgtgg
     1801 gcaggctgga cgggaaggcc tgcttcctca ccagcccttt cttcaccgcc gggaattcat
     1861 attccgtcgc ccacaccttg gagatgttct cgcgccggta gtccacctgc ccggccttga
     1921 acgcctgcag cgccaccgtc gagtcgcgga aatactcggt gcggatgctg ccgaaattgt
     1981 cctgcccctt ggcggtcggc aggtcgcgcc cccagtattc cggcacgcgc tgcagcgtca
     2041 gggtgcggcc gaactcgtag gcgccgacac gatacgggcc ggaaccgagc ggcgggtcgg
     2101 tcagcggcgc ggagaaatcc cgcccctccc accagtgctt cggcagcacc accagctcgc
     2161 ccaggatcat cggcagttcg cggttgtcgt tccgcttgaa gtggaacacc acgcggcgtt
     2221 ccccctccgc caccgccttg tccacgccgg catagtactg gcggtaatag gggcggccct
     2281 tggtgcgcag cgcctcgaac gtccacacca cgtcctcggc cgtgaccgcc ttgccgtcgt
     2341 ggaacctcgc ctcggggcgc aactcgaagg cgacgtagct gtgatcggcg gcgatctcga
     2401 tcacggcggc cagatgggca tagccggtgt ccagctcgtc cgccgtctcg cgcagcagcg
     2461 tgtcccacac gcggccgatg gattgcggcg cggtgccgcg cacgatgaac gggttgaagc
     2521 tgtcgaagct gccgagatcg gcgagcgcca ctgcgccccc tttcggcgcg ttcggattga
     2581 cgtagggaaa atacggaaac ccttccggca gccgcggagt gccgaccagc gacaggccgt
     2641 aggtgcgcaa cggcgccccc tcgccctcgg cggcgggcgc cgcctgtgcc ggcgcctcgt
     2701 cggcgcggac tttcgggatg ccggcggcaa gcccggccaa tccggcaagc atcaagtccc
     2761 ggcgacgcat ctggttctcc tcccgcggcc tcaactaggt ataggcggtt cccgcgggta
     2821 cgtcaggtca cccccccaga aactgcaacg cgggtgccag cagcgccggg tcgccgaccg
     2881 caagcgcccg cccgtcgccg ccttcgcgca acgcacgacc ctgccagtcg gtcatccgcc
     2941 cgcccgcgcc ctcgatcacc ggcaccagcg ccgcccagtc ccagaccttc aggtcggcct
     3001 cggcgatcac gtcgatctgg ccgagcgcga gcaggccgta gccgtagcag tcgccgccga
     3061 aactcgcccg cccgcacgca gcggccaggc gctgccagcg cgcgaactcg tccggcgcga
     3121 acatcagcgg gctggtgcag gacagttcgg cgcggtcgag cgtcttgcag gaccgtccgc
     3181 ccgcgcgccc gccgaacggg ccgcggaacg acgtgggccg gcccggcgcg gccagccacc
     3241 gctccccggt caccggctgg tcgatcacgc ccagcaccgg cacgccccgg tccagcagcc
     3301 cgaccagcgt gccgaacagc ggccggccgg tgatgaaggc gcgggtgccg tcgatcgggt
     3361 cgagcgccca gcggaaccgc gcatcgggcc gggtctcgcc gaattcctcg ccgatgatgc
     3421 cgtgtccggg acaatggtgc gcgatcaccg cgcgcatcgc ctgttccgcc gcccggtcgg
     3481 cctcggtcac cgggctgcgg tcgcttttca ggtcggccgt cagcccggcg cggaacagcg
     3541 ggcgcaccac cgcgcccgcc gcctccaccg ccgcctcggc ggcggcgagc caggacgcga
     3601 cctgctccgg cgccggcgcg gcggtcacgc cgcgactact tcgcggccgg cagcggctcc
     3661 ggctggtcgg agagggtccg cagataggcg atgatctccg cccgctcctg ctcgctcttg
     3721 aggccgggga agcccatcat cgtgcccggc gcgtaggcgg cgggcttggt cagccactcg
     3781 ttcagcgcct cgtaggtcca gggccccttc ttgctcttga gcgcgctgga gtagttgaa
//
LOCUS       DASSZH010000439         3527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_896965, whole genome shotgun sequence.
ACCESSION   DASSZH010000439 DASSZH010000000
VERSION     DASSZH010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896965"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VFN42_09515"
     CDS             <1..511
                     /locus_tag="VFN42_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8996893.1"
                     /translation="GTGRILIRHRGELLDIAAADPRTVLAVRQETLGYVSQFLRVVPR
                     VAALDVVAEVLIARGTAPQQAHEAATALLQQLNIPRRLHGIPPATFSGGEQQRVNLAR
                     GFIADYPVLLLDEPTASLDAENRAVVVHMIRQAKARGTAIVAICHDSDVRDAIADRLF
                     ALSPLQDAA"
     gene            508..1644
                     /locus_tag="VFN42_09520"
     CDS             508..1644
                     /locus_tag="VFN42_09520"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HET8996894.1"
                     /translation="MTPEIILTNATLVLPDASLFGTLTIRGDRIADVQPGRAGQSAAI
                     DLHGDYLIPGVVDLHTDNLERQVQPRSLARWPSRSAMVAHDAQCAAAGVTTVLDALCL
                     GDLGFDRGRIRTFQDGVTDLDALTAAALLKSDHFLHLRCEVPALDMLGLFDSVADHRL
                     VRMISLMDHSPGVGQYADIDFYRALRRRGGLDDAYIERRISELQDQRAQHREPNRRAL
                     LDRVAGRGIALASHDDRTEQEIAENARDGITISEFPVTMLAAQAAKRAGMRVIAGAPN
                     IVRGGSHSGNVSAADLLQAEAVDAFASDYVPNSLVEAAFQCARNGLKLHDAVAMVTEH
                     PARMAGLADRGRLEAGQRADLVRVRLHEDLPVVRQVWRAGERVI"
     gene            1644..2318
                     /locus_tag="VFN42_09525"
     CDS             1644..2318
                     /locus_tag="VFN42_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1045 domain-containing protein"
                     /protein_id="HET8996895.1"
                     /translation="MQRVAIYYAPQADDPLFAASSCWLGRDPVSDMRAPQPDLPNIEE
                     VTAEPRLYGFHATLKPPFRLAEGRHWDDMLQAARALADRIAPFELPPLAVSDVHGFLA
                     LRETEPSAPLQALADACVAELDAFRAPPSAAELARRRRNGLAPQQDAMLVRWGYPYVF
                     ETWFFHMTLTRRLTAAEKLLFQPEAERWFSRALLLPRRVADLCLFVQPAPGASFVIQE
                     RLPLRG"
     gene            complement(2287..2832)
                     /gene="phnN"
                     /locus_tag="VFN42_09530"
     CDS             complement(2287..2832)
                     /gene="phnN"
                     /locus_tag="VFN42_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism
                     protein/1,5-bisphosphokinase (PRPP-forming) PhnN"
                     /protein_id="HET8996896.1"
                     /translation="MLVLVVGPSGAGKDTLLDAARRTLAGDPRVRFVRRVITRPADAG
                     GEDHEAVSDAAFAARDFALQWQAHGLRYGIPVEIAEDLAQGRTVVANVSRTVIAEAAR
                     RFPVQVIEVTAPPEVLAARLATRGREPAADAAARMARNVPLPEGVPLIRVLNDGSIAQ
                     GAERFVATLRGIIPAGAAAPG"
     gene            2906..>3527
                     /locus_tag="VFN42_09535"
     CDS             2906..>3527
                     /locus_tag="VFN42_09535"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET8996897.1"
                     /translation="MFTTRPEIAGTFGVVASTHWIASQVGMAVLERGGNAFDAAAATA
                     FTLQIAEPHLNGPGGDVPIILHSASSKTQHVICGQGVSPAAATLEKFRELDLDLIPAT
                     GLLPAVVPGAFDAWCLLLRDWGSWELPDVLSFAIDYARNGIHIVPRISAQIESVRPLF
                     EQEWKTSAAVFLPGGKVPAPESLFARPAVADTYERICKEAVGDTREA"
BASE COUNT          546 a         1293 c         1155 g          533 t
ORIGIN      
        1 cggcaccggc cgaatcctga tccgccatcg cggcgaactg ctcgacatcg ccgccgccga
       61 cccgcgcacc gtgctggcgg tgcggcagga aaccctcggc tatgtcagcc agttcctgcg
      121 cgtggtgccg cgtgtcgccg cgctggacgt ggtggctgag gtgctgatcg ctcgcggcac
      181 cgccccgcaa caggcgcatg aggccgccac cgcattgctg cagcaactca acatcccgcg
      241 ccggttgcac ggcattccgc ccgccacctt ctcgggcggc gagcagcaac gggtgaacct
      301 tgcccgcggc ttcatcgccg attacccggt gctgctgctg gacgaaccca ccgcctcact
      361 ggacgcggaa aaccgcgccg tggtggtaca catgatccgg caggcgaagg cacgcggcac
      421 cgcgatcgtg gcgatctgcc acgactccga cgtacgtgat gcgatcgccg atcgcctgtt
      481 cgccctgtcc cccctacaag acgccgcatg acccccgaaa ttatcctgac caatgcgacc
      541 ctcgtgctgc cggacgccag cctgttcggc acactcacca tacgtggcga ccgcatcgcc
      601 gatgtgcagc ccgggcgcgc gggacaatcc gccgccatcg acctgcatgg cgattacctg
      661 atccccggcg tggtcgacct gcacaccgac aatctggaac gccaggtgca gccgcggtcg
      721 ctggcgcgct ggccgtcgcg ctccgcgatg gtcgcgcatg atgcgcaatg cgccgcggcc
      781 ggtgtcacca cggtgctgga tgcgctgtgc ctgggcgatc tgggctttga ccggggacgc
      841 atccgcacct tccaggacgg cgtgactgat ctggacgcgc tgaccgcggc ggcgctgctg
      901 aaatccgacc acttcctgca tctgcgctgc gaagtgccgg cgctggacat gctgggcctg
      961 ttcgactccg tggctgacca caggctggta cgcatgatca gcctgatgga ccacagcccc
     1021 ggcgtcggcc agtacgccga tatcgacttc taccgcgcac tgcgccgccg cggcggcctg
     1081 gacgacgcct atatcgaacg ccgcattagc gaattgcagg accagcgcgc gcaacatcgc
     1141 gagccgaacc ggcgcgccct gctggaccgc gttgccggcc gcggcatcgc gctggcaagc
     1201 catgacgacc gcaccgagca ggagatcgcc gaaaacgccc gcgacggcat caccatcagt
     1261 gagttccccg ttacgatgct ggccgcgcaa gcggccaagc gcgccggcat gcgtgtgatt
     1321 gccggcgcgc ccaacatcgt gcgtggcggc tcacattccg gcaacgtctc cgccgccgac
     1381 ctgctgcagg ccgaagcagt ggatgccttt gcctcggact acgtgccgaa cagcctggtg
     1441 gaagccgcgt tccagtgcgc gcgcaacggc ctgaagctgc acgacgcggt cgctatggtg
     1501 acggagcatc ccgcccgcat ggccggcctc gcggatcgcg gccggctgga agcaggacaa
     1561 cgcgccgacc tggtccgcgt tcgcctgcat gaggatctgc cggtggttcg gcaggtctgg
     1621 cgtgccggcg aaagggtgat ctgatgcagc gcgtcgcgat ctattacgcg ccgcaagccg
     1681 acgatccgct gtttgccgcg tcgtcctgct ggcttggccg cgacccggtc agcgacatgc
     1741 gggcgccgca gccggacctg ccgaacattg aggaggtcac cgccgaaccg cggctgtacg
     1801 gtttccatgc cacgctaaaa ccgccgttcc ggctggcgga aggccgccac tgggacgaca
     1861 tgctgcaagc agcacgcgcc ctggcggacc gcatcgcgcc cttcgaattg ccaccactgg
     1921 cggtgtccga cgtgcacggg tttctcgcgc tgcgcgaaac cgaacccagc gcgccgctgc
     1981 aggcgctggc ggatgcctgc gtggcagaac tcgatgcgtt ccgtgcgccg ccatctgcag
     2041 cagaattggc ccgccggcgt cgcaacggcc tggcgccgca acaggacgcg atgctggtgc
     2101 gctggggcta tccctacgtg ttcgagacgt ggttcttcca catgacgctc acccgccggc
     2161 tgaccgcggc ggaaaagctg ctattccaac cggaagcgga acgctggttc agccgcgcgc
     2221 tgctgctgcc gcgcagggtc gcggatctct gcctgttcgt gcagcctgcc cccggcgcca
     2281 gcttcgtcat ccaggagcgg ctgcccctgc ggggatgatc ccgcgcagcg tggcaacgaa
     2341 ccgctccgcg ccctgcgcga tgctgccgtc gttcagcacc ctgatcagcg gcacgccctc
     2401 aggcagcggg acgttgcgcg ccatccgagc cgccgcatcg gccgccggct cccgaccccg
     2461 cgtggcgagc cgcgcggcca ggacctctgg cggcgcggtg acttcgatca cctgtaccgg
     2521 gaaccgccgc gcggcttcgg cgatcaccgt gcgggagacg ttcgccacga cggtgcggcc
     2581 ctgcgccagg tcctcggcga tctcgacggg gataccgtag cgcaggccgt gcgcctgcca
     2641 ctgcagcgcg aagtcgcgcg ccgcgaacgc ggcatccgag accgcttcat ggtcctcccc
     2701 gccagcatcc gccggccggg tgatcacccg gcgcacgaag cgcacccgcg gatcccccgc
     2761 cagcgtccgc cgtgcggcgt ccagcagcgt gtccttgccc gcgccgctcg gcccgacgac
     2821 cagcaccagc atgcgcaccc ctttgcattg acgcgccgca ccctgccgct agcctgcggc
     2881 cgaagcaatg caggagtcgc gcggcatgtt caccaccagg ccggaaatcg ccggtacctt
     2941 cggagtcgtt gccagcacgc attggatcgc cagccaggtc ggcatggcgg tgctggagcg
     3001 cggcggcaat gcgttcgacg ccgccgccgc caccgccttc accctgcaga tcgccgaacc
     3061 gcatctgaac gggccgggcg gcgatgtgcc gatcatcctc cacagcgcaa gcagcaagac
     3121 ccagcacgtg atctgtggcc agggcgtatc ccccgccgcg gcgacgctgg agaagttccg
     3181 ggagctggac ctggacctga tccccgccac cggcctgctg cccgcggtgg tgccgggcgc
     3241 attcgacgcc tggtgcctgc tgctgcgcga ctggggcagc tgggagctgc cggacgtgct
     3301 gagcttcgcc atcgactacg cgcgcaacgg catccatatc gtgccgcgca tcagcgcgca
     3361 gatcgaaagc gtgcggccgc tgttcgagca ggaatggaag acatccgccg cggtgttcct
     3421 gcctggcggc aaggtacccg cgccggaatc gctgttcgcc cgtcctgccg tggccgacac
     3481 gtatgagcgc atctgcaagg aagccgtcgg cgacacccgc gaggccc
//
LOCUS       DASSZH010000440         2308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1186653, whole genome shotgun sequence.
ACCESSION   DASSZH010000440 DASSZH010000000
VERSION     DASSZH010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186653"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VFN42_09540"
     CDS             <1..425
                     /locus_tag="VFN42_09540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8996898.1"
                     /translation="DAVAQAAEITLDTELPDGLPPVRGDERRLLQVLNQLLSNAVKFT
                     EAGGTVTVGARREDDCTLLIVVRDTGMGIAPEQLERVFEPFSPIDAAVSPRCQGASLY
                     LSRALVARHGGRLTLRSRVGEGTTAEIRLPTTTVGTPA"
     gene            559..1401
                     /gene="fabI"
                     /locus_tag="VFN42_09545"
     CDS             559..1401
                     /gene="fabI"
                     /locus_tag="VFN42_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015074825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HET8996899.1"
                     /translation="MPESAPAGLPVKGTIMQGRRGLIMGVANERSLAWGIAAACAAQG
                     AELAFTFQGEELERRVRPLAASVGSSLVFPCDVADEASIDAAFDGLREHWDGLDFLVH
                     AIGYADKQYLRGRFLDTPRGAFLQALDISCYSFVAVGQRAVPMMRAGGSLLTLSYMGA
                     ERWTPHYNVMGVAKAALEASVRYMAADLGGQGIRVNAISAGPIKTLAASGIGDFRYIL
                     RWNQLNSPMQRNVTIEEVGGAGVYLLSDLGSGVTGEVHHVDCGYHIVGMKNPAAPDIA
                     IIEG"
     gene            1402..1953
                     /locus_tag="VFN42_09550"
     CDS             1402..1953
                     /locus_tag="VFN42_09550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET8996900.1"
                     /translation="MPAITAESLLALMQEKGSRRYGLHDVSQLQHALQAATLAEQEGC
                     APTLIVAALLHDIGHMVHGLGDNPAAEGLDDRHEAIGHAYLATCFGPEVTEPVRLHVA
                     AKRYLCATEADYFSKLSPDSVLSLSLQGGPMSDAEVAAFRALPQARNAVRLRRFDEAA
                     KVKDLPTPPLAHFHPHLDSCLAP"
BASE COUNT          359 a          773 c          816 g          360 t
ORIGIN      
        1 cggatgccgt tgcacaggcg gcggagatca cgctggatac cgagctaccg gacggcctgc
       61 cgccggtacg aggcgacgag cggcgcctgt tgcaggtgct gaaccagttg ctgtccaacg
      121 ccgttaagtt caccgaagcc ggcggcacag tcaccgtcgg ggcgcgtcgg gaagatgatt
      181 gcacgttgct gatcgtggtg cgcgataccg ggatgggcat cgccccggaa cagctggagc
      241 gggtattcga gcccttcagc ccgattgacg ccgcggtatc tccccgctgc caaggcgcca
      301 gcttgtacct atcgcgcgcg ctggtagccc gccatggcgg gcggctgacc ttgcggagcc
      361 gcgtcggcga aggtaccacc gccgaaatcc gcctgcccac gacgacagtg ggaacgcccg
      421 cctaagcggc ttcatcggta cggattcgcg ctgggaattt gcccggccca aaacctgccg
      481 gccctaccct tgccgtgcgc cgcatccctg tgccaccaca ggctgcaaca ctccgcaact
      541 gccccccaga ggacctgcat gcccgagagc gctcccgccg gcctgcccgt gaagggcacg
      601 atcatgcagg gtcggcgcgg cctgatcatg ggggtggcga acgaacgctc gctggcctgg
      661 ggcatcgcgg ccgcctgtgc cgctcagggc gcggaactcg ccttcacctt ccagggcgag
      721 gagctggagc ggcgcgtgcg cccgctggcc gcttcggtcg gatcctcgct ggtattcccc
      781 tgcgacgtgg cggatgaagc cagcatcgac gccgctttcg acgggctgcg cgagcactgg
      841 gacgggttgg atttcctggt gcatgcgatc ggttatgcgg acaagcagta tctgcgcggg
      901 cgcttcctgg acacgccgcg cggcgcgttc ctgcaggcgc tggatatctc ctgctactcc
      961 ttcgtcgcgg tgggccagcg cgcggtgccg atgatgcgcg cgggcgggtc gctgctgacg
     1021 ctaagctata tgggcgcgga gcgctggaca ccgcactaca acgtgatggg cgtcgccaag
     1081 gccgcgctgg aggcctcggt acgctacatg gcggccgatc tgggcggcca gggcattcgg
     1141 gtgaacgcga tcagcgccgg gccgatcaag accctggcgg ccagcggcat cggtgacttc
     1201 cgctacatcc tgcgctggaa ccagctcaat tcgccgatgc aacgtaacgt aaccatcgag
     1261 gaagtcggcg gcgcgggggt gtatctgttg tccgacctcg ggtcgggcgt caccggcgag
     1321 gtgcatcatg tcgattgcgg ctaccacatc gtcggcatga agaacccggc ggcgccggac
     1381 attgccatca tcgagggatg aatgcccgcg atcaccgccg aatccctgct ggccctgatg
     1441 caggagaagg gcagccgccg ctacgggctg catgacgtca gccagttgca gcacgcgctg
     1501 caggcggcga ccctggcgga gcaggaaggc tgcgcgccga ccctgatcgt ggcggcgctg
     1561 ctgcacgata tcggccacat ggtgcacggg ctgggcgaca acccggccgc cgagggcctg
     1621 gacgacaggc acgaagcgat cgggcacgct tatctggcga cctgcttcgg cccggaagtg
     1681 accgagccgg tgcgcctgca cgtcgcggcc aagcgctacc tgtgcgcgac cgaggcggat
     1741 tatttcagca agctgtcgcc ggacagcgtg ctgagcctgt cgctgcaggg cggccctatg
     1801 tcggacgcgg aagtcgcggc attccgcgcg ctgccgcagg ccaggaacgc ggtgcggctg
     1861 cgccgcttcg atgaggcggc gaaagtgaag gacctgccga cgccgccact ggcgcatttc
     1921 catccgcacc tggattcctg cctggcgccg tagcaagccc gggcggcctt catgcgcgcg
     1981 tgctgctggc gcgctggaac gacgcatccc gcctgcgccg cgttctgccg acagacgcaa
     2041 gggagatccg catggacagc tcgccgatcg tcatcgtgct tgtgccggcc atcgtcgcgg
     2101 aggtgctgaa cgcagccgtg ggtcaggatc ggggtcaggc acgggcctga cagccgcgcc
     2161 ggcttcaccc cttggcgcgg cacgaatcgg aatggcggga ccgccgtgcg gtggccccgt
     2221 tccctgcctg agcagactcg cgcgccaggc ctggcgcgcg cggattcagg gcggcccggc
     2281 gcaggcggcg taggacagcc gccgcggc
//
LOCUS       DASSZH010000441         3544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_794610, whole genome shotgun sequence.
ACCESSION   DASSZH010000441 DASSZH010000000
VERSION     DASSZH010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794610"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /locus_tag="VFN42_09555"
     CDS             complement(<1..1304)
                     /locus_tag="VFN42_09555"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8996901.1"
                     /translation="MCLARGIASGKWPCRARRRGLSIFCSDRNDIREIQGPRASQGDA
                     ATLLRSGRTRQENGMSPANWPLVVIARSHDSRLLILVRLIRSIGQGALVVDFALYLRA
                     LHWSGGAIGALLTASMVSGIALTAVLGPASDRIGRKPLLLALEATRIAAAIVALLTSA
                     PVPLAVAAIAAQYGRGGNGTAGPFGAVEQAWLAQSATPDTRARIFSLNSSMGFFGRTL
                     GALLAALPGLLVGWLPGAMSFRPLFGVVALSSLIAFCLILRIPEDTATQRTETKRPAR
                     QDAAPRIQTAHSENTLLLRLALANLLQGAGIGLTGPLIAYWFALRFHAGPGVIGPVMA
                     AGFLMAAAASIVNGRLAQRWGVVPVVVGMRATGIGLLVALPLLPTLPVAAVAYMVRSV
                     FNRGTNGVRAALSMSLVRPERRGFAATVSSVSVSVPRAIGPVV"
     gene            complement(1207..1998)
                     /locus_tag="VFN42_09560"
     CDS             complement(1207..1998)
                     /locus_tag="VFN42_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996902.1"
                     /translation="MTADRIARLVAEPPAECHLFLPDALDPTTPLLVCVHGISRNAEE
                     HLSNFAPLAAAHGVAVLTPVFAPAPFPDYQRLGRQGRGLRADLVLRAMLDRLGEQCGL
                     APERIHLFGHSGGAQFVHRYAMAYPTQVARYVVSAAGWYTLPDCSLPYPFGTALDWNL
                     PGIAFDPSAYLRVPGAVLVGTKDRRRGTTLRQAPFLDAAQGRTRIERAKHWVAAMNAA
                     ATARGLLPPLSFELVPGAGHRFGEMAVQGAAARAFNFLFGSERHT"
     gene            complement(2246..3334)
                     /locus_tag="VFN42_09565"
     CDS             complement(2246..3334)
                     /locus_tag="VFN42_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996903.1"
                     /translation="MSASATLWHPSMTAPESPPAALAPHRAAAAKRAYTTRRVPDERM
                     QHLLQDVAPQPGDLVLARVARVGQHQHIEQVSGRRAKLWQDDEIIVAYGNRYAPDQFE
                     AFVPEDLGPCDLVAGGGVAARVCSQHPAMKAATRIEPIGLLADHAGRRINLSDHALRL
                     AAIEGPLPPVLAVVGCAMNAGKTTTAAGAIRGLSRNGMRVGAAKVTGTGSGGDRWAMV
                     DAGAALVLDFTDAGHASTFGLTGTRLTQLLPLLISQLAAHRTDAIVLELSDGLFQQET
                     ATMIESEAFARLVNGVLFAAGGAMGAKAGTDWLRERGRPAMAVSGVVSASPLARRETE
                     AATGLPVLTLADLAAGEWCHAADSWFGV"
BASE COUNT          589 a         1239 c         1218 g          498 t
ORIGIN      
        1 accaccggcc caatggcgcg cggcaccgag accgatacgg aactgaccgt tgccgcgaag
       61 ccccgccgct ccggccggac gagactcatg ctcagcgcgg cgcgcacgcc gttggtgccg
      121 cggttgaaca ccgagcggac catgtaggcc accgcggcaa ccggcagcgt cggcagcaac
      181 ggcaatgcca ccagcagacc gatgccggtg gcacgcatac cgaccacgac gggcacgacg
      241 ccccagcgtt gtgccaggcg gccgttcacg atcgacgctg ccgccgccat cagaaagccg
      301 gccgccatca caggcccgat cactcccgga ccggcgtgga agcgcagtgc gaaccagtag
      361 gcgatcagcg ggccggtaag cccgatgcca gcgccctgca gcaggttggc aagcgccagg
      421 cgcaacagca gtgtattctc gctatgggct gtctggatgc gtggtgctgc atcctgccgc
      481 gcgggacgct tcgtctctgt ccgctgcgtt gcggtgtctt ctggaatccg caggatcagg
      541 cagaacgcga tcaatgagct gagtgccacg acgccgaaca gcggtcggaa actcatcgcg
      601 ccgggcagcc agccaacaag caggcccggt agcgccgcga gtaaagctcc caatgtacgg
      661 ccaaagaatc ccatactgga attcaggctg aagatgcggg cccgcgtgtc cggggtggct
      721 gactgggcca accatgcctg ttccacggcg ccgaacggac cggcggtgcc attgccgcca
      781 cggccatact gcgcggcgat tgccgccacc gccagcggca ccggcgcgct ggtcagcagt
      841 gccacaatgg ccgcggcgat gcgggtcgct tccaaggcga gcagcaaagg cttgcggcca
      901 atacggtcgc ttgccgggcc gaggaccgcg gtcagggcga tgccggaaac catcgaggcg
      961 gtgagcaggg ccccgatcgc gccgcccgac cagtgcaggg cgcgcaggta aagcgcgaag
     1021 tccaccacca acgcaccctg gccgatgctg cggatcagcc gcaccaggat cagcaggcgg
     1081 ctgtcgtggc tacgggcgat gacgaccaga ggccaatttg ccgggctcat accattctcc
     1141 tgacgcgttc gcccgctgcg aagaagcgtt gctgcgtcac cctgcgatgc gcgtggacct
     1201 tggatttcac gtatgtcgtt ccgatccgaa cagaaaattg aaagcccgcg ccgccgcgcc
     1261 ctgcacggcc atttccccga agcgatgccc cgcgccaggc acaagttcga agctcaacgg
     1321 cggcaacagg ccgcgggctg tcgcggcagc gttcatcgcc gcgacccagt gctttgcccg
     1381 ttcaatgcgg gtgcggcctt gcgccgcatc cagaaagggc gcctggcgca gggtggtgcc
     1441 acgcctgcga tccttggttc cgaccagtac cgccccgggg actcgcagat acgccgaggg
     1501 gtcgaacgcg attcccggca ggttccagtc cagggcggtg ccgaacgggt agggcaggga
     1561 gcaatccggc agcgtgtacc agccggcggc gctgaccaca taacgggcta cctgggtggg
     1621 ataggccatc gcatagcggt gcacgaactg tgctccgccc gaatgtccga acaggtgaat
     1681 ccgctccggc gccaggccgc actgctcgcc aagccggtcc agcatggccc gcaacacgag
     1741 atcggcgcgc aggccccggc cctgccggcc aagccgctga tagtcgggaa atggcgccgg
     1801 tgcgaacacc ggggtcagaa ccgccacgcc atgtgccgcg gccagcggcg cgaagttgct
     1861 cagatgctct tcggcgttgc gcgaaatgcc atgcacgcag accagcagtg gcgtggttgg
     1921 atcgagtgca tccggcagga acaggtgaca ctcggccggt ggctccgcga caagccgggc
     1981 gatgcgatcg gccgtcacta gtaccggctg acgtgcaaca tgacccggcc ctcgcggtcc
     2041 gaggcgtggc gggcgcagcg gtctgtcatc ccattgccgg aagcgcccag gggtgcgcac
     2101 cacgccatgg cggaatgccg gccatccaca acagttcctg gtcgcgccgg gccaagttgt
     2161 tggtggcgtg cctgcgtccg ccacgacgcg gtcacggccg gcctgggaag cgctgccgag
     2221 cctcccgtgg cctgtgccgg cgccatcata cgccgaacca ggaatccgcc gcgtggcacc
     2281 attcaccggc cgccagatcc gccagcgtca acaccggcag cccggtcgcg gcttccgttt
     2341 cacgcctggc caggggcgat gccgacacga cgccgctgac cgccatggcc ggccggccac
     2401 gctcgcgcag ccaatcggtg cccgccttgg cgcccatcgc gccgccggcg gcgaacagca
     2461 caccgttcac cagccgggca aaggcttccg attcgatcat tgtcgccgtt tcctgctgga
     2521 acaggccgtc gctgagttcc agcacgatcg cgtccgtccg gtgcgcggcc agttggctga
     2581 tcagcagggg cagcaactgc gtcagccgcg tgccggtcag gccgaaggtg gaagcatggc
     2641 cggcatcggt gaagtccagc accagcgccg caccggcatc gaccatggcc cagcggtcgc
     2701 cgccggagcc ggtgccggtg accttggccg cgccgacccg catgccattg cgggacaggc
     2761 cgcggatcgc accggccgcc gtggtggtct tgccggcatt catggcgcag ccgaccacgg
     2821 ccagcaccgg cggcagcggg ccttctatcg ctgccaggcg tagcgcatgg tcggacaggt
     2881 tgatgcggcg tccggcatgg tcggcgagca ggccgatcgg ctcgatgcgg gtcgctgcct
     2941 tcatggcggg atgttgactg catacccggg cggccacgcc gccgccggcg accagatcgc
     3001 aggggccgag gtcctcgggg acgaacgctt cgaactggtc cggcgcatag cgattgccat
     3061 aagcgacgat gatctcgtcg tcctgccaca gcttggcgcg tcgtccgctc acctgctcga
     3121 tgtgctgatg ctggccgacc cgcgcgacgc gcgccagcac caggtcgccc ggctgcggcg
     3181 cgacgtcctg cagcaggtgc tgcatccgct catccggcac gcgccgggtc gtgtaggcgc
     3241 gtttcgccgc cgcggcgcgg tgtggggcga gagcggctgg cggcgattcg ggcgccgtca
     3301 tggaagggtg ccaaagcgtg gcgctggcgg acatcagggt gctccatcgt gacctgcgcc
     3361 ggggggctta gcggacgaag ctgacgggaa cctgatggcg ctcacccggc gccggcgcgt
     3421 ccgagcgaca ggacgaatcg tgtgccgcct tgcggggcgt cctccagccg cacgtcgccc
     3481 gcgtgcagtt gcgcgacgcg gcggaccagc gccagcccga cgcccgcgcc accgccgtgc
     3541 tccc
//
LOCUS       DASSZH010000442         8376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_487625, whole genome shotgun sequence.
ACCESSION   DASSZH010000442 DASSZH010000000
VERSION     DASSZH010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8376
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487625"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VFN42_09570"
     CDS             complement(<1..255)
                     /locus_tag="VFN42_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996904.1"
                     /translation="MSTLLLAEPRAVVAPDPMLPRPVRVQKRTRDLPGTVTLELVSTD
                     GLPLPRYAPGQFTMLYVFGVGEIPVSISGDASDPSCLVQTV"
     gene            complement(233..694)
                     /locus_tag="VFN42_09575"
     CDS             complement(233..694)
                     /locus_tag="VFN42_09575"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET8996905.1"
                     /translation="MQGLERILGEHRLFAGLGQPFLELAAGCAKNVRFDPDDFLFHAD
                     DPADWIYLVRHGRVALEIATPHGGTIRFETVSEGEVVGLTWLLPPYRWGYDARATELT
                     RAIALDARCLRDKCEADHDLGYEVLKRFLPVLVQRLQATRLQMLDVYAPAR"
     gene            complement(681..1862)
                     /locus_tag="VFN42_09580"
     CDS             complement(681..1862)
                     /locus_tag="VFN42_09580"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET8996906.1"
                     /translation="MNDETSRTGVVRDQPGSPLHAAVITVDGLAAMIAALRAQGFRLL
                     GPTRRDDAIVYDDIGSLGDLPAGWTDEQDGGHYRLRRRGDDALFGYAVGPHAWKRFLH
                     PPLLTLWRAERDADGMRVTADPPPPQRLALLAVRSCELHAIAIQDRVFLGGPHVDPHY
                     QARRDGAFIVAVNCGVAGGTCFCVSMQTGPRAEAGFDLALTELLDGEHRFLVEVGSER
                     GAALLAELPHRPAAPDDLARSAAVLAHAAAGMGRQMQADKVHDLLLNNLQHKRWDDVA
                     ARCLSCGNCTQVCPTCFCTSVSDHNDLATGAAERTRRWDSCFTMDFSYIHGGSVRASP
                     KSRYRQWMTHKLANWFDQFGTSGCVGCGRCITWCPVGIDITEEVRAIRGGAAEDPHAG
                     T"
     gene            2063..3565
                     /locus_tag="VFN42_09585"
     CDS             2063..3565
                     /locus_tag="VFN42_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HET8996907.1"
                     /translation="MSDVPATRVGGQHHTMPSGRFWRVAIPVAITLMLAALPPPHGLA
                     QHAWYYFAVFAGVIAALITEPLPNPAVGLVGLTLMAGLSRWVLFSPADQAKAGFKITG
                     QTIQWALSGFSSTTVWLVGAAFMFALGYQKTGLGRRIALLLVRALGRNTLSLGYATTF
                     AETLLAPFTPSNTARGAGIMFPIVSNLPGLFDSRPNDPSARRIGGYIMWTTFAADCIT
                     STLFLTACAPNFLAISLVDKIAHVHITFGDWIRASLPFALPLLLISPLLAYVLYPPEI
                     KRSNEASRWASRELEEMGRVSRREVILSLIVFGAILMWVFGGKLLDPALTAFCGIALM
                     LALGVVTWDDMAKNNAAWTTIMLLATLVTLAEGLAKAGFIAWFAAFVAAHVGGMSPTV
                     MIMALVAIYFLSHYMFASLTAHTTAMLPLMLAAAISIPGMPVATMALALALTTGLMGV
                     ISPYATGPALPYYNSGYIPPAQFWRLGAIFGLIFVAALLLVGVPLLMLGW"
     gene            3649..4620
                     /locus_tag="VFN42_09590"
     CDS             3649..4620
                     /locus_tag="VFN42_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8996908.1"
                     /translation="MPDQTNPLDPALFRPEAVAPETKALNDTMVKLLTPQPDWWVVGA
                     ETTRAARRRGDGPFPAPVFSSRARTISIPGKDGNQIPLRVIAPAGTPRGVYLHIHGGG
                     WVLGAADLQDPMLERVADSTGLAVVSVEYRLAPEHPYPAGPDDCEAAALWLVRNARGA
                     FGTDLLTIGGESAGGHLSAATVLRMRDRHDYTGFRGANLVYGAYDLSLTPSQRRFGDT
                     RLVLRTIDIVQFGNAFLPGITERRVPDISPLYDDLHGLCPALFSVGTADALVDDTLFM
                     YGRWIAAGNAAELAIYPGGAHGFTLFPNTLAQQAEARMHAFLRQAVE"
     gene            4752..5570
                     /locus_tag="VFN42_09595"
     CDS             4752..5570
                     /locus_tag="VFN42_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HET8996909.1"
                     /translation="MSDPREALEAIGLLPDIEIDIADAALQLARVDAPHADWQAARAH
                     LSELARHAVALPATDNLADRSEALSALLATRHGYIGDTQNYDDLANANLIRVIERRRG
                     LPVALGVLWIHAARAAGWGAHGVDFPAHFLVALEGKSTQAVLDVFHGGAALGARELRV
                     LLKQAEGEEAELRPGLLRPMNARRVLLRLQNNIVTRRLQAGDLPGALTCTEDMLRIAP
                     DSAELWRQAAVMNQRLERVGAALRCFERCLELVPAGGAAARVRMAMDELRSRMN"
     gene            complement(5585..5896)
                     /locus_tag="VFN42_09600"
     CDS             complement(5585..5896)
                     /locus_tag="VFN42_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996910.1"
                     /translation="MYLARFSYDVLPVNRDAAIGFIRREAEAARKNGLSARILVPLTR
                     GPNGAALQFEVELNNLDQLDKFRQRGVGSAQETGNWMRDFSEVLERPPEVEVLRLDGG
                     G"
     gene            6298..7473
                     /locus_tag="VFN42_09605"
     CDS             6298..7473
                     /locus_tag="VFN42_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HET8996911.1"
                     /translation="MRPLEGIRVIALEHAVAAPICSRHLADLGADVIKIERPGDGDFA
                     RAYDSVVHGASSFFIWLNRGKRSITLDLKHDEGRAVLQRLVDGADVLLQNLAPGAAAR
                     LGLSHAALQPRNPRLVVCDISGYGEGGPFAHKKAYDLLIQAESGLISVTGSEDAPSRV
                     GISAADIATGMYAFSGILAALLRRERNGEGANVKIAMLDALGEWMMYPMYRAAFTGAP
                     PPRVPTSHPLLAPYGAHRTQDGQVIFGLQNEREWATFCTRVLRQPEMQTDLRFNSMAA
                     RRDNRQALTAMIEDLFTTMTSVEVVRLLDEAGIANGRLNESIDVWNHEQFAARDKWRE
                     VNTEVGPVRALLPPFTFTDFEAAMGEVPALGQHTDAVLREAGYNDAEIAGLHRAGAL"
     gene            7500..7964
                     /locus_tag="VFN42_09610"
     CDS             7500..7964
                     /locus_tag="VFN42_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET8996912.1"
                     /translation="MVPEQRWFEDFRIGERFVLPSRTMTEAVFLAFQAASGDNHPVHY
                     DAEYCKARGMPAMLAHGFQTLVQTAAGAGLFPFVTEESLAGFIEQSSRFLKPVFAGDT
                     LYPALEVEELSPNRSTGVVGLRSTVHNQRRELVLDGRQRYLLRRRPGQDAPG"
     gene            complement(7980..8138)
                     /locus_tag="VFN42_09615"
     CDS             complement(7980..8138)
                     /locus_tag="VFN42_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996913.1"
                     /translation="MAEHQTTVFRGPETPEAFLADRQRFWEGWTRFTLTSLIFIVLLL
                     IAMAVFLL"
     gene            complement(8180..>8376)
                     /locus_tag="VFN42_09620"
     CDS             complement(8180..>8376)
                     /locus_tag="VFN42_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HET8996914.1"
                     /translation="PILAQAAVPVLPGDTEDTLADRVLAQEHVIYPAALHAFAAGTAG
                     MPADAAATLRNPLPAAASVP"
BASE COUNT         1313 a         2858 c         2878 g         1327 t
ORIGIN      
        1 gacggtctgc accaggcagg acggatccga cgcgtcgccg ctgatgctga cggggatctc
       61 ccccacgccg aacacgtaca gcatggtgaa ctgccccggc gcatagcgcg gcagcggcag
      121 cccatcggtg gagaccagct ccagcgtcac cgtccccggc aggtcgcgcg tgcgcttctg
      181 cacgcgtacc gggcgcggca gcatcggatc cggcgccacc acggcgcgcg gttcagcgag
      241 caggagcgta gacatcgagc atctgcaacc gggtggcctg caggcgctgc accagcaccg
      301 gcaggaaccg cttcaggact tcgtacccca gatcgtggtc ggcctcacac ttgtcgcgca
      361 ggcagcgcgc atcgagcgct atggcgcggg tcagctcggt cgcccgcgcg tcatagcccc
      421 agcgataggg cggcagcagc caggtcagtc cgacgacctc gccttcgctc accgtctcga
      481 agcgtatcgt gccgccgtgc ggcgtggcga tctccagagc cacgcggcca tgccgcacca
      541 gatagatcca gtcggccgga tcgtccgcgt ggaacaggaa gtcgtccgga tcgaagcgca
      601 cgttcttggc gcagccggcg gcgagctcca gaaatggctg gcccagcccg gcgaacagcc
      661 tgtgctcgcc gaggattcgc tcaagtccct gcatgcgggt cctccgctgc gccgccacgg
      721 atggcgcgca cttcctcagt gatgtcgatg ccgaccggac accaggtgat gcagcgtccg
      781 cagccgacgc agcccgaagt gccgaactgg tcgaaccaat tggccagctt gtgggtcatc
      841 cactggcggt agcgcgattt tggtgaggcc cgcacgctac cgccatggat gtaggagaag
      901 tccatggtga agcaggaatc ccagcgccgc gtgcgctccg ccgcgccggt ggcgaggtcg
      961 ttgtggtccg acacgctggt gcagaagcag gtcgggcaga cctgcgtgca gttgccgcag
     1021 gacaggcagc gtgcggcgac atcgtcccag cgcttgtgct gcaggttgtt cagcagcagg
     1081 tcgtgcacct tgtccgcctg catctgccgg cccatgccag ccgcggcatg cgcgagcacg
     1141 gcggcggagc gtgcaagatc gtccggtgcc gccggccgat gcggcagttc cgcgagcagt
     1201 gcggcgccgc gttcgctgcc gacttccacc aggaagcggt gctcgccatc cagcagttcc
     1261 gtcagggcca ggtcgaagcc ggcctccgca cgcggcccag tctgcatcga cacacaaaaa
     1321 cacgtgcctc ccgccacacc gcaattcacc gcgacgatga aggcgccgtc ccgccgagcc
     1381 tggtagtgcg gatcgacgtg cgggccgcca aggaacacgc ggtcctgaat ggcgatcgcg
     1441 tgcagctcgc acgatcgcac ggcaagcagg gccaggcgtt gcggcggcgg cggatcggcg
     1501 gtgacgcgca tgccgtcggc atcgcgttcg gcgcgccaca aggtcagcag cggcgggtgc
     1561 aggaagcgct tccaggcatg tgggccgacc gcgtagccga acagggcatc gtcgccgcgc
     1621 cggcgcagcc ggtagtgccc gccatcctgt tcatcggtcc agccggcggg cagatcgcca
     1681 agggatccga tatcgtcata aacgatcgca tcgtcccgcc gcgtgggacc gagcaggcga
     1741 aagccctggg cgcgtaacgc tgcgatcatg gcggcgaggc catccaccgt gatcaccgcg
     1801 gcatgcagcg gcgagcccgg ctggtctcgc acgacaccgg tgcgggaggt ctcgtcattc
     1861 atgctgcaaa cctgcctcca aatccgcgtc tcgcgccttg atctgcctca atgcgattgg
     1921 ccgcgcgatg tgcgagccaa cactcggcat tctgccgcgc cgagatgagg gaattgttaa
     1981 cggcttcacg tgcatgatac cgctctgcgc gtgagacttc cggctgccgc gcacagcttc
     2041 ggcccttgca ggaggtcgcg acatgtcgga cgtccccgcc acccgcgtcg gcgggcagca
     2101 tcacaccatg ccgtccggcc gcttctggcg cgtggcaatc ccggttgcca tcacgctgat
     2161 gctggctgcc ttgccgccac cgcacggcct ggcgcagcac gcgtggtact atttcgcggt
     2221 cttcgccggg gtgatcgcgg cgctgatcac cgagccgctg ccgaacccgg cagtcggcct
     2281 ggttggcctg acactgatgg cgggactgtc gcgctgggtg ctgttcagcc cggccgatca
     2341 ggcgaaggcc gggttcaaga tcaccggcca gacgatccaa tgggccttgt cgggtttctc
     2401 cagcacgacg gtatggctgg tcggcgccgc tttcatgttc gcgctgggct atcagaaaac
     2461 cggtctggga cgccgcatcg ccctgctgct ggtgcgcgcg ctgggccgca acacgctgtc
     2521 cctcggctac gccaccacat tcgccgaaac gctgctggca ccgttcacgc cgtccaacac
     2581 cgcgcgcggc gccggcatca tgttcccgat cgtcagcaac ctgccgggcc tgttcgactc
     2641 cagaccgaac gacccgtcgg cgcgcaggat cggcggctat atcatgtgga caacgtttgc
     2701 ggcggattgc atcaccagca cgctgttcct caccgcctgc gcgccgaact tcctggcgat
     2761 cagcctggtc gacaaaatcg cgcatgtgca catcaccttc ggcgactgga tcagggcgtc
     2821 gctgcccttc gccctgccgc tgctgctgat atcgccgctg ctggcgtacg tcctgtatcc
     2881 gccggagatc aagcgcagca atgaggcctc gcgctgggcc tcacgggaac ttgaggaaat
     2941 ggggagggtg tcgcggcgcg aggtgatcct gtcactgatc gtgttcggcg ccatcctgat
     3001 gtgggtgttc ggcggcaagc tgctcgatcc ggcgctcacc gcgttctgcg gcatcgcgct
     3061 gatgctggcg ctgggcgtag tcacctggga cgacatggcg aagaacaacg cggcctggac
     3121 cacgatcatg ctgctggcca cgctggtgac gctggcggaa ggtctggcca aggcggggtt
     3181 catcgcgtgg ttcgccgcct tcgtggcggc gcatgtcggt ggcatgtcgc ccacggtgat
     3241 gatcatggcg ctggtcgcga tctacttcct ctcgcactac atgttcgcca gcctgaccgc
     3301 gcataccacg gcgatgctgc cgctgatgct ggcggcggcg atcagcatcc ccggcatgcc
     3361 ggtggcgacc atggcactgg cgctggcgct gaccaccggg ctgatgggcg tgatctcgcc
     3421 gtacgcgacc ggcccagcgc tgccgtacta caacagcggc tacatcccgc cggcgcagtt
     3481 ctggcggctg ggcgcgatct ttggcctgat cttcgtcgct gcgctgctgc tggttggcgt
     3541 gccgctgctg atgctcggct ggtaaccgga accgccgcgg gcgcggcgcg ggttgctgcc
     3601 ctggcctgag gccggacaag atcgggcctc agccacggag gagccggcat gccggaccag
     3661 accaacccgc tcgatcccgc cctgttccgc cccgaggcgg tggcaccgga aaccaaggcg
     3721 ctcaacgaca cgatggtgaa gctgctgacc ccgcagccgg attggtgggt ggtcggggcc
     3781 gaaaccaccc gcgccgcccg gcggcgcggc gacgggccgt tcccggcccc ggtgttttcc
     3841 tcccgggccc gcaccatcag catccccggc aaggacggca accagatccc gctgcgggtg
     3901 atcgcgccgg ccggcacacc gcgcggcgtc tatctgcata tccatggcgg cggctgggtg
     3961 ctgggggcgg ccgacctgca ggatccgatg ctggagcgcg tggccgacag taccgggctg
     4021 gccgtggtca gcgtggaata ccggctggcc ccggaacacc cctacccggc cgggccggat
     4081 gactgcgaag cggcagcgct gtggctggtg cgcaatgcgc gcggcgcgtt cggcaccgac
     4141 ctgctgacca tcggcggcga gtcggcgggc ggccatttgt ccgcggccac cgtgctgcgg
     4201 atgcgcgatc ggcacgacta taccggcttc cgcggcgcca acctggtgta tggcgcctac
     4261 gacctgtcgc tgacccccag ccagcggcgt ttcggcgaca ccaggctggt gctgcggacg
     4321 atcgacatcg tgcagttcgg caacgccttc ctgcccggga tcacggaacg gcgggttccc
     4381 gacatctcgc cgctgtacga cgatctgcac gggctttgcc cggcgctgtt ctcggtcggc
     4441 acggcggacg cgctggtgga cgacacgctg ttcatgtacg ggcgctggat cgcggcgggg
     4501 aacgccgcgg aactggcgat ctaccccggc ggggcgcacg gcttcaccct gtttccgaac
     4561 acgctggcgc aacaggcgga ggcgcggatg cacgcattcc tgcggcaggc ggtggaatag
     4621 taccgggccg ccccgggagt cgaacgcgtc ccgcacggga actgcggctt ttctgcgccc
     4681 tggcccgact tgcttgcgga ccgggcccgc cgcgcccatc gtgaagctgc gacaaccgga
     4741 gccctggatg gatgtctgac ccgcgtgaag cgctcgaagc gatcggcctg ctgccggata
     4801 tcgagatcga tatcgccgat gccgcactgc aactcgcccg cgtcgatgcg ccgcacgcgg
     4861 actggcaggc ggcgcgcgcg catctgtccg aactcgcccg ccatgccgtc gcgctgccgg
     4921 caaccgacaa tctggccgac cgctcggaag cgctgtcggc gctgctggcc acgcgccacg
     4981 gctatatcgg cgacacgcag aattatgacg atctggccaa tgccaacctg atccgggtaa
     5041 tcgagcgccg ccgcggcctg ccggtggcgc tcggcgtgtt gtggatccac gccgcccgcg
     5101 ccgcaggctg gggcgcgcat ggcgtggatt tcccggcgca tttcctggtg gcgctggagg
     5161 gcaagagcac gcaggcggtg ctggacgtgt tccacggcgg tgccgcgctg ggcgcccgcg
     5221 agctgcgcgt gctgctgaag caggcggagg gcgaggaggc cgaactgcgc cctggcctgt
     5281 tgcggccgat gaatgccagg cgggtgctgc tgcgcctgca gaacaacatc gtcacccggc
     5341 ggctgcaggc cggcgacctc ccgggggcgc tgacctgcac cgaggacatg ctgcgcatcg
     5401 ccccggacag cgcggagctg tggcgccagg cggcggtgat gaaccagcgg ctggagcggg
     5461 tcggcgccgc tctccgctgc ttcgagcgtt gcctggaact ggtgccggcc ggtggtgcgg
     5521 cggcgcgcgt gcgcatggcg atggacgaac tgcgcagccg gatgaactga ccggcgcgtg
     5581 gcgctcagcc gccgccgtcc agccgcagaa cctccacttc cggcgggcgc tccagcacct
     5641 cgctgaaatc gcgcatccag ttgccggttt cctgggcaga cccgacgccg cgctggcgga
     5701 acttgtccaa ctggtcgagg ttgttcagtt ccacttcgaa ctgcaacgcc gcaccgttcg
     5761 gtccgcgggt caggggcacc agaatccgtg cgctcaggcc atttttgcgt gcggcctccg
     5821 cctcgcggcg gatgaaaccg atcgcggcgt cgcggttgac cggcagcacg tcataggaaa
     5881 agcgggcgag atacatggcg gggtctcctg cctgtccaga ttcgtcccgc gggagaaacg
     5941 cgccgccgct gcaccggttt gctcgcgcgc cgccgaattg catgcggtgg tctgatgccg
     6001 ctccggctca tcggtaacgc ccggtcaatc atggcggcgc ataaacacga acgccggcca
     6061 ggatggtcct gaccggcgtc agtgggagaa aacccgatga agcaccatcg aggtcttctg
     6121 ccgcgaatat tggtgatcct ggccatcaag gtcaagatca tcatacgccg ccggtagacg
     6181 gcggagcagc ctgggaaacc aggctgctcc actcccacgc gacgccggcc ggcgctttga
     6241 caccgcccgc gcccggcgct ttgttcgccc gacaacatcc gggcgaggaa acataccgtg
     6301 cgacccctgg aaggcatccg cgtcatcgcg ctggaacacg ccgtcgcggc accgatctgc
     6361 agccggcatc tggccgacct cggcgccgat gtgatcaaga tcgagcggcc cggcgatggc
     6421 gatttcgccc gcgcgtatga cagcgtcgtc cacggcgcct ccagcttttt catctggctg
     6481 aaccgcggca aacgcagcat caccctggac ctgaagcacg atgaaggccg cgcggtgctg
     6541 cagcggctgg tggacggcgc cgacgtgctg ctgcagaacc tggcaccggg cgccgccgcg
     6601 cgtctgggcc tcagccacgc agcgttgcag ccgcgcaatc ccaggctggt ggtgtgcgac
     6661 atctccggct acggcgaagg cggccccttc gcgcacaaga aggcctatga cctgctgatc
     6721 caggcggagt ccgggctgat cagcgtcacc ggctcggagg acgcgccgtc gcgcgtcggc
     6781 atctccgccg ccgatatcgc caccgggatg tatgcgttca gcggcattct cgccgcgctg
     6841 ctgcggcggg agcgcaacgg cgaaggcgcc aacgtgaaga tcgccatgct ggacgcgctc
     6901 ggcgagtgga tgatgtaccc gatgtaccgc gccgccttca ccggcgcccc gccgccgcgc
     6961 gtccccacca gccatccact gctcgcgccg tacggcgcgc atcgcaccca ggacggccag
     7021 gtgatcttcg gcctgcagaa cgagcgcgag tgggccacgt tctgcaccag ggtgctgcgc
     7081 cagccagaga tgcagacgga tctgcgcttc aactccatgg cggcgcggcg cgataaccgc
     7141 caagcgctca ccgcgatgat cgaggacctg ttcaccacca tgacctcggt cgaggtggtg
     7201 cggttgctgg acgaagccgg catcgccaac ggccggctga acgagtcgat cgatgtatgg
     7261 aaccatgagc agttcgccgc gcgcgacaaa tggcgcgagg tgaacaccga ggtcggcccg
     7321 gtgcgcgcgc tgctgccgcc cttcaccttc accgatttcg aagcggcgat gggcgaggtg
     7381 cccgcgctgg gccagcacac cgatgcggtg ctgcgcgagg ccggctacaa cgacgcggag
     7441 attgccggcc tgcaccgggc cggcgcgcta tgagcgggtt cgaccctgca gcgcaccgca
     7501 tggtgcccga gcaacgctgg ttcgaggatt ttcgcatcgg cgaacgtttc gttctgccga
     7561 gccggacgat gacggaagcg gtgtttttgg cgttccaggc ggcgagtggc gacaaccacc
     7621 cggtgcacta cgatgcggag tactgcaagg cgcggggcat gccggcaatg ctggcgcacg
     7681 gcttccagac tctggtgcag accgcagccg gcgccgggct gttccccttc gtcaccgagg
     7741 aatcgctggc cggattcatc gagcagtcga gccggttcct gaagccggtt ttcgccggcg
     7801 acacgctgta tccggcgctg gaggtggagg aactctcgcc gaaccgcagc accggcgtgg
     7861 tgggcctgcg cagcacggtg cacaaccagc ggcgcgaact ggtgctggac ggacgccagc
     7921 gctacctgct gcgccggcgg cctgggcagg atgcgcccgg ctgaccggac gacgggcggc
     7981 tacagcagga acaccgccat ggcgatcagc agcaggacga tgaagatcag gctggtcagg
     8041 gtgaagcgcg tccagccttc ccagaaccgc tgacggtcgg ccagaaacgc ctccggcgtc
     8101 tccggcccgc ggaagacggt ggtctgatgt tcggccatcg cggacgtccc gttgctttga
     8161 atcgattgtg cccgagtctt tatggcacgg aagccgcggc gggcaagggg ttgcgcaggg
     8221 tggccgcggc gtcggccggc attccggcgg ttcccgcggc gaaggcgtgc agggccgccg
     8281 gatagatcac atgctcctgt gccagcacgc ggtcggccag cgtgtcctcg gtgtcgcccg
     8341 gcagcacagg aaccgctgcc tgggccagga tcgggc
//
LOCUS       DASSZH010000443         2443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_930982, whole genome shotgun sequence.
ACCESSION   DASSZH010000443 DASSZH010000000
VERSION     DASSZH010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930982"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VFN42_09625"
     CDS             <1..534
                     /locus_tag="VFN42_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HET8996915.1"
                     /translation="SLAASGKDMGGRANPGHDTGKLIRALESFAPVAGRGTRRRLAMP
                     GGTALLLDESYNGNGASMRAALAVLALQPARRRIAVLGDMLELGEAGPAEHAALAAPV
                     AGAADLLFACGPLMRHLFDTVPPHIRAAHAADSAALASIVAKQVAPGDAILVKGSLGS
                     RMKTIVNALDALTAETR"
     gene            536..1624
                     /gene="mraY"
                     /locus_tag="VFN42_09630"
     CDS             536..1624
                     /gene="mraY"
                     /locus_tag="VFN42_09630"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET8996916.1"
                     /translation="MLYNLSRPLADHFFLFNLFRYITFRSGAACLTALVVSFWVGPPL
                     IRWLKSVQGHGQPIRLDGPERHLVEKKGTPTMGGLLILFAMTISTLLWVDLRNGYVWA
                     VLTVTLGYGALGFVDDYLKVTKRNTRGVPGRMKLLVQAAIGLGATYWIVALTRAPLGT
                     DLAIPVFKEVLIPLGIFFPIFGMLVLMGASNAVNLTDGLDGLAIVPAMICAAVFVLIA
                     YLVGNHIFADYLQLNYVPGVGELAVFLASLIGAGLGFLWFNAPPAAVFMGDIGSLSLG
                     GALGAVALATKHEIVLMIVGGLFVVETVSVIIQVFWYKRTGTRVFLMAPLHHHFEKKG
                     WAEPTIVIRFWIIAMILALVGLATLKIR"
     gene            1621..>2443
                     /locus_tag="VFN42_09635"
     CDS             1621..>2443
                     /locus_tag="VFN42_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET8996917.1"
                     /translation="MSSFPSDLFAGQRFAVVGLGKNGLPAAQALAAMGAGVAAWDDSE
                     KTRQAANGVTLRNPAEGPFDFDALVLSPGIPHRLPQPHPVALRALQAGVPILSDAELL
                     FQAVRRAGSRARFAGITGTNGKSTTTALLAHILADAGIPVAAGANLGPAALSLPLLPH
                     DGVYVLEMSSYMLERLGSLRFDAAAMLNLSPDHLDRHGDMAGYAAAKRAIFDRQEPGD
                     LAVVGVDDADSRAMAEALRAGPARLVTVSGPAVLADVEAAEMQTPPPPLAGGGWGE"
BASE COUNT          334 a          847 c          833 g          429 t
ORIGIN      
        1 tctctcgccg cctccggcaa agacatgggt ggccgggcca accccggcca tgacacgggg
       61 aagctcatcc gcgccctgga atcctttgcc ccggtcgccg ggcgcggcac ccgccgccgc
      121 ctcgccatgc ccggcggaac cgccctgctg ctcgacgaaa gctacaacgg caatggcgcc
      181 tcgatgcgcg ccgccctggc ggtcctggca ctgcagccgg cccgccgccg catcgccgtg
      241 ctgggggaca tgctggaact cggcgaggcc ggcccggcgg aacacgctgc cctggcggca
      301 ccggtggcgg gcgcggcgga cctgctgttc gcctgcggcc cgctgatgcg ccatctgttc
      361 gacaccgtgc cgccgcacat ccgcgcggcc catgcggccg attccgcagc gctggccagc
      421 attgtcgcaa agcaggtcgc gcccggcgat gcaatcctgg taaagggcag ccttggcagc
      481 cgcatgaaga ccatcgtcaa cgcgctcgac gcgcttaccg cggagacgcg ctgatatgct
      541 ctacaatctc tcccgcccgc tggcggacca cttcttcctg ttcaacctgt tccgctacat
      601 caccttccgc tccggtgccg cctgcctgac tgcgctggtg gtgagcttct gggtggggcc
      661 gccgctgatc cgctggctga agtcggtgca gggccacggc cagccgatcc gcctggacgg
      721 gccggagcgg cacctggtgg aaaagaaggg cacgcccacc atgggcggcc tgctgatcct
      781 gttcgccatg acgatctcca ccctgctctg ggtggacctg cgcaatggct atgtctgggc
      841 ggtgctgacc gtcacgctgg gctatggcgc gctgggcttc gtggacgact acctgaaggt
      901 caccaagcgc aacacgcgcg gcgtgccggg ccgcatgaaa ctgctggtgc aggcggcgat
      961 cggccttggc gccacgtact ggatcgtcgc gctgacccgc gccccgcttg gcaccgatct
     1021 cgccattccc gtcttcaagg aagtgctgat cccgctgggc atcttcttcc ccatcttcgg
     1081 catgctggtg ctgatgggcg cgtccaatgc ggtgaacctg acggatgggc tggacgggct
     1141 ggcgatcgtg ccggcgatga tctgcgccgc ggtattcgtg ctgatcgcct acctggtcgg
     1201 caaccacatc ttcgccgatt acctgcagct caactacgtc cccggcgtgg gcgaactggc
     1261 ggtgttcctg gcctcgctga tcggcgccgg cctgggcttc ctgtggttca acgcgccgcc
     1321 ggccgcggtg ttcatgggtg atatcggctc gctgtcgctg ggtggtgcgc tgggcgcggt
     1381 ggcgctggcg accaagcatg agatcgtgct gatgatcgtc ggcggcctgt tcgtcgtcga
     1441 aactgtttcg gtcatcatcc aggtgttctg gtacaagcgc accggcaccc gcgtgttcct
     1501 gatggccccg ctacaccatc acttcgagaa gaagggctgg gccgaaccca ccatcgtcat
     1561 ccgcttctgg atcatcgcca tgatcctggc gctggtcggc ctggcgacgc tgaaaatccg
     1621 atgagcagct ttccctccga cctgtttgcc gggcagcgct tcgccgtggt ggggctgggc
     1681 aagaacggcc tgcccgccgc gcaggcgctg gcggcgatgg gcgccggcgt ggccgcctgg
     1741 gacgattccg agaagacgcg ccaggcggcg aacggcgtga cgctgcgcaa ccccgccgag
     1801 ggtccgttcg acttcgacgc cctggtgctg tcgcccggca ttccgcaccg cctgccgcaa
     1861 ccgcatccgg tggcccttcg cgcgctgcaa gcgggcgtgc cgatcctgtc cgacgccgag
     1921 ttgctgttcc aggcggtacg ccgggcgggc tcacgcgcgc ggtttgccgg gatcaccggg
     1981 accaacggga agtccaccac caccgcgctg ctggcgcata tcctggccga cgccggcatt
     2041 ccggtcgcgg ccggggccaa tctcggtccc gctgccctgt cattgccgct gctaccacat
     2101 gatggagtgt acgtcctcga gatgtcctcc tatatgttgg agcgactcgg gtcgctgcgc
     2161 ttcgatgcgg cggcgatgct gaacctctcc cccgatcatc tggaccgcca cggcgacatg
     2221 gccggctacg ctgccgccaa gcgcgcgatc ttcgaccggc aggagccggg ggatctggcg
     2281 gtggtcgggg tggacgacgc ggactcgcgc gccatggccg aggcgttgcg ggcgggaccg
     2341 gcgcggctgg tcaccgtatc cgggccggcg gtgctggcgg acgtagaggc ggcagaaatg
     2401 cagactcctc cccctcccct tgcgggaggg ggatgggggg agg
//
LOCUS       DASSZH010000444         3223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1510786, whole genome shotgun sequence.
ACCESSION   DASSZH010000444 DASSZH010000000
VERSION     DASSZH010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510786"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VFN42_09640"
     CDS             <1..552
                     /locus_tag="VFN42_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996918.1"
                     /translation="VGAGHAIRRTAWQQACGYDEALFFCWEEYDFCLRAIARGWRIQY
                     RGDLVVRHKVSGEQRLAWSGTRWFHFVRNRLYIGRKYGSAWPALLPRFCGYVLKGLRN
                     GAVWQTLRAWPAAMRLAKAGAAETLPPAARVYLRRHDRMPRGGWWARMRQEVLVALPR
                     NFPLQAVEREADPLRAARRHSPR"
     gene            complement(521..823)
                     /locus_tag="VFN42_09645"
     CDS             complement(521..823)
                     /locus_tag="VFN42_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HET8996919.1"
                     /translation="MAKGYWIARVDVTDPTGYQRYVAANAAAFRKFGARFVVRGGRME
                     APEGTPRARNIVLEFSDYDTALACYRSEEYQAAKALRDGCGIADLIVIEGYDGAQP"
     gene            906..1568
                     /locus_tag="VFN42_09650"
     CDS             906..1568
                     /locus_tag="VFN42_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET8996920.1"
                     /translation="MPDGRLIIGNRRYSSWSMRGWLAVRLAQLDVEEVLIPFERPGPT
                     AAIGAVSPNGLVPYLEHKGARVWESLAVCEYCAEQKAGLWPENRVARAMARSVAAEMH
                     AGFRELRQAMWMNLGCDFAGHGRTPGALADIARIEALWAEARRAYGTGGPFLFGAAFT
                     AADVMYAPVVARFLTWQPELSPGARAYCDAVRAHPLVAAWYDAAAREPAEWLLPDYET
                     PR"
     gene            complement(1599..>3223)
                     /locus_tag="VFN42_09655"
     CDS             complement(1599..>3223)
                     /locus_tag="VFN42_09655"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET8996921.1"
                     /translation="SQRILNGNAFGALPCAPESDALCDDEGNKLLDRAGNPITNFLNG
                     GPYSQLNLEAVDSNGYGASLQGLIYYTNLSQRILNGNAFAASPCTPAGNTLCDDEGNP
                     LLDRAGNPITDFLNGGPYSQLNLEAVDSNGYGASLQLTHDSRISGRHNLLVVGISIDR
                     GISTYTASSVLGGITSDNGVFIGPGITIDQRNGPVAPVRLDSTNTYYGAYVADVFDLT
                     PRLALSLAGRMNVAQIDLQDRIGSALTGNHSYTHFNPGIGITYRLVPQVSVYASYSEA
                     NRAPTPAELSCASPQSPCTLANFFTGDPDLKQVVAHTVEAGLRGMLHPATGATLDWKF
                     GLFRTNSDDDILFVASSVPGLAYFQNAGRTRRQGIEAGITWQDSRLHAWANYAFTEAT
                     FQTGQTLFSPLNPAADPAGEIHVVTGDRLPGVPAHRFKFGVSYAVTKAWTVGFSGVVS
                     SGQYLFGDEANLTPKTSPYVVLNINSQYHITPNVEVFGLVQNLLNAQYATYGTYSPTS
                     LVPYAKAPNATDTRSLAPAAPIAAYGGVRIRF"
BASE COUNT          492 a         1007 c         1136 g          588 t
ORIGIN      
        1 gtcggggcag ggcatgcgat ccgccgcacc gcctggcagc aggcgtgcgg ctacgatgag
       61 gcgctgttct tctgctggga ggaatacgat ttctgcctgc gggcgatcgc acgcggttgg
      121 cgcatccagt accgcggcga tctggtggtg cggcacaagg tcagcggcga gcagcgcttg
      181 gcctggtccg gcacacgatg gttccatttc gtccgcaatc ggctgtacat cgggcgcaag
      241 tatggcagtg cgtggcctgc cttgttgccg cgcttctgtg gctatgtgct gaaaggcctg
      301 cgcaacggcg ccgtctggca gaccttgcgc gcctggccgg cggcgatgcg gctggccaag
      361 gcaggcgctg ccgagacgct gccgccggcc gcccgggtct atctgcgtcg gcatgaccgg
      421 atgccgcgcg gcggctggtg ggcgcgcatg cggcaggagg tgctggttgc gctaccgcgc
      481 aactttcctc ttcaggccgt agagcgggaa gcggacccgc tcagggctgc gcgccgtcat
      541 agccctcgat gacgatcagg tcggcaatgc cgcatccgtc gcgcagcgcc ttggccgcct
      601 ggtattcctc cgaccggtag caggccagcg cggtgtcgta gtcggaaaac tccagcacga
      661 tgttgcgtgc gcgaggcgtc ccctccggcg cttccatgcg gccgccgcgc acgacgaaac
      721 gtgcgccgaa cttgcggaat gccgcggcgt tggccgccac atagcgctgg tagccggtgg
      781 ggtcggtgac atccacgcgc gcgatccaat aacccttggc catctgcatc ctcctggttc
      841 aatggaccca ctgtgcgcgc cgctccggct gcggcaaagt cctgcggtgc agaaggagtc
      901 ccgccatgcc cgacggcagg ctgatcatcg gcaaccgccg ctattcctcc tggtcgatgc
      961 gcggctggct ggcggtccgg ctggcgcaac tggacgtgga ggaggtgctg atccccttcg
     1021 agcgtcccgg tcccaccgcg gccatcggcg cggtctcgcc caacgggctg gtgccctacc
     1081 tggagcacaa gggcgcgcgt gtctgggagt cgctcgcggt gtgcgagtac tgcgccgagc
     1141 agaaggcggg cctgtggccg gagaaccggg tcgcacgggc catggcgcgc tctgtcgcgg
     1201 cggagatgca tgccgggttc cgcgagttgc ggcaggcgat gtggatgaac ctcgggtgcg
     1261 acttcgccgg ccacggccgc acgccgggcg cgctggccga tatcgcgcgg atcgaggcgc
     1321 tgtgggccga agcgcgccgt gcctatggca cgggcggccc gttcctgttt ggtgcggcgt
     1381 tcaccgcggc ggacgtgatg tacgcgccgg tggtggcgcg gttcctgacc tggcaaccgg
     1441 aactgtctcc cggagcccgc gcctattgtg acgcggtccg cgcgcatccg ctggtggctg
     1501 cctggtatga tgccgcggca cgagaaccgg ccgaatggtt gctgcccgac tatgagacgc
     1561 cgcgatgagc tgcgggaatg ctgccggcga gggatccctc agaacctgat ccgaaccccg
     1621 ccataggcag cgatcggcgc ggcgggagcc aggctgcggg tgtcggtggc gttcggtgcc
     1681 ttggcgtatg gcaccaggct ggtcggcgaa taggtgccat aggtggcgta ttgcgcgttc
     1741 agcaggttct gcaccaggcc gaacacctcc acgttggggg tgatgtgata ctggctgttg
     1801 atgttgagca cgacatacgg actggtcttc ggcgtcaggt tggcctcgtc gccgaacagg
     1861 tactgcccgc tgctgaccac gccggagaac ccgaccgtcc aggcctttgt caccgcatag
     1921 ctgacaccga atttgaaccg atgcgccggc acgccgggaa gccggtcgcc ggtcacgaca
     1981 tggatttctc ccgccgggtc cgcggccgga ttcaggggac tgaacagcgt ctgtccggtc
     2041 tggaaggttg cttcggtgaa ggcgtaattc gcccaggcgt gcagccggga gtcttgccag
     2101 gtgatgccag cctcgatccc ctgccgacgc gtgcggcccg cgttctggaa atacgccagg
     2161 cccggcacgg aactggcgac gaacaggata tcgtcgtcgc tgttggtgcg gaacaggccg
     2221 aacttccagt ccaatgtcgc cccggtcgcc gggtgcagca tgccgcgcag cccggcctca
     2281 acagtgtgtg ccaccacctg cttcaggtcg ggatcgccgg tgaagaaatt cgccagagta
     2341 cacgggcttt gcgggctggc gcacgacagt tcggccggcg tcggtgcgcg gttggcttcg
     2401 gaatagctgg cataaaccga gacctgcggc accagccggt aggtgatccc gatgcccgga
     2461 ttgaaatgcg tgtagctgtg gttgccggtg agcgcggagc cgatccggtc ctgcagatcg
     2521 atctgcgcca cgttcatgcg cccggcgagt gacagcgcga ggcgcggcgt taggtcgaac
     2581 acgtccgcga cataggcgcc gtaataggtg ttggtgctgt ccagccgtac cggcgccacc
     2641 ggcccgttcc gttgatcgat ggtgattccc ggtccgatga acacgccgtt gtcgctggtg
     2701 atgccgccga gtacgctgct cgcggtgtag gttgagatac cgcggtcgat actgatgccg
     2761 accaccagca ggttgtgccg tccggaaatg cgcgagtcat gcgtcaactg cagcgacgcg
     2821 ccatagccgt tgctgtccac cgcttccaga ttgagctgcg aatatgggcc gccattcaga
     2881 aaatcggtga taggatttcc ggcgcggtcc agcaggggat tgccttcgtc gtcgcacagc
     2941 gtgttgcctg cgggcgtgca gggagacgcg gcgaacgcat tgccgttcag gatccgctgc
     3001 gacaggttgg tgtagtagat gagcccctgc agcgatgcgc cgtagccgtt gctgtccacc
     3061 gcttccagat tgagctgcga atagggaccg ccgttcagga aattggtgat cggattgccc
     3121 gcgcggtcaa gcagcttgtt gccttcatcg tcgcacagtg catcgctctc cggtgcgcag
     3181 ggcaaggcgc cgaacgcgtt gccgttcagg atccgctgcg aca
//
LOCUS       DASSZH010000445         2008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_146798, whole genome shotgun sequence.
ACCESSION   DASSZH010000445 DASSZH010000000
VERSION     DASSZH010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146798"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VFN42_09660"
     CDS             <1..690
                     /locus_tag="VFN42_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET8996922.1"
                     /translation="GAGPHFCAGADLSEYSTQRGPEYRGRQEIDDDVWRLEQGQRLRI
                     ALFDMHKPVIAQVHGTCIGIGTELAWFCDMVIVADDARIGFPPVRDLGTPPGSMWLYH
                     CGPQWAKRLLLTGDSLTGKDAAAIGLALKSYPAEELQAEVMRLADRMGLVDPHLLSAN
                     KRMVNLGLELMGARTLQRIGAEIDARGHLAPKARAFRETMQQHGVKYAFQQRDAAFGS
                     GYAAVHGRDPD"
     gene            781..1554
                     /locus_tag="VFN42_09665"
     CDS             781..1554
                     /locus_tag="VFN42_09665"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8996923.1"
                     /translation="MSRRITVSGVELEVESHGAGRPLLFLHPGEGLWPRRPWLDALAA
                     KFRVIAPHHPGWGNSTLPDWMGTVDDLAYLYLDLARDLDLHDAVLVGACFGGWVAAEI
                     AVRNTQRFASLVLADPLGIKAGGVSDRDIADMHAMTQAEFLRHAWADPARGAVDYTAL
                     SEDQLAAIVRSREAFALFGWKPYMHNPRLKRWLHRIDRPTLLLWGEQDRIVTPAYGEA
                     WYAEIPGARMQLIRDAGHFPHWEQPAEFADRVAAFAQQH"
     gene            1568..>2008
                     /locus_tag="VFN42_09670"
     CDS             1568..>2008
                     /locus_tag="VFN42_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996924.1"
                     /translation="MRCWYFSEMAYHPAWDEGLKRGSLRVVLPNGNVDPHIAHKLLNR
                     YLDEFALCDEVGLDIMVNEHHSTATCMTVSAPMALAVIARETKRARLLTLGNPIANRP
                     DPVLPRIAQREWMSAGRNASPHLRHIVQRYLRPIFLKVSEVLREG"
BASE COUNT          337 a          639 c          702 g          330 t
ORIGIN      
        1 ggcgccgggc cgcatttctg cgccggcgcg gatttgtccg agtattccac ccagcgcgga
       61 ccggagtatc gcggacgcca ggagatcgac gacgatgtct ggcggctgga gcaaggccag
      121 cggctgcgga tcgcgctgtt cgacatgcat aagccggtga tcgcccaggt gcacggaacc
      181 tgcatcggca tcggcacgga gttggcctgg ttctgcgata tggtgatcgt tgccgacgac
      241 gcgcggatcg gctttccgcc cgtgcgcgac ctgggtacgc cgccgggatc gatgtggctg
      301 taccactgcg gcccgcaatg ggcgaagcgg ctgctgctga ccggcgacag cctgaccggc
      361 aaggatgcgg cggcgatcgg gctggcgctg aaatcctatc cggcggagga gctgcaggcc
      421 gaggtcatgc ggctggccga ccgcatgggg ctggtcgatc cgcatctgct gtccgccaac
      481 aagcggatgg tgaatctggg gctggaactg atgggcgccc gcacgctgca acgcatcggt
      541 gcggagatcg atgcccgggg gcatttggca ccgaaagccc gggcgttccg cgagacaatg
      601 cagcagcacg gggtgaagta cgcattccag cagcgcgacg ccgcgttcgg cagcggatac
      661 gccgcggtgc atggacgcga tccggactga cgcttgccat cccgccggcc cgcgcgttga
      721 attcccggcc aggaggaaac catccatgcg atcgccaccc tgcgcatcgg cgtgccagcg
      781 atgagccgcc gaatcaccgt ctccggcgtg gagctggaag tcgaatcgca cggcgcggga
      841 cgtccgctgc tgttcctgca tcccggtgag ggattgtggc cgcggcgtcc ctggctggac
      901 gcgctggcgg cgaagttccg ggtgatcgcg ccgcatcatc ccggctgggg gaattccacg
      961 ctgccggact ggatgggaac ggtggacgac ctggcctacc tgtacctcga cctggcgcga
     1021 gacctggacc tgcatgacgc ggtgcttgtg ggcgcctgct tcggcggctg ggtcgcggcg
     1081 gaaatcgctg tgcgcaacac gcagcgcttc gccagcctgg tgctggccga tccgctcggc
     1141 atcaaggccg gcggagtcag cgatcgcgac atcgccgata tgcacgcaat gacccaggcc
     1201 gagttcctgc ggcacgcgtg ggctgatccg gcacgcggcg cggtggatta taccgcgctg
     1261 tccgaagacc agttggcggc catcgtgcgg agtcgcgaag ccttcgcgct gttcggctgg
     1321 aagccgtaca tgcacaatcc acggctcaaa cgctggctgc accgcatcga ccggccgaca
     1381 ttgctgctat ggggcgagca ggatcggatc gtcacgccgg cctatggcga agcctggtac
     1441 gccgaaattc ccggcgctcg catgcagctg atccgtgatg cgggccactt cccgcattgg
     1501 gagcaaccgg cggaattcgc cgaccgcgtc gccgccttcg cgcaacagca ctgacgggca
     1561 ggaagcaatg cgctgctggt atttctcgga aatggcctat caccccgcgt gggatgaagg
     1621 cctgaagcgc ggctcgctcc gggtggtgct gccgaacggc aatgtcgatc cacatatcgc
     1681 gcacaagctg ctgaaccgct acctcgacga gttcgcgctg tgcgacgagg tggggctcga
     1741 catcatggtg aacgagcacc acagcacggc cacctgcatg acggtatcgg cgccgatggc
     1801 gctggcggtg atcgcgcgtg agaccaagcg tgcccgcctg ctgacgctgg gcaatcccat
     1861 cgccaaccgg ccggacccgg tgctgccgcg catcgcgcag cgggagtgga tgagcgcggg
     1921 acgcaacgct tccccgcacc tgcgacacat tgtgcaacgc tatttgcggc cgatttttct
     1981 gaaggtcagc gaggttctgc gggaagga
//
LOCUS       DASSZH010000446         3025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1698345, whole genome shotgun sequence.
ACCESSION   DASSZH010000446 DASSZH010000000
VERSION     DASSZH010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1698345"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VFN42_09675"
     CDS             <1..747
                     /locus_tag="VFN42_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET8996925.1"
                     /translation="DLIRIVGGLMKRRDKFDGIIIETTGLANPAPVAQTFFVDEGVRA
                     RTRLDAVVTVVDAKNLPARLEDSHEAEDQIAFADVIVLNKTDLVTPEELEEVERRIRA
                     INKFATIHRTQRAALPIAQVLDRGAFDLNRILSFDPDFLEDDSHEHNEDVASMSFEVQ
                     KPIDPEKFNAWIGLLLQEKGQDLLRTKGILAYAGDDRRFAFQAVHMMADGDFIGPWKD
                     GEPRVSRIVFIGRNLNRPQLRRGFEGCQAD"
     gene            805..1212
                     /locus_tag="VFN42_09680"
     CDS             805..1212
                     /locus_tag="VFN42_09680"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8996926.1"
                     /translation="MQIQPYLFFDGRCQEAIDFYRQALGAEVQMLMRFKECPQPTSVP
                     PGSDEKVMHAALKIGDATVLVSDGRCTGAPAFQGFALALNATDDAHARRMFDALSDGG
                     QVQMPLASTFFATSFGMVNDRFGITWMVIRQIG"
     gene            1334..2389
                     /locus_tag="VFN42_09685"
     CDS             1334..2389
                     /locus_tag="VFN42_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HET8996927.1"
                     /translation="MSQTESTPFLLRTDRGTQRELGAFVVAARFSRDSRHLAFALGDG
                     TLHLVALADRADWRATPVHDGAALALATDVTPNGFISGGDDGGFRRVGADGAVSDIAG
                     YGMKWVEQAASFAGDKGKGSLLACAVGKLVHLFDAAGKKVKEFAHPSSVTGLAFDAKG
                     KRIGASHYNGASLWFVGAKTDNPRKLEWKGSHTGIAIHPDGDAVVTAMQENALHGWRL
                     SDGQHMRMSGYPAKSESLSFSRNGKWLATSGADAMVLWPFFGGGPMGKAPVELAGGDG
                     VLCQRVACNPKEEIVAGGFADGLVVVADIDSSRVLPVAPAQHGPVSALAWSADGSQLA
                     FGTETGFAAIVDLSRRA"
     gene            2400..2588
                     /locus_tag="VFN42_09690"
     CDS             2400..2588
                     /locus_tag="VFN42_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996928.1"
                     /translation="MFGPVSDADLRAAQILTGAAMALWLGIRYVPGLQQHAVRAQVAV
                     LLLYLVGCVVVLAHALLR"
     gene            2667..2981
                     /locus_tag="VFN42_09695"
     CDS             2667..2981
                     /locus_tag="VFN42_09695"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET8996929.1"
                     /translation="MFIAMNRFKVVPTACTDFEQVWTRRDTYLQDVPGFIVFHLLRGP
                     ERDDHVLYSSHTLWRSREDFEAWTRSEQFRLAHKDAGQNKPLYLGHPEFEGFEVIQTV
                     KP"
BASE COUNT          495 a          988 c         1051 g          491 t
ORIGIN      
        1 gacctgatcc gcatcgtcgg cgggctgatg aagcggcgcg acaagttcga cggcatcatc
       61 atcgagacca ccggcctggc caatcccgcc ccggtcgcgc agacgttctt cgtcgatgaa
      121 ggcgtgcgtg cgcggacgcg gctggatgcg gtggtcaccg tggtggacgc caagaacctg
      181 ccggcacggc tggaggacag ccacgaggcg gaagaccaga tcgccttcgc cgatgtcatc
      241 gtgctgaaca agaccgacct ggtcacgccg gaggagctgg aggaggttga acgccgcatc
      301 cgcgcgatca acaagttcgc caccatccac cgcacccagc gcgccgcgct gccgatcgcg
      361 caggtgctgg accgcggcgc cttcgacctc aaccgcatcc tgagcttcga tcccgacttc
      421 ctggaagacg actcgcacga gcacaacgag gacgtcgcct cgatgagctt cgaggtgcag
      481 aagccgatcg atccggagaa gttcaacgcc tggatcggcc tgctgctgca ggagaagggc
      541 caggacctgc tgcgcaccaa gggtatcctg gcctatgccg gcgacgaccg ccgcttcgcc
      601 ttccaggcgg tgcacatgat ggcggacggc gacttcatcg gtccatggaa agacggcgag
      661 ccacgcgtca gccgcatcgt gttcatcggc cgcaacctga accgtccgca actgcgccgg
      721 gggtttgagg gctgccaggc ggactgagcc gccgggccat cgcatggccg cggatgtaag
      781 cggccggacc gggaaggaac ccgcatgcag atccagccct atctgttctt cgacggccgc
      841 tgccaggagg cgatcgactt ttatcgccag gcgctcggcg ccgaggtgca gatgctgatg
      901 cgcttcaagg aatgcccgca gcccaccagc gtgccgcccg gcagcgacga gaaggtgatg
      961 catgcggcgc tgaagatcgg cgacgcgacg gtattggtct cagacggccg ctgcaccggc
     1021 gcgccggctt tccagggatt cgccctggcg ctgaatgcca ccgacgatgc ccatgcgcgg
     1081 cggatgttcg atgcgctgag cgatggcggc caggtgcaga tgccgctggc cagcaccttc
     1141 ttcgccacca gctttggcat ggtgaacgac cggttcggca tcacctggat ggtgatccgg
     1201 cagatcgggt gaaccgcggc ggcgttccgc cgcagggttg acttccgacc ggcaacaggg
     1261 ctggtccgca tgctgtgggc cagccctgct gcgccgcaac ccgcgactcg acctgccaga
     1321 ccggaccgat tgcatgagcc agaccgaatc cacgcccttc ctgctgcgga ccgatcgggg
     1381 cactcagcgt gagctgggcg ccttcgtggt ggcggcgcgc ttctcccgcg acagccggca
     1441 tctcgccttc gcactgggcg acggcacgct gcatctggtg gccctggcgg atcgcgccga
     1501 ttggcgcgca acgccggtgc atgacggcgc ggcgctggca ctcgccacgg acgtaacgcc
     1561 gaacggcttc atctccggcg gtgacgacgg tgggttccgc cgggtgggcg ccgatggcgc
     1621 cgtcagcgac attgccggct atggcatgaa atgggtggaa caggcggcga gcttcgccgg
     1681 agacaagggc aagggcagtc tgcttgcctg cgcggtcggc aagctggtgc acctgttcga
     1741 tgccgcgggc aaaaaggtca aggagttcgc gcatccgtcc agcgtcaccg ggctggcgtt
     1801 cgatgccaag ggcaagcgga tcggcgcgtc gcactataac ggtgcctcgc tgtggttcgt
     1861 cggggcgaag accgacaatc cgcgcaagct ggaatggaag ggcagtcaca ccggtatcgc
     1921 catccatccc gatggcgatg cggtggtgac cgcgatgcag gagaatgcgc tgcatggctg
     1981 gcggctctcc gacgggcaac acatgcgcat gagcggctat ccggcgaagt ccgaatcgct
     2041 gtcgttcagc cgcaacggca aatggctggc caccagcgga gccgatgcga tggtgctgtg
     2101 gccgttcttc ggcggcgggc cgatgggcaa ggccccggtg gagctggccg gcggtgacgg
     2161 cgtgctctgc cagcgggtcg cctgcaatcc gaaggaggaa atcgtcgccg gcggcttcgc
     2221 cgacgggctg gtggtggtgg ccgacatcga ttcctcacgc gtgctgccgg tggcgccggc
     2281 gcagcatggg ccggtgtcgg cgctggcctg gagcgcggat ggcagccagc tcgcgttcgg
     2341 caccgagacc ggcttcgccg cgatcgtcga tctgtcgcga cgcgcatgac ggcgcgctga
     2401 tgttcggccc ggtcagcgat gcggatctgc gggcggcgca gatcctcacc ggagcggcga
     2461 tggcactgtg gctgggcatc cgctacgtgc ccgggctgca acagcacgcg gttcgcgcgc
     2521 aggtggcggt gctgctgctg tacctggtcg gctgcgtcgt ggtgctggcg cacgcgctgc
     2581 tgcgatagac cccgccgggg cgggcgttgg ccaagcccgc gtcagcccgg ctaatctcca
     2641 ccccttgcca tccgaggagc cgcagcatgt tcatcgcgat gaaccgcttc aaggtcgtcc
     2701 ccaccgcctg cacggatttc gagcaggtct ggacccggcg cgatacctac ctgcaggacg
     2761 tgcccggctt catcgtgttc catctgctgc gcgggccgga gcgtgacgac cacgtgctgt
     2821 actcgtcgca cacgctgtgg cgcagccggg aggatttcga ggcctggacc cgctcggaac
     2881 agttccgcct ggcgcacaag gatgccgggc agaacaagcc cttgtacctc ggccaccccg
     2941 agttcgaggg gttcgaagtg atccagactg tcaagccctg aacgccttca tgtcatgcgc
     3001 ggacctggtc cgcgcaccct ttttg
//
LOCUS       DASSZH010000447         7735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1595789, whole genome shotgun sequence.
ACCESSION   DASSZH010000447 DASSZH010000000
VERSION     DASSZH010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7735
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595789"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VFN42_09700"
     CDS             complement(<1..509)
                     /locus_tag="VFN42_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996930.1"
                     /translation="MFDLVIRGDTVVTPQAVGAFDIAIAGEKIVAVAASGSLPVPDSA
                     RVIDATGKIVMPGGIDPHVHCKWFLPNPDGSAGLTDPPDVVSKAAVHGGTTTIIDFTR
                     ASQGDNLRDAIEKREADWQGHCACDYAQHIMVEGALPVDLPPQLAEAIQAGFPTVKIF
                     TTDITPSRKG"
     gene            complement(796..987)
                     /locus_tag="VFN42_09705"
     CDS             complement(796..987)
                     /locus_tag="VFN42_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996931.1"
                     /translation="MTSISKRWAWVAFGMIDAVLLFDPGSAFAAPGAVPDIPQHLPAV
                     LLCGLGLLVTSMTGHRIRR"
     gene            complement(1153..1890)
                     /locus_tag="VFN42_09710"
     CDS             complement(1153..1890)
                     /locus_tag="VFN42_09710"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HET8996932.1"
                     /translation="MNRFDYHMVDAERGYGVGFNLLERSCYDSDEVNLVTQLLHLRRQ
                     YFGDGVVALDCGANIGVFTVEWARAMTGWGQVVAIEAQERVYYALAGNIAINNCFNAR
                     ALHAAVDAEPGELRIPRPNYLAPGTFGSLELRYNPETEYIGQPIDYSEQNMVPVRALS
                     IDSLGLPRLDLLKIDVERMELAALSGAQQTIRRHLPIIIIERLKSDEAQLHAVLEDYG
                     YRRFGIGMNLLAIHPSDRSCEHVKREP"
     gene            complement(2100..2879)
                     /locus_tag="VFN42_09715"
     CDS             complement(2100..2879)
                     /locus_tag="VFN42_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET8996933.1"
                     /translation="MTPVVGIPACAKLVDGQVRHDTPARYAAAAFGGSGVLPIMIPPL
                     GEAQIALLDRLDGLLLSGSPSNVHPSHYAGGDSETPDMHDLDRDVTTLRLIPEAIARG
                     MPILAICRGIQELNVALGGTLHQRVHLCAGRMDHRAGPGTLEEKYSPKHLVSLRGSVA
                     RMVGASTILVNSLHGQAIDQPAPGLVVEATAPDGTIEAVRLERLPGGAGAGTADGSLP
                     WVLGVQWHPEWGYANDSASLAIFRAFGDACRAYRTTLRQAA"
     gene            complement(2907..4103)
                     /locus_tag="VFN42_09720"
     CDS             complement(2907..4103)
                     /locus_tag="VFN42_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HET8996934.1"
                     /translation="MSLTLPRSPTTPRWLPVLLGYFIAVGPAATDMYLPAFPAVEQSF
                     GTEPGTAQLTLAAWFAGLALGQITQGTLSDRFGRRAPLIAGTGLFTLAAIGCALAPSL
                     GWLAAFRTLGAVGASAGMVIPRAMVRDLADGQSAAILMSRLMLVMGVAPILAPSIGGV
                     VLVFAQWRVIFWILTAYGAIGCFMAWRLLPETLLPPRRIHLRLAEQMHRYATIVRERS
                     FLTHAAMGASGTFCMFSYLAGSSPVFIDGFGLTPSEYALIFGLCSCGLIAGSQINARL
                     LPRFGLPFMLRLIARISLAATATLTVLAFTGVHVLPLIVAPVFVALSCQGFSNGNSSV
                     GALSRHAGHAGSAAALMGMAQFTLGAASGLLAGLFTDGTPRGMAALMLCGSVGAVIAD
                     LLRPRT"
     gene            complement(4244..5380)
                     /locus_tag="VFN42_09725"
     CDS             complement(4244..5380)
                     /locus_tag="VFN42_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET8996935.1"
                     /translation="MPDYTPYAADTLVIDMVRKYAQERLAPTAAAREKAGAIEPEVVA
                     ELGELGVFGATTPAQWGGSEIDPVTYALLLEEIAAGDGAISTMVSVHNSPTCIVLDHH
                     GTDAQKERWLRKLATGEHVGSFGLTEPQAGSDASNLRTRAVKRGDRYVINGAKQFISN
                     AALPGSIVFFAVTDPSAGKRGLSCFVADKTTPGFSIVRKEEKLGQKASDTCALAFTDM
                     ELPEDQRIGAEGEGYKIALSTLESGRIGIAAQSVGMARAALEFAVQYAKERTAFGQKI
                     MDFQAVGFRLVEAKTKLEAARQMTLHAARLKMEGKPALEAACMAKLFASEIAEAVCTA
                     AIQTLGGYGFLADFPVERIYRDVRVCQIYEGTSDVQKLILQRML"
     gene            5502..6743
                     /locus_tag="VFN42_09730"
     CDS             5502..6743
                     /locus_tag="VFN42_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HET8996936.1"
                     /translation="MRGLLGFVALLALAWLCSEDRRGIQWRTVVAGVALQILLAMLLL
                     GVPPATHAVFALNDAATALQNATDAGSRFVFGYLAGPPLPFAESHPGASFILAIQALP
                     LVLTISALASLLFYWGILQRITGAFAWLLRRVMGIGGALALGAAVHVFVGMIEAPLLI
                     RPYLARMQRGELFALMTCGMAGVAGTVMVIYAGFLEPVIPNALGNILIASVISTPAGL
                     AVAALMVPFVAVDAAEETRLEMRDPPLSSLDALVKGTLEGIPILAGIIAVLLVAIASV
                     ALLNSMLALLPHWGGAPVTLQAIFALPFRPVMWLLGVPWRDSATAASLMATKTVLNEF
                     VAYLNFSHIPAATFAPRTRLILTYALCGFANFGSLGIMIGGMGAMVPERRHEIVALGL
                     RTILSGTIATCMSGALAGMLA"
     gene            6765..7622
                     /locus_tag="VFN42_09735"
     CDS             6765..7622
                     /locus_tag="VFN42_09735"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HET8996937.1"
                     /translation="MNWTDRRNRYRALLAGAHCIHPGSVFDPISGRIAEELGFEVGMF
                     AGSIASFTVLGAPDIIVLTLTEFAQQVYRINRACRMPVMVDADHGYGNALNVMRTVQE
                     LETAGVAALTIEDTNLPRAFGEKQPTLIPIEEGVGKMRAALAAREDPSLTIIGRTSAT
                     GIAGTEEAVRRAKAYAETGVDALFFAGGMTRQQLDAIAEAVTLPLMLGGVGPELQDRD
                     YLASRNVRIALQGHQPFSAAVKAVYETLKALRDGVQPKDLQGIAASEMMARVTREGDY
                     KSWTERFLL"
BASE COUNT         1246 a         2572 c         2645 g         1272 t
ORIGIN      
        1 cccttgcggc tgggcgtgat gtcggtggtg aagatcttca ccgtggggaa tccggcctgg
       61 atcgcttccg ccagttgcgg cggcagatcg accggcagcg cgccttccac catgatgtgc
      121 tgggcatagt cgcaggcgca gtggccctgc cagtccgctt cgcgcttttc gatcgcgtcc
      181 cgcaggttgt cgccctggct ggcgcgagtg aagtcgatga tcgtcgtggt gccgccatgc
      241 accgccgcct tgctcaccac gtcgggcgga tcggtcagac cggcgctgcc gtccggatta
      301 ggcaggaacc atttgcagtg cacatgcggg tcgatgcccc cgggcatgac gatcttcccc
      361 gtggcgtcga tcacccgggc actgtccggc accggcaggc tgccgcttgc cgccaccgcg
      421 acaatctttt cgccggcaat ggcaatatcg aacgcgccga cggcctgcgg cgtgaccacg
      481 gtgtcgccgc ggatcaccag atcgaacatg acgtttctcc ccagcagcag gcgggcatgg
      541 ttccgcgtta cccgctgcag ggcaaggggc agtcaggccg cgtcataccg gcgggcgaaa
      601 tcgaggggat cctgctggcc gcccgcgccg ccgccaggat gctcgccgcc tgctccagca
      661 cgacggggcc cgggattccg cgaggaggag gggagggtgg acaacgggcg agcgcacccg
      721 atttcccgct tcgccttcgg aatggcgcaa cgggagcacg gcgttgcatc tggcggcaag
      781 tgccgtccgg ccgtgctagc ggcggatgcg atgtccggtc atgctggtta ccagcaggcc
      841 cagtccgcac agcagaaccg cgggcagatg ctggggaatg tcgggaaccg cgccaggcgc
      901 ggcaaaggcg gaaccgggat cgaacagcag cacggcgtcg atcatgccga aggcgaccca
      961 ggcccaccgc ttggagatag atgtcatggc ccaaccctcc ttttggatgg ctgggtagca
     1021 cgcccggttt tgcacggcgg ttgccgggcg gccgaatacg gcgggggatg gcccggtctt
     1081 tgtcacgccg ccttcactcc cgcaatacga tccggggcag gcggcagcgt ctgcccaccc
     1141 cggcctggct tgtcagggtt cccgcttgac gtgttcgcag gacctgtcgc ttgggtggat
     1201 cgccagcaga ttcattccaa taccaaagcg acggtagccg tagtcttcaa gcacagcatg
     1261 cagttgggct tcgtcggatt tcaatcgttc gataatgatg atcggaaggt gccggcgtat
     1321 ggtctgttgc gccccggaca atgcggccaa ttccatgcgc tcgacatcga tcttgagcag
     1381 gtccagccgc ggcagcccaa gtgaatcgat gctaagggcg cgcaccggta ccatattttg
     1441 ttcggaataa tcgatcggct ggccgatgta ttccgtttca gggttgtagc gcagttccag
     1501 actgccgaac gtgcccggcg cgagatagtt ggggcgcggg atacgcagct cgcccggctc
     1561 cgcatccacg gcggcgtgca atgcgcgcgc gttgaagcag ttgttgatcg ctatgttgcc
     1621 ggccagcgcg tagtacaccc gttcctgcgc ctcgatggcg accacctggc cccagccggt
     1681 cattgccctg gcccactcca cggtgaacac gccgatattg gcgccgcaat ccagcgccac
     1741 tacgccatcg ccaaaatact ggcggcgcag atgcaacagc tgggtgacaa gattgacctc
     1801 gtcggaatcg tagcacgacc gctcgagcag attgaacccg acgccgtaac cacgttcggc
     1861 gtctaccatg tggtagtcga accggttcac gatcagcgtc ccgtgattgg tggatgcaag
     1921 cacgcacccg attttgcgcg gaggaaatgt catgtgaccg ccccgaaatg aggctggcag
     1981 gaatatcagg tcgcagaagc cgtgtcccgg gccgaccgca aggcccgttc caatcttcgg
     2041 agaatccccc agcgcagcct gagcctgtcc gcctcaagac gggaagcgga cagccctggt
     2101 caggcggctt gccgcaatgt cgtccgatac gcccggcagg catcgccgaa ggcgcggaag
     2161 atcgccaggc tggccgaatc gttggcgtat ccccattccg ggtgccattg cacgccgagc
     2221 acccagggca gcgacccatc ggcagtgccg gccccggcgc cgccgggcag gcgttccagc
     2281 cgcaccgcct cgatcgtgcc gtccggtgcg gtggcttcca cgaccaggcc tggtgccggc
     2341 tggtcgatcg cctggccatg cagcgaattc accaggatcg tcgaggcacc gaccatgcga
     2401 gccacgctgc cgcgcaagga gaccaggtgc ttcgggctgt acttctcctc cagcgtgccg
     2461 ggaccggcgc gatgatccat ccgtccggcg cacagatgca cgcgctggtg cagcgtgccg
     2521 ccgagcgcga cgttgagctc ctggatgccg cggcagatcg ccaggatcgg catgccgcgg
     2581 gcgatcgcct cggggatcag ccgcagcgtg gtgacgtcgc ggtccaggtc gtgcatgtcc
     2641 ggggtttcgc tatcgccccc ggcatagtgg ctgggatgca cgttgctcgg gctgccggac
     2701 agcagcagac cgtcaagccg gtcgagcagc gcgatctgcg cctcccccaa ggggggaatc
     2761 atgatcggca gcacgccgct gccgccgaac gccgccgcgg catagcgggc cggcgtgtcg
     2821 tgacgcacct gcccgtccac cagcttggcg caggccggaa tacccaccac aggggtcatg
     2881 cacgaatcct tcattgtact gcaccactat gtgcgcggac gcagcaaatc cgcaatcacc
     2941 gcgccaaccg agccgcacag catcagcgcc gccatgccgc ggggggtgcc gtcagtgaac
     3001 aggcccgcca gcaggccgct ggcggcgccc agcgtgaact gcgccatgcc catcagcgcc
     3061 gcggcgctgc cggcatgtcc cgcatgccgc gacagcgcgc ccaccgagct gttgccgttg
     3121 ctgaaaccct ggcagctcag cgcgacgaag accggcgcga cgatcagcgg cagcacgtgc
     3181 acgccggtga acgccagcac cgtcagcgtg gccgtggcag ccagcgagat gcgggcgatc
     3241 agccgcagca tgaagggcag gccgaagcgc ggcagcaggc gcgcgttgat ctgcgagccg
     3301 gcgatcaggc cgcaggagca caggccgaag atcagcgcgt attccgacgg cgtcaggccg
     3361 aacccatcga tgaacaccgg ggacgagccg gccaggtagg agaacatgca gaaggtgccg
     3421 gaggcgccca tcgccgcgtg ggtcaggaag ctgcgttccc gcacgatcgt cgcgtagcgg
     3481 tgcatttgtt ccgcgagccg cagatggatg cggcgtggcg gcagcagcgt ttccggcagc
     3541 agccgccagg ccatgaagca gccgatcgcg ccataggcgg tgaggatcca gaagattacc
     3601 cgccactgcg cgaataccag caccacaccg ccgatgctgg gcgccaggat cggcgccacg
     3661 cccatcacca gcatcagccg cgacatcagg atggcggcgg actggccgtc ggccaggtcg
     3721 cgtaccatgg cgcgcgggat caccatgccg gccgaggcgc ccaccgcccc caatgtccgg
     3781 aacgctgcca gccagccgag gctgggtgcc aaggcgcagc cgatcgccgc cagggtgaac
     3841 agcccggtgc cggcgatcag cggtgcgcgc cggccgaagc ggtcggacag cgtgccctgg
     3901 gtgatctggc ccagcgccag cccggcgaac cacgcggcca gggtaagctg ggcggtaccc
     3961 ggctcggtgc cgaaggactg ttccaccgcc gggaaggcgg gcaggtacat gtccgtggcg
     4021 gccgggccga cggcaatgaa atagccgagc agcacaggca gccagcgtgg cgtcgtcggg
     4081 gagcggggca gggtcagcga cattgccagc cggattctag cgccgatgct gcggctgcga
     4141 aacgcccgcg gtcccgctat gaaatctgcc ctggggcttg cacgcggcat ggccggcgaa
     4201 gcccctcgca gggaaacctg acggcgtgcc ggccgccttc aggctacagc atgcgttgca
     4261 ggatcagctt ctgcacgtcc gacgtgcctt catagatctg gcagacgcgc acgtcgcggt
     4321 agatgcgttc caccgggaaa tccgccagga agccgtagcc gcccagagtc tggatcgccg
     4381 ccgtgcacac cgcctcggcg atttccgagg cgaacagctt ggccatgcac gccgcttcca
     4441 atgcgggctt gccttccatc ttcagccgag cggcatgcag cgtcatctgc cgcgcggctt
     4501 ccagcttggt cttcgcctcg acgaggcgga agccgaccgc ctggaaatcc atgatcttct
     4561 ggccgaacgc ggtgcgctcc ttggcgtatt gcacggcgaa ctccaatgcc gcgcgcgcca
     4621 tgccgacgct ttgtgcggcg atgccgatgc ggccgctctc cagcgtggac agcgcgatct
     4681 tgtacccttc gccttccgcg ccgatccgct ggtcttccgg caattccatg tcggtgaagg
     4741 ccagcgcgca ggtatccgac gccttctgcc ccagcttctc ctccttgcgc acgatggaga
     4801 aacccggcgt ggtcttgtcg gcgacgaagc aggacaggcc gcgcttgccg gcggacggat
     4861 cggtgaccgc gaagaacacg atgctgccgg gcagcgcggc gttgctgatg aactgcttgg
     4921 cgccgttgat cacgtagcgg tcgccccgct tcaccgcgcg ggtgcgcagg ttggacgcat
     4981 cggagccggc ctgcggctcg gtcagcccga aggagccgac atgctcgccg gtggccagct
     5041 tgcgcagcca gcgttccttc tgcgcatccg tgccgtggtg gtcgagcacg atgcaggtcg
     5101 gggaattgtg taccgatacc atggtggaga tggctccgtc gccggcggcg atctcctcca
     5161 gcagcagcgc gtaggtgacc ggatcgatct ccgagccgcc ccactgcgcc ggggtggtgg
     5221 cgccgaacac gccgagttcg cccagttcgg cgacgacctc cggttcgatc gcgccggcct
     5281 tctcccgcgc cgccgcggtg ggggccagcc gctcctgcgc gtatttgcgc accatgtcga
     5341 tcaccagggt gtcggcggca tagggcgtgt agtcgggcat ggcggtcacc tgctggctga
     5401 agggtggcgc ccagcatacc ccgaatgctg cttgcctcac agcgcggggc ggcttggcgg
     5461 ggtcatgcgg tgtgggtaag aggggcggag ggggagcgct gatgcggggt ctgctcgggt
     5521 ttgttgcgct gcttgcgctt gcctggctgt gttccgagga ccgccgcggc atccagtggc
     5581 gcacggtcgt ggccggcgtg gcgttgcaga tcctgctggc gatgctgctg cttggcgtgc
     5641 ctcccgcgac gcacgcggtg ttcgcattga atgacgccgc caccgccttg cagaacgcca
     5701 ccgatgccgg atcgcgcttc gtgttcggct atctcgccgg cccgccgctg cccttcgcgg
     5761 aaagccatcc cggtgccagc ttcatcctgg cgatccaggc gctgccgctg gtgctgacca
     5821 tcagcgcgct ggcatcgctg ctgttctatt ggggcatcct gcagcgcatc accggtgcct
     5881 tcgcctggct gctgcggcgc gtcatgggca tcggcggcgc cctggcgctc ggcgccgcgg
     5941 tgcacgtgtt cgtcggcatg atcgaggcac cgctgctgat ccgcccgtac ctcgcccgca
     6001 tgcagcgcgg cgaactgttc gccttgatga cctgcggcat ggccggcgtc gccggcaccg
     6061 tgatggtgat ctatgccggc ttcctggagc cggtgatccc caacgcattg ggcaatatcc
     6121 tgatcgcctc ggtgatcagc acgccggccg gactcgccgt cgccgcgctg atggtgccct
     6181 tcgtggcggt ggacgcggcc gaggagacaa ggctggagat gcgcgatccg cccttgtcgt
     6241 cgctcgacgc gctggtgaaa ggcacgctgg agggcattcc gatcctggcc ggcatcattg
     6301 ccgtgctgct ggtcgcgatc gcatcggtgg ccttgctgaa cagcatgctc gccctgctgc
     6361 cgcattgggg gggcgcgccg gtgacgctgc aggcgatctt cgcgctgccg ttccgcccgg
     6421 tgatgtggct gcttggggta ccgtggcgcg attccgccac ggcggcgtcg ctgatggcga
     6481 ccaagacggt gctgaatgaa ttcgtcgcct acctcaactt ctcgcatatt ccggcggcca
     6541 ccttcgcccc gcgcacgcgg ctgatcctga cttacgcgct gtgcggcttt gccaatttcg
     6601 gcagccttgg catcatgatc ggcggcatgg gcgcgatggt gccggagcgg cggcacgaaa
     6661 tcgtcgcgct gggcctgcgc actatcctga gcggcacgat tgcaacctgc atgagcggcg
     6721 cgctggccgg catgctggcc tgaaccaagc ggaggacacg cagcatgaac tggaccgatc
     6781 gccgcaaccg ctaccgcgcg ctgctggcgg gcgcgcactg catccatccc ggttcggtgt
     6841 tcgacccgat ctccggccgc atcgcggagg agctcggctt tgaagtcggc atgttcgccg
     6901 gctccatcgc cagcttcacc gtgctgggcg cgccggacat catcgtgctg acgctgaccg
     6961 agttcgcgca gcaggtctat cgcatcaacc gcgcctgccg gatgccggtg atggtggatg
     7021 ccgatcatgg ctacggcaac gcgctgaacg tgatgcgcac ggtgcaggag ctggaaaccg
     7081 ccggcgtcgc tgcgctgacc atcgaggaca ccaacctgcc gcgcgcgttc ggcgaaaagc
     7141 agccgacgct gatcccgatc gaggagggcg tcggcaagat gcgtgcggcc ctggcggcgc
     7201 gggaagaccc gtcgctgacc atcatcggcc gcaccagcgc gaccgggatt gccggcaccg
     7261 aggaggcggt tcgccgcgcg aaggcctatg cggaaaccgg agtggatgcg ctgttcttcg
     7321 ccggtggcat gacgcggcaa caattagacg cgatcgccga agcggtgact ctgccgctga
     7381 tgctgggcgg cgtgggtccc gaattgcagg atcgcgacta tctggccagc cgcaacgtgc
     7441 gcatcgcctt gcaggggcat cagcccttct cggcggcggt aaaggccgtt tacgaaacgt
     7501 tgaaggcctt gcgggacggg gtgcagccca aggacctgca aggcatcgcg gcatccgaga
     7561 tgatggcgcg ggtcacccgg gagggcgatt ataaaagctg gaccgaacgg ttcctgctgt
     7621 agccattcgc gcggggcgtc gccagcgaaa gccgggccgt ggcccacgca tcagcgccgg
     7681 caaaggccgg cgccgacggc acgcagcaga ggcatgcgtg gcgggcctgc gcccg
//
LOCUS       DASSZH010000448         2184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_334505, whole genome shotgun sequence.
ACCESSION   DASSZH010000448 DASSZH010000000
VERSION     DASSZH010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334505"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2184
                     /locus_tag="VFN42_09740"
     CDS             <1..>2184
                     /locus_tag="VFN42_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HET8996938.1"
                     /translation="ARQLDIPAAAFADYAQRPQTMTDHVRLLAVMLGLRPAANTDLAM
                     MIETAAQAAWHTDRGHPIAAAVVTALRTAGIMLPAGSVIERAAIAGRARARKRATDAV
                     LTGVTGAQMAALDQLLTRDAAAGMTPLAWLKTMPVAPKPDHIRELLDRLRRVRDVGLS
                     PDIAAKIAEERWRQFVREGYASDAHQLGRLTAGRRRATLAATIIDLEARLTDAVLEMT
                     DKLIGGVFARARTATRTRYMASAGDVGRLMRLFDGTIAALAIAQADERDAFEVVDESV
                     GWARLLRVRGEVGDLAQLAEEDPLLRAADRWKTLRKFVPDLIAALEFRAARPGDPLLA
                     ALQLLADMHRSGKREIPADAPMPFRKNWRRLVMESGTANRRRYETAVLATLRNKLRSG
                     DIWVDRSSSYRRFDSYLLPASAVPAIAAKLGLPPTADEWLTTKGNELDRRLKDFALKL
                     QRGELQGVELRDGRLSVTPVKATTTPQAHAFADAIEAIMPRVRITELLHEVNRATGFA
                     AAFTNLRTGERCEDDNALLATILADATNLGLGRMAAASHGVTRDKLVWIADAHIRPET
                     YQAALTRIINAHHALPIAAIWGDGSTSSSDRQFFRSGKRGNTAGDVNARYGQDPGLGF
                     YTHVSDQHGPYSARVMSATSHEAPYVLDGLLHHGSALRIGTHYTDTGGASDHVFALCT
                     MLGIRFCPRLRDFPDRKFACVEPAATYKALQPLFGRRIRIDTIRDP"
BASE COUNT          382 a          757 c          704 g          341 t
ORIGIN      
        1 ggccagacag ctcgacatac cggcggctgc gttcgcggac tatgcgcaac gcccgcagac
       61 catgacggac catgtgcgcc tgctcgccgt catgcttggc ctgcggccgg cggcgaacac
      121 agacctggcg atgatgatcg agacggcggc gcaggccgcc tggcacacag atcgagggca
      181 ccccatcgcc gccgccgtgg tcaccgcgct gcgtaccgcc ggcatcatgc tgccggccgg
      241 cagtgtcatc gaacgagccg cgatcgccgg ccgcgcccgc gcgcgcaagc gtgcgacaga
      301 cgcggtgctc accggcgtaa ccggcgcgca gatggctgca ctcgaccagc tgctgacacg
      361 cgacgccgca gcgggcatga cgccgctggc ttggcttaag acaatgccgg tcgcccccaa
      421 gccagatcac atccgcgaat tactcgaccg gctgcggagg gtgcgtgacg tcggcctgtc
      481 tcctgacatc gctgcaaaga ttgccgagga aagatggcgg cagttcgtgc gcgagggcta
      541 cgccagcgac gcccatcaac tcggccgcct caccgcaggc cggcgccgcg caaccctggc
      601 cgcaaccatc atcgatcttg aggcgcggct gaccgatgcg gtgctggaga tgaccgacaa
      661 gctcatcggc ggcgtgttcg ccagggcgcg tacggccaca cggacgcgct acatggccag
      721 cgccggcgat gttggtcggc tgatgcgtct gtttgacggc acgatcgcgg cactggcgat
      781 agcgcaagcc gatgaacgcg atgccttcga ggtggttgac gagtcggtgg gctgggcgag
      841 actgctgcgc gtgcgtggcg aagtcggcga cctcgcccag ctcgccgagg aagacccgct
      901 gctgcgcgcc gccgatcgct ggaagacctt gcggaagttt gtacccgatc tgattgcggc
      961 actggagttc cgcgctgcac gtcccggcga tccgctgctg gcggcgctgc aactgctcgc
     1021 cgacatgcac cgctccggca agcgcgagat accggcagac gcgccgatgc cgttccgcaa
     1081 gaactggcgg cggctggtca tggaaagcgg cacagcgaat cggcgccgct acgagaccgc
     1141 ggtgctggcg acgctgcgca acaagctgcg ctcgggcgac atctgggtcg atcgctcgtc
     1201 cagctatcgt cgcttcgaca gctatctcct gccagcgtct gctgtcccgg ccatcgcggc
     1261 gaagcttggg ttgccgccca cggcagatga atggctcacg acgaagggca acgaactcga
     1321 ccggaggctg aaagacttcg cactgaagct gcaacgcggt gaactccagg gcgtcgaatt
     1381 gcgcgacggg cggttgagcg tgacaccggt caaggcaacc accacgccgc aggcccacgc
     1441 gttcgccgac gcgatcgagg cgatcatgcc gcgcgtgcgc atcaccgagt tgctgcacga
     1501 ggtgaaccgg gccaccggct ttgccgccgc gttcaccaac ctgcgcaccg gcgagcgctg
     1561 cgaggacgac aacgccctgc tcgccaccat tctggccgat gcgaccaatc tcggcctcgg
     1621 ccgcatggcg gcggccagcc atggcgtgac ccgcgacaag ctggtctgga tcgccgatgc
     1681 acatatccgc ccagagacgt accaggcggc actcacccgc atcatcaacg cgcatcacgc
     1741 attgccaatc gcggcgatct ggggtgacgg cagcacctca tcgtcggatc gccagttttt
     1801 ccgctctggc aagcgcggca acactgccgg cgatgtcaac gcgcgctacg gccaggaccc
     1861 aggtctgggt ttctatacgc atgtctccga tcagcacgga ccctacagcg cacgcgtcat
     1921 gtcggcgacc agccacgagg ccccatatgt gctggacggg ttgctgcatc atggctcggc
     1981 gctgcgcatc ggcacgcact ataccgacac gggcggcgcc tcggaccatg tgttcgctct
     2041 ctgcaccatg ctgggcattc ggttctgccc gcggctgcgc gattttcccg accgcaagtt
     2101 cgcctgcgtc gaacctgccg caacctacaa ggctcttcag ccactgtttg gacgacgtat
     2161 ccgcatcgat accatccgcg accc
//
LOCUS       DASSZH010000449         2657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_402682, whole genome shotgun sequence.
ACCESSION   DASSZH010000449 DASSZH010000000
VERSION     DASSZH010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402682"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VFN42_09745"
     CDS             complement(<1..314)
                     /locus_tag="VFN42_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996939.1"
                     /translation="MDARKTALPLSRRSILAGAAGLAATPLISTSALAAERCVVGTWG
                     GDYAKLLRENIDDPILKKEGVHVVQAIGDEAPRMAQLYAKRMLPRGSMDIACLSSFNG
                     YRA"
     gene            complement(317..1438)
                     /locus_tag="VFN42_09750"
     CDS             complement(317..1438)
                     /locus_tag="VFN42_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8996940.1"
                     /translation="MSEGATALELRALSKQFSGHLAVREVNLNVAPGEMIVLLGPSGC
                     GKTTTLRMIAGFLPASSGDILLDGNSIVGVPAYRRDIGIVFQSYALFPHLTVARNVRF
                     GLEMRRLAAAQANARVMEMLRLVKLDSFAERLPRQLSGGQQQRVALARALAIHPRLLL
                     LDEPLSNLDAALRQEMAREIRILQRDRGITAIVVTHDQSEAMAMADRLVVMRDGGVEQ
                     IGSQEDLYERPATPFVAGFIGQSNLLRGQLRNGDQLLTDGDGRIALARRYAGSGAACL
                     AVRPESIGLARPDAADARAMGTVMLATYLGAVVEYVVRLDSAIDVIVRSPGHGSAEQR
                     RAAGERVCLTWPQAAERLYDEHDRPAAAMPQTQTVTAGG"
     gene            complement(1435..2304)
                     /locus_tag="VFN42_09755"
     CDS             complement(1435..2304)
                     /locus_tag="VFN42_09755"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8996941.1"
                     /translation="MRSSGSQALASATLPLHHDRSELFGRIGRLVIAALILCGLLIIF
                     VPLLLTLYLSVFDQKLIAFPPPGYTLDWYPAILPNFGDAIVTSLELAIAAVIGSLMLG
                     IPAGIALSRHRFRGRGVISTLLLSPLTVPGIALGLAIYLFLVLIEIWTGWALTGSLPG
                     LILSHLVITMPWVVRLCVASLINHDRAAEEAATSLGARPLTVIWRVTLPAMRQGIIAA
                     ALFAFIFSFGNLEMALFLVAPGVTTLPVAILEYLEYHIDPLVAAVAVAQMILVGGLML
                     ILDRFVRLGQVVR"
     gene            complement(2294..>2657)
                     /locus_tag="VFN42_09760"
     CDS             complement(2294..>2657)
                     /locus_tag="VFN42_09760"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8996942.1"
                     /translation="SLAKNEIVLDAGLRSLEICREAGVEIGFGTDLLGQLQDDQCNEF
                     HIRAQALGAAAVIRSATLVNARILRQEGKLGELVPGAHADLLVVDGDPYRDLGVFQGE
                     GARIPAIMLGGRFVKHAL"
BASE COUNT          482 a          918 c          848 g          409 t
ORIGIN      
        1 gcgcgatagc cgttgaacga gctgaggcag gcgatgtcca tcgacccacg cggcagcatg
       61 cgcttggcat agagctgcgc catgcgcggt gcctcgtcgc cgatcgcctg caccacatgg
      121 acgccttcct tcttcaggat cgggtcatcg atgttttcgc gcagcagctt cgcgtaatcg
      181 ccaccccagg tgcccaccac gcagcgttcc gctgccagcg ccgacgtgga aatcagcggt
      241 gtcgcggcca atccggcggc gcccgccaat atgctgcgcc ggctgagcgg cagagcagtc
      301 ttccgtgcat ccattgttag cctcctgctg tcactgtttg tgtttgcggc atcgccgcgg
      361 cgggccggtc atgctcgtcg taaaggcgct ccgcggcctg tggccaggtc aggcatacgc
      421 gttcccccgc cgcacgccgc tgctcggcac tgccgtggcc gggactgcgc acgatcacat
      481 cgatggccga gtccagccgc acgacatatt cgacgaccgc gccaagatac gtggccagca
      541 tcacggtgcc catcgcgcgg gcatctgctg cgtcgggcct tgccaagccg atgctctccg
      601 gccgcaccgc aaggcaggcc gcgccggaac ccgcgtaccg ccgggcaagc gcgatccgac
      661 catccccgtc ggtcagcaat tggtcgccgt tccgcagttg gccgcgcagc aggttgctct
      721 ggccgatgaa gccggcgacg aagggagtcg ccgggcgctc gtacaggtct tcctggctgc
      781 cgatctgctc cacgccgccg tcgcgcatca ccaccagacg gtcggccatc gccatcgcct
      841 cgctctggtc atgcgtgacg acgatcgcgg tgatgccgcg gtcgcgctgc aggatgcgga
      901 tctcgcgcgc catttcctgg cgcagcgcag cgtccagatt ggacagtggc tcgtccagca
      961 gcagcaggcg cggatggatc gccagcgcgc gggccagtgc cacacgctgt tgctgccctc
     1021 ccgacaactg tcgcggcagc cgctcggcga aactgtccag cttcaccagg cgcagcatct
     1081 ccatcacccg cgcattggcc tgcgccgccg ccaagcgccg catctccagt ccaaagcgca
     1141 cgttgcgcgc caccgtcaga tgcgggaaca gcgcgtagct ttgaaacacg atgccgatgt
     1201 cgcggcgata ggccggtacg ccgacgatgc tgttgccatc cagcaggata tcgccggagg
     1261 aggccggcag aaatccggcg atcatgcgca gcgtggtggt cttgccgcag ccggaggggc
     1321 ccagcagcac gatcatctcg cccggcgcga cgttcaggtt cacctcgcgc accgcgagat
     1381 gaccactgaa ctgcttgctc agcgcgcgca gttccagcgc ggtggcgccc tcgctcatcg
     1441 caccacctgt ccaagccgca caaagcgatc aagaatcagc atcaggccgc cgaccaggat
     1501 catctgcgcc accgcgactg ccgcaaccag cgggtcgata tggtattcca ggtattccag
     1561 gatggcgacc ggcagggtcg tgacgcccgg tgcgaccaga aacaaagcca tctccagatt
     1621 tccgaaggag aaaatgaagg cgaacagcgc ggcggcgatg atgccctggc gcatcgccgg
     1681 cagcgtgacg cgccagatca ctgtcagcgg ccgagcgccg aggctggtgg cggcttcttc
     1741 cgcggcgcgg tcgtggttga tcaggctggc gacgcatagc cgcaccaccc agggcatggt
     1801 gatcaccagg tgcgacagga tcagtccagg cagcgagccg gtcagcgccc agccagtcca
     1861 gatctcgatc agcaccagga acaggtagat cgccagcccc agggcgatcc cgggcaccgt
     1921 cagtggtgac aacaacagcg tgctgatcac gccgcggcca cggaagcgat gacgcgacag
     1981 cgcgatgccg gcgggaatgc ccagcatcag gctgccaatc accgcggcga tggcaagctc
     2041 cagactggtg acgatcgcgt cgccgaaatt cggcaggatc gccggatacc agtccagcgt
     2101 gtagccgggc ggcgggaatg cgatcagctt ctgatcgaac acactgaggt acagcgtcaa
     2161 cagcagcggc acgaagatga tcagcaggcc gcacaggatc agggctgcga tgaccagccg
     2221 gccgatccgg ccgaacagtt ccgatcggtc gtgatgcagc ggaagcgtgg cgctcgccaa
     2281 tgcctgcgag ccgctagagc gcatgcttga cgaagcgccc cccgagcatg atcgccggaa
     2341 tgcgcgcgcc ttctccctgg aaaaccccaa gatcgcgata gggatcgccg tccaccacca
     2401 gcaggtcggc atgcgcgccc ggcaccagct cgccgagctt gccctcctgg cgcaggatcc
     2461 gcgcgttcac cagcgtggcg gagcgaatga ccgccgcggc acccagcgcc tgtgcgcgaa
     2521 tgtggaactc gttgcactgg tcgtcctgca actggccgag caggtcggta ccgaagccga
     2581 tttcgacgcc ggcttcccgg cagatctcca gcgagcggag gccggcatcc agtacaatct
     2641 cgttcttcgc caggctg
//
LOCUS       DASSZH010000450         3488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_436972, whole genome shotgun sequence.
ACCESSION   DASSZH010000450 DASSZH010000000
VERSION     DASSZH010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436972"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VFN42_09765"
     CDS             complement(<1..216)
                     /locus_tag="VFN42_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8996943.1"
                     /translation="MHLMYFTEQPMAAYDAKEGLKYGATALTFSNKHYDPVEGSRLYN
                     EYLEEYIMADEYGADGIMLNEHHNAPFC"
     gene            complement(290..1063)
                     /locus_tag="VFN42_09770"
     CDS             complement(290..1063)
                     /locus_tag="VFN42_09770"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8996944.1"
                     /translation="MSVAWSDTTHEIAGTRMHLSRAGSGRPLLILHHDIGTPDRLAFY
                     DALAGSYDVLVPHHPGWGKSERPNWLRHPRDIAAMHAWLLAELGLREVALVGLGFGGW
                     IAAEMASMAPAAYRKLVLAGPMGIKPPEGDIMDQAIVSYIDYPRAGFHDQAAFTRIYG
                     DVTTDQLEQWDICREMSFRTAWKPYMYSQTLPHLIGGIRAPALVVWGDDDRHVPASAA
                     PLWQHGLRNARLEMIGSCGHYVDMEKPDELARLVTGFVD"
     gene            1186..2742
                     /locus_tag="VFN42_09775"
     CDS             1186..2742
                     /locus_tag="VFN42_09775"
                     /EC_number="3.4.11.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011252791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminopeptidase"
                     /protein_id="HET8996945.1"
                     /translation="MSISTRLDQTLSQLPQRYPGPGGAVAVLRNGEILLRHAWGWGDA
                     ERRVAFTPRTLFRMCSITKQFTCALVLDAFPDPSVLDADVRARLPRLQQDAPGALHLC
                     HNQSGLRDYWAVAMLLGSPAEAPFGDVEAARIIAGTRTLHFVPGMRYSYVNQNFRLLS
                     DILQHRTGRSFAELLRSRIFAPAGMETALLAADTGAMPDGTQGYEGSQAGGFRVAANR
                     ILWTGDAGLGASLDDMIAWERHIDATRDDAGSLYQRLSAPVHFSDGAPAAYGFGLARA
                     MELGRAVTGHGGALRGWRSHRMYVPSERVSVVVMFNHLSDAHQAAVDLLAAVLDETEA
                     PVRQNLAQPAWCGAWRDTETGLAARVEPAAEGQLRLRFGHNPELLSLQQDGSALGSNG
                     LRLRPDSGGLRMLRPQDNQNTVLQAWNGSPTLDVAGRYHCAELDAELTVADAGGALYG
                     AFSGMLGRGRMELLDPVGPDLWTLPCPRALDHTPPGDWTLAFKREAGNVTGVQVGCWL
                     ARRLDYALVS"
     gene            complement(2747..3379)
                     /locus_tag="VFN42_09780"
     CDS             complement(2747..3379)
                     /locus_tag="VFN42_09780"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET8996946.1"
                     /translation="MHARSLFRLAGLLLAALPMLLPRAPAVAHGESPQATILPANIGA
                     LRPPVTPACISSPFGPRILPNHPLAGTFHNGIDLPAPAGAPVHAIAAGRVIRAQRRGP
                     GGLELLVQHNGFVGIYSHLGSVAPAIAEGRLTVRPGEKIGVVGHSGVMYGMHLFFAME
                     REGHFVDPEQYLQLANCHAGMRRRSDMVAADGKIPPTRRIGGHAMAYAAR"
BASE COUNT          549 a         1197 c         1176 g          566 t
ORIGIN      
        1 gcagaagggc gcattgtggt gctcgttcag catgatgccg tcggcaccat actcgtcggc
       61 catgatgtat tcttcgagat actcgttata caggcggctg ccttccaccg ggtcgtaatg
      121 cttgttggag aaggtcagtg cggtggcgcc gtatttcagc ccttccttcg cgtcgtacgc
      181 cgccatcggc tgttcggtga aatacatcag gtgcatggtc cgctcccctt gaaggcagtg
      241 ttggcgcccc gcgtcatggc ccgcgcctgg ggccgccgtg acgcaaggac taatcgacga
      301 atccggtcac cagccgcgcg agttcatcgg gcttttccat atcaacgtag tggccgcacg
      361 agccgatcat ctccagccgt gcgttgcgca gtccgtgctg ccacagcggt gccgcgctgg
      421 ccggcacgtg cctgtcgtca tcaccccaca ccaccagcgc cggggcacgg atgccgccga
      481 tcaggtgcgg caaggtctgg ctgtacatgt acggcttcca cgcggtgcgg aacgacatct
      541 cccggcagat gtcccactgc tcgagctgat cggtcgtgac atcgccatag atgcgcgtga
      601 aggccgcctg gtcgtggaaa ccggcgcgcg gatagtcgat gtacgacacg atcgcctggt
      661 ccatgatgtc gccttccggc ggcttgatgc ccatcggccc ggccagcacc agcttgcgat
      721 aggctgccgg ggccatgctc gccatctcgg cagcgatcca gccgccgaat cccaggccga
      781 ccaacgccac ctcgcgcaat ccaagctccg ccagcagcca ggcatgcatc gcggcaatgt
      841 cgcgtggatg gcgcagccag ttcggccgtt ccgacttgcc ccagccggga tgatgcggca
      901 ccagcacgtc gtagctgccg gcaagtgcgt cgtagaacgc caggcgatcc ggcgtgccga
      961 tatcgtggtg caggatcagc agcgggcggc cactaccggc acgcgagaga tgcatccggg
     1021 taccggcgat ctcgtgcgtc gtgtcgctcc aggccacgct catcggcttc ctccatcact
     1081 tgcttggcaa aaggatactc accggtagct cggcttgtcg aatcggcgac cgagcggcgc
     1141 gatcatggct ttgcagcgca tccgacccgc gcgacacttg cgcgcatgtc catcagcacc
     1201 cgcctcgatc agaccctgtc gcaactacca cagcgctatc ccggcccagg cggcgccgta
     1261 gccgtgctgc gcaacggcga gatcctgctc cgccatgcct ggggctgggg cgacgccgaa
     1321 cggcgcgttg ccttcacccc ccgcacgctg ttccgcatgt gctccatcac caagcagttc
     1381 acctgcgccc tggtgctgga cgccttcccc gacccgtcgg tgctggatgc cgatgtgcgt
     1441 gcgcggctac cgagactgca gcaggatgcg ccgggcgcgc tgcacctgtg ccacaaccag
     1501 tccggcctgc gcgactactg ggccgtcgcc atgctgctcg gctcacccgc cgaggcgccg
     1561 ttcggcgacg tggaagcggc gcggatcatc gccggcacac gcacgctgca ttttgtcccc
     1621 ggcatgcggt actcgtacgt caaccagaac ttccgcctgc tgtcggatat cctgcagcac
     1681 cgcaccggcc gcagcttcgc cgaactgctg cggtcccgca tcttcgcgcc ggcggggatg
     1741 gaaaccgcgc tgctggccgc cgataccggc gcgatgccgg atggcacgca gggctacgaa
     1801 ggcagccagg ctggcggatt ccgcgtggcc gcgaaccgca tcctgtggac cggcgatgcc
     1861 ggcctgggcg ccagcctgga cgacatgatc gcctgggaac gccacatcga tgccacgcgc
     1921 gatgatgcgg gttcgctgta ccagcgcctc tcggcgccgg tgcatttctc cgatggtgcg
     1981 ccggcggcgt acggcttcgg cctggcgcgg gccatggaac tgggccgcgc ggtcaccgga
     2041 catggcggcg ccttgcgcgg ctggcgcagt caccggatgt atgtgccgtc ggaacgcgtc
     2101 tccgtcgtgg tgatgttcaa ccacctgtcg gatgcgcacc aggcggcggt cgatctgctg
     2161 gccgccgtgc tggatgaaac cgaggcgccg gtgcggcaga atttggcgca gccggcatgg
     2221 tgcggcgcct ggcgcgatac ggagaccggc ctggcggcac gcgtggagcc ggcggcggag
     2281 ggccagctgc gcctgcgctt cgggcacaat ccggaactgc tgtcgctgca gcaggacggt
     2341 tcggcactgg gcagtaacgg cctgcggctg cggccggaca gcggcgggct gcggatgctc
     2401 cggccgcagg acaaccagaa cacggtcctg caggcctgga atggttctcc cacgctggac
     2461 gtggccgggc gttatcactg cgcggaactg gatgcggaac tgaccgtggc ggatgccggg
     2521 ggcgcgctgt acggcgcgtt ctccggcatg ctcggccgcg gccgcatgga gctgctcgac
     2581 ccggtcggcc cggatctgtg gaccctgccc tgcccgcgcg cgctggacca cacaccacca
     2641 ggcgattgga ccctggcgtt caagcgtgag gccggcaacg tcacgggcgt ccaggtggga
     2701 tgctggctgg cgcggcggct ggactatgcc ctggtttcgt agcgggtcag cgcgcggcgt
     2761 aggccatcgc atgcccgccg atacgccggg tcggcggtat cttgccgtcc gccgccacca
     2821 tgtcggaccg gcggcgcatc ccggcatggc agttcgccag ttgcaggtac tgctccgggt
     2881 ccacgaaatg cccctcgcgc tccatcgcga agaacaggtg catgccgtac atcacgccgc
     2941 tatggccgac cacgccgatc ttctccccgg ggcgcacggt cagccgcccc tcggcgatgg
     3001 ccggcgcgac cgagccaaga tggctgtaga tgccgacgaa gccattgtgc tgcaccagca
     3061 attccagccc gccggggccg cggcgctgcg cccggatcac ccggccagcg gcgatggcgt
     3121 gcaccggcgc acccgcgggg gccggcaagt caataccatt gtggaacgtg ccggccagtg
     3181 gatgattcgg caggatgcgc ggcccgaaag gggaggaaat gcaggccggc gtcaccggcg
     3241 gccgcagcgc cccgatattc gccggcagga tggtggcctg cggtgattcg ccatgcgcga
     3301 cggccggcgc cctgggcagc agcatcggca gggccgccaa cagcaggccg gcaagtcgga
     3361 agagcgaacg agcgtgcatg aactccaccc cgggcatgga aaagatgcca aagcaatccc
     3421 agcctggctt ggccgagtcc attcagaagc ccgttgagcg gccgctccga cgctgacaat
     3481 gcggtgac
//
LOCUS       DASSZH010000451         2215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1067667, whole genome shotgun sequence.
ACCESSION   DASSZH010000451 DASSZH010000000
VERSION     DASSZH010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067667"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            491..1078
                     /locus_tag="VFN42_09785"
     CDS             491..1078
                     /locus_tag="VFN42_09785"
                     /inference="COORDINATES: protein motif:HMM:NF024744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HET8996947.1"
                     /translation="MAHSRVRYARWFLTAALFAAGVPGGGYAAYCGIIIATGNLHAVE
                     PGRLYRSAQLDKAGLGAVIALHHLHSVLNLRGPNPDSAWYVDELAAARQAGVAHYDVA
                     LSARRAPAPEQIARILDILRTAPKPLLIHCRSGADRTGLVAALYRYAIEHQPAAQAAG
                     ELSLRYGHFPYLTSRTGAMDASFAAFVRAAAEPAH"
     gene            complement(1079..1321)
                     /locus_tag="VFN42_09790"
     CDS             complement(1079..1321)
                     /locus_tag="VFN42_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996948.1"
                     /translation="MAVLEDMFKGASLPGLAVGIGAAVLVPVVAPVVGRMLRPVAKSV
                     FRTGITVWREATSRIAETTGPIVAEARSEMEEARKS"
     gene            1426..1605
                     /locus_tag="VFN42_09795"
     CDS             1426..1605
                     /locus_tag="VFN42_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996949.1"
                     /translation="MRLEESEAIDAVAAAVRRLFGLISERQARDLAAAVLRDLRREGV
                     HLVRGTDDTLPPIRP"
     gene            1756..>2215
                     /locus_tag="VFN42_09800"
     CDS             1756..>2215
                     /locus_tag="VFN42_09800"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET8996950.1"
                     /translation="MDAEPSLAGLRTLLATGSRTAALIAAHDWAATALGPIETWPQSL
                     RTALGLILHSPVPIVMLWGEDGIMLYNDAYAAFAGARHPRLLGSKVREGWPEVADFND
                     NVMKVGLAGGTLAYKDQELTLYRHGRPEQVWLNLDYSPVHDESGRPAGVIA"
BASE COUNT          342 a          761 c          763 g          349 t
ORIGIN      
        1 catcgaagcg ccgcgcggcg gcgatcgcct ccagcgtgtg gcggatctcc gcgtcgctcc
       61 gctcgcccgc gggggcgaag ctgaaattgt tgacgagatc gagcgccagc aggaggagcg
      121 acgcgctgac gatgggcacc agccatatcc agaaaccggc cgcccggagg agaggcttca
      181 ccatgccggc cgcccggacc gcccgcgttg gatggccggg attccgccgt acagcccgat
      241 tgtcgcgtcc tgcgtcatcc catcccgctt gcaagatctg ccagcgattg cacagttccg
      301 gaggcgcttc acccgttcgt ggtatcgaat gacagtgagt taaatcaagc aaccgaggtt
      361 gccaatgtat tttccatatc gcaatgacaa ttcatacgcg cccgacacgc tgccggaaag
      421 ctgacgcatg ggcgggcggg gcttcccctc agggtattcc gaagctttcg tggggggaca
      481 agggggatgg atggcgcatt cacgagtccg ctacgcacgg tggttcctga cggccgcgtt
      541 atttgcggcc ggcgtgcccg gcggcgggta tgctgcgtat tgcggaatca ttattgccac
      601 ggggaatctg cacgccgtgg agccggggcg gctctaccgc tccgcgcaac tggacaaggc
      661 cgggctgggc gcggtgatcg cgctgcatca ccttcactcg gtgctcaacc tgcgcgggcc
      721 gaatccggac agcgcatggt atgtcgatga actcgccgcg gcgcgacagg cgggggtcgc
      781 gcactacgat gtcgccctct cggcacgccg ggcgccggcg ccggagcaga tcgcgcgcat
      841 cctcgacatc ctgcgcacgg cgccgaagcc gctgctgatc cattgccggt caggcgcgga
      901 ccgcaccggg ctggtggcgg ccctctaccg ctacgccatc gaacaccagc cggccgcgca
      961 ggcggccggt gagctgtcac tgcgctacgg gcattttccc tatctcacca gcaggaccgg
     1021 cgcgatggac gcaagtttcg ccgcgttcgt acgggcggcg gccgaacccg cccactagtt
     1081 aggacttccg cgcctcctcc atctccgagc gggcttcggc gacgatcggc ccggtggttt
     1141 ccgcgatccg gctggtcgct tcgcgccaga cggtgatgcc ggtgcggaag accgatttcg
     1201 cgaccggccg cagcatccgc ccgaccaccg gcgcgaccac cggcaccagc accgcggcgc
     1261 cgatgccgac cgcgaggccg ggcaggctcg cccccttgaa catgtcttcc agtacggcca
     1321 tccccgcctc ctgtcacaaa ctcacgacaa cgtggccggc gccgggcggt tgcgtgaaac
     1381 gcccgctgtg cttgcggcgt tcgcgtggct gtgacggaga ggggggtgcg tctggaggag
     1441 tccgaagcga tcgatgcggt tgccgccgcg gtgcggcggc tgttcggtct gatctcggaa
     1501 cggcaggcgc gcgacctggc cgccgcggtg ctgcgtgacc tgcgacgcga gggggtgcat
     1561 ctggtacgcg ggacggacga cacgctgccg ccgatccgcc cctgatccgc gatccggccc
     1621 accccgacgc ggtgagggag cggtgtcgcg cgcctgtccc gacagtcagg atgacggcgc
     1681 cccacgccgg cgtgtagagt ccccggcgaa ggtcacgccg cctttcgtgc cgtgatcacg
     1741 cgaagggacg tgccattgga cgctgaaccg tcgctcgccg gcctgcggac gctgctggcc
     1801 accggcagcc gcaccgccgc gctgatcgcc gctcatgact gggcggcaac ggcgctcggg
     1861 ccgatcgaaa cctggccgca gagccttcgg accgcgctcg ggctgatcct gcactcgccg
     1921 gtgccgatcg tgatgctgtg gggcgaggac ggcatcatgc tctacaacga tgcctacgcc
     1981 gcctttgccg gcgcccggca tccgcgcctg ctcggcagca aggtgcgcga gggctggccg
     2041 gaagtcgccg acttcaacga caacgtgatg aaggtcgggc tggcgggcgg gacgcttgcc
     2101 tacaaggacc aggaactgac gctctatcgc cacgggcggc cggagcaggt ctggctcaac
     2161 ctcgactatt cgcccgtgca tgacgagagc ggccggcccg ccggcgtgat cgcca
//
LOCUS       DASSZH010000452         3718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_521986, whole genome shotgun sequence.
ACCESSION   DASSZH010000452 DASSZH010000000
VERSION     DASSZH010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521986"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            314..1021
                     /locus_tag="VFN42_09805"
     CDS             314..1021
                     /locus_tag="VFN42_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HET8996951.1"
                     /translation="MTLDATTTQKLASVATGTITTVLLKKGLRNVWMRGPKPLRAGQP
                     KLIGRAFTMRFVPAREDLATPESWSSPRSTRAAIEDMPAGCIAVVDAMGVTDAGIFGD
                     ILCARMQQRGVAALVTDGVVRDVKGVLGTNLPVWCQGYAAPASVTGLTFVNWQEPIGC
                     GGVAIFPDDIIMVDDDGAVVIPQKLLADVVDAAYEQERLEEWIMIQVNAGAALPGLYP
                     ANAENKARYEEWRRSQG"
     gene            1163..1762
                     /locus_tag="VFN42_09810"
     CDS             1163..1762
                     /locus_tag="VFN42_09810"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET8996952.1"
                     /translation="MFAVLFAVEPHPERWDDYLRYAAALRPELERIDGFLDNRRFRSR
                     RHDGRLLSLSLWRDEKSLVRWRAHGGHHAIQAKGRAAIFRDYHLRVGEVTRQDARALP
                     QSRLDVTETGTAQAVSVLEMPAAEVPPAAAGLVDWDMFDGITDPQSRLLLLSWRDEPA
                     MLASAAPTASRRLDVRIVRDYGLHDRAEAPQFHAQVDRR"
     gene            complement(1809..2552)
                     /locus_tag="VFN42_09815"
     CDS             complement(1809..2552)
                     /locus_tag="VFN42_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET8996953.1"
                     /translation="MDSLETNKVIAAVLVAGIAFFVAGTIADQLIVQPELKHTVLKIE
                     GAPATVTSSEPKPVALPPLAPLLAKADVKKGEDFAHKVCAACHTFNQGGKPGVGPNLY
                     GIVGDPHAHEKGFDYSDAIKSKKGPWTYAELNEWLHKPSSYAPGTRMPFAGIPQAQLR
                     ADVIAFLKSISPKAPPFPKPEAAAPAKPASTTPAAANAVGGKAAGTNPAPAKPAPAAS
                     APAAPAPAASAPAAPAPAAPAPAAPPAKQ"
     gene            2691..3440
                     /locus_tag="VFN42_09820"
     CDS             2691..3440
                     /locus_tag="VFN42_09820"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010513425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HET8996954.1"
                     /translation="MNPIVVIPSRLASTRLPGKPLADIHGKPMIVHVLERALEAGIGP
                     AAVACGDSAIAEVVRAHGGTAVMTDPSLPSGSDRVHAALAVLDPQGRYDVVVNLQGDL
                     PTIPAVQLRAVVTPLADPAVDIATLVAPIESEQEANLPSVVKAACVFARGRAVSNVLY
                     FSRAPIPAGDGPRWHHIGVYAYRRAALARFVALPESPLEGREHLEQLRALEAGMRIAA
                     ARVDFAPFGVDTPDDLERARRALQPQGAKTL"
     gene            3437..>3718
                     /locus_tag="VFN42_09825"
     CDS             3437..>3718
                     /locus_tag="VFN42_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HET8996955.1"
                     /translation="MSGTIAFQGMPGAYSDLACRVARPNMTTLPCENFETAMEAVRDG
                     SADLAMLPCENSLAGRVPDIHSLLPESGLHVIGEHFQRVEHCLLAPRGAT"
BASE COUNT          546 a         1293 c         1297 g          582 t
ORIGIN      
        1 tgtcctgggt gtgaaatcct acgggcctgc caacccgctc agtcgctgcg ggcagggccg
       61 ctcgagcgga gatcggcgga gccccttggg tccgccgctt atggtcttgc cacgccggga
      121 acaccaagcg tcaccacgac attcggaatg tcgcgcgccg gctcccgaac cgcacaggag
      181 ccccacccgg ccacgccgcc tttcgccagc cacggcaccc gccgccggcc ccgccactct
      241 ggcctttgcc gcggcgccga cgcagtatcc cgcccttccc ttacctgccg tcttcagccg
      301 tacggagcct gcgatgacgc ttgacgccac cacgacccag aaactcgcct cggtcgccac
      361 cgggactatc accaccgtgc tgctgaagaa ggggctgcgg aatgtctgga tgcgcgggcc
      421 gaagccgctg cgcgccggcc agccgaagct gatcggccga gccttcacca tgcgcttcgt
      481 gccggcgcgc gaggacctgg cgacgccgga atcctggtcg tcgccgcgct ccacccgcgc
      541 cgcgatcgag gacatgccgg caggctgcat cgccgtggtg gatgccatgg gcgtcaccga
      601 tgccggcatc ttcggcgaca tcctgtgcgc ccgcatgcag cagcgcggcg tggcagccct
      661 ggtcaccgac ggcgtggtgc gcgacgtgaa gggcgtgctg ggcaccaacc ttccggtgtg
      721 gtgccagggc tacgcggcac cggcctcggt caccggcctg accttcgtca actggcagga
      781 gccgatcggc tgcggcggcg tcgcgatctt cccggacgac atcatcatgg tggacgatga
      841 cggtgcggtg gtgatcccgc agaagctgct cgccgacgtg gtcgacgccg cgtacgagca
      901 ggagcggctg gaagaatgga tcatgatcca ggtgaatgcc ggcgccgcgc tgcccggcct
      961 gtatccggcc aatgccgaga acaaggcccg ctacgaggaa tggcggcgca gccagggctg
     1021 atccgcggac agcgccaggc gcccgggcgc gcgcaccgga cgcctggcat cgttcgaccg
     1081 cggccgaagc gagccgcggt gcaagcgccg ccgccagggg ctagcctcga cagcggacaa
     1141 cgttgcagga gggtctgcgg tcatgtttgc agtgctgttc gcggtggaac cgcaccccga
     1201 gcgctgggac gactaccttc gctatgcggc tgcgctgcgc cccgaactgg aacgcatcga
     1261 cggcttcctg gacaaccggc gcttccgcag ccgccggcat gacggacgcc tgttgtcgtt
     1321 gtccctgtgg cgcgacgaga aatccctggt ccgctggcgc gcgcatggtg gccaccatgc
     1381 gatccaggcg aaggggcgcg cggcgatctt ccgcgactac cacctgcggg tcggagaggt
     1441 gacgcgccag gacgcccgcg ccctgccgca gtcacggctg gacgtgacgg agaccggtac
     1501 ggcccaggcg gtttccgtgc tggagatgcc tgcggcggag gtgccgccgg cggcagcggg
     1561 cctggtggac tgggatatgt tcgacggcat caccgatccg cagagccggt tgctgctgct
     1621 gtcctggcgc gacgagccgg cgatgctggc cagcgccgcg cccaccgcat cgcgccggct
     1681 ggatgttcgg atcgtacgcg actacggcct gcacgatcgg gcagaggcgc cgcaattcca
     1741 cgcccaggtc gaccgccgat aagccgcagc ctggcggagc ctgccgctcc gccaatccgc
     1801 cgcggcggtt attgcttcgc gggcggcgcg gcgggggccg gcgcggcggg ggccggcgca
     1861 gcaggcgccg aagcggctgg agccggcgcg gcaggcgccg aagcggcggg cgccggcttg
     1921 gccggggcgg ggtttgtgcc ggcggccttg ccgcccaccg cgttggccgc ggccggagtc
     1981 gtgctggccg gcttggccgg tgccgcagcc tcaggcttcg ggaagggcgg tgccttgggc
     2041 gaaatgctct tcaggaaggc gatcacgtcg gcgcgcagtt gcgcctgggg gatgccggca
     2101 aacggcatgc gcgtgcccgg cgcgtaggag ctgggcttgt gcagccactc gttcagctcc
     2161 gcataggtcc aggggccctt cttcgacttg atcgcgtcgg agtagtcgaa tcccttctcg
     2221 tgggcatgcg gatcgccgac gatgccgtag aggttgggcc cgacgcccgg cttgccgccc
     2281 tggttgaagg tatggcacgc ggcgcagacc ttgtgggcga agtcctcgcc cttcttcacg
     2341 tcggccttgg ccagcagcgg cgccagggga ggcaatgcca ccggcttcgg ctcgctgctg
     2401 gtcaccgtgg caggcgcgcc ctcgatcttc agcaccgtat gcttcagctc gggctgcacg
     2461 atcagttggt cggcgatcgt tcccgcaaca aaaaaggcaa tgcccgcaac caagacggcg
     2521 gcaatgacct tgttggtctc caagctatcc atgaaccgag acttccccca tcgcttcagc
     2581 gttcagccgg acccgacccg ccgttgcatg gggccgttcg cgtccatatt gtgcatgcgc
     2641 accataggca aggccgtttt ctgcttcaac ccaccaggct gaccgctccc ttgaatccga
     2701 tcgtcgtcat tccttcgcgc ctggcatcga cccgtttgcc cggcaaaccg ctggccgaca
     2761 tccacggcaa gccgatgatc gtccacgtgc tggagcgtgc gctggaagcc gggatcggac
     2821 ccgccgcggt ggcctgcggc gattcggcga ttgccgaggt ggtgcgggcg catggcggca
     2881 ccgcggtgat gaccgacccg tcgctgcctt ccggctccga tcgcgtgcat gcggcactgg
     2941 cggtactgga cccgcaaggc cgctacgacg tggtggtgaa cctgcaaggg gacctgccga
     3001 cgatacccgc agtgcaattg cgcgccgtcg tcacgccctt ggccgacccg gcggtcgata
     3061 tcgccaccct ggtggcgccc atcgaatcgg agcaggaggc gaaccttccc tcggtggtga
     3121 aggccgcctg cgtgttcgcg cgcggccgcg cggtgtcgaa cgtgctgtac ttctcccgcg
     3181 cgccgattcc cgccggagac ggcccgcgct ggcaccatat cggcgtgtat gcgtaccgca
     3241 gagcagcgct ggcccgattc gtggcgctgc cggaaagccc gctggaaggg cgcgagcacc
     3301 tggagcaact gcgcgcgctg gaggccggca tgcgcattgc cgccgcgcgg gtggacttcg
     3361 caccctttgg ggtggacaca ccagacgatc tggagcgcgc gcgccgcgcg ctgcagccac
     3421 aaggagccaa gacgctatga gcggcacgat cgccttccag ggcatgccgg gtgcctattc
     3481 cgacctggcg tgccgtgtcg ctcggccgaa catgacgacc ctgccttgcg agaacttcga
     3541 gaccgcgatg gaagcggtgc gcgacgggtc ggccgacctc gccatgctgc cctgcgagaa
     3601 cagcctcgcc ggccgcgtgc cggacatcca ttcgctgctg cccgaatcgg gactccacgt
     3661 catcggcgag catttccagc gggtcgagca ttgcctgctg gccccgcgcg gcgcgacg
//
LOCUS       DASSZH010000453         2503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1306438, whole genome shotgun sequence.
ACCESSION   DASSZH010000453 DASSZH010000000
VERSION     DASSZH010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306438"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1995
                     /locus_tag="VFN42_09830"
     CDS             <1..1995
                     /locus_tag="VFN42_09830"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HET8996956.1"
                     /translation="PRAVAVVGASDDSTKHGYIVLNNLRDTGFPGGVYGISRRLQAVN
                     GIPCYPDLTSVPEQIDVAFLAIPAEAAVQAVRDAARAGLKAVIVGSAGYAESLDAGGA
                     ERQAELARIAHEEGIRIVGPNCNGIYNAHHPLSIGFNTSHAKRQAPGGISIFSHSGAL
                     FDAMAGRLRMLGAGLSLFASAGNEADLSVLDYMEYGIGHAPTRVIALLIDSLDDGTRF
                     RRLALAAHAAGKPVVVLKIGTSDAGAQAAVAHSSRIAGDTAAYAALLESCGVSRVATL
                     EGLMAASALLDRYGRQHGALGAMSTSGAGASLIADRAAALSIPLATFTEHTHRQIDAQ
                     KVFSRIGNPLDLGIFGGMRRSASVPTLLLCDPGVGAVLALVHSMNPWQGDPYRAALAA
                     ARETSGKPLLVVTPGGLPEHERAWYETHGMPVFTETDIVLEAAGALLTPPPDALPETP
                     PAIAPSLPARTLTEPESLRLLGGFGVPVVTTVECDSLDQARDAAAQVGWPVVLKGVAE
                     GAAHKSELGLVRTNLHDAAALEAAYAALNCPHVIVQPMVSGELEAIAGISRSPGVGLV
                     LLAGLGGIYAEALRDVCVWPVPVSRAAVERSLADSSLGRVLRSPRWRHPDATRAFVDL
                     LLRLQSMAQTLHDRLEAVDINPVMLGANGAVAVDALVVPR"
     gene            2135..>2503
                     /locus_tag="VFN42_09835"
     CDS             2135..>2503
                     /locus_tag="VFN42_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose 1,5-bisphosphate carboxylase"
                     /protein_id="HET8996957.1"
                     /translation="MRLSVTYHVRSDAASIDARAQGIAVEQSVEMPLAAIDDPEIFSD
                     IVGAVEDIADLHDGRFAVRIGLALSTIGSDAGQLLNMLFGNTSLHEDVVLHDIALPPA
                     LIAGFPGPRTGIAGLRARVRA"
BASE COUNT          377 a          848 c          858 g          420 t
ORIGIN      
        1 ccgcgcgccg tggcggtggt cggcgcttcc gacgacagca ccaagcacgg ctacatcgtg
       61 ctgaacaacc tgcgcgacac gggattcccc ggcggcgtgt acggcatcag ccgccggttg
      121 caggcggtga atggcattcc gtgctatccc gatctgacct cggttccgga acagatcgac
      181 gtggcgttcc tggcgatccc ggcggaagcc gcagtgcagg ccgtacgtga cgcggcacgc
      241 gccgggctga aggccgtcat cgtcggttcg gccggttatg cggagagcct ggacgccggc
      301 ggcgccgaac gccaggccga actggcccgc atcgcccacg aggaaggcat ccgcatcgtc
      361 ggcccgaact gcaacggcat ctacaacgcg caccatccgc tctcgatcgg cttcaacacc
      421 tcgcacgcga agcgacaggc ccctggcggc atttcaatct tctcccattc cggtgcgctg
      481 ttcgatgcca tggccgggcg gctgcgcatg ctgggcgccg gattatcgct gttcgcctcg
      541 gccggaaatg aagccgacct gtccgtgttg gactacatgg aatacgggat cggacatgcg
      601 ccgacgcgcg tcatcgcgct gctgatcgac agcctggatg acggcacgcg cttccgccgc
      661 ctggcgctgg ccgcgcatgc ggccggcaaa cctgtcgtcg tcctgaagat cgggacatcg
      721 gatgccggtg cacaggccgc ggtggcacat tcgtcgcgca tcgccggtga caccgcggcg
      781 tacgccgcgc tgctggagtc gtgcggcgta tcacgggtcg cgacactgga agggctgatg
      841 gccgccagcg cgctgctcga ccgctatggg cggcagcacg gtgcgctggg tgcgatgtcc
      901 acctccggcg ccggcgcatc gctcatcgcg gatcgcgccg ctgcgctgtc cattcccttg
      961 gcgacattca ccgaacacac ccatcggcag atcgatgcgc agaaagtgtt ctcccgcatc
     1021 ggcaatccgc tggatctcgg catcttcggc ggcatgcggc gttcagcttc ggtgccgacg
     1081 ctgctgctgt gcgatccggg cgtcggcgcg gtgctggcct tggtgcactc gatgaatccc
     1141 tggcaaggcg atccgtatcg cgccgcactg gccgcggcac gcgaaacctc cggcaagccg
     1201 ctgctggtcg tcacgcccgg cggcctgccg gagcatgaac gcgcgtggta cgaaacgcac
     1261 ggcatgccgg tgttcaccga gaccgatatc gtgctcgaag ccgccggtgc gctgctgact
     1321 ccgccgccgg acgctctacc cgagaccccg cccgccatcg cgcccagctt gccggcgcgc
     1381 accctgaccg agccggaaag cctgcgcctg ttgggcggct tcggcgtgcc ggtggtgacc
     1441 acggtggaat gtgactctct ggaccaggcc cgtgatgcgg cggcgcaggt cggttggccg
     1501 gtggtgctga aaggcgtcgc ggaaggcgcg gcgcacaaaa gcgagcttgg cctggtgcgc
     1561 accaacctgc acgacgctgc ggcgctggag gcagcctatg cggcgctgaa ttgcccgcac
     1621 gtcattgtgc agccgatggt aagcggggaa ttggaggcca tcgccggcat cagccgttcg
     1681 cccggcgtcg gcctggtgtt gctggcgggg ctcggaggca tctacgccga ggcattgcgc
     1741 gacgtgtgcg tctggccggt gccggtgtcg cgcgccgcgg ttgaacgcag cctggccgac
     1801 agcagtctcg gccgtgttct gcgcagcccg cgctggcggc atccggacgc aacccgtgcg
     1861 tttgtcgatc tgctgctgcg gctgcagagc atggcgcaga cgctgcatga ccggctggag
     1921 gcggtggaca tcaacccggt gatgctcggc gcaaacggtg cggtcgcggt ggatgcgctg
     1981 gtggtgccac gatgagcgac gattcccgtg gtaccgggcc aaggcccgca attcatgcac
     2041 tgctgtgggg gactcccttg aagagccggg gtgcagcact gagtttcccc caacaattcg
     2101 tgggtggcgg gccttcgccc gccacgacgt gaccatgcgc ctgtccgtta cctatcacgt
     2161 ccggagtgat gcggcgtcga tcgacgcgcg cgcgcagggc atcgcggtcg agcagagcgt
     2221 ggaaatgccg ctcgccgcga tcgatgatcc ggaaatcttc agcgacatcg tcggagcggt
     2281 cgaagacatc gccgatctgc acgacggacg cttcgccgtg cgcatcggac tcgccctgtc
     2341 caccatcggc agcgatgccg gccagctgct caacatgctg ttcggcaaca cctcgctgca
     2401 tgaggacgtc gtgctgcacg acatcgcgct tccgccggcg ctgatcgccg gctttcctgg
     2461 cccgcgcacc ggcatcgccg gcctgcgcgc cagagtccgt gcc
//
LOCUS       DASSZH010000454         3143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_880089, whole genome shotgun sequence.
ACCESSION   DASSZH010000454 DASSZH010000000
VERSION     DASSZH010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880089"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VFN42_09840"
     CDS             complement(<1..698)
                     /locus_tag="VFN42_09840"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET8996958.1"
                     /translation="MEGGYRISGKWRFASGSHHASWLGAHMKVAGAAKERTMLFPKSS
                     VQMNDIWHTLGLRGTGSDEYVVDNLFVPQRFTMARDTAAERRESGPLYRFTSNQLYSC
                     GFAGVGLGIARATMRDFLDLPATKVSRGATRPLRENNVVQSQFAQCEAKWRSARAFLH
                     TTWDQAWLNVEAQGEQTQEDRTLIRLASTWAIQQSREIVSTLYHAAGSIAVFEEQPFE
                     QRLRDIHTVAQQSQG"
     gene            complement(1157..2068)
                     /locus_tag="VFN42_09845"
     CDS             complement(1157..2068)
                     /locus_tag="VFN42_09845"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8996959.1"
                     /translation="MDVRFDDRVAIVTGAGAGIGRCHALLLASRGAKVVVNDPGGAVD
                     GTGTASSVADKVVAEIKAAGGEAVANYDSVAEEQAAQRIIDTAMKTWGRLDILVNNAG
                     ILRDKSFNNMDMADYAFVNQVHHFGTVYCTKAAWPIMRAQKYGRIVVTTSSSGTVGNF
                     GQANYGAAKMAVVGLINVLRHEGGKYGIRINAISPSALTRMTESLLPPDVGPWMKPEL
                     VSPAVAWLCSEECDQNGEIIAATAGGYARVQYFVTDGAQFDPAEPVTIEMVRDSIGRI
                     RDLSTAKPYTGLMGNVEQKLRDMGRIK"
     gene            complement(2076..2963)
                     /locus_tag="VFN42_09850"
     CDS             complement(2076..2963)
                     /locus_tag="VFN42_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8996960.1"
                     /translation="MSDPEIARLREMIASRPRATELAQRRRDIDARGKLFGIASDVTV
                     EKISANGVPAEWTATPDADPASAVLYVHGGGYVIGSLDSHRHLVSELGRAAGARVLAL
                     DYRLAPEHPFPAAVEDTVAGYRHLLSSGIAPQRIAIAGDSAGGGLVVGAMLALREAGL
                     PLPGCGWCISPWVDMEATGASFTELAAIDPIVQKAGIAEMAGYYLNGADPRSPHAAPI
                     YGDLRGLPPLLIQVGAAETLLDDALTLARAAGAADVFVELQVWPEMIHVWHALHPMLS
                     AGRRAIEAGGAFVRATTRG"
BASE COUNT          490 a         1103 c         1023 g          527 t
ORIGIN      
        1 ccctgcgact gctgcgccac cgtgtggatg tcacgcaacc gttgttcgaa gggctgttcc
       61 tcaaataccg cgatcgaacc cgcggcatgg tagagcgtgc tgacgatctc ccgcgattgc
      121 tggatcgccc aggtggatgc caggcggatc agcgtgcggt cttcctgagt ctgctcgccc
      181 tgggcttcca cgttaagcca ggcctggtcc caggtggtgt gcaggaacgc ccgggcggat
      241 cgccacttcg cctcgcactg cgcgaattgc gactgcacca cgttgttttc ccgcagcggg
      301 cgggtggcgc cgcgggacac cttggtggca ggcaggtcca ggaagtcccg catcgtggcc
      361 cgcgcgatgc ccagcccgac cccggcgaaa ccgcaggaat acaactggtt gctggtgaac
      421 cggtacaagg ggccggattc ccgccgctcc gccgcggtgt cgcgcgccat ggtgaagcgc
      481 tgcggcacga acaggttgtc caccacgtac tcgtcgctgc cggtgccgcg cagcccgagc
      541 gtgtgccaga tgtcgttcat ctgcacgctg gatttcggga acagcatggt gcgttccttc
      601 gccgcacccg ccaccttcat gtgcgcgccg agccaggagg catggtggct gccgctggcg
      661 aaacgccact tgccggagat gcggtagccg ccttccaccg gcttggcctc gtggtgcgcc
      721 gggcggcccc caggccagga tgccggtggg cggaccgaag atctccgtgg cagtttcccg
      781 gtccagatag gcggccgaca tggcgcagcc attgttctgg cagacgcacc aggcagtgga
      841 ggcgtcggcc gaggccagtg cttcggtgac ctgcgcatag gccaccgggt gcaattcggc
      901 gccgccgagg aatttcggtt gcagcatgcg gaagaagccc tgggagatca gggcggagac
      961 cagccgctcg ggcaattcgc gcccggtgtc gatttcgtcc gaggcctcgg caagcaaagg
     1021 cgcgatctcc cgcgccttcc ggatcatgtc gtgctgttgc gtcacttgtg tccctccgcc
     1081 tctcgcaagc ctgccagccg cagcggacgc cgtgaagtcc ccggggctgg ccgccggaag
     1141 gatggctgat ccggtcctac ttgatccgtc ccatgtcgcg cagcttctgc tccacattgc
     1201 ccatcaggcc agtatacggc ttggcggtgg acaggtcgcg aatgcggccg atcgagtcgc
     1261 gcaccatctc gatcgtcacc ggctccgccg gatcgaactg cgcgccgtcg gtgacgaaat
     1321 actgcacccg cgcatagccg cccgcggtcg cggcgatgat ctcgccgttc tggtcgcatt
     1381 cctcgctgca cagccaggcg accgccggcg agaccagctc cggcttcatc caaggcccga
     1441 catccggcgg cagcagcgac tcggtcatgc gcgtcagcgc cgagggcgaa atcgcattga
     1501 tgcggatgcc gtatttcccg ccttcatgcc gcagcacgtt gatcagcccg accacggcca
     1561 tcttcgccgc gccgtaattc gcctggccga aattgcccac cgtgccggaa ctggaggtgg
     1621 tgaccacgat gcgcccgtat ttctgcgcgc gcatgatcgg ccacgccgcc ttggtgcagt
     1681 acaccgtgcc gaaatgatgc acctgattga cgaacgcgta gtccgccatg tccatgttgt
     1741 tgaagctctt gtcgcgcagg atgccggcgt tgttcaccag gatgtccagc cggccccagg
     1801 tcttcatcgc ggtgtcgatg atgcgctgcg ccgcctgctc ctcggcgacg gagtcataat
     1861 tcgccacggc ttcgccgccg gccgccttga tctctgccac caccttgtcg gccacgctgc
     1921 tggcggtgcc ggtgccatcc accgcgccgc ccggatcgtt caccaccacc ttggcgccgc
     1981 gcgacgccag cagcagcgcg tggcagcggc caatgccggc accggcgccg gtgacgatgg
     2041 cgacgcgatc atcgaaacga acgtccattt cccaattatc cccttgtggt ggcacgcacg
     2101 aaggcgccgc ctgcctcgat cgcccggcgg ccggcgctca gcatcggatg caacgcgtgc
     2161 cagacatgga tcatctccgg ccacacctgc agctccacga agacgtccgc cgcgcccgcg
     2221 gcgcgcgcca gagtcagcgc atcgtccagc agcgtttcgg ccgcgccgac ctggatcagc
     2281 agcggcggca gtccgcgcag gtcgccgtag atcggcgcgg cgtgcggcga gcgtggatcg
     2341 gcaccgttca gatagtaccc ggccatttcc gcaatgccgg ccttctgcac gatcggatcg
     2401 atcgcggcga gctcggtgaa ggatgcacca gtggcctcca tgtccaccca gggggagata
     2461 caccagccgc agcccggcaa gggcagcccc gcctcgcgca gcgccagcat ggcgcccacc
     2521 accaatccgc cgccagcact gtcaccggcg atggcgatgc gttgcggcgc gatgccggac
     2581 gacagcaggt ggcggtagcc ggcgacggtg tcctcgactg ccgccggaaa cgggtgttcc
     2641 ggggccagcc ggtaatccag cgccagcacg cgcgcaccgg ccgccctgcc cagttcggac
     2701 acgagatggc ggtgactgtc gagtgagccg atcacatagc cgccgccatg cacatacagc
     2761 acggcgcttg ccggatcggc gtcgggagtc gcggtccatt ccgccggcac gccgttggcg
     2821 ctgatcttct ccaccgtcac atcggacgcg atgccgaaca gcttgccgcg cgcatcgatg
     2881 tcgcggcggc gctgggcgag ctcggtggca cgcggccggc tggcgatcat ctcacgcagc
     2941 cgggcaattt ccggatcgct cacgcttccc tcctgtcctt ctccccggtc attccaaccg
     3001 gaaagcggcg ggctggcaaa tgcacgcgcg gcgtgacacc ggatcaggag gctggcggtt
     3061 ccgccatgct gtgggtcgtg ctccgcgcac gcaagctggc caaggctcgg ctcagcggtg
     3121 ggcccgcgcc ttcacgcgcg aca
//
LOCUS       DASSZH010000455         6086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1630173, whole genome shotgun sequence.
ACCESSION   DASSZH010000455 DASSZH010000000
VERSION     DASSZH010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6086
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630173"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            823..1500
                     /locus_tag="VFN42_09855"
     CDS             823..1500
                     /locus_tag="VFN42_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996961.1"
                     /translation="MLLILVHALLVYARHWARLFGQAAERGIALLAHCCGSAQASFVL
                     SHFTRGILRAIALQRVLSARAVGEPAGNAAGAASGMPRKRTPPAGRHSVSGASHDQLT
                     ALPTLRQLEAAISRRPVEDVIDDISRDLGAAFSLCDIRFGNALRDMAAAYRDTLRSFL
                     HECRQREAAVADAPRRILPFALPPQKPGAIRYIVGCLGVQQPVCGFPFTAAPFGAAIA
                     GAQPPGT"
     gene            complement(1791..2531)
                     /gene="eutC"
                     /locus_tag="VFN42_09860"
     CDS             complement(1791..2531)
                     /gene="eutC"
                     /locus_tag="VFN42_09860"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HET8996962.1"
                     /translation="MTSRSDVWASLRSATPARIGLGRRGDAPPLEAMLAFQLAHARAR
                     DAVHTALDVPALGAALGGAVVVRSAAPDRDVYLRRPDLGRRLHPSSRAVLTGVARPED
                     GWDAVFVLADGLSAAGVQRSAVPLLEACRTRLPGWSLAPVVIATQARVALGDAIGEVL
                     GARLCVMLIGERPGLSVANSLGVYLTWQPCIGRRDAERNCVSNIHPDGLSCDAAAEQV
                     TWLMTEARRRGLTGVALKRDGVAAALPG"
     gene            complement(2515..3912)
                     /locus_tag="VFN42_09865"
     CDS             complement(2515..3912)
                     /locus_tag="VFN42_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HET8996963.1"
                     /translation="MPSYASSFQGERHRFSDLRDVLAKASPLRSGDQLAGLAADSDAQ
                     RVAARALLADLPLRIFLDEPLVPYETDDVTRLILDTHDAAGFAALSHMTVGDFRDWLL
                     SYEADGTQLARLAPAITPEMAAAVSKLMRNQDLISVARRCRVTTAFRNTQGLPGRLGT
                     RLQPNHPTDDLRGVAAATLDGLLYGTGDAVIGVNPAGDDIPTCMLLLEMLDALRQRHD
                     VPTQSCVLTHVTNTLEIIRRGAPVDLVFQSIAGSQAANAGFGVTLDLLREAQQAALSL
                     QRGSIGNNVMYFETGQGSALSAEAHHGVDQQTMEARAYAVARAFSPLLVNTVVGFIGP
                     EYLYDGKQIVRAALEDHFCGKLLGLPMGCDACYTNHAEADQDDMDTLITLLTVAGVTF
                     LIVVPGADDVMLNYQSAAFHDMLYLRSVLQVRAAPEFEAWLESMEMTDRAGRIRPLPP
                     TVVPLLLADTHDQPV"
     gene            complement(4313..4657)
                     /locus_tag="VFN42_09870"
     CDS             complement(4313..4657)
                     /locus_tag="VFN42_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996964.1"
                     /translation="MHTNDCEPSLCLAALRSLTALSAFLDVSSLNLAAPQSAAIFLSG
                     HHRARIRLAPGRRRTRGGAERDNRQGVQRSAIPVLLPERVVVMVMMMMMMMADDGMVM
                     MMVVVVMRPRGA"
     gene            4872..5168
                     /gene="rplU"
                     /locus_tag="VFN42_09875"
                     /pseudo
     CDS             4872..5168
                     /gene="rplU"
                     /locus_tag="VFN42_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
     gene            5328..5597
                     /gene="rpmA"
                     /locus_tag="VFN42_09880"
     CDS             5328..5597
                     /gene="rpmA"
                     /locus_tag="VFN42_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008851550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET8996965.1"
                     /translation="MAHKKAGGSSRNGRDTEGRRLGIKKFGGESVIAGNILVRQRGTK
                     WKPGQNVGLGRDHTIFALVDGKVEFSRKADDRVHVSVRPLPVAAE"
     gene            5674..>6086
                     /gene="obgE"
                     /locus_tag="VFN42_09885"
                     /gene_synonym="cgtA"
                     /gene_synonym="obg"
                     /gene_synonym="yhbZ"
     CDS             5674..>6086
                     /gene="obgE"
                     /locus_tag="VFN42_09885"
                     /gene_synonym="cgtA"
                     /gene_synonym="obg"
                     /gene_synonym="yhbZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422157.1"
                     /note="ObgE; essential GTPase; exhibits high exchange rate
                     for GTP/GDP; associates with 50S ribosomal subunit;
                     involved in regulation of chromosomal replication; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET8996966.1"
                     /translation="MKFLDQAKIYLRSGDGGDGVVAFRREKCLEFGGPDGGNGGRGGD
                     VVFEAAANLNTLIDFRYTQHFRAPKGGNGAGSNRTGAGARDVVIAVPIGTVILDEDRE
                     TVLADLDAPGKRIVLLRGGDGGHGNAHFKTSTNRAP"
BASE COUNT          991 a         2161 c         2009 g          925 t
ORIGIN      
        1 tggtgtccga cgccgccgcc gagatggccg aaccgggctt ccatcccagt ccggcccagg
       61 tcgccagtgc gatcctgtcc gtcgcggtgc accgtctcgg tccggacctc aagcacgacg
      121 ccaggcaagc actcaaggcg gccggcaggt cgacatagcg gttgaccggc aacgcttcag
      181 gcagcgcaac gcagtgcaac cgcagaagtc gccggtttga ttgcatttcg cttgctctat
      241 cggttgccgt gtatcggaat agccagggta gaccagtcgg ccatgcagcc cggtcaccac
      301 gactcgcagg caaccccgtc gctgtcaccg tccagccagg gcgcatagta gggtgagccc
      361 ctcggaatgt tcaccagccc catggcgcgc gcctgggtgc agtttctcgg agcgctgacg
      421 ccgacaggtg tgatcaccat cgggagagat ggcatcgatc ggaagtggtc caccaccgcc
      481 accgcaagaa tcagcaacaa ggccagcatc ctcataccgg tccgcagcct gcgatcgtgc
      541 ctgctcaggg gacgccaatg cttcgtatag cggttacggt agcgcatcgc ccgagcctgc
      601 gggagcagac gttacggcaa agtttcgtca tcccaaccgg gccggcgccg ctccccgtcc
      661 tggggtctgc gcgcgggtct cacaaaaaaa gcttgacata actaacgacg tcagctatat
      721 tcgccggcta tgaacccagc acacacctcc ttccccagcg tgcctctccc gtcccctcca
      781 ctccagaacc ggtttgcccg gccgctgccg ccggaagccg aggtgttgct catcctggtc
      841 cacgccctgc ttgtctatgc gcgccattgg gcccgcttgt tcgggcaggc agcagaacgc
      901 ggcatcgcgc tgctcgcgca ttgctgcggc tcggcgcagg ccagcttcgt tctctcccat
      961 ttcacccgcg gcatcctgcg cgccatcgcg ctgcagcgcg tgctgtcggc gcgtgccgtt
     1021 ggcgagcctg ccgggaacgc cgccggtgca gcctccggaa tgccgcgcaa gcgcacaccg
     1081 ccggcggggc ggcattccgt gtccggtgct tcccacgacc aactcaccgc gctgcccacg
     1141 ctgcggcagc ttgaggcggc catcagccgc cggccggtcg aggacgtcat cgatgacatc
     1201 agccgcgatc tcggcgccgc gttcagcctg tgcgatatcc gcttcggcaa cgcgctgcgc
     1261 gacatggctg cggcatatcg cgacacgctc cgcagcttct tgcatgaatg ccgccagcgc
     1321 gaagcagccg ttgccgacgc gccgcggcgg attttaccct ttgccttgcc accacagaaa
     1381 cccggtgcca ttcgctacat cgtgggctgc ctaggcgtgc agcagcccgt ctgtggtttt
     1441 cccttcaccg ccgcgccgtt cggcgcggcc attgcaggag cgcagccgcc gggaacatga
     1501 acgacacgcg cggcggcaga cgctgcgcac gacaagcaag atctgacgca aaaaacgcaa
     1561 cgaaccgcaa agaacgcgaa tgcagcggag gtttatccgg ggacccgggt acgccacggc
     1621 catttgcgtt cgttgcgttt ttgcgttcaa tctttgtcac gcacgtccgt gccagccacc
     1681 tggcttggtc cgagtgccgc cgtctatcgc ggaacacgcg gcaaagacgt gggtggccgg
     1741 gccaggcccg gccatgacat gcggggcgca acacgagggc gcatgatccg tcagcccggc
     1801 agcgccgccg ccaccccgtc gcgcttcagc gctacgccgg tcagcccccg ccggcgcgcc
     1861 tccgtcatca gccacgtcac ctgctccgcc gccgcatcgc aggacagccc gtccgggtgg
     1921 atgttggaca cgcagttgcg ctccgcatcg cgccggccga tgcatggctg ccaagtgaga
     1981 tagacaccca ggctgttggc gacgctcaac cccggccgct cgccgatcag catcacgcac
     2041 agccgagcac ccaacacttc gccgatcgcg tcgcccagcg cgacccgcgc ctgggtggcg
     2101 atcaccaccg gcgccagcga ccagcccggc aagcgcgtgc ggcacgcctc cagcagcggc
     2161 acggcggagc gctgcacgcc ggccgccgac agcccgtcgg caagcacgaa caccgcatcc
     2221 cagccgtcct ccggccgagc gacccccgtg agcactgctc gcgacgacgg gtggagccgg
     2281 cggccaaggt ccggccggcg cagatacacg tcgcggtccg gcgcggcgct gcgcaccacc
     2341 accgcgccgc ccaacgcagc tcccagcgcc ggaacgtcca gcgccgtgtg caccgcatcc
     2401 cgcgcccgcg catgcgcaag ctggaacgcc agcatcgcct ccagcggcgg cgcgtcgccg
     2461 cgccgtccca ggccgatccg cgccggggtg gcggaccgca gcgacgccca gacgtcagac
     2521 cggctggtca tgcgtgtccg ccagcagcag gggaaccacc gttggtggca gcgggcggat
     2581 gcgccccgcg cggtccgtca tctccatgct ttccagccat gcctcgaact ccggcgccgc
     2641 ccgcacctgc aacaccgagc gcaggtacag catgtcgtgg aacgccgcgc tctggtagtt
     2701 cagcatcaca tcgtcggcgc cgggcacgac gatcaggaag gtcacgccgg ccacggtaag
     2761 cagggtgatc agcgtgtcca tgtcgtcctg gtcggcctcg gcgtggttgg tgtagcaggc
     2821 gtcgcagccc atcggcaggc ccagcagctt gccgcagaaa tggtcctcca gcgcggcgcg
     2881 gacgatctgc ttgccgtcat acagatattc cgggccgatg aagccgacga cggtgttcac
     2941 cagcagcggc gagaaggccc gcgccaccgc ataggcgcgc gcctccatgg tctgctggtc
     3001 cacgccgtga tgcgcctcgg cggacagcgc gctgccctgg ccggtctcga aatacatcac
     3061 gttattgccg atgctgccgc gctgcagcga cagcgccgcc tgctgtgcct cacgcagcag
     3121 gtccagcgtc acgccgaacc cggcattcgc cgcctgcgat ccggcgatcg actggaacac
     3181 caggtcaacc ggcgcaccgc ggcgaatgat ctccagcgtg ttggtgacgt gagtcagcac
     3241 gcagctctgc gtcgggacgt cgtggcgttg gcgcagcgcg tccagcatct ccagcaggag
     3301 catgcaggtg gggatgtcgt cgccggccgg gttgacgccg atcaccgcat cgccggtgcc
     3361 gtacagcagc ccgtccagcg tcgccgcggc gacgccgcgc agatcgtcgg tgggatggtt
     3421 gggctgcagc cgcgtgccca ggcggcccgg caggccctgg gtgttgcgga acgcggtggt
     3481 cacgcggcag cgcctggcga cggagatcag gtcctggttg cgcatcagct tgctgaccgc
     3541 ggccgccatc tccggcgtga tggctggcgc caggcgggcc agctgcgtgc cgtccgcctc
     3601 atacgacagc agccagtcgc ggaagtcgcc gaccgtcatg tgcgacagcg cggcgaaccc
     3661 ggcggcatcg tgggtgtcca ggatcagcct tgtcacgtcg tcggtttcgt acggcaccag
     3721 cggctcgtcc aggaagatgc gcagcggcag gtcggccagc aatgcgcggg cggcgacacg
     3781 ctgcgcatcc gagtctgcgg cgagcccggc cagttgatcg cccgaccgca gcggcgacgc
     3841 cttggccagc acgtcccgca ggtcgctgaa gcggtggcgt tcgccctgga agctgctggc
     3901 gtagctcggc atgcgtcggt tcccgcgatg gcttggtcgg gtgcgatgct agcacgctgc
     3961 ggcgtgcccg gtgcgatgca gacgatctgg cccgtagcgc agtcccgttt cgcggggact
     4021 tcgccgccgg gggcggtgat cctgctgcct cacgttccgg gtattcaagc tgttctgagt
     4081 ggggcagcgc ttggagtcac gcgtcgtcgt cacatcatcg ggtcatcaca gcatggggct
     4141 tgccccggag gatgacatgc gcggcagtca ggcagtgacc ggtgtggttc tggtggcggg
     4201 attcctcagc gggtgcgccg ccgaaccata cggctacagc tattcaccgg cttatgcgca
     4261 accctatccc tatgcgaact cgtactattc gccgttcact ctcaacttcg gcctaggcgc
     4321 cgcgcgggcg catcaccacc accaccatca tcatcaccat gccgtcgtcc gccatcatca
     4381 tcatcatcat catcaccatc accaccacgc gttccggcag aaggacgggt atggctgagc
     4441 gctgaacacc ctggcggttg tcccgctcgg cgccgccgcg agtccggcgg cgcccagggg
     4501 cgaggcggat gcgggcgcgg tggtggccgg ataaaaaaat ggcggcgctt tgcggcgccg
     4561 ccaagtttag ggaggaaacg tccaagaaag cagacagcgc ggtcagggac cgcagtgctg
     4621 cgaggcacaa actaggctcg cagtcgttcg tgtgcaagcg atatttcgca acgcagcatc
     4681 acgaaaatgg aacccctggc gctgccctac cttccgcggc tggaagcgcc ggggaccacc
     4741 aagggttgca tgccgtggct tcaccaagcc gcggggcggc tacgcgttga ctctgctgcg
     4801 ccggccgcta taacccgcgc tctccggccg ctgggcctcc ccgcggcccg cgctctgttg
     4861 ggaagctcgt catgttcgct gtcatccgca ccggcgggaa gcagtaccgc gtcacgccga
     4921 acgcggtgct caaggtggag aagctggaag ccgaacccgg cgccactgtc acctttaccg
     4981 atgttctggc ggtgggcggc gacggcggcc tgacgctggg cgccccgacc gtggccggcg
     5041 cctctgtggt ggcgaccgtc attgcccagg accggctgga caccgtcatc gtcttcaaga
     5101 agcgccgccg gcagaacagc cgccgcaaga acggccaccg ccagcgcgtc accgtgctgc
     5161 gcgtgtccgg catcggcacc gacggccagc ctccggctgc cgcggccgag caggcgtcgc
     5221 acgatccgga tccgcaggcg gcggacgtgg tggagaacct cgcggtggtg tccggggtgg
     5281 atcccgccgc ggtttccccg gtggaagagc cgaaggagta ggcagacatg gcacacaaga
     5341 aggcaggcgg ctcctcgcgc aacggccgcg ataccgaagg caggcgcctc ggcatcaaga
     5401 agttcggcgg cgagagcgtg atcgccggca acatcctggt ccgccagcgc ggcaccaagt
     5461 ggaagccggg ccagaatgtc ggcctcggcc gcgaccacac catcttcgcg ctggtcgacg
     5521 gcaaggtgga gttcagccgc aaggcggacg accgcgtgca cgtctcggtg cgaccgctgc
     5581 cggtggcggc cgagtagccg cgcggcaccc ggcgtatgag atgcaacggg ggtcggcggc
     5641 gccggccccc gtttttcgtt tggtaagcaa cccatgaagt tcctcgacca ggccaagatc
     5701 tacctgcgct ccggcgatgg cggcgacggc gtcgtcgcct tccggcgtga gaaatgcctc
     5761 gagttcggcg gccccgacgg cggcaacggc ggccgcggcg gcgacgtcgt gttcgaggcc
     5821 gccgccaacc tcaacacgct gatcgacttc cgctacacgc agcatttccg cgcccccaag
     5881 gggggcaacg gcgcgggttc gaaccgcacc ggcgcgggcg cgcgcgacgt ggtgatcgcc
     5941 gtccccatcg gcacggtgat cctcgacgag gaccgcgaga ccgtgctcgc ggacctggac
     6001 gcgccgggca agcgaatcgt cctgctccgc ggcggcgacg gcggccacgg caacgcgcac
     6061 ttcaagacca gcacgaaccg cgcccc
//
LOCUS       DASSZH010000456         5487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_863227, whole genome shotgun sequence.
ACCESSION   DASSZH010000456 DASSZH010000000
VERSION     DASSZH010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863227"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VFN42_09890"
     CDS             <1..811
                     /locus_tag="VFN42_09890"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8996967.1"
                     /translation="FGEMGLDAQVISPAPPQCYYNVPAQIGVEAARAVNNGIAEIMAQ
                     DPKRIPAAMGSVPLGAGGNAAAEELERAMTTLGLKGVEVLAHVGDKELSDPEFEPFWA
                     KAEQLGAVVFIHPSGFPQPERFRRFYFNNVIGNPLDTTVALHYLIFDGVLERYPNLKI
                     IAAHGGGYLPAYSGRIDHGWGARSDSHGDLPHPPSTYLKKIYFDTIVFTPQQLEAMVK
                     LFGADHVLLGTDYPYDMADYDPLGLLAACTELSAEQVSAIAGGNACGLFGF"
     gene            complement(944..1183)
                     /locus_tag="VFN42_09895"
     CDS             complement(944..1183)
                     /locus_tag="VFN42_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996968.1"
                     /translation="MSHEPCRSDCLSLAAHWRQRAADTGHSELGDAYRRIAESYERLA
                     KQALLCRRLVTAAAERRGEAVPCQCGIDGAIAGLG"
     gene            complement(1331..2242)
                     /locus_tag="VFN42_09900"
     CDS             complement(1331..2242)
                     /locus_tag="VFN42_09900"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HET8996969.1"
                     /translation="MTRRHRLVPGLLLTLLALLGGCATQPYCKLLPLAEMPVEDYRNL
                     LVVPAEINGQPVRLLVDTGAERTMLTETAVQRLRLGRDSHQTRSLGIGGVSAHFDARV
                     PGIWLGGTRFPVERVAVGAFALPELPGGPVDGLLGADILLAFDLDLNIPGQRLTLYRV
                     RRCPTAVPPWPALEVAGVGARRDRMLVPITVNGVAGKAVLDTGAQTSAISLEMAERTG
                     VSPATLNRDPKITVHGAAPAPVQVPIQRFSRMTIAGEPVLYPRLAVVPTMGGLGDGLV
                     GADFIRGRRLWLAFPTGQMFIAAGPAP"
     gene            complement(2280..2945)
                     /locus_tag="VFN42_09905"
     CDS             complement(2280..2945)
                     /locus_tag="VFN42_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HET8996970.1"
                     /translation="MRLESFRIRVWDAPTRLFHWAIVLLVATSWVTQELDWMRWHFLS
                     GYTLLAALLFRLAWGVVGSETARFSHFLRSPLAALRHLRELPRHRTDTIIGHNAAGGW
                     MVVVLLLLLGIQVGTGLCANDEISVQGPLADLVGDGWSNYLTHIHALNFKLIEVAIVL
                     HLLALAGYRLLGHDLLRPMITGWKRLPSSSASPRMANPLLALAIFVVAALAVAAGVRT
                     LGG"
     gene            3192..4307
                     /locus_tag="VFN42_09910"
     CDS             3192..4307
                     /locus_tag="VFN42_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET8996971.1"
                     /translation="MAQRIRHEDALTMPRLAQTPHPAAAPVAAKSGPAAPSFDIVRVN
                     PDGNAVLAGRAAPGAEVVVQQGDKEIGRSRADADGNWVLLPAAPLPPGAGELTLSEQT
                     AHGDRVKGERSVVLVVPPRAAPEQQASGTRVPAQTGGGKVPAAAVARAGMEPAQSTLL
                     EQASTKPAPSAPVEQTAMGKPAQPALALLTAGAQPPRVLQGPAAQPGSHRLQLGTVDY
                     GNSGQVRFAGTAPPNSTVRVYVDKQPVGDAKVQPDGTWTLTPPVDIPPGQHELRLDQL
                     ARQGGVGARIQLPFTRAQLTARQLAPGSVTVQPGQSLWLIARHSYGRGIRYTIIYKAN
                     QGHIRDPNLIYPGQIFTLPDAPEGSGVALRSATKASR"
     gene            complement(4232..4705)
                     /locus_tag="VFN42_09915"
     CDS             complement(4232..4705)
                     /locus_tag="VFN42_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996972.1"
                     /translation="MPFAAPFMLGPFLVDAQGRLAPRDAEALPAFLFRWRGCAIRARL
                     RPAQADRGRLVLRGVLGRIPSSGDATDLAGRAQCFTILRGLPRLLPQHWQLMLLPDHR
                     VLLESSAEVTLPITASGLLTELTVFLLTLAPYLDALVALRSATPEPSGASGSVKI"
     gene            4774..5445
                     /locus_tag="VFN42_09920"
     CDS             4774..5445
                     /locus_tag="VFN42_09920"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007006223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HET8996973.1"
                     /translation="MAFADDLRLALPGPHPAGRPFIIGGLVLLVIGLIFAGWLAWLAG
                     IFTLCCLYFFRDPHRVPPGRPGAIVAPADGRVVSVAPAPPPPELGLGPEPRWRVSIFL
                     TVLNVHVNRIPADGTVTRIAYHHGAFVNAAADKASELNERNAIAIRLHDGSELAVVQI
                     AGLIARRIVCTLREGDAVRSGERFGIIRFGSRTDLYLPQGLRPVVVEGQTMLGGETVI
                     AERLP"
BASE COUNT          913 a         1884 c         1873 g          817 t
ORIGIN      
        1 cttcggcgaa atggggctgg acgcgcaggt gatcagcccg gcgccgccgc agtgctacta
       61 caacgtgccg gcgcagatcg gcgtggaggc ggcgcgcgcg gtgaacaacg gcatcgccga
      121 gatcatggcg caggacccca agcgcattcc cgcggcgatg ggcagcgtgc cgctgggcgc
      181 cggtggcaac gccgcggcgg aagagcttga gcgtgcgatg accacactcg ggctgaaggg
      241 cgtggaagtg ctggcgcatg tcggcgacaa ggaactctcc gacccggagt tcgagccgtt
      301 ctgggcgaaa gcggaacagc tgggggcggt ggtgttcatc catccctcgg gctttccgca
      361 gcccgagcgt ttccgccgct tctacttcaa caacgtcatc ggcaatccgc tggataccac
      421 ggtggcgctg cactacctga tcttcgacgg cgtgctggag cgctatccga acctgaagat
      481 catcgcggcg catggcggtg gttacctccc ggcctattcc ggccggatcg atcacggctg
      541 gggcgcacgc agcgactcgc atggcgacct gccgcatccg ccgagcacgt atctgaagaa
      601 gatctacttc gacacgatcg tgttcacacc gcagcagctc gaggcgatgg tgaagctgtt
      661 cggcgcggat cacgtgctgc tgggcacgga ttatccgtac gacatggccg attacgatcc
      721 gcttggcctg ttggcggcgt gtaccgaact gagcgcggag caggtctccg cgatcgcggg
      781 cggcaacgcc tgcgggctgt tcgggttctg atgatggcgt gatggccggc gcaggccggt
      841 catccccgga gacctccggg catcaggtgc ttcgcagcgc cgaccgaccg gcggcaatcc
      901 cggggtggcc gggccggatc ccggccacga cacttgtgcc ttgctaaccc agcccggcga
      961 tcgccccgtc aatcccgcac tggcagggca ccgcctcacc gcgccgctcc gcggctgcgg
     1021 tcaccaggcg ccggcacaac agagcctgct tcgccagccg ctcatagctt tccgcgatcc
     1081 tgcgataagc atcccccagc tcggaatgtc ccgtatccgc tgcccgttgc cgccaatgcg
     1141 cggcaaggct gaggcagtcg gagcggcacg gttcatgtga catcggcgcc tcgcattgta
     1201 acgaagaccg tcgcacattc cgcgaaacgc ccccatgtcc gccttgattt ggatcaatca
     1261 cgagacaagt catgcgataa gcgaagtcat cttccgcatg gccgagtcga attcgggcaa
     1321 gattccagcc ttacggcgcg ggccccgcgg cgatgaacat ctggccggtg ggaaaggcca
     1381 accacaagcg gcgcccgcgg atgaagtcgg cgcccaccag cccgtcgccc agcccaccca
     1441 tggtcggcac gacggccagt ctgggataga gcaccggttc gccggcaatc gtcatgcggc
     1501 tgaaccgctg gatcggcacc tgcaccgggg ctggcgccgc gccatgcacg gtgatcttcg
     1561 gatcccggtt cagcgtcgcc ggcgacaccc cggttcgttc ggccatctcc agcgagatgg
     1621 cggaagtctg cgcgccggtg tccagtaccg cctttcccgc aacgccgttc accgtgatcg
     1681 gcaccagcat gcggtcgcgg cgcgcaccca cgccggccac ttccagggcc ggccagggcg
     1741 gcaccgcggt cgggcatcgg cggacgcgat acagcgtcag ccgctgcccg gggatgttca
     1801 gatccaggtc gaatgccagc aggatgtccg ccccgagcag gccgtccacc ggcccgcccg
     1861 gcagctcggg caaggcgaac gcgcccaccg caacgcgctc caccgggaat cgcgtgccgc
     1921 ccagccagat tccgggcacc cgcgcgtcga aatgcgcact gaccccaccg atcccgagcg
     1981 agcgtgtctg gtgagagtcg cgtcccagcc gcagccgctg gaccgcagtt tcggtcagca
     2041 tggtgcgttc ggcgccggta tcgaccagca gccgtaccgg ctggccgttg atctcggccg
     2101 ggaccaccag caggttccgg tagtcttcga ccggcatttc cgcgagcggc agcagcttgc
     2161 aatagggctg cgtggcacag ccgccgagca gggcgagcag cgtgaggagc aggccgggaa
     2221 ccagccggtg ccgtcgtgtc atgctgtcgg ctgcagcctg cgggaggtgt ctgtcatgct
     2281 cagccgccaa gcgtccgcac accggccgcc acggccagtg ctgcgacaac gaagatcgcc
     2341 agggccagaa gcgggttggc catgcggggc gatgcggaac tcgacggcag ccgcttccag
     2401 ccagtgatca tcggccgcag caggtcgtgg cccagcaagc ggtaacccgc cagcgccagc
     2461 aggtgcagga cgatggcgac ttcgatcagc ttgaagttca aggcgtggat atgggtgagg
     2521 taattgctcc agccgtcgcc aaccaggtcg gcgagcgggc cctgcaccga aatctcgtca
     2581 ttcgcgcaaa gcccggtgcc aacctgaatc cccagcagca gcagcaggac caccaccatc
     2641 cagccgccag cggcgttgtg gccgatgatg gtgtcggtgc ggtggcgcgg cagttcccgc
     2701 aagtggcgca gcgcggcaag cgggctgcgc aggaaatggc tgaagcgtgc tgtttcactg
     2761 cccaccactc cccaggccag gcggaacagc aaggccgcca gcagcgtgta gccggacagg
     2821 aaatgccatc gcatccagtc gagttcctgg gtcacccaac tggtcgccac cagcagcacg
     2881 atcgcccaat ggaacagccg ggtgggcgcg tcccagacgc gaatcctgaa agactcaagg
     2941 cgcatccgcg ggcttctccg tcgcttgccg gctggccgtc ccagcctcgg ccgggtcagc
     3001 cgcttccttg cccatccgcg tgttcggtgc aaggactgtg ctgaggacaa tgttgtgccg
     3061 tcacgaaaaa tgcctcaagc taaggaatga tggcgagctc caggtttttt ccaccgcggt
     3121 ctccaatact gcgcacgatt ctggcgctgc tgctggttgc tgccgtcgtg gtcaccgcga
     3181 gcacgatctg gatggcgcag cgcattcgcc atgaagatgc actgaccatg ccgcgtttgg
     3241 cgcaaacgcc gcatccggcg gctgccccgg tagcagcgaa gtccggcccg gcagcgccca
     3301 gcttcgacat tgtccgcgtc aatccggacg gcaatgcggt gctggccggc cgcgccgcgc
     3361 cgggcgccga ggtcgtggtg cagcagggcg acaaggagat cggccggagc cgcgccgatg
     3421 cggatggaaa ctgggtgctg cttcccgccg cgccattgcc gccgggcgcc ggcgagctga
     3481 ccctgtccga gcagaccgcg cacggcgatc gggtgaaggg cgagcggtcc gtcgtcctgg
     3541 ttgtgccgcc gcgggcggcg cctgagcagc aggccagcgg gacgcgcgtc ccggcgcaga
     3601 cgggcggagg gaaagtcccg gccgcggcgg tcgcgcgggc cggcatggag cccgcccaat
     3661 ccacgctgct ggagcaggcc agcacaaagc ctgccccgtc tgcgccggtg gagcagaccg
     3721 cgatgggcaa gcccgcccag ccggcgctgg ccttgctgac tgccggggcg cagccgccgc
     3781 gcgtgctgca gggaccggcc gcccaaccag gctcgcaccg gctgcaactt ggcacggtgg
     3841 actacggcaa ttccgggcag gtccgttttg ccggtaccgc gccgccgaac agcacggtgc
     3901 gggtctacgt ggacaagcag ccggtgggtg acgccaaggt gcagccggac ggaacctgga
     3961 cgttgacgcc tccggtcgac atcccgccgg gccagcacga attgcggctg gatcagcttg
     4021 ccaggcaggg cggcgtcggc gcgcggatcc agcttccttt cactcgggcg caactgaccg
     4081 ccaggcagct cgcgccgggc agcgtcacgg tgcagcccgg gcaaagcctg tggctgatcg
     4141 cccggcacag ctatggccgg ggaatccgct acaccatcat ctacaaggcg aaccagggcc
     4201 acatccgtga tccgaacctg atttacccgg gtcagatctt cacgcttccg gacgcgccgg
     4261 agggctccgg cgtggcgctg cgcagcgcca ccaaggcatc caggtagggt gccagcgtca
     4321 gcaggaacac ggtcagctcg gtcagcaggc cggatgcggt gatcggcagt gtcacctccg
     4381 cgctggattc cagcaggacg cgatggtccg gcagcagcat gagctgccag tgctgcggca
     4441 gcagccgcgg caatccgcgc aatatggtga agcactgggc tcggccggca aggtccgtcg
     4501 cgtcacccga gctggggatg cggcccagca cgccgcgcaa caccagccgg ccgcggtccg
     4561 cttgcgccgg gcgaaggcgg gcgcggatcg cgcagccgcg ccagcggaac aggaaggcgg
     4621 gcagcgcctc ggcgtctcgc ggcgcaaggc ggccctgggc gtccaccagg aagggaccga
     4681 gcatgaacgg cgcagcgaac ggcatggggc gactcccggc ttgccacggg agtcttgccg
     4741 gcaattgatg aagcatccat aaaccgcgca atcatggcct tcgctgacga cctccgcctg
     4801 gcactgccgg ggccgcatcc tgccggaaga cccttcatca tcggcgggct ggtgctgttg
     4861 gtgatcggcc tgatcttcgc cggctggctg gcctggctgg caggtatctt caccctgtgc
     4921 tgcctgtact tcttccggga cccgcaccgc gtgccacccg gccggcccgg tgcgatcgtg
     4981 gcgcccgcgg acggacgcgt ggtctcggtg gcgccggcgc cgccgccgcc ggaactcggc
     5041 ctgggaccgg agccgcgctg gcgggtgtcg atcttcctga cggtgctgaa cgtgcacgtg
     5101 aaccgcattc cggccgacgg cacggtcacc cgcatcgcct accatcacgg agccttcgtg
     5161 aacgccgccg ccgacaaggc gagcgagctg aacgagcgca acgccatcgc catccgcctg
     5221 catgacggca gcgaactcgc cgtggtgcag atcgccgggc tgatcgcccg ccgcatcgtc
     5281 tgcaccttgc gggaaggtga cgctgtgcgg tcgggggagc gctttggtat catccgcttc
     5341 ggcagccgca ccgatctgta cctgccccag gggctgcgcc ccgtggtggt ggaaggccag
     5401 acgatgctgg gcggcgaaac agtgatagcg gaacgccttc catgagcgat cccggcacgc
     5461 ctccacgccc ggcccgtccg ggcccca
//
LOCUS       DASSZH010000457         2810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_232510, whole genome shotgun sequence.
ACCESSION   DASSZH010000457 DASSZH010000000
VERSION     DASSZH010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232510"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1538
                     /locus_tag="VFN42_09925"
     CDS             <1..1538
                     /locus_tag="VFN42_09925"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET8996974.1"
                     /translation="LFDAALETRLDLVHTRHEAAAVHMADAYGRLTGEPGIAMVTGGP
                     GHANAVSALFTALGQESPMLLLSGHTETDQLNRGGFQELRQAEMAAPVSKASWTASDP
                     EALGEDVAKALRIAKSGRPGPVHLSLPSDLLDARVAGNAIRFPEASAFAAMPVPLHDA
                     AADAVLRMLAEAKRPLLIAGPQLANRGGRDLLARLESSLQIPTCIMESPRGFNDATLG
                     AFSDVAKRADLIILLGKALDFTLKFGAAPAIDPACRFVVIDPEAPIIDRALRERGEQI
                     AFACVADTRPAGEALLRRAQGKADAGWLREARACFTDRPDSWGALTSQTPGRLHAVEV
                     FRALKPYVERDPDTVLICDGGEFSQWGQSMLPLLPRRIINGVGGSIGSSLPMANAARL
                     VEPNAPVFAVLGDGTFGFHMAEFETAVRRNLPFVAVVGTDARWNAEHNLQIRDYGRDR
                     AHGCDLLPTRYDRVVEALGGHGEFVDRADQMPAAIERALASGKPACINVMIESIPAPL
                     LRR"
     gene            complement(1727..2476)
                     /locus_tag="VFN42_09930"
     CDS             complement(1727..2476)
                     /locus_tag="VFN42_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8996975.1"
                     /translation="MGKLAGKVALVTGSGRNIGRATVLKLAGEGAHVVVNARSNQAEA
                     DAVVREARALGVKAMAIVADIGRKDQVEAMAAQALSEFGRVDILINNAAIRPHKPFTE
                     LTAQDWEAVRSVVLDGALYLTRALIEPMVANRYGRILFFTGEGAFTGGSGRAHVSAAK
                     MGLVGFARGLASEFAPHNIRVNVVSPGIIDTARANPEWYQGRLPNAAGIPLGRQGRIE
                     EIAATCLFLVSDDGGFITGQTIHVNGGSAYY"
     gene            complement(2506..>2810)
                     /locus_tag="VFN42_09935"
     CDS             complement(2506..>2810)
                     /locus_tag="VFN42_09935"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET8996976.1"
                     /translation="SVADSKAWEKRLNDAGAPCASIWRIEEVIDHPQIAARDVLQTAE
                     TPYGAVRLMGSGFKLAHGGGRLDSAPPELGAHTDAILRAAGSSAEDIAALRQNGVI"
BASE COUNT          446 a          953 c          928 g          483 t
ORIGIN      
        1 cgctgttcga tgccgcgctg gagaccagac tcgaccttgt gcacacgcgg catgaagccg
       61 ccgcggtgca catggcggat gcgtacgggc ggctgaccgg cgaacctggc atcgccatgg
      121 tcaccggcgg tcccggccat gccaacgccg tctccgcgct gttcaccgcg ttgggccagg
      181 aatcaccgat gctgctgcta tcgggccaca cggagaccga tcaactcaat cgcggcgggt
      241 tccaggaact gcgccaggcg gaaatggccg cgccggtcag caaagcgtcc tggaccgcga
      301 gcgatccgga ggccctgggg gaggacgtcg ccaaggctct gcgcatcgca aaatccggcc
      361 gtcccggacc ggtgcatctc agcctgccgt ccgacctgct ggatgcgcgc gtcgccggca
      421 atgcgatccg ctttccggag gcgagcgcct tcgccgccat gccggtgccg ctgcacgatg
      481 ccgcggcgga cgcggtgctg cgcatgctgg cggaggcaaa gcggccgctg ttgatcgccg
      541 gcccgcaact ggcgaaccgc ggcggacgcg acctgctcgc ccggctcgaa agcagtttgc
      601 agattccgac ctgcatcatg gagagcccgc gcgggttcaa cgacgccacc ctgggcgcct
      661 tttccgatgt cgcgaagcgc gccgacctga tcatcctgct gggcaaagcg ctggacttca
      721 cgctgaaatt cggtgccgcc ccggcgatcg atcctgcctg ccgcttcgtg gtgatcgacc
      781 cggaagcgcc gatcatcgat cgcgcgctgc gcgagcgtgg cgaacagatc gcctttgcct
      841 gcgtcgccga cacgcgtccc gccggcgagg cgctgttgcg ccgggcgcag ggcaaggcgg
      901 atgccggctg gctgcgggaa gcacgtgcct gcttcaccga tcggccggac tcctggggtg
      961 cgttgacatc gcagaccccc ggcagactgc atgcggttga agtcttccgt gcattgaagc
     1021 cctatgtcga acgtgatccg gatacggtgc tgatctgtga cggcggtgag ttctcacaat
     1081 ggggacagag catgttgccg ctgctgccgc gccgcatcat caatggcgta ggtggctcga
     1141 tcggcagttc cctgccgatg gcgaatgcgg cccgcctggt cgaaccgaac gcaccggtgt
     1201 ttgcggtgct gggcgacggt acgttcggct tccacatggc ggaattcgaa accgcggtgc
     1261 gccgcaacct gccctttgtc gccgtggtcg gcacggatgc gcgctggaac gcggagcaca
     1321 atctgcagat ccgcgattac ggccgcgacc gcgcgcatgg ctgcgacctg ctgcccacgc
     1381 gctacgatcg cgttgtcgag gcgctgggcg gtcacggcga atttgtggat cgcgcggacc
     1441 agatgccggc cgcgatcgag cgcgcgctgg ccagcggcaa accagcctgc atcaacgtga
     1501 tgatcgaaag catcccggcg ccgctgctcc gccgttgaac gcctcatacc agcggccgaa
     1561 agtctcgacg cttctgtgtc atgcacggcc ttggcccgtg catccacgtc ttgcggtagc
     1621 gcacgcaaca gagacgtggg tggccgggcc aagctccggc catgacacgg gggaggaaac
     1681 gctacgccgg aacgggtcag acctatggtc cgctggtata tatatatcag taatacgcgc
     1741 tgccgccgtt cacgtggatg gtctggccgg tgataaagcc gccatcgtcg ctgaccagga
     1801 acaggcaggt ggcggcaatc tcctcgatcc tgccctggcg tcccagcgga atgccggcgg
     1861 catttggcag gcggccctgg taccattcgg gattggcgcg cgccgtgtcg atgatgccgg
     1921 gcgagaccac gttcacccgg atgttgtgcg gcgcgaattc ggaagccagc ccgcgcgcga
     1981 aaccgaccag ccccatcttc gccgcggaaa catgggcccg cccgctgccg ccggtgaagg
     2041 cgccctcgcc ggtgaagaac aggatgcggc cgtagcggtt cgccaccatc ggttcgatca
     2101 gtgcgcgcgt caggtacagc gcgccatcca gcaccacgct gcgtaccgct tcccagtcct
     2161 gcgcagtcag ctcggtaaag ggcttgtgcg gccggatcgc cgcgttgttg atcaggatat
     2221 cgacgcggcc gaattccgac agcgcctgcg ccgccatcgc ttccacctgg tccttgcgcc
     2281 cgatatcggc gacgatcgcc atcgccttga cgcccagcgc gcgggcttca cgcacgacgg
     2341 cgtcggcctc ggcctggttg ctgcgcgcgt tcaccacgac atgcgcgcct tcgccggcca
     2401 gcttcaatac cgtggcgcgg ccgatattgc ggccggagcc ggttaccagc gcgaccttcc
     2461 cagcaagctt ccccacgtcg tttctccctt gttggagcga ccggttcaga tcactccgtt
     2521 ttgccgcagc gcggcgatat cctcggccga ggagccggcc gcccgcaata tagcgtcggt
     2581 gtgcgcgccc agttcaggtg gtgcactgtc cagccggccg ccgccatgcg ccagtttgaa
     2641 tccggatccc attagccgca ccgcgccata gggcgtttcc gctgtctgca gcacgtcccg
     2701 cgcggcgatc tgcggatggt cgatgacttc ctcgatgcgc cagatgctgg cgcagggagc
     2761 gccggcatca ttcaggcgct tttcccaggc cttggagtcg gcgacggata
//
LOCUS       DASSZH010000458         2968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1408433, whole genome shotgun sequence.
ACCESSION   DASSZH010000458 DASSZH010000000
VERSION     DASSZH010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408433"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="VFN42_09940"
     CDS             <1..1174
                     /locus_tag="VFN42_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996977.1"
                     /translation="LDASAGAGRTEDLAADGSGEVATARGIDAAKEAFLKACGRGESN
                     KADHLFLWLWQHVTPIEAFDLLMSVAIPKNALDDHYFLFPAYTWRMLDSYLGWEHMQV
                     LMRPAVRYVARFPTQRSVPEIDALIEKHGLLTRILRQRTGDDETAEIGRIGEAIRACA
                     SYNEIPAMLAEALANGLSLEGAGEALSIGAAGLFLRSLTGNPMDVHLHTSANIRRWLL
                     RLDGLSLRNKLGVLLLWHTGPEVKSTQYRMEPAPQPDPAAVAALPHRSQEDLLEAITR
                     SIYEQPPTDWSTVTNLGRMRAVPEVKITVNLAQQYVDLRYDPEVLMRRLGEIVCHDNF
                     TEMHAFKHHQAIVEEFHTTREPWRWMHLVCGCQAAAISFGKNMEVFEEALELMHAA"
     gene            1423..1674
                     /locus_tag="VFN42_09945"
     CDS             1423..1674
                     /locus_tag="VFN42_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019334760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HET8996978.1"
                     /translation="MTTLTVTAKGQVTLRKDLLQHLGVGPGQKISVEKLPDGRIEVKA
                     ATPQGRISDVFNLLHRAGAPSLSIEQMARIAAKGWAGEK"
     gene            1671..2069
                     /locus_tag="VFN42_09950"
     CDS             1671..2069
                     /locus_tag="VFN42_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017170488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HET8996979.1"
                     /translation="MKITADTNVLVRALTGDDARQSKAAQAELASAEMTALTLPTLCE
                     LVWVLSRGYKIPAAEIAEAIRRLTNATTAVVNRPAVAAGLAMLEAGGDFADGVIAYEG
                     SWSGSEAFVSFDRHAVTLLQQQGQAARLLR"
     gene            complement(2083..>2968)
                     /locus_tag="VFN42_09955"
     CDS             complement(2083..>2968)
                     /locus_tag="VFN42_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996980.1"
                     /translation="DTLCHRIPRFIEKAGRAGVVRVFIGLESINPESLLAAKKKQNRI
                     AEYRDMLLAWKRIRAVIWAGYIIGFPGDTPETVMRDIRIIQHELPIDFLEFFVLTPLP
                     GSEDHQKLALAGTPMDADLNRYDTFHVVTAHPAMRREEWEHTYWAAWRAYYTFDHMCT
                     LMRRAAATRISLGKMLSMLLSFWSLSLIEGTHPLEGGYFRRKIRTERRPGLPIVPAWR
                     FWPQFALELVTKHARISAMAVRLLLLRHRLKRDKTARDYRDLALTPVSDESLEMLTVT
                     EAARTAARTRLRPRALAG"
BASE COUNT          524 a          962 c          984 g          498 t
ORIGIN      
        1 gctggatgcc agcgccgggg ccgggcgcac cgaagacctg gcggccgacg ggtcgggcga
       61 agtggcgacc gcgcgcggca tcgatgccgc caaggaggcc ttcctgaagg cctgcggccg
      121 cggcgagagc aacaaggccg atcacctgtt cctgtggctg tggcagcacg tgacgccgat
      181 cgaggcgttc gatctgctga tgagcgtcgc catcccgaag aacgcgctgg acgaccatta
      241 tttcctgttc cctgcctata cgtggcggat gctggattcc tatttgggct gggagcacat
      301 gcaggtgctg atgcggccgg cggtgcgcta cgtggcgcgc ttcccgacgc agcgctcggt
      361 gccggaaatc gatgcgctga tcgaaaagca tggcctgctc acgcgcatcc tgcgccagcg
      421 caccggcgat gacgaaacgg cggagatcgg ccgcatcggc gaagcgatcc gcgcctgcgc
      481 ctcctacaac gaaatcccgg cgatgctggc ggaagccctg gccaacggcc tgtcgctgga
      541 aggcgccggc gaagcgctgt cgatcggtgc ggccgggctg ttcctgcgct cgctcaccgg
      601 caacccgatg gatgtgcacc tgcacaccag cgccaatatc cgccgctggc tgctacgcct
      661 ggacggactc agcctgcgca acaagctcgg cgtcctgctg ctgtggcaca ccgggccgga
      721 ggtgaagtcc acccagtacc gcatggaacc ggcaccgcag cccgatcccg cggccgtcgc
      781 cgcgctgccg caccgcagcc aggaagacct gctggaagcg atcacaagaa gcatctacga
      841 acagccgccg accgactggt ccaccgtgac caacctgggg cgcatgcgcg cggtgccgga
      901 ggtgaagatc accgtgaacc tggcacagca atacgtcgat ctgcgctacg acccggaggt
      961 gctgatgcgc cggttgggcg agatcgtctg ccacgacaac ttcaccgaga tgcatgcctt
     1021 caagcatcac caggcgatcg tcgaggaatt ccacaccacg cgcgaaccgt ggcgctggat
     1081 gcacctggtc tgcggctgcc aggccgcggc aatctccttc ggcaagaaca tggaggtgtt
     1141 cgaggaggcg ttggagctga tgcacgcggc gtagctgcaa cgccggaagg cggccttccg
     1201 gcgttgcgga taggctaagg cggcgcgcag gaataaccac gacgcaccgg ccggcttgcg
     1261 ccacctcgtc acggtcggcg atgggtgacc atgacggaac ggagcaggct gccgccagcc
     1321 gaatcgccta tttctgcgtg tcgcctgacg catcgttctg gccgctcttg tgtgtaaggc
     1381 taattcgcct tacatgcccg gcgtaccagg aacggaagtg ccatgaccac cttgactgtc
     1441 accgcgaagg gccaggtaac gctccgcaag gatctgctgc agcatctggg cgttggcccc
     1501 ggccagaaga tcagcgtgga gaagcttccg gacggtcgca tcgaggtcaa agccgccacc
     1561 ccgcagggcc ggatctccga cgtattcaac ctgttgcatc gtgccggtgc gccttcgctg
     1621 tccattgagc agatggcccg gatcgccgcc aagggttggg cgggggagaa atgaagatca
     1681 ccgcggacac caatgtcctg gtgcgcgctt tgaccggcga tgacgcccgt cagagcaaag
     1741 ctgctcaggc cgaactggct tcggccgaga tgaccgcgct gactttgccc actctctgtg
     1801 aactcgtatg ggttctttca cgcggctaca agataccagc ggcggagatt gccgaggcga
     1861 tccggcgcct caccaacgcc acgaccgccg tggtgaaccg gccggcggtg gcggctgggc
     1921 ttgccatgct tgaagccggc ggcgatttcg ccgatggcgt tatcgcgtat gaagggagtt
     1981 ggtccggcag cgaagcattc gtctcgttcg accggcacgc tgtcacgctg ctccagcagc
     2041 agggacaagc ggcgcggttg cttcgttagt ggcgcgcggc cgctaccccg ccagcgcccg
     2101 cggccgcagc cgggtgcgcg cggcagtgcg cgccgcttcg gtcacggtga gcatctccag
     2161 cgattcgtcg ctcaccggcg tcagcgccag gtcacgatag tcacgcgccg tcttgtcgcg
     2221 tttcaggcgg tgccgcagca gcagcaggcg cacggccatc gccgaaattc gcgcgtgctt
     2281 cgtcaccagt tccagcgcga attgcggcca gaaacgccag gcaggaacga tgggcagtcc
     2341 cgggcggcgc tcggtgcgta tcttgcgccg gaaataaccg ccttccagcg gatgcgtgcc
     2401 ttcgatcagg ctgagactcc aaaaactcag tagcatactc agcattttgc cgagcgaaat
     2461 gcgcgtggcg gcggcgcggc gcatcagcgt gcacatgtgg tcgaaggtgt agtaggcgcg
     2521 ccacgccgcc caataggtgt gttcccattc ctcccgccgc atcgccggat gcgcggtgac
     2581 cacgtggaac gtgtcgtagc ggttgaggtc ggcatccatc ggcgtgccgg ccagcgccag
     2641 tttctggtga tcctccgagc cgggcaacgg cgtcagcacg aagaactcca ggaagtcgat
     2701 cggcagctcg tgctggatga tgcggatgtc gcgcatcacc gtctccggcg tgtcgcccgg
     2761 gaagccgatg atgtagccgg cccagatcac cgcgcggatg cgcttccagg ccagcagcat
     2821 gtcgcgatat tcggcgatgc gattctgctt cttcttggcc gcgagcaggc tttccggatt
     2881 gatcgattcc aggccgatga acacccgcac cacgccggcg cgcccggcct tttcgatgaa
     2941 gcgcgggatg cgatggcaca gcgtgtcc
//
LOCUS       DASSZH010000459         2717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_113198, whole genome shotgun sequence.
ACCESSION   DASSZH010000459 DASSZH010000000
VERSION     DASSZH010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113198"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /locus_tag="VFN42_09960"
     CDS             complement(<1..1211)
                     /locus_tag="VFN42_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HET8996981.1"
                     /translation="MNIVPSGDSLRPLPPPEVPPPPPRSRLGAVADILLGKTVTITLT
                     AIALLVAVATFIILARGTQPFGLPPGYGIGLVLANLCCLLLLGAVLAGRLTRVWVERR
                     RGSAGSRLHVRLVMLFSGVAVAPTIVVACFAVAFFHYGIQAWFNDPVRESINESLQVA
                     RGYLEEHRENIRAVALEMANDLARAGVFLAADPNVFADVLGTQTTLRGLTEAIIYDPS
                     TDQVMAAAGLFSMGVKPPAQSFTQQALHGDVVVMNSPDGTRVRALVKLDSVPPLILMI
                     GRPVDPQILGYMKRTEQAVSEYQRLMANRSWLQIAFAWIFAIVALLVLAAAVLIGLVL
                     ANQIARPIGTLINAAERVRSGDLRVRVAEIATGDEVATLSRAFNRMTGQLAAQRTELM
                     EAYSQLDERRR"
     gene            complement(1228..2685)
                     /gene="ntrC"
                     /locus_tag="VFN42_09965"
     CDS             complement(1228..2685)
                     /gene="ntrC"
                     /locus_tag="VFN42_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HET8996982.1"
                     /translation="MTPPTVLIADDDRSIRTVLTQALGRAGYQVRSTGHAATLWRWVE
                     DGEGDLVITDVVMPDENGLDLIPRIRRIRPDLRVVVMSAQSTLITAVKAAQRGAFEYL
                     PKPFDLDELLSVVGRALAVPPVPIEVAAEPRDADERLPLIGRSPAMQEVYRTIARLST
                     ADLTVMINGESGTGKELVARALHDYGKRRAGPFVAINMAAIPRELIESELFGHERGAF
                     TGATTRSQGRFEQANGGTLFLDEIGDMPPEAQTRLLRVLQEGEFTSVGGRQPIKANVR
                     IIAATHRDLRNAIRQGSFREDLFYRLNVVPIRLPPLRERAEDIPLLARHFLERAHVDG
                     LPLKSLDQGAVDRLKQYSWPGNVRELENLMRRLAALCPDETIGAEAVAPELAETASPP
                     AVAAAAQGQAGPEPLAQAVERHIRSFLAAHSDGIGPSNVYDRVIAEVERPLIRLTLAA
                     TRGNQIKAAAMLGLNRNTLRKKIRDLDIPVVRGLV"
BASE COUNT          434 a          947 c          932 g          404 t
ORIGIN      
        1 aagcgccggc gttcgtcgag ctggctgtac gcctccatca gctcggtacg ctgcgcggca
       61 agctggccgg tcatgcggtt gaacgcgcgc gacagcgtgg caacctcgtc gccggtggcg
      121 atttccgcca cccgcacgcg cagatcgccg ctgcgcaccc gctcggcggc attgatcagc
      181 gtgccgatcg ggcgcgcgat ctggttcgcc agcaccaggc cgatgagcac cgcagccgcc
      241 agcaccagca gcgccacgat ggcgaagatc caggcaaagg cgatctgcag ccaggagcgg
      301 ttggccatca gccgctgata ttccgagacc gcctgctcgg tgcgcttcat gtagccaagg
      361 atctgcggat cgaccggacg gccgatcatc aggatcagcg gcggcaccga atccagcttc
      421 accagcgcgc gcacccgcgt gccgtccggc gagttcatca ccaccacgtc gccgtgcagc
      481 gcctgctggg tgaagctctg tgccggcggc ttcacgccca tgctgaacag cccggccgcc
      541 gccatcacct gatcggtgct gggatcgtag atgatcgcct cggtcaggcc gcgcagcgtg
      601 gtctgggttc ccagcacgtc ggcgaacacg ttcggatcgg cggccaggaa cacgccggca
      661 cgcgccaggt cgttggccat ttccagcgcc acggcacgga tgttctcgcg gtgctcctcc
      721 aggtagccgc gggcgacctg cagcgattcg ttgatcgact cgcgcaccgg gtcgttgaac
      781 caggcctgga tgccgtaatg gaagaacgcg acggcgaagc aggcgaccac gatggtgggt
      841 gcgacggcga cgccgctgaa cagcatcacc agccggacat gcaggcgtga cccggcggag
      901 ccgcggcggc gttccaccca gacgcgggtc aaccggccgg cgagcacggc ccccagcagc
      961 agcaggcagc acagattcgc cagcaccagc ccgatgccgt agccgggcgg cagtccgaag
     1021 ggctgtgtgc cacgggcgag aatgatgaag gtggcgacag cgaccagcag cgcgatggcg
     1081 gtcagcgtga tggtaaccgt cttgccgagc aggatgtcgg caaccgcgcc gaggcgggag
     1141 cgcggcggcg gcggcggcac ctcgggcggt ggcagcggac gcagcgagtc gccggaaggg
     1201 acgatgttca tgctgcgcgc ccgccgctca caccaacccc cgcacgacgg ggatatccag
     1261 gtctcggatc ttcttgcgca gcgtgttgcg gttcagtccc agcattgccg cagccttgat
     1321 ctggttgccg cgggtggcgg cgagggtcag gcggatcagc gggcgttcca cctcggctat
     1381 cacccggtcg tagacgttgg atggcccgat cccgtcgctg tgggcggcga ggaaactacg
     1441 gatgtggcgc tccaccgcct gggccagcgg ctcgggtccg gcctggccct gcgccgcagc
     1501 cgcgaccgcc ggcggcgatg cggtttcggc cagctccggc gcgacggctt ccgcgccgat
     1561 cgtctcgtcc gggcacagcg cagccaggcg gcgcatcagg ttctccagct cgcggacatt
     1621 ccctggccaa ctgtactgct tcagacggtc caccgcgccc tggtcgagcg atttcagcgg
     1681 cagcccgtcc acatgggcgc gttccaggaa atgccgcgct agcaggggaa tgtcctcggc
     1741 gcgttcgcgc agcggcggca ggcggatcgg cacgacgttc agccggtaga acaggtcctc
     1801 gcggaagctg ccctggcgga tcgcgttgcg caggtcgcgg tgggtggcag cgatgatgcg
     1861 cacattggcc ttgatcggct ggcgcccacc gacgctggtg aactccccct cctgcagcac
     1921 gcgcagcagc cgggtctgcg cctccggtgg catgtcgccg atctcgtcga ggaacagcgt
     1981 gccgccgttg gcctgctcga agcggccctg gctgcgcgtg gtggcgccgg tgaaggcgcc
     2041 gcgctcatgg ccgaacaact cgctctcgat cagctcgcgc gggatcgccg ccatattgat
     2101 cgcgacgaag ggtccggcac gccgcttgcc gtagtcgtgc agcgcgcggg cgaccagctc
     2161 cttgccggtg ccggactcgc cgttgatcat caccgtcagg tcggcggtgc tgagccgcgc
     2221 gatggtgcga tagacctcct gcatcgccgg gctgcggccg atcagcggca ggcgctcgtc
     2281 ggcatcccgc ggctcggccg ccacctcgat cggcaccggc ggcaccgcca gcgcgcggcc
     2341 caccacgctc agcagctcat cgagatcgaa cggcttcggc aaatactcga acgcgccgcg
     2401 ctgcgccgcc ttcaccgcgg tgatcaacgt cgattgcgcg ctcatcacca ccacccgcaa
     2461 atcgggacgg atgcggcgga tgcgcggaat cagatcgagg ccgttctcgt ccggcatcac
     2521 cacgtcggtg atcaccagat cgccctcgcc atcctccacc caccgccaca acgtcgcggc
     2581 atggccggtc gaacgcacct gatagccggc acgccccaac gcctgcgtca gcacggtgcg
     2641 gatcgaacga tcgtcatcgg caatcaacac ggtcggcgga gtcatcggca tgtcctgacg
     2701 cggaggggga acgggtg
//
LOCUS       DASSZH010000460         3077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1596138, whole genome shotgun sequence.
ACCESSION   DASSZH010000460 DASSZH010000000
VERSION     DASSZH010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596138"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VFN42_09970"
     CDS             <1..645
                     /locus_tag="VFN42_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996983.1"
                     /translation="PGATAAPAPYVRLAYEWDWNDQAAHVGGVLLAANLNPAAGPRQG
                     DSSAGQDHYLDIGVDAGYQFLGSGRHVVSTDLLVLHENRKLNGSAALGSVANPNGRLN
                     EIRASATYFFQATYGLTLAWQNIWGTADPVLLAPAPVTGSRNGSPNSNAFVIEADWVP
                     FGKETSWARPFANLKIGVQDTIYTRFNGSGSNYDGFGRRAGANNTLFAYAWLAF"
     gene            complement(662..1384)
                     /locus_tag="VFN42_09975"
     CDS             complement(662..1384)
                     /locus_tag="VFN42_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HET8996984.1"
                     /translation="MLLAYVDGELDAAGVREVEQLLANDPQARRTVEMYRETAGLLRA
                     ACSETVYAADAARLLPPALPRAWPRRRLIWAAAACLVLGIAAGAGGTWLGTRQSAMAA
                     LIDEIADYHSVYSRERGHLVEVPAAQAQEFVAWLDKRLHRHLEIPDLSAEGLSFAGGR
                     MLVVDGRPVAQLMYTRAQGLPVALCVSQMEGGSRPLRVERHDALRVAAWQQDGYAYVV
                     VGEMPADLARDIAERARAQLHA"
     gene            complement(1413..1961)
                     /locus_tag="VFN42_09980"
     CDS             complement(1413..1961)
                     /locus_tag="VFN42_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET8996985.1"
                     /translation="MAEDLRPMLSALLPRLRRFGLALTGSAVEADELVQDACARALQR
                     EGQLRDQTRLDAWMYGIMRNLWIDEMRTRSRRRHEPLDVATEVAGDDGPAVAEGRITL
                     ELVRRRLQEMPAEQRTVLILVCVDGLSYREAADVLGVAIGTVMSRLSRARRDLAERLG
                     ARLSGSVSLFPGPNGRSTFRSM"
     gene            complement(2387..>3077)
                     /locus_tag="VFN42_09985"
     CDS             complement(2387..>3077)
                     /locus_tag="VFN42_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8996986.1"
                     /translation="LLRLPVPLTGAAALRADVPADAATWAPFFLRALAEEAADARQML
                     ADLECSWIMARRRVASRRRDSHVAAAVDLLAAVPLLSATSLAGILGVAVKTAIRLLDE
                     LAAAGIAVEVTHRAKRRLFGLTGMAPLRDAVRPPYRPEPGRGRGRPPRRVVTPEAEAP
                     PPLPPLSPLEGQGFDYSDLEAGLAHLDFALRRTRQALDALARTPGRTVAVEASDTLSP
                     QQAVDSAEATG"
BASE COUNT          508 a         1090 c         1032 g          447 t
ORIGIN      
        1 cccggtgcca cggcggcccc ggcgccgtat gtgcggctgg cctatgaatg ggactggaac
       61 gaccaggcgg cgcatgtggg cggagtgctg ctcgccgcga acctcaaccc cgcggccgga
      121 ccgcgacagg gcgacagcag cgccggccag gaccattatc tcgatatcgg ggtcgatgcc
      181 ggttatcagt tcctcggttc agggcgccac gtcgtcagca cggacctgct ggtgctgcac
      241 gaaaaccgta agctgaacgg gtccgcggcg ctggggagcg tcgccaatcc caacggccgg
      301 ctcaacgaga tccgcgcgag cgcgacatac ttcttccagg caacctatgg cctgaccctg
      361 gcgtggcaga atatctgggg cacggccgat ccggtgctgc tggcgcctgc ccccgtcacc
      421 ggcagccgta acggcagccc gaacagcaac gccttcgtca tcgaggccga ctgggttccg
      481 ttcggcaagg agacctcctg ggcgcggccc ttcgccaacc tgaagatcgg cgtgcaggac
      541 acgatctaca cgcggttcaa cggcagcggg tccaactacg acgggttcgg gcgccgggcg
      601 ggggcgaaca acacgttgtt cgcctatgcc tggcttgcgt tctgatttcg gggagcggga
      661 ctcaggcgtg cagctgcgcg cgcgcgcgtt cggcaatatc gcgcgccaga tcggcgggca
      721 tctccccgac cacgacatag gcatacccgt cctgctgcca cgccgccacg cggagggcat
      781 cgtggcgttc aacgcgcagc ggccgtgacc caccttccat ctgactgacg cagagcgcca
      841 ccggaagccc ttgcgcgcgc gtatacatga gctgggcaac cggcctgccg tccaccacca
      901 gcatgcggcc gccggcaaag ctcagtccct cggcagaaag gtcggggatc tcgagatggc
      961 ggtggaggcg cttgtcgagc caggcgacaa actcctgcgc ctgcgcggcc ggcacctcga
     1021 cgagatggcc ccgttcccgc gagtagacgc tgtgatagtc cgcgatctcg tcgatcagtg
     1081 ccgccatggc ggactgccgc gtgcccagcc aagtgccgcc ggccccggcc gcgatgccaa
     1141 ggaccaggca ggccgcggct gcccagatga gacgccgacg cggccaggcg cgcggcaggg
     1201 cgggtggtag aagacgggcg gcatccgccg catagaccgt ctcgctacag gcggcacgca
     1261 acagtcccgc ggtttcccgg tacatctcca ccgtgcggcg cgcctggggg tcattggcga
     1321 gcaactgctc gacctccctc acgcccgcgg cgtcgagttc gccgtcgaca taggccagca
     1381 gcatcgtgtc ggttgccgtc atcgcccgca tcctacatcg accggaaggt ggagcggcca
     1441 ttcgggccgg gaaacaggct taccgacccg cttaaccgcg caccgagccg ttccgccagg
     1501 tcacgccgcg cccgcgacag gcggctcatc acggtgccga tcgcaacccc cagcacgtcc
     1561 gccgcctccc gatagctcag gccgtccacg caaaccagga tcaggaccgt acgctgttcg
     1621 gccggcatct cctgcagtcg gcggcgcacc agctccagcg tgatccgccc ctcggcaacc
     1681 gccggaccgt cgtcgccggc gacctccgtt gccacatcga gcggctcatg gcggcgccgc
     1741 gagcgcgtgc gcatctcgtc gatccagaga ttgcgcatga tgccgtacat ccacgcatcg
     1801 agccgggtct gatcccgcaa ttgcccctcc cgctgcaggg cgcgggcaca ggcgtcctgc
     1861 accagttcgt ccgcctccac ggccgagccg gtcagggcaa ggccgaaccg ccgcagccgc
     1921 ggcagcagcg ccgaaagcat cgggcgtagg tcctcggcca ctccgttctc ccgctctcag
     1981 ggaaatgacg cgccagcccg ccggttattc cctgcggcga aacagtgatc tctttccggc
     2041 cggggtgacc gaacggctgt gggtccgccg cttggatgct ccaagcctgc cgctgtcccg
     2101 cccgaatgca gggcaattac tgcacaccaa gcctgctgta ggcgttgtca acttaacgga
     2161 tggtgggcat tggccggcct tggagccggt cagcgtcaga cggtcactgg aacaccggcg
     2221 atatcgcgcc acgtgcggaa ggctgcatcg cgggtgctgc ggtattcgct ggcggacagg
     2281 cggtggcggc aaagctgaaa gtggttatgg atccggctgt gtgccgacag aaaacatcga
     2341 ccacaggaac tgcagacgat cccctgctcg tcatccccgc cgttcatcag cccgtcgctt
     2401 cagccgaatc gactgcctgt tgcggtgaga gagtgtcact ggcctccacg gcaacagttc
     2461 ggcccggagt ccttgccaga gcatcgagcg cctgccgcgt ccgacgcagc gcgaaatcga
     2521 ggtgtgcgag gcccgcttca aggtcactgt agtcgaagcc ctgcccctcc agcggggaaa
     2581 gcggcggcag tggcggcggg gcctccgcct ccggcgtgac cacgcggcgc ggcggccggc
     2641 cacggccgcg ccctggctcg ggtcggtacg ggggccgcac ggcgtcacgt agcggcgcca
     2701 tgccggtcaa cccgaacagc cgccgcttgg cgcggtgggt gacctcgacg gcgatcccgg
     2761 cggcagccag ttcatcgagc aggcggatcg cggtcttcac cgccacgccg agaatgccgg
     2821 caagcgaggt ggcggacagc agcggcacgg cggcaagcag gtcgaccgcg gcagcaacgt
     2881 gcgagtcccg gcgccggctg gccaccctgc gccgcgccat gatccagctg cattcaagat
     2941 ccgcgagcat ctgtcgggca tccgcggctt cttctgccag cgcgcggagg aagaacggcg
     3001 cccaggtcgc ggcgtcggcg ggcacgtcgg cacgcagggc ggccgcgccc gtcaacggta
     3061 ccggcaggcg cagcagc
//
LOCUS       DASSZH010000461         2312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_79224, whole genome shotgun sequence.
ACCESSION   DASSZH010000461 DASSZH010000000
VERSION     DASSZH010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79224"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VFN42_09990"
     rRNA            <1..302
                     /locus_tag="VFN42_09990"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            402..517
                     /gene="rrf"
                     /locus_tag="VFN42_09995"
     rRNA            402..517
                     /gene="rrf"
                     /locus_tag="VFN42_09995"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            550..626
                     /locus_tag="VFN42_10000"
     tRNA            550..626
                     /locus_tag="VFN42_10000"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:584..586,aa:Met,seq:cat)
     gene            836..1528
                     /locus_tag="VFN42_10005"
     CDS             836..1528
                     /locus_tag="VFN42_10005"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET8996987.1"
                     /translation="MRHRRSHSLAVIAAVAALLSAGAARSQSVQMFDEAPPLEVLRHI
                     MVPESSGGTSRSIVLGHPAPSEVQGPLQRVSTQARPMPHPAPPPSDSATAPLPAAAAA
                     QPMVQPATPAAGSDAAASAGVVGFHIRFAFDSVAIPADYAGFIQRIGTLLQQEPQVKL
                     RIEGHTDAVGTEEYNLNLSQRRALSVARYLVEQGGIAPERLAVVGKGKSEPITSNPYD
                     ADNRRVQFVRIE"
     gene            1658..>2312
                     /locus_tag="VFN42_10010"
     CDS             1658..>2312
                     /locus_tag="VFN42_10010"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HET8996988.1"
                     /translation="MLHRRRFGEAVFAILIVIAATAAALILLRPPSPLAAAERLFQAL
                     AFRLLAPTAPAAPRVVIIGITEATLAGFPYSAPIDRGFLAGLVDQLARAGVAAVGLDV
                     VFDRPTEPAKDAALRQALLRADAPVIAISLGPEAPEPPERRQFLRSFLDGALSGDANL
                     ARDRFDDMVRTHVPRDGATGQMSFPARIAAALGRAVPDHAFPILWQAQRPAVPTYPAE
                     "
BASE COUNT          370 a          824 c          766 g          352 t
ORIGIN      
        1 ttagaacgtc gtgagacagt tcggtcccta tctgccgtgg gtgaaggaga cttgcgaggc
       61 gctgtcccta gtacgagagg accgggatgg acgcacctct ggtgcaccgg ttgtcgcgcc
      121 agcggcaccg ccgggtagct atgtgcggac gggataaccg ctgaaagcat ctaagcggga
      181 aaccctcctc taaaccaggt ctccctgagg gccgtgcaag accagcacgt cgataggccg
      241 gatgtggaag tgcggcaacg catgaagctg accggtccta atcgcccgat aggctcaaga
      301 tccccaccac gcacagcagc gaaccacaca ctgtggcaac acagacggca cacacacact
      361 caccagaacc tgccacccac ccctcggggt gggccggacg acctggtggc tttggcgggg
      421 ggtgaaccac ccgatcccat cccgaactcg gccgtgaaaa cccccagcgc ccatggtact
      481 gcgacacaag tcgcgggaga gtcggtcgcc gccaggtcct cctgcccacc ccacccacac
      541 caccacccac gcggggtgga gcagcccggt agctcgtcag gctcataacc tgaaggccgc
      601 aggttcaaat cctgcccccg caaccacttt caccgaacac acgaacaaat caggaccacc
      661 agccaacgcg gctccctgcc gcagacgcgc ggccttctgg gcctgctgcg ccggtccttc
      721 cgcctgcgct gagttgggct gcgagtggcg tagctcacag tgccgtacca tggcctcctg
      781 catcgtaggc cggcgtcccg atggatgggc gctgccagca ggaaaggacc tcgccatgcg
      841 tcatcgccgc agccattccc tcgccgtgat cgccgctgtg gccgcgttgc tctcggccgg
      901 ggcggccaga tcgcagagcg tgcagatgtt cgacgaagcg ccgccgctgg aggtgctgcg
      961 gcacatcatg gtgccggagt cgtccggagg caccagccgc agcatcgtcc tcggccatcc
     1021 ggcaccgagc gaggtgcagg gtccgctcca gcgcgtgagt acccaagcgc ggcccatgcc
     1081 ccacccggcg ccgcccccct cagacagcgc aaccgccccg ctgccagccg ctgcggcggc
     1141 gcaacccatg gtgcaacccg cgacgccagc cgccgggtcg gatgccgccg cgtccgccgg
     1201 cgtcgttggc ttccacatca ggtttgcgtt cgattccgtc gcgatccccg ccgactacgc
     1261 cggcttcatc cagcggatcg gcacgctgct gcagcaggag ccgcaggtga agctgcgcat
     1321 cgagggtcat accgatgccg tgggaacgga ggaatacaac ctgaacctct cgcagcgccg
     1381 cgcgctgtcg gtcgcgcgct atctggtcga gcagggcggg atcgcgcccg agcggctggc
     1441 cgtggtcggc aagggcaagt ccgagccgat caccagcaat ccctatgacg cggacaaccg
     1501 gcgggtgcag ttcgtgcgga tcgagtagcg ggccgggcct gtggcgccgc agcattccgt
     1561 gagtagcatt ccgtgatcgg gggcggcgcc gcgccggctt gcgcttccgg catgcgctgc
     1621 cgagacttgg ccgacgacac accggtcggt cgtgaacatg ctccaccggc ggcgcttcgg
     1681 cgaggcggtg ttcgccatcc tcatcgtaat cgccgcgacg gcggctgcgc tgatcctgct
     1741 gcgtccgccc tcgccgctgg cggcggcgga gcgcctgttt caggcgctcg cattccgcct
     1801 gctggcaccg acggcgcccg ctgcgccgcg cgtcgtgatc atcggcatta ccgaggcgac
     1861 gctggccgga tttccctata gcgcgccgat cgaccgcggc ttccttgctg gtctggtgga
     1921 ccagttggcc cgcgccggcg tggcggcggt cgggctggac gtggtgttcg accggccgac
     1981 cgaaccggcg aaggatgcgg cgctgcgtca ggcgctgctt cgcgccgatg cccccgtgat
     2041 cgcgatctca ctgggacccg aggcgcccga gccaccggag cgccggcagt tcctgcggtc
     2101 ctttcttgat ggcgcactgt cgggcgacgc caacctcgcc cgcgaccggt tcgacgacat
     2161 ggtgcgcacc catgtgccga gggatggcgc gaccggccag atgagtttcc cggcgcggat
     2221 cgcggcggcg ctgggcaggg ccgtgccgga ccacgcgttc ccgatcctct ggcaggcgca
     2281 gcgtccggcg gtgccgacct atccggcgga aa
//
LOCUS       DASSZH010000462         3519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_982752, whole genome shotgun sequence.
ACCESSION   DASSZH010000462 DASSZH010000000
VERSION     DASSZH010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982752"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VFN42_10015"
     CDS             complement(<1..330)
                     /locus_tag="VFN42_10015"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET8996989.1"
                     /translation="MIEQTLDITTQDGEMETYICHPDRGGPHPVVFLLMDAPGIRDEL
                     RDMALRLGTVGYYVMLPNLYYRAGRDTIYGPDVLEHGSAEHQRMRAVRTRMTIPPVMD
                     DFAAMLRF"
     gene            655..1929
                     /locus_tag="VFN42_10020"
     CDS             655..1929
                     /locus_tag="VFN42_10020"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HET8996990.1"
                     /translation="MTSETYDGKVIHLGPDDSLIRKTELIISGVLRGGVLLSVAVILG
                     GIAWFYALRFAGALPHPTFPDTLPAVWQGLKMANPLAIVVLGLLILLATPVLRVAVSI
                     VAFALEEDRTYVIITALVLAILLFSIFGVGDWLGRPEPALVQDDTLVFFFLVLFSSAF
                     AGFIGALVGLGGGVFIVPILTLGFHVQFNAAIGASIVSVIATSSGAAAAYVRDRMTNL
                     RVGMFLEVATTLGAICGALLSTFLNATVLFIVFGVVLLISALPLVMRLGEELPANVIN
                     HKWAERLHLPGSYPDRRTHETVSYQVAHIRWGFSMMYVAGLVSGLLGIGSGTFKVLAM
                     DTAMRLPMKVSTTTSNFMIGVTAAASAGIFFQRGYIDPAIAAPVALGVLLGATVGART
                     LTRMSNVWLKRVFVPIIVLVALNMLARGMGWL"
     gene            complement(1975..2781)
                     /locus_tag="VFN42_10025"
     CDS             complement(1975..2781)
                     /locus_tag="VFN42_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET8996991.1"
                     /translation="MKAPFVLVLLPSTTGATECLTVATRVSETLGRAIQLAHVQVGPG
                     SIILPSQEQLSEYEAEHLAEREQAQTEAIRSLVATWSNSSGNQLPLDVFTGDEWRVMR
                     HYRGRVAMVVLASPHTQPVGHRETLRAALLRSGHPVVMVPPGWTGGLGRRLLVGWEDV
                     PPLRRSIEAFRPFLSAAEQVDVVAVEQTEASLDAAKALLEPIAPAARYRLVAAEGRRT
                     AAALLDEARTLQADGLVMGAFRRGEILNWLIPGTSSRLILESFLPLLMHP"
     gene            complement(2965..3462)
                     /locus_tag="VFN42_10030"
     CDS             complement(2965..3462)
                     /locus_tag="VFN42_10030"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET8996992.1"
                     /translation="MNAEELRAVQAPLKQRYRDTPEAARVPARAEARLDTAEVACTIP
                     SWQGETTAGLHPATGGDGALACSADMLLQAVVACAGVTLRAVATAMGVTIRSGKVIAE
                     GQWDARGTLGIDRSAPVGLTDIRLRFEVDSDADPAKLERMIQTTERYCVILQTLKNPP
                     HISVG"
BASE COUNT          535 a         1127 c         1221 g          636 t
ORIGIN      
        1 gaagcgcagc atggcggcga agtcgtccat caccggcggg atcgtcatcc gggtgcgcac
       61 cgcccgcatc cgctggtgtt ccgcgctgcc gtgttccagc acgtccggcc cgtagatcgt
      121 gtcccggccc gcacgatagt acaggttcgg cagcatcaca tagtagccga ccgtgccaag
      181 ccgcagcgcc atgtcccgca gctcgtcgcg aatgccgggg gcgtccatca gcaggaacac
      241 cacgggatgc gggccgccac ggtcggggtg gcagatatag gtttccatct ccccgtcctg
      301 ggtggtgata tccagggtct gctcgatcat gtggcggtcc cctccgtggg cgttgcggcc
      361 aggtcacgat cccatgcggg atgcagtgcg gcaagccggc cgccgcgagc aggcaggcca
      421 tcgccgcgcc tcgcagggtg gcggcgacgg gttggtgtgc ggcgtgagcg ggcgcgccgc
      481 acctggcttc gggagcggtc gcgtggccgt gcggccacaa aatcattaat tgtcggacac
      541 aagctggcct ggcgcgaatc aggccataat ttcttctgcg ctgatgaaat atcaagccgc
      601 ctcgacgagc agaatcccct gcccgccata ccgacgattc tgtttgcagg cacgatgacg
      661 agcgaaacct acgacggcaa ggtcatccac cttgggccgg acgacagtct aatccgcaaa
      721 accgaactga tcatcagcgg cgtgctgcgc ggcggcgtgc tgctcagcgt ggcggtgatc
      781 ctgggaggta ttgcctggtt ctacgcgctg cgctttgccg gcgcgctgcc ccatccgaca
      841 tttccggaca cgttgccggc ggtgtggcag ggattgaaga tggccaatcc actggccatc
      901 gtggtgcttg gattgctgat cctgctggca acaccggtcc tgcgcgtcgc ggtttccatc
      961 gtcgcgtttg cgctggagga ggaccggacc tacgtcatca tcacggcgct ggttctggcg
     1021 atattgttgt tcagcatttt cggcgttggc gactggctcg gtcggccaga gccggcgctg
     1081 gtgcaggacg acacgctggt cttcttcttt ctggtactgt tcagctcggc attcgccggg
     1141 ttcatcggcg cgctggttgg gctgggtggc ggcgtgttca tcgtgcccat cctgacgctg
     1201 ggcttccatg tgcagttcaa cgcggcgatc ggcgcgtcca tcgtctcggt gatcgccacc
     1261 tcgtcgggtg ccgcggccgc ctacgtgcgg gatcgcatga cgaacctgcg ggtcggcatg
     1321 ttcctggaag tggcgaccac cttgggtgcg atctgcggtg cgctgctgtc cacgttcctg
     1381 aacgcgaccg tgttgttcat cgtgttcggc gtcgtgcttc tgatctctgc cctgccgctg
     1441 gtgatgcgac ttggcgagga actgccggcc aacgtcatca accacaaatg ggccgagcgg
     1501 ctgcacttgc cggggagcta tcccgaccgg cggacacatg agaccgtgtc ctatcaagtg
     1561 gcgcatatcc gctggggctt cagcatgatg tatgtggccg ggctggtctc cgggctgctg
     1621 gggatcggca gcggcacgtt caaagtgctg gcgatggaca cggcgatgcg cctgccgatg
     1681 aaggtctcca ccacgaccag caatttcatg atcggcgtga ccgcggcggc ttcggccggg
     1741 atcttcttcc agcgcggcta catcgatccg gccatcgccg cgccggtcgc acttggggtg
     1801 ctgctggggg ccacggtcgg cgccaggaca ctgacccgga tgtccaatgt gtggttgaag
     1861 cgggtgttcg tgccgatcat cgtgctggtg gcgctgaaca tgctggcgcg tggcatgggc
     1921 tggctgtagg cagcggctgc ggtgtggcgg gcgcgccccg ccacaccgga gacttcaggg
     1981 gtgcatcagc agcggcagga agctttccag gatcagccgg ctggatgtgc ccggaatcag
     2041 ccagttcaaa atctcgccac gccggaacgc gcccattacc aggccatcgg cctgaagcgt
     2101 ccgcgcttca tcgagcaagg ccgcggccgt ccggcgtcct tccgcggcga ccagccgata
     2161 gcgagccgcg ggggcgatcg gctccagcag cgccttggcg gcgtccagtg aagcctcggt
     2221 ctgctcgacg gccaccacat cgacctgctc cgccgcggag aggaacggcc tgaaagcctc
     2281 gatcgatcgg cgcagcggcg gcacgtcttc ccagccaacc aggagccgcc ggccgaggcc
     2341 gccggtccag ccgggcggga ccatcaccac cgggtgtccg gagcgcagca acgctgcccg
     2401 cagcgtttcg cgatggccga cgggttgggt gtggggagag gcgagtacca ccatcgccac
     2461 cctgccgcgg taatggcgca ttacccgcca ctcgtctccg gtgaagacgt ccagcggcaa
     2521 ctggttcccg gagctgttgc tccaggtggc gaccagcgag cggatggctt ccgtctgggc
     2581 ctgctcacgt tccgcaaggt gctcggcttc gtattccgag agctgctcct gggacggcag
     2641 aatgatggaa ccgggcccga cctgcacgtg ggcaagctgg atagcgcggc caagcgtttc
     2701 actgaccctg gtcgcgactg tcaggcattc cgtcgcccct gtggtggagg gcaatagaac
     2761 caggacaaag ggtgctttca tggggcagag actagcgacc gcgcgcgagc aaatgcaaac
     2821 atgctgcaag catttgtgtc acggccggcg ggaattggct gcgccttgcc atctgtcacg
     2881 gccagcccag gcgggtcccc aggatgctgc ggcgtaatcc ggcggacagc cgcgcaggcg
     2941 ggccggctaa actgcgggcg gcggtcagcc gaccgaaatg tgcggcgggt tcttcagcgt
     3001 ctgcaggatg acgcagtagc gctcggtggt ctggatcatg cgttccagtt tcgccgggtc
     3061 ggcgtcgctg tccacctcga agcgcaggcg aatgtcggtc aggccgacgg gcgcgctgcg
     3121 gtcgatgccc agcgtgccgc gggcatccca ctggccctcg gcgatcacct tgccgctgcg
     3181 gatggtcacg cccatggcgg tggcaaccgc ccgcagagtc acgccggcgc aggccaccac
     3241 cgcttgcagc agcatgtcgg cggagcaggc gagggcgcca tccccgccgg tggccggatg
     3301 caggccggcc gtggtctcgc cctgccagga ggggatggtg caggccactt cggcggtgtc
     3361 cagccgagcc tcagctcggg cagggacccg tgcggcctcc ggggtgtcgc gatagcgctg
     3421 cttcagcggc gcctggacgg cgcgcagttc ttcggcgttc atggcagacc tccctttccc
     3481 gtgacgcggc gtggtgccgc cgggcggatc aggccgcgg
//
LOCUS       DASSZH010000463         2670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1340564, whole genome shotgun sequence.
ACCESSION   DASSZH010000463 DASSZH010000000
VERSION     DASSZH010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340564"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1710
                     /gene="glgP"
                     /locus_tag="VFN42_10035"
     CDS             <1..1710
                     /gene="glgP"
                     /locus_tag="VFN42_10035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HET8996993.1"
                     /translation="TVFHLNEGHAAFAVLERARSLSVRLGIGFEEALWASRAGNVFTT
                     HTPVSAGFDLYPAGLLEQYRRVIGGQIAESIAATAVALSGATRETGFNMAYLALRGAY
                     ASFGVSALHGAVSRHIFQGLFPRWPEQEVPVAHVTNGVHVPSWESPAADAVFSGNGKD
                     PWQRAPETVRATLAQADDATLWEMRAHSRQHLVLSVRERLHKHLTGRGQAASVVALAE
                     NVLDPNVLTLGFARRFTAYKRPNLLLRDRPRLERLLGDSHRPVQLVVAGKAHPADEEG
                     KRMIAEWVAFTAHPDLWHRVVFLEDYDIALAQEMVQGVDVWINTPRRPWEACGTSGMK
                     VLANGGINLSELDGWWAEAYAADLGWAIGTTRQPDDATQDARDAEELYTLLEQVVLPE
                     FYTREAGIPRAWIARVRQSIAALAPRFSATRMVQDYLTQGYLPAAAALRRRLADGAAV
                     AHDLRQWERRLHRGWRHPHIGTPEISREADGWSWALSVYLGEIGPDDIRVELYADATA
                     TAPAQTVALTRGTPITGSVGGFFYTGRVATTRPPEDYTPRIVPFHAEARVPAELPLIF
                     WKE"
     gene            1716..>2670
                     /locus_tag="VFN42_10040"
     CDS             1716..>2670
                     /locus_tag="VFN42_10040"
                     /inference="COORDINATES: protein motif:HMM:NF028488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET8996994.1"
                     /translation="MARAAEAIGAKLLLDRDGLQSLLDELHARGFDVVGPTVRDGAIV
                     HDRISRTDEMPAGWGDRQEAGHYRLERRADAALFGFNSGPHAWKQFLHPPEERLFRAT
                     RQGEGFALQPEPPPAAPFAFIGVRACDLHAIAIQDRVFLGGSHPDRCYAGRRHGAFIV
                     ALNCGTAGATCFCASMHTGPHATAGFDIALTELIDDARHDFLAEVGSETGADVLAALH
                     SRPAGPQDTQAAASVLARTEAAMGRRMDTDGLKQRLQDNPEHPRWDDVAARCLSCGNC
                     TMVCPTCFCTSVADHTDLTGTEAERVKRWDSCFTADFSYIHG"
BASE COUNT          397 a          928 c          946 g          399 t
ORIGIN      
        1 accgtcttcc acctgaacga gggccacgcc gccttcgccg tactggaacg ggcacgcagc
       61 ctctccgtgc ggctcggcat cgggttcgag gaggcgctgt gggccagccg cgccggcaac
      121 gtgtttacca cgcatacgcc ggtcagcgcc gggttcgacc tttatcccgc aggcctgctg
      181 gaacagtatc ggcgtgtcat cggcggccag atcgcggaga gcattgccgc aaccgccgtg
      241 gcgctgagcg gtgccacgcg cgagacgggc ttcaacatgg cctatctcgc cctgcgcggc
      301 gcctatgcca gtttcggcgt cagtgcgctg cacggcgccg tcagccggca catcttccag
      361 ggcctgttcc cgcgctggcc ggagcaggag gtgccggtgg cgcacgtgac caacggggtg
      421 catgtgccgt cctgggaatc accggcggcg gacgcggtgt tctccggcaa cggcaaggat
      481 ccgtggcagc gggcgccgga gacggtgcgc gccacgctcg cacaggcgga cgacgcgacg
      541 ctgtgggaga tgcgcgccca ctcccgccag catctggttc tgtcggtgcg cgagcggctg
      601 cacaagcacc tcaccggccg cggccaggcc gcatcggtgg tcgccctggc cgagaacgtg
      661 ctcgatccga acgtgctgac gctcggcttt gcgcgtcgct tcaccgccta caagcggccg
      721 aacctgctgc tgcgggaccg gccccggctg gagcggctgc tgggcgattc gcatcgaccg
      781 gtgcagcttg tcgtggccgg caaggcgcat cccgccgatg aggagggcaa gcgcatgatc
      841 gcggaatggg tcgccttcac cgcccacccg gatctgtggc accgcgtcgt gttcctggag
      901 gattacgaca tcgcgctggc gcaggagatg gtgcagggcg tcgatgtctg gatcaacacg
      961 ccgcgccggc cgtgggaggc gtgcggcacc agcggcatga aggtgctggc gaacggcggc
     1021 atcaacctgt cggaactcga cgggtggtgg gcggaggcgt acgcggccga tctcggctgg
     1081 gcgatcggca ccacgcggca gccggacgac gccacgcagg atgcgcgcga cgccgaggaa
     1141 ctctacacgc tgctggaaca ggtggtgctg ccggagttct acacccgcga ggccggcatt
     1201 ccgcgggcct ggatcgcgcg ggtgcggcag agcatcgccg cgctggcacc gcgcttcagc
     1261 gccacgcgca tggtgcagga ttacctgacc caaggctatc tgccggccgc cgccgcgctg
     1321 cggcgacgcc tcgccgacgg ggcggcggtg gcgcatgacc tgcggcaatg ggaacgccgg
     1381 ctgcatcgcg gctggcgcca cccgcatatc ggcacgccgg agatcagccg cgaggcggat
     1441 ggctggtcct gggcgctgtc ggtctatctt ggcgagatcg gtccggacga cattcgcgtc
     1501 gaactctatg ccgacgccac cgccactgcg ccggcgcaga cggtcgcgct gacccgcggc
     1561 acgccgatca ccggttcggt gggaggattt ttctacaccg gccgggtcgc cacgactcgt
     1621 ccacctgagg actacacgcc gcgcatcgtg ccgttccacg cagaggcgcg cgtgccggcc
     1681 gaactgccgc tgatcttctg gaaggaatag cgggcatggc gcgagcggcg gaggcaatcg
     1741 gcgcgaaact tctgctcgac cgggacgggc tgcagtcgct gctcgatgaa cttcatgcac
     1801 gcggcttcga cgtcgtgggg ccgaccgtgc gggacggcgc catcgtgcat gaccggatca
     1861 gccggaccga cgagatgccg gccggctggg gcgaccgcca ggaggccgga cactaccgac
     1921 tggaacggcg cgccgatgcg gcgctgttcg gtttcaattc cggcccgcac gcctggaagc
     1981 agttcctgca tccgccggag gaacgcctgt ttcgcgcgac gcggcagggc gagggattcg
     2041 cgctgcagcc ggaaccgccg ccggccgccc cgttcgcgtt catcggcgtg cgtgcctgcg
     2101 acctgcatgc aatcgccatc caggaccgcg tgttcctcgg cggatcgcac ccggaccgct
     2161 gctatgccgg gcggcggcat ggcgccttca tcgtcgcgct gaactgcggc acggcgggcg
     2221 caacctgttt ctgcgcctcg atgcacaccg gcccgcacgc gacggccggc ttcgacatcg
     2281 cgctcaccga gctgatcgac gacgcgcggc acgacttcct cgccgaggtc ggcagcgaga
     2341 cgggcgccga cgtgctcgcc gcgctgcact cacgcccggc cggaccgcag gacacgcagg
     2401 cggcggcgtc cgtcctcgcc cgcaccgagg ccgcgatggg ccgccggatg gacaccgacg
     2461 gcctgaagca gcggttgcag gacaatcccg agcatccgcg ctgggacgat gtggccgcac
     2521 gctgcctttc ctgcggcaac tgcaccatgg tctgcccgac ctgtttctgc acctccgttg
     2581 ccgaccatac cgacctgacc gggaccgagg cggaacgggt gaagcggtgg gattcctgct
     2641 tcaccgccga tttctcgtac atccacgggg
//
LOCUS       DASSZH010000464         3132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1511327, whole genome shotgun sequence.
ACCESSION   DASSZH010000464 DASSZH010000000
VERSION     DASSZH010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511327"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VFN42_10045"
     CDS             complement(<1..540)
                     /locus_tag="VFN42_10045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8996995.1"
                     /translation="MLPIAVVIPTLNAAATLAGTCAALDGVAEIVVSDGGSSDGTRRL
                     ARRLGCRVVSAPRGRGGQLMAGIAAARQEWLLLLHADTRLQPGWQAAVSGHMQAGPDK
                     AGYARFALDSSDRRARRLERLVAWRCRVLALPYGDQGLLVHRALLAEVGGIRPLPLME
                     DVDLVRRLGRRRLAGLDATA"
     gene            616..1797
                     /locus_tag="VFN42_10050"
     CDS             616..1797
                     /locus_tag="VFN42_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HET8996996.1"
                     /translation="MRENPQDSKDMEALAALEQAGAPEPQATYSRPPLARQGRGATTN
                     PSVRFDRFSASPFDDGWDTLDADFAALPKLETTLTRDSSKSAISWNSSPDIGFDRAVN
                     PYRGCEHGCIYCYARPTHAYLGYSPGLDFETRLIFKPEVAELLEKELRKPGYVPRCLA
                     LGSNTDPYQPVERTLKLTRSVLQVLDRFNHPVSIVTKSAGVLRDLDILSGMAKRGLAR
                     VYVSVTTLDARLARVMEPRAATPARRLQTVAELTRAGVPVGVMAAPMIPGLNDAELEK
                     ILEAAARAGARHGAYILLRLPHELRQMFQDWLSTHFPDRAKHVLSLIRETRAGALSDA
                     RFHHRFAGQGVYADLLLRRFTRAARQWGLDEAREGLDCTKFAVPDAGAAANAPAQMSL
                     F"
     gene            1997..2566
                     /locus_tag="VFN42_10055"
     CDS             1997..2566
                     /locus_tag="VFN42_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3576 domain-containing protein"
                     /protein_id="HET8996997.1"
                     /translation="MPIRWFGISLLLTASLGLAACSGVQTEAPTLNRCDPGTDCGSLT
                     GSSQRLQSSEMQAAPSQDNRSGFSAIFGFGGQSTQNNGGNSLAVNAFLWRGALDTISF
                     MPLASADPFGGVILTDWYTPPSSPNERFKATIFILGRELRSNNLRISIFRQVLQNGQW
                     VDAQVSPNTVNDIEDKVLARARRLRVEMK"
BASE COUNT          508 a         1072 c         1070 g          482 t
ORIGIN      
        1 cgcggtggca tccaagccgg ccagccgccg ccggccaagc cgccggacga gatcgacatc
       61 ctccatcagc ggcagcggcc ggataccgcc gacctcggca agcagggcgc ggtgcaccag
      121 caggccctgg tcgccgtagg gcagcgccag cacccggcag cgccaggcga ccaggcgttc
      181 cagccggcgg gcgcgccggt cgctgctgtc cagggcgaag cgggcatagc cggctttgtc
      241 cggcccggcc tgcatgtggc cggacaccgc cgcttgccag cccggttgca gccgggtgtc
      301 ggcgtgcagc agcagcagcc attcctgccg agccgcggcg atcccggcca taagctgccc
      361 gccgcggccg cgcggagcgg acaccacgcg gcagcccagg cgccgggcga ggcgccgggt
      421 gccatcgctg ctgccgccgt cgctcaccac gatctccgcc accccgtcca aggcggcgca
      481 agtgcccgcc agggtggccg cggcgttcag cgtcgggatc acaaccgcga tcggaagcac
      541 cggatattct ctttctgttc ttgaccgttg cgtggggctc tgccaaggtg tcgaggaacg
      601 gactgggaac gcacgatgcg ggaaaatcct caggacagca aggacatgga ggcgctcgcc
      661 gcactggagc aggccggcgc gccggagccc caggcaacgt acagcaggcc gccgctggcc
      721 cggcagggcc ggggtgcgac gaccaatcca tccgtccgct tcgaccggtt cagcgccagc
      781 ccgtttgatg acggctggga cacgctggac gcggatttcg cggcgctgcc gaagctggaa
      841 accacactga cgcgggattc cagcaaatcg gccatcagtt ggaacagctc gccggatatc
      901 ggcttcgacc gtgcggtgaa cccgtatcgc ggctgcgagc acggctgcat ctactgctac
      961 gctcggccga cgcacgccta tctgggatat tcgcccgggc tggatttcga gacgcggctg
     1021 atcttcaagc cggaggtcgc ggagctgctg gaaaaggagc tgcgcaagcc gggctatgtg
     1081 ccgcgctgtc tggcgctggg gtccaacacc gatccctacc agccggtgga gcgcacgctg
     1141 aagctgacgc gcagcgtgtt gcaggtgctg gaccggttca atcatccggt cagcatcgtc
     1201 accaagtcag ccggggtgct gcgtgacctg gacattctct ccggcatggc gaagcgcggt
     1261 ctggcgcggg tgtatgtgtc ggtcaccacg ctggatgccc ggcttgcccg ggtgatggaa
     1321 ccccgcgcgg cgacgccggc gcgccggctg cagaccgtcg cggagctgac ccgggccggc
     1381 gtgccggtgg gcgtgatggc ggcgccgatg atccccgggc tgaacgacgc cgaactggag
     1441 aagatccttg aagccgcggc acgcgccggt gcccggcacg gcgcctatat cctgctgcgc
     1501 ctgccgcatg agctgcggca gatgttccag gactggctga gcacgcattt tccggaccgt
     1561 gccaagcacg tgctcagcct gatccgcgaa acccgtgccg gggcgctgag cgatgcgcgg
     1621 ttccaccacc gcttcgccgg ccagggtgtg tacgccgatc tcctgctgcg ccgcttcacg
     1681 cgtgccgcgc gccaatgggg tctggatgag gcgcgtgagg gactcgactg cacgaagttt
     1741 gctgtgccgg atgccggcgc ggcggcaaat gcaccggcac agatgtcgct gttctgatgc
     1801 tgttgcggag acccgatcgg ccggctgcgg cgtggccgcc ggccgccggg cgccgaatcg
     1861 gccggcctgc atgcgccgcg tcgcagccgg cggagactgc cagcctgcac ggcggcggac
     1921 tggcattgcg gttggatttg gcggccggcg gcaaatcccc taagtagcgg caccgaaaat
     1981 cgctcaaggt gtcgtgatgc ccatccgctg gttcggaatc tccctgctgc tgacggcctc
     2041 gctggggctc gctgcctgct ccggcgtgca aaccgaggcg cccacgctca accgctgcga
     2101 tcccggtacg gattgcggca gcctgaccgg ctcctcgcag cgtctgcaat ccagcgagat
     2161 gcaggcggcg ccctcacagg acaaccgcag cggcttcagt gcgatcttcg gctttggcgg
     2221 ccagagcacg cagaacaacg gcggcaattc gctggcggtc aatgcgtttc tctggcgcgg
     2281 cgcattggac accatcagct tcatgccgct ggcttcggcc gatccgttcg gcggggtgat
     2341 cctcaccgac tggtacacgc cccccagctc acccaacgag cggttcaagg ccaccatctt
     2401 catcctgggc cgcgagcttc gcagcaacaa cctgcggatc agcatcttcc gccaggtgct
     2461 gcagaatggg cagtgggtgg atgcccaggt cagcccgaac acggtgaacg atatcgagga
     2521 caaggtgctc gcccgcgccc ggcggctgcg cgtcgagatg aagtgaatca gccgggttgc
     2581 aaatccgggg acgagaacgc tgccgcgaat gcggcgccat actgagccgc gttggcaacc
     2641 ggaacgcggc cgcgccgtga ccggcgaagg tcacggcacg gggggtaaac gcaccagccc
     2701 gcagtgatgt ccgcgttgcg tcatgccggc atccagggct tgccggcgac acgccgaata
     2761 tggcgggccc cgccacgacc gggtcctgcc gccggcgtgt catcaccagg gcgtggtgtc
     2821 ataccaaccc gcgatgagct cgactcttcg gtacgccgaa ttccgtcatg ccggcgaaag
     2881 ccggcatcca cgactttcct cagtcgcacg cagcgacgtt gtgggtggcg agcctgcgtc
     2941 cgccacgacg tgataccgcg gccgcgagtc gaattcgccg ccggtgggta tcagatgacc
     3001 cccgccgcac gaaaatcact gatctccgct gcggcatagc ccagatcctc cagcacctcc
     3061 tcattgtgct gcccgaatat cggcggcggg gtgtcgattc gcggaccgtc atgcgcgaac
     3121 ttgaaggccg cc
//
LOCUS       DASSZH010000465         2300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1408605, whole genome shotgun sequence.
ACCESSION   DASSZH010000465 DASSZH010000000
VERSION     DASSZH010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408605"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VFN42_10060"
     CDS             complement(<1..362)
                     /locus_tag="VFN42_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8996998.1"
                     /translation="MKIVVIGGSGLIGSNLVNRLRLAGHDVLAASPRSGVNTITREGL
                     AEALSGAQVVVDVANAPSFEDAPVLEFFQTSGRNLLAAETAAGVAHHVALSIVGTDRN
                     TDSGYMRAKLAQEGLIKWS"
     gene            573..2150
                     /locus_tag="VFN42_10065"
     CDS             573..2150
                     /locus_tag="VFN42_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET8996999.1"
                     /translation="MQFLFAGYLLDPDRRELTRGTEVIAVGPQVFDLLLHLLRNREHV
                     VSKDELLDAVWSGRIVSESTLASHINAARKAIGDTGEEQRLIRTIARKGFRFVAEVSE
                     AQPQEAVPASEPAASEEMPPPALHLPDKPSVAVLPFQNMSGDPEQEYFADGVVEDIIT
                     GLSRIRWLFVIARNSSFTYRNRMVDVKQVGRELGVRYVLEGSVRKAGNRVRITGQLVD
                     ATTGAHLWAERFDGALDDIFELQDQIATSVVGAMGPRLERAEIERAQRMPTPSLNAYD
                     CYLRGIAHMHQGNREATEAALPLFYHAMELDPDFASAAAMAAWCFLWRKANGWMTDPA
                     YEAAEGARLARIAVETGREDAVALTRAGSAFAHFVGDFDRGVALLDRAVVLNPSLAAA
                     WFLGGFLRLWLGEADDAIAHFVRGMRLSPLDPEMYRMQAGMAAAHLFAGRFDAASSWA
                     DKAFTNLPSFLMAVSVIAASHALAERPEQARRAMAHLRRLDPTWRISQIADRFPIRRP
                     EDRAIFTDGLRKAGLPE"
BASE COUNT          382 a          765 c          769 g          384 t
ORIGIN      
        1 gaccacttga tcagcccttc ctgcgccagc ttcgcgcgca tatagccgct gtcggtattg
       61 cggtcggtgc cgacgatcga caacgcgaca tgatgcgcca cgccggcggc ggtttcggcg
      121 gccagcaggt tgcggccgga ggtctggaaa aactccagga caggcgcgtc ctcaaaggag
      181 ggcgcgttcg ccacgtccac caccacctgc gcgccggaca gcgcttcggc cagaccctca
      241 cgggtgatgg tgttgacgcc cgaacgcggc gaagccgcga gcacgtcatg ccccgccaaa
      301 cgcaggcggt tgacgaggtt cgagccgatg aggccactgc ctccgatgac cacgatcttc
      361 atcctgttct cctctgtccc actgacgatc acgcaaccgc gatctcagtg agaagaggat
      421 gccagcacgc ccggcaaaag actttgccgt gggcttggat ggagcttgga attcccttgg
      481 ttatccgtcc aagcggcgat gcagtgcccc ggcgatgcct ggcatgctat cagtggctgg
      541 cccgccgccc ggcggggaca cggagtgcca aggtgcaatt cctgttcgca ggctatctgc
      601 tcgatcccga ccggcgcgag ttgacgcggg gcaccgaggt gattgccgtc gggccgcagg
      661 tattcgacct gctgctgcat ctgctgcgca accgcgaaca cgtcgtctcc aaggatgagc
      721 tgctggacgc ggtatggagt gggcggatcg tctcggaatc gacactcgcc agccacatca
      781 acgccgcgcg caaagccatc ggcgataccg gcgaggaaca gcggctgatc cgtaccatcg
      841 cccgcaaggg cttccgcttc gtcgccgaag tcagcgaagc acagccgcag gaagctgtcc
      901 cggcttccga accagccgca tcggaggaaa tgccgccacc ggcgctgcat cttcccgaca
      961 agccctccgt cgccgtcctg ccgttccaga acatgagcgg cgatccggag caggaatact
     1021 tcgccgatgg cgtggtcgag gacatcatca ccggcctgtc gcgcatccgc tggctgtttg
     1081 tcatcgcgcg caactccagc ttcacctacc ggaaccggat ggtggacgtg aagcaggtgg
     1141 gccgcgaact cggcgtccgc tatgtgctgg aaggcagcgt gcgcaaggcc ggcaaccgcg
     1201 tgcgcatcac cggtcagctg gtcgatgcga cgaccggcgc gcatctgtgg gcggagcgct
     1261 tcgacggcgc gctggatgac atcttcgaac tgcaggacca gatcgccacc agcgtcgtcg
     1321 gtgcgatggg tccgcggctg gagcgcgcgg agattgagcg cgcgcagcgt atgccgaccc
     1381 cgagtctcaa tgcctatgac tgctatctcc gcggtatcgc gcatatgcac cagggcaacc
     1441 gggaggccac ggaggcggcg cttccgctgt tctatcacgc gatggaactc gatccggact
     1501 tcgcatccgc ggcggcaatg gcggcgtggt gctttctctg gcgcaaggcc aatggctgga
     1561 tgaccgatcc ggcgtatgag gccgcggaag gtgcccggct ggcccggatc gcggtggaaa
     1621 caggcaggga agatgcggtt gcgctgaccc gcgccggcag tgcgttcgcc cacttcgtcg
     1681 gcgacttcga ccgtggggtt gccttgctcg accgcgcggt ggtgctcaat ccaagcctgg
     1741 cggcggcatg gttcctgggc gggttcctgc ggctttggct cggcgaggcg gacgacgcca
     1801 tcgcgcattt cgttcgtggg atgcggctga gcccgctgga tccggagatg taccgcatgc
     1861 aggcgggcat ggcggcggcg cacttgttcg ccggacgctt cgacgccgcg tcgtcctggg
     1921 cggacaaagc attcaccaac ctgcccagct tcctgatggc ggtcagcgtg atcgccgcga
     1981 gccacgcgtt ggccgagcgg cccgaacagg cgcggcgggc catggcgcat ctgcgccggc
     2041 tcgatcccac gtggcgcatc tcccagatcg cggatcggtt cccgatccga cggccggaag
     2101 atcgtgcgat tttcacggat ggcctgcgca aggcggggct gccggaatga ccggcggccc
     2161 aaaccggacg cggggcgccg cgtcagggct ggaaccaccc ggctgcgcct gtggcgcacc
     2221 agccgcgctc ccgtgcgact tggtcaatcc gcccctgccc ggctaaatgc ccctatgccg
     2281 accttcacac cgcaggccct
//
LOCUS       DASSZH010000466         3870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_539660, whole genome shotgun sequence.
ACCESSION   DASSZH010000466 DASSZH010000000
VERSION     DASSZH010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539660"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VFN42_10070"
     CDS             complement(<1..588)
                     /locus_tag="VFN42_10070"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET8997000.1"
                     /translation="MLDADPQLQVAAVASAATSHTTTCDGCRIAWKQWGAGPPLVLLH
                     GDAGSWTHWIRNVLALGRRFRVLVPDLPGYGASATPGQPATPASLAALLAEGLAALPD
                     APRRYRLAGFSFGGIVAGHLAALDVDRVERLILLGAGGLGLPIPPLSAPLRRPGRDMM
                     AAELADVHRHNLAVLMFGDPARVDRLATWLQIENVR"
     gene            840..1700
                     /locus_tag="VFN42_10075"
     CDS             840..1700
                     /locus_tag="VFN42_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET8997001.1"
                     /translation="MESDAAAASFAALSQPTRLDLIRLLIAQGANGLPAGEIASRLGL
                     PASTLSFHLAALERAGLTQSTRQGRQIIHAARIAGLRQLLGFLTETCCGGRPELCGDI
                     HRLLPPLPDEAPGLMPAFNVLFLCTHNSARSIMAEAILEQVGAGRFRAYSAGSDPIPA
                     PLPEVLEKLRAFGHDVSGLHSKSWDAFLGPSAPAMDFVISLCDTPEGQVCPDFSEVAV
                     TAAWPLPDPAKFTGSAVERASMLNELYASLRRRMEIFTALPFASLDRMAIRARLDEIG
                     GGLRPPLGAR"
     gene            1703..2746
                     /locus_tag="VFN42_10080"
     CDS             1703..2746
                     /locus_tag="VFN42_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsJ-associated glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HET8997002.1"
                     /translation="MRVGINGMGRIGRLALRAALGRIYRPADDPRADNRLDVVHVNEL
                     RGGAAATAHLLEFDSVHGRWRDSFGVEGDHAIHIGNRRIGFSAEPEAGGVAWGELGCD
                     LVLECTGKFLKQEQLAGYFARGVKRVIVAAPVKDGSALNIVVGVNDDRYDPREHRLLT
                     AASCTTNCLAPVVKVLHEAVGIRHGQITTIHDPTNTNVVIDAPHHDLRRARSAMLSLS
                     PTTTGSATAIALIYPELKGRLNGHAVRAPVLNASLTDCVFELMRPTDVDAVNGLFRAA
                     AAGPLAGILGFETRPLVSADYANDPRSAIIDAPSTMVTDGTLVKVYAWYDNEVGYACR
                     MVDLANIVMQRGI"
     gene            2746..>3870
                     /gene="arsJ"
                     /locus_tag="VFN42_10085"
     CDS             2746..>3870
                     /gene="arsJ"
                     /locus_tag="VFN42_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organoarsenical effux MFS transporter ArsJ"
                     /protein_id="HET8997003.1"
                     /translation="MSSGLRNYAIVTTAYFLFTITDGALRMLVLLHFYSLGYTPFTLA
                     LMFLLYETAGIFANAGGGWMASRYGIPRMLMAGQMLQIGGLCMLSALQPGWSAALSVA
                     WVVLAQGVAGVAKDFTKTSSKSAIKASATQDMGQLFRWVAWFTGSKNATKGAGFFIGG
                     FLLQVAGFQPALWLMAAMLALVLAGVALSLPRTLGKAKSSKSFRELFAKSRAVNLIAA
                     ARIFMFGSRDVWFVVALPVYLYGFGWQYMQVAGFVAAWTIAYGAVQALAPQVVTRSRD
                     GLSREVPAARLWGAILTVIPVALALLLTMPGVPRPDLILVVGLLVFGFVFAVISSLHS
                     YLILAYAGSEKAAEDVGFYYAANAAGRLMGIMLSGLLTETG"
BASE COUNT          580 a         1344 c         1289 g          657 t
ORIGIN      
        1 gcggacattc tcgatttgca accaggtggc gagtcggtcg acgcgagccg ggtcgccgaa
       61 catcagcacc gccaggttgt gccggtgcac atcggcgagc tcggcggcca tcatgtcgcg
      121 cccaggccgg cgtagcgggg ccgatagcgg cggtatcggc aagccaagac cgccggcgcc
      181 cagcaggatc agccgctcga cccggtccac gtccaatgcg gcgaggtggc cggcaacgat
      241 cccgccaaag gaaaagccag ccaggcgata gcggcgcggg gcgtccggca gggcagcaag
      301 cccctccgcc agtaaagcgg caaggcttgc cggcgtcgcc ggctggccgg gagtggccga
      361 agccccgtaa ccgggcaggt ccggcacgag cacacggaag cgccgcccga gggccaggac
      421 attgcggatc cagtgggtcc acgatccggc atcgccgtgc agcagcacca acggagggcc
      481 ggcgccccac tgcttccatg cgatgcgaca cccgtcgcag gtggttgtgt ggctcgtggc
      541 cgcgctggcc accgcagcga cctgaagttg cggatcggcg tcaagcatgg ggccctcaac
      601 cagagtcgcg gaaccggcac ggcaagcagc ctggcctgct tgccacgtca tccacgacgg
      661 gcgtcaccac ggctcgccct ggcgttgcga cggatgccat caccgctgat gcactgtcca
      721 ccgcatgcgg cctgctgccg cggcagcgtg ccgccgaaat cgccgaaggc cttgctgcgc
      781 ggccgctgtg gcgtggttgc ccagttgcag ccggaagaaa tttccattat tctggaacca
      841 tggaatcgga tgccgccgcc gccagcttcg ccgccctttc ccagccgacc cggctggacc
      901 tgatacgcct gctcattgcc cagggggcga acggcctgcc cgccggcgag atcgccagcc
      961 gcctcgggtt gccggcatcc accctgtcct tccatctcgc cgcgctggag cgtgccggcc
     1021 tgacccaatc gacccgccag ggccgccaga tcatccatgc cgcgcggatc gccgggctgc
     1081 ggcaactgct gggcttcctc accgagacct gctgcggcgg ccggccggaa ttgtgcggcg
     1141 acatccaccg gctgctgccg cccttgcccg acgaggcccc aggcctgatg cccgccttca
     1201 acgtgctgtt cctgtgcacc cacaattccg cccgctccat catggccgag gccatcctgg
     1261 agcaggtcgg cgccggccgg ttccgcgcct actcggccgg ctccgacccg atccccgcgc
     1321 cgctgccgga ggttctcgag aagctccgcg ccttcggcca cgatgtgtcc ggcctgcaca
     1381 gcaaatcctg ggacgcgttc cttggaccgt cagcaccggc gatggacttc gtcatcagcc
     1441 tctgcgacac gccagagggc caggtctgcc cagatttcag cgaagtcgcc gttaccgccg
     1501 cctggcccct gcccgatccg gccaagttca ccggcagtgc cgtcgaacgc gcgtccatgc
     1561 tcaatgagct gtatgccagc ctgcgccgcc ggatggagat cttcaccgcg ttgcccttcg
     1621 cctcgctcga ccgcatggcc attcgtgccc ggctcgacga gatcggcggc gggctgcggc
     1681 cacctctggg agcacgttga gcatgcgcgt cggcatcaat ggcatgggcc gcatcggccg
     1741 cctggctttg cgcgccgcgc tgggtaggat ttaccgcccg gcagatgatc cccgcgcgga
     1801 taaccggttg gacgtggtgc acgtgaacga actgcgcggc ggagcggccg ccacggccca
     1861 cctgctggag ttcgacagcg tgcacggccg ctggcgcgac agcttcgggg tggagggcga
     1921 tcatgccatc catatcggca accgccgcat cggcttcagt gccgaacccg aggccggtgg
     1981 cgtggcgtgg ggcgagctgg gctgcgacct cgtgctggaa tgcaccggca agttcctgaa
     2041 gcaggaacag ctcgccggct acttcgcccg cggggtcaag cgggtgatcg tcgccgcacc
     2101 ggtgaaggac ggcagcgcac tcaacatcgt cgtcggcgtc aacgacgatc gctacgaccc
     2161 gcgcgagcac cggctgctga ccgcggcgtc ctgcaccacc aactgcctcg ccccggtggt
     2221 caaggtgctg catgaggcgg tcggcatccg tcatggccag atcaccacca tccacgatcc
     2281 gaccaacacc aacgtggtga tcgacgcacc ccaccatgac ctgcggcgtg cgcgttcggc
     2341 catgctgtcg ctgtcgccca ccaccaccgg cagcgccacc gccatcgcgc tgatctaccc
     2401 cgaactgaag ggccggttga acggccacgc ggtacgcgcg ccggtactca atgccagcct
     2461 gacggactgc gtgttcgaat tgatgcggcc gactgatgtt gacgcggtga acgggctctt
     2521 tcgcgcggcg gctgccgggc cgctcgcggg tatcctgggc ttcgagaccc gcccgctggt
     2581 ctccgccgat tacgccaacg acccgcgcag cgcgatcatc gacgctccca gcacgatggt
     2641 caccgacggc acgctggtga aggtctatgc ctggtacgac aacgaggtcg gctacgcctg
     2701 ccgcatggtg gacctcgcca acatcgtcat gcagcggggg atctgatgtc gtccggactg
     2761 cgcaactacg ccatcgtcac caccgcctac ttcctgttca ccatcaccga cggcgcactg
     2821 cgcatgctgg tgctgctgca cttctacagc ctgggctaca cgccgttcac cctggcgctg
     2881 atgttcctgc tgtatgagac ggcaggcatc ttcgccaatg cgggcggtgg ctggatggcc
     2941 tcgcgctacg gcattccgcg catgctgatg gccggccaga tgttgcagat tggcgggttg
     3001 tgcatgctgt cggcgctgca gccgggttgg agcgccgcgc tgtcggtcgc ctgggtggtg
     3061 ctggcgcagg gcgttgcggg cgttgccaag gacttcacca agacctcctc gaagagcgcg
     3121 atcaaggcgt ctgccacgca ggacatgggg cagctgttcc gctgggtggc ctggttcacc
     3181 ggatcgaaga atgccaccaa gggcgccggc ttcttcatcg gcggcttcct gttgcaggtc
     3241 gccggcttcc aaccggcgct gtggctgatg gcggcgatgc tcgctctggt gctggccggc
     3301 gtcgcattgt cgctgccgcg cacgctgggc aaggcgaaat cctcgaagag tttccgcgag
     3361 ttgttcgcca agagccgagc ggtcaacctg atcgccgcgg cccgcatctt catgttcggc
     3421 tcgcgggatg tgtggttcgt ggttgcactg ccggtgtatc tctatggatt cggctggcag
     3481 tacatgcagg tggccggctt cgtcgcggcc tggaccatcg cctatggcgc ggtgcaggcg
     3541 ctggcgccgc aggtggtcac ccgcagccgc gacgggttga gccgggaggt accggccgcg
     3601 cgcctgtggg gcgccatcct gaccgtcatc cccgtggcac tggcgctgct gttgaccatg
     3661 cccggggtgc cgcgcccgga cctgatcctg gtcgtcgggc tgctggtgtt tggctttgtt
     3721 ttcgcggtga tctcctcgct gcattcctac ctgatcctgg cctatgcggg atcggagaag
     3781 gcggccgagg atgtcggctt ctattacgcc gccaacgcgg ccgggcggct gatggggatc
     3841 atgctgtcgg gcctgctgac cgagaccggc
//
LOCUS       DASSZH010000467         2151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_965798, whole genome shotgun sequence.
ACCESSION   DASSZH010000467 DASSZH010000000
VERSION     DASSZH010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965798"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VFN42_10090"
     CDS             complement(<1..619)
                     /locus_tag="VFN42_10090"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET8997004.1"
                     /translation="MMRIDSAAPEPSRPPRIALALQGGGAHGAFTWGVLDRLLEDGLR
                     PDRICGVSSGAIIGTVMAQGLVRGGPEGGRAEMRRLWRHVAQAHRLSLLQNGPLERWL
                     WGWDLSNSLAWRGLEMAMRLFAPAQFNPFGHNPLRPLLSGLLEPALLTHPAAPKLTVA
                     ATDVATGQAVLFDNAAISVDVLLASACLPFVFPAVEIDGRAYWDGG"
     gene            718..996
                     /locus_tag="VFN42_10095"
     CDS             718..996
                     /locus_tag="VFN42_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET8997005.1"
                     /translation="MIRSFGDKRTQALFEDAFVSDFQGVARRAKRKLEAVNAAARLAD
                     LGVPPSNRLERLQGRWKDFHSIRINDQWRIVFRWLDGNAHDVRVVDYH"
     gene            1009..1311
                     /locus_tag="VFN42_10100"
     CDS             1009..1311
                     /locus_tag="VFN42_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HET8997006.1"
                     /translation="MAEQEFAPVTPGEMLKEEFLAEFNLSQAELARAIGISPNRITEI
                     VNNRRRITADTALRLSLYFGNSPEFWMNLQTSYDLKLARRGLTSKEASRIQAQRAA"
     gene            complement(1318..>2151)
                     /gene="amrS"
                     /locus_tag="VFN42_10105"
     CDS             complement(1318..>2151)
                     /gene="amrS"
                     /locus_tag="VFN42_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HET8997007.1"
                     /translation="GTAGCNLTCKFCQNWDISKAREADRLNQHAGPGQIAAIARQLGC
                     RSVAFTYNDPVIFLEYAADTARACHDAGLKTVAVTAGYITQQARGDFYQHIDAANVDL
                     KAFTEPFYHRLCGGHLKDVLETLVWLRHETKVWVEITTLLIPGENDSDAELAALSSWI
                     AAELGPDVPLHFSAFHPDWRMQDHPPTPPATLTRARAIAMGRGLRYVYTGNVHDASGG
                     STYCHACGNLLIERDWYQLGRWGLDAAGRCKQCGTAAAGVFEEQPGDWGRKRQPVRFA
                     A"
BASE COUNT          371 a          709 c          713 g          358 t
ORIGIN      
        1 agccgccatc ccaataggcg cggccgtcga tctccaccgc cgggaagacg aacggcaagc
       61 aggccgaggc cagcagcacg tccacgctga tcgccgcatt gtcgaacagc actgcctggc
      121 cggtcgccac gtcggtcgcc gcaaccgtca gcttcggtgc ggcgggatga gtcagcagcg
      181 cgggttccag caagccggac agcagcgggc gcagtggatt gtggccgaac gggttgaact
      241 gcgccggcgc gaacagccgc atcgccatct ccagcccgcg ccaggccagg gagttggaca
      301 ggtcccagcc ccacagccag cgctccagcg ggccattctg cagcaggctc agccggtgcg
      361 cctgcgccac gtgccgccac agccggcgca tttcggcgcg cccgccctcc gggccgccgc
      421 gcaccaggcc ctgcgccatc accgtgccga tgatcgcgcc ggaggacacg ccgcagattc
      481 ggtcggggcg gagcccatcc tccagcaacc ggtccagcac gccccaggtg aaggcgccgt
      541 gcgcgccacc gccttgcagg gcgagggcga tgcgcggtgg acggctgggt tcaggcgcgg
      601 cggaatcgat cctcatcatg gggccaaaat ggcgttgtcg tctggagacc gcaaatgagc
      661 aggtagatcc ctcctgggtt cgccttgatc gcataccggt acgcggtata acggcgcatg
      721 atccgttcgt tcggagacaa gcgcacgcag gccctgtttg aagatgcgtt cgtgtcggat
      781 ttccagggcg tggctcggcg ggccaagcgg aaattggaag ccgtgaatgc tgctgccaga
      841 ttggctgatc tcggcgtgcc gccatccaac cgcttggagc ggctgcaggg tcgctggaag
      901 gatttccatt cgatccggat caatgaccaa tggcggattg tgtttcgctg gctcgacggt
      961 aacgcgcacg atgtgcgcgt tgttgactat cattgaagga ggactgcgat ggctgagcaa
     1021 gaatttgccc cggtcacgcc gggggagatg ctgaaagagg agttcctcgc agagttcaac
     1081 ctctcgcagg cggagctcgc ccgagcgatc gggatctcgc cgaaccgcat caccgagatc
     1141 gtcaacaatc gccggcgtat caccgcggat accgccctgc gcctgagcct gtatttcgga
     1201 aacagcccgg aattctggat gaacctgcaa acaagctatg atctcaaatt ggcccgccgc
     1261 ggcctgacca gcaaggaggc gtcccgcatc caggcgcagc gcgcagcata gacgtcctca
     1321 cgccgcgaag cgcaccggct gccgcttgcg gccccagtcg cctggctgtt cctcaaacac
     1381 gcccgccgcg gcggtgccgc attgcttgca gcgccccgcc gcatccaatc cccagcggcc
     1441 gagctggtac cagtcgcgct cgatcagcag attgccgcag gcatgacaat aggtgctgcc
     1501 accggacgca tcgtgcacgt tgcccgtata gacgtagcgc aggccacgcc ccatcgcgat
     1561 cgcccgcgcc cgggtcagcg ttgccggcgg tgtcggcgga tggtcctgca tgcgccagtc
     1621 gggatggaag gcgctgaaat gcagcggcac gtcggggccg agttccgccg cgatccagct
     1681 cgacagcgcg gcgagttccg cgtcgctgtc gttctcgccg gggatcagca gcgtggtgat
     1741 ctcgacccac accttggttt cgtgccgcaa ccagaccagc gtttccagca cgtccttcag
     1801 atggccgcca cacaggcgat ggtagaacgg ttcggtgaac gccttcaggt ccacgttcgc
     1861 cgcgtcgatg tgctggtaga aatcgccgcg tgcctgctgt gtgatgtagc cggcggtgac
     1921 cgccaccgtc ttcaatccgg cgtcgtggca ggcgcgcgcg gtatcggcgg cgtattccag
     1981 gaaaatgacc ggatcgttgt aggtgaacgc caccgaccgg cagccgagct gccgggcgat
     2041 cgcggcgatc tgcccgggtc cggcgtgctg gttcagccgg tcggcttcgc gggccttgga
     2101 gatgtcccag ttctggcaga atttgcaggt caggttgcag ccggcggtgc c
//
LOCUS       DASSZH010000468         8960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1289447, whole genome shotgun sequence.
ACCESSION   DASSZH010000468 DASSZH010000000
VERSION     DASSZH010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8960
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289447"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1345
                     /locus_tag="VFN42_10110"
     CDS             <1..1345
                     /locus_tag="VFN42_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET8997008.1"
                     /translation="AKRIPELVAHAVDVATSGRPGPVVIALSEEMQKELAEVPDLPRV
                     PVAPAHPDPAALLQLRAMLAKARRPVAVLGGSCWNEAGRASIRDFLIGNDIPVTTGFR
                     RQALYDGTQDNFAGDLGVGSDPHLIGKVKEADLILAIGTRLGDAVTQGYTLLDMAGSV
                     PIIQVHPDGAEIGRVFRPALGIVSDLNTFAEAMAAMEPVQPHWTRWTEELRSLREAQR
                     AVPNYEGTLNLGTCMRELEGMLAPDAIVTTDAGNFAGWATRFLNFREDQRYIGPTNGA
                     MGYSVPAAIGAKVTFPDRMVVSMVGDGGFMMTGQELATAFHHGVAPIVLLFNNQMYGT
                     IRMHQERAYPYRVSGTALTNPDFAKYIEAFGGHGEVVTETAEFSAAFQRAVESGKPAV
                     IELRVNPEQITTRQTIAQLRAGKTPPKGAAKPKRAAAPKRAAPTRSAASNTKKRG"
     gene            1403..2548
                     /locus_tag="VFN42_10115"
     CDS             1403..2548
                     /locus_tag="VFN42_10115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET8997009.1"
                     /translation="MRNADVLLANVGKVADAWQSQRSERQRRRQLDPDDFRQLQALGL
                     PLAAVPAEFGGLWESTQRSVRPLCEALRCLARGDPSVALSASMHPGVLAFWRESPPLQ
                     PASTAWEAQKQWVYQTALDGAWWGTITSESGSGGDVGKTRAMARPDAAGYRITGEKHF
                     GSGSGATSYMLTTAVPEGEDSPAWFFLDLRDTPWDGSTGVSLIAAWDGHGMAATNSHS
                     FAFRDFPATRLAWPGSWREVLGGGGTVPMFYTAVIVGVVDAAMDYARQQMQSAGWLAT
                     RAFEKVEWVGAEREAWLLRQAYEGGLQALERLGRARDDTALAKANVALLAESVLTRLC
                     RISGGSAFSRRGPLGFWFEDVRALGFLRPPWGLGLEALFDMSAARPA"
     gene            2545..4161
                     /locus_tag="VFN42_10120"
     CDS             2545..4161
                     /locus_tag="VFN42_10120"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET8997010.1"
                     /translation="MTTSAESPSAPAIEDAARCYRERDYAGAEAIAQAIVAAEPRHFD
                     ALHLLGVLCINAKRPADAAGYLRRALAERPTNAEAQYHLGNAMLDLQLYDAAQVAFET
                     ALRLAPGHYDATNNLGNALAGAGRHEEAIERFREALLIRPHAAPTLYNLGQSLVALGR
                     YDEAVAQYRAALDYAGDAPPDRLAGVVAGLCRAFVDQERYAEALAACRDVPPPLFGHP
                     VIEWNESLTRLVLGDYRAGWRLYESRFLVPEHDPPRPGAQVLDLRTVAGRRVLVFGEQ
                     GRGDLIQFARYLPMLAARGAEVIAEIYPDLHRLFAGIDGVVQVVEPESPLPPCDLATP
                     LLSLPLAFGTELDSVPATVPYLAPPPDLAQAWAARLGSAERPRIGLTWRGSSFSASRT
                     SFPASALAPLVEIGGLAFHAVQTPLTASDEDWLARHPQIQVHAADLGDFADTAALLMH
                     MDLVISVDTSVAHLAGALGRPTWVLLPVSPDWRWMARRADSPWYPTVRLFRQQRRGDW
                     TELLRRVAVALAGLDFPAADSAGAQSRGWT"
     gene            complement(4158..4370)
                     /locus_tag="VFN42_10125"
     CDS             complement(4158..4370)
                     /locus_tag="VFN42_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HET8997011.1"
                     /translation="MADHVYKVVELVGSSGSSHAEAIENAIARAGKSVRNLRWFEVIQ
                     MRGEIEQGRIQHYQVTLKAGFTLDEG"
     gene            complement(4455..4919)
                     /gene="tssJ"
                     /locus_tag="VFN42_10130"
     CDS             complement(4455..4919)
                     /gene="tssJ"
                     /locus_tag="VFN42_10130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HET8997012.1"
                     /translation="MTRRRSLLLLPAALLLARCGGARKPPGMLTLEVSGSPDQNPDSA
                     GDPEPVAVRIYQLRAIRKFETSGWAALTEREQPTLGADDAGSQEVVVAPEQNVRRTIL
                     LQPDVQAIGIVVLYRDIGHAQWRALAPVAPTGNTRLAVHVGKQAVSLVAPPG"
     gene            complement(5039..5788)
                     /locus_tag="VFN42_10135"
     CDS             complement(5039..5788)
                     /locus_tag="VFN42_10135"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8997013.1"
                     /translation="MSVEGKVVLITGAARGLGMEYARSLGAAGARIVAGDVLDCDAAA
                     EAAGNGALAVRLDVTDIASADAMAELAMEAFGRIDALINNAALYGSLRGGRFNTIAEA
                     DWDAAMAVNVKGIWNCCKAVVPAMRQSGGGSIINVASLAATYGMPFALHYTTSKAAVI
                     GLTRGLARELGRDNIRVNALAPSAVMTEGTAEFFGDKLERGLEVIRSSQSIQRTLEPA
                     DLTGTVTWLVSDASRFVTGQTIAVDGGTVML"
     gene            complement(5864..6616)
                     /gene="radC"
                     /locus_tag="VFN42_10140"
     CDS             complement(5864..6616)
                     /gene="radC"
                     /locus_tag="VFN42_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HET8997014.1"
                     /translation="MKRVRRTIPELGESLDLLAPLEAEPASGSAGTDGHRARLRARLL
                     TAGPDALADHEMLEIVLFALPRRDTKPLARMLLERFGSFAAAIAAPVAELLAVDGLGE
                     TGAAALKTVQAAALRLERAGVMQRPVLACWDRLIAYLTAEMALDKVEQVRVLFLDNRN
                     RLLADEAHGRGTVNHTPVYPREVVKRALELHATALILVHNHPSGDPTPSREDIAMTRE
                     IQAAAELMRITLHDHVVIAAGSWLSFRQEGLL"
     gene            complement(6653..7057)
                     /locus_tag="VFN42_10145"
     CDS             complement(6653..7057)
                     /locus_tag="VFN42_10145"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8997015.1"
                     /translation="MSATTLSPTPAVAKKSQAQHVAGRRAFFKYRDLGVTAATGGKLR
                     AQVTSAEAGMSKPTGWHVHKCEAQLVYMLSGWIELEFAGEPLCRLEAGDSILIPGNTP
                     HNEIRTSDAFELLEVSLPAEMGTEPCDPPAGH"
     gene            7198..7548
                     /locus_tag="VFN42_10150"
     CDS             7198..7548
                     /locus_tag="VFN42_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003624223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET8997016.1"
                     /translation="MSKIIRTEPGSILSKAVEYHGFVYLPGVTARDTGKDIKGQTADV
                     LAQIDDLLEQHGTDKTRLLQAQIWVKHISDRGAMNEVWTPWLPSGGAPVRACVQAELA
                     SPELLVEIMVTACK"
     gene            complement(7785..8126)
                     /locus_tag="VFN42_10155"
     CDS             complement(7785..8126)
                     /locus_tag="VFN42_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997017.1"
                     /translation="MTDPAGAGEVDCMPKVTNNGTMLAWCNVAVFDVERHLPDMGLPV
                     DAGVPDKVSHDVGLSENVTYRMMRGGLGGRRGKARYEGEKSTQNQGRDRETCITHRMY
                     SPRFCPSRERR"
     gene            8019..8711
                     /locus_tag="VFN42_10160"
     CDS             8019..8711
                     /locus_tag="VFN42_10160"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HET8997018.1"
                     /translation="MALHVEHRHIAPSKHRAIVRHLGHAIHLASTRRVSHFVGISCVP
                     FARQDSGISLPGNAWEWWGNAAGVYSRGRVPQPGAVLAFRSNPHMPLGHVAVVTKVIN
                     PREIEIDQANWVHGRVTRGVPVVDVSEANDWTAVRVEMGHSGSFGSVYPTYGFIYDKP
                     DNGTVLAAAHAPAPQPVLNAAPSDLRPVAEQPWQTYEEVAELPSGPRHYVLPHKVLTM
                     RHAHARTRVADR"
BASE COUNT         1378 a         3089 c         3037 g         1456 t
ORIGIN      
        1 ggcgaagcgc atcccggagc tggtggcgca tgcggtcgat gtcgcaacct ccggtcgtcc
       61 cggaccggtg gtgatcgcgc tttccgagga gatgcagaaa gagctggcgg aagttcccga
      121 cctgccgcgc gtgccggtcg cacccgcgca tcccgatccg gccgcgctgc tgcaactccg
      181 cgccatgttg gcgaaggccc gccgcccggt cgcggtgctc ggcggttcct gctggaacga
      241 ggccgggcgc gcgtcgatcc gcgacttcct gatcggcaac gacatcccgg tgaccaccgg
      301 ctttcgccgc caggcgctgt acgacggcac gcaggacaat ttcgccggcg acctgggcgt
      361 gggctccgat ccccatctga tcggcaaggt gaaggaagcc gacctgatcc tggcgatcgg
      421 cacaaggctg ggcgacgcgg tgacgcaagg ctacacgctg ctcgacatgg ccggcagcgt
      481 gccgatcatc caggtgcatc cggatggtgc ggaaatcggc cgcgtgttcc ggccggcgct
      541 gggcatcgtc tccgacctca atacgttcgc cgaggcgatg gcggcgatgg aaccggtgca
      601 gccgcactgg acccggtgga ccgaggaact gcgcagcctg cgggaggccc agcgcgcggt
      661 gccgaattac gaaggcacgc tgaacctcgg cacctgcatg cgcgagctgg aaggcatgct
      721 ggcgcccgac gccatcgtca ccaccgatgc gggcaacttc gccggctggg ccacgcgctt
      781 cctgaacttc cgcgaggatc agcgctacat cggcccgacc aacggcgcga tgggctatag
      841 cgtgccggcg gcgatcggcg ccaaggtgac gttccccgac cgcatggtgg tcagcatggt
      901 gggcgacggc gggttcatga tgaccggcca ggaactcgcc acggcgttcc accacggcgt
      961 ggcgccgatc gtgctgctgt tcaacaacca gatgtacggc acgatccgca tgcaccagga
     1021 gcgcgcctat ccgtatcgcg tctccggcac cgcgctgacc aatccggatt tcgcgaaata
     1081 catcgaggca ttcggcggac acggcgaagt ggtgaccgag accgcggaat tctccgcggc
     1141 attccagcgc gcggtggaaa gcggcaagcc ggcggtgatc gaattgcgcg tgaacccgga
     1201 gcagatcacc acgcggcaga ccatcgccca gttgcgtgcc ggcaaaactc cgccgaaggg
     1261 cgcggccaag ccaaagcggg ccgcggcgcc caagcgtgcc gccccgaccc gcagcgctgc
     1321 gtccaacacc aagaagcgag gctgatgcgg cgttgacgcc tgggtgaccc tctgctcgat
     1381 ccgaaggtga cgggaggcag taatgcgcaa tgccgacgtg ttgctggcga atgtcgggaa
     1441 ggttgcggac gcctggcagt cgcagcgatc cgaacgccaa cgccgtcggc agctcgaccc
     1501 cgacgacttc cgccaattgc aagccctcgg gttgccgctg gctgccgttc cagcggaatt
     1561 cggcggattg tgggaatcca cccagcgctc cgtgcggcca ttgtgcgaag cgttgcgttg
     1621 tctcgcgcgg ggcgatccgt cggtggcctt gtccgcctcc atgcatcccg gagtgctggc
     1681 gttctggcgc gagtcgcccc cgctgcaacc cgcctcgacg gcatgggagg cacagaagca
     1741 gtgggtatac cagacagcgc ttgacggcgc ctggtgggga accatcacct cggagtccgg
     1801 cagcggcggc gatgtcggca agacccgcgc gatggcgcgc cccgacgcgg ccggctaccg
     1861 gatcaccgga gagaagcact ttggctcggg ttccggggcg acctcctaca tgctgaccac
     1921 cgcggttccg gaaggcgagg actcgccggc ctggttcttt ctggatctcc gcgacactcc
     1981 ctgggacgga tcgacgggtg tgtccctgat cgcggcatgg gatggccacg gcatggcggc
     2041 aaccaacagc cactccttcg ccttccggga tttccccgcg acccggttgg cgtggccagg
     2101 tagctggcgg gaggtgctgg gcggtggtgg cacagttccc atgttctaca cggcggtcat
     2161 cgtcggcgtg gtggatgccg cgatggacta cgcgcggcag caaatgcaaa gcgccggctg
     2221 gttggccacg cgtgccttcg agaaggtgga atgggtcggc gcggaacgcg aagcatggtt
     2281 gctgcgccaa gcttatgaag gcggcttgca agcgctggaa cgtcttggca gggcgcgcga
     2341 tgacaccgcg ctggcgaagg ccaacgtcgc cctgctcgcg gaatccgtct tgacgcggct
     2401 atgccgtatc tccggcggca gcgccttttc ccgccgcgga ccgctcggct tctggttcga
     2461 ggatgtgcgt gctctgggct tcctgcggcc gccctggggc ctggggttgg aggcgctgtt
     2521 cgatatgagc gccgctcggc ctgcgtgacg acgagcgccg aatccccctc cgcgcccgca
     2581 atcgaggacg ctgcgcgctg ctacagggag cgcgattacg ccggcgcgga agccatcgcc
     2641 caagcgatcg tcgccgccga gccgcggcac ttcgacgcgc tccacctgct gggcgtgctg
     2701 tgcatcaatg ccaagcggcc agccgacgcg gcgggatatc tgcgccgcgc cctcgccgag
     2761 cgtcccacca atgccgaggc gcagtatcac ctcggcaatg cgatgctgga tctgcagctt
     2821 tatgatgccg cacaggtcgc attcgaaacc gcgctccggc tggctccggg gcactatgac
     2881 gcgaccaaca acctgggcaa cgcgctggcc ggggccggcc ggcacgagga ggcgatcgag
     2941 cgctttcgcg aggcgctgct gatccggccg catgccgcgc ccacgctgta caacctgggg
     3001 cagtcgctcg ttgcactcgg acggtatgac gaggcggtgg cgcaatatcg cgccgcactg
     3061 gactacgctg gcgatgcacc gcccgatcgg ctggcgggcg tggtcgccgg gttgtgccgc
     3121 gccttcgtcg atcaggaacg ctacgcggag gcgctggcgg cctgccgcga tgtgccgccc
     3181 ccgctgttcg gccatccggt gatcgaatgg aacgagagcc tcaccaggct ggtgttgggc
     3241 gactatcgtg ccggctggcg cttgtatgaa agccgcttcc tggtgccgga gcatgatccg
     3301 ccgcgccccg gcgcgcaggt actcgacctg cggacggtcg ccgggcggcg ggtgctggtg
     3361 ttcggtgagc aggggagggg cgacctgatc cagttcgccc gctacctgcc gatgctcgcc
     3421 gcgcgcggtg ccgaagtgat cgccgaaatc tatcccgacc tgcaccggct gttcgccggc
     3481 atcgacggcg tggtgcaggt ggtcgaaccc gagtccccgt tgccgccctg tgacctcgcg
     3541 acgccgctgc tcagcctgcc gctggctttc gggacagagc tggacagcgt gccggccacg
     3601 gtgccctacc tggcgccgcc gcccgacctg gcacaggcct gggcagcacg gctcggctcc
     3661 gccgaacggc cgcggatcgg cctgacgtgg cgcggttcga gcttcagcgc atcgcggacc
     3721 tcgtttcccg catccgcgct ggcgccgctg gtggagattg gcggcctcgc attccatgcc
     3781 gtgcagacgc cgctgaccgc gtccgacgag gattggcttg cgcgccatcc gcagatccag
     3841 gtgcatgcgg cggacttggg cgacttcgcc gacacggcgg cgctgctgat gcacatggac
     3901 ctggtcatca gcgtcgacac ctcggtggcc cacttggccg gcgcactcgg ccgcccgacc
     3961 tgggtcctgc tgcccgtcag cccggactgg cgctggatgg cgagacgcgc ggacagcccc
     4021 tggtatccga cggtccgcct gttccgccaa cagcgccggg gcgactggac cgagctgctg
     4081 cggcgcgtcg cggtggcgct tgccggactg gacttccctg cagcggattc cgccggcgcg
     4141 cagtcccgcg gctggaccta accctcgtcc agggtaaagc ccgccttcag cgtgacctga
     4201 tagtgctgga tgcggccctg ttcgatttcg ccccgcatct ggatgacttc gaaccagcgc
     4261 aggttgcgca cggacttgcc ggcccgggca atggcgttct cgatcgcttc ggcgtggctg
     4321 gagccggacg acccaaccag ctcgacgacc ttatagacat gatcggccat ggcagagatc
     4381 cctcacgcga tggatgacgc ggagggaacg cagcagaacc ggtccggtcg catcgcaacc
     4441 gggccgatcc ggcgtcagcc gggcggcgcc accagcgaca cggcctgctt gccgacatgc
     4501 accgccagcc gggtgttgcc ggtgggcgcc accggcgcca gggcgcgcca ttgcgcgtgg
     4561 ccaatgtcgc gatacagcac gacaatgccg atcgcctgca catccggttg caggaggatg
     4621 gtccggcgca cgttctgttc cggtgcgaca accacctcct gcgagccggc atcgtcggcg
     4681 cccagcgtcg gctgttcgcg ttcggtcagt gcggcccagc cgctggtttc gaatttgcgg
     4741 atcgcgcgta gctggtagat gcgtaccgcc accggctctg gatcgccggc gctgtcgggg
     4801 ttctgatccg ggctgcccga cacttccagc gtcaacatcc cgggcggctt gcgggcgcct
     4861 ccgcatcgcg ccagcagcag cgcggcgggc aacagcagca gcgatcggcg gcgggtcatc
     4921 gtctctcctt caggcgtatc ccattgattg cccggggtaa cagcggccac cgcggccgac
     4981 gccgtcccgg gccgtatcgc ccggcccgga accgcccagg ccatacgtgc ggcgcgcctt
     5041 acagcatcac cgtgccgcca tccaccgcga tggtctgccc ggtcacgaag cggctggcgt
     5101 ccgacaccag ccaagtcacc gtgccggtca ggtccgccgg ttccagcgtg cgctggatcg
     5161 actggctgga acggatcacc tccagcccgc gctccagctt gtcgccgaag aattccgcgg
     5221 ttccctcggt catcaccgcg cttggtgcca gcgcgttcac ccggatgttg tcgcggccca
     5281 gctcgcgcgc cagcccgcgc gtcagcccga tcaccgcggc cttggacgtg gtgtaatgca
     5341 gcgcgaaggg catgccgtag gtggcggcga gcgacgccac gttgatgatg gaaccgccgc
     5401 cggactggcg catcgccggc accaccgcct tgcagcaatt ccagatgccc ttgacgttca
     5461 ccgccatcgc ggcgtcccag tcggcttcgg caatggtgtt gaaccggccg ccgcgcagcg
     5521 aaccatacag cgcggcattg ttgatcagtg cgtcgatccg gccgaaggcc tccatcgcca
     5581 gttcggccat tgcgtcggcc gaggcgatgt cggtgacgtc cagccgcacc gcgagtgcgc
     5641 cgttgcccgc tgcctccgcc gcggcatcgc agtccagcac gtcgccggcc acgatgcgcg
     5701 ccccggccgc gcccaggctc cgcgcatatt ccattcccag cccgcgggcg gcgccggtga
     5761 tcaacaccac tttgccttca accgacatag ccgcgtcctc ctgtgccagg gtcgggcgcg
     5821 gacgatgcgg cgcggcgcgc gggggcgcaa tcccatgccg gcgctacagc agcccctcct
     5881 gccggaagct cagccagctt ccggcggcga tcaccacgtg gtcgtgcagc gtgatgcgca
     5941 tcagctcagc cgccgcctgg atctcccgcg tcatcgcgat gtcctcgcgt gacggcgtgg
     6001 gatcgccgct ggggtggttg tgcaccagga tcagcgcggt ggcgtgcagt tccagcgcgc
     6061 gcttgaccac ttcgcgcgga tagaccggcg tgtggttcac cgtgccgcgg ccatgcgcct
     6121 catcggccag cagccggttg cggttgtcca ggaacagcac gcgcacctgc tcgaccttgt
     6181 ccagcgccat ttcggcggtt aggtaggcga tcagccgatc ccagcaggcg agcaccggcc
     6241 gctgcatcac cccggcgcgt tccagccgca gtgccgcggc ctgcaccgtc ttcagcgccg
     6301 cggcgccggt ctcgcccagg ccgtccaccg ccaacagttc cgccaccggc gcggcgatcg
     6361 ccgcggcgaa gctgccgaac cgctccagca gcatgcgagc caacggcttg gtgtcgcgcc
     6421 gcggcagggc gaatagcacg atctccagca tttcgtggtc ggccagcgcg tccggcccgg
     6481 cggtcagcag gcgagcacgc agccgggcgc ggtggccgtc agtgccggcg ctgccggacg
     6541 ccggttcggc ttccagcggc gccagcaggt cgaggctttc gcccagctcc ggaattgtcc
     6601 gccgcacccg cttcatgggc cggcagcatg ccaaagccgg gttaacggaa ggtcagtggc
     6661 cggcgggtgg atcgcagggt tcggtgccca tctcggccgg cagcgagacc tccagcagtt
     6721 cgaatgcgtc ggaggtgcgg atctcgttgt gcggcgtgtt gccggggatc aggatcgaat
     6781 cgcccgcttc cagccggcac agcggttcgc cggcgaattc cagctcgatc cagccgctga
     6841 gcatgtagac aagctgcgcc tcgcatttgt ggacatgcca gccggtgggc tttgacatgc
     6901 cggcttccgc cgaggtgacc tgggcgcgca gcttgccgcc ggtggcggcg gtgacgccaa
     6961 ggtcgcggta cttgaagaag gcgcggcggc cggcgacgtg ctgggcctgg ctcttcttgg
     7021 cgaccgcagg cgtcggcgac agggtggtgg cgctcatcgg acgtcctcct ttcggctgtg
     7081 gcttgccggt gagcctagga cccggcgggc cgcttggcga gggtgcttgc tggcctgcgg
     7141 gcggcgcttg gcctagaagc cgcggagccc agcaacagcc agagaggcgt cccaggcatg
     7201 agcaagatca ttcgtaccga accaggcagc atcctgtcca aagctgtcga gtaccacggc
     7261 ttcgtctacc tgccgggcgt caccgcccgc gacaccggca aggatatcaa aggccagacc
     7321 gcggacgtgc tggcgcagat cgacgacctg ctggaacagc acggcaccga caagacgcgg
     7381 ctgctgcagg cgcagatctg ggtgaagcac atcagcgacc gcggcgcgat gaacgaggtg
     7441 tggaccccct ggctgccgtc cggcggcgcc ccggtgcgtg cctgcgtgca ggccgagctg
     7501 gccagcccgg agctgctggt tgagattatg gtgacggcct gcaagtgaag gtagcggcta
     7561 ccccgccgca actcgcggcg gggttggcct gtcggaatgg ttctgacgga accgtgaagc
     7621 ggtgcgggag tccgttcccg gcccctctcg catcagcatc aaactttggc gatttaggag
     7681 tttgttcccc gcattgccgt ttatccacag gtgatttcga acgaactgcg gcgcccaagc
     7741 cacaatcggt tcgtcactgg gttgccatag acgcaaacct gctgctaccg ccgctcacgg
     7801 ctggggcaaa aacgcgggga atacattcga tgcgtgatgc aggtttcgcg atcacgaccc
     7861 tgattctggg tactcttttc accttcgtat ctggctttac cgcggcgccc gcccaggccg
     7921 ccacgcatca tccgatacgt cacgttttcc gaaagaccca cgtcgtggct caccttgtcc
     7981 ggcacgcccg cgtcaaccgg gaggcccatg tccggaaggt ggcgctccac gtcgaacacc
     8041 gccacattgc accaagcaag catcgtgcca ttgttcgtca ccttgggcat gcaatccacc
     8101 tcgccagcac ccgccgggtc agtcatttcg tcggtatctc ctgtgtcccg ttcgcccgcc
     8161 aggattccgg catctcgctt ccgggcaatg cctgggaatg gtggggcaac gccgcggggg
     8221 tctacagccg cggccgcgtt ccacaaccag gggctgtgtt ggccttccgt tccaacccgc
     8281 atatgccgct cggccatgtc gcggtggtca ccaaggtgat caatccgcgg gaaatcgaaa
     8341 tcgaccaggc taactgggtg cacggccgtg tcacccgcgg cgtcccggtg gtggacgtgt
     8401 ccgaagccaa cgactggacc gcggtgcggg tggagatggg ccactccggc tcgttcggca
     8461 gcgtctaccc cacttacggt ttcatctacg acaagccgga caacggcacg gtcctggcag
     8521 cggcgcacgc gccggcgcca cagcccgttc tgaacgccgc gcccagcgac ctgcgcccgg
     8581 tcgcggagca gccctggcag acctatgagg aagtggccga actgccatcc ggcccgcggc
     8641 actatgtgct tccgcacaag gtgctgacga tgcggcacgc gcatgcgcga acccgggtgg
     8701 cggaccgcta gtacatcccg ccgaggcaca ttcggcggcg tcatcgccgg gcttggcgtc
     8761 agggccgaag cccattccgt catcgccggg cttgatccgg ccatccaccc ggcaacgatc
     8821 ggggcagtac gtaacggaac ccgccttgtc gggcgtagat ggccgggtca acccccggcc
     8881 agggcgtcga tggccggcgc ctaatccacc gcctccatac gcgccatcat ggccagcgtc
     8941 tgctcgggac ccagcgcggt
//
LOCUS       DASSZH010000469         5940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_232848, whole genome shotgun sequence.
ACCESSION   DASSZH010000469 DASSZH010000000
VERSION     DASSZH010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5940
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232848"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1652)
                     /locus_tag="VFN42_10165"
     CDS             complement(<1..1652)
                     /locus_tag="VFN42_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET8997019.1"
                     /translation="MSLTHTPPLPQAVGQRGTPLAELLRRIEPASLIWLAAVAALLFL
                     VVAPLVRLLVSSFQSTDTGAFTLVNYAIAWGNVRDLKAVGNTLLYAVEVTLVSAVLAV
                     PVAWSISRTNMPAKGLVRALIIGAFVTPPYLGAIGWILLAGPNAGWLNRVWMALTGSS
                     SGFLNVYSFGGLVWVTALYSFPYIFVFASAAMDLVSTEMEEAANILGAGSLRTILRIT
                     LPLVMPAIIGGGIVVFLDTVALFGTPAIIALPARIPTMTLVLWQFFEFPVRAQAAAAY
                     AIPLIVVTLALFTVQRMLLGRKGYVALTGKGGGRRLMQLGAWRWLALGYALFVVGMAV
                     LLPYGALAQAAFSKAWGLGLSWGNLTLAHFGEVLSDQASRRTIYNSFTYSAAAACFAV
                     GLSLVISYMVTRRLTRRGSILSILCMAPLVIPGIVLGVAFYATFAPPPVALYGTGAIL
                     ILGYTTRFLPIAYVNATAGMRSLNPEMEEAVRILGGGRLRALAKVVVPLLKTNLVGSW
                     LLVFIPASRELSTALFLVAAHTRVISVMMLDLSENGSFETLAA"
     gene            complement(1679..2713)
                     /locus_tag="VFN42_10170"
     CDS             complement(1679..2713)
                     /locus_tag="VFN42_10170"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8997020.1"
                     /translation="MSLTRRAAMIALGATSLGLSAAHAADDEQQLYAAAKKEGRVTWY
                     SGFLTQPIVESVGKAFTAKYPGIQVDATKTTSQVAFQRVMQDINAGAVQADVFSSTDA
                     SHMSYLISKGKLVNYTPPNDSALVEGPRKFNGGGYYHVAWLGLVAIMYNTKHVKPEEA
                     PKDWTDLTDPKWKNRIAFGSPNFSGLVGVWTVAMNEKYGWDYFQKLNKLNPLIGRSID
                     DAVAVLNSGERHVAICADGSAFRSMAAGNPLAVNYAKSFSLVDYSPAAILKGSHSPNG
                     AKLFMNFLAGPEYSQILAKAYFQPLRGDVPPPKGATPLSQLTLYQPSLEAIEKQLPAL
                     RGKWRDTFGT"
     gene            2969..4627
                     /locus_tag="VFN42_10175"
     CDS             2969..4627
                     /locus_tag="VFN42_10175"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET8997021.1"
                     /translation="MAKVNGATVMARSLAQQGVKFMFGVVGFPVGPIAEAAQKAGITY
                     LGMRNEQSASYAAQAIGYLTGRPGACLVVSGPGVVHGLAGLANAQQNCWPMILIGGAS
                     PTYQNGMGAFQEERQVQIASPFCKFAHAVEHVHRIPFYVEMAVRNAIYGRPGACYLDM
                     PDDIIQGQVEEEQVLQAATVAEPPRFQAMPEDIEKALNTLESAERPLVIVGKGMGWSR
                     AEDEVRKFIDRTQLPFLASPMGKGVMDDTHPLSVGAARSHALQEADVIFLMGARLNWI
                     MHFGAPPRFNKNVRIVQLDIAAEAISQNVPAEVALVGDGKAIIGQLNKALETRQWFYP
                     KDTPWHKAIAEKAAANAKQIQPMIDDNSAPTNYYRAFKDISAWLPDDAVIIGEGANTM
                     DIGRTQMANKNARLRLDAGTYGTMGIGMGFTIAAAMAHPERPIVSVFGDAALGFSGME
                     IETACRYRLPVKIVVLNNGGIGSGMAEFPEDRSTLPPRVMAIGSGYEKIMDAFGGKGF
                     YVDDPKDLRAALDAAMAYDGPALVNVKLHHAAGRKPQQFHWHTA"
     gene            complement(4699..5829)
                     /locus_tag="VFN42_10180"
     CDS             complement(4699..5829)
                     /locus_tag="VFN42_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003617003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HET8997022.1"
                     /translation="MTPKIARFLAQQQPATPCLVLDMDRVEANFRALQQALPLARIYY
                     AVKANPAPQVLERLVRLSSSFDAASIEEVHACLDAGARPEAISFGNTIKKASAIRAAH
                     ERGVSLFAFDSIEELQKLAQQAPGARVYCRILVENEGADWPLSRKFGTTIDNARTLML
                     RAGEMGLDPYGLSFHVGSQQTTTRAYEMAIGKVAMLFTDLTEAGVNLRMMNLGGGFPT
                     RYRDEVPEIDQFGSAIMTAMTGHFGNALPEMLIEPGRFIVGDAGLVSAEVVLVSRRDP
                     DSPVRWVYLDIGRFGGLAETEGESIKYRIVTKHDGTAMGPVAIAGPTCDGADIMYEKA
                     NYRLPLALESGDRVELLSTGAYVTTYASQKFNGFAPLAEYYL"
BASE COUNT         1070 a         1934 c         1994 g          942 t
ORIGIN      
        1 gccgccagcg tctcgaaact gccgttctcg ctcaggtcca gcatcatgac cgaaatcacc
       61 cgcgtgtgcg cggccaccag gaacagggcg gtgctgagct cgcggctggc ggggatgaac
      121 accagcagcc aggagccgac caggttggtc ttcagcagcg gcaccaccac cttcgccaac
      181 gcgcgcaacc gcccgccgcc gaggatgcgc accgcttcct ccatctccgg gttcagcgag
      241 cgcatgccgg cggtggcgtt gacataggcg atcggcagga agcgcgtggt gtagcccagg
      301 atcaggatgg cgccggtgcc gtacagcgcg accggcggcg gggcgaaggt ggcgtagaac
      361 gccacgccca gcacgatgcc ggggatcacc agcggcgcca tgcacaggat cgacaggatg
      421 ctgccgcgcc gcgtcagcct gcgcgtcacc atgtagctga tcaccagcga caatccgacg
      481 gcgaagcagg ccgcggcagc cgaataggtg aagctgttgt agatggtccg gcgcgacgcc
      541 tggtccgaca ggacctcacc aaaatgcgcc agcgtcaggt tgccccatga caggccgagc
      601 ccccaggcct tgctgaatgc cgcctgcgcc agtgcgccgt agggcagcag cacggccatg
      661 ccgaccacga acagcgcata gcccagggcc agccagcgcc aggccccaag ctgcatcagc
      721 cgccggccgc cgcctttgcc ggtgagcgcg acatagcctt tgcgccccag cagcatgcgc
      781 tgcacggtga acagcgccag cgtcaccacg atcaacggga tcgcgtatgc tgccgcggcc
      841 tgcgcgcgca ccgggaattc aaagaactgc cacagcacca gcgtcatggt gggaatgcgt
      901 gcgggcaggg cgatgatggc cggtgtgccg aacagcgcca cggtgtccag gaacaccacg
      961 atgccgccgc cgataatcgc cggcatgacc agcggcaggg tgatgcgcag gatggtgcgc
     1021 aggctgccgg cgccaaggat gttggccgct tcctccattt cggtggaaac caggtccatc
     1081 gcggcggagg cgaagacgaa gatatagggg aacgagtaca gcgcggtgac ccagaccagg
     1141 ccgccgaagc tgtacacgtt gaggaagccg ctgctgctac cggtcagcgc catccacacg
     1201 cggttcagcc agccggcatt cgggccggcc agcagaatcc agccgatcgc gccgaggtag
     1261 ggcggggtga cgaaggcgcc gatgatcagg gcgcggacca gccccttggc cggcatgttg
     1321 gtgcgagaga tgctccaggc aacgggcacg gcgagcaccg ccgataccag cgtgacctcg
     1381 acagcgtaga gcagcgtgtt gcccacggcc ttcaggtcgc gaacattgcc ccaggcgatg
     1441 gcgtaattca ccagggtaaa ggcgccggtg tcggtggact ggaaactgga caccagcagg
     1501 cgcaccagcg gcgccacgac caggaacaac aatgcggcga cggcggcgag ccagatcagg
     1561 ctcgccggct cgatccggcg cagcagctcg gcgaggggag tcccccgctg tccgacagcc
     1621 tgcggcagcg ggggggtgtg agtcaggctc atacggggga tccggactgc gccgatgctc
     1681 aggtgccgaa cgtgtcccgc cacttgccgc gcagtgccgg caattgcttc tcaatggcct
     1741 ccagcgacgg ctggtacagc gtgagctggc tcagcggcgt ggcgcccttc ggtggcggaa
     1801 cgtcgccgcg cagcggctgg aaataggctt tcgccaggat ctgcgaatat tccggacccg
     1861 ccaggaagtt catgaacagc ttcgccccgt tcgggctgtg cgagcccttc agaatggcag
     1921 caggcgaata atcaaccagg gagaacgact tggcgtagtt caccgccagc gggttgccgg
     1981 ccgccatgct gcggaacgcc gaaccatcgg cgcagatcgc cacatgccgt tcaccggagt
     2041 tcagcaccgc cactgcgtca tcgatcgagc ggccgatcag cggattgagc ttgttcagct
     2101 tctggaagta gtcccagccg tatttctcgt tcatcgccac ggtccacacg ccgaccaggc
     2161 cgctgaagtt cgggctgccg aaagcgatgc ggttcttcca cttcggatcg gtcaggtcgg
     2221 tccagtcctt cggcgcttcc tccggcttca cgtgcttcgt gttgtacatg atggcgacga
     2281 ggccgagcca ggcgacatga tagtagccgc cgccgttgaa cttgcgcggg ccttcgacca
     2341 gcgccgaatc gttcggcggc gtgtagttca ccagcttgcc cttgctgata aggtagctca
     2401 tgtggctggc atcggtgctg ctgaacacgt cagcctgcac cgcgccggcg ttgatgtcct
     2461 gcatcacgcg ctggaaggcg acctgggatg tggtcttggt ggcatccacc tggatgccgg
     2521 gatacttggc ggtgaaggcc ttgccgacgg attcaacgat cggctgcgtc agaaacccgg
     2581 agtaccaggt gacgcggcct tctttcttcg ccgcggcata gagttgctgc tcgtcatcgg
     2641 ccgcatgggc ggccgacagg ccaagggaag ttgcgccaag cgcgatcatc gccgctcgcc
     2701 tggtaaggct catggtccgt ttcctcgtct tgggccaagc ggcccgattg gtcgtgttgc
     2761 gtgctacgtg atgcgcagcc agattgttcc gactgcagcg tcgtgacgcg agagtaactc
     2821 tagcaggcgg cgccggtcgc gcaaccggcc ttcagcaatt ttctccatcc tccgaccggc
     2881 cgcaccgggt ggacgcacac cgccgcttga ttgacctgtc ctggcagatc gtccagcgtt
     2941 cgctccccgt atgttgggag gaaacgtaat ggccaaagtg aacggcgcga cagtgatggc
     3001 gcgcagcctg gcgcagcagg gcgtgaaatt catgttcgga gtcgtgggtt ttcccgtcgg
     3061 cccgattgcg gaagccgcgc agaaggccgg catcacctat ctcggcatgc gcaacgaaca
     3121 gtcggcatcc tacgcggcgc aggcgatcgg ctacctgacc ggccgtcccg gcgcctgtct
     3181 ggtggtgtcg ggacccggcg tggtgcacgg gctggccgga ctggcgaatg cgcagcagaa
     3241 ctgctggccg atgatcctga tcggcggcgc ttcacccacc taccagaacg gcatgggcgc
     3301 gttccaggag gagcggcagg tgcagatcgc cagcccgttc tgcaagttcg cgcatgcggt
     3361 ggagcacgtg caccgcattc cgttctacgt ggaaatggcc gtgcggaacg cgatctatgg
     3421 ccggcccggc gcctgctacc tggatatgcc ggatgacatc atccagggcc aggtggagga
     3481 ggagcaggtg ctccaggccg ccacggtggc cgaaccgccg cgtttccagg cgatgccgga
     3541 ggatatcgag aaggcgctga acacgctgga atccgccgag cggccgctgg tgatcgtcgg
     3601 caagggcatg ggctggtcgc gcgcggaaga cgaagtgcgc aagttcatcg atcgcacaca
     3661 gttgccgttc ctcgcctcgc cgatgggcaa gggcgtgatg gacgacacgc atccgctgtc
     3721 ggtcggcgcc gcgcgcagtc acgctctgca ggaagccgac gtcatcttcc tgatgggcgc
     3781 tcggctgaac tggatcatgc atttcggcgc gccgccgcgc ttcaacaaga atgtgcgcat
     3841 cgtccaactg gacatcgccg ccgaggcgat cagccagaac gtgccggccg aagtggccct
     3901 ggtgggcgac ggcaaggcca tcatcgggca attgaacaag gcattggaga cccgtcagtg
     3961 gttctacccg aaggacacgc cgtggcataa ggcgatcgcc gagaaagcgg cggccaacgc
     4021 caagcagatc cagccgatga tcgacgacaa cagcgcgccg accaactact accgcgcgtt
     4081 caaggacatc agcgcgtggc tgccggatga cgcggtgatc atcggcgagg gcgccaacac
     4141 gatggacatc ggccgcacgc agatggcgaa caagaatgcg cgccttcggc tggatgcggg
     4201 cacctacggc acgatgggta tcggcatggg cttcaccatc gcggccgcga tggcgcaccc
     4261 ggagcggccg atcgtgtcgg tgttcggcga cgcggcgctg ggcttctccg gcatggagat
     4321 cgaaaccgcc tgccgctacc ggctgccggt gaagatcgtc gtgctgaaca atggcggcat
     4381 cggcagcggc atggcggaat tcccggaaga ccgcagcacg ctgccgccgc gggtgatggc
     4441 gatcggctcg ggctacgaga agatcatgga tgccttcggc ggcaagggct tctacgtgga
     4501 cgatccgaag gacctgcgcg ccgcgctgga cgcggccatg gcatatgacg ggccggcgct
     4561 ggtgaacgtg aagctgcacc atgcggcagg ccgcaagccg cagcagttcc actggcacac
     4621 cgcgtaagcc gagggcgggc ggggagggct gctgcagccc tctccgcggc cgggtccggc
     4681 ccggctacga caccagactt acaggtagta ttccgccagc ggcgcgaagc cgttgaactt
     4741 ctggctggca taggtggtca cgtacgcgcc ggtggacagc agttccaccc ggtcgccgga
     4801 ttccagcgcc agcggcaggc ggtagttcgc cttctcgtac atgatgtccg cgccgtcgca
     4861 ggtcgggccg gcgatcgcca ccggccccat ggcggtgccg tcgtgcttgg tgacgatgcg
     4921 gtacttgatc gactcgccct cggtctccgc cagcccgccg aaccggccga tgtccaggta
     4981 cacccagcgg accgggctgt ccggatcgcg ccggctcacc agcaccacct ccgccgatac
     5041 cagcccggca tcgccgacga tgaaacggcc cggctcgatc agcatctccg gcagcgcgtt
     5101 gccgaaatgc ccggtcatcg cggtcatgat cgcgctgccg aactggtcga tctccggcac
     5161 ctcgtcgcgg taacgcgtgg ggaagccgcc gcccaggttc atcatgcgca ggttcacgcc
     5221 ggcttcggtc aggtcggtga acagcatcgc caccttgccg atcgccatct cataggcgcg
     5281 ggtcgtggtc tgctggctgc cgacatggaa cgacaggccg tacggatcca gccccatctc
     5341 gcccgcacgc agcatcagcg tccgcgcatt gtcgatcgtg gtgccgaact tgcgcgacag
     5401 cggccagtcg gcgccttcgt tctccaccag gatgcggcag taaacgcgcg ctcccggggc
     5461 ctgctgcgcc agcttctgca gctcctcgat cgaatcgaag gcgaacagcg acaccccgcg
     5521 ctcatgcgcg gcgcgaatcg ccgaggcctt cttgatggtg tttccgaagc tgatcgcctc
     5581 cggccgagcg ccggcatcca ggcaggcgtg gacttcctcg atgctggcgg catcgaagct
     5641 gctggacagg cgcaccaagc gctccagcac ctgcggcgcc ggattcgcct ttacggcgta
     5701 gtagatgcgc gccagcggca gcgcctgttg cagcgcgcgg aaattcgctt ccacgcgatc
     5761 catgtccagc accaggcacg gcgtggccgg ctgctgctgc gcaaggaatc gggcgatttt
     5821 gggtgtcatc gcaccgggcc cccctaccaa agcgcccgcc tggaccgagg cgacgggcgg
     5881 agttgacgaa acggtcgaac gaaccagcga ccagagcgat tgccagaacg cttgacgtcg
//
LOCUS       DASSZH010000470         4952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_113512, whole genome shotgun sequence.
ACCESSION   DASSZH010000470 DASSZH010000000
VERSION     DASSZH010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4952
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113512"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            442..1488
                     /locus_tag="VFN42_10185"
     CDS             442..1488
                     /locus_tag="VFN42_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997023.1"
                     /translation="MPVPDIFASFADPVPVTSSAPLCAVLIDAEEDFDWLRPVRGVAY
                     DVTCMRHLSDFQDILAAYRAVPTYLLTYPVLQDAGIVADLRRRLERGQCQLGVQLHPW
                     VTPPFSEAAEVRNSFTGNLQGPLEEQKLLELKRLFRACFGADPVIFRAGRYGLGMHTP
                     ALLEQHGFLIDTSLAPCSSFTDEAGPDFSASDYRTFWFGRSRRLLELPLCRSIVGWGG
                     RPAARAYGRLAAGAPPGGIAPAYLLGLLAWTRCAERITLSPEGNDATAVGRLIGSLLR
                     RGQNLLSLSLHSTSLSIGRNPYVRSRIELHLFYDRLSAILDMLTGRFGVRFVTASDLP
                     RMLSPQDVRLPDAA"
     gene            1478..2767
                     /locus_tag="VFN42_10190"
     CDS             1478..2767
                     /locus_tag="VFN42_10190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET8997024.1"
                     /translation="MPHDRGGDEGRRRILLITNNLPPVRGGSANVYDNLARHAGGDIA
                     VIAPKLSYLDGLPLIAWREHDRLAPYPVIRLNLLRTIIDPARPGGFLHRGLPRLWLRL
                     HDLAIRVRLLAVLAHALMRERVAGVCIGELIASAWLLDVLRVCFPRVTRAVYVHGEEI
                     TTSFGYDTTLERRRRALLAAQHVVVVSRFTAGVVAELLGASAPGRIRLIENGVDVARF
                     RPLPRPADLVAKYGLGGCFVFISVCRLLEKKGIDHAILAFAEIAAAHPDSRYLVIGDG
                     PYGPALAAIAAERGVADKVVFTGAVADDELVEHYALGDIFVMPNRRLDNGDTEGFGLV
                     FLEANACGLPVIAGSDGGSKDAVRHGVNGLLVDGHSVSAIAAAMRDLRQDAALRERLR
                     AGGQAVAAGADWRHKAREFMALLLPEQPAPGPGFMES"
     gene            complement(2865..3008)
                     /locus_tag="VFN42_10195"
                     /pseudo
     CDS             complement(2865..3008)
                     /locus_tag="VFN42_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010875092.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            3232..4707
                     /locus_tag="VFN42_10200"
     CDS             3232..4707
                     /locus_tag="VFN42_10200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HET8997025.1"
                     /translation="MTLLTSDPATAEQAAASLAAAPAFIRRRQERNFHPVTCGLALFA
                     TDCLAVSVATLLAAYGAGFFTHGDVMPARAPGLPIPLELAALFTTLIVYFTLKGRYGE
                     RIPFWTETHLVVSSSIFAAATEAVFSLLTGRGFEDRWAVAALLAFPVLAVPANRLVKH
                     VLACAGLWMLPVVVIGDGPTVLEAEAALGSDRSLGYCFVGRIDPAVLMAEPATTRLRA
                     VLARYRSEKVLIAIDSDGCLQRQLIECALRERVPFAVVPPPRALPAFDWNSTRLFSHD
                     TTLLSFHDGLSRPMLRIVKAAIDVSAALVLLLLTSPLFFVLATAICLDGGPVFYAHRR
                     VGTGGRPFLCLKFRTMVTNGDRILQELLSKDTALALEWSQTRKLVRDPRVTRIGRFLR
                     RTSLDELPQLINVLRLEMSLVGPRPIVESEIPLYGEYIAHYYATRPGITGLWQVSGRS
                     NTSYARRVQLDVWYVNNWSVWNDMAVLLKTVPAVLGRQGAH"
     gene            4715..>4952
                     /locus_tag="VFN42_10205"
     CDS             4715..>4952
                     /locus_tag="VFN42_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8997026.1"
                     /translation="MMRQSEDMAECPSAAGATIPMRIAVGIATRGRPEILRETLRELE
                     RQTHPPARIVVSHVVWTDVAGSGSPVPGLPQIEFL"
BASE COUNT          725 a         1692 c         1635 g          900 t
ORIGIN      
        1 aaactttccc gagacactaa aatggaattg ttgcttgcag aacagagaca atgtgcagcc
       61 ggccctcgcg gacgatcggg tcgcagtatc cacgcgcctg cgtaccggca ggcagcgaca
      121 gcgcagaccc gacaatatgt ctcgtggaca gcatcattgc ttacatgtca ctctcgcacc
      181 ttcctctgcc gcccgccgag cccaccgagc gttagcgcac gttgacgcaa agcgcaacga
      241 tccgcgaggc cgcttgcagc tgggttgcca ccttgcaacc ggcgccgtcc atcgcattcc
      301 cggcgggatg gcggctctgt cgtccttggg acgtatccgt tgcggcataa caatatctgg
      361 cgcagagcga caccgccccc tcccgcgctg gcgcaggcgg ccgggcttct gatacacggg
      421 actgcccccg caacggctcc ggtgcccgtg cccgacatct tcgccagctt cgccgatccc
      481 gtccccgtca cgtcgtctgc gccactgtgc gccgtcctga tcgacgccga ggaggatttc
      541 gactggctgc ggccggtgcg cggggtggcg tacgacgtga cctgcatgcg gcatctgtcg
      601 gatttccagg acatccttgc cgcctaccgc gccgtgccga cctatcttct gacctatccg
      661 gtgctgcagg atgccggcat cgtcgccgac ctgcgccggc ggctggagcg gggacagtgc
      721 cagctcggcg tccagctgca tccctgggtg acgccgccgt tcagcgaggc ggcggaggtg
      781 cgcaactcgt ttaccggcaa cctccagggc ccgctcgagg aacagaagct gctcgaactg
      841 aagcggctgt ttcgcgcctg cttcggcgcg gacccggtca tcttccgcgc cggccgctac
      901 ggactgggca tgcacacgcc ggccctgctc gaacagcacg ggttcctgat cgacaccagc
      961 ctcgccccct gcagctcgtt tactgacgag gcagggccgg atttctccgc cagcgactat
     1021 cgcaccttct ggttcggccg ctcccgccgg ctgctggagc tgccgttgtg ccgcagcatc
     1081 gtcggctggg gcggtcggcc ggccgcgcgt gcctacgggc ggcttgccgc cggggcgccg
     1141 cccggtggca ttgccccggc ctatctgctc ggtctgctgg cctggacccg ctgcgccgag
     1201 cgcatcaccc tctcgccgga gggcaacgac gccaccgccg tcgggcggct gatcggctct
     1261 ctcctgcggc gcggacagaa cctgttgtcg ctgagcctgc acagcacgtc gctcagcatc
     1321 gggcggaacc cctatgtgcg cagccggatc gagttgcacc tgttctacga ccggctctcc
     1381 gccattctcg acatgctgac cggccgcttc ggcgtgcggt tcgtcacggc atcggacctg
     1441 ccgcgcatgc tgtcacccca ggacgtgcgg ctgcccgatg ccgcatgaca gaggcgggga
     1501 tgagggccgc cgtcgcatcc tgctgatcac caacaacctg ccgccggtgc gcggcgggtc
     1561 ggccaatgtc tatgacaatc tggcccgcca tgccggcggc gacattgcgg tgatcgcgcc
     1621 gaagctgagc tatctcgacg gcctgccgct gatcgcctgg cgggagcatg accggctggc
     1681 gccgtacccg gtgatccggc tgaacctgct gcgcacgatc atagaccccg cccgccccgg
     1741 cgggttcctg caccgcgggc tgccccggct gtggttgcgc ctgcacgacc tggccatccg
     1801 cgtccggctg ctggccgtac tggcgcacgc cctgatgcgc gagcgcgtcg cgggggtctg
     1861 tatcggcgag ctgattgcca gtgcctggct gcttgacgtg ctgcgcgtct gcttcccacg
     1921 ggtgacccgc gccgtctatg tgcatggcga ggaaatcacg acctccttcg gctacgacac
     1981 gacgctggag cgccgccgcc gggccttgct ggccgcgcag cacgtcgtcg tggtcagtcg
     2041 cttcaccgct ggcgtggtcg ccgaattgct gggcgcgtcc gcgcccggac gcattcggct
     2101 gatcgagaac ggcgtggacg ttgcccgttt tcgcccgctg ccgcgcccgg ccgatctggt
     2161 ggcgaaatac gggttgggcg gctgcttcgt gttcatctcg gtctgccggc tgctggaaaa
     2221 gaagggcatc gatcatgcca ttctcgcctt cgccgagatc gcggccgcgc atcccgacag
     2281 ccgttacctg gtgatcggtg acggtccgta cggcccggca ctggccgcca tcgcggcgga
     2341 gcgcggcgtg gcggacaagg tggtgttcac gggcgccgtc gccgatgacg aactggtgga
     2401 acactacgcg ctcggcgata tattcgtgat gcccaaccgc cgcctcgaca acggcgacac
     2461 cgagggcttt ggcctcgtgt tcctggaggc caatgcctgc ggcctgccgg tgatcgccgg
     2521 cagtgacggg ggcagcaagg atgccgtgcg ccacggcgtc aacggcctgc tggtggacgg
     2581 ccattcggtc agcgcgattg ccgccgcgat gcgggacctg cgccaggacg cggcattgcg
     2641 cgaacgcctg cgcgccggcg gccaggccgt cgccgccggc gccgactggc ggcacaaggc
     2701 ccgggagttc atggctctgc tccttccgga acagcccgcg cctggccccg gattcatgga
     2761 gagctgacct ccccggagcg tgcggtggag gcagtgcgcc gcccggctga tcgtcgcgcg
     2821 gtgggcctgc atggctgtcc gggtggggca tgatttcgtg ctctggccgg attgacggag
     2881 ggtggtcgtt cgatggtgcc gaggtcgcgg cgctcgaaca ggcagcttgc gacgagttcg
     2941 gtgaagcgca ggacaggtat tctgacgcga tgcctgcggg cggcgagacg cagcagggcg
     3001 taggcaaccc aatccccgcc tgtcacccgc tgtcacacga gttcggcgcc ctgggcactg
     3061 ggtgtaagca attgccttcg ttccggaatc ccgttcgcct ctgcggcggc acgcacgctg
     3121 acctttgtgg gtacggctgc ctgacggata cgactgaaag acaacaggcg ctccggttcc
     3181 gacaggcata tacgccaatg ctctcgaaat tgaagcgggt tggagggcgg cttgacctta
     3241 ctgaccagcg atccagctac ggcggaacaa gcggcagcat ccctggcggc ggcaccggcg
     3301 ttcatccgtc gcaggcagga gcgcaacttt catcccgtga cctgcgggct ggcattgttt
     3361 gccaccgatt gcctcgccgt gtccgtcgcg acattgctgg ccgcctacgg cgccggtttc
     3421 ttcacgcatg gcgacgtcat gcccgcgcgc gctcccggat taccgatccc gcttgaactc
     3481 gccgcgctgt tcacgaccct catcgtctat ttcacgctga aagggcgcta tggggagcgg
     3541 attccatttt ggaccgaaac gcaccttgtc gtttcctcca gcatcttcgc cgccgctacc
     3601 gaagcggtgt tcagcctgct gacgggccgc ggtttcgaag accggtgggc agtggcagcc
     3661 ctgctggctt ttcctgtcct ggcggtcccc gcgaaccggc tggtcaagca cgtcctcgcc
     3721 tgcgcgggcc tgtggatgct gccggtcgtc gtcatcggcg atggccccac cgtgctggag
     3781 gcagaggccg ccctgggatc tgatcgttcg ctcggctact gcttcgtcgg tcggatcgac
     3841 ccggccgtcc tgatggcgga gcccgcgacg acgcggttgc gcgcggtact ggcccgctat
     3901 cgctcggaaa aggtgctgat tgcgattgac agtgatggat gcctgcaacg gcaactcatc
     3961 gaatgcgcct tgcgggaaag ggttccgttc gcggtggttc ccccgccgcg cgccctcccc
     4021 gcattcgact ggaattcaac gcgcctgttc agccacgaca ccacgttgct gtcgttccac
     4081 gacgggctgt cccggccgat gctgcgcatc gtcaaggcgg cgatcgacgt ttcggcggcc
     4141 ctggtgctgc tgttactgac cagcccgctc ttcttcgtgc ttgcaaccgc catctgcctg
     4201 gacggcggcc cggtcttcta cgcgcaccgg cgcgtgggca ccggcggacg tccgttcctg
     4261 tgcctcaagt tccgaacgat ggtgaccaac ggggaccgca tccttcagga actgctgagc
     4321 aaggacaccg cccttgccct ggaatggtcg cagacgcgca agctcgtgcg cgacccgcgc
     4381 gtgaccagga tcgggcgatt cctgcggcgg accagcctcg acgaactgcc ccaactcatc
     4441 aacgtgctgc gcctggagat gagcctggtt ggtcctcggc cgatcgtgga gagcgagatc
     4501 ccgctgtacg gcgagtatat cgcgcattac tatgcaactc ggccggggat cacgggcctc
     4561 tggcaggtca gcggccgcag caacacgtcc tatgcgcggc gtgtccagct ggatgtctgg
     4621 tacgtcaaca actggtccgt ttggaacgac atggcggtgt tgctgaagac cgtaccggcc
     4681 gtgctgggcc ggcagggcgc ccattgatcg cctgatgatg cggcagtccg aagacatggc
     4741 ggaatgtcca tccgcagccg gcgcgacgat accgatgcgg atcgcggtcg gaattgccac
     4801 ccggggacgt cccgaaatcc tcagggagac gctgcgcgaa ctggaacggc agacccaccc
     4861 gcccgcgcgg atcgttgtct cccatgtcgt atggacggat gtcgccggta gcggcagccc
     4921 cgtaccgggc ctgccgcaga tcgagttcct gg
//
LOCUS       DASSZH010000471         4711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1460451, whole genome shotgun sequence.
ACCESSION   DASSZH010000471 DASSZH010000000
VERSION     DASSZH010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460451"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VFN42_10210"
     CDS             complement(<1..278)
                     /locus_tag="VFN42_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HET8997027.1"
                     /translation="MTRSIGEIELQIMWNRLLSVVEEQAQTLVRTAFSTSAREAGDIS
                     AGVFDLQGRMLAQAVTGTPGHINTMARSVGHFLDEFPVAQMQPGDVYVT"
     gene            complement(275..2344)
                     /locus_tag="VFN42_10215"
     CDS             complement(275..2344)
                     /locus_tag="VFN42_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HET8997028.1"
                     /translation="MPSPTRLAVDIGGTFTDLVLALPDRTLSAKLLTTPDSPDAAVIA
                     GTRAILAEAGIEAAQLDLVIHGTTLATNALIERKGARTALITTRGFRDSLEIAYEHRF
                     EQYDLYMERPAPLVERDLRLEVPERLAADGSLLLALDEAALEALVPVLRSHAIEAVAV
                     SFLHSYVNPAHEERARVLLARAMPELAITISSDVCREIREYERTSTTVANAYVLPLMD
                     RYLARMQDGLRALGAACPLLLMMSSGGICTVETARRFPIRLVESGPAGGAILARHVAA
                     RGGYDRAISFDMGGTTAKITLIDDFAPQQSRSFEIARTARFAKGSGFPVRIPVIDMVE
                     IGAGGGSIARVDALRRIVVGPDSAGSAPGPACYARGGTAPTVTDADVTLGRIDPAQFA
                     GGKIALDVASAERAIAETMATPLDMPLTEAARGIAEIVDETMASAARVHAVENGKDTG
                     ARTLIAFGGAAPLHAARLAQKLGMKRVVIPPDAGVGSAFGFLEAPVAYEVVRTRLLRL
                     DALDQHEIDALFAGMRAEAASVVRLAVPHGALQETRTAYMRYRGQGHEIAVPLGDGAL
                     DPETLRAAFDATYKLLFGRVIPRLEIEALTWTLSLAQPHALPPRAADPADAGAARPTG
                     TRRIVEPGIGAAVEAAVFTRDTLQPGATLHGPAVIVEAGTSTIVPTGFTARIAAGREI
                     VIEESAA"
     gene            complement(2446..3765)
                     /locus_tag="VFN42_10220"
     CDS             complement(2446..3765)
                     /locus_tag="VFN42_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997029.1"
                     /translation="MNRITRSRCLAAGMWSGVLLGCVLGFLLIVLCLPAETLPRSAQS
                     GERWLLLAALVTLGTCLVAAPLIRYLDAGWHCRLDEFRNRIRDGAVLCYLAQFWEKRL
                     DGRPQARGDRKAAEALFDEIYVGYNGRRAFAAPAILLLALSFISIALMAQSGIDACMA
                     HRCVIADHEDGRAAVSLMTELQPTERFAPVGDIVLPLASAAALGGAFLFVIGDAILRA
                     RRCTTNVSDLYWYALRIALAIPMGLAFTELAARNVGALVAFGLGAFPIEALTKLLRRY
                     TNKSIGGSAEEAQETDQLIRLDGVTVPIAAALAAEGVDSIDELVGVDPVLLAFRSGIP
                     FPSVLRFASQAVVHIHLDHASADLVRIGLGNAYLVSQLVMALDEGRKPGNDPKPAEQR
                     LDDAVAVLNAAARRNDQAAPPQSAASVEAAFRNIMHHGYTQFLLSVS"
     gene            3921..4427
                     /locus_tag="VFN42_10225"
     CDS             3921..4427
                     /locus_tag="VFN42_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010514113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997030.1"
                     /translation="MSDQEFAASDFLNQVIRPALTAIGQDSPAAEQLLLATALQESDL
                     RTTVQMGGGPALGYFQMEPATHDDIWANFLRYRAELASGLRRLAGIAAGVPHAGLLAS
                     NHRYAAAMTRVHYMRVPAPLPAAGDAAAMAAYWKDHYNTAGGAGSTAQFLRKWANHRV
                     AALVAELV"
     gene            complement(4462..>4711)
                     /locus_tag="VFN42_10230"
     CDS             complement(4462..>4711)
                     /locus_tag="VFN42_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997031.1"
                     /translation="VCAEAIAFGRAVLEGDGCIETAVAVRHPKPDELDRELAVVPPCG
                     ACREMILDHCPEALVIVKEAGVLLKRPIRSLLPVPYRR"
BASE COUNT          752 a         1603 c         1642 g          714 t
ORIGIN      
        1 gtgacgtaca cgtcgcccgg ctgcatctgc gccaccggga attcgtccag gaagtgcccg
       61 acggaccgcg ccatggtgtt gatgtggcct ggcgtgccgg tgaccgcctg cgccagcatg
      121 cgtccctgca ggtcgaacac gccggcggag atgtcgccgg cctcgcgcgc cgaggtgctg
      181 aacgcggtac gcaccagggt ctgcgcctgc tcctccacca ccgacagcag gcggttccac
      241 atgatctgca gttcgatttc accgatgctg cgtgtcatgc ggcggactcc tcgatgacga
      301 tttcgcgtcc ggcggcgatg cgcgcggtga atccagtcgg aacgatggtg gaggtgccgg
      361 cctcgacgat cactgccggt ccgtgcagcg tggcgcccgg ctgcaatgtg tcgcgcgtga
      421 acaccgcggc ctccaccgcc gcgccgatgc cgggttccac gatgcgccgc gtgccggtcg
      481 gccgcgccgc accagcgtcg gccgggtcgg cggcgcgcgg cggcagggcg tgcggctgcg
      541 ccaaggacag ggtccaggtc agcgcttcga tctccagccg cggaatgacg cggccgaaca
      601 gcagcttgta ggtggcatcg aacgcggcgc gcagggtctc cggatccagc gcgccatcgc
      661 ccagcggcac ggcaatctcg tggccctggc cgcgatagcg catgtaggcg gtgcgggttt
      721 cctgcaaggc gccgtgcggc accgcgaggc gcaccaccga tgctgcctcg gcgcgcatcc
      781 cggcgaacag cgcgtcgatc tcgtgctggt cgagcgcgtc caggcgcagc aggcgcgtgc
      841 gcaccacttc atacgccacc ggcgcttcca ggaagccgaa cgcggagccc acgccggcat
      901 ccggcggaat caccacgcgc ttcatcccga gcttctgcgc cagccgcgcg gcgtgcagcg
      961 gggcagcgcc gccgaaggcg atcagcgtgc gcgcgccggt atccttgccg ttctccaccg
     1021 catgcacgcg cgcggcgctg gccatggttt catcgacgat ttccgcaatc ccgcgcgccg
     1081 cttcggtcag cggcatgtcc agcggcgtgg ccatcgtctc tgctatggcg cgttcggcag
     1141 acgcgacatc cagcgcgatc ttgccgccgg cgaattgcgc cggatcgatg cggccgagcg
     1201 tcacgtcggc atcggtgacg gtcggtgcgg tgccgccgcg cgcgtagcag gccgggccgg
     1261 gggcggagcc ggcgctgtcc gggccgacca cgatgcgccg cagcgcatcc acccgggcga
     1321 tggagccgcc gccggcgccg atttccacca tgtcgatcac cgggatgcgc accggaaagc
     1381 cgctgccctt ggcgaaccgg gcagtacggg caatttcgaa actgcgcgat tgctgcggcg
     1441 cgaagtcgtc gatcagcgtg atcttcgcgg tcgtaccgcc catgtcgaac gagatcgccc
     1501 gatcgtagcc gccgcgcgcc gcgacatgcc gcgcgagaat ggcaccgccg gccggaccgc
     1561 tttccaccag gcggatcgga aaccggcgcg cggtctccac cgtgcagatg ccgccggacg
     1621 acatcatcag cagcaacgga cacgcggcgc cgagcgcgcg cagcccgtcc tgcatgcgcg
     1681 ccagatagcg gtccatcagc ggcagcacgt aggcattggc gaccgtggtg gaggtgcgct
     1741 cgtactcgcg gatctcgcgg catacgtcgg aggaaatcgt gatggccagc tccggcatcg
     1801 cgcgcgccag caggacgcgg gcgcgttcct catgtgccgg attgacgtag gaatgcagga
     1861 agctgactgc cacggcttcg atggcgtgag accgcagcac cggcaccagc gcttcaagcg
     1921 ccgcctcgtc cagcgccagc agcagcgagc cgtcggcggc gagacgttcc ggtacttcca
     1981 gccgcagatc gcgttccacc agcggcgcgg gccgctccat gtacaggtcg tattgttcga
     2041 agcggtgctc gtaggcgatc tccagcgaat cgcgaaaacc acgcgtggtg atcagcgcgg
     2101 tgcgcgcgcc cttgcgttcg attagcgcat tggtggcgag cgtggtgccg tggatcacca
     2161 ggtcgagctg cgccgcttcg atgccggcct cggccaggat cgcgcgtgtg ccggcgatca
     2221 ccgccgcgtc gggcgaatcg ggcgtggtca gcagcttggc cgacagcgtg cggtccggca
     2281 gggcgagcac gaggtcggtg aaggtgccgc cgatatcgac ggcgaggcgg gtcggcgagg
     2341 gcatggggca gacaaccgcg gcagggtggg ccggcagtgt ggcaccgggc cggcgaacgc
     2401 gccaatctcc tgcggcgccg cagccggcgg tgcctttagg tgcgctcagc tgacgctgag
     2461 caggaattgt gtgtagccgt ggtgcatgat gttgcggaac gccgcctcga cgctggcggc
     2521 tgactgcggc ggggccgctt ggtcattgcg cctcgccgcg gcgttcagca ccgctaccgc
     2581 gtcgtccagc cgctgctccg ccggctttgg gtcgttgccc ggcttgcggc cctcatccag
     2641 agccatgacg agctgactga ccaggtacgc attgcccagg ccgatccgca ccagatcggc
     2701 ggacgcatgg tccaggtgga tgtgcaccac cgcttgcgag gcaaagcgca gcaccgaggg
     2761 aaacggaatg ccgctgcgga acgccagcag caccggatcg acgccaacca gctcgtcgat
     2821 cgaatcgacc ccctcggccg cgagtgccgc ggcgattggc acggtgacgc catcgagccg
     2881 gatcaactgg tcggtttcct gtgcctcctc ggccgaaccg ccgatcgact tgttggtgta
     2941 gcgccgcagc agcttggtca gcgcttcgat gggaaacgct cccaggccga acgccaccag
     3001 cgcgccgaca ttgcgggccg cgagttcggt gaaggccagt cccatgggaa tcgcgagtgc
     3061 gatccgcagc gcgtaccagt acagatcgga gacgttggtg gtacagcgtc gtgctcgcag
     3121 gatcgcgtcg ccgatgacga acaggaaggc gccacccagc gccgcggcgg aagccagcgg
     3181 caaaacgata tcgccgaccg gggcaaagcg ctccgtcggc tgcagctcgg tcatcagcga
     3241 cacggcggct ctgccgtctt catggtcggc aatgacgcag cgatgcgcca tgcaggcgtc
     3301 gatgccggac tgcgccatca gggcgatgct gatgaagctg agcgccagca gcaggatggc
     3361 cggggcggcg aaggcgcgcc ggccgttgta gccgacatag atctcgtcaa acagcgcctc
     3421 ggctgccttg cggtcgccgc gtgcctgtgg ccggccgtcg agccgctttt cccagaactg
     3481 cgcgaggtag cacagcaccg cgccgtcacg gatgcggttg cggaactcgt ccagccggca
     3541 atgccagccg gcatcgaggt agcggatcag cggcgcggct accaggcagg tgcccagtgt
     3601 caccagtgcg gccagcagca accagcgttc gccgctctgc gccgatcgcg gcagggtttc
     3661 cgccggcagg cagagcacaa tgagcaggaa gcccagcaca cagccgagca ggacgccgct
     3721 ccacatgccg gcggcgaggc agcgagacct ggtgatgcgg ttcacgttcg cagtctccgt
     3781 gtggcggccg ttcgccggtg tggcggggcg gcgtgcctgc ggtcatctct gggcagcaat
     3841 taatgcatat atcaaattgt gacgtcgttt gctatagttg ctttgtcggt ccgggcgaga
     3901 cgcgcagaag gagcttggct gtgtcggatc aggaatttgc cgcgtctgac ttcctgaatc
     3961 aggtgatccg tccggcattg accgcgatcg gacaggattc gccggcggcg gaacagctgc
     4021 tgctggccac cgcgctgcag gaaagcgacc tgcgcaccac ggtgcagatg ggcggcgggc
     4081 cggcgctcgg ctatttccag atggagcccg cgacgcacga cgacatctgg gccaatttcc
     4141 tgcgctaccg cgcggagctg gccagcgggc tgcggcgtct ggccggcatt gccgcggggg
     4201 ttccgcacgc cggactgctt gccagcaacc accgttacgc ggcagccatg acgcgcgtcc
     4261 actatatgcg ggtgcctgcg ccgctaccgg cggcaggcga tgccgcggcg atggccgcgt
     4321 actggaagga ccactacaat accgcgggcg gcgcaggcag caccgcgcag ttcctgcgga
     4381 aatgggcgaa ccatcgggtc gccgcgctgg tcgccgagct ggtctagacc cgcaccacgc
     4441 tcgtggcgga gcttccgaca ctcatcgccg atagggtacc ggcagcagcg aacggatcgg
     4501 ccgcttcagc agcacgcccg cttccttcac gatgaccagc gcctccggac aatggtccag
     4561 aatcatctcc cggcacgcac cgcagggcgg caccaccgcc agttcgcggt ccaactcgtc
     4621 cggcttcgga tggcgtaccg ccacggccgt ctcgatgcag ccatcgccct ccagcaccgc
     4681 tcggccgaac gcgatcgcct cggcgcagac c
//
LOCUS       DASSZH010000472         5326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_96512, whole genome shotgun sequence.
ACCESSION   DASSZH010000472 DASSZH010000000
VERSION     DASSZH010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96512"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..915)
                     /locus_tag="VFN42_10235"
     CDS             complement(139..915)
                     /locus_tag="VFN42_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8997032.1"
                     /translation="MDPVTPKIRLRNLTKAFGSKVVLDGIDLDVMPRTSMVVIGGSGS
                     GKSVLLKCILGLIEPDDGIIEIDGRDILHLPRAEREAMRARIGMLFQNGALFDSLPVW
                     ENVSFGLLAQGKVTRRQARDHAAEFLAQVGLAPSVGDLSPAELSGGMQKRVALARAIA
                     AQPDIMFFDEPTTGLDPIMGAVIDGLIVDLVKHLGSTAVAITHDMASARRIGDNAAML
                     HRGKIVWSGPASELLESGNPVVDQFTHGRREGPIEMELRR"
     gene            1016..2956
                     /locus_tag="VFN42_10240"
     CDS             1016..2956
                     /locus_tag="VFN42_10240"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET8997033.1"
                     /translation="MRGSIRTTLMLLPLLAGAWQARAQDPNDPLPAIRANQWALAQTQ
                     AASYADPVAEKLVLFYRLLDPGAATPAEIAAFLHANPDWPDQLALKRRWQEAIAADPD
                     DQDVVQQCAAAHPSEPATLLRCAQALENTGDKSAAEVDARRAWITGITTKPEERAFLH
                     HWGDVITPAVQWTRFQHLSWYNSAAAARQMARLDPAYRRAAVAQLALQHDDAKAEQLL
                     AEVPAGLRDDPGLILDEARYLRRADRLGDAAALWRDSGAAAQAMAPDHRTAFWNERSL
                     LARKLLAQGDNQAAYDVVRDHGELPSAAMADAEFLTGFIALRRLHDPAAAMQDFRELA
                     RSHAAITEGRAEYWMGRAAAAAGADPKPYYEKAANWPTTFYGQLGALASGETPASLAR
                     RIDALRDPAWNRDTVLAFTGHEVVRAAAWLVAWGDPRRARAFLLRMDELAPSAAEQAL
                     TAQFALKLGVPEVAVFIARRMGFHGTLLAQAGWPMPYDPPADGLDPAVALGIMRQESS
                     FDTDAISPSGARGLMQLMPPTAAEVAKQLGVRTSLISLTADPAHNMQLGTSYLQAMLA
                     HFDGSLPLAVAAYNAGPHRVEQWLGVNGDPRSGAVDMLDWLELIPSGETRNYVQRVLE
                     NVVIYRARLGESAPTLLAEWTQ"
     gene            3071..3157
                     /locus_tag="VFN42_10245"
     tRNA            3071..3157
                     /locus_tag="VFN42_10245"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3105..3107,aa:Leu,seq:cag)
     gene            3212..3865
                     /locus_tag="VFN42_10250"
     CDS             3212..3865
                     /locus_tag="VFN42_10250"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HET8997034.1"
                     /translation="MSATQFIPGGAIHLHKHDLPETVDLGPVVAVDTETMGLNPHRDR
                     LCLVQLSSGDGHAHLVQLLPERLGGHGFDCPNLKRLLADPDVVKLMHFARFDVAVLQH
                     SLGITVAPVCCTKIAAKLIRTFTDKHGLKDLCKELLGVELSKQQQTSDWGAVELSSEQ
                     LSYAASDVLHLHALWAKLEQLLVRENRRDLADACFAFLPARARLDLLGYEDPDIFRH"
     gene            4020..5003
                     /locus_tag="VFN42_10255"
     CDS             4020..5003
                     /locus_tag="VFN42_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002730238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HET8997035.1"
                     /translation="MTAAAATLPTDLEVARSVLATEAAGLRALAAGLDESFDRAVSLL
                     AEIPGRVVVSGMGKSGHVARKIAATFASTGTPALFVHPAEASHGDLGMVVGGDAVLAL
                     SNSGETVELADLISHASRFRLPLVGITAQPDSSLANAANVTLLLPPSAEACPMGLAPT
                     TSTTMQMALGDALAVALLTRRGFTAAEFRQFHPGGRLGVRLRRVRDVMHSGDAIPLAP
                     PDLAMDRALLRITEKHFGCLGVIDSDGRLTGILTDGDLRRAMGPDLLSRRVGEIMTRN
                     PRTIGPDALAAEALGLMNARERPITALFVVDSDGMPIGILHVHDLLRAGVA"
     gene            5000..>5326
                     /locus_tag="VFN42_10260"
     CDS             5000..>5326
                     /locus_tag="VFN42_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HET8997036.1"
                     /translation="MSARPGLVRSARPRTNVPLAAAARSRRLPSAGALARRRWLIRLT
                     KWLLPVFALVLLSLLALWPEIDHPTDEARLSFRQMGGNIGGARLTDARYHAVDKQGRP
                     YTVTAAV"
BASE COUNT          814 a         1839 c         1806 g          867 t
ORIGIN      
        1 tgcaatgcgg tgcggctttc atgcctttgg tgtcaacctt gctggtcatg cgcaagaccc
       61 gcgtggttgc aagcgagcac ggtgatgccg aagcgcggcg catcgcaggc tcggatggcg
      121 ggggcaggct ccggcggatc agcgccgcag ttccatttcg atcggtccct cgcggcggcc
      181 gtgggtgaat tgatccacca cggggttgcc gctctccagc aattctgaag ccgggccgct
      241 ccacacgatc ttgccccggt gcagcatcgc cgcattgtcg ccgatccggc gggcgctcgc
      301 catgtcatgg gtgatcgcca ccgcggtgct gccaaggtgc ttcaccagat cgacgatcag
      361 cccatcgatg accgcgccca tgatcgggtc caggccggtg gtcggctcat cgaagaacat
      421 gatgtccggc tgcgccgcga tcgcccgcgc cagggcgacg cgcttctgca tgccgccgga
      481 cagttcggcc ggcgacaggt cgccgacgct cggcgccagc ccgacctgtg ccaggaactc
      541 agcggcatgg tcccgcgcct gccggcgagt cactttgccc tgcgccagca ggccgaagct
      601 gacattttcc cagactggca ggctgtcgaa caacgcgccg ttctggaaca gcatgccgat
      661 ccgcgcccgc atggcttccc gctccgcacg cggcaggtgc aggatgtcgc ggccatcgat
      721 ctcgataatg ccgtcatccg gttcgatcag gcccaggatg catttcagca gcaccgattt
      781 gccgctgccg gagccgccaa tcaccaccat ggaggtgcgc ggcatcacgt ccaggtcgat
      841 gccatccagc accaccttgc tgccaaacgc ctttgtcagg ttgcgcaggc gtatcttggg
      901 tgtgacaggg tccatgcgcc gcttgatggg gcctgggcgg gatttcggca acccgggcag
      961 gttgtccttt gcaaagaagc tgcggccgtc agccggtacg ggctatgaag gcggcatgcg
     1021 tggttcgatt cgcacgacct tgatgctgct gcctctgctg gccggggcct ggcaggcccg
     1081 cgcgcaggac cccaacgacc cgctcccggc gatccgcgcc aatcaatggg cgctggcgca
     1141 gacccaggcg gccagctacg ccgatccagt ggcggagaag ctggtgttgt tctaccgctt
     1201 gctcgacccc ggcgcggcga cgccagccga gattgccgcc ttcctgcacg ccaaccccga
     1261 ctggccggat cagcttgcgt tgaagcgccg ctggcaggag gcgatcgccg ccgatccgga
     1321 tgatcaggat gtggtgcagc aatgcgccgc ggcgcatccc agcgaaccgg cgaccctgct
     1381 gcggtgcgcc caggcgctgg agaataccgg ggacaagtca gccgcggagg tggacgctcg
     1441 gcgggcctgg atcaccggca tcaccacgaa accggaagaa cgtgcctttc tccaccactg
     1501 gggggacgtg atcacgccgg cggtgcaatg gacccgcttc cagcacctgt cctggtacaa
     1561 ttccgccgca gccgctcggc agatggcgcg actcgatccg gcgtatcgca gggccgcggt
     1621 cgcccagctc gcattgcagc acgacgatgc gaaggcggag cagctgctgg ccgaggtgcc
     1681 tgccggcctg cgcgatgatc cgggtctgat cctggacgaa gcccgctatc tgcggcgggc
     1741 ggaccgcctg ggcgatgcag ctgccctctg gcgggactcc ggcgccgccg cccaggccat
     1801 ggcgccggat caccggaccg ccttctggaa cgaacgcagc ctgctggcac gtaagctgct
     1861 tgcccaggga gacaaccagg ccgcctatga tgtcgtgcgc gaccatggcg agctgccgtc
     1921 ggcggcaatg gcggacgccg agtttctgac cggattcatt gcgctgcgcc ggctgcacga
     1981 tccggctgca gcgatgcagg attttcggga gctggcgcgc tcccacgccg cgattaccga
     2041 aggccgggcg gaatactgga tgggccgcgc ggctgccgcc gccggcgccg atccgaaacc
     2101 ctactatgag aaagccgcga actggccgac caccttctat ggacaactcg gggccctggc
     2161 ttcgggggag acgccggcca gcctggcccg ccgcatcgat gcgctccgcg atccggcatg
     2221 gaaccgcgac actgtgctgg ccttcaccgg ccatgaggtg gtgcgcgccg cggcgtggct
     2281 ggtggcctgg ggcgacccgc gccgcgcccg cgccttcctg ctgcgcatgg acgagctggc
     2341 gccgagcgcg gcagaacaag cgctgaccgc gcagttcgcg ctgaagctgg gcgtgccgga
     2401 agtcgccgtg ttcatcgccc gccgcatggg attccacggc acattgctgg cgcaggccgg
     2461 ctggccgatg ccgtacgatc ctcccgccga tgggctggac ccggccgtgg cgctgggcat
     2521 catgcgccag gagagcagct tcgacacgga cgcgatcagc ccgtcgggtg cgcgggggct
     2581 gatgcaactg atgccgccga ccgccgccga ggtcgccaaa cagctcggcg tgcgtacctc
     2641 gctgatttcg ctcaccgccg acccggcgca caacatgcag ctcggcacca gctacctgca
     2701 ggcgatgctg gcgcatttcg atggctcgct gcccctggcg gttgcggcgt acaatgcagg
     2761 gccacaccgg gtggagcagt ggctgggtgt gaacggtgac ccgcgcagcg gtgcggtgga
     2821 catgctggac tggctggagc tgatcccctc gggcgaaacc cgcaactatg tccagcgggt
     2881 gctggagaac gtggtgatct accgcgcgcg gctgggggaa agcgccccca ccctgctggc
     2941 ggaatggacg cagtgagggt gcgatccgac ctgtgacgaa attcgctcac gcgccggttg
     3001 acgcgccgcc caccgggggc tacatcgcgc gccctaccgc tcacccgcgg cgtgccgcgg
     3061 gatgcccgat gcccaggtgg cggaattggt agacgcgcag gtttcaggta cctgtggccg
     3121 caaggtcgtg gaagttcgag tcttctcctg ggcaccaaag gcgggctggc agcggcagca
     3181 gcccgcgcca gcagcggcga cgagatcagc agtgagcgcg acccagttca tcccaggcgg
     3241 cgccatccac ctgcacaagc acgacctgcc ggagacggtg gacctgggcc ccgtggtggc
     3301 cgtggacacc gaaaccatgg gcctgaatcc gcatcgggac cgcctgtgcc tggtgcagct
     3361 ttcttccggc gacggtcatg ctcacctggt gcaattgctg ccggaacgcc tgggcggaca
     3421 cggcttcgac tgcccgaacc tgaaacgcct gctggccgat cccgacgtgg tcaagctgat
     3481 gcacttcgcc cgcttcgacg tcgcggtcct gcagcacagc ctgggcatca cggtggcccc
     3541 ggtctgctgc accaagatcg ccgccaagct gatccgcacc ttcaccgaca aacacggtct
     3601 caaggacctg tgcaaggaac tgctcggcgt cgagctatcg aagcagcagc agacctcgga
     3661 ttggggcgcg gtggagttga gcagcgaaca gttgtcctat gcggcttcgg acgtgctgca
     3721 cctgcacgcg ctctgggcga agctcgagca gttgctggtg cgggagaacc ggcgcgacct
     3781 ggcggacgcc tgcttcgctt tcctgccggc ccgggcccgc ttggacctgt tgggctacga
     3841 ggatcccgac attttccggc attgaggctg catggcgttc ctcacccgcg ccccgggcgg
     3901 ggaatgaaaa cggttcatgt cgcttgtaac atttcgagag gtcaccccgg ttgaccctgc
     3961 tgcccgggga ggcccataga acggggcatc cgcatgtgcc tcccagggaa agccccaaga
     4021 tgactgccgc cgccgccacg cttcccaccg acctggaggt ggcgcgttcc gtcctcgcca
     4081 ccgaggcggc gggactgcgc gcgctggccg ccggcctgga tgagagtttc gaccgcgctg
     4141 tctccctgct ggccgaaatc cccggccggg tggtggtgtc gggcatgggc aagtccggcc
     4201 atgtcgcccg caagatcgcc gcgacgttcg cttccaccgg cacaccggca ttgttcgtcc
     4261 acccggccga agcatcgcat ggcgacctgg gcatggtggt tggtggcgac gcggtgctcg
     4321 cgctttccaa ttccggcgag acggtggagc tcgccgacct catttcccac gccagccgct
     4381 tccgcttgcc gctggtcggc atcactgcgc agcccgattc gagcctggcg aacgccgcca
     4441 acgtgacctt gctgctgccg ccgagcgcag aggcgtgccc gatgggactc gcccccacca
     4501 cctccaccac catgcagatg gcgctgggcg acgcgctggc ggtcgcgctg ctcacccggc
     4561 gcggcttcac ggctgccgag ttccgccagt tccacccggg cggccggctg ggcgtccggc
     4621 tgcgccgggt gcgggacgtg atgcatagcg gcgacgctat cccgctggcg ccgccggacc
     4681 tggcaatgga ccgcgcgctg ctgcgcatca ccgaaaagca tttcggctgc ctgggggtca
     4741 tcgattccga cggacggctg acgggcatcc tcaccgatgg cgacctccgc cgggcgatgg
     4801 ggcccgatct gctgtcccgg cgggtcggcg agatcatgac ccgcaaccca cggaccatcg
     4861 gccccgatgc tctggcggcc gaggccctgg gcctcatgaa cgcgcgagaa cgccccataa
     4921 ccgccctgtt tgtggtcgat tcggatggca tgccgatcgg tatcctgcat gtccacgacc
     4981 tgttgcgggc gggtgtcgca tgagcgcccg gccaggcctg gtccgctccg cccgccctcg
     5041 caccaacgtt ccactggccg cggcggcccg ctcgcggcgg ctgccgagtg ccggggctct
     5101 ggcgcggcgc cgatggctga tccggcttac caagtggctg ctgccggttt tcgccctggt
     5161 gctgctgtcg ctgctggcgc tgtggcccga aattgaccat cccaccgacg aagcgcggct
     5221 gtcgttccgc cagatgggcg gcaacatcgg cggcgcgcgg ctgaccgatg cgcgctatca
     5281 cgcggtggac aagcaaggcc gcccctacac ggtgaccgct gcggtt
//
LOCUS       DASSZH010000473         9667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_880595, whole genome shotgun sequence.
ACCESSION   DASSZH010000473 DASSZH010000000
VERSION     DASSZH010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 9667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9667
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880595"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VFN42_10265"
     CDS             <1..823
                     /locus_tag="VFN42_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8997037.1"
                     /translation="YLGEEIAQGTADEVMRNETVRRVYLGGKIETHARAKFEASGRQP
                     LLDVKDLRVLYGKAQALDGVSLHVHEGEFVSVVGLNGAGKTTLFNAISGLVPYSGEVR
                     YGGQALRGRTAASIARGGVVQCPESRELFGDMSVRENLDIGGQLLQPDERKKQLDWLM
                     DLFPRLRERESQQARTLSGGEQQMLAIARALMMKPKLLILDEPTLGLAPVILEQISKA
                     LTLLRETTPITVLLGEQNVTFALPHADRVYVLEHARIVWEGDPARFEREMGEAYL"
     gene            complement(920..1609)
                     /locus_tag="VFN42_10270"
     CDS             complement(920..1609)
                     /locus_tag="VFN42_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014105745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET8997038.1"
                     /translation="MEPCAHCDARSHSVCNAIGDRYLARLASLTQVIEAPPGTTFISE
                     GEPANHFFNITRGTAKLFKLLPDGRRQITGFATVGHFLGLAVSDTYAFSAEALEPTRY
                     CRFSRSKLRQLLDDFPAMEARLLQAAANELVAAQEQMLLLGRKTARERLASFLVTQNR
                     QSTPCGQPRARFPLPMTRGDIADYLGLTIETVSRTLTVLRKEGLIDIPGPGVLIVCDR
                     PALERVAGGLT"
     gene            1826..2326
                     /locus_tag="VFN42_10275"
     CDS             1826..2326
                     /locus_tag="VFN42_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997039.1"
                     /translation="MSPFIVRALPSDQLPAVYPLVREFAPSLEAASWLRFARQLVNGK
                     RPGQGGIVGAWRLGRSFPCGLFCYRVDNDLMRGKILVAEHFVAVDLLDPQAVLSALVE
                     ELDSLAARLGCRAVRSVVHGGNDHVHSGLAAAGHEPEASLLLKPVLAHTPPARHATRR
                     AALAHH"
     gene            complement(2355..4370)
                     /locus_tag="VFN42_10280"
     CDS             complement(2355..4370)
                     /locus_tag="VFN42_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET8997040.1"
                     /translation="MQTVRVACGHDCPDMCSLLAHVEDGRILRVEGDPDQPFTAGFAC
                     AKVNRDAELVHSPRRLTTPLRRTGPKGAGQFTPITWDAALEAITANWRSIIAESGPLA
                     LLGYAYSSHQGQMNRGLLNGLFHALGSSRLWAGTVCDSCADAAWEATVGPIGGADPES
                     VAQSDLVVCWGADLFATNVHFWAKLEQVRQRGVKLVVIDPRRTRAAQAADWHIPIRIG
                     TDAALALGIMHILVRDGLADRNYIAQHTLGFERVEREILPRFPPARVAAITGVPQADV
                     ERLAAMYGAAKRSFIRLGWGMTRLRHGGQALRSVALLPGVTGAYGRYGGGALSGTNAS
                     FELNYNALRKPSGPASARTVNHLRLGEALLTLRDPPIRALFIASNNPAVTCPNAMQTR
                     AGLAREDLFTVVHDPFLTLTARYADIVLPAATYLETEDFFRAYGTYYMQYSPRAVAPQ
                     GQAWSNMRLAQELAQRMGLQDAVFRMSERELLRELFRGASGAAAAFDPAALADAGPVS
                     IAVARGQEFRTPSGKLEFYSEDLARQGVPPLPDWQPDPEEPQDNRFPLRLLTAPGYFQ
                     SHTTFAGVSFLREREGRPICVLHPQDAAARTLRDGQVVRLFNERGAVGLTLRVRDEVQ
                     PGVVLVPGQRSDEETESGTVNMLCGDSFTDMGDGATYQSTWLDAAAL"
     gene            complement(4459..5016)
                     /locus_tag="VFN42_10285"
     CDS             complement(4459..5016)
                     /locus_tag="VFN42_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET8997041.1"
                     /translation="MVLQWPAADKLYAEFREQAAMTVFFTADTHFHHAGAIGFYRRPF
                     ASAADMDAAMLARWNEAVGPGDEVWHLGDFALRLAAEPAGALLRQLHGRKHLVTGNND
                     PPEIAALPGWASVQAYAEILLDGTRLVLCHYPFRTWNGLARGALNLHGHSHGRLKPVP
                     RQIDVGVDVWGFRPVTLAQLRAGPG"
     gene            complement(5111..5782)
                     /locus_tag="VFN42_10290"
     CDS             complement(5111..5782)
                     /locus_tag="VFN42_10290"
                     /inference="COORDINATES: protein motif:HMM:NF013911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative oxidase"
                     /protein_id="HET8997042.1"
                     /translation="MPQQIDLSIHHRPQGASDRFAFGFVKSLRFFADTFFARRYGHRA
                     IVLETVAAVPGMVGATLNHLKCLRRMKDDRGWIRTLLDEAENERMHLMTFIAIARPNI
                     LERLLVLLVQGGFYNAFFLLYLISPKTAHRVVGYFEEEAIISYNHYLAEIDSGRIANV
                     PAPAVARRYWNLRADATLRDVVIVVRADEANHRDVNHGFADELAGGPAAAVACYLQQP
                     PGRMA"
     gene            6020..7042
                     /locus_tag="VFN42_10295"
     CDS             6020..7042
                     /locus_tag="VFN42_10295"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HET8997043.1"
                     /translation="MARSADEHPVRFDLLTENPVYKPFRYPWAYEAWLTQQRVHWLPE
                     EVPLADDVKDWHRNLTDGERNLLSQIFRFFTQADVEVNNCYMKHYARVFKPTEVLMML
                     SAFSNIETVHIAAYSHLLDTIGMPEIEYQAFLRYQEMKDKYDYMQGFSVGSKHEIART
                     LAAFGAFTEGLQLFASFAILLNFPRWGKMKGMGQIISWSVRDETLHCNSIIRLFRTFV
                     QENPEIWTEELRRDIYLSCATIVDHEDAFIDLAFEQGAVQGLTAAEVKQYIRYVADRR
                     LVQLGLNPLYHVARIPLPWLDDMLNAVEHTNFFENRATEYSKASTTGSWEEAFAVPPD
                     TAAAPL"
     gene            7357..7680
                     /locus_tag="VFN42_10300"
     CDS             7357..7680
                     /locus_tag="VFN42_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997044.1"
                     /translation="MEQMATALDSADTADTAATQPEGEPGRESSSSGGAPRYSRRLSD
                     KILIAFHHACDQGDFEVAEQLLHVLEMMLTRRPLTPDGTRRRNMESLVAAHERLWHLR
                     HPDAD"
     gene            complement(7717..8481)
                     /locus_tag="VFN42_10305"
     CDS             complement(7717..8481)
                     /locus_tag="VFN42_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012552941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET8997045.1"
                     /translation="MPAKSPDDGAAALPPAVTGGHLLGGRVRYLQPAEGFRSGIEPVL
                     LAAAVPARPGERILEGGSGAGAALLCLAARVAGVIGTGVERDPALAELAAANAAANGL
                     PLQFIAAELETAALQGPFDHAMANPPYHAAGGPASPDAARETAKRGAPGLLTAWAERL
                     GGSLRRHGSLTLILPAWRVAECLLAMQAAQCPPAAIQPLWPKAGREAKLVLVQGRRFG
                     RAPLRLLPGLALHEADGSFTPRAEAVLRGGGKLDWG"
     gene            complement(8462..8671)
                     /locus_tag="VFN42_10310"
     CDS             complement(8462..8671)
                     /locus_tag="VFN42_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HET8997046.1"
                     /translation="MRVVVTSNDPVRLSFLAALLADAGIEAVLLDTHTSIVEGSIGAI
                     QRRLVVASDDYSKARRILTDAGEIA"
     gene            8821..>9667
                     /locus_tag="VFN42_10315"
     CDS             8821..>9667
                     /locus_tag="VFN42_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014104671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET8997047.1"
                     /translation="MSAVSARPDEDALSALVALVGGDIEACNRAIIARMDSPVSLIPQ
                     LAAHIVAAGGKRLRPLLTLAAARLCGYAGDRHVHLAACVEFIHTATLLHDDVVDESQL
                     RRGLASANAVFGNKASVLVGDFLFARAFQLMVDDGSLKVLGILSQAAATIAEGEVLQL
                     VTQNDLSTPEERYLEVIKGKTAALFAAACQVGAVVADRPEIEERALAEYGLNLGIAFQ
                     LVDDALDYAADQATLGKTVGDDFREGKITLPVLVAYQAGDADERAFWRRTIETSEQME
                     ADLEQA"
BASE COUNT         1589 a         3183 c         3351 g         1544 t
ORIGIN      
        1 gtacctgggc gaagagatcg ctcaaggcac cgccgacgag gtgatgcgca acgaaacggt
       61 gcgccgcgtg tacctgggcg gcaagatcga gacgcatgca cgtgcgaaat tcgaagcgag
      121 cggccgtcaa ccgttgctgg atgtgaagga cctgcgcgtg ttgtacggca aggcgcaggc
      181 gctggatggc gtatcgctgc acgtgcatga aggggaattc gtctcggtgg tcggtctgaa
      241 cggcgccggc aagaccacgc tgttcaatgc gatctccggc ctggtgccgt attccggcga
      301 ggtccgctat ggcggccagg cgctgcgcgg ccgcaccgcc gcctcgattg cccgcggtgg
      361 ggtggtgcag tgtccggaaa gccgcgaact gttcggcgac atgagtgtcc gggagaacct
      421 ggacatcggc ggccaactgc tgcagcccga tgagcggaag aagcagcttg attggctgat
      481 ggatctgttt ccccggctgc gcgaacgcga atcgcagcag gcgcgtacct tgtccggcgg
      541 cgagcagcag atgctggcga tcgcccgcgc gctgatgatg aagccgaaac tgctgatcct
      601 ggatgagccg acgctgggcc tggcgccggt gatcctggag cagatctcca aggcgctgac
      661 tttgctgcgc gagaccacgc cgatcaccgt gttgctgggc gagcagaacg tcacgttcgc
      721 cctgccgcac gcggaccggg tgtatgtgct ggagcatgcg cggattgtct gggaaggcga
      781 tccggcacgg ttcgagcgcg aaatgggcga ggcgtacctg taggactgcg gaccgtcacc
      841 tcgatgtgcg catgtcgtgg cgggcgaagg cccgtcacgc aatcggatga ttccggtcgg
      901 tgccttgacc agcgcctggc taggtcaatc cgccggcgac acgttccagc gccggccgat
      961 cgcacacgat cagcacgccc ggaccgggga tgtcgatcag gccttccttg cgcagcacgg
     1021 tcagggtgcg gctgacggtc tcgatggtca gccccaggta atcggcgata tccccgcgcg
     1081 tcatcggcag cggaaagcgc gcccgtggct gcccgcaggg ggtggactgg cggttctgcg
     1141 tcaccaggaa ggaggcgagc cgctcgcgcg ccgtcttgcg gccgagcagc agcatctgct
     1201 cctgcgccgc caccagctcg ttggccgcag cctggagcag gcgcgcttcc atcgccggga
     1261 agtcgtccag caactggcgc agcttgctgc gggaaaaccg gcagtagcgg gtcggttcca
     1321 gcgcctcggc actgaaggcg taagtgtcgg acaccgcgag gccgaggaag tgccccaccg
     1381 tcgcgaatcc ggtgatctgc cggcgcccgt ccggcagcag cttgaacagc ttggcggtgc
     1441 cgcgggtgat gttgaagaaa tggttggccg gttcgccttc ggagatgaag gtggtgcccg
     1501 ggggggcttc gatgacctgg gtcagtgacg ccaggcgggc gagatagcgg tcgccgatcg
     1561 cattgcacac gctgtgcgag cgtgcatcgc aatgggcaca gggttccacg ggagcttggg
     1621 ggtcaagcac aaccgggcgg gacagcacgg cactcatgga gggacctcga gcgagcacaa
     1681 ggctcacatc acgatgtcgg ccagcttcgg ggggcgtgca atacgggata tcccgtacct
     1741 ccgtcggagc gaggcttgat ctgcgtcaac gcgagaggct gtcggcttgc ggcatgcttc
     1801 tgggaccaga ggggaattcg ccgctatgtc gcccttcatt gtccgtgcac tgccatccga
     1861 tcaactgccg gcggtctatc cgctggtccg tgagttcgca ccgtcgctgg aggccgcgtc
     1921 ctggctccgc tttgcccggc aactggtaaa cggcaagcgg ccaggacagg ggggaattgt
     1981 cggcgcctgg cgcctggggc gcagctttcc ctgcggcctg ttctgctacc gggtggacaa
     2041 cgacctgatg cggggcaaga tcctggtggc ggagcatttc gtcgcggtgg acctgctcga
     2101 cccgcaggcg gtgctgagcg ccctggtgga agaactcgac tcgctggccg ctcggcttgg
     2161 ctgcagggct gtgcggagcg tggtgcatgg cgggaacgac cacgtccact ccggtctggc
     2221 tgccgcggga catgagccgg aagcatcgct gctgctgaag cccgtgctgg cccacacgcc
     2281 gccggcgcgg cacgcgacgc ggcgggccgc cctggcgcat cactgaccag gccggtttgt
     2341 cccggctgag cggcctacag ggccgcggcg tccagccagg tgctctgata ggtcgcgccg
     2401 tcgcccatgt cggtgaagct gtcgccgcac agcatgttca ccgtgcctga ttcggtctcc
     2461 tcgtccgaac gctgacccgg aaccagcacc accccgggct gcacctcgtc ccgcacccgc
     2521 agcgtcaatc cgaccgcacc gcgctcgttg aacagccgca caacctggcc gtcccgcagc
     2581 gtgcgcgccg cggcatcctg cgggtgcagc acgcagatcg gcctgccttc ccgttcgcgc
     2641 aggaagctga cgccggcgaa ggtggtatgg ctctggaaat agccgggagc ggtcagcagg
     2701 cgcagaggaa accggttgtc ctgcggttcc tccgggtccg gctgccagtc cggcagcggc
     2761 ggcacgccct ggcgcgccag gtcttcggaa tagaactcca gcttgccgga cggcgtgcgg
     2821 aattcctgtc cgcgcgcaac agcgatcgag accggtccgg catcggccag cgcggccgga
     2881 tcgaacgccg ccgccgcgcc gctggcgccg cggaacagct cccgcagcaa ctcccgttcg
     2941 gacatgcgga acaccgcatc ctgcaggccc atgcgctggg cgagttcctg cgccaggcgc
     3001 atgttcgacc aagcctgccc ctgcggcgcc acggcgcgcg gactgtattg catgtagtag
     3061 gtgccgtagg cgcggaagaa gtcttccgtt tccaggtagg tcgccgccgg caggacgata
     3121 tcggcgtagc gcgcggtgag cgtcaggaac ggatcgtgca ccacggtgaa caggtcctcg
     3181 cgcgccaggc cggcacgggt ctgcatcgcg ttggggcaag tgaccgccgg gttgttgctg
     3241 gcgatgaaca gcgcgcggat cggcgggtcg cgcaaggtca gcagcgcctc gcccaggcgc
     3301 agatggttca ccgtgcgggc gctggcggga ccggacggct tgcgcagcgc gttgtagttc
     3361 agctcgaagg atgcattggt gccgctcagc gcgccaccgc cgtagcggcc ataggcgccg
     3421 gtgacgcccg gcagcagcgc aacgctgcgc agcgcctggc cgccgtggcg caggcgtgtc
     3481 atgccccagc ccagccggat gaaggatcgc tttgccgcgc cgtacatcgc cgccagccgc
     3541 tcgacatcgg cttgcggcac gccggtgatc gccgccacgc gcgccggcgg gaaacgcggc
     3601 aggatctcgc gttcgacacg ctcgaacccg agcgtgtgct gggcgatgta gttacggtcg
     3661 gccaggccgt cgcgcaccag gatgtgcatg atacccaagg cgagcgcggc gtcggtgccg
     3721 atgcggatgg ggatgtgcca gtcggccgcc tgtgccgcgc gggtgcggcg cgggtcgatc
     3781 accaccagct tcacgccgcg ttggcgcacc tgctccagct tcgcccagaa atgcacgttg
     3841 gtggcgaaca ggtcggcgcc ccagcagacc accaggtcgg actgcgccac cgattccggg
     3901 tcggcgccgc cgatcgggcc gaccgtggcc tcccaggccg catcggcgca gctgtcgcag
     3961 accgtgccgg cccacagccg gctgctgccc agggcatgga acaagccgtt cagcagcccg
     4021 cggttcatct gcccctggtg ggaactgtag gcatagccaa gcagcgcgag cggtccggac
     4081 tcggcgatga tgctgcgcca gtttgcggtg atcgcctcca gcgccgcatc ccaggtgatg
     4141 ggggtgaact gtcccgcacc tttcggtccg gtgcggcgca gcggcgtggt cagccggcgc
     4201 ggcgaatgca ccagttcggc gtcgcggttc accttggcgc aggcgaatcc ggcggtgaag
     4261 ggctggtcgg ggtcgccctc gacacgcagg atgcgtccgt cctccacatg cgccagcagc
     4321 gagcacatat cgggacaatc gtgcccgcag gccacgcgaa cagtttgcac ttcggcctcc
     4381 attgccacag caccggcatg cgtgccgaag agccagcctg gcatatagag ccggttgggc
     4441 cggacagcgc cggaccaatc agccagggcc ggcgcggagt tgcgccaggg tgaccgggcg
     4501 aaacccccag acatcgacgc cgacatcgat ctggcggggc accggtttca gcctgccgtg
     4561 gctgtggccg tgcaggttca gcgcgcccct ggcgaggccg ttccaagtgc ggaacgggta
     4621 gtggcacagc accagccgcg tgccgtccag caggatttcc gcataggcct gcacgctggc
     4681 ccagcccggc agcgccgcga tctccggcgg atcgttgttg ccggtcacca gatgcttgcg
     4741 gccgtgcagt tgccgcagca aggcccccgc gggttcggcc gccagccgca gcgcgaagtc
     4801 gccgaggtgc cagacctcgt cgcccggacc caccgcttca ttccagcgcg ccagcatcgc
     4861 cgcgtccatg tccgcggccg acgcgaaagg ccggcggtag aagccgatgg cgccggcgtg
     4921 gtggaagtgg gtatcggcgg tgaagaacac cgtcatggcg gcctgctcgc ggaattcggc
     4981 gtagagcttg tccgcggccg gccattgcaa caccatggcc gcgccgctcg accgcccgcc
     5041 ggactgggtc cggagagcgg ccgagtaccc tatcaagccg cgcgcttgcc gcccatggga
     5101 ggcgccggga tcaggccatc cggccgggag gctgctgcag atagcaggca acggcggccg
     5161 ctggtccacc ggccagctca tcggcaaatc cgtggttcac gtcgcgatgg ttcgcctcgt
     5221 ccgcgcgcac cacgatgacg acgtcccgca gcgtggcgtc ggcgcgcagg ttccaatagc
     5281 gcctggccac ggccggcgcc ggcacattgg caatgcggcc gctgtcgatc tccgccaggt
     5341 agtggttgta ggagatgatg gcttcttcct cgaaatagcc gaccacacgg tgcgcggtct
     5401 tcggcgagat gaggtacagc aggaagaagg cattgtagaa cccgccctgc accagcagga
     5461 ccagcagccg ctccaggatg ttcggcctgg cgatcgcgat gaaggtcatc aggtgcatgc
     5521 gctcgttctc ggcttcgtcg agcagggtgc ggatccagcc gcggtcgtcc ttcatgcggc
     5581 gcaggcattt caggtggttc agcgtggcgc cgaccatgcc cgggaccgct gccaccgtct
     5641 ccagcacgat ggcgcggtgg ccgtagcgcc gggcgaagaa cgtgtcggcg aagaagcgga
     5701 gggatttgac gaagccgaag gcgaagcggt cggaggcacc ctgcggccgg tggtggatgg
     5761 agaggtcgat ctgctgcggc atggaagctg ttcctttcgt cccctggggc gcccgcaagg
     5821 cggcaccagg catgccgggg tggtagctcc gcttcggcgg gcaccaattg atccagatca
     5881 cggccgaggc gaacgaatgt cactcgtcgg cggcgcctcg cgcgcttcac gccgccggaa
     5941 gtcggtttcc cgactcgccg ccaggcggat atcctgtccc accacccctt gaatcgcgag
     6001 gatctgccgc atggacggaa tggcccgcag cgccgatgag cacccggtca ggttcgacct
     6061 gctgaccgag aacccggtgt acaagccctt ccgctatccc tgggcgtacg aggcgtggct
     6121 gacccagcag cgcgtgcact ggttgccgga ggaggtgccg ctggccgacg acgtcaagga
     6181 ctggcaccgc aacctgaccg atggcgagcg caacctgctg tcgcagatct tccgcttctt
     6241 cacccaggcg gacgtggagg tgaacaactg ctacatgaag cactacgcgc gggtgttcaa
     6301 acccaccgaa gtgctgatga tgctgtcggc cttctccaat atcgagacgg tgcacatcgc
     6361 cgcctacagc catctgctcg acacgatcgg catgccggag atcgagtacc aggccttcct
     6421 ccgctaccag gagatgaagg acaagtacga ctacatgcag gggttctcgg tcggttcgaa
     6481 gcacgagatc gcccgcacgc tggcggcgtt cggcgccttc actgaggggc tgcagctatt
     6541 cgcctccttc gccatcctgc tgaactttcc ccgctgggga aagatgaagg ggatggggca
     6601 gatcatctcc tggtcggtgc gggacgagac gctgcactgc aactcgatca tccggctgtt
     6661 ccgcacattc gtgcaagaaa atccagaaat ctggacagaa gagctgcggc gcgacattta
     6721 cctaagttgc gccaccatcg tggatcacga ggacgcgttc attgacctgg cattcgaaca
     6781 gggcgccgtg caggggctga ccgcggccga ggtgaagcag tacatccgct acgtcgccga
     6841 ccggcggctg gtgcaacttg gcctcaaccc gctgtatcac gtagcgcgga ttccgttgcc
     6901 ctggctggac gacatgctga acgcggtgga acacacgaat ttcttcgaga atcgcgccac
     6961 cgaatacagc aaggcctcga cgacggggag ctgggaggag gcctttgccg tgccgcccga
     7021 tacggccgcg gccccgctct gagccgcgta cggtcgcatt cgtccaaatt tcgacgaatt
     7081 ttgctccgat tctgacggtt cctctggcgg cccccagggg caggctgtat agtccctcct
     7141 tgcgccggtc ggcttcccac tgtcatgcgg ccgaacgtgc gcaagtaacg gtcgtattgc
     7201 gccggcataa cgggttgcgg ctggcggctg gaggatattg gatcaggatg cgtcaccatc
     7261 ggcgtgatac cgtccctgcg ggggtacaac caatgggagc atcggcgcgc agccatgcca
     7321 aggggcggta aaggcagggg tcgcaggaaa cgggacttgg aacagatggc cacagcactt
     7381 gattccgcgg acaccgccga tacggctgca acgcagcctg agggagagcc cggtcgcgaa
     7441 tcctcttcgt ccggtggcgc tccgcgctac agtcgccggc tgtccgacaa gatcctgatc
     7501 gccttccacc atgcctgcga tcagggggat ttcgaggtgg cggagcagtt gctgcacgtc
     7561 ctggaaatga tgctgacgcg ccggccgctg actcccgacg gtacccgacg gcgcaacatg
     7621 gaaagcctcg tggccgcgca tgagcggctc tggcacctgc gtcaccccga tgccgactga
     7681 gcacgcttcg cccggcagcg tggtccggct ccgcggctag ccccagtcca gcttgccgcc
     7741 accacgcagc accgcttcgg cgcgcggagt gaaactgcca tcggcctcgt gcagcgccag
     7801 ccctggcagc agccgcagcg gcgcgcgccc gaaccggcgt ccctgaacca gaaccagctt
     7861 ggcctcccgg ccggctttcg gccagagtgg ctgaatcgcc gcgggcggac actgcgcggc
     7921 ctgcatcgcc agcaggcact cggcaacccg ccaggccggc aggatcaggg tcaggctgcc
     7981 atgccgccgc aggctgccgc cgagccgctc ggcccaggcc gtcaacaatc cgggcgcgcc
     8041 ccgcttcgcg gtctcccgcg ccgcatcggg cgaggcgggg ccgcccgccg catgataggg
     8101 cggattggcc atggcatggt cgaacgggcc ttgcagcgcg gccgtctcca gctcggcggc
     8161 gatgaattgc agcggcaacc cgttggctgc ggcgttggcc gcggccagtt cggccaaagc
     8221 cgggtcgcgt tccacgccgg tcccgatcac cccggcaacc cgcgccgcca ggcagagcag
     8281 cgcggcaccc gcgccgctgc caccttccag gatccgctcg cccggcctgg ccggcaccgc
     8341 agcggcgagc agcacaggct cgatgccgct gcggaacccc tcagcgggtt gcaggtagcg
     8401 gacgcgcccg cccagcaggt ggccgccggt gacagccggg ggaagcgcgg cggcgccgtc
     8461 gtcaggcgat ttcgccggca tcggtcagga tccggcgtgc cttgctgtaa tcgtcgctgg
     8521 ccaccaccag ccgccgctgg atggccccga tgctgccttc cacgatactg gtgtgcgtgt
     8581 cgagcaacac cgcctcgatc ccggcatccg ccagcagcgc ggccagaaaa gacagccgga
     8641 ccggatcatt ggatgtcacg acaacacgca tatctctatg gtgcgggcag gttggatact
     8701 tggcaatcgg cgattgcctt gaccgtcgga ccgcgaggcc ggtaatgacc agccaatcaa
     8761 tgatgcagcc aggagatgag tgtgggcgtc gccactcctc tggagtcaga cgtggcgccg
     8821 atgtcggctg tgtcggcacg gccggatgaa gatgcgctga gcgcgctcgt cgcgctggtc
     8881 ggcggggata tcgaggcgtg caaccgcgcc atcattgcgc ggatggacag cccggtatcg
     8941 ctgatcccgc aactggcggc gcatatcgtc gcggccggcg gcaagcgcct gcgtccgctg
     9001 ttgaccctcg ctgccgctcg cctgtgcggc tatgccgggg accggcatgt ccatctcgcc
     9061 gcctgcgtcg aattcatcca caccgcgacc ctgctgcatg acgatgtggt ggacgaaagc
     9121 cagttgcgcc gcggcctggc ctcggccaac gcggtattcg gcaacaaggc ctcggtgctg
     9181 gtcggcgatt tcctgttcgc ccgcgcgttt caactcatgg tggatgacgg ctcgctgaag
     9241 gtgctgggca tcctgtcgca ggccgcggcc accatcgccg agggtgaggt gctgcaactg
     9301 gttacccaga acgatctgtc cacgccggag gagcgctacc tcgaggtgat caagggcaag
     9361 accgcggcgc tgttcgcggc agcctgccag gtgggtgcgg tggtggccga ccggccggag
     9421 atcgaggagc gtgccttggc cgaatacggc ctgaacctgg gtatcgcctt ccagctggtc
     9481 gatgacgcgc tggattatgc ggccgaccag gcgacgctgg gcaagaccgt cggcgacgac
     9541 ttccgcgagg gcaagatcac cctgccggtg ctggtcgcct atcaggccgg cgacgccgac
     9601 gagcgcgcct tctggcgacg gacgatcgag accagcgagc agatggaggc cgatctggag
     9661 caagcgc
//
LOCUS       DASSZH010000474         8681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1494232, whole genome shotgun sequence.
ACCESSION   DASSZH010000474 DASSZH010000000
VERSION     DASSZH010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8681
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494232"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VFN42_10320"
     CDS             complement(<1..589)
                     /locus_tag="VFN42_10320"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8997048.1"
                     /translation="MATPSSGSRSFPSDTEILGYSRYVVLAAAFLSMAVISPYEYAWS
                     SMAGHIGGQYHWSHIQIGWMFTLFVIFESIGTLPGGILRDKFGPRWVTAIAGLIGGVG
                     IYATSLGPNYGFVLVLWCIGSFFAGFVYNTAVTTANKWFPDKRGFTAGLIAAAFGWGS
                     LPFIFPIRAIAKTAPISVFFNVIYIMAAIIGGVCIV"
     gene            799..1626
                     /locus_tag="VFN42_10325"
     CDS             799..1626
                     /locus_tag="VFN42_10325"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET8997049.1"
                     /translation="MQVQLAAARQSLQGMASGFIHRLPYIGLALLAYLLFHFTALGLR
                     ALIMRVAERRQAHIAGALVLGRLVQATVIALGVLVALVIIIPSFQIGQIVQLLGISSV
                     AVGFAFRDVLQNFLAGILILFNQPFRIGDQIVVGGFEGMVETIETRATFIRTYDGRRV
                     VIPNATLFNQSVTVNTAYAARRLQYDLHLDDAGDIESARADILQALRAVPSVLPDPAP
                     DVVTTDVAGKGVTFRIRWWVQPPRRSDVTASQDEALAAIRRELDRRQNEMAPPAAAS"
     gene            complement(1675..1944)
                     /locus_tag="VFN42_10330"
     CDS             complement(1675..1944)
                     /locus_tag="VFN42_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997050.1"
                     /translation="MRCLPLALTRGSLLSQVMREAQQDPRRQGLCTCCGARIPIRRDR
                     PGAEVRCPACLRLQPADVVAERPWRLTAASAEALRLIKSWPRHYS"
     gene            complement(2083..4332)
                     /locus_tag="VFN42_10335"
     CDS             complement(2083..4332)
                     /locus_tag="VFN42_10335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8997051.1"
                     /translation="MSERATRQAPESAKPVSLVAGAPLRGLGSTVALWLNRASRLGVL
                     RRTVNRKRLRKAWELARHAGLRVFWTEVRLHISYRSRLGIPAQPEALPPSSSSPDPYE
                     EVEWHRDRPLVSVIIPCFNYGAFVRQAVDSVLAQTFQDLEVIVVEGGSTDGTTPDIVR
                     QLQAPRVTVLLRDEPHRAGENRNFGIRSARGKYVCCLDADDLLHPTYIEKAVFLLEHY
                     GYDVVSTAVRRFGAVEETYGVLPAPDLADLLEGNHVTTCAVFRRSFWELAGGYEDSRP
                     DQVYLHEDWRFWIRVACHGARFANITGEQLFLYRAHTNASLSSKPGMLPNAVQGKLIQ
                     ELEQALVTPAALKRSRESARVCMRSTAGARNMVRSTGKDARCRTILLAVPFLVLGGAE
                     RLLSEVLRHLRSLGYRIVIVSTVHWLPELGDTTSWFEPITQEIYHLPRFLSEDRWSDF
                     LDYLLVAKQADALWIVGSPFTYQALPELTRNHPHLLVADLLFNTVGHGENNRRYSRYI
                     NLNLVESQEVRQWLAEGGESEERIRIVPSGVDLDLYKPGTASPALREQLGLDADSFIV
                     GFSGRLSEEKAPLTVLRIAEQLPEDSRVRFLMTGTGPLGEPLRDAIRRRGLERRVQFV
                     GMVPEVRDYLACYDVLLLPSMFDGRPTVVLEALAMGVPVIASSVGGLPELVEHGVNGY
                     LCPAKDVAAFARHITALQRDPALHARMRAAARQFAEQNLGIERMTARYAQAFDDLFRL
                     HEPADASGN"
     gene            4721..5884
                     /locus_tag="VFN42_10340"
     CDS             4721..5884
                     /locus_tag="VFN42_10340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET8997052.1"
                     /translation="MNVLVWQWGRFGGAPRVAVALVQGMKTLPNVNVSLSMSRNAELM
                     RDGSMPPCTLPVDTYRGRLGFLWRFATIPVAARSLARRIARLRPDVAICAQTGPLDLL
                     MARALRLLGIPMVVLIHDADLHPGDGKLFQMRLQRALCRRAAALGALSAHVGARLKAQ
                     GLAGNGARPLIQLRLPPLPIAVPAAEPPAGSGFRLLIFGRLLPYKGLDMLAEALSRLG
                     PLPGLTVRVVGLGPESSELKQLRAIPNVTVENRWVPESELPQLLAWADALVLPYREAS
                     QSGVAALALGAGRHVLATRVGGLSEQLGNEPLAILCDPDPVSLSEALRTLVAASPSAS
                     TAPAADPIAAWRDMAEELLRQIAACRPVQADRVARCGAVVPGGLNLNGAAGQD"
     gene            complement(5863..5937)
                     /locus_tag="VFN42_10345"
     tRNA            complement(5863..5937)
                     /locus_tag="VFN42_10345"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5903..5905),aa:Thr,seq:ggt)
     gene            6065..6979
                     /locus_tag="VFN42_10350"
     CDS             6065..6979
                     /locus_tag="VFN42_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007281969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET8997053.1"
                     /translation="MWLHTMPSRHIARLETVSVTVPDAALEAYEAALDSTCDTVGFFR
                     DEDSGDWRVEGVKPVGAGEAELSASLALAAGLSGFVARLERSDTPAEGWLARSYAGFP
                     EQRIGQHFAIRGTHLPPETVAGRITLLLDAGLAFGSGEHGSTRGCLLALERVARRAPR
                     RILDMGTGSGILAIAAARLLRRPVLATDIEPWSVRVAGDNARLNGVAHLVRPLRADGW
                     RDARVRAGGPYDLVFANILARPLCLMAHDLAAQLAPGGTAILAGLLSTQARAVLAAHR
                     RQGLRLERMIVVAPWATLVLRRTAVERA"
     gene            complement(7021..7332)
                     /locus_tag="VFN42_10355"
     CDS             complement(7021..7332)
                     /locus_tag="VFN42_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HET8997054.1"
                     /translation="MSAPIAKDQLAYQLPNLTYVDAHPEEPNLGRVTLPTELPKPSFG
                     RWLAARIAALQAWRERQSAMAELEMMSDRELMDIGLSRADMARVFDSAHNADLVARSE
                     G"
     gene            7533..>8681
                     /locus_tag="VFN42_10360"
     CDS             7533..>8681
                     /locus_tag="VFN42_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HET8997055.1"
                     /translation="MNGTRLASLRAELARQGLDGFIVPRADEHLGEYVPAAAERLAWL
                     TGFTGSAGLAAVLPDRAAVFTDGRYVLQLAEQTDPSLWQRKHITEEPPPAWLAAQAQP
                     AARIGYDPLLISEEALGRYTEAGLKMQPVAHNPVDAVWTGRPAPPAAPAMPHALAYAG
                     RSAEDKRQEIAAALQAAKQDAAVLSDPSSIAWLLNIRGGDVPFTPFALGFALVHADGG
                     TELFMDPAKLSDETRAWLGNAVAVAGREALGPALSRLGGKRVRVDPGGSPVWFSQMLR
                     DAGATVVAGPDPCLLPKACKNPVEQQGARNAHARDAVAMVRFLHWLSQVGPGGGETEL
                     SAADQLLAFRREVDGFRGESFPAISGAGEHGAVIHYRVTPESNRPIRPN"
BASE COUNT         1458 a         2890 c         2913 g         1420 t
ORIGIN      
        1 ccacgatgca cacgccaccg atgatggctg ccatgatgta gatgacattg aagaagaccg
       61 aaatcggcgc ggtcttcgcg atggcgcgga tcgggaagat gaagggcagc gacccccaac
      121 cgaacgcggc ggcgatcagc ccggcggtga agccgcgctt gtccgggaac cacttgttcg
      181 ccgtcgtcac tgccgtgttg tagacgaacc cggcgaagaa ggaaccgatg caccacagca
      241 cgagaacgaa cccgtagttc gggccgagcg aggtggcata gatgccgacg ccgccgatca
      301 ggccggcaat cgcggtgacc cagcgggggc cgaacttgtc gcgcaggatg ccgcccggca
      361 gcgttccgat cgattcgaag atcacgaaca gcgtgaacat ccagccgatc tggatatgcg
      421 accaatgata ctgcccgccg atatggccgg ccattgaact ccaggcgtat tcgtaggggc
      481 tgatcaccgc catcgacagg aaagcggcgg ccagtaccac ataccggcta tagccgagta
      541 tttcagtatc ggagggaaaa ctgcggcttc ccgaggaagg tgttgccatg gacatggcct
      601 cccttttttt gatgaacgtt tatccagaca cgatgtatag tgtatgaggc ataccaacca
      661 tgattgatct ggtcaatgca atccatgggt aaaattgatg gatgattttg ccggttggca
      721 aaataaccaa agccgcgctg aacagcgtgt ccggctgacc cagcggcaac cgaagcgggc
      781 cgcggacgtt gcccaggaat gcaggtccag ctcgccgccg cccggcagtc gctgcagggg
      841 atggcctccg gcttcatcca ccgcctgccc tatatcgggc tggctctgct ggcttatctg
      901 ctgttccact tcaccgcgct gggcctgcgc gcactgatca tgcgcgtcgc cgagcggcgc
      961 caggcgcaca tcgccggagc gttggtgctg ggacggctgg ttcaggccac cgtgatcgca
     1021 ctgggggtgc tggttgcgct ggtcatcatc atcccgtcct tccagatcgg ccagatcgtt
     1081 cagttgctgg gcatcagcag cgtggcggtc ggtttcgcat tccgcgatgt gctgcagaat
     1141 ttcctcgccg gcattctgat cctgttcaat cagcccttcc gcatcggcga ccagatcgtg
     1201 gtgggcgggt tcgaaggcat ggtcgaaacc attgagacac gcgcaacctt catccgcacc
     1261 tatgatggac ggcgggtggt gattcccaac gcgacattgt tcaatcaatc cgtcacggtg
     1321 aacaccgcct atgcggcgcg ccggctgcag tacgatctgc atctggacga cgccggcgat
     1381 atcgagagcg ccagggccga catccttcaa gcgctgcgcg ccgtgccgag tgtgttgccg
     1441 gatccggcgc cggacgtggt gacaaccgat gtggccggca aaggcgtgac gttccgcatc
     1501 cgctggtggg tgcagccccc gcgccgttcg gatgtcaccg catcgcagga cgaggcgctg
     1561 gcagcgatcc gtcgcgaact ggaccgccga cagaatgaga tggcaccccc ggcggccgcc
     1621 tcgtaaccgt actcaggtat cgggggatgc ggccgaggct ttcgcggacc gcggtcagga
     1681 gtagtgccgc ggccaggact tgatcaggcg cagcgcctcg gccgaggcgg cggtgagccg
     1741 ccagggacgc tccgcgacca catcagccgg ctgcaggcgc aggcaggccg ggcagcgcac
     1801 ctcggcgccc gggcgatcgc gtcggatggg gatacgggcg ccgcaacagg tgcacaggcc
     1861 ttgccttcgc ggatcctgct gcgcctcgcg catcacctgc gacaacagcg accctctggt
     1921 cagtgccagc ggcagacacc tcatggtcga cctccccagc ccgttccggt ttgatagcgc
     1981 cgtcgcaacc ggttttgcaa tgcgcgcggc agccgggaaa caattcggca gcgcaccatc
     2041 acccggcggg cagcgtccgc gcaccggtcg ccagcccgcc ggctagttcc ctgaagcgtc
     2101 ggccggttca tgcaagcgga acaggtcgtc gaaggcctgg gcgtaccgcg ccgtcatccg
     2161 ctcgatgccc aggttctgct cggcaaactg ccgagcggcg gcacgcatcc gggcatgcag
     2221 tgccggatcg cgctgcaacg ccgtgatatg gcgggcaaag gcggccacgt ccttggccgg
     2281 gcagagatac ccgttgacgc catgctcgac cagttccggc aagccaccga cggacgaggc
     2341 aatcacgggc acgcccatcg ccagcgcttc gagaaccact gtcggacgcc catcgaacat
     2401 ggacggcagc agaagaacgt cgtagcacgc aaggtagtcc cgcacttcgg gaaccatgcc
     2461 gacgaactgc acgcggcgtt ccagtccccg cctgcggatg gcatcgcgca gtggttcccc
     2521 cagcgggccc gtccccgtca tcagaaagcg cacgcgcgaa tcttccggga gctgctcggc
     2581 gattcgcaag acagtgagcg gcgctttctc ctccgacagt cggccggaaa agccaacgat
     2641 gaaggaatcc gcatccagcc cgagctgttc gcgcaacgcg ggcgacgcgg tgcccggctt
     2701 gtacaggtcc aggtcaaccc cgctggggac gatccggatg cgctcctcgc tttctccacc
     2761 ttcggccagc cactggcgga cctcctgaga ctcgacgagg ttcaggttga tgtagcggct
     2821 gtagcgccgg ttgttctcgc catgcccgac cgtgttgaac agcagatcgg ccaccagcag
     2881 atgcgggtgg tttcgggtca gttccgggag cgcctggtag gtgaacgggc tgccgacgat
     2941 ccacagcgca tccgcctgtt tcgcgaccag cagatagtcg aggaagtcgc tccagcggtc
     3001 ctcggacagg aaccgcggca gatgatagat ctcctgcgtg atgggctcga accacgacgt
     3061 ggtgtcgcca agctccggca gccaatgcac cgtgctgacg atgacgatgc gatagccgag
     3121 gctgcgcaga tgccgcagca cttcgctgag caggcgttcg gcgccgccca gcacgaggaa
     3181 tgggactgcc agcagaatgg tacggcaccg cgcgtccttt ccggtgcttc gaaccatgtt
     3241 cctggctccg gcggtggagc gcatgcagac acgggcgctc tcccgggagc gcttgagcgc
     3301 ggccggcgtg accagtgcct gctcaagttc ctggatcagc ttcccctgca ccgcattggg
     3361 cagcatcccg ggcttgctgc tcagactggc attcgtatgc gcgcgataca ggaagagctg
     3421 ctcgccggtg atgttcgcga aacgcgcccc gtggcaggca acacgaatcc agaagcgcca
     3481 atcctcatgc aggtacacct gatcgggccg cgaatcttcg tagccgccgg ccaactccca
     3541 aaaggaccgg cggaacacgg cgcaggtggt gacgtgattg ccttcgagca ggtcggcgag
     3601 atcgggcgcg gggagcacgc cataggtttc ctccaccgca ccaaagcggc ggaccgcggt
     3661 ggagaccacg tcatatccat agtgttccaa caggaacacc gccttctcga tgtaggtcgg
     3721 atgcagcaga tcgtcggcgt cgaggcagca aacatacttt ccccgcgcac tgcgaatccc
     3781 gaaattgcgg ttctccccgg cgcggtgcgg ctcgtcgcgc agcagcaccg tcacgcgcgg
     3841 cgcctgaagc tgccgcacga tgtccggtgt tgtgccgtcc gttgatccgc cttcaacgac
     3901 aataacctcc agatcctgga aggtctgcgc gagcaccgag tcgaccgcct gcctgacgaa
     3961 agcaccgtag ttgaagcacg ggatgatcac gctgacgaga ggtcgatcgc gatgccattc
     4021 cacctcctcg tacgggtcgg gcgaggagga agacggcggc agcgcctcag gctgcgccgg
     4081 aattcccagg cgggagcggt aagaaatgtg cagccgcacc tcggtccaga acacgcgcaa
     4141 cccggcatgc cgcgccaact cccaggcttt gcgcagccgc ttgcggttca ctgtccgccg
     4201 caggacgcca agccgggagg cgcggttcag ccacagcgcg acggtcgagc ccaggccgcg
     4261 caatggcgcg cccgccacga gagagacagg cttcgcgctt tccggagcct gccgggtcgc
     4321 tcgttcagac atttgatgta tccgcaagat gcttactgcc cgttccccat cgctcggcca
     4381 gtcaggccgg ctgcaagcgt gagaagcggt ctgcgcaggg ttaacacgcc cgccatgcgt
     4441 ggtgcgatat cccggcaggc gcctgcgtca aatagccgta gcgcccatcg ggtgccggca
     4501 agccagatcc ggcgaagtgc caagcccgcg gcggatcgcc ctccagggcg ggctgcgccg
     4561 catcccgcgg cgtggatatt ccacgttgcc gcgcctcagc agatgagata ccagccgaga
     4621 tcatggggaa tgccgaagcc tatggtgaca gaccctggcc atcgcaggcg gttgaacccg
     4681 atatggcgca agccaaacac ctgatgagat gcgcttccga atgaacgtcc tggtttggca
     4741 gtggggacgc ttcggcggcg ccccgcgcgt ggctgtggcg cttgttcaag gcatgaagac
     4801 gctgccgaac gtcaacgtct cgctgtccat gtcgcgtaac gcggaattga tgcgggacgg
     4861 cagcatgcca ccctgcacgc tgccggtgga cacctatcgc ggtcggctgg gatttctctg
     4921 gcgcttcgcc accatccccg ttgcggcgcg gtctctcgcc cgcaggatcg cccggctacg
     4981 gccggatgtc gcaatctgtg cccagaccgg accgctcgat ctgctgatgg cgcgggcctt
     5041 gcgcctgctg ggcattccga tggtggtgtt gatccacgac gccgacctgc accccggcga
     5101 cggcaagttg tttcaaatgc gacttcagcg cgcgctgtgc cggcgcgccg ccgccctggg
     5161 cgcgctttcc gcccatgttg gcgcacgcct gaaggcgcaa ggcctggcag gcaacggcgc
     5221 gcggccgctg atccagctgc gcttgccacc actgccgatc gcggtccccg cggccgaacc
     5281 acccgccggc agcgggttcc gccttctcat cttcggacgg ctgctgccgt acaagggatt
     5341 ggacatgctc gccgaagcat tgagccgcct tgggccgctg ccgggtctga cggttcgcgt
     5401 ggtggggctt gggccggaaa gctccgaact gaagcagttg cgggcgatcc ccaacgtgac
     5461 cgtggaaaac cgctgggttc ccgaatcgga actgccgcag ttgctggcct gggcggatgc
     5521 cctggtgctg ccgtaccggg aggcaagtca aagcggcgtg gcagcactgg ccctgggtgc
     5581 cggccgccac gttctcgcga cacgtgtcgg cgggctgagc gagcagctcg gcaacgagcc
     5641 gctggcgatc ctgtgcgatc ccgacccggt cagcctgagc gaagcgctgc gcacactggt
     5701 tgcagccagt ccttcggcca gcacggcacc ggcggccgat cccatcgccg catggcgcga
     5761 catggccgag gaactgctcc ggcaaatcgc agcctgccgg ccggtccagg ctgatcgggt
     5821 cgcgcgctgt ggggcggtgg ttcccggcgg gctgaacctg aatggtgctg ctggtcagga
     5881 ttgaactgac gacctctccc ttaccaaggg agtgctctac cactgagcta cagcagcgcc
     5941 gggcggagaa gacggcccgt tcctagtcgt tgcccgggca cggtgcaagc ccgggaagcg
     6001 ccgctctgcg cgacagggcg aaagcccaac ccacgcgcac tggcgggaac agactgccgc
     6061 ctgtatgtgg ctgcacacca tgccgtcgcg ccatatcgcc cgtctggaaa ccgtcagtgt
     6121 caccgtgccg gatgcggcgc tggaagccta cgaagccgcg ctggacagca cctgcgacac
     6181 ggtcgggttc ttccgcgacg aggactccgg cgactggcgg gtggaggggg tgaagccggt
     6241 cggtgccggc gaagcggaac tcagcgccag tctggccctc gccgccggcc tgtccggttt
     6301 cgtcgcgcgg ctggagcgca gtgacacgcc tgccgaaggc tggctcgcgc gcagctatgc
     6361 gggcttcccg gagcaacgca tcggccagca cttcgcgatc cgcggcacgc acctgccgcc
     6421 ggagacggtg gcgggccgca tcacgttgct gctcgatgcc ggcctggctt tcgggtcggg
     6481 agaacacgga tcgacgcgcg gctgcctgct ggcgctggaa cgcgtggcac gccgggcgcc
     6541 gcggcgcatc ctggatatgg gcactggctc cggcattctg gcgatcgccg ccgcccggct
     6601 gctgcgccga ccggtactgg cgaccgatat cgagccctgg tcggtgcgtg tggcggggga
     6661 caacgcgagg ctgaacggcg tggcgcacct ggtgcgtcca ctgcgggcgg acggctggcg
     6721 tgatgcacgg gtgcgcgccg gcgggcccta tgatctggtg ttcgcgaaca tcctggctcg
     6781 gccgctctgc ctgatggcac acgaccttgc ggcgcagttg gccccgggcg gcaccgccat
     6841 cctggcgggg ctgctcagca cccaggcgcg cgccgttttg gcggcgcatc ggcggcaggg
     6901 cttgcggttg gagcggatga tcgtcgtggc cccctgggcc acgctggtgc ttcgccggac
     6961 cgcagtcgag cgcgcctgaa aggcgcgctc gtgctcactg cggaggtcga ggccccgaac
     7021 tcagccttcg ctgcgggcaa ccagatcggc gttgtgggcg ctgtcgaaca cgcgcgccat
     7081 gtcggcacgg ctcaggccga tatccatcag ttcgcggtcg ctcatcatct cgagttcggc
     7141 catcgcggac tgccgttccc gccaagcctg caaagcggcg atccgggccg ccagccagcg
     7201 gccaaagctc ggcttcggaa gctcggtcgg cagcgtgacg cggccgaggt tgggctcctc
     7261 cgggtgcgcg tccacataag tcaggttggg aagctggtag gcaagttggt ctttggcgat
     7321 cggggcactc atgggtctaa ttcccatctg tatccacccg ggtctggcgc ggcgtcgtcg
     7381 cggcggggca cccccgggtc tttctgcact gcaacataag cctgaacgca tgttgcgata
     7441 attgccaatt cgctcccgct gccttgcgct caacgcatgg atggttaacc agctatgaac
     7501 ccaccgatgg cctggcttga gaggaactct gcatgaacgg cacccgcctt gcgtccctcc
     7561 gcgccgaatt ggcccgccag ggcctggatg gcttcatcgt gccgcgggcc gacgagcatc
     7621 tgggcgaata cgtgccggcc gccgccgagc ggctggcctg gctgaccggg ttcaccggca
     7681 gcgccgggct cgccgcggtg ctgccggacc gcgccgcggt cttcaccgac ggccgctatg
     7741 tactgcagtt ggccgagcag accgatccga gcctgtggca acgcaagcat atcaccgagg
     7801 aacccccgcc cgcctggctc gccgcgcagg cccagcccgc cgcccgcatc ggctacgacc
     7861 cgctgctgat cagcgaggag gcgctcggcc gctacaccga agcgggattg aagatgcagc
     7921 cggtggcgca caatccggtt gacgcggtgt ggaccggccg cccggcgccg cccgccgcac
     7981 ccgccatgcc gcatgctctg gcctatgccg ggcgcagcgc ggaagacaag cggcaggaga
     8041 tcgccgcagc gctgcaagcg gcgaagcagg acgcggcggt gctgagcgat ccctcctcca
     8101 tcgcctggct gctgaatatc cgcggtggcg acgtgccctt cacgcccttc gccctcggct
     8161 tcgccctggt gcatgccgat ggcgggacgg aactcttcat ggatccggcc aagctgagcg
     8221 atgagacgcg cgcctggctg ggcaatgccg tggcggtcgc cgggcgcgag gcgctcggcc
     8281 cggcattgtc gcggctgggg ggcaagcggg tgcgggtcga tccgggcggc tcgccggtgt
     8341 ggttctccca aatgctgcgc gacgccggtg ccaccgtcgt cgccggcccg gacccctgcc
     8401 tgctgccgaa agcctgcaag aacccggtgg agcagcaagg cgcccgcaat gcccacgccc
     8461 gcgacgccgt ggccatggtg cgcttcctcc actggctgtc gcaggtcggc cctggcggtg
     8521 gggaaaccga gctgtccgcc gcggaccagt tgctggcgtt ccggcgcgag gtggacgggt
     8581 tccgcggtga gagcttcccg gcaatctccg gggccggcga gcatggcgcg gtgatccatt
     8641 accgggtcac gccggagagc aaccggccga tccggcccaa c
//
LOCUS       DASSZH010000475        11191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_931849, whole genome shotgun sequence.
ACCESSION   DASSZH010000475 DASSZH010000000
VERSION     DASSZH010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 11191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11191
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931849"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VFN42_10365"
     CDS             complement(<1..927)
                     /locus_tag="VFN42_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET8997056.1"
                     /translation="MPAPLLGQPAQPSVAVLIPCFNEEATIGKVVTDFRASLPHATIY
                     VYDNNSRDRTALEARAAGAVVRHEPLLGKGNVVRRMFADVQADCYFLVDGDDTYDATA
                     APAMLRLLLARQLDMVTAARVTQLQAAYPRGHRFGNRLLTALVRGVFGDRISDVLSGY
                     RVFSRRFVKSFPALADGFEIETEFTVHALEWRMPVDEVAAEYRMRPPGSQSKLHTLSD
                     GLRILRTILLLAKEGQPLRFFATAGTLLLVLAVACGAPVVMEFLETGLVPRLPSAVLA
                     TGLMSLSSLTLVCGLVLDSVERGRKEVKRLAYL"
     gene            complement(1018..1737)
                     /locus_tag="VFN42_10370"
     CDS             complement(1018..1737)
                     /locus_tag="VFN42_10370"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET8997057.1"
                     /translation="MLQARQSPIVFTPGALVLPALPQAVRFRHWAAAGNPISGWQAAV
                     KRGLDIAIALLTLTLFAVPLLLAAIAIKLDSPGPVLFRQHRIGLHNGGFELWKLRTMY
                     HRPPDRGPLRQATRNDPRVTRVGAWLRRVSLDEFPQFVQVLRGDMSLVGPRPHAPGTC
                     AGGVPFEQLSDRYAQRHRVRPGMTGLAQVRGWRGETDTPDKLLHRLDSDLEYIATWSL
                     RLDILVLVRTAACVLSMRNAW"
     gene            complement(1863..2318)
                     /locus_tag="VFN42_10375"
     CDS             complement(1863..2318)
                     /locus_tag="VFN42_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006558780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HET8997058.1"
                     /translation="MEALLLRLATQLPAPARTFATEARLRLLAQFAKFGTVGTLGFVV
                     DTGVVYGLRGAIGLYWAGLASYFVAATFTWIANRLWTFRGIGSGPAHRQWARFLGANT
                     LGFALNRGTYMLLITFVAICAREPVLATAAGAIAGMFVNFNMSRVLVFR"
     gene            2439..3278
                     /gene="dapD"
                     /locus_tag="VFN42_10380"
     CDS             2439..3278
                     /gene="dapD"
                     /locus_tag="VFN42_10380"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HET8997059.1"
                     /translation="MTEHRLQQPIDALWERRDTLSPATGGEARAVVDAALDAMDSGEL
                     RVAERGDQGWRVNQWLKKAVLLSFRLTDSGPMQGPGGAPVWDKVPMKFAGWGENRFRE
                     AGFRAVPGAVVRRSAYIAPGVVLMPSFVNAGAWVGRNTMVDTWATVGSCAQIGANCHL
                     SGGVGIGGVLEPLQANPVIIEDDCFIGARSEVAEGVVVERGAVLSMGVFIGASTKVID
                     RATGEIYLGRVPAYSVVVPGSLAGKPLPDGSPGPSLYCAVIVKTVDAQTRAKTSINEL
                     LRD"
     gene            3472..4590
                     /gene="dapE"
                     /locus_tag="VFN42_10385"
     CDS             3472..4590
                     /gene="dapE"
                     /locus_tag="VFN42_10385"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HET8997060.1"
                     /translation="MTDPLPLAQALIRCPSVTPADAGAQEVLAEALQRLGFAVTRLRY
                     GEIDNLFARIGTGGPHFCFAGHTDVVPAGAAAWTGDPFSAEVRDGVLYGRGACDMKGA
                     IAAFAAACAEHLQSGPPRGSISLLITGDEEGVATDGTVKVLEWMQANSQVPDFCLVGE
                     PTCPVRLGDMVKIGRRGSLNAKITMHGTQGHVAYPHRADNPVHRLVRALAALTAAPVD
                     AGSDWFQPSTLQLTSIDVGNPVTNVIPAAARAMLNIRFNDRHSGASLTAWLRATLEQY
                     AERFDLHVAISGESFLTEPGEQVNKLRDAIIRESGVEPKLDTGGGTSDARFISRYCPV
                     AEFGLVGATMHQADERVPVSELRDLARIYRGILAAFLP"
     gene            4587..5183
                     /locus_tag="VFN42_10390"
     CDS             4587..5183
                     /locus_tag="VFN42_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997061.1"
                     /translation="MTRIRPRPPLLPNRGSQGNILLGMLRVALGKADGMAQFGGTRHS
                     FLASLAPLVAFPLVGALLVLAGGKPLDALGELLGTLCVLLVPPVVSFELARLWRREDQ
                     WLHFATAFNWCYWLVPLLGMLLMFVVGLLTAAGLPEQVGVMVMVACLGGYGLWLHWFL
                     ALHGLHVSRLRAALLVVAINLVVAALVLGPRLLLMNRG"
     gene            5186..5749
                     /locus_tag="VFN42_10395"
     CDS             5186..5749
                     /locus_tag="VFN42_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997062.1"
                     /translation="MMGSVASGLQAALLFARGREDGLRYLEPDPGGVARSFWALPMAM
                     PAIVCLTLINWVQGGVPAHAGHALGLDMMAFVLGWILYAVLTYELVRYIGAVDRWPCF
                     IAAWNWCNVVENLLLVFGSIPALLGAPPIIAEASELFAIGWALWIEWFAIRSSLRANW
                     LVAAWLVVLDQAIGLTLATLGYRLMHL"
     gene            complement(5790..6674)
                     /gene="pstA"
                     /locus_tag="VFN42_10400"
     CDS             complement(5790..6674)
                     /gene="pstA"
                     /locus_tag="VFN42_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET8997063.1"
                     /translation="MSGHAAVQGSAIRRSQASPLLRRRRARTMLGWILCGAAFAGLAA
                     AMLWIILMVLIRGIGALTPTVFTDVTHGIAGGLLNAIEGTFVIAFGTLLLAVPFGIAA
                     GIYVSEFRHTVWARTIRFMSDVLVGVPSIVLGYAGYVTMVEWLGWHFSVAAASITLAV
                     LCLPYICRTTELALREVPDTLREAGYAMGAGPATVTFRLSMKTALPGVLTGVLLALAI
                     SVGETAPLLYTAGWSSYLWNGRLTHSPIGYLTYAIWAFINEPFASANELAYAAAFLTT
                     VFVLLVSLASRLVLMRRR"
     gene            complement(6671..7630)
                     /gene="pstC"
                     /locus_tag="VFN42_10405"
     CDS             complement(6671..7630)
                     /gene="pstC"
                     /locus_tag="VFN42_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET8997064.1"
                     /translation="MNFRRVVAMLGGVVPASMVAVLIFLTIYAWPAIVFNGAHFLWGM
                     NWNLGNTYADPIEIGGFTMLPGADYGILFLIGGTLLTTLIAMVIAVPVGVGAAIFLAE
                     AVPPGPRLWLSFLVELLAAIPSVVFGLWGYAVVVPLLGHYVFPLMASVLGWIPWFGEP
                     TGSGYGLLTSGIVLSLMIVPLITATLRDAIDSQPVAIRESAAAIGATKFETVWRVLMP
                     GLRSVLIGACVLATGRALGETMAVLMVSGNALNALPGTIYAPVSTMAAFIASQLDSAL
                     QDPTGLAVKSLAEVALVLCIISLGVNAAARAILAGVNARSARI"
     gene            complement(7695..8771)
                     /gene="pstS"
                     /locus_tag="VFN42_10410"
     CDS             complement(7695..8771)
                     /gene="pstS"
                     /locus_tag="VFN42_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET8997065.1"
                     /translation="MQVRLKSRWSIPAMVAAAALTFGAATTTMAAEAGHLLETGSSLL
                     YPLFNLWVPVYTKAHPGIQITTQSTGSGTGISQAISGVAQIGASDAYLSNALMKQHPD
                     MLNIPTAISSQMVNYNLPGLNEEHLKLSGPVLAGIYSGHITKWDDAAIAKLNPGAKLP
                     AKTIVPIHRTDGSGDTFIFTQFLSDSTPSWGKSIAYGTTVSWPAVPGALGAEGNPGMV
                     NALKQTPYSVAYIGVSFKKATDDAKMGEAMLENRAGKFVLPTVKNVQAAAAAMVAKTP
                     KDERISLIFAPGDDAYPIINYEYAIIKSDQPNKQVAMQVKDFLNWAISPQGGNAEHFM
                     QAVSFVPLPASVSKLSAENIAKIH"
     gene            complement(8998..10113)
                     /locus_tag="VFN42_10415"
     CDS             complement(8998..10113)
                     /locus_tag="VFN42_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014106456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET8997066.1"
                     /translation="MRRMLLTAALLSLPVAAMASEPIQGLYIGAAAGANFMQSFRTHV
                     YTPPGLGGGASGNIVGNTGFAGVGSVGWGFGNGLRAEIEGDFRSNGIRHFQSPGLPTA
                     ASGSVRNYGVMANVLYDVNLPYFSSIPFMSGFQPYIGAGAGYMWTHLNDVSFSNPAHS
                     FAVDGSSGKFAWQAIAGLGYQIPSMPNLTLTLEYRYLDITGGGHIAGATRVGGAIIPA
                     TYDVGAQHDHSIMVGLRWAFNTAAPPPPAPAPTPAAAPAPAVQPSRSYLVFFDWDKAN
                     LTPRARQIIHEAADNSTHVQYTQIQVNGYTDTSGSAQYNQRLSVRRADAVASELVRDG
                     VPRNAISIQGFGETHLLVPTGEGVREPQNRRVEIIIH"
     gene            complement(10304..>11191)
                     /gene="murA"
                     /locus_tag="VFN42_10420"
     CDS             complement(10304..>11191)
                     /gene="murA"
                     /locus_tag="VFN42_10420"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET8997067.1"
                     /translation="DLHLKGLEQMGARIELAGGYIDARVDGRLRGATIVFPFVSVGAT
                     ENLLMAAALADGQTVLANAAREPEISDLAECLVKMGARIEGIGTGKLTIEGVDALHGA
                     EHSIVPDRIETGTYACAAAVSGGSLFLRGARLEHLGAVVRTLREAGVEIADEPGGVLV
                     RRLNGLHGTDVMTEAYPGFPTDMQAQFMTLMSVASGAAMVTETIFENRFMHVPELNRM
                     GARINVHGASAIVRGVPHLSGAPVMATDLRASVSLVLAGLAAEGETVVNRVYHLDRGY
                     EALEQKLAACGADIERMTG"
BASE COUNT         1930 a         3699 c         3786 g         1776 t
ORIGIN      
        1 aagataggcc agccgcttca cctccttccg gccgcgttcg accgaatcca gtaccagccc
       61 gcagaccagg gtcagcgacg acagcgacat caagccggtt gccagaacag cgctgggcaa
      121 gcgcggcact aggccggttt ccaggaattc catcactacc ggcgcgccgc aggccaccgc
      181 caggaccagc agcagagtcc ctgcggtggc gaagaagcgc agtggctgcc cctccttcgc
      241 cagcagcagg atggtgcgca ggatgcgcag cccgtccgac agcgtatgca gtttagactg
      301 cgatcccggc ggacgcatgc gatattccgc cgccacttca tccaccggca tgcgccattc
      361 cagcgcgtgc acggtgaact cggtctcgat ctcgaagccg tcggccaaag cgggaaacga
      421 cttgacgaag cggcgggaga atacgcggta accggacagc acgtcgctga tccggtcgcc
      481 gaacaccccg cgcaccagag cggtcagcag gcggttgccg aagcggtggc cgcgcggata
      541 cgccgcctgg agctgcgtca cccgtgcggc ggtgaccatg tccaactggc gtgccagcag
      601 caggcgcagc atcgccggtg cagcagtggc atcgtaggtg tcgtcgccgt ccaccaggaa
      661 atagcagtcg gcctgcacgt cggcgaacat acgccgcacc acgttgcctt tgccgagcag
      721 cggttcgtgc cgcaccaccg cgccggcggc acgcgcctcc agcgccgtgc ggtcgcgcga
      781 gttgttgtcg tatacgtaga tcgtcgcgtg cggcaggctg gcgcggaaat cggtgacgac
      841 cttgccgatg gtggcttcct cgttgaagca agggatcagc acggcgacac tcggctgcgc
      901 cggctggccc aggagcggcg cgggcagcgg gagcggatcg gcggtccagc cggcacgcag
      961 cttcggcagg gcggcggcgg tggcgagctc gtccagcacg gtgtccacga cgctgcctca
     1021 ccacgcattg cgcattgaca gcacgcacgc cgcggtacgc accagcacca gaatgtccag
     1081 gcgaagcgac caggtggcga tgtattccag gtcgctgtcc agccgatgca acagcttgtc
     1141 cggggtgtcg gtctcccccc gccagccgcg cacctgcgcc aggccggtca tgcccggacg
     1201 cacgcggtga cgctgggcgt agcggtcact cagctgttcg aacgggacgc cgccggcgca
     1261 ggtacccggt gcatgcgggc gcgggccgac caacgacata tcgccgcgca gcacctgcac
     1321 gaactggggg aactcatcca gggaaacccg gcgcagccag gcacccacgc gggtgacgcg
     1381 cggatcgttc cgcgtggcct ggcgcagcgg tccccgatcc ggcggacggt ggtacatggt
     1441 gcgcagcttc cacagctcga acccgccgtt gtgcaggccg atgcgatgct ggcggaacag
     1501 caccgggccg gggctgtcca gcttgatcgc gatggcggcc agcagcagcg gcacggcgaa
     1561 cagcgtcagc gtcagcaggg cgatggcgat gtccagaccg cgtttgaccg cggcctgcca
     1621 gcccgagatc gggtttcccg cggcggccca gtgtcggaag cgaaccgcct gcggcagggc
     1681 cggcagcacc aacgcgccgg gcgtgaacac gatgggggat tgccgagctt gcagcatggc
     1741 cgcggcccat cctggctgtg gtcccgcgaa ggcgggaccg ttcgcacaag ctgtgacgcg
     1801 gaaggatcac cagccggtta acacggcggc aaaattcggt ggaaaaaccc gggtgttgcc
     1861 ggtcagcgga agaccagcac gcgggacatg ttgaagttga cgaacatgcc ggcgatggcg
     1921 ccggccgcag tcgccaggac cggctcgcgg gcgcagatcg caacgaaggt gatcagcagc
     1981 atgtatgtgc cgcggttcag cgcaaagccc agcgtattcg cccccaggaa gcgcgcccac
     2041 tggcggtgcg ccgggccgga gccgatgccg cggaacgtcc acagccggtt ggcgatccag
     2101 gtgaacgtgg cggcgacaaa ataggatgcc aacccggccc agtacagccc gatcgctcca
     2161 cgcaatccat aaaccacgcc ggtatccacc acgaagccga gcgtcccgac cgtgccaaat
     2221 ttggcgaact gcgccagcag gcgcagccgc gcctcggtgg cgaaggtacg cgcgggggcc
     2281 ggcaactggg tggccaaccg aagcagcagg gcttccatgc ggcggggcaa tagccaaacg
     2341 cggggaatcc tgcaacgagc gattgggcca cgggtcgcgt tgacaggatg cggcgtgctg
     2401 cgcatcgtcc ggcccgctcc agcagggacc gccacaccat gaccgaacac cgactgcaac
     2461 agccgatcga tgccctatgg gagcggcggg atacgctgtc acccgcgacc ggaggcgagg
     2521 cacgcgcggt ggtggatgcg gcgctggacg cgatggattc cggcgagctg cgggtggccg
     2581 aacgcggcga ccagggctgg cgggtgaacc agtggctgaa gaaggcggtg ctgctgtcat
     2641 tccggctgac cgattcgggg ccgatgcaag ggcccggcgg cgccccggta tgggacaaag
     2701 tgcccatgaa attcgccggc tggggcgaga accgtttccg tgaggccggc ttccgcgccg
     2761 tgccgggcgc ggtggtccgg cgctcggcct atatcgcgcc gggcgtggtg ctgatgccca
     2821 gcttcgtcaa tgccggcgcc tgggtggggc ggaacaccat ggtggacacc tgggccacgg
     2881 tcggcagttg cgcgcagatt ggcgccaact gccacctgtc cggcggcgtg ggcatcggcg
     2941 gcgtgctgga gccgctgcag gccaatccgg tgatcatcga ggacgactgc ttcatcggcg
     3001 cccgctccga agtcgccgag ggcgtggtgg tggagcgcgg cgcggtgctg tccatggggg
     3061 tgttcattgg cgcgtccacc aaggtgatcg accgggcgac cggagagatc tacctgggcc
     3121 gggtgccggc ctactccgtg gtcgtgcccg gctcgctggc gggcaagccg ctgccggacg
     3181 gctcgcccgg gccgtcgctg tattgcgcgg tgatcgtgaa gacggttgat gcgcagaccc
     3241 gggcgaagac ctcgatcaac gaattgctgc gcgattgagc gggagcagta aggtgctggg
     3301 agccaagact cctccctccc cccttgtggg ggagggatgg ggtgaggggt cgtgcgtaca
     3361 gccccgatct gccggatgga tcgcacgacc ccccacccta accctccccc acaagggggg
     3421 agggaattgc ctctcttccc tctttgcccc tcctgcgccg gccgcagccg aatgactgat
     3481 cccctgcccc tcgcccaggc gctgatccgg tgcccgtcgg tcacccccgc ggatgccggc
     3541 gcgcaggagg tactggcgga agcgctgcag cgcctcggct tcgccgtcac ccgcctgcgc
     3601 tacggcgaga tcgacaacct gttcgcccgg atcggcaccg gcggcccgca tttctgtttc
     3661 gcgggccaca ccgatgtggt gcccgccggc gccgccgcct ggaccggcga tccattctcc
     3721 gccgaggtac gcgacggcgt gttgtacggc cggggcgcct gcgacatgaa gggcgccatc
     3781 gccgcttttg ccgccgcctg tgccgagcat ctgcagtcgg ggccgccccg cggctcgatc
     3841 agcctgctga tcaccggtga cgaggaaggt gtcgccaccg acggcacggt caaggtgctg
     3901 gagtggatgc aggccaacag ccaggtgcct gatttctgcc tggtcggcga acccacctgc
     3961 ccggtccgcc tcggcgacat ggtcaagatt ggccgacgcg gcagcctgaa cgccaagatc
     4021 accatgcatg gcacgcaggg acacgtcgcc tacccgcatc gcgccgataa cccggtgcat
     4081 cggctggtgc gcgccctggc ggcactgacc gccgcaccgg tggacgccgg cagcgactgg
     4141 ttccagccat ccacgctgca gctcacctcc atcgacgtcg gcaatccggt gaccaacgtc
     4201 atcccggccg ccgcccgtgc catgctgaac atccggttca acgaccggca cagcggcgcc
     4261 tcgctgaccg cctggctgcg cgccaccctg gaacaatacg ccgaacggtt cgacctgcac
     4321 gtggcgatca gcggcgagtc cttcctgacc gaaccgggcg agcaggtgaa caagctgcgc
     4381 gacgccatca tccgcgaatc aggcgtggag ccgaagctgg ataccggcgg cggcacctcc
     4441 gacgcgcgct tcatttcccg ctactgcccg gtggctgagt tcggcctggt cggggccacc
     4501 atgcaccagg cggacgagcg ggtgccggtt tcggaactgc gcgacctggc gcggatttat
     4561 cgtgggattc tggcggcctt cctaccgtga cccgaatccg accgcgacct ccgctgctgc
     4621 ccaatcgcgg cagccagggc aacatcctgc tgggcatgct gcgcgttgcg ctcggcaaag
     4681 ctgacggcat ggcgcagttc ggcggcacac ggcactcctt cctggccagc ctggcaccat
     4741 tggtcgcctt cccactggtc ggcgcactgc tggtactggc gggcggcaag ccgctggacg
     4801 ccctgggcga gttgctgggc acgctgtgcg tgctgctggt gccgccggtg gtatcgtttg
     4861 agctggcgcg gctgtggcgg cgcgaagacc agtggctgca cttcgccacc gccttcaact
     4921 ggtgctactg gctggtcccg ctgctcggca tgctgctgat gttcgtggtc gggttactca
     4981 cggcggcggg gttgccggag caggtcggcg tgatggtcat ggtggcttgc ctcggcggct
     5041 atgggttatg gctgcactgg ttcctggcgc tgcacgggct gcacgtgtcc cggctgcgcg
     5101 cggcgctgtt ggtggtggcg atcaacctgg tcgtcgcggc tctggtactg ggcccgcgcc
     5161 tgctgctgat gaatcgaggt tgaacgtgat gggatcggtg gcatccgggc tgcaggccgc
     5221 cctgctgttt gcccgcgggc gcgaggacgg gctgcgctat ctcgaaccgg accccggcgg
     5281 ggtggcgcgc agcttctggg cgctgccgat ggcgatgccg gcgatcgtgt gcctgacgct
     5341 gatcaactgg gtgcagggcg gcgttccggc gcatgcagga catgcgctgg gcctcgacat
     5401 gatggccttc gtgctcggct ggatcctcta tgccgtgctg acctatgaat tggtccgcta
     5461 catcggcgct gtcgaccgct ggccgtgctt catcgcggcg tggaactggt gcaatgtggt
     5521 ggagaacctg ctgctggtgt tcggcagcat ccccgcattg ctgggtgcgc cgccgatcat
     5581 cgccgaggcg tcggaactgt tcgcgatcgg ctgggcgctg tggatcgaat ggttcgccat
     5641 ccgatcctcc ttgcgcgcca actggctggt ggccgcctgg ctggtggtgc tggaccaggc
     5701 gatcgggctg acgctggcga cgctgggtta ccggctgatg cacctgtagc cgcccgacca
     5761 ctcaagaccg ggcggaacgc cggcctctgt caccgccgcc gcatcagcac cagccgcgag
     5821 gccaggctga ccagcagcac gaacaccgtg gtcaggaatg ccgccgcata ggccagctcg
     5881 ttggcggacg cgaagggctc gttgatgaac gcccaaatcg cgtaggtcag atagccgatc
     5941 ggcgaatggg tcagcctgcc gttccacaaa tagctcgacc agccggcggt gtacagcagt
     6001 ggtgcggtct ctcccaccga gattgccagc gccagcagca cgccggtcaa tacgccgggc
     6061 agcgcggtct tcatgctcag ccggaaggtc accgtggccg gtccggcacc catggcgtag
     6121 ccggcctcac gcaacgtatc cggcacctcg cgcaatgcca gttccgtggt acggcagata
     6181 tacggcaggc acagcaccgc cagcgtgatg ctggccgcgg cgaccgagaa gtgccagcca
     6241 agccattcca ccatggtgac gtaaccggca tagcccagca cgatggaagg gacaccgacc
     6301 agcacgtcgg acatgaagcg tatggtgcgc gcccatacgg tgtggcggaa ctcggagacg
     6361 tagatacccg ccgcgatgcc gaagggcacc gccaggagca gcgtgccgaa ggcgatgacg
     6421 aacgtaccct cgatggcgtt cagcagaccg ccggcgatgc cgtgggtcac gtcggtgaac
     6481 accgttggtg tcagggcgcc gataccgcgg atcagcacca tcaggatgat ccacaacatc
     6541 gccgccgcga ggccagcgaa cgcggcaccg cacagaatcc agccgagcat ggtacgcgcg
     6601 cggcggcggc gcagcagcgg cgatgcctgg ctgcgccgga tcgcgctacc ctgaaccgcg
     6661 gcgtgcccgc tcatatccgt gcgctccgcg cgttgacgcc tgccaggatc gcacgagcgg
     6721 cggcgttcac gccgagcgag atgatgcaca gcaccagcgc cacttcggcc agcgacttga
     6781 ctgccaggcc agtggggtcc tgcaacgcgc tgtcgagttg cgacgcaatg aaggcggcca
     6841 tggtggagac cggcgcatag atcgtgcccg gcaacgcgtt cagcgcatta ccgctgacca
     6901 tcagcaccgc catcgtctca cccagcgcgc ggcctgtcgc cagaacgcag gcaccgatca
     6961 gcaccgagcg caagcccggc atcagcacgc gccagacggt ctcgaacttg gtggcgccga
     7021 tcgccgcggc cgattcgcgg atcgccaccg gctggctgtc gatcgcgtcg cgcagtgtcg
     7081 cggtgatcag cggcacgatc atcagcgaca gcacgatgcc ggaggtcagc aagccgtagc
     7141 cgctgccggt gggttcgccg aaccagggaa tccaacccag caccgaggcc atcaggggaa
     7201 atacgtagtg acccagcagc ggcaccacca cggcatagcc ccacaggccg aataccacgc
     7261 tgggaatcgc tgccagcaac tccaccagga aagacagcca cagccgcggt cctggcggca
     7321 ccgcctcggc caggaagatg gcggcgccga cgccgaccgg cacggcgatg accatcgcga
     7381 tcaatgtggt cagcagcgtg ccgccaatca ggaacaggat gccgtagtcg gcaccgggca
     7441 gcatggtgaa gccaccgatc tcgatcggat cggcataggt gttgcccagg ttccagttca
     7501 tgccccacag gaagtgggcg ccattgaaca cgatcgccgg ccacgcatag atcgtcagga
     7561 agatcagaac ggcgaccatg ctggccggca cgacgccgcc cagcatggct actactcgac
     7621 ggaaattcat gcggaagccc cgtcccggaa tcgcctggat cgtagcacgg ctggccggga
     7681 cgggttgagc cccatcagtg gatcttcgcg atgttttcgg cgctcagctt gctgacgctc
     7741 gccggcagcg gcacgaaact gaccgcctgc atgaagtgct ccgcattgcc gccctgcggg
     7801 ctgatcgccc agttcaggaa gtccttcacc tgcatggcga cttgcttgtt cggctgatcc
     7861 gacttgatga tggcatactc gtagttgatg atcggatacg cgtcgtcgcc aggggcgaag
     7921 atcaggctga tccgctcatc cttcggggtc ttggccacca tggcggcggc ggcagcctgg
     7981 acgttcttca ccgtcggcag cacgaacttg ccggcgcggt tctccagcat cgcctcgccc
     8041 atcttcgcgt cgtcggtggc cttcttgaag ctgacgccga tataggcaac cgagtacggg
     8101 gtctgcttca gcgcattgac catgcccgga ttgccttccg cgccgagcgc acccggcacg
     8161 gccggccagc tcaccgtggt gccgtaggcg atgctcttgc cccaggacgg ggtgctgtcg
     8221 ctcaggaact gggtgaagat gaaggtgtcg ccactgccgt cggtccgatg gatcggcacg
     8281 atggtcttgg ccggcagctt tgcacccggg ttcagcttgg cgatcgcggc gtcatcccac
     8341 ttggtaatgt ggccgctata gatgcccgcc agcaccggac cgctgagctt caggtgctcc
     8401 tcgttcaggc cgggcaggtt gtaattgacc atctggctgg agatcgcggt cgggatgttc
     8461 agcatgtccg gatgctgctt catcagcgcg ttgctgagat aggcatcgga cgcgccgatc
     8521 tgcgccaccc cggagatggc ctgcgagatg cccgtgccac tgccggtgct ctgggtggtg
     8581 atctgaatgc cgggatgggc cttggtgtaa accgggaccc acaggttgaa caacgggtag
     8641 agcaggctgg aaccggtctc gagcaagtgg ccggcttccg ctgccatggt cgtcgtggcg
     8701 gcaccgaaag tcagtgccgc ggctgcaacc atggcgggta tcgaccaccg ggactttagg
     8761 cgaacctgca ttgcgtcctc cgcatgaaaa acaaaagcgc gcgatagcta gtcgaggcgt
     8821 tggcggcgcg ccacacggcg gagggccgaa aactgcatct tcatggaagt gtcaaaatct
     8881 gcggatgctt gcccggcggc aaatcccggg ccggagcggc ggtccttccc ggccccggac
     8941 agcatcatgg ccggcactgg gccggccatg agcttggcaa cgggtacgag acggcagtta
     9001 gtggatgatg atctccacac gccggttctg cggctcacgc acgccttcgc cggtcggcac
     9061 cagcagatgc gtctcgccga agccctggat ggagatcgcg ttgcgcggca cgccatcccg
     9121 caccagttcc gacgccacgg catccgcccg gcggaccgaa aggcgctggt tgtactgggc
     9181 cgaacccgag gtgtcggtgt agccgttcac ctggatctgc gtgtactgga catgggtgga
     9241 gttgtcggcc gcctcgtgga tgatctggcg ggcacgcggc gtcaggttcg ccttgtccca
     9301 atcaaagaac accaggtacg agcgggacgg ctgcaccgcc ggggccggcg cggcggccgg
     9361 cgtcggggca ggcgcgggcg gcggcggggc ggccgtattg aacgcccagc gcaggcccac
     9421 catgatggaa tggtcgtgct gcgcgccgac atcataggtg gccgggatga tggcgccgcc
     9481 cacacgcgtc gcgccggcga tatgcccgcc accggtgatg tcgagatagc gatattccag
     9541 cgtgagagtc aggttcggca tgctggggat ctggtagcca agcccggcga tggcctgcca
     9601 ggcaaacttc ccgctgcttc cgtcgaccgc gaaggaatgt gccgggttgc tgaaggacac
     9661 gtcgttcagg tgggtccaca tgtagccggc accggcgccg atatagggct ggaatccgga
     9721 catgaaggga atgctggaga agtagggaag attgacgtca tacaggacgt tcgccatcac
     9781 gccgtagttc cgcacactgc cggatgcggc ggtgggcagg ccggggctct ggaaatgccg
     9841 gatgccattg ctgcggaaat cgccttcgat ttcggcgcgc agtccattgc cgaagcccca
     9901 gccgacacta cccacgccgg cgaagccggt gtttccgaca atgttgccgc tggcgccgcc
     9961 gcccagacct ggcggtgtgt acacatgcgt gcggaagctc tgcatgaaat tggcacccgc
    10021 agcagcgccg atatacagac cttgaatggg ttcggaggcc atggcggcca ccggcaggct
    10081 aagcaaagca gcggtgagaa gcatccggcg catcgcgact tgtctcctgt tcatgacgtg
    10141 ccgggactat ttgcaacacg cggagaagcg gccaaatcac aaaaagttag gtgtggcaga
    10201 gccgccacaa agcagcggat gcaacggtaa cattccagct accaacaggg tttttgcaag
    10261 ccgcgatgat gcggcaatgg taccggaacg cgccgagcgc ggttcagccg gtcatccgct
    10321 cgatatccgc accacaggcg gccagcttct gctccaacgc ctcataaccg cgatcaaggt
    10381 gatacacgcg gttcaccacc gtctcgcctt ccgccgccag gccagccagc accagcgata
    10441 ccgacgcgcg caggtcggtc gccatgaccg gcgcacccga cagatgcggc acgccgcgca
    10501 cgatcgcgct ggcaccgtgc acgttgatgc gggcacccat gcgattcagt tcaggaacgt
    10561 gcatgaagcg gttctcgaag atggtttcag tcaccatcgc tgccccggat gcaaccgaca
    10621 tcagcgtcat gaactgcgcc tgcatgtcgg tgggaaaccc gggataggct tcggtcatta
    10681 cgtcggtccc gtgcagcccg ttcagccggc gcaccagcac accgccgggc tcatcggcga
    10741 tctccacccc tgcttcgcgc agcgtgcgca ccacggcgcc gagatgctcc agccgggcgc
    10801 ctcgcaggaa caggctgccg ccggacaccg ccgccgcgca agcataggta ccggtctcga
    10861 tccgatccgg cacgatgctg tgttccgcgc catgcagcgc gtccacgccc tcgatggtca
    10921 atttgccggt accgattccc tcgatgcgcg cgcccatctt caccaggcat tccgccaggt
    10981 cgctgatctc cggttcgcgc gccgcattgg ccagcacggt ctggccatcc gccagcgccg
    11041 cggccatcag caggttctcc gtggcgccga cggaaacgaa aggaaagacg atggtggcgc
    11101 cgcgcagccg gccgtccacc cgcgcatcga tatagccgcc cgccagttcg atgcgggcac
    11161 ccatctgctc cagccccttc aggtgcagat c
//
LOCUS       DASSZH010000476         2093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_727397, whole genome shotgun sequence.
ACCESSION   DASSZH010000476 DASSZH010000000
VERSION     DASSZH010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727397"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VFN42_10425"
     CDS             <1..654
                     /locus_tag="VFN42_10425"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HET8997068.1"
                     /translation="DLSNAAFPYLAAGTLPLADGGEGRLFRVSYSGECAFELAVPARD
                     GERVLRSIMQAGAAFGIMPYGTEALGMLRIEKGHVAGGELNGQTTARDLGLERLRSRK
                     KDFVGRVLAQRPALLAPERPSLVGLRPVDRQDRFSAGAHFLQPAASERAENDLGWVSS
                     AAFSPTLGHQIGLGLLAGGLGRIGQHLRAFDPVRGSDVMVEVVHPCFIDPEGERLRV"
     gene            647..1168
                     /locus_tag="VFN42_10430"
     CDS             647..1168
                     /locus_tag="VFN42_10430"
                     /inference="COORDINATES: protein motif:HMM:NF016180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HET8997069.1"
                     /translation="MSDVRSRLALTVCESVSLAMILLRRGTASAASSALQARCGFALP
                     QPGRLTSASATQVLWSGPDRFLAVRDDPRTGLAAELTAALGDLAHVVEASSSRTVFEV
                     AGSGAAEALNRVLPIDLHPRAFPAGSVALTSAAHMAVQLWRPGDDAVFRLACSSSFAA
                     SLRHHLAAAGFGD"
     gene            1348..>2093
                     /locus_tag="VFN42_10435"
     CDS             1348..>2093
                     /locus_tag="VFN42_10435"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8997070.1"
                     /translation="MIKRFHMLYVGQIDLDNVGLHGTPANERRYPNERLSEVFFTARD
                     VARLMDDLGYYAFWTAEHHFQREGYECLPNLIQLGLWLATQTRRIKFGCGFNVLPMWH
                     PIRLAEDYAMADIVTDGRIIMGVGRGYHTREVETFGAPLIDADRNREYFEEQLQLLLT
                     CFNEEQFHFKGRYFECPPQVDYRGYKLTDITMVPRPKHLPVEVFMPVASGRTIDMMAR
                     YGLKAMVTLNGEKILDDVVRAYQSACAAHG"
BASE COUNT          306 a          698 c          722 g          367 t
ORIGIN      
        1 gatctgtcga acgcggcctt cccctatctg gcggccggca cgctgcctct ggcggatggc
       61 ggcgaggggc ggctgttccg cgtgtcctat tccggcgagt gcgccttcga actggcggtg
      121 cccgcgcgtg acggggagcg cgtgctgcgc agcatcatgc aggctggcgc cgcgttcggc
      181 atcatgccgt acggcaccga agcgttgggc atgctgcgca tcgaaaaagg ccacgtcgcc
      241 ggcggcgaac tcaacggcca gaccaccgcg cgcgacctgg gcctggagcg gctgcggtcg
      301 cgcaaaaagg acttcgtcgg ccgcgtcctg gcgcagcgtc cggcgttgct ggcgccggag
      361 cgtccgtctt tggtcgggct gcgcccggtg gaccggcagg accgcttctc ggcgggcgcg
      421 catttcctgc aaccggcggc gtccgagcgg gccgaaaacg accttggctg ggtcagttcc
      481 gcggcgtttt cccccactct cgggcaccag atcgggttgg gcctgctcgc gggcgggctg
      541 ggccggatcg gccagcacct gcgcgccttc gatccggtgc gtgggtcgga cgtgatggtg
      601 gaagtggtgc atccctgctt catcgatccg gaaggagaac gcctgcgtgt ctgacgttcg
      661 ctccaggctg gcgctcactg tttgcgagtc ggtgtcgctg gcgatgatcc tgctgcgccg
      721 tggcaccgcg tcggcggcgt ccagcgcctt gcaggcgcgg tgcggcttcg cgttgccgca
      781 gccgggccgg ctgacatccg cgtcggccac gcaggttcta tggagcggcc ccgaccgctt
      841 cctggcggtg cgcgacgacc cgcgcaccgg gctggcggcg gagctgaccg cggcactcgg
      901 cgatctggcg catgtggtgg aggcgtcgtc ctcgcgtacg gtgttcgagg ttgccggcag
      961 tggcgccgcc gaagcgctga accgcgtgct gccgatcgat ctgcacccgc gtgcgtttcc
     1021 ggcgggaagc gtggcgctga ccagtgcggc gcacatggcc gtgcagctct ggcggcccgg
     1081 cgatgacgcg gtgttccgcc tggcctgcag cagcagtttt gccgcgagcc tgcgccacca
     1141 cctcgcggcg gccggcttcg gggattgaac cgctgcgtgt catggccggg cttggcccgg
     1201 ccacccacgt tttgtctggc ttgccgccga taaggacgtg gccaagcccg ggcatgacac
     1261 ggagcagagc gtccgcgcgt ccccaccccc gcttgacctg cctccccggg cggtgcgtgc
     1321 ttcccgcaac cgaacgggag gcacgtcatg atcaagcgtt tccacatgct gtatgtcggc
     1381 cagatcgacc tcgacaacgt cggactgcac ggcacgccgg cgaacgaacg ccgctatccc
     1441 aacgaacgtc tgagcgaagt gttcttcacc gcgcgcgacg tcgcgcggct gatggacgac
     1501 ctcggctatt acgcgttctg gaccgccgag caccacttcc agcgcgaagg ctacgagtgc
     1561 ctgccgaacc tgattcagct cggcctctgg ctcgccacgc agaccaggcg gatcaagttc
     1621 ggctgcggct tcaacgtgct gccgatgtgg catccgatcc gcctggcgga agattacgcc
     1681 atggcggata tcgtcaccga cggccgcatc atcatgggcg tcggccgcgg ctaccacacg
     1741 cgggaggtgg aaaccttcgg cgcaccgctg atcgatgccg acaggaaccg cgaatatttc
     1801 gaggagcagc ttcagcttct gctcacctgc ttcaatgagg agcagttcca cttcaagggc
     1861 aggtatttcg aatgtccgcc gcaggtggat taccgcggct ataagctcac cgacatcacg
     1921 atggtgccgc ggccgaagca ccttccggtg gaagtgttca tgccggtcgc cagcggccgc
     1981 accatcgaca tgatggcgcg ctatggcctg aaggctatgg tgacgctgaa cggcgagaaa
     2041 atcctcgacg acgtggtgcg tgcttatcaa tccgcctgtg ccgcgcacgg cca
//
LOCUS       DASSZH010000477         2280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_795501, whole genome shotgun sequence.
ACCESSION   DASSZH010000477 DASSZH010000000
VERSION     DASSZH010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795501"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VFN42_10440"
     CDS             complement(<1..244)
                     /locus_tag="VFN42_10440"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET8997071.1"
                     /translation="MAKPEHDARRTALLGSPLFQAMQPEELNEILSLALERRFKRGQT
                     IFQKDDSGSSMMAVLRGLVRISVVSAEGKEITLNVIR"
     gene            complement(346..870)
                     /gene="pal"
                     /locus_tag="VFN42_10445"
     CDS             complement(346..870)
                     /gene="pal"
                     /locus_tag="VFN42_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019089103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HET8997072.1"
                     /translation="MFRQVICAFAVVGLLAGCASDEGASNGAGGAYGQQGAGAPGAGA
                     YGANGYGAGQAAPGSQEDLVQTVGDRIFFATDRSSLDDQARQTLDRQAAWLQRYPQVN
                     VVIAGNCDERGTEEYNLALGQRRAATDRDYLVAHGVAANRIQTISYGKDRPTDAASTP
                     EAWAKNRNAITYVR"
     gene            1056..1604
                     /locus_tag="VFN42_10450"
     CDS             1056..1604
                     /locus_tag="VFN42_10450"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET8997073.1"
                     /translation="MARVPYLDVADLPPEEQDLLKRPIALHKALVNSPKGARAFAALG
                     QFIRYGSKLDPRLRELAILQVGWLARSPYEWSHHIKIGHDFGVTDADIRALIEESAGR
                     ATQLDALTRTVLRAAREMTGQGAMSDASFAALQAALGHEQVVDLTLTIGFYNAVVRVL
                     ATLQIDVEPEYAPYLQQFPLPQ"
     gene            1746..>2280
                     /locus_tag="VFN42_10455"
     CDS             1746..>2280
                     /locus_tag="VFN42_10455"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET8997074.1"
                     /translation="MTFDSEAVLDAMPDPFYAVGPDWRLLYVHRAAECVWNVRRAEVI
                     GRGLLEVFPALAGSESHRAMQQAMATGEPRRLEAVSSVLGLPFEVDLNPMAWGLAIHV
                     HDLTARKRMEGQLRERGELLTLAEQSAGVGVWEIDIASDTVRGTPQYFRIMGLEPTAD
                     PVPMARIRALRMPEDHDA"
BASE COUNT          343 a          770 c          776 g          391 t
ORIGIN      
        1 gtcggatgac gttcagcgtg atctccttgc cctcggctga gaccacgctg atgcgcacca
       61 agccgcgcag caccgccatc atcgaggagc cgctgtcgtc cttctggaag atggtctggc
      121 cgcgcttgaa ccgccgctcc agggcaagcg acaggatctc gttcagctcc tccggctgca
      181 ttgcctggaa cagcgggctg cccagcagcg ccgtacgccg cgcatcatgc tccggctttg
      241 ccatcgttcg gtccccgttc ccgccggttg ccccaggtgg ggcagggcgg ttgagcgcca
      301 tagtcgcgcg cggctgccgg aggaggcaac gccccggcgc ccgggtcagc gcacgtaggt
      361 gatggcgttc cggttcttcg cccaggcttc cggggtggag gccgcatcgg tcggccgatc
      421 cttgccgtag ctgatcgtct ggatgcgatt ggcagcgaca ccatgcgcca cgaggtagtc
      481 gcggtcggtg gcggcgcggc gttggccgag ggcgaggttg tattcctcgg tgccgcgctc
      541 gtcgcagttg ccggcaatca ccacgttcac ctggggatac cgctggagcc aggcggcctg
      601 acggtccagt gtctgacggg cctggtcgtc cagcgacgag cggtccgtgg cgaagaagat
      661 gcggtcgccc acggtttgca ccaggtcttc ctgggaaccc ggtgccgcct gcccggcgcc
      721 atagccattg gcgccataag cgccggcccc gggagcaccg gccccctgct gcccataggc
      781 gccgcccgcc ccgttgctgg cgccctcatc ggacgcgcag ccggccagca ggcccaccac
      841 ggcaaaggca cagatcactt ggcgaaacat gcggtatcct tgtctgctgt tcgaccggtg
      901 ccgcggtcgc gtgggatcgc agcgccgcct gcatcctcct ttggccgtga gtcggcaacc
      961 ggcaaatgcg tggcgcaaca atccggccgg ggacgggctg gacccacccc tgccgcgctt
     1021 ccactatgcc ggggccgcaa tcgaaggagc gccgcatggc ccgcgtgccc tatctggacg
     1081 tcgccgacct gccgccggag gagcaggacc tgctgaaacg ccccatcgcc ctgcacaagg
     1141 cactggtcaa ctcgcccaag ggcgcacgcg ccttcgccgc gctgggccag ttcatccgct
     1201 acggcagcaa gctggacccg cggctgcgcg aactggccat cctccaggtc ggctggctgg
     1261 cgcgctcgcc gtatgaatgg tcgcaccaca tcaagatcgg ccacgatttc ggggtaacgg
     1321 acgccgatat ccgcgctttg atcgaggaat cggcgggccg tgcaacccag ttggacgcgc
     1381 tgacccgcac cgtcctgcgc gccgcgcggg aaatgaccgg ccagggtgcg atgagcgatg
     1441 ccagctttgc cgccttgcag gcggccctgg gccatgagca ggtggttgac ctgacgctga
     1501 ccatcggctt ctacaatgcg gtcgtgcggg tgctggcaac gctgcagatc gacgtggagc
     1561 ccgaatacgc gccctatctg cagcaatttc cgttgccgca atgatgtcgc gctggttcaa
     1621 ctccttgcga gcggcgcctg ccgaattcgc ttgatgtcag agcatcttgt agcaatgcgg
     1681 ccatgacagc acggggggcg ttgggggagg gcggtgtaca gcgtggtgcc ggatcggccg
     1741 gcccggtgac gttcgattcc gaggccgtgc tggatgccat gccggacccg ttctatgcgg
     1801 tcgggcccga ttggcggctg ttgtatgtcc atcgcgccgc ggaatgcgtc tggaatgtcc
     1861 gccgcgccga ggtgattggc cgcggcctgt tggaagtgtt tcccgcgctg gctggctccg
     1921 agtcgcaccg cgccatgcag caggccatgg cgaccggcga gccgcgccgc ctggaagctg
     1981 tgtccagcgt gcttggcctc cccttcgaag tggacctgaa ccccatggcg tggggtcttg
     2041 cgatccatgt ccacgacctg accgcgcgca agaggatgga aggccagctg cgggagcgcg
     2101 gcgaactgct gaccctggcc gagcagtccg ccggtgtcgg cgtgtgggag atcgacattg
     2161 cgagcgacac cgttcgcggc accccgcaat acttccgcat catggggctg gaaccgaccg
     2221 ccgatccggt acccatggcg cgcatccgcg cgctgcgcat gccggaagat catgacgcat
//
LOCUS       DASSZH010000478         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_522587, whole genome shotgun sequence.
ACCESSION   DASSZH010000478 DASSZH010000000
VERSION     DASSZH010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522587"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..128)
                     /locus_tag="VFN42_10460"
     CDS             complement(<1..128)
                     /locus_tag="VFN42_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011253300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET8997075.1"
                     /translation="MTKRVESKYKINRRLGVNLWGRSKSPINKREYGPGQHGQRRK"
     gene            367..1116
                     /locus_tag="VFN42_10465"
     CDS             367..1116
                     /locus_tag="VFN42_10465"
                     /inference="COORDINATES: protein motif:HMM:NF005451.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET8997076.1"
                     /translation="MDIAANPTTTNDAEWQARIDLAAVYRLLAHYGWGDVIFNHAAMR
                     MPSNPRHFLIKRHELLYTEVTASNLVEVSMDADLDERAGVNRPGFTLHSGVLRARPDV
                     NCSVHVHPEEGVALSALKHGLRPLSQNAMRFYNRTGYHDYEGLTDSFAERERITAALG
                     HNRALIMRHHGITTVGVTAREAFSLMKEFVRAARIQLQIEATGADVLEIPAEVCEHVA
                     AQYEQHDRGRASAEWPAYLRMLDRIDPGYRE"
     gene            1483..2013
                     /locus_tag="VFN42_10470"
     CDS             1483..2013
                     /locus_tag="VFN42_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8997077.1"
                     /translation="MNATTLRTARLLMTPVGGADLADLQAIKADPRVFAVMLGGVRSP
                     ARTAEELAQDVGAWGAHGYGIWAVREIAGGKFVGITGLEHRPDGRGVALRFALWPEAQ
                     GRGLAREAATAALRFGHDQAGLARIIAVARANNFGSRTVLGSIGMRECGSFVRDDWTM
                     LVYESVRDPQQPALSP"
     gene            complement(2002..>2214)
                     /locus_tag="VFN42_10475"
     CDS             complement(2002..>2214)
                     /locus_tag="VFN42_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997078.1"
                     /translation="TGASDAVPETIEASLQLSEALLVDLGIPMGPVLVSIHEKRAELQ
                     EGIKSMAPQAEVRSYGRYRLRDDLRR"
BASE COUNT          356 a          784 c          715 g          359 t
ORIGIN      
        1 ttcttgcgcc gctggccgtg ctggccgggg ccatattcgc gcttgttgat gggggacttg
       61 gaccggcccc agaggttcac ccccaggcga cggttgatct tgtacttgct ctcgacgcgc
      121 ttggtcatgg ttgcgcgctg ccctttctat cctgttgttg caccggtagg ccccgcggca
      181 atgccgcggg acgggcgcag gccccgaaag cctgtccacg ttcccggcca tgggtgagcc
      241 cggcgcggag gcgccgaacc cgggcggccg tataggggcc gggcggcggc aagtcaaacc
      301 acgccgattg ccgcccgccg ccgcgccccg ctagtctcgg cacaaaccca gggaggacct
      361 cagccaatgg acatcgcggc gaaccccacc acgaccaacg atgccgaatg gcaggcccgt
      421 atcgacctcg ccgcggtcta tcgcctgctg gcgcattacg gctggggcga cgtgatcttc
      481 aaccacgccg ctatgcgcat gccctccaac ccgcgccact tcctgatcaa acggcacgag
      541 ctgctatata ccgaggtcac cgcatccaat ctggtcgaag tcagcatgga tgccgacctg
      601 gacgagcgcg ccggcgtcaa ccgacccggc ttcaccctgc acagcggcgt gctgcgcgcg
      661 cggcccgacg tgaactgctc cgtgcacgtg catcccgagg agggcgtggc gctgtccgcc
      721 ctgaaacacg gcctgcgccc gctgtcgcag aacgccatgc gtttctacaa ccgcaccggc
      781 taccacgact acgaaggcct caccgacagt ttcgccgagc gcgaacgcat taccgccgcg
      841 ctgggacaca accgcgcgtt gatcatgcgc catcacggga tcaccaccgt cggcgtcacc
      901 gcgcgcgagg ccttcagcct gatgaaggaa ttcgtacgcg ccgcccggat tcagctacag
      961 atcgaggcga ccggcgccga cgtgctggaa attccggcgg aggtctgcga gcacgtcgcg
     1021 gcgcaatacg aacaacatga cagggggcgg gcatcggccg aatggcccgc gtaccttcgc
     1081 atgctcgacc gcatcgatcc gggctaccgg gaatgagttg aagtgtgcat tcggttcatc
     1141 gcatcgcttt ttgtgcgcac ctgcgcggtt cgttctgccg caagatgtca acacggagca
     1201 cgcgaagaac cacgaaaccg cacggagcgg ctttcctgac atgatgcatc gctgagcgcc
     1261 gccgactcat ccatatcgac aatgcgcctt gtgggtcccc ggctgcggca tggcggcatg
     1321 gcttcgtggt cctccgtggc tcttcgtgtg ctccgtgtcg aaagctgaca gcagaaccaa
     1381 ccccaccagt cctgcggccc gagccgccag gccggggcta ccgcaatcgc ccggttcggc
     1441 gtagcgaacg cctccaagct gcgccatgta tcgccacgcc cgatgaacgc caccaccctg
     1501 cgcaccgccc gcctgctgat gacgccggtc ggcggcgccg atcttgccga tctccaggcc
     1561 atcaaggccg atccgcgcgt gttcgccgtc atgctcggcg gcgtccgctc gccggcccgc
     1621 accgcggagg aactggcaca ggacgtgggg gcctggggtg cgcacggcta cggcatctgg
     1681 gcggtgcggg agatcgccgg cggcaaattc gtcggcatca ccgggctgga gcaccggccc
     1741 gacggccgcg gggtcgcgct gcgctttgcc ctctggccgg aggcgcaagg ccgcggcttg
     1801 gcgcgggagg ccgcaacggc cgcgctgcgg ttcggccacg accaggccgg gctggcccgc
     1861 atcatcgccg tggcccgcgc caacaatttc ggctcgcgca ccgttctggg cagcatcggc
     1921 atgcgcgaat gcggcagttt cgtgcgggac gactggacca tgctggtgta cgagagcgtg
     1981 cgggatcccc agcagcccgc gctatcgccg tagatcgtcg cgcagccggt agcgtccgta
     2041 gctgcgcacc tcggcctgcg gcgccatcga cttgattccc tcctgcagct cggcgcgttt
     2101 ctcgtggatc gacaccagca ccggccccat cggaatgccc agatcgacca gcagcgcttc
     2161 ggatagttgc aggctggcct cgatcgtttc gggcaccgcg tcggaggcgc cggt
//
LOCUS       DASSZH010000479         8091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_165060, whole genome shotgun sequence.
ACCESSION   DASSZH010000479 DASSZH010000000
VERSION     DASSZH010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8091
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165060"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1092)
                     /locus_tag="VFN42_10480"
     CDS             complement(139..1092)
                     /locus_tag="VFN42_10480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET8997079.1"
                     /translation="MQIGFNLPISGPMAQPATMARMAREGEGLGFDYLTLTDHLVVPD
                     MRTPGYPYSESGEFYQPDPGHRHEQLTAAAWIAAQTQQIRLVLAVMVVPHRPAVLAAK
                     MLATVDVLSGGRLVVGIGAGWLKAEFDAAVTTPFAERGAVTDAYLDAFRMLWTEPHPR
                     IDGRYVTMQGLLFEPKPVQKPYPPIWVGGESGPSMRRAARIGDAWYPIGSNNKHLLDT
                     LPRLEAGIARFRRMVAEAGRDPARPGVVYRVKRFAFDAPPASDGNRRLFSGTIDQVVE
                     DVHALRALGVTGLDFDFEQTEADKALAAMRRFSDTVLPRLR"
     gene            1181..2113
                     /locus_tag="VFN42_10485"
     CDS             1181..2113
                     /locus_tag="VFN42_10485"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET8997080.1"
                     /translation="MRYVRTTMRTLVADLLGLAIGFAIGGVALVHFLPRLEAHYQPAP
                     PAVVAQAAPPLRPPAPAPLPRVVLPIFPQRQPQAAPEPPPLPHVVEPDVPSRIAGLAG
                     TGFFIGDDGTLLTAAHVVKDCSRTQVVSRFVPLSPANILATDTRDDLALLQTGDVRVP
                     AILPLGQPAGAHGKLFVLGYPSTAGEKVPEETWATLENARLPGTVGSYADPGFLVWMQ
                     ASAVRHGYSGGPIVDPRDGDVVGLVKATVDPAHLRQIRGMPGSGVAIGPGSARLIAFV
                     QRHEPWLDVTRASVEGDDPLDLARKATVHVFCWR"
     gene            complement(2121..2582)
                     /locus_tag="VFN42_10490"
     CDS             complement(2121..2582)
                     /locus_tag="VFN42_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HET8997081.1"
                     /translation="MANGPQPGSGGAKASEGAEYPDFTKLFADMKMPAMPDMEAFLAA
                     SRRNMEAFTAANKVAVEGAQAIARRHMEIMQQSMTELTEAMKALATMEGPQEKAARQA
                     EMLKQAYQRAVAHMNEMRDLIQHSNAEALALLNKRFAEAMDEVKTLTAKAK"
     gene            complement(2669..3322)
                     /locus_tag="VFN42_10495"
     CDS             complement(2669..3322)
                     /locus_tag="VFN42_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET8997082.1"
                     /translation="MSDTEFDAALIASVFRMAAERGWAQVSVAAAARAAALPLHEARA
                     RFPSKPTVLVRFGRMADQAALMDTPAEGPVRDRLFDLLMRRFDALQAHRGGILALLRA
                     LPADPPTALLLALTTRRSMRWMLEAVDVSTRGLRGELRVRGLMAVWLWTLRSWEQDET
                     EDLSTTMAALDATLRRAETAAGWLGGGIRRPDGDASAEAALTSDTPPDGMAAPPGTV"
     gene            complement(3421..3585)
                     /locus_tag="VFN42_10500"
     CDS             complement(3421..3585)
                     /locus_tag="VFN42_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997083.1"
                     /translation="MTSAFRISGSVLLLERPLPGAVLHYPGMFTPLRGPHGGLLAERL
                     PAASARRDRG"
     gene            complement(3773..4105)
                     /locus_tag="VFN42_10505"
     CDS             complement(3773..4105)
                     /locus_tag="VFN42_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997084.1"
                     /translation="MKHVLMTGMALALLTAAAQARPPKGADMSLAPWFRSLRQPGTGM
                     SCCSIADCRRTDFRIKGDRYEALVGGKWQAVPPGTVLDRTDNPTGQAIVCYTPYLGIM
                     CFIKGPET"
     gene            complement(4244..4675)
                     /locus_tag="VFN42_10510"
     CDS             complement(4244..4675)
                     /locus_tag="VFN42_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET8997085.1"
                     /translation="MSDREYPVRPIVGIGVVVFKDDSVLLVRRGKPPNIGSWTLPGGG
                     QEVGETAEEAARRELLEETGVVVGPLYFAAHVDNIRHDAAGRVQFHYTILDFAARWQS
                     GTPVAGSDVTEAVWAELDTLEKYQLWTEAYRVIALARRLVG"
     gene            complement(4672..5757)
                     /locus_tag="VFN42_10515"
     CDS             complement(4672..5757)
                     /locus_tag="VFN42_10515"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HET8997086.1"
                     /translation="MTPRTLLALVTVGVVALAGGWYFGLATTPQEQRSVDTGKPMFPG
                     LINRLDKTAKIEIIHQGKPLVIQRNGDSWGLSDRNLYPVQETKLRGMLTALTELRLVE
                     PRTSDPAQYSILGVEDPSAKDTTGTSDLLRLIDASGKPIAAVIVGHRRIRTQGSVPDQ
                     VYVRRPGARQSWLAEGNLQVDADPQVWLDRDIMNIDHAQVVRVVSTRGDSQVELARRK
                     DKLVVQSPADHPKLDAYKLEDVARALELLTFEDVHKADGTPGTPIGHSVFTTSDGMTV
                     TVTIYPPADAPPPKLDGQRHILVQFAVAGQGAEAKQAAQLEKTVAGWTYQLGAWKEQS
                     LLPSIDTLRAPVPSTAAPHAPAAAANK"
     gene            complement(5807..7720)
                     /locus_tag="VFN42_10520"
     CDS             complement(5807..7720)
                     /locus_tag="VFN42_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HET8997087.1"
                     /translation="MRGRLFTSIIGVLAVAALVVGINLFADARLADVRVDLTQNRLYT
                     LSPGTRKILADLKQPITLSLFYSRRLGATAATFGDYADRVREMLQEYAAAAHGKIRLK
                     FYNPEPFSQTEDQALADGLQGVPVDQDGTKVYFGLSGSNLLDDHRTIPFFQPDRARLL
                     EYDLTKLVYELSNPKRPVVGVMSSLPLDGNARLMMMTQGREGAQPYASTLLIQQTNQM
                     KTIPTDAQVIPKDVQVLLVAEAQHLSQNTLYAIDQFVMRGGRLMVMVDPWSEALAAEP
                     TQTGMPPSDTHSDLRKLFHAWGIEFNPNQVVGNLDSAWRVRAGADPTSGATNYVAWFN
                     IGKDGINHTDPATADLQQVSVASAGFISKAPHAGIQFTPLLTATGRDGVIPVSAVKTP
                     EPAKILASFKPDGKPRVIAARIHGELKSAFSGPPALAKGQQRPADFPAYIAHTKAAAD
                     LVVVADSDILADRFWVRINDFFGQKTAVPFSDDGPFVANLIDTLAGGEALISLRSRGD
                     NDHPFTLVNAMQARAEAKFRQTQQALQQHLTKVEQQLSTLRDGKTGGPDAASMQALIT
                     PQQQQAIDAARKDIVDTREQLRAVQRDLNHDIGRLEAELRIFNIVLVPAVLTLLALGL
                     GLVRRQRRARARA"
     gene            complement(7720..>8091)
                     /locus_tag="VFN42_10525"
     CDS             complement(7720..>8091)
                     /locus_tag="VFN42_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8997088.1"
                     /translation="VNILGSPDNGVIASGYVGALLVAGAFLSVGSALSALTRNQVIAF
                     VLGVAVCFVFAVTSYPVVSDFLTRNSPALAAIARRVTVISRFDDFTRGLVSARDLIFF
                     ASFMGFWLFLNTVLIEHRKAD"
BASE COUNT         1260 a         2726 c         2765 g         1340 t
ORIGIN      
        1 ggatgggggg aggggtcgtc cgtgagttcc cggggaccgg tcgcacgacc cctccccgca
       61 accttggtcc gctccgcggc ccaagcccgc aaggggaggg ggagcaatca caccacacca
      121 caccacacca cttcaccctc accgcagcct tggcagcacc gtgtcgctga accggcgcat
      181 ggcggcgagg gccttgtccg cttccgtctg ctcgaagtcg aagtccagtc cggtgacgcc
      241 gagcgcgcgt agcgcgtgca cgtcttccac gacctggtcg atggtaccgg agaacagccg
      301 gcgattgccg tccgacgcgg ggggcgcatc gaaggcgaac cgcttcaccc ggtacaccac
      361 gcccggcctg gccggatcgc ggccggcctc cgccaccatg cggcggaacc gcgcgatgcc
      421 agcttccagc cgcggcagcg tgtccagcag gtgcttgttg ttgctgccga tcgggtacca
      481 cgcatcgccg atccgcgccg cccggcgcat cgacgggccg ctctcgccgc ccacccagat
      541 cggcggatag ggcttctgca ccggcttcgg ttcgaacagc agcccctgca tcgtcacata
      601 gcgtccgtcg atgcgcgggt gcggctcggt ccacaacatg cggaacgcgt cgagatacgc
      661 gtcggtgacc gcgccgcgct cggcgaatgg cgtcgtcacc gcggcgtcga actcggcctt
      721 gagccagccg gcgccgatcc ccaccaccag ccgtccgccg gacagcacat cgaccgtggc
      781 gagcatcttc gccgccagca cggcgggccg gtgcggcacc accatcaccg ccagcaccag
      841 gcggatctgc tgcgtctgcg ccgcgatcca cgcggcggcg gtgagctgct cgtgccggtg
      901 gcctggatcg ggctgataga attcaccgga ctccgaatag ggataaccgg gcgtgcgcat
      961 gtccggcacc accaggtggt cggtcagcgt caggtaatcg aaaccgaggc cttcgccttc
     1021 gcgtgccatg cgggccatgg tggccggctg tgccatcggg ccgctgatgg gcaggttgaa
     1081 gccgatctgc attgggtttc ctccgccgga ttatgacgga cagcctagcg ggggtgctgc
     1141 cggctgccca ttcgcgccga caaccagcct gcaaccactg gtgcgctacg tccggaccac
     1201 catgcgaacc ctggtggcgg atctgctggg tctggcgatc ggcttcgcca ttggcggcgt
     1261 cgctctggtg catttcctgc cgcggctcga agcgcattac cagccggcgc cgccggccgt
     1321 ggtggcccag gctgcgccgc cgctgcgccc gccggctccg gccccattgc cgcgcgtcgt
     1381 gctgcccatc tttccgcagc gccagccgca ggcggcaccc gagccgccgc cgctgccgca
     1441 tgtcgtggaa ccggatgtgc catcccggat tgccggtctg gccggcaccg gatttttcat
     1501 cggcgatgac ggaacgctgc tgaccgcggc gcatgtggtg aaggattgct cgcgcaccca
     1561 ggtggtgtcg cgtttcgtcc cgctgagccc ggccaacatc ctggcgaccg ataccaggga
     1621 cgacctggcg ctgctgcaga ccggcgacgt gcgggtgccg gcgatcctgc cgctgggcca
     1681 gcccgccggc gcgcacggga agctgttcgt cctcggctat ccgtcaacgg ccggagagaa
     1741 agtgccggag gagacctggg cgacgctgga gaacgcccgg ttgcccggca cggtcgggtc
     1801 ctatgccgat cccggcttcc tggtgtggat gcaggccagt gcggtgcggc acggctacag
     1861 cggcgggccg atcgtcgatc cgcgcgacgg cgacgtggtg gggctggtca aggccacggt
     1921 ggacccggcg catttgcgcc agatccgcgg gatgcccggc agcggcgtgg ccatcgggcc
     1981 cggttcggcg cggctgatcg ccttcgtgca gcggcacgag ccttggctgg acgtgacacg
     2041 ggcctcggtg gagggcgacg acccgctcga cctcgcgcgc aaggccaccg tgcacgtctt
     2101 ctgctggcgc taggcgggcg ctacttcgcc ttcgccgtca gcgtcttcac ctcatccatt
     2161 gcctcggcga agcgcttgtt caacaaggcg agcgcttcgg cgttcgaatg ctggatcagg
     2221 tcgcgcatct cattcatgtg agcgaccgcg cgctgatagg cctgcttcag catctccgcc
     2281 tggcgtgccg ccttctcctg cgggccttcc atggtggcga gcgccttcat cgcctcggtc
     2341 agctcggtca tgctttgctg catgatctcc atgtgccggc gggcaatcgc ctgggcgcct
     2401 tccacggcca ctttgttggc ggcggtgaac gcttccatat tgcgccggct ggcggcgagg
     2461 aaagcctcca tgtccggcat cgccggcatc ttcatgtcgg cgaacagctt ggtgaagtcc
     2521 gggtattcgg cgccctcgga agctttcgcg ccgccggaac ctggctgtgg accgttggcc
     2581 atggctcgcc ctcctgtgct cgattgcaga cggcgtgaag cctacgccag ccgggacggc
     2641 ctgtcaggcg tctccatcgg gtgatccgct ataccgtgcc gggcggcgcg gccatgccgt
     2701 cgggcggcgt atcgctggtc agcgccgcct cggccgaggc atcgccatcc ggccggcgga
     2761 taccgccgcc aagccatccg gccgcggttt cggcgcggcg gagcgtggca tccagcgcgg
     2821 ccatcgtggt ggacaggtct tccgtctcgt cctgctccca gctgcgcagg gtccacagcc
     2881 agacggccat caggccgcgc acccgaagct cgccgcgcag cccgcgcgtg ctgacatcca
     2941 ccgcttccag catccagcgc atgctgcggc gcgtggtcaa ggcgagcagc agcgcggttg
     3001 gcgggtcggc gggaagagcg cgcagcagtg ccaggatgcc accgcggtgt gcctgcaggg
     3061 cgtcgaatcg gcgcatcagc aggtcgaaca gccggtcgcg gaccggacct tccgccggtg
     3121 tgtccatcag cgcggcctgg tcggccatgc ggccgaaccg caccagtacg gtgggcttgg
     3181 aggggaagcg cgcccgcgcc tcgtgcaaag gcaaggccgc cgcgcgtgcc gccgcggcga
     3241 cgctgacctg ggcccaaccg cgttcggcgg ccatgcgaaa gaccgaagcg atcagcgccg
     3301 cgtcgaattc cgtgtcgctc atggcggcaa acatgagcgc aaacgcgctg gctgtcagcc
     3361 cttaacgagc gggcagggcg cggcatacgc gatcgtggcc gcgaagggcc ggcggctgcc
     3421 ctagccgcgg tcgcgccggg cgctggcggc gggcaggcgc tccgccaaca gtccgccgtg
     3481 cgggccgcgc aacggtgtga acatgccggg ataatgcagc acggcgccgg gcaggggccg
     3541 ttccagcagc aaaacgctgc cgctgatcct gaaggcggag gtcaagccgg gttgttccgg
     3601 ctgcgctgat cgcgtgggga ccgacgccgc ggcactgccg gcgcacaaac tgacagctgt
     3661 catcaagaga attgcgcgca tggaccacct ccgcgcaaca aatgggcggc aaatgacagc
     3721 gttcccaggt gctgcacgcc gacgtgacag gcgaagcgcc tgcgccgccg gatcaggttt
     3781 ccggtccctt gatgaaacac atgatgccca gatagggcgt gtagcagacg atggcctggc
     3841 cagtgggatt gtcggtgcgg tccagcacgg tgccgggcgg cacggcctgc cacttgccgc
     3901 cgaccaatgc ctcgtagcgg tcgcccttga tgcggaagtc ggtgcgccgg caatcggcga
     3961 tcgaacagca tgacatgccg gtacccggct ggcgcaggct gcggaaccac ggcgcgagcg
     4021 acatgtcggc gcccttcggt ggacgcgcct gcgcggcagc cgtcagcagc gccagcgcca
     4081 tcccggtcat cagcacgtgt ttcattgccg aacacccagc acagtccctg cgtgcacgta
     4141 ggaagatgac agctgggaaa aacgtcgctt gaccgaaagg tgacgagccg cctgcacacg
     4201 acggatggag catgccgccg atggcgcggg agcgtaccgg cggtcagcct acgaggcggc
     4261 gtgcgagcgc gatgacgcgg taggcttcag tccacaactg gtatttttcc agcgtgtcca
     4321 gctccgccca gacggcttct gtgacatcgg agccggccac cggcgtgcca ctttgccagc
     4381 gggcggcgaa gtccaggatg gtgtagtgga actgcacacg ccctgcggca tcgtggcgga
     4441 tgttatcgac gtgggctgcg aagtacaaag ggccgacgac aacgccggtc tcctccagca
     4501 gctcgcggcg cgcggcttct tccgccgtct cgcccacctc ctgtccgccg cccggcagtg
     4561 tccagcttcc gatgttcggc ggcttgccgc gccggaccag tagaacgctg tcgtccttga
     4621 acaccacgac gccgatgccg acgatcggcc gaaccggata ctcgcgatcg ctcacttgtt
     4681 ggctgccgcc gccggcgcat gcggtgccgc cgtgctggga accggcgccc tcagggtgtc
     4741 gatgctgggc agcagcgact gctccttcca tgccccgagc tgataggtcc atcccgccac
     4801 ggtcttctcc aactgggctg cctgcttcgc ctcggcaccc tgcccggcga ccgcgaattg
     4861 caccaggata tgccgttggc cgtccagttt cggcggcggg gcgtcagccg gcgggtagat
     4921 cgtgacggtc accgtcatgc cgtccgaggt ggtgaacacc gaatgaccga tcggcgtgcc
     4981 cggcgtgcca tccgccttgt gcacgtcctc gaaggtgagc agttccagcg cgcgcgcgac
     5041 gtcctccagc ttgtacgcgt caagcttggg atggtcggcc ggcgactgga cgaccagctt
     5101 gtccttgcgg cgcgccagtt ccacctgcga gtcgccgcgc gtcgacacca cgcggaccac
     5161 ctgcgcgtgg tcgatattca tgatgtcgcg gtcaagccag acctgcgggt cggcatccac
     5221 ctgcaggttg ccctcggcga gccagctttg cctggcgccc ggacggcgca catagacctg
     5281 gtccggcacg ctgccctggg tgcggatgcg gcgatgcccg acgatgaccg cggcgatcgg
     5341 cttgccggac gcatcgatca gccgcagcag atcggacgtg ccggtggtgt ccttcgcgct
     5401 ggggtcctcg acaccgagga tcgaatactg cgccggatcc gacgtacgcg gctccaccag
     5461 gcgcaattcc gtcagcgcag tgagcatgcc gcgcagcttg gtttcctgca ccggataaag
     5521 attgcggtcg gacaggcccc agctatcgcc gttgcgctgg atcaccaagg gcttgccctg
     5581 gtggatgatc tcgatcttcg cagtcttgtc gaggcggttg atcagtccgg ggaacatcgg
     5641 cttgccggtg tccaccgagc gctgttcctg cggcgtggtg gcgagaccga aataccagcc
     5701 gccggcgaga gcgaccacgc cgaccgtgac aagcgccaga agcgtgcggg gcgtcatgcg
     5761 cgggtctccc ggctggccgc gaccatcgcc gaattgtgcc tctgcctcat gcccgtgccc
     5821 tcgcgcggcg ctggcggcgc accagcccga gtcccagtgc cagaagcgtc agaaccgccg
     5881 gtaccagcac gatgttgaag atgcgcagct ctgcctccag ccgcccgatg tcgtggttca
     5941 gatcgcgctg caccgcgcgg agctgctccc tggtgtccac aatatccttg cgcgccgcgt
     6001 cgatcgcctg ctgctgctgc ggcgtgatca gcgcctgcat gctggcggcg tcggggccgc
     6061 cggtcttgcc gtcgcgcagc gtgctgagct gctgctccac cttggtgaga tgctgctgca
     6121 gtgcctgctg ggtctggcgg aatttcgctt ccgccctggc ctgcatggcg ttcaccagcg
     6181 tgaagggatg atcgttgtcg ccgcgggagc gcaggctgat caacgcctcg ccgccggcca
     6241 gagtatcgat caggttggcg acgaagggac catcgtcgct gaacggcacc gcggtcttct
     6301 ggccgaagaa atcgttgatg cggacccaga accggtccgc caggatgtcc gaatccgcca
     6361 ccacgaccag gtcggcggct gccttggtat gcgcgatgta ggcggggaaa tcggccggcc
     6421 gctgctgtcc cttggccaag gcgggcggtc cgctgaacgc cgatttcaac tctccatgga
     6481 tgcgcgcggc gatcacgcgc ggcttgccgt ccggcttgaa gctcgcaagg atcttcgccg
     6541 gctccggtgt cttcaccgcg ctgaccggaa tgacgccgtc gcgtccggtg gcggtgagca
     6601 gcggcgtgaa ctggatgccg gcatgcggcg ccttgctgat gaaaccggcc gacgccaccg
     6661 agacctgttg cagatcggcg gtggctggat ccgtatggtt gatgccgtcc ttgccgatgt
     6721 tgaaccacgc gacgtagttg gtcgcgccgg aggtcggatc ggcaccggca cgcacgcgcc
     6781 aggcggagtc gagattgccg accacctggt tcggattgaa ttcgatgccc caggcgtgga
     6841 acagcttcct caggtcggaa tgggtgtcgc tgggcggcat gccggtctgc gtgggttcag
     6901 ccgccagggc ctcgctccac ggatcgacca tcaccatcag gcgcccgccg cgcatgacga
     6961 actggtcgat cgcatacagc gtgttctgcg acagatgctg cgcctcggcc accagaagca
     7021 cctgcacatc cttggggatc acctgggcgt cggtgggaat cgtcttcatc tggttggtct
     7081 gctggatcag cagcgtggac gcatagggct gcgccccctc gcgtccctgc gtcatcatca
     7141 tcagccgggc attgccgtcc agcggcagcg acgacatgac gcccacgacc ggacgcttcg
     7201 ggttcgacag ctcgtacacc agcttggtca ggtcatattc cagcaggcgc gcgcggtccg
     7261 gctggaagaa ggggatggtg cggtggtcgt ccagcaggtt gctgccggac aatccgaaat
     7321 acaccttggt gccgtcctga tcgaccggca cgccttgcag cccgtccgcc agcgcctggt
     7381 cctcggtctg gctgaaaggt tcgggattgt agaatttcag cctgatcttg ccgtgcgcgg
     7441 cggcggcgta ttcctgcagc atttcgcgca cccggtcggc atagtcgccg aatgtcgccg
     7501 ccgtcgcgcc gagccggcgg gaatagaaca ggctcagcgt gatcggctgc ttgagatcgg
     7561 ccaggatctt tcgcgtcccg ggcgacagcg tgtacagccg gttctgggta agatcgacac
     7621 gcacgtcggc gaggcgggca tcggcgaaca ggttgattcc gacgacgagc gcggcaacag
     7681 ccagcacgcc gatgatggag gtgaacaggc ggccgcgcat cagtcggcct tccgatgttc
     7741 gatcaggacg gtgttcagga acaaccagaa ccccatgaag ctggcgaaga agatcaggtc
     7801 gcgtgccgaa accaggccgc gggtgaagtc gtcgaagcgg ctgatcacgg tgacgcggcg
     7861 ggcaatggcg gccagggcag gggagtttcg cgtcaggaaa tcgctgacca ccgggtagga
     7921 ggtgacggcg aacacgaagc acacggcgac gcccagcacg aaggcgatga cctggttgcg
     7981 ggtgagcgcg gacagcgcgg aaccgaccga caggaaggcg ccggccacca gcagcgcacc
     8041 gacatagccg gatgcgatca ccccgttatc cggtgaaccg aggatgttca c
//
LOCUS       DASSZH010000480         4130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1170679, whole genome shotgun sequence.
ACCESSION   DASSZH010000480 DASSZH010000000
VERSION     DASSZH010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170679"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="VFN42_10530"
     CDS             <1..946
                     /locus_tag="VFN42_10530"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET8997089.1"
                     /translation="GEDTWCQLFSEPGSGSDLAGATTRAEFKGNRWVINGQKVWTTSA
                     HHADWGLLLARTNWDVPKHQGLSYFVLDIHQPGVEVRPLRQMNGHASFNQVFFTDAEV
                     PPENLVNEIGRGWAVATTTLMHERRGADGIGRWGQSSDRQGRIHDEERAENATVMEPY
                     KWYPQRAGRVDLVVERAKATGRNTDPDVRQEIAKLLTLSKAAEWTALRARAAQMQGRP
                     QGPEGSLGKLVASHVARAAARVHTLISGADAMLTGEDGAMNGVIAEILVSVPAGSIAG
                     GTDEIQRNIISERVLGMPKEPRTDNDRPFRDVPKNLVI"
     gene            992..2047
                     /locus_tag="VFN42_10535"
     CDS             992..2047
                     /locus_tag="VFN42_10535"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007001948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HET8997090.1"
                     /translation="MQHPQTWLRVLPQGLYCEPGGFFIDPLRPVDRAVITHAHSDHAR
                     PGHRAVLASPGTLALMRARLGEDRAGGAQQALHWGESLRVGEVELSLQPAGHVLGSAQ
                     VVMTYGGARAVVSGDYKRVADPTCTGFVPVACDVFVTEATFALPVFCHPPPEQEIRRL
                     LDSVALFPDRTHVVGCYALGKCQRLIALLRQAGWDRPIWLHGALASMCAVYEAQGVAL
                     GDLRPATAAAKAELVGAIVLAPPGAITDRWARRLADPVVALASGWMRVRQRAKSRGVE
                     LPLVISDHADWGELNATLDAVGAPEVWVTHGREEALIHAAEQRGIRGRALRLIGYGEE
                     DEAEDAPDPPTPAEAAQ"
     gene            complement(2041..2997)
                     /locus_tag="VFN42_10540"
     CDS             complement(2041..2997)
                     /locus_tag="VFN42_10540"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HET8997091.1"
                     /translation="MPQPLVLLWTDSTGPYQDAIAAAGLGDRLQAVAIPRGQPPSPQQ
                     LEQAEAMLAWGAPPGLLPRMPKLRWVQALTAGVESWLALPDLPPALTLTCARGTHRES
                     MPENIVGALFHLTKPYAVAEQDQKQHRWVRRVATPLNGQTLGILGLGEIGQQVARLTD
                     ALGMRVIGTKRRPEPLPHVAQVYPPDATDEVLAQADFVLLLLPATPDTENFINAERLA
                     RMKPGAWLLNFGRGHLIADADLIDAVSSKRIAGAVLDVFRQEPLPAEHPFWATEGILV
                     LPHIGGGHPTRDRIVARLFVDNLARFLDGKPLLHVVDRQAGY"
     gene            3161..3655
                     /locus_tag="VFN42_10545"
     CDS             3161..3655
                     /locus_tag="VFN42_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HET8997092.1"
                     /translation="MAVEKQAYREAMARLGAAVNVITTDGPGGRAGFTASAVCSVTDS
                     PPTLLVCANRTNDSYPAMKQNAVLCVNTLAGAHEGLSPVFAGLTEHTMEARFEGATWH
                     PLVTGAPVLDGALVAFDCRITQIVEVGTHDVFICAVEAVEVGTVHDGLIYYARGYHRV
                     VHAE"
     gene            3700..>4130
                     /locus_tag="VFN42_10550"
     CDS             3700..>4130
                     /locus_tag="VFN42_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HET8997093.1"
                     /translation="MQLLAVGRLRDGAEAALFSRYNQRLRPALNVTEIAEARGAPAEV
                     KRREAAALLAAVPAQGFVIALDLGGEEPDSVRLAERLEHWLGLGRPLCFVIGGAEGLD
                     AAVLARADHVLSLGRLTWPHMLARVMLAEQLYRARAIAAGHP"
BASE COUNT          631 a         1346 c         1514 g          639 t
ORIGIN      
        1 cggtgaggac acctggtgcc agctgttcag tgagccgggc agcggatcgg acctcgccgg
       61 cgccacgacg cgggcggagt tcaagggcaa ccgctgggtg atcaacgggc agaaggtgtg
      121 gaccacatcg gcgcaccacg ccgattgggg gctgctgctg gcgcgcacca actgggatgt
      181 gccgaagcat cagggtctgt cgtatttcgt cctcgacatc catcagccgg gtgtggaggt
      241 gcgaccgctg cggcagatga acggccatgc cagcttcaac caggtgttct tcaccgatgc
      301 cgaggtgccg ccggagaacc tggtgaacga gatcggccgt ggctgggcgg tcgccaccac
      361 cacgctgatg cacgagcggc gcggtgcgga cggcatcggc cggtggggcc agtcgtccga
      421 ccgccagggc cgcatccatg acgaggagcg cgcggagaac gccaccgtca tggagccgta
      481 caagtggtat ccgcagcgcg ccggccgcgt ggacctggtg gtggaacgcg cgaaagcaac
      541 cgggcgcaac accgacccgg acgtgcggca ggagatcgcg aagctgctga ctctgtcgaa
      601 agcggcggaa tggacagcgc tgcgtgcccg cgcggcgcag atgcagggac gcccgcaggg
      661 gccggaaggc tcgctgggca agctggtggc gagccatgtc gcacgtgccg cggcgcgggt
      721 gcacacgctg atcagcggcg ccgacgccat gctgaccggc gaggacggtg cgatgaacgg
      781 cgtcatcgcg gagatcttgg tcagcgtccc cgccgggtcg atcgccggcg gcacagacga
      841 gattcagcgc aacatcattt ccgaacgcgt gctgggcatg ccgaaggagc cgcgcaccga
      901 caacgaccgc ccgttccgcg atgtgccgaa gaacctggtc atctgaggcg ccgggcttgg
      961 catcgccggg acatcgcccg acatagggcg ggtgcagcac ccgcaaactt ggctgagagt
     1021 cctgccccag ggcctgtatt gcgagcccgg cggcttcttc atcgacccgc tgcgtccggt
     1081 cgaccgcgcg gtcatcacgc acgcccattc cgaccacgct cgtcccggcc atcgtgccgt
     1141 gctcgccagt ccgggcaccc tcgccctgat gcgcgcgcga ctcggcgagg atcgcgccgg
     1201 tggcgcacaa caagcgctcc attggggcga gtcgctacgg gtcggcgagg ttgaactgag
     1261 cctgcagccc gccggtcatg tgctgggcag cgcccaggtg gtgatgacct atggcggcgc
     1321 ccgcgctgtg gtgagtggcg actacaagcg cgttgccgac ccgacctgca ccggtttcgt
     1381 accggtcgcc tgcgacgtgt tcgtcaccga ggccactttc gccctgccgg tgttctgcca
     1441 tccgccgccg gagcaggaaa tccgccggct gctggacagc gtggcactct tccccgatcg
     1501 cacgcatgtg gtcggctgct acgcgttggg gaaatgccag cggttgattg cgctgctgcg
     1561 ccaggccggt tgggaccggc cgatctggct gcatggcgcg ctggcctcga tgtgcgcggt
     1621 gtacgaagca cagggcgtgg cgctgggcga tctgcggccg gccaccgccg ccgcgaaagc
     1681 cgagttggtg ggcgccatcg ttctggcacc gcccggtgcc atcacggatc gctgggcgcg
     1741 ccggctggcc gacccggtgg tggcgctggc ctccggctgg atgcgggtgc ggcagcgggc
     1801 gaaatcccgg ggcgtggaac tgccgctggt gatctccgac cacgccgact ggggcgaact
     1861 gaacgccacc ctggatgcgg tcggtgctcc ggaggtctgg gtgacgcatg gccgcgagga
     1921 ggcgctgatc cacgccgccg aacagcgcgg catccgcgga cgcgcgctgc ggctgatcgg
     1981 ctatggcgag gaggacgagg cggaggacgc gcccgatccg cccacgccgg cggaggccgc
     2041 tcagtagccc gcttggcggt cgacgacgtg cagcagcggc ttgccgtcca ggaagcgcgc
     2101 gagattgtcc acgaacagcc gcgcgacgat gcggtcgcgc gtcggatggc cgccgccgat
     2161 atgcggcaga accagaatgc cctcggtcgc ccagaatggg tgttccgccg gcagcggctc
     2221 ctggcggaac acgtccagca ccgcaccggc aatccgcttg ctgctgaccg cgtcgatcag
     2281 gtcggcatcc gcgatcagat gcccgcggcc gaaattcagc agccaggcac cgggcttcat
     2341 ccgggcgagg cgctcggcat tgatgaagtt ctcggtatcc ggcgtggccg ggagcagcag
     2401 caggacgaag tccgcttgcg cgaggacctc atccgtggcg tccggcggat acacctgcgc
     2461 gacgtgcggc agcggttccg ggcggcgctt ggtgccgatc acccgcattc ccagcgcatc
     2521 ggtcaaccgc gccacctgct ggccgatctc ccccaggccc agaatgccga gcgtctggcc
     2581 gttcagcggc gtggcgacgc ggcgaaccca gcggtgctgt ttctggtcct gttccgccac
     2641 cgcgtacggc ttggtcagat ggaacagcgc gcccacaatg ttctccggca tcgactcgcg
     2701 atgcgtgccg cgggcgcagg tcagcgtcag ggcagggggc aggtccggca gcgccagcca
     2761 ggattctacc ccggcggtca gcgcctgcac ccagcgcagc ttcggcatgc gcggcagcag
     2821 cccgggcggc gcgccccagg cgagcatggc ctcggcctgc tccaactgct gcgggctggg
     2881 gggttgtccg cgcggaatgg cgacggcttg cagccgatcg ccaagaccgg cggccgcgat
     2941 tgcgtcctga taggggccgg tcgaatctgt ccagagaagc acgagcggtt ggggcatggc
     3001 ggcaacattc ggtgatttgt cccagcctga agcggtaccg cctggttcgc aagcgcgtgt
     3061 gtgtcgcgcg cgcaggctgg atgcggcagg gcgcgctgct ggcctgatcg ccgccagcgg
     3121 gtcatagtgg gccggacggg atttcgggat ggagcggacc atggctgtgg agaaacaggc
     3181 gtaccgggag gcgatggcgc gcctcggtgc ggcggtgaac gtgatcacca cggacggacc
     3241 cggtgggcgt gccggcttca ccgcatcggc cgtgtgcagc gtcacggact caccgccgac
     3301 cctgctggtg tgcgccaacc gaaccaatga ttcctatccg gcgatgaaac agaacgcggt
     3361 gctgtgcgtc aatacgctgg ccggtgcaca tgagggattg tccccggtgt ttgccggact
     3421 gacggagcac acgatggaag cgcgtttcga gggcgccacc tggcatccgc tggtgaccgg
     3481 tgcgccggtg ctggacggcg cattggtggc gttcgactgc cgcatcacgc agatcgtcga
     3541 agtcggcacg catgacgtct tcatctgcgc ggtggaagct gtggaagtcg gcacggtgca
     3601 tgatgggctg atctattacg cgcgcggcta tcaccgcgtg gtgcacgccg aatgaacgcc
     3661 ggccgagcgg cctgataggc cacagggtcc gccatggcga tgcagcttct ggcggtcggg
     3721 cgcctgcggg acggcgccga agccgcattg ttcagccgct ataaccagcg gctgcgtccc
     3781 gcgctcaacg tgacggaaat cgccgaggcg cgcggtgcgc cggccgaggt gaagcggcgg
     3841 gaagccgctg ccctgctcgc cgcggtgccg gcgcagggct ttgtcatcgc gctcgacctg
     3901 ggcggcgaag agccggacag cgtccgtttg gcggagcggt tggagcattg gcttggactc
     3961 gggcgtccgc tttgcttcgt gatcggtggc gccgaaggcc tcgatgccgc cgtcctggcg
     4021 cgcgcggatc acgttctgtc gcttggcagg ctgacctggc cgcacatgct ggcacgcgtg
     4081 atgttggcgg agcagttgta ccgcgcccgc gcgattgccg cgggccatcc
//
LOCUS       DASSZH010000481         2217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_113668, whole genome shotgun sequence.
ACCESSION   DASSZH010000481 DASSZH010000000
VERSION     DASSZH010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113668"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VFN42_10555"
     CDS             complement(<1..366)
                     /locus_tag="VFN42_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997094.1"
                     /translation="MIVDMHTHAGKPRRTGPVDQAVLATMAPGGVAAAVVSAIADLPM
                     IRRNPETKRLEKVRDPDPGECLACVEDYLTGFEATGMRVAREPHEIRAGDPALVLAIE
                     GCDFLEGDLDRLDAMAARGV"
     gene            527..1333
                     /gene="map"
                     /locus_tag="VFN42_10560"
     CDS             527..1333
                     /gene="map"
                     /locus_tag="VFN42_10560"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HET8997095.1"
                     /translation="MDGPADARRITIHAPEDFAGMRVAGRLAAETLDMITEHVRPGVS
                     TAALDRLCHEFITAHGAVPAPLNYRGYPKSICTSINHVVCHGIPGERKLENGDVLNID
                     VTVILDGWHGDSSRMYTAGTPSTRARNLIDVTYEAMLRGVDAVRPGATLGDVGHAIQS
                     FVEGHRFSVVRDFCGHGIGRRFHEPPNVLHFGRPGEGPVLQPGMFFTIEPMVNAGRPE
                     VKVLDDGWTAVTRDRSLSAQFEHMIGVTSDGYEIFTLSPAGLDKPPYRVG"
     gene            complement(1387..1872)
                     /locus_tag="VFN42_10565"
     CDS             complement(1387..1872)
                     /locus_tag="VFN42_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997096.1"
                     /translation="MIHHVSIAARQPRHVAEVLAELMDGRAYPFPGGIADSFMAVSGD
                     AHGSMIEVYPEDVVLEPGAGESQVVAGHVDAPRHVPFHLLLSVPVDRATVQRIGDREG
                     WRTRLFGRGAPGQPPVFHVIEFWIENRLMVEVATPDMVAEYTQTIQPAKLAAMFPSVA
                     A"
     gene            complement(1944..>2217)
                     /locus_tag="VFN42_10570"
     CDS             complement(1944..>2217)
                     /locus_tag="VFN42_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET8997097.1"
                     /translation="GARGIVSGGFAPGFAPPGDFLALKEAAAQGVMVVQATRAGSGRT
                     FLGTRLREAGFLIADNLNPQKARILLAMALSVTQDKEEIARIFRSY"
BASE COUNT          343 a          806 c          728 g          340 t
ORIGIN      
        1 gacgccgcgc gccgccatgg catccagccg gtcgagatcg ccttccagga agtcgcaacc
       61 ttcgatcgcc agcaccaggg ccgggtcgcc ggcgcggatc tcgtgcggct cacgggccac
      121 ccgcatgcca gtggcctcga agccggtgag ataatcctcc acgcaggcca ggcattcgcc
      181 cgggtccggg tcgcgcacct tctccaggcg cttcgtctcc gggttgcggc ggatcatcgg
      241 caggtcggcg atggcggaca ccaccgccgc ggccacgccg cccggcgcca tggtcgccag
      301 cacggcctgg tccaccggcc cggtccgccg cggcttgccc gcatgggtgt gcatatcgac
      361 gatcatcgcg cgcctgatcc gttctccccg ggttcatagc ccgagcggcc gcgccggggc
      421 caccggcaat gcccgcaact gtgccctgcc ctgccgggat cagccttgca cgctgcccct
      481 tgcttgcgca tcttgctgcg ggattacaac acggagcatt ggccagatgg acggcccagc
      541 cgacgcgaga cgcatcacga tccacgcgcc ggaagatttc gcagggatgc gcgtcgcggg
      601 ccgactggca gccgagacgc tggacatgat caccgagcac gtccgtccgg gcgtgtccac
      661 cgcggcgctc gaccggctgt gtcacgagtt catcaccgcg catggcgcgg tgccggcgcc
      721 gctgaactac cgcggctatc ccaagtcgat ctgcacctcg atcaaccacg tcgtctgcca
      781 cggcatcccc ggcgaacgga agctggagaa cggcgatgtg ctgaacatcg acgtcacggt
      841 gatcctggac ggctggcatg gcgattcttc ccgcatgtac accgcgggca cgccgtccac
      901 ccgggcgcgc aacctgatcg acgtgaccta tgaggcgatg ctgcgcgggg tggacgcggt
      961 tcggccgggc gcgaccctgg gcgatgtggg ccacgcgatc cagagcttcg tggaaggcca
     1021 ccggttcagc gtggtgcgcg acttctgcgg ccacggcatc ggccggcgct tccacgagcc
     1081 gccaaacgtg ctgcatttcg gccggcccgg cgagggcccg gtgctgcagc cgggcatgtt
     1141 cttcaccatc gagccgatgg tgaatgccgg ccggcccgag gtgaaggtgc tggacgatgg
     1201 ctggaccgcg gtcacccgcg accgcagcct gtcggcgcag ttcgagcaca tgatcggcgt
     1261 gacatccgac ggctacgaga tcttcaccct gtcacccgcc ggcctggaca agccgcccta
     1321 ccgggtgggg tagggacgtc aatagctccc cctcccgcaa ggggaggggg agcttggctg
     1381 ctggccttac gcggcgaccg acggaaacat cgccgccagc ttcgcgggct ggatcgtctg
     1441 ggtgtactcc gccaccatgt cgggcgtggc cacctccacc atcaggcggt tttcgatcca
     1501 gaactcgatc acatggaaca ccggcggctg cccaggggcg ccgcggccga acagcctggt
     1561 gcgccagccc tcacggtcgc cgatccgctg caccgtggcg cggtccaccg gcaccgacag
     1621 cagcagatgg aacggcacgt gccgcggcgc gtcgacatgg cccgcgacca cctgcgactc
     1681 gccggccccg ggctccagca caacgtcctc gggatacact tcgatcatcg agccgtgcgc
     1741 gtcgccgctg accgccatga aggaatccgc gatgccaccc gggaacgggt aggcccgccc
     1801 gtccatcagc tccgccagca cttccgcgac atgccgcggc tggcgcgcgg cgatcgagac
     1861 atggtggatc attctgttcc cctcccatag cgggacgcaa tctatcgcaa cccgcgccgg
     1921 gccgatatcc cgatttctgc cagttaatag ctgcggaaga tgcgggcgat ttcctccttg
     1981 tcctgcgtca cgctcagcgc catcgccagc aggatgcgcg ccttctgcgg gttgaggttg
     2041 tcggcgatca ggaaccctgc ctcgcgcagc cgcgtgccga ggaaggtgcg tccgctgccg
     2101 gctcttgtgg cctgcaccac catcacgccc tgcgccgccg cttccttcag cgccaggaaa
     2161 tcgccgggcg gggcgaagcc cggcgcgaac ccgccggaga cgatgccgcg cgccccg
//
LOCUS       DASSZH010000482         2077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1051493, whole genome shotgun sequence.
ACCESSION   DASSZH010000482 DASSZH010000000
VERSION     DASSZH010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051493"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VFN42_10575"
     CDS             <1..691
                     /locus_tag="VFN42_10575"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET8997098.1"
                     /translation="VASILPPPIESGPIQAEWFYQPSARLGGDAFGYQWLDETTFTAF
                     VLDVAGHGAAAALHAVSVVNVLRQRLLPGVDLRDPAAVIRNLNRMFPIERHNSLFLTI
                     WYGVYDTTSRTLAYATGGHHAAYLLAPDNASPIALATRNPSIGIVPDREMLADRVTVP
                     AGSALHLFSDGVFEIVDREGRQWNLEQLVGLLADASPPGGPQRLYTEIRAAARPGPLE
                     DDFSAVVLRFA"
     gene            706..1500
                     /locus_tag="VFN42_10580"
     CDS             706..1500
                     /locus_tag="VFN42_10580"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET8997099.1"
                     /translation="MAAQAPRILLLDAEEVVPGVTKVNLRGRLDATGVQSVDAIFARL
                     ADTQKYLVVDLSRVSFIASTGLRTLIAGARRIATRGGRMAFLQPDPSVESVLISSGTD
                     QVIPVCQTLADAVCSVCQIDADDDDMPGRALAFSLDAERTKSGIARVGAWVDELCILL
                     NLSQRTEYALRLCLEEVVTHVVAHAHPEPGVNADRIALRLIADAAQLSVTVQDRCAAY
                     NPLLEASQGAGEVPDIMGISLLRQHARDLAWSRVGRSNRLAFTIPR"
     gene            1522..1860
                     /locus_tag="VFN42_10585"
     CDS             1522..1860
                     /locus_tag="VFN42_10585"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET8997100.1"
                     /translation="MRMQAEEIAPGVVKATLIGRLDIGGSQDIDLHFTALTATCQGLI
                     VDLSQVGFVASMGLRLLIVGARTVARKGGRMVLLQPVAAVETVLVSSGTDNVVPILRD
                     LQEAIRAVSG"
     gene            1892..>2077
                     /locus_tag="VFN42_10590"
     CDS             1892..>2077
                     /locus_tag="VFN42_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997101.1"
                     /translation="MQGLAQLGTWVDQVTAALRLADAQEYALRLCLEEAVANVVMHGT
                     PATGAGADAIAVHVHSAP"
BASE COUNT          317 a          676 c          730 g          354 t
ORIGIN      
        1 cgtggcctcc attcttccgc cacccatcga gagcggtccg atccaggccg agtggtttta
       61 ccagcccagc gctcggttgg ggggcgatgc gttcggctac cagtggctgg atgaaacgac
      121 cttcaccgcc ttcgtgctgg acgtcgccgg gcacggcgcg gcggcggcgc tgcacgcggt
      181 gtcggtggtg aacgtgctac ggcagcggct gctgccgggc gtcgatctgc gcgacccggc
      241 cgcggtgatc cgcaacctca accgcatgtt ccccatcgag cggcacaact cgctgttcct
      301 gacgatctgg tacggcgtgt acgacacgac cagccgcacc ctggcttacg ccaccggcgg
      361 ccaccacgcc gcctatctgc tggccccgga caacgcctcg ccgatcgcgc ttgccacacg
      421 caatccgtcg atcggcatcg tgcccgaccg ggagatgctc gccgatcgcg tgacagtgcc
      481 ggccggcagt gcgctgcacc tgttcagtga cggcgtgttc gagatcgtcg accgggaagg
      541 gcggcagtgg aacctggagc agttggtggg actgctggcc gacgcatcgc cgccgggcgg
      601 gccgcagcgg ctgtataccg agatccgcgc cgcggcgcgt ccaggcccgc tggaggatga
      661 cttctccgcg gtcgtcctgc ggttcgcctg agggaagggc agcacatggc agcgcaagca
      721 ccccgtatcc tgctgctcga tgccgaagaa gtcgtgcccg gcgtgaccaa ggtgaacctg
      781 cgcgggcggc tggacgcgac gggtgtgcag tcggtggacg ccatcttcgc ccggctggcg
      841 gatacccaga aatacctggt ggtcgatctg tcgcgcgtca gtttcatcgc ctccaccggg
      901 ttgcgcacgc tgatcgccgg ggcgcggcgg atcgcgaccc gcggtggccg gatggcgttc
      961 ctgcagcccg atccatcggt ggagtcggtg ctgatcagca gcggcaccga tcaggtgatt
     1021 ccggtctgcc agaccctggc cgacgcggtc tgctcggtct gccagatcga tgcggatgat
     1081 gacgatatgc ccgggcgggc actggcgttt tcgctggacg ccgaacgcac caaatccggc
     1141 atcgcacgcg tgggcgcctg ggtggacgag ctgtgcatcc tgctgaatct cagccagcgc
     1201 acggaatacg cattgcggct gtgcctggag gaggtggtga cgcatgtcgt ggcgcatgcg
     1261 cacccggaac ctggcgtgaa tgccgaccgt atcgcgttgc ggctgatcgc cgatgcggca
     1321 cagctcagcg tcaccgtgca ggaccgctgt gccgcgtaca acccgctgct cgaagcatcc
     1381 cagggcgccg gcgaagtgcc cgatatcatg ggcatcagcc tgctacgtca gcatgcgcgc
     1441 gaccttgcct ggtcgcgggt cgggcgcagc aaccgcctgg ctttcacgat tccccgctag
     1501 cgcggcagaa ggcaccggcc catgcgcatg caggcggagg agatcgctcc gggtgtcgtc
     1561 aaggccacac tgatcggccg gttggatatc ggcggatcgc aggatatcga tctgcacttc
     1621 actgcattga ccgccacctg ccagggactg atcgtcgatc tgtcgcaggt tggctttgtc
     1681 gcctcgatgg ggttgcgcct gctgatcgtc ggcgcccgca cggtggcgcg gaagggcggc
     1741 cgcatggtgc tgctacagcc ggtggcggcg gtggaaaccg tgctggtcag cagcgggacg
     1801 gacaacgtcg taccgatcct gcgcgatctg caagaggcga tacgtgcggt cagcggctga
     1861 acctggcttt gcgctgcggt tggaacgcag catgcagggc cttgcgcaac tgggcacctg
     1921 ggtggaccag gtcaccgcgg cgctgcgcct ggccgatgcc caggaatacg cgttgcgcct
     1981 gtgcctggag gaagcggtcg cgaacgtggt gatgcacggc accccggcga ccggcgccgg
     2041 tgcggatgcg atcgccgtgc atgtgcacag tgcaccg
//
LOCUS       DASSZH010000483         3630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1017160, whole genome shotgun sequence.
ACCESSION   DASSZH010000483 DASSZH010000000
VERSION     DASSZH010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017160"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VFN42_10595"
     CDS             <1..681
                     /locus_tag="VFN42_10595"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003629723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(46)-N(7))-methyltransferase TrmB"
                     /protein_id="HET8997102.1"
                     /translation="PPERLYGRARGHKLRPRQERLLAVTLPRLRLRLEQAGAPLAAFP
                     TPPAQLWLEVGFGGGEHALAQIAANPESGLIACEVFENGICSLLSRLVPEVGEADAPL
                     PANLRLWPDDARVLLRALPDQSLDRLFLLFPDPWPKARHAKRRFVHPAMLPTLARVLK
                     PGAVWRVASDDPTYQAWVGEVMAAQSLFSVMPPARERPAEWPPTRYEAKALREGRQPL
                     YWTFVRRT"
     gene            855..>3630
                     /locus_tag="VFN42_10600"
     CDS             855..>3630
                     /locus_tag="VFN42_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HET8997103.1"
                     /translation="MAVSATRTRGFARPVETIETSRPVADTVKTTTCYMCACRCGIRV
                     HLKDGRIRYIDGNPDHPVNQGVLCGKGSAGIMNHYSPARLSKPLLRVGERGSGDFREI
                     EWEEALTLATQWLGDIRNSEPRKLAFFTGRDQSQSFTGWWAKQFGTPNFAAHGGFCSV
                     NMAAGGLYTVGGSFWEFGDPDWDRTKYLLMFGVAEDHDSNPIKTGIAKLKARGAKFVS
                     VNPVKTGYSAVADEWVGLRPGTDGLFVLSLVHELLLADRVDLDFLARYTNAASLVIRN
                     PGGADDGLFLRNDDGKPLAWDRASRTAMDAATPDMQPQIAGEITLPDGRRAYPVFHLL
                     AERYLDGRYAPDVVAETCGIPAATIRRIAQELADVAFDQAIELQQPWTDTFGRRHETM
                     LGRPVAMHAMRGISAHSNGFHTCRAIHLLQMLLGAIDTPGSWRYKSPFPKPMPPGPQP
                     VGKGSKPNTMLPGMALGSPRGPEDLLLNDDGSAVRIDKGFTWDAPMSAHGLMHTLIAN
                     AHAGDPYPVDTLFMYMANMAWNSSMNPGEAARMMADKDPATGAYRIPHIIYSDAYFSE
                     TVAYADLVLPDTTYLERWDCISLLDRPIGSPEGPGDSIRQPVIPPDRDVRPFQDVLLQ
                     LGVRLKLPGFVKEDGSAAYRDYADYIARHERRPGVGPLAGFRGEDGKGEGVGTPNPNQ
                     LQRYVENGCFWTYHHPPEALYYKHANAAYLADAKRLGLIDSTAQIVLQLYCEPLQKFR
                     LAAEGHGPVQPPDAQRGRIRHYFDPLPIWYPPFEQTLPEQAAYPLHAVTQRPMAMYHA
                     WHSQNAWLRQIYSHNRLYMHRGTAAALGIDDDGWVWVISRNGRLKCQVKLMEGVNPDT
                     VWTWNAIGKRRGAWNLSDDAPESERGFLLNQVISEFLAGGKQLNADPVTGQAAWYDLR
                     VRVEKADGAEV"
BASE COUNT          609 a         1195 c         1233 g          593 t
ORIGIN      
        1 ccgcctgaac gcctgtatgg ccgcgcccgc ggccacaagc tgcggccgcg gcaggagcgg
       61 ctgctcgcgg tgaccctgcc gcggctgcgg ctccgtctgg agcaggcggg cgcgccgctt
      121 gccgcctttc ctaccccgcc ggcgcagctt tggctggagg tcggcttcgg cggcggcgaa
      181 catgcgctgg cgcagatcgc ggccaacccg gaaagcggac tgatcgcctg cgaggtgttc
      241 gagaacggca tctgctccct gctgtcccgc ctggtgccgg aagtcgggga ggcggatgcg
      301 ccgctgccag ccaatctgcg gctgtggccg gacgacgccc gcgtgctgct gcgcgcgctg
      361 ccggatcaat cgctggaccg gctgttcctg ctgttccccg atccctggcc gaaggcacgc
      421 cacgccaagc ggcgcttcgt gcatcctgcc atgctgccga ccctcgcccg ggtgctgaaa
      481 cccggcgccg tgtggcgggt cgccagtgac gacccgacct atcaggcatg ggtcggcgag
      541 gtcatggccg cgcagagcct gttctcggtg atgccgccag ccagggaacg tcccgcggaa
      601 tggccgccga cccggtatga ggccaaggcg ttgcgcgagg gccgtcagcc gctgtactgg
      661 accttcgtcc gccgtacctg attgaattga tcgtctattc cgatcaatgc ccggcaagtg
      721 cgccggctgc cctggccatc cagcaacaat ctgctatgac cggctggtgg gctcgaagtc
      781 ttatctcccg gcgcacgctg cagcgcgacg ggattcccag gcacgggcgg cagagtacgc
      841 gggaggagaa ggacttggca gtttctgcaa cccggacacg cggtttcgcg cgcccggtcg
      901 agacgatcga gacctcccgg ccggtcgccg atacggtcaa aaccaccacc tgctatatgt
      961 gcgcctgccg ttgcggcatc cgcgtgcacc tgaaggatgg ccgcatccgc tacatcgacg
     1021 gcaatccgga tcacccggtg aaccagggcg tgctgtgcgg caaaggctcc gccggcatca
     1081 tgaaccacta ttcgccggct cggctcagca aaccgctgct gcgggtgggc gaacgcggca
     1141 gcggcgattt tcgcgagatc gagtgggagg aagcgcttac gctcgccacg caatggctgg
     1201 gcgacatccg caacagcgaa ccgcgcaagc tcgccttctt caccggccgc gatcagagcc
     1261 aatccttcac cggatggtgg gccaagcagt tcggcacgcc gaatttcgcc gcacatggcg
     1321 ggttctgctc ggtgaacatg gcggccggcg gcctgtatac cgtcggcggc agtttctggg
     1381 aattcggtga tcccgactgg gaccgcacga aatacctgct gatgttcggc gtcgcggagg
     1441 accacgactc caacccgatc aagaccggca tcgccaagct gaaggcgcgc ggcgccaagt
     1501 tcgtgtcggt gaacccggtg aagaccgggt attcggcggt ggccgacgaa tgggtgggac
     1561 tgcgtccggg gaccgacggg ctgttcgtgc tgtcgctggt gcacgaactg ctgctggcgg
     1621 atcgcgtcga tctggatttc ctcgcccgct acaccaatgc cgcctcgctg gtgatccgca
     1681 atcccggcgg cgcggatgac gggctgttcc tgcgcaatga cgacggcaag ccgctggcgt
     1741 gggaccgtgc ctcgcggacg gcgatggatg ccgcgacccc agacatgcag ccgcagatcg
     1801 ccggcgagat caccctgccg gacgggcgcc gcgcgtaccc ggtgtttcac ctgctggcgg
     1861 agcgctacct ggacggccgc tacgcgcccg acgtggtggc ggaaacctgc ggcattcccg
     1921 cggccaccat tcgccgcatc gcccaggaac tggccgatgt cgcgttcgac caggcgatcg
     1981 agctgcagca gccctggacg gacacattcg gccggcgcca tgagacgatg ctgggccggc
     2041 cggtggcgat gcacgcgatg cgcggcatct ccgcccattc caacgggttc cacacctgcc
     2101 gcgcgatcca cctgctgcag atgctgctgg gcgcgatcga tacccccggt tcatggcgct
     2161 acaagtcgcc ctttccgaaa ccgatgccgc cgggaccgca gccggtgggc aagggatcga
     2221 agccgaatac catgctgccc ggcatggcac tgggcagtcc gcgcggtccg gaggacctgc
     2281 tgctgaacga tgacggcagc gcggtgcgca tcgacaaagg cttcacctgg gacgcgccga
     2341 tgtcggcgca cgggctgatg cacacgctga tcgccaatgc gcatgccggc gatccgtatc
     2401 cggtggatac gctgttcatg tacatggcca acatggcatg gaactcctcg atgaatcccg
     2461 gggaggccgc gcgcatgatg gccgacaagg acccggccac gggggcctac cgcatcccgc
     2521 acatcatcta ctccgacgcg tatttctccg agacggtcgc ctatgccgac ctggtgctgc
     2581 cggacacgac ctatctggag cggtgggatt gcatatcgct gctcgaccgg ccgatcggca
     2641 gcccggaagg gccgggcgat tccattcgcc agccggtgat cccgcccgac cgcgacgtgc
     2701 ggccgttcca ggacgtattg ctgcaactcg gcgtgcggct gaaactgccg ggattcgtca
     2761 aggaagacgg cagcgcggcg taccgcgact atgccgacta catcgcgcgg cacgaacgcc
     2821 ggcccggtgt tgggccgctg gccggctttc gtggcgagga cgggaaaggg gagggtgtcg
     2881 gtacgcctaa cccgaaccag ttgcagcgtt acgtggagaa cggctgcttt tggacctatc
     2941 accatccgcc ggaagcgctg tactacaagc acgccaacgc ggcgtatctg gcggatgcga
     3001 agcggcttgg gctgatcgac agtacggcgc agatcgtgct gcaactttat tgcgagccgc
     3061 tgcagaaatt ccgtttggct gccgagggcc atgggccggt gcagccgccg gatgcccagc
     3121 gcggccgcat ccgccactac ttcgacccgc tgccgatctg gtatccgccg ttcgagcaga
     3181 cgctgccgga acaggccgcc tatccgctgc acgcggtgac gcagcggccg atggcgatgt
     3241 accacgcctg gcattcgcag aatgcctggc tgcggcagat ctacagccat aaccggctgt
     3301 acatgcatcg cggtaccgcc gcggctctgg gcatcgacga tgacggctgg gtctgggtga
     3361 tcagccgcaa cggccgactg aagtgtcagg tgaaactgat ggagggggtg aaccccgaca
     3421 ctgtgtggac ctggaatgcc atcggcaagc ggcgcggcgc ctggaacctg tcggacgatg
     3481 cgccggaatc cgagcgcggg ttcctgctga accaggtgat ctcggaattt ctggcgggcg
     3541 ggaagcagtt gaatgccgac cccgtcaccg gccaggcggc gtggtacgac ctgcgcgtac
     3601 gggtggaaaa ggcggacggt gcggaagtca
//
LOCUS       DASSZH010000484         3935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1119540, whole genome shotgun sequence.
ACCESSION   DASSZH010000484 DASSZH010000000
VERSION     DASSZH010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119540"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VFN42_10605"
     CDS             complement(<1..253)
                     /locus_tag="VFN42_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003629553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HET8997104.1"
                     /translation="MRAPFRKMHGCGNDFVVFDERAGSLGFTPARAAALANRRTGIGC
                     DQFITIEPAPPGSRADAFMRIRNPDGGEAGACGNANRCVV"
     gene            374..1642
                     /locus_tag="VFN42_10610"
     CDS             374..1642
                     /locus_tag="VFN42_10610"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET8997105.1"
                     /translation="MQAERATIRFMAGYQAIWFRSFPDLSRRAQWHIASHLCTKARDG
                     APVGELSGMVKQLFLLDDATVRERLGEFYRLGLCSIDPPDRAVSARTIILPSEDLLTR
                     FDAHLRELGQLLFSAVREYEPGLTGGPPASLDDATRRGLLRAVEACNAGCLAQLDAGF
                     AAAGISVARRLEAKRHLLSPSHWLLVQMAVERWYGVSPQTDEGEGVLADDLAAELLKL
                     LRQNFQTTRDHIGYLVQLGLLERCPGRALRVALAEGPGAALHRALGEASAELVEVARA
                     IAVPDDPLERTAMAVPPGPLNGGLRHVLTVTRPGEQERQLVLGHEPLVIGRAPSSGLM
                     LPATEVSRSHCRLELAGGQVKLTDLESTNGTFVDGQRLSGSTGLQPGAVLQVGPYRLI
                     YAADEVEEAEGTLRSGRPPSNVTRLRRGAR"
     gene            complement(1670..>3935)
                     /locus_tag="VFN42_10615"
     CDS             complement(1670..>3935)
                     /locus_tag="VFN42_10615"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET8997106.1"
                     /translation="GKGNYAADAHPAGLLQAVLVRSPYAHARIAGIDATAAAGMPGVV
                     AVFTAADLTDVAPIPGGINFPRPDGGPAPKTDRWLLARDRVRFLGEPVALVLAETRPA
                     GLEAAESVIVDYDELPLVTDPVAALRSGAPAVWDEVPDNIGFLWKRGDLQAVDDGLKA
                     AAHVARLEFSVSRVTANSMEPRGAWAELGADGRMVVHVSHQSPHQLRNGLASGPFNLK
                     PTEIRVLAEDVGGSFGMKSGVHQEVVLAAWAARRLKRPVRWIADRTEGFLTDEQAREM
                     RITAELGLDAEGRFTALKARWDVNLGAYVSGRSAWCVGNFGGIAGVYEIPAIHGEVCG
                     ILTNTVTTAAYRGAGRPEATYTIERLIDVAARELGISPYELRRRNLIPPEKMPYKTAL
                     TFTYDCGEFEGNMTTAAGLADLAGFEARRAEAKQRGKLRGIGICNCIEVAGGPFLRPA
                     KDLSTLRLAEDGSLILRSGSMSVGQGLETAFTQLVADRFGIPQDQVKWEMGDTDQLEF
                     GKGNGGSGALCIGGAAVSLAVDAVIERARHIAAELMEAAVVDVELRDGAFRIAGTDRA
                     VTLADVARAAHDPAKVPPGEEGGLVESGEFTPTAVTFPNGTHICEVEIDPDTGVTQVV
                     GYSAVEELGRVLNPLLVSGQIHGGVAQGVGQALGEQIVHDPETGQMLTASFMDYQMPR
                     AADFPDMRLATREVPTKVNPLGAKGVGEAGTVGAMAAVMNAVNDALAPLGIRHFDMPA
                     TPGRVWGAIARAET"
BASE COUNT          561 a         1392 c         1384 g          598 t
ORIGIN      
        1 gaaccacgca acggttggcg ttgccacacg cgccggcctc gccgccgtcc gggttgcgga
       61 tgcgcatgaa ggcgtccgcc ctggaccccg ggggcgccgg ctcgatggtg atgaactggt
      121 cgcaaccgat tcccgtccgc cggttcgcca gcgccgccgc ccgcgccggg gtgaacccga
      181 gcgatccggc gcgctcgtcg aacacgacga agtcgttgcc gcagccatgc atcttgcgga
      241 agggggcgcg catagcgccg gatatagagc cgccgggcgc cctgcgccat gccgcaccgc
      301 ggcgacaaag cgagacaagc ggttccagac cggtatagtc ttgttccgat cgaggaccgg
      361 gacgggggag ccggtgcagg ccgagcgtgc cacgattcgc ttcatggcgg gataccaggc
      421 gatctggttc cggagtttcc cggacctgtc gcgccgtgcg cagtggcaca tcgccagcca
      481 tctctgcacc aaggcacgtg acggcgcgcc ggtgggcgaa ctgtccggca tggtcaagca
      541 gctcttcctg ctggacgatg ccaccgtccg cgaacggctg ggcgaattct atcgcctggg
      601 cctgtgttcg atcgatcccc ccgaccgggc ggtgtccgcg cgcaccatca tcctgcccag
      661 cgaagacctg ctgacccggt tcgacgcgca tctgcgcgaa ctggggcaac tgctgttctc
      721 cgcggtgcgc gagtacgagc ccgggctgac cggcggaccg cctgcctcgt tggacgatgc
      781 gacgcggcgc ggcctgttgc gcgcggtgga ggcctgcaat gccggatgcc tggcgcagct
      841 ggacgccggc ttcgcggcgg ccgggatctc cgtcgcacgc cggctggagg ccaagcgcca
      901 cctgctgtcg ccttcccatt ggctgctggt ccagatggcg gtggagcgtt ggtacggcgt
      961 atcgccgcag accgatgagg gcgaaggcgt gctggccgac gatctggccg ccgaactgct
     1021 gaagctgctg cggcagaact tccagaccac ccgcgaccac atcggctacc tggtgcagct
     1081 tggcctgctg gagcgttgcc cgggccgggc gctgcgcgtg gccttggcgg agggtcccgg
     1141 ggccgcgctg catcgtgccc ttggcgaggc atcggccgag ctggtggaag tcgcgcgcgc
     1201 gatcgccgtg ccggacgatc cactggaacg caccgcgatg gctgtgccgc cggggccgct
     1261 caacggtggc ctgcgccatg tgctcacggt cacccgcccg ggcgagcagg agcgccagct
     1321 tgtgctgggc catgagccgc tggtgatcgg ccgggcgcca tcgagcgggc tgatgctgcc
     1381 ggcgaccgag gtgtcccgct cgcattgccg cctggaactg gccggcggcc aggtgaagct
     1441 aaccgatctg gaatccacca acggcacctt cgtggacggg cagcggctga gcggctcgac
     1501 cgggctgcag ccgggcgcag tgctgcaggt agggccatac cggttgatct atgccgcgga
     1561 tgaggtggag gaggccgaag ggaccctgcg ctccggccgc ccgcccagca acgtcacccg
     1621 cctgcgccgc ggtgcgcgat aggctgcggc gggcccccct tcgcgcgcct cacgtttcgg
     1681 cccgcgcgat cgccccccac acccggcccg gggtggccgg catgtcgaag tggcggatac
     1741 cgagcggcgc cagggcgtcg ttgaccgcgt tcatcaccgc ggccatggcg cccaccgtgc
     1801 cggcctcgcc cacgcccttc gcgccgagcg ggttgacctt ggtggggacc tcccttgtgg
     1861 cgaggcgcat gtcgggaaag tcggcggcgc gcggcatctg gtagtccatg aaggaggcgg
     1921 tcagcatctg gccggtttcc gggtcgtgca cgatctgctc gcccaaggcc tgtccgacgc
     1981 cctgcgccac gccgccatgg atctggccgg acaccagcag cgggttcagc acgcggccga
     2041 gttcctccac cgcgctgtag ccgaccacct gggtcacgcc ggtatccgga tcgatctcca
     2101 cctcgcagat atgcgtgccg ttggggaagg tgaccgcggt gggggtgaat tcccccgatt
     2161 ccaccaatcc gccctcctcg cccggcggca ccttcgccgg gtcatgcgcg gcacgcgcca
     2221 cgtccgccag ggtgaccgcc cggtcggtgc cggcgatgcg gaaggcgccg tcgcgcagct
     2281 ccacatcgac caccgccgcc tccatcagct ccgcggcgat gtgccgggcg cgttcgatca
     2341 ccgcatccac cgccaggctg accgcggcgc caccgatgca aagcgcgccg gagccgccat
     2401 tgcccttgcc gaactccagc tggtcggtgt cgcccatctc ccacttcacc tggtcctgcg
     2461 ggatgccgaa ccgatcggcg accagctggg tgaaggcggt ttccaacccc tgcccgaccg
     2521 acatggagcc ggaccgcagg atcagcgagc cgtcctccgc cagacgcagc gtggacagat
     2581 ccttggcggg gcgcaggaac ggtccgccgg ccacctcgat gcagttgcag atgccgatgc
     2641 cgcgcagctt gccgcgctgc ttcgcctccg cccgccgcgc ctcgaagccg gcgaggtcgg
     2701 cgagaccggc ggcggtggtc atgttccctt cgaactcgcc gcaatcgtag gtgaaggtga
     2761 gtgcggtctt gtacggcatc ttctccggcg ggatcaggtt gcgccggcgc agctcgtagg
     2821 ggctgatgcc caattcgcgt gccgccacgt cgatcagccg ctcgatcgtg taggtggcct
     2881 ccggccgccc agcgccgcga taggccgcgg tggtgacggt gttggtgagg atgccgcaga
     2941 cttcgccgtg gatcgccggg atttcgtaga cgccggcgat gccgccgaaa ttgccgacgc
     3001 accaggcgga gcggccggat acgtaggcac ccaggttgac gtcccagcgc gccttcagcg
     3061 cggtgaagcg cccctcggca tccagcccca gctcggcggt gatgcgcatc tcgcgcgcct
     3121 gttcgtcggt gaggaagcct tcggtgcggt cggcaatcca gcgcaccggc cgcttcaggc
     3181 gtcgcgccgc ccaggccgcc agcacgactt cctggtgcac gccggacttc atgccgaagg
     3241 agccgccgac gtcttccgcc agcacgcgga tctccgtcgg tttcagattg aaggggccgc
     3301 tggccaggcc gttgcgcaac tggtgcgggg attggtggga gacatgcacc accatgcgcc
     3361 catcggcacc cagttccgcc caggcgccgc gcggttccat ggagttggcg gtgacgcggg
     3421 agacggaaaa ctccagccgt gcaacatgcg ccgcggcttt cagcccgtca tccaccgcct
     3481 ggaggtcgcc gcgcttccac aggaagccga tattgtccgg cacctcgtcc cacaccgccg
     3541 gcgcgccgga gcgcagcgcg gcgacgggat cggtgaccag cggcaattcg tcataatcga
     3601 cgatgacgga ttccgccgct tccaggcccg cggggcgggt ttccgccagc accagcgcca
     3661 ccggttcgcc caggaagcga acgcggtccc tggccagcag ccagcgatcg gttttcggcg
     3721 ccggcccgcc atccgggcgg gggaagttga tgccgccggg gatcggcgcc acgtcggtca
     3781 gatcggcggc ggtgaacacc gccacgacgc ccggcatgcc ggccgcggcc gtggcgtcaa
     3841 tcccggcgat gcgggcatgc gcgtagggag agcggaccag cacggcctgc agcagaccgg
     3901 ccgggtgagc gtcggcggcg tagtttccct tgccc
//
LOCUS       DASSZH010000485         4015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1408984, whole genome shotgun sequence.
ACCESSION   DASSZH010000485 DASSZH010000000
VERSION     DASSZH010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408984"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2146)
                     /gene="alaS"
                     /locus_tag="VFN42_10620"
     CDS             complement(<1..2146)
                     /gene="alaS"
                     /locus_tag="VFN42_10620"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET8997107.1"
                     /translation="MASSNDIRAAFLDYFARNGHQVVESSPLVPRNDPTLMFTNAGMV
                     QFKNVFTGQERRPYSRATTAQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYF
                     KELAIPYAWELVTRDYGLPKDKLLVTVFSEDDEAASIWKKVAGLPDERIIRIPTSDNF
                     WRMGDTGPCGPCSEIFYDHGPEIPGGPPGSPDEDGDRFIEIWNLVFMQFEEGPPGTRV
                     PLPRPSIDTGMGLERIAAILQGKHDNYDIDIFRALILASAEATGQDPDGPFRASHRVV
                     ADHLRSSAFLIADGVLPSNEGRGYVLRRIMRRAMRHATMMGAREPVMYKLVPVLLRQM
                     GTAYPELGRAEGLIIETLKLEETRFKQLLERGLGLLAEETDRLGDRQPLPGAVAFRLY
                     DTYGFPLDLTQDAMREQGRAVDLAGFEAAMAEQRTRARAAWAGSGEAATETVWFEIKE
                     RTGGTEFLGYSTETAEGEILALVADGQPAMAVKAGSEVAVVLNQTPFYAESGGQVGDT
                     GRITGPDGLLITVTDTQKKLGDLFVHLGLVTAGEARVGTPVAAEVDHARRSNIRAHHS
                     ATHLLHEALRRRLGTHVAQKGSLNAPDRLRFDVSHNRPITGEDIAAVEAEVNARIREN
                     TDVVTRLMTPDAAVELGAMALFGEKYGEEVRVVMMGAPEGNKLAWSIELCGGTHVRRT
                     GDIGYFRIVNESAVAAGVRRLEAVTGAAAEAVV"
     gene            2388..2567
                     /locus_tag="VFN42_10625"
     CDS             2388..2567
                     /locus_tag="VFN42_10625"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET8997108.1"
                     /translation="MHNAVVLQDLAAPPGNRLEALRGDRPGQHSIRINDQWRICFVWT
                     EEGPEHVEIVDYHRG"
     gene            2573..2866
                     /locus_tag="VFN42_10630"
     CDS             2573..2866
                     /locus_tag="VFN42_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HET8997109.1"
                     /translation="MRDLPPVHPGEQLREEFMQPLGLTAYRLAKDLNVPVTRIQAIIA
                     ERRGITGDTALRLGRYFGTTPEFWLNMQRDFELEQAIEALGERLEREVQPRAA"
     gene            complement(2928..3251)
                     /locus_tag="VFN42_10635"
     CDS             complement(2928..3251)
                     /locus_tag="VFN42_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997110.1"
                     /translation="MDKDALTAWALANGWQMIAGHPSLTKPRSPKEAIVRLVFKATVV
                     NLEVKKPAGKWEKISGEAYAKVVADPDTGMPGGLGLTSIPGFTMLMQDNKDRLMMERL
                     SGGKT"
     gene            complement(3550..>4015)
                     /locus_tag="VFN42_10640"
     CDS             complement(3550..>4015)
                     /locus_tag="VFN42_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HET8997111.1"
                     /translation="VNIPRQRLLRDETLLEIAATAPTTPEALARARGVTRGFAEGRSG
                     ASLLQAVAEAAAVPERDLPPAPLARDGAKPSPALVALLKVLLAAKCERHHVATRLVAS
                     SDDIERLAVEDAPDIPALHGWRRVVFGEDALALKQERIALGAAGREVKLIEV"
BASE COUNT          597 a         1366 c         1372 g          680 t
ORIGIN      
        1 cgaccaccgc ttccgccgcc gcgccggtca ctgcttccag ccggcgcacg ccggcggcca
       61 ccgcgctttc attgacgatg cggaaatagc cgatgtcgcc ggtgcggcgg acatgggtgc
      121 cgccacacag ttcgatcgac caggcgagct tgttgccttc tggcgcgccc atcatcacga
      181 cgcgcacctc ctcgccatat ttctcgccga acagcgccat ggcgccgagt tccaccgcgg
      241 catccggcgt catcagccgg gtgaccacgt cggtattctc gcggatcctg gcgttcactt
      301 cggcttccac cgcggcgatg tcctcgccgg tgatcgggcg gttatggctg acgtcgaagc
      361 gcagccggtc gggggcgttc aggctgccct tctgcgcgac atgcgtgccg aggcggcggc
      421 gcagcgcttc gtgcagcaaa tgcgtggcgg agtggtgggc acgaatgttg ctgcggcggg
      481 cgtggtccac ctcagccgcc accggcgtgc cgacgcgcgc ctcgccggca gtgaccaggc
      541 cgagatgcac gaacagatcg ccgagcttct tctgggtgtc ggtgacggtg atcagcaggc
      601 cgtcggggcc ggtgatgcgg ccggtatcgc ccacctgccc gccgctttcg gcgtagaacg
      661 gggtctggtt cagcaccacc gcgacttccg agccggcctt cacggccatg gccggctggc
      721 cgtccgccac cagggcaagg atctcgccct cggccgtctc cgtgctgtag ccgaggaatt
      781 cggtgccgcc ggtgcgttcc ttgatctcga accagacggt ttcggtggcc gcttcacccg
      841 accccgccca ggcggcgcgg gcgcgggtgc gctgctccgc catggctgcc tcgaaaccgg
      901 cgagatcgac cgcgcggccc tgttcgcgca tcgcgtcctg cgtcaggtcg agcgggaagc
      961 cgtaggtatc gtagagcctg aaggccaccg cgccgggcag cggctggcgg tcgccgaggc
     1021 ggtcggtttc ctccgccagc aggcccaggc cgcgttccag caactgcttg aagcgggtct
     1081 cctccagctt gagggtctcg atgatcagcc cctctgcgcg gccgagctcc ggataggcgg
     1141 tgcccatctg gcgcagcagg accggcacca gcttgtacat caccggctcc cgcgccccca
     1201 tcatggtggc gtggcgcatc gcgcggcgca tgatccggcg cagcacatag ccgcggccct
     1261 cattggaggg cagcacgccg tcggcgatca ggaacgcgct ggagcgcaga tggtcggcca
     1321 ccacgcggtg gctggcgcgg aacggaccgt ccgggtcctg cccggtcgcc tcggcggagg
     1381 ccaggatcag cgcgcggaaa atgtcgatgt cgtagttgtc gtgcttgcct tgcaggatgg
     1441 cggcgatgcg ctccagcccc atgccggtgt cgatgctcgg gcgcggcagc ggcacgcggg
     1501 tgccgggcgg gccttcctcg aactgcatga agaccaggtt ccagatctcg atgaaccggt
     1561 cgccgtcctc gtccgggctg cccggcgggc cgccgggaat ctccgggccg tggtcgtaga
     1621 agatctcgct gcacgggccg caggggccgg tgtcgcccat gcgccagaaa ttgtcggagg
     1681 tggggatgcg gatgatccgc tcgtccggca agccggcgac cttcttccag atcgaagccg
     1741 cctcgtcgtc ctccgagaac accgtcacca gcagcttgtc cttgggcagg ccgtagtcgc
     1801 gggtgaccag ctcccaggca taggggatgg cgagctcctt gaaataatcg ccaaaactga
     1861 aattccccag catctcgaag aaggtgtggt gccgggcggt gtagccgaca ttgtccaggt
     1921 cattgtgctt gccgccggcg cgcacgcatt tctgcgccgt ggtggcgcgg ctgtacgggc
     1981 ggcgttcctg cccggtgaag acgttcttga actgcaccat gccggcgttg gtgaacatca
     2041 acgtcgggtc gttgcgcggc accagcggcg agctttccac cacctggtgc ccgttccggg
     2101 cgaagtagtc gaggaaggcg gcgcggatat cgttgctgct ggccatggcg gtcagatggg
     2161 ttcctgtgac gcgaaagcgt gtgaggtagc gcgggcaggc ccaggagtca cgccggggcg
     2221 ggcgctaccg cttatgaacg cttgacgttc agtacgctgt gcgtgtatat attccctgca
     2281 atgatccgct ccttcgctga ccgcgatgcc gaggcgctgt tccaaggccg gttcgccccg
     2341 ggatcgggcc ggacctccag cgagccgcac agcgcaagct gcggcaattg cacaacgcgg
     2401 tcgtcctgca agacctggct gcgcccccgg gcaatcggct ggaagccctg agaggcgatc
     2461 gaccagggca gcacagcatt cggatcaacg accagtggcg catctgcttc gtatggaccg
     2521 aggaaggacc tgagcatgtc gagatcgtcg attaccatcg ggggtgacca ccatgcgtga
     2581 tcttccaccc gtccatcccg gcgagcagtt gcgggaggaa ttcatgcagc cgcttggcct
     2641 gaccgcttac cggctcgcca aggacctgaa cgtgccggtg acccgtatcc aggcgatcat
     2701 cgccgagcgc cgcggtatca cgggtgatac cgcgctgcgg ttgggacggt atttcggcac
     2761 cacaccggaa ttctggctga acatgcagcg cgacttcgag ctggaacagg cgattgaagc
     2821 cttgggcgag cggttggagc gcgaagtgca gcctcgggcg gcctgatacg agcagcgttg
     2881 gctggctacg gtggggatgg tccacagtgc ccgcggcggc ggcggcccta cgtcttcccg
     2941 ccgctcagcc gctccatcat cagccggtcc ttgttgtcct gcatcagcat ggtgaagccg
     3001 ggaatgctgg tcaggccgag cccgccgggc atgccggtat ccgggtcggc caccaccttg
     3061 gcataggcct cgccggagat tttctcccac ttgccggccg gcttcttcac ctccaggttc
     3121 accaccgtcg ccttgaacac cagccggacg atcgcctcct tcggcgagcg cggcttggtc
     3181 aggctggggt ggccggcgat catctgccat ccgttggcca gagcccaggc ggtcagtgcg
     3241 tccttgtcca tacggtggct tgctccgggt aagatgtgtc ataccagcgg cccgaagccc
     3301 ggactcgttt gtgtcatgcc cgggcttggc ccgggcaccc acgccttact gtgatgcatg
     3361 cggcaaagac gtgggtagcc gggccaagcc cggccatgac acggatgggt tagaacttcg
     3421 ggccgctggt atcagggcgt acggcgcgcc gatcctgctg gcaagtggcg ccagccgagc
     3481 cggaaccaac cactcccgtt gcgggatgca catcgaggcg ctctgacggc agctccggcg
     3541 gcccgagcgc tacacctcga tcagcttcac ctcgcgcccc gccgcaccca gcgcgatgcg
     3601 ctcctgcttc agcgccagcg catcctcgcc gaacaccacg cgccgccagc cgtgcaacgc
     3661 cgggatgtcc ggggcgtcct ccaccgccag ccgctcgata tcgtcggagg acgcgaccag
     3721 ccgcgtcgcc acgtggtgcc gctcgcattt cgccgccagc agcaccttca gcagcgccac
     3781 cagtgccgga gacggtttcg cgccgtcgcg tgcaaggggg gcggggggca ggtcgcgctc
     3841 gggcaccgcg gcggcctcgg ccacggcctg cagcagcgac gccccgctgc gtccctcggc
     3901 gaagccgcgg gtcaccccgc gcgcgcgggc cagcgcctcg ggcgtggtcg gcgcggtggc
     3961 ggcgatctcc agcagcgtct cgtcgcgcag cagccgctgg cgggggatgt tcacc
//
LOCUS       DASSZH010000486         2467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1341008, whole genome shotgun sequence.
ACCESSION   DASSZH010000486 DASSZH010000000
VERSION     DASSZH010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341008"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="VFN42_10645"
     CDS             complement(<1..938)
                     /locus_tag="VFN42_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8997112.1"
                     /translation="MSQVHVIGAGLSGLSAAVSLTAAGRTVVVHEAGPAAGGRARSYF
                     DRELGLRIDNGNHLLLSGNAAAMAYIDRIGAGGTLTGPSLPVFPFLDIPSGTSWTLRP
                     NPGRLPWWVAAPGRRVPGTRLGDYLRLAAVPRITDDRSVAEALPHGALYRRLVEPLAI
                     AALNTPPNQGLARLLGVVLRETLMRGGAACRPCFPRQGLSETLVDPAIAWLRARGGEV
                     RFGHRIAGLDVEAGRVTALLAPEGRLPVDAVVLAVPPWVATGLLPGLTAPDAFEAILN
                     IHFGVPADPGEAGFIGLIGGTAEWVFVKPGHVSVTV"
     gene            complement(935..1594)
                     /locus_tag="VFN42_10650"
     CDS             complement(935..1594)
                     /locus_tag="VFN42_10650"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET8997113.1"
                     /translation="MQSTGLVIFDCDGVLVDSEVLACRIDAACLAEFGIPVTAEQVMD
                     RYVGVSLAGMLADLEARFGRRLPAELADTLRRRTLAAFDAELTAIAGVDAVLASLALP
                     RCVASSSEPARIRHSLTLTGLLQHFEPHVFSATQVANGKPAPDLFLFAAAAMGAGPES
                     CRVIEDSLAGVQAARAAGMTVLGFTGGGHCRPGHAERLRAAGADAVFADMRELPALLA
                     A"
     gene            1712..>2467
                     /locus_tag="VFN42_10655"
     CDS             1712..>2467
                     /locus_tag="VFN42_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET8997114.1"
                     /translation="MHPLTAWTRHARPGLPLRALTMTLVLAIASCTTVPHGAIARDMP
                     TSFAPLVKRVLPAVVNIAVTETVTGDDVASKLPPELRDTPLGREFRRKYGKQREQMMG
                     AGSGFVIDPKGIIVTNNHVVGHAEKIVVSLADGRQLPARVIGTDELTDLAVIKVDAAG
                     LPHVTWGDSGKVEVGDWVLVAGNPFGLGGSVTAGIVSAEGRDLGSSPFDSFLQLDAPI
                     NPGNSGGPIFNMDGDVIGVSTAIVSPSGGSVGIG"
BASE COUNT          389 a          931 c          823 g          324 t
ORIGIN      
        1 ctgaccgtca ccgagacatg ccccggcttg acgaacaccc actccgcggt gccgccgatc
       61 aggccgatga accccgcttc cccgggatcg gccggcacgc cgaaatggat gttcaggatc
      121 gcctcgaatg cgtccggcgc ggtcaggccc ggcagcaggc cggtggcgac ccagggcggc
      181 actgccagca cgaccgcatc gaccggcagc cgcccctccg gggcaagcag cgccgtcacc
      241 cgccccgcct cgacatccag gccggcgatg cgatgaccga accgcacctc gccgccccgc
      301 gcgcgcagcc aggcgatcgc gggatccacc agggtttccg atagcccttg ccgtggaaag
      361 catggccggc acgcggctcc gccgcgcatc agcgtctccc gcagcaccac gccgagcagg
      421 cgggcgaggc cttggttggg aggggtgttg agcgcggcga tggcgagcgg ctccaccagg
      481 cggcggtaca gcgcgccgtg cggcagcgcc tcggccacgc tgcggtcgtc ggtgatgcgg
      541 ggcaccgcgg ccagccgcag atagtcgccc agccgggttc ccggcacgcg ccggccgggc
      601 gccgcaaccc accacggcag ccggcccgga ttcggccgca gcgtccagct tgtgccgctg
      661 ggaatgtcca ggaaggggaa gaccggcagc gacggcccgg tcagcgtgcc gccggcgccg
      721 atcctgtcga tatacgccat cgccgccgca ttgccggaca acagcagatg gttgccgttg
      781 tcgatgcgca acccgagttc gcggtcgaaa taggatcgcg cgcgtccgcc ggctgccggc
      841 ccggcttcat gcacgaccac cgtgcgcccg gccgcggtga gactcacggc ggcagacagg
      901 ccggacaggc ccgcgccgat gacgtggacc tggctcacgc ggcgagcagc gcgggcaatt
      961 cgcgcatgtc ggcgaacacc gcatcggccc cagccgcacg caaccgctct gcatggcccg
     1021 ggcggcaatg gccgccgccg gtgaagccca gcaccgtcat ccccgccgcg cgcgctgcct
     1081 gcacaccggc caggctgtcc tcgatcaccc ggcaggactc gggaccagcc cccattgccg
     1141 ccgcggcgaa caggaacagg tccggcgccg gcttgccatt tgccacctgg gtggcgctga
     1201 acacgtgcgg ctcgaaatgc tgcagcagcc cggtcagggt cagggagtgc cggatacgcg
     1261 ccggctcgct gctggacgcc acgcagcgtg gcagcgccaa gctggccagc acggcatcca
     1321 ccccggcgat ggcggtcagc tccgcatcga aggccgcgag agtgcgccgg cgcagcgtgt
     1381 cggccagctc cgccggcaag cggcgcccga accgcgcctc cagatcagcc agcatcccgg
     1441 ccaggctgac cccgacatag cggtccatca cctgttcggc ggtgacgggg atgccgaatt
     1501 ccgccaggca cgcggcatcg atccgacagg cgagaacctc gctgtcgacc agcaccccgt
     1561 cgcagtcgaa gatcaccagg ccagtgcttt gcatacgctg gtatttcccc tgcggccgcc
     1621 ggacggacaa gaccgggcga agccttgttg catccctgca cgcatgccat gtgaaccgca
     1681 acgccgcccg cgccgggaga tcccgaccca gatgcatccc ctcaccgcct ggaccaggca
     1741 cgcccgaccc ggcctgccgc tgcgcgcgct gacgatgacg ctcgtgctgg cgatcgcctc
     1801 ctgcaccacc gtcccgcacg gcgccattgc gcgcgacatg cccaccagct tcgccccgct
     1861 ggtcaagcgg gtgctgccgg ccgtggtgaa catcgcggtc accgaaaccg tcaccggcga
     1921 cgacgtcgcc tccaagcttc cccccgaact gcgcgacacc ccgctcggcc gcgagttccg
     1981 ccgcaagtat ggcaagcagc gggagcagat gatgggcgcc ggctccggct tcgtcatcga
     2041 tccgaagggc atcatcgtca ccaacaacca cgtcgtcggc catgccgaga agatcgtggt
     2101 ctcgctggcg gacggcaggc agctcccggc ccgggtgatc ggcaccgacg agctgaccga
     2161 cctcgcggtg atcaaggtgg atgccgccgg gctgccgcac gtcacctggg gcgattccgg
     2221 caaggtggag gtcggcgact gggtccttgt cgccggcaac ccgttcggcc tgggcggctc
     2281 ggtcaccgcc ggcatcgtct cggcggaagg ccgcgacctg ggctccagcc cgttcgacag
     2341 cttcctgcag ctcgatgcgc cgattaatcc gggcaattcc ggcgggccga ttttcaacat
     2401 ggatggcgac gtgatcggcg tttccaccgc cattgtctcg ccctccggcg gctcggtcgg
     2461 catcggc
//
LOCUS       DASSZH010000487         7807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1324130, whole genome shotgun sequence.
ACCESSION   DASSZH010000487 DASSZH010000000
VERSION     DASSZH010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7807
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324130"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /gene="der"
                     /locus_tag="VFN42_10660"
     CDS             complement(<1..926)
                     /gene="der"
                     /locus_tag="VFN42_10660"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008850826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET8997115.1"
                     /translation="MAIPVVVIAGRPNVGKSTLFNRLAGRRSALVADTPGVTRDRKEA
                     EALMRGRTVRLVDTAGLEEAAPETLAGRMRSGAATAVGMADVVLFVVDARSGLTPADR
                     HFGQWLRRQGKAVLLVANKAEGRSSNSSVLDAYELGLGDPVPVSAEHGEGIADLMTEI
                     AALLPEPDAAAEPEVERPLKLAIVGRPNAGKSTLVNRLLGEERMITGPEPGLTRDAVS
                     TLLTDAEGHQVELVDTAGLRRRARIEAPLEKMSVSAAIEALKMAEVVVLCVDAVEGLH
                     DQDLQIARLIEREGRACVLALNKWDAVPDRNAT"
     gene            complement(941..2275)
                     /locus_tag="VFN42_10665"
     CDS             complement(941..2275)
                     /locus_tag="VFN42_10665"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET8997116.1"
                     /translation="MAQEIGKVARRGVLLAPLALSGCGLWNHWFGENKKPIPGKREPV
                     LTASHGLRVEANPPKVVLPPPVRNAAWPQAGGNPAHLMGHLEARAELAEAWRVGIGEG
                     GGYRQALLAQPVVAGNLVFTMDSDGVVSALQLSDGARVWRTDSKARNDRSSNIGGGLA
                     WSQDTLYVVNGLGEAVAFDPAKGTVRWRSSFGVPTRSAPTVVEGRIFFVTIEDRLVVL
                     STQDGRRLWSHEATNPLTQVLGQPAPAYANGLVVAGFGSGEIACLRADSGNVVWSDSL
                     GGVSASATLADFSSIRGRPVISGGKLFAVGMGGLTVGMDLPTGRRLWELNAASENSPW
                     VAGSWMFMISQDQELAAIDTANGSVAWVSALPRWKNEKEHSGLITWWGPTLLSDRLVV
                     TGTTGEALALSPYTGAILGRQRLSSPAAPLEPVVAQGTLLVVTNAGRLLALR"
     gene            complement(2282..2941)
                     /locus_tag="VFN42_10670"
     CDS             complement(2282..2941)
                     /locus_tag="VFN42_10670"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET8997117.1"
                     /translation="MVDIFDEVEEELRAERAQLLLKKYGGLMLAAAVLVVAGVGAWQG
                     WRWWQARHDMAVAGQYLTAMSTADAAGTDKAKQQQALAAFEHVSNTAPAGYRTLARLR
                     AAALMAQTGNVAGAAKLWDAVASDESAQPLLRQLASLLWAQHLIGSADPGVLQGRLMP
                     LTSPDNAWHALAKEQLAVLDLRLNKKDAAKTELRQVAGDVTAPQGVRGRAAALLAQLG
                     S"
     gene            complement(3097..4011)
                     /gene="argB"
                     /locus_tag="VFN42_10675"
     CDS             complement(3097..4011)
                     /gene="argB"
                     /locus_tag="VFN42_10675"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011253349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HET8997118.1"
                     /translation="MSDPAPTSPIDIAAATEQARVLAQALPFLRRYAGATVVVKYGGH
                     AMGEEHLAEQFGRDIALLKQVGMNPVVVHGGGPQINAMLKRLAIQSTFVDGLRVTDAA
                     MVEVVEMVLAGTVNKQVANLITRAGALAVGICGKDGGMIRAKKLNRTRKDPDSNIERA
                     LDLGFVGEPEHVDVRVIHALTGGGLVPVIAPVGVGPDGQTYNINADTVAAAVAGALQA
                     TRLLMLTDVAGVLDKDKRLIPDLTLAQVEERIADGTITGGMIPKVENCVEAVHQGVKG
                     AVILDGRVPHACLLELFTEAGPGTIIRR"
     gene            complement(4043..4567)
                     /locus_tag="VFN42_10680"
     CDS             complement(4043..4567)
                     /locus_tag="VFN42_10680"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HET8997119.1"
                     /translation="MARITVIQADITKLDTDAIVNAANQSLLGGGGVDGAIHRAAGPE
                     LLAECRRLGGCPTGQAKITQGYRLPARFVIHAVGPRYRDGKHGEPELLASCYRQSLEL
                     ACAHGLRSIAFPAISCGIYGYPIPDAARIAIGTVAAGLEAAPAIQQAVFACFGRDVLD
                     AFQAALAALPGATA"
     gene            complement(4579..5559)
                     /locus_tag="VFN42_10685"
     CDS             complement(4579..5559)
                     /locus_tag="VFN42_10685"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET8997120.1"
                     /translation="MLNAYTCDKIAIRRVASISEGSLRQAAWIDLVSATPDEVRLVQQ
                     ATGLELPGESDLAEIESSSRLNAQDGVLRLSMPLVAQLDKIPSLVPGGFVLTRDRLIT
                     IRFAPSRVFDGFIERERHGGTGNSSSAHLFVGILEAIVDRQADALEHARAQLDAISHR
                     IFRTRSMKLGGGKHEDRMLRSTLAGIGQLGDLISYVRDSQVTAGRIVPFVQAMAKDWL
                     PAELAPRFTALTQDIASLNDFDTHLNDKLQFLLDATMGFINIAQNNVMKVLTVASVVG
                     IPPVLIAGIYGMNFKGMPELDWHWGYAYGLIVITLSAVIPLAWFKLRDWI"
     gene            complement(5628..7127)
                     /gene="glpD"
                     /locus_tag="VFN42_10690"
     CDS             complement(5628..7127)
                     /gene="glpD"
                     /locus_tag="VFN42_10690"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HET8997121.1"
                     /translation="MPAPDTVFDLAVIGGGINGTGIARDAAGRGLSVVLLEQGDLARA
                     TSSASTKLIHGGLRYLEHYAFRLVREALTEREVLLRAAPHLIHPMRFVLPHHPGLRPW
                     WMIRTGLFLYDHLGGRRILPGTRTLDLRRDPAGAALQPRFSRAFEYSDCWADDSRLVV
                     LNARDAAERGASILVRTRCTQARVADGLWSLTPDTGTALRARVLVNAAGPWVSQILSG
                     VVHQNAPARIRMVKGSHIVVPRLFAHDRSYIFQNEDGRICFAIPYQGDFTLIGTTDED
                     YHGDPASVAATAGDERYLCEAVSGYLATPVPPDSVVWRYAGVRPLLDDGASKAQDATR
                     DYVLRLQHHEGAPLLSVFGGKLTTYRRLAEAALTQLAPLFPGLRPAWTANAPLPGGDF
                     PWDGLATLQRDLAERCPFLPEATRQRLLRAYGTLAFTLLSGVQSPDDLGRCFGAGLYQ
                     REVDWLVRTEWARTAEDVLWRRSKLGLRMTAAEIAALQGYLAEMPAVPA"
     gene            complement(7129..>7807)
                     /locus_tag="VFN42_10695"
     CDS             complement(7129..>7807)
                     /locus_tag="VFN42_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET8997122.1"
                     /translation="SVPPDATVEDAAKLMLQRAISGLMVVDAQGQLAGVVTEGDLLRR
                     DELGTQRHRPWWLRLLVSPGRQAADFTRTHGRRVRDVMTQDVVTVAAGAPLEEVVELM
                     EQHRIKRVPVTQDGRVTGVVSRADLLRALVVSSRNDTPVSNDDRSVRDAILAALENES
                     WAPTTSLNVTVADGVVDLWGTITNDQERRAICVIAENTPGVKQVHDHLVFIEPYSGTV
                     IEAPEGC"
BASE COUNT         1143 a         2852 c         2613 g         1199 t
ORIGIN      
        1 gtggcattgc ggtcgggcac cgcgtcccac ttgttcagtg ccagcacgca ggcgcggcct
       61 tcgcgctcga tcagccgcgc gatctgcagg tcctggtcgt gcagcccctc caccgcatcg
      121 acgcacagca ccaccacctc ggccatcttc aacgcttcga tcgcggcgct gaccgacatc
      181 ttctccagcg gcgcctcgat ccgcgcgcgc cggcgcagcc cggcagtatc caccagctcc
      241 acctggtgtc cctcggcgtc ggtcagcagc gttgacaccg catcgcgggt caggcccggc
      301 tccgggccgg tgatcatccg ctcctcgccc agcagccggt tcaccagggt ggattttccg
      361 gcattcggcc ggccgacgat cgccagcttc agcggccgtt ccacctccgg ctcggccgcc
      421 gcgtccggct ccggcagcag cgcggcgatc tccgtcatca ggtcggcgat gccctcgcca
      481 tgctccgccg agaccggcac cggatcgccc aggcccagtt cgtacgcgtc cagcacggac
      541 gaattgctcg accgcccctc cgccttgttc gccaccagca gcaccgcctt gccctggcgc
      601 cgcagccact ggccgaaatg ccggtcggcc ggggtcagcc cgctgcgcgc gtccaccacg
      661 aacagcacca cgtcggccat gccgaccgcg gtggccgcgc cgctgcgcat ccgccccgcc
      721 agggtttccg gcgccgcttc ctccagcccc gcggtatcca ccagccgtac agtgcgcccg
      781 cgcatcaggg cctcggcctc cttgcggtca cgcgtcaccc ccggcgtgtc ggcgaccagg
      841 gcagagcgcc ggccggccag ccggttgaac agcgtgctct taccgacgtt cggccgcccg
      901 gcgatgacga cgacaggaat tgccatgacc gggctcgccg ctaacgcagc gcgagcagcc
      961 gcccggcgtt ggtgaccacc agcagcgtgc cttgcgcgac cacgggttcc agcggtgcgg
     1021 cgggagagga cagccgctgc cgccccagaa tggccccggt ataggggctg agcgccagtg
     1081 cttcgccggt ggtgccggtc accaccagcc ggtcgctcag cagtgtcggc ccccaccagg
     1141 tgatcaggcc ggaatgctcc ttctcgttct tccaccgcgg cagcgccgag acccaggcca
     1201 cgctgccatt ggcggtgtcg atcgccgcca gttcctggtc ttgggagatc atgaacatcc
     1261 aggagccggc gacccagggg ctgttctcac tggcagcatt gagttcccac agccgccgcc
     1321 cggtgggcag gtccataccc accgtcaggc cgcccatgcc caccgcgaat agcttgccgc
     1381 cgctgatcac cggccggccg cggatggagg agaaatcggc cagcgtggcg gaggcggaca
     1441 ccccgcccag gctgtcgctc cacaccacgt tgccgctgtc ggcacgcagg caggcgatct
     1501 cgccggaacc gaagccggcc accaccaggc cgttggcata ggccggcgcc ggctggccaa
     1561 gcacctgggt gagcggattg gtcgcctcgt ggctccacag ccggcggccg tcctgggtgg
     1621 acagcaccac cagccggtcc tcgatggtga cgaagaagat gcgcccttcc accaccgtcg
     1681 gcgcggaccg ggtgggaacg ccgaagctgg accgccagcg gaccgtgcct ttggccgggt
     1741 cgaaggcgac cgcctccccc aggccgttca ccacgtaaag cgtgtcctgg gaccaggcca
     1801 ggccgccgcc gatattgctg ctgcggtcgt tcctggcctt gctgtcggtg cgccagaccc
     1861 gggcgccgtc actgagctgc agcgcggaga ctacgccgtc ggaatccatg gtgaacacca
     1921 ggttcccggc caccacgggc tgcgccagca gcgcctgccg atagccgccc ccctcgccga
     1981 tcccgacccg ccaggcctcc gccagctcgg cccgcgcttc caggtgtccc atcagatgcg
     2041 ccggattgcc gccggcctgc ggccatgcgg cgttgcgcac cggcggcggc agcacgacct
     2101 tcggcgggtt cgcctcgacc cgcagtccat gactggccgt cagcaccggc tcccgcttgc
     2161 cggggatcgg cttcttgttc tcgccgaacc agtggttcca cagcccgcag ccgctcaagg
     2221 ccagcggtgc cagcagcacg ccgcggcggg cgactttccc gatttcctgc gccatctctg
     2281 cctagctgcc cagttgtgcc agaagcgccg ccgctcggcc gcgcacgccc tgcggagcgg
     2341 tcacgtcccc cgccacctgg cgcagctcgg tcttcgccgc atccttcttg ttcagccgca
     2401 ggtcgagcac cgcaagctgc tcctttgcca gggcgtgcca ggcgttgtcg gggctggtca
     2461 gcggcatcag ccgcccctgc agcacccccg ggtccgcgct gccgatcagg tgctgcgccc
     2521 agagcaggct ggcaagctgg cgcagcagcg gctgcgccga ttcgtccgac gccaccgcgt
     2581 cccacagctt ggccgccccc gcgacgttgc cggtttgcgc catcagcgcc gcggcgcgca
     2641 ggcgcgccag cgtgcggtag ccggccggcg cggtgttgct gacatgctcg aacgcagcga
     2701 gcgcctgctg ctgctttgcc ttgtcggtgc cggccgcgtc cgcggtgctc atcgcggtca
     2761 ggtactggcc ggcgacggcc atgtcgtgcc gcgcctgcca ccagcgccag ccctgccagg
     2821 cgccgacgcc ggcgacaacc agcacggcgg cggcgagcat caggccgccg tacttcttca
     2881 gcagaagctg ggcacgttcg gcgcgaagtt cttcttcgac ctcatcaaag atatcgacca
     2941 ctgtgggctc tgctccgttc cggcctgcgg cgcggcggac catagcggcg gcgcggcgag
     3001 ccggcaaacc gccgcgaacc aaccgccgcg aaccatcccg catcctggcc ggccgccgcc
     3061 agcgaccgcc aggacgcgcc gccgccgccg cctcggctag cgccggatga tcgtccccgg
     3121 gccggcttcg gtgaacagtt ccagcaggca ggcatgcggc accctgccgt ccaggatcac
     3181 cgcgcccttc accccctggt gcaccgcctc gacgcagttc tccaccttgg ggatcatgcc
     3241 gccggtgatc gtgccgtcgg cgatgcgctc ctccacctgg gccagcgtca ggtcggggat
     3301 cagccgcttg tccttgtcca gcacgccggc aacgtcggtc agcatcagca gccgcgtggc
     3361 ctgcagcgcg ccggcgacgg cagcggccac cgtgtcggcg ttgatgttgt aggtctgccc
     3421 gtccggcccc acgccgactg gagcgatcac cggcaccagc ccgccgccgg tcagcgcatg
     3481 gatgacccgt acgtccacat gctccggctc gccgacgaag cccaggtcca gcgcgcgctc
     3541 gatgttgctg tccgggtcct tcctggtgcg gttcagcttc ttcgcccgga tcatgccgcc
     3601 gtccttgccg cagatgccca ccgccagggc accggcgcgc gtgatcaggt tggccacctg
     3661 tttgttgacg gtgccggcca gcaccatctc caccacttcg accatcgcgg cgtcggtgac
     3721 gcgcaggcca tcgacgaagg tggactggat ggccagccgt ttcagcatgg cgttgatctg
     3781 cgggccaccg ccatgcacca ccaccgggtt catgccgacc tgcttcagca gcgcgatgtc
     3841 gcggccgaac tgctccgcca gatgctcctc gcccatggcg tgaccgccgt atttcaccac
     3901 caccgtggcg ccggcatagc ggcgcaggaa cggcagggcc tgggccagca cgcgcgcctg
     3961 ctcggtggcg gcggcaatgt cgatcgggga ggtgggggct gggtcgctca tgcgcggcgt
     4021 gtacgggtgg tgggttgggg ggtcaagcgg tggctccggg caatgcggcg agcgctgcct
     4081 ggaaagcgtc cagcacgtcc cggccgaagc aggcgaacac cgcttgctgg atggcgggag
     4141 cagcctccag gccggccgcg acggtaccga ttgcgatgcg cgctgcatcc gggatcggat
     4201 agccgtagat gccgcaactg atcgccggaa atgcgatcga tcgcaggcca tgcgcgcagg
     4261 ccagctccag gctttgccgg tagcagcttg ccagcagctc cggctcgcca tgcttgccgt
     4321 cgcgatagcg cggcccgacc gcgtggatga cgaatctggc cggcagccgg tagccctggg
     4381 tgatcttcgc ctggccggtg ggacaaccgc ccagcctgcg gcattccgcc agcagctccg
     4441 ggccggcggc acgatggatc gcaccgtcca cgccaccgcc gcccaacagc gactggttgg
     4501 cggcgttgac gatcgcgtcg gtgtccagct tggtgatgtc tgcctggatg accgtgatgc
     4561 gtgccaaggg cgcctccgtc agatccagtc gcgcagcttg aaccacgcca gcgggatcac
     4621 cgccgacaaa gtgatgacga tcaggccata ggcatagccc cagtgccagt ccagttccgg
     4681 cattcccttg aaattcatcc cgtagatgcc ggcgatcagc accggcggga tgccgaccac
     4741 cgaggccacc gtcagcacct tcatcacgtt gttctgcgcg atgttgatga aacccatcgt
     4801 ggcatccagc aggaattgca gcttgtcgtt cagatgcgtg tcgaagtcgt tcagcgacgc
     4861 gatatcctgg gtcagggcgg tgaagcgcgg cgccagctcg gccggcagcc agtccttcgc
     4921 cattgcctgc acgaagggca cgatgcgccc ggcggtaacc tggctgtccc gcacatagga
     4981 gatcaggtcg ccgagctgcc cgatcccggc cagtgtgctg cgcagcatgc gatcctcatg
     5041 cttgccgccg ccgagcttca tcgaccgtgt gcggaagatg cggtgggaga tggcgtcgag
     5101 ctgcgcccgg gcatgttcca gcgcgtcggc ctggcgatcg acgatcgcct ccaggatgcc
     5161 gacgaacagg tgtgcgctgc tgctgttgcc ggtgccgccg tgccgctcgc gctcgatgaa
     5221 gccatcgaac acccggctgg gtgcgaagcg gatcgtgatc agccggtcgc gggtcagcac
     5281 gaagccgccg ggcaccaggc tggggatttt atcgagctgg gcaaccagcg gcatgctgag
     5341 ccgcagcacg ccatcctgcg cattcagccg gctggagctt tcgatttcgg cgaggtcgga
     5401 ttcgccgggc aattccagac cggtcgcctg ctgcaccagg cggacctcat ccggcgtggc
     5461 ggagacgagg tcgatccagg ccgcctgccg cagcgacccc tccgaaatcg aggcgacccg
     5521 ccgaatcgcg atcttgtcgc aggtgtaagc gttcagcatg ggcgcagcta agcaggttcg
     5581 tgcgtggtcg cacaatttcc ctgttggacc ttttcgccca ggtttgctca ggcgggcacc
     5641 gccggcatct cggccaggta gccctggagt gcggcgatct ccgccgccgt catgcgcagg
     5701 cccagcttgc tgcgccgcca caggacatcc tcggcggtgc gcgcccactc ggtgcggacc
     5761 agccagtcca cttcgcgctg atacaggccg gcgccaaagc agcggccgag gtcgtccggg
     5821 ctttgcacgc cggacagcag cgtgaaggcc agcgtgccgt aggcgcgtag caggcgttgc
     5881 cgcgtcgcct cgggcaggaa cggacagcgt tcggcgaggt cgcgttgcag cgtcgccagg
     5941 ccgtcccacg ggaaatcgcc gccgggcagc ggcgcgtttg ccgtccaggc cggccgtagg
     6001 ccgggaaaca gcggcgccag ttgcgtcagc gcggcttcgg cgagccggcg ataggtggtc
     6061 agcttgcctc cgaacaccga cagcagcggt gcgccctcat ggtgctgcag ccgcagcacg
     6121 tagtcgcgcg tggcgtcctg cgccttgctg gcgccgtcat ccagcagcgg ccgcacgccg
     6181 gcatagcgcc acaccaccga gtccggcggc accggcgtgg cgagatagcc gctgaccgct
     6241 tcgcacaggt agcgctcatc cccggcggtc gcggcgaccg aagcaggatc gccgtggtag
     6301 tcctcgtcgg tggtgccgat cagggtgaag tcgccctggt acgggatggc gaagcagatg
     6361 cggccgtctt cgttctggaa gatgtagctg cggtcgtgcg cgaacagccg cggcaccacg
     6421 atgtggctgc ccttcaccat gcggatgcgg gccggcgcgt tctgatgcac cacgccggac
     6481 aggatctgcg acacccacgg gccggcagcg ttcaccagca cccgggcgcg cagcgccgtg
     6541 ccggtatccg gcgtcagcga ccacagccca tcggcaaccc gcgcctgggt gcagcgcgtg
     6601 cggaccagga tgctggcgcc gcgctcggcg gcatcgcggg cgttcagcac caccaggcgg
     6661 gaatcgtcgg cccagcaatc ggagtattcg aaggctcggc tgaagcgcgg ctgcaacgcc
     6721 gcgccggccg gatcgcggcg caggtccagc gtgcgcgtgc cgggcaggat gcgccgcccc
     6781 cccagatggt cgtacaggaa caggccggtg cggatcatcc accacgggcg cagcccggga
     6841 tgatgcggca gcacgaagcg catcgggtgg atcagatgcg gcgccgcgcg cagcagcacc
     6901 tcgcgctcgg tcagcgcctc gcgcaccagg cggaacgcgt agtgctccag gtagcgcagc
     6961 ccgccatgga tcagcttggt ggacgccgag gacgtcgcac gggccaggtc gccctgttcc
     7021 agcagcacca ccgacagccc gcggccggcc gcatcgcgcg cgatgccggt tccattgatg
     7081 ccgccgccga tgacggcgag gtcgaacact gtatcgggcg ccggcatgtc agcagccttc
     7141 gggtgcctcg atcaccgtgc cggaatacgg ctcgatgaac accaggtggt cgtgcacctg
     7201 cttcacgcct ggggtgttct ccgcgatgac gcagatcgcc cggcgttcct ggtcgttggt
     7261 gatggtgccc cacagatcga ccacgccatc cgccacggtg acgttcagcg acgtggtcgg
     7321 cgcccaggat tcgttttcca gcgcggcgag gatggcgtcc cgcaccgagc ggtcgtcatt
     7381 gctgaccggt gtgtcgttgc ggctggacac gaccagcgcg cgcagcaggt cggcgcgcga
     7441 caccacgccg gtcaccctgc cgtcctgcgt caccggcacg cgcttgatgc ggtgctgttc
     7501 catcagctcg accacctctt ccagcggcgc gccggcggcg acggtgacca cgtcctgcgt
     7561 catcacgtca cgcacccggc gtccgtgcgt gcgggtgaag tcggcggcct ggcggccggg
     7621 ggagaccagc aggcgcagcc accacggccg gtgccgctgc gtgccgagct cgtcacgccg
     7681 cagcaggtcg ccctcggtga ccactccggc cagctggccc tgcgcatcga ccaccatcag
     7741 gccggagatc gcccgctgca gcatcagttt ggcggcatcc tccacggtgg cgtccggcgg
     7801 caccgag
//
LOCUS       DASSZH010000488         2482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1341092, whole genome shotgun sequence.
ACCESSION   DASSZH010000488 DASSZH010000000
VERSION     DASSZH010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341092"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VFN42_10700"
     CDS             <1..807
                     /locus_tag="VFN42_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HET8997123.1"
                     /translation="QALAREDWQEAAALARKAEAAQPGATWLRHERFRLAVHASNWSE
                     ALELADVDAPKAALAVAAAEAEADPSRGLKLAHAAWKQDASLTPAALTYAERLRRSGK
                     EKRAMAVIRQTWTLAPHPDLAAFILAPVADPLGRVRAARQLAEANAGHVESRLLMARA
                     ELDAGLTGEARHQAEAARDAGLNQRRLWLLLADIEEAEHGDTEAGRAAQREALRRAAS
                     ADPDPEWQCTVCRTPHRAWVPACRSCEAPGSLRWLTPETPGAYPVPALRA"
     gene            1209..2192
                     /locus_tag="VFN42_10705"
     CDS             1209..2192
                     /locus_tag="VFN42_10705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8997124.1"
                     /translation="MKILLLGKIGGITRWVEDVAEDLALAGHTVKLVPTRNPRLSKSL
                     ERLLLSPMVGAPLAQLAVRAMRRFAPDLILAIGALDQHPRELFLPLFAATGRPPLVAW
                     TGDSFDQDTAGIADLFDAVAYTDTGFLDLHRRFACRSAPHFLPLGASRARMVDAALAG
                     ARKPHLAFVAGPTPNRRAVLAATRHKVAIYGPGWQHAAELTQHDRHSHRVLAPELARI
                     HAEHLGVLNIRHGTHVINGLNQRHFAPYVQGAPVVSDAQPDIPQCFDPGTEMLVFQDV
                     EELNELQAELLRAPERARAVAIAGQRRVLAQHTYAHRVAALAALMGVRPPG"
     gene            2265..>2482
                     /locus_tag="VFN42_10710"
     CDS             2265..>2482
                     /locus_tag="VFN42_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997125.1"
                     /translation="MQLADLPTPCLVLDRVILERNIRRMAEAVGRHGVGLRPHMKTAK
                     SIDVARLALEDQPGGITVSTLAEAEYFAA"
BASE COUNT          371 a          866 c          863 g          382 t
ORIGIN      
        1 caggcgctgg cgcgggagga ttggcaggaa gcggcggcgt tggcccgcaa ggcagaagcg
       61 gcgcaacccg gcgccacctg gttacggcac gagcgcttcc gcctcgcggt gcatgcgagc
      121 aattggtcgg aagccctcga acttgcggat gtcgacgcac ccaaggcggc gctcgcggtg
      181 gcggcggcgg aagcggaagc cgatcccagc cgcggactga agctggcgca tgccgcctgg
      241 aagcaggatg ccagcctcac gcctgcggcg ctgacctatg ccgagcggct gcgccgcagc
      301 ggcaaggaga agcgcgcgat ggcggtgatc cgccagacgt ggacgctggc gccgcatccg
      361 gatctggcgg ccttcatcct ggcgccggtt gccgacccgc tggggcgggt gagagccgcg
      421 cgccaactgg ccgaggcgaa cgccggccat gtggagagcc gcctgttgat ggcgcgcgcc
      481 gaactggatg ccggcttgac cggcgaggcc cgccaccagg cggaagccgc gcgcgatgcc
      541 ggtctgaatc agcggcggct ctggctgctg ctggcggata tcgaggaagc ggagcacggc
      601 gacacggaag ccggccgggc agcccagcgc gaggcgctgc ggcgagcggc gtcggcggat
      661 ccggacccgg agtggcaatg cacggtctgc cggacgccgc atcgggcctg ggtccctgcg
      721 tgccggtcct gcgaggcccc cggcagcctg cgctggctga ctcccgagac gccgggcgcc
      781 tatccggtgc ctgcgttgcg cgcgtagatt ctgcgccatg ccgggcgccg ggggacacgc
      841 gtacgccgtc atggcctgcg aagggggctg taatccgcca aacgtcatgg ccgggccttg
      901 acccggccat ctaccctgga tgatcgggca ttcggggtgt gttgcgaccc gatcgttgcg
      961 gggtggatgg ccgggtcaaa gcccggccat gacggagtgg gattggatta cacgctcgaa
     1021 ggccaggaga agtcgtagat ggtcggcttc gcccgccacg acgctgtctc ccggccggtc
     1081 cacccaattg ggttacagcc gcgaagtccg gccatccacg cctggctggc aaagacagtt
     1141 ctccgcattt gctccgagtc actctcggca agccgtgggg ggcgtgcctt tgcctcgcct
     1201 tcgcacccat gaagatcctg ctcctcggca agatcggcgg catcacccgc tgggtcgagg
     1261 atgtggccga agacctggcc ctggccggcc atacggtgaa gctggtccct acccgcaacc
     1321 ccaggctgag caagtcgctg gagcggctgc tgctgtcccc gatggtcggc gccccgttgg
     1381 cccagcttgc ggtccgggcg atgcggcgct tcgccccgga cctgatcctg gcgatcggcg
     1441 ccctggacca gcacccacgt gagctgtttc tgccgctgtt cgctgccacg gggcgtcctc
     1501 cgctggtcgc ctggacaggc gacagcttcg accaggacac cgcagggatc gccgacctgt
     1561 tcgacgcggt ggcctatacc gacaccggtt tcctggatct gcaccgccgc tttgcctgcc
     1621 gctcggcacc gcacttcctg ccgcttggcg ccagccgcgc gcgcatggtg gacgccgcgc
     1681 tggccggcgc gcgaaagccg catctggcct tcgttgccgg cccaacaccg aaccgtcgcg
     1741 ccgtcctcgc cgccacacgg cacaaggtgg cgatctacgg tcccggctgg cagcatgcgg
     1801 cggaactgac ccagcacgac cggcacagcc accgggtgct ggcgccggaa ctggcgcgca
     1861 ttcacgccga gcatctgggg gtgctgaaca tccgccacgg cacacacgtc atcaatggcc
     1921 tgaatcagcg gcattttgcc ccttacgtgc agggcgcgcc ggtggtttcg gatgcgcagc
     1981 cggatattcc gcaatgcttc gatccgggga ccgaaatgct ggtgttccag gatgttgagg
     2041 agctgaacga actccaggcc gaactgctgc gggcgcccga gcgtgcccgc gccgttgcca
     2101 tcgccgggca gcggcgggta ctggcgcagc acacctacgc ccatcgcgtc gccgcgctgg
     2161 cggcactgat gggcgttcgt ccgccggggt aaccacccgc gcttgctttt gcccggccct
     2221 tcgccaacag agacgcaatc ccactcttgc gccgcggagc gatcatgcag ctcgccgacc
     2281 ttcccacgcc ctgcctcgtg ctcgaccgcg tcatcctgga gcgcaacatc cgccgcatgg
     2341 ccgaagctgt gggccggcac ggcgttggcc tgcgcccgca catgaaaacg gcaaagtcga
     2401 tcgacgtcgc ccgcctggct ttggaggacc agccgggcgg gatcaccgtc tccaccctgg
     2461 ctgaggccga gtatttcgcc gc
//
LOCUS       DASSZH010000489         3269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_574035, whole genome shotgun sequence.
ACCESSION   DASSZH010000489 DASSZH010000000
VERSION     DASSZH010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574035"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..1280)
                     /locus_tag="VFN42_10715"
     CDS             complement(51..1280)
                     /locus_tag="VFN42_10715"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET8997126.1"
                     /translation="MNGSGPETCLTGMKVLDLTQFEAGPSCTEALAWMGADVVKVENP
                     KVGDPGRTAGGQPGTDAFYFLQYNANKKSVTINLKDPRGVELVKQMAKSADVFCENFA
                     PGAIERLGLGPEVIRAINPAIIYCQTKGFGAGSAFEKSLAFDMIAQAAGGLMSITGEE
                     DGPPCKPGATIGDTGTGMLMAITVLGAYVRRLRTGQGEHLQLAMQDAILHYIRNSFTY
                     MERTGKPAPRAGARTVGGGNPPIGVYPCKGGGPNDYVYIYCSRANPEHWNRLLRVMGR
                     EDLIGDARYDSPDARTERRAEVDALVSEWTRQHDKHEAMRLVGAATIPAGAVMDTMEL
                     ASDPSFVQRGIRQVMQHPTVGEYTMSGWPVRFSGRTPPISAAPLLGEHNEDVLADWLG
                     LDRDAIGTLRQDAVIGG"
     gene            complement(1367..2104)
                     /locus_tag="VFN42_10720"
     CDS             complement(1367..2104)
                     /locus_tag="VFN42_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET8997127.1"
                     /translation="MHSLSAPLLFAAFSFLLAGSAKGVAGMGLPTVAMGLLGLQMPPA
                     EAASLIVFPSLATNLWQYLGGPHRLGLLRRLWPMLLAICAATWAGAGLLVNGVARDAT
                     LALGGALLAYGLFGLARLRLAVRPNHERWLSPAAGLATGVITGATGVFVLPAVPYLQA
                     LDLKKDVLVQALGLSFTVSTLSLAAGLATRGALQVTAAGESLACILPALAGMALGQRL
                     RNRIAQASFRRVFFLALLALGAALVAR"
     gene            2194..3096
                     /locus_tag="VFN42_10725"
     CDS             2194..3096
                     /locus_tag="VFN42_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET8997128.1"
                     /translation="MHFDLVDLRLFARVAETATITQAAAMSGLALASASERIRGMEAQ
                     AGVALLRRGRRGVTLTPAGHALAHHARMVLQQFDQLTGALSKYAGGLRGFVRLAANGS
                     ACSEFLPERLSLFLRDNPGIDVDLEEKPSFDIVRLVAEGFADIGVVADIVDFAGLQAF
                     PFARDRLVVVLPRAHPLAGQGDRHFRDLLGHEFIGMPAGNALQQHVSQHAAQAGRPLK
                     LRIRLNSFEAICRMVETGVGIAVLPETAARRCRRSMAITTCRLADGWALRQLHVCLRS
                     APDLPGPARRLAEHLIDRGTRPGR"
BASE COUNT          513 a         1132 c         1108 g          516 t
ORIGIN      
        1 aaccccctcc cgcaagggga gggggagttt tcagttcgcc tctccgcggc ctaccccccg
       61 atcaccgcat cctggcgcag cgtgccgatc gcgtcgcggt ccagcccgag ccagtccgcc
      121 agcacgtcct cattgtgctc gccgagcagc ggggccgcgc tgatcggcgg cgtgcggccg
      181 gagaagcgca ccggccagcc gctcatcgtg tactcgccga cggtgggatg ctgcatcacc
      241 tggcggatgc cgcgctgcac gaagctggga tcgctggcga gttccatcgt gtccatcacc
      301 gcgccggcgg gaatggtggc cgcgccgacc aggcgcatcg cttcgtgctt gtcgtgctgg
      361 cgcgtccact ccgacaccag cgcgtccact tccgcgcgcc gctcggtgcg cgcatccggc
      421 gagtcgtagc gcgcatcgcc gatcaggtcc tcgcggccca tcacccgcag cagccggttc
      481 caatgttcgg gattggcgcg gctgcaatag atgtagacgt agtcattggg tccgccgcct
      541 ttgcacggat acacgccgat cggcggattg ccgccgccaa ccgtgcgtgc tccggcgcgc
      601 ggggcgggct tgccggtgcg ttccatgtag gtaaaggaat tgcggatata gtgcaggatg
      661 gcgtcctgca tcgccagttg cagatgctcg ccctggccgg tgcgcagccg ccgcacatag
      721 gcgcccagca cggtgatcgc catcagcatg ccggtgccgg tgtcgccgat cgtcgcaccc
      781 ggcttgcagg gcggcccgtc ctcctcgccg gtgatgctca tcagcccgcc ggcggcctgc
      841 gcgatcatgt cgaaggccag cgacttctcg aacgcgctgc cggcgccgaa gcccttggtc
      901 tggcaataga tgatcgccgg attgatcgcc cggatcacct ccggccccag gccgagccgt
      961 tcgatcgcgc cgggtgcgaa gttctcgcag aacacgtcgg cggatttcgc catctgcttg
     1021 accagttcga cgccgcgcgg gtccttcagg ttgatggtga ccgacttctt gttggcgttg
     1081 tattgcagga agtagaacgc atccgtgccg ggctgcccgc cggcggtgcg gccgggatcg
     1141 ccaaccttgg ggttctccac cttcaccaca tccgcgccca tccaggcgag cgcttcggtg
     1201 caggacggac ccgcctcgaa ttgcgtgaga tcgaggactt tcatgccggt caggcatgtc
     1261 tccgggccgc tgccgttcat tcttggacac tccccctttg caggtatcgg gaaagcttag
     1321 gcccgccggc ggaagggcgt aaatccggcc ggctggctgg cggagatcac cgcgccacga
     1381 gcgccgcgcc aagcgccagc aaggccagga agaacacccg ccggaagctt gcctgtgcaa
     1441 tccggttgcg cagcctctgc cccagcgcca tgccggccaa ggcaggcagg atgcaggcga
     1501 gtgactcgcc tgccgccgtc acctgcaatg cgccgcgggt cgccaatccc gccgcaagcg
     1561 acagggtcga gacggtgaag gacagtccca gtgcctgcac cagcacgtcc ttcttcagat
     1621 ccagcgcttg caggtagggc acggcgggca gcacgaacac gccggtcgcg ccggtgatca
     1681 cgccggttgc caggccggcg gccggcgaca gccatcgctc atggtttggc cgcacggcaa
     1741 ggcgcaggcg cgcgaggccg aacaggccat aggccagcag cgccccgccg agtgccagcg
     1801 tggcgtcccg ggccacgccg ttcaccagca ggccagcccc cgcccaggtc gcggcgcaga
     1861 tcgcgagcag catcggccac aggcggcgca gcaatcccag ccggtgcggg ccgccgaggt
     1921 actgccacag attggtggcg agcgacggga acacgatcag ggatgcggct tccgccggcg
     1981 gcatctgcaa gcccagcagg cccatcgcca cggtcggcaa tcccatgccg gcaacaccct
     2041 tggccgatcc agccaacagg aaactgaacg cggcgaacaa cagcggggcg gaaagcgaat
     2101 gcatggcggc aagctggccg gcatgccgca gactgacaat gcggaagttc cgcgctatgg
     2161 cttcggtgca gccgaaggca caaggggcgc ggaatgcact ttgacctggt cgatctgcgg
     2221 ttgttcgccc gggtggcgga aaccgccacg atcacgcaag ccgcggcaat gtccggactc
     2281 gcgctggcct cggcgagcga gcgtatccgc ggcatggagg cgcaggccgg cgtcgccctg
     2341 ctgcggcgcg ggcgccgcgg cgtgaccctg acgccggccg gacacgcgct ggcgcatcac
     2401 gcgcgcatgg tgctgcagca gttcgaccag ttgaccggcg cattgtccaa atatgccggc
     2461 gggctgcgtg gcttcgtccg gctggccgcc aacggctctg cctgcagcga attcctgccg
     2521 gagcggctca gcctcttcct gcgcgacaat ccgggaattg atgtcgatct cgaggagaag
     2581 cccagcttcg acattgtgcg cctggtcgcg gagggcttcg ccgatatcgg ggtggtggcg
     2641 gacatcgtgg atttcgccgg gctgcaggca tttcccttcg cacgcgaccg gctggttgtg
     2701 gtgttgccgc gtgcgcaccc gcttgccggg cagggagacc gccacttccg cgacctgctg
     2761 gggcacgagt tcattggcat gccggcgggc aatgcactgc aacagcatgt gtcgcagcac
     2821 gcggcgcagg cgggccggcc attgaagctg cgcatccggc tgaacagttt cgaggcgatc
     2881 tgccgcatgg tggaaaccgg cgtgggaatt gccgtgctgc ccgaaaccgc ggcacgccga
     2941 tgccgccggt cgatggccat caccacatgc cggctggcgg atggctgggc gttgcgccaa
     3001 ctgcacgtct gcctccggtc cgcgccggac ctgccggggc cggcgcgacg gctcgccgag
     3061 cacctcattg accggggcac gcgtcccggc cgctaacgcg tcgtcccgga cctgacgttc
     3121 gttctgaccc gctccgcgga gcctcgcttc ccccgcgtca tggggggcgc aggcccgtca
     3181 tccacgactg gctgggaaac tgtcgaagcc gcttgcgcgt gcgataccaa cctgccgagc
     3241 aaatgacaca ccgggcgcag tatcacgtc
//
LOCUS       DASSZH010000490         3471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1477584, whole genome shotgun sequence.
ACCESSION   DASSZH010000490 DASSZH010000000
VERSION     DASSZH010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477584"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VFN42_10730"
     CDS             <1..237
                     /locus_tag="VFN42_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997129.1"
                     /translation="SATALANGTEEDQEHQATRMTDGNDKESAVAELHGTLANITLAL
                     VIAHVLGVGLASFVHRENLVLAMVTGRKRARDEG"
     gene            360..1808
                     /locus_tag="VFN42_10735"
     CDS             360..1808
                     /locus_tag="VFN42_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET8997130.1"
                     /translation="MIQLPDPKPEILARRDAIIAGLRPLLAEEAVIAEPIRLKPYETD
                     GLSAYRQMPLAVVLPETTEQVAAVMRYCHQNGVRVVPRGAGTSLSGGALPLADAVVVG
                     LMRMNRILDIDYADRCATVQAGVTNIGITNAVAAQGFFYAPDPSSQLACMIGGNVNMN
                     SGGAHCLKYGVTANNLLGLKIVTVDGDILSIGGGHLDAAGYDWLGLLTGSEGQLALIT
                     EVTVRILRGPEGARAMLAAFRSNEVAGACVDSIISSGIIPVALEFMDRPAIHACEDFA
                     HAGYPLDAEAMLIIEVEGSPQEQDELLERISAICRKHDPISLKVSQSAEESAAIWKGR
                     KGAFGAVGRISPDYLCMDGTIPTSQLPYVLRRIGEMSDACGLKVANIFHAGDGNLHPL
                     IMFDANDPASFHRAEQFGADILKLCVEVGGCLTGEHGVGVEKRDLMPVQFAAHELEQQ
                     RRIKAAFDPGWLLNPHKVFPLPPQAEPVRDAA"
     gene            1826..3043
                     /gene="glcE"
                     /locus_tag="VFN42_10740"
     CDS             1826..3043
                     /gene="glcE"
                     /locus_tag="VFN42_10740"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HET8997131.1"
                     /translation="MDARTHAPPADPGEAACAAAIAEAASASEPLLVCGNGTKQAMLR
                     PVQAARSLSTRHHSGITLYAPKELIISARAGTPLPEIEAALTEHGQHLIAEPPDYSAL
                     LGAPDRPQTIGGMIATNLSGPRRVAWGAMRDHLMGVRAVTGWGEIIHSGGRVLKNVTG
                     LDLCKLLAGSHGTLAVITEVTLKVLPAPEAVGTLVLSGLDAAAGVAALSAALGSPYSV
                     SGAAWLPAEAAARLPGLPPGRSATLIRIEDFAASVSYRTGKLRQELGFSPADILDDAA
                     SRPLWRAVRDARPLAAPPEHAVWRVSVRPSSGPHILQAVERSGAGGFLDWGGGLVWLA
                     GPATATTHDAIAGAARAAGGTWTLLRAPDTLRTAVAVVPPEPEPLARITRRMKAALDP
                     RGVLNPGRIYAGL"
     gene            3043..3327
                     /locus_tag="VFN42_10745"
     CDS             3043..3327
                     /locus_tag="VFN42_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997132.1"
                     /translation="MIIPSGIIMPLFFVGFAMTCYQYYHIDPRRRVLNLRFAIMAVTT
                     VGAVSFPVVQYLYGQKALLAPVSQGYTAAGVLCLVLSYILLKRMPPAEHY"
     gene            3329..>3471
                     /locus_tag="VFN42_10750"
     CDS             3329..>3471
                     /locus_tag="VFN42_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase iron-sulfur subunit"
                     /protein_id="HET8997133.1"
                     /translation="MQTNFTPEQLRDPDTAASNQVLRTCVHCGFCTATCPTFVLLGDE
                     LDSP"
BASE COUNT          537 a         1261 c         1128 g          545 t
ORIGIN      
        1 agcgcaaccg cccttgccaa cggcaccgag gaagaccagg aacatcaagc cacgcgcatg
       61 accgacggca atgacaaaga gagcgcggtc gccgagttgc acggcacgct cgccaacatc
      121 accctggccc tggtgatcgc ccatgtgctg ggcgtcggcc tggcgagctt cgtgcatcgt
      181 gagaacctgg tcctggcgat ggtcaccggc aggaagcggg cgcgcgacga aggctgacgg
      241 gcggcggcgg cccctccgcg caccggcgat cgcctagcgg cgttgccggt ggcgaaaggt
      301 tgacctttcg cttgccccgc cccctgcccc gcgccatgtt ccggccggag aacccagtca
      361 tgatccagct tccagacccc aagccggaaa tcctcgcccg ccgcgatgcc atcatcgccg
      421 gattgcggcc gctgctcgcc gaggaagcgg tcatcgccga accgatccgc ctgaagccat
      481 acgagacgga cggcctgtcg gcctaccgcc agatgcccct tgcggtcgtg ctgccggaaa
      541 ccaccgagca ggtcgccgcg gtgatgcgct actgccacca gaacggcgtc cgcgtggtgc
      601 cgcgcggcgc cggcacaagc ctgtccggcg gcgcgctgcc gctggcggat gccgtggtgg
      661 tcggcctgat gcggatgaac cgcatcctgg acatcgacta cgccgaccgc tgcgccacgg
      721 tgcaggccgg ggtcaccaat atcggcatca ccaacgccgt cgcggcgcag ggtttcttct
      781 atgcccccga cccgtcgtcg cagctcgcct gcatgatcgg cggcaatgtc aacatgaact
      841 ccggcggcgc ccactgcctg aaatacggcg ttaccgccaa caatctgctc ggcctgaaga
      901 tcgtcacggt ggacggcgat atcctcagca tcggcggcgg ccatctggat gccgccggct
      961 acgactggct cgggttgttg accggcagcg agggacagct cgcgctgatc accgaagtca
     1021 ccgtgcgcat cctgcgcggc ccggaagggg cccgtgccat gctggcagca ttccgctcga
     1081 atgaagtagc aggcgcctgc gtcgactcca tcatctcctc cggcatcatc ccggtggcgc
     1141 tggaattcat ggaccggccg gcgatccacg cctgcgagga cttcgcgcat gctggctacc
     1201 cgctggacgc cgaggcaatg ctgatcatcg aggtggaagg cagcccgcag gaacaggacg
     1261 agctgctgga gcgcatcagc gcgatctgtc gcaagcacga cccgatcagc ctgaaagtgt
     1321 cgcagtcggc ggaggagtcg gcggcaatct ggaagggccg caaaggcgcg ttcggcgcgg
     1381 tcggccggat cagcccggac tatctgtgca tggacggcac catccccacg tcgcagctcc
     1441 cctacgtgct gcgtcggatc ggcgagatgt cggatgcctg cgggttgaag gtggccaaca
     1501 tcttccacgc cggcgatggc aacctgcatc cgctgatcat gttcgacgcc aacgatccgg
     1561 ccagcttcca tcgcgccgag cagttcggtg cggatatatt gaagctgtgc gtggaggtgg
     1621 gcggctgcct caccggggaa cacggcgtcg gcgtggaaaa gcgggacctg atgccggtgc
     1681 agttcgcagc gcacgagctg gaacagcagc gccgcatcaa ggccgcgttc gatcccggtt
     1741 ggctgctgaa cccgcacaag gtgttcccgc tcccaccgca ggcggaaccc gtccgagacg
     1801 cggcgtgacc gcggcagccg ttatcgtgga cgcaaggacc catgcgcctc ccgccgatcc
     1861 gggtgaagcc gcctgcgctg ccgccattgc agaggccgcc tcggcaagtg agccgctgct
     1921 ggtctgcggc aacggcacca agcaggccat gctccgtccg gtgcaggcgg cgcgcagcct
     1981 ctccacccgc caccacagcg ggatcacgct gtacgcgccg aaggagctga tcatctccgc
     2041 ccgcgccggt acgccgctgc cggaaatcga ggccgccctg accgaacacg gccagcacct
     2101 gattgccgaa ccgcccgact attcggccct gctgggcgcc ccggaccggc cgcagaccat
     2161 tggcggcatg atcgcgacca acctgtccgg cccccgccgc gtcgcctggg gcgccatgcg
     2221 cgaccacctg atgggcgtcc gcgccgtcac cggctggggg gagatcatcc attccggcgg
     2281 ccgcgtcctg aagaacgtca ccgggctcga cctgtgcaag ctgctcgccg gcagtcatgg
     2341 cacgcttgcg gtcatcaccg aggtcacgct gaaggtgctg ccggcgccgg aagccgtcgg
     2401 cacgctggta ctgtccggcc tcgatgccgc ggccggcgtc gccgcgctat ccgccgcgct
     2461 tggctcgccc tacagcgtgt ccggcgccgc ctggctgccg gccgaggccg ccgcgcgcct
     2521 gcctggcctg ccccccggcc gctccgccac cctgatccgc atcgaggact ttgccgcctc
     2581 cgtgagttac cgcaccggga aactgcgcca ggagctggga ttcagtcccg ccgacatcct
     2641 ggatgacgcg gcatcccgcc cgctttggcg cgcggtgcgc gacgcccggc cgctggccgc
     2701 gccacccgag cacgcggtgt ggcgcgtctc ggtgcgtccc tccagtggtc cgcatatcct
     2761 gcaggcggtg gaacgctctg gcgcgggcgg tttcctcgac tggggcggcg ggctggtctg
     2821 gctggccggg ccggccaccg ccaccacgca cgacgccatc gccggcgccg cccgggctgc
     2881 cggcggcacc tggaccttgc tgcgtgcgcc cgacacgctg cgcaccgcgg tcgcagtggt
     2941 gccgcccgag ccggagccgc tcgcccgcat cacccgtcgg atgaaggccg cgcttgatcc
     3001 gcgtggcgtg ctgaatcccg gccgcatcta cgcggggctg tgatgatcat tccctccggc
     3061 atcatcatgc cgctgttctt tgtcggtttt gccatgacct gttaccagta ctaccacatc
     3121 gacccgcgcc gccgggtgct gaacctccgc ttcgccatca tggccgtcac gacggtcggt
     3181 gcggtcagct tccccgtggt gcaatacctg tacggccaga aggcgctgct cgcgccggtg
     3241 tcacaaggct acaccgccgc cggcgtgcta tgccttgtgc tgtcctacat tcttctgaag
     3301 cgcatgccgc cggctgaaca ctactgacat gcagaccaac ttcaccccgg agcagttgcg
     3361 cgatccggac accgccgcgt ccaaccaggt gctgcgcacc tgcgtgcatt gcggcttctg
     3421 caccgccacc tgccccacct tcgttctgct cggcgacgag ttggacagcc c
//
LOCUS       DASSZH010000491         5209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_778799, whole genome shotgun sequence.
ACCESSION   DASSZH010000491 DASSZH010000000
VERSION     DASSZH010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778799"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VFN42_10755"
     CDS             complement(<1..251)
                     /locus_tag="VFN42_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997134.1"
                     /translation="MPSGLEGVVAAETVLSHADPQTGMQWVRGHPLPELVARYGYEGA
                     AGLLWEDFAGQDLTRSTVQSLLGEARVAAFTALPCWLPQA"
     gene            complement(325..1332)
                     /locus_tag="VFN42_10760"
     CDS             complement(325..1332)
                     /locus_tag="VFN42_10760"
                     /inference="COORDINATES: protein motif:HMM:NF014789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET8997135.1"
                     /translation="MGTTQEAAAKVDAWLSRCADPATDPLQGMAETGLLEPETSYARI
                     ARIKALLVERTGLLGVGGIWAGRQVVNRWFLQGFGTTAQRAAWLGHAASVAISEPGAG
                     AHPKRLTTHADADGEGYCISGAKAWVSNGPSAEVFVVLAITAREGERKRYSAFLVPHD
                     APGLSLQEMPAFHALRPSRHCGLRLDRVPVARDALLGTAGNAYEAMALPFRDVEDAVG
                     TLGLLGAFRFLLRALSGSAESDEAAAMLGGLAASAAVFEDAADALLAALDAGRLNDKA
                     ATLVGLRVLATDMTQRFRAYVQQCGRLGDPAIEDVLRDIDATLSVARGPRLARQIRLA
                     G"
     gene            complement(1363..2085)
                     /gene="phbB"
                     /locus_tag="VFN42_10765"
     CDS             complement(1363..2085)
                     /gene="phbB"
                     /locus_tag="VFN42_10765"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HET8997136.1"
                     /translation="MARVALVTGGTRGIGSAISAALQAQGRTVVANYAGNDDAARRFS
                     ESTGIRVWKFDVGKFEQCQEAVQQIAAEVGPIEILVNNAGITRDGTLGRMSREMWDAV
                     IDTNLGSCYNLCKLTFDAMRAAKFGRIVNVGSINGQAGQYGQVNYAAAKSGIHGFTKA
                     LAQEGARYGITVNAIAPGYVDTDMVRAVPDDVLQKIIARIPVGRLGQAADIARGVTFL
                     TAEDADFITGSTLSINGGQHMY"
     gene            2291..3127
                     /locus_tag="VFN42_10770"
     CDS             2291..3127
                     /locus_tag="VFN42_10770"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HET8997137.1"
                     /translation="MTAEPGSTGLLARLMPWRRPRLNVVELHGLIAPRQEALSASSVG
                     PLIDRAFRTAKGRPVILDIESPGGSPVQSDLIAALIRRRAEKHKVRVVAVIREVGASG
                     GYWLACAADEIHANPMSIVGSIGVRGGGFGVADLLVRLGVEHRLYTAGANKARFDPFS
                     PERPEDVAFAREMLDALHERFKEWVRSRRGTRLKAEEAAVFDGSFMLGEQALALGLID
                     GLNDLDALVRQLGGERAVARRFSFRRRGLLRRLPRLALEMVLDVVEDRAWRARLPGAR
                     LL"
     gene            3187..4392
                     /locus_tag="VFN42_10775"
     CDS             3187..4392
                     /locus_tag="VFN42_10775"
                     /inference="COORDINATES: protein motif:HMM:NF016552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997138.1"
                     /translation="MTRCRGGATPVQALGAPLERFTKKSGHRVGKERMKLVRASGLFG
                     ALLLLVTTAMPIGRAYASPVYNGGPILTGVPNVYYIWYGDNWSSDTATTILPNLITNL
                     SGTPYMNIATSFSTGSYTVGNKVNFGGSVFVNSSSNSGIYTTNTTSLDGVQLDTGNRT
                     IFGIVQQVLAANSTWAADPNGIFDVLTAPGITVSGFGAGGFCGWHYSGSVATGTLNWG
                     GSHLATQYGFIGDPLQGGGCNVSLQNSPTANGNFGADAMASVIAHELFETITDPTGRA
                     WFDSVNGSSTNGLEGADMCAWNFGTVSNSGGANYNVTMNGTNYLLQQQWLNEGGGTGT
                     TGGVCTMSLAMSTPVPEPASFLVLLPGLLGLAGLRWRRAGWSSPSDGGNRPHGSCRTA
                     TPHRPAYVV"
     gene            4595..>5209
                     /locus_tag="VFN42_10780"
     CDS             4595..>5209
                     /locus_tag="VFN42_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET8997139.1"
                     /translation="MNQTATAASAGDPQRMSTGEAAVASLEANGIDTVFCLPGVQNDY
                     LFDALHGAQDRIRAIHTRHEQGAAYMALGAAMATGKPAVYAVVPGPGFLNTTGALCTA
                     YAVNAPVLAMVGQIPAAAIGRGYGLLHEIPDQLGTMRSLSKYAERIRAPHEAPLKVNE
                     AFRQMMSGRPRPAALECAIDVWSRRAPVAIPAAAQADPMPVDPEA"
BASE COUNT          861 a         1720 c         1751 g          877 t
ORIGIN      
        1 gcctgcggca gccagcacgg caacgccgtg aaggccgcca cgcgtgcctc gcccagcagg
       61 gactgcaccg tgctgcgggt caggtcctgc ccggcgaagt cttcccacag cagcccggcc
      121 gccccttcgt agccataccg ggcgacgagt tcgggcaacg gatgcccgcg cacccattgc
      181 atcccggttt gcgggtcggc gtggctgagc acggtctccg ccgcgacaac gccttcgagt
      241 cctgacggca tgctgcgtcc catccggttt cggcggcagg atgatggttc cgcgcggtag
      301 cgtccaatgc agccgtcagg cggctcaacc tgccagccgg atttgccgag ccaggcgtgg
      361 accgcgggcg accgacaggg tggcgtcgat gtcgcgcagc acgtcctcga tcgccggatc
      421 gccgagccgg ccgcattgtt gcacgtaggc gcgaaagcgc tgcgtcatgt cggtggccag
      481 aacgcgcagg ccaaccagcg tggcggcctt gtcgttcagc cgccccgcat ccagcgcggc
      541 caacagcgcg tccgccgcgt cttcgaacac cgcggccgag gccgccaagc cgcccagcat
      601 cgcggcagct tcgtcggact ccgcggaacc ggagagcgcg cgcagcagga agcggaacgc
      661 cccgagcaga ccaagcgtcc ccacggcatc ttccacgtcg cggaacggca aggccatggc
      721 ttcataggcg ttgccggccg ttcccagcag cgcgtcgcgg gcgacgggaa cgcgatcgag
      781 acgcaggccg caatgccgcg aagggcgcaa ggcgtgaaat gccggcatct cctgcaagga
      841 caggcccggc gcatcgtgcg gcacgagaaa ggcgctgtag cgcttgcgtt cgccttcgcg
      901 tgcggtaatg gccagcacga cgaatacctc ggcggagggg ccgttgctga cccaggcttt
      961 cgcaccgctg atgcaatagc cctccccatc cgcgtcggca tgcgtggtca gccgcttggg
     1021 atgcgcgccg gcacccggtt ccgagattgc caccgacgcc gcgtggccca gccacgccgc
     1081 gcgttgtgcc gtggtgccga acccctgcag gaaccagcga ttgacaacct gccgtccggc
     1141 ccagatgcca ccgacaccca gcagcccggt gcgctccacc agcagcgcct tgatgcgggc
     1201 gatccgggca tagctggttt ccggctccag caacccggtt tccgccatgc cctgcaacgg
     1261 atcggtggcg ggatcggcac atcgggacag ccaggcatcg accttggctg ctgcttcctg
     1321 tgtcgtgccc atttgccgcc gaaccgccgt tgccgcgccg attcagtaca tatgctgccc
     1381 gccgttgatc gacagcgtgg agccggtgat gaaatccgcg tcctccgcgg tgaggaacgt
     1441 cacgccgcgc gcgatatcgg cggcctggcc aagccggccc accgggatgc gcgcaatgat
     1501 cttctgcagc acgtcgtccg gaaccgcgcg caccatgtcg gtatcgacat agccgggggc
     1561 gatggcattc accgtgatgc cgtagcgcgc gccttcctgc gccagcgcct tggtgaagcc
     1621 atggatgccg gatttcgccg cggcatagtt gacctggccg tactgcccgg cctggccgtt
     1681 gatgctgccg acgttgacga tgcggccgaa cttcgccgcg cgcatcgcat cgaacgtcag
     1741 cttgcacagg ttgtagcagg aaccgagatt ggtatcgatc accgcatccc acatctcgcg
     1801 gctcatgcgg cccagcgtcc cgtcgcgggt gatgccggcg ttgttcacca ggatctcgat
     1861 cggtccgact tcggcggcga tctgctgcac ggcttcctgg cactgctcga acttgccgac
     1921 gtcgaacttc cagacgcgga tgccggtgct ctcggagaag cggcgggcgg catcgtcatt
     1981 gcctgcataa ttggcaacga ctgtgcgtcc ctgcgcctga agcgcggcgc tgatcgctga
     2041 gccgatgccg cgtgtcccgc cggtgaccag tgcgacgcgt gccatgggcg ttccctcctg
     2101 ctgcctgtag cgttcgacag gattgatgtt agcacgtttg cgtggaaaac ccaggccata
     2161 ccggccggtg acctgcccaa atcgaccggc gccctgtatg cgcctggtac ttgccggtat
     2221 tgaagcctgc cgctgcgcaa tggccgcggt ccacaccata tcgctggcga ccacgtaagg
     2281 agagatttgc atgacggccg aaccgggctc caccggactg cttgcacgcc tgatgccctg
     2341 gcgccgcccg cggctcaacg tggtcgaact gcacgggctc atcgcgccgc ggcaggaggc
     2401 gctcagcgct tcgtcggttg gcccgctgat cgaccgtgcc ttccgtaccg ccaaggggcg
     2461 gccggtgatc ctggacatcg aaagcccggg tggctcgccg gtgcagtcgg acctgatcgc
     2521 cgccctgatc cgccggcgtg cggaaaagca caaggtgcgc gtggtggcgg tgatccgcga
     2581 agtgggcgcg tccggcgggt attggctcgc ctgcgcggcg gacgagatcc atgccaatcc
     2641 gatgagcatc gtaggatcca tcggcgttcg cggcggcgga tttggcgtcg ccgacctgct
     2701 ggttcggctg ggcgtcgagc accggctgta taccgcaggt gcgaacaagg cgcggttcga
     2761 tccattcagc ccggaacggc cggaggacgt ggccttcgcg cgcgagatgc tggatgcgct
     2821 gcacgagcgc ttcaaggaat gggtccgcag ccggcgcggg acgcggctga aagccgagga
     2881 agccgctgtg ttcgatggca gcttcatgct gggggaacag gcgctggcgc tggggctgat
     2941 cgacgggctg aacgacttgg acgcgctggt gcgccagctt ggcggggagc gcgcggtggc
     3001 gcggcggttc tccttccgcc ggcgcggcct gctgcgccgg ctgccgcggc tggcgctgga
     3061 gatggtgctg gatgtggtgg aggaccgggc ttggcgagcc cggctgccag gggcgcggct
     3121 gctttagcgc cgttgggtgc ggacgttcgg ttccgccgaa cgggggagcg gctcacggat
     3181 tgttgaatga cccgttgccg aggcggtgcg acgccggtac aagcattggg tgcgccgctg
     3241 gagcggttca caaagaagtc cggccaccgg gtgggaaaag aacggatgaa gctcgtgcgc
     3301 gccagtgggt tattcggggc gctgttgctg ctggtaacca cggcaatgcc gattggccgc
     3361 gcgtacgcat cgcccgttta caacggcggg ccaattctta caggggtgcc aaacgtttac
     3421 tacatttggt acggcgacaa ttggagctcg gacacggcga cgaccatcct gccgaacctg
     3481 atcaccaatc tcagcggcac gccgtacatg aatattgcaa cgtccttttc taccggctcc
     3541 tacaccgtcg gcaataaggt caatttcggc ggcagcgttt tcgtcaacag cagcagcaac
     3601 agcggaatct acaccaccaa caccacaagc ctcgacggcg tgcaactgga caccggcaac
     3661 aggacgatct tcggtattgt tcagcaggtg ctggccgcaa acagcacgtg ggcagcggac
     3721 ccgaatggta tttttgacgt actgacggcg cccggcatca cggtaagcgg gttcggcgcc
     3781 ggtggcttct gcggctggca ctattccggg tcggtcgcga ccggtacatt gaactgggga
     3841 ggctctcatc tggcaaccca gtatggattt atcggcgatc cgctgcaggg tggcggttgc
     3901 aatgtctcgc tccagaattc cccgaccgcc aacggcaatt tcggcgccga tgccatggcg
     3961 agcgtgattg cgcacgaact gttcgaaacc atcaccgatc cgaccggccg cgcctggttt
     4021 gatagtgtta acggttccag tacgaacggg cttgagggcg cggacatgtg cgcgtggaac
     4081 ttcggcacgg tctcgaacag cggcggtgcg aactacaacg tcaccatgaa cggcaccaac
     4141 tacctactgc agcagcaatg gctcaacgaa ggcggcggca ctggcaccac gggcggtgtc
     4201 tgcaccatga gtctcgccat gagcacgccc gtgcccgaac ccgcgagctt cctggtgctg
     4261 ttgcccggcc tgctcggtct ggccggcctg cgctggcggc gcgccggctg gtcctcgccg
     4321 agcgacggcg gcaatcgtcc ccatggctcc tgccggacag caacgccgca tcggcccgca
     4381 tacgtggtct gatccgcatt ctgctgccga agatcgccac cagcctggca gaggccccgg
     4441 tggccgggct tggccagcac cggacaagtg ccgcatgccg gcgatgttcg gcacaggcgt
     4501 ttcatctccc tgccgatgcc ggtgaccggg cgaagacttc cggcggcacc ggctgcttgc
     4561 tagcctgcca gcaaatcggt gaaggggaaa cgacgtgaac cagacggcca cggcagcatc
     4621 cgccggcgat ccgcaacgca tgagtaccgg cgaggctgca gttgcgtcgc tggaggccaa
     4681 tggcatcgac acggtgttct gccttcccgg cgtgcagaac gactatctgt tcgatgcgct
     4741 gcatggtgcg caggatcgca tccgcgccat ccatacgcgg catgagcagg gcgccgccta
     4801 catggcgctc ggcgcggcaa tggcgaccgg caagccggcg gtatacgccg tggtaccagg
     4861 tccgggcttc ctgaatacca ccggcgcgct gtgcaccgcc tatgcggtga atgcgccggt
     4921 cttggcgatg gtgggccaga ttcccgccgc cgcgatcggc cggggctacg gtctgctgca
     4981 cgaaatcccc gaccagctcg gcaccatgcg ttcgctgagc aaatacgccg aacggattcg
     5041 tgcgccgcac gaggcgccgc tgaaggtgaa tgaggcattc cgtcagatga tgtccggccg
     5101 cccgcggccg gcggcactgg aatgcgccat cgacgtatgg tcgcgccgtg caccggtggc
     5161 gattcccgct gcggcgcaag ccgatccgat gccggtcgac cccgaggcc
//
LOCUS       DASSZH010000492         5339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1699476, whole genome shotgun sequence.
ACCESSION   DASSZH010000492 DASSZH010000000
VERSION     DASSZH010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1699476"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VFN42_10785"
     CDS             <1..441
                     /locus_tag="VFN42_10785"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8997140.1"
                     /translation="EEWDAVLRTNLSASFYLARHAIPHMRTRNWGRVIVISGADGFWG
                     HVTHRAPNLAAKSGMHGLAKAIAREFGADGITANTVSPGPIDTVRDWSQYVHQPSERI
                     TAEVPLGRYGHVDEIAGACLFLASRAGGYVSGQVLHVNGGHNMY"
     gene            446..1459
                     /locus_tag="VFN42_10790"
     CDS             446..1459
                     /locus_tag="VFN42_10790"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HET8997141.1"
                     /translation="MLRLLADDLTGALDSAARFVPLTGPLPVVWSGDAPAGHAALDSG
                     TRNLDAAAAAERVAALAPLLADGAPAFKKIDSLLRGHVAVELAACMRWFDRCILAPAF
                     PFQGRITIGGRQLVQDGDGWRDTGLDLAAALRAHGVDAELCDAATDAELDAIVAAGRL
                     LPGRVLWCGTGGLAGALAETRDAPCPPLPGPVLALIGSDHAVALAQLTAVGDRLHRVT
                     EPDAATISALRASLEGGAAAVCLALPPHALRAEAADRIGACFAALLHALPRPGTLVVA
                     GGETLRDLCRRLGVSRLEVDGEVVPGVPTSRFCGGAWDGQRVVSKSGAFGAAGFLAEL
                     LGG"
     gene            complement(1496..1957)
                     /locus_tag="VFN42_10795"
     CDS             complement(1496..1957)
                     /locus_tag="VFN42_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HET8997142.1"
                     /translation="MTSVPEWVWSLVMAVHILCAVVWVGGMFFAYMVVRPSLVAIDPP
                     QRMLLHTQVFRRFFLVVWHAMPLILLSGFAMVLWQFGGMAHLRWNINAMMGIGLLMSL
                     IFVYIVFGPYKRFRRTTDRTRMASSLDSIRKLIGLNMLLGLITVVLAAWNW"
     gene            complement(1954..2583)
                     /gene="parA"
                     /locus_tag="VFN42_10800"
     CDS             complement(1954..2583)
                     /gene="parA"
                     /locus_tag="VFN42_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family partition ATPase"
                     /protein_id="HET8997143.1"
                     /translation="MAVVITVAQQKGGTGKTTLAANLAAALAPTRRVALLDIDPQKSL
                     TRWHALRRARLQEAAALTFSDVSGWRLAAELDRLKQAHDVVLVDSPPQIDTDARLAVR
                     GADFVLVPVQPSPPDVWAAEGTLALAAAEGRPAHLVLNRVPPAGKLRGSVAEDLANRK
                     LPLLRTAIGNRTGFAAAFAEGLGVTEAAPRSTAASELRALLDELLEMTQ"
     gene            2707..3570
                     /locus_tag="VFN42_10805"
     CDS             2707..3570
                     /locus_tag="VFN42_10805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HET8997144.1"
                     /translation="MDYRTRFIVEPGAKLRLGKIDPSFAGNTDHEAARDEIARHVQRL
                     DALQYLLYADHQQSLLIVLQALDAGGKDGVIRHLFSGMNPQGTAAFSFKQPSQAERDH
                     DFLQRAHLRAPGKGEVVIFNRSHYEDVLVVRVHKLVPRAVWSKRYALINDFEAMLAQN
                     GTRILKFYLHISPEEQLERFRQRLEDRTRHWKISESDYLERKFWPQYIEAYEDALMRT
                     STRQAPWYVIPANHKWFRNLAISQIVTRTIEEMGLNLPPTQVDLADIRRRYHEAAAAQ
                     SENHQAKGRHA"
     gene            3563..5329
                     /locus_tag="VFN42_10810"
     CDS             3563..5329
                     /locus_tag="VFN42_10810"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HET8997145.1"
                     /translation="MPEAAARPGLLFASLRGFRFSWLPGDAIAGLTLAAIAIPEQLAT
                     ARLLGMPPVSGLFAFAAGTIAFAAFGTNRFVSVGADSTIAPIMAATLASLAAIGSPHY
                     AEAAAVLALLTGLVLLLTRPLRLGWIADLLSIPVATGFLAGISIHIIIGQLPTILGVA
                     APDGALLDRLGAIVRMLPRTNPYPLAIAAGVLAVSMAGELINARLPGALIGLAGSACA
                     VWGLALQHHGVPVLGPLPFAIPTPAFALPDLNLLLRLLPLSLIVALVCMVQTAVVLQS
                     FPSDRDHPGDASRNFLAVGAGSILAALLGGFAVNASPPRTAVVAESGGRSQFAGLLAL
                     GFVAALVLLGARAFAFVPYAALSGVLVFIALRIFRLATMRQIYRRSREETLLVAASAA
                     LVVVLPIETGVSMSIVLSLLHSLYIIARPRCAELTRVPGSTVWWTLGPGEAGEHVPGV
                     LVFGFGAPIAFINANYLVERLSQALQAAPAPVRLVVIEAHGVLDVDFTGSLILQQAIV
                     QARRNGIDVAIARLESMRAMQAAERTGLLAVLGPDHVFRSVEEAINAIVTPGTMQLPG
                     NGRVDRPAAGRRDARDLGARHT"
BASE COUNT          796 a         1825 c         1862 g          856 t
ORIGIN      
        1 gaggagtggg atgcggtgct ccgcaccaac ctgtccgcgt cgttctacct ggcccgccac
       61 gccatcccgc acatgcgtac gcggaactgg ggacgggtaa tcgtgatctc cggtgccgac
      121 gggttctggg gccatgtcac acaccgtgcg ccgaacctcg ccgcaaaatc cggtatgcac
      181 ggactggcca aggcgatcgc acgcgaattc ggcgccgacg gcatcaccgc gaacacggtg
      241 tcgcccgggc cgatcgacac ggtgcgcgac tggtcgcaat acgtgcatca gccaagcgaa
      301 cgcatcaccg ccgaggtgcc gctcgggcgc tacggccacg tggacgagat cgccggcgcc
      361 tgcctgttcc tggcgagccg cgccggcggc tacgtctccg gacaggtgct gcacgtcaac
      421 ggcggccaca acatgtattg accccatgct acgtttgctt gccgacgacc tgaccggagc
      481 cctggacagt gccgcgcgct tcgtgccgct gaccggaccg ctgccggtgg tttggtcggg
      541 cgatgcgcca gccggccacg ccgcgctcga cagcggcaca cgcaacctgg acgccgcggc
      601 ggcggcggag cgggtcgccg cgctggcgcc gctgctcgcg gacggcgcgc cggccttcaa
      661 gaagatcgac agcctgctgc gcggccatgt cgcggtggag ctcgccgcct gcatgcgctg
      721 gttcgaccgc tgcatcctgg cgccggcgtt cccgttccag ggccgcatca cgatcggcgg
      781 gcgccaactg gtgcaggacg gcgacggctg gcgcgacacc gggctggatc tcgccgcggc
      841 cttgcgcgcg catggcgtgg acgccgagct gtgcgacgcg gcgacggacg cggagctgga
      901 tgcgatcgtc gccgccgggc gcttgctgcc cggccgggtg ctgtggtgcg gcaccggtgg
      961 cttggcgggg gctctggcgg agacacgcga tgcgccgtgt ccaccgctgc caggcccggt
     1021 tctggcgctg atcggatcgg atcatgcggt cgccttggcg cagcttacgg cggtcggcga
     1081 ccggctgcac cgggtgaccg agccggatgc cgcgacgatc agcgccttgc gcgcatcact
     1141 ggagggcggt gccgccgcgg tctgcctcgc gctgccgccg cacgcgctcc gcgcggaggc
     1201 ggcggaccgc atcggcgcct gcttcgccgc gctgctgcac gcgctgccgc gtccgggcac
     1261 gctggtcgtg gccggcggtg agacgctgcg cgatctgtgc cggcgactgg gcgtatcgcg
     1321 gctggaggtg gatggcgagg tggtgccggg cgttccgaca tcccgtttct gcggcggcgc
     1381 gtgggacggc cagcgggtgg tgtccaagtc gggcgcgttc ggcgcggcag gtttcctggc
     1441 ggaactgctg ggcggttgac accggcagcc cggctgccca gccaggaacg ctcgcctacc
     1501 agttccaggc cgcgagcacc acggtgatca gccccagcag catgttcaac ccgatcagct
     1561 tgcggatgct gtcgagcgag gatgccatgc gcgtccgatc cgtggtgcgc cggaaccgct
     1621 tgtacgggcc gaacacgatg tagacgaaga tcagcgacat cagcaggcca attcccatca
     1681 tcgcgttgat gttccaacgc agatgggcca tcccgccgaa ctgccagagc accatggcga
     1741 agccggagag caggatcagc ggcatggcgt gccacaccac gaggaagaag cggcggaaca
     1801 cctgcgtatg cagcagcatc cgctgcggcg ggtcgatcgc cacaaggctg ggccgcacca
     1861 ccatgtaggc gaagaacatc ccgcccaccc aaacgaccgc gcacaggatg tgcaccgcca
     1921 tcaccaggga ccagacccat tcgggcacgc tcgtcattgc gtcatctcca acagttcatc
     1981 cagcagcgcc cgcagctcgc tggccgcggt ggagcggggg gccgcctcgg tgacgcccaa
     2041 cccctcggcg aatgcggcgg cgaatcccgt gcgattgccg atcgccgtgc gtagcagcgg
     2101 cagcttacgg ttggcgaggt cctctgccac gctgccgcgc agcttgccgg cgggaggcac
     2161 ccgattcagc accagatggg ccgggcggcc ttccgcggcg gcgagcgcca gcgtgccctc
     2221 ggccgcccac acatccggcg ggctgggctg cacgggtacc agcacgaaat ccgcaccgcg
     2281 caccgccagc ctggcatcgg tgtcgatctg cggcggcgag tccaccagca cgacgtcatg
     2341 cgcctgcttc agccggtcca gttcggcggc caggcgccag ccggaaacat cggagaaggt
     2401 gagcgcggcg gcctcctgca gccgggcgcg gcgcagcgcg tgccagcggg tgaggctctt
     2461 ctgcgggtcg atgtccagca gcgcgacccg cctggtgggc gcaagtgccg ccgccagatt
     2521 ggccgcgagc gtggtcttgc cggtcccgcc cttctgctgc gcgaccgtga tcaccacagc
     2581 catgccgcga ctccgccgtt gcctgggtgc atacaggacc agaaccggcg ctccgcgtca
     2641 cgcggaacag tggatttgcc accacacccg ccggtaacct gtgcgccttg gcaagggaat
     2701 tgctggatgg actaccggac gcgcttcatc gtcgagccgg gcgccaaact ccgcctgggc
     2761 aagatcgacc catccttcgc cggcaacacc gaccacgaag ccgcacgcga cgagatcgcg
     2821 cggcacgtgc agcgcctgga tgcgttgcaa tacctgctgt atgccgacca ccagcaatcg
     2881 ctgctgatcg tgctgcaggc gctggatgcg ggcggcaagg acggcgtgat ccgtcacctg
     2941 ttcagcggca tgaacccgca aggcaccgcc gccttcagct tcaagcagcc gagccaggcc
     3001 gaacgcgacc acgatttcct gcaacgcgcg catctgcgcg caccgggcaa gggcgaggtg
     3061 gtgatcttca accgctcgca ctacgaagac gttctggtgg tacgcgtgca caagctggtc
     3121 ccgcgcgccg tgtggtcgaa gcgctacgcg ctgatcaacg atttcgaggc gatgctggcg
     3181 cagaacggca cacgcatcct gaaattctat ctgcacatca gcccggagga gcaactggaa
     3241 cgcttccgcc agcggctgga agacaggacg aggcactgga agatcagcga gagcgactac
     3301 ctggaacgca agttctggcc gcaatacatc gaggcctatg aggatgcgct gatgcgcacc
     3361 agcacgcggc aggctccctg gtacgtcatt cccgccaacc acaaatggtt ccgcaacctg
     3421 gcgatctcgc agatcgtcac ccggacgatc gaggagatgg gactgaatct gccgcccacg
     3481 caggtggatc tggccgacat ccgccgccgc taccacgaag cggccgcggc gcaatcggag
     3541 aaccatcagg ccaaagggcg ccatgcctga ggcagccgcg cgtcccggcc tgctgttcgc
     3601 gtcattgcgc ggcttccgct tctcatggct gccgggcgac gccattgcgg ggctgacgct
     3661 ggcggcgatc gccataccgg agcaacttgc caccgcgcgg ttgctgggca tgccgccggt
     3721 cagcgggctg ttcgcctttg ccgccggcac catcgccttc gccgcattcg gcaccaaccg
     3781 cttcgtgtcg gtcggcgcgg attccaccat cgcgccgatc atggcggcca cgctggcaag
     3841 cctggctgcg atcggcagtc cgcattacgc cgaagcggcg gccgtcctgg ccttgctgac
     3901 ggggcttgtc ctgctgctga cgcgcccgct gcgcctgggc tggatcgctg acctgctgtc
     3961 gattccggtg gcgaccggct ttctcgcagg catctcgatc cacatcatca tcgggcagtt
     4021 gcccaccatc ctgggcgtgg cggcgccgga cggcgcgctg ctggaccggc tgggtgcgat
     4081 cgtgcggatg ctgccgcgga ccaaccccta tccgctggcg atcgcagccg gcgtgctggc
     4141 ggtcagcatg gccggagagt tgatcaacgc gcgcctgccg ggagcgctga tcggtctggc
     4201 gggcagtgca tgcgcggtct ggggcctcgc gctgcagcac catggcgtgc cggtgcttgg
     4261 ccccctcccc ttcgcgatcc cgacgccggc tttcgcgctg cccgatctga acctgttgct
     4321 gcggctcttg ccgctgtccc tgatcgtcgc gctggtctgc atggtgcaga ccgcggtggt
     4381 gctgcagtct tttccgtcgg atcgggacca tccgggcgac gccagccgca acttcctggc
     4441 ggtgggtgcc ggcagcatcc tggcggcgtt gctgggcggc ttcgcggtga atgccagtcc
     4501 gccgcgcacc gcggtggtgg cggagtccgg cggccggtcg cagttcgccg gcctgctcgc
     4561 gctgggcttc gtggcggcgc tggtgctgtt gggtgcgcgc gccttcgctt tcgtgcccta
     4621 tgcggcgctc agcggcgtcc tggtgttcat cgcgctgcgc atcttccgcc tggcgaccat
     4681 gcggcagatc tatcggcgca gccgggagga gacgctgttg gtcgccgcca gcgccgcgct
     4741 ggtggtggtc ctgccgatcg agaccggcgt cagcatgagc atcgtgctat cgctgctgca
     4801 cagcctttac atcattgccc ggccacgctg tgccgagctg acacgggttc ccggcagcac
     4861 cgtgtggtgg acgttgggtc ccggtgaagc cggcgagcac gtgccgggcg tgctggtgtt
     4921 cgggttcggc gcgccgatcg cgttcatcaa cgcgaattac ctggtggaac ggctgagcca
     4981 ggctctgcag gccgcaccgg cgccggtgcg gctggtggtg atcgaggcac atggcgtgct
     5041 cgatgtcgac ttcaccggct cgctgatcct gcagcaggcg atcgtgcaag cgcgccggaa
     5101 cggtatcgat gtggcaatcg cccggctcga gtcgatgcgg gcgatgcagg cggcggaacg
     5161 aaccggactt ctcgccgtgc tgggcccgga tcatgtcttc cgttcggttg aggaggcgat
     5221 caatgcgatt gtgacgccgg gcacgatgca actgcctggg aacggtcgcg ttgaccggcc
     5281 ggcggccggc cggcgggacg cccgggattt gggagcgcga cacacatgac ggaagacac
//
LOCUS       DASSZH010000493         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1000570, whole genome shotgun sequence.
ACCESSION   DASSZH010000493 DASSZH010000000
VERSION     DASSZH010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000570"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1293)
                     /locus_tag="VFN42_10815"
     CDS             complement(13..1293)
                     /locus_tag="VFN42_10815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8997146.1"
                     /translation="MKLMWFHLMPYTELPDDFRDANPSVWVDIHSKLFDPRRAHHMYN
                     DFMDELEYAAQCGFDAICVNEHHSNGYGLMPSPNLIASSLARRTTDTAICVMGNSLAL
                     YNPPTRVAEEFAMIDCISGGRLIAGFPVGTPMDTCYAYGQNPSQLRQRYLEAHDLVVR
                     AWQEKDTFAFNGRFNQQRYVNIWPRPVQQPHPPIWIPGGGSVETWQWCAEMDYVYCYL
                     SYYGYKAGRATMDGFWAEMDRLGKDRNPYRAGFLQFVGVGESRQHAFDLYAEAAEYFY
                     GRCLHVDARWATPPGYTSEGSQRAGIESQVKQAADANVRGKSTGERMASVAREMNGIV
                     DNGYVIIGSPDEVAEQLREVAVNLNVGHLMLLLQFGNMSKDLTRYNTKLFAEQVMPKL
                     RDIHAGWTDHWWPQPMEAAQRAEVPAYTPKLAAE"
     gene            complement(1536..>2030)
                     /locus_tag="VFN42_10820"
     CDS             complement(1536..>2030)
                     /locus_tag="VFN42_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997147.1"
                     /translation="VVFYSLTGRTRRVAQALAAELGADIEQIDCPRFRAGFLGFWRAG
                     YASWRNRIPEIAPPAHAASDYDLVLVGGPVWAWNACTPVRAYLTRARAQLPTVAFFVT
                     VGGVGFDRALATMEALAGRRPTATLVLKDADFKQGKDRSAIAAFVAALERGKPVRSPV
                     HEPA"
BASE COUNT          331 a          651 c          674 g          374 t
ORIGIN      
        1 tatcgttgag gcctattctg cggcgagttt cggtgtgtag gcggggactt cagcccgttg
       61 ggccgcttcc atcggctgag gccaccaatg gtcggtccag ccagcatgga tgtcccgcaa
      121 ctttggcatg acctgttcgg cgaacagctt cgtgttgtac cgcgtgaggt ccttgctcat
      181 gttgccgaat tgcagcagca gcatgaggtg gccgacattc aggttcaccg cgacttcgcg
      241 caattgctcc gccacctcat ccggcgagcc gatgatgacg tagccattgt ccacgatgcc
      301 gttcatctcg cgcgccacgg aggccatgcg ttcgccggtg gacttgccgc gcacgttggc
      361 atccgccgcc tgcttcacct ggctttcgat gccggcgcgc tggctgccct cactggtgta
      421 gccgggcggc gtcgcccagc gcgcatcgac atgcaggcag cggccgtaga aatattccgc
      481 ggcttccgca tacaggtcga acgcgtgctg gcgggattcg ccgacgccga cgaattgcag
      541 gaagccggcg cggtacgggt tccggtcctt gcccaggcgg tccatctccg cccagaaccc
      601 gtccatggtg gcgcggccgg ccttgtagcc gtagtacgac agatagcaat acacgtagtc
      661 catctcggcg caccactgcc aggtctccac cgagccgccg ccggggatcc agatcggcgg
      721 atgcggctgc tgcaccgggc gcggccagat gttgacgtag cgctgctggt tgaaccggcc
      781 gttgaaggcg aaggtgtcct tctcctgcca tgcccgcacc accagatcgt gcgcctccag
      841 gtagcgctgg cgcagctggc tggggttctg gccgtaggcg tagcaggtgt ccatcggcgt
      901 gccgaccggg aatccagcga tcagtcttcc gccggaaatg cagtcgatca tggcgaattc
      961 ttccgccacg cgggtcggcg gattgtacag cgccagcgag ttgcccatga cgcagatcgc
     1021 cgtatcggtg gtgcggcgcg ccagcgacga tgcgatcagg ttgggcgagg gcatcagccc
     1081 gtagccgttg gaatgatgct cgttgacgca gatggcgtcg aagccgcact gcgccgcgta
     1141 ttccagctca tccatgaagt cgttgtacat gtgatgcgcg cggcgcggat cgaacagctt
     1201 ggagtggata tcgacccaaa ccgacgggtt cgcgtcgcgg aagtcgtccg gtagttccgt
     1261 gtacggcatc aggtggaacc acatgagctt cattggcgac ctccgttgcg cgctgctttt
     1321 ctcaccgccg ctgcgccgcc ggatcggcgc gacagcatcg acataccgat gagtatcacg
     1381 ggcacccaac tggtagtcaa ttggcgtggc ggcacgcagt ttgttcgtgc tttgcggcga
     1441 ggcaccggga accgcgcctg ccgaagcagg gccgttttcc ggcaaaccgt cctggttatc
     1501 gcgccgccgc gttcagggct ttcgccgcga ggcgttcacg caggctcatg caccgggctg
     1561 cgcaccggct tgccgcgctc gagcgccgcg acaaaagcgg cgatcgcgga tctgtccttg
     1621 ccctgcttga agtctgcatc cttcagcacg agcgtcgccg tggggcgtcg tccggccagt
     1681 gcttccatgg tggcaagcgc gcggtcgaag ccgacgccgc cgaccgtgac gaagaaggcg
     1741 acggtgggaa gctgcgccct tgcgcgcgtc aggtaggcgc ggaccggcgt gcaggcgttc
     1801 caggcccaga ccggcccgcc gaccagcaca aggtcatagt ccgacgctgc gtgcgccggt
     1861 ggggcgatct cggggatccg gttccgccaa ctggcgtatc cagcccgcca gaatccaagg
     1921 aagccggcac ggaaacgcgg gcagtcgatc tgttcgatat cggcgccgag ttccgctgca
     1981 agcgcctgtg ccacgcggcg cgtgcggccg gtgagcgaat agaaaaccac
//
LOCUS       DASSZH010000494         4022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_744754, whole genome shotgun sequence.
ACCESSION   DASSZH010000494 DASSZH010000000
VERSION     DASSZH010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744754"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1132)
                     /locus_tag="VFN42_10825"
     CDS             complement(65..1132)
                     /locus_tag="VFN42_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997148.1"
                     /translation="MTPPDSEDDDTIRVQRTVVASRRTAPPWMLPVAAIGGLCLLAAA
                     GVSLWWPRPAPHAVPAVPPAPALVAPAAPPAAAPAPPPAAPEFAVQTASQAQILAHVG
                     TGLAIFRFAPDPRIVVMDFGSLLTQGRMLDRVAALNEKAGLPHDRVLTSPQLDAAIRA
                     GGDTVATYYYGHDYSAQELVRFFALADAEHVALHPQEETLRRIMQQLGWFQPGVHAAL
                     ISIPQAGTDPRLTMAARSAILQHELSHGLYFSDPAYAAYVHRFWDSALTAEERAAFRR
                     FLGSQEYDTSLDDLMINEMQAYLMFTRDPDFFRPALIGMAPVRLAELQAAFARGMPAG
                     WLRDTLLALDTEVTQASAPIR"
     gene            complement(1173..1784)
                     /locus_tag="VFN42_10830"
     CDS             complement(1173..1784)
                     /locus_tag="VFN42_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET8997149.1"
                     /translation="MRRLIAPLLAIPLIVGAAPVFAQSNPSADQIIQSLKPKGNLLAG
                     GTRGIRLAPQAAAPAPRTYAAPMGSHQVAAAKPMAAPPASEASAPSVNLNVDFETGSA
                     NLTPQARSTLDQLGRALSSSQLAGYRFRIVGHTDTVGSPAYNKTLSEQRADAVVHYLS
                     DKFGVQPTRLEAEGVGEAGLLVPTPPQTPEPRNRRVQVINLGA"
     gene            complement(1916..3340)
                     /locus_tag="VFN42_10835"
     CDS             complement(1916..3340)
                     /locus_tag="VFN42_10835"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8997150.1"
                     /translation="MSVATSEDLLSLYDEAPLNSRYWVTFAIMAGVFLLDFFDFFLIA
                     FVMSQIGPAWKLTYGQGAVILYGAGIGAIVGAVIWGALGDRFGRKMQTVTGTLICGVA
                     AGCIGLLPTGAWVSLALLRFFVGFGLAAGVTPALTIVVEQTPTRWRTGMTSFYVVFAS
                     AGTLLASFTSAILLHAFGWRGVAMTGFVAIAIGLLVWAFVPESVRWLTCKGRFAEARE
                     QVARQLGRPLAQVPLPTVIPTAQPRASLAELYQKPSLFWQTVLIWGGSASAAYGYYLW
                     GPTIVALALKVSPAQAAGYFIYVAASGVLGKIIVSLIAPAIGRRMLGITFGFVSTVCL
                     ALAGYFNGVLVGSFPLFIILTAIAAFFVEGGFANLAPYTVEQYGVRLGARSSGLGQAA
                     NGVGKIVGPLALALLAGSSNIIAPKATAEAVFPAFALIGGLMLLVGLAYLFLGVETHG
                     RPIALGDDEPVAPPVGLGTAAGHL"
     gene            3730..>4022
                     /locus_tag="VFN42_10840"
     CDS             3730..>4022
                     /locus_tag="VFN42_10840"
                     /inference="COORDINATES: protein motif:HMM:NF013853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 family protein"
                     /protein_id="HET8997151.1"
                     /translation="MPNDLYDRDILAWSEYQAGLLRRLARGERVNDLDWEHVVEEIED
                     VGLSELHSVESYLDLILLHLLKVHAWPNSESVNRWLAEAVAFQRNARRRFAPS"
BASE COUNT          712 a         1328 c         1394 g          588 t
ORIGIN      
        1 ttcactccac aggccgccag catagctccg gcgcgccatt ctggtccatt cccggcgggc
       61 cgcgtcagcg gatcggcgcc gacgcctggg tcacctcggt atcgagcgcc aggagcgtgt
      121 cgcgcagcca tccggccggc atgccgcgcg cgaacgccgc ctgcagttcc gccagccgca
      181 ccggtgccat gccgatcagc gccggacgga agaagtccgg atcgcgggtg aacatcagat
      241 aggcctgcat ctcgttgatc atcagatcgt cgagactggt gtcgtattcc tgcgagccga
      301 ggaaccggcg gaacgctgca cgttcctccg cggtcagggc ggaatcccag aaccggtgta
      361 catacgcggc ataggccggg tcgctgaaat acaacccgtg tgacagctcg tgctgcagaa
      421 tggcgctgcg cgccgccatt gtcaggcgcg gatcggtacc cgcctgcgga atcgagatca
      481 acgccgcatg cacgccgggc tggaaccagc ccagttgctg catgatccgg cgcagggttt
      541 cctcctgcgg gtgcagcgcg acgtgctccg catccgccag ggcgaagaag cgcaccagtt
      601 cctgcgcgct gtaatcgtgg ccatagtagt aggtcgccac tgtgtcgccc ccggcacgga
      661 tcgccgcatc caactgtggg ctggtcagta cgcggtcgtg cggcagaccg gccttctcgt
      721 tcagcgcggc gacgcggtcc agcatcctgc cctgggtcag cagcgacccg aaatccatca
      781 ccacgatgcg cggatcgggc gcgaagcgga agatcgcgag tccggtgccg acatgcgcca
      841 ggatctgcgc ctgcgacgcc gtctgcaccg cgaattccgg ggcagcgggc ggcggggcag
      901 gggcggcggc cggcggggca gcgggagcca ccagtgccgg cgcgggcggc acggccggca
      961 cagcgtgcgg cgccggccgg ggccaccaca gcgacacgcc ggctgccgcc agcaggcaaa
     1021 gaccgccgat tgccgcaacc ggcagcatcc agggcggtgc agtgcgccgg gaggccacca
     1081 cggtccgctg cacacggatg gtgtcgtcgt cctccgagtc aggcggcgtc atgcgcgcgg
     1141 cgcaagcagc gagactgctg gtgtggcttc gctcacgcgc cgaggttgat cacctgcacc
     1201 cggcgattgc gcggctccgg ggtctgcggc ggcgtcggca ccagcagccc ggcttcaccc
     1261 acaccttcgg cttccagccg agtaggctgc accccgaact tgtcggagag gtagtgcacc
     1321 accgcgtcgg ctcgctgttc cgacaaggtc ttgttgtagg ccggcgagcc caccgtatcg
     1381 gtatggccaa cgatgcggaa ccggtagccg gccagttggc tgctgctcag tgcgcggccg
     1441 agctggtcca gtgtggaccg ggcctgcggc gtcagattgg cggagccggt ttcgaaatcg
     1501 acattcaggt tgaccgacgg tgcggatgcc tcgctggccg gcggcgccgc catcggcttg
     1561 gccgcggcga cctggtgcga gcccatcggc gccgcgtatg tccggggcgc cggtgccgcc
     1621 gcctgcggcg ccaggcggat tccgcgggta ccgccagcca acagattacc ttttggtttc
     1681 agcgactgaa tgatctggtc ggccgacggg ttgctttgcg cgaacaccgg ggcggcacca
     1741 acgatcagcg ggatagccaa aagcggggca atcaggcggc gcacagcatt ctcctccctc
     1801 gggaacggtg acgttctata gcacgagact atcacgtttc cagtacaaca gatggccggg
     1861 agagcggtcg atggcggagg cgggccgccc ggggcggccc gcctcctgcc ccggcctaaa
     1921 gatggccggc cgcggtcccc aatccgacgg gcggcgccac cggttcgtcg tcacccagcg
     1981 cgatgggacg gccgtgcgtc tccacgccga ggaacaggta tgccagtcca accagcagca
     2041 tcagcccacc gatgagggcg aacgccggga ataccgcttc cgccgtcgcc ttcggcgcga
     2101 tgatgttgct gctgccggcc agcagcgcca gcgccagcgg accgacgatc ttgccgacgc
     2161 cgtttgctgc ctggccaagt cccgaggagc gggcgccaag ccggacaccg tactgctcca
     2221 ccgtgtaggg tgcgagattg gcgaagccgc cctcgacgaa gaacgcggca atcgccgtca
     2281 ggatgatgaa cagcgggaag ctgccgacca gcacgccgtt gaaatacccg gccagcgcca
     2341 ggcagacggt ggagacgaaa ccgaaggtga tgccgagcat gcggcggccg atcgccgggg
     2401 cgatcagcga cacgatgatc ttgcccagca cgccggaggc ggcgacgtag atgaagtagc
     2461 cggcagcctg cgcggggctc accttcagcg ccagggcgac gatcgtcggt ccccacaggt
     2521 agtagccgta tgcggcgctg gctgaaccgc cccagatcag tacggtctgc cagaacaggc
     2581 tgggcttctg gtacaactca gccaagctgg cacgcggctg cgcggttggg atcaccgtgg
     2641 gcaacgggac ctgcgcgagc ggacggccaa gctggcgcgc gacctgctca cgggcctcgg
     2701 caaagcgccc cttgcaagtc agccagcgca cggattccgg cacgaaagcc cagaccagca
     2761 ggccgatggc gatcgcgacg aacccggtca tcgcaacgcc gcgccagccg aacgcgtgca
     2821 gcaggatcgc cgaggtgaag gaggccaaca gcgtgccggc gctggcgaac accacgtaga
     2881 agctggtcat gccggtgcgc cagcgggtcg gcgtttgctc caccacgatg gtcagcgcag
     2941 gggtgacgcc ggcggcgagg ccgaagccga cgaagaaccg cagcagtgcg agcgagaccc
     3001 aggcgccggt gggcagcagg ccgatgcagc cggccgccac gccgcagatc agcgtgccgg
     3061 tgacggtctg cattttgcgt ccgaaccggt cgcccaaggc gccccagatc acggcgccga
     3121 cgatggcgcc gataccggcc ccgtaaagga tgaccgctcc ctggccgtag gtcagcttcc
     3181 atgccgggcc gatctgcgac atgacgaagg cgatcaggaa gaagtcgaag aagtcgagca
     3241 ggaagactcc ggccatgatg gcgaaggtca cccagtagcg cgaattgagg ggtgcctcgt
     3301 catacaagga cagcaggtct tccgaggtcg cgacggacat tggtacctcc aaccggctta
     3361 tgctgaattt ttccgggccg cggctgccgg aaaccgctgg caccgttatt tcacggcggt
     3421 cctgagggag agcatcgcgt tcacacggcg gcggcgctct cgaaaggcgc ggcacgcgga
     3481 cccaatcggg ccgtcatcgg gactatacac ccggaggttg cggtgtccct atacggtcgc
     3541 gtgaaaggca ggacagttgc cggcttgcac cgggccggcg gcggatcgcc gagcaaccgc
     3601 aggggcaaaa cagatctcgg catagcgggg ggcggatttg agccagatcg cgtgccggcg
     3661 gacgcaagcg acgccctcgc gggatacggc gagatcgcgt caagcgcatt ttcgctatac
     3721 tctgtcgtca tgcccaacga tctatatgac cgcgacatcc tcgcctggtc ggaataccag
     3781 gccggcctgc tgcgccgcct cgcgcgcggc gagcgggtga acgatctcga ctgggagcat
     3841 gtggtcgagg agatcgagga cgtgggtctc tccgaactgc acagcgtcga gagctatctc
     3901 gacctgatcc tgctgcatct gctgaaagtg cacgcttggc cgaacagcga gtcggtcaac
     3961 cgctggcttg ccgaggcggt cgctttccag cggaacgctc ggcggcggtt cgcgccttcg
     4021 at
//
LOCUS       DASSZH010000495         2717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1494610, whole genome shotgun sequence.
ACCESSION   DASSZH010000495 DASSZH010000000
VERSION     DASSZH010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494610"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..108)
                     /locus_tag="VFN42_10845"
     CDS             complement(<1..108)
                     /locus_tag="VFN42_10845"
                     /inference="COORDINATES: protein motif:HMM:NF045352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997152.1"
                     /translation="MAESLQILCPACKTMNRVPLARPATAARCGNCHAPL"
     gene            complement(115..1392)
                     /locus_tag="VFN42_10850"
     CDS             complement(115..1392)
                     /locus_tag="VFN42_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8997153.1"
                     /translation="MSDVVVLGAGLGGTLMTYELLDQLRPGDRLTLIGQEPRYHFVPS
                     NPWVAVGWRKRSDVEIDLTEIMRRKQVHYITQGASRVDPANRRVELNDGSSVSYDYLV
                     IATGPDLAFDEIEGLGPRAHTQSICHIDHAAQAYEAFEQFCKHPGPVVIGAVQGASCF
                     GPAYEFAYILDTELRKRRIRSKVKMTFVTPEPYIGHLGLDGVGDTKSMMESEFRNRHI
                     HWITNAKVKQVAEGKMTVEEVDDSGAPRKTHELPFGYSMMLPAFRGVAAVRGIDKLVN
                     PRGFVQIDELQRNPAYPEIFSIGVCVAIPPVGPTPVPVGTPKTGFMIESMVTATAVNI
                     GKLLRGEEPAARATWNAICLADFGDGGVAFVAQPQIPPRNVNWSAKGAWVHMAKVAFE
                     KYFLRKMRAGQSEHFFESFVLRRLNLHKLHAAE"
     gene            1495..1872
                     /locus_tag="VFN42_10855"
     CDS             1495..1872
                     /locus_tag="VFN42_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET8997154.1"
                     /translation="MILDPEQMAAAADEASELLKSLANRHRLLILCQLTEGERSVGDL
                     AAFLHLRDSTVSQHLALLRREGLVQARRDGQTIWYAIASLPARQVLETLFEVFCKPSS
                     ICAPPQRPRAAAPSTTRGSGRKR"
     gene            2013..2372
                     /locus_tag="VFN42_10860"
     CDS             2013..2372
                     /locus_tag="VFN42_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET8997155.1"
                     /translation="MTARPHLQELTPTQVHDALREHSIILVDVREPQEYITERIHGAL
                     LFPLSTFDPLALPTGDRPVVLHCGSGKRSAVAVGRCMEAGVPVTIHLKGGILAWKHAR
                     FPTVSLDPSTGAVVDRK"
     gene            2422..>2717
                     /locus_tag="VFN42_10865"
     CDS             2422..>2717
                     /locus_tag="VFN42_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HET8997156.1"
                     /translation="MRIPIITVWLVLCAAAAAHAQATFTVHRAPLTDEKAVFATVESP
                     NVVPARARIGGTVASLSVRQGDAVKQGQVIAVVGDEKLILQINSLDAQIAGLQS"
BASE COUNT          443 a          950 c          868 g          456 t
ORIGIN      
        1 caacggcgcg tgacaattgc cgcaacgcgc cgccgtggcc ggacgcgcca gcggcacgcg
       61 gttcatggtc ttgcaggccg gacacaggat ttgcaacgat tcggccatgc cccctcactc
      121 cgccgcgtgc agcttgtgca ggttcagccg gcgcagcacg aagctttcga agaagtgctc
      181 gctctgcccc gcgcgcatct tgcgcaggaa gtatttttcg aacgccacct tggccatgtg
      241 cacccaggcg cctttggcgg accagttgac attgcgcggc gggatctgcg gctgcgccac
      301 gaaggccacg ccgccatcgc cgaaatccgc caggcagatc gcgttccagg tggcacgcgc
      361 cgccggctcc tcaccacgca gcagcttgcc gatgttcacc gcggtggcgg tcaccatcga
      421 ctcgatcatg aacccggtct tcggcgtgcc gaccggcacc ggcgtcggcc ccaccggcgg
      481 gatggcgacg cacacgccga tgctgaagat ctccggatac gccggattgc gctgcaactc
      541 gtcgatctgc acgaagccgc gcgggttcac cagcttatcg atgccgcgta ccgccgccac
      601 gccacggaac gccggcagca tcatggaata gccgaacggc agctcgtgcg tcttgcgcgg
      661 tgcgccgctg tcatccacct cctcgaccgt catcttgcct tcggcgacct gcttcacctt
      721 ggcgttggtg atccagtgga tgtggcggtt gcggaattcg ctctccatca tcgacttggt
      781 gtcgccgacg ccgtcgaggc cgagatggcc gatatagggt tccggcgtca cgaaggtcat
      841 cttgaccttg ctgcggatcc gccgtttgcg cagctcggtg tccaggatat aggcgaattc
      901 ataggccggc ccgaagcagg acgcgccctg caccgcgccg atcaccaccg gcccgggatg
      961 cttgcagaac tgctcgaacg cttcatacgc ctgcgccgcg tggtcgatgt ggcagatcga
     1021 ctgggtgtgc gcccgcgggc ccagcccctc gatctcgtcg aacgccaggt cgggaccggt
     1081 ggcgatcacc aggtagtcgt agctcacgct gctgccgtcg ttcagctcta ccctgcggtt
     1141 cgccggatcg acgcggctgg cgccttgcgt gatgtagtgc acctgtttgc ggcgcatgat
     1201 ctcggtcagg tcgatctcga catccgaccg cttgcgccag ccgacggcga cccagggatt
     1261 ggacggcacg aaatgatagc gcggctcctg gccgatcagg gtcagccggt cgcccggccg
     1321 aagctggtcc agcagctcat aggtcatcag ggttccgccc aggccggcgc cgagcacaac
     1381 gacgtcgctc attgttctgt ctcctggcac gctccgcgcg cgcccccggt gggattgctg
     1441 gcttgacaaa tattcgcatc atcgtagata cgcaagtgtg aaagtaagaa accgatgatc
     1501 ctcgaccctg agcagatggc cgccgccgcc gacgaggcga gcgagctgct gaaatccctg
     1561 gccaaccgcc accggctgct gatcctgtgc cagcttaccg aaggcgagcg ctcggtcggc
     1621 gacctggccg cgttcctgca tttgcgcgat tccaccgtgt cgcagcattt ggcgctgctg
     1681 cggcgcgagg gcctcgtgca ggcgcgccgg gacggccaga ccatctggta cgccatcgcc
     1741 agcctgcctg cccggcaggt gctggagacg ctgttcgagg tcttctgcaa gccgagttcc
     1801 atctgtgccc cgcctcaacg gccgcgggcc gcggcgccgt ccaccacacg cggttcggga
     1861 cgcaagcgct gatgacggct ggctccatcg caattctcgt ccgccgcgcg cggcgcgcat
     1921 tgacacagat caatgtcgga aacggcgcga agacggttaa tgcgccgtcg gggacggcag
     1981 cgcctgcttc tcacacgacc caggaggcac ccatgaccgc caggccccac ttgcaggaac
     2041 tcacgcccac ccaggtgcac gatgcgctgc gcgagcacag catcatcctg gtcgatgtgc
     2101 gcgagccgca ggaatacatc accgagcgca tccacggtgc gctgctgttc ccgctgtcca
     2161 ccttcgatcc gctggcgctg cccaccggcg accggccggt ggtgctgcat tgcggctccg
     2221 gcaagcgctc cgccgtcgcg gtggggcgct gcatggaagc cggcgtgccg gtcaccatcc
     2281 acctgaaggg cggcatcctg gcgtggaagc acgcgcgctt ccccaccgtc agcctggacc
     2341 ccagcaccgg cgccgtggtg gaccggaaat agcccgaccc ggacgccgcc accgcgcggc
     2401 aggccggccg agaggaccac aatgcgaatc ccgatcatca ccgtctggct cgtgctctgc
     2461 gcggcagcag ccgcccatgc ccaggcgaca ttcaccgtgc accgtgcgcc gctgaccgac
     2521 gagaaggcgg tgttcgccac ggtggaaagt cccaacgtcg ttcccgcccg cgcccgcatc
     2581 ggcggcacgg ttgccagcct gtcggtccgc cagggcgacg cggtgaagca gggtcaggtg
     2641 attgcggtgg tcggcgacga gaagctgatc ctgcagatca actcgctgga tgcgcagatc
     2701 gccgggctgc aatcgca
//
LOCUS       DASSZH010000496         3662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1136907, whole genome shotgun sequence.
ACCESSION   DASSZH010000496 DASSZH010000000
VERSION     DASSZH010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136907"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1933
                     /locus_tag="VFN42_10870"
     CDS             <1..1933
                     /locus_tag="VFN42_10870"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997157.1"
                     /translation="GGLLLARDASGEARELLLRCCGIAPGRAEAWHALGLALAATGDT
                     ALAHTAFIEAQRLEPSAPEHALRGIEAAQAAGDAEPELARLEALGREDPFNPVWPLAR
                     GMLLECLGNRSAAIDALEAAAVLAPDAPLPAALLAGVLARSQQHREANAALQRAIALD
                     PENTDLSNMRAVVLMRMQRHAEALALLLELQQRCGDSAALLSNLANASVYLGLHDAAI
                     DSLRRAMAMDPEAVLPRRVLCNVLPYCDGTTGVQLLAALRNCAECAPCEAPAVLGNLP
                     DPDRKLTVGLLSGSLKTHPVAWLTLAGFETLDPAQFALVCLVQNPKPHDLMARRFRVI
                     AREWIETDALNDAALAQTARDAGVDVLIDLGGYGEGSRMQACRHRLAPVQIKWVGMQS
                     HSSGLPEMDWMLTDRWETPPGSEHLYSEHLLRLPDGYVCYTPPPYAPDIVTLPAMANG
                     HVTFGCFNNLAKITTRVVAVWAAILHRVPGARMVLKTHQFVDPPTCDRIRDAFVQHGI
                     TADRIDLRGPSRHRAFLGEYNGIDIVLDPFPYSGGLTTCEALWMGVPTITMPGDFFAA
                     RHSFSHLSNAGLAGWAADDLASYIELAVAKAADLDALAALRAGLRARVKASPLCDAPR
                     FGRNLGAALRHAWQTWCWQ"
     gene            1930..2634
                     /locus_tag="VFN42_10875"
     CDS             1930..2634
                     /locus_tag="VFN42_10875"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET8997158.1"
                     /translation="MSGFASHAYWEQRYRAGGDSGAGSGGVLLHYKAAVINAFIADNH
                     VASVLDLGCGDGRLLSLLRAPSYVGVDVSPTVLSGCASRFPQHRFLGLDALDQEPAAE
                     LALSIDVLFHLVEDQVFIRSMDTLFAHATRFVLIYASNFVCDWPAPHVRHRRFTDHVV
                     RAWPDWRLLAHLPNPHPYHPARPDETSFADFFVYGRPSAGCAIRVPGRSAQHAGDERG
                     QPLIVADFVVGEGGNP"
     gene            complement(2554..>3662)
                     /locus_tag="VFN42_10880"
     CDS             complement(2554..>3662)
                     /locus_tag="VFN42_10880"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET8997159.1"
                     /translation="DWEFLSVNHGSFGATPLVVLAAQADWRRRMEAQTGRFFGQVLPN
                     ALRHAADRLGAFIGAEGKDIAFVDNATTGCNAVLRSLRLQAGEEVVVLTHGYGAVRNT
                     VRFVTERAGARMVEAAIPFPRPDPDAVVAAVQAVLTSHTRLALLDHITSGRALVLPVQ
                     RMVDACHAAGVPVLVDGAHAPAQVDLDLRALGADWYAGNCHKWLCAPKGASFLWAAPH
                     RQHDLHPVTISHGFGQGFLHEFDWTGTRDPSPYLCVDTAIDFHARLGGGALRARNVAL
                     AAEATALLAGRLNTEAGGTSQMAGSMGVVRLPLPGSATAGHAGNLRQRLMAAGSDAPV
                     HAIDGSLWLRISAFAYNEIGDYERLAALVAGVLR"
BASE COUNT          540 a         1240 c         1258 g          624 t
ORIGIN      
        1 gggcggcctg ctgctggccc gcgacgcctc tggcgaggcg cgggagctgc tgttgcgctg
       61 ctgcggcatc gcccccggcc gggccgaagc ctggcatgcg ctgggactcg ccttggcagc
      121 gaccggcgac accgcgctgg cgcataccgc gttcatcgag gcgcagcgcc tggagcccag
      181 cgcgcctgag catgctctgc gcgggatcga ggccgcgcag gcggccggcg atgccgaacc
      241 cgaacttgcc cgcctggaag ctctgggacg ggaggatccg ttcaatccgg tgtggccgct
      301 ggcgcgcggc atgttgctgg agtgcttggg aaaccgcagc gccgcaatcg atgccctgga
      361 ggcggccgct gtcctggcgc cggacgcgcc tttgcccgcc gccctgctcg ccggtgtgct
      421 ggcccgctcg cagcagcacc gggaggccaa cgccgccttg cagcgcgcca tcgcactcga
      481 tcccgagaac accgacctgt ccaatatgcg ggccgtggtg ctgatgcgca tgcagcggca
      541 tgcagaagcg ctcgccttgt tgttggagct gcagcagcgc tgcggcgaca gcgctgcgct
      601 gctcagcaat ctggccaatg cttccgtgta tctcggcctg cacgatgcgg cgattgattc
      661 gctccgccgc gccatggcga tggatcccga agcggtgctg ccccggcggg tgctgtgcaa
      721 cgtcctgccg tattgtgacg gcaccaccgg cgtacagttg cttgcggcac tgcgcaactg
      781 cgccgaatgc gcgccgtgtg aggcgccggc ggtcctcggc aacctgcccg atccggaccg
      841 caagctgacg gtcgggctgc tctccggttc gctgaagacg catcctgtcg cctggctgac
      901 cctcgccgga ttcgagacgc tcgatccggc gcagtttgcg ctggtatgct tggtgcagaa
      961 ccccaaaccg cacgacctga tggcccgccg cttccgtgtg atcgcgcgcg agtggatcga
     1021 gacggacgca ctgaatgacg cggcgctggc gcagacggcg cgggatgccg gcgtcgacgt
     1081 gttgatcgac ctgggcggat acggcgaggg ctcgcgcatg caggcctgcc gtcatcggct
     1141 ggcgccggtg cagatcaagt gggtgggaat gcaaagccac agcagtggcc tgccggaaat
     1201 ggactggatg ctgaccgatc gctgggagac accgcccggg tcggagcacc tgtattccga
     1261 gcatctgctg cgcctgcccg atggctatgt ctgctacacc ccgccgccct atgcgcccga
     1321 tatcgtcacc ttgccggcca tggcgaacgg tcatgtgacc ttcggctgct tcaacaacct
     1381 ggcaaagatc acgacccggg tggtggctgt ctgggcggca atcctgcacc gtgtgcccgg
     1441 tgcccggatg gtgctgaaga cgcaccagtt cgtcgatccg ccaacctgcg accgcatccg
     1501 tgacgcgttc gtgcaacacg ggatcaccgc cgatcgcatc gacctgcgcg gcccgtcgcg
     1561 gcatcgggcg ttcctgggcg agtacaacgg gatcgatatc gtgctggatc cattccccta
     1621 ctcgggcgga cttaccacct gcgaagcgct ttggatgggt gtgcccacca ttaccatgcc
     1681 cggcgatttc ttcgccgcac gccactcgtt cagtcacctc agcaatgccg ggctggccgg
     1741 atgggctgcc gatgacctgg cgtcctacat cgagctggcg gtggcgaagg ctgctgacct
     1801 cgatgccttg gccgcgcttc gcgccggcct gcgtgcgcgg gtgaaggcga gtccgctgtg
     1861 tgatgcaccg cgtttcggcc gcaacctggg tgcggcgctg cggcacgcgt ggcaaacctg
     1921 gtgctggcaa tgagcggctt cgcctcgcac gcgtactggg agcaacgcta tcgcgcgggc
     1981 ggcgattccg gcgcgggctc cggcggcgtc ctgctgcact ataaggcggc ggtgatcaac
     2041 gccttcatcg ccgacaacca tgtggccagc gtgctggatc ttggatgcgg cgacgggcgg
     2101 ctactgtcgc tgctgcgtgc accgtcctat gtcggcgtgg atgtctcgcc aaccgtgctg
     2161 tccggttgcg ccagccgctt tccgcaacat cgcttcctcg gcctcgacgc gctggatcag
     2221 gagccggcag cggagctggc cttgtcgatc gatgtcctgt tccatctggt ggaggaccag
     2281 gtgttcatcc gctcgatgga cacgctgttc gcccatgcga cgcgctttgt gctgatctat
     2341 gccagcaatt tcgtgtgtga ttggccggcg ccccatgtgc gccaccggcg cttcaccgac
     2401 catgtggtgc gcgcctggcc ggattggcgg ctgctggctc acctgccgaa cccgcacccg
     2461 tatcatccgg cgcgtccgga cgagacgtcg tttgccgatt ttttcgtgta tggcaggccg
     2521 tccgccggat gtgcgatccg ggttccgggc cgctcagcgc agcacgccgg cgacgagcgc
     2581 ggccagccgc tcatagtcgc cgatttcgtt gtaggcgaag gcggaaatcc gtagccaaag
     2641 gctgccgtcg atcgcgtgca ccggcgcatc gctacccgcc gccatcaggc gttgccgcaa
     2701 attgccagcg tggccggcgg ttgcgctccc gggaagcggc agccgcacca cgcccatcga
     2761 ccccgccatc tgggaagtgc cgcccgcttc ggtgttcagc cggccggcaa gcagggcggt
     2821 tgcctcggcg gcgagcgcga cattgcgggc ccgcagcgcg ccgccaccaa gccgggcatg
     2881 gaaatcgatg gctgtgtcca cgcagagata ggggctggga tcgcgcgtgc cggtccagtc
     2941 gaattcgtgg aggaagccct ggccgaaccc gtgggagatg gtgacgggat gcaggtcgtg
     3001 ctggcggtgc ggtgctgccc agaggaacga cgccccctta ggcgcgcaca gccatttatg
     3061 gcagttgccg gcataccagt cggcacccag cgcgcgcaga tcgagatcga cctgcgcggg
     3121 cgcgtgggca ccgtcgacca gcaccggcac gccggcggcg tggcaggcat ccaccatgcg
     3181 ttgcaccggc agcaccagtg cgcggccgga ggtgatatgg tccagcagcg ccagccgcgt
     3241 gtgcgaagtc agcacggcct gcactgcggc gaccaccgca tccggatcag gcctgggaaa
     3301 cgggatggcg gcttccacca tgcgcgcgcc ggcgcgctcg gtgacgaagc gcacggtatt
     3361 gcgcaccgcg ccgtaaccgt gcgtcagcac caccacctcc tcgccggctt gcagccgcag
     3421 gctgcgcagc actgcgttgc agccggtggt ggcgttgtcc acgaaggcaa tgtccttgcc
     3481 ctcggcacct atgaacgcgc ccagccggtc ggcggcgtgc cgcagcgcgt ttggcagcac
     3541 ctggccgaag aagcgtccgg tctgcgcttc catgcgccgg cgccagtcgg cctgcgcggc
     3601 cagcaccaca agcggggtgg caccgaagga gccgtgattg acgctgagga attcccagtc
     3661 ca
//
LOCUS       DASSZH010000497         2600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_727860, whole genome shotgun sequence.
ACCESSION   DASSZH010000497 DASSZH010000000
VERSION     DASSZH010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727860"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(320..1180)
                     /gene="hpnD"
                     /locus_tag="VFN42_10885"
     CDS             complement(320..1180)
                     /gene="hpnD"
                     /locus_tag="VFN42_10885"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HET8997160.1"
                     /translation="MAAATPLGVDQADLDAVEAIVRAAGTSFYRGMRVLPPDRRHAMY
                     AIYAFCRLVDDIADDEAPLADRLPRLAAWRDHIDGLYRGAAEEPVTRVLRAAVQRFDL
                     RREDFVAVIDGMQMDAEQVIVAPDLATLDLYCDRVASAVGRLSVRAFGDASPAADRVA
                     HALGRALQLTNILRDVAEDAERGRLYLPSEYLDEAGVPHDAKAAVRAPGLPAVCARLA
                     SLAHGYFRAAEQAMQACDARAMKPARMMGATYAAILSRLERSGWTHPEQRVSLPKWQK
                     LWLALRFGLG"
     gene            complement(1180..2028)
                     /gene="hpnC"
                     /locus_tag="VFN42_10890"
     CDS             complement(1180..2028)
                     /gene="hpnC"
                     /locus_tag="VFN42_10890"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010513723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HET8997161.1"
                     /translation="MDATVDEIANVEAWSGKDRGDENFPVGSWLIRADLRTHVHAFYA
                     FARNADDIADSPTLTPEDKIARLDIMEEVLLGRRDTGSPSALRLRESLAQTGVTPKHA
                     AELLIAFRQDAVKRRYATLTELYDYCRYSAVPVGRHVLDLHGEPHKTHAPSDALCTSL
                     QVLNHIQDCVQDYKRLDRCYLPQRMLEDANASPEHLLRPAASPGVRRVFDSLLHRVDR
                     MNHFALDLPKRARDRRLRVETAAILSLSKRLAARLQREDPVRGRVALSKADKVGSLLV
                     ALRFLL"
     gene            complement(2028..>2600)
                     /locus_tag="VFN42_10895"
     CDS             complement(2028..>2600)
                     /locus_tag="VFN42_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8997162.1"
                     /translation="LYPFARVNDPLSATAAGAGGTVLIRRTALRRIGGIAAVRGALID
                     DVALAGAVKRGGRIWLGHSALARSERPYPGFPDVWQMIARTAYVQLRYSPLLLLLTVL
                     AMTLVYLVPPFAALFAPGLPRWLGLAAWAMMASCYLPTLRRFGRSPLWSLAMPAIAAF
                     YLAATLGSAVNHYRGRGVAWKGRAYQGTGA"
BASE COUNT          472 a          928 c          855 g          345 t
ORIGIN      
        1 gctcccgcgc tgggagcccc cgccagcaag ccgttggtgg ccggcctgcg ccggccatga
       61 caccgagcgg ttacccatga caccgagcgg ttacccatga caccgagcga ttacccacga
      121 cgccgagggt tacccacgac gccgagggtc cccacgacgc cgagggtccc cacgacgccg
      181 agggtcccca cgacgccgag ggtccccacg atataccgag gggtcaccca cgatacaccg
      241 aggggtcacc cacgacaccg agcgatcacc cacgactgag gtcgccactg acacggagct
      301 caaccctgac accgaacggt caccccaatc cgaagcgcag cgccagccac agcttctgcc
      361 acttgggcag gctgacgcgc tgctcgggat gtgtccagcc tgagcgctcc agccgcgaca
      421 ggatcgccgc gtaggtcgcg cccatcatgc gggccggctt catcgcccgc gcatcgcagg
      481 cctgcatcgc ctgctccgcc gcgcggaaat agccgtgcgc cagggaagcc agacgcgcgc
      541 acaccgccgg caatccgggc gcgcgcaccg ccgcctttgc gtcgtgcgga acacccgcct
      601 catccagata ttcgctgggt agatacagcc gaccgcgttc cgcatcctcc gcgacgtcac
      661 gcaggatgtt ggtcagctgc aaggcgcgcc ccagcgcatg cgccacgcgg tcggccgccg
      721 gcgaggcatc gccgaaggcg cgcaccgaca gccgcccgac cgctgaggca acgcggtcgc
      781 agtacagatc gagagtcgcc agatcgggcg cgacgatcac ctgctccgcg tccatttgca
      841 tgccgtcgat gaccgccacg aaatcctcgc ggcgcaaatc gaaacgctgc acggcggcgc
      901 gcaggacgcg cgtcaccggc tcctctgcgg cgccgcgata caggccgtcg atgtggtccc
      961 gccatgcggc aagccgcggc aaccggtccg ccagcggcgc ttcatcgtcg gcaatgtcgt
     1021 ccaccaggcg gcagaacgca tagatggcgt acattgcgtg ccggcggtcc ggcggcagca
     1081 cgcgcatgcc gcgatagaag gatgtgccgg cggcgcgcac gatcgcctcg acggcgtcga
     1141 gatcggcctg atcgacgccg agcggagtgg cggcggccat cagagcagga aacgcaaggc
     1201 gacaagcagg ctgccgacct tatcagcctt ggacaaggcc acgcgcccgc gcaccgggtc
     1261 ctcccgttgc aaccgtgcgg caagccgctt ggacaggctc aggatcgccg cggtttccac
     1321 ccgcaaccgc cggtccctgg cgcgcttcgg caggtccaag gcgaagtggt tcatccgatc
     1381 gacgcgatgc agcagggaat cgaacacccg gcgcacccca ggactggctg ccgggcgcag
     1441 caggtgttcg ggcgatgcgt tggcatcctc caacatgcgc tgcggcagat agcagcggtc
     1501 caaccgtttg tagtcctgca cgcaatcctg gatatgattc agcacttgca gcgaggtgca
     1561 caaggcatcg gacggcgcgt gcgtcttgtg cggctcgccg tgcagatcca gcacgtggcg
     1621 gccgaccggc acggcggaat agcggcaata gtcgtaaagc tcggtcagcg tcgcatagcg
     1681 ccgtttgacc gcatcctgcc ggaacgcgat cagcagttcg gcggcatgtt tcggcgtgac
     1741 cccggtctgc gccaggctct cccgcagccg cagcgcgctg ggggaaccag tatcacgccg
     1801 gcccagcagc acctcctcca tgatgtcgag gcgcgcgatc ttgtcttccg gagtcagcgt
     1861 gggggaatcg gcgatatcgt cggcattgcg ggcaaaggcg tagaacgcgt gcacatgcgt
     1921 ccgcaggtcg gcacggatca gccacgaccc gaccgggaaa ttctcgtcgc cgcggtcctt
     1981 gcccgaccag gcctcgacat tggcgatctc gtcgaccgtc gcatccatca ggcgcccgtc
     2041 ccctggtacg ctcgtccctt ccatgccacg ccacggccgc gatagtggtt caccgcggag
     2101 ccgagggtcg ccgccaggta gaaggcggcg atggccggca tggccaggct ccataacggt
     2161 gaccgcccga aacggcgtag cgtgggcaga tagcaggacg ccatcatggc ccaggcagcc
     2221 agcccaagcc aacggggtag gccgggcgcg aacagcgccg cgaagggcgg caccagatag
     2281 accagcgtca tcgccagcac ggtcagcagc agcagcagcg gcgaatagcg caactggacg
     2341 taggcggtgc gggcgatcat ctgccagaca tcggggaatc cggggtaggg gcgttccgac
     2401 cgcgccaagg cggaatggcc cagccagatg cggccgccgc gcttcaccgc gccggcgagc
     2461 gccacatcat cgatcagtgc gccccgcacc gcggcaatgc cgccgatgcg ccgcagcgct
     2521 gtgcggcgga tcagcacggt cccgcccgcc ccggcggccg tggcgctcaa cggatcgttg
     2581 acccgggcaa acggatacag
//
LOCUS       DASSZH010000498         2984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_710769, whole genome shotgun sequence.
ACCESSION   DASSZH010000498 DASSZH010000000
VERSION     DASSZH010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710769"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VFN42_10900"
     CDS             <1..547
                     /locus_tag="VFN42_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015072449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HET8997163.1"
                     /translation="TALIAVLSLAPSLLVMVTAFTRIIIVLSLLRSALSTQGAPPNMV
                     LIGLAPMAAGKVPEMEGMRLAAAPFHRFMTDNLRPSDLQFFLDLGHMAPSAAADQAPW
                     RAVVPAFIVGELRRAFEMGFLLFLPFLVIDMVIASVLMSLGMMMLPPSVVSLPFKLIF
                     FVMVDGWRLVAGSLVQSFGGS"
     gene            623..2077
                     /gene="glgA"
                     /locus_tag="VFN42_10905"
     CDS             623..2077
                     /gene="glgA"
                     /locus_tag="VFN42_10905"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HET8997164.1"
                     /translation="MRVLSVASELYPLVKTGGLADVAGALPLALHRLGMTVRSLIPGY
                     PAVMHRLDDAQPVHVFDHLFGGPARLLAATAGTGLDLLVLDAPHLYDRPGNPYRQDAG
                     GDWPDNPQRFAALSRAAADIGLGCLPSFVPDIVHAHDWQAGLAPAYLRYAGGPRPGTV
                     MTVHNLAFQGGCPPWLLGELGLPPYAYAVDGVEYYGGIGFLKAGLWAADRITTVSPSY
                     ALEIQTDAGGMGLGGLLRSRAGVLSGILNGIDESVWNPATDPHLAATYGPDTAATARA
                     ANKAAVQQRYGLPVESDRMLFGVVSRLEWQKGLDLLLECLPGLLQTGAQLVMLGSGDP
                     GLQSGFLAAHQAHPDRIGVTIGYDESLAHQIQAGSDAILVPSRFEPCGLTQLCALHYG
                     AVPIVARSGGLADTVIDANEAALATGVATGLQFSPGSSEQLLAAIRRAMGLWQNRALW
                     QQLQQNGMAAPVGWNGPARRYATLFQQLVEERRQ"
     gene            2074..>2984
                     /locus_tag="VFN42_10910"
     CDS             2074..>2984
                     /locus_tag="VFN42_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3445 domain-containing protein"
                     /protein_id="HET8997165.1"
                     /translation="MTDATRAELPPETVHLSFEPGPYRMAMGLITLPESAWFELDDRY
                     PDEMAQRRQLLDTQRDAVFAALPRAAEACRETLAEVAANLATHYSAWFGRDGDQLHNR
                     LTGETWNLAQPEYHPLELAGRLVQEDLCLIQLEDSGPVLTAAALCFPTRWLLAEKLGK
                     PLADVHERVPFYADKLARPVDRFMQHVKPGYVAARLNWSVIDNPALFQPSGKWRRTHN
                     DTITAANAGEALFLRVERQTLRRLPASGAVLFGIRVHSYPLARAIVTPRLAAELADAV
                     RALPEPTAHYKSILSIRDALLGWLDARA"
BASE COUNT          420 a         1047 c         1029 g          488 t
ORIGIN      
        1 caccgcgttg atcgcggtgc tgtcgctggc gcccagcctg ctggtgatgg tcaccgcctt
       61 cacccgcatc atcatcgtcc tgtcgctgct gcgcagcgcg ctcagcaccc agggcgcgcc
      121 gcccaacatg gtgctgatcg ggctggcgcc gatggcggcg ggcaaggtac cggagatgga
      181 aggcatgcgc ctggccgccg cgccgttcca ccgcttcatg acggacaatc tgcgtccgtc
      241 cgatctgcag ttcttcctcg accttggcca catggcgccc tccgccgcag cggaccaggc
      301 gccgtggcgt gcggtggtgc cggccttcat cgtgggcgag ctgcgccgcg ccttcgagat
      361 ggggttcctg ctgttcctgc ctttcctggt gatcgacatg gtgatcgcca gcgtgctgat
      421 gagcctgggc atgatgatgc tgccgcccag cgtggtgtcg ctgccgttca agctgatctt
      481 cttcgtcatg gtggatggct ggcgactggt ggctggcagc ctggtgcaga gcttcggcgg
      541 ttcgtagcgg gccacccctc ccgcaaccgg cgccgctggt gtacggcagt ccggcaagcc
      601 gaatgaagcc gcccgcatgc cgttgcgcgt cctgtccgtt gcctccgaac tgtatccgct
      661 ggtgaagacc ggcgggcttg ccgacgtggc gggcgccttg ccgctcgcgc tgcatcggct
      721 agggatgacg gtgcgcagcc tgatccccgg ctatcccgcc gtgatgcacc ggctggacga
      781 cgcccagccc gtgcatgtgt tcgatcacct gttcggcggc ccggcccggt tgctggcggc
      841 gaccgccggc accgggctcg acctgctggt gctggacgcg ccgcatctct acgaccggcc
      901 cggcaatccc taccggcagg atgccggcgg ggactggccg gacaacccgc aacgcttcgc
      961 ggcgctgagc cgggcggcag ccgatatcgg tctcggctgc ctgccgtcct tcgtccccga
     1021 cattgtgcat gcgcatgact ggcaggccgg tctggcgccc gcctacctgc gctatgcggg
     1081 cgggccgcgg cccggcacgg tgatgaccgt gcacaacctg gccttccagg gcggttgccc
     1141 gccctggctc ctgggcgaac tcggcttgcc gccgtacgct tatgcggtgg acggcgtcga
     1201 gtactacggt ggcatcggct tcctgaaggc cggactgtgg gcggccgacc ggatcaccac
     1261 ggtgtcgccg agctatgcgc tggagatcca gacggatgca ggcggaatgg ggctgggcgg
     1321 gctgctgcgg tcccgcgccg gcgtgctgtc cggcatcctg aacggcatcg acgagtcggt
     1381 atggaacccg gccaccgatc cgcaccttgc cgccacctac ggcccggaca ccgccgctac
     1441 ggcgcgggcc gccaacaagg ccgcggtgca acagcgctac ggcctgccgg tcgaatcgga
     1501 ccgcatgctg ttcggggtgg tcagccggct ggagtggcag aagggtctcg atctgctgct
     1561 ggagtgcctg ccgggcctgc tgcagaccgg cgcgcaattg gtgatgctcg gctccggcga
     1621 ccccgggctg caaagcggct tccttgccgc gcaccaggca catccggacc ggatcggcgt
     1681 caccattggc tatgacgagt cgctggcaca ccagatccag gccggcagcg acgccatcct
     1741 ggtgccgtcg cgcttcgaac cctgcggcct gacccagctc tgcgccctgc actacggcgc
     1801 ggtgccgatc gtggcgcggt cggggggact ggccgacacg gtgatcgacg ccaatgaggc
     1861 ggcgctcgcc accggcgtcg ccaccggcct gcaattctcc ccgggcagca gcgagcagtt
     1921 gctggccgcg attcgacgcg cgatgggcct ctggcagaac cgggcgctgt ggcagcagct
     1981 gcagcagaac gggatggcgg cgccggtcgg ctggaatggc ccggcgcgcc gctacgccac
     2041 gctgttccag caactggtcg aggaacgacg gcaatgaccg atgcgacgcg cgccgaactg
     2101 ccgccggaga cagttcacct gtccttcgaa cccggcccgt atcgcatggc catgggcctg
     2161 atcaccctgc cggaatcggc gtggttcgaa ctggatgacc gctatccgga cgagatggcg
     2221 cagcgccgcc agttgctgga cacgcagcgc gacgcggtgt tcgccgcact gccccgcgcc
     2281 gccgaggcct gccgcgaaac gctggcggaa gtcgccgcca atctcgcaac gcattactcc
     2341 gcctggttcg gtcgcgacgg tgaccaattg cacaaccggc tgaccggtga gacctggaac
     2401 ctcgcgcaac ccgaatacca cccgctcgaa ctcgccggcc ggctggtgca ggaggatctt
     2461 tgcctgatcc agctcgagga ttccgggccg gtgctcaccg cggcggcgct gtgctttccc
     2521 acccgctggc tcctggcgga gaagctcggc aagccgctcg cggacgtgca tgaaagggtg
     2581 ccgttctacg ccgacaagct ggcccgcccg gtcgaccgct tcatgcagca cgtgaagccc
     2641 ggctatgtcg cggcccggct gaactggtcg gtgatcgaca acccggccct gttccagccg
     2701 tccggcaaat ggcggcgcac gcacaacgac accatcaccg ccgccaatgc cggggaagcg
     2761 ctattcctgc gggtggagcg gcagacgctg cgacgcttgc cggcgagcgg cgcggtgctg
     2821 ttcggcatcc gcgtgcacag ctacccactg gcgcgggcca tcgtcacacc caggctggcg
     2881 gcggagttgg ccgacgcggt gcgtgcgctg ccggagccta ccgcgcacta caagagcatc
     2941 ctgtcgatcc gcgacgccct gctgggctgg ctggacgcgc gggc
//
LOCUS       DASSZH010000499         4095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1409292, whole genome shotgun sequence.
ACCESSION   DASSZH010000499 DASSZH010000000
VERSION     DASSZH010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409292"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1438)
                     /locus_tag="VFN42_10915"
     CDS             complement(<1..1438)
                     /locus_tag="VFN42_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET8997166.1"
                     /translation="MISAVFVDRPRLAIVLAILITMAGVLSLFRIPVAQFPDIVPPQI
                     IVSGSFPGASAAVVESTVAQPLESAVVGVDKMIYMKSNSGNDGSYSLSVFFALGSNPD
                     IDLVNVNNRVQQALSKLPPEVQRAGVTLRKRSAAILEFLQFYSEGGKQDPLFISNYVT
                     INVLDRLARTPGVGDAFLFGKMDYAMRVWFDMNRLVALHLAPSDIIKAIQAQNVQASV
                     GRVGARPTPDSTQFQLNVQTQGRLTTPKQFGNIVIRANPDGSVLRLHDVAQVELGAAS
                     MDTESRLNGNPAVSIGLYLAPGANAVATSARVSRVLRELSKRYPPGLRSRVFYDSSGF
                     VAATIDEVVKTLAIAFGLVVLVVFLFLGNVRATIIPIVAVPVSLIGTFAVLLAIGYSA
                     NTISLLAMVLGIGVVVDDAIVVVENAERVMAEEPGLGPKEATKKAMRQITGPVIAISL
                     VLLSVFVPVAFIPGVSGELFRQFAVTISV"
     gene            complement(1443..2606)
                     /locus_tag="VFN42_10920"
     CDS             complement(1443..2606)
                     /locus_tag="VFN42_10920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET8997167.1"
                     /translation="MSLRLPFGLLAGMLLSCVAAQAQMPSGPPAVGVARATQTAITET
                     SEYVGRVQAIDRVALVPRVTAFLDQRLFTEGTEVAAGQLLFRLERGPFEAAVQQQEAA
                     VAEASARLVNANIQLARAQQLLHTPAGQRSSVDDAVANQRAAAAQVLAAQAQLQVARI
                     NLGYTEIHAPIAGKISRSTVSTGNVVSPGSGALATIVSQDPIYVLFPIAVRDEVDLEK
                     RYAGKGGLNAVVVKLRLPDGTLYKPEGKIDYVEPSVQQNTDTIVLRASIANPPRRPAQ
                     PGQPVDRPLIDGEFVTVLVEGVQPVTALGIPRAAVLSDQQGDYVFVVSPSNQIEQRRI
                     QLGQSTPTTAVVVSGLKQGEMVVVNGIQRVRPGITVTPGPASTPPGGPPAAGH"
     gene            2835..4004
                     /locus_tag="VFN42_10925"
     CDS             2835..4004
                     /locus_tag="VFN42_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HET8997168.1"
                     /translation="MSNRLPSLDFDLGETADMLRAQVETFAADEIAPRAAAIDRDNDF
                     PADLWTKMGGLGLLGVTVEEEYGGAGMGYLEHVVAMEEISRASASVGLSYGAHSNLCV
                     NQIRRNGNEAQKRRYLPKLISGAYVGALAMSEPGAGSDVVGMRTRAEKRGDRYVLNGS
                     KMWITNGPNADVLVVYAKTDPDAGPRGITAFLIEKGFPGFSTAQKLDKLGMRGSNTCE
                     LVFQDCEVPEENVLGSVGRGVNVLMSGLDYERAVLAAGPLGIMQACLDVVLPYVHERR
                     QFGRPIGEFQLMQGKLADMYTTLSAARAYVHAVARACERGRTTRKDAAGAILYAAERA
                     TWMALEAIQCLGGNGYTSDYPTGRLLRDAKLYEIGAGTSEIRRMLIGRELFGETG"
BASE COUNT          684 a         1375 c         1401 g          635 t
ORIGIN      
        1 ccacgctgat cgtcaccgcg aattgccgga acagttcgcc ggagacgccg ggaatgaagg
       61 cgaccggcac aaacaccgac agcaacacca gcgagatagc gatcaccggg ccggtgatct
      121 gccgcatcgc cttcttggtt gcctccttgg ggccgagccc gggttcctcc gccatcaccc
      181 gttcggcgtt ctccaccacc acgatggcgt cgtcgaccac cacgccgatg cccagcacca
      241 tcgccagcag cgagatggta ttggcggaat agccgatcgc cagcagcacg gcgaaggtgc
      301 cgatcaggct taccggcacg gcgacgatgg ggatgatggt ggcgcgcacg ttgccgagga
      361 acaggaatac caccagcacg accaggccga aggcgatcgc cagggttttc accacctcat
      421 cgatggtcgc ggcgacgaag ccgctgctgt cgtagaacac gcgcgaccgc agtcccggcg
      481 ggtagcgctt gctcagctcc cgtagcaccc tggacacgcg ggcggaggtg gccaccgcgt
      541 tggcaccggg ggccaggtac agcccgatcg acacggccgg attgccgttc agccggcttt
      601 ccgtgtccat gctggcggcg cccaactcca cctgcgccac atcgtgcagc cgcagcacgg
      661 aaccgtcggg attggcgcga atgacgatgt tgccgaactg tttgggcgtg gtcagcctgc
      721 cctgggtctg cacattgagc tggaactgcg tgctgtccgg cgtcggacgg gcgccgacgc
      781 ggccaaccga ggcctgcacg ttctgcgcct ggatcgcctt gatgatatcg gacggcgcca
      841 gatgcagtgc caccagccgg ttcatgtcga accacacgcg catcgcgtag tccatcttgc
      901 cgaacaggaa cgcgtcgccg acgccgggcg tgcgcgccag ccggtccagc acgttgatgg
      961 tgacgtagtt gctgatgaac agcggatcct gcttgccgcc ttcgctgtag aattgcagga
     1021 attccaggat cgccgcggag cgtttgcgca gggtgacgcc ggcgcgctgc acctccggcg
     1081 gcagcttgct caaggcctgc tgcacccggt tgttgacgtt caccaggtcg atgtcgggat
     1141 tgctgcccag cgcaaagaac acgctcaggc tgtaactgcc gtcgttgccg ctgttcgact
     1201 tcatgtagat catcttgtcc acgccgacga ccgccgattc cagcggctgc gccacggtgg
     1261 attccaccac ggcggcagag gcgccgggga agctgccgga gacgatgatc tgcggcggca
     1321 caatgtcggg gaactgcgcc accggaatac ggaacagcga aagcacgccg gccatggtga
     1381 tcaggatggc cagcacgatc gccagccgcg gacgatcgac gaagacggcg gagatcatgc
     1441 ggtcagtgcc ccgcggccgg cggaccgccg ggcggcgtac tggctggacc gggggtcacc
     1501 gtgatgcccg gccgcacccg ctggatgccg ttcaccacga ccatctcgcc ctgcttcaag
     1561 ccggacacca ccaccgcggt cgtgggcgtg gactggccaa gctggatacg gcgttgctcg
     1621 atctggttgg acgggctgac cacgaacaca tagtcgcctt gctggtccga cagcaccgcg
     1681 gcgcggggta tgcccagagc ggtgaccggc tggacgcctt ccaccaggac cgtgacgaac
     1741 tcgccgtcga tcagcggccg gtccaccggc tggccgggct gcgccgggcg tcgcggcgga
     1801 ttggcaatgc tggcccgcag cacgatcgtg tcggtgttct gctgcacgct gggctcgaca
     1861 tagtcgatct tgccttccgg cttgtacagc gtgccgtccg gcaggcgcag cttcaccacc
     1921 acggcgttca ggccgccctt gccggcatag cgcttttcca ggtccacctc atcgcgcacc
     1981 gcgatgggga acagcacgta gatcggatcc tggctgacga tggtggccag ggcgccggaa
     2041 ccgggggaca cgacattgcc ggtgctgacc gtgctgcggc tgatctttcc ggcgatcggg
     2101 gcgtggatct cggtgtagcc caggttgatc cgtgccactt gcaactgcgc ctgggccgcc
     2161 agcacctgcg ccgccgccgc acgctgattg gcgaccgcat catccacgct ggaccgctgc
     2221 ccggccggcg tgtggagcaa ttgctgcgcg cgggcgagct ggatgttggc gttgaccagc
     2281 cgggcgctgg cctcggccac cgccgcctcc tgctgctgga cggcggcctc gaaggggccg
     2341 cgttccagcc ggaacagcag ttgaccggcg gcgacttcgg tgccctcggt gaacaggcgc
     2401 tggtccagga aggcggtgac acggggaacc agtgcgacgc ggtcgattgc ctgaacgcgg
     2461 ccgacatatt ccgaggtctc ggtgatcgcg gtctgcgtgg cgcgggccac gccgaccgcg
     2521 ggcgggccgc tcggcatctg cgcctgggca gcgacacagg acagcagcat cccggcgagc
     2581 aggccgaacg gcaggcgcaa cgacatcccg gctcttcccc atccgcacct gttgcggcac
     2641 atcaattacg cctttgctgg atggggagaa aaccgtcaat cgaattgcgg cgaggtcacc
     2701 gggccccggc ctggagcagc acggcgcgga gttcggcgac acggcacgcg cgccccggcc
     2761 cgcatctgcg gctgacttgc tttccgcgcc tcgcgtccta aaccctgctc tcccgcccgg
     2821 caggagaggc cgccatgagc aaccggttgc cgtcgctcga cttcgacctc ggcgagaccg
     2881 ccgacatgct gcgcgcgcag gtggaaacct tcgccgccga cgaaatcgcc ccgcgcgccg
     2941 ccgccatcga ccgcgacaac gattttcccg ccgacctgtg gaccaaaatg ggcgggctcg
     3001 gcctgctcgg ggtcaccgtg gaggaggaat acggcggtgc cggcatgggc tatctggaac
     3061 atgtcgtggc gatggaggag atcagccgag cctccgcgtc ggtggggctg tcgtacggcg
     3121 cgcattccaa cctgtgcgtc aaccagatcc gccgcaacgg caacgaggcg cagaagcggc
     3181 gctacctgcc gaagctgatt tccggcgcgt atgtcggcgc gctggcgatg agcgagccgg
     3241 gcgccgggtc cgacgtggtg ggcatgcgca cccgcgcgga aaagcgcggc gaccgctacg
     3301 tgctgaacgg atccaagatg tggatcacca acggcccgaa cgccgacgtg ctggtggttt
     3361 acgcgaagac cgacccggat gccggcccgc gcggcatcac cgccttcctg atcgagaagg
     3421 gtttccccgg cttctccacc gcgcaaaagc tggacaagct gggcatgcgt ggatcgaaca
     3481 cctgcgagct ggtgttccag gattgcgagg tgccggaaga gaacgtgctg ggcagcgtcg
     3541 gccgcggcgt gaacgtgctg atgagcgggc tcgactacga acgcgccgtg ctcgccgccg
     3601 ggccgctcgg catcatgcag gcctgcctcg atgtcgtact gccctatgtg cacgaacgcc
     3661 ggcagttcgg ccggccgatc ggcgaattcc agctcatgca gggcaagctc gccgacatgt
     3721 acaccacgct gagcgcggcg cgcgcctatg tccatgcggt cgcgcgcgcc tgcgagcggg
     3781 gacggaccac gcgcaaggat gccgccggcg ccatcctgta tgccgccgaa cgggcgacct
     3841 ggatggcgct ggaggcgatc cagtgcctcg gcggcaacgg ctataccagc gactatccca
     3901 ccggccgcct gctgcgcgac gccaagctgt acgagatcgg ggccggcacc agcgaaatcc
     3961 gccgcatgct gatcgggcgg gaattgttcg gcgaaaccgg ctgatttttg ctgcgccgca
     4021 acatcgcgca agaaaattgc gcgggccggg cggcgtcgcc ggggcggccg gggcggcggc
     4081 gtgcggcgcg cgacg
//
LOCUS       DASSZH010000500         4380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_983583, whole genome shotgun sequence.
ACCESSION   DASSZH010000500 DASSZH010000000
VERSION     DASSZH010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983583"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VFN42_10930"
     CDS             <1..573
                     /locus_tag="VFN42_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HET8997169.1"
                     /translation="LPHTKYGLPTYYIVAPAEASSNLARYDGVRFGLRADGADLRDLY
                     ERTRATGFGAEVRRRVLIGTYVLSAGYYDAYYLRAQKVRALILRDFTEAFAQVDALLT
                     PTAPSAAFAQGEKSDDPVQMYLNDVFTVPADLAGVPAMSVPGGLDANGLPLGLQVIAK
                     PFDEETMFAVGAALEQAAGFDGLPALRASG"
     gene            679..1212
                     /locus_tag="VFN42_10935"
     CDS             679..1212
                     /locus_tag="VFN42_10935"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET8997170.1"
                     /translation="MTDQFEFGPEWRYELVDGVIVGHAAPSADHAAILAGLTGALTRR
                     MEDRTDGCRPEVGSGAAPKNQQRATARIPDAMIRCREHPRVTFDIVSPSELRAWRARD
                     RHRQHLQDVEGVQEIVEVYQDEAAIHVYRKGPADAWVFEAINGLDATLVLGSVALEIP
                     LVEIYRFVVLEERETPL"
     gene            1209..2660
                     /gene="gatB"
                     /locus_tag="VFN42_10940"
     CDS             1209..2660
                     /gene="gatB"
                     /locus_tag="VFN42_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET8997171.1"
                     /translation="MSYVIEGSTGSWEVVVGLEVHAQVISQAKLFSGAATAFGAAPNT
                     QVSFVDAAFPGMLPVINRECVTQAVRTGLGLNAHIHLVSRFDRKNYFYADLPAGYQIS
                     QYEHPIVGEGAVEIELADGSTRQIGITRLHLEQDAGKSLHDQHPTKSYIDLNRAGVAL
                     MEIVSEPDIRSPEEAGAYLRKLRSILRYLGTCDGNMEEGSMRADVNVSVRKAGEPYRT
                     RCEVKNVNSVRFVMQAIEAEAARQVEVWESGGTVQQETRLFDSARGTTRSMRSKEDAH
                     DYRYFPDPDLLPLVLEQDWVTALKQSLPELPDAKKARFMREYELSTYDAGVLVAERAT
                     AEYFEAVAKGHNGKQAANWVMGDLFGALNRLGKTIEDSPVSAAALGRLLDLMADNTIN
                     GRIAKDVFDAMVETGKDPATIVDERGLRQVTDTSAIDEAVDAILAANADKVAEYRSGK
                     DKLFGFFVGQVMRTMKGKGNPALINETLKRKLS"
     gene            complement(2691..>4380)
                     /locus_tag="VFN42_10945"
     CDS             complement(2691..>4380)
                     /locus_tag="VFN42_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997172.1"
                     /translation="APAAITGFVPGDTLLVADSSSNIVTQLSMLEGLAQSGRLTAIHL
                     TDAGTPTLSLSAAQRSAGADVLARIATPYQLASGVTWIAEQSGSFDDAAQWSGGSVPD
                     ADADAVIDSAGQPTVTISGTHALHTLANLAGHFILTGGILQVGQLDNESSLAWNGGSI
                     ALDGSDADSSGLVNGAGATLSILANGQQIGGTGDFANAGAMLISGTGQVALDTPLVNT
                     GNLVVTQGTLELNAGGRSTGSIVAQPGGNIVFGGGSFAVTGGYAGNSTTISGGTLDVS
                     AVAGNFRTLLLSAGALALSTAAADSNGPLLQAGGTLDGSGSFVAFAGAQLSGGVQTGP
                     GSTRLLGQSTISGTALDGGRTLRNDGALTWSSGDILAGGGDTAAVIHGATLSNTGVLR
                     ITAQATIGPANDGSGMVTNAGLISVQAGAGSATMKASLLNAGIVVVESGTLSLAGPVS
                     GGGLFEIAGGAALAFANLVGAGETLRFLGGGGTLAIDQTGPFAMPVAGFVAGDVLDLT
                     ALDFAAGPSARLSGGTLTVSDGTHSAMLSLIGSYAASGFQLTGDAHGGVLAQYS"
BASE COUNT          748 a         1448 c         1465 g          719 t
ORIGIN      
        1 ctgccgcaca cgaaatacgg cctgcctacc tactacatcg tcgccccggc cgaggcctcg
       61 tccaacctgg cgcgttacga cggcgtgcgc ttcggtctac gtgcggacgg tgcggacctt
      121 cgcgatctct atgagcgcac ccgtgccacg ggcttcggcg cggaggtgcg gcggcgcgtg
      181 ctgatcggta cctatgtgct gtcggccggt tactacgacg cctactatct tcgcgcgcag
      241 aaagtgcggg cattgatctt gcgtgacttc accgaggcat tcgcccaggt ggacgcgctg
      301 ctgaccccca ccgcgccgtc cgccgccttc gcccaaggcg agaagtcgga cgatccggtg
      361 cagatgtatc tgaacgatgt tttcaccgtt ccggcggacc tggccggcgt gccggcgatg
      421 tcggtaccgg gcgggctgga tgccaacgga ctccccctgg gcctgcaggt gattgccaag
      481 ccgttcgacg aggagacgat gttcgcggtc ggcgccgcgc tggagcaggc ggcggggttc
      541 gatgggttgc cggcgctacg ggcgagtgga taggccgcgc ttgccgcccg gctttcgtgg
      601 tatggtggtg tcggctcgga ggttcggatg agcacgacag caagaattga aagaacttgg
      661 acggcggagg aattcctcgt gaccgaccag ttcgaattcg gtcctgagtg gcgctacgaa
      721 ctcgttgacg gcgtgatcgt tggtcatgcc gcgccgagcg ccgatcacgc cgcgatcctg
      781 gcagggctga caggggcgct gacgcggcgt atggaagaca ggacggacgg ctgccgtccg
      841 gaggtcggca gcggcgccgc gccaaaaaat cagcaacgcg ccaccgctcg cattccggac
      901 gccatgatcc gctgccggga gcacccgagg gtgacgttcg atatcgtctc cccctcggag
      961 ctgcgcgcat ggcgagcccg cgaccgccac aggcagcatc tgcaggacgt cgagggcgtg
     1021 caggagatcg tcgaggttta ccaggacgag gcggcgatcc acgtttaccg gaaaggcccg
     1081 gctgacgcgt gggtcttcga agcgatcaac ggactcgatg cgacccttgt tctggggagt
     1141 gtcgcactgg agattccgct ggtcgagatt tatcgctttg tggtgcttga ggagcgcgaa
     1201 acaccattat gagctacgtc atcgaaggca gcaccggatc ctgggaggtt gttgtcggcc
     1261 tggaagtaca cgcccaggtg atcagccagg ccaagctgtt ctccggcgcc gccacggcgt
     1321 tcggcgcggc acccaacacc caggtcagct tcgtcgatgc cgccttcccg ggcatgctgc
     1381 cggtgatcaa tcgcgaatgt gtcacccagg cggtgcgcac cggcctgggg ctgaacgcgc
     1441 atatccacct ggtcagccgc ttcgaccgca agaactactt ctatgccgat ctgcccgccg
     1501 gctatcagat cagccagtac gaacacccga tcgtcggcga aggcgcggtg gagatcgaac
     1561 tcgcggacgg cagcacccgc cagatcggca tcacccggct gcatctggag caggacgccg
     1621 gcaagtcgct gcacgaccag cacccgacga aatcctacat cgacctcaac cgcgccgggg
     1681 tggcgctgat ggagatcgtg tcggagcctg acatccgctc cccggaggaa gccggcgcct
     1741 atctgcgcaa gctgcgttct atcctgcgct acctgggcac ctgcgacggc aacatggagg
     1801 aaggctccat gcgcgccgac gtcaacgtgt cggtacgcaa agccggcgaa ccgtaccgca
     1861 cccgctgcga ggtgaagaac gtcaactcgg tccgcttcgt catgcaggcg atcgaggcgg
     1921 aagccgcgcg ccaggtggag gtttgggaat ccggcggcac ggtgcagcag gaaacccggc
     1981 tgttcgacag tgcccgcggc accacccgca gcatgcggtc gaaggaggac gcccacgact
     2041 accgctactt ccccgatccc gacctgctgc cgctggtgct ggaacaggac tgggtcaccg
     2101 cgctgaagca gtcgctgccg gaactgccgg acgccaagaa ggcgcgcttc atgcgcgagt
     2161 acgagttgtc cacctacgac gccggggtgc tggtcgccga acgcgccacc gccgagtatt
     2221 tcgaggcggt ggcgaaaggc cacaacggca agcaggcagc caactgggtg atgggcgacc
     2281 tgttcggcgc gctgaaccgc ctcggcaaga ccatcgagga ctcccccgtt tccgccgcgg
     2341 cgctcggccg cctgctcgac ctgatggcgg acaacacgat caacggtcgc atcgccaagg
     2401 acgtgttcga cgcgatggtg gagaccggca aggaccccgc caccattgtc gatgagcgtg
     2461 gcttgcgcca ggtcaccgat accagcgcga tcgacgaggc ggttgacgcc atcctggcgg
     2521 ccaatgccga caaggtggcg gagtaccgca gcggcaagga caagctgttc ggcttcttcg
     2581 tcggccaggt gatgcggacg atgaagggca agggcaatcc agcgctgatc aacgagacgc
     2641 tgaagcgaaa gctgtcctga tcggccgacc ggggaccgcc aggcgcgggg tcacgagtac
     2701 tgcgccagca cgccgccatg tgcatcaccg gttagctgga accccgatgc ggcatagctg
     2761 ccgatcagcg acagcattgc gctgtgcgtc ccgtcgctca cagtcagtgt gccgccgctg
     2821 agccgggcac tggggccggc ggcaaagtcc agtgctgtca gatccagcac gtccccggcg
     2881 acaaaaccgg cgaccggcat tgcgaaaggg ccggtctgat cgatcgccag tgttccgccg
     2941 ccgccgagga agcgcagcgt ttcgcccgca cccaccaggt tggcgaacgc cagtgctgca
     3001 ccgccggcga tctcgaacag cccgccacca ctgaccgggc ctgcaaggct cagggtgccg
     3061 ctctcgacca cgacgattcc ggcgttcagc agggaggcct tcatggtggc ggagccggcg
     3121 ccggcctgca ccgagatcag gcccgcattc gtcaccatgc cgctgccatc attggccggg
     3181 ccgatcgtcg cttgcgcggt gatgcgcagc acgccggtat tgctcagggt cgcgccatgg
     3241 atcacggccg cagtgtcgcc gccgcccgcc aggatgtcgc cggatgacca ggtcagcgcg
     3301 ccgtcattcc gcagcgtgcg tccgccatcg agcgccgtgc cgctgatcgt ggactggccg
     3361 agcagtcgcg tgctgccggg gccggtttgt actccgccgg aaagctgggc accggcgaag
     3421 gcaacgaaac tcccgcttcc gtccaatgtt ccaccggcct gcagcagcgg gccgttgctg
     3481 tctgccgccg cggtgctcag ggcaagggcg ccggctgata gcagcagcgt tcggaaattc
     3541 ccggccactg cggagacatc cagcgtaccg ccgctgatcg tggtgctgtt tccggcatag
     3601 ccgccggtta ccgcgaagct gccgcccccg aacacgatat tgccgcctgg ctgcgccacg
     3661 atgctgccgg tcgatcggcc gccggcgttc agttccagcg tgccttgtgt caccaccagg
     3721 ttgccggtgt tcaccagcgg cgtatccaac gcgacctgcc cggtgccgct gatcagcatg
     3781 gctccggcat tggcaaaatc gccagtgccg ccgatctgct gtccgttggc caggatgctc
     3841 agcgtcgcgc cggcaccatt caccaggccc gagctgtcgg cgtcgctgcc gtccagcgcg
     3901 atgctgccgc cgttccaggc caggctggac tcgttgtcga gttgcccaac ctgcagaatg
     3961 ccgccggtca ggatgaaatg tccggcaaga ttggccagcg tatgcagcgc gtgggtgccg
     4021 ctgatcgtca ccgtgggttg gccggcggaa tcgatcaccg cgtccgcatc cgcatcgggc
     4081 acgctgccgc cgctccattg cgccgcgtcg tcgaagctgc cggactgctc ggcgatccag
     4141 gtgacgcccg atgccagttg gtagggcgtg gcgatcctcg ccagaacatc ggcacccgcg
     4201 ctgcgttgcg cggcactcag tgacagcgtc ggcgtgcctg catcggtcag gtggatcgcg
     4261 gtgagacgac ccgactgggc cagtccttcc agcatggata gctgggtgac gatgttgctg
     4321 gaactgtccg ccaccagcag cgtatcgccg ggcacgaagc cggtgatcgc cgccggagcg
//
LOCUS       DASSZH010000501         2805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_96979, whole genome shotgun sequence.
ACCESSION   DASSZH010000501 DASSZH010000000
VERSION     DASSZH010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96979"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="VFN42_10950"
     CDS             <1..986
                     /locus_tag="VFN42_10950"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003188.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HET8997173.1"
                     /translation="KVTQHADRLAALMVTYPSTHGVFEVAIRDICAVVHAVGGQVYMD
                     GANLNAQVGLTSPGAIGADVCHLNLHKTFCIPHGGGGPGVGPLGVAAHLVPHLPNHPL
                     RADAGPASGFGPVSAAPFGSAMILPISYAYIRMMGAAGLKRATEVAILNANYIAHRLR
                     PHYPILYTGPGGMVAHECIVDCRGFQAEAGVLVEDVAKRLMDFGFHAPTMSWPVAGTL
                     MIEPTESESKAELDRFCDAMIAIRAEIAAIGAGKLDRKDNPLKNAPHSARELTGDTWT
                     HGYSREQAAFPLPWVAAAKYWPPVKRVDNVYGDRNLVCSCAPLEAYAQAAE"
     gene            complement(1257..1463)
                     /locus_tag="VFN42_10955"
     CDS             complement(1257..1463)
                     /locus_tag="VFN42_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997174.1"
                     /translation="MLSIGAISLQGCVYDPYTGYYEPYAYPAYGYYPYSYGYYPPVSG
                     SVVIGGGWNWDDHRGWDGRRGWRR"
     gene            complement(1946..2449)
                     /gene="nuoB"
                     /locus_tag="VFN42_10960"
     CDS             complement(1946..2449)
                     /gene="nuoB"
                     /locus_tag="VFN42_10960"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HET8997175.1"
                     /translation="MLKTLLQGLRTPLTEPRPDPDPELTALAATLDATARRKLGRGLA
                     IRAVDAGSCNGCELEIHALNNAFYDLERFGLRFVASPRHADVLLVTGPVTKNMQEALL
                     RTWRATPDPKWVVAAGDCAANGHVFAGSYAVAGGVSSTLPVDLVIRGCPPTPMQLLSG
                     LLTLMQR"
     gene            complement(2463..>2805)
                     /locus_tag="VFN42_10965"
     CDS             complement(2463..>2805)
                     /locus_tag="VFN42_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase expression protein HypE"
                     /protein_id="HET8997176.1"
                     /translation="GDVNARAWIRAREIEQSLSMIEQLLEGLPGGEIRAFLEDAPLDP
                     GEGMALVEAFRGDVLVWLRVAGGCVERCHLRDASWFQWPLLEVVIEGNIVADFPLCNK
                     SFNCSYSGHDL"
BASE COUNT          468 a          943 c          941 g          453 t
ORIGIN      
        1 ccaaggtgac gcagcacgcc gaccggctgg ccgcgctgat ggtcacctat cccagcacgc
       61 acggcgtgtt cgaggtggcg atccgtgaca tctgcgcggt ggtgcacgcc gtcggcgggc
      121 aggtgtacat ggatggcgcc aatctgaacg cacaggtcgg actgacctcg ccgggcgcga
      181 tcggcgcgga tgtctgccat ctgaacctgc acaagacctt ctgcatcccg catggcggtg
      241 gcggtcccgg cgtggggccg ctcggcgtgg cagcgcatct ggtgccgcat ctgccgaacc
      301 atccgctgcg cgccgatgcc ggacccgcca gcgggttcgg cccggtttcc gcggcaccgt
      361 tcgggtcggc gatgatcctg ccgatctcct atgcctatat ccggatgatg ggcgccgcgg
      421 gattgaaacg cgcgaccgag gtggcgatcc tcaacgccaa ctacatcgcg catcggctgc
      481 ggccgcatta cccgatcctg tacaccggcc ccggcggcat ggtggcacac gaatgcatcg
      541 tggattgccg cggcttccag gcggaagccg gcgtgctggt ggaggacgtc gccaagcggc
      601 tgatggattt cggcttccat gcgccgacca tgtcctggcc ggtggccggc acgctgatga
      661 tcgagccgac ggaaagcgag agcaaggctg aactcgaccg gttctgcgat gcgatgatcg
      721 cgattcgggc agagatcgcg gcgattggtg ccggcaagct ggaccgcaag gacaacccgc
      781 tgaagaatgc gccgcattcc gcgcgcgaac tcaccggcga tacctggacc cacggctatt
      841 cgcgtgagca ggcggcgttc ccgctgccct gggtggcggc ggcaaagtac tggccgccgg
      901 tcaagcgggt ggacaacgtg tatggcgacc gcaacctggt gtgcagttgc gcccccttgg
      961 aggcctacgc ccaggccgcg gagtaatagc agcccgcggg gcattcggct cccgggcgtg
     1021 ccgcacgccg tcgtgccggc gcatgccggc acccacggct tgccgtggag ggatgcggaa
     1081 aactcgtgga tggcgtgcca cggactgtct tcgggcgcgc ctgcggcgcg atcggtggga
     1141 ccgccacaac ccttcacggc tgacgctgcg tcatcagcaa cgcgggtgcg gtatcaggct
     1201 atggtcccgc tccgctggcc acgagcggag agggacctat tcgccacgca cgcggatcag
     1261 cggcgccagc cgcgacgccc gtcccagcca cggtggtcgt cccagttcca cccgccgccg
     1321 atcaccacgg aaccactgac gggcgggtag tagccataac tatagggata gtagccgtag
     1381 gcgggataag cgtagggctc ataatagccc gtatacggat cgtagacgca gccctgcagc
     1441 gaaatcgctc cgatggatag caatgccgtg agaagaattg tccgaacaga tgccttgaac
     1501 atatcagcct ccggccaacg cactccaact tgccggcagc agggcacagc gtcgtccggg
     1561 agcgcgttgg caacggaccg gcgacctgcg ccttgggaag cgctcggctc gcagatgctt
     1621 ctatgccagc aacgccacgc atggacattg atctgcatca aatatcaagc cattcccggc
     1681 cacctgggat cgcccgggta accagtctgc tagtgcatgc attagccgac tcgctccgga
     1741 tctgcatgac cgcgccggtg cccgcgcttc cccctgccct ggacgccatc gtggaacggt
     1801 tgtggcaaga tgcctgcgcg cgaatggccc gcggcagcgc gggtcgcttg ttcaacggct
     1861 gggagttcag cgccggcgtt cctgctgcgg gcggggttgc tggccggcgc cccgtgacgc
     1921 cccgtgacca tgggcgtccc gcctattatc gctgcatcag cgtcagcagg ccgctcagca
     1981 actgcatcgg cgttggcgga cagccgcgga tcaccaggtc caccggcagg gtgctgctga
     2041 cgccaccggc cacggcataa ctgcccgcga acacgtgccc gttcgctgca cagtcccctg
     2101 ctgccaccac ccatttcgga tcgggtgtcg cgcgccacgt acgcagcagc gcctcctgca
     2161 tgttcttcgt caccggcccg gtgacgagca gcacgtcggc atggcgcggc gaggcgacga
     2221 agcgcaggcc gaagcgctcc aggtcgtaga acgcgttgtt cagcgcgtgg atttccagtt
     2281 cacagccgtt gcaggagccg gcatcgaccg cgcggatcgc caggccgcgg ccaagcttgc
     2341 ggcgggcggt ggcgtcgagt gtcgcggcaa gcgccgtgag ttccgggtcc ggatcggggc
     2401 gcggttcggt cagcggcgtg cgcaggccct gcagcagggt tttcagcatg gcgggtacgc
     2461 tgtcacaggt cgtgccccga atacgagcag ttgaagctct tgttgcaaag cgggaagtcg
     2521 gcgacgatgt tgccctcgat caccacctcc agcagcggcc actggaacca ggacgcgtcg
     2581 cgcaggtggc agcgctcgac gcagccgccg gcgacgcgca gccataccag cacgtcgccg
     2641 cggaacgctt ccaccagcgc catgccttcg cccggatcca gcggcgcgtc ttccaggaag
     2701 gcgcggatct cgccgccggg cagaccctcc agcagctgtt cgatcatgga caggctttgc
     2761 tcgatttccc gcgcccggat ccaggcacgc gcgttaacgt cgccc
//
LOCUS       DASSZH010000502         2574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_165477, whole genome shotgun sequence.
ACCESSION   DASSZH010000502 DASSZH010000000
VERSION     DASSZH010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165477"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="VFN42_10970"
     CDS             complement(<1..1221)
                     /locus_tag="VFN42_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997177.1"
                     /translation="MDVIAEAASAGGVADGQAFAGRARVLAVWAALSGLVLLPVLLVP
                     VPALVDYANHLARNWILARGSHIPLLLTNYVIHWRVIADLAMDVMVRSLSTVMPVEAA
                     GKLFVILTVLGLIAGTAALHKALWGQVGLWPAVCVFFVYDSVLYWGIVDCLFGMGVFL
                     LAFSLWIVSAPWRPVARLLVFSLIACGLLLLHLFAYGVYGLAIGSYEFGLFWRRRSLR
                     RFGMCCLWGTQFLPSVVLWWIAFRNGEGTFTEYGNFAAKLYALMSAVNFGQQAVLLDW
                     ACVLLVVVFLIFALALKWLHIHPAMRLPLVVMLIAAALMPSWLTGSWGADFRLPTALA
                     FLTIAGSELRLPRRTAFVFAGTALLLLGLRVWGTSAGWLDYNANLREFRRAAQVITPG
                     SRMLVVEVPPTGAEK"
     gene            complement(1299..1970)
                     /locus_tag="VFN42_10975"
     CDS             complement(1299..1970)
                     /locus_tag="VFN42_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010515091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET8997178.1"
                     /translation="MSTSVSDIAANLQRVRERIAEAAIRAGRKPEAVTLVAVSKTKPV
                     EAVRAALQAGQTVFGENRVQEAAAKFPALRKEHAFNLHLIGGLQTNKAREAVALAEVI
                     ETLDRPRLADAIAAAIAKEGRTPTLLVEVNVGNEPQKSGVPRDDADGFIADCKGRFGG
                     ALAGLMCVPPHGEDPKPHFEWLADRAARHGLAVLSMGMSADFETAIACGATWVRVGSA
                     IFGAR"
     gene            2048..>2574
                     /locus_tag="VFN42_10980"
     CDS             2048..>2574
                     /locus_tag="VFN42_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET8997179.1"
                     /translation="MIDPRLLAWARHTARRKAGARPLPPLWLFTDFHRMPDPRPAVAR
                     LPRGLAGVVFRHDEDPRRALLGRDLARLCRTRRLMLVVAGDVRLAAALGAGVHLRGGH
                     WPGPTRRRGMVTSSAHRLPDLRRAAAAGARLAFLSPVFPTASHPGAPALGPLRWMRMA
                     RAAPLPVAALGGVQPA"
BASE COUNT          437 a          955 c          832 g          350 t
ORIGIN      
        1 cttttcggcg ccggtcggag gcacctccac caccagcatc cgcgacccgg gcgttatgac
       61 ctgcgcggcg cggcgaaact cccggagatt ggcgttgtaa tccagccagc ctgcgctcgt
      121 gccccagacg cgcagtccca gcagcagcaa agccgtcccg gcaaagacaa acgcggtgcg
      181 gcgcggcagg cgcagctctg aaccggcaat ggtcaggaat gccagggcag tgggcaggcg
      241 gaaatccgcg ccccaactgc cggtaagcca gctcggcatg agcgcggccg cgatcagcat
      301 aaccaccagc ggcagacgca tcgccggatg gatatgcagc catttgaggg ccagcgcgaa
      361 aatcaggaac accaccacga gcagcacgca cgcccagtcg agcaggactg cctgctgtcc
      421 gaagttgact gctgacatca gcgcatacag cttcgcggcg aagttgccgt actcggtgaa
      481 cgtgccttcg ccgttccgga acgcgatcca ccacagcacc acgctgggca gaaactgcgt
      541 gccccacagg cagcacatgc cgaagcgccg cagcgaacgg cgccgccaga acaacccgaa
      601 ttcgtagctg ccgattgcca atccatacac gccgtacgca aacaggtgca gcagcagcaa
      661 tccacaggcg atcagggaaa acaccagcag gcgtgcaacg ggccgccaag gtgcgctgac
      721 gatccacaag ctgaacgcaa gcaggaacac gcccatcccg aacaggcaat cgactattcc
      781 ccagtacaac accgagtcat agacgaagaa gacgcaaacc gccggccaca gtcccacctg
      841 gccccacagc gccttgtgca gcgctgccgt acccgcgatc aggcccagca cggtcaggat
      901 gacgaacagc ttgcccgccg cctccaccgg catgaccgtg gacagcgacc gcaccatcac
      961 gtccatcgcc agatcggcta tcacccgcca atggatgacg tagttcgtca acagcagcgg
     1021 gatgtgcgat ccgcgcgcca ggatccagtt gcgcgcgagg tggttggcat aatccaccag
     1081 ggccggcacc ggcaccagca gaaccggcag caacaccagc cccgacagcg ccgcccacac
     1141 cgccagaacc cgcgcccggc cggcgaaggc ctgcccgtcg gcaacgcctc cggcagaagc
     1201 ggcttcagcg atcacatcca agacacgtcg gctcctctgg atcagggcta cactctgcat
     1261 gcagccgctt gcatcgcggt tgcagccctc caggctgcct accgcgcgcc gaaaatcgcg
     1321 ctgcccacgc gcacccaggt ggcgccgcaa gcgatcgcgg tctcgaagtc cgccgacatc
     1381 cccatcgaca gcaccgccag cccgtgccgg gcggcccggt cggccagcca ttcgaagtgc
     1441 ggcttcgggt cttcgccgtg cggcggcacg cacatcagcc cggccagagc gccgccgaac
     1501 cggcccttgc aatcggcgat gaacccgtcc gcgtcgtcgc gcggcacgcc ggatttctgc
     1561 ggctcattgc cgacgttcac ctccaccagc agggtcggcg tgcggccctc cttcgcgatg
     1621 gccgcggcga tcgcgtcggc caggcgcggg cggtccagcg tctcgatcac ctcggccagc
     1681 gccaccgcct cgcgcgcctt gttggtctgc aggccgccga tcaggtgcag gttgaaggcg
     1741 tgctccttgc gcagcgccgg gaacttcgcc gccgcctcct gaacgcggtt ctcgccgaac
     1801 accgtctggc cggcctgcaa ggctgcgcgc accgcctcca ccggcttggt cttcgatacc
     1861 gcgaccagcg tcactgcctc cggcttgcgg ccggcgcgga tcgcggcctc ggcgatccgc
     1921 tcgcgcacgc gctgaagatt ggcggcgata tcggaaacac tggtgctcat ggcgtcatct
     1981 cggtgcatgg gcgcttgcgc cggcgggatt ggtggggttc aggtatcagc aatcgagagg
     2041 caaggcgatg atcgatccgc ggctgctggc ctgggctcgc cacacggcgc ggcggaaggc
     2101 cggcgcgcgg cccctgccgc cgctctggct gttcaccgac ttccaccgca tgccggatcc
     2161 ccgcccggcg gtggcgcgcc tgccgcgtgg gctggcgggg gtggtgttcc gccacgacga
     2221 ggacccgcgg cgcgctctcc tcgggcgcga cctcgcccgg ctgtgccgaa cccggcgcct
     2281 gatgctggtg gtggcaggcg acgtacgcct ggccgcggcg ctcggcgccg gcgtgcatct
     2341 gcgcggcggg cactggccgg gtcccacccg ccgccgcggc atggtcacca gctcggcgca
     2401 ccggctgccg gacctgcgcc gggccgcggc agccggggcg cgtctcgcat tcctgtcgcc
     2461 ggtgttcccc accgccagcc atcccggtgc gccggcgctc ggtccgctgc gctggatgcg
     2521 catggcgcgt gcggcgccgt tgccggtggc ggcgcttggc ggcgtgcagc cggc
//
LOCUS       DASSZH010000503         3706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1563136, whole genome shotgun sequence.
ACCESSION   DASSZH010000503 DASSZH010000000
VERSION     DASSZH010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563136"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..745
                     /locus_tag="VFN42_10985"
     CDS             143..745
                     /locus_tag="VFN42_10985"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HET8997180.1"
                     /translation="MTRRLPFILGGCLLALVLAWGAGFVWFVHLATRPVPPPPHADAI
                     VAFTGGADRVATALHLLVEGKAAALLLSGIGGGAELRDLARRADLNPRPIAARVTIGR
                     EALTTHGNARETAAWARNRSIHSLIVVTAFYHMPRALAELRRALPDVRLYPDPVLMPR
                     HGGIGHLVTLRLMAEEYTKYLAVVSGLSSLVPLRLHGASL"
     gene            742..1470
                     /locus_tag="VFN42_10990"
     CDS             742..1470
                     /locus_tag="VFN42_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET8997181.1"
                     /translation="MTVLRSALYNLFFFGVSSVLAVYGMALRLLAPHRIIDLARLWAR
                     LMLGGARVICGIRVQVTGLDRLPDGAALLASRHQSAFDTLVWLLLAPRCCYVLKQELL
                     RIPVFGWLVPPTGMIAVDRSGGSAALRGLMRDGAQAARDGRQIVIFPEGTRAAPGAVL
                     PLQPGIAALAASTGLPVIPVVTDSGLYWGRRAFRKRPGTIHIVLLPALPAGLPRPELM
                     RRLTEALRSEIRPARASDYPSSSG"
     gene            complement(1449..2177)
                     /locus_tag="VFN42_10995"
     CDS             complement(1449..2177)
                     /locus_tag="VFN42_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET8997182.1"
                     /translation="MAYEFFYWPEIQGRGEFVRLALEDAGADYIDVARGSERDGLGVP
                     AMLALMRGEGTSHIPFAPPFLRDGSVIVSHTAAILLYLGGRIGLAPSDEDGRMWAHQL
                     QLTITDIVKEVYDAHHPIDEDKWFHEQKDEAIARAAVFRKDRMPKFLGWFERVLTANP
                     AGSHHLVGDAVTYADLSLFQLAEGLRYAFPRASKRTLAKLPRVSALHQAVTERPRLKA
                     YLASERRVAFNETGIFRHYPELDG"
     gene            complement(2575..2739)
                     /locus_tag="VFN42_11000"
     CDS             complement(2575..2739)
                     /locus_tag="VFN42_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997183.1"
                     /translation="MNDNFIKPDGTAWEYEASRFESARHLQLLRQAGFAAPDSLQLFE
                     HELDTPTAAK"
     gene            3012..>3706
                     /locus_tag="VFN42_11005"
     CDS             3012..>3706
                     /locus_tag="VFN42_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET8997184.1"
                     /translation="MDLAFTEEELAFREEARRFFRSEIPQSIRDKVAEGEGLTRDDMI
                     TSQRILNAHGWATPNWPVQYGGKDWSSVQVYLYQDEMQQANVPPPIAFNVSMVGPVIA
                     QFGSEEQKQRFLPKTANADIFWCQGFSEPGAGSDLAGLRTRAERKGDKYVINGQKIWT
                     TLAQYADWIFCLCRTDPAAKKQEGISFILVDMKTPGITVRPIITIDGGREVNEVFFDN
                     VEVPAENLVGQEN"
BASE COUNT          565 a         1249 c         1264 g          628 t
ORIGIN      
        1 accacctcgc cgctctgggt gttcaccgca ctgccgccga agctctggct gatcctgcct
       61 gggctgcccc tggcggccgc ggcgatcggc tacgcgaccg cgcaaggcac ggtgcgacgg
      121 tggctgcaac ggctgccctg acgtgacccg ccgcctgccg ttcattctgg gaggatgcct
      181 gctggccctg gtgcttgcct ggggggccgg gttcgtctgg ttcgtgcacc tggccacccg
      241 cccggtgccg ccgccgccac acgccgacgc gatcgtcgcc ttcaccggcg gcgccgaccg
      301 ggtggccacg gcgctgcacc tgctggtcga aggcaaggcc gcggcgctgc tgctatccgg
      361 tatcggcggc ggcgcggagc tgcgcgacct ggcgcggcgt gccgacctca atccccggcc
      421 gatcgccgca cgggtgacga tcggccgcga agccctgacc acgcatggca atgcgcggga
      481 aactgcggcc tgggcgcgca accggtcgat ccactcgctg atcgtggtga ccgcgttcta
      541 tcatatgcca cgcgcgctgg cggaactgcg ccgggcgctg ccggatgtgc ggctgtaccc
      601 cgatcccgtg ctgatgccgc ggcatggcgg cattggccat ctggtcacgt tgcggctgat
      661 ggccgaggaa tacacgaaat acctcgccgt cgtatcgggc ctgtccagcc tggtaccgct
      721 gcggctgcat ggggcctcgc tatgacggtg ctgcgttcgg cgctgtacaa cctgttcttc
      781 ttcggggtca gcagcgtgct ggcggtgtac ggcatggcgc tgcgcctgct ggcgccgcac
      841 cggatcatcg atctggcgcg gctctgggct cggctgatgc tgggcggcgc ccgggtgatc
      901 tgcggcatcc gggtgcaggt gaccgggctg gaccggctgc cggatggcgc ggcgctgctc
      961 gcgtcccgcc accagtcggc cttcgacacg ctggtctggc tgctgctggc gccgcgctgc
     1021 tgctacgtgc tgaagcagga gctgctgcgc atcccggtgt tcggctggct ggtgccgccg
     1081 accggcatga tcgcggtgga ccggtccggc ggcagcgcgg cgctgcgcgg cctgatgcgc
     1141 gacggggcgc aggcggcacg ggacggccgg cagatcgtga tcttccccga gggcacccgc
     1201 gcggcgcccg gcgccgtcct gccgctgcag cccgggatcg ctgcgctggc cgccagcacg
     1261 gggctgccgg taatcccggt ggtgaccgat tccggcctgt actggggccg ccgcgccttc
     1321 cgcaagcggc ccggcaccat ccatatcgtg ctgctgcccg cgttgcccgc cggcctgccg
     1381 cggcccgaac tgatgcggcg gctgaccgag gcgctgcgct cggagatccg tcccgcccgt
     1441 gcgtcggact atccatccag ctccggatag tgccggaaga ttccggtttc gttgaaggca
     1501 acccgccgtt cgctcgccag gtacgccttc aggcgcggcc gctccgtcac cgcctggtgc
     1561 agggcgctca cccgcggcag ttttgccagt gtgcgtttcg acgcgcgtgg aaacgcgtag
     1621 cgcaggccct cggccagctg gaacagcgac aggtcggcat aggtgacggc atcgccgacc
     1681 agatgatgcg aacccgcggg gttcgccgtc agcacccgct cgaaccagcc gaggaacttc
     1741 ggcatccggt ccttgcggaa caccgcggcg cgcgcgatgg cctcatcttt ctgttcgtgg
     1801 aaccacttgt cctcgtcgat cgggtgatgc gcgtcgtaca cctccttcac gatgtcggtg
     1861 atcgtgagct ggagctgatg cgcccacatc ctgccgtctt catcgcttgg tgcgagtccg
     1921 atgcggccgc ccagatacag caggatcgcc gccgtatggc tgacgatcac gctgccgtcg
     1981 cgcaggaacg gcggggcgaa gggaatatgc gatgtgcctt cgccgcgcat cagcgccagc
     2041 atggcgggga caccgagtcc gtcgcgttcc gatccgcgcg ccacgtcgat gtagtcggct
     2101 ccggcatctt ccagtgccag gcggacgaat tcgccgcgtc cctggatttc cggccagtag
     2161 aagaactcgt aagccatggc gtgcctctcc tgcgaagagc ggtgagaccc acatcgatgc
     2221 cacgaacggt tcggcagcag caagcctcac ttctttgccg cagatgcgca gcgctgccag
     2281 gatttgtcgg cggcacgggc agtccggggg aacgccccaa gttggggcaa atcgcagcga
     2341 agcacagtat cttcaataca gaggaagcgg gcgagggcca ccgaggccac cgagtcggtg
     2401 cttgcgcggc ggatgtggaa caatcaccga ctcatgtgcc ctttgtggtc ctccctgctt
     2461 cctctgtgtt aaaaacactt gccctgcttg ctacaggccg caccggcgat gggctccgat
     2521 aaggcgcttg gcgcaggttt catctgcgtg ccttcgcgtc gatccgcgcc ggcgctattt
     2581 cgccgcggtc ggcgtatcga gctcatgctc gaaaagctgg agtgagtccg gtgccgcgaa
     2641 gccggcctgg cgcaacaact gcaaatgccg ggcgctctcg aaccggctgg cttcgtactc
     2701 ccacgcggtg ccatccggct tgatgaagtt gtcgttcacc aggatgccgc gttcatgaga
     2761 cgcggctcca tgcactgatg gcgtgcagca gtggaagtta tctgcgaacg cgcagcaacg
     2821 cacgccttct cccatccgcg ccaccggtcg ggctggtgtt cgctggcgcc gagacggatg
     2881 cggtgcaggc ggctggtccg ccgcccgcca gccccaagga tgcgctcgcg gcggctgagg
     2941 ggcgggcgtt gacgcgctgc cggcctgccg gtactcacca gtagctgcac cacggcacgg
     3001 agggaaccgc catggacctg gccttcaccg aggaagaact ggccttccgt gaggaggcgc
     3061 gccgcttctt ccgcagcgaa atcccgcagt cgatccgcga taaggtcgcg gaaggcgagg
     3121 gcctgacccg cgacgacatg atcaccagcc agcgcatcct gaacgcgcat ggctgggcga
     3181 cgccgaactg gccggtgcag tatggcggca aggattggtc gtcggtgcag gtctatctgt
     3241 accaggacga gatgcagcag gccaacgtgc cgccgccaat cgcgttcaac gtcagcatgg
     3301 tcgggccggt gatcgcgcag ttcggctccg aggaacagaa gcagcgcttc ctgccgaaga
     3361 ccgccaacgc cgacatcttc tggtgccagg gcttctccga acccggcgcc ggttccgacc
     3421 tggcgggcct gcgcacgcgg gccgagcgca agggcgacaa atacgtcatc aacggccaga
     3481 agatctggac cacgctggcg caatacgccg actggatctt ctgcctgtgc cgcaccgatc
     3541 cggcggcgaa gaagcaggaa ggcatcagct tcatcctggt cgatatgaag acgccgggca
     3601 tcaccgtacg gccgatcatc accatcgacg gcggacgcga ggtgaacgag gtgttcttcg
     3661 acaatgtcga agtgccggcg gagaacctgg tcggccagga gaacaa
//
LOCUS       DASSZH010000504         5596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_574458, whole genome shotgun sequence.
ACCESSION   DASSZH010000504 DASSZH010000000
VERSION     DASSZH010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574458"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..101
                     /locus_tag="VFN42_11010"
     CDS             <1..101
                     /locus_tag="VFN42_11010"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423362.1"
                     /note="catalyzes the formation of
                     pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose
                     1-diphosphate from nictinate D-ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HET8997185.1"
                     /translation="APAIAASGVDLLSVGWLTHSATVLDIGLDHVG"
     gene            complement(108..734)
                     /locus_tag="VFN42_11015"
     CDS             complement(108..734)
                     /locus_tag="VFN42_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HET8997186.1"
                     /translation="MKILHLDSSPLGEASASRRLTAAIVDTLRRDLPDAQVSHRDLAA
                     APPAHLSGAVLQAMTSAQPQALPEEQRQERVLTDALLEEFLAADIVVIGVPMYNFSIP
                     TQLKAWIDRIAQAGKTFRYTANGPVGLAGGKRVVIASSRGGVYAGEAAMQALDHQEAY
                     LRIVLGFLGITDIAIVRAEGLALGDAARSKAMVEALAQVDALAERAAA"
     gene            921..1835
                     /locus_tag="VFN42_11020"
     CDS             921..1835
                     /locus_tag="VFN42_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET8997187.1"
                     /translation="MDRLAAMQAFAGVVEHGGFTAAAGALRLSRAMVSKHVQDLEAHL
                     GARLLNRTTRRISLTEAGQLYYQRAARLLADLDETEAAVGALQATPRGRLRINTGMSF
                     GILHLASAVAEYMAAYPNVSVELTLNDRVIDLVDEGYDLAIRITQLADSSLIARRLAS
                     ARAAVCASPAYLERYGWPRHPGELAGHNCLGYSYVQPRDEWRFDGPDGDVAVHVRGTL
                     EANNGDVLRAAALLGLGIARLPTFLIGPDLQRGALLPLMPKYHIPETGIYAVYPHSRH
                     LSAKVRSFVDFLLPRFGEHPPWDAWMQG"
     gene            complement(1848..2528)
                     /locus_tag="VFN42_11025"
     CDS             complement(1848..2528)
                     /locus_tag="VFN42_11025"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HET8997188.1"
                     /translation="MRRSDRLFDIIQRLRTARAPVTAAALAEELEVTPRTVYRDIAAL
                     QGSRVPIEGAAGVGYVLRRGYDLPPLMFTEDELEAVLVGMRLLRRTRDSGLQAAAESV
                     LSKLAAVLPGPLRAQLDAPPFLVSEGQVREPQAVSLAELRVAIREARKLRIVYRDVSG
                     AASSRVIRPVALEYYVEVTLVCAWCELRNDYRHFRADRIEAASVLPDSFAAERERLLS
                     GWVALTRG"
     gene            2602..3018
                     /locus_tag="VFN42_11030"
     CDS             2602..3018
                     /locus_tag="VFN42_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8997189.1"
                     /translation="MITLTDTPDPQLEAVLQDGLASYNAEQAGRRDWRPLAVSAHDPE
                     TGTLCGGLLGRTSMGLFFLDLFYLPRALRGGGLGSAMLRMAEVEATNRGCQAATLVTV
                     NFQAPGFYERHGWEIFGTIPSAPGVERIFMRKALRG"
     gene            3015..4241
                     /locus_tag="VFN42_11035"
     CDS             3015..4241
                     /locus_tag="VFN42_11035"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8997190.1"
                     /translation="MSNAVSLPLEGTNELRRYWPSVAACFASAVFGWGFGFTGTSVYL
                     AELHRLHGWPNALIAGAITEYYLLGALCLTRVHVALRWLGPGRLLATGILLLGLGATL
                     FSRSAQTWQLFLAAAVMACGWASCTSTAISSSLALYFQRQRGLAITLALNGASAAGFT
                     VAPLLLALSQRIGVGNAVPLVALGMAAMVLPLIGLGFGTQPATTDVAHARGGAAMRAG
                     EVLRTWGFWSIALPFALALAAQVGLIVHLVSFLLPRLGAASAATAVALVSVAAMGGRL
                     VLATVIDRLNQRGTAAVSFASQAAGLGLMLAFPHQAEVLYAGCLLFGLSVGNVITFPA
                     LIVQREFPGAAFALVIGLSTAVSQFTFALAPAVLGIIRDATGGYAAVLATCIALQLGA
                     ATLVLCRRETVGAGQD"
     gene            4326..5555
                     /locus_tag="VFN42_11040"
     CDS             4326..5555
                     /locus_tag="VFN42_11040"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HET8997191.1"
                     /translation="MAVKDVKKVVLAYSGGLDTSVILRWLQVTYHCEVVTFTADLGQG
                     EELGPARQKAEMFGVKEIFVDDLREQFVREFVFPMFRANALYEGQYLLGTSIARPLIA
                     QRQIEIAEQVGADAVAHGATGKGNDQVRFELSYYALKPDIKIIAPWREWDLTSRTKLL
                     EFAEQNQIPIAKDKRGEAPFSVDANLLHSSSEGKILEDPAVEADSIVYQRTISPEDAP
                     DKATVISMDFEHGDPVAIDGVRLSPAAVLTRLNELGNANGIGRLDLVENRFVGMKSRG
                     VYETPGGTILLAAHRGIESITLDREAAHLKDQLMPRYAELIYNGFWFSPERRMLQALI
                     DESQKSVSGRVRLKLYKGNATVIGRESPNSLYSMKVVTFEEDQGAYDQFDAQGFIKLN
                     ALRLRLGAMAGRRGGAL"
BASE COUNT          860 a         1931 c         1926 g          879 t
ORIGIN      
        1 ccgcgccggc aatcgccgcc agcggcgtgg acctgctgtc agtggggtgg ctgacccaca
       61 gcgccacggt gctggacatc ggcctcgatc acgtggggtg aatccgctca ggccgcggcg
      121 cgttcagcca acgcgtcgac ctgggccagc gcctccacca ttgccttgct tcgggccgca
      181 tcgcccaagg ccaggccctc ggcgcgcacg atcgcaatat cggtgatgcc gaggaagccg
      241 agcacgatgc gcagatacgc ctcctggtgg tcgagcgcct gcatcgccgc ttcgcccgca
      301 tacacgccgc cacgcgacga cgcgatcacc acgcgtttgc cgccggccag cccgaccggg
      361 ccgttcgcag tgtagcggaa ggtcttgccc gcctgcgcga tgcgatcgat ccacgccttg
      421 agctgggtcg gaatggagaa gttgtacatc ggcacgccga tcaccacgat atcggccgcc
      481 aggaactcct ccagcaaggc gtcggtcagc acgcgctcct ggcgctgctc ttccggcagc
      541 gcctgcggct gggcgctggt catcgcctgc agcaccgcgc cggacaggtg cgccggcggc
      601 gccgccgcga ggtcgcgatg cgagacctgg gcgtccggca ggtcccggcg cagggtatcg
      661 acgatcgccg cggtaagccg ccgcgaggcg gaggcctcgc caagtgggct ggaatcgagg
      721 tgcaggattt tcatggctct ctgtcccggc tggttgcggc cggccctgcc aattcggctg
      781 ccgacccgtt gtggtccggc aggcttgccg ccttaccggt gcgcccagga ggtaagccgt
      841 cacccagggt ggataaaccg gcctctgatt gacagattgt ctactgttga tgcccaatct
      901 tccggcaaag gagcagcatc atggaccggc tggcggcgat gcaggccttc gcgggcgtgg
      961 tggaacacgg cgggttcacc gccgccgccg gcgcgctgcg cctgtcgcgc gccatggtca
     1021 gcaagcacgt gcaggacctg gaggcgcatc tcggcgcccg cctgctgaac cggaccaccc
     1081 gccggatcag cctgaccgag gccggccagc tgtattacca gcgcgccgcc cgtcttctcg
     1141 ccgacctgga cgagaccgag gccgccgtgg gcgccttgca ggccacgccg cgcgggcggc
     1201 tgcggatcaa cacgggcatg agcttcggca tcctgcatct ggccagcgcg gtggcggaat
     1261 acatggccgc ctatcccaac gtctccgtcg agctgacgct gaacgaccgg gtcatcgacc
     1321 tggtcgacga aggctacgac ctcgccatcc gcatcacgca gcttgccgac agcagcctga
     1381 tcgcccgccg cctggcatcg gcccgcgcag cggtctgcgc gtcgccggcc tacctggaac
     1441 ggtacggctg gccgcgccat cccggcgaac tcgccggcca caattgcctc ggctacagct
     1501 atgtccagcc acgcgacgaa tggcgtttcg acggtcccga cggcgacgtc gccgtgcatg
     1561 tgcgcggcac gctggaagcc aacaacggcg acgtactgcg tgccgcggcg ctgctgggcc
     1621 tgggcatcgc tcggctgcca accttcctga tcggtcccga tctgcagcgc ggcgcgttgc
     1681 tgccgctgat gccgaaatac cacatcccgg aaaccgggat ttatgcggta tatccgcaca
     1741 gccggcacct ctcggccaag gtgcgcagtt tcgtggattt cctgctgccc cgcttcggcg
     1801 agcacccgcc ctgggatgcc tggatgcagg gctgaccggc ggggcagtca gccccgggtc
     1861 agcgccaccc agcctgacag caggcgttcc cgctcggcgg cgaaactgtc gggcagcacc
     1921 gacgcggcct cgatccggtc ggcgcggaaa tgccggtagt cattgcgcag ctcgcaccag
     1981 gcgcagacca gcgtgacctc gacatagtat tccagcgcca cggggcggat cacccgcgaa
     2041 ctcgccgcac cgctgacgtc gcggtacaca atccgcagct tgcgcgcctc ccggatcgcc
     2101 acgcgcagct cggccaggct gaccgcctgc ggttcgcgca cctggccctc cgacaccagg
     2161 aagggcggcg cgtcgagctg cgcccgcaac ggtccgggca ggaccgccgc cagcttggac
     2221 agcaccgact ccgcggcggc ctgcagcccg ctgtcccggg tgcgccgcag caggcgcatg
     2281 cccaccagca ccgcttccag ctcgtcctcg gtgaacatca gcggcggcag gtcatagcca
     2341 cggcggagca cgtagcccac gccggcggcg ccttcgatcg gcacgcggct gccctgcagg
     2401 gccgcgatgt cgcggtacac cgtgcgcggc gtcacctcca gctcctcggc caaggcggcc
     2461 gcggtcaccg gcgcgcgggc cgtgcgcagc cgctggatga tgtcgaacaa ccggtcggac
     2521 cgccgcatgg gcagagacta ctgacaaaac gctgtcagtg gccatccggc agactacgcc
     2581 cgtcaaccgg aggagccagg catgatcacc ctgacggaca cgcccgaccc gcaactggaa
     2641 gccgtgctgc aggacgggct ggccagctac aacgccgagc aggcgggccg gcgcgactgg
     2701 cgcccgctgg cggtcagcgc gcatgatccc gaaacaggca cgctgtgcgg cgggctgctc
     2761 ggccgcacct ccatgggcct gttcttcctg gacctgttct acctgccgcg ggccttgcgc
     2821 ggcggcggcc tgggcagcgc gatgctgcgc atggcggaag tggaagccac caaccgcggc
     2881 tgccaggcgg ccacgctggt cacggtcaac ttccaggcac ccgggttcta tgagcggcat
     2941 ggctgggaga ttttcggcac catccccagc gctccgggcg tcgaacgcat cttcatgcgc
     3001 aaggcgctga ggggatgagc aacgccgtct ccctgccgct ggagggtacg aacgagcttc
     3061 ggcggtactg gccaagcgtc gcggcctgct tcgccagcgc ggtgttcggc tggggcttcg
     3121 gcttcaccgg cacctcggtc tacctggcgg aactgcaccg actgcacggc tggccgaacg
     3181 cgctgatcgc cggcgccatc accgaatact acctgctggg tgcgctctgc ctgacccgcg
     3241 tgcatgtcgc gttgcgctgg ctcggccccg gacggctgct ggcgaccggc atcctgctgc
     3301 tgggtcttgg cgcgacgctg ttcagccgca gcgcgcagac ctggcagctg ttcctcgccg
     3361 ccgcggtgat ggcgtgcggc tgggcgagct gcacctccac cgcgatctcg tcgtcgctgg
     3421 cgctgtattt ccagcggcag cgcgggctgg ccatcacact ggcactgaac ggcgccagcg
     3481 cggccggctt caccgtcgcg ccgctgttgc tggcattgag ccagcggatc ggcgtgggca
     3541 acgcggtgcc gctggttgcc ctggggatgg cggctatggt cttgccgttg atcggcctgg
     3601 gattcggtac gcaacctgca acgacggacg ttgcgcacgc acgtggcgga gcggcgatgc
     3661 gtgcgggcga ggtgctgcgg acctgggggt tctggtcgat cgccctgccc ttcgccctgg
     3721 cgctggccgc gcaagtgggc ctgatcgtgc accttgtctc cttcctgctg ccgcggctcg
     3781 gcgccgccag cgcggccacc gcggtggcgc tggtgtccgt cgcggcaatg ggcggacgcc
     3841 tggtgctcgc cacggtgatc gatcggctga accagcgcgg aacggcggcg gtcagctttg
     3901 ccagccaggc ggcggggctg ggcctgatgc tcgccttccc gcaccaggcg gaggtgcttt
     3961 acgcgggctg cctgctgttc ggcctgtcgg tgggcaacgt gatcaccttc ccggctctga
     4021 tcgtgcagcg cgagttcccc ggtgcagcgt ttgccctggt gatcgggctc agcacggcgg
     4081 tcagtcagtt cacctttgcc ctggcgccgg cggtgcttgg catcatccga gacgcgaccg
     4141 gtggctacgc ggcggtgctg gccacctgca tcgccctgca gctcggcgcg gcgacactgg
     4201 ttctgtgccg acgggaaacg gtcggggcgg gtcaggactg accggctcct gggtgtcgcc
     4261 cgccgcattg cctccccccg ccccaggcgc tactcccggc ccgcagtgaa aaaggaccgc
     4321 ccaccatggc cgtgaaggat gtgaaaaagg tcgtgctcgc ctattccggc gggctggaca
     4381 ccagcgtgat cctgcgctgg ctgcaggtca cctatcattg cgaggtggtc accttcaccg
     4441 ccgacctcgg ccagggcgag gagctcggcc cggcacggca gaaggccgag atgttcgggg
     4501 tgaaggaaat cttcgtggac gacctgcgcg aacagttcgt ccgcgagttc gtcttcccga
     4561 tgttccgcgc caatgcgctg tatgagggcc agtacctgct gggcacctcg atcgcccgcc
     4621 cgctgatcgc acagcggcag atcgagatcg ccgaacaggt tggcgccgac gcggtggcac
     4681 acggcgcgac cggcaagggc aacgaccagg tgcggttcga gctcagttat tacgcgctga
     4741 agccggacat caagatcatc gcgccgtggc gcgaatggga cctgaccagc cgtacgaaac
     4801 tgctggagtt cgccgagcag aatcagattc cgatcgccaa ggacaagcgc ggcgaggcgc
     4861 cgttcagcgt ggacgccaac ctgctgcact catcgtcgga aggaaagatc ctggaagatc
     4921 cggcggtgga ggcggattcc atcgtgtatc agcgcaccat cagcccggag gatgcgccgg
     4981 acaaagcgac ggtgatctcg atggatttcg agcacggcga tccggtggcg atcgatggcg
     5041 tacgcctgtc gccggctgcg gtgctaacga ggctgaacga gctgggcaac gccaatggca
     5101 tcggccggct ggacctggtg gagaaccgct tcgtcggcat gaagtcgcgc ggcgtgtacg
     5161 aaacgccggg cggcaccatc ctgctggcgg cgcatcgcgg catcgagagc atcacgctgg
     5221 accgcgaggc ggcgcatctg aaggaccagc tgatgccgcg ctacgcggag ctgatctaca
     5281 acggcttctg gttcagcccg gagcggcgca tgctgcaggc actgatcgac gagagccaga
     5341 agagcgtcag cggccgggtg cggctgaagc tgtacaaggg caatgccacg gtgatcggcc
     5401 gcgaatcgcc gaacagcctg tactcgatga aggtggtcac gttcgaggag gaccagggcg
     5461 cctacgacca gttcgacgcg cagggcttca tcaagctgaa cgcgctgcgc ctgcggctgg
     5521 gcgcgatggc aggacggcgc ggcggcgcgc tgtagaccgc gccagattcc tccccctccc
     5581 cttgcgggag ggggtt
//
LOCUS       DASSZH010000505         3492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_369930, whole genome shotgun sequence.
ACCESSION   DASSZH010000505 DASSZH010000000
VERSION     DASSZH010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369930"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VFN42_11045"
     CDS             complement(<1..1041)
                     /locus_tag="VFN42_11045"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HET8997192.1"
                     /translation="MQGILDAILKRLITVGHLSVRWPDGSSKEYGTGDRPAAAMAIRT
                     QSAVRQLVRNPALTFGECYMDGTVTAEGCTIYDLLDLLVRNLTVGGNNHPGARLRRVV
                     GHVRRRLDQYNPARKAQRNVAHHYDLNGRLYSLFLDRDRQYSCAYFPRGNETLEEAQI
                     AKKRHIAAKLLLDRPDLTVLDIGCGWGGLALTLARDYGARVLGITLSQEQLAEARSRA
                     AAEGLQDRVQFELRDYRAVDQRFDRIVSVGMFEHVGVNHYRSFFDCVSRCLDPQGVAL
                     LHAIGRSDGPGFTNPWIAKYIFPGGYCPSLSEVIPVIERTGLVATDIEILRLHYAETL
                     RHWRRRFAANRDA"
     gene            complement(1253..1828)
                     /gene="rplI"
                     /locus_tag="VFN42_11050"
     CDS             complement(1253..1828)
                     /gene="rplI"
                     /locus_tag="VFN42_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006116000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET8997193.1"
                     /translation="MAAVELILLQRVENLGQMGDVVKVKPGYARNFLLPEKKALRATK
                     DNMARFEQQRAQLEAQNLKRREEAERVAERVHGISVVIIRQAGESGSLYGSVSGRDVA
                     EATTEAGLSITRQQVILEQPIKMLGLSQVRVVLHPEVSIPVTVNVARSAEEAERQARG
                     EAVGAAAEEPEDVLTEENLFESDVPPEPQAA"
     gene            complement(1842..2117)
                     /gene="rpsR"
                     /locus_tag="VFN42_11055"
     CDS             complement(1842..2117)
                     /gene="rpsR"
                     /locus_tag="VFN42_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003622836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET8997194.1"
                     /translation="MSDSTEVNPAARRAAVGARRPFYRRRKSCPFSGPNAPKIDYKDV
                     RLLSRFLSERGKIVPSRITAVSAKKQRELALAIKRARFLALLPYTLS"
     gene            complement(2124..2588)
                     /gene="rpsF"
                     /locus_tag="VFN42_11060"
     CDS             complement(2124..2588)
                     /gene="rpsF"
                     /locus_tag="VFN42_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET8997195.1"
                     /translation="MPLYECVMIARNDVTQQQVEAIADGIASQLEAESGAVKKREYWG
                     LRSLAYRIKKNRKGHYMLLGLDAQPTFINEMERQLGLNEDVLRFMTLRVEEIEEAPSA
                     ILSRKSDDRERSFRGPKPAGRFESGRRRGYDDREEFRARDEVREDVRPGGEE"
     gene            2818..>3492
                     /locus_tag="VFN42_11065"
     CDS             2818..>3492
                     /locus_tag="VFN42_11065"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8997196.1"
                     /translation="MAPNTKRVFYVKYLSSPLYAQILSQRPDVQLDRLENESPDDVAT
                     PILSAAHVYQIGASRQVIAPHFHASPALLDRMPNLVAVSSNGAGYDTVDVEACTRAGI
                     AVVNQSGGNKEGVAEHALAMMLTLSKRIIETDRRMRAGPGIPRNAYIGRELLNRTVGI
                     IGIGNVGGRVAQLCGGLFAMRVLAYDPHLSAEQIKARGAEKVELDELLRQSDFVSVHC
                     PLDAGSR"
BASE COUNT          522 a         1184 c         1190 g          596 t
ORIGIN      
        1 cgcgtcgcga ttggcggcga agcggcggcg ccagtggcgc agcgtctcgg catagtgcag
       61 ccgcaggatc tcgatgtcgg tggcgaccag gccggtgcgc tcgatcaccg ggatcacttc
      121 ggacagcgac gggcagtagc caccggggaa gatgtatttg gcgatccagg gattggtgaa
      181 tcccggcccg tcgctgcggc cgatcgcgtg cagcagcgcc acgccctgcg gatccaggca
      241 gcgcgacacg cagtcgaaga acgagcggta gtggttcact ccgacatgct cgaacatgcc
      301 gaccgagacg atccggtcga agcgctggtc cacggcccgg taatcgcgca gctcgaactg
      361 cacgcggtcc tgcagcccct cggccgcggc tcggctgcgg gcttcggcca gctgttcctg
      421 cgacagggtg atgcccagca cgcgggcgcc gtaatcgcgt gccagggtca gcgccaagcc
      481 accccagcca cagccgatgt ccagcaccgt caggtccggc cggtccagca gcagcttggc
      541 ggcgatgtgg cgcttcttgg cgatctgcgc ttcctccagc gtctcgttgc cgcgggggaa
      601 ataggcgcag gagtattgcc ggtcgcggtc caggaacagg ctgtacagcc ggccattgag
      661 gtcgtaatgg tgggcgacgt tgcgttgcgc cttgcgggcc ggattgtact ggtccagccg
      721 ccggcgcaca tggccgacca ccctgcgcaa ccgtgcaccc gggtgattgt tgccgccgac
      781 cgtcagattg cggaccagca gatccagcag gtcgtagatg gtgcagcctt ccgccgtcac
      841 cgtgccatcc atgtagcact cgccgaaggt cagggctgga ttgcgcacca attggcggac
      901 cgcgctctgg gtgcggatgg ccatcgccgc agcgggccgg tcgccggtcc cgtattcctt
      961 cgagcttcca tccggccagc gcacgctcag atggccgact gtgatcaacc gcttcaggat
     1021 tgcgtcgagg atcccctgca tcgcgccgcg ccctctctcc tttgatgatc cagagatagg
     1081 cgtacattgg cgaacgtgcg gcctgacgtc aaaacgaact tccgcaaaac cctgtgttcc
     1141 gggcggagcg gcaggcggaa tttttccggt gtccggacgg ttcgccgggc gcaatgcgcg
     1201 cggccggacc gccccctccg cggtgcagag ggggcgtcac tggttgcggt ggtcaggctg
     1261 cctgcggttc cgggggaacg tcggactcga acaggttttc ctcggtcagc acgtcttccg
     1321 gctcttcggc cgccgcgccc accgcctcgc cgcgtgcctg gcgctctgcc tcctcggcgc
     1381 tgcgggcgac gttgacggtg acagggatgc tgacctccgg atgcagcacc acgcggacct
     1441 gggacaggcc cagcatcttg atcggctgct ccaggatcac ctgctggcgg gtgatggaca
     1501 ggccggcctc ggtcgtcgcc tcggccacgt cccgtccgga gaccgagccg tacaggctgc
     1561 ccgactcgcc cgcctggcgg atgatcacca cggagatgcc gtgcacccgt tcggcaacgc
     1621 gttcggcttc ctcgcggcgc ttgaggttct gcgcctccaa ctgggcgcgc tgctgttcga
     1681 agcgggccat gttgtctttg gtggcgcgta gcgccttctt ctcgggcagc aggaagttgc
     1741 gggcatagcc cggcttcacc ttcaccacat cgcccatctg ccccaggttt tccacccgct
     1801 ggagcaggat cagttccacg gcagccatgg ttccggcctc ctcagctcag ggtgtagggc
     1861 agcagcgcca ggaagcgggc gcgcttgatg gccagggcca gttcgcgctg cttcttggcc
     1921 gacacggcgg tgatgcggct gggcacgatc ttgccgcgct ccgacaggaa gcgggacagc
     1981 aggcgcacgt ccttgtagtc gatcttcggc gcgttggggc cggagaacgg gcaggacttg
     2041 cggcggcggt agaacgggcg gcgggcgccg accgcggcgc ggcgggccgc gggattgact
     2101 tcagtgctat cggacatgct ggcttactcc tcgccgccgg ggcggacgtc ttcgcggacc
     2161 tcgtcgcggg cgcggaattc ctcgcggtcg tcatagccgc ggcggcggcc gctctcgaac
     2221 cggccggccg gcttcggacc gcggaagctg cgctcccggt catccgactt gcgcgacagg
     2281 atagccgagg gggcctcctc gatctcctcg acccgcaggg tcatgaagcg cagcacgtct
     2341 tcgttgaggc cgagctggcg ctccatctcg ttgatgaagg tcggctgggc gtccaggccg
     2401 agcagcatgt aatggccctt gcggttcttc ttgatccggt aggccaggct gcgaaggccc
     2461 cagtattcgc gcttcttgac ggcgccgctc tcggcttcca gctggctggc gatgccgtcg
     2521 gcgatcgcct cgacctgctg ctgcgttacg tcattgcgtg cgatcatcac gcattcatac
     2581 aatggcatct gtggctccct ctggctggtg gccctggccg ggatggccac gcccggaacc
     2641 gggcgctggg catagccgga gcggatgccg cccgccccgg cagggaaggc gcggcgtaaa
     2701 gcacggtttg cccggcggtg caaccgcggg ccgccgggac ctgccgctat ggcgagtgcg
     2761 ggtcgccaac cctgctccgc gtcattatcc tcccgtgacc aaacaggagg aagccgcatg
     2821 gcgccgaaca ccaagcgcgt gttctacgtg aaatacctgt cctcgccgct ctatgcgcag
     2881 atcctgtcgc agcggccgga cgtgcagctc gaccggctgg agaacgaaag cccggacgac
     2941 gtcgccacac ccatcctgtc cgccgcgcat gtgtatcaga tcggcgcctc ccgccaggtg
     3001 atcgcgccgc acttccacgc cagccccgcc ttgctggacc gcatgccgaa cctggtggcg
     3061 gtgtcgtcca acggcgccgg ctacgacacg gtggacgtcg aggcctgcac ccgcgccggt
     3121 atcgccgtgg tcaaccagtc cggcggcaac aaggaaggcg tggcggagca cgcgctggcc
     3181 atgatgctga ccctgtccaa gcgcatcatc gagaccgatc gccggatgcg cgccggcccg
     3241 ggcatcccgc gcaacgccta tatcggccgc gagctgctga accgcaccgt cggcatcatc
     3301 ggcatcggca atgtcggcgg ccgcgtggcg cagctatgcg gcgggctgtt cgccatgcgg
     3361 gtgctggcct acgacccgca cctcagcgcg gagcagatca aggcgcgcgg cgcggagaag
     3421 gtggaactcg acgaactgtt gcgccaatcg gatttcgtct cggtgcactg cccgctcgac
     3481 gccggatcgc gc
//
LOCUS       DASSZH010000506         3239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1103329, whole genome shotgun sequence.
ACCESSION   DASSZH010000506 DASSZH010000000
VERSION     DASSZH010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103329"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="VFN42_11070"
     CDS             <1..1088
                     /locus_tag="VFN42_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HET8997197.1"
                     /translation="YIALQLVGLEMVARILGLSGELPLLIAFLALALYTYVSGLRAPA
                     LIAFIKDLMVYIVVLAAVAVIPVRLGGYAAMFHMAGVALAQHRAGGLLLAPPQVVPYA
                     TLAFGSALAAFLYPHTLTGVFAARSADTIRRNAILLPAYTLLLGMIALLGYMALAAGI
                     RVTQPNDAVPALLQTMFPGWFAGFALAAIAVGALVPATVMSIGAASLVSRNVWKAFFR
                     PDATPREETEVARIVSLAVKTGALLFVVLLPVKFSIYLQLLGGLWILQTLPAVLLGLA
                     AFRLRARALLAGWFAGTVWGTWTVFADHWVPVHQIHLAGASYGIYTGLLALAVNLVAA
                     AAVNLMLARSEQLQGGDLAAPAGAEGP"
     gene            1169..2428
                     /locus_tag="VFN42_11075"
     CDS             1169..2428
                     /locus_tag="VFN42_11075"
                     /inference="COORDINATES: protein motif:HMM:NF015078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8997198.1"
                     /translation="MPDKPDPAVRRLMLFFAVVYTVEGIGQAKVGVVWQPLTWFLKQV
                     HGWTALQVSAGLAVLDLPWIVKPLYGAISDFLPLFGYRRRSYLLLANAAAVAAFVWVA
                     QERAPAAIVFALLLTSVAMAVSSTLCGALLVENGQRYGSNGAFVNQQWLWFNVALVLA
                     SLLGGALVQLLPPAGALHAAALAAAVAPLAVLAGLALVHEQPAKLDPAALKRTAGAFV
                     AAFRTRTLWLVAGFLFCYYFSPGFGTPLYFTMTDRLGFSQAFIGALSSVNALGWIAGG
                     LLYRWALVRMPTGPLLRLSIACGVASTLAYLGLVDPASAVLVYFAGGVAGMIANIATL
                     TLAAEHCPAGAEGFAFAGLMSVINLATPLSDTTGSFLYEHVFHHALAPLIVLSAAFTG
                     FVLVLMRLFGIARDTAAAEAIPGPSGE"
     gene            2582..>3239
                     /locus_tag="VFN42_11080"
     CDS             2582..>3239
                     /locus_tag="VFN42_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HET8997199.1"
                     /translation="MSSDAHGATIDHHAPAFESSGLKQPYHLVDPSPWPIVGALGGLC
                     TVLGIIFVAHFGSWTLLVIGAVIVLFTMFMWWRDVIRESRTPGLHSPIVRLGLRYGMM
                     LFIASEVMFFVGFFWAYFNFLIFPETQGNGQVVWPPSNIHSFDPFSLPFLNTMILLLS
                     GCTITWAHHALLENKRKDLLTGLGLTIILGLCFTCGQIAEYSDAPFKFVGGGVYPSTF
                     F"
BASE COUNT          400 a         1087 c         1111 g          641 t
ORIGIN      
        1 cctatatcgc cttgcaactg gtcgggctgg agatggtcgc ccgcatcctg gggctgtccg
       61 gcgagctgcc gctgctgatc gcgttcctgg cgctggcgct gtacacgtat gtgtccggac
      121 tgcgggcgcc ggcgctgatc gccttcatca aggacctgat ggtctatatc gtggtgctgg
      181 cggcggtggc ggtgatcccg gtgcggctgg gcggctacgc ggcgatgttc cacatggccg
      241 gcgtggcctt ggcgcagcat cgcgcgggcg ggctgctgct ggcccctccg caggtggttc
      301 cctacgcgac actcgcattc ggctccgcgc tcgccgcctt cctgtatccg catactctca
      361 cgggcgtgtt cgctgcccgc agcgccgata ccatccgccg caatgccatc ctgctgccgg
      421 cctacacgct gctgctgggg atgatcgcgc tgctcggcta catggcgctg gccgcgggga
      481 tccgggtcac ccagccgaac gatgcggtgc ccgcgctgct gcagacgatg ttcccggggt
      541 ggttcgccgg attcgcgctg gccgccatcg cggtgggcgc attggtgccg gctaccgtca
      601 tgtcgatcgg tgccgccagc ctggtgtcgc gcaacgtctg gaaggcgttc ttccggccgg
      661 acgccacgcc gcgggaggag accgaggtag cccggatcgt ctccctggcg gtgaagaccg
      721 gcgcgctgct gttcgtggtg ctgctgccgg tgaagttctc catttacctg cagttgctgg
      781 gcgggctgtg gatcctgcag acgctgccgg cggtgctgct gggactggcg gcgttccggc
      841 tgcgcgcccg ggcactgctg gcgggctggt ttgccggtac ggtctggggc acctggacgg
      901 tgttcgcgga tcactgggtg ccggtgcacc agatccacct ggccggggcc agctacggca
      961 tctacaccgg actgttggcg ctggccgtga acctggtcgc ggcggccgcg gtgaacctga
     1021 tgctggcccg gtcggagcag cttcagggcg gcgacctcgc cgcgccggcg ggtgccgaag
     1081 gtccctgacc ggcacacacc tgcccggcgc acaattggcc agtttgcgtc tggcgggata
     1141 tacccaggcg gccacaagcg gcccgcgcat gccggataaa cccgatcccg ccgtacggcg
     1201 gctcatgctg ttcttcgcgg tggtctacac cgtggaaggc attggccagg ccaaggtcgg
     1261 tgtggtctgg cagccgctga cctggttcct gaaacaggtg catggctgga ccgcgctgca
     1321 ggtgtcggcc gggctggcgg tgctcgacct gccctggatc gtcaagccgc tgtacggcgc
     1381 gatctccgac ttcctgccgc tgttcggcta ccgccggcgc agctacctgc tgctggccaa
     1441 tgccgctgcc gtggccgcct tcgtctgggt ggcgcaggaa cgcgccccgg cggccattgt
     1501 cttcgccctg ctgctgacct cggtcgcgat ggcggtgtcg agcacgctgt gcggggcgct
     1561 gctggtggag aatggccagc gctacggcag caacggcgcc ttcgtgaacc agcaatggct
     1621 gtggttcaac gttgcgctgg tgctggcctc gctgctgggc ggcgcgctgg tgcagctgct
     1681 gcccccggcc ggagccttgc atgccgcggc gctcgccgcc gcggtggcgc cgctcgcggt
     1741 gctggcaggt ctggcgctgg tgcacgagca gccggcgaaa ctcgatccgg cggcgctgaa
     1801 gcggaccgcc ggcgcgttcg tcgcggcctt ccgcacccgg accctgtggc tcgtggcggg
     1861 attcctgttc tgctactatt tcagtcccgg cttcggcacg ccgctgtatt tcaccatgac
     1921 cgaccggctg gggttctcgc aggcgttcat cggcgcgctg tcctcggtca atgccttggg
     1981 ctggatcgcc ggcggcctgc tctaccgctg ggcgctggtg cgcatgccga ccgggccgct
     2041 gctgcggctg tcgatcgcgt gcggcgtggc cagcacgctg gcctatctgg gcctggtcga
     2101 tccggccagc gcggttctgg tgtatttcgc cggcggcgtg gcggggatga tcgccaacat
     2161 cgccaccctg acgctggcgg ccgaacattg tccggccggc gcggaaggtt tcgccttcgc
     2221 cgggctgatg tcggtgatca atctggcgac tccgctatcc gacaccaccg ggtctttcct
     2281 gtacgagcac gtgttccacc acgcgctggc gccgctgatc gtgctgtccg cggcgttcac
     2341 cggattcgtg ctggtgctga tgcggctgtt cggcatcgcg cgcgacaccg ccgcggcaga
     2401 ggcgattccc ggaccgtcgg gcgaatgacg gcgcgccgca gggctgtgca caaaaccatt
     2461 gtacgtaagg caattcagcc cttttcatgc acccgcctct tgaactggtg gccgaattcg
     2521 tgatgatgcc cccaaccgcg ccgggcggac tccccccggc tgccttgggg acggacgacg
     2581 tatgagcagc gacgcacacg gcgccacgat cgaccaccac gcgccggcct ttgagagttc
     2641 cggactgaaa cagccttatc acctggtcga tcccagccca tggccgatcg tcggcgcatt
     2701 gggcggcctc tgcaccgtgc tgggcatcat cttcgtcgcg catttcggca gttggacgct
     2761 gctggtgatc ggcgccgtga tcgtgctgtt caccatgttc atgtggtggc gcgacgtgat
     2821 ccgggagagc cgcacgcccg gcctgcactc gccgatcgtc cggcttggcc tgcgctatgg
     2881 catgatgctg ttcatcgcca gcgaggtgat gttcttcgtc ggcttcttct gggcgtattt
     2941 caacttcctg atcttcccgg agacgcaggg caacgggcag gtcgtctggc cgcccagcaa
     3001 catccattcg ttcgacccgt tcagcctgcc gttcctcaac acgatgatcc tgctgctgtc
     3061 cggctgcacc atcacctggg cgcaccacgc gctgctggag aacaagcgga aggatctgct
     3121 gaccgggctc ggtctgacca tcatcctggg gctctgcttc acctgcggcc agatcgcgga
     3181 atattccgac gcgccgttca agttcgtcgg cggcggcgtg tatccctcga ccttcttcc
//
LOCUS       DASSZH010000507         3506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_148296, whole genome shotgun sequence.
ACCESSION   DASSZH010000507 DASSZH010000000
VERSION     DASSZH010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148296"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1218)
                     /gene="miaB"
                     /locus_tag="VFN42_11085"
     CDS             complement(<1..1218)
                     /gene="miaB"
                     /locus_tag="VFN42_11085"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007282253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HET8997200.1"
                     /translation="MTPSEPTANSSKRLHVITWGCQMNVYDSARMADVLAPLGYAPVG
                     QPDDADMVILNTCHIREKAAEKVFSELGRLRRLKEARAADGSRMILAVAGCVAQAEGE
                     EILARAPFVDIVLGPQTYHRLPEMVARASRAAGAVIDTDFPAEQKFDFLPEAAAPQGV
                     CAFLSIQEGCDRFCSFCVVPYTRGAEQSRPAAAVLAEARRLVAQGAREITLLGQNVNA
                     WHGEAPDGGEWDLGRLLRELAEIPGLLRLRYATSHPRDMTASLIAAHRDVRLLMPFLH
                     LPVQSGSDRILAAMNRRHRAADYLRIVEKLRTARPDLALSSDFIVGHPGETEADFQAT
                     LDLVRQVGFAQAFSFRYSARPGTPAAAAPGQVPEDEKERRLQELQALLRDQQARFNAG
                     CVGTALPVLFTGPG"
     gene            complement(1508..2272)
                     /locus_tag="VFN42_11090"
     CDS             complement(1508..2272)
                     /locus_tag="VFN42_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET8997201.1"
                     /translation="MSTSQQSFAAAQYSVRAQDYVTSAVHSSGADLDQIEAHVRGHAT
                     ARVLDLGCGGGHVSYRAAPHVAAVVACDVTQDMLDAVARTAAERGLDNISVRQAAAED
                     LPFADASFDMVLCRFTVHHWQSMEAGLRQARRVLKPEHTALFIDTVAAAEPVFDTHLQ
                     AVELLRDVSHVRNYSTAEWVAALARAGFRLDGITQRRLRMEFPVWTARTRTPEAHVAA
                     IRSLQRGAPAQVQRHFAIDDDGSFDIETVTFLVRAV"
     gene            complement(2269..>3506)
                     /gene="selD"
                     /locus_tag="VFN42_11095"
     CDS             complement(2269..>3506)
                     /gene="selD"
                     /locus_tag="VFN42_11095"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HET8997202.1"
                     /translation="ALVILGLGDARAVAWRNGLSVSGRLIWQWKDYLDRRWIGMYQKP
                     MRRMDSDDPMRCGGCGAKVGAEVLAGALAALPQMPGADVLVGLDAADDAAVLLPPAGQ
                     AIVQSVDHFRAFIDDPFLFGQIAAAHALSDLHAMGARPWSALAIAGVPYSSGDRMRAD
                     LAAMLLGAAETLKADGCTLVGGHSAEAAEAALGFAVTGLADPKRLLRKSGLRLGDALV
                     LTKRLGTGVILAGHMRGEAKAAWLAAALDSMRVSNAAAVQVLRDHGVTACTDVTGFGL
                     AGHLLEMLRASKVAAVLWPDAVPALPGAAALAARGIESTLAPDNRRMLGPLGMQPDLA
                     LLVDPQTSGGLLAGVPVEQAEACLAALHAAGSPAAIVGRVVQPASRTAFPLRLDRSDA
                     KAGQIRSGQGSIGRRGDPG"
BASE COUNT          545 a         1244 c         1230 g          487 t
ORIGIN      
        1 gcccggcccg gtgaacagca ccggcagcgc cgtgccgacg cagccggcgt tgaaccgggc
       61 ctgctggtcg cgcagcaacg cctgaagttc ctgcagccgg cgctccttct catcctccgg
      121 cacctggccg ggcgcagcgg ctgcgggcgt gccggggcgg gccgaatagc ggaagctgaa
      181 ggcctgggcg aagccaacct gccggaccag gtccagtgtg gcctggaaat cggcctcggt
      241 ttcgcccgga tggccgacga tgaagtccga ggacagcgcg aggtcggggc gggcggtgcg
      301 cagcttctca acaatacgca gatagtcggc ggcgcggtgc cggcggttca tcgccgccag
      361 gatgcggtct gagccggatt gcaccggcaa atgcaggaag ggcatcagca gacgcacgtc
      421 ccgatgtgcg gcgatcagcg aggcggtcat gtctcgcgga tgcgacgttg cgtagcgcag
      481 ccgcagcagg cccgggattt ccgccaactc gcgcagcagg cggccgaggt cccattcgcc
      541 gccgtccggc gcctcgccgt gccaggcatt cacgttctgg ccgagcaggg tgatctcgcg
      601 ggcgccttgc gccaccagcc tgcgggcttc ggccagaacg gcagcagcag gacggctctg
      661 ttcggcgccg cgggtgtagg gcacgacgca gaagctgcag aaccggtcgc agccttcctg
      721 gatgctgagg aaggcgcaca cgccctgtgg cgcggcggct tccggcagga aatcgaattt
      781 ctgttcggcg ggaaaatcgg tgtcgatcac ggcgccggcg gctcgggagg cgcgggccac
      841 catctcgggc aggcggtggt aggtctgcgg gccaagcacg atatcgacga agggggcacg
      901 ggcaaggatc tcctcgcctt ccgcctgggc gacgcagccg gccaccgcca ggatcatgcg
      961 gctgccgtcg gcggcgcggg cttccttcag ccggcgtagc cggccgagtt cggaaaagac
     1021 cttctccgcc gccttctcgc ggatatggca ggtattcagg atcaccatgt cggcgtcgtc
     1081 cggctggccc accggcgcgt agccaagcgg cgccagcacg tcggccatac gggcgctgtc
     1141 atacacgttc atctggcaac cccaggtgat gacgtgcagc cgcttgctgg aattggcggt
     1201 gggttcgcta ggcgtcatag ggcaggctcc gctccgcacg cccggaacag cccggcgtgg
     1261 gaagcggcag caatggtggc ccgaatcggc ggggtcaagc gcagcgccaa caaacctccc
     1321 tggactggcg ccagtctgcc gcgaaccgga agcctgctgt caagcgaaag gactgacata
     1381 atctcctcct cccttttggg aggcggttgg gcggaggagt cgcgggttgc catcccaata
     1441 tcgcgcctga acgctcgacc cctcccccat ccccctcccg caaggggagg gggaggagta
     1501 cggcctccta taccgcgcgc accaggaagg tgactgtctc gatatcgaag ctgccgtcgt
     1561 cgtcgatggc gaagtgccgc tgcacctggg cgggtgcccc gcgctggagg gaacggatgg
     1621 cggcgacatg cgcctccggg gtgcgggtgc gcgcggtcca caccggaaac tccatgcgca
     1681 ggcggcgctg ggtgatgccg tccaggcgga agccggcccg ggccagcgcc gccacccact
     1741 ccgccgtgct gtagttgcgc acgtgcgaca cgtcgcgcag cagttccacc gcctgcagat
     1801 gcgtgtcgaa gaccggttcg gccgcggcga ccgtatcgat gaacagcgcg gtatgctccg
     1861 gcttcagtac ccggcgggcc tgccgcaggc cggcctccat gctctgccag tggtgcaccg
     1921 tgaagcggca cagcaccatg tcgaagctgg catcggcaaa gggcaggtcc tccgcggccg
     1981 cctggcgaac ggagatgttg tccagaccgc gttccgctgc ggtcctggcc accgcgtcca
     2041 gcatgtcctg ggtgacgtcg caggcgacca ccgcggcgac atgcggcgcc gcgcggtagc
     2101 tgacatgacc gccgccgcag ccaaggtcga gcacgcgggc ggtggcatgg ccgcgcacat
     2161 gcgcctcgat ctggtcaaga tcggcgccgc tgctgtgcac cgcgctggtc acataatcct
     2221 gggcgcggac gctgtactgg gccgcggcga agctctgctg ggacgtgctc atcctggatc
     2281 tccccgacgg ccgatactgc cctgtccgct tcggatctga ccagccttgg cgtcgctgcg
     2341 gtccagccgc agcgggaacg cggtgcggct ggccggctgc accacgcggc cgacgatggc
     2401 cgccggcgat cccgccgcgt gcagcgccgc cagacaggcc tccgcctgct ccaccggcac
     2461 gccggccagc aggccgcccg aggtctgcgg atcgaccagc aaggcaaggt ccggctgcat
     2521 gcccagcggc ccgagcatgc ggcggttgtc cggcgccagg gtgctctcga tgccgcgcgc
     2581 cgccagcgcc gcggcgccgg gcagcgccgg caccgcatcc ggccacagca ccgccgccac
     2641 cttggaggcg cgcagcatct ccagcaggtg accggccagg ccgaacccgg tcacgtcggt
     2701 gcaggccgtg acgccatggt cgcgcaggac ctggaccgcc gcggcattgc tgacgcgcat
     2761 ggaatcgagc gccgccgcca gccaggccgc cttcgcctcg ccgcgcatgt ggccggccag
     2821 gatgaccccg gtgcccagcc gcttggtcag caccagcgca tcgcccaggc gcagcccgga
     2881 tttgcgcaac agccgcttcg gatcggcgag cccggtgacc gcgaagccca gcgcggcttc
     2941 cgcggcctcc gcactgtgtc cgccgaccag ggtgcagccg tcggctttca gcgtttccgc
     3001 cgcgcccagc agcatcgccg ccaggtcggc acgcatgcgg tcgccggaac tgtagggaac
     3061 gccggcgatc gccagcgcgg accagggccg ggcacccatc gcgtgcaggt ccgacaaagc
     3121 gtgtgccgcg gcgatctggc cgaacaggaa ggggtcgtcg ataaaggcgc ggaagtggtc
     3181 gacgctctgc acgatcgcct ggccggcggg ggggagcagc accgcggcat cgtcggcagc
     3241 gtccagcccg accagcacat ccgccccggg catctgcggc agggcggcca gggcgccggc
     3301 cagtacctcc gcccccacct tggccccgca accgccgcag cgcatcggat catcggaatc
     3361 catgcgccgc atcggcttct ggtacatccc gatccagcgg cgatcgaggt aatccttcca
     3421 ttgccagatc agccggccgg acaccgacag gccgttgcgc caggccaccg cccgcgcatc
     3481 gccaagcccc aggatcacca gcgcgt
//
LOCUS       DASSZH010000508         3005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1546179, whole genome shotgun sequence.
ACCESSION   DASSZH010000508 DASSZH010000000
VERSION     DASSZH010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546179"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1486)
                     /locus_tag="VFN42_11100"
     CDS             complement(<1..1486)
                     /locus_tag="VFN42_11100"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HET8997203.1"
                     /translation="MLGLMQPHGLMISSMLRHAARHHAKGEVVSRTHEGAIQRTNWSD
                     LERRARRLARVLQGLGVGAQDRVATLAWNNQRHLEVYYAAPGMQAICHTVNPRLHPDD
                     ITYIINHAGDAVLFADVSFAPLITAIASRIKDTVRAVIMMADVEGMPEVTLAPGMALH
                     CYEQLMAAADEDYAWPDFDENTASALCYTSGTTGRPKGVLYSHRSTVLHAYAIALPDV
                     LGLHATDRILPVVPMFHVNAWGIPHGTALTGAGLVLPGRHLDGFSMAQLLNQERVTVT
                     CGVPTVWLGLLQHLRASGERLETTRRIMTGGSAAPPLLIEAFRDEYGVAVEHGWGMTE
                     LSPVGTYNAPKPAQAGLDKDAAAQHMLKQGRVLPGVDMKIVDADGKELPWDGSAFGDL
                     LVRGPWVCSAYYGEEPGSALEPDGWFATGDVATIDPDGFMEITDRSKDVIKSGGEWIS
                     SIALENIALSHPDVAEAAVIAARHPKWDERPLLLVVPRPGHTIDP"
     gene            complement(1571..1768)
                     /locus_tag="VFN42_11105"
     CDS             complement(1571..1768)
                     /locus_tag="VFN42_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997204.1"
                     /translation="MSQSGPFSVEEHVHGDTVWVAIRARGAEWSWLTPEEAARLGREW
                     VARYGHSEAAPHAEHFLAAAE"
     gene            2049..2822
                     /locus_tag="VFN42_11110"
     CDS             2049..2822
                     /locus_tag="VFN42_11110"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET8997205.1"
                     /translation="MEWTEQVALVTGAARGIGRAIALRLAGQGAAVCVNFRADDEAAE
                     AVVQEIQAAGGRAVAVAADVTDDAAVESMIDRIENRFGGVSVLVNNAGVSLPANLDTY
                     DRERMARMRQVNVDGLIHATRAVIGGMRKRGYGRIVNISSIAGTGTALPDNAFYAATK
                     AEVNILTRRFAMELGPQGITVNAVAPGFIETEMTRRNRGGSDWDEVARRIGARAMMGR
                     VGRPEDIANAVAFLAAPDSGWITAQVLTVDGGRMDYIGH"
BASE COUNT          485 a          972 c         1049 g          499 t
ORIGIN      
        1 ccggatcaat ggtgtggccg gggcgcggca ccaccagcaa cagtggccgc tcatcccatt
       61 tcggatgccg cgcggcgatc accgcggctt cggccacgtc cggatgggat agcgcaatgt
      121 tctccagggc gatggagctg atccattcgc cccccgactt gataacgtcc ttcgaacggt
      181 cggtaatctc catgaaaccg tcggggtcga tcgtggccac atcgccggtg gcgaaccagc
      241 cgtcgggctc cagggcgcta ccgggttcct cgccataata ggcgctgcag acccagggac
      301 cgcgcaccag caggtcgccg aaggcggatc cgtcccaggg cagttccttc ccgtcggcat
      361 cgacgatctt catgtcgaca cccggcaata cccggccctg cttcagcatg tgctgggcgg
      421 cagcgtcctt gtccaacccg gcctgcgccg gtttcggcgc attgtaggtg ccgaccgggc
      481 tgagttcggt catgccccag ccgtgttcca ccgccacgcc atactcgtcg cggaaggcct
      541 cgatcagcag gggaggggcg gcggatccgc cggtcatgat ccgcctggtg gtttccagcc
      601 gttcgccact ggcgcgcaga tgctggagca gtcccagcca gaccgtgggc actccgcagg
      661 tgacggtgac ccgctcctgg ttcagcagtt gcgccatgct gaaaccgtcc aggtgccggc
      721 cgggcagcac cagcccggcg ccagtcagcg cggtgccgtg cggaatgccc caggcgttta
      781 catggaacat cggcaccacc ggcaggatgc ggtccgtcgc atgcagcccc agcacgtccg
      841 gcaacgcgat ggcataagcg tgcagcaccg tggagcggtg gctgtacagc acgcctttcg
      901 gccggccagt tgttccggat gtgtagcaga gtgcgctggc ggtgttctcg tcgaaatccg
      961 gccaggcgta atcctcgtcg gccgccgcca tcagttgctc gtagcagtgc agcgccatgc
     1021 caggggccag cgtcacctcc ggcatgccct ccacgtccgc catcatgatc accgcgcgga
     1081 ccgtatcctt gatgcgggag gcgatcgcgg tgatcagcgg ggcgaagctg acatcggcga
     1141 acagtaccgc gtcaccggcg tggttgatga tgtaggtgat gtcgtcggga tgcagccgcg
     1201 gattgacggt gtggcagatc gcctgcatgc cgggggcggc gtagtacacc tccagatggc
     1261 gctggttgtt ccacgcgagc gtggcgaccc gatcctgcgc gccgacgccc aggccctgca
     1321 gcacgcgcgc cagccggcgg gcgcggcgtt ccaggtcgct ccagttcgtc cgctggatag
     1381 cgccttcgtg tgtgcgggag acgacctcgc ccttggcatg atgacgcgcg gcgtggcgca
     1441 acatcgagga gatcatcaga ccgtgcggct gcatcaatcc gagcattgtc gttccctccg
     1501 tgccggccag gttcagccgt tccggcagaa tgatacgaca ggcgtcacgc aggctacagt
     1561 accggggctg ctattcagcg gcggccagga agtgctcggc gtgcggtgcc gcttcgctgt
     1621 ggccgtagcg ggccacccat tcgcggccga gccgggcggc ttcttccggg gtcagccacg
     1681 accactcggc gccgcgggcg cggatggcca cccagacggt gtcgccatgc acgtgctctt
     1741 ccactgaaaa cgggccggat tgtgacataa gagtatccaa tcggttcgag aggcggctgc
     1801 ccgccaatgg tcagccggat gcgtgccggc agtcggcacg ctggtgaaag ccgcagatcc
     1861 agacgggcat cttcacggat ccgggagcgc tgcgccgcag gatgtttgcc gggatgtccg
     1921 gagtcagtgc aatcgaaaaa tgcgggtggc gccgcagaaa tgccgcaaat cggccaacgg
     1981 gtgccgcgct gcgttgttgc tgccactggc ccaccgatcg gcgggccgcc agccaggaga
     2041 aagagtgtat ggagtggacc gaacaggtcg cgctggtaac cggcgcggca cgcgggatcg
     2101 gacgggcgat cgcgctccgc ctggcaggcc agggtgccgc ggtttgcgtg aatttccgcg
     2161 cagacgatga agcggcggaa gcggtggtgc aggagatcca ggccgcgggt ggccgcgccg
     2221 tcgcggtagc ggccgatgtg acggacgacg ccgcggtgga atcgatgatt gatcgtattg
     2281 agaaccggtt cggtggcgta tccgtcctgg tgaacaatgc cggcgtctcg ctgccggcca
     2341 acctggacac ttacgatcga gagcggatgg cccgcatgcg gcaggtcaat gtggacggcc
     2401 tgatccacgc gacgcgcgcg gtgatcggcg gcatgcgcaa gcgcggctat ggccggatcg
     2461 tcaatatctc ctccattgcc gggaccggca ccgcgctgcc ggacaacgcc ttctatgctg
     2521 cgaccaaggc tgaggtgaac atcctgaccc ggcgcttcgc catggaactg gggccgcagg
     2581 ggatcacggt gaacgcggtt gctcccggct tcatcgaaac ggaaatgacc cggcgcaatc
     2641 ggggcggatc cgactgggac gaagtcgccc ggcggatcgg cgcgcgcgcc atgatggggc
     2701 gggtcggccg gccggaggac atcgcgaatg cggtggcatt tctcgccgcc ccggactcgg
     2761 gttggatcac cgcgcaggtg ctgacggtgg atggcgggcg gatggactac atcggccact
     2821 gatgccgctg cgcccacgct tcggccgcaa ccggcgttga cctgcgcggt cctggcttcg
     2881 gctgcgcatc gagccaagca ccggcggcgg cgatccgttc gacccgaccc gccgcccatc
     2941 gcactggcga tggacggcgg gttcgcgctg ctgcgcggat ctgcggccga tgtggcgttc
     3001 agtcc
//
LOCUS       DASSZH010000509         2436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_693658, whole genome shotgun sequence.
ACCESSION   DASSZH010000509 DASSZH010000000
VERSION     DASSZH010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693658"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VFN42_11115"
     CDS             complement(<1..807)
                     /locus_tag="VFN42_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET8997206.1"
                     /translation="MPVETVIQAARDLLGDRLATSTALREQHAHGEDTHPPSMPDAVA
                     FVETTDEVARILALCNQEHVPVVPWGAGTSLEGHVTPARGGITLDMSRMTRIIDVSQP
                     DMDCRVEAGVTRAQLNTHLRDAGLFFPVDPGTAACTIGGMCGTRASGTNAVRYGTMRE
                     NVLGLTVVLADGRVIKTGGRVRKAANGYDLTRLFLGSEGTLGVVTEIQLRLHGIPEAI
                     SSATCQFPDLGSAVDTVIAILQMGIPVARMELLDDVQVEACIRYSKLEGLA"
     gene            complement(905..1510)
                     /locus_tag="VFN42_11120"
     CDS             complement(905..1510)
                     /locus_tag="VFN42_11120"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET8997207.1"
                     /translation="MRYLTDFADQAVIMPLVLAVSVALWWQGWKRGALIWLVVTGGTF
                     AAILTAKLVFLGCAPVFGPLNLYSPSGHVAASSVVAGGLAAILTGRLRSILPAALVTA
                     IVISISRILLGAHTVPEVIVGGMIGMAGAAALMHFTGTPPRLRVKPLVAVVLIVATAF
                     HGLRLPAEAAIRRTAFSAAQIIPACRGAAEAYRHRDLHEEF"
     gene            1646..>2436
                     /locus_tag="VFN42_11125"
     CDS             1646..>2436
                     /locus_tag="VFN42_11125"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET8997208.1"
                     /translation="MTVAESVIDSLIAHGIDILFALPGVHNDHLFDAAHKAQHRIRLI
                     HPRHEQTAAYMALGAALVTGRPQAFAVVPGPGVLNASAALLTAYGLGAPVLALAGQIP
                     SFAIDRGHGHLHEIHDQRGLLRHITKATERITSPADASELVARSIALSLSGRTGPVAL
                     ECAMDVWGQAGDFAPVAPIPVTRPEADAVAVSRAADILAGASRPLIVVGGGALDAGAE
                     VQAVAELLHAPVMSFRRGRGVIPTSHPLAVSMTEGHGLWKTADAV"
BASE COUNT          391 a          795 c          845 g          405 t
ORIGIN      
        1 tgcgaggccc tcaagcttcg aatagcggat gcaggcttcc acctgcacgt cgtccagcag
       61 ttccatccgc gcgaccggga tgcccatctg caggatggcg atcaccgtat cgaccgcgct
      121 gccgaggtcg gggaactggc aggtggctga ggagatcgct tccgggatgc catgcagccg
      181 cagctggatt tccgtcacca cgcccagcgt gccctcagag ccgaggaaca gccgcgtcag
      241 gtcgtagccg ttggccgcct tgcgtacccg gccgccggtc ttgatgaccc gcccatcggc
      301 cagcacgacg gtcaggccca gcacattctc ccgcatcgtg ccgtagcgga cggcattggt
      361 tcccgacgcg cgggtgccgc acatgccgcc gatcgtgcag gcggccgtgc cgggatcgac
      421 ggggaagaaa agccccgcat cacgcaaatg ggtattcagc tgcgcccgtg tcacgccggc
      481 ttcgacccgg cagtccatgt ccggctggct gacatcgatg atgcgcgtca ttcgtgacat
      541 atccagtgtg atgccgccgc gcgccggcgt gacatgcccc tccagcgatg tgccggcacc
      601 ccagggcacg accggcacat gctcctggtt gcacaacgcg aggatgcggg cgacttcgtc
      661 ggtggtttcc acgaacgcca cggcatcggg catcgacggc ggatgggtgt cctcaccatg
      721 cgcatgctgc tcccgcagtg ctgtgctggt ggccaggcga tcacccagaa ggtcccgggc
      781 ggcttggatg acggtttcga cgggcatgac gattcctgtg cgagcctttc aggtagcaat
      841 gcgatcctgc gggccgcgtg tccagagtgg cgttggtgcg gtgggttggc gcggtggccg
      901 ggcttcagaa ctcttcgtgc aggtcgcggt gccggtaggc ctccgccgca ccgcggcagg
      961 ccgggatgat ctgcgcggcg ctgaacgcgg tgcggcggat ggcggcttcg gccggcaggc
     1021 gcaggccgtg gaacgccgtg gcgacaatca gcaccacggc gaccagtggc ttcacccgca
     1081 gccgcggcgg cgtgccggtg aagtgcatca acgccgccgc acccgccatg ccgatcatgc
     1141 cgccgacgat cacctccggc accgtgtggg cgccgaggag gatgcgggag atactgatga
     1201 cgatcgctgt caccagcgcc gcgggcagga tgctgcgcaa acgtccggtc aggatcgctg
     1261 ccaatccgcc cgccacgacg gacgatgcgg cgacatgtcc gctggggctg tagagattga
     1321 gcggcccgaa caccggggcg cagcccagga acaccagctt ggcggtcagg attgcagcga
     1381 atgtgccgcc ggtgacaaca agccagatca gtgcgccgcg cttccagcct tgccaccaca
     1441 gggcgacgga tacggcgaga accagcggca tgatcaccgc ctggtcggcg aagtcggtaa
     1501 gatatctcat tgatgccggg tcttagctga aaaccgttaa ggagtggttc agaatcgcgg
     1561 ggcggccgtc ccccgccggt tcccagcggc gcatttatac aggacgatgc ggccagcgga
     1621 atgtcaggag atcgttatgc cccgcatgac tgttgccgaa tcagtcattg attcgttgat
     1681 tgcacatggg attgacatcc tgttcgcgct gcccggcgtg cacaatgacc atctgttcga
     1741 cgccgcgcat aaggcgcagc accgcatccg cctgatccat ccccggcatg agcagacggc
     1801 tgcctacatg gcgctaggcg cggcgctggt gaccgggcgg ccgcaggcat tcgccgtggt
     1861 acccgggccg ggcgtgctga atgcctcggc ggcgctgcta acggcttatg gtctgggggc
     1921 gccggtcctg gccctggccg gccagatccc atccttcgcc atcgaccgcg gccacggcca
     1981 cctgcatgag atccacgacc agcgcggcct gctgcggcac atcaccaagg cgaccgagcg
     2041 gatcacatcg ccggcggacg cgtcggagct ggtcgcgcgg tcgatcgccc tgtcgctgtc
     2101 cggtcgcacc ggaccggtgg cgctggaatg cgcgatggac gtctggggtc aggcggggga
     2161 tttcgcgccg gtggcgccga tcccggtaac ccggccggaa gccgacgccg ttgccgtcag
     2221 ccgtgcagcg gatatcctgg ccggcgcatc gcgtccgctg atcgtggtgg ggggcggcgc
     2281 gctggacgcc ggtgcggaag tacaggccgt cgcggagctg ctgcacgcgc cggtgatgtc
     2341 atttcgtcgt ggccgcgggg tgatcccaac gtctcatcct ctggcggtgt cgatgaccga
     2401 agggcacggt ctttggaaga ccgccgatgc ggtgtt
//
LOCUS       DASSZH010000510         7093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_881323, whole genome shotgun sequence.
ACCESSION   DASSZH010000510 DASSZH010000000
VERSION     DASSZH010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7093
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881323"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /gene="ugpC"
                     /locus_tag="VFN42_11130"
     CDS             complement(<1..741)
                     /gene="ugpC"
                     /locus_tag="VFN42_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HET8997209.1"
                     /translation="MANVSIRNVRKRFGPVEVLHGVNVEIQDGEFVILVGPSGCGKST
                     LLRMIAGLENISSGEIAIGERVVNNVAPKERDIAMVFQNYALYPHMTVRDNMAFALSL
                     AKAPKSVIDEKVQRAAQILGLTQYLNRYPRQLSGGQRQRVAMGRAIVRDPQVFLFDEP
                     LSNLDAKLRVQMRAEIKELHQRLTTTTVYVTHDQIEAMTMADKIVVMNSGNVEQIGAP
                     LELYDRPANLFVAGFIGSPAMNFLKGKVV"
     gene            complement(893..2515)
                     /locus_tag="VFN42_11135"
     CDS             complement(893..2515)
                     /locus_tag="VFN42_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HET8997210.1"
                     /translation="MKVGEAVAEIMKREGIEILCGYPVNHLLEFAAKADIRPVIVRQE
                     RIGLHMADAISRVTSGRKIGAFCMQHGPGAENAYGGVAQAYSESIPLLVIPQGYPRRI
                     ANIDPNFNSAREMRGIAKRAEPVNIPAETANVLRRAFSAIRNGRSGPAVVEIPTDIWN
                     EEIGELDYTPVGVHKYAPDPVDVRKAAAAILAAKRPVIYAGTGVHWAEAWAELKEFAE
                     LLGAPVTTSLGGKSSFPENHPLSLGSGGNAVPKPVHHFVQNADVIIGIGCSFTETNFG
                     IKFPKKTFIHATLDPNHLNKDVVSAVGLVGDAKLTLAALIGELRQSIKSNRDPSAVVA
                     EITKVREEWMEQWRPLLNSNDAPLSPYRVIWELMNTVDGKNTIITHDAGSPRDQISPF
                     WVSTEPLSYIGWGKTTQLGMGLGLAMGAKLAKPDKLCINLWGDAAIGFTGMDFETAVR
                     ERIPILSILLNNFSMAIELKVMPVSTEKYRSTDISGNYADMAKAFGGYGERVTQPGDI
                     KAAIQRGIAQTQAGKPALLEFITCKETRVSKF"
     gene            2734..3444
                     /locus_tag="VFN42_11140"
     CDS             2734..3444
                     /locus_tag="VFN42_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET8997211.1"
                     /translation="MRASDVMTPTVVTVDPDMTVQDLAKLLSESGVSGAPVMDSAGKL
                     VGIVSEGDLLHRAELGTAHHTERRRSWWLRHFAAAEARDYAKSHGRLVKDVMTTNVAT
                     VEEETSLAEVATLLERRRIKRVPVLRDGRLVGIITRSNLVRALAAAKPSTPLMTDHDD
                     DDRAIRLRLLADLNGQNWATRVWPQDILVNHGVVTLWFSSDEPEANRRAIRVVAENVP
                     GVHDVQENIVPAPLLPTF"
     gene            3710..3958
                     /locus_tag="VFN42_11145"
     CDS             3710..3958
                     /locus_tag="VFN42_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997212.1"
                     /translation="MSGIPLAMRAVQVTNAVCAFAFRNDSGRTLQMARIHYYHGGRTD
                     NDILPGLRPGQVLGGWAAFSVEDRCSNVSVQVYDASWR"
     gene            complement(4003..6270)
                     /locus_tag="VFN42_11150"
     CDS             complement(4003..6270)
                     /locus_tag="VFN42_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET8997213.1"
                     /translation="MRPRPALMLGWMVMVVVVASDGLTLLSASSFISHSVPLRAQDVL
                     NREQQLVRVAIDHGDPARSLARRIAALGHGFAGRLAFLPVLADGSIDTASAAGFVTPD
                     VANQLDFLLRPTFRQVQFAGESWFVLSTPINWVGAASGLSRGVLVLATQRAPLALEAQ
                     TEREGLIGTALLTLVLLGYALVGVSQWLGTQLEGREIRLAAAKGELELALAHMSDGLC
                     VFDRHNRVTVFNQQFCDVVGLPPGSVRRGASFTDLIALSLAIGNHSGRDAEEVMAEHH
                     ALVEARRSIKLHQATHDGRTIETEYSPTAQGGWLLACADITERLRVHGEIAYMAEHDG
                     LTGLLNRAAFAGHLERLVTMATAEQPLTLMYLDLDHFKDINDCWGHPAGDALLCTVAD
                     RLRHSLREGQDAAYRLGGDEFAVLLTGIDAQEAEGLARRMLRTINQPVEIAGALHAPR
                     TSIGIAAAPRDAASADRLLKCADVALYAAKGSGRNALRTFSAELEHASNGHSATERML
                     HAALAEQQFELWYQPVVALPSRKLVGFEALLRWNHPKRGLLLPEVFIGMAEKSQAIED
                     IGAWVIAQACADASGWPEEMVLAVNVSAEQLHRRELAGQVQRALRRSGLAAGRLELEV
                     TETAIIRDPPFAFSVLREVQALGARVALDDFGTGYSSLSFLTQFRFDLIKIDRSFVTD
                     MVDRPDCAAIVDALSSLGSHLGLVTTAEGIETAEQLRRVGELGCRRAQGFLLGMPMRA
                     EQARELAGSRAVAAA"
     gene            6516..6710
                     /locus_tag="VFN42_11155"
     CDS             6516..6710
                     /locus_tag="VFN42_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HET8997214.1"
                     /translation="MWLLVLLLLPFIGLLWVPFYNISEPSFLGFPFFYWYQLAWVPVS
                     SLLIWLAYKGLPSDDTDQPE"
     gene            6688..>7093
                     /locus_tag="VFN42_11160"
     CDS             6688..>7093
                     /locus_tag="VFN42_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HET8997215.1"
                     /translation="MTPISPSEIAVCLSSLALVVAVGISARWWRRGGRSMGELGEWGL
                     AGRQFGTWITWFLLGGDFYTAYTIIAVPALVYSTGAAGFFALPYTIVVYPVVFAAMPA
                     LWRFAHRHGCITAADVVQVRYGSRGLALAVAVT"
BASE COUNT         1111 a         2327 c         2404 g         1251 t
ORIGIN      
        1 gacaaccttg cccttcagga agttcatcgc cggcgagccg atgaatcccg cgacgaacag
       61 gttagccggg cgatcgtata gctccagcgg cgcgccgatc tgctcgacat tgccgctgtt
      121 catcaccacg atcttgtcgg ccatcgtcat cgcctcaatc tggtcgtgcg tgacgtacac
      181 cgtggtggtg gtcaggcgct ggtgcagctc cttgatctcc gcgcgcatct gcacgcgcag
      241 cttggcgtcc aggttggaca gcggctcgtc gaacaggaac acctgcgggt cgcgcacgat
      301 ggcgcggccc atcgccacgc gctgccgttg cccgccggag agctggcgcg gatagcggtt
      361 gaggtactgg gtcaggccca gaatctgcgc ggcacgctgc accttctcgt cgatcaccga
      421 cttcggcgcc ttggccagcg acagcgcgaa ggccatgttg tcgcgcaccg tcatgtgcgg
      481 gtacagcgcg tagttctgga acaccatcgc aatgtcgcgt tctttcggcg cgacattgtt
      541 caccacgcgt tcgccgatgg cgatctcgcc ggacgagatg ttctccagcc cggcgatcat
      601 gcgcaacagc gtcgatttgc cgcagccgga ggggccgacc aggatgacga actcgccgtc
      661 ctggatttcg acattgacgc cgtgcagcac ctcgaccggg ccgaagcgct tgcgcacgtt
      721 gcggatggaa acattggcca tactgtatct tccgtaggct gcgaaacttc cgggtgggga
      781 acgtgaaaaa gggccgtgcc gatcgttctt gcgaactgtc cgcacgaccc ctcccctggc
      841 cccctccact tgggggaggg gggaacactt ggcggtatcc tacctcaacc ggtcagaatt
      901 tggaaacccg ggtctccttg caggtgatga actccagcag cgccggcttg ccggcctggg
      961 tctgcgcgat gccgcgctgg atcgccgcct tgatgtcgcc gggctgcgtc acgcgctcgc
     1021 cgtagccgcc gaacgccttg gccatgtcgg cgtagttgcc ggaaatatcc gtggaccggt
     1081 acttctcggt ggagaccggc atcaccttca gctcgatcgc catggagaag ttgttcagca
     1141 ggatcgacag gatcggaatg cgctcgcgca ccgcggtctc gaagtccatg ccggtgaagc
     1201 cgatcgccgc gtcgccccac aggttgatgc acagcttgtc cggcttggcc agcttggcgc
     1261 ccatcgccag gcccagcccc atgccaagct gcgtggtctt gccccagccg atataggaca
     1321 gcggttccgt ggagacccag aacggcgaga tctggtcgcg cgggctgccg gcgtcatggg
     1381 tgatgatggt gttcttgcca tcgaccgtgt tcatcagctc ccagatcacg cgataggggc
     1441 tgagcggcgc atcgttggag ttcagcagcg gacgccactg ctccatccac tcctcgcgca
     1501 ccttggtgat ctcggccacc acggcggaag gatcgcggtt ggacttgatg ctctggcgca
     1561 gctcgccgat cagcgcggca agcgtcagct tcgcatcgcc gaccaatccc accgcgctga
     1621 ccacgtcctt gttcaggtgg ttcggatcca gcgtggcgtg gatgaaggtc ttcttcggga
     1681 acttgatgcc gaaattggtt tcggtgaacg agcagccgat gccgatgatc acgtcggcgt
     1741 tctgcacgaa atgatgcacc ggcttcggca ccgcgttgcc gccggagcca agcgagagcg
     1801 gatggttctc cggaaacgaa ctcttgccgc cgaggctggt ggtgaccggc gcgccaagca
     1861 gttcggcgaa ttccttgagc tcggcccagg cttcggccca gtgcacgccg gtgccggcat
     1921 agatcaccgg gcgcttcgcc gccaggatcg ccgcggcggc cttgcgcacg tcaaccggat
     1981 cgggcgcgta tttgtggacg ccgaccggcg tgtagtcgag ttcgccgatc tcctcgttcc
     2041 agatatccgt ggggatttcc accaccgccg gtccgctgcg gccgttgcgg atcgcgctga
     2101 acgcgcggcg cagcacgttg gcggtttccg ccgggatgtt caccggctcg gcgcgcttgg
     2161 cgatgccgcg catctcgcgc gccgagttga agttcgggtc gatgttggcg atccggcgtg
     2221 gatagccctg cgggatcacc agcagcggaa tggattcgga ataggcctgc gcgacaccgc
     2281 catacgcatt ctcggcaccc ggcccgtgct gcatgcagaa ggcaccgatc ttcctgccgg
     2341 aggtcacgcg gctgatcgcg tcggccatgt gcaggccgat ccgctcctgc cgcacgatca
     2401 ccgggcggat gtcggccttg gcggcgaatt ccagcaggtg gttcacggga tagccgcaga
     2461 ggatctcgat gccctcgcgc ttcatgattt ccgcaacagc ttcgccgact ttcatgtggc
     2521 gcgttccctc tgctttgggt ggctcttcgg ctccggtcct ggcggccggt agcgcacccg
     2581 ttcagggtag ggccggtgcc gagcggtggc aaccagggcg gcggtcgcgg gggggcgcca
     2641 cggccggctt tgtttcgccg gacaaagcgg gtaaattgcg gagcggtcgt cgctgccgcg
     2701 atggccgccc ggcacaatgc gggagggtag cgcatgcgtg catccgacgt gatgacaccg
     2761 accgtggtca ccgtggatcc cgacatgacg gtgcaggacc tggcaaagct gctgtccgaa
     2821 tccggcgtca gcggcgcgcc ggtgatggat tccgcgggca agctcgtggg catcgtcagc
     2881 gaaggcgacc tgctgcatcg ggccgagctg ggcactgcgc atcacacgga gcggcggcgt
     2941 tcctggtggc tgcggcattt cgcggcagcc gaggcgcggg attacgcgaa gtcccatggc
     3001 cgcctggtga aggatgtcat gacgaccaac gtggcgacgg tcgaggagga gacgtcgctg
     3061 gccgaggtcg ccaccttgct ggagcggcgg cggatcaagc gcgtcccggt gctgcgcgac
     3121 ggccggctgg tcggcatcat cacccgcagc aacctggtgc gcgcgctggc ggcggcgaaa
     3181 ccgtcgacgc cgctgatgac ggaccatgac gacgacgacc gcgcgatccg cttgcggcta
     3241 ctggccgatc tgaacggtca gaactgggcg acgcgggtgt ggccccagga catcctggtc
     3301 aaccatggtg tggtcacctt gtggttttcc tccgacgagc ccgaggcgaa ccgccgcgcc
     3361 atccgcgttg tcgcggaaaa cgtgccgggc gtgcacgacg tgcaggagaa catcgtcccg
     3421 gcgcccctgc tgccgacgtt ctgagacgct gttctgcccg gcggcaatcg cgcggcctaa
     3481 ccagaatggg tgatgcggcg gcgttcgcgg cggcgcaaag ctgtgcgcgt tccggctggg
     3541 tgccgggatg tccggacccg caccagggcc ggctgggaga cgttcagaag ggaacaggac
     3601 catgcgactc acccatgcaa agcgctcatg gacgggtgcg tttgcgcttt cgctggcagt
     3661 ctgttgcgcc ggatcggccg gtgcgaatga atggggaccg tggcgcgaaa tgtcgggcat
     3721 cccgctcgcg atgcgtgccg tgcaggtcac caacgcggtc tgcgcctttg ctttccgcaa
     3781 tgactccggg cgtacgctgc agatggcgcg gatccattac taccacggcg gccgcacgga
     3841 caacgacatc ctgccgggcc tgcgtcccgg ccaggtgctg ggcggctggg cggcgttcag
     3901 cgtcgaggac cgctgttcca acgtctcggt gcaggtctac gacgcgtcct ggcgctagct
     3961 agcgctgcat tcgggaaggg cgacgcggcc gtggcttgcc tgctacgccg cggcgaccgc
     4021 gcgcgagccc gccagctcgc gcgcctgctc cgcccgcatc ggcatgccca gcaggaaccc
     4081 ttgcgcccgg cgacagccaa gttcgccgac ccggcggagc tgttcggcgg tctcgattcc
     4141 ctcggccgtc gtcaccagac ccagatgtga gccgagcgaa ctcaacgcat cgacgatcgc
     4201 cgcacaatcc ggccggtcca ccatgtcggt gacgaaggaa cggtcgatct tgatcaagtc
     4261 gaagcggaac tgtgtcagga aactcagcga cgagtagccg gtgccgaaat cgtccagcgc
     4321 cacccgcgca cccagtgcct gcacctcccg cagcaccgag aacgcgaagg gaggatcccg
     4381 gatgatcgcg gtctccgtca cctcgagttc cagccgtccg gccgcaaggc cgctgcggcg
     4441 cagggcgcgc tgcacctggc cggcaagttc ccggcgatgc aactgctccg ccgaaacatt
     4501 caccgcgagc accatctcct cgggccagcc ggaggcgtcg gcgcaggcct gggcgatcac
     4561 ccaggcgccg atgtcctcga tcgcctggga cttctccgcc atgccgatga acacctccgg
     4621 caacagcaac ccgcgtttcg gatggttcca gcgcagcagc gcctcaaatc caaccagctt
     4681 gcgcgacggc aatgcgacaa ccggctggta ccacagctcg aactgctgct cggccagtgc
     4741 tgcatgcagc atgcgctcgg ttgccgaatg cccgtttgac gcatgctcca gctcggccga
     4801 gaaggtccgc agcgcgttgc gccccgatcc tttcgccgca tacagcgcga catcggcgca
     4861 tttcagcagg cggtccgcgc tggccgcatc gcgcggcgcg gcggcgatgc cgatactggt
     4921 gcgcggcgcg tgcaaggcac cggcgatctc caccggctgg ttgatcgtcc gcagcatgcg
     4981 ccgcgccaag ccttcggctt cctgcgcgtc gatgccggtc agcagcacgg cgaactcgtc
     5041 gccgccgagg cgataggcgg catcctggcc ttcccgcagc gaatggcgga ggcggtccgc
     5101 cacggtgcac agcaacgcgt cgccggccgg atggccccag caatcgttaa tgtccttgaa
     5161 atggtccagg tcgagataca tcagcgtcag cggttgttcc gccgtcgcca tcgtcaccag
     5221 ccgttcgaga tggccggcga aggcggcgcg gttgagcaat ccggtcagtc cgtcatgctc
     5281 cgccatgtag gcgatctcgc cgtggacccg caggcgttcg gtgatatcgg cacaggccag
     5341 cagccagccg ccttgcgcgg tcggcgagta ctccgtctcg atcgtgcggc cgtcgtgcgt
     5401 cgcctggtgc agtttgatgc tgcgccgcgc ctccaccaag gcgtggtgtt cggccatcac
     5461 ttcctcggcg tcgcggccgg agtgattgcc gatcgccagg ctcagcgcga tcaggtcggt
     5521 gaagctggcg ccgcgccgga ccgagcccgg cggcaggccg acgacgtcgc agaattgctg
     5581 gttgaagacg gtgacccggt tgtgccggtc gaacacgcac agcccgtcgg acatatgcgc
     5641 cagggccagt tccagctcgc ccttcgcggc ggcgaggcgg atttcccgtc cctccaactg
     5701 cgtgccgagc cactggctga cgccgaccag ggcgtagccg agcaggacga gagtgagcag
     5761 cgcggtgccg atcagccctt cccgttcggt ttgcgcctcc agggccagcg gcgcgcgttg
     5821 cgtggcgagc accagtacgc cgcggctcag gccggaggcg gcgccgaccc agttgatcgg
     5881 ggtggacagc acgaaccagc tctcgccggc gaactgcacc tggcggaagg tcggccgcag
     5941 caggaagtcg agctggttgg ccacgtccgg cgtgacgaat ccggccgcgg atgcggtatc
     6001 gatgctgccg tccgccagca ccggaaggaa ggcgagacgg ccggcaaatc catgcccgag
     6061 cgcggcgatc cgccgcgcca aggaccgtgc cggatcgccg tggtcgatcg ccacccgcac
     6121 caattgctgc tcgcggttca gtacgtcctg cgcgcgcagg gggacggagt ggctgatgaa
     6181 gctggatgcg ctgagcagag tgagcccgtc ggaggcgacg accacgacca tgaccatcca
     6241 gcccagcatc aaggcgggac gcgggcgcat cagcccggtc cgcaggcggc gggcgatgcg
     6301 gccgacggcc tctcgaaggg cggggatcgg cgggcgtttc ctcatcgagc cttgggttgc
     6361 tgtggccggc tggcgggttt tgcggtcacc cgggttgcaa aactgctacc ctttgtcgca
     6421 gcaaagcgaa acctttattc aaggccgggc aaggcaaact gccgctccgc catccgggct
     6481 ggcagtccgg cggcaattct ttgcggagga gcccgatgtg gctgcttgtt cttctgctgc
     6541 tgccgttcat cggcttactg tgggtgccgt tttacaatat aagcgagccc agtttcctgg
     6601 gatttccgtt cttctactgg tatcagttgg cctgggttcc ggtgagttcg ttgctgatct
     6661 ggctggctta caaaggtcta ccgagcgatg acaccgatca gcccgagtga aatcgccgtc
     6721 tgcctgtcca gcctggcgct ggtggtcgcg gtcggcatct cggcccgctg gtggcggcgc
     6781 ggcgggcgct ccatggggga gctgggtgag tggggcctcg ccggacggca attcggcacc
     6841 tggatcacct ggttcctgct gggcggcgat ttctacaccg cctacacgat catcgccgtg
     6901 ccggcgctgg tgtacagcac cggggccgcc ggtttcttcg cgctgcccta cacgatcgtg
     6961 gtgtatcctg tggtgttcgc ggccatgccg gcgctgtggc gattcgccca ccggcacggc
     7021 tgcatcaccg ccgccgacgt ggtgcaggtg cggtacgggt cgcgcggctt ggcgctggcc
     7081 gtggcggtga ccg
//
LOCUS       DASSZH010000511         3769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1000921, whole genome shotgun sequence.
ACCESSION   DASSZH010000511 DASSZH010000000
VERSION     DASSZH010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000921"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            345..641
                     /locus_tag="VFN42_11165"
     CDS             345..641
                     /locus_tag="VFN42_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET8997216.1"
                     /translation="MHVFLDDAALDALCRRYAIRRLSLFSSTLKGVDRPDSDVDLLVE
                     FQPGAKPSLLTMAAIELELSPLLGGRKVDLRTAGDLSRYFRDEVVRTAEPQYGS"
     gene            complement(852..1229)
                     /locus_tag="VFN42_11170"
     CDS             complement(852..1229)
                     /locus_tag="VFN42_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010668249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997217.1"
                     /translation="MRNARTAILAIPVAAMLALPAAAPAQAHDRDGGAAIAAGIIGLG
                     IGAVLAAGAASAPVYAAPVYAAPAYPAPVYAPPPPAYGYTPVYAPPPPPVYYAPRPVY
                     APPPAYVERPIHAGWRYDDDDDD"
     gene            complement(1574..2482)
                     /locus_tag="VFN42_11175"
     CDS             complement(1574..2482)
                     /locus_tag="VFN42_11175"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8997218.1"
                     /translation="MKGTVGIVGLGIMGGAIARNLAAAGWHVLGFDIDAGRCAAVADA
                     GVQACADAAAVARGAPVVLFSLPSPAASLTSATAIAAAGLERRVVIEASTLTLNDKLA
                     VEQVLRTAGHVPLDCPISGTGSQAINKDLVIYASGDAQAIAAQALLFADYARAVHDLG
                     AYGNGSRMKFVANLLVAIHNVASAEAMALGVKAGLDPQQVVALVQAGAGTSRVFELRA
                     PMMAERQYQPATMKVDIWQKDMAIIGQFATGLGCPTPLFSASLPVYAAAMANGHAADD
                     TAAVYAVLEAMAGITGGHAGTHTGPE"
     gene            complement(2500..3495)
                     /locus_tag="VFN42_11180"
     CDS             complement(2500..3495)
                     /locus_tag="VFN42_11180"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8997219.1"
                     /translation="MRIVALEEHFVAPDVVARIDPALSQRRGFPAPGVVWGQAIKRGE
                     LADLGPTRIADMDAAGISVQVLSVSGPGADLVEGTAGVQLARDYNDALARAVAAHPGR
                     LAGFAHLPMLSPDAAADELERCVRDLHFHGALINGLTAGKCLDHPSFDPILARAAALE
                     VPIYIHPGIAEEPVRRVYYDGLPGMMSFAMATSAWGWHIETAVQALRLVLSGALDRHR
                     KLNIIIGHMGEAIPFMLARCEQVLAAEAAKVLQRTVTQTILDQMWITTSGFFTVPPFL
                     NALETFGADRILFSVDYPFAANKTGRAFLDTLPVSPADRTKIAHGNADKLLRLPA"
     gene            complement(3599..>3769)
                     /locus_tag="VFN42_11185"
     CDS             complement(3599..>3769)
                     /locus_tag="VFN42_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006116305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8997220.1"
                     /translation="HDMASARRIGDSAAMLHRGKIVWSGPASELLESGNPMVDQFTHG
                     RREGPIEMELRR"
BASE COUNT          653 a         1296 c         1214 g          606 t
ORIGIN      
        1 gcacgccatt cttaaggttt tgttaatcga agcctatgca ttgggcagtc cccaatccgc
       61 gtgcgcgatc gttgatccgt acctaatctt tccgggcgta ctcagcccct gatctctggc
      121 cgggacctgc acgagcagcc cttcgcccga agcggcggcc cgcacggctt cgcgcatgca
      181 gcgcctatcg cggcttgccg ccgacgcgtt tgccgcgcgg tcggtcgtcg ctaggcgaga
      241 ccagggccgg aggcttgcct gcggcaatgc gggatattac cgagaacagg cgccgaccgc
      301 ggccacatga cgggccgtga gccctgcgtc tgcaaagtga tgccatgcac gttttcttgg
      361 acgacgccgc cctggacgcc ctctgccgac gctacgccat ccgccggctg tcgctgttca
      421 gctccacact gaagggcgtc gatcggccgg acagcgacgt ggacctattg gtggagttcc
      481 agccgggcgc caagccatcc ctgctcacca tggctgcaat cgaacttgag ctttcgcctc
      541 tgcttggggg acgcaaagtt gatctgcgca cagccgggga cctgagccgg tatttccgcg
      601 acgaagtcgt gcgtacggcg gaaccgcaat atggaagctg agggcggcaa ccggctgacg
      661 tccatggccg cagtcctcgg tcacgcaatc catttcactg aagggcgcag ccgcgcggac
      721 ctggacagcg acccgagcac aacagtccga ggaccatcgc cgccccgggg ccgctccgca
      781 gttacgcgtg cacggccggc ggtatcctcg ggtcggactg cacgatgggc gcgcaagggc
      841 ctggcgccgc atcaatcatc gtcatcatca tcatagcgcc atccggcgtg gatcggccgc
      901 tccacgtacg ccggcggcgg ggcatagacc ggccgcgggg cgtagtagac gggcggcgga
      961 ggcggcgcat agacgggcgt atagccgtat gccggcggag gcggtgcata gacgggcgcc
     1021 gggtaggccg gggcagcata gacaggggca gcatagacgg gggccgaagc cgcaccggcg
     1081 gcaaggacag cgccgatgcc cagcccgatg atgccggcgg caatagcggc gccaccatcc
     1141 cggtcgtgcg cttgcgccgg tgcagcggcg ggaagcgcca gcatggcggc gacggggatg
     1201 gcaagaatgg cagtgcgagc gttacgcatt gggcaatccc tcttgtggga acgctttgac
     1261 gatcattccc gatcggggct gcattgtgtc agccaaccgg cagaacggtg cgttgctgca
     1321 atttaacgcc tccgctacca gcgcgcgagc catcgaccgg cgccttcgtg cggtttcccg
     1381 catctgcact gcggaatgca tgaaggcgcg agattggtga tctctgcctt cgctgcggcg
     1441 atcgtgttcg ccgcggcgcg caagacggcc ggccttcgcc ggccatgctg gcggactcgc
     1501 cacgctcggc ctcatgcggc aattggatga cagcaccctt cggcgcagcg acgtggtaca
     1561 gcttgcgtca gcgtcactcc ggcccggtat gcgtccccgc atggccgcct gtgattcccg
     1621 ccatcgcctc cagcacggca tacaccgccg cggtgtcgtc cgcggcatgg ccgttcgcca
     1681 tcgctgccgc ataaaccggc aggctggccg agaacaacgg tgtcggacag ccaagcccgg
     1741 tagcgaactg gccgatgatc gccatgtcct tctgccagat atccaccttc atcgtggccg
     1801 gttgatactg ccgctccgcc atcatcggcg cgcgcagttc gaacacgcgc gacgtgccgg
     1861 cgccggcctg caccaatgcc accacttgct ggggatccag cccggccttc acacccagag
     1921 ccatcgcctc ggcgctggcg acattgtgga tcgccaccag cagattggcg acgaacttca
     1981 tccggctgcc attgccatac gcgcccaggt cgtgcacggc ccgcgcataa tccgcgaaca
     2041 gtagcgcctg cgcggcgatc gcctgtgcat cgccgctggc atagatcacc aggtccttgt
     2101 taatcgcctg ggacccggtg ccactgatcg ggcaatcgag cggaacatgc cccgctgtgc
     2161 gcagcacctg ttccactgcc aacttgtcgt tcagcgtcag cgtggaagct tcgatcacga
     2221 cacggcgctc gagcccggct gccgcaatcg ccgtggcgct cgtcagcgaa gctgcgggac
     2281 tcggcaggct gaacagcacg accggcgcgc cacgtgccac agcggcagca tcggcgcagg
     2341 cctgcacgcc ggcatccgcc actgcggcac agcgtcccgc gtcgatgtcg aatcccaaaa
     2401 catgccagcc agcagctgcc aggttacgcg cgatggcgcc acccatgatg cccagaccga
     2461 cgattccaac ggtacccttc atgtccccgc ctccctgtct caggccggca gccgcaacag
     2521 tttgtcggcg ttgccatgcg cgatctttgt gcggtcggca ggagacaccg gcagcgtatc
     2581 caggaacgcc cgcccggtct tattcgcggc gaacgggtag tccaccgaga acagaatccg
     2641 gtcggcgcca aacgtctcca acgcgttgag gaatggcggc acggtgaaga acccgctggt
     2701 ggtgatccac atctggtcca ggatggtctg ggtcaccgtc cgctgcagca ccttggccgc
     2761 ttcggctgcc agcacctgct cgcagcgcgc cagcatgaaa ggaatcgcct cacccatatg
     2821 accgatgatg atgttgagct tgcgatggcg gtccagcgca ccggagagca ccaggcgcaa
     2881 cgcctgcacc gcggtctcga tatgccatcc ccaggcggac gtcgccatgg caaagctcat
     2941 cattccaggc agcccatcat aatagacccg gcgcaccggt tcttcagcga tgccgggatg
     3001 aatgtagatc ggcacctcca aggccgcggc gcgcgccagg atcgggtcga acgagggatg
     3061 atcgaggcat tttcccgcgg tcagcccgtt gatcagcgca ccatggaagt gcaagtcgcg
     3121 cacacagcgt tccagctcat ccgccgcggc gtccggcgac agcatgggca gatgcgcgaa
     3181 gccggccagc cggccgggat gcgccgccac ggcgcgcgcc agggcgtcat tgtaatcgcg
     3241 cgcaagctga acgcccgccg tgccttccac cagatcggcg ccggggccgg acaccgacag
     3301 cacctgcacg ctgatgccgg cggcatccat gtcggcgatg cgcgtgggcc cgagatcggc
     3361 aagttcgcca cgcttgatcg cctgccccca gaccacgccg ggagcgggaa atccgcgtcg
     3421 ctggctcagc gcgggatcga tgcgtgccac cacgtcaggg gcgacgaagt gctcttccag
     3481 tgcgacgatg cgcatgtgac ggcctccctt gattggctgg aagtatggcc gagcatcgag
     3541 ggcggcgaaa cagccggggc cggaccaccc gcgggacggg accgccgcgg caggacggct
     3601 agcgccgcag ctccatctcg atcggtccct cgcgccggcc gtgggtgaac tggtccacca
     3661 tcgggttgcc gctctccagc agttccgacg ccggcccgct ccagacgatc ttgccgcgat
     3721 gcagcatcgc cgcgctatcg ccgatccgcc gggcactcgc catatcgtg
//
LOCUS       DASSZH010000512         2138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1358413, whole genome shotgun sequence.
ACCESSION   DASSZH010000512 DASSZH010000000
VERSION     DASSZH010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358413"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="VFN42_11190"
     CDS             <1..863
                     /locus_tag="VFN42_11190"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET8997221.1"
                     /translation="RLLEAAAALRLLERRGADRFGLGVLGAALAGNPGATAMIGHHPL
                     LYADLRDPVALLRGEHHDTALGRLWPYAAAGCKPIGATDVAAYSDLMAASQPLVAEDI
                     LDAYRLDRHRCLLDIGGGDGTFLTQVAARAAHLKLMLFDLPAVAERAQQHLAGSGLQD
                     RAQAYGGDVRAGNLPPGADIVSLVRVIHDHDDQDAMRILRSAWNALPPGGTLLLAEPM
                     AGTPAAPGVEAYFIFYLLAMGSGRPRRMEELTHMLQAAGFSQIRALPSRRPMLVRILV
                     ATRDERTAQP"
     gene            946..1884
                     /gene="bchC"
                     /locus_tag="VFN42_11195"
     CDS             946..1884
                     /gene="bchC"
                     /locus_tag="VFN42_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorophyll synthesis pathway protein BchC"
                     /protein_id="HET8997222.1"
                     /translation="MEALAVVLNRPESLSVDRVLLTSPGESDLVVDIAYSGISTGTER
                     LLWQGRMPAFPGMGYPLVPGYESVGRVVAAGPGHQSRIGETVFVPGATCFGEIRGLFG
                     GAASRLVVPASRAVAIDAELGERGILIALAATAQHAIADGHHLPELIVGHGVVGRLLA
                     RLVLAAGGQMPTVWETNPVRRDSPDDYRVLHPDEDSRRDYRCICDASGDAGLLDTLIS
                     RLGPGGEIVLAGFYADRLSFAFPTAFMREARLRIAAQWQPADLRAVQQLIARRALSLD
                     GLITHRVAAANAAAAYQTAFTDAACLKMILDWRASA"
     gene            1881..>2138
                     /locus_tag="VFN42_11200"
     CDS             1881..>2138
                     /locus_tag="VFN42_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8997223.1"
                     /translation="MNAPFDAALSQLRSEAAIEPDAPATGPVKKETQIIAIYGKGGIG
                     KSFTLANLSYMMAQQGKRVLLIGCDPKSDTTSLLFGGRSCPT"
BASE COUNT          336 a          730 c          715 g          357 t
ORIGIN      
        1 cccgcctgct ggaggcagcc gccgccctgc gcctgctgga acgccgcggc gccgatcggt
       61 ttggcctggg cgtgctgggc gcagcgctcg ccggcaatcc gggcgcgacg gcgatgatcg
      121 gacaccaccc gctgctgtat gccgacctgc gcgatcctgt cgcgctgctg cgcggcgagc
      181 accatgacac cgcgctcgga cgcctgtggc cctatgccgc cgcgggctgc aaacccatcg
      241 gcgccaccga cgtcgcggcc tacagcgacc tgatggccgc atcccagccg ctggtcgccg
      301 aggatatcct ggatgcctac cggctggatc gccaccgctg ccttttggac atcggcggcg
      361 gtgacggcac cttcctgacc caggtggccg cacgcgccgc acacctgaaa ttgatgctgt
      421 tcgacctgcc ggcggtcgcc gaacgggcac agcaacatct ggccgggtcc ggattgcagg
      481 accgcgccca ggcctacggc ggcgacgtcc gcgccggcaa tctgccgcct ggagccgaca
      541 tcgtatcgct ggtgcgggtc atccacgatc atgacgacca ggacgccatg cgcatcctgc
      601 gctcggcctg gaacgccctg ccgccgggcg gaacactact gctggcggag ccgatggcgg
      661 gaacaccggc tgccccgggc gtggaggcgt atttcatctt ctacttgctg gcgatgggca
      721 gcggacggcc gcgccgcatg gaggaactga cgcacatgct gcaggccgcc ggcttcagcc
      781 agatccgcgc gctgcccagc cggcgtccga tgctggtgcg cattcttgtc gcgacgcgtg
      841 atgaacgcac tgcacaaccg taaatcatag ttgacagttt aatatgtcaa tctaggatga
      901 cggaaagcgc aaacccaatg tcaccagttg acaggaggtg cggggatgga agcgttagcc
      961 gttgttctga acaggcctga aagtctctcg gttgaccggg tgctgctgac ctcgcccggc
     1021 gagtcggacc tggtggtgga cattgcctac agcggcatca gcaccggaac cgagcggctg
     1081 ctgtggcagg ggcggatgcc tgcgttcccc ggcatgggct atccgctggt gcccggctac
     1141 gagtcggtcg gccgtgtggt tgccgccggc cctggccatc aatcccgcat cggcgaaacc
     1201 gtcttcgttc ctggcgcgac ctgctttggt gagatccgcg gcctgttcgg cggcgccgcc
     1261 tcccgcctcg tggtgccggc cagccgagcg gtggcaatcg atgcggaatt gggggagcgg
     1321 gggatcctga tcgccctggc cgcgacggcg caacatgcca tcgccgacgg acaccacctg
     1381 cccgaactga tcgtcggtca cggcgtggtt gggcgcctgc tggcccgcct cgtgctggcg
     1441 gccggcggcc agatgccgac ggtctgggag accaacccgg ttcggcgcga cagcccggac
     1501 gactatcgcg tgctgcatcc ggatgaggac tcccgccgcg attaccggtg catttgcgac
     1561 gccagcggcg acgccgggct gctcgacacc ctgatcagcc ggcttggccc gggcggcgaa
     1621 attgtgctgg ccggatttta tgcggatcgg ctgtcctttg ccttcccgac cgccttcatg
     1681 cgcgaagcgc ggctgcgcat cgccgcgcaa tggcaacccg ccgatctgcg ggctgtgcag
     1741 cagctaattg cccgccgggc cttgtctctg gacggcctga tcacccaccg cgttgcggcg
     1801 gcgaacgccg cggccgccta ccagaccgca ttcaccgatg cggcctgcct gaaaatgatc
     1861 cttgattgga gagccagcgc atgaacgccc ccttcgatgc cgccttgtcg cagcttcgtt
     1921 cggaggcggc catcgagccc gacgccccgg cgaccggacc ggtgaaaaaa gaaacgcaga
     1981 tcatcgcgat ctatggcaag ggcggtatcg gcaagagctt cacgctggcg aacctgtcct
     2041 acatgatggc gcagcagggc aagcgggtgt tgctgatcgg ctgcgacccg aaaagcgaca
     2101 ccacgtcgct gctgttcggc gggcggagct gccccacc
//
LOCUS       DASSZH010000513         2761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_881365, whole genome shotgun sequence.
ACCESSION   DASSZH010000513 DASSZH010000000
VERSION     DASSZH010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881365"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..423)
                     /locus_tag="VFN42_11205"
     CDS             complement(79..423)
                     /locus_tag="VFN42_11205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8997224.1"
                     /translation="MEDDAPLRSMLAEFLRLEGFTVLETGAVADAKRVLSVRPNIQLV
                     LTDIQMPGGQSGIDLARYVQQTYPAVPILLVSGYFDQTAPEEFPLIRKPFGLQKLLSV
                     VQMLLSNSAPPV"
     gene            complement(938..1300)
                     /locus_tag="VFN42_11210"
     CDS             complement(938..1300)
                     /locus_tag="VFN42_11210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8997225.1"
                     /translation="MQRRSILIVEDDPDLADLLQTVLGDQYQTEWAADVPSALTCLGR
                     GSAEVVLLDCMVPAGSVTDVAEQARGRDCPVILMSGLPEMLNALAAFGYICLQKPFRM
                     GELLDAIESACVQQQQRR"
     gene            1653..2282
                     /locus_tag="VFN42_11215"
     CDS             1653..2282
                     /locus_tag="VFN42_11215"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010506667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET8997226.1"
                     /translation="MPNFALEKAAGGRVAGVDEVGRGPLAGPVVAAAVVFPTGVPRKL
                     AALLDDSKKLSADQRLAAYLALRASGKAEIGIGAASVAEIHRMNILRAALLAMRRAVA
                     RLPSEPDLALVDGNQPPPLLCPVRCVVGGDARCLSIAAASIVAKVVRDRAMTRLALRF
                     PGYGWETNVGYATEFHRDALRRLGATPHHRPTFFSVAQLSLNLEVAAAE"
     gene            complement(2444..>2761)
                     /locus_tag="VFN42_11220"
     CDS             complement(2444..>2761)
                     /locus_tag="VFN42_11220"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET8997227.1"
                     /translation="RSTAATEAMFLLLHLAADECGYRRLVWKCNALNAASRRAAERLG
                     FSYEGRHRMHMVVKGRQRDTDWYSIVGDEWPRCRDALRAWLAPGNFDAEGRPRRSLAS
                     LRS"
BASE COUNT          426 a          911 c          929 g          495 t
ORIGIN      
        1 tgtcgctggg catgctgcgg aaacggctgc gccgccggca ggttcgcatt cgtgcttgcc
       61 ggccgggtca cctgcggttc agaccggtgg ggcgctgttg gacaacagca tctgcacgac
      121 agacagaagc ttttgcaggc cgaacggttt cctgatcagg gggaattcct ccggagcggt
      181 ctgatcgaaa tatccgctga ccagcaggat cggaaccgcc ggataggtct gctgcacgta
      241 gcgcgccagg tcgatcccgc tctgcccgcc gggcatctgg atgtcggtga ggacgagttg
      301 aatgtttgga cgcaccgaaa gcacccgctt ggcgtctgcc accgctccgg tttccagcac
      361 ggtgaaacct tccagccgca ggaactccgc cagcatgctt cgaagcggcg cgtcgtcctc
      421 caccagcaat acggtggggg tggtctttgc cggttctgct gccgggagcg gatgctgaga
      481 ggccatccct gatgatttgc cccattccga tacatgattc ggctcacgat gtggtgagta
      541 cgctacccaa ccctctgatt tggcaggagt ctttcgcaca tggaggttgc gtccggcgcc
      601 gcggtcccgg cccgcctgca ccggcaggac ggcgcgggca gggcttggtt ggcctcatgc
      661 caggggaggg gcgacaggtg gcgggccgac ccggaacagc ggaatcgtgg cgcgtccgcc
      721 caatcgccct gctgccctgc cgtcgcgctt aggaattgcg ggcaaataag tgcttcaatc
      781 ggcgcgggcg cgacacacgt gtcatggcgg gcttggcccg gccgcccacg tctggccttg
      841 ttgtcagcag cgaagtcgtg ggtggccggc cttcgccggc catgacaaaa atgacgcgag
      901 ccgcctggag ctgactcggt tattgcgcga cggcgcctta gcgccgctgc tgctgctgca
      961 cgcaggccga ctcgattgcg tccagcaact cacccatcct gaatggtttc tgcaggcaga
     1021 tgtaaccgaa ggcggcaagt gcgttgagca tctccggcag gccggacatc aggatgaccg
     1081 gacaatcccg cccacgggct tgttcagcca cgtccgtgac gctgccggcc ggcaccatgc
     1141 agtccagcag aaccacttcc gccgagcctc tgccgaggca ggtgagcgcg ctcgggacat
     1201 cggcggccca ttcggtctga tactgatcgc ccagcacggt ctggagcagg tcggccaggt
     1261 cgggatcgtc ttcgacgatg agaatagaac ggcgctgcac tgccaagcgt cgcattcgcg
     1321 cctccctggg ttgccggttc ccgggcagga cttcacctcc cgatcgccag cgcccgggaa
     1381 ctagcgtcct ggcgatgcag gcgatgtagg gagccggggc agcagtctca acggtgcata
     1441 cggcgatgaa tataaatcca tgcttggcgg actgcttgct tgtctcatat cattactcgt
     1501 gtactggttc gtacatctta gttggggttg cctctgggct gccgcgccac gtcaccgatg
     1561 gtacggttcc ggcctgatcg ccaccgccgc ggcgcggaga gcgccgccac gaacctctcc
     1621 tccgactcgc gccgccctgc tatccatccc gcatgccgaa tttcgcgctg gagaaggccg
     1681 ccggggggcg cgtcgcaggg gtggacgagg tgggccgcgg gccgctcgcc ggcccggtcg
     1741 tggccgccgc cgtggtgttc ccaactggcg tgccgcgcaa gcttgctgcg ctgctggacg
     1801 attcgaaaaa gctgagcgcg gaccagcggc ttgctgccta tctcgcgctg cgtgcgtcgg
     1861 gaaaggcgga gatcggcatc ggtgccgcat cggtggccga gatccaccgc atgaacatcc
     1921 tgcgcgccgc gctgctggcg atgcgccgtg cggtggctcg cctgccctcg gaacccgatc
     1981 tggcgctggt ggacggcaac cagccgccgc cgctgctctg tccggtacgc tgcgtggtgg
     2041 gtggcgacgc gcgctgcctg tcgatcgccg ctgcctcgat cgtcgccaaa gtggtgcgtg
     2101 accgcgccat gacgcggctg gcgctgcggt ttcccggcta tggctgggaa accaatgtcg
     2161 gctacgccac cgagttccac cgcgacgccc tgcgccggct gggcgccacc ccgcaccacc
     2221 ggccaacatt tttcagcgtg gcgcagctca gcctgaacct ggaggtcgcg gcggcggagt
     2281 aatgccagcg gcccgaagcc cggactcgtt tgtgtcatgc ccgggcttgg cccgggcacc
     2341 cacgtcttac tgtgatgcat gcggcaaaga cgtgggtagc cgggccaagc ccggccatgg
     2401 cacggatggg ttagaacttc gagccgctgg tataagcggg gcttcagctt cgcagtgacg
     2461 ccaggctgcg ccgcggccgg ccctccgcat cgaaatttcc gggtgcgagc caggcgcgca
     2521 gcgcgtcgcg gcatcgcggc cactcgtcgc cgacgatgct gtaccagtcc gtgtcccgct
     2581 gccgcccttt caccaccata tgcatgcggt ggcgtccctc atagctgaag ccgagccgct
     2641 cggcggcgcg cctggaggcg gcgttgagcg cgttgcactt ccacaccagg cggcgatagc
     2701 cgcattcgtc cgcggccagg tgcagcagca ggaacatggc ttccgtcgcc gcggtgctgc
     2761 g
//
LOCUS       DASSZH010000514         5424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1409613, whole genome shotgun sequence.
ACCESSION   DASSZH010000514 DASSZH010000000
VERSION     DASSZH010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409613"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2279)
                     /locus_tag="VFN42_11225"
     CDS             complement(<1..2279)
                     /locus_tag="VFN42_11225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8997228.1"
                     /translation="MADPTTQASNEGLGGLGPNLGGIFDPGWYRARYRDVASSVVDPL
                     THFLNWGLDEGRDPNRYFDGAWYREHNADVAASGINPFLHYLQSGAAELRNPHPRFDA
                     IWYVDQHPEAARNPLLHHMLVGAAQGWLTEKPLDKRDFLPSTAAPFACPPDIAVDVVV
                     PVFRGLAETKRCLNSVLADRDRPAGRILVVDDHSPEPELVAWLDQLAAGGQIMLLRNR
                     RNLGFVASVNAGMRAAAPHDVVLLNSDTEVPAGWLRRLAAQAYAAPRIASVSPLSNNA
                     TICSYPGSEGSGTLPLGMTLAQVDAACRSVNAGRRVLLPTTVGFCMYIRRAALDHVGL
                     FDEETFGQGYGEENDFCLRAAAQGWSHVLACDTFVYHQGSVSFGANAAVLAERGIEVL
                     CRRYPGYRGLIANHVKQDAAGPFRFAVTAALLRQSGLPVILMVSHALGGGVQQHVDAL
                     VRRLDGAAHALLLRAGTRGPALTLPALPGHAEVSLPADRLGDLASLLQSCGVSRVHIH
                     HLLGMDLDVRKLLHRLEVPFDVTVHDYFALCPQVNLLPWKGAHYCGEPGPAACNACIA
                     DMPSHNARDILAWRSNFSWLFREAERVFCPSEDVRRRLERFGLAERAVVAPHEAAEAG
                     PWPLHPPKLRGGKLRVALLGVLAPHKGAHTVMSVVAAADPKAVEFHLIGYAEEGLPEG
                     AADGITVTGEYPEHELPALLAKVKPHVVWFPAQWPETYSYTLSAAIEAGLPIVASRIG
                     AFPERLDGRPLSWLLDPRAP"
     gene            2491..3858
                     /gene="gorA"
                     /locus_tag="VFN42_11230"
     CDS             2491..3858
                     /gene="gorA"
                     /locus_tag="VFN42_11230"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HET8997229.1"
                     /translation="MDFDLFVIGGGSAGVRCARIAAGHGARVGIAEERFWGGTCVNIG
                     CVPKKLLVQAGEYGAWADDAAGFGWTIQKGPHDWNKLIAAKDREINRLNSIYCKLLNN
                     AGASIFDARAVFLDPHTLDVGGKRITAERIVIATGGHPERPPIPGAELGIVSDDAFYL
                     PKMPKRVAIVGSGYIAVEFAGIFQALGAETHLIYRQPLPLRGFDQDLREGLAEALGAH
                     GIALHPGCRPTGVEAKGNRRVLRFGEGQHLDADLVFFATGRRPATRNLGLEQAGVSVN
                     AAGAVIVDNHLRTAQPHIYAMGDVTDRVNLTPVATAEGHALADSLFGGRPRTISLLNV
                     PSAVFSSPPIGTVGMTEEQAAAHGPVDVYLTKFTPMRHTLSGRNRKTLMKMVVNHADQ
                     KVVGVHMLGEDAPEIIQGIAIAVVMGATKADFDRTIGIHPTAAEEFVTLRTRTRTTGV
                     ARAAE"
     gene            complement(4078..4737)
                     /locus_tag="VFN42_11235"
     CDS             complement(4078..4737)
                     /locus_tag="VFN42_11235"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET8997230.1"
                     /translation="MTDLETAAPMRRQAENSLRNAILSGYYAAGERLKERQLMESLGV
                     SRTLLREALRQIEAEGLVTLVPNRGPVVSVLSYEDAEEIYEVRGILEAQACIGFVLRA
                     SSSNVRKLQAVFAKLKPAAEAGDVLRTLELSSEFYDVILEGSGNKILSSMLKLLHNRI
                     VLLRRTSMSEAGRLPETLDELTRMFNALCDRDEAAASKASIHHVRQASRAALRVMRKG
                     Q"
BASE COUNT          916 a         1799 c         1843 g          866 t
ORIGIN      
        1 ggggcgcgcg gatcgagcag ccaggacagc gggcgcccgt ccagccgttc cgggaaggcg
       61 ccgatccggc tggccacgat cggcagcccc gcttcgatcg cggcgctcag cgtgtagctg
      121 taggtctccg gccactgcgc cgggaaccag accacgtgcg gcttcacctt agccagcagg
      181 gcgggcagct cgtgttcggg gtactcgccg gtcaccgtga tgccgtcggc cgcgccctcc
      241 ggcaggccct cctcggcata gccgatcaga tggaattcca ccgcctttgg gtcggcggcc
      301 gcgactaccg acatgaccgt gtgggcaccc ttgtgcggtg ccagtacacc cagcagcgcc
      361 acgcgcaatt tcccgccacg cagcttgggc gggtgcagcg gccaggggcc cgcttcggcc
      421 gcttcgtgcg gagccacgac ggcgcgttcc gccaggccga accgctccag ccggcggcgc
      481 acgtcttcgc tggggcagaa gacccgttcc gcctcccgga acagccagga gaagttgctg
      541 cgccaggcca ggatgtcgcg cgcattgtgc gacggcatgt cggcgataca ggcattgcac
      601 gccgccggtc ctggctcccc gcaataatgc gcccccttcc agggcagcag attcacctgc
      661 gggcacagcg cgaagtaatc gtgcacggtg acatcgaacg gcacctccag gcggtgcagc
      721 agcttgcgga cgtccaggtc catgcccagc agatggtgga tatgcacccg gctgacgccg
      781 cagctctgca acaggcttgc caggtcgcca agccgatccg ccggaaggct cacctccgcg
      841 tggccgggaa gcgcgggcag ggtcagcgcc ggaccgcgcg tgcccgccct cagcagaagc
      901 gcatgggcgg caccgtccaa ccgcctgacc agcgcatcga catgctgctg cacgccgccg
      961 cccagcgcat gcgacaccat caggatgacc ggcaatccgg attgccgcag cagcgcggcc
     1021 gtgacggcga accggaacgg gccggcggca tcctgcttga cgtggttggc gataagtccg
     1081 cgatatcccg gatagcggcg gcacaggacc tcgatcccgc gttcagccag cactgcggca
     1141 ttggcgccaa agctgacgga gccctggtga tagacgaacg tgtcgcaggc cagaacatgg
     1201 ctccaaccct gcgcggccgc ccgcaggcag aagtcgttct cctcgccgta gccctgtccg
     1261 aacgtctcct cgtcgaacag ccccacatgg tccagggccg ctcggcggat atacatgcag
     1321 aagccgaccg tggtcggcag cagcacgcgg cggccggcat tcaccgaccg gcaggctgca
     1381 tcgacctgtg ccagggtcat gccgagcgga agcgtgccac tgccttcgct gcccgggtag
     1441 ctgcagatcg tcgcattgtt ggacagcggc gacaccgagg cgatgcgcgg cgccgcgtag
     1501 gcctgtgccg caagccgccg cagccagccg gcgggcactt ccgtatcgct gttcagcagc
     1561 accacgtcat gcggcgcggc cgcccgcatc ccggcattga ccgaggcaac gaagccgaga
     1621 ttgcggcggt tccgcaacag catgatttgt ccgccggctg cgagttgatc cagccaggca
     1681 accagttccg gctcgggcga atggtcatcc accaccagga tgcgcccggc cggccggtcc
     1741 cgatccgcca gcacgctgtt caggcagcgc ttggtttcgg ccaggccgcg gaacaccggc
     1801 accacgacat cgacggcgat atcgggcggg caggcgaagg gcgccgccgt ggagggcagg
     1861 aagtcgcgct tatcgagcgg cttctcggtc agccagccct gtgcggcgcc gaccagcatg
     1921 tggtgcaaca gcgggttgcg tgccgcctcg ggatgctggt cgacgtacca gatcgcgtcg
     1981 aatcgcggat gcgggttgcg cagttccgcg gccccggatt ggaggtagtg cagaaacgga
     2041 ttgattccgc tggccgcaac gtccgcgtta tgctcgcggt accaggcgcc gtcgaagtaa
     2101 cggttgggat cgcggccttc gtccaggccc cagttcagga agtgggtcag cggatcgacc
     2161 acggagctcg ccacatcgcg gtaacgcgcc cggtaccagc cgggatcgaa gattccgccc
     2221 agattgggcc ccaatccgcc gagcccctcg ttcgatgcct gggtggttgg gtcagccatg
     2281 gagaattcct gagacgcggg ggcggacgtc ttcgccacga gaagccgtga gcgttcccct
     2341 atcttgtcgt atgaagtcaa gccgcgcctt cccggaagga tgcggggagg caacaagctc
     2401 gcgtgagttc ggaccctggc ctgccgcggc ccatcttgcc gggcggggag cctggagaca
     2461 cagtccgcgc gcggtgaagg gatacatgcg atggattttg acctgttcgt gatcggcggc
     2521 ggctcggccg gggtgcgttg cgcccggatt gctgccggcc acggcgcccg cgtgggcatt
     2581 gcggaggagc gcttctgggg cggcacctgc gtcaatatcg gctgcgtgcc gaagaagctg
     2641 ctggtgcagg ctggcgaata cggcgcctgg gcggacgatg cggcaggctt cggctggacc
     2701 atccagaagg ggccgcacga ctggaacaag ctgatcgccg ccaaggatcg ggagatcaac
     2761 cggctgaaca gcatctactg caagctgctg aacaatgccg gcgccagcat cttcgacgcg
     2821 cgggcggtct tcctggatcc gcatacgctg gatgttggcg gcaagaggat caccgccgaa
     2881 cgcatcgtga ttgccacagg cgggcatccg gaacggccgc cgatcccggg cgccgaactg
     2941 gggattgtct cggacgacgc gttctacctg ccgaagatgc cgaaacgcgt ggcgatcgtc
     3001 gggtccggct acatcgccgt ggagttcgcc ggcatcttcc aggcgcttgg cgcggaaacc
     3061 cacctgatct accgccagcc gctcccgctg cgcggcttcg accaggacct gcgcgagggc
     3121 ctggccgagg cgctgggcgc gcacggcatc gcgctgcatc ccggctgccg tccgacaggg
     3181 gtcgaggcca agggcaaccg gcgcgtgctg cgcttcggcg agggccagca cctggacgcc
     3241 gacctggtgt tcttcgctac gggccgccgt cccgccacgc ggaacctggg gctggaacag
     3301 gccggggtga gcgtgaacgc cgccggggcg gtgatcgtcg acaaccatct gcgtaccgcg
     3361 cagccgcata tctacgcgat gggcgacgtg accgaccggg tgaacctgac cccggtcgcc
     3421 accgcggagg gccacgcgct ggcggattcg ctgttcggcg gccggccgcg cacgatctcc
     3481 ctgctgaacg tgccgtccgc ggtgttctcc agcccgccga tcggtaccgt gggaatgacg
     3541 gaggaacagg cggcggcgca cgggccggtg gatgtctacc tgaccaagtt cacgccgatg
     3601 cgtcacaccc tgtcggggcg caaccgcaag acgctgatga agatggtggt gaatcacgcg
     3661 gaccagaaag tggtgggcgt gcacatgctg ggcgaggatg cgccggagat catccagggc
     3721 atcgcgatcg ccgtcgtcat gggagccacc aaggcggatt tcgaccgcac catcggcatc
     3781 cacccgaccg cggccgagga gttcgtcacc ctgcgcaccc gcacccgcac gaccggcgtg
     3841 gccagggccg cggagtagga aagcggggag gtcactccaa gccggcaaac gagatcgcgg
     3901 atcgtgaaga ccgccggtgt gaatcggctc ggcgtttgac ccctctatcg gcgtcagatg
     3961 gtgacccacc gggcgaacgc ctatgccatt gatatcgggg cggattcctc tgtcgatggc
     4021 ggtcagaatt ggacgccgaa atcggtcaag attgaaggcc gaaacacagc gtgcttctca
     4081 ctggcccttg cgcatgaccc gcaaggccgc gcgcgacgct tggcgcacat ggtggatcga
     4141 tgccttgctg gccgccgctt catcccggtc gcaaagcgcg ttgaacatcc gggtgagttc
     4201 gtcgagcgtt tcgggcaggc ggccggcttc cgacatcgaa gtccggcgca gcaggacgat
     4261 acggttgtgc agaagcttca gcatcgaact caggatcttg ttgccgcttc cttccaggat
     4321 aacgtcgtag aattcgctgc tcagctccag ggtgcgcagg acgtcgccgg cctccgcggc
     4381 gggcttaagc ttggcgaaaa cggcctgcag cttgcggacg ttgctgctcg acgcgcgcag
     4441 aacgaagccg atgcaggcct gcgcctcgag gatgccgcgg acctcgtaga tttcctcggc
     4501 gtcctcgtac gacaggaccg agacgacggg gccgcggttg ggaaccagcg tcaccaaccc
     4561 ctcggcctcg atctggcgca gcgcctcgcg cagcagggtc cggctgacgc cgaggctctc
     4621 catcagctgc cgctccttga gccgctctcc ggcagcgtag tatccggaca gtatggcatt
     4681 gcgcagactg ttctccgctt gtctgcgcat tggcgccgcg gtctcgagat ccgtcattgg
     4741 gtcagctctc ctaaaggggc ggcacgggta cgcccttggg cccgaacaga ttgcatcatc
     4801 cgttgaacgc aaggcgtgga accccctcct tcctgaagat cacggtgtcc gaaaggaacg
     4861 gtgtcgccgc ctcagcgctc acggcgagac gacggagcca gccggccttc ttcattgcat
     4921 tgaaggtgcc ggtggtgagc aggatcgaaa cgacgccggt gaagagggag ttgtcgcgcc
     4981 ctttggcgaa gccgaagcac tgcccgcctg cgttctcgcc cgcccgcagt gtggcgaacc
     5041 ggtgcgggga tgccggcgga ggtgcagcag attgccgtcc ggatgattga acggtaggga
     5101 aaggctgaac cttcggccga tcctggcttc ggtcaagcgg ttcaggacct gagcgttgcc
     5161 ccccaggttc atggctccaa gccgatcctc ggcaccgagg ccaccccggt tcggattctc
     5221 cgagcggtgc tcccggcggg cttccatact tactgccctt gtccttgccg ctcagcctcc
     5281 tggcgctccg ccgcttcggc ggccacgacg ctcggccgtg ccccgatacg cgcgagatag
     5341 gccttgagat gaggccaagg tccgagatcg atatcgtgga gtctggtcca catcacaatg
     5401 gtgtagcagt aggcgtcagc gacg
//
LOCUS       DASSZH010000515         2210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1120258, whole genome shotgun sequence.
ACCESSION   DASSZH010000515 DASSZH010000000
VERSION     DASSZH010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120258"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /gene="rfbC"
                     /locus_tag="VFN42_11240"
     CDS             complement(<1..573)
                     /gene="rfbC"
                     /locus_tag="VFN42_11240"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HET8997231.1"
                     /translation="MRFTPVALGGPMIIDLDAHEDERGSFARTFCAAEFAQAGLPACF
                     VQCSLSRNPRRGTLRGLHYQAPPREEGKLVRCTRGAIWDVAVDLRRNSVTCGRWVAEE
                     LSAENQRALWVPPGFAHGFVTLCDDTDVFYQMTAPYCPELSAGVRWDDPAFGIAWPVR
                     DPWLSERDRRYPDWVTAPRRCGTLLEAAALP"
     gene            complement(573..1805)
                     /locus_tag="VFN42_11245"
     CDS             complement(573..1805)
                     /locus_tag="VFN42_11245"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET8997232.1"
                     /translation="MTQRICRFCAAPLTESFADLGMTPLSNAFVPPERAAAPERFYPL
                     HAFVCSLCRLVQLDQAEPPQAIFRDYPYFSSYAESWLRHAERYAAAMMERFALGRSSR
                     VVEVASNDGYLLQFFHRAGIPVLGIEPAENVACVARQRGIATDAAFFGCVTARRLRTQ
                     GIEADLLVANNVLAHVPALNDFVAGLGTLLHPDGVLTVEMPHLLRLIEGSQFDTIYHE
                     HFSYFSLLAAQRIFAAHGLRVFDVAQLPTHGGSLRLFLCHAACGAHKPHDTVDAVLAE
                     ERAAGLDGPHAYRRFAEQMVRSKCGLLDFCIAARRAGQRMVGYGAPAKGNTLLNYCGI
                     GPEFLPFTVDRNPHKQGMLLPGTRIPVRAPDSVMQARPDYLLILPWNLRDEVMAQMAA
                     IRDWGGRFVLPIPSVQVL"
     gene            1915..>2210
                     /locus_tag="VFN42_11250"
     CDS             1915..>2210
                     /locus_tag="VFN42_11250"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HET8997233.1"
                     /translation="MSTPKIAFIGTGGTIASLGRHRLDLQDYGAAGKVMHAEEILAAF
                     PEVATVADVFPVRYRNVVSPAIDFTDWKALVLLCDRLVAEHPDLAGIVIGHGTAT"
BASE COUNT          382 a          748 c          755 g          325 t
ORIGIN      
        1 cggcaacgcg gccgcctcaa gcaacgtgcc gcagcgcctg ggcgcggtga cccagtcggg
       61 atagcggcgg tcgcgttccg acagccacgg atcgcgcacc ggccaggcaa tgccgaaagc
      121 cggatcgtcc catcgcacgc cggcactcag ttccgggcaa tagggcgcgg tcatctggta
      181 gaagacatcg gtgtcgtcgc acagcgtgac gaagccgtgc gcgaagcctg gcggcaccca
      241 aagggcgcgc tggttctccg cactcagttc ctccgccacc cagcgcccgc aggtgacgga
      301 gttgcgccgc agatccaccg cgacgtccca gatcgcgccg cgtgtgcaac gcaccagctt
      361 gccctcttcg cgcggcggtg cctggtaatg cagcccccgc agcgtgccgc ggcgcgggtt
      421 gcgcgacagg ctgcactgca cgaagcaggc cggcagtccg gcctgggcaa actcggcggc
      481 acagaaggtc cgtgcgaagc tgccgcgttc gtcttcgtgc gcgtccaggt cgatgatcat
      541 cgggccgccg agcgccacgg gggtgaagcg catcacagca cctgcacgct ggggatgggc
      601 agcacgaaac ggccacccca gtcgcggatg gctgccatct gcgccatcac ctcgtcgcgc
      661 agattccagg gcaggatcag caggtaatcg ggccgcgcct gcatgaccga gtccggtgcg
      721 cgcaccggga tacgggtgcc gggcagcagc atgccctgct tgtgcggatt gcgatccacc
      781 gtgaagggca ggaactccgg gccgatgccg cagtagttca gcagcgtgtt gcccttggcg
      841 ggggcgccat atcccaccat gcgctggccg gcacgccgcg cggcgatgca gaaatccagc
      901 aggccgcatt tgctccgcac catttgttcg gcgaagcggc gatacgcatg cggaccgtcc
      961 agcccggcgg cgcgttcctc tgccagcacc gcatccaccg tgtcatgcgg cttgtgcgcg
     1021 ccgcacgcgg catgacacag gaacagccgc agcgagccgc catgcgtcgg cagttgtgca
     1081 acgtcgaaca cgcgcaggcc atgcgcggcg aagatccgct gcgcggccag cagcgagaaa
     1141 tacgaaaagt gctcgtgata gatcgtgtcg aactggctgc cctcgatcag ccgcagcaaa
     1201 tgcggcattt cgaccgtcag cacgccgtcc ggatgcagca gcgtgcccag cccggcgacg
     1261 aagtcgttca atgccggcac atgcgccagc acgttgttgg ccaccagcag gtcggcttcg
     1321 atgccctgtg tccgcaaacg ccgcgcggta acgcagccga agaatgcggc atcggtggcg
     1381 atgccgcgtt gcctcgccac gcaggccaca ttctccgccg gttcgatgcc cagcaccggg
     1441 atgccggcgc ggtggaagaa ctgcagcagg tagccgtcgt tgctggccac ttccaccacg
     1501 cgcgacgacc gtcccagcgc gaagcgctcc atcatcgccg ccgcgtagcg ttcggcgtga
     1561 cgcagccagc tttcggcgta ggaggagaaa tacggatagt cccggaagat cgcctgcggc
     1621 ggttccgcct ggtcgagctg caccaggcgg cagaggctgc aaacgaaggc atgcagcgga
     1681 tagaaacgct cgggcgccgc ggcgcgctcg ggcgggacga aggcgttgga cagcggagtc
     1741 atgcccagat cggcgaagct ctcggtcaag ggcgccgcgc agaagcggca gatccgctgg
     1801 gtcatcggtg ctcgcgtccg gaatggcgtc ccgcagtctg ccgtggctgc attgccgagg
     1861 cgttaacgcg gcggccgagc tttcatcccg cagcgagtgc ggtagagtcc cggcatgagc
     1921 acaccgaaaa tcgccttcat cggcaccggc ggcaccatcg cttcgcttgg ccggcaccgg
     1981 ctggacctgc aggattacgg agcggcgggc aaggtgatgc acgccgagga gatcctggcg
     2041 gcgttccccg aggtggcgac ggtggccgac gtgttccccg tgcgctaccg caacgtggtc
     2101 agtcccgcga tcgacttcac cgactggaag gcgctggtgc tgctgtgcga ccggttggtg
     2161 gcggagcatc ccgaccttgc cggcatcgtc atcggccacg gcaccgcgac
//
LOCUS       DASSZH010000516         5141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_97283, whole genome shotgun sequence.
ACCESSION   DASSZH010000516 DASSZH010000000
VERSION     DASSZH010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97283"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(475..1086)
                     /locus_tag="VFN42_11255"
     CDS             complement(475..1086)
                     /locus_tag="VFN42_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997234.1"
                     /translation="MNTAPLLLSVLSNTPGGAAPSVSNHIGPLLMLLRLLIGTGAIIC
                     ILICIGHMLRRAVGPEPRFRQQAAPDHHQETGAERALVSPRLRGNRSISPAHWRAAGT
                     GTDDSNLLMTIRSATRTSPSRPAAGSPALRRDLGLVSGIVSQQQRKEWADIIAAFRRE
                     FRHAGLSAEREPLALAHDRGQGDRGPVFHADTQRTAESAEHLH"
     gene            1194..2693
                     /locus_tag="VFN42_11260"
     CDS             1194..2693
                     /locus_tag="VFN42_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HET8997235.1"
                     /translation="MKPPIRIAVLHFSHETVTFLPNDTTLADFVYPGSPAAGEALLGT
                     DPRGYMGGFVKLAREYEAVELVGIASPLWPRTGTGSGWITTEAFEIFTGRMIEDLQLQ
                     GPFDGAYLCLHGAAAVRGVPRPEAELARRVRAAIGPAAFIAGTFDPHGNEDEQFLRHA
                     NMAFAGKYFPHYDSYLQGERAARCLVRAIRGDYAPVSATRKVPIISPTVFQWTGASPW
                     MDLVQRALTWEAREPDLFVNFFFGFPWADVPDVGMTFQVLANGKPELAREVADDMAES
                     AWRQRAALLNTAKINSIDEGVAAAKRAVADGTTPVVLADHSDRSGYATWLLRAVIEQG
                     LSRVLIGTVADARLVAELLAKGAQPGDAVDAEVGGLVDDSAGLPVRIQGSIVGISQAS
                     GRVKGNTWFHIGFGDGNVLIVSPYLAQIIEPATFTGLGLDLAAFDVFAIKSRVHFRRG
                     FDDSGFARMILLVEPDQPFLGTVRLEGLPYRNVDLKAFYPYGEPQAPDV"
     gene            complement(2815..3150)
                     /locus_tag="VFN42_11265"
     CDS             complement(2815..3150)
                     /locus_tag="VFN42_11265"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HET8997236.1"
                     /translation="MKRGDVVTVAVSGDYGKPRPALVIQSDAFAALPSVTVLRLSSEI
                     IPAHLTRITIEPTSANGLRFRSQVMLDKAISVPRAKIGKTVGCLDTATMRAVSRGLIA
                     FFDLDEMLA"
     gene            complement(3156..3449)
                     /locus_tag="VFN42_11270"
     CDS             complement(3156..3449)
                     /locus_tag="VFN42_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997237.1"
                     /translation="MPRTTQHPKETSFNMRIDPQLKAAFTKATEAEDKPAAQVVRSFM
                     RAYVRRRERLAFEAEARRQSRLIAERARDPQSDEAHVMREIEAELADESFWSE"
     gene            3628..4563
                     /locus_tag="VFN42_11275"
     CDS             3628..4563
                     /locus_tag="VFN42_11275"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HET8997238.1"
                     /translation="MALLIVDLDKHIGNAQAWRETFAEQLPGLEIRIWPEAGDLAEID
                     YLAFMHPDFDALPSFPNLKAMFSRSAGVESFVNHPRLPKARLCKIEPPGGDPMMTEYV
                     VMHVLRLHRDMPGYAAAQARKEWRRTPIVRPEQRRVGFLGFGMMAKAPALVLQSLGFP
                     VSAWVRNPRQEGGVAIYHGLDQLEAFLAQTDIAVCLLPLTRETEGIFCARTFAMMPRG
                     AMLVNVGRGKHVVEADLIEALDSGQLAYAALDALWPEPLPPSSPLWLHPKVTVMPHVA
                     RRPTVAQLVTEIAANIRSLEAGGRALQEVDIARGY"
     gene            complement(4586..>5141)
                     /locus_tag="VFN42_11280"
     CDS             complement(4586..>5141)
                     /locus_tag="VFN42_11280"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET8997239.1"
                     /translation="LRLLLTGLLEPELLAHASAPRLTVAATDVATGEAVLFDNAAVTV
                     DVLLASACLPFVFPAVEIDGRAYWDGGYAGNPPLGPLLRPELPDELILVRAQPARRPG
                     VPATPMEITNRLNEIACQNVLAAELAALPPAVQIRSYDADEALLDLPISSKFNGEAEF
                     LSELFQAGRAAAGAQAEACRAAAD"
BASE COUNT          847 a         1634 c         1783 g          877 t
ORIGIN      
        1 cgctgggggt gaagggggcc gggcaggcgg gcgcaatggc cgcgccgcag gcgatcgtct
       61 gcgcagtgct ggacgcgctc gcgccgctcg gcgtcgcgca tctggacatg ccggtcacac
      121 cggaacgggt gtggcgggcg atccaggagg caaagccgcg ctgaagcagg cagggtgccc
      181 gtgcggaatt gccgttggaa acgggcatct atggctatac gggtgatgct ggagatgggc
      241 ccagcacagt attccgcgca gagacgcagg ggaccgtaga gtccgcagaa agccacggcg
      301 tcatgcaagc ccgacgtgat gatcacgcac cggcggcagt atcacgtcgt ggcgggcgca
      361 ggcccgccag ccacgaattt gccgcgtgtg cctgcggagt cgtggatccc gcccttcgcc
      421 ggcatgacgg agtgcagcac gccgaagagt cgagcttatc gcggtggtat tattctagtg
      481 cagatgctct gcggactctg cggtcctctg cgtgtctgcg tggaatacgg ggccgcgatc
      541 cccctgtcct cggtcatgtg ccagtgccag cggttcgcgt tccgcgctca aaccggcatg
      601 gcgaaattcc cgccggaacg cggcaatgat gtcggcccat tcctttctct gctgctggct
      661 gacgatgccg gacaccaagc cgaggtcgcg gcggagcgct gggcttcccg cggcgggcct
      721 ggacggcgat gtccgcgttg ccgagcggat cgtcatcagg aggttgctgt catcggtccc
      781 agtccccgct gcgcgccaat gggcggggct gatcgaccgg ttgccgcgca ggcgcggcga
      841 gaccagcgcc cgttcggcgc cggtctcctg gtggtggtcg ggagcggcct gctgccggaa
      901 ccggggctcc ggaccaacag cacgccgcag catatggcca atacaaatca ggatgcagat
      961 gatagcgccc gtgccgatca gcagccgcag gagcatcaga agcgggccga tgtgattgga
     1021 cacggaaggg gcggccccgc ccggggtgtt ggaaagaaca gagaggagga ggggcgctgt
     1081 gttcatggaa cgtatataga acataagtcg gcggcgatgt caagggaatt ttttctgaga
     1141 ggcggaagcg cggcgaccgg cctatcctcg tcgcagcgaa cgaggaccgc atcatgaaac
     1201 cgccgatccg catcgccgtg ctgcatttct cccatgaaac ggtgacgttc ctgcccaacg
     1261 acaccaccct ggcggacttc gtctatcccg gctcccccgc cgcgggcgag gcgctgctgg
     1321 gcaccgatcc gcgcggctac atgggcggct tcgtgaagct ggcgcgcgag tacgaggcgg
     1381 tggagctggt gggcatcgca tcgccgctgt ggccgcgcac cggcaccggc tccggctgga
     1441 tcaccaccga ggcgttcgag atcttcaccg gccgcatgat tgaggacctc cagcttcagg
     1501 ggccgttcga cggcgcctat ctctgcctgc acggcgctgc cgccgtgcgt ggcgtgccgc
     1561 ggccggaggc ggagctggcc aggcgcgtgc gcgccgcgat cggccccgcc gcgttcatcg
     1621 ccggcacctt cgatccgcac ggcaacgagg acgagcagtt cctccgccat gccaacatgg
     1681 ccttcgcggg gaagtacttt ccgcattacg acagctacct gcagggcgag cgcgcggcgc
     1741 gctgcctggt gcgcgcgatc cgcggcgact acgcgccggt gtcggcgacg cggaaggtgc
     1801 cgatcatctc gcccaccgtg ttccaatgga ccggcgcctc gccgtggatg gacctggtgc
     1861 agcgggcgct gacctgggag gcgcgcgaac cagacctgtt cgtcaacttc tttttcggct
     1921 tcccgtgggc ggacgtgccg gatgtcggga tgaccttcca ggtgctggcc aacggcaagc
     1981 cggaactggc acgggaagtg gcggacgaca tggcggagag tgcatggcgg cagcgtgcgg
     2041 ccttgctgaa cacggcgaag atcaattcga ttgatgaagg cgtggcggca gcgaagcgtg
     2101 cggtggcgga tggcacgaca ccggtggtgc tggccgacca tagcgaccgc tcgggctatg
     2161 ccacctggct gttgcgtgcg gtgatcgaac aagggctgtc gcgcgtgctg atcggcaccg
     2221 tggcggatgc gcggctggtc gccgagctgt tggcgaaggg cgcgcagccg ggcgatgcgg
     2281 tggatgccga agtcggcggg ctggtcgatg actcggcagg attgccggtg cggatccagg
     2341 gaagcatcgt ggggatcagc caggcgtcgg gacgggtgaa gggaaacacc tggttccata
     2401 tcggcttcgg cgacggcaat gtgctgatcg tcagcccgta cctggcgcag atcatcgagc
     2461 ccgcgacctt caccggcctg ggcctcgacc tggcggcatt cgacgtcttt gccatcaagt
     2521 cccgcgtgca tttccggcgt ggcttcgatg acagcgggtt cgccaggatg atcctgctgg
     2581 tggagccgga tcagccgttc ctcggcaccg tacgattgga gggattgccg tatcggaatg
     2641 tggatctgaa ggcgttctat ccgtatgggg agccgcaagc tcccgacgtc tgattggtgc
     2701 gccggagcgt ttgcgttcag ggacgggata tcccgcacac cagatctggc gatgggccgg
     2761 gagagttccg aaggcgacct cggcgccgcg atcggcgttc ttcgtcaatg gcgattacgc
     2821 cagcatctcg tccagatcga agaacgcgat aagccccctg ctgaccgcac gcatggtggc
     2881 cgtgtccagg cagccgaccg tcttgccgat cttcgcacga ggcacggaga tcgccttgtc
     2941 gagcatgacc tgtgagcgga agcgcaatcc attggcagaa gtgggctcaa tggtgatacg
     3001 ggtcaaatgg gccgggatga tctcactcga cagccgtaac actgtcaccg agggcaaggc
     3061 agcgaaggca tccgattgaa taacaagcgc cggacgcggc ttgccgtaat cgccggacac
     3121 tgccacggtc acgacatcgc cccgtttcat gtcagctatt cgctccaaaa gctctcgtcg
     3181 gcgagttcgg cctcgatctc gcgcatcaca tgggcctcat cgctttgcgg atccctggcg
     3241 cgttccgcaa tgagccgtga ttgccgccgg gcttccgctt caaaggcgag acgctcgcgc
     3301 cgccggacgt aagcccgcat gaaactccgg acgacttggg cggccggctt gtcttctgcc
     3361 tcggtagcct tggtgaacgc cgccttcaat tgcggatcaa ttcgcatgtt gaagctggtt
     3421 tccttgggat gctgcgtcgt ccggggcata gaaaaactcc gtactggcgt gatgcaggta
     3481 taatgtcgtc acattatgcc ttcaaggcac cccgggacat ccctggcctg gggcacacca
     3541 cggacctgcg ggtcaggatc aatagatcgt ctatcgcgcc ccggccgctt cgctacactg
     3601 actctcccgc acccccagga gccgtccatg gcgctgctca tcgtcgatct cgacaagcat
     3661 atcggcaacg cccaggcatg gcgcgagacc ttcgccgagc aattgcccgg gctggaaatc
     3721 cgcatctggc cggaggcggg cgatctggcg gagatcgact atctcgcctt catgcatccc
     3781 gacttcgacg cccttcccag ctttcccaat ctgaaagcca tgttcagccg ctcggccggt
     3841 gtcgagtcct tcgtcaacca tcccaggctg ccaaaggcca ggctgtgcaa gatcgagccg
     3901 ccgggcggcg atcctatgat gacggaatat gtcgtcatgc acgtgctgcg cctgcatcgc
     3961 gacatgcccg gctacgcggc ggcgcaggct cgcaaggagt ggcgccgcac gcccattgtc
     4021 cggcccgagc agcggcgcgt gggcttcctc ggcttcggca tgatggcgaa agcgccggcg
     4081 ctggtgctgc agtcgctagg gtttccggta tcggcctggg tgcgcaatcc gcggcaggaa
     4141 ggcggcgtcg cgatctatca cggtctcgat cagctcgaag ccttcctcgc ccagaccgat
     4201 atcgcggtct gcctgctgcc gctgacacgg gaaaccgagg gcatcttctg cgcgcgcacc
     4261 ttcgcgatga tgccgcgcgg cgcgatgctg gtgaatgtcg ggcgcggcaa gcacgtggtg
     4321 gaggcggacc tgatcgaggc gctcgattca ggccagttgg cctacgccgc gctggacgcg
     4381 ctgtggcccg agccgctgcc gcccagcagc ccgctttggc tgcaccccaa ggtcaccgtg
     4441 atgccgcacg tggcgcggcg gccgacggtg gcgcagcttg tgacggagat cgcggcgaat
     4501 atcagaagcc tggaggcggg cgggcgggcc ttgcaggaag tcgatatcgc caggggctac
     4561 tagcccggcc gggtgagcat gccgctcaat ccgctgccgc gcggcaggct tcggcctgcg
     4621 ctccggccgc ggcgcgcccg gcctggaaca attccgacag gaactccgcc tcgccgttga
     4681 acttcgagga gatcggcagg tccagcagcg cctcgtccgc atcgtagctg cggatctgca
     4741 ccgccggcgg cagcgccgcc agctccgccg cgagcacgtt ctggcaggcg atctcgttca
     4801 gccggttggt gatctccatg ggcgtggccg gcacgccggg gcggcgggcc ggctgggcgc
     4861 gcaccaggat cagttcgtcg ggcagctccg gccgcagcag cggacccagc ggcgggttgc
     4921 cggcatagcc gccgtcccag taggcgcgtc cgtcgatctc gacggccggg aacacgaagg
     4981 gcaggcaggc ggaggccagc agcacatcca cggtgaccgc ggcgttgtcg aacagcaccg
     5041 cttctccggt tgccacgtcc gtcgcggcga cggtcaggcg tggcgcggag gcgtgtgcca
     5101 gcaactccgg ttccagcagt ccggtgagca gcagccgcag c
//
LOCUS       DASSZH010000517         5283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_711163, whole genome shotgun sequence.
ACCESSION   DASSZH010000517 DASSZH010000000
VERSION     DASSZH010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5283
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711163"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VFN42_11285"
     CDS             complement(<1..951)
                     /locus_tag="VFN42_11285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET8997240.1"
                     /translation="MAIRDETRMLLESVGRLFEDKCTKQVIDRAESGVFAADLWRAIA
                     ETGVPLAALPESAGGADAEWADLYAVLRVAGRYAAPVPLAETMLAGWIAASSGLELSD
                     GPMSVGPVRASDTLTLQRDGNGWRLSGTASRLPYARDADRIVLLADGPDGEMAVSLQA
                     TQGADATQGRNMAGEPRDTLRFDALRLPADAAAPAKDGVSRASLYRRGALARATMMSG
                     ALERAMDLAVSYAQERQQFGRPISKFQAVQQNLAVLAGQTAAAVAAANLGIEALSKQD
                     PEMEEFRIAVAKTRVGEAATLGGELAHQVHGAIGFTKEYSL"
     gene            complement(1128..2276)
                     /locus_tag="VFN42_11290"
     CDS             complement(1128..2276)
                     /locus_tag="VFN42_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET8997241.1"
                     /translation="MAVPMFRFDPMELPPEALALRTEVRAFIREHEHLMGLSRGEFSR
                     DFSRAMGRRGWIGMTWPKQYGGHERSAFERYVVIEEMLVAGAPLSAHYTGDRQSGPNI
                     LRFGTEAQKQFFLPQIAAGELTFCIGMSEPNSGSDLAGTRTRAVKVDGGYRINGSKLW
                     TSNAHRADYAILFCKMAQQGQEEVDRHAGATQFLLDLKTPGIEIRPVLNLLGQHHFNE
                     IFLTDVFVPDWLLLGKEGEGWKQVTSELSFERSAPDRFLAFFQMLREMVRMAGANPDK
                     ATAAALGRLIAHLATLRSMSLSIAGMLQEGLQPNNESAVVKDLGTRYEQDIPVVARQI
                     FPAEPELEAVETYVSMIARTTMNAPRLSIQGGTREILRGIIARGLGLR"
     gene            2419..3648
                     /locus_tag="VFN42_11295"
     CDS             2419..3648
                     /locus_tag="VFN42_11295"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET8997242.1"
                     /translation="METNAADVDFLSGVRVVDFTQFEAGPSCTEALAWLGADVVKIEN
                     PRMGDPGRRLRPGEPDNDPYYFHVFNANKKSITVNLKSPRGLQLVQDMLRKADVCVEN
                     FAPGTIERLGLGYDAVRALNPGIIYASVKGFGEGSPYEKNLAFDMIAQACGGTFSVTG
                     EADGPPTRPGMSLGDTGTGMLMAITVLGALHKRAATGKGCRLQVAMQDAMLHYMRINF
                     ATQALTGRPAQRGGSKVVGINNAPMGLYPCKPGGPNDYLYIMTSRANPEHWDRLLKLI
                     GREDLIGDPRYATPALRVQHEAEVDEIIAAWTRQHTKHEAMDAIGSVGVPAGAVLDTM
                     ELQNDESFARRGIMQTIHHPAHGDFKMPAWPVRIDGKPPRAVTSPVLGQHTGEVLGAW
                     LGMSADDVAKLRDEGAV"
     gene            complement(3759..4340)
                     /locus_tag="VFN42_11300"
     CDS             complement(3759..4340)
                     /locus_tag="VFN42_11300"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET8997243.1"
                     /translation="MMHTTDVLNLFAEHRGDAIVIPGRGGRHWVNMSETAFDLPMGDP
                     AMGGHAGFGLGVALAQPNRKVVLFDSEGDVLMSLGMLATAAEQAPANFYHFMLDNECY
                     ATTGGQPVPNAKNVDYTAIARGAGYPRALAFAELEALRRELPGILRQPGPVFVALKVL
                     PEIENTPIGGRVRWRKRTADKVVQDLRTALGVG"
     gene            complement(4355..4873)
                     /locus_tag="VFN42_11305"
     CDS             complement(4355..4873)
                     /locus_tag="VFN42_11305"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET8997244.1"
                     /translation="MADTAAAALSPETVMQQMKRNGVTDVVWLPDSETNWLFLLMQAD
                     PGLRLVGVTREGHAASIAAGLYAGGRKPMILIQNTGMMESGDSIRGWLMGLQVPVVLM
                     VGYRGYTRHGVNSDTAATYTERFLMAFNLSYYLVENDADADRISIAFQEAERTRKPVV
                     VLVGDEYHGFNR"
     gene            complement(4933..>5283)
                     /locus_tag="VFN42_11310"
     CDS             complement(4933..>5283)
                     /locus_tag="VFN42_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997245.1"
                     /translation="ESGATTPLGQAMAHDHRRILATGIARLRGKIKYRPVVFELMPPA
                     FTLGQLQRAVEALAGRRLHKQNFRRLMEQQELVEETGAVATETGGRPAKLFRFRRAVL
                     AERAVAGTKLPLAR"
BASE COUNT          882 a         1781 c         1733 g          887 t
ORIGIN      
        1 cagcgaatat tccttggtga agcctatggc gccgtgcacc tgatgcgcca gttcgccgcc
       61 gagtgttgct gcttctccca cacgcgtctt cgccactgca atgcggaatt cctccatctc
      121 tgggtcttgc ttggacagcg cttcgatgcc gaggttcgcg gcagcaacgg ccgcggcggt
      181 ctgcccggca agcacggcca gattctgctg caccgcctgg aacttcgaga tcggccggcc
      241 gaactgctgg cgttcctggg catagctgac ggccaggtcc atcgcgcgtt ccagcgcacc
      301 cgacatcatc gtggcgcgcg ccagcgcacc gcggcggtac agcgacgcac gggaaacgcc
      361 gtccttggcc ggcgccgcag catccgcggg caaccggagt gcgtcgaagc gcagcgtgtc
      421 gcgcggttct cctgccatgt tacggccttg cgtggcgtcg gcgccttgcg tggcttgcag
      481 cgataccgcc atttcgccgt cgggaccgtc ggccaatagc acgatgcggt cggcgtcgcg
      541 cgcgtagggc agccggctgg cggtgcccga caggcgccag ccattgccgt cgcgctgcag
      601 cgtcaacgtg tcggaggccc gcacgggacc gacgctcatt ggcccgtcgc tgagttccag
      661 gccggaactg gcggcaatcc agccggcaag catggtctcc gccagcggaa ccggcgctgc
      721 atagcggccg gcgacccgca gcacggcata caggtcggcc cattcagcat cggcgccgcc
      781 ggccgattcc ggcaaggcgg cgagcggcac gccggtttcc gcgatggcac gccagaggtc
      841 cgcggcaaac accccggatt cggcgcggtc gatcacctgt ttggtgcact tgtcctcgaa
      901 cagccgcccg acgctttcca gcagcatccg ggtttcgtcg cggatcgcca tgttggtgtt
      961 ccctcaatgc ttcgagcggc ggccgggcaa cctcgtgtca tggccgggct tgacccggcc
     1021 acccacgcct tgctgcggcg gatgtggaaa gacgtgggtg cccgggtcaa gcccgggcat
     1081 gacacagaag gggccgagca tgacagcgca gcccgctgga ttgcgcatca ccgcaacccc
     1141 agcccgcgcg cgatgatgcc gcgcaggatt tcccgtgtgc cgccctggat cgacagccgc
     1201 ggcgcgttca tcgtggtgcg cgcgatcatc gagacgtacg tctccaccgc ctccagctcc
     1261 ggttccgccg ggaagatctg ccgagcaaca acggggatgt cctgctcgta acgcgtgccc
     1321 aggtccttca ccacggccga ttcgttgttc ggctgcaggc cctcctgcaa catgccggca
     1381 atcgacagcg acatgctgcg cagcgtagcg agatgggcga tcaacctgcc cagcgcggcc
     1441 gcggtggcct tgtccggatt ggcgccggcc atgcgcacca tttcccgcag catctggaag
     1501 aacgccagga aacggtcagg ggcgctgcgc tcgaacgaca actcgctggt gacctgcttc
     1561 cagccctcgc cctccttgcc cagcagcagc cagtccggaa cgaacacgtc ggtcaggaag
     1621 atctcgttga aatggtgctg gccaagcagg ttcaggaccg gacggatctc aatgcccggc
     1681 gttttcaggt ccagcaggaa ctgcgtggcg ccggcatggc ggtccacctc ctcctgtccc
     1741 tgttgggcca tcttgcagaa caggatggcg tagtcggccc gatgcgcgtt ggacgtccac
     1801 agcttgctgc cgttgatgcg atagccaccg tccaccttaa ccgcgcgggt gcgcgtgccg
     1861 gcgagatcgg agccggaatt cggctcgctc atgccaatgc agaaggtgag ttctcccgcg
     1921 gcgatctgcg gcaggaagaa ctgcttctgc gcctcggtgc cgaaacgcag gatattcggg
     1981 ccgctctgcc ggtcgccggt gtagtgcgcg gagagcggtg cgccggccac cagcatttcc
     2041 tcgatcacta catagcgctc gaacgcgctg cgctcatggc cgccgtattg cttcggccag
     2101 gtcatgccga tccagccgcg ccggcccatg gcgcgcgaga agtcacggct gaactcgcct
     2161 cgagacaatc ccatcaggtg ttcgtgttcc cggatgaagg cgcgcacctc ggtgcgcaat
     2221 gccagcgcct ccggcggcag ttccatcgga tcgaaacgga acatcgggac ggccatgcga
     2281 cgttgctccc ggccttattc ttgctgggcc agatcatagg ggcgcttgcc gccgcctgtc
     2341 atgccccggg gccgggacag cggattggac aatcgcccgg gctgtggcac gctctctgga
     2401 aagtccgagg aatgctcaat ggaaaccaat gccgccgacg tggattttct gtccggtgtc
     2461 cgcgtggtcg atttcaccca gttcgaggcc ggtccgtcct gcaccgaagc cctggcctgg
     2521 ctcggcgccg acgtggtgaa gatcgagaat ccaaggatgg gcgatcccgg ccgccgcctg
     2581 cgcccgggcg aaccggacaa cgatccgtac tatttccacg tattcaacgc caacaagaag
     2641 tcgatcaccg tcaacctgaa atcgccacgc ggcctgcagc tcgtgcagga catgctgcgc
     2701 aaggccgatg tgtgcgtgga gaatttcgcg cccggcacca tcgaacgcct cgggctcggt
     2761 tatgacgcgg tgcgggcgct gaatcccgga atcatctatg cttccgtgaa gggcttcggc
     2821 gagggcagcc cgtacgagaa gaacctggcc ttcgacatga ttgcccaagc ctgcggcggc
     2881 accttcagcg tgaccggcga ggcggacggc ccaccgacgc gtcccggcat gtccctcggc
     2941 gataccggca ccggcatgct gatggcgatc accgtgctgg gggccttgca taagcgtgcc
     3001 gcaaccggca agggctgccg gctgcaggtc gcgatgcagg acgcgatgct gcactacatg
     3061 cgcatcaact tcgccacgca ggcactgacc ggcaggccgg cgcagcgcgg cggcagcaag
     3121 gtcgtcggca tcaataacgc gccgatgggc ctgtatccgt gcaagccagg tggcccgaac
     3181 gactatctgt acatcatgac cagccgcgcc aatccggagc actgggaccg gctgttgaaa
     3241 ctgatcgggc gcgaggacct gatcggcgat ccgcgctacg ccacaccggc gttgcgcgtg
     3301 cagcacgaag ccgaggtcga cgagatcatc gccgcctgga cgcggcagca caccaagcat
     3361 gaagcgatgg atgcaatcgg cagcgtcggc gttccggcgg gcgcggtgct ggacacaatg
     3421 gagctgcaga acgacgaaag cttcgcgcgc cgcggcatca tgcagaccat ccatcatccg
     3481 gcgcatggcg acttcaagat gccggcctgg cccgtgcgca tcgacggtaa gccgccgcgc
     3541 gccgtcacat caccggtgct ggggcagcac acgggtgagg tgctgggcgc gtggctcggg
     3601 atgagcgcgg atgacgtggc gaagttgcgc gacgagggcg cggtatagct tcccggagtc
     3661 atgcccgtgc ttggcacggg catccacgtc tgggtggacg caacagcagg aagtcgtggg
     3721 tggccgggcc aagcccggcc atgacaccgg gggccgtgct atcccacgcc caatgccgtg
     3781 cgcagatcct gcaccacctt gtcggcagtc cgcttgcgcc agcgcacgcg gccgccgatc
     3841 ggcgtgttct cgatttccgg cagcaccttc agcgcgacga acaccggacc cggctgacgc
     3901 agaatgcccg gcagctcccg ccgcagcgct tccagctcgg cgaatgccag agcgcgcgga
     3961 taacccgcac cgcgtgcgat cgcggtgtag tccacgttct tcgcattcgg caccggctgt
     4021 ccgccggtgg tggcatagca ctcgttgtcc agcatgaagt ggtagaagtt ggccggcgcc
     4081 tgctctgccg ccgtcgccag catgcccagg ctcatcagca cgtcgccttc ggagtcgaac
     4141 agcaccacct tgcggtttgg ctgcgccagg gcgaccccaa gcccgaagcc cgcatgtccc
     4201 cccattgccg gatcgcccat cggcaaatcg aaggcggttt cactcatgtt gacccagtgc
     4261 cggccgccgc ggccgggaat cacgatcgca tcgccgcgat gttcggcgaa caggttcagg
     4321 acatccgtcg tatgcatcat cgccgtgctc ccggttagcg gttgaagccg tggtattcat
     4381 cgccaaccag cacgacgacc ggcttgcgcg tgcgctccgc ctcctggaag gcgatggaaa
     4441 tgcggtccgc atcggcgtcg ttttccacca ggtagtagga caggttgaac gccatcagga
     4501 atcgctcggt gtaggtggcc gcggtgtcgc tgttcacgcc gtgccgcgtg tagccgcgat
     4561 agccgaccat cagcaccacc ggcacctgca agcccatcag ccagccgcgg atggaatcgc
     4621 cggattccat catgccggtg ttctggatca ggatcatcgg cttgcgcccg ccggcgtata
     4681 gcccggcagc aatcgacgcg gcgtggccct cgcgcgtgac gccgaccagg cgcaggccgg
     4741 gatccgcctg catcagcagg aacagccagt tggtctcgct gtccggcagc cagaccacgt
     4801 cggtaacgcc gttgcgcttc atctgctgca tcaccgtttc cgggctgagc gcagcggcgg
     4861 ctgtgtcggc catctccatc cccctcgatt gcgttgcggc gattgtgtac ggcgcggagt
     4921 caaacgcaag gatcagcggg ccagcggcag cttggtgccg gccacggcgc gttccgcgag
     4981 aacagcgcgg cggaagcgga acagcttggc gggacggccg ccggtttccg tggccacggc
     5041 accggtctcc tcgaccagct cctgctgttc catcaggcgc cggaagttct gcttgtgcag
     5101 gcggcgtccc gccagcgctt cgaccgcgcg ctgcaactgg cccagcgtga aggccggcgg
     5161 catgagctcg aacaccacgg ggcggtattt gatcttgccg cgcagccgcg cgatgccggt
     5221 ggccagtata cgccggtggt cgtgcgccat agcctggccg agtggagtgg tggcgccgga
     5281 ttc
//
LOCUS       DASSZH010000518         3207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_813097, whole genome shotgun sequence.
ACCESSION   DASSZH010000518 DASSZH010000000
VERSION     DASSZH010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813097"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VFN42_11315"
     CDS             complement(<1..500)
                     /locus_tag="VFN42_11315"
                     /inference="COORDINATES: protein motif:HMM:NF033779.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HET8997246.1"
                     /translation="MDVTGGFPIDLVLFGMVAAFLILRLRSILGRRTGFEGRPQQPPH
                     PTGGAMPAGPVIDAKAEPVTPPAQRPVPDPASPLGQALSRIQSADRSFDPARFLDGAD
                     KAFRIIVTAFAAGDRVTLRNLLSDDTYKAFEQAIAAREAAEQTQVSEIRGMEQVAVEE
                     ANVHGTV"
     gene            612..1166
                     /locus_tag="VFN42_11320"
     CDS             612..1166
                     /locus_tag="VFN42_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET8997247.1"
                     /translation="MILLRHGQSEFNLHFSATRRDPGIIDPRLTELGHAQAAEAAQQL
                     ARERVSRIIVSPYTRALQTAAPLARALGVPVMITPLVRERYAFACDIGTPRTELALGW
                     PEHDFSGVEEVWWPPIEESADSVRDRAALFRAEMAALPDWADTVVVSHWGFILSLTGQ
                     SVMNGQWLRCDPTAPPPQEIVWRA"
     gene            1266..1793
                     /gene="secB"
                     /locus_tag="VFN42_11325"
     CDS             1266..1793
                     /gene="secB"
                     /locus_tag="VFN42_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HET8997248.1"
                     /translation="MSETTPAPQPGGQPGQPPLVVNIQYVKDLSFEVPGAPQIFTQLK
                     AQPQVNINLDVQARRVQEGQSVFEVAIMIRAEAHEAAAQGGNGQASASPTAVFVAELT
                     YAGVFTLSGLPDNAVEPVLLVECPRILFPFARNILADVTRDGGFPPVLLQPIDFVALW
                     QSRRQQQQAPAGATA"
     gene            1941..2123
                     /locus_tag="VFN42_11330"
     CDS             1941..2123
                     /locus_tag="VFN42_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997249.1"
                     /translation="MPDRDPLVTDLLEMALRARKHAEAFAHHPAGQSLLKLVEELEER
                     ARQAMLEKKPEPELDR"
     gene            complement(2313..2723)
                     /locus_tag="VFN42_11335"
     CDS             complement(2313..2723)
                     /locus_tag="VFN42_11335"
                     /inference="COORDINATES: protein motif:HMM:NF023184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HET8997250.1"
                     /translation="MNREVMQASAEANSVRRNREFRTIAAMLRMYCRAHHAPPAAPLC
                     PACRELHDYAQRRLDRCVFGEAKPTCANCTVHCYKASMREQIRQVMVWAGPRMLWRHP
                     VLAIRHLMIDGRRPAPTLTTRKDTTRQSRRAPAE"
     gene            complement(2720..3022)
                     /locus_tag="VFN42_11340"
     CDS             complement(2720..3022)
                     /locus_tag="VFN42_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997251.1"
                     /translation="MLGEILQQLTDPAAAEATLALLSEPVLHDRITAAAAAEACPIGT
                     LVAGKIRHLLDHGDEALWLDLLAAMSASPQPGAAAVTRILALAFPDPVRVRITHRA"
BASE COUNT          493 a         1078 c         1117 g          519 t
ORIGIN      
        1 accgtaccgt gcacgttcgc ttcctcgaca gccacctgct ccatgccgcg gatctcgctc
       61 acctgggtct gctcggcggc ctcacgcgcg gcgatcgcct gttcgaacgc cttgtaggta
      121 tcgtccgaca gcaggttccg cagcgtcacc cggtcgccgg ccgcgaaggc ggtgacgatg
      181 atgcggaacg ccttgtccgc cccgtccagg aaacgcgcgg gatcgaagct gcggtccgcg
      241 ctctggatgc ggctcagcgc ttggccgagc gggctggcgg gatcggggac ggggcgctgg
      301 gccggggggg tgacaggctc cgccttggca tcgatcaccg gcccggccgg catggcgccg
      361 ccggtgggat gcggcggctg ctgcggccgg ccctcgaagc cggtgcgccg gcccaggatg
      421 ctgcgcaggc gcaggatcag gaaggctgcc accatgccga acagcaccag gtcaatcgga
      481 aagccgccag tcacgtccat tgccacaact ccttgtgcgg tacagatagg cgccgcaccc
      541 cgcggtgaca aaccattccc gaggtttccg cggggttgcc ggcggaaaat ccgccctgta
      601 tgcgggttgg catgatcctg ctgcgccatg ggcagagcga attcaacctg catttctccg
      661 ccacacggcg cgatccgggc atcatcgatc ctcggctgac cgaactcggc catgcccagg
      721 cggccgaagc ggcgcaacaa ttggcgcggg agagggtctc gcggatcatc gtctcgccct
      781 acacgcgggc gctgcagacc gctgcgccac tggcccgtgc gttgggcgtc ccggtgatga
      841 tcacgcccct ggtgcgggaa cgttatgcct ttgcctgcga tatcggcacc ccgcggaccg
      901 agttggcgct gggctggccg gagcacgatt tctccggcgt ggaggaggtg tggtggccgc
      961 cgatcgagga gagcgccgat tcggtgcgcg accgcgcggc gctgttccgg gcggaaatgg
     1021 ccgctttgcc cgattgggcc gataccgtgg tggtcagcca ctgggggttc atactgtcgc
     1081 tgaccggaca gagcgtgatg aacggccagt ggctgcgttg cgacccgacc gcgccgccgc
     1141 cgcaggaaat cgtctggcgg gcataatttc gggcgcaaac gcgttggcgg ccccgtgaca
     1201 tccgtgctac cgcgcccgcg ggaatgcgcc ggccccggcg ctgcatcgcg agtacggaga
     1261 gattcatgtc cgagacgaca cccgcccctc agcccggtgg ccagccgggc cagccgccgc
     1321 tggtggtcaa catccagtac gtcaaggacc tctccttcga ggttcccggc gccccgcaga
     1381 tcttcaccca gctcaaggcg cagccgcagg tcaacatcaa cctggatgtg caggcccgcc
     1441 gcgtgcagga ggggcagagt gtcttcgaag tggcgatcat gatccgcgcc gaggcgcatg
     1501 aagccgcggc gcagggtggc aacggccagg cgagcgcgtc gcccaccgcg gtgttcgtgg
     1561 ccgagctgac ctatgctggt gtgttcacgc tgagcgggct gccggacaat gcggtggagc
     1621 cggtgctgct ggtcgagtgc ccgcgcatcc tgttcccctt cgcgcgcaac atcctggccg
     1681 atgtgacccg cgacggcggc ttcccgccgg tgctgttgca gccgatcgac ttcgtggcgt
     1741 tgtggcagtc gcgccgccag cagcagcaag ccccggccgg cgccaccgcc tgagcagtct
     1801 cacggcgtca taccccgccg ccggtgtggc ggggtatgac gactccgccc ggctgtcgcc
     1861 tggctgctac gtacaaaacc gaatggttgc cgacgcgcct gcggcgtacg tgaagctttc
     1921 gaaacccgaa agcttgcgcc gtgcccgacc gcgatccgct ggtgaccgat cttctggaaa
     1981 tggcgctgcg tgcgcgcaag catgctgagg cgtttgccca ccatcccgcc gggcaaagcc
     2041 tgctgaaact cgtcgaagaa ctcgaggaac gggcgcgaca ggcgatgttg gagaagaagc
     2101 cggaacctga gttggacagg taatcctgta ccagccggcc gatgtgtcat ggccgcgctt
     2161 ggcgcggcca cccacggcgt ctttctgcgg cgcctgacaa aaacgtgggt gcccgtgcca
     2221 ggcacgggca tgacacatcg gccggcatca cgggcgcctg ggatcagcct gctgtcgctt
     2281 tgagacgcga ctgccgcgct gccggcggca gctcactctg ctggggcgcg ccgggactgc
     2341 cgtgtcgtgt ccttgcgcgt ggtgagcgta ggcgccggcc gcctgccatc gatcatcaga
     2401 tgccggatcg cgagcaccgg atgccgccac agcatgcgcg gacccgccca gaccatcacc
     2461 tggcgaatct gctcgcgcat gctggccttg tagcagtgca cggtgcagtt ggcgcaggtt
     2521 ggcttggcct ccccgaacac acagcggtcc agccgccgct gcgcataatc gtgcagctcg
     2581 cggcaggcgg ggcagagcgg cgccgcgggc ggagcatgat gcgcccgaca gtacatgcgc
     2641 agcatcgcgg cgatggtgcg gaattcgcgg tttcgccgga ccgaattcgc ctcggctgat
     2701 gcctgcatga cttcgcggtt catgcccgat gcgtgatgcg gacgcgcacc gggtcgggaa
     2761 aggccagcgc caggatacgc gtgaccgcgg cggcgcctgg ctgcggcgag gctgacatgg
     2821 cggcgagcag atccagccac agcgcctcgt cgccgtgatc aagcaggtgg cggatcttgc
     2881 cggcgacgag cgtgccgatc ggacacgctt ccgccgccgc tgcggcggta atgcggtcgt
     2941 gcagcacggg ttcgctcagc agcgccagtg ttgcctccgc tgccgccggg tcggtgagct
     3001 gttgcaggat ctcgcccaac atcgcgttgt ccttactgta ccagcggcga cgccgccgcc
     3061 ggcatcgcaa acccggtgat gcaggcctgg ccggcgagca gcgtctggta ttgcgtgacc
     3121 gcgcgccgcc aggcgctttc ctcgagataa gccgcgatgc gctcgcggac gacggcaaag
     3181 ggcagcgcct cggatgcgac tttccgc
//
LOCUS       DASSZH010000519         5729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_523421, whole genome shotgun sequence.
ACCESSION   DASSZH010000519 DASSZH010000000
VERSION     DASSZH010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5729
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523421"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VFN42_11345"
     CDS             complement(<1..610)
                     /locus_tag="VFN42_11345"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET8997252.1"
                     /translation="MAQDIELVLDAHAEIGESPTWSVAEQALYWIDVKAPALHRFNPA
                     NGATRTWPLPAEIGCFALYRDAPAALLALRTGLHRLDLFSDALTRLADPPYDPALHRF
                     NEGACDGAGRFWLGTMFDPQVEKSAPEPGPLFRYTEAEGLVAQPDVSLTPNGLAWSPD
                     GSVLYFAHSKQRRIYRFAFDVAAGRIGSGDTFAEIPAELGVPD"
     gene            785..1339
                     /locus_tag="VFN42_11350"
     CDS             785..1339
                     /locus_tag="VFN42_11350"
                     /inference="COORDINATES: protein motif:HMM:NF022101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HET8997253.1"
                     /translation="MSQQPMRDRNRPSDEATAPQLTLAQKQGEAFGRALQAMSEMDSH
                     GRAQRAGDYLVGYEVEEAEGMYVPDGNGLLWSEPQQENAHLEVVVRDADDGRFVPGLK
                     VHARLMDAGGKEVGAHELPFLWHPWLFHYGRNLQVPKDGTYSLHIHIDPPQFPRHDKV
                     NGKRYASPVDVHFDQVQVETGQKR"
     gene            1385..2626
                     /locus_tag="VFN42_11355"
     CDS             1385..2626
                     /locus_tag="VFN42_11355"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8997254.1"
                     /translation="MTGHPAPRPSARSQRGLDWLNFFIADVETAFGPFVAVYLASYGW
                     SQGTIGSVLTINTAVALATQAPAGALVDWIHVKRAVIAGCLLLIAGGSLLIALFPTYF
                     PVAAGELLHGITGGAVRTALAAIGLGLVGQRAYHVRVGRNHRYDALGNAATAAGMGAL
                     GYLVSPAAPFYAAAGLCLPAFLTLSVIRGQEIDYARARAAEGRREPKAARWRDIAGNR
                     PLLIFAGCLFLFQFADASMLPLAGERLAADHKNESEIITAALVAVPQLVTALIAVYVA
                     RKADEWGRKHLLVIAFAALLARAALFAFAFSPWYLVGVQVFGGLTAAVIGILTPLIVA
                     DLTTRSGRYNFALGSVSMIASVGATISTTAIGFLTQELGYTTGFIALAAVAALGLAAA
                     WWWMPETVESTRGYQPAETAG"
     gene            complement(2599..3519)
                     /locus_tag="VFN42_11360"
     CDS             complement(2599..3519)
                     /locus_tag="VFN42_11360"
                     /inference="COORDINATES: protein motif:HMM:NF025694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate cyclase domain-containing protein"
                     /protein_id="HET8997255.1"
                     /translation="MRRTVARIEALLHTEVGRNATPLFSASRGGLYGAATALAALPAP
                     RVGVLTGFFVPQADPPAAETDGPAGAALLLAGLHAAGLQCRLLTDTPCRSACLAALAG
                     AGLSSDLLDCVDPAASLADAITAWRQAGIDRVVAIERCGRTAAGPPRNMRGLDMSAFT
                     APLDDLFLAGPWDTIAIGDGGNEIGLGALPQGLIAQHVEHGDSIACVTPASHLIMAGV
                     SHWGVYGLIAALALLRADWRAALLACLDEALDRRILETLVRDGPAVDGVSLRRTATID
                     TLPLPVHAAKLQAIRAVVTGRAQPAVSAGW"
     gene            complement(3533..3823)
                     /locus_tag="VFN42_11365"
     CDS             complement(3533..3823)
                     /locus_tag="VFN42_11365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HET8997256.1"
                     /translation="MPVRLVIAITAAPGKGAELAQAMRGRCAEVMQEPGCQQFEVFQS
                     VLDPDRLTLLELWADQAALDVHAQRNATRPPLPDGLRLGAGEREDYHYNRTR"
     gene            complement(3905..4705)
                     /locus_tag="VFN42_11370"
     CDS             complement(3905..4705)
                     /locus_tag="VFN42_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HET8997257.1"
                     /translation="MDGMMSGGGNLGRTERLRIRSGGFTGATAGLAPGNVQANLVILP
                     RDLAHDFLRFAQANPKPCPVLAVSEAGDPRFPTLGADLDIRTDLPRYRVWRDGELASE
                     PTDVRDVWRDDLVSFAIGCSFSFEEALIEDGIEIRHIACGVNVPMYRTNIQCVPAGVF
                     HGPMVVSMRPLKPADAIRAVQITSRFPSVHGAPVHIGLPQQIGISDIGKPDYGDAVPV
                     GEGELPVFWACGVTPQAVIAQARPAFCITHAPGSMLITDLRNARLAAL"
     gene            complement(4766..5590)
                     /locus_tag="VFN42_11375"
     CDS             complement(4766..5590)
                     /locus_tag="VFN42_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997258.1"
                     /translation="MTLTFLLLVLLRAAVAIVVVVGASALAEAAGPFWGGLIVSLPIS
                     AGPAYVMLGLQHDAAFIAAGALASLAANAVSLLYMFIIIVLAPRLRWPLVLAIGLAVW
                     FAVAWVVRQVHWDLTGVLLLNLAMLGSLLWLSRSARRIDTVRAKPTPRRWFELPMRAL
                     MVGTLTASVVTASQWLGPSLTGMAAVFPIATTGLALVTLPRLGGRATAALFASTLVAM
                     PGFAIAVLVLHLTAQPFGVWWGMVTALGAQLVFAALLLLGHRVPAPRAAAEPEVSA"
BASE COUNT          944 a         1970 c         1978 g          837 t
ORIGIN      
        1 cgtccggcac gcccagttcc gccgggatct cggcgaaagt atcgccgctg ccgatgcggc
       61 cggcagccac atcgaaggcg aaacggtaaa tccgccgctg cttgctgtgg gcgaaataca
      121 gcacgctccc atccggactc caggccagac cgttcggcgt caggctgaca tcaggctgcg
      181 ccaccaggcc ctcggcttcg gtgtagcgga acaagggtcc tggttccggg gcgctcttct
      241 ccacctgcgg gtcgaacatg gtaccgagcc agaaccggcc cgcaccgtcg cacgcgcctt
      301 cgttgaagcg atgcagcgcg ggatcgtacg gtggatccgc cagccgcgtc agcgcgtcgc
      361 tgaacaggtc caggcgatgc agcccggtac gcaatgccag cagcgcggcc ggagcgtcgc
      421 gatacagtgc gaagcagcca atctccgccg gcaagggcca ggtccgcgtc gcgccgttcg
      481 ccggattgaa ccgatgcaga gccggcgctt tgacatcgat ccagtacaac gcctgttcgg
      541 cgaccgacca ggtgggcgac tcgccgattt cggcatgtgc atccagcacc agttcaatgt
      601 cctgcgccat cgcggccttc tccctgactg ctgcgatccg gaacgcccgg gcggcagcgc
      661 ggctgcccgg gcgcgatcaa gtccgccgga gtccaggcca ggatgcgcgc atcctccggg
      721 cggtcgccgc gttgtcatcg caacacccgc caccggcaga tttcccatcc tcaggaggac
      781 cgtgatgagc cagcaaccga tgcgcgatcg caatcgcccc agcgacgagg ccaccgcgcc
      841 gcaactgacg ctggcgcaga agcaaggtga ggcgttcgga cgcgcgctgc aggcgatgag
      901 cgagatggac tcgcacggcc gcgcgcagcg ggccggcgac tacctggttg ggtatgaggt
      961 ggaggaggcg gagggcatgt acgtgcccga cggcaacggc ctgctctgga gcgagccgca
     1021 gcaggagaac gcgcacctgg aggttgtggt gcgcgacgcc gacgacggcc gcttcgttcc
     1081 gggattgaag gtgcacgcgc ggctgatgga tgcgggcggc aaggaggtgg gtgcgcatga
     1141 gctgccgttc ctctggcatc cctggctgtt ccattacggc cgcaatcttc aggtgccgaa
     1201 ggacggcacc tacagcctgc acattcatat cgacccgccg cagttcccgc gccacgacaa
     1261 ggtgaacggc aagcgctacg catcaccggt ggatgtccat ttcgaccaag tccaggtgga
     1321 gaccgggcag aagcgctgac gcgccgtcgc aaccggcagc aaggcatcga aaacaggacg
     1381 ggccgtgaca ggacaccccg cgccacggcc ctcggcccgg agccagcgcg ggctggactg
     1441 gctgaatttc ttcatcgccg acgttgagac cgcgtttggc cccttcgtcg ccgtctacct
     1501 ggcatcctat ggctggagcc agggcaccat cggttcggtg ctgacgatca acaccgccgt
     1561 cgccctggcg acgcaagccc ctgccggcgc cctggtggat tggattcacg tgaagcgcgc
     1621 ggtcatcgcc ggatgcctgc tgctgattgc cggcggatcc ctgctgatcg cgctgtttcc
     1681 cacgtatttt ccggtcgcgg cgggggaact gctgcacggc atcaccggcg gcgcggtgcg
     1741 cactgccctg gcggcgatcg gcctgggtct cgtgggccag cgcgcctacc atgtgcgggt
     1801 cgggcgcaac caccgctatg acgccttggg caatgcggcg accgcagccg ggatgggggc
     1861 gcttggctac ctcgtctcac ccgccgcgcc cttctatgcc gccgccgggc tgtgcctgcc
     1921 cgcctttctg accctcagtg tcatccgcgg tcaggagatc gactacgccc gcgcgcgtgc
     1981 ggcggagggc cgccgggaac caaaggcggc acgctggcgc gacatcgccg gcaaccggcc
     2041 gctgctgatc ttcgccggct gcctgttcct gttccagttc gccgacgcct cgatgctgcc
     2101 gctggccggt gaacgcctgg ccgcggacca taagaatgag tcggagatca tcaccgcggc
     2161 gctggtggcg gtgccgcagc tcgtgacggc gctgatcgcg gtgtacgtcg cccgcaaagc
     2221 ggatgaatgg ggccgcaaac acctgctggt gatcgcgttt gccgccctgc tggcacgcgc
     2281 cgcgctgttc gccttcgcct tcagcccctg gtatctggtg ggcgtgcaag tgtttggcgg
     2341 gctgacggcg gcggtgatcg gcatcctgac gccgctgatc gtcgccgacc tcaccacccg
     2401 cagcggccgc tataacttcg cgctcggctc ggtcagcatg atcgccagcg tgggtgccac
     2461 gatcagcaca acggcgatcg gctttctcac ccaggaactc ggctacacta cgggctttat
     2521 cgcgctcgcg gcggtcgcgg cgctgggtct ggcagccgca tggtggtgga tgcccgagac
     2581 ggtggaatcc acccgcggct accagccggc cgagactgcc ggctgagctc tgcccgtcac
     2641 caccgcgcgg atggcctgca gcttcgccgc atgcaccggc agcggtagcg tgtcgatggt
     2701 cgcggttcgc cgcagcgaga cgccgtccac cgcaggcccg tcgcgcacca gggtttccag
     2761 gatgcggcga tcgagggctt catccaggca ggccaacagc gccgcacgcc aatccgcgcg
     2821 cagcagcgcc aaggccgcga tcaggccgta cacgccccag tgcgacacgc ccgccatgat
     2881 caggtgcgat gccggggtga cacaggcaat gctgtcgcca tgctccacgt gctgcgcaat
     2941 gaggccctgc ggcagcgcgc ccagaccgat ctcgttgccg ccgtcgccaa tcgcgatggt
     3001 atcccacggg ccggccagga acagatcgtc caacggggcg gtgaaggcgc tcatgtccag
     3061 gccgcgcatg ttgcgcggcg ggcccgcggc ggtgcggccg cagcgctcga tcgccaccac
     3121 ccggtcgatg ccggcctggc gccacgcggt gatcgcatcg gccagggagg cggcggggtc
     3181 cacgcaatcc agcagatcag acgaaagacc agccccggcc agggccgcca ggcaggcaga
     3241 gcggcagggg gtgtcggtca gcagccggca ttgcaggccg gcggcatgca gccccgcgag
     3301 cagcagcgct gcgccggcgg gtccgtcggt ctccgccgcg ggcggatccg cctggggaac
     3361 gaagaacccg gtcagcacgc cgacacgcgg cgccggcagc gccgccagtg cggttgcggc
     3421 accatacaga ccgccgcggc tggcgctgaa cagcggcgtg gcgttgcgcc cgacttcggt
     3481 gtggagcagc gcttcgatcc gcgcgaccgt gcggcgcatt gatccgaagc ggtcagcggg
     3541 tgcggttata gtggtagtcc tcgcgttcac ccgcgcccag gcgcaggcca tccggcagcg
     3601 gcggccgcgt agcgttgcgt tgcgcgtgga cgtcgagcgc cgcctggtcc gcccataatt
     3661 ccagcaaggt cagccggtcc ggatcgagca cgctctggaa cacctcgaac tgctggcagc
     3721 caggttcctg catcacctcg gcacagcgtc cgcgcattgc ctgtgccagt tctgccccct
     3781 tgccgggcgc ggcggtgatg gcgatgacaa gccggacggg catggcgatt ggttcccctg
     3841 cgcgaatgat gccgcgcagt agcggcgctg ccgcgccgcg cgtcaagctt gccgcccgtg
     3901 cctgctacag cgcggcgagg cgggcgttgc gcagatcggt gatcagcatg cttcccggtg
     3961 cgtgggtgat gcagaacgcc ggacgcgcct gcgcgatcac cgcttgcggg gtgacgccac
     4021 aagcccagaa caccggcagt tcgccttcgc cgactggtac cgcgtcgccg taatccggct
     4081 tgccgatgtc ggaaattcca atctgctgcg gcaggccgat atgcaccggg gcgccatgca
     4141 ccgatgggaa gcgcgaggtg atctgcaccg cccggatcgc atccgcgggc ttcagcgggc
     4201 gcatcgaaac gaccatcggc ccatgaaaca cgccggccgg cacgcattgg atgttggtgc
     4261 gatacatcgg cacgttcacg ccgcaggcga tgtgacggat ctcgatgcca tcctcgatca
     4321 gcgcctcctc gaaggagaag gagcagccga tggcgaagct caccaggtcg tcccgccaca
     4381 catcgcgcac gtcggtgggt tcgctggcca gttcgccgtc acgccagacc cgatagcgcg
     4441 gcaggtcggt gcggatatcc aggtcggccc ccagggtcgg aaagcgtgga tcgccggctt
     4501 cagacaccgc caggaccggg cagggtttcg ggttggcctg cgcgaagcgg aggaagtcat
     4561 gtgccaggtc gcgcggcagg atcaccaggt tggcctgcac gttgccaggg gcgagacctg
     4621 cggtcgcgcc ggtgaacccg ccggaccgga tgcgcagccg ttcggtccgc ccgagattgc
     4681 cgccgccgct catcatgccg tccatcggcg cctcctgttc tgggtgcgga tcacggatgg
     4741 agcgttctcc agccggccag acaggtcaag ccgatacttc cggttctgcc gccgcccgcg
     4801 gggcgggcac ccggtgtccc agcaacagca atgccgcaaa gaccaactgc gcgccgagcg
     4861 cggtcaccat accccaccac acgccgaagg gctgcgcggt caggtgcagc accagcacgg
     4921 cgatggcgaa gcccggcatc gcgaccagcg tgctggcgaa cagcgcggct gtggcgcgac
     4981 cgccgaggcg cggcagcgtc accagcgcca gaccggtggt ggcgatggga aacactgccg
     5041 ccatgccggt caaggatggc ccgagccatt ggctggcggt gaccacgctc gcggttagcg
     5101 tgccgaccat cagggcgcgc atcggcaatt cgaaccagcg ccggggcgtc ggcttggcgc
     5161 gcaccgtatc gatccggcgg gcggagcggg agagccacag caggctgcct aacatcgcca
     5221 ggttcagcag caggacaccc gtcagatccc agtgcacctg ccgcaccacc caggccacgg
     5281 cgaaccacac ggccagcccg atcgccagca ccagcggcca gcgcaggcgc ggcgccagca
     5341 cgatgatgat gaacatgtac agcagcgaga ccgcattggc ggccaggctg gccagcgcgc
     5401 cggcggcgat gaacgcggcg tcgtgctgca ggccgagcat cacgtaggcc gggccggcgg
     5461 agatcggcag gctgacgatc agcccgcccc agaacggccc ggcggcttcc gccagcgcgg
     5521 aggcgccgac cacgacgacg atcgccacgg cggcgcggag gaggacgagg agcaggaagg
     5581 tgagggtcat gtcagcagct gggtcacggg gctctgttca tcagccggcc tcaagatagt
     5641 cccgcgcgga caggatccga attcctcgcc aggattcagg tccagcaaat cgcgatcgct
     5701 ggagatgatg caatccgcgg agaccgagg
//
LOCUS       DASSZH010000520         3535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_642978, whole genome shotgun sequence.
ACCESSION   DASSZH010000520 DASSZH010000000
VERSION     DASSZH010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642978"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2516)
                     /gene="uvrA"
                     /locus_tag="VFN42_11380"
     CDS             complement(<1..2516)
                     /gene="uvrA"
                     /locus_tag="VFN42_11380"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET8997259.1"
                     /translation="MAGSIHVRGAREHNLKNVDVEIPRDSLTVITGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLELMGKPDVDSIDGLSPAISIEQKTTSKNPRSTVGTV
                     TEIHDYMRLLWARAGVPYSPATGLPIEAQTVSQMVDRVMAMPEGTRLLLLAPVVRDRK
                     GEYRKELAELQRRGFTRVKVDGKLYEIGDVPALNRKVKHEIEAVVDRIVVRDGIAPRL
                     ADSFETALGLSDGVVYAENADDSERTVFSSRFACPISGFTIEEIEPRLFSFNSPHGAC
                     PACDGLGVETFFDPELVVPDERKTLAQGAIAPWTGAQSPYYDQTLQSLAKHYKVTTST
                     PWQDLPQRVRDGILHGTGDEPVALTYKDGVRAYTVTKPFEGVLRNLQRRWQETDSVWL
                     REELSRYQAEKPCAACNGARLKPEALSVRVAGRNIAEASEQSIRNALAWFQDVLPQLT
                     PQRQEIARRILREIVDRLQFLVDVGLDYLTLARGSATLSGGESQRIRLASQIGSGLTG
                     VLYVLDEPSIGLHQRDNERLLGTLRRLRDIGNTVLVVEHDEDAIRAADHLIDMGPAAG
                     VNGGRVVAQGTPAEVTANPKSLTGDYLSGRKRIEVPQLRRPVQRNNVLRVVGARGNNL
                     KQVTAEFPLGTFTCVTGVSGGGKSTLVVDTLYKAAARRLMGSGLVPAPHDRIENLELL
                     DKIIDIDQSPIGRTPRSNPATYTDLFAPIRDWFAELPEARARGYKPGRFSFNVKGGRC
                     EACQGDGVLKIEMHFLPDVFVTCDTCKGRRYNRETLEIKFRDKSIAEVLAMTVDEAVP
                     YFSAQSRIRDRLKILQQVGLGYISLGQQATTLSGGEAQRI"
     gene            2668..3180
                     /gene="ssb"
                     /locus_tag="VFN42_11385"
     CDS             2668..3180
                     /gene="ssb"
                     /locus_tag="VFN42_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010507163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET8997260.1"
                     /translation="MAGSVNKVILVGNLGKDPEVRTTQDGTKIVHLTLATSETWNDRS
                     SGERKERTEWHRVVIFNDRIGDVAERFLRKGRKVYVEGSLQTRKWTDQSGQERYTTEV
                     VIGRFRGELVLIDSQRGEEGGMGEGSGGGGYNRDRAPASRPAGGGGRPSGGPSWEAPK
                     GGDLDDEIPF"
BASE COUNT          555 a         1132 c         1225 g          623 t
ORIGIN      
        1 ttgatccgct gcgcctcccc gcccgagagc gtggtcgcct gctggccgag gctgatgtag
       61 ccgagcccca cttgttgcag gatcttcagc cggtcgcgga tgcgcgactg cgcggagaaa
      121 tacggcaccg cctcatccac cgtcatcgcc agcacctcgg cgatcgactt gtcgcggaac
      181 ttgatctcca gcgtttcgcg gttgtagcgg cggcccttgc aggtgtcgca ggtcacgaac
      241 acgtccggca ggaagtgcat ctcgatcttc agcacgccgt cgccctggca ggcctcgcag
      301 cgcccgccct tcacgttgaa gctaaaccgg cccggcttgt agccgcgcgc acgcgcttcc
      361 ggcagctcgg cgaaccagtc gcggatcggc gcgaacaggt cggtgtaggt ggcggggttg
      421 gagcgtggcg tgcggccgat cggcgactgg tcgatgtcga tgatcttgtc gagcagttcc
      481 agattctcga tgcggtcgtg cggcgccggc accagcccgg agcccatcag ccggcgcgcc
      541 gcggccttgt acaacgtgtc caccaccagc gttgacttgc cgccgccgga cacgccggtc
      601 acgcaggtga aggtgcccag cgggaattcc gcggtcacct gcttcagatt gttgccgcgg
      661 gcgccgacca cgcgcagcac gttgttgcgt tgcaccggcc ggcgcagttg cggcacctcg
      721 atgcgtttgc ggccggacag gtaatcgccc gtcagcgact tcgggttggc ggtgacctcg
      781 gccggcgtgc cctgcgccac cacgcggccg ccattgacgc cggccgcagg gcccatgtcg
      841 atcaggtggt cggcggcgcg gatcgcgtct tcgtcgtgct ccaccaccag cacggtgttg
      901 ccgatgtcgc gcagccgccg cagcgtgccc agcaggcgtt cgttgtcgcg ctggtgcagg
      961 ccgatggagg gttcgtccag cacgtacagc acaccggtca ggccggagcc gatctggctg
     1021 gcgagacgga tgcgctgcga ttcgccaccg gacagcgtgg cggaaccgcg cgccagcgtc
     1081 aggtaatcca gcccgacatc caccaggaac tgcagccggt caacgatttc ccgcaggata
     1141 cgccgggcga tttcctggcg ctgcggcgtc agttgcggca gcacgtcctg gaaccatgcc
     1201 agcgcattgc ggatcgactg ctccgacgcc tcggcgatgt tgcggccggc gacgcgcacc
     1261 gacagcgcct ccggcttcag ccgcgcgccg ttgcaggcgg cgcagggctt ttccgcctgg
     1321 tagcgcgaca gttcctcgcg cagccagacg ctgtcggttt cctgccagcg acgctgcagg
     1381 ttgcgcagca cgccttcgaa cggcttggtg acggtgtagg cgcgcacgcc gtccttgtag
     1441 gtcagcgcta cgggttcatc gccggtgccg tgcaggatgc catcgcgcac gcgttgcggc
     1501 aggtcctgcc acggcgtgct ggtggtgacc ttgtagtgct tcgccaggct ttgcagcgtc
     1561 tggtcgtagt acgggctctg tgcaccggtc cacggcgcga tggcgccctg cgccagggtc
     1621 ttgcgttcgt ccggcaccac caattccgga tcgaagaagg tttccacgcc cagcccgtcg
     1681 caggccgggc aggcgccgtg cggcgagttg aagctgaaca gccgcggctc gatttcctcg
     1741 atggtgaagc cgctgatcgg acaggcgaag cgcgaggaga acaccgtgcg ttcggaatcg
     1801 tcggcgtttt cggcgtacac cacgccatcc gacaggccga gggcggtttc gaagctgtcg
     1861 gcgaggcggg gggcgatgcc gtcgcgcacg acgatacggt ccaccaccgc ttcgatctcg
     1921 tgcttcacct tgcgattcag cgcgggtacg tcgccgattt cgtagagctt gccgtccacc
     1981 tttacccggg tgaagccgcg gcgttgcagt tcggcgagtt ccttgcggta ttcgcccttg
     2041 cggtcgcgca ccaccggcgc gagcagcaac aggcgcgtgc cctccggcat cgccatgacg
     2101 cggtccacca tctgcgacac ggtctgcgcc tcgattggca ggccggtggc gggcgagtac
     2161 ggcacgccgg cgcgcgccca gagcaggcgc atgtagtcgt ggatctccgt caccgtgccg
     2221 acggtggaac ggggattctt gctggtggtc ttctgttcga tggaaatcgc cggcgacagc
     2281 ccgtcgatgc tgtccacgtc gggcttgccc atcagttcca ggaactggcg ggcataggcc
     2341 gacagtgact cgacatagcg gcgctggccc tcggcgtaga tcgtgtcgaa ggcgagcgac
     2401 gatttgccgg agccggacag cccggtgatc acggtgagcg aatcgcgcgg gatttcgaca
     2461 tccacgttct tcagattgtg ctcgcgcgcg ccgcgcacat ggatggagcc ggccattctg
     2521 gcagtcccgc ttgttctcag attgttcgca cgttatatgg cgcagttcgg gccgcttggg
     2581 aaggtccgat taaccattat cggatagcat ggccccagcc cggcccatcc tgtaggcttg
     2641 gctcctggaa tgtgaggaat tcgtgcgatg gcaggcagcg tgaacaaggt gatcctggtg
     2701 ggcaacctgg gcaaggaccc ggaggtgcgc accacgcagg acggcacgaa gatcgtgcac
     2761 cttacgctgg ccaccagcga aacctggaac gaccgcagtt cgggggagcg caaggagcgc
     2821 accgaatggc atcgcgtggt gatcttcaac gaccggatcg gcgacgtggc ggagcggttc
     2881 ctgcgcaagg gccggaaggt ctacgtggaa gggtcgctgc agacccgcaa gtggaccgat
     2941 cagagcgggc aggaacgcta taccaccgag gtggtcatcg gccggttccg cggcgaattg
     3001 gtgctgatcg acagccagcg cggcgaggaa ggcggcatgg gtgagggcag cggtggtggc
     3061 ggctacaacc gcgaccgggc tccggcgtcg cggccggccg gcggcggcgg acggccgagc
     3121 ggcggccctt cgtgggaggc gccgaagggt ggcgacctgg acgacgaaat tccgttttag
     3181 aaatggaacc gcagatgaac gcggatgaac gcggatggtc ttgagcgggc cgtagcccgg
     3241 tcgcgtacgt ccgttcagcc agggccacag taaaatccgt ccgcggaaag ttcgactctc
     3301 gggctggccg actccgtcgt ggcgggcgaa ggcccgccat ccacgacttg cagggggaga
     3361 ctcccaagcc gcctgcagtc atgagtttcc cgaaacaaat cgtgggtggc gggcctgcgc
     3421 ttgccacgac gttgtttgcg cgtcaggtgt cactctctgc gcggcttggt gatactattg
     3481 ccttcggcga aggttcgctc ggctgaccgg gcgtccccgc agcatccgcg ttcat
//
LOCUS       DASSZH010000521         4222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1392967, whole genome shotgun sequence.
ACCESSION   DASSZH010000521 DASSZH010000000
VERSION     DASSZH010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392967"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="VFN42_11390"
     CDS             <1..973
                     /locus_tag="VFN42_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET8997261.1"
                     /translation="AVAWARAAGAVVMGKTVTTEFATRRPGATTNPANPEHTPGGSSS
                     GSAAGVAAGFFPIAYGTQTAGSVIRPAAYCGVVGYKPSFGTINRFGMKLMADSLDTVG
                     VMARSVADCALFAGAVSGRDLGDPVVKPLNAPRIGICRSPSWNQAGAETQGLLDRVAS
                     ALGRAGAALSERELPAEYNTLEQAHPIVMNSESARALGWELAHHRDGISPELLERLEF
                     GLAQPDAVLAEAYAVFARTQRGFPVAMEGLDVLVTPSAPGEAPKGIAWTGDPAFNYLW
                     TSLHVPCVTVPAGEGPNGLPLGIQIVGRLGEDRAVLAWAQWVAAAIG"
     gene            complement(987..1883)
                     /locus_tag="VFN42_11395"
     CDS             complement(987..1883)
                     /locus_tag="VFN42_11395"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HET8997262.1"
                     /translation="MWPIRSALFVPAHRRDWVGKALRVSPGAVVLDIEDSVPPEHKQA
                     AMRHLKDEIAELRAAGVAAFIRINPLSDGTAAEIAAAVTEGCTAIVLPKTASVAEIHR
                     LHDLLSYAEGRAGVAHGSVGILPLPETAEGMYAARDLAAASPRVKGIHGALSGPVSGD
                     FARAFGFRATSDGTEQLYLASKLVLDSRAGGAMYPIAGIFGTPQDDLAAVERLIRRAR
                     DLGYSGVAVMHPTHVPVANAVYRPTPEEVAYFEGLLAAFEAAEKAGQGAVSYHGAMVD
                     YAMLPLAREVLEEARRFGVKAG"
     gene            complement(1944..2357)
                     /locus_tag="VFN42_11400"
     CDS             complement(1944..2357)
                     /locus_tag="VFN42_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997263.1"
                     /translation="MRNHGWLAAAILAVSACLCGPVRAAPPTGGSYVTTIPPQVSNPP
                     PLSPAGEQVVPNDPEFSRECLQMIPALRKNIHMERALTTQTRQWGMVMRIDFTMTATQ
                     MQTIAGRVNRLVFWRRTGDSPSVLIAVGQPLAPLP"
     gene            complement(2371..3531)
                     /locus_tag="VFN42_11405"
     CDS             complement(2371..3531)
                     /locus_tag="VFN42_11405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET8997264.1"
                     /translation="MDFTLTDEQQQIRESIARICAEFGDEYWYRKDLHHEFPHEFARA
                     FAAAGWFGAIFPEEVGGAGLGLTEAAIIMHEVGRLGFNACSTLHMNMFGCQPIVKFGT
                     DEQKRRFLPRIIDGTDRACFGVTEPDAGLDTTHIRTFATRHNDRYVVHGRKVWTSTAQ
                     VANKILLLTRTQKFEDSAKPSAGMTLFYTDLDRSKVEVRLIEKLGRNAVDSNALFIDG
                     LEIPMEDRIGEEGRGFQYLLHGLNSERIIVAAGILGASRYVLDKAVTYARDRVVFGRP
                     IGQNQAIQHPLAECWIEHQAAELMLFKAAALYDSGAECGPQVNAAKYLCAEVFFKSAT
                     RAVRTHGGMGYAKEFQVERYFRESILPLIAPVSQEMVLNYVAERALGLPKSY"
     gene            complement(4036..>4222)
                     /locus_tag="VFN42_11410"
     CDS             complement(4036..>4222)
                     /locus_tag="VFN42_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HET8997265.1"
                     /translation="PIGQIWSAALMLEHLGEAEAGKAIVAAIETLLKESGPKTRDMGG
                     QAGTADVGDAIAQIVAG"
BASE COUNT          648 a         1409 c         1461 g          704 t
ORIGIN      
        1 cgcggtggcc tgggcacggg cggcgggcgc ggtggtgatg ggcaagaccg tcaccacgga
       61 gttcgccacg cgcaggccgg gtgccacaac caatccggcc aatccggagc atacacccgg
      121 cggctcgagc agcgggtcgg cggccggcgt cgcggcgggg ttctttccga tcgcctatgg
      181 cacgcagacc gcgggcagcg tgatccggcc ggcggcttat tgcggggtgg tgggctacaa
      241 gccgagcttc gggacgatca accggttcgg catgaagctt atggcggaca gcctggacac
      301 ggtcggggtg atggcgcggt cggtcgccga ctgcgcgctg ttcgcgggcg cggtgtccgg
      361 ccgggatctg ggcgatccgg tggtgaagcc gctgaacgct ccgcgcattg gcatatgccg
      421 gtcgccgagc tggaaccagg cgggggcgga gacgcaggga ctgctggacc gggtggcctc
      481 ggccctggga cgggccggcg cggcgctaag cgagcgcgaa ctgccggccg aatacaacac
      541 cctggagcag gcacatccga tcgtcatgaa cagcgagagc gcccgtgccc tgggctggga
      601 attggcgcac caccgtgacg ggataagccc ggagctgttg gagcggctgg agttcggcct
      661 ggcccagccg gacgccgtgc tggccgaggc ttacgcggtc ttcgcccgca cccagcgcgg
      721 cttccccgtg gcgatggagg ggttggatgt gctggtgacg ccctccgccc ctggcgaggc
      781 gcccaagggg atcgcatgga ccggcgatcc ggccttcaac tatctttgga ccagcctgca
      841 cgtaccttgt gtgaccgtgc cggccggcga aggcccgaac ggcctgccgc tgggcatcca
      901 gatcgtcggc cggctgggcg aggaccgcgc ggtgctggcc tgggcgcaat gggtggcagc
      961 ggcgatcggg tagggtctcc ccaccctcac ccagccttca ccccgaagcg ccgcgcttcc
     1021 tccagcactt ccctggccag cggcagcatg gcgtagtcca ccattgcccc gtggtagctg
     1081 accgcgccct ggccggcctt ttccgccgct tcgaacgccg ccagcaggcc ttcgaaatag
     1141 gccacctcct ccggggtggg gcgatacacc gcattggcca ccggaacatg cgtcgggtgc
     1201 atcaccgcga cgccgctgta gcccaggtcg cgcgcccgcc ggatcagccg ttccaccgcc
     1261 gcaagatcgt cctgcggcgt gccgaagatg ccggcgatcg ggtacatcgc cccgccggcc
     1321 ctgctgtcca gcaccagctt ggaggcgaga tacaactgct cagtgccgtc gctggtggcg
     1381 cggaaaccga aggcgcgagc gaagtcacca gagaccgggc cgctgagtgc gccatgaatg
     1441 cccttcacgc gcggactggc agcggccagg tcacgcgcgg cgtacatgcc ctcggcggtt
     1501 tccggcagcg gcaggatgcc gacgctgcca tgcgccacgc cggcccggcc ttccgcgtag
     1561 ctgagcaggt cgtgcagccg gtggatctcc gccacgctgg cggtcttcgg cagcacgatg
     1621 gcggtgcagc cctccgtgac ggcggcggcg atttccgccg cggtgccgtc ggacagcggg
     1681 ttgatgcgga tgaacgccgc gacaccggcg gcacgcagct cggcgatctc gtccttcaga
     1741 tggcgcatcg ccgcctgctt gtgctcgggc ggtacggaat cctcgatgtc cagcaccacc
     1801 gcacccggcg acacgcgcag cgccttgcca acccagtcgc ggcgatgcgc cggcacaaac
     1861 agcgcggagc ggatgggcca catcggtctg tctccatctg tgatcgttgc aatcatgcta
     1921 gtcatcgccg gcggccacgc aaactacggc agcggcgcca atggctggcc caccgcgatc
     1981 agcactgacg gtgagtcgcc ggtgcggcgc cagaacacca ggcggttcac ccgcccggca
     2041 atcgtctgca tctgcgttgc cgtcatggtg aagtcgatgc gcatcaccat gccccattgc
     2101 ctggtttgcg tggtcagggc gcgttccatg tggatgttct tgcgcagcgc cgggatcatc
     2161 tgcaggcact cgcggctgaa ttccggatcg ttcggcacga cttgctcgcc ggccggactg
     2221 agcggcggcg ggttgctgac ctgcggcgga atggtggtga cataggaccc gccggtcggc
     2281 ggcgccgcgc gtaccggccc gcacaggcag gctgagactg cgaggatcgc ggcagccagc
     2341 catccgtgat tgcgcacccc gtcgcgccga tcagtatgat ttgggcaggc cgagcgcgcg
     2401 ttcggcgacg tagttcagca ccatttcctg gctgaccgga gcgatcagcg gcaggatgga
     2461 ttcgcggaaa tagcgttcca cctggaactc cttggcgtaa cccatgccgc catgcgtgcg
     2521 cacggcgcgg gtggcggatt tgaagaacac ttccgcgcag agatacttcg ccgcattcac
     2581 ctgcggcccg cactcggcac cgctgtcata cagcgcggca gccttgaaca gcatcagctc
     2641 tgccgcctgg tgctcgatcc agcattccgc cagcgggtgc tggatcgcct ggttctggcc
     2701 gatcggccgg ccgaacacca cgcggtcgcg cgcataggtc accgccttgt ccagcacgta
     2761 gcgcgatgcg cccaggatgc cggcggcgac gatgatgcgc tcggaattca acccgtgcag
     2821 caggtactgg aagccacggc cttcctcgcc gatgcggtcc tccatcggga tctccagccc
     2881 gtcgatgaac agcgcgttgg agtccacggc attgcggccg agcttctcga tcagccgcac
     2941 ctccaccttg ctgcggtcga ggtcggtgta gaacagcgtc atcccggcgc tcggcttggc
     3001 gctgtcctcg aatttctgcg tgcgggtgag cagcaggatc ttgttcgcca cctgcgcggt
     3061 ggaggtccac accttgcgcc catgcaccac gtagcggtcg ttgtgcctgg tggcgaaggt
     3121 gcggatatgc gtggtgtcca gcccggcatc cggctcggtg acaccgaagc aggcgcggtc
     3181 ggtaccgtcg atgatgcggg gcaggaagcg gcgcttctgc tcgtcggtgc cgaacttcac
     3241 gatcggctgg cagccgaaca tgttcatgtg cagggtggag caagcgttga agccgagccg
     3301 gccgacttcg tgcatgatga tggccgcttc ggtcaggccg agaccggcgc cgcccacctc
     3361 ctccgggaag atcgcaccga accagccggc ggcggcaaag gcgcgggcga attcatgcgg
     3421 gaattcgtgg tgcagatcct tgcggtacca atattcgtcg ccgaactcgg cgcagatgcg
     3481 ggcgatgctc tcgcggatct gctgctgttc gtcggtaagg gtaaagtcca tccgccgcct
     3541 ccgtccccgg gcggcagatt gtgggcggcg gcgggagcgg tggcaatgcg gcggtctgtg
     3601 ctgtctcgcg ggcgtctcgt tgtacgttcc gcgggattat gtcccatggc atgagcgggg
     3661 accggcggaa cgccgtctca gcgccatctc attgcccgct atttgtctcg gagtctctgc
     3721 ctgtgggatc agaggccgcg tgcgggaatt gcagacgcgg aatctggtat ccgcactatg
     3781 gcccgcggcc cgggccaaag cggagaggca agagccagtc gaaacccgaa cgtatagaga
     3841 acactcaact gcatcatgtg tcaagcattt tcgtcggcag cacttttgtc gaacgggcgc
     3901 ggggggtcac gccagcctga tcgcaaagcg ctcccaccgc atcgcactgg cagagcgctg
     3961 gtccatggtg cagggccggc catggcgcgc cggtgtgcgg ccccgcggtc tgcagcgccg
     4021 atcccggcgg atcggctaac ccgccacgat ctgcgcgatg gcgtcgccga catcggcggt
     4081 tccggcctgg ccgcccatgt cgcgggtctt cggcccgctc tccttcagca gcgtctcgat
     4141 cgcggcgacg atcgccttgc cggcttccgc ctcgcccaga tgctccagca tcagcgccgc
     4201 cgaccatatc tggccgatcg gg
//
LOCUS       DASSZH010000522         3482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_915323, whole genome shotgun sequence.
ACCESSION   DASSZH010000522 DASSZH010000000
VERSION     DASSZH010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915323"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VFN42_11415"
     CDS             complement(<1..526)
                     /locus_tag="VFN42_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET8997266.1"
                     /translation="MSETAASRLRLARHVGLFAVMASAVAGEYGAGINFVAVQSLSVY
                     PAVQYLVPVAMAVTGLVLVTKVFMFARFSRVMPRAGSAYVWIVRSVDPLLGFIAGFLW
                     WISLTAAMGFIAFAFSTFLGSALTGLGLPGALLTTAPGRTVIGLAAIWGIYALHAAGV
                     RHYGTFVSVLLWLIV"
     gene            complement(714..>3482)
                     /locus_tag="VFN42_11420"
     CDS             complement(714..>3482)
                     /locus_tag="VFN42_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET8997267.1"
                     /translation="YDLAGKLRTELTKVPNIGTTYMVGTGPEQIRVEPDPEKLSLFGV
                     TLQQLIAKVQGANREFVAGSVRDAGVTRTLAAGQTLRGVPDIGMLLLTTRDGRPVYVR
                     DVARVIVGANTSDSRVWDITPGKPGWQREPAVTIALAKRGGANAVVVAQDIVQRVDGL
                     KGRLIPSDVRVQVTRDYGRTANDKANELLFHLALATVSIVVLIALAIGWREAVVTLVV
                     IPTTILLTLFSAKLMGYTINRVSLFALISAIGILVDDAIVVVENIARHWAMRDRRSRL
                     RAAVEAVSEVGNPTIVATLTVVAALLPMLFVSGLMGPYMAPIPANASAAMLFSFFVAV
                     VVAPWLLCKLAPEPGGAARLDEEVEWLPASAPGIEHDMAGHHEGRLGRIYRAVATPIL
                     KTRARAWTFLLLVGFGTIAACALFYTEDVTVKLLPFDNKSELALELNLPEGSSLEDTE
                     RTLFAAARIVEAVPEVRSVQAYAGTAAPFNFNGLVRHYYMRQSPELGDLQINLADKAD
                     RDRSSHAIALDLRNRLKALHLPAGATTQVVEVPPGPPVLATLLAEIYGPDAKTRLAVA
                     QEVKTLFKSVPYIVDVTDSWGHPRPRLRISIDQDELEYFGVEQSDVYDTIQALFGGVP
                     VGYSHRGAERDPIEIVVGLPKRDMSWNEMLAATPVPANTLPGSKTVVELGQVVRAQQE
                     LGSRVLFRRDGHFADMVMGELAGEYEAPIYGMLAVDKLVAQHDWGKLPKPVISLRGQP
                     TDETHPTLLWDGEWEITYVTFRDMGAAFMVAMLGIYILVVAQFRSFKLPLVILTPIPL
                     TLIGILLGHWLFHAPFTATSMIGFIALAGIIVRNSILLVDFVRHGAGQGKTLRAVLLN
                     AGAIRFKPILLTALAAMIGAATILTDPIFQGLAISLLFGLASSTLLTVLVIPAIYVVL
                     RDADRVLT"
BASE COUNT          602 a         1160 c         1186 g          534 t
ORIGIN      
        1 gcacgatcag ccacagcagg accgagacga aggtgccgta gtgccgaacg ccggcggcgt
       61 gcagcgcgta aatgccccag atggcggcga gaccgatgac ggtgcggccg ggtgcggtgg
      121 tgagcagggc gccgggcagg cccaagcccg tcagcgcgct gccgaggaag gtggagaacg
      181 cgaaagcgat gaaccccatg gcggcggtca gcgagatcca ccacaggaag ccggcgatga
      241 agccgagcag cggatcgacg ctgcgcacga tccacacata ggccgacccg gcgcgcggca
      301 tgacgcgcga gaagcgcgcg aacatgaaga ccttggtgac cagcacgagg ccggtaaccg
      361 ccatcgccac cgggaccaga tattgcaccg cgggatagac gctgaggctt tgcaccgcga
      421 cgaaattgat gccggcgccg tattcgccgg cgactgcgga ggccatcact gcgaacaggc
      481 cgacatgccg cgcgagtcga agccggctgg ctgcagtttc agacattgct gttggactcc
      541 ccgttgcggc gccgcagatt cattgttgca gccagcgaac cggattgtcg caaacctgtc
      601 aaatccgaaa atgcagcgca cagccgggac ccgccgcacc gttccgggtg ctcgggccac
      661 ctcccggttg cacgaggcag cggtgccccg gcacgctcag gtttggctgt gcgtcaggtc
      721 agcacgcgat ccgcgtcgcg cagcaccacg tagatcgccg ggatcaccag cacggtcagc
      781 agcgtggacg acgccaggcc gaacagcagc gagatggcca ggccctggaa gatcgggtcg
      841 gtcaggatgg tggccgcgcc gatcatcgcc gccagcgcgg tcagcaggat cggtttgaag
      901 cggatcgccc cggcattcag caataccgcg cgcaaggtct tcccttggcc cgcgccgtgg
      961 cggacgaaat ccaccagcag gatggaattg cgcacgatga tccccgccag cgcgatgaag
     1021 ccgatcatcg aggtggcggt gaagggcgca tggaacagcc agtgccccag caggatgccg
     1081 atcagcgtca gcgggatcgg cgtcaggatg accagcggca gtttgaagct gcggaactgt
     1141 gccaccacca ggatgtagat gcccagcatc gccaccatga aggcggcgcc catgtcgcgg
     1201 aaggtgacgt aggtgatctc ccattcgcca tcccacagca gcgtaggatg cgtttcatcc
     1261 gtcggctggc cgcgcaggct gatcaccggt ttcggcagct tgccccagtc gtgctgcgcc
     1321 accagcttgt ccaccgccag catgccgtag atcggcgcct cgtattcgcc ggccagttcg
     1381 cccatcacca tgtcggcgaa atgcccgtca cggcggaaca gcacgcggga ccccagctcc
     1441 tgctgcgccc gcacgacctg gccgagttcc accacggtct tgctgccggg cagcgtgttg
     1501 gcgggaaccg gcgtggccgc cagcatctcg ttccagctca tgtcgcgctt cggcaggccg
     1561 acgacgatct cgatcgggtc gcgttcggcg ccgcgatgcg aatagccgac cggcacgccg
     1621 ccgaacagcg cctggatggt gtcgtacacg tcgctctgtt cgacgccgaa gtattccagt
     1681 tcgtcctggt cgatgctgat gcgcagccgc ggccgcgggt ggccccagga atcggtgacg
     1741 tccacgatgt acggcaccga cttgaacagc gtcttcacct cctgcgcgac cgccaggcgg
     1801 gtcttcgcgt ccggtccgta gatttccgcc agcagcgtcg ccagcaccgg cgggccgggc
     1861 ggcacttcca ccacctgggt ggtggcgccg gccggcaggt gcagcgcctt cagccggttg
     1921 cgcagatcca gcgcgatggc gtggctgctg cggtcgcggt ccgccttgtc ggcgaggttg
     1981 atctgcagat cgcccagctc cggcgactgg cgcatgtaat agtgccgcac caggccattg
     2041 aagttgaacg gcgcggcggt gccggcatag gcctgcacgg aacgcacctc cggcacggct
     2101 tcgacgatgc gtgcggcggc gaacagcgtg cgctcggtgt cttccaggct gctgccttcc
     2161 ggcaggttca gttccagcgc cagctccgac ttgttgtcga agggcagcag cttcaccgtg
     2221 acgtcctcgg tgtagaacag cgcgcaggcg gcgatggtgc cgaagccgac cagcagcagg
     2281 aaggtccagg cgcgcgcgcg ggttttcagg atgggtgtcg cgaccgcgcg gtagatgcgt
     2341 cccaggcggc cttcgtgatg cccggccata tcgtgttcga taccgggggc ggaggcgggc
     2401 agccactcca cttcctcatc cagccgggcc gcgccgccgg gttcgggtgc cagcttgcac
     2461 agcagccagg gcgccaccac caccgcgacg aagaaggaga acagcatcgc cgcggaggcg
     2521 ttggccggga ttggcgccat gtacggcccc atcaagccgg acacgaacag catcggcagc
     2581 agcgcggcga cgacggtcag cgtggcgacg atggtgggat tgccgacttc cgacaccgcc
     2641 tccaccgcgg cgcgcaggcg ggagcggcgg tcgcgcatcg cccagtggcg ggcgatgttc
     2701 tccaccacca cgatggcgtc gtccaccagg atgccgatgg cggagatcag cgcgaacagg
     2761 ctgacgcggt tgatggtgta acccatcaac ttggcggaga acagcgtcag caggatggtg
     2821 gtggggatca ccaccagcgt caccaccgct tcccgccagc cgatcgccag ggcgatcagc
     2881 accacgatgg agacggtggc gagcgccaga tggaacagca gctcgttcgc cttgtcgttg
     2941 gcggtccggc cgtagtcgcg cgtcacctgt acgcgcacgt cggatgggat cagccggccc
     3001 ttcagcccat ccacccgctg cactatgtcc tgtgcgacca ccaccgcatt ggccccgccg
     3061 cgcttggcca gcgcgatggt taccgccggt tcgcgttgcc agcctggctt gccgggcgtg
     3121 atgtcccaca cccggctgtc gctggtattg gcgccgacga tcaccctggc gacgtcccgc
     3181 acataaaccg ggcggccgtc gcgcgtggtg agcagcaaca tgccgatatc cggtacgccg
     3241 cgcagcgtct gcccggccgc gagcgtgcgg gtgacgccgg cgtcgcgtac cgagccggcg
     3301 acgaactcac ggttggcgcc ctgcaccttg gcgatcagct gctgcagcgt gacgccgaac
     3361 agcgacagct tctcagggtc cggttcgacg cggatctgct cgggtccggt gccgaccatg
     3421 tacgtcgtgc cgatattcgg caccttggtg agttcggtgc gcagcttgcc ggcgagatcg
     3481 ta
//
LOCUS       DASSZH010000523         2397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1308206, whole genome shotgun sequence.
ACCESSION   DASSZH010000523 DASSZH010000000
VERSION     DASSZH010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308206"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1887)
                     /locus_tag="VFN42_11425"
     CDS             complement(<1..1887)
                     /locus_tag="VFN42_11425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET8997268.1"
                     /translation="MPPLAIDPSIILESMGDAFYALDPAWRVVYANRRALTFWKLQAS
                     ELVGRVLWEALPQMLGTPNEALLRRAAAERRTLSFELPSPVTGAWVQVTIAPYETGVG
                     VYWRDITERKQTEQTLRSSEEHLRLAQEAAGIGTWDWDIAGDRIHWSPQMFRLLGCEP
                     FDGSAEALFAVWSERLHPDDRDAANANARAHSRNVRPFAQEFRIMRPDGETRWILSCG
                     SVLPDEYGAPRRMLGVNMDITDRKRTEAALEQRVQERTRALQDTLAALQESRTRYAAV
                     FEHAPVDLVFMRVLADGRLLCEDVNPAWTRHTGYTREQVVGRTLDGIFPPNQAALFES
                     HFRRAIETGQPVEYEYSTEFPVGEVTRQAFQVPLRGTDGTVEHVLLTVLDLTETRRIE
                     AQLRQAQKMEAIGQLTGGIAHDFNNLLTAVTGNLELLQRRTSDERGLRYIDTALRAAQ
                     RGGTLTQQLLAYARQQHLSPQPLDVNVILQGMQELLQRSLGGLVQVSLDLDPDVWPGR
                     ADPTQLESMVLNLAINARDAMPDGGLLRIATRNVADAPAGMAPELERGDYVVIAVIDT
                     GSGMSRDVQERAFEPFFTTKEVGKGSGLGLAQVFGLARQFGGTVRLLSRVGHGTTVEI
                     FLPRS"
     gene            2181..>2397
                     /locus_tag="VFN42_11430"
     CDS             2181..>2397
                     /locus_tag="VFN42_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997269.1"
                     /translation="MDKMTAGLLGAVAGLATMGAAQAATTPAVSPSNALQASSYADLL
                     EPIPNAAALLKADDGLRAEQMNTSTSFQ"
BASE COUNT          387 a          827 c          786 g          397 t
ORIGIN      
        1 cgaacggggc aggaagatct ccaccgtagt gccgtggccg acgcgactga gcagccgcac
       61 ggtgccgccg aattgccgcg ccaggccgaa cacctgcgcc aggccgaggc cgctgccctt
      121 gccgacctcc ttggtggtga agaacggctc gaaggcgcgt tcctgcacgt cccgtgacat
      181 gccgctgccg gtgtcgatca ccgcgatcac cacgtagtcg ccgcgttcca gttccggcgc
      241 catgccggcc ggggcgtcgg cgacgttgcg ggtggcgatg cgcagcaagc caccgtccgg
      301 catcgcgtcc cgcgcattga tggccaggtt cagcaccatg gattcaagct gcgtgggatc
      361 ggcgcggccc ggccacacgt ccgggtccag gtccagcgac acctgcacca atccgccgag
      421 gctgcgttgc agcagttcct gcatgccctg caggatgaca ttcacatcca gcggctgcgg
      481 cgacagatgc tgctgccgcg cataggccag caactgctgc gtcagcgtcc cgccgcgttg
      541 cgcggcacgc agcgcggtat cgatgtagcg cagcccgcgt tcgtcgctgg tgcggcgctg
      601 cagcagttcc aggttgccgg tcaccgcggt cagcaggttg ttgaagtcgt gtgcgatgcc
      661 gccggtcaac tggccgatcg cctccatctt ctgcgcctgg cggagctgcg cctcgatgcg
      721 ccgggtctcg gtcaggtcca gcacggtgag cagcacatgt tccaccgtgc cgtcggtccc
      781 gcgcagcggc acctggaagg cctggcgggt cacctcaccc accgggaatt ccgtgctgta
      841 ttcgtactcg acgggctggc cggtttcgat cgcccggcgg aaatgggatt cgaacagcgc
      901 ggcctggttc ggcggaaaga tgccgtccag cgtgcggccg accacctgtt cgcgcgtgta
      961 gccggtgtgc cgggtccagg cgggattgac gtcttcgcac agcagccggc cgtcggccag
     1021 cacgcgcatg aacaccaggt cgaccggtgc atgctcgaac accgcagcgt agcgcgtgcg
     1081 ggattcctgc agcgccgcca gggtgtcctg caacgcacgc gtgcgttcct gcacgcgctg
     1141 ttccagtgct gcctcggtgc gtttgcggtc ggtgatgtcc atgttgacgc cgagcatgcg
     1201 gcgcggcgcg ccgtactcgt cgggcaggac actgccgcag gacaggatcc accgggtttc
     1261 gccatccggc cgcatgatgc ggaattcctg cgcgaaaggc cgcacattcc tgctgtgcgc
     1321 gcgcgcgttg gcattggcgg cgtcgcggtc atccgggtgc agccgctccg accacacggc
     1381 gaacagcgcc tcggccgatc cgtcgaacgg ttcgcagcca agcagccgga acatctgcgg
     1441 cgaccaatgg atgcggtctc ccgcgatatc ccagtcccag gtgccgatcc cggccgcttc
     1501 ctgggccagg cgcaggtgct cctcactgga acgcagcgtc tgctcggtct gcttgcgctc
     1561 ggtgatgtcg cgccagtaca ccccgacacc ggtttcgtag ggcgcgatgg tgacctgcac
     1621 ccaggcaccg gtgaccggcg acggcaattc gaagctgagg gtgcgccgtt ccgcggcggc
     1681 ccgccgcagc agggcttcgt ttggcgtgcc tagcatttgc ggcagcgcct cccacagcac
     1741 acggccgaca agctcgctgg cctgcaactt ccagaatgtc agtgcgcgcc ggttcgcata
     1801 gacgacgcgc cacgcaggat ccagcgcata gaacgcatcc cccatgcttt cgaggatgat
     1861 acttggatcg atcgcaagcg gcggcacgtc agcccccgag gacgcgggcc tgtcagcgat
     1921 aaccacctcc tcagccttgg ttgaacgaag catgcggcaa tttaccgcga tgcagcacgc
     1981 ccagcaacgc gcggaacatc cgtgaccggc atgcggaaat cggctccgcc gcaggtcacg
     2041 cttccgtggc gtggcccgcg cgggcgcaca tcctgctcac gctccggctg ccgccgaaac
     2101 tttgcaatgc gctccgcgtt gacggaccac tgcaaaagcc gaccggtacg ctctcatagt
     2161 gaaattgagg agacagcctc atggacaaga tgaccgctgg attgcttggc gccgtggcag
     2221 gactcgccac gatgggcgcg gcgcaggccg cgaccacgcc cgccgtgtcg ccatccaacg
     2281 cgctgcaggc gtcgagctat gccgatctgc tcgagccgat tcccaacgcc gctgccttgc
     2341 tgaaggctga cgacgggttg cgggcggagc agatgaacac cagcaccagc ttccaac
//
LOCUS       DASSZH010000524         2844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1478572, whole genome shotgun sequence.
ACCESSION   DASSZH010000524 DASSZH010000000
VERSION     DASSZH010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478572"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..707)
                     /locus_tag="VFN42_11435"
     CDS             complement(87..707)
                     /locus_tag="VFN42_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997270.1"
                     /translation="MPPVSAARPGWFARCFGPARSRSAPTRPPRRAESGKLFTRKAYA
                     NLPPDVRAILNTPLEDCDPALVRLLFAALAQEIAACLPPEAEMTAAQKMYAGLWGDLI
                     AKLAECGLDDAPAEPPPEDDAATALSSAASEPSPESSAPAGAASVFHRGPVSFVAGRA
                     FRQCRRALLQHCAWAFRSLGPPAGRFLPRQQPPPRRLCYAACAGPP"
     gene            complement(1292..1903)
                     /locus_tag="VFN42_11440"
     CDS             complement(1292..1903)
                     /locus_tag="VFN42_11440"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET8997271.1"
                     /translation="MREEIHAYKRERILEEAVKLFYERGFTGTTLDDIAAELGVTKPF
                     IYTHFRSKVELLAALCTPTIEMSLEAVENAAKGPGTPTQRLRRAIVDFTQVILSRQAN
                     IAIFFREEKNLAPEALEEITRLRKAFDRKLSGLLAEGVAAGEFTIGDVSLTALVIGGM
                     ISWGYTWHRPGGRLGIEELAERMAELTLQMVGVRSVAGERLSA"
     gene            2125..>2844
                     /locus_tag="VFN42_11445"
     CDS             2125..>2844
                     /locus_tag="VFN42_11445"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HET8997272.1"
                     /translation="MTLVELSRLQFALTALYHFLFVPLTLGLSVLLAIMESIYVTTGR
                     TIWRDMVRFWGTLFGINFAMGVATGITMEFEFGTNWAYYAHYVGDIFGAPLAIEGLMA
                     FFLEATFIGLFFFGWNRLTRVQHLVVTCLMALGTNLSALWILIANGWMQYPVGAQFNI
                     ATMRMEVTDFWAVVFNPVAQAKFVHTVSAGYVTSAMFVLSISAFYLLRSRYRGLAIRL
                     MTVAASFGLAAALSVVVLGDES"
BASE COUNT          452 a          938 c          980 g          474 t
ORIGIN      
        1 ggcacgaatc aggatagcgg gaccgccatg cggtggcccc gggattgtcg cggcagaagg
       61 acacgccagg gtcggcgtgc gccgggtcag ggcgggccgg cacaagcggc gtagcacagg
      121 cgccgcggcg gcggctgttg gcggggcagg aagcggccgg caggtgggcc cagactgcgg
      181 aaagcccatg cacagtgctg cagaagcgcc cgccggcatt ggcggaacgc ccggccggca
      241 acgaacgata cggggccgcg gtgaaagacc gaggcggcgc cggcgggagc cgaagattcc
      301 ggtgacggtt cggacgccgc cgaggacagg gcagtggcgg cgtcgtcctc cggcggcggt
      361 tcggccgggg catcgtccag gccgcattca gccagcttgg cgatcaggtc gccccacagc
      421 cccgcataca tcttctgcgc cgcggtcatc tccgcctcgg gcggcaggca ggcggcgatc
      481 tcctgcgcga gcgcggcaaa cagcaggcgg acgagggcgg ggtcgcagtc ctcgagcggc
      541 gtgttcagga tggcgcggac gtccggcggc aggttggcat aggccttccg ggtgaagagc
      601 ttgccgcttt cggcccgccg cggcgggcgg gttggcgcgg accgggaccg ggcagggccg
      661 aagcaacgcg cgaaccagcc ggggcgggcg gccgagaccg gcggcatggc atcaggctgg
      721 cgccgggcga ggggcttggg tgcggtggaa cgcggtgcgg gcgtgccaga gccggtggcg
      781 gatggtgggg ctgccgagcg acgcaggcgg gcagccaggc cgatgccgcc ggcaacggcg
      841 ccgcgcaccg tgttgcccag gcggcggagg aaaggcgcaa tggactggtt ggaggttgcg
      901 gaaagaggct cggcagggcg gtgcgcggtt gacatggccg gcgtattagc accacagact
      961 aacgcttgtc aagggaattt tgtctggtag gggaattttt cccactggct acgaactcgc
     1021 ccgggtaacg aaacacgccg gtgggtcata cccgtcgacg ctgatgccga atctgccgtg
     1081 tcatggccgg cgcaggccgg ccacccacgt cttctctcgc ctgtcggcca gagtgcctga
     1141 agagagtttt tccaatctcc agtcatcagg cagaaagtgc gctgtcaaat aacgattctg
     1201 atggcgtctg ctacataacg ccgcaaaagc cgtgggtgcc cgcgccaagc gcgggcatga
     1261 cacatggggg cgcgcattgc gcccacgccc cctacgcgct cagccgctcg ccggcgacac
     1321 tgcgcacccc gaccatctgc agggtcagct cggccatgcg ctccgccagt tcctcgatgc
     1381 ccagccggcc gcccggacgg tgccacgtgt agccccagct gatcatgccg ccgatcacca
     1441 gcgcggtcag gctgacatcg ccgatggtga attcgccggc cgccacgccc tcggccagca
     1501 ggccggacag cttgcggtcg aacgccttgc gcagccgggt gatctcttcc agcgcctccg
     1561 gcgccaggtt cttctcctca cggaagaaga tggcgatgtt ggcctggcgg gacaggatca
     1621 cctgggtgaa gtccacgatc gcccggcgca gccgctgggt cggcgtgccc gggcccttcg
     1681 ccgcgttctc caccgcctcc agcgacattt cgatggtcgg cgtgcacagc gccgccagca
     1741 gctccacctt gctgcggaaa tgcgtgtaga tgaacggttt ggtcacgccc agttcggccg
     1801 caatgtcgtc cagagtggtg ccggtaaagc cgcgctcgta gaacagcttc accgcctcct
     1861 ccaggatgcg ttcccgtttg taggcgtgga tctcttcgcg catgcgacaa ataccggtcc
     1921 ctgaggcaac tgccatgcta ccgctaacgc ggccggaagg aaagcgtacc ccccggtagc
     1981 agggcggtaa gccctgcttc caaagggaat cctgcccagc acataacctg cgacgtccct
     2041 ggaacgactg atctatgtca gggcaccccc cgtccggcgg gtgcatgagg ctggggactc
     2101 tcacgctttg ccgggtgccc agcgatgacc ctcgtcgaac tctcccgcct gcagttcgcc
     2161 ctgacggcac tgtaccactt cctgtttgtc cccctcaccc tcggcctctc cgtcctgctg
     2221 gccatcatgg agagcatcta cgtcaccacc ggccgcacca tctggcgtga catggtgcgg
     2281 ttctggggca cgctgttcgg catcaacttc gccatgggcg tcgccaccgg catcaccatg
     2341 gaattcgagt tcggcaccaa ctgggcgtac tacgcgcatt atgtcggtga catcttcggc
     2401 gcgccgctgg cgatcgaagg gctgatggcg ttcttcctgg aagccacctt catcggcctg
     2461 ttcttcttcg gctggaaccg cctcacccgc gtgcagcacc tggtggtcac ctgcctgatg
     2521 gcgctgggca ccaacctgtc cgcgctgtgg atcctgatcg ccaatggctg gatgcagtat
     2581 ccggtcggcg cgcagttcaa catcgccacc atgcgcatgg aggtcaccga cttctgggcg
     2641 gtggtgttca acccggtggc gcaggccaag ttcgtgcaca ccgtgtcggc cggctacgtc
     2701 accagcgcga tgttcgtgct gtcgatcagc gccttctacc tgctgcgcag ccggtatcgc
     2761 gggctggcga tccgtttgat gacggtggcc gccagcttcg gcctggccgc cgcgctgtcg
     2821 gtcgtcgtgc tgggcgatga gagc
//
LOCUS       DASSZH010000525         2747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_166081, whole genome shotgun sequence.
ACCESSION   DASSZH010000525 DASSZH010000000
VERSION     DASSZH010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166081"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..341)
                     /locus_tag="VFN42_11450"
     CDS             complement(177..341)
                     /locus_tag="VFN42_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997273.1"
                     /translation="MGIAVRQEIAQQTAGNANPVPRESRTHSDELSSYLAVMALVVGL
                     IIAWVCLCGA"
     gene            complement(468..1226)
                     /locus_tag="VFN42_11455"
     CDS             complement(468..1226)
                     /locus_tag="VFN42_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HET8997274.1"
                     /translation="MRIEYLYRYPVKGLTAEALEAAEVEANGCIPWDRAFALAQGDSG
                     FDPAAPTWLMKDNFMCLKKNAKAAALFSFFEPRTGMLAIRAPDGSAVVENALTESGRE
                     RIGAFLTAYLGEEARGAPRFQHVPGHSFCDQRHKVVSLINLASLRDYEARVGDRRHRR
                     RFRANVWFSGAPAWAERSWVGQQLQVGGAVLRVTRPITRCPATEVNPVTAERDAQPVA
                     ELRTLYGHVELGVHAQVVEGGRFGVGDAIELLGE"
     gene            1347..>2747
                     /locus_tag="VFN42_11460"
     CDS             1347..>2747
                     /locus_tag="VFN42_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019088301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HET8997275.1"
                     /translation="MDIEKFTERARGFLQAAGTIAIREFHQRITPEHLLKALLDDEEG
                     AAAGLIRTAGGDPKAALVAVEAEISRQPKVQGPGAGQPNITPELVRVLDAAQQAANKA
                     GDEYVAQDRLLVALAASDTPAARALRQSGATPQALEKAVADIRKGRRVDSANAEATFD
                     ALKKYARDVTALAREGKLDPVIGRDEEIRRTIQVLARRTKNNPVLIGEPGVGKTAIVE
                     GLALRIVNGDVPEALKNKRLLSLDLGAMVAGAKYRGEFEERLKSVLKEIESAQGEVIL
                     FIDEMHTLVGAGRADGAMDASNLIKPELARGTLHCIGATTLDEYRKHVEKDAALARRF
                     QPVFVGEPSVEDTISILRGIKEKYELHHGVRITDGALVAAATLSNRYITDRFLPDKAI
                     DLVDEAASRLRMQVDSKPEELDELDRRVIQLKIEREALKKENDSASRDRLEKLEHELA
                     DLEDKSNAMTAAWKAEK"
BASE COUNT          506 a          906 c          947 g          388 t
ORIGIN      
        1 gatctaccac gaggccggcg ccaccgcgat cttccagtcg catccgtgcg agcggcggtt
       61 cagagacatg aacgcggtgt cgcaacaact gcagggccgg cgcgcgcact tcgaaacggt
      121 cggactctac atgctgggcg gcgacccgcc gctgcggtgg gtgtagcccg ccctcgtcag
      181 gcgccgcaga ggcagaccca ggcgatgatc agcccgacca ccagcgccat caccgcaagg
      241 taggaggaca gttcgtcgct gtgggttcgg ctttcccgcg gcacggggtt ggcgttaccg
      301 gcggtctgct gggcgatttc ctggcgcact gcgatcccca cgctgcttgc ggtccactga
      361 cgggcgggga cacaggtaga gcgcgaagat gcgagcggac aaccgcaaac tcacggatgc
      421 gcgatcacaa attcagcgct gaactgtatc gtttccacgc tgccgcttca ttctcccagc
      481 agttcgatcg catcgccgac gccaaaccgg ccgccctcca ccacctgggc atgcacaccc
      541 agctccacgt gcccgtacag cgtccgcagc tcggccaccg gctgcgcatc ccgttcggcg
      601 gtgacaggat tcacctcggt cgccgggcag cgggtgatcg gccgggtgac ccgcagtacc
      661 gccccgccca cctgcaattg ctggccgacc caggaccgct ccgcccaggc cggggcgccg
      721 ctgaaccaga cattggcgcg gaaccgccgg cggtgccgcc ggtcaccgac cctggcctca
      781 taatcgcgca ggctggccag gttgatcagg ctgaccacct tatgccgctg atcgcagaaa
      841 ctatggccgg gcacgtgctg gaagcgcggg gcgccgcggg cctcctcgcc cagatacgcg
      901 gtcaggaagg cgccgatccg ctcccggccg gattcggtca gcgcgttctc caccaccgcc
      961 gaaccgtccg gcgcgcggat cgccagcatg ccggtgcgtg gctcgaaaaa cgagaacagc
     1021 gccgccgcct tggcattctt cttcaggcac atgaagttat ccttcatgag ccaggtcggg
     1081 gccgccgggt cgaagccgga atcgccctgc gccagggcaa aggcgcgatc ccacgggatg
     1141 cagccgtttg cttcgacttc ggcggcttcc agagcttcgg cggtcaaccc cttgaccggg
     1201 tagcggtaga gatactcgat gcgcatgcgg cacccccttg agtcgcagtg tgccccgacc
     1261 cacctgtttc ggcaaaggtt cgcaattccg ttgcctcctg aggggcggag ccgggaccgg
     1321 gtcgcctacg aacagggaca ctgagcatgg atatcgagaa attcactgag cgggcgcgcg
     1381 gcttcctgca agctgcgggt acgattgcca tccgcgaatt ccaccagcgg atcacgccgg
     1441 aacacctgct gaaggcgctg ctggatgacg aggaaggggc ggcggcgggg ctgatccgca
     1501 ccgcgggcgg tgatccgaaa gccgcgctgg tggcggtcga agccgagatc agccgccagc
     1561 cgaaggtgca gggcccgggc gccgggcagc cgaacatcac gccggagctg gtgcgcgtgc
     1621 tggatgccgc gcagcaggcc gccaacaagg cgggcgacga gtacgtggcg caggaccggc
     1681 tgctggtcgc actggccgcg tccgacacgc cggcggctcg ggcattgcgg cagagcggcg
     1741 cgacgccgca ggcgctggaa aaggcggtgg ccgacatccg caagggacgc cgcgtggatt
     1801 ccgccaatgc cgaggccacg tttgacgcgc tgaagaaata cgcccgcgac gtgacggcgc
     1861 tggcgcggga aggcaagctc gaccccgtca tcgggcgcga cgaggagatc cgccgcacca
     1921 tccaggtgct cgcccgccgc accaagaaca acccggtgct gatcggcgaa cccggcgtcg
     1981 gcaagaccgc gatcgtcgaa ggcttggcgc tgcgcatcgt caatggcgac gtgccggagg
     2041 cgctgaagaa caagcgcctg ctgtcgctcg acctcggcgc catggtggcc ggcgcgaagt
     2101 atcgcggcga gttcgaggaa cggctgaagt cggtactgaa ggagatcgag tccgcgcagg
     2161 gcgaggtcat tctgttcatc gatgagatgc acacgctggt cggcgcggga cgggcagacg
     2221 gggcgatgga tgcctccaac ctgatcaagc cggagctggc gcgcggcacg ctgcactgca
     2281 tcggcgccac cacgctggac gagtatcgca agcacgtgga gaaggacgcc gcactggcgc
     2341 gccgtttcca gccggtgttc gtcggcgagc cgagcgtgga agacacgatc agcattctgc
     2401 gcggcatcaa ggagaagtac gagctgcacc acggcgtgcg catcaccgac ggcgcactgg
     2461 tggcggcggc gacgctgtcc aaccgctaca tcaccgaccg gttcctgccg gacaaggcga
     2521 tcgacctggt ggacgaggcg gccagccggc tgcgcatgca ggtggacagc aagccggagg
     2581 agctggacga gctcgaccgg cgcgtcatcc agctgaagat cgagcgtgag gcgctgaaga
     2641 aggaaaacga ttccgcctcg cgcgaccggt tggagaagct ggaacatgag ctggccgacc
     2701 tggaagataa gtcgaacgcg atgaccgccg cctggaaggc cgaaaag
//
LOCUS       DASSZH010000526         3047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1563748, whole genome shotgun sequence.
ACCESSION   DASSZH010000526 DASSZH010000000
VERSION     DASSZH010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563748"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..615
                     /gene="fdxA"
                     /locus_tag="VFN42_11465"
     CDS             283..615
                     /gene="fdxA"
                     /locus_tag="VFN42_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HET8997276.1"
                     /translation="MTYVVTENCIRCKYMDCVEVCPVDCFYVGENMLVIHPDECIDCG
                     VCEPECPAEAIVPDSDDKAGPWLEQNRTYAALWPNITRKGPPPEDADEWKDKPGKAQY
                     FSAEPGKP"
     gene            1272..1943
                     /locus_tag="VFN42_11470"
     CDS             1272..1943
                     /locus_tag="VFN42_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HET8997277.1"
                     /translation="MPQKPAVHAEPSQPPVPNREAESTATEESGAAAPRPAESGRRPP
                     GMPPGPPVLTPRMPAKAEIFHEGDHVVYPTHGVGKVERIAVEEIAGHKLELIHITFEE
                     NRMTLRVPVAKARSAGLRKLATRKLFDEAMNVLKGKARIKRTMWSRRAQEYEAKINSG
                     DPLAIAEVVRDLHRNAGQPDQSFSERQIYEAAMDRLAAELAALDKTDKMTASTKLTDF
                     LKTIA"
     gene            2058..3038
                     /locus_tag="VFN42_11475"
     CDS             2058..3038
                     /locus_tag="VFN42_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HET8997278.1"
                     /translation="MRIHLQNPADDPLFEFTLPMWQAALARAADPGGAHSVSLGQTPA
                     EFAAAMREAEALVCDVSVVKAQFPCDAPRLKLLFLTNAGLDRLAPFDWLPPGVALLNN
                     RGTHLVKSGEFGIMAVLMLVNRVPQMVTHQRAGRWQKLWGSVLAGKRLTVVGLGTLGG
                     AVAEHAARFGMQVTGVRAHPAPHPACGAVLGTAELDAVLPHTDVLVLACPLTAATRNI
                     MDRRRLALLPAGAGVVNIGRGELADQDALCDLLDSGHLSGAVLDVFDPEPIPPGHRLW
                     TTPNLVISPHTAADDPATYNPRSLDIFLENVAAWRAGRPLPNRFDIARGY"
BASE COUNT          509 a         1047 c         1055 g          436 t
ORIGIN      
        1 cgccggatgg cgtacggccg gtgaccagca ggaaattgct catattgatg ccggcgcgca
       61 gcacgccgtg cggggcaagt tgggacacta tgtccgggga catgggctgc tctcctggat
      121 cggttgcggg cagtgtgcgg cggcttgcgg cctccggcaa gcggctgccg gcgccggcag
      181 gggggcgatc ttgcgccctg gggcaaaccc cggcataccc cgcgcgactg ttttcaggcg
      241 ccgcggcatc cgtcgcgcgg cgtactgttg ccgggggctc gcatgaccta cgtcgtcacg
      301 gaaaactgca tccgctgcaa gtacatggat tgcgttgaag tctgtcccgt cgactgcttc
      361 tatgtcggcg agaacatgct ggtgatccac ccggacgaat gcatcgattg tggcgtttgc
      421 gagccggaat gcccggctga ggccatcgtg ccggacagcg acgacaaagc gggtccgtgg
      481 ctggaacaga accgtactta cgcggcgctg tggccgaaca tcacccgcaa gggtccgccg
      541 cccgaggatg ccgacgaatg gaaggacaag ccgggcaagg cgcagtattt ttccgccgaa
      601 ccaggaaaac cctgatcaca ccgggggtta cccccctgac gtaacacgcg tgtgatgcta
      661 tattagctcc gggcgacgcc ttgcctgggt ggggatctgt cgccaatcca ttcggagtcg
      721 atccgccttg ggggtgggag cgcgccggct gacgctgtgc cggcacgatg accccatgcg
      781 cgggtcgtgg attcggagct tgtgaggtac cagggatgaa ggcatccgcc gggactgcga
      841 aatcaggccg gaccgccggg cagacccgct cggcagccgc gaagccggcc gcgccacggt
      901 cgcaagccaa atcggttgcc gttgccgtga agaaggcgcc gcccacgcgg ccggcagcag
      961 ccggcaagcc gccgaagcag ccggccgccg ccgccgctgc cgccaagccg gccgcgaagg
     1021 ccgaggtgat tgccaagccg gccaaacccg aggctgagaa gccgaccgcc gccagaacgg
     1081 ccgcaccgaa accggccgct ccgaagccgg ctgcaccgaa gccggccgcc aagcccagct
     1141 tggtggcgcc ggtcgaaacc aaatctgctg cgccggcacc tgctgcgccg gcgcctgcgg
     1201 ccccagcgcc gtccaggccg gtcgcatcgg caccctccga aacgccttcc gctccaactg
     1261 tcgagaaatc cgtgccgcaa aagcctgccg tccacgccga acccagccag ccgcctgtgc
     1321 cgaatcggga ggcagaatcg accgccaccg aggaatcggg cgccgccgcg ccccgcccag
     1381 ccgaatcggg ccgccggccg ccgggcatgc cgccgggacc gccggtgctg accccgcgca
     1441 tgccggccaa ggccgagatc ttccatgaag gcgaccatgt ggtctacccg acccatggcg
     1501 tcggcaaggt ggagcgcatc gccgtggagg agattgccgg ccataagctg gagctgatcc
     1561 acatcacctt cgaagagaac cgcatgacgc tgcgggtgcc ggtcgccaag gcacgcagcg
     1621 ccgggttgcg caagctggcc acgcgcaagc tgttcgatga ggcgatgaac gtgctgaagg
     1681 gcaaggcacg catcaagcgc accatgtggt cgcgccgtgc ccaggaatac gaggcgaaga
     1741 tcaattccgg cgacccgctc gccatcgccg aggtggtgcg agacctgcac cgtaacgccg
     1801 gccagcccga ccagagcttc tccgagcggc agatctacga agccgcgatg gaccgcctgg
     1861 ccgccgaact cgcggcgttg gacaagaccg acaagatgac ggcatccacc aagctgaccg
     1921 acttcctgaa gaccatcgcc tgacctggga attccctctc cgcccctggg agcggggagg
     1981 gccagggtga aggatcggag ctctccgcgc ctgccagtgg ctgggcaacc ggaacgcttg
     2041 atggcacgct gcccggcatg cgcatccacc tgcagaatcc cgccgacgat ccattgttcg
     2101 agttcaccct gcccatgtgg caggccgcgc tggcgcgggc cgcggatccc ggcggcgcgc
     2161 acagcgtcag cctgggccag acgcccgccg aattcgccgc cgcgatgcgc gaggccgagg
     2221 cgctggtctg cgatgtcagc gtggtcaagg cgcagttccc ctgcgatgcg ccgcggctga
     2281 agctgctgtt cctgaccaat gccggcctgg acaggctggc gccgttcgac tggctgccgc
     2341 ccggcgtggc gctgctgaac aatcgcggca cgcatctggt gaagtccggc gagttcggca
     2401 tcatggcggt gctgatgctg gtgaaccgcg tgccgcagat ggtgacgcat cagcgtgccg
     2461 gccgttggca gaagctgtgg ggctcggtgc tggccggcaa gcgcctgacg gtggtcgggt
     2521 tgggcacgct gggcggcgcc gttgcggagc atgccgcccg gttcggcatg caggtgaccg
     2581 gcgtgcgcgc gcatcccgcg ccgcatccgg cctgcggcgc cgtgctcggc accgcggagt
     2641 tggacgcggt gctgccacac accgacgtcc tggtgctggc ctgcccgctg accgcggcga
     2701 cgcgcaacat catggatcgc cgccgcctgg cgctgctgcc ggccggcgcg ggcgtggtga
     2761 atatcggccg cggcgaactg gccgatcagg acgcgctgtg cgacctgctg gacagcggcc
     2821 acctgtccgg cgcggtgctg gacgtgttcg acccggaacc gatcccgcct ggccaccggc
     2881 tgtggaccac gccgaacctg gtcatcagcc cgcataccgc ggcggacgat ccggcgacct
     2941 acaatccgcg cagcctggac atcttcctgg agaacgtggc ggcctggcgg gcgggacggc
     3001 cgctgcccaa ccgcttcgac attgcccgcg gctattgagg gaagccc
//
LOCUS       DASSZH010000527         6029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_967235, whole genome shotgun sequence.
ACCESSION   DASSZH010000527 DASSZH010000000
VERSION     DASSZH010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967235"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /gene="glpK"
                     /locus_tag="VFN42_11480"
     CDS             <1..1185
                     /gene="glpK"
                     /locus_tag="VFN42_11480"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007398014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HET8997279.1"
                     /translation="ALVWQDTRTDSLVAEYARDGGQDRFRSQTGLPLASYFSALKLRW
                     LLDNVPDARRRAEAGDLAFGTIDTWLMWHLTGQHVTDVTNASRTLLMDLATLEWHDGL
                     LSAFGIPRAVLPRIVASSAPYGTARAPLEGVPVAGILGDQQAALLGQACCEPGEAKNT
                     YGTGCFLLLHTGTTPKPSSHGLVTTVAAKFGDAPASYALEGSIAITGALVQWLRDNLG
                     VIRSSAEVETLAASVPDNGGVYFVPAFSGLYAPYWRADARGVIAGLTRFATAGHIARA
                     ALEAAAYQTLDVVRAMEQDSGIPIRTLRVDGGMVVNDTLMQFQADMLNVAVARPACIE
                     TTALGAAYAAGLAVGYWHGLDDLRANWAEARRWSPHMPQDRRTALIAGWHKAIERSFN
                     WA"
     gene            1328..1528
                     /locus_tag="VFN42_11485"
     CDS             1328..1528
                     /locus_tag="VFN42_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997280.1"
                     /translation="MPAFAGMTEFGVPKSLAHRRLVLRGRLRDVEGGGNIPADGAAVP
                     SAIRSFSGVRSGGCQARRAKRA"
     gene            complement(1624..2730)
                     /locus_tag="VFN42_11490"
     CDS             complement(1624..2730)
                     /locus_tag="VFN42_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006559948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET8997281.1"
                     /translation="MRYTGAVLAAALLATPLAAQAQPVQGPYVSAGVGLDLPQNVQAT
                     PYPSTPGFIGTHLRMNEHLGLDALGSAGYAIGGGFRFEIEGDYRQNGLNGISRTPFPT
                     SVSGDVHTYGVMVNALYDMDIGLPWLYPYLGAGVGYQWTTLNNVRAAQSGGPFRFGTN
                     ETQGALAFQGILGLSFPIPNMPGLSATAEYRFMDITGGERFAGTQVTSAGGAATPVSF
                     ELHNQYNHMFLLGVRYAFYTPPPPPLAPATPAAAPAPAVQPARSYLVFFDWDKATLTE
                     RARQIIKEAADNSTHVQYTRIAVNGYTDTSGSPQYNKGLSVRRADAVAAELVRDGVPR
                     NAIAIKGFGETHLLVPTGANVREPQNRRVEIIIK"
     gene            complement(2920..4026)
                     /locus_tag="VFN42_11495"
     CDS             complement(2920..4026)
                     /locus_tag="VFN42_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008853272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET8997282.1"
                     /translation="MTFRGALLAAAMLALPVAASAQPVTGPYISLGAGVNLMQDEHMS
                     GMSVAGGGVAGASGNLDLHPGPAFVAAMGWGFGNGLRAEIEGNYRYNAFYGSSGFGPA
                     VTHFGGHEQKFGGMVNVLYDFVGLTPMLQPYVGAGIGYEGVSEQNLHSFGDTAVFSGS
                     NSTKGSFAYQVILGAAVPIQAMPGLALTAEYRFMGLVGDRSYGGTGTALSPLRTGAAS
                     VTMNDDFNHTFLIGVRYNFGQAPAPAPMAAPAAAPAPAVQPARSYLVFFDWDKATLTE
                     RARQIIHEAAENSTRVQYTQIAVNGYTDTSGTAKYNMGLSIRRAQAVAAELVRDGVPR
                     NAIVIKGFGETHLLVPTGEGVREPQNRRVEIIIR"
     gene            complement(4195..4287)
                     /locus_tag="VFN42_11500"
     tRNA            complement(4195..4287)
                     /locus_tag="VFN42_11500"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4251..4253),aa:Sec,seq:tca)
     gene            complement(4306..5667)
                     /gene="selA"
                     /locus_tag="VFN42_11505"
     CDS             complement(4306..5667)
                     /gene="selA"
                     /locus_tag="VFN42_11505"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HET8997283.1"
                     /translation="MLHATSPLPSLDALLRRPESAVLLERFGRQPLIQALRDILAAHR
                     AARTFGQLPEAILDETADLLARQSIASQRPVFNLTGTVLHTNLGRAPLPPEAADAAAE
                     ALRSATTLEFDLDSGRRGERDDHIAPLLCALTGAEAATVVNNNAAAVLLTLNTFALGR
                     EVPVSRGELIEIGGSFRIPDIMARAGAVLREVGTTNRTHLKDYQAAIGPQTGALMRVH
                     PSNYAITGFTAAVPTPELAAIARAAGVPLIEDLGSGSLIDLTAWGLPHEPTPRESLAA
                     GADLVTFSGDKLLGGPQAGLIVGHIPLIQRLNANPLKRALRLDKARLAALEQVLLLYR
                     DPDRVRLRLPTLRLLTRPETEIRATAERLRGPLQQAFPARPVTVEPCRSQIGSGAMPV
                     DLLPSAALAIGGNQLDSLARFLRRLPRPVIGRIVRGQLLLDCRCLEPEQEADFIRQLP
                     VPA"
BASE COUNT         1022 a         2001 c         2051 g          955 t
ORIGIN      
        1 gcgctggtct ggcaggacac ccgcaccgat tcgctggttg cggagtacgc gcgcgatggc
       61 ggtcaggacc gcttccgcag tcagaccggt ttgccgctcg ccagctactt ttccgcactg
      121 aaactgcgct ggctgctgga caacgtaccc gacgcgcgcc gccgcgccga ggcaggcgac
      181 ctggcattcg gcaccatcga cacgtggctg atgtggcacc tgacgggaca gcacgtcacg
      241 gacgtcacca atgccagccg taccctgctg atggaccttg ccacgctgga atggcacgac
      301 ggcctgctgt ccgccttcgg cattccgcgc gccgtgctgc cgcgcatcgt ggcgtcatct
      361 gcgccgtacg gcacagcaag ggcgccgttg gaaggtgtcc ccgtcgccgg catcctgggc
      421 gatcagcaag ccgcgctgct cggccaggcc tgctgcgaac cgggcgaggc gaagaacacc
      481 tacggcaccg gctgcttcct gctgctgcac accggcacca ccccgaaacc atccagccat
      541 ggcctggtca cgacggtcgc ggcgaagttc ggcgatgcgc cggcgagcta tgcgctggaa
      601 ggctccatcg ccatcaccgg cgcgctggtg caatggctgc gcgacaatct tggggtgatc
      661 cgcagcagcg cggaggtgga gacgctcgcg gcgagcgtgc ccgacaatgg cggcgtgtat
      721 ttcgttcccg cgttctccgg gctgtatgcg ccatactggc gtgccgatgc gcgcggggtg
      781 atcgccggac ttacccgctt cgccactgcc ggccacatcg cccgtgccgc gctggaagcc
      841 gccgcctacc agacgctgga tgtggtgcgc gcgatggagc aggattccgg catcccgatc
      901 cgcacgctgc gcgtcgatgg cggtatggtg gtgaacgaca cgctgatgca gttccaggcc
      961 gatatgctca acgtggccgt cgcgcgtccc gcgtgcatcg aaaccacggc actgggcgcc
     1021 gcctacgcag ccggactggc ggtcgggtat tggcacggcc tggacgattt gcgcgccaat
     1081 tgggcggagg cacgccgctg gtcgccgcac atgccgcaag accggcgcac ggcgttgatt
     1141 gccggctggc acaaggccat cgagcgatcg ttcaactggg cgtaacgcag cgctacgcgg
     1201 cgtccgccaa ggcattcccg cgttatgcca acccgccgag caagtgacac accgggcgca
     1261 gtaccgcgtc gtggcgggcg acggcccgcc acccacgact ttgctgcgca actaaggaaa
     1321 gtcgtagatg ccggccttcg ccggcatgac ggaattcggc gtgccgaaga gtctagctca
     1381 tcgtaggttg gtattacgtg gccgccttcg tgacgtcgaa ggcggcggca acattcctgc
     1441 tgatggcgca gccgtgccga gcgcgatacg cagcttctcc ggcgtgcggt ccggcggctg
     1501 ccaggcacgc cgtgcgaagc gggcctgaat tcacagatcc gcgccgcacg gattggcatc
     1561 gaagcgtcgg cctacgcgcg gggcgatgcc cgccgcgcag gccggccacg tcgttccgaa
     1621 tcgctacttg atgatgatct cgacgcgacg gttctgcggt tcacgcacat tcgcgccggt
     1681 cggcaccagc agatgcgtct cgccgaaccc cttgatggcg atggcgttgc gcggaacgcc
     1741 gtcacgcacc agctccgccg ccaccgcatc cgcgcggcgc accgacagcc ccttgttgta
     1801 ctgcggcgaa ccggaggtgt cggtgtaacc attcaccgcg atccgggtgt attggacatg
     1861 ggtcgagttg tccgctgcct ccttgatgat ctgccgcgcg cgctcggtca gcgtcgcctt
     1921 atcccagtcg aagaacacca ggtaggaccg cgcgggctgc actgccgggg ccggtgccgc
     1981 ggccggcgtc gccggagcca gcgggggcgg cggaggcgtg tagaaggcgt agcgcacgcc
     2041 caacaggaac atgtggttgt actggttgtg cagttcgaaa ctgaccgggg ttgccgcgcc
     2101 gcccgcggag gttacctgcg tgccggcgaa gcgctccccc cccgtgatgt ccatgaaccg
     2161 gtattccgcg gtggcggaga ggccgggcat attcgggatc gggaaggaca ggcccagtat
     2221 gccctgaaac gccagcgcgc cctgcgtctc gttggtgccg aaccggaaag gcccgcccga
     2281 ctgtgccgcg cgcacgttgt tcagggtcgt ccactgatag ccaacgccag cgcccaggta
     2341 ggggtacagc caaggcagcc cgatatccat gtcgtagagc gcgtttacca tcaccccgta
     2401 ggtgtgcacg tcgccgctta ccgaggtggg gaaaggcgtg cggctgatcc cgttgaggcc
     2461 gttctggcgg taatcgcctt cgatctcgaa gcggaacccg ccgccgatcg catagccggc
     2521 gctgcccagc gcatccagac ccaggtgctc gttcatccga agatgcgtac cgataaagcc
     2581 aggcgtggat ggatagggtg tcgcctgcac gttctgcggc aggtccagcc cgacaccggc
     2641 actgacatag gggccctgaa ccggttgggc ctgcgctgca agcggtgtgg ccagcagggc
     2701 tgccgccagg accgcgcccg tgtatcgcat gcgtctgtct cctcaagcgc cgttcattcg
     2761 gtcgggaagc gcgtgtaccc caccggcagc aggactggga cggaagggtg ttggacaatc
     2821 accggaattg agtcacgcca gcgtgctcca ccgacggttc aaatgcaaaa cagggcgccc
     2881 ggaagcgccc tgccttgtcg cacggttggt cgaacccggt tagcggatga tgatctccac
     2941 gcgccggttc tgcggctcac gaacgccctc gccggtcggc accagcaggt gcgtttcgcc
     3001 gaagcccttg atgacgatcg cattgcgcgg cacgccatca cgcaccagtt ccgcggctac
     3061 tgcctgcgcg cggcggatcg acaggcccat gttgtacttg gccgtgccgg aggtgtcggt
     3121 gtagccgttc acggcgatct gggtgtactg cacgcgggtc gagttctccg ccgcctcgtg
     3181 gatgatctgg cgggcacgct cggtcagggt cgccttatcc cagtcgaaga acaccaggta
     3241 ggaacgggcg ggctgcacgg ccggtgcagg cgcagcggcc ggggcggcca tcggtgccgg
     3301 cgccggggcc tggccgaagt tgtagcgcac accgatcagg aaggtgtggt tgaagtcgtc
     3361 gttcatcgtg acgctggccg cgccggtgcg cagcggggac agcgccgtgc cggtgccgcc
     3421 atagctccgg tcgccgacca ggcccatgaa gcgatactcg gcggtgagtg ccaagccggg
     3481 catcgcctgg atcggaactg cggcgcccag gatcacctgg taggcgaagc tgcccttggt
     3541 cgagttactg cccgaaaaca ctgccgtgtc gccgaagctg tgcaggttct gctcggacac
     3601 gccttcatag ccgataccgg cgccgacata gggctgcagc atgggggtca ggccgacgaa
     3661 gtcgtacagc acgttcacca tgccgccgaa cttctgctcg tgaccgccga aatgggtcac
     3721 cgccgggccg aagccgctgc tgccgtagaa ggcgttgtac cggtaattgc cttcgatctc
     3781 ggcgcgcaga ccgttgccga aaccccaacc catggcggcg acgaaggccg ggccaggatg
     3841 caggtcaaga ttgccggagg cgcccgcgac gccgccaccc gcgacggaca tgccggacat
     3901 atgctcgtcc tgcatgagat tcacgccggc accgaggctg atatatggtc cagtgaccgg
     3961 ctgggcgctt gccgcgacgg gcaaagccaa catcgcggcc gctaggagtg caccccgaaa
     4021 cgtcatcgtc acttctcctc gttcgctgtc gcgggaccgg tccgcacacg cggaccatat
     4081 gccctttcgc tgttaaaatc ttagcgcttg cacaatgcga aaacgagagg gaggcggatc
     4141 atttggaaag cgatgtggcg gaaaaatcac agcgcctggt acccccgcca caggcggaag
     4201 agagcaggag tcgaacctgc ccgggaccgc tcggcagccc caaccgggtt tgaagtccgg
     4261 ccgacccacc ggggccgctt ctcctccgcc gccattatgg aagggtcacg ccgggaccgg
     4321 aagctgccgg ataaaatcgg cttcctgttc gggctccagg cagcggcaat ccagcagcaa
     4381 ctgaccgcga acgatgcggc cgatcaccgg ccggggcaac cgcctcagga atcttgccag
     4441 tgaatccaat tggttaccgc caatggccag tgccgcggag ggcagcagat cgaccggcat
     4501 ggcgcccgac ccgatctggc ttcggcacgg ttcaaccgtc accgggcggg cggggaatgc
     4561 ctgctgcagc gggccacgca gccgctccgc ggtggcgcgt atctcggtct ccgggcgggt
     4621 cagcagccgc agcgtgggca gccgcagacg cacccgatcg gggtcgcggt aaagcagcaa
     4681 cacctgctcc agcgccgcca gccgagcctt gtccaggcgc aacgcccgtt tcagcgggtt
     4741 ggcgttgagc cgttggatca acggaatgtg accgacgata agcccggcct gcggcccgcc
     4801 cagcagcttg tcgccggaga acgtcaccag gtcggcacca gccgccaggg attcgcgcgg
     4861 agtcggttcg tgcggcagcc cccacgcggt caggtcgatg aggctgccac tgcccaagtc
     4921 ctcgatcagc ggcacaccgg cagcacgggc aatcgccgcc agttccggcg tgggtacagc
     4981 ggcggtgaag ccggtgatgg cataattgga gggatgcacc cgcatcagag cgccggtctg
     5041 cggcccgatc gccgcctggt agtccttcag gtgcgtccgg ttggtggtgc cgacctcccg
     5101 cagcaccgct ccggcgcggg ccatgatatc gggaatccgg aaggagccgc cgatctcgat
     5161 cagctcgccg cgcgacaccg gcacttcgcg gccgagggca aaggtgttga gcgtgagcag
     5221 gacggccgca gcgttgttgt tcaccacggt ggcggcttcc gcgccggtca gcgcgcatag
     5281 caggggggca atatggtcgt cgcgctcacc gcgccggccc gagtcgagat cgaattccag
     5341 cgtggtggcg ctgcgcagcg cttcggcagc ggcatcggcg gcctcgggcg gcagcggcgc
     5401 tcggccgaga ttggtatgca gcaccgtgcc ggtcaggttg aacaccggac gttgcgaggc
     5461 gatggattgc cgggccagca gatccgccgt ctcgtcgaga atggcttcag gtaactggcc
     5521 gaacgtgcgc gcggcgcgat gcgccgcaag aatgtcgcgc agcgcctgga tcagcggctg
     5581 gcgaccaaag cgctccagca gcacggcgct ttccggccgg cgcagcaggg catcgagcga
     5641 gggaagcggg ctggtggcgt gcagcatggc gcgcgtccaa cccgactaga gaaccgcaac
     5701 cacggatgaa caccgatgaa caccgatggc gccgcgcttc ccgaccgcct ggaggaaatc
     5761 cccgccaaag gccgaaattg tcctcgccgt ggctgaacca ggccgcgcca ccccgctggg
     5821 gtccgccggg agccatccgt gtacatccgt ggtcatgcgc ggttccaatt ccctgatcgc
     5881 gttcggagcc taacgcaagt cgccggacgt gacagcgcgg caaagccaag aagccgcaca
     5941 acgggagcag ccggcgagcg gtcgctccct gcggcgccct tgcacgatgc cagcggtgac
     6001 tgccagcggc ggtgcttcgg cgcgggttc
//
LOCUS       DASSZH010000528         9912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1581026, whole genome shotgun sequence.
ACCESSION   DASSZH010000528 DASSZH010000000
VERSION     DASSZH010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 9912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9912
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581026"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..1019
                     /locus_tag="VFN42_11510"
     CDS             234..1019
                     /locus_tag="VFN42_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HET8997284.1"
                     /translation="MSLSGKTALVTGSTSGIGLGIARVLAGQGANLMLNGFGDAAEIE
                     TLRAELEREHKIRVAYHPADMSKPAQIAAMVAVAAEQLGGVDILVNNAGIQHVASIED
                     FPDDRWDAIIAINLSAVFHGMKAAIPGMKRKGWGRIINIASAHGLVASGQKSAYVAAK
                     HGVVGLTKVAAIELANSGVTANAICPGWVLTPLVQRQLDARAKAEGISAEQAAQAFLS
                     EKQPMTRFSTPEQIGGLAVFLCSPAADTITGVPLSIDGGWVAQ"
     gene            1151..2332
                     /locus_tag="VFN42_11515"
     CDS             1151..2332
                     /locus_tag="VFN42_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011252922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET8997285.1"
                     /translation="MADVVTRRAAIMGGAALIPSVAFGQNVRPQLATPPSVISNPPRQ
                     WGRYAPPDIYPDPDILVIDNSFKRYVVGLAGIHRLWTGFQWAEGPAWSGEGQYVVFSD
                     VKGDTQYRYIWESGRVTTYRKPSYNSNGNSFDFQGRQLSTQDFFRRVVRWEPDGSMTV
                     LAAAFDGKPLNSPNDLVPHPDGSIWFTDPPYGGQLSEGHPDVKGGRTNPDGVYNPNLG
                     DDGVGLIGGVRQVLPTNTYRLDPKGGLEVVVPGSEVPDPNGLCFSPDYRTLYVISTGP
                     GPGETTGGKGVVWAFDVQGSKLGPKRLFTDCMVDGVHCGPDGMRADRAGNLWIGSNAP
                     LGYAGVTVWNPAGKLLGRIRLPEVCANLCFAGPKRDFLFMTASQSIYMVRVNIQGAAP
                     G"
     gene            complement(2409..3125)
                     /locus_tag="VFN42_11520"
     CDS             complement(2409..3125)
                     /locus_tag="VFN42_11520"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8997286.1"
                     /translation="MAADALVLSDIDALYGDSHVLHRVSWRLGEGRLLGLLGRNGAGK
                     TTCMSVAVGLLPPRRGKVAINGVQVAGRSPEAIAAQGMALVPQGRRIFRSLTVRENLA
                     VAARKSRTHDSPWDFDRVYEIFPRLNERRDQYAGYLSGGEQQMLAIGRALMSNPRVLL
                     MDEPSEGLAPQIVAEVMAIIRRLKEHGLSIVLVEQNANLVLDVADDIVVLNSGRVVVE
                     DTTEALQRNGVDLHQHLGIF"
     gene            complement(3115..3852)
                     /locus_tag="VFN42_11525"
     CDS             complement(3115..3852)
                     /locus_tag="VFN42_11525"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8997287.1"
                     /translation="MTALEVRGLNKSFGGLRVTGGVDLVVQAGERRLIIGPNGAGKTT
                     LFNLITGELRADGGRVVLFGSDITRAATRRRAHMGLARTYQIITLFPSETLLHNVTLS
                     LLGLSPLRWNPILRLDRQHHLMEQALAALGRVGLQHLAERPVSQTSYGERRRVEIAMA
                     LAQAPKVLLLDEPFAGLSVEERGDVLRLIGGIPRDVTIVMIEHDMDVALEFAERITLL
                     HFGQVVVEGTRAEVVEHPRTKEIYLGG"
     gene            complement(3839..4831)
                     /locus_tag="VFN42_11530"
     CDS             complement(3839..4831)
                     /locus_tag="VFN42_11530"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8997288.1"
                     /translation="MKRIALAVALVALATLPFWIGNSFYINVSAQILIYAIFALGLNV
                     LAGYAGLVTLGHAGLFGVAAYTAGLLIAAGHGQLIVALGACAMTVVVAAIFAVLSLRG
                     TGLGFVMITVALGEICYGLAFRWISITNGDNGITINVRPEPFGLSLATPQAFYWATLV
                     VFVLTVASMAVFVASPFGAALRGTRDQPRRMTVLGYNVWLTRFLAFLFSGFWSAVAGL
                     LYLYYNQFISPEAVSLGSSAEALLMVIAGGTGTLLGPIVGAGLVVIIKDVVSAYISRW
                     NFMLGLIFVLIVVFMPDGLVPGSIRLWRMITSPRGLRRSVPTTTPAVKVADDGA"
     gene            complement(4828..5688)
                     /locus_tag="VFN42_11535"
     CDS             complement(4828..5688)
                     /locus_tag="VFN42_11535"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8997289.1"
                     /translation="MNLWVVLSINSVTFGGLLFLLSVGFALIFGLMRIPNLTHGSLFM
                     LGAYFGVTLIGWGLNFWEAALVAGLGVALFGALVERFILRRLVGAELAQVLVTLGLAF
                     MIADSCLMLWGGDPISVSTPPHAVGFTRIAGVAFPTYRLVIILIAAAIAAALWAMIER
                     TRLGARIRAAVDDPAMARVCGIRVSSLFTIVFALGAGLAGFAGVIGGPILSVYPGLDQ
                     DMLPLALVVVILGGTGRLLGSLVGSFVVAILYNFGQALFPELAYVILFLPMLIILVLR
                     PQGLFGRVVL"
     gene            complement(5713..6939)
                     /locus_tag="VFN42_11540"
     CDS             complement(5713..6939)
                     /locus_tag="VFN42_11540"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8997290.1"
                     /translation="MTFGLNRRTFLAASAAASLPMPAIAQSKPVRIGLLTVKSGPLAQ
                     GGLQMIQGVELFIKTRNKMIAGRPIELIIGDTGGNPAGTKSKAQELIERDHVDMIFGP
                     LAAFELLAISNYVAEHKTPILSLAAAEDMTQRKPNPYFVRASGTSAQPMHPLADFARK
                     ELHYKTAITISEDFAFGYEQMGGFQRVFDDEGGRVVNKLWPPIVTPDYTPYLAQMSGV
                     DVIVQGFAGSNPLKFMKQYHDAGLKIPVLGGGPAGDDALMKSFGDEALGMVSASAYTS
                     DLDTPSNKKFVAGMIKETGSIPGLYAAGMYINGLIAEAALKKLGGKTDDKKALITAMR
                     AVSLEDSPRGPFHFDHFGNVVGNIFIRRVVKQNGKLAQHTIKTYHNVSQFWTFKEKWF
                     LEQPVYSRNYPPLLKS"
     gene            complement(7096..7476)
                     /gene="hpxZ"
                     /locus_tag="VFN42_11545"
     CDS             complement(7096..7476)
                     /gene="hpxZ"
                     /locus_tag="VFN42_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalurate catabolism protein HpxZ"
                     /protein_id="HET8997291.1"
                     /translation="MQINLPDVLVEVEAAFARYEDALMRNDTDALQALFWNSPYTIRY
                     GIGENLYGHAEIGAFRAARSPLGLARSLSRTVITTFGRDFATASTLFHRDSAPGKVGR
                     QMQTWTRMPDGWRIVAAHVSMIDG"
     gene            7672..8820
                     /locus_tag="VFN42_11550"
     CDS             7672..8820
                     /locus_tag="VFN42_11550"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET8997292.1"
                     /translation="MNAPVAVDYGPEQAAMQAYLQEGERRAFSLGNRGPIRFTAEGKL
                     HPDILDAYWRCGFYVFEGVLPAEELADIEADIKTNVLARLPVEKGAAVDAQGLPALGS
                     GLQAPVLFWSKPLGDPFGGTSLAGGRHPVKMFEPQAAKDAPKEIVYLILGSLQFSEAH
                     LRLYGHPHLLAVAAAVNGDDFVPFNEALFIKEPGRGASVAWHQDGLTHWDSPDWDEGS
                     HGFNFMAQLYGCTPANGVWVLPGSHKRGKADIKAMVAAAGTERLPDAVPIVCKPGDVA
                     ISNRQSVHGSFANTSKDWRITLNFGFHRRRSVLGVQGGGVHNAAAVYDAARIAQRARL
                     IGYGIDARRQRYPAETPYAYAPHAGQTWRWDAAAKQAIRDYNLLDLSI"
     gene            9067..9756
                     /locus_tag="VFN42_11555"
     CDS             9067..9756
                     /locus_tag="VFN42_11555"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HET8997293.1"
                     /translation="MQPSTMMRDRRTAHASRCRPRRASWWRGGVVLVLLACLTACAMT
                     PQPAVLSADDRTALTQVSAYLNNLQRFQATFTQSGPQGVERGEVWLDRPGRMRVEYTA
                     PGPETILANHGQLLVVNPSTGATTTMPVSRTPLDILLRSEIPLSGPVTVTALRREPGL
                     LSVSLAKTDAPRQGTLTLRFSANPLALRGVIIQDRSGRVTALALQDIVRNPRFAPDQF
                     IASPGTAAGPG"
BASE COUNT         1710 a         3299 c         3318 g         1585 t
ORIGIN      
        1 gaatcgcggg ctggtgcgca ccagcaaccg ggtggtgaag caggacggca agctggcgct
       61 gacctatacg ccgctgcgca tggtgaaggc gcgcagcctc ggttgagtcc gacgcctccg
      121 tgtcatgcac gggcttggcc cgtgcaccca cgtcttgctc ggtatgagtc ggactttccc
      181 catcccgccg gaccttctac gctgctgcac cgcaacaccc cggagtttgc cccatgtccc
      241 tcagcggcaa gaccgcgctc gtcaccggtt ccaccagcgg catcggcctg ggcatcgccc
      301 gcgtgctggc agggcagggg gccaacctga tgctgaacgg cttcggtgat gcggcggaga
      361 tcgaaaccct gcgtgccgaa ctggagcggg agcacaagat ccgcgtcgcc tatcatcccg
      421 ccgacatgag caagccggcg cagatcgccg cgatggtcgc ggtggccgcg gagcagttgg
      481 gtggcgtgga catcctggtg aacaacgccg gcatccagca cgtggccagc atcgaggatt
      541 tcccggacga ccgctgggac gcgatcatcg ccatcaacct gtccgcggtg ttccacggca
      601 tgaaggcggc gatccccggc atgaagcgca aaggctgggg ccgcatcatc aacatcgcct
      661 cggcgcacgg cctggtggcg agcgggcaga aatccgccta tgtcgccgcc aagcacggcg
      721 tggtggggct gaccaaggtg gccgcgatcg agctggccaa cagcggtgtc accgcgaatg
      781 cgatctgccc cggctgggtg ctgaccccgc tggtgcagcg gcaattggac gcgcgcgcca
      841 aggccgaggg catcagtgcg gagcaggcgg cgcaggcctt cctgtcggag aagcagccga
      901 tgacgcggtt ttccacgccg gagcagatcg gcgggctggc ggtgttcctg tgctcgcccg
      961 cggcggacac gatcaccggc gtgcccttgt cgatcgacgg cggctgggtg gcgcagtagc
     1021 gcgcctgccg ggctgcccgt ccgccgaatc cgcctctgcc acacgatgcg gtgaagcggg
     1081 cgggaggtga ttgacagtca ccgccaatgc tccgccaatc tcgcccaggg aaaacgggtg
     1141 gagacgggac atggccgatg tcgtgacacg cagagcggca atcatgggcg gtgctgcact
     1201 gattcccagt gtcgcttttg gccagaatgt caggccgcag cttgcgacgc cgcccagcgt
     1261 gatcagcaac ccgccgcgcc aatgggggcg gtacgcgccg ccggatatct atcccgaccc
     1321 cgacatcctg gtgatcgaca acagtttcaa gcgctacgtc gtcgggctgg caggcatcca
     1381 tcgcctgtgg accgggttcc aatgggccga aggcccggcc tggtcgggcg aaggccagta
     1441 cgtcgtgttc agcgacgtga agggcgacac gcagtatcgc tatatctggg aaagcgggcg
     1501 ggtcaccacg tatcggaagc cgtcctacaa cagcaatggc aattccttcg atttccaggg
     1561 ccgccagctc tctacgcagg atttcttccg acgcgtggtg cgctgggagc cggatggctc
     1621 gatgacggtg cttgccgcgg cattcgatgg caagccgctg aactcaccca atgatctggt
     1681 gccgcatccg gatggcagta tctggttcac cgatccacct tatggcgggc aattgagtga
     1741 aggccatccc gacgtgaaag gtggccgcac caatccggac ggcgtctaca atcccaatct
     1801 tggcgacgac ggcgtcggct tgatcggggg cgtgcggcag gtgctgccga ccaacaccta
     1861 tcgcctggac ccgaaaggcg gactggaggt cgtcgtgccc ggcagcgagg tgccggatcc
     1921 gaacggtctg tgcttctcgc cggattaccg gacgctgtat gtgatcagca ccggtccggg
     1981 cccgggcgag acgacgggcg gcaaaggcgt ggtctgggcg ttcgacgtgc agggcagcaa
     2041 gctgggaccc aagcgcctgt tcaccgactg catggtggac ggcgtgcatt gcggcccgga
     2101 cggcatgcgc gccgaccggg ccggcaatct gtggatcggt tccaacgcgc cgctcggcta
     2161 tgccggcgtc acggtgtgga atccggcggg caagttgctc ggccgcatcc gcctgccgga
     2221 agtgtgcgcc aatctgtgct ttgccggccc caagcgcgat ttcctgttca tgaccgcgag
     2281 ccagtcgatc tacatggtgc gcgtcaacat ccagggtgcc gcccccggct gagcggcggg
     2341 cgaggctgcg cccgcaagcg cgcaccacgt cgtggcgggc ctgcgcccgc aacgacgtta
     2401 tcagccgctc agaaaatccc cagatgctgg tgcagatcca cgccgttgcg ctgcagcgcc
     2461 tcggtggtgt cttccaccac cacgcggccg ctgttcagca ccacgatgtc gtccgcgacg
     2521 tccagcacca ggttggcgtt ctgctccacc agcacgatcg acagcccgtg ttccttcagc
     2581 cggcggatga tcgccatcac ctccgcgacg atctgcggcg ccagcccctc cgacggttcg
     2641 tccatcagca gcacgcgcgg gttggacatc agcgcgcggc cgatcgccag catctgctgc
     2701 tcgccgccgg acagatagcc ggcgtactga tcgcgccgtt cgttcagccg cgggaagatc
     2761 tcgtacaccc ggtcgaaatc ccacggcgaa tcgtgcgtgc gcgacttgcg cgccgccacc
     2821 gccaggttct cccgcaccgt caggctgcgg aagatgcggc gtccctgcgg caccagcgcc
     2881 atgccctgcg cggcgatcgc ctcgggcgag cggcccgcca cctggacgcc gttgatcgcc
     2941 accttgccgc ggcgcggcgg cagcaagccc accgcgacgc tcatgcaggt ggtcttgccc
     3001 gcgccgttgc ggcccagcaa tcccagcagc cggccttccc ccagccgcca ggacacgcgg
     3061 tgcagcacgt ggctgtcgcc gtacagcgcg tcgatatcgc tcagcaccag ggcatcagcc
     3121 gccaaggtag atctccttcg tgcgcggatg ctccaccact tcggcgcgcg tgccttccac
     3181 caccacctgg ccgaaatgca gcagcgtgat gcgttcggcg aattccagcg ccacgtccat
     3241 gtcgtgctcg atcatcacga tcgtcacgtc gcgcggaatg ccgccgatca gccgcagcac
     3301 gtcgccgcgt tcttccaccg ataagccggc aaacggctcg tccagcagca gcaccttggg
     3361 tgcctgcgcc agcgccatgg cgatctccac ccgccgccgc tcgccgtagg aggtctgcga
     3421 gaccggccgt tccgccagat gctgcagccc cacgcggccc agcgccgcca gcgcctgctc
     3481 catcaggtgg tgttgccggt ccagccgcag gatcggattc cagcgcagcg gcgacaggcc
     3541 gagcagcgac agcgtcacgt tgtgcagcag cgtttcgctg gggaacaggg tgatgatctg
     3601 ataggtgcgc gccaggccca tatgcgcgcg ccggcgcgtc gccgcgcggg tgatgtcgct
     3661 gccgaacagc accacccgcc cgccatcggc acgcaattcg ccggtgatca ggttgaacag
     3721 cgtggtcttg ccggcgccgt tcgggccgat gatcagccgg cgctcgcccg cttgcaccac
     3781 caggtcaacg ccgccggtga cgcgcaatcc gccgaaggat ttgttcagcc cgcgaacttc
     3841 aagcgccgtc atcggccacc ttcaccgcgg gggttgttgt gggaaccgag cggcggaggc
     3901 cgcggggcga tgtgatcatg cgccacagcc ggatgctgcc cggcaccagg ccgtccggca
     3961 tgaacaccac gatcagcacg aagatcagcc ccagcatgaa gttccagcgg gagatgtacg
     4021 cgctgaccac gtccttgatg atcaccacca ggccggcccc gacgatcgga cccagcagag
     4081 tgccggtgcc gccggcaatc accatcagca gcgcctcggc cgaggaaccc agcgacacgg
     4141 cttccgggct gatgaactgg ttgtagtaga ggtacagcag ccccgcgacc gcgctccaga
     4201 agccggagaa caggaaggcc aggaagcgcg tcagccacac gttgtagccc agcacggtca
     4261 tgcggcgcgg ctggtcgcgc gtgccgcgca atgccgcgcc gaagggcgag gcgacgaaca
     4321 ccgccatgct ggcaaccgtc agcacgaaca ccaccagcgt tgcccagtag aatgcctgcg
     4381 gcgtggccag cgacaggccg aacggctccg gccgcacgtt gatggtgatg ccgttgtcgc
     4441 cattggtgat gctgatccag cggaacgcca ggccgtagca gatttcgccc agcgccacgg
     4501 tgatcatcac gaagcccagg ccggtgccgc gcaacgacag cacggcgaag atcgccgcga
     4561 cgaccacggt catggcgcag gcgccgagtg ccacgatcag ttggccgtgg ccggccgcga
     4621 tcagcaggcc ggcggtgtag gcggcgacgc cgaacagccc ggcatggccc agcgtgacca
     4681 ggccggcata ccccgccagc acattcaggc cgagtgcgaa gatggcgtag atcaggatct
     4741 gcgcggagac gttgatgtag aagctgttgc cgatccagaa cggcaacgtc gcgagcgcaa
     4801 ccagggcgac cgccagggcg atgcgcttca cagcaccacc ctgccgaaca ggccttgcgg
     4861 gcgtagcacc aggatgatca gcatcggcag gaacaggatg acataggcca gttcagggaa
     4921 cagcgcctgg ccgaaattgt acaggatcgc cacgacaaag ctgccgacca gcgagcccag
     4981 cagccgcccg gtgccgccca ggatcaccac gaccagtgcc aatggcagca tgtcctggtc
     5041 caggcccgga tataccgaca ggatcggtcc gccgatcacg ccggcgaatc ccgccagtcc
     5101 ggcacccagc gcaaagacga tggtgaacag tgatgacacc cggatgccgc acacccgcgc
     5161 cattgccgga tcgtccaccg cggcgcggat gcgggcgccc agccgggtcc gctcgatcat
     5221 cgcccaaagg gcggcggcga tggcggcggc gatcaggatg atcaccaggc ggtaggtggg
     5281 gaatgccacg ccggcgatgc gggtgaatcc cactgcgtgc ggcggcgtgg agacggagat
     5341 cggatcgccg ccccataaca tcaggcagga atcggcgatc atgaacgcca ggccgagcgt
     5401 gaccagcacc tgcgccagct cggctccgac cagccggcgc aggatgaagc gttcaaccag
     5461 cgcgccgaac agggcgacgc caagcccggc caccagcgcg gcttcccaga agttgagccc
     5521 ccagccgatc agggtgacgc cgaaatacgc gcccagcatg aacagggagc cgtgcgtcag
     5581 gttgggtatg cgcatcaatc cgaagatcag tgcgaaccca accgacagca ggaacagcaa
     5641 accgccgaac gtcacgctgt tgatggagag aacgacccag aggttcacag cgtgtcgccc
     5701 cttgaccgaa ggtcagctct tcagcagggg cgggtagttg cgggaataca cgggttgctc
     5761 caggaaccat ttttccttga aggtccagaa ctggctgacg ttgtggtagg tcttgatggt
     5821 gtgctgcgcc agcttgccgt tctgcttcac cacgcggcga atgaagatgt tgcccaccac
     5881 attgccgaaa tggtcgaagt ggaacggccc gcgcggtgaa tcttccagcg acaccgcgcg
     5941 catcgccgtg atcagcgcct tcttgtcgtc ggtcttgccg cccagcttct tcaacgcggc
     6001 ctcggcgatc aggccgttga tgtacatgcc tgcggcatac aggccgggga tcgacccggt
     6061 ttccttgatc atgccggcga cgaacttctt gttgctcggc gtatccaggt cggaggtgta
     6121 ggcggaggca ctgaccatgc cgagggcctc gtcgccgaag ctcttcatca gcgcgtcgtc
     6181 gccggccgga ccgccgccca gcaccgggat cttcaatccg gcatcgtggt actgcttcat
     6241 gaatttcagc ggattcgaac cggcgaagcc ctgcacgatg acatccacgc cgctcatctg
     6301 cgccaggtac ggggtgtagt ccggtgtgac gatcgggggc cacagcttgt tgacgacgcg
     6361 gccaccctcg tcatcgaaca cgcgctggaa accgcccatc tgctcgtagc cgaaggcgaa
     6421 gtcctcagag atggtgatgg cggtcttgta atgcagctcc ttgcgggcga agtcggccag
     6481 cgggtgcatg ggctgcgccg aggtgccaga cgcgcgcacg aagtaggggt tcggcttgcg
     6541 ttgcgtcatg tcctcggcgg cggccaggct gaggattggc gtcttgtgct ctgccacata
     6601 attggagatc gccagcagtt cgaaggcggc gagcggaccg aagatcatgt cgacgtggtc
     6661 gcgctcgatc agttcctggg ccttgctctt ggttccggcc gggttgccgc cggtatcgcc
     6721 gatgatcagc tcgatcggcc ggccggcgat catcttgttc cgggttttga tgaaaagctc
     6781 gacgccctgg atcatctgca gcccaccctg cgccagggga ccgctcttca ctgtcaacag
     6841 gccgattcga accggcttgc tttgcgcgat tgccggcatc ggcagggatg cggcggcgct
     6901 ggcagccagg aacgtgcgcc gattcaaacc aaaggtcata atctggtttc ctcccttccg
     6961 gtctattccg gattgccacc ttggtagccg acaacagatc ggcttggaaa gccccccaac
     7021 cgggtgcccc gcgaagttga tacgattccg gtctaatacc gcttgcccga tttgccggcc
     7081 gtccggccgc ccggatcagc cgtcaatcat gctgacatgc gccgcgacaa tccgccaacc
     7141 atccggcatg cgggtccacg tctgcatctg ccgtccgacc ttgcccggcg cgctgtcgcg
     7201 atggaacagc gtggacgcgg tggcgaagtc gcggccaaaa gtggtgatca ccgtgcgcga
     7261 cagcgatcgt gccaggccga gcggcgatcg cgcggcacgg aacgcgccga tttcggcatg
     7321 gccatacagg ttctcgccga tgccatagcg gatggtgtac gggctgttcc agaacagcgc
     7381 ctggagcgca tcggtgtcgt tgcgcatcag cgcatcctca tagcgggcga aggcggcttc
     7441 gacctccacc agcacgtcgg gcaggttgat ctgcatgggc cactccatcg gttgctgtcg
     7501 cgcggggatc atgggtgaag ccgcgggcgg tgtctcccct tcagcctggc agtctgcctg
     7561 actgacacgg caaggccggc gctgccgggt gggccccgcc ggtgcccggc ggaattgtca
     7621 gctccggcgg agctggttaa ggcttgccga cacaggtgaa ggagacaggc gatgaacgct
     7681 ccggtcgcgg tcgattacgg tcccgagcaa gccgccatgc aggcctatct gcaggagggc
     7741 gagcggcgcg ctttcagcct gggcaatcgc ggcccgatcc gcttcaccgc cgagggcaag
     7801 ctgcatcccg atatcctgga cgcctattgg cgctgcggat tctacgtgtt cgagggcgtg
     7861 ctgccggcgg aggaacttgc ggacatcgag gccgacatca agaccaatgt gctggcgcgc
     7921 ctgccggtgg agaagggcgc ggcggtggac gcgcaagggc tgcccgcgct tggcagcggc
     7981 ctgcaggccc cggtcctgtt ctggtccaag ccgctcggcg acccgttcgg cggcacgagt
     8041 ctggcgggcg gccgccaccc ggtgaagatg ttcgagccgc aggcggcaaa ggatgccccg
     8101 aaggagatcg tgtatctgat cctcggctcg ctgcagttct ccgaagcgca tctgcggctg
     8161 tacgggcatc cgcatctgct ggcggtcgcg gcggcggtga acggcgacga tttcgtgccg
     8221 ttcaatgagg cgctgttcat caaggaaccc ggccgcggcg cgtcggtcgc ctggcaccag
     8281 gacgggctga cgcattggga cagcccggac tgggatgaag gctcgcacgg gttcaatttc
     8341 atggcgcagc tttatggctg cacgccggcc aatggagtct gggtgctgcc cggatcgcac
     8401 aagcgcggca aggcggacat caaggcgatg gtggcggcgg cgggaaccga gcggctgccc
     8461 gatgcggtgc cgatcgtgtg caagccgggc gacgtggcga tcagcaaccg gcagtccgtg
     8521 catgggtctt tcgccaatac cagcaaggac tggcgcatca cgctgaactt cggcttccat
     8581 cgccgccggt cggtgctggg cgtgcagggc gggggcgtgc acaacgcggc ggcggtgtac
     8641 gacgcggcgc gcatcgcgca gcgcgcccgt ctgatcggct atggcatcga tgcccgacgg
     8701 cagcgttatc cggcggaaac gccctatgcg tatgcgccgc atgccgggca gacctggcgc
     8761 tgggatgcag cggcgaaaca ggcgatccgc gactacaacc tgctcgatct gagcatctga
     8821 gtcagccacc gcgtcatggc gggcgaaggc ccgccaccca tgaattgctg ggggagactc
     8881 acgagtcgca agccgctttg ggggggctcc cacagtcagt cgtgggtgcg ggccttcgcc
     8941 cgccaccgcg tagggcgacc ggtgatctcc gcccgccatg cccgtgctcg cgcgttcttg
     9001 catcggcctt tccgcgatgc aggcaatttc ggtctgcagt accgatagcc cgatggagtg
     9061 atgccgatgc agcccagcac gatgatgcgc gaccggcgta ctgcgcatgc aagccgctgc
     9121 agaccgcggc gggcgtcctg gtggcgcggc ggcgtggtgc tggtcctgct cgcctgtctc
     9181 accgcctgcg cgatgacgcc gcagccggcg gtgctgagcg ccgatgaccg cacggccctg
     9241 acgcaggtat ccgcctatct gaacaatctg caacgattcc aggcgacgtt cacccagtcc
     9301 ggaccgcagg gcgtcgaacg gggcgaggtc tggctggacc gccctggccg catgcgggtg
     9361 gagtacaccg cccccggccc ggaaaccatc ctggccaatc acggccaact gctggtggtg
     9421 aacccgtcca ccggcgcgac caccaccatg ccggtctccc ggacgccgct ggatatcctg
     9481 ttgcgcagcg agatcccgct gagcggtccg gtgacggtca ccgcgctgcg gcgcgaacca
     9541 gggctgctga gcgtcagcct tgcgaagacc gatgcgcccc gccagggtac gctgacgctg
     9601 cgcttcagcg ccaacccgct ggcgctgcgc ggggtgatca tccaggaccg ctctggccgg
     9661 gtgaccgcgc tggcgctgca ggacatcgtg cgcaatccgc gtttcgcgcc ggaccagttc
     9721 atcgccagcc ccggcacggc agcggggccg ggctgacgcc agacggcatt tgcgggagac
     9781 tgcggccatg cgtgctgtgc tgatgctgct gatcgccgcc ggccccgcgg gggcggccac
     9841 cgcgcccggt gcggacgtgc tgacctatca cggcgatccg cagcgcagcg gccactacac
     9901 ggtgcagcgc ct
//
LOCUS       DASSZH010000529         2729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_114918, whole genome shotgun sequence.
ACCESSION   DASSZH010000529 DASSZH010000000
VERSION     DASSZH010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114918"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VFN42_11560"
     CDS             complement(<1..585)
                     /locus_tag="VFN42_11560"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HET8997294.1"
                     /translation="MALTASAPVPARAQSAPECPPAPLIRLHLPHLQAALTAGKEAVI
                     VALGSSSTRGEKASDPAHSYPAVLQTRLAQALPGWHVAVLNRGINGQDAPEELARLSA
                     DVIAVRPQLVIWQVGANGAMRKSDPERFRQRVTEGVRELQAAGIDVVLMDNQHCPRVD
                     ATPEGVLLDQVLGQVAKQVGAGLFSRAALMETWQR"
     gene            complement(701..1540)
                     /locus_tag="VFN42_11565"
     CDS             complement(701..1540)
                     /locus_tag="VFN42_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HET8997295.1"
                     /translation="MRTSPFEVRPIAGALGAELHGVDLSQDLPEATVAALRQALLDHL
                     VIFFRDQDLPPDRFLALARRFGTPIEYPFVKGIAGYPEVIAVAKLPGEQINFGGVWHS
                     DTTYLTEPPMATLLVAREVPPFGGDTIFANQYLAYETLSPAMQHVLAALRGISSSRKA
                     DASRTREDRIRTDGTAEPHKALTAEHPVVRAHPETGRKALYVNVAHTVGLAGLTEEES
                     APLLAYLFRHQIRPEFTCRFRWQPGSLAIWDNRCAQHYAVNDYQGQTRVMHRITLAGD
                     RPR"
     gene            complement(1619..2494)
                     /gene="sucD"
                     /locus_tag="VFN42_11570"
     CDS             complement(1619..2494)
                     /gene="sucD"
                     /locus_tag="VFN42_11570"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002730338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HET8997296.1"
                     /translation="MAILVNRDTRVICQGFTGAQGTFHSEQAIAYGTRMVGGVTPGKG
                     GSTHLNLPVFDTVADAVTTTGANATAIYVPPPFAADAILEAVDAEIPLVVCITEGIPV
                     LDMVKVKRALSGSKSRLIGPNCPGVITPEECKIGIMPGHIHRRGKVGIVSRSGTLTYE
                     AVAQTTAENLGQSSCVGIGGDPVKGTDFIEVLEMFLGDKQTEAIIMIGEIGGESEILA
                     AEFLAREKTKKPTVGFIAGATAPPGRRMGHAGAVISGGRDTAGAKMDAMRSAGIHVAA
                     SPAELGSTLAGVLRG"
     gene            complement(2496..>2729)
                     /gene="sucC"
                     /locus_tag="VFN42_11575"
     CDS             complement(2496..>2729)
                     /gene="sucC"
                     /locus_tag="VFN42_11575"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386312.1"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HET8997297.1"
                     /translation="AILVNIFGGIMRCDVIAEGVVAAAREVSLNKPLVVRLEGTNVAK
                     GKEILAQSGLPIIAADNLADAAQKAVAAVKGAA"
BASE COUNT          432 a          927 c          913 g          457 t
ORIGIN      
        1 gcgctgccag gtttccatca gcgccgcgcg ggagaacagg ccggcgccca cctgcttggc
       61 aacctgcccg agaacctgat ccagcagcac gccttccggg gtcgcgtcca cacgcgggca
      121 atgctggttg tccatcagca ccacgtcgat cccggctgcc tgcaattccc gcacgccctc
      181 ggtgacgcgt tggcgaaacc gctcggggtc ggactttcgc atcgcgccgt tggcgccaac
      241 ctgccagatg acgagctgcg ggcgaaccgc gatcacatcg gcggagaggc gtgccagttc
      301 ctcgggggcg tcctgtccat tgatgccacg attgagcacg gcgacgtgcc atccggggag
      361 cgcctgtgcc agccgggttt gcagcacagc ggggtatgaa tgggcaggat cggaggcctt
      421 ttcaccccgg gtagaggacg aacccagggc gacgatcacc gcttccttgc cggcggtcag
      481 cgcggcctgc aaatgcggca gatgcaggcg gataagcggc gcgggcggac actccggagc
      541 ggactgcgcc cgggcaggca ctggcgcgga cgcagtcagc gccaccgcgc aaagcaggca
      601 tcccaggcgt gatatgaacg aggcccccaa cattatccca gtcaaaccga caggccgtga
      661 tttgtccaga gtcaaaaatg gtgaggacgt gaaattccag tcaacggggt cgatcgcccg
      721 ccagcgtgat ccggtgcatc acccgcgtct gcccctggta gtcgttcacc gcataatgct
      781 gcgcgcagcg gttgtcccag atcgccagtg agccgggctg ccagcggaac cggcaggtga
      841 actccgggcg gatttggtga cggaacaggt acgccagcag cggggcgctt tcctcctcgg
      901 tcagcccggc aagaccgacc gtgtgcgcca cattgacata cagcgccttg cgcccggtct
      961 ccggatgcgc gcgcaccacc ggatgctccg ccgtcaatgc cttgtgcggc tccgcggtgc
     1021 cgtcggtccg gatgcggtct tcccgcgtcc ggctggcgtc cgccttcctg gaactggata
     1081 tcccgcgcaa cgctgccagc acgtgctgca tggcgggcga cagggtttcg tacgcgaggt
     1141 actggttggc gaagatggtg tcgccgccaa acggcggcac ctcgcgcgcg accagcagcg
     1201 tggccatggg cggctcggtc agataggtcg tgtcggaatg ccagacgccg ccgaagttga
     1261 tctgctcgcc cggcagcttg gccacggcga tgacttcggg atagccggcg atacccttga
     1321 cgaacgggta ttcgattggc gtgccgaagc ggcgggccag ggcgaggaag cgatccggcg
     1381 gcagatcctg atcgcggaaa aagatcacca ggtggtccag cagcgcctgc cgcagtgccg
     1441 cgacggtggc ctcgggcagg tcctgcgaca ggtcaacgcc gtgcagttcg gcgcccaggg
     1501 cgccggcgat cggacgaacc tcgaacgggc tagtacgcat gatcctgctg ctcctgcggt
     1561 cgccggagca gcgtgaccgg aaatgcgccg gggaccaagc cccggcgcat tgctgggttc
     1621 agccgcgcag cacgccggcc agggtgctgc ccagctcggc cgggcttgcc gcgacatgga
     1681 tgccggcgct gcgcatcgcg tccatcttgg cgccggcggt atcgcgaccg ccggagatca
     1741 ccgcaccggc atggcccatg cggcggccgg gcggcgcggt ggccccggca atgaagccca
     1801 ccgtcggctt tttggtcttc tcgcgggcca ggaattccgc cgcgaggatt tcgctctcgc
     1861 cgccgatctc gccgatcatg atgatcgctt cggtctgctt gtcgccgagg aacatctcca
     1921 gcacctcgat gaagtcggtg cccttcaccg ggtcgccacc gataccgacg caggaactct
     1981 ggccgaggtt ttccgccgtg gtctgcgcca ctgcttcata ggtcagcgta ccagagcggc
     2041 tgacgatgcc caccttgccg cggcgatgga tgtgcccggg catgatgccg atcttgcact
     2101 cttccggcgt gatcacgccc gggcagttcg gcccgatcag gcgcgacttg ctgccgctga
     2161 gcgcgcgctt caccttcacc atgtccagca cgggaatgcc ctcggtgatg cacaccacca
     2221 gcgggatttc cgcgtccacc gcctccagga tcgcgtcggc ggcgaagggc ggcggcacgt
     2281 agatcgcggt ggcgttcgcg cccgtggtcg tcaccgcatc ggcgacggtg tcgaacaccg
     2341 gcaggttcag atgcgtgctg ccgcccttgc ctggcgtcac gccgcccacc atgcgcgtgc
     2401 cgtaagcgat cgcctgctcg ctgtggaatg tgccctgggc gccggtgaag ccttggcaga
     2461 tcacccgcgt gtcgcggttc accagaatgg ccatgtcacg ctgctccctt cactgcggca
     2521 acggctttct gcgccgcgtc cgccaggttg tcggctgcga tgatcggcag gccggactgt
     2581 gcgaggatct ccttgccctt cgccacgttg gtgccttcca ggcgcaccac cagcggcttg
     2641 ttcaggctga cctcgcgcgc cgcggcgacc acgccctcgg cgatcacgtc gcagcgcatg
     2701 atgccgccga agatgttcac caggatcgc
//
LOCUS       DASSZH010000530         4336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_148968, whole genome shotgun sequence.
ACCESSION   DASSZH010000530 DASSZH010000000
VERSION     DASSZH010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148968"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /gene="hflX"
                     /locus_tag="VFN42_11580"
     CDS             complement(<1..993)
                     /gene="hflX"
                     /locus_tag="VFN42_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET8997298.1"
                     /translation="MRAADARLAEAVGLTASIGLVVVHTAILPLRVRRPSTLLGEGQV
                     QQQATAIREHDVTVVIVDAALSPVQQRNLERAWSCKVIDRTGLILDIFGERAATKEGS
                     LQVELAHLEYQRSRLVRSWTHLERQRGGFGFLGGPGETQIEADRRLIGDRIVRLKKEL
                     EQVRRTRGLHRSARRRVPFPVVALVGYTTAGKSTLFNALTGAEVTARDQLFATLDPTM
                     RGLRLPSGRRVILSDTVGFISELPHELVEAFRATLDEVKEADIILHVRDIASAESEAE
                     AGDVRRVLDELGAGDEAGQRVLEAWNKIDLLAPDAREFLTARAARTDVGAVAVSA"
     gene            complement(1151..1441)
                     /gene="hfq"
                     /locus_tag="VFN42_11585"
     CDS             complement(1151..1441)
                     /gene="hfq"
                     /locus_tag="VFN42_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HET8997299.1"
                     /translation="MANEKSQNVQDVFLNHVRKNKTPVTVFLVNGVKLQGIITWFDNF
                     SVLLRRDGHTQLVYKHAISTVMPGAPIQLFDATKAEASAAPARELATAGTGH"
     gene            complement(1539..2186)
                     /locus_tag="VFN42_11590"
     CDS             complement(1539..2186)
                     /locus_tag="VFN42_11590"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET8997300.1"
                     /translation="MTAPRPTVLLYDWDNTLVDGWAGITAALNAVFAAFDRPLWSVQD
                     TRERVRVSLRESFPIMFGDQWEHARDLFYDTLAEQHLQHVQPMPGAAEALLAGAPWPQ
                     GVVSNKAGRYLRAEVAHLGWAGHFGPVIGAGDASADKPDPAPIHLALSRLARIADESV
                     WYLGDTALDMQAARAAGVTAVLIGDAGHDGGVAHAAPHLHFSSALHLAARLRELM"
     gene            2356..2664
                     /locus_tag="VFN42_11595"
     CDS             2356..2664
                     /locus_tag="VFN42_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HET8997301.1"
                     /translation="MRLLRLLISLLILLVLVLFALSNTAPVQIGIWPTGLSLHAPLSL
                     VVLGGMAIAFLLGGMLVWFTELSQRRRARRAERTIRMLEAQVQELKARVPQPMLAPPV
                     "
     gene            2661..3353
                     /gene="pyrF"
                     /locus_tag="VFN42_11600"
     CDS             2661..3353
                     /gene="pyrF"
                     /locus_tag="VFN42_11600"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET8997302.1"
                     /translation="MSARLIVALDTTDPRRAAGWAAATAPHAGLFKLGLEFFLANGPD
                     GVRATAGRPIFLDLKLHDIPNTVAGAVRAVLPLKPRMLTVHAAGGPAMLEAARNAAEQ
                     AGADRPVILAVTVLTSLAAADLAATGIADAPAAQVLRLARLAVAAGAQGLVCSPLEVA
                     MLRDALGSGPTLVVPGIRPAGAAQGDQARTMTPEQAVAAGADFIVVGRPITGAADPAA
                     AAAAIAAAAAIA"
     gene            3350..3982
                     /locus_tag="VFN42_11605"
     CDS             3350..3982
                     /locus_tag="VFN42_11605"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET8997303.1"
                     /translation="MSGVRVKICGINSAEACDAAVQAGADWIGFVFFPPSPRYVTAAQ
                     AAALAARHPDGPPRVGLFVDPTPEMVAAVLAEVRLDVLQLYGQMDLPALRARFGLPVW
                     RAMGIERAADLPVDAVGADALVLEAKPPPDATRPGGNATRFDWSLLRGWHAPAPWVLA
                     GGLTPDNVAEAIRATGAHAVDVSSGVERSRGVKDPALIRRFVTNARSADG"
     gene            complement(3993..>4336)
                     /locus_tag="VFN42_11610"
     CDS             complement(3993..>4336)
                     /locus_tag="VFN42_11610"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET8997304.1"
                     /translation="VVLDARNEPQPDITVVRRPWHGYPDAHPGPEDICLLIEVADTSI
                     ATDRGAKRELYARAGIPEYWLIDLTTDRVHVHRKPKNGVYATMRTVGPDALLEIEALP
                     GVTLKSAALFG"
BASE COUNT          624 a         1495 c         1546 g          671 t
ORIGIN      
        1 ggcggagacc gcaacggcgc cgacgtcggt gcgcgcggcg cgggcagtca ggaattcccg
       61 cgcatcgggc gccaggaggt cgatcttgtt ccacgcttcc agcacgcgct ggccggcctc
      121 gtcgccggcg cccagctcgt cgagcacgcg gcgcacgtcg ccggcctcgg cctcgctctc
      181 ggcgctagcg atgtcgcgca cgtgcaggat gatgtcggcc tccttcacct cgtcgagcgt
      241 ggcgcggaac gcctccacca gctcgtgcgg cagttcgctg atgaagccca cggtgtcgga
      301 caggatcacc cgccgaccgg agggcaggcg cagcccgcgc atcgtcgggt ccagcgtggc
      361 gaagagctgg tcgcgtgcgg ttacctcggc gccggtcagg gcattgaaca gggtggattt
      421 gccggcagtg gtgtagccga ccagcgccac caccgggaac ggcacccgcc ggcgggcgga
      481 ccggtgcagc ccgcgggtgc ggcgcacctg ctcgagctcc ttcttcagcc gcacgatgcg
      541 gtcgccgatc aggcgccggt cggcctcgat ctgcgtctcg cccgggccgc ccaggaagcc
      601 gaagccgccg cgctggcgct ccaggtgggt ccaggaccga acgaggcggg agcgctgata
      661 ttctagatgc gccagttcca cctgcagcga gccttccttg gtggcggcgc gttcaccgaa
      721 gatgtccagg atcagcccgg tccggtcgat caccttgcag gaccaggcgc gctccaggtt
      781 gcgctgctgc accggcgaca gcgcggcatc gacgatcacc acggtgacgt cgtgctcgcg
      841 gatggcggtg gcctgctgct gtacctggcc ctcgcccagc agggtggagg gccggcggac
      901 gcgcagcggc aggatggcgg tgtgcaccac caccaggccg atcgaagcgg tcagccccac
      961 cgcctcggcc aggcgcgcat cggcggcacg catgtccagc cgggtgggat cgccggaccg
     1021 gacgtgcgca tcgccattgc cgctctccgc gcgggagccg gccggacgcg tccagggcag
     1081 aatgacggcg gcccgggtgg ggccggcgtt caccgctgaa ccggacgcgc tgcgtggctg
     1141 gaagctggga tcagtgaccg gtgccggcag tggccaactc ccgtgccggg gcggcggagg
     1201 cctcggcctt ggtggcgtcg aacaactgga tcggcgcgcc cggcatcacc gtactgatgg
     1261 catgcttgta gacgagctgg gtatgcccgt cgcggcgcag cagaacggaa aaattgtcaa
     1321 accaggtgat tatcccctgc agcttgacac cgttcaccag gaacaccgtc accggcgttt
     1381 tgttcttgcg cacatggttc aggaacacgt cctgcacgtt ctgcgacttc tcgttggcca
     1441 cggatgattt ccttcttttt gctcgtgtta tccaaacagt tccggcgggt cgtggcacgg
     1501 ccgccgggag ttacgtccag tctagcagcg cggtaccctc acataagctc acgaagccgt
     1561 gcagccagat gcagcgcgga agaaaagtgg agatgcgggg cggcatgcgc aacgcccccg
     1621 tcgtgtccgg catcaccaat taataccgcc gtcacgccgg ccgctcgggc tgcctgcatg
     1681 tccagcgcgg tatccccaag ataccacacc gattcgtcag cgatacgggc aagccgggac
     1741 agcgccagat ggatcggggc tgggtccggc ttgtcggcgc tggcatcgcc ggcgccgatc
     1801 accggaccga aatgaccggc ccagcccaga tgtgccactt cggcgcgcag gtagcgaccc
     1861 gccttgttgg acaccacccc ctgcggccag ggtgccccag ccagcagtgc ctccgccgcg
     1921 ccgggcatcg gctgcacgtg ctggaggtgc tgctccgcca gcgtatcata gaacagatcg
     1981 cgcgcatgct cccactggtc gccgaacatg atcgggaagc tctcccgcag ggagacgcgg
     2041 acgcgctcgc gcgtgtcctg caccgaccat agcggccggt cgaacgccgc gaacacggca
     2101 ttgagcgcgg ctgtgatacc ggcccagccg tccaccagcg tgttgtccca gtcatacagc
     2161 aggacggtcg ggcggggtgc ggtcatggtg agcaggcggc tatgccggaa tgaaacggcg
     2221 gggacaagcg caacccccgc acggctccca tacgcaagcg ccgtaccaag ccgcggcctg
     2281 ccgccgccca cttccaaccc aggccgcttt gtggtctgtc tgcccgcact cgagactcac
     2341 ctgagcgacc ctatcatgcg cctgctgcgc ctgctcatca gcctgttgat cctgctcgtc
     2401 ctggtgctgt tcgcgctgtc gaacaccgcc ccggtgcaga tcggcatctg gcccactggc
     2461 ctgtcgctgc acgcgccact gtcgctggtc gtgctgggcg gcatggcgat cgccttcctg
     2521 ctcggcggca tgctggtgtg gttcaccgaa ctcagccagc gccgccgcgc ccgccgtgcc
     2581 gagcgcacga tccgcatgct ggaggcgcag gtgcaggagc tgaaggcgcg ggtgccacaa
     2641 cctatgctgg cgccgccggt atgagcgcgc ggctgattgt cgccctggat accaccgatc
     2701 cgcggcgtgc cgccggctgg gccgccgcca cggcaccgca tgccgggctg ttcaagctgg
     2761 gactggagtt cttcctggcg aacgggccgg acggggtgcg ggcgactgcc gggcggccga
     2821 tcttcctgga cctgaagctg cacgacatcc cgaataccgt cgcgggcgcg gtgcgcgcgg
     2881 tgctgccgct gaagccgcgc atgctgaccg tgcatgccgc cggcgggccg gcgatgctgg
     2941 aagcggcgcg aaatgcggcg gagcaggcag gcgcggaccg gccggtcatc ctcgccgtga
     3001 ccgtgctgac cagcctggcc gccgccgacc tggcggcgac gggcatcgcc gatgcaccgg
     3061 ccgcccaggt gcttcgcttg gctcggctgg ccgttgccgc cggcgcgcag gggctggtgt
     3121 gcagtccgct ggaggtggcg atgctgcgcg atgcgctcgg cagcggtccc accctggtgg
     3181 tacccggcat ccgtcccgcg ggtgcggcac agggcgacca ggcccgcacc atgacgcccg
     3241 aacaggcagt cgccgccggc gccgacttca tcgtggtggg gcggccgatc accggggcag
     3301 ccgatccggc cgccgccgcc gctgcgatcg ccgccgccgc cgccatcgca tgagcggggt
     3361 gcgggtcaag atctgcggca tcaacagcgc ggaggcctgc gacgcggcgg tgcaggccgg
     3421 cgccgactgg atcgggttcg tgttcttccc gccctcgccg cgctacgtca ctgcggcgca
     3481 ggccgcggcg ctggccgccc ggcatccgga tgggccgccg cgtgtcggcc tgttcgtcga
     3541 ccccacgccg gagatggtgg ccgcggtatt ggcggaggtc cggctggacg tgctgcagct
     3601 ctatggccag atggacctgc cggcattgcg ggcgcggttc gggctgccgg tctggcgggc
     3661 gatggggatc gagcgcgcgg ctgacctgcc agtcgatgcc gtcggcgcgg acgcgctggt
     3721 gctggaggcg aagccgccgc cggatgccac ccggccaggc ggcaatgcca cgcggttcga
     3781 ctggtcgctg ttgcgcggct ggcacgcgcc ggcaccctgg gtgctggcgg gcgggctgac
     3841 gccggacaac gtcgccgagg cgatccgcgc gacgggtgcg cacgcggtgg acgtgtcgtc
     3901 gggggtggag cggtcacgcg gcgtgaagga tccggcgctg atccggcgct tcgtgacaaa
     3961 tgcacggagc gcggacggat gagccgcacc ggtcagccga agagcgccgc ggacttcagg
     4021 gtcacaccgg gaagggcttc tatctccaga agcgcgtccg gtccgaccgt tctcatcgtg
     4081 gcatacacgc catttttcgg cttgcggtgc acgtgcacgc ggtccgtcgt cagatcaatc
     4141 agccagtact ccggaattcc ggccctcgcg tagagttcgc gcttggcgcc gcggtctgtg
     4201 gcgatgctcg tgtcggccac ctcgatcagc aggcagatgt cttcaggacc gggatgtgca
     4261 tccgggtagc catgccaggg ccgcctgacc acggtgatat ccggctgcgg ctcattgcgg
     4321 gcgtcgagca cgaccg
//
LOCUS       DASSZH010000531         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1086418, whole genome shotgun sequence.
ACCESSION   DASSZH010000531 DASSZH010000000
VERSION     DASSZH010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086418"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFN42_11615"
     CDS             complement(<1..306)
                     /locus_tag="VFN42_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997305.1"
                     /translation="MAHATTDHNQIRKWAEAKGGKPAAVDRTHKGGDVGIIRIMFPDN
                     PQSEHQSLVEISWDEFFREFESRKLALLYEDDSLFSKIVGRDTVEKREHGEHASRHHH
                     "
     gene            complement(376..1224)
                     /locus_tag="VFN42_11620"
     CDS             complement(376..1224)
                     /locus_tag="VFN42_11620"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HET8997306.1"
                     /translation="MTRLIEIRGHIASMDELLDVIGAMRSLASMRVQEAQRALPGIRR
                     YADFVAAALGAALLLRPEAEHPTRAQQGGKALVVFAGEHGFAGGFNDRLLDAAAAALA
                     PSDPLFVLGSRGAAIALQRGRQPAWHGPMATRLTSVPETLRQLEAPLYRRIVTGELAR
                     IDMLYARYRPGETAELRQEALLPLDLRALAGRPTAQPPLHNLEPERLLEGLTAEYVFA
                     LLTAAAVETMASENAARFAAMEAAHGNVETKLQRLQQEANQARQAEITEELMDLITGA
                     RTLSGG"
     gene            complement(1221..>2086)
                     /locus_tag="VFN42_11625"
     CDS             complement(1221..>2086)
                     /locus_tag="VFN42_11625"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565604.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET8997307.1"
                     /translation="VVSEGSDAPGLNFMAPYAATSIAEHFMEEGRDVLLVYDDLTQHA
                     RAYREISLLLRRPPGREAFPGDIFYIHSRLLERAAKLSPQRGGGSLTALPIAETQAGN
                     ISAYIPTNRISITDGQIYLDPRLFNEDQKPAVDVGTSVSRVGGKTQAAVLKGVAESLR
                     LEYAQFLELEVFTRFGTMTDERTRKVIEHGRRIRAVLSQPQFAPLPLGEQVALLLALS
                     AGVLDAVPLDRIAAFRQDLPDWLEQRAPEVLKLDDRAGPLPDEARDGVLRALRALAQR
                     VTGAAEAPKPS"
BASE COUNT          304 a          746 c          674 g          362 t
ORIGIN      
        1 gtggtggtgc cgggaagcgt gttcgccgtg ctcgcgcttc tccaccgtgt cccggccgac
       61 gatcttgctg aacaggctgt cgtcttcata cagcagcgcc agcttgcggg attcgaattc
      121 gcggaagaat tcgtcccagg aaatctccac caggctctgg tgttccgact gcggattgtc
      181 cgggaacatg atgcggatga tccccacatc gccgccttta tgggtgcgat ccactgcggc
      241 cggcttgccg cctttcgcct cggcccattt gcggatctga ttgtgatcgg tggttgcgtg
      301 cgccatcgaa acctcctgcc gctgttgcgt cgcacccaga acacccatcg ccagtccggg
      361 ttcggatcgc cgcattcacc cgccgctcag cgtccgcgcg ccagtgatca ggtccatcag
      421 ctcttctgtg atctccgcct ggcgcgcctg attcgcttcc tgctgcaacc gctgcaactt
      481 cgtctcgaca ttgccgtgcg cggcttccat cgcggcgaag cgggcggcgt tctcgctcgc
      541 catggtttcc accgcggccg cggtcagcag cgcgaacacg tattccgcgg tcagcccttc
      601 cagcagccgc tccggttcca ggttgtgcag cggcggctgt gccgtcggac ggcccgccag
      661 cgcccgcaaa tccagcggca acagcgcctc ctgccgcagt tccgccgtct cgcctgggcg
      721 atagcgcgcg tacagcatgt cgatccgtgc cagttcgccg gtgacgatcc ggcgatacag
      781 cggtgcctca agctggcgca gggtttccgg cacgctggtc agccgcgtcg ccatcggccc
      841 gtgccaagcc ggctgccggc cgcgctgcag cgcgatcgcg gcgccgcggc tgcccaggac
      901 gaacagcgga tcgctcggcg ccagcgcggc ggcagcggcg tccagcaggc ggtcgttgaa
      961 gccgccggcg aaaccatgct cgccggcgaa caccaccagg gccttgccgc cctgctgcgc
     1021 tcgggtggga tgctccgcct cgggccgcag cagcagcgcc gccccgagcg cggcagcgac
     1081 gaaatcggca tagcgtcgaa tccccggcaa ggcgcgttgc gcctcctgca cgcgcatgct
     1141 ggcgagcgag cgcatcgcgc cgatcacgtc cagcaattcg tccatgctgg cgatatggcc
     1201 gcggatctcg atcagccggg tcatgacggc ttgggcgcct cggccgcgcc cgtcacacgc
     1261 tgcgccagtg cgcgcaacgc ccgcagcacg ccgtcgcgcg cttcgtccgg cagcggaccc
     1321 gcgcggtcgt ccagcttcag cacctccggg gcgcgctgtt ccagccaatc cggcaggtcc
     1381 tggcggaacg ccgcgatgcg gtcgagcggc accgcgtcca gcacgccggc cgacagggcc
     1441 agcagcagcg ccacctgctc gcccagcggc aagggcgcga attgcggctg gctgagcacc
     1501 gcgcggatgc gccggccgtg ttcgatcacc ttgcgtgtcc gctcgtccgt catggtgccg
     1561 aagcgggtga acacttccag ctccaggaac tgcgcgtatt ccagccgcag gctttccgcc
     1621 acgcccttca gcaccgccgc ctgcgtcttg ccgccgacgc gggagacgct ggtgccgaca
     1681 tccaccgccg gcttctgatc ctcattgaac aggcgcgggt cgaggtagat ctgcccatcg
     1741 gtgatcgaga tccggttggt cgggatgtag gcgctgatat tgccggcctg ggtttccgcg
     1801 atcggcagcg cggtcagcga accgccgccg cgctgcggcg acagttttgc cgcgcgctcc
     1861 agcaggcgcg aatggatgta gaagatgtcg cctgggaaag cctctcgtcc cggtggacgc
     1921 cgcagcaaaa gcgagatctc ccggtaggcg cgcgcgtgct gcgtcaggtc gtcatagacc
     1981 aacaacacgt cgcgtccttc ctccatgaag tgctccgcga tgctcgttgc cgcataggga
     2041 gccatgaaat tgaggcccgg ggcgtcactt ccttcgctga ctacca
//
LOCUS       DASSZH010000532         3016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_336582, whole genome shotgun sequence.
ACCESSION   DASSZH010000532 DASSZH010000000
VERSION     DASSZH010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336582"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..599
                     /locus_tag="VFN42_11630"
     CDS             231..599
                     /locus_tag="VFN42_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET8997308.1"
                     /translation="MGTSVRSERRRRSWPEAIKREIVAASLVPGASVSVVARQYDVNA
                     NQVFAWRKLYGGTAGVPPGLRMVPVTVTPDPPAAPSPPSPWPSAGMIEIALPGGYCVR
                     VGCGVTASALRRVLDALERR"
     gene            596..943
                     /gene="tnpB"
                     /locus_tag="VFN42_11635"
     CDS             596..943
                     /gene="tnpB"
                     /locus_tag="VFN42_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183759.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HET8997309.1"
                     /translation="MIAFPASVRVWLAVGRTDMRRGMNGLALQVQQALGRDPHAGDLY
                     VFRGARGDLIKIFWHDGVGMSLYAKRLEKGRFVWPSPADGVVGISAAQLSYMLDGIDW
                     RNPRHTWRPATAG"
     gene            992..2647
                     /locus_tag="VFN42_11640"
     CDS             992..2647
                     /locus_tag="VFN42_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011427389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HET8997310.1"
                     /translation="MDTDAATPGPEEFAALQAALAAAQARADQAEAQLAAANARSSDD
                     QARIADLRLQIAKLKRQLFGTRSERTQRLLDQLELQLEELELDAGEDEIAAEQAAAKT
                     TTVAGFVRKRPSRQPLPAHLPRERVVEPAPCACPACGGTRLSKLGEDITETLEVIPRQ
                     WKVIQRVREKFSCRDCEVITQAPAPFHVTPRGWAGPNFLAMLLFEKFGQHQPLHRQAE
                     RYAREGVTLSLSTLVDQMGAGAAALLPIYRRIEAHVLAAERLHGDDTTVPVLAKGKTD
                     TGRLWVYVRDDMPFGGRAPPAAIFHYSRDRRGEHPRAHLASWSGVLQADAYGGFSDLY
                     AAGRQPGPVVEASCWAHGRRKVFELADIETAAIRQARGETPKPVYPLALEAVRRIDTL
                     FDIEREINGLPPEQRRAIRQARSAPVVAELETWMSETRDKLSRGHDLAKALNYMLRRW
                     PSFTRFLEDGRICLTNNAAERALRGVALGRKAWLFCGSDRGGQRAAILYSLIVTAKMN
                     GVDPQAWLADVLARIAGHPINRLDELLPWNWKPATSAPEAAAA"
     gene            2644..2892
                     /locus_tag="VFN42_11645"
     CDS             2644..2892
                     /locus_tag="VFN42_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997311.1"
                     /translation="MSTQRNKVHHVHTVARVARQLGVHEDLIHTLTLGLEPEDGVIWV
                     YGLDDDDGVLAFTDDGIEEVRLILAEHTEYIRRNPSKY"
BASE COUNT          491 a         1023 c         1039 g          463 t
ORIGIN      
        1 cccaagcccc gtgctcccgc cgcgacgctt acgcactggc gcatgagcgg gcctgcgaaa
       61 gcgaactcgc cgacgcgctg gccgaggacc tcgcccagga caggctgccg gatctggacg
      121 cgctccgccg tatcccaccg ccgagaaccg cctgctgttg ggtgctgatc tgagcgagac
      181 tggccgtcct tttggtgacg ggatggtggg aagtgtccac gaaggcgata atgggcacaa
      241 gcgtccggtc tgagcgacgg cgccggtcct ggcctgaggc gatcaagcgg gagatcgtgg
      301 cggcgtcgtt ggtgccgggt gcgtcggttt cggtcgtggc gcggcaatac gacgtgaacg
      361 ccaatcaggt gtttgcctgg cgcaagctgt atggcggcac ggctggggtg ccgccgggac
      421 ttcgcatggt gccggtgacg gtgacgccgg acccgccggc cgcgccatcg ccgccatcgc
      481 cgtggccgtc ggccgggatg atcgagatcg cgcttccggg cggctattgc gtgcgggtgg
      541 gctgcggcgt gacggcttcg gcgctgcggc gggtgctgga cgcgttggag cggcgatgat
      601 cgcctttccg gcgagcgtgc gggtgtggct ggcggtgggg cggaccgaca tgcggcgcgg
      661 catgaacggg ctggcgttgc aggtgcagca ggcgctcggg cgggacccgc atgccggcga
      721 tctttacgtc ttcagaggcg ccagaggcga cctgatcaaa attttctggc acgatggcgt
      781 cggcatgtcg ctctacgcca agcggctgga gaaaggtcgc ttcgtgtggc cgtcgccggc
      841 tgacggcgtc gtgggcattt cggcggcgca gctatcctat atgctggatg ggatcgactg
      901 gcgaaatccg cgccacacct ggcggcccgc gaccgccgga taatcccaaa cgtctctggc
      961 ggtaccgcgc tcactgtgat tccatcgggc catggacacg gacgccgcca cgcccgggcc
     1021 ggaagagttc gcagcactcc aggcagcgct cgcggcggcg caagcgcggg ctgaccaggc
     1081 cgaggcccag ctcgccgccg ccaacgcgcg gagctcggac gatcaggccc ggatcgccga
     1141 cctgcggctg cagatcgcca agctcaagcg ccagctgttc ggcacgcgat cggaacgcac
     1201 gcagcggctg ctcgatcagc tcgagctgca gctggaagag ttggagctcg acgccggcga
     1261 ggacgagatt gccgccgaac aggccgcggc caaaaccacg acggtggcag gcttcgtgcg
     1321 caagcggccg tcacgccagc ccttgcccgc gcatctgccg cgcgagcgcg tggtcgagcc
     1381 cgccccctgc gcctgcccgg cctgtggcgg cacccgcctg tcgaagctgg gcgaggatat
     1441 taccgagacg ctggaagtga tcccgcgcca gtggaaggtg atccagcgcg tgcgcgagaa
     1501 attctcctgc cgggattgcg aggtgatcac ccaggcgccg gcaccgttcc acgtcacccc
     1561 gcgcggctgg gctggcccca acttcctcgc catgctgttg ttcgagaaat tcggccagca
     1621 tcagccgctg caccgccagg cggagcgcta cgcccgcgaa ggcgtgacgt tgagcctgtc
     1681 gacgctggtc gaccagatgg gtgcgggtgc cgccgcgctg ctgccgatct accggcgcat
     1741 cgaggcgcat gtgctggcgg ccgagcgcct gcatggcgac gatacgaccg tgccggtgct
     1801 ggccaagggc aaaacggaca ccggccggct gtgggtttat gtgcgcgacg acatgccttt
     1861 cggggggcgc gcgccgccgg cggcgatttt ccattattcg cgtgaccggc ggggcgaaca
     1921 tcccagggcg catctcgcca gctggagcgg tgttctgcag gccgacgcct atggcggctt
     1981 cagcgacctc tatgcggccg gccgccagcc cgggccggtc gtggaagcaa gctgctgggc
     2041 gcacggacgg cgcaaggtgt tcgaacttgc cgatattgaa acagccgcca tcaggcaggc
     2101 ccgcggcgag acgcccaaac cggtctatcc gctggcactg gaagcggtgc gccgcatcga
     2161 cacgctgttc gacatcgagc gcgagatcaa tggcctgcca ccggagcagc gccgggccat
     2221 tcgccaagcc aggagcgcgc cggtcgtcgc cgagctcgaa acctggatga gcgaaacacg
     2281 cgacaaactc tcgcgcgggc atgatctggc caaggcgctg aactacatgc tgcgtcgctg
     2341 gccctccttc acgcgcttcc tcgaggacgg gcggatctgc ctcaccaaca acgccgccga
     2401 gcgcgcgttg cgcggcgtgg ccctcgggcg caaggcgtgg ctgttctgcg gttccgatcg
     2461 cggcggccag cgcgccgcca tcctctacag cctgatcgtc accgccaaaa tgaacggcgt
     2521 ggacccgcaa gcctggctcg ccgacgttct ggcccgcatc gccgggcatc ccatcaatcg
     2581 gctggacgag ttgctgccct ggaactggaa gcccgccaca tccgcacccg aagcggcggc
     2641 ggcatgagca cccagcgcaa caaggttcac cacgtccaca ccgtcgcacg cgtcgcccgg
     2701 cagctcggcg tccatgaaga tctcatccac accctcactc tcggcctgga acccgaggat
     2761 ggcgtcatct gggtttacgg cctcgacgat gatgacggcg tcctggcctt caccgatgac
     2821 ggcatcgagg aagtacggct catcctggca gaacacaccg aatatatccg ccgcaacccg
     2881 tcaaaatact gacctgcccg cggtcctgac cgactggata ccccgctcag cgcctacgac
     2941 gcgctgctga ccggagacgc ggcatgaccg gcgccgcccc gatccacgcg atcgacgcgc
     3001 agaggctcag cctcat
//
LOCUS       DASSZH010000533         5499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1171949, whole genome shotgun sequence.
ACCESSION   DASSZH010000533 DASSZH010000000
VERSION     DASSZH010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171949"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1497
                     /locus_tag="VFN42_11650"
     CDS             <1..1497
                     /locus_tag="VFN42_11650"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET8997312.1"
                     /translation="IQRIEVISGPGATLWGANAMNVVINIITRNSSDTQGGLITVGGG
                     NYELYDGIEYGGRLSPELTYRVHGEFTHFNAFPAAGGGNAGDAWYKPQGGFRLDWNPP
                     GQALSLQGDFFDAAEQPGGTVRGGDAMVSYNRDLSGGSHLQLLAYYDNAQRWSNNGLG
                     FAVNTYDLEIQHSFKFAGWNSVVWGAGERVVDYQIHFASTSSLRYEPARATLNLANIF
                     AQDTIGLAPSLKLTLGLKLEDEPYAGVQPMPSIRLGWTPANHVFFWAAVSRAVRSPTP
                     VDTTIQEFVGPRDILNGTTNFMPEKVTAYEIGTRVQVSRRASLSVSGFYNVYNDIRSL
                     DLGGPGSSSEFLFGNSVAATTVGVEAWGNYQVTDWWRISAGITAQHESFRFLPATLTA
                     HGYLPEQVGLSFVANDPGHLASLRSTMNFGHGVTLEAFLREVGELPHPVVPGYTELNA
                     RIGWQVNHRLNLSLSGFNLLHRYHLEFINPGQYTEVPRMIFAQAQVRF"
     gene            1561..2490
                     /locus_tag="VFN42_11655"
     CDS             1561..2490
                     /locus_tag="VFN42_11655"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HET8997313.1"
                     /translation="MSKIAVIGAGLIGRAWSIVFARAGFDVALWDKFPQAVEGARTFI
                     GERLPELHEAGLLAEQPGTVLARIRPAATLAEAVRDAAHVQENGPERVAEKQTLFAEL
                     DLAAPPDAVLASSTSGIPASAFTEGLAGRARCLVAHPVNPPYLIPLVELCPAPWTEPE
                     VVVRTRELMTRAGQVPATVKKEMDGFALNRLQGALLAEAFRLLADDVISPADLDALVK
                     HGLGLRWSFMGPLETIDLNAPGGLADYCKRYGPLYYAMQQQMQSLLWDPMLVGRLHDA
                     RRADLPANMMPVRQEWRDRRLMALLAHKAKQPE"
     gene            complement(2670..5156)
                     /locus_tag="VFN42_11660"
     CDS             complement(2670..5156)
                     /locus_tag="VFN42_11660"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HET8997314.1"
                     /translation="MDVPDTAASPARQSVVSRIALPAQDEDVASLRRLIVAKMTYAIG
                     RDPLVATDRDWFIATALAVRDRMVDRWLPATRTDYTRNRKRVYYLSLEFLLGRLLLDS
                     MNNLGLTEPMRAALGELGVDLDRLRAIEPDAALGNGGLGRLAACFMESMASLGIPAHG
                     FGIRYNHGLFHQVIRDGWQHEYPENWLSFGNPWEFERPDINYNIGFGGGVDQVPTTDG
                     PPRCVWHPQETVVAVAYDTPVVGWRGNHVNTLRLWSARAPDPLVLETFNRGDHVGALA
                     ARSQANAISQILYPSDETPAGQELRLRQEYFFTAASLQNLLYRHLRQYHTLTNLAEKC
                     SIQLNDTHPSIAVPELMRLLVDVHGMDWDQAWQITVNTVSYTNHTLLPEALETWPVPL
                     MERLLPRHMQIIYEINKLHLDSLAGRPDAPQLSAVSLIDENAGRRVRMGMLAFLGSHK
                     INGVSALHTELMRQTVFKPMHALFPNRIVNKTNGITFRRWLFTANPLLTGILRDVIGP
                     EMMDDAMALERLAPLADDTGLHDRLIAARHANKQALAGLIAERVDVVVSPDALFDVQI
                     KRIHEYKRQLLNILQTVALYNAMRARPTQDWVPRVKIFAGKAAASYHRAKLIIKLIHD
                     VAKVVNDDPALHGLLKVVFLPDYNVSLAESIIPAADLSEQISTAGMEASGTGNMKLAL
                     NGALTIGTLDGANVEIRDRVGAENMFIFGLTTEEVEQQRHRPRGAATPSPALQQVLDA
                     VGSGVFSPDEPLRYRELVADLMNNDWFMVTADFEAYAAVQDDVAARWRDRAAWWRSSV
                     LNTAHVGWFSSDRSIREYAEGIWNVPVLAE"
BASE COUNT          914 a         1790 c         1881 g          914 t
ORIGIN      
        1 atccagcgta tcgaggtcat cagcgggcct ggcgccacgt tgtggggcgc caacgcgatg
       61 aacgtcgtca tcaacatcat cacccggaat tccagcgata cccagggtgg tttgatcacg
      121 gtcggcggcg gcaattacga gctgtatgat gggattgagt acggcggccg gctcagcccg
      181 gaactgacct atcgcgtgca tggcgaattc acccatttca atgcatttcc cgctgccggc
      241 ggcggcaacg cgggcgacgc gtggtacaag ccgcagggcg gcttccgcct cgactggaac
      301 ccgccggggc aagcgctgtc gctgcagggg gatttcttcg acgcggcgga acagccgggc
      361 ggcaccgtcc gcggcggcga cgcgatggtg tcctacaacc gcgacctgag tggcggttcg
      421 catctgcaac tgctggccta ttatgacaac gcgcaacgct ggtcgaacaa tgggctgggc
      481 ttcgcggtca acacctacga cctggaaatc cagcacagct tcaagttcgc cggatggaac
      541 agcgtcgtct ggggcgccgg cgagcgcgtg gtcgattatc agatccactt cgccagcacg
      601 agcagcctgc gctacgaacc ggcgcgggcg acgctcaacc tggcaaatat cttcgcccag
      661 gacacgatag gcctggcacc gagcctgaag ctgacgctgg ggctgaaact ggaagacgag
      721 ccctatgccg gcgtgcaacc gatgcccagt atccgcctgg gctggactcc ggcgaatcac
      781 gtgtttttct gggcggcggt gtcacgcgcg gtccgttcgc cgactccggt ggataccacc
      841 atccaggaat tcgtcggacc acgcgacatc ctgaacggca cgaccaattt catgccggag
      901 aaagtgaccg cctacgagat tggcacgcgg gtgcaggtgt cgcgccgcgc gtcgttgtcg
      961 gtctctgggt tctacaacgt ctacaacgac attcgcagct tggacctggg cgggcccggg
     1021 tcgagctccg agttcttgtt cggcaacagc gtcgcggcga cgacggtcgg cgtggaggcc
     1081 tggggtaatt atcaggtgac cgactggtgg cgcatcagcg ccgggatcac cgcgcagcac
     1141 gagagcttcc gcttcctgcc ggccaccctg acggcgcacg ggtatcttcc tgagcaggtt
     1201 ggcctgagct ttgtcgccaa cgacccgggc cacctggcct cgctgcgttc cacgatgaat
     1261 ttcggccatg gggtgacgct ggaggcattc ctgcgcgagg ttggcgaact gccgcatccg
     1321 gtcgtgccgg gctataccga actcaacgcg cgcatcggct ggcaggtgaa ccaccggctg
     1381 aacctttcgc tgtccggctt caacctgctg caccgctacc atctggaatt catcaacccc
     1441 gggcaataca ccgaggttcc gcgcatgatc ttcgcgcagg cgcaggtgcg tttctgaaca
     1501 cggcggcggt tgcggcggcg cggcccgggt ggtgtcatcc ggcccgaagg agactcgcgc
     1561 atgagcaaga ttgccgtcat cggcgccggc ctgatcggcc gcgcctggtc cattgttttc
     1621 gcccgcgccg gattcgacgt ggcgctgtgg gacaagttcc cccaggcggt ggagggtgcg
     1681 cggaccttca tcggcgagcg cctgccggaa ctgcatgaag ccgggctgct ggccgaacag
     1741 cccggcaccg tactggcgcg gatccgcccc gccgccacgc tggccgaggc ggtgcgcgat
     1801 gcggcgcacg tgcaggagaa cgggccggag cgggtggcgg agaagcagac gctgttcgcc
     1861 gagctggact tggccgcgcc accggatgcg gtgctggcca gctcgaccag cggcattccg
     1921 gccagcgcct tcaccgaagg gcttgccggc cgcgcccgct gcctggtggc gcatccggtg
     1981 aacccgccct acctgatccc gctggtcgaa ctctgcccgg caccatggac cgagccggag
     2041 gtggtggtgc gcacccgcga gctgatgacg cgggcgggcc aggtgccggc gacggtgaag
     2101 aaggagatgg acggcttcgc gctgaaccgg ctgcaaggcg cgctgctggc ggaggcattc
     2161 cggctgctgg cagacgacgt gatctcgccc gccgatctcg atgcgctggt caagcacggg
     2221 ctgggcttgc gctggagctt catggggccg ctggagacga tcgacctgaa tgcgccgggc
     2281 gggctggcgg attactgcaa gcgctacggg ccgctgtact acgcgatgca gcagcagatg
     2341 cagtctttgc tgtgggaccc gatgctggtg ggccggctgc atgacgcgcg ccgcgcggat
     2401 ctgccggcga acatgatgcc ggtgcggcag gaatggcgcg accggcggct gatggcgctg
     2461 ctggcgcaca aggcaaagca gccggagtag gaaggcaggg aagcgcagat gaacgcggat
     2521 gaacgcggat attcccggcg gaagggggca gagtcgcgcg tattccggcg ctactgattc
     2581 cccaatcttt gtcttcggtg gggaataccc cgggaccggt caatccccac cgcagcatcc
     2641 gtgttcatcc gcggttccgt gatcgccccc tactccgcca gcaccggcac gttccagatg
     2701 ccctcggcat attcccggat gctgcggtcg gaggagaacc agccgacatg cgcggtgttc
     2761 agcacgctgg agcgccacca ggccgcccgg tcgcgccagc gcgcggcgac atcgtcctgc
     2821 accgcggcat aggcctcgaa atccgccgtt accatgaacc agtcgttgtt catcaggtcg
     2881 gccaccagtt cgcggtagcg cagcggctca tccggggaga acacgccgga acccaccgcg
     2941 tccagcacct gctgcagggc cggtgacggg gttgccgctc cgcgcggacg atggcgctgc
     3001 tgctccacct cctcggtggt caggccgaag atgaacatgt tctccgcccc gacacggtcg
     3061 cggatctcca cgttcgcgcc gtccagcgtg ccgatggtca gcgcgccgtt caacgccagc
     3121 ttcatgttgc cggtgccgga tgcttccatg cccgcggtgg agatctgctc ggacaggtcg
     3181 gcggccggga tgatggattc ggccaggctg acattgtagt ccggcaggaa caccaccttc
     3241 agcaagccgt gcagcgccgg gtcgtcgttc accaccttcg ccacgtcgtg gatcagcttg
     3301 atgatcagct tggcgcggtg atagctggcc gccgccttgc cggcgaagat cttcacccgc
     3361 ggcacccagt cctgcgtcgg ccgcgcccgc atcgcgttgt acaaagcgac ggtctgcagg
     3421 atgttcagca actggcgctt gtactcgtgg atgcgcttga tctgcacgtc gaacagcgcg
     3481 tccgggctca ccaccacatc gacgcgttcg gcgatcagcc cggccagcgc ctgcttgttg
     3541 gcatgccggg cggcgatcag ccggtcgtgc aggccggtgt cgtcggcgag cggcgccagc
     3601 cgctccagcg ccatcgcgtc atccatcatc tccggcccga tcacgtcccg cagaatgccg
     3661 gtcagcagcg ggttggcggt gaacagccag cgccggaacg tgatgccgtt ggtcttgttg
     3721 acgatgcggt tggggaacag cgcgtgcatc ggcttgaaca cggtctgccg catcagctcg
     3781 gtatgcagcg cggacacgcc gttgatcttg tgcgagccaa ggaaggccag catgcccatg
     3841 cggacccgcc gcccggcgtt ctcgtcgatc agcgacaccg cggacagctg cggcgcatcc
     3901 ggacgtccgg ccagcgaatc caggtgcagc ttgttgatct cgtagatgat ctgcatgtgg
     3961 cgcggcagca gccgctccat cagcggcacc ggccaggtct ccagcgcctc cggcagcaag
     4021 gtgtggttgg tgtaggagac ggtgttgacg gtgatctgcc aggcctgatc ccagtccatc
     4081 ccgtgcacgt ccaccagcag ccgcatcagc tccggcaccg cgatcgacgg atgggtatca
     4141 ttgagctgga tcgagcactt ctcggccagg ttggtcagcg tgtggtactg gcgcaggtgg
     4201 cggtagagca ggttctgcag cgaggccgcg gtgaagaaat actcctgccg cagccgcagc
     4261 tcctgtccgg cgggggtctc gtcgctgggg tacaggatct gcgaaatggc gttggcctgc
     4321 gagcgggcgg ccagcgcgcc cacgtggtcg ccacggttga aggtctccag caccagcggg
     4381 tccggcgccc gggccgacca caggcgcagc gtgttcacgt ggttgccgcg ccagcccacc
     4441 accggcgtgt cataggcgac cgcgaccacc gtctcctgcg gatgccacac gcagcgcggc
     4501 ggcccgtcgg tggtggggac ctgatccaca ccgccgccga agccgatgtt gtagttgatg
     4561 tcggggcgct cgaactccca cgggttgccg aaggagagcc agttctccgg gtattcgtgc
     4621 tgccagccgt cgcggatcac ctggtggaac aggccatggt tgtagcggat gccgaagcca
     4681 tgggcgggaa tgcccaggct ggccatgctc tccatgaagc aggcggccag ccggcccaga
     4741 ccgccattgc ccagcgcggc atccggttcg atggcacgca gccggtcgag atcaacgccc
     4801 aactcgccca gcgccgcccg catcggttcg gtaaggccga gattgttcat ggaatccagc
     4861 agcaggcggc cgagcaggaa ttccagcgac aggtagtaca cccgcttgcg gttgcgggtg
     4921 taatcggtgc gggtggcggg cagccagcgg tccaccatgc ggtcgcgcac cgccagggcg
     4981 gtggcgatga accagtcgcg gtcggtggcg accagcgggt cgcggccgat cgcataggtc
     5041 atcttggcga cgatcagccg gcggagcgag gcgacgtcct catcctgcgc aggaagcgcg
     5101 atgcgggaca caacgctttg ccgcgccgga gaagcagcgg tgtcaggcac gtccatgatg
     5161 ttacttggcc ccctgggtaa cgggcggacc gccgggtggg gcggtcgtgg ggaagcgaca
     5221 ggctccggaa cgcgcgggtc tagcaaacgc gtcccggtgc cgcacgggtt ttgttggacg
     5281 ccccggacgc cggatacgca tcccgccgcc aggatcgccg cttcggctta acaaagcgat
     5341 aatcgccggg atgaacttcc ggcgagccat ccgggaatac agcgggcgca ccgccctccg
     5401 gttccccctg ccagccgaac ggcgccgtga tgcaggcggg gctgcggccc agccgccaca
     5461 ccactccagt caagagcggc aagcacctga tcctgacgc
//
LOCUS       DASSZH010000534         3760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1410110, whole genome shotgun sequence.
ACCESSION   DASSZH010000534 DASSZH010000000
VERSION     DASSZH010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410110"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..3408
                     /locus_tag="VFN42_11665"
     CDS             337..3408
                     /locus_tag="VFN42_11665"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET8997315.1"
                     /translation="MLSVMFCDLVGSTELSSRMDPEDLHDLIRVYQACVTETIGRFGG
                     FIARYVGDGVLVYFGWPQASETDAERAVGAALETASAVAAQPVGGEPLQVRIGIASGL
                     VVVGHEVGEGQSREQTAIGETPNRAARLQALAGPGQIVIDAATRRLLGSLFDCRTLGD
                     VQLKGLPEPIEVFEAVAATPVQNRFKALRQTARSSMVDRVDEFALLLRHWERARAGRG
                     STILMSGEPGIGKSRLLAALDDRLRSEGELIRLRCFCSPYQQATPLHPIIGQLEFSAG
                     FDRDDTPEEKLRKLQVLLAEPPTQADDLALIAELLGLPVADSPHHNLSPLRRRERTYA
                     ALLRRVEMLADKQPLLLMLEDAHWADPSTIELLTQALPRLHARRALIVVTFRPEFQPP
                     WTEQQGATLVPLSRLDPADARQLAMQVIAGPALVSALLSQIVAHADGVPLFIEELTKG
                     MLETRRGDLSGPTAFAVPETLQASLMARLDRNPVARQVAQIGAVLGREFPHTLLAAVA
                     DMAEAQLLNGLAQLVTAGLLFCYGTAPDARYVFKHALIQEVAYESLLRRRRSVIHART
                     VEALERLSPETSAAQPELLAYHCSEAGLLRQAADYYRRAGEQALRRSAIPEARSHLER
                     GLQVIAALPDTVERQTVEAGLLLALGSVGIIQRGFGNAETAQAISQAVTQARRSGESR
                     LLVRALFGEWNYRSHIGDLAGSQTIAQEMLQLGRQQDEPLLRLVAGMTLGMNYTWAGR
                     FLQARTLFEECLQELDRRPVLGAGSPYPQDQQVLTRACLSLQLACLGYCDQAAAESRA
                     AIERARQLQHQPSLAVALTVGCRLAWLLRDTVLMRERATELVALCEEYGFPYWLARGR
                     AFTGLIMVTEGKPAPGLALMRDASATLEQSGILLWNMDGLLGEAYACAGDPDSAQRHL
                     QMALDVSARTGEAWLDAELLRQQGEIVANLPGTTPDAAETLLRRALVQAREQSARLWE
                     LRAAMSLARLLRRHGRTAEVRAVLEPVCDWFSAHGAAPGLGEADLLLTEVA"
BASE COUNT          606 a         1289 c         1285 g          580 t
ORIGIN      
        1 catgcttccg atagcgcgaa ggcacgggga ttacgccggc attggcgcga cgcaggcgcg
       61 gggacaagtg catgaaacgg ccagagacgc gggcaaacag tcggactgct tcgcgcgcga
      121 ctcaggactg acgacatgga atgtccgcgc tgcaagacca acgttctccc gggcagccgg
      181 ttttgtaacg aatgcggctc cgccatgccg caactgtgcg gagcgtgcgg cggcgaaaac
      241 cccgctgccg ccaaattctg ctctgcctgc ggcaccccgc tggccgctgc ggccgcccgg
      301 cgcgaagtgc ggaccgaacc ggcgcccgag cgccgcatgc ttagcgtcat gttctgcgac
      361 ctggtcggat ccaccgaatt gtcctcccgc atggacccgg aggatctgca cgacctgatt
      421 cgcgtctacc aggcctgcgt cacggagacg atcggccgct tcggcggctt catcgcgcgc
      481 tatgtcggcg atggcgtgct ggtctatttc ggctggccgc aagcatcgga gaccgacgcg
      541 gaacgtgccg tcggcgcggc gctggagact gcttcggcgg tggccgcaca accggtcggt
      601 ggcgagccac tgcaggtccg catcggcatt gccagcggcc ttgtcgtggt gggacacgaa
      661 gtcggcgagg gtcagtcgcg cgagcagacc gcgatcggcg aaacgccgaa tcgtgccgcg
      721 cgtctgcagg ccctggccgg ccccgggcag atcgtcatcg acgccgccac ccgccgcctg
      781 cttggcagcc tgttcgattg ccgcacgctg ggcgacgtgc agctcaaagg cctgcccgaa
      841 ccaattgaag tattcgaggc ggtcgccgcc accccggtgc agaaccggtt caaggcgttg
      901 cgacagaccg cccgcagttc aatggtggac cgggtggacg aattcgccct gctgctgcgg
      961 cactgggagc gcgcacgagc gggccgcggc agcaccatcc tgatgtcggg cgagcccggg
     1021 atcggcaagt cgcgcctgct ggctgcgctg gacgaccgct tgcgttcgga aggagagctg
     1081 atccggctgc gctgcttctg ctcgccgtac cagcaggcga cgccgctgca cccgatcatc
     1141 ggccagttgg aattctccgc cggtttcgat cgcgacgaca cgccagagga aaagctgcgc
     1201 aagctgcagg tgctgctggc cgagccgccg acgcaagcgg acgatctggc gctgatcgcc
     1261 gaactgctcg gcctgccggt cgccgattcg ccgcaccaca acctcagtcc gctgcgccgc
     1321 cgcgagcgca cctacgcggc gctgctccgc cgggtggaaa tgctggcgga caagcaaccg
     1381 ctgttgctga tgctggaaga tgcgcactgg gcggatccgt cgacgatcga actgctgacc
     1441 caggccctgc cgcgtctgca tgcgcgacgg gcgctgatcg tggtgacctt ccgtcccgaa
     1501 tttcagccac cctggacgga gcagcagggc gccacgctgg tgccgctcag ccgcttggat
     1561 ccggcagatg cccgccaact ggccatgcag gtgatcgccg gccccgcgct cgtttccgcg
     1621 ctgctgtcac agatcgtcgc ccacgccgac ggcgtgccgc tgttcatcga agagctgacc
     1681 aagggcatgc tggaaacgcg gcggggcgat ctgagcgggc caaccgcctt tgccgtgccg
     1741 gagacgttgc aagcctcctt gatggcgcgg ctggaccgca atccggtggc gcggcaggtc
     1801 gcgcagatcg gcgccgtgct ggggcgggaa ttcccccaca cgctgctcgc cgccgtggcc
     1861 gatatggcgg aagcgcaact gctgaacggg ctggcgcaac tcgtcaccgc cgggctgctg
     1921 ttctgctacg gcaccgcgcc ggacgcccgc tatgttttca aacacgcgct gatccaggaa
     1981 gtcgcctacg aaagcctgct gcgccgccgc cgcagcgtga tccacgcgcg tacggtggag
     2041 gcgctggagc ggctgtcgcc ggaaaccagc gcggcccagc cggaactgct cgcgtaccac
     2101 tgctcggagg cggggctgct ccgtcaggcc gcggattatt accgccgcgc cggcgaacag
     2161 gccttgcgcc gttcggcgat tcccgaagca cgctcgcatc tggaacgcgg gctgcaggtc
     2221 atcgccgcct tgcccgacac cgtcgaacgc cagaccgtgg aagcgggact gctgctggcg
     2281 ctgggcagcg tcggcatcat ccagcgaggc ttcggcaatg cggagaccgc gcaggccatc
     2341 agccaggcgg ttacgcaggc gcggcgcagc ggcgagagcc ggctgctggt gcgggcactg
     2401 ttcggcgaat ggaactaccg ctcccatatc ggcgacctgg cgggatcgca gaccattgcc
     2461 caggagatgc tgcagctcgg ccgccagcag gacgaaccgc tgctgcgcct ggtggccggc
     2521 atgacgctgg gcatgaacta cacctgggcc ggccgcttcc tgcaggcccg cacgctgttc
     2581 gaggaatgcc tccaggaact ggaccgccgt ccggtgctgg gcgccggcag cccctatccg
     2641 caggaccagc aggtattgac ccgcgcgtgt ctctcgctgc agctcgcctg cctcggttac
     2701 tgcgaccagg cggccgccga gagccgggcg gcaatcgagc gcgcacgcca gttgcaacat
     2761 cagccgtcgc tggcagtggc cctgaccgtc ggctgccggc tggcgtggct gctgcgcgac
     2821 accgtgctga tgcgcgaacg cgccaccgag ttggtggcgc tgtgtgagga atacggcttc
     2881 ccctattggc tggcgcgcgg acgcgccttc accggcttga tcatggtcac cgagggcaag
     2941 cccgcacccg gcctggcact gatgcgggat gcctcggcga cgctggaaca atccggcatc
     3001 ctgctgtgga acatggatgg actgctgggc gaggcttacg cctgcgcggg cgatcccgac
     3061 tcggcccagc gccatctgca gatggcgctg gacgtttccg cacgcaccgg cgaggcatgg
     3121 ctcgatgcgg aactgctgcg ccagcagggg gaaatcgtcg ccaacctgcc cggcacgacc
     3181 ccggatgcag ccgaaaccct gctgcgccgc gccctggtgc aggcgcggga acaatcggcg
     3241 cggctatggg aattgcgcgc cgcgatgagc cttgcccggc ttctgcggcg gcacggccgg
     3301 acggcggaag tgcgcgccgt gctggagccg gtgtgcgact ggttcagcgc ccacggcgcc
     3361 gctcctggcc tgggcgaggc agacctgctg ctgaccgagg tagcctgagc gcccatccgc
     3421 gctagccgca ggcaccggtg cgccgttcgg tggtggtggc aatccgccag agcgcccgga
     3481 cgggcggcaa tcccgctcgc atgaatgggc gcgacccggg cgctatttct gaactggaac
     3541 cttgcacttc gcgcatggct tgtgcaacat caaacctgaa ataaccgact cgcgccaaca
     3601 cgcgggcgga cgatgtgtga ttgttcgccg atcgtcactc tgcacttgca agccggagga
     3661 aacccgtcat gggagcggcc gcagcccgta ccaaactcgc cgccgacatc aaccgcgcct
     3721 tcaatgcccg gaacatcgcc aattcggccc agcaggtcaa
//
LOCUS       DASSZH010000535         2436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_915735, whole genome shotgun sequence.
ACCESSION   DASSZH010000535 DASSZH010000000
VERSION     DASSZH010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915735"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..1775)
                     /locus_tag="VFN42_11670"
     CDS             complement(168..1775)
                     /locus_tag="VFN42_11670"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HET8997316.1"
                     /translation="MTAGNAIVDVLIIGSGASGAAVAWSLAETRMKILCLEQGDWMKS
                     TDFPSNGRDWEARRYGDFDISPNRRGRDTDYPVNDDNSVMKVANFNGVGGGTLLYTAH
                     YPRMHPSDFRVRSLDGVADDWPVDYWTLEPYFAENDRMMGVSGLAGDPAYPAHQPLMP
                     PLPLGRNGMRYARAMNQLGWHWWPSDTTVATTEYDGRAPCINLGHCTPGCAQGAKASV
                     DITYWPKALRAGVELRTRCRVREITTNEHGMASGAIYYDPDGVERFQPAEVVILACNG
                     IGTPRLLLNSASARWPNGLANSSGLVGKNLMLHPWPQVRGYVDEEMDGDRAPMTVLWS
                     KQFYETDPARGFVRGYTLQFARGTGVVNEAITSTAAGLLPWGKEHHSVYRRLLYRRLL
                     IGVACEDLPEEHNQVTLDPVLKDSHGIPAARIDYTISENTRRMMEHGIARATDILTAA
                     GATDLYTSRTILNSPGHLLGTARMGNDPDRSVVNAWGRSHDVKNLFIVDGSIWVTSGG
                     VNPTSTIQALALYIADSMKQRLATLFD"
     gene            complement(1772..2227)
                     /locus_tag="VFN42_11675"
     CDS             complement(1772..2227)
                     /locus_tag="VFN42_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997317.1"
                     /translation="MHSDADAALTPEQMLDFRRLAGLMIPPDQGLGLPGADDAAIFAD
                     IVRSLGRDADHVRTALTHLAALAGGSFAECDAATRDSIAATLLAGDGPEIAALGRAVL
                     QCYYRDDRVMQSLGLEPRPPFPKGHTIEQGDWSLLDVVRARPKMWRDAP"
BASE COUNT          352 a          815 c          852 g          417 t
ORIGIN      
        1 gtgggcgctg ctgatgctgg aactctggtt ccgcatgtgg gtggaccagc cggccgagag
       61 cgcgctgctg ccgccggcgc tggccgcgta acgggcggcg cgcgggcggg tcgggcggtg
      121 atggggcgcg gggatgctgt cgcgcgccac tggcaaggcc gccggcctca atcgaacagc
      181 gttgccagcc gttgcttcat gctgtcggcg atgtagagcg ccagcgcctg gatcgtcgag
      241 gtcgggttca ccccgcccga ggtcacccag atgctgccgt cgacgatgaa caggttcttc
      301 acgtcgtggc tgcggcccca cgcattgacc accgagcggt cgggatcgtt gcccatccgc
      361 gcggtgccga gaaggtgccc cggactgttg aggatcgtgc gcgacgtgta gaggtcggtg
      421 gcgcctgccg cggtcaagat gtcggtggcc cgcgcgatgc cgtgctccat catgcgccgc
      481 gtgttctcgc tgatcgtgta atcgatgcgg gctgccggga tgccgtggct gtccttcagc
      541 accgggtcga gcgtcacctg gttgtgctcc tccggcaggt cctcgcaggc gacgccgatc
      601 agcagccggc gatacagcag gcggcggtaa acgctgtgat gctccttgcc ccagggcagc
      661 aggccggcgg cggtgctggt gattgcctcg ttcaccacgc cggtgccgcg ggcgaattgc
      721 agcgtgtagc cacgcacgaa gccgcgggcc ggatcggttt cgtagaactg cttgctccac
      781 agcaccgtca tcggcgcgcg atcgccgtcc atctcctcgt ccacatagcc gcgcacctgc
      841 ggccagggat gcagcatcag gttcttgccg accaggccgc tggaattggc cagcccgttc
      901 ggccagcgcg ccgaggcgga attcagcagc agccgtggcg tgccgatgcc gttgcaggcc
      961 aggatcacca cctccgccgg ctggaagcgt tcgaccccgt ccggatcgta gtagatcgcg
     1021 ccggacgcca tgccgtgttc gttggtggtg atctcccgca cgcggcagcg cgtgcgcaat
     1081 tccacgccgg cgcgcagcgc cttcggccag taggtgatat cgacgctcgc cttggcgccc
     1141 tgcgcgcagc ccggcgtgca gtggccgaga ttgatgcacg gcgcgcgccc gtcatactcg
     1201 gtggtggcaa ccgtggtgtc ggatggccac caatgccagc ccagttggtt catcgcccgg
     1261 gcatagcgca tgccgttgcg gccgagcggc aggggcggca tcagcggttg gtgcgccgga
     1321 taggcgggat cgccggccag gccggacacg cccatcatgc ggtcgttctc ggcgaaatac
     1381 ggctccagcg tccagtaatc gaccggccag tcatcggcca cgccgtccag cgaacgcacg
     1441 cggaaatccg acggatgcat ccgcggataa tgcgcggtgt acagcagggt gccgccgccc
     1501 acgccgttga agttggccac cttcatcacg gagttgtcgt cgttcaccgg ataatcggtg
     1561 tcgcgtccgc gccggttggg gctgatgtcg aaatcgccgt agcgccgcgc ctcccagtcg
     1621 cggccattgc tggggaagtc ggtggacttc atccagtcgc cctgttccag gcacaggatc
     1681 ttcatgcgcg tctcggccag gctccacgcc accgcggcgc cggatgcgcc ggaaccgatg
     1741 atcagcacat cgacgatggc gttacccgcg gtcacggtgc gtcgcgccac atcttcggcc
     1801 gcgcgcgcac cacatcgagc agcgaccagt cgccctgctc gatcgtgtgt cccttcggga
     1861 agggcggccg cggttccagt cccagcgact gcatcacgcg gtcgtcgcgg tagtagcact
     1921 gcagcaccgc gcgcccgagt gccgcgattt ccggcccatc gccggcaagc agcgtggctg
     1981 cgatcgagtc gcgggttgcg gcgtcgcatt cggcgaaact gccgccggcc aatgcggcca
     2041 ggtgggtgag ggccgtgcgc acgtggtccg cgtcccggcc aagcgagcgc acgatatcgg
     2101 cgaagatcgc ggcatcgtcg gcgccgggca ggcccagccc ctggtcgggt ggaatcatga
     2161 gcccggcgag ccggcggaaa tccagcatct gttccggcgt gagagccgca tccgcgtcgc
     2221 tgtgcatggc atggcactcg gttggggcct gcattcgatc tgcccagccc gggcggtgtc
     2281 aatccatgcg gcgggaatgc tgcccgtcat tcccctctcc gcccgcgcgg cggagagggg
     2341 aacagggtca gttggtgacc tgtttccagg cctgttccgc ggccggcggg agcttgtcca
     2401 tgtgcttcag gaacagcgcc agcgtccaca tctgct
//
LOCUS       DASSZH010000536         3280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_404798, whole genome shotgun sequence.
ACCESSION   DASSZH010000536 DASSZH010000000
VERSION     DASSZH010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404798"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..984
                     /gene="mreC"
                     /locus_tag="VFN42_11680"
     CDS             127..984
                     /gene="mreC"
                     /locus_tag="VFN42_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET8997318.1"
                     /translation="MLRLSIQTRQALAKLTLPVLVATSFGLMLLGKADAVLADRARMA
                     LADALVPIYAELAQPLASVRSAVSEVQDLWSLRAENARLRAENEQLRRWQSIALSLDA
                     QNRELKANLHWMPQPAPSFVTVPVVADAGGEYAKAVLLAVGPHHGVHPGEIALDATGL
                     VGRVTEVGKRTARVLLITDMNSRIPVMLENSHAHAILVGTNGPYPRLVFYPEGSPPQE
                     GERVVTTAEAKVFPANLPVGTVRHTASGTPEVVPDAHLNRLEMVRLFDYGLTGIVPPE
                     TARVAERQH"
     gene            984..1538
                     /locus_tag="VFN42_11685"
     CDS             984..1538
                     /locus_tag="VFN42_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HET8997319.1"
                     /translation="MAYSEDRPGIRPRQSWGRRLDLMARHSFPTNSTVLLMLICQAPF
                     GIVDQAELLPSVTLACVWFWSLFRPAAMPPPVVFLIGLLLDLLGYFPLGVGVLCTLLV
                     HGCALSWRRGLGRQGFAVIWLVFAVVAAIAAMLGWALTMLLTFRLLQPGPALFQAMLS
                     AALYPALAVPLARAHRTIADPGRA"
     gene            1554..>3280
                     /gene="mrdA"
                     /locus_tag="VFN42_11690"
     CDS             1554..>3280
                     /gene="mrdA"
                     /locus_tag="VFN42_11690"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET8997320.1"
                     /translation="MRREIRRTSVFTRRALLLMGGQVAVLGTLGARLYQLQVVDAARY
                     KTLANDNRISARLVAPPRGRILDRFGIPVADNKPNWRALLIAERTNDVRATLDRFSTL
                     VPLDDRERERIQRDLHRNRSFIPVLVRDYLTWDEMARIEVNAPELPGIVVDAGSTRNY
                     PFGPQLAHLIGYVGPPNERDLSNDPLLSLPGIRVGRTGVEKYHDLALRGRAGVVQLEV
                     NAVGRVVRELSRQDGVPGDTIGLTIDSALQQAALQHLGDQSGSAVVLDCTNGEILAMA
                     TNPSFDPGLFNSGVPQAQWNAWMQDPMHPLMNKATRGLYAPGSTFKMTVALAAQQTGA
                     VSPTQRFFCPGYFEVGDRRFWCWRRGGHGWLDMVGGLKNSCDVYFYQAALHTGIDPIA
                     GVAHRLGLGVDLPKLDLPEQRTGLVPTREWRKKQGHPWQLGDTVVAGIGQGYIEVTPV
                     QLATYVSRIATGRAVQPHVTRSLSGVEQPGMKPEDWPMLDIPDRQLQVARQGMWEVVN
                     DPRGTAPAAKLPDPRWQMAGKTGSAQVVAVPRALRLSGHFNSADLPWKYRPNALFVAF
                     APFDAPRYA"
BASE COUNT          473 a         1155 c         1136 g          516 t
ORIGIN      
        1 cgccactcct gacgccggcc ggcctgtgtc ccgcgcgacg cgggcgcatt ttctttccct
       61 gccatcccgc ggcaagcgcg aagaagcgta tgctcacggc accgaagttt gctacctggg
      121 cgcgcgatgc ttcgcctctc catccagacc cggcaggcac tggccaagct gacgctgccg
      181 gtgctggtgg ccacatcctt cggcctgatg ctcctgggca aggccgacgc cgtgctggcc
      241 gaccgcgccc gcatggccct ggccgatgcc ctggtgccga tctacgccga gctggcacag
      301 ccgctcgcca gcgtgcgctc cgcggtgtcg gaggtgcagg atttgtggtc gctgcgtgcc
      361 gagaacgcgc ggctgcgggc ggagaatgag cagttgcgcc gctggcagtc gattgccctg
      421 tcgctggacg cgcagaaccg ggagctgaag gccaatctgc actggatgcc acagccggcg
      481 ccgtccttcg tcaccgtccc ggtcgtggcc gatgccggcg gagagtatgc caaagccgtg
      541 ctgctcgcgg tcggtccgca tcatggcgtg catcccggag agatcgcgct ggacgccacc
      601 gggctggtcg gccgcgtcac cgaagtgggc aagcgcaccg cgcgcgtcct gctgatcacc
      661 gacatgaaca gccgcatccc ggtgatgctg gagaactcgc acgcccacgc catcctggtc
      721 ggcaccaacg gcccttatcc gcggctggtg ttctatccgg agggaagccc gccgcaggaa
      781 ggcgagcggg tggtcaccac tgccgaggcc aaggtgttcc cggccaacct gccggtggga
      841 acggtgcgcc ataccgcttc cggcacgccg gaggtggtgc ccgacgccca tctgaaccgg
      901 ctggagatgg tgcggctgtt cgactacggc ctgaccggca tcgttccgcc ggagaccgcc
      961 cgggtggcag aacggcagca ctgatggcct attcggaaga ccgccccggg atacggccgc
     1021 gccagagctg gggccggcgc ctcgacctga tggctcggca cagcttcccg accaacagca
     1081 ccgtcctgct gatgctgatc tgccaggcgc ccttcggcat cgtggaccag gcggaattgc
     1141 tgccgtcggt caccctggcc tgcgtctggt tctggtcgct gttccgtcca gccgccatgc
     1201 cgccgccggt ggtgttcctg atcggcctgc tgctcgacct gctcggctac ttcccattgg
     1261 gggtcggcgt gttgtgcacg ctgctggtgc acggctgcgc gctgagctgg cggcgcggcc
     1321 tgggacggca gggcttcgcg gtgatctggc tggtgttcgc cgtcgtcgcc gccatcgcgg
     1381 cgatgctcgg ctgggcactc accatgctgc tgaccttccg cctgctgcag cccgggccgg
     1441 cgctgttcca ggcgatgctg tccgccgcgc tttaccccgc cctggcggtg ccgctcgcgc
     1501 gcgcccaccg caccatcgcc gatccggggc gcgcatgaag gcgatggcct gacatgcgcc
     1561 gggaaatccg ccgtaccagc gtgttcaccc gccgagcgct gctgctgatg ggggggcagg
     1621 tggccgtgct ggggaccctg ggcgcacggc tgtaccagct ccaggtggtg gacgccgctc
     1681 gctacaagac gctggccaac gacaaccgga tcagcgcccg cctggtggcg ccgccgcgtg
     1741 ggcgcatcct ggaccggttc ggcattccgg tcgccgacaa caagcccaac tggcgcgcgc
     1801 tgctgatcgc cgagcggacc aacgacgtgc gtgccacact ggaccggttc tcgacgctgg
     1861 ttccgctcga tgaccgcgag cgggaacgca tccagcgcga cctccaccgc aaccgcagct
     1921 tcatccccgt gctggtgcgc gactacctca cctgggacga gatggcgcgc atcgaggtga
     1981 acgcgccgga actccctggg atcgtggtcg atgccggcag cacgcggaac tacccgttcg
     2041 gtccgcagct tgcccacctg atcggctatg tcgggccacc caacgaacgt gacctgtcca
     2101 acgacccgct gctgtcgctg cccggcatcc gcgtgggccg caccggcgtg gagaaatacc
     2161 atgatctcgc gctgcgcggc cgcgccggcg tggtgcaact ggaggtgaac gcggtcggcc
     2221 gcgtggtgcg cgaactgtcg cggcaggacg gcgtgccggg ggacacgatc ggcctgacca
     2281 tcgactccgc cctgcagcag gccgcgttgc agcacctggg cgaccagagc ggcagcgccg
     2341 tggtgctgga ctgcaccaac ggcgaaatcc tggcgatggc gaccaacccg tcctttgacc
     2401 cgggcctgtt caattccggc gtgccgcagg cgcagtggaa tgcctggatg caggacccca
     2461 tgcatccgct gatgaacaag gcgacccgcg ggctgtatgc gcccggctcc acattcaaga
     2521 tgaccgtcgc gctggcggcg cagcagaccg gcgccgtcag cccgactcag cggttcttct
     2581 gccccggcta tttcgaggtc ggcgaccgcc ggttctggtg ctggcggcgc ggcggccatg
     2641 gctggctgga catggtcggc ggcctgaaga acagctgcga cgtgtatttc taccaggctg
     2701 cgctgcacac cggcatcgac ccgatcgcgg gggtggcgca ccggctcggc ctgggggtgg
     2761 acctgccgaa gctggacctg cccgagcagc gcaccggcct ggtgcccact cgcgaatggc
     2821 gcaagaagca ggggcacccc tggcagttgg gcgacaccgt ggtggccggt atcggccagg
     2881 gctacatcga ggtgaccccg gtgcaactcg ccacctacgt gtcgcggatc gccaccggcc
     2941 gagcggtgca gccgcatgtc acccgcagcc tgtccggggt ggagcagccg ggcatgaagc
     3001 cggaggactg gccgatgctg gacatcccgg accggcagct tcaggtcgcg cgccagggca
     3061 tgtgggaggt ggtgaacgac ccgcgcggca cggcacccgc cgccaagctg ccagacccgc
     3121 gctggcagat ggccggcaag accggctccg cccaggtggt ggcggtgccg cgggccttgc
     3181 gcctcagcgg ccacttcaac agcgccgacc tgccgtggaa gtaccggccg aacgcgctgt
     3241 tcgtcgcctt cgcgccattc gacgcgccgc gctatgcgat
//
LOCUS       DASSZH010000537         4106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_166263, whole genome shotgun sequence.
ACCESSION   DASSZH010000537 DASSZH010000000
VERSION     DASSZH010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166263"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..1176)
                     /locus_tag="VFN42_11695"
     CDS             complement(196..1176)
                     /locus_tag="VFN42_11695"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET8997321.1"
                     /translation="MKTLIFDEPNKPVVTQVQMPGMTANDVLIRSRRVGICHSDYELL
                     AGRYIIPISYPVTPGHEWVGEVVEVGANVKGLAQGDRVVGECVIRTPERIHHFGFSMD
                     GADREFFTARPEWLHRLPDAVDDAKGALIEPFTCGYYAVMRHGGVNAADSVVVSGGGT
                     IGLVTAAAAIGMGARVIVVDPVPLRRDIARRLGAEAAVDPSAGDPVAAVQELTKGGAD
                     LVVECAGHAASLANVFDYARPEGNVSMVGINIGQKFPVELGKIQIKNLSVKGCIGSPG
                     VWPAAIRFLERTGIDLSPIQTHSFPIDQALAALELGKDPQQAVKVTLEIA"
     gene            complement(1205..2215)
                     /locus_tag="VFN42_11700"
     CDS             complement(1205..2215)
                     /locus_tag="VFN42_11700"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET8997322.1"
                     /translation="MTAEPVRIALIGMGWWGRKMLSVLAAAPADIRVVRAVEPNVETV
                     RELCAASGIALTAEYADALNDPAVEAVVLATPHALHEAQIAQAVAAGKHVFCEKPLAL
                     TRAGAERAVALCREAGLVLGMGHERRWEPPVADLLAKADAGVLGRIHQIEANFSHDKF
                     LSLDRDNWRLKADQAPAGGMTATGIHLLDLSVRLLGPAESVLCICERLSSDLPQGDTV
                     AAYAKFKGGGTSYVSASLANPFMSRFTVYGAKGWIDIRDKAHVEAPEGWVVTSAMAGG
                     PITTVEVPPAEPVKDNLVAFARAIRGAGSYPITAEHLVNTIALLEAVFASALSGRIER
                     VA"
     gene            complement(2212..2496)
                     /locus_tag="VFN42_11705"
     CDS             complement(2212..2496)
                     /locus_tag="VFN42_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET8997323.1"
                     /translation="MHYVIHCLDHPGAVEKRLAHYEAHKAYLAAAKIRTVISGPLLAD
                     DEQTMIGSCFVVEASSLAEVEAFNRNDPFHAIGLWQTISIRPFNKRVDNR"
     gene            complement(2518..3588)
                     /locus_tag="VFN42_11710"
     CDS             complement(2518..3588)
                     /locus_tag="VFN42_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8997324.1"
                     /translation="MNDGNIALIVGATGLSGSYAGRHLKGQGWTVVTVSRSAADLPWS
                     DRHIAVNLEDAAAAKAALAAASDVTNVFYCTWSRQANEEENVRVNAAMISNLFNGIEA
                     APLRHAALVTGLKHYLGSFDDYAKVKPYTPFLESSPRLPGPNFYYAQEDVLFAMAARR
                     GFAWSVHRPHTMIGFVIGNAMNMAVTLAVYAAICKHTGRPFVFPGSPEQYNAVTDVTD
                     ARLLAKQLHWAATTPEAANLPFNTVNGDLFRWTWLWRQIADYFGLPVGDYPGHPVPLE
                     TQMADAPAIWAEIVAKHGLQDIPVGRLASWWHSDADLGRTLECFTDMTNSRTRGFTAY
                     QPTPRSFFDVFDELRARRIIPA"
     gene            3906..>4106
                     /locus_tag="VFN42_11715"
     CDS             3906..>4106
                     /locus_tag="VFN42_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997325.1"
                     /translation="MQLDNHAAPQEAETVEAMPHKVDILPLHVRIDAVIADVTLNDAL
                     QYGTQVFFKPGDLNVSLSQHAAC"
BASE COUNT          665 a         1436 c         1327 g          678 t
ORIGIN      
        1 ccgtcgcgat ggcggagggc gcgaagaccg cgcacagcag cagcagcgcc agcgtcgaga
       61 ccaccaccgt caggaagccc ctggcgagcg gcacgccgag gatgccacgc ggccgggcca
      121 tgcggatcgg cttgtcagcg gtcaggttca tcgccctcgt ttcccccgat gtcgccgccg
      181 tgtcggcagg cacgctcagg cgatctccag cgtgactttc accgcctgct gcgggtcctt
      241 gccgagttcc agcgccgcca gcgcctgatc gatcgggaag ctgtgcgtct ggatcggcga
      301 gagatcgatg cccgtccgct ccaggaagcg gatcgcggcg ggccagacgc cgggcgagcc
      361 gatgcagccc ttcaccgaca ggttcttgat ctggatcttg cccagctcca ccgggaactt
      421 ctggccgata ttgatgccga ccatcgagac gttgccctcg ggccgcgcgt agtcgaagac
      481 attggccagc gaggcggcat ggcccgcgca ttccaccacc agatcggcgc cgcccttggt
      541 cagctcctgc accgccgcga cgggatcgcc ggccgagggg tccaccgccg cctcggcgcc
      601 gagcctgcgg gcgatgtcgc ggcgcagcgg caccgggtcc accacgatca cccgcgcgcc
      661 catcccgatc gccgccgccg ccgtcaccag cccgatggtg ccgccgcccg aaaccaccac
      721 gctatcggcc gcattcaccc cgccgtggcg catcaccgcg taatagccgc atgtgaaggg
      781 ttcgatcagc gcgcccttcg cgtcgtccac cgcatccggc agcctgtgca gccactccgg
      841 ccgggcggtg aagaactcgc ggtcggcgcc gtccatcgag aagccgaaat ggtggatgcg
      901 ctcgggcgtc ctgatcacgc attcgcccac cacgcggtcg ccttgggcga ggcccttcac
      961 gttggcgccg acctcgacca cctcgcccac ccattcgtgg cccggcgtca ccgggtagga
     1021 gatcgggatg atgtagcgcc cggcgaggag ttcgtaatcc gaatggcaga tgccgacgcg
     1081 gcgcgagcgg atcagcacgt cgttcgcggt catgccgggc atctgcacct gggtcacgac
     1141 cggcttgttc ggctcatcga agatcagggt tttcattggt tcctcccaaa ggtccggcgg
     1201 tcggtcaggc cacgcgttcg atcctgccgc tgagcgcgga ggcgaacacc gcctccagca
     1261 gggcgatggt gttcaccaga tgctcggcgg tgatcgggta gctcccggcg ccgcggatcg
     1321 cccgcgcgaa cgcgacgaga ttgtccttca ccggctcggc cgggggcacc tcgaccgtgg
     1381 tgatcggccc gcccgccatc gccgaggtga cgacccagcc ctccggcgcc tcgacatgcg
     1441 ccttgtcgcg gatgtcgatc cagccttttg cgccatagac ggtgaagcgc gacatgaacg
     1501 ggttggcgag cgaggccgag acgtagctgg tgccgccgcc cttgaacttc gcataggccg
     1561 ccaccgtgtc gccctgcggc aggtccgatg acaatcgctc gcagatgcag agcacgcttt
     1621 ccgctggccc cagcaggcga accgaaagat cgagcaggtg gatgccggtt gccgtcatcc
     1681 cgcccgccgg cgcctgatcg gccttcagcc gccagttgtc gcggtcgagc gacaggaact
     1741 tgtcgtggct gaaattggcc tcgatctggt ggatgcggcc cagcacgccg gcatcggcct
     1801 tggccagcag gtcggccacc ggcggctccc accgccgctc gtgccccatg ccgagcacaa
     1861 gccccgcctc gcggcacagc gcgaccgccc gctccgcccc cgccctggtc agcgccagcg
     1921 gcttttcgca gaacacatgc ttgcccgccg ccaccgcctg cgcgatctgc gcctcgtgca
     1981 gcgcatgcgg cgtggccagc accaccgcct ccaccgccgg atcgttcagc gcatcggcat
     2041 actccgccgt cagcgcgatg ccgctggccg cacacagttc gcgcactgtc tccacgttcg
     2101 gctcgaccgc gcgcaccacg cggatgtcgg ccggcgcggc tgccagcacg ctcagcatct
     2161 tcctgcccca ccagcccatg ccgatcaggg cgatccggac tggctccgcg gtcaccggtt
     2221 gtccacgcgc ttgttgaacg ggcggatcga gatggtctgc cacagcccga tggcgtggaa
     2281 cggatcgttg cggttgaacg cctccacctc ggcgaggctg ctggcttcca ccacgaagca
     2341 gctgccgatc atggtctgct cgtcatcggc cagcagcggg ccggagatga cggtcctgat
     2401 cttcgcggcg gccagatagg ccttgtgcgc ctcgtaatgc gccagccgtt tttccacggc
     2461 gccgggatgg tcgaggcaat ggatgacgta atgcatgggc gcggtccttc cgggtggtca
     2521 ggcggggatg atccgccgcg cgcgcagttc gtcgaacacg tcgaagaagc tgcgcggcgt
     2581 gggctggtag gcggtgaagc cgcgggtgcg gctgttggtc atgtcggtga agcattcgag
     2641 cgtgcggccg aggtcggcat cgctgtgcca ccacgaggcg aggcggccga cgggaatgtc
     2701 ctgcagcccg tgcttcgcca cgatctcggc ccagatcgcg ggcgcgtcgg ccatctgggt
     2761 ttcgagcggc accggatggc cgggatagtc gcccaccggc aggccgaaat aatccgcgat
     2821 ctgccgccac agccaggtcc agcggaagag gtcgccgttg acggtgttga acggcaggtt
     2881 tgccgcctcg ggcgtggtcg ccgcccagtg cagctgtttc gccagcagcc gcgcgtcggt
     2941 cacgtcggtg acggcgttgt actgctcggg cgagccggga aagacgaagg gccgccctgt
     3001 gtgcttgcag atcgcggcat agaccgccag cgtcaccgcc atgttcatcg cattgccgat
     3061 gacgaaaccg atcatcgtgt gcggccggtg aaccgaccag gcaaaaccgc gcctggccgc
     3121 catcgcgaac agcacgtctt cctgggcgta gtagaagttc ggccccggca ggcgcgggct
     3181 gctttcgaga aacggcgtgt agggcttcac cttggcgtaa tcgtcgaacg agccgaggta
     3241 atgcttcagc cccgtcacca gcgccgcatg gcgcagcggc gcggcctcga tgccattaaa
     3301 cagattgctg atcattgcgg cgttgacgcg gacattttcc tcctcgttcg cctgccgcga
     3361 ccaggtgcag tagaagacat tcgtcacatc cgacgcagcc gccagcgccg ccttcgccgc
     3421 cgccgcgtct tcaaggttca cggcgatgtg ccggtccgac cagggcagat ccgccgcgct
     3481 gcgcgatacc gtgacaacgg tccagccctg ccccttcagg tgacggcccg cgtagcttcc
     3541 ggaaagcccg gtcgcgccca cgatcagtgc aatattgccg tcgttcattt cttccgcctc
     3601 tcgtctgccg accgtgcgat gaggcgtcag gcccctggat ccgtgattgc gcagtcgccc
     3661 gagtcccagc acgaaccagc acccgagctt acggaaccca cctgaattcc aacccccgat
     3721 cgagcggcct ccccattgaa ccggccgcac gtcccattcg ggatcggtcc cgagccggtg
     3781 gcatcaagct aacaagaaat cgcccctggt cttcgattgt tttgtccaaa tcggataaat
     3841 caaatccgca tggagggcgg gctggccgaa ggacattcat ccgaaatgga cattttcggt
     3901 gtcggatgca gctcgacaat catgccgcgc cccaggaggc ggaaacggtg gaagcgatgc
     3961 cgcacaaggt ggacatcctg ccgctgcatg tgcgcatcga cgcggtgatc gccgacgtga
     4021 cgctgaacga cgcgctgcaa tacggcacgc aggtcttctt caagccgggc gacctgaacg
     4081 tctcgctgag ccagcacgcc gcatgt
//
LOCUS       DASSZH010000538         4591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_830754, whole genome shotgun sequence.
ACCESSION   DASSZH010000538 DASSZH010000000
VERSION     DASSZH010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830754"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VFN42_11720"
     CDS             <1..733
                     /locus_tag="VFN42_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003624393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HET8997326.1"
                     /translation="KLNRGHAVLIDILGADHGGYVKRMQAAVQALSGGEAKFEAVLCQ
                     IVHIIRAGQPVRMSKRAGSYVTLRDLLDEVGKDAVRFTMLTRKADAQMEFDLDQVVAQ
                     TRDNPVFYVQYAHARCRSVLRAAAEMFDAATISDAALAGLPLDSLAAEPELALIRRLA
                     AWPRTVEAAAQAREPHRIAFFLYDLAADFHMLWNRGKDDATLRFLQSDRPAETLARLA
                     LVAATAAVIRSGLAVMGVAPVEEMR"
     gene            814..1605
                     /locus_tag="VFN42_11725"
     CDS             814..1605
                     /locus_tag="VFN42_11725"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HET8997327.1"
                     /translation="MSDDISIPSPTYRVPRHRGADPATRRLALIAAGLGGALVVVVGG
                     WSMLGGGHHGVPVIEPQSGPTRIKPANPGGLQVQGLGNDIFSGGSDSSVDKLAPPPEV
                     PNPQALRAPPPKPVVQAAQPAVLPPPAAPAAAATPVQAPPPARAVAADHAPSMAAADH
                     PLAKPPVQPAIAHDKPVARVGGGTTMVQLAALSTEAAAKDEWRNLSRRIPGLLSGHQP
                     VFSRVDHAGHQFWRVRTGGFADQAAARGFCQRVHAKGLACSVADF"
     gene            1696..2676
                     /gene="nagZ"
                     /locus_tag="VFN42_11730"
     CDS             1696..2676
                     /gene="nagZ"
                     /locus_tag="VFN42_11730"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET8997328.1"
                     /translation="MKAAIVGVAGPRLLPAEAELFRAHPPAGVILFARNVQNPGQLAA
                     LRRAIPGDALLMVDQEGGRVARLRPPHWRAHPPAAAHGRLYAGEHRAGLRAAWLTGAL
                     IGADCRQAGFDVVAAPVLDLAHPGASDVIGDRAFGSDPSIVALLARAVADGLLAAGIQ
                     PVGKHAPGHGRALVDSHLTLPRVHAADLRADLLPFALLAHLPWLMTAHIVYAAWDPHQ
                     PATLSPAVVQGIIRGRLGFGGVLVSDDLAMGAVSGSPAERAVQALAAGCDLALYCAGD
                     LERTAELLAAVPPLSAAASARLAAARSLAESRRLPLDPAALAAERDRLFA"
     gene            2673..3425
                     /locus_tag="VFN42_11735"
     CDS             2673..3425
                     /locus_tag="VFN42_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET8997329.1"
                     /translation="MNGFVLQIVLAVAAAVPAIILHELAHGYAALALGDTTAREAGRL
                     SLNPLRHVDRFGTILLPGFLLISQLLVPPHAVRFMFGWAKPVPVAAWKFRDPRRGMAV
                     VAIAGPLMNFFLAWIGALLLPLPHMLLPRNIDPLDVALAQHPVLGTFLVYFILFNLVL
                     GLFNLLPIPPLDGGRIAVGLLPLNLARAWARMERAGILVVILVVFLLPYMLREFGIYF
                     DPFAAALNFVLPWAFHLLFLLSGHGGSGVVHV"
     gene            3418..4206
                     /locus_tag="VFN42_11740"
     CDS             3418..4206
                     /locus_tag="VFN42_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HET8997330.1"
                     /translation="MSETSDNADAPDSLVLHLEGFDGPLDLLLDLARAQKVDLAKISI
                     LALVEQYLTVIEKARRIRLELAAEWLVMAAWLTWLKSRLLVPASPDDSEDAEDAAGIL
                     AARLRDLSAIRTAAAWLAKRPQLGQDVFARGAPEDHTEIDRSRFALELGALVRSYLTA
                     VRRGTKAKRYRARALTLWTVQDALRRLGALVGTVPDWTSLEQFLPETLTGPTERRAAM
                     ASTLLAGLEMARGGAVSLRQDQAFGPILVRGRVGGVEEGHERPS"
     gene            4271..>4591
                     /locus_tag="VFN42_11745"
     CDS             4271..>4591
                     /locus_tag="VFN42_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HET8997331.1"
                     /translation="MPPDEPALRLAEALVFASAAPVTPRALKQVLQPGMDADAVIEAL
                     RTRYAGSGVELVEAGGGVQFRTAADLAPLLRKVVEVPRRLPRVAMETLAIIAYHQPVT
                     RPEIE"
BASE COUNT          597 a         1596 c         1653 g          745 t
ORIGIN      
        1 caagctgaat cgcggccatg cggtactgat cgacatcctc ggcgccgacc atggcggcta
       61 cgtcaagcgc atgcaggcag cggtgcaggc gctgtccggc ggcgaggcga aattcgaagc
      121 tgtgctctgc cagatcgtgc acatcatccg cgccggccag ccggtgagaa tgtcgaagcg
      181 cgccggcagc tatgtcacgc tgcgcgacct gctggacgag gtcggcaagg acgcggtgcg
      241 cttcaccatg ctgacccgca aggccgacgc gcagatggag ttcgacctgg accaggtggt
      301 ggcgcagacg cgcgacaacc cggtgttcta cgtgcagtac gcgcatgccc gctgccgctc
      361 ggtgctgcgg gcggcggcgg agatgttcga cgccgcaacc atcagcgacg ccgcgctggc
      421 ggggcttccc ttggactcgc tggcggccga accggaactc gcgctgatcc gccggctggc
      481 cgcttggccg cgcacggtgg aagccgccgc acaggcacgc gaaccgcacc gaatcgcgtt
      541 tttcctctat gacttggcgg ctgattttca tatgctgtgg aaccgaggca aggacgacgc
      601 cacgcttcgc ttcctgcagt ccgaccggcc ggctgaaacc ctggcgcggt tggccctggt
      661 ggcggcgacg gcggcggtga tccgctccgg cctcgcagtg atgggcgtgg cgcccgtcga
      721 ggaaatgcgc tgacccgcgg cacggacgcg gcggtcccag gggaggccgt cgatctcgtc
      781 gcgggccgcg ccagccaaaa tagaggccgc accatgtccg acgacatcag catccccagc
      841 ccgacctacc gcgtgccgcg acatcgcggc gccgatccgg cgacccgccg gcttgcgctg
      901 atcgccgccg gattgggcgg cgccctggtg gtggtcgtcg gcggctggtc gatgctgggc
      961 ggcggccatc acggcgtgcc ggtgatcgag ccgcaatccg ggccgacgcg gatcaaaccg
     1021 gccaatccgg gcggcttgca ggtgcaaggc ctcggcaacg acatcttctc cggaggcagc
     1081 gacagcagcg tggacaagct ggccccgccg ccggaggtcc cgaatccgca ggcattgcgt
     1141 gcgccgccgc cgaagccggt ggtgcaggcg gcccaacccg ccgtcctgcc gccgccggcc
     1201 gcgcctgctg ccgcagccac accggtccag gcgcctccgc ccgcacgagc cgtggcagcg
     1261 gaccacgccc ccagtatggc cgccgcggac catccgctgg cgaagcctcc cgtgcagcca
     1321 gccatcgcgc atgacaagcc ggtggcgcgg gttggcggcg gaacgacgat ggtgcagctt
     1381 gccgccctct ccacggaggc cgcggcgaag gacgaatggc gcaatctgag ccggcgcatc
     1441 cctggcctgt tgtccggaca ccagccggtg ttcagccggg tggatcacgc cgggcatcag
     1501 ttctggcgtg tccgcaccgg aggctttgcc gatcaggcgg cggcgcgcgg cttctgtcag
     1561 cgcgttcacg ccaaggggct ggcctgttcc gtggcggatt tctgatccgc ccggcgcccg
     1621 agatccagac tcctccccct cccgcaaggg gagggggagg agtttgcgtg accggctgct
     1681 tggcggatcg ccaggatgaa ggcagcgatc gtcggtgttg ccggtccgcg gctgctgccc
     1741 gccgaagccg aactgtttcg cgcccatccg cccgccgggg tgatcctgtt cgcccgcaac
     1801 gtgcagaacc cggggcagct tgcggcgctg cggcgagcca tcccgggtga cgcgctgctg
     1861 atggtggacc aggaaggcgg ccgggtggcc cggctgcgcc cgccgcactg gcgcgcccat
     1921 ccgcccgccg ccgcacacgg tcgcctctac gccggcgagc accgcgccgg gctgcgtgcc
     1981 gcctggctga ctggggcgct gatcggtgcg gattgccggc aagccggttt cgatgtcgtc
     2041 gcggcgccgg tactcgatct cgcgcatccc ggcgccagcg acgtgatcgg cgaccgcgcg
     2101 ttcggcagcg atccgtccat cgtggccctg ttggcccgtg cggtggccga cggattgctg
     2161 gcggccggca ttcagccagt gggcaaacac gccccggggc atgggcgggc gctggtggac
     2221 agccatctca cgctgccccg ggtgcacgcc gccgatctcc gggccgacct gttacccttc
     2281 gccctgctgg cccatctgcc atggttgatg accgcccata tcgtgtatgc cgcctgggat
     2341 ccgcatcagc cggccacgct gtcgccggcg gtggttcagg ggatcatccg cgggcggctt
     2401 ggttttggag gagttctggt gagcgacgat ctggcaatgg gggcggtgtc cggctcgcca
     2461 gccgagcggg cggtgcaggc tctggccgct ggctgtgacc tggcgttgta ttgtgccggc
     2521 gatcttgaac gcaccgccga actgctcgct gcggttccgc cgctgtccgc cgcggcatcg
     2581 gcgcgcctgg ccgccgcacg cagcctcgcc gagtcccgcc ggctgccgtt ggacccggcc
     2641 gcgcttgccg cggaacgcga tcggctgttc gcgtgaacgg cttcgtcctt cagatcgttc
     2701 tggcggttgc cgccgcggtt ccggcgatca tcctgcatga gctggcgcac ggctatgccg
     2761 ccctggcgct gggcgacacg acggcgcggg aggccgggcg gctgtcgctg aacccgctgc
     2821 ggcacgtgga tcggttcggc accatcctgc tgccgggttt cctgctgatc tcgcaattgc
     2881 tggtgccgcc gcacgcggtg cggttcatgt tcggctgggc caagccggtg ccggtcgccg
     2941 cctggaagtt ccgcgatccc cgccggggca tggcagtggt tgctatcgcg ggcccgctga
     3001 tgaacttttt cctggcctgg atcggcgcgc tgctgctgcc gctgccgcat atgctgctgc
     3061 cgcggaacat cgatccgctg gatgtcgcat tggcccagca tccggtgctt ggcacgttcc
     3121 tggtctactt catcctgttc aacctggtgc tggggctgtt caacctgctg ccgattccgc
     3181 cgctggatgg cggccggatc gcggtgggcc tgctgccgct gaacctggcg cgggcctggg
     3241 cacggatgga gcgcgccggg atcctggtcg tgatcctggt ggtgtttctg ctgccctaca
     3301 tgctgcgcga gttcggcatc tatttcgatc ctttcgcggc ggcgctgaat ttcgtgttgc
     3361 cctgggcatt tcacctgctg ttcctgctca gcggccacgg cggctccggc gtggtgcatg
     3421 tctgaaacct cggacaacgc ggacgcgccg gacagcctgg tcctccacct ggaggggttc
     3481 gacgggccgc tcgacctgct gctggacctc gcccgggcgc agaaggtgga cctggcgaag
     3541 atctccatcc tggcgctggt cgagcagtac ctgacggtga tcgagaaagc ccgccgcatc
     3601 cggctggaac tcgccgcgga gtggctggtg atggcggcct ggctcacctg gctgaaatcc
     3661 cgccttctgg tgcccgccag tcctgacgac tcggaggacg ccgaggacgc cgccggcatc
     3721 ctggccgccc ggctgcgcga cctgtctgcc atccggaccg ccgccgcctg gcttgccaag
     3781 cgtccgcagc ttggccagga cgtgttcgcc cggggcgcgc cggaggacca taccgagatc
     3841 gaccgctccc gtttcgccct ggaactgggc gcgctggtgc gcagctacct caccgcggta
     3901 cgccgcggca ccaaggcgaa gcgctaccgc gcccgggcac tgaccctgtg gaccgtgcag
     3961 gacgcgctgc ggcggctggg agcgctggtc ggcaccgtgc cggactggac cagcctggag
     4021 cagttcctgc cggagacgct gaccggaccg accgagcgcc gggcggccat ggcgtccacc
     4081 ctgctggccg ggttggagat ggcgcggggc ggtgcggtca gcttgcggca ggaccaggca
     4141 ttcggaccca ttctggtgcg tggccgggtc ggcggggtag aggaagggca tgagcgacct
     4201 tcctgatccc ccgccggaac cagcggcgga gcccgtccag gccgaacctc cggcaccgtc
     4261 cggcgggccg atgccgccgg acgaaccggc gctgcgcctg gccgaagcgt tggtgttcgc
     4321 cagtgccgcc ccggtcacgc cgcgtgcgct gaagcaggtg ctgcagcctg gcatggacgc
     4381 cgacgcggtg atcgaggcgc tgcgcacccg ctatgccggc agcggcgtgg aactggtgga
     4441 ggccggcggc ggtgtgcaat tccgcaccgc ggcggatctg gcgccgctgc tgcgcaaggt
     4501 ggtggaggtg ccgcgccgcc tgccgcgggt ggcgatggag acgctggcga tcatcgccta
     4561 ccaccagccg gtcacccggc cggagatcga g
//
LOCUS       DASSZH010000539         3250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_950549, whole genome shotgun sequence.
ACCESSION   DASSZH010000539 DASSZH010000000
VERSION     DASSZH010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950549"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1243)
                     /gene="cysS"
                     /locus_tag="VFN42_11750"
     CDS             complement(<1..1243)
                     /gene="cysS"
                     /locus_tag="VFN42_11750"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET8997332.1"
                     /translation="MHQLYLHDSQTRRKQRFQPLEPQHVRIYVCGPTVYDLVHVGNGR
                     PAVVFDVLVRLLRRLYPHVTYVRNITDVEDKINARAQELGIPIETLTARTAADYEADM
                     AALGVKPPDVEPRATQHIGEMITLIGRLIESGHAYAAEGHVLFAVNSYPGYGHLSGRS
                     PEELLAGARVDVAPYKREAGDFVLWKPSPPELPGWDSPWGRGRPGWHIECSAMSWKHL
                     GETFDIHGGGSDLIFPHHENEVAQSLCAFPGSHFARYWVHNGMLLVGGEKMSKSLGNF
                     ITLRDALARVPGEVIRLVLLRSHYRSTADFSDDTVAEARRELDRFYRALAKYPDLHAG
                     TEVPAPVMDALCDDLNTPLALSAMHALADAALAGDFEAAQGLRAAGDLMGVLQAAPEA
                     WFRGDGADAAAIEAAIAERLAA"
     gene            complement(1335..>3250)
                     /locus_tag="VFN42_11755"
     CDS             complement(1335..>3250)
                     /locus_tag="VFN42_11755"
                     /inference="COORDINATES: protein motif:HMM:NF024795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HET8997333.1"
                     /translation="DGKKIHKGNGMSDLQQLVYGTPFSLMITDGTVAGTQSLLTVSSG
                     FPPRTMATLANGDVVFSLDTSVGYNQLWLTDGTAGGTQLLQQFTGYGGYANIGGVTTV
                     GSNFVFDYTTGQYPGYGNFLWVSDGTAAGTMEVPGDHAADLGTSATIGTGLVFRLDNG
                     LGITDGTAAGTQSLIPYSATGFTLGYGMVQLGSQALFTATSAASGRVAGSFNELWVTD
                     GTTANTQLVATLPDSPVGGTESGNISALTSLGSKALLVYTDSSGASSFWVTDGTSAGT
                     FALSSTLVPDGIGAMTANGLLFTAGGALGVSDGTVAGTQILVAQSSNFTATDVMARLG
                     NDVVFAAGTGANELWITDGTVAGTSMIEALPAGSAIASMKSDGSQVLFTYNTTLGDVL
                     NVTDGTAAGTTAITGASSPGYGLTGSASAVIGPPCFLAGTTLATGRGTRAVEHLRVGD
                     AVLTADGSLRKVIWIGHRHVDADQHPQPEAILPVVVRADAIAPGVPQRDLYLSPDHAI
                     YLNGVLIPVKYLVDGCNILPAAHLRRPVYYHVELDCHDLLLAEGLAAESFLDTGQRGM
                     FAGADEPRLLHPAMANRGNTATWEAFGYAPLVVSGPALTAARSDIALWAAQRARFPRP
                     EEYRMGSAGCGAS"
BASE COUNT          488 a         1119 c         1113 g          530 t
ORIGIN      
        1 gtgccgccag ccgctcggcg atggccgcct cgatcgctgc cgcatccgcg ccatcgccgc
       61 ggaaccatgc ctccggcgcg gcttgcagca cgcccatcag gtcgccggcg gcgcgcagcc
      121 cctgcgctgc ctcgaaatcc ccggccagcg ccgcatccgc cagggcgtgc atcgccgaca
      181 gcgccagcgg ggtgttcagg tcgtcgcaca gtgcatccat taccggcgcc ggcacttcgg
      241 tcccggcgtg cagatccggg tatttcgcca gggcccggta gaaccggtcc agctcccgcc
      301 gcgcctccgc caccgtgtcg tcggagaagt cggcggtgga gcggtagtgg gagcgcagca
      361 gcaccagccg gatcacctcg cccggcaccc gcgccagggc gtcgcgcaaa gtgatgaaat
      421 tgcccaggct cttggacatt ttctcgccgc cgaccagcag catgccgttg tgcacccagt
      481 agcgggcgaa atggcttccg gggaaggcgc acaggctctg tgcgacctcg ttctcatggt
      541 gcgggaagat caggtcgctg ccgccgccgt ggatgtcgaa ggtttccccc aggtgcttcc
      601 aggacatggc ggagcattcg atgtgccagc ccggccggcc gcggccccag gggctgtccc
      661 agcccggcag ctcgggcggg gagggcttcc acagcacgaa gtcccccgcc tcgcgcttgt
      721 acggcgccac gtccacccgg gcgccggcca gcagttcctc cgggctgcgg ccggacagat
      781 ggccgtaccc gggatagctg ttcaccgcga acagcacgtg gccctccgcc gcgtaggcgt
      841 ggccggattc gatcagccgc ccgatcagcg tgatcatttc accgatatgc tgggtggcgc
      901 gcggctccac gtccggcggt ttcacaccca gcgccgccat gtctgcttcg tagtccgcgg
      961 cggtgcgggc ggtcagcgtc tcgatcggga tgcccagttc ctgggcgcgg gcgttgatct
     1021 tgtcctccac gtcggtgatg ttccgcacat aggtgacgtg cgggtacagc cggcgcagca
     1081 ggcggaccag cacgtcgaac accaccgccg ggcggccatt gcccacgtgc accaggtcgt
     1141 acacggtcgg cccgcacacg tagatccgca catgctgggg ctcgagcggc tggaaccgct
     1201 gcttccggcg cgtctggctg tcgtgcaggt aaagctggtg catcggcgcg agatccctgg
     1261 caggcgggct ttctccccca gtccgggcag cgaatcaacg cgcgcggcga acaagccgcc
     1321 gtgccggacg gcggtcagct cgcgccgcaa ccggccgagc ccatccggta ctcctccggc
     1381 ctgggaaacc tggcacgctg cgcggcccac agcgcgatgt cgctgcgcgc cgcggtcagc
     1441 gccggccccg acaccaccag gggggcgtaa ccgaatgcct cccaggtcgc cgtgttgcca
     1501 cggttcgcca tggcaggatg cagcaaccgg ggctcatccg cgcccgcgaa catgccgcgc
     1561 tgcccggtat cgaggaagct ctcggcggcc aggccctcgg cgagcagcag gtcgtggcag
     1621 tccagctcca catgataata caccggccgg cgaaggtgcg cggccggcag gatattgcag
     1681 ccgtccacca ggtatttgac cggaatgagc acgccgttca ggtagatcgc atgatcgggt
     1741 gacaggtaca ggtcgcgctg cggcacgccc ggggcgatcg cgtccgcccg caccacaacc
     1801 ggcaggatcg cttccggttg tggatgctga tcggcatcga cgtgccggtg gccgatccag
     1861 atcaccttgc gcagtgagcc atccgcggtc agcaccgcat cgccgacccg aagatgttcc
     1921 accgcccggg tgccgcggcc ggtggccagg gtggtgccgg cgagaaagca tggcggcccg
     1981 atcaccgcgg atgccgaacc ggtcagcccg tagccgggcg agctggcgcc ggtgatggcg
     2041 gtggtgcccg ccgccgtgcc gtcggtcacg ttcagcacgt cgcccagcgt cgtgttgtag
     2101 gtgaacagga cctgcgaacc gtcgcttttc atgctggcga tggcgctgcc ggccggcagc
     2161 gcctcgatca tgctggtgcc ggcaacggtg ccgtcggtga tccacaattc gttcgccccg
     2221 gttccggcgg cgaacacgac gtcattgccc aggcgggcca tcacatccgt cgcggtgaaa
     2281 ttgctgctct gcgccaccag gatctgcgtg ccggcgacgg tgccgtcgga gacgcccagg
     2341 gcgccgccgg cggtgaacag caggccattg gctgtcatgg cgccgatgcc gtccggcacg
     2401 agcgtgctgc tgagcgcgaa ggttcccgcc gaggttccgt cggtcaccca gaagctgctc
     2461 gcgccgctgc tgtcggtgta gacgagcagc gccttgctgc cgagcgaggt cagcgcgctg
     2521 atgttgccgc tttccgtgcc gccgaccggg ctgtccggca gcgtcgccac cagctgggtg
     2581 ttcgcggtgg tgccgtcggt cacccaaagc tcgttgaagc tgccggccac gcggccggag
     2641 gccgcgctgg tcgcggtgaa cagcgcctgg gagccaagtt gcaccatgcc gtagcccagg
     2701 gtgaagccgg tggcgctgta tgggatcagg ctctgggtgc cggccgcggt gccgtcggtg
     2761 atgccaagcc cgttgtcgag ccggaacacg aggccggtgc caattgtggc gctggtgccg
     2821 aggtccgcgg catggtcgcc cggcacctcc atggttccgg ccgccgtgcc gtcgctgacc
     2881 cacaggaaat tgccatagcc cggatattgg ccggtcgtgt aatcgaacac gaagttgctg
     2941 ccgacggtcg ttacaccgcc gatattggcg taaccgccat agccggtgaa ttgttgcagt
     3001 agctgggtgc cgcccgccgt gccgtcggtc agccacagtt gattgtagcc aacgctggtg
     3061 tccagcgaga acaccacgtc gccattggcc agggtggcca tcgtgcgggg cggaaaaccg
     3121 ctgctcacgg tcagcaggga ctgcgttccg gcgacagtcc cgtccgtgat catcagtgag
     3181 aagggcgtcc cgtaaaccag ttgctgcaaa tcggacatgc cgttcccctt gtgtatcttc
     3241 tttccgtcag
//
LOCUS       DASSZH010000540         3088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1649232, whole genome shotgun sequence.
ACCESSION   DASSZH010000540 DASSZH010000000
VERSION     DASSZH010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649232"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..788
                     /gene="phaR"
                     /locus_tag="VFN42_11760"
     CDS             66..788
                     /gene="phaR"
                     /locus_tag="VFN42_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HET8997334.1"
                     /translation="MSQPLQNDPPRPPDAAPGDQPPVVVKKYANRRLYNTESSSYITL
                     DSLAEMVRVGRDFVVYDAKTGEDITRSVLTQIIVEEESKGRAMLPTGFLRQLIGFYGD
                     QMQTLVPRYLEQAMASFARQQEQMRVAMQQTVGNFFPFGNIEEVGRQNMAMMERAMQL
                     FAPFAYRDSETNEPRATNEPHATNGSSVQDELRSLRAEVEFLRAQLAKAQAVGESETK
                     TPPVQIKAAERGSKSTRSAQSD"
     gene            934..1437
                     /locus_tag="VFN42_11765"
     CDS             934..1437
                     /locus_tag="VFN42_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET8997335.1"
                     /translation="MPETSEHTAAPGLALPEPPVTVLGPVGPDDADVEVKPSPRANAA
                     DHHVGARIRERRIMLGLSQQQLAQMIGVTYQQAHKYERGLNRISAGRLYEIAQVLGVP
                     VSWFFDGLARTEQPAEMTQRQRMCLELARNFAAIDNEKHQEALSQMARALASQSAHGS
                     NGEGGRL"
     gene            complement(1616..2332)
                     /locus_tag="VFN42_11770"
     CDS             complement(1616..2332)
                     /locus_tag="VFN42_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003620373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8997336.1"
                     /translation="MTGERPILIVDDDQALRSMLAEQLAVDGEFTASGVETAGEAEAK
                     MTARESRFDAIILDVGLPDGDGRDLCAKLRRNGIKVPIIMLTGSDEESDVVRGLDSGA
                     NDYIAKPFRMAELLARLRAQLRIFENSEDAVFTIGPYTFRPAAKLLQEPARNRRIRLT
                     EKEAAILKFLYRAGTRPVARQVLLNEVWGYNAAVTTHTLETHIYRLRQKIEPDPSNAR
                     LLVTEGGGYRLDPEGIRPAA"
     gene            2616..>3088
                     /locus_tag="VFN42_11775"
     CDS             2616..>3088
                     /locus_tag="VFN42_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HET8997337.1"
                     /translation="MTQVKHRGNAPPRPPASAAPDPVALRLRGVHRAIADLRRGTPVL
                     LEGRETLVLLAAETAGAAGLAEMAAFAAGVPLLLLAPVRAAAVLRRPVTQEAAVALPL
                     SRALLDPARLRSLADPTAEDMLPADPPAPVPPPPGAEAALALGKLARLLPAMLAVP"
BASE COUNT          531 a         1037 c         1019 g          501 t
ORIGIN      
        1 ggccctgttt tgtttattgc accgcggcat gctaccgttg ggacgaagat cgcgcgaggg
       61 cagacatgtc gcagccgcta cagaacgatc ccccccggcc gcccgatgcc gcaccgggcg
      121 accagccccc cgtggtggtc aagaaatacg ccaaccgacg cctctacaac accgagagtt
      181 ccagctatat cacgctcgat tcgctggctg aaatggtgcg tgtcgggcgc gatttcgtcg
      241 tctacgacgc caagaccggc gaggacatca cccgcagcgt gctcacccag atcatcgtgg
      301 aggaagagag caaggggcgc gcgatgctgc ccaccggctt cctgcgccag ttgatcgggt
      361 tctacggtga ccagatgcaa acactcgtgc cgcgctacct ggagcaggcc atggccagct
      421 tcgcgcggca gcaggaacag atgcgggttg cgatgcaaca gacggtgggc aatttctttc
      481 catttggcaa catagaagag gtcggacgcc agaacatggc catgatggag cgggccatgc
      541 agctgttcgc tccatttgct taccgcgact ccgaaacgaa tgaaccgcgt gctacgaatg
      601 aaccgcacgc tacgaacggg tccagcgtgc aggatgagct caggtcgctg cgcgcggagg
      661 tcgaattcct gcgggcgcag ctggcgaagg cgcaagctgt gggggagagt gaaacaaaaa
      721 cgccccccgt acaaataaag gcggcagagc gcggcagtaa atcgacccga agcgctcaaa
      781 gcgactgaag ccgatatcgt tcaaccagcg gagagcgggc aaagacaccg ccacgtcaag
      841 tctcaaagta gcattcgcgc gaggcgggaa tctttgagta tccctggtgc tttgcctgtg
      901 gcagtggcta taggcgaagg agggacactc accatgcccg agacgagcga acacactgcc
      961 gcgcccgggc tcgctttgcc cgagccgccg gtcaccgtac ttggtccggt ggggccggac
     1021 gatgctgacg tcgaagtcaa gccgtcgccg cgcgccaatg ccgccgacca tcatgtcggc
     1081 gcgcgcatcc gcgaacgccg catcatgctg ggcctgtcgc agcaacaact cgcccagatg
     1141 atcggcgtca cctaccagca ggcgcacaaa tacgagcgcg gtctcaaccg catttccgcc
     1201 ggccggctgt atgagatcgc gcaggtgctc ggcgtgccgg tctcctggtt cttcgacgga
     1261 cttgcgcgca ccgaacagcc ggccgagatg acccagcggc agcgcatgtg cctggaactg
     1321 gcgcgcaact ttgccgccat cgacaacgag aagcaccagg aagcgctcag ccagatggcc
     1381 cgtgccctgg ccagccagtc ggcccatggc tccaacggcg agggcggacg gctctagccg
     1441 ccccgcacgg ccttatcccg cgccgcgtgc caccctctcc cagccacggg agaggggcgc
     1501 agggtatccg ctccaaatag tgaaagaaac ttgcctgaac gtttccctct cccgttgccg
     1561 ggagagggtc gggattctgg ctagatcaat cctggccggc tcaatcctgg ccggctcagg
     1621 cggcggggcg gatgccctcg gggtcgagtc ggtagccgcc gccttcggtc accagcaggc
     1681 gggcattcga cgggtccggc tcgatcttct gccgcagccg gtagatatgc gtctccagcg
     1741 tgtgcgtggt gacggcggcg ttgtagcccc agacctcgtt cagcagcacc tgccgtgcca
     1801 ccggccgcgt gccggcgcga tacaggaact tcaggatcgc cgcttccttc tcggtcagcc
     1861 ggatgcggcg gttacgagcc ggttcctgca gcagtttcgc cgccggccgg aacgtatagg
     1921 ggccgatggt gaacaccgca tcctcgctgt tctcgaagat gcgcaactgc gcccgcaggc
     1981 gggcgagcag ctcggccatg cggaacggct tggcgatgta gtcgttggcg cccgagtcga
     2041 ggccgcgcac cacgtcgctc tcctcgtccg agcccgtcag catgatgatc ggcaccttga
     2101 tgccgttgcg gcgcagcttg gcgcacagat cgcgcccgtc gccgtcgggc aggccgacat
     2161 cgaggatgat ggcgtcgaag cgcgattcgc gggcggtcat cttggcctcg gcctcgcccg
     2221 ccgtctccac cccgcttgcc gtgaactcgc cgtcgacagc cagttgctcg gcgagcatcg
     2281 agcggagtgc ctgatcgtcg tccacaatga gaatcggtcg ctcgcccgtc attcccctct
     2341 accctccatt ggcgatagcg cggccgagtg ccgggcttcg cggtttcaca atcgccgatc
     2401 tgggtgaaac cgttgcaacc cggatcggta gcgtatccca acgcgcgact tgctcgtggt
     2461 tacattcttg cgcaggttca agccgcacac atctccgtga atcgtgggcg gcaacgtgac
     2521 cgtgaggccg agatagatca gggcaccccg gctttcgaca cccttgcaga ggtcgcctct
     2581 gccgggaatg ttgggcaaca ccggaccagg accccatgac ccaagtcaag catcgcggca
     2641 atgccccgcc ccgcccgccg gcatcagccg cacccgatcc ggtggccctg cggctgcgcg
     2701 gcgtgcaccg cgccatcgcc gatctgcgcc gcggcacgcc ggtgttgctg gaagggcggg
     2761 agacgctggt gctgctggcg gcggaaaccg ccggggcggc gggattggcg gaaatggccg
     2821 cgttcgccgc cggtgtgccg ctgctgctgc tggctcccgt ccgggcggcg gcggtgctgc
     2881 gccggccggt gacgcaggaa gcggcggtgg ccctgccgct gtcgcgggcg ctgctcgacc
     2941 cggcgcggct gcgcagcctc gccgatccga cggcggagga tatgttgccc gccgacccgc
     3001 ccgcgccggt gccgccgccc cccggcgcgg aggcggcgct ggcgctgggc aagctcgcgc
     3061 ggctgctgcc ggccatgctg gcggtgcc
//
LOCUS       DASSZH010000541         4273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_796860, whole genome shotgun sequence.
ACCESSION   DASSZH010000541 DASSZH010000000
VERSION     DASSZH010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796860"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VFN42_11780"
     CDS             <1..813
                     /locus_tag="VFN42_11780"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET8997338.1"
                     /translation="QRLPPSPRRRLGFAAIASVVLHAAVLALLLVGFAHRQRMVAAAD
                     TPAVVELVMSPPGNSKTSAPATAPPHAMQARPAAKPVPPVPPRPPQPVPAPVPPAAAP
                     PPPPPDGSTVQALVAPEPAAAQPEAAQAAPAPEQPQPAKPAPATAPAPPAPEATPALQ
                     LSLGGIASDTNALVTGRMLLPPGADPKFRNRKPNYPQQAALRGEEGTVVLMIHISPEG
                     LVSGVDILQSTGYPDLDRSARDAVMSWHFLPAIQDGQPVPFQMRMRIQFALF"
     gene            826..1377
                     /locus_tag="VFN42_11785"
     CDS             826..1377
                     /locus_tag="VFN42_11785"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HET8997339.1"
                     /translation="MVRIDVVVTRGGDGGETSLGDGTRVRKDSPVIAAIGAVDEANAS
                     IGVLRLCTRDDAATDAMLARVQNDLFDLGADLCVPGEGGNRLRLTDAPVLRLEAEVAA
                     MNAALPPLRSFILPGGSAGAAHAHLARTVVRRAERAVIAVDGLNPMLKRYLNRLSDHL
                     FVLARMLNDRGAGDVLWEPGANR"
     gene            1671..1964
                     /locus_tag="VFN42_11790"
     CDS             1671..1964
                     /locus_tag="VFN42_11790"
                     /inference="COORDINATES: protein motif:HMM:NF023174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HET8997340.1"
                     /translation="MLFLVVSEPRPEPPSSVAASRQRFWTWAAPLQARGELRAIYAKV
                     GRGAVALFDVPSNEVLHRRLNEWADIIPARFDVHALIDPDAAQAFLGEQRAAV"
     gene            2037..3026
                     /gene="lhpH"
                     /locus_tag="VFN42_11795"
     CDS             2037..3026
                     /gene="lhpH"
                     /locus_tag="VFN42_11795"
                     /EC_number="4.2.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF045511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050346 - trans-L-3-hydroxyproline
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-3-hydroxy-L-proline dehydratase"
                     /protein_id="HET8997341.1"
                     /translation="MTTVEMHTAGEPFRIVTSGFPRAPGARIVDRRAWLRDNADHLRR
                     ALIMEPRGHADMYAGILTDPVSPGADLGVIFAHNEGYSDHCGHGTIALATAAVQLGWV
                     ARSVPETRVGIDAPCGFVQAFVAWDGARAGAVRFVNVPSFLFARDIVVETPSFGRVVG
                     DIAYGGAFYVYTSGAACGLSIIEADVERLVRFGAEVKAAANARYPVVHPEIPEINHIY
                     GTIIDGPPRNPGSTQSNCCVFADRQVDRSPTGSGTAGRLAQLHARGRLTAGDVLVNDS
                     IIGSTFSGRVLREARVGTHQAVIPEISGQAWLCGLATWMIDQHDPQRQGFLVR"
     gene            complement(3371..3817)
                     /locus_tag="VFN42_11800"
     CDS             complement(3371..3817)
                     /locus_tag="VFN42_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006557059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HET8997342.1"
                     /translation="MPEPFVSPEEASAADAIAAIRDELEVFDDWMDRYQFIIELGRKL
                     PAFPEQWMDDAHRVPGCQSRVWMEAACQDGKLWFTAVSDAAIVSGLLALLLRVYSGRS
                     AEEVLATDPVFLKDLGLLEALSTNRGNGIAAMARKVREVAALQKTA"
     gene            3958..>4273
                     /locus_tag="VFN42_11805"
     CDS             3958..>4273
                     /locus_tag="VFN42_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine aminotransferase"
                     /protein_id="HET8997343.1"
                     /translation="MASASKPDMHPANPNFSSGPCAKRPGFTLEALSGAFLGRSHRAA
                     RPKARLAEIITRSRAILGMPDDWRLGIVPASDTGAVELALWSLLGPRPVDILAFESFG
                     EGW"
BASE COUNT          635 a         1450 c         1514 g          674 t
ORIGIN      
        1 cagcggctgc ccccgtcgcc ccggcgccgg ctgggctttg cggcgatcgc gtcggtggtg
       61 ctgcacgcag cggtgctggc gctcttgctg gtcggcttcg cccaccggca gcgcatggtg
      121 gccgcggccg ataccccggc ggtcgtcgaa ctggtgatgt cgccgcccgg caacagcaag
      181 accagcgcgc cggcaaccgc gccgccgcat gcgatgcaag ccaggcccgc agccaagccg
      241 gtcccgccgg tcccgcccag gcctccccaa ccggtacccg ctcccgtgcc gccggcagcc
      301 gcccccccgc cgcccccgcc ggatggcagc acggtccagg cgctggtcgc gccggagccc
      361 gccgccgcac agccggaagc cgcccaggcg gcaccggcgc cggaacagcc gcagcctgcc
      421 aagcctgcgc cggccaccgc cccggcgccg cctgcgcccg aggccacacc cgccttgcag
      481 ctcagcctgg gcggcatcgc cagcgacacc aatgcgctgg tgaccggcag gatgctgctg
      541 ccccccggcg ccgatccgaa attccgcaac cgcaagccga attacccgca gcaggcggcg
      601 ttgcgcgggg aggaaggcac ggtggtgctg atgatccata tctcgccgga ggggctggtg
      661 agcggtgtcg acatcctgca aagcacgggg tacccggatc tcgaccgctc ggcgcgggat
      721 gcggtgatga gctggcactt cctgccggcg atccaggacg gccagcccgt gcccttccag
      781 atgcggatgc ggattcagtt tgcgctgttc tgaacggggc ccctgatggt gcggatcgat
      841 gtggtggtga cgcgcggcgg cgatggcgga gagacctcgc tgggcgacgg cacgcgggtg
      901 cgcaaggact cgccggtgat cgcggcgatc ggcgcggtgg atgaggcgaa tgcgtcgatc
      961 ggcgtgctgc ggctgtgcac gcgggacgac gccgcgaccg atgccatgct ggcgcgggtg
     1021 cagaacgacc tgttcgacct gggtgccgat ctgtgcgtgc cgggcgaggg gggcaaccgc
     1081 ttgcggctga ccgacgcgcc ggtgctgcgg ttggaggcgg aggtcgcggc gatgaatgcg
     1141 gcgctgccgc cgctgcgcag cttcatcctg ccgggcggca gtgccggggc ggcgcacgcg
     1201 catctggccc gcaccgtggt gcggcgcgcc gaacgcgcgg tgatcgcggt ggacgggctg
     1261 aatccgatgc tgaagcgcta cctcaaccgg ctgtccgacc acttgttcgt gctggcccgg
     1321 atgttgaacg accgcggggc cggcgatgtg ttgtgggagc cgggcgcgaa ccgctgacct
     1381 gctgcgggag gaccgcccgc gcgcggctgc acgcgggcgg catggtcaga tcacgtggaa
     1441 gaggtagagc aggatgatga ccgggatcgg aatccccagt acccaaagca gaatgccgcg
     1501 catgggcccc acctggttgt tgccgtcgca accaaacagt ccagcgggtt gatcgttcct
     1561 gcggcggcca tcacgtttcg gtgccatacc ggcaggcccg cggcggcaat gcctctggtt
     1621 cggcgtcagc gatcggcgca aaaaggggcg gataagagag tggaggaaac atgttgttcc
     1681 tggtggtcag tgagccgcga ccggagccgc ccagcagcgt cgcggcatcg cggcagcggt
     1741 tctggacctg ggcggcgcca ttgcaggcgc gcggcgaact gcgcgcgatc tacgccaagg
     1801 tggggcgtgg cgcggtggcg ttgttcgacg tgccctccaa cgaggtgctg caccgccggc
     1861 tcaacgaatg ggccgacatc atcccggcgc gcttcgacgt gcatgcgctg atcgatccgg
     1921 acgccgcgca ggcgttcctg ggcgagcagc gggccgcggt ctgagcacga caagatcgcc
     1981 cgcgcctgtg gcgcggtggc ggaaaaacgg cggaggcgga tctgctcgac cacaccatca
     2041 ccaccgtcga gatgcacacg gcaggcgagc cgtttcgcat cgtgaccagc ggttttccgc
     2101 gcgcaccagg ggcacgcatc gtggaccggc gcgcctggtt gcgggacaat gccgatcatc
     2161 tgcgccgcgc attgatcatg gagccgcgcg gtcacgccga catgtatgcc ggcattctca
     2221 ccgatccggt ttcgcccggc gccgatcttg gcgtgatctt cgcgcacaac gagggctata
     2281 gcgaccattg cggccatggc accatcgcgc tcgccaccgc ggcggtgcaa ctgggctggg
     2341 tcgcgcgcag cgtgccggaa acgcgcgtcg gcatcgacgc accctgcggt ttcgtgcaag
     2401 ccttcgtggc atgggacggg gcgcgcgcgg gtgccgtccg gttcgtcaat gtgccatcct
     2461 tcctgttcgc gcgggacatc gtggtggaga cgcccagctt cgggcgtgtg gtgggcgaca
     2521 tcgcctatgg cggcgccttc tacgtctaca catccggcgc ggcgtgtggc ctgtcgatca
     2581 tcgaagccga tgtggagcgg ctggtgcggt tcggcgcgga agtgaaggcc gcggcgaatg
     2641 cgcgctatcc ggtggtgcat ccggagatcc cggagatcaa ccacatctac ggcacgatca
     2701 tcgacggccc gccgcgcaat ccgggttcga cacagagcaa ttgctgcgtg ttcgccgatc
     2761 gtcaggtgga tcgctcgccg acgggatcgg gcaccgcggg gcggctcgcc cagttgcatg
     2821 cgcgcgggcg cctgacagcc ggcgacgtgc tggtgaatga ctcgatcatt ggctcgacgt
     2881 tttccggccg tgtgctgcgc gaggcgcgcg tcggcacgca ccaggcggtg atcccggaga
     2941 tttccggcca ggcctggcta tgcggccttg ccacttggat gatcgatcag cacgacccgc
     3001 agcgccaggg tttcctggtg cgataacgcc catcgcggcc ggcgagaagc ggccagcacg
     3061 ctggaatccg acgtggcggt tttgctctgc ggactctgcg gtcccctgcg tctctgcgcg
     3121 gaatgcggtg ctggagcaat ctccggcgcc acccgcgtgc gtcgaacgcg cgatccatgt
     3181 cacatgctcc aggacagggg gtaccgcggc cccgtattcc gcgcagagac gcagaggatc
     3241 gcagagttcg cagggcgcct tcacgcgaag gtttcgaaca acttcgcttt gggataaatg
     3301 cctttgcggc cgagtgtatg acggctcttg ccggcgttcc gggttcagcg ctgcagcgcg
     3361 cctggcgggg tcacgccgtc ttctgcagcg ccgccacttc ccgcaccttg cgcgccatcg
     3421 cggcgatgcc gttgccgcgg ttggtggaga gcgcctccag caggccgagg tccttcagga
     3481 acaccgggtc ggtcgccagc acctcctccg ccgagcgccc ggaatacacc cgcagcagca
     3541 gcgccagcag gccggagacg atcgccgcat ccgacaccgc ggtgaaccac agtttgccgt
     3601 cctggcacgc cgcttccatc cacacccggc tctggcagcc gggcacgcga tgcgcatcgt
     3661 ccatccattg ttccgggaaa gcaggcagct tgcggcccag ttcgatgatg aactggtagc
     3721 ggtccatcca gtcatcgaac acctccaact cgtcgcggat cgcggcgatg gcgtcggcgg
     3781 cggaggcttc ctcggggctt acgaaaggtt ccggcatgca ccgcatgtgc gaggcggggc
     3841 agggcgcgtc aagacggcgg cacttggttg ttgacgcttc gatacgccgg gattacatgc
     3901 cgccccgggt cacgcccccc caccggggcg cttctcttct gaaagggaga gctatcgatg
     3961 gcaagcgcca gcaagccgga catgcatccg gccaatccca atttctcctc cggcccctgc
     4021 gccaagcgtc ccggcttcac gctggaagcg ctgtccggcg cgttcctcgg ccgcagccac
     4081 cgtgctgcca ggcccaaggc acggctggcc gagatcatca cccggtcgcg cgcgatcctg
     4141 ggcatgccgg acgactggcg gctgggcatc gtccctgcct ccgacacggg cgcggtggag
     4201 ttggcgctgt ggtcgctgct cggcccgcgc ccggtcgata tcctcgcctt tgagagcttc
     4261 ggcgagggct ggg
//
LOCUS       DASSZH010000542         3121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_865076, whole genome shotgun sequence.
ACCESSION   DASSZH010000542 DASSZH010000000
VERSION     DASSZH010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865076"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="VFN42_11810"
     CDS             <1..980
                     /locus_tag="VFN42_11810"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8997344.1"
                     /translation="LRSSFAIGFFTVLMGLSQTVWQFFGARALMGAFAGFSASAIALV
                     ASQVPEERLGYALGWLSTGQLVGGLVGPVLGGFLSDLSGSYRVPFFCTAVICFVAMGL
                     AWAFVREHFTPPAQAGRGRSMLSALRLLTTSGGLLSLFFVLLLAQFGVRTVQPVVTLF
                     VQQLVGNTPSLATLAGLAFSVTGVADLIASPFLGKRSDVIGYRRVLLICLLGAGIMSI
                     PQAFAHSYWAFVAERFGVGCFVGGILPTANALVGRLVPRAQRGTVYGLTASATFLGNS
                     MGPLTGGGVAAVLGLRWVFLVTAGLLLANLVWVYFTVPELPEQVSGSSR"
     gene            complement(995..1357)
                     /locus_tag="VFN42_11815"
     CDS             complement(995..1357)
                     /locus_tag="VFN42_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997345.1"
                     /translation="MRHPLPLLLLLLAGCQASPLTLTTVVGGVTVGSVAVLHRSPFDA
                     AWSLLTGRDCSVVRLDQGKTWCRMPEPPPDPPPYCTRSLGTVDCWADPAGKPPEVADG
                     PRSLTPAQEADRIRSWPF"
     gene            1476..2828
                     /locus_tag="VFN42_11820"
     CDS             1476..2828
                     /locus_tag="VFN42_11820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008892251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET8997346.1"
                     /translation="MAQAYDLVVVGTGVAASVVAGRVRKAGWTVAVVDSRPFGGTCVL
                     RGCDPKKVLLAGAEAVDRARRLSQHGVAGDTRIDWPALMAFKRGFTDPVPQRRERGFA
                     EQGIDALHGAARFTGPDRIAVGEQTLQARHILLATGARPVQLSFPGAEHLTTSDAFME
                     LPDLPRRIVMVGGGYIAAEFSHLAARAGAQVTVVQRAGQMLTPFDPELVGWLMARFRD
                     LGIDVRTGTTVARVERNQQGGFVVRTQGGQGEGTIEADLVVHAAGRAPDLAPLDLDAG
                     GVARDEHGRLRLNAYLQSTSNPRVYAAGDAAGAGPPLTPVASLDGEAVAGNLLHGNRD
                     VPDYNGVPSVAFTIPPIAAVGLSEQAARAQGLQFSVSCSRADDWFTAKRLGESVYGYK
                     VLIEQGSDRILGAHLVGPHAEETINLFALAIRHRLPASVLRDTVYAYPTAGSDVSSML
                     "
BASE COUNT          401 a         1027 c         1172 g          521 t
ORIGIN      
        1 tgctgcgctc cagcttcgcc atcggcttct tcaccgtgct gatggggctg tcgcagaccg
       61 tctggcagtt cttcggtgcc cgcgcgctga tgggtgcctt cgccggcttc tccgcctccg
      121 ccatcgcgct ggtggcgagc caggtgccgg aggagcgact cggctacgcg ctgggctggc
      181 tcagcaccgg ccagttggtc ggcggcctgg tcggcccggt gctgggcggt ttcctgtcgg
      241 acctctccgg cagctatcgc gtgccgttct tctgcaccgc ggtgatctgc ttcgtcgcca
      301 tggggctggc ctgggctttc gtgcgcgagc acttcacccc gccggcgcag gcgggccgcg
      361 gccgctccat gctgtcggcg ctgcggctgc tgaccaccag cggtgggctg ctgtcgctgt
      421 tcttcgtgct gctgctggcg cagttcggcg tgcgtacggt gcagccggtg gtgacactgt
      481 tcgtgcagca actggtgggc aacacgccca gcctggcgac actggcgggg ctggcgttct
      541 ccgtcaccgg cgtggcggac ctgatcgcct cgcccttcct gggcaagcgc agcgacgtga
      601 tcggctaccg gcgcgtgctg ctgatctgcc tgctgggcgc gggaatcatg agcatcccgc
      661 aggctttcgc gcacagctat tgggccttcg tggccgaacg cttcggtgtg ggctgctttg
      721 tcggcggcat cctgccgacg gcgaacgcgc tggtcgggcg tctggtgccg cgggcgcagc
      781 gcggcacggt gtacgggctg accgcctccg ccaccttcct gggcaactcg atggggccgc
      841 tgactggtgg cggggtggcg gcggtgctgg ggctgcgctg ggtgttcctg gtcaccgccg
      901 ggctgctgct ggccaacctg gtgtgggtgt atttcaccgt gccggagttg ccggaacagg
      961 tgtccggctc gtcgcgctga gccggcggca cgggtcagaa cggccaggac cggatgcggt
     1021 cggcctcctg cgccggggtg aggctgcgcg ggccgtccgc cacttccggc ggcttgccgg
     1081 ccggatcggc ccagcaatcc accgtgccca ggctgcgcgt gcagtagggc ggcgggtccg
     1141 gtggcggttc cggcatgcgg caccaggttt tgccctgatc cagccgcacc accgagcagt
     1201 cgcgcccggt gagcagcgac cacgccgcgt cgaacgggct gcggtgcaat accgcgacgc
     1261 tgcccaccgt gacgccgccc acgacggtgg tcagcgtcag cgggctggcc tggcagccgg
     1321 ccagcaggag cagcagcagt ggcaacgggt ggcgcatgcc cggcaccctg cgccgcaaag
     1381 gtgaacaagc cgtttcccgc ccatgcgcgg ggtcgtgaag cgcaggccgg ccgattctgc
     1441 caatgtctcc ccagttggca gcgggaggtg aagccatggc gcaagcctat gatctggtcg
     1501 tcgtcggcac cggcgtagcc gccagcgtcg tcgccggccg ggtgcggaag gccggctgga
     1561 cggtcgcggt cgtggacagc cggccgttcg gcggcacctg cgtgctgcgc ggctgcgatc
     1621 cgaagaaggt gctgcttgcc ggcgccgagg cggtggatcg ggcgcgccgc ctctcgcagc
     1681 acggcgttgc cggagacacg cggatcgact ggccggcgct gatggcgttc aagcgcggct
     1741 ttaccgaccc ggtgccgcag cggcgcgaac gcggcttcgc ggagcaaggc atcgacgcgc
     1801 tgcacggcgc cgcgcgcttc accggccccg accgcatcgc agttggcgag cagaccctgc
     1861 aggctcggca catcctgctg gcaaccggcg cccgtccggt gcagctgtcc tttcccggcg
     1921 cggagcatct gaccaccagc gacgcgttca tggaactgcc ggatctgcca cgccgcatcg
     1981 tgatggtcgg tggcggatac attgccgccg agttctccca ccttgccgcg cgggccggcg
     2041 cgcaggtgac ggtagtgcaa cgggcaggtc agatgctgac gccgttcgat ccggagctgg
     2101 tcgggtggct gatggcgcgc ttccgcgact taggcatcga tgtccgcacc ggaaccacgg
     2161 ttgccagggt ggaacgcaac cagcagggcg gcttcgtggt gcgcacccag ggtggccagg
     2221 gcgaggggac gattgaagcc gacctggtgg tgcatgcggc gggccgcgca ccggatctcg
     2281 cgccgctgga cctggacgcc gggggcgtgg cgcgcgacga acacggacgg ctgcggctga
     2341 acgcgtatct gcaaagcacg tccaatccgc gcgtctatgc cgccggggac gccgcggggg
     2401 ccggcccgcc gctgacgccg gtcgccagcc tggacgggga ggcggttgcc gggaacctgc
     2461 tgcacggcaa tcgtgacgtt cccgactata acggtgtgcc aagcgtcgcc ttcaccattc
     2521 cgccgatcgc cgcggtgggg ctgagcgagc aggcggcgcg cgcgcagggc ttgcagttca
     2581 gcgtttcctg ttcgcgggcg gacgactggt tcaccgcgaa gcggctgggc gagagcgtgt
     2641 acggctacaa ggtgctgatc gagcagggct ccgatcggat cctgggggcg catctggtcg
     2701 ggccgcatgc cgaggagacg atcaacctgt tcgcgctggc gatccgtcac cggttgccgg
     2761 cctcggtgct gcgcgacacg gtgtatgcgt atccgaccgc gggatcggat gtgagttcca
     2821 tgctgtagcg cgaagcggtg gggcgaatca cgatggcgga gcaggccggg aggccggccc
     2881 cgcggatcag gcggcttgca ccagcggcgg cgggccggtc ggcggggctc tcgccgagcg
     2941 tgccggagcg cgtttgtacc gcctggcaac ggcgatgccg ctgccgagtt ctgtctgacc
     3001 cacatcaaga gcggtgcgga aggagaaggg gttctatgcc tctgcgccga ccatgctgtt
     3061 gaacagcgcc atcgccgttg acgatttctg ggaggatcgt cgtcgaaaag tgagccgccg
     3121 a
//
LOCUS       DASSZH010000543         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1188910, whole genome shotgun sequence.
ACCESSION   DASSZH010000543 DASSZH010000000
VERSION     DASSZH010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188910"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFN42_11825"
     CDS             <1..201
                     /locus_tag="VFN42_11825"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223295.1"
                     /note="NADPH-dependent; catalyzes the reduction of
                     7-cyano-7-deazaguanine to 7-aminomethyl-7-deazaguanine in
                     queuosine biosynthesis; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HET8997347.1"
                     /translation="HGAFHEACTMQIADRLTETLQPVWLRIGGYWYPRGGMPIDVFWQ
                     SGTPPEGAWIPAQDVAPYRGRG"
     gene            complement(211..1389)
                     /locus_tag="VFN42_11830"
     CDS             complement(211..1389)
                     /locus_tag="VFN42_11830"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET8997348.1"
                     /translation="MPQPSGPLAGIRVIDITTVVLGPYAAQILGDMGADVIKVESPRG
                     DDTRWIGPSRSEGMGAYFANLNRNKRSIVLDLKQPAHQQALLRLAATADVLVHNMRPG
                     AAQRLGMDYASIAACNPRIVYASASGFRKGSSRQEAPSFDDLTQGISGLAALNAGPDG
                     APRYVPTVLVDKLTGHMLASMVAMALVHRERTGQGQEVHVPMLETTLGFLLAEHMWWA
                     TFGEAERGIGYPRMLSPNRRPYPTKDGFISVIASSDAQYMRLFQALGRPELADDPRFN
                     SLGARARNIDLLLSMLTEGLRSRNTEEWLAVLTAADIPCGRVNALQDLLLDEYLQETG
                     FFQQMQHPTEGAVTVPSIAATFSASPASVQRLWPKLGEHTAEILREIGYDEAEIAQVT
                     "
     gene            1518..>2086
                     /locus_tag="VFN42_11835"
     CDS             1518..>2086
                     /locus_tag="VFN42_11835"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HET8997349.1"
                     /translation="MDVSVIAQTRAFNRTAAERIGVLTDQFLGRARPFGETRALWEIG
                     PQGLEVRRLRARLGLDSGYASRLLRSLERQGLVTVATSREDGRVRCVRLTRRGLAEYS
                     EINRRSDAVASSYLEPLSEAQRKRLIAAMAEVEQLLRASMIVVAVEHPGTPDARHCLG
                     RYFEELNTRFESGFDAPAALAAAMHDLAAP"
BASE COUNT          334 a          678 c          702 g          372 t
ORIGIN      
        1 catggcgcgt ttcacgaggc gtgcaccatg cagatcgccg atcggttgac ggagacattg
       61 cagccggtgt ggctgcgcat cggcggctac tggtatccgc gcggcggcat gccgatcgac
      121 gtgttctggc agagcggtac cccgccggaa ggtgcctgga tcccggcgca ggacgtcgcc
      181 ccctaccgcg gccgcggctg acagctcgat tcaggtcacc tgcgcgatct cagcctcgtc
      241 ataaccgatc tcgcgcagta tctccgccgt atgctcgcca agcttcggcc agagccgttg
      301 caccgaggcc ggactggcgg agaatgtcgc ggcgatggac ggcacggtga ccgcaccttc
      361 cgtcggatgc tgcatctgct ggaagaatcc cgtctcctgg aggtactcgt cgagcagcag
      421 atcctgcagc gcattgacgc ggccacaggg aatgtcggcc gccgtcagca ccgccagcca
      481 ttcttccgtg ttgcgcgacc gcaaaccttc ggtcagcatc gacagcagca ggtcgatgtt
      541 gcgtgcccgc gcgcccaggc tgttgaagcg cggatcgtcc gccagctccg gccggccaag
      601 cgcctggaac agccgcatgt attgcgcatc gctggaggcg atcacactaa tgaatccatc
      661 cttggtcgga tatggccggc ggttcggtga caacatgcgt gggtagccga tgccgcgctc
      721 cgcttcgccg aaagtggccc accacatgtg ttccgccagc aggaagccga gcgtggtttc
      781 cagcatcggc acgtgcactt cctggccttg cccggtgcgt tcgcggtgca ccagcgccat
      841 ggcgaccatc gaggcgagca tgtggccggt cagtttgtcc accagcactg tcggcacgta
      901 gcgtggggcg ccatccggac cggcgttcag tgcggccagc ccgctgatcc cctgtgtcag
      961 gtcgtcaaac gacggcgctt cctgccggct gctgcctttg cggaagccgg aggcgctggc
     1021 gtacacgatg cgcggattgc acgcagcaat gctggcatag tccatgccaa gccgctgtgc
     1081 cgcgccggga cgcatgttgt gcacaagcac atcggccgtt gccgccagcc gcagcagcgc
     1141 ctgctgatgc gccggttgct tcaggtccag cacgatgctg cgcttgttgc ggttgaggtt
     1201 ggcaaaatag gcgcccatgc cctcgctgcg cgatggaccg atccagcgcg tatcgtcgcc
     1261 acgcggcgat tccaccttga tcacatcggc gcccatatcc cccagaattt gcgcggcata
     1321 gggaccaagc acgacggtgg tgatgtcgat gacgcgaatg ccggcgagcg ggccggaggg
     1381 ctgtggcatg ggtcgtcctc gtgaaattgc cggcggcagc ttcggcggtg gcataccgat
     1441 tggcaatgcg gcgatccacg cctgtcgctg gcgctccggt ttgacgccgt ggcatatctc
     1501 tgactaaagt cagaggcatg gatgtgtccg tcatcgcgca aacccgtgcc ttcaaccgca
     1561 ccgccgcgga gcgcatcggc gtactgactg accagtttct tggccgcgcg cgcccgtttg
     1621 gcgaaacccg cgcactttgg gagatcgggc cgcagggcct ggaagtgcga aggctgcggg
     1681 cacggctcgg gctggactcc ggctatgcca gccgcctgct gcggtcgctg gagcgccagg
     1741 gcctggtgac cgtcgccacc agccgcgagg acgggcgggt gcgctgtgtg cggctgacca
     1801 ggcgcgggct cgccgagtac tccgagatca atcggcgttc cgatgccgtg gcatccagct
     1861 acctggagcc gctgagcgag gcacaacgga agcgcctgat cgccgccatg gccgaagtgg
     1921 agcagttgct gcgggcgtcc atgatcgtgg tggcggtcga acatcctggg acgccggacg
     1981 cgcgtcactg tcttggccga tatttcgagg aactgaacac gcggttcgaa tcgggcttcg
     2041 atgcgccggc ggcgcttgcc gcggcgatgc acgaccttgc cgcgcc
//
LOCUS       DASSZH010000544         5851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1683679, whole genome shotgun sequence.
ACCESSION   DASSZH010000544 DASSZH010000000
VERSION     DASSZH010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683679"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VFN42_11840"
     CDS             <1..619
                     /locus_tag="VFN42_11840"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET8997350.1"
                     /translation="YHGRAAHLVQAMMIEAVTGQDYRDVIRQRVIEPLGLGGDIFVGV
                     PPQMQHRCADTYAPEPRDNSAEFRAAGLPHGGGFATARGMAAFYQMLCAGGRLASVRL
                     FSPRLLAYVARNHTGERGDVQMDGIPMHRGLGPHVRGESDRIRGLGTIGAPQTFGHGG
                     VGSSYSWADPTSGVSFTYLSNFVVPDPWHSARLDRVSNLVHAAID"
     gene            complement(750..1838)
                     /locus_tag="VFN42_11845"
     CDS             complement(750..1838)
                     /locus_tag="VFN42_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997351.1"
                     /translation="MRHLRIFCATALTALLAVGGTVSARAADCAPAAGVKFTCGVTNV
                     EDFAPVPGTHWVIGSDLAAAGKQGTLYLFDTRTGKASPVEPQEIAIRPDKMRYKDCPG
                     PLDMNIFGPHGLDLTQGKGPHRTLYAVNHGGRESVEVFAVDLTKARPHFTWVGCVVAP
                     QHFWPDAVASLPDGGIIVTSLWDPTDPHRMDKLANGQPVGALDEWQAGKGWSEVPGSA
                     GMSGPNGVIASPNGKEVYVALWSGHAVTRISRGTMPVRKASVPTGILTDNLRWSPDGR
                     SIFVGGQDASVKQVIACFESSAVNCNVPFRIDRMNPKTLKLRTVVKSGVYGVMGAGTG
                     AIQVGRELWVSSFRADRIARFPENHHGM"
     gene            complement(2052..2582)
                     /gene="nusG"
                     /locus_tag="VFN42_11850"
     CDS             complement(2052..2582)
                     /gene="nusG"
                     /locus_tag="VFN42_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006557411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HET8997352.1"
                     /translation="MAKRWYVVHVYSGFEKKIAQQIKEQAAQKGLADQVDEVLVPSEN
                     VVEVRRGQKVNAEHKFFPGYVLVKMDLTDDAWHLVKNTPKVTGFLGSRLRPTPISDAE
                     AERIMKQTQEGVEHPRPSVLFEVGEQVRVADGPFTSFNGTVEEVDEEKGRVKVSVSIF
                     GRSTPVELEYSQVEKV"
     gene            complement(2598..2792)
                     /gene="secE"
                     /locus_tag="VFN42_11855"
     CDS             complement(2598..2792)
                     /gene="secE"
                     /locus_tag="VFN42_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006557412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET8997353.1"
                     /translation="MAVEPAKFVREVRAEMARVTWPSRKETLVTTGLVFLMVAITAIF
                     FFVVDQAIAVGIRALFGVVG"
     gene            complement(2848..2924)
                     /locus_tag="VFN42_11860"
     tRNA            complement(2848..2924)
                     /locus_tag="VFN42_11860"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2888..2890),aa:Trp,seq:cca)
     gene            complement(3048..3121)
                     /locus_tag="VFN42_11865"
     tRNA            complement(3048..3121)
                     /locus_tag="VFN42_11865"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3087..3089),aa:Gly,seq:tcc)
     gene            complement(3175..3477)
                     /locus_tag="VFN42_11870"
     CDS             complement(3175..3477)
                     /locus_tag="VFN42_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997354.1"
                     /translation="MPGQKSLLSQPAGLAFELADLVMAQGWSAFHDLRMVVELDYCAE
                     GDEYEEVLAFYPPDSAFRRWMIWRGVRGIVVQPMMGRAICYPTVADALEGLIPAGA"
     gene            3854..4024
                     /locus_tag="VFN42_11875"
     CDS             3854..4024
                     /locus_tag="VFN42_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997355.1"
                     /translation="MRACLALCGLESVASEQSLACGDGDKLRTAAGFVYGAMLSGALW
                     SAIVLVAWSLIA"
     gene            complement(4039..4518)
                     /locus_tag="VFN42_11880"
     CDS             complement(4039..4518)
                     /locus_tag="VFN42_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HET8997356.1"
                     /translation="MKSNHAETPDLDAIDRRILAELQADGRITNVELARRAGVSAPPC
                     LRRVRRLEEAGVIRGYHADPDPQKLGWEITFFAIVGLDSQRESVLSAFEQLVADWPEV
                     RECHMIRGGGDFLLKLVARDTAHENQLTQRLTGSATVARVQTLQTIRTSRSLAGVPL"
     gene            complement(4601..5371)
                     /locus_tag="VFN42_11885"
     CDS             complement(4601..5371)
                     /locus_tag="VFN42_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010667935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET8997357.1"
                     /translation="MKIATWNVNSIRQREQHVLRWLQGCQPDLLVLQEIKCEAAAFPA
                     LTFQAAGYQAEIVGQKAYNGVAVLSRIPMTVTHRALPGLPEDDAQARYLEVQAGGITV
                     AGIYLPNGNSRGEDGFAYKLAWMDRLAERAQALLEADTPTVITGDFNVCPADDDFAPG
                     ALPATDALVRPESRARFRRLLWLGLTDAVRAVHPHGPVYTFWDYQAGAWQRDLGLRID
                     HALLSPTLAERLVAAAPDREERAQPQPSDHVPVVIELH"
     gene            5454..>5851
                     /locus_tag="VFN42_11890"
     CDS             5454..>5851
                     /locus_tag="VFN42_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET8997358.1"
                     /translation="MAIDFYTWNTPNGRKISVALEEMGLAYRVHPVNISKDEQFNPDF
                     LKISPNNRIPAIVDPEGPGGQPISIFESGAILIYLGQKTGKFYPTDIRKQVPVLEWLM
                     WQMGGFGPMPGQVHHFLQVENEQDRRYGLER"
BASE COUNT          954 a         1974 c         1928 g          995 t
ORIGIN      
        1 ataccacggc cgcgccgcgc acctggtgca ggccatgatg atcgaggccg ttaccggcca
       61 ggactaccgc gacgtcatcc gccagcgcgt gatcgagccg ctcggcctgg gcggcgacat
      121 cttcgttggg gtgccgccgc agatgcagca tcgctgcgcc gacacctatg cgccggagcc
      181 gcgcgacaat tcagccgagt tccgcgccgc gggcctgccg catggcggcg gcttcgccac
      241 ggcgcgcggc atggcggcgt tctatcagat gctgtgtgcc ggcggccggc tggcaagcgt
      301 gcgcctgttc tcgccgcgcc tgctcgccta tgtcgcgcgc aaccacaccg gggagcgcgg
      361 cgacgtgcag atggacggca tcccgatgca tcgcggcctt ggtccgcatg tacgcggcga
      421 gagcgaccgc atccgcggcc tgggcactat cggcgcgccg cagacgttcg ggcacggcgg
      481 cgtcggctcc tcgtactcgt gggccgatcc gaccagcggc gtgtcgttca cctacctgag
      541 caacttcgtc gtgccggatc cctggcactc ggcacggctg gaccgcgtca gcaacctcgt
      601 gcacgcggcg atcgactgac cggccgcatt ccgggcggtg cggcgactgt tcgcctttgc
      661 gaaggcgatc agggagaaac gcacgccccc tcccgccatc cttggtccac gtcgaaggcg
      721 gcaagggggg agggggcttg cacggtcgct tacatcccgt ggtgattctc cgggaagcgg
      781 gcaatgcggt cggcgcggaa cgagctgacc cagagctcgc gccccacctg gatcgcgccg
      841 gtcccggcgc ccatcacgcc gtacaccccg ctcttcacca ccgtccgcag cttcagcgtc
      901 ttcgggttca tccggtcgat gcggaagggc acgttgcaat tcaccgcgct cgactcgaag
      961 catgcgatca cctgcttcac cgacgcatcc tgcccgccga cgaagatgct gcgcccatcg
     1021 ggcgaccagc gcaggttgtc ggtcaggatg ccggttggca cgctggcctt ccgtaccggc
     1081 atggtgccgc ggctgatgcg cgtcaccgcg tgaccggacc acagtgcgac gtacacttct
     1141 ttcccattcg gcgatgcgat gacgccgttc ggtccggaca tgccggcgct gccgggcacc
     1201 tcggaccagc ctttgccggc ctgccattca tccagcgcgc cgaccggctg gccattcgcc
     1261 agcttgtcca tccggtgcgg atcggttggg tcccacaggc tggtgacgat gatgccgcca
     1321 tccggcaatg aggcgaccgc gtcgggccag aaatgctgtg gcgcgaccac gcagccgacc
     1381 caggtgaaat gcggccgagc cttggtcaga tcgacggcga agacctccac cgactcgcgc
     1441 ccgccgtgat tgacggcata cagggtgcgg tgcgggccct tgccctgcgt caggtccagg
     1501 ccgtgcggac caaagatatt catgtcgagc ggcccggggc aatccttgta gcgcatcttg
     1561 tcgggccgga tggcgatctc ctgcggctct accgggctgg ccttcccggt gcgggtgtcg
     1621 aacaggtaca aggtgccctg cttgcctgcc gccgcgagat cgctaccgat cacccaatgc
     1681 gtgccgggca ccggggcgaa gtcctccacg ttggtgactc cgcaggtgaa cttcacgccg
     1741 gcggccggcg cgcagtcggc ggcgcgcgcc gataccgtgc cgccgacggc gagcagggcc
     1801 gtcagtgccg ttgcgcagaa tatccgcaag tgccgcatcg ctccctcccg aggattgctt
     1861 gttgtgttca acgccgcgcc gcggctgctg ccgcgccggc attgcttgga acgcgcaatg
     1921 ctttgagccg gcgttaagcc gagtcaaagc atacgcgagg ttctactcga gagttgacgg
     1981 cgcggcctgg atggcggcgt tcgttcggcg gatctcgtgc atccgcccta cgatttaggc
     2041 ggcgaaggcg tctaaacctt ctccacctgg ctatattcca gttccaccgg ggtggaacgg
     2101 ccgaagatcg agaccgacac tttcacgcgg cccttctctt cgtccacctc ctccaccgtg
     2161 ccgttgaagc tggtgaaggg gccgtcggcg acgcgcacct gctcgccgac ctcgaacagc
     2221 acggaggggc gcgggtgctc caccccctcc tgcgtctgct tcatgatgcg ttcggcttcg
     2281 gcgtcgctga tcggcgtcgg ccgcaagcgg ctgccgagga agccggtgac cttgggggtg
     2341 tttttcacca ggtgccaggc gtcgtcggtc aggtccatct tcaccagcac gtagccgggg
     2401 aagaatttgt gttcggcgtt gaccttctgg ccgcgccgca cctccacgac gttctcggac
     2461 ggcaccagca cctcgtccac ctggtcggcg aggcccttct gcgcggcctg ttccttgatc
     2521 tgctgggcga tcttcttctc gaagccggag tagacgtgca ccacgtacca gcgcttggcc
     2581 ataaccctga atgctcctca gccaaccacg ccgaacaggg cgcggatgcc gactgcgatc
     2641 gcctgatcga ccacgaagaa gaagatagcg gtgattgcca ccatcaggaa caccagcccg
     2701 gtggtcacca gcgtctcctt gcgggacggc caggttaccc tggccatctc ggcccgcacc
     2761 tcgcgcacga attttgccgg ttcaaccgcc acgctacgaa aactcctgaa acatcatgcg
     2821 gaaccccgcc gcaacggccg cgcctgctgg caggggtgga gggtctcgaa cccccagcct
     2881 ccggttttgg agaccggcgc tctagccaat tgagctacac ccctacacgc gagcagccgg
     2941 gaccgcgcgc cggtcaccgc ttgcgcagag gcggtaaaaa ggccgtggcg gggtccgctg
     3001 tcaagggcgc gggcgacgga ttcttgcgct ggggcaggga agcgaggtgg agcgggtgac
     3061 gggaatcgaa cccgcatagc cagcttggaa ggctggagct ctaccattga gctacacccg
     3121 cgcaggcaac gctttaccgg ttggcgcggg tggccgcaat cgggttgcgg cgggtcaggc
     3181 gccggcgggg atcagccctt cgagtgcgtc ggccaccgtc gggtagcaga ttgcccgtcc
     3241 catcatcggt tggaccacga tgccgcgcac gccgcgccag atcatccaac ggcggaacgc
     3301 gctgtcgggg ggatagaagg cgagcacctc ttcgtactcg tcgccttcgg cgcagtagtc
     3361 cagctccacc accatgcgca ggtcgtggaa cgccgaccag ccttgcgcca tcaccagatc
     3421 agcaagttcg aaggcgagtc ctgctggttg cgacagcagg gatttctggc cgggcattcg
     3481 taacctcgct cttgtcctgg gggccgtctc ctcggggccc gctgatcacg ggcgcgtgat
     3541 ccatagcttc acgggcatgt gatagcaagc attcttattt ggattcaagt tgttgcgcga
     3601 tgccttcgca agttcatttc gaatcataaa atcacaacga accgctaagc gcctgacaaa
     3661 gctgctggcc cccgggcaaa cacttgccgg aaggcaacga acaccattcc gcatcggcaa
     3721 accgcaacga ttcagcggcg ggtgactgcg ccgccgaatc gcgtctgccg gcccacaggg
     3781 ttgtgattgt gatttgccgg ctgctccgga ctctgttact tgccaatcca gacaagcgac
     3841 agaggaagcg gcaatgcggg cgtgtctggc actgtgcggt ctcgaatccg tcgcaagcga
     3901 acagtcgctg gcgtgcggtg atggcgacaa gctccgcacc gccgccggct tcgtctatgg
     3961 cgccatgctg tccggcgcgc tgtggagcgc gattgtcctg gttgcctggt cgctgatcgc
     4021 ctgaattcgc cgcagccgct acagcggcac cccggccagg ctgcggctgg tgcggatcgt
     4081 ctgcagggtc tgcacccgtg ccaccgtggc cgaaccggtc agccgctggg tgagctgatt
     4141 ctcatgcgcg gtgtcccgcg ccaccagctt caacaggaaa tcgccgccgc cacggatcat
     4201 gtggcattcc cgcacctccg gccagtcggc gaccagttgc tcgaaggcgg acagcacgct
     4261 ctcccgctgg ctgtccaggc cgacgatggc gaagaaggtg atctcccagc ccagtttctg
     4321 cgggtccgga tcggcgtgat agccgcggat caccccggcc tcctccagcc ggcgcacccg
     4381 gcgcaggcag ggcggcgctg acacgccggc tcggcgggcc agctcgacat tggtgatccg
     4441 tccgtccgcc tggagctcgg ccaggatgcg gcggtcgatg gcgtccaggt ccggggtctc
     4501 ggcgtgattg gacttcatgc gctcttgctg gcggaaagcg ggcggggcgt gggcggggcg
     4561 cgcaataata ttacacggcg ccgcacagtc cagcggcgat tcaatgcagc tcgatcacca
     4621 ccggcacatg atcggacggc tgcggctgtg cccgctcctc ccggtccggc gcggcggcca
     4681 ccaggcgttc ggccagggtg ggcgacagca gggcatggtc gatgcgcagc cccaggtcgc
     4741 gttgccaggc gccggcctga taatcccaga aggtatagac cggcccgtgc ggatgcaccg
     4801 cccgcaccgc atccgtcaac ccgagccaga gcaggcggcg gaagcgggct ctgctttccg
     4861 ggcggaccag cgcgtcggtc gcaggcagcg cgccgggcgc gaaatcgtca tccgccggac
     4921 acacgttgaa gtcgccggtg atcacggtgg gcgtgtccgc ttccagcaat gcctgggcac
     4981 gctccgccag ccggtccatc caggcaagct tgtaggcgaa gccgtcctcg ccgcgggagt
     5041 tgccgttggg caggtagatg ccggccaccg tgatgccgcc cgcctggact tccaggtatc
     5101 tcgcctgggc gtcgtcctcc ggcaagcccg gcagtgcccg atgggtcacc gtcatgggaa
     5161 tgcgggacag caccgccaca ccgttatacg ccttctgccc gacgatttcc gcctggtagc
     5221 cggccgcctg gaaggtcagc gcgggaaacg cggcggcctc gcacttgatc tcctgcagca
     5281 ccagcaggtc gggctggcag ccctgcagcc agcgcagcac gtgctgctcc cgctggcgga
     5341 tcgagttgac gttccaggtg gcgatcttca tgcgggcgaa ggtgcttgag tcgcggcttg
     5401 tcggcaagcc gggtgccgcg cagagtggac gcaacaaacc ggaggaccga gggatggcga
     5461 tcgacttcta cacctggaac accccgaacg ggcgcaagat cagcgtcgca ctggaggaga
     5521 tggggctggc gtaccgcgtc cacccggtga acatcagcaa ggatgagcag ttcaacccgg
     5581 acttcctgaa gatcagcccc aacaaccgga ttcccgccat cgtcgatccc gaggggccgg
     5641 gcgggcagcc gatcagcatc ttcgaatccg gcgccatcct catctatctc ggtcagaaga
     5701 ccggcaagtt ctacccgacg gacatccgca agcaggtgcc cgtgctggag tggctgatgt
     5761 ggcagatggg cgggttcggc cccatgcccg gccaggtgca ccacttcctg caggtcgaga
     5821 acgagcagga tcgccgctac gggctggagc g
//
LOCUS       DASSZH010000545         2720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1291020, whole genome shotgun sequence.
ACCESSION   DASSZH010000545 DASSZH010000000
VERSION     DASSZH010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291020"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VFN42_11895"
     CDS             complement(<1..334)
                     /locus_tag="VFN42_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997359.1"
                     /translation="MKLRIGLYVVASWLIAAHFLRMDALALAALCLAAPLLFLLHRWW
                     TLPLLQGLAYAAGAVWIVTAWQIVAMRRAFGLPWERSALILLAVAAISLLAGWLVRGV
                     AAARGGRLV"
     gene            complement(371..1036)
                     /gene="ytfE"
                     /locus_tag="VFN42_11900"
     CDS             complement(371..1036)
                     /gene="ytfE"
                     /locus_tag="VFN42_11900"
                     /inference="COORDINATES: protein motif:HMM:NF008221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HET8997360.1"
                     /translation="MSATTAGFADRTLAEIAATLPGATGVFRRHKLDFCCGGRVMLSQ
                     AAADRGLALPELESELDAVAALGLAADRPESTDALITLIETRYHAVHRRELPELVRLA
                     RRVEAVHKHHPAAPTGIATLLEQMEGELEDHMQKEEQVLFPLMRRGGHPMIGQPIAMM
                     LAEHDGHGAHLRRLESLTNDFTAPAEACPTWRALYVGAKKLADDLVEHIHTENNLLFP
                     RFG"
     gene            1451..2224
                     /locus_tag="VFN42_11905"
     CDS             1451..2224
                     /locus_tag="VFN42_11905"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET8997361.1"
                     /translation="MGQLDGKVGIITGAASGIGAACARVLAREGARLLLTDLDDAAGQ
                     RLAEETGSLYQHLDVTYEAAWPAVVASAEQRLGGLHILVANAGIGIMGPAIDMALADW
                     RKQMAVNVDGVFLTVKYGIPAMRRSGGGSVVMMSSVAGLRGSAGLACYSATKGAVRLF
                     AKSMALECAQARDGIRVNSVHPGIIDTPIWGKLPPGANAPIDPRRIAEAAVPLGVPGQ
                     AEDIANGVLFLASDASRHVTGSELVIDGGLTAGRIGRLG"
     gene            complement(2221..2511)
                     /locus_tag="VFN42_11910"
     CDS             complement(2221..2511)
                     /locus_tag="VFN42_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997362.1"
                     /translation="MPALLLRSAAALAVGAMLGGCVYAPPPPVYGYNPYDPGSRAVAG
                     GLVGAGAGAALGGITGGGEGAAIGALAGGALGAAAGAGSTPPPYYAPDYGPD"
     gene            2631..2720
                     /locus_tag="VFN42_11915"
     tRNA            2631..2720
                     /locus_tag="VFN42_11915"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2665..2667,aa:Ser,seq:gga)
BASE COUNT          424 a          941 c          963 g          392 t
ORIGIN      
        1 ggaccaggcg tccgccgcga gcggcggcaa ctccgcgcac cagccacccg gccagcaggc
       61 tgatcgcggc cacggccagc aggatgagcg cactgcgttc ccagggcagg ccgaaggcgc
      121 gccgcattgc gacgatctgc cacgccgtca cgatccagac cgcgccggcg gcataggcca
      181 gcccctgcag cagcggcagg gtccaccacc ggtgcagcag gaacagcagg ggcgcggcca
      241 ggcacaaggc ggccagcgcc agggcgtcca tgcgaaggaa atgcgccgcg atcagccagc
      301 tcgcgaccac gtacaacccg atgcgaagct tcattgccct acccttcctg gcggcggccg
      361 gcggatctgc ctagccgaag cgggggaaca acaggttgtt ctcggtgtgg atatgctcca
      421 ccaggtcgtc cgccagcttc ttcgcgccca cgtacagcgc gcgccaggtc ggacaggcct
      481 cggccggtgc ggtgaagtcg ttggtcaggc tttccagccg gcgcagatgc gcgccatgcc
      541 catcatgttc ggccagcatc atggcgatcg gctggccgat catcggatgc ccgccgcgcc
      601 gcatcagcgg gaacaggacc tgctcctcct tctgcatgtg gtcttccagc tcgccttcca
      661 tctgctccag cagggtggca ataccggtcg gcgccgcggg gtggtgcttg tgcacggcct
      721 cgacccggcg cgcgaggcgc accagctcgg gcagctcacg ccggtgcacg gcgtggtagc
      781 gggtttcgat cagcgtgatc agcgcatcgg tgctttccgg ccgatcggcc gccagaccca
      841 gcgcggcaac cgcgtccagt tcggattcca gctccggcaa ggccaagccc cggtccgccg
      901 ccgcctgcga cagcatcacc cgtccaccgc agcagaaatc cagcttgtgg cggcggaaca
      961 cgccggtcgc acccggcagc gtggcggcga tctcggccag ggtgcgatcg gcgaacccgg
     1021 cggtggtggc gctcatgcgg agacactcct tgcttagtgc cggcgggcct tggcgcccgc
     1081 ggttttgaat tggtattcca gataccaatt cttatgccgg aaaaatttgg tatcgtcaat
     1141 accgattcaa gcgatcgcag acgcccgctc gcctccgccg cgttcgccct gcgttgatgc
     1201 tgctggcgtg cgagccagac ggcagccggg gtcgcgggga gtcgctggcg cagacgctag
     1261 gccggcggtc gcgcaatcat ctgcaaggat cgtgcagggc atcaggccca gggccgatgc
     1321 cggccggctc gctgcccgcg ccatcctgac cggaggggcc gcgccgccag tagatcggcg
     1381 gcccgagtcg atgcagcatg cacccgcatg atgcggcgcg cacgccaccc ggaaaccagg
     1441 aggaatcgtt atgggccaac ttgacggcaa agtggggatc attaccggcg cggcatccgg
     1501 catcggcgcc gcctgtgccc gcgtgctggc gcgggagggc gcgaggctgc tgctcactga
     1561 tctggacgat gccgccggcc agcgcctggc ggaggaaacc ggcagcctgt accagcacct
     1621 ggacgtcacc tacgaagccg cctggcccgc ggtggtggcc agcgcggaac agcgcctggg
     1681 cgggctgcat atcctggtgg ccaatgccgg aatcggcatc atggggccgg ccatcgacat
     1741 ggcgctggcc gactggcgca agcagatggc ggtgaacgtc gatggcgtgt tcctgacggt
     1801 gaaatacggc attccggcga tgcgccgctc cggcggcggg tcggtggtga tgatgtcgtc
     1861 ggtcgccggc ctgcgcggct ccgccgggct ggcctgctac agcgccacca agggcgcggt
     1921 gcggctgttc gccaagtcga tggcgctgga atgtgcccaa gcgcgggacg gcatccgggt
     1981 gaactcggtg catcccggca tcatcgacac gccgatctgg ggcaagctgc cacccggcgc
     2041 caatgctccg atcgatccgc gccgcatcgc cgaagccgcg gtgccgctgg gcgtccccgg
     2101 ccaggcggag gacatcgcca atggcgtgct gttcctggcg tcggacgcct cgcgccacgt
     2161 caccggatcg gagctggtga tcgatggcgg gctgacggcg gggcggatcg gccggctggg
     2221 ctaatcggga ccgtaatccg gagcgtaata gggcggcggc gtgctgccgg cgcccgcggc
     2281 ggcacccagc gcgccaccgg cgagcgcgcc gatcgccgcc ccctcgccgc ctccggtgat
     2341 gccgccgagc gccgcccccg cgccggcgcc gacgaggccg ccggccaccg cgcggctgcc
     2401 gggatcgtag gggttgtagc catagaccgg gggcgggggc gcatagacgc aaccgcccag
     2461 cattgctcca acagcgagtg ccgcggcaga ccgcagcaac agggccggca tgatgggcct
     2521 ccgtgcaaat tgccggaggt gcaacggcac ggccggcctc acagttgccc gcgcgccaga
     2581 cggggttttc ccggcgccca ggcgctgcta tgtgtccggc gccgtgcgca ggagagatgg
     2641 ccgagcggct taaggcgcac gcttggaaag cgtgtgtacg tgcaagcgta ccgtgggttc
     2701 gaatcccact ctctccgcca
//
LOCUS       DASSZH010000546         5169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_132328, whole genome shotgun sequence.
ACCESSION   DASSZH010000546 DASSZH010000000
VERSION     DASSZH010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132328"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="VFN42_11920"
     CDS             <1..1254
                     /locus_tag="VFN42_11920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET8997363.1"
                     /translation="RADVVIIGGGIIGASTALFLAEKGHSVVLCEKGRIGGEQSSRNW
                     GWCRTMGRDNREIPLALESLRLWRGMNQRTGQETGFRQAGIVYYCETDKEAAEQEAWL
                     DQAREYQLEARMLRGSDVAAVAPGATIPFVAALHTPTDGRAEPSHAAPAIAEAARAHG
                     ATILTGCAVRSIDLQAGRIAGVVTEQGRIACDAVVLAGGAWSRLFCGNAGIDLPQLKV
                     LASVFRTTPVDGPEVTGGTSVFAFRKRLDGGYTVARRNRNVADITPDSFRLLLDYLPT
                     LRQNYGEIRMRVSRRFFEEWGIKRRWSPDQPTPFEAVRVLDPAPRQATLAEGRAALAH
                     AFPAFAKAGVAESWGGMIDVTPDAVPVISAVDRVPGFFIATGFSGHGFGIGPGAGRLM
                     AELVAGDTPVVDPAPFRLDRFTRTR"
     gene            1267..2235
                     /locus_tag="VFN42_11925"
     CDS             1267..2235
                     /locus_tag="VFN42_11925"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET8997364.1"
                     /translation="MKFAANLSPSRRQQLMFGYLCFVWGTTWLGMKVGIATVPPALFA
                     GTRWTIAGLVLLAIRYARGESIMPPPRLALRLLLVAVLMVSMTSAIQMYGLRYITAGL
                     AAVITSALSPVALLGFSVALGQDRFSPRQLGAIALGVAGVVVLFGPSALAGKLDVWEM
                     LGAAGVIIGCLSYSLGSVLARPVMRSLAPVQVSGLTNLIGGFALLAGALAFDPGSRAA
                     VTHLWAWPAALAWLYLLVPGSLVATTIYFLLVRDWGPTRTGTYAFVSPVIAVVLGCAL
                     FGEQVDWGDAAGMLLMLLGAGLALRPAALAKAMPRVNRRRISMRAG"
     gene            complement(2251..2679)
                     /locus_tag="VFN42_11930"
     CDS             complement(2251..2679)
                     /locus_tag="VFN42_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997365.1"
                     /translation="MRKTVIAAVTALAVTGLGAGTLVANAQPAPPPGPPAGPGFGGPP
                     GPHPHWHAWMHHRGEEHRPFAPGTFALVHRQADRHLSPSDVQTIAQAFLLWNGNHSWK
                     VVDVAPGPDGKIGFAYATQQGGVIARFTMDPHNGHVARVD"
     gene            2812..3531
                     /locus_tag="VFN42_11935"
     CDS             2812..3531
                     /locus_tag="VFN42_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007282658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8997366.1"
                     /translation="MEQMPHIMVVDDDREIRDLLGRFLEKHRLRVTTVRDAREARRAW
                     TNGHYQLIVLDLMLPGESGLDLARWLRSVSDVPIVMLTAMGEETDRIIGLELGADDYV
                     PKPFNPRELLARIRAVLRRAGDAPAARHEAGSRPLRFADWTLEPARRRLLNPEGAEVA
                     LTGGEYDLLLALAERPNRVLTRDMLLDLLRGRQAGPFDRAIDVAISRLRRKLEDDGRN
                     AQLIKTVRGGGYVLAASVERL"
     gene            3528..4901
                     /locus_tag="VFN42_11940"
     CDS             3528..4901
                     /locus_tag="VFN42_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8997367.1"
                     /translation="MTPHLWPRSLATRTAVVLLAGLIVVQIAGLTIHAFDRMDLQRLE
                     AAREVAIRAMGLYRAVVMTSPEERQKVLGEVHRAPGVSAVLSPTPPLGGMPELPYRLQ
                     RILRLSITLVPLPPNLRPHDFRMLGGVGFPTTVVGLRLPEGDWLNVTLPAPSVSPWHS
                     PAFLTAFLLMTVVAAILTLWAVRRLTAPVRTLAAAAEALGRDVNAPPLPEDGPTEVAT
                     AAAAFNTMAARIRRFVQDRTELLTAIGHDLRTPITRLKLRAEFIEDEEQQRKMLADLE
                     ELEAMVSATLAFGRDAASAEPVTPLDLAELLRTVLDEAGDAQPELADQLAYEGPSHFT
                     LRARPLAMKRALSNLVTNALNYGGDARVRLTPPQSLGAAGRVVTIEVEDNGPGIPPSD
                     LERVFEPFHRGEPSRSRETGGVGLGLPIARNIFRAHGGDVVLANKPAVSPGQRVGGVK
                     ATVTLPV"
BASE COUNT          733 a         1722 c         1848 g          866 t
ORIGIN      
        1 cgtgccgatg tggtcatcat cggtggtggc atcatcgggg catcgaccgc gctttttctc
       61 gccgagaagg gccattcggt ggtgctgtgc gagaaggggc gcattggcgg cgagcaatcc
      121 agccggaact ggggctggtg ccgcacgatg gggcgggaca accgggaaat cccgcttgcc
      181 ctggaaagcc tgcggctctg gcgcggcatg aaccagcgca ccgggcagga gaccgggttc
      241 cgccaggccg gcatcgtgta ctattgcgag accgacaagg aagccgccga gcaggaagcc
      301 tggctggacc aggcccgcga gtatcagctg gaggcccgga tgctgcgcgg cagcgacgtt
      361 gccgcggtcg cccccggcgc taccatcccc tttgtcgccg cgctgcacac gccgactgac
      421 ggccgggcgg aaccatccca cgccgcgccg gcgattgccg aagcggcacg ggcgcacggg
      481 gccaccatcc tgacgggttg cgcggtgcgc agcatcgacc tgcaagcggg ccggatcgcc
      541 ggcgtggtta ccgagcaggg gcgcatcgcc tgcgacgctg tggtgctggc gggaggggcg
      601 tggtcgcgac tgttctgcgg caatgccggg atcgacctgc cgcaattgaa ggtgctcgcc
      661 tcggtgttcc gcaccacacc agtcgacggg ccggaggtca ccggcggcac cagcgtcttt
      721 gccttccgca agcgtttgga cggcgggtac acggtggcgc ggcgcaaccg caacgtggcc
      781 gacatcacgc cggattcctt tcgcctgctg ctggattacc tgcccacgct gcgacagaac
      841 tacggcgaga tccgcatgcg ggtcagccgc cgtttcttcg aggaatgggg catcaagcgc
      901 cgctggtcgc cggatcagcc gacgccgttc gaggcggtcc gcgtcctgga tccggccccg
      961 cgccaggcca cccttgcgga aggccgcgcc gcgctggccc acgccttccc ggcattcgcc
     1021 aaggctgggg ttgcggaaag ctggggcggc atgatcgacg tcacgcccga tgcggtgccg
     1081 gtcatctctg cggtggatcg cgtgcccggc ttcttcatcg ccacgggctt ttccggccac
     1141 ggcttcggca tcggcccagg tgccggccgg ctgatggcgg aactcgtcgc gggggacacg
     1201 ccggtggtcg atcccgcgcc cttccgtctc gatcgcttca cccgcacccg gtagccctcg
     1261 cacgctatga agttcgccgc caacctgtcg ccgtcccggc gccagcagct gatgttcggt
     1321 tatttgtgtt tcgtctgggg caccacctgg ctgggcatga aggtgggcat cgccaccgta
     1381 ccgcccgcat tgttcgccgg cacgcgctgg accattgccg ggctggtgtt gctggcaatc
     1441 cgctacgccc gcggggagag catcatgccg ccgccgcgcc tggcgctccg gctgcttctg
     1501 gttgccgtcc tgatggtgtc gatgacctcc gccatccaga tgtacgggct gcgctacatc
     1561 accgccggcc tcgccgcggt gatcacctcg gccttgagcc cggtcgcgct gctcgggttt
     1621 tccgtggcgc ttggccagga ccggttcagc ccacgccagc tcggcgccat cgcgttgggc
     1681 gtggctggcg tcgtggtgct gttcggcccc tcggctctgg ccggcaagct ggacgtctgg
     1741 gaaatgcttg gcgcggccgg cgtgatcatc gggtgcctga gctactccct cggctctgtg
     1801 ctggctcggc cggtgatgcg cagcctggcg ccggtgcagg tgtcggggct taccaatctg
     1861 atcggcggtt ttgcgctgct ggcgggggcc ctcgcgtttg atcccggttc ccgcgcggcc
     1921 gtcacgcatc tctgggcctg gccggcggcg ctggcctggc tgtacctgct ggtgcccggg
     1981 tcgctggtcg ccaccacgat ctacttcctg ctggtacgcg actggggccc gacccgcacc
     2041 ggcacctatg ccttcgtttc gccggtaatt gccgtcgtgc tgggctgcgc gctgttcggc
     2101 gagcaggtgg actggggcga tgcggccggc atgctgctga tgctgttggg ggcgggcctg
     2161 gcattgcgcc cagcggcgct ggcgaaggcc atgccacggg tcaaccggcg gcgcatcagc
     2221 atgcgcgccg gctgacccag gcgtagaagt tcagtccacc cgcgcgacgt ggccgttatg
     2281 cgggtccatg gtgaagcggg caatcacgcc accctgctgg gtggcatagg cgaagccaat
     2341 cttgccgtcc gggcccggcg cgacgtccac caccttccaa ctgtgattgc cgttccacag
     2401 caggaacgcc tgggcgatgg tctgcacgtc gcttggactc aggtgccggt ctgcctgacg
     2461 atgcaccagc gcgaaggtgc ccggcgcgaa gggccggtgc tcctcccccc ggtgatgcat
     2521 ccatgcatgc cagtgcggat gcggccccgg tggtccgccg aagcccggac ccgcgggcgg
     2581 gccgggcggc ggagcgggct gcgcgtttgc aaccagcgta ccggcaccca aaccggtgac
     2641 ggccaatgca gtgacggcgg cgatgacggt ctttcgcatt ctgtcctccc gaattgcgag
     2701 caccggcaat gtggaggacg cgcattgcag ccgtgccgcg gtttggcgac gaagtgcaac
     2761 ggagtgcaac gcaccctgcc gccggtcgcg gtttgtgcct attattccgg catggagcag
     2821 atgccccaca tcatggtggt ggacgacgac cgggaaatcc gcgacctgct tggccgcttc
     2881 ctggaaaagc accgcctgcg ggtcaccacc gtacgcgacg ctcgcgaagc gcggcgtgcc
     2941 tggaccaacg gccactacca gctgatcgtg ctggacctga tgctgcccgg ggaatccggc
     3001 ctggatctcg cccgctggct gcgcagcgtg tcggatgtgc cgatcgtcat gctcaccgcg
     3061 atgggcgagg agaccgaccg catcatcggc ctggaactgg gcgccgacga ctacgtgccc
     3121 aagccgttca atcccaggga gctgctggcc cgcatccgcg cggtgcttcg ccgtgccggt
     3181 gacgcgccgg ccgcccggca tgaggccggc agccggccgc tgcgcttcgc cgactggacg
     3241 ctggagccgg cccgccgccg cctgctcaat ccggaaggcg ccgaggtggc actgaccggc
     3301 ggcgaatacg acctgctgct ggcactggcg gagcggccga accgggtgct gacccgggac
     3361 atgctgctgg atctgctgcg cgggcggcag gcagggccgt tcgaccgggc gatcgacgtc
     3421 gccatctctc gcctccgccg caagctggag gatgacggcc gcaatgcaca gttgatcaag
     3481 accgtgcgcg gcggcggcta cgtgctggcg gcctcggtgg agcggttgtg acgccgcacc
     3541 tgtggccacg cagcctggcg acgcggaccg cggtggtgct gctggccggg ctgatcgtgg
     3601 tgcagatcgc cggcctgacc atccacgcct tcgaccgcat ggatctgcag cggctggagg
     3661 ccgcgcgtga agtcgcgatc cgcgccatgg gcctgtaccg cgcggtggtg atgaccagtc
     3721 cggaagaacg gcagaaggtg ctgggtgagg tccaccgtgc gcctggtgtg tctgccgtgc
     3781 tcagcccgac cccgccactg ggtggcatgc cggagctgcc gtaccggctg caacgcattc
     3841 tgcggttgag catcaccctg gtaccgctgc cgccaaatct gcgcccacat gatttccgca
     3901 tgcttggtgg cgtaggtttt cccacgaccg tcgtcggatt gcgtctgcct gagggcgatt
     3961 ggctgaacgt gaccctgccc gcgcccagtg tcagcccgtg gcactcgccg gccttcctga
     4021 ccgcgttcct gttgatgacg gtggtggcgg cgatcctgac gctatgggcg gtgcgccggc
     4081 tgacggcccc tgtccgcacg ctggccgccg ccgccgaagc gcttggccgg gacgtgaatg
     4141 cgccgccgct gccggaggac gggccgaccg aggtcgccac cgccgccgcc gcgttcaaca
     4201 ccatggcggc ccgcatccgc cgcttcgtgc aggaccgcac cgagttgctg accgcgatcg
     4261 gacacgacct gcgcacgccg atcacccgcc tgaagctgcg tgccgagttc atcgaggacg
     4321 aggaacagca gcgcaagatg cttgccgacc tggaggagct ggaggcgatg gtctccgcga
     4381 cgctcgcctt cggccgggat gcggccagcg cggagccggt gacgccgctg gacctggcgg
     4441 agctgctgcg caccgtgctg gacgaggcgg gcgacgcgca gccggaactg gctgatcaac
     4501 tggcctatga ggggccgtcg cacttcaccc tgcgcgctcg gccgctggcg atgaaacggg
     4561 cgctgtcgaa cctggtgacg aacgcgctga attatggtgg cgacgcgcgg gtacggctga
     4621 ccccgccgca gtcgctgggt gccgccgggc gggtggtgac gatcgaggtg gaggataatg
     4681 gtcccggcat tccacccagc gacctggagc gggtgttcga gcccttccac cggggggagc
     4741 cgagccgatc gcgggagacc ggtggggtgg gtctgggcct gccgatcgcg cgcaacatct
     4801 tccgggcgca tggcggggac gtggtgctgg cgaacaagcc cgcggtcagt cccggccagc
     4861 gggtcggcgg ggtgaaggcg acggtcacgc tgccggtgtg agggggctgc gcgccaagca
     4921 aactgtacga ttgaattgca ccgcgggcga ggcgggctta tttggatccg ggattctgtg
     4981 agacgaacga tgaatgcttg gcaggcatcc gcggcccgga accgcttacc cgagatcatc
     5041 gaccaggcgg ccggaggcaa tccgcaactc atccagcggc gtgatgggaa agaggtggtt
     5101 gtggtctccc gcgagtacta cgagcgcacc agagagaatc tgaagagcta ccttctcggg
     5161 aatggatat
//
LOCUS       DASSZH010000547         3315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1257122, whole genome shotgun sequence.
ACCESSION   DASSZH010000547 DASSZH010000000
VERSION     DASSZH010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257122"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VFN42_11945"
     CDS             <1..744
                     /locus_tag="VFN42_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997368.1"
                     /translation="ARSAAVSRGPDGTTLGFLGRAHPLVRRAIMRGMCGPNPLSAARV
                     GRDAAGSALLTYSVELHSGSSLAMRRVIAVLVPASGPVAALMHPKDWLHYADPDRTTA
                     VTPAVLRGATGWLASRQAEAMSCAHAAARHMAEAFARTHRRRMQAATDALQAWLRLRT
                     KEICGAAERCEADLFGAAPSGPAWRTPLPALERLAAFARDAENPPGRRREANSVVALF
                     SAREAEVAEQTALSAPVLRPLGLLMLVAA"
     gene            748..>3315
                     /locus_tag="VFN42_11950"
     CDS             748..>3315
                     /locus_tag="VFN42_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997369.1"
                     /translation="MGARPSQAPHAAEADTDLLLCDCALAGPALPEPFVRFELTRFLQ
                     SRRLWPGTRVAQFWPDIRQHLASLGTAAGPLRVQHQLLVPLASCLGYATPVRAEPVQT
                     REGAEDGGWLMQAANGTRLRGWTVGTETDLDAPIRSGRAYRFSPTRCAQRVLLASGER
                     AGLLSNGDELRLLFCDPARPDSHLAIRLGAWRDQRDPPASFRLLLALAAPQGLAALPE
                     LFEAGRLSQVRITKDLRVQARAAIEGFLQGILDAPANGPALAALDRESLPRVLWQESL
                     VLVYRLLFILKMEAAGDPARAFSFASTQLWRHALSPNQALGALVRRSIDQAQETGRML
                     EDGLRVLFRAFRDGLCCSELRIAALGGALFGPDATPTLDALAWGDRGVALLLDRLLWT
                     SSCGRGRERVHYGALDVEELGRVYEALLELTPGIATAPMLRLRRAKLEVVIPASTKCG
                     QAEAVERIAAGRFYLRAGFGRKATGSYYTPHVFVRYLVRETLLPQVRQRSPDDDPNPA
                     AILALKLVDPAMGSGHFLVEACRFLGDALYAACRLCDELAAAADAAADRAQGEKQGKL
                     RQRAAALRARLAALPDPGGILCAYLPNHSCDADGGFSGPHAVAYCRRLIAVHCLYGVD
                     RNVLAIELAKLSLWLESYAEGLPLTFLDHRLLHGDSIGAPFFADLAALPVGATPLLGV
                     VTFGIQERLDAATGRARREIRTLEASVGRDAADMVLKQTAKQRLDHILQPLRLLARAW
                     SGAAALGRPECDAAWLDLARHVARRDAWPRLLTPIQAAMLQAGALALPWDLTFPEVFA
                     SDTGSGFDAVLGNPPWDVVQYNTREFVAAFDLRVLDAATKQEAARIERRVLADPTVAG
                     "
BASE COUNT          467 a         1142 c         1168 g          538 t
ORIGIN      
        1 gcgcgctcgg ccgcggtgtc gcgcggtccg gatggcacca cgcttggatt cctcggccgc
       61 gcacatccgc tggtgagacg ggcgatcatg cgggggatgt gcggaccaaa cccgctgagc
      121 gccgcgcggg tcgggcgaga cgcggcaggt tcggcgttgc tgacctattc ggtggaactg
      181 cacagcggat ccagcctggc gatgcggcgc gtgattgccg tgctggtgcc ggcgtccgga
      241 ccggttgcag cgctcatgca cccgaaggat tggctgcact acgccgatcc ggatcgcacg
      301 acagcggtca cgccggcggt cctgcgcggc gccaccggct ggcttgcatc gcggcaggcg
      361 gaagcaatga gttgcgcaca tgccgccgct cggcacatgg ccgaggcatt tgcccggacg
      421 catcgccgcc gcatgcaggc agccaccgac gcgttgcagg cgtggctgcg cctgcggacc
      481 aaggagattt gcggcgccgc cgagcggtgc gaagcagacc tgttcggtgc cgcgccatcg
      541 ggcccggctt ggcgaacccc attgccagcg ctggaacggc tggcggcgtt cgcacgcgac
      601 gccgaaaacc cgcccgggcg ccgccgggag gcgaacagcg tggtggcgtt gttctctgcg
      661 cgggaagcgg aggtcgcgga acagaccgcc ttgtcggccc cggtgctgcg gccgctggga
      721 ctgctcatgc tggtagcagc ctgatcgatg ggcgcccgtc cctcccaggc gccgcatgcc
      781 gccgaagccg acaccgacct tctcctctgc gactgcgcgc tggccggtcc cgcgctgccg
      841 gagcccttcg tgcgcttcga actcactcgg tttctgcaat cccgccggct gtggccaggg
      901 acccgcgtcg cccagttctg gcccgatatc cgccagcacc tcgcaagcct gggcaccgca
      961 gccgggccgc tgcgcgtcca gcatcagttg ctcgtgcccc tggcatcctg tctcggctac
     1021 gcgacgccgg ttcgcgcgga acccgtgcaa acacgcgaag gggcggaaga cggcggctgg
     1081 ctgatgcagg cggcaaacgg cacccggctc cgcggctgga ccgtcggtac cgaaaccgat
     1141 ctcgacgcgc caatccgcag cggcagggcc taccggttca gtccgacccg ttgcgcccag
     1201 cgcgtgctgc tcgcgagcgg cgaacgcgcc gggctgctga gcaacggtga cgaactgcgc
     1261 ttgctgttct gcgatccggc acggccggac agccatctcg cgatccggct gggtgcgtgg
     1321 cgcgatcaac gcgatccgcc cgcgtcgttc cgcctgctgc tggcgctggc agcgccgcag
     1381 ggtctggcgg ctctgccaga gctattcgag gccgggcggt tgagccaggt ccgcatcacc
     1441 aaggatctgc gcgtgcaagc ccgtgcggcg atcgagggat ttctgcaagg catactcgat
     1501 gctcccgcga acggtccagc gctcgcggcg ctggaccgcg agtcgctgcc gcgcgtgctg
     1561 tggcaggaaa gcctggtgct ggtctatcgc ctgctgttca tcctgaagat ggaggcggcc
     1621 ggcgatccgg cccgcgcctt cagctttgcc tccacgcagc tatggcggca cgcgctgtcg
     1681 ccgaaccagg cgctgggggc gctggtccgc cgcagcatcg accaggcaca ggaaaccggc
     1741 cgcatgctgg aggatggcct tcgcgtcctc ttccgtgcgt tccgcgacgg gctttgctgc
     1801 agcgagttgc gcattgccgc cctgggcggt gcgctgttcg gaccggatgc aacgccaact
     1861 ttggacgcac tggcctgggg cgatcgcggg gtcgcgctgc tgctcgaccg gctgttgtgg
     1921 acgtcatcct gtggccgcgg ccgggagcgc gtgcactacg gtgccctgga cgtggaagaa
     1981 cttggccgcg tgtatgaggc gttgctggaa ctgacaccgg ggatcgccac cgcacccatg
     2041 ctccggctgc gacgtgcgaa gctggaggtc gtcattccag cctccacgaa atgcggccag
     2101 gccgaagcgg tggaacgcat cgccgccggc cgcttctacc tgcgcgccgg attcggccgg
     2161 aaggcaacgg gatcgtatta cacgccgcat gtgttcgtgc gctacctggt gcgcgaaacg
     2221 ctgctgccgc aggtccgcca gcgcagcccg gacgacgatc cgaacccggc cgccatcctg
     2281 gcgctgaaac tggtcgatcc ggcgatgggc agcgggcatt tcctggtgga agcctgccgg
     2341 ttcctgggtg acgcgctgta tgcggcctgc cggttgtgcg acgaactggc cgccgcggcc
     2401 gacgctgccg cggaccgcgc gcagggtgaa aagcaaggca aactgcggca acgtgcggcg
     2461 gcgttgcgtg cgcggctcgc tgcgctgcca gaccccggcg ggatcctgtg cgcctatctg
     2521 ccgaaccata gctgcgacgc ggacggtgga ttttccggac cgcacgccgt cgcctattgc
     2581 cgccggctga tcgcggtgca ctgcctgtac ggcgtcgacc gcaacgtcct ggcgattgag
     2641 ctggccaagc tgtcgctgtg gctggaatcc tatgcggaag gcttgccgct gacctttctc
     2701 gatcaccgcc tgctgcacgg cgattcgatt ggcgcgccat tctttgccga cctcgctgca
     2761 ctgccggtcg gcgccacgcc gctgcttggc gtggtgacat tcggcatcca ggagcgcctg
     2821 gacgccgcca ccggccgcgc acggcgggaa atccgcacgc tggaagccag cgtcggccga
     2881 gacgccgcgg acatggtgct gaaacagacc gccaagcagc ggctggatca tatcctgcag
     2941 ccattgcgcc tgctggcgcg cgcctggtcg ggcgcggcgg cgttggggcg gccggaatgt
     3001 gatgccgcct ggctggacct ggcacggcat gtcgctcggc gcgatgcgtg gccgcgactt
     3061 ctgaccccaa ttcaggcggc gatgctgcaa gcgggcgcgc tggcgctacc ctgggatctg
     3121 acctttccgg aggtgttcgc cagcgacacc ggctccggct tcgacgccgt gctcggcaat
     3181 ccgccctggg atgtcgtgca gtacaacacg cgcgaattcg tcgccgcctt cgacctgcgc
     3241 gtgctggatg ccgccaccaa acaggaagcc gcacggatcg agcgccgcgt gctggccgat
     3301 ccgaccgtcg ccggc
//
LOCUS       DASSZH010000548         4748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_490141, whole genome shotgun sequence.
ACCESSION   DASSZH010000548 DASSZH010000000
VERSION     DASSZH010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490141"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1288
                     /locus_tag="VFN42_11955"
     CDS             <1..1288
                     /locus_tag="VFN42_11955"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HET8997370.1"
                     /translation="SDPAAMLLARGENPHPVQLTRPPVAPLSAMARLGRRIFFDSSLS
                     AAGRLSCASCHDPANHYGPPPHTGPVMLGGAHLTRQGLRAVPTLTYLERQPGFSIGPD
                     NAENETAPNPQTVALRVHGPRPEKIAGDPAPSAAALVPQGGLFWDGRVDTLQQQALQP
                     LLNPVEMANASAASVAAKLRRAPYVRRFVPLFGPSILHDPRLLLSEAMFAVARYQIED
                     PSFHPYSSKYDAWLEGKARLTPAEMRGYRLFNDAKTANCAGCHLDQPRADGTPPRFTD
                     GQFEALGVPRNPALTANRNPHFHDLGLCGPVRQDLRADTQYCGMFLTPTLRNVATRHA
                     FFHNGVFHSLRQVLDFYRFRDTDPARVYPAGADGRPATFNDLPPRYRGNVDVTDPPFD
                     RKPGYQPAFTARDAADIIAFLRTLTDGYKPEHGGRR"
     gene            1329..1568
                     /locus_tag="VFN42_11960"
     CDS             1329..1568
                     /locus_tag="VFN42_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007396143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997371.1"
                     /translation="MPDTTLRPAQPDEIAEALAFALRYEGRKRVHHADDVMARITAER
                     LVRHLERCGFVLMRAPGAAAPSSSRVMPPLPQGES"
     gene            1762..2997
                     /locus_tag="VFN42_11965"
     CDS             1762..2997
                     /locus_tag="VFN42_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HET8997372.1"
                     /translation="MDASIPPKAAGKGLARSPGALAGIKVIDLTRVLGGPYCTMILSD
                     HGADVIKVEPPQGDEVRDWGPPFHDDDASYFVGVNRNKRAIALDISKPDGQAVLLRLL
                     RDADVLIENFKPGAMEKWGIGYDVLSQRFPGLIHCRISGFGGDGPLGGLPGYDAILQA
                     MTGLMSVNGNSETGPMRLGTAIVDMGTGLYSAVGILMALHERSRSGRGQYLDMTLYDC
                     GMALLHPQAANYFLNDKRPAGTGNPHPNLVPYDKYPTRTCEIFIASGNNGQFRKLCDL
                     IGRPDMAEDPRFANNGARSVNRDALGAELAKAFADEDGHALALKLIRNGVPAGPVLFV
                     DETAAAPHTAHREMVTELDWYKGIGTPIKFSRTPGGTRRPPPKFAQDADAVLKQHGYS
                     DDDIAAMQRNGILHRTRRT"
     gene            3145..>4748
                     /locus_tag="VFN42_11970"
     CDS             3145..>4748
                     /locus_tag="VFN42_11970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8997373.1"
                     /translation="MAFTDIPAYERLRLAGETAITWAGFDSEWYLAAYPDVAPQLADR
                     SPATVLAWYLDHGQQRGDSPNIVFDEAWHRQVYPDVAAAIRAGEVMSAFDSYCRGGFR
                     TRSPHWLFDEGYYRRRYPDITDAALENAELANGYDHFLRHGNLEQRIGHLFFNPGLYC
                     ADLEPDGAERARTQGPFLHYLRRIRARAPEPRTTAYFDPAWYLARYKEVAEAIAAGTW
                     MCALQHYLCNDTPTAFDPLPDFSEPYYLTHNHDLAPAIGRGDLRNGYMHFLLFGAREL
                     RAPSPAIDLHYYARQEPVRADLEQGLAPDAFWHWLRIGRSKGLNAAQPPEETVTEGQA
                     RTLWRRRGQALLPLLARHPLDFTCATAPVVSVVMLLKDEFAQTLLTLAALRASFAGAI
                     ELVLIDAGSTDETRHIGRYVRGAQLLPLEMDVDLVAGRNAALYAVTAEYVLMLQTGVE
                     LAPGALAAALRRMQAEPSVGAVGGRMIKPHGMLAEAGCIVWHDGTTLSYLRDADPLHP
                     EASFRRSVDFCGSSCLLLRTAPLRDI"
BASE COUNT          781 a         1689 c         1509 g          769 t
ORIGIN      
        1 gtcagacccg gctgccatgc tgctggcgcg cggcgagaat ccgcatccgg tgcaactgac
       61 gcggccgccc gtcgcgccgc tgtccgccat ggcacggctg ggccggcgga tcttcttcga
      121 tagcagcctg tccgccgccg gacgcctgtc ctgcgcttcc tgccacgacc cggccaatca
      181 ctacggcccc ccgccccaca ccggccccgt gatgctcggc ggcgcgcatc tgacccggca
      241 gggcttacgg gcggtgccga cgctgaccta tctcgaacgc cagcccggct tcagcatcgg
      301 ccccgacaat gcggagaacg agaccgcgcc caacccgcag accgtggcgc tgcgggtgca
      361 cggcccgcgt ccggagaaga tcgccggcga tccggcgcct tcggccgcgg cactggtgcc
      421 gcagggcggg ctgttctggg atggccgggt ggacacgctg cagcagcagg cgctgcagcc
      481 gctgctgaac ccggtggaaa tggccaatgc cagcgcggcg tccgtggcgg ccaagctgcg
      541 gcgggcgcct tacgtgcggc gcttcgtgcc cttgttcggc ccgtcgatcc tgcacgaccc
      601 gcgcctgctg ctgtccgaag cgatgttcgc ggtcgcccgc taccagatcg aagacccgtc
      661 attccatccc tacagcagca agtacgacgc ctggctggag gggaaggcgc ggctgacacc
      721 ggccgagatg cgtggctacc ggctgttcaa cgatgcgaag acggcgaatt gcgccggctg
      781 ccacctggat cagccgcggg ctgacggcac gccgccacgc ttcaccgacg gccagttcga
      841 ggcattgggc gtgccgcgca acccggcgct gacggccaac cgcaacccgc acttccacga
      901 cctggggctg tgcgggccgg tgcggcagga tctgcgggcg gacacccagt attgcggcat
      961 gttcctgacg ccgaccctgc gcaatgtcgc cacccggcac gcgttcttcc acaacggcgt
     1021 gttccattcg ctgcggcagg tgctggactt ctaccgtttc cgcgacaccg atccggccag
     1081 ggtctatccg gcgggagccg atggaaggcc ggcaacattc aacgacctgc cgccccgcta
     1141 tcgcggcaat gtcgacgtga cggacccgcc gttcgaccgc aagccgggtt accagccggc
     1201 gttcaccgca cgcgacgcag ccgatatcat cgcattcctg cgcacgctga ccgatggcta
     1261 caagccggag cacggcggga gacgctgaag gttcttgtct tgttctcttg gggcggctat
     1321 cctcggccat gcccgacacg acgcttcgcc ccgcgcaacc cgacgaaatc gccgaggccc
     1381 tggcctttgc gcttcgctac gaaggacgca aacgcgtcca ccacgccgac gacgtcatgg
     1441 cgcgcatcac cgccgaacgc ctggtgcggc acctggagcg gtgcggcttc gtgctgatgc
     1501 gcgcaccggg cgccgcagcg ccgtcttcct cccgggtgat gccgccgctt ccgcaaggcg
     1561 aaagctgacg cgccattgac ctgcatcagt gccggccggt gcggcagccg gcatgctggc
     1621 accgcttctc ccaagcaaga tcttccccaa cctgccccgg cccgccaagt gccggggttt
     1681 tctttgtccg gggtttttct ttcgcgcgcg ccgcctggcc gccctctccc tccgatcaca
     1741 cggcggtgct aaagccggtg catggacgca agcattcccc ccaaggccgc gggaaagggc
     1801 cttgcccgct cccccggcgc cctcgccggc atcaaggtca tcgacctgac ccgcgtgctg
     1861 ggcggcccgt attgcacgat gatcctgtcc gaccacggcg ccgacgtgat caaggtcgag
     1921 ccgccccagg gcgacgaggt acgcgactgg ggcccgccct tccatgacga cgacgccagc
     1981 tacttcgtcg gcgtcaatcg caacaagcgc gccatcgcgc tggacatcag caagccggac
     2041 ggccaggcgg tgctgctgcg cctgttgcgc gatgccgacg tgctgatcga gaatttcaag
     2101 cccggcgcga tggaaaaatg gggaatcggc tacgatgtgc tgtcgcagcg tttccccgga
     2161 ctgatccact gccgcatctc cggctttggc ggcgacggcc ccctgggcgg gctgcccggc
     2221 tacgacgcca tcctgcaggc gatgaccggg ctgatgagcg tcaacggcaa cagcgaaacc
     2281 ggtccgatgc gcctcggcac tgcaatcgtc gacatgggca ccggcctgta ttccgcggtc
     2341 ggcatcctga tggcgctgca cgaacgcagc cgctcggggc gcggccagta tctcgacatg
     2401 acgctgtatg attgcggcat ggcgctgctg catccgcagg cggccaatta tttcctgaac
     2461 gacaagcgcc cggccggcac cggcaatccg catccgaacc tggtgccgta cgacaaatac
     2521 ccgacgcgca cgtgcgagat tttcatcgcc agcggcaaca acggccagtt ccgcaagctg
     2581 tgcgacctga tcggccggcc ggacatggcg gaggatccgc ggttcgccaa taacggcgcc
     2641 cgcagtgtca atcgcgatgc actgggcgcc gaactggcca aggcctttgc cgacgaggac
     2701 gggcacgcgc tggcgctgaa gctgatccgc aatggcgtgc cggcaggacc tgtcctgttc
     2761 gtggacgaaa cggccgccgc accgcatacc gcgcaccggg aaatggtaac cgaactggac
     2821 tggtacaagg gcatcggcac accgataaaa ttctcccgca cgccgggtgg cacgcgccgg
     2881 ccgccgccga aattcgcgca ggatgccgat gcagtgctga agcagcacgg ctactcggac
     2941 gacgacatcg cggcgatgca gcgcaacggc attctgcacc gcacacggcg cacctgaagc
     3001 gcacgcatga acggctggta ttgcgatgcg ctaaaaatcc gcgcgcatcg cccggcgcga
     3061 agccggcgaa tcggccaaat gcggcaatat catttcggcc acgcccaggt ataatcgttg
     3121 gtggattcgc ccaggaacag ccgaatggca ttcaccgaca ttcctgccta cgagcgcctg
     3181 cgccttgcag gtgagaccgc aattacctgg gcaggcttcg attccgaatg gtacttggcc
     3241 gcctatccgg atgtcgcccc gcaactggcg gatcgttcgc ccgccaccgt gctcgcctgg
     3301 tatctggatc acggccagca gcgcggggat tcaccgaaca tcgtcttcga cgaagcctgg
     3361 catcgccagg tgtatcccga tgtcgccgcg gcaattcgtg ccggcgaagt catgtccgcg
     3421 ttcgactcct attgccgcgg cggcttccgc acccgctcgc cgcattggct gttcgatgaa
     3481 ggctactacc gccgacgcta tcccgacatc accgatgccg ccctggagaa tgccgagctg
     3541 gccaacggct acgatcattt cctgcggcac ggcaatctgg aacagcgcat cggccatctg
     3601 ttcttcaatc ccggcctgta ctgtgccgat ctggaacccg atggcgcaga gcgggcgcgc
     3661 acgcagggtc ccttcctgca ctacctccgc cgcatccgcg cacgtgcgcc ggagccgcgc
     3721 accacggcgt atttcgatcc cgcctggtat ctcgcgcgct acaaggaggt ggccgaggca
     3781 atcgcggccg gcacctggat gtgcgccctg cagcattatc tgtgcaacga caccccgacc
     3841 gcgttcgatc cgctgccgga cttctccgaa ccctattacc tgacgcacaa ccacgatctc
     3901 gctcccgcga tcgggcgcgg cgacctgcgc aacggctaca tgcatttcct gctgttcggg
     3961 gccagggagc tgcgcgcccc cagcccggcg atcgacctgc actactatgc gcgccaggaa
     4021 ccggtccgcg ccgacctgga gcaagggctg gcgccggacg ccttctggca ctggctgcgc
     4081 atcggccgca gcaagggcct gaacgccgcg cagccgccgg aggagaccgt caccgaaggg
     4141 caggcgcgca cgctgtggcg ccgccgcggc caggccttgc tgccgctgct cgcgcgccat
     4201 ccgctggatt tcacctgcgc caccgccccg gtggtcagcg tggtgatgct gctgaaggac
     4261 gagttcgcgc aaacccttct gacgctagcc gccttacgtg ccagcttcgc cggcgccatc
     4321 gaactggtgc tgatcgatgc cggctctacc gacgaaacgc ggcatatcgg ccgctacgtc
     4381 cgcggtgcac agcttctgcc gctggagatg gacgtcgatc tggtcgccgg acgcaatgcc
     4441 gcgctgtacg cggtcaccgc ggaatacgtg ctgatgctgc agaccggggt cgagctggca
     4501 cccggcgcat tggctgcggc attgcggcgc atgcaggcgg aaccgtccgt cggcgcggtc
     4561 ggcgggcgca tgatcaagcc gcacggcatg ctggcggaag ccggctgcat cgtctggcat
     4621 gacggcacca cgctgtccta tctgcgcgat gccgatcctt tgcacccgga agccagcttc
     4681 cgccgcagcg tggatttctg cggatcgtcg tgcctgttgc tgcggaccgc gccgctgcgg
     4741 gatatcga
//
LOCUS       DASSZH010000549         2020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1598340, whole genome shotgun sequence.
ACCESSION   DASSZH010000549 DASSZH010000000
VERSION     DASSZH010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598340"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VFN42_11975"
     CDS             <1..499
                     /locus_tag="VFN42_11975"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8997374.1"
                     /translation="EQAHEFGLTPKQALSAPGFYLATAHAIGLEKAQGLLTMQSWYWD
                     SNAETRAWAQRFFARRQRMPNDVQAADYSAVGHYLRAVAKTGTAQGRDVIAAMRAAPV
                     KDVFTQDGTIRADNKMVFTRDLMRVKTPAQSHGPWDLLELVATVPPDQAFRPLNEGGC
                     PMVKT"
     gene            complement(539..850)
                     /locus_tag="VFN42_11980"
     CDS             complement(539..850)
                     /locus_tag="VFN42_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997375.1"
                     /translation="MMTSRVFAVLAAISLVAAFALATLLSPQLTLAQVIALFDARLLY
                     DSHEFVVAHGSDWLWGHMAMPLLLRPCWLVPTSLGLVFAGGAMTLGSRKGVTRSHRRR
                     S"
     gene            complement(847..1836)
                     /gene="msrP"
                     /locus_tag="VFN42_11985"
     CDS             complement(847..1836)
                     /gene="msrP"
                     /locus_tag="VFN42_11985"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HET8997376.1"
                     /translation="MFIRRRRGWEIPESRVTPEAAALGRRAALGAGLGAGAGLAALGT
                     ARAAHAWSLFDSGPSASPVPLRPLPARPNPRYVAGRKLTPADAAESYNNFYEFGESKS
                     VADEAQALRVAPWSIELAGMVRTPRTIGLDDLLKQVSLEERVYRHRCVEAWAMTVPWT
                     GFPLADLVRLAEPLGSATYLKFTTLADPKQMPGLHKPWYPWPYTEGLTMQEAMNELAF
                     LSVGLYGKTLPPQNGAPIRLTVPWKYGFKSAKSVVRVEFTDKRPETFWQRMQDSEYGF
                     WANVNPAVPHPRWSQAEERLIGSNETVPTQIWNGYGEFVAAMYADKTKERLFA"
BASE COUNT          335 a          710 c          660 g          315 t
ORIGIN      
        1 cgaacaggcg catgaattcg gcctgacccc caagcaggca ttgagcgcgc cgggcttcta
       61 tctggcgacc gcgcatgcga tcgggctgga gaaggcgcag ggcctgctca ccatgcagtc
      121 atggtactgg gacagcaatg cggaaacccg cgcctgggca caacgcttct tcgcccgccg
      181 ccagcgcatg cccaacgacg tgcaggccgc cgactactcc gcggtcgggc attatctgcg
      241 cgcggtggcg aagacgggca cggcgcaggg ccgcgacgtg atcgccgcca tgcgggcggc
      301 gccggtgaag gacgtgttca cccaggacgg caccatccgc gccgacaaca agatggtgtt
      361 cacccgcgac ctgatgcgcg tgaagacgcc ggcgcagtcg catgggccgt gggacctgct
      421 ggaactggtc gcgacggtgc cgccggatca ggcgttccgg ccattgaacg agggcggttg
      481 tccgatggtg aagacctgag gcgagcgcgc cccgttccgg gatgccgacg ggcgggcgct
      541 agctccgtcg gcggtgcgac cgcgtcacgc ccttgcggga accgagcgtc atggccccac
      601 cggcgaacac caggccgagc gaggtcggca ccagccagca cgggcgcagc agcagcggca
      661 tcgccatgtg tccccacagc cagtcggacc cgtgggccac cacgaattcg tgcgaatcat
      721 agagcaaccg cgcgtcgaac agggcaatca cctgtgccag cgtcagttgc ggcgacagca
      781 gggtggcgag cgcgaaggcc gccaccaggc tgatcgccgc cagcacggca aagacgcgcg
      841 atgtcatcac gcgaacagac gctccttggt cttgtcggca tacatggccg cgacgaactc
      901 cccgtagccg ttccagatct gcgtcggcac cgtctcgttg ctgccgatca gccgttcctc
      961 cgcctggctc cagcgcgggt gcggcacggc cgggttcaca ttggcccaga agccatactc
     1021 gctgtcctgc atcctctgcc agaacgtctc cggccgcttg tcggtgaact caacccgcac
     1081 gaccgacttg gcggatttga agccatactt ccacggcacg gtcaggcgga tcggagcgcc
     1141 gttctgcggc ggcagagtct tgccatacag gccgaccgac aggaaagcca gttcattcat
     1201 cgcctcctgc attgtcagcc cctccgtata gggccaagga taccacggct tgtgcagtcc
     1261 tggcatctgc ttcggatcgg ccagcgtggt gaacttcagg taggttgcgc tgcccagcgg
     1321 ctcggccagc ctgaccagat cggccagcgg gaacccggtc cacggcaccg tcatggccca
     1381 ggcctcgacg cagcgatgac gatagacgcg ctcctccagg ctgacctgct tcagcaggtc
     1441 gtcgagcccg atggtgcgcg gcgtcctgac catgccggcg agttcgatgc tccacggtgc
     1501 cacgcgcagg gcctgcgctt catcggccac cgacttgctc tcgccgaatt cgtagaagtt
     1561 gttgtagctc tcggcggcgt ccgccggcgt cagcttgcgg ccggcgacat agcgcgggtt
     1621 gggcctggcc gggagcggcc tgagcggcac cggcgatgcc gacggcccgc tgtcgaacag
     1681 gctccaggca tgcgcggcac gcgccgtgcc cagcgccgcg agccccgcgc ccgcgcccaa
     1741 tccggcgccg agtgccgcgc ggcgccccaa cgcggcggcc tccggcgtca cgcggctttc
     1801 cgggatctcc cagcctcgcc gtctgcggat gaacatctcc caggctcctt cctgctgtca
     1861 ctggaactac gtcccggcag acccggcgga tgttactgtg gggcgaagtc cgccgggcag
     1921 caatggccgg catttgaccc cgctccttcg cccgcgcagg atggcggcat gatcctggtc
     1981 atcgacaact acgacagctt cacgttcaac ctcgtccact
//
LOCUS       DASSZH010000550         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1445081, whole genome shotgun sequence.
ACCESSION   DASSZH010000550 DASSZH010000000
VERSION     DASSZH010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445081"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..955)
                     /locus_tag="VFN42_11990"
     CDS             complement(173..955)
                     /locus_tag="VFN42_11990"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET8997377.1"
                     /translation="MSEPETLTVDVNGFASRVWRKGSGPKLGFLAGFGGLPRWVPFLD
                     ELAKSRTVIVPSVPGFPGGDRGHTVLDTHLDWLLAVHELLEKAGLSGADLAGSSVGGS
                     LAAEMAALWPASVRRLALIGPWGVYDEADPPTDPWAQRGDAVPGLMCAEPAIWSALKA
                     VPEGANSVEWPIEQVRAAEAAARIFWPLGNTKLEKRLRLIQAPTLLLWGEQDRIMPRG
                     YAERFAKGIAGKTETQVIAGAGHLAELDKPADVAAAILRWTA"
     gene            complement(1098..>2238)
                     /locus_tag="VFN42_11995"
     CDS             complement(1098..>2238)
                     /locus_tag="VFN42_11995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8997378.1"
                     /translation="DELEYAASCGFDAVCVNEHHSNGYGLMPSPNLIASSLARRTTDT
                     AICVMGNSLALYNPPTRVAEEFAMIDCISGGRLIAGFPVGTPMDTCYAYGTNPSQLRQ
                     RYLEAHDLVLRAWKEKDTFAFNGRFNQQRYVNIWPRPVQNPHPPIWIPGGGSVETWHW
                     CAEMDYVYCYLSYYGYKAGQATMTGFWNEMSRLGKDRNPYRAGFLQFVGVGESRQHAF
                     DLYAEAAEYFYGRCLHVDARWATPPGYTSEGSQRAGIESQVKQAADANVRGKSTGERM
                     ASVAREMNGIVDNGYVIIGSPDEVAEQLREVAVNLNVGHLMLLLQFGNMSKDLTRYNT
                     KLFAEQVMPKLKGQFDEWEDRWWPQPMAQTLRAAVPAYTPRIAAE"
BASE COUNT          345 a          762 c          743 g          388 t
ORIGIN      
        1 aggtcgcgcg tctcggcgac cggcgccgga tcgggctgcg ttacggttcg gccggcacgg
       61 aaggcctcgc gtgcctcgcc gggcgtcagc gtctcgaatg gcggacggcc cgccgcccgc
      121 atcatctcca gcaggcgttc ggcgtcggga tcgagcggca tggcggtttc cttcaggcgg
      181 tccagcgcag gatggcagcc gcgacgtcgg ccggtttgtc gagttcggca agatggccgg
      241 cgccggcgat cacctgggtt tccgttttgc cggcgatccc tttggcgaac cgctccgcat
      301 agccgcgtgg catgatgcgg tcctgctcgc cccagagcag cagcgtgggc gcctggatca
      361 gccgcagccg tttctccagt ttggtgttgc ccagcggcca gaagatgcgg gcagcggctt
      421 cggcggcgcg cacctgctcg atcggccatt ccacggagtt ggcgccctcc ggcaccgcct
      481 tcaatgcact ccagatggcc ggctcggcac acatcaggcc gggtacagcg tcgccgcgct
      541 gcgcccaggg atcggtgggc ggatcggctt cgtcatacac cccccaggga ccgatcaatg
      601 ccaggcgccg cacggaggcg ggccacagcg ctgccatttc cgccgccagg gaaccgccga
      661 ccgaactgcc ggcgagatcg gcgccgctca gcccggcctt ctccagcaat tcgtgcaccg
      721 cgagcagcca gtcgagatgc gtgtccagca cggtgtggcc gcggtcgccg ccggggaatc
      781 cgggcaccga cggcacgatc accgtgcgcg atttcgccag ttcgtcgagg aacggcaccc
      841 agcgcggcag tccgccgaag ccggcgagaa agcccagctt ggggccgctg cccttgcgcc
      901 agacgcggga ggcaaagccg ttgacatcga cggtcaacgt ttcgggttca ctcacggttc
      961 tgctccggtt gattcggaag actccccctc cccttgcggg agggggttgg gggagggggc
     1021 ctgcatgcgt agcctgggca acctcgaccg ccccctcccc caccccctcc cgcaagggga
     1081 gggggagtcg gtattgctta ttccgcggcg atccgtggcg tgtatgccgg caccgcggcg
     1141 cgcagcgtct gcgccatcgg ctgcggccac cagcgatcct cccactcatc gaactgtccc
     1201 ttcagcttcg gcatcacctg ctcggcgaac agcttcgtgt tgtaccgtgt gaggtccttg
     1261 ctcatgttgc cgaactgcag cagcagcatc aggtggccga cattcaggtt caccgccact
     1321 tcgcgcaact gctccgcgac ctcatccggc gaaccgatga taacgtagcc gttgtcgacg
     1381 atgccgttca tctcgcgtgc gacggaggcc atgcgctcgc cggtggattt gccgcgcaca
     1441 ttggcatccg cggcctgctt cacctggctt tcgatgccgg cgcgctggct gccctcactg
     1501 gtatagcccg gcggcgttgc ccagcgggcg tccacgtgca ggcagcggcc gtagaaatac
     1561 tccgcggcct cggcgtaaag atcgaaggcg tgctggcggg attcgccgac gccgacgaac
     1621 tgcaggaagc cggcgcggta tgggttgcgg tccttgccca gccgcgacat ctcgttccag
     1681 aagcccgtca tcgtcgcctg gccggccttg tagccgtagt acgacaggta gcagtacacg
     1741 tagtccatct cggcgcacca gtgccacgtc tccaccgagc cgccgccggg aatccagatc
     1801 ggcggatgcg ggttctgcac cgggcgcggc cagatgttga catagcgctg ctggttgaag
     1861 cggccgttga aggcgaaggt gtccttctcc ttccaggcgc gcagcaccag gtcatgtgct
     1921 tccagatagc gctggcgcag ctggctcggg ttggtgccgt aggcgtagca ggtgtccatc
     1981 ggcgtgccga ccggaaaccc ggcaatcagg cgtccgcccg agatgcagtc gatcatggcg
     2041 aattcttccg ccacgcgggt cggcggattg tacagcgcca gcgaattgcc catcacgcag
     2101 atcgcggtat cggtggtgcg ccgcgccagc gacgacgcaa tcaggttggg cgacggcatc
     2161 agcccgtagc cgttggaatg atgttcgttc acgcacaccg catcgaaacc gcaggacgcc
     2221 gcatattcca gctcgtcc
//
LOCUS       DASSZH010000551         3502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_115469, whole genome shotgun sequence.
ACCESSION   DASSZH010000551 DASSZH010000000
VERSION     DASSZH010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115469"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(936..1631)
                     /gene="rplA"
                     /locus_tag="VFN42_12000"
     CDS             complement(936..1631)
                     /gene="rplA"
                     /locus_tag="VFN42_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HET8997379.1"
                     /translation="MAKVGKRLKAARAAVQRGKLYPLADAIKLLKAAPKAKFDETIEM
                     SMNLGIDPRHADQMVRGLTNLPNGTGKSVRVGVFARGAKAEEAQAAGADVVGAEDLAE
                     KIQAGEINFDRCIATPDMMGVVGRLGKVLGPRGLMPNPRLGTVTMDVKGAIAAAKAGQ
                     VEFRAEKAGIIHAGIGKVSFDEDKLVENARALADAIQKARPAGAKGTYLKSAALSSTM
                     GPGVRLDVSTLNA"
     gene            complement(1636..2070)
                     /gene="rplK"
                     /locus_tag="VFN42_12005"
     CDS             complement(1636..2070)
                     /gene="rplK"
                     /locus_tag="VFN42_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014105037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HET8997380.1"
                     /translation="MAKKIVGYIKLQIPAGKANPSPPVGPALGQRGLNIMNFVKEFNA
                     VTANMEPGMPIPVVITAYADRSFTFITKTPPNTYFLKKAAKIDKGSQTVGKGASVGSV
                     TMAQLREIAEQKMKDMNANDVDGAVRMLIGSARSMGLTVVEG"
     gene            complement(2228..2431)
                     /locus_tag="VFN42_12010"
     CDS             complement(2228..2431)
                     /locus_tag="VFN42_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997381.1"
                     /translation="MYVIAESPIDAIRADFRRIQESSVLHRIWHALATHSERVADRAA
                     ARSIRRVAHPGVTADHAAARACR"
     gene            complement(2538..>3502)
                     /locus_tag="VFN42_12015"
     CDS             complement(2538..>3502)
                     /locus_tag="VFN42_12015"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HET8997382.1"
                     /translation="RRPAMSVPNRCKFLMLPPHTPITRDWAARLAAGVPEMQVVVAED
                     ADAAFGWLNPELLARAKRLRWLQAPQAAPAAGYYFPELIAHPLVVTNFREIFNDHISA
                     HIMAFVLAFARGLHVYIPQQLRHEWKKPPEDQGVIHLPEATALLVGAGGIGAETARLL
                     AAFGVTVLATDARRTAPPDGVAELHPPGALDSLLPRADFVILTVPHTPATEGLFHRAR
                     FQRMKPGAFFINIGRGKTTRLDDLVAALLAGEIAGAALDVFEQEPLPEDHPLWTMPNV
                     LLTPHMAGYGPYLNDRRLQIILDNARAFAAGRPLRNLVDKANWF"
BASE COUNT          541 a         1192 c         1179 g          590 t
ORIGIN      
        1 ctgggatgcg ctcagccgtt tgcaccagaa gggcatgatc ggcaatccgg tcggcaaggc
       61 aaaatcggtg gtgttcaccg aggaaggcct ggccgaaagc gcgcgactgt tccgaaagtt
      121 attcgagaag ccggcgggat agcggcagat cacccctgag agcaactgtt cttgctgtcc
      181 cgttcagccc gccagcggcc ggccggcgta cgggcggccg ccacgccgcc ggattgacat
      241 taataataac agacattata ctaactatca tgacccatac cctctccccc gacccaaccc
      301 agcgtctcta ccacggctac tatgaggtcc tgcacaaagc cctccagatc ctgccgccgc
      361 cgctggagga aaccccggaa ggccacgccc gccgcaatgc cgccgtggcg gaccagatcg
      421 ccttgctggt ccccgccagc gccgtggagg cagagctcgc cgccaagtgc ggcgccggct
      481 cggaacgctg gatcgagtgc ctccgcgcag ccaacacata cggcgcagcg gctgcgctgt
      541 gcgtgacgga aggaaatgga acgcaaatgg ggggaatgcg gccaagccgc gggcggcggc
      601 ccttcgcccg ccatggcggg gacggcgcca gcattcatgc aggttggaat aatggatgga
      661 ctatccggac ccgagtcgca tcagaaccga cggcgccgga ccatccgcgt tcatctgcgg
      721 ctgttcgcgt tcatctgcgt catggtcttg ttctccaccc gccgcggacg gacaaaatcc
      781 ggcaagactt cccccggcac cgaccgcgcc acgcaccttc ccaggcgcag cggatcactc
      841 caggagacgt catcccgatt aacgggcaat gagacagccg cgagacgccc gctcggcgcg
      901 gggtcacggc cgttcccggc catgaccccg gcgggctacg cgttcagcgt ggagacatcc
      961 agccgcacgc ccggccccat ggtggagctc agcgcggcgc tcttcaggta cgtgcccttc
     1021 gcccccgccg gacgcgcctt ctggatggcg tcggccaaag cgcgggcgtt ctccaccagc
     1081 ttgtcctcgt cgaagctgac cttgccgata ccggcatgga tgatgcccgc cttctcggcg
     1141 cggaactcca cctggccggc cttggcggcg gcaatggcgc ccttcacgtc catcgtcacg
     1201 gtgcccaggc gcgggttcgg catcaggccg cgcgggccca gcaccttacc cagccggccg
     1261 accacgccca tcatgtccgg cgtggcgatg cagcggtcga aattgatctc gccggcctgg
     1321 atcttctcgg ccaggtcttc cgcgcccacc acgtcggcgc cggcggcctg ggcctcctcg
     1381 gccttggcgc cacgggcgaa cacgcccacc cgcaccgact tgccggtgcc gttcggcagg
     1441 ttggtcaggc cgcgcaccat ctggtcggca tggcgcgggt cgatgcccag gttcatcgac
     1501 atctcgatgg tctcgtcgaa cttcgccttg ggcgccgcct tcagcagctt tatggcgtcg
     1561 gccagcggat acagcttgcc ccgctgcacg gcggcgcggg cggccttcag gcgctttcct
     1621 acctttgcca tgggatcagc cctccaccac ggtcaggccc atcgaccggg cggaaccgat
     1681 cagcatgcgc acggcgccgt ccacgtcgtt ggcgttcatg tccttcatct tctgttcggc
     1741 gatttcccgc agctgcgcca tggtcacgct gccgacggaa gcccccttcc ccacagtctg
     1801 gctgcccttg tcgatcttgg cggccttctt caggaaatac gtgttgggcg gggtcttggt
     1861 gatgaaggtg aagctgcggt ccgcataggc ggtgatcacc accgggatcg gcatgcccgg
     1921 ttccatgttc gcggtgaccg cattgaactc cttgacgaag ttcatgatgt tcaggccgcg
     1981 ctggcccagc gccgggccga ccggcggcga gggattcgcc ttgccggcag gtatctgcag
     2041 cttgatgtag ccgacgatct tctttgccat ccctggtcct ttccgtgtcc cggggaccgc
     2101 ggtgcaaacg gcgcgcctcc gcccgggatc ggggcggtac gcgtctcccg cgttccttgt
     2161 gaagcggcgg cgtgtagccg aaagcgctcg gtccgtccag cccggcggcg gcccgcttca
     2221 ggaccggtca gcggcaggcg cgggctgccg cgtgatcggc cgtcacgccg ggatgcgcca
     2281 cccggcggat gctgcgggcg gcggcgcgat cggcgacgcg ctcgctgtgg gtggcgaggg
     2341 catgccagat gcgatgaagc accgagcttt cctggatccg gcggaagtct gcgcggatgg
     2401 cgtcgatcgg ggattcggcg atgacataca tggaacgacc tccctggccg gttgtcctgt
     2461 ggccgggaga ctgtgcgact tgcggcggat tgacagtgaa ctgcgccaca tccggcagcg
     2521 attttcacgt tgccgggtca gaaccagttg gccttgtcca ccaggttgcg caatggcctc
     2581 ccggcggcga aggcgcgggc attgtccagg atgatctgca gccgccggtc gttcaggtac
     2641 ggaccatagc cggccatgtg cggcgtcagc agcacattgg gcatggtcca tagcggatgg
     2701 tcctccggca gcggttcctg ctcgaacacg tccagcgcgg caccggcgat ctcgccggcg
     2761 agcagcgcgg cgaccagatc gtccaggcgg gttgtcttgc cgcggccgat attgatgaag
     2821 aacgcgccgg gcttcatgcg ctggaagcgg gcgcggtgga acagcccttc ggtggccggc
     2881 gtgtgcggca cggtcaggat cacgaaatcc gcacgcggca gcagcgagtc cagcgccccc
     2941 ggcgggtgca gttcggcgac gccgtccggg ggggcggtgc gccgtgcatc ggtggccagt
     3001 acggtgacgc cgaaggcggc gagcaggcgt gcggtctccg cgccgatgcc gcccgcgccg
     3061 accagcagcg cggtggcttc tggcaggtgg atgacgccct ggtcctccgg cggcttcttc
     3121 cattcgtggc gcagttgctg cgggatgtag acatgcaggc cgcgggcgaa ggccagcacg
     3181 aaggccatga tgtgtgcgct gatatggtcg ttgaagatct cccggaagtt ggtcaccacc
     3241 aacgggtggg cgatcagttc cgggaagtag tacccggcgg ccggtgcggc ctgcggcgcc
     3301 tgcagccagc gcagccgctt ggcccgcgcc agcagctccg ggttcagcca gccgaacgcc
     3361 gcatcggcgt cctccgccac gaccacctgc atttcgggca cccccgcggc cagccgagcc
     3421 gcccagtcgc gggtgatcgg ggtgtgcggc ggcagcatga ggaacttgca gcggttcggc
     3481 acggacatgg cgggcctcct tc
//
LOCUS       DASSZH010000552         3741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_81437, whole genome shotgun sequence.
ACCESSION   DASSZH010000552 DASSZH010000000
VERSION     DASSZH010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81437"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..922)
                     /locus_tag="VFN42_12020"
     CDS             complement(101..922)
                     /locus_tag="VFN42_12020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8997383.1"
                     /translation="MPEQRKVAIVTGAASGLGRAMTLGLLGAGINVAAVDRNAALLDK
                     LGSDAAGRPGAVQTIAADLAQPDAFESIVAEVLRGHGRIDVLVNNAGIGQAALRADQR
                     RQPIRFWEVTPEQWSRFVAVNATAPIMMARAVLPHMLQAGQGRIISVTTSLGTMVRGG
                     YLLYGASKAAAEAAMAGLSADLAGTGVTSNVLVPGGVTNTPLVGDDAGDRSRMLQPEI
                     MVPPLLWLVSDAAAGVNGRRFIAADWDAALPPAKAAEHAGAPIAWMSIARMPIEP"
     gene            1064..2269
                     /locus_tag="VFN42_12025"
     CDS             1064..2269
                     /locus_tag="VFN42_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET8997384.1"
                     /translation="MDLRFTPEETAFREEVRAFIRDNLPTDIRDRMRLGHSPRKEDTV
                     RWQRILNQKGWAGLSWPKEWGGPGWSAIQKMIFLEENQVAPAPELLSFNITMIGPVLI
                     QFGTEEQKKRFLPRAANLDDWWCQGFSEPGAGSDLASLKTAAKLDGDHYVVNGQKIWT
                     STAHNADWIFLLVRTNPNAAKRQEGISFLLVDMKTPGITVRPIISIDGSHHLNEVFFD
                     DVKVPVSMRVHQENKGWDVAKFLLGNERTGIARLGKSKERVKAAKEMAREVNLHGRPL
                     ITDPTFRQRVAQMEVDMKALEITQYRVVSAYDKMKGAGRPDPLSSVLKVKGTELLQAT
                     TELAMDVAGPLSMPVWAQELAAMNEPDEMMEAASAATGSYLFLRAASIYGGTNEIQKN
                     ILTKAVLGL"
     gene            2402..3538
                     /locus_tag="VFN42_12030"
     CDS             2402..3538
                     /locus_tag="VFN42_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET8997385.1"
                     /translation="MDFELSDEQRLLQDSVSRLLSDRYAFEQRKTYLKDQAGFSPALW
                     SQYAELGLLGLPFAEEYGGFGGGAQEVMLVMQAFGRALILEPYLSTVVLGGTALQLAG
                     SDAQKNALLPTLAEGGLKLAFAHGERQARYDLNDIVTTAKRGNGGWVLDGAKSVVSHG
                     ETADRLVVSARTSGDRHDESGITLFLVDANASGVAKRGYAARDETRAADIAFSNVSVA
                     DDDVLGEVGGGFGIVQRVVEAGIAATAAEAVGAMEVMHSMTLEYSKTREQFGKPIGSY
                     QVVQHRLADMFMSMEQGRSMAMLATMSLEQPDAAERERSMAMAKVGVGQAARYVAQSA
                     VQMHGGIGMTEEYAVGHYFRRCMAIERLWGDPAYYLQKLAEDVE"
BASE COUNT          649 a         1275 c         1264 g          553 t
ORIGIN      
        1 ccggctgaca cccacagagt tgtccacagg ggcaacccgc gttccgggag gcactcgcga
       61 tccgatgcgg cggcaccgaa tcgatccagc cgtccggtct tcagggctcg atcggcatcc
      121 gggcaatgct catccaggcg atcggcgcac ccgcgtgttc cgccgccttg gccggtggca
      181 aggccgcatc ccaatccgcg gcgatgaacc ggcgtccgtt caccccggcc gccgcatccg
      241 acaccagcca gagcaacggc ggcaccatga tctccggttg cagcatgcgg ctgcggtcgc
      301 cggcatcgtc gcccaccagc ggcgtgttgg tcactccgcc cggcaccagc acgttcgagg
      361 tcacgcccgt gccggcgagg tcggccgaca gcccggccat cgccgcttcc gccgcggcct
      421 tgctggcgcc gtacagcagg tagccgccgc gcaccatggt tcccaggctg gtggtgacgc
      481 tgatgatccg gccctggccg gcttgcagca tgtgcggcag cacggcccgc gccatcatga
      541 tcggcgcggt ggcattcacc gcgacgaaac ggctccactg ctcgggcgtc acctcccaga
      601 accggatcgg ctgccggcgc tgatcggcac gaagcgcggc ctggccgatc cccgcattgt
      661 tcaccagtac gtcgatgcgg ccgtgccccc gcaacacctc cgcgacgatg ctttcgaacg
      721 catccggctg cgccaggtcg gcggcaatgg tctgcaccgc gcccggccgt cccgcggcat
      781 cgctccccag cttgtccagc agggccgcgt tgcggtccac cgccgcgacg ttgatccccg
      841 cgcccagcag acccagcgtc atcgctcggc cgagcccgct cgccgcgccg gttacgatgg
      901 cgaccttgcg ttgctccggc atgttcgttc ctcccaatcc gcgctgcggt gaagcttcac
      961 agaatccggg aaggccggcc agtggtctcc ccgattggtc tcccccgatt gcaccgccgc
     1021 cgcaccggcg cttaagctac cggcaagtag gaggacccga tccatggatc tgcgcttcac
     1081 cccggaagaa accgcgttcc gcgaagaagt ccgcgctttc atccgcgaca acctgcccac
     1141 cgacatccgc gaccgcatgc gtctcggcca cagcccgcgc aaggaagaca ccgtgcgctg
     1201 gcagcgcatc ctcaaccaga agggctgggc cgggctgtcc tggccgaagg aatggggcgg
     1261 tccgggctgg agcgcgatcc agaagatgat cttcctggag gaaaaccagg tcgcccccgc
     1321 gccggaactg ctgtctttca acatcaccat gatcggcccg gtgctgatcc agttcggcac
     1381 cgaggagcag aagaagcggt tcctgccccg cgccgccaac ctggacgact ggtggtgcca
     1441 gggcttctcc gagccgggcg ccggttccga cctggcctcg ctgaagaccg ccgcgaagct
     1501 ggatggcgac cattacgtcg tgaacggcca gaagatctgg acctccaccg cgcacaacgc
     1561 cgactggatc ttcctgctgg tgcgcaccaa cccgaacgcc gccaagcggc aggagggcat
     1621 ctccttcctg ctggtcgaca tgaagacgcc cggcatcacc gtgcggccga tcatctcgat
     1681 cgacggcagc caccacctga acgaagtgtt cttcgacgac gtgaaggtgc cggtgagcat
     1741 gcgcgtgcac caggagaaca agggctggga cgtggccaag ttcctgctgg gcaacgagcg
     1801 caccggcatc gcccggctgg gcaagtcgaa ggagcgggtg aaggccgcga aggagatggc
     1861 gcgggaggtg aacctgcacg gccgtccgct gatcaccgat cccaccttcc gccagcgtgt
     1921 cgcgcagatg gaagtggaca tgaaggcgtt ggagatcacc cagtaccgcg tggtgtccgc
     1981 ctacgacaag atgaagggcg ccggccggcc ggatccgctg tcctcggtgc tgaaggtgaa
     2041 gggcaccgag ctgctgcagg ccaccacgga actggcgatg gatgtcgccg gaccgctgtc
     2101 gatgccggtc tgggcgcagg aactggcggc gatgaacgag ccggacgaga tgatggaagc
     2161 cgccagcgcg gccaccggca gctacctgtt cctgcgcgcc gcctcgatct acggcggcac
     2221 caacgagatc cagaagaaca tcctgaccaa ggccgtgctg ggactctaag cggccgggcg
     2281 cggcccgctc tcctcgtgtc gtggccggcg aaggccggcc atccacgacc tgtcggagtg
     2341 gccacagcaa agacgtgggt gcccgggcca agcccgggca tgacacaggg aagaggcaac
     2401 catggacttc gaactttccg acgaacaacg gctgttgcag gactcggtct cccggctgct
     2461 gtccgaccgc tacgctttcg aacagcgcaa gacgtacctg aaggaccagg ccggcttcag
     2521 ccccgcgctt tggtcgcaat acgccgagct cggcctgctc ggcttgccct tcgccgagga
     2581 gtacggcggc ttcggcggcg gcgcgcagga ggtgatgctg gtgatgcagg ccttcggccg
     2641 cgcgctgatc ctggagccgt acctgtccac cgtggtgctg ggcggcactg cgctgcaact
     2701 cgccggcagc gatgcgcaga agaacgcgct gctgcccacg ctcgccgaag gcggcctgaa
     2761 gctggccttc gcacacggcg agcggcaggc gcgctacgac ctgaacgaca tcgtcaccac
     2821 ggcgaagcgc ggcaacggcg gctgggtgct ggacggcgcc aagagcgtgg tgtcgcacgg
     2881 cgagacggcg gatcggctgg tggtcagcgc ccgcacctcc ggcgaccgcc acgacgagag
     2941 cggcatcacg ctgttcctgg tggatgcgaa tgcctccggc gtggcgaagc gcggctacgc
     3001 ggcacgtgac gagacgcgcg cggcggacat cgccttctcc aacgtgtcgg tggcggatga
     3061 cgacgtgctg ggcgaggtcg gcggcggctt cggcatcgtg cagcgcgtgg tggaggccgg
     3121 cattgcggcg accgcggcgg aggcggtggg cgcgatggaa gtcatgcaca gcatgacgct
     3181 tgagtacagc aaaacccggg aacagttcgg caagccgatc ggctcctacc aggtggtgca
     3241 gcaccggctg gcggacatgt tcatgagcat ggagcaggga cgcagcatgg cgatgctcgc
     3301 caccatgtcg ctggagcagc cggacgcggc ggagcgcgag cgcagcatgg ccatggcgaa
     3361 ggtcggcgtg ggccaggccg cgcgctatgt ggcgcaaagc gcggtgcaga tgcatggcgg
     3421 catcggcatg accgaggaat acgccgtcgg ccactatttc cgccgctgca tggcgatcga
     3481 gcggctctgg ggcgacccgg cctactatct gcagaagctg gcggaagacg tcgaatagca
     3541 gttgtcacgt gtcacggccg atgtgcgcac ccccgtgtca tggccggcgc aggccggcca
     3601 cccacgcctt gctgcgggaa acgccgaacg cacggatcac tgcttcgagt cccctccagc
     3661 aagtcgtgtt ggtgtggacg gccccacggc atcgggtgcc aaagttgggt tgtcactgaa
     3721 acacaccaac aaggaggccg t
//
LOCUS       DASSZH010000553         6247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1530676, whole genome shotgun sequence.
ACCESSION   DASSZH010000553 DASSZH010000000
VERSION     DASSZH010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530676"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="VFN42_12035"
     CDS             <1..848
                     /locus_tag="VFN42_12035"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HET8997386.1"
                     /translation="LRRLGFGFVETGTVTPRPQQGNPRPRLFRLLPDDAVINRMGFNN
                     GGLEAYLRRLAAVPRSDIPLGANVGINKEGADPPRDYPALIAAVAPHADYTVINVSSP
                     NTPGLRDLQGEQQLRAILQAVAAVPNRPPLLVKIAPDLSEPALAAIVETCVAEGVQGL
                     IVSNTTISRPPGLHAPQARETGGLSGRPLFALSTTVLARAFRLARGRLVLIGVGGVLS
                     GRDALAKIQAGASLVQLYTGFALHGPALIPWLKAELAQALAEAGFARVEDAVGTRAAH
                     FAGQD"
     gene            849..1715
                     /locus_tag="VFN42_12040"
     CDS             849..1715
                     /locus_tag="VFN42_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HET8997387.1"
                     /translation="MEHLSGFAALADRYDGFVLDLWGVIHDGVNAFPHAVDSLRHLRA
                     AGKRTLLLSNVPRPNQAVQVMMRGMGIEDSLYTDLLTSGEAVRRALQNPPNLWWAELG
                     QRVFHLGPVRDLPVLEDLPLRRVPDPAEADFVLNTGPDDHRNPTDMAEFEPVLQECWR
                     HRLPMICANPDLEVIRGGKRVLCAGALAVRYRALGGDVQSLGKPDPAIYQPVLQQLDL
                     PPDRVLAVGDALRTDIAGASAVDLPSCWVLDGIHGAELRDSAGGFDTARAAEMARAEG
                     LMPVATIPRFVW"
     gene            complement(1747..2709)
                     /locus_tag="VFN42_12045"
     CDS             complement(1747..2709)
                     /locus_tag="VFN42_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HET8997388.1"
                     /translation="MGQPIPPPLPHFDVRLTAPQIGDWIPGNIGVPGFTTRDSGKPGP
                     HVAILGLSHGNEIAGGIVLDRLLRSGLAPRRGRLTFGFVNLAAFARFDPRHPTLSRFV
                     DEDLNRVWDPAVLDGPRKSSELERAREIRPVIDTVDTLLDLHSMLWPSEPLMLCGATE
                     KGRALARGIGAPPLVVADHGHVSGKRIIDYDRFGSPDTPYAANLVEAGQHWQPGTVDT
                     MLACVAGLLRHLDMAEHPALPASVPPRPQRVAEVTTAVTASTSGFAFVQPWHGGEVVA
                     RRNTLIALDGASEIRTPYDSCMLVMPSLRPSRGHTAVRLARFVG"
     gene            complement(2726..3901)
                     /gene="argE"
                     /locus_tag="VFN42_12050"
     CDS             complement(2726..3901)
                     /gene="argE"
                     /locus_tag="VFN42_12050"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007281926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HET8997389.1"
                     /translation="MNAPNSAPRMSAAELLARLVAFDTTSRNSNLKLIAFVRGYLDAH
                     GVPYRISTDPAGQKANIHAIIGPQTAGGVALSGHVDTVPVDGQAWSADPFTLRQADGK
                     LYARGACDMKGFVASCLAAVPDFLARKPVKPLHLFISYNEEVDFQGAIRLIEDLDESG
                     LKPALCVVGEPSGMKPILAHKGKLDVRVKVRGLPGHSSVPAKGVNAVQAAAEAIAWVA
                     AEARRLEKEGPFEDGFDPPHTTIHVGTVQGGSILNIIPEHAEFMMEWRNIPGDDAEAA
                     LERMQVHIANHIEPAMRAVNPNCGFSYEVPVRMPGLALGADHELTAIVKQLTGSNDTG
                     KVSYGTEGGFYQNAGIATIICGPGHIAQAHQPDEWLAQDQLDACDAFIRRLADRLLT"
     gene            4128..4961
                     /locus_tag="VFN42_12055"
     CDS             4128..4961
                     /locus_tag="VFN42_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET8997390.1"
                     /translation="MPFLTEPTPERGIILPVLPGISRIVADNPGPMTYYGTNTYLIET
                     DDGMVVLDPGPEDHPEHVEAIMRATGGAVALILVSHTHHDHVGAVPALQDATGAPTVG
                     FQESMLDSFDADIKLADGDTIAGMQALHTPGHASDHLCFSLAASDGTAVLFSADHVMS
                     WSTSVVSPPGGDMAAYFSSLRLLLDRTDDVYLPGHGPPLPEPRELVREMLHHRMARER
                     AIATRLQEGPADTFVLMDTLYSQVHPRLRRAAERNVLAHLLKMELEGKVVRDGDLWRA
                     A"
     gene            5011..6213
                     /locus_tag="VFN42_12060"
     CDS             5011..6213
                     /locus_tag="VFN42_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HET8997391.1"
                     /translation="MTQPQIDSARLLADLHALRGIGSYKTGAHRPTYSPQDMQSRHWL
                     ADRMTQAGLTPEIDGVGNVIGRAAGPGPHLLLGSHIETQPHAGWLDGALGVIYGLEVA
                     RALGRNIDVAAWADEEGHFDNFVGSRSFTGNLTEADIDRARNRYDGTPLRNALQAAGL
                     AGRPRAQVDPARYRGYLEAHIEQGDELDTSGKRLGVVTSIVAIYQYRIRFHGVQNHAG
                     TTRMAIRKDAGLALVRLAAAIDRRFPEVAGPRSVWTTGRIALEPGAASIVPGGAEMMF
                     QFRDADPQRLAVLHQTLEQLVAEANAGPCRVELQVMSQSTPKVMDAGFQDALAAAAEA
                     RAPGNWVRMPSGAGHDAQVLAQAMPAGMLFVPSIGGISHHWSEDTKEEDIVLGCQVLT
                     DAAAALLA"
BASE COUNT          941 a         2176 c         2158 g          972 t
ORIGIN      
        1 gattgcgccg gctcggcttc ggcttcgtgg aaaccggcac cgtcaccccg cgtccgcagc
       61 aaggcaatcc gcgtccccgc ctgttccgcc tgctgccgga cgacgcggtg atcaaccgca
      121 tgggcttcaa caacggcggg ctggaggcgt atctccggcg cctcgccgcg gtgccgcgca
      181 gcgacatccc gctgggcgcc aatgtcggga tcaacaagga aggcgccgac ccgccgcgcg
      241 actacccggc gctgatcgcc gccgtggcgc cgcatgccga ttacacggtg atcaacgtgt
      301 cgtcgccgaa cacccccggg ctgcgcgacc tgcaaggcga acagcagctc cgcgctattc
      361 tgcaggcggt ggcggccgtg ccgaaccgcc cgcccctgct ggtgaagatc gcccccgacc
      421 tgtcggagcc ggcgctcgcc gcgatcgtgg agacctgcgt cgccgagggc gtgcaggggc
      481 tgatcgtctc caacaccacg atcagccgcc cgcccgggct gcacgcgccg caggcaaggg
      541 aaaccggcgg attgtctggg cggccgctat tcgcgctgtc caccacggtc ctggcccgcg
      601 ccttccggct ggcgcgcggc cggctggtcc ttatcggcgt gggcggcgtg ctctccggcc
      661 gcgacgccct ggccaagatc caggccggcg cttcactggt gcagctctac accggcttcg
      721 ccctgcacgg tcccgcgctg atcccatggc tgaaggccga actggcgcag gcgctggctg
      781 aggccgggtt tgcccgggta gaggatgcgg tcgggaccag ggcagctcac ttcgcaggac
      841 aggactgaat ggaacatctc tccggcttcg cggcgctggc ggaccgctac gacgggttcg
      901 tgctcgacct gtggggcgtg atccatgatg gcgtgaacgc gttcccacac gcggtggact
      961 cgctccgcca cctgcgcgcg gccggcaagc gcacgctgct gctgtccaac gtgccgcggc
     1021 cgaaccaggc ggtgcaggtc atgatgcgcg gcatgggcat tgaggactcg ctgtacaccg
     1081 acctgctgac cagcggcgag gcggtgcggc gtgcgctaca gaatccgccc aacctgtggt
     1141 gggccgagct ggggcagcgc gtgttccacc tggggccggt gcgtgacctg ccggtgctgg
     1201 aagacctgcc gctgcggcgc gtgccggatc cggccgaggc ggatttcgtg ctgaacaccg
     1261 ggccggacga tcatcgcaat ccgaccgaca tggccgagtt cgagccggtg ctgcaggaat
     1321 gctggcgcca ccgcctgccg atgatctgcg ccaacccgga tctggaagtc atccgcggcg
     1381 gcaaacgcgt gctgtgcgcc ggggcgttgg cggtgcgcta tcgcgcgctg ggtggcgacg
     1441 tgcagtcgct gggcaagccc gatccggcga tctatcagcc agtgctgcag cagctcgatc
     1501 tgccgccgga ccgcgtgctg gcggtgggcg acgcgttgcg caccgacatt gcgggagcaa
     1561 gtgcggtcga tctgccgtcc tgctgggtgc tggacggcat ccatggcgcg gagctgcgcg
     1621 acagtgcggg cggcttcgat accgcgcgtg ccgccgagat ggcccgcgcc gaggggctga
     1681 tgccggtcgc gaccattccg cgcttcgtct ggtagccgcc gcggcaccgg ggagcccggc
     1741 gccgcatcac cccacgaagc gggccagccg caccgcggta tgtccgcggc ttggccgcag
     1801 gctcggcatc accagcatgc aggaatcgta cggggtgcgg atctcgctgg cgccgtccag
     1861 cgcgatcagc gtgttgcgcc gagcgaccac ttcgccgcca tgccagggct ggacgaaggc
     1921 gaagccgctg gtggaggcgg tgactgccgt cgtcacctcc gccacgcgct gcggccgcgg
     1981 cggcaccgag gccggcagcg ccggatgctc cgccatgtcc agatgccgca gcagcccggc
     2041 cacgcaggcc agcatggtgt ccaccgtgcc gggctgccag tgctggccgg cttccaccag
     2101 gttcgccgca tagggcgtgt ccggcgagcc gaaccggtcg tagtcgatga tgcgcttgcc
     2161 gctgacatgc ccatggtcgg ccaccaccag cggcggcgcg ccaatgccgc gggccagcgc
     2221 ccggcccttt tcggtggcgc cgcacagcat cagcggctcc gacggccaca gcatggaatg
     2281 caggtccagc agcgtatcga ccgtgtcgat caccggacgg atttcccggg cgcgctccag
     2341 ttccgacgat ttgcgcgggc cgtccagcac ggcggggtcc cacacccggt tcaggtcctc
     2401 atcgacgaag cgcgacagcg tcgggtgccg cggatcaaag cgggcgaagg cggcgaggtt
     2461 gacgaagccg aacgtcagcc ggccgcgccg tggagccagc ccgctgcgca gcagccggtc
     2521 cagcacgatg ccgccggcga tctcgttgcc atgcgacagg cccaggatcg ccacgtgcgg
     2581 gccgggcttg ccggaatcgc gcgtggtgaa cccgggaacg ccgatattgc ccgggatcca
     2641 gtcgccgatc tgcggcgcgg tgagccgcac gtcgaaatgg ggcaggggtg gcgggatcgg
     2701 ctggcccatc gaacgtctcc cacgatcagg tcagcaggcg gtccgccagc cggcgtatga
     2761 aggcgtcgca tgcgtccaac tggtcctgcg ccagccattc gtccggctgg tgtgcctgcg
     2821 cgatatgccc cgggccgcag atgatcgtgg caattccggc attctggtaa aagccccctt
     2881 cggtgccgta actcaccttg cccgtatcgt tcgacccggt cagttgcttg acgattgcgg
     2941 tcaattcatg gtcggcgcca agcgccagcc cgggcatgcg caccggcact tcgtagctga
     3001 acccgcaatt cggattcacc gcacgcatcg ccggctcgat atggttggcg atatgcacct
     3061 gcatgcgctc cagcgccgcc tcggcgtcat cgcctggaat gttgcgccat tccatcatga
     3121 actcggcatg ctcgggaatg atgttcagga tgctgccgcc ctgcacggtg ccgacatgga
     3181 tggtggtgtg cggcggatcg aacccgtcct cgaacggccc ttccttctcc agccgccgcg
     3241 cttcggcggc gacccaggcg atcgcctcgg cggcggcctg taccgcgttc acccctttcg
     3301 ccggcacgct ggaatggccg ggcaggccgc gcaccttcac ccgcacgtcc agcttcccct
     3361 tatgcgccag gatcggcttc atgccggacg gctcgcccac cacgcacagc gccggcttca
     3421 ggccggattc gtccaggtcc tcgatcagcc ggatggcgcc ctggaaatcg acttcctcgt
     3481 tgtagctgat gaacagatgc agcggcttca ccggcttgcg cgccaggaag tccggcaccg
     3541 cggcgaggca ggacgcgaca aaccccttca tgtcgcaggc gccgcgcgcg tacagcttgc
     3601 cgtccgcctg gcgcagggtg aagggatcgg cgctccacgc ctgaccgtcc accggcacgg
     3661 tatcgacatg cccggacagc gcgaccccgc cggcggtctg cggaccgatg atcgcgtgga
     3721 tgttggcttt ctgcccggcg ggatcggtgc tgatccggta cggcaccccg tgcgcgtcaa
     3781 gatagccgcg cacgaacgcg atcagcttca ggttggaatt gcggctggtg gtatcgaacg
     3841 cgacgagacg ggccaggagt tcggcggcgc tcatgcgggg ggcggaattg ggagcattca
     3901 tgccgccact ctataccgac cggcggcagc ggcaaacgca ccggcatcag ccttcagatt
     3961 ccggcgtgcc gtccggagca accgcctggc ggggtgccgg gggttgctgt tcctgccgga
     4021 tgggccgcgc gatcgggtgg tgtgacgcga cgaccggcgc gcgtggcgag cattgcctcg
     4081 gcgggctgtt tcctgcatgt actggccggt aaggaccgag gtcgcccatg cccttcctga
     4141 ccgaaccgac gcccgagcgc gggatcatcc tgccggtgct gcccggcatc agccgcatcg
     4201 tcgccgacaa tccggggccg atgacctatt acggcaccaa cacctatctg atcgagacgg
     4261 atgacggcat ggtggtgctg gatcccggtc cggaagacca tcccgagcac gtcgaggcga
     4321 tcatgcgtgc caccggcggg gcggtggcgc tgatcctggt cagccacacg catcacgacc
     4381 atgtcggcgc agtgcccgcc ttgcaggatg cgaccggcgc gccgacggtc gggttccagg
     4441 aaagcatgct cgacagcttc gacgccgata tcaaactggc ggacggcgat accatcgcgg
     4501 ggatgcaggc actgcacacg cctggccatg cctccgacca tctgtgtttc tcgcttgcgg
     4561 cgtcggacgg aacagcggtg ctgttcagcg ccgaccacgt gatgtcgtgg tccaccagcg
     4621 tggtcagccc gccgggcggc gacatggcgg cgtatttctc cagcctgcgc ctgctgctgg
     4681 accgcaccga cgatgtgtac ctgcccggcc acgggccgcc cctgccggag ccgcgcgaat
     4741 tggtgcgcga gatgctgcat caccgcatgg cgcgcgaacg ggccatcgcc accaggctgc
     4801 aggaaggccc cgccgacacc ttcgtgctga tggacacgct gtattcgcag gtgcacccca
     4861 ggctgcgccg tgcggcggag cgcaacgtgc tggcccatct gctgaagatg gaactggagg
     4921 gcaaggtggt gcgcgacgga gacttgtggc gcgccgcctg atcaggattg ccagttcgtc
     4981 ccgcggatcg ccaaggaggc tcctgtcccg atgacccagc cgcagatcga ctccgcccgc
     5041 ctgctcgccg acctgcacgc cctgcgcggc atcggcagct acaagaccgg cgcccaccgc
     5101 ccgacctact cgccgcagga catgcagtcc cgccactggc ttgccgatcg catgacgcag
     5161 gccgggctga cgccggagat cgacggcgtc ggcaatgtca tcggccgcgc ggccggtccc
     5221 ggcccgcacc tgctgctcgg ctcgcacatc gagacccagc cgcatgccgg ttggctggac
     5281 ggcgcgctgg gcgtgatcta cgggctggag gtcgcccgtg cgctgggccg caacatcgat
     5341 gtggctgcct gggcggatga agagggccat ttcgacaatt tcgtcggcag ccgttccttc
     5401 accggcaacc tgaccgaggc cgatatcgac cgcgcccgca accgctatga cggcacgccg
     5461 ctgcggaacg cgctgcaagc cgccggcttg gccggacgtc cccgcgcgca ggtcgatccc
     5521 gcccgttacc gcggctatct ggaggcgcat atcgagcaag gcgacgaact cgacaccagc
     5581 ggcaagcggc tgggcgtcgt caccagcatc gtcgccatct accagtaccg catccgcttc
     5641 cacggcgtgc agaaccacgc cggtaccacg cgcatggcga tccgcaagga tgccgggctg
     5701 gcgctggtcc ggctcgccgc cgcgatcgac cgccgcttcc cggaagtggc cggtccgcgc
     5761 tcggtctgga ccaccggccg catcgcgctg gaaccgggcg cggccagcat cgtgcccggc
     5821 ggggccgaga tgatgttcca gttccgcgat gccgatccgc agcgtctcgc ggtgctgcac
     5881 cagacgctgg agcaactggt cgccgaggcg aatgccggcc cctgccgggt ggagttgcag
     5941 gtgatgtcgc agtccacgcc gaaggtgatg gatgccggct tccaggacgc gctcgccgcg
     6001 gcggcggaag cgcgcgcgcc gggaaactgg gttcgcatgc ccagtggcgc cgggcacgac
     6061 gcacaggtgc tggcccaggc gatgccggcc ggcatgctgt tcgtgccgag cattggcggc
     6121 atcagccatc attggtcgga agacaccaag gaggaggata tcgtccttgg ctgccaggtg
     6181 ctgaccgatg ctgccgccgc actgcttgcc tgaccggcgc ggggtcgtgg ggggactata
     6241 ccgcccc
//
LOCUS       DASSZH010000554         4081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_422081, whole genome shotgun sequence.
ACCESSION   DASSZH010000554 DASSZH010000000
VERSION     DASSZH010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422081"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..998
                     /locus_tag="VFN42_12065"
     CDS             225..998
                     /locus_tag="VFN42_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HET8997392.1"
                     /translation="MSELPTLLAPGEPDPVRLLNPEGQSAFVLIADHAGREIPRSLAR
                     LGVPESELLRHIAWDIGIAGVTERLADTLDAVAVLQRYSRLVIDCNRDPASPTSIPEI
                     SEHTPIPGNTGLKPAQRQARVDAVFRPYHDHIARLLDARAASGRRTVLIAMHSFTPVF
                     KGQTRGMHVGVLYHHEQRLAHIMLELLRAEGDLVVGDNAPYAITDTSDYAVPVHGEAR
                     GLPLVELEIRQDLIAEPGGQAAWAARLARLLPEAAKRLG"
     gene            complement(1002..2552)
                     /locus_tag="VFN42_12070"
     CDS             complement(1002..2552)
                     /locus_tag="VFN42_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HET8997393.1"
                     /translation="MATAHAATLAEHTPHRGMITACAIGATLLQSLDQTIANVALPYM
                     QGSFSASYDEITWVLTSYIVMAAIMTGPVGWLASRFGRKPLFVICIVGFTVASMACGA
                     AQSLGQIVTFRLIQGAFSAALVPLSQAILLDIYPHEQRGFAMAVWGLGVMIGPILGPT
                     LGGYLTDFYSWRYVFYINLPFGVLATAGLVIFLPHIEGQDTLRFDWLGFSVLAIGIGA
                     LQLMLDRGQNQDWFSSREIIIEAVLAGLGLYLFLVHVLYAPRPLIRPAMFRDLNFASG
                     LVMMFAVGTILVSSIALMAPWLQTLAEYPVAAAGLIMAPRGLGNVTTILISGRMSNRM
                     DPRLLVGSGLLLECWSFWEMTSWTPDVSVQHIIATIFIQGAGLGLVFTPLQVLAFATL
                     APSLRTEGASLFSLARNVGAAIGVSVTTSLLAHNAQILHEQIGASVTPFNRALQVGGA
                     IRHFLSPETAHGAALLNRMIDHQAQIIGYDNDYVLMTMTTLPALLLLLFMRRPRSAAA
                     VAEMQPAD"
     gene            2784..3752
                     /gene="fabD"
                     /locus_tag="VFN42_12075"
     CDS             2784..3752
                     /gene="fabD"
                     /locus_tag="VFN42_12075"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET8997394.1"
                     /translation="MPVHAFIFPGQGSQAVGMGRELAAAFSAAREVFQEVDETLHQKL
                     SQLMFEGPAEELTLTENTQPALMAHSIAVLRVLERDGGIALKQKAVVVAGHSLGEYSA
                     LAAAGAFSLPDAARLLKLRGQAMQKAVPAGQGAMAALLGADMSQAQAICEQAAPVPGS
                     ETHERQVVQPANDNGAGQVVISGHREAVERAIPIATAMGIRRAMLLPVSAPFHCALMA
                     PAADAMAEAFGTTPPGAPLVPLIANVSAAKATDPEEIRDLLVRQVTATVRWRECVEAM
                     VALGVDSFVELGAGKVLSGLMRRIAPDAKAAAAATPADIEALLKNL"
     gene            3767..>4081
                     /locus_tag="VFN42_12080"
     CDS             3767..>4081
                     /locus_tag="VFN42_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8997395.1"
                     /translation="MFRLDGKAALITGASGGIGAAIARMLHAQGAAVVLSGTRQDTLN
                     ALAGELGERAHVCPADLADAAAAEALVTAAEEAAGPLHVLVNNAGFTRDMLALRMSDE
                     DWG"
BASE COUNT          687 a         1367 c         1433 g          594 t
ORIGIN      
        1 gccagttgca gcagcgcaat accgtctcca ccccgcggaa cggaacgggc caggtcaagt
       61 cgggggaggt ctcgggccat gcacgcatcc actctgcgcc cgcggccata ggacgtgccc
      121 cggactccaa tgccagccgg ctccgctata cccgcgaacc ccgcacctgg acattgggcc
      181 acgggtgcgg cagtcccagc caggagccgt cacgcggagg agccatgtcg gaactgccaa
      241 ccctgctcgc gcccggcgag ccggatccgg ttcgcctgct gaacccggag ggacaatccg
      301 cgttcgtgct catcgccgac catgccggcc gcgaaattcc gcgttccctg gcccggcttg
      361 gcgtcccgga gagcgagctg ctgcggcata tcgcatggga tatcggcatc gccggcgtca
      421 ccgagcgcct ggcggacacg ctggatgcgg tcgccgtact gcagcgctat tcgcgcctgg
      481 tgatcgactg caaccgcgat cccgcctcgc cgacatcgat cccggagatc agtgagcaca
      541 cgccgatccc cggcaacacc gggctgaaac cggcgcaacg ccaggcccgg gtggatgcgg
      601 tgttccggcc gtaccacgac cacatcgcgc gcctgttgga tgcacgcgcc gcctccggcc
      661 ggcgcaccgt gctgatcgcc atgcacagct tcaccccggt gttcaaaggc caaacgcgcg
      721 gtatgcacgt gggcgtgctg taccatcacg agcagcggct ggcgcatatc atgctggagc
      781 tgttgcgggc ggagggcgat ctggtggtag gcgacaacgc gccctatgcc atcaccgata
      841 ccagcgacta tgccgtgccg gtgcatgggg aagcgcgcgg tttgccgctt gtggagctgg
      901 agatccgcca ggacctgatc gcggagcccg gcggccaggc ggcctgggcc gcaaggctgg
      961 cgcggctgtt gccggaagcc gccaagcggc tcggctgatc ctcaatccgc cggctgcatc
     1021 tccgccaccg ctgccgcaga gcgcgggcgg cgcatgaaca gcagcagcag cagcgcgggc
     1081 agggtggtca tcgtcatcag aacatagtcg ttgtcgtagc cgatgatctg cgcctggtga
     1141 tcgatcatgc ggttcagcag cgcggcgccg tgcgccgtct ccggcgacag gaaatgccgg
     1201 atcgcgccac ccacctgcag cgcgcgattg aacggggtga ccgatgcgcc gatctgttcg
     1261 tgcaggatct gcgcgttgtg cgccagcagc gaggtggtta ccgacacgcc tatcgccgcg
     1321 ccgacattgc gtgccaggct gaacagcgac gcgccctcgg tgcgcagcga cggcgccagg
     1381 gtggcgaagg ccagcacctg caacggcgtg aacaccagcc ccagtccagc gccctggatg
     1441 aagatggtgg cgatgatgtg ctgcacggaa acgtccggcg tccagctcgt catttcccag
     1501 aacgaccagc attccagcaa cagtccgctg ccgaccagca ggcgcgggtc catccggttg
     1561 gacatgcggc cgctgatcag gatggtggtc acgttaccca gcccgcgcgg cgccatgatc
     1621 aggccagccg ccgccaccgg atactctgcc agcgtctgaa gccagggcgc catcagcgcg
     1681 atgctggaca ccaggatggt gccgaccgcg aacatcatca ccaggccgga ggcgaagttc
     1741 aggtcgcgga acatcgccgg ccgtatcagc gggcgcggcg cgtacagcac gtgcaccagg
     1801 aagagataca gccccagtcc ggccagcacc gcctcgatga tgatctcgcg ggagctgaac
     1861 cagtcctggt tctggccgcg gtccagcatg agctgcagcg cgccgatccc gatcgccagc
     1921 acgctgaacc ccagccagtc gaagcgcagc gtgtcctgac cctcgatatg cggcaggaag
     1981 atcaccagcc cggcggtggc cagcacgccg aacggcaggt tgatgtagaa gacgtagcgc
     2041 cagctataga agtcggtgag gtagccgccg agcgtcgggc ccaggatcgg cccgatcatc
     2101 acgcccagcc cccagaccgc catggcgaaa ccgcgctgct cgtgcgggta gatatccagc
     2161 aggatcgcct gcgacagcgg caccagtgcg gcgctgaagg cgccctggat cagccggaac
     2221 gtgacgatct ggcccagcga ctgcgccgcc ccgcaggcca tggaggcaac ggtgaagccg
     2281 acgatgcaga tgacgaacag cggcttgcgc ccgaaccggc tggccagcca gccgaccggc
     2341 ccggtcatga tcgccgccat cacgatgtag gaggtcagga cccaggtgat ctcgtcgtag
     2401 ctcgccgaga aactgccctg catgtaaggc agcgcgacat tggcgatggt ctggtcgagc
     2461 gactgcagca gcgtcgcccc gatggcgcag gcggtgatca tgccgcgatg cggggtgtgc
     2521 tccgcaagcg tggcagcatg ggcggtggcc atggcctaac gactggccga cggcatgcgg
     2581 gacattcctc cgggcggcgc ttcgcggtgg ggccgcttga gcgggcggac tgtggtgcat
     2641 gtcgccggtg cgatcaagca caggcagggc tgttcacgtg gttccaacac ggaaccggga
     2701 aggcgcgctg cattgacagt ctcgtgaacc cagggctacc ccgcctccct gttacagaat
     2761 ggccatctcg ggagccgagt cgcatgccgg tccacgcctt catctttccc ggccagggca
     2821 gccaggctgt cggcatgggg cgcgagctcg ccgccgcctt ctccgccgcg cgggaagtgt
     2881 tccaggaggt ggacgagacg ctgcaccaga agctgtcgca gctgatgttc gaaggcccgg
     2941 ccgaggaact gacgctgacc gagaataccc agccggcgtt gatggcccac tcgatcgccg
     3001 tgctgcgggt gctggagcgg gatggcggca tcgccttgaa gcagaaggcg gtggtggttg
     3061 ccggccactc gctgggcgaa tacagcgcgc tcgccgccgc cggggccttt tccctgcccg
     3121 atgccgcccg gctgctgaag ctgcgcggcc aggcgatgca gaaggcggtg ccggccgggc
     3181 agggcgccat ggcggcgctg cttggtgccg atatgtcaca ggcgcaggcg atctgcgagc
     3241 aagccgcgcc ggtgccgggc agcgaaacgc acgagcgcca ggtggtgcag ccggccaatg
     3301 acaatggcgc cggccaggtg gtgatctccg gccatcgcga ggcggtggaa cgcgccatcc
     3361 cgatcgccac tgccatgggc atccggcgcg ccatgctgct gccggtctcc gctccattcc
     3421 actgcgcgct gatggcgccg gccgccgatg cgatggccga ggcgtttggc acgacgccgc
     3481 cgggcgcgcc gctggtgcca ctgatcgcga acgtctcggc cgccaaggcg accgacccgg
     3541 aggaaatccg cgacctgctg gtgcgccagg tcaccgccac ggtgcgctgg cgtgagtgcg
     3601 tggaggccat ggtggcgctc ggggtggaca gcttcgttga actcggcgcc ggcaaggtgc
     3661 tgtccggact gatgcggcgc atcgccccgg atgcgaaagc cgccgcggcc gccacaccgg
     3721 ccgatatcga ggcgttgctg aagaacctct gacggaggga cggaccatgt tccggctgga
     3781 tggcaaggcg gcgctgatca ccggcgcttc gggcggcatc ggcgcggcga ttgcgcggat
     3841 gctgcatgcg cagggcgcgg cggtggtgct gtccggcacg cggcaggata cgctgaacgc
     3901 gctcgccggg gagctggggg agcgggcaca tgtctgcccg gccgatctgg ccgatgcggc
     3961 tgctgccgag gcgctggtga ccgcggccga ggaagcggcc ggcccgctgc acgtgctggt
     4021 gaacaacgcc ggtttcaccc gcgacatgct ggcactccgc atgagcgacg aggattgggg
     4081 c
//
LOCUS       DASSZH010000555         3954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_644009, whole genome shotgun sequence.
ACCESSION   DASSZH010000555 DASSZH010000000
VERSION     DASSZH010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644009"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VFN42_12085"
     CDS             complement(<1..852)
                     /locus_tag="VFN42_12085"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HET8997396.1"
                     /translation="MLFHSQPFILGFLPLCLAGFFLLGRHAGAVWALRWLLLASLFFY
                     GWWKPKFVLLLAGSILVNDAIARRIRATAPGPAARCWLIAGIACNLVLLGWFKYADFL
                     LHALAPHAPSLQVFLPLAISFFTFQQIMFLVGSFRGECTGCGFLQYASFIAFFPHLIA
                     GPIVRPRDIIPQLSARCFAIPCMDNLAAGLLFFLLGLAKKLVLGDTFGGFADIGFNAA
                     AHGASLSLLEAWYAVLAYALQIYFDFSGYSDMAIGVARMLNVEFPANFASPYQARNIG
                     EFWRRWHI"
     gene            complement(943..2034)
                     /locus_tag="VFN42_12090"
     CDS             complement(943..2034)
                     /locus_tag="VFN42_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997397.1"
                     /translation="MDPRRYLAVLSITAVVAFAIVWVWVAAMPLAFLDPEYPAWRAKL
                     DLLHRCDLGTVLIVGDSRAAVDIIPALLPIRASNLAVGGGETIEAYVTLSRALACKAP
                     PRRVVISLDAAHFTHPDLFWERSVRFGLVGLADLADLRRVETQTGDSSFAAPQRTDGM
                     PSRFRAWAYAWRFPAVYFNSLLRGGVLLRWWENRAAYAAALASRGQYFFGQDAGSSAV
                     AAEGHLRAFQPLPVLDRYFDRTLALLAQRGIPADFIAMPMNRSTHAAVRPSVEAEFVR
                     YLAGYAARYPNFHLVGAAMPDWPNRYFGDAFSHLNRTGAQRLSTALGECLLRRMAAGR
                     AGACTLANAWMATAENRRIRADILAGEQD"
     gene            complement(2034..3569)
                     /locus_tag="VFN42_12095"
     CDS             complement(2034..3569)
                     /locus_tag="VFN42_12095"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HET8997398.1"
                     /translation="MLFNSYLFVAGFLPPVFGGFWIACRIGPRAAAAWLLTASLLFYG
                     WWNPAFLVLLLASIAGNYALGALILRTGNRQRLHSAILIFGIGANLGALAYYKYLGGV
                     LAFLRLHSVAGPGFATPILPLGISFFTFTQLGYLLDCWQGQVKRDPPLLHYALFVSFF
                     PHLIAGPILHHRELLPQFAERQAWRVDAQNVAVGSAIFLIGLLKKTLLADPISAPVAP
                     GFVHPELLTLFPAWHVALSYSLQLYFDFSGYSDMAIGLARMFNLRFPVNFNAPYRSES
                     VIDYWQRWHMTLTRWLMLYLYNPLALHLARRRAARGLPTGMEARRTLRGFAGMIAAPT
                     LVTIGLAGIWHGSALTFVVFGLLHAAYLCVNHAWRLWHVGQPSTAPAARLGRVLLTYG
                     CVLVGSVVFRAPDLSAAAQVLAGMFGVHGLMPLLPDAKAIARAGLDVLWLAGLYAIVW
                     AAPTTQQIMRAHGPGPGRVTAGPLPRLLWQPTLGWAVAMGCGAALALLSIGGTGEFLY
                     FQF"
     gene            complement(3577..3846)
                     /locus_tag="VFN42_12100"
     CDS             complement(3577..3846)
                     /locus_tag="VFN42_12100"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET8997399.1"
                     /translation="MPGCQAADGPVLAQIADIVRDLLRDPELEVSEQSTPDDLPGWDS
                     MTHIALIVESECRFGVQFRTGEIEDLHSVGDLVRSIRLKQIRAAT"
BASE COUNT          822 a         1279 c         1322 g          531 t
ORIGIN      
        1 gatgtgccag cggcgccaga attcgccgat attgcgtgcc tggtatgggc tggcgaagtt
       61 ggccggaaat tccacgttga gcatgcgcgc cacgccgatc gccatgtcgg agtagcccga
      121 gaagtcgaag tagatctgca gcgcgtacgc caggacggcg taccaggcct ccagcagact
      181 cagcgaggcg ccgtgcgccg cggcattgaa gccgatatcg gcgaagccgc cgaacgtgtc
      241 gcccagcacc agcttcttcg ccaagccgag caggaagaac agcaggcccg cggccaggtt
      301 atccatgcaa gggatggcga aacagcgggc ggaaagttgc gggatgatgt cacgcgggcg
      361 cacgatgggt ccggcgatca ggtgcgggaa gaatgcaatg aagctggcat attgcaggaa
      421 gccgcagccg gtgcattcgc cacggaagct gccgaccagg aacatgattt gctggaaggt
      481 gaagaagctg atcgccagcg gcaggaacac ctgcaaagac ggtgcgtgcg gtgccagtgc
      541 gtgcagcagg aaatcggcgt atttgaacca acctagcagg accaggttgc aggcgatccc
      601 ggcgatcagc cagcatcggg ctgcagggcc cggcgcggtg gcgcggatgc gacgggcgat
      661 ggcgtcattg accaggatcg agcccgccaa cagcaacacg aattttggct tccaccagcc
      721 gtagaagaac aggctggcca ggagcaacca gcgcaacgcc cacaccgccc cggcgtggcg
      781 cccgagcaga aagaagcccg ccaggcacag cggcaggaag ccaagaatga aaggctgaga
      841 atggaacaac acgggtggcg agtgtgccgc ggattgcata agcaaaggta aacgcccggc
      901 acgtcgcatg gacctgctgc cggtggagcg cttcgcgccg gttcagtctt gctcgcctgc
      961 gaggatatcg gcgcggattc ggcgattttc tgccgtcgcc atccaggcat tcgccagggt
     1021 gcacgcacca gcgcgacccg ccgccatccg tcgcagcagg cactccccca gcgcggtgct
     1081 cagccgttgt gcgccagtcc ggttcaagtg agagaacgca tcgccgaaat agcggttcgg
     1141 ccagtccggc attgccgcgc cgaccaaatg gaaattcggg tagcgtgcgg catagccggc
     1201 caggtaacgc acgaactccg cctcgacgct tggacgcacc gcggcgtgcg tgctgcggtt
     1261 catcggcatg gcgatgaaat cggccggaat cccccgctgc gccagcaatg ccaatgtgcg
     1321 gtcgaaatag cggtcgagca ccgggagcgg ctggaatgcc cgcagatgtc cctccgccgc
     1381 gacggccgag gatcccgcgt cctggccaaa gaaatactgc ccgcgcgatg ccagcgccgc
     1441 tgcataggcc gcccgattct cccaccagcg tagcagcacg ccaccgcgca gcaggctgtt
     1501 gaaatacacg gccgggaagc gccaggcata tgcccaggcc cggaaccgcg acggcatgcc
     1561 gtcggtgcgt tgcggcgccg cgaagctgct gtcgccggtt tgcgtctcca cccgccgcag
     1621 gtcggccaga tccgccaggc cgaccagacc gaagcgcacc gagcgctccc agaacaggtc
     1681 ggggtgggtg aagtgcgcag cgtccagcga tatcaccacc cggcgtggcg gcgccttgca
     1741 tgccagggcg cgggacaggg tcacataggc ctcgatcgtc tcgcccccgc ccacagcgag
     1801 gttgctggcg cggatcggca gcagcgccgg gatgatatcc acggcggccc gggaatcgcc
     1861 cacgatcagc accgtaccga gatcgcagcg gtgcagcagg tccagcttgg cacgccatgc
     1921 cggatattcc ggatcgagga aggccagcgg cattgccgcc acccagaccc agacgatggc
     1981 gaacgccaca accgcggtga tggacagcac ggcaaggtag cgccgggggt ccatcagaac
     2041 tggaagtaga ggaattcgcc ggtgccgccg atcgacagca acgcaagtgc ggcgccgcag
     2101 cccatcgcca ccgcccaccc gagcgtcggc tgccacagca atcgcggcag tggtcccgcg
     2161 gtgacgcgac ccggccccgg accatgcgcg cgcatgatct gctgcgtggt cggcgccgcc
     2221 cagacgatcg cgtacagacc ggccagccac agcacatcca acccggcccg cgcgatcgcc
     2281 ttggcgtcgg gcaggagcgg catcagcccg tgcaccccaa acatgccggc gagcacctgt
     2341 gccgcggcag aaaggtccgg cgcacggaac accaccgaac cgaccagcac acacccatag
     2401 gtcaatagca cacgcccgag ccgcgccgcc ggtgccgtcg aaggttgccc gacatgccac
     2461 agccgccagg catggttcac gcacaggtag gccgcatgca gcaggccgaa cacgacgaag
     2521 gtgagcgcgc tgccatgcca gatgcctgcc agtccgatgg tgaccagcgt cggcgcggcg
     2581 atcatgccgg cgaagccgcg cagcgtgcgc cgggcctcca tccctgtcgg caggccgcga
     2641 gccgcccggc ggcgcgcaag gtgcaatgcg agtgggttgt agagatacag catcaaccag
     2701 cgggtcagcg tcatgtgcca gcgttgccag tagtcgatga cgctctccga ccggtagggc
     2761 gcgttgaagt tgaccgggaa gcgcaggttg aacatgcggg ccaggccgat cgccatgtcg
     2821 gaatagccgg aaaagtcgaa atagagctgc agcgaatagg acaatgcgac gtgccacgcc
     2881 gggaacagcg tcagcagttc cggatgcacg aaaccgggcg cgaccggcgc ggagatggga
     2941 tcggccagca gcgtcttctt gagcagccca atcaggaaga tcgcgctgcc gactgccacg
     3001 ttctgcgcgt caacccgcca ggcctgccgc tcggcgaatt gcggcagcaa ttcccgatgg
     3061 tgcaggatcg gcccggcgat cagatgcggg aagaagctga cgaacagcgc gtaatgcagc
     3121 agcggcgggt cacgcttcac ctgcccctgc cagcaatcca gcaaatagcc aagctgggtg
     3181 aaggtgaaga agctgatgcc aagcggcagg atgggggtgg cgaagccggg gcctgcgacg
     3241 ctgtgcagac gcaggaatgc aagtacccca ccaagatact tgtagtaggc caaggctccg
     3301 agattggcgc cgatgccgaa tatcagaatg gcgctgtgca gccgttgccg gttgccggtg
     3361 cgcaggatca gcgcgcccaa agcgtaattg ccggcgatgg aggccagcag caggaccaga
     3421 aaggccggat tccaccagcc gtagaacagc agcgatgcgg tcagcagcca cgccgccgcg
     3481 gcgcgcgggc cgatccggca agcgatccag aacccgccga acaccggcgg cagaaagccg
     3541 gcgacgaaca gataggaatt gaacagcacg gctcggtcac gtcgcggccc gaatctgctt
     3601 caggcggatg gagcgcacca ggtcgccgac actatgcaga tcctcgattt cccccgtgcg
     3661 gaactgcacg ccaaagcggc actcggattc cacgatcaac gcgatatgcg tcatggaatc
     3721 ccagccgggc aggtcgtccg gcgtgctttg ctcggacact tccagctccg gatcgcgcag
     3781 caggtcgcgg acaatgtcgg cgatctgggc gagcaccgga ccgtcggcag cctggcatcc
     3841 cggcatggca ttcctccggc atcgtcgtga tggcgggcat tacacgccac agcgattacc
     3901 gaagggttaa tctccgggcg ggagtcttgc ggccgctaac cggtggctgc cacg
//
LOCUS       DASSZH010000556         2415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1206515, whole genome shotgun sequence.
ACCESSION   DASSZH010000556 DASSZH010000000
VERSION     DASSZH010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206515"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VFN42_12105"
     CDS             complement(<1..255)
                     /locus_tag="VFN42_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET8997400.1"
                     /translation="MNISAPFIARPIATWLLAIAILLAGALGYRSLPVSALPEVDFPT
                     IQVTTQLPGASPETVETLITASLERQFGQIQGLKLMTSESS"
     gene            complement(259..1503)
                     /locus_tag="VFN42_12110"
     CDS             complement(259..1503)
                     /locus_tag="VFN42_12110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET8997401.1"
                     /translation="MDALTRDTTELQARQRTRRWPMVLACLAVLGAIVAVIWLWHPGA
                     SKKAAHTRPEQAVPVVDAAVTQRNVPIYLDGLGTVQAFNTVTIKPMVDGPLIAVDFKE
                     GQDVRKGDVLAQIDPRTYQAALDQALAKKAADQALLANARLDYARYQKLVANKYTSAQ
                     QADTARAQVAQDQAQIQQDDALIETARTNLGYTTIRSPIDGRTGIRQVDAGNIVHASD
                     ATGLVVITQLHPISVIFTLPQQSLGAVTAAMRKGTPTVLAYAEGANGSPANVLDTGTL
                     SVLDNQVDPTTGTIKLKATFPNPGDRLWPGAFVGVKLLARTAENAVVVPPAAVQRGPQ
                     GTYVFQVTGSMVKLQPVTVGHEDAQQSIITQGLKPGDQVVVDGASRLSNGSKIKIVAP
                     APANPAAGPEPTSAPGTRPHAG"
     gene            complement(1705..>2415)
                     /locus_tag="VFN42_12115"
     CDS             complement(1705..>2415)
                     /locus_tag="VFN42_12115"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET8997402.1"
                     /translation="TVRPGTLTALTLPPVAPGLPSQLLERRPDIAAAEAQLEAAEFDI
                     KVARAAFFPSIQLTGSAGFQNAALSTLMGPGGFLASLAAGLAQPLFDGGTLRGQLEQA
                     KGRQQELLANYHKAVVQAFTDVDNALTAWRYTSEQEELQRRAVATAQRAADIARAQLQ
                     AGTVDITTVLQAETTLFGDEDTLVQVRLARVQALVDLYKALGGGWVQPAGSPRQQFPG
                     LSPGLVPGGVALPIGGNR"
BASE COUNT          333 a          848 c          817 g          417 t
ORIGIN      
        1 gctgctctcc gaggtcatca acttcagccc ctggatctgg ccgaactggc gctccaggga
       61 ggcggtgatc agcgtctcca ccgtttccgg actggcaccg ggaagctggg tggtgacctg
      121 gatggtcgga aaatccacct ccggcagggc ggagaccggc agcgagcggt agcccagcgc
      181 gccggccagc aggatggcga tcgccagcag ccaggtggcg atcgggcggg cgatgaaggg
      241 ggcggagatg ttcatcggtc agccggcgtg tggccgggtc ccaggcgcgc tggtgggctc
      301 gggtcctgcc gccgggttgg cgggggccgg agccacgatc ttgatcttgc tgccgttcga
      361 cagccgcgac gcgccgtcca ccaccacctg gtcgccgggc ttcaggccct gggtgatgat
      421 cgactgctgt gcgtcctcat gtcccacggt gaccggctgc agcttcacca tgctgccggt
      481 gacctgaaac acgtaagtgc cctgtggccc gcgctgcacc gcggccggcg gcaccaccac
      541 ggcgttctcc gcggtgcgcg ccagcagctt caccccgacg aatgcgccgg gccatagcct
      601 gtcgcctggg ttggggaagg tggccttcag cttgatcgtg cctgtggttg ggtcgacctg
      661 gttgtccagc acgctcagcg ttccggtatc cagcacgttg gccgggctgc cattggcgcc
      721 ctcggcgtag gccagcaccg tcggcgtgcc cttccgcatc gccgcggtca ctgcgccgag
      781 cgactgctgc ggcagggtga agatgaccga gatcgggtgc aactgggtga tcaccaccag
      841 cccggtggcg tccgaggcat gcacgatatt gccggcatcc acctggcgga tgccggtgcg
      901 gccgtcgatc ggcgagcgga tcgtggtgta accaaggttg gtgcgcgcgg tttcgatcag
      961 cgcgtcatcc tgctggatct gcgcctggtc ctgtgccacc tgcgccctgg cggtgtcggc
     1021 ctgctgcgcg gaggtgtatt tgttcgccac cagcttctgg tagcgcgcgt aatccagcct
     1081 ggcgttggcc agcagcgcct ggtcggcggc cttcttcgcc agtgcctggt ccaaggccgc
     1141 ctggtaggtg cgtggatcga tctgcgccag aacgtcgccc ttcctgacgt cctggccttc
     1201 cttgaaatcc accgcgatca gcgggccatc gaccatcggc ttgatcgtga cggtattgaa
     1261 cgcctggacg gtgccaagcc cgtccagata gatcggcaca ttcctctgcg tcaccgccgc
     1321 gtccaccacc ggcaccgcct gctcgggccg cgtgtgcgcg gccttcttgg aggcgcctgg
     1381 gtgccacagc cagatcaccg ccacgatggc gcccagcacc gccaggcagg ccagcaccat
     1441 gggccagcgc ctggtgcgtt ggcgggcctg caattcggtg gtgtctcggg tcagcgcgtc
     1501 catgcgggtt ctcgattcat cggatcgcga tatcgggatg cgggcaggca acgccaatgt
     1561 cgtgctgtgg gttgttggcc gcgccctggc cggacctgcc ctgccgtctt cccggactga
     1621 acgggacacg atgtaatggt gcatgcgcgc tcgcgggcat gacacagggg acggccgtcc
     1681 tcggccaagg catgcttgtc gccatcagcg attccccccg atcggcagcg ccacgccgcc
     1741 cggtaccagc cccggcgaca agccggggaa ctgctgccgc ggcgatcccg ccggctgcac
     1801 ccagccgcca cccagcgcct tgtacagatc caccagcgcc tgcacccgag ccagccgcac
     1861 ctgcaccagc gtgtcctcgt cgccgaacag cgtggtttcc gcctgcagca ccgtggtgat
     1921 gtccaccgtg ccggcctgca gctgcgcccg ggcgatgtcc gcagcccgct gcgctgtcgc
     1981 caccgcgcgc cgctgcagct cctcctgctc ggaggtgtag cgccacgcgg tcagcgcgtt
     2041 gtccacgtcg gtgaacgcct gcaccaccgc tttgtggtaa ttcgccagca gttcctgctg
     2101 gcgtcctttc gcctgctcca actggccgcg cagcgtgccg ccgtcgaaca gcggctgcgc
     2161 cagcccggct gcaagggagg ccaggaatcc cccgggcccc atcagcgtgg acagcgccgc
     2221 gttctggaat ccggccgatc cggtcagctg gatgctgggg aaaaacgccg cccgcgccac
     2281 ctttatgtcg aactcggccg cctccagctg cgcttccgcc gcggcgatat cgggccgccg
     2341 ctccagcagc tgcgatggca atcccggcgc caccggcggc agcgtcagcg cggtgagcgt
     2401 gccgggacgc acggt
//
LOCUS       DASSZH010000557         2511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_422097, whole genome shotgun sequence.
ACCESSION   DASSZH010000557 DASSZH010000000
VERSION     DASSZH010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422097"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="VFN42_12120"
     CDS             complement(<1..876)
                     /locus_tag="VFN42_12120"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET8997403.1"
                     /translation="MTPIDFIVLTGFLGSGKTTLLRDFLALPEAADTGVIVNEVGQMG
                     LDGALLAERGDSPVALLSNGCVCCSLGSDLERTIAGMLAVRQDAGLAPPSRMILETSG
                     LAKPGPILRSMASLGSLGLRVGVVATFDCTRGATLGNFEEAAAQWAGAQTLVVTKRDL
                     ASPAQLRDAPRLAAGINPTAELIDTDDRRAAALGALSLRAPPSLRGLLPPAEAVGLQH
                     PRIGVVLARLDAVVAWEALAEWLDNLAGRCGERLLRIKGLVQVTGSSRPLLVQSVGTV
                     FSAPRAFAGEAGNGSF"
     gene            complement(873..>2511)
                     /locus_tag="VFN42_12125"
     CDS             complement(873..>2511)
                     /locus_tag="VFN42_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8997404.1"
                     /translation="WPDPRSLTMADDTFEPDAGEELTAQRAQQGMPALQPATSRDRGA
                     GPFARMVLRGATVIDGTGAPPIGPIDIVVENGRIAEMRKVGVPKVPIKPERRPAAGEH
                     EIDCHGKYVTPGFIDCHAHAGVPYHAVHGWMPPVDYVYKLWLAHGVTTVREMGSFNGL
                     GWMLQQRALAEENAIAAPRLLSYVYIPVVNDMLKTLHTPEQVRAWLRRIKQRGADGVK
                     FFGAPPALMQAALDECGKLGLRSGCHHAQMAVTRMNALTSAGWGLSSAEHYYGLPEAL
                     FDDRIVQNYPTDYDYSDEYFRFAYAGQTFRQAAQPGSAKWNEVMEKFLALDFTFVPTF
                     TIYDANRDLMRTRQADWHHGYAWKSMWDFFQPSRGGHGSYWFRWSTTNEIEWKQNYQL
                     WMQFINEYKNRGGRVCTGSDSGFIFQLFGFGYIRELELLQEAGFHPLEVIRAATSQSA
                     DLVGLSDEIGSVEVGKCADLLVHDHNPLTDFKLLYGTGAMRLNDDTRKVEWHRALRYT
                     IKGGVVYDVEELLADVRALVERSYADTPEQRPGARLT"
BASE COUNT          409 a          881 c          793 g          428 t
ORIGIN      
        1 gaacgagccg ttgccggctt caccggcaaa tgcgcgaggg gcactgaaca ccgttccgac
       61 actctgcacc agcagcggcc tgctgcttcc cgtcacctgc accagccctt tgatgcgcag
      121 cagccgctcg ccacaccggc cggcgagatt atcgagccat tctgccagcg cctcccacgc
      181 aaccaccgca tccagccgcg caagcaccac gccgatccgt ggatgctgca atcccaccgc
      241 ttcggcgggc ggcagcaacc cgcgcaacga cggcggcgca cgcagcgaaa gcgcccccag
      301 cgccgctgca cggcggtcgt cggtgtcaat cagctcggct gtcgggttga tccccgctgc
      361 cagccggggt gcatcccgca gttgcgccgg gctggccagg tcacgcttgg ttaccaccag
      421 ggtttgcgcg cccgcccatt gcgccgcggc ctcctcgaag ttccccagcg tcgccccgcg
      481 cgtgcaatcg aaggtggcga caacccccac gcgcaggcca agcgacccca gcgacgccat
      541 ggaacgcagg atcgggccgg gcttggccag cccgctggtt tccagaatca tgcgtgacgg
      601 cggcgcaagt ccggcgtcct gacgcacggc cagcatgcct gcgatcgtcc tttccaggtc
      661 gctgccgagc gaacagcaaa cgcagccgtt gctgagcagc gcgaccggag aatcgccgcg
      721 ctccgccagg agcgcaccgt ccaggcccat ctggccgacc tcgttgacga tcacgcccgt
      781 atctgccgcc tctggcaacg ccaggaagtc acgcaacagc gtcgtcttgc cgctgccgag
      841 aaacccggtg aggacgatga agtcgatcgg cgtcacgtca gacgggcacc cggccgttgt
      901 tccggcgtgt cggcatagct gcgctccacc agcgcgcgca cgtcggcgag caattcctcc
      961 acatcataca cgacgccgcc ctttatcgtg tagcgcagcg cgcggtgcca ttcgaccttg
     1021 cgcgtgtcgt cgttcagccg catcgcgccc gtaccgtaca gcagcttgaa gtcggtcagc
     1081 ggattgtggt cgtgcaccag caggtcggcg cacttgccga cctcgacgct gccgatctca
     1141 tcgcttagcc cgaccaggtc ggcgctttgc gatgtggcgg cgcggatcac ttccagcggg
     1201 tggaatccgg cttcctgcag cagttccagt tcgcggatat agccgaagcc gaacaactgg
     1261 aagatgaaac cggaatcgct accggtgcag acccggccgc cgcggttctt gtactcgttg
     1321 atgaattgca tccagagctg atagttctgc ttccactcga tctcgttggt ggtggaccag
     1381 cggaaccagt acgacccgtg cccgccgcgg cttggctgga agaagtccca catcgacttc
     1441 caggcatagc cgtgatgcca gtcggcttgc cgcgtgcgca tcaggtcgcg gttggcgtcg
     1501 tagatcgtga aggtcggcac gaaggtgaag tccagcgcga ggaacttctc catcacctcg
     1561 ttccatttcg ccgatcccgg ctgcgccgcc tggcggaagg tctggccggc ataggcgaaa
     1621 cggaaatact cgtcgctgta gtcgtagtcg gtcggatagt tctgcacgat gcggtcgtcg
     1681 aacagcgcct ccggcaggcc gtaatagtgc tcggcgctgc tcagccccca gccggcactg
     1741 gtcagcgcgt tcatccgcgt caccgccatc tgcgcatgat ggcagccgga gcgcaggccc
     1801 agcttgccac actcgtccag cgccgcctgc atcagcgccg gcggtgcacc aaagaacttc
     1861 accccgtcgg cgccgcgctg cttgatccgg cgcagccagg cgcgcacctg ctccggcgtg
     1921 tggagcgtct tcagcatgtc gttgacgacg ggaatataca cgtaggacag caggcgcggt
     1981 gcggcgatcg cattctcctc cgccagcgcg cgctgctgca gcatccagcc gagtccgttg
     2041 aaggagccca tttcgcgcac tgtcgtgacg ccgtgcgcga gccatagctt gtagacgtag
     2101 tccaccggcg gcatccagcc gtgcaccgcg tgatagggca cgccggcatg cgcgtggcaa
     2161 tcgatgaagc ccggggtgac gtatttgccg tggcagtcga tttcgtgctc gccggcggcg
     2221 ggacggcgtt ccggcttgat cggcactttc ggcacgccga ccttgcgcat ctcggcgatg
     2281 cggccgttct ccaccacgat gtcgatcggg ccgatcggcg gggccccggt gccgtcgatc
     2341 accgtggcgc cccgcagcac catgcgggcg aaagggccgg cgccgcggtc gcggctggtg
     2401 gccggttgca gcgccggcat gccctgctgc gcccgctgtg ccgtcaattc ctcgccggca
     2461 tccggttcga atgtgtcgtc cgccatggtc aggctcctcg gatcaggcca a
//
LOCUS       DASSZH010000558         6089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_319947, whole genome shotgun sequence.
ACCESSION   DASSZH010000558 DASSZH010000000
VERSION     DASSZH010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319947"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1386)
                     /locus_tag="VFN42_12130"
     CDS             complement(<1..1386)
                     /locus_tag="VFN42_12130"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HET8997405.1"
                     /translation="MNDQTPPPQPAAAEQSSEAAPIGWEPELAELRQRIALAKELGGK
                     DRVERQHKGGRLTVRERIDRMLDPESFHEIGSIAGKATYDADSREILSFTPANCVFGR
                     GTVDGRPVVVAGDDFTLRGGSADASIKGKPTMSEEMAAAYRMPIIRLIEGSGGGGSVK
                     TIETTGRANLPGGLTQTTSFDLVAHQMGIVPVVALGLGSVAGLGAARLAASHYSVMVK
                     EMSAVFVAGPPVVERLGEPRTKQELGGWEIQLGCGAVDHAVDTEEDAFDAARRFLSYL
                     PSSIHDLPPRVRLWDDPDRRDEALIAAVPRDKKQAYHMRRIIEAVVDRGSFFEMGRLF
                     GRPIITGFARLDGWPVAVMAGDPLYHGGAWTADACSKIIRFVDMAQTFHLPIVHMVDC
                     PGFQVGLAAESSAVIRWGVRALSAINQTTVPWCSIVIRNCYGVGGLGHQPVGHYCVRY
                     AWPSASWGSLPL"
     gene            complement(1467..2279)
                     /locus_tag="VFN42_12135"
     CDS             complement(1467..2279)
                     /locus_tag="VFN42_12135"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HET8997406.1"
                     /translation="MTETWRDTHDWGALTRAEIAAARDAGALPVLTVGSVEQHADHLP
                     VDTDTLSAYQVALRAAERCPAPHVLVLPPPSFGFSPHHRSWAGTITLSLETFVGMVTD
                     VAESLHRTGFKRLLIVNGHGGNQGPLTSACTALASRGLEVGFLNYFAPAQKQWLEMLP
                     GAHRGIAHACAFETALQLALRPPALADKIAGRIKDLPPRLSPSYVAGNAPDVLRPAGA
                     YWAAIFTPGDVGYIGDPAAATRESGEAMTEACIAGLAQFFADFASARLRVGS"
     gene            2347..4434
                     /locus_tag="VFN42_12140"
     CDS             2347..4434
                     /locus_tag="VFN42_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HET8997407.1"
                     /translation="MQATRDIRASVCPHDCPSTCALSVEVLDGTRIGAVHGAEDNSYT
                     AGVICNKVSRYAERIHHPDRLTHPLLRTGPKGSTQFRRISWEEALDRVAERFTELAEK
                     HGAEAIWPFYYAGTMGLVQRDGINRLRHVMRYSRQKLTICTSLPEAGWCAGVGQSRGV
                     DAREMAQSDLIVMWGGNPVSTQVNVMTHITRARKERGAKLVVVDPYRSPTAAVADIHI
                     APKPGTDGALACAVMHVAFRDGHADRVYMARYTDCPDALEAHVRTRGPEWAAGITGLP
                     VAEIEAFAKLYCRTPRSYIRCGYGFARSRNGAANMHAVSCLPAVTGAWQHPGGGALWS
                     NRGMYHWDKTLIEGLDRRDLRVRELDMSRIASVLTGDRRDLGDGPEVHALLIQNQNPL
                     VVCPDTNRVRRGFSREDLFVCVHEQFMTETARWADIVLPATMFMEHDDLYQAGGHTHI
                     QIGPKLIEPPGECRSNHAVLQGLAQRLNAQHPGFAMTEMEIIDATLRASGWPDAATVL
                     RERWIDGALPFDQAHFLSGFPTADKKFHFAPAWAAMGDTRGVMPKLPDHMPAFEHTSD
                     ACPFRLMTSPARSFLNTSFTEMASGRKREGRPTVMLHPDDAARLEIADGDAVKLGNAR
                     GEVVLHARVTGGMQPGVLVAESIWPSEAFEGGIGINALTSDDPGPPMGGAVFHDTAVW
                     LRPATAALRLAAE"
     gene            complement(4446..>6089)
                     /locus_tag="VFN42_12145"
     CDS             complement(4446..>6089)
                     /locus_tag="VFN42_12145"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HET8997408.1"
                     /translation="DLQAAGMGFYIENIATDFYAAYHRWFPGKPVNWRFVDAQQRYRA
                     DPTDRRLLFREPSLSDPVWLARIRARLMTTVRVYRADHPLYYNLADESGIADLTAFWD
                     FDLSPESVAGMRHWLRQQYGSLAALNAEWGSQFQSWDAVQPETTRQAMQRHDGNYAAW
                     SDFKAWMDLSYARALRAGTDAVHAADPQAVAGMEGVQVPGWGGFNYADLAHAVDLMEV
                     SGFGNESLPLLQSLNPRLIPLVTTGGSDPGSLRSIWYATLNGVRGLVLWDGSDSIVRP
                     DGTLGPDGKAFAPAFAALHRVAPVLSASTPVWDPVAILYSPESFRLQWMLDQQPKGDA
                     WMRRKSETELESNAWRRALRGYLDSLRGLHLRPRFVTASMLPGLRARVLILPDTLSLS
                     PQAARAITRFAAQGGLVIADRQPGEFDGHGKRLAQPDLPLGIVHLVPPSDRAALRRSL
                     APAGVMPAIKLDDPDGSSASDVWLYLYRHGDGFVLALERRDGGAAPRSVVLRLPPGTT
                     VLRRVLGGKVERAGNRITVALPPVEPTILLLASTSHQPP"
BASE COUNT          997 a         2116 c         2057 g          919 t
ORIGIN      
        1 caggggaagc gagccccagc ttgccgaagg ccaggcatag cgcacgcagt aatgcccgac
       61 cggctggtgg ccgagtccgc ccacgccgta gcagttgcgg atcacgatgc tgcaccaggg
      121 cacggtggtc tggttgattg cggacagcgc gcgcacgccc caacggatca ccgcgctgct
      181 ctccgcggcc aggccaacct ggaaaccggg acagtccacc atgtgcacga tcggcagatg
      241 gaaggtctgc gccatgtcga cgaagcggat gatcttgctg cacgcatccg cggtccaggc
      301 gccgccatgg tacagcggat cgcccgccat caccgccacc ggccaaccgt ccagccgagc
      361 gaagccggtg atgatgggcc ggccgaacag gcggcccatt tcgaagaagg agccgcgatc
      421 caccaccgcc tcaatgatgc ggcgcatgtg gtaggcctgc ttcttgtcgc gcggcactgc
      481 ggcgatcagc gcttcgtccc gccgatccgg atcgtcccac aggcggacgc gcggcggcag
      541 gtcgtggatg gagctgggca ggtacgacag gaagcgccgg gcggcgtcga aggcgtcctc
      601 ttcggtgtcc accgcgtggt ccaccgcgcc gcagcccagc tggatttccc agccgccgag
      661 ttcctgcttg gtgcgcggct cgcccagacg ttccaccact ggcgggccgg cgacgaacac
      721 cgcggacatc tccttcacca tcaccgagta atggctggcg gccagccgcg ccgcgcccag
      781 cccggcgacc gatcccagac ccagcgccac cacgggcacg atgcccatct ggtgggccac
      841 cagatcgaac gaggtggtct gcgtcagtcc gccaggcaga ttggctcggc cggtggtctc
      901 gatcgtcttc accgagccgc cgccgccgga accttcgatc agccggatga tcggcatacg
      961 gtaggcggcc gccatttcct ccgacatcgt cggtttgccc ttgatcgagg catccgctga
     1021 tccgccgcgc agggtgaagt catcgccggc gaccaccacc gggcggccat cgaccgtgcc
     1081 gcggccgaat acgcagttgg ccggggtgaa ggacaggatc tcgcggctgt cggcgtcata
     1141 cgtggccttg ccggcgatgc tgccgatctc gtggaagctc tccgggtcca gcatgcggtc
     1201 gatgcgttcg cgcacggtga gccggccgcc cttgtgctgc cgctccaccc ggtccttacc
     1261 gccaagttcc ttagcgagcg cgatgcgctg ccgcagctcc gcgagttcgg gttcccagcc
     1321 gatcggcgcg gcttcgctgc tctgctctgc cgccgccggc tgcggcggtg gcgtctggtc
     1381 gttcatgccg aaggcgttcc ctgtccgtcc tttgggtttg ggacgctagg gaggaattgg
     1441 cgcatgcgca acggcggggc cggccgtcag ctacccaccc gcagccgtgc gctggcgaaa
     1501 tcggcgaaga actgcgccag gccggcgatg caggcttcgg tcatcgcttc ccctgactcg
     1561 cgtgtcgccg ccgccgggtc gccgatgtat cccacgtcgc ccggcgtgaa gatcgccgcc
     1621 caatacgcac ccgccgggcg cagcacgtcg ggcgcgttgc ccgcgacata ggacggcgac
     1681 agccgcggtg gcagatcctt gattcgccct gcgatcttgt ccgccagtgc gggcggacgc
     1741 agcgccagct gcagcgccgt ctcgaaggcg caggcatgcg cgatgccgcg atgcgcgccg
     1801 ggcagcatct ccagccactg cttctgcgcc ggcgcgaaat agttcaggaa gccaacctcc
     1861 aatccccggc tggccaacgc ggtgcaggcg gatgtcagcg gcccctggtt gccaccatgg
     1921 ccgttgacga tcagcagccg cttgaagccg gtgcggtgca ggctctccgc cacatccgtc
     1981 accatgccga cgaaggtttc cagcgacagt gtgatggtcc cggcccagct tcggtgatgc
     2041 ggcgagaacc cgaaggacgg cggcggcagc accagcacat gcggcgccgg gcagcgctcc
     2101 gccgcgcgca gcgccacctg gtaggcggac agcgtatcgg tatccaccgg caggtggtcg
     2161 gcatgctgtt ccaccgagcc caccgtcagc accggcagcg cgccggcgtc gcgggcggcg
     2221 gcgatctccg cacgggtcag cgcgccccag tcatgcgtgt cgcgccaggt ctcggtcatc
     2281 ggccggtctc cctcgttgcc ggaacacttt gccaagccgg ccgcggcgcg gctaggcttc
     2341 cacgccatgc aagccacacg cgacatcagg gcctcggtct gcccgcacga ctgcccatcc
     2401 acctgcgcat tgtcggtgga ggtgctggac ggcacccgca tcggtgcggt gcacggcgcc
     2461 gaggacaaca gctacacggc tggcgtgatc tgcaacaaag tgtcgcgcta tgcggagcgt
     2521 atccatcatc cggaccggct gacgcatccg ctgctgcgca ccggcccgaa gggatccacc
     2581 cagttccgcc gcatctcctg ggaggaggcg ctggaccgcg tcgccgagcg gttcaccgaa
     2641 ctggccgaga agcacggcgc ggaggccatc tggccgttct attatgccgg cacgatgggg
     2701 ctggtgcagc gcgacggcat caaccgcctg cgccacgtga tgcgctactc gcggcagaag
     2761 ctgaccatct gcacgtcact gccggaggcc ggctggtgcg ccggcgtcgg gcagagccgc
     2821 ggcgtcgatg cgcgcgagat ggcacagtcc gacctgatcg tgatgtgggg cggcaatccg
     2881 gtctccaccc aggtgaacgt gatgacgcac atcacccgcg cgcggaagga gcgcggcgcg
     2941 aagctggtgg tggtcgatcc ataccgttcg ccgactgcgg ccgtggcgga tatccacatc
     3001 gcgccgaagc ccggcacgga cggcgcgctg gcctgtgcgg tgatgcacgt ggcgttccgc
     3061 gacggccatg ccgaccgcgt gtacatggcg cgctacaccg attgcccgga cgcgctggaa
     3121 gcgcatgtgc gcacacgtgg cccggaatgg gcggcgggga ttaccggctt gccggtggcg
     3181 gagatcgaag ccttcgccaa gctgtattgc cgcacgccgc gcagctacat ccgctgcggc
     3241 tacggcttcg cgcgcagccg caacggcgcg gcgaacatgc acgccgtgag ctgcctgcca
     3301 gcggtcaccg gcgcctggca gcaccccggc ggcggggcgc tgtggtccaa ccgcggcatg
     3361 tatcactggg acaagacact gatcgagggc ctggatcgcc gcgacctgcg ggtgcgcgag
     3421 ctggacatga gccgcatcgc cagcgtgctg accggcgacc gccgcgacct gggcgacggc
     3481 ccggaggtgc atgcgctgct gatccagaac cagaacccgc tggtggtctg ccccgacacc
     3541 aatcgcgtgc gccgcggctt ctcgcgcgag gacctgttcg tctgcgtgca cgagcagttc
     3601 atgaccgaga ccgcgcgctg ggccgacatc gtgctgccgg cgacgatgtt catggagcac
     3661 gacgacctgt accaggctgg cggccacacc cacatccaga tcggccccaa gctgatcgag
     3721 ccgccgggcg aatgccggtc caaccatgcg gtgctgcaag gcctggcgca gcgcctgaac
     3781 gcgcagcatc ccggcttcgc gatgaccgaa atggagatca tcgacgccac gctgcgcgcc
     3841 tccggctggc cggacgcggc gacggtgctg cgggagcgct ggatcgacgg cgcgctgccg
     3901 ttcgatcagg cgcatttcct cagcggattt ccgaccgccg acaagaagtt ccatttcgcg
     3961 ccggcctggg cggcgatggg cgatacgcgc ggggtgatgc cgaagctgcc ggaccatatg
     4021 ccggctttcg aacacacgtc ggacgcatgc ccgttccggc tgatgacctc tccagcgcgg
     4081 agctttctca acaccagctt caccgaaatg gcatccggcc ggaaacgcga gggccggccg
     4141 accgtgatgc tgcatccgga tgacgccgcc cggctggaaa tcgctgacgg cgatgcggtg
     4201 aagcttggca atgcgcgcgg tgaagtggtg ctgcacgcgc gcgtcaccgg cggcatgcag
     4261 cccggcgtgc tggtcgcaga aagcatctgg ccgagcgaag cgttcgaggg cgggatcgga
     4321 atcaatgcac tgaccagtga cgatccggga ccgccgatgg gcggtgcggt gttccacgac
     4381 accgccgtgt ggctgcgtcc ggccaccgcg gcactgcgcc tggccgcgga gtagcgcgca
     4441 ggatcctacg gcggctggtg ggacgtcgaa gccagcagca ggatggtcgg ctcgaccggc
     4501 ggcaaggcca cggtgatccg attccctgcg cgctctacct tcccgcccag cacgcggcgc
     4561 agcaccgtgg tgccgggcgg caggcgaagc acgacgctac gtggtgccgc cccgccatcg
     4621 cggcgttcca gcgccaggac aaacccgtcg ccatgccggt agaggtatag ccacacatcc
     4681 gaggcgctgc tgccatccgg gtcatccagc ttgatcgccg gcatcacccc ggctggcgcg
     4741 agcgatcgac gcaatgccgc ccgatcgctc ggcggtacca gatgcacgat gccgagcggc
     4801 aggtccggtt gcgccagccg cttgccgtgg ccatcgaatt ccccgggctg ccggtcggcg
     4861 atcaccagcc cgccctgagc ggcgaagcgc gtgatggcac gcgccgcctg cggcgacagg
     4921 ctcagcgtgt ccggcaggat caacacgcgt gcccgcagcc ctggcaacat cgacgccgtg
     4981 acgaagcggg gacgcagatg caggccgcgc agcgagtcca gataaccgcg caacgcacgc
     5041 cgccaggcat tgctttccaa ttcggtctcg gacttgcgcc tcatccacgc gtcgcccttc
     5101 ggctgctggt ccagcatcca ttgcagccgg aaactctcgg gcgaatacag gatggccacc
     5161 gggtcccaga ccggcgtact ggcgctcagt accggtgcga cgcgatgcag cgcggcaaag
     5221 gccggggcga aggccttgcc atccgggccg agcgtaccgt ccgggcgcac gatcgagtca
     5281 ctgccgtccc atagcaccag accgcgtacg ccgttcagcg tggcgtacca gatgctgcgc
     5341 aagctgcccg ggtcgctgcc gcccgtggtg acgagcggaa tcagccgcgg attcagcgat
     5401 tgcagcagcg gcaggctttc gtttccgaag ccgctgacct ccatcaggtc aaccgcatgc
     5461 gccagatcgg cgtaattgaa gccgccccag ccgggaacct gaacgccctc cattccggca
     5521 accgcctgtg gatcggccgc gtgcaccgcg tccgtgccgg cccgcaacgc acgcgcatag
     5581 gagagatcca tccaggcctt gaagtcggac caggcggcgt aattgccatc gtgccgctgc
     5641 atggcctgcc gcgtcgtttc cggctgcacg gcgtcccagc tttggaactg cgaaccccat
     5701 tcggcattca gcgcggccag cgagccgtat tgctgccgta gccagtgtcg catgccggcg
     5761 accgactcag gcgacagatc gaaatcccag aatgccgtga gatcggcgat gccggattcg
     5821 tctgccagat tgtaatatag cgggtgatcg gcgcggtaca cgcgcaccgt cgtcatcagc
     5881 cgtgcacgga tacgcgccag ccagaccgga tcggacaggc tgggctcgcg gaacagcaga
     5941 cggcgatccg tcggatcggc gcggtagcgc tgctgcgcat ccacaaaccg ccagttgacc
     6001 ggcttgccgg ggaaccagcg atggtaggcg gcatagaaat ccgtcgcgat attctcgatg
     6061 tagaagccca tgccggcggc ttgcaggtc
//
LOCUS       DASSZH010000559         4199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_712428, whole genome shotgun sequence.
ACCESSION   DASSZH010000559 DASSZH010000000
VERSION     DASSZH010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712428"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..1630)
                     /locus_tag="VFN42_12150"
     CDS             complement(221..1630)
                     /locus_tag="VFN42_12150"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF004815.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET8997409.1"
                     /translation="MDTEALCFTPATQLAELIRTKQVSPVEYMRAVLDRIQALDPQVN
                     AFAHLAAEQAMEAARQAEKALLGGERIGRLHGIPVTIKDLAITKDMPTQSGSRIYAGH
                     QPSEDAPMVPRLRDEGAIILGKTTTSEFGWTGVSRSPLTGITHNPWKHGYNAGASSAG
                     AGAAAAAGFGPLHQGSDGAGSIRMPSHFCGVFGLKPSFGRVPNYPVSTGDFTSHNGPM
                     ARTVADTALLLEVLSGPHPLDFTTLEAGPAHYVARLQEGIRGKRIAYSPDLGHARVDP
                     EVAALVEKAVARFSELGATVEQVPTPWAKDGPELVRFFWAAHLTRLQPQLAKWESQMD
                     PGLVACIRDAQNYSVSEYQLMRERKMAYVAAIHRWFQDWDFLLTPAVSVAAFPAERLM
                     PEHWPQHSWDWVMWAEFSYPFNMSWNPAASVPCGFTAEGLPVGLQIVGRRFDDLGVLQ
                     AAAAFEQVQPWADKRPSLG"
     gene            complement(1656..2384)
                     /locus_tag="VFN42_12155"
     CDS             complement(1656..2384)
                     /locus_tag="VFN42_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8997410.1"
                     /translation="MAGRFTGRTAIVTGGVSGIGAGIAARLAAEGARVSLWDRDAAAL
                     ERADAPHKIALDVTDAAAVQHAAEATAAMLGRIDILIASAGITGPNHPTWDYPVAEWD
                     RVIDVNLKGVFYCNRAVVPFMQRDGYGRIVNIASIAGKEGNPNASAYSASKAGVIALT
                     KALGKELAQTEIRVNCVTPAAVRTPIFDQMTQQQIDWMLSKIPVGRFGEIEEIAHLVC
                     WLASEECSFSTGAVFDLSGGRATY"
     gene            2514..2639
                     /gene="ykgO"
                     /locus_tag="VFN42_12160"
     CDS             2514..2639
                     /gene="ykgO"
                     /locus_tag="VFN42_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007281615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HET8997411.1"
                     /translation="MRIRNSLKSAKVRDKNCRVVRRRGRVYVINKKNPRMKARQG"
     gene            complement(2745..3575)
                     /locus_tag="VFN42_12165"
     CDS             complement(2745..3575)
                     /locus_tag="VFN42_12165"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET8997412.1"
                     /translation="MIQHLSGPLGTMDESGAAGSDRPGDSKPMQSEPDLLRHSPRALH
                     EQLTDRLRAELASSYRAGDRIPTEGEIMQAHRLSRVTVRRALQTLVDDGVLVRRQGKG
                     TFLASQRPRVVHRIDRFGPFLDAFTASGVEVQVTLLDFRWEGPGALPDVLRAGQRKAL
                     VYDRLYATDGFAHGLNRITLPGEFGEAVTTRHAETMGIYQILREVLGIVPQRAEFELI
                     SELPGTALARALRISPSTPLLVLDRISFDASGRAVERTRHHLLPEVYRLAFGLAPGPG
                     "
     gene            3652..>4199
                     /locus_tag="VFN42_12170"
     CDS             3652..>4199
                     /locus_tag="VFN42_12170"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997413.1"
                     /translation="MSKQNTRIAGETAGAAAGGRFGRRAAIGGALGLAASVAAPHIAR
                     AAGKYTLRLASWGSPNAPQVAAFVTQFQKLVAGNSHGQIEVQDFPAGSLVSERAVPSA
                     IQTRVVDIALTTMGSWASIAQSAGALNTVFFSPTGDRFEKIIGPGTPLFHTFDADMAT
                     HGVRVLAALYNGPVVVVSNASMA"
BASE COUNT          653 a         1449 c         1425 g          672 t
ORIGIN      
        1 gccgctgatc gggttgacga tggagaacag cgccgggaag gcgagcagga aaaccttgat
       61 ggcctcggtc atgggggcgc gaaaaacaaa ttccccctcc ccttgcggga gggggtcagg
      121 gggaggggcg gtgcattccg ctccaaggcc aggatggatc gcacgccccc tcccctcatc
      181 cccctcccag gcggggaggg ggagtttcgt gcctctcgcg tcaccccagg ctcggccgct
      241 tgtcggccca gggctggacc tgctcgaacg ccgcggcggc ctgcagcacg cccaggtcgt
      301 cgaaccgccg gccgacgatc tgcaagccta ccggcaggcc ctcggccgtg aagccgcaag
      361 gcacgctggc ggccgggttc caggacatgt tgaacgggta ggagaactcc gcccacatca
      421 cccagtccca ggaatgctgc ggccagtgct ccggcatcag ccgctctgcc gggaaggcgg
      481 cgaccgagac cgcgggggtc agcaggaagt cccagtcctg gaaccagcga tggatcgccg
      541 cgacataggc catcttgcgt tcgcgcatca gctggtactc ggagaccgag tagttctgcg
      601 cgtcgcggat gcaggcgacg aggcccgggt ccatctgcga ttcccatttc gcaagctgcg
      661 gctgcagccg ggtcaggtgg gctgcccaga agaagcggac cagctccggc ccgtccttgg
      721 cccagggcgt cggcacctgc tccaccgtgg caccgagttc gctgaaccgc gccaccgcct
      781 tctccaccag cgccgccact tccgggtcga cgcgggcgtg ccccaggtcc gggctatagg
      841 cgatgcgctt gccacgaatg ccttcctgca ggcgcgccac gtaatgggcc gggccggctt
      901 ccagcgtggt gaaatccagc gggtgcgggc cggacagcac ctccagcagc agggccgtgt
      961 cggcgaccgt gcgggccatc ggcccattat gcgaggtgaa gtcgccggtg ctgaccggat
     1021 aattcggcac gcggccgaag ctcggcttca gcccgaacac gccgcagaaa tgcgacggca
     1081 tgcggatgga gccggcgccg tcgctgccct ggtgcagcgg cccgaagccc gccgccgcgg
     1141 cagcacccgc cccggccgag gacgcaccgg cattgtagcc gtgcttccat gggttgtggg
     1201 tgatgccggt cagcgggctg cgcgagacgc cggtccagcc gaactccgag gtggtggtct
     1261 tgcccaggat gatcgcgccc tcgtcgcgca ggcggggcac catcggcgcg tcttcggacg
     1321 gctggtggcc ggcgtagatc ctgctgcccg actgggtcgg catgtccttg gtgatcgcca
     1381 ggtccttgat ggtcaccggg atgccgtgca gccggccgat gcgttcgccg cccagcagcg
     1441 ccttctcggc ctgcctggcc gcctccatcg cctgctccgc ggccaggtgg gcgaaggcat
     1501 tcacctgcgg gtccagcgcc tggatgcgat ccagcaccgc ccgcatgtat tcgaccggcg
     1561 acacttgttt ggtacggatc agttcggcga gctgcgtcgc cggggtgaag cagagcgctt
     1621 cggtatccat tcccttggtc tcctctggca ccgtttcagt atgtcgcacg tccgccggac
     1681 aggtcgaata ccgcgccggt ggagaagctg cattcctcgc tggccagcca gcacaccaga
     1741 tgggcgattt cctcgatctc gccgaaccgg ccgaccggga tcttcgacag catccagtcg
     1801 atctgctgct gcgtcatttg gtcgaagatc ggcgtgcgca ccgcggcggg ggtgacgcaa
     1861 ttcacccgga tctccgtctg cgcgagttcc ttgccgagcg ccttggtcag cgcgatgacg
     1921 ccggctttgg aggcggaata ggccgaggcg ttcgggttgc cctccttgcc ggcgatcgag
     1981 gcgatgttga cgatccgccc gtagccatcg cgctgcatga agggcaccac cgcgcggttg
     2041 cagtagaaca cgcccttcag gttgacgtcg atcacccggt cccattcggc gactgggtag
     2101 tcccaggtcg gatgattggg gccggtgatg ccggccgagg cgatcaggat gtcgatgcgc
     2161 cccagcatcg ccgcggtggc ttccgcggca tgctgcacgg cggccgcgtc ggtgacgtcc
     2221 aacgcgatct tgtgcggggc gtcggctcgt tccaatgctg ccgcatcgcg gtcccacaac
     2281 gacacccgcg ccccctcggc cgccagccgc gcggcgatgc cggcgccgat gccggaaacg
     2341 cctccggtca cgattgccgt ccgtccggtg aaccgacctg ccatgcggga tctcctgctg
     2401 tgccggtgct atgaaggacg gcaggggctg cgagggcaag ccgcgccttg actccccgcc
     2461 gcctgccccc tatccaccgc cgctcgcatt tttctgccgg aaagcttgcc cctatgcgta
     2521 ttcggaacag cctcaagtcc gcgaaggtcc gcgacaagaa ctgccgtgtc gtgcggcggc
     2581 gggggcgggt ttatgtgatc aacaagaaga acccgcggat gaaggcccgc cagggctgag
     2641 ctagcggtaa ccccggcctg ctgcactgat ccggcagccg gcgatcctgc gctcgacttg
     2701 agccgtgccg ctgccgctgg atggcatgcg gcggcgcccg aatctcatcc ggggcccggt
     2761 gccaggccga aggccaggcg atacacctcc ggcaacaggt ggtggcgggt acgctccacc
     2821 gcccggcccg acgcgtcgaa gctgatgcgg tccagaacca gcagtggcgt gcttggcgaa
     2881 atgcgcagcg cacgcgccag cgcggtgccg ggcagttcac tgatcagctc gaactcggcg
     2941 cgctgcggga cgatgcccag cacctcgcgc aggatctgat aaatccccat ggtttccgcg
     3001 tgccgcgtgg tcaccgcctc accgaactcg cccggcagcg tgatgcggtt caggccgtgc
     3061 gcgaacccgt cggtcgcata cagccggtca tagaccagcg ccttgcgctg acccgcgcga
     3121 agcacatccg gcagagcgcc cggcccctcc caacggaaat ccagcaaggt gacctggacc
     3181 tccacgcccg aggcggtgaa ggcatcgaga aacgggccga aacggtcgat ccggtgcacc
     3241 acgcgcgggc gctgactggc aaggaatgtc cccttgccct gacgccgcac cagcacgcca
     3301 tcgtccacca gggtctgcag cgcccggcgg acggtgaccc ggctcaggcg atgcgcctgc
     3361 atgatctccc cctcggtggg aatgcggtcg cccgcccggt agctgctggc cagctcggcg
     3421 cgcagccggt cggtgagctg ctcgtgcagc gcccgcgggc tgtgccgcag caggtcgggt
     3481 tcggactgca tcggcttgct gtccccaggc cggtcgctgc cggcagcgcc gctctcatcc
     3541 atggtgccga gtggccccga gaggtgttgt atcatcctgt tttgtatcat aacgtcttct
     3601 caggcatcaa cgctgccgcg aatggcagca cgcccggctg gggagagacg gatgagcaag
     3661 cagaacacca gaattgccgg cgagaccgcc ggcgcggcgg caggcggccg cttcggacgc
     3721 cgcgcggcga tcggcggagc actcgggctg gccgcgtcgg tcgcggcgcc gcacatcgcc
     3781 cgcgcggcgg gaaaatacac gcttcgcctg gcgagctggg gctcccccaa tgcgccgcag
     3841 gtggccgcct tcgtcacgca gttccagaag ctggtcgccg gcaattcaca cgggcaaatc
     3901 gaagtgcagg atttccccgc cgggtccctg gtgagcgaac gcgcggtacc ctcggcaatc
     3961 cagacccggg tggtggacat cgcgctcacg acgatgggca gctgggcatc gatcgcgcag
     4021 agcgcggggg cgctcaacac ggttttcttt tccccgaccg gcgaccgctt cgagaagatc
     4081 atcggcccgg gcacgccgct gttccacacc ttcgacgcgg atatggcgac gcacggcgtg
     4141 cgggttctcg ccgcgctcta caacggtccg gtcgtggtgg tttccaatgc ctccatggc
//
LOCUS       DASSZH010000560         2420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_933815, whole genome shotgun sequence.
ACCESSION   DASSZH010000560 DASSZH010000000
VERSION     DASSZH010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933815"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VFN42_12175"
     CDS             <1..838
                     /locus_tag="VFN42_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8997414.1"
                     /translation="RQQAMLAKEIAFIERFKARASHAAQVQSRVKKLDKIERVEPPKR
                     RQTVEFAFPPAPRSGEDVAHLRGVHKRYGSRVIYQGLDLLLRRRERYCVLGVNGAGKS
                     TLLKLVAGAAEPDAGSVTLGGSVKLGYFAQHAMDLLDGERSVFDTLNDAFPRAAQGSL
                     RALAGGFGFSGDEIDKPCRVLSGGEKARLVMALMLYEPPNFLVLDEPTNHLDIATKQM
                     LIGALGQYEGTMLFVSHDRHFLAALSNRVLELTPEGVQHYGGGYTEYVARTGREAPGL
                     HG"
     gene            complement(878..2056)
                     /locus_tag="VFN42_12180"
     CDS             complement(878..2056)
                     /locus_tag="VFN42_12180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET8997415.1"
                     /translation="MSEATMVALGEDYPELRESVRRICARYPGSYWSGLEEQEAYPTA
                     FVDELTQAGFLAALIPEEYGGSGLPLRAAAVILEEINASGCVASQGHAQMYIMGTLLR
                     HGSEAQKRKYLPEIAAGRLRLQAFGVTEPTTGSDTTQLKTRAVRDGDHYVVTGQKVWT
                     SRALYSDMMLLLARTTPADQVKKRSDGLSVFLVDLSEKNTGKGLEIRPIKAMINHNTT
                     EVFFDNFRIPASSLIGEEGRGFRYILDGMNAERVLVASEAVGDGRWFVKKATAYANDR
                     VVFGSPIGKNQAVQFPIARAWAELEAADMVCRRAAALFDDGKECGADANIAKLLASEA
                     CWKAADTCMQTYGGFSYAREYEIERKWRESRIYQIAPISTNLILGYVGQHVLGMPRSY
                     "
     gene            2158..>2420
                     /locus_tag="VFN42_12185"
     CDS             2158..>2420
                     /locus_tag="VFN42_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019091617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET8997416.1"
                     /translation="MRILFVTSNRLGDAVLSTGLLDHLLRVYPEARFTVACGPVAEGV
                     FERMPRRDRTIVLRKQPYGRHWLPLWAATVTRRWDLVVDIRGSA"
BASE COUNT          362 a          803 c          831 g          424 t
ORIGIN      
        1 ccggcagcag gcgatgctgg ccaaggagat cgccttcatc gagcgcttca aggcgcgcgc
       61 gtcacatgcg gcgcaggtgc aaagccgggt gaagaagctg gacaagatcg agcgggtgga
      121 accgccgaag cgccgccaga cggtggaatt cgcctttccg ccggcgccgc gttcgggtga
      181 ggacgtggcg catctgcgcg gcgtgcacaa acgctacggc agccgggtga tctaccaagg
      241 gctggacctg ctgctgcgcc ggcgcgagcg gtactgcgtg ctgggcgtga acggtgcggg
      301 caaatccacg ctgctgaaac tggtcgccgg cgcggcggaa cccgatgccg gcagcgtcac
      361 ccttgggggc agcgtgaagc tggggtattt cgcccagcac gcgatggatc tgctggatgg
      421 cgagcgcagc gtgttcgaca cgctgaacga tgcgtttccg cgcgccgcgc agggctcgct
      481 gcgtgcgctg gccggcggct tcggcttctc cggcgatgag atcgacaagc cgtgccgcgt
      541 gctgtcgggc ggcgagaagg ctcggctggt gatggcgctg atgctgtatg agccgccgaa
      601 tttcctggtg ctggacgagc cgaccaacca cctggacatc gccaccaagc agatgctgat
      661 cggcgcgctg gggcagtatg agggcaccat gctgttcgtc tcgcacgacc ggcatttcct
      721 tgccgcgctg tccaaccgcg tgctggaact cacgccggag ggtgtgcagc actatggcgg
      781 cggctacacg gagtatgtcg cgcgcaccgg gcgggaagcg ccggggctgc acggttaggg
      841 cacgcgtacc ccggcggggt acgcgctcac caccgcctca atacgagcgc ggcatgccca
      901 gcacgtgctg gccgacatag cccaggatca ggttcgtgga gatcggcgcg atctggtaga
      961 tgcgcgactc gcgccatttg cgctcgatct cgtattcccg cgcatagctg aacccgccat
     1021 aggtctgcat gcaggtatcc gccgccttcc agcaggcttc gctggccagc agcttggcga
     1081 tgttggcgtc ggcgccgcac tccttgccgt cgtcgaacag cgccgctgct cgcctgcaga
     1141 ccatgtccgc cgcctccagt tccgcccagg cgcgggcgat cgggaactgc accgcctggt
     1201 tcttgccgat cggtgacccg aacaccacgc ggtcgttcgc ataggcggtg gccttcttca
     1261 cgaaccagcg gccgtcgccg accgcctcgc tcgccaccag cacgcgttcg gcgttcatgc
     1321 cgtccaggat gtagcggaag ccgcggcctt cctcgccgat caggctggat gccgggatgc
     1381 ggaaattgtc gaagaacacc tcggtcgtgt tgtggttgat catcgccttg atcgggcgga
     1441 tctccagccc cttgccggtg ttcttctcgc tgagatccac cagaaagacg gaaagcccgt
     1501 cgctgcgctt cttcacctgg tcggccggcg tggtgcgcgc cagcagcagc atcatgtcgg
     1561 aatacagcgc gcgcgaggtc cacactttct gtccggtcac cacgtaatgg tcgccgtcgc
     1621 gcaccgcgcg ggttttcagc tgcgtggtgt ccgaaccggt ggtcggctcg gtcacgccga
     1681 acgcctgcag ccgcaggcgg ccggcggcga tctccggcag gtatttgcgc ttctgtgcct
     1741 cgctgccgtg ccgcagcagc gtgcccatga tgtacatctg cgcatggccc tggctggcga
     1801 cgcagccgct ggcgttgatc tcctccagga tcaccgcggc ggcgcgcagc ggcaggccgg
     1861 aaccgccgta ctcttccggg atcagcgcgg ccaggaaacc ggcctgggtg agttcgtcga
     1921 cgaaagcagt ggggtacgcc tcctgctcct ccagtccgct ccagtagctc ccgggatagc
     1981 gcgcgcagat gcggcgcacg gattcgcgca gttcgggata atcctcgccg agcgcgacca
     2041 tcgtggcttc cgacatgatc ccctccgtgc tgagcggggg cacgctgccc aaccgcgtgg
     2101 caagcgtcaa acggtcgcct cgcgccgatg gtgcaacgct gctatggggc agcggcaatg
     2161 cgcatcctgt tcgtgacctc caaccggctg ggcgatgcgg tgctctccac cgggttgctg
     2221 gaccacctgc tgcgcgttta cccggaggcg cgcttcaccg tggcctgcgg cccggtggcc
     2281 gagggcgtgt tcgagcgcat gccgcggcgc gaccgcacca tcgttctgcg caagcagccc
     2341 tatggccggc attggctgcc gctgtgggcc gcgacggtga cccggcgctg ggaccttgtg
     2401 gtggatatcc gcggttcggc
//
LOCUS       DASSZH010000561         3018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_371275, whole genome shotgun sequence.
ACCESSION   DASSZH010000561 DASSZH010000000
VERSION     DASSZH010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371275"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VFN42_12190"
     CDS             complement(<1..746)
                     /locus_tag="VFN42_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET8997417.1"
                     /translation="MSATPLDKIRNIGITAHIDAGKTTTTERILYYTGVSHKIGEVHD
                     GNTTTDYMEQERERGITITSAAVTCEWKDTRINIIDTPGHIDFNIEVNRSLRVLDGAV
                     FIIEGVAGVQPQSETNWRLADRYNVPRIIFINKLDRTGADFYRAFATLKEKLDIIALP
                     LQLPIGVEENFLGVVDLVEMKAIIWEGGELGAKFHDEPIPAELEEQAKEYRQNLLDTA
                     LAVDDAAMEEYFDKGDVSVETLKRCIKTGT"
     gene            1101..2381
                     /locus_tag="VFN42_12195"
     CDS             1101..2381
                     /locus_tag="VFN42_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010501339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HET8997418.1"
                     /translation="MRTAHALVRFGLGRRGTEPLPADPPGWLLEQLQRPDPARFAEPP
                     STVIGLTALRHDRETKPAPGESLSRKLYRTQAAAQLANAVTTPAPFRERLVWFWTNHF
                     TVSLRRGQCAALAGAFVEEAIRPHVTGRFSDMLVAVMCHPAMLLYLDNAGSVGPDSPA
                     GLRTHRGLNENLARECLELHTLSPDAGYTQADVTAFAKILTGWSVDMRADVPGFRYHP
                     AAHEPGTQTMLRQEFPPDQAGGVAALHFLADHPATHRFLATKLVRHFVADTPPADVVG
                     TIDTVLRDTGGNLGAAAAALVHLDAAWQPGGKLRTPQDYVIAAVRSLDLPPDTVPMLG
                     ILAGLGQPVWTAPAPNGWPDRAADWAAPEAMLRRIDWASGFAGRIGTRDVDDIAEITL
                     GPLLGRDTREAIRHAGSRRDAMTLLLTSPEFQRR"
     gene            2385..>3018
                     /locus_tag="VFN42_12200"
     CDS             2385..>3018
                     /locus_tag="VFN42_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997419.1"
                     /translation="MALLSRPLSRRHTLLGLATCASLGRTHLAMAAAPTERRLVVVIL
                     RGALDGMATVVPYGDPALAGLRGAILPPPPNQPDGLLDLGGFYGLHPALTGLYEMYKA
                     GQATLVHAVAGCYRVRSHFEAQDYLESGADHRMTSGWLNRTVAGLPGARSGGPEGDAV
                     AIGVSVPLLLRGPAQVANWAPHGFAQPDAALYGRIAALNAHDPVTGPAIAE"
BASE COUNT          474 a         1102 c          928 g          514 t
ORIGIN      
        1 atcgtgccgg tcttgatgca gcgcttcaga gtctcgaccg agacatcgcc cttgtcgaaa
       61 tattcctcca tggccgcgtc atcgacggcg agggctgtat cgagcaggtt ctggcggtat
      121 tccttcgcct gctcttccag ctccgccggg atcggctcgt cgtggaactt ggcgcccagt
      181 tcgccacctt cccagatgat cgccttcatc tcgaccaggt cgacaacgcc caggaagttt
      241 tcctcgacac cgatcggcag ctgcaacggc agcgcaatga tgtccagctt ttccttcagc
      301 gtggcgaagg cacggtagaa atcggcgccc gtgcggtcca gcttgttgat aaagatgatg
      361 cgcggcacgt tgtaacgatc ggccaggcgc cagttcgtct cggactgcgg ctgcacgccg
      421 gcgacgcctt cgatgatgaa caccgcgccg tccagcacgc gcaggctgcg gttcacttcg
      481 atgttgaaat cgatatggcc gggcgtgtcg atgatgttga tgcgggtgtc tttccactca
      541 catgtcaccg cggccgaagt gatggtaatc ccccgctccc gctcctgctc catgtaatcg
      601 gtggtggtgt tgccgtcatg cacctcaccg atcttgtgcg acacgccggt gtagtagaga
      661 atccgctccg tcgttgtcgt cttgcccgcg tcgatatgcg cggtgatacc gatgtttcta
      721 attttatcga gcggcgtagc agacacgatc aacctcctaa caacagacgg gcgggcatat
      781 cagaaagggc gcgtgaccca tgactggatc cggggaaccg ccccgcgcgc ccgcctataa
      841 tgccggccga acacaacagc cggttatgtg gacccgcaca tgcgacagac tcagcggttt
      901 tgcaagtgcg aattccgcgc gatcggcgca tagccgggtc tacatgtccc gcccgaaccg
      961 ccccaccgga ccgcgccccg accttacgcc cgacggcctc cgaacttccc ctgctcctct
     1021 ccttctgggc gcgcagtgat cctgccagac cattcctcac agtctgaaat gatccgctgc
     1081 gctataccgt tggcatggac atgcgaacag cccacgccct ggtgcggttc ggcttaggcc
     1141 gccgcggcac ggagccactt cccgccgacc cgcccggctg gctgctggag cagttgcaac
     1201 ggcccgaccc ggcgcgtttt gccgaaccgc ccagcaccgt catcggcctg accgcgctgc
     1261 gccacgaccg ggagacgaag cccgctcccg gcgagtcgct cagccgcaag ctgtaccgta
     1321 cccaggccgc ggcgcagttg gccaacgcgg tgacaacgcc ggcgccgttc cgtgaaaggc
     1381 tggtctggtt ctggaccaac cacttcaccg tctcgctccg ccgtggccag tgcgccgcgc
     1441 tggcgggggc attcgtggaa gaagcgatcc gcccgcatgt caccggccgg ttcagcgaca
     1501 tgctggtggc cgtgatgtgc catccggcga tgctgctgta ccttgacaac gcaggttcgg
     1561 ttggtccgga cagcccggcc gggctgcgca cccatcgcgg cctgaacgag aacctggccc
     1621 gcgagtgcct ggaactgcac accctcagcc cggacgccgg ctatacccag gccgacgtca
     1681 ccgccttcgc gaagatcctg accggctggt cggtggacat gcgggcggat gtgcccggct
     1741 tccgctatca cccagcggcg catgagccgg gtacgcagac catgctgcgg caggaattcc
     1801 cgccggacca ggccggcggt gtcgcggccc tgcacttcct ggcagaccac ccggcaaccc
     1861 accgcttcct ggcgaccaaa ctcgtccgcc atttcgtcgc cgacacaccg ccggcggacg
     1921 tggtcggcac gatcgacact gtgctgcgtg acaccggcgg caacctgggc gccgcggctg
     1981 ccgccctggt gcatctggat gccgcctggc agcccggcgg caagctgcgc accccgcagg
     2041 actacgtcat cgccgccgtc cgcagcctcg acctcccccc cgacaccgtc ccgatgctcg
     2101 gcattctcgc cggcctcggc cagccggtgt ggaccgcgcc ggcgccgaac ggctggccgg
     2161 accgcgccgc cgactgggcc gcgcccgagg cgatgctgcg ccggatcgac tgggccagcg
     2221 gcttcgccgg acgtatcggc acacgcgacg tcgacgacat cgccgaaatc accctcggcc
     2281 cgctcctcgg ccgcgacacg cgtgaggcca tccgccatgc cggatcacgc cgcgacgcga
     2341 tgacgcttct gctgaccagt cccgaattcc aaaggcgctg acccatggcc ctgctttccc
     2401 gccccctttc ccgccgccac accttgctgg gccttgccac ctgcgcgagc cttggccgaa
     2461 cccatctggc aatggccgcc gcacccaccg aacgccggct ggtcgtcgtc atcctacgcg
     2521 gtgcactgga cggaatggcc acggtggtgc cctacggcga cccggccctg gccggcctgc
     2581 gcggcgccat cctgccgcca ccgccgaacc agcctgacgg tctgctcgac ctgggcgggt
     2641 tctacggcct gcatcccgcg ctgacgggtc tatatgaaat gtacaaggca ggtcaggcga
     2701 ccctggttca tgccgtggcc ggctgctacc gcgtgcgcag ccatttcgaa gcgcaggact
     2761 acctcgaatc cggcgccgac caccggatga ccagcggctg gctcaatcgc acggtcgccg
     2821 gactgccggg cgcccggtcc ggaggccctg agggtgacgc tgtggccatc ggcgtgtcgg
     2881 tcccgctgct gctgcgcggc ccggcccagg tggcgaactg ggcaccgcat ggctttgccc
     2941 agccggatgc cgcgctgtac ggccgcatcg ccgcgctgaa tgcgcatgat ccggtcaccg
     3001 gccccgccat cgccgagg
//
LOCUS       DASSZH010000562         2253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1121454, whole genome shotgun sequence.
ACCESSION   DASSZH010000562 DASSZH010000000
VERSION     DASSZH010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121454"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(275..1900)
                     /locus_tag="VFN42_12205"
     CDS             complement(275..1900)
                     /locus_tag="VFN42_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HET8997420.1"
                     /translation="MSIFEQDLERRAANYVPLSPVSFLLRSARIYGPRVAVIHGARRY
                     TYAQFLERARRLASALARAGVGKGDTVAIMAPNIPEMLEAHYAVPMLGAVLCAINIRL
                     DSGAVAFILQHSDSKLVIADREFGSVVQRALPQVGRRLLTVEIDDPEGPGGETLGGAI
                     YEDFIASGDPEFPVRLPDDEWQAIGLNYTSGTTGNPKGVVIHHRGAYLNACANALTFG
                     LSPRSVYLWTLPMFHCNGWTYTWAVTLAGGTHVCLRRVDPADIFSAIAEHGVTHMCGA
                     PVVLSMLIHAPEQVRRRPAQTVQIATGGAAPPSTVIAQMEALGFAVTHLYGLTETYGP
                     STVCLWQPGLEDLPIEQKAAFMARQGVNTPMIEAAAVLDPATMQPVPPDGATIGELML
                     RGNTVMKGYLKNPSASEEAFVGGWFHTGDLAVMHPDGYMEMKDRSKDIIISGGENISS
                     LEVEEVLYKHPAVMEAAVVARPDARWGETPQAFVTLKPGADAVGEAEIIAWCRTHMAH
                     YKCPRFVSFGPLPKTSTGKIQKFELRERARTAG"
BASE COUNT          383 a          813 c          675 g          382 t
ORIGIN      
        1 taaccacgac gcaccggccg gcttgtacca cctcgtcatg gtcggcctcc gccgatcatg
       61 acggaacgga gcagcgtgcc gcccgccaaa ccaaggagcc tccggcccga aatgtcctct
      121 tgacccactg cccgccatgc cgcgtcgggg cgaagcctcg ctatccacga ctggctggcg
      181 tgggctcgaa ctgtccggga ctggcttgcg gacccacgaa tctttctcgg caattcatat
      241 atggcgtgcc tgcgtccgcc ccgccgcggt acagctatcc cgccgtccgc gcccgttcgc
      301 gcaactcgaa cttctggatc ttccccgtcg acgtcttcgg caacggcccg aaagacacga
      361 accgcgggca tttgtagtgc gccatatgcg tgcggcacca ggcgatgatc tcggcttcgc
      421 caaccgcgtc cgctcccggc ttcagcgtca cgaaggcctg cggcgtctcg ccccagcgcg
      481 cgtccggccg agcgaccacc gcggcttcca tgaccgccgg gtgcttgtag agcacctcct
      541 ccacttccag agacgaaatg ttctcgccac cggaaatgat aatgtccttc gagcggtcct
      601 tcatctccat atagccgtcg ggatgcatca ccgcgaggtc gccggtgtgg aaccagccac
      661 cgacaaacgc ctcctccgag gccgacgggt tcttcagata gcccttcatc accgtgttgc
      721 cgcgcaacat cagctcgccg atcgtcgcgc cgtccggcgg aaccggctgc atcgtcgccg
      781 ggtccagcac cgccgccgcc tcgatcatcg gcgtgttcac gccctgccgc gccatgaagg
      841 cggccttctg ttcgatcggc aggtcctcca gccccggctg ccacaggcac accgtcgacg
      901 gaccgtaggt ttccgtcagc ccgtagagat gcgtgacggc aaaacccagc gcctccatct
      961 gcgcgatcac cgtcgatggc ggcgccgcgc cgccggtggc gatttgcacc gtctgtgccg
     1021 gacgccggcg cacctgctcc ggcgcgtgga tcagcatcga cagcaccacc ggcgcgccgc
     1081 acatatgcgt cacgccgtgt tcggcgatgg cgctgaagat gtccgccgga tcgacccgcc
     1141 gcaggcagac atgcgtgccg cccgccagcg tgaccgccca ggtgtaggtc cagccgttgc
     1201 agtggaacat cggcagcgtc cagagataca cgctgcgcgg cgacaggccg aaggtcagcg
     1261 cattggcaca ggcgttcaga taggcgccgc gatggtggat caccacgccc ttcggattgc
     1321 cggtggttcc cgacgtgtag ttcagcccga tcgcctgcca ttcatcatcc ggcaaccgca
     1381 ccgggaactc cggatcgccg ctggcgataa agtcttcata gatcgccccg ccgagtgttt
     1441 cgccgcccgg cccttccgga tcgtcaatct ccaccgtcag cagccgccgg cccacctgcg
     1501 gcagcgcgcg ctgcaccacc gacccgaatt cgcggtccgc gatcaccagc ttgctgtccg
     1561 aatgctgcag gatgaacgcc accgcgcccg agtccagccg gatgttgatc gcgcacagca
     1621 cggccccaag catcggcacg gcataatgtg cctccagcat ttccgggatg ttgggcgcca
     1681 tgatcgcgac cgtgtcgccc ttgcccaccc ccgcgcgtgc cagcgccgac gcaagccgcc
     1741 gcgcgcgttc caggaattgc gcgtaggtgt agcggcgcgc accgtggatc accgccacgc
     1801 gtgggccata gatgcgcgcg gaccgcagca ggaaggagac cggcgacagc ggtacgtagt
     1861 tcgccgcccg gcgttccagg tcctgctcga aaattgacat ggacgtcctc tgcctcgcgt
     1921 tattccgctg cgagcctaat gctgtgctcc cgccttggcg acagcccgcc ggaaggcggt
     1981 gtcctggccg gcggcagcga aatccgggag agcttgccgc aatgcggcac gggcataaag
     2041 ccgcaaccgg ccaaatcgta gcgaagttgc aatttgaccg atggcatggc gcctcgcccc
     2101 gtgaccagga aagcgaaccg atgtccttgc cgctccggaa gccgcccgcc tcccttctgc
     2161 gcgtgtcgga caagcttctt cagaccttcg aatatgcctg cgtcatgcgg gatttcgaag
     2221 tggcggagca attgctggtc atcctggaac aga
//
LOCUS       DASSZH010000563         2702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_81619, whole genome shotgun sequence.
ACCESSION   DASSZH010000563 DASSZH010000000
VERSION     DASSZH010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81619"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VFN42_12210"
     CDS             complement(<1..729)
                     /locus_tag="VFN42_12210"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8997421.1"
                     /translation="MQSTAPIRTDLVLLGAGHAHVEVLRRFARQPEPGVRLTLVAREP
                     ETPYSGMLPGLIRGDYDPAQAHIDLAPLASAAGARLILAEATGLDLQARQVAIAGRPP
                     LAYDLLSIDVGGVPALPPGSDGVPVKPIGGLRARLIELAERLPPGGRVAVIGGGPAGC
                     ELAIALMRGLRGRARIALVSETAEPVAQAPAHARRVVRAALVDCGVELISGVRALGVA
                     AGRLHLSDGSAVEVAEALWATGAVG"
     gene            851..1147
                     /locus_tag="VFN42_12215"
     CDS             851..1147
                     /locus_tag="VFN42_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET8997422.1"
                     /translation="MNRDQVVSLLRQHEPEFHEVGIGALYLFGSVARDEARDASDVDV
                     FVDLARPQGFTLFDLLGVQVRLQAILGTRVDLMTRQGIHPRRGKRIEADAVRVF"
     gene            1204..1518
                     /locus_tag="VFN42_12220"
     CDS             1204..1518
                     /locus_tag="VFN42_12220"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HET8997423.1"
                     /translation="MERQTRDLSEAEFLGNVDLEDATAYRLLAIGEAAKGLDDATRAR
                     HPDIPWNQVVAMRNILAHEYFVRESRIIWQTVKVSLPALAEACRSELVAHGWNPAGHE
                     PG"
     gene            complement(1561..2670)
                     /gene="rseP"
                     /locus_tag="VFN42_12225"
     CDS             complement(1561..2670)
                     /gene="rseP"
                     /locus_tag="VFN42_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HET8997424.1"
                     /translation="MHTVPDLARTVVSFAVVLGVLVFVHEFGHYLAARWRGVHVEVFS
                     IGFGKALASWTARSGTLWKVGWLPLGGYVKLHGQERPQDVSEEVRANWIAGQTFHEKS
                     VLSRAIIVAAGPIANFVLAAVVFTLLFATVGRPLTLPIVGSVLPHSAAAQAGLQSGDR
                     IVSIAGQPIHDFKDIQRIVTTRAGDRLSLTIERSGKQQQLSVTPQPHEVNGRDVGLLG
                     ISGSKMIYEQVSLPVAIWSGAAETWHVTAETVSGVAQMITGQRGTQDLGGPLRIAQLS
                     GEVAALGAASMLSFIAVLSVNLGLINLFPIPILDGGHLLFYLFEAVLGRPLPARAQEY
                     GFRAGLALLAGLFIFATWNDLTQFGLFHWMAGWMG"
BASE COUNT          466 a          919 c          920 g          397 t
ORIGIN      
        1 gccgacggcg ccggtggccc acaaggcttc cgccacttcg accgcgctgc cgtcggacag
       61 gtgcaaccgc ccggccgcga cgccgagggc gcgcacgccg ctgatcagct ccacgccgca
      121 atccaccagc gcggcgcgga ccacgcggcg ggcgtgggcg ggcgcctggg cgaccggctc
      181 cgcggtctcg ctgaccaagg cgatgcgggc gcgaccgcgc aggccgcgca tcagggcgat
      241 cgccagttcg catccggccg ggccgccgcc gatcaccgca acccgtccgc ccggtgggag
      301 acgctctgcg agctcgatca gccgcgcgcg cagcccgccg atcggcttca ccggaacccc
      361 atcgctgccc ggcgggaggg cgggcacgcc gccgacatcg atcgacagca ggtcataggc
      421 cagcggcggg cggccggcga tggcaacctg ccgggcctgc aggtcgaggc cggtggcctc
      481 tgccaggatc agccgcgcgc ccgcggccga agccagcggg gccagatcga tatgcgcctg
      541 ggcggggtca tagtcgccgc ggatgaggcc gggcagcatg ccggaatagg gcgtctccgg
      601 ctcgcgcgcg accagggtca gccggacgcc gggctccggt tgacgggcaa agcggcgcag
      661 cacctccaca tgggcatggc cggcgcccag caggaccagg tcggtgcgga tcggcgcggt
      721 ggattgcatg accgcaaact agcggcttcg ggccgagtcg ccggacattc ccgcgcgagg
      781 tgggtggttc aggtagcaca cggagcaatg ctggattatc ggctgccatc ggcctacatt
      841 ccggcttcgc atgaatcgcg atcaggtcgt ctccctgctg cggcaacacg aacccgaatt
      901 ccatgaagtc gggatcggtg ctttgtacct cttcggatcg gtggcccggg atgaggcgcg
      961 ggacgcatcc gatgtggatg tgtttgtcga tcttgcccgg ccgcaaggat ttactctctt
     1021 cgacctcctt ggcgtccagg tccgcctgca agccatcctg gggactcgcg ttgacctgat
     1081 gacgcgccaa ggcatccatc cgaggcgcgg gaagcgcatt gaagccgacg cggtgcgggt
     1141 attctgatgc cgggcggctc cccattggag agcctgctgg acattctgga gtagatcgaa
     1201 cgcatcgagc ggcagacacg cgacctctcc gaggcggaat tcctcggcaa cgtggatctg
     1261 gaggacgcga cggcctatcg gctactggcg ataggcgagg ccgcaaaggg tctggacgat
     1321 gccaccaggg cgcggcaccc ggacattccc tggaaccagg ttgtcgccat gcgcaatatc
     1381 ctggcgcacg agtatttcgt ccgggaaagc cggatcatct ggcagaccgt caaggtgagc
     1441 ctaccggctt tggcggaagc ctgccgtagc gaactcgttg cacacggctg gaatcccgcc
     1501 gggcacgagc ccggctaaat ccgactgatt cccctcagcg ccggaacgcc ggccacgcgc
     1561 ctagcccatc catccggcca tccagtggaa caggccgaac tgggtcaggt cgttccaggt
     1621 ggcgaaaatg aacagcccgg ccagcaacgc cagtccggcg cggaagccat attcctgtgc
     1681 gcgcgccggc agcgggcggc ccagcaccgc ctcgaacaga tagaacagca agtgcccgcc
     1741 atccaggatt gggatcggga acaggttgat cagccccaga tttaccgaca gcacggcaat
     1801 gaaggacagc atgctggccg cacccagtgc agccacttcg cccgacaact gtgcgatgcg
     1861 cagtgggccg ccgaggtcct gcgtgccgcg ctgcccggtg atcatctgcg ccacgccgct
     1921 gaccgtctcg gccgtgacgt gccaggtctc cgctgcgccg ctccatatcg cgacgggcag
     1981 cgacacctgc tcgtagatca tcttgctgcc gctgatgccc agcaggccga catccctgcc
     2041 gttcacctca tgcggctgcg gcgtgacgga gagctgctgc tgcttgccgc tgcgctcgat
     2101 ggtcaggctg agcctgtccc ccgcgcgggt ggtgacgatg cgctggatgt ccttgaaatc
     2161 gtggattggc tggccggcga tgctgacgat ccgatcgccg gactgcagcc ccgcctgcgc
     2221 cgccgcgcta tgcggcagga cgctgccgac gatcggcagg gtcagcggcc ggccgaccgt
     2281 ggcgaacagc agcgtgaaca ccaccgcggc cagaacgaaa ttggcgatcg ggccggcggc
     2341 aacgatgatg gcgcgcgaca gcaccgactt ctcgtggaag gtctgcccag cgatccagtt
     2401 ggcgcgcact tcctccgata cgtcctgcgg gcgctcctgg ccgtgcaact tgacgtagcc
     2461 gcccagcggc agccagccaa ccttccacag cgtgccggaa cgcgccgtcc aggaggccag
     2521 cgccttgccg aagccgatgg agaacacttc cacatggacg ccgcgccagc gggcagcgag
     2581 gtagtgaccg aactcgtgga cgaagaccag cacgcccagc accaccgcaa agctcacgac
     2641 ggtgcgggcc agatcgggga cggtgtggat cacggtgagc ggttgcttct gctaagaacg
     2701 gg
//
LOCUS       DASSZH010000564         5109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_64864, whole genome shotgun sequence.
ACCESSION   DASSZH010000564 DASSZH010000000
VERSION     DASSZH010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64864"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..910)
                     /gene="yghU"
                     /locus_tag="VFN42_12230"
     CDS             complement(38..910)
                     /gene="yghU"
                     /locus_tag="VFN42_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HET8997425.1"
                     /translation="MSDAAEYTPPKVWTPKPALGRFANINRPVSGATHQKELPVGKHP
                     LQLYSQGTPNGVKVTVMLEELLAAGHKGAEYDAWLIRINEGDQFGSGFVAINPNSKIP
                     ALVDRSGPQPIRVFETAAILVHLAEKFGALLPTEPAKRAECFSWLFFQMGSAPYLGGG
                     FGHFYAYAPVKIEYAIDRFTMEAKRLLDVMDKRLGESEYVAGSDYTIADIACWPWYAG
                     LVKFNQYGATEFLSVHEYKNVVRWADAIWARPAVKRGRMVNRTQGDPSEQLWERHDAS
                     DFETKTQDKLAAAQ"
     gene            complement(982..2133)
                     /gene="lpxB"
                     /locus_tag="VFN42_12235"
     CDS             complement(982..2133)
                     /gene="lpxB"
                     /locus_tag="VFN42_12235"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010513538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HET8997426.1"
                     /translation="MPLIYLIAGEASGDVLGSRLMQAIAAARPDARFAGVGGAAMTAL
                     GLRSLFPMQELALMGLVEVLPKLRRLRARLTETAADIAAQRPDVVVTIDSPGFTLRVL
                     RAIAPLGIPRAHYVAPQVWAWREKRVRHYPGLWERLLCLLPFEPAFFARHGLAASFVG
                     HPVLESGADRGDAARFRARHGIPPESRLVTLMPGSRRTEVARLLPILGETLRLVSAAV
                     PGLRPVVPLAGPVAEAVRHGTSAWPVPPLLVESPQDKYDAFAASSAALTKSGTSTLEL
                     ALAGVPMLVTYRVNPLSAALVRRTVKVRYASLLNLLADREVVPELLQQDCRPERLAPV
                     LRSLLLDDAAAEAQRAGFAATLALLRPPEGLPSQAAAASVLELLDRPAR"
     gene            2318..2719
                     /locus_tag="VFN42_12240"
     CDS             2318..2719
                     /locus_tag="VFN42_12240"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HET8997427.1"
                     /translation="MFSLTVCDHIMIAHSFRGAEFGPAQRLHGATYAVEAEFRAPKLD
                     ASQLLVDIGLAKAELRRVLDTVDYRNLDDLPALQGTNTTTEYMAFHLHGLLAAACRDG
                     RLGEGGRAVSGLKLLLRESPNAWAAYEGPIA"
     gene            2719..3753
                     /locus_tag="VFN42_12245"
     CDS             2719..3753
                     /locus_tag="VFN42_12245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET8997428.1"
                     /translation="MHAALLVPAPFDQVSGGYAYDRRMMQGLRATGHRVDVVELSGRY
                     PLADNAARDAACAAWDRLPEGTRPIIDGLALPAFAGMQDALAARGCIGLIHHPTALET
                     GFSAEDREALHAIEQRLFQHLHRIVVTSDPTAERLAKEFGVDPARIVTVVPGTDDAPR
                     SQGSGGPGCAILSVGTLVPRKGHDVLLRALARLFDLDWHLTIAGSPHRHPAHAQALVT
                     LAEELRIADRVRFAGEVVDEALEALWQGADLFALATWFEGYGMAVAEALKRGLPVAVT
                     SGGAAAALVQAESGVVCQPGDLDQFAKALRRLIFDTELRREMAEVAWQSGQRLPNWDQ
                     QAQSFAAAIR"
     gene            3684..4814
                     /locus_tag="VFN42_12250"
     CDS             3684..4814
                     /locus_tag="VFN42_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET8997429.1"
                     /translation="MAKRPKTAELGPAGAKFRSGDPLNGGDLWSLAEALSAVLAEATE
                     ELPPDPTAELAKRLREVTSPFLQRRRGMRSGQAVEQPFDAEWLALREPFDARARNRNL
                     AHRFAASLPERVTLLELGAGTGSLFRWLAPIIARPQQWLCFDEDENHLQRGVRLTASW
                     GRRLGYTVEPGEDETSLTLHTPHGPWSMQGVGCDLEDPPVFLPLDDVDAVVCSALLDL
                     FSEQWLTELVFSLGRVPFYAAMTVTGGGWSNHHDPDDALVREGFRRNQTGNGPRSDGL
                     GPEAAFAAEWLARQVGLHYEWRRSDWTIGPRDRAMLRRVLAFHAGGARNALPQHRARI
                     DAWEARRNRDVDAGRLAMRIAHRDLLISTPEKGPRHAARRRR"
     gene            4792..>5109
                     /locus_tag="VFN42_12255"
     CDS             4792..>5109
                     /locus_tag="VFN42_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HET8997430.1"
                     /translation="MLLGAVADDFTGATDLCSMLVRGGMRTVQLIGVPDASDPPPDAD
                     AVVVALKSRTAPVRQAVDDSLAALDWLRKAGAKQFFFKYCSTFDSTDQGNIGPVADAM
                     IEAL"
BASE COUNT          770 a         1806 c         1766 g          767 t
ORIGIN      
        1 ccgagcttcc acttggccca ccaatgaccc aggcccgcta ctgcgccgcg gcgagcttgt
       61 cctgtgtctt cgtctcgaag tcgctggcgt cgtgccgctc ccaaagctgc tcggatggat
      121 cgccctgcgt gcggttcacc atccgccccc gcttcaccgc ggggcgagcc cagatcgcgt
      181 ccgcccaacg caccacgttc ttgtattcgt gcaccgacaa gaactcggtc gcgccgtact
      241 ggttgaactt caccagcccg gcataccagg gccagcacgc aatatcggcg atggtgtagt
      301 cgctgcccgc cacgtactcg ctctcgccca gccgcttgtc cattacatcc agcagccgct
      361 tggcttccat ggtgaagcgg tcgatcgcgt attcgatctt caccggcgca taggcgtaga
      421 agtggccgaa cccgccaccg agatacggcg cgctgcccat ctggaagaac agccaggaaa
      481 agcactccgc ccgcttcgcc ggctcggtcg gcagcagggc gccgaacttc tccgccagat
      541 gcaccaggat ggcggcggtc tcgaataccc ggatcggctg tggcccgctg cggtccacca
      601 gcgcgggaat cttcgagttc ggattgatcg ccacgaagcc gctgccgaac tggtcgccct
      661 cgttgatgcg gatcagccag gcgtcgtatt cggcgccttt gtggccggcg gccagcagtt
      721 cctccagcat cacggtgacc ttcacgccgt tcggcgtgcc ttgcgagtag agctgcagcg
      781 ggtgcttgcc gaccggcagt tccttctgat gcgtggcgcc ggaaaccggg cgattgatgt
      841 tggcgaagcg acccagcgcc ggcttgggcg tccagacctt gggcggcgtg tactcggctg
      901 cgtcggacat gatggaaccc tccgggctga tgtttgatcg caacgtggcc cggctatcgg
      961 gatggcgcaa cccggtgggc gtcagcgcgc cggcctgtcc agcagttcca gcaccgacgc
     1021 ggcggcggcc tgcgacggca agccctcggg cggtcgcagc aacgccagag tggcggcaaa
     1081 gccggcccgt tgcgcctccg ccgccgcatc gtccagcagc aggctgcgca gcaccggcgc
     1141 cagccgctcc ggccggcagt cctgctgcag cagttccggc accacctccc tgtcggccag
     1201 caggttcagc agcgaagcgt agcgcacctt cactgttcgc cgcaccagag ccgcggagag
     1261 cgggttgacc cggtaggtca ccagcatcgg cacgccggcc agcgccagct ccagcgtgga
     1321 ggtgccggac ttggtcagcg ccgccgacga ggcggcgaag gcgtcgtact tgtcctgcgg
     1381 cgactccacc agcagcggcg gcaccggcca tgcgctggtg ccgtgccgca ccgcctcggc
     1441 caccggtccg gccagcggca ccacgggccg cagaccgggg accgccgcgg acaccagccg
     1501 cagcgtttcg cccaggatcg gcagcaggcg cgcgacctcg gtgcgccggc tgcccggcat
     1561 cagcgtgacc agccgggact ccggcggaat gccgtggcgg gcgcggaagc gcgcggcgtc
     1621 gccgcgatcg gcgccgctct ccagcaccgg atggccgacg aagctggcgg ccaatccgtg
     1681 ccgagcgaag aacgccggct cgaacggcag caggcagagc agccgttccc agagcccggg
     1741 gtagtggcgc acccgcttct cccgccaggc ccagacctgc ggggcaacgt agtgcgcgcg
     1801 cgggatgccg agcggcgcga tggctcgcag cacgcgcagg gtgaagccgg gcgaatcgat
     1861 ggtgaccacc acgtccggcc gctgcgccgc gatatccgcg gcggtctccg tcaggcgggc
     1921 gcgcagccgg cgcagcttgg gcagcacctc caccagcccc atcagcgcca gttcctgcat
     1981 cgggaacagg ctgcgcaggc ccagcgcggt cattgccgcg ccgccgacgc cggcgaaccg
     2041 ggcatccgga cgcgcggcgg cgatggcctg catcaggcgg ctgccaagaa cgtcgccgct
     2101 ggcttcgccg gcgatcaggt agatcagggg catgggcgac aggattcgca aaatgtgccg
     2161 gacgttcgga gccggttcgc catctgaccg taccccgtcg tggccggcga aacgatgccg
     2221 tatgaccggc ggaaggtact aggtgctggc gcgccgttcg gcaagcgccg ggggccgggc
     2281 gagacagcgg ccagcttccg gtgtagctgt agcacccatg ttcagcctga ccgtctgcga
     2341 ccacatcatg atcgcgcaca gtttccgcgg cgccgagttc ggaccggcgc agcggctgca
     2401 cggcgccacc tacgcggtgg aagcggagtt ccgcgcgccc aaattagacg cctcccagtt
     2461 gctggtggat atcggcctgg ccaaggccga gctgcgccgc gtgctggata cggtggacta
     2521 ccgcaacctg gacgatctcc cggcgctgca gggcaccaac accaccaccg aatacatggc
     2581 tttccatctc cacggcctgc tcgcggccgc gtgccgcgat gggcggctgg gggagggcgg
     2641 acgcgcggtc agcggactga agctgctgct gcgggaaagc ccgaatgcct gggccgccta
     2701 tgaaggaccg atcgcctgat gcacgccgcg ctgctggtac cggccccgtt cgaccaggtg
     2761 tcgggcggct atgcctatga ccgccgcatg atgcagggcc tgcgcgcgac cggtcaccgg
     2821 gtcgatgtgg tggaactgtc cggccgctac ccgctggccg ataacgccgc ccgcgacgcg
     2881 gcgtgtgccg cctgggaccg gctgccggag gggacccggc cgatcatcga cggcctggca
     2941 ttgcctgcct tcgccggcat gcaggacgcg ctcgccgcgc gcggctgcat cggcctgatc
     3001 catcatccca ccgcgctgga gaccggcttc tccgccgagg accgcgaggc gctgcacgcc
     3061 atcgagcagc gcctgttcca gcatctgcac cgcatcgtgg tgaccagcga cccaaccgcg
     3121 gaacgcctgg ccaaggaatt cggcgtcgat ccggcccgca tcgtcacggt ggtgcccggc
     3181 accgacgacg cgccgcgcag ccagggttcc ggcggtccgg gctgcgccat cctgtcggtc
     3241 ggcacgctgg tgccccgcaa gggtcacgac gtgctgctgc gcgcgctcgc ccgcctgttc
     3301 gacctggact ggcatctgac catcgccggt tcgccgcatc gccatccggc gcatgcccag
     3361 gccctggtaa ccctggccga ggagctgcgc atcgccgacc gtgtgcgctt cgccggcgag
     3421 gtggtggatg aggcgctgga agcgctgtgg cagggcgccg acctgttcgc actggccacc
     3481 tggttcgaag gctacggcat ggcggtggcc gaggcgctga agcgcggcct gcctgtcgcg
     3541 gtgacctccg gcggtgccgc ggcggcgctg gtgcaggcgg agtccggcgt ggtttgccag
     3601 cccggcgacc tggatcagtt tgccaaggcg ctgcgccggc tgatcttcga taccgaattg
     3661 cgccgcgaaa tggcggaggt ggcgtggcaa agcggccaaa gactgccgaa ctgggaccag
     3721 caggcgcaaa gtttcgcagc ggcgatccgc tgaacggcgg cgatctctgg tcgctcgcgg
     3781 aagccttgag cgcagtgctg gcggaggcga ccgaagaact gcctccggat cccacggccg
     3841 agctggcaaa gcggctgcgg gaagtcacgt cgcccttcct gcagcgccgg cgcggcatgc
     3901 ggtccgggca ggccgtcgaa cagccgttcg atgccgaatg gctggcgttg cgcgagccgt
     3961 tcgacgcccg ggcgcgcaac cgcaacctgg cgcaccgctt cgccgcatcg ctgcccgaac
     4021 gggtcacgct gctggaactc ggcgccggca ccggaagcct gttccgctgg ctcgctccca
     4081 tcatcgcccg gccgcagcaa tggctgtgct tcgacgagga tgagaaccac ctgcaacgcg
     4141 gcgtgcgcct caccgcgtct tgggggcggc gcctgggcta caccgtggag ccaggcgagg
     4201 acgagacgag cctgacgttg cacacgccgc acggcccctg gagcatgcag ggcgtgggtt
     4261 gcgacctgga ggatccgccg gttttcctgc cgctcgatga tgtggacgcg gtggtttgca
     4321 gcgcgctgct ggacctgttt tccgaacaat ggttgacgga actggtgttc tcgctgggcc
     4381 gggtgccgtt ctatgcggcg atgacggtga ccggcggggg gtggagcaac catcacgacc
     4441 ccgacgacgc gctggtgcgt gaaggcttcc gccgcaacca gactggcaac ggcccccgca
     4501 gcgatgggct cggcccggaa gcggccttcg ccgcggagtg gctggcccgg caggttgggc
     4561 tccattacga gtggcgccgt tccgactgga ccatcggccc gcgcgatcgc gccatgctgc
     4621 gccgcgttct cgccttccat gccggcggcg cgcgcaacgc cctgccgcag catcgcgccc
     4681 gcatcgatgc ctgggaagcg cggcggaacc gcgatgtcga tgccggccgg cttgccatgc
     4741 gcatcgccca ccgcgacctt ctgatctcca ccccagaaaa aggaccacgc catgctgctc
     4801 ggcgccgtcg ctgacgactt caccggggcg accgacctct gctccatgct ggtgcgcggc
     4861 ggcatgcgca ccgtgcagtt gatcggcgtg ccggacgcct ccgacccgcc gccggatgcc
     4921 gacgccgtgg tggtggcact gaagtcgcgc accgcaccgg tgcgccaggc ggtggacgac
     4981 agcctcgccg cgctggactg gctgcgcaaa gccggcgcga agcagttctt cttcaaatac
     5041 tgctcgacct tcgacagcac cgaccagggc aatatcggcc cggtcgccga cgccatgatc
     5101 gaggcgctc
//
LOCUS       DASSZH010000565         2676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_865825, whole genome shotgun sequence.
ACCESSION   DASSZH010000565 DASSZH010000000
VERSION     DASSZH010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865825"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VFN42_12260"
     CDS             complement(<1..315)
                     /locus_tag="VFN42_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HET8997431.1"
                     /translation="MLTPLAWLLGLALPACGMAGAHGVPSAAPDFSHLERPSSPNTAL
                     AAPAGYVPAPDIVTPVYKVPATRLFAVVQQVAAEQSATYALAEDKPALREGWVARSFV
                     WNF"
     gene            complement(322..2013)
                     /locus_tag="VFN42_12265"
     CDS             complement(322..2013)
                     /locus_tag="VFN42_12265"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HET8997432.1"
                     /translation="MAGKLRTDPARFEVVKNALFAAAEEMKIVLAKTAYSPLLKVAGD
                     YSCGIFDVSGNMVAQGPDLPIHLGSMPDAVRAVVAAFPDVAPGDVFIHNDPYQGGSHL
                     PDVNVVAPAFHANRLLGFGCVRAHWPDIGSATPGSYGAVTEIYGEGLRLPPVRLYRDG
                     KPDAAVEAIIFANVRTPAERLGDLRAQVAANWRAAQRLSELAAKYGADTLLQIMQEVL
                     DYSETMMRAALRALPDGEAEFTDVFDGDGVIADGETEDATFTVKLRITKRGDSIVADF
                     AGSDPAVAGPMNAPLTVTASGVFCALKMIADPKSLIPPNSGCWRPVTVTAPPGSVVHA
                     QHPSPVVYANHEMSHRIADMVMGAMFAITPRTVMSGSQGTSAVITFGGVDYRSGDRFV
                     SYESLKGGFGARPVKDGINAVASTVSNMSNTPIEILEMSFPLRVEEYALVPDSGGAGK
                     YRGGLGVRRVWRVLERDAHASVCCERTVTPPFGLDGAQAGGAARLTLVPPHGNARRLN
                     SKGGFIAPAGALVVVEAPGSGGYGAPPERDGTALAADLADGYVTPAAARRDYGRG"
     gene            complement(2017..>2676)
                     /locus_tag="VFN42_12270"
     CDS             complement(2017..>2676)
                     /locus_tag="VFN42_12270"
                     /inference="COORDINATES: protein motif:HMM:NF039581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HET8997433.1"
                     /translation="QIPEIIIPPAPGAFSALGLVASDLKRDYSRTLYADLAQVEPHRV
                     ADGFAGMEAAGGAMLEQARVPAEQRALLRFADVRYRRQAYELTVPVAEGPVTRDTLDA
                     LAEAFHARHAQTYGHANRAETVQLVNLRVTALGRLPGLQLTQRGDPASARVRTRDVWF
                     PHAGFVTTRVHWRDGLSPGSVLAGPLVIDALDSTTVVPPGWQASVDTWGCIRIRRRSE
                     Y"
BASE COUNT          430 a          971 c          871 g          404 t
ORIGIN      
        1 gaaattccac acgaagctgc gcgccaccca gccttcccgc agcgccggct tgtcctcggc
       61 cagcgcatag gtcgcgcttt gctccgccgc gacctgctgc accacggcga acaggcgtgt
      121 ggccggcacc ttgtaaaccg gggtgacgat atccggtgcc ggcacatatc cagccggcgc
      181 cgccagtgcg gtgttcgggc tgctcggccg ctccagatgg gagaaatccg gcgcggccga
      241 gggaacgccg tgcgcgcctg ccatcccgca ggccggcaga gccagcccca gcagccaggc
      301 cagaggcgtc agcatccgcg atcaaccacg gccgtagtcg cgccgcgccg cagccggtgt
      361 gacgtagccg tccgccagat ccgccgccag tgccgtgcca tcccgttccg gcggggcgcc
      421 atagccgccg gaacctggag cttcgaccac caccagcgcg ccggccggtg cgatgaagcc
      481 gcccttgctg ttcagccgtc gcgcattgcc atgcggcggc accagcgtca gccgcgccgc
      541 accgccggcc tgcgcgccgt ccagaccgaa aggcggcgtc accgtacgct cgcagcaaac
      601 ggacgcgtgt gcgtcgcgtt ccagcacgcg ccacacccgc cgtacgccca agccgccgcg
      661 gtatttgccc gcgccaccgc tgtccggcac cagcgcgtat tcctccaccc gcagcgggaa
      721 actcatctcc aggatttcga tcggcgtgtt gctcatgttg gacacggtgc tggccacggc
      781 gttgatcccg tccttgaccg gccgcgcgcc gaacccgccc ttcagcgatt cgtagctgac
      841 gaagcgatcg ccgctgcggt aatccacccc gccgaaggtg atcaccgcgg acgtcccctg
      901 cgatcccgac atcaccgtgc gcggcgtgat ggcgaacatc gcgcccatca ccatgtcggc
      961 gatgcggtgc gacatctcgt ggttggcata gaccaccggc gacgggtgct gcgcatgcac
     1021 cacggaaccc ggtggtgcgg tcaccgtcac cgggcgccag cagccggaat tcggcgggat
     1081 caggcttttc ggatcggcga tcatcttcag cgcgcagaac acgccggacg cggtcaccgt
     1141 cagcggcgcg ttcatcggcc cagccacggc gggatccgag ccggcgaaat ccgcgacgat
     1201 gctgtcgccg cgcttggtga tgcgcaactt gacggtgaag gtggcgtcct ccgtctcgcc
     1261 atcggcaatg acgccgtcgc cgtcgaacac gtcggtgaac tctgcctcgc catcgggcag
     1321 agcacgcaac gcggcgcgca tcatggtttc cgaatagtcc agcacttcct gcatgatctg
     1381 cagcagcgtg tcggcgccat acttcgccgc cagctccgac agtcgctggg cggcgcgcca
     1441 gttcgcggcg acctgggcgc gcaaatcccc tagccgttcc gcgggcgtgc gcacattggc
     1501 gaagatgatg gcttccaccg ccgcgtccgg cttgccgtcg cgatacagcc gaaccggcgg
     1561 caggcgcagg ccctcgccat agatttcggt caccgcgccg tagctgcccg gcgtggcact
     1621 gccgatatcc ggccagtgcg cccgcacgca gccgaagccc agcaggcggt tggcgtggaa
     1681 ggcgggcgcg acgacgttga cgtcgggcag atgcgagccg ccctggtagg gatcgttgtg
     1741 gatgaacacg tcgcccggcg cgacatccgg gaaggcggcg accaccgcgc gcaccgcgtc
     1801 tggcatggag cccaggtgga tcggcagatc ggggccctgc gccaccatgt tgccgctgac
     1861 atcgaagatg ccgcaggaat agtcgcccgc cactttcagc agcggggaat acgccgtctt
     1921 ggccaggacg atcttcattt cctcggccgc ggcgaacagc gcgttcttca ccacctcgaa
     1981 gcgggcggga tcggtgcgaa gcttaccggc cattccctag tactccgatc tgcgtcgaat
     2041 gcggatgcag ccccatgtat ccaccgaggc ctgccacccc ggcggcacca cggtggtgga
     2101 atccagcgcg tcgatcacca gcggcccggc aagcacgctg ccgggcgaca atccgtcgcg
     2161 ccagtgcaca cgtgtggtga cgaagccggc gtgcgggaac cacacatccc gcgtgcgtac
     2221 ccgcgcgctg gcgggatcgc cacgctgcgt cagttgcaat cccggcagcc ggcccaacgc
     2281 ggtgacccgc agattcacca actgcaccgt ctccgcccgg ttggcatggc cgtaggtctg
     2341 tgcgtgccgt gcgtggaagg cttccgccag cgcgtccagt gtgtcacgcg tcaccggacc
     2401 ttccgccacc ggcacagtca gttcgtaggc ctgccggcga tagcgcacgt cggcgaagcg
     2461 cagcagcgcg cgttgttcgg cgggcacgcg ggcctgttcc agcatcgccc cgcccgctgc
     2521 ttccatcccg gcgaaaccat ccgccacgcg atgcggttcc acctgcgcca ggtcagcgta
     2581 caacgtgcgg gagtagtcgc gcttcagatc gctcgccacc agccccagcg cggagaaggc
     2641 accgggggcg ggcgggatga tgatctcggg aatctg
//
LOCUS       DASSZH010000566         3067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_627409, whole genome shotgun sequence.
ACCESSION   DASSZH010000566 DASSZH010000000
VERSION     DASSZH010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627409"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VFN42_12275"
     CDS             <1..731
                     /locus_tag="VFN42_12275"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET8997434.1"
                     /translation="AWWWGVLAGIAVAVWPLVVALPRRRWRHAVVHHAMRGFFRLTRI
                     PLTLAAEAPLPDGGAILAVNHASYLDGAVLAALCPGVLTFTAKQELAGQWVAGPFLRR
                     LGTVFLRRTEAAGGVADAAAALPVLRGGAHLVWFPEGTLTRMPGLLGFHLGAFLVAAE
                     AGVPVVPVTISGTRAVLRGGQWFPRPGAIQVRIGRALMPDGRDFAAAVRLRDRTRAAI
                     LAQCGEPDLSHEHPSPGVRTLPGA"
     gene            934..2550
                     /locus_tag="VFN42_12280"
     CDS             934..2550
                     /locus_tag="VFN42_12280"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET8997435.1"
                     /translation="MNDTAPDFDAVVIGAGFSGMYMLKSLRDRLGLKVRVYEAGDTVG
                     GTWYWNRYPGARCDSDAYIYCFTWDKQLLQEWEWSERYPEQPEILRYLEHVARRHDLN
                     RDIQFNTRVTGGEFDEDANLWRIHTDKSETVTARFLISAVGTLSETNLPPFKGLERFK
                     GKWYHTSRFPQEGVDFTARRVAVVGTGATAVQAIPEIAQQAKHLTVFQRTANYCVPAR
                     NGKVDPERVKARKADYDGIVHRIRDSFFGQEHYFIPKSALEASPEEREREFDRMWDSG
                     GFAFWLANYQDMFFSQDANDLCADYIKRKIRRTVKDPAVAEKLIPKGYAYGTKRQPLD
                     TNYYETFNKDNVLLVDAAADGPIEEITEKGIRAGGKEYEFDIIVFATGFDALTGPLKA
                     LHLKGRGGRTLDQEWASGPRTYLGIAVAGFPNLFTITGPQSPSVLTNMPVAIEQHVEW
                     ITECIDAMHQNGQTVVEATPDAQEKWVDHVNEIVSMTLMTGANSWYMSANIPGKPRAF
                     LPYLGPQGVGGYRRTCDEVAAKGYEGFALA"
     gene            2618..>3067
                     /locus_tag="VFN42_12285"
     CDS             2618..>3067
                     /locus_tag="VFN42_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997436.1"
                     /translation="MISNSYYTQDVHGPYELHDIGNLELEEGGTIRGCQLACATYGKL
                     NAAGDNAILIPTWYSGTHKIIEQVYIGKGRALDPEKYFILVVNQIGSGLSTSPHNTPS
                     PGGMAHFPRVRIGDDVRAQHRLLTEKLGIRSLALVVGGSMGAQQTYEW"
BASE COUNT          537 a         1028 c         1034 g          468 t
ORIGIN      
        1 cggcgtggtg gtggggcgtg ctggcgggca tcgcggtcgc ggtctggccg ctggtggtcg
       61 cgctgccgcg gcggcgctgg cggcatgcgg tcgtgcatca cgcgatgcgg ggcttcttcc
      121 ggctgacgcg cattccgctc acgctcgcag ccgaggcgcc gctgccggac ggcggcgcga
      181 tcctcgccgt caatcacgcg agctacctgg acggcgcggt gctggcggca ctctgtcccg
      241 gcgtgctgac cttcaccgcc aagcaggaac tggccgggca atgggtggcc ggcccgttcc
      301 tgcgccggct gggcaccgtg ttcctgcgcc gcaccgaggc ggcgggcggc gtcgccgatg
      361 ccgcggcggc gctgccggtg ctgcggggcg gcgcgcatct ggtctggttt cccgaaggca
      421 cgctcacgcg catgccgggc ctgctcggct tccatctcgg cgccttcctg gtcgccgccg
      481 aggccggcgt gccggtcgtg ccggtgacca tcagcggcac gcgcgcggtg ctgcgcggcg
      541 gccagtggtt tccgcggccg ggtgcgatcc aggtgcggat cggccgggcg ctgatgccgg
      601 acggacgcga cttcgcggcg gccgtgcgcc tgcgcgaccg gacgcgggcg gcgatcctgg
      661 cgcagtgcgg cgaaccggac ctgtcccacg aacacccctc ccccggcgtg cgcaccctgc
      721 cgggcgcgta ggtccggcgc acacactcgt gaacgctggc ggcggttgcc cgcgatccgc
      781 cgaagctgcc cgcaacccct ggattcttca ctgccggacg ttcggccatc ggatgaatcg
      841 gcggacacgt cgccgtggcg gcgacgcagg tcgccttgat gaagactgac agcctgacta
      901 gcgttttcgc ccgcgggacc acgggaggaa aacatgaacg ataccgcacc ggacttcgac
      961 gccgtcgtga tcggcgccgg gttttccggc atgtacatgc tcaagtcgct gcgcgacagg
     1021 ctcgggttga aggtacgggt ctatgaggcc ggcgacaccg tcggcggcac ctggtactgg
     1081 aaccgctatc cgggcgcacg ttgcgactcc gatgcctata tctattgttt cacctgggac
     1141 aagcagttgc tgcaggaatg ggagtggtcg gaacgctatc ccgagcagcc ggaaatcctg
     1201 cgctatctgg aacacgtcgc cagacgccac gacctcaacc gcgacatcca gttcaacacc
     1261 cgcgtcaccg gcggcgaatt cgacgaggac gccaatctct ggcgtatcca cacggataag
     1321 tccgagaccg tcacggcccg tttcctgatc agtgccgtcg gcacgctgtc ggagacgaac
     1381 ctgccgccgt tcaaggggct ggagaggttc aagggcaagt ggtaccacac cagccgcttc
     1441 ccgcaggaag gggtggactt caccgccagg cgcgtcgcgg tggtcggcac gggtgcgaca
     1501 gcggtgcagg cgattccgga gattgcccag caggccaagc atctcaccgt tttccagcgc
     1561 accgccaact actgcgtgcc ggcgcgcaac ggcaaggtcg atcccgaacg ggtcaaggca
     1621 cgcaaggcgg attacgacgg catcgtccac cgcatccgtg attccttctt cggacaggaa
     1681 cattacttca tcccgaaatc cgcgctcgaa gcctcgcccg aggagcgcga gcgcgagttc
     1741 gaccgcatgt gggacagcgg cggcttcgcc ttctggctgg cgaactatca ggacatgttc
     1801 ttcagccagg acgccaacga tctgtgcgcc gactacatca agcgcaagat ccgccgcacg
     1861 gtgaaggatc ccgccgtggc cgagaagctg atcccgaagg gatacgccta cggcaccaag
     1921 cgccagccgc tcgacaccaa ctactacgag acgttcaaca aggacaacgt gctgctggtc
     1981 gatgccgcgg ccgacggccc gatcgaggag atcacggaga agggcatccg cgccggcggc
     2041 aaggagtatg agttcgacat catcgtcttc gccaccggct tcgacgccct gaccggcccg
     2101 ctgaaggcgc tgcacctgaa aggacgcggc gggcgcacgc tggatcagga atgggcgtcg
     2161 ggaccgcgca cctatctcgg catcgcggtc gccgggttcc ccaacctgtt caccatcacc
     2221 ggcccgcaaa gcccctcggt gctgacgaac atgccggtcg cgatcgagca gcacgtggag
     2281 tggatcaccg agtgcatcga cgccatgcac cagaacggcc agaccgtcgt cgaggcgacg
     2341 ccggacgcgc aggagaaatg ggttgaccac gtcaacgaga tcgtctccat gacgctgatg
     2401 accggggcga attcctggta catgagcgcc aacatcccgg gcaagccgcg cgccttcctg
     2461 ccgtatctcg gcccgcaggg cgtcggcggc tatcgcagga catgcgacga ggtggccgcc
     2521 aagggctacg aaggattcgc gctggcgtaa cgagccgggc gcgaagcggg cgggacggca
     2581 gacacgtccc gccccacgac aacaacggga ggaaaccttg atcagcaaca gctactacac
     2641 gcaggacgtg cacggcccct atgagctgca cgacatcggc aacctcgaac tggaggaagg
     2701 cggcaccatc cgcggctgcc agctggcctg cgccacctac ggcaagctca atgcggcggg
     2761 ggacaatgcg atcctgatcc cgacgtggta ttcgggcacg cacaagatca tcgagcaggt
     2821 ctatatcggc aagggccgcg cgctcgatcc cgagaaatac ttcatcctcg tggtcaacca
     2881 gatcggcagc gggctttcga catctccgca caacacgccg tcgccggggg ggatggcgca
     2941 tttcccgcgc gtgcgcatcg gcgacgacgt gcgcgcgcag caccggctgt tgacggagaa
     3001 gctcggcatt cgcagcctgg cactggtcgt cggcggctcg atgggcgcgc agcagaccta
     3061 tgaatgg
//
LOCUS       DASSZH010000567         3083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_780627, whole genome shotgun sequence.
ACCESSION   DASSZH010000567 DASSZH010000000
VERSION     DASSZH010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780627"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VFN42_12290"
     CDS             complement(<1..242)
                     /locus_tag="VFN42_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997437.1"
                     /translation="MDGADILLRHPVPRHRITVADYHRLAEAGILAAGDRVELLDGQL
                     VDMPPIGPRHALTVDALMYALIAAIADRAAVRVQQPV"
     gene            complement(388..1431)
                     /locus_tag="VFN42_12295"
     CDS             complement(388..1431)
                     /locus_tag="VFN42_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006558701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET8997438.1"
                     /translation="MFSRVLGFMSADMAIDLGTANTLVYVKGRGIVLAEPSVVAIADV
                     RGKKQVVAVGEEAKQMLGRTPGYITASRPLRDGVIADFEVAEEMIKHFIRKVHNRRGF
                     ASPLIIVCVPSGSTAVERRAIQESAESAGARRVQLIEEPMAAAIGAGLPVTEPSGSMI
                     VDIGGGTTEVAVISLGGIVYSRSVRVGGDKMDEAIISYIRRNHNLLIGESTAERIKMD
                     IGAANAPEEGDDGPYREVRGRDLMNGVPREVLVGQRQVAESLSEPVSAIVEAVKVALE
                     NTPPELAADIVDKGIVLTGGGALLYRLDQVLRDATGLAVVVAENPLQCVALGTGRALE
                     ERKRLSNVLSTMY"
     gene            1609..1821
                     /locus_tag="VFN42_12300"
     CDS             1609..1821
                     /locus_tag="VFN42_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997439.1"
                     /translation="MKRKIACGLALAMMLGLAGCGYSPGTRAVTGGLLGAGAGAGIAA
                     ATGGRPATGAAIGAGAGAVGGAVTSP"
     gene            1848..2117
                     /locus_tag="VFN42_12305"
     CDS             1848..2117
                     /locus_tag="VFN42_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HET8997440.1"
                     /translation="MSTRNRKSTGRSIATGVLLTGLVLGLSGCGYSRGDRAVSGGLIG
                     AGGGAAIGALTGNPLAGAAIGGAGGALVGAATNPHDVNLGRPVWR"
     gene            2294..>3083
                     /locus_tag="VFN42_12310"
     CDS             2294..>3083
                     /locus_tag="VFN42_12310"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET8997441.1"
                     /translation="MLFRQLFDTTSSTYTYLLASRRGGEALIIDPVLERVDRYLQLIR
                     ELDLKLVKAVDTHLHADHITGLGALRDRTRCITVMGEQSGVDVVSMRLTDGDTLRIEG
                     LALDVIYTPGHTDDSYSFALADRVFTGDTLLIRGSGRTDFQNGDPRAQYESIFHRLLK
                     LPEETLVFPAHDYKGDTVSTIGEEKRHNPRLQVKSADEYVALMNGLNLSNPKMMDVAI
                     PANMHQGLHQEEVARRGWAVSAEQASALLTHRDVALIDLRERSER"
BASE COUNT          541 a         1047 c          990 g          505 t
ORIGIN      
        1 accggttgct gcacccgcac cgcggcgcga tccgcgatcg cggcaatcag ggcatacatc
       61 agcgcatcga cggtgagcgc gtgccgcggt ccgatcggtg gcatgtcgac gagctgcccg
      121 tccaggagtt ccacccgatc gccggcggca aggatacccg cctcggcaag ccgatggtag
      181 tccgctaccg tgatccggtg ccgtggcaca gggtggcgaa gcaggatatc ggcgccgtcc
      241 attctggcct ctcttgctct gcgctgaact agcacaggtt gggacagtcc gcagagccag
      301 tcagatatgg gtgacgacgg cgatttcgga gatccccacc gcaccctgac catgtccgcc
      361 gcgtgggagg gacagggaag gccgggctca gtacatcgtg gacagcacgt tcgacaggcg
      421 cttgcgctcc tccagcgccc ggccggtgcc cagggcgacg cattgcagcg ggttctccgc
      481 cacgaccacc gccagtccgg tcgcgtcgcg cagcacctga tccagccggt acagcaacgc
      541 cccgccgccg gtcagcacga tgcccttgtc cacgatatcg gccgccagct caggcggggt
      601 gttctccagc gccaccttca ccgcttccac gatggcggaa accggctcgc tcaggctctc
      661 ggccacctgg cgctggccca ccagtacctc gcgcggcacg ccgttcatca gatcgcggcc
      721 acgcacttcg cggtacggac cgtcgtcgcc ttcctccggc gcgttcgccg cgccgatgtc
      781 catcttgatc cgttccgccg tgctctcgcc gatcagcagg ttatggttgc ggcggatata
      841 ggaaatgatc gcttcatcca tcttgtcgcc gccgacccgc acgctgcgcg aatagacgat
      901 gccgcccagc gagatcaccg ccacttcggt ggtgccgccc ccgatatcca cgatcatcga
      961 gccggacggc tcggtcaccg gcaggccggc gccgatcgcc gcggccatcg gctcctcgat
     1021 caactggacc cgccgtgcac cggcgctttc agcgctttcc tggatggcgc ggcgttccac
     1081 cgcggtggac ccggatggca cacaaacaat gatcaacggc gacgcgaaac cacgccggtt
     1141 atgcaccttg cggatgaaat gcttgatcat ttcctccgca acttcgaagt cggcgatcac
     1201 accatcgcgc aacggtcggc tggcggtaat atagccggga gtgcgcccaa gcatctgctt
     1261 ggcctcttcc cccactgcga ccacctgctt cttgccgcgg acatcagcga tggcgaccac
     1321 gctcggctcc gccagcacga tgccgcggcc cttgacgtaa accagagtat tcgccgtgcc
     1381 gagatcgatg gccatgtccg ccgacatgaa accgagcacg cgagaaaaca tgcagagggc
     1441 ctttgcagaa gctggaagca gcgaggaagg cggtcggctt agcccccgcg gggggcgtta
     1501 acaagggggt gatcggcggc ctcggctttg ccccattccg gccaccggaa atcctcgcac
     1561 caggggtgga aaaccgtcgc gaagcgctac caatcggagg agagccttat gaaacgtaag
     1621 atcgcctgcg gactggcttt ggcgatgatg cttggcttgg ccggctgcgg ctacagcccg
     1681 ggcacccgcg cggtgaccgg cggattgctg ggcgccggcg ccggcgcggg cattgcggcg
     1741 gccaccggtg gacgcccggc aaccggagcg gcgatcggcg ccggcgcggg cgccgtgggc
     1801 ggcgcagtga cctcaccctg aacaggaagg atgcaagaaa aactgacatg agcacgcgca
     1861 acaggaagtc gacgggccgc agcattgcca cgggcgtgct gctgaccgga ctggttctcg
     1921 ggctttccgg ctgcggatac agccggggtg accgggcggt gtcgggcggc ttgattggcg
     1981 ccggcggcgg ggccgccatc ggtgcgctga ccggcaatcc cctggctgga gccgcgatcg
     2041 gcggcgccgg cggtgcgctg gttggggccg caaccaaccc gcacgacgtg aacctcggcc
     2101 gtccggtctg gcgctgagca gcgggaccag gggagcccgg cgctcccctg gtccatattt
     2161 ttcccggcct cggcgctgcg cagcgttcga gagcatgggt tcgtcgatgc tggccaatct
     2221 ggaacagatt gccctatcct ccctgcctga atgagaaaga tcgatctcat cgggctgatc
     2281 ctgggagggg ccgatgctgt tccgccagtt attcgacacc acatcctcca cctacaccta
     2341 cctgctcgcc tcccggcgcg gcggcgaagc tctgatcatc gacccggtgc tggaaagggt
     2401 tgaccgatac ctgcagctca tccgtgagct cgacctgaag ctggtcaagg cggtggacac
     2461 ccatctgcat gccgaccaca tcaccggcct tggcgcactg cgcgaccgta cgcgctgcat
     2521 caccgtgatg ggcgagcaat ccggtgtcga tgtcgtctcg atgcgcctga cggacggtga
     2581 cacgctccgt atcgaggggc tggcgctgga tgtgatctac acgcccggcc acacagacga
     2641 ttcctacagt ttcgccctgg cagaccgcgt gttcaccggc gacacgctgc tgatccgcgg
     2701 ctccggccgc accgatttcc agaatggcga cccgcgcgcg cagtacgagt cgatcttcca
     2761 tcgcctgctg aagctgccgg aggagacgct ggtcttcccc gcgcacgact acaaaggcga
     2821 taccgtcagc accattggcg aggaaaagcg ccacaacccg cggcttcagg tgaaatcggc
     2881 ggacgagtac gtcgccctga tgaacgggct caatctgtcc aacccgaaga tgatggacgt
     2941 cgcgatcccc gccaacatgc accagggact gcatcaggaa gaagtcgccc ggcgcggttg
     3001 ggcggtcagc gccgaacagg catccgcact gctgactcat cgcgacgtag cgctgatcga
     3061 tctgcgggaa cgcagcgagc ggg
//
LOCUS       DASSZH010000568         3632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_490620, whole genome shotgun sequence.
ACCESSION   DASSZH010000568 DASSZH010000000
VERSION     DASSZH010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490620"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="VFN42_12315"
     CDS             complement(<1..1095)
                     /locus_tag="VFN42_12315"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET8997442.1"
                     /translation="MLSGLPPIAFAVPILVVTYILVMIDRFDRSILALLGGGAMVLTG
                     VLTQQQAITGIDFNTIGLLTGMMMLVAISRRSGIFEYLAIRAVQIARASPAGLLVSLA
                     LVTALLSALLDNVTTVLLIAPVTIAICRRLEVPPFPFLISEVLASNFGGTATLIGDPP
                     NILIGTAANLSFNDFIVHMAPCVLLVLAAQAALMHLVQGRHLRTNAKARQEVMAIRAA
                     SSIVDPRLLRHSIVVFALTLTGFCTQRVIHIEPGTIALIAAAALMLLENLVHPREEQF
                     AKVSATYAEVDWITIFFFIGLFIVVRGVEVTGALGWLAQEIVAATSGNFGMTLSIVLW
                     VSAILSALIDNVPFVAAMIPVLHAMAPQFGG"
     gene            1236..2516
                     /locus_tag="VFN42_12320"
     CDS             1236..2516
                     /locus_tag="VFN42_12320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET8997443.1"
                     /translation="MTVESASQLHVCVIGAGIVGAASAVELLRDGHRVTIVEPGEPGG
                     EQAASYGNGTLLNPSSIIPMTAPRLWRKVPGYLADPLGPLTIRWSYLPRLLPWLRRFL
                     SAGATEAKVAAIARALRPLLLDAPERHRRLAEEAGVGDLISRQGVLFPFPSRADFEAE
                     ALSWRLRRENGVRWLELSEDELRQREPALDRRYRFALLVEDNGQCRDPGAYVAALVRH
                     VVAQGATLHRGRATGFRIAHSHLQAVRTDAGEIACDRAVIAAGARSKALAAAAGDRVP
                     LETERGYHAVIAAPGVAPRYPVLTSDTKAAGVMTPQGLRIAGQVELAGLDAAPNWQRA
                     EIMRDLARRTFSGVPADLPADRVKLWMGHRPSMPDGLPCLGPASGCADVVHAFGHGHV
                     GLTAGAATGRIVADLISGRDPGLDLAPYSARRFN"
     gene            2635..>3632
                     /locus_tag="VFN42_12325"
     CDS             2635..>3632
                     /locus_tag="VFN42_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET8997444.1"
                     /translation="MHGSTASTSRFSGLTPLLAPRSVAVLGASSDPTRISGRPIAYMK
                     AQGFQGGLYPINPNRSEVQGLKAYASIGDLPEVPDVVIVAVAAEVAEKSIEELASRGV
                     KAVVMFTAGFAEMDEAGEAAQHRMVATARQHGMRLLGPNCLGVFDARTAYYATFSSSF
                     DSGWPVMGRIGIASQSGAYGTHLYTLARNRGIGASLCIMTGNEADVTVGECIGWLAEN
                     PEVDVIAAYIEGIRESASLIAAFEAARAAKKPIIMQKVGRSELGSKAAKSHTASIAGD
                     DAVTEAVMREFGVARARNSEEMLDIAHTATRKIYPARNSLGVITVSGGAGVLISDTA"
BASE COUNT          583 a         1222 c         1310 g          517 t
ORIGIN      
        1 gccgccgaat tgcggtgcca tcgcgtgcag caccggaatc atggccgcga cgaagggcac
       61 gttgtcgatg agcgccgaca ggatcgccga gacccacagc acgatgctga gcgtcatgcc
      121 gaaattgccg gaggtcgccg cgacgatctc ctgcgccagc cagcccagcg cgccggtgac
      181 ctccacaccg cgcaccacga tgaacaggcc gatgaagaag aagatggtga tccagtccac
      241 ctcggcatag gtggcggaga ccttggcgaa ctgctcctcg cgcgggtgca ccaggttctc
      301 cagcagcatc agcgccgccg cggcgatcag cgcaatcgtg cccggctcga tatggatcac
      361 acgctgcgtg cagaagccgg tcagggtcag cgcgaacacc acgatcgagt gccgcagcag
      421 gcggggatcg acgatggagc tggcggcgcg gatcgccatc acctcctgcc gcgccttggc
      481 gttggtacgc aggtgccggc cctgcaccag gtgcatcagc gcagcctgcg cggccagcac
      541 cagaagcacg cagggcgcca tgtgcacgat gaaatcgttg aagctgagat tggcggcggt
      601 gccgatcagg atgttcggcg gatcgccgat cagcgtcgcg gtgccgccga aattcgacgc
      661 cagcacttcc gagatcagga acggaaacgg cggcacctcc agccggcggc agatcgcgat
      721 cgtcaccggg gcgatcagca gcaccgtggt gacattgtcg agcagcgcgg acagcagcgc
      781 ggtgaccagc gccagcgaca ccagcagccc ggccggtgag gcgcgcgcga tctgcaccgc
      841 gcggatcgcc aggtattcga agatgcccga ccggcgggag atcgccacca gcatcatcat
      901 gccggtcagc aggccgatgg tgttgaagtc gatgccggtg atcgcctgct gctgcgtcag
      961 cacgccggtc agcaccatcg cgccgccccc gagcagtgcg aggatggaac ggtcgaaccg
     1021 gtcgatcatc accaggatgt aggtgaccac caggatcggc acggcaaacg cgatcggcgg
     1081 caggcctgac agcattggcg ggtccgtgac agcgatgcga agggttttcg gggatttgcc
     1141 gcggcggtgc cagggggagg gcagggcagc cgggtcatca taccgggatg acggctgctg
     1201 cttcgcggca taggctcggc ggcgccgaag ggggaatgac cgtggagtcc gcatcccagc
     1261 tacatgtctg tgtcatcggt gccggcatcg tcggcgctgc cagcgcggtg gaactgctgc
     1321 gcgacggcca ccgcgtcacc atcgtcgagc ccggcgagcc gggcggcgag caggcggcca
     1381 gctacggcaa cggcacgctg ctgaacccca gctcgatcat cccgatgacg gcgccgcgtc
     1441 tgtggcggaa ggtgcccggc tacctggccg atccgctggg gccgctgacc atccgctggt
     1501 cgtatctgcc gcggctgctg ccctggctgc ggcgcttcct cagcgccggg gcgaccgagg
     1561 cgaaggtggc ggcgatcgcc cgcgcgctgc gcccgctgct gctggatgcg ccggaacgcc
     1621 atcgccgcct ggcggaggag gccggcgttg gtgacctgat ctcccgccag ggcgtgctgt
     1681 tcccctttcc ctcgcgcgcg gatttcgaag cggaggcact gtcctggcgg ctgcgccggg
     1741 agaacggtgt gcgctggctg gagctgtcgg aggacgagct gcggcaacgc gaaccggcgc
     1801 tggaccggcg ctatcgcttc gcgctgctgg tggaagacaa cggccagtgc cgcgatcccg
     1861 gcgcctatgt ggcggcgctg gtgcggcatg tggtggcgca gggcgcaaca ctgcatcgcg
     1921 ggcgcgccac cggcttccgc atcgcgcatt cccacttgca ggcggtgcgc accgatgcgg
     1981 gggagatcgc ctgcgaccgc gcggtgatcg ccgcgggggc gcggtcgaag gcgctggccg
     2041 cggcggcggg cgaccgcgtg ccgctggaga ccgaacgcgg ctaccacgcg gtgatcgcgg
     2101 cgcccggcgt cgccccgcgc tatccggtgc tgaccagcga caccaaggcg gccggcgtga
     2161 tgacgccgca ggggctgcgg atcgccggcc aagtggagtt ggccgggctg gacgcggcgc
     2221 cgaactggca gcgggcggag atcatgcgcg atctggcgcg gcgcaccttt tcgggtgtgc
     2281 cggcggatct gccggcggac cgggtgaagc tgtggatggg gcaccggccg tcgatgccgg
     2341 acgggctgcc ctgcctgggg ccggcctccg gctgtgccga cgtggtgcac gcgttcggcc
     2401 acggccatgt cgggctgacc gccggcgcgg cgaccgggcg gatcgtggcc gacctgatca
     2461 gcggccgcga tccggggctg gacctggcgc catactccgc gcggcggttc aactgacggg
     2521 ccgggctgcg ccgcgcgccg gcctcgccgt cttgccgccg tccggaccca aggtcacact
     2581 ccccgccttc acgcaacggc aacagccgag cgcagacagg ggaaatgtcc gcccatgcac
     2641 ggttccaccg cctcgaccag ccgcttctcc ggcctgaccc cgctgctcgc gccgcgttcc
     2701 gtcgccgtgc tcggcgcctc cagcgacccg acgcgcatca gcggccggcc gatcgcctac
     2761 atgaaggcgc agggcttcca gggcgggctg tacccaatca atccgaatcg cagcgaggtg
     2821 caggggctga aggcctatgc ctcgatcggc gacctgccgg aagtgccgga cgtggtgatc
     2881 gtggcagtcg ccgcggaagt cgcggaaaag tcgatcgagg aactcgcctc ccgcggcgtg
     2941 aaggccgtgg tgatgttcac cgccggcttc gcggaaatgg atgaggccgg cgaggcagcc
     3001 cagcaccgca tggtcgccac cgcgcgccag cacggcatgc gtctgctcgg ccccaactgt
     3061 ttgggcgtgt tcgacgcacg caccgcgtat tacgcgacct tctcgtcgtc cttcgattcc
     3121 ggctggccgg tgatgggccg catcggcatc gccagccagt ccggcgccta cggcacgcat
     3181 ctctacacgc tggcgcgcaa ccgcggcatc ggcgcgtcgc tgtgcatcat gaccgggaat
     3241 gaggccgacg tgaccgttgg cgaatgcatc ggctggctgg cggagaatcc ggaagtcgat
     3301 gtgatcgccg cctatatcga gggcatccgc gaatccgcca gcctgatcgc cgccttcgaa
     3361 gcggcgcgtg cggcgaagaa gccgatcatc atgcagaagg tcggccgcag cgaactcggc
     3421 agcaaggcgg caaagtcgca taccgcatcg atcgccggcg acgatgcggt gaccgaggcg
     3481 gtgatgcgcg aattcggcgt ggcgcgcgcg cgcaactcgg aagagatgct ggacatcgcc
     3541 cacaccgcaa cgcggaagat ctatccggca cgcaactcgc tgggggtgat caccgtgtcc
     3601 ggcggcgccg gcgtgctgat cagcgacacc gc
//
LOCUS       DASSZH010000569         6484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_422313, whole genome shotgun sequence.
ACCESSION   DASSZH010000569 DASSZH010000000
VERSION     DASSZH010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6484
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422313"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="VFN42_12330"
     CDS             complement(<1..583)
                     /locus_tag="VFN42_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006557542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HET8997445.1"
                     /translation="MTSHTDIAAEGWVALLPAGLRPYLLLARVDRPIGVWLLFLPGLW
                     GILLAGPRPHTLRLILLFALGSLVMRAAGCVVNDLWDRDIDRKVARTAGRPLASGQLR
                     ARHALAFLGVLLAIGLVILLQLNPLSQVLGVSSLVLVALYPAAKRVTWWPQLVMGFTF
                     GFGAPLGYAAGADRLDWSWALLYGAAILWDLGFD"
     gene            complement(657..1025)
                     /locus_tag="VFN42_12335"
     CDS             complement(657..1025)
                     /locus_tag="VFN42_12335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8997446.1"
                     /translation="MQADGRAIALVDDDSAVRDSMRFLLEIEGYDILDFPSAAEFLDE
                     CALDRIRGLILDHHMPLMTGLELAAHLRKQGNAIPILLITGSPSPAIVARAAELGIDK
                     VLEKPPSETDLLQFVANLPA"
     gene            complement(1129..2634)
                     /locus_tag="VFN42_12340"
     CDS             complement(1129..2634)
                     /locus_tag="VFN42_12340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET8997447.1"
                     /translation="MTSATESLDPNLLPLLDLAQAMVRDLDGRITHWTSGAEHLYGWS
                     RQEAVGQIAHDLLATEFPVPLDEIQASLLRESQWHGELRHRTREGRTIQVASHWTLRR
                     GTGGQPDSVAEVNNDITAQSRAEDALLRLAAIFASSNDAIIGMTLDGAITDWNEAAAS
                     LFGYSAAEIMGQPVTVLYPPERMGEELTLLGRIRRGEPVSHFETIRRRRDGSTLPVSV
                     NLSAILDASGTVIGASKIVRDLSESQARDKRLAEVQAELTHVSRLIELGQLVSSLVHE
                     VNQPLTAIGNYTQAIQRLLAAGNAERVPLALEKIVEQTERAYQIIQRLRDFVRKGQSQ
                     RRAERLGETIQEAIALAAIGPAARGATIRTEVAPGVDRVEIDRIQIEQVLFNLLRNAL
                     EAMQGQPRREVLVRAQPAADGMVEVAVADSGPGLPDTVRQKLFQPFVTTKAAGMGVGL
                     SVCKGIVEAHGGQMTADDRPGGGTVFRFTVPAVSAAQAAAMGNSRGVEQPC"
     gene            complement(2672..3613)
                     /locus_tag="VFN42_12345"
     CDS             complement(2672..3613)
                     /locus_tag="VFN42_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8997448.1"
                     /translation="MTTETASAGHGRPAAVRHYVVRTPTLEIAYEASGPEDGTPLILL
                     HGFPDDPRAYDGVAPPLAAGGCRVLVPYLRGYGPTRFCDPATPRSGQQAALGADLRDF
                     MDALGIQRALLAGYDWGGRAACVVAALWPERVRGLVSVTGYNIQDIAAAARPAAAEQE
                     LRYWYQWYFHTERGRAGLVQNRREICRLLWRLWSPEWQFDDATFAATAASFDNPDFVD
                     VVIQSYRHRYGNAPGDPALEDIERRLAALPPITVPAVALHGEADGVGPVEQSASHGRH
                     FTGPYERRIIPHAGHFLPHETPEAMVVALNDLLKRRD"
     gene            complement(3613..4359)
                     /locus_tag="VFN42_12350"
     CDS             complement(3613..4359)
                     /locus_tag="VFN42_12350"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8997449.1"
                     /translation="MSGLLDGKLALITGAGSGIGAGIARGMAAAGARVLVADINGDAA
                     TGVAGTIGGGAESYTLNVTDRDACDAIAGAIRREHGPIDILVNNAGILRRGKVDAGSS
                     RHDWDATLAVNLDGPYNVTTAFLDQLKDTKGAVINIGSIQSFVALPNSAAYTASKGGV
                     RALTKALAIELSPLGIRVNAIGPGMIATPLNARARENPDYMRNFEGRIPLGRIGTPED
                     IAGPAVFLASDLARYITGVTLPVDGGFLAY"
     gene            4463..5674
                     /locus_tag="VFN42_12355"
     CDS             4463..5674
                     /locus_tag="VFN42_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8997450.1"
                     /translation="MRQSASDAPQSASHIRGIPSTVWALGFVSLFMDVSSELIHALLP
                     LFLVDRLGTTLLTVGLIEGVAEATAAITKVFSGAVSDWLGRRKLLALFGYGLSALSRP
                     LFPLADSAGMVLAARFIDRLGKGIRGAPRDALVADVTPPEQRGAAYGLRQALDSVGAF
                     LGPLLAVLLMTAYADHIRAVLWWAMIPAWISVLLIVFAVREPGTCGTQRKGVPIRRPD
                     VAALPRGYWIAVLIGVLFTLARFSEGFLILKGQHAGLTITLVPLVLVGMNFVYAAASL
                     PVGKLSDRLGRRGLLALGMLVLAGADFVLAAVPGLIGLFSGVALWGLHMALSQGLLAA
                     LVADTAPPALRGTAFGVFNLATGVVLLLASLLAGALWQAIGPDATFAAGAGFALAAAI
                     GLLLMLSRRES"
     gene            complement(5654..6337)
                     /locus_tag="VFN42_12360"
     CDS             complement(5654..6337)
                     /locus_tag="VFN42_12360"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET8997451.1"
                     /translation="MAAPILFAGDPHRNFAPILRACAARQPGALILLGDCECDAPLPQ
                     LLAPAMQAGWRVYWVLGNRDTDTPDTYDHLVAGAPEGNLGGQVRRIERVRIAGLPGVF
                     KPRVWHPVEAEPPGFRTRAEFLAVLPQREQWRGGLPLWHRDTIFPEDFAALQTQRFDV
                     LVTHEAPSSHRHGFAVIDRLAQAGGARLIVHGHHHQAYDAVLPNGIRVRGLGLAEPWE
                     LPRSGFPAG"
BASE COUNT          987 a         2234 c         2217 g         1046 t
ORIGIN      
        1 tgtcgaaccc caggtcccac agaattgccg ctccgtacag cagcgcccag gaccagtcca
       61 gccgatccgc ccccgctgcg tatcccagcg gcgccccgaa cccgaacgtg aaccccatga
      121 caagttgcgg ccaccacgtc acgcgcttgg cggcggggta caacgccacc agcaccagcg
      181 aggacacccc cagcacctgc gacagcgggt tgagctggag caggatcacc aggccgatag
      241 ccagcaatac gcccaggaac gcgagcgcgt gccgggcccg caactggccc gacgccagcg
      301 ggcggccggc ggtgcgcgcc accttgcggt cgatgtcgcg atcccacagg tcgttcacca
      361 cgcagccggc ggcacgcatc accaggctgc ccagtgcgaa cagcagaatc agccgcagcg
      421 tgtgcggacg cggaccggcc agcaggatgc cccacagccc cggcaggaac agcaaccaga
      481 cgccgatcgg ccggtcgacc cgcgcaagca gcagataggg ccgcaagccg gcgggcagca
      541 gagccaccca gccctccgcg gcaatatcgg tatgggatgt catggcggca ttttgcgccg
      601 cgcgggcgcg atgcggaagg cggcatgcat gcgccgccgc ccgggcaaag ccgccgctat
      661 gcgggcaggt tggcgacgaa ctgcagcagg tcggtctcgg acgggggctt ctccagcacc
      721 ttgtcgatgc ccagttccgc cgcgcgcgcc acgatcgccg gggacggcga gccggtgatc
      781 agcaggatgg ggatggcgtt cccctgcttg cgcagatgcg cagccagttc cagcccggtc
      841 atcagcggca tgtggtggtc gagaatcagg cccctgatcc ggtccagcgc gcactcatcg
      901 aggaactcgg ccgccgacgg gaaatccagg atgtcgtacc cctcgatctc cagcaggaag
      961 cgcatcgaat cacgcacggc agagtcgtcg tcgaccagtg cgattgcacg accatccgcc
     1021 tgcatcagct agtcccttcc ggattggctc aactgacgcc cgagcgtccg cgatctcgcc
     1081 acctgatcgc catgtacaca ctcgggcgcg gcatcggatt gattcagatc aacaaggctg
     1141 ttcgacacca cgggaattgc ccatagcggc ggcttgcgct gcggagacgg ccgggacggt
     1201 gaagcggaac accgtgccgc cgcccgggcg gtcgtctgcc gtcatctgcc cgccatgcgc
     1261 ttccacgatg cctttgcaga cggacaatcc gacgcccatg ccggccgcct tggtggtgac
     1321 gaaaggttgg aacagcttct gccgcacggt gtccggcaga cccggcccgc tatcggccac
     1381 cgcgacctcc accatgccat ccgccgccgg ctgcgcacgc accaacactt cgcgccgggg
     1441 ctgaccctgc atggcctcca gcgcattgcg cagcaggttg aacagcacct gctcgatctg
     1501 gatgcggtcg atctccaccc ggtccacccc gggcgcgacc tccgtccgga ttgtcgcacc
     1561 tcgcgccgcc ggaccgatcg ccgccagcgc gatcgcctcc tggattgtct cacccagccg
     1621 ttccgcccgg cgctgcgact gtcccttgcg cacgaaatcg cgcagccgct ggatgatctg
     1681 gtaggcacgt tcggtctgct cgacgatctt ctccagcgcc agcggcacgc gttcggcatt
     1741 gcccgccgcc agcaggcgct ggatcgcctg ggtgtagttg ccgatcgcgg tcagtggctg
     1801 gttcacctcg tgcaccagcg aggacaccag ttgcccaagt tcgatcagcc gcgacacgtg
     1861 ggtgagttcc gcctggacct cggccagccg cttgtcgcgc gcctggcttt cgctcagatc
     1921 gcgcacgatc ttcgaggcgc cgatcaccgt gccactggca tccaggatcg ctgacagatt
     1981 caccgagacg ggaagcgtgc tgccatcgcg gcgccggcgg atggtttcga agtggctgac
     2041 cggctcgccg cggcggatgc ggccgagcag cgtgagttcc tcccccatcc gctcgggcgg
     2101 atagagcacg gtcaccggct gccccatgat ctccgccgcg ctgtagccga acagcgaagc
     2161 cgcggcttca ttccagtctg tgatcgcgcc gtccagcgtc atgccgatga tggcgtcgtt
     2221 ggaggaggcg aaaatggcgg cgagccgcag cagcgcgtcc tcggcgcggc tttgcgcggt
     2281 gatgtcgttg ttgacctccg ccactgaatc gggctgtccg ccggtgccgc gccgcagcgt
     2341 ccagtggctg gcgacctgga tggtgcgtcc ctcccgcgtg cggtggcgca attcgccatg
     2401 ccactgcgat tcacgcagca ggctagcctg gatttcgtcc agcggcaccg gaaactcggt
     2461 ggcgagcagg tcgtgcgcaa tctggccgac cgcctcctgt cgcgaccagc cgtagagatg
     2521 ctccgcgcca ctggtccagt gggtgatgcg cccgtcaagg tcgcgcacca tcgcctgcgc
     2581 caggtccagt aacggcagca ggtttgggtc gagcgattca gttgcggaag tcacgcggga
     2641 aaggctccag ggtcacgcag cctgcattgc gtcagtcgcg gcgttttagc aagtcgttta
     2701 gggccacgac catcgcctct ggtgtttcat gcggcaggaa gtggccagca tgtggaatga
     2761 tacgccgttc atagggcccg gtgaaatggc ggccatggct ggcggactgc tccaccgggc
     2821 cgacgccgtc agcctcgcca tgcagcgcaa ctgccgggac ggtgatcggc ggcagtgccg
     2881 caagccgccg ctcgatgtcc tccagggcgg gatcgcccgg cgcattgccg tagcggtggc
     2941 gatacgactg gatcaccaca tcgacgaaat ccggattgtc gaagctggcc gcggtggcgg
     3001 cgaaggtggc gtcgtcgaac tgccactcgg gtgaccacag ccgccacagc agccggcaga
     3061 tctcccgccg gttctgcacc aggccggcgc ggccgcgttc cgtatggaaa taccactggt
     3121 accagtagcg gagttcctgt tccgccgccg ccggccgcgc cgcggcggcg atgtcctgga
     3181 tattgtagcc ggtgaccgag accagtcccc tcacccgctc cggccacagt gccgcgacga
     3241 cgcaggctgc tctaccgccc cagtcatagc cggccagcag cgcccgttgg atgcccagtg
     3301 catccatgaa gtcccgcagg tcggcgccga gcgcggcttg ctggccggag cgcggcgttg
     3361 ccggatcgca gaaacgggtc ggtccgtagc cgcgcagata gggcaccagg acacggcagc
     3421 cgcccgccgc caatggtggc gcaacgccgt cataggcacg cgggtcgtcg gggaaaccgt
     3481 gcagcaggat cagtggcgtg ccgtcctccg ggccgctggc ctcgtaggcg atctccagcg
     3541 tcggggtgcg taccacgtag tggcggacgg cagccggccg gccatgcccc gcggaagcag
     3601 tctcggtggt catcaatacg ccaggaatcc gccatccacc ggcagcgtca cgccggtgat
     3661 gtagcgcgcg aggtcggagg ccaggaacac cgccgggccg gcaatgtcct ccggcgtgcc
     3721 gatacggccg agcgggatgc ggccctcgaa gttgcgcatg tagtccgggt tctcccgcgc
     3781 tctggcgttc agcggggtgg cgatcatgcc gggaccgatg gcgttgacgc gaatgcccag
     3841 cggcgacagc tcgatcgcca gcgccttggt cagcgcgcgg acgccgcctt tcgaggcggt
     3901 gtatgcggcc gaattcggca aggcgacgaa ggactggatg gagccgatat tgatgaccgc
     3961 acccttggtg tctttcagct ggtccaggaa ggcggtggtg acattgtacg gtccgtcgag
     4021 attcaccgcc aaggtcgcat cccaatcgtg ccgggagctg ccggcatcca ccttgccgcg
     4081 gcggaggatg ccggcgttgt tcaccaggat gtcgatcggc ccgtgttcgc gccggatggc
     4141 gccggcgatg gcgtcacagg catcgcggtc ggtaacgttc agcgtgtagg actcggcgcc
     4201 gccgccgatc gttcccgcga cgccggtggc cgcgtcgcca ttgatgtccg cgaccagcac
     4261 gcgggctccg gccgccgcca tgccccgcgc gatcccggcg ccgatgccgg aacccgcccc
     4321 ggtgatcagc gcaagcttgc cgtccagcaa tccgctcatg gcgcttccct tcttccgcgc
     4381 cgggagtctg ccgcgatacg cccggcaaca gaagcccccc tgccggtgag gacatcaccg
     4441 cgcggcgttg tagggtggcc ggatgcgaca atccgcttcc gatgcaccgc aatccgccag
     4501 ccacatccgc ggcatcccat ccaccgtctg ggcgctcggg ttcgtttcgc tgttcatgga
     4561 cgtctcctcg gagctgatcc acgccttgct gccgctgttc ctggtggacc ggctgggcac
     4621 gacgctgctg acggtggggc tgatcgaggg cgtggccgag gcgaccgcgg cgatcaccaa
     4681 ggtgttctcc ggcgcggtgt ccgactggct gggccggcgc aagctgctgg ccctgttcgg
     4741 ctacgggctg agcgccttgt ccaggccgtt gttcccgctc gccgactcgg ccggcatggt
     4801 gctggcggca cgcttcattg accggctggg caaaggcatc cgcggtgcac cgcgcgacgc
     4861 gctggtggcc gatgtcacgc cacccgagca gcgcggcgcc gcctatggat tgcgccaggc
     4921 gctcgacagc gtcggcgcct tcctgggccc gttgctggcg gtgctgctga tgaccgccta
     4981 cgcggatcat atccgcgcgg tgctgtggtg ggcgatgatc cccgcctgga tctcggtgct
     5041 gctgatcgtg ttcgcggtgc gcgagccggg gacctgcggc acacagcgca aaggcgtgcc
     5101 gatccggcgg ccggatgtgg ccgccttgcc gcgcggctac tggatcgccg tgctgatcgg
     5161 cgtgctgttc accttggccc gcttcagtga gggctttctg atcctgaagg ggcagcacgc
     5221 cgggctgacg atcaccctgg tccctctggt gctggtgggc atgaacttcg tgtatgcggc
     5281 agcgtcattg ccggtgggca agctctcgga ccggctgggg cgcagaggtt tgctggccct
     5341 gggcatgctg gtactggccg gcgcggattt tgtgctggcg gcagtgccgg ggctgatcgg
     5401 gctgttcagc ggcgtcgcac tgtggggcct gcacatggcc ctgtcgcagg ggttgctcgc
     5461 ggccctggta gccgataccg caccgccggc gctgcgcggc accgctttcg gcgtattcaa
     5521 cctggcgacc ggcgtggtgc tgctgctcgc cagcctgctg gccggtgctc tgtggcaggc
     5581 gattgggccg gatgcgacct ttgccgccgg cgcgggattc gccctggcgg cggcgatcgg
     5641 cctgctgctg atgctatccc gccgggaatc ctgaccgcgg cagttcccac ggctccgcca
     5701 gtccaagccc gcgcacgcga atgccgttgg gcagcacggc gtcatatgcc tggtgatgat
     5761 gcccgtgcac gatcagccgg gcaccgccgg cctgcgccag ccggtcaatg acggcaaagc
     5821 cgtggcgatg cgacgatggg gcttcgtgcg tcaccagcac gtcgaagcgc tgggtctgca
     5881 aggcggcgaa gtcttccgga aagatcgtgt cgcggtgcca cagcggcagc ccgccccgcc
     5941 actgctcgcg ttgcggcagc accgcgagga attcggcccg cgtgcgaaag cctggcggct
     6001 cggcttccac cgggtgccag acacgaggct tgaacacgcc gggcagtccc gcaatccgca
     6061 cccgctcgat ccgcctgacc tgtccaccga gatttccctc cggcgcgccg gcgaccagat
     6121 gatcgtaggt gtccggcgtg tcggtgtcgc gattgccgag gacccagtag acgcgccagc
     6181 ccgcctgcat cgccggtgca aggagctgcg gcagcggcgc atcgcattcg caatcgccca
     6241 gcaggatcag cgcaccgggc tggcgcgccg cgcaggcgcg caggatcggt gcgaagttgc
     6301 ggtgcggatc accggcgaac aggatcggcg cggccatgcg ggatcagtgt cgccccggcc
     6361 aggcgccatc ctcatccaca aggccgggcg ggacggtgac cgggacggag ctgccgggca
     6421 tgggcttcct tggcgagtgg cgggggcggc cgctcgtgga gtcagccgtc cagcacggca
     6481 cgta
//
LOCUS       DASSZH010000570        10051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_968263, whole genome shotgun sequence.
ACCESSION   DASSZH010000570 DASSZH010000000
VERSION     DASSZH010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 10051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10051
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968263"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /gene="uppS"
                     /locus_tag="VFN42_12365"
     CDS             complement(<1..550)
                     /gene="uppS"
                     /locus_tag="VFN42_12365"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HET8997452.1"
                     /translation="MDGNGRWAASRRLPRIAGHREGARAVRRTIEAAINSGVGWLTLY
                     AFSSENWRRPVTEVLDLTGLLRNYLRTEIAELKANGVRLRIIGEPGRFDPDIQRDLTD
                     AERATEANTRLNLTVALSYGARAEIAAAARAIAQAAVAGRLDPNTLDEARLSGFLTTA
                     GMPDPDLVIRTSGEQRLSNFLLW"
     gene            complement(648..1220)
                     /gene="frr"
                     /locus_tag="VFN42_12370"
     CDS             complement(648..1220)
                     /gene="frr"
                     /locus_tag="VFN42_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET8997453.1"
                     /translation="MAAPPDIDTLRQDITRRMDGALETLKREFNGLRTGRAHPGLLEP
                     VKVHAYGTDMPLTQVGTVGAPEPRMLTVQVWDRALVSAVEKAIRESGLGLNPATDGQT
                     VRVPLPQLTTERRNELVKTAHKYAEGARVAVRGVRRDGMEQIKGYEKKHDIGEDVAKD
                     WHDVVQKLTDQYIKKVDETLADKEKDIRQV"
     gene            complement(1259..1981)
                     /gene="pyrH"
                     /locus_tag="VFN42_12375"
     CDS             complement(1259..1981)
                     /gene="pyrH"
                     /locus_tag="VFN42_12375"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET8997454.1"
                     /translation="MSSPLYKRVLLKLSGEALMGRREYGLDNDMVRAIATDIGGVVAM
                     GVQVCVVIGGGNIFRGVSGAAAGMERAQADYMGMLATVMNALGLQSALEKLGIPTRVQ
                     SAIPMSSVCEPYIRRRAERHMEKGRVVIFAAGTGNPFFTTDTAAALRAAEMNCDALLK
                     GTQVDGVYSADPRKVADARRYEQLTYLDVLANDLGVMDAAAISLARENGLPIVVFNIH
                     APGAFAAVMQGEGRFTRIIEPK"
     gene            complement(2319..5945)
                     /gene="nifJ"
                     /locus_tag="VFN42_12380"
     CDS             complement(2319..5945)
                     /gene="nifJ"
                     /locus_tag="VFN42_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HET8997455.1"
                     /translation="MLRNPATMDGNTAVAHVAYRVNEVCAIFPITPSSTMAELADEWA
                     AQGVKNIWGNLPIVQEMQSEGGAAGAVHGALQSGALTTTFTASQGLLLMIPNMFKIAG
                     ELTSTVFHVAARSIATQALSIFGDHSDVMAVRSTGFALLSSGSVQEAHDAALIAQAAT
                     LASRVPFLHFFDGFRTSHEVGTIDLLSDAQIRAMIDDSLVRAHRARAMSPEHPFVRGT
                     AHNPDTFFQARETVNPYYAAVPEIVQRAMNRFAGLTGRQYRLMEYTGAPDAERVVVVM
                     GTGAETARIAAGALNARGEKVGVLQVRLYRPFPSAALLAELPATVRAIAVLEQTKEPG
                     SLGEPLYLDVVTALAQAGRLPRSIIGGRYGLSSKDFGPPLAKAVFDELNRDKPRNGFT
                     LGIHDDLSQTSLEPDAAFQVEPPGVVSALFYGLGSDGTVGANKNSVKIIAEDAGLHAQ
                     GYFVYDSHKSGAQTVSHLRFGPQPIHAPYLIERASFIGIHQFQFVTRQDMLKLAAPGA
                     TLLLNAPYGADTLWDHLPRDMQRRIIDLKLKLYVIDASGVAERAGLRGRANTVLQTCF
                     FALSGVLPRERALDHIKTAIRKTYARRGEAVVASNFAAVDAALDHLEEVPVPATVSSN
                     WERQPAVPANAPDFVREVTALMLEGRGDEIPVSKLPVDGTWPSGTAAWEKRNVSDLVA
                     TWDQDLCIQCGQCSFVCPHGVIQGRYFDAAKLEGAPDGFKSAPINVRGFPDVRFTLQF
                     SIEDCTGCLLCHEACPAVRPGTDAKAIVMRDKAPLIEAERANRNFFDRLPVNDRARVD
                     FANVRGVQFLEPLFAYSGACAGCGETPYLKLLSQLFGDRAQVANATGCSSIYGGNLPV
                     TPWSANREGRGPAWNNSLFEDNAEFGLGFRLAADQHVELARRLVQQLSPQLGDGLVQA
                     ILQAPQVQESEIRAQRERVAELTRRLRTFNGTPAAQDLLSVVDHLVRRSVWIVGGDGW
                     AYDIGYGGLDHVLASARDVNVLVLDTEVYSNTGGQASKATPLGAVAKFAAAGKRTARK
                     DLALQAIAYGNVYVAQVAMGANPQQTLQAFREAEAYPGPSLILAYSHCIAHGIDMQFG
                     MQQQEKAVASGYWPLFRYNPAMRAHGANPFVLDSPRPTMPFRDYALQEIRYKSLLQTR
                     PEEAETLLAAAQAAVEEKYRTYEEMAGWSAARFHPVGLERGSTPDKRLPATPQDWEHI
                     GVRG"
     gene            complement(6127..7773)
                     /locus_tag="VFN42_12385"
     CDS             complement(6127..7773)
                     /locus_tag="VFN42_12385"
                     /inference="COORDINATES: protein motif:HMM:NF009410.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HET8997456.1"
                     /translation="MSTTARDLTPVVDLTSEKGTGPFRTRRPLYVDLLPPCNNACPAG
                     ENIQAWLALAQAGEYRAAWETLVADNPLPATHGRVCYHPCETGCNRGHLDSAVSIHAV
                     ERFLGDMAKAQGWDYPPGAPQSGKRVLVVGAGPSGLSAAYHLTRMGHQVEIHEAGPVA
                     GGMLQFGIPAYRLPRAELAAEVARIERMGVKIVLNHPVTDVLAERDAGKFDAVFVAIG
                     AHVAQHVDIPARDAVRVLDAVSMLRETGTGGKPMLGRRVVVYGGGNTAMDAARTAKRL
                     GASEALIVYRRDRQHMPAHEFEADEALDEGVKIKWLTTIREIVGPTLTVERMALDDKG
                     RPQPTGEFETLEADAVVLALGQVSDSAFLRSVSGVDVKPDGVVAVDRNMMTGHPGIFA
                     GGDLVPCERTVTVATGHGKLAARNIDAWLRGGRYNALQRPEVVNFAMLHLPVFSDVEP
                     APQRRLEEEERLSGFEEVLSGYSEPEARREARRCLSCGVCYECDNCFAACPEGAIIKL
                     GPGNGYQVDTRLCTGCAVCFEQCPCHAIEMVAEAGFVELP"
     gene            complement(7798..8274)
                     /locus_tag="VFN42_12390"
     CDS             complement(7798..8274)
                     /locus_tag="VFN42_12390"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET8997457.1"
                     /translation="MALVASEIMTQNVVSALPNATVAQLARLMADNGISAIPICEKDG
                     TLLGMVSEGDLIPAIGQKPADRQAWWLQVLAEGTDLAPEFLEYMRLDHRPAEKFMQRA
                     VITATERSSATEIADLLSHHHIKRVPILREGKLVGIVSRADLIRAIVRQPDALSRD"
     gene            complement(8302..8880)
                     /locus_tag="VFN42_12395"
     CDS             complement(8302..8880)
                     /locus_tag="VFN42_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997458.1"
                     /translation="MVGITLSAEQIRSAPPEVRQWLEQQIAALLSPLPVQPPHPGPKL
                     VRCSRAELQAILGQLQNLLPVVSVFFELGREAASVPVQGMRVLRLADILRHTRLQTVD
                     QVVECLEVITDVLRRLRHDSDAMLCAVDAEGRCYIAEETAANILALWRDIVAARALQP
                     AEPSAVPAAPGDLLPPFRVAATPDTAPVAEPG"
     gene            9041..9997
                     /locus_tag="VFN42_12400"
     CDS             9041..9997
                     /locus_tag="VFN42_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HET8997459.1"
                     /translation="MSPRIITLTVNPALDIACSTDQVRHTHKIRTGDDHLDPGGGGIN
                     VARVLHELGGDTLAVLMAGGVTGALVEELLDEAGVPRHTVPIRGRTRICFTVFERSTG
                     LEYRFVPEGPTVTQRDWHAMLNALDTLEGDWIVASGSLEHGMPTDIYAWVARAARRRN
                     QRFVLDTSGPALTAALGSGIELIKPSLGELEALVGRPLPDPAEQEAEALALVRGGAAR
                     LVAVTLGEAGAFVAAPHGVTRIPAVPISVRSAVGAGDSFTAGMTLALARGDTLEEALA
                     WGVAAGTAAVVCAGTARVRKADVEAQYQRLRPGACLPAAPPL"
BASE COUNT         1527 a         3419 c         3467 g         1638 t
ORIGIN      
        1 gccacagcag gaagttggac aggcgctgct cgccggaggt gcggatcacc aggtcgggat
       61 ccggcatgcc ggcggtggtg aggaagccgg acaggcgagc ctcatccagg gtgttggggt
      121 ccagccggcc ggccaccgcc gcctgggcga tcgcgcgcgc cgccgcggcg atctcggccc
      181 gcgcgccgta cgacagcgcc acggtcaggt tgagccgcgt gttggcctcg gttgcgcgtt
      241 ccgcgtcggt caggtcacgc tgaatgtccg gatcgaaccg gccgggctcg ccaatgatgc
      301 gcagccgcac gccattcgcc ttcagctcgg cgatctcggt gcgcaggtaa ttgcgcagca
      361 gaccggtcag gtccagcacc tcggtcaccg ggcggcgcca gttttcgctg ctgaaggcgt
      421 acagggtcag ccaaccgaca ccgctgttga tcgcggcttc gatggtgcgg cgcacggcgc
      481 gggcgccctc ccgatggccg gcgatgcgcg gcagccggcg ggaggcggcc cagcggccgt
      541 tgccgtccat gatgatggcg acgtgcgcag gcactgtcgc ccccgccgtg agggaaccca
      601 gcgacggtgc gatgaggatg tcggtggtgt cgggcatcag taccggatca gacctgtcga
      661 atgtcctttt ccttgtcggc cagcgtctcg tccacctttt tgatgtactg gtccgtgagt
      721 ttctgcacca cgtcgtgcca gtccttggcc acatcctcgc cgatatcgtg cttcttctcg
      781 tagcccttga tctgctccat gccgtcgcgc cgcacgccgc gcacagcgac acgcgcgcct
      841 tcggcgtatt tgtgcgcggt cttcaccagc tcattgcgcc gttccgtggt gagctggggc
      901 agcggcacgc gcacggtctg gccgtcggtg gccgggttca ggcccaatcc ggactcgcgg
      961 atcgcctttt ccaccgcgct gaccagggcg cggtcccaga cctgcacggt gagcatgcgc
     1021 ggctccggcg cgccgaccgt gccgacctgg gtcagcggca tgtcggtgcc gtaggcgtgc
     1081 accttgaccg gttccaacag gccgggatgc gcgcgcccgg tgcgcaggcc gttgaactcc
     1141 cgcttgagcg tctccagtgc gccgtccatc cgccgggtga tgtcctgccg caaggtgtcg
     1201 atgtcgggtg gtgctgccat gggtggttcc cccagtcagg tatcacgaat gtacagttct
     1261 attttggctc gatgatgcgg gtaaaccgtc cctcgccctg catcacggcg gcgaaggcgc
     1321 cgggggcgtg gatgttgaac accacgatcg gcaagccatt ctcccgcgcc aggctgatcg
     1381 ccgccgcgtc catcacgccg agatcgttcg ccagcacgtc gagataggtg agctgctcat
     1441 agcgccgcgc atcggcgacc ttgcgcggat ctgcggaata caccccgtcc acctgggtgc
     1501 ccttcagcag cgcgtcgcag ttcatttcgg cggcacgcag ggcggcggcg gtgtcggtgg
     1561 tgaagaacgg gttgcccgtc ccggcggcga agatcaccac cctgcccttc tccatgtgcc
     1621 gctccgcccg gcggcggata tagggttcgc agaccgagga catcgggatg gcggactgca
     1681 cccgcgtggg aatgccgagc ttttccaggg cggactgcag gcccagcgcg ttcatcacgg
     1741 tcgccagcat gcccatgtaa tcggcctgag cgcgctccat acccgcggcg gcgccggaca
     1801 cgccgcggaa aatgttgccg ccgccgatca ccacgcagac ctgcacgccc atcgccacca
     1861 cgccgccgat atcggtcgcg atggcgcgga ccatgtcgtt gtccaggcca tactcgcgcc
     1921 ggcccatgag ggcctcgccc gagagcttca gcaggacacg tttgtagagc ggggaggaca
     1981 tgcgggcggg ttccggcggt ggcgggggga acctagtggc agggggcgga gggaacaagt
     2041 cagctgggcc ggccggctgg ttcggcactg cagcgggccg cagccattcc gggccagcag
     2101 cggctcgcat cccgggaaat cggaattctg gacagggaac gtggaacacc acgcccagga
     2161 accagtggcg ctgccagttt cggcgaaagt tgcaccttta ggtaggtttc cccgtggcgg
     2221 cacaatcgtg gcggcacaat cgcggcgccg ccgcacgggt gaggtccgga tctggaacaa
     2281 accggctgcc cgccgggagt gcccaccgga agcgccgatc agccgcggac gccgatatgc
     2341 tcccagtcct gcggggtggc cggcagccgc ttgtcggggg tggagcccct ctccagccca
     2401 accgggtgga agcgcgccgc ggaccagccg gccatttcct cgtaggtgcg gtacttctcc
     2461 tccaccgcag cctgggctgc ggccagcagc gtttccgcct cctcgggccg ggtctgcagc
     2521 agcgacttgt agcgaatctc ctgcagcgca tagtcgcgga acggcatggt cgggcgcggg
     2581 ctgtccagca cgaaggggtt ggcgccgtgt gcgcgcatcg ccggattgta gcggaacaat
     2641 ggccagtagc cgctggcgac tgccttctcc tgctgctgca tgccgaactg catgtcgatg
     2701 ccgtgggcga tgcagtgcga ataggccagg atcagcgacg gccccggata ggcctcggcc
     2761 tcgcggaacg cctgcaaggt ctgctgcgga ttggcgccca tcgccacctg ggcgacatag
     2821 acattgccgt aggcgatggc ctgcagcgcc aggtccttgc gcgcggtgcg cttgccggcc
     2881 gcggcgaact tcgccaccgc gccgagcggc gtggctttgg atgcctggcc gccggtgttg
     2941 gaatagacct cggtgtccag caccagcacg ttgacgtcac gcgcgctggc cagcacgtgg
     3001 tccagcccgc cgtaaccgat gtcataggcc cagccgtcac cgccgacgat ccacacgctg
     3061 cggcgcacca gatggtccac caccgacagc aggtcctgcg cggccggggt gccgttgaac
     3121 gtgcgcagcc ggcgggtcag ctccgccacc cgctcgcgct gggcacggat ttccgattcc
     3181 tgcacctgcg gcgcctgcag gatcgcctgc accaggccat cgccaagctg cggcgagagc
     3241 tgctggacca ggcggcgcgc gagttccacg tgctggtcgg cggccaggcg gaagcccagg
     3301 ccgaattccg cattgtcctc gaacagcgag ttgttccagg ccgggccgcg gccctcccgg
     3361 ttggctgacc agggagtgac cggcaggttg ccgccataga tcgaggaaca gccggtggcg
     3421 ttcgccacct gcgcgcgatc gccgaacaat tgcgacagca gtttcaggta cggcgtttcg
     3481 ccacagccgg cacaggcgcc ggaataggcg aacagcggct cgaggaactg cacgccgcgc
     3541 acgttggcga agtccacccg ggcgcggtcg ttcaccggca gccggtcgaa gaagttccga
     3601 ttggcgcgtt cggcctcgat cagcggcgcc ttgtcgcgca tgacgatcgc cttggcgtcc
     3661 gtgccgggcc ggaccgccgg acaggcttcg tggcagagca ggcagccggt gcagtcctcg
     3721 atggagaact gcaaggtgaa gcgcacatcc gggaagccgc gcacgttgat cggcgccgac
     3781 ttgaagccgt ccggcgcacc ctcgagtttc gccgcgtcga aatagcggcc ctggatcacg
     3841 ccatgcgggc agacgaagct gcactggccg cattggatgc acaaatcctg gtcccaggtg
     3901 gcgaccaggt cggacacgtt gcgcttctcc caggcggcgg tgccggaggg ccaggtgccg
     3961 tccaccggca gtttgctgac cggaatctcg tcaccgcggc cttccagcat cagcgcggtg
     4021 acctcacgca cgaagtccgg cgcgttggcg gggaccgccg gctgccgctc ccagttgctg
     4081 ctgacggtgg ccgggacggg cacttcctcc agatggtcca gcgcggcgtc caccgcggcg
     4141 aagttgctgg ccaccacggc ctcgccgcgg cgggcatagg tcttccggat ggccgtcttg
     4201 atgtggtcga gcgcgcgctc gcgcggcagc accccggaca gcgcgaagaa gcaggtctgc
     4261 agcacggtgt tcgcgcggcc acgcagaccg gcgcgctccg ccacgcccga ggcgtcaatc
     4321 acgtacagct tcagcttcag gtcgatgatg cgccgctgca tgtcgcgcgg caggtggtcc
     4381 cacagtgtgt cggcaccata gggcgcgttc agcagcagcg tggcgccggg cgcggccagc
     4441 ttcagcatgt cctggcgggt gacgaactgg aactggtgga tgccgatgaa gctggcccgc
     4501 tcgatcaggt acggcgcatg aatcggctgc gggccgaagc gcagatgcga caccgtctgc
     4561 gcgccggatt tgtgcgagtc gtagacgaag tagccctgcg catgcaggcc agcgtcctcg
     4621 gcgatgatct tcacgctgtt cttgttggca ccgacggtgc cgtcggaacc cagcccatag
     4681 aacagcgccg acaccacgcc cggcggttcc acctggaacg cggcatccgg ctccaggctg
     4741 gtctgcgaca ggtcgtcatg gatgcccagg gtgaagccgt tgcgcggctt gtcgcggttc
     4801 aactcgtcga acaccgcttt ggcgagcggc ggtccaaaat ccttcgacga caggccgtag
     4861 cgtccgccga tgatgctgcg cggcaaacgt ccggcctgcg ccagggcggt gaccacatcc
     4921 agatacagcg gctcgccgag gctgcccggc tccttggtct gctccagcac cgcgatggcg
     4981 cgcacggtag ccggcagttc ggccagcagc gccgccgacg ggaacgggcg gtagagccgc
     5041 acctgcagca cgcccacctt ctcgccgcgc gcgttcagcg caccggcggc gatgcgcgcg
     5101 gtttccgcgc cggtgcccat caccaccact acgcgctccg cgtccggtgc gccggtgtac
     5161 tccatcagcc ggtactgccg gccggtcagc ccggcaaagc gattcatcgc gcgctgcacg
     5221 atctccggca ccgccgcata gtacggattc accgtctcac gcgcctggaa gaacgtgtcg
     5281 gggttatgtg cggtgccgcg gacaaaggga tgctcggggc tcatcgcccg ggcgcgatgc
     5341 gcccgcacca gcgaatcatc gatcatggcg cggatctgcg cgtccgacag cagatcgatg
     5401 gtgccgacct catgcgaggt gcggaagccg tcgaagaagt gcaggaaggg cacgcgcgac
     5461 gcgagcgtcg ctgcctgtgc gatcagcgcg gcatcatgcg cctcctgcac cgaaccggag
     5521 gacagcagcg cgaagccggt ggaacgcacc gccatcacgt cggaatggtc gccgaagatc
     5581 gacagcgcct gcgtcgcgat ggagcgcgcc gcaacgtgga acacggtgga ggtcagctct
     5641 ccggcgatct tgaacatgtt ggggatcatc agcagcagac cctgcgacgc ggtgaacgtc
     5701 gtggtcagcg caccggactg caacgcgccg tgcaccgcac cggcggcgcc gccctcgctt
     5761 tgcatctcct gcacgatcgg caggttgccc cagatgttct tcacgccctg cgccgcccac
     5821 tcgtcggcca gctccgccat ggtggaggat ggggtgatgg ggaaaatggc gcagacttca
     5881 ttcacccgat aggcgacgtg cgccaccgcg gtgttgccgt ccatcgtcgc gggattgcgc
     5941 agcattgggg ggattccttc agacgggggc gggtcgggcg aagggtgtca gattccccct
     6001 ccctttgcgt gagggggtca gggggagggg gcgttcgagg ttacccgggg agctcatgca
     6061 ggccccctcc cccctgcccc ctcccgcaag gggaggggga ggagtcttcg cttccactcc
     6121 cgtgcttcac ggcagttcga caaagccggc ctcggcgacc atttcgatcg cgtgacaggg
     6181 acactgctcg aagcagaccg cgcagccggt gcacaggcgg gtgtccacct ggtagccgtt
     6241 gccgggacca agtttgatga tcgcgccctc gggacaggcc gcaaagcagt tgtcgcattc
     6301 gtagcagacg ccgcaggaca ggcagcggcg agcctcgcgt cgtgcttccg gttcgctgta
     6361 gccgctcagc acttcctcga agccgctgag ccgttcctct tcttccagcc gccgctgcgg
     6421 cgcgggttcc acgtcgctga acaccggcag gtgcagcatt gcgaaattca ccacctccgg
     6481 ccgttgcagc gcgttgtagc ggccaccgcg cagccaggca tcgatgttgc gcgccgccag
     6541 cttgccgtga ccggtggcga ccgtgacggt ccgctcgcac ggcaccagat cgccgccggc
     6601 gaagatgccc ggatggccgg tcatcatgtt gcggtccacc gccaccacgc cgtccggctt
     6661 cacatccacg ccggaaacac tgcgcaggaa cgcgctgtcg ctcacctggc ccaatgccag
     6721 aaccacggcg tcggcttcca gcgtctcgaa ttcgccggtg ggttgcgggc ggcctttgtc
     6781 atccagcgcc atgcgttcga ccgtcagcgt cgggccgacg atctcgcgga tcgtggtcag
     6841 ccacttgatc ttcacgcctt cgtccagcgc ctcgtcggcc tcgaattcgt gcgccggcat
     6901 gtgctggcgg tcgcggcgat acacgatcag cgcctcgctg gcgcccagcc gcttggccgt
     6961 acgtgccgcg tccatcgcgg tgttgccgcc gccatacacc accacgcgcc gcccgagcat
     7021 cggcttgccg ccggtaccgg tctcccgcag catgctgacg gcatcgagca cgcgcaccgc
     7081 atcgcgcgca ggaatgtcga catgctgcgc gacatgcgcg ccgatcgcga cgaacacggc
     7141 gtcgaatttc ccggcgtcac gttcggccag cacgtcggtg accggatggt tcagcacgat
     7201 cttcacgccc atccgctcga tgcgcgccac ctcggcggcc agctcggcgc gcggcaggcg
     7261 ataggcggga atgccgaatt gcagcatgcc gccggccacc gggccggctt catggatttc
     7321 cacctggtgg cccatccgcg tcaggtggta ggcggccgac aggccgctgg gtccggcccc
     7381 gaccaccagc acgcgcttgc cggattgcgg cgcgccgggg ggatagtccc agccctgcgc
     7441 cttcgccatg tcgccgagga agcgttccac cgcgtggatc gacacggcgg aatccaggtg
     7501 gccgcgattg cagccggtct cgcacggatg atagcagacg cggccatgcg tcgccggcag
     7561 cggattgtcc gccaccagcg tttcccatgc cgcgcgatat tcgccggcct gcgccagtgc
     7621 cagccaggcc tggatgttct ctccggcggg acaggcgttg ttgcagggcg gcagcagatc
     7681 gacatacagc ggccgccgcg tgcggaacgg gccggtgcct ttttcgctgg tgaggtcaac
     7741 gaccggtgtc aggtcgcggg cggtcgtgct catggtccgg ttggtcccgt cgttgtttca
     7801 gtcgcggctg agcgcgtccg gctggcgcac aatggcgcgg atcaggtcgg cacgactgac
     7861 gatgccgacg agcttgccct cacgcaggat gggcacgcgc ttgatgtgat ggtgcgacag
     7921 cagatcggcg atttccgtcg cggaactgcg ctcggtcgca gtgatcaccg cgcgttgcat
     7981 gaatttttcc gccggccggt ggtcaaggcg catgtactcc aggaactccg gcgccaggtc
     8041 ggtgccttcg gccagcactt gcagccacca ggcctggcgg tcggccggct tctggccaat
     8101 cgcgggaatc agatcgcctt ccgacaccat gccgagcagg gtgccgtcct tctcgcagat
     8161 ggggatggcg ctgatgccgt tgtcggccat caggcgcgca agctgcgcca cggtcgcgtt
     8221 cggcaatgcg ctgaccacgt tctgggtcat gatctcgctg gcaacgagtg ccatggttcc
     8281 tctcctgtca gaccgcggcg ttcagcctgg ttccgcaacc ggcgcggtgt ccggtgtggc
     8341 ggcgacacgg aatggcggca ggagatcgcc cggcgcggcg ggcacggcag atggttcggc
     8401 cggctgcaat gcgcgggcgg cgacgatatc ccgccaaagc gcgaggatat tcgccgcggt
     8461 ctcctccgcg atgtagcaac gtccttcggc atccacggcg cacagcatcg catcgctgtc
     8521 gtgacgaagc cggcgcagca cgtcggtgat cacttccagg cattccacga cctggtccac
     8581 cgtctgcagc cgcgtgtggc gcaggatgtc cgccaggcgc agcacgcgca tgccttgcac
     8641 cggcacgctt gcagcctccc gccccaattc gaagaacacg ctcaccacgg gcaacagatt
     8701 ctgaagctgc cccagaatgg cctgcagctc ggcgcgcgag cagcggacca gcttcggacc
     8761 cggatgcggc ggctgcactg gcaacggaga gagcagcgcg gcaatctgct gctccagcca
     8821 ctggcgcacc tcgggcggtg cggagcggat ctgttctgcg gacagggtga tgccgaccat
     8881 ggatgagcct cgtgcgctgt ccatgggtga tgcgcccggg ggcatgacgc cgcattgatc
     8941 ctgatcaata tgccgcggac gccgcgccgg ttgacgcagg tcaagctttg ttaaccagcg
     9001 gtcgggcaac atggcggcga tttgcttcgg gacccttgcc atgtcgcccc gcatcatcac
     9061 gctgaccgtc aatcccgccc tggacattgc ctgctccacc gaccaggtgc ggcacacgca
     9121 caagatccgc accggggacg accacctcga tcccggcggc ggcggcatca acgttgcccg
     9181 tgtgctgcac gagctgggcg gcgatacgct ggccgtgctg atggccggcg gcgtgactgg
     9241 cgcgctggtg gaggagctgc tggacgaagc cggcgtgccg cgccacacgg taccgatccg
     9301 cggccggacg cggatctgtt tcacggtgtt cgagcgcagc accggcctgg aataccgctt
     9361 cgtgccggag ggtccgaccg tgacccagcg cgactggcac gcgatgctga atgcgctgga
     9421 cacgttggag ggcgactgga tcgtcgccag cggcagcctg gagcatggca tgccgaccga
     9481 catctatgcc tgggttgccc gcgccgcccg gcggcgcaac cagcgctttg tgctggacac
     9541 ctccggtccc gcgctgacgg ccgcgctggg cagcggcatc gaactgatca agcccagcct
     9601 cggcgagctg gaggccctgg tgggccggcc gctgcccgat ccggccgagc aggaggccga
     9661 ggcgctggcc ctggtgcgcg gtggcgccgc ccggctggtg gcggtcacgc tgggcgaggc
     9721 cggggcgttc gtcgccgcgc cgcatggcgt gacccgcatc ccggcggtac ccatttcggt
     9781 gcgcagcgcg gtcggtgcgg gggacagttt caccgccggg atgaccctgg cgctggcccg
     9841 cggcgacacg ctggaggagg cgttggcctg gggtgtggcg gccggcaccg cggcggtggt
     9901 ctgtgccggg accgcccggg tgcggaaggc ggatgtggag gcgcaatacc agcgcctgcg
     9961 ccccggtgct tgcctcccgg cagccccgcc gctatagccg cccgctcagt ctgcgcgccg
    10021 ggcctggaat gggcatgccc ggccgcgact g
//
LOCUS       DASSZH010000571         4373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1428267, whole genome shotgun sequence.
ACCESSION   DASSZH010000571 DASSZH010000000
VERSION     DASSZH010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428267"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VFN42_12405"
     CDS             complement(<1..525)
                     /locus_tag="VFN42_12405"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8997460.1"
                     /translation="MEFGMFHEFQRMPGQTEAEAFAVSFEQVDTAERSGLDVMWLAEL
                     HTAPERSVLAAPLTIASAIAARTQRMKIGIAVQVLPLCHPLRLAEEACTVDHISHGRL
                     IMGVGRSGFPRVYQAYGISYAESRERFAEVLEILRRSWTEERFSFHGKYYNFDNVYLV
                     PKPYTQPYPELRIAA"
     gene            675..1820
                     /locus_tag="VFN42_12410"
     CDS             675..1820
                     /locus_tag="VFN42_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET8997461.1"
                     /translation="MSDDPAGLSPLLGAIYDAPLEPAQWEETIAQLRTALGGAAAAFF
                     HFDSAVPGRTNATILDGYASSSAAEYTQHFGGRDIRMPHVLRLESAGVYADDRQMPFA
                     DVARSEIFQDFYRPLGLGHGIATKLFGDQTRISVLSVHRPRRGSGFDPKAVALLETVA
                     PHLVRALQLQRQVERARSLAHGLAAGLDHFHMPVFLVDRGARVLELNSAATDLLRRAD
                     SPLRCVQGQLATIAPRLTARLHESVRSASGVLAGEPVRPPAILQLATADDDRRCSVMV
                     APVRRGDRLGLRAAPLALVFVSVPRLKPVLDSDGLIRMFGLSAAEASIALGLMEGDSP
                     AEIADRRQVAQETVRSQVKSVLAKTGTRRQGELIALLSRSLAALRLR"
     gene            1889..3148
                     /locus_tag="VFN42_12415"
     CDS             1889..3148
                     /locus_tag="VFN42_12415"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HET8997462.1"
                     /translation="MHIDSEPTQHLQHAAQAARAASYVLARSLTRQRNDGLRAMAAAL
                     RAAEPQILEANAIDLAHSTGTAAFRDRLTLTPARVEAMAKGLEDVAALPDPLGRVLAD
                     WTRPNGLRIQRVATPIGVIGMIYESRPNVGADAAALCLKSGNAVILRGGSESLHSAQA
                     IHAALTAGLRQAGLPEACVQIPPSADRAYVAAMLAAAGLIDLIIPRGGKSLVERVQRE
                     ARVPVLAHAEGLCHTYVHAAADPDMARRIVANAKMRRTGICGATETLLIDRAVAPKLL
                     PPIIAELRALGCDFRADAASRDIVPDLPAASEADFDTEWLDAVLSVAVVDGVDAALAH
                     ITRHGSEHTDAIVTEDAAVAEYFLAGLNSAVGLWNASTQFCDGGEFGFGAEIGIATGR
                     IHARGPVGLEQLTTYRYVVRGTGQVRP"
     gene            3157..3771
                     /locus_tag="VFN42_12420"
     CDS             3157..3771
                     /locus_tag="VFN42_12420"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HET8997463.1"
                     /translation="MAFDPIPRFGDRRRTRVGLLGGSFNPAHAGHRQIAALARKRLRL
                     DQVWLLVSPGNPLKPQRGMAPLPQRLAGAQAIADGRRTVATAIEAKLGTRYTVDTLRL
                     LLQRFPRVRFVWLMGADILTQLPQWRRWRDILCRIGFAVLPRPGYNLAALKGQAAQRL
                     RRCRLRERAAVLVPGNAPCWVFLHVPQTAISATAIRAAHKGTVP"
     gene            3840..4277
                     /gene="rsfS"
                     /locus_tag="VFN42_12425"
     CDS             3840..4277
                     /gene="rsfS"
                     /locus_tag="VFN42_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET8997464.1"
                     /translation="MPVADKTPGTPRKKTIVAGPKAATPRPRRKVEPSFLDKLQSVIV
                     GSLEDDKAENVVTLDLEGKATFCDRMVIATGLADRQITAMAQHLTEKLHEAGFKRVQV
                     EGAGGSEWVLIDAGDIVVHLFKPEARTTYGLEKMWGADLDEAG"
BASE COUNT          672 a         1558 c         1506 g          637 t
ORIGIN      
        1 cgcggcgatg cgcagctcgg gatagggctg ggtgtacggc ttcggcacca gatagacgtt
       61 gtcgaagttg tagtacttgc cgtggaagga gaagcgctcc tcggtccacg accggcgcag
      121 gatctccagc acctcggcga agcgttcgcg gctttccgca taggaaatgc cataggcctg
      181 gtacacccgc ggaaagccgc tgcgcccgac gcccatgatc agccgcccat ggctgatatg
      241 gtccaccgtg caggcctcct ccgccaggcg gagcggatgg cacagcggca gcacctgcac
      301 cgcgatgccg atcttcatcc gctgggtacg cgcggcgatg gcgctggcga tggtcagcgg
      361 cgcggcaagc accgaccgct ccggcgcggt atgcagttcc gccagccaca tcacgtcgag
      421 acccgagcgc tccgcggtat ccacctgctc gaacgacacg gcaaaggctt ccgcctcggt
      481 ctggcccggc atgcgctgaa actcatggaa cataccgaat tccatcggcg tacctcccat
      541 tcattggcaa cgagcgttga ccggtttccc agcgcgcgca agtccggcca caggccggcg
      601 cgcgtgacaa acgtttcgtt tccgggcagg gttttgccag gcttcgccaa aggccaggga
      661 caggggacca gagcgtgagt gacgatcccg cggggctgtc gcccctgctc ggcgccatct
      721 acgacgcgcc gctcgagccc gcacaatggg aggagactat cgcgcagctc cgcacggcgc
      781 tgggcggcgc ggccgccgcc ttcttccatt tcgacagtgc ggtccccggc cggaccaacg
      841 cgaccatcct ggacggctac gcgtccagct cagccgccga atacacgcag catttcggtg
      901 gacgcgatat ccgcatgccg catgtcctgc ggctggagtc cgcgggcgtc tatgccgatg
      961 accggcaaat gcccttcgcc gatgtggccc gaagcgagat cttccaggac ttctaccgtc
     1021 cgctgggcct cggtcacggc atcgccacca agctgttcgg cgatcagacc cggatcagcg
     1081 tgctcagcgt ccaccgcccg cggcgcggca gtgggttcga tcccaaggcc gttgcgctgc
     1141 tggagacggt cgcaccgcac ctcgtccgcg ccctccagtt gcagcgccag gtcgagcgcg
     1201 cgcgcagcct ggcgcacggt ctcgcggccg ggctggacca cttccacatg ccggtgttcc
     1261 tggtggaccg cggcgcgcgc gtgctggagt tgaacagcgc cgcgaccgat ctgctccgcc
     1321 gagcggacag cccgttgcgc tgcgtccagg ggcaactggc cacgatcgcg ccacgcctca
     1381 ccgcccggct gcacgaaagc gtccggtctg cgtcgggcgt gttggccggc gagccggttc
     1441 gcccaccggc catcctgcag cttgcaacgg cggacgacga ccgccgttgc agcgtgatgg
     1501 tcgcaccggt ccggcgtggc gatcggctcg gcttgcgcgc ggcgccgctc gccttggtgt
     1561 ttgtctccgt ccctcgcctg aagccggtgc tggacagcga cgggctgatc cggatgttcg
     1621 ggctgagtgc cgccgaagcc agcatcgcgc ttggcctgat ggaaggcgac agtccggccg
     1681 agatcgccga tcgccgccag gtggcccagg agacggtgcg atcccaggtg aagagcgtgc
     1741 tggcgaagac cggcacgcgg cggcagggcg aattgatcgc gctgctgtcg cgcagcctgg
     1801 ccgcgctgcg gctgcgctag agcgaggcgt gcggggccca ctggaaaccg cgcgccatga
     1861 cgggcaaact ctccccaagg agcgcgccat gcatatcgac agcgaaccca cccagcatct
     1921 gcagcatgcg gcacaggccg cgcgtgcggc atcgtacgtg ctggcgcgca gcctcacgcg
     1981 gcagcgcaat gacgggctgc gcgcgatggc cgccgcgctg cgcgccgctg agccgcaaat
     2041 cctggaagcc aacgccatcg acctggcgca cagcaccggc accgcggcct tccgcgaccg
     2101 gctgacgctg acgccggcac gcgtcgaggc catggccaag ggcctggaag acgtcgccgc
     2161 cctgcccgat ccgctcggcc gcgtcctcgc cgactggacc cggccgaacg ggctgcgcat
     2221 ccagcgcgtg gccacgccga tcggcgtgat cggcatgatc tacgagagcc gccccaatgt
     2281 cggcgccgac gccgccgcgc tgtgcctgaa gtccggcaac gcggtgatcc tgcgcggcgg
     2341 atcggaaagc ctgcacagcg cccaggcgat ccatgccgcc ctcaccgccg gcctgcgcca
     2401 ggccggcttg ccggaagcct gcgtgcagat cccgcccagc gccgaccgcg cctatgtcgc
     2461 ggcgatgctg gccgcggccg gcctgatcga tctgatcatc ccgcgcggcg gcaagtcgct
     2521 ggtggaacgc gtgcagcgtg aggcccgggt gcccgtgctg gcgcatgccg agggcctctg
     2581 ccacacctat gtgcacgcag ccgccgatcc ggacatggcg cggcggatcg tcgcgaatgc
     2641 gaagatgcgc cgcaccggca tctgcggcgc caccgaaacc ctgctcatcg atcgcgccgt
     2701 ggcgcccaaa ttgctgccgc cgatcatcgc cgaactgcgt gcgctgggct gcgatttccg
     2761 cgccgatgcc gcatcgcggg atatcgttcc cgacctgccg gccgcgtcgg aggccgattt
     2821 cgacaccgag tggctggacg cggtgctgtc ggtcgcggtg gtagacgggg tggatgccgc
     2881 gctggcgcat attacccggc acggcagcga acacaccgat gccatcgtca ccgaggatgc
     2941 ggcggtggcc gaatacttcc tcgccgggct gaacagcgcg gtggggctgt ggaacgcctc
     3001 cacccagttc tgcgacggcg gtgagttcgg tttcggcgcc gagatcggca tcgccaccgg
     3061 ccgcatccac gcgcgcggcc cggtcgggct ggagcagttg accacctacc gctacgtggt
     3121 gcgcggcacc gggcaggtcc ggccctgaac gcctcgctgg ccttcgatcc catcccccgc
     3181 ttcggcgacc gccgccgcac gcgcgtcggg ctgctcggcg gctcgttcaa tccggcgcat
     3241 gccgggcacc ggcagatcgc cgcgctggcg cgcaagcggc tgcggctgga ccaggtgtgg
     3301 ctgctggtgt cgcccggcaa tccgctgaag ccgcagcgcg gcatggcgcc gctaccgcaa
     3361 cgcctggccg gcgcgcaggc catcgccgac gggcggcgca ccgtcgccac cgcgatcgag
     3421 gccaagctgg gcacccgcta caccgtcgat acgctgcgcc tgctgctgca gcgcttcccc
     3481 cgcgtgcgct tcgtctggct gatgggcgcc gatatcctga cgcaactgcc gcaatggcgg
     3541 cgctggcggg atatcctttg ccgcatcggc ttcgcggtgc tgccgcggcc cggctacaac
     3601 ctggcggcgc tgaagggcca ggccgcgcag cgcctgcgcc ggtgtcgtct gcgtgaacgc
     3661 gccgcggtgc ttgtgccggg gaatgcgccg tgttgggtct tcctgcacgt gccgcagacg
     3721 gctatctccg ccactgcgat ccgcgcagcc cacaaaggaa ccgtgccata gccaagcccc
     3781 cgccaccgcc gtcgcctgcc cggcacaagc cggcaccgcg caagcgggtg aagccgccca
     3841 tgccggtggc cgacaagacc cccggcacgc cgcgcaagaa gaccatcgtc gccgggccga
     3901 aagccgcgac accgcgccca cgccgcaagg tggagccatc gtttctggac aagctgcagt
     3961 cggtgatcgt cggcagcctc gaggacgaca aggccgagaa cgtcgtgacc ctggacctgg
     4021 aggggaaggc gacgttctgc gaccgcatgg tgatcgccac cggcctggcc gaccggcaga
     4081 tcaccgcgat ggcgcagcac ctgaccgaga agctgcatga ggccgggttc aagcgcgtgc
     4141 aggtggaagg cgccggcgga tcggaatggg tactgatcga tgccggcgac atcgtggtgc
     4201 acctgttcaa gccggaagcg cgcacgacgt acgggctgga gaagatgtgg ggcgccgacc
     4261 tcgacgaggc cggctgatgg cggccggcgc cggtcggcag caggtcgtca cggcgtatcg
     4321 cgaactggcg cggcacggca tgaatgcggg cagttcaggc aatgtcagcc tgc
//
LOCUS       DASSZH010000572         4782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_814608, whole genome shotgun sequence.
ACCESSION   DASSZH010000572 DASSZH010000000
VERSION     DASSZH010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814608"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VFN42_12430"
     CDS             <1..520
                     /locus_tag="VFN42_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019089737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HET8997465.1"
                     /translation="TDAARQLQARVALMRRIEPEAGWPDLSDATLTGSVRDWLAPHLL
                     GLSRLADLDRLDLPAILRGRLSWELATRLDRALPTQLGLPAGRAPVDYTTPVPVAAAR
                     AQAFYGLTETPRLAEGRIPLQLALLSPAGRPVAVTADLAGFWQRGWADVRRDMRGRYP
                     RHAWPENPAVPA"
     gene            631..2883
                     /locus_tag="VFN42_12435"
     CDS             631..2883
                     /locus_tag="VFN42_12435"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8997466.1"
                     /translation="MSLRLRMILLAGVCILPSVLLLASTQWQLRQARAAEVRREIAEL
                     ASSEANEIATIIGGARQFLGALEHSPAVLQRNAPLCSAWLARLRHDNPIYASIRADDL
                     SGRAFCSSDPGRAAFDGDEPYFQEVLRRRGFAVGTEAVSPLTHEPELPLARIVVDDEG
                     SPADVLVVELDLSWLQQDLAARLPPDASLTLLDRNGHALLHAAGSRQPPTGSPDQIVS
                     RAQVGDGGTGLEVIAARPRLSAFAALDRTTRDGMVFIVLGLLLACLGAAWIGRRFIQA
                     PINALLTATERLRNGDYTARAHPAADASELAQLGSGLNALAGDLEQRKRAQADAEARL
                     RQLAATLEQRVELRTRELAEANERLAAEAEQRQRTQAELAQMQKLDAIGKLTGGIAHD
                     FNNLLAAVLGSLEMVLPRVDDARLKRLLNVATQAAQRGAKLTSHLLAFSRKQDLVLRP
                     VDVNAVITGMDDLLSRTLGKLVRLDFDLSDELWPATGDPVQLEVGLLNLAINARDAMP
                     EGGTLAFRTRNVHVAEGPHAGVTLPSGDYAMVAVIDSGEGMPDSVLAKVFEPFFTTKG
                     PGKGTGLGLSMIYGFARQAGGTVTIDSTPGKGTAVSLYLPRAREQAEADPVRPAEAQS
                     ASRRLHVLLVDDDSMVRETTREMLLMSGHTVVEAATPEAALALLRDGEQFNLLVADFS
                     MPGMTGVQLAERAHELRPQLPVLIITGFAEADGLQPTARVWPVLRKPFYAADLADAVR
                     ASVAASSAPD"
     gene            2969..3823
                     /locus_tag="VFN42_12440"
     CDS             2969..3823
                     /locus_tag="VFN42_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET8997467.1"
                     /translation="MAFKDILVHLDSGVRCKTRLTAALGLARRFQSHLTGTYVLDIPA
                     PEFFYGAAMPFMGAGPEQVIERMRLDAINTAAPVEQGFRDSLNREGVPGDWRLVEGSL
                     PATVALHARYADLAVVAQPNPYEPNDGGQRDIAVTVVMTSGRPVLVVPFAGEFPTIGE
                     RVLVAWNATREAARAVNDALPLLAQAKLVTVLAVNPKHGINGHGDVPAADIALHLARH
                     GVRAEAAHTVARDIPEGEALLSYAADIGADLIVAGAYGHSRAREVVFGGVTRTLLNEM
                     TVPAFLSH"
     gene            3872..>4782
                     /locus_tag="VFN42_12445"
     CDS             3872..>4782
                     /locus_tag="VFN42_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997468.1"
                     /translation="MAAHPPLGEDAMRTLPAAGAEPVALDPDRQLRAALPVLAALIGY
                     AVFLFAPPVLNDGDTWWQLAAGQWILHHRAIPFADPFSYTFAGTPWVAQEWLSEVFTA
                     LAFRLGGWDGVLMLYGTAAGLTFGLLALHLRRWTDGLCLVLLLALGAACTSGSLLVRP
                     HLLALPVLELWTAGLLIARSRGAAPSLLLLPLMALWANLHGSFMLGLLLPLPLALEAA
                     MDAPPDRGTVLRGWAVFIAGAWLAALVTPQFWHGLVFPFRLLAMHDLAAITEWRPTNF
                     ATLQPIEIALAVLLYVTWSRGIRLPLLR"
BASE COUNT          669 a         1663 c         1702 g          748 t
ORIGIN      
        1 gaccgacgcc gcgcgccagc tgcaggcgcg ggtcgcgctg atgcgccgga tcgagccgga
       61 agccggctgg cccgatctgt cggatgcaac cctgacagga agcgtccgcg actggctcgc
      121 gccgcatctg ctggggctga gccggctggc cgatctcgac cggctcgacc ttcccgccat
      181 tctgcgcggc cggctgagct gggagcttgc cacccggctg gatcgcgccc tgccgacgca
      241 actcggcctg ccggcgggac gcgccccggt cgattacacc acgccggtgc cggtggccgc
      301 cgcccgggca caggcctttt acggccttac cgaaacgcca cgcctcgccg aggggcggat
      361 tcccctgcaa ctggcgctgc tgtcgccagc cgggcggccg gtggcggtca ccgccgacct
      421 cgccggattc tggcagcgcg gctgggccga cgtacgccgg gacatgcggg gccgctatcc
      481 caggcacgcc tggccggaaa acccagccgt tccggcataa ccgaccagaa tcccaataat
      541 tccccccggg cctcgggtcg gcggcttaaa tcggggtatg ctcacagttc ggtgatgtag
      601 cgtgatcggc atcggtgcta tccaaacgcc atgagtctgc gtctccgcat gattctcctt
      661 gccggcgtct gtatcctgcc ctcggtgctg ctgctcgcca gcacccagtg gcagttgcgc
      721 caggcgcgtg cggcggaagt ccggcgggag atcgccgaac tcgccagcag cgaggccaac
      781 gagatcgcca cgatcatcgg gggcgcccgg cagttcctcg gcgcgctgga gcacagcccc
      841 gcggtgctgc agcgcaacgc cccgctgtgt tccgcctggc tggcccggct gcggcatgac
      901 aacccgatct atgccagcat ccgcgctgac gatctttccg gccgcgcctt ctgttccagc
      961 gaccccggcc gagccgcgtt cgacggcgac gagccgtatt tccaggaggt gctgcgccgg
     1021 cgcggctttg ccgtggggac ggaagccgtc tccccactga cccatgagcc cgaactgccg
     1081 ctggcgcgca tcgtggttga tgacgaaggc agtccggccg acgtcctggt ggtggagctc
     1141 gacctgtcct ggctgcaaca ggacctcgcc gccaggctgc cgccggatgc gtcgctgacc
     1201 ctgctcgacc ggaatgggca tgcgctgctg cacgccgcgg ggtcgcgcca accgccgacc
     1261 gggtcgccgg accagatcgt gagtcgcgcc caggtgggcg acggcgggac cgggctcgaa
     1321 gtgatcgcgg cgcggccgcg gctgtcggcc ttcgccgcgc tggaccgcac cacccgagac
     1381 ggcatggtgt tcatcgtgct ggggctgttg ctggcctgcc tgggcgctgc ctggatcggc
     1441 cgccgcttca tccaggcgcc gatcaacgcg ctgctgaccg ccaccgagcg tctgcgcaac
     1501 ggtgactaca ccgcgcgcgc ccatcctgcc gccgatgctt cggaactggc gcagctcggc
     1561 agcggcctga acgcgctggc cggcgatctg gaacaacgca agcgggctca ggccgacgcc
     1621 gaggcccggc tgcgccagct tgccgcgacg ctggagcagc gcgtggaact gcgcacgcgc
     1681 gagctggccg aggcgaacga gcgcctggca gcggaagccg aacagcgcca gcgcacccag
     1741 gccgaattgg cgcagatgca gaagctggac gcgatcggca agctgaccgg cggcattgcg
     1801 cacgacttca acaacctgct ggcggcggtg ctggggtcgc tggagatggt gctgccgcgg
     1861 gtggacgacg cccggctgaa acggctgctg aatgtggcga cgcaggcggc gcagcgcggg
     1921 gcaaagctga cctctcacct gctggcattt tcgcgcaaac aggacctggt gctgcgcccg
     1981 gtggacgtga atgccgtgat caccgggatg gacgatctgc tcagccgcac gctcggcaag
     2041 ctggtgcggc tggacttcga cctgtcggac gagctgtggc cggcgaccgg cgatccggtc
     2101 caacttgaag tcgggctgct gaacctggcg atcaatgcgc gcgacgctat gccggagggc
     2161 ggcacgctgg ccttccgcac ccgcaacgtg catgtcgcgg aggggccgca tgccggggtc
     2221 acgctgccaa gcggggacta tgccatggtg gcggtcatcg attccggcga aggaatgcca
     2281 gactcggtgc tggccaaggt gttcgagccg ttcttcacga cgaagggccc cggcaagggc
     2341 accggcctgg gcctgtccat gatctacggc ttcgcccgcc aggccggcgg caccgtcacc
     2401 atcgacagca cgccgggcaa ggggaccgcg gtgagcctgt atctgccgcg cgcccgcgag
     2461 caggccgaag ccgatccggt gcggcccgcc gaagcgcaat cggccagccg gcggctgcat
     2521 gtcctgctgg tcgatgacga ttcgatggtg cgggagacca cgcgggagat gctgctgatg
     2581 tccggccaca ccgtggtcga ggcggcgaca ccggaagcgg cgctggcgct gctgcgcgac
     2641 ggggaacagt tcaacctgct ggtggccgac ttctccatgc cgggcatgac cggcgtgcag
     2701 ttggcggagc gcgcgcacga attgcggccg caattgccgg tgctgatcat caccgggttc
     2761 gccgaggcgg atgggctgca accgaccgcc cgcgtctggc cggtgctgcg caagccgttc
     2821 tacgcggccg acctggcgga tgcggtacgc gccagcgtgg cagcatcgag cgccccggac
     2881 tgacgcccgg cagcgagtga tccatatcaa cgcatcccgc gggatgcgat tgcagagtgc
     2941 cgccagaaca ttgcaatgga ggcacaccgt ggctttcaag gacattctgg tgcatctgga
     3001 cagtggcgtc cggtgcaaga cccgcctgac cgctgccctg ggactggccc gccgtttcca
     3061 gtcgcatctc actggcactt acgtgttgga catccccgcg cccgagttct tctacggcgc
     3121 ggcgatgccc ttcatgggcg ccgggccgga gcaggtgatc gagcggatgc ggctcgatgc
     3181 catcaacacc gcggccccgg tcgagcaagg cttccgtgac agcctgaacc gcgagggagt
     3241 gccaggcgat tggcgcctgg tggagggcag cctgcccgct acggtggcgc tgcacgcacg
     3301 ctatgccgac ctggcagtgg tggcgcagcc caatccctat gaaccgaacg acggcgggca
     3361 gcgcgacatc gcggtgaccg tggtgatgac ctccggccgg ccggtgctgg tggtgccctt
     3421 tgccggcgag ttccccacca ttggcgagcg cgtgctggtg gcctggaatg ccacgcgcga
     3481 agccgcccgg gcggtgaacg acgcgctgcc gctgctggcg caggccaagc tggtgaccgt
     3541 gctggcggtg aacccgaagc atggcatcaa cggccatggc gacgtgccgg cggccgatat
     3601 cgcgctgcac ctggcacgcc acggcgtgcg ggccgaggcc gcccacaccg tcgccaggga
     3661 tatcccggag ggcgaggcgt tgctgtccta tgccgcggat atcggcgcgg acctgatcgt
     3721 ggccggcgcg tatggccatt cgcgtgcgcg ggaggtggtg ttcggcggcg tcacccggac
     3781 gctgctgaac gagatgacag tgcccgcctt cctgtcgcac tgaccggctc gtcctcgctg
     3841 aaacgccggc gcaaggccgg caggcacaca gttggcggcc catccaccgc tcggcgagga
     3901 cgcgatgcgt acgctgccgg cggccggcgc agagccggtc gccctcgatc ccgatcggca
     3961 actgcgcgcc gcgctgccgg tgctcgccgc gctgatcggc tacgccgtgt tcctgttcgc
     4021 cccgccggtg ctgaatgacg gcgacacctg gtggcagctc gccgccgggc aatggattct
     4081 gcaccaccgc gccatcccct tcgccgaccc gttctcctac accttcgccg gcacaccctg
     4141 ggtggcacag gaatggctgt cggaggtgtt caccgccctc gccttccggc tgggcggctg
     4201 ggatggcgtg ctgatgctgt atggcacggc ggcggggctg accttcggcc tgctggcgct
     4261 gcatctccgc cgatggacgg acgggctgtg cctggtgctg ctgctggcgc tgggcgccgc
     4321 ctgcacgtcc ggcagcctgc tggtgcggcc gcatctgctg gcgctgccgg tgctggagct
     4381 gtggaccgcc ggcctgctga tcgcgcgcag ccgtggcgcc gcgccgtccc tgctgttgct
     4441 gccgctgatg gcgctgtggg ccaacctgca cggcagcttc atgctggggc tgctgctgcc
     4501 gctgccgctg gcgctggaag cagcgatgga cgccccgccg gaccgcggga cggtgctgcg
     4561 cggctgggcg gtgttcatcg ccggcgcctg gctggcggcg ctggtcaccc cgcagttctg
     4621 gcacgggctg gtcttcccgt tccgcctgct ggcgatgcac gacctggccg ccatcaccga
     4681 gtggcggccc accaatttcg cgacgctgca gcccatcgag atcgcgctgg cggtgctgct
     4741 ctacgtgacg tggtcgcgcg gcatccgcct gccgctgctg cg
//
LOCUS       DASSZH010000573         2352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_593443, whole genome shotgun sequence.
ACCESSION   DASSZH010000573 DASSZH010000000
VERSION     DASSZH010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593443"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VFN42_12450"
     CDS             complement(<1..614)
                     /locus_tag="VFN42_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET8997469.1"
                     /translation="MRSNLGPDIDLATRLFTALRDATFDGVGITREAYGPGEQKAHAL
                     VRSEAEALGLEIANDAAGNLYMTLPGRDRAAKRIVLGSHLDSVPQGGNYDGAAGVLAG
                     LATVAGMRRAGFMPSCDITVLAVRAEEAGAWFPTSFPGSHAALGRLPPEALSLRRMDT
                     GRTLEDHMRELGFDPDAVRAGKRQLGPHNTAAYLELHIEQGPVL"
     gene            711..1679
                     /locus_tag="VFN42_12455"
     CDS             711..1679
                     /locus_tag="VFN42_12455"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HET8997470.1"
                     /translation="MKPKLVVAFLTTRDVVMRARDEFDAVVVDGDNDMTVPEVVQAAA
                     EHRAEAVLFTNTLPLSAQAIAALPPSVRVGATSSVGYDHIDVGAAKARGLVVTNTPDV
                     LTECTADHAFMMLLAAARRGYEYDQIMRSGWRYRIGQGDLLGVRVTGKTLGIFGMGRI
                     GRAMAQRARGFDMRVVYHARRRLPADLEAGARYFQHFHDMLPHCDFLSLHAPATPVTD
                     RIIDARALALLPRGAVLVNVSRGGLVDEDALFQALTSGQLFAAGLDVFRSEPDYDLRF
                     GKLPNVILSPHVGSATVETRNAMGFRALDNIAAVLSGRGPIDPLWH"
     gene            1690..>2352
                     /locus_tag="VFN42_12460"
     CDS             1690..>2352
                     /locus_tag="VFN42_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8997471.1"
                     /translation="MPKPVLLITRRLPPAVEARAGAEFDALLTESDTPVPDVLTRADG
                     ADAVLCCPGDRLDAAAIAALPGSVKVIGTFSVGYDHIDVPAAKARGIAVCNTPDVLSV
                     ATAECAMLLILAAARRAGEGERMVRSGQWQGWGPLQLLGTQVSGRKLGIFGMGRIGRE
                     LARIARGFQMQVHYRDMVRLPEHMESGAVFHDSDASFLPECEVLSLHAPGGESTQHWL
                     NAA"
BASE COUNT          354 a          821 c          809 g          368 t
ORIGIN      
        1 tccaacaccg gcccctgttc gatgtgcagt tccaggtacg cggcagtgtt gtgcggccca
       61 agctgccgtt tgccggcgcg caccgcatcc gggtcgaagc ccagttcccg catgtggtcc
      121 tccagcgtgc ggccggtatc catccggcgc agcgacagcg cctccggcgg caggcgaccc
      181 aacgccgcat ggctgccggg aaacgaggtg gggaaccagg caccggcctc ttcggcacgc
      241 accgccagaa cggtgatatc gcatgacggc atgaaaccgg cgcgccgcat ccccgccacc
      301 gtcgccagcc cggcgaggac gcccgcggcg ccgtcgtaat tgccgccctg cggcacggaa
      361 tccaggtggc tgccaagcac gatccgcttc gccgcgcggt cgcggccggg cagcgtcata
      421 tacagattgc cggccgcgtc gttggcgatc tccaggccca gggcttcggc ttcgctacga
      481 accagggcat gcgccttctg ctcgcccggg ccgtacgcct cccgcgtgat ccccacgccg
      541 tcgaaagtgg catctcgcaa agccgtgaac aggcgggtgg cgagatcgat gtccgggcca
      601 agattgctgc gcatgtcggg tctccgtgca ggccgcgcac cgtgccgtgc ggctgcccct
      661 ttcgcaaccg cctgggtctg ggccataacc gccaggcaga ggagtccggc atgaaaccaa
      721 agctggtggt ggcattcctc accacgcgcg acgtggtgat gcgtgcccgc gatgaattcg
      781 acgccgtcgt cgtggacggc gacaacgaca tgacggtgcc ggaggtggtg caggccgcgg
      841 cagagcaccg cgccgaggcc gtgctgttca ccaacacgct gccactatcg gcgcaggcca
      901 tcgccgcgct gccgcctagc gtgagggtcg gcgcgaccag cagcgtcggc tacgaccaca
      961 tcgatgtcgg cgcggcgaaa gcgcgcggcc tggtggtgac caacacgccg gatgtgctga
     1021 ccgaatgcac cgccgaccat gcattcatga tgctgctggc tgccgcccgc cgcggctacg
     1081 aatacgatca gatcatgcgc agcggctggc gctatcgcat cggccagggt gaccttctgg
     1141 gcgtgcgggt caccggcaag acgctcggaa tcttcggcat gggccgcatc ggccgcgcca
     1201 tggcgcagcg ggcgcgcggc ttcgacatgc gggtggtgta tcacgcccgc cgccgccttc
     1261 ccgccgacct ggaagccggc gcgcggtatt tccagcactt ccacgacatg ctgccgcact
     1321 gcgatttcct ctcgctgcat gcgcccgcca caccggtgac cgaccgcatc atcgacgcgc
     1381 gggcgcttgc gctgctgccc cgcggcgccg tgctggtgaa cgtctcgcgc ggcgggctgg
     1441 tggacgagga cgcgttgttc caggcactga cctccggcca attgttcgcc gcggggctcg
     1501 atgtgttccg cagcgagccg gattacgatt tgcgctttgg caagctgccc aacgtgatcc
     1561 tgtcgccgca cgtcggcagc gcaaccgtcg agacacgcaa cgccatgggt ttccgcgccc
     1621 tggacaacat cgccgcggtg ctgtccggcc ggggcccgat cgaccctctg tggcactgaa
     1681 aggccgccaa tgccaaagcc cgttctgctg atcacccgcc gtcttccgcc ggccgtcgaa
     1741 gcccgcgccg gcgccgaatt cgacgctctg ctgaccgaat ccgacacgcc ggtccccgac
     1801 gtgctgacgc gcgccgacgg ggcggatgcg gtgctgtgct gcccgggcga ccggctggat
     1861 gcggcggcga tcgccgcgct gccgggctcg gtgaaggtga tcggcacgtt cagcgtgggc
     1921 tacgaccata tcgacgtgcc cgcggcgaag gcacgcggca tcgcggtgtg caacacgccg
     1981 gatgtgctga gcgtcgcgac ggcggaatgc gccatgctgc tgatcctggc ggccgcgcgc
     2041 cgagccggcg aaggcgagcg catggtgcgc tcgggccagt ggcagggctg gggaccgctg
     2101 caattgctgg gcacgcaggt ctcgggccgc aagctcggga ttttcggcat gggccgcatc
     2161 gggcgcgaac tcgcgcgtat tgcacgcggc ttccagatgc aggtgcatta tcgcgacatg
     2221 gtccgcctgc cggaacacat ggagtccggc gcggtgttcc acgacagcga cgccagcttc
     2281 ctgccggagt gcgaggtgct gtcgctgcat gctccgggcg gcgagtccac gcagcactgg
     2341 ctgaacgcgg cg
//
LOCUS       DASSZH010000574         2709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1343205, whole genome shotgun sequence.
ACCESSION   DASSZH010000574 DASSZH010000000
VERSION     DASSZH010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343205"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1798
                     /locus_tag="VFN42_12465"
     CDS             <1..1798
                     /locus_tag="VFN42_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HET8997472.1"
                     /translation="VMFTAGFAEMDEAGEAAQHRMVATARQHGMRLLGPNCLGVFDAR
                     TAYYATFSSSFDSGWPVMGRIGIASQSGAYGTHLYTLARNRGIGASLCIMTGNEADVT
                     VGECIGWLAENPEVDVIAAYIEGIRESASLIAAFEAARAARKPIIMQKVGRSELGSKA
                     AKSHTASIAGDDAVTEAVMREFGVARARNSEEMLDIAHTATRKIYPARNSLGVITVSG
                     GAGVLISDTADSLGLAMPEMPADTQKFLRELVPFCAPRNPVDATAQVSNDVKLINTFT
                     EAMVRDGGYASILGFFSMTASSRRWPAIREQLNLVKAQYPDRLYALSVIVPPERRDEL
                     EADGWVVHEDPTRAVVAIDAMGRFGAAFAAPAGEKPPAVHAITLPDSTPSEAEAKRLL
                     ASAGITSAPEQACGSADEAAAAAELFGFPVVMKILSPDILHKSEIGGVLLGVADAAAV
                     RDGYDLLLARAKRAAPHARIEGVLVAKQLQGGVECILGIHRDPVFGPIAMFGLGGIFV
                     EVMKDVVFRRCPFGADVAEQMIRSIKGAPLLLGARGRPPVDVHALAQMLSRLSAFAVA
                     AGPRLVSVDLNPVFAMPAGHGAFAVDAVVEVA"
     gene            2316..2471
                     /locus_tag="VFN42_12470"
     CDS             2316..2471
                     /locus_tag="VFN42_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997473.1"
                     /translation="MSDAAAEMAEPGFQPSPAQVASVILSVAVRRLGPDLKRDATQAL
                     KAASGST"
BASE COUNT          442 a          867 c          957 g          443 t
ORIGIN      
        1 ggtgatgttc accgccggct tcgcggaaat ggatgaggcc ggcgaggcag cccagcaccg
       61 catggtcgcc actgcgcgcc agcacggcat gcgtctgctc ggccccaact gtttgggcgt
      121 gttcgacgca cgcaccgcgt attacgcgac cttctcgtcg tccttcgatt ccggctggcc
      181 ggtgatgggc cgcatcggca tcgcgtcgca atcgggcgcg tatggcacgc atctctacac
      241 gctggcgcgc aaccgcggca tcggcgcgtc gctgtgcatc atgaccggga atgaggccga
      301 cgtgaccgtc ggcgaatgca tcggctggct ggcggagaat ccggaagtcg atgtgattgc
      361 cgcctatatc gaaggcatcc gtgaatctgc cagcctgatc gccgcgttcg aagcggcgcg
      421 tgcggcgcgc aagccgatca tcatgcagaa ggtcggccgc agcgaactcg gcagcaaggc
      481 ggcgaagtcg cacaccgcgt cgatcgccgg cgacgatgcg gtgaccgagg cggtgatgcg
      541 cgagttcggc gtggcgcgcg cgcgcaactc ggaagagatg ctggacatcg cccacaccgc
      601 aacgcggaag atctatccgg cacgcaactc gctgggggtg atcaccgtgt ccggcggcgc
      661 cggcgtgctg atcagcgaca ccgccgacag cctgggcctg gcgatgccgg aaatgccggc
      721 ggacacccag aaattcctgc gcgagctggt gccgttctgc gccccgcgca atccggtgga
      781 cgccaccgcg caggtgtcga acgacgtgaa gctgatcaac accttcaccg aggcgatggt
      841 gcgcgacggc ggctatgcct ccatcctcgg ctttttcagc atgaccgcat catcccgccg
      901 ctggccggcg atccgcgagc agctgaacct ggtgaaggcg cagtatcccg accggctata
      961 cgcgctgtcg gtgatcgtgc cgccggaacg gcgcgacgag ctggaagcgg atggctgggt
     1021 ggtgcacgag gacccgacgc gcgcggtggt ggcgatcgac gcgatgggcc gcttcggcgc
     1081 cgccttcgcc gcgccggccg gcgaaaagcc gcccgcggtg cacgccatta ccctgccgga
     1141 cagcacgccg tcggaagccg aggcgaaacg gctgctggcc tccgccggca tcacctccgc
     1201 cccggaacag gcctgcggca gcgcggatga agccgccgcc gcggcggagc tgttcggctt
     1261 cccggtggtg atgaagatcc tgtcgcccga catcctgcat aagagcgaga tcggcggcgt
     1321 gctgctgggc gtggcggacg ccgccgcggt gcgcgacggc tacgacctgc tgctggcgcg
     1381 ggcgaagcgc gcggcaccgc acgcgcgcat cgagggcgtg ctggtggcga agcagttgca
     1441 gggcggtgtg gaatgcatcc tgggcatcca ccgcgacccg gtgttcggcc cgatcgccat
     1501 gttcggcctg ggcggcattt tcgtggaggt gatgaaggac gtggtgttcc gccgctgccc
     1561 ctttggcgca gacgtggcgg agcagatgat ccgctcgatc aagggcgcgc cgctgctgct
     1621 gggcgcgcgc ggccgcccgc cggtggacgt gcacgcgctg gcgcagatgc tttcccgact
     1681 ttcagcattc gcagttgcgg cggggccgcg gctggtgtcg gtggatctga acccggtatt
     1741 cgccatgccg gcgggacacg gggcgtttgc ggtggatgcg gtggtggagg tggcgtagcg
     1801 cgacctgaca cgccggcggg ggtaccgggt cctggcggac gacggcgtta ggagcgttaa
     1861 gtgtaagcga tgatgctgcc aaccacgtcg tggcgggcga aggcccgcca cccacgactg
     1921 gctggggggg acttggttat cttctgcacg aagcatgcag taggtgacat tgggagaggc
     1981 agggtaagct ttttgccatc gtcgcgaggc aagagttcgc acgcggctgg cgatttcata
     2041 agaatgagac gcagaggaac gcagagcaga tctgatgatc ctagcagcct gtcggactta
     2101 acacgacggc ggcaaccgta ctagaatcgc aacgtcgatt cgcagcccgt ccggaccgcc
     2161 tgcatgagca attttcgccc gaccgaccgc aacacggact tcctgatgcc gccctcggtg
     2221 gatgagtggt tgccggagcg tcgcttggcg cggttcatcg ttgagatggt ggagcaactg
     2281 gacctgcggt cgatgcggat tcggtctggc ttctggtgtc cgacgccgcc gccgagatgg
     2341 ccgagccggg cttccaaccc agtccggccc aggtcgccag cgtgatcctg tccgtcgcgg
     2401 tgcgccgcct cgggccggac ctcaagcgcg atgccacgca agcactcaag gcggccagcg
     2461 ggtcgacata gcggttgacc ggcaacgcct caggcagcgt aacccagtgc ccgcggaagt
     2521 cgccggcttg atcgcattcc gcttgctcga tcggtttgcc gtgtatcgga atagccaggg
     2581 tagaccagtc ggcccttcag cccggtcacc acgactcgca ggcaaccccg tcgctgtcac
     2641 cgtccagcca gggcgcataa tagggtgagc ccctcggaat gttcaccagc cccatggcgc
     2701 gcgcctggg
//
LOCUS       DASSZH010000575         2165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1189863, whole genome shotgun sequence.
ACCESSION   DASSZH010000575 DASSZH010000000
VERSION     DASSZH010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189863"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VFN42_12475"
     CDS             <1..767
                     /locus_tag="VFN42_12475"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8997474.1"
                     /translation="GAVKLADMEEYIRVVQALWRGETVDFTMEGKTRPIRLLNPDLGL
                     INIHDPIPLFIAASGPRARALTAKLGAGWIDNLADAQRGAPTLQAMRATWTKAGHASD
                     TLTAVAWLSGAVLEEGEAADGPRAMALAGPRAATLLHRAADAALADHPAPLSMRPEFA
                     AQVEAYVAHARGFRPQGAYYLQNHRGHLMFVRPDERPFITADMLRNTSFIGTVRELMP
                     RFEMLRAAGFHQAVLSIPPGQEHAIEDWSRILRAMG"
     gene            complement(775..1452)
                     /locus_tag="VFN42_12480"
     CDS             complement(775..1452)
                     /locus_tag="VFN42_12480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HET8997475.1"
                     /translation="MPRVQAAIFDAYGTLLDVHAAMQAHAPRIGTNWQAISADWRQKQ
                     IEYTWVRSLAGKAHHRDFWHLTEEALAWAAAKHGIGDAALLAEVLQAYRQLSVYPEAK
                     PMLQRLRDMGIARAILSNGEPDMLADGVQSAGIDGLLDDVLSAESAGVFKPDARVYRL
                     ATDRFGLQAGQMAFVSSNPWDAFGASAFGFQVFWVNRHKQPAEYDLHQRATELPDLSA
                     LAEKLAG"
     gene            complement(1455..>2165)
                     /locus_tag="VFN42_12485"
     CDS             complement(1455..>2165)
                     /locus_tag="VFN42_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET8997476.1"
                     /translation="HSAGVLRSVEAIKRQSNTVQVIAGNVATPEGAIALIEAGVDAVK
                     IGIGPGSICTTRVVAGVGVPQFTAVLECAAACRERGVPAIADGGMRTSGDIVKALAAG
                     ADAVMIGSLLAGTDEAPGEVFLYQGRSYKSYRGMGSIGAMARGSADRYFQQDIKDQLK
                     LVPEGVEGMVPYKGPVDQAIHQLVGGVRAGMGYTGSGTITDLQHNAKFRRITGAGLRE
                     SHVHDVSVTREAPNYRES"
BASE COUNT          316 a          763 c          744 g          342 t
ORIGIN      
        1 acggcgcggt gaagctggcc gacatggaag agtacatccg ggtcgtgcag gcgctgtggc
       61 gcggcgagac ggtggatttc accatggaag gcaagacacg gccgatccgg ctgctgaacc
      121 ccgatctcgg gctgatcaac atccatgacc cgatcccgct gttcatcgct gcctccggcc
      181 cgcgggcgcg ggcgctgacc gccaagctgg gcgccggctg gatcgacaac ctggcagatg
      241 cgcagcgcgg cgcacccacg ctgcaggcga tgcgtgccac ctggacgaag gcgggccatg
      301 catcggacac gctgaccgcg gtggcctggc tcagcggcgc cgtgctggag gagggggagg
      361 ccgcggatgg tccgcgcgcc atggccctgg cgggcccgcg cgccgcgacg ctgctgcacc
      421 gtgccgcgga cgccgcattg gcggaccatc ccgcgccact gtcgatgcgt ccggaattcg
      481 ccgcgcaggt ggaggcctat gtcgcgcacg cgcgcggttt ccggccgcag ggtgcgtact
      541 atctgcagaa ccaccgcggc cacctgatgt tcgtgcgtcc cgacgagcgg cccttcatca
      601 ccgcggacat gctgcgcaac accagcttca tcggcacggt gcgggagctg atgccgcgct
      661 tcgagatgct gcgcgccgcc ggcttccacc aggcggtgct gtccattccg cccgggcagg
      721 agcacgcgat cgaggactgg agccggattc tccgcgcgat gggctgagca ggcgctaccc
      781 ggccagtttc tccgccagcg cggacagatc gggcagctcg gtcgccctct ggtgcaggtc
      841 gtactcggcc ggctgcttgt ggcggttcac ccagaacacc tggaagccga acgcgctggc
      901 gccgaaggca tcccaggggt tggacgagac gaacgccatc tgcccggctt gcaggccgaa
      961 ccggtcggtg gccagccggt agacccgggc atccggcttg aacacgccag cggattccgc
     1021 gctgagcacg tcgtccagca ggccgtcaat tccggctgat tgcacgccgt cggccagcat
     1081 gtccggttcg ccgttggaca ggatggcgcg ggcaatgccc atgtcgcgca gccgctgcag
     1141 catcggcttc gcctccggat acaccgagag ctgccgatac gcctgcagca cctcggccag
     1201 cagcgccgca tcgccgatgc cgtgcttcgc cgctgcccag gccagcgctt cctcggtcag
     1261 gtgccagaag tcgcgatgat gcgccttgcc ggccaggctg cggacccagg tgtattcgat
     1321 ctgcttctgc cgccaatcgg cgctgatcgc ctgccagttc gtgccgatgc gcggcgcgtg
     1381 cgcctgcatc gccgcatgca catccagcaa cgtgccatag gcgtcgaaga tcgcggcctg
     1441 gacgcggggc atggtcagct ctcccggtag ttgggcgcct cacgggtgac cgagacgtcg
     1501 tgcacgtggc tctcccgcag gccggcgccg gtgatgcggc ggaacttcgc gttgtgctgc
     1561 aggtcggtga tggtgccgct gccggtgtag cccatgccgg cacggacgcc gccgaccagt
     1621 tggtggatcg cctgatcgac cggccccttg taggggacca tgccttccac cccctccggc
     1681 accagcttga gctggtcctt gatgtcctgc tggaagtagc ggtcggcgga accgcgcgcc
     1741 atggcgccga tgctgcccat gccgcgatag gatttgtacg agcggccctg gtagaggaac
     1801 acctcgcccg gcgcctcgtc ggtgccggcg agcaggctgc cgatcatcac cgcatcggcg
     1861 ccggcggcca gcgccttcac gatgtcgccg ctggtgcgca ttccgccatc ggcgatcgcc
     1921 ggcacgccgc gttcgcggca ggcggcggca cattccagca ccgcggtgaa ctgcggcacg
     1981 ccgacgccgg ccaccacgcg tgtggtgcag atgctgcccg ggccgatgcc gatcttcacc
     2041 gcgtccaccc cggcttcgat cagcgcgatc gcgccctcgg gcgtcgccac attgccggcg
     2101 atcacctgga cggtattgga ctggcgcttg atggcctcca ccgaacgtag cacgcccgcc
     2161 gaatg
//
LOCUS       DASSZH010000576         3162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_235172, whole genome shotgun sequence.
ACCESSION   DASSZH010000576 DASSZH010000000
VERSION     DASSZH010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235172"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VFN42_12490"
     CDS             complement(<1..839)
                     /locus_tag="VFN42_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8997477.1"
                     /translation="MDQRLIELASRIAEALDRLAPPPQPPVALDGADAFVWHPAPPRL
                     APVRRVSRVEIGLLQGVERQKQILLENTERFAEGLPANNAMLWGARGMGKSSLVKAVH
                     AVINARDPGRLVLIEIHREDIRTLPDLLNVLREQPRRCLILCDDLSFEKEDSDYKALK
                     SILEGGIEGRPDNVLFYATSNRRHLMSRDMIENERSTAINPNEAIEEKVSLSDRFGLW
                     LGFHNCDQDTFFAMIDGYAAALRLPIEAEALHAQAVEWSVTRGSRSGRVAWQFIQDLA
                     GKLG"
     gene            973..1302
                     /gene="yajC"
                     /locus_tag="VFN42_12495"
     CDS             973..1302
                     /gene="yajC"
                     /locus_tag="VFN42_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003624536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET8997478.1"
                     /translation="MFGSAAQFLPLILIFGVFYFLLIRPQQQKQKEAKAMLSAIKRGD
                     RVVTGGGILGVVQRVPMVQDKDGKQVPSNEIEVEIAPNLRVTVLRETIASVIRPTAAN
                     DSKPAKA"
     gene            complement(1350..1979)
                     /locus_tag="VFN42_12500"
     CDS             complement(1350..1979)
                     /locus_tag="VFN42_12500"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HET8997479.1"
                     /translation="MRRIIWGRATSSNVMKVLWGLGELNLAFERIDVGGSFGKTDTAE
                     YRAMNPTGLVPTLQEDDFTLWESNVILRYLCHAHAAHSLLWPQDPRERANIDRWMDAQ
                     QTQLNRPMAAVFWGLVRTPPDKRDPAAIRQGVADAAKAWNLIGAGLAKRPYVAGDSFT
                     LADIPWGVHAHRWFNIEFDRPEVPGLRAWYDRLCERQAYQAAVVSCPIV"
     gene            2073..2573
                     /locus_tag="VFN42_12505"
     CDS             2073..2573
                     /locus_tag="VFN42_12505"
                     /inference="COORDINATES: protein motif:HMM:NF014639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HET8997480.1"
                     /translation="MPRVSLRTVALLSLLVAFAALAFARSSEYWGGFVPCALCLLERW
                     PLRIVIVLGMLGMILPRPASRVLLGLAALTMLGDAAIAFVHIGVEFHWWRSPLPECAA
                     PVFHGGTIAERLASMPARPSKPCDQPTFLIPGLPVSFAMMNLWFSLAFAAVLGLFVWN
                     AEERRP"
     gene            2570..3115
                     /locus_tag="VFN42_12510"
     CDS             2570..3115
                     /locus_tag="VFN42_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HET8997481.1"
                     /translation="MTDTARWPGDSTPRERVERIIRVDHAGEYGAARIYAGQLAVLHR
                     SDKAALLRHMQDQEQQHLERFSDLVVRRRVRPTAMLPFWHLAGFALGALTAALGERAA
                     MACTVAVEETIDAHYSGQIAELNESEAELRGTIETFRAEELEHRDIALTHGATQAPGY
                     RLLSAAIKAGCRAAIAISERI"
BASE COUNT          491 a         1091 c         1058 g          522 t
ORIGIN      
        1 cccagcttcc ccgccaggtc ctggatgaac tgccacgcca cccgccctga ccgtgagccg
       61 cgggtgaccg accactccac cgcctgcgca tgcagtgcct cggcctcgat cggcagccgc
      121 agcgccgcgg catagccgtc gatcatggcg aagaaggtat cctggtcgca gttgtggaag
      181 cccagccaca ggccgaaccg gtcggacagc gacaccttct cctcgatcgc ctcgttcggg
      241 ttgatcgcgg tggaccgctc gttctcgatc atgtcgcgcg acatcaggtg gcggcggttg
      301 gaggtggcgt agaacagcac gttgtccggc cgcccctcga tgccgccctc caggatcgac
      361 ttcagcgcct tgtagtcgga gtcctccttc tcgaaggaca ggtcgtcgca caggatcagg
      421 cagcggcgcg gctgctcgcg cagcacgttc agcaggtccg gcagggtgcg gatgtcttcg
      481 cggtggatct cgatcagcac cagccggccg gggtcgcgcg cattgatcac cgcgtgcacc
      541 gccttgacca gcgaggactt gcccatgccg cgggcgcccc acagcatggc gttgttggcc
      601 ggcaagccct cagcgaagcg ctcggtgttc tccagcagga tctgcttctg ccgctccacc
      661 ccctgcagca ggccgatctc cacccgcgac acgcggcgga ccggggccag gcgcggcggc
      721 gcggggtgcc agacgaaggc gtccgccccg tccaaggcca ccggcggctg cggcggcggg
      781 gccagccggt ccagcgcctc ggcgatcctg ctggcgagtt caatcagacg ctggtccatg
      841 cggcggttcc gaacaacttg acgggaaggg ggcgcaaact atggtcccgg ccaattcccc
      901 gcaaggacct ggaccccgat gcactttttt gagctgatct cgcctgccta cgccgccggc
      961 agtcccgacg cgatgttcgg cagcgccgcg cagttcctgc cgttgatcct gatcttcggg
     1021 gtgttctact tcctgctgat ccggccgcag cagcagaagc agaaggaggc gaaggccatg
     1081 ctgtccgcga tcaagcgcgg cgaccgcgtg gtgaccggcg gcggtattct gggcgtggta
     1141 cagcgcgtgc cgatggtgca ggacaaggac ggcaagcagg tgccctcgaa cgaaatcgag
     1201 gtggagatcg cccccaacct gcgggtgacg gtgctgcggg agacgatcgc cagcgtgatc
     1261 cggccgaccg ccgcgaacga cagcaagccg gcgaaggcat agcaagccga ggctccctct
     1321 ccgccgggaa ggcggagagg atacccgagc tatacgatcg ggcagctcac cacggcggcc
     1381 tggtaggcct ggcgctcgca cagccggtcg taccaggccc gcaggcccgg gacctccggg
     1441 cggtcgaatt cgatgttgaa ccagcggtgc gcgtgcacgc cccagggaat gtccgccagg
     1501 gtgaagctgt cgcccgcgac atagggacgc ttcgccagtc cggcgccaat caggttccag
     1561 gccttggctg catcggccac gccctggcgg atcgccgccg ggtcgcgctt gtcgggcggc
     1621 gtgcggacca ggccccagaa caccgccgcc atcgggcggt taagctgggt ctgctgcgca
     1681 tccatccaac ggtcgatatt ggcgcgctcc cgcgggtcct gcggccatag cagggaatgc
     1741 gccgcgtgcg cgtggcagag gtagcgcagg atcacgttgc tttcccacaa cgtgaaatcg
     1801 tcctcctgca acgtgggcac caggccggtc gggttcatcg cccggtattc cgccgtgtcg
     1861 gtcttgccga acgagccgcc gacgtcgatc cgctcgaagg ccaggttcaa ctcacccaac
     1921 ccccacaata ccttcatgac gttgctggac gtggcgcgtc cccagatgat tcggcgcatg
     1981 cgaccctccg ctttccgacc ggcgatgagc ctaggcagcc gttaacgact cggcaacttg
     2041 cctcgggcat aaatccgtcg cacatgccgc atatgccacg cgtttccctc cgcaccgttg
     2101 ccctgctcag cctgctggtc gcttttgccg cgctggcgtt cgcccgcagc tcggaatact
     2161 ggggcggttt cgtgccctgc gccctgtgcc tgctggagcg ctggccgctg cgcatcgtca
     2221 tcgtgctcgg catgctgggg atgattctgc cgcgccccgc cagccgggtg ctgctgggac
     2281 tcgcggcgct caccatgctc ggcgacgcgg cgatcgcctt cgtgcatatc ggggtcgaat
     2341 tccactggtg gcgcagcccg ctgccggagt gtgccgcgcc ggtcttccat ggtggcacca
     2401 tcgccgaacg gctggcctcg atgccggctc ggccatccaa gccctgcgac cagcccacct
     2461 tcctgatccc cgggctgccg gtttcctttg cgatgatgaa cctgtggttc tcgctggcct
     2521 tcgccgccgt gctagggttg ttcgtctgga atgctgagga gcggcggcca tgaccgacac
     2581 ggcgcggtgg ccgggcgatt cgacgccacg cgagcgggta gagcgcatta tccgggtgga
     2641 ccacgccggt gaatacggcg cggcccgcat ctatgccggc cagctggcgg tgctgcaccg
     2701 cagcgacaaa gccgcgctgc tgcggcacat gcaggaccag gaacagcagc acctggagcg
     2761 cttctccgac ctagtggtaa ggcgtcgggt acggccaacc gcgatgctgc cgttctggca
     2821 ccttgccggc ttcgcgctgg gcgcgctgac ggcggcgctg ggcgaacgcg cggcaatggc
     2881 ctgcaccgtc gcggtggagg agacgatcga cgcccactac tccggccaga ttgccgagct
     2941 gaacgaatcg gaagcggagc tgcgcggcac gatcgagacg ttccgggcgg aggagttgga
     3001 gcaccgcgac atagctctga cccatggcgc gacgcaggcg cccggctacc ggctgttgtc
     3061 ggccgcgatc aaagccggct gccgcgcggc gatcgcaatc agcgagcgga tctgacttcg
     3121 gcgccggcca ctcattccgc ctcgatcacc gcgcggagca tc
//
LOCUS       DASSZH010000577         2084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_780926, whole genome shotgun sequence.
ACCESSION   DASSZH010000577 DASSZH010000000
VERSION     DASSZH010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780926"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..1112)
                     /locus_tag="VFN42_12515"
     CDS             complement(189..1112)
                     /locus_tag="VFN42_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HET8997482.1"
                     /translation="MPIPIILDCDPGTDDAIALLLALASPELEVRAITVAGGNVGLAD
                     TLRNALALTRLAGSPAPVFAGADRPLLGAFTNEPHVHGADGLGGIALPDGGAPAREIA
                     ADAIRRILREATEPVTLVGIAPVTNLALALMTESSLAAKVAQIVLMSGAWGEGNATPA
                     AEFNALNDPEALAVLLACGRPVVFATLELTAQALATPARIAAWRALGGGRCLTTACDI
                     QARVPPSRRLGGMGAALHDPCALAWLIRPELFTSRPCAVWMDLGPGPSRGRTVIDRWR
                     RTDAPHNAVVLETLDADGFFALLGERLTGLP"
     gene            1177..1737
                     /locus_tag="VFN42_12520"
     CDS             1177..1737
                     /locus_tag="VFN42_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997483.1"
                     /translation="MSQETHLAVHVPAYAGTLLPQPRDRLRQLRKHLLESLRAERQMR
                     DRAAAVGPAAPEPPAPVGEIAPTACALCQGWCCSKGGTHAYLDERTMARVRHARPELD
                     AWGILRLYTRAAADPAYAGSCVFHGAQGCTLDRSLRSDLCNAYFCSGLASLVKQADVP
                     GRVVLTAAKGTIIRTSVMTLGGAAHQ"
     gene            complement(1702..>2084)
                     /locus_tag="VFN42_12525"
     CDS             complement(1702..>2084)
                     /locus_tag="VFN42_12525"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8997484.1"
                     /translation="SYYWRLEDSIASARAFNNRFRALNDGRVPSDYGALGFAGVMTVL
                     TAAKHAGSVATEKLVDALRGMKYDLYKGAETYRTCDNQAVQSVLIVESRFTADPNDLD
                     VFRIAHLDPASESLLMGCAAEGHH"
BASE COUNT          332 a          746 c          703 g          303 t
ORIGIN      
        1 tacggcgtgg tgtagccgat cgccgtgtaa taggcgcggg tgcgcgccat gtagggaatc
       61 ggcgcatcga aatccggcgc gaagccgagt cgggtatcga gatcgtcctg gtccatggcg
      121 aagcctccgt ggcgcaatgc cagactcagg cttgacggga acccggtgtc aaccgccggc
      181 gcgctcggct acggcagccc agtcagccgc tcgcccaaca gcgcgaagaa tccatccgcg
      241 tcgagcgtct ccagcaccac cgcattgtgc ggcgcatcgg tccgtcgcca gcggtcgatc
      301 accgtgcgtc cgcggctggg tcccggcccc agatccatcc acaccgcgca gggccgggaa
      361 gtgaacagct ccggccggat cagccaggcc agcgcacagg gatcgtgcag cgccgcgccc
      421 atgccgccca gccgccgcga cggcggcacg cgagcctgga tgtcgcacgc ggtcgtcagg
      481 cagcgcccgc cacccagcgc ccgccacgcg gcgatccgcg ccggggtcgc cagcgcctgt
      541 gcggtcaact ccagggtcgc gaacaccacg ggccggccac aggccagcag cacggccagc
      601 gcctcggggt cgttcagcgc gttgaattcc gcggccggcg tggcgttgcc ctcgccccag
      661 gcaccgctca tcagcacgat ctgcgccacc ttcgccgcaa gtgaggactc ggtcatcaag
      721 gccagcgcca ggttggtgac cggtgcgatt cccaccagcg tgaccggttc cgtcgcctcg
      781 cgcaggatcc ggcggatcgc gtcggccgcg atctctcgag ccggcgcgcc gccgtccggc
      841 agcgcgatgc cgcccagccc atccgccccg tgcacatgcg gctcgttggt gaaggcgccc
      901 agcagcgggc gatcggcgcc ggcaaacact ggggcagggg agccggccag gcgcgtcaat
      961 gccagcgcgt tgcgcagcgt gtccgccagc ccgacattgc cgccggcaac ggtgatcgcg
     1021 cgaacctcca gttcgggtga ggcaagggcg agcaacagcg caatcgcatc gtcggtgccg
     1081 ggatcgcaat ccaggataat cgggatcggc acgccgccct ccttgaaatc agcggcggca
     1141 cggtgccacg gtccgccgcc aaggagccag acccgcatgt cccaggagac tcatctggcg
     1201 gtgcacgtgc ccgcctatgc tggcacgctg ttgccgcagc cgcgcgaccg actgcggcag
     1261 cttcgcaagc acctgctgga gtcgctgcgc gcggaacgcc agatgcggga ccgcgcggcc
     1321 gcagtcggtc cggccgctcc ggagcctccg gcccccgttg gcgagatcgc gcccaccgcc
     1381 tgtgcgcttt gccagggctg gtgctgcagc aaaggcggca cacatgccta tctggacgaa
     1441 cgcacgatgg cgcgcgtgcg tcatgcccgg ccggagctgg atgcctgggg catcctgcgg
     1501 ctttacaccc gcgcagccgc ggaccccgcc tatgcggggt cctgcgtgtt ccacggcgcg
     1561 caaggctgca cgctggaccg cagcctgcgc tccgacctgt gcaacgccta tttctgcagc
     1621 ggactggcgt cgctggtgaa gcaagccgat gtgccggggc gtgtcgtgct gaccgcggcg
     1681 aagggcacca tcatacggac gtcagtgatg accctcggcg gcgcagccca tcaataggga
     1741 ctcgcttgcc gggtcgagat gcgcgatgcg gaaaacgtca agatcgttgg ggtcggcagt
     1801 gaaccggctc tccacgatca gcaccgattg cacggcctgg ttgtcgcagg tgcggtatgt
     1861 ttccgcgccc ttgtacaggt cgtatttcat cccgcgcagg gcatccacca gcttctcggt
     1921 ggcgacgctg cccgcgtgct tcgccgccgt tagcaccgtc atcacgccgg caaacccaag
     1981 cgcaccgtaa tccgacggca cgcgtccgtc attcagcgcg cggaagcggt tgttgaacgc
     2041 gcgcgcagac gcgatgctgt cctccaggcg ccaatagtag gacg
//
LOCUS       DASSZH010000578         4768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1582327, whole genome shotgun sequence.
ACCESSION   DASSZH010000578 DASSZH010000000
VERSION     DASSZH010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582327"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1342
                     /locus_tag="VFN42_12530"
     CDS             <1..1342
                     /locus_tag="VFN42_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997485.1"
                     /translation="PRMRVAPAVRPLPAISGPLVQALPAIAGPAAPYTASERLGARRH
                     RTYTLAAILGAATSLAALLLSSLGPSDPFALFQHPARHAAVAVSPAAAKPRHPVQQAA
                     VLPPPADAISHAATIQRISEPVVPRQAVPPPEHSEAVAEPMQQPKPAATRVMPAAAAA
                     RPAAVTHAAASAAKPLPVQVARSASTLAEPTPVQDAKPVKASRITVSPAPPPVVGVAQ
                     AASLPAHGMLRAEHASKPVPAVDTAAPPAPPPMVRVASVPSPAQVADPEPAAAPDPKS
                     IHVAEPAPPQTARASGVRQVDVVQASDARPASIVPATPAKSAAVPAAGDTPTAPQEIA
                     GEQHVAGAVSKPVHASRVAEADRPGAKPVRAAEKPAVAHPVRHELAETGAATHRKQIV
                     RSERQVAARRPLSGHAEVERLNEQSLAAARKGQEYHPAASVQAAAPVHRAAQAM"
     gene            complement(1421..2140)
                     /locus_tag="VFN42_12535"
     CDS             complement(1421..2140)
                     /locus_tag="VFN42_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET8997486.1"
                     /translation="MSADLDRRRLLGRALAFGAAGLLTGCNYDDLQHPDFADRALRAV
                     SRWNDKVQAALFNPNTLAPTYPASAITRPPRFNAFYDESHVHVVDGASWNLELSGLIQ
                     DKRPWRLADLRAAPQESQITRLICVEGWRVVGQWGGVPLAWFLRRIGADTRARYVSLH
                     CADGYYESIDMQSALHPQTLLALDFLGQPLTPAFGFPLRLRVPTKLGFKNPKSLVGLG
                     VTNVFPGGYWVAQGYNWFSGS"
     gene            complement(2137..2754)
                     /locus_tag="VFN42_12540"
     CDS             complement(2137..2754)
                     /locus_tag="VFN42_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HET8997487.1"
                     /translation="MPGRKSRPIHPWPLRLMHWLNATAIVVMIGSGWQIYNASPLFAF
                     SFPARITIGEWLGGAIAWHFAAMWLFALNGIAYLTWGTLSGHFRHKLRPPSPRAFWRD
                     TLLALRFRLDHQHGVYNAVQRALYLGVIALGVLALASGLAVWKPVQLWWLSDLFGGFA
                     LSRYVHFFAMAGIVGFLAVHLLLVALVPKVLWPMINGGRLSEDLR"
     gene            2922..3164
                     /locus_tag="VFN42_12545"
     CDS             2922..3164
                     /locus_tag="VFN42_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997488.1"
                     /translation="MTRFILACALAGGLALAGGVSAQAASDTGTQQQSTMGNSSMGHD
                     TMGHDTMGHDSMGHSSMGHDSMGHNAMGNGASTDKK"
     gene            3265..3915
                     /locus_tag="VFN42_12550"
     CDS             3265..3915
                     /locus_tag="VFN42_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HET8997489.1"
                     /translation="MPRLRDQLEAKRETMNDDVRRAYEAMVAELQRTDFLTHALQVGD
                     RFPDLVLPNAEGALVSISALLEHGPLVVTFFRGEWCPYCRLMLDALAATLPEIMALGA
                     SLVAVTPEAGGRALTAKQNHKAGFEVLCDVDCGVGLSCGVVFRAPEPYRLLLLKYGTD
                     LAERHGNPSWFLPVPATFVVDRQGIVRWRFLSVDFTERAEPADLLAALRSLPAPEG"
     gene            3920..>4768
                     /locus_tag="VFN42_12555"
     CDS             3920..>4768
                     /locus_tag="VFN42_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET8997490.1"
                     /translation="MELHQVRYFVTLGRTLNFTRAAAECNVTQPALNRAIQKLEDELG
                     GPLLFRERTLTRLTPLGRSMMPHLTAMLTAAETARRIADEELGRQQMSLRVGLGPAIA
                     AARVADVVGQLTRFCPSLAVQFEEAGSGTLVEYMLSDMLDCALLPDSPELPDRLHRWK
                     LCDEGCVVVLPAGHPLALRDSLGVDELSGETLLLGERCGGFALRLIEASQESFTVRRC
                     GGSWVQMLDLVAAGLGLALLPDSLAIGAGLVSRPLARPVLQRSVLLTLVAGRPQGTAV
                     ANFVKLC"
BASE COUNT          749 a         1652 c         1657 g          710 t
ORIGIN      
        1 accccgcatg cgtgtcgcac cggcggtgcg cccgcttccg gcgatcagtg gtccgctggt
       61 gcaagcgttg cctgccattg ccggtccggc cgcgccatac accgcttcgg agcggctggg
      121 cgcgcggcgc cacaggacct acacgctggc cgccatcctt ggggccgcga ccagcctggc
      181 ggcgctgctg ctgagcagcc tgggtccgtc ggacccgttc gccctgttcc aacacccggc
      241 gcggcacgcg gctgtcgcgg tgtcacccgc cgctgccaag ccgcggcatc ccgttcagca
      301 agcggctgtg ttgccacccc cggccgacgc catatcccat gccgccacca tccagcgtat
      361 ctcggagccg gtcgtgccga gacaggcggt gccgccgccc gagcactccg aagcagtcgc
      421 ggagccgatg cagcaaccga aaccggcggc aacacgcgtc atgcctgccg cggcagccgc
      481 gaggccagcg gcggtgaccc acgccgctgc gtccgcggcc aagccgctgc cggtgcaggt
      541 ggcgcgttcc gccagcacgc tcgccgagcc gacacccgta caggacgcca agccggtgaa
      601 ggcgtcccgg atcaccgtat cgccggctcc gccgcccgtg gtgggggtgg cgcaagccgc
      661 cagcctgccg gcccacggca tgctgcgtgc cgagcacgcc agtaagccgg tgccggcggt
      721 cgataccgct gcaccaccgg ccccgccgcc catggtgcgg gtcgccagcg ttccatctcc
      781 ggcgcaggtt gccgacccgg agccggcggc cgctcctgat ccaaagtcca ttcacgtcgc
      841 cgagcccgcc ccgccacaga ccgcccgcgc ctccggcgtc cggcaggtgg atgtggttca
      901 ggcaagcgat gccaggccgg cttcaattgt gcccgccaca ccggccaagt ctgccgctgt
      961 gccggccgcc ggcgacacgc cgacggcgcc gcaggaaatc gctggcgaac agcatgtggc
     1021 cggggcggtc agcaagcccg tgcacgcatc ccgagtggcc gaagcagacc gacccggcgc
     1081 caagccggtt cgggctgccg aaaaacccgc cgtggcgcat cccgtccggc acgagcttgc
     1141 cgaaacggga gccgccacgc atcgcaagca gatcgttcgt tcggaacggc aggtcgcggc
     1201 ccggcgcccg ctctccggcc atgccgaggt cgagcggctg aatgagcaga gcctggccgc
     1261 cgcgcgcaaa ggccaggagt accatccggc ggcttccgtg caggcagcgg cgccggtgca
     1321 ccgggcggcg caggcgatgt gagggcccgc ggcgaggctg cctgacgaag caaaggccgg
     1381 cctcgacggg gcatcgcgtc cgcgtcaacg cgcgcgggca tcaggatccg ctgaaccagt
     1441 tgtagccctg tgcgacccag tatccgcccg ggaacacgtt ggtgactccg aggccaacca
     1501 gcgacttcgg attcttgaat cccagcttgg tcggaacccg cagccgcagc ggaaagccga
     1561 aagccggggt gagcggctgg cccaggaaat ccaatgccag aagcgtctgt gggtgcagcg
     1621 ccgattgcat gtcgatgctt tcgtaatagc cgtcggcgca atgcagcgac acgtagcgtg
     1681 cccttgtgtc ggcgccgatc cggcgcagga accaggccag cggcaccccg ccccattggc
     1741 ccaccacgcg ccagccctcg acgcagatca gccgggtgat ctggctttcc tgcggcgcgg
     1801 cgcgcagatc ggccagccgc cacgggcgct tgtcctggat caacccgctc agttccaggt
     1861 tccagctggc gccatcgacc acatgcacgt ggctctcgtc gtagaacgcg ttgaaccgcg
     1921 gcggccgggt gatggcactg gccggatagg ttggcgccag cgtgttcgga ttgaacaatg
     1981 ccgcctgcac cttgtcgttc cagcgcgaga cggcgcgcaa ggcgcggtcg gcgaagtccg
     2041 ggtgctgcag gtcgtcgtaa ttgcagccag tcagcaagcc cgccgcacca aaggcgagcg
     2101 ctcggccgag caggcggcgg cggtccagat cggcgctcat cgcaggtcct ccgacaggcg
     2161 cccgccgttg atcatcggcc acagcacttt cggcaccagc gccaccagca ggagatgcac
     2221 ggccaggaaa ccgacgatcc cggccatggc gaagaagtgc acgtagcggg acagggcaaa
     2281 gccaccgaac agatcggaca gccaccaaag ctgcaccggc ttccagacgg ccagcccgga
     2341 cgcgagcgcg agcacgccca gagcgatgac gcccaggtac aacgcacgct gcaccgcgtt
     2401 gtacacgcca tgctggtggt ccagccggaa gcgaagcgcc agcagcgtat cgcgccagaa
     2461 cgcgcggggg ctgggcggtc gcagcttgtg gcggaaatgc ccgctcagcg tgccccaggt
     2521 caggtaggcg atgccgttca gcgcgaacag ccacatggcc gcgaagtgcc aggcgatggc
     2581 gcccccgagc cattcgccga tcgtgatgcg ggcaggaaag ctgaaggcga acagcgggga
     2641 cgcgttgtag atctgccagc cgctgccgat catcacgacg atcgcggtgg cgttcagcca
     2701 gtgcatcagc cgcagcggcc atgggtgaat cgggcgggat ttcctgccgg gcatatggat
     2761 cctcctccgg atggcgattg cccggcttct ccaccgccgc aagcggcccg gtccatgcat
     2821 ggagacgata gccacacggc attggccaca cgcggcggcg gtcgcgatcc tggctgggcc
     2881 gggcagtcgt ctcgcccgct ttcatcccac ggaggaacac gatgacacgc ttcattctcg
     2941 catgcgccct ggcgggcggg ctggccctgg cgggcggcgt ctcggcgcag gcagcatccg
     3001 acaccggcac gcagcagcag agcaccatgg gcaattcttc catgggccac gacacgatgg
     3061 gtcacgacac gatgggccac gattcgatgg gccattcgtc catgggtcat gattccatgg
     3121 gccataacgc gatgggcaac ggcgccagca ccgacaagaa gtgacgtccc gtcgctgcac
     3181 ggcgggacgg caccctgccg cccgccgtga cggcgccttg cccgccacct ggcaaccggg
     3241 cagggtcggt tctggagaag catgatgccg cgcctgcgcg accagctcga agccaaacgc
     3301 gaaaccatga acgacgacgt ccgccgcgcc tatgaggcga tggtggccga gttgcagcgt
     3361 accgacttcc tcacccatgc cttgcaggtg ggtgaccggt tccccgatct cgtcctgccg
     3421 aacgccgaag gcgcgctggt gtcgatcagc gccttgctgg aacacggtcc gttggtggtg
     3481 accttcttcc gcggcgagtg gtgcccctat tgccgcctga tgctggacgc gcttgccgcg
     3541 accctgccgg agatcatggc gctgggcgca tcgctggtcg cggtgacacc ggaagccggc
     3601 ggacgtgcgc tgactgccaa gcagaatcac aaggccggct tcgaggtgct gtgcgatgtg
     3661 gactgcggcg tcgggctgag ctgcggcgtg gtgttccgcg cgcccgaacc ctatcgcctg
     3721 ctgctgctga aatacggcac cgacctggcg gaacgccacg gcaatccgtc ctggttcctg
     3781 ccggtgccgg cgacctttgt ggtcgatcgg cagggcatcg tgcgctggcg cttcctcagc
     3841 gtcgatttca ccgaacgcgc cgaaccggcc gatctgctgg cggccctgcg ctccttgccg
     3901 gcgcccgagg gctagcgcga tggagctgca ccaggtccgc tacttcgtca cgctcggccg
     3961 cacgctgaat ttcacgcggg ccgcggcgga gtgcaacgtg acgcagccgg cgctgaaccg
     4021 cgccatccag aaactggaag acgagttggg tggcccgctg ctgtttcgcg agcggaccct
     4081 gacccggctg acgccgctgg gccggtcgat gatgccgcat ctgaccgcga tgctcaccgc
     4141 ggcggagacg gcgcggcgga tcgccgatga ggaacttgga cgccagcaga tgagtctgcg
     4201 ggtcggcctg gggccggcga tcgccgcggc gcgggtcgcc gacgtggttg ggcagctcac
     4261 ccggttctgc ccctcgctgg cggtgcagtt cgaggaggcc ggttccggca cgctggtcga
     4321 atacatgctg tcggacatgc tggattgcgc attgctgccg gattcgccgg agctgccgga
     4381 ccggctgcac cgctggaagt tgtgcgacga aggctgcgtg gtggtgctgc ctgccggtca
     4441 tccgctcgcg ctgcgggaca gcctgggggt cgacgagctg tccggggaga cgctgctgct
     4501 tggcgagcgc tgtggcggct tcgccttgcg gttgatcgaa gccagtcagg aatcgttcac
     4561 ggtccgccgc tgcggcggct cctgggtgca gatgctggat ctggtggcgg cggggctggg
     4621 actggcgctg ctgccggatt ccctggcgat cggtgccggc ctggtgtcgc gtccgctcgc
     4681 tcggccggtg ctgcaacggt cggtgctgct gacgctcgtg gctggccggc cgcaggggac
     4741 cgcggtggcg aatttcgtca aattgtgc
//
LOCUS       DASSZH010000579         2297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_848896, whole genome shotgun sequence.
ACCESSION   DASSZH010000579 DASSZH010000000
VERSION     DASSZH010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848896"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(270..956)
                     /locus_tag="VFN42_12560"
     CDS             complement(270..956)
                     /locus_tag="VFN42_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HET8997491.1"
                     /translation="MTWFYAFIPVIVASLGAAWTSWREPTPKLVGAVQHLAAGVVFYA
                     AAGEILPDAIHQGAVWTLVSGGVVGIAGMLLLRHLTEGAEESPVGLVAASAVDALIDG
                     LVLGLGFNAGQRQGTLLAIALAVEFLFLGLSIAGSFGKDAGRWTVIGTTIGVSLGVPL
                     GVLIAQPIGGLPHTWQAVAFAFGLIALLYLVTEELLTEAHERPETAWGAGAFFVGFLA
                     MAVISELMGK"
     gene            complement(976..1392)
                     /locus_tag="VFN42_12565"
     CDS             complement(976..1392)
                     /locus_tag="VFN42_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012092652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET8997492.1"
                     /translation="MTESLGIGAVAKATGIKVETVRWYERVGLLPQPERTAGNYRSYG
                     PAHLARLSFIRRARELGFSLDQVRTLLRLADQRDQSCAEVDGIAREHLQVVERKIADL
                     EVLRGELSQLIAFCGQGNIAQCRILQALTPAQAATE"
     gene            1469..>2297
                     /locus_tag="VFN42_12570"
     CDS             1469..>2297
                     /locus_tag="VFN42_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HET8997493.1"
                     /translation="MPDAVAAATSGATPLRYRVSGMDCPSCAGKIETALRRLDGTDDI
                     RINFHTQVLTVRLDEAATSRDRVEASIRQLGFGVASADLRAVAPDAVSEGSASEAVPA
                     WQAWLRLPKTRLAAAIGLLLVAGFAVTWVQPGIGHWAYLPAALLGLAFFGRRAMTLAR
                     AGSPFSIEMLMSIAALGAILIGATTEAAAVVFLFSIGELLEGVAAGRARAGIHALESL
                     LPRTALLVADGAARPVRTGSLQPGDLLLIRPGDRVPADGTIADGESELDEGPITGESI
                     "
BASE COUNT          390 a          832 c          739 g          336 t
ORIGIN      
        1 ggcatgacaa aatgagtttg cgtagaaccc gccgcggccc tgtgcgctga cgccatggcc
       61 tgtaccggca ccccggacca cagcaggcca acggcgacca gtatcgaagc aacaagccgt
      121 acgatgctaa cgagccgagc cggcatccga caccaaaaga agattacgca gtcgggtttc
      181 ctgaagtcgg gaccagttaa ccagccgggc actctccggc attgatctgg gtcaacggcg
      241 gcgctgccca tcgtcggcaa cgatgcggat cacttcccca tcaattcgct gatcaccgcc
      301 atcgccagaa acccgacgaa gaacgcgcca gcaccccagg cggtctccgg ccgttcgtgc
      361 gcctcggtga gcagttcctc cgtcaccaga tacagcagtg cgatcagccc gaaggcgaag
      421 gccaccgcct gccaggtatg cggcaggccg ccgatcggct gtgcgatcag cacgccaagc
      481 ggcacgccca acgacacgcc gatcgtggtg ccgatcacgg tccatcgacc cgcgtctttc
      541 ccgaacgagc ccgcgatgga taggccgagg aacaggaact cgaccgccag cgcgatcgcc
      601 agcaacgtgc cctggcgctg gccggcattg aagccaagcc ccagcaccag gccatcgatg
      661 agcgcatcga cggcggaggc ggccaccagt cccacagggc tttcttccgc accctccgtg
      721 agatgccgaa gcagcagcat gcccgcgatg ccgaccacac cgccagacac cagcgtccaa
      781 accgcgcctt ggtggatcgc atccggcagg atttcgcctg ccgccgcata gaacaccacg
      841 ccggccgcga ggtgctgcac cgcgcccact agtttcggcg taggctcccg ccagcttgtc
      901 caggccgcac caaggctggc cacgatcacc gggatgaagg cgtagaacca cgtcatgccg
      961 caggtgccag cgtcgtcatt cggtcgccgc ctgcgccggc gtcagcgcct gaaggatccg
     1021 gcactgcgcg atgttgccct ggccacagaa cgcgatcagc tgcgacaact ccccccgcag
     1081 cacctccagg tcggcgatct tgcgctccac cacctgcagg tgctcccgcg cgatgccgtc
     1141 cacctcggcg caggactggt cccgctgatc ggcgagccgc agcagcgtgc gcacctgatc
     1201 cagcgagaac cccaattcgc gcgcccgccg gatgaagctg agccgcgcca ggtgcgccgg
     1261 tccgtagcta cggtagttgc cggcggtccg ttccggctgt ggcaacaggc cgacccgctc
     1321 gtaccagcgc acggtttcca ccttgatgcc ggtcgccttg gcgaccgcgc cgatacccaa
     1381 ggactccgtc atggcttgac cctgtagtga ctacaggccc catctgggtt ggcatccgct
     1441 ccaggcgcaa cccgaatgga ggtcacacat gccagacgcc gtcgcggcag ccacgtcggg
     1501 cgcaacgccg ttgcgctacc gtgtgtccgg catggattgc ccaagctgcg ccggcaagat
     1561 cgaaacggcg ctccgccgcc tggacggcac ggacgatatc cggatcaatt tccacaccca
     1621 ggtgctgacg gtgcgcctgg acgaggccgc cacatcccgc gaccgggtgg aggccagcat
     1681 ccgccagctc ggcttcggcg tcgcgtcggc cgatctgcgc gcggtcgccc ccgacgcggt
     1741 atccgaaggc agcgccagcg aggcggtccc cgcctggcag gcatggctcc ggctgccgaa
     1801 gacacggctg gcggctgcca tcgggctgct gctggtggcc ggtttcgcgg tgacgtgggt
     1861 ccagccaggc atcggccact gggcctatct gccggccgcc ctgctcggcc tggcgttctt
     1921 cggccggcgg gcgatgacgc tggcgcgtgc cggctcgccg ttctcgatcg agatgctgat
     1981 gtcgatcgcc gcgctgggcg ccattctgat cggcgccacg accgaagcgg cggctgtggt
     2041 gtttctgttc agcatcggcg agttgctgga gggcgtcgcc gccggccggg cacgggcggg
     2101 tatccacgcg ctggaatcgc tgctgccgcg caccgcgctg ctggtagccg acggcgccgc
     2161 gcggccggtg cgcacgggca gcctgcagcc cggcgacctg ctgctgatcc gcccaggcga
     2221 ccgggtgccc gcggatggca cgatcgccga cggcgaatca gagctggacg aaggcccgat
     2281 caccggcgag tcgatcc
//
LOCUS       DASSZH010000580         3340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_201634, whole genome shotgun sequence.
ACCESSION   DASSZH010000580 DASSZH010000000
VERSION     DASSZH010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201634"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VFN42_12575"
     CDS             <1..377
                     /locus_tag="VFN42_12575"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HET8997494.1"
                     /translation="AELSLIYGHTLRLALGFAVHLIGWIGTGFAGWLAYRAVGVPINF
                     DTALGIEALLAAVAAVSFLVPINAGVQEAGYAGLGAIFGIPAEMSLAVSLLRRARDLS
                     IGLPVLLIWQFVEIKRLRETPG"
     gene            418..1032
                     /locus_tag="VFN42_12580"
     CDS             418..1032
                     /locus_tag="VFN42_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET8997495.1"
                     /translation="MDFYERFVLPRLLAWAMRQKPLVPYRQRAAGSARGRVLELGIGS
                     GCNLPFYGPDVHSLIGIDPSRALLDITQGRRSDARFPLDLLQADAADLPLEAHSIDTV
                     LTTWTLCSVGDPLHVLSEARRVLKPDGRLIFVEHGRAPDAGVRRWQDRITPAWRRIAG
                     GCHLNRPVDTLVAAAGFTAAGLRTGYMRGPRIATFMYEGEAAPR"
     gene            complement(1039..1704)
                     /locus_tag="VFN42_12585"
     CDS             complement(1039..1704)
                     /locus_tag="VFN42_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997496.1"
                     /translation="MRPNCLVSSVFAATLVALLPGYASQAASPSDGGTMMTQQAPPPA
                     AQSGAAQSQAGDEGGPGFQPGMRGGNGPGVRGGYGPGYGGWQGYGDGPGWMRGDGYGP
                     GWRHGDWPGWMHGYGPGMMYGYGPGMMAGCLPWMGPQFGGGPWGYHRSYHPAALNLSI
                     QDVRNRFERWLQWRHNPHLKLGEVRQTGPDTITADIVTQDNSLVQRFNVDRRSGQMYP
                     ADN"
     gene            1864..2355
                     /locus_tag="VFN42_12590"
     CDS             1864..2355
                     /locus_tag="VFN42_12590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HET8997497.1"
                     /translation="MTEMTAASKPHPWLRRLLRAPAWLYRCRCGWLLGHRFLLLIHTG
                     RRSGLRRCTVLEIAEYRPQMPEAVVVSGWGRGADWLRNLAATPQPEVIIGTHRFPAAS
                     RLLGREEAIAVIHGYERRNRWMLPVIHYVLSRLLGWRYDGSAQSRRRLAEQLPLVAFR
                     PAA"
     gene            2355..3083
                     /locus_tag="VFN42_12595"
     CDS             2355..3083
                     /locus_tag="VFN42_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HET8997498.1"
                     /translation="MADTEPAPRTDGAAGEASLEPRIVITREGLRDGSLIADARRRMP
                     AGIRLLSDAEIEADLDAKLAEHPPGADVWLFGYGSLMWNPAIDYAEHRAGAVRGWHRR
                     FCLWLHMGRGSPDNPGLMLALDRGGSCAGMLFRIPAAEARSELLLAWRRELFSGAYRS
                     RWVTAQTDTGPVRAATFVVNRAHPRYAGRLEEPAVAERLASAAGELGSCAAYLRATLG
                     ALGELGLRDRGLERLQRLVVQQAG"
     gene            complement(3129..>3340)
                     /locus_tag="VFN42_12600"
     CDS             complement(3129..>3340)
                     /locus_tag="VFN42_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997499.1"
                     /translation="EEAARGLRELGMQGRRFGNAAVYDALLRQTTEAVKAIVPGSALS
                     LADKTRLVEILAGPDEAWAVLGQPV"
BASE COUNT          469 a         1180 c         1120 g          571 t
ORIGIN      
        1 aggcggagct gtcgctgatt tatggccata cgctgcgcct ggcgctgggt tttgccgtgc
       61 acctgatcgg ctggatcggt accggctttg ccggctggct ggcctatcgc gctgtcgggg
      121 tgccgatcaa ctttgacacc gccctcggca tcgaggcgct gctggcagcc gtcgccgcgg
      181 tgtccttcct ggtgccgatc aacgccggcg tacaggaggc gggttacgcc gggctcggcg
      241 cgatcttcgg cattccggcg gaaatgtcac tcgccgtttc cctgctgcgc cgcgcccgcg
      301 acctgtcgat cggccttccc gtgctgctga tctggcaatt tgtggagatc aagcgcttgc
      361 gggagactcc aggctagact gctggtcgga acgcggctcg atcgcgaggg gcgcggcatg
      421 gatttctacg agcgtttcgt tctgccgcgc ctcctcgcct gggcaatgcg tcagaaaccc
      481 ctggtgccat accgccagcg tgcggcgggc tccgccaggg gacgcgtgct ggaactcggc
      541 atcggctccg gctgcaacct gccgttctac ggaccggacg tgcactcgct gatcggcatc
      601 gacccgtcgc gtgcactgct ggacatcacc cagggcaggc gatcggacgc acgctttccg
      661 ctggacctgc tgcaggcgga tgcagcggac ctgccgctgg aggcgcacag catcgacacc
      721 gtgctgacca cctggacgct gtgcagcgtc ggcgatccgc tgcacgtgct gagcgaggcg
      781 cgccgcgtgc tgaaaccgga cgggcggctg attttcgtcg aacacgggcg ggcgccggac
      841 gccggtgtcc gccggtggca ggatcggatc acgccagcct ggcggcggat cgccggcggc
      901 tgccatctca atcggccggt cgatacgctg gtcgccgccg ccgggttcac cgccgccggc
      961 ctgcgcaccg gctatatgcg cggaccccgc atcgcgacgt tcatgtacga gggcgaagcc
     1021 gcaccgcgtt gaacccggtc agttgtcggc cggatacatc tgtccggacc ggcgatcgac
     1081 attgaagcgc tgcaccagcg agttgtcctg ggtgacgatg tcggcggtga tggtatccgg
     1141 tccggtctgc ctgacctcgc cgagcttcag atgcgggttg tggcgccatt gcagccagcg
     1201 ctcgaaccgg ttgcgcacat cctgaatgct caggttcagc gctgccggat gatagctgcg
     1261 gtggtagccc cagggcccgc cgccgaattg cggccccatc cagggcaggc atccagccat
     1321 catgcctggc ccatagccgt acatcatccc gggaccgtag ccgtgcatcc agcccggcca
     1381 atcgccatgc ctccagccgg gcccgtagcc atcgccgcgc atccagccag ggccatcgcc
     1441 gtagccctgc cagcctccat agcccgggcc gtagccgccg cgcacccctg ggccattgcc
     1501 gcccctcatc ccgggctgga accccgggcc gccctcgtca ccggcctggc tctgtgccgc
     1561 cccggactgg gcggccgggg gcggggcttg ctgcgtcatc atcgtgccgc cgtccgacgg
     1621 cgaggccgcc tgggacgcat agccgggaag caacgcgacc agcgttgcgg caaaaaccga
     1681 gctgacaagg caattcgggc gcatcacagc gtctccctgc tgttggagcg gtcaggtctc
     1741 ccggtcagac tgcatcttgg cccgcatcag aacattgatg caggtcaaac ctgagctttg
     1801 cagatcttcc cggcctgctc gtgctggcaa aggtcttcac gccggcgcgg tatgatgctt
     1861 tgcatgacgg aaatgaccgc cgcatcgaaa ccgcacccct ggctgcgccg cctgctccgc
     1921 gccccggcct ggctgtaccg ctgccgctgc ggctggctgc tcggccaccg cttcctgctg
     1981 ctgatccaca ccggccggcg cagcggattg cgtcgctgca cggtgctgga aatcgccgaa
     2041 tatcgcccgc aaatgccgga ggccgtggtg gtcagcggct ggggccgcgg tgccgactgg
     2101 ctgcgcaacc ttgcggcaac gccgcagccg gaggtcatca tcggcacgca ccgttttccc
     2161 gccgcctccc gtctgctcgg ccgggaggaa gccatcgcgg tgatccacgg ttacgagcgt
     2221 cgcaaccgat ggatgctgcc ggtcatccac tacgtgctca gccgcttgct gggctggcgc
     2281 tatgacggat cggctcagtc gcgtcggcgg ctggccgaac agcttcccct ggtcgcattc
     2341 cgtccggcgg cgtgatggcg gacaccgaac cggcgccccg gaccgatggt gctgcaggcg
     2401 aagccagcct ggagccccgc atcgtcatca cccgcgaggg actgcgcgac ggttcactca
     2461 tcgccgatgc acgccgccgc atgccggcgg gaatccgcct gctgtccgat gcggagatcg
     2521 aagcggacct tgacgcgaag ctcgccgagc atccgcccgg agccgacgtg tggctgttcg
     2581 gctacggctc gctgatgtgg aaccccgcca tcgactacgc ggaacaccgc gccggcgccg
     2641 tgcgcggctg gcaccggcgc ttctgcctgt ggctgcatat gggccgcggc tcgccggaca
     2701 atcccgggct gatgctggca ctggaccgcg gcggaagctg cgccggcatg ctgttccgta
     2761 tcccggccgc cgaggcacgc tcggaacttc tgctggcctg gcgccgcgag ctgttttccg
     2821 gggcttatcg ctcccgctgg gtgaccgcgc agaccgatac cgggccggtg cgggccgcca
     2881 cctttgtcgt caaccgtgcg cacccgcgtt acgcgggccg cctggaggag ccggcagtgg
     2941 ccgagcgtct cgcctcggca gcgggggagc ttggcagttg cgccgcttat ctgcgcgcga
     3001 cgctgggcgc actgggagaa ttggggttgc gcgatcgtgg gctggaacga ctgcaacggc
     3061 tggtggtgca acaggcgggg tgacgcctgg gacaccctcc tcccaccagc cagccgtcct
     3121 tctttcgcct acacgggttg ccccagcacc gcccatgctt cgtccgggcc ggccaggatc
     3181 tcgaccagcc gcgtcttgtc cgccagcgac agggcactgc cagggacgat cgccttcact
     3241 gcctcggtgg tttgccgcag cagcgcgtca tagacggccg cgttgccaaa tcggcggccc
     3301 tgcatgccga gctcccgcaa gccgcgcgca gcctcctcca
//
LOCUS       DASSZH010000581         2148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_695446, whole genome shotgun sequence.
ACCESSION   DASSZH010000581 DASSZH010000000
VERSION     DASSZH010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695446"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2148)
                     /locus_tag="VFN42_12605"
     CDS             complement(<1..>2148)
                     /locus_tag="VFN42_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997500.1"
                     /translation="AILQANGGTLAQSGTITAITGGSLAASGNLTNSGLVSVSAAGGS
                     VALTSTGADIGTSGTIQTTGSASTVNLTAKGSVNQTAGLIAAGTPNDTTTAGTPSVTL
                     LAQTGTIGQSAGTILANNNAGTISLTAQAAGINTAGSIEAAGSAGTISLLAYSSIVQS
                     GGTILSGAPGLIAVSGSATPSLSLIAQHGSIEQDAGALIETTNTAGVIGLQAAGDITF
                     AGSIMTPGSSMNGTVVVLAPIGGVWLTSSGGSITEDSAHGYLQTGTLIGSANGSAALT
                     GSPDNKVATLGSFTVGNSFTLADAQSLSVVAPVTANTGSVVISEPGSLSSGSLTNQSV
                     IQGQSGVTLTTKDTLTNAGTIKAVTNDVSLTATATLGNSGLVDAANNAVLVVGGNLLN
                     SGTIQAQAGNAALTSTFQTVSNSGLVSAGHDAVLTAGADIDNASGATIAAGHFATLTA
                     AGSVNDVGLITVAGSGAAGTPSLQLIATGGTIDETGSGVIAASNNAGVVALTTKSGRN
                     ITLAGQVLAIGSSGLVTIAAGGAMTQTGGTIIAGGAGTSGTPSLAITANGGTIGQTAG
                     TIIASNASGLIALNASSDIDTAGSIGATGSAGVVAMNALGSINQTAGLIQSNGSGSIA
                     ASGALSSPNLTLNADGGSITQSAGAVIYAPNGAGVILLRANHDIGLNGSVLAPGAHGG
                     IALASANGSLLQSAGTGILLANTLIGSAAQTVA"
BASE COUNT          296 a          720 c          760 g          372 t
ORIGIN      
        1 gcgcgaccgt ctgcgcggcg gacccgatca gcgtgttcgc cagcagaatg cccgtgccgg
       61 cggactgcag cagcgagccg ttcgccgagg ccagggcgat tccgccatga gcgcccggcg
      121 ccagcaccga tccgttcagc ccgatgtcgt ggtttgcgcg cagcaggatc acccccgctc
      181 cgttcggcgc gtagatcacc gcgccggcac tctgggtgat cgagccgcca tccgcgttga
      241 gtgtcagatt cggtgagctg agcgcgcccg acgccgcgat gctcccgctg ccgttggact
      301 ggatcaggcc cgccgtctgg ttgatcgaac ccagcgcgtt catcgccacc acgccggcgc
      361 tgccggtggc gccgatgctg ccggccgtgt cgatgtccga actggcgttc agggcgatca
      421 ggccgctggc attgctcgcg attatagtgc ccgcggtctg cccgatcgtg ccgccgttcg
      481 cggtgatcgc caggctcggc gtccccgatg tacccgcgcc gccggcgatg atcgtgccgc
      541 cggtctgcgt catcgcgccg cccgcggcga tggtgaccag gcctgagctg ccaatcgcca
      601 gcacctggcc ggccagcgtg atgttgcggc cgctcttggt cgtcaacgcg accacacccg
      661 cgttgttgga tgcggcgatc acgccggaac ccgtctcatc gatggtgccg cccgtcgcga
      721 tgagttgcag gctgggcgta cccgccgcgc cggagccggc cacggtgatc aatccgacat
      781 cgttgaccga tccggccgcg gtcaaggtgg cgaagtgtcc ggcggcgatc gtcgcgccag
      841 aggcattgtc gatatcggcg cccgcggtca gcaccgcgtc gtgcccggcg ctgaccaggc
      901 cgctgttgct cacggtctgg aaggtggagg tcagcgccgc gttcccggcc tgcgcctgga
      961 tggtgccgct gttcagcagg ttgccgccga ccaccagcac cgcgttgttt gccgcatcga
     1021 ccaacccgct gttgccgagg gtggcggtgg cggtcagcga gacgtcgttg gtgaccgcct
     1081 tgatcgttcc ggcattcgtc agcgtgtcct tcgtggtcag ggtaacgcca ctttgcccct
     1141 ggatgaccga ctggtttgtc agagaaccgg acgacaggct gcccggctcg ctgatcacca
     1201 ccgagccggt attggccgtc accggcgcca ccaccgacag cgactgcgca tcggccaggg
     1261 tgaagctatt gccgacggtg aaggagccaa gcgtcgccac cttattgtcc ggcgagccgg
     1321 tcagtgccgc ggagccattg gccgagccga tcagcgtgcc ggtctgcagg tagccgtggg
     1381 ccgagtcctc ggtgatcgaa ccaccgctgg aggtgagcca cacgccaccg attggcgcca
     1441 gcaccaccac ggtgccattc atgcttgagc cgggggtcat gatcgatccg gcaaaggtga
     1501 tgtcgccggc cgcctgcaag ccgatgactc cggcggtgtt ggtggtctcg atcagtgcgc
     1561 cggcatcctg ctcgatggaa ccatgctggg cgatcagcga caggcttggc gtggcgctgc
     1621 cggacaccgc gatcaggccg ggggcgcccg agaggatcgt cccgccggac tgcacgatgg
     1681 agctgtaggc cagcaggctg atggtaccgg cggagccggc agcctcgatg ctgcccgccg
     1741 tgttgatgcc ggcggcctgc gcggtcagtg aaatcgtccc cgcgttgttg ttcgccagga
     1801 tcgtaccggc ggactgaccg atggtgccgg tctgcgccag caaggtgacg ctcggcgtgc
     1861 cggcggtggt ggtgtcgttc ggagtgcccg cggcgatcag gccggcggtc tggttgaccg
     1921 agcccttcgc ggtcagattc accgtgctgg ccgacccggt cgtctggatg gtcccggaag
     1981 tgccgatgtc ggcaccggtc gaggtcagcg ccacggagcc gccggcggcg gacactgaca
     2041 cgaggccgct gttggtcagg ttgccgcttg cggccagcga cccgccggtg atcgcggtga
     2101 tggtgccgga ctgggccagc gtgccgccat tcgcttgcag gatggcgg
//
LOCUS       DASSZH010000582         4917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1514131, whole genome shotgun sequence.
ACCESSION   DASSZH010000582 DASSZH010000000
VERSION     DASSZH010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514131"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VFN42_12610"
     CDS             complement(<1..639)
                     /locus_tag="VFN42_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HET8997501.1"
                     /translation="MSWLSNFLQKHWVEWLLAAILLSVIVWFFGPLLGLGALHPLASA
                     LGRGIAIAAIFVLWLIAVLVHELHEHKKEKELEKDVAEAASEHDPAATASAEEVALLS
                     ERMKEALHQLKRAKLGGRSRRHLYQLPWYMFIGPPGAGKTTALVNCGLNFPLADEHGP
                     KAIQGVGGTRNCDWWFTDQAVLIDTAGRYTTQDSNTAVDAAAWFGFLKLLKKH"
     gene            complement(636..1982)
                     /gene="tssL"
                     /locus_tag="VFN42_12615"
     CDS             complement(636..1982)
                     /gene="tssL"
                     /locus_tag="VFN42_12615"
                     /inference="COORDINATES: protein motif:HMM:NF038228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, long
                     form"
                     /protein_id="HET8997502.1"
                     /translation="MSDDPFAEPSDVEATIIRPVPGGRSGSAAAPRPAAARPQPAAGT
                     VPTVGANPLLAAAAPLLAAAIRIGAGRGRNPDPEQLRRGMVEAVREFEKRALATGLDT
                     RSLRAARYALCATIDDLVLSTPWGSASTWAAQSLTSIFHNEVSGGERFFDILQDMEKD
                     LGRHSPVVELMYLCTSLGFEGRYRVMPRGIAALTELRDSIYRTIRQRRGDFERELSPR
                     WRGLETGYKPLVQRVPLWWLAVGTLGMACLIYLFFDFSLAAASDVAFSEMAGLPPHGA
                     ISVPRTVVVPPPPPPSPEVVAAASPAEPKFSQFLAPEIKQGLVKVFEDPQSITVRLIN
                     RNMFASGSATLSHSYLPLLDRIGAELQKSQGNILINGYTDNQPIHTVQFPSNWQLSQA
                     RADAVASIIKSKLADPTRVRVVGKGDTDPIASNATPQGRELNRRTEIVLMKAAKPL"
     gene            complement(1990..3327)
                     /gene="tssK"
                     /locus_tag="VFN42_12620"
     CDS             complement(1990..3327)
                     /gene="tssK"
                     /locus_tag="VFN42_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HET8997503.1"
                     /translation="MGWENKVVWMEGLFLQPQHFQQQDRYVERLVRSSTAGLRPFAYG
                     LTQLELDSDLLALGKFALRAAAGILPDGTPFSIPGDVEHPQPIDVPETARNGAVYLIL
                     PARQPGGVAAAPADRVETVARFIIGEHEAADTNAGFQNAAILPVGQLRLRYALEGEAR
                     EGFTGIGLARIAEVRPDKSVQLDDAYIPPLLLTSASPTLAGFVTQLQGLLHHRAEALA
                     GRVSETATRGAAEIADYLMLQLCNRYEPLLTHLAGTVNQLHPETLYRACVSLAGELAT
                     FTESRKRPAEFPTYRHDDLQATFRPVIAALRQSLSAVLEQNAVPIPLQERKFGIRVGP
                     IADRTLVASATWVLAVKAQMPAEALRRGFPNLVKIGPVEQIRELNNVALPGGAVRALP
                     VAPRQLPYYAGTTYFELDRSSPYWAALARSGGIAIHVTGDVPGLEIECWAIRG"
     gene            complement(3330..3797)
                     /gene="tssJ"
                     /locus_tag="VFN42_12625"
     CDS             complement(3330..3797)
                     /gene="tssJ"
                     /locus_tag="VFN42_12625"
                     /inference="COORDINATES: protein motif:HMM:TIGR03352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HET8997504.1"
                     /translation="MGRWLPGAAACALLAVLAGCSPPPPPPPTVVNLTLSATPDANPD
                     QSGQGAPVAIRVYQLSSTSAFDTAEFFPLFNNDTGTLKDTLVQRDQFILAPGKSKTVT
                     LTPKDQVKSLGFFAAYRDFQHAQWRGELDVPPHKTTTVTVTAGKAGITVKSGP"
     gene            complement(4022..4354)
                     /locus_tag="VFN42_12630"
     CDS             complement(4022..4354)
                     /locus_tag="VFN42_12630"
                     /inference="COORDINATES: protein motif:HMM:NF027074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HET8997505.1"
                     /translation="MASPKKPASHKRADPPRTADYTKLFFKDWERLTRSGRYNMQHLK
                     QAMLLLIANNGPLGTEWRDHALAGNWADHRECHVGGDFLLIYRIIGDGPQGAVVFVRA
                     GTHAELFE"
     gene            complement(4329..4595)
                     /locus_tag="VFN42_12635"
     CDS             complement(4329..4595)
                     /locus_tag="VFN42_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HET8997506.1"
                     /translation="MPATTMLHVRVDEETKEQATAALEAMGLSLSDAVRVFLRRVAVE
                     QAIPFPIKVPNAETRAAMEEARAMSGGRFRTPEALFDGLAKETR"
     gene            complement(4721..>4917)
                     /locus_tag="VFN42_12640"
     CDS             complement(4721..>4917)
                     /locus_tag="VFN42_12640"
                     /inference="COORDINATES: protein motif:HMM:NF042526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein"
                     /protein_id="HET8997507.1"
                     /translation="EPSALEKRLQPGMLGSVLPSSRKARTWELFCETYKDIAREAEDD
                     FQSVFGREFARAYDAQMRKL"
BASE COUNT          731 a         1529 c         1816 g          841 t
ORIGIN      
        1 gtgtttcttc agcagcttga ggaagccgaa ccaggcggcg gcatccaccg cggtgttgga
       61 atcctgagtg gtgtagcggc cggcggtatc gatcagcacc gcctggtcgg tgaaccacca
      121 gtcgcaattg cgggtgccgc cgacgccctg gatggccttg gggccgtgct cgtcggccag
      181 cgggaagttc aggccgcaat tgaccagcgc ggtggttttg ccggcgccgg gcgggccgat
      241 gaacatgtac cagggcagtt ggtacagatg gcgccgggag cggccgccga gcttggcgcg
      301 cttgagctgg tgcagcgcct ccttcatgcg ttcgctcagc agcgcaactt cctcggccga
      361 ggcggtggcg gccgggtcat gctccgacgc cgcttccgcg acgtccttct ccaactcctt
      421 ttccttcttg tgctcgtgca gctcgtgcac cagcacggcg atcagccaga gcacgaaaat
      481 cgcggcgatg gcgatgccgc ggcccaaggc ggaggcgagc ggatgcagcg cgccaagacc
      541 gagcagcggg ccgaagaacc agacgatgac agagagcagg atggcggcga gcagccactc
      601 gacccaatgc ttctggagga aattgctcag ccagctcata ggggtttcgc cgccttcatc
      661 agcacgatct cggtgcgccg gttgagttcg cgtccctgtg gcgtggcgtt gctggcgatc
      721 gggtcggtat cgcccttgcc gacgacgcgt acgcgcgtgg ggtccgccag tttcgacttg
      781 atgatgctgg ccacggcatc ggcgcgggcc tgggacagtt gccagttgga cgggaactgc
      841 acggtgtgga tcggctggtt gtcggtgtag ccgttgatca ggatgttacc ctgggatttc
      901 tgcagttcgg cgccgatgcg atccagcagc ggcaggtagc tgtggctgag cgtggcggag
      961 cccgaggcga acatgttgcg gttgatcagc cgcacggtga tggactgcgg atcctcgaac
     1021 accttgacca ggccctgctt gatctccggc gcgaggaact ggctgaactt tggttcggcg
     1081 ggactggcgg cggccaccac ctccggcgag ggtggcggcg gcggcgggac cacgacggtg
     1141 cgcggtacgc tgatggcgcc gtgcggcggc aggccggcca tttcgctgaa ggccacgtcg
     1201 gacgcggcgg cgagggagaa gtcgaagaac aggtagatca ggcaggccat gcccagcgtg
     1261 ccgacggcga gccaccacag cggcacgcgc tgcaccaacg gcttgtagcc ggtttccagg
     1321 ccgcgccagc gcggcgacag ctcacgctcg aaatcgccgc ggcgctggcg gatggtgcgg
     1381 tagatcgaat cgcgcagctc ggtcagcgcg gcgatgccgc gtggcatgac gcggtagcgc
     1441 ccttcgaaac cgagggaggt gcagagatac atcagctcga ccacggggct gtggcggccg
     1501 aggtccttct ccatgtcctg caggatgtcg aagaagcgtt cgccgccgct gacctcgtta
     1561 tggaagatgc tggtcaggct ctgcgcggcc caggtgctgg cgctgcccca gggggtgctg
     1621 agcaccaggt cgtcgatggt ggcgcacagg gcgtagcggg cggcgcgcag gctgcgggta
     1681 tccaggccgg tggccaaagc gcgcttctcg aattcgcgca ccgcctcgac catgccgcgg
     1741 cgcagctgct cggggtcggg gttgcggccg cggccggcgc cgatgcggat ggcggcggcg
     1801 agcagcgggg cggcggcggc gagcagtgga ttggcgccga ccgtgggcac cgtgccggcg
     1861 gccggttgcg ggcgtgcggc ggctgggcgg ggtgcagccg cggagccgct gcgtccgccg
     1921 ggtaccgggc ggatgatggt ggcttcgacg tccgacggct cggcgaaggg atcatcactc
     1981 atcggctgct caccctctga tcgcccagca ttctatttcc aggcccggca cgtcgcctgt
     2041 gacgtggatc gcaatgccgc ccgaacgggc gagcgccgcc cagtacgggc tgctgcggtc
     2101 gagctcgaaa tacgtggtgc cggcgtagta cggcagctgg cgcggcgcga cgggcagcgc
     2161 gcgcacggcg ccgccgggca gggcgacgtt gttcagttcg cggatctgct cgaccgggcc
     2221 gatcttcacc aggttgggga agccgcggcg cagcgcctcg gccggcatct gcgccttgac
     2281 cgccagcacc caggtggcgc tggccaccag ggtgcggtcg gcgatcggtc cgacgcggat
     2341 gccgaacttg cgttcctgca gcgggatcgg cacggcgttc tgttccagca cggcggacag
     2401 cgactggcgc agcgcggcga tcaccgggcg gaaggtcgcc tgcaggtcat cgtgccggta
     2461 ggtggggaat tcggctgggc gcttgcggga ttcggtgaag gtggcgagtt cgccggccag
     2521 gctgacgcag gcccggtaca gggtttccgg gtgcaactgg ttcaccgtgc cggccagatg
     2581 cgtcagcagc ggctcgtagc ggttgcacaa ctgcagcatc aggtagtcgg cgatctccgc
     2641 ggcgccgcgg gtggcggttt ccgacacgcg gccggccagc gcctcggcgc ggtgatgcag
     2701 caggccctgc agctgggtga cgaagccggc cagcgtgggg gaggcgctgg tcagcagcag
     2761 cggcggaata taggcatcgt cgagctgcac gcttttgtcg gggcgcactt cggcgatgcg
     2821 ggcgaggccg atgccggtga agccttcccg tgcctcgcct tccaacgcgt agcgcaggcg
     2881 gagctggccg acgggcagga tggcggcgtt ctggaagccg gcattggtat cggcggcctc
     2941 gtgctcgccg atgatgaagc gcgccacggt ctccacccgg tcggccggcg cggcggccac
     3001 gccgccgggc tggcgggcgg gcaggatcag gtacaccgcg ccgttgcggg cggtctccgg
     3061 cacgtcgatc ggctgcgggt gctcgacgtc gccgggaatg ctgaagggcg taccgtccgg
     3121 caggatgccg gcggcggcgc gcagggcgaa cttgcccagg gccagcaggt cggaatcgag
     3181 ttccagctgg gtcaggccat aggcgaaggg gcgcagcccg gcggtgctgc tgcgcaccag
     3241 gcgttcgacg tagcggtcct gttgctggaa gtgctgcggc tggaggaaca gcccctccat
     3301 ccagacgacc ttgttctccc agcccatggt caggggccgg acttgacggt gatgccggct
     3361 ttgccggcgg tgacggtcac cgtggtggtc ttgtgcggcg ggacatcaag ctcgccgcgc
     3421 cattgggcgt gctggaagtc gcggtaggcg gcgaagaagc cgagtgactt gacctggtcc
     3481 ttcggggtca gggtgacggt cttgctcttg ccgggggcca ggatgaactg gtcgcgctgg
     3541 accagggtgt ccttcagcgt gccggtgtcg ttgttgaaca gcgggaagaa ctcggcggta
     3601 tcgaaggcgc tggtggaact gagctgatag acgcggatcg ccaccggggc gccttgtccg
     3661 gactgatcgg ggttggcgtc cggggtcgcg ctcagggtga ggttgaccac ggtcgggggg
     3721 ggcggcggcg ggggcgagca gccggcaaga acggcgagca gggcgcacgc cgctgctcca
     3781 ggaagccatc gacccatgct gttcagtctc ccccaacgcg gcggcctgtc tgcccccgct
     3841 gcatcggttg tggcacgccc cgtcgcgtgg tgttgccgat agtggcttct atacacgagc
     3901 gcgcgggtgt ccggaatttt ctattgagcc ggaggtgcgc caatgcgccc ttttgccgtc
     3961 agtttggcag ccctgcgccc aacctggtac ctgccggctc cacgatcggt ccgcgcggcg
     4021 gtcactcgaa cagctcagcg tgcgttccgg cccggacgaa taccacggcc ccttgcggcc
     4081 catcaccgat gatgcggtag atcagcagga aatcgccacc tacgtggcac tcccgatgat
     4141 ccgcccaatt cccggccaga gcatggtctc gccactcggt cccgagcggt ccattgttcg
     4201 cgatcaacag cagcatggcc tgcttgaggt gctgcatgtt gtatcggcct gagcgcgtca
     4261 gccgctccca gtccttgaag aacagcttgg tgtaatccgc tgttcgcggt ggatcggcgc
     4321 gcttatggct agcgggtttc tttggcgagg ccatcgaaca gagcctcggg ggtgcggaag
     4381 cgaccgccgc tcatcgcgcg cgcctcctcc attgccgcgc gagtctccgc gttcggcacc
     4441 tttattggga agggaatcgc ctgctcgacg gcaacgcggc gcaggaagac acgcacagca
     4501 tccgagaggg acagtcccat tgcctcaagg gcggcggtgg cctgctcctt ggtttcttcg
     4561 tcgacgcgga cgtggagcat cgttgtggcc ggcatgggcg accctttccg aacggggaag
     4621 ttcgtatctc acctgagata caccaacggc gccctgctgc aaggggggct tcttggtttt
     4681 acagatcaac cgttcaactg gctttcgcca ccaggtccgc tcagagtttc cgcatctgcg
     4741 cgtcgtaggc gcgggcgaac tcgcggccga acactgactg gaagtcgtcc tccgcctcgc
     4801 gggctatgtc cttgtaggtc tcgcagaaca gctcccaggt gcgcgccttg cggctggacg
     4861 gcagcacgct gcccagcatg ccgggctgca gccgcttctc cagcgcggac ggctcga
//
LOCUS       DASSZH010000583         3301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1480038, whole genome shotgun sequence.
ACCESSION   DASSZH010000583 DASSZH010000000
VERSION     DASSZH010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480038"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="VFN42_12645"
     CDS             complement(<1..1073)
                     /locus_tag="VFN42_12645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET8997508.1"
                     /translation="MQFSFTDEQQEFRSVLRRFLEDKSPPSVVRRLMQTESGWDRAAW
                     QELNQQLGLTAVHIPEEYGGQGFGAVELGIVLEEMGRALLCAPYFSSTVLAATAILHA
                     ATEAQKRALLPDIAAGETIATLAFTEPDGRWDTGGIACTATPDGSGYRLEGSKSFVLD
                     GHTADLIVVLARRPGSSGNDGLSFFTVASDAAGLSRRALKVMDPTRKLARLEFRSVRA
                     ELLGAEGGGAEPFAATMRLAAVGLANEMVGGAERLRQSALDYANLRVQFGRAIASFQS
                     MKHKQADMLVDVELAKSAAYAAASAAAENDPELPALASLAKACASEAYMQTAIHTIQI
                     HGGIGFTWDNDTHLWFKRAKSSEV"
     gene            1253..2104
                     /locus_tag="VFN42_12650"
     CDS             1253..2104
                     /locus_tag="VFN42_12650"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HET8997509.1"
                     /translation="MPRDSGLAITPAGAVGAHVTGLDLATPMDDADVGRLREALGEHG
                     VLFFRDQQLTPEQHIDAAARFAPINVNRFFACAPGYPAIAEVRKDPGQTKNIGGGWHT
                     DHSYDQLPALGSMLLARETPTRGGDTLFASMYAAYDALSDGLKQTLCGLQAWHSNRHV
                     FGAEAMQQRGDLKGRLQNPELATQDAVHPVVIRHPISGRPALYVNSGFTLRFDGWTAE
                     ESRPLLQYLYAHASKPEFTCRFAWQPGSIALWDNRATWHYALNDYHGERRLMHRITLE
                     GAALAAA"
     gene            2193..3287
                     /locus_tag="VFN42_12655"
     CDS             2193..3287
                     /locus_tag="VFN42_12655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8997510.1"
                     /translation="MDVGMMMVFASYGWENCSDTRVWDEEIRLARLAADLGFDCLWSA
                     EHHFNDYSFVPDNIQLMSYLTAACPDIDVGTAAVILPWHDPLRVAEQASVLDYLSKGR
                     FRFGMGRGLARREFAAFRVSMDESRGRFDEAAPMIINALKTGFIEGDGQYYKQPRIEI
                     RPRPQHDFTGRIYAVASSEDSVMSAARLGAHMVMFADRPWEMRMPVIEQGRALHRQFH
                     HSEPPAIMLTEFCVCGSDLAETEDEARRYQGKFVESNFYHYEFLGEHFKTVKGYDAYQ
                     QKAEIARKAGLDGAVNGFMQAASWGTPDKILRGLETRRTLLGEFELNVAFRFGGTPYS
                     VSERGLRLFAKEVLPVLKSWGPADAGKAAA"
BASE COUNT          571 a         1120 c         1052 g          558 t
ORIGIN      
        1 acttccgaac tcttcgcccg cttgaaccag agatgcgtgt cattgtccca ggtgaagccg
       61 atgccaccgt ggatctggat ggtgtggatc gcggtctgca tatacgcttc cgaggcgcag
      121 gccttggcca gtgatgccag cgccggcagc tcgggatcgt tctccgccgc cgcggatgct
      181 gccgcatagg ctgccgattt cgccagctcg acatccacca gcatgtcggc ctgcttgtgc
      241 ttcatcgatt ggaatgaagc gatggcgcgg ccaaactgca cgcgcagatt cgcgtagtcg
      301 agtgcggact ggcgcagccg ttcggcaccg ccgaccatct cgttcgccag cccaaccgcg
      361 gccagccgca tcgtcgcggc gaaaggttcc gcaccgccgc cttccgcgcc cagcagttcg
      421 gcccgcaccg aacggaattc cagccgcgcc agcttgcgcg tggggtccat caccttcagg
      481 gcgcggcgtg acagtcccgc cgcgtcgctg gcgactgtga agaacgacag cccgtcattg
      541 ccagacgaac ccggccggcg cgccagcacc acgatcaggt ccgcggtgtg gccgtccagc
      601 acgaagctct tgctaccctc caggcgatag ccggagccgt ccggcgtggc ggtgcaggcg
      661 ataccaccgg tgtcccaccg cccgtccggc tcggtgaacg ccagcgtggc gatggtttcg
      721 cccgccgcaa tgtccggcag caaggcccgc ttctgcgcct ctgtcgcggc atgcaggatg
      781 gcagtggcag cgagcacggt ggaggagaaa tacggcgcac atagcagggc gcgccccatc
      841 tcctccagca cgatccccag ttcgaccgca ccgaaaccct ggccgccata ctcctccgga
      901 atgtgcacgg cggtaagacc gagctgctgg ttgagttcct gccacgcggc acgatcccag
      961 ccggactcgg tttgcatcag ccgccgcacc accgacggtg gcgacttgtc ctccaggaac
     1021 cgccgcagga ccgagcggaa ctcctgctgc tcgtcggtga agctgaattg catgccgttt
     1081 gtctccggaa gcctcgccgg gcggtggaag ctgtcaccgc cattgaatgc cgttgccgat
     1141 gggtgcgcaa cccgccgggc ggttgccgcc agtgcaaggc cagggttagt tttgccgcgc
     1201 cgcgggttcg gccgctcgcc gcaatcaccg ccaatgagcc ggaggaacag ccatgccccg
     1261 cgacagcggt cttgccatca cgcccgccgg tgcggtcggc gcccatgtga ccgggctcga
     1321 cctcgccacg ccgatggatg atgcggatgt cggccggctg cgggaggcgc tgggggaaca
     1381 cggcgtgctg ttcttccgtg accagcaatt gacacccgag cagcacatcg acgccgccgc
     1441 ccggtttgcg ccgatcaacg tcaaccggtt cttcgcctgc gcgcccggtt atccggcgat
     1501 cgcggaagta cgcaaggatc ccgggcagac aaagaatatc ggtggcggct ggcataccga
     1561 ccacagttac gaccagcttc ccgcactcgg ctcgatgttg cttgcgcgcg agacgccgac
     1621 acgcggcggc gatacgctgt tcgccagtat gtacgccgct tacgatgcgt tgtccgacgg
     1681 gttgaagcag accctgtgcg gcctgcaggc ctggcattcc aaccgccacg tcttcggcgc
     1741 cgaggcgatg cagcaacgcg gtgacctgaa ggggcggctg cagaaccccg agctcgcaac
     1801 gcaggacgcg gtgcatccgg tggtgatccg gcatccgatc agcggccgcc ccgctctcta
     1861 cgtcaattcc ggtttcaccc tgcgcttcga tggctggacc gcggaggaaa gccggccgct
     1921 gctccagtac ctgtatgccc acgccagcaa accggagttc acctgccgat ttgcctggca
     1981 gccgggatcg atcgcgctct gggacaatcg tgccacctgg cactacgcgc tgaacgacta
     2041 ccacggggag cgccggctga tgcatcggat tacgctggaa ggagccgcgc tggccgctgc
     2101 atgaaggcgg caccgccctt gcgccacccc cctgtccgtg gaagcctttt ctccgaccgc
     2161 gcaccagcgt gaaccagcaa gggagaagcg ccgtggatgt cggaatgatg atggtgttcg
     2221 cgagctatgg ctgggagaac tgctccgata cgcgcgtgtg ggacgaggaa atccggctcg
     2281 cacgcctcgc cgccgatctt ggcttcgatt gcctgtggtc ggccgaacat cacttcaacg
     2341 actactcgtt cgtacccgac aacatccagt tgatgagcta cctgacggcg gcatgcccgg
     2401 atatcgacgt cggcacggcc gcggtgatcc tgccctggca cgatccgctg cgcgtcgccg
     2461 aacaggcgtc ggtgctggac tacctctcca agggccgctt ccgcttcggc atgggccgtg
     2521 gcctcgctcg gcgggaattc gccgccttcc gtgtcagcat ggatgaatcc cgcggccgct
     2581 tcgacgaagc ggcgccgatg atcatcaatg cattgaagac cggcttcatt gaaggcgacg
     2641 gccagtacta caagcagcca cgcatcgaga tccgcccccg cccgcagcac gatttcaccg
     2701 gccgcatcta cgcggtcgct tccagcgagg actcggtgat gtcagccgcc aggctcggcg
     2761 cgcatatggt gatgttcgcc gaccggcctt gggaaatgcg catgccggtg atcgagcaag
     2821 gccgggcgct gcatcgccag tttcaccaca gcgaaccgcc ggccatcatg ctgaccgaat
     2881 tctgcgtctg cggcagcgac ctcgccgaga ccgaggacga agcccgccgc taccagggca
     2941 agttcgtcga gagcaatttc taccattacg aattcctcgg cgagcacttc aagacggtga
     3001 agggctacga cgcctaccag cagaaggcgg agatcgcccg caaagccggt ctggacggcg
     3061 cggtgaacgg cttcatgcag gccgcgagct ggggtacgcc cgacaagatc ctgcgcggcc
     3121 tggaaacccg ccgcactctg cttggcgaat tcgagctgaa cgtcgccttc cgcttcggcg
     3181 gcacgccgta ctcggtctcg gagcgcgggc tgcggctgtt cgccaaggaa gtactgccgg
     3241 tgctgaaatc ctggggtccg gcggatgctg gaaaagcggc ggcctgatcg gattcactgc
     3301 g
//
LOCUS       DASSZH010000584         2609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_167448, whole genome shotgun sequence.
ACCESSION   DASSZH010000584 DASSZH010000000
VERSION     DASSZH010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167448"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VFN42_12660"
     CDS             complement(<1..188)
                     /locus_tag="VFN42_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HET8997511.1"
                     /translation="MRALDIAGTGMQAQQTNVEVISNNLANMTTTGFKRQRAEFQDLI
                     YQNLRRVGSNSSDAGTIVP"
     gene            complement(205..945)
                     /locus_tag="VFN42_12665"
     CDS             complement(205..945)
                     /locus_tag="VFN42_12665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HET8997512.1"
                     /translation="MDLISNIAASRSVAQQRTLDVIANNIANANTPGYQAERVQFSDW
                     ISPQSGTASPPGERSIAYAQDRATWREQQAGVLAHTANPLDLAVTGDGFFTVSTPRGP
                     RLTRDGRFGLMPNGTLADSAGNAVLDTAGQPIQFAPSDTHITVAGDGTVSSENGQIGK
                     IGIVRPTDPMRLTAEGNTLMRADAPTAPIAAPRIVQGAIAESNVQPVLEMTRMMDGLR
                     QFQALAQFMQAEGDRQQSAIDKLLPASP"
     gene            1246..1767
                     /locus_tag="VFN42_12670"
     CDS             1246..1767
                     /locus_tag="VFN42_12670"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HET8997513.1"
                     /translation="MAAAAPAPETAEPEAAAAEAESAKPASRLPRLLLLGVPLLLAAL
                     IAGLWFSGVLPRLLGFRQHAGTQAAAAPILPTYVDLPEIVANLNTAGRPSYVKLSARA
                     EVAGPKDAERVRQLMPRIQDLFQTYLREMRPDELRGSAGSYRLREELLARANIAVAPA
                     HINTVLFIQLLIQ"
     gene            1764..>2609
                     /gene="fliM"
                     /locus_tag="VFN42_12675"
     CDS             1764..>2609
                     /gene="fliM"
                     /locus_tag="VFN42_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HET8997514.1"
                     /translation="MSDSGKPPSDEELAAAWGAEEAAGDSAAQPARVLNQAEIDSLLG
                     FDDGPQGGENRTGMQKIISSGLVSYERLPMLEIVFDRLVRIMSTSLRNFTSDNVEVGI
                     DNILSLRFGDYLNSIPLPAMLAVFKAEEWDNDGLMVVDSAMIYSIVDVLLGGRRGTAA
                     MRIEGRPYTTIERTLVERLITVVLADLSASFDPLCPVTFRFERLEVNPRFATISRLSN
                     AAVLARLRVDMEDRGGRMELLLPYATLEPVRELLLQQFMGEKFGRDSIWETHLAEELW
                     NTEVEL"
BASE COUNT          383 a          859 c          914 g          453 t
ORIGIN      
        1 ggcacgatgg tgccggcgtc ggagctgttc gaccccaccc gccgcaggtt ctggtagatc
       61 aggtcctgga attcggcccg ctgccgcttg aagccggtgg tggtcatgtt ggcgaggttg
      121 ttggagatca cctcgacatt ggtctgctgg gcctgcatgc cggtgccggc gatgtcgagc
      181 gcacgcatgg gctggtgcct tcggtcaggg actggcgggc agcagcttgt cgatcgccga
      241 ctgctgccgg tcgccttccg cctgcatgaa ctgggccagt gcctggaatt gccgcaaccc
      301 gtccatcatg cgggtcattt ccagcaccgg ctgcacgttg gactccgcga tggcgccttg
      361 cacgatccgt ggtgcggcga taggtgcggt cggtgcatcg gcacgcatca gggtgttgcc
      421 ctcggcggtg agacgcatcg ggtcggtggg gcggacgatg ccgatcttgc cgatctggcc
      481 gttctcgctg gacacggtgc catcgccggc gacggtgatg tgcgtgtcgc tgggggcgaa
      541 ctggatcggc tgcccggcgg tgtccagcac ggcgttgccg gcgctgtcgg ccagcgtgcc
      601 gttcggcatc agcccgaaac ggccgtcgcg ggtcagccgc ggcccgcgcg gcgtgctgac
      661 ggtgaaaaac ccgtcgccgg tcaccgcaag gtccagcggg ttggcggtat gcgccagcac
      721 gccggcctgc tgttcccgcc acgtggcgcg gtcctgggcg taggcgatgc tgcgctcgcc
      781 gggcggtgac gcagtgccgc tctgcgggct gatccagtcg ctgaactgca cgcgctctgc
      841 ctggtagcct ggcgtgttgg cgttggcgat gttgttggcg atcacgtcca gcgtgcgctg
      901 ctgcgccacg gaacgcgatg cggcgatgtt gctgatcagg tccatgcggt gagagggagc
      961 aagctgcgtg ccgctgcagt ggaatccggg ctgtcttccg tcctgacacc ctcgtcatgg
     1021 ccggaaggcc ggccatccac gccttgctgt ggcggacccg cagcgcaatc ggggccagca
     1081 ccgccggact cccgcggcca ggcatggatg gccggccttc gccggccatg acgaggggga
     1141 acgccgatgc acggcagaat ttgccgggcg gcgcattccc ccgggtcggc aaggctttgt
     1201 taacctcgcc ttggcaccct ccgttccgcc gcaacaggaa cggacatggc cgccgccgcg
     1261 cctgcccccg agaccgctga accggaagcc gcagcagccg aagcggagtc ggccaagccg
     1321 gccagccgtc tgccgcgcct gctgctgctg ggcgtgccgc tgctgcttgc cgcgctgatc
     1381 gccggcctgt ggttctccgg cgtgctgccc cgcctgctcg gctttcgcca gcacgccggg
     1441 acgcaggcgg cggcagcgcc gatcctgccc acctatgtcg atctgccgga gatcgtcgct
     1501 aacctgaaca ccgcggggcg gccgagctat gtgaagttga gcgcgcgggc cgaggtcgcc
     1561 ggcccgaagg atgccgaacg cgtgcgccag ctcatgccgc gcatccagga cctgtttcag
     1621 acctatctgc gcgagatgcg tccggatgag ctgcgcggtt cggccggcag ttaccggctg
     1681 cgcgaggaac tgctggcgcg cgccaacatc gccgtcgcac cggcgcatat caacactgtc
     1741 ctgttcatcc agttgctgat ccaatgagcg acagcggcaa gcccccgtcc gacgaagaac
     1801 ttgccgccgc ctggggcgcc gaggaggcgg ccggcgacag cgccgcccag ccggcgcgcg
     1861 tgctgaacca ggcggagatc gactcgctgc tcggcttcga cgacggtccg cagggcggcg
     1921 aaaaccgcac cggcatgcag aagatcatca gcagcggcct ggtgtcctac gaacgcctgc
     1981 cgatgctgga gatcgtgttc gaccggctgg tgcgcatcat gtccaccagc ctgcgcaact
     2041 tcaccagcga caacgtcgaa gtcggcatcg acaacatcct cagcctgcgg ttcggcgatt
     2101 acctgaactc catcccgctg cccgccatgc tggcggtgtt caaggcggag gagtgggaca
     2161 atgacgggct gatggtggtg gacagcgcga tgatctactc gatcgtcgac gtgttgctgg
     2221 gcggccggcg cggcaccgcg gcgatgcgca tcgagggccg tccctacacc accatagagc
     2281 gcacgctggt ggagcggctg atcacggtgg tgctcgccga tctgtccgcc agcttcgatc
     2341 cgctgtgccc ggtgaccttc cgtttcgagc ggctggaggt gaacccgcgc tttgccacga
     2401 tcagccgcct gtccaatgcc gctgtgctgg cgcggctgcg cgtggatatg gaggaccgcg
     2461 gcggacgcat ggaactgctg ctgccctatg cgacgctgga accggtgcgc gagctgctgc
     2521 tccagcagtt catgggagag aagttcggcc gcgattcgat ctgggaaacc catctcgccg
     2581 aggagctttg gaacaccgaa gtcgaactg
//
LOCUS       DASSZH010000585         2253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_201752, whole genome shotgun sequence.
ACCESSION   DASSZH010000585 DASSZH010000000
VERSION     DASSZH010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201752"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VFN42_12680"
     CDS             <1..284
                     /locus_tag="VFN42_12680"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HET8997515.1"
                     /translation="DQAYGQCMYSRGNDVQGFPPHAQTASTLSPNDPSLVRSVQSELI
                     RLHYLAPPADGIAGPKTVSAITRFQQGAGMQPTGQASPALLARLQSTPG"
     gene            complement(417..1490)
                     /locus_tag="VFN42_12685"
     CDS             complement(417..1490)
                     /locus_tag="VFN42_12685"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8997516.1"
                     /translation="MANVGESKGEYRGGVYREDAALAAWLARRPREAALEPGLPIIDP
                     HHHFWDMPHRGHYLLPELLDDIGGGHNIVSTVFLECRAMYRKHGPREMAALGEVEFVT
                     GIAAMSESGGYGPCRVAEGIIGGGDLSIGARVRELFEAEITAAGGRLRGIRHGVAWDA
                     HESVGKYASRMVPGHLVLDPKFREGIAQLAPLGLSFESWQYHPQLPDAIDLMRAFPGT
                     TFILNHVGGILGVGIYNGHRAEILARWRQHITEMARCPNVYCKLGGLGMVSVGFDFHE
                     RDEPPSSEDLAAAWRPYIEHCIEAFGVDRCMFESNFPPDKQSCGYTELWNAFKRITAG
                     ASDAEKAALYSGTAARVYRLTAP"
     gene            complement(1520..1981)
                     /locus_tag="VFN42_12690"
     CDS             complement(1520..1981)
                     /locus_tag="VFN42_12690"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HET8997517.1"
                     /translation="MTDARLAPNLPNWMLEHANRYLSSGGTDGHMYRMAQPGRPEIVV
                     PSLLLTTTGRKSGEKFIFPLFYGEAGDSYFVIASKGGAPEHPGWYRNILANPEVEVQV
                     GTRKVKARARTATGEERARLWEKSLEFWPPYADYQKKTQREIPVVVLDPVA"
     gene            complement(2041..>2253)
                     /locus_tag="VFN42_12695"
     CDS             complement(2041..>2253)
                     /locus_tag="VFN42_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HET8997518.1"
                     /translation="PALAFFVVGILAAITVTVVGVDEADLAQGKISIASPVALALLRA
                     RKGDEVTVRTPRGAELIEVLDIRYPG"
BASE COUNT          375 a          806 c          706 g          366 t
ORIGIN      
        1 acgaccaggc ctatggccag tgcatgtatt cccgcggcaa tgacgtgcag ggcttcccgc
       61 cgcacgcgca gaccgcatcc acgctctcgc ccaacgaccc ctcgctggtg cgcagcgtgc
      121 aatcggaact gatccgcctg cactatcttg ctccgccggc cgacggcatc gctgggccca
      181 agaccgtctc cgccataacg cgtttccagc agggcgccgg tatgcagcct accggccaag
      241 cttccccggc gctgttggcg aggttgcaat cgactcccgg ctgacaagct gactggtaag
      301 cgttcggctt tgctccggaa gcgtgtgaca aacaccttcc ggagctatcc gttgaggcca
      361 actggagaac ggaagcccgc gcaacagagg ggcaaccaga tatttccccg ccgctcttag
      421 ggcgcggtca gccggtacac ccgggcggcg gtgccgctgt acagcgcggc cttctccgca
      481 tcggaggctc cggcggtgat gcgtttgaac gcgttccaca gctcggtgta gccgcaggac
      541 tgcttgtcgg gcgggaagtt gctctcgaac atgcagcgat cgacgccgaa agcctcgatg
      601 cagtgctcga tatacggccg ccacgccgcc gccagatcct ccgacgaggg cggctcgtcg
      661 cgctcgtgga aatcgaagcc gacggacacc atgcccagcc cacccagctt gcaatacacg
      721 ttggggcatc gcgccatctc ggtgatgtgc tggcgccagc gcgccaggat ctccgcccgg
      781 tgcccgttat agatcccaac gccgaggatg ccgccgacat ggttcaggat gaacgtggtg
      841 cccgggaacg cgcgcatcag gtcgatcgcg tcgggaagtt gcggatggta ctgccatgac
      901 tcgaagctca gccccagcgg cgccagttgc gcgatgcctt cgcggaattt cgggtcgagc
      961 accagatgcc ccggcaccat gcgcgacgcg tacttgccga ccgactcatg cgcatcccac
     1021 gccacgccat gccggatgcc gcgcaagcgc ccgccggcgg cggtgatctc cgcctcgaac
     1081 agctcccgca cacgcgcacc gatcgacagg tcgccgccgc cgatgatgcc ttccgccacg
     1141 cggcatggcc cgtagccgcc ggactcgctc atcgccgcga tgccggtgac gaactccacc
     1201 tcccccagcg ccgccatctc gcgcggaccg tgcttgcggt acatcgcccg gcattccagg
     1261 aacacggtgg agacgatgtt gtgcccgccg ccgatgtcgt ccagcaattc cggcagcaga
     1321 tagtggccgc gatgcggcat gtcccagaaa tggtgatgcg gatcgatgat cggcaatccg
     1381 ggttccagcg ccgcctcgcg cggacgccgg gcgagccacg cggccagcgc cgcgtcctcc
     1441 cgatagacgc cgccgcggta ttcgcccttc gactcgccga cattcgccat gacggttccc
     1501 ccctttgccg cgcgcgcctt caggcgaccg ggtccagcac caccaccgga atctcgcgct
     1561 gcgtcttctt ctggtaatcc gcgtaaggcg gccagaactc cagcgacttc tcccacagcc
     1621 gagcgcgctc ctcgcccgtc gcggtgcgcg cccgcgcctt caccttgcgc gtgccgacct
     1681 gcacctcgac ctccggattg gccaggatgt tgcgatacca gccgggatgc tccggcgcgc
     1741 cgcccttgga cgcgatgacg aagtagctgt caccggcttc gccatagaac agcgggaaga
     1801 tgaacttctc gccggacttg cggccggtgg tcgtcagcag cagtgacggc acgacgatct
     1861 cgggccggcc cggctgggcc atgcggtaca tgtgcccgtc ggtgccgccg ctggacaggt
     1921 agcggttggc gtgctccagc atccagttgg ggaggttggg cgcaagtctg gcgtcggtca
     1981 tgatcgcggc ttcccttcaa tgccggacgc atcctcggcc agtccggagc gccgggcaaa
     2041 tcagccggga tagcggatgt ccagcacctc gatcagttcc gccccgcgcg gcgtgcgcac
     2101 ggtgacttcg tcccccttgc gcgcgcgcag caaggccagc gccaccggcg aggcgatgct
     2161 gatcttgccc tgcgccagat ccgcctcatc cacgccgaca acggtgacgg tgatcgcagc
     2221 gagaatgccg actacaaaga aggcaagcgc cgg
//
LOCUS       DASSZH010000586         6183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1411289, whole genome shotgun sequence.
ACCESSION   DASSZH010000586 DASSZH010000000
VERSION     DASSZH010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6183
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411289"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VFN42_12700"
     CDS             <1..456
                     /locus_tag="VFN42_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997519.1"
                     /translation="PAIAAMHEATYAFAHPAAMQGQPAEMALAVACLDAMAGQFSTVG
                     RWLDMSRLAQLEMLEARAHVRAMLGIAPEVPSQAVIDALVAASHALDRGNRAAAERSL
                     TGPGFSMAPTQMLALLAHFPPVPVANRATMRASRYLYPGGGGAFEWNPF"
     gene            621..797
                     /locus_tag="VFN42_12705"
     CDS             621..797
                     /locus_tag="VFN42_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997520.1"
                     /translation="MQAAPSPDSRSGFSAIPGFGGQSTQNSGGNSLAASPNTVNDIED
                     KVLVGARQRRVGMQ"
     gene            complement(1062..2306)
                     /locus_tag="VFN42_12710"
     CDS             complement(1062..2306)
                     /locus_tag="VFN42_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HET8997521.1"
                     /translation="MNKYLLGALISIGTIVGASGAANAQAKHAPAPGTIAVHLNGLVD
                     WQTNVIGSSANDYNGYKLNSLGMGGYFRLYAGFDATTKGGLQYGADSELRLTYTPSAV
                     GEPESAPNAESMNLRRAWIYIGTPQVGFVRVGQTNSPWLLLTTGEYYNWGDHNGWNSD
                     GGIAAAVPSATHPSALFNASGVLYTTNKIVYISPNFAGFQAGISYEPNSNSFKNGEQC
                     TVAAAGCNTVESGPVGVVSTGMRRNTVVGVVKYANKFGGFGVDVDAGYLTATPALSGG
                     AGLRDLSVGSIGGQITFGGFLLGGNIKFGQVNNGYTFLRPGQRDLLFYNLTGEYSMGP
                     ARFAVNFFNNQGAGSVDRTQSNYGIGVGANYDLAKGVDLYAMYLYGHRHEAGYDFLAG
                     GPGVAHNSVDANAFTVGTVVTW"
     gene            complement(2784..3656)
                     /locus_tag="VFN42_12715"
     CDS             complement(2784..3656)
                     /locus_tag="VFN42_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006557794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET8997522.1"
                     /translation="MSARPQERRLGRGLASLLGDLSPGRVNPAGGNVRHLSLDQLSVN
                     PYQPRGHIDATALEELAESIRAYGVVQPLLARPDPRDPDRYTLIAGERRWRAAALAGL
                     QEVPVLVQELSDVDAAAIALVENLQREDLNPMEEAEGYNRLLDEFAMTQETLAKAVGK
                     SRTHVTNALRLLNLPESVQAEVRQGHLSFGHARALLSHPNPEALLPTVLGKQLNVRQT
                     EALAAKPPRKPARPAAPAASGRFADTASLERRLTEALGYKVEVAIEPGGGGSVRIRFD
                     DLHQLSEITERLTG"
     gene            complement(3653..4486)
                     /locus_tag="VFN42_12720"
     CDS             complement(3653..4486)
                     /locus_tag="VFN42_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HET8997523.1"
                     /translation="MPSGTEHSSTPRGVRIIAVANQKGGVGKTTTAVNLATALAAKGE
                     PVLLIDLDPQGNASMGFGFARDAKTRGTYHLLSGEASLADASHDTFVPNLTLIPSDID
                     LAGAEVELVGEQRREYRLRDALAALRGGGTRGFVVIDCPPSLGLLTLNALVAAHSVMV
                     PLQCEFYALSGIGALMHTVDMIRRRLNPNLRVEGIVLTMHDRRQSLSDLVAEDARGVF
                     GDLVYSTVIPRNVRVSEAPSHGKPVLLYDFRSPGSQAYIRLAAEMLERQRGIVDGGAA
                     R"
     gene            complement(4722..5711)
                     /locus_tag="VFN42_12725"
     CDS             complement(4722..5711)
                     /locus_tag="VFN42_12725"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET8997524.1"
                     /translation="MPNALAHRKLGSGGLSVSALGLGGMSLSGVYGPADDAQSEDLIR
                     YAIDQGVDHIDSSDMYGWGHNEELIGRAIKGRRDWVVLATKFGQTQNPGGPNGVNGRP
                     EYVQQACEASLKRLGEEVIDLYYQHRVDPGVPIEDTVGAMAKLVQQGKVRFLGLSEAA
                     PDTIRRAHAVHPIAAVQTEYSLLYRAEAEETRATTTELGIGYVAYAPLGRGFLTGAIK
                     TLDDVDGRRAQHPRFQAENFAHNRDLVARVEAIAAEKGCTPSQLALAWVLAQGEDVTA
                     IVGTRRKERLMENLGALNVQLNPADVERISSVVPRGAAAGTRYPAGGMRGVFL"
     gene            complement(5909..6109)
                     /locus_tag="VFN42_12730"
     CDS             complement(5909..6109)
                     /locus_tag="VFN42_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997525.1"
                     /translation="MIDRGEILWLGVASSLSGGLIGGLMLGIGVVVVLSGAAWGWLVM
                     LAGVPASALPGWIMAYRLGRQL"
BASE COUNT          975 a         2082 c         2151 g          975 t
ORIGIN      
        1 ccggcgattg cggcgatgca tgaggccacc tatgccttcg cccatccggc ggcgatgcaa
       61 ggacagccgg cggagatggc gcttgccgtc gcttgtctcg atgcgatggc cggccagttc
      121 tccaccgtgg gccgctggct cgacatgagc cggctggcgc agcttgagat gttggaggcg
      181 cgggcgcacg tgcgggcgat gcttgggatt gcgcccgagg tgccgtcgca ggcggtcatt
      241 gatgcgctgg tcgcggcctc gcacgcgctc gatcggggta accgggcggc ggccgagcgg
      301 tcgctgaccg gccccggttt ctcgatggcg cccacccaga tgctggcgct gctggcccat
      361 ttcccgccgg tgccggtagc caatcgggca acgatgcggg caagccgcta cctgtatccg
      421 gggggtggtg gcgccttcga gtggaacccg ttctaggaca gcgcggccgt cgcgcgaatc
      481 gcccatgacc gatgcggcga cgcggccagc cgaggcgttc gcggtccgcg acggatcgcc
      541 tgagtagcgg cgccgaaaag cgcccaaggt gccgttgcgc cagcctggcc ggctcctcgc
      601 cgcgtctgca atccggcgag atgcaggcgg cgccatcgcc ggatagccgc agcggcttca
      661 gtgcgatccc cggctttggc ggccagagca cgcagaacag cggcggcaat tcgctggcgg
      721 ccagcccaaa cacggtgaac gatatcgagg acaaggtgct cgtcggcgcc cggcagcggc
      781 gcgtggggat gcagtgaaca accagccggg caggtctggc ggccggcggt gcctggggga
      841 tcgggcgcat tcgccggtgc ccgatccgta gcacctgcat caccggcgac tgccattcgc
      901 caatccaggc tggagcgcgg cgctctgccc cggcggaacg gccatcactg ctgctcgcca
      961 ggcgcggtga ccgggccggc ggaccacggg cgacggccaa aaagaaaggg tggcggattg
     1021 ctccgccacc cccgtcaagc cgcagtggtt ggtgcgtcga attaccaggt gacgaccgtg
     1081 cccaccgtga acgcgttggc atcgacgctg ttatgcgcga cgccggggcc gccggcgagg
     1141 aagtcgtaac cggcctcgtg acgatgaccg tagagataca tcgcgtagag gtcgacgccc
     1201 ttcgccaggt cgtagttcgc gccgacgccg ataccgtagt tcgactgcgt gcggtcgacg
     1261 ctgccggcgc cctggttgtt gaagaagttc acagcaaagc gggccgggcc catgctgtac
     1321 tcgccggtga ggttgtagaa cagcaggtca cgctggccag gacgcaggaa cgtatagccg
     1381 ttgttgacct gaccgaactt gatgttgccg cccagcagga agccaccgaa ggtgatctgg
     1441 ccgccgatcg aacccacgct cagatcgcgc aggccggctc cgccggacag ggcgggcgtc
     1501 gccgtcaggt agccggcatc gacatcgacg ccgaagccgc cgaacttgtt ggcatacttg
     1561 accacgccga cgaccgtgtt ccggcgcatg ccggtggaaa ccacgcccac cggaccgctc
     1621 tccacggtgt tgcagcccgc ggccgcgacg gtgcactgct caccgttctt gaacgagttg
     1681 gagttcggct cgtagctgat gccggcctgg aagcctgcga agttcggcga gatgtagacg
     1741 atcttgttgg tggtgtacag gacaccgctc gcgttgaaca aggccgacgg gtgcgtcgcc
     1801 gacggcaccg ccgcggcaat gccaccatcc gagttccagc cgttatggtc gccccagttg
     1861 tagtactcac cggtggtgag caggagccac gggctgttcg tctggccgac ccggacgaaa
     1921 ccgacttgcg gcgtgccgat gtagatccac gcgcggcgca ggttcatgga ttcggcgttc
     1981 ggggcgcttt ccggctcacc cacggcgctg ggcgtatagg tcaggcggag ctcggagtcg
     2041 gcgccgtatt gcaggccgcc cttggtcgtt gcgtcaaatc ccgcatacag gcggaagtag
     2101 ccgcccatgc ccagcgagtt gagcttgtag ccgttgtaat cgttggccga cgaaccgatc
     2161 acgttcgtct gccagtcgac caggccgttc aagtgcaccg caatggtgcc aggggccggg
     2221 gcgtgcttcg cctgggcgtt agcagcgccg ctcgcaccca cgatcgtacc gatcgatatg
     2281 agtgctccca gcaggtactt attcatcagt ttcccatcct tcctgttgcc tcggtagcac
     2341 catcggatcc actccattgc caacacggca gcaacttcgg atccgcgcgg ttcaatggcg
     2401 ccggaccgtt gcagtatgat catcggcaat gcgtcctcgc aacggctgtt ggcaccctca
     2461 cggagctatc gccaagcgcc gtgaccaaaa tgccacaact gctcagcggc caggccacac
     2521 ctttggaatc acgccgcgtg ggactggcgg ccgcactggc aaagtgttgc cccggatgca
     2581 ccgtacacga ccaagtcaac cggccggtgg cggaacgttg ctcccgcaac aggcgcaatg
     2641 cggtcgtccg tccggcaaag tcattcggca agcggcagcg cggcaggatg cgtcccgccg
     2701 ccgggaagcc tagttgcgac tttcgtgttg cctggcgcgc tcgccggcaa ggccgtcgcg
     2761 gctatcgccc gctgaccggc atctcagccc gtcagccgct cggtgatttc gctcaactgg
     2821 tgcaggtcgt cgaaccggat gcgcaccgaa ccgccgccgc cgggctcgat cgccacctcc
     2881 accttgtacc ccagcgcctc ggtcagccgc cgctccaggc tggcggtatc ggcgaagcgg
     2941 ccgcttgccg cgggtgcggc cgggcgggcg ggcttgcgcg gcggctttgc ggccagcgcc
     3001 tcggtctgcc gcacgttgag ctgcttgccg agcacggtgg gcagcagcgc ttcagggttg
     3061 gggtggctga gcagcgcgcg ggcatgcccg aagctgaggt ggccctgccg cacctccgcc
     3121 tgcaccgact cgggcaggtt cagcagccgc aaggcgttgg taacatgggt gcgggacttg
     3181 ccgaccgcct tggccagggt ctcctgggtc atggcgaact cgtcgagcag gcggttgtag
     3241 ccctccgcct cctccatcgg gttcaggtcc tcacgctgca ggttctccac cagggcgatg
     3301 gccgcggcat cgacgtcgga cagttcctgc accagtaccg gcacttcctg caggccggcc
     3361 agcgcggcgg cgcgccagcg gcgttcgccg gcgatcagcg tgtaacggtc gggatcccgc
     3421 ggatccggcc gcgccagcag cggctgcacc acgccgtacg cccggatcga ttcggccaac
     3481 tcctccagag ccgtcgcgtc gatatggccg cgcggctgat aggggttcac cgaaagctgg
     3541 tccagcgaca ggtggcgcac attgccgccg gcgggattga cgcgcccggg ggacaggtcc
     3601 cccagcagcg acgccaggcc gcggccgagc cggcgttcct gcggccgggc gctcatcggg
     3661 cggcgccgcc gtccacgatg ccgcgctgcc gctccagcat ctccgcggca aggcggatat
     3721 aggcctgcga tccgggagag cggaaatcgt acagcagcac cggcttgccg tggctcggcg
     3781 cctcggagac ccgcacattg cgggggatga ccgtgctgta caccaggtcg ccgaacacgc
     3841 cacgcgcgtc ctcggccacc aggtcggaca ggctctgccg gcggtcgtgc atggtcaaca
     3901 cgatgccctc gacacgaaga ttcgggttca agcggcgccg gatcatgtcc accgtatgca
     3961 tcagtgcgcc gattccggac aacgcataga attcgcactg cagcggcacc atcaccgaat
     4021 gtgcggcaac cagagcgttc agcgtcagca ggcccaggct gggggggcag tcgatcacca
     4081 cgaagccacg ggttcccccg ccgcgcaggg cggcaagcgc gtcacgcagc cggtattcgc
     4141 ggcgctgctc ccccaccagt tccacctcgg cgccggcaag gtcgatgtcg gacgggatca
     4201 atgtcaggtt ggggacaaag gtgtcgtggc tggcatcggc cagcgaggct tcgccggaca
     4261 gcagatgata ggtgccgcgc gtcttggcgt cgcgggcaaa gccgaatccc atcgaggcgt
     4321 tgccctgcgg gtccaggtcg atcagcagca ccggctcgcc cttggcggcc agcgccgtgg
     4381 cgaggttcac cgcggtcgtg gttttgccga ccccgccctt ctgattggcg acggcaatga
     4441 tgcggacgcc cctgggcgtg gatgaatgtt cggtcccgct tggcaacggc atgtccggag
     4501 aggatgaggc cccagtgtgg cagggccgaa aacgatatgc caccgccttg ccggctgcgc
     4561 gtgcaatggg cgggggcggc cggcatacac cggctggcgg acgggtaaaa gcggaggcgt
     4621 gccagctggc tgccgggccg ccggccgccg aatcgcgcgg cgccgcatcg tgggtggcga
     4681 tggtgcgcgg cgcgactcga tctgtgccgg gttggcggcg gctacaggaa cacgccccgc
     4741 atcccgccgg cgggatagcg cgtacccgcc gcggcgccgc gcggcaccac ggacgagatc
     4801 cgctccacat ccgccgggtt gagctgcacg ttcagtgcgc cgaggttctc catcagccgc
     4861 tccttccggc gcgtgccgac gatcgccgtg acgtcctcac cctgcgccag aacccaggcc
     4921 agcgcgagct gcgagggggt gcagcccttc tccgccgcga tcgcctccac cctggccacc
     4981 aggtcgcgat tgtgggcgaa gttctccgcc tggaagcgcg gatgctgcgc gcggcggccg
     5041 tccacgtcgt ccagcgtctt gattgcgccg gtcaggaagc cgcggccgag cggggcgtag
     5101 gcgacgtagc cgatgcccag ttcggtggtg gtcgcacggg tttcctcggc ctcggcgcgg
     5161 tagagcaggg agtattcggt ctgcaccgcg gcgatcggat gcaccgcatg ggcgcggcgg
     5221 atggtgtccg gcgcggcttc cgacaggccc aggaagcgta ccttgccctg ctgcaccagc
     5281 ttggccatgg cgccgacggt gtcctcgatc ggcacgccgg gatcgacgcg gtgctggtag
     5341 tacaggtcga tcacctcctc gccgagccgc ttgagggagg cttcgcaggc ctgctggacg
     5401 tattcggggc gaccgttgac gccgttggga ccgccgggat tctgggtctg gccgaatttg
     5461 gtggccagca cgacccagtc ccgccggccc ttgatcgcgc ggccgatcag ctcctcgttg
     5521 tggccccagc catacatatc ggaggagtcg atgtggtcca cgccctggtc gatcgcgtag
     5581 cggatcaggt cttccgattg ggcgtcgtcc gccgggccat agaccccgga cagcgacatg
     5641 ccgcccaggc caagggcgga caccgacagt ccgccggagc cgagtttccg gtgtgcgaga
     5701 gcgttgggca tgcaagcctc cccgttggat tgggcggcac cctaggggcg ggcggaaggc
     5761 ctgtcacggg ggcggcgcgc cggcatcgga tgaagttgga gtcctctcca gccgctgcgc
     5821 ctcgccgcgg aagccgtcat cgggacctgc ttgccgccgc tggggcaatg agggccgggc
     5881 ggcaaacaac ccggagcagc aaccccggtt atagttggcg cccgagccgg taggccatga
     5941 tccagccggg cagcgcggag gccggcacac ccgccagcat caccagccag ccccaggcag
     6001 cgccgctgag caccacgacc acgccgatgc ccagcatcag gccgccgatc aggccgccgg
     6061 acaggctgga cgcgacgcca agccacagga tttcgccacg atcgatcatg ccagcctgct
     6121 acgggcaatc gggaccgtga cgcaagcggc acggcggaat tcctgggcca caggggcaga
     6181 att
//
LOCUS       DASSZH010000587         2397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_627964, whole genome shotgun sequence.
ACCESSION   DASSZH010000587 DASSZH010000000
VERSION     DASSZH010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627964"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..1066)
                     /locus_tag="VFN42_12735"
     CDS             complement(14..1066)
                     /locus_tag="VFN42_12735"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014105853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HET8997526.1"
                     /translation="MSFATVAPAESLLRWYDQHRRRLPWRSEPGRQPDPYRVWLSEIM
                     LQQTTVAAVIPYFNKFVSRFPSVAALAAAPLDAVLAAWAGLGYYARARNLHACAQAVA
                     AVGAFPRDLEGLRALPGIGEYTAAAVGAIAFGIPVVPVDGNVERVVARLFAVAQPLPG
                     AKPDLRRLAAQIGDDPAARARPGDYAQALFDLGATTCTPTTPACALCPWMRGCSARQA
                     GIAAELPRKTPKPERPLRRGVHFWLVDETGHVLLRRRAPDGLLGGMTELPGTPWRSAA
                     WTRAEALAHAPMTADWRSAGEIRHGFTHFALTIDLLAARVPRIEADGFLRPVTELHDE
                     ALPSVMRKCVRIGSTA"
     gene            1053..1556
                     /locus_tag="VFN42_12740"
     CDS             1053..1556
                     /locus_tag="VFN42_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010513560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HET8997527.1"
                     /translation="MAKDTRPDIASQPRHVYGPRPIGALVPRLTRPAFRRISPAAAQV
                     MADWASIVGPALAAVTTPHRLSAGQLTIACSGPIAMELQHLATELIARINTHLGTSTV
                     KALRFLQTLPPAPASPQAQASPPTVADRRRAEAAVSDLPQGDLRSALASLGAAVLSAK
                     GHTKAST"
     gene            1599..2198
                     /locus_tag="VFN42_12745"
     CDS             1599..2198
                     /locus_tag="VFN42_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HET8997528.1"
                     /translation="MLLSRRVLLSVVAASAALGSAARAAEIDPESPRPVGNPDAKVTV
                     EEWFSLTCTHCADFAKETFPEIKAKLIDTGKVRWVFRDFPLDQVALLAAQVAHYLPPD
                     RYEPFVLALFASQDRWAFAQGVNTTDELWKMAALAGMNKADFDKAIADTKLRNWILQE
                     QSVAQKKFGIDSTPSFVIGGKVYPGEMPYDAFVKLLPGT"
     gene            2214..>2397
                     /locus_tag="VFN42_12750"
     CDS             2214..>2397
                     /locus_tag="VFN42_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8997529.1"
                     /translation="MPVSFRRLRIAGFKSFAEPVSVEIMPGLTGIVGPNGCGKSNVVE
                     ALRWAMGENNARTMRGA"
BASE COUNT          379 a          861 c          810 g          347 t
ORIGIN      
        1 gtcggaaggg gggctacgcc gtgctgccga tccgcacgca tttgcgcatc accgacggca
       61 gtgcctcgtc gtgcagttcg gtgaccgggc gcaggaatcc gtccgcctcg atccgcggca
      121 cgcgcgcggc cagcaggtca atggtcagcg cgaagtgggt gaagccgtgc cggatctcgc
      181 ccgcgctacg ccagtccgcc gtcatcggcg cgtgcgccag cgcttccgcc cgggtccagg
      241 cggcagagcg ccagggcgtg cccggcagtt ccgtcatgcc gccgagcagc ccgtccggcg
      301 cccggcggcg cagcagcacg tggccggttt cgtccaccag ccagaaatgc acgccgcgcc
      361 gcagcgggcg ctcgggtttc ggcgtcttgc gcggcagctc cgccgcgatg cctgcctgac
      421 gagccgaaca gccgcgcatc caggggcaca gggcgcatgc cggcgtggtg ggggtgcagg
      481 tcgtcgcccc aaggtcgaac agcgcctgcg cgtagtcgcc gggacgcgcc ctcgcggccg
      541 ggtcgtcgcc gatctgcgcc gccagccgtc gcaggtccgg cttcgctccc ggcagcggct
      601 gcgccaccgc aaacaggcgc gccacgaccc gctcgacatt gccgtccacc gggaccaccg
      661 ggatgccgaa cgcgatcgcc ccgaccgccg ccgcggtgta ctcgccgatc ccgggcagcg
      721 cgcgcaggcc ttccaggtcg cgtggaaatg cgccgaccgc cgccaccgcc tgggcgcagg
      781 cgtgcaggtt gcgggcgcgc gcgtagtagc caagcccggc ccaggcagcc agcacggcgt
      841 ccagcggcgc cgccgccaaa gcagccacgc tggggaagcg gcttacgaac ttgttgaaat
      901 acggtatgac ggcggcaacc gtcgtctgct gtagcatgat ctcgctcagc cagacgcggt
      961 acggatccgg ctgccgcccc ggctcgctcc gccacggcag acggcgccgg tgctggtcgt
     1021 accagcgaag cagcgactcg gcaggagcga cggtggcaaa ggacacgaga cccgatatcg
     1081 caagccagcc acggcacgtc tacggcccgc gcccgatcgg cgcgctggtg ccacgcttga
     1141 cgcgccccgc gttccggcgg atcagcccgg cagcggcgca ggtgatggcg gactgggcct
     1201 cgatcgtcgg gccggcgctg gccgcggtca ccacgccgca ccggctgtcg gccggccagc
     1261 tcaccatcgc ctgctccgga ccgatcgcca tggaattgca gcaccttgcc accgaactga
     1321 tcgcccgcat caacacccac ctgggcacga gcacggtgaa ggctctgcgt tttctgcaga
     1381 cgctgccgcc cgcgccggct tccccccagg cccaggcttc cccacccacc gtcgccgatc
     1441 gccgccgcgc cgaagcggcg gtgtccgacc tgccccaagg cgacctgcgc tccgccctgg
     1501 cctcactggg cgcggcggtg ctgagcgcca aggggcacac gaaagcctcg acttgaacgt
     1561 cctgccggca gtagaacata ccagatatag aggttccgat gcttctctcc cgccgagtcc
     1621 tactatccgt tgttgccgct tcggccgcgc tgggcagcgc ggcgcgcgct gcggagatcg
     1681 atcccgaatc gccgcgcccg gtcggtaacc cggacgccaa ggtcacggtg gaggagtggt
     1741 tctccctcac ctgcacccac tgcgccgatt tcgccaagga gaccttcccg gaaatcaagg
     1801 ccaaactgat cgataccggc aaggtgcgtt gggtgttccg cgacttcccg ctggaccagg
     1861 tggcgctgct ggcggcccag gtggcgcatt acctgccgcc ggaccgctat gagccgttcg
     1921 tcctggcgct gttcgccagc caggaccgct gggcctttgc ccagggcgtc aacaccacgg
     1981 acgagctgtg gaagatggcg gcgctggccg gcatgaacaa agccgatttc gacaaggcga
     2041 ttgccgatac gaagctgcgt aactggatct tgcaggagca gagcgtggcg cagaagaagt
     2101 tcggcatcga ctcgactccg agctttgtga tcggtggcaa ggtctacccg ggtgaaatgc
     2161 cttacgacgc cttcgtaaag ctgctccccg ggacctgacc gaaggagaag cgtttgcccg
     2221 tcagtttccg ccgcctccgc attgccgggt tcaagagttt cgccgaaccg gtcagcgtcg
     2281 agatcatgcc gggactgacg ggcatcgtcg ggccgaacgg ttgcggcaag tccaacgtgg
     2341 tggaagcgct gcgctgggcg atgggcgaga acaacgcccg caccatgcgc ggcgccg
//
LOCUS       DASSZH010000588         2209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1070813, whole genome shotgun sequence.
ACCESSION   DASSZH010000588 DASSZH010000000
VERSION     DASSZH010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070813"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VFN42_12755"
     CDS             complement(<1..617)
                     /locus_tag="VFN42_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET8997530.1"
                     /translation="MNKPVPPQIGAALLGGRISPTAAFPPGLDPRSAAVLREIVEQYV
                     ETGEPVGSRTLSRRLPMNLSPATIRNVMADLTDSGLLFAPHTSAGRLPTEQGLRLFVD
                     GLLNFGELGEEERETIAAVLAAAGRSLEDTLAEASLMLSGLSSAAGLVLAPKSEGPLR
                     HIEFVPLGPGRALVVLVNAEGQVENRVMEIPPGLPPSALQQASNYL"
     gene            complement(785..1531)
                     /locus_tag="VFN42_12760"
     CDS             complement(785..1531)
                     /locus_tag="VFN42_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997531.1"
                     /translation="MTLAAFQAALGHLVRSGESGGWDQPALSANERVLLHDVAESAGL
                     RLTAAIQRSWCEGRAAKAARLTLAMLPPAERRGLVEAWVAAGGGTNSFHATETERFCA
                     FIAERLADPSHALTVCRLEQATMRAALASETYVPVRCDAEPDPDCALVAGRGAGLVPF
                     FASPAEVLGALRGGVWPACGPVQAWLLFAPGLDGLARPASEAETLLWFRLTRAVAAGE
                     TVKTLVAAGFEPALIRAFLGIGAAEPASGR"
     gene            complement(1528..>2209)
                     /locus_tag="VFN42_12765"
     CDS             complement(1528..>2209)
                     /locus_tag="VFN42_12765"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HET8997532.1"
                     /translation="AHGMNDAYLAMLDRFQTAWPFAWHSEHLSFQTFRDSGGAVRDTG
                     VPLPLPPTEEAAQLVAQRCVAIRRRYGLPFLLENAVHYLPRLPTDSGIGDDIGLMNHI
                     VRAGGCGQLLDLHNIYCNAVNFGFDAFAAIDRMYLDQVGEIHIAGGSWSGGFRVDAHD
                     NVVPAAVWDLLDYALPRCRNAAGVVFEILDEHVARVGADAVAADLARARAAWRSARLQ
                     AAPAEPAT"
BASE COUNT          384 a          762 c          762 g          301 t
ORIGIN      
        1 aggtagttgc tggcctgctg cagggcggag ggcggcaggc cgggcgggat ttccatcacc
       61 cggttctcca cctggccctc ggcgttcacc agcaccacca gggcgcggcc agggccgagc
      121 gggacgaatt cgatgtggcg gagcgggccc tccgacttgg gagccagcac caggccggcg
      181 gcggaggaca ggccggacag catcaggctg gcctcggcca gggtgtcctc caggctgcgg
      241 ccggccgcgg ccagcacggc ggcgatggtt tcccgctcct cctcacccag ctcaccgaaa
      301 ttcagcaggc cgtcgacgaa cagccgcagc ccctgctcgg tgggcagccg cccggccgag
      361 gtatgtggcg cgaacagcag gcccgagtcg gtcaggtcgg ccatgacgtt gcggatggtg
      421 gcgggcgaga gattcatcgg cagccggcga gacagggtgc ggctgccgac cggctcgccg
      481 gtctccacgt attgttcgac gatctcccgc agcacggcgg cggaacgcgg gtccaggccg
      541 gggggaaacg cggcggtggg ggatatgcgc ccgcccagaa gggcggcgcc gatttggggc
      601 ggaacgggtt tgttcatgct atgggctccc gactcacaga acgtaggcag caggagtccg
      661 tgggtcaatc gtgcggttga tcgggcgcag cgacataagc gcattcgctg cctcgggcgg
      721 cgtcaacgcc agcgcgccgg catcactgtt tcatgtccgg ctcggccgaa tggaacccgc
      781 cgcatcaccg gcctgaagca ggctccgcgg cgccaatacc caggaaggcg cggatcaacg
      841 cgggctcgaa gccagccgcc acgagcgtct tcaccgtttc gcccgctgcg acggcccggg
      901 tgaggcgaaa ccagagcagg gtttccgcct cgctcgccgg gcgggccagt ccgtcgagcc
      961 cgggggcgaa cagcagccag gcttgcaccg gaccgcaagc tggccagacg ccgccgcgca
     1021 gggcgccaag cacctcggcg ggagacgcga agaagggcac cagcccggca ccacggccag
     1081 ccaccagcgc acaatccgga tcaggctctg catcgcagcg gacaggaaca taggtttccg
     1141 acgcaagcgc ggcacgcatc gtggcctgct ccagtcggca caccgtcagc gcatgcgatg
     1201 gatcggccag gcgctcggcg atgaacgcgc agaagcgctc ggtctcggtc gcatgaaacg
     1261 aattcgtgcc gccacctgcc gcaacccatg cctcgaccag tccgcgccgt tctgccggcg
     1321 ggagcatcgc caaggtcagg cgggcggcct ttgcggcgcg cccctcacac caggagcgct
     1381 ggatcgccgc ggtcagccgc agaccggcgc tctccgcgac atcgtgcaac agcacgcgtt
     1441 cattggcgga cagcgcgggc tggtcccagc cgccgctttc gccggatcgc accagatgcc
     1501 cgagcgcggc ctgaaacgcc gccagtgtca tgtcgcgggc tcagccggcg cagcttgcaa
     1561 gcgggcgctg cgccacgccg cgcgtgcccg cgcaagatcc gccgcgaccg catccgcacc
     1621 gacgcgtgcc acgtgctcgt ccaggatttc gaacaccacg cccgcggcat tgcggcagcg
     1681 cggcagcgcg tagtccagca agtcccacac cgctgccggc acgacattgt catgcgcgtc
     1741 cacgcggaat ccgccgctcc aggatccgcc ggcgatatgg atttcgccaa cctggtccag
     1801 gtacatccga tcgatcgccg cgaaggcatc gaagccgaaa ttcactgcat tgcagtagat
     1861 gttgtgcaga tcgagcagct ggccacagcc gccggcgcga acgatgtggt tcatcagccc
     1921 gatatcgtcg ccgatgccgg aatcggtcgg caggcgtggg agataatgca cggcattttc
     1981 caacaggaat gggaggccgt agcgccggcg gatcgccacg cagcgctgcg cgacgagctg
     2041 cgcggcttcc tcggtgggcg gcaacggcag cggcaccccg gtatcgcgca ccgcgccgcc
     2101 actatcgcgg aaggtctgga aactcagatg ctcactgtgc caggcgaaag gccaagccgt
     2161 ctggaagcgg tcgagcatcg cgagataggc atcgttcata ccgtgcgcc
//
LOCUS       DASSZH010000589         2489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1275302, whole genome shotgun sequence.
ACCESSION   DASSZH010000589 DASSZH010000000
VERSION     DASSZH010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275302"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VFN42_12770"
     CDS             <1..410
                     /locus_tag="VFN42_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8997533.1"
                     /translation="SRSSLGLILQATGQDRIEAAALGFNVTKHKVAAFCVSALFSGLA
                     GAMLTFYEGIASVDTVVSLAITIQIIIAVVFGGRRTILGAILGSIFLILFDELLRPIG
                     QLNTFVVSGIALAVIIFFPDGALGWALRPRERS"
     gene            410..1882
                     /locus_tag="VFN42_12775"
     CDS             410..1882
                     /locus_tag="VFN42_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8997534.1"
                     /translation="MPDTTPLLEVSGLTKRFGGLVAVREIGFHIRPGEILGLIGPNGS
                     GKSTVMKLVMGIERPNAGVVKLSGTDIAGWPTHRIARQGIGIVFQHSRPLHRQTVLEN
                     IKLALLPDSLFRLIAAPHIEERAREIAARVGLGEVLDRRPATLPFADLRRMEMAKALA
                     RDPQVVLVDEPFAGLTAGEVADFSELIDSFRRDGRAVLLVDHNVKSVAALVDRVLAMY
                     LGQRIAEGTAEEVMADETVRRVYLGGKIESHPRKNLDVAGREALLEVRGIDVLYGKAQ
                     ALQQVSIHVNEGEFVSIVGLNGAGKTTLFNAISGLVPYSGDIHWAGGSLRGRDPWRIA
                     REGIVQCPETRELFGDLSVRENLDLGGQHLQRAEQHEQLEWLLGLFPILRERQRQPAA
                     TLSGGEQQMLAIARALMMKPKLLILDEPTLGLAPVILEQLSKALALLRETTPITVLLG
                     EQNVTFALPHADRVYVLEHARITWEGDPGRFAEEMGTGYL"
     gene            complement(1900..>2489)
                     /locus_tag="VFN42_12780"
     CDS             complement(1900..>2489)
                     /locus_tag="VFN42_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HET8997535.1"
                     /translation="NPTQVNRLAEARPIDWFERRLIGVVPLRYRGALRRVYPGFVQLT
                     AFMSMNLDRHVDAHVGQLRALVGGEDDRVEAHRRFYGEYRAVMDLPAEFFLETVERVF
                     QRHELPQGRLHWRGERVRPDAIEGTALLTVEGERDDICAIGQTRAAHDLCTAIPAERR
                     THYQQARAGHYGVFSGRRWEREIYPVLRQTIRAAD"
BASE COUNT          415 a          848 c          804 g          422 t
ORIGIN      
        1 tgtcgcgctc ctcgcttggc ctcatcctgc aggccaccgg ccaggaccgt atcgaggccg
       61 cggcgcttgg cttcaacgtc acgaagcaca aggttgcggc gttctgcgtc agcgcactgt
      121 tttccggcct cgccggcgcc atgctgacgt tctacgaagg catcgcctcg gtcgataccg
      181 tggtcagcct ggcgatcacc atccagatca tcatcgccgt ggtgttcggc ggacgccgca
      241 ccatcctcgg tgccatcctg ggttccatct tcctgatcct gttcgacgaa ttgctgcgcc
      301 cgatcggcca gctcaatacc ttcgtggtct ccggcatcgc gctggcggtg atcatcttct
      361 tccctgacgg cgccttgggc tgggcactgc gcccgcggga gcggagttga tgccggacac
      421 cacaccgctg ttggaagttt ccggcctgac caagcgcttc ggcggcctgg tcgcggtgcg
      481 tgagatcggg ttccatatcc ggcccggcga gatcctcggc ctgatcggtc cgaacggctc
      541 cggcaaatcc accgtgatga agctggtgat gggcatcgaa cgccccaacg cgggcgtggt
      601 gaaactgtcc ggcaccgaca ttgctggctg gcccacccat cgcattgccc ggcagggcat
      661 cggcatcgtg ttccagcatt cacgcccgct gcaccggcag accgtgctgg agaacatcaa
      721 gctggcgttg ctgccggatt cgctgttccg cctgatcgcc gcaccgcata tcgaggagcg
      781 tgcgcgcgag atcgccgcac gcgtcgggct gggcgaggta ctggaccgcc ggccggccac
      841 cctgccgttt gccgatctgc gccgcatgga gatggcaaag gcgctggcac gcgatccgca
      901 agttgtgctg gtggatgagc ccttcgccgg cctgaccgcc ggcgaggtcg ccgacttctc
      961 ggaactgatc gacagcttcc gccgcgacgg cagggccgtg ctgctggtgg atcacaacgt
     1021 gaaaagcgtg gccgccctgg tggaccgggt gctggctatg tacctgggcc agcgcatcgc
     1081 cgaaggtacc gccgaggaag tgatggcgga tgaaacggtc cggcgcgtct atctcggcgg
     1141 caagatcgaa tcgcacccgc gcaagaacct ggacgtggcc gggcgcgagg cgttgctgga
     1201 agtacgcggc atcgacgtgc tctacggcaa ggcgcaggcg ctgcagcagg tctccatcca
     1261 cgtcaacgaa ggcgagttcg tttcgatcgt cgggctgaac ggcgccggca agaccacgct
     1321 gttcaacgcg atttccgggc tggtgcccta ttccggtgac attcactggg ccggcggcag
     1381 cctgcggggg cgcgatccct ggcgcattgc acgcgaaggc atcgtgcagt gcccggaaac
     1441 ccgcgaactg ttcggcgatc tgagcgtgcg ggaaaatctc gaccttggcg ggcagcacct
     1501 gcagcgcgcg gaacagcatg aacaactgga atggctgctc ggcctgttcc cgatcctgcg
     1561 cgaacggcag cgtcagccgg ccgccacgct atcgggcggc gagcagcaga tgctggcgat
     1621 cgcccgcgcg ctgatgatga agccgaaact gctgatcctg gacgagccga cactcggcct
     1681 ggcaccggtg atcctggaac agctgtccaa ggcactggcc ctgctgcgcg aaaccacgcc
     1741 gatcaccgtg ctgctgggcg aacagaacgt caccttcgcg ctgccgcatg ccgaccgcgt
     1801 ctacgtgctg gaacatgcgc gcatcacctg ggaaggcgat ccgggacgct tcgccgagga
     1861 gatgggcacc ggatatctgt agccggaacc agcccgatat cagtccgcgg cccggatcgt
     1921 ctgccgcagc accgggtaga tctcccgctc ccaccgccgg ccgctgaaca cgccgtaatg
     1981 tccggcacgc gcctgctggt aatgcgtccg gcgctctgcc ggaatcgcgg tgcacagatc
     2041 atgcgccgca cgggtctggc cgatcgcaca aatgtcgtcg cgctcgccct ccacggtcag
     2101 caaggcggtg ccttcgatgg catccggccg tacccgctcg ccgcgccagt gcagccggcc
     2161 ctgcggcagt tcgtgccgct ggaacacgcg ctcgacggtt tccaggaaga actccgccgg
     2221 caggtccatc accgcgcgat actcgccgta aaagcggcga tgcgcttcca cccggtcgtc
     2281 ttcgccgccg accagcgcac gcaactgccc gacatgcgcg tccacatggc gatccaggtt
     2341 catgctcatg aaagctgtca gctgcacgaa gccgggatag acccgccgca acgcgccgcg
     2401 gtaacgcagc ggcaccacgc cgatcagccg ccgctcgaac caatcgatcg gacgcgcctc
     2461 cgccagccgg ttgacctggg tcgggttga
//
LOCUS       DASSZH010000590         2679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_900787, whole genome shotgun sequence.
ACCESSION   DASSZH010000590 DASSZH010000000
VERSION     DASSZH010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900787"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VFN42_12785"
     CDS             complement(<1..326)
                     /locus_tag="VFN42_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997536.1"
                     /translation="MPFDGSEFIGTTIDAAALARQHTGVWWRVRRMARALLPGMRTSP
                     FGVPLAPPMSPEQTTVQVLQLARTLIEDERHWIQRRYETLDGRRCAVGALRSAARLLD
                     LRGQPNG"
     gene            complement(441..2039)
                     /locus_tag="VFN42_12790"
     CDS             complement(441..2039)
                     /locus_tag="VFN42_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stimulus-sensing domain-containing protein"
                     /protein_id="HET8997537.1"
                     /translation="MSPLMRRILLVNALPLLLLVAALLYLDQYQNGLLEAQVTALREQ
                     AKVFAGALGESAVSVKTPDNPKLIPDVARPLLRRLTEPTPDADAKLYAPDGTILADSR
                     VREGPGGAVNAEPLPPAVEHGPILSAIGWLYDKALSLLPHDRPITLSEIPGAGLDWQP
                     NVKEELRLNSSGQSPEMPPYIRRTKDNQLLVTVAEPVERDRHTVGIILLTREAREVDA
                     SLFVVRMSIMGLFGLALAVTVFLSWYLSLTIARPILRLARAAAEMREGQGRSGAVPPS
                     LLGRRDEVGALARTLSESAQALWTRMDAIERFGADVAHEIKNPLSSIRSAIETLRRVE
                     EPARQKQLLTIIAQDVVRLDRLISDVSDASRLDAELSRTPAEPVDVSPILRTLQELDE
                     ATRDAENDPRLVVDAPESGLMVMAVEGRLVQVLRNLVGNAQSFSPPRGKILLRARATG
                     AVVEISVEDEGPGIPEAKLEHIFDRFYSERPQGERFGQHSGLGLSISRQIVEALRGRI
                     SAENRRDAAGKILGARFVVRLPKA"
     gene            complement(2115..>2679)
                     /locus_tag="VFN42_12795"
     CDS             complement(2115..>2679)
                     /locus_tag="VFN42_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8997538.1"
                     /translation="ADLAVLDIKMPRMDGMELLQRLRQRSTMPVIFLTSKDEEVDELM
                     GLRLGADDYITKPFSQRLLIERIRALLRRNEASRAEGSGATPGGVLTRGDLVLDETKH
                     QCTWRGQDIQLTVTEFLLVKALAARPGMVKSRDQLIDAAYGENIYVDDRTIDSHIKRV
                     RKKFRGVDDNFDQIETLYGIGYRYKEH"
BASE COUNT          434 a          903 c          886 g          456 t
ORIGIN      
        1 ccattcggct ggccgcgcag atcgagcagg cgggccgcgc tgcgcagcgc accgacggcg
       61 cagcggcggc cgtccagggt ctcatagcgc cgctggatcc aatggcgctc gtcctcgatc
      121 agcgtgcgcg ccaactgcag cacctggacg gtggtctgct ccggcgacat gggcggggcg
      181 agcgggacgc cgaagggcga ggtccgcatg ccgggcagca aggcgcgggc catgcgccgc
      241 acccgccacc atactcccgt gtgctgccgc gccagcgccg cagcgtcgat cgtcgtgccg
      301 atgaattcgc ttccgtcgaa cggcattgga ccagtctccc gcagttaagt gacctacctc
      361 cagaacagca gcggcggccg cgtgtttgcc gatgacacga atgtgggcga ggaaactgtt
      421 ggtggaagac actccagggt tcacgccttc ggcagacgaa caacgaaacg cgcgccaaga
      481 atcttgccgg cggcatcccg ccggttctcc gcggaaatcc gcccgcgcag cgcctcgaca
      541 atctgccggc tgatcgacag gccaagcccg gaatgctggc cgaagcgctc accctgcgga
      601 cgttccgagt agaatctgtc aaagatgtgc tcgagtttgg cttcgggtat gcccggtcct
      661 tcatcctcga cgctgatctc caccacggca ccggtggcgc gcgcgcgcag caggatcttg
      721 ccgcgtggcg gactgaagct ttgcgcgttg ccgacgagat tgcgcagaac ctgtaccaga
      781 cggccttcaa cggccatgac catcagcccc gattccggcg catcgaccac cagccgtggg
      841 tcgttctccg catcgcgcgt cgcttcgtcc agttcctgca gggtacgcag gatcggcgac
      901 acatccaccg gctccgccgg ggtacgcgaa agttccgcat ccagcctgga ggcatcgctg
      961 acgtcgctga tcagccggtc cagccgcacg acgtcctgcg cgatgatggt caggagctgc
     1021 ttctgccgcg ccggctcctc gacgcgccgc agggtttcga tggcactgcg aatggaggac
     1081 agcgggtttt tgatttcatg cgccacgtcg gcaccgaagc gttcgatggc atccatgcgc
     1141 gtccacagcg cctgggcgga ttccgacagg gtgcgggcca gggcgccgac ctcatcgcgc
     1201 cggccgagca gcgatggcgg caccgcgccg gaacggccct gcccttcccg catctccgct
     1261 gccgcccggg ccagccgcag aatcggccga gcgatggtca gcgagagata ccaggagaga
     1321 aacacggtca ccgccagcgc aaggccgaac aaccccatga tcgacatgcg cacgacgaac
     1381 aggctggcat ccacttcgcg tgcttcgcgg gtcagcagga tgatgcccac ggtgtgccgg
     1441 tcgcgctcga ccggttcggc caccgtcacc agaagctggt tgtccttggt gcgccggata
     1501 tagggcggca tttcgggact ttgtccggag ctgttcagcc gcagctcctc cttgacgttg
     1561 ggctgccagt ccaggccggc gcccggaatc tcggacaggg tgatcggccg gtcatgcggc
     1621 agcagcgaca aggccttgtc gtacagccag ccgatggcgc tgaggattgg tccatgttcc
     1681 accgccggcg gcagcggttc ggcattcacc gcgccgccgg gtccttcgcg cacgcgcgaa
     1741 tccgccagga tggtgccgtc cggcgcgtac agcttcgcgt cggcatccgg tgtcggctcg
     1801 gtcagccggc gtagcagcgg acgcgcgaca tcgggaatca gcttgggatt gtcaggcgtc
     1861 ttgacgctga cggcgctttc gcccagcgcg ccggcgaaca ccttggcctg ctcgcgcaag
     1921 gcggtgacct gggcttccag caagccgttc tggtactggt ccagatacag cagggccgcg
     1981 accagcagca gcagcggcaa ggcattcacc agcaggatcc gccgcatcag cggcgacacc
     2041 cagcgttggc ggatgcgctt cggctgtggc ggcgccggcc ggcttacctc cgcgatgcgg
     2101 ctaccgtcgg gcaatcagtg ctccttgtag cggtagccga tgccgtacag cgtttcgatc
     2161 tggtcgaaat tgtcgtccac gccgcggaat ttcttacgca cgcgcttgat gtggctgtcg
     2221 atggtgcggt cgtccacgta gatgttctcg ccataggcgg cgtcgatcag ctggtcacgg
     2281 cttttcacca tgcccggccg cgccgccagc gctttcacca gcaggaactc cgtgacggtg
     2341 agctgaatgt cctggccccg ccaggtgcac tggtgcttgg tctcgtcgag cacgagatcg
     2401 ccgcgtgtca gcacgccgcc gggcgtcgcg ccggaacctt ccgcccggct ggcctcgttg
     2461 cggcgaagca gcgcgcggat gcgctcgatc agcaggcgct ggctgaacgg cttggtgatg
     2521 tagtcgtcgg cgccgagacg caggcccatc aattcgtcca cctcctcgtc cttggaggtg
     2581 agaaagatca ccggcatggt gctgcgctgg cgcaggcgct gcagcagctc catgccgtcc
     2641 atgcgcggca tcttgatatc cagcacggcg aggtcggcc
//
LOCUS       DASSZH010000591         3328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_883408, whole genome shotgun sequence.
ACCESSION   DASSZH010000591 DASSZH010000000
VERSION     DASSZH010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883408"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VFN42_12800"
     CDS             complement(<1..763)
                     /locus_tag="VFN42_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997539.1"
                     /translation="MSDSEEDEPTPAQGGKKEAKEIWKRRALAAVNASEEIATTLLPY
                     GGGQSQVLLNLPDDYAERGQEERQRKRDRNADTRKKNRAKRQRTRQSELEPLAKTLIE
                     QLFEGLPAQIRNPRDFTGDRERQVLRWTATSLLTMLPGGVEVQCYYENGTVYLTSNDV
                     ATNRKLQALLEEHRGNVVAAITESYEAIAKQGARQERHAKKLIGRAQSRVYAAQDPIL
                     DCLTAGKVIVVQGSEEGLHAERRIQRLLQRKLDPRF"
     gene            complement(788..1258)
                     /locus_tag="VFN42_12805"
     CDS             complement(788..1258)
                     /locus_tag="VFN42_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997540.1"
                     /translation="MEPDMAARQAWIIRVLGFDPPRGNQSQSSTGSKAKPDLLAIWRE
                     AKDAVDVRLGALRRALLDEDDPDFARLADSGLFALGTRQNVTLMASLTAFRGAPPDAL
                     AEPAARVREAVRAYGELLATSPIPQVVDENPFGVDVKLRATLGDALARIVAATR"
     gene            complement(1352..2572)
                     /locus_tag="VFN42_12810"
     CDS             complement(1352..2572)
                     /locus_tag="VFN42_12810"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8997541.1"
                     /translation="MSATHIIVGAGQAGGWAAMAMRQAGFTGRILLIGEERWRPYERP
                     PLSKAVLTDDPEPDVLYFHAESRYAEQQIELRLGTAVAAIEPDQHRVHMADGSVLAYD
                     KLLLAVGGQARRLPVHGGDRALYLRTLEDARLIRERLATAKHVVCIGAGVIGLEIASS
                     ARKRGCAVTVLEALPRAMGRAVSPEGAAFIESLHRAAGVDLRFQVIVDRLEPSTDDGT
                     CVICRDGQRFTGDLVIAGIGMQRNLALAEAAGLALEGGIVVDEHGRTSAPDIYAAGDV
                     TAFFHPLFQHRLRLETWRHAQNQGAAVGKAMCGDPAPYDDIPWFWTDQHGVNLQVAGL
                     PAEAARTLVRSTTAPGAFVAVHLDETDRVIGVTAANNPREIRAGQALIKARQTVDAAA
                     LADGAIPLQRLIAR"
     gene            complement(2582..2935)
                     /locus_tag="VFN42_12815"
     CDS             complement(2582..2935)
                     /locus_tag="VFN42_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997542.1"
                     /translation="MLPVSGALAQVTLDAPGLLPKKPKPGPPPVKAKPPPWPRLDTGA
                     VLCRTPADLARLAAIRAGSAGGGPADCRVITQPTPITIVQRDGPGRTEVRLTGDGSDA
                     TGWTDVWLPHNPPSR"
BASE COUNT          517 a         1144 c         1091 g          576 t
ORIGIN      
        1 gaaaccgggg gtcgagtttc cgctgcagca gccgctgaat gcggcgctct gcgtgcaagc
       61 cttcctcgct gccctgaacg acaatgacct tcccggcggt aaggcagtcc aggatcgggt
      121 cctgcgccgc gtacacgcga ctctgcgccc ggccaatcag cttcttcgca tgccgttcct
      181 gccgcgcgcc ttgcttggcg atcgcttcat acgactccgt gatagccgcg acgacgttgc
      241 cacgatgctc ctccagcaag gcctgcagct tgcgattggt cgccacgtcg ttcgaggtga
      301 ggtagaccgt gccattctcg taatagcact gcacctccac gccgcccggc agcatcgtca
      361 gcaggctcgt ggcagtccaa cggagcacct ggcgctcacg atctccggtg aagtcgcggg
      421 ggttccggat ctgcgccggc aggccctcga acagctgctc gatcagcgtc ttcgccagcg
      481 gttccagttc ggactgacgt gtcctctgcc gcttcgcgcg attcttcttg cgcgtgtcgg
      541 cgtttcggtc gcgcttacgc tggcgttctt cctggccgcg ctcggcgtaa tcgtcgggca
      601 ggttcagcag cacctgcgac tggccgccac cgtaaggcag cagggtcgtg gcgatctcct
      661 cgcttgcatt gaccgcagcc aatgcgcggc gtttccatat ctccttcgct tccttcttgc
      721 cgccttgggc cggcgtaggt tcatcctctt cgctgtcgct catggcagtt tcctcaatac
      781 cgctgggtca gcgcgtcgca gcgacgatcc tggccagtgc gtcgccgagc gtggcgcgca
      841 gcttcacatc aacgccgaag ggattctcgt cgacgacctg cggaatcggg cttgttgcca
      901 gtaactcacc ataagcccga actgcctccc ggacgcgggc ggcgggttct gccagagcat
      961 ccggcggcgc gccgcgaaac gcggtgagcg aggccatcaa cgtcacgttc tggcgggtgc
     1021 caagtgcgaa caatccggaa tcggcgaggc gggcgaagtc gggatcgtcc tcgtcgagca
     1081 aggcccggcg cagcgcgcca agccgaacgt caaccgcgtc cttcgcttcc cgccagatcg
     1141 ccagcaggtc gggcttcgcc ttgctgccgg tggaggattg ggactgattg ccgcgcggcg
     1201 gatcgaagcc cagcacccgg atgatccagg cttgccgcgc cgccatatcg ggctccatgc
     1261 gcctgcactc ccccccgcag atgcagcaca cgataatcgc gcaactgtgg attgcacagt
     1321 gtttcttatg cgcacactgc cgttggggcg gttagcgcgc gatcagccgt tgcagcggaa
     1381 tggcgccatc cgccagtgca gcggcatcca cggtctggcg cgctttgatc agtgcctggc
     1441 cggcgcggat ttcccgcggg ttgttcgccg cggtcacgcc gatcacgcgg tccgtctcat
     1501 ccaaatgcac cgcgacgaag gcgcccggag cggtggtgct gcgcaccaag gtacgggctg
     1561 cttccgcggg cagtccggcg acctgcaggt tcaccccgtg ctggtcggtc cagaaccacg
     1621 ggatgtcgtc ataaggcgcc gggtcgccgc acatcgcctt gcccaccgcg gcgccctggt
     1681 tctgcgcgtg gcgccaggtt tccagccgca gccggtgctg gaacagcgga tgaaagaacg
     1741 cggtgacgtc gcccgccgcg tagatatccg gcgcactggt gcggccgtgt tcgtccacca
     1801 cgatgccgcc ttccagcgcc agccctgcgg cttctgccag cgccagattg cgctgcatgc
     1861 cgatgccggc gatcaccagg tcgccggtga agcgctggcc gtcgcggcag atgacgcagg
     1921 tcccgtcatc cgttgacggc tccagccggt ccacgatgac ctggaagcgc aaatcgacac
     1981 cggcagcccg atgcagcgat tcgatgaagg cggcgccctc cggtgacacc gcgcggccca
     2041 tggcgcgcgg cagcgcttcc agcactgtca ccgcgcagcc ccgcttgcgg gcggaggagg
     2101 cgatctccag tccgatcacc ccggcaccga tgcagacaac gtgtttcgcc gttgccagcc
     2161 gttcacggat cagccgggcg tcttccagcg tgcgcagata cagcgcgcgg tcgccgccat
     2221 gcaccggcag ccggcgggcc tggccgccga ccgcgagcag cagcttgtcg taggcgagca
     2281 cactgccgtc ggccatgtgg acgcggtgct ggtccggctc gatcgccgcc accgcagtgc
     2341 ccaggcgcaa ttcaatctgc tgttcggcgt agcgggactc cgcgtggaaa tacagtacgt
     2401 ctggttccgg gtcgtcggtc agcaccgcct tggacagggg cgggcgttca taggggcgcc
     2461 accgctcctc gccgatcagc agaatgcggc cggtaaatcc ggcctggcgc atggccatgg
     2521 ccgcccaacc gcccgcctgg ccggcaccga caatgatgtg ggtcgcgctc atccgccgat
     2581 gtcagcgaga gggcgggtta tgcggcagcc agacgtccgt ccaaccggtg gcgtcggaac
     2641 cgtcaccggt aagccgaacc tcggtgcgcc cggggccgtc gcgttgcacg atggtgatcg
     2701 gcgtgggttg cgtgatgaca cggcagtcgg ccgggccacc accggcgctg ccggcgcgga
     2761 tggcggcgag ccgggccaga tcggccgggg tccggcagag aacggcgccg gtatccagcc
     2821 gcggccaggg cggcggcttg gccttcacgg gcggcggtcc gggcttcggc ttctttggca
     2881 gcaggcctgg tgcatccagc gtgacctgcg ccagcgcgcc ggagaccggc agcatgcagg
     2941 cgagacacac agcaacgaca gccaatgcac gcattcacca gtcccgatga aggtttcccc
     3001 ggtcatgtgt atggaactcc ctcgccaggg aagcaaccgg gatgtcaatc gcacggcgcc
     3061 ttgtccgggt cggtcgcgcg cttgctgcgg catggcgcct gtgaccaaca tcggcatcgc
     3121 gcgagcaacg gcggagaagg gcttgcgccc cggtcacgaa ccggtgaaca tccgaagcct
     3181 tcatcacctg gaatcgtccg actccgctga tgcttcgcat ttccggcacc gttgcggcgc
     3241 caaagcctgt gaggctgaca cacccgcgag ccatgctgcg gcgccgggat aacgtgccca
     3301 tcttagctgc attcacttca gtggcaga
//
LOCUS       DASSZH010000592         2748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_116437, whole genome shotgun sequence.
ACCESSION   DASSZH010000592 DASSZH010000000
VERSION     DASSZH010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116437"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..393
                     /locus_tag="VFN42_12820"
     CDS             127..393
                     /locus_tag="VFN42_12820"
                     /inference="COORDINATES: protein motif:HMM:NF025140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4168 domain-containing protein"
                     /protein_id="HET8997543.1"
                     /translation="MQVSDDMVAKVGAAVGQVAMIRQEYTERVQSAGDDGERDRLAEK
                     AQQAAVKAISDQGISVSDYNEVVSAAENNPELEDRLIMAARAST"
     gene            complement(603..1742)
                     /locus_tag="VFN42_12825"
     CDS             complement(603..1742)
                     /locus_tag="VFN42_12825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET8997544.1"
                     /translation="MPSDQPESAPLLLHVFSTFAVGGAQIRFITLANHFGPAWRHRII
                     ATDGNYAGRDRLASQVNANCMQVEMRKGDTLGNVRRFRRVLRTVRPDMLITYNWGTIE
                     WAFANALPLVPHLHIEDGFGPEEQSAQLPRRVWLRRLFLRRATVMVPSRTLYRIATEV
                     WRLPRKHVLYVPNGIDLDRYGADASGSPDWPGEGPVIGTVATLRPEKNLPRLLRAVQL
                     LRTRMPVRLVIVGDGSERSSLEELAQTLGIAEAVHFTGYCATPNMMYRGFDVFALTSD
                     TEQMPLSVLEAMASGLAVVATDVGDVRDMVAEANLQFIVPRDEAALAGAMERLLQQPD
                     LRAAIGTANRAKAERDYDEDRMFRAHEALWSGRATPDDQAPQRAT"
     gene            1921..2454
                     /locus_tag="VFN42_12830"
     CDS             1921..2454
                     /locus_tag="VFN42_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HET8997545.1"
                     /translation="MDTESPGQSWHEITQAVLKRHNVSLVTYVPDNVLRPLIAGVHAD
                     PFFTAFAVPREEEGLGILAGAWMGGTRGILLMQTSGFATLPNVIASLAVPFQIPVLMM
                     VSERGTLGEFNLGQSLVARTMRPVLDALAIEHHTMTRLDEAEFILDRSITQAVATRAP
                     CCFILSPLLTGGKRFEK"
     gene            2464..>2748
                     /locus_tag="VFN42_12835"
     CDS             2464..>2748
                     /locus_tag="VFN42_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET8997546.1"
                     /translation="MSFAANHPVHRDQAKVMNRFDITQRMVARLKQEEAVIGGIGYTN
                     FDLWAASASPQGRRAQNFYMLGSMGLAVPIAVGVALAQPRRKVFALEGDGS"
BASE COUNT          503 a          940 c          886 g          419 t
ORIGIN      
        1 tcgtgaaccg ccccggttgc accccgttgc cgctcttacg atgtggttca aagcaacccc
       61 gagacagcaa tggagtgacc catggccggt gagcggaaag ccaaagccga ttcggaaaat
      121 cacgacgtgc aggtttctga cgacatggtg gcgaaggtcg gcgcagcggt cggtcaggtc
      181 gcgatgatcc ggcaggaata caccgaacgc gtgcagagtg ccggcgatga cggcgagcgt
      241 gaccgcttgg ccgaaaaggc gcagcaggcg gcggtgaagg caatcagcga ccagggcatc
      301 tccgtctcgg actacaacga ggtggtcagc gccgcggaga acaatccgga gttggaagac
      361 cgtctgatca tggcggcgcg cgcatcgacc tgaccgggac tgcggcgtgg tggcggccag
      421 tgggagacgc tcactgagcc gccaaacagc catgcgcggc gggcgcagac ccgccacgca
      481 tgatcggctg cggaagactc ccatgtcagc ttgtggcgct ggacctccct ggcaggaggg
      541 ccgcgagggg ggcctccgca gggcgtcgtc attgtagtgg acgaccgcca ccggcggcgc
      601 ttctacgttg cccgttgcgg cgcctgatcg tccggcgttg cgcgcccgct ccacagagct
      661 tcatgcgcgc ggaacatgcg gtcctcgtca tagtcgcgtt cggccttggc gcgattcgcg
      721 gtgccgatgg ccgcgcgcag atccggctgc tgcaacagcc gctccatcgc ccccgccaag
      781 gccgcctcgt cgcgcggcac aatgaactga agatttgctt cggcgaccat atcgcgcaca
      841 tcgccgacat ccgtggccac caccgccagg ccggacgcca tcgcttccag cacggacaac
      901 ggcatctgtt cggtatccga cgtcagagcg aatacatcga agccgcggta catcatgttc
      961 ggcgtcgcac agtatccggt gaaatgcacc gcctcggcga tgccgagcgt ttgcgccagt
     1021 tcctcgaggg aggagcgttc ggagccgtcg ccaacgatca ccagccgcac cggcatgcgc
     1081 gtgcgcagca attgcaccgc gcgcagcagg cgcggcaggt tcttctcagg gcgcaacgtc
     1141 gccacggtgc cgatcaccgg cccttcgccg ggccagtccg gcgacccgct ggcatccgcg
     1201 ccgtagcggt cgaggtcaat cccgttcggc acatacagca catgcttgcg cggtagccgc
     1261 cacacctcgg tcgcgatgcg gtacagcgtg cgcgacggca ccatcactgt cgcccggcgc
     1321 aggaacagcc ggcgcaacca gacacggcgg gggagctgtg cgctctgctc ctccggacca
     1381 aatccgtctt cgatatgcag gtgcggcacc agtggcagcg cattggcgaa ggcccactcg
     1441 atggtgcccc agttgtaggt gatcagcatg tccggccgga cggtgcgcag cacccggcga
     1501 aagcggcgca cattgcccag cgtgtcgcct ttccgcatct ccacctgcat gcaattcgca
     1561 ttgacctgcg atgccagccg gtcacgaccg gcgtagttgc cgtctgtcgc gatgatccgg
     1621 tgccgccagg cagggccgaa gtgattagcc agagtgataa agcggatctg cgccccgccc
     1681 acggcaaatg tcgagaacac gtgcagcagc agaggggcgg actccggctg gtcagacggc
     1741 atgcatggcg atcccggaaa atgccgcaac ctgcgggtac gagcggaaat gccgggatcg
     1801 tacaacgccg accgttgcga taccagcagc gcagaccacc cgcccgcccc ccgccggttg
     1861 cccaacccgc caactcgtga caaacgccct ccaccgcccc gaagcagcgg aggaaacccc
     1921 atggataccg agtccccggg ccagtcctgg cacgaaataa cccaggcggt gctgaaacgg
     1981 cacaatgtca gcctggtcac ctatgttccc gacaacgtcc tccgcccgct gatcgccggc
     2041 gtgcatgccg acccgttctt caccgccttc gcggtgccgc gcgaggagga aggcctgggc
     2101 atcctcgccg gcgcctggat gggcggcaca cgcggcatcc tgctgatgca gaccagcggc
     2161 ttcgccacgc tgccgaatgt catcgcgtcg ctggcggtgc cgttccagat cccggtgctg
     2221 atgatggtgt ccgaacgcgg cacgctgggc gaattcaacc tcggccagtc gctggtcgcc
     2281 cgcaccatgc gcccggtgct cgacgcgctg gcgatcgagc accacaccat gacccggctg
     2341 gacgaggcgg aattcatcct ggaccgcagc atcacccagg cggtggcaac ccgcgcgccc
     2401 tgctgcttca tcctctcgcc cctgctgacg ggcggcaagc gcttcgagaa ataggggcac
     2461 gccatgtcat tcgcagcaaa ccatccggtg caccgcgacc aggccaaggt gatgaaccgc
     2521 ttcgacatca cccagcgcat ggtggcccgc ctgaagcagg aagaagccgt gatcggtggc
     2581 atcggctaca ccaatttcga cctgtgggcc gcctccgcct cgccacaggg cagacgcgca
     2641 cagaacttct acatgctggg cagcatgggg ctggcggtgc cgatcgccgt cggcgtcgcg
     2701 ctggcgcagc cgcggcgcaa ggtatttgcg ctggaaggcg acggctcc
//
LOCUS       DASSZH010000593        11104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_713322, whole genome shotgun sequence.
ACCESSION   DASSZH010000593 DASSZH010000000
VERSION     DASSZH010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 11104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11104
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713322"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..622)
                     /gene="pcaG"
                     /locus_tag="VFN42_12840"
     CDS             complement(101..622)
                     /gene="pcaG"
                     /locus_tag="VFN42_12840"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HET8997547.1"
                     /translation="MPIATASQTIGPYWHLIEDKDWADLTRFGAEGEKITLTGRVLDG
                     DGLPCADACVEIWQADPPASDSFPGFGRSVADADGAFRFVTLKPGPVPGRGNAQQAPH
                     IAICILSRGLMKGLVTRAYFHGEALNESDPLLLSLDDPARRATLIARPDGPGVWRLDI
                     RLQGDGETVFLEV"
     gene            complement(624..1328)
                     /gene="pcaH"
                     /locus_tag="VFN42_12845"
     CDS             complement(624..1328)
                     /gene="pcaH"
                     /locus_tag="VFN42_12845"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HET8997548.1"
                     /translation="MSDPVGYRPYAPGTQPSYDVPAYRGTTKRHPKQALHPLPPTITE
                     TSGPRFSPARFPASADLTSNTTGKPALGERIIVAGRVLDEDGRPVRNTMVEIWQCNAT
                     GRYDHPADQHDAPLDANFHGAGRVFTDEDGNYRFLTIRPGAYPWPNHHNAWRPPHIHY
                     SLFGPAFATRLVTQMFFLGDPLLAFDPIFNGVPDAHARNRLIAQFDLELTQPEYALGY
                     RWDIVLRGSAATPMEE"
     gene            1464..2333
                     /locus_tag="VFN42_12850"
     CDS             1464..2333
                     /locus_tag="VFN42_12850"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET8997549.1"
                     /translation="MLPTERLRYSAITERPPLRLPGGARVAVWVIVNVEEWDPRETMP
                     RTVLTPPAGGVPTPDIPNWAWHEYGNRVGFWRMLAVFDRLAIPATLAINGSAIAAYRP
                     IAQAALDRNWEFIGHGYGQKNMQKVPDERADIARTRETIRGFTGRAPRGWLGPGLTET
                     WETPDLLAEEGYDYVCDWVLDDQPVELRTRGKPIINIPYTQECNDVAMMLIQHHKAAE
                     YRDRAIDQFEQIHADAQDSARVMALVVHPYIMGAAHRLRYFREALAHIHARGDVVFQT
                     GEQILDWYLQART"
     gene            complement(2346..3218)
                     /locus_tag="VFN42_12855"
     CDS             complement(2346..3218)
                     /locus_tag="VFN42_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET8997550.1"
                     /translation="MAQQIPVDPAAQVPVPDDPVLEVLADLAYRRLAMVNVALVGQAH
                     AGAGRWVLVDAGLPGTAGLIQRAAAERFGRGAAPAAIVLTHGHFDHVGALRTLAEQWN
                     VPIYAHPLEHPYLNGSDSYPAPDPLVGGGLMSLAAPLLPRGPIDVTRWLRALPQDGSV
                     PLLPGWRWLHTPGHAPGHVSLWREADRTLLAGDAFITTAQESAYAVAVQEPELHGPPM
                     YYTPDWELAAGSVRRLAALEPELVITGHGRAMHGPAMRQALHRLADNFEQVAVPRHGR
                     YAGHPVQPGRRPGD"
     gene            complement(3221..3793)
                     /locus_tag="VFN42_12860"
     CDS             complement(3221..3793)
                     /locus_tag="VFN42_12860"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HET8997551.1"
                     /translation="MDRPLEIAFHNLDSSPTVEAEIRERVDKLATRYRHLTGCRVSVE
                     KLHNQHRTGNLFEVHIVLSVPGRDLAVSREPQKAKQRYAHPDIRSSIRDAFGAAERQL
                     ESYKGQLREDTAAPSASALAGRVMLIEPGVDHGFIETPAGTQIYFHRDSVTNARFEDL
                     KEGDAVHYVAEEGDAGPVATKVRIPEATEH"
     gene            3949..5091
                     /gene="lptF"
                     /locus_tag="VFN42_12865"
     CDS             3949..5091
                     /gene="lptF"
                     /locus_tag="VFN42_12865"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007282807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HET8997552.1"
                     /translation="MRTGRVITRLDIYIFRQLALALIAVTGGLAALVWLTQSLRFVEL
                     VVNHGLSFGVFLRLTGLLVPSLIAVILPITTFIVTQFVYQRLAGDRELTVMRAAGLSP
                     LTLARPALVLATLAVIACYVLNLWIVPLSFTAFRNYQWEIRNRVAAYLLQDGVFTPIT
                     NNLTIYIRSREPDGTLHGILVDDSRDKSAPATVLAETGRLIQGPNGPRVLLLNGSREV
                     IDQQTGRLDLLTFKQNEIDLSDQTHNKAARTADMSEVSLYTLLHPHPANPADAPRWIA
                     EAYKRLSSPLTAMSYALIALLSVLTGTFRRLGSFVRPLVAIGAVVLLLAAGLAINNLA
                     DRNNALIPLMWVQAGLPGVICGWLLLAPQLLGALRRPLALRDEPAG"
     gene            5096..6208
                     /gene="lptG"
                     /locus_tag="VFN42_12870"
     CDS             5096..6208
                     /gene="lptG"
                     /locus_tag="VFN42_12870"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019090094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HET8997553.1"
                     /translation="MNFAVTLSWYITRQFALWVVAMLFGLTGLVSLFDFLELLRRAAN
                     KPDVTLGLVTEVAALRLPFIAMQVLPFAVLLGGMLCFWRLTRSSELIVARAAGVSAWQ
                     FLAAPTACALLFGALTTTAGSPLSSVMFARANTLENEYLSTGGGPLALHGGQLWLRQA
                     DRALVPKGVAIIHANDVSLHGKRLTARNVSVFRLNNQDQLLARIEAARAVLGRGDWHL
                     EGARTVRPDTAPTQPQTINLPTDLTVNRVQDSFAAPDTLSVWALPDFIALLNRSGFSS
                     IRHRLHFQALLALPLLAATMALVGAGFSMRPARRGGVARMIGGGVAAGFALFMISKLA
                     EEFGQSGALPVMLAAWAPAGAGLLMALTLLLHMEDG"
     gene            6211..8430
                     /gene="lptD"
                     /locus_tag="VFN42_12875"
     CDS             6211..8430
                     /gene="lptD"
                     /locus_tag="VFN42_12875"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HET8997554.1"
                     /translation="MKRAILATLLPALLLAAPAQAQLGGAIRPSGPPITRNQPVSFTA
                     DQVEYDRPNNLVVASGHVEAWQNGHVLRADRIVFNRSTGTAAATGNVVLVEPGGEVLF
                     ADYVELTDNMRDAVLKDMRARLAENGKLAANGARRIGGVLNELSKVVYSPCNLCLKNP
                     SKPPFWQVRAASATEDLQHKRIEYRDAELQMFGVPVAYTPYLSMPDPSVKRSSGLLMP
                     GAGVSSHLGAFAYVPYYWAIDGESDATFTPMMTTRSGPQLEAEYRHRFNSGYFSFDGT
                     AGYLENSPQGTLSTQGQFDYNDTWRWGFDINRASSADYIRNFRVGDYYHGQTNVLASQ
                     IYAEGFGQGSYARLDTRFYQGLAGTISTAQLPIVLPRYQYSYFGRPDALGGRLTLDAD
                     AFNVLRSQGTDTRRARLTVNWERPFTGRLGDLWTVTVHADAMGYEAQQFNEQPNFGPR
                     SRINATRALPQIAVDWRWPFMRDSGKWGVQVIEPHLQLIAAPTVGDSQWSRYPNEDSL
                     DINYTTSNLFSFNRFSGVDRLEGGTRLNAALQGTWYLGGTTFDGMIGQSYRAAKDTSF
                     PAWSGLRDQVSDIVARLSFAPTGWLDVTYRTRLDHRTLQTRMADAVVALGVPAFNVTA
                     GYIYTPYNPYTLYNQANPSPDMPPAGSAFYTPRNEIVLGANAKWGDYRLSGYVQRSLS
                     TNQMVAAGANAAYENECFIFNILLFRRYTSIDNDHGSTALLFQFTFKTLGQIGYRAL"
     gene            8448..9785
                     /locus_tag="VFN42_12880"
     CDS             8448..9785
                     /locus_tag="VFN42_12880"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010502004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET8997555.1"
                     /translation="MRKSLVRARLAAVAVAAALALPGASAWAAPVRPKPAAAVPAATE
                     QTRIAAVVNGDVISNVDVENRARLFAMSTGLPLTPEVLDRLKPQMLHQLVDERVRLQE
                     IERRHIVVPDKQIAAAIREIEARNGMPAGALMARLKKAGISPLTLIDQIRAQLGWTLV
                     LRQELGEQARITDSEVAARQRALQQDVGKPEYRVGEIFIPIDSPSGRADAQHFAETVI
                     KELRAGASFPLVAAQFSQSQSALHGGELGWVQPNQLDPEVAKLVQQMPVGAISNPIPV
                     PGGLTIATLQGKRLIGRDMATILSVRQDFLPFSTTLNPQDPTAQQRAQLDKARHISQT
                     VHSCAQMEQQAKDDHSPRPVDPGPVRLETVNPPVFRQLLGTIPIGQPTKPLVAVDGIV
                     VMAVCSREQKNLADENAQQVREQLLAERVDNASRQLQHELQRRAQISIRQGST"
     gene            9800..10702
                     /locus_tag="VFN42_12885"
     CDS             9800..10702
                     /locus_tag="VFN42_12885"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET8997556.1"
                     /translation="MSASAAAQAGQRRAAVLADRVVPLLFVLLWSSSFIAARVGLRQM
                     TPLLFVALRMVLCAVVLVSVMLVLRRSWSVLRGWVWFHCAVAGVLANGVLLMTAHVAM
                     VHTQAAPIALIQTLNPLLTAVLAWPLLGERLRPQQWIGLLLGLTGVVLILGLAALHSR
                     TQFGGLLLTAGGVLGLVAGTLYFGRFCRGVPLLEGTTVQFTACAVACVACMAIFETPH
                     ATWTPGAVAAIAWNAAFVSLGGMVLYFLMLKRGAAARAAANFYLVPGTAAVLAWVLLG
                     EALSALTILGLVISSVGCWLVNRR"
     gene            complement(10763..>11104)
                     /locus_tag="VFN42_12890"
     CDS             complement(10763..>11104)
                     /locus_tag="VFN42_12890"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET8997557.1"
                     /translation="VLLDRARRNEAAGLSPEAATREAVRVRFRPILLTTLTAVLGMLP
                     TALGWGKGAAPEQGLALVILGGIVGSAMLSTNLLPALYIHWRRRPPGRHSAGSKTRDA
                     RSAKSRFAGVP"
BASE COUNT         1703 a         3933 c         3707 g         1761 t
ORIGIN      
        1 agggtgctat cgaggttgcc cggggaacgc ctgaacgccc cctccccccg ccccctcccg
       61 caaggggagg gggcgctcct acaccccgcc ccttcgaaaa ctacacctcc aggaacaccg
      121 tctccccgtc gccctgcagc cggatatcca gccgccacac ccccggcccg tccggccgcg
      181 cgatcagcgt cgcccgccga gcgggatcgt ccagcgacag cagcagcggg tcgctctcgt
      241 tcagcgcctc gccgtggaaa taggcgcgcg tcaccagccc cttcatcagc ccgcgcgaca
      301 ggatgcagat ggcgatatgc ggcgcctgct gcgcgttgcc gcggcccggc accgggcccg
      361 gcttcagcgt cacgaaacgg aacgccccgt cggcatccgc cacgctgcgg ccgaagccgg
      421 gaaagctgtc ggaggccggc ggatcggcct gccagatctc cacacaggca tcggcgcagg
      481 gcaggccgtc gccgtccagc acccggccgg tcagcgtgat cttttcacct tccgcgccga
      541 accgcgtcag gtcggcccag tccttgtcct cgatcaggtg ccagtacggg ccgatggtct
      601 ggctggcggt cgcgatcggc atgctactcc tccatcggcg tggcggcgct gccgcgcagc
      661 acaatgtccc agcgatagcc cagtgcgtat tccggctgcg tcagctccag gtcgaactgc
      721 gcgatcagcc ggttgcgcgc atgcgcatcg ggcacgccgt tgaagatcgg gtcgaacgcc
      781 agcagcggat cgccgaggaa gaacatctgc gtcaccagcc gggtggcgaa ggccgggccg
      841 aacagcgagt agtggatatg cggcggccgc caggcattgt ggtggttcgg ccagggatac
      901 gcaccgggcc ggatcgtcag gaaccggtag ttgccgtcct catcggtgaa cacccgccct
      961 gcaccatgga aattcgcatc cagcggtgcg tcgtgctggt cggccggatg gtcgtagcgg
     1021 ccggtggcgt tgcactgcca gatctccacc atggtgttgc gcaccggccg gccatcctca
     1081 tccagcacgc ggcccgccac gatgatgcgc tcgcccagcg ccggcttgcc cgtggtgttg
     1141 ctggtcaggt cggcggaggc cgggaaccgc gccggcgaga agcgcggacc gctggtttcc
     1201 gtgatggtgg gcggcagcgg atgcagcgcc tgtttcggat gccgcttggt ggtgccgcga
     1261 taggccggca cgtcgtagga cggctgcgtg ccgggcgcgt agggacggta gccaaccgga
     1321 tcgctcatgc attgcgctcc ctgaatgggt gccgcaccgt ctcacgccgc ccgggccggg
     1381 gcaaccggac atggtccggc aaacgcgccg gcgcagccgg atcagccttg cgccgggccg
     1441 gcgccgatac ggaggagatc gccatgctgc ccaccgagcg cctgcgctat tccgccatca
     1501 ccgagcgccc gccgctccgc ctgccgggcg gcgcccgggt cgcggtctgg gtcatcgtca
     1561 acgtggaaga atgggacccg cgcgagacca tgccgcgcac cgtgctgacc ccgcccgccg
     1621 gcggcgtgcc gacgccggac atccccaact gggcctggca tgaatacggc aaccgcgtcg
     1681 ggttctggcg catgctggcg gtgttcgacc ggctggccat tccggccacc ctggcgatca
     1741 acggctccgc catcgcggca taccggccga tcgcgcaggc cgcgctcgac cgcaactggg
     1801 agttcatcgg ccacggctac ggccagaaga acatgcagaa ggtgccggac gagcgcgccg
     1861 atatcgcccg gacccgcgaa accatccgcg gcttcaccgg ccgagcgccg cgcggctggc
     1921 tagggcccgg cctcacggaa acctgggaaa cccccgatct gctggccgag gaaggctacg
     1981 actacgtctg cgactgggtg ctggacgatc agccggtgga actgcggacc cgcggcaagc
     2041 cgatcatcaa catcccgtac acgcaggaat gcaacgacgt cgccatgatg ctcatccagc
     2101 atcacaaggc ggcggaatac cgcgaccgtg cgatcgacca gttcgaacag attcacgccg
     2161 acgcccagga ctccgcgcgg gtgatggcgc tggtggtcca cccctacatc atgggggcgg
     2221 cgcaccggct gcgctatttc cgcgaggccc tggcgcacat ccacgcccgc ggcgacgtgg
     2281 tgttccagac cggcgagcag atcctcgact ggtatctgca ggcacgcaca tagccgcgcc
     2341 gggagctagt caccgggacg cctgcccggc tgcaccgggt ggccggcgta gcggccatgc
     2401 cgcggcaccg ccacctgttc gaaattgtcc gccagccgat gcagcgcctg ccgcatcgcc
     2461 ggaccatgca tggctctgcc atgcccggtg atcaccagct cgggctccag cgccgccagc
     2521 cggcgcaccg agccggcggc cagctcccag tccggcgtgt agtacatcgg cggaccgtgc
     2581 agctccggct cctgcaccgc cacggcatag gcggattcct gcgccgtggt gatgaacgcg
     2641 tcgccggcca gcagcgtgcg gtccgcttcc cgccacagcg acacgtggcc cggggcgtgc
     2701 cccggcgtgt gcagccagcg ccagcccggc agcagcggca cgctgccgtc ctgcggcagc
     2761 gcgcgtagcc agcgggtcac atcgatcggt ccgcgcggca gcaaaggggc ggcgagcgac
     2821 atgagaccgc cgcccaccag cgggtccggc gcaggatagg agtcggagcc gttcaggtac
     2881 ggatgctcca gcggatgcgc gtagatcggc acgttccatt gctccgccag cgtgcgcagc
     2941 gcgccgacat ggtcgaaatg cccgtgcgtc agcacgatcg ccgccggggc tgcgccgcgg
     3001 ccgaaccgct ccgccgccgc ccgctggatc agcccggcgg tgcccggcag cccggcgtcc
     3061 accagcaccc agcgcccggc gccggcatgc gcctggccca ccagcgccac gttgaccatc
     3121 gccagccgcc ggtaggcgag atccgccagc acctccagta ccggatcgtc cggcacgggc
     3181 acctgggcgg cgggatcgac gggtatctgt tgcgccatgc tcagtgttcc gtcgcttccg
     3241 ggatgcgcac cttggtggcg accgggccgg catcaccctc ctcggcgacg tagtgcacgg
     3301 cgtccccctc cttcaggtcc tcgaaccggg cattggtgac gctatcgcga tggaagtaga
     3361 tctgcgtgcc ggccggggtt tcgatgaagc cgtgatcgac gcccggctcg atcagcatca
     3421 cccggccagc cagggcggac gcgctcggcg ccgcggtgtc ctcgcgcagt tggcccttgt
     3481 aggattccag ttgccgctcc gccgcgccga acgcatcgcg gatcgagcta cggatatcgg
     3541 gatgcgcata gcgctgcttc gccttctgcg gctcgcgaga gaccgccagg tcccgcccgg
     3601 gcaccgacaa cacgatatgc acctcgaaca ggttgccggt gcggtgctgg ttgtgcagct
     3661 tctccaccga cacccggcag ccggtcaggt ggcggtagcg ggtggccagc ttgtccaccc
     3721 gctcgcggat ctccgcctcc acggtgggag agctgtccag gttgtgaaac gcaatctcca
     3781 gcggtcggtc catcgggcaa gctccgtcat gccgcttctc tgcccatgac aacagagaac
     3841 cgcagcctac ggttgcgccc ccggacgccg cccgcggtcc atgaacccgg ttgcatcccc
     3901 tgcccggctg ccgcagaaac gccgggcaac aggagaccgc cgcccctcat gcgcaccgga
     3961 cgggtcatca cccggctcga catctacatt ttccggcagc ttgccctggc cctgattgcg
     4021 gtcaccggcg ggctggccgc gctggtctgg ctgacccagt cgctccgctt cgtcgagctg
     4081 gtggtcaatc acggcctgtc cttcggcgta ttcctgcgcc tgaccggcct gctggttccc
     4141 agcctgatcg cggtgatcct gccgatcacc accttcatcg tcacccagtt cgtctatcag
     4201 cggctggccg gtgaccggga gctgacggtg atgcgcgcgg ccggcctgtc gccgctgacg
     4261 ctggcccgcc cggcgctggt gctggccacc ctggcggtga tcgcctgcta cgtgctgaac
     4321 ctgtggatcg tcccgctcag cttcaccgcg ttccgcaact accaatggga aatccgcaac
     4381 cgcgtcgccg cctacctgct gcaggacggg gtgttcacgc cgatcaccaa taacctgacg
     4441 atctacatcc gctcccgcga gccggacggc acgctgcacg gcatcctggt ggacgacagc
     4501 cgggacaaga gcgcgccggc cacagtcctg gccgagaccg gccggttgat ccaggggccg
     4561 aacggccccc gcgtgctgct gctcaacggc agccgcgagg tgatcgacca gcagaccggc
     4621 cggctggacc tgctgacctt caagcagaac gagatcgacc tctcggacca gacccacaac
     4681 aaagccgccc ggactgccga catgtcggag gtctcgctct acaccctgct gcatccgcat
     4741 ccggccaacc cggccgacgc gccgagatgg atcgccgagg cctacaaacg gctcagcagc
     4801 ccgctcaccg ccatgtccta cgcgctgatc gcactgctgt cggtgctgac cggaaccttc
     4861 cgccgcctgg gcagcttcgt ccggccgctg gtggcgatcg gcgcagtggt gctgctgctg
     4921 gcggccgggc tggcgatcaa caacctggcc gaccgcaaca atgcgctgat cccgttgatg
     4981 tgggtgcagg ccggcctgcc cggggtgatc tgcggctggc tgctgctggc gccgcagctt
     5041 ttgggggcgc tgcgccgccc cttggcattg cgcgacgagc cggcgggctg atccggtgaa
     5101 cttcgccgtc acgctgtcct ggtacatcac ccggcaattc gccctctggg tcgtcgccat
     5161 gctgttcggg ctgaccgggc tcgtctcgct gttcgatttc ctcgaactgc tgcgccgagc
     5221 ggccaacaag ccggacgtga cgctgggcct ggtgacggag gtggcggcgc tgcggctgcc
     5281 cttcatcgcc atgcaggtgc tgcccttcgc cgtgctgctg ggcggcatgc tgtgcttctg
     5341 gcgactgacc cgctcctccg agctgatcgt cgcccgcgcg gcgggcgtct ccgcctggca
     5401 gttcctggcc gcgccgaccg cctgcgcgct gctgttcggt gcactgacca ccacggccgg
     5461 cagcccgctg tcctcggtga tgttcgcccg ggccaacacc ctggagaacg agtatctcag
     5521 caccggcggc ggaccgctgg cgctgcatgg cggccagctc tggttgcgcc aggccgaccg
     5581 cgcgctggtg ccgaagggcg tggccatcat ccatgcgaat gatgtgtcgc tgcacggcaa
     5641 gcggctcacc gcccgcaacg tgagcgtgtt ccggctgaac aaccaggacc agttgctggc
     5701 gcggatcgag gcggcccggg cggtgctggg ccgcggcgac tggcatctgg agggcgcgcg
     5761 caccgtgcgg cccgacaccg cgcccaccca gccgcagacg atcaacctgc cgaccgatct
     5821 cacggtcaac cgggtacagg acagcttcgc cgcccccgat acgttgtcgg tctgggcgct
     5881 gcccgacttc atcgcgctgc tgaaccgctc cggcttctcc tccatccggc atcggctgca
     5941 tttccaggcg ctgctggcac tgccgctgct ggccgcgacg atggcgctgg tgggcgccgg
     6001 cttctcgatg cgcccggcgc ggcgcggcgg ggtggcgcgg atgatcggcg gcggcgtggc
     6061 ggccggcttc gcgctgttca tgatctccaa gctggcggag gaattcggcc agtccggcgc
     6121 cctgccggtg atgctggccg cctgggcgcc cgccggtgcc ggactgctca tggcgctgac
     6181 cctgctgctg cacatggaag acggctaggc atgaagcggg ccatactcgc cactctgctc
     6241 ccggcattgc tgctggccgc gccggcccag gcacagttgg gcggcgccat ccggccatcc
     6301 ggcccgccga tcacccgcaa ccagccggtc agcttcaccg ccgaccaggt ggaatacgac
     6361 cggcccaaca acctcgtggt cgccagcggc catgtggagg cctggcagaa cggtcacgtg
     6421 ctgcgcgccg accggatcgt gttcaaccgc agcaccggca ccgcggcggc gaccggcaat
     6481 gtcgtgctgg tggaaccggg gggcgaggtg ctgttcgccg actatgtcga attgaccgac
     6541 aacatgcgcg acgccgtgct gaaggacatg cgcgcgcgcc tggcggaaaa cggcaagctg
     6601 gcggcgaacg gcgcgcggcg gatcggcggg gtgctgaatg agctgtcgaa ggtggtgtat
     6661 tcgccctgca acctgtgcct gaagaatccc tcgaagccgc cgttctggca ggtgcgcgcc
     6721 gcttcggcga ccgaggacct gcagcacaag cggatcgagt accgcgacgc ggaattgcag
     6781 atgttcggcg tcccggtggc gtacacgcca tacctgtcga tgccggatcc gtcggtgaag
     6841 cgctcctccg gattgctgat gccgggcgcc ggcgtgtcct cgcatctcgg cgcgttcgcc
     6901 tacgtcccgt attactgggc gatcgatggg gaatccgacg cgaccttcac gccgatgatg
     6961 acgacacgct ccgggccgca gctcgaagcg gaataccggc accggttcaa cagcggctat
     7021 ttcagcttcg acgggaccgc cggctacctg gagaactcgc cgcagggcac gctcagcacc
     7081 cagggccagt tcgactacaa cgacacctgg cgctggggct tcgatatcaa ccgtgcgtcc
     7141 tccgccgact atatccgcaa ctttcgcgtc ggggattact accacggcca gaccaacgtg
     7201 ctggccagcc agatctatgc cgaaggattc ggccaaggct cctacgcgcg gctggacacg
     7261 cggttctacc aggggcttgc cggcacgatc tccaccgccc agttgccgat cgtgctgccc
     7321 cgctaccagt acagctattt cggccgcccg gatgcgctgg gcgggcggct gacactggat
     7381 gccgacgcct tcaacgtgct gcgctcacag ggcaccgata cccggcgcgc gcggctgaca
     7441 gtgaactggg aacggccgtt caccggcagg ctgggcgatc tgtggaccgt gacggtgcat
     7501 gccgacgcga tggggtatga ggcgcagcag ttcaacgagc agccgaattt cggcccccgc
     7561 tccaggatca acgccacgcg cgcgctgccg cagatcgcgg tggactggcg ctggcccttc
     7621 atgcgggatt ccggcaaatg gggcgtccag gtgatcgagc cgcatctgca gctcatcgcc
     7681 gcgccgacag tgggcgacag ccagtggagc cgctatccca acgaagacag cctggacatc
     7741 aactacacca cgtcgaacct gttcagcttc aaccgcttct cgggggtgga ccggctggaa
     7801 ggcggcacaa ggctgaacgc ggcgctgcag ggcacctggt acctgggcgg caccaccttc
     7861 gacggcatga tcggccagtc ctatcgcgcc gccaaggaca cctccttccc ggcatggtcc
     7921 ggcctgcgcg accaggtgtc cgatatcgtc gcgcggctca gcttcgcgcc gaccggatgg
     7981 ctggacgtga cctaccgcac ccggctggat caccggacgc tgcagacccg gatggccgac
     8041 gcggtggtcg cactcggcgt cccggcgttc aacgtcaccg caggttacat ctatacgccg
     8101 tacaatccct acacgctgta caaccaggcc aacccgtcgc cggacatgcc gccggccgga
     8161 tcggcgttct acacgccgcg caacgaaatc gtcctgggcg ccaacgccaa atggggcgac
     8221 taccggttga gcggttatgt gcagcggagc ctcagcacca accagatggt ggcggccggc
     8281 gccaatgccg cgtatgagaa cgagtgcttc atcttcaaca tcctgctgtt tcgacgctac
     8341 acctcgatcg acaatgatca cgggtcgacg gcattgctgt ttcaattcac cttcaagacg
     8401 ctcgggcaga tcggttatcg tgccctgtag gagcgggagc cccggccatg cgtaagtccc
     8461 ttgtccgcgc ccgcctcgcc gctgtcgcgg tggccgccgc actcgccctg cccggcgcat
     8521 ccgcttgggc tgcgccggtg cgcccgaagc ccgccgccgc cgtaccggcc gccaccgagc
     8581 agacccggat cgccgcggtg gtgaacggcg atgtcatcag caatgtggac gtggagaacc
     8641 gcgccaggct gttcgccatg tccaccggcc tgccgctgac gcccgaggtg ctggaccggc
     8701 tcaagccgca gatgctgcac cagttggtgg acgagcgggt acggctgcag gagatcgagc
     8761 gccggcacat cgtggtgccg gacaagcaga tcgccgccgc gatccgcgag atcgaagcgc
     8821 gcaatggcat gcccgccggt gcgctgatgg cgcggctgaa aaaggcaggc atcagcccgc
     8881 tgacgctgat cgaccagatc cgtgcccagc tcggctggac cctggtgctg cgccaggagc
     8941 ttggcgagca ggccaggatc accgattccg aggtcgccgc ccgccagcgc gcgctgcagc
     9001 aggatgtggg caagccggaa taccgcgtcg gcgagatctt cattccaatc gacagtccat
     9061 ccggcagggc ggatgcacag catttcgccg agaccgtcat caaggaactg cgtgccggcg
     9121 cctctttccc gctggtggcc gcccagttca gccagagcca gtccgcgctg cacggcggtg
     9181 aactcggctg ggtgcagccg aaccagctcg atcccgaggt ggccaagctg gtgcagcaaa
     9241 tgccagtcgg cgcgatcagc aatccgatcc cggtgccggg cggactgacc atcgccacac
     9301 tgcagggcaa gcggctgatc ggccgcgaca tggcgacgat cctgtcagtg cgtcaggatt
     9361 tcctgccctt ctcgacgacg ctgaatccgc aggatcccac cgcacagcag cgggcacagc
     9421 tcgataaggc gcgccacatc agccagacgg tgcatagctg tgcgcagatg gaacagcagg
     9481 cgaaggacga ccattccccg cgccccgtcg atcccggccc tgtgcggctg gagacggtga
     9541 atccgccggt gttccgccaa ttgctgggaa ccataccgat cggtcagccc accaagccgc
     9601 tggtcgcggt cgatggcatc gtggtgatgg cggtctgctc ccgcgaacag aagaacctgg
     9661 ccgatgagaa tgcacagcag gtgcgcgagc aactgctggc agagcgggtc gacaacgcgt
     9721 cgcggcagtt gcagcacgaa cttcagcggc gggcacagat cagcatccgc cagggttcca
     9781 cctagcgccg gccgcggcga tgtccgccag cgccgcagcg caggccggcc aacgccgggc
     9841 cgcggtactg gccgaccgcg tcgtcccatt gctgttcgtg ctgctgtgga gcagttcctt
     9901 catcgcggca cgcgttggct tgcggcagat gacgcctctg ctgttcgtcg cactgcgcat
     9961 ggtgctctgc gccgtggtgc tggtctcggt gatgctggtg ctacggcggt cctggtcggt
    10021 gctgcgcggc tgggtgtggt tccattgcgc ggtcgccggc gtgctggcga acggcgtgct
    10081 gctgatgacc gcccacgtgg cgatggtgca cacacaggcc gcgccgatcg cgctgatcca
    10141 gacgctcaat ccgctgttga ccgcggtgct tgcctggccg ttgctgggcg aacgcctgcg
    10201 gccgcagcaa tggattggct tgctgctggg cttgaccggg gtggtgctga tcctgggcct
    10261 tgccgcgctg cacagccgga cccagttcgg cgggctgctg ctgaccgccg gcggcgtgct
    10321 ggggctggtc gcgggcacgc tgtatttcgg ccgcttctgc cgcggcgtgc cactgctgga
    10381 agggaccacg gtccagttca ccgcctgtgc ggtggcgtgc gtggcctgca tggcgatctt
    10441 cgaaacgccg cacgccacct ggacccccgg cgccgtcgcg gcgatcgcct ggaacgccgc
    10501 cttcgtctcg ctcggcggca tggtgctgta tttcctgatg ctgaaacgcg gcgcggcggc
    10561 ccgcgcggcg gcgaacttct atctggtccc gggaactgcc gcggtgctgg cctgggtcct
    10621 gctgggtgag gcgctgtccg cgctgaccat cctgggcctg gtcatctcct cggtcggatg
    10681 ctggctggtg aaccggcggt agaaaaaggc gtaagccttg ctgcaccgtg gagcgcccgt
    10741 gttcgcagcg ccgccgctca cttcacggca cccctgcaaa tcgcgatttc gcgctccgcg
    10801 catcacgcgt tttgctgccc gcggaatgcc ggccgggcgg tcgcctccgc caatggatgt
    10861 acaaggcggg cagcaggttc gtgctcagca tcgcgctccc cacgatgccg ccgaggatca
    10921 ccaacgccag cccctgctcc ggcgccgcgc ctttgcccca gcccagcgcg gtcggcagca
    10981 tgcccagcac cgcggtcagc gtggtgagca ggatcgggcg gaaccgcacc cgcaccgctt
    11041 cgcgcgtggc ggcttccggt gacaggcccg cggcctcgtt gcggcgggca cggtccagca
    11101 gcac
//
LOCUS       DASSZH010000594         2876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_201922, whole genome shotgun sequence.
ACCESSION   DASSZH010000594 DASSZH010000000
VERSION     DASSZH010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201922"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VFN42_12895"
     CDS             <1..338
                     /locus_tag="VFN42_12895"
                     /inference="COORDINATES: protein motif:HMM:NF014144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar FliJ family protein"
                     /protein_id="HET8997558.1"
                     /translation="AIARETATACSLEGGDQAVQAFAAWLQRIRPSAREAAEAVERAV
                     LRTAEARAVVAAARAAAEAAETLVARRAAEREMDRSRAEQRALDEVAGAGRNARTERQ
                     RPGLPAGKE"
     gene            576..941
                     /locus_tag="VFN42_12900"
     CDS             576..941
                     /locus_tag="VFN42_12900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8997559.1"
                     /translation="MAGRSHILVVEDDPLVCEVLSAALDEVYATTNVGTSGAALARLR
                     DGGIDLMLLDCTLPDGVDVRLIPEADGSGVPVILMSGDPARVEKLAEQPRPFVVKPFT
                     ITTLLSTVEGVIRARGAAA"
     gene            1480..>2876
                     /gene="fusA"
                     /locus_tag="VFN42_12905"
     CDS             1480..>2876
                     /gene="fusA"
                     /locus_tag="VFN42_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET8997560.1"
                     /translation="MPVTPLERIRNIGITAHIDAGKTTTTERILYYTGMSHRIGEVHD
                     GNTTTDYMEQERERGITITSAAVTCEWKDHRINIIDTPGHIDFNIEVNRSLRVLDGAV
                     FIIEGVAGVQPQSETNWRLADRYNVPRIIFINKLDRTGADFYRAFATLKEKLDIVALP
                     LQLPIGIEDKFLGVVDLVEMHAVIWDGSELGASFHIEPIPADLEEQAKEYRQLLLDTA
                     LSVDDAAMEEYFDKGDVAIETMKRCIKAGTISGAFRPVLCGTAFKNKGVQPLLDAVLD
                     YLPSPLDVPGISVAAAEGEEDGEFSDRRRIKADPDAPFAGLAFKIINDKYGTLTFVRV
                     YSGTLKSGDMVLNTTKEHRERIGRMFQMHADKRAEIKEVSAGDIAAFVGLKDTGTGDT
                     LASQQDPVVLERMAFPVPVIDISVEPRTKEAVEKMTIGLQKLAAEDPSLRLRTDQETG
                     QTILSGMGELHLEII"
BASE COUNT          510 a          907 c         1009 g          450 t
ORIGIN      
        1 tggccatcgc ccgggaaacc gcgacagcat gcagcctgga gggtggcgat caggcggtgc
       61 aggcctttgc cgcgtggctg cagcgcatcc gcccctcggc gcgtgaggct gccgaagccg
      121 ttgaacgtgc cgtgctgcgc acggcggaag cgcgtgccgt cgtcgccgcc gcaagggccg
      181 cggcggaagc ggccgagacc ttggtggcgc ggcgggccgc cgagcgggag atggaccggt
      241 cccgggcgga acagcgcgcg ctggacgagg tggctggggc cggccggaac gcccggaccg
      301 agcgccagag gccgggtctt ccggccggca aggagtagcg gaaggcggaa aagggccggg
      361 gttgtttcgg cctgggttcc ccgtcgaatc tgctctattg tgggatgccc ggcatatgaa
      421 tggcgggatg tgggggtggg gccccgtgct cctcgcgaca aattgcacga gagcgtactg
      481 gtccccactt ttgtgccgct ttgcggcctt gtaatgctct cggcattcac gccgcccgtg
      541 atgccgggat gcgaccgaat tgaggcaagt cgagaatggc tggcagatcg catatcctgg
      601 tggtggaaga tgatccgctc gtgtgtgagg tgctcagcgc cgcactggac gaggtttatg
      661 cgacaaccaa cgtcggaacc tccggcgcgg cgttggcgcg gctgcgggat ggcgggatcg
      721 acctgatgct gctggactgc accttgccgg acggcgtcga tgtccggctg atcccggaag
      781 ccgatgggtc tggcgtgccg gtgatcctga tgtcaggcga cccggcacgg gtggaaaagc
      841 tggcggagca gccgcggccc ttcgtggtga agcccttcac catcacgacg ctgctctcca
      901 cggtggaggg tgtgatccgg gcgcgcggcg cagcggccta gggcggctac accgcctccg
      961 ctcggaagcc cccggagggg gcggcgcagc agcggcaacc gccgggatgc gcccggacgg
     1021 gcgccatcgg catgctgggt tccgcgccaa cgccgccgcc gagggccggc gcgctcagga
     1081 acacccgcgg cgccgggtcg ccgcattccg ggcagggctg cggtgccgcg aattccgcca
     1141 tggggcggga ggcactgaac ggtccgcaac gctggcagcc ataatcataa ttcggcatcg
     1201 ggtcagcttc tccgcaggcg atacacgctc cagagtggca ttgccggaca gagcccggca
     1261 agcaccgtca aagcgctccc ggccctgatg catggccggc attacgcact gcggtgatcc
     1321 ccgttgcatt ccggacttgc aaaaccgcgg ccggtgacgc atctgacgct cggaaatagc
     1381 cggccgcctg ccggccggtc tgacgtgggc gcgcggggac ggggtcccct ggatccggac
     1441 aggatcacgc gccccgttgc ttcattggag gttttactcg tgcccgtcac gccgctcgaa
     1501 cggatccgca atatcggcat caccgcgcat atcgacgcgg gcaagaccac cacgaccgag
     1561 cgtatcctgt attatactgg tatgtcgcac aggatcggcg aggtgcacga cggcaacacc
     1621 acgaccgatt acatggagca ggagcgggag cggggcatca ccatcacctc cgccgcggtg
     1681 acgtgcgagt ggaaggacca ccgcatcaac atcatcgaca cgcccggcca catcgacttc
     1741 aacatcgagg tgaaccgcag cctgcgcgtg ctggacggcg cggtgttcat catcgagggc
     1801 gtcgccggcg tgcagccgca gtcggagacc aactggcggc tggccgaccg ctacaacgtc
     1861 ccgcgcatca tcttcatcaa caagctggac cgcaccggcg ccgatttcta ccgcgccttc
     1921 gccacgctga aggagaagct ggacatcgtc gcgctgccgc tgcagttgcc gatcggcatc
     1981 gaggacaagt tcctcggcgt ggtcgatctg gtggaaatgc acgcggtgat ctgggacggc
     2041 agcgaattgg gcgccagctt ccacatcgag ccgattccgg ccgacctgga agagcaggcg
     2101 aaggaatacc gccaactgct gctcgacacc gcgctgtcgg tcgatgacgc cgcgatggag
     2161 gagtatttcg acaaaggcga cgtcgccatc gagacgatga agcgctgcat caaggcgggc
     2221 accatttccg gcgccttccg tccggtgctg tgtggtaccg cgttcaagaa caagggcgtg
     2281 cagccgctgc tcgacgcggt gctggattat ctgccgagcc cgctggacgt gccggggatt
     2341 tccgtggcgg cggccgaggg cgaggaggat ggcgagttca gcgaccgccg ccgaatcaag
     2401 gccgatcccg acgcgccctt cgccggactg gcgttcaaga tcatcaacga caagtacggc
     2461 acgctgacct tcgtgcgggt gtattccggc acgctgaagt ccggcgacat ggtgctgaac
     2521 accaccaagg agcaccgtga gcgcatcggc cgcatgttcc agatgcacgc cgacaagcgg
     2581 gcggagatca aggaagtgtc ggccggtgat atcgccgctt tcgtcgggct gaaggacacc
     2641 ggcacgggcg acaccttggc aagccagcag gatcccgtgg tgctggagcg catggccttc
     2701 ccggtgccgg tgatcgacat ttcggtagag ccgcgcacca aggaagcggt ggagaagatg
     2761 accatcggcc tgcagaagct ggcggcggaa gacccgtcgc tgcggctgcg caccgaccag
     2821 gaaaccggcc agaccatcct gtccggcatg ggtgagctgc acctggaaat catcat
//
LOCUS       DASSZH010000595         3741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1411539, whole genome shotgun sequence.
ACCESSION   DASSZH010000595 DASSZH010000000
VERSION     DASSZH010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411539"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VFN42_12910"
     CDS             <1..239
                     /locus_tag="VFN42_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter C-terminal domain-containing
                     protein"
                     /protein_id="HET8997561.1"
                     /translation="RRMSFKDRHALETLPARIAALQDQIAKVQVLLADPDLYARDAAR
                     FAKATEALTSAQQALAAAEEQWLELEMLREELEG"
     gene            245..736
                     /locus_tag="VFN42_12915"
     CDS             245..736
                     /locus_tag="VFN42_12915"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET8997562.1"
                     /translation="MKTLLIVFHSRTGGTRQMAEAARRGAAAETGVRTRLLPAEEASG
                     ADVVAAEGYIFATPENLAAMAGRMKDFFDRTYYDALDRINGRPYALLVCAGSDGSGAV
                     RQVRRIVTGWRLREVAEPLIVCTHAQTPARILAAKAIGPAELERCEEIGSTLAAGLSA
                     GIF"
     gene            825..1574
                     /locus_tag="VFN42_12920"
     CDS             825..1574
                     /locus_tag="VFN42_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011253201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET8997563.1"
                     /translation="MAGHSQFKNIMHRKGAQDARRGRLFARLIREITVAARHGLPDPA
                     SNPRLRAAVVAARQANMPKDTVDRAIKKATGAGAGDDYVEVRYEGYGPAGVAVIVEAL
                     TDNRNRTASDVRSAFAKYGGALGETNSVSFLFNRLGVIRYPASVGSADDMLEAAIEAG
                     ADNVESDAEAHEVTCAVDDFFAVRDALEARFGEPESARLDWRPTTSVTLDEDKAASLM
                     KLLDALDENDDVQNVYANFDIPEAVMARLSA"
     gene            1620..3104
                     /locus_tag="VFN42_12925"
     CDS             1620..3104
                     /locus_tag="VFN42_12925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET8997564.1"
                     /translation="MTAGTAAREPARATILALLAGFVAIWTVYFTLTEAPVPIKHDML
                     EAYAWGQEFQLGYHQHPPFWAWICGLWFLVFPSTGWAFSLLSSVNAAIGVGGAWLLIG
                     DFARGSKRMAAWALLLLTPLYTVYAYKYDANIIFLSLWPWTLHFFVRSLRSRGLRDTL
                     LLGLFAGFALLSKYYALILLATCFLAALQSPQRRAWFASPAPYVAAAVTVLLCVPHVW
                     WLLNNRAPPVHYLESVSGRSWHHVWTHLGKTLGDAVAMNLGPAAVIALAFRLSRQRFA
                     LVTRGSADFPMLVTLVLAPLVLTLAGALVLRTTVTSEMTLGIFPLLPLLAIEISGLRD
                     VDKLSRMAVGIAAVANVGALLLSPAIMLSRTFLSPAAMKQAPLQEVAVAATRLWHART
                     GLKLRYVAGTAWYENATAFYSPDHPHVFEYFDYAMNLWVTPEDLARHGLLSICAADDK
                     SCLARTAHFVTPATTSTRLTLSHRFLGHVARPVAYVVTIIPPQR"
     gene            3183..3695
                     /gene="ruvC"
                     /locus_tag="VFN42_12930"
     CDS             3183..3695
                     /gene="ruvC"
                     /locus_tag="VFN42_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HET8997565.1"
                     /translation="MLRLLGIDPGLRFTGWGLIEADGNRLRHLADGVIATDGEAAVPD
                     RLKALHDALAALLAEYRPHEAAVEETYVNRNGAATLKLGYARGVALLAPALAGVPVTE
                     YAAKTVKMAVVGTGGAEKQQVQMMVRRLLPGATVRRAYAADALAVAICHAHHRSSRRA
                     WSVEAAQQRV"
BASE COUNT          562 a         1278 c         1289 g          612 t
ORIGIN      
        1 cgcggcgcat gagcttcaag gaccggcacg cgctggagac gctccccgcc cgcatcgccg
       61 cgttgcagga tcagatcgcc aaggtccagg tcctgctggc ggacccggat ctgtacgcgc
      121 gcgatgccgc tcgtttcgcc aaggcgaccg aggcgctgac ctccgcgcag caggcgctgg
      181 ccgcagccga ggagcagtgg ctggaactgg agatgctgcg ggaggaactg gagggctagg
      241 ccgcgtgaag accctgctca tcgtcttcca ctcccgcacc ggcggcacgc gccagatggc
      301 cgaagccgcg cggcgcggcg cggcggcaga gaccggcgtg cgcacgcggc tgctgccggc
      361 ggaggaggct tccggggcgg acgtggtggc agcggaaggc tacatcttcg ccacgccgga
      421 gaacctggcg gcgatggccg ggcggatgaa ggacttcttc gaccggactt attatgacgc
      481 gctggaccgg atcaacgggc ggccctacgc gctgctggtc tgcgccggca gcgacggcag
      541 cggcgcggtg cggcaggtcc ggcgcattgt cacgggctgg cgcctgcggg aagtcgccga
      601 accgttgatc gtctgcacgc acgcccagac gcccgcgcgc atcctggcgg cgaaggcgat
      661 cggaccagcc gagctggagc gttgcgagga aatcggttcg accctggctg cggggctctc
      721 ggccggcatc ttctagccgg caaaccgccc gcggcctgtc gcatccgccg gcgtgtcggc
      781 tataaccgcc ccacaccaga taactgacaa ctgatccctg acgcatggcc ggccattccc
      841 agttcaagaa catcatgcac cgcaagggcg cccaggacgc gcggcgaggc cgcctgttcg
      901 cccggttgat ccgcgaaatc accgtcgccg cccggcacgg cctgcccgac ccggcgtcga
      961 acccgcggtt gcgcgccgcc gtggtcgccg cgcgccaggc caacatgccg aaggacacgg
     1021 tggaccgcgc catcaagaag gccaccggcg ccggtgccgg cgacgactat gtggaggtgc
     1081 gttacgaggg ctacggcccc gccggcgtcg cggtgatcgt cgaggcgctg accgacaacc
     1141 gcaaccgcac cgcctccgac gtgcgttccg ccttcgccaa gtacggcggc gcgctgggcg
     1201 agaccaattc ggtcagcttc ctgttcaacc ggctgggcgt gatccgctac ccggcctcgg
     1261 tcggcagtgc cgatgacatg ctggaagctg cgatcgaagc cggggcggac aacgtggaaa
     1321 gcgacgccga agcgcatgag gtgacctgcg cggtggacga tttcttcgct gtccgcgacg
     1381 cgctggaagc tcgcttcggc gagccggaga gcgcgcgcct ggattggcgc ccgaccacct
     1441 cggtgaccct ggatgaggac aaggccgcgt cactgatgaa gctgctcgac gcgctggacg
     1501 agaacgacga cgtgcagaac gtctatgcca atttcgacat cccggaagca gtcatggcgc
     1561 ggctgtccgc gtagatgcgc atgccgtggt ggcgggcagc gccaccgggc gccggcagca
     1621 tgaccgcggg gacggcggcg cgcgagccgg cccgcgccac catcctggcg ctgctggccg
     1681 gcttcgtcgc gatctggaca gtgtatttca cgctgaccga agcgcccgtc ccgatcaagc
     1741 acgacatgct ggaagcctat gcctggggcc aggaattcca gctcggctac catcagcatc
     1801 cgccgttctg ggcctggatc tgcggtctgt ggttcctggt cttcccaagc accggctggg
     1861 ccttttcgct gctcagcagc gtcaatgcgg cgatcggcgt gggtggcgcg tggctgctga
     1921 tcggcgactt cgcgcgaggc agcaaacgca tggcggcctg ggcgctgctg ctgctgactc
     1981 cgctgtacac ggtctatgcc tataaatacg atgccaacat catcttcctc tcgctttggc
     2041 cgtggacgct gcacttcttc gtcaggtcgc tgcgcagccg aggcctgcgc gacacgctgt
     2101 tgctgggcct gtttgccggc ttcgcgctgc tgtccaaata ttatgcgctg atcctgctgg
     2161 cgacctgctt tctcgcggct ttgcaaagcc cgcagcggcg cgcctggttc gcatcgccgg
     2221 cgccctatgt cgcggccgcg gtcaccgtcc tgctctgtgt gccgcatgtc tggtggctgc
     2281 tgaacaaccg cgcgccgccg gtgcactacc tggagagcgt ctccgggcgt agctggcacc
     2341 atgtatggac ccatctcggc aagaccctgg gcgatgcggt ggccatgaat ctcgggccag
     2401 cggcggtcat cgccctggcc ttccggctct cgcggcaacg cttcgccctg gtcacccgcg
     2461 gcagcgccga ttttcccatg ctggtcacgc tggtgctggc accgctggtg ctgacgctcg
     2521 ccggtgctct ggtgctgcgc accaccgtca catcggaaat gacgctgggg atctttccgc
     2581 tgttgccgct gctggccatc gagatcagcg gcctgcgcga cgtggacaag ttgtcccgca
     2641 tggcggtcgg tatcgccgcg gtggcgaatg ttggcgcgct gctgctgtcc ccggcgatca
     2701 tgctgtcccg caccttcctc tcgccggccg ccatgaagca ggcgccgttg caggaggtgg
     2761 cggtcgccgc gacgcggttg tggcacgcac gcaccgggct gaagctgcgc tatgtcgcag
     2821 gcacggcttg gtacgagaac gccaccgcat tctacagccc cgaccacccg cacgtgttcg
     2881 agtatttcga ttacgccatg aacctgtggg ttacgcccga ggacctggcc cggcacggac
     2941 tgctttccat ctgcgcagcg gacgacaaaa gctgcctggc gaggaccgcg cacttcgtga
     3001 caccggcaac caccagcacc cggctgacac tctcgcaccg cttcctgggc catgtggcgc
     3061 ggccggtggc ctacgtggtg acgatcatcc cgccgcagcg ctgagccagg cggcgctggc
     3121 gtgcggagcg gttttcatgc gattcccgct gcctcgcggc acctcgggga cggattcgcg
     3181 ccatgcttcg cctgctcggc atcgatcccg gcctgcgctt caccggatgg ggcctcatcg
     3241 aggcggacgg caaccgcctg cgccacctgg ccgacggcgt gattgccacc gacggcgagg
     3301 ccgcggtacc ggaccggctg aaggcactgc acgacgcgtt ggccgcgctg ctggccgaat
     3361 atcgcccgca tgaggcggcg gtcgaggaga cctatgtcaa ccgcaacggt gccgccacgc
     3421 tgaagctggg ttacgcgcgc ggcgtggcct tgctggctcc ggctttggcc ggcgtgccgg
     3481 tgacggaata cgccgccaag acggtgaaga tggccgtggt gggcaccggg ggggcggaga
     3541 agcaacaggt gcagatgatg gtgcgccgcc tgctgcccgg cgccaccgtc cgccgcgcct
     3601 atgcggcgga cgccctggcg gtggcgatct gccacgcgca tcaccggtcg tcgcgccgag
     3661 cctggtcggt cgaagccgcg cagcagagag tttgaaagct gagcacggag cacaatgctc
     3721 cccctcccct tgcgggaggg g
//
LOCUS       DASSZH010000596         3986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_901021, whole genome shotgun sequence.
ACCESSION   DASSZH010000596 DASSZH010000000
VERSION     DASSZH010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901021"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(220..642)
                     /locus_tag="VFN42_12935"
     CDS             complement(220..642)
                     /locus_tag="VFN42_12935"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HET8997566.1"
                     /translation="MALKASQLKVGDTHSERVVSNLTRTQIVMYCGASGDYNPLHADE
                     VYVTKAAGYPSVFAHGMLTMGMTGKMLTNYVGDGRLTRFGVRFTNQVWPGDDLDARAT
                     VTALREEGGQHFVDLALSTTNQDGKEVLSGSATARIDA"
     gene            complement(653..1135)
                     /locus_tag="VFN42_12940"
     CDS             complement(653..1135)
                     /locus_tag="VFN42_12940"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HET8997567.1"
                     /translation="MAKITFPVEASHVMMFARAVGDTNPVYHDAEAARQSEVGGIIAP
                     PTFPQAVAQFDPDYHLRWRPDQPWFGSGKTPSGVEGKPSSSGGLHAEQHFEYFRPLRP
                     GDVLSVETKPGETWVKESKRAGKLTFRERITEYRDQKGELVVRARSVGVTTERPVDQG
                     "
     gene            1208..2026
                     /locus_tag="VFN42_12945"
     CDS             1208..2026
                     /locus_tag="VFN42_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET8997568.1"
                     /translation="MKRPLRRPLARTRSALASAEIPTWNGPRAVPDRADALRFVAAPG
                     RLPDGRRVYAIGDVHGSLDQLRALHAAVTEDLHARPVPSAVLVHLGDYIDHGADSAGV
                     LSLLCAGTDLPDLRVVNLLGEHERMLLDSLDGDRAAATDWLWSGGKATLASWGLDPDL
                     PREQWRTEIPAEHVAFLRGLALSHREGEYLFVHAGIRPGVPLARQTRDDLLGIRQPFL
                     WSEQDFGFVVVHGHSANPAPVTLANRIGLDTGAGLGGKLSCAVLEADRLALLAV"
     gene            complement(2134..2829)
                     /locus_tag="VFN42_12950"
     CDS             complement(2134..2829)
                     /locus_tag="VFN42_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006557423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET8997569.1"
                     /translation="MAIAASGFQFNGGEIMFKRQKPESAPLRKPDSLSSSTLSSLAAG
                     ETMARSSGVPAQGPGSSALPRPSGVAFPAKVSLHDADPIPALPSILPPIRGVARDAEL
                     AASRRETSQRRTLVVGRGISVQGTIQDAERLVVEGTVEASMVHAAELSVSAGGMFKGG
                     AEVEDAEVAGTIDGSLTVRGRLTVRATGKVLGTAHCHKLQVEDGGQVNGRVEMLAEAV
                     RSEREPAVMEAAD"
     gene            complement(2836..>3986)
                     /locus_tag="VFN42_12955"
     CDS             complement(2836..>3986)
                     /locus_tag="VFN42_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997570.1"
                     /translation="DYLDPPVRCEVNTLRSSAGNVHSIVRVQSHGAIPVCIARDGPVV
                     AGEKLVERGAFYVRKYGPAVHGRHISMPRPESGRVDSPQDWTVLIRRCVRQDRESLLA
                     MIDAAVEGRRPDPPLEQRLRAWHDAARAGFLALVPRSPAAESLKRRHFALSYGFELID
                     RRMLERGQLAELLRLAAFEQRTMFRDVLNMFDPPYRRTVQARFVSDAATGDEEAEFLE
                     VGWLRDRLPSETADFWRVSPSGLATILRDYAEDRSAENQARGMQPGRWFSPNLLAQEV
                     AELVCHARLMTRTFGNVRRVCFRCEWWGLAGRELFDPGATWISRGPCGDDHRVVTLHM
                     PAAGLAEGWIDVVAQLIAPVLRNFEPELQLTGDWVRSQVERWQSSGTS"
BASE COUNT          632 a         1423 c         1272 g          659 t
ORIGIN      
        1 attctgtcca tgttggccgc acagatcttc gatccgcgcg tcatgtggga cgccgccgag
       61 gccaaagcac ccgcggcaga accgcatggt cacccgctgc gcatttcgcc ttgacccttc
      121 actggccgga gcaaacggat ggcagcgcgg tgacgcaacc agaaggtggt atgcgtcacc
      181 gtcgctcagg ctcgacaggt cagacgcccg tcaaaaccct caagcatcga tccgtgcggt
      241 cgccgaaccg ctgagcactt ccttgccatc ctggttggtg gtggacagtg ccaggtcaac
      301 gaagtgctgg ccgccttcct cgcgcaacgc ggtcacggtc gccctggcgt cgaggtcatc
      361 gcccggccag acctggttgg tgaaccgcac gccgaacctg gtcagccgtc cgtcgccgac
      421 atagttcgtc agcatcttgc cggtcatccc catcgtcagc atgccgtgtg cgaagaccga
      481 gggatatcca gcggccttgg tcacgtacac ctcgtccgca tgcagcggat tgtagtcacc
      541 cgacgcgcca cagtacatca cgatctgcgt ccgggtcagg ttgctcacta cgcgctcgct
      601 gtgcgtgtcg ccgaccttga gctggcttgc cttgagtgcc attgccgcct cctcagccct
      661 ggtccacggg acgctcggtg gtgacgccga cgctgcgcgc ccgcaccacc agttcgccct
      721 tctggtcgcg atattcggtg atccgctcgc ggaaggtcag cttgcccgcg cgtttgctct
      781 cctttaccca ggtctcgccg ggcttcgtct cgacgctcag cacgtcaccc gggcgcagcg
      841 gccgaaaata ttcgaaatgc tgctccgcgt gcagcccgcc gctggaactc ggcttgccct
      901 ccacgccgga cggcgtcttg ccggaaccga accacggctg gtctggccgc cagcgcaggt
      961 gatagtccgg atcgaactgc gccacggcct gcgggaacgt tggtggcgcg atgatgccgc
     1021 ccacctcgga ttgccttgct gcctcggcat cgtgatacac cggattggtg tcgccgaccg
     1081 cgcgggcgaa catcatgacg tggctggctt cgacggggaa ggtgatcttg gccaacggtt
     1141 tcctccgctc cttgggttgc ggcaagccta gcaggacagc ggcattacgg aagaggggca
     1201 ggccagcgtg aagcgaccct tgcggcgtcc gctggcgcgc acccgcagcg cgctggcatc
     1261 ggccgagatc cccacctgga acggtccgcg cgccgtgccc gaccgcgccg acgcgctgcg
     1321 tttcgttgcc gcgcccggac gcctgccgga cgggcggcgg gtgtatgcga tcggcgacgt
     1381 gcatggcagc ctggatcagc tccgcgcgct gcacgcggcc gtcaccgagg atctgcacgc
     1441 caggccggtg ccctctgccg tgctggtgca tctgggcgac tacatcgacc atggtgcgga
     1501 cagtgccggc gtgctgtcgc tgctgtgtgc cggcaccgat ctgcctgatc tgcgcgtggt
     1561 caacctgctc ggcgagcacg aacgcatgct gctcgactcg ctggacggcg accgggcggc
     1621 cgcgaccgac tggctctggt cgggcggcaa ggccacgctg gcgagttggg gcctggatcc
     1681 ggatctgcca cgcgagcaat ggcgcacgga aattccggcc gagcatgtcg cattcctgcg
     1741 tggattggca ttgagccacc gggaaggcga gtatctgttc gtgcatgccg gcatccgccc
     1801 cggcgtgccg ctggcgcgcc agacgcgcga cgacctgttg ggcatccgcc agccgttcct
     1861 gtggagcgag caggatttcg gttttgtcgt ggtgcacgga cacagcgcca atcccgcgcc
     1921 ggtcacactg gcaaaccgca tcgggctgga taccggcgcc ggcctcggcg gcaagctgag
     1981 ctgcgcggtg ctggaggcag accgcctggc gctgcttgcc gtgtgactgg gagaccgagg
     2041 cacatatgcc ggtggcggaa cgcacaccag cacgtgtcca atttttcgcg cgaaccgaac
     2101 cggcctgcgc gccgccaatt cgcgccggca gcgctagtcc gccgcttcca tcaccgccgg
     2161 ttcacgctcc gaccggaccg cctcggcaag catttcgaca cgcccgttga cctgcccgcc
     2221 atcctcgacc tgaagcttgt ggcaatgcgc ggtgcccagc accttcccgg tggcccgcac
     2281 cgtcagcctg ccgcgaacgg tcagggagcc gtcgatcgtg ccggcaacct ccgcgtcctc
     2341 gacctccgca ccgcccttga acatgccccc ggccgagacg gacaattcgg cggcatgcac
     2401 catgcttgcc tcgacggtgc cttccaccac gagacgttca gcatcctgga tggtgccctg
     2461 cacgctgatg ccccgcccga ccaccaaggt ccgccgctgc gacgtttcgc ggcgggacgc
     2521 cgcaagctcg gcatcacgcg ccacgccgcg gatcggcggc aatatgcttg gcagagccgg
     2581 gatcgggtcc gcgtcgtgca gcgaaacctt ggctgggaag gcaacgccgg acggccgcgg
     2641 cagcgcgctg gagccaggcc cctgtgccgg cacaccggac gagcgcgcca tggtctcccc
     2701 cgccgcaagc gatgacaggg tggagctgga cagggagtcc ggcttgcgaa gtggggcgct
     2761 ctcgggcttc tggcgcttga acatgatctc tcctccgttg aactgaaaac ctgacgcggc
     2821 gatcgccacg cgctgtcacg atgtcccgct gctctgccag cgttccacct gcgagcgcac
     2881 ccagtcgcca gtgagctgca gctccggctc gaaattgcgc agcaccgggg cgatcaactg
     2941 cgccacgacg tcaatccagc cttcggccag tcccgctgcg ggcatatgca atgtcaccac
     3001 gcgatggtcg tcgccgcagg gtccgcggga aatccaggtt gcccccggat cgaacagctc
     3061 acgccccgcc aatccccacc actcgcagcg gaaacagacc cgccgcacat tgccgaaggt
     3121 ccgcgtcatg agacgcgcgt ggcagaccag ttccgccact tcctgcgcca acaggttcgg
     3181 actgaaccag cgtccgggct gcatgccgcg cgcctggttc tccgcactgc gatcttccgc
     3241 gtagtcgcgc aggatggtgg ccaggccgct gggggagacg cgccagaaat ccgccgtctc
     3301 cgacgggagc cggtcgcgca gccaacccac ttccaggaac tccgcctcct cgtcgccggt
     3361 ggcggcgtca ctgacgaagc gcgcctgcac cgtacggcga tagggcggat cgaacatgtt
     3421 cagcacatcg cggaacatcg ttctctgctc gaacgccgcc agccgcagca actccgccag
     3481 ctgcccgcgt tccagcatgc gccggtcaat cagctcgaaa ccatacgaca gcgcgaaatg
     3541 ccgccgcttc aacgactcgg ctgctgggct gcgcggcacc aatgcgagga atccggcacg
     3601 cgcggcgtcg tgccaggcgc ggagccgctg ctccagcggc ggatcgggcc tgcgcccttc
     3661 caccgcggca tcgatcatcg ccagcaggct ttcccggtcc tgccgcacgc agcggcggat
     3721 caggacggtc cagtcctgcg gcgagtcgac ccggccgctc tccggacgcg gcatgctgat
     3781 atggcggcca tgtacggccg gtccgtattt gcgaacatag aacgcgccgc gttccaccag
     3841 cttctcaccg gccaccaccg gcccgtcgcg tgcgatgcac accgggatgg ctccgtggct
     3901 ctgcacccgg acgatgctgt gcacattgcc ggccgacgaa cgcagcgtgt tcacctcgca
     3961 gcgcaccggc ggatcgagat agtccc
//
LOCUS       DASSZH010000597         2904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_730279, whole genome shotgun sequence.
ACCESSION   DASSZH010000597 DASSZH010000000
VERSION     DASSZH010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730279"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VFN42_12960"
     CDS             complement(<1..640)
                     /locus_tag="VFN42_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmI/RlmK family 23S rRNA methyltransferase"
                     /protein_id="HET8997571.1"
                     /translation="MEPDDATGGRPLLKLSPGQGRRLRAGAPWVFSNEIAMRPEYRRM
                     EPGTLVRLEGDDGVRLGTFMFNSHSLIAARLLDRDPAAAIDLDWMRRRLESAIALRTR
                     VCDTAFHRLVHAEADRLPGLVIDRYGDVAVLQANSAGMDRLTPLVVQALTDLLSLRAV
                     VARNDSASRRLEGLPESVALLHGSDAAAMVEEGGVRFPVDPLSGQKTGWFFDQ"
     gene            639..2111
                     /locus_tag="VFN42_12965"
     CDS             639..2111
                     /locus_tag="VFN42_12965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HET8997572.1"
                     /translation="MRRGRPRPPRQGKAARLKPFDKPPLPISLAMTSTPGHSELRHAG
                     ECRDPRVSSRERSRAWRAFARYHAVPPPGRSPLIAALRDGRWLHRNRIIAWGSMLLTA
                     ELLLVLVIAAWQHGLIGHFNAPVSSDFLSFYAAGKLALAGTPALAYDHAAHYLAEQQV
                     AGTGVPYQFFFYPPVFLLPCAVLALLPYALAYALFEAGTLALFLLAMRAILRPSGWGW
                     MLPVLAFPAVFWTIGLGQNAFLTAALFGGFTLLLDRRPVSAGLLLSAMCYKPHFGLLA
                     PVALAAGGRWRAFAAAAAGVVGLVGASLWLFGGDIWTAYLHGFAASGSVYETGRIQFA
                     GMVTPFGGMLLLGFGVWPAYAVQALATVSAAVLVGLVWRRSPDLSLRSAALLAGTLLA
                     VPLALLYDQMLWLVALGWLVHAGRRDGFLPWEKLVLLACYPLILLTWPIATGLHVPLG
                     PVESAALLLLVARRVWWHPVRQSGRQLEFGRSGSVATATP"
     gene            complement(2120..>2904)
                     /gene="mltG"
                     /locus_tag="VFN42_12970"
     CDS             complement(2120..>2904)
                     /gene="mltG"
                     /locus_tag="VFN42_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET8997573.1"
                     /translation="RAGPLHAAELAFPAHASLRQVLAVLRFAPPVQHSLTIPEGLTAQ
                     QIAALVNAAPAATGHVAPPREGSVLPQTYDYTYGTARSALLARAGAAMTRALADAWAD
                     RAADLPLATPRQALILASIVERETALPAERPHVAAVYLNRLRLGMRLQADPTVIYAAS
                     DGAGALGHTLTRSELDSNDPYNTYRHRGLPPGPICSPGIASIQAVLHPAASDDLYFVA
                     DGKGGHLFARTLAEQDRHVAAWRALEAARAADPPDKPATEKR"
BASE COUNT          367 a         1044 c         1004 g          489 t
ORIGIN      
        1 gctgatcgaa gaaccagccg gtcttctgcc cggacagcgg atcgaccgga aagcggacgc
       61 cgccttcctc caccatcgcg gccgcatccg acccatgcag caaggcgacc gactcgggca
      121 ggccttccag ccggcgcgac gccgaatcgt tgcgcgcgac cacggcacgc agcgacagca
      181 ggtccgtcag cgcctgcacc accagcggcg tcagccggtc catgcccgcg ctgttggcct
      241 gcagcaccgc cacatcacca tagcggtcga tcaccagccc gggcagccgg tccgcttcgg
      301 cgtgcaccag ccgatggaat gccgtgtcgc aaacccgggt ccgcagcgcg atggccgatt
      361 ccagccggcg ccgcatccaa tccaggtcga tggccgccgc cggatcgcgg tccagcagcc
      421 gtgccgcaat caggctgtgc gagttgaaca tgaaggtgcc caagcggacg ccgtcgtcgc
      481 cttccaggcg caccagcgtg ccaggttcca tgcggcggta ctccgggcgc atggcgatct
      541 cgttgctgaa cacccagggc gcgccagcgc gaagccggcg cccctgcccg ggtgacagtt
      601 tcaacagcgg ccggccgccg gttgcatcgt ctggttccat gcgccgaggc agaccacgcc
      661 cgccgcgcca gggcaaggcg gcacggctga aaccattcga caagccgccg ctgcctatat
      721 cgctcgcgat gacctcgact cccgggcatt ccgaactccg tcatgccggc gaatgccggg
      781 atccccgcgt ttcgtcgcgc gaacgaagtc gagcgtggcg ggccttcgcc cgctaccacg
      841 cggtgccgcc gcctggccgc tccccgctga tcgccgcgct gcgcgatggc cgctggctgc
      901 accgcaaccg catcatcgcc tggggatcga tgctgctgac cgccgagctg ctgttggtgc
      961 tggtgattgc ggcttggcag catgggctga tcggccattt caacgccccg gtctccagcg
     1021 atttcctcag cttctacgcg gccggcaagc tggccctcgc cggcactccg gcgctcgcct
     1081 acgaccacgc ggcgcattac ctggccgaac agcaggtcgc cggaaccggg gtgccgtacc
     1141 agttcttctt ttatcccccg gtcttcctgc tgccctgcgc ggtgctggcc ctgctgccct
     1201 atgccctggc ctacgcgttg ttcgaagccg gaaccctcgc tttgttcctg ctggcgatgc
     1261 gggcgatcct gcggccgagc ggctggggtt ggatgcttcc ggtgctggcc tttccggcgg
     1321 tgttctggac gatcgggctg ggccagaacg ccttcctgac cgccgcgctg ttcggcgggt
     1381 tcaccctgct gctcgaccgc cgcccggtct ccgccgggct gctgctgagc gccatgtgct
     1441 acaagccgca tttcgggctg ctggcgccgg tcgcgcttgc ggcgggcggg cggtggcgcg
     1501 ccttcgcggc agcggcagcc ggcgttgtcg ggttggtggg ggcgtcgctc tggctgttcg
     1561 gaggggacat ctggaccgcc tatcttcacg gcttcgccgc atcgggcagc gtgtatgaaa
     1621 ccggccgcat ccagttcgcc ggcatggtga ccccgttcgg cggcatgctg ctgctcggct
     1681 ttggcgtctg gcccgcttat gccgtgcagg ccctggccac cgtttcagcc gccgtgctgg
     1741 tcgggctggt ctggcggcgg agccccgatc tgtcgctccg cagcgcggcg ctgctggccg
     1801 gcacgctgct cgcggtgccg ctggcgctgc tctacgatca gatgttgtgg ctggtcgccc
     1861 tcggctggct ggtgcatgcc ggccggcgtg atggcttcct gccctgggag aagctggtac
     1921 tgctcgcatg ctacccgctc atcctgctca cctggccgat cgccaccggc ctgcatgtgc
     1981 cgctgggtcc ggtggaaagc gcggcgctgc tgctgctggt tgcgcggcgc gtctggtggc
     2041 atccggtccg gcagagcggc aggcagttgg aatttggccg atctggttcg gtcgccacgg
     2101 cgacgccctg atcgctgctt cagcgcttct ccgtggcggg tttgtcgggt ggatccgcgg
     2161 cacgcgccgc ctccagcgcc cgccacgcgg cgacatgccg gtcctgctcc gccagcgtgc
     2221 gggcgaacag gtgcccgcct ttgccgtccg ccacaaagta caggtcgtcg ctcgccgccg
     2281 gatgcagcac cgcctggatc gaagcgatgc ccggcgaaca gatcgggccg ggcggcaatc
     2341 cgcggtgccg ataggtgttg tacgggtcgt tcgagtccag ctcgctccgg gtcagcgtgt
     2401 ggcccagcgc ccctgccccg tcgctggccg cgtagatcac cgtgggatca gcctgcaacc
     2461 gcatgcccag ccgcaggcgg ttgagataga ccgcggcgac atgcgggcgc tcggccggca
     2521 gtgctgtctc gcgctcgaca atactggcca ggatcagcgc ctggcgcggg gtggccaggg
     2581 gcaggtcggc ggcccggtcg gcccaggcat ccgcgagcgc gcgggtcatc gccgccccgg
     2641 cgcgcgccag cagggcggag cgcgctgtgc cataggtata gtcataggtc tgcggcagca
     2701 cgctgccctc tcgcggcggc gccacatggc cggtggcggc tggcgcggca ttcaccaggg
     2761 ccgcgatctg ctgcgcggtc agcccttccg gaatggtcag cgaatgttgc accggcggcg
     2821 cgaagcgcag caccgccagg acctgccgca gcgaggcatg cgccgggaac gccagctccg
     2881 ccgcatgcag cgggccggcc cgcg
//
LOCUS       DASSZH010000598         5094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_764225, whole genome shotgun sequence.
ACCESSION   DASSZH010000598 DASSZH010000000
VERSION     DASSZH010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764225"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /locus_tag="VFN42_12975"
     CDS             complement(<1..1191)
                     /locus_tag="VFN42_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997574.1"
                     /translation="MPDTDHVRWDETAEADAFCRQPWEDDPAPDHDPLLHPAWDDLPD
                     EITPPAPPLRSRSAPTHRAADAHGLLAPLAAAQDALARLDASAEAAPDPIRIGLIARL
                     ALREAAGWLASQSAWVHPHDLALRIANLAGRFDTAAQIARPRTTLPGTISAIPGGWDD
                     PEDLAALARGELAAVRALALSRLLAALPRRHDPLATPETAAALLGPLGAGALEPDRFA
                     AWRAAHLPGRGRSGTSRAPALPPLLQAADAALAWMESGASDEPDALAALALAALLLTR
                     SGTPRIIPLPFWAAWPALGHPVGEGLPRLRPAVARDSAGMERVPWPAVFLTFVAEAAR
                     AGLRELGRLRAAAAAGAALGKGLDKRSRLPAAVTLVLAAPVVTARDLATRLGITPQAA
                     NRILG"
     gene            2033..3919
                     /locus_tag="VFN42_12980"
     CDS             2033..3919
                     /locus_tag="VFN42_12980"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET8997575.1"
                     /translation="MLDLFGKGKLSGTTHTCLGQEFCQMAVVRALQHEDDAVLSNHRN
                     HGHFLTYSGDFTGLVAEVMGRQDGVCGGRGGSQHMAWRHFHSNGVQAGMTGIAVGLAR
                     AREMSASPGIVAVIVGDGTLGEGLLYESMNLAAVWPAPVLFVVEHNGIAQTTDTADTI
                     GGDILARGAAFGLSTYRVSDADPELWHVAETAVADVRRTRRPAFLVIDTARLGPHSKG
                     DDLRPEADIAAIRARDPVARLGATLDPAERDRLDAAVGELVNRVANAAAASPEAHELV
                     AVDTVFRHAPQLPAVDPSAASSGQTVRAALNATLDALLTEDPRTILLGEDLHDPYGGA
                     FKVTAGLSQRYPGRVVSTPISEGGITGAAIGLALAGKRPIMEVMFGDFLTLCADQIYN
                     HAVKFPGLFPELSVPMVIRTPVGGRRGYGPTHSQSPENIFTAIPGLTVLYGSQRHDNG
                     ALLRNAVLHWPFPTLFLEHKLVYGATEDPGAYHPLPAHPADIGGAAFPTLQRRSLQAD
                     AVLLTYGGMVPLVEAVADQLAAEEELELDIIILAQLAPLPRHALLAALTAYDAIVIAE
                     ETHTGYGVGAEIIASLAEAGYRGSIRRVGTPPVPIPSARSLELAVLPDADCLAAAVLD
                     AIEA"
     gene            3940..5055
                     /locus_tag="VFN42_12985"
     CDS             3940..5055
                     /locus_tag="VFN42_12985"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HET8997576.1"
                     /translation="MSHPVYVPRVNNNDDVVKVVHIAVAPGDAVATGDLLMEVETEKA
                     VVEVAAERDGYVLAVLCESGQSIAVGAVAMWCGATPEEAAPQASSRPEATDSRPAGDM
                     TAKARMLLRRHGLAADLIPRSGPRLLATDIEAYLATQARSSAPKPASREPLTLPAPAE
                     IEPLSPAARGMLATVTWHRDQAAAAYLEIEYDPRPWDKLAADFMGERRLLLNPLLALM
                     AHRLAQLAPGMGANGTILEDGDHRVVRYRQVNLGFTVQAGEMLYLCVVAAAEAMTAAQ
                     FVTRLSDLQRRAMGHKLTPAEMRGATIGFTSMARWGVQRHQPILPPHTGAMIAHTAPA
                     PGGERAILGITYDHRLLSGFAAVRLLKKLVQPGNDGA"
BASE COUNT          824 a         1713 c         1750 g          807 t
ORIGIN      
        1 tcccaggatc cggtttgccg cctggggcgt gatccccagc cgggtggcga gatcccgcgc
       61 cgttaccaca ggtgccgcca gcactagcgt gaccgccgcc ggcaggcgtg agcgcttgtc
      121 gagccctttc cccagcgccg cgccggccgc cgccgcggcg cgcagccggc cgagttcccg
      181 cagcccggcg cgcgccgcct cggcgacgaa ggtgaggaag acggccggcc agggcacccg
      241 ctccatgccg gcgctgtctc gtgccacggc cgggcgcaac cgtggcaggc cctccccgac
      301 gggatggccg agcgccggcc atgcggccca gaagggcagg gggattatcc ttggtgtccc
      361 gcttcgggtg agcaggagcg cggcgagcgc cagggcagcg agcgcgtctg gctcatcgct
      421 cgcgccggat tccatccatg ccagggcggc gtcggcagcc tgcagcagcg gcggcagggc
      481 aggggcccgg gatgtcccgc tcctgccgcg gccaggcaga tgcgccgccc gccaggcggc
      541 gaagcggtcc ggctcgagcg cgccagcacc aagcggcccc agcaaggccg cggcagtctc
      601 tggcgtggcg agcggatcat gccggcgagg cagggcggcg agcaaccgcg acagggccag
      661 cgcccggacc gccgccaact cgccccgcgc cagggcggcg aggtcctccg ggtcgtccca
      721 accgcctggt atggcggaga tcgtgccggg cagggtagtg cgtggacggg caatctgcgc
      781 cgcggtgtcg aaccggccgg cgaggttggc gatgcgcaac gccaagtcgt gcggatgtac
      841 ccaggcgctt tgggaggcga gccagccggc ggcctcgcgt aacgccaggc gggcgatcag
      901 gccgatgcgg atagggtcgg gcgcggcttc cgcgctagcg tcgagccggg cgagggcatc
      961 ctgggcggcg gccagcggtg cgagcagtcc gtgggcgtcg gcagcgcgat gggtgggtgc
     1021 cgagcgggat cggaggggcg gggcaggggg ggtgatttcg tcgggcagat cgtcccaggc
     1081 tgggtgcagc agcggatcgt ggtccggggc ggggtcgtct tcccagggct ggcggcaaaa
     1141 ggcgtccgcc tcggcggtct catcccagcg cacgtggtcg gtatcgggca cagcggcgtt
     1201 tcccaatggc cttgagtttc cgcgtctggt ctagccgttt cgaaccgatt ccggccaaaa
     1261 gaattcgctg atcgggggct tctgtgagac cgcggcaggc ggcacgcagc gggaacggga
     1321 tggctgctga ttatgtccgt taacagtact ttgcgggcgg aatccagacc tcgctgcatg
     1381 ccgagggtgc gcggccggtg ctgcaaagcg ccgcagaggc ggactgccct agagacttgc
     1441 atgccagctg gcacgatctt ggctgccgat gtcaggagga agaccggaca ggatgccatc
     1501 agagtagtgt cagcggtttt gcctgcgccg gatccggggg cctgcggaag cgcgtccgcc
     1561 ggttcagagt ttatgcctca cccgggtagc attactaacg tatccaatca acggggtcat
     1621 tttaccgcct gcattgttgg gggaaacaag cgtgtatcga atcaaggaag catcgcccgt
     1681 tgcagacagg tggcgccatt gttgcgacga ggaaatccgt gaccggtcgc atcaccggca
     1741 acaagcggcc agatatcggt tgaccgcttc gcagcggtcc cgtttcccgc gaaatcccgc
     1801 aacatctcgt tgacctccca tggtttttga ccgaaaatgc aacatcagaa attccgttgc
     1861 gccgcaccag gctgaacaga tcagaggaat gagcatgcaa gcatcggcga tggccgcagt
     1921 ggccatcggc gtacctggcg ttggccggaa tccggatggc cggccaatca ttccgcttga
     1981 gccgaacgcc ctctatgcct acgccacatg cattcgcggc atcgagtccc tggtgttaga
     2041 cctcttcggc aaaggcaaac tctccggcac cacccatacc tgcctcggcc aggaattctg
     2101 ccagatggcc gtggtccgcg ccctgcagca tgaagatgat gcggtgctgt ccaatcaccg
     2161 caatcacggg catttcctga cctactccgg ggatttcacc ggcctggtcg ccgaagtgat
     2221 gggccgccag gatggcgtct gcggcggccg cggcggcagc cagcacatgg cctggcggca
     2281 tttccacagt aatggcgtgc aggccggcat gaccggcatt gccgtcgggc tggcacgtgc
     2341 ccgcgaaatg tccgcctcgc ccggcatcgt cgcggtcatc gtcggcgacg ggacgctcgg
     2401 cgagggcctg ctgtacgaaa gcatgaacct ggcggcggtc tggccggctc ccgtgctgtt
     2461 cgtggtggaa cacaatggca tcgcccagac caccgatacc gccgatacga tcggtggtga
     2521 tatcctggcc cgcggtgcgg cgttcggatt gtccacctac cgggtttccg atgccgatcc
     2581 ggaactgtgg catgtcgccg aaaccgccgt ggccgacgtg cgcaggacac ggcggcccgc
     2641 cttcctggtg atcgacaccg cccgcctcgg cccgcacagc aagggcgacg accttcgccc
     2701 cgaagcggac atcgccgcca tccgggcgcg cgacccggtc gcgcggcttg gcgcgacgct
     2761 cgatccggcc gaacgcgacc gcctggatgc agcggtcgga gagctggtga atcgggtggc
     2821 aaatgccgcc gccgcatccc ccgaggcaca tgagctcgtg gcggtggaca ccgtgttccg
     2881 gcacgccccg caacttcccg cggtggatcc gtcagccgct tcgtccggcc agacagtccg
     2941 ggcggcgctc aacgcgacac tcgatgccct gctgaccgaa gatccgcgca ccatcctgct
     3001 gggcgaggat ctgcacgacc cgtatggcgg tgccttcaag gtcaccgcgg gattgtcgca
     3061 gcgctacccc gggcgggtgg tttcaacgcc gatcagcgaa ggcggcataa ccggggccgc
     3121 catcggcctg gcgctggccg gcaagcgccc gatcatggag gtgatgttcg gcgatttcct
     3181 cacgctctgc gccgaccaga tctacaacca tgcggtgaaa ttccccgggc tgtttccgga
     3241 gctttcggtg ccgatggtca tccgtacccc ggtcggcggg cgccgcggtt acggcccgac
     3301 gcacagccag agcccggaga acatcttcac cgcaatccca ggcctgaccg tgctctatgg
     3361 cagccagcgg cacgacaacg gcgccctgct gcgcaacgcg gtgctgcatt ggccattccc
     3421 cacgctgttt ctggaacaca agctggtcta cggcgccacc gaggatccgg gcgcctacca
     3481 tccgctgccg gcgcatccgg ccgatatcgg cggcgccgct ttccccacat tgcaacggcg
     3541 cagcctgcag gccgatgcgg ttctgctgac ctatggcggc atggtgccgc tggtcgaggc
     3601 cgttgccgac cagctcgccg cggaagagga gctggaactc gacatcatca tcctggccca
     3661 gctcgccccg ttgccgcggc atgccctgct ggcggcgctg acggcgtatg atgccatcgt
     3721 catcgccgag gagacccata ccggctacgg cgtcggcgcc gaaatcatcg cctctctggc
     3781 cgaagcgggc tatcgcggca gcatccgccg cgtcggcacg ccgccggtgc ctatcccctc
     3841 cgcccgttcg ctcgaattgg cggtgcttcc ggatgccgat tgcctggccg cagccgttct
     3901 ggatgcgatc gaggcctgat ctcagttagt gcaggcagca tgtcccaccc cgtctacgtc
     3961 ccgcgagtca acaacaacga cgacgtcgtg aaggtggtgc atatcgccgt tgccccgggt
     4021 gatgccgtgg cgaccggtga tctgctcatg gaagtggaaa ccgaaaaggc cgtggtggag
     4081 gtcgccgcgg agcgcgacgg ctacgtcctc gccgtgctct gcgagtccgg gcagagcatt
     4141 gctgttggcg cggtggcgat gtggtgcggc gccacgccgg aggaggcagc accccaggcc
     4201 tcctcccgtc cggaggccac ggacagcaga cccgctggcg acatgacggc gaaggcgcgg
     4261 atgctgctgc ggcggcacgg actcgcagcc gatctcattc cgcgcagcgg gccgcgcctg
     4321 ttggccaccg acatcgaggc gtatctcgcc acgcaggcgc ggagcagcgc accgaagccg
     4381 gcgtccaggg aaccgctcac gctacccgcg ccggcggaga tcgagcctct gtctccggcc
     4441 gcccgcggca tgctggcgac ggtcacctgg caccgggacc aagccgcggc agcctatctg
     4501 gagatcgagt acgatccacg cccatgggac aagcttgctg ccgacttcat gggcgaacgc
     4561 cgcttgctgc tgaatccgct gctggcgctg atggctcacc ggctggcaca gctggcgccg
     4621 ggaatgggcg cgaacggcac catactggaa gacggcgatc accgggtggt gcgctatcgc
     4681 caagtcaacc tcggcttcac cgtgcaggcc ggcgagatgc tgtatctctg cgtggtggca
     4741 gccgccgagg ccatgacggc ggcgcaattt gtcacccgcc tgtccgacct gcaacgcaga
     4801 gccatgggcc acaagctgac tccggcagaa atgcgcggcg ccaccatcgg cttcaccagc
     4861 atggcgcggt ggggcgtaca gcggcatcag ccgatcctgc cgccgcatac cggcgcgatg
     4921 atcgcccaca ccgcccccgc accgggaggc gaacgggcga ttctcgggat cacctacgat
     4981 catcgcttgc tgtccggctt tgctgcggtg cggctgttga agaagctggt gcaacccggt
     5041 aatgatggcg cttgaggatc accatggcag atgatcgggc cgcaatcatc accg
//
LOCUS       DASSZH010000599         2244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1036774, whole genome shotgun sequence.
ACCESSION   DASSZH010000599 DASSZH010000000
VERSION     DASSZH010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036774"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2207)
                     /locus_tag="VFN42_12990"
     CDS             complement(<1..2207)
                     /locus_tag="VFN42_12990"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET8997577.1"
                     /translation="MDVQTEAPGFSAEQVETLVTRPVETAIGGVAGLQRTTSNSIAGL
                     SDIKVYFDLSTDPYRARQLVAERLAAAANALPTGTGPPRMTPLTSSMATVLVIGLTSP
                     SLSLMQLRSLATWTVRPRLLAVPGVAAVEIFGGDQRATQIRVHPDKLIKYRLDMGAVL
                     AAARQAVGVRGAGLLSTPNQDITLRTEAATQPADIAQAVVAWRDGGAITLGDVADVIE
                     APMPAVGGAAIMGQPGVVMNIDEQYGANTLEVTRGIEHALGDLRPSLDRAGAVLHGRL
                     FRPANFITVAVDNLRSSLVIGGLLVIAVLFLFLADLYAAAICCLAIPISLLAAVIVID
                     YFGITLNTMTLSGLAIALGEVVDDAVIGIENITRRLRENARATVPRPAALVVLEATFE
                     VRTAVVYATFAVILVFLPIVTLSGIAGRLFAPLGIGYIAAVLASLAVALTVTPALSLL
                     LLPRRTRQHEPRLMRWTRRRYEIMLHPIVRRPRLAIAAAVTLTTAGIALLPMFGQTFL
                     PELKEGHFIVHMSAIPGTSIAESLRMGARLTDQLEKLPIVRSVAQRAGRAELAAAGDT
                     HGPHQSEFEVDLGSLSSDAAQSAKADILRQLADLPGVNISANTFLAERVDETFSGYTT
                     PVAVNIFGNDLQVLARTAEQVAGVLRGLKGASGVQVQSPSDLPQVTVRLRPGALRHWG
                     LDAVPVLDVIRAAYEGLVVGQTYQGDRVFDVLVRLAPQAGANLSALGALPIRTP"
BASE COUNT          411 a          754 c          746 g          333 t
ORIGIN      
        1 ggtgtacgga tcggcaatgc tcccagggcc gaaagattcg ccccggcctg cggcgccagt
       61 cgcaccagca cgtcgaacac acgatcgccc tgataggtct ggccgaccac caggccttca
      121 taagccgctc ggatcacgtc cagtaccggc acggcgtcca gcccccaatg ccgcagggca
      181 ccgggccgca gccggaccgt cacctgcggc aggtccgacg gcgattgcac ctgcacaccg
      241 ctggcaccct tgagtccacg caacacgccg gccacctgct cggcggtccg ggccagcacc
      301 tgcaggtcat tgccaaaaat gttcacggcg accggcgtgg tatagcccga aaatgtttca
      361 tccacgcgct cggccaggaa cgtgttggcg gaaatgttca ccccgggcaa atctgcaagc
      421 tgccgcagga tatccgcctt ggcggattgt gctgcatcgg acgacagcga gcccagatcg
      481 acttcgaatt cgctctgatg cggcccatgc gtgtcgccgg ccgcggccag ttccgcccgg
      541 ccggcgcgct gcgccacgga gcggacgatt ggcaacttct caagctgatc cgtcaggcgt
      601 gcgcccatgc gcagcgactc agcgatcgag gtgccaggaa tcgcgctcat atgtacgatg
      661 aagtggcctt ccttcagctc cggcagaaag gtctggccga acatcggcag cagcgcgatg
      721 cccgcggtgg tgagcgtgac tgcggcggcg atggcaaggc gcggccgccg gacgatcgga
      781 tgcagcatga tttcatagcg ccggcgggtc catcgcatca gccgcggttc gtgctggcgg
      841 gtgcgccgcg gcagcagcag cagcgagagt gccggagtca ccgtcagcgc caccgccagc
      901 gaggccagca ccgcggcgat gtacccaatg cccagcggcg cgaacaggcg gcccgcgatg
      961 ccggacagcg tgacgatcgg caggaacacc aaaatcacgg caaaggttgc atagacaacg
     1021 gctgtgcgta cttcgaaggt cgcttccagc accacgagcg cggccggtcg cggcactgtg
     1081 gcgcgcgcgt tctcccgcaa ccgccgggtg atgttttcga taccgatgac ggcgtcatcg
     1141 acgacctcac ccagcgcgat cgccaggccg ctcagcgtca tcgtgttcag cgtgatgccg
     1201 aaataatcaa tcacgatcac ggcggcaagc aacgagatgg gtatggcgag gcagcagatg
     1261 gcagccgcgt agaggtccgc caggaacagg aacagcacgg caatcaccag cagaccgccg
     1321 atgaccagcg acgaacgcag gttgtccacc gcaacggtga tgaaattcgc cggccggaac
     1381 agccggccgt gcagcaccgc cccggcacgg tccaggctgg gccgcagatc gccgagcgca
     1441 tgttcgatgc cgcgggtcac ttccagcgtg ttggcgccgt attgctcgtc gatgttcatt
     1501 accacgccgg gctggcccat gatcgcggca ccgccgaccg ccggcatggg tgcctcgatc
     1561 acatcggcga cgtcgcccag cgtgatggcg ccgccgtcgc gccaagccac cacggcctgc
     1621 gcgatatcgg ccggctgcgt ggcggcttcg gtgcgcaagg tgatgtcctg gttcggcgtg
     1681 gagagaagtc cggcgccgcg cacgcccacc gcctggcgtg ccgcggccag caccgcgccc
     1741 atatccaggc ggtatttgat cagcttgtcc gggtggacgc ggatctgggt cgcgcgttgg
     1801 tcgccgccga agatctcgac cgcggcaacg ccgggtacgg ccagcaggcg tgggcgcacg
     1861 gtccaggtgg cgaggctgcg cagttgcatc agcgacaacg atggggaggt gaggccaatc
     1921 accaggaccg tcgccatcga cgaggtcagc ggcgtcatgc gtggcggtcc ggttccggtc
     1981 ggcagagcgt tcgccgcggc agccaaccgc tctgcgacca actggcgggc gcggtacggg
     2041 tcggtggaca gatcaaaata gaccttgatg tccgacagcc cggcaatcga attggacgtg
     2101 gtgcgctgca gaccggcaac gccgccgatc gccgtctcca cgggccgcgt caccagtgtc
     2161 tccacctgct cggcggaaaa ccccggcgct tccgtctgaa cgtccacctg aggcggcgcg
     2221 aattccggaa aggcgtcata acgg
//
LOCUS       DASSZH010000600         5613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1446347, whole genome shotgun sequence.
ACCESSION   DASSZH010000600 DASSZH010000000
VERSION     DASSZH010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446347"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VFN42_12995"
     CDS             <1..448
                     /locus_tag="VFN42_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET8997578.1"
                     /translation="WTIPIPVLLSVTIGVLGAFAGLWLFGLPLNLYAQIGMVVLISLA
                     AKNGILIVEFAKEQRERGMPIQEAAALGARMRFRAVMMTSIAFICGLVPLVWAEGAAM
                     LSRRAVGTAVFAGMIAASSIGVFLIPMLYTTFQSIRETVKRRFGSS"
     gene            complement(574..1494)
                     /locus_tag="VFN42_13000"
     CDS             complement(574..1494)
                     /locus_tag="VFN42_13000"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET8997579.1"
                     /translation="MMKDALFGGVNAAVLTPMNDDLSIDLDRMAAHCRWLLANGCNGL
                     AVLGTTGEANSLGLSERLALLEGLVERGIPASKMLPGTGTTAIPDTVLLTKKAQEMGC
                     RGALMLPPFYYKNPSDDGLLAYFDQVIQRVGGDIKVYLYNFPQQSAIPFSVDFLSRLL
                     KMYPGKVKGIKDSSGNYENSLSYVQNFARDGFEVYAGDDTLLKPLLEKGGAGCITAAS
                     NVNCSVAAAVYAGWNTPAGAAAHEVLSATRKAVISVPMIPALKALVARNTGDPRWQNI
                     RPPHLKLTAQQLAQAYAAFDACGMQMAKAA"
     gene            complement(1491..1979)
                     /locus_tag="VFN42_13005"
     CDS             complement(1491..1979)
                     /locus_tag="VFN42_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HET8997580.1"
                     /translation="MAKQPPFWKAKRLEEMTREEWESLCDGCGRCCLHKLRDDDSQAL
                     TFTNVACRLLDLHSCQCSDYARRQRRVPDCVQLTPATVREVDWLPPSCAYRRLAEGKD
                     LAWWHPLVSGDPATVHQAGISIRGRAVNERRAGPFEHHIVDWPGRMPRARRQARTKET
                     NE"
     gene            2173..2889
                     /locus_tag="VFN42_13010"
     CDS             2173..2889
                     /locus_tag="VFN42_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997581.1"
                     /translation="MTPRDYSQVPVEQLARLLLERARTDSGLPRRTPANTNEPPPGWS
                     VPPEAQPCRPWFPDRLAEQPRFAGQERIEDLVSQAMAAAQEAEDIARAASRRANRGAA
                     VAMALGVFGALAGVGAGVGSLLCDTGARPPVQAAQAADPVQPAAATAAAIAGQTELAQ
                     PAAMQAEPDDGSALSQQEPPALAPATFVRVLPAVPSLPSYGASDDSGWAPAAPPPRDR
                     VGPQSGFGRVLGTLRRLRTG"
     gene            2949..4196
                     /locus_tag="VFN42_13015"
     CDS             2949..4196
                     /locus_tag="VFN42_13015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HET8997582.1"
                     /translation="MAGKQHGFGATWDDAWLLDGVRTAFVDYTGAFAQVSPTDLGIKA
                     ARATLAKAAVPGEHVGAVLTGNMAQASFDAFMLPRHIGLYAGVPIAVPAHMVQRVCGT
                     GIELLMQAADAVTHRDTGVALCVGTESMSRNPVAAYTHRNGFRLGQIEFKDFLWEALL
                     DPAPGVTMGGTAENLARQYQITRQEVDAYAARSFDRALAAQRSGILAEEIVPVASERF
                     ELPGHAAREIRLPRSVPDVAQDTHVRPSPVDVLARLPPAFGGVQTGGNSSAIVDGAAA
                     AVVCSGDYARGQGRAPLARLVAGVAIGCAPEVMGIGPVPAIRALLDRSGLTLDAIDRI
                     EINEAFAAQVMACARALGLDEDKLNVNGGAIAIGHPLAATGVRLALTLARELRRSGLR
                     RGIASACIGGGQGIALLVENPAA"
     gene            complement(4213..5415)
                     /locus_tag="VFN42_13020"
     CDS             complement(4213..5415)
                     /locus_tag="VFN42_13020"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8997583.1"
                     /translation="MGQRDATTSRSGWLLLGLLWLLGADLRLTILAVPPVLPLIHHDL
                     HLDEKLVAALTGLPVLLFGVIAIPGSLLIARIGARRAAIAGVLIVSIASALRGVGPSV
                     PMLFGMTALMGAGVAIMQPALPTLVGRWFPQRISLATALYANGLLVGEMLPASLTIPL
                     VLPALGGSWEASFVFWAAPVLATAFLVLFLTPHTEPSRRSAPARWWPDWHDVRTWQLG
                     CMLGGGSALYFGCNAFLPDYLHAIGRADLLNAALTALNSGQIPASLLVMAVGKHLVGR
                     KEPFIVMSVIGLLCLAGLLVPAAPVIVLSAGLIGFAAAFTLILSLALPPLLAETHDVH
                     RLAAGMLALGYTITFIVPYIAGAVWDATHLTASALIPGVAGDLLVIGVAATFRRTPAT
                     HPLPEASR"
BASE COUNT          876 a         1965 c         1932 g          840 t
ORIGIN      
        1 ctggaccatc ccgatcccgg tgctgctgtc ggtgacgatc ggtgtgctgg gtgccttcgc
       61 cgggttgtgg ctgttcgggt tgccgctgaa cctgtacgcg cagatcggca tggtggtgct
      121 gatctcgctg gcggcaaaga acggcatcct gatcgtcgag ttcgccaagg agcaacgcga
      181 acgcggcatg ccgatccagg aggccgccgc gctcggcgcg cgcatgcggt tccgcgcggt
      241 gatgatgacc tcgatcgcct tcatctgcgg actggttccg ctggtatggg ccgagggtgc
      301 ggcgatgctg agccgccgag cagtggggac cgcggtgttc gccggcatga tcgcggcgag
      361 ctcgatcggc gtgttcctga tcccgatgct ctacaccacc ttccagtcga tccgggaaac
      421 cgtgaagcgg cggttcggat ctagctgaca cggcggtcgc gaagactcct ccccctcccc
      481 ttgcgggagg gggagggggg gaggggtcgt gcgggcagtc cccgcgagcg cgcatgccgc
      541 cccagagagg cgtgatgtct tccgccccct cggctacgcc gccttcgcca tctgcatgcc
      601 gcaggcatcg aacgcggcat aggcctgcgc gagctgctgc gccgtcagct tcaggtgcgg
      661 cggacggatg ttctgccaac gcggatcgcc ggtgttgcgc gccaccagcg ccttcagcgc
      721 cggaatcatc ggcacggaga tcactgcctt gcgggtcgcg ctgagcactt catgcgccgc
      781 cgcaccggcc ggcgtgttcc atcccgcgta caccgccgcc gccacgctgc agttcacgtt
      841 cgacgcggcg gtgatgcagc ccgcgccgcc tttctccagc agcggcttca gcagggtatc
      901 gtcgcccgca tacacctcga atccatcgcg cgcgaagttc tgcacgtagg acaggctgtt
      961 ctcataattg ccgctgctgt ccttgatgcc tttcaccttg ccaggataca tcttcagcaa
     1021 gcgcgacagg aaatccacgc tgaacggaat cgcggactgc tgcgggaaat tgtacagata
     1081 taccttgata tcgccgccca cgcgctggat cacctggtcg aaatacgcca gcaacccgtc
     1141 atccgagggg ttcttgtaat agaacggcgg cagcatcagc gcgccccggc agcccatctc
     1201 ctgcgccttc ttcgtcagca gcacggtgtc cgggatcgcc gtggtgccgg tgccgggcaa
     1261 catcttcgat gccgggatcc cgcgctccac caagccttcc agcagtgcca ggcgttcgct
     1321 gagccccagg ctgttcgcct cgccggtggt gcccagcacg gcgaggccgt tgcagccatt
     1381 ggccagcagc caccggcaat gcgcggccat gcgatccagg tcgatcgaca ggtcgtcatt
     1441 catcggcgtc agcacggcag cattcacgcc cccgaacagc gcgtccttca tcattcgttg
     1501 gtctccttcg tcctggcttg ccggcgggct cgcggcatgc ggcccggcca gtccacaatg
     1561 tgatgctcga aagggccggc ccggcgttca tttaccgccc ggccgcggat cgagataccc
     1621 gcctggtgga ccgtcgccgg atcgccggaa accagcggat gccaccaggc caggtccttg
     1681 ccttccgcca gccggcgata cgcacaggac ggcggcagcc agtccacttc ccgcaccgtg
     1741 gcgggcgtca gttgcacgca gtccggcacg cgccgctgcc ggcgggcgta atcgctgcac
     1801 tggcagctgt gcagatcaag cagccggcac gcaacgttgg tgaaagtcag cgcctggctg
     1861 tcatcgtcgc gcagcttgtg caggcagcag cggccgcagc cgtcgcacag cgactcccac
     1921 tcctcgcgcg tcatctcttc cagccgtttg gctttccaga agggaggctg tttggccatg
     1981 gcggcaaact acaggctggc ccggttgctg gccagcgcag gccgtcgccg cttcgccagt
     2041 cgcaggatgg tgactcgcgg gaattcccgt gcctgggcaa cctgataccg ctttggcagt
     2101 tccaaccatt gttcctccgt atggatggcg tggcccggcg cgtccggagg acagcgcagc
     2161 ggagcacgcc acgtgacacc tcgcgactat tcccaggtcc cggtcgagca gctcgcgcgt
     2221 cttctcctgg agcgcgcccg taccgactcc ggcctgcctc gccggacccc ggccaatacc
     2281 aatgagccgc cgccgggctg gagcgtgcca cccgaggcac agccctgccg gccctggttc
     2341 ccggaccgcc tggccgaaca gccgcgattt gccgggcagg aacgcatcga ggatctcgtc
     2401 tcgcaggcaa tggcagcggc gcaggaagcg gaagacatcg cccgcgccgc cagccgccgc
     2461 gccaaccgtg gcgcggcggt ggccatggcc ttgggcgtgt tcggggcact ggccggagtg
     2521 ggcgcgggag tcggcagcct gctctgcgac accggcgcca ggccgccggt ccaggcggca
     2581 caggccgccg acccggtgca gcccgcggcc gcgacggcgg cagcgatcgc cggtcagacg
     2641 gagctggcgc agcccgccgc aatgcaggcg gagcccgatg acggcagcgc gctgtcgcag
     2701 caggagcccc cagcgctggc gccggccacg ttcgtgcgcg tgttgccggc cgttcccagc
     2761 ctgccatcct acggcgcttc ggacgattcc ggctgggctc cagccgcgcc accgccgcgg
     2821 gatcgcgttg gtccgcaatc cggctttggc cgcgtgctcg gcacgctgcg gcgcctgcgc
     2881 accggctgag gctggacagc caccttgccg cctgggttca atcccgccag gcaagggcgg
     2941 ggttggacat ggccggcaag cagcacggat ttggcgcgac ctgggacgac gcatggctgc
     3001 tcgacggcgt gcgcaccgcc ttcgtcgatt acaccggcgc cttcgcgcag gtttccccca
     3061 ccgatctcgg tatcaaggca gcccgcgcga ctctggcgaa ggccgcggtc cccggcgagc
     3121 atgtcggcgc ggtgctcacc gggaacatgg cgcaggccag cttcgacgcc ttcatgctgc
     3181 cgcgccacat cgggctctat gccggggtgc ccatcgcggt gccggcgcat atggtgcagc
     3241 gtgtctgcgg caccgggatc gagttgctga tgcaggcggc cgacgcggtc acccaccgcg
     3301 acaccggcgt ggcactgtgc gtcggcaccg aatccatgag ccgcaatccg gtggccgcct
     3361 acacccatcg caacggcttc cggctgggcc agatcgagtt caaggacttc ctgtgggagg
     3421 cactgctcga tccggcaccc ggcgtgacca tgggtggaac cgcggagaac ctggcgcgcc
     3481 agtaccagat cacccgccag gaggtggatg cctacgcggc ccgcagcttc gaccgcgccc
     3541 tggccgcgca gcgcagcggc attctggcgg aggagatcgt gccggtcgcc agcgaacgct
     3601 tcgaattgcc cggtcatgcc gcacgggaaa ttcgcctgcc gcgcagcgtg ccggacgtcg
     3661 cgcaggatac gcatgtgcgg ccgtcgccgg tggatgtgct ggccagactg ccgccggctt
     3721 tcggcggcgt gcagaccggc ggcaacagct ccgccatcgt ggatggcgcc gctgcggcgg
     3781 tggtgtgctc cggcgactat gcgcgcgggc agggcagggc gccgctggcc cggctcgtgg
     3841 ccggcgtcgc gatcggctgc gcgccggagg tcatgggcat cggtccggtc ccggcgatcc
     3901 gggctctgct ggaccgcagc ggactgacgc tcgacgcgat cgaccgcatc gagatcaacg
     3961 aggccttcgc cgcccaggtc atggcctgcg cccgcgcgct ggggctggat gaggacaagc
     4021 tgaacgtgaa cggcggcgcc atcgcgattg gccatccgct ggcggcgacc ggcgtacggc
     4081 tcgcactgac gctggcgcgc gaattgcggc gcagcggact gcgccgcggc atcgcctcgg
     4141 cctgcatcgg cggcgggcag ggcatcgcgc tgctggtgga gaacccggcg gcgtagcggc
     4201 gccgcctggc gttcagcggg acgcctccgg cagcgggtgc gtggcgggcg tgcgccggaa
     4261 ggtggccgcc acgccgatca ccagaaggtc gccggccact cctgggatca gggccgacgc
     4321 ggtcaaatgc gtcgcatccc acacggcgcc ggcgatgtag ggcacgataa aggtgatggt
     4381 gtagcccagc gccagcatgc cggccgccag ccggtgcacg tcgtgcgtct ccgccagcag
     4441 cggcggcagg gccagtgaca ggatcagcgt gaacgcggcg gcaaagccaa tcaacccggc
     4501 ggacagcacg atcaccggcg ccgccggcac cagcagcccc gccaggcaca gcaggccgat
     4561 caccgacatg acgatgaatg gctccttgcg tccgaccagg tgcttgccca ccgccatgac
     4621 cagcagcgag gccgggatct gtccgctgtt cagcgcagtc agcgcggcat tcagcagatc
     4681 ggcccggccg atcgcgtgca ggtagtccgg caggaatgcg ttgcagccga aatacagggc
     4741 ggatccgccg cccagcatgc agccgagctg ccaggtacgc acgtcgtgcc agtccggcca
     4801 ccagcgtgcc ggggcggaac ggcggctggg ttccgtatgc ggcgtcagga acagcaccag
     4861 gaacgcggtg gccagcaccg gcgcggccca gaacacaaag cttgcttccc aactgccacc
     4921 cagcgccggc agcaccagcg ggatggtcag gctggccggc agcatctcgc cgaccagcag
     4981 gccgttggca tacagggccg tggccagcga aatgcgctgc gggaaccagc ggccgaccag
     5041 ggtgggcagc gcgggctgca tgatggccac gccggcgccc atcagcgccg tcatgccgaa
     5101 cagcatcggc acggacgggc ccactccgcg cagcgccgag gcgatggaca caatcagcac
     5161 gccggcaatg gctgcccggc gcgcaccgat ccgggcgatc agcagcgagc cgggaatggc
     5221 gatcacgccg aacagcagca caggcagccc ggtcagcgcc gccaccagct tctcgtccaa
     5281 atggagatcg tgatggatca gcggcagcac cggtggcacg gccaggatcg tcaggcgaag
     5341 atcggccccg agcagccaca gcagaccgag cagcagccag ccggaccggc tcgtggttgc
     5401 gtcgcgctgg cccatgcgga gtcgttccct tcccgtcggt cggccattct cgccgccgca
     5461 tcccgggctg caatgggtcc cgggacagca agggcaggtc aatcatggtc cgtccggaag
     5521 ctcgccagca acggtgtggg atcgatggcg ggaccttggt tcgcgagcca gcggcgcaaa
     5581 tccggcctcg gcagcccggc ctcggccaag tac
//
LOCUS       DASSZH010000601         2501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1565849, whole genome shotgun sequence.
ACCESSION   DASSZH010000601 DASSZH010000000
VERSION     DASSZH010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565849"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..936
                     /locus_tag="VFN42_13025"
     CDS             133..936
                     /locus_tag="VFN42_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997584.1"
                     /translation="MTQANRNPGLPPASQPSNTSSTETLPEGIAAVLAVLQILLTYGN
                     HLLATLEHRAVQRSFATIAQCFGTARLPVILARLTRGIRRALALQRVLLARAATGNDL
                     VPEQPAPYRPSRERSSAAAGGRQRQTSDPADRPDPEEQTDLSTIPSIAQLEAEIRDQS
                     VGRVLADICRDLGVSPFRCEQRFAASLHQAITTYGGDHLDLCGEMLNRSLELLKELDR
                     TRDPALDWPKGGAETVRRIMGFSVGEPPVCPFPPANSWGIPEAAAAAPS"
     gene            complement(1160..1681)
                     /gene="pcaG"
                     /locus_tag="VFN42_13030"
     CDS             complement(1160..1681)
                     /gene="pcaG"
                     /locus_tag="VFN42_13030"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HET8997585.1"
                     /translation="MPFATASQTIGPYWHLIEDKSWSDLTRFGAEGERITLTGRVLDG
                     DGAPCADACVEIWQSDPPASDVFPGFGRSVADASGAFRFVTLKPGPVPGRGNAQQAPH
                     IAICILSRGLMKGLVTRAYFQGEALNETDPLLMSLEDPARRATLIARPDGPGIWRLDI
                     RLQGDRETVFLEV"
     gene            complement(1684..2388)
                     /gene="pcaH"
                     /locus_tag="VFN42_13035"
     CDS             complement(1684..2388)
                     /gene="pcaH"
                     /locus_tag="VFN42_13035"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HET8997586.1"
                     /translation="MSDPAGFRPYAPGTQPPYDVPAYRGTTKRHPQQALHPLPPSITE
                     TSGPRFSPARFPPSADLTSNTTGKPALGERIIVAGRVLDEDSRPVRNTMVEIWQCNAT
                     GRYDHPADQHDAPLDPNFHGAGRVFTDEDGNYRFLTIRPGAYPWPNHHNAWRPNHIHY
                     SLFGPAFATRLVTQMFFPGDPLLAFDPIFNGVPDAHARNRLIAQFDLELTQPDYALGY
                     RWDIVLRGSAATPMEE"
BASE COUNT          399 a          931 c          778 g          393 t
ORIGIN      
        1 gcatctgcgg caaaaaatct tgcttgctgc tgccgctctc cgcgcttcgc gctgcccggt
       61 tcacccgatt gccctgcatc gctgcgaaat cgtgcttgac ttgtctaact ggattggtta
      121 tgatgtctaa tcatgaccca agcgaaccgc aaccccggcc tgcctcccgc atcccaaccc
      181 tccaacacaa gctccaccga aaccctgccg gagggaatcg ccgcggtcct cgctgtcctg
      241 caaatcctgc tcacctacgg caaccacctg ctggccacgc tggaacaccg tgcggtccag
      301 cgcagcttcg ccaccatcgc gcagtgcttc ggcaccgcgc ggcttcccgt catcctcgct
      361 cgtctgaccc gcggcatccg ccgcgccctg gcgttgcaac gcgtgctgct ggcgcgggcc
      421 gcgaccggca acgacctggt ccccgagcaa cctgcccctt accggccgtc gcgggagcgg
      481 tcatccgcgg cagcgggcgg acgtcaacgt cagacgtccg atcccgctga ccgccccgat
      541 ccggaggaac aaaccgacct ttccaccatc cccagcatcg cgcagctcga agcggaaatc
      601 cgcgaccaat cggtcggccg ggtgctcgcc gatatctgcc gcgacctcgg cgtctcgccg
      661 ttccgctgcg aacaacgctt cgcggcatcg ctgcatcagg cgatcaccac ctacggcggc
      721 gaccatctcg acctgtgcgg cgaaatgctc aaccgcagcc tcgagctcct caaggaactc
      781 gaccggaccc gcgatccggc gctcgactgg ccgaagggtg gtgccgagac ggttcgccgc
      841 atcatgggat tcagcgtcgg cgaaccgccg gtctgtccgt ttccgccggc aaattcatgg
      901 ggaatcccgg aggccgcggc agccgcacca tcctgaccgc gtagcacact gacagctcgc
      961 accacgcacg ggaccggaca ccggacggcc ccgccgtccg gattcgcgcg gattgcatgc
     1021 cgcgccgcgg atgcggaccg cagctccctc ccccgccccc tcgcgggcgc ggtcggtccc
     1081 cgcaaattca cgcacgcccc ctccccccaa ccccctctcg caagcggcga cggagatcct
     1141 ttggcctgct ctccggaggc tacacctcca ggaacaccgt ctccctatcg ccctgcagcc
     1201 tgatgtccag ccgccagatc cccggcccgt ccggccgcgc gatcagcgtg gcccgacgcg
     1261 cgggatcctc cagcgacatc agcagcggat cagtctcgtt gagcgcctcg ccctgaaagt
     1321 aggcccgcgt caccagcccc ttcatcaacc cgcgtgacag gatgcaaatg gcgatatgcg
     1381 gcgcctgctg cgcgttgccg cggcccggca ccggccccgg cttcaacgtc acgaagcgga
     1441 acgcgccgct cgcgtccgcc acgctgcggc cgaaccctgg gaacacatcc gacgcaggcg
     1501 gatcggactg ccagatctcc acgcaggcat ccgcgcaggg cgcgccgtcc ccgtccagca
     1561 cgcggccggt cagcgtgatc cgctcccctt cggcgccgaa ccgcgtcagg tcggaccaac
     1621 tcttgtcctc gatcaggtgc cagtacgggc caatcgtctg gctggcggtg gcgaacggca
     1681 tccttattcc tccatcggcg tggcggcgct gccgcgcagc acgatgtccc agcggtagcc
     1741 cagcgcgtag tccggctggg tcagctccag gtcgaactgc gcgatcagcc ggttgcgcgc
     1801 atgcgcatcg ggtacgccgt tgaagatcgg gtcgaacgcc agcagcggat cgcctgggaa
     1861 gaacatctgc gtcaccagcc gcgtggcgaa ggccgggccg aacagcgagt aatggatatg
     1921 gttcggccgc caggcgttgt ggtggttcgg ccacggatag gcaccaggcc ggatggtcag
     1981 gaaccggtag ttgccgtcct catcggtgaa cacccgcccc gcgccatgga aattcggatc
     2041 cagcggtgcg tcgtgctggt cggccgggtg gtcgtagcgg ccggtggcat tgcactgcca
     2101 gatctccacc atggtgttgc gcaccggccg gctatcctca tccagcacgc ggcccgcgac
     2161 gatgatacgc tcgcccagcg ccggcttgcc ggtggtgttg ctggtgaggt cggcggaggg
     2221 gggaaaccgc gccggcgaaa accgtggacc gctggtctcc gtgatgctcg gcggcagcgg
     2281 atgcagcgcc tgctgcggat gccgcttggt ggtgccgcga tacgccggca cgtcataggg
     2341 cggctgcgtg ccgggcgcgt aggggcggaa gcctgccgga tcgctcatgc tctgcgctcg
     2401 ctgactgggt gccataccgt ctcacgccgc ccgcatcggg gcaaccggac gcggcgctgg
     2461 catgcacgcg caaacacgct atcgatgcac cggcgcaccc g
//
LOCUS       DASSZH010000602         5311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_747408, whole genome shotgun sequence.
ACCESSION   DASSZH010000602 DASSZH010000000
VERSION     DASSZH010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747408"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..295
                     /locus_tag="VFN42_13040"
     CDS             146..295
                     /locus_tag="VFN42_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997587.1"
                     /translation="MDDIRTEHDSSLTERKPEPAADNTVPPERHKVGPESEVRGGPEA
                     PEERE"
     gene            complement(318..1184)
                     /gene="motA"
                     /locus_tag="VFN42_13045"
     CDS             complement(318..1184)
                     /gene="motA"
                     /locus_tag="VFN42_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010666247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HET8997588.1"
                     /translation="MIPLGALVFLLLCVFGSYVVSGGAIAPLAEALPFELWTIGGAAI
                     GIFVMSNSMHDLKHALGNFRKILKGAAFHKTDYVDLLSLLYFLMRLASTKGNMALEPH
                     IEKPDESAAFQQFPRILGNRFATATICDYLRMVGMNADDPNQIEDVMGRELKKTLNEE
                     LHGAHALQSLADGLPALGIVAAVLGVIKTMSHIDSPPAVLGGMIGSALTGTFLGVLLA
                     YGFAGPFAARLTSVVEEEAKYYEVIRAVLVTHLHGNAPQVSVESGRKMVPNQHMPSFT
                     ELEEAVQSVHVG"
     gene            complement(1194..2294)
                     /locus_tag="VFN42_13050"
     CDS             complement(1194..2294)
                     /locus_tag="VFN42_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HET8997589.1"
                     /translation="MRAAMAERMGGKRDKAKGKATVVIRKEEAAPPAAHGGAWKVAYA
                     DFVTAMMAFFLLMWLLNATTEEQRRGLADYFAPSNLFSHGSSGTGDPFGGHTAFDQGA
                     MVSDRGAVQVVPGHRPAATDPGDSAAQQASVAGNADREAGSATTVKPDLPAARPVAQP
                     DRPAGPNAPAGVVPDGPPDGPAAGQALQPQSANPAAQEKAAFEAAAREISAAVQGDPD
                     LAELAKQLAIDITPEGLRIQIMDEAQRPMFALGSADPNQHTRQLLAKVAPVLLRLRHA
                     ISIAGYTDAARFPGPERTNWELSTERANTTRHVLVEDGFPEALVTSVTGHADHDLLLP
                     ADPLAAANRRVAIVVLRTASPHAAAKPAAPAQ"
     gene            complement(2365..4887)
                     /locus_tag="VFN42_13055"
     CDS             complement(2365..4887)
                     /locus_tag="VFN42_13055"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET8997590.1"
                     /translation="MPCRFDSDADAARSGPAETARRGASPPVAAFQAICNAIPVGLCL
                     IDRSLRFVAMNRLLAQMTGRSIDESIGRGLAEVTPGVAAQLDPALRRVLEGERLVDLE
                     LRGTEVGTVCEGRVFLASLEPMPGPDGGVAAVLCTVLDITRRKRAELALRDSEIRFRG
                     SFDQAAVGIAHIEFNAQWKWINRKFCEMLGYTQAELTAMTFVDLSHPEDLQDDLRQAA
                     RLRAGEISTYSMEKRYFRKDGSVLWGQLTVSLLRDEAGVPQNYVVFVEDISARKAVES
                     ALADSESRLKRAIAIGGMAAWEWDVASDDLYASASLDELLGQPAGSLRTMQAAMQVVH
                     PDDRPAVAAAIALALQGGAEDDEVEFRVVWPDGTLRWLHARGRVEERNAGAPRVVGLT
                     YDITGRKESEARITWLAHYDPLTRLANRTLFRQRLEQTLAGLDERRGMVALHWMDFDA
                     FKCINDTLGHPAGDALLREAAARLQLCLSSSDLAARVGGDEFAVIQTGIHGPADAEAL
                     GASMLEAMAEPYEIEGRQLSVGASLGIALVPRDSRDPDEIVKKADIALYRAKADGGGA
                     IRLFSPEMDAAVRRRQEVIARVRRGLAEDRLALHFQPLVSLHSGMVTCAEALLRGTDG
                     IPYIGSVPEFISCAEEAGLIVPLGRWVLRSACQEAARWDDKLRVAVNLSPVQFSSPGL
                     LAAVTSALDESGLPPERLELEITESVLLHHNQANVAILHQLRALGVRIALDDFGTGYA
                     SLSYLHRFPFDKIKIDQSFVCGLPGEAKASAIVRAVIGLGRSLGIAIAAEGVETTSQL
                     ESLRNEGCDEVQGFLLSRPVPADQIAVVADSLERDAAAAVLF"
BASE COUNT          873 a         1851 c         1745 g          842 t
ORIGIN      
        1 gtggtcttcc ccgccccctc gccgccttcc agggtgatga aacagccgcg cattccgtcc
       61 ctcgccgcat cgtgacgcag cggtagcaca ggattcggcc ggcaggcatg cgcaacccgg
      121 cacgattccg cgggttactc cggccatgga cgatatccgc accgagcacg acagcagcct
      181 gacggaacgc aagccggaac cagcggcaga caacaccgtt ccgccggagc ggcacaaggt
      241 cggtccggaa tccgaggtgc gcggcgggcc ggaggcaccg gaggagcggg agtaacgggt
      301 cccaggccct cgcgggccta tcccacatgg accgactgca cggcctcctc cagctcggtg
      361 aagctaggca tgtgctggtt gggcaccatc ttgcggcccg attccacact gacctgaggc
      421 gcgttgccgt gcagatgggt gaccagcacg gcgcggatca cctcgtagta cttcgcctcc
      481 tcctccacca cgctggtcag ccgggcggcg aaggggccgg cgaagccata ggccagcagc
      541 acccccagga aggtgccggt caaggcgctg ccgatcatgc cgcccagcac cgcgggtggg
      601 ctgtcgatgt gcgacatggt cttgatcacg cccagcacgg cggcgacgat gcccagcgcg
      661 ggcaacccat ccgccaggct ctgcagggca tgcgccccgt gcagctcctc gttcagcgtc
      721 tttttcagct cgcgccccat cacgtcctcg atctggttcg gatcgtcggc gttcatcccc
      781 accatacgca ggtagtcgca gatcgtcgcg gtcgcgaacc ggttgcccag gatgcgggga
      841 aactgctgga acgcggcgct ctcgtccggc ttctcgatat gcggttccag cgccatgttc
      901 cccttggtgc tggccagccg catcaggaaa tacagcaggc tcagcaggtc gacgtaatcg
      961 gtcttgtgga acgccgcacc tttcaggatc ttgcggaaat tgcccagcgc atgcttcagg
     1021 tcatgcatgc tgttcgacat gacgaaaatg ccgatggcgg cgccgccgat ggtccacagc
     1081 tcaaacggca gcgcttcggc gagcggcgcg atcgcgccac cggaaaccac atagctgccg
     1141 aacacgcaca gcagcaggaa caccagggcg ccgaggggga tcatcgtgtg cgctcactgc
     1201 gccggtgccg ccggtttcgc ggcggcgtgc ggactcgccg tgcgcaggac cacgatggcg
     1261 acccgccggt tcgccgcggc cagcggatcg gcaggcagca gcaggtcatg gtcggcatgg
     1321 ccggtgacgc tggtgaccag tgcctccgga aagccgtcct ccaccagcac atgccgcgtg
     1381 gtgttggcgc gttcggtgga aagctcccag ttggtgcgct caggaccggg gaaccgcgcg
     1441 gcatcggtgt agccggcgat ggagatggcg tgccgcagcc gaagcagtac cggcgccacc
     1501 ttcgccagca attgcctggt gtgctggttc ggatcggcgg agccgagtgc gaacatcggc
     1561 cgctgcgcct cgtccatgat ctggatacgc aggccttccg gcgtgatgtc gatggcgagc
     1621 tgcttggcca actccgccag atcgggatcg ccctgcaccg ccgcgctgat ctcgcgggcg
     1681 gccgcttcga aagcggcctt ctcctgcgcg gccggattgg cggactgggg ctggagggcc
     1741 tgcccggcgg ccggtccatc cggcgggcca tccggcacca cgccggcggg cgcgttcggt
     1801 ccggcgggtc ggtcaggctg agccaccgga cgggcggccg gcagatcggg tttgacggtg
     1861 gtggcactgc ccgcctcccg atcggcgttg ccggcgacgg acgcctgctg ggcggccgaa
     1921 tcacctggat cggttgccgc cggtcggtgc ccgggaacca cctgcaccgc gccgcggtcc
     1981 gaaaccatcg cgccctggtc gaacgcggtg tgaccgccaa acggatcgcc ggtaccggag
     2041 gagccatggc tgaacaggtt gctgggggcg aagtagtcgg ccagtccccg gcgctgctcc
     2101 tcggtggtgg cgttcagcag ccacatcagc aggaagaagg ccatcatggc agtgacgaaa
     2161 tcggcgtagg ccaccttcca ggcgccgcca tgtgccgctg gtggcgcggc ctcctccttg
     2221 cggatgacga ccgttgcctt gcccttggcc ttgtcccgct tgccgcccat gcgttccgcc
     2281 attgccgctc gcatcaggca gcatgcgccg gttcggttaa cgttcggtga tcatcgggca
     2341 gcaagcggcc gagcggcccg ggcgctagaa cagcacggcc gccgctgcat cgcgttccag
     2401 gctatcggcc accaccgcga tctgatcggc cggaaccggc cggctgagca ggaagccctg
     2461 cacctcgtcg cagccctcat tgcgcaggct ttcgagctgc gaggtggtct ccaccccctc
     2521 ggccgcgatc gcaatgccca ggctgcgccc cagaccgatc accgcgcgca caatggcgct
     2581 cgccttggcc tccccgggca ggccgcacac aaaggactgg tcgatcttga tcttgtcgaa
     2641 cggaaaccgg tgcaggtagc tcaacgaggc gtaccccgtg ccgaaatcgt ccagcgcaat
     2701 ccggacgccc agtgcgcgga gctggtgcag gatggccacg ttcgcctggt tgtggtgcag
     2761 cagcacggat tcggtgatct ccagctccag ccgttccggc ggcaggccgg attcgtccag
     2821 tgcgctggtc accgcggcga gcaagcccgg gctgctgaac tgcaccgggg aaaggttgac
     2881 ggcaacgcgc agcttgtcgt cccagcgtgc cgcctcctgg cacgcgctgc gcagcaccca
     2941 gcggccgagc ggcacgatca ggccggcctc ctcggcgcag gagatgaact cggggaccga
     3001 accgatgtac ggaattccat cggtcccgcg cagcagcgct tccgcgcagg tcaccatgcc
     3061 gctgtgcagc gaaaccagcg gctggaaatg cagtgccagc ctgtcctcgg ccagaccgcg
     3121 gcgaacccgg gcgatcacct cctgccggcg gcgcacggcg gcgtccatct ctggcgagaa
     3181 caggcgaatg gcgccgccgc catcggcctt ggcacggtac agcgcgatat ccgccttctt
     3241 gacgatctcg tccggatcgc ggctgtcgcg cggaaccagg gcgatgccga ggctggcgcc
     3301 gacgctgagc tgccgccctt cgatttcata gggttcggcc atcgcttcca gcatgctggc
     3361 gcccagcgct tccgcatccg ccgggccatg aatgccggtc tggatgacgg cgaattcgtc
     3421 gccgccgacc cgggccgcga gatccgagct ggacaggcac agttgcagcc gcgcggcggc
     3481 ctcacgcaac aaagcgtcgc cggccggatg gccaagcgtg tcgttgatgc acttgaacgc
     3541 gtcgaaatcc atccagtgca gcgccaccat gccgcggcgt tcatccagcc cggcgagcgt
     3601 ctgctccagg cgctggcgga acaacgtacg gttggcgagc cgggtgagcg gatcgtagtg
     3661 cgccaaccag gtgatgcggg cctcgctctc cttgcggccg gtgatgtcat aggtgaggcc
     3721 aaccacacgc ggtgcaccgg cattcctctc ctcaacccgg ccccttgcat gcagccagcg
     3781 gagcgtgccg tcgggccaca cgacgcggaa ttcgacctcg tcatcctcgg ccccgccttg
     3841 cagcgccagc gcgatcgccg cggcgaccgc cggccggtcg tcgggatgca cgacctgcat
     3901 cgccgcctgc atcgtccgca acgacccggc gggctgtccg agcaattcgt ccaggctggc
     3961 ggacgcgtag agatcgtcgc tcgccacatc ccattcccag gcagccatcc cgccgatcgc
     4021 aatggcccgc ttcaggcgcg attcgctgtc cgccagggcc gattcgaccg ccttgcgcgc
     4081 ggagatgtcc tcgacaaaga ccacgtagtt ctgcggcacc ccggcttcgt cgcgcagcag
     4141 cgagacggtc aactgccccc acagcacgct gccgtccttg cggaaatacc gcttctccat
     4201 gctgtaggtg gaaatctcac ccgcgcgcag gcgcgctgcc tgccggagat cgtcctgcag
     4261 gtcctcggga tgcgacaggt ccacaaacgt catcgccgtc agctcggcct gcgtatagcc
     4321 aagcatctcg cagaatttcc ggttgatcca tttccattgc gcgttgaatt cgatatgcgc
     4381 gatgccgacc gccgcctggt cgaagctgcc gcgaaagcgt atttcgctgt cccgcagcgc
     4441 cagttccgcc cgcttgcggc gggtaatgtc cagcaccgta cacagcaccg ctgcaacgcc
     4501 cccatccggg ccgggcatcg gctccagcga ggccaggaac acccggccct cgcagaccgt
     4561 gccgacctcg gtgccgcgca gctccaggtc gaccaggcgc tccccctcca ggacccgccg
     4621 caacgcggga tcgagctggg cggccacgcc cggcgtcacc tccgccaggc cgcggccaat
     4681 gctttcgtcg atcgatcgcc cggtcatctg cgccagcagc cggttcatgg cgacgaagcg
     4741 cagcgaacga tcgatcaggc agagtccgac cggaatcgca ttacagatcg cctggaatgc
     4801 cgcaacgggc gggctggcgc cgcgccgggc ggtctcagcg ggcccagacc gagcggcgtc
     4861 tgcgtcgctg tcgaatcggc acggcatcca tggtctcctt ggaccgcaac ttcacaggga
     4921 aaagttacag gtaagttcca acgcgatcga agcgggggcc acgtacatgc ggggcgcggg
     4981 agatttcgcc ttggcgctgc cagcgacgga tcgctttgtg gctccggtgg ggcgcggcgt
     5041 tcccggaaga ggcgtgggag gtactcacgg cgccccatct aacaccgggt agatcgcgca
     5101 tccagctata attgccggaa aatccgagcc gaatgagact tcgcccggct aggtcgctcc
     5161 cgccgctccg gagtgcttca ggtcaaaccg agggaatgaa gccatgtatt cacatttttg
     5221 cgcgccggcc ggaaacaggg cggcataatc tcgcattcgg aaatgccggc actctgccgc
     5281 cgatcgcggc ggcggccagc cgcagcgctg c
//
LOCUS       DASSZH010000603         3346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_372542, whole genome shotgun sequence.
ACCESSION   DASSZH010000603 DASSZH010000000
VERSION     DASSZH010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372542"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VFN42_13060"
     CDS             complement(<1..516)
                     /locus_tag="VFN42_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HET8997591.1"
                     /translation="MAVEPDWLVWAREMQALAQTGLTFTKDPYDAERYGKLRGLALRV
                     LAAHTGAASTRLEGLFAGEAGYATPKLGVRGAVFDHAGRILLVREAADSGRWSLPGGW
                     VDVNQTPAESVVREVAEESGYRVRVEKLAAAWDKARQGHPPGPFSVLKLYFICALEGG
                     EARTSLETTEVA"
     gene            complement(519..1301)
                     /locus_tag="VFN42_13065"
     CDS             complement(519..1301)
                     /locus_tag="VFN42_13065"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HET8997592.1"
                     /translation="MTYKPPRRPQFFYTTAPLPCPYVPGRTERKVVTEITGPDADALH
                     DRLSRAGFRRSHNIAYAPVCPSCQACVPIRIPVADFQPDRTLRRLARANTMVEGFEVP
                     ARATAEQFHLFQRYQAVRHGEGDMATMSFYDYRAMVEDTPIETFMVEFRDPDDVMVGT
                     CLTDRLGDGLSAVYSFFAPGLEKRSLGTYTILWLIERAKALGLPYVYLGYWVPESRKM
                     AYKARFRPTEILSGGVWRVLTEADLEGAEPLENGVLVPASAT"
     gene            complement(1298..1807)
                     /locus_tag="VFN42_13070"
     CDS             complement(1298..1807)
                     /locus_tag="VFN42_13070"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HET8997593.1"
                     /translation="MSDSTLPWFADDMLTHGVLWRRVGGWFVDAVLIGLLVFVLWWLL
                     VLFGFLTLGLGFPLLGVLPLVPFLYQLLSMLGPGSATPGQQVFGLVVRRNDDLGPPMP
                     LQVVLFILGFAVTMATCGLLLAVALVTVRHRTLHDLVSGLVVVRVRGMYALTAPPGSW
                     NMPGGQSYS"
     gene            complement(1804..>3346)
                     /locus_tag="VFN42_13075"
     CDS             complement(1804..>3346)
                     /locus_tag="VFN42_13075"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HET8997594.1"
                     /translation="GEHSKTGAPLLAGDPHLQFGFPGIWYLARIDTPDHTLVGATGPG
                     VPGIILGRNRHIAWTFTTTGADVQDIFIEQPAGPGEYQTPDGPRPFHIREERIKVRGQ
                     PDVMLKVRSTRHGPVISDLLPGKQPILAVAMANLAPGDTAAEGLFALDSAGSVAEAGK
                     AAALITSPVQNLLVADRDHIGLFVTGRVPIRKAGDGAAPVSGDGTYDWTGWASGDQLP
                     QYVDPASGVLVNANERIAPPDFPVFLGRDWFGDWRAKRIKQMLASGGPFTPEDFARMQ
                     VDVRSTFAQQVLPALLKVKPSQPGGASGKALALLRDWDGSMAMDLPQPLIFNAWMDRF
                     NEAVLKQHNIPLRDSGPQPDFVAYVLSPSGAHWCNGDCSALLQSSLDSAVNALSARFG
                     ADPDAWRWGIAHPAVFAHPILQHVPLLGPWSTISIPSPGDDTTVDAGSPVPDQFQSVH
                     GPSYRGVYDLADLDKSLFMMAPGQSGNLLSGYSRNFVKRWRDGATITIGPKPDRVTAT
                     IDLLP"
BASE COUNT          554 a         1183 c         1128 g          481 t
ORIGIN      
        1 ggccacttcc gtggtttcca ggctggtgcg cgcctcgccg ccttccagag cgcagatgaa
       61 atacagcttc agtaccgaga agggcccggg cggatggcct tgccgtgcct tgtcccaggc
      121 cgcggcgagt ttctcgaccc gcacgcgata gccggactct tccgccactt cgcgtaccac
      181 actctccgcc ggggtctggt tcacatccac ccagccgccg ggcagcgacc agcggccgga
      241 atccgccgcc tcgcgcacca gcaggatacg gccggcatgg tcgaacaccg cgccgcgcac
      301 gcccagcttc ggcgtggcat aacccgcctc cccggcgaac aggccttcca gacgggtgct
      361 tgcagccccg gtatgcgcag ccaggacccg cagcgccagg ccgcgcagct tgccgtagcg
      421 ctccgcgtcg tacggatcct tggtaaaggt cagcccggtc tgcgccagtg cctgcatctc
      481 gcgcgcccag accagccagt ccggctcgac ggccacgctc aggttgccga agccggcacc
      541 agcacgccgt tctccagcgg ttccgcgcct tccagatccg cctcggtcag cacccgccac
      601 accccgccgg agaggatctc ggtcgggcgg aagcgcgcct tgtaggccat cttgcggctc
      661 tccggcaccc agtagcccag gtacacgtag ggcaggccca gcgccttcgc ccgctcgatc
      721 agccacagga tggtgtaggt gcccagcgac cgcttctcca gccccggcgc gaagaagctg
      781 tagaccgcgg acagcccgtc ccccagccgg tcggtcaggc aggtgccgac catcacgtcg
      841 tccgggtcgc gaaactccac catgaaggtc tcgatcggcg tgtcctccac catggcgcgg
      901 taatcgtaga aactcatggt ggccatatcg ccttcgccgt gccgcaccgc ctggtagcgc
      961 tggaacaggt ggaactgctc cgccgtggcg cgggccggca cctcgaaccc ctccaccatc
     1021 gtgttggcgc gggcgagccg ccgcagcgtg cggtccggct ggaaatccgc caccgggatg
     1081 cggatcggca cgcaggcctg gcaggacggg cagaccgggg cataggcgat gttgtggctg
     1141 cgccggaagc cggcgcgcga cagccggtcg tgcagcgcgt cggcgtccgg cccggtgatc
     1201 tcggtcacca ccttccgctc ggtgcggccg ggcacgtacg ggcacggcag cggtgctgtc
     1261 gtgtagaaga actgcgggcg gcgcggaggc ttgtaggtca tgaatacgat tgtccgcccg
     1321 gcatgttcca ggagccgggc ggcgcggtca atgcgtacat gcccctaaca cgcaccacaa
     1381 cgagtccgct caccaggtcg tgcagcgtgc ggtggcgcac agtcaccagc gccacggcca
     1441 gcagcaggcc gcaggtcgcc atggtgacgg cgaatcccag gatgaacagc accacctgca
     1501 gcggcatcgg cgggccgaga tcgtcattgc ggcggaccac caggccgaac acctgctggc
     1561 ccggcgtggc gctgccgggg cccagcatgg acagcaactg gtagaggaac ggcaccaggg
     1621 gcagcacgcc gagcagcgga aaacccaggc cgagggtcag gaagccgaac agcaccagca
     1681 gccaccacag cacgaagacc agcagcccga tcagcacggc atcgacgaac cagccgccga
     1741 cgcggcgcca cagcacgcca tgggtcagca tgtcgtcggc gaaccaaggc agggtagaat
     1801 cgctcatggc agcaggtcga tcgttgcggt gacccggtcc ggcttgggtc cgatggtgat
     1861 tgtagcgcca tcgcgccacc ttttgacaaa attacggctg tacccgctga gcaaattgcc
     1921 cgattggccg ggcgccatca tgaacagcga cttgtcgaga tccgccaggt cgtacacgcc
     1981 gcgataggat gggccgtgca ccgactggaa ctggtcgggc accgggctgc cggcgtccac
     2041 cgtggtgtcg tcgcccgggc tggggatgct gatggtggac caggggccaa gcagcggaac
     2101 gtgctgcagg atcgggtggg cgaacaccgc cggatgcgcg atgccccatc gccaggcgtc
     2161 cggatcggcg ccgaaccggg cagacagcgc gtttaccgcg ctgtccagcg acgattgcaa
     2221 cagcgccgaa cagtcaccgt tgcaccagtg cgcgccggac ggtgacagca cataggcgac
     2281 gaagtcgggc tgcggcccgg aatcacgcag cggaatgttg tgctgcttca gcaccgcctc
     2341 gttgaaccgg tccatccagg cgttgaagat cagcggctgc ggcaggtcca tcgccatgct
     2401 gccgtcccag tcgcgcagca gcgccagcgc cttgccgctc gccccgcccg gctgcgacgg
     2461 cttcaccttc agcaaggcgg gcagcacctg ttgggcgaag gtggagcgta cgtccacctg
     2521 catccgggca aaatcctccg gcgtgaacgg gccaccgctg gccagcatct gcttgatccg
     2581 cttcgcccgc caatcgccga accagtcgcg gccgaggaac accgggaagt ccggcggggc
     2641 aatccgctca ttggcgttga ccagcacgcc gctggccgga tcaacgtact gcggcagctg
     2701 gtcgcccgac gcccagccgg tccagtcata cgtgccgtcg ccgctcaccg gggccgcacc
     2761 gtcacccgcc ttgcggatcg gcacccgccc ggtgacgaac aggccgatat ggtcccggtc
     2821 ggcgaccagc agattctgca ccggggacgt gatcagcgcg gcggccttgc cggcttccgc
     2881 caccgacccg gcgctatcca gcgcgaacag cccctcggcc gcggtgtccc cgggggcgag
     2941 gttggccatc gccaccgcca ggatcggctg cttgccgggc agcaggtcgc tgatcaccgg
     3001 accatgccgg gtggaccgca ccttcagcat cacgtcgggc tggccgcgca ccttgatccg
     3061 ctcctcgcgg atgtggaacg gtcgcggccc gtccggcgtc tggtactcgc cagggccggc
     3121 gggctgctcg atgaagatgt cctggacgtc ggcgccggtg gtggtgaagg tccaggcgat
     3181 gtggcggttg cggcccagga tgatgcccgg cacgcccggt ccggtggcgc cgaccagcgt
     3241 gtggtccggc gtgtcgatcc gcgccaggta ccagatgccg gggaagccga actgcagatg
     3301 cggatcgccg gccagcagcg gcgcgccggt cttgctgtgc tcgccg
//
LOCUS       DASSZH010000604         2078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1429162, whole genome shotgun sequence.
ACCESSION   DASSZH010000604 DASSZH010000000
VERSION     DASSZH010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429162"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VFN42_13080"
     CDS             <1..497
                     /locus_tag="VFN42_13080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8997595.1"
                     /translation="RIMIAIALAGRPKLLIADEPTTALDVTTQAEILDLLKQLQQELG
                     MAMILITHDMGVAAECADRIAVMYAGRIVEEGDTAKVFANPRMPYTNGLLQSVVSFDT
                     PPRSLLHTIGGHAPSLVDMPQGCRFHTRCAKATAQCELDVPALDGGNGAHSWACWSPL
                     PEVA"
     gene            complement(693..998)
                     /locus_tag="VFN42_13085"
     CDS             complement(693..998)
                     /locus_tag="VFN42_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HET8997596.1"
                     /translation="MIFARQIPSPTPGEVLLLEFVRPMGLTQNGLARAIGVPPRRINE
                     IVLGKRSITADTDLRLARYFGVSEGFWLGLQADHDLLQRRREIDADLTRIAPHRRAA"
     gene            1181..1966
                     /locus_tag="VFN42_13090"
     CDS             1181..1966
                     /locus_tag="VFN42_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997597.1"
                     /translation="MNPARAIPGPTLLPDPPQDHHTSPLTPPTAELLVLLQILLVYAR
                     HLRDTLARLAQRSVAHLARGFGSAQAGFVLTALTRGLLRAIALEHVLLAQAGGQPGPP
                     AASKPRTRRPRPAQPNAQQTASQQPAGSHPAPEAADRLPILPTLRQLQAAIRRHPIDS
                     AIDDITRDLGVAFSLCEARFGNALRDAMAAYRGIVCGMSDEFPRQDAPAAAPPDQPTA
                     LHVPARKHGAIRYTVGFLRAEPGCAFPFTPASAAAAKAGAQSP"
BASE COUNT          333 a          752 c          656 g          337 t
ORIGIN      
        1 agcggatcat gatcgccatc gcgctggccg gccgtccgaa actgctgatc gccgatgagc
       61 ccaccaccgc gctggacgtg acgacgcagg cggagattct cgacctgctg aagcagcttc
      121 agcaggagtt ggggatggcg atgattctga tcacgcacga catgggcgtg gccgctgaat
      181 gtgccgaccg gatcgcggtg atgtatgccg gacgcatcgt cgaggaaggc gatacggcga
      241 aggtcttcgc caatccgcgc atgccctaca ccaacgggct gctgcaatcg gtggtcagtt
      301 tcgacacgcc gccgcggtca ttgctgcaca cgatcggcgg ccatgcgccg agcctggtgg
      361 atatgccgca gggctgccgg ttccacacgc gctgtgcgaa ggcgaccgcg cagtgcgagc
      421 tggatgttcc ggcgctggac ggcggcaacg gcgcgcattc ctgggcttgc tggtcgccgt
      481 tgcctgaagt tgcgtgagcg gggttcggca cggcgtgagg tccaccccac tgcctgacat
      541 ttgcacgtcg gggtccgcta tgggtcggaa gcggatccac cgcagcatgc tccccgtcgc
      601 ccgctgcgag ggttgccgcc cggacgcacc cggatcaccg agacattcgc gcgggcaagg
      661 gcgcggggcc gaagccactg caatgcgtcc attcatgcag cccgccgatg tggcgcaata
      721 cgcgtgaggt cagcatcgat ttcccgccgc cgctgcaaca ggtcatggtc ggcctggagg
      781 ccgagccaga acccctccga gacgccaaaa taccgtgcca gtcgcaggtc ggtatccgcg
      841 gtgatcgatc gtttgcccag cacgatctcg ttaatgcgcc gcggcggcac cccgattgct
      901 cgggcgaggc cgttttgcgt caggcccatt ggccggacga actccagcag caggacctct
      961 cctggagtgg ggctgggaat ttgccttgcg aagatcatgc cggaccataa cgcgtcgcgt
     1021 tatggcacgc gagccgcaat ggctcgggga acacggcctg gtagcgcctc gctgagtgcc
     1081 atccggctca gggaccggtc agtaagcggc gtcagccacg ctaccatccc aggccacaag
     1141 cctcttgaca tttcttacta actgggttag catgccaatc atgaacccag cacgcgccat
     1201 ccccggccca accctccttc ccgatccgcc ccaggatcac cacaccagcc ccctgacccc
     1261 gccgacagcc gagctgctgg ttctgctgca aatcctactg gtttatgccc gccacttgcg
     1321 cgacacgctc gcgcggctgg cacagcgcag cgtcgcacac ctcgcccgcg gcttcggctc
     1381 ggcgcaggct ggcttcgtcc ttaccgccct cacccgcggc ctgctgcggg ccatcgcgct
     1441 ggagcatgtg ctgctggcgc aggccggcgg ccagcccggc ccgcctgccg ctagcaagcc
     1501 ccgcacgcgc cggccgcgcc cggcgcagcc gaacgcacag caaaccgcca gccagcagcc
     1561 cgccggatcg catccggcgc cggaggcggc ggatcggctc cccatcctgc cgacactgcg
     1621 gcagttgcag gcggccatcc gccgccaccc gatcgactcc gccatcgacg acatcacccg
     1681 cgacctcggc gtcgcgttca gcctgtgcga ggcccgcttc ggcaacgcac tgcgcgacgc
     1741 gatggccgct taccgcggca tcgtctgtgg catgtccgat gaatttcccc ggcaggacgc
     1801 gcccgccgcc gccccgccgg accagcccac cgccttgcac gtcccggcac ggaaacacgg
     1861 tgccattcgc tacaccgtgg ggttccttcg cgcagaaccc ggctgtgcct tccccttcac
     1921 acctgcatcc gccgcggcgg cgaaagcggg ggcgcaatca ccctgagtct gcctggcgtt
     1981 ttcattcctt gctcgaaacg cggggccggc tcgccccggc gtggggattc gtgcccctgc
     2041 catccaagac tgggtgttgc ggatgcgcgc ctgcatga
//
LOCUS       DASSZH010000605         2616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_65967, whole genome shotgun sequence.
ACCESSION   DASSZH010000605 DASSZH010000000
VERSION     DASSZH010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65967"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..457)
                     /locus_tag="VFN42_13095"
     CDS             complement(137..457)
                     /locus_tag="VFN42_13095"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET8997598.1"
                     /translation="MSHQPDSIVRIDLHGALDIRTIGGLHTELMQALSLHTTVHINSS
                     NLESADISMIQLLIAARRSAQASGREILLHSPPAPALENVLSRGGFVQAEGEAERDPF
                     WSGG"
     gene            complement(807..1973)
                     /locus_tag="VFN42_13100"
     CDS             complement(807..1973)
                     /locus_tag="VFN42_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HET8997599.1"
                     /translation="MAGDGLSGGDRFGADIAARTDTYFNRTRKIVSRFGDKRVTYAVF
                     LRRPVISAPRLMTEWLQAVATDRQTRVEIDVMHPEGSWVGAGEPIVYLTGSLMQLSDL
                     ETILLQRIGPCCVAAHNAYQMSMALPDAAFLAMEARHCAGGEMQEMMAYAASVGSAAA
                     KQEGARGFIGNANDGTSHWFGASRGSGTMPHSLIGYAGSTLRAAEMFRETFPDEPMTV
                     LVDYFAREITDALAVCAHFADLAASGALSFRLDTHGGRFVEGLDPAESYAVLERHAPG
                     AIRRYRSETELRYLVGTGVSAAAVWRLREALDAGGYPKVRIVVSSGFGVDKCRVMADA
                     KAPIDVVGTGSFIPDSWHETYATADIVEYDGLPMVKVGREFLLRRNGARRKTGG"
     gene            complement(1978..>2616)
                     /locus_tag="VFN42_13105"
     CDS             complement(1978..>2616)
                     /locus_tag="VFN42_13105"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET8997600.1"
                     /translation="DSTTGDWYGCSAHFLWIGDRTRQVNGAHVEFLRGLKNPLGMKVG
                     PTQEPDDLLRILDILNPDNEAGRITLISRMGADKVGARLPALLRAVQRSGQKVVWLCD
                     PMHGNTISTVAKLKTRNFDSILSEVRGFFDIHAAEGTWAGGVHVEMTGQDVTECTGGA
                     HRLSEADLGERYETFCDPRLNAEQSLELAFLVAEELKQRRSPNGLLPAVAAQ"
BASE COUNT          432 a          890 c          829 g          465 t
ORIGIN      
        1 ccgccgctgc gtgccttcgc cagtccctcc gcaccgttgc cggcttgcac caccccgtaa
       61 ccgccgccgg tgagggtcag cttcaccatt tgcagaacgc tggcggaatc gtcgacgctg
      121 aggatcgtct tggccatcag ccacctgacc agaaaggatc gcgttcggcc tcgccttcgg
      181 cttgcacgaa gccgccgcgg cttaggacat tttccaatgc gggggccggt ggcgaatgca
      241 acaggatctc cctgcccgat gcttgcgcgc tgcgccgagc cgcgatcagt agctggatca
      301 tgctgatgtc ggccgattcg agattgctgc tgttgatgtg tactgtggta tgcagcgaca
      361 gagcttgcat cagttcagtg tgcaatccgc cgatcgtgcg gatgtccagt gcgccgtgca
      421 aatcgattcg cacgattgag tcgggctggt gggacaaccg atcctcctct gcgctgctga
      481 ctggccggtt ctggcacgca gggattaaca aagcgtgtgc ggcgcaaaga tttcgacgcg
      541 ccgttccgcg gggcatgcgg aacggccggc cgtgcctgga ggcagcggat ccgcttgatg
      601 acaggtttgc gccacaatgc gtcttggccg gcgaatgcgg cccagtctga agagcagacc
      661 cacccatttg gcgcaggcgc gccatccacg actggctgcg gagagcgcca ctgcagcaag
      721 tggcttgcag cgccaagtac cgtcggcgat ccgccggtga cgggcctgta acccgccgcg
      781 acgtcgccgc cgtcagccct ccgcgcttac ccgcctgtct tgcgccgagc cccgttgcgg
      841 cggagcagga attcgcggcc gaccttcacc attggcaggc cgtcatactc gacgatatcg
      901 gccgtcgcgt aggtttcgtg ccaggaatcc ggaatgaagc tgccggttcc gaccacgtcg
      961 atcggcgcct tggcatccgc catcacccgg cacttatcca cgccgaatcc ggacgacact
     1021 acgatccgca ccttcggata tcccccggca tccagcgctt cccgcaggcg ccataccgcg
     1081 gcggcggaca caccagtgcc gaccaggtac cgcaattccg tttcgcttcg atagcgccgg
     1141 atcgcgccgg gggcgtgacg ttccagcacg gcgtagctct cggccggatc cagcccttcc
     1201 acgaagcggc cgccatgcgt atccagccgg aacgacagcg cgccggatgc ggccagatca
     1261 gcgaagtgag cgcataccgc cagcgcatcg gtgatctcgc gcgcaaagta atctaccagc
     1321 acggtcatcg gctcgtcggg gaaagtctcg cggaacatct ccgccgcgcg cagcgtcgat
     1381 cctgcgtagc cgatcaagga gtgcggcatg gtgcctgagc cgcgcgatgc accgaaccag
     1441 tgggacgtgc cgtcattggc attgccgatg aagccgcgcg caccttcctg cttcgctgcc
     1501 gcgctgccga ccgaggcggc gtaggccatc atctcctgca tctcgccgcc ggcgcagtgc
     1561 cgcgcctcca tggccaggaa ggccgcatcc ggcaaagcca tgctcatctg gtaggcgttg
     1621 tgggcggcga cgcagcaggg gccgatcctc tgcagcagga tggtttccag gtcagacagc
     1681 tgcatcaggc tgccggtcag atacacgatc ggttcgcccg cgccgaccca gctgccctcg
     1741 ggatgcatca cgtcgatttc cacccgtgtc tgccgatcgg tggccaccgc ctgcagccat
     1801 tccgtcatca ggcgcggtgc ggagatcacc ggacggcgca ggaacaccgc gtaggtgacg
     1861 cgcttgtcgc cgaagcgcga cacgatcttg cgcgtgcggt taaaataagt gtcggtccgc
     1921 gcggcgatgt ccgcgccgaa cctgtccccg ccggagagac cgtcgcccgc catggaacta
     1981 ctgcgcggcg acggccggca acaggccgtt cgggctacgg cgctgtttca gctcttcggc
     2041 caccaggaag gccagttcca gcgactgctc cgcgttcagc cgggggtcgc agaaggtttc
     2101 atagcgctct cccaggtcgg cttcgctgag ccggtgtgcg ccacccgtgc attcggtgac
     2161 gtcctgcccg gtcatttcca catgcacacc gcccgcccag gtgccctctg ccgcgtggat
     2221 atcgaagaag ccgcgcactt ccgacagaat ggagtcgaaa ttgcgggtct tcagcttggc
     2281 gacggtggag atggtgttgc cgtgcatcgg gtcgcacagc cacaccacct tttggccgga
     2341 gcgttgcacc gcccgcagca gcgccggcag cctggcaccc accttgtcgg cgcccatgcg
     2401 gctgatcagc gtaatgcggc ctgcttcgtt gtccgggttc aggatgtcca ggatgcgcag
     2461 caggtcgtcg ggctcctgcg tcgggccgac cttcatgccc agcgggttct tcaacccgcg
     2521 caggaactcc acatgcgcgc cgttgacctg gcgggtgcgg tcgccgatcc acaggaaatg
     2581 cgcgctgcag ccgtaccagt cgccggtggt cgaatc
//
LOCUS       DASSZH010000606         6666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1446529, whole genome shotgun sequence.
ACCESSION   DASSZH010000606 DASSZH010000000
VERSION     DASSZH010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6666
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446529"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="VFN42_13110"
     CDS             <1..851
                     /locus_tag="VFN42_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014104779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET8997601.1"
                     /translation="AEAGSFTHAGDTLNLSQSAVSRQISALEEALQVPLFHRHARGLI
                     LTEQGEALNRTVREVFAKLAITEALLTESKDKPAGRLKVTTTVGFGSTWLAPRLHGFL
                     ETYPDVSLSLLLDDADLDLAMREADVAIRMHQPKQPDLVQRHLMSIQWHVCASPDYLK
                     EYGVPQRPEDLDAHKLILFGEYHPPVGDINWLAEAGRRPGSPRRALLEVNSTQAVLLA
                     IRSGVGIGALPDYVVNENPDLVRVLPELKAPKVDVFFVYPEELRNSKRVAVFRDFLLA
                     RLAEMH"
     gene            complement(1203..2258)
                     /locus_tag="VFN42_13115"
     CDS             complement(1203..2258)
                     /locus_tag="VFN42_13115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET8997602.1"
                     /translation="MRVSTVLPVADWRECGPAARQAEQDGFDSVQSNELRHDPFAPLA
                     FAALATERVDLVTSVAIAFPRSPMIVANQAWDLHRHSRGRFVVGLGSQVKAHNERRFS
                     VPWIAPAARLGEYVEALRAIFRCWEYGEKLDFRGKFYSFTLMTPEFSPGSQGLPLPPV
                     AMAAVGPVMLKTAARVADSVRLHGFATRLYLEHVVRPLLAEELRSAGKSFTHFEVTGG
                     GFVATGPDEAAVKAAAEKLRYRVAFYGSTPAYRPVFELHGLGELGSRLTELSKQGAWD
                     RMAAQVSDEVLDLFVARAPYEGIAEAIERRFGGVVDSVSIEFVPGTPAAVRRQVVTAI
                     QRIPGQFQGFTTQSAAS"
     gene            2348..3109
                     /locus_tag="VFN42_13120"
     CDS             2348..3109
                     /locus_tag="VFN42_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8997603.1"
                     /translation="MAELTGKVVLVTGASRGIGEAAALALGRAGAAVMLLARDGARAE
                     QVAQRVAQDGGKAAGMACDVSDYAAMQRAAAETRDRFGRLDVLVNNAGVIDPIASIAE
                     SDPAEWARNVQINLVGAYHAIRAVLPEMIAAGGGTIVNVSSGAAMRPLLGWSAYCSGK
                     AGLAMLTRAIALEHGSQGIRAFGFQPGTTDTDMQVIIRASGVNPVSQIPREKLTPVAH
                     PAAAIVYLCTKAADDLAGQEFSLGDTAFRSRLNLE"
     gene            3120..3893
                     /gene="dapB"
                     /locus_tag="VFN42_13125"
     CDS             3120..3893
                     /gene="dapB"
                     /locus_tag="VFN42_13125"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019084901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET8997604.1"
                     /translation="MAARIGIAGITGRMGRLLAEEVRAAGAMVSGGIDLPGSMAELPA
                     GAERFPDAGALAAASDVVLDFTNAAAAQTHASALAKAGAAWVLGTSGLSAADEAAVAA
                     AAQRIPVVYAANFSAGVNLVLALAERMAAALPAEQYDAEIVEMHHRQKVDAPSGTAVG
                     MGRAVAKGRGVRLEDVMESGRDGHTGARKTGAIGFAALRGGQVVGEHTLIFAAGTEHI
                     ALTHRAFDRRAFATGAVRAALWVQGRAPGLYSMMDVLGM"
     gene            complement(3907..5301)
                     /locus_tag="VFN42_13130"
     CDS             complement(3907..5301)
                     /locus_tag="VFN42_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase"
                     /protein_id="HET8997605.1"
                     /translation="MSDAAATSLLDRAIRRITTAWRDMAASVAGEEDESLEAQMRTCL
                     AGRGGEVSGRNRAAKLAQTYQSLDEDGRKHFLHTLASFDSDPDAVAHAYDAVREAGDI
                     AERATAKAALRKALEPPRLKLLTQFTSIPAGPKFLVDMRAFLLRTRGDDKLLAALESD
                     LRGLLAAWFDIGFLELARIDWNSPAALLEKLVDYEAVHAIRSWRDLKNRLDYDRRCYA
                     FFHPRMPGEPLIFVEVALVKGLAGSVQGLLDEKAPVLNPQEADTAIFYSISNCQQGLA
                     GISFGNFLIKRVVGELTSEFRNLKTFATLSPIPGFRRWLDAVLAADEPALLSEEESSS
                     LMSAMPAENGAAALAAILAKRHWWKETGLRKTAEPVLMRLCARYLLVETSRGNPKRAR
                     DPVAHFHLSNGARVERLTLCGDTSDKGAKESATLMVNYLYDPGKIEDWHEDYAGEGKR
                     NASTTVRKLARGWS"
     gene            complement(5332..>6666)
                     /locus_tag="VFN42_13135"
     CDS             complement(5332..>6666)
                     /locus_tag="VFN42_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET8997606.1"
                     /translation="RAVARAVMRAFRPCRTLVLCGPGNNGGDGYVAARLLAQGGWPVA
                     LAALAAPRTGSDAAGAAARWRGPIVPFTPAEAARAALVIDAVFGAGLARDVDGVVADT
                     LRAARRVVAVDVPSGVDGATGQIRGYAPKAVLTVTFFRLKPGHLLLPGRERCGTLHLA
                     DIGLPRAALDQVRPRSFANTPCLWPLPRLDPAGHKYARGHVTVLGGPAMTGAAQLAAD
                     AARHAGAGMVTIAASGNAAVYRSGSPGLIVNEAPLGELLQDDRRQVWICGPGLGIEGA
                     RAALPALLAARRSVVADADVFTAFAGNPDALRGAAVLTPHAGEFVRAFGAPGDDRLAA
                     VRAAAVRTGAVVLLKGADTIIAAPDGRTAINASAPPWLATAGAGDVLAGVIAGLLAQG
                     MPAWEAAAAGAWLHGRAAALHGPGLIAEDLLPALTAAFDAATKPPNAAALTI"
BASE COUNT         1025 a         2263 c         2349 g         1029 t
ORIGIN      
        1 tagccgaggc tggcagcttc acgcatgccg gcgacacgct gaacctgagc caatcggccg
       61 tctcgcggca gatctcggcg ctggaggagg cgctgcaggt gccgctgttc caccgccacg
      121 cccgcggcct gatcctgacc gagcagggcg aggcgctgaa ccgcacggtg cgagaggtgt
      181 tcgccaagct ggccatcacc gaggcgctgc tgaccgaaag caaggacaag ccggccggcc
      241 ggttgaaggt gaccaccacg gtcggtttcg gctcgacctg gctggcgccg cgtctgcatg
      301 gcttcctgga aacctacccc gacgtctcgc tctcgctgct gctggacgac gctgacctgg
      361 acctggcgat gcgggaggcg gacgtggcca tccgcatgca ccagccaaaa cagcccgacc
      421 tggtgcagcg ccacctgatg agcatccagt ggcacgtctg cgcctccccc gactacctca
      481 aggaatatgg cgtgccacag cggccggaag acctggacgc ccacaagctg atcctgttcg
      541 gcgagtacca cccgccggtt ggcgatatca attggctggc cgaagccggc cggcgaccgg
      601 gcagtccgcg gcgggcgctg ctggaggtga acagcaccca ggccgtgctg ctggcgatcc
      661 gctccggcgt cggcatcggc gcgctaccgg actacgtggt gaacgagaac ccggacctgg
      721 tgcgcgtcct gccggagctg aaggcaccga aggtggacgt gttcttcgtg tacccggagg
      781 agctgcgcaa ttccaagcgc gtcgcggtgt tccgcgactt cctgctggcg cgcctggcgg
      841 agatgcactg acctcgtctc cttggcaggg cctgtgccct cggctccgca atccaggatt
      901 gtggccagga gatgacatgc gcacctagca tggcggatgc cggtatttgg cgatgcacac
      961 ctcgggaggg tgccactact gtttggtcac ccgttagggc ggcactgccg ccagtgttcc
     1021 ggcccttgtg gctggaaaag gggcctttgg tccctgcgtc tcccagacgt gaagcctccc
     1081 tgtattactt tgcccccggc ttccgccggg ggcatttttc tatgtagagg ctcccgttac
     1141 tgtcgttcgg cagtgccgga tttcacgtac ggctcacgcg gacacctctg tcctggcccc
     1201 cctcagcttg ccgcggactg cgttgtaaag ccctgaaatt ggccgggtat gcgctggatt
     1261 gccgtcacca cctggcgtcg taccgcggcc ggggtgcccg gtacgaactc gatggagacg
     1321 ctgtccacca cgccgccgaa acgccgctcg atggcttcgg cgatcccttc ataaggggcg
     1381 cgggcgacaa acagatccag cacctcatcg gagacctgag cggccatgcg gtcccaggcg
     1441 ccctgtttcg acaactcggt cagccgggag ccaagctcgc ccagcccgtg cagctcgaag
     1501 accgggcgat aggcgggggt ggagccgtag aacgccacgc gatagcgcag tttctccgcg
     1561 gcggccttga ccgcagcctc atcgggaccg gtcgccacga agccgccgcc ggtcacctcg
     1621 aaatgcgtga aagacttgcc ggcggatctg agttcctccg ccagcagcgg ccgtaccacg
     1681 tgttccaggt agaggcgggt ggcgaagcca tgcaaccgga cgctgtcggc gacgcgcgcc
     1741 gcagtcttca gcatcaccgg cccgaccgct gccatcgcca caggtggcaa gggcaggccc
     1801 tgcgagccgg gcgagaactc cggcgtcatc agggtgaagc tgtagaattt gccacgaaaa
     1861 tcgagcttct cgccgtattc ccagcagcgg aagatcgcgc gcagcgcctc gacgtactcg
     1921 ccaagacgcg cggccggggc gatccagggc acgctgaagc ggcgctcgtt gtgcgccttc
     1981 acctggctgc cgaggcccac cacgaaacgc ccgcgcgagt ggcggtgcag gtcccaggcc
     2041 tggttcgcca cgatcatcgg actgcggggg aaggcaatcg ccaccgatgt gaccaggtcc
     2101 acgcgctcgg tggccagtgc ggcgaaggcg agcggggcaa agggatcgtg ccgtagttcg
     2161 ttgctctgca cgctgtcgaa cccgtcttgt tccgcctggc gcgccgccgg tccgcattcg
     2221 cgccagtccg ccaccggcag caccgtggat acccgcatcg tctcgctccc cctggttttc
     2281 atccggcaat ttgcggtcgc cgcgtgcctc ccgtaaagcg cggatcatgg aacagggagg
     2341 agcgggcatg gccgagctga cggggaaagt ggtgctggtg accggcgcga gccgcggcat
     2401 cggcgaggca gcggccctgg cgcttggccg cgccggcgcg gcggtgatgc tgctggcgcg
     2461 cgacggtgcg cgagccgagc aggtggcgca gcgggtcgca caggatggcg gcaaggccgc
     2521 gggtatggcc tgtgacgtct cggactatgc cgcgatgcag cgcgcggcgg cggaaacacg
     2581 cgaccggttc ggccgcctgg acgtgctggt gaacaacgcc ggcgtgatcg atccgattgc
     2641 cagcatcgcc gagagcgatc cggcggagtg ggcgcgcaac gtgcagatca atctggtcgg
     2701 cgcctatcac gcgatccgcg cagtgttgcc ggagatgatc gcggccgggg gcggcaccat
     2761 tgtcaacgta tcgtccgggg cggcgatgcg gccgctgctg ggatggagcg cctattgttc
     2821 cggcaaggcc gggctggcga tgctgacccg cgccatcgcc ctggagcacg gatcgcaagg
     2881 cattcgtgcc tttggtttcc agccgggcac caccgatacc gacatgcagg tgatcatccg
     2941 cgcgtccggc gtgaatccgg taagccagat tccacgcgag aagctgactc cggtggcgca
     3001 tccggccgcg gcgatcgtgt atctgtgcac caaggcggcg gacgatctcg caggccagga
     3061 attcagcctg ggcgacacgg cattccgcag ccgcctgaac ctggaatagg gaggcacgca
     3121 tggctgcgcg catcggcatt gccggcatca ccggccggat ggggcggttg ctggcagagg
     3181 aggttcgggc ggcgggcgcc atggtcagtg gcggcatcga cctgccgggc agcatggcgg
     3241 aacttcccgc cggcgcggag cgctttccgg atgccggcgc cctggctgcg gcctcggatg
     3301 tcgtgctcga tttcaccaat gccgccgcag cgcagacgca tgcctcggcc ttggccaagg
     3361 cgggcgcggc ctgggtgttg ggcacctccg gcctgtccgc cgcggatgag gcggcagtgg
     3421 cagcggcggc gcagcgcatc ccggtggttt atgcggcgaa cttttccgcc ggcgtcaatc
     3481 tggtgctggc gttggccgaa cgcatggcgg cggccctgcc ggcggagcag tacgacgccg
     3541 agatcgtgga gatgcatcat cgccagaagg tcgatgcgcc gtccggcacc gcagtcggca
     3601 tgggacgcgc ggtggcgaag gggcgggggg taaggctgga ggacgtgatg gagagcggcc
     3661 gggacggtca taccggcgcg cgcaagaccg gggcgatcgg cttcgcggcg ctgcgcggtg
     3721 ggcaggtggt gggcgagcac acgctgatct tcgccgccgg caccgagcac atcgcgctga
     3781 cccatcgcgc gttcgaccgc cgtgccttcg cgactggcgc cgtgcgggcg gcgttatggg
     3841 tgcaggggcg ggcgcctggg ctgtattcga tgatggacgt gctggggatg tgacgcgagg
     3901 cagccgtcag ctccacccgc gggccagttt acgcaccgtc gtcgaggcgt tgcgcttgcc
     3961 ctcgccggcg tagtcttcgt gccagtcctc gatttttccg ggatcgtaca ggtagttgac
     4021 catcagcgtg gcgctttcct tggcgccctt gtccgaggtg tcgccgcata gcgtcagccg
     4081 ttccacccgg gcgccgttgg acaaatggaa atgcgccacc ggatcgcgcg cccgcttcgg
     4141 attgccgcga ctggtttcca ccagaagata gcgcgcgcag agccgcatca acaccggctc
     4201 cgcggtcttg cgcaggccag tctccttcca ccaatggcgc ttggccagga tcgcggccag
     4261 cgccgctgcg ccgttctctg ccggcatggc cgacatcagg ctcgacgatt cttcctccga
     4321 cagcagtgcg ggttcgtcgg ccgccagcac ggcgtccagc caccggcgga acccggggat
     4381 cggcgacagc gtggcgaagg tcttgagatt gcggaactcg ctggtcagct cacccaccac
     4441 ccgcttgatc aggaaattgc cgaagctgat ccccgccagg ccctgctggc agttgctgat
     4501 cgagtagaag atcgcggtat ccgcctcctg cgggttcagc accggcgcct tctcgtccag
     4561 cagcccttgc acgctgccgg ccaggccttt caccagcgcg acctccacga agatcagcgg
     4621 ctccccgggc atgcgcggat ggaagaacgc gtagcagcgg cggtcgtaat ccagccggtt
     4681 cttcaggtcg cgccaggagc ggatggcgtg taccgcctca taatccacca gcttctccag
     4741 cagcgctgcg ggagaattcc agtcgatacg cgccagctcc aggaagccga tatcgaacca
     4801 tgccgcgagc aggccgcgca ggtcggattc cagcgccgcc agcaacttgt cgtcgccgcg
     4861 cgtccgcagc aggaacgccc gcatgtccac caggaatttc ggcccggccg ggatgctggt
     4921 gaattgcgtc aacagcttca gccgcggcgg ttccagcgcc ttgcgcagtg cggccttcgc
     4981 agtggcccgc tcagcgatgt ccccggcctc gcgcaccgca tcataggcgt gtgccaccgc
     5041 gtcgggatcg gaatcgaagc tggccagcgt atgcaggaag tgctttcgcc cgtcctcatc
     5101 cagtgactgg taagtctgcg ccagcttggc ggcgcggttg cgcccgctga cctcgccacc
     5161 gcggccggcc aggcaggtac gcatctgggc ttcgaggctt tcgtcctcct cgcccgcgac
     5221 gctggccgcc atgtcgcgcc aggcggtggt gatgcggcgg atggcccggt caagcagact
     5281 ggtcgcggcg gcatcactca tggcacccgg gctctcctga aacgatccgt atcatatggt
     5341 gagggccgcg gcgttgggag gctttgttgc cgcatcaaac gcagccgtga gcgccggcag
     5401 caggtcctcc gcgatcagtc cggggccgtg cagagcggcg gcgcggccat gcagccaggc
     5461 gccggcggcc gccgcttccc acgctggcat gccttgggcc agcagcccgg cgatcacccc
     5521 agccagcacg tcaccggcgc cggccgtcgc gagccaaggc ggggcggagg cattgatcgc
     5581 agttcggccg tccggcgcag cgatgatggt gtcggcgccc ttcagcagca ccaccgcgcc
     5641 ggtgcggacg gcagcggcgc ggacggcggc cagccggtcg tcgccgggcg cgccgaacgc
     5701 ccggacaaat tcgccggcat gcggggtcag caccgcggcg ccgcgcagcg cgtcgggatt
     5761 gccggcgaag gcggtgaaca catccgcgtc cgccaccacg ctgcgccgag cggcgagcag
     5821 cgcaggcagt gcggcgcgtg cgccctcgat gcccaggccg gggccgcaga tccagacctg
     5881 gcggcggtca tcctgcaaca gctcgcccag cggggcttcg ttgacgatca ggccgggcga
     5941 accgctgcga tacaccgctg cattgcccga ggcggcgatg gtgaccatgc ccgcgccggc
     6001 atgccgcgcg gcatccgccg ccagctgcgc ggcgccggtc atcgcggggc cgccgagcac
     6061 cgtgacgtgg ccgcgggcgt atttgtggcc ggcggggtcc agtcggggca gcggccacag
     6121 gcagggggtg ttggcgaagc tccgcggtcg cacctggtcc agcgccgccc gaggcaggcc
     6181 gatatccgcg aggtgcagcg tgccgcagcg ttcgcggcct ggcagtagca ggtggcccgg
     6241 cttcaggcgg aagaaggtca cggtcagcac ggccttcggg gcatagccgc ggatctggcc
     6301 ggtggcgcca tccacgccgc tgggcacatc cacggcgacg acgcggcgtg cggcgcgcag
     6361 cgtgtcggcg acgacgccgt ccacatcgcg cgccaacccg gcaccgaaca cggcgtcgat
     6421 caccaatgcg gcgcgcgccg cctcagccgg agtgaacggg acgatggggc cgcgccagcg
     6481 cgcggcggcg ccggccgcat cggatcccgt acggggtgcg gcgagcgcag cgagcgcgac
     6541 cggccagccc ccctgtgcca gcagccgcgc ggcaacatag ccgtcccctc cgttattgcc
     6601 cgggccgcac agcaccagcg tgcggcaggg gcggaacgcc cgcatcaccg cgcgcgcgac
     6661 cgctcg
//
LOCUS       DASSZH010000607         2044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1599844, whole genome shotgun sequence.
ACCESSION   DASSZH010000607 DASSZH010000000
VERSION     DASSZH010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1599844"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VFN42_13140"
     CDS             complement(<1..753)
                     /locus_tag="VFN42_13140"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HET8997607.1"
                     /translation="MSRRLGVGHWHRVRRFGRTLRRRSHAELWFPHVPLFLMIGSLGL
                     LQIRHALGIGLLDHVTATDILAMSQSALTAVVNGAPSATAGVFLVVMGFGLLTRSRLA
                     WVIALIAAAVSAMLIWLVWAGSADDKSGLLVYNVFTLLMLLTAGGAFTRSSLTSASLF
                     ATASIVLLIAYAVLGSYLLGKQFKPPVTDLVSALYYAVVTMTTIGYGDIVPVTTEARM
                     FAISVMILGITVFATSVSALLVPMINRRMERLL"
     gene            1017..>2044
                     /locus_tag="VFN42_13145"
     CDS             1017..>2044
                     /locus_tag="VFN42_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HET8997608.1"
                     /translation="MMLQEEPVAVGRPEQGVGEQRRSSAPAFAALDLGTNNCRMLVGT
                     PAGDGFRVLDSFSRIVRLGEGLHHTGCLSDTAMDRALGALHACAARLARRPVRRLRAI
                     ATEACRRAANGAEFLARVRAETGLDIGVISAREEAELALESCTPLLAGGGRRALLFDI
                     GGGSTELAWVRLSEGRPVLIGYDSLPIGVVTLAERWGNAGFQAAGFEAMVDDVVARLA
                     PFESIHCIGHEIRDGGVSLLGTSGTVTTLAGVALNLTRYRRPLVDGVALSATDADAAL
                     SSLRTLGREGLMAHPCVGPDRVDFVLPGCAVFAAITRLWPAPRVLVADRGLREGMLLR
                     MIRGERDRG"
BASE COUNT          322 a          670 c          738 g          314 t
ORIGIN      
        1 aagcagccgt tccatccggc ggttgatcat cggcaccagc agggcgctga ccgaggtcgc
       61 gaacacggta atgcccagta tcatcaccga aatcgcgaac atgcgcgctt cggtggtcac
      121 cgggacgatg tcgccgtagc cgattgtcgt catggtgacg acggcatagt acagcgcgga
      181 aaccaggtcg gtcaccggcg gcttgaactg cttgcccagc aggtagctgc ccagcacggc
      241 gtaggcgatc aacagcacga tgctggcggt ggcgaacagc gatgcgctgg tcaggctgga
      301 gcgggtgaac gcgccgccgg cagtcagcag catcagcagg gtgaacacgt tgtagaccag
      361 caggccgctc ttgtcgtcgg ccgaaccggc ccagaccagc cagatcagca ttgcgctgac
      421 cgccgcggca atcagcgcga tcacccaggc aagccgcgac cgggtcaaca gcccgaagcc
      481 catcaccacc aggaatacgc ccgccgtcgc gctgggcgcg ccgttcacca ccgcggtcag
      541 tgcgctctgc gacatggcga ggatgtcggt cgccgtgacg tgatccagca gaccgatgcc
      601 cagcgcgtgg cgtatctgca gcaggcccag gctgccgatc atcaggaaca gcggcacatg
      661 cgggaaccac agttcggcgt ggctgcggcg gcgcagggtg cggccaaacc ggcggacgcg
      721 gtgccagtgg ccgacaccca gtcggcggga cacctcacgg gattgcctgg cggtctgttg
      781 agtggtcacg cgactgggag catccagggg tggaaaatag cgggcgagcg aaaacgaaat
      841 gtggacgtga tggttcatcc ctgccagtcc tgtgaccata tcggtccggg ttccggcgct
      901 cttgccgggt gttgcgtgcc cgatgccgcc tgcagcgagg gtgaaatctc ggccacaact
      961 gcgggacccg ggcattcgag gcttgccacc gcccggcgcg ctgggaacaa tattgtatga
     1021 tgctgcagga agaaccggtt gcggtcggcc gccccgaaca gggggtggga gagcaacggc
     1081 gatcgtctgc gcccgccttc gccgcgctcg accttggcac caacaactgc cgcatgctgg
     1141 tgggcacgcc ggccggcgac ggcttccggg tgctcgacag tttctcccgg atcgtgcgcc
     1201 tgggcgaggg gctgcaccac accggctgcc tgtcggatac cgccatggat cgcgcgctgg
     1261 gtgcgctgca cgcctgcgcc gcccggctgg cgcgccgtcc ggtccgccgc ctccgggcga
     1321 tcgccaccga ggcctgccgc cgagcggcca acggcgcgga attcctggcc cgggtccggg
     1381 cggagaccgg actggacatt ggcgtgatct ccgcccgcga ggaagcagag ctggccctgg
     1441 aatcctgcac cccgctgctg gcgggcggcg gacggcgggc tctgctgttc gatatcggcg
     1501 gcggctccac cgagcttgcc tgggtgcggc tgagcgaagg ccggccggtg ctcatcggct
     1561 acgactcact gccgatcggc gtggtgacgc tggccgagcg ctggggcaat gccgggttcc
     1621 aggcggccgg gttcgaggcg atggtggatg acgtggtcgc ccgcctggca ccgttcgagt
     1681 cgatccactg catcggccat gaaatccgcg acggcggggt gagtctgctg ggcaccagcg
     1741 gtaccgttac cacccttgcc ggggtggcgc tgaacctgac ccgctatcgc cgcccgctgg
     1801 tggatggcgt ggcgctgagc gcgaccgatg ccgacgcggc gctgagcagc ctgcgcacgc
     1861 tcggccggga agggctgatg gcgcatccct gcgtcgggcc ggaccgggtg gatttcgtgc
     1921 tgcccggctg cgcggtattc gccgcgatca cccgcctgtg gccggcgcca cgcgtcctgg
     1981 tggcggatcg cgggctgcgc gagggcatgc tgctgcgcat gatccgtggc gaacgcgatc
     2041 gtgg
//
LOCUS       DASSZH010000608         3503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1242052, whole genome shotgun sequence.
ACCESSION   DASSZH010000608 DASSZH010000000
VERSION     DASSZH010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242052"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VFN42_13150"
     CDS             complement(<1..198)
                     /locus_tag="VFN42_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997609.1"
                     /translation="MVTLRQCVILTGRYDGAASPLWADCAGRPALAWVLREFQRFGVS
                     DFLLLTDDASGAVAQAAPTAAA"
     gene            321..695
                     /locus_tag="VFN42_13155"
     CDS             321..695
                     /locus_tag="VFN42_13155"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HET8997610.1"
                     /translation="MADNPLEPDAARERRIRERAYHLWEAEGRPHGRDQEFWERAREL
                     IAIEDHPGAGQLPNPETHPDSPRETGIEEAEIQENLGEFPDRFADQGEVQQTPSAEHS
                     PRKRKAPAAPPKRAAKPRPRKT"
     gene            798..2573
                     /locus_tag="VFN42_13160"
     CDS             798..2573
                     /locus_tag="VFN42_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015441 - ABC-type beta-glucan transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015775 - beta-glucan transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan ABC transporter ATP-binding protein/
                     permease"
                     /protein_id="HET8997611.1"
                     /translation="MRFLRVYARVLAMLGRDIRVAGLLAGANLIVAALQFLDPVLFGH
                     VITLLSHSDSMAAKAFWAAGMGLLAIWAGVGLAGIFCNIFVSVQTERLSHRHRIAALG
                     RYFQHVLNLPASFHGDTQSGRLIKVMLTGTDALFGTWLVFFRDQLATFLSAVVLLPLT
                     LLLNWRLALVLIVLVVIFVLVTALVVRKTEAGQRRVEGYNSALAGTAQDALTNVMVVQ
                     SFTRLAAEVKQFGEIAEALIRNQFPVLNWWALVNVMTRASSTLAVITIVTVGAWLHMH
                     GQASVGEIVSFMGIAMLLIGRLETAASFVSSLFFKMPVLEQYFDVLDSQSSVPEQPDA
                     RPLWAPRGEVRFDQVSFGYTPGRDVLSELDFVAKPGRSVALVGHTGAGKTTAMALLQR
                     LWDPSAGRITIDGQDLRKVSLDSLRSNIGVVFQESLLFNRSIRDNLLVGRPDATQEEV
                     EAACRMADAHEFIIRQPQGYDTMVGERGTQLSGGQRQRLAIARALLKDPRILILDEAT
                     SALDAATEARVGQALRRLMAGRTTFIIAHRLSTVRDADEILVFDNGRIVERGSFAELL
                     ARRGRFAALVETQLAPAAPALMAAE"
     gene            2663..2836
                     /locus_tag="VFN42_13165"
     CDS             2663..2836
                     /locus_tag="VFN42_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010506679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HET8997612.1"
                     /translation="MLRLALFFLVVAIIAAVFGFGGIAAASAEIAKILFFIAIVIFVA
                     LLVAGLMAGSTLW"
     gene            2988..3422
                     /locus_tag="VFN42_13170"
     CDS             2988..3422
                     /locus_tag="VFN42_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET8997613.1"
                     /translation="MTVAAILKHKGFQITAVDPTATIAQVAEVLAQRRIGAVLVLDRA
                     EQLLGIVSERDIVRSLAANGARTLEMTAGQLMTRTLQVAHPEMGETEVMAMMTAGRFR
                     HLPVTDHGVLVGLISIGDVVKARIMQQEAEVDSLRAYVAGAA"
BASE COUNT          550 a         1178 c         1194 g          581 t
ORIGIN      
        1 ggcggcggcg gtgggcgccg cctgcgccac ggcgccggac gcgtcatcgg tcagcagcag
       61 gaagtcggac acgccgaaac gctggaactc gcgcaatacc caggcgagcg ccggccggcc
      121 ggcgcaatcc gcccacagcg gactcgccgc gccgtcatac cggccggtca gaatcacgca
      181 ttgccgcagc gttaccacgc cctttgcctc cacccagcca gcatctcaca aacttgattt
      241 cgcgcccggc tcctatcgcc ttccgccggg tttagccacc agttacctcc cgcagaccgc
      301 aaccaggaga tttgtgccac atggcagaca acccgctgga accggatgcc gcgcgtgagc
      361 gccggattcg cgaacgcgct tatcacctgt gggaagcgga gggtcgccca cacggtcgtg
      421 atcaggaatt ctgggagcgg gcgcgcgagc tgatcgcgat cgaggaccac cccggcgccg
      481 gccagttgcc caacccggaa acccatccgg attcgccgcg cgaaaccggg atcgaggaag
      541 cggaaatcca ggaaaacctt ggcgaattcc ccgatcgctt cgccgatcag ggcgaggtgc
      601 agcagacgcc ctcggcggag cacagcccgc gcaagcgcaa ggcgcctgcc gcgccaccga
      661 agcgcgcagc caagccgcgt ccgcgcaaga cgtaacgaga agccaagccg cgtccccgct
      721 tctgccctgt tgctgccaca cgccggcgcg aactcggtgg aaccaagaga gacagccatc
      781 tccccgggga cgaatccatg cgcttcctgc gtgtctatgc ccgtgtgctc gcgatgctcg
      841 gccgcgacat ccgcgtcgcc ggcctgctgg ccggtgccaa cctgatcgtc gccgccttgc
      901 agttcctcga tccggtgctg ttcggtcacg tgattaccct gctgtcccac tcggacagca
      961 tggccgccaa agcgttctgg gccgccggca tgggtctgct ggccatatgg gccggtgtcg
     1021 gcctggccgg catcttctgc aacatctttg tctcggtgca gaccgaacgg ctgtcgcacc
     1081 gccaccgcat cgcggcgttg ggccgctact tccagcacgt gctgaacctg ccggcgtcgt
     1141 tccacggcga cacgcagtcc ggccggctga tcaaggtgat gctcaccggc acggacgcgc
     1201 tgttcggcac ttggctggtg ttcttccgtg accagctcgc caccttcctg tccgccgtcg
     1261 tcctgctgcc gctgacgctg ctgctgaact ggcggctggc gctggtgctg atcgtgctgg
     1321 tggtgatctt cgtgctggtc accgccctgg tggtgcgcaa gacggaagcc ggccagcgcc
     1381 gggtggaggg ctacaactcc gcgctcgccg gcaccgcgca ggacgcgctg accaacgtga
     1441 tggtggtgca gtccttcacc cggctggcgg cggaagtgaa gcagttcggc gagatcgccg
     1501 aggcgctgat ccgcaaccag ttcccggtgc tcaactggtg ggcgctggtc aacgtgatga
     1561 cgcgcgcgtc cagcacgctg gcggtgatca ccatcgtgac ggtcggcgcc tggctgcaca
     1621 tgcacgggca ggccagcgtg ggcgagatcg tcagcttcat gggcatcgcc atgctgctga
     1681 tcggccggct ggagaccgcc gcatccttcg tctcctcgct gttcttcaag atgccggtgc
     1741 tggagcagta tttcgacgtg ctggattcgc agtccagcgt gccggagcag ccggatgcgc
     1801 gtccgctctg ggcgccgcgc ggcgaggtgc ggttcgacca ggtgtcgttc ggctacacgc
     1861 cgggccgcga cgtgctgtcg gagctggact tcgtcgcgaa gcccggccgc tcggtggcgc
     1921 tggtcggcca taccggggcc ggcaagacca cggcgatggc gctgctgcag cggctgtggg
     1981 accccagcgc aggccgcatc accatcgacg ggcaggacct gcgcaaggtc agcctggaca
     2041 gcctgcgcag caatatcggc gtggtgttcc aggagagtct gctgttcaac cgctcgatcc
     2101 gcgacaacct gctggtgggc cgccccgatg cgacgcagga ggaggtggag gccgcctgcc
     2161 gcatggccga cgcccacgag ttcatcatcc gccagccgca gggctacgac accatggtgg
     2221 gcgagcgcgg cacccagctg tccggcgggc agcgccagcg cctggccatc gcccgcgcgc
     2281 tgctgaagga cccgcgcatc ctgatcctgg acgaggcgac cagcgcgctg gatgccgcca
     2341 cggaggctcg ggtgggtcag gcgctgcgcc ggctgatggc gggtcgcacc accttcatca
     2401 tcgcccaccg cctgtccacc gtgcgcgacg ccgacgagat cctggtgttc gacaacggcc
     2461 gcatcgtgga gcgcggcagc ttcgccgagc tgctggcgcg gcggggccgt ttcgcggcgc
     2521 tggtggagac gcagcttgcg ccggcggcgc cggccctgat ggcggcagag tgatggttgt
     2581 caaggccacg tgaagaccgg aacgctagtg cacctactat gttccaggct gtcggcaaca
     2641 gaccgcagga gcggaatacc tcatgttacg actggcattg ttcttccttg tcgtcgcgat
     2701 cattgccgcg gttttcggct tcggcggtat tgctgcggct tccgccgaga tcgccaagat
     2761 cctgttcttc atcgcgatcg tcatcttcgt cgcgctgctg gtggcggggc tgatggcagg
     2821 aagtacctta tggtagcgta atttgctgca atgcacggat aagttttgca ttgcaggggc
     2881 cggatcgcgc atcatgcctg cgcaagcagc ggcgggtgcg cgatccggcc cggctttctg
     2941 atccgcctgg caccggaggc ggcccgcccg ttcagggagc aggggccatg acggttgcgg
     3001 caatcctcaa gcacaagggc ttccagatca ccgcggtgga tcccaccgcg acgatcgcgc
     3061 aggtggcgga ggtgctggcg cagcgccgga tcggtgcggt gctggtgctg gaccgggcgg
     3121 aacagctgct gggcattgtc agcgagcgcg acatcgtccg cagcctggcg gccaacggcg
     3181 cccgcacgct ggagatgacc gcgggccagt tgatgacgcg cacgctgcag gtggcgcatc
     3241 ccgaaatggg cgagaccgag gtcatggcga tgatgaccgc cggccgcttc cgccacctgc
     3301 cggtgacgga tcatggcgtg ctggtggggc tgatcagcat cggcgacgtg gtgaaggccc
     3361 gtatcatgca gcaggaggcc gaggtggaca gcctgcgcgc ctacgtcgcc ggcgcggcat
     3421 agaaacaggc cgcccggtca tggccgggct tgtcccggcc atccatgccg tctccatcca
     3481 gcggagacgg ccagccatga tcg
//
LOCUS       DASSZH010000609         5565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1446562, whole genome shotgun sequence.
ACCESSION   DASSZH010000609 DASSZH010000000
VERSION     DASSZH010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446562"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VFN42_13175"
     CDS             <1..210
                     /locus_tag="VFN42_13175"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424673.1"
                     /note="Catalyzes the reversible phosphorolysis of
                     5'-deoxy-5'- methylthioadenosine (MTA) to adenine and
                     5-methylthio-D-ribose-1- phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HET8997614.1"
                     /translation="DAVVKVLLDNADKARALVQQVVPEIGRNRTLCAAKCDRGLDNAV
                     ITAAHMRDPELLAKLDAVAGRVLRG"
     gene            complement(219..1142)
                     /locus_tag="VFN42_13180"
     CDS             complement(219..1142)
                     /locus_tag="VFN42_13180"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HET8997615.1"
                     /translation="MDPGRAVRQHCSMIVVFGSINLDLIFALPSIPRAGETVLGADTR
                     IEPGGKGANQAVAAARDGARVVIAGAVGRDALAPGALRLLREVGVDLSRVAETDAATG
                     CAAITVDPAGNNAIAVGSGANLLARADQVEDALLSPATTLLLQMEVPAAETAALIRRA
                     RGRGARIVLNLAPAGSLPEDALRALDVLLVNETEAAWLCHQLGCGSTAADLHAALGGV
                     DAVRTLGGDGIEAATKAGMWRIPARPIRAVDTTAAGDCFAGVLAAALDRGTDMAAALL
                     RANVAAALCCTRAGSQGSLPTAAETDAALGK"
     gene            1259..2410
                     /locus_tag="VFN42_13185"
     CDS             1259..2410
                     /locus_tag="VFN42_13185"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET8997616.1"
                     /translation="MDITRIPGSRIVEVSRVAFATPDVEFLCFGESDQPTPDSARQAA
                     VAALDAGMTRYSDVRGMPALRTALASYLTGLHARPVDESRIQVTASGMTAVTVALSAV
                     VRPGDRVVVHSPIWPNIPHALALRGARTDTIDLQAQPDGSFRLDLNQLDAMLDGARAF
                     VLNSPNNPTGWTATQEELAAILEISRRRGVWLISDEVYSRLVYGALTAAPSLLDVAAP
                     DDRVIVCNSFSKTWVMTGWRLGWLVLPDGTRDAVSDIVEVIHSGVAPFIQQAGVAAID
                     DAEALQRFRAYCGAGRALASQALSGINGIRYTPPAGAFYAFVGVEGLTDSLDLALKLV
                     TKHRVAVAPGIAFGAAGEGSLRICFAQSPERMERAMQRLRDGLRAELAA"
     gene            2407..3081
                     /locus_tag="VFN42_13190"
     CDS             2407..3081
                     /locus_tag="VFN42_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997617.1"
                     /translation="MSGTTSPRCALCGSRARTPFRAPTPELAPDLDMRPGEPAWETVQ
                     DWLQTCDRCGAVAPDLAALPETAKAVVQSAAYRDLADEKSEETLPFRRWALLCRAAGD
                     SQAATEAMLMAAWAADDAVSMLEASRLRLTVAEAWGETEDQATALWRLDVLRRSSHFA
                     AADAWANTLAARPLDRAGKAILAFQRDRIAQRDIGRHLVTSALPPGVEHETLKRPGFW
                     SWLFRE"
     gene            complement(3227..3922)
                     /locus_tag="VFN42_13195"
     CDS             complement(3227..3922)
                     /locus_tag="VFN42_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HET8997618.1"
                     /translation="MSDEAPGWVARKLLRAARVGTLATSAQGQPFASLVTPATAPDLA
                     VLLLLSDLSEHTRHLRAEPRCSVLVRGAAEAANPQTAPRVTVTGVAERLDDPALKARY
                     VALHPYSALYADFGDFAIWRIRPLGGLFVGGFARASRLRAADLAPDAAAVSAVAAAEP
                     EIIAHCNADHADAMAAIGGAGEAWRMVAADVDGCDLAAGERVVRVPWSAPVSDPGGVR
                     RELIRLARAARGD"
     gene            4183..>5565
                     /locus_tag="VFN42_13200"
     CDS             4183..>5565
                     /locus_tag="VFN42_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HET8997619.1"
                     /translation="MSLAYAVIIACGVLALIYGLITSRQVLAADAGTARMQEISGAVQ
                     EGARAYLNRQYTTIAIVGVVIMIILGVILGLHVAIGFLIGAVLSGSAGYIGMNVSVRA
                     NVRTAQASKQGLAAGLDIAFKSGAITGLLVVGLGLLGISIYYLILRSTMTGTDIRPVL
                     EAMVALGFGGSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNVG
                     DNVGDCAGMAADLFETYAVTIVATMLLSAIYFTGSVRDWLLLLPLGIGGICILTSVIG
                     TYFVKLGADNGIMKALYKGLLVTGALSVIGIAFIIAWFVGFGAPLAVTTGGSITGMSI
                     FGCALVGLAVTGLIVWITEYYTATEYRPVRSISQASTTGHGTNVIQGLAISMEATALP
                     VLVICVGIIVASIIAGLFGIAIAATTMLSLAGMIVALDAYGPVTDNAGGIAEMAGLPK
                     EVRKSTDALDA"
BASE COUNT          828 a         2025 c         1863 g          849 t
ORIGIN      
        1 gacgcggtgg tgaaggtgct gctggacaat gccgacaagg cgcgtgcgct ggtgcagcag
       61 gtcgtcccgg agatcggccg caaccgaacc ctgtgcgcgg cgaaatgcga ccgcggcctg
      121 gacaacgcgg tgatcaccgc agcgcatatg cgcgacccgg aattgctggc caagctggac
      181 gcggtcgcgg gacgcgtgtt gcggggatag gcctgcggct actttccaag ggccgcgtcg
      241 gtctccgccg ccgtgggcag gctgccctgg ctgccggcgc gggtgcagca cagcgccgcc
      301 gcgacgttcg cccgcagcaa cgccgccgcc atgtcagtcc cgcggtccaa tgcggctgcc
      361 aggacgccgg cgaagcaatc gcccgctgcg gtggtgtcca ccgcacggat cggcctggcc
      421 gggatacgcc acatccccgc cttggtggcg gcttcgatcc cgtccccgcc caatgtccgc
      481 accgcgtcca ccccgccgag cgcggcgtgc agatcagcgg cggtgctgcc gcagccgagc
      541 tggtggcaca accaggcggc ttcggtctcg ttcaccagca gcacgtccaa cgcgcgcaac
      601 gcgtcttccg gcaagctgcc ggccggcgcc aggttcagca cgatgcgcgc gccacgtccg
      661 cgcgcccgtc ggatcagcgc ggcggtctcc gccgccggca cctccatctg cagcagcagc
      721 gtggtggcgg gcgacagcag cgcgtcctcc acctgatccg cgcgtgccag caggttggcg
      781 ccggagccga ccgcgatggc attgttgccg gctggatcaa cggtgatcgc ggcgcatccg
      841 gtcgcggcat cggtctccgc cacccgcgac aggtccacgc cgacctcccg cagcaggcgg
      901 agcgcgccag gggccaacgc gtcgcgcccg accgcgcccg cgatcaccac ccgggcgccg
      961 tcgcgcgcgg cagccacggc ctggttggcg cccttcccgc ccggctcgat ccgggtatcg
     1021 gctcccagca cggtctctcc cgcgcgggga atcgacggca gggcgaagat caggtcgagg
     1081 ttgatggagc cgaacacgac gatcatggaa cagtgttgcc gcacggcacg gcccgggtcc
     1141 atgccggttt atctgctgcg gcgcagcgtg tatccgggct tgcgcgaggc gtcctgaccc
     1201 cggccagtgc tgccccggca acctcgaatt tctcccccgc aacaccaacc tacgccccat
     1261 ggacatcacc cgcatcccag gctcccgtat cgtcgaagtg agccgggtgg cctttgccac
     1321 tccggacgtc gaattcctgt gcttcggcga atccgaccag ccaacgccgg actccgcccg
     1381 ccaggccgca gtggcggcgc tggacgccgg catgacccgc tactccgacg tgcgcggcat
     1441 gccagcgctg cgcaccgcac tggccagcta tctgaccggc ctgcacgcca ggccggtgga
     1501 tgaaagccgc atccaggtca cagcgtccgg catgaccgcc gtcaccgtgg cactctcggc
     1561 cgtagtgcgg cccggcgacc gcgtggtggt gcacagcccg atctggccga acatcccgca
     1621 cgcgctggca ctgcgcggtg cccgcaccga cacgatcgac ctccaggcgc agccggacgg
     1681 cagcttccgc ctggacctga accagctgga cgccatgctg gacggcgccc gcgccttcgt
     1741 cctgaactcc cccaacaacc ccaccggctg gaccgcaacg caggaggagc tggcggccat
     1801 cctggagatc agccgccgcc gcggcgtctg gctgatctcg gatgaggtgt attcccggct
     1861 ggtctatggc gcgctgacag ccgcgccctc gctgctcgac gtcgcggcgc cggatgaccg
     1921 ggtgatcgtc tgcaacagct tctccaagac ctgggtgatg accgggtggc ggctcggctg
     1981 gctggtcctg ccggacggca ctcgcgacgc ggtcagcgac atcgtggagg tgatccactc
     2041 cggcgtggcc cccttcatcc agcaggctgg tgtcgccgcg atcgacgatg ccgaggcgct
     2101 gcagcgcttc cgcgcctatt gcggtgccgg ccgtgccctg gcgagccagg cgctgtccgg
     2161 catcaacggc attcgctaca cgccgccggc cggcgcgttc tatgctttcg tgggcgtcga
     2221 aggactgacc gacagcctgg acctggcgct gaagctggtc acgaagcacc gtgtggcggt
     2281 cgctcccggc atcgccttcg gcgcggccgg cgaaggcagc ctgcggatct gtttcgccca
     2341 gtcacccgaa cggatggagc gtgccatgca gcgtttgcgc gacggattgc gggcggagct
     2401 ggccgcatga gcggcaccac gtcgccgcgc tgcgcccttt gcggcagcag ggcgcgcacg
     2461 ccgttccggg cgcccacccc cgagttggcg ccggacctgg acatgcgtcc gggcgagccg
     2521 gcctgggaaa cggtgcagga ctggctgcag acctgcgatc gctgcggcgc ggtcgccccg
     2581 gatctggcgg cactgccgga aacggccaag gccgtggtgc agtccgcggc ctaccgggac
     2641 ttggccgacg agaaatccga ggaaaccctg cccttccgcc gctgggcctt gctgtgccgc
     2701 gccgccggag attcgcaggc tgcgaccgag gcgatgctga tggccgcctg ggccgcggac
     2761 gacgcggtca gcatgctgga agcctcccgt ctgaggctga ccgttgcgga agcgtggggt
     2821 gagacggaag accaggcaac ggcattgtgg cggttggacg tgctgcgccg gtccagccac
     2881 ttcgccgcgg cggatgcctg ggcgaacacc ctggcggctc ggccactgga ccgggcgggc
     2941 aaagccatcc tggccttcca gcgggatcgc atcgcgcaac gcgacatcgg gcgccacctg
     3001 gtcaccagcg cattgccgcc cggggtcgag cacgagacgc tgaagcgccc cggcttctgg
     3061 tcctggttgt tccgcgagta ggtcagggga attcatacca gcggcgtgtc atgcccgggc
     3121 ttggcccggg cacccacgtc ttactgtgat gcatgcggca aatacgtggg tagccgggcc
     3181 aagcccggcc atgacacgga tgggatagaa cttcgggccg ctgatatcag tccccgcggg
     3241 ccgcgcgggc cagccggatc agttcgcggc gcacgccgcc cgggtcggac accggcgccg
     3301 accagggcac ccggacaaca cgttcgccgg ctgccaggtc gcagccgtca acgtcggcgg
     3361 ccaccatgcg ccacgcctcc cctgccccgc cgatcgccgc catggcgtcg gcatggtctg
     3421 cgttgcagtg cgcgatgatc tccggctccg ccgccgccac cgctgagaca gcagccgcat
     3481 cgggcgccag gtcggcggcg cgcagccgcg acgcacgggc gaacccgccg acgaacaggc
     3541 cgccgagcgg ccggatgcgc cagatcgcga aatcgccgaa atcggcgtag agcgcggaat
     3601 agggatgcag cgccacatag cgcgccttca gcgccgggtc gtccaggcgc tctgctaccc
     3661 cggtgacggt gacccggggc gcggtctgcg gattcgccgc ttccgctgca ccccgcacca
     3721 gcacggagca tcgcggttcg gcgcggagat gccgcgtgtg ctccgagagg tccgacagca
     3781 acagcaacac ggccagatcg ggtgccgtgg cgggcgtcac caggctagcg aaaggctgcc
     3841 cctgcgcgga ggtcgccaag gtgcccaccc gtgcggcccg cagcagcttg cgggcgaccc
     3901 acccgggcgc ttcgtcgctc atcgccaatc cctccttcgc tgccagatct gcgcggcact
     3961 gcgcatcatc cgctgaaaca ctgcaagcac ccccggcccg cggccgtgcc agcgaccgtg
     4021 accggccgcc cagggttgaa attcgcggcg gcgcccccac cggcgatttg ccaccgcccc
     4081 ccctatctgg catatcgccg cgccaacctc ccatcgactg ccggcgccga atcgccccgg
     4141 ccgcagcatg gcgctggccc aaacaaggat agagctgaag aaatgtccct ggcctacgct
     4201 gtgatcatcg cctgcggtgt gctggcgctg atctatggcc tgatcaccag tcgtcaggtc
     4261 ctcgccgccg acgccggcac cgcccggatg caggagatct ctggtgccgt gcaggaaggc
     4321 gcccgcgcct acctgaaccg gcaatatacc accatcgcca tcgtcggcgt ggtgatcatg
     4381 atcatcctgg gcgtcatcct cggcctgcac gtcgccatcg ggttcctgat cggcgcggtg
     4441 ctgtccggct ccgccggcta tatcggcatg aacgtgtcgg tgcgcgccaa tgtccgcacc
     4501 gcacaggcct ccaagcaagg tctcgccgcg ggcctcgaca tcgccttcaa atccggcgcc
     4561 atcaccggcc tgcttgtggt tggcctcggc ctgctcggca tttccatcta ttacctgatc
     4621 ctgcgctcca ccatgaccgg cacggacatt cggccggtgc tggaagccat ggtcgcgctg
     4681 ggcttcggcg gctcactgat ctccatcttc gcccggctcg gcggcggcat cttcaccaag
     4741 ggcgccgacg tgggtgccga cctggtgggc aaggtcgagg ccggcatccc cgaggacgac
     4801 ccccgcaacc ccgccgtgat cgccgacaac gtgggcgaca atgtcggcga ctgcgccggc
     4861 atggccgccg acctgttcga gacctacgcg gtgaccatcg tcgccaccat gctgctgtcg
     4921 gcgatctact tcaccggcag cgtgcgcgac tggctgctgc tgctgcccct gggcatcggc
     4981 ggcatctgca tcctgacctc ggtgatcggc acctacttcg tcaagctcgg cgccgacaac
     5041 gggatcatga aagcgctgta caagggcctg ctggtgaccg gtgcgctgtc cgtcatcggc
     5101 atcgccttca tcatcgcctg gttcgtcggc ttcggcgcgc cgctggcggt gaccaccggc
     5161 ggcagcatca ccggcatgtc gatcttcggc tgtgcgctgg tcggcttggc ggtgaccgga
     5221 ctgatcgtgt ggatcaccga atactacacc gcgaccgagt atcgtccggt gcgctcgatc
     5281 tcccaggcct ccaccaccgg tcacggcacc aacgtgatcc agggcctggc gatctccatg
     5341 gaggcgaccg cgctgccggt gctggtgatc tgcgtcggca tcatcgtcgc ttcgatcatc
     5401 gccggcctgt tcggcattgc catcgcggcg accaccatgc tgtcgctggc cggcatgatc
     5461 gtggcactgg acgcctatgg tccggtgacc gacaacgccg gcggcatcgc cgaaatggcg
     5521 ggattgccga aggaggtgcg caagtccacc gacgcgctcg acgcg
//
LOCUS       DASSZH010000610         3299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_406696, whole genome shotgun sequence.
ACCESSION   DASSZH010000610 DASSZH010000000
VERSION     DASSZH010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406696"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VFN42_13205"
     CDS             <1..306
                     /locus_tag="VFN42_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997620.1"
                     /translation="IARECYVGASNATAMEECRAALEYKYSSYAAWGMESPTAGMTFE
                     QMAKDRFIIGDKVAVKEEIARWQESLGVNHFIMRVQWPGLAQEKVLASIRRLGELFG"
     gene            347..622
                     /locus_tag="VFN42_13210"
     CDS             347..622
                     /locus_tag="VFN42_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997621.1"
                     /translation="MSDRQVPQLDMLPDGTFRQPARPPILTRVFFWASVVAVIAGGLA
                     IAAFLLWIALILVPVALLAGLIAWLAFRFQLWRRPGLAARQRDLWRS"
     gene            complement(1192..2232)
                     /locus_tag="VFN42_13215"
     CDS             complement(1192..2232)
                     /locus_tag="VFN42_13215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8997622.1"
                     /translation="MLRLSVLDQSTVVTGRSPDTSIRESLALAKHCEALGYHRYWCAE
                     HHNSESQAGTAPEILISAIAATTTRIRVGSAGVMLPHYSSLKVAEQFRVLEAIAPGRI
                     DLGLGRAPGSDGRTAYALNPLAESAADHFPAQVRDLLGWLAGEPLVEGHPFRDIRAQP
                     GGPTLPEVWMLGSSDYGAQVAAYFGLPFCFAHFITDGRGAEQALALYREGYRPSVRHP
                     EPHAALCVWAMAAPTQEEAARLFTSREMWRLGRDRGVYTALPSPEEAAAHSYTEGEQL
                     RIERLRARALYGTPDVVADKLRGVAESLGVEEVAVLTTLHDPEARRQSYTLLAAACGQ
                     AKQEDESTDAHR"
     gene            2416..>3299
                     /locus_tag="VFN42_13220"
     CDS             2416..>3299
                     /locus_tag="VFN42_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997623.1"
                     /translation="MAGATSTWTGVAGGDFAAAGNWDGEIVPSALDTALFAGSAGTVT
                     GDGTVLHIAIGDAASPYPGTWTFLGGSLAAGGEAIAGGLPDAVGFFGDTLLDGEILNA
                     AGGTTAIAGPAGVTVTARAGAQVTSGGDIVGGSGGQVGSLVLTGSGTDWIEQSGPVVD
                     GNLSGFLILGSLAGSSGTLAVDDGAQLLTGGGAVLGAAAGSCGSAAVAAGSWTASGGL
                     QVGVGGAGSLRVDAGTLTSGAATMLGVAAGSGGTLVIAGSGPGDPGLLTNAGDLVVGA
                     AGTGTLAAGENGVVTAGG"
BASE COUNT          484 a         1061 c         1181 g          573 t
ORIGIN      
        1 atcgcgcgcg aatgctatgt cggcgccagc aacgccaccg ccatggagga atgtcgcgcc
       61 gcactggaat acaagtacag ctcgtatgcg gcctggggca tggaaagccc gaccgccggg
      121 atgacgttcg aacaaatggc caaggaccgc ttcatcatcg gcgacaaggt cgcggtgaag
      181 gaggagatcg cgcgctggca ggaaagcctc ggcgtcaacc acttcatcat gcgcgtgcag
      241 tggcccgggc tggcgcagga gaaggtactc gcatcgatcc gccgcctggg cgaactgttc
      301 ggctgacgcc ttgaccgggc catggcgcgg cgccacttca tgcggcatga gcgatcgcca
      361 ggtaccccaa ctcgacatgc tgccggacgg aaccttccgc cagccggcgc gtccgcccat
      421 cctgacccgt gtgttcttct gggcgtcggt ggtggcggtg atcgccggcg ggctggcgat
      481 cgcggcgttc ctgctgtgga tcgcgctgat cctggtgccg gttgcgctgc tggccgggct
      541 gatcgcctgg ctggcgttcc gcttccagct ctggcgccgg ccgggcctgg ctgcccgcca
      601 gcgcgacctg tggcggtcct aggttccggg ttcaattagg aaccacagac gaacacggat
      661 gagtctggcg gttcgcagct ccgtccgctc cgtcagggca aattaatctt tggctatgct
      721 tgcgttggct gttggccggt tattactccc tggccccgac cgctgggggg cgctgcggca
      781 ccggaagaaa gaaagtcata cacagagttt cactaagtaa acgcagagtt tcacaaagaa
      841 aaattattga atgtttctca gtattatggc gccgtgttat agtaactatt tctaatcatt
      901 tttgtttaaa ctcgatatat acttagtaga attttgtgta tgccttgcgt tccttccgcc
      961 cggcggcatt gcgcggtgtg gttggcggcc atccctaccg atgcaggttc acggaccgag
     1021 gtgggttttg gctccaatct acccggtgcg agccccggga ggcggcacat acggtgccct
     1081 caattcgaaa ctggcgcctt tagcgcccag cggttaacgc aaccacaacc tgaatcttgc
     1141 cttcggcggg gcgcagccgc ggtggatcgg gccccaccgc accatccgtg ttcatctgtg
     1201 tgcatctgtg gattcatcct cctgcttggc ttgcccgcac gctgccgcca gcagcgtgta
     1261 ggactgccgc cgcgcctccg ggtcgtgcag cgtggtcagc accgcgactt cctccacccc
     1321 cagcgattcg gccacgccgc gcagcttgtc cgcgaccacg tccggcgtgc cgtacagcgc
     1381 ccgcgcgcgc agccgctcga tgcgcagctg ctcgccctcg gtgtagctgt gggcggcggc
     1441 ttcctcgggt gagggcaggg cggtgtagac gccgcggtcg cgccccagcc gccacatctc
     1501 ccgcgacgtg aacagccgcg ccgcttcctc ctgcgtcggc gcggccatcg cccagacgca
     1561 gagcgccgca tgcggctcgg gatggcgcac gctcggccgg tagccttcgc gatagagcgc
     1621 cagcgcctgc tccgcgccgc gcccgtcggt gatgaaatgc gcgaagcaga agggcaggcc
     1681 gaaatacgcg gccacctgcg cgccgtagtc ggagctgccg agcatccaga cctccggcag
     1741 ggtggggccg ccgggctggg cgcggatgtc gcggaacgga tggccttcca ccagcggctc
     1801 gccggccagc cagcccagca ggtcgcgcac ctgggccggg aagtgatcgg cggcggattc
     1861 cgccagcggg ttcagcgcat aggcggtgcg gccgtcggac ccgggcgcgc ggcccagtcc
     1921 caggtcgatc cgcccgggcg cgatcgcctc cagcacgcgg aactgctcgg cgactttcag
     1981 gctggaataa tgtggcagca tcaccccggc ggagccgacg cggatgcgcg tggtggtggc
     2041 cgcgatggcg ctgatcagga tttccggggc ggtgccggcc tgactttccg agttgtggtg
     2101 ctccgcgcac cagtagcggt ggtagccgag tgcctcgcaa tgtttcgcca gggcaaggct
     2161 ctcgcggatg gaggtgtccg gcgagcggcc ggtgaccacg gtcgattggt cgagcacgga
     2221 taggcgcagc atgaattcgc tcctgcgggt gctgcagatg aaagcgcggc ggggcggtcg
     2281 cgcaagagtg ggcccgcgtc gagccttcat ggcttgttaa cgtttcgctg gcaaaagcgc
     2341 ggcttgacgg ttggcgaagc ggccgaacca ggcggggcat tccgcgatcg ggatcgcttg
     2401 cagggggctg aacggatggc gggcgcgacc tctacgtgga ccggcgtcgc gggcggggac
     2461 tttgccgccg ctggcaactg ggacggcgaa atcgtgccgt ccgctctgga taccgccttg
     2521 tttgccggca gcgcgggcac cgttaccggg gacggcaccg tgctgcacat cgccataggc
     2581 gatgcggctt cgccttatcc cggcacctgg acgtttctcg gcggctccct ggcggccggt
     2641 ggggaagcca ttgccggcgg cctgccggac gcggtggggt tcttcggtga cacgctgctg
     2701 gacggggaaa tcctgaatgc cgccggcggc accaccgcga tcgccggtcc cgctggggtg
     2761 acggtcaccg ccagggcggg cgcgcaggtg accagcggcg gagacatcgt cggcggttcc
     2821 ggcgggcagg tgggctccct ggtgctgacc ggaagcggca ccgactggat cgagcagtcg
     2881 gggcccgtgg tggatggcaa cctgtccggc ttcctcatcc tcggcagcct cgccgggtcc
     2941 tcggggacgc tggcggtgga tgatggcgcc caactgctta ccggcggcgg cgccgtgctg
     3001 ggcgccgcag cgggaagctg cggcagcgcg gcggttgcgg cggggagctg gacggcgagc
     3061 ggcggcctgc aggtcggcgt gggcggcgcc ggcagcctgc gcgtcgatgc cggcacgctg
     3121 acctcgggtg cagccaccat gctcggcgtg gctgccggca gtggcggcac gcttgtcatc
     3181 gctggctccg gtcccgggga ccctggcctg ctgaccaatg ccggagacct cgtggtcggg
     3241 gctgccggta ccggcacatt ggcggcgggc gagaacggcg tggtcactgc cggcggcga
//
LOCUS       DASSZH010000611         3157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_628722, whole genome shotgun sequence.
ACCESSION   DASSZH010000611 DASSZH010000000
VERSION     DASSZH010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628722"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(8..69)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(162..2024)
                     /locus_tag="VFN42_13225"
     CDS             complement(162..2024)
                     /locus_tag="VFN42_13225"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8997624.1"
                     /translation="MERPKLTIPLARDATARFGMLLVAAVLVPLFTFLAVAWVDRVEV
                     QRNARQQAEDVADIAREQALGVFQTQELVAANVDQHIRGMNWDEITNSASLHAYLQRL
                     DVAYPQINGVWIADASGVLRSSSLRFPLPQVSIADRDYFRALRARDDGMFIGQMVRNA
                     VVSVLNFNVARRRETADGRFDGVVIVSALQSYLANFWRRISPQRDSITAIVRGDGMVL
                     ARQPELRPEAGGPPGVAQALPTNSVVHRLVTSGQARGSFRGISPIDGVERFYAVRRIG
                     DYNVYVVHGIGVSATLARWHADLRMFGGFFAVGGAALTFLAALALQQARRERIATDRW
                     RAAAQLANDEMQQRAAVEERLRHAQKMESLGQMAGSIAHDFNNILFVIGSSLEFLQGQ
                     QNDPRLERKVNVASDAVDRGARAIRSLLAFARRQPLHPKACNPATVLHGIQALLLQGL
                     GRQSRLVLDLAADTWDVMIDVSQFEMAVLNLTVNARDAMPKGGTLRIAASNERLGGDP
                     DGLAGEFVRLAVSDTGTGMAPEIAARAFEPFFTSKPVGKGTGLGLSQVYGFARQSGGT
                     ATLESAVGAGTTITLFLPRARPDSVVEGSAARVPTGAESPGVLSKPQVTEEADR"
     gene            2345..3004
                     /locus_tag="VFN42_13230"
     CDS             2345..3004
                     /locus_tag="VFN42_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HET8997625.1"
                     /translation="MMDDTTGKPREKLIARKQDWAREGRLLTGTTADPDLVRLPPGQR
                     EVSDWPVLDLGIQPDVTAEKFRLDIDGAVENPIHLTLAEFMALPQAESVSDMHCVTQW
                     SRYDNQWQGVAARTLLERARPKPTARHVIFHAHDGYTTNVRLDQFDAPDVFLVHTWNQ
                     QPIPRQHGGPVRVLIPRLYLWKSAKWVRRIELAAIDRPGFWETRGYHNNADPWLEERY
                     G"
     gene            complement(3008..>3157)
                     /locus_tag="VFN42_13235"
     CDS             complement(3008..>3157)
                     /locus_tag="VFN42_13235"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET8997626.1"
                     /translation="HALGLVVVADGVENEAQRAFLAGSGCDEGQGYLFSQPLPPELLA
                     ALPRS"
BASE COUNT          535 a         1054 c         1063 g          505 t
ORIGIN      
        1 cacgggttgc gcagcaggag tcatcagccc tcgcgggcgg ttttggggga ccccatcccc
       61 atgcgcgtgt gatgaaggga taaggcggag caaagcgggg cgcaagatgt gggcatatcg
      121 cgtgggccgc aacggccgcc gcgaccaggc atccggccgc ttcacctgtc ggcttcctcc
      181 gtcacctgcg gtttcgacag gacgccggga ctctcggcac ccgtgggaac acgcgccgcg
      241 gagccttcca ccacactgtc cggccgtgcc cgcggcagaa agagggtgat cgtcgtgcct
      301 gcgcccaccg cgctctccag ggttgccgtg ccgccggact gcctggcgaa gccatagacc
      361 tggctgaggc caagcccggt ccctttgccg accggcttgg aggtgaagaa aggctcgaag
      421 gcccgtgccg cgatctcggg cgccatgccg gtgccggtgt cggaaacggc cagtctcacg
      481 aactcgcctg cgaggccgtc cggatcgccg ccaagccgtt cattggacgc ggcaatccgc
      541 agggtgccac ccttgggcat cgcgtcgcgg gcgttcaccg tcaggttcag caccgccatt
      601 tcaaactggc tcacgtcgat catgacgtcc caggtatcgg cggcgaggtc cagtaccagc
      661 cggctttggc ggccaaggcc ctgcagcagc agggcctgga tgccgtgaag gaccgtggcg
      721 gggttgcagg ccttcggatg cagcggctgc cgccgggcga atgcgagcag ggagcggatg
      781 gcgcgcgcgc cccggtccac cgcatccgac gcgacgttga ccttgcgctc aagccgcggg
      841 tcgttctgct gaccctgcag gaattcaagg ctgctgccga tgacgaacag aatgttgttg
      901 aagtcgtgcg cgatgcttcc ggccatctgg ccgagcgatt ccattttctg agcatgccgc
      961 aaccgttcct cgacggccgc gcgttgctgc atctcgtcgt tggccagttg cgccgccgcg
     1021 cgccagcgat ccgtggcgat gcgctcccgc cgcgcctgct gcagcgccag cgcggcgagg
     1081 aaggtcagcg ccgcgccgcc gaccgcgaag aaaccgccga acatccgcag atcggcatgc
     1141 catcgcgcca gcgtggccga aacgccgatg ccgtggacga catagacgtt gtagtcgccg
     1201 atccgccgca ccgcgtagaa gcgttccacg ccatcgatgg gcgaaatgcc gcggaatgat
     1261 ccccgcgcct gtcccgacgt gacgaggcga tgcaccaccg aattggtcgg cagtgcctgc
     1321 gcgacgcccg gcggccctcc ggcttccggc cgcagttccg gctgacgcgc aaggaccatc
     1381 ccgtcgccgc gaacgatcgc ggtgatgctg tcccgctgcg gcgagatccg gcgccagaaa
     1441 ttcgcgagat agctttgcag ggccgatacg atgaccacgc cgtcgaagcg tccgtcggcc
     1501 gtctcgcgtc gccgggcgac gttgaaattc agcacggaga cgacggcgtt gcggaccatc
     1561 tggccgatga acatcccgtc gtcgcgggcg cgcagggcgc ggaaatagtc gcgatcagcg
     1621 atgctgacct gcggcagcgg aaatcggagg cttgaacttc gcagcacacc cgatgcgtcg
     1681 gcgatccaga cgccgttgat ctgcggataa gcaacgtcaa gtcgctgcag ataggcgtgc
     1741 agtgaagcgg agttggtgat ttcgtcccaa ttcatcccgc ggatatgctg atccacgttg
     1801 gccgcgacca gttcctgtgt ctggaacacg cccagggcct gttcgcgcgc gatatccgcg
     1861 acgtcctccg cctgctgcct ggcgtttcgc tgcacctcga cgcggtccac ccaggcgacg
     1921 gcgaggaacg tgaacaaggg caccagcacg gcggccacaa gcagcatccc gaaccgcgcc
     1981 gtcgcgtcgc gagccaaggg aatcgtaagt ttcgggcgtt ccatctcgga ttcccaactg
     2041 acttgcggcg ttttcagaac aagttattat gacgcatttt tgaactaact aatatagttc
     2101 acctttcgcg catgacaacc gtgacctgta attcattccc ggagtgcttt cgttgatctg
     2161 acgcaagccg gaaagtgttt gcccgaacgg ctgccgaatc ctccgccggc cgtccgtggg
     2221 ggcgcggggc atcatcggcg gttccccggc cgttcgcgcc ggtcggtcgt gccccgcctc
     2281 gccaaaccgg cgggcggcct gtagcgtccg ggcacttcga ggcattggcg gcggagcgag
     2341 gcagatgatg gacgacacga ccggcaagcc gcgggagaag ctgatcgcgc gcaagcagga
     2401 ctgggcgcgg gaggggcggc tgctcaccgg caccaccgcg gacccggatc tggtgcgcct
     2461 gccgccgggg cagcgcgagg tgtccgactg gccggtgctc gacctcggca tccagcccga
     2521 cgtgacggcg gagaagttca ggctcgatat cgacggcgcg gtggagaacc cgatccacct
     2581 gacgctcgcc gaattcatgg cgctgccgca ggcggagagc gtgtccgaca tgcactgcgt
     2641 gacgcagtgg agccgctacg acaaccaatg gcagggggtg gcggcgcgca cgctgctgga
     2701 gcgcgcgcgg ccgaagccga cggcccggca cgtcatcttc cacgcccatg acggctacac
     2761 gaccaatgtg cggctggatc agttcgacgc gcccgacgtg ttcctggtgc atacctggaa
     2821 ccagcagccc attccgcgcc agcatggcgg cccggtgcgg gtgctgatcc cgcgcctcta
     2881 tctgtggaag tcggcgaaat gggtgcggcg gatcgagctt gccgcgatcg accggccggg
     2941 gttctgggaa acccgcggct atcacaacaa cgccgatccg tggctggagg aacgctacgg
     3001 ctgagcctca gcttcgcggc agggcggcca gaagctccgg cggcagcggc tggctgaaca
     3061 gatagccctg gccctcgtcg cagccgctgc cggcgaggaa ggcgcgctgc gcctcgttct
     3121 ccaccccatc cgccaccacg acgaggccga gcgcgtg
//
LOCUS       DASSZH010000612         2601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1463817, whole genome shotgun sequence.
ACCESSION   DASSZH010000612 DASSZH010000000
VERSION     DASSZH010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463817"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..1598)
                     /locus_tag="VFN42_13240"
     CDS             complement(36..1598)
                     /locus_tag="VFN42_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006559285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HET8997627.1"
                     /translation="MPARPEWRPRYNPWLVALVVTLAAFMEVLDTTIVNVSLPHIAGS
                     MGASTDEATWTLTSYLVANGIVLPISAWLGNLLGRRRYFLICIAAFTLFSFLCGIATN
                     LGELILFRLLQGFFGGGLQPNQQAILLDTFPAAQRGRAMSVVAIATVIAPVLGPTLGG
                     VITDSASWRWVFLLNVPVGILCMIGVAAVVEDPPWAQRIARRIDAIGLGLIALGIGSL
                     QVMLDRGEDADWFASGFIRVAAVLAVIGMVGATLWLLWTKHPVVNIRTLKDRNFAVGC
                     LMISATMAVLYSSAVTLPVLAQTVFGYTSTLAGYILSPGALALLVMIPIVGKLMGMVQ
                     TRFLVFIGFMLLGAGLVYSRRLTPDIDFDTLMTIRIAQSIGLGFLFVPISTIAYTTLP
                     PQQRADAVALNVMFRNIAGSIGIALATAMVTSGAQVHMAHLSAHLTPLDPAYVDLLHH
                     DVQTLVAMGHSTQTATGMAMGRIYRSLTTQATIMAYMDLFRWAALAAFISAPFALLFT
                     GAKSSMRPGGGH"
     gene            complement(1601..>2601)
                     /locus_tag="VFN42_13245"
     CDS             complement(1601..>2601)
                     /locus_tag="VFN42_13245"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HET8997628.1"
                     /translation="GVLVLIIGGGWYWYSARNEVATDDAYTDGDVVTVAPQVSGQVVE
                     RDVTDNQHVTPGQVLVRIDPRPFLAARDQAKGQLDAAEGQLAAAQAALDLAKQVYPAR
                     LAAAQAERDAADAVLFRAQTDLKRQKALPRIATTQQAIDQAVAAQRQAAAELEQANAA
                     VRQAEPVQQNIAQAAAQVKQLQGVVAQAHAQLQQAEINLGWTTVQAAQEGWVTKRNVD
                     TGNYVGAGSAILSLVTPHVWISANFKETQLDRMRPGDAVDISVDAYPGLRLHGHVDSV
                     QLGSGSRFSAFPAENATGNFVKIVQRVPVKIDIDSGLDPNLPMPLGISVEPTVHVK"
BASE COUNT          448 a          901 c          856 g          396 t
ORIGIN      
        1 acatcgccgc ggattacgcg attgtagcga tggtgtcagt gaccgccgcc cggccgcatc
       61 gacgacttgg cgccggtgaa cagcagcgcg aacggcgcgg agatgaatgc cgccagtgcc
      121 gcccagcgga acaggtccat ataggccatg atcgtcgcct gcgtggtcag cgagcggtag
      181 atgcggccca tcgccatgcc cgtcgccgtc tgtgtcgagt ggcccatcgc caccagcgtc
      241 tgcacgtcgt gatgcagcag gtccacatag gccggatcga gcggcgtcag atgcgccgaa
      301 agatgcgcca tgtgtacctg cgcgcccgat gtcaccatcg cggttgccag cgcgatgccg
      361 atggacccgg cgatattgcg gaacatgacg ttcagcgcca ccgcatcggc gcgctgctgc
      421 ggcggcaagg tcgtataggc gatcgtgctg atcggcacga acaggaagcc gaggccgatg
      481 ctctgcgcga tgcggatggt catcagcgtg tcgaagtcga tgtcgggcgt cagccgccgt
      541 gaatagacca gccctgcgcc gagcagcatg aagccaatga agaccaggaa gcgcgtctgc
      601 accatcccca tcagcttgcc gacgatcggg atcatcacca gcagcgccag ggcacccggc
      661 gaaaggatgt agcccgccag cgtcgaggta tagccgaaca ccgtctgcgc cagcaccggc
      721 agcgtcaccg ccgaggaata cagcaccgcc atggtggcgg agatcatcag gcagccgacc
      781 gcgaagttgc ggtccttcag cgtgcgaatg ttgaccaccg gatgcttcgt ccagagcagc
      841 cacagcgtcg cgccgaccat tccgatgacg gccagcaccg ccgcgacacg gatgaagccg
      901 gaggcgaacc agtcggcatc ctccccgcgg tcgagcatca cctgcaggct gccgatgccg
      961 agcgcgatca ggccaagccc gatggcatcg atccgccgcg cgatgcgctg cgcccagggc
     1021 gggtcctcga ccaccgcggc gacgccgatc atgcacagga tgccgaccgg cacgttgagc
     1081 aggaacaccc agcgccacga ggcactgtcg gtgatcacgc cgccgagcgt cgggccgagc
     1141 accggcgcga tcacggtggc gatggcgacc accgacatgg cccggccgcg ctgggcggcg
     1201 gggaacgtat ccagcaggat cgcctgctga ttcggctgca gcccgccgcc gaagaatccc
     1261 tgcagcagcc ggaacaggat caactcgccc aggttggtcg cgataccgca caggaagctg
     1321 aacagcgtga acgcggcgat gcagatcagg aaataccgcc tgcggccgag caggttgccg
     1381 agccaggcgg aaatcggcag cacgatgccg ttggccacca gataggacgt gagcgtccag
     1441 gtcgcctcgt cggtggaggc gcccatcgag ccggcgatgt gcggcagcga cacgttgacg
     1501 atggtggtgt cgagaacctc catgaacgcg gcgagcgtca ccaccagcgc caccagccac
     1561 ggattgtaac gcggccgcca ttccggccgc gccggcatgc tcatttcaca tgcaccgtgg
     1621 gctcgaccga aatgcccagc ggcatcggca ggttcgggtc caggccactg tcgatgtcga
     1681 tcttcaccgg cacgcgctgc acgatcttca cgaagttgcc ggtcgcgttc tcggccggga
     1741 aggcgctgaa cctgctgccg ctgccaagct gcacgctgtc cacgtggccg tgcagcctca
     1801 gccccggata ggcatcgacg gagatgtcca cggcatcgcc cggccgcatg cggtcgagct
     1861 gggtttcctt gaaattggcg gagatccaga catgcggcgt caccagcgac aggatggcgc
     1921 tgccggcacc gacatagttg cccgtgtcca cgttgcgctt cgtcacccag ccttcctgcg
     1981 ccgcctgcac ggtggtccag ccgagattga tctcggcctg ctgcagttgc gcgtgcgcct
     2041 gcgcgaccac gccctgcaac tgcttcacct gcgccgcggc ctgcgcgatg ttctgctgca
     2101 ccggctccgc ctggcgcacc gcggcattgg cctgttccag ctccgccgcc gcctggcgct
     2161 gtgcggcgac cgcctggtcg atcgcctgct gcgtcgtcgc gatgcgcggc agggctttct
     2221 ggcgcttgag gtcggtctgc gcccggaaca gcaccgcatc cgcggcatcg cgctccgcct
     2281 gcgcggcggc gaggcgcgcc ggatagacct gcttcgccag atccagtgcc gcctgcgccg
     2341 cggcgagctg gccttcggct gcgtccagtt gccctttcgc ctggtcgcgc gcggcgagga
     2401 acgggcgcgg gtcgatcctg accagcacct ggccgggtgt cacgtgctgg ttgtcggtca
     2461 cgtcgcgctc gaccacctgc ccgctcacct gcggcgccac cgtcaccacg tcgccatcgg
     2521 tataggcgtc gtcggtggcg acctcgttgc gggcggaata ccagtaccag ccaccaccga
     2581 tgatcagcac cagcacgccc a
//
LOCUS       DASSZH010000613         3262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_117071, whole genome shotgun sequence.
ACCESSION   DASSZH010000613 DASSZH010000000
VERSION     DASSZH010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117071"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="VFN42_13250"
     CDS             complement(<1..971)
                     /locus_tag="VFN42_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET8997629.1"
                     /translation="MSLTDLTIAEARDGLRARRFSAEELTLAHLYAIEALNPRLNAYI
                     TVSHAQAVDQARAADAALARGEGRALTGIPLAIKDLFCTAGVRTTAGSKILGPFVPPY
                     ESTVTANLLRDGATFLGKTNMDEFAMGSSNMSSAYGPVENPWKRRQDAEAVLVPGGSS
                     GGSAAAVSARLALGGTGTDTGGSIRQPASFCGIVGVKPTYGRCSRWGVVAFASSLDHP
                     GPFARTVRDSAILLGSMAGHDPQDSTSADLPVPDFEAACARGVKGLRVGVPREYRSDG
                     MPAEIDALWQQGVAWLRDAGAEIIDVSLPHTKYALATYYIVAPAEASS"
     gene            complement(968..1255)
                     /gene="gatC"
                     /locus_tag="VFN42_13255"
     CDS             complement(968..1255)
                     /gene="gatC"
                     /locus_tag="VFN42_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET8997630.1"
                     /translation="MSLDPATVRRIATLARIRVEDSEIPTLQAELNGILGWIEQLNAV
                     NVDGVEPLTGAARMALKMRDDAVTDGGYPDKVLANAPDRADSYFAVPKVVE"
     gene            complement(1345..1692)
                     /locus_tag="VFN42_13260"
     CDS             complement(1345..1692)
                     /locus_tag="VFN42_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997631.1"
                     /translation="MTIARGAATALLGCLLLGACTFGSPPLTGQERANQETVSACRDR
                     AEQVYDLQHRPDIYRPPPEINTPASGAFSPVGMTDRRLSDLYARDQMVSDCVRNTGTE
                     TSRGQQPTLRNAR"
     gene            complement(1689..2231)
                     /locus_tag="VFN42_13265"
     CDS             complement(1689..2231)
                     /locus_tag="VFN42_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997632.1"
                     /translation="MQTREHPPYVRVGDGPDGSVTYLVDMPPESLPTVCGRDLERAWY
                     AARNAALAQSWGSVRGFRFRRSDGSHTDLALADRDARCWAGAVDGTVGIGTSYGLSIC
                     LRLLALIDLLAHARWALPLCHLARDGAELHPSLLRAAAAHPLTNEARFDETGFRLRLG
                     EIAGMRLEAATAARLTGASA"
     gene            2316..>3262
                     /locus_tag="VFN42_13270"
     CDS             2316..>3262
                     /locus_tag="VFN42_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET8997633.1"
                     /translation="MTNDPAGLTATEAVRRIRDGRLSAMELMEACLARIAARDPVVRA
                     FAWFDAAAARDAARAAPAGPLHGLPIGVKDVLDTADMASGYGSPIWAGWRPRADAAAV
                     AWARAAGAVVMGKTVTTEFATRKPGATTNPANPDHTPGGSSSGSAAGVAAGFFPLAYG
                     TQTAGSVIRPAAYCGVVGYKLSFGTINRFGMKLMSDSLDTVGVMARSVADCALFAGAV
                     SGRDLGDPAMKPADAPRIGICRSPSWHLAGAETQGLLDRVASALARAGAALSERELPA
                     EYNALEQAHPIVMNSESARALGWELAHHRDGISPVLLERL"
BASE COUNT          495 a         1099 c         1212 g          456 t
ORIGIN      
        1 gaggacgcct cggccggcgc gacgatgtag taggtggcga gggcgtattt cgtgtgcggc
       61 agggagacgt cgatgatctc ggcgccggcg tcgcgcagcc aggccacgcc ctgctgccac
      121 agcgcatcga tctccgccgg catgccgtcg gagcggtact cccgcggcac gccgacccgc
      181 agtcccttca cgccccgcgc acaggccgcc tcaaagtccg gcaccggcag gtcggcgctg
      241 gtggaatcct gcggatcatg cccggccatc gagcccagca ggatggcgct gtcgcgcacg
      301 gtgcgggcga agggcccggg atggtccagc gaggaggcaa acgccaccac gccccaacgc
      361 gagcagcggc cataggtcgg cttcacaccg acaatgccgc agaacgaggc cggctggcgg
      421 atcgatccgc cggtatccgt cccggtgccg cccagtgcca gccgcgcgga aaccgcagcg
      481 gcggacccgc cggaagagcc gccgggcacc agcacggctt cggcatcctg ccgccgcttc
      541 cacgggttct ccaccgggcc gtaggccgag ctcatgttgg acgagcccat ggcgaactcg
      601 tccatgttgg tcttgccgag gaaggtcgcg ccgtcgcgca gcaggtttgc ggtgacggtg
      661 ctctcgtagg gcggcacgaa ggggccgagg atcttgctgc cagcggtggt gcgcacgccg
      721 gcggtgcaga acaggtcctt gatcgccagc ggaatgccgg tcagcgccct gccctcaccc
      781 cgtgccagcg ccgcatcggc ggcacgcgcc tggtccacgg cctgggcgtg gctgacggtg
      841 atgtaggcgt tcagccgcgg gttcagcgcc tcgatcgcgt acagatgcgc cagggtcagc
      901 tcttctgcgc tgaaccgcct ggcgcgcagc ccgtcgcgtg cttcggcgat ggtcagatcg
      961 gtgaggctca ttccaccacc ttcggcaccg cgaaatagct gtcggcgcgg tccggtgcat
     1021 tggccagcac cttgtcgggg taacccccgt cggtcaccgc gtcatcgcgc atcttcaacg
     1081 ccatgcgggc ggcaccggtc agcggctcca cgccgtccac attcaccgcg ttgagctgct
     1141 cgatccagcc caggatgccg ttcagctccg cctgcagcgt cgggatttcg ctgtcctcga
     1201 cacggatgcg ggcgagcgtg gcaatgcgac ggaccgtcgc gggatcgagc gacatgcaga
     1261 ttctcttgtc tgggagaccg aaccccgact ttaccggccg tccgggcagg gttcaaccgc
     1321 gcccgctgcg ctgcgctcgc gccatcaacg cgcgttgcgc agggttggct gctggccgcg
     1381 gctggtctcg gtgccggtgt tgcgcacgca gtcgctgacc atctggtcgc gggcatacag
     1441 gtcggacagg cgccggtcgg tcatgccgac cggggagaac gcgccagaag ccggcgtgtt
     1501 gatttccggc ggcgggcggt aaatgtcggg gcggtgctgg aggtcataca cctgctccgc
     1561 ccgatcgcgg caggccgaga cggtttcctg attcgctcgt tcctggccgg tcagcggcgg
     1621 cgacccgaag gtgcaggcac ccagcagcag gcagcccagc agcgccgtgg cagcgccccg
     1681 tgcgatggtc atgcggaagc tccggtcagg cgggcggcgg ttgctgcttc cagccgcatc
     1741 ccggcgatct cgccgagtcg gaggcggaag ccggtctcgt cgaaccgcgc ctcattggtg
     1801 agaggatggg cggcggcggc gcgcagcagg gacggatgca gttccgcccc gtcgcgcgcg
     1861 aggtggcaga gcggcaacgc ccagcgcgca tgcgccagca ggtcgatcag cgccagcagg
     1921 cgcaggcaga tggacaggcc atagctggtg ccgatcccga ccgtgccatc cacggcgccg
     1981 gcccagcagc gcgcatcgcg gtcggccaag gccaggtcgg tgtgggagcc gtcggagcgg
     2041 cggaaacgga agcctcgcac cgaaccccag gactgcgcca gcgcggcgtt gcgcgcggca
     2101 taccaggcgc gctccaggtc gcggccacac accgtcggca gggattccgg cggcatgtcc
     2161 acaaggtagg tgacggatcc gtccgggccg tcgccgactc ggacataggg ggggtgctca
     2221 cgcgtctgca tcaatggcaa gatggacccg ggctgcgtga cgcgccaggg ggatcggcgt
     2281 ggggatcggc gcggcggcga gggaaagagg acaggatgac gaacgatccg gccggactga
     2341 ccgccaccga ggcggtgcgc cgcatacgcg acgggcggct gagcgccatg gagctgatgg
     2401 aagcctgctt ggcgcggatt gcggcgcggg acccggtggt gcgggccttc gcctggttcg
     2461 atgcggcggc cgcgcgagac gcggcgcgcg cggcgccggc cggaccgctg cacgggttgc
     2521 ccatcggggt aaaggacgtg ctggacaccg ccgacatggc cagtggctac ggctcgccga
     2581 tctgggctgg ctggcgcccc cgggccgatg ccgccgcagt ggcctgggca cgggcggcgg
     2641 gcgcggtggt gatgggcaag acagtcacta cagagttcgc cacgcgcaag ccgggcgcca
     2701 cgaccaaccc ggccaatccg gaccacaccc cgggcggctc gagcagcggg tcggcggcag
     2761 gcgtcgcggc ggggttcttc ccgctcgcct atggcaccca gaccgcgggc agcgtgatcc
     2821 ggccggcagc ctattgcggg gtggtgggct acaagctgag cttcggcacc atcaaccggt
     2881 tcggcatgaa gctgatgtcc gacagcctgg atacggtcgg ggtaatggcg cgctcggtgg
     2941 ctgactgcgc gctgttcgcc ggggcagtgt cgggacgaga cttgggcgat ccggcgatga
     3001 agccggcgga cgcaccgcgc atcggcatct gccggtcgcc gagttggcac ctggcgggag
     3061 cggagacgca gggactgctg gaccgcgtgg cctcggccct ggcacgggcc ggcgcggcgc
     3121 taagcgagcg cgaactgccg gccgaataca acgccctgga acaggcacac ccgatcgtca
     3181 tgaacagcga aagcgcccgc gcgctgggct gggaactggc gcatcaccgc gacgggatca
     3241 gcccggtcct gctggagcgg ct
//
LOCUS       DASSZH010000614         2092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1668156, whole genome shotgun sequence.
ACCESSION   DASSZH010000614 DASSZH010000000
VERSION     DASSZH010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1668156"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VFN42_13275"
     CDS             <1..492
                     /locus_tag="VFN42_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8997634.1"
                     /translation="IEAGSRRSAFASVDLAPPLGDLAELYEAVAEEKGVTLTAEIPER
                     LPAYGDRELIQQAVANLLDNAVKFSPAGGTVRLRAGAVPNGVEVAVIDEGPGIPQEDL
                     KRATERFFRGESARSTPGAGLGLALVQAVAQLHGGTLRLEDARPGLAARLTVPAGEQP
                     ANA"
     gene            553..861
                     /locus_tag="VFN42_13280"
     CDS             553..861
                     /locus_tag="VFN42_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997635.1"
                     /translation="MTASRTHGTAGLLLSLAVGLAITHGSMAGQTAPVLVTTDTPQYC
                     LHLLDQVSELMRLAPSAPPEVSSLSTEGQRMCGQGQTRGGIMRLRRALVLLKQQNLPP
                     "
     gene            complement(1105..2001)
                     /locus_tag="VFN42_13285"
     CDS             complement(1105..2001)
                     /locus_tag="VFN42_13285"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET8997636.1"
                     /translation="MAELHGIIPPTTTPFTADGEIDFAAARAQLRWLIGQGVHGVATG
                     GSTGEGHTLEVDEFRRLVADTVEETAGRVPVVAGIIVDSTRDAIRRGKAVAELGVAAL
                     QVTPVHYLFRPSDDAMLDHFRALTEATGLPVIIYNVVPWSYLSPALLIRIMREVPGVI
                     GVKQSAGDLKLVADLMTMVPPGKRIFSAVDALLYPSFALGAHGSIAAILTAAPRACVD
                     LWDAVQAGDHRRALDLHTRLLALWNAMVGDNLPACTKFAQTLQGCPGGLPRAPMPPAS
                     EAQQAAIRAALRGVLGQEKRAA"
BASE COUNT          312 a          722 c          753 g          305 t
ORIGIN      
        1 atcgaagcag gctcccgccg ctccgccttc gcctcggtcg atctggcgcc ccccttgggc
       61 gatctggcgg agctgtatga ggcggtcgcg gaggagaagg gcgtcacgct gaccgcggaa
      121 atccccgaac gcctgccggc ctatggcgac cgtgagctga tccagcaggc ggtcgccaac
      181 ctgctggaca acgcggtgaa attctcgccg gccggcggca cggtgcggct gcgcgccggc
      241 gccgtgccga acggcgtgga agtcgcggtg atcgatgagg ggcccggcat tccgcaggag
      301 gacctgaagc gggccaccga gcgcttcttc cgcggcgaat ccgcccgcag cacgccgggc
      361 gcggggctcg gcctggcgct ggtgcaggcg gtggcgcagc tgcatggcgg aacgctgcgg
      421 ctggaggatg cgcgcccggg cctggccgcc cgcctgaccg tgccggcggg cgagcaaccg
      481 gccaatgcct aacacgcttg tcacctgcga aacatcgggg cgagagcccg cctatgtgac
      541 aagtgtgcca tgatgaccgc gtcgcgcacc catggcaccg cgggcctgtt gctgtccctg
      601 gccgtcggcc tggcgattac gcatggctcg atggcgggcc agaccgcccc tgtcctggta
      661 accaccgata cgccgcaata ctgcctgcat ctgctggatc aggtcagcga actgatgcgg
      721 ctggcaccgt cggctccgcc cgaggtgagc agcctttcca ccgagggtca gcgcatgtgc
      781 ggccagggcc agacccgcgg cggcatcatg cgcctgcgcc gggcgctggt gctgctgaag
      841 cagcagaacc tgccgccgtg agcgagagga attcatcatt cgggaatcgg cgactcgctt
      901 gcaatccggt gtggtgcggt ccagatttcc cgccaggcag caacaactga aaggaggtga
      961 tccagtgtct cattgttcgg tggcgtccct tgccgtgacc tggatcgccg gcgcgaacgc
     1021 gacggcgtaa cattctccgg tccggcgacg gacagtcata gacaatgcga aggccccggg
     1081 ggcgaccccg gggccttcgg catttcaggc cgcccgtttc tcctgcccga gcacgccgcg
     1141 cagcgccgcg cggatcgcgg cctgctgcgc ctcggaggcc ggcggcatcg gcgcgcgcgg
     1201 cagcccgccc gggcagcctt gcagcgtctg cgcgaacttg gtgcaggccg gcaggttgtc
     1261 acccaccatc gcattccaca gcgccagcag ccgcgtgtgc aggtccagcg cgcggcggtg
     1321 gtcgcccgcc tgcaccgcgt cccacagatc gacgcaggcg cgcggcgccg cggtgaggat
     1381 cgccgcgatc gagccgtgcg cgcccagcgc gaaggacggg tagagcagcg catcgacggc
     1441 ggagaagatg cgcttgcccg gcggcaccat ggtcatcagg tcggccacca gcttcaggtc
     1501 gccggcgctc tgcttcaccc cgatcacgcc gggcacctcg cgcatgatgc ggatcagcaa
     1561 cgcaggcgac agataggacc agggcaccac gttgtagatg atcaccggca gcccggtggc
     1621 ctcggtcagc gcgcggaagt ggtccagcat ggcgtcgtcg ctggggcgga acaggtagtg
     1681 caccggggtg acctgcagcg ccgcgacgcc gagttccgcc accgccttgc cgcggcggat
     1741 cgcatcgcgg gtggaatcga cgatgatgcc cgccaccacc ggcacgcggc cggcggtttc
     1801 ctccaccgtg tcggcgacca gccggcggaa ttcgtccacc tccagcgtgt ggccctcgcc
     1861 ggtggagccg ccggtggcga ccccgtgcac gccctggccg atcagccagc gcaactgggc
     1921 gcgggcggca gcgaagtcga tctcgccgtc agcggtgaac ggcgtggtgg tgggtgggat
     1981 gatgccgtgc agctctgcca tggcggtcct ccgttgtgtg gtgcggggag tctcgctccg
     2041 gcgcgcccgc ggtcaagccg gcaaccgccg cgctggcagg gatcagtagc gg
//
LOCUS       DASSZH010000615         2282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_798853, whole genome shotgun sequence.
ACCESSION   DASSZH010000615 DASSZH010000000
VERSION     DASSZH010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798853"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..787
                     /locus_tag="VFN42_13290"
                     /pseudo
     CDS             1..787
                     /locus_tag="VFN42_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227165.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
     gene            802..1539
                     /gene="fabG"
                     /locus_tag="VFN42_13295"
     CDS             802..1539
                     /gene="fabG"
                     /locus_tag="VFN42_13295"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019090256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HET8997637.1"
                     /translation="MFRLDGKAALITGASGGIGATIARTLHAQGAAVVLSGTRRDALD
                     ALAAELGDRAFVCQADLSDPAAAEALGTAAEEAAGPLHVLVNNAGFTRDMLALRMSDE
                     DWGAVLDVDLSAPFRLARAALRGMLRRRAGRVINISSIVGATGNAGQANYAAAKAGLV
                     GLTKALAQEVASRGITVNVVAPGFIATPMTDKLSAQQKEKLSGAIPLGRLGQPNDVAA
                     AVAYLASDEAAWVTGATLHVNGGMAML"
     gene            1804..2043
                     /locus_tag="VFN42_13300"
     CDS             1804..2043
                     /locus_tag="VFN42_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011253554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET8997638.1"
                     /translation="MSEIADRVKKIVVEHLGVEESKVTPEASFIDDLGADSLDTVELV
                     MAFEEAFSVEIPEDAAEKISTVKDAIDYIEKQKAA"
     gene            2073..>2282
                     /locus_tag="VFN42_13305"
     CDS             2073..>2282
                     /locus_tag="VFN42_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HET8997639.1"
                     /translation="MRRVVVTGMGIACPLGIGVEHVWKRLINSESGITAIQSFDTKDL
                     PCKVAGTVPQGVAADGKLDISEWVPV"
BASE COUNT          358 a          760 c          830 g          334 t
ORIGIN      
        1 tacccagcca gccctgatgg cacactccat cgcggtgctg cgggtgctcg agcgggaagc
       61 cggcatcagc ctgaagcaga aggccgcggt cgtggccggc cactcgctgg gcgagtacag
      121 cgccctggct gccgccggcg ccttctccct gtccaacgcc gcccggctgc tgaagctgcg
      181 tggccaggcg atgcagaagg cggtgccacc ggggcagggc gccatggcgg cgctgcttgg
      241 tgccgatatg tcacaggcgc aggcgatctg cgagcaagcc gcgccggtgc cggacagcga
      301 aacgcaccag cgttaggtgg ttcagccggc caacgataac ggggccggcc aggtggtcat
      361 ctccggccat cgcgaggcgg tggaacgcgc catcccgatc gccaccgcca tgggaatccg
      421 ccgcgccatg ctgctgccgg tctccgcccc gttccactgc gcgctgatgg cgccggcggc
      481 ggacgcgatg gccgaggcgt tcgagacgac gccccccggc gcgccgctgg tgccgctgat
      541 cgccaatgtc tccgccgcca aggcgaccga cccagcggag atccgcgacc tgctggtccg
      601 ccaggtcacc gccacggtgc gctggcgtga gtgcgtgcag gcgatggtcg cgctcggcgt
      661 ggacagtttc gtggaactcg gcgccgggaa agtgctgtcc ggactggtgc ggcgcatcgc
      721 gccggatgca aaagccgcct cggccggcac tccggccgat atcgaggcgt tgctgaagaa
      781 tctctgacgg aggaacgaac catgttccgg ttggacggca aggcggcgct gattaccggc
      841 gcttcgggcg gcatcggcgc gacgatcgcg cggacgttgc atgcgcaggg cgcggcggtg
      901 gtgctgtccg gcacgcggcg ggacgcgctg gacgcgctgg cggcggaact gggcgaccgc
      961 gccttcgtct gccaggcgga tctgagcgac ccggctgctg ccgaggcgct ggggaccgcg
     1021 gccgaggaag cggccggtcc gctgcacgtg ctggtgaaca acgccggctt cacccgcgac
     1081 atgctggcac tccgcatgag cgacgaggat tggggcgcgg tactcgacgt ggatctgtcg
     1141 gcgccgttcc gcctggcgcg ggccgcgctg cgcggcatgc tgcgccggcg ggcagggcgg
     1201 gtcatcaaca tctcctccat cgtcggcgcc accggcaatg ccggccaggc caactatgcc
     1261 gccgcgaagg ccggcctcgt gggtctgacc aaggcgctgg cgcaggaggt tgcctcccgc
     1321 ggcatcacgg tgaacgtcgt ggcgccgggc ttcatcgcca cgccgatgac cgacaagctg
     1381 tccgcccagc agaaggagaa gctgtcgggc gccatcccgc tgggccggct gggccagccg
     1441 aacgacgtgg cggcggcggt ggcctacctg gcctccgacg aggcggcctg ggttaccggc
     1501 gccacgctgc acgtcaacgg cgggatggcg atgctgtgag agtgtccccc gtgtcatggc
     1561 caggcttggc ccggtcaccc acgtctttgc ggcggcccac ccagcaagtc gtggatggcc
     1621 gggccgggcc cggccatgac acggaagcgt ccccctcccg gccttcctcg gcctgggaaa
     1681 ccatgctaag cggcccgcgt cttcgtccgg cgcgggtgcg gcggcaccga tcgggtcctg
     1741 cccggcggcc aatcaaaccg ggcgaatgtt gatgcgggac cccaatccag gagtgcggag
     1801 ccgatgagcg agattgccga tagagtgaag aagatcgtgg tggaacacct cggcgtcgag
     1861 gaatccaagg tgacgcccga ggcgtccttc atcgacgacc tgggcgccga cagcctggat
     1921 acggtcgagc tggtcatggc cttcgaggaa gccttctcgg tggaaatccc cgaggacgcc
     1981 gccgagaaga tcagcaccgt gaaggacgcg atcgactaca tcgaaaagca gaaggccgcc
     2041 tgaatcggcg tccgagggag gattcgcagc tgatgcgacg tgtcgtggtg acgggcatgg
     2101 gcattgcctg tcccctgggg atcggggtcg agcatgtctg gaagcgcctg atcaattccg
     2161 agtcggggat caccgccatc cagtccttcg ataccaagga cctgccctgc aaggtcgccg
     2221 gcacggtgcc gcaaggcgtg gccgccgatg gcaagctgga catctccgaa tgggtcccgg
     2281 tc
//
LOCUS       DASSZH010000616         3112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_782032, whole genome shotgun sequence.
ACCESSION   DASSZH010000616 DASSZH010000000
VERSION     DASSZH010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782032"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..1168)
                     /locus_tag="VFN42_13310"
     CDS             complement(155..1168)
                     /locus_tag="VFN42_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997640.1"
                     /translation="MQPQHAPATLPGATVDAYNAEIRDESGFVGDHATNRAFQSILCQ
                     WRERLRAAGEDPLGDRATEDISRRRLEQVLLHGDPEAAGLVLSAVEEFAQELVRVCRR
                     LLALDAWADTQRIAVGGGFRASRLGELAIGRAGVLLRAAVGVEMRPIHHDPDQAGLLG
                     AAMLAPGGIAAEQAVLAVDIGGSKIRVGRVGRSDQGDALRVLDTAIWAHAEERPDRGE
                     AVGRLAGMLRAMADKAGDDPARSLAPFVGIGCPGLIRNDGTIARGGQNLPGDWEAPGF
                     NLPQQLTQHLPPIQGRTPLVLLHNDAVAQGLSEAAWMQDVRRWGIFTIGTGLGNARFT
                     NRG"
     gene            1378..1578
                     /locus_tag="VFN42_13315"
     CDS             1378..1578
                     /locus_tag="VFN42_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997641.1"
                     /translation="MKFRSYGPMDLPTALMWAVLGLGGYAAWLTSIVFCSTSSSWPLM
                     IAGMLFFPVGVVHGVGLWFGGW"
     gene            1664..1924
                     /locus_tag="VFN42_13320"
     CDS             1664..1924
                     /locus_tag="VFN42_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997642.1"
                     /translation="MDDTFIDRMGRRFRAVCKTGGMRAGTQKPVFHAVTPDCRIALCG
                     AEPGPRSAWGEPAGAVTCPACLTRLSRLQGAPRQAGQPNRVS"
     gene            complement(1917..>3112)
                     /locus_tag="VFN42_13325"
     CDS             complement(1917..>3112)
                     /locus_tag="VFN42_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997643.1"
                     /translation="FGASDTIDLYLINATSLSFANGVLTVMNGTTAVESLPMSGSYTT
                     ANFQMMLQPGSEFSNAITYVPDASGSGSGSGSTSPQVNAPASETVVAGGTLAIPGVSV
                     TDSFAAANPGSMALNVTDTSGTLRMTDAGGAALAGSGTAAIHYSGSLAEVNAALATLS
                     YAAGSAASDSITVDIWDQAGKEATGSIGVTVGGSAATPTPTPVPTPTPTPTGGSVISG
                     TPGDDTIVSNLNNVTINAGAGNNTIFAGGTGDTITAADGNNTVQAFAGGNTISTGAGN
                     DTIRIAGSANTVNAGGGINQISDSGSGNTLVLPGAGQGTDNVYGYVLAGNDTFDLRSA
                     LASAGWQGSQATLPDFLAVGTAHGGADTTISVTPGGGAGSVVATLYGNGAIPLASFLQ
                     HALVS"
BASE COUNT          456 a         1059 c         1077 g          520 t
ORIGIN      
        1 agcggtcgcg gagctgagac ggccggcgac ggagatcatc aggatgcccg atttgacgcc
       61 gcagatgggc ctgcaggtct acgtggatgc tctgggcgtc ctcattctgt ttctgcacat
      121 catcatcaag atccacgcca agggaaagaa atgatcatcc gcgattggtg aagcgcgcat
      181 tgcccaggcc ggtgccgatg gtgaagattc cccagcgccg gacgtcctgc atccaggctg
      241 cctcgctcag cccctgcgcc acggcgtcat tgtgcagcag caccagcggc gtgcggccct
      301 ggatcggcgg cagatgctga gtcagctgtt gcggcaggtt gaagccaggc gcttcccaat
      361 cgcccggcag gttctgcccg ccgcgggcga tcgtcccgtc attccggatc aggcccgggc
      421 agccgatgcc gacaaacggc gccaggctgc gcgcagggtc atcgccggcc ttgtcggcca
      481 tggcgcgcag catcccggcc agccggccga ccgcctcgcc gcggtccggc cgctcctcgg
      541 catgcgccca gatcgccgtg tccagaaccc ggagggcatc gccctggtcc gaacgcccca
      601 cccgcccgac gcggatcttg gaaccgccga tatccaccgc cagtaccgcc tgctccgccg
      661 cgatcccgcc gggcgcgagc atcgccgcgc cgagcagccc ggcctgatcc ggatcgtgat
      721 ggatcggccg catctccacg ccgaccgcag cgcgcagcaa cacgccggcc cggccgatcg
      781 ccagctcgcc cagccggctg gcgcgaaatc cgccgccgac ggcgatgcgc tgggtgtcgg
      841 cccatgcatc cagcgcgagc agccggcggc acacccggac cagttcctgg gcaaattcct
      901 ccaccgcgct gagcaccagc ccggccgctt ccggatcgcc gtgcagcagc acctgttcca
      961 gccggcgacg gctgatgtcc tcggtcgcgc ggtcgcccag cgggtcttcc ccggcggcgc
     1021 gcagccgttc ccgccattgg caaaggatcg actggaacgc acggttcgtg gcgtggtcgc
     1081 cgacgaaccc gctctcgtca cggatttccg cgttgtaggc gtcgaccgtc gcgccgggca
     1141 gagttgccgg cgcatgctgc ggctgcacga tgttggacct tttgcagaac gacattccgg
     1201 tggcagcaac gccgtgaacg cgccgagggt tcagtagggc gtgacctggg cttgcgccgc
     1261 agggtccctg tcggattctt aatgaaacgg cgcggcacga tggcgcaaaa tgcccagcag
     1321 cagtgccgaa ccgtgcgacg gcaagctgca acctggcctc ggtgcaatgg gggagcaatg
     1381 aagttccgca gttacggccc gatggatctg ccgactgccc tgatgtgggc cgtgctcggc
     1441 ttgggtggct acgccgcgtg gctcacgtcg atcgtcttct gctccaccag cagttcctgg
     1501 ccgctgatga tcgccggcat gctgttcttc ccggtcggcg tggtgcacgg ggtcggcctg
     1561 tggttcggcg gctggtgacg gcggcgcaat aagtccaggc ggaatattcc gcactgcaat
     1621 atcgccttga cggcaacaaa gcgcagcggc atgcagccgc tccatggacg acacgttcat
     1681 tgacagaatg ggccgccggt tcagggcggt atgcaagacc gggggaatgc gtgccggcac
     1741 gcagaagccg gtgttccatg cggtgacccc ggattgccgg atcgccttgt gtggcgccga
     1801 accgggcccg cgctccgcct ggggcgaacc ggccggggcg gtcacctgcc ctgcttgcct
     1861 cacccggctg tcccgcctgc agggcgcgcc caggcaggcg ggacagccga accgggtcag
     1921 ctgaccagcg cgtgctgcag gaagctggcc agcgggattg cgccgttgcc gtatagcgtc
     1981 gccacgaccg acccggcgcc gccacccggc gtgaccgaaa tcgtggtgtc tgctccgcca
     2041 tgcgccgtgc cgaccgccag gaagtccggc agcgtggctt gcgagccttg ccagccggca
     2101 ctggcgagag cggagcgcag gtcgaacgtg tcgttgccgg ccagcacgta gccgtacacg
     2161 ttgtcggtgc cctggccggc gcccggcagc accagggtgt tgccgctgcc gctgtccgag
     2221 atctggttga tgccgccgcc ggcattcacc gtattcgccg aaccggcgat ccggatcgtg
     2281 tcattgcccg ccccggtgct gatggtattg ccgccggcaa aggcctggac agtgttgttg
     2341 ccgtccgccg cggtgatggt gtcgccggtg ccgccggcga agatggtgtt gttgccggcc
     2401 ccggcattga tcgtgacgtt gttcaggttg gagacgatcg tgtcgtcgcc cggcgtcccg
     2461 ctgatcacgc tgccgccggt cggcgttggc gttggagtgg ggaccggtgt gggcgtcggc
     2521 gtggcggcac tgccgcccac ggtcacgccg atcgaccctg tggcctcctt gccggcctgg
     2581 tcccagatat cgacggtgat gctgtcggat gccgcggagc cggcggcata ggacagcgtg
     2641 gccaaggcgg cgttcacctc ggccaggctg cccgagtaat ggattgcggc ggtgcccgac
     2701 ccggccagtg cggcgccgcc ggcatcggtc atccgcagcg tgccgctggt gtcggtgacg
     2761 ttcagcgcca tgctgcccgg attggccgcg gcgaagctgt cggtgacgct gacgcccggt
     2821 atggccagcg tgccgccggc caccacggtt tcgctggccg gcgcgttcac ctgcgggctg
     2881 gtgctgccgg aacccgaacc cgaccctgaa gcatccggca cgtaggtgat cgcgttggag
     2941 aattccgagc cgggctggag catcatctgg aagttcgctg tggtgtagct gccggacatc
     3001 ggcagcgact ccacggccgt ggtgccgttc atcaccgtca gcacgccatt ggcgaaggac
     3061 aggctggtgg cgttgatcag gtacaggtcg atggtgtcgc tcgcgccgaa ac
//
LOCUS       DASSZH010000617         2891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1225632, whole genome shotgun sequence.
ACCESSION   DASSZH010000617 DASSZH010000000
VERSION     DASSZH010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225632"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="VFN42_13330"
     CDS             complement(<1..914)
                     /locus_tag="VFN42_13330"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HET8997644.1"
                     /translation="MTETILSRASGGGTVKPGDVVVVDVETAVLMDMTFLPEGWREIL
                     KVHDPRKVAIVFDHLVPAPTIQAATAMRTGRQFAAKFGIGRLHDVGYDQGIAHAVVAD
                     RAYAVPGTVLVNGDSHTCAAGAFNCAARGVGGPDMLFAVTTGKAWFRLGETVRYDLTG
                     RLPDGVSAKDIFLTIAGEYGAHVNQNVEYGGPGMSTLSINGRRTLATMGTELSAEFVI
                     FEPDDALLDYVRARNQQPFEPARPDPDAVYADRRSIDLGSLEPLVALPDAVVNNSVKI
                     SQVAGQKIDQAFIGSCANGTLDDLEVAAR"
     gene            1077..2294
                     /locus_tag="VFN42_13335"
     CDS             1077..2294
                     /locus_tag="VFN42_13335"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET8997645.1"
                     /translation="MNDSAASVVRPIIDSPAFRAARATLEAEHERTVQDIITLTEIPA
                     PSFQEQQRAQAFLEMARAHGLQDLEIDAEGNVTGIRRGIGNGRLICVAAHLDTVFPPG
                     TPIKVRREGNKLFAPGIGDDTRSLAVLLAWLRALDAAAIRTRADILFVADVGEEGPGD
                     LRGMRHLFQRGPYKDRISAFITVDSPNMEHIATGGVGSKRYRVTFTGPGGHSYGAFGL
                     VNPMYAMADAITRLGRIPVPTRPKTTYSASVTGGGTSINSIPNSVWTEFDLRSESPHE
                     LDRLEQAFLLLVREAVTAENAARSIQRGAISADLTKIGDRPAGHTDEAGELPSLAKAA
                     LAAHGFKASFETSSTDANIPLSLGIPAIKIGSGGTGGRAHSLEEWIDVTPEHSVRGMA
                     AGLATLLAVAGMG"
     gene            2380..2643
                     /locus_tag="VFN42_13340"
     CDS             2380..2643
                     /locus_tag="VFN42_13340"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET8997646.1"
                     /translation="MELRELRERGVLHAGLFGSVARNEATPASDIDIVIELAPDAAID
                     LFDYVGLTQYLADLFPHRVDVANLRRLKPLVRPSVERDAVYAF"
BASE COUNT          461 a          972 c          984 g          474 t
ORIGIN      
        1 cgggcggcga cctccaggtc gtccagcgtg ccgttggcgc aggagccgat gaaggcctgg
       61 tcgatcttct gcccggccac ctggctgatc ttcaccgagt tgttcaccac cgcgtcgggc
      121 agcgccacca gcggttccag gctgcccagg tcgatgctgc ggcgatctgc atagaccgcg
      181 tccgggtcgg gccgagccgg ctcgaagggc tgttggttgc gcgcgcgcac gtagtccagc
      241 agcgcgtcat cgggttcgaa gatgacgaat tcggcggata gttcggttcc catcgtcgcc
      301 agcgtgcggc ggccgttgat gctgagcgta gacatgcccg ggccgccata ctcgacgttc
      361 tggttcacat gcgcgccata ctcgccggcg atggtcagga agatgtcctt ggccgagacg
      421 ccgtccggca gcctgccggt caggtcgtag cgcacggtct cgccgaggcg gaaccaggcc
      481 ttgccggtgg tgacggcgaa cagcatgtcg ggtccgccga cgccgcgcgc ggcgcagttg
      541 aacgcgcccg cggcgcaggt gtgggaatcg ccgttcacca gcacggtgcc gggcaccgca
      601 taggcgcggt ccgccacgac ggcgtgcgcg atgccctggt cgtagccgac atcgtgcagg
      661 cgcccgatgc cgaatttcgc ggcgaactgc cgcccggtgc gcatcgccgt ggcggcctgg
      721 atggtgggcg ccggcaccag gtggtcgaac acgatcgcca ccttgcgcgg atcgtgcacc
      781 ttcaggattt cgcgccagcc ctccggcagg aaggtcatgt ccatcagcac ggcggtttcc
      841 acgtccacca cgacgacgtc acccggcttc accgtgccgc cgccgctggc gcgggaaaga
      901 atcgtttccg tcatggtcat gcccattgga ccgtcccccc ttttgctgaa atcagcatgg
      961 gctggaacgg gcggccgggc aatccatggc atcggcttgt gggcatggca tcggcttgaa
     1021 gccgctgccc gaagctgcaa gtaacggccc tgccgcacac gagaggattg tcctgcatga
     1081 acgacagcgc cgcttccgtc gtccgcccga tcatcgacag ccccgcgttc cgcgccgccc
     1141 gcgccacgct ggaagcggag catgagcgca ccgtacagga catcatcacg ctgacggaaa
     1201 tccccgcgcc gtccttccag gaacagcagc gtgcccaggc cttcctggag atggcccgcg
     1261 cgcacgggct gcaggatctg gagatcgacg ccgagggcaa cgtcactggc atccgccgcg
     1321 gtatcggcaa tggccggctg atctgcgtcg ccgcgcacct ggacacggtg tttccgcccg
     1381 gcacgccgat caaggtcagg cgcgagggca acaagctgtt cgcccccggc atcggcgacg
     1441 acacccgcag cctggcggtg ctgctggcgt ggttgcgcgc gctggacgcg gcggcgatcc
     1501 gcacgcgcgc cgacatcctg ttcgtcgccg atgtggggga ggaagggccc ggcgacctgc
     1561 gcggcatgcg ccacctgttc cagcgcggac cgtacaagga ccggatcagc gccttcatca
     1621 cggtggacag cccgaacatg gagcacatcg ccacaggcgg cgttggctcc aagcgctatc
     1681 gcgtcacctt caccggtccg ggcggacaca gctacggcgc gttcgggctg gtcaacccga
     1741 tgtacgccat ggcggacgcc atcacccggc tcggccgcat cccggtgccg acccggccga
     1801 agaccaccta cagcgccagc gtcaccggcg gcggcacgtc gatcaactcc atccccaaca
     1861 gtgtgtggac ggagttcgac ctgcgctcgg aatcgccgca cgaactggac cggctggagc
     1921 aggctttcct gctgctggtg cgcgaagccg tcaccgcgga gaacgccgcg cgctccatcc
     1981 agcgcggcgc gatcagtgcg gacctgacca agatcggcga ccgcccggcc ggtcacaccg
     2041 atgaggcggg cgaattgccg tcgctggcga aggcggcgct ggcggcgcac gggttcaagg
     2101 ccagcttcga gacctcgtcc accgacgcca atatcccgct cagcctggga attccggcga
     2161 tcaagatcgg ctccggcggc accggcggcc gggcgcactc gctggaggag tggatcgacg
     2221 tcacgccgga gcacagcgtg cgcggcatgg cggccggact ggcgacgctg ctggcggtgg
     2281 ccgggatggg gtaggcgcgt accggcttct ggtgttctcc aggaacacgc tataaggcgc
     2341 ggatgaaccg cgaacaggtg attgccaggc tccgcctgca tggaactacg ggaactacgg
     2401 gagcgcggtg tgctgcatgc cggcttgttc ggctccgttg cgcgcaatga ggcgacgccg
     2461 gcgagcgaca ttgatatcgt gatcgagctt gcacccgacg ccgcgatcga cctgttcgac
     2521 tatgttggcc tgacgcagta cctcgctgat ctttttcccc atcgcgtgga tgtcgccaac
     2581 ttgcgccgcc tgaagccttt ggtgcggccg agcgtcgaac gggatgccgt gtatgccttt
     2641 tgaccgaacg cgcctggcgc tggaacttta gccgctgatg atccgggctc ggaattgcga
     2701 gacctgagct ttcagcttcg aatctccttc ggcaagacgc gggtgaccgg cctgcgccgg
     2761 ccatgacacg ggggggaggt gctgaggagc gcttcaggct gctcggcggg cgggcatcgc
     2821 acgcgcatcg tccaggatca tgtcggatgc cttctccgca atcatgatcg tcggggcatt
     2881 ggtgttcccc g
//
LOCUS       DASSZH010000618         3387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_747899, whole genome shotgun sequence.
ACCESSION   DASSZH010000618 DASSZH010000000
VERSION     DASSZH010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747899"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /gene="hisA"
                     /locus_tag="VFN42_13345"
     CDS             <1..759
                     /gene="hisA"
                     /locus_tag="VFN42_13345"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006115679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HET8997647.1"
                     /translation="PEEPGPGLMPLTLYPAIDLKDGQCVRLKRGEMDQATVYSADPAA
                     QARAWQDAGFTWLHVVDLNGAFAGHPVNEAAVQAILGAVTLPVQLGGGIRDLPRIAAW
                     LEAGVRRVILGSAAAKNPPLVLEACRSFPGRIVVGIDAHDGFVATEGWAERSTMPATD
                     LALRFEDAGVAAIVYTDIGRDGMLSGLNLEQTVALAQRVSTPVIASGGVGALADLAAL
                     RDAAAGTRIEGVIVGRALYDGRVRPDEALALLGG"
     gene            892..1662
                     /gene="hisF"
                     /locus_tag="VFN42_13350"
     CDS             892..1662
                     /gene="hisF"
                     /locus_tag="VFN42_13350"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HET8997648.1"
                     /translation="MLKLRVIPCLDVKDGRVVKGVNFVSLRDAGDPVEQAAVYDAAGA
                     DELTFLDITASHENRDTILDVVSRTAARVFLPLTVGGGIRSIEDMRRLLLAGTDKCSI
                     NSAAVARPELVREAAQKFGSQCVVVAVDAKQTGAERWEVFTHGGRRPTGIDAIQWCRQ
                     VVELGAGEILLTSMDRDGTGQGFDLALLRAACAAVRVPVIASGGVGTLQHFVEGAQAG
                     ATGLLAASVFHFGTFTIAQVKAALAAAGLPVRLPQLAA"
     gene            1670..2176
                     /locus_tag="VFN42_13355"
     CDS             1670..2176
                     /locus_tag="VFN42_13355"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003622337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HET8997649.1"
                     /translation="MAKSTKKHAAPKRDPLVTRAKKTRKPKKPKAVKTPAERERPAPP
                     LALGPNAAVLDRLYAVVLSRRETDPAVSHSARLLSRGAAKVAQKFGEEAVECLIEGVA
                     GNHDGVVAESADVLYHLIVLWASAGVTPDEVWAELKRREGISGIAEKASRAKQLPIAF
                     GLETTKIP"
     gene            2198..2575
                     /locus_tag="VFN42_13360"
     CDS             2198..2575
                     /locus_tag="VFN42_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007399936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HET8997650.1"
                     /translation="MPVSGQPPYDDNNIFARILRGEIPSKTVYEDQWALAFHDIAPQA
                     PQHVLVIPKGKYCSFADFSATAPAEEIAGFIRAVGTVAKQLGMEAEGYRLLANMGPNS
                     GQEVPHLHVHLFAGRPLGRMIAG"
     gene            complement(2723..>3387)
                     /locus_tag="VFN42_13365"
     CDS             complement(2723..>3387)
                     /locus_tag="VFN42_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET8997651.1"
                     /translation="GLAGGPVVLAGYCMGGTLAVAAAQLRPELVSALALLAAPWDFHA
                     ADPERALAAARCLPLLEPLLAFDHTLPIDGLQTLFALLDPFGVAEKYRSFARLDPDSA
                     RAVLFVALEDWLNDGIPLAAPVARETLSGWYGENTPARGAWRIAGLPVDPALLRLPVF
                     VAAPGRDRLVPPESARPLAALIPGATLHEPVAGHIGMVAGGKAEQALWRPLSAWLRRA
                     GR"
BASE COUNT          557 a         1181 c         1192 g          457 t
ORIGIN      
        1 ccggaagaac caggaccagg gctgatgccg ctgacgctgt atcccgcgat cgacctgaag
       61 gacggccagt gcgtccgcct gaagcgcggc gagatggacc aggccacggt gtactccgcc
      121 gatcccgccg cccaggcgcg cgcctggcag gacgccggct tcacctggct gcacgtggtg
      181 gacctgaacg gcgcctttgc cgggcatccg gtgaacgagg ccgcggtgca ggcgatcctc
      241 ggtgccgtca ccctgccggt gcagctcggt ggcggcatcc gcgacctgcc gcgcatcgcc
      301 gcctggctgg aagccggagt gcgccgggtc atcctgggca gcgccgcggc gaagaacccg
      361 ccgctggtgc tggaagcctg ccgcagcttc cccggccgca tcgtcgtggg aatcgacgca
      421 catgacgggt tcgtcgcaac cgaaggctgg gcggagcgct cgaccatgcc ggcaaccgac
      481 ctggcgctgc gcttcgagga tgccggcgtg gcggcgatcg tctacaccga tatcggccgg
      541 gacggcatgt tgtccggact gaacctggag cagacagtgg cgctggcgca acgggtgagc
      601 acgccggtga ttgcctccgg cggggttggc gcgctggcgg atttggcggc gttgcgcgac
      661 gccgcggcgg gaacccggat cgagggggtg atcgttggcc gcgcgctgta cgacggccgc
      721 gtgcggccgg acgaggcgct ggcgctgctg gggggataga ctctcccccc tcccgcaagg
      781 ggacggggcg tctcgtggtg cagcacggca cccaacaacc accggactca tccagcggca
      841 tcaaggcaag cctcttgcca cggtcacgcc gcatcggtct aagactcccc catgctgaaa
      901 ctccgtgtca tcccgtgcct ggacgtgaag gacggccgcg tcgtcaaagg cgtcaacttc
      961 gtctcgctgc gcgacgccgg cgatccggta gaacaggccg cggtgtacga cgcggcgggc
     1021 gccgatgaac tgaccttcct tgacatcacc gccagccacg agaaccgcga caccatcctg
     1081 gacgtggtct cccgcaccgc cgcgcgggtg ttcctgccgc tgaccgtcgg cggcggcatc
     1141 cgctcgatcg aggacatgcg ccgcctgctg ctggccggca ccgacaagtg cagcatcaac
     1201 tccgccgcgg tggcacggcc ggagctggtg cgcgaggcgg cacagaaatt cggcagccag
     1261 tgcgtcgtgg tggcggtgga cgccaagcag accggcgcag agcgctggga ggtgttcacc
     1321 catggcggcc gtcgccccac cggcatcgat gcgatccaat ggtgccggca ggtcgtggaa
     1381 ctcggcgccg gggaaatcct gctgaccagc atggaccgcg acggcaccgg ccagggcttc
     1441 gatctggcgc tgctgcgcgc cgcctgcgcc gcggtgcggg tgccggtgat cgcctccggc
     1501 ggggtcggca cgctgcagca tttcgtcgaa ggcgcgcagg ccggcgccac cgggctgctt
     1561 gccgccagcg tgttccattt cggcaccttc accatcgcgc aggtgaaggc agcgcttgcc
     1621 gccgccgggc tgccggtgcg gctgccacag ttggcggcct gaggcagcga tggcgaaatc
     1681 caccaagaag catgcggcgc ccaagcggga cccgctggtc acgcgggcaa aaaagacgcg
     1741 gaagccgaag aagccgaaag ccgtcaagac gccggccgaa cgggagcggc cggcaccgcc
     1801 gctggcattg ggaccgaatg cggcggtgct ggaccggttg tatgcggtgg tgttgagccg
     1861 gcgcgagact gaccctgcgg tgagccactc cgcccggctg ctgtcgcgcg gcgccgccaa
     1921 ggtggcgcag aaattcggcg aggaggcggt ggagtgcctg atcgaaggcg tcgccggcaa
     1981 ccatgacggc gtggtggccg agagcgcgga cgtgctctac cacctgatcg tgctatgggc
     2041 ttccgccggc gtgacgccgg acgaggtctg ggccgaactg aaacgccgcg aaggcatcag
     2101 cggcatcgcc gagaaggcgt cgcgcgccaa gcaactgccg attgccttcg ggctggagac
     2161 gacaaagatt ccctgaccct gagccaggag aagcgccatg cccgtgagcg gccagccgcc
     2221 ttatgacgac aacaacatct tcgcgcgcat cctgcgcggg gagatcccca gcaagacagt
     2281 ctatgaggac cagtgggcgc tggcgttcca cgacatcgcc ccgcaggcgc cgcagcatgt
     2341 gctggtgatc ccgaagggca agtactgctc cttcgccgat ttcagcgcca ccgcgccagc
     2401 ggaggaaatc gccggcttca tccgcgcggt cggcacggtg gcgaagcagc tcggcatgga
     2461 ggccgagggc taccggctgc tcgccaacat gggaccgaac agcggacagg aagtgccgca
     2521 tctgcacgtg cacctgtttg ccgggcggcc gctgggacgg atgatcgccg ggtaatcgcg
     2581 cccgcaacca gcagcatgca ccgtcgcggc gggatcgtcg cggcggggtt cgtcgtcgtg
     2641 gcgggaaggc ccgccaccca cgactttgcc gcatgccgtc aaggtaattc gtgaatggcc
     2701 ggccttccag ccgggacctt cttcatcgcc ccgcgcggcg cagccacgct gacaagggcc
     2761 gccacagcgc ctgctccgcc ttgccgcccg ccaccatgcc gatatggcct gccaccggtt
     2821 cgtgcagcgt cgcgccaggg atcagcgccg ccagcggacg cgccgattcc ggcggcacca
     2881 gccggtcgcg gccgggcgcg gcaacgaaca ccggcagccg caacagcgcg ggatccaccg
     2941 gaaggcccgc gatacgccac gctccgcgtg ccggcgtgtt ctcaccatac cagccggaca
     3001 gcgtctcgcg tgccaccggc gcggcaagcg gaatgccgtc gttcagccag tcctccagag
     3061 cgacgaacag cacggcccgc gcactgtccg gatccagccg agcgaagctg cggtatttct
     3121 ccgccacccc gaacgggtcg agcaacgcga acagcgtctg caacccgtcg atcggcagcg
     3181 tgtggtcgaa ggccagcagc ggctccagca gcggcaggca gcgtgccgcg gccagcgcgc
     3241 gttccggatc agccgcatgg aaatcccacg gtgcggccag cagcgccagc gccgacacca
     3301 gctcgggccg tagctgcgcc gcggccaccg ccagcgtgcc gcccatgcag tagcccgcca
     3361 gtaccaccgg gccaccggcc agaccgc
//
LOCUS       DASSZH010000619         4568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1634325, whole genome shotgun sequence.
ACCESSION   DASSZH010000619 DASSZH010000000
VERSION     DASSZH010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1634325"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..100
                     /locus_tag="VFN42_13370"
     tRNA            17..100
                     /locus_tag="VFN42_13370"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50..52,aa:Leu,seq:caa)
     gene            complement(105..1049)
                     /locus_tag="VFN42_13375"
     CDS             complement(105..1049)
                     /locus_tag="VFN42_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET8997652.1"
                     /translation="MEVRNLGQSGLRVSAIGLGCNNFGGRIDKDASRRVIHKALDLGI
                     TLFDTADTYGERGGSETEMGEILGDARKRIVLATKFGNPMDESGEKSGGSRRYIMQAV
                     EASLRRLRTDWIDLYQIHRPDPRTPIEETLRAMDDLVRQGKVRYIGCSNFPAWQMVEA
                     QWTARANGLTAFVSCQDEYSLLVRGVVEPQLRPAMLKYGMGLLPFFPLASGLLTGKYR
                     RNAPMPGGARLSRIQRLADRYLTDTNWATAEKLGDFAEAHGHTLLELAFSWLLAQAPV
                     ASVIAGATRPEQLEQNVTAGGWRLTEDDLAEIDRLTAA"
     gene            complement(1122..3011)
                     /locus_tag="VFN42_13380"
     CDS             complement(1122..3011)
                     /locus_tag="VFN42_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET8997653.1"
                     /translation="MARGRTDHAGTVSDANPRSSPDVPDRSERPPPIEDYALIGDCET
                     AALVSKAGSIDWLCWPRFDDGACFAALLGTPRNGRWQIAPQDAPGAVHRRYRPGTLIL
                     ETVFETPHGSVALLDFMVPNAHSVVRILECRSGHVAMAFDLALRFEYGSAIPWVTRLP
                     HRNGVRAVAGRHQVVVHSDVPLHGEDMTTKARFTVRSGQRVRFVMTHAPSHIEPPEPP
                     GPDAALQQTEAWWTAWTRRCTHQGEWREAVHSSLITLKALTYAPTGGIVAAPTTSLPE
                     QPGGARNWDYRFCWLRDATFTLLALMHAGYREEAQAWGKWLRRTVAGTPDQVQTLYRL
                     SGERWILESEVPWLAGYRGASPVRVGNAASTQVQLDVYGELMDALYQEVQSHLAKPSE
                     SWDLQTALIGHLEEIWDRPDESIWEVRGSPRHFTFSKAMVWVALDRAIRGAEQHGLKA
                     PLERWRALRDHVHAQVCDQGFNAAKNSFVQYYGADELDASLLLLPLVGFLPPHDPRIK
                     GTVAAIERELMVDGLVRRYNTRASIDGLQGDEGVFLACSFWFVDNLVLQDRRDEARAM
                     FERLLALRNDVGLLAEEYDPVANCQLGNFPQAFSHLALVNAALNLENSHGPAQKRRRR
                     DHPPG"
     gene            complement(3011..3373)
                     /locus_tag="VFN42_13385"
     CDS             complement(3011..3373)
                     /locus_tag="VFN42_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997654.1"
                     /translation="MIKNLPKHTLRTIGLAAMGAGLVVSAGLATAQARVFVGFGMPLY
                     FGPPVYAPPPPVYYPPPVYYAPPPVYAPPQGYAPYQAGTGQSCYAGAYVCPMDRPVSP
                     GSGCYCVVNGTQVWGHAS"
     gene            complement(3447..>4568)
                     /locus_tag="VFN42_13390"
     CDS             complement(3447..>4568)
                     /locus_tag="VFN42_13390"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HET8997655.1"
                     /translation="YEPWAREAYAALAAELAKQGVALHRMRTATLFQPEAVKTRSGTP
                     FGVFTPFSRRCLELGVPEAAYPRPERIPCPPPPASDRLEAWDLLPRPDWAGGLRAAWV
                     PGEAGAQDRLHRFIRDALPAYSEARDLPGAAGTSMLSPHLRFGEISPVQLWHALPEAE
                     GRAAFRREVLWREFAYHLLWHHPALPETPLLPDFARMPWRDDQPALRAWQRGRTGIPI
                     VDAGMRQLWQTGWMHNRVRMIAASFLVKHLLLPWQQGEAWFWDTLVDADLANNAAGWQ
                     WVAGSGADAAPYFRIFNPVLQGRKFDPQGDYVRRFVPELAGLSPSAIHAPWDVPESDL
                     ARAGIRLGHAYPRPLIDLAAGRRRALDALAVMKRTGTHA"
BASE COUNT          751 a         1629 c         1518 g          670 t
ORIGIN      
        1 tatgatgggc gcagaggcga gagtggcgga acggtagacg cagtcgactc aaaatcgacc
       61 gccgcaaggc gtgggggttc gaatcccccc tctcgcacca gccgctatgc ggccgtcaga
      121 cgatcgatct ccgccagatc gtcctcagtc agccgccagc ccccggccgt gacgttctgt
      181 tccagctgct cgggccgcgt cgcgccggca atcacgctgg ccaccggcgc ctgcgccagc
      241 agccaactga acgccaactc cagcagcgta tgcccgtgcg cctcggcgaa gtcgccgagc
      301 ttctcggcgg tggcccagtt ggtgtcggtc agatagcggt cggccaggcg ctggatgcgc
      361 gacagccgcg cgccgccggg catcggcgca ttgcgccggt atttcccggt cagcagcccg
      421 ctggccagcg ggaagaaggg cagcagcccc atgccgtatt tcagcatcgc cggccgcagc
      481 tgcggctcca ccacgccgcg caccagcagc gagtattcgt cctggcacga gacgaacgca
      541 gtcaggccgt tggcgcgcgc cgtccactgc gcctccacca tttgccaggc gggaaaattg
      601 gagcacccga tgtaccgcac cttcccctgc cgtaccaggt cgtccatcgc ccgcagcgtc
      661 tcctcgatcg gcgtgcgcgg gtcggggcgg tggatctggt acaggtcgat ccagtcggtg
      721 cgcaggcggc gcaggctggc ttccaccgcc tgcatgatgt agcggcgcga accgcccgac
      781 ttctcgccgg actcgtccat cgggttgccg aacttggtgg ccagcacgat ccgcttgcgc
      841 gcatcgccga ggatctcgcc catctcggtt tccgagccgc cgcgctcgcc ataagtatcg
      901 gcggtgtcga acagcgtgat gcccaggtcg agcgccttgt ggatcacccg ccgcgacgca
      961 tccttgtcga tccggccgcc gaaattgttg caacccagtc cgatcgccga cacgcgcagg
     1021 ccggactggc cgaggtttcg aacttccatg cgactcaccg aagctggttc atcaccagct
     1081 tcgccccacc cgccaggcgc gtcaaagcaa cgcgcgggat gtcacccggg cggatggtcg
     1141 cgcctgcggc gcttctgcgc cggaccgtgc gaattctcca ggttgagcgc cgcattcacc
     1201 aacgccaggt gggaaaatgc ctgcgggaaa ttgccgagct ggcagttggc gaccgggtcg
     1261 tattcctcgg cgagcaggcc gacatcgttg cgcaacgcca gcagccgttc gaacatcgcg
     1321 cgcgcctcat cgcggcggtc ctgcagcacc aggttgtcga cgaaccagaa gctgcaggcg
     1381 aggaacacgc cctcgtcgcc ttgcagcccg tcgatgctcg cgcgcgtgtt gtaacggcgc
     1441 accaggccat ccaccatcag ctcgcgctcg attgccgcga ccgtgccctt gatgcgcggg
     1501 tcgtgcggcg gcaggaagcc gaccagcggc agcagcagca ggctggcatc cagctcgtcc
     1561 gcaccgtagt actgcacgaa gctgttcttc gcggcgttga agccctgatc gcagacctgc
     1621 gcgtggacgt ggtcgcgcaa cgcccgccac cgctccagcg gcgccttcaa cccgtgctgc
     1681 tcggcgccgc gaatcgcccg atccagcgcc acccacacca tggccttgga gaaggtgaaa
     1741 tgccgcgggc tgccccgcac ctcccagatg ctttcatcgg gccggtccca gatctcctcc
     1801 agatgcccga tcagcgccgt ctgcagatcc cagctttcgc tcggcttcgc caggtgggat
     1861 tgcacctcct ggtacaacgc atccatcagc tcgccataca cgtcgagctg aacctgcgtg
     1921 ctggcggcgt tgccgacccg caccgggctg gcgccgcggt agccggccag ccagggaacc
     1981 tcggattcga gaatccagcg ctcgccggac agccggtaca gcgtctgcac ctggtcgggc
     2041 gtgccagcga ccgtgcggcg cagccacttg ccccaggcct gggcttcctc gcggtagccg
     2101 gcatgcatca gcgcaagcag ggtgaaggtg gcgtcgcgca gccagcagaa ccggtagtcc
     2161 cagttgcgcg cgccgccggg ctgttccggc agcgaggtgg tcggcgccgc aacgatgccg
     2221 ccggtcggcg cataggtcag cgccttcagc gtgatcagcg aactgtgcac cgcctcccgc
     2281 cattcaccct gatgggtgca gcgccgggtc caggcagtcc accacgcttc ggtctgctgc
     2341 aacgctgcat cgggcccggg cggttccggc ggctcgatat gcgatggcgc atgcgtcatg
     2401 acgaagcgca cccgctgccc gctgcgcacc gtgaagcgcg ccttcgtcgt catgtcctcg
     2461 ccgtgcagcg gcacgtcgct gtgcaccacg acctgatggc gccccgccac cgcacgcacg
     2521 ccgttgcggt gcggcaggcg ggtgacccaa gggatggcgg agccgtattc gaagcgcaac
     2581 gcgagatcga acgccatcgc gacgtgaccg gaccggcact ccagaatgcg cacgacggaa
     2641 tgggcgttcg gcaccatgaa gtcgagcagc gccacgctgc cgtgcggcgt ctcgaacacg
     2701 gtttccagga tcagcgtgcc cggccggtag cgccgatgca ccgcgccggg cgcgtcctgc
     2761 ggcgcgatct gccagcggcc attgcgcggc gtgcccagca gggcggcgaa gcaggcgcca
     2821 tcgtcgaagc gcggccagca cagccagtcg atggagcccg ccttgctgac cagggcggcg
     2881 gtctcacagt cgccgatcag cgcatagtcc tcgatcggcg ggggccgctc ggatcgatcc
     2941 ggcacatccg ggctggagcg cgggttggcg tccgaaacag tgccggcatg atccgtgcgg
     3001 ccgcgcgcca tcagcttgcg tgaccccaga cctgggtgcc gttcaccacg caatagcagc
     3061 cgctgcctgg agaaaccggg cggtccatcg ggcagacata agcgccggca tagcaggact
     3121 ggccggtgcc ggcctggtac ggggcatagc cctgcggcgg cgcgtagacc ggaggcggcg
     3181 cgtaatacac cggcggcgga taatacaccg gcggcggcgg cgcatagacc ggcggaccaa
     3241 aatacagcgg catgccgaat ccaacgaaca cgcgggcctg cgcggtggcc agaccggcgg
     3301 agacgaccag cccggcgccc atcgctgcca gaccgatcgt acgaagcgtg tgcttcggaa
     3361 gatttttgat catgggtgaa atcctggcaa acgaatgtat ccagatcatg acagagcaat
     3421 tttatcccgc aaacatcgat cgccattcac gcatgcgtgc ccgtgcgttt catcacggcc
     3481 agtgcgtcca gcgcccgcct gcgcccggcg gcgagatcga tcagcgggcg cggatacgcg
     3541 tgccccagcc ggatgccagc acgcgccagg tctgattccg gcacgtccca cggcgcatgg
     3601 atcgcggacg ggctcagccc ggccagttcc ggcacgaagc gccgcacata gtcaccctgc
     3661 ggatcgaatt tccgcccctg cagcaccggg ttgaagatgc ggaaataggg cgcggcatcg
     3721 gcgccgcttc cggcgaccca ctgccagccg gcggcattgt tcgccagatc ggcatcgacc
     3781 agcgtgtccc agaaccacgc ctcgccctgc tgccagggca gcagcaggtg cttcaccagg
     3841 aagctggcgg cgatcatccg cacccggttg tgcatccagc cagtctgcca gagctgccgc
     3901 atgccggcat cgacgatcgg gatgccggtc cgcccgcgct gccaggcgcg cagcgccggc
     3961 tgatcgtcgc gccagggcat gcgcgcgaag tcgggcagca gcggcgtttc cggcaatgcc
     4021 gggtggtgcc agagcaggtg ataggcgaac tcccgccaca gcacctcgcg ccgaaacgcg
     4081 gcgcggccct ccgcctcggg cagcgcgtgc cacaattgca ccggcgagat ctcgccgaag
     4141 cgcaaatgcg gcgacagcat ggaggtgccg gccgcgccag ggaggtcgcg cgcctcgctg
     4201 taggccggca gcgcgtcgcg gatgaagcgg tgcagccggt cctgcgcgcc ggcctcgccc
     4261 ggaacccagg ccgcccgcaa gccacccgcc cagtccggcc gtggcagcag gtcccacgcc
     4321 tccagccggt cggaagccgg cggcggcgga caggggatcc gctcggggcg tggatacgcc
     4381 gcctcgggaa cacccagttc caggcagcgg cgggagaacg gggtgaacac gccgaacggc
     4441 gtgccggacc tggtcttcac cgcctccggc tggaacagcg tggcggtgcg catccggtgc
     4501 agcgccacgc cctgcttcgc cagctccgcc gccagcgcgg cgtacgcttc gcgcgcccag
     4561 ggttcgta
//
LOCUS       DASSZH010000620         2457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_202833, whole genome shotgun sequence.
ACCESSION   DASSZH010000620 DASSZH010000000
VERSION     DASSZH010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202833"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VFN42_13395"
     CDS             complement(<1..339)
                     /locus_tag="VFN42_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997656.1"
                     /translation="MRDLYHACCGISDVAYSRYSTSPDRLGRMAPLDPTDAALRLAPE
                     IPMAREQTRTQVDDGDSGKSTFRVIERGLVAAMPAGKAGKIGSTILETGNRAARANSC
                     CRLFARALSDR"
     gene            361..1692
                     /locus_tag="VFN42_13400"
     CDS             361..1692
                     /locus_tag="VFN42_13400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET8997657.1"
                     /translation="MAPRVDPVASDTAPPPRSSVVVIGGGIIGCSTALFLARKGIPVV
                     LCEKGEIAGEQSSRNWGWCRKMGRDLREIPLAIEALRLWSEMNTLVEAETGFRQCGMV
                     YLCKTQKELEHREAWLDQAARPYQLDSRMLSKTEAAALFPGLAGDWAGGLYTPSDGRA
                     EAQKAAPAIATAARRAGATVLTQCAVRGVETQGGRIAGVVTERGRIACDSVVLAGGVW
                     SRLFCGSLDLRLPQLKVMSSVMRTHPIEGGPEISCAGFGFGVRKRLDGGYNLASWSGN
                     VHEIVPDTLRFFTDFLPAARMEWDTLRLRLGPAFLEEWRRPTRWSLDAETPFERVRVL
                     DPPPYEPILRQARESVTAAFPAFRDMRVAESWGGVIDVTPDAVPVISGVDTLPGFFIA
                     TGFTGHGFGIGPGAGRLMADLVTGAAPVVDPTPFRFSRFSDGSRPRPSPLA"
     gene            complement(1702..>2457)
                     /locus_tag="VFN42_13405"
     CDS             complement(1702..>2457)
                     /locus_tag="VFN42_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET8997658.1"
                     /translation="LDMLAARASRLRELRLRIRMVFQDPHASLNPRRSIGDSVAEAGD
                     INGTFRSRADRAARIAETLRSVGLDASFAARYPHELSGGQRQRVGIARALILQPRFVV
                     ADEPVSALDVSVQAQIVNLLEQLRTRFGLAMLFVSHDLAVVGHISDRIAVMYLGRVVE
                     LGSAARVIGAPAHPYTEALLSAVPAIRSGAAKRIVLSGEMPSPVHPPSGCAFRTRCPY
                     AIAACAEQHPSLHQRDAGHYTACLRADLTLRGA"
BASE COUNT          415 a          804 c          843 g          395 t
ORIGIN      
        1 cctgtcagac agcgcccgcg caaataaccg acaacacgaa tttgctcgcg cggcgcggtt
       61 gccggtttcg aggatcgtgg aaccgatctt cccggcttta ccggccggca tcgccgcgac
      121 gagaccgcgc tcgatgacgc ggaacgtgct cttgccgctg tcgccgtcat ccacctgcgt
      181 cctagtctgc tcgcgcgcca tcgggatttc cggtgccagc cgcagcgccg cgtcagtggg
      241 atccaacgga gccatccgcc caagcctgtc cgggctggtg gagtatctgc tataagcaac
      301 atccgaaatg ccgcaacagg cgtgatacag atcgcgcacc agaaggagga atcctgaccg
      361 atggcgcctc gcgtagatcc cgttgcctct gataccgccc cgcctccgcg cagcagcgtg
      421 gtggtgatcg gcggcggaat catcggctgc agcaccgcgt tgttcctcgc ccgcaagggc
      481 attcccgtgg tgctgtgcga aaagggtgag atcgccggcg agcagtccag ccgcaattgg
      541 ggctggtgcc gcaagatggg ccgcgatctc cgggaaatcc cgctggccat cgaggcgctg
      601 cggctgtggt ccgaaatgaa cacgctggtg gaagcggaaa ccggtttccg ccagtgcggc
      661 atggtctatt tgtgcaagac acagaaggag ctggaacacc gggaagcgtg gctggatcag
      721 gccgcgcggc cgtaccagct ggacagccgc atgctcagca agacggaagc cgccgcgctg
      781 tttcccgggc tcgccggcga ctgggcgggc ggcctgtaca cgcccagcga cgggcgcgcc
      841 gaggcgcaaa aggcggcgcc tgctatcgct actgccgcgc gccgcgccgg tgcgacggtg
      901 ttgacgcaat gcgcggtgcg tggcgtggag acacagggcg gacgcatcgc cggggtggtg
      961 accgaacgtg gccgcatcgc ttgtgattcg gtggtgctgg cgggcggcgt ctggtcgcgg
     1021 ctgttctgcg gctcgctcga cctgcggctg ccgcaactga aagttatgtc gtcggtaatg
     1081 cgtacgcatc cgatagaggg cggcccggaa atctcgtgcg ccggcttcgg cttcggcgtg
     1141 cgcaaacggc tcgatggcgg ctacaacctc gcgagctgga gcggcaacgt ccacgagatc
     1201 gtgccggata cgctgcgctt cttcacggat ttcctgccag cggcacgcat ggaatgggac
     1261 acgctgcggc tgcgcctggg gccagcgttc cttgaggagt ggcggcggcc cacgcgctgg
     1321 tctttggatg cggaaactcc gttcgaacgg gtccgggtgc tcgatccgcc gccctatgaa
     1381 ccgattctgc gccaggcacg cgaatccgtc accgcggcct tcccggcttt ccgcgacatg
     1441 cgggtggcgg aaagctgggg cggcgtgatc gacgtgacac ccgacgcggt gccggtgatc
     1501 tccggcgtgg acacgctgcc cgggttcttc atcgcaactg gcttcaccgg acatggattc
     1561 ggcatcgggc caggcgcagg ccggctgatg gcggatctgg tcaccggcgc ggcgccagtg
     1621 gtcgatccca cgccgttccg gttcagccgc ttcagcgacg gcagccgacc ccgcccatcg
     1681 ccgctggctt agccccctgc gtcacgcgcc acgcagagtc aggtcggccc gcaggcaggc
     1741 ggtgtaatgg ccggcgtccc gttggtgcag tgacggatgc tgctccgcgc aggcggcaat
     1801 cgcgtaggga cagcgcgtgc ggaacgcgca tccgctgggc ggatgcaccg ggcttggcat
     1861 ctcgcctgac aggacaatgc gttttgccgc gccggacctg atcgccggaa ccgctgaaag
     1921 caacgcctcg gtatagggat gcgccggtgc gccgatcacc cttgccgccg aacccagctc
     1981 gacgacgcgt ccgagataca tcaccgcaat gcggtcggag atgtggccaa ccactgccag
     2041 gtcatgcgag acgaacagca tcgccaaccc gaacctggtg cgcagctgct ccaacaggtt
     2101 gacgatctgc gcctgcaccg acacgtcgag cgcagaaacc ggctcgtccg ccaccacgaa
     2161 acgcggttgc aggatcaagg cccgcgcaat gccgacgcgc tggcgctgac cgccgctgag
     2221 ttcgtgcggg tagcgggcgg caaatgatgc atcgagaccg accgagcgaa gcgtctccgc
     2281 gatacgcgcg gcgcgatcgg cgcggctgcg aaaggtccca ttgatgtcgc cggcctcggc
     2341 cacggaatcg ccgatcgaac ggcgcggatt gagcgatgca tggggatcct ggaagaccat
     2401 gcgtatccgc aagcgcagct cgcgcagcct tgacgcgcgc gccgcaagca tgtcgag
//
LOCUS       DASSZH010000621         2458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_850164, whole genome shotgun sequence.
ACCESSION   DASSZH010000621 DASSZH010000000
VERSION     DASSZH010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850164"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1484)
                     /locus_tag="VFN42_13410"
     CDS             complement(54..1484)
                     /locus_tag="VFN42_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8997659.1"
                     /translation="MSRFLPRTLFARMLLILLAGLAVSHLVGTLIYASDRAQAVRAIG
                     AYAATQRIANLTQLIDEAPPDWRSRIVRAASDPSLRVAVSAARPAFAEQSGDANSLTV
                     RHYLADQLPAGLADRLQVAVNAAPENFAGFDPHASMNMAHMMRGMSMRGPMTMAAPPW
                     LQGAGGLRSLRAAVQLSDGAWLAFATALPNAAPPTPWPFVAATATMAVIVVLASAWAV
                     GRVTAPLRLLAQAAERLGRDVNAPPIAEAGTREVRQATQSFNQMQARIRRLVDNRTRM
                     LAALSHDLRTPLTLLRLRTEGLPETEDRERMLATIGELDTMIGASLNFARDQSMAEVT
                     RRTDVGALLTSIVDDMADAGLPVTMEPADSVVLDCQPGALRRALTNLIENAVKYGGSA
                     QVGIAATASGVKIDVEDNGPGIPEAELQRVFEPFHRLEESRSRETGGTGLGLSIAQSI
                     VQAHGGDIVLANRRGGGLRARITLPR"
     gene            complement(1481..2203)
                     /locus_tag="VFN42_13415"
     CDS             complement(1481..2203)
                     /locus_tag="VFN42_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8997660.1"
                     /translation="METSPHILIVDDHREIRELVGRALTKEGFRVTAVGDGKAMRQAM
                     ADSRIDLILLDLMLPGEDGLSLCRWVRADSDVPIIMLTAKGEEVDRVIGLEMGADDYL
                     PKPFGSRELIARIHAVLRRTRHAPAASDPPPESQNYSFDRWVLNTESRELLRDDSVTV
                     PLSTGEYELLLALVERPQRVLSRDQLLDLARGRAASVFDRSIDTQVSRLRKKLERDPA
                     DPHIIKTIWGGGYMFAASVTRT"
     gene            complement(2179..>2458)
                     /locus_tag="VFN42_13420"
     CDS             complement(2179..>2458)
                     /locus_tag="VFN42_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997661.1"
                     /translation="VRLTPPIANTVAITAKPLLIFLIGVALLFRDGRTLPVTCYLDRS
                     CHRSLSPAGKVVPSCGNGRGLPHPVTHWLCRIAAESPILGAWKLRRTF"
BASE COUNT          407 a          835 c          818 g          398 t
ORIGIN      
        1 cgaagccgga gactattgcg aaatggctga cggcctgaac gcgcccattt ggatcaacgt
       61 ggcagtgtga tcctggcacg caaaccaccg ccacggcggt tcgccagcac gatgtcgccg
      121 ccatgcgcct gcacgatgga ctgcgcgatc gacagaccca ggccggtgcc gccggtttcc
      181 cggctgcgcg attcctccag ccgatggaac ggttcgaaca cccgttgcag ctccgcctcc
      241 ggaatgccag ggccgttgtc ttcgacgtcg atcttcacgc cggacgcggt cgcggcaatc
      301 ccgacttgcg ccgagccgcc gtacttcacc gcgttttcga tcaggttggt cagcgcccgg
      361 cgtagcgcac cgggttggca gtccagcacc accgaatccg ccggctccat tgtcaccggc
      421 agcccggcgt ccgccatatc gtccacgatg gaagtcagca gcgcgcccac atcggtgcgt
      481 cgcgtcacct cggccatcga ctggtcgcgg gcgaagttca gcgacgcgcc gatcatcgta
      541 tccagctcgc cgatcgtcgc cagcatgcgc tcgcggtcct cggtttccgg caggccctcg
      601 gtacgcagcc gcagcagcgt cagcggcgtg cgcagatcgt gcgacagcgc cgccagcatg
      661 cgggtgcggt tgtccaccag ccggcggatg cgtgcctgca tctggttgaa cgactgcgtg
      721 gcctggcgca cctccctggt tcccgcttcg gcgatcggtg gcgcattcac gtcacggccg
      781 agccgctcgg cggcttgcgc gagaaggcgt agtggcgcgg taacgcggcc gaccgcccag
      841 gccgaggcaa gcacgacgat caccgccatc gtcgccgtcg cggcaacaaa cggccagggc
      901 gtcggcggcg cggcgttcgg caatgccgtc gcgaaggcca accacgcccc atcggacaat
      961 tgcaccgcgg cgcgcaggga gcgcaatcct cccgcaccct gcaaccaggg cggcgccgcc
     1021 atggtcatcg gccccctcat cgacatgccg cgcatcatgt gcgccatgtt catggacgcg
     1081 tgcgggtcga agccggcgaa gttttccggc gcggcattaa cagccacttg cagtcgatcc
     1141 gccagtcccg ccggcagctg gtcggcgagg tagtgccgga ccgtcagcga attcgcgtca
     1201 ccgctctgct cggcgaaggc ggggcgggcg gccgacacgg cgacgcgcag actgggatcg
     1261 ctggccgccc gcacgatgcg gctgcgccag tcaggcggcg cttcgtcgat cagctgtgtc
     1321 aggttggcga tgcgttgcgt tgccgcatag gcgccgatcg cgcgcaccgc ctgtgcccgg
     1381 tcagaagcgt agatcagcgt gccgacgaga tgcgacacgg ccagcccggc cagcaggatc
     1441 agcagcatcc gggcaaacag cgtgcgcggc agaaagcggc tcatgttcgg gtcacgctgg
     1501 cggcgaacat gtagccgccg ccccagatcg tcttgatgat gtgcggatcg gccggatcgc
     1561 gctccagctt cttgcgcaag cggctgacct gcgtgtcgat gctgcggtcg aacacgctgg
     1621 ccgcccgccc gcgcgccaaa tccagcagct ggtcgcggct cagcacgcgc tgcggccgtt
     1681 cgaccagcgc cagcagcagc tcgtactcgc cggtgctgag tggcacggtg acgctgtcgt
     1741 cgcgcaacag ctcacgcgat tcggtattca gcacccagcg atcgaaactg tagttctgcg
     1801 actcgggtgg tggatcggag gcagccggtg catggcgggt gcgccttagc accgcgtgaa
     1861 tacgggcgat cagttcgcga ctgccgaagg gcttcggcag gtagtcgtcc gcgcccatct
     1921 ccaggccgat gacgcgatcc acctcttcac ccttggccgt cagcatgatg atggggacat
     1981 cgctgtcggc cctgacccag cggcacagcg acagaccgtc ctcgcccggc agcatcaggt
     2041 cgagcaggat caggtcgatg cgggaatcgg ccatcgcctg ccgcatggcc ttgccgtcgc
     2101 ccactgcggt gacgcggaat ccctccttgg tcagcgcgcg tccgaccagt tcgcggattt
     2161 cccgatggtc gtcgacgatc agaatgtgcg gcgaagtttc catgcgccca aaataggcga
     2221 ttcagccgca atgcggcaca gccagtgtgt gacagggtgt ggcaagcccc ggccattgcc
     2281 acaactgggc acaactttcc cggccggtga caaactccgg tgacagctgc ggtccagata
     2341 gcaggtgacg ggcagggttc ggccgtcgcg gaacaggagg gccactccga tgaggaagat
     2401 cagcaagggt ttggcggtga tcgccacggt gttggcgatc ggtggcgtca gcctgacg
//
LOCUS       DASSZH010000622         2921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1583708, whole genome shotgun sequence.
ACCESSION   DASSZH010000622 DASSZH010000000
VERSION     DASSZH010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1583708"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VFN42_13425"
     CDS             complement(<1..626)
                     /locus_tag="VFN42_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HET8997662.1"
                     /translation="MVDLLCMGEPMLEFNQLPPLPDGSVHYLEGHGGDTSNAAVAAAR
                     QGASVGYITAIGTDAPGDSFMAMWAREGVDTGTVIRTDRALTGVYFVTHDAAGHHFLH
                     YRKDSPAALYGPADVPHAALAAARMFYVSGISQGISPSAADAVFAAIDTCRRNGVAVA
                     YDTNYRPRLWPPVRAAAVIHAAMALADYAFPGMDDALVLTGLADPDAIL"
     gene            complement(626..1249)
                     /gene="eda"
                     /locus_tag="VFN42_13430"
     CDS             complement(626..1249)
                     /gene="eda"
                     /locus_tag="VFN42_13430"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HET8997663.1"
                     /translation="MPESLIDRFRAARVVPVVRTQSTQHAATAVQWLREAGLRIFEIT
                     MTVPDAPALIRDLAADPDLLVGAGTVPDRATADACLAAGARFIVAPWVDASLSAPCRA
                     ADAELMLGALTPNEVRAAQAAGAGVVKIFPASSIGGPAHVRALRSVFPNVLFCPTGGV
                     DPGNAAAYLQAGAAFVGIGGALVDEKRIAAGDRTAIQDAARQVLEIA"
     gene            1358..1762
                     /locus_tag="VFN42_13435"
     CDS             1358..1762
                     /locus_tag="VFN42_13435"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8997664.1"
                     /translation="MSQAATMTKRLLADEPSPPSVYVDVANAPWETSKFPGIQHKIMY
                     SDDGGLAAILFKLAPGAVVPLHEHTGIEMTYVLEGSLQDEEGVCTAGNFVWRPAGNTH
                     EAVAPNGAVILGVFMKPNHFAAGQKFFTQQAG"
     gene            complement(1820..2257)
                     /locus_tag="VFN42_13440"
     CDS             complement(1820..2257)
                     /locus_tag="VFN42_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010513882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8997665.1"
                     /translation="MIDLRDAAADAASVIRALQLQPHPEGGHYREAWRDAPAEGGRGA
                     GTAILFLLRAGERSAWHRVDAAELWLWQAGAPLTLRVAPQTTLRLGPDLRAGDKLQGL
                     VPAHQWQAAESLGPWTLCTCVVAPAFDFAGFELATGAIPFAAG"
     gene            complement(2254..2853)
                     /locus_tag="VFN42_13445"
     CDS             complement(2254..2853)
                     /locus_tag="VFN42_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HET8997666.1"
                     /translation="MKLFYSPTSPYVRKVMACAITRGIDDRIELLRVNANASPPELLA
                     ANPLSKVPCLITEDGVALFDSPVICEFLDTIGDALPTMVPEGGGARWRALKQQAMGDG
                     ILDASVPRRAELQKPQDEGRVAWIARQKAAVDRTLDALERDLPQDYPDIGSIAVACAL
                     GYLDFRYAAEPWRPNRPRLAAWFESFAENPGIAQTVPRE"
BASE COUNT          469 a         1056 c          982 g          414 t
ORIGIN      
        1 aggatcgcat ccgggtcggc gagtcccgtc agcaccagcg cgtcgtccat gcccggaaag
       61 gcatagtcgg ccaacgccat ggcggcatgg atcaccgcgg cggcgcgcac cggcggccac
      121 agccgcgggc ggtagttggt atcgtacgca accgccaccc cgttgcgccg gcaggtgtcg
      181 atcgcggcga acaccgcatc cgccgcgctg ggggaaatcc cctggctgat gccggacaca
      241 tagaacatcc gcgccgccgc cagcgccgcg tgcggcacgt ccgccggccc gtacagcgcg
      301 gccggagaat ccttgcggta atgcaggaaa tggtgccccg cggcgtcatg cgtgacgaaa
      361 tacaccccgg tcagcgcgcg gtcggtgcgg atcaccgtgc cggtatcgac gccttcccgc
      421 gcccacatcg ccatgaagct gtcgcccggc gcgtccgtgc caatcgcggt gatgtagccc
      481 accgacgcgc cctgccgcgc tgccgccacc gcggcattgg aggtgtcgcc gccatggcct
      541 tccaggtagt gcacgctgcc gtccggcagc ggcggcaact ggttgaactc cagcatcggc
      601 tcgcccatgc acagcaggtc caccatcacg cgatctccag cacctggcgc gcggcgtcct
      661 ggatcgcagt gcggtcgcct gccgcgatgc gcttctcgtc caccagcgcg ccgccgatgc
      721 cgacgaaggc cgcgccggcc tggaggtacg ccgcggcatt gccgggatcg acgccgccgg
      781 tgggacagaa cagcacgttg ggaaacacgc tgcgcagcgc ccggacatgc gccgggccgc
      841 cgatcgagga agccgggaag atcttcacca cgccggcgcc ggccgcctgc gccgcgcgca
      901 cttcgttcgg cgtcagcgcg cccagcatca gctcggcatc cgcggcgcgg cagggcgcgc
      961 tcagtgatgc gtccacccag ggcgccacga tgaagcgcgc gcccgcggcc aggcacgcgt
     1021 ccgccgtggc gcggtccggc accgtgccgg cgccgaccag cagatccggg tcggcggcca
     1081 ggtcgcggat cagcgccggg gcgtccggca ccgtcatggt gatctcgaag atgcgcagcc
     1141 ccgcctcgcg cagccactgc accgcggtgg ccgcatgctg ggtggactgg gtgcgcacca
     1201 cgggcaccac gcgcgcggcg cggaagcggt cgatcagcga ttctggcatg gcgtttctcc
     1261 cgtgctgccc ggtcaccaca agcgccttcc gcttgacagc gcaagccccg atcaggccgc
     1321 atgtcgggcg ctatcacccc tgggaggaga accggccatg tctcaagccg cgacgatgac
     1381 caagcgcctg ctggcggacg aaccctcgcc gccttcggtg tatgtcgatg tggcgaacgc
     1441 gccgtgggag acatcgaaat tcccgggcat tcagcacaag atcatgtatt cggacgatgg
     1501 cggcctggcc gcaatcctgt tcaagctggc gcccggcgcg gtggtgccgc tgcatgagca
     1561 caccgggatc gagatgacct acgtactgga gggcagcctg caggatgagg aaggcgtgtg
     1621 caccgcgggc aattttgtct ggcgtccggc gggcaacaca cacgaggcgg tggcgccgaa
     1681 cggtgcggtg atcctgggtg tgttcatgaa gcccaaccac ttcgcggcag ggcagaagtt
     1741 cttcacgcag caggcgggct agccgcgcgg ctgaagcgca ggccggcgcc tgtgcgtcac
     1801 ggccggcgaa gggcttgatt taccccgccg caaacggaat cgcgccggtc gccagctcga
     1861 atccggcgaa gtcaaaggcc ggcgccacca cgcacgtgca cagcgtccag ggccccaggc
     1921 tttctgccgc ctgccactga tgcgccggca ccagcccctg cagcttgtca cccgcgcgga
     1981 ggtccggtcc cagacgcaac gtggtttgcg gcgcgacacg cagcgtcagc ggcgcgccgg
     2041 cctgccacag ccagagttcc gcggcgtcaa cccgatgcca ggcgctgcgt tcaccggcgc
     2101 gcagcaggaa caggatcgcc gtccctgccc cgcgcccgcc ctcggcaggg gcgtcccgcc
     2161 aggcctcgcg gtaatgccca ccctccggat gcggctgcaa ttgcagcgcg cggatcaccg
     2221 atgccgcgtc agcggcggca tcgcgcaggt cgatcattcc cgcgggacgg tttgagcgat
     2281 gcccgggttc tccgcgaagc tctcgaacca cgccgccaac cgcgggcggt tcggccgcca
     2341 gggctccgcg gcatagcgga aatccaggta gcccagcgcg caggcgacag cgatggaccc
     2401 gatatccgga tagtcctgcg gcaggtcccg ctccagcgcg tccagcgtgc ggtccaccgc
     2461 cgccttctgc cgagcgatcc aggcaacgcg accttcatcc tgcggcttct gcagctccgc
     2521 gcggcgcgga accgaggcat ccaggatgcc gtcgcccatc gcctgctgct tcagcgcacg
     2581 ccagcgcgcc ccgccaccct ccggcaccat ggtcggcagt gcatcgccga tggtgtccag
     2641 gaattcgcag atcaccgggc tgtcgaacag cgccacgccg tcttccgtga tcaggcaggg
     2701 cactttggac agcggattgg cggccagcag ttccggcggc gaggcattcg cattgacacg
     2761 cagcagttcg atccggtcat caatgccgcg ggtgatggcg caggccatca ccttgcggac
     2821 gtatgggctg gttggcgagt agaacagctt cacccgaaat ccccctcaga acctgtcttt
     2881 ttcggcgcgc aacgcggcga agcgcgccac atccggtgca c
//
LOCUS       DASSZH010000623         3132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1429805, whole genome shotgun sequence.
ACCESSION   DASSZH010000623 DASSZH010000000
VERSION     DASSZH010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429805"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VFN42_13450"
     CDS             complement(<1..439)
                     /locus_tag="VFN42_13450"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8997667.1"
                     /translation="MSEPQTFTFADDGAIPNSLLPLLVYRNAVPPDADAIEARFARNR
                     WPPAWRDGVFPFHHFHSNAHEVLGVARGWVKVRFGGPGGQTVQVEAGDVAVLPAGVGH
                     CNEGQSPDLLIVGAYPDNGPSPDTCRGKPDEHDAVLRNVAATPC"
     gene            complement(754..1293)
                     /locus_tag="VFN42_13455"
     CDS             complement(754..1293)
                     /locus_tag="VFN42_13455"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HET8997668.1"
                     /translation="MNNTTKLTATALAVMLAGAPVGAFAWTGTQSNNGAPSQSPNVNN
                     TAPARSGGYAPAASTGSQGYGQTASGGSQAMNTPSSAQGSDMSMQGGQMGTQPGTQGN
                     RQVSQNTVAAVQQKLQREGLYQNGRVDGRMGPKTHTALESFQHSHGLAPNGRLDGQTL
                     AALGIDQHSTSDMNAPGNG"
     gene            complement(1433..2446)
                     /locus_tag="VFN42_13460"
     CDS             complement(1433..2446)
                     /locus_tag="VFN42_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8997669.1"
                     /translation="MSQTPPVQPVLQVNDLVVHFPVVGAARGSVVHALDGVSFTVERG
                     RSFGLVGESGSGKSTAALAVMRLVPATAGAVMLDGADFLALRGKALRQARRHIQMIFQ
                     DPFSSLDPRRRAGELIAEPIRLLEGVSRRAAAERVPPLLAAVGLPADAASLFPHQFSG
                     GQRQRLCIARALATEPELIVCDEAVSALDVAIQAQILNLLKRLQAERGLAYIFISHDL
                     GVVQYVCDDVAVMYLGQIVEQAPADLFFSHPRHPYSWSLISAAVPAGAARDALRRRHL
                     VRGEPPSPIDLPPGCRFAARCPFATDRCRREEPALHPIASGHFAACHRINEIEPPSFG
                     AVD"
     gene            complement(2487..>3132)
                     /locus_tag="VFN42_13465"
     CDS             complement(2487..>3132)
                     /locus_tag="VFN42_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8997670.1"
                     /translation="KQAVEMLRLVRIPEPARRAQEYPHQLSGGMRQRVMIAMALACRP
                     QLLIADEPTTALDVTVQAEVFDLLQQLQQEMGTAILLITHDLGTVADIADTVAVLYAG
                     RCVEQGATRQVLETPLHPYSAALMAATPHLRLGAAAADRRPALLEIPGLVPPLGQRAS
                     ICAFAGRCPHADAPCLERLQPGLTEHYPGHTVACWHPGALAGADSALVAGEAGT"
BASE COUNT          520 a         1084 c         1048 g          480 t
ORIGIN      
        1 gacagggtgt ggcggcgacg ttgcgcagga ccgcatcgtg ttcgtccggt ttgccgcgac
       61 aggtatcggg cgaagggccg ttgtccggat aggcgccgac gatcagcagg tccggcgact
      121 gcccctcgtt gcaatgcccg acgccggccg gcaacacggc gacatccccg gcctccacct
      181 gcacggtctg gccgcccggg ccgccgaaac gcaccttcac ccagccccgc gcgacaccca
      241 gcacctcatg cgcattgctg tggaagtggt ggaacgggaa cacaccgtca cgccacgccg
      301 gcggccagcg gttccgtgca aagcgggcct cgatggcatc ggcgtccggc ggcacggcat
      361 tgcgatatac cagcagcggc agcagcgagt tcgggatcgc gccgtcatcg gcgaaggtga
      421 aggtctgcgg ttcgctcatg cttgcctccg gtgcaatcag gccagcttca ttctaccggt
      481 atacggccgg gaatggatgc tcaacggact gcgacaccag ccaacttacg gcggcgcccc
      541 tctcatacca gcggcccgaa gttctaaccc atccgtgtca tggccgggct tggcccggct
      601 acccacgtct ttgccgcatg catcacagta ggacgtgggt gcccgggcca agcccgggca
      661 tgacacaaac gagtccgggc ttcgggccgc tggtatcaaa cgaaaagagg cgccgggctg
      721 ctcgcccgac gcccctccgc gatgatcgct ggatcagccg ttgcctgggg cattcatgtc
      781 ggaggtggag tgctggtcga tccccagcgc ggccaaggtc tgcccgtcca gccggccgtt
      841 cggcgccaag ccatgcgaat gctggaagct ctccagtgcg gtatgggtct tcggccccat
      901 ccggccgtca acacgaccgt tctggtacag cccttcccgc tgcagcttct gctgaaccgc
      961 ggcaaccgtg ttctgcgata cctgccggtt gccctgcgtg ccgggttgtg tgcccatctg
     1021 gccgccctgc atgctcatgt cactgccctg cgcgctggac ggtgtgttca tcgcctgcga
     1081 accgccggac gcggtctgac catagccctg gctgccggtg gacgccgccg gggcatagcc
     1141 tccagaacgc gccggagccg tgttgttgac gttcgggctc tggctcgggg caccgttatt
     1201 ggactgggtg ccggtccagg caaatgcgcc aaccggcgcg ccggcaagca tcacggccag
     1261 tgcggtcgcg gtgagtttcg tcgtgttgtt catcccgtcc tcctttcggc ttgttccgag
     1321 cgacggcaag ccgccgcctc cgggataccc gccaagtgga cccgctatgg cggaatgcca
     1381 ctcaggagaa cgggaagaac aatggttcgc agccccgtcc tgcgggccac agtcagtcca
     1441 cggctccgaa gcttggcggt tcaatctcgt tgatgcggtg acaggcagcg aaatggcctg
     1501 acgcaatcgg atgcaacgcg ggttcctccc tgcgacagcg gtccgtcgcg aaaggacagc
     1561 gggcagcgaa ccggcagccc ggcggcagat cgatcgggct cggcggttct ccgcgcacca
     1621 ggtggcggcg gcgcaatgca tcacgcgccg caccggcggg aaccgctgcc gaaatcagcg
     1681 accagctata gggatggcgc gggtgcgaga agaacagatc cgctggcgcc tgttcgacga
     1741 tctgcccgag atacatcact gcgacgtcgt cgcagacgta ctgcaccaca cccaggtcgt
     1801 gcgagatgaa gatgtaggcc aggccgcgct ctgcctgcag ccgcttcagc aggttcagga
     1861 tctgcgcctg gatggccacg tccagcgccg agaccgcctc gtcgcagaca atcagttccg
     1921 gttccgtggc gagcgcacgc gcgatgcaaa gacgctggcg ctggccgccg gagaactggt
     1981 gcgggaacag gctcgcggca tccgccggca agcccaccgc ggccagcaga ggcggcaccc
     2041 gttcggccgc cgcgcggcgg gatacgccct ccagcaatcg gatcggctcc gcgatcagtt
     2101 ccccggcccg ccgccgcgga tcgagcgacg agaagggatc ctgaaagatc atctggatgt
     2161 gccggcgcgc ctgacgcaac gccttgccac gcaaggcgag aaaatccgcg ccgtccagca
     2221 tgaccgcgcc ggcggtcgcc ggcaccaggc gcatgaccgc cagggccgcg gtggacttgc
     2281 ctgatccgga ttcgccgaca aggccgaagc tccgcccgcg ctccaccgtg aagctgacgc
     2341 cgtccagcgc atgcacgacc gagccgcgcg cggccccgac caccgggaaa tgcacgacaa
     2401 ggtcgttcac ctgaagcacg ggctgcaccg ggggagtctg gctcatgcgg gcggcagggg
     2461 ccggttggcc caccagcgga ccaggatcat gtgccggcct cccccgccac cagggcggag
     2521 tccgccccgg cgagcgcacc gggatgccag caggccaccg tgtgacctgg ataatgctcc
     2581 gtcaggccgg gttgcagccg ctccaggcat ggcgcgtcgg catgcggaca gcgacctgca
     2641 aaggcgcaga tgcttgcgcg ctgtcccagc ggcggcacca gcccgggtat ttccagcagc
     2701 gccggccggc ggtccgcggc cgccgcaccc aggcgcagat gcggcgtcgc ggccatcaac
     2761 gcggcagaat agggatgcag cggcgtctcc agcacctgcc gggtggctcc ctgctcgacg
     2821 cagcggccgg cgtacagcac cgccaccgtg tcggcgatgt cggcgactgt gcccaggtca
     2881 tgggtgatca gcaggattgc ggtgcccatc tcctgctgaa gctgctgcag caggtcgaac
     2941 acctcggcct gtactgtgac atccagcgcg gtggtcggct cgtcggcaat cagcaattgc
     3001 ggccggcagg ccagggccat ggcaatcatc acgcgctgcc gcatgccgcc ggagagctgg
     3061 tgcggatact cctgggcgcg tcgcgccggc tccggaatgc ggaccagccg cagcatttcg
     3121 acggcctgct tc
//
LOCUS       DASSZH010000624         2379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_509117, whole genome shotgun sequence.
ACCESSION   DASSZH010000624 DASSZH010000000
VERSION     DASSZH010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509117"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VFN42_13470"
     CDS             complement(<1..685)
                     /locus_tag="VFN42_13470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997671.1"
                     /translation="MSVTYVSGQTSSGLVIDRGSLLQVQAGGTIVDATFNGGVGFGEV
                     SALAVGISVNSGGFFDLQSGSFGSGIVVNSGGQAGADFGGSATDIAVNNGGTLSIDTS
                     GTASDVLVNSGGTFVVSAGGSASDVMLNGGTLDVLVGGSVSGAIDFVPGTGGTVVVSS
                     PASAGNAIPELTYSGFGPHDSIDLMGAFYTGSNTFAVSGSTVTLVVPLQGLPAQPFPM
                     IMENPQPGVE"
     gene            1327..2088
                     /locus_tag="VFN42_13475"
     CDS             1327..2088
                     /locus_tag="VFN42_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8997672.1"
                     /translation="MAQLHGKVAWVTGAASGIGEATALALAAEGATVVLTGRRVAPLA
                     DVARRIREAGGTAHVQAGDLGASAVVHRIADWIGGELQRLDILVNNAGLNITARAWRD
                     LQPEGVDAVVQANLSAAFYCAMAALRLMRPQRDGVLIHTASLAGRFVSPLSGPAYTAA
                     KHAVVAMSHSINMEECVNGIRSTAVCPGEVATPILDKRPIPVPPEERAKMVQPQDCAD
                     LIRYVACLPPHVCINEVLITPTWNRAYVAALERKL"
BASE COUNT          391 a          865 c          772 g          351 t
ORIGIN      
        1 attccacgcc cggctgcgga ttctccatga tcatgggaaa cggttgcgcc ggcagaccct
       61 gcagcggcac gaccagcgtg acggtgctgc ccgaaacggc aaacgtgttg gacccggtgt
      121 agaaggcccc catcaggtcg atgctgtcat gcggcccgaa gccggagtag gtgagttccg
      181 ggatggcgtt ccccgccgag gccggagagg acacgacgac ggtaccaccg gtgcccggca
      241 cgaaatcgat cgccccggac accgagccgc cgaccagcac gtcgagcgtg ccgccgttca
      301 gcatcacgtc ggaagcactc cccccggcac tcaccacgaa tgtgccgccg ctgttgacga
      361 gcacgtccga ggccgtgccg gaggtgtcga tcgacagcgt cccgccgttg ttgacggcga
      421 tgtccgtggc actgccgccg aaatcggcgc cggcctggcc gccgctgttg acgacgatgc
      481 cgctgccgaa gctgcctgac tgcaggtcga agaacccgcc ggaattgacg gagatgccga
      541 cggcaagggc gctgacctcg ccgaatccga caccgccgtt gaacgtcgcg tccacgatcg
      601 tgccgccggc ctgcacctgc agcagcgatc cgcgatcaat caccagtccg ctgctggtct
      661 gaccggagac gtacgtgacc gacataactc ctcccaccgc cggacggagg accgcctccg
      721 tatccgttac ggggatgcta accggccgcg gacaggcgtc gagatcgcgt tcgggatagc
      781 ggcggaggct ggcatggcga cataccgctt cggtgtcgcc gccgtcatga ccgcgcaccc
      841 ccacagtggc gcgtgacgag tggcatcaga caacatctct ctgcctgtct gcgtcgccag
      901 ctgacgcaca ggacgcggcg ggcggggcag gggcgccagg gccggggcga cagagccgga
      961 tgatctccgg aacggataac cgagaattta ggcaccttcc ctgttcaggg cgcggaccgg
     1021 aattcgagga cgccgggcac cggtgcattc gtcctgcagc gcctgcgccg gctcggccgt
     1081 gaggacgcga ttggccacgg ccctcggctc aggcagcccc tggcgtccag tgcagccctg
     1141 ccggcggctg cttcgtcccc acgtcaccga agagctccat ctggcgcacc ggcatctgtg
     1201 cgatcaggcg cgccatgtcg tccaccgcca gcggcagcgg acccgtttgc cccgcacaac
     1261 cgaggcgacg ttgaccgtgc aggtccgcgc tcccacactg cgccggctgc acgaagggtt
     1321 cggaagatgg cgcaactgca cggcaaggtc gcctgggtca cgggtgcggc cagcggcatc
     1381 ggcgaggcca cggcgctggc gcttgccgcc gagggcgcca cggtggtcct gaccggccgc
     1441 cgcgtcgccc ccctcgcgga tgtcgcccgc cgcatccgcg aggctggcgg caccgcccat
     1501 gtgcaggccg gcgatctcgg cgcgtccgcc gttgtccacc gtatcgccga ctggatcggc
     1561 ggcgaacttc agcggctcga catcctggtc aacaacgccg gcctgaacat caccgcccgc
     1621 gcctggcgcg acctgcagcc ggagggcgtc gatgcggtcg tgcaggccaa cctctccgcc
     1681 gccttctact gcgccatggc cgccctacgc ctgatgcgac cacagcgtga tggcgtgctg
     1741 atccacaccg cctcgctcgc cggccgcttt gtcagcccgc tcagcggccc cgcctatacc
     1801 gccgccaagc acgccgtcgt cgcgatgagc cacagcatca acatggagga atgcgtcaac
     1861 ggcatccgct ccaccgccgt ctgcccgggc gaggtcgcaa cccccatcct cgacaagcgc
     1921 cccatcccgg tcccgcccga ggagcgcgcg aagatggtac agccgcagga ctgcgccgac
     1981 ctgatccgct acgtcgcgtg cctgccgccg catgtctgca tcaacgaggt gctgatcacc
     2041 cccacctgga accgcgccta cgtcgccgcc cttgagcgca agctctgatc cgagccatac
     2101 aacaggtgcg gctctcgccc cgatccgatc agcctggaag gttgcaggac aatgggcgat
     2161 acgtgacacg atgaagctgc gcgtgcggca aggcggccaa gcatcaggtc tcccggtcag
     2221 gagctttacg atcacgtggg gcatctttcc gttctccatc gcgatgcgca ggcgccatcc
     2281 gcgtgcggtg gcatagtcgg gaaccgccgc gcgcgcccgg atgatcgcta tgcctcgccg
     2341 atctcgtcgg cgagccggta gccgacgccc ggctcggtg
//
LOCUS       DASSZH010000625         4252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_151171, whole genome shotgun sequence.
ACCESSION   DASSZH010000625 DASSZH010000000
VERSION     DASSZH010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151171"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /gene="aroC"
                     /locus_tag="VFN42_13480"
     CDS             complement(<1..596)
                     /gene="aroC"
                     /locus_tag="VFN42_13480"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET8997673.1"
                     /translation="MSHNSFGHLFRVTTWGESHGPAIGCIVDGCPPRLSLTEADIQPW
                     LDRRRPGQSRFTTQRQEPDAVRILSGVFDGLTTGTPIALQIDNVDQRSRDYSQIAAQF
                     RPGHADLTYELKYGIRDYRGGGRSSARETASRVAAGAVARRVLGAGVRIRGALVQIGP
                     HRIQRDRWDWDAVADNPFFCPDPKTATLWESYLSEVRKA"
     gene            complement(733..1713)
                     /locus_tag="VFN42_13485"
     CDS             complement(733..1713)
                     /locus_tag="VFN42_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET8997674.1"
                     /translation="MPDDQSARSAQANGRGREAERPQDVPAPGWKDIAWRAWSDVGEK
                     NLFLASGGVTYAVLLALFPGLAALVSVYGLIANPEQIQKQVGSLSGVLPAQTRQMVSS
                     ELHSLAASSSSALGIGLVISVLLALWSASRGMSGLMTAVNLAYGERETRSFFRFNAIA
                     LVLTLGTVVGGIVAVGLVGGLPAAVQAAGFGAFLNWLMLILEWPLLVVLMLAGLAVLY
                     RYAPDRDEPQWHWVTPGAITATALWIVGSILFSLYVGNFGSYNKTYGSLGGAVVLMTW
                     LYLTAFVVLLGAAINAQAERQTRKDTTAGKPLPMGERDAKAADTLGESHP"
     gene            complement(1807..3132)
                     /locus_tag="VFN42_13490"
     CDS             complement(1807..3132)
                     /locus_tag="VFN42_13490"
                     /inference="COORDINATES: protein motif:HMM:NF017835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcvB/VirJ family lysyl-phosphatidylglycerol
                     hydrolase"
                     /protein_id="HET8997675.1"
                     /translation="MKRLLLLLVLLFASLQTARAGTLNGGDYGTVHVTAPSGTMQGFV
                     VLFSHGAVWADADQAAADALAAKGAYVLGVDTEPYLHRIGAAKDCHHMQGDAEALSHQ
                     IERERGTPAYFAPIMAGIGTGGMLAEAALEEAPSNTIDGAVSIDPAATLDPRARPCAR
                     EAPGPGLPGFWDVAASPALSPEAEQTLARMQANGVKLTLHHIPAVQSEADTLVALIVP
                     HLGKPQYRPEDISDLPLIELPAAHPSDMLAVVISGDGGWRDLDKSIALKLQKWGVSVV
                     GWDSLRYFWSKKTPAQTAHDLARILRTYSARWHATHIALIGYSFGADVMPFAYNRLPA
                     DLRSKVSLMALLGFAPAADFEIRVFGWLGMPPSADALPVKDEIARVPPAIVQCFYGAD
                     EDDTYCPKLAGSGATLIRTPGSHHFGGTYDPLAQAILDRWRQRLGKPPA"
     gene            complement(3129..>4252)
                     /locus_tag="VFN42_13495"
     CDS             complement(3129..>4252)
                     /locus_tag="VFN42_13495"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HET8997676.1"
                     /translation="PYSQDLWWQFEFDDKAPRALRATLGATLFASAIAVWQMLRLAPG
                     KLVPPTPVDLLDAERIIRRQERSDAMLAMMGDKTFLFSPTRTAFLMYARHGRSWVALY
                     DPVGPREEWPVLIHQFVALAHSNGGRAAFYQVRPESLPLYLDAGLRLLKLGEEARIRL
                     PEFGLEGSRRANLRYALKRGERDGLCAEVVPPDGIAAILPELQAISEAWLRGRRAREK
                     GFSVANFDAGCLKSQSVMLVRQNGQPVAFAGFMTTDLHTDATVGAMRHTPEASPYAME
                     YLFTKLALYLKQTGYTTFSLGMAPLSGLTPAPLARPWHRVAHLVWRYGGRIYNFQGLR
                     TFKSKFGPVWEPRYLAASGDMGSFLALADIAVLAGGVGA"
BASE COUNT          721 a         1446 c         1454 g          631 t
ORIGIN      
        1 gctttgcgca cttcggacag atagctctcc cacagcgtcg ctgtcttcgg gtccgggcag
       61 aagaatggat tgtcagccac cgcatcccag tcccagcggt cccgctggat gcgatgcggc
      121 ccgatctgca ccagcgcacc gcgaatgcgc acgcccgcgc cgagcacgcg gcgggcgaca
      181 gcgccggcgg cgacacggga agcggtttcc cgggcggagg atcggccgcc gccccgataa
      241 tcgcggatgc cgtatttcag ctcgtaggtc aggtcggcgt ggccggggcg gaactgcgcg
      301 gcgatctgcg agtaatcccg cgagcgctgg tccacgttat cgatctgcag cgcgatgggt
      361 gtgccggtgg tcaggccatc gaacacgccg gagaggatcc gcaccgcgtc cggctcctgc
      421 cgctgggtgg tgaagcgcga ctggccgggt cggcggcggt cgagccaggg ctggatgtcg
      481 gcttcggtca gcgacaggcg gggcgggcag ccgtccacga tgcagccgat cgccggaccg
      541 tggctttcgc cccaggtggt gacgcggaac aggtggccga aactgttgtg gctcacgccg
      601 gtgacctgct ggttgggtgg atcatgccgg atgcaatagc acagggcggc gccagcggca
      661 ggccggcgca cgccgtactc cgccggcacg accggcgaat gccgggccgc tggggccggg
      721 gaggatgggc tgctacgggt ggctctctcc gagcgtgtcg gcggccttgg cgtcccgctc
      781 tcccatcgga agcggcttgc cggcggtggt gtccttgcgc gtctgccgtt cggcctgggc
      841 attgatggcc gccccaagca acaccacgaa ggcagtcagg tacagccacg tcatcagcac
      901 gacggcgcca cccagcgagc cataagtctt attgtagctg ccgaaatttc cgacatagag
      961 gctgaacagg atcgagccga caatccacag cgcggtggca gtgatggccc ccggggtcac
     1021 ccagtgccat tgcggctcgt cgcgatcggg ggcgtagcgg tacagcacgg ccaggccggc
     1081 cagcatcagc acgaccagca acggccattc caggatcagc atcagccagt tcaggaaggc
     1141 gccgaatccc gccgcctgca cggccgcggg caggccgccc accaaaccga ccgcgacaat
     1201 cccgccgacc accgtgccca gcgtcaggac cagcgcgatc gcgttgaacc ggaagaagct
     1261 gcgggtctcc cgttcgccgt aggcgagatt gaccgcagtc atcagcccgc tcatgccgcg
     1321 cgaagcgctc cacagcgcca gcaaaacgct gatgaccagg ccgatgccga gggcgctgga
     1381 ggaggacgcg gccaggctgt gcagttcact ggaaaccatc tgccgggtct gcgccggcag
     1441 cacgccggac agcgatccga cctgcttctg gatctgctcc ggattggcga tcaagccata
     1501 gaccgagacc agcgccgcca gaccaggaaa cagtgcaagc agcacggcgt aggtcacgcc
     1561 ccccgaggcg agaaacaggt tcttctcgcc gacatcactc caggcacgcc aggcgatatc
     1621 cttccacccg ggagctggca catcctgcgg ccgttcggct tcacggccgc gtccgttcgc
     1681 ttgcgcggat cgggcagact ggtcgtcggg catgcagggc tcgcgtcctc gtggctgacg
     1741 cggcggtaac catcggggca gcctttggtt gcgcgccggg cgccagatcg gcagccggcc
     1801 agcccgtcac gccggcggct tgcccaggcg ctgccgccag cggtccagga tcgcctgggc
     1861 cagcgggtcg taggtgccac cgaaatgatg ggagccaggc gtacggatca gcgtcgcgcc
     1921 gctgcccgcc agcttggggc aataggtatc gtcctcatcc gcgccataga agcactggac
     1981 gatggccggc ggcacgcggg cgatctcgtc cttcaccggc aaggcgtcgg cactgggcgg
     2041 catgccgagc cagccgaaga cgcggatttc gaaatccgcg gccggggcga aacccagcag
     2101 cgccatcagc gacaccttgc tgcgcaggtc ggccggcaac cggttgtagg cgaagggcat
     2161 gacatcggcg ccgaacgagt agccgatcag cgcgatgtgc gtcgcatgcc agcgcgccga
     2221 gtaggtgcgc aggatacggg caagatcgtg cgcggtctgc gccggggtct tcttgctcca
     2281 gaagtagcgc aggctgtccc agccgacgac ggacacgccc catttctgca gcttcagcgc
     2341 gatcgacttg tccagatcgc gccagccgcc gtcgccggag atcaccaccg ccagcatgtc
     2401 ggacggatgt gccgccggca gttcgatcag cggcaggtcg ctgatatcct ccggccggta
     2461 ttgcggcttg cccagatgcg gcacgatcag cgcgaccagg gtatcggcct cgctctggac
     2521 ggcaggtata tggtgcagcg tcagcttgac gccattcgcc tgcatgcgcg ccagggtctg
     2581 ctccgcctcc ggactcagcg cggggctggc ggcgacgtcc caaaaaccgg gcaggcctgg
     2641 accgggcgct tcacgcgcgc agggccgggc gcgtggatcg agcgtggccg ccggatcgat
     2701 cgatacggcg ccgtcgatgg tgttggacgg cgcctcctcc agcgcggctt cggccagcat
     2761 gccgcccgtg ccgattccgg ccatgatcgg cgcgaaatag gccggcgtgc cgcgttcgcg
     2821 ctcgatctgg tggctgagcg cctcggcgtc gccctgcatg tgatggcagt ccttggcggc
     2881 gccgatccgg tgcaggtacg gttcggtgtc caccccaagc acgtacgcgc ccttggcggc
     2941 cagcgcgtcc gcggcggcct ggtcggcgtc agcccagacg gccccgtgcg agaacagcac
     3001 cacgaagccc tgcatcgtgc cggagggcgc ggtgacgtgc acggtgccgt aatcgccgcc
     3061 gttcagcgta ccggcgcgcg cggtttgcag cgaagcgaac aacagcacca gcagaagcaa
     3121 cagccgtttc atgcgccaac gcctccggcg agaaccgcga tatccgccag cgccaggaag
     3181 gatcccatgt cgccggacgc cgccaggtag cgcggttccc ataccggacc gaacttgctt
     3241 ttgaaggtgc gcaggccctg gaagttgtag atgcggccgc cataccgcca gaccagatgc
     3301 gccacccgat gccagggccg cgccagcggc gccggtgtca acccggacag cggtgccatg
     3361 ccgaggctga acgtggtgta gccggtctgc ttcagataca gcgccagctt ggtgaacagg
     3421 tactccatcg cgtaaggcga cgcctctggt gtatggcgca tcgcaccgac cgtggcatcg
     3481 gtatgcagat cggtggtcat gaagccggcg aaagcaaccg gctggccatt ctgccgcacc
     3541 agcatcaccg attgcgattt caggcagccg gcgtcgaaat tcgccacgga gaatcccttc
     3601 tcccgtgcgc ggcggccgcg cagccacgcc tcggagatgg cctgcagctc cggcaggatc
     3661 gcagcgatac cgtccggcgg caccacttcg gcgcacagcc cgtcgcgctc accgcgcttc
     3721 agcgcatagc gcaggttggc gcggcgggag ccttccagcc cgaattccgg cagccggatg
     3781 cgcgcttcct cgccgagctt cagcaggcgc aagccggcat cgagatacag cggcaggctc
     3841 tccggccgca cctggtagaa cgcggcccga ccaccgttgc tgtgcgccag ggcgacgaat
     3901 tggtggatca gaaccggcca ttcctcgcgt ggcccgaccg ggtcgtacaa ggccacccaa
     3961 ctgcggccat gccgcgcata catcaggaac gccgtgcgcg tgggcgagaa caggaaagtc
     4021 ttgtcgccca tcatcgccag catcgcatcg ctgcgttcct gccggcggat gatgcgttcg
     4081 gcatcgagca ggtccaccgg cgtcggcggc acgagcttgc cgggggccaa gcgcagcatc
     4141 tgccagactg cgatcgccga ggcgaacagc gtggcaccga gcgtggcgcg cagggcacgc
     4201 ggcgccttgt cgtcgaactc gaactgccac cacagatcct ggctgtacgg ca
//
LOCUS       DASSZH010000626         7032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_918200, whole genome shotgun sequence.
ACCESSION   DASSZH010000626 DASSZH010000000
VERSION     DASSZH010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7032
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918200"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1156)
                     /locus_tag="VFN42_13500"
     CDS             complement(134..1156)
                     /locus_tag="VFN42_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HET8997677.1"
                     /translation="MNAPANRPTRRALIAAGGALPLVALVSRPGSAAEFHYKFATGQD
                     PTHPINTRAAEALSRIREATNGRLNIRLFPANQLGSDTDLLSQVRSGGVDFLNLSTSI
                     LATFVPVTGIVNTGFAFADYDAVWRAMDGGLGQHIRAQINKAGIVSVAGLYDNGFREI
                     TTSTRPITTPSDLAGLKLRVPPAPMLTSLFKALGAGPAPINFNEVYSALQTKVVEGQE
                     NPLAIIATTRLYEVQKFCSMTGHVWDGYCFLANRRAWAALPADVQAIAMREFDRSALD
                     ERADIARLSETLRKDLTAKGLAFNDVDRSLFRSALIHTSFYADWKAKYGATAWGLLEQ
                     TTGKLV"
     gene            complement(1194..2090)
                     /locus_tag="VFN42_13505"
     CDS             complement(1194..2090)
                     /locus_tag="VFN42_13505"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8997678.1"
                     /translation="MTDQPDRHGPVAVIGLGAMGLPMARALLRGGFAVRGYDLRQAAR
                     EAIAADGGTAADSARAAAAGAAVLMLMVVDAAQAEAALFAEGALDALATDGVVLLTAT
                     CPPAAVQALAARVRQTGRGFVDAPVSGGTAGATAATLTVMAAGTPADLAVVHPLLEAI
                     GKRIFIVGPDPGQGAVVKTVNQLLCGAHIVVAAEGLALARRLGVEGRMILDVLGGSAA
                     ASWMLNDRGPRMLDEHPPVSSAVDIFVKDLGIVLQAGRDSRAALPMAALAHQLFLAAS
                     GRGLGAEDDSQVLRCYEALNGT"
     gene            complement(2087..2983)
                     /locus_tag="VFN42_13510"
     CDS             complement(2087..2983)
                     /locus_tag="VFN42_13510"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET8997679.1"
                     /translation="MPPKLFASGVGAPEGPISLPDGSMYVTEMSADTSCVTRLDPQGR
                     RHVIKKTGGRPNGLALDGDGCIWIAEAILRCVICIDPDGTEIARIEGDGEGRFLFPND
                     LAFGPDGHLYMTDSGMVASDFISGQNFVENFMELPWDGRVYEIDPKAGKVVRKIDRGI
                     RFTNGIAFDGAHHLHANASFTGEVYRYDVFGAGAPRRELFGNVLRPDDRPVFKGPDGM
                     KFGADGRLYCTVYNQRNVTVLDQDGKVADRLMLDGEQPTNLAFSLDGTNLLVTEVSKG
                     QVEAIPAPCAGLALHYPKVGLR"
     gene            complement(3063..4052)
                     /locus_tag="VFN42_13515"
     CDS             complement(3063..4052)
                     /locus_tag="VFN42_13515"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET8997680.1"
                     /translation="MSRKGCRLFAAAVAAVLTCGVAAGRTEAKDKYTVVYLAPSLDIS
                     YWQWVAYGVKTQAQKLGMDYIEMTSENSPSTQMNNARTAVTRGVNAVVIGPVSSTSTP
                     PLLRFLKEHKIPVAFTGIGPAPGQTDYTSSVTANNYDTGVAEGKFICDQAKERGGNKV
                     GMLSLPQDRENAQKYLKGAQESFKAGGCDLVQILQTHGLTVNEAVQQANDLLTAHPDI
                     KAIYGMYDEAATGAAKALETRGLTGKIAIATADGSPTTIRLLRDGKIQGLFLQEAVGQ
                     GIDATQQVYNALSGKKVTKELPLPEPLVTKDTIDSPETQAKIKRVYPPSAGAY"
     gene            complement(4084..5097)
                     /locus_tag="VFN42_13520"
     CDS             complement(4084..5097)
                     /locus_tag="VFN42_13520"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8997681.1"
                     /translation="MKAAERPADAAASASDITAGQRASGARLLATYRTEAAIAVAIVL
                     IEVVVGLLVPQALTWGNFADVTQAAAPLVIMAVGVLLVVITGGIDLSVGSVFSLTGMV
                     TALAMAHGLGGITSTACGLGVGLIVGLFNGALVTFAGLAPFVVTLITYAAAGSLAFIV
                     TDGHSMPVPDANYYLLNSGTLVPGVPNFALFCIVLTVVVEFALRKIVAGRWLYAIGSS
                     SEASRLLGIPVRATRLATYAICSLLASFAAVLSLSYISNAEATAGSSLMLQAIAAVVI
                     GGASLSGGTGSAIGAVLGALMITVIQNGVNLIGINSFWQGSVTGMVILVAVLIDRVGK
                     ARR"
     gene            complement(5094..6587)
                     /locus_tag="VFN42_13525"
     CDS             complement(5094..6587)
                     /locus_tag="VFN42_13525"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET8997682.1"
                     /translation="MDTPGYLLEAREVQKSYGATKVLRGVNFAVAASEIHALLGGNGA
                     GKSTLLKIVTGNVSRDGGEVLYRGVDVDSSEGAAARARGVAVVHQETALLPDLTVAEN
                     IHLPHLARGAGLFSLREAERVAEAALAQIDAAFAQRALRRRVAELTLHERQVVEIARA
                     LSAGAQLLLLDEPTANLTAAETERLFALLRRLTGQHGISVVFVSHRMREIREIASVCT
                     ILRDGVSAISRTPLTALSDADIVGTMGQATAVEAVVRSQTVPAAVTPGEAFRAERGNT
                     RLDIPPGRIIGLAGAPAGPSALIDALTGARTDSGWRITRGGRPLPLRSPRQAVRQGIG
                     FVSGDRAGKGVLATLPIVDNMLAAARIRQRRIFVGNSETLEGEGLLRTLRIKAASLWA
                     LPATLSGGTQQKLLIARWLNVAPSLLVLEEPTRGVDIGTKRDLYALVRQMAERGVGVV
                     WWSTEYMELVELCHAVLAFDPDGTPSGMLTGRELDETHLAHATGMAA"
     gene            complement(6593..>7032)
                     /locus_tag="VFN42_13530"
     CDS             complement(6593..>7032)
                     /locus_tag="VFN42_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET8997683.1"
                     /translation="VPLVLDEYDYRFELGRAKLLRDGSDALIISSGIMTMRALEVAER
                     LRTDRIGVAVLHTPTIKPLDEQAILDQAARPGRLVVVAENHTVIGGLGEAVAALLLRA
                     RVTPPFRQIGLPDAFLDAGALPTLHDRYGISAETMAAQLRSWL"
BASE COUNT         1141 a         2469 c         2261 g         1161 t
ORIGIN      
        1 gtttccacca gcacgagcac gccggcaggg atctcgacga tcaggcccag cgccgtgtcc
       61 gccgctgtga cccagccagg accgccgccc cgcggctgct gtgcagtacg cgaagtgtca
      121 agcagcatcg tgttcagacc agcttgccgg tcgtctgttc cagcaggccc caggcggtcg
      181 caccgtattt tgccttccag tccgcgtaga aacttgtgtg gatgagggcg gagcggaaca
      241 ggctcctgtc cacatcattg aacgcgagac cttttgccgt cagatccttg cgcaacgtct
      301 cgctgagccg cgcgatgtcg gcgcgctcgt cgagggccga gcggtcaaac tcccgcatcg
      361 cgatcgcctg tacatccgcc ggcagtgccg cccaggcgcg tcggttggcc aggaagcagt
      421 agccgtccca tacatggccg gtcatgctgc agaatttctg cacttcgtag agccgcgtcg
      481 tggcgatgat ggcgagcggg ttttcctgcc cctcgaccac cttggtctgc agcgcggaat
      541 agacctcgtt gaaattgatc ggcgcaggcc cggcaccgag tgccttgaac aacgaggtca
      601 gcatcggcgc gggaggcacg cgcagtttca gccctgcgag atcggacggc gtcgtgatcg
      661 gcctggtcga ggtcgtgatc tcgcgaaagc cgttgtcgta gagtccggcc accgagacga
      721 taccggcctt gttgatctgt gcgcggatat gctggccgag tcccccgtcc atcgcgcgcc
      781 agaccgcatc gtagtccgca aacgcgaagc cggtgttcac gatgcctgtc accggcacga
      841 aggtggccag gatcgatgtc gagagattca ggaaatcgac acccccgctg cgcacctgcg
      901 acaacaggtc cgtgtcggag ccgagctggt tggcgggaaa aagccggatg ttcagccggc
      961 cattcgtcgc ctcccggata cgcgacaatg cctcggccgc ccgcgtgttg atcggatggg
     1021 tcggatcctg cccggtcgcg aacttgtagt ggaattcagc ggcagacccc gggcggctga
     1081 ccaatgccac cagcggcagg gcgcccccgg cggcgatcag ggcacggcgg gtcggtcggt
     1141 tcgcaggagc gttcatgatc gtttcctctg ggattgtcgg gaacctgggc ggatcaggtc
     1201 ccgttgagcg cctcgtagca acgcagcacc tggctgtcgt cctcggcccc gagcccgcga
     1261 ccggacgcag cgaggaaaag ctgatgcgcc agcgccgcca tcggcagcgc cgccctgctg
     1321 tcgcgcccgg cctgcaggac gatgccaaga tccttcacga agatgtccac cgcgctggaa
     1381 accggcggat gctcgtccag catgcgcggg ccgcggtcgt tcagcatcca gcttgccgcc
     1441 gccgagccgc ccagcacgtc gaggatcatc cgtccctcca cgcccagcct gcgcgccagc
     1501 gccagcccct ccgccgcgac cacgatatgg gcgccgcaca gaagctggtt gaccgtcttg
     1561 accactgcgc cctgaccggg gtcgggaccg acgatgaaga tacgcttccc gatcgcctcc
     1621 agcagtgggt ggaccacagc gagatcggcg ggcgtccccg ccgccatcac ggtcagcgtc
     1681 gccgccgtcg cccccgcagt gccgccggac acgggcgcat cgacgaaacc tcggccggtc
     1741 tgtcgcacgc gggcggccag tgcctgtacg gcggcgggcg gacaggtcgc ggtcagcagc
     1801 acgaccccat cggtcgccag cgcatccagc gcaccttcgg caaacagcgc cgcctccgcc
     1861 tgcgccgcat cgacgaccat cagcatcagc actgcagcgc ccgccgccgc tgcgcgggcg
     1921 gagtcggccg ccgtgccgcc atccgctgca attgcctccc gcgcggcttg ccgcagatca
     1981 tagccgcgca ccgcgaagcc gccccgcagc agcgcccgtg ccatcggcag acccattgcg
     2041 cccaggccga tgacggcgac gggaccgtgc ctgtccggct gatccgtcac cggagcccga
     2101 ccttcggata gtgcaacgcc agccctgcgc agggcgccgg aatggcttcg acctggccct
     2161 tggaaacctc ggtcacaagc aggttcgtcc catcgagcga gaaggcgaga ttggttggct
     2221 gctccccgtc cagcatcagc cggtccgcca ccttgccgtc ctggtcgagc acggtgacgt
     2281 tgcgttgatt atagacggtg caataaagcc gcccatccgc cccgaacttc atgccatccg
     2341 gccccttgaa gacgggccgg tcgtccggcc gcagcacgtt tccgaacagc tcgcgtcttg
     2401 gcgcgccggc accgaatacg tcgtagcgat agacctcgcc cgtgaaggac gcgttggcat
     2461 ggagatggtg ggcgccgtcg aacgcgattc cgttggtgaa gcggatgccg cggtcgatct
     2521 tgcgaacgac cttcccggcc ttggggtcga tctcgtaaac ccgcccatcc cagggcaact
     2581 ccatgaaatt ctcgacgaaa ttctggccac tgatgaagtc gctggcgacc atcccggaat
     2641 cggtcatgta gagatggccg tccggtccga acgccaggtc gttggggaac aggaagcgcc
     2701 cttcgccatc gccctcgatg cgcgcgatct cggtcccgtc cggatcgatg cagatcacgc
     2761 agcggaggat cgcctcggca atccagatgc aaccgtcgcc gtcgagggcg agtccgttgg
     2821 gccgcccgcc ggtcttcttg atgacatgcc gacgaccctg cggatcgagg cgggtgacgc
     2881 agctcgtatc agccgacatc tccgtcacat acatcgatcc atccggcagg ctgatcggcc
     2941 cttccggcgc gccgacgccc gaagcaaaca gcttgggtgg catcctctcc tcccgttctg
     3001 gttgatgtgg ctttccggaa cggccagcgg cggccgatgg aaaccgccgc tggccgcgtc
     3061 gctcagtacg ccccggcgga cggcggatag acgcgcttga tcttcgcctg ggtttccgga
     3121 ctgtcgatcg tgtccttcgt gaccagcggc tcgggcagcg gcagttcctt cgtcactttc
     3181 ttgccgctga gagcgttgta cacctgctgc gtcgcatcga tgccctggcc gacggcttcc
     3241 tgcaggaaca gcccctggat cttcccgtcg cgcagcagcc tgatcgtggt ggggctgccg
     3301 tccgcggtcg cgatcgcgat cttgccggtc aggccgcgcg tctccagcgc cttggccgcg
     3361 cccgtggcgg cttcgtcata catgccgtag atcgccttga tgtccggatg ggcggtcagc
     3421 aggtcattgg cctgctgcac agcctcgttc accgtcagcc cgtgcgtctg caggatctgc
     3481 accaggtcgc agccgcccgc cttgaacgac tcctgcgcgc ccttgaggta tttctgcgca
     3541 ttctcgcggt cctgcggcaa cgacagcatg ccgaccttgt tgccgccgcg ctctttggcc
     3601 tggtcgcaga tgaacttccc ctctgcgacg ccggtgtcgt agttgttggc cgtgacggaa
     3661 gaggtgtagt cggtctgccc gggcgccggg ccgatgccgg tgaaggccac cgggatcttg
     3721 tgctccttca ggaagcgcag cagcggcggt gtgctcgtcg agctgaccgg accgatcacc
     3781 acggcgttca caccccgcgt caccgccgtg cgcgcattgt tcatctgcgt gctgggcgag
     3841 ttctccgacg tcatctcgat gtagtccatg ccgagctttt gcgcctgggt cttcacccca
     3901 taggcaaccc actgccagta ggagatgtcc agcgacggtg cgagatagac gacggtgtat
     3961 ttgtccttcg cctccgtccg gccggcggcc acgccgcatg tgagcaccgc ggcaactgcg
     4021 gcagcgaaaa gacggcaacc cttgcgtgac atggtggttt ccctccgtga ttgttggtgt
     4081 gtgttagcgg cgcgccttgc cgacgcggtc gatcagcacc gcaaccagga tcaccatccc
     4141 ggtcacggac ccttgccaga agctgttgat cccgatcagg ttcacgccgt tctggattac
     4201 cgtgatcatc agcgcgccga gcaccgcgcc aatggccgag ccggtgccgc ccgacaggct
     4261 ggcgccgccg atcaccaccg ctgcaatcgc ctgcaacatc aggctcgatc ccgctgtcgc
     4321 ctcggcgttg ctgatgtagg acaggctgag cacggcggcg aaggacgcca acaggctgca
     4381 gatcgcgtag gtggccaggc gcgtggcgcg cacgggaatg ccgagcaggc gggacgcttc
     4441 gctgctgctg cctatggcat agagccagcg ccctgccacg attttgcgca gcgcgaactc
     4501 caccacgacc gtcagcacga tgcagaacag cgcgaagttc ggcacgcccg gcaccagcgt
     4561 gccggaattc agcaggtagt aattggcgtc cggcaccggc atcgaatgtc catcggtgac
     4621 gatgaaggcc aggctgccgg cggcggcata cgtgatcagc gtcacgacaa agggggcgag
     4681 gccggcaaat gtgaccagcg cgccattgaa caggccgacg atcaatccca cgcccaggcc
     4741 gcatgccgtg ctggtgatgc cgcccagccc gtgcgccatc gccagcgccg tgaccatgcc
     4801 ggtgagcgaa aagaccgatc cgacggagag atcgatgccg ccggtgatca ccaccagcag
     4861 cacacccacc gccatgatga cgagcggcgc cgcggcctgg gttacgtcgg cgaagttgcc
     4921 ccatgtcagt gcctggggta ccagcaggcc gaccaccacc tcgatcagca cgatggcgac
     4981 cgcgatcgcc gcttcggtcc ggtaggtcgc gagcaggcgc gctcccgatg cgcgttgacc
     5041 cgccgtgatg tcgctggccg aagccgccgc gtcggccgga cgctccgctg ccttcatgcc
     5101 gccatccccg tcgcatgggc caggtgcgtc tcgtccaact cccggccggt gagcatcccg
     5161 gacggggtcc cgtccgggtc gaaggcgagg accgcatggc acagttccac cagttccata
     5221 tactcggtgg accaccacac gacaccgacg ccacgttcgg ccatctgccg caccagggcg
     5281 tacagatcgc gcttggtgcc gatatcgacg ccgcgcgtcg gctcctccag cacgagcagg
     5341 cttggcgcga cattcagcca gcgtgcgatc agcaacttct gctgcgtgcc gcccgagagc
     5401 gtcgccggca gtgcccaaag agaagcggcc ttgatgcgca gcgtgcgcag caggccctca
     5461 ccttccagcg tctcactgtt gccgacgaag atgcgccgct ggcgaatgcg cgccgcggcc
     5521 agcatgttgt ccacgatcgg cagggtcgcc agcacgccct tgccggcacg gtcgccggag
     5581 acaaagccga tgccctgccg caccgcctgc cgcgggctgc gcaagggaag cggacggccg
     5641 ccgcgggtga tgcgccagcc gctgtcggtc cgcgcgccgg tcagggcgtc gatcagcgcc
     5701 gacggaccgg ccggcgcgcc cgccagcccg atgatgcgtc cgggcgggat gtccagccgc
     5761 gtgttgccgc gctccgccct gaacgcctcg cccggtgtga cggcggccgg cacggtttgc
     5821 gaccgcacca ccgcctccac tgccgttgcc tgtcccatcg tcccgacgat gtccgcatca
     5881 ctgagagcgg tgagcggcgt gcggctgatc gcgctgaccc catcgcggag gatggtgcag
     5941 accgaggcga tctcccggat ttcccgcatc cggtgcgaga cgaacaccac cgagatgcca
     6001 tgctggccgg tcagccggcg caacagcgcg aacagccgtt cggtctccgc ggccgtcaga
     6061 ttggcggtcg gctcgtcgag cagcagcaac tgtgccccgg cgctgagtgc gcgggcgatt
     6121 tccacgacct gccgctcatg cagcgtcagc tcggcgacgc gtcggcgcag ggcgcgttgc
     6181 gcaaaggctg cgtcgatctg tgcgagggcc gcctcggcca cacgctccgc ctcgcgaaga
     6241 ctgaacagcc cggcgccgcg cgccagatgt ggcagatgga tgttctccgc caccgtcaga
     6301 tccggcagca gcgccgtctc ctggtgcacg accgcgacgc cgcgcgcccg cgccgcggcc
     6361 ccctccgagg agtcgacatc caccccccga tagagaacct cgccgccgtc gcgggacacg
     6421 ttgccggtca cgattttcaa cagcgttgac ttgcccgcgc cgttgccgcc cagcaacgcg
     6481 tggatttccg acgccgccac ggcgaagttc acgccgcgca gcactttcgt ggcgccgtag
     6541 ctcttctgta cctcgcgcgc ttcgagcagg tatccgggcg tatccatgac gtctacagcc
     6601 acgacctgag ttgcgccgcc atcgtctccg ccgagatgcc gtagcggtca tggagcgtcg
     6661 gcagcgcgcc cgcgtcgagg aaggcatcgg gcaggccaat ctgtcggaac ggaggcgtca
     6721 cccgcgcgcg cagcagcagc gccgcgaccg cctctcccag gccgccgatc acggtatggt
     6781 tttcggcgac gacgaccagc cggccgggac gtgccgcctg atcaaggatt gcctgttcat
     6841 ccagcggctt gatcgtgggc gtatgcagta ccgcgacgcc aatgcggtcg gtgcgcagcc
     6901 gttccgccac ttcaagcgcc cgcatcgtca tgatgccgga ggagatgatc agtgcgtccg
     6961 agccgtcacg cagcagcttc gcccggccca gctcgaagcg atagtcgtac tcgtccagca
     7021 ccagcggcac ct
//
LOCUS       DASSZH010000627         3868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_236561, whole genome shotgun sequence.
ACCESSION   DASSZH010000627 DASSZH010000000
VERSION     DASSZH010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236561"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="VFN42_13535"
     CDS             complement(<1..1212)
                     /locus_tag="VFN42_13535"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8997684.1"
                     /translation="MPAEHAVVELTQLRHTFGGNIALHDISTTIQPGMITGLVGPDAS
                     GKTTLLRLIAGLLHPAAGGVRVLGFDMARESAAVHPFIGYMPQRFGLYEDLSVAENLD
                     LFADLHTMPLALRTERIARLLRFTGLAPFTARLAGKLSGGMKQKLGLACALLARPRLL
                     LLDEPSVGVDPASRRELWAIVNAMLEKGRTEEMAVVWATAYLDEAERCGRVLLLHQGR
                     LLSDAPPAEFLSPLHGRVFRMTVPAGQRRAIARYAAAHAAVLDAQVTGDALRIVLRAG
                     AAVPAAPALGGTALDPVAPRFEDGFIARLAETPAAESGNGQQTPPPPLVGGGRGEGSC
                     ELPPCSSGTFDPSPHPPPARGGGGVSAPASGTSEPPIIVHDLVRRFGAFTAVDHISFS
                     VQRGEIFGLLGP"
     gene            complement(1202..2200)
                     /locus_tag="VFN42_13540"
     CDS             complement(1202..2200)
                     /locus_tag="VFN42_13540"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET8997685.1"
                     /translation="MKRALLGMILLAGVAGLGWLAAQRLAATPDPNVLYGNVEIRQVD
                     LSFETEGKIIAMPEHEGDRLHRGEVIAELDPATLQSAVALAAARRDAAKASLDVLLAG
                     TRPEEIAQARANYAAAQASLANAQASFARQKDLAARSVSSQQQLDDARMALDSAVARV
                     AQTKAAMEEAVNGPRAEDIAAARANLRAAQATLDLANTQLDHTRLIAPVDGTVMTRVI
                     EPGTVVLPSAAVYSVAIDDETWVRAFAPEPLLPRLAPGTRVTLTTDGGTSYEGRIGYV
                     SPAAEFTPKTVETPELRTQLVYRFRVRVQHPDTGLRQGMPVTIHLPRTSPEAEHAG"
     gene            complement(2278..3354)
                     /locus_tag="VFN42_13545"
     CDS             complement(2278..3354)
                     /locus_tag="VFN42_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8997686.1"
                     /translation="MSQPLTQFGRIFPPRTDWLAKAPAEPILDPDLPIIDTHHHLWHR
                     QSSGARGESWEVPSNRYLLEEFLADCATGHNIVASVFLQCHSMYRADGPQEMRPVGET
                     EFVAGVAAMSDSGGYGETRVAAGIVGYADLTKGDWVQPVLEAHIRAGGGRFRGVRHSA
                     GYDSDPVIGNSAPDMQPGLYLRPDFRAGLRRLSALGLSLDAWAFHPQLPDVVDLARAF
                     PGTNIIVGHCGGPLGYGRYAGKRDEVFADWKASVTELAKCENVTMKLGGMMMRLAAYD
                     YMALPAPPSSQQLADHWRPYVETCLELFGPHRCMAESNFPVEKMGIGYAALFNALKRL
                     CAGGSAEDKQAIFAGTARRVYRLD"
     gene            complement(3423..>3868)
                     /locus_tag="VFN42_13550"
     CDS             complement(3423..>3868)
                     /locus_tag="VFN42_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8997687.1"
                     /translation="LFDTLGRRVMITATYALSGVLMALTGWLFALGVLNAVEQTAAWT
                     VIFFVASAAASSAYLTVGESFPLEVRAVAIALFYAFGTGVGGVAGPALFGWLIDSGSR
                     VSIMWGYLFGGALMLIGAVVAARLGVRAERRSLEDVAPPLSAVER"
BASE COUNT          640 a         1344 c         1295 g          589 t
ORIGIN      
        1 ggggccaagc agaccgaaga tctcaccgcg ctgcacggaa aaactgatgt ggtccaccgc
       61 ggtgaacgcc ccgaaccggc gcaccagatc gtgcacgatg atcggcggtt cactggtccc
      121 ggaggctggt gctgagacac ctccccctcc ccttgcggga gggggatggg gggaggggtc
      181 gaacgttcca cttgagcatg ggggcagttc gcacgacccc tccccccttc cccctcccac
      241 aaggggcggg ggaggagtct gctgcccgtt accggactct gctgcgggcg tctcggcaag
      301 ccgtgcgatg aagccgtcct cgaagcgtgg cgccaccgga tccagcgccg ttccacccag
      361 cgccggtgca gcgggaaccg ccgccccggc ccgcagcacg atgcgcagcg cgtctcctgt
      421 cacctgcgca tccagcaccg ccgcgtgcgc cgccgcgtaa cgggcgatgg cgcggcgctg
      481 gccggccggc accgtcattc ggaacacgcg gccgtgcagc ggactcagga actccgcggg
      541 cggggcatcg ctcagcagcc ggccctggtg cagcagcagc acccggccgc agcgttccgc
      601 ctcgtcgaga taggccgtgg cccagaccac cgccatttct tcggtgcgtc ctttctctag
      661 catggcgttc acgatcgccc acagctcacg ccgcgatgcc ggatcgacgc caaccgaggg
      721 ctcgtccagc agcagcaggc gcgggcgtgc cagcagggcg caggccaggc cgagtttctg
      781 cttcatcccg cccgacagtt tgccggccag ccgcgcggtg aagggcgcca gcccggtgaa
      841 ccgcagcagc cgcgcaatgc gttccgtgcg caatgccagc ggcatggtgt gcagatcggc
      901 gaacaggtcg aggttttccg cgacactcag gtcttcatac aggccgaagc gctgcggcat
      961 gtagccgatg aacggatgca ccgcggcaga ctcgcgtgcc atgtcgaagc cgaggacgcg
     1021 cacgccgccg gccgccggat gcagcaggcc ggcgatcagg cgcagcaggg tggtcttgcc
     1081 ggacgcgtcg gggccgacca ggccggtgat catgcccggc tggatggtgg tggaaatatc
     1141 gtgcagggcg atgttgccgc cgaaggtatg acgaagctgc gtcagctcga ccacggcgtg
     1201 ctcagccggc atgctcggct tccggcgatg tccgcggcag gtggatggtc accggcatgc
     1261 cctgccgcaa tccggtatcc ggatgttgta cccgcacccg gaagcgatag accagttgcg
     1321 tgcgcagttc cggcgtctcc accgtcttcg gggtaaattc cgccgcgggc gaaacgtagc
     1381 cgatacggcc ctcgtacgaa gtgccgccat ccgtggtcag cgtgacccgc gtgcccgggg
     1441 caagccgcgg cagcagcggc tcaggcgcga aagcccgcac ccaggtctcg tcgtcgatcg
     1501 ccaccgagta caccgcggca ctcggcaaca cgacggtgcc tggctcgatc acccgcgtca
     1561 tcaccgtgcc gtccaccgga gcgatcagcc tggtatggtc aagttgggtg tttgccaggt
     1621 ccagcgtcgc ctgcgctgcg cgcaggttgg cacgcgcggc ggcgatgtcc tcggcgcgcg
     1681 gaccgttcac cgcctcctcc atcgcggcct tggtctgcgc cacgcgcgcg acagcggaat
     1741 ccaatgccat gcgcgcatcg tcgagttgct gctgcgagga caccgatcgt gcggcgagat
     1801 ccttttgcct ggcgaagctg gcctgtgcat tcgccagcga ggcctgcgcg gcggcatagt
     1861 tggcgcgggc ctgcgcaatt tcctccggcc gcgtgccggc caacaacacg tccagcgacg
     1921 ccttcgctgc atcgcgccgc gctgccgcca gcgccactgc gctctggagg gtggccggat
     1981 cgagttcggc gatgacttcg ccccggtgca ggcggtcgcc ttcgtgttcc ggcatggcga
     2041 tgatcttgcc ttcggtttcg aaggaaagat ccacctggcg gatttccaca ttgccgtaca
     2101 gcacgttggg atcgggggtg gctgccagcc gctgcgcggc cagccagccc aggcctgcca
     2161 caccggcaag caggatcatt cccaatagcg cacgcttcat ccaaccggtc cgtagtgccg
     2221 gggccagcct agcggcgccg ggggcggtcg ccttgattca ggtcaacagc gctcgggtca
     2281 gtccagccga tacacgcggc gtgcggttcc ggcgaagatg gcctgcttgt cttccgctga
     2341 tcctccggca cacaggcgct tcagcgcgtt gaacagcgcg gcgtagccga tgcccatttt
     2401 ctccaccggg aagttgctct ccgccatgca gcggtgcggc ccgaacaact ccaggcaggt
     2461 ctcgacatat ggccgccagt ggtccgcaag ctgttgggaa gatggcggcg ccggcagcgc
     2521 catatagtca tacgctgcca gccgcatcat catgccgccg agcttcatcg tcacgttctc
     2581 gcactttgcg agttccgtca cggatgcttt ccaatcggcg aacacctcgt cacgcttgcc
     2641 ggcgtagcgg ccataaccca gcgggccgcc gcaatggccg acgatgatgt tggtgccggg
     2701 aaatgcccgc gccagatcga cgacatcggg cagttgcgga tggaaggccc aggcatccag
     2761 cgacaggccc agtgcggaca ggcgccgcag cccggcgcgg aaatcggggc gcaggtacag
     2821 gcccggttgc atatccgggg cgctgttgcc gatgaccgga tcggaatcgt agccggcgga
     2881 atggcgcacg ccgcgaaacc ggccgccgcc ggcacggata tgcgcctcca gcacgggctg
     2941 cacccaatcg cccttggtca ggtcggcata gccgacgatc cccgccgcaa cacgcgtctc
     3001 gccatagccg ccactgtcgc tcatcgccgc cacacccgcg acgaactcgg tctcgccgac
     3061 cggccgcatc tcctgcgggc cgtcggcgcg atacatggaa tggcactgca ggaacaccga
     3121 ggcgacgatg ttgtggccgg tggcgcagtc cgccaggaat tcctccagca gatagcggtt
     3181 gctgggcacc tcccatgact caccgcgcgc gccgctgctc tggcgatgcc acagatggtg
     3241 atgcgtatcg atgatcggca ggtcgggatc gaggatgggt tccgccggcg ccttggcgag
     3301 ccaatcggtg cgcggcggga agatgcggcc gaactgggtg aggggctggc tcatggcgtt
     3361 tcctccgttg cgggcattgg gtgcattaaa gccgatcgca tcgggcgcgg taaccgcccg
     3421 ggtcagcgct ccacggccga caagggcggc gccacgtcct ccagcgaccg gcgttcggcc
     3481 cgcacgccca gccgagccgc caccacggcg ccgatcagca tcagcgcgcc gccgaacaga
     3541 tagccccaca tgatcgatac gcgcgagccg ctgtcgatca gccagccgaa cagcgccggg
     3601 cccgccacgc cgccgacgcc ggtgccgaac gcgtagaaca gtgcaatggc cacggcgcgc
     3661 acttccagcg ggaagctctc gcccaccgtc agataggccg agcttgcggc ggcggaggcg
     3721 acgaagaaga tcacggtcca ggcagcagtc tgttccacgg cgttcagcac gccaagcgcg
     3781 aacagccagc cggtgagggc catcagcacg ccggacaagg cgtaggtcgc cgtgatcatc
     3841 acccgccgcc cgagcgtgtc gaacaggc
//
LOCUS       DASSZH010000628         5488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1651666, whole genome shotgun sequence.
ACCESSION   DASSZH010000628 DASSZH010000000
VERSION     DASSZH010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1651666"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFN42_13555"
     CDS             complement(<1..230)
                     /locus_tag="VFN42_13555"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8997688.1"
                     /translation="MPDVLIAGAGLCGLTTALALLQRGHRVRLFEQASELREVGAGVQ
                     LGANGTRLLLALGLGDAMRRIVCVPEGKEMRL"
     gene            complement(223..1272)
                     /gene="gtdA"
                     /locus_tag="VFN42_13560"
     CDS             complement(223..1272)
                     /gene="gtdA"
                     /locus_tag="VFN42_13560"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HET8997689.1"
                     /translation="MNDSAHPTGPSDRQAFYDRIAPHNLAPLWERLHALVTRSPVTPV
                     QPAIWHYDSVVRPHLMRAASLITAKEAERRVLILENPGLRDQTCITHSLFAGLQLIMP
                     GEVAPAHRHAQSALRFIIEGRGAYTAVEGERTLMQPGDFVLTPSWTWHDHGNDTDQPM
                     VWLDGLDIPIVRLLDASFAEPANADSQAVSRPVGDSYARYGANMLPVDYKPGRASPVF
                     SYPYERSREALATLARNGAPDACHGHKLRYVNPATGGSPMPTIGACMQLLPQGFSGAP
                     YRSTDGTVFSVVEGEGETVIGDTVFHWKPRDIFVVPPWAWHQHRAATEAVLFSFSDYP
                     VQHVLGLWREERGHA"
     gene            1816..2334
                     /locus_tag="VFN42_13565"
     CDS             1816..2334
                     /locus_tag="VFN42_13565"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HET8997690.1"
                     /translation="MLLALVGLLAPILQAHAQASLESAIEATFLLKFPPFITWHQGLP
                     PDSFTVCVLGDSPVTGLLRQAAAGLSVDQHPVRVRHVSAATASSGCQELYLAGSDPTA
                     IATALAATRGTPVLTVTDGQTDPTAKGILNFVLTDGHVRFEVDLNAAAANGISISSKL
                     LSIAVHVNGSAH"
     gene            2348..4123
                     /locus_tag="VFN42_13570"
     CDS             2348..4123
                     /locus_tag="VFN42_13570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8997691.1"
                     /translation="MSRLWRVTPAIAAATVVVLLLAAVFLGHYIDRANLAQKVTEATE
                     QAKILASTASAAVAFSDRQAAGEYLGAIRANPEVEWAAIYDAKGQLFASFSRSDGRPP
                     DAAPPLRPPFVESGHINVLVPIVQKGNRLGTVYLHTPTEPVERRLERYAVIGLFAVMA
                     ALIVAVLGIAQGALTRANTELARRAGELALANTSLREQIEQREKVEEALRQAQKMEAI
                     GQLTGGVAHDFNNLLQVILGNLERLRRRALDPMEAHRLIDAALRGAERAATLTQRLLA
                     FSRRQPLAPKVLEVNKLVTGMSDLLSRTLGENIRIETVLAGGLWRVSADENQLESALL
                     NLAVNARDAMQGAGKLTIETGNAYLDDAYVRNEEEVKPGQYVLIAVSDTGSGMSKDIL
                     DRVFEPFFTTKEIGQGTGLGLSMVYGFVKQSGGHVKLYSELGQGTTVKLYLPRLLTEP
                     AQAQAPQSAASLPRGHRNELVLVVEDEDDVRALTVDTLQELGYDVLQAMDGASALRLL
                     ESEPGIRLLFTDVGLPGGLNGRQLADAARQTRPGLKVLFTTGYARNAIVHHGRLDPGV
                     QLISKPFSDTDLAHKVRQMLDGTEV"
     gene            4201..>5488
                     /locus_tag="VFN42_13575"
     CDS             4201..>5488
                     /locus_tag="VFN42_13575"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET8997692.1"
                     /translation="MTDSTWRDRAGTRFQCEKYQATSRRGMVVTNHPLASAAGVEMLA
                     AGGNAIDAAIAAFFTLTVVEPMMVGVLGGGMAHIRLSDGNHVVLDGQSMAPLATGPTT
                     YKPDPQAAPGTMDAIGRKNALGPSAVATPGNLMGWCQALERHGRFSLADVMEPAIRHA
                     SRGFRVTPYLNECVTDCAADLALDREIAALYLPNGAPIQPGARLVTGDYAETLRSIAR
                     EGPNLLYGGALGRLYTDHMARNGGYLGMDDLTRYRTIDRQALRGTYRGHEIVGPPPPS
                     SGPLHIIQMLNILEGYDIRALGFGSADTLHLLAEVLKIAFADRTAATADPAFVPVPVA
                     KLLSKDYAEQRRSRIDLQRAQQWSAEVAPGEGAHTTHLTVADGDGNVVASTQTINSLF
                     GARYIVPGTGMIPNNYMFVFTPHPGQANSVAPGKRIT"
BASE COUNT          933 a         1859 c         1847 g          849 t
ORIGIN      
        1 cacagccgca tctccttgcc ttccggcacg cagacgatgc ggcgcatcgc gtcgccgagc
       61 ccgagcgcga gcagcaggcg ggtgccgttg gcgccgagct gcacgccggc accgacttcg
      121 cgcagctcac tggcctgttc gaacaggcga acgcggtggc cgcgttgcag cagcgccagc
      181 gcggtggtca ggccgcacag gccggcgccg gcgatcagga cgtcaggcat gcccgcgttc
      241 ttcgcgccac aggcccagca cgtgctgcac cggataatcg gagaagctga acagcacggc
      301 ctcggtcgcg gcgcggtgct gatgccaggc ccagggcggc acgacgaaga tatcgcgcgg
      361 cttccagtgg aagaccgtgt cgccgatcac cgtttccccc tcgccttcca ccacggagaa
      421 cacggtgcca tcggtgctgc ggtacggggc gccggagaag ccctgcggca acagttgcat
      481 gcaggcgccg atcgtcggca tcggcgagcc accggtcgcc ggattcacat agcgcagctt
      541 gtggccgtgg caggcatccg gtgcgccgtt gcgcgccagc gtcgccagcg cctcacgcga
      601 gcgttcatac ggatagctga acaccggtga cgcccggcct ggcttgtagt ccaccggcag
      661 catgttggcg ccgtagcggg cgtaggaatc gccgaccggg cgcgacacgg cctgactgtc
      721 cgcgtttgcc ggctcggcga aactggcatc cagcagacgc acgatcggga tgtccagccc
      781 gtccagccag accatcggct gatccgtgtc gttgccatgg tcgtgccagg tccagcttgg
      841 ggtcagcacg aaatcacctg gctgcatcag ggttcgttca ccctccaccg cggtgtaggc
      901 gccacgccct tcgatgatga agcgcagggc ggattgcgca tggcggtgtg ccggcgcgac
      961 ctcgcccggc atgatcaact gcagcccggc gaacagcgaa tgggtgatgc aggtctggtc
     1021 gcgcaatcca gggttttcca ggatcagcac gcggcgttcg gcttccttgg cggtgatcag
     1081 gctggcggcg cgcatcaggt gcggacgcac cacggaatca tagtgccaga ttgccggttg
     1141 gaccggcgtg accgggctgc gcgtcaccag cgcgtgcaac ctttcccaga gcggggccag
     1201 attgtgcggc gcaatgcgat cgtagaacgc ctgccgatcg gatgggccgg tgggatgcgc
     1261 cgagtcgttc ataggacgcc gcctttctca ttgggcctgc cccggatgta gcgcagtttg
     1321 gcaatggccg gaaccgcgcc gggcgctgcg ttgcctgtgc tgtacggttc cccgccgtca
     1381 cgttgatttg atcgagcctc cgcccgcacc gccacgaggg gctcgaatcg caacttgcta
     1441 taccgcgcgt gaggtcatca cgatctgcac ctggggtggc ggtatggatt ccgattcacc
     1501 gccgccacgg gatgtgttgg ttctgatttg cggcgcattg ccgcccaggt cagacggttg
     1561 cgtgctggag ttctcctgag gcaaaagaca ttcattgcaa gggggagtca cgaagatcgc
     1621 ctatggcgcc tcggccagcc atgaacatga acaacggccg cccacgaggc gtggcgggag
     1681 cgtcgtatca tggcaggata ctaacgaatc cgacactgcg ccgggagatg cgccagcacg
     1741 cccgctccgg tccgtgccgg agcggctgag gtgattcgcc acaagcgggg ggcaacacgg
     1801 cggcagggcc tggtcatgct tctggccctc gttgggctgc tcgccccgat tctccaggcg
     1861 cacgcgcagg cgtcgctgga aagcgccatc gaggcaactt tcctgctgaa attccccccc
     1921 ttcatcacct ggcaccaggg gctgccgccc gactccttca ccgtctgcgt gctgggtgat
     1981 tcgccggtca ccggcctgtt gcggcaggcc gccgccgggc tgtcggttga ccagcacccg
     2041 gtgcgggtac ggcacgtttc ggcggcaacc gcatcgagcg gctgccagga gttgtatctg
     2101 gccggcagcg acccgacagc gatcgccacg gcgctggccg cgacccgcgg cacaccggtg
     2161 ctgaccgtga ccgacgggca gaccgatccc accgcgaagg gaatcctcaa ttttgtgctc
     2221 accgacgggc atgtgcgttt cgaggtggat ctcaacgccg ccgcggcaaa cggcatctcg
     2281 atcagttcca agcttttgag tatcgcggtc cacgtcaatg gcagcgcgca ctaactggag
     2341 gccaggcatg tcgagactat ggagggtgac accggccatc gccgccgcca cagtggtggt
     2401 gctgctgctc gccgcggtgt tcctcggtca ctacatcgat cgtgccaacc tggcgcagaa
     2461 ggtgaccgag gcgaccgagc aggcgaagat cctggcatcc accgcctcgg ccgcggtggc
     2521 cttcagcgac cggcaggccg ccggcgaata ccttggcgcc atacgcgcca atcccgaggt
     2581 cgaatgggcc gcgatctatg acgcgaaggg gcagctcttc gccagcttct cccgcagcga
     2641 cggccgtccg ccggacgccg cgccgccgct gcggccgccc tttgtcgaga gcggccacat
     2701 caatgtgctg gtgccgatcg tgcaaaaggg caaccggctg ggcaccgtct atctgcacac
     2761 cccgaccgag ccggtggaac gccggctgga acgctacgcc gtaatcgggc tgttcgcggt
     2821 gatggccgcg ctgattgtgg cagtgctcgg tatcgcgcaa ggcgcactga cccgggcgaa
     2881 caccgaactc gcccggcgcg ccggcgaact ggcgctggcc aacaccagcc tgcgcgaaca
     2941 gatcgaacag cgggagaagg tggaggaagc gctgcgccag gcgcagaaga tggaagcgat
     3001 cggccaactg accggcggtg tggcgcacga cttcaacaac ctgctgcagg tgatcctggg
     3061 caatctggag cggctgcgcc gccgggcact ggacccgatg gaggcgcacc ggctgatcga
     3121 cgcggctctg cgcggcgccg aacgcgccgc cacgctcacc cagcgcctgc tggccttctc
     3181 gcgccgccag cccctggcgc ccaaggtgct ggaggtgaac aagctggtca ccggcatgtc
     3241 cgacctgctc agccgcaccc tgggcgagaa catccgcatc gagaccgtgc tggccggcgg
     3301 cttgtggcgc gtgtcggcgg acgagaacca gttggagagc gcgctgctga acctggcggt
     3361 gaacgcgcgc gatgccatgc agggcgccgg caagctgacc atcgagaccg gcaacgccta
     3421 tctcgacgat gcgtacgtcc gcaacgagga ggaagtgaag ccgggccagt acgtgctgat
     3481 cgccgtatcc gataccggca gcggcatgag caaggacatc ctggaccggg tgttcgaacc
     3541 gttcttcacc accaaggaga tcggccaggg caccggcctg gggctgtcga tggtgtacgg
     3601 cttcgtcaag cagtccggtg gccacgtgaa gctgtacagc gagctcggtc agggcacgac
     3661 ggtgaagctc tatctgccgc gcctgctgac cgagcctgcg caggcccagg cgccgcaatc
     3721 cgccgccagc ctgccacgtg gtcaccgcaa tgaactggtg ctggtggtag aggacgagga
     3781 cgatgtccgg gcgctgaccg tcgatacgct gcaggaactc ggttacgacg tgctgcaggc
     3841 gatggacggc gcctcggcgt tgcgcctgct ggagagcgaa cccggcatcc ggctgctgtt
     3901 caccgatgtc ggattgcccg gcggactgaa cggccggcag cttgccgacg cggcccgcca
     3961 gacgcggccc gggctgaaag tgctgttcac caccggctat gcgcgcaatg ccatcgtgca
     4021 tcacggacgg ctggatccgg gcgtgcagtt gatcagcaag cccttctccg acaccgacct
     4081 ggcgcacaag gtgcggcaga tgctggacgg gactgaagtg tagccgagcg ccccgcgctt
     4141 tgcaggacgc ggctcgtgcg caatgctgta cccgcagcat cgccgacagg aaccgacagg
     4201 atgacagaca gcacttggcg cgaccgcgcc ggtacccgct tccagtgtga gaagtaccag
     4261 gcgaccagcc ggcgcggcat ggtggtcacc aaccatccgc tggcctccgc cgccggggtg
     4321 gagatgctag cggctggcgg caacgcgatc gacgccgcaa tcgccgcctt cttcaccctg
     4381 acggtggtgg agccgatgat ggtcggcgtg ctcggcggtg gcatggcgca tatccgcctg
     4441 tccgacggca accacgtggt gctggacggc cagagcatgg cgccgctcgc caccggcccc
     4501 accacctaca agcccgatcc gcaagccgcg cccggcacca tggacgcgat cggccgcaag
     4561 aacgcgctgg gccccagcgc agtggcgacc ccgggcaacc tgatgggctg gtgccaggca
     4621 ctggaacgcc acggccggtt ttccctggcc gatgtcatgg agccggcgat ccgccatgcc
     4681 tcccgcggct tccgtgtcac gccgtacctg aatgaatgtg tcaccgattg cgccgcggac
     4741 ctggcgctgg accgggagat cgccgcgctg tacctgccga acggcgcacc gatccagccc
     4801 ggcgcacggc tggtcaccgg cgactacgcc gaaaccttgc gcagcatcgc gcgcgaaggc
     4861 cccaacctgc tgtacggcgg tgcgctgggg cggctctaca ccgaccacat ggcacgcaac
     4921 ggcggctacc tgggcatgga cgacctgacc cgctaccgca ccatcgatcg ccaggcgctg
     4981 cgcggcacct atcgcggcca cgagattgtc ggcccgccgc cgccgtcttc cggaccgctg
     5041 cacatcatcc agatgctgaa tatcctggaa ggctacgaca tccgcgcgct ggggttcggc
     5101 agcgccgata cgctgcacct gctggccgaa gtgctgaaga tcgccttcgc cgatcgcacg
     5161 gccgcgaccg ccgatccggc cttcgttccg gtgccggtgg cgaagctgct gtcgaaggac
     5221 tacgcggagc aacgccggtc ccgtatcgac ctgcagcggg cgcagcaatg gagcgcggag
     5281 gtggcgcccg gcgaaggcgc gcacaccacc cacctgaccg tggcggacgg tgacggcaac
     5341 gtcgtcgcca gcacgcaaac catcaacagc ctgttcggcg cgcggtacat cgtgcccggc
     5401 accggcatga tcccgaacaa ctacatgttc gtgttcacgc cgcaccccgg ccaggcgaat
     5461 tccgtggcgc ccggcaagcg gatcacgt
//
LOCUS       DASSZH010000629         6549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_526337, whole genome shotgun sequence.
ACCESSION   DASSZH010000629 DASSZH010000000
VERSION     DASSZH010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526337"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VFN42_13580"
     CDS             complement(<1..1041)
                     /locus_tag="VFN42_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET8997693.1"
                     /translation="MPSSTNLPPPDPAWPAATPEDAGFSPAALQEAVRFATDHEIGWP
                     RDLRAHIESGYFEAPPDNEILGPIRPRGPANGLIVRGSRVVARWGNTRQVDFTFSVAK
                     SYLSLLAGIAIADGLIQELDEPVARTVDDGGFAGPHNGTITWRHLLQQTSEWEGALFG
                     KSDIIDRNRNLSVEGKGRKGDARPLQPPGTHWEYNDVRVNRLSLALLRRFRRPLPEVF
                     ADRIMRPIGASSDWRWHGYRTSQVEIDGRLIESVSGGSHWGGGVEIHAEDQARVGLLM
                     LHQGAWNGQPILPERWIAESLTPCALNANYGLLWWLNTGCTRYPSAPAASFFASGAGG
                     NITFVSPEHDIVA"
     gene            complement(1089..1964)
                     /locus_tag="VFN42_13585"
     CDS             complement(1089..1964)
                     /locus_tag="VFN42_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8997694.1"
                     /translation="MSTTTLAAPVFVPQSPRRLAVRRFLRRRVAVAGLVVILVFVAVA
                     VFAPLIAPYDPIATSWTLIRKAPSAAHWMGTDENGRDVLSRVIFGARASLMAGVVSVL
                     IAAGIGVPAGLLAGFAEGWIDAILSRIVDAMLACPFLILAIALAAFLGPNLTNAMIAI
                     GVSTAPRFMRVARAATLDAASNEYVEAARAIGNPGWRVAVRHVLPNIVPPVLVQGTLA
                     IAQAIIAEASLSFLGLGEQPPAASWGSMLNSAQRFLTQAPWLAVFPGLAIFLVVLSFN
                     LVGDGLRDALDPRGK"
     gene            complement(1975..2922)
                     /locus_tag="VFN42_13590"
     CDS             complement(1975..2922)
                     /locus_tag="VFN42_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8997695.1"
                     /translation="MRGHLGTRIAQIIPTLLLVSVLVFCLQQLMPGDPALIMAGEEGN
                     DVHVIAQIRAELWLDRPLPVQYAHWMGNVLQGNLGYSWRLREPVTELIAQKLPVTLQL
                     SVMAFIIAVLIGVPAGVIAAVYRNGFWDYVANAVGLAGLSTPTFWLGIMLILLVSVQL
                     GWLPPSGYVPLTVDWQQSLATTVMPAFVLGNAIAAVLMRHTRSAMLTALDQDFVRTAR
                     AKGLREYVVVLRHALRNALIPVVTLGALELGTLFSGAVLTEQVFSIPGFGKLIVDAVF
                     NRDYPVVQGVVLVTAFLYVVLNLLADVLYVVINPRLRGA"
     gene            complement(2923..4425)
                     /locus_tag="VFN42_13595"
     CDS             complement(2923..4425)
                     /locus_tag="VFN42_13595"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8997696.1"
                     /translation="MRRFLLAAGLLAAVTSTASAQTLRIALRQDLDVLDPTYATTYVG
                     RIVFAGLCDKLFDIDAKLNIVPQLATGYKWQDDKTLILNLRPGVKFQDGEPVDAAAVK
                     FTLEHYLTTKGSYRRSEISEIDHIDVIDPTTVRIVLKHPSGAFLAQLTDRSGMILPPK
                     VVEQEGKNFSQHPVCSGPYKFVERVPQDHVTLERFAGYWNAKAIHFDKVIYQVLVDSS
                     VRLANLKSGAIDLAEYIAPTDAAAVKADPHLRLVVTPSLGYFGISNNLANGPQADNPY
                     GKSALVRQALDLAIDRKALVSVVFSNMYTPSVEPVPASSPFYDPSIKVPERNVAKAKA
                     LLKQAGVKLPVHLELLAFNNPDILQEAEVIQSMAAEAGFDIKIRAMEFASSLQAGRQG
                     QFQAYLIGWSGRPDIDGNTYQFLHSGQGNNDGHYSNPTVDKLLEEGRGTVALAKRKPL
                     YAAMMQQERKDLPITYLYTPNNIVGMSAKLQGFRPIPDGMIRLQGLEMAK"
     gene            4671..5648
                     /locus_tag="VFN42_13600"
     CDS             4671..5648
                     /locus_tag="VFN42_13600"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET8997697.1"
                     /translation="MISVRQNGSAARARWHWGQDAGTLSFGGDGGSPQGRRRLVGALR
                     RPTAPHLAPAILAHAALLAGVIWLTTRPSQPPEPLPESNFAMVFTLPSASPPPVATPQ
                     PVQQEPPPVPQTAAPPQPQPVTAPPPPPALPQPAPVAAAPPPPAAQSAVAPPPPAAQS
                     TVAPPPPPPPPAPPQTAIAAPDLPLPPPPAPVPPPRAVAARPTRAPVVPNWHPTAPAS
                     VAVASTPVTAPMPSIPPRLIAAIAGDRPPIYPELARRRGEQGRVLLLVHVGADGMPVA
                     VTVNRSSGYSSLDHAAIAAVEQWRFAPAMQGGRPVAAVAEIPVQFHLSD"
     gene            5661..>6549
                     /locus_tag="VFN42_13605"
     CDS             5661..>6549
                     /locus_tag="VFN42_13605"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HET8997698.1"
                     /translation="MRITGRPGQIAATLCLTLLLAPEAFAQAETGATVLPPVTVVAPT
                     PLLGSGIDRGKAPNSVNVLNRQDIARSGVPSMLHALEQQTPGVSLDDAQGNPFQPNLE
                     YRGFVASPLDGNPQGLAVYLNGVRFNAAFSDAVNWDLLPSIAIDQVNLEGANPVFGLN
                     ALGGALSIQMKNGFTWKGGQAELYGGSFGTVAGSMQYGRQSGSESTYLAVNIDHQRGW
                     RQLNSSDVRQAYGDIGWRSDTAELHWNVLLADNLLNGPGTVPVQFLAADRTATFTAPN
                     RTTNRYALSSLSGTWSLTDQ"
BASE COUNT         1120 a         2273 c         2166 g          990 t
ORIGIN      
        1 ggcgacgatg tcatgctccg gcgacacgaa ggtgatgttg ccgcccgcgc cggaggcgaa
       61 gaagctggct gccggggcgc tgggatagcg cgtgcagccg gtgttcagcc accacagcag
      121 gccgtaattg gcgttcagcg cgcagggcgt gagcgactcc gcgatccatc gttccggcag
      181 gatcggctga ccgttccagg ccccctggtg caacatcagc aggccgacgc gcgcctggtc
      241 ctcggcatga atctccacgc cgccgcccca atggctgccg cccgataccg attcgatcaa
      301 ccggccgtcg atttccacct gcgacgtgcg gtagccatgc caacgccagt cggatgacgc
      361 gccaattggc cgcatgatac ggtccgcaaa gacctccggc agcggacggc ggaaccggcg
      421 caacagcgcc aggctgaggc ggttcacccg cacgtcgttg tattcccaat gcgtccccgg
      481 cggctgcaac gggcgcgcat cgcccttgcg gcccttcccc tcgacggaga ggttgcggtt
      541 gcggtcgatg atatcggact tgccgaacag cgcgccttcc cactcggagg tctgctgcag
      601 caaatgccgc caggtgatgg tgccgttgtg cggaccggca aagccgccat cgtccacggt
      661 gcgcgccaca ggctcatcca gttcctggat cagcccatcg gcgatggcga tgcctgccag
      721 gaggctgaga tagctcttgg cgacggagaa ggtgaagtcc acctgccgcg tgttgcccca
      781 gcgcgcgaca acccgactgc cgcggacaat caacccgttc gcggggccgc gcggacggat
      841 cgggccgagg atttcgttgt ccggcggcgc ttcgaagtag ccggattcga tatgcgcgcg
      901 caggtcgcgt ggccagccga tctcatggtc ggtggcgaag cgcacggcct cctgcaaggc
      961 ggcgggagaa aagcccgcat cctccggcgt cgcggccggc caggcgggat cgggcggcgg
     1021 caggttggtg gaggacggca tgcagtacgg ctccggcggc cggttgcact cggaaaggat
     1081 cagcggcttc acttcccacg tggatcgagc gcgtcgcgca gcccgtcgcc cacgaggttg
     1141 aatgacagca ccaccaggaa gatcgccaat cccgggaaga ccgccagcca gggcgcctgc
     1201 gtcaggaagc gctgggccga gttcagcatg gagccccagg acgcggcagg cggctgctcg
     1261 cccaggccaa ggaacgacag cgacgcctcg gcgatgatcg cctgcgcgat ggccagcgtg
     1321 ccttgcacca gcacgggcgg gacaatgttc ggcagcacgt gccgcaccgc gacgcgccag
     1381 cccgggttgc cgatggcgcg cgctgcctcc acgtactcgt tgctcgccgc atccagcgtc
     1441 gccgcgcgcg ccacgcgcat gaagcgcggt gcggtggaca cgccgatcgc gatcatcgcg
     1501 ttggtcaggt tcggccccag gaacgccgcc agcgcgatcg ccaggatcag gaacgggcag
     1561 gcaagcatgg catcgacgat ccggctgagg atcgcatcga tccagccttc cgcgaagccc
     1621 gccagcaacc ccgccggaac gccgattccg gcggcgatca gtaccgacac cacacccgcc
     1681 atcaggctgg cgcgggcgcc gaagatcacc cgcgacaaca catcgcggcc gttctcgtca
     1741 gtgcccatcc agtgcgctgc ggacggcgcc ttgcggatca gcgtccagga ggtggcgatc
     1801 ggatcatatg gcgcgatcaa tggggcgaac accgcgacag cgacgaacac caggatgacg
     1861 accagcccgg caaccgcgac gcggcgccgc aggaaccggc gcaccgccaa ccggcgcggg
     1921 ctttgcggga cgaagactgg ggcggcaagc gtggtggtgc tcatgacacc tgtttcatgc
     1981 cccgcgcagc cttggattga tcacgacgta cagcacatct gccagcaggt tcagcaccac
     2041 gtagaggaat gcggtgacca gcaccacgcc ctgcaccacc ggatagtccc ggttgaacac
     2101 cgcatcgacg atcagcttgc cgaagcccgg aatgctgaac acctgctcgg tcagcaccgc
     2161 gccggagaac agcgtgccca gttccagcgc gcccaaggtc accaccggga tcagcgcgtt
     2221 gcgcaaggca tggcgcaaca ccaccacata ttcccgcaaa cccttggcgc gtgcggtacg
     2281 gacgaagtcc tggtccagcg ccgtcagcat tgcactgcgc gtgtgccgca tcagcaccgc
     2341 ggcgatggcg ttgcccagca cgaaggccgg catgaccgtg gtggcgagcg actgctgcca
     2401 atccacggtc agcggcacgt agcccgacgg cggcagccag cccagttgca ccgataccag
     2461 caggatcagc atgatgccga gccagaaggt gggcgtggaa aggccggcaa gaccgacggc
     2521 gttggccaca tagtcccaga acccattgcg gtacaccgcc gcgatcacgc ctgccggcac
     2581 gccgatcagc accgcgatga tgaaagccat gacgctgagc tgcagcgtca ccggcagctt
     2641 ctgggcaatc agctcggtca ccggttcgcg cagccgccag gaatagccaa ggttaccctg
     2701 cagcacgttc cccatccaat gcgcgtactg caccggcagc ggccgatcca gccatagctc
     2761 cgcacggatc tgcgcaatga cgtggacgtc gttgccctcc tcccccgcca tgatcaaggc
     2821 cggatcgccg ggcatgagct gctgcaggca gaagacgaga acgctgacca gcagcagcgt
     2881 cgggatgatc tgcgcgatgc gcgtaccgag atggccgcgc acttacttcg ccatctccaa
     2941 cccctgcaaa cggatcatcc cgtcggggat cggccggaag ccttgcagct tcgcgctcat
     3001 ccccacgatg ttgttcggcg tgtacaggta ggtgatcggc aggtccttgc gctcctgctg
     3061 catcatggcg gcgtacaggg gcttgcgctt ggccagcgcg accgtcccgc ggccttcctc
     3121 cagcagcttg tcaaccgttg ggttgctgta gtggccgtcg ttgttgccct gcccggaatg
     3181 caggaactga taggtgttgc cgtcgatgtc cggccggccc gaccagccga tcaggtaggc
     3241 ctggaattgg ccctgccgtc ccgcctgcag cgaggaggcg aattccatcg cccggatctt
     3301 gatgtcgaat ccggcttcag ccgccatcga ctggatgacc tcggcctcct gcaggatgtc
     3361 cgggttgttg aacgccagca gttccaggtg caccggcagc ttcacgccgg cctgcttcaa
     3421 cagcgccttc gccttggcga cattgcgttc cggcaccttg atcgatggat cgtagaacgg
     3481 cgaactcgcc ggcaccggct ccacgctcgg cgtgtacata ttgctgaaca ccacgctgac
     3541 cagcgccttg cggtcgatcg ccaggtcaag cgcctgccgc accagtgcgc tcttgccgta
     3601 cgggttgtcc gcctgcggcc cgttcgcgag attattgctg atgccgaaat aaccaagcga
     3661 cggcgtgacg acgaggcgca gatgcgggtc ggccttcacc gccgcagcgt cggtcggcgc
     3721 aatatattcc gccagatcga tcgcgccgga tttcaggttg gccaggcgca ccgaggagtc
     3781 caccagcacc tggtagatca ccttgtcgaa atggatcgct ttcgcgttcc agtagccggc
     3841 gaagcgctcc agcgtgacgt ggtcctgcgg cacgcgttcg acgaacttgt agggaccgct
     3901 gcacaccgga tgctgggaga agttcttgcc ttcctgctcc accaccttgg gcggcaggat
     3961 catgcccgaa cggtcggtca gctgcgcgag gaaggcaccc gacggatgct tcagcacgat
     4021 acgcacggtc gttggatcga tcacgtcgat atggtcgatc tcgctgatct cgctgcgccg
     4081 gtagctgccc ttggtggtca gatagtgctc cagcgtgaac ttcacggcgg cggcgtcgac
     4141 cggctcgcca tcctggaatt tcacgccggg gcgcagattc aggatcagcg tcttgtcgtc
     4201 ctgccacttg tacccggtgg cgagctgcgg cacgatgttc agcttcgcat cgatgtcgaa
     4261 cagcttatcg cacaggccgg cgaagacgat gcggccaacg taggtcgtgg cataggtcgg
     4321 atcgaggaca tccaggtcct gccgcaaggc gatgcgcagc gtctgcgcgg aagcagtgga
     4381 ggtcacggca gccaacagac cggcagccag caggaagcgg cgcatacggt agtcccccgg
     4441 atcatcggtt tgattgcaac gaattgtggg cgcagcccgg accggaggca agccggaatc
     4501 gtcgccggtg tcggtgacgc gggccgccag gcttcggata cccggttgcg atgcgggtga
     4561 gatgcgggtt gcccgatgca ggctggcccg cctaagtttt tgggtcgcgc cgcaccgtgg
     4621 tttatcctcc aggcagagag gggaggcggc ggggaacgta gggaggcgca gtgatctcgg
     4681 tccgccagaa cgggtcggcc gcgcgggcgc gttggcattg gggacaggat gccggcacgc
     4741 tcagcttcgg gggcgacggc ggctcgccgc agggacggcg gcgcctggtg ggcgcacttc
     4801 gacgccccac cgccccgcac ctggcgccgg cgatactggc gcatgcggca ctgctggccg
     4861 gcgtcatctg gctgaccacc agaccgtccc agccgccgga accgctgccg gaatccaact
     4921 tcgccatggt atttacgctg ccgagcgcca gtccgccgcc ggtcgccacg ccgcagccgg
     4981 tgcagcagga gcctccgccg gtcccgcaga ccgccgcacc gccgcagccg cagcccgtga
     5041 cggctccccc accgccgcct gcgctgccac agcccgcgcc ggttgcagcc gcgccgccgc
     5101 cgccggccgc gcagagcgct gtcgccccgc cgccgccagc cgcgcagagc accgtcgccc
     5161 cgccaccacc accaccccca ccggcgccgc cgcagaccgc cattgcggcc ccggatctcc
     5221 cgctgccccc cccgccggcg cccgtcccgc cgccgcgcgc agtggcggca agacccaccc
     5281 gcgcacccgt ggtgccgaac tggcatccca cggcgccggc ctcggtggcc gtcgccagca
     5341 caccggtcac cgcaccgatg ccaagcatcc cgccgcggct gatcgctgcg atcgccggcg
     5401 accggccgcc catctatccc gagctggcgc ggcgccgcgg cgaacagggg cgcgtgctgc
     5461 tgctggtcca tgtgggcgcg gacggtatgc cggtggcggt cacggtgaat cgctccagcg
     5521 gctacagcag cctggaccat gcggcgatcg ccgcggttga gcagtggcgc ttcgcccccg
     5581 ccatgcaagg cggccgtccg gttgcagccg tggcggagat tcccgtccag ttccacctgt
     5641 ccgactgagc aagggatccg atgcgcatca ccggccggcc tggccagatc gcggcaacac
     5701 tctgcctgac cctgttgctg gcgccggagg cgttcgccca ggccgagacc ggcgccaccg
     5761 tgctgccgcc ggtcaccgtg gtggcgccca cgcccttgct gggctccggc atcgaccgcg
     5821 gcaaggcgcc gaacagcgtc aacgtgctga accggcagga catcgcgcga tccggcgtgc
     5881 ccagcatgct gcatgcgctg gaacagcaga cgccgggcgt gtcgctggat gacgcgcagg
     5941 gtaacccgtt ccagccgaac ctggaatacc gcggcttcgt cgcctctccg ctggacggca
     6001 atccgcaggg actggcggtg tatctgaacg gggtgcggtt caatgccgca ttctccgacg
     6061 cggtgaactg ggacctgctg ccctcgatcg caatcgacca agtgaacctg gagggggcca
     6121 acccggtgtt cgggctgaac gcgctggggg gcgcgctgtc catccagatg aagaacggct
     6181 tcacctggaa gggcggccag gcagagctgt atggcggatc gttcggcacg gtggccgggt
     6241 caatgcagta cggccggcag tcgggcagcg agtccacgta tctggccgtc aacatcgacc
     6301 accagcgggg ctggcggcag ctgaacagtt ccgacgtgcg gcaggcctat ggcgacatcg
     6361 gctggcgcag cgacacggcg gaactgcact ggaacgtgct gctggccgac aacctgctga
     6421 acggaccggg caccgtgccg gtgcagttcc tggccgccga ccgcaccgcg accttcaccg
     6481 cgccgaaccg caccaccaac cgctacgcgc tgagcagcct gtccggcacc tggagcctca
     6541 ccgaccaga
//
LOCUS       DASSZH010000630         2112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_134903, whole genome shotgun sequence.
ACCESSION   DASSZH010000630 DASSZH010000000
VERSION     DASSZH010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134903"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /gene="fabD"
                     /locus_tag="VFN42_13610"
     CDS             complement(<1..915)
                     /gene="fabD"
                     /locus_tag="VFN42_13610"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET8997699.1"
                     /translation="MNANIDRSFTGPRGDTPASHPDPRLAFVFPGQGSQAVGMLAEIA
                     AQHPEVVATFAEASAGAGIDLWQLAQQGPEARLNRTEHTQPALLAAGIAVWRVWNKLG
                     GAQPALLAGHSLGEYTALVAAGALDLAEAAALVAERGRLMQEAVPEGEGAMAAVLGGD
                     DALIAEVCAEVAEGQVVAPANFNAPGQTVIAGQADAVARALARLAERGVRKSVRLPVS
                     VPSHCELMREAAARLAERMASVSWRMPQVPVLQNVEARAYGSIEYIRAALERQLYMPV
                     RWSESVQVLAAQGVTRVAECGPGKVLAGL"
     gene            complement(1050..2018)
                     /locus_tag="VFN42_13615"
     CDS             complement(1050..2018)
                     /locus_tag="VFN42_13615"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HET8997700.1"
                     /translation="MYARIAGTGSYLPSHVVTNADLEHLVETSDEWIRTRTGIRERRK
                     AAEGETTGDLGFHAAVAALESAGMAASELDLIIVGTTTPDLIFPSTACLIQHRLGANG
                     CAAFDVNAACSGFVYALGVADNFIRAGTSRKALVVGSETLTRMVDWGDRSTCVLFGDG
                     AGAVVLEASAEPGILTSKLHADGGHKDLLYNPVGVSVGFRPGEANAGVCIRMSGNEVF
                     KVAVRTLDRIVGETLEAAGMHESQVDWLIPHQANLRIIEATARRLGLPMERVIVTVDR
                     HGNTSSGSVPLALDEAVRSGKVQRGQTLLLEAFGGGFTWASALIRY"
BASE COUNT          322 a          755 c          704 g          331 t
ORIGIN      
        1 cagtccggcc agcaccttgc ccggtccgca ctcggcgacg cgcgtgacgc cctgcgcggc
       61 cagcacctgc accgactcgc tccagcgcac cggcatgtac agctggcgct ccagcgccgc
      121 gcgaatgtac tcgatgctgc cgtaggcgcg cgcctcgaca ttctgcagca ccggcacctg
      181 cggcatacgc caggataccg acgccatgcg ctcggccaaa cgcgccgccg cctcgcgcat
      241 cagttcgcaa tgcgagggca ccgacaccgg cagacgcacg gatttgcgca cgccgcgctc
      301 ggccagacgc gccagtgcgc gcgccaccgc gtcggcctgg ccggcgatca cggtctgtcc
      361 gggcgcattg aagttggccg gcgccaccac ctggccttcg gccacttcgg cgcacacttc
      421 ggcgatcagc gcgtcatcgc cgcccagcac ggccgccatg gccccctcgc cttcaggcac
      481 ggcctcctgc atcaggcgac cgcgttcggc caccagcgcg gcggcctcgg ccagatccag
      541 tgccccggcc gcgaccagcg cggtgtactc gcccaggcta tgtccggcca gcagcgcagg
      601 ttgcgcgccg ccgagcttgt tccatacccg ccacaccgcg atgcccgccg ccaacagcgc
      661 cggctgcgtg tgctcggtac ggttcagtcg cgcctccggt ccctgttgcg ccagctgcca
      721 cagatcgatg ccggcgcctg ccgaggcttc ggcgaaggtg gcgaccactt ccgggtgctg
      781 cgccgcgatt tcggccagca tgcccacggc ctgcgaaccc tggcctggaa agacgaaggc
      841 caggcgtgga tccgggtgtg acgcaggcgt atcgccacgg ggcccggtaa atgacctatc
      901 gatgttcgcg ttcatgcttg tgcgatcgct gagattctaa aacgtgtttg caaatcgtgg
      961 ccgctgccgg cagccctgcc ttgggatcac gggttccggc cggtctggtc acggtgcgct
     1021 gcatctgcga tcccggatcg gaaggcacgc tagtaacgga tcaacgccga tgcccaggtg
     1081 aagccgccgc cgaaggcttc cagcagcagc gtctggccac gctgcacctt gccggaacgc
     1141 acggcttcat ccagcgccag cggcaccgag cccgaggacg tgttgccgtg gcggtccacg
     1201 gtgacgatca cgcgttccat gggcaagccc aggcgcctgg cggtggcctc gatgatgcgc
     1261 aggttggcct ggtgcgggat cagccagtcg acctgcgact cgtgcatgcc ggcggcttcc
     1321 agcgtctcgc cgacgatgcg gtcgagcgtg cgcacggcga ccttgaacac ctcgttgccg
     1381 ctcatgcgga tgcagacgcc ggcgttcgcc tcacccggcc ggaagcccac ggagacaccc
     1441 accgggttgt agagcaagtc tttgtgcccg ccatcggcgt gcagcttgct ggtcaggatg
     1501 ccgggctccg cgctggcttc cagcacgacg gcgccggcgc cgtcgccgaa cagcacgcag
     1561 gtggagcggt cgccccagtc gaccatgcgc gtcagcgtct cggagcccac caccagcgct
     1621 ttgcgcgacg tgccggcgcg gatgaagttg tcggccacgc ccagcgcgta aacaaagccc
     1681 gagcacgccg cgttgacgtc gaaggccgcg cagccattcg cgcccagccg atgctggatc
     1741 aggcaggcgg tggatggaaa aatcaggtcg ggcgtagtgg tgccgacgat gatcagatcg
     1801 agttcggatg cggccatgcc cgcgctttcc agcgcggcga cagcggcatg gaaacccagg
     1861 tcgccggtgg tctcaccctc ggcggccttg cgccgctcac ggatgccggt gcgggtacgg
     1921 atccactcgt cgctggtctc gaccaggtgt tcgagatcgg cattggtgac tacgtgactg
     1981 ggcaaatagc tgcccgtgcc ggcgatgcgt gcgtacatct ggcctgtccc ctgagcgttg
     2041 cggcgcgaac gcggccgtgc atcggccacg ggcgatgggc agccgactca gtcttcgtcg
     2101 acggcggtgg cg
//
LOCUS       DASSZH010000631        10181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_612178, whole genome shotgun sequence.
ACCESSION   DASSZH010000631 DASSZH010000000
VERSION     DASSZH010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 10181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10181
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612178"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VFN42_13620"
     CDS             <1..180
                     /locus_tag="VFN42_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997701.1"
                     /translation="PSSIYATEARATQEETRALHHLPNNGHQRRMRHPSALLPALAAQ
                     AAAHTHRARNRLRRR"
     gene            277..1515
                     /gene="hisS"
                     /locus_tag="VFN42_13625"
     CDS             277..1515
                     /gene="hisS"
                     /locus_tag="VFN42_13625"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HET8997702.1"
                     /translation="MPSELQPPRGTHDLIGEEQRRHAHVVDTARRIAATYGFDEWSTP
                     IFEDTRVFARTLGETSDVVTKEMYSFEDRGGDSITLRPEATAGICRALVSNGLTQSLP
                     QKVFYTGPMFRYERPQKGRYRQFHQIDIELIGSAEPLADAEIIACGWHILQELGVAGE
                     TILEINTLGDKASRQAYRDALVGYFNEHQASLSSDSLSRLQRNPLRILDSKDENDRRI
                     VANAPVIAGHLTDAAAAFYAAVRRYLDAFKVPYRENPRIVRGLDYYGHTAFEFVTSKL
                     GAQGTVMGGGRYDGLVEEMGGPPTPAVGLAAGVERLAMLLQDPPPPPAPVAVVPVGEA
                     AELAALDALQSLRAAGIRSEMALRGNLRRRMERANRIGARAAVIIGQDDIAQGVAQVK
                     DLRTGSQEAVALAALPDRLR"
     gene            1512..2570
                     /gene="prfA"
                     /locus_tag="VFN42_13630"
     CDS             1512..2570
                     /gene="prfA"
                     /locus_tag="VFN42_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET8997703.1"
                     /translation="MNLTHKLDRIAARADELQAMLAEGATGETYVKASKEFSELEPVV
                     ARIGELRAAEKAQAEAEAMLADPEMRELAEVELHSLKQRIPVLEHELQLALLPKDEAD
                     ARSAILEIRPAAGGDEAALFAAELFGMYQRYAESRGWRFEILEYNDTGLGGLKEGIAE
                     INGRGVFARLKFESGVHRVQRVPATESQGRIHTSTVTVAVLPEAEEVDVQIDEGDLRI
                     DVYRASGAGGQHVNKTESAVRITHLPSGIVVAMQEEKSQHKNRAKAMTILRARLYEQQ
                     RAAAHATRAADRKSQVGTGDRSERIRTYNFPQGRVSDHRINLTLYKIDRVMQGELDEF
                     IDALTAEDQAARLAAAEA"
     gene            2776..3642
                     /gene="prmC"
                     /locus_tag="VFN42_13635"
     CDS             2776..3642
                     /gene="prmC"
                     /locus_tag="VFN42_13635"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET8997704.1"
                     /translation="MAAAAGRLRAAGTDSPRLEARLLLAHILGLTPTALLRDPSTRVP
                     PEAQARFAALVDRRAGREPLAYILGHREFWSLDLAVSPATLIPRPDSETLVAAARSAF
                     AGRAPPHSILDLGTGTGALLLAALSEFPAAWGVGVDRVPAAARLAAANAAALDLAPRA
                     VFLAGIWADALAARFDLVLCNPPYIPTSELQALMPEVARHEPASALDGGADGLDAYRL
                     IIPELPRLLSPQGVAVLELGAGQAQAVAGLARQAGLASGTQDDLAGVPRALLLANRSA
                     MKKPFGTTKWAV"
     gene            3869..4345
                     /locus_tag="VFN42_13640"
     CDS             3869..4345
                     /locus_tag="VFN42_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4167 domain-containing protein"
                     /protein_id="HET8997705.1"
                     /translation="MNMKRMRGRGHRSGGGGGGGGGMRHHGGAGGGGIPLNRNHVFDS
                     NGPDVRIRGTAQQLSEKYLQLGRDATSSGDRVMAEAYFQHAEHYFRILNAMNQAAQQG
                     QPSGQGGQRRHYQGNGEDGQSSGGSSESEESDSRAPGLGDQPESREIPIAAAPPEA"
     gene            4433..5032
                     /gene="pdxH"
                     /locus_tag="VFN42_13645"
     CDS             4433..5032
                     /gene="pdxH"
                     /locus_tag="VFN42_13645"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011253501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HET8997706.1"
                     /translation="MADSAPLVTDPFARFRAWMAEAEASEPSDPNAMIVATATPDGHP
                     SARAILLKGVDDRGFVFYTNKESRKADELAANAQIALLFHWKSLARQIRIEGRVEHVT
                     GAEADAYYASRPRISRLGAWASRQSRPLDERATLERRLAEYEAKYPGEDIPRPEYWSG
                     YRVLPDRFEFWQNMPFRLHDRTTYVRDAQGGWSIGKLFP"
     gene            complement(5039..5449)
                     /locus_tag="VFN42_13650"
     CDS             complement(5039..5449)
                     /locus_tag="VFN42_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997707.1"
                     /translation="MPRPALLAALLLFATPALAQVRPSAPLGPSGQNVRSFSLKNMSH
                     APVVFARARMTDGKIRALTYSPIQPGEARQIVVPRKECLADVVVKLNNGRTLKAPNLN
                     DCRSNQLVVRDRGINVLSADVRQLRRGKLPPSSG"
     gene            complement(5512..6843)
                     /locus_tag="VFN42_13655"
     CDS             complement(5512..6843)
                     /locus_tag="VFN42_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010514649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HET8997708.1"
                     /translation="MSRLVIVSNRVPTPRERNQAAGGLAVALKDAVAAQETLWFGWSG
                     RTCHGAPGAPQVTHARRLTQATIDIPASLFGGYYEGFSNGTLWPLMHWRAGLAEFSRE
                     NFEAYMAVNEQFADALAPLLRPGDLVWVHDYHLIPLGRMLRERCERIRLGFFLHIPFP
                     PAPLFETLPQSQRLLKAFGAYQLVGTQTKADAMYLAQALRDAEVGALVEAFPIGIDAA
                     SFANAAVHAASGPEVERLHGSLGDRTLILGVDRLDYTKGIPERFRGYAKLLRRFPQHR
                     NRVTFLQVAPVSRGAIGRYRALRRELDELAGRINGENAEFDWTPLRYITRAIPRTTLA
                     GFHRLARVGLVTPLRDGMNLVAKEFVAAQDPENPGALVLSQFAGAAEELDGALLVNPH
                     DPDELAEVVDAALSMPLEERQRRWRSMHETVQRGTARAWARSFLDRLEEAT"
     gene            complement(7110..7988)
                     /locus_tag="VFN42_13660"
     CDS             complement(7110..7988)
                     /locus_tag="VFN42_13660"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HET8997709.1"
                     /translation="MQIRPTGETLGARIDGIDLAEPLPDADRRLILHALGQYGVLCFP
                     RQSMTTGQLAGFGRRFGELEINVANAFHEPDHPEMMILSNMRQDGKPIGANDAGQGWH
                     TDMSYSKDIALANILHAQHVPHRHGKPLGETQFRNMHAAYDDLPDSMKQMLEGRTATH
                     DFAKFWDMMVAKPGSARKPLTAEQRAKRPPVSQPLFRRHPITGRFVLYANPGYTMRID
                     GMDQQESGEILDFLFRHQERAAFLHTHRWTEGDVLMWDNIGTVHNAVGDYLPDEPRYM
                     RRVQVMATLDYPSLVQ"
     gene            8087..8512
                     /locus_tag="VFN42_13665"
     CDS             8087..8512
                     /locus_tag="VFN42_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET8997710.1"
                     /translation="MQQDFAQRLVEGLPDAIVFADAQGVIRMWNAGAQRIFGFAAAEA
                     IGQSLDLIIPENLRARHWSGFAHTMQTGQSRYGAGDLLSVPALHKDGRRISVEFTIVP
                     FHGADGSIEGIAAVMRDVTARFEELRSLRRQLKETRQGG"
     gene            8823..>10181
                     /locus_tag="VFN42_13670"
     CDS             8823..>10181
                     /locus_tag="VFN42_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997711.1"
                     /translation="MTSSSPNLAEPSGEQVAPGGTVAVPGGYSDSFAQANPGMLYLDI
                     TDSSGLLSADDAGGNPVAGSGSSTIALSATYADVNAILGSLHYTAGGSSGSDSIQVQV
                     WNQAGTETTGAVPVTIAAGGGTTDRWNGTVSSDWNNAANWSTGAVPTSGDTVQIYGNN
                     PHDPTLSNATLTGETIDIAGSGNSTPLVTLNNVALNSLVDGSNGARVSIGGTLTIGSQ
                     GTLLASSNTFSLSGTAETIVNNGLIASQTNGDMLIYNTHTNNTAAATLTNNGSIAANG
                     GQINLLSDASFPGAPPHWTLLNSGSLTIGNNGGLALNGTVNGGQVAFNGAGTLELQQA
                     QALAGGATLTGFGQGDQIALASGLGTITAFNNGTLDVGTQNGGLTETIPLAGSYTYGN
                     FEQQADTYPNTTSQIVYAAGGSASGQLKTDIVSPASDTVAQGGTLSLVDVSIQTTGTS
                     TNT"
BASE COUNT         1697 a         3521 c         3454 g         1509 t
ORIGIN      
        1 ccgtccagca tttacgccac ggaggccagg gcgacgcagg aggagacgcg ggcgctgcat
       61 catctgccga acaatggcca tcagcggcgg atgcggcatc cgtccgcgct gctgccggcc
      121 ctcgcggcgc aggcggcggc gcacacgcac cgggcgagga accggctgcg gcggcggtga
      181 tgcgcggtgc gggcctgctc cgggccagcc gtccggctgt ccttgcttcc cgcgcgcgtc
      241 cggctacctt ccccgccgaa ccacccagga agcaccgtgc cctctgaact ccagcccccc
      301 cgtggcaccc acgacctgat cggcgaggag cagcgccgcc acgcccatgt ggtggacacc
      361 gcccgccgca tcgccgcgac ctacggcttc gacgaatggt ccaccccgat cttcgaggac
      421 acccgcgtgt tcgcccgcac gctgggcgag acctccgacg tggtcaccaa ggagatgtac
      481 agcttcgagg accgcggcgg cgacagcatc accctgcggc ccgaagccac cgccggcatc
      541 tgccgcgccc tggtgtccaa cggcctgacc cagtcgctgc cgcagaaggt gttctacact
      601 ggcccgatgt tccgctacga gcggccgcag aagggccgct accgccagtt ccaccagatc
      661 gacatcgagc tgatcggctc ggccgaaccg ctggccgatg ccgagatcat cgcctgcggt
      721 tggcacatcc tgcaggaact cggcgtggcc ggggaaacga tcctggagat caatacgctg
      781 ggcgacaagg cgtcccgcca ggcctatcgc gacgccctgg tcggttactt caacgaacac
      841 caggccagcc tgtcctccga cagcctgtcc aggctgcagc gcaatccgct gcgcatcctg
      901 gacagcaagg acgagaacga ccgccggatc gtcgccaatg cgccggtgat cgccggccac
      961 ctgaccgacg ccgccgcggc gttctatgcc gcggtgcgcc gctacctgga cgcgttcaag
     1021 gtgccgtacc gggagaatcc gcgcatcgtc cgcggcctgg actactacgg ccataccgcc
     1081 ttcgagttcg tcaccagcaa gctgggcgcc cagggcacgg tgatgggcgg cggccgctat
     1141 gacgggctgg tggaggagat gggcggcccg ccgaccccgg ccgtcggctt ggcggccggc
     1201 gtggagcggc tggccatgct gctgcaagac ccgccgccgc cgcccgcgcc ggtcgccgtg
     1261 gtgccggtcg gcgaggcggc cgaactggcc gcgctggatg cgctgcaaag cctgcgtgcc
     1321 gccggaatcc gctcggaaat ggcactgcgc ggcaatctcc gccgccgcat ggagcgtgcc
     1381 aaccgcatcg gcgcccgcgc cgcggtgatc atcggccagg acgacatcgc ccagggcgtc
     1441 gcccaggtga aggacctgcg caccggcagc caggaggcgg tggcgctggc cgcactgccc
     1501 gaccggctgc gatgaacctg acgcataagc tggaccgcat cgctgcccgc gccgacgagc
     1561 tgcaggcgat gctggcggaa ggcgccacgg gcgagaccta cgtgaaggcg tcgaaggagt
     1621 tctccgaact cgagccggtg gtggcccgta tcggcgagct gcgtgcggcg gagaaggcgc
     1681 aggcggaagc cgaggcgatg ctggccgacc cggaaatgcg cgaactggcc gaggtggagc
     1741 tgcacagcct gaagcagcgc attccggtgt tggagcacga gctgcagctg gcgctgctgc
     1801 cgaaggacga ggcggatgcg cgctccgcca tcctggagat ccgccccgcc gccggcggcg
     1861 acgaagccgc gctgttcgcc gcagagctat tcggcatgta ccagcgctac gccgaaagcc
     1921 ggggctggcg cttcgagatc ctggaataca acgacaccgg cctgggcggc ctgaaggaag
     1981 gcatcgccga gatcaacggc cgcggcgtgt tcgctcggct caaattcgaa tccggcgtgc
     2041 atcgcgtgca gcgcgtgccg gcgacggaga gccaggggcg catccacacc tccacggtga
     2101 cggtggcggt gctgccggaa gccgaggagg tggacgtgca gatcgacgag ggcgatctgc
     2161 ggatcgacgt ctatcgggcc tccggcgccg gcggccagca cgtcaacaag acggaaagcg
     2221 ccgtgcgcat cacccacctg ccgtccggca tcgtggtggc gatgcaggag gagaagagcc
     2281 agcacaagaa ccgcgccaag gcgatgacca tcctgcgcgc ccgtctctat gagcagcagc
     2341 gcgcggcggc gcatgccacc cgcgcggcgg accgcaaatc ccaggtcggt accggcgacc
     2401 gcagcgagcg catccgcacc tacaacttcc cgcagggacg ggtgtcggac caccgcatca
     2461 acctgacgct gtacaagatc gaccgggtga tgcagggcga gctggacgag ttcatcgatg
     2521 ccctgaccgc ggaagaccag gccgcccgcc tggccgcggc ggaagcgtga cggagaggga
     2581 caggcgggac cagactccgg actccccctc cccttgcggg agggggagtc tttcgagtct
     2641 gccaccccgg tctccgggaa cccacgaccg actcctcccc ccaaccttgc tccgcttggc
     2701 ggcccaagcc cacaagggga gggggagtcc cgtgaccaat ccccgccttc ggccccgccc
     2761 acccgcgccc agtgcattgc cgccgctgcc ggccggctgc gtgccgccgg caccgattcc
     2821 ccgcggctcg aagcgcggct gctgctcgcc cacatcctcg gcctgacgcc aaccgctttg
     2881 ctgcgcgacc cgagcacgcg cgtcccccca gaggcgcagg cgcgctttgc cgcgcttgtg
     2941 gaccgtcgcg ccggtcggga gccgctggcc tatatcctcg gccaccggga attctggagc
     3001 ctggatctgg ccgtctcgcc ggccacgctg atcccgcggc ccgactccga aaccctggtc
     3061 gccgccgccc gcagtgcctt cgccggacgc gcgccgcccc acagcatcct ggatcttggc
     3121 accggaaccg gcgccctcct gctcgccgcg ctgagcgaat tccccgcggc ctggggcgtc
     3181 ggagtcgatc gcgtgccggc cgccgcgcgc ctggcggcgg ccaatgccgc ggcgctggat
     3241 ctggcgccgc gcgcggtgtt cctggccggc atctgggcgg atgcgctggc cgcgcggttc
     3301 gacctggtct tgtgcaaccc gccttatatc cccacttcgg agctgcaggc gctgatgccc
     3361 gaagtggcac gccacgagcc cgcttcggca ctggatggcg gtgccgatgg gctggacgcc
     3421 taccgcttga tcattccgga gcttccccgg ctgctgtcgc cgcaaggcgt tgccgtgctg
     3481 gaactgggcg ccggacaggc ccaggcggta gccggactgg ccaggcaggc agggctggca
     3541 agcggaacgc aggacgattt ggcgggggtg ccgcgcgcat tattgctggc aaaccgctct
     3601 gccatgaaaa aaccgtttgg cacaacgaag tgggcagtct agcttgcagg catggcgggg
     3661 atcgccgcga agcggcggat tccggtcagt ttgaccgact cggtcgccat tgggcggccc
     3721 atgacgggca ggctgtgcgc cagcaccaac gtcgatcgat cgagacctcg ccgcttggtt
     3781 cacccaggga cgcgcagcaa cgggcttagc ccggcgcccc ggagctttgt ggcgcggacc
     3841 gacaacggga aaatgtgacg gcattagaat gaacatgaag cgtatgcgtg gtcgcggtca
     3901 tcgctccggt ggcggcggag gtggtggtgg cggcatgcgg caccacggcg gtgccggtgg
     3961 cggcggtatt ccgctcaatc ggaaccacgt gttcgacagc aacggcccgg atgtccgcat
     4021 ccgcggcacc gcccaacagc tttccgaaaa atacctccag ctcggccgcg acgcgaccag
     4081 tagcggtgac cgggtgatgg ccgaggccta tttccagcat gcggaacact acttccgcat
     4141 cctgaacgcc atgaaccagg cggcgcagca aggccagccg agcgggcagg gcggccagcg
     4201 ccggcactac cagggcaatg gcgaggacgg gcagtcgtcc ggcgggtcct ccgagtccga
     4261 ggagtccgac tcgcgcgccc ctggcctggg cgaccagccc gagtcgcggg aaatccccat
     4321 cgccgctgcg ccgcccgaag cctgagccag ccgggcagcg gaaccgccgg tcgccccttg
     4381 cgccggcgtt ctgaatcggc agagtccgcc ccgcccgatc aggagcctcg ccatggccga
     4441 ttccgccccg ctggtcaccg acccctttgc gcgcttccgc gcatggatgg ccgaggccga
     4501 ggccagcgaa ccgtccgatc ccaacgcgat gatcgtcgcc accgcgacac cggacggcca
     4561 tccgtccgcc cgcgcgatcc tgctgaaagg ggtcgatgac cgcggcttcg tgttctacac
     4621 caacaaggaa agccgcaagg cggacgagct ggccgccaac gcccagatcg ccctgctgtt
     4681 ccattggaag tccctggcgc ggcagatccg catcgagggc cgcgtggaac acgtcaccgg
     4741 cgccgaggcg gacgcctatt acgcgtcccg cccgcgcatc tcccgcctgg gcgcctgggc
     4801 gtcacggcaa tcccgtccgc tggacgaacg cgccacgctg gagcggcggc tggcggaata
     4861 tgaggcgaaa tatcccggcg aggacatccc ccggccggaa tactggtccg gctatcgcgt
     4921 gctgccggac cgcttcgagt tctggcagaa catgcccttc cgcctgcacg accgcacgac
     4981 ctacgtgcgg gatgcacagg gcggctggag catcggcaag ctgttcccct agcccggctc
     5041 agcccgaaga gggcggcagc ttgccgcggc ggagctgccg cacatccgcc gacagcacgt
     5101 tgattccacg gtcgcggaca accaactggt tcgaccggca atcgttcagg ttcggcgcct
     5161 tcagggtgcg gccgttgttc agcttcacca ccacgtccgc caggcactcc ttgcgcggca
     5221 ccacgatctg ccgtgcttcg ccgggctgga tcggcgaata ggtcagcgcc cggatcttgc
     5281 cgtcggtcat gcgtgcgcgg gcgaacacga ccggcgcgtg gctcatgttc ttcagcgaga
     5341 acgagcgcac gttctggccg gacggtccca gcggggcgga gggccgcacc tgggccaaag
     5401 cgggggtggc gaatagcagc agggcggcca ggagcgccgg tcgcggcatc gggttcgtcc
     5461 ttttgctgac cgcaagcgaa acgccgcacc gccgcgccgg ttgccgccgc gtcacgttgc
     5521 ctcctccagc cggtccagga aggatctggc ccaggcgcgc gccgtaccgc gctgcaccgt
     5581 ttcgtgcatc gagcgccacc gccgctggcg ttcttccagc ggcatcgaca gcgctgcatc
     5641 gaccacttcg gccagttcat ccggatcatg cggattgacc agcagcgcgc cgtccagctc
     5701 ctccgccgcg ccggcaaact gcgacagcac cagggcgccc gggttctccg ggtcctgcgc
     5761 cgccacaaac tccttcgcca ccaggttcat gccgtcacgc agcggcgtga ccagcccgac
     5821 ccgggccagc cgatggaagc cggccagcgt ggtgcgcgga atcgcccggg tgatgtagcg
     5881 caatggggtc cagtcgaact cggcgttctc gccattgatc cgcccggcca gctcgtccag
     5941 ttcccgccgc aacgcgcgat agcggccgat cgcgccgcgc gacaccggtg cgacctgcag
     6001 gaaggtcacc cggttgcggt gctgcgggaa acgccgcagc agcttggcat agccgcggaa
     6061 ccgctcggga atacccttgg tgtagtcaag ccggtccacc cccaggatca gcgtgcggtc
     6121 gcccaggctg ccatgcagcc gctccacctc gggacccgac gccgcgtgca ccgcggcatt
     6181 ggcgaagctg gccgcgtcga tgccgatggg gaacgcctcc accagcgcgc cgacctcggc
     6241 atcgcgcagc gcctgggcca gatacatggc gtctgccttg gtctgggtgc cgaccagctg
     6301 gtaggccccg aacgccttca gcagccgctg ggactgcggc agggtttcga acagcggtgc
     6361 cggcgggaag gggatatgca ggaagaaccc cagcctgatc cgttcgcagc gctctcgcag
     6421 catgcggccg agcggaatca ggtggtagtc gtgcacccac accagatcgc ccggccgcaa
     6481 cagcggggcc agcgcatcgg cgaactgttc gttcaccgcc atgtaggctt cgaagttctc
     6541 gcggctgaat tcggccaggc cggcgcgcca gtgcatcaac ggccacagcg tgccgttgga
     6601 gaacccctca taatagccgc cgaacagcga tgccgggata tcgatggtgg cctgggtcag
     6661 ccgccgtgcg tgggtgactt gcggcgcccc gggggcgcca tggcaggtgc ggccggacca
     6721 gccgaaccac aatgtctcct gtgccgcgac cgcgtccttc agggcgacgg ccagaccgcc
     6781 ggccgcctga ttgcgctccc gcggcgtggg cacgcggttg gatacgatca cgagccggga
     6841 caatgcctct ccttcccttc tgttcatacg gcgcccggtc tcgacgggat ctgccggcaa
     6901 agggagaggc tgcgctggcc cgccggagca gcctgatcgg tgcgcaatcg tcaccaagcc
     6961 gtccgtcagt gcgctggacg cggagcacgc ggcctccccg gcgcgggtga acttacccga
     7021 gccgggtggg tttggccatc ttccgttccc aatccacccc cgtgcggcag cgcgtaatga
     7081 ccgccgatca gcccttcgtg atcaggccgc tattgcacca gcgacggata atccagcgtc
     7141 gccatcacct ggacccggcg catatagcgc ggctcgtccg gcaggtagtc gcccaccgcg
     7201 ttgtgcaccg tgccgatatt gtcccacatc aggacgtcgc cctcggtcca gcgatgggtg
     7261 tggaggaacg cggcgcgttc ctggtggcgg aacaggaaat ccaggatttc cccgctttcc
     7321 tgctgatcca tgccgtcgat ccgcatggtg tagccggggt tggcgtacag cacgaagcgg
     7381 ccggtgatcg gatggcggcg gaacagcggc tgcgacaccg ggggcctctt cgcccgctgc
     7441 tctgctgtca gcggcttgcg ggcggatccc ggcttggcga ccatcatgtc ccagaacttc
     7501 gcgaagtcat gcgtcgcggt gcggccctcc agcatctgct tcatcgagtc cggcaggtcg
     7561 tcataggcgg cgtgcatgtt gcggaactgg gtctcgccca gcggcttgcc gtggcgatgc
     7621 ggcacgtgct gcgcgtgcag gatgttcgcc agcgcgatgt ccttggaata ggacatgtcc
     7681 gtgtgccagc cctgcccggc gtcgttcgcg ccgatcggct ttccgtcctg cctcatgttg
     7741 gacaggatca tcatttccgg atgatcgggt tcgtggaagg cgttggcgac gttgatctcc
     7801 agttcgccga agcggcggcc gaacccggcg agctggccgg tcgtcatgct ctggcgcggg
     7861 aagcacagca cgccatactg gcccagggcg tgcagaatga gccggcggtc cgcatccggc
     7921 agcggttcgg caaggtcgat gccatcgatc cgggcgccga gcgtttcacc ggttggcctg
     7981 atctgcatgg ggtgcctccg agacagcgaa cggcggccat ttcagcgcgg aaaccgggcg
     8041 ggggaaaggc cggccgccgc ccacggcgca gacagaggag tgagcgatgc aacaggattt
     8101 cgcgcaacgg ctggtcgaag gactgccgga tgccatcgtc tttgccgatg cccagggcgt
     8161 gatccgcatg tggaatgccg gtgcgcagcg catcttcggc ttcgccgcgg cggaagcgat
     8221 cggccagtcg ctcgacctga tcattccgga gaacctgcgc gctcggcatt ggtcgggttt
     8281 cgcgcacacg atgcagaccg ggcagtcccg ttacggcgcg ggcgatctgc tgtcggtgcc
     8341 cgcgctgcac aaggacggcc ggcgcatctc ggtggagttc accatcgtgc cgtttcacgg
     8401 cgccgacggc agcatcgaag gcatcgccgc ggtgatgcgt gacgtgacgg cgcgcttcga
     8461 ggaactgcgc agcttgcggc gccagctcaa ggaaacgcgc cagggcggct gacggagagt
     8521 tgagcgcgcg gcgcattgcc ggtgcgcaac gctcccggcg cgggtatttc gccagaggtc
     8581 aataaatggc gggcgccttg ccgagaggcg ttccggccga gagcggcatc atcggccaga
     8641 agtcccgact gtatcggccg tggatgatta ctggctgtca aaggttgcag tggggatggt
     8701 ggaatgtttc cccgtggttt tggcgtcacc acgtgtaatc gatttgcacc gctgcgctga
     8761 gagatgattc ctcccggcgg gacgtgaggc gcatgcaaca cgtcgaacag ggagaatggt
     8821 ccatgacgag cagcagcccg aatttggccg aaccgtcggg tgaacaggta gcgccgggtg
     8881 gcacggtggc cgtgccgggc ggctattccg acagctttgc ccaagcgaat cccggcatgc
     8941 tttacctgga catcaccgac agcagcgggc tgctgagcgc cgacgatgcc ggcggcaacc
     9001 cagttgccgg ttcgggcagc agcaccatcg ccctcagcgc cacctatgcc gacgtgaacg
     9061 cgatccttgg cagcctgcac tacacggcgg gcggcagcag cggctcggac agcatccagg
     9121 tccaggtctg gaaccaggcg gggacggaga ccaccggcgc ggtgccggtg acgatcgcgg
     9181 ccgggggcgg cacgacggat cgctggaacg gcaccgtcag cagcgattgg aacaatgcgg
     9241 ccaactggtc caccggcgcg gtgcccacca gcggcgacac cgtgcagatc tacggcaaca
     9301 acccgcatga cccgacgctg tcgaacgcga ccctgaccgg cgagacgatc gatatcgcag
     9361 gcagtggcaa cagcacaccg ctggtgacgc tgaacaatgt ggcgctgaat tcgctggtgg
     9421 acggcagcaa tggcgcccgt gtcagcatcg gcggtacgct gacgatcggt tcgcaaggca
     9481 cgttgctggc gagcagcaat acgttctctc tgtccggcac ggcagagacg atcgtgaaca
     9541 acgggctgat cgcctcccag accaacggcg atatgctgat ctacaacacc cacaccaaca
     9601 acacggccgc cgccacgctg accaacaatg gcagcatcgc ggccaatggc gggcagatca
     9661 acctgctctc cgatgcatcc tttccgggcg cgccgccaca ctggactctg ctgaattccg
     9721 gcagcctgac gatcggcaat aatggcggct tggcgctgaa cggcaccgtc aatggcgggc
     9781 aggtggcgtt caacggcgcc ggcacgctgg agttgcagca ggctcaggcg ctcgccggcg
     9841 gcgccacgct gaccggcttc ggccagggcg accagatcgc gctggcctcc ggtctgggca
     9901 ccatcaccgc gttcaacaac ggcaccctgg acgtcggcac ccagaacggc gggctgaccg
     9961 agacgattcc cctcgccggc agctacacat acgggaattt cgagcagcag gcggacacct
    10021 atccgaacac gacgtcgcag atcgtctatg cggccggcgg cagtgccagc ggacagctga
    10081 agaccgacat cgtctcgccg gccagcgata cggtagcaca gggcggcacg ctcagcctgg
    10141 tggacgtgtc gatccagacc acggggacca gcacgaacac c
//
LOCUS       DASSZH010000632         3378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1174664, whole genome shotgun sequence.
ACCESSION   DASSZH010000632 DASSZH010000000
VERSION     DASSZH010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174664"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2552)
                     /locus_tag="VFN42_13675"
     CDS             complement(<1..2552)
                     /locus_tag="VFN42_13675"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET8997712.1"
                     /translation="MNVSTPFIARPVATILLAIGLMLSGIVAHQFLPVAALPSVDIPT
                     IVVMAARPGADPETMANSVAAPLERRLGQIPGVSEITSTSSIGNSSVIVQFDIDRDIN
                     GAAHDVQAAINAAMTDLPDDLPTRPYYRKFNPADAPIMTIALSSNTRSTAQVYDAADT
                     VLMQRLSQVEGVGQVQENGAEKPAVRVQLDPVRLAAAGLSAQSVYGAIRAANVLQPTG
                     GFEGPRHAETIGVNGQMSQAEQYGRLVLKAGKGAVVTLSDVATVVNSTANTRVAAWDG
                     KQPAILLTITKEAGANVIETVDRIRALLPQLKSWMPAGINVTVVQDRTATIRASVEDV
                     QYTMLLTIGLVLLVVVIFMRRAIPTVAAAVTVPLSISGTLAGMWFCGFTIDNFSLMAL
                     TISVGFVVDDAIVMIENIVRHMERGLPPLRAAILGARQIGFTVVSISLSLVAVFIPLI
                     FMTGILGRLFHEFAMTLTLAILVSAVVSLTLTPMICGRWMHLDPPAATHGRVWRALDH
                     AFDATQRFYARTLDWALLHRGFMLLVMLLTIVLTVRLYTVVPKGFMPTQDTGLLIGST
                     LASPDVSFQTMEKLQREAVDVLLRDPAVATVSSTVGVSSGWSTMNRGHLTVTLKPVSQ
                     RHLSSEQVIARLRGPLEKIGGLQTVLFSAQDLRGGGRQGGSQFQFVVIAPSVSEMRHW
                     ALLMEEKLRQTPGILDVTSDQDRAGPQVNVMIDRDAAARLGVRAIDIDNALDNAYAQR
                     QISSIYTPRNQYKVVLEIDPHLQTDPSLLNRLYVASTTGKQVPLSAVAHFERSTAPLA
                     VRHQGQFPAATLSFNLPVGEALGTAEQAVERAASELRLPSDVHTEFAGNARW"
     gene            complement(2713..>3378)
                     /locus_tag="VFN42_13680"
     CDS             complement(2713..>3378)
                     /locus_tag="VFN42_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET8997713.1"
                     /translation="NLEPGYALGAAVNAVAAAERQIGMPATISGSYTGDAAEFQKSLA
                     AEPWLIGAAVVVIYLVLGVLYESWIHPVTILSTLPSAGIGALLALMACGIDLSLVALV
                     GIVLLMGIVKKNAIMMVDFAIEAERAGAVTPEAAMREACLLRFRPIMMTTMAALLGAL
                     PLVLERGAGSELRWPLGVTIIGGLLLSQFLTLYTTPAIYLAFERLRLRFAGPETPEPA
                     AAE"
BASE COUNT          594 a         1148 c         1112 g          524 t
ORIGIN      
        1 atccagcggg cattgccggc gaactcggtg tgcacgtcgg atggcaggcg cagttcgctg
       61 gcggcccgtt ccaccgcctg ttccgcagta cccagcgcct cgccgaccgg caggttgaag
      121 ctgagcgtcg cggcggggaa ctggccctgg tggcgcaccg ccagcggcgc ggtgctgcgc
      181 tcgaaatgcg caacggcgga cagcggcacc tgcttgccgg tggtgctggc cacgtacagc
      241 cggttcagca gcgacggatc ggtctgcagg tgcggatcga tctccagcac caccttgtat
      301 tgattgcgcg gcgtgtagat ggacgagatc tgccgctgcg cataggcatt gtccagcgcg
      361 ttgtcgatgt cgatcgcgcg cacgcccagc cgcgccgcgg catcgcggtc gatcatcacg
      421 ttcacctgcg gcccggcgcg gtcctggtcg gaggtgacgt cgaggatgcc cggcgtctgc
      481 cgcagcttct cctccatcag cagcgcccaa tgccgcatct cgctgacact cggcgcgatc
      541 accacgaact ggaactgcga accgccctgc cgcccgccac cgcgcaggtc ctgcgcggag
      601 aacagcacgg tctgcaagcc accaatcttc tccaatggcc cccgcagtcg ggcgataacc
      661 tgctcggagg acagatggcg ttgtgatacc ggcttcagcg tcacggtcag gtggccgcgg
      721 ttcatcgtgc tccagccgct ggagacaccg accgtggagc tgaccgtcgc cacggccggg
      781 tcgcgcagga gcacgtcaac tgcttcgcgc tgcagcttct ccattgtctg gaacgacaca
      841 tccggcgatg cgagcgtgga gccgatcagc aagccagtgt cctgggtggg catgaagccc
      901 ttcggtacca ccgtatagag ccgcaccgtc agtactatgg tgagcagcat caccagcagc
      961 atgaagccac gatgcagcag cgcccaatcc agcgtgcgcg cataaaaccg ctgggtcgcg
     1021 tcgaaggcat gatccagcgc gcgccagacg cggccatgcg tggcagcggg cgggtccagg
     1081 tgcatccagc ggccgcagat catcggcgtc agcgtcaatg acaccacggc ggagaccagg
     1141 atcgccaggg tcagcgtcat ggcgaattca tggaacagcc ggcccaggat gccggtcatg
     1201 aagatcagcg gaatgaacac cgcgaccaat gacaggctga tcgacaccac cgtgaagcca
     1261 atctgccgcg cccccaggat cgcagcccgc agcgggggca ggccccgctc catgtggcgg
     1321 acgatgttct caatcatcac gatcgcatcg tccaccacga agccgacgga gatcgtcagc
     1381 gccatcagtg agaagttgtc gatggtgaag ccgcagaacc acatgccggc cagcgtgccg
     1441 gaaattgaaa gcggcaccgt caccgctgcc gccaccgtcg ggatcgctcg gcgcatgaag
     1501 atgaccacca ccagcagcac caggccgatg gtcagcagca tggtgtactg cacgtcctcc
     1561 acgctggcgc ggatggtggc ggtgcgatcc tgcaccacgg tgacattgat accggctggc
     1621 atccaggact tgagctgcgg cagcagggcg cggattcgat caaccgtctc gatgacgttg
     1681 gcgccggcct ccttcgtgat ggtcagcagg attgcgggct gcttgccgtc ccaggcggcg
     1741 acccgggtgt tggccgtgct gttgaccacc gtcgccacgt cggacagcgt caccacggcg
     1801 cccttgccgg ccttcagcac caggcggccg tattgttcgg cctgcgacat ctggccgttt
     1861 acgccgatgg tctcggcgtg acgcgggcct tcgaagccgc cggtcggttg caggacattg
     1921 gccgcgcgga tcgcgccata cacgctctgc gccgagagac ccgccgcggc gagccgcacc
     1981 ggatccagct gcacacgcac ggccggcttt tccgcgccgt tctcctgcac ctggccgacg
     2041 ccctccacct gcgacaggcg ctgcatcagc acggtgtccg cggcatcata gacctgcgcg
     2101 gtgctgcgcg tgttggacga caacgcgatc gtcatgatcg gcgcatcggc cgggttgaac
     2161 ttgcgatagt acggccgcgt cggcaggtcg tcaggcagat cggtcatggc cgcgttgatg
     2221 gccgcctgca cgtcatgcgc cgcaccgttg atgtcacggt cgatgtcgaa ctgcacgatg
     2281 accgagctgt tgccgatgga tgaggtggac gtgatctcgc tgacacccgg gatctggcca
     2341 aggcgacgct ccagcggcgc ggcgacggag ttcgccatgg tctccggatc ggcgcccgga
     2401 cgcgcggcca tcaccacgat ggtggggata tccaccgacg gcagagcggc aaccggcagg
     2461 aactggtggg cgacgatgcc ggacagcatc agcccgatgg ccagaaggat ggtggcgacc
     2521 ggccgcgcga tgaatggggt ggagacgttc atcgccgcca gtcaccgcaa gaacgccccc
     2581 tccccttatg ggagggtagc ggggagaggg ggcgaacgtg agttcccggg gaccaatcgc
     2641 acgacccctc cccccagccc cgtcccgcaa gggaaggggg agtcgtgttc ggccgccctg
     2701 cctgtgcccg gttcattccg ccgccgccgg ttccggcgtc tccgggccgg cgaaccgcag
     2761 gcgcaggcgt tcgaacgcaa ggtagatcgc cggcgtggtg tacagcgtga ggaactgcga
     2821 cagcagcagc ccgccgatga tggtcacccc cagcggccag cgcagctcgc tgcccgcacc
     2881 gcgctccagc accagcggca gggcgccgag cagcgcggcc atggtggtca tcatgatggg
     2941 gcggaagcgc agcaggcagg cttcgcgcat cgcggcttcg ggcgtaaccg cgccggcccg
     3001 ttccgcctcg atggcgaaat ccaccatcat gatggcgttc ttcttcacga tccccatcag
     3061 cagcacgatg ccgaccagcg ctaccagcga cagatcgatg ccgcacgcca tcagcgccag
     3121 cagcgcaccg atgccggcgg aaggcagcgt ggacaggatc gtcaccgggt ggatccagct
     3181 ctcgtacagc acgcccagca cgaggtagat caccaccacg gcggcgccga tcagccaggg
     3241 ttccgccgcc agcgatttct ggaattccgc agcatcaccg gtgtagctgc cgctgatcgt
     3301 cgccggcatg cctatctgcc gctccgcggc agcaacggca ttcaccgcgg cacccagcgc
     3361 gtatcccggc tccaggtt
//
LOCUS       DASSZH010000633         2903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_918425, whole genome shotgun sequence.
ACCESSION   DASSZH010000633 DASSZH010000000
VERSION     DASSZH010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918425"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VFN42_13685"
     CDS             <1..401
                     /locus_tag="VFN42_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997714.1"
                     /translation="QIVAAIEIVENRYGDLVELGTPTLIADQVYHAAAVVGDAGTQDW
                     RNLDLLTLPGRIVVDGHIRDQGVGADLMGHPLNSLAWLAGSPVAAAFGGLRAGQVIML
                     GSVTPPVWLTGPATITVEFAPLPPVQLRLA"
     gene            776..1990
                     /locus_tag="VFN42_13690"
     CDS             776..1990
                     /locus_tag="VFN42_13690"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET8997715.1"
                     /translation="MTKPAPRTGPLVGLKVLEIGHYIAGPFCTRILADLGAEVIKIEP
                     PGGDPFRGWGSAVNGHSVWFSIHGRNKLSVTLDLKRQRDAALRLAARADVLVENLRAG
                     QLERLALGPAELHAVNPRLVIARISGYGQDGPYREKPAFGAIGEAMGGLRHLTGYPPG
                     TTDLPPPRCGISISDDLAGMYAAIGLLSALWQRDAQGDGRQGTGRGRIIDVNLVDSVF
                     SLMEGMLPEYAMDGRIRQPVGAAIPTAAPTNTYPCADGKWLCIAGNSDLIFARLMQAI
                     GRPELAAEPAYQTNAGRCAQREALDAAISAWTRTLPAQQAEAVLDAAEVPCSRLFDIA
                     DCAADPHFRARAAVQDVDDPLIGRTLHPGPAIRLNGDAPEAVVAWPGPAAGAHTRYVL
                     EELLGEGGAPIS"
     gene            2084..2386
                     /locus_tag="VFN42_13695"
     CDS             2084..2386
                     /locus_tag="VFN42_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HET8997716.1"
                     /translation="MSFGTLGVGSLPVAMSPNPIARSRPPHLEFSSHARFRMEEPDID
                     RNSIESLLTAPQADISIDRSSGNYVFRFGDYRIIVKQAADNAFIVTSIFKTDQWPN"
     gene            2393..>2903
                     /locus_tag="VFN42_13700"
     CDS             2393..>2903
                     /locus_tag="VFN42_13700"
                     /inference="COORDINATES: protein motif:HMM:NF025448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shedu anti-phage system protein SduA
                     domain-containing protein"
                     /protein_id="HET8997717.1"
                     /translation="MSVLLPAPHFELSQAESQLLQFRAWLNETPYAIEQVATKRLRSL
                     PALCPLLGMYDGRKYPTVQKWEFEIQGVFRADFIAGNPARKEFVLVEFEGAEQHSIFG
                     PSGTNQMRNWGDQIEHGFSQIADWTWAKNESQHSRIFQAALGADESVESYVLICGRDS
                     SLIDHERSRL"
BASE COUNT          474 a          984 c          988 g          457 t
ORIGIN      
        1 gccagatcgt cgccgcaatc gagatcgtgg agaaccgcta cggcgatctg gtggagctgg
       61 gcacgcccac gctgatcgcc gatcaggtct accacgccgc cgcggtggtc ggtgacgccg
      121 gcacccagga ctggcgcaac ctggatctgc tgacgctgcc tggccgcatc gtggtggacg
      181 ggcatatccg cgaccagggc gtcggcgccg acctgatggg gcatccgctg aactcgctcg
      241 cctggctggc cggctcgccg gtcgcggcgg cgttcggcgg gctgcgcgcc gggcaggtga
      301 tcatgctggg cagcgtcacc ccgccggtct ggctgaccgg cccggccacg atcaccgtgg
      361 agttcgcccc gctgccgccg gtgcagctcc ggctggcctg agcggtgccc gcaaaaagaa
      421 ggggcggcgc ggcttcggac cgcaccgccc aaggtggtcg cgcgatgggc gatccagcgc
      481 gacatcgggg aggaaacagg acaccggcga accaccggct ccgcattcgg caatatgggg
      541 gcatgaccct tatgaaggca taacgatccc gtgttgtttg cccgccgcag cccggatttc
      601 cgatcacata cggtcgaacg gagcaccgaa tgacgccggg taaggacgct tcccccggcg
      661 ggaggggcgg ttggcccagt cccgggggaa ctcatgcggc cccctcccgc cagggcggag
      721 cctgtctcgc ctgccccccg cccccagccc cggccacgat attgaggagc ccgccatgac
      781 caagcccgcc ccgcgcaccg gcccgctggt tggcctgaag gtgctggaga tcggccacta
      841 catcgccggc ccgttctgca cccgcatcct ggccgacctc ggcgccgagg tgatcaagat
      901 cgagccgccc ggcggcgacc cgttccgcgg ctggggatcc gcggtgaacg gccactccgt
      961 gtggttcagc atccacggcc gcaacaagct gtccgtgacc ctggacctga agcgccagcg
     1021 cgacgccgcc ctgcgcctgg ccgcccgcgc cgacgtgctg gtggagaacc tgcgcgccgg
     1081 ccagctggag cgcctggcgc tcggccctgc cgaactgcac gcggtgaacc cgcgcctggt
     1141 gatcgcccgc atctccggct acggccagga cgggccatac cgcgaaaaac ccgccttcgg
     1201 cgcgatcggc gaggcgatgg gcgggctgcg ccacctgacc ggctatccgc ccggcaccac
     1261 cgacctgccg ccgccgcgct gcggcatctc catcagcgac gacctggccg gcatgtacgc
     1321 cgccatcggc ctgctgtccg cgctgtggca gcgcgacgcg cagggcgacg ggcgccaggg
     1381 gacaggacgc ggacggatca tcgacgtgaa cctggtggac agcgtgttca gcctgatgga
     1441 gggcatgctg ccggaatacg ccatggacgg ccgcatccgg cagccggtgg gcgccgccat
     1501 cccgaccgcc gcgccgacca acacctatcc ctgcgccgac ggcaaatggc tgtgcatcgc
     1561 cggcaattcc gacctgatct ttgctcgcct gatgcaggcg atcggccggc cggagctggc
     1621 ggccgaaccg gcctaccaga ccaatgccgg ccgctgcgcg cagcgggagg cgctggacgc
     1681 ggcgatctcc gcctggaccc gcacgctgcc ggcgcagcag gccgaggcgg tgctggacgc
     1741 ggcggaggtg ccgtgttccc gcctgttcga catcgccgac tgcgcggcgg acccgcattt
     1801 ccgggcgcgc gcggcggtgc aggatgtgga tgacccgctg atcggccgca cgctgcatcc
     1861 tggtccggcg atccggctga atggcgacgc gccggaggcg gtggtggcgt ggccagggcc
     1921 ggcggcgggg gcgcacacgc ggtatgtgct ggaggagttg ctgggggagg ggggcgcgcc
     1981 catctcgtaa gcgtgcggct gatacggccc tggcggcact ccaaggttac gccgccggac
     2041 agaggctgct ggccttggtc cgtcatcggc cgtagactac gctttgagct ttggtaccct
     2101 tggcgttggg agtctgcccg tggctatgtc gccaaacccg attgcgcgca gccgtccgcc
     2161 gcatttggaa ttctcctcgc atgcccggtt caggatggag gagcccgata tcgaccggaa
     2221 ttccattgaa agtctgctga cggcgccgca ggcggacatc agcatcgacc gcagttccgg
     2281 caattacgtg ttccgctttg gggattaccg aatcatcgtc aagcaggcgg cggacaacgc
     2341 gttcattgtt acctccatat tcaagacgga ccagtggccg aactgagctg tcatgtcggt
     2401 cttgctcccc gcaccgcact tcgaattgag ccaggccgaa agtcagcttc tacagttcag
     2461 agcgtggctc aacgagactc cctatgccat cgaacaggtg gcgacgaagc ggcttaggtc
     2521 gctgcccgcc ttgtgcccgc tgctgggcat gtatgacggc aggaagtacc ccacggtgca
     2581 gaagtgggaa ttcgaaatcc aaggcgtgtt tcgcgccgat ttcatcgccg gcaatccagc
     2641 gagaaaggag tttgtgctgg tcgaattcga aggagccgag cagcacagca tttttggccc
     2701 ctccggcacc aaccagatgc gcaactgggg cgaccagatc gagcatggct tcagccagat
     2761 agccgactgg acatgggcca aaaacgaatc ccagcattca cgtattttcc aggcagcgct
     2821 cggcgccgat gagtcggttg agtcgtacgt gctgatttgt ggccgcgaca gttcgctgat
     2881 cgatcacgag cgatcgagac tgc
//
LOCUS       DASSZH010000634         3039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_407471, whole genome shotgun sequence.
ACCESSION   DASSZH010000634 DASSZH010000000
VERSION     DASSZH010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407471"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..321)
                     /locus_tag="VFN42_13705"
     CDS             complement(13..321)
                     /locus_tag="VFN42_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997718.1"
                     /translation="MTASRAYSTAGLLLSLAAGLAIMPRSMAGQTAPVLVTTDTPQYC
                     LHLLDQVSELMRLAPSAPPEVSSLSTEGQRMCGQGQTRGGIMRLRRALVLLKQQNLPP
                     "
     gene            complement(382..1827)
                     /locus_tag="VFN42_13710"
     CDS             complement(382..1827)
                     /locus_tag="VFN42_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET8997719.1"
                     /translation="MTESPAIAAQPGIAGSPAPGTPQRGFTMFRSAGFRFAVVYALLL
                     AASAAALAMFLWWSTAGLLDRQTEAAIRADAQGLAERWQEGGLPALVLTIDERLSGNV
                     DDDALYLLANARMQRIAGNLDHWPAVVRTNSTWYELPVDRAGTLSLALVWDYDLPGGY
                     HLLIGRDVQVRAQLRRLLTDALLWALVIVAAMAIAGALVVRSLLRRTLANVSSTANAI
                     AAGDFAQRVKLSGRGDEFDQLAEVINDMLDRIGRLMDGVRQVSNAIAHDLRTPITRAR
                     TRLEEASLRADSPEALRAAIDRATADLDGIVAVFQALLRISEIEAGSRRSAFTSLDLA
                     PALADLAELYEAVAEEKGVALTADIPERLPAYGDRELIQQAVANLLDNAVKFSPPGGT
                     VRLRAAAVPGSVEVAVVDEGPGIPAEDLKRATERFFRGESARSTPGAGLGLALVQAVA
                     QLHAGTLRLEDAHPGLAASLTLPTVDEPVHS"
     gene            complement(1824..2507)
                     /locus_tag="VFN42_13715"
     CDS             complement(1824..2507)
                     /locus_tag="VFN42_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8997720.1"
                     /translation="MRILVVEDDKDVAGFVVRGLREAGHVVEHADNGRDGLFMAASES
                     FDAVILDRMLPGGIDGLKILETLRGQKNSVPVLILSALAEVDERVKGLKAGGDDYLTK
                     PFAFAELLARVEALARRGKTEGPVTKLVVEDLELDLLSRQVKRAGQKIDLQPREFRLL
                     EYLMRHAGQVVTRTMLLEGVWDYHFDPQTNVIDVHVSRLRQKVDKPFPVALIQTVRNA
                     GYMLRAERA"
     gene            complement(2533..>3039)
                     /locus_tag="VFN42_13720"
     CDS             complement(2533..>3039)
                     /locus_tag="VFN42_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET8997721.1"
                     /translation="SAEGRDLGSSPFDSFLQLDAPINPGNSGGPIFDMNGEVIGVSTA
                     IVSPSGGSVGIGFAIPSDTVRRTVAELLAHGTIARGWLGVAIEDSTAGVRIASIEPGG
                     PAAHSGLRPGDVVVAIDGERINSSRGLIRAVAATPPGGTARLTVHRDGHVVDLPVTVG
                     RRPPESSG"
BASE COUNT          476 a         1080 c         1035 g          448 t
ORIGIN      
        1 aattcctctc gctcacggcg gcaggttctg ctgcttcagc agcaccagcg cccggcgcag
       61 gcgcatgatg ccgccgcggg tctggccctg gccgcacatg cgctgaccct cggtggacag
      121 gctgctcacc tccggcggcg ccgagggcgc caggcgcatc agctcgctca cctggtcaag
      181 caggtgcagg cagtattgcg gggtatcggt ggtcaccagc acgggggcgg tctggcccgc
      241 catcgagcga ggcatgatcg ccaggccggc cgccagcgaa agcagcaggc ccgccgtgct
      301 gtaggcgcgc gacgcggtca tcatagggca cttatcacag acacggcctc tccctcccgt
      361 gttgcgcacg tgacaagcat gttagctgtg taccggttcg tccaccgtcg gcagagtcag
      421 gctggccgcc aggcccggat gggcatcctc caggcggagg gtgccggcat gcaactgggc
      481 gaccgcctgg accagggcga ggccaagtcc tgcccccggc gtgctgcggg cggattcgcc
      541 gcggaagaag cgctcggtcg cccgcttgag gtcctccgcc ggaatccctg ggccttcatc
      601 gaccaccgcg acctccacgc tgcccggcac ggccgccgca cgcagccgca ccgtgccgcc
      661 gggcggggag aatttcaccg cattgtccag cagattggcc accgcctgct ggatcagctc
      721 gcgatcgcca taggccggca ggcgttcggg gatatccgcc gtcagtgcga cgcccttctc
      781 ctccgccacg gcttcataca gctccgccag gtcggccaag gccggcgcca ggtccagcga
      841 ggtgaaggcg gagcggcggg atccggcttc gatctcggaa atccgcagca gggcctggaa
      901 caccgcgacg atgccgtcca gatccgcggt cgcccggtcg attgccgcgc gcagggcttc
      961 cgggctgtcc gcgcgcaggc tcgcctcctc caaccgggtg cgggcgcggg tgatcggggt
     1021 gcgcaggtcg tgcgcgatgg cgttggacac ctgccgcacg ccatccatca gccggccaat
     1081 gcggtccagc atgtcgttga tgacctcggc gagctggtcg aactcgtcgc cgcggccgga
     1141 cagcttcacc cgctgggcga aatcgccggc ggcgatggca ttggcggtgc tggagacgtt
     1201 ggccagggtc cgccgcagca ggctgcgcac caccagggcg ccggcgatgg ccatcgcggc
     1261 gacgatcacc agggcccaca gcagcgcgtc ggtcagcaac cggcgcaact gggcgcgcac
     1321 ctgcacgtcg cggccgatca gcaggtggta gccacccggc aggtcgtagt cccacaccag
     1381 ggccagcgac agcgtgccgg cccgatccac cggaagctcg taccacgtgc tgttggtgcg
     1441 cacgaccgcc ggccagtgat ccagattccc ggcgatgcgt tgcatccggg cattggccag
     1501 caggtagagc gcgtcgtcat cgacattgcc gctcagccgc tcgtcgatgg tcagcaccag
     1561 ggccggcagg ccgccttcct gccagcgttc ggccaggccc tgggcatcgg cgcggatcgc
     1621 cgcctcggtc tgccggtcga gcaggccggc ggtggaccac cacaggaaca tcgccagggc
     1681 ggcggcactg gccgcgagca gcaaggcata gacgacggcg aagcggaagc cggcagagcg
     1741 gaacatggtg aagccccgct gcggcgtgcc aggggcgggg ctaccggcaa tcccgggttg
     1801 cgcggcgatc gccggcgact ccgtcacgcc cgctcggcgc gcaacatata gccggcgttg
     1861 cgcactgtct ggatcagcgc caccgggaac ggcttgtcca ccttctgtcg cagccgggag
     1921 acgtgcacgt caatcacgtt ggtctgcggg tcgaaatggt agtcccacac gccttccagc
     1981 agcatggtgc gggtgaccac ctggccggcg tgacgcatca gatattccag caggcggaat
     2041 tcgcgcggct gcaaatcgat cttctggccg gcgcgcttca cctggcggga cagcaggtcc
     2101 agctccaggt cctccaccac cagtttggtg accgggccct cggtcttgcc gcggcgtgcc
     2161 agcgcttcca cccgcgccag cagttccgcg aaggcgaagg gcttggtcag gtagtcgtcg
     2221 ccgcctgcct tcagcccctt cacccgctcg tccacctcgg ccagcgccga caggatcagc
     2281 acgggcacgc tgttcttctg cccgcgcagg gtttccagga ttttcagccc gtcgatgccg
     2341 ccgggcagca tgcggtccag gatcaccgcg tcgaagcttt cgcttgccgc catgaacaga
     2401 ccgtcgcggc cattatcggc gtgctccacc acgtggccgg cttcgcgcag gccgcgcacc
     2461 acgaagccag ccacgtcctt gtcgtcttcg accaccagga tacgcattcc aatcctccgt
     2521 tcgggcccgg actcagccgg acgattcggg cggacgccgg ccgacggtca ccggcaggtc
     2581 cacgacatgc ccgtcgcgat gtaccgtcag gcgcgcggtg ccgccgggag gcgttgcggc
     2641 aaccgcgcgg atcagcccgc gcgagctatt gatccgctcg ccatcaattg caaccaccac
     2701 gtcgccgggc cgcagcccgg aatgcgccgc cggaccgccg ggttcaatcg aggcgatgcg
     2761 cacgccggcc gtactgtcct cgatggcgac gcccagccag ccgcgcgcga tcgtgccgtg
     2821 ggccagcaac tcggcgacgg tacggcgcac ggtatcggac ggaatggcga agccgatgcc
     2881 caccgagcca ccggaaggcg agacgatcgc ggtggagacg ccgatcacct cgccgttcat
     2941 gtcgaagatc gggccgccgg aattgcccgg attgatcggg gcatcgagct gcaggaagct
     3001 gtcgaacggg ctggagccca ggtcgcggcc ctcggcgga
//
LOCUS       DASSZH010000635         4096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_577834, whole genome shotgun sequence.
ACCESSION   DASSZH010000635 DASSZH010000000
VERSION     DASSZH010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577834"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..1965
                     /gene="ilvD"
                     /locus_tag="VFN42_13725"
     CDS             103..1965
                     /gene="ilvD"
                     /locus_tag="VFN42_13725"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET8997722.1"
                     /translation="MPAYRSRTTTHGRNMAGARSLWRATGMQDGDFGKPIIAVANSFT
                     QFVPGHVHLKDLGQLVAREIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     AVEYMVNAHCADALVCISNCDKITPGMLMAAMRLNIPTVFVSGGPMEAGKIVKGGKTQ
                     RADLISAMVNAADPSITEADVAVQERSACPTCGSCSGMFTANSMNCLTEALGLALPGN
                     GTVVATHADRRKLFTEAGWLIVDLARRWYEQDDATALPRGIATFRAFENAMSLDIAMG
                     GSTNTVLHLLAAAQEGEVPFSMADIDRLSRRIPHLCKVSPSVANVHVEDVHRAGGVIG
                     ILGELDRAGLIHRDVPTVHTATLGDALARWDVRAEGREASDTASNFYLAAPGGVRTTE
                     AFSQNEHYDSLDLDRSAGVIRDPAHAFSADGGLAVCYGNIALDGAIVKTAGVDAALLK
                     FSGPARIFESQEAAVDGILGDKVKPGDVVVIRYEGPKGGPGMQEMLYPTSYLKSKHLA
                     TQCALITDGRFSGGSSGLSLGHISPEAAEGGAIGLLEEGDRIEIDIPNRALNVALSDE
                     ELAARRARMESRGNDAWKPQRNRVVSTALQAYAALTTSAARGAVRDVSQLARAR"
     gene            2128..3279
                     /locus_tag="VFN42_13730"
     CDS             2128..3279
                     /locus_tag="VFN42_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrpF domain-containing protein"
                     /protein_id="HET8997723.1"
                     /translation="MPQLALPAVFMRGGTSRAVMFRAQDLPERTRWDPIFLAAMGSPD
                     PNGRQLNGMGGGVSSLSKVCVLAPSDREDADVDYTFAQVQIREAVVDYRANCGNMSSA
                     VGPFAVDEGIVRPNGDTAVVRIFNTNTQRIIRSSFPLEDGRAAVDGDLAIPGVAGTGA
                     PVRLDFLAPGGATTGRLLPTGHAVDRMELPGIGPIDVSMVDAANACVFVRARDLGLHG
                     NELPDELERNAALLDRLQVIRRAGSVAMGIAADDAAARAINAVPFIGFIAPAMDAPTL
                     AGAALAADQVDLTVRFLSNGQPHRALPLTASLCTAIAARIGGTVVNEALRPGAADSIR
                     LGMPSGILTVGADVTQDASGAWVAHSGAFYRTARRLFDGRIYVPQPSAA"
     gene            complement(3377..>4096)
                     /locus_tag="VFN42_13735"
     CDS             complement(3377..>4096)
                     /locus_tag="VFN42_13735"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8997724.1"
                     /translation="VAALFMKDPPPGWRPAGWVPDEAKRRPTEHQFTLKEALGTWQMW
                     VLIASFILISSAGLAGVSKIVKYSNSFGFHAAAATAAAGGIAISNGLGRLALGWISER
                     IGRETAMIWSFILTGIFLFLTLGAGAIHSEFLFVLTAILAIFFWGPLFSLFPAVVGQY
                     FGDLASGSNYGVLYAIAKGSGGLYGGVLSAVLITDNGFPFAIGVAALMAICAGLLIIP
                     LKNHQPVAVSAGGLSTGGRTA"
BASE COUNT          666 a         1414 c         1393 g          623 t
ORIGIN      
        1 cgcgggctcg tatgagccgc gcttgccggc cggccactgt tccccggtgg cattttccgc
       61 taccagccct ggcacacgcc caacgctgcc aggacccttg ccatgcccgc ttaccgctcc
      121 cgcaccacca cccacggccg caacatggcg ggcgcacgca gcctgtggcg ggccaccggc
      181 atgcaggatg gcgatttcgg caagccgatc atcgccgtcg ccaacagctt tacccagttc
      241 gtccccggcc atgtccacct gaaggacctc ggccagttgg tggcccgcga gatcgaggcg
      301 gccggcggcg tcgccaagga gttcaacacc atcgcggttg atgacggcat cgccatggga
      361 catggcggca tgctgtacag cctgccgtcg cgcgagctga tcgccgatgc cgtggaatac
      421 atggtcaatg cccactgcgc cgacgcgctg gtctgcatca gcaactgcga caagatcacc
      481 cccggcatgc tgatggccgc gatgcggctg aacatcccta ctgtgttcgt ctccggtggg
      541 ccgatggagg ccggcaagat tgtgaagggt ggcaagacac agcgcgccga tctgatttcc
      601 gccatggtca acgccgccga ccccagcatc accgaggcgg acgtcgcggt gcaggaacgc
      661 tccgcctgcc cgacctgcgg ctcctgctcc ggcatgttca ccgccaattc gatgaactgt
      721 ctgaccgagg ccctcggcct cgctttgccc ggcaacggca ccgtggtcgc cacacatgcc
      781 gaccggcgga aactgttcac cgaagccggc tggctgatcg tggacctggc tcgccgctgg
      841 tacgagcagg acgatgccac ggcgctgccg cgcggcatcg ccacgttccg cgcgttcgag
      901 aacgccatgt cgctggatat tgcgatgggt ggttccacca acaccgtgct gcacctgctg
      961 gccgcggcgc aggagggcga ggtgccgttc tccatggcgg atatcgaccg gctgtcccgc
     1021 cgcatcccgc atctgtgcaa ggtgtcgccc tcagtcgcga atgtgcatgt cgaggacgtg
     1081 caccgcgccg gcggagtcat cggcatcctg ggcgagctgg atcgcgccgg gctgatccac
     1141 cgcgacgtgc cgacagtgca caccgcgaca ctgggcgacg ccctggcgcg ctgggacgtg
     1201 cgggccgaag gccgcgaagc cagtgacacc gcgtccaact tctacctggc tgcgccgggc
     1261 ggcgtacgca ccaccgaggc gttcagccag aacgagcatt acgactcgct ggacctggac
     1321 cgtagcgccg gggtgatccg cgatccggcg catgccttca gcgccgatgg cggactggcg
     1381 gtgtgctacg gcaacatcgc gctcgacggc gcgatcgtga agaccgccgg cgtggatgcc
     1441 gcgctgctga aattctccgg tccggcgcgg atcttcgaga gccaggaagc ggcggtggac
     1501 ggcatcctcg gagacaaggt gaagcctggc gacgtggtgg tgatccgcta cgaaggcccg
     1561 aagggcggcc ccggcatgca ggagatgctg taccccacca gctacctgaa gtcgaaacac
     1621 ctggcgacgc agtgcgccct gatcaccgac ggccgcttct ccggtggcag ttccgggctg
     1681 tcgctgggtc atatctcgcc ggaagcggcg gaaggcggcg cgatcgggct gctggaggaa
     1741 ggcgaccgga tcgagatcga cattcccaac cgcgcgctga atgtcgcgct gtccgacgag
     1801 gaactcgccg cccgccgcgc gcgcatggaa tcccggggca atgatgcctg gaagccgcag
     1861 cgcaatcgcg tggtgtccac ggcgctgcag gcctatgcgg cactgaccac cagcgccgcc
     1921 cgtggcgcgg tgcgcgacgt gtcacagctg gcgcgcgcgc gctgagaggg ctggcggccc
     1981 gaacagttat tccccctccc cttgtgggag ggggaggagt ctctctctct ctctctcccg
     2041 gccctaacca ccgcagcgtc ctccctggct tgacctccgc ctccctcgac gctgatcctg
     2101 cctgccgcaa acgcaggagg aaccgccatg ccgcagcttg cgctacccgc cgtgttcatg
     2161 cgtggcggca ccagccgggc ggtgatgttc cgcgcgcagg acctgccgga gcgcacccgc
     2221 tgggatccga tcttcctcgc cgcgatgggc agccctgatc cgaacgggcg gcagctgaac
     2281 ggcatgggcg gcggcgtatc gtcgctgtca aaggtctgcg ttctggcgcc gtcggaccgc
     2341 gaggatgccg acgtggacta cacctttgcc caggtgcaga tccgcgaggc ggtggtggac
     2401 taccgcgcca actgcggcaa catgagttct gcggtcggcc cctttgcggt ggacgagggc
     2461 atcgttcgcc cgaacggcga taccgcggtg gtgcgcatct tcaacaccaa cacgcagcgc
     2521 atcatccgtt ccagcttccc gctggaggac ggccgcgcgg cggtggacgg cgatctggcg
     2581 attccgggcg tggcgggtac cggcgctccg gtacggctgg acttcctggc gccgggcggt
     2641 gccacgaccg gccggctgct gcccaccggc catgccgtgg accgcatgga actgcccggc
     2701 atcggaccga tcgatgtctc catggtggat gcggccaatg cctgcgtgtt cgtgcgcgcc
     2761 cgcgatctgg ggctgcacgg caatgagttg ccggacgaac tggaacgcaa cgccgcactg
     2821 ctggaccggc tgcaggtgat ccgccgagcc ggctcggtcg ccatgggaat cgccgcagac
     2881 gacgcggcgg cgcgcgcgat caacgcggtg ccgttcatcg gcttcatcgc accggcaatg
     2941 gacgcgccga cgctggccgg cgcggcgctc gccgcggacc aggtggatct gaccgtgcgt
     3001 ttcctgtcca acggccagcc gcaccgggcg ctgccgctga ccgcatcgct gtgcaccgcg
     3061 atcgcggcac gcatcggcgg cacggtggtg aacgaggcgc tgcgccccgg cgccgccgac
     3121 agcatccggc tgggcatgcc gtccggcata ctgacggttg gcgccgacgt gacgcaggat
     3181 gcgtccggcg cctgggtggc gcatagcggc gcgttctacc gcacggcgcg ccggctgttc
     3241 gacggccgga tctacgtgcc gcaaccctct gccgcgtagc gccgcgtcgg caaaaaaagg
     3301 ccggcccgag ggccggccat cagtgcggag cgccggacgc gctaacggcg gccggtcggg
     3361 ccgccgttac gtggttctat gccgtgcgcc cgccggtcga gaggccgccg gccgataccg
     3421 cgaccggctg gtggttcttc agcgggatga tcagcaggcc cgcacagatc gccatcagtg
     3481 cggcgacgcc aatggcgaag gggaaaccgt tgtcggtgat cagcactgcc gacaacacgc
     3541 cgccgtaaag gccgccggag cctttggcga tggcatacag caccccgtag ttcgagcctg
     3601 acgccaggtc gccgaaatac tggcccacca ccgccgggaa caacgagaac agcgggcccc
     3661 agaagaagat cgccaggatg gcggtcagca cgaacaggaa ctcgctgtgg atcgcgccgg
     3721 cgcccagtgt caggaacagg aaaatgccgg tgaggatgaa gctccagatc atcgcggtct
     3781 cgcggccgat acgctcggaa atccagccca gcgccagccg gccaaggccg ttgctgatgg
     3841 caatgccgcc agccgctgcg gtcgccgcgg cggcatggaa gccgaagctg ttcgaatact
     3901 tcacgatctt gctgacgccg gcgaggcccg cgctggagat caggatgaag gaggcgatca
     3961 gcacccacat ctgccaggta cccagtgcct ccttcagcgt gaactggtgc tcggtcggac
     4021 ggcgcttggc ctcatccggc acccagcccg cggggcgcca gccggggggc ggatccttca
     4081 tgaacagcgc ggccac
//
LOCUS       DASSZH010000636         2027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_731426, whole genome shotgun sequence.
ACCESSION   DASSZH010000636 DASSZH010000000
VERSION     DASSZH010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731426"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="VFN42_13740"
     CDS             <1..1157
                     /locus_tag="VFN42_13740"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET8997725.1"
                     /translation="AFRDGSGRVGVFDHRCPHRCASLFYGRNEEGGIRCIYHGCKFDI
                     DGNCLDMPNVVPRQRSEAKVHAKAYKVTERAGLIWVYMGARAEAPPMPAIEATLLDDA
                     EVDLMFVQRECNWLQALEGDIDTSHFTFLHVGSLQPEQIDDGNMMKYQVANRAPEYQV
                     ADTDWGTMYTAYRPAEEDRTYWRFAHFGFPFWTWVPQGDFLDRVSARAWVPMDDEHTM
                     FLSISWKKMSRMPPLKDGKPIPGSRAKLDYLPNTTDWYGRWRPVASAGNDYFIDREAQ
                     RDGTIFSGITHIHMQDQAVTESMGPITDHAFENLAPSDLMIARTRRRLVVAARALREK
                     GTVPPGVDNPEIFRQVRSGECVLAATDWRAAYADRMQAATRPAGELLAAN"
     gene            1191..>2027
                     /locus_tag="VFN42_13745"
     CDS             1191..>2027
                     /locus_tag="VFN42_13745"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997726.1"
                     /translation="MAKIVLGLATSHGPLLTTPPEQWDERAKADRANTQLWFRGAVHS
                     FDALQAMRAGEGLEDKTSPEERRARSIRCRQALDRLATLFEQARPDAVVIVGNDQQEV
                     FGFGNMPAFAVYRGESVVNRPPSEQQLARMPPGAAIAVFGHRPRHETHYPCAPHLARH
                     MIEQLVAAEFDVAQSNALPQGADGGAQDGLPHALGFVLRQIMQDRPVPHIPVLINTFY
                     PPNQPTARRCHRFGSALADAIAAWSEDLRVAVIASGGLSHFVVDEELDARFIAALGAD
                     DAG"
BASE COUNT          356 a          646 c          695 g          330 t
ORIGIN      
        1 tcgccttccg cgacggcagc ggccgtgtcg gtgtgttcga tcaccgctgc ccgcatcgct
       61 gcgcgtcgct gttctacggc cgcaacgagg agggcggcat ccgctgcatc tatcacggct
      121 gtaagttcga catcgacggc aattgcctgg acatgccaaa cgtcgttcca cgccagcgct
      181 ccgaggcgaa ggtgcacgcc aaggcctata aggtaaccga gcgtgccggg ctgatctggg
      241 tgtatatggg cgcccgcgcc gaagcgccgc cgatgccggc gatcgaggcg acgctgctgg
      301 acgacgccga agtcgacctg atgttcgtgc aacgcgaatg caactggttg caggcgctgg
      361 aaggcgacat cgacacgtcg catttcacct tcctgcatgt cggcagtctg cagcccgagc
      421 agatcgacga cggcaacatg atgaaatacc aggtggccaa ccgcgcgccc gagtaccagg
      481 tggcggacac cgactggggc acgatgtaca ccgcctaccg tccggcggag gaagaccgca
      541 cctattggcg ctttgcgcat ttcggctttc cgttctggac ctgggtgccg cagggcgatt
      601 tcctggatcg cgtttcggca cgcgcctggg tgccgatgga cgacgagcac accatgttcc
      661 tgtcgatttc ctggaagaag atgtcgcgca tgccgccgct gaaggacggc aagccgatcc
      721 cgggctcacg cgcaaaactg gactacctgc cgaacacgac cgactggtac gggcgctggc
      781 gtccggtggc gagtgccggc aacgactact tcatcgatcg cgaggcgcag cgtgacggca
      841 cgatcttctc cggcatcacg catatccaca tgcaggacca ggcggtgacc gagagcatgg
      901 gcccgatcac cgaccacgcc ttcgaaaacc tggcgccgag cgacctgatg attgcacgga
      961 cgcggcggcg gctggtggtg gcggcgcggg cgctgcggga gaagggaacg gtgccgccgg
     1021 gcgtggataa ccctgagatc ttccgccagg tccgcagcgg cgaatgtgtt ctggcagcga
     1081 cggattggcg tgcggcctat gcggaccgca tgcaggcggc gaccagaccg gccggggagc
     1141 tgctggcagc caactgactc ccggctgggc gcggaacgga aggaacagcc atggcgaaga
     1201 ttgtgctggg cttggcgaca tcgcacgggc cgctgctgac caccccgccg gagcaatggg
     1261 acgaacgcgc caaggcggat cgtgccaaca cgcaactgtg gttccgcggc gcggtccatt
     1321 ccttcgatgc cctgcaggcc atgcgcgccg gagaggggtt ggaagacaag acctcaccgg
     1381 aggaacgccg cgcgcggtcc atacgctgcc gccaggcgct ggatcgcctg gcaacgctgt
     1441 tcgaacaggc gcggcccgat gccgtggtga tcgtcggtaa cgaccagcag gaggtattcg
     1501 gcttcggcaa tatgccggcc tttgcggtgt accggggtga atcggtggtg aaccgccctc
     1561 cctcggagca gcagcttgcg cggatgccgc cgggtgcggc gatcgcggta ttcggccatc
     1621 ggccgcgcca cgaaacccac tatccctgcg ccccgcacct cgctcgccac atgatcgaac
     1681 agctcgtcgc ggcggaattc gatgtggcgc aatccaacgc gctgccgcag ggcgctgatg
     1741 gaggggcgca ggacggactg ccgcacgcgc tcgggtttgt gctgcggcag atcatgcagg
     1801 accggccggt gccgcatatc cccgtgctga tcaacacctt ctacccgccc aaccagccga
     1861 cggcacgccg ctgccaccgc ttcggatcgg cgctggcgga tgccattgca gcgtggtcgg
     1921 aggatttgcg cgtggcggtg attgcttccg gcgggctgtc gcacttcgtg gtggatgagg
     1981 aactggacgc acggtttatt gcggccctgg gagccgacga cgcaggt
//
LOCUS       DASSZH010000637         6296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_987095, whole genome shotgun sequence.
ACCESSION   DASSZH010000637 DASSZH010000000
VERSION     DASSZH010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6296
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987095"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            550..1830
                     /locus_tag="VFN42_13750"
     CDS             550..1830
                     /locus_tag="VFN42_13750"
                     /inference="COORDINATES: protein motif:HMM:NF016670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HET8997727.1"
                     /translation="MAPRPALGQQIHLALIQRGAAAAAEIARDVGQPDLFERVNAIAG
                     ETPSPSFLHSALCAMSLPVRRPADEHSPIIRQDGQYTLVISPKPVVETVNGQQCLRSL
                     GVPYGSLPRLVLIHIMTEAVRSRSRHIVLGRNFTDWMRRMGFRTISYGPRGSATLVRE
                     QLDRLLACEWMIRWQNTAEQGEREFAVKEIKLTNEYTGVDRSGGAFVREIVMTEGFFE
                     HLRQHAVPLNEHAIRQLRDSATALDLYTWLAYRLPRINAKRPAAISWQQLAVHFGNDG
                     RNIRKFRQTIRDAWERHVSAVYPEARADFDTTVVRLYASPAPLQQKLVAVPALPIAAD
                     DKRTPPAPPEEAVLDVAGTFLAALTHALGPADARAWLKDVTLEACEDGWVLLTGTPFV
                     AHWIASRFDRALAQAAAAAQLGRVPTVRPRTPQR"
     gene            1855..3039
                     /locus_tag="VFN42_13755"
     CDS             1855..3039
                     /locus_tag="VFN42_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8997728.1"
                     /translation="MDVSGTTTTIADLYARSLNVLESLRAQGVRGEQARRQAPRFPIT
                     RVAELVGRTSAAIREAERDGRLPSVERTASGRRIGYTLAEINQMRAVFGTRPWRTEDD
                     PLSVIAVQNFKGGVGKSTLSVHLAQYLAIRGYRVCLIDCDSQGSSTALFGYVPDLDIG
                     EDDTLYPFFRNAEMTSLAYAVRETHWDGLYLVPANLKLYSSEYELAARVARAEPDLLN
                     RLADGIASIGDHFDVVILDPPPALGTISLSVMRAANALIVPIPPTVVDFTSTTTFLAM
                     LYETMLTLEERRLPIDLRWMRLVAARADEQKSMQRELLGLMRNLFGDMLLRTVLKDSA
                     EIDNASARLMTVYELEQPVTSRETYQRCLTYLNGVNAEVETQIRLTWPSQVERLRAEG
                     IL"
     gene            3097..4116
                     /locus_tag="VFN42_13760"
     CDS             3097..4116
                     /locus_tag="VFN42_13760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET8997729.1"
                     /translation="MAIGNRGFAQGLAAGGAPVASGARLPPRTGILGARDNRLAELAA
                     GKTVTRIHELVDPATCRIWSGHNRDYAALNEESCADLIESFKAQGRQEVPAIVRRVTD
                     DPHHAFEVVCGARRHWTATWMRAHSYPEFRFLIEPRELTDEEAFRIADLENRSRRDLS
                     DYERAADYARAIDRYYDGSQQRMAERLEVSKSWLSRYLELAKLPAEVIAAFGSPHVIG
                     ISHGAVLAPLLRMPPQRERMMAEARALAEEQAGLGAHAAALIAPPAVLRRLTAAARSS
                     SRPVRVAAAECVIRDSDGTIVARGQKVGRGGITLNVPALARQDRDAVLRALVAIVDHF
                     KASAR"
     gene            4157..4714
                     /locus_tag="VFN42_13765"
     CDS             4157..4714
                     /locus_tag="VFN42_13765"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET8997730.1"
                     /translation="MTEPARARMTSDAFIAWAMERPRGERYELVAGEVVAMAPERSAH
                     ALTKGRVFRRLAEAIEAARLTCTAYPDGMAVEIDDATTYEPDVLVRCGPPLPDDAVKV
                     TDPMIVVEVLSPSTRARDAGAKLEDYFRLPSVRHYLIVKTENRTVIHHARDAAGNIAT
                     RIIRNGSIVLDPPGITLPDLFAGAP"
     gene            complement(4749..5882)
                     /locus_tag="VFN42_13770"
     CDS             complement(4749..5882)
                     /locus_tag="VFN42_13770"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HET8997731.1"
                     /translation="MRGPAPQQAETVRVWDAGIRMFHWGLAIAVTAAALTGFVLGRTT
                     LAWHLVAGALVIIAVGWRVVWGLLGPTHARFHSFAYRPAAVLAHVDDLTGRHHRRHLG
                     HNPLGAMMVFGLLAVLTAIGLTGAFALGGMLKQGPLRAFLSYGAGRQSLDIHNALAIL
                     LLVMVGGHLAGVALESWRGRENLVAAMLTGNKPRDPPAETVAAVCARPRLAIGITLGI
                     LAVGTGGIAALGTLPGRGVPPATLDPVFAEQCGACHLAFPPSLAPSATWNGILADLKR
                     HFGADATLSAEQVAHLRRWLDANAAEHWDTLPSHLLRRPAPDGSLRITDTPGWRRMHR
                     HIPDAVFAAKEVYRRSACEACHADAASGRFAPQQIAIPKAGGP"
     gene            6065..>6296
                     /locus_tag="VFN42_13775"
     CDS             6065..>6296
                     /locus_tag="VFN42_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997732.1"
                     /translation="MTTSPNPCRGVRFPAEIIEHAVWLYHCFSRALRDAEQRPAWLRR
                     IDASAVLMGTACGRSDAGKVVSSLNSRRVVLGA"
BASE COUNT         1036 a         2112 c         2134 g         1014 t
ORIGIN      
        1 agcggcttca cgcttgccca actcgggatc gaactgaccc ggctcggctt cactccctcc
       61 cgcggcggca ggcaatgggc cccgtcctcg gtcaaggcat tgctggaccg ggcgcgcgcg
      121 gcgggtctgc tgacggggac atgagaggcg acgtgatccg cctgcgacca gggtttccgg
      181 ccgagttgca ggtctgcaac cgcgatcgca cagcgcggcg aaaccgatga gaacggtacg
      241 cccggccatc gagcgatgca cgcggtacgc cggaccgctg agatgggtac atgccgcccc
      301 ggtctgacga tgatcgcggt acgcgaatcg gatggcgaac cgatgcggag ggtacgcaca
      361 cctaatgcat cctcctatac gagtccgaag gcgaccgcgg acagcggaag gatgtacgct
      421 tatcatcggc gcgtttgcgc ttatgtcacg catcacctgg gatttgtgca aatctctccc
      481 cacgccgcgc tcattgtggt agcggtcggg taacgctcct cgattcgagt cgcgcctgga
      541 gatcctcccg tggcccctcg cccagctctt ggccagcaga tccatctggc gctcattcag
      601 cgcggcgccg ccgcggccgc ggagatcgcg cgcgacgtgg gtcagccgga cctgttcgag
      661 cgggtaaacg ccattgctgg cgagacgccc tcccccagct tcctgcattc cgccctctgc
      721 gcgatgtcct tgccggtgcg ccggccggcc gatgagcatt ccccgatcat ccgccaggac
      781 ggacaataca cgctcgtcat cagccccaag cccgtggtgg agacggtcaa cgggcagcaa
      841 tgcctgcgaa gcctcggcgt gccatacggc tccctgccac gcctggtgct catccacatc
      901 atgaccgagg cggtccgttc ccgctcgcga cacatcgtgc tgggccggaa tttcaccgat
      961 tggatgcggc ggatggggtt ccggacgatt agctacgggc cgcgcgggtc ggccacactg
     1021 gtccgggaac aactcgaccg cctgctggcc tgcgaatgga tgatccgctg gcagaacacc
     1081 gccgaacagg gcgagcgcga attcgccgtc aaggagatca agctgaccaa cgaatacacg
     1141 ggcgtggacc gcagtggcgg cgccttcgtc cgcgagatcg tcatgacgga ggggttcttc
     1201 gagcacctgc ggcagcatgc cgtgccgctc aatgaacacg cgatccggca attacgcgat
     1261 tcggcaacgg ccctcgacct ctacacctgg cttgcctatc gcctgccgcg catcaatgcc
     1321 aagcggccgg ccgcgatcag ctggcagcaa ctggccgtgc atttcggcaa cgacggccgc
     1381 aatatccgca agttccggca gaccatccgc gatgcctggg aacgccacgt ctccgccgtt
     1441 tacccggagg cacgggccga tttcgacacc acggtggtgc gtctctacgc ctcgccggcg
     1501 ccgctgcagc agaagctggt ggcggtgccg gccctgccga tcgctgccga tgataagcgt
     1561 acgccgcccg ctccgcccga ggaggcggtc ctggacgtgg ccggaacgtt cctggccgcc
     1621 ctcacccatg cgctcgggcc ggccgatgca cgggcgtggc tgaaggacgt gacgctggaa
     1681 gcatgcgagg acggatgggt gctgctgact ggcacaccgt ttgtcgccca ctggatcgca
     1741 agccgcttcg accgcgcgct ggcgcaggcg gcggcggcgg cgcaactcgg ccgcgtcccg
     1801 accgtccggc cgcgcacgcc gcagcgctga gcgtcacaca gggatcgctg gcacatggac
     1861 gtgtccggaa ccaccacgac gatcgccgat ctctatgcgc ggtcgctgaa tgtactcgag
     1921 tcactgcggg cgcagggtgt ccggggtgag caggcccgtc ggcaggcccc ccgttttccg
     1981 atcacacgcg tggccgaact ggtgggccgc accagtgccg cgatccggga ggcggagagg
     2041 gatggccggc tgccgtcggt ggaacgcacc gcgtccggcc ggcggatcgg ctacacgctc
     2101 gccgagatca accagatgcg ggcggtgttc ggcacgcggc cgtggcgcac cgaagatgat
     2161 ccgctttcgg tcatcgccgt gcagaacttc aagggtggcg tcggcaagtc cacactctcg
     2221 gtccatctcg cgcagtacct tgccatccgc ggctaccgcg tctgcctgat cgattgcgac
     2281 agccagggat cgagcacggc gctgttcggc tatgtgccgg acctcgacat cggggaggac
     2341 gacacgctct atccgttctt ccgcaacgcc gagatgacga gcctagccta cgcggtgcgg
     2401 gagacgcatt gggacgggct ctatctggtt cccgccaacc tcaagctcta ttcgtcggaa
     2461 tacgaacttg cggcgcgcgt ggcgcgggcc gagcccgatc tgctgaacag gctcgcggac
     2521 gggatcgcct cgatcggcga ccatttcgac gtcgtcatcc tggacccgcc gccggcactg
     2581 ggaacgatct cgctctcggt gatgcgggcc gccaacgcgc tgatcgtgcc gatcccgccc
     2641 accgtggtgg atttcacctc caccacgacg ttcctggcga tgctctacga aaccatgctg
     2701 acgctggagg agcggcggct gccgatcgat ctgcgctgga tgcggcttgt cgcggcgcgc
     2761 gcggacgagc agaaatccat gcagcgggaa ctgctcggcc tgatgcgcaa cctgtttggt
     2821 gacatgctgc tgcgcaccgt gctgaaggac agcgccgaga tcgacaacgc ctcggcacgg
     2881 ctgatgaccg tctatgagct ggaacagccg gtcacctcgc gcgaaaccta tcagcgctgc
     2941 ctgacctacc tgaacggcgt gaatgccgag gtggaaacgc agatccggct tacctggcca
     3001 agtcaggtcg agcggctgcg tgcagagggg atcctctgac ctggttgcag ggctgcaact
     3061 cgcacataaa ccattggttg atctggagtt tctggcatgg cgataggcaa tcgcgggttt
     3121 gcccaaggtc tggccgcagg tggggcgccg gtcgcgtccg gggcgcggtt gccgccccgc
     3181 accgggattc tgggcgcccg cgacaaccgg ctcgctgagc ttgccgcggg caagacggtc
     3241 acgcgcatcc acgaactggt tgaccccgcc acctgccgca tctggtccgg ccacaaccgt
     3301 gactatgccg cgctgaatga ggaatcctgc gcggatctga tcgaaagctt caaggcgcag
     3361 ggacggcagg aagtgccggc catcgtgcgc cgggtgacgg atgatcccca ccacgcgttc
     3421 gaggtggtgt gcggcgcgcg ccggcactgg acggcgacct ggatgcgcgc ccactcctat
     3481 ccggagttcc gcttcctcat cgagccgcgc gagctgaccg acgaggaggc gttccgcatc
     3541 gccgacctcg aaaaccgcag ccggcgcgac ctgtcggatt acgagcgcgc ggccgactat
     3601 gcccgtgcca tcgaccgcta ttacgacggc agccagcagc gcatggccga gcggctggag
     3661 gtgagcaaga gctggctgag ccggtatctt gagcttgcga aactgccggc cgaggtcatc
     3721 gcggcattcg gctcccccca tgtcatcggc atcagtcacg gcgcggtgct cgctccgttg
     3781 ctgcgcatgc cgccgcaacg cgagcgcatg atggcggagg cacgggcgct ggcggaggaa
     3841 caagccgggc ttggcgcgca tgccgccgcc ctgattgcgc ccccggcggt gctgcgacgc
     3901 ctgaccgccg ccgccaggtc gtcgtcacgg ccggtccggg tggctgcggc ggagtgtgtg
     3961 atccgtgatt ccgacgggac gattgtcgcg cgggggcaga aggtgggcag gggagggatc
     4021 acgctgaacg tgccggccct cgcgcggcag gatcgggatg cggtgctgcg ggcactcgtg
     4081 gcgatcgtgg accacttcaa ggcatcggcg cgctgaggcg gtcgcacccg aggcggcacc
     4141 gtgatagtct gaaaccatga ccgaaccggc gcgcgcccgc atgacctccg atgcgttcat
     4201 cgcctgggcc atggagcgcc cgagggggga acgctacgaa cttgtcgccg gcgaggtggt
     4261 ggcgatggcg cccgaacgat cggcgcatgc gctgaccaag gggcgtgtgt tccgccggct
     4321 cgccgaggcc atcgaagccg cccgcctgac ctgcacggca tacccggacg gcatggcggt
     4381 ggagatcgac gacgcgacga cctacgaacc cgacgtcctg gtgcgctgcg gcccaccact
     4441 gccggatgac gcggtgaagg tcaccgaccc gatgatcgtg gtcgaggtgc tgtcgccttc
     4501 cacccgtgcg cgcgatgccg gcgcgaagct ggaggactat tttcggcttc cgtcggtgcg
     4561 tcattacctc atcgtcaaga ccgagaaccg caccgtgatc catcacgcgc gggatgcagc
     4621 cggcaacatc gcgacgcgca tcatccgcaa cgggtcgatc gtcctggacc cgccgggcat
     4681 cacattgccc gatctttttg ccggcgcgcc ctgagtagaa ccgccgcgat ggtccggaat
     4741 gcgggcactc acggcccgcc ggccttcggg atggcgatct gctgcggcgc gaagcgcccg
     4801 gatgccgcat cggcatgaca cgcctcgcag gcgctgcgcc gatagacctc cttggcggcg
     4861 aacacggcgt ccgggatatg ccggtgcatc cgtcgccacc cgggcgtgtc ggtgatgcgc
     4921 agcgatccat ccggcgccgg cctgcgcagc aggtgcgatg gcagcgtgtc ccagtgttcg
     4981 gccgcgttgg cgtcgagcca gcggcggaga tgcgcaacct gctcggccga gagtgtggcg
     5041 tccgccccga aatgccgctt caggtcggcg agaattccgt tccaggtagc cgacggcgcc
     5101 aggctcggcg ggaaggccag gtggcaggcg ccgcattgct ccgcgaacac cgggtccagc
     5161 gttgctggcg gcacgccgcg ccctggcagc gtccccaggg ctgcaatccc gcctgtgccg
     5221 acggccagta tcccgagcgt gatcccgatt gcgaggcgcg gccgggcgca tacagccgcc
     5281 acggtttccg ctggcgggtc gcgcggcttg ttgccggtca gcatggcagc aaccaggttt
     5341 tcccgcccgc gccagctttc taacgcgacg cctgccagat ggcccccgac catgaccagc
     5401 agcaggattg ccagtgcgtt gtggatgtcg agagattgcc gccctgcgcc gtaggacagg
     5461 aacgcccgca gcggcccctg cttcagcatc ccgcccaggg caaacgcacc ggtcagcccg
     5521 attgcggtca gcaccgcaag cagcccgaac accatcatgg cgcccagcgg gttgtggccg
     5581 agatggcggc ggtgatgacg gccagtcagg tcgtctacat gcgcgagcac ggccgcaggc
     5641 cggtatgcga agctgtggaa ccgggcatgg gtcgggccga gcagtcccca gacgacgcgc
     5701 cacccgacgg cgatgatgac cagggcacct gcgaccagat gccaggcaag cgtggtgcgc
     5761 ccgagcacga agccggtcag tgccgcggcc gtgaccgcga tggccagacc ccaatggaac
     5821 attcggatgc cagcgtccca tacgcgcacc gtttcagcct gctgcggcgc gggaccgcgc
     5881 acgattcgcc ttcggcgtgt ccacagcgac gcgccaagcc agcccgcatg tgtccagcgt
     5941 tgcatggagg gagcataagg gcgcatgacg gccggacaca ggcggattga tgccaagacc
     6001 acgccggtgg tcttgatgga gttgttagat tgacgaacat ggccgacgcg gcagcgtgcg
     6061 gggcatgacg acctcgccca atccctgccg cggcgtccgc tttccagccg agatcatcga
     6121 gcatgcggtc tggctgtatc attgtttcag ccgtgccctg cgcgatgccg agcagcgccc
     6181 ggcttggctg cgtcggatcg acgcctccgc tgttttgatg gggaccgcgt gcggtcggtc
     6241 cgatgccggc aaggttgttt cgtctctcaa ttcaaggcga gtggtgcttg gagcgc
//
LOCUS       DASSZH010000638         5371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_987099, whole genome shotgun sequence.
ACCESSION   DASSZH010000638 DASSZH010000000
VERSION     DASSZH010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987099"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VFN42_13780"
     CDS             complement(<1..302)
                     /locus_tag="VFN42_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997733.1"
                     /translation="MADTPDPTPVTAQTRFQQAMQQTFALPADPALQLWTKLTAFLRD
                     KPQLRDLPLAFGTTADLNDGYSRVEYRIAPYVRSLAVPVLGAMGVMALGIALDFGA"
     gene            complement(375..2501)
                     /locus_tag="VFN42_13785"
     CDS             complement(375..2501)
                     /locus_tag="VFN42_13785"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HET8997734.1"
                     /translation="MNLDHPLHSFFWPRSIAVLGASPDLHRIRGRLLHQLRENGFPGR
                     ILPINPSYQEIESLRCFPSIGAVGEPIDLALVAIPAAGVAPALEECAAAGVKNTLIIS
                     SGFAEEGGAASAMQAALVEVTARTGIRACGPNCEGYYNALGRVATTFSPTVETQDDDS
                     RVLVSQRRLGVIAQSGGIGFALFNRGKAAGLGFSYVISTGNEADLNMADFLDYMVEDP
                     HTDAVMLFCEAVRNGPDFVNALAKARQRGKPVIAIKIGRSAAGSRASASHTASLSGDY
                     AAYRAVFQRYGVIEAEDADEAVAIAGVVLTCTPPRGRRVGIITPSGGGGAWMADTLSL
                     HGLTVPPLSADTQARLRAVMPSYGAPANPVDVTAQGSATGPAVMTAMEQLAGSDEVDM
                     LVLISSMASTTRATLDAARVRAVAESSGKPMTVWTYTLPSEFGRASAAGCGLFVHSGL
                     RQVGVSLAKLADYAEALARPLPADPRPRDVAPLPPDATGVLPEYHAKRALAAFLPAMR
                     ERLATTAAEAADAASALGFPVALKVQSPDLPHKTEAGGVKLGLVDAAAVRAAYDAMLR
                     DVLEYRPAARIDGVLVQKMAPKGHELVIGMVNDPSFGPIMMLGFGGTTVELFGDVVHS
                     PAPVDAAEATRMLLSLKSAPLLTGFRGTAEVDLAPVAALVAQLSEAALVYRDQIAEME
                     FNPVILHADGSGLTIADALVTLTQTA"
     gene            2706..2870
                     /locus_tag="VFN42_13790"
     CDS             2706..2870
                     /locus_tag="VFN42_13790"
                     /inference="COORDINATES: protein motif:HMM:NF013853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 family protein"
                     /protein_id="HET8997735.1"
                     /translation="MRAGASSALVNRLTMLCLHLLKWRFQPSHRGTNWQLTIKKQPIA
                     WMCSSATIRA"
     gene            complement(3036..3794)
                     /locus_tag="VFN42_13795"
     CDS             complement(3036..3794)
                     /locus_tag="VFN42_13795"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET8997736.1"
                     /translation="MALLHRDGVAIHYEVHGSGPTVLLSHGYGATCRMWDGQIAAFRD
                     RWKLIVWDMRGHGQSDSPADPAAYSEAATVGDMHAILQACAVSRAVIGGLSLGGYMSL
                     AFHLSHPDMVRALMLFDTGPGFRKDDARETWNARARGRGDELDTRGLAALGASDEVRM
                     SQHRSAAGLAGAARGMLTQRDDSVIRSLENIRVPTLVLVGANDTNFLAATDYMAAKIP
                     GAVKAVIPDAGHAANLHQPAAFSRAMEAFLTTIR"
     gene            3896..4678
                     /locus_tag="VFN42_13800"
     CDS             3896..4678
                     /locus_tag="VFN42_13800"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET8997737.1"
                     /translation="MQHSFEHIATSVEGLIGIIEVRRGPNNFVDTDMVGEIADALEAW
                     DRSLDPRAIVLCAEGKHFCAGADFGSRGPDGVARGTKRGRHLYKEAQRLWRTKKPIVA
                     AVHGAAIGAGVGLAVMADFRVTCPEARYSTNFTRLGFHPGFGLTCTLPRLLGQQKAAW
                     MMYSGRRLTGEEALAIGLADYCVPQDQVRAKAIEVATEIAQSSPLGIISTRETLRRGL
                     ADAVAAATEREYEEQDWLRNTEDFKEGVKAMAERRLPDFKGR"
     gene            4712..>5371
                     /locus_tag="VFN42_13805"
     CDS             4712..>5371
                     /locus_tag="VFN42_13805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8997738.1"
                     /translation="MKVGILQFFGWRDRSVPLTSVYETALERFAIMDQAGYDAVWLAE
                     HHFSGFSVCPSVHMMGTMAAARTKRLRIGTAVSLAPFYNPLRLAEEVALLDVLSGGRV
                     NWGAGRGFERSEFAAFGIPGEESAPRFHETVDIVLQAWTSQRITYQGRFFQYDGVEVL
                     PKPLQAPHPPVWMAASSIPAIEWAAGQGHSILMDPHSSRADLIRKRRHYAAKLAEAGY
                     SD"
BASE COUNT          928 a         1823 c         1739 g          881 t
ORIGIN      
        1 aatgctccga aatcaagtgc gattcccaat gccatcaccc ccatggcgcc cagcaccggc
       61 acggcaaggc tcctgacata gggcgcgatg cgatattcga cacggctgta gccgtcattg
      121 aggtctgcgg ttgtgccgaa cgccaatggc aggtcgcgca attgcggctt gtcccgcagg
      181 aaagccgtga gcttcgtcca caattgcaac gcaggatccg ccggcagcgc aaacgtctgc
      241 tgcatcgcct gttggaaccg ggtctgtgcc gtcaccggcg tggggtctgg ggtgtcagcc
      301 acggatctct cctcttgctg gtggtcaacc tttggtcata ctgcaaaggt ctcgttccgt
      361 tgccggctct gccctcacgc cgtctgcgtc aacgtcacca gcgcatcggc gatcgtcagt
      421 cccgatccgt cggcgtgcag gatcaccgga ttgaactcca tttcggcgat ctgatcgcga
      481 tacaccagcg ccgcctcgga aagttgcgcg accaaggccg cgacgggcgc cagatctacc
      541 tccgcggtgc cacgaaatcc ggtgagcagc ggcgcggatt tcagcgacag cagcatccgg
      601 gtcgcttccg cggcatccac cggggccggg ctatgcacca cgtcgccgaa taactccacc
      661 gtggtgccgc caaagccgag catcatgatg gggccgaagc tcgggtcgtt caccatgccg
      721 atgaccagct catggccctt cggcgccatc ttctgcacca gcacgccgtc gatgcgggca
      781 gccggcctgt attccagcac atcgcgcagc atcgcgtcgt acgcggcacg cacagccgcc
      841 gcgtccacca aacccagctt cacaccaccg gcttccgtct tatgcggcag gtcgggtgat
      901 tgcaccttca acgccactgg aaagcccagc gcggacgcgg catcggcggc ctccgccgcg
      961 gtggttgcca gccgctcccg catcgccggc aggaacgccg ccagcgcacg cttggcatgg
     1021 tattcgggca gcacgcccgt cgcgtcgggc ggcagtggcg cgacgtcacg cggtcgcgga
     1081 tcggcgggca gcggacgtgc cagcgcctcg gcatagtccg cgagcttcgc cagtgacacc
     1141 ccgacctggc gcaggccgct gtgcacaaac agaccgcacc ccgccgccga agcccggccg
     1201 aattcgctgg gcaacgtata ggtccacacc gtcatcggct tgccggaact ctccgcgaca
     1261 gccctcaccc gcgcggcatc caacgtcgcg cgggtggtgc tggccatcga gctgatcagc
     1321 accagcatgt ccacttcatc cgatccggcg agttgctcca tcgccgtcat caccgccgga
     1381 ccggtcgcgg agccctgcgc cgtgacatcc accggattgg cgggtgcgcc ataggacggc
     1441 atgacggcgc gcagccgcgc ctgcgtgtcg gccgacaacg gcggcaccgt caggccgtgc
     1501 agcgacaacg tgtcggccat ccacgcaccg ccgccgccgg acggcgtgat gatgccgacg
     1561 cggcggcctc ttggtggcgt gcatgtcagt accacgcccg cgatcgccac cgcttcgtcg
     1621 gcatcctcgg cctcgatgac gccatagcgc tggaacaccg cacgatacgc ggcataatcg
     1681 ccggacagag acgccgtatg cgaagcactg gccctgctgc cggcggcgga gcgaccgatc
     1741 ttgatggcga tcaccggttt gccgcgctgc cgcgccttgg ccagcgcatt gacgaagtcg
     1801 gggccgttgc gcaccgcttc gcagaacagc atcaccgcat cggtgtgcgg gtcttccacc
     1861 atgtagtcca ggaagtccgc catgttcagg tcggcttcgt tgccggtgga gatcacgtag
     1921 ctgaagccca gccccgctgc cttgcctcgg ttgaagagcg cgaagccgat gccgccggac
     1981 tgcgcgatca cgcccagccg gcgctgcgac accaggacgc ggctgtcgtc gtcctgagtt
     2041 tccacggtgg ggctgaacgt ggtggcgacg cggcccagcg cgttgtagta gccctcacag
     2101 ttcggaccgc aggcgcggat gccggtgcgg gcggttacct ctaccagcgc ggcttgcatg
     2161 gcacttgccg cgccgccctc ctcggcaaaa cctgagctga tgatcagcgt gttcttcacg
     2221 ccggctgcag cgcactcctc cagcgcgggg gcaacgccgg cagcgggaat cgccaccagc
     2281 gccaggtcga tcggctcgcc aaccgcgccg atgctgggaa agcagcgcag gctttcgatc
     2341 tcctggtaac tgggattgat tggcaggatg cggccaggaa agccgttctc gcgcaattgg
     2401 tgcagcagcc gtccgcggat gcggtgcagg tcgggagagg cgccgagcac ggcaatggat
     2461 cgcggccaga agaaactgtg gagcgggtgg tcaaggttca tcggctggct cctcccgaag
     2521 ccgaaatgct gcacgcgttc attcagccta tcgcgagacg tcccgctatg accatatccg
     2581 gacagcgtcc aaccgccggt gcattccgct atggagcgga cctctggaga accggagacg
     2641 acgctatgtc aaacagcctc tatgctcgga atttctacgc ataggccacc gaacacacgg
     2701 cgctgttgcg ggccggagcg agtagcgcgc tggtcaatcg cctcaccatg ctgtgtctgc
     2761 acctgttgaa gtggcgcttc caaccgagcc atcgtggcac aaactggcag ctgacgatca
     2821 agaaacagcc tatcgcctgg atgtgcagct ccgcgacaat ccgagcctga agtcccatct
     2881 cgatgacgcg atgcgggatg cctatcggct cgcctgcgtc gaagcgtgac gaaaccgggc
     2941 tggccgaaga cgccttccca gccagttcgc ttttccgttt cgatcaggtt ctcgccccgg
     3001 aagtccccag cgcaggaggg agagacgggc tcacgtcatc tgatcgtcgt gagaaacgcc
     3061 tccatcgcgc ggctgaacgc cgccggctgg tgcagattgg cggcgtggcc ggcatccggg
     3121 atcaccgcct tcaccgcgcc cggaattttc gccgccatgt agtcggtcgc ggcgaggaaa
     3181 ttggtgtcat tcgcaccgac cagcaccagc gtcggcacgc ggatgttttc cagcgaccgg
     3241 atgactgaat cgtcccgctg cgtcagcatg cctcgcgctg ccccggccag accggcggca
     3301 gaacggtgct ggctcatacg cacctcgtca ctggcgccca gggcggcaag gccgcgtgtg
     3361 tccaattcgt ccccgcggcc gcgggcgcgt gcattccagg tttcccgtgc gtcatccttg
     3421 cgaaaacccg ggccggtgtc gaacagcatc aatgcccgga ccatgtccgg gtggctcaga
     3481 tggaaggcca acgacatgta gccgcccagc gacaagccgc ctatcaccgc gcgagacacc
     3541 gcgcaggcct gcagaatggc atgcatgtcg ccgaccgtcg cggcctcgga atacgcggcc
     3601 gggtcggcgg gtgaatcgct ctggccgtga ccgcgcatat cccacacgat cagcttccaa
     3661 cggtcgcgga atgcggcgat ctggccgtcc cacatgcggc aggtggcgcc gtagccgtgg
     3721 ctcagcagca ccgtgggacc ggagccgtgt acctcgtaat gaatcgccac gccgtcacgg
     3781 tgcagcagag ccatggttcc ctccgtcccg ggccacgcat tgtcggcgcg accttccgct
     3841 cataagctgg cacgcagcgc aacacagcgg aagagctcgg caggaaaccc agatcatgca
     3901 gcatagcttc gaacacatcg ccaccagcgt ggaaggcctg atcggtatca tcgaggtccg
     3961 ccgcgggccc aacaacttcg tcgataccga catggtgggc gagatcgccg acgcgctgga
     4021 ggcgtgggac cgctcgctcg acccgcgtgc catcgtgctg tgcgccgagg gcaagcattt
     4081 ctgcgccggt gccgatttcg gcagccgcgg cccggacggc gtggcacgcg gcaccaagcg
     4141 cgggcggcac ctgtacaagg aggcgcagcg gctgtggcgc accaagaagc cgatcgtcgc
     4201 cgcggtgcac ggcgcggcga tcggtgccgg cgtgggcctg gcggtgatgg ccgatttccg
     4261 cgtcacctgc ccggaggcgc gctattccac caacttcacg cgcctgggct ttcatcccgg
     4321 gttcggcctg acctgcaccc tgccgcggct gcttggccag cagaaagcag cgtggatgat
     4381 gtacagcggg cggcgactga ccggcgagga agcgctggcg atcggcctgg cggattactg
     4441 cgtcccgcag gaccaggtgc gcgccaaggc gatcgaagtg gcgaccgaga tcgcgcaatc
     4501 ctccccgttg gggatcatct ccacgcggga aacgctgcgc cgcggtctgg ccgacgccgt
     4561 cgccgcagcc accgagcgcg aatacgagga gcaggactgg ctgcgtaaca ccgaggactt
     4621 caaggaaggc gtcaaggcga tggccgagcg gcggttgccg gatttcaaag ggcgctgacc
     4681 gcgccgcgca aagagctgcc agggggaaat catgaaggtc ggcattctgc agttcttcgg
     4741 gtggcgcgac cgttcggtgc cattgacttc ggtgtatgag acggcgctgg agcgcttcgc
     4801 tatcatggac caggccggct acgacgcggt ctggctggcg gagcaccatt tctccggctt
     4861 ctccgtctgc ccgtcggtgc acatgatggg caccatggcc gccgcgcgca ccaagcggtt
     4921 gcgcatcggc accgcggtgt cgctggcacc gttctacaat ccactgcgcc tggccgagga
     4981 agtcgcgctg ctggatgtgc tgtccggcgg ccgggtgaac tggggcgccg gccgtggctt
     5041 cgaacgcagc gagttcgctg cgttcggcat cccgggggaa gaaagcgccc cacgcttcca
     5101 cgaaaccgtc gatatcgtgc tgcaggcctg gaccagccag cgcatcacct accagggccg
     5161 tttcttccag tatgacggcg tggaggtgct gcccaagccg ctgcaggcgc cacatccgcc
     5221 ggtatggatg gcggcgtctt ccataccggc catcgaatgg gcggcgggcc agggccattc
     5281 catcctgatg gacccgcact cctcgcgcgc cgacctgatc cgcaagcgcc ggcattatgc
     5341 ggcaaagctc gcggaagccg gctacagcga t
//
LOCUS       DASSZH010000639         8835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_135096, whole genome shotgun sequence.
ACCESSION   DASSZH010000639 DASSZH010000000
VERSION     DASSZH010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8835
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135096"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VFN42_13810"
     CDS             <1..305
                     /locus_tag="VFN42_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997739.1"
                     /translation="LAQLSDALRTSDLRTLLSRAEALSRRQPMLVAGAAFAAGFAAMR
                     ATRVGLHAASGSVSEVAGNAMETARREAGNVMAGARNQPDATTPTEAGTGRSSREH"
     gene            295..684
                     /locus_tag="VFN42_13815"
     CDS             295..684
                     /locus_tag="VFN42_13815"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET8997740.1"
                     /translation="MNIEHRMSLREVVGGLADDAGSLVRGEVRLARAELEQKTDRLIA
                     GLVSLIGAMFLAYAGLIVLLLGVARALALAIPLWAALLAIGFVVLAIGVALALGARKA
                     LAPSGMVPDRTAANLQADAAVVKEHAA"
     gene            681..1667
                     /locus_tag="VFN42_13820"
     CDS             681..1667
                     /locus_tag="VFN42_13820"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HET8997741.1"
                     /translation="MSDHVNDPAQIERELTETRARLGSHLDELSRRLSPGQLLDEGLA
                     YLRDGQGATFARNLGAQVRDNPLPVALTGIGIAWLALAGSVGGNRIPGSASRALVPYR
                     DAGAGVHDARDDLADRAHRAGAAVTRATGETADAFSARVSDAQAKVLGLTRSAEETAT
                     AFADRVRQSLDRARQTAASWRDSAAEGMQRTGEAMNRARDNSARIGDRASQAGAAYAS
                     NAGSRLAAALSDNPLLLSAVGLTAGAVFGALLPETRLEERQLGPAATHVADAAQGLAE
                     DVLQRGANVARAAAQAGIDAARREGAGLSGKPGDEPRRKGNGSGGDQAGAAM"
     gene            1891..3171
                     /locus_tag="VFN42_13825"
     CDS             1891..3171
                     /locus_tag="VFN42_13825"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET8997742.1"
                     /translation="MSRHRLSQNRGAGNRHAHPQNVHAGHGQPAHGNLSQHQLPQARQ
                     SHAAGSLLPVQNRRFVPQHFPFQPLPPPNGASPFHFDLTQLLHPDEVQKITRDGRLVF
                     HTVGDTGDERGKEMDFVAHMMTSDYDASADGAVPAFFYHLGDVVYFAGDIDKYGACFY
                     ETYAEYPALIVAIPGNHDCQPDDPQDGPVDPNKKPLDGFVQNFMSKDPTQLGSLKTTS
                     SRTQMNLPNVYWTFTTPFATIIGLFSNVGETEAELHQNQIDWLKSELKAADADKALIV
                     AIHHPPFSGDTEHSGSTVSEHVLFNSFAETGVYPHMILSGHVHNYQRFTVRQQVNGKV
                     AAIPCIVAGNGGYSKLGKLHKLHGNWPARGLQLSDTLTLETYDQDNFGFVRFEVTKDE
                     IIGRYFSAPFAEERTPQTQQMDSFSINLASRTVS"
     gene            3248..4276
                     /locus_tag="VFN42_13830"
     CDS             3248..4276
                     /locus_tag="VFN42_13830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8997743.1"
                     /translation="MQFGLFGAASAQRGGPDVDSAAGFKDYIEYLIEAEALGYHSSFI
                     VEHHFTGFGQVSATLNLLTWIGARTRNLRLGTAVIVLPWHNPVLLAEQAATIDLLSGG
                     RLDFGIGKGYRHNEFASFRIPMEEAEPRFEEAIDIITQSWTSNERFNFHGKYWQFDDI
                     IVEPPTYQKPHPPFWQGAGHPESISRVAKRGHNLLVDQFCPLNVAAERVALYKAAMQQ
                     HGFAYDPSMAGVARAFHVARDAADKDAAIERRLVAQRRMTEISQRPDGKNTASIMAYS
                     DTKAASEASALYGSPDEIARKLETLRDQGVEYVLLNGGGTSRDNLRRFAREVMPAFRT
                     EPALRQAG"
     gene            4283..5131
                     /locus_tag="VFN42_13835"
     CDS             4283..5131
                     /locus_tag="VFN42_13835"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8997744.1"
                     /translation="MTDRVAIVTGGLRGLGRAMALGLVRSGVRVLAVGHIDSDIPEMQ
                     ALLRADEADRLDCLVADIRKPAECDRVLAACLERFSRVDILVNSAGLTFTYIWPDRFR
                     RDTPQKFWEAKDAVIQAVMDTNYVAADQMTRRVAPLLVQQGWGRIVSVTTKLDTMNRI
                     GTTPYGASKAALEMATEVWAKELAGTGVTANIVNPGAGANTPGMAQEMRDWSREGKAP
                     RLVEAEEMVPPLLYVASAEADAVNGYRFDANTWDTALPPTEAARRNARPAGFVLHPQD
                     AANWPG"
     gene            5165..6358
                     /locus_tag="VFN42_13840"
     CDS             5165..6358
                     /locus_tag="VFN42_13840"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET8997745.1"
                     /translation="MTLPLAGIRVLDLSSIIAAPVTATMLGDFGAEVVKVEDPIHGDF
                     MRRGAREPGGRSLQWVQDARNKESVTLNLRHAEARAIVHRLLPHFDVVVTNFRPPTLR
                     RWGFDPDTVRARHPRLIALYVTGYGLTGPYSDRGAFDRVASAFAGLTHVTGESDRPPV
                     RSGYATIDYMGAYAGAFGVMTALYHRDCRGGSGQVIDLALYEPGFRASEDALLAYAAT
                     GRVRERSGNINPKVVPAGDFDTADGKRVTIHAGTQPLLRRLAELMNEPGLCDDPRFAT
                     HARRVENQDALYATIADWVSRQNLTDLMASLVAADIPASPLMSVADIAADPHFRERGT
                     IMPVQDEDHGALLMAAPIPRLSETPGSVRSLGPRLGSANETIFGGLLAMSADEIARLR
                     QAGTI"
     gene            complement(6365..7408)
                     /gene="queA"
                     /locus_tag="VFN42_13845"
     CDS             complement(6365..7408)
                     /gene="queA"
                     /locus_tag="VFN42_13845"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008852908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HET8997746.1"
                     /translation="MPDGMAAFDFALPAERIAQHPARPREAARLLHVTAGGLGDHVVR
                     DLPDLLRPGDLLVANDTKVIPAQLRARRGAAQVGITLDRPLPDGSWHALARNARRLHP
                     GDTLRFEGADDLSATVQQRDPDGGVALRFNLEGAAFGQALQRAGALALPPYIARPHGP
                     LPEDASDYQTVFAAREGAVAAPTAGLHFTPALLAALEARGIGRTTLTLHVGAGTFLPV
                     RGDLAQHRIHAERGEIGPDTAAAINAAREAGGRIVAVGTTSVRLLETAAEEGGRIRPF
                     AGDTELFIKPGYRFRAIDLLLTNFHLPRSTLFMLVCVFAGTARMQAAYAHAVEAGYRF
                     YSYGDACLLERGE"
     gene            complement(7420..7968)
                     /locus_tag="VFN42_13850"
     CDS             complement(7420..7968)
                     /locus_tag="VFN42_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VUT family protein"
                     /protein_id="HET8997747.1"
                     /translation="MQNDRRRLIGFAAFACFLGSIPLANWMIQHVGTVCTLHGPCLVP
                     VAPGILAPSGVLTVGAALVLRDVLQRCLGLYWGLAAIALGTTLSTLVAPGALVIASGT
                     AFALSELADFAVYTPLQRRRLVTAVVASSLVGLVVDSIVFLILAFGSLEFLPGQVIGK
                     LWAVLIAVPLVRLLRRVAPTPA"
     gene            complement(7975..8367)
                     /locus_tag="VFN42_13855"
     CDS             complement(7975..8367)
                     /locus_tag="VFN42_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006558909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8997748.1"
                     /translation="MRFTIDRIDHLVLNVKDVEIAAAWYQRVLGMEREEFGPDRRTAL
                     KFGGQKINLRPADADPGSWPTGVNDLPGSGDVCFITAVPPDEVVAHLHDCGVTILIGP
                     SNRAGALGAIKSVYCRDPDGNLVEIASY"
     gene            complement(8576..>8835)
                     /locus_tag="VFN42_13860"
     CDS             complement(8576..>8835)
                     /locus_tag="VFN42_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997749.1"
                     /translation="RKSNAVRPRDDDEPVPDWAAEAEFYAKVYPDRARLIRRLGRLPI
                     NCDFGPPEPKLLRAIIIGTTPSLLELDVPDPAAEERLRRYA"
BASE COUNT         1497 a         3025 c         2999 g         1314 t
ORIGIN      
        1 aactggcaca gctctccgat gcgttgcgca ccagcgatct gcgcacgctt ctcagccgtg
       61 cggaagcatt gtcgcgccgc cagccgatgc tggtggccgg tgcggcgttt gccgccggct
      121 tcgccgccat gcgcgccacc cgtgtcggcc tgcatgcagc gtccggcagc gtatcggaag
      181 ttgccggcaa tgccatggaa acggcgcgcc gcgaagccgg caacgtgatg gccggcgctc
      241 gcaaccagcc tgacgccacc acgccaaccg aagcgggaac cgggaggagc agccgtgaac
      301 attgaacacc gcatgagtct gcgcgaagtg gttggcggcc tggccgatga cgccggcagc
      361 ctggtgcgcg gcgaggtcag gctcgcccgc gccgaactgg aacagaagac cgaccggctg
      421 attgccggac tggtctcgct gatcggcgcc atgttcctcg cttatgccgg actgatcgtg
      481 ctgctgcttg gcgtggcccg ggccctggcg ctggccattc cgctctgggc cgccttgttg
      541 gcgatcggct tcgtcgttct ggccattggc gtcgcgctgg cattgggcgc acgcaaggcg
      601 ctggcaccgt ccggaatggt gccggaccgc accgcggcga accttcaggc cgacgccgcg
      661 gtggtgaagg agcacgcagc atgagcgacc atgtcaacga cccggcgcag atcgaacgtg
      721 agttgacgga aacccgcgca aggctgggca gccacctgga cgagttgtcg cgccgcctgt
      781 cgcccggcca gttgctcgac gagggcctgg cgtatttgcg cgatggccag ggcgccacat
      841 tcgcccgcaa cctcggcgcg caggtgcggg ataacccgct gccggtggcc ctgacgggca
      901 tcggcatcgc ctggctcgca ctggccggca gtgtcggcgg caatcgcatt cccggctccg
      961 cctcacgcgc tctggtgccg tatcgcgatg ccggcgcggg ggtgcatgac gcgcgcgatg
     1021 atctggccga tcgcgcgcac cgcgcgggtg cggcagtgac ccgcgcgacc ggcgaaacgg
     1081 cagacgcctt cagcgcgcgt gtctccgatg cgcaggcaaa ggtgctgggc ctgacgcgca
     1141 gtgccgagga aaccgccacc gcctttgccg accgcgtcag gcagagcctg gatcgggcgc
     1201 gccaaaccgc cgcgtcctgg cgtgattcgg cggccgaagg catgcagcgg accggcgagg
     1261 cgatgaaccg cgcgcgcgac aactcggcgc ggatcggcga ccgggcctcc caggcgggcg
     1321 ccgcctatgc gtcgaatgcg ggcagccggc tggcggccgc gctgagcgac aatccgctgc
     1381 tgttgtcggc cgtgggcctg accgcgggcg cagtgttcgg cgccttgttg cccgagacgc
     1441 ggctcgagga acgccaactg ggtccggccg ccacgcacgt ggcggatgcc gcgcagggat
     1501 tggcggagga tgtgctgcag cgcggcgcca acgtggcacg cgcggccgcc caagccggca
     1561 tcgacgcagc gcgcagggaa ggcgcgggtc tgtcgggcaa acccggcgac gaaccccggc
     1621 gcaagggcaa tggcagcggg ggcgatcaag cgggtgctgc catgtagcct gctgctacgg
     1681 cgcacggtgt cgcatcgcgg gccttccgcc aggagaaggc ccgccacatc gatcttggca
     1741 cgcgtgcagc gggcggcgcc ttcctgaatc gtcccccttc agtgacgttt cgttggcagt
     1801 gtgacttagc tcatacgttc ctccggcggt gcggcctcat gttccggctg agtattgccc
     1861 tcgggcagac tgcccggcca ggaggacgtc atgagcagac atcgtctcag ccagaatcgc
     1921 ggcgccggaa accggcatgc acacccgcag aacgtccatg cagggcacgg acaaccggcg
     1981 catggcaacc tgtcgcagca tcagctgccg caggctcgcc agtcacacgc cgccggatcg
     2041 ctgctgccgg tgcagaacag gcgcttcgtc ccgcaacatt tccccttcca gcccttgccg
     2101 ccgccgaacg gcgcatcgcc gttccatttc gatctgacgc aactgctgca tccggacgaa
     2161 gtccagaaga tcacccggga cggcaggctg gtgtttcaca cggtcggcga cacgggtgac
     2221 gagcgcggca aggaaatgga cttcgtcgcc cacatgatga ccagcgatta cgacgccagc
     2281 gccgacggag ccgtgccggc cttcttctac cacctgggcg acgtggtcta ctttgccggc
     2341 gacatcgaca agtatggcgc atgtttttac gaaacctatg ccgagtaccc cgcgctgatc
     2401 gtggccatcc ccggcaacca tgattgccag ccggacgatc cgcaggacgg gccggtcgat
     2461 ccgaataaga agccgctcga cggcttcgtg caaaacttca tgtcgaagga cccgacccag
     2521 ctcggctcgc tgaagacgac ctcgtcccgc acgcagatga acctgccgaa cgtctattgg
     2581 accttcacca ccccctttgc cacgatcatt ggcctgtttt cgaacgtcgg cgaaaccgag
     2641 gccgaactgc accagaacca gatcgactgg ctcaagtcgg aactgaaggc ggcggatgcc
     2701 gacaaggcac tgatcgtcgc catccatcac ccgccattct cgggcgacac cgaacactcg
     2761 ggcagcacgg tctccgaaca cgtgctgttc aacagctttg ccgagaccgg cgtatacccc
     2821 cacatgatcc tgtccggcca cgtgcacaac tatcagcgct tcacggttag gcaacaggtg
     2881 aacggcaaag ttgctgcgat cccctgcatc gttgccggca atggcggata ttcgaagctc
     2941 ggcaagctgc acaagctaca cggcaactgg ccggcgcgcg ggttgcagct gtccgatacc
     3001 ctgacgctgg aaacctacga tcaggacaac ttcggtttcg ttcgcttcga agttaccaag
     3061 gatgagatca ttggccgcta cttctcggcg ccgttcgccg aggagcggac accacagacc
     3121 cagcagatgg acagcttcag catcaatctg gccagccgca ccgtaagcta ggctcttgcc
     3181 gtacccggcg gctcctgggc aaagtccggt ccaggtccag gctcctgggc ccagggagag
     3241 acaacgcatg caattcggac tgttcggcgc cgcctcggcg cagcgcggcg gcccagatgt
     3301 ggacagcgcc gccgggttca aggactacat cgaatacctg atcgaggccg aagcgctcgg
     3361 ctaccacagc agtttcatcg tcgagcacca tttcaccggc ttcggccagg tctccgccac
     3421 gctcaacctg ctcacctgga tcggcgcccg cacgcgcaac ctgcggctcg gaacggcggt
     3481 gatcgtgctg ccctggcaca atccagtgct gctggccgaa caggcggcga caatcgatct
     3541 gctcagcggc ggccggctgg atttcggcat cggcaaaggc taccgccaca acgaatttgc
     3601 cagtttccgc atcccgatgg aagaagccga accgcgcttc gaggaagcca tcgacatcat
     3661 cacccagtcc tggacctcga acgaacggtt caacttccac ggcaagtact ggcagttcga
     3721 tgacatcatc gtggagccgc caacgtatca gaagccgcac ccgccgttct ggcagggcgc
     3781 cggccatccg gaatcgatca gccgcgtggc gaagcgcggg cacaacctgc tggtggacca
     3841 gttctgcccg ctgaacgtcg ccgcggaacg cgtcgcgctg tacaaggcgg cgatgcagca
     3901 gcacggcttc gcctacgatc catcgatggc gggcgtggca cgcgcgttcc atgtcgcgcg
     3961 ggacgcggcg gacaaggacg cggcgatcga acgccgcctc gtcgcgcaac gccgcatgac
     4021 ggagatcagc cagcgcccgg acggcaagaa caccgccagc atcatggcct attccgacac
     4081 caaggccgcc agcgaggcca gtgcgctgta cggcagcccg gacgagatcg cgcgcaagct
     4141 ggagacgctg cgcgaccagg gcgtggaata cgtgctgctg aacggcggcg gtacatcccg
     4201 cgacaacctg cggcgcttcg cccgcgaggt gatgccggcg ttccgcaccg agcccgcact
     4261 gcggcaggcg gggtaagcac gcatgaccga tcgtgtcgcc atcgtcaccg gcgggctgcg
     4321 cgggctcggc cgagcgatgg cactcggcct ggtgcgcagc ggggtgcgcg tgctggctgt
     4381 ggggcatatc gacagcgaca ttcccgagat gcaggcgctg ttgcgcgcgg acgaggcgga
     4441 tcggctggac tgcctcgtgg cggacatccg caagccggcg gaatgcgacc gggtgctggc
     4501 cgcatgcctc gagcggttca gccgggtgga tatcctggtc aacagcgccg gcctgacctt
     4561 cacctatatc tggcccgacc gtttccgccg cgacacgccg cagaagttct gggaggcaaa
     4621 ggacgcggtg atccaggcag tgatggacac caactacgtc gccgccgacc agatgacgcg
     4681 ccgtgtggcg ccgctgctgg tgcagcaagg ctggggccgc atcgtcagcg tcaccaccaa
     4741 gctggacacg atgaaccgga tcggcaccac gccatacggc gcatcgaaag cggcactgga
     4801 gatggcgacc gaagtgtggg cgaaggaact cgccggcacc ggcgtgacgg cgaatatcgt
     4861 caatccggga gccggggcga acacccccgg catggcgcag gagatgcgcg actggagccg
     4921 cgaaggcaaa gccccgcggc tggtcgaagc ggaggaaatg gtgccgccgc tgttgtacgt
     4981 cgcgtcggcg gaagcggatg cggtgaacgg ctaccggttc gatgcgaata cctgggacac
     5041 ggcgctgccg ccgaccgaag ccgcccgccg taacgcccgc cccgccgggt tcgtcttgca
     5101 tccgcaggat gcggccaatt ggcctggatg aaacggcggc acaacgcacg caggaatcag
     5161 tggcatgacg ttgcctctcg cgggaatccg cgtactcgac ctgtcctcca ttatcgccgc
     5221 acccgtgacc gccaccatgc ttggcgattt cggcgccgag gtggtgaagg tggaggatcc
     5281 gatccatggc gacttcatgc gccggggcgc ccgcgagccc ggcggacgct cgctgcaatg
     5341 ggtgcaggat gcccgtaaca aggagtcggt cacgctcaac ctgcggcacg ccgaagcgcg
     5401 tgccattgtg caccggctgc tgccgcattt cgacgtcgtg gtcaccaact tccgtccgcc
     5461 gactctgcgg cgctgggggt tcgatccgga taccgtgcgg gcccgtcatc cccgactgat
     5521 cgcgctctat gtcaccggct acgggctgac cggcccgtac agcgaccgtg gcgcgttcga
     5581 ccgcgtggcg agtgcctttg ccggactgac tcacgtcacc ggcgagtcgg accgcccgcc
     5641 ggtgcgatcc ggctacgcca cgatcgacta catgggcgcc tatgccggcg ccttcggggt
     5701 gatgacggcc ctctaccatc gcgattgccg cggcggcagc ggacaggtga tcgacctggc
     5761 actgtatgaa cccgggttcc gcgcctcgga agacgcgttg ctcgcctacg ccgcgacagg
     5821 acgcgtgcgg gagcgcagcg gcaacatcaa tccgaaagtg gtccccgcag gagatttcga
     5881 taccgccgac ggcaagcggg tgaccatcca cgccggcacg caaccgctgc tgcgccggct
     5941 ggccgagctg atgaacgaac ccggtctgtg cgacgatccg cggttcgcga cccatgcgcg
     6001 gcgggtggag aaccaggacg ccttgtacgc gacgatcgcc gactgggtca gccggcagaa
     6061 cctgacggac ctgatggcat cgctggtcgc ggcggacatc ccggcctccc cgctgatgag
     6121 cgtcgccgac atcgcggccg atccgcattt ccgtgagcgc ggcaccatca tgccggtgca
     6181 ggacgaggat cacggcgcgc tgctgatggc agcgcccatt ccccgcctga gcgagacccc
     6241 cggcagcgtg cgttcgctcg ggccgcggct gggttcggcg aacgagacga tcttcggcgg
     6301 tctgctggct atgagtgccg atgagatcgc ccgcctgcgg caggccggca ccatctgatc
     6361 gccttcactc cccccgctcc agcaggcacg catcgccgta ggagtagaac cggtatccgg
     6421 cctccaccgc gtgcgcatag gccgcctgca tccgggccgt cccggcgaag acgcagacca
     6481 gcatgaacag cgtcgagcgc ggcagatgga agttggtcag cagcaggtcg atggcgcgga
     6541 atcggtagcc gggcttgatg aacagctcgg tatcgccggc gaagggccgg atccgcccgc
     6601 cctcctccgc cgcggtctcc agcaggcgca cgctggtggt gcccaccgcg acaatgcggc
     6661 cgccggcctc ccgcgcggca ttgatcgcgg cggcggtgtc cgggccgatc tcgccgcgct
     6721 cagcgtggat gcgatgctgc gccaggtcgc cgcgaaccgg caggaaggtg ccggcgccga
     6781 catgcagggt gagcgtcgta cggccgatgc cgcgcgcctc cagcgcggcc agcaaggccg
     6841 gggtgaaatg cagcccggcg gtgggcgccg ccaccgcgcc ttcgcgtgcc gcgaacaccg
     6901 tctgatagtc gctggcgtcc tccggcaacg ggccgtgcgg gcgcgcgata tagggcggca
     6961 gcgccagcgc cccggcgcgc tgcagcgcct gcccgaaggc cgccccttcc aggttgaagc
     7021 gcagcgccac gccgccatcg ggatcgcgct gctgcacggt ggcggacaaa tcgtcggcac
     7081 cctcgaagcg cagcgtatcg cccgggtgca gccgccgggc attgcgcgcc agggcgtgcc
     7141 aggagccgtc cggcaagggc cggtccagcg tgattcccac ctgcgccgcg ccgcggcgag
     7201 cccgcagctg ggccgggatc accttggtgt cgttcgccac cagcaggtcg cccggccgca
     7261 gcaggtccgg caggtcccgc acgacatggt cgcccagccc gccggcggtg acatgcagca
     7321 gccgcgccgc ctcccgcggc cgcgccgggt gctgcgcaat ccgctctgct ggcagggcga
     7381 agtcgaacgc cgccatgccg tccggcacgc cggagcccgt cacgccgggg tcggcgccac
     7441 gcgccgcagc agccgcacca gcggcaccgc gatcagcact gcccatagct tgccgatgac
     7501 ctggccgggc aggaactcca ggctgccgaa ggcgagaatc aggaacacga tcgagtccac
     7561 caccagcccg accagcgacg aggccaccac cgcggtgacc agccgccgcc gctgcagcgg
     7621 ggtatagacc gcgaagtcgg ccagctcgct gagcgcgaag gcagtgccgg aggcgatcac
     7681 cagtgcgccg ggcgcgacca gggtggacag cgtggtgccc agggcgatcg ccgccagtcc
     7741 ccagtacagc cccaggcagc gttgcagcac gtcgcgcagc accagcgcgg cgccgacggt
     7801 cagcacgcca gacggcgcca ggataccggg agccaccggc accaggcagg gcccgtgcag
     7861 cgtacacacg gtgccgacgt gctggatcat ccagttggcg agcgggatgc tgcccaggaa
     7921 gcaggcgaag gcggcgaacc cgatcaggcg gcggcgatca ttctgcatca cgggctagta
     7981 cgaggcgatc tcgaccaggt tgccgtccgg atcgcggcaa tacaccgact tgatggcgcc
     8041 cagcgcaccg gcgcggttgc tggggccgat caggatggtg acgccgcaat cgtgcagatg
     8101 cgcgaccacc tcgtcgggtg gcaccgcggt gatgaagcag acatcgcccg atcccggcag
     8161 gtcgttcacc ccggtgggcc aggagccggg atcggcatcg gccgggcgca ggttgatctt
     8221 ctggccgcca aatttcagcg cggtgcggcg gtccgggccg aattcctcgc gttccatgcc
     8281 gagcacgcgc tggtaccagg ccgcggcgat ttccacgtcc ttcacgttca gcacgaggtg
     8341 gtcgatgcgg tcgatggtga agcgcatcgg tatggtcgcc tttgtggtgg gttgcgcggg
     8401 gaggaggtag ccgatttcgc cgacgctgca aatcggcggg ccgtctccct ggcgtctcgt
     8461 tgtacgtttc tcgtgacgtt ttcccgtggc atgagacggc gccggacgtg gcccaccacg
     8521 ccgggaccat gccgcagcgt gcggagtggg cggcatccgc gctgcgcttt cccggtcacg
     8581 catagcgccg cagccgttct tccgccgccg ggtccggcac gtccagctcc aacagcgacg
     8641 gcgtggtgcc gatgatgatc gcgcggagca gtttcggctc gggcggcccg aaatcgcagt
     8701 tgatcggcag gcggcccagt ctgcggatca gccgagcgcg gtcgggatac accttggcgt
     8761 agaattccgc ctcggcggcc cagtccggca ccggctcgtc gtcgtcgcgc ggccggacag
     8821 cattggattt cctgg
//
LOCUS       DASSZH010000640         2581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1686412, whole genome shotgun sequence.
ACCESSION   DASSZH010000640 DASSZH010000000
VERSION     DASSZH010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686412"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VFN42_13865"
     CDS             complement(<1..664)
                     /locus_tag="VFN42_13865"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HET8997750.1"
                     /translation="MPQVDGVFDVAVIGGGINGTGIARDAAGRGLSVVLLEQGDLGGA
                     TSSASTKLIHGGLRYLEHYAFRLVREALTEREVLLRAAPHLIRPMRFVLPHHPGLRPW
                     WMIRAGLFLYDHLGGRHILPGTRTLDLRRDPAGTPLQPRYTRAFEYSDCWTDDSRLVV
                     LNARDAAARGASILVRTRCTQARVVGGLWALTPDNGRALRARVLVNAAGPWVSQMLSE
                     VVH"
     gene            complement(666..1379)
                     /locus_tag="VFN42_13870"
     CDS             complement(666..1379)
                     /locus_tag="VFN42_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET8997751.1"
                     /translation="MIVADVMTRNVISVPPDATVEDAAKLMLQRRISGLFVVDAQGQL
                     AGVVTEGDLLRRDELGTQRHRPWWLRLLVSPGRQAADFTRTHGRRVRDVMTQDAITVA
                     SGAPLEDVVELMEQHRIKRLPVTQDGKVTGVVSRADLLRALVVASRKEPPVGDDDRSV
                     RERILNALDAESWAPTTSLNVTVLEGVVDLWGTITNDQERRAICVIAENTPGVKRVKD
                     HLVFIEPYSGTVIEAPEGC"
     gene            complement(1384..2190)
                     /gene="fetB"
                     /locus_tag="VFN42_13875"
     CDS             complement(1384..2190)
                     /gene="fetB"
                     /locus_tag="VFN42_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HET8997752.1"
                     /translation="MTPILLTPQDVAISAVLIVLDGALSLVLRLGLHRQLAIAATRMV
                     VQLVLIGFILRAVFAVQSPFATLCVVLVMVAIAGREVAARPEQKLGRFGNYAVGGGAV
                     LVATFLTAILALTTAIRPTPWYDPRYAIPLAGIVLGNVLNGASLALDSMLGGVVRERG
                     AIEAQLALGASFGQAIRPLVRASVRRALLPVINTMSAAGIITLPGIMTGQILAGMDPM
                     EAAKYQILLIFLLAGGSGLAAVAVVYLAARQLTDDRQRLRLDRLTAKSKG"
     gene            complement(2290..>2581)
                     /locus_tag="VFN42_13880"
     CDS             complement(2290..>2581)
                     /locus_tag="VFN42_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997753.1"
                     /translation="KFNLFLRIAARSLALRGEVIAVPGGRLGGLAGIVLMAAGTAMVA
                     LAAVRFVRTNRAIDSPEPGSDGARIDLLLAGLMVLLGLALVVYLSHTVTTGV"
BASE COUNT          430 a          893 c          907 g          351 t
ORIGIN      
        1 ggtgaaccac ctcggacagc atctgcgaca cccacggccc ggccgcattc accagcacgc
       61 gggcgcgcag tgccctgccg ttgtccgggg tcaatgccca cagcccgccg accactcgcg
      121 cctgggtgca gcgcgtgcga accaggatgc tggcgccgcg cgcggcggca tcccgggcgt
      181 tcagcacgac cagccgcgaa tcgtcggtcc agcagtcgga gtactcgaac gcccgggtgt
      241 agcgcggctg cagcggcgtg ccggcgggat cgcggcgcag gtccagcgtg cgcgtgccgg
      301 gcaggatgtg ccggccgccc aggtggtcgt agaggaacaa gccggctcgg atcatccacc
      361 acggacgcag cccgggatga tgcggcagca cgaagcgcat cggccggatc agatgcggcg
      421 cggcgcgcag cagcacctca cgctcggtca gcgcctcacg caccaggcgg aaggcgtagt
      481 gctccagata gcgcagcccg ccatggatca gcttggttga cgccgaggac gtcgcgccgc
      541 ccaggtcacc ttgttccagc agcaccaccg acaggccgcg gcccgccgca tcgcgcgcga
      601 tgccggttcc attgatgccg ccgccgatga cggcgacatc gaacacgccg tcgacctgcg
      661 gcatgtcagc agccctcggg cgcctcgatc accgtgccgg aatacggctc gatgaacacc
      721 agatggtcct tcacccgctt cacgcccggc gtgttctcgg cgatcacgca gatcgcccgg
      781 cgttcctggt cattggtgat ggtgccccac agatcgacca cgccctccag cacggtgacg
      841 ttcagcgatg tggtgggtgc ccaggactcc gcgtccaggg cattgaggat gcgttcccgc
      901 accgagcggt cgtcgtcccc gaccggcggc tccttgcggc tggccaccac cagggcgcgc
      961 agcaggtcgg cgcgcgacac cacgccggtg accttgccgt cctgcgtgac cggcaggcgc
     1021 ttgatgcggt gctgttccat cagctcgacc acgtcttcca acggcgcgcc ggaagcgacg
     1081 gtgatcgcgt cctgcgtcat cacgtcacgc acccggcggc cgtgcgtgcg ggtgaaatcg
     1141 gcggcctggc ggccggggga gaccagcagg cgcaaccacc acgggcggtg ccgctgggtg
     1201 ccgagttcgt cgcgccgcag caggtcgcct tcggtgacca cgccggccaa ctgaccctgt
     1261 gcgtccacca cgaacagccc ggaaatccgc cgctgcagca tcagcttggc ggcgtcttcc
     1321 acggtggcgt ccggcggcac cgagatcacg ttgcgggtca tgacgtcggc gacgatcatg
     1381 gcgttatcct ttgctcttgg cggtcagccg gtcgagacgc agccgctgcc ggtcgtcggt
     1441 cagctgtctg gcggcaagat acaccacggc aacggccgca aggccagaac cgccggccag
     1501 caggaagatc agcaggatct ggtatttcgc cgcctccatc gggtccatgc cggcgaggat
     1561 ctgtccggtc atgatgccag gcagggtgat gatgccggcg gcggacatgg tgttgatcac
     1621 cggcagcagc gcgcgccgca ccgaggcgcg gaccagaggg cggatcgcct ggccgaagct
     1681 ggcgcccagc gcgagctgcg cctcgatcgc gccacgctcg cgcaccacgc cgccgagcat
     1741 ggaatcgagc gccagtgaag cgccgttcag cacgttgccc agcacgatgc cggcgagcgg
     1801 gatggcatag cgcgggtcgt accacggcgt cgggcggatc gcggtggtca gcgccaggat
     1861 ggcggtgagg aaggtggcca ccagcaccgc gccgccgccc accgcgtaat tgccgaagcg
     1921 gccgagtttc tgctcgggcc gcgcggcgac ttcgcggccg gcgatggcga ccatcaccag
     1981 caccacgcat agcgtggcga agggcgactg cacggcaaac accgcgcgca ggatgaagcc
     2041 gatcagcaca agctgcacca ccatgcgggt cgcggcgatg gcgagctggc ggtgcaggcc
     2101 gagccgcagg accagcgaca gggcgccgtc cagcacgatc agcacggcgg agatcgcgac
     2161 gtcctgcggg gtgagaagga tgggggtcat ggctggcagg tggtcacgaa cagcgcgaac
     2221 cccattcctg gtgtttgtcc gccggaggca gcgccgagtc cgcacgcgtc cgggtgggct
     2281 gtgcggacgt cagaccccgg tcgtcacggt atgcgagagg taaaccacga gagcgaggcc
     2341 gagcagcacc atcagccctg ccagcagcag gtcgattcgg gcgccgtcac tgcctggctc
     2401 cgggctgtcg atggcgcgat tggtccgcac gaaacgcact gcggccagag ccaccatggc
     2461 ggtgccggcg gccatcagca cgattccggc gaggccgccc agacggccgc cgggtaccgc
     2521 gatgacctcg ccgcgcaatg ccagtgagcg ggcagcgatg cgcaggaaca ggttgaactt
     2581 g
//
LOCUS       DASSZH010000641         6432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_595288, whole genome shotgun sequence.
ACCESSION   DASSZH010000641 DASSZH010000000
VERSION     DASSZH010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6432
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595288"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1831
                     /locus_tag="VFN42_13885"
     CDS             <1..1831
                     /locus_tag="VFN42_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET8997754.1"
                     /translation="LKQQMEAERIDTKRWTPALLMATIQRWKDRGLTPARITPAEDSD
                     FANGRAKGLYAAYQERLRALNAADFGDLLLHMTEILRGHPDVLADYHRRFRYILVDEY
                     QDTNLIQYLWLRLLAQGHHNICCVGDDDQSIYSWRGAEVENILRFEKDFPGAKIVRLE
                     SNYRSTAPILAAASALIAHNEGRLGKTLRTGRQDGQHGEKVTVVGLWDSDEEARMVGE
                     RIEALRREGHPLTEIAILVRAGFQTRAFEERLITLGIPYRVVGGLRFYERAEIRDAIA
                     YMRVLVQPADDLAFERIVNVPRRGVGDAALRALHATARSETLPLSGAAARAIESGAVR
                     GKVKEALATLLQGFARWRKLLETEGHVVALATLLDESGYTAMWQQDKSAEAPGRLENL
                     KELVRALAEFETLQGFLDHVALVVENEETANGDRVNLMTLHGAKGLEFDTVFLPGWEE
                     GVFPNQRAMDESGNKGLEEERRLAYVGLTRARRRAIVSHAANRRIYANWQTSIPSRFL
                     DELPDANVERTGSAALARDARIAAATSFSGQFPLVARRPRVIEAWEQPARPPREDAVP
                     VGARVFHQKFGYGTVTATEDGRLDVAFDKAGQKRVLDRFVERA"
     gene            complement(1889..2356)
                     /locus_tag="VFN42_13890"
     CDS             complement(1889..2356)
                     /locus_tag="VFN42_13890"
                     /inference="COORDINATES: protein motif:HMM:NF019028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone H1-like repetitive region-containing
                     protein"
                     /protein_id="HET8997755.1"
                     /translation="MSNEETDKPRAQAMAMRAGTRKAAAKKGGRKAAAKKGTRKVAAK
                     KTARKTAARKTTTRKTAAKKGARKTATRGRKTAAKKTARKAAGRKTATRKTAARKTTR
                     KTAARKTTRKTTTRKTAARKSTPRKTATRRPRKSAAPEMAPEMSGEGSNDSME"
     gene            complement(3269..4423)
                     /gene="prpC"
                     /locus_tag="VFN42_13895"
     CDS             complement(3269..4423)
                     /gene="prpC"
                     /locus_tag="VFN42_13895"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HET8997756.1"
                     /translation="MSDTANTPNRAKRGVGLSGVVAGNTALCTVGRSGNDLHYRGYDI
                     LDVADTCEFEEIAHLLVHGALPTRAELTAYKAKLRSMRGLPLAVKQAMEALPAASHPM
                     DVMRTAVSALGCTLPEKDDQNQPGARDIIDRLMACLGSALLYWFHYAHNGKRIDVETD
                     DDSIGGHFLHLLHGKPPSEMFVRAMHTSLILYAEHEFNASTFTCRVVAGTGADMHSAI
                     TAGIGALRGPKHGGANEVSFEIQNRYANPDEAEADIRKRVAERQVIIGFGHAVYTVAD
                     PRNKVIKEVARRLSQAAGSMKLFNIADRIEATMWDAKQMFANLDWFSAISYHMLGVPT
                     AMFTPLFVIARTSGWGAHVIEQRIDNKLIRPTANYTGPENLKFVPIDQRG"
     gene            complement(4598..6097)
                     /locus_tag="VFN42_13900"
     CDS             complement(4598..6097)
                     /locus_tag="VFN42_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HET8997757.1"
                     /translation="MILHDVRVHPSKDNLPREAQLAWKIAAVAADKVAVEREVTEMII
                     NRIIDNASVAIASVNRHPIISARDAAIGHPRKDGATIFGMPHSKGFSPEWAAWANGTA
                     VRELDMHDTFLAADYSHPGDNIPPVLAVAQSMEKSGRDLIRGIATGYEIHIDLVRAIC
                     LHAHKIDHIMHLCPAQAAGIGALLNLKAEVIYQAVQQAVHVSVTTRQSRKGEISSWKA
                     YAPAHAGKLAIEAVDRCMRGEGAPSPIYEGEDSVIAWVLDGPEAQYQVPLPAPGEPKR
                     AILDSYTKEHSAEYQSQALIDLAFRMREQIADLEKIEKIVIHTSHHTHYVIGTGANDP
                     QKMDPKASRETLDHSIMYIFAVALQDGRWHHVDSYAPRRAGRADTVRLWHKIETREEP
                     EWTRRYRSTDPNELAFGGRVEITMQDGSVITDELAMANAHPLGARPFARDDYIGKFRT
                     MTDGIISTREANRFLDVVQDLPRLAAGELHLLNVALPAGTLKQGKAGIF"
BASE COUNT          963 a         2132 c         2217 g         1120 t
ORIGIN      
        1 gctgaagcag cagatggagg cagagcgcat cgacaccaag cgctggaccc ctgccctgct
       61 gatggcgacg atccagcgct ggaaggaccg cggcctgacg ccggcgcgca tcaccccggc
      121 ggaggacagc gacttcgcca atggcagagc caagggcttg tacgccgcct accaggagcg
      181 gctgcgtgcg ctgaacgctg ccgatttcgg cgacctgctg ctgcacatga cggagatcct
      241 gcgcgggcat cccgatgtgc tggccgacta ccaccgccgc ttccgctaca tcctggtcga
      301 cgagtaccag gacaccaacc tgatccagta cctctggctg cgcctgctgg cgcagggtca
      361 ccacaacatc tgctgcgtgg gggacgacga ccagtcgatc tactcgtggc gcggcgccga
      421 ggtggagaac atcctccgct tcgagaagga cttccccggc gcgaagatcg tccggctcga
      481 gtcgaactac cgctccaccg cgccgatcct ggccgccgcc tcggcgctga tcgcgcacaa
      541 tgaaggccgg ctgggcaaga cgctgcgcac cggccggcag gacggacagc acggcgagaa
      601 ggtgaccgtc gtggggctgt gggattccga cgaggaagcc cgcatggtcg gcgaacgcat
      661 cgaagcactg cggcgcgagg ggcatccttt gacggagatc gctatcctgg tgcgcgccgg
      721 tttccagacc cgcgccttcg aggaacggct gatcaccctg ggcattccct atcgcgtggt
      781 cggcggcctg cgcttctacg agcgcgcgga gatccgcgac gccatcgcct atatgcgcgt
      841 gctggtgcag ccggccgacg acctggcatt cgaacgcatc gtcaacgtgc cccgccgcgg
      901 cgtgggtgac gcggcgctgc gcgcgctgca cgccacggcg cgaagcgaga ccctgccgct
      961 atcaggcgcc gccgctcgcg cgatcgagag cggtgcggtc aggggcaagg tgaaggaagc
     1021 gctcgccacg ctgctgcaag gcttcgcgcg ctggcgcaag ctgctggaga cggagggcca
     1081 tgtggtcgcg ctcgccacgc tgctggacga gagcggctac acggcgatgt ggcagcagga
     1141 caaatcggcg gaggcgccag gccggctgga gaacctgaag gagctggtgc gcgcgctggc
     1201 ggaattcgag acgctgcagg gcttcctcga ccacgttgcg ctggtggtgg agaacgagga
     1261 gaccgccaac ggcgaccggg tgaacctgat gacgctgcac ggcgccaagg gactggagtt
     1321 cgacacggtg ttcctgcccg gctgggagga aggcgtgttc cccaaccagc gtgcgatgga
     1381 tgaatccggc aacaaaggcc tggaggaaga acgccggctg gcctatgtcg gtctgacccg
     1441 cgcgcgccgc cgcgccatcg tcagccacgc ggcgaaccgg cgcatctacg ccaactggca
     1501 gaccagcatc ccaagccgct tcctcgacga actgccggat gccaatgttg agcgcacggg
     1561 ttctgccgca ctggcacgcg acgcccgcat cgccgcagcc acgagctttt ccgggcagtt
     1621 cccgttggtg gctcgccgtc cgcgggtgat cgaggcctgg gaacagccgg cacgtccgcc
     1681 gcgtgaagac gcggtgccgg tcggtgcgcg ggtgtttcac cagaagttcg gctacggcac
     1741 ggtcaccgcc accgaagacg gcaggctgga tgtggccttc gacaaagccg gccagaagcg
     1801 cgttctggac cgcttcgtgg aacgcgcctg acaaagaaac gccctctccg cccggcaggg
     1861 cggagagggc gaacgtggca agcgcaggct attccatcga gtcgttggag ccttcgcccg
     1921 acatttccgg agccatctcg ggcgcggcgg acttgcgcgg acgccgcgtg gcggtcttgc
     1981 gcggggtgct cttgcgcgcc gcggtcttcc gcgtggtggt cttgcgcgtg gtcttgcgcg
     2041 ctgcggtctt ccgcgtggtc ttgcgcgccg cggtcttgcg ggtggcggtc ttccggccgg
     2101 ccgccttgcg tgccgtcttc ttcgcggcgg tcttgcggcc gcgcgttgcg gtcttccggg
     2161 cgcccttctt tgccgcggtc ttgcgcgtgg ttgtcttgcg cgccgcggtc ttgcgggcgg
     2221 tcttcttcgc cgcaaccttg cgggtgccct tcttggcagc ggccttgcgc ccgcccttct
     2281 tcgccgcggc cttgcgcgtg ccggcccgca tcgccatggc ctgggcgcgc ggcttgtcgg
     2341 tttcttcgtt gctcacctga tgaagctcct tttggtgtcg ttggttgccg acgcgcggta
     2401 cacgggtggc gttgcacagc ccccggctcc gcgcgatgtc cgcggcggaa cgacgttccc
     2461 gaatgcggac cagtaacagc gggacagcga tcggaccggc cttgcctcgc cagtcccgcg
     2521 cgcgccaatg gcggcgccgc gggcagcgaa cttccgactc tcacaggggc ggcgaaccaa
     2581 gccggcgctg aacagcgccg tggtctccgt cgccatagcc gcgatctgct ccacttcgtc
     2641 ggcgcaggat cgagtctgct tcgcctggac gaggctgcgg tccatggacc gtccttctgc
     2701 ttgcatgtgc gcgttgcaac cgatcgacag atcagtgcga ctcggaatcg cgcacatcag
     2761 attcgctatt ccgcgtgtcg cccgcgcgtg tcaaacaaaa acaacacgcg aatcgcaggc
     2821 acgatgaaaa attccggcgt ggcgtctcat gcgatgcatc gccgccacac attgcgcgct
     2881 gcagaggagg gttggcgatg caaaccacct cgtgtcgaaa gcctcgcgcg cgcaatcagg
     2941 cagcaggaac gcgcgccgtg cgcagcgcat cgtggcggca aggcggacga tgcaaaccac
     3001 gtcgtcgcgg gcagaggccg gcgatgcaag ccacgtcgtg gcggcgaagg ccggcgatgc
     3061 aagccgcgtc gtcgtggcga agacccgcca cccacgactg cctgctggac actcgaagcc
     3121 cgaaacccgc gctttgcagc ttgaaacttt cggcaagacg tgggtgcccg ggccaagccc
     3181 gggcatgaca cagcgggggg aaccgccaaa cagaaaccgc cgcggcatcg ctgccgcggc
     3241 ggtctgcatc cggcgccctg gcgcggtatc agccgcgctg gtcgatcggc acgaacttca
     3301 ggttttccgg cccggtgtag ttcgccgtcg ggcggatcag cttgttgtcg atgcgctgtt
     3361 cgatgacgtg ggcgccccag ccggaggtgc gggcaatcac gaaaagcggc gtaaacatcg
     3421 ccgtcggcac gccgagcatg tggtaggaga tggcgctgaa ccagtcgagg ttggcgaaca
     3481 tctgcttggc gtcccacatc gtcgcctcga tccggtcggc gatgttgaac agcttcatgc
     3541 tgcccgccgc ctgcgacagc cggcgcgcca cctccttgat caccttgttg cgcggatcgg
     3601 ccaccgtata gacggcgtgg ccgaagccga tgatcacctg ccgctccgcc acgcgcttgc
     3661 ggatatcggc ttccgcctcg tccgggttgg cgtagcggtt ctggatctcg aaggagacct
     3721 cgttggcgcc gccatgcttc ggcccgcgca gcgcgccgat gccggcggtg atcgccgaat
     3781 gcatgtccgc cccggtaccc gccaccacgc ggcaggtgaa agtcgaggcg ttgaactcgt
     3841 gctcggcgta gaggatcagg ctggtgtgca tcgcccggac gaacatctcg ctgggcggct
     3901 tcccgtgcag caggtgcagg aagtggccgc cgatcgaatc gtcgtcggtc tccacgtcga
     3961 tccgcttgcc gttatgggca tagtggaacc agtagagcag cgcggagccg aggcaggcca
     4021 tcagccggtc gatgatgtcg cgggcgcccg gctggttctg atcgtccttc tccggcagcg
     4081 tgcagcccag cgccgagacg gcggtgcgca tcacgtccat cgggtgcgac gcggccggca
     4141 gcgcctccat cgcctgcttc accgccagcg gcagtccgcg catcgagcgc agcttggcct
     4201 tgtaggcggt gagctcggcg cgggtgggca gcgcgccatg caccagcagg tgggcgatct
     4261 cctcgaactc gcaggtgtcg gcgacgtcca ggatgtcgta gccgcggtag tgcaggtcgt
     4321 tgccgctgcg gccgacggtg cacagcgcgg tgttgccggc gacgacgccg gacaggccga
     4381 cgccacgctt ggcgcggttg ggagtgttgg ctgtgtcgct catgtgggtg ctcctttgtg
     4441 gtccatgcgg cgtggcggtg tgtgcgatgg cccctccgtg tcatggccgg cgaaggccgg
     4501 ccacccatgt ctttgccgta ttcccctcag caagacgtgg gtggccgggc caggcccggc
     4561 catgacacga agcggccaca gcactggggt tcggtgttca gaaaatccct gccttgcctt
     4621 gcttcagcgt gccggccggc agtgcgacgt tcaacagatg cagctcgccg gcagcgaggc
     4681 gcggcaggtc ctgcaccacg tccaggaagc ggttcgcctc ccgcgtgctg atgatcccgt
     4741 cggtcatggt gcggaacttg ccgatgtagt cgtcccgcgc gaagggccgc gcgcccagcg
     4801 gatgcgcgtt ggccatcgcc agctcgtcgg tgatcacgct gccgtcctgc atggtgatct
     4861 ccacccggcc gccgaacgcc agctcgttcg gatcggtgga gcgatagcgc cgcgtccatt
     4921 ccggctcctc gcgggtttcg atcttatgcc acagccgaac cgtatccgcc ctgcccgccc
     4981 gcctgggcgc gtagctgtcg acgtggtgcc agcgcccgtc ctgcagtgcc acggcgaata
     5041 tgtacatgat cgagtgatcg agcgtctccc ggctcgcctt ggggtccatc ttctgcgggt
     5101 cgttggcgcc ggtgccgatc acgtaatgcg tgtggtggct ggtgtggatg acgatcttct
     5161 cgatcttctc caggtcggcg atctgctccc gcatgcggaa ggccaggtcg atcagcgcct
     5221 gcgactggta ttccgcgctg tgctccttgg tgtagctgtc caggatcgcc cgcttgggtt
     5281 cgccgggcgc cggcagcggc acctggtact gcgcctccgg tccgtccagc acccaggcaa
     5341 tcacgctgtc ctcgccttca tagatcgggc tgggcgcacc ctcgccgcgc atgcagcgat
     5401 ccaccgcctc gatcgccagc ttgccggcgt gcgccggtgc ataggccttc caggagctga
     5461 tctcgccttt gcgtgactgg cgggtggtga cgctgacatg caccgcctgc tgcaccgcct
     5521 ggtagatcac ctcggccttc aggttcagca gcgcgccgat cccggccgcc tgcgccgggc
     5581 agagatgcat gatgtggtcg atcttgtgcg cgtgcaggca gatggcgcgc accaggtcga
     5641 tatggatttc atagccggtg gcgatgccgc ggatcaggtc gcggccggac ttctccatgc
     5701 tctgcgccac ggcgagcacc ggcgggatgt tgtcgccggg atgcgagtaa tcggcggcga
     5761 ggaaggtgtc gtgcatgtcc agctcgcgca ccgcggtgcc gttcgcccag gcggcccatt
     5821 ccgggctgaa ccccttgctg tgcggcatgc cgaagatcgt cgcgccgtcc ttgcgcggat
     5881 ggccgatcgc ggcatcccgc gcagagatga tcgggtggcg gttcaccgag gcgatcgcca
     5941 ccgacgcgtt gtcgatgatg cggttgatga tcatctcggt gacttcgcgc tccaccgcca
     6001 ccttgtcggc ggcgacggcg gcgatcttcc aggcgagttg cgcctcgcgc ggcagattgt
     6061 ccttggatgg gtgaaccctg acgtcgtgca gtatcatgcc ttcctccgct gggcgcggag
     6121 cggcagaggg ctgccgaaac cgcgcggtcc tggccttgcc cgatcttcgt aagagtgtgt
     6181 cgccgtaacg gcaagcggag ggggcgaagg cagcggagcg tacagatata taagtgagcg
     6241 atcgcgccat gccgggcgag gctgccggcg tccgggccga accggttttc cgcgcagcgc
     6301 tcgcttcggc tagcatgggc cgccttctcc gagggcatgc cgcagcaatg gagcaccgct
     6361 cgcatgacca acgccgttcc gttcgatcgg gtcgtccgcg gcaccgtggt gaccgccgac
     6421 accgtgctgc ct
//
LOCUS       DASSZH010000642         3561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1191701, whole genome shotgun sequence.
ACCESSION   DASSZH010000642 DASSZH010000000
VERSION     DASSZH010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191701"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..998
                     /gene="fabI"
                     /locus_tag="VFN42_13905"
     CDS             156..998
                     /gene="fabI"
                     /locus_tag="VFN42_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019091259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HET8997758.1"
                     /translation="MPESAPPGLPVKGTIMQGRRGLIMGVANDRSLAWGIAAACAAQG
                     AELAFTFQGEALERRVRPLAASVGSSLVFPCDVADEASIDAAFAALREHWDGLDFLVH
                     AIGYADKQYLRGRFLDTPRGAFLQALDISCYSFVAVGQRAVPMMRPGGSLLTLSYMGA
                     ERWTPHYNVMGVAKAALEASVRYMAADLGGAGIRVNAISAGPIKTLAASGIGDFRYIL
                     RWNQLNSPMQRNVTIEEVGGAGVYLLSDLGSGVTGEVHHVDCGYHIVGMKNPAAPDIA
                     IVEG"
     gene            999..1550
                     /locus_tag="VFN42_13910"
     CDS             999..1550
                     /locus_tag="VFN42_13910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET8997759.1"
                     /translation="MPAITADSLLALMQEKGSRRYGLHDVSQLQHALQAATLAEQEGC
                     PPALIVAALLHDIGHMVHALGDNPAAEGVDDRHEAVGHAYLAARFGPEVTEPVRLHVA
                     AKRYLCATEPDYFGRLSPDSVLSLSLQGGPMSDAEVAAFRALPHAAAAVRLRRYDEAA
                     KVKDLPTPPLAHFRPHLDACLAA"
     gene            1641..2156
                     /locus_tag="VFN42_13915"
     CDS             1641..2156
                     /locus_tag="VFN42_13915"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HET8997760.1"
                     /translation="MTSRPLLVALALGASLAAMPALAATAPNQPNSSSPNHSEQAVAK
                     DSNHSGANNGAWKTAASDKTPDKLNPLLTSKGLVQMKKLIGSDVYNDQDKKLGSIDDV
                     LVDKHGQPTVLVRVNSKLHEVPWSKLRFGDAEKSDHNKVLMPDESKDALNATPQFQDK
                     GKDTGKKKSSG"
     gene            complement(2401..3540)
                     /locus_tag="VFN42_13920"
     CDS             complement(2401..3540)
                     /locus_tag="VFN42_13920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8997761.1"
                     /translation="MKVGTALNMLCQPGRSDASVVHEHMAMGDLVEPLGFDSLFALEH
                     HFTGYAMSPAPLQLLAYYAGRTRRIQLGTAVIVLPWQDPIRVAEQIALLDIMSGGRCL
                     FGFGRGAASVEYEGFRIPMGEARPRFIEAAEIIQRALSQDSFDYQGQFFQIPRTAIRP
                     RPISHPERRFYGSAVSPDSAEVMAKLGFSLLVIMQNEWPKAATDVHRFREMSVSYGHT
                     PKPPIILTNVSCAESRAEAQERALEYLGRKWDSIDEHYHFSDGHLASVKGYESYEKVG
                     VTYAKMKEPARRAKATDFYVSIQVVGTPDDCLQKIAELQRLTGMDHLVTEFSFGGLPH
                     HEAELGMRLFADKVLPVLQRDALFAAAPPTEVIAQEAGEGVFAPA"
BASE COUNT          605 a         1157 c         1228 g          571 t
ORIGIN      
        1 atggtggggg aacccaccgc ctgatcctgc ggcctgccaa accggtgccc ctgccacgca
       61 ccggcgcccg tgcccaccgg tcccaccctt gccgggcgcc gcttgcctgt gccaccaacg
      121 ccacaggact ccgcaacaag ccctagagga tctgcatgcc tgagagcgct ccccccggcc
      181 tgcccgtgaa gggcacgatc atgcagggtc ggcgcggcct gatcatggga gtggcgaacg
      241 atcgctcgct ggcctggggc atcgcggccg cctgtgcggc gcaaggcgcc gaactcgcct
      301 tcaccttcca gggcgaggcg ctggagcggc gggtgcgccc gctggcggcc tcggtgggat
      361 catccctggt gttcccctgc gacgtggcgg atgaagccag catcgacgcc gccttcgccg
      421 cgctgcgcga gcactgggac gggctggatt tcctggtgca tgcgatcggc tatgccgaca
      481 agcagtatct gcgcgggcgt ttcctggaca cgccgcgcgg cgcgttcctg caggcgctgg
      541 atatctcctg ctactccttc gtcgcggtcg gccagcgcgc ggtgccgatg atgcgcccgg
      601 gcgggtcgct gctgacgctg agctacatgg gcgcggaacg ctggaccccg cactacaacg
      661 tgatgggtgt ggccaaggcg gcgctggagg cgtcggtgcg ctacatggcc gccgacctgg
      721 gcggcgccgg catccgggtg aacgcgatca gcgccgggcc gatcaagacc ctggcggcca
      781 gcggcatcgg cgacttccgc tacattctgc gctggaacca gctcaattcg ccgatgcaac
      841 gtaacgtcac catcgaggaa gtcggcggcg ccggggtgta tctgctgtcc gacctcgggt
      901 cgggcgtcac cggcgaggtg catcatgtcg attgcggcta ccacatcgtc ggcatgaaga
      961 acccggcggc gccggacata gccatcgtcg agggatgaat gcccgcgatc accgccgact
     1021 cgctgctggc gctgatgcag gagaaaggca gccgccgcta cgggctgcat gacgtcagcc
     1081 agttgcagca cgcgctgcag gcggcgacgc tggcggagca ggagggttgc ccgccggccc
     1141 tgatcgtcgc cgcgctgctg cacgacatcg gccacatggt gcacgcgctg ggcgacaatc
     1201 cggcggccga gggcgtcgat gaccggcatg aagccgtggg ccatgcgtat ctggcggcgc
     1261 ggttcggccc ggaggtgacc gaaccggtgc ggctgcacgt cgcggcgaaa cgctatttgt
     1321 gcgcgaccga accggattat tttggccgcc tgtcgccgga cagcgtgctg agcctgtcgc
     1381 tgcagggtgg cccgatgtcg gatgccgagg tcgcggcatt ccgcgcgctg ccacacgccg
     1441 cggccgcggt gcggctgcgg cgttacgacg aggcggcgaa ggtgaaggac ctgccgacgc
     1501 cgccgctggc gcatttccgg ccgcacctgg atgcctgcct ggccgcgtaa cggtgcccgg
     1561 ctcgcttcat aaccgcgtgc ggccatcgcg ctgcaacggc gcgcaccgcg ttctcccggc
     1621 acaggcaagg gagatttcgc atgaccagca ggccgctcct cgttgcgctc gcgctgggcg
     1681 ccagtctcgc ggcgatgccc gcgctggcgg cgacggcgcc caatcagccg aattcatcca
     1741 gccccaatca tagcgagcag gcggtcgcaa aggacagcaa tcactccggc gcgaataacg
     1801 gcgcgtggaa gaccgcggcg tcggacaaaa cgcccgacaa gctgaacccg ctgctgacca
     1861 gcaaggggct ggtgcagatg aagaagctga tcggcagcga tgtgtacaac gaccaggaca
     1921 agaagctggg cagcatcgac gacgtgctgg tggacaagca tggccagccc acggtcctgg
     1981 tgcgcgtcaa cagcaaattg cacgaggttc cctggagcaa gctgcgcttc ggcgacgcgg
     2041 agaagagcga ccacaacaag gtgctgatgc cggatgagag caaggacgcg ctgaacgcca
     2101 caccgcagtt ccaggacaag ggaaaggaca cgggcaagaa gaagagcagc ggctgaggcg
     2161 gtagatcgag ccgactgaag ccaccgccac gaggttccgc ggcgagtcgc gggtgagtcg
     2221 cgggtgggtc gcaggttgac tgcatacccg tcgtggcggg cgaaggcccg ccacccacga
     2281 cttgttggcg aagacgcatt atcgcaagcc gcttctgcga gtcttccaca gccattcgcg
     2341 ggtggcgggc ctgcgcccgc cacgacgatg tacggcagtc tgggggctca tcttcgcagg
     2401 tcaggcggga gcgaacacgc cttccccggc ttcctgcgcg atcacctccg tcggcggtgc
     2461 agcggcgaac agcgcatcgc gctgcagcac cgggagtacc ttgtcggcga acagccgcat
     2521 gccgagctcc gcttcgtggt gcggcaggcc accgaaggag aactcggtga ccaagtgatc
     2581 catgccggtc agccgctgca gctccgcgat tttctgcagg cagtcatccg gtgtgcccac
     2641 cacctggatg ctgacataga agtcggtggc tttcgcccgg cgcgccggtt ccttcatctt
     2701 cgcataggtg acgccgacct tctcgtacga ttcgtaaccc ttcaccgacg ccaggtggcc
     2761 atcggagaaa tggtagtgct cgtcgatgga atcccatttg cggccgaggt attccaacgc
     2821 gcgctcctgc gcctcagctc tgctttcagc acaggagaca ttggtcagga tgatcggcgg
     2881 tttcggcgta tggccgtacg ataccgacat ctcgcggaag cggtgcacgt cggttgccgc
     2941 cttcggccat tcgttctgca tgatcaccag cagcgagaag ccgagcttcg ccatcacctc
     3001 ggcggagtcg gggctgacgg cggagccgta gaagcggcgt tccggatggg agatcgggcg
     3061 cggacggatc gccgtgcgcg ggatctggaa gaactggccc tggtagtcga aactgtcctg
     3121 cgacagtgca cgctggatga tctccgccgc ctcgatgaag cgcggccggg cctcgcccat
     3181 cgggatgcgg aagccttcgt attccacgct cgcggcgccg cggccgaacc cgaacaggca
     3241 gcgcccgccg gacatgatgt cgagcagggc gatttgttcg gcgacgcgga tcgggtcttg
     3301 ccagggcagc acgatcaccg cggtgcccag ttggatgcgc cgcgtgcgtc cggcgtagta
     3361 ggcgagcaac tgcagcggtg ccggcgacat cgcgtagccg gtgaaatggt gttccagcgc
     3421 gaacagggaa tcgaagccga gcggttcgac aaggtccccc atggccatgt gttcgtgtac
     3481 cacggaggca tcggaccggc ctggctggca cagcatgttg agcgccgttc cgactttcat
     3541 ggctggtttc ctccggcttt a
//
LOCUS       DASSZH010000643         3217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1532963, whole genome shotgun sequence.
ACCESSION   DASSZH010000643 DASSZH010000000
VERSION     DASSZH010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532963"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1851)
                     /locus_tag="VFN42_13925"
     CDS             complement(<1..1851)
                     /locus_tag="VFN42_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997762.1"
                     /translation="MKTGVLGAIGAEDLRRPAEVNAALAANDRVKYAFSLLQMAAAHA
                     DQPQLPAATMQRERLACGIDDAALDRFTASASRRGGIYLIPGAGRLMGQIVADMRLMA
                     APVLDSGQPEFASRLDALLGAMPAAGDDRLDPAGLAAITQAADGADSLHRLVMDLHKA
                     LNAMQAALAEEHIDGAAAYGLADTDRPRVAAFMAGLNRTAKLKFDHPGLGTTATRSGN
                     RLVIQNDIGTTDAHVIVVHVEDRTITVTYTDVHPERLRFFQGMLARCAPDWETTRAQH
                     LSETDFQLATGRYIAPDEAACRDSLSFLGSRLVFLIDWNRARRQLRGFLPGDARIELL
                     RWAAEQEVGHRAFLELGGARLVNQAIEAVAGSAMHFGDRLSDVLGLAETQSFLRFVLR
                     AAADCLLSRRSPSLLRDRIRAELARHFSNEERRLLRLAADHAALGFELACLVRDAALA
                     PEEIDADRLIGRARGFEHDADRLVADTFEAVKRRPDYAVFGPLLHTADNCADALEDAV
                     FLLGLLPAAGSAGPVLQPLQALTGLLVDVCQEWLKALLHAAHVEDRSAAGDADDFLVA
                     IDRIGALEHQVDDAERSLIAAAVQHAADFRQLHLYSALGARLEEAADAVRG"
     gene            complement(1941..2432)
                     /locus_tag="VFN42_13930"
     CDS             complement(1941..2432)
                     /locus_tag="VFN42_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997763.1"
                     /translation="MAKCLLLPAALLLIAAAPPRDLPNPHRTPGAIDPAITQATIDRT
                     ICSRHWSTRDIRPPEDYTYRLKRYQLRAWGYRDRRTRDYEEDHLISLELGGAPSSPRN
                     LWPEPWRGHCGAHAKDRVENALHRAVCDGELSLRAAQQAIATDWVAAYRDYVGPIQCR
                     TGR"
     gene            2608..>3217
                     /locus_tag="VFN42_13935"
     CDS             2608..>3217
                     /locus_tag="VFN42_13935"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8997764.1"
                     /translation="MLTTNDFRLDGRVALVTGSGRNIGRAIAEAYAAAGARVVLNGHR
                     DAAALEEVAQGIRARGGEALPILADVADDAAVGRMVDQAVATFGSVDVVVSNVGIRKY
                     RAFLEITPEEWDAVLRTNLSASFYLARHAIPHMRTRNWGRVIVISGADGFWGHVTHRA
                     PNLAAKSGMHGLAKAIAREFGADGITANTVSPGPIDTVRDWSQ"
BASE COUNT          500 a         1093 c         1134 g          490 t
ORIGIN      
        1 gccgcgcacc gcgtcggcgg cttcctccag ccgcgcgcca agcgcggaat acagatgcaa
       61 ttggcggaaa tccgctgcgt gctgcacggc ggctgcgatc aggctgcgct ctgcatcgtc
      121 cacctggtgc tccagcgcgc cgatccggtc gatcgccacc aggaaatcgt cggcatcgcc
      181 cgccgcgctg cggtcctcca catgcgcagc atgcagcagc gccttcagcc attcctggca
      241 gacgtccacc agcagcccgg tcagcgcctg cagcggttgc agcaccggcc cggcggagcc
      301 ggcggcgggg agcagcccga gcaggaacac cgcatcctcc agcgcatcgg cgcagttgtc
      361 ggcggtgtgc agcaatgggc caaagaccgc atagtcggga cggcgcttga ccgcctcgaa
      421 tgtgtcggca accaggcggt ccgcatcgtg ttcgaagccg cgggcgcggc cgatcagacg
      481 atcggcgtcg atctcctccg gcgccagggc cgcgtcgcgg accagacatg ccagttcgaa
      541 ccccagcgcc gcgtggtcgg cggccagccg cagcaggcgg cgttcctcat tgctgaagtg
      601 gcgggcgagc tccgcccgga tgcggtcgcg cagcaaggag ggtgaacggc gcgacaacag
      661 gcagtcggcc gcagcgcgca gcacgaagcg caggaagctt tgcgtctcgg cgaggccgag
      721 cacgtcggac aggcggtcgc cgaaatgcat ggcggagccg gcaacggcct cgatcgcctg
      781 gttcaccagc cgggcgccgc cgagttccag gaaggcgcga tggcctacct cctgctccgc
      841 cgcccatcgc agcagttcga tgcgggcgtc gccgggcagg aacccgcgca actgcctgcg
      901 ggcgcggttc caatcgatca ggaacaccag gcgcgagccg aggaagctga gactgtcgcg
      961 gcacgccgcc tcgtccggcg cgatgtagcg tccggtggca agctggaaat cggtctcgga
     1021 caggtgttgg gcgcgggtgg tttcccagtc cggcgcgcag cgggccagca tgccctggaa
     1081 gaagcgcagg cgctccggat gcacgtcggt gtaggtgacg gtgatcgtgc gatcttccac
     1141 atgcacgacg atcacgtgcg catccgtggt gccgatgtcg ttctggatca ccagtcggtt
     1201 gccgctgcgg gtcgcggtgg tacccaggcc ggggtggtcg aacttcagct tcgccgtgcg
     1261 gttcagcccg gccatgaagg cagctacccg cggccggtcg gtgtcggcca ggccgtatgc
     1321 ggcggcgccg tcgatgtgtt cctccgccag cgccgcctgc atggcgttca gcgccttgtg
     1381 caggtccatc accagccggt gcaggctgtc ggcgccatcc gccgcctggg tgatggcggc
     1441 cagaccggcg gggtccagcc ggtcgtcgcc ggccgcgggc atggcgccga gcagggcgtc
     1501 cagccgggac gcgaattccg gctggccgct gtccagcacg ggggcagcca tcagccgcat
     1561 gtccgcgacg atctggccca tcaggcgacc ggcgccgggg atcaggtaaa tgccaccacg
     1621 ccggctcgcg ctggcggtga agcggtcaag cgccgcgtcg tcgatgccgc aggccaggcg
     1681 ttcgcgctgc atcgtagcgg ccggaagctg cggctggtcg gcatgcgccg cggccatctg
     1741 caacagggag aaggcgtatt ttacccggtc attggccgcc agcgcggcat tcacttcggc
     1801 gggccggcgc agatcctcgg ctccgatcgc acccagcacc ccggtcttca tgacgcgctc
     1861 cgattaccgg gcgggagtga agaccgggat ggcggcgggg cgccttgata tggctcaacc
     1921 cccaacccag cggcaggcgg tcagcgcccc gtcctgcatt ggatcgggcc gacatagtcg
     1981 cggtaggcag caacccaatc ggtggcgatc gcctgctgcg cggcgcgcag gctcagctcg
     2041 ccgtcgcaga cagcccgatg cagggcattt tccacccggt ccttggcatg cgccccgcag
     2101 tgtcccctcc acggctcggg ccacagattg cgcgggctgg acggcgcacc gccgagttcc
     2161 aggctgatca gatgatcctc ctcgtagtcc cgggtgcggc ggtcacgata cccccaggcg
     2221 cgcaactggt agcgtttcag gcggtaggtg tagtcctcgg ggggacggat atcgcgggtg
     2281 gaccaatgcc ggctgcagat cgtgcggtcg atggtggctt gcgtgattgc cgggtcgatc
     2341 gcccccggcg tacggtgcgg attgggcagg tcgcgcggcg gtgcggcggc gatcagcaac
     2401 agggcggcgg gcagcagcag gcattttgcc atggatcctc cgagtcttac ggctggccca
     2461 tgatccgcac tatccaccgg atgatccacc ggaattcagc acccagttga ttcaggtcaa
     2521 tgcagcggcc ggcgggccgc ccactaccgg tgcggcgatg ggcgtgacac atagcgggat
     2581 caacaagatt ttcgagggag cgcccgcatg ctgaccacca atgatttccg gctggatggg
     2641 cgggtcgccc tggtcaccgg ttcaggccgc aatatcggcc gcgccattgc cgaggcatat
     2701 gccgcggccg gcgcccgcgt cgtgctgaac ggacatcgcg acgccgccgc gctggaagag
     2761 gtcgcccagg gcatccgtgc ccgtggcggg gaagcgctgc ccatcctggc cgacgtcgcc
     2821 gacgatgccg ccgtgggccg catggtggac caggcggtgg ccacattcgg cagcgtggat
     2881 gtcgtcgtgt ccaatgtcgg catccgcaaa taccgcgcct tcctggagat cacccccgag
     2941 gagtgggatg cggtgctccg caccaacctg tccgcgtcgt tctacctggc ccgccacgcc
     3001 atcccgcaca tgcgcacgcg gaactgggga cgggtgatcg tgatctccgg tgccgacggg
     3061 ttctggggcc atgtcaccca ccgtgcgccg aacctcgccg caaaatccgg tatgcacggg
     3121 ctggccaagg cgatcgcacg cgaattcggc gccgacggca tcaccgcgaa cacggtgtcg
     3181 cccgggccga tcgacacggt gcgcgactgg tcgcaat
//
LOCUS       DASSZH010000644         6834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_441769, whole genome shotgun sequence.
ACCESSION   DASSZH010000644 DASSZH010000000
VERSION     DASSZH010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441769"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /gene="dnaN"
                     /locus_tag="VFN42_13940"
     CDS             <1..1041
                     /gene="dnaN"
                     /locus_tag="VFN42_13940"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019085734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HET8997765.1"
                     /translation="ILANVKIEARDVRLTLTATDMEIAVVEEVPATSLTDGACTAPAA
                     TLYEIVRKLPEGAEVQLDHPGGDAPLALRAGRYATSLVVLPTDDFPSMTAGSLPYRFA
                     LNAQHLRGLIDRTRFAISTEETRYYLNGIYLHATESEGVKVLRAVATDGHRLARVEEP
                     LPEGAAGMPGVIVPRKTVTELRKLLDEVSGDVDIALSDTRIQFQAGPVTLTSKLIDGT
                     FPEYERVIPRDNDKILRVGKKDFSDAVARVAAISAERSRPVKLSLGRNLLVLSASSPD
                     QGTATEELDGERVSYESTPLEIGFQARYLNDITDQVSGEVEFCFSDGAAPTIVRDAGD
                     ASALYVLMPMRV"
     gene            1059..2204
                     /gene="recF"
                     /locus_tag="VFN42_13945"
     CDS             1059..2204
                     /gene="recF"
                     /locus_tag="VFN42_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007400031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HET8997766.1"
                     /translation="MRLLRLTLTDFRTYATLSWRPAGHVAVLFGPNGSGKTNLLEAIS
                     LLVPGRGLRGARIADLARRGPGASGTWAVAARLATQAGETDIGTGTPPEGAADRRVFR
                     LDGAAPRSQAEVAARVAAVWLTPQMDRLFQEGPAGRRRFLDRLVFALEPGHAREVAAH
                     DTAMASRNRLLAAGGADTAWLAGLEDAMARHAVAVTAARATLAARLNASLAAGAAAPF
                     PAARLALLCPIAERLEGGPALAAEAWLRGALAANRARDAAAGSAAVGAHRADMALADA
                     ATGLPAEQASTGQQKALLIGVVLGHAALLAEARGFAPLLLLDEPTVHLDADRRAALFA
                     ALLRLPAQSFLTSADRDAFLPLRGTAEALRVTEGHVSHDAGFDAPSA"
     gene            2275..4707
                     /gene="gyrB"
                     /locus_tag="VFN42_13950"
     CDS             2275..4707
                     /gene="gyrB"
                     /locus_tag="VFN42_13950"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HET8997767.1"
                     /translation="MSDNAAPPPESDTYDASSISVLRGLDAVRKRPGMYIGDTDDGSG
                     LHHMAFEIIDNAVDEAQAGFATSVEVTLNGDGSVTVRDDGRGIPTDIHAEEGVSAAEV
                     VLTRLHAGGKFNQSSYKVSGGLHGVGAAVVNALSEWMDVRIWRNGQTHFIRFRNGDAE
                     APLAVTGPAERPNGTEVSFKPSPATFTRTEFEFSVLERRLRELAFLNSGLHIVLRDER
                     HPPAQECVFSYDGGLLAFVQWLDRAKVPVFAPPINIASAEGPHGIRVEFALSWNDSFH
                     ETMLCFTNNIPQRDGGTHLAGFRQALTRVVTKYAEGMGKKENLALVGEDMREGMTAVL
                     SVKVPDPKFSSQTKEKLVSSEVQPVVQAVAADAIAHWFETHPKEAKALVGKVMDAAAA
                     REAARKARDLTRRKGVLDVSTLPGKLADCQERDPALSELFIVEGDSAGGSAKQGRDRK
                     FQAILPLKGKILNVERARFDRMLSSAEIGTLITALGTGIGRAEPDQGGFDLSKLRYHR
                     IVIMTDADVDGSHIRTLLLTFFFRQMPLLIERGFLYIAQPPLYRAKRGSDERYLKDDA
                     ALERFLLDKALSEARLTYADGRVLEGGELRTEAAWLREATLRLRRLANGAPPAIVEQA
                     AIAGALTPDADRATAAAQMLVQRLDAISLPAERGWKVAADAAGLTLARTVRGVAERHV
                     LDATTLRSAEARWLAERTAALAERFAAPATLRLEAQEVSVAGPAAAFDRILAHGRRGL
                     TVQRFKGLGEMNPEQLWKTTLDPAVRTLLQVKVGDIEDAGQVFATLMGDVVEPRREFI
                     VGNALKVANLDV"
     gene            complement(4876..6279)
                     /locus_tag="VFN42_13955"
     CDS             complement(4876..6279)
                     /locus_tag="VFN42_13955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative O-glycosylation ligase, exosortase A
                     system-associated"
                     /protein_id="HET8997768.1"
                     /translation="MVRDLALVTAMLFYVPMSFRYPAAGALCWAWFSIMNPHRQVYGF
                     AFGQPFNSIIAIATLAGWLLSREPKRWTNDAVPWLLLILTGWMTLNTAVSADPSYSWP
                     FWDRVMRTLALIFLAFFLFTTKARIHAMVWTIVISLGFYGVKGGVFTIMNGGHAIVYG
                     PPDSVYADNNQLALAIVTDIPLLFYLWRYTKSPLLRWPMLAALALQVLMVFGSYSRGG
                     VIALGVMLTMLWLRSDRKILYGVLAALMVGAAINLMPDAFFHRMDTVNNLDGDDSFQG
                     RVNAWHVAFMYASEHFPFGAGFYALQREDIFHHYMPDAAMHAAHSIYFQMIGDQGFGG
                     FALYVLILLMALRNAGIVLRQTRGRPELRWAYDLADMSRVALIAFCFGGAALSMAYAD
                     EYLLIIALMSSLRQLTRPEALTAAAQTRHRSITSERDAVPALATTKKPGEVWQDGGKL
                     DLPAPGWATGRPDTSLR"
     gene            6390..6590
                     /locus_tag="VFN42_13960"
     CDS             6390..6590
                     /locus_tag="VFN42_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997769.1"
                     /translation="MSIVGIGLVMRWMAINDRVAPDQPTTGLFAMLETARRRLREERT
                     VPEPDAHRPAAKRPDARRQRQT"
BASE COUNT         1206 a         2290 c         2280 g         1058 t
ORIGIN      
        1 atcctggcca acgtgaagat cgaggcgcgc gacgtgcggc tgacgctgac cgcgaccgat
       61 atggagatcg cggtggtgga ggaggtgccg gccaccagcc tgaccgacgg cgcctgcacc
      121 gccccggcgg cgacgctgta cgagatcgtc cgcaagctgc ccgaaggggc ggaagtgcag
      181 ttggaccacc ccggcggcga cgcgccgctg gcgctgcggg cggggcgcta cgccaccagc
      241 ctggtggtgc tgccgaccga cgacttcccc tccatgaccg ccggcagcct gccctaccgc
      301 ttcgcgctca acgcgcagca tttgcgcggc ctgatcgacc gcacccgctt cgccatctcc
      361 accgaggaga cgcggtacta tctgaacggc atctatctcc acgcgaccga gagcgagggg
      421 gtgaaggtgc tgcgcgcggt cgccaccgac ggccaccgcc tcgcccgcgt cgaggagccg
      481 ctgccggagg gggcggccgg gatgccgggc gtgatcgtgc cgcgcaagac ggtgacggaa
      541 ctgcgcaagc tgctcgatga ggtcagcggc gacgtggaca tcgcgctgtc cgacacgcgc
      601 atccagttcc aggccgggcc ggtgacgctg accagcaagc tgatcgacgg caccttcccg
      661 gaatacgagc gcgtcatccc gcgcgacaac gacaagatcc tgcgcgtcgg caagaaggat
      721 ttctccgatg ccgtggcccg cgtcgccgcc atctcggcgg agcggtcgcg gccggtgaaa
      781 ctgtcgctgg ggcgcaacct gctggtgctg tccgcctcca gccccgacca gggcacggcg
      841 acggaggaac tggacggcga gcgggtgtcg tatgagtcga cgccgctgga gatcggcttc
      901 caggcgcgct acctcaacga catcaccgac caggtgtccg gcgaggtgga gttctgcttc
      961 tccgacggcg cggcgccgac catcgtgcgg gatgcggggg acgccagcgc gctttatgtg
     1021 ctgatgccga tgcgcgtctg aatgtgccca cgccgggatt gcgcctgctc cggctgacgc
     1081 tgaccgattt ccgcacctac gcgacactct cctggcggcc ggccggccac gtcgccgttc
     1141 tgttcgggcc gaacggcagc ggcaagacca atctgctgga ggcgatctcc ctgctcgtgc
     1201 cgggccgcgg cctgcgcggc gcgcgcatcg ccgacctggc gcggcgcggg ccgggcgcgt
     1261 ccgggacctg ggcggtcgcg gcgcggctgg cgacccaggc gggcgagacc gatatcggca
     1321 ccggcacgcc gccggagggt gccgcggatc ggcgcgtgtt ccgcctcgac ggtgccgccc
     1381 cgcgcagcca ggcggaggtg gcggcgcgcg tcgccgcggt gtggctgaca ccgcagatgg
     1441 accggctgtt ccaggaaggc cccgccggcc gccgccgctt cctcgaccgg ctggtgttcg
     1501 cgctggaacc cgggcacgcg cgggaggtgg cggcccacga cacggccatg gccagccgca
     1561 accgcctgct cgccgccggc ggcgccgata cggcgtggct ggcggggctg gaggatgcga
     1621 tggcccgcca tgccgtcgcg gtcaccgccg cccgcgccac gctggccgcg cggctgaacg
     1681 cgtcgcttgc cgccggtgcg gccgccccct tccccgccgc gcgccttgcc ctgctctgcc
     1741 cgatcgccga acggctggag ggcgggccgg cactggcggc ggaggcgtgg ctgcgcggtg
     1801 ccctcgccgc caaccgcgcg cgcgacgccg ccgccgggtc cgccgcggtc ggggcgcacc
     1861 gggccgacat ggcgctggcc gacgccgcga ccgggctgcc ggccgaacag gccagcaccg
     1921 ggcagcagaa ggcgctgctg atcggcgtgg tgcttggcca tgccgcgctg ctagccgagg
     1981 cgcgcggctt cgccccgctg ctgctgctcg acgaacctac cgtgcatctc gacgcggatc
     2041 gccgcgcggc gctgttcgca gcactgctgc ggctgccggc gcaatcgttc ctgacatcgg
     2101 ctgaccgcga cgcgttcctg cccctgcgcg gtacggcgga ggcgctgcgc gtgacggaag
     2161 gtcatgtgtc tcacgatgcc ggattcgacg cgccatcggc gtgacccctg tggcaaaccc
     2221 cgcgcaattc ctatatgttt gtcattcccg cacgccctgg agcacatcgc cggcatgagc
     2281 gacaacgccg cgccaccgcc cgaatccgac acctacgacg cctcctccat ttccgtcctg
     2341 cgtggccttg atgccgtgcg caagcggccg ggcatgtata tcggcgacac cgatgacggc
     2401 tccggcctgc atcacatggc tttcgagatc atcgacaacg cggtcgatga ggcgcaggcc
     2461 ggtttcgcga ccagtgtcga ggtgacgctg aacggcgacg gcagcgtcac cgtgcgcgac
     2521 gacggccgcg gcatccccac cgacatccac gcggaggaag gcgtgtccgc ggccgaggtg
     2581 gtgctgacgc ggctgcatgc cggcggcaag ttcaaccaga gttcctacaa ggtttccggc
     2641 ggcctgcacg gcgtcggcgc cgcggtggtg aatgcgctgt ccgaatggat ggacgtgcgc
     2701 atctggcgta acggacagac gcatttcatc cgcttccgca acggcgacgc cgaggcaccg
     2761 ctcgccgtca ccggccctgc cgagcgaccg aacggcaccg aggtatcgtt caagccttcg
     2821 cccgccacct tcacccgcac cgaattcgaa ttctccgtgc tggagcggcg gttgcgcgaa
     2881 ctggcgttcc tcaactccgg cctgcacatc gtgctgcgcg acgagcggca tccgccggcg
     2941 caggaatgcg tcttctccta cgacggcggc ctgcttgcct tcgtgcagtg gctggaccgc
     3001 gccaaggttc ccgtgttcgc gccgccgatc aacatcgcct ccgccgaagg gccgcacggc
     3061 atccgcgtgg aattcgcgct gtcgtggaac gacagtttcc acgagaccat gctctgcttc
     3121 accaacaaca tcccgcagcg cgacgggggc acgcatctcg ccgggttccg ccaggcgctg
     3181 acgcgcgtgg tgaccaaata tgccgaaggg atgggcaaga aggaaaatct tgccctggtc
     3241 ggcgaagata tgcgcgaggg catgacggcg gtactgtcgg tgaaggtgcc cgatccgaaa
     3301 ttttcctcgc agacgaagga aaaactcgtc agcagcgagg tgcagccggt ggtgcaggcg
     3361 gtcgcggcgg atgcgatcgc ccactggttc gagacgcatc ccaaggaagc caaggcgctg
     3421 gtcggcaagg tgatggacgc cgcggcggcg cgggaggcgg cgcgcaaggc gcgcgacctg
     3481 acgcggcgca agggcgtgct ggatgtctcc acgctgcccg gcaagctcgc cgactgccag
     3541 gaacgcgatc cggcgctgtc cgagttgttc atcgtcgagg gcgattccgc aggcggctcc
     3601 gccaagcagg gccgcgaccg gaagtttcag gcgatcctgc cgctgaaagg caagatcctg
     3661 aacgtcgagc gcgcgcgctt cgaccgcatg ctgagcagcg cggagatcgg cacgctgatc
     3721 accgcgctcg gcaccggcat cggccgcgcg gaacccgacc agggcgggtt cgacctgtcg
     3781 aagctgcgct accaccgcat cgtcatcatg accgacgcgg acgtggacgg atcgcatatc
     3841 cgcacgctgc tgctgacgtt cttcttccgc cagatgccgc tactgatcga gcgcggcttc
     3901 ctctacatcg cgcagccgcc gctctatcgc gccaagcgcg gcagcgacga acgctacctg
     3961 aaggacgacg ccgcgctgga gcggttcctg ctcgacaagg cgctgtccga ggcacgcctg
     4021 acctatgccg acggccgcgt gctggagggc ggggaactgc gcaccgaagc ggcgtggctg
     4081 cgtgaggcga cgctgcggct gcgacggctg gcaaacggcg caccgccggc gatcgtcgag
     4141 caggcggcga tcgccggcgc gctgacaccg gatgccgacc gtgcgacggc agcggcgcag
     4201 atgctggtgc aacggctcga tgcgatctca cttcccgcag agcgtggctg gaaggttgcc
     4261 gccgatgccg ccgggctgac acttgcgcgc acggtgcgcg gcgtcgccga gcggcatgtg
     4321 ctggacgcga ccacgctgcg cagcgcggag gcccgctggc ttgccgaacg caccgccgcg
     4381 ctcgccgaac gctttgccgc gccggcaaca ctacggctgg aggcacagga agtttcggtc
     4441 gccggccctg cggccgcctt cgaccgcatc ctcgcgcatg gtcgccgcgg cctgacggtg
     4501 cagcgcttca aaggtctcgg tgaaatgaac cccgagcagc tttggaagac cacgctcgat
     4561 cccgcggtgc gcacgttgct gcaggtaaag gtgggcgaca tcgaggatgc cgggcaggtg
     4621 ttcgcaactc tgatgggcga cgtggtcgag ccgcggcgcg agttcatcgt cggcaatgcg
     4681 ctgaaggtag cgaacctgga cgtgtgacca cctcggacgc agccacaggt cccggaacag
     4741 cacgtcggtg gtgaaccggt tgaggcccgg agcgtccggg ccgaccggtt tccccgacgc
     4801 gggccgcgcg ctgcgcgcca gccgccagaa tcgaaccagg ccgagacgaa tatgggcctt
     4861 gcaggcgaat gcgacttagc gaaggctggt atcaggccgg cctgtcgccc agccaggggc
     4921 cggaaggtcg agctttccgc catcctgcca cacttcaccc ggcttcttcg tcgtcgccag
     4981 ggccggtacg gcatcccgct ccgacgtgat gctacggtgg cgtgtctgcg ccgcggcggt
     5041 gagggcttcc ggtcgggtca gttggcgtag cgacgacatc agcgcgatga tcaacagata
     5101 ctcatcggcg taggccatgc tcagcgccgc gccgccgaaa cagaatgcga tcagtgccac
     5161 ccgactcatg tccgccaagt cgtacgccca gcgcaattcg ggccggccac gcgtttgtcg
     5221 cagcacgata cccgcattgc gcaaagccat caacagtata agcacataca gcgcgaaacc
     5281 accaaacccc tggtctccaa tcatctggaa ataaatgcta tgagcggcat gcatcgccgc
     5341 gtctggcata tagtggtgaa aaatatcctc tctctgcagg gcataaaagc cggcgccaaa
     5401 cgggaaatgc tcgctggcat acatgaaggc cacatgccag gcgttcacgc gtccctggaa
     5461 cgaatcgtca ccgtcaagat tgttgactgt atccatccgg tgaaaaaagg catccggcat
     5521 aagattgatt gcggcgccga ccatcaacgc agccagaacg ccgtacagta tcttgcggtc
     5581 gctgcgcagc cacagcatcg tcaacatgac gccgagagca atcaccccgc cgcgggaata
     5641 cgacccaaac accataagca cctgcaaggc caaggccgcc agcatgggcc agcgcaacaa
     5701 cggcgacttg gtgtaacgcc ataaataaaa cagcaacggt atatcagtaa cgattgccag
     5761 agccaactgg ttattatccg cataaacgct gtccggcggg ccataaacga tggcgtggcc
     5821 accgttcatg attgtaaaca ccccgccctt cacgccgtag aagccgagtg aaatgacaat
     5881 cgtccatacc atggcgtgaa ttcgcgcctt ggtggtgaac aggaagaacg ccaaaaagat
     5941 cagggccagg gtacgcatca cccggtccca gaaaggccac gaatagctcg ggtccgcact
     6001 gacggccgtg ttcaacgtca tccatcccgt cagaatcagc agcagccagg gcaccgcatc
     6061 gttggtccag cgcttgggct cgcgcgacag cagccagccc gccagggttg cgatcgcaat
     6121 gatactgttg aacggttgtc cgaaggcgaa gccatagacc tgccggtgcg gattcatgat
     6181 cgaaaaccat gcccagcaca gcgcccccgc agccgggtag cggaacgaca tcggcacgta
     6241 gaacagcatg gccgtcacaa gggcaagatc gcgcaccacg ggatgaacct gctatggcaa
     6301 tgttggtccg ataggcgttc atacaccgat tcggactccg tccgtggctg catctggagc
     6361 ggaagtcaat ggaaggtctt ttcttgttga tgtcgatcgt cggcatcggg ctggtgatgc
     6421 gatggatggc gatcaacgac cgggtcgcgc cggaccagcc gacgaccggc ctgttcgcca
     6481 tgctcgaaac ggccaggcga cgtctgcgcg aggaacggac cgtgcccgag ccggatgcgc
     6541 accgtccggc agcaaaacgg ccggacgccc gcaggcagcg gcagacgtga gcgcgtcgcg
     6601 ggaggcacga ttcgccctgg agcacgggga tcacgcaatg cgcctccacg tctcagcatt
     6661 cgttatcatt atgccgttcg gacctgccgc tgacctggat atgctcaagg ggagcctcat
     6721 atctccctat tcatgattgg attgtaccat ttaatattgc tgtataagaa tgaaaccaag
     6781 tgcattttgc aggaattgtg ataagtgtgt ggatgcgtta tagttcgtta tgtc
//
LOCUS       DASSZH010000645         3129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_578073, whole genome shotgun sequence.
ACCESSION   DASSZH010000645 DASSZH010000000
VERSION     DASSZH010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578073"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /locus_tag="VFN42_13965"
     CDS             <1..1256
                     /locus_tag="VFN42_13965"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET8997770.1"
                     /translation="IAAEYPPGTEETRTMPESPLDKSMLTETRMPLNGITVLDLTLAR
                     AGPTAVRHFADWGANVIRIEPPPTDGEDVAGRRHGFDFQNLHRNKRAITLNLKTPEGH
                     AAFMRLAEKADVILENMRAQVKHRLKVSYEDVRAINPRIVYGSISGFGQDGPYGKRAG
                     VDQIAQGMGGLMSITGEPGRGPMRVGIPIDDLTAGNLLAMGCLMALFDRERTGVGRWV
                     TTSLLEAQIFMLDFQASRWLMEGEVAGQAGNDHPTGIPTGVFPTADGHINIAASSSRV
                     FARFCEAIGRPDWLEKEEWKTQQGRSKDRKAINAAISEITRTRPSEHWIELFEAAGCP
                     CGPINTIDKVFADPQVKHLGMARAMHSPVLGDTQVVSSAINISGFSKDIRSPTPEAGQ
                     HTDEVLSAVGYSADEIADMRKKGAI"
     gene            1257..2075
                     /locus_tag="VFN42_13970"
     CDS             1257..2075
                     /locus_tag="VFN42_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET8997771.1"
                     /translation="MLPEHAETKAYAGGKMLAAKDAGVGLVTFNQPEKRNAMSIEMWQ
                     GLSEILDEFREDSSVRVVVMTGAGNKAFVSGADISQFEKNRSNADAQQEYDRLTTGGR
                     TKLTQFPKPVIARIRGFCLGGGLALAMSTDIRIAGADSQFGIPAARLSIAYAPESVRK
                     LIDLVGPAHARMILYSARRIDGAEAERIGLINKMVPDEALDDHVMDLARTIADNAPLS
                     IAASKLTINELLKDESQRNTGAMREQMDICFNSADYKEGRTAFMEKRAPRWQGR"
     gene            2093..2914
                     /locus_tag="VFN42_13975"
     CDS             2093..2914
                     /locus_tag="VFN42_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET8997772.1"
                     /translation="MPNASGGRIRVGIGGWTFPPWRGAFYPQALPHTRELDYASRHVT
                     SIEINGTYYRLQRPESFARWREETPDDFVFALKAPRYVTNRRVLAEAGKSMQRFLDSG
                     LVELQHKLGPINWQFLPTKQYDAADFEAFLKLLPAEVQGHPLRHVVEVRHPSFAHPDF
                     IALLRAHGIGLVVADSADYPALHDPTAPFIYARLQSASEQEAAGYPDTALERWAERAH
                     AWATGRTPEDLPLLAPTPRKASQGRDVFIYMINGFKPKAPAAAMALLDRLSRKRG"
BASE COUNT          586 a         1022 c         1049 g          472 t
ORIGIN      
        1 cgattgcggc cgaatatccc cccggaacgg aggagaccag aacgatgccg gaaagcccgt
       61 tggacaagtc gatgctgacc gagacccgca tgccgctgaa cggcatcacc gtgctcgacc
      121 tgacgctggc gcgtgccggc ccgaccgcgg tgcgccattt cgccgattgg ggcgccaacg
      181 tgatccgcat cgagccgccg ccgaccgatg gcgaggatgt cgccggccgc cgccacggct
      241 tcgacttcca gaacctgcac cgcaacaagc gcgccatcac gctgaacctg aagacgccgg
      301 aaggccacgc cgccttcatg cgcctggcgg aaaaggccga tgtgatcctg gagaatatgc
      361 gggcccaggt gaagcaccgg ctgaaagtct catacgagga cgtgcgggcg atcaacccgc
      421 gcatcgtcta tggcagcatc tccggtttcg gccaggacgg gccgtacggc aagcgcgcgg
      481 gcgtggacca gatcgctcag ggcatgggcg ggctgatgtc gatcaccggc gagccgggcc
      541 gcggaccgat gcgcgtcggc attccgatcg acgacctgac cgccggcaac ctgctggcga
      601 tgggctgcct gatggcgctg ttcgaccgcg aacgcaccgg cgtcggccgc tgggtcacca
      661 cctccctgct ggaggcgcag atcttcatgc tcgacttcca ggccagccgc tggctgatgg
      721 aaggcgaggt ggccggccag gccggcaacg accacccgac cggcattccc accggcgtgt
      781 tccccaccgc cgacgggcac atcaacatcg ccgccagttc ctcccgtgtg ttcgcgcggt
      841 tctgcgaagc gatcggccgg ccggactggc tggagaagga ggagtggaag acgcaacaag
      901 gccgcagcaa ggaccgcaag gcgatcaacg ccgcaatcag cgagatcacc cggaccaggc
      961 cgtccgagca ctggatcgag ctgttcgagg cggccggctg cccgtgcggg ccgatcaaca
     1021 cgatcgacaa ggtgttcgcc gatccgcagg tgaagcacct ggggatggcg cgcgcgatgc
     1081 atagtccggt gctgggcgac acgcaggtgg tgtcgtcggc gatcaacatc tccggattct
     1141 ccaaggacat ccggtcgccg acgccggagg ccggccagca cacggatgaa gtcctgtcgg
     1201 cggtgggcta tagcgcggac gagatcgccg acatgcgcaa gaaaggggct atctgaatgc
     1261 tgccggaaca cgcggaaacc aaggcttatg ccgggggcaa gatgctcgcg gcgaaggacg
     1321 ccggggtggg cctggtcacc ttcaaccagc ccgagaagcg caacgccatg tcgatcgaga
     1381 tgtggcaggg tctgagcgaa atcctggacg agttccggga ggacagctcg gtccgcgtcg
     1441 tggtgatgac cggtgccggc aacaaggcat tcgtgtccgg ggccgacatt agccagttcg
     1501 agaagaaccg ctcgaatgcc gacgcgcagc aggagtacga ccgcctgacc accggcgggc
     1561 ggaccaagtt gacgcagttt cccaagccgg tgatcgcccg tatccgcggc ttctgcctgg
     1621 gcggcggcct ggcgctggcg atgagtaccg acatccgcat cgccggcgcc gacagccagt
     1681 tcggtatccc ggccgcgcgg ctgtcgatcg cctatgcgcc ggagtccgtg cgcaagctga
     1741 tcgatctggt cggaccggcg catgcgcgca tgattctcta cagcgcgcgg cggatcgatg
     1801 gggcggaggc ggagcggatc gggctgatca acaagatggt accggatgag gcactggacg
     1861 accatgtgat ggatctggcg cgcaccattg ccgacaacgc gccgctgtcg atcgccgcat
     1921 ccaagctgac catcaacgaa ctgctgaagg acgagagtca gcgcaacacc ggcgcgatgc
     1981 gcgagcagat ggacatctgc ttcaacagcg ccgactacaa ggaaggccgc accgcgttca
     2041 tggagaagcg ggcgccgcgc tggcaggggc gatagcggca agcaggtccg gcatgccgaa
     2101 cgccagtggc ggaagaatcc gggttggcat cggcggctgg accttcccgc cctggcgtgg
     2161 cgcgttctac ccgcaagcgc tgccgcatac gcgcgagctg gattatgcca gccgccacgt
     2221 cacctcgatc gagatcaacg gcacgtacta tcgcctgcag aggccggaga gtttcgcgcg
     2281 ctggcgcgaa gagacgccgg acgacttcgt gttcgcgctg aaggcgccgc gctacgtcac
     2341 caaccgccgc gtgctggccg aagccggcaa atcgatgcag cgtttcctcg acagcgggtt
     2401 agtggaactg cagcacaagc tcggtccgat caactggcag ttcctgccga ccaagcaata
     2461 cgacgcggcc gatttcgagg cgttcctgaa gctgctgccc gctgaggtgc aaggccatcc
     2521 cttgcgccac gtggtggaag tgcgccaccc gagctttgcg catcccgatt tcatcgcgct
     2581 gctgcgcgcg cacggcatcg gtctggtggt cgccgatagt gcggattatc cggcgctgca
     2641 cgatccgacg gcgcccttca tctacgcccg cctgcagtcg gcctccgaac aggaagccgc
     2701 cggctatccg gacacggcgc tggagcgctg ggcggagcgg gcgcatgcct gggcgacagg
     2761 ccgtacgcca gaggacctgc cgttgctcgc gcccaccccg cgcaaggcat cccagggccg
     2821 cgacgtgttc atctacatga tcaacggctt caagccgaag gcgccggccg ccgccatggc
     2881 actgctcgat cgcctgtcgc gaaagcgggg ctgacgctgg gccgggcttc tggaacgaat
     2941 gaagattctg caatgtgatt gatctagatc agggaccgct tcctgtcggg cagccacgtt
     3001 gcgtttatcg aaggaatcgc cccagaagga accccgcgat ggcctacctc gcaccgattg
     3061 ttcccgccga agcaaccgct ttcagcccgc tgatgctgtc cgaccgcctg ctgaccctgg
     3121 cgcaggacg
//
LOCUS       DASSZH010000646         4765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1106927, whole genome shotgun sequence.
ACCESSION   DASSZH010000646 DASSZH010000000
VERSION     DASSZH010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106927"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..768)
                     /gene="fliP"
                     /locus_tag="VFN42_13980"
     CDS             complement(43..768)
                     /gene="fliP"
                     /locus_tag="VFN42_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018980924.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HET8997773.1"
                     /translation="MIALAVLLLPAAAAAQSVSIDLGSGGQAGVTSRIVQLTALIAVL
                     SLAPSLLVMVTAFTRIIIVLSLLRSALSTQGAPPNMVLIGLALFLTFFVMQPVLDQSW
                     TAGLAPMAAGKMPEIEGMRLAAAPFHRFMTDNLRPADLQFFLNLGHMPPSASADLAPW
                     RAVVPAFIVGELRRAFEMGFLLFLPFLVIDMVVASVLMSLGMMMLPPSVVSLPFKLIF
                     FVLVDGWRLVSGSLVQSFGVSPS"
     gene            complement(789..1091)
                     /locus_tag="VFN42_13985"
     CDS             complement(789..1091)
                     /locus_tag="VFN42_13985"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HET8997774.1"
                     /translation="MLPNLSALLSALAALAVVLVLIWVAARGARLAGFRPRPAAGRML
                     KPLDSIALDSRRRLHLVACQQRQVLLLTGGATDLVVGWLPDQPAQPSSQLAAEQQP"
     gene            complement(1166..1912)
                     /locus_tag="VFN42_13990"
     CDS             complement(1166..1912)
                     /locus_tag="VFN42_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8997775.1"
                     /translation="MKLDGKTALVTGGVSGIGKATALEFARAGARVVVADVNRDGGSA
                     IETEAKAAGLPIEYVHLDLADLASIDACAAAVLARYQRVDIQVNAAGWDRIQPFLENT
                     PEFMDRVLAINLRGPIGLTQKLVPAMAAAGYGKIVNVASDAGRVGSLGETVYAAAKGG
                     IIAFTKSLAREVARYNITVNCVCPGPTDTPLFHAQPDKLKVALEKAIPFKRIAKAQEL
                     ADAILFFASPRSDYVTAQVLSVSGGLTMVG"
     gene            complement(1925..3058)
                     /locus_tag="VFN42_13995"
     CDS             complement(1925..3058)
                     /locus_tag="VFN42_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET8997776.1"
                     /translation="MILTEEQEMIRDTAREFARRHLAPWSATWERDATFPREALREMG
                     KLGLLGMTVSPEWGGAGTDYVSYALALEEIAAGDAATATVMSGHNSVGCMPIAVFGTE
                     EQKQRYLKPMAAGTYLSAFALTEPSGGSDAGALRSRAERRGDVYVLNGSKQYITTGSN
                     AEICLVFASTDPSQGKRGISAFIVETHLPGYHVGRIEKKMGQNASDTCEIQFDNLEIP
                     VANRLGAEGQGYRIALSNLEGGRIGIAAQAVGIARAAYELALAYAQERRSFGKPIYEY
                     QAVSHRIADMATNILAARQLVLHAAALRSAGRPCLTEASMAKLFASEMAERVCSDAVQ
                     TFGGAGYVADSGVEQLYRAVRVTRIYEGTNDIQRLVISRALAN"
     gene            3179..3973
                     /locus_tag="VFN42_14000"
     CDS             3179..3973
                     /locus_tag="VFN42_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016079909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET8997777.1"
                     /translation="MAEASRYTDLLYEEKDGVATITMNRPEKYNAFRGITCDELIDAF
                     NRAGWNKRIGCIVLTGAGSKAFCTGGDQSAHEGAYDGKGTVGLPMEALHNIIRGVPQP
                     VIAKVRGYAIGGGNVLATLCDLTIASETAQFGQVGPKMGSVDPGFGTAYLSRIVGEKK
                     AREIWYLCRRYTAQEAQAMGLVNKVVPDDQLDAEVESWAKEIVERSPTAIAIAKRSFN
                     ADSDSIQGIGALGMQALKLFYESAESQEGVASFLEKRKPDFRKYYR"
     gene            4169..>4765
                     /locus_tag="VFN42_14005"
     CDS             4169..>4765
                     /locus_tag="VFN42_14005"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET8997778.1"
                     /translation="MSIDPILPPERIRQMKQAGFWGERILLDDLDHWTATVPDRIAII
                     DHNSMTGERTELSYAAFADRVDRIAAGLHRLGIRKGDVVSLQLPNWWMFNALHFAALR
                     IGAVTNPIMPIFRERELGFMLQMAESKLLVIPREFRGFAYQPMAAALRPALPALQHVL
                     VVGGEGDAAFDRLYRDTPPPDPAMRAHFAAARPSADDVV"
BASE COUNT          818 a         1565 c         1629 g          753 t
ORIGIN      
        1 agccccggga gcgaattgcc gacggttgtt cagccgctgc agttacgatg gcgacacacc
       61 gaaactctgc accagactgc ctgacaccag gcgccagcca tcgacgagga caaagaagat
      121 cagcttgaac ggcagcgaca cgacgctggg cggcagcatc atcatgccca ggctcatcag
      181 cacgctggcg accaccatgt cgatcaccag gaagggcagg aacagcagga accccatctc
      241 gaaggcgcgg cgcagctcgc ccacgatgaa ggcggggacc accgcgcgcc acggcgccag
      301 gtccgcgctg gcggatggcg gcatatggcc gaggttgagg aagaactgca gatcggccgg
      361 ccgcagattg tcggtcatga agcggtggaa cggggcggcg gccagccgca tgccctcgat
      421 ctcgggcatc ttgcccgcgg ccatcggcgc cagcccggcc gtccaggact gatccagcac
      481 cggctgcatg acgaagaagg tgaggaacag cgccagcccg atcagcacca tgttgggcgg
      541 cgcgccctgg gtgctgagcg cgctgcgcag cagcgacagc acgatgatga tgcgggtgaa
      601 ggcggtgacc atcaccagca ggctgggcgc cagcgacagc accgcgatca gcgcagtcag
      661 ctgcacgatg cggctggtga cgccggcctg tccaccgctg cccaggtcga tgctgaccga
      721 ttgggccgcc gccgcggcgg gcagcagcag caccgccagc gcgatgatgc aggcgcagag
      781 gcggcggatc acggctgctg ctccgcggcg agctggctgg agggctgcgc cggttggtcg
      841 ggaagccagc ccaccaccag gtcggtcgca ccgccggtca gcagcagtac ctggcgctgt
      901 tggcaggcca ccagatgcag gcggcggcgg gaatccagcg caatgctgtc gagcggcttc
      961 agcatccgcc cggcggccgg gcgcggccta aacccggcga ggcgggcgcc gcgcgccgcc
     1021 acccagatca acaccagtac gaccgccagg gcggcgagcg cggaaagcag ggcagagagg
     1081 ttgggcagca tgtggcgcgg ccttttgccg gcagtcggga ccgcgccatt gcagcagaaa
     1141 gggatgaaga aacggtttgc gccggctacc ccaccatcgt cagcccgccg ctgacgctga
     1201 gcacctgcgc ggtgacgtag tcggaccgcg ggctggcgaa gaacaggatc gcatccgcca
     1261 gctcctgcgc cttggcgatg cgcttgaacg gaatcgcctt ctccagcgcg accttcagct
     1321 tgtccggctg cgcatggaac agtggcgtgt cggtcgggcc cgggcacacg cagttcaccg
     1381 tgatgttgta gcgcgccacc tcgcgtgcca gcgacttggt gaaggcgatg atgccgccct
     1441 tggccgccgc gtacacggtt tcccccaggc tgccgacgcg gcccgcatcg ctggcgacgt
     1501 tgacgatctt gccgtaaccc gctgccgcca tcgccggcac cagtttctgc gtcagtccga
     1561 tcggcccgcg caggttgatc gccagcacgc ggtccatgaa ctccggcgtg ttctccagga
     1621 agggctggat gcggtcccag ccggcggcgt tcacctggat gtccacgcgc tgataccgcg
     1681 ccaacaccgc cgcggcgcag gcatcgatcg aggccagatc ggcaaggtcg agatgcacgt
     1741 attcgatcgg cagcccggcc gccttcgcct cggtttcgat tgccgaaccg ccgtcgcggt
     1801 tgacatccgc caccaccacg cgcgcgccgg cacgggcgaa ttccagtgcg gtggccttgc
     1861 cgatgcctga tacgccgccg gtgaccaggg ccgttttgcc gtccagcttc atggtgcttc
     1921 cttctcagtt ggccagcgcc cggctgatca ccaggcgctg gatatcattg gtgccttcat
     1981 agatgcgggt gacgcgcacg gcgcggtaca gctgctccac gccggaatcg gcgacatagc
     2041 cggcgccgcc gaaggtctgc accgcgtcgg agcacacgcg ttccgccatt tccgacgcga
     2101 acagcttggc catcgacgcc tccgtgaggc aaggccggcc ggcgctgcgc agcgcggcgg
     2161 catgcagcac cagttgccgc gccgccagga tgttggtcgc catgtcggcg atgcggtgcg
     2221 acacggcttg atattcatag atcggcttgc cgaagctgcg ccgctcctgc gcataggcca
     2281 gcgccagttc ataggcggcg cgggcaatcc ccacggcctg cgcggcgata ccgatgcggc
     2341 cgccttccag gttggacagc gcgatccgat agccctggcc ctcggcgccc agccggttgg
     2401 cgacgggtat ctccaggttg tcgaactgga tttcgcaggt gtcggaggcg ttctgcccca
     2461 tcttcttttc gatgcggccg acatggtagc cgggcaggtg agtctccacg atgaacgccg
     2521 agatgccgcg cttgccttgc gagggatcgg tgctggcgaa caccaggcag atctcggcat
     2581 tgctgccggt ggtgatgtac tgcttgctgc cgttcagcac gtacacgtcg ccgcggcgtt
     2641 ccgcgcggct gcgcagcgcg ccggcatccg aaccgccgct cggttcggtc agtgcgaagg
     2701 cgctgagata ggtaccggcg gccatcggtt tcaggtatcg ctgcttctgt tcctcggtgc
     2761 cgaacaccgc gatgggcatg cagccgacgg agttgtggcc gctcatcacc gtggcggtgg
     2821 cggcgtcgcc ggcggcgatt tcctccagtg ccagcgcgta ggaaacgtag tcggttccgg
     2881 cgccgcccca ttccggcgat acggtcatgc cgagcaggcc cagcttgccc atttcgcgca
     2941 gggcttcgcg cgggaaggtg gcgtcgcgtt cccaggtggc ggaccacggt gccaggtgcc
     3001 tgcgcgcgaa ttcccgcgcg gtgtcccgga tcatctcttg ttcttcagtg aggatcatgg
     3061 cacaagcggt ctggagtgaa cgagcgttca gtcggttggc actgtgaccc tgccgcggtc
     3121 ccaagtcaag cggcgcaacc ggccggcgcc ttgctgttct gcaacaggag aaacctgcat
     3181 ggccgaagcg tcccgctaca ccgacctgct gtatgaggag aaggacggtg tcgccaccat
     3241 cacgatgaac cgtcccgaga aatacaacgc gtttcgtggc atcacctgcg acgagctgat
     3301 cgacgcattc aaccgggccg gctggaacaa gcggatcggc tgcatcgtgc tgaccggcgc
     3361 gggtagcaag gcgttctgca cgggcggcga tcaatcggcg catgaggggg cgtatgacgg
     3421 caagggcacg gtgggcctgc cgatggaggc gctgcacaat atcatccgtg gtgtgccgca
     3481 gccggtgatc gccaaggtgc gcggctatgc gatcggcggc ggcaacgtgc tggcgacgct
     3541 gtgcgacctg acgatcgcgt cggagacggc gcagttcggc caggtcgggc cgaagatggg
     3601 ctcggtcgat ccgggcttcg gcaccgccta cctgtcgcgc atcgtggggg aaaagaaggc
     3661 gcgggagatc tggtacctgt gccggcgcta caccgcgcag gaagcgcagg cgatggggct
     3721 ggtgaacaag gtggtgccgg acgaccagct ggatgcggaa gtcgagagct gggcaaagga
     3781 gatcgtcgag cgcagcccga cggcgatcgc catcgccaag cggtcgttca acgcggattc
     3841 cgacagcatc caggggatcg gcgcgctggg catgcaggcg ctgaagctgt tctacgaaag
     3901 cgcggaatcg caggagggcg tggcgtcctt cctggagaag cgcaagccgg atttccgcaa
     3961 atactatcgg tagccgcatg cgtctcggcc ggcgaaggca ccccttttgt catggccggc
     4021 gaaggccggc cacccacgtc ttttcttcct gcacgcaaca agacgtgggt ggccagggta
     4081 agcccggcca tgacgcgggg ggcgacagaa taaacgaacg ctcgttcagt tatcagggga
     4141 acgagccaaa agccccggag gaatcgtcat gagcatcgac ccgatcctgc cgcccgagcg
     4201 catccggcag atgaaacagg ccggcttctg gggcgaacgc atcctgctgg atgacctgga
     4261 tcactggacc gccaccgtcc cggaccgcat cgccatcatc gaccacaaca gcatgactgg
     4321 cgagcgcacc gaactctcct acgccgcgtt cgccgaccgg gtggatcgca ttgctgccgg
     4381 gctgcaccgg ctgggcatcc gcaagggcga cgtggtttcg ctgcaactcc ccaactggtg
     4441 gatgttcaac gcgctgcact tcgccgcatt gcgcatcggc gccgtcacca acccgatcat
     4501 gccgattttc cgcgagcgcg agctcggctt catgctgcaa atggcggaat cgaagctgct
     4561 ggtgatcccg cgcgaattcc gcggcttcgc ctaccagccc atggccgcgg cactgcggcc
     4621 cgcgctgccg gcgctgcagc acgtgctggt ggtcggcggc gagggcgatg ccgcgttcga
     4681 ccgcctgtac cgcgacacgc caccccccga tcccgccatg cgcgcgcatt tcgccgcggc
     4741 tcggccgagc gcggatgatg tggtg
//
LOCUS       DASSZH010000647         3756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_459102, whole genome shotgun sequence.
ACCESSION   DASSZH010000647 DASSZH010000000
VERSION     DASSZH010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459102"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..779
                     /locus_tag="VFN42_14010"
     CDS             213..779
                     /locus_tag="VFN42_14010"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8997779.1"
                     /translation="MDADAVRAMGRPRGLPFKLNKLGHIALYVQDLQRSARFYTEVLG
                     FQISDVYAGNELPGGAVFIRLNADHHGIALFQATGEQPPGAGLHHIAFEVSTLDEVLR
                     VRDHLRRHDVPIHFEGRRRAGVQLAVEFQDPDGHNLEIYWGIDQIGSDGHVRPPEQWK
                     GARSLEEAITDPVIGQDTTVQDKSLLRA"
     gene            828..1604
                     /locus_tag="VFN42_14015"
     CDS             828..1604
                     /locus_tag="VFN42_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013641090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HET8997780.1"
                     /translation="MGYWGKVIGGVAGFAMGGPFGAVAGAALGHAVDTGATSDFRLPF
                     SGQGLFNPARLTAMLGSRDQLFSVVVVILCAKLAKCDGPVKREEIDAFKRQFRIPPHS
                     VRDIGRLFDQAREDAEGFEPYARQLGQAFLDNRGMLEDVLTALFALARADGPVNRAEQ
                     AFLLRVHHAFGLDDAAWERARGGVPRAAADGPDPYVVLGVPRSAGLEEIRAAWKKLMR
                     ENHPDSLAARGVPAEFINRANEKVARINAAWDRVKRERGN"
     gene            1712..2377
                     /locus_tag="VFN42_14020"
     CDS             1712..2377
                     /locus_tag="VFN42_14020"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET8997781.1"
                     /translation="MLILHYTGMRSGQAAIGRLRDPQARVSSHYVVEEDGTVVRLVPE
                     DRRAWHAGASFWRGHSELNGRSIGIEIVNPGHEWGYRDFPALQMAAVCDLCLEILARH
                     PIPLRNVVAHSDVAPERKEDPGELFDWQGLAQNGVGLWPDVPDAGTGGAVRDAPGLNP
                     VRRALAAIGYRVAPEGALDSPLAAVLRAFQRHWRPEAVTGEADAGTQARLFALLHSIE
                     ATP"
     gene            2702..3622
                     /gene="prpB"
                     /locus_tag="VFN42_14025"
     CDS             2702..3622
                     /gene="prpB"
                     /locus_tag="VFN42_14025"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HET8997782.1"
                     /translation="MTYLVAADLPTAPAGERFGALLARPGILQLPGAQNGQAALQAKQ
                     AGFEALYLSGAAMTASMGLPDLGVITVDEVCFFIRQIARSSGLPLLVDGDTGYGEALN
                     VMHMVRSFEDAGAGAVHIEDQLLPKKCGHLNDKKLADAHDMAAKIAAARKARRHLFII
                     ARTDAAASEGIDGAVARARLYLEAGADAIFPEALNTAEMFRAFAERVPSVPLLANMTE
                     FGRTPFFTASEFQDMGYRMVIWPVSSLRVANKAQAELYAAIRRDGGTQAMVDRMQTRA
                     ELYATIGYHDYEALDSSIVTTVIPTAMPQR"
BASE COUNT          606 a         1287 c         1292 g          571 t
ORIGIN      
        1 gatccaacgc atcttcaaag cgcgtcacga acgcctccca taacaccagt gtccgacgca
       61 tccgtcacct ccagaacagg caacgaacaa agcggacaac tcatgcgcta cgtaaggcgg
      121 acaacaaacg cgctaccgac acgtggcatg gcttgctgta gccccgctgc ggtcgccgca
      181 ttagtctccc tggaaccgag gaggatgcgc caatggatgc agatgccgtc agagccatgg
      241 gtcggccacg tggcctgccc ttcaaactga acaaactcgg ccatatcgca ctctacgtgc
      301 aggacctcca gcgctcggcg cgcttctata ccgaggtgct ggggttccag atctccgacg
      361 tctacgccgg caatgaactc cccggcggtg cggtgttcat ccggctgaac gccgaccatc
      421 atggcatcgc gctgttccag gccaccggcg agcaacctcc gggcgccggc ctgcaccaca
      481 tcgccttcga agtctccacc ctggacgagg tgctgcgcgt gcgagaccac ctccgccggc
      541 acgacgtgcc gatccatttc gagggccgcc gccgcgccgg cgtccagctc gcggtagagt
      601 tccaggaccc ggacgggcac aacctggaaa tctattgggg catcgaccag atcggctcgg
      661 acggccatgt gcgcccgccc gagcagtgga aaggcgcgcg cagcctggag gaggcgatca
      721 ccgatccggt gatcggccag gacaccacgg tgcaggacaa gtcgctgctg cgcgcctgag
      781 cttgccggca ggcgcgagtc gcccggcggg ccgttgaagg aaagcggatg ggatactggg
      841 gcaaagtgat cggcggcgtg gcgggcttcg ccatgggagg acccttcggc gcagtggccg
      901 gtgccgcgct ggggcatgcc gtggataccg gcgccacgtc ggacttccgc ctgccgttca
      961 gcggccaggg cctgttcaac cccgcgcggc tcactgcgat gttgggcagc cgggaccagt
     1021 tgttctccgt cgtcgtggtg atcctgtgcg ccaagctcgc caagtgcgat ggcccggtga
     1081 agcgcgagga gatcgacgcg ttcaagcgcc agttccgcat cccgccgcat tcggtgcgcg
     1141 acatcggccg gctgttcgac caggcgcggg aggacgcgga aggcttcgaa ccctatgcac
     1201 ggcagctcgg ccaggccttc ctggacaatc gcggcatgct ggaagacgtg ctcaccgccc
     1261 tgttcgccct ggcgcgcgcc gacggtccgg tgaaccgcgc ggagcaagcc ttcctgctgc
     1321 gggtccatca tgcgttcggc ctggacgacg cggcctggga gcgcgcccgc ggcggcgtgc
     1381 cgcgtgccgc cgccgacggg ccggacccgt atgtggtgct gggcgtgccg cgctccgccg
     1441 ggctggagga gatccgcgcg gcctggaaga agctgatgcg ggagaaccac ccggacagcc
     1501 tggccgcgcg cggcgtgccg gcggagttca tcaatcgcgc caacgagaaa gtggcgcgga
     1561 tcaatgccgc ctgggaccgg gtcaaacggg aacgcggcaa ttgagcgaag aagaggggcg
     1621 gccggctccc gtgtttgccc ccggccccct gcatatccgc gatctgccga gcccgaactg
     1681 ggatgcgcgg ccggatggtg tgccggtgga catgctgatc ctgcattaca ccgggatgcg
     1741 cagcggccag gcggcgatcg gccggctgcg cgatccgcag gcgcgggtct cctcgcatta
     1801 cgtggtggag gaagacggca cggtggtccg gctggtcccg gaggaccggc gggcctggca
     1861 cgccggcgca tcgttctggc gcggccacag cgagctgaac ggccgctcga tcggcatcga
     1921 gatcgtcaac ccgggccatg aatggggcta ccgcgatttt cccgccttgc agatggcggc
     1981 ggtgtgcgac ctgtgcctgg aaattctggc gcgccatccc atcccgctgc gcaacgtggt
     2041 ggcgcatagc gatgtggcgc cggagcggaa ggaagatccg ggcgagctgt tcgactggca
     2101 gggcctggcg cagaacggcg tcgggctgtg gccggatgtg cccgacgcag gcaccggcgg
     2161 cgcggtacgt gatgcgcccg ggttgaaccc ggtgcgccgc gcgctggcgg cgatcggcta
     2221 ccgcgtggcg ccggaggggg cactggactc gccgctggcc gccgtgctgc gcgcgtttca
     2281 gcgccactgg cgcccggagg cggtcaccgg cgaggcggat gccggcaccc aggcccggct
     2341 gttcgcgctg ctgcacagca tcgaggccac accgtaaacc gctgcagcgg gagggagcgg
     2401 gccagcccct cgcgggactg gcacactccc tacctgcggc gaacgatgat cttgattttg
     2461 atcatcagga tcgtcactat ccgaggtagg agtcggcgac ggtgttgcat cgtcttctcc
     2521 ttccacgcgc cggccgggcc tatcccggcc ggtgtttcgt ttctggcacc gcacggttaa
     2581 gcattgcttg ccggcacgcg ctcgcagaac ccgccgcccg ctgcatccgg aatcaccgtc
     2641 catttccccg cggcggcaag ccggccataa tcgctccgtc cggccattca cggagcccgc
     2701 catgacctat ctcgtcgccg ccgaccttcc caccgccccc gccggagagc gcttcggtgc
     2761 gctgcttgcc cgccccggca tcctgcaact gcccggggcg cagaacggcc aagcggcgct
     2821 acaggcgaag caggctggct tcgaggcgct ctatctctcc ggcgcggcga tgaccgcctc
     2881 gatggggctg cccgacctcg gcgtgatcac ggtggacgag gtgtgcttct tcatccgcca
     2941 gatcgcccga tcttccggcc tgccgctgct ggtcgatggc gacaccggct atggcgaggc
     3001 gctgaacgtg atgcacatgg tgcgcagctt cgaggatgcc ggcgccggcg cggtgcatat
     3061 cgaggaccag ctgctgccga agaaatgcgg ccatctcaac gacaagaagc tggccgacgc
     3121 gcatgacatg gccgccaaga tcgccgccgc ccgcaaggcg cgccggcatc tgttcatcat
     3181 cgcccgcacc gacgccgcgg ccagcgaggg catcgacggc gcggttgccc gtgcgcggct
     3241 gtacctggaa gccggtgccg acgcgatctt cccggaggcg ctgaacaccg ccgagatgtt
     3301 ccgcgccttc gccgagcggg tgccgagcgt gccgctgctg gccaacatga cggagttcgg
     3361 ccgcaccccc ttcttcaccg cgtccgagtt ccaggacatg ggctatagga tggtgatctg
     3421 gccggtcagc tcgctgcgcg tcgccaacaa ggcgcaggcg gagctgtacg ccgcgatcag
     3481 gcgcgacggc ggcacgcagg ccatggtgga ccgcatgcag acccgggcgg agctgtacgc
     3541 cacgatcggc taccatgact acgaggcact ggattcgtcc atcgtcacca cagtgatccc
     3601 gacggcgatg ccgcagcgct gatccgagat gggcgggggc gcaggcccgc cgcccatgat
     3661 gtgctgcggg tgacgcaaag cggcagacca cggctcgaca cactgccctg ccgtggcaca
     3721 gccgccacgg ctcagtcgaa gccgatgaac cccggc
//
LOCUS       DASSZH010000648         5067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_15714, whole genome shotgun sequence.
ACCESSION   DASSZH010000648 DASSZH010000000
VERSION     DASSZH010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15714"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VFN42_14030"
     CDS             <1..244
                     /locus_tag="VFN42_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997783.1"
                     /translation="EWVVFAPLVVLTLWMGIYPPSFSSFWNASVGAMVQHDTAAMAVH
                     RSVAGAPPVVAGLPVVPARITGSAPAMTLKTRSSGL"
     gene            241..1662
                     /gene="nuoN"
                     /locus_tag="VFN42_14035"
     CDS             241..1662
                     /gene="nuoN"
                     /locus_tag="VFN42_14035"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HET8997784.1"
                     /translation="MNWALAAPEIVLACIGMAILLFGVLRKQDNTLICSMLTLGGYLI
                     AGWLAVTRLSGTGYNGLFVVDPFSTFNKVLILAGAALALILSLDWNRKEGISRFEFPV
                     LMLFSVVGMMIMTSAANLMTLYMGLELQSLALYVLAAFARDDIRSSEAGLKYFVLSGL
                     ASGLLLYGISLVYGFSGTMDFGQLRALLVDPAHASTGLVVGIVFVLVGLAFKVSAVPF
                     HMWTPDVYEGAPTPVTVFFATGPKVAAMALFLRVMMISFGHLLGAWQDLIVIVSAASM
                     LLGALAAIGQTNIKRLMAYSSIGHMGYALIGLAAGTQQGIQGVLVYMLVYVFMTVGTF
                     ACIIAMRRRGRAVEQISDLAGLSNTDPALALGFAVFMFSLAGIPVLAGFFAKLYIFLA
                     AVQAGMWGLAVFGVLTSVVGAFYYIRVIKVMYFDAPAPAFDPRAPSLSFVAAITGLFT
                     TFFFLFPAPLIGAAASAAKVLFG"
     gene            1702..2511
                     /locus_tag="VFN42_14040"
     CDS             1702..2511
                     /locus_tag="VFN42_14040"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HET8997785.1"
                     /translation="MLLVVDAGNTNVVFAVREGDAWRGSWRIRTDAQRTSDEYAVWLL
                     SLLTHVGIAPQDISAAVIGTVVPAALYHLRRCCREWFHVEPLVARASLDWGFEIRVDN
                     PDEVGADRLLNALAGHRRFGGPLIVVDFGTATTFDVVDQSGAYLGGVICPGINLSIEA
                     LHQAAARLPRIGIGRPQAVIGRSTVPAMQSGVYWGYIGLIEGLVTRIKAEFGAPMKVI
                     ATGGLAPLFAEGTLMIDQIEPDLTLDGLRMLAERNPAPALPRDRTRLIEHE"
     gene            2709..4355
                     /locus_tag="VFN42_14045"
     CDS             2709..4355
                     /locus_tag="VFN42_14045"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET8997786.1"
                     /translation="MDNGIGDLAFLPLGGTGEIGMNLNLYRCNGRWLAIDCGIGFGGP
                     EHPEVDIMVPDPGYIADRRDRLLGLVITHAHEDHIGAVAHLWPQLRCPVYATPFAAAV
                     LRRKLAEVQLTNQVKVHVIPPGGSIELDPFRLRFIRMSHSIPEAQSLAIETPYGLLLH
                     TGDWKLDPQPLVGPTTDEAALAELGQRGVLAMICDSTNAMVEGHSGSEHEVRTSLCAL
                     IRGIRGRVAVTCFASNVARMESIAYAARECGRSVAVVGRSLRNIDAAARECGYLKGIP
                     PFLGEDDIDDVPDDNILILITGSQGEPRSALARVAMDTHPRVALGEGDTVIFSSRVIP
                     GNERAIGTVQDNLVRRGVHLMTDADHLVHVSGHPARDELRRLYRLVKPRYAVPVHGEW
                     RHLQAHAELAQHCGAVPLMMEDGDILNLAPNQVEVVDSAPVGKLVVDGGRLVPLSGGV
                     MSARRRMLFNGIVVASLAVDEAGRLRGQPRITAPGLFDPDDPETGRIGGEFAEAVADL
                     PVALRRDDASLADAARAALRRSLGRRLQKRPLVDVHLLRV"
     gene            complement(4394..4903)
                     /gene="ccmE"
                     /locus_tag="VFN42_14050"
     CDS             complement(4394..4903)
                     /gene="ccmE"
                     /locus_tag="VFN42_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010501009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HET8997787.1"
                     /translation="MTRKRRRLWIVVACGIGLGSATALTLSAFSSNLVFFVSPSDLIH
                     KPDLGRTVRLGGLVQQGSVHHSQINGQPAATFDVTDGNNAVKVTYVGILPDLFRQGQG
                     VVVLGAMEPNGVFHASEVLAKHSENYMPKEVEAALKKSGHWNPNAGPPPAASTWDTMS
                     VKNATKAGS"
BASE COUNT          751 a         1659 c         1701 g          956 t
ORIGIN      
        1 tgaatgggtg gtgttcgcgc cgctggtggt gctgacgctt tggatgggca tctatccgcc
       61 cagcttctcc agcttctgga atgccagcgt cggcgcgatg gtgcagcatg ataccgctgc
      121 gatggcggtg catcgttccg tcgccggcgc accgcctgtt gtcgccggcc ttccagtcgt
      181 tccggcgcgg atcaccggct ctgccccggc catgacactg aagactcgga gttctggact
      241 atgaactggg cccttgccgc tccggagatc gtccttgcct gcatcggcat ggccatcctg
      301 ctattcggcg tcctgcgcaa gcaggacaac acgctgatct gcagcatgct gacgctgggc
      361 ggctacctga ttgccggctg gcttgcggtg acccggctgt ccggcaccgg ttataacggg
      421 ctgttcgtcg tggatccttt cagcaccttc aacaaggtgc tgatccttgc cggcgcggca
      481 ttggcgctga tcctgtcgct ggactggaac cgcaaggaag gcatttcccg cttcgagttc
      541 ccggtgctga tgctattcag cgtcgtcggc atgatgatca tgacatcggc cgccaacctg
      601 atgacgttgt acatgggttt ggaactgcag tccctggcgc tctacgtgct ggcggccttt
      661 gcccgtgacg acatccgttc ctcggaagcc ggtctgaagt atttcgtgct gtccggcctg
      721 gcgtcgggcc tgctgctgta cggcatttcg ctcgtatacg gcttctccgg cacgatggat
      781 ttcgggcaac tgcgcgcctt gcttgtggat cccgcgcacg cctccaccgg cctcgtcgtc
      841 ggtatcgtgt tcgtgctggt cgggctcgcc ttcaaggttt ccgccgtgcc gttccacatg
      901 tggacgccgg acgtctatga gggcgcgcca acgcccgtga cggtgttctt tgccaccggc
      961 ccgaaagttg cggcgatggc gctgttcctg cgcgtcatga tgatctcctt cgggcatctg
     1021 ctcggcgcat ggcaggacct gatcgtcatc gtgtccgccg catccatgct gttgggcgca
     1081 ttggcggcca ttggccagac caacatcaag cggctgatgg catactcctc gatcggtcac
     1141 atgggctatg cgctgatcgg cctcgccgcg ggaacccagc agggcatcca gggcgtgctg
     1201 gtctacatgc tggtctatgt cttcatgaca gtcggcacct tcgcctgcat catcgccatg
     1261 cgccgccgcg gcagagcagt ggagcagatc agcgatctgg caggattgtc gaataccgat
     1321 ccggcgctgg cgctcggctt tgcggtgttc atgttcagcc tggcaggaat tccggtgctc
     1381 gccggcttct tcgccaagct ctacatcttc ctggcggcag tgcaggccgg tatgtggggg
     1441 ctggcggtgt tcggcgtgct gaccagcgtg gtgggcgcat tctactacat ccgcgtcatc
     1501 aaggtgatgt acttcgatgc gccggcgccg gcattcgatc cgcgtgcgcc ctctctgtcg
     1561 ttcgtcgccg cgatcaccgg gctgttcacc accttcttct tcctgttccc ggcgccgttg
     1621 attggcgccg cggcctcggc agcgaaggtt ctgttcgggt gaccatcgcc agcgcggacc
     1681 ggccgtgaca aggttgtgcc catgctgctc gtcgtagatg ccggtaacac caatgtcgtg
     1741 ttcgccgtgc gcgaggggga cgcatggcgc ggctcctggc ggatccgcac cgacgcgcaa
     1801 cgcacgtcgg acgaatacgc cgtctggctg ttgtcgctgc tgacccatgt cggcatcgcg
     1861 ccgcaggaca tctccgccgc ggtcatcggc actgtcgttc cggccgcgct gtaccacctt
     1921 cgccgatgct gccgcgaatg gttccatgtg gagccgctgg tggcgcgcgc cagcctggac
     1981 tgggggttcg agatccgggt ggacaacccg gatgaggtcg gcgccgaccg tctgctcaac
     2041 gcgctcgccg gccatcggcg ttttggcgga ccgctgatcg tggtggattt cggcaccgcc
     2101 actaccttcg atgtggtgga ccagtccggt gcctatctgg gcggtgtcat ctgtcccggc
     2161 atcaacctct ccatcgaggc gctgcaccag gccgcagccc gcctgccacg catcggcata
     2221 ggccggcctc aggcggtgat cggccgatcc accgtgcccg ccatgcagtc cggcgtgtac
     2281 tggggttata tcggcttgat cgagggcctg gtcactcgca tcaaggccga gtttggcgcc
     2341 ccgatgaagg tgatcgccac cggcgggctc gcgcccctgt tcgccgaggg cacgctgatg
     2401 atagaccaga tcgagcccga cctgaccctg gacggcttgc gcatgctggc cgaacgcaac
     2461 ccggcaccgg ctttgccgcg ggatcgcaca aggctgatcg agcacgagtg agtcgcttgc
     2521 cctgctgcgg ctgatctgat gcgtgccgga ggcggcattc tgcggtttgc gccgggtggg
     2581 caccgcctga aaccggcgcg acgtcgctgg tatcgccgca cggattaccc catatccacc
     2641 gccaatatgc ctcgcccggc gaggcacccg atcctgctca gcaacaaatg aggtaagcag
     2701 cacaccgtat ggataatgga atcggagatc tggccttcct gccgttgggc gggacgggtg
     2761 agatcggcat gaatctcaac ctctaccggt gcaatggccg gtggctggcg attgactgcg
     2821 ggatcgggtt cggcgggccg gaacatcccg aggtcgatat catggtccct gacccgggct
     2881 atattgccga ccgccgcgac cggctgctgg gtctggtaat cacccacgcg catgaggacc
     2941 acatcggtgc ggtggcgcat ctctggccgc aactgcgctg ccctgtgtat gctacgccct
     3001 tcgccgccgc cgtgctgcgc cgcaagctgg ccgaggtgca gctgaccaac caggtgaagg
     3061 tccacgtcat cccgcccggc ggcagtatcg agctcgatcc gttcaggctg cgctttatcc
     3121 gcatgtcgca ctccatcccg gaggcgcagt cgctggcgat cgaaacgccg tacggcctgc
     3181 tgctgcacac cggcgactgg aaactggacc cgcagccgct ggtcggcccg accacggacg
     3241 aagcggcgct ggcggaactc ggccagcgcg gcgtgctggc gatgatctgc gattccacca
     3301 acgccatggt ggagggccat tccggcagcg aacacgaggt ccgcaccagc ctgtgcgcgc
     3361 tgatccgcgg cattcgcggc cgcgtcgcgg tgacctgctt cgccagcaac gtcgcgcgga
     3421 tggagagcat cgcctacgcc gcccgcgagt gtggccgcag cgtcgctgtg gtcggccgct
     3481 cgctgcgcaa tatcgatgcc gctgcgcgcg agtgcggcta cctgaagggc atcccgccgt
     3541 tcctcggcga ggacgatatc gacgacgttc ccgacgacaa catcctgatc ctgatcaccg
     3601 gcagccaggg ggagccgcgc agcgcgctgg cccgcgtggc gatggatacg catccgcgcg
     3661 tggcgttggg ggagggcgac acggtgatct tctcctcccg tgtcattcca ggcaatgaac
     3721 gcgcgatcgg cacggtgcag gacaacctgg tgcgtcgcgg cgtgcatctg atgaccgatg
     3781 ccgaccacct ggtgcacgtc tccggccatc ccgcacgcga cgagctgcgc cggctgtacc
     3841 ggctggtgaa gccgcgctac gccgtcccgg tgcacgggga atggcggcac ctgcaggcgc
     3901 atgccgaact agcgcagcac tgtggcgccg tgccgctgat gatggaggac ggcgacatcc
     3961 tcaacctggc gccgaatcag gtggaggtcg tggacagcgc gcccgtcggc aagctggtgg
     4021 tggatggcgg gcggttggtg ccgctgagcg gcggcgtgat gtctgcccgc cgccgcatgc
     4081 tgttcaacgg catcgtggtg gccagcctgg cggtggacga agccggccgg ctgcgcggcc
     4141 agccgcgcat cacggcgccc gggctgttcg acccggacga tccggaaacc gggcggatcg
     4201 ggggagaatt cgccgaagct gtggccgacc tgcccgtggc gctgcggcga gacgatgcca
     4261 gcctggccga tgccgcccgg gctgccctgc gccggtcact ggggcggcgg ctacagaagc
     4321 gccccttggt ggacgtgcat ctgctgcggg tctgaagtcg ggacctcctc cgccgtgcca
     4381 gcggcggagg gtgtcagctg ccggccttgg tggcgttctt caccgacatt gtgtcccagg
     4441 tggacgcggc ggggggcggg ccggcattcg ggttccagtg gccggacttc ttcagcgccg
     4501 cctcgacctc cttcggcatg tagttctccg aatgcttggc cagcacttcg gaggcatgga
     4561 acaccccgtt cggctccatg gcgcccagca ccaccacgcc ctggccctgg cggaacaggt
     4621 cgggaaggat gcccacatag gtcaccttca ccgcgttgtt gccgtcggtc acgtcgaacg
     4681 tggccgccgg ctgcccattg atctgcgaat gatgtacgct gccctgttgc accagcccgc
     4741 ccaggcgcac ggtgcgcccc aggtccggct tgtggatcag gtcggacggg gagacgaaga
     4801 acaccaggtt gctgctgaag gcggacaagg tcagcgcggt ggcggagccc aggccgatcc
     4861 cacaggcaac gacaatccac aggcggcggc gtttgcgggt catgcgagca ccagggacag
     4921 atcgtgacga gcggtcatcc ggccctccgc tgctgccgcg gatcgaccac ggcgagcttg
     4981 cggcgggcgg cgcccatgcg catccaggcg gcgatgccga agccggcggg aagcaagacc
     5041 gccagtgcat aggacgcggc gatgtag
//
LOCUS       DASSZH010000649         4404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_987459, whole genome shotgun sequence.
ACCESSION   DASSZH010000649 DASSZH010000000
VERSION     DASSZH010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987459"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VFN42_14055"
     CDS             complement(<1..293)
                     /locus_tag="VFN42_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997788.1"
                     /translation="MTARLGAIFGGNAVLFSQDSLSPAAFVFETAGFENRFVASYADH
                     YAATNVWTPGVAVQPDGAIVTGDEIIGRREFEQTEWYNDWLRPQGLYHGTGAVL"
     gene            complement(361..648)
                     /locus_tag="VFN42_14060"
     CDS             complement(361..648)
                     /locus_tag="VFN42_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997789.1"
                     /translation="MLCQAWPSAVTEYTGTAGAGAAASPNATALGSRQRIEEDCRSRM
                     RGLTLRHGAFCYVSGPIFILRAYAPRIAAAMRFDRSLHACLFWQANENTDA"
     gene            complement(1124..1939)
                     /locus_tag="VFN42_14065"
     CDS             complement(1124..1939)
                     /locus_tag="VFN42_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HET8997790.1"
                     /translation="MRILFIGDVVGRSGRDAVAAALPQLRQALRIDLAVVNAENASHG
                     FGLSPEMAVALFTAGADVITLGNHSWDRKEIIPYIAGAPRLIRPLNYPPGTPGSGAAL
                     VEVPGGRRALVLQAMGRLYMDPLDCPFRGTSAELAKHRLGVTVAAIVVDIHAEASSEK
                     MAFAHFLDGTVSLVVGTHTHCPSADAQVLPGGTAFQSDAGMSGDYDSVIGMQKDAATL
                     RFWRKMPGERLAPAEGEATVCGVFVETDDATGLARRVAPVRCGGRLAPAMPVV"
     gene            complement(1940..2521)
                     /locus_tag="VFN42_14070"
     CDS             complement(1940..2521)
                     /locus_tag="VFN42_14070"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HET8997791.1"
                     /translation="MAAPHIMSDLDEAKRALRARALALRAGCDPAASGAALTEHVLAA
                     ACAPPAGAVVSGFWPIGEEIDIRPLLHRLHARGHTVALPVTPKRGNPLSFRTWQPGDV
                     LQPERFGTFRPVGQDVVPDFLLVPLLAFDRRGYRVGYGGGFYDRTLAALPHAFALGVA
                     YAAQQVDAVPTGPHDKRLNAVATEDGVIFCKDA"
     gene            complement(2508..2664)
                     /gene="ssrS"
                     /locus_tag="VFN42_14075"
     ncRNA           complement(2508..2664)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VFN42_14075"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(2675..3004)
                     /locus_tag="VFN42_14080"
     CDS             complement(2675..3004)
                     /locus_tag="VFN42_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010666544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HET8997792.1"
                     /translation="MAQVTLRINGYAYTVGCRDGEEQHLQAMAAQVERRIDKVKATAG
                     QSGEARMLVMASLLMADDLFEMEQRVRTAEAAAAGAPPGNDAKLGRRLNRMARRAEEI
                     AAGLEAH"
     gene            complement(3031..3249)
                     /locus_tag="VFN42_14085"
     CDS             complement(3031..3249)
                     /locus_tag="VFN42_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997793.1"
                     /translation="MASESDDAAEAAARLEAALERIAALAGSQPKPHADGADSGEAAE
                     PAAPVEEIAARLDSLIDRLRAALGLGHD"
     gene            3311..3739
                     /locus_tag="VFN42_14090"
     CDS             3311..3739
                     /locus_tag="VFN42_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997794.1"
                     /translation="MRLPPAVIVHGLADACTVLAAGAPVTLLSAPGAAEYAGCSWWQA
                     LLAQAGKNCPGASFTDLLDCGEASGYALAALRIGLVRLVLRPGAPGWNGVAAIAARQG
                     GMLLDAPPPALDMAERRARRRLHDWLHARTTPGDSGPALS"
     gene            3782..>4404
                     /locus_tag="VFN42_14095"
     CDS             3782..>4404
                     /locus_tag="VFN42_14095"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003624754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HET8997795.1"
                     /translation="MRVTQKVKKILDWYEGDPPGVKANLARILCTGKLGGTGKVVILP
                     VDQGFEHGPARSFAPNPAAYDPHYHYSLAIEAGLNAYAAPLGMIAAGADTFAGQIPTI
                     LKMNSSNSLASTKDQAVTASVNDALRLGCAAIGFTIYPGSEYAFDQMEELRELTEEAK
                     AAGLAVVVWSYPRGPNLDKAGETALDICAYAAHMAALMGANIIKVKPP"
BASE COUNT          709 a         1553 c         1467 g          675 t
ORIGIN      
        1 agcacggcgc cggtgccgtg gtacaggcct tgcggccgca accagtcatt gtaccactcg
       61 gtctgctcga attcccggcg gccgatgatc tcgtcgccgg tgacgattgc gccgtccggc
      121 tgcaccgcga cgccgggcgt ccagacattc gttgcagcgt agtgatcagc gtagctggcg
      181 acgaagcggt tctcgaagcc tgcggtttcg aacacgaagg cagccgggct aaggctgtcc
      241 tgcgagaaca ggaccgcgtt gccgccgaag atcgccccca gtcttgcggt caatggacgc
      301 catcgactcg cgtcgagagc agcttcgtaa atgtcgtcca ggatggaggt gagctcggct
      361 tcaggcgtcg gtgttttcat tggcctgcca gaataagcaa gcgtgcagcg accggtcaaa
      421 tcgcattgcc gcggcgatgc gcggtgcata agcgcgtaat atgaaaatcg gacccgaaac
      481 gtagcaaaac gctccgtgcc gcagcgtcag ccctcgcatc cggctgcggc agtcttcctc
      541 gatgcgttgc ctgctgccca gtgcggtggc gttcgggctg gcagcagcgc cggcgccagc
      601 ggtgccggtg tattcggtga ccgcactcgg ccacgcttgg cagagcatcc agctatccca
      661 atggcctcag cgcgaccggt gccgtaaccg gcaatgcaag cacctccacc gcgttgcatg
      721 cgttcctctg gaccaatgct ggcggcatgc gggatctggg gcaggcgatg tcaccggaag
      781 cgcagtcacc gcgggcggcg ccacccacgc cttccggtgg agcggcggga cgatggacga
      841 cctgaacgat ctgatcagcc caggcgatcc gctataccgc accatcagct tcaccaacgg
      901 tcactgaatc aacgacacag gtcagatcat cgccgagggg aacaacagcc aagcctacct
      961 gctgacaccc gtcgcggcgg ttcccgaacc ggcgtcattc ggctggttgg cgtgcggcac
     1021 ggcggggctc gccctgatcc ggcgcgcgca ccgtttgtgc gatggcggag gcgcatgatc
     1081 ccggcacaag ccgacgcggc tcgggccaag gcccgggcag ctgtcacacc accggcatcg
     1141 ccggcgccag ccggccgccg cagcgcaccg gcgcgacgcg ccgcgccaat ccggtggcgt
     1201 cgtccgtttc cacgaacacg ccgcacaccg tcgcctcgcc ttccgccggc gccagccgct
     1261 cgcctggcat cttgcgccag aagcgcagcg tggccgcatc tttctgcatg ccgatcacgc
     1321 tgtcataatc gcccgacatg cccgcatccg actggaacgc cgtgccgccc ggcagcacct
     1381 gcgcgtcggc ggacggacaa tgcgtgtgcg tgcccaccac cagcgacacc gtgccgtcca
     1441 ggaaatgcgc gaaggccatc ttctcactgg acgcctcggc atgaatatca accacgatcg
     1501 ccgccaccgt cacccccagc cggtgtttcg ccagctcggc cgaggttccg cggaatgggc
     1561 agtccagcgg gtccatgtac agccgcccca tcgcctgcag caccagcgcc cgccggccgc
     1621 ccggcacctc caccagcgcg gcgccgctgc ccggtgtgcc gggcggatag ttcagcgggc
     1681 ggatcagccg gggtgcgccg gcgatgtagg ggatgatctc cttgcggtcc caactgtgat
     1741 tgcccagggt gatcacgtcg gcgccggcgg tgaacagcgc caccgccatc tccgggctca
     1801 ggccgaagcc gtgcgacgcg ttttccgcat tcaccaccgc aaggtcgatg cgcaacgcct
     1861 gccgcagttg cggcagcgcc gccgccaccg cgtcccggcc gctgcggccc accacgtcgc
     1921 caatgaacag aatccgcatt cacgcgtcct tgcagaagat cacgccgtcc tcggtggcga
     1981 cggcattcag ccgcttatcg tgcggcccgg tgggcaccgc gtccacctgt tgcgccgcgt
     2041 aagcgacgcc gagcgcgaag gcgtgcggca gcgctgccag ggtacggtcg tagaacccgc
     2101 cgccatagcc cacccggtag ccgcgccggt caaacgccag cagcggtacc agcaggaagt
     2161 ccggcaccac atcctgcccc actgggcgga aggtgccgaa gcgctccggt tgcagcacgt
     2221 cgcccggctg ccaggtccgg aaactgagcg gattgccgcg cttgggcgtc accggcagcg
     2281 ccacggtatg gccgcgcgca tgcagccgat gcagcagggg acggatatcg atctcctcgc
     2341 cgatcggcca gaagccggac acgaccgcgc ctgccggcgg ggcgcatgcc gccgccagca
     2401 cgtgctcggt cagcgcggcg ccggaggcgg ccggatcgca gccggcgcgc aacgccagcg
     2461 cacgcgcccg cagcgcccgc ttggcttcgt ccagatcgga catgatgtgc ggagccgcca
     2521 tggccgttgg cgtctcatcc tcccaaggcc tgcgtgagca ggtgggcgcc ggataccaag
     2581 accacgatcc cagcagagcc agctcccgag gaggatgctt tgtggccccg gggatgaatt
     2641 gcctgacgca ccgggcagcc ccgcccgacg atacttagtg tgcctccagc cccgcggcaa
     2701 tctcttccgc ccgccgtgcc atccgattca gccggcggcc gagcttggcg tcattgccgg
     2761 gcggggcgcc ggcggcggcg gcttcggcgg tcctgacccg ctgttccatc tcgaacagat
     2821 cgtccgccat cagcagcgag gccatcacca gcatgcgggc ttccccgctt tgcccggcgg
     2881 tggccttgac cttgtcgatg cgtcgttcca cctgcgcggc cattgcctgc aggtgctgtt
     2941 cctcgccgtc gcggcagcca accgtgtagg cgtagccgtt gatgcggagc gtcacttgcg
     3001 ccatcgatgc gaatcccttg cgtgcgacgg tcagtcgtgc cccaagccga gggcggcgcg
     3061 caatctatca atcaagctgt cgagtcgggc ggcaatttcc tccaccggcg cggccggttc
     3121 cgccgcctca ccggagtccg cgccgtccgc atggggtttc ggctgcgaac cggccagcgc
     3181 ggcgatgcgc tccagggcgg cttccagccg cgccgcggcc tcggccgcgt cgtcgctttc
     3241 gctcgccatg tcgcgctgcc ctcctgaatg acgcttgcac tgaaagcggc agcaagcttc
     3301 aaggtcaagc atgaggctgc cgcctgccgt cattgtgcac ggacttgccg acgcctgcac
     3361 agtgctggcg gcgggcgcgc ccgtcacgct gctgtcggcg ccgggcgcgg cggaatacgc
     3421 cggatgcagc tggtggcagg cgctgctggc gcaggccggc aagaactgtc cgggcgccag
     3481 cttcaccgat ctgctggatt gcggcgaggc gtctggctac gcgctggcgg cgctgcgcat
     3541 cggcctggtg cgcctggtgc tgcgtcccgg cgcgcctggc tggaatgggg tggcggcgat
     3601 cgccgcgcga cagggcggca tgctgctgga cgcgccgccg cctgccctgg atatggcgga
     3661 gcggcgcgcg cgccggcggt tacacgattg gttgcacgcg cgaaccaccc cgggtgacag
     3721 cggcccggcc ctgagctagt agcgttgata accagccaat ttgagagaag ggacaggccc
     3781 gatgcgcgtc acccagaagg tgaaaaagat cctggactgg tatgagggcg atccgcccgg
     3841 cgtgaaggcc aacctcgcgc gcatcctgtg caccggcaag ctcggcggca ccggcaaggt
     3901 ggtgatcctg ccggtggacc aaggctttga gcacgggccg gcgcgcagtt tcgcccccaa
     3961 tccggcggcc tatgacccgc actaccacta ctcgctggcg atcgaggccg ggctgaacgc
     4021 ctatgccgcg ccgctgggca tgatcgccgc cggtgccgac accttcgccg ggcagatccc
     4081 gaccattctg aagatgaaca gcagcaactc gctggccagc accaaggacc aggcggtgac
     4141 tgccagcgtc aacgacgcgc tgcggctggg ctgcgcggcg atcggcttca ccatctatcc
     4201 cggcagcgaa tacgccttcg accagatgga ggaactgcgc gagctgaccg aggaagccaa
     4261 ggccgccggc ctggccgtgg tggtgtggag ctatccgcgc gggccgaacc tggacaaggc
     4321 gggcgagact gcgttggaca tctgcgccta tgccgcgcac atggcggcgc tgatgggcgc
     4381 caacatcatc aaggtgaagc cgcc
//
LOCUS       DASSZH010000650         3269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1277091, whole genome shotgun sequence.
ACCESSION   DASSZH010000650 DASSZH010000000
VERSION     DASSZH010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277091"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(477..1382)
                     /gene="speB"
                     /locus_tag="VFN42_14100"
     CDS             complement(477..1382)
                     /gene="speB"
                     /locus_tag="VFN42_14100"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HET8997796.1"
                     /translation="MAGTPTFATPPSFLGVPPRAGTAAFTVAGIPLDLGTTNRSGARD
                     GPHAIRRASRMLVDGAHPEFWVEPAALDLADIGDFSLALGDTAGSLALIEQQAARIPH
                     LLALGGEHGITLPLLRALHRRIGNPLALVHFDAHVDTWPDNFGQRYAHGSVFYHAIEE
                     DLVDPHRMVQIGIRSPVQRDVWDWTLDHGVTVLTAQQVHAAGADGIAQQVRAVVGDAP
                     AYLSFDVDALDPAFAPGTGTPEIGGLASWQAQAVLRRLRGLPFVGMDVVEVAPAYDVS
                     EITALAAATVAWEYLALLGAAEPGR"
     gene            complement(1564..2727)
                     /locus_tag="VFN42_14105"
     CDS             complement(1564..2727)
                     /locus_tag="VFN42_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HET8997797.1"
                     /translation="MRILDVREVTKPISSPIRNAYIDFSKMTSSLVAVVTNVERNGKQ
                     VVGYGFNSNGRYGQGGLIRERFAGRIMEADPASLLDATGDNLDPDRIWACMMQNEKPG
                     GHGDRSVAVGTIDMAVWDAVAKIAGKPLFRLLAERHGREADPRVFVYAAGGYYYPGKD
                     DAALCAEMRSYLERGYSVVKMKIGGESIATDRRRIEAVLREIGETAQLAVDANGRFDL
                     ETAIAYAKMLRDYPLFWYEEAGDPLDYQLQAAVAEFYPGPMATGENLFSHQDARNLIR
                     YGGMRPDRDWLQFDCALSYGLCEYQRTLQMLKDAGWSWTRCIPHGGHQMSLAIAAGLG
                     LGGNESYPDVFQPYGGFPDGTRVQDSIVTLPDLPGIGFEGKSDLFSEMRALTA"
     gene            2833..>3269
                     /locus_tag="VFN42_14110"
     CDS             2833..>3269
                     /locus_tag="VFN42_14110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8997798.1"
                     /translation="MSPPLVAAILPPHEGFGPGQAGAIGMVARAQAQTPGFRAVVFGG
                     PQSRVFGDVAFRAVRPAWWFPGNVNLRFGAALLRPLRRLRPALVEVHNRPELALFLAR
                     WLPRTRVALFLHNDPQSMRRARTPAERTVLLRRLAGVVTVSEYL"
BASE COUNT          503 a         1128 c         1139 g          499 t
ORIGIN      
        1 gaccagctgg tcgacggtcg gcaggctggc cggacgggga tcgaagcggc gacgcaatgg
       61 agacacggcc gcacgggcgc gcagtggggt catgctggaa acctcgcagg caccgccgga
      121 cgcggcttgg gcgcacccgg cacacaggac ggaacggggg aaagccggga cggcgcgttg
      181 cgccggtccg gcggtgaatc agccgctgct gcgaggtttt cgcatcgcgg ctaagcgctc
      241 agccaacata ggcggctccc taacgacccc ggcccacggt gtggccggcc cgaccgaaaa
      301 ggacgcgtta cgtcgttgct gtgcccgggc ggagtgcgcc ggggggctcg cggcacgtgc
      361 gatgcgtcaa accgcagcgc cccgagggac gctgcggaga gcggcatata atagggttga
      421 gcggggaacg ccatgcgttt ttgcgaggcg agcaaattga tgggcgcatg cagccttcaa
      481 cggcccggct ccgccgcacc gagcagcgcc agatactccc aggcgaccgt cgccgcagcg
      541 agcgcggtga tctccgacac atcgtaagcc ggggcgacct ccaccacgtc catgccgaca
      601 aacggcaggc cgcgcagccg ccgcaggacc gcctgtgcct gccacgacgc cagcccgccg
      661 atctccggcg tgccggtgcc gggagcgaag gcgggatcga gcgcatccac gtcaaagctc
      721 agataggccg gcgcgtcgcc gaccaccgcg cggacctgct gcgcgatccc gtcggcgccg
      781 gcggcgtgca cctgttgcgc cgtcagcacc gtgacgccat ggtcgagcgt ccagtcccag
      841 acatcgcgct gcaccgggga gcggatgccg atctgcacca tgcggtgcgg atcgaccagg
      901 tcttcctcga tggcgtggta gaacaccgaa ccatgtgcat agcgctgacc gaaattgtcc
      961 ggccaggtgt ccacgtgcgc gtcgaaatgc accagcgcga gcgggttgcc gatccggcga
     1021 tgcagggcgc gcagcaacgg cagggtgatc ccgtgttcgc cgcccagcgc gaggagatgc
     1081 ggaatgcgcg cggcctgctg ctcgatcagt gccaggctgc cggctgtgtc gccaagtgcg
     1141 aggctgaagt cgccgatatc cgccaggtcg agtgcggccg gctcgaccca gaactccggg
     1201 tgcgcgccgt ccaccagcat gcggctggcg cgccggatgg cgtgcgggcc atcgcgtgcg
     1261 ccagagcggt tcgtcgtgcc gaggtcgagc gggatgccag cgacggtgaa ggctgccgtg
     1321 ccggccctgg gcggaacgcc caggaagctg ggcggcgtgg cgaaggtggg cgtgccggcc
     1381 atgtgcaaac tcccgatgcg gcgtgcggtg tgcgccggct cggcgccctc cgtcatggcc
     1441 ggcgaaggtc ggccatccac gtcttgcccc ggggcacgcg cagccgcaaa cgaatcagct
     1501 gccaggaaat ccccgcagca aggcatggac ggccggcctt cgccggcgat gctggctgcg
     1561 ccgttacgcc gtcagcgcgc gcatctcgct gaacaggtcc gatttcccct cgaatccgat
     1621 gcccggcaag tcgggcaggg tgacgatcga gtcctgcacg cgcgtgccgt ccgggaaccc
     1681 gccatagggc tggaacacgt ccgggtaact ctcattgccg cccaacccga gccccgcggc
     1741 aatggccagc gacatctgat gcccgccatg cggaatgcag cgcgtccagc tccagccggc
     1801 atccttcagc atctgcagcg tgcgctggta ttcgcataag ccgtaggaca gcgcgcagtc
     1861 gaattgcagc cagtcgcgat ccggccgcat gccgccgtag cggatcaggt tgcgcgcatc
     1921 ctggtggctg aacaggtttt cgccggtcgc catcggcccc ggatagaatt ccgccactgc
     1981 ggcctgcagc tgatagtcca gcggatcgcc ggcttcctcg taccagaaca gcggataatc
     2041 ccgcagcatc ttcgcatagg cgatggcggt ttccaggtcg aagcggccgt tggcgtccac
     2101 cgccaactgc gcggtctcgc cgatctcccg cagcaccgcc tcgatgcggc ggcggtcggt
     2161 ggcgatgctt tcgccgccga tcttcatctt cacgacgctg tagccgcgct ccaggtagct
     2221 gcgcatctcg gcgcacagcg cggcgtcgtc cttgccgggg tagtagtaac cgccggccgc
     2281 atagacgaac acccgcggat ccgcctcccg cccgtgccgc tcggccagca ggcggaacag
     2341 cggcttgccg gcgatcttcg ccaccgcgtc ccacaccgcc atatcgatcg tgcccaccgc
     2401 gaccgagcgg tcgccgtggc cgccgggctt ctcgttctgc atcatgcagg cccagatgcg
     2461 gtcgggatcg agattgtccc cggtggcatc caatagcgac gccgggtccg cttccatgat
     2521 ccggccggcg aagcgctcgc ggatcagccc gccctggccg tagcggccgt tcgaattgaa
     2581 gccgtatccc accacctgct tgccgttgcg ctccacattg gtgaccaccg cgaccaggct
     2641 gctggtcatc ttgctgaagt cgatataggc gttgcggatc ggcgagctga tcggcttggt
     2701 gacctcgcga acgtcgagga tgcggatggg catgcagcag tctccggcac tggacgagcg
     2761 ggttttccgc cggaaccgca gccggtgcaa cgttccttct ccatgccgtc gggcggagag
     2821 ggaagcgaag cggtgagccc gccgctggtc gcggctatcc tgccgccgca cgaaggcttc
     2881 ggacccggcc aggcgggcgc gatcggcatg gtggcgcgtg cccaggcgca gacgcccggc
     2941 ttccgcgccg tggtgttcgg cggcccgcaa tcgcgcgtgt tcggcgacgt ggcgtttcgc
     3001 gcggtgcggc cggcctggtg gtttcccggc aacgtcaacc tgcgcttcgg cgcggcgctg
     3061 ctgcggccgc tgcggcgact gcgccccgca ctggtcgagg tgcacaaccg gccggaactg
     3121 gcgctgttcc tggcgcgctg gctgccgcgg acccgggtgg cgctgttcct gcacaacgac
     3181 ccgcaatcca tgcgccgcgc ccgcacaccg gccgagcgta ctgtgctgtt gcggcgcctg
     3241 gcgggggtgg tcaccgtgtc cgaatatct
//
LOCUS       DASSZH010000651         2755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1618248, whole genome shotgun sequence.
ACCESSION   DASSZH010000651 DASSZH010000000
VERSION     DASSZH010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618248"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VFN42_14115"
     CDS             <1..665
                     /locus_tag="VFN42_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8997799.1"
                     /translation="GFGSDMTGDKAGNLAAFCAERGQAMLRFDYSGHGTSGGRFEDGS
                     IGTWAADTLCAIDRLTEGPLVLVGSSMGGWIALLTALARPQRIAALVGIAAAPDFTQA
                     LMWQRFSFEQRATLMQSGVLHLQSQYGGPTLITRRLIEDGRDHLLLTGPIALDCPVRL
                     LHGQVDPDVPWEMALTIAEQITGADVQVTLVKDGNHRLSRPQDLALLRRVLGPLLGQD
                     GA"
     gene            complement(621..1466)
                     /locus_tag="VFN42_14120"
     CDS             complement(621..1466)
                     /locus_tag="VFN42_14120"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8997800.1"
                     /translation="MMLLIFGLGYTGRAVAALARANGIAVCGTSRESAADGGIAFQQA
                     GEAVAQATHLLSTAPPDASGDPVLARYAAQIEAAPALRWIGYLSSTVVYGDRAGGWVD
                     EDTPPQPSQERGQRRLDAELAWSRFAGRRAVDVFRLAGIYGPGRSAFDDLRAGRARRI
                     AKPGHAFGRIHRDDITRAVLAAMRQQRPPGTRILNLNDDEPAETSAVIAEAAHLLGIA
                     PPTEIPFAQALPGMSPMARSFWVENRKVASRKTQAALGISWRYPTYREGLRAILAQQR
                     AKDPA"
     gene            1643..2497
                     /locus_tag="VFN42_14125"
     CDS             1643..2497
                     /locus_tag="VFN42_14125"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET8997801.1"
                     /translation="MQSWRIGDVTVSKLVELEMTGGSRFLLPQATPEEIKPIGWLQPH
                     FADENGKLRMSIHSFIVQTPTRRIIVDTCLGNDKQQRRIPHWNDRQGPFLTDLAAAGF
                     PRESIDTVLCTHLHVDHVGWNTMKVDGKWVPTFPQARYLMGRSEYEHWRDNTAREDMR
                     HILADSVTPILDAGLAELVETDHQLTDEISLVPTLGHTPGHVSVRIVSRGEEALITGD
                     FMHHPCQIAHPEWSTLADSDPEQGIRTRWRMFEQLAGKPVLVIGTHFAGVTAGRLVRD
                     GATFRLAV"
BASE COUNT          474 a          943 c          938 g          400 t
ORIGIN      
        1 ccggcttcgg cagcgacatg accggcgaca aggccggtaa cctcgccgcg ttctgcgccg
       61 agcgggggca ggccatgctg cggttcgatt attccggaca tggcaccagc gggggaaggt
      121 tcgaggacgg cagcatcggc acctgggccg ccgacacgct atgcgcgatc gaccggctga
      181 ccgaaggacc gttggtgctg gtcggttcgt cgatgggcgg ctggatcgcg ttgctgaccg
      241 cgctcgcccg accgcagcgc atcgccgcac tggtcggcat cgccgcggcg ccggacttca
      301 cccaggcgtt gatgtggcaa cgcttcagtt tcgagcagcg cgccacgctg atgcaaagcg
      361 gcgtgctgca cctgcaaagc caatacggcg gcccaaccct gatcacgcgc cggctgatcg
      421 aggatggccg cgaccacctg ctgctgaccg ggccgatcgc gctggactgc ccggtgcggc
      481 tgctgcatgg ccaggtcgat cccgacgtgc cctgggagat ggcgctgacc atcgcggaac
      541 agatcaccgg cgccgacgtg caggtgaccc tggtgaagga cggcaaccac cgcctgtcgc
      601 ggccgcagga ccttgcgctg ctacgccggg tccttggccc gctgctgggc caggatggcg
      661 cgtagcccct cgcggtaggt ggggtaccgc caggagatcc ccagcgccgc ctgggtcttc
      721 cggctggcaa ccttgcggtt ctccacccag aagctgcgcg ccatcggcga catgccgggc
      781 agcgcctgcg cgaagggaat ctccgttggc ggcgcgatgc ccagcagatg cgcggcttcc
      841 gcgatcaccg cgctggtttc cgccggctcg tcgtcgttga ggttcaggat gcgcgtgccc
      901 ggcggccgct gctgccgcat cgccgccagc accgcgcgcg tgatgtcgtc gcgatggatg
      961 cggccgaagg cgtggcccgg cttggcgata cgccgcgccc ggccggcgcg cagatcatcg
     1021 aaggcggagc ggccagggcc atagatcccg gccaggcgga agacgtccac cgcgcgccgg
     1081 ccggcgaaac gcgaccacgc cagttccgca tccagccgcc gctgcccacg ttcctggctc
     1141 ggctgcggcg gcgtgtcctc gtccacccag ccgccggcgc gatcgccata cacgacggtg
     1201 ctggacaggt agccgatcca acgcagcgcc ggtgccgctt cgatctgggc ggcgtaacgt
     1261 gccagcaccg gatcgccgga ggcgtccggc ggcgcggtgg acagcaggtg tgtcgcctgg
     1321 gcgacggcct cgccggcctg ctggaacgcg atgccgccgt cggcggcgga ctcgcgcgac
     1381 gtaccgcaga ccgcaatgcc attggcgcgg gcaagcgccg cgacggcgcg gcctgtgtac
     1441 cccagtccga aaatcagcag catcattcag tccgatccag gcaatcggct tgcaagggtc
     1501 atctcccaac gccatgccaa cctgttccac ggcagcacgc gcgctacgcc aaggcaatcg
     1561 ccgcacggct ggcctgcgcc gagcgaagct gcctaagctg gggacaggcg aaggttggct
     1621 gcaaggccgc gggaggaacc ccatgcaaag ctggcgcatc ggcgacgtga cggtaagcaa
     1681 actggtggaa ctggaaatga ccggcggcag ccgcttcctg ctgccacagg ccacgccgga
     1741 agagatcaag ccgatcggct ggctgcagcc gcacttcgcc gacgaaaacg gcaagctgcg
     1801 catgagcatc cacagcttca tcgtgcagac tcccacgcgg cggatcatcg tcgatacctg
     1861 cctgggcaat gacaagcagc agcgccgcat cccgcactgg aacgaccggc aaggtccgtt
     1921 cctgaccgac ctcgccgcgg ccggttttcc gcgcgagtcg atcgacactg tgctgtgcac
     1981 ccatctgcat gtcgatcacg tcggctggaa caccatgaag gtggacggga aatgggtgcc
     2041 cactttcccg caggcccgct acctgatggg ccgcagcgaa tacgagcact ggcgcgacaa
     2101 caccgcgcgc gaggacatgc ggcacatcct ggcggattcg gtgacgccga tcctggacgc
     2161 cggcctggcg gaactggtgg aaaccgacca ccagctcacg gacgagatca gcctggtacc
     2221 gacgctcggc cacacgcccg gtcatgtcag cgtgcgcatc gtgtcacgcg gcgaggaagc
     2281 gctgatcacc ggcgacttca tgcaccaccc ctgccagatc gcccatccgg agtggagcac
     2341 gctggccgac agcgatccgg aacagggtat ccgcacgcgc tggcggatgt tcgagcaact
     2401 ggcgggcaag ccggtgctgg tgatcggcac gcatttcgcc ggcgtgacgg cggggcggct
     2461 ggtgcgcgac ggcgcgacgt tccggctggc ggtgtgaaaa aggaagaatg gaaccgcaga
     2521 tgaacgcgga tgtcgcgagg cggccgatct gccgagcgaa ctcccgccga aggcgatgat
     2581 tatgctgtca tcgactgaag ctaagcaggt gactccgtgg cggaccgctc aagcgcatcc
     2641 gtgttcatcg gcgttcatct gcggttccaa ggcttgactg aatctgaagc cgaacccgga
     2701 cccgggttca caccgcgccg cccgcccaga cgacccggcc gatcacgcgc agttc
//
LOCUS       DASSZH010000652         2344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1055728, whole genome shotgun sequence.
ACCESSION   DASSZH010000652 DASSZH010000000
VERSION     DASSZH010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055728"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1569)
                     /locus_tag="VFN42_14130"
     CDS             complement(<1..1569)
                     /locus_tag="VFN42_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET8997802.1"
                     /translation="MTAIEPIAYTDAQLRRILATVRTIAMVGASTNWNRPSYFVMKYL
                     QGKGYRVIPVNPGAAGQELQGEHVYASLRDIPNKIDMVDIFRPSAAVPPIVEDAIAIG
                     AKIVWMQLGIRNDEAAARAEAAGLEVVMNRCPKIEFGRLGGELSWSGVNSGIIRNRAP
                     EAPTERHAKPRPLPQHNITYGFETRAIHAGAAPDPTTGARNTPIFQTTAYVFDDVDHA
                     ASLFNLHNFGYIYTRLTNPTVAVLEERVANLEGGRAAVAAASGHAAQFLVFATMLEPG
                     DEFLASRNLYGGSLTQFGLSFKKLGWTCHFVDPTDPENFRRAMTPKCKAIFVESLANP
                     GGVIVDLEPVAKIAHESGIPLIVDNTLATPYLCRPFEYGADLVTHSTTKFLDGHGNSL
                     GGIVVESGKFDWGQGGKFPALTEPEPAYHGLRFYENFGDFAFTTKARAVALRDFGPTL
                     APMNAFLTITGIETLHVRMERHCANAQAVAEFLAQDPRVAWVSYAGLESSPFRARARK
                     YLPKGAGAVFTFGVK"
     gene            1715..>2344
                     /locus_tag="VFN42_14135"
     CDS             1715..>2344
                     /locus_tag="VFN42_14135"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HET8997803.1"
                     /translation="MSDAATVTALSRVLSDYVARTAPSLVSIRAGRSGTSGFVWRPGL
                     IVTAEEPLPEEDSFAVVTAAGETLTAQRAGRDPSTDIALLRVNRTDLPTVSLSSVPVT
                     AGTLALAIGAEEGAPTAAFGVVSRSTGAWRSLRGGEIDARVELDVRLRRSAEGGLVLD
                     AAGQAIGMAVLGPRRRTLVVPSATIERVANTLATHGRIARGYLGLGMQPV"
BASE COUNT          375 a          768 c          815 g          386 t
ORIGIN      
        1 cttcaccccg aaggtgaaca ccgcgcccgc gcctttcggc aggtatttgc gcgcccgtgc
       61 acgaaacggg ctggactcca gtcccgcata ggacacccat gccacgcgcg ggtcctgcgc
      121 caggaactcc gccaccgcct gcgcattggc gcaatgccgc tccatgcgca catgcagcgt
      181 ctcgatgccg gtgatggtca ggaacgcgtt catcggtgcc aaggtgggcc cgaagtcgcg
      241 cagcgccact gcacgcgcct tggtggtgaa agcgaagtcg ccgaaattct cgtagaagcg
      301 cagcccgtga taggcgggct ccggctcggt cagcgcgggg aactttcccc cctgccccca
      361 atcgaacttt ccagactcca ccacgatgcc gccgagcgaa ttgccgtggc catcgaggaa
      421 tttcgtggtg gaatgcgtca ccaggtccgc gccgtactcg aacggccggc acaggtacgg
      481 cgtggccagc gtgttatcga cgatcagcgg aatgccggac tcatgcgcga tcttcgccac
      541 cggttccaga tcgacgatga cgccgcccgg attggccagg ctctccacga agatcgcctt
      601 gcatttcggc gtcatcgcgc ggcggaagtt ctccggatcg gtcggatcga caaaatggca
      661 ggtccagccg agctttttga acgacaggcc aaactgggtc agcgaaccgc cgtacaggtt
      721 gcgtgacgcc aggaattcgt cgccaggctc cagcatggtg gcgaacacca ggaactgcgc
      781 ggcatgtccg ctcgccgccg ccaccgcggc gcggccgcct tccaagttgg cgacgcgttc
      841 ctccagcacc gcgaccgtgg gattggtcag ccgcgtgtag atgtagccga aattgtgcag
      901 gttgaacagc gacgcggcat gatcgacgtc gtcgaagacg taagcggtgg tctggaagat
      961 cggcgtgttg cgtgcgcccg tggtgggatc gggcgcggcg ccggcgtgga tggcgcgcgt
     1021 ctcgaacccg taggtgatgt tgtgctgcgg cagcgggcgc ggcttggcgt ggcgttcggt
     1081 gggcgcctcc ggtgcgcggt tgcggatgat gccggagttc accccgctcc acgacagctc
     1141 gccgccgagc cggccgaatt cgatcttcgg gcagcgattc atgacgactt ccaggccggc
     1201 ggcttcggcg cgggcagcgg cttcatcgtt gcggataccc aactgcatcc acacgatttt
     1261 cgcaccgatg gcgatggcat cctcgacgat cggcggcacg gctgccgacg ggcggaagat
     1321 atcgaccatg tcgatcttgt tgggaatgtc gcgcaggctg gcatagacat gctcaccctg
     1381 cagctcctgg ccggcggcgc cggggttcac cggaatgacg cgatagcctt tgccctgcag
     1441 atacttcatg acgaagtagc tgggccggtt ccagttggtg gaggcgccga ccatggcgat
     1501 ggtgcgcacc gtggccagga tacggcgcag ctgcgcatcg gtgtaggcga tcggttcgat
     1561 ggcggtcatc gctggctcct ccgagcagat tgcggtcgcc cgctggcatg ccgcgcctgc
     1621 cgcgggctga caagggcggt cctgcccaac cgggcagtag gcatgccggg gtgcccgaca
     1681 cacattgcgg accaacgaac cgatggagag actcatgtcc gatgccgcca ccgtcaccgc
     1741 cctgtcgcgc gtgctcagcg actatgtcgc gcggacggcg cccagcctgg tgtcgatccg
     1801 cgccggccgc agcggcacca gcggcttcgt ctggcgtccc ggcctgatcg tcaccgccga
     1861 ggaaccgctg ccggaggagg acagcttcgc ggtggtcacc gccgccggcg agaccctcac
     1921 cgcccagcgc gccgggcgcg accccagcac ggacatcgcc ctgctgcggg tgaatcgcac
     1981 cgacctgccg acagtgtcgc tatccagcgt gcccgtgacc gcaggcacgc tggcacttgc
     2041 catcggagca gaggaaggcg cgcccaccgc ggcctttggt gtcgtgtcgc gcagcaccgg
     2101 tgcgtggcgg tcgctgcgcg gcggcgagat cgacgcgcgg gttgaactgg atgttcggct
     2161 gcggcgcagc gcggaaggcg gactggtgct ggacgcggcg ggacaggcga tcggcatggc
     2221 ggtgctgggg ccgcggcgcc gcacgctggt ggtgccctcc gccacgatcg agcgcgtggc
     2281 caacacgctg gcgacgcatg ggcggatcgc acgcggctat ctgggattgg gcatgcagcc
     2341 ggtg
//
LOCUS       DASSZH010000653         4519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1669258, whole genome shotgun sequence.
ACCESSION   DASSZH010000653 DASSZH010000000
VERSION     DASSZH010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669258"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1461)
                     /locus_tag="VFN42_14140"
     CDS             complement(<1..1461)
                     /locus_tag="VFN42_14140"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014106703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET8997804.1"
                     /translation="MLDKTFSPQEIEPRLYQGWEESGAFACDPTSKAQPFCIMIPPPN
                     VTGSLHMGHALTFTVQDTLIRWRRMQGRDVLWQPGTDHAGIATQMVVERLLAEQGGPD
                     RRTMGREAFIERVWQWKAESGGTITKQLRRLGASLDWPRERFTMDEGLSRAVREVFVT
                     LYRQGLIYRDRRLVNWDPKLHTAISDLEVENREIKGHLWHISYPIEGDPGRCIIVATT
                     RPETMLGDTAVAVHPADERYRDLVGKHAILPLVGRRLPIVADEYSDPEKGTGAVKITP
                     AHDFNDFEVGRRHDLPMPSVLDRAARVTLAEIADALTLVEGVADPGFVRTLDGDSRES
                     ARKKIVAELERLNLLNKVEPHTHQVPHGDRGGVPIEPLLTTQWYANAEELAKPAIAAV
                     ETGKTAFVPKQWENTFFAWMRDIQPWCISRQLWWGHRIPAWYGPDGAVFVAHTEEEAR
                     AEARAKYGRDEELVQDEDVLDTWFSSGLWPFSTLGWP"
     gene            complement(1539..2075)
                     /locus_tag="VFN42_14145"
     CDS             complement(1539..2075)
                     /locus_tag="VFN42_14145"
                     /inference="COORDINATES: protein motif:HMM:NF022155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2497 domain-containing protein"
                     /protein_id="HET8997805.1"
                     /translation="MSGSQTPPGGGTGRDQPGADPSMEDILASIRRILSEDVPQPATL
                     ETSPPPGDSPSDVLVLDQSMMVPEPVPAEPPRAPEPVAEARPAAPERRPESRRDGDDL
                     VAPEAAAATALAVNSLVRRLSAERGTQVRSGGPTIEDMVREELRPMLKQWLDTYLPPM
                     VERLVKAEIERVVGRVVP"
     gene            complement(2092..3567)
                     /locus_tag="VFN42_14150"
     CDS             complement(2092..3567)
                     /locus_tag="VFN42_14150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HET8997806.1"
                     /translation="MVPRHRLLFSLVLSTALAGSALAQPSRTKHEPHKPPPPGSPAQY
                     NTAAAPHTLAGALASTYANEPALLAERAKLRATDENVPAALSGWRPTVVLAGTAGYGN
                     GVTRSYSPFLGHYSLSSERDLATGQATLTQPIYNGGRTQANVNRSKNQVMAERAALMV
                     QEQTSFQNTVNAYVGVIQAQQIVQLDKNNVEVLSRQLQATRDRFRVGEITRTDVAQAE
                     AALSGAESTLESDQGSLQSARATFVQTVGYVPPADLVEPQPLQLPVRSEQQASAIAAA
                     NNPNVVEAKFNNAAAKDAVDVAFSQLMPQVSLQAQTFAQENSGGRSSQATGYQVVAQL
                     SVPLYQGGSEYAAVRQAKQTAQQTDRLITYQERSAVQAAVQAWDTYVAAKAAAVSSRA
                     AIRANEIALQGVEREAIVGSRTTLDVLNAQQALLQSRTTLVANLAALVKASYSVAGAI
                     GRLTARDLRLNVPLYDDTAYYKAVKDRWFGLGDYATGQPNR"
     gene            complement(3721..>4519)
                     /locus_tag="VFN42_14155"
     CDS             complement(3721..>4519)
                     /locus_tag="VFN42_14155"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8997807.1"
                     /translation="PYSVHFSYDTYALAHGTGGALTRAGGKTWFFVTADYAFGYALER
                     DTIAAVEATGGKVLGKVRAPLGTPDFSSFLIQARASGAEVIGFANAGTDLQNCIKQSA
                     EFGLADGKTKLATLLMQISDVNSIGQKACAGLIYTDSFYWDMTPKTRAWSKRWGAKMG
                     GMVPGLLHAGSYAAAYHWLKAVKAAGTTEGDAVMAKMKATPVNDFYNTNVKVREDGRV
                     MHVMYLWQVKPVSESKYKYDFCKHLATIQPSEAWRPLDQGGCPLIKA"
BASE COUNT          629 a         1560 c         1548 g          782 t
ORIGIN      
        1 cggccagccc agggtggaga agggccacag gccactgctg aaccaggtgt ccagcacgtc
       61 ctcgtcctgc accagctcct cgtcgcggcc gtatttcgcg cgggcctcgg cgcgcgcttc
      121 ctcttcggta tgcgccacga acacggcgcc gtccggaccg taccaggcgg ggatgcggtg
      181 accccaccaa agctggcggg agatgcacca gggctggatg tcgcgcatcc aggcgaagaa
      241 ggtgttctcc cactgcttcg gcacgaacgc cgtcttgccg gtctccaccg ccgcgatcgc
      301 cggcttcgcc agttcctcgg cattggcgta ccactgggtg gtcagcagcg gctcgatcgg
      361 cacgccgccg cggtcgccgt gcggcacctg gtgggtgtgc ggctccacct tgttcaggag
      421 gttcagccgc tcgagttcgg cgacgatctt cttgcgcgct gactcgcggc tgtcgccgtc
      481 cagcgtgcgg acgaagcccg gatcggccac gccttccacc agcgtcagcg cgtcggcgat
      541 ctccgccagg gtcacgcggg cggcgcggtc cagcaccgag ggcatcggca ggtcgtgccg
      601 gcggcccacc tcgaagtcgt tgaagtcgtg cgccggagtg atcttcaccg cgccggtgcc
      661 cttttccggg tcggagtatt cgtccgccac gatcggcagg cgccgcccga ccagcggcag
      721 gatggcgtgc ttgcccacca ggtcgcggta gcgctcatcc gccggatgca ccgccaccgc
      781 ggtgtcgccg agcatggttt cggggcgggt ggtggccaca atgatgcagc ggccagggtc
      841 gccctcgatc gggtagctta tgtgccagag atggcctttg atctcccggt tctccacctc
      901 caggtcggag atcgcggtgt gcagcttggg gtcccagttc accagccggc ggtcgcggta
      961 gatcagcccc tggcggtaca gcgtgacgaa cacctcccgc acggcgcggg acaggccctc
     1021 gtccatggtg aagcgctcac gcggccagtc caggctggcg cccagccggc ggagctgctt
     1081 cgtgatggtg ccgccggatt ccgccttcca ctgccagacg cgctcaatga acgcctcccg
     1141 ccccatcgtc cggcgatccg ggccgccctg ctctgccagg aggcgttcca ccaccatctg
     1201 ggtggcgatg ccggcatggt cggtgccggg ctgccacagc acgtcgcggc cctgcatgcg
     1261 ccgccagcgg atcagcgtgt cctgcaccgt gaaggtcagg gcgtggccca tatgcaggct
     1321 gccggtgacg ttcggcggcg ggatcatgat gcagaagggc tgcgccttcg acgtggggtc
     1381 gcaagcgaac gcgccgctct cctcccaccc ctggtagagg cgaggctcga tctcctgcgg
     1441 cgaaaaggtc ttgtccagca tcatggtctc ctgcggcccc tcgtttggcc cctctgaggg
     1501 accgtcaggt gccgcaggag gccgtcaatg gcaatgcttc agggaacgac gcggccgacg
     1561 acgcgctcga tctcggcctt caccagccgc tcgaccatcg gcggcaggta ggtgtccagc
     1621 cactgcttca gcatcggccg caattcttcc cgcaccatgt cctctatggt cgggccgccg
     1681 ctgcgcacct gggtgccgcg ctccgccgac agccggcgga ccaggctgtt caccgccagg
     1741 gcggtggcgg cggcggcttc cggggcgacc aggtcgtcgc cgtcccggcg gctctccggc
     1801 cggcgttctg gcgcggccgg gcgggcttcg gccaccggct cgggtgcgcg cggcggttcg
     1861 gccgggaccg gctccggcac catcatcgat tgatccagca ccagcacgtc gctgggcgag
     1921 tcaccgggtg gcggggaggt ctccagcgtg gccggctgcg gcacgtcctc gctcaggatg
     1981 cgccggatcg aggccaggat gtcttccatc gacggatcgg cgcccggctg gtcccggccc
     2041 gtcccgccgc ccggcggcgt ttgcgatccg ctcatcactc agctccaaag cctatcgatt
     2101 gggttggccg gtcgcatagt cgcccaagcc gaaccagcga tccttcaccg ccttgtagta
     2161 ggcggtatcg tcgtacagcg gcacgttcag ccgcaggtcg cgcgcggtca gccggccgat
     2221 ggcgccggcc accgagtagg atgccttcac cagcgccgcc aggtttgcca ccagggtggt
     2281 gcgcgactgc aacagtgcct gctgcgcgtt cagcacgtcc agcgtggtgc ggctgccgac
     2341 gatggcttcc cgctccaccc cttgcagggc gatctcgttg gcgcggatcg cggcgcgcga
     2401 gctgacggcg gccgccttgg cggccacata ggtgtcccag gcctgcaccg cggcctgcac
     2461 cgcggagcgc tcctgatagg tgatcagccg gtcggtctgc tgcgcggtct gcttcgcctg
     2521 gcggaccgcg gcgtattccg agccgccctg gtacaagggc accgagagct gcgccaccac
     2581 ctggtagccg gtcgcctgtg atgagcggcc gccggaattc tcctgcgcga aggtctgcgc
     2641 ctgcaggctg acctgcggca tcagttgcga gaaggccacg tccaccgcgt ccttggcggc
     2701 ggcgttgttg aacttggcct ccaccacgtt cgggttgttc gccgcggcaa tcgccgaggc
     2761 ctgctgctcg ctgcgcaccg gcaattgcag cggctgcggc tccaccaggt cggccggcgg
     2821 aacgtagccg acggtctgca cgaaggtggc gcgcgcgctc tgcaggctgc cctgatcgct
     2881 ttccagggtg gattccgccc ccgacaacgc cgcctcggcc tgcgccacgt cggtgcgggt
     2941 gatttcgccg acccggaacc ggtcgcgggt ggcctgcaac tgccggctca gcacctcgac
     3001 gttgttcttg tccagttgga cgatctgctg cgcctggatg acaccgacat aggcgttgac
     3061 ggtgttctgg aagctggtct gctcctgcac catcagcgcg gcgcgctccg ccatcacctg
     3121 gttcttggac cggttgacgt tcgcctgggt gcgcccgcca ttgtagatgg gctgggtcag
     3181 ggttgcctgg ccggtggcca ggtcgcgctc gctcgacagc gagtagtggc cgaggaacgg
     3241 cgagtagctg cgggtgacgc cgttaccgta gccggccgtc cccgccagca ccaccgtagg
     3301 gcgccagccg gacagcgccg ccggcacatt ctcgtccgtc gcgcgcagct tggcccgctc
     3361 ggccagcaag gccggttcgt tggcataggt ggaggccagg gcgccggcca gcgtgtgcgg
     3421 tgcggcggcg gtgttgtatt gcgcgggact gccgggcggc ggcggtttgt gcggctcatg
     3481 cttggtccgt gacggctggg cgagcgcgga gccggccaag gccgtggaca agacaaggct
     3541 gaacagcaag cgatgacgcg gtaccatgcg aaccctcatg ccttcggcct tccgatgtgc
     3601 cacaggccag cccgtctcgc cagagcggag cgcggtaagc cgatcaaggc tgggcgaccc
     3661 gtaacgggcc ggaacggcac gccacgggtc gcatcctgcg ctggtttggt taaccagccc
     3721 ctaagccttg atcagcgggc agcctccctg gtcgagcgga cgccaggcct cggacggctg
     3781 gatcgtcgcc agatgcttgc agaaatcata cttgtacttg gattccgaca ccggcttgac
     3841 ctgccacaga tacatgacgt gcatgacgcg gccgtcctca cgcaccttca cgttggtgtt
     3901 gtagaagtcg ttcaccggcg tcgccttcat cttggccatc accgcatcgc cttcggtggt
     3961 gccggcggcc ttcaccgcct tcagccagtg ataggcggcg gcgtagctgc cggcatgcag
     4021 caggccgggc accatgccgc ccatcttcgc gccccagcgc ttggaccagg cgcgggtctt
     4081 cggcgtcatg tcccagtaaa acgagtcggt atagatcagc ccggcgcagg ccttctgccc
     4141 gatcgagttc acgtcgctga tctgcatcag caatgtggcc agtttggtct tgccgtcggc
     4201 caggccgaat tccgccgact gcttgatgca gttctgcaga tccgtgccgg cattggcgaa
     4261 gccgatcacc tcggcgccgg aagcgcgcgc ctggatcagg aagctggaga aatccggcgt
     4321 gccgagcgga gcgcgcacct tgcccagcac cttgccgccg gtggcttcga cggccgcgat
     4381 ggtgtcgcgc tccagcgcat agccaaaggc gtagtccgcg gtgacgaaga accaggtctt
     4441 gccgccggcc ctggtcagcg cgccgccggt gccgtgcgcc agcgcgtagg tgtcgtagga
     4501 gaagtgcacg ctgtacggc
//
LOCUS       DASSZH010000654         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_67209, whole genome shotgun sequence.
ACCESSION   DASSZH010000654 DASSZH010000000
VERSION     DASSZH010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67209"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VFN42_14160"
     CDS             <1..453
                     /locus_tag="VFN42_14160"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Acylates the intermediate (KDO)2-lipid IVA to form
                     (KDO)2-(lauroyl)-lipid IVA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HET8997808.1"
                     /translation="SAVVYRMPNNKAVAKEIARIRAPLMGRLIRTRAQAALEMAAALQ
                     AGEHLGMLIDQHFSRGVDVTFFGRRCKANPSIARLARQFDCPVVGVRVVRLPDRRFRI
                     EGEGPFDLPRDAAGQIDVRAATQMLTTVIEGWIRQHPEQWLWFHRRWR"
     gene            551..1093
                     /locus_tag="VFN42_14165"
     CDS             551..1093
                     /locus_tag="VFN42_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997809.1"
                     /translation="MRSVVLSVPVWLLLGAAALAQGTANAPPPLPPSDQAASPLPPNL
                     APPAAMPAPAQQPATLGGQEPVSTGAANLNQQDQPYRKVAPALPTPELGPNATPVDYL
                     HAAEAALAGGRTGEAQQSLEMAQTRLLDRSVPYGQVNTPIKSPAIDTISQALQALGAG
                     DRAQTMQLIQAAIHQAEAAR"
     gene            1199..>2018
                     /locus_tag="VFN42_14170"
     CDS             1199..>2018
                     /locus_tag="VFN42_14170"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET8997810.1"
                     /translation="MAAVEQFGGEQTLTFRAPEGWGKREALPESAAHLLLLMREGEST
                     PQRIPLSTLPAIIGRSPPADILLEGGTVSRRHCRLALRHDQLILTDLGSTNGTFVDGA
                     RLEGEVALQDGALLAIGAHRLRYHRRTQGETAEAEAMDRELRDAAAYVASILPPPIDS
                     GPIQAEWFYQPSARLGGDAFGYQWLDEHTFAAFVLDVAGHGAAAALHAVSVVNVLRQR
                     LLPGVDLRDPAAVIRNLNRMFPIERHNSLFLTIWYGVYDTSNRMLAYATGGHHAA"
BASE COUNT          320 a          746 c          662 g          290 t
ORIGIN      
        1 tcggccgtgg tctatcgcat gccgaacaac aaggcggtgg cgaaggagat cgcccgcatc
       61 cgcgcgccgc tgatgggccg gctgatccgc acccgcgccc aggcggcgct ggagatggcc
      121 gccgcgctgc aagccggtga gcacctgggc atgctgatcg accagcattt ctcccgcggc
      181 gtggacgtca ccttcttcgg ccggcgctgc aaggcgaatc cgtcgatcgc gcgcctcgcc
      241 cgccagttcg attgcccggt ggtcggcgtg cgcgtggtgc gcctgcccga ccgccgcttc
      301 cgcatcgaag gcgagggccc gttcgacctg ccgcgggatg ccgccggcca gatcgatgtg
      361 cgggccgcca cccagatgct gaccaccgtc atcgaaggct ggatccgcca gcaccccgag
      421 cagtggctct ggttccaccg ccgctggcgc tgaccgcgct cacgtttttg ctaccacaac
      481 gaacccgcgt gcgattgacc gggctgcgct gcaccccaga tgccagcgca accgcaacag
      541 gagaagcttc atgcgcagtg tggtcctgtc cgtccctgtc tggctgctgc tgggtgccgc
      601 cgccctggcg caggggaccg cgaatgcgcc acctcccttg ccgccgtcgg atcaggcagc
      661 ctccccgctg ccgccaaacc tcgcgccgcc ggccgcaatg ccggcacccg cgcaacagcc
      721 ggccaccctg ggtgggcagg agccggtttc gaccggtgcc gccaacctga accagcagga
      781 ccagccatat cggaaggtgg cgccggcgct gcccacgccg gagctgggcc cgaatgccac
      841 tccggttgac tacctgcacg cggccgaggc ggcgctggcc ggcgggcgga ccggcgaggc
      901 acagcaatcc ctggaaatgg cgcagacccg gttgctggac cgctcggtgc cgtacggcca
      961 ggtgaatacc ccgatcaagt caccggcgat cgacaccatc agccaggccc tgcaggcgct
     1021 gggggccggc gaccgggcgc agaccatgca actgatccag gccgccatcc atcaggcaga
     1081 agcggcgcgt tagatcggcc gcccgggccg accaggcgag ctgccttcac acaaccattg
     1141 cgtttcccgc ccaaaacccc aacactgccc cagacctgag gctttggcgg aaacacgcat
     1201 ggcagcggtc gagcaattcg gcggcgagca gaccctgacc ttccgcgctc cggagggatg
     1261 gggaaaacgg gaagccttgc ccgaatccgc cgcgcatctg ctgctgctga tgcgggaggg
     1321 cgagagcacg ccccagcgca tccccttgag caccctgccg gccatcatcg gccgcagccc
     1381 gccggcggat atcctgctgg aaggcggcac cgtgtcccgc cggcactgcc gcctggcctt
     1441 gcggcatgat cagctgatac tgaccgacct gggctcgacc aacggcacct tcgtcgatgg
     1501 ggcacggctc gaaggtgagg tcgccctcca ggacggcgcg ctgctggcca tcggtgccca
     1561 ccgcctgcgc tatcaccgcc gcacccaggg ggagaccgcg gaagccgagg cgatggaccg
     1621 cgaattgcgc gacgccgcgg cctatgtggc ctccatcctg cccccgccga tcgacagcgg
     1681 cccgatccag gccgaatggt tctaccagcc cagcgcccgg ctcggcggcg acgcgttcgg
     1741 ctatcagtgg cttgacgagc ataccttcgc cgccttcgtg ctggacgtcg ccggccacgg
     1801 ggcggcggcg gcactgcatg cggtgtcggt ggtgaacgtg ttgcggcagc ggctgctgcc
     1861 cggcgtcgat ctccgcgatc cggcggcggt gatccgcaac ctcaaccgca tgttccccat
     1921 cgaacggcac aactcgctgt tcctgacgat ctggtacggc gtgtacgaca cgagcaaccg
     1981 catgctggcc tatgccaccg gcggacacca cgccgcct
//
LOCUS       DASSZH010000655         5224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1055786, whole genome shotgun sequence.
ACCESSION   DASSZH010000655 DASSZH010000000
VERSION     DASSZH010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055786"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1170)
                     /locus_tag="VFN42_14175"
     CDS             complement(<1..1170)
                     /locus_tag="VFN42_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997811.1"
                     /translation="MRPLAALALGALLFGAWAGLSERVVTHAAGQARQQIVLAAQGIA
                     GRIDAGFGRFADQVLAATPADFAPGDRVGETARLLRLQNLLPHAAATFMLAPDGHLLA
                     ASAPFLPADAKVGGTDWFGTALAAPDNQIAVQALAQPWFAIQSGIVLTRTVAGPAGSP
                     VGLLGVVLPAAELTQLSTPAWLQPSMSVLLLGGTSGTVLTGAPPEPAPQPWLSQAMQA
                     VLAWSGTPADWSASVPLHAFNATVIATMAPAAALPAPLGRAILAAGAATLLAWLLAVF
                     AIFRRPRLVGVPTQAGFGTDWQCALDSEGRVQALHGLVPGALAAAHNLPLATALGLPP
                     DSVDAEQITDALRQRAATTLQVELAGRTYQVTLAPAPPDDFVCSGRDVSEQVAAIA"
     gene            complement(1310..1849)
                     /locus_tag="VFN42_14180"
     CDS             complement(1310..1849)
                     /locus_tag="VFN42_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997812.1"
                     /translation="MHPQARSRPRTPNGPIAVLLGSVLLASCAHVAPPPPPPQPPPAR
                     PVAERPRLHPVWSFQTGPNACLAVATAGHTRLLVAVRRKQPIRLSVTTPYDTAGQAVA
                     HFHGPAGAWIASGWHAGGHEIVFTLGRDMDSLSRVLMLLSGGVMDLDAPEKNLPIFIL
                     PAAGPHGQQWFGCARKLVI"
     gene            complement(1887..2651)
                     /locus_tag="VFN42_14185"
     CDS             complement(1887..2651)
                     /locus_tag="VFN42_14185"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: protein motif:HMM:NF003986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HET8997813.1"
                     /translation="MNPSRHSRPPLGRLALLALLLSFGLAGRAAADPSALWHIVHGKC
                     VPDLVQKHNPAPCAAVNLEGGYAVLKDRRGPYQFLLIPTARVSGIDDRSILAPGAPNY
                     WQAAWQARHFLEQRVGRAVPRDVLSLAINSALGRSQNQLHIHVDCIRPDVRAALRQHL
                     EAVGPHWAPFPVPLAGQRYRAMRIVQADLKGINPFDLLARSIPASEMRRHTLVVAAVM
                     FGEHPGFVLLDDRANPLHGNFGHGESLQDHTCALLR"
     gene            2762..3397
                     /locus_tag="VFN42_14190"
     CDS             2762..3397
                     /locus_tag="VFN42_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HET8997814.1"
                     /translation="MSLFSLHGLAHLTQSYGPWLLGVVVMLEAMGLPLPAESLLIATA
                     AFAGSSHHLAVAWIVIPAAAGAIVGDNLGYLIGRSLGWRVLRRWGRHIGLTDDRLTLG
                     AYLFRCHGGKVVFFGRFVAVLRTLAALLAGANRMNWWWFLPCNAAGGILWSCVYGLGA
                     YWLGKEVERVARPAGFAIGLLAAAVIVTVFLLIRRHEQELTARAKREMRGA"
     gene            3472..4776
                     /gene="glyA"
                     /locus_tag="VFN42_14195"
     CDS             3472..4776
                     /gene="glyA"
                     /locus_tag="VFN42_14195"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET8997815.1"
                     /translation="MNEQSARASQHRFFTARVADTDPELAAALDGELARQQDGIELIA
                     SENIVSAAVLEAQGSTLTNKYAEGYPGRRYYGGCVQVDIAERLEIARAQQLFGCGFAN
                     VQPHSGAQANQSVFLALLQPGDTILGMSLAAGGHLTHGAGPNLSGKWFRAVQYGVRRD
                     DGLLDYDELERLARTEKPKLIIAGGSAYPRFIDFARIRRVADEVGAYFMVDMAHFAGL
                     VAAELFPSPLPHAHVVTTTTHKTLRGPRGGMVLSNDPEIGKKINSAVFPGLQGGPLMH
                     VIAAKAVAFGEALRPEFRLYQKSVVENARVLAETLQQAGLAIVTGGTDCHLMLVDLRP
                     KRVTGKAAEASLERAHITANKNAIPFDPEKPAITSGIRLGSPAATTRGFGAAEFREVG
                     NMINEVLEGLAGSNDGSNDAAERAVGERVRALCARFPLYNAG"
     gene            4783..>5224
                     /gene="nrdR"
                     /locus_tag="VFN42_14200"
     CDS             4783..>5224
                     /gene="nrdR"
                     /locus_tag="VFN42_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HET8997816.1"
                     /translation="MRCPFCGHEDTQVKDSRPSEDGSAIRRRRFCGNCGQRFTTVERT
                     QLRELTVVKQDNRRVPFDRDKLARSIRIALRKRPIQEERIERIVNSIVRKLEASGDAD
                     IPSKQIGELVMDTLKELDPVAYVRFASVYRNFREAKDFEDFVGSL"
BASE COUNT          835 a         1792 c         1846 g          751 t
ORIGIN      
        1 ggcgatagcg gcaacctgct cgctgacgtc gcggccggaa cagacgaagt cgtccggcgg
       61 ggccggtgcg agcgtgacct ggtaggtgcg tcccgccagc tccacctgca gtgtcgtggc
      121 ggcgcgctgg cgcagcgcat cggtgatctg ctcggcatcc accgagtcgg gcggcaggcc
      181 gagcgcggtc gccagcggca gattgtgggc cgcggccagg gcgcccggca ccagcccatg
      241 cagggcctgc acgcgccctt cggaatccag cgcgcactgc caatcggtgc cgaaacccgc
      301 ctgggtcggc acgccgacca gcctgggccg gcggaagatc gcgaacaccg ccagcagcca
      361 ggccagcagc gtggctgcac cggcggcgag gatcgcgcgg cccagcggcg ccggcagcgc
      421 ggcggcgggc gccatggtgg cgatcaccgt ggcattgaac gcatgcagcg gaaccgaggc
      481 gctccagtcg gccggcgtgc cggaccaggc cagcacggcc tgcatcgctt ggctcagcca
      541 gggctgcggc gccggctccg gcggcgctcc ggtcagcacc gtcccgctgg tgccgccgag
      601 cagcagaacc gacatgctgg gctgcagcca ggcgggggtc gaaagttgtg tcagctcggc
      661 tgccggcaga accacgccca acaggccgac cggcgatccg gcggggccgg ccaccgtgcg
      721 ggtcagcacg atgccggact ggatggcaaa ccagggctgc gcgagcgcct gcacggcgat
      781 ctggttgtcc ggagcggcca gcgcggtgcc gaaccagtcc gtcccgccca ccttggcatc
      841 ggcgggcagg aagggcgccg atgcggccag cagatgtccg tccggtgcca gcatgaaggt
      901 ggcggccgca tgcggcagca ggttctgcag gcgcagcagc cgagccgtct cgcccacccg
      961 gtcgcccggt gcgaaatccg ccggcgtggc agccagcacc tgatcggcga atcggccgaa
     1021 cccggcatcg atccggccgg cgatcccctg cgccgcgaga acgatctgct gccgggcctg
     1081 cccggcggca tgcgtcacga cccgttcgct caatccggcc caggcgccga acaacagggc
     1141 acccagggcg agggcggcga gcggcctcac gaccattcct gtcgccgatg cgcgaacggc
     1201 gcctgccgaa ttcactgcca tcgtacctcc gtggaacagg ctcaccctag ccgatcgctg
     1261 gttgcagcgg cttttattaa atccttatcc acagccgaaa atttcacaat tagataacga
     1321 gcttccgggc gcagccaaac cactgctggc cgtgcgggcc ggccgctggg agtatgaaaa
     1381 tgggcagatt cttctccggc gcatccaggt ccatcacccc gccgctgagc aacatgagga
     1441 cgcggctgag tgagtccata tcgcgtccga gcgtgaacac aatttcgtgc ccgccggcat
     1501 gccagccgga agcgatccag gcgccggcgg ggccgtggaa atgcgcgacc gcctggccgg
     1561 cggtgtcata gggcgtggtg acgctcaacc ggatgggctg cttgcgccgc accgcgacca
     1621 gcagccgcgt atgccctgcc gtggcgaccg cgaggcaggc attggggccg gtctggaacg
     1681 accataccgg gtgcagccgc ggacgttcgg cgaccggccg ggccggcggc ggctggggcg
     1741 gcggtggtgg cggcgcgaca tgcgcacagg aggcgagcag gaccgatccc agcaggacgg
     1801 cgatcggtcc gttcggcgtc cggggccggg agcgggcttg cgggtgcatt ccacctgcat
     1861 actagcacag gctcggcaac ggagcgctat cgcagtaatg cgcaggtgtg atcctgcagg
     1921 ctctccccgt ggccgaaatt gccatggagc gggttcgcgc ggtcgtccag cagaacgaaa
     1981 ccgggatgct ccccgaacat cacggcggcg accaccaggg tgtgccggcg catttcgctg
     2041 gccgggatcg agcgggcgag cagatcgaac ggattgatgc ccttcaggtc ggcctgcaca
     2101 atccgcatcg cccggtagcg ctggccggcg agcgggacgg ggaagggcgc ccaatgcggc
     2161 ccgaccgcct ccaggtgctg ccggagcgcg gcgcggacat cgggacggat gcagtccaca
     2221 tggatgtgga gctggttctg cgaccggccc agggcggagt tgatggccag cgacagcacg
     2281 tcgcgcggca cggcccggcc cacccgctgc tccaggaaat gccgggcctg ccaggcggcc
     2341 tgccagtaat tcggcgcgcc gggggcgagg atgctcctgt cgtcgatgcc gctgacccgg
     2401 gcggtcggga tcagcaggaa ctggtatggc ccgcgccggt ccttcagcac cgcatagccg
     2461 ccttcgaggt tcactgcggc acagggcgcc ggattgtgct tctgcaccag atcgggaacg
     2521 cattttccat gcacgatgtg ccacagcgcg gagggatccg ccgccgcgcg cccggcgagt
     2581 ccgaagctga ggaggagggc gagcagggca agacgaccga gggggggtct cgagtgtcgg
     2641 gatgggttca ttgcggctct ctccggcgca caagcagaat tcagacggcc gggctcgcgg
     2701 cgccgggctc gcctgcatag ctgaaacagt ccgtgcctgc ccaaccattc ggtgccgcaa
     2761 ggtgtccctg ttctccctcc acggcctggc ccacctcaca cagagctacg ggccctggct
     2821 gctcggcgtg gtcgtgatgc tggaggccat gggcctgccg ctcccggccg agagcctgct
     2881 gatcgcgacc gccgccttcg ccggcagctc ccaccatctc gccgtggcct ggattgtcat
     2941 cccggcggcg gcgggggcca tcgtcggcga caatctggga tacctgatcg gccgctcgct
     3001 gggctggcgc gtgctgcggc gctggggccg gcatatcggg ctgaccgacg accgtctgac
     3061 cctgggcgcc tatctgttcc gctgccacgg cggcaaggtc gtgttcttcg gccggttcgt
     3121 cgccgtgctg cgcacgctgg ccgccctgct ggccggcgcc aaccggatga actggtggtg
     3181 gttcctgccc tgcaatgccg ccggcggcat cctgtggagc tgcgtctatg gcctgggcgc
     3241 ctattggctc ggcaaggagg tggagcgcgt cgccaggccg gccggcttcg ccatcggcct
     3301 cctggccgcc gcggtgatcg tcaccgtgtt cctgctgatc cgccgccacg agcaggaact
     3361 gaccgcccgc gccaagcggg agatgcgcgg cgcctgaggc caggagcggt ttcaggtgcc
     3421 ggccccgcat gctacacgac accgcgacac agtaatggcc gcatgatccc aatgaacgag
     3481 cagagcgccc gcgccagcca acaccgattc ttcactgccc gggtggcgga taccgacccg
     3541 gaactcgccg ccgccctgga tggcgaactc gcccgccagc aggacggcat cgagctgatc
     3601 gcctcggaga acatcgtttc cgccgcggtg ctggaggcac agggatcgac gctgaccaac
     3661 aagtatgcgg aaggctatcc cggccgccgc tattacggcg gctgcgttca ggtggacatc
     3721 gccgaacggc tggagatcgc gcgggcgcag caactgttcg gctgcggctt cgccaacgtg
     3781 cagccgcatt ccggcgcaca ggccaaccag tcggtgttcc tggcgctgct gcagccgggc
     3841 gacaccatcc tgggcatgag cctcgccgcc ggcggccacc tgacgcatgg cgccggtccg
     3901 aacctgtccg gcaaatggtt tcgcgccgtg cagtacggcg tgcgccgcga cgacgggctg
     3961 ctggactatg acgagctgga acgcctggcg cgcacggaaa agccgaagct gatcatcgcc
     4021 ggcggctccg cctatccgcg cttcatcgac ttcgcccgca tccgccgcgt cgccgacgag
     4081 gtgggcgcgt atttcatggt ggacatggcg catttcgccg gcctggtggc ggcggagctg
     4141 tttccctcgc cgctgccgca tgcccacgtg gtcaccacca ccacgcacaa gacgctgcgc
     4201 ggcccgcgcg gcggcatggt gctgtcgaac gacccggaga tcggcaagaa gatcaacagc
     4261 gcggtgttcc ccggcctgca aggcggcccg ctgatgcacg tgatcgccgc caaggcggtg
     4321 gcgttcggcg aggcgctgcg cccggagttc cgcctctacc agaagtccgt ggtggagaac
     4381 gcccgcgtgc tggcggaaac cctgcagcaa gccggtctgg ctatcgtcac cggcggcacc
     4441 gactgccacc tgatgctggt cgatctgcgt ccaaagcgcg tcaccggcaa ggcggcggaa
     4501 gccagcctgg agcgcgcgca tatcacggcc aacaagaacg ccatcccgtt cgatccggaa
     4561 aagccggcca ttacctccgg catccggctc ggctcgccgg ccgcaaccac gcgcggcttc
     4621 ggggcggcgg aattccgcga ggtggggaac atgatcaacg aggtcctgga aggcctggcc
     4681 ggctccaacg acggcagcaa cgacgccgcg gaacgcgcgg tgggtgagcg ggtgcgggcg
     4741 ctgtgcgcgc gcttcccgct gtacaacgcg ggctgagccg ccatgcgctg cccgttctgc
     4801 ggccatgagg acacccaggt gaaggacagc cgcccgtccg aggacggcag cgcgatccgc
     4861 cgccgccgct tctgcggcaa ttgcggccag cgcttcacca cggtggagcg cacccagttg
     4921 cgcgaactga cggtggtgaa gcaggacaac cgccgcgtgc cgttcgaccg cgacaagctg
     4981 gcccggtcca tccgcatcgc gctgcgcaag cggccgatcc aggaggagcg gatcgagcgc
     5041 atcgtcaact cgatcgtgcg caagctggaa gcctcgggcg acgcggatat ccccagcaag
     5101 cagatcggcg agctggtgat ggacacgctg aaggaactgg acccggtggc ctacgtgcgc
     5161 ttcgccagcg tctaccgcaa ttttcgtgag gcgaaggatt tcgaggactt cgtcggttcg
     5221 ctgg
//
LOCUS       DASSZH010000656         2719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_885287, whole genome shotgun sequence.
ACCESSION   DASSZH010000656 DASSZH010000000
VERSION     DASSZH010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885287"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /locus_tag="VFN42_14205"
     CDS             complement(<1..1047)
                     /locus_tag="VFN42_14205"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET8997817.1"
                     /translation="METIETLGKYEIKRTLGRGAMGTVYEGWDPVIARRVAIKTVKIP
                     EAEDPETEEQLARFRREAQAAGRLIHPNIVGVFDYGETHDIAYIVMEYVDGPPLKSLL
                     DKQERFTQPNILRIMQDLLTGLQYSHERGVVHRDIKPANLMLTSEGQAKIADFGIARI
                     ESSSMTQAGTLLGTPAYMSPEQFMGQVVDNRTDIYSSGVLLYQLLTGERPFDGGLSAI
                     MHKALNTEPPAPSQISVTCPTAFDLVVRKAMAKRPEDRFQSANEFLQALHAAASGERD
                     DDAYPAEEATLVAAPGSAVRTTISRGEPPAPPAAPAQPPAGRKSLPMIVAAVVVLLLA
                     AGGGGWFFLSRSPVP"
     gene            complement(1075..>2719)
                     /locus_tag="VFN42_14210"
     CDS             complement(1075..>2719)
                     /locus_tag="VFN42_14210"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HET8997818.1"
                     /translation="TTPQLMAPCLDVYARAAQQDPHQRQMLLGEMFTAEQLAQASITS
                     QQIAQASATLAENARDPKVGALIRKERDLKGKLDLLYSQRDEMAQARQQGAPVTPAQT
                     AALDKQVAAIRSQLDDADSALQAASPNYGQLVQEVVPASAVFAQLHPNEAFVALSLAD
                     KDGWVFLLRNHTITVSKIKAGMKDVAGLVQRIRASIELTTSGLPPFDIADAQQLYKLT
                     LGGVAKQLAGVKSVVVAPTGPLLSLPFEVMLTGPAEQGDLAGAPWLMRQYTLAHVPAP
                     SNFVSLRKIAGDSRAAKPWFGFGDFQPVTLAQAEKSFPGSRCADSAQLLAGLPLLPYA
                     GKELTAARELLGASKADELLGAAFTVPAVMRLPLDNYRILQFSTHALLPTDLRCQNQP
                     AIVTSNPAGATDAKNALLTSADVMQLKLDADLVILSACNTGGPGGSTAGESLSGLARS
                     FFYAGARSLLVTHWDVNDQVAAFLVADTLARMRQNPALGVTGALRDAELSMLAQAGKG
                     LPPEIAHPFFWAPFAVIGDGGERLKTVQQAGPSQRFAGL"
BASE COUNT          418 a          931 c          917 g          453 t
ORIGIN      
        1 cggcaccggg ctgcgcgaca ggaagaacca gccgcccccg ccggcggcga gcagcagcac
       61 caccaccgcc gcgacgatca tcggcaggga tttccgcccg gccggcggct gggccggagc
      121 cgccggcggc gccggcggtt cgccgcggga gatcgtggtc cggaccgccg atccgggcgc
      181 ggcgaccagc gtcgcctcct cggcggggta ggcgtcgtcg tcccgctcgc cagacgctgc
      241 agcatgcagc gcctgcagga attcgttggc cgactggaac cggtcctccg gccgcttggc
      301 catcgccttg cgcaccacca ggtcgaatgc ggtcggacag gtgacggaaa tctgcgacgg
      361 cgctggcggc tcggtgttca gcgccttgtg catgatggcc gacaggccgc cgtcgaacgg
      421 ccgctcgccg gtcagcagtt ggtacagcag cacgcccgag gaatagatat cggtgcggtt
      481 gtccaccacc tggcccatga actgctcggg cgacatgtag gcaggtgtgc ccagcaacgt
      541 gccggcctga gtcatcgagc tgctctcgat ccgcgcgatg ccgaaatcgg cgatcttcgc
      601 ctggccctcg ctggtcagca tcaggttggc cggcttgatg tcgcggtgca ccaccccgcg
      661 ctcgtggctg tactgcagcc cggtcagcag gtcctgcata atgcgcagga tgttgggctg
      721 ggtgaaacgc tcctgcttgt ccagcaacga cttcagcggc ggcccatcga catattccat
      781 gacgatgtag gcgatatcgt gggtctcgcc gtaatcgaat acgccgacga tgttgggatg
      841 gatcagccgg ccggcggcct gcgcctcacg ccggaagcgg gcgagttgct cctcggtctc
      901 cggatcctcg gcttccggga ttttcaccgt cttgatcgcc acgcgccggg cgatgaccgg
      961 atcccagccc tcgtacacgg tgcccatcgc accgcgcccc agcgtgcgct tgatctcgta
     1021 cttgccgagg gtctcgatcg tctccatcca cccgtccgcc gtccttcgcg cggactatag
     1081 gccggcaaac cgttgtgatg gaccagcttg ttgcaccgtc ttcagacgct ccccgccgtc
     1141 gccgatcacc gcgaaggggg cccagaagaa gggatgcgcg atttccggcg gcagtccctt
     1201 gccagcctgg gccagcatcg acagttcggc gtcgcgcaag gcgcccgtaa cgcccagcgc
     1261 tggattctgc cgcatgcgag ccagcgtatc cgccaccagg aaggctgcca cctgatcgtt
     1321 gacgtcccag tgcgtcacca gcagcgagcg cgccccggca tagaagaagg aacgcgccag
     1381 tccggacagg ctttcaccgg cggtggaccc gccagggcct ccggtgttgc aggcggacag
     1441 gatcaccagg tcggcgtcca gcttcaactg catcacgtcg gccgaagtca gcagcgcgtt
     1501 cttggcgtcc gtcgcacccg ccggattgga ggtcacgatc gccggctggt tctggcagcg
     1561 caggtcggtc ggcagcaagg cgtgggtgga gaattgcagg atgcggtaat tgtccagcgg
     1621 cagccgcatg accgccggca cggtgaaggc ggctccgagc agctcatcgg ccttgctggc
     1681 ccccagcagc tcacgcgccg cggtcagctc cttgccggca tagggcagca gcggcagccc
     1741 ggccagcaac tgcgcgctgt cggcgcagcg cgaaccggga aagctctttt ccgcctgcgc
     1801 cagcgtcacc ggctggaaat cgccgaaacc gaaccacggc ttggcggcgc gtgagtcccc
     1861 ggcgattttg cgcagcgaca cgaagttgct gggcgccgga acgtgcgcca gggtgtactg
     1921 gcgcatcagc cagggcgcgc cggccagatc gccctgttcc gccggaccgg tcagcatcac
     1981 ctcaaacggc agcgacagca gcggaccggt cggcgccacg accaccgatt tcactcccgc
     2041 aagctgcttc gccacgccac ccagcgtcag cttatagagc tgctgggcgt ccgcgatgtc
     2101 gaagggcggc aggccgctgg tggtcagctc gatgctggcg cggatgcgct gcaccaggcc
     2161 ggcgacgtcc ttcatgccgg ctttgatctt cgacaccgtg atggtgtggt tgcgcagcag
     2221 gaagacccag ccgtccttgt cggccaggct gagcgccacg aaagcctcgt tcgggtggag
     2281 ctgcgcgaac accgcggagg cgggcaccac ctcctgcacc aactggccgt aattcggcga
     2341 ggcggcctgc aaggccgaat ccgcgtcgtc caactgtgag cggattgcgg cgacctgctt
     2401 gtccagcgcg gcggtttgcg ccggggttac cggggcgccc tgctggcgtg cctgcgccat
     2461 ctcgtcgcgc tgactgtaca gcaggtccag cttgcccttc aggtcgcgtt ccttgcggat
     2521 cagggcgccg accttgggat cgcgcgcgtt ctccgccaaa gtggcgctgg cctgcgcgat
     2581 ctgctggctg gtgatgctgg cctgcgcgag ctgctcggcg gtgaacatct cacccagcag
     2641 catttgccgc tggtgcgggt cctgctgcgc tgcccgggca tacacgtcca ggcacggggc
     2701 catgagctgc ggcgtcgtg
//
LOCUS       DASSZH010000657         2368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_783097, whole genome shotgun sequence.
ACCESSION   DASSZH010000657 DASSZH010000000
VERSION     DASSZH010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783097"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1368)
                     /locus_tag="VFN42_14215"
     CDS             complement(<1..1368)
                     /locus_tag="VFN42_14215"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET8997819.1"
                     /translation="MRALPALVLLLLPLAACQTPPAKDYAHGTFGAAHPAAQVSIGRN
                     AVGEECTQAAGEGRSADIYCGTWAQPSARVRQGGPAGAQQLMNLATASPWRAGLDTRF
                     RCEAPQTTTILNNSPAALMQCTQLVGGWAHVALVALVNGHAWYADGVLPAAKVMEHSI
                     GVLSGVATANATPVSSAADALLARRLAAQAFSSGDIGQYNALMSAGTRANLADNPGAA
                     ENAFRAALAIQQKALGKNNPNTVTAMMSLALELSDEGRFADADSLFDQAEALVPRSDD
                     PTAQPRLVHYRGLDALNQNKLEDAEKLLTQADALYAAQVPQSALKARPVAQVGNRFLR
                     IPVSTSDLMPSQELLTDPRAQSALLGLVEVRRYRSIVLRLLGKPKQADALLQSATDLA
                     RGNGLARPMLTARLYRTSALTAEAQGLSSQALSDLSRSTEAFSRALPGSKPLAETYLL
                     HVRELE"
     gene            complement(1371..>2368)
                     /locus_tag="VFN42_14220"
     CDS             complement(1371..>2368)
                     /locus_tag="VFN42_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997820.1"
                     /translation="SASGNLIANLGSFTASSGNFTLINGEDLAVMGPVAANTGSVVIT
                     ATHFGANAPSSLVNQSTIQGQTGVTLVADVNATNSGTIQSSGNDVHVTANSGTLSTSG
                     LINAGRLMVLEALNGPTMTLGGILSAPTIQAKAGPNLLTLNSGLSIITEGTIRQTGTF
                     QKLPPDAFTTKGAYFSSGSFQQLGSGTVSTLAGTGNVVRVDVTQGPLTFSSSGLNAPD
                     TWLILNGSTGAISGAITVKALDVLNNGGSGSANLSGTINGYTGHAAAGQANIEPTRNA
                     KYHVNSCPIHSVNCVLLPTEGVPAANPLDEIFLGSFFNQQDDGDLLLPIVSDQDY"
BASE COUNT          358 a          768 c          836 g          406 t
ORIGIN      
        1 ctccaactcc cgcacgtgca gcaggtaagt ctcggccagc ggtttggaac cgggcagggc
       61 tctgctgaac gcctccgtgg accggctgag atcgctgagc gcctggctgg aaagcccctg
      121 cgcctcggca gtcagcgcgg aggtacggta cagccgggcg gtcagcatcg gtcgcgccag
      181 cccattgccg cgtgcgaggt cggtggccga ctgcagcagc gcgtcggcct gcttcggctt
      241 gcccagcagc cgcagcacga tactgcgata ccgccgcacc tccaccaggc ccagcagcgc
      301 cgactgggcg cgcggatcgg tgagcagctc ctggctcggc atcagatcgc tggtgctcac
      361 cgggatgcgc aggaagcggt tgccgacctg ggccaccgga cgagccttca gcgccgattg
      421 cggcacctgc gccgcataca gcgcatcggc ctgggtcaac agtttttccg cgtcttccag
      481 cttgttctgg ttcagcgcgt ccaggccgcg gtagtggacc agccgaggct gggcggtggg
      541 atcgtcggat cgcggcacga gcgcctcggc ctggtcgaac aggctgtcgg catcggcaaa
      601 gcgcccctcg tcggacaatt cgagggcgag cgacatcatc gcggtgacgg tgttggggtt
      661 gttcttgccc agcgccttct gctggatcgc cagtgccgcg cggaaggcat tctccgccgc
      721 acccgggttg tcggccaggt tcgcccgggt gccggccgac atcagcgcgt tgtattggcc
      781 gatatcgccg gaactgaagg cttgcgccgc cagccgacgc gccagcagcg cgtccgccgc
      841 ggaactcacc ggcgtggcat tcgcggtggc cacaccggac agtacgccga ttgaatgctc
      901 catcaccttg gcagcgggca gcacgccgtc ggcgtaccag gcatggccat tcaccagcgc
      961 gacgagcgcg acgtgcgccc agccgccgac caactgggtg cactgcatca gcgcggccgg
     1021 gctgttgttg aggatcgtgg tggtctgcgg tgcctcgcag cggaagcgcg tgtccaggcc
     1081 ggctcgccaa ggcgaggctg tggcgaggtt catcagttgc tgcgcgccgg ccgggccgcc
     1141 ctggcggacc cgcgcgctgg gctgcgccca tgtgccgcaa tagatgtcgg ccgaccggcc
     1201 ttcgccggcg gcttgggtgc actcctcgcc gaccgcgttg cggccgatcg agacctgcgc
     1261 cgccggatgc gcggcgccga acgtgccgtg cgcatagtcc ttggccggcg gcgtctggca
     1321 cgcggcgagc gggagcagca acagcaccag ggcgggaagg gcgcgcatgg ctagtagtcc
     1381 tggtcggaga cgatgggcag cagcaggtcg ccatcgtcct gttggttgaa gaaggagccg
     1441 aggaaaatct cgtccagcgg attggcggcc ggcacgccct cggtgggcag cagaacgcag
     1501 ttcaccgagt ggatcgggca cgaattgacg tgatacttgg cgttgcgggt cggttcgata
     1561 ttggcctggc cggccgccgc gtggccggta tagccgttga tcgtgccgga gagattggcg
     1621 ctgccggaac cgccgttatt cagcacgtct agcgccttca cggtgatcgc ccccgagatc
     1681 gcgccggtcg aaccgttcag gatcagccat gtgtcgggcg cgttcaggcc gctgctggag
     1741 aaggtgagcg gaccctgcgt cacatcgacg cgcaccacgt tgccggtccc ggccagcgtg
     1801 ctgaccgtgc cagacccaag ctgctggaag ctgccggaac tgaaataggc gcccttggtg
     1861 gtgaaggcat ccggcggcag cttctggaac gtgccggtct gccggatcgt gccttcggtg
     1921 atgatgctga ggccagagtt cagcgtgagc aggttggggc cggctttggc ctggatggtc
     1981 ggcgccgaca ggatgccgcc aagcgtcatg gtcgggccgt tcagcgcctc caacaccatc
     2041 aaacgccccg cgttgatcag cccgctggtg ctgagcgtac cgctgttcgc cgtgacatgc
     2101 acatcgttgc cgctcgactg gatcgtgccg ctgttggtgg cgttcacgtc ggccaccagc
     2161 gtgacaccgg tctggccctg gatggtcgat tggttgacca ggctgctcgg cgcattcgcc
     2221 ccgaaatgcg ttgcggtgat cactaccgag ccggtgttgg cggccaccgg acccatgacc
     2281 gccaggtcct cgccattgat cagcgtgaag ttgccgctgc tcgcagtgaa ggatcccagg
     2341 ttggcaatca ggttgccgga cgccgagg
//
LOCUS       DASSZH010000658         3184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_135637, whole genome shotgun sequence.
ACCESSION   DASSZH010000658 DASSZH010000000
VERSION     DASSZH010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135637"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..992
                     /locus_tag="VFN42_14225"
     CDS             141..992
                     /locus_tag="VFN42_14225"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HET8997821.1"
                     /translation="MPQIRRMGPMTGAEITEVDVRTLDEAGFAPIYQAWLDSGVIVVR
                     DQHLTIPHFLDYSRRFGVVVPHPSKSTRHPEYPEITMLGVNKYGPDGKLVEAIYRRGA
                     EGWHTDGAYDEVPFKATQLYALAVPSTGGNTHFSSMYAAYEALPQRLKQVLDGRRGAF
                     IYGGRRRTNTLLNPEDRERPPAFHPMIRTHAETGRRSLYFDPGKILFIEGLEPRESDD
                     VIEELTGRMIAPDAEYTHVWRVGDIVIWDNRCMVHRAAGDYPPEEDRIHWRVSIKEPA
                     ALGQVAA"
     gene            complement(1014..1712)
                     /locus_tag="VFN42_14230"
     CDS             complement(1014..1712)
                     /locus_tag="VFN42_14230"
                     /inference="COORDINATES: protein motif:HMM:NF020475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HET8997822.1"
                     /translation="MVDYPDPACAYCPPTVRACRAGEADRRGPGFCPSKVDPETQALA
                     QDAYADPETRRLAYHSALVESEGYCRWTRVEEVVHFAKRMGYRKIGIANCISFVDHAY
                     VLTGILESHGFAVVSVACKNGSIPKEELGIRDDEKVRPGGFEALCNPVAQAELLNAHG
                     CDFNVVLGLCIGHDSLFFKYAQAPSTVLVAKDRVLGHNPLAALQLADSYYSRLWGPLH
                     PDKPAKLPPAGRQR"
     gene            complement(1851..2189)
                     /gene="grxD"
                     /locus_tag="VFN42_14235"
     CDS             complement(1851..2189)
                     /gene="grxD"
                     /locus_tag="VFN42_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HET8997823.1"
                     /translation="MASPVHDRIQGDISQNKVMLFMKGTPMFPQCGFSARVVQILSHM
                     AVPFQSANVLEDMELREGIKEFSQWPTIPQLYVDGEFVGGCDIVMEMFQSGELESLLK
                     EKGVPHKAAA"
     gene            complement(2229..2462)
                     /locus_tag="VFN42_14240"
     CDS             complement(2229..2462)
                     /locus_tag="VFN42_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014104720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HET8997824.1"
                     /translation="MSMAANEIEALIKAALPDASVTVEDLAGDGDHYAARVVSAQFRG
                     LPRVRQHQIVYAALRGRMGGELHALALQTSAPE"
     gene            complement(2495..>3184)
                     /locus_tag="VFN42_14245"
     CDS             complement(2495..>3184)
                     /locus_tag="VFN42_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HET8997825.1"
                     /translation="EIMGQFAAAIRGMAAACEALEFPVVSGNVSLYNETEGKAILPTP
                     AIGGLGVLDDARKAVGIALPPWLDVVLIGATKGWLGQSLWLREIAGREEGAPPPVDLP
                     TERTNGDFVRAQIQAGIVRACHDVSDGGILVALAEMAMAGSTGIRVFTHPRDIPGHAF
                     WFGEDQGRYVIAVPDAAAVVRAADAVGVPAMRIGTSGGQDLTLPDGGTISVAALRAAH
                     ERFLPMWLDGT"
BASE COUNT          530 a         1020 c         1114 g          520 t
ORIGIN      
        1 gccaagtgtt taaggcgggc ttaacgggcg cttcattgac gccccttcag ccggcaccta
       61 gggttttgcc aagtgcacgc aggcggccgc cgcgggtgcg cctcgcggcg attgcgcaaa
      121 agaatcagga ggatcgtccg atgccgcaga ttcgccgcat gggtccgatg accggggcgg
      181 aaatcaccga agtggacgta cggacgctgg acgaagcggg gttcgcgccg atctaccagg
      241 cgtggctgga ctccggcgtg atcgtggtgc gcgaccagca tctgacgatc ccgcacttcc
      301 tggactacag ccgccgcttc ggcgttgtag tgccgcatcc gtcgaaatcc acccgccatc
      361 cggagtatcc ggagatcacc atgctggggg tcaacaagta cggcccggat ggcaagctgg
      421 tggaggcgat ctaccgccgc ggcgccgaag gctggcacac cgacggcgcc tatgacgagg
      481 tgccgttcaa ggcgacgcag ctctatgccc tggcggtgcc gagcaccgga ggcaacacgc
      541 atttctccag catgtacgcg gcgtatgagg cgctgccgca gcgcctgaaa caggtgctgg
      601 acgggcgtcg cggcgccttc atctatggcg gacgccgccg gacgaacacc ttgctgaacc
      661 cggaggatcg cgagcggccc ccggcgttcc atccgatgat ccgcacgcac gcagaaaccg
      721 ggcggcgatc gctatatttc gatccgggca agatcctctt catcgagggg ctggagccgc
      781 gcgaaagcga tgatgtcatc gaggaactca ccgggcggat gatcgcgccg gatgccgagt
      841 acacgcatgt ctggcgggtg ggcgacatcg tgatctggga caatcgctgc atggtgcacc
      901 gggcggcggg cgattacccg cccgaggagg accgcatcca ctggcgggtg tcgatcaagg
      961 agccggcggc gctgggacag gtcgcggcgt agcgccggcc ggcaacacca gggttaacgc
     1021 tgacgtccgg caggcggcag tttcgcgggc ttgtccggat gcagcggccc ccagagacgg
     1081 ctgtagtagc tgtcggcgag ctgaagcgcg gcaagcggat tgtggccgag cacgcggtcc
     1141 ttggcgacca ggacggtgga cggcgcctgg gcgtatttga agaacaggct gtcgtggccg
     1201 atgcacagac ccagcacaac gttgaaatcg caaccatgcg cattcagcag ttcagcctgc
     1261 gcgacgggat tgcacagtgc ctcgaatcca ccgggacgca ctttctcgtc gtcgcggatg
     1321 ccgagttctt ccttggggat gctgccgttc ttgcaggcca ccgagacgac cgcaaacccg
     1381 tggctttcca ggatgccggt cagcacgtag gcatggtcca cgaagctgat gcagttggcg
     1441 atgccgatct tgcggtaacc catgcgcttc gcgaagtgca cgacttcctc gacgcgggtc
     1501 cagcggcagt agccttcgga ttcgaccagg gcggagtggt aggcgagcct gcgagtctcc
     1561 ggatcggcgt aagcgtcctg ggccagggcc tgcgtctcgg gatcgacctt gctggggcag
     1621 aagcccgggc cgcggcgatc ggcctcgccg gcgcggcagg cgcgcacggt gggcgggcaa
     1681 taggcgcagg caggatcggg gtagtccacc atggccgcgt tcctccgggt ccgggacgca
     1741 gcatgcgggg tggagggcgg cggaggcttt gattcaggtc aaaggggggc acaagcgttt
     1801 cccccctccc gcaaggggag ggggaatttt ctcgcctcgc cttacgcggg ttacgccgcc
     1861 gccttgtgcg ggacgccctt ttccttcagc agcgactcca gctcgccgga ctggaacatc
     1921 tccatgacga tatcgcagcc accgacgaac tcgccatcca cgtagagctg cgggatggtc
     1981 ggccactggg agaattcctt gatcccctcg cgcagctcca tgtcctccag cacgttcgcc
     2041 gactggaacg gcactgccat gtggctgagg atctgcacca cgcgggccga gaagccgcat
     2101 tgcgggaaca tcggcgtgcc cttcatgaag agcatcacct tgttctggct gatgtcgccc
     2161 tggatgcgat cgtggacggg gctggccatg gtcatgggac tccttggatc aatactggcc
     2221 ggcgcgggtc attccggcgc cgaggtttgt agcgcgaggg cgtgcagctc gccccccatg
     2281 cggccacgca acgcggcata gacgatctgg tgctgcctca cgcgcggcag gccgcggaac
     2341 tgtgcggata cgacccgggc ggcatagtgg tcgccgtcgc cggccaggtc ctccaccgtt
     2401 acgctggcgt cgggcagggc cgccttgatc agggcctcga tctcgttcgc cgccatcgac
     2461 atgcaggtga gtctccccag ggtcggttgc cgtatcaggt gccgtccagc cacatcggca
     2521 ggaagcgttc gtgcgccgcg cgcaacgccg ctacggatat tgtgccgccg tccgggagtg
     2581 tcaaatcctg gccaccggaa gtgccgatgc gcattgccgg cacgcccacg gcgtcggccg
     2641 cgcggaccac cgcggcggcg tccgggacgg cgatgacata gcggccctga tcctcgccga
     2701 accagaaggc gtggccggga atgtcgcgcg gatgggtgaa cacgcggatg ccggtgctgc
     2761 cggccatcgc catctccgcc agcgcgacca ggatgccgcc gtcggagacg tcatggcagg
     2821 cgcgcacgat gccggcctgg atctgcgcac gcacgaaatc gccgttggtg cgctcggtgg
     2881 gcagatcgac gggcggcggg gcgccttcct cccggccggc gatctcgcgc agccagagcg
     2941 actggccgag ccagcccttg gtggcgccga tcagcacgac gtcgagccag gggggcagtg
     3001 cgatgccgac cgccttgcgc gcgtcgtcca gcacccccag gccgccgatt gccggggtgg
     3061 gcaggatcgc cttgccttcg gtctcgttgt agaggctgac attgcctgac acgacgggga
     3121 attccagcgc ctcgcaggcg gcggccatgc cgcggatggc ggcggcgaac tggcccatga
     3181 tctc
//
LOCUS       DASSZH010000659         2554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1686925, whole genome shotgun sequence.
ACCESSION   DASSZH010000659 DASSZH010000000
VERSION     DASSZH010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686925"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="VFN42_14250"
     CDS             <1..1134
                     /locus_tag="VFN42_14250"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET8997826.1"
                     /translation="QLLQPCLSEADLSLVGRVATRWDVVRFLSNLLQMRDAERRDPAI
                     EQQRVTAPIFITGLPRSGTTFLHRMLMEDPANRAPLVWQAIHPYPPPRGADRRKASVA
                     RQLRTFEWLAPEFRDLHPLKADSPQECSEITAHVFRSLRFDSTYNIPSYRAWLDTDLA
                     GHLPAYRFHRRFLQHLQQADAGWTAETRWVLKCPDHLFALDALRAVYPDARLVFVHRD
                     PVKVLLSVAKLTEVLRRPFSRHVDPLAIGRQESARWFDGTRRMIAASDADGDSAICHV
                     HYLDLVSDPVATVEQVYQHFDMALSPAAAQAMARYVAARPNGGYGPHRYRFEDHGLDP
                     DAERAKFRPYMLHFGIEAEAAPDGPRRAAPQKQTQGALPPGAG"
     gene            complement(1186..1479)
                     /locus_tag="VFN42_14255"
     CDS             complement(1186..1479)
                     /locus_tag="VFN42_14255"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HET8997827.1"
                     /translation="MSDRVLNLMSSAVARQTEALPARTRRSWIPGMAGFGAGRSGWPV
                     QTGPQGLGAWLRAAWRRHRSRQQIARLDAQTLRDIGVSYAEAENEANKPFWRR"
     gene            1585..2505
                     /locus_tag="VFN42_14260"
     CDS             1585..2505
                     /locus_tag="VFN42_14260"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET8997828.1"
                     /translation="MSTLQGLDADLLRVFVLIAEGHSFTQAAALVGRTQSAVSMQIRR
                     LEDLLGQPLLLRAKGGTVTLTPHGSYLLTRARQMLALNDEVMATFRTPEIAGTVRLGT
                     PDDYAFAYLPPVLKRFAETHPAVQVEVQCSPSEDLVQAIRAGELDLSLISDGLQPPGW
                     PVVPLWRGPLVWVTSTRYAPHRQDPLPLALADREKFLAAERDCEWARAALNALEQAGR
                     RYRIAYTSASQVGTHAPVLAGLAVTVSSLSWLPEGLRPVRPDEGLPPLPEFRLMLMKA
                     KLPRQPVTDALAAHIEASVRPEQSGALAAE"
BASE COUNT          379 a          922 c          857 g          396 t
ORIGIN      
        1 cagttgctgc aaccctgcct gagcgaggcc gatctcagcc tggtcggccg ggtcgccacg
       61 cgctgggacg tggtccgctt cctgtccaac ctgctgcaga tgcgcgatgc ggaacgccgc
      121 gatccggcga tcgagcaaca gcgcgtcacc gcgcccatct tcatcaccgg gctgccgcgc
      181 agcggcacca ccttcctgca tcgcatgctg atggaggatc cggccaaccg cgcgccgctg
      241 gtctggcagg cgatccatcc ctacccgccg ccgcgcggtg ccgaccgccg caaggcctcg
      301 gtggcgcgcc agttgcgcac cttcgaatgg ctggcgccgg agttccgcga cctgcatccg
      361 ctgaaggcgg attcgccgca ggaatgcagc gagatcaccg cgcatgtgtt ccgcagcctg
      421 cgcttcgatt ccacctataa catccccagc taccgcgcct ggctggatac cgacctggcg
      481 ggccacctgc cggcctaccg cttccaccgg cgcttcctgc aacacctgca gcaggcggat
      541 gccggctgga cggccgaaac gcgctgggtg ctgaaatgcc ccgaccacct gttcgcgctg
      601 gatgcgctgc gcgcggtgta tcccgatgca cggctggtct tcgtgcatcg cgatccggtg
      661 aaggtgctgc tgtcggtggc caagctgacc gaggtgctgc gccgcccgtt ttcccgccac
      721 gtcgatccgc tggcgatcgg ccggcaggaa agcgcccgct ggttcgacgg gacacggcgg
      781 atgatcgccg ccagtgacgc cgatggcgat tcggcgatct gccacgtgca ctacctggac
      841 ctggtttccg atccggtcgc cacggtggag caggtgtacc agcacttcga catggcgctc
      901 agccccgccg cggcgcaggc catggcgcgc tacgtggcag cacggccgaa cggcggctac
      961 ggcccgcacc gctaccgctt cgaggatcac ggcctggatc cggacgcgga gcgggcgaag
     1021 ttccgcccct acatgctgca tttcgggatc gaggcggaag cagcccccga tggcccccgc
     1081 cgggccgcac cgcagaagca gactcagggc gccctgcccc cgggcgccgg ctgaccggtt
     1141 gcgctccttg gcagcatggc atggctgccg gggagcgctg ccggatcagc ggcgccagaa
     1201 cggcttgttc gcctcgttct ccgcctcggc atagctcacc ccgatatcgc gcaacgtctg
     1261 cgcatccagg cgcgcaatct gctggcggga gcggtggcgc cgccacgccg cgcgcagcca
     1321 ggcgccgagg ccctgcggtc cggtctgcac cggccaaccc gagcggccgg cgccgaaccc
     1381 ggccatgccg ggaatccagg atcggcgcgt ccgcgccggc agcgcctcgg tctgccgagc
     1441 gaccgcggat gacatgagat tcagcacacg atcggacatg gcggcctcct tgccccataa
     1501 ccattgctgc aacggaatct atggccgctg ctggattagt aaaacgaatt gccttgatgt
     1561 gggtgatcag cgatcatgat gaccatgagc acgctccagg gtctcgacgc cgatctgctg
     1621 cgggtcttcg tgctgatcgc cgaaggccac agtttcaccc aggccgcggc gctggtcggc
     1681 cggacccagt ccgccgtctc catgcagatc aggcggctgg aggatctgct cggccagccg
     1741 ctgctgctgc gcgcgaaggg cggcaccgtc acgctgactc cgcatggcag ctacctgctg
     1801 acccgcgccc ggcagatgct ggcgctcaat gatgaggtga tggcgacgtt ccggaccccg
     1861 gaaatcgccg gcaccgtccg gctgggcacg ccggacgact acgccttcgc ctacctcccc
     1921 ccggtgctga agcgcttcgc cgagacgcat cccgcggtcc aggtggaggt gcaatgctcg
     1981 ccgtccgagg atctggtcca ggcaatccgc gccggggaac tcgacctcag cctgatctcc
     2041 gatggcctgc agccacccgg ctggcccgtc gtgccgttgt ggcgcggccc gctggtctgg
     2101 gtcacctcca cgcgctacgc cccgcaccgg caggacccgc tgccgctggc gctggccgac
     2161 cgggagaagt tcctggcggc ggaacgcgat tgcgaatggg cgcgcgccgc gctgaacgcg
     2221 ctggaacagg cggggcggcg ctaccgcatc gcctacacct ccgcttccca ggttggcacg
     2281 catgcgccgg tgctggccgg cttggcggtg accgtgtcca gcctgtcctg gctgccggaa
     2341 ggattgcgcc cggtgcggcc ggacgaaggc ttgccgccgc tgccggagtt ccgcctgatg
     2401 ctgatgaagg cgaagctgcc gcgtcagccg gtcaccgatg cgctggccgc gcatatcgag
     2461 gcgagcgtca ggccagagca gagcggcgcg ctggcggcgg agtagcgcaa gccccgggca
     2521 ttaatccggc gctaagccgc gccgccgata ctcc
//
LOCUS       DASSZH010000660         2251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_509895, whole genome shotgun sequence.
ACCESSION   DASSZH010000660 DASSZH010000000
VERSION     DASSZH010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509895"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VFN42_14265"
     CDS             complement(<1..375)
                     /locus_tag="VFN42_14265"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET8997829.1"
                     /translation="MSVVTTEQRGPISIIRINRPERLNAISSAVAVELQQAFQAFDAS
                     DQRVAVLSSVGGRAFTSGADVSDLPELWRAIPGVGFQTDKPIIAATSGWVVGGGIVLV
                     MMCDLMVSTEDTIFYYPEARLGV"
     gene            complement(396..1271)
                     /locus_tag="VFN42_14270"
     CDS             complement(396..1271)
                     /locus_tag="VFN42_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HET8997830.1"
                     /translation="MNAPRNTQYRTLEVRRIAGSLGAEIDGVDLSQDLPQDVLAELRA
                     ALLDNLVIFFRNQNLTPEQQLRFARRWGDIHLHPFMQGMAAYPEVLEIIKTPTDKKNF
                     GGSWHTDQMFSPQPAMGTILYAKEVPSAGGDTMFTNQYLAYESLSGGMQRIVEGLRTV
                     CVGDNFKQHGGMSRAQRYAGQMAHMKVKDPGNVQTTSVHPLVRTHPETGRKALYIGGH
                     VQRFDGMTDEESEPLIQYLKQHSTRPEFTCRFRWETGSLAFWDNRCTQHFAINDYPAE
                     TRIMHRITVCGDTPF"
     gene            1457..1927
                     /locus_tag="VFN42_14275"
     CDS             1457..1927
                     /locus_tag="VFN42_14275"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997831.1"
                     /translation="MSRDRLFGAISVTALVLLGFGATAWSASLTITSRHKAPLGEYLT
                     DGHGRTMYMFTADKGKHSACDADAACTKAWPPVLTNGAIMPGDAVDAGELGTIPHGKK
                     IGQVTYHGMPLYYFVRDKKPGSVAGEGINHFGGSWYVVSPKGHPVQPNGKQLGG"
BASE COUNT          364 a          744 c          757 g          386 t
ORIGIN      
        1 gacgcccagc ctggcctccg ggtagtagaa gatggtgtcc tcggtggaga ccatcaggtc
       61 gcacatcatc accagcacga tgccgccgcc caccacccat ccggacgtcg cggcgatgat
      121 cggcttgtcg gtctggaagc caacgccggg gatggcgcgc cacagctccg gcaggtcgct
      181 gacatcggcg ccggaggtga aggcgcggcc gccgacggag gacagcaccg ccacgcgctg
      241 gtccgacgcg tcgaacgcct ggaaggcctg ctgcagctcc acggcgacgg cgctgctgat
      301 cgcgttcagg cgctccggcc ggttgatgcg gatgatggag atcgggccgc gctgctcggt
      361 ggtgacgacg ctcatcctgc tctcctgtgc cgcgctcaga acggcgtgtc gccgcagacg
      421 gtgatgcggt gcatgatgcg ggtttccgcc ggatagtcgt tgatcgcgaa atgctgcgtg
      481 cagcggttgt cccagaaggc gagcgagccg gtttcccacc ggaagcggca ggtgaattcc
      541 ggccgggtgg aatgctgctt caggtactgg atcaacggct cgctttcctc gtcggtcatg
      601 ccgtcgaagc gctgcacatg gccgccgata tacagcgcct tgcggccggt ctccggatgc
      661 gtgcgcacca gcggatgcac cgaggtggtc tgcacgttgc ccgggtcctt caccttcata
      721 tgcgccatct gcccggcata acgctgggcg cgcgacatgc cgccatgctg cttgaagttg
      781 tcgccgacgc acaccgtgcg cagtccttcg acgatccgct gcatgccgcc ggacagcgat
      841 tcgtaggcga gatactggtt ggtgaacatg gtgtcgccac cggccgacgg cacttccttg
      901 gcatacagga tcgtgcccat cgccggctgc ggcgagaaca tctggtcggt gtgccacgac
      961 ccgccgaagt tcttcttgtc ggtgggcgtc ttgatgatct ccagcacctc cggatacgcc
     1021 gccatgccct gcatgaacgg gtgcaggtgg atatcgcccc agcggcgggc gaagcgcagt
     1081 tgctgctccg gagtcaggtt ctggttgcgg aagaagatca ccaggttgtc gagcagggcg
     1141 gcgcgcagtt ccgccagcac gtcctgcggc aaatcctgcg acagatccac gccatcgatc
     1201 tcggccccca gggaaccggc gatccggcgg acttccaggg tgcgatactg cgtgttgcgc
     1261 ggcgcattca ttggaccggc cctcctgacc tgttgcggaa agcatgcccc cggccgcacg
     1321 gcctgggaag tggccgaccc gcgccagccg ggcttgccga gccttagggt tttggtaacg
     1381 cgggcacaat tgacgcggcg ggcgtcatgg gccatccctg cgcgtccctg catgtttcac
     1441 caacaggaga gaacgcatgt cgcgcgaccg tcttttcggc gccatctctg tcacggctct
     1501 tgtcttgctt ggcttcggcg ccactgcctg gtcggcatcg ctgacgatta catcgcgtca
     1561 caaggcgccg ctgggcgaat acctgaccga cggtcacggc aggaccatgt acatgttcac
     1621 cgccgacaag ggcaaacaca gcgcctgcga tgccgatgcc gcctgcacca aggcatggcc
     1681 cccggtgctg accaatggcg ccatcatgcc cggcgatgcg gtcgatgcgg gcgagctggg
     1741 caccatcccg cacggcaaga agatcggcca ggtcacctat cacgggatgc cgctgtatta
     1801 cttcgtgcgt gacaagaagc cgggctcggt ggccggcgag ggcatcaacc acttcggcgg
     1861 ctcgtggtac gtggtgtcgc ccaagggcca tccggtgcag ccgaacggca agcaactcgg
     1921 cggctagcct ggccgggggg aggcccacgc ttccctcctc gccctggtgc cgcggctgcc
     1981 agtccggcac atggcagatg tcaagccgcg ggcggttgcg cgcgaagagg gctgctgtat
     2041 cagtgggcgt gtccagccca ggcagacagg aggccgtttg accgaggcac cgcccgccga
     2101 cctgcgcacc ccggaacaga gcgtgccgct cgactgggcc tcggtgcgcc tgcatctggc
     2161 cgaacacggc atgcggctgg acgccgaccc gccgccgcgg cagttcgccg gcgggctggc
     2221 caacctgaat tacctcgtgc aggtggacgg c
//
LOCUS       DASSZH010000661         3176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_646676, whole genome shotgun sequence.
ACCESSION   DASSZH010000661 DASSZH010000000
VERSION     DASSZH010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646676"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VFN42_14280"
     CDS             complement(<1..345)
                     /locus_tag="VFN42_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997832.1"
                     /translation="MSEAIPLLEYFQSGGAVDEAVPAAAPMVESDLFVTAGSGDLQDL
                     IEDPCRSLEVEYKGWRNLNHAEDRAELARDIAALANHGGGYVVFGFCRETLVAEDTHP
                     FWTRCSTEQVAGV"
     gene            complement(486..1757)
                     /locus_tag="VFN42_14285"
     CDS             complement(486..1757)
                     /locus_tag="VFN42_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8997833.1"
                     /translation="MSEQPSVMRAWSDRALWMMGAYGFVSGLPLSLSGFTLRLWLSDS
                     GVSLALIGITANIGLAYSLKFLWAPLLDNVAPPGLLRRLGRRRGWLATIQPALALAAA
                     LLAMSSPHALPLMPIAAGGLVAFLSASQDIVIDAWRIEIFPPRRQGEALAAYVWGYRV
                     ALLIATTGVIAASTRVGWHVALLGLAGLIALGLPLTLLAPEPLVAAKPILSGMAARLR
                     HAVWEPLRDFLTRPGAPLILTFVALFKLGEAMAGIMTAPLYRSLGFSRDAIAATGPFS
                     LAATLAGITLGGWLVARIGVGRALLWTGWVQTLAMGMYLLLALSAGAHVVLYGTVTME
                     AFAQGMADAAFLTYLSGLCSIAFSATHYALLSSLAAIAVHTLGGASGILAMRLGWVHF
                     YGLCMFAALPSMGLMLLLLRRYPPAEHAPRH"
     gene            complement(1854..2372)
                     /locus_tag="VFN42_14290"
     CDS             complement(1854..2372)
                     /locus_tag="VFN42_14290"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8997834.1"
                     /translation="MEIRNATEADVPEIQAIYAHHVLNGTGTFDVEPPSVEEMRERFL
                     KVTHGTGVWLVACDASGVLGYAYYTQFRDRPAYRFCVEDSIYVRDDVRGQGVGKALVG
                     HLVEEAIARGMRQMVAVIGDAENVGSIGAHASSGFQVVGTLRSAGLKFGRWLDVVYMQ
                     RALGRGNADVPD"
     gene            complement(2387..>3176)
                     /locus_tag="VFN42_14295"
     CDS             complement(2387..>3176)
                     /locus_tag="VFN42_14295"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HET8997835.1"
                     /translation="SVRIPASYCGLFGFRPTHGSISMAGACALAPSMDTCGWFTRSAS
                     LLAGIGEVLLPGGPQGADGPLLRPEEPWVNAQPEIAEVLRPALEKLEQLRGRAIGIRA
                     VPEGVDSVFDHFRTVQAEEAWATLGSWVDTIQPRFGPGVGERFAAAKATDSAIASPGR
                     AFRRTLQARLRPLLAGGAVMVYPTSPCVAPLLTASLEEQDIVRQASIGVTAIASLCGL
                     PEVTLPAGSAHGAPVGLSLVAGVGHDRALLALACDVAAVLGLTV"
BASE COUNT          585 a         1152 c         1015 g          424 t
ORIGIN      
        1 cacaccggca acctgctcgg tggaacagcg cgtccagaag ggatgcgtgt cttccgccac
       61 cagcgtctcg cggcagaagc cgaacaccac gtagccgccg ccatgattgg ccaatgcggc
      121 gatatcgcgc gccagttccg cgcggtcctc ggcatggttc aggttgcgcc agcccttgta
      181 ctcgacttcc aggctgcggc agggatcctc gatcaagtcc tgcagatcgc cggaaccggc
      241 ggtgacgaac aggtccgatt ccaccatcgg cgcggctgcc ggcacagcct catccacggc
      301 gccgccggac tggaaatact cgagaagggg gatggcttcg gacatctcga actcaacgct
      361 ctcgcggccg attgcagtga tatttgccag ttcttgcaac cgcttagctg cagagattac
      421 accagccgaa ccggccaagg cgttacaaat gcatcaaaaa tatggtcgcg gaacggtctg
      481 tggctttaat gccgcggtgc gtgctccgcc ggggggtagc gcctcagcag cagcaacatc
      541 agccccatcg acggcaaggc cgcgaacatg cacagcccgt agaaatgcac ccagcccagc
      601 cgcatcgcca gaattccgga tgcaccgccc agcgtgtgca cggcgatcgc ggccagcgat
      661 gaaagcagcg cgtaatgcgt cgcgctgaag gcaatggagc acaatccaga caggtaggtc
      721 aggaacgccg catccgccat gccctgcgcg aaggcttcca tcgttaccgt tccgtacagg
      781 acgacatgcg cgccggccga cagcgccagc agcaggtaca tccccatcgc cagggtctgc
      841 acccagccgg tccacagcag cgcgcggccg acgccgatgc gtgccaccag ccagccgcca
      901 agggtgatcc ccgccagcgt cgccgccaga gagaacggtc cggtggcggc gatcgcatcc
      961 cggctgaagc ccagcgagcg gtacagcggc gcggtcatga tgccggccat cgcctcgccc
     1021 agcttgaaca gtgcgacgaa ggtcaggatc agcggcgcac ccgggcgcgt caggaaatcc
     1081 cgcagcggct cccagaccgc gtgccgcaga cgagccgcca tgccggacag aatcggcttc
     1141 gcggcgacca gcggctctgg ggccagcagc gtcaacggca ggcccaacgc gatcaatccg
     1201 gccaacccca gcagcgccac atgccagccc acgcgggttg aggcggcgat cacgccagtg
     1261 gtggcgatca gcagcgccac gcgatagccc cagacatagg ctgccagtgc ctcgccctgg
     1321 cggcgtggcg gaaagatctc gatgcgccag gcgtcgatga cgatgtcctg gctcgccgac
     1381 aggaatgcca ccagcccgcc ggcggcgatc ggcatcagcg gcaaggcgtg cgggctgctc
     1441 atcgccagca gcgccgctgc cagtgccagc gccggctgga tcgttgccag ccagccccgc
     1501 cgccgcccca gccggcgcag cagcccgggc ggcgccacgt tgtccagcag cggcgcccag
     1561 aggaatttca gcgaatatgc caggccgatg tttgccgtga tgccgatcag cgcgagcgac
     1621 acaccgctgt ccgacagcca cagccgcagg gtgaaacccg acagcgacag cggcaggcca
     1681 gagacgaagc cgtacgcgcc catcatccac agcgcccggt cgctccaggc acgcatcacc
     1741 gaaggctgtt cactcattgg caagggtgat gtcggggcgg gtgcaggcct tcgcccgcca
     1801 tgacggatcg cgcgtcatcg cgtgcaactc aacatcattg ccggcttgct gggtcagtcg
     1861 gggacatccg cgttgccgcg ccccagggcg cgctgcatgt agaccacatc cagccaacgc
     1921 ccgaacttca ggccggcact gcgcaatgtg ccaaccacct gaaagccgga gctggcgtgg
     1981 gcgccgatcg aaccgacatt ctcggcatcg ccgatcaccg ccaccatctg gcgcatgccc
     2041 cgcgcaatgg cttcctccac cagatgcccg accagggcct tgccgacgcc ctgcccgcgc
     2101 acgtcgtcgc gcacgtagat gctgtcctcc acgcagaacc ggtaggccgg gcggtcgcgg
     2161 aactgcgtgt agtaggcgta gcccagcacg ccggacgcat cgcaagccac cagccagacg
     2221 ccggtgccat gcgtcacctt caggaaacgc tcccgcatct cctccaccga aggcggctcc
     2281 acgtcgaacg taccggtgcc gttcagcaca tgatgggcgt agatcgcctg aatctccggg
     2341 acatcggctt cggtggcgtt gcggatctcc atggtcccct ctgaagtcaa accgtcaggc
     2401 ccagcaccgc cgccacatcg caggcgagcg ccagcagcgc gcgatcgtgc ccgacaccgg
     2461 ccaccagcga tagcccaacc ggcgccccgt gggcactccc cgccggcaat gtgacctccg
     2521 gcaggccgca caggctggca atcgcggtca cgccgatgct cgcctgccgg acgatgtcct
     2581 gctcctccag gctggctgtc agcagcggcg cgacgcatgg gctggtcggg tagaccatca
     2641 ccgcgccacc ggccagcaac gggcgcaggc gcgcctgcag ggtgcggcgg aacgcccgcc
     2701 cgggcgaggc gatcgccgaa tcggtcgcct tcgccgcggc aaaccgctcg ccgacgccgg
     2761 gcccgaagcg cggctggatg gtgtccaccc aggagccgag cgtcgcccag gcttcctcgg
     2821 cctggacggt gcggaaatga tcgaacacgc tgtccacgcc ttcgggcacc gcgcggatgc
     2881 cgatggcgcg gccgcgcaac tgctccagct tctccagcgc cggccgcagc acctcggcga
     2941 tctccggctg ggcgttcacc cagggctcct ccgggcgcag cagcgggccg tcggcgcctt
     3001 gcgggccgcc cggcaacagc acttcgccaa tgcccgccag cagcgacgcg ctgcgggtga
     3061 accagccgca ggtatccatg ctgggtgcca gcgcgcaggc gccggccatg ctgatggagc
     3121 catgcgtcgg gcggaagccg aacaggccgc aatagctggc cggaatgcgc accgaa
//
LOCUS       DASSZH010000662         6137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1277327, whole genome shotgun sequence.
ACCESSION   DASSZH010000662 DASSZH010000000
VERSION     DASSZH010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6137
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277327"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /gene="purN"
                     /locus_tag="VFN42_14300"
     CDS             complement(<1..624)
                     /gene="purN"
                     /locus_tag="VFN42_14300"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HET8997836.1"
                     /translation="MTARSASRRRTAVLISGRGSNMASLLDAARDPDYPAEIVLVLSN
                     RPDAPGLCLAAEAGVEARAIDHRPFGKDRAAHEVRLDAALRDAGVTLVCLAGYMRLLT
                     PFFVGAWQGRMLNIHPSLLPAFPGLHTHARALAAGVKLHGCTVHLVTEIMDEGPILAQ
                     AAVPVLPGDTEDTLADRVLAQEHVIYPAALQAFAAGTAGMPADAAATL"
     gene            complement(621..1703)
                     /gene="purM"
                     /locus_tag="VFN42_14305"
     CDS             complement(621..1703)
                     /gene="purM"
                     /locus_tag="VFN42_14305"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003625164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET8997837.1"
                     /translation="MTSIDRPDAAGRNRQTYRAAGVDIDAGEALVEAIKPLARATSRA
                     GVMGGLGGFGALFDLKAAGFRDPVLVSTTDGVGTKLKVAIETGIHDTVGIDLVAMCVN
                     DLVVQGAEPLFFLDYFATGKLSVESARQVIAGIAAGCGQAGCALVGGETAEMPGMYAA
                     GDYDLAGFAVGAAERGSLLPAGVAPGDAVLGLASAGVHSNGFSLVRRVVAASGLRWDA
                     PAPFAAGQSLGQALMAPTRIYVRSLLALHRAGLLKAAAHITGGGLPGNVPRVLPEGCV
                     AALDGGAWAVPPVFGWLARTGGIADDEMLRVFNCGLGMVLVVSDAAAALAELRASGEQ
                     ACEIGRIEAASGPAAVRMTAMPDWGA"
     gene            1790..2359
                     /locus_tag="VFN42_14310"
     CDS             1790..2359
                     /locus_tag="VFN42_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET8997838.1"
                     /translation="MPDGSQLSRPASAALVTWPNVITFGRLCAVPLAFWLILDHSMGL
                     AFFLFVAAGASDAVDGWLARRNGGGNSVGAVLDPVADKALLVTMYVTLASVNELPDWI
                     AILVVFRDLVIVGGVLVLAVLGDPVTIRPLYISKLNTAMQLILVAMTLALAGFGLHAE
                     LAKLLLIWIVAATTLSSGAAYVWVTVRGR"
     gene            2356..3534
                     /locus_tag="VFN42_14315"
     CDS             2356..3534
                     /locus_tag="VFN42_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET8997839.1"
                     /translation="MSDIPTLAPDLEVPPPERPPAAGSPGPLPSVARRSPATRAQRIA
                     LIAGLLVVTWLALDLFASVLAPFVAAAVIAYALDPPTTRLTRLGVPRGIAALLMIVAV
                     LAAVLLFLLLLYPLIIAQLGLFLGRIPVYVGELQAWARDTITHLQENLGPDAVNDKLR
                     DLVASQAGNMLTFLVSAITSLIGGGFAIFNVLTLAVVTPVVGFYLLRDWQWAVRQVDS
                     WLPRRYAGVIRAQAREVDRILSAWVRGQALCCVLLALYYTMAFTLAGLDLGLIVGLAA
                     GLLSFIPYVGSITGGLTAIALATAQFPHWRGVIGIVVVLVIGQILEGYVIYPRFLGDR
                     VELPAVWVIFALFAGGAAFGFLGVMLAVPVAATIGVLCRFWLRRYLGSQLYLDPPVRS
                     "
     gene            3636..5366
                     /locus_tag="VFN42_14320"
     CDS             3636..5366
                     /locus_tag="VFN42_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET8997840.1"
                     /translation="MSATIEPSLATARRTPNLWDLAAMLCVFGTLAAVAHVAQGTMVP
                     INAPGATTISLDPRNLPDYAVRTTLRMFAALAASLLFTFAYGTAAAKSRRAGLVLVPL
                     LDILQSVPILGFLSFTVVFFMRLFPGQVFGLELAAIFAIFTSQAWNMAFSLYQSLQTV
                     PPDLTEASRAFQLTPWQRFWRLEVPFAVPGLVWNTMISMSGGWFFVVASEAVSVGNSN
                     WKLPGIGSYVAVALEQRNIAAVGWAIFAMLIVILLYDQLLFRPLVAWSAKFRVETTAG
                     ATAEDPWMLRLLRRTRWIAVAGRRMGDAVAMLGMLRLSFRRQARPAAAGRAPSRLVDV
                     LLLAGLFLVLMWAAGRLSAFVAGELAWSDLGRALLLGAYTLIRVLALVTLATAIWVPA
                     GVWIGLRPVWARRAQPVAQFLAAFPANLLFPPFVLFIVHFRLDPDIWLAPLMILGTQW
                     YILFNVVAAAASFPGDLREAASNFRIHGLLWWRKVIIPGILGSYVTGALAAAGGAWNA
                     SIVAEAASWGNTTLYAHGLGAYIAQATLHGDMQRVVLGVAVMALYVVTLNRVLWRPLY
                     AYAQRRLTLS"
     gene            5382..>6137
                     /locus_tag="VFN42_14325"
     CDS             5382..>6137
                     /locus_tag="VFN42_14325"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014106705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8997841.1"
                     /translation="MDTTATATLLEVQNIRQSYHKDANADLVVLDGVDLTLREREMVA
                     LLGRSGSGKSTLLRIVSGLLPPTRGDVLWRGKKLLGPADGVAMVFQSFALFPWLTVQE
                     NVELGLEARGVPRDERERRAEAAIDLIGLGGFESAYPKELSGGMRQRVGLARALVVHP
                     DLLLMDEPFSALDVLTAETLRTDLIDLWSEGRLPVKSVLMVTHNIEEAVLMCDRILVF
                     SSSPGRVAHELKVPFPHPRNRLDPAFREMVDDIY"
BASE COUNT          793 a         2181 c         2139 g         1024 t
ORIGIN      
        1 cagggtggcc gcggcgtcgg ccggcattcc ggcggttccc gcggcgaaag cctgcagggc
       61 cgccggatag atcacatgct cctgtgccag cacgcggtcg gccagcgtgt cctctgtgtc
      121 gcccggcagc acaggaaccg ctgcctgggc caggatcggg ccttcatcca tgatttcggt
      181 gaccaggtgc accgtgcagc catgcagctt caccccggcg gccagtgcac gggcgtgggt
      241 atgcaggccg ggaaaggccg gcagcaggct gggatggatg ttcagcatgc ggccctgcca
      301 ggcgccgacg aaaaacggcg tgagcagccg catgtagccg gccaggcaga ccagcgtcac
      361 gccggcgtcg cgcaatgccg cgtccagccg gacctcgtgc gcggcgcgat ccttgccgaa
      421 gggccggtgg tcgatcgccc tcgcctccac cccggcttcg gccgccaggc acaggccggg
      481 cgcgtccggg cggttggaga ggaccagtac gatctcggcc gggtagtcgg ggtcgcgtgc
      541 cgcatccagc agcgatgcca tgttgctgcc gcgcccgctg atcagaaccg ccgtgcggcg
      601 gcggctggca ctccgtgcgg tcatgcgccc caatccggca tcgcggtcat ccgcacggca
      661 gccggcccgc tggccgcttc gatgcggcca atttcgcacg cctgctcgcc ggacgcccgc
      721 agttccgcca gcgccgccgc cgcgtcggac accaccagca ccatgccaag cccgcagttg
      781 aacacccgca gcatctcgtc atcggcgatg ccgccggtgc gggccagcca gccgaacacc
      841 ggcggcacgg cccaggcgcc gccgtccagc gccgccacgc agccctccgg cagcacgcgc
      901 ggcacattcc ccggcagccc gccgccggtg atatgcgccg ccgccttcag cagcccggcg
      961 cgatgcagcg ccagcagcga ccgtacgtag atacgggtcg gggccatcag cgcctgcccg
     1021 agactctggc cggcggcgaa gggcgccggg gcgtcccagc gcaggccgct ggccgccacc
     1081 acgcggcgca ccagcgagaa gccgttggaa tgcacaccgg cgcttgccag gccgagcacc
     1141 gcatcgcctg gcgcgacacc ggccggcagc agggagccgc gctccgccgc gcccaccgca
     1201 aagccggcca ggtcgtagtc gccggcggcg tacatgcccg gcatctccgc cgtctctccg
     1261 cccaccaggg cgcagccggc ctggccgcag cctgccgcga tgccggcgat cacctgccgc
     1321 gccgattcga ccgaaagctt gccggtggcg aaatagtcca ggaagaacag cggttcggcg
     1381 ccctgcacca cgaggtcgtt gacgcacatc gccacgaggt cgatgcccac cgtgtcatgg
     1441 atgccggtct cgatcgccac cttcagcttg gtgccgacgc cgtcggtggt ggacaccagc
     1501 accggatcgc ggaacccggc cgccttcagg tcgaacagcg cgccgaagcc ccccagcccg
     1561 cccatcaccc cggcgcggga ggtggcgcgc gcgagcggct tgatcgcttc gaccagcgct
     1621 tcgccggcat cgatatcaac cccggcggcg cggtaggtct gccggttgcg cccggccgcg
     1681 tcgggccgat ctatactggt catggctggc cccgatacgc taaggcggag gatcgtggcg
     1741 gacacaacca taggccacgg ccggagcgca atgcagggcg gcacacggga tgcctgacgg
     1801 atcgcaactc agccggccag cctccgccgc cctggtgaca tggccgaacg tcatcacctt
     1861 cggccggctc tgcgcggtac cgctggcgtt ctggctgatc ctcgaccaca gcatgggcct
     1921 ggccttcttc ctgttcgtcg ccgccggtgc ctcggatgcg gtggatggct ggctggcccg
     1981 ccgcaatggc ggcggcaatt cggtgggcgc cgtgctggac ccggtggccg acaaggcgct
     2041 gctggtgacg atgtatgtga ccctcgcctc ggtgaatgaa ttgccggact ggatcgccat
     2101 attggtggtg ttccgcgatc tggtgatcgt cggcggcgtg ctggtgctgg cggtgctggg
     2161 cgacccggtc accatccggc cgctgtacat ttcgaagctg aataccgcga tgcagttgat
     2221 cctggtggca atgacgctgg ctttggccgg gttcggcctg cacgccgagc tggccaagct
     2281 gctgctgatc tggatcgtcg cagccaccac gctgagctcc ggcgcggcct atgtctgggt
     2341 cacggtgcgc gggcgatgag cgacatcccc accctcgcgc cggatctgga ggtgccaccg
     2401 cccgagcggc cacccgccgc gggaagcccc ggtccgcttc cgtccgtggc gcgccgcagc
     2461 cccgccaccc gggcgcagcg catcgcgctg atcgccggcc tgctggtggt cacctggctg
     2521 gcgctggacc tgttcgcctc cgtgctcgcc cccttcgtgg ccgcggcggt gatcgcctat
     2581 gcgctggacc cgccgaccac ccggctgacc cgcctgggcg tgccgcgcgg catcgccgcg
     2641 ctgctgatga tcgtggctgt gctcgccgcg gtgctgctgt tcctgctgct gctctacccg
     2701 ctgatcatcg cccagcttgg cctgttcctc ggccgcatcc cggtctacgt cggcgagctg
     2761 caggcatggg cgcgggacac catcacccat ctgcaggaga atctcggccc ggacgcggtg
     2821 aacgacaagc tgcgcgacct ggtcgccagc caggccggca acatgctcac cttcctggtc
     2881 tccgcgatca ccagcctgat cggcggcggc ttcgccatct tcaacgtcct gacactggct
     2941 gtggtgacgc cggtggtcgg cttctacctg ctgcgcgact ggcaatgggc ggtgcgccag
     3001 gtggattcct ggctgccgcg ccgctacgcc ggcgtgatcc gcgcccaggc gcgcgaggtt
     3061 gaccgcatcc tgtcggcctg ggtgcgcggc caggcgctgt gctgcgtgct gctggcgctg
     3121 tactacacga tggccttcac cctggcaggg ctggacctgg ggctgatcgt gggcctcgcg
     3181 gcgggcctgc tgtccttcat cccctatgtc ggctcgatca ccggcgggct cactgcgatc
     3241 gccctggcga ccgcgcagtt cccgcattgg cgcggcgtga tcgggattgt cgtcgtgctg
     3301 gtcatcggcc agatcctgga aggctacgtg atctacccgc gcttcctggg cgaccgggtg
     3361 gagctgccgg cggtgtgggt gatcttcgcg ctgttcgccg gcggcgcggc gttcggtttc
     3421 ctgggcgtga tgctggcggt cccggttgcc gccacgatcg gcgtgctgtg ccggttctgg
     3481 ctgcggcgct acctgggcag ccagctctat ctggatccgc cagtccggtc ctgaggcggc
     3541 ggcccgcagt gtcacgcact tgccacgtgc ggccggcgtg actccgcagc acgccgcggg
     3601 ctaacagggc gcctccgcaa ccaccggcag gccgcatgtc cgccacgatc gagccttcgc
     3661 tggcgaccgc cagacgcacg ccgaacctgt gggacctggc ggcgatgctg tgcgtgttcg
     3721 gcaccctggc cgcggtggcg catgtggcac agggcaccat ggtgccgatc aacgcccctg
     3781 gcgcgaccac gatcagcctg gatccgcgca acctgccgga ctacgcggtg cgcaccacgc
     3841 tgcgcatgtt cgccgcgctg gccgcctccc tgctgttcac cttcgcctac ggcaccgccg
     3901 cggcgaagag ccggcgggcc gggctcgtgc tggtgccgct gctggatatc ctgcagtcgg
     3961 taccgatcct gggcttcctc agcttcaccg tcgtgttctt catgcggctg ttccccggcc
     4021 aggtgttcgg cctggaactg gcggcgatct tcgcgatctt caccagccag gcctggaaca
     4081 tggcgttcag cctgtaccag tcgctgcaga cggtgccgcc cgacctgacg gaggccagcc
     4141 gcgccttcca gttgacgccg tggcagcggt tctggcggct ggaagtgccc ttcgcggtgc
     4201 cgggcttggt gtggaacacc atgatctcga tgtccggcgg ctggttcttc gtggtggcat
     4261 cggaagcggt gtcggtcggc aacagcaact ggaagctgcc cggcatcggc tcctatgtcg
     4321 cggtggcgct ggagcagcgc aacatcgccg ccgtgggctg ggcgatcttc gccatgctga
     4381 tcgtcatcct gctgtacgac cagctgctgt tccgtccgct ggtcgcgtgg tcggccaagt
     4441 tccgcgtgga gacgaccgcc ggcgcgacgg cggaggatcc gtggatgctg cgcctgctgc
     4501 gccgcacccg ctggatcgcg gtggcgggcc gccggatggg cgatgcggtg gcgatgctgg
     4561 gcatgctgcg cctgtccttc cgccgccagg cccgccctgc cgcggccggc cgggcgccgt
     4621 cccggctggt ggatgtgctg ctgctggcgg ggctgttcct ggtgctgatg tgggcggccg
     4681 gccggctgag cgccttcgtc gccggcgagc tcgcctggag tgatcttggc cgagcgctgc
     4741 tgcttggcgc ctacacgctg atccgcgtgc tggcgctggt cacgctggcc acggcgatct
     4801 gggtgccggc cggcgtgtgg atcggcctgc gtccggtctg ggctcggcgg gcacagccgg
     4861 tggcccagtt cctggccgcc ttcccggcca acctgctgtt cccgcccttc gtgctgttca
     4921 tcgtgcattt ccggctggat ccggacatct ggctggcgcc gctgatgatc ctgggcaccc
     4981 aatggtacat cctgttcaac gtggtcgccg ccgcggcctc cttccctggc gacctgcggg
     5041 aggcagccag caatttccgc atccacggcc tgctgtggtg gcgcaaggtg atcatccccg
     5101 gcatcctggg cagctatgtc accggcgcgc tggctgccgc gggcggcgcc tggaacgcct
     5161 cgatcgttgc cgaggcggcg tcttggggga acaccacgct gtatgcccat gggctgggcg
     5221 cctacatcgc ccaggccacc ctgcacggcg acatgcagcg ggttgtcctc ggggttgcgg
     5281 tgatggcgct gtacgtggtg acgctgaacc gcgtgctgtg gcgcccgctc tacgcctatg
     5341 cgcagcgccg cctgacactg agctgaccgc cgggaggccg catggacacc accgcgaccg
     5401 cgacgctgct ggaggtgcag aacatccggc agtcctacca caaggacgcc aacgccgacc
     5461 tggtggtgct ggacggcgtg gacctgaccc tgcgcgagcg ggagatggtg gccctgctgg
     5521 gacgctccgg ctcgggcaag tccaccctgc tgcgcatcgt gtccggcctg ctgccgccca
     5581 cccggggcga cgtgctgtgg cgcggcaaga agctgctcgg cccggccgac ggcgtcgcca
     5641 tggtgttcca gagcttcgcg ctgttcccct ggctgaccgt gcaggagaat gtcgaactcg
     5701 gtctggaagc ccgcggcgtg ccgcgcgacg aacgggagcg ccgcgcggaa gcggcgatcg
     5761 acctgatcgg gctgggcggg ttcgagagcg cctatccgaa ggagctgtcc ggcggcatgc
     5821 gccagcgcgt cggcctggca cgcgcgctgg tggtgcatcc cgacctgctg ttgatggacg
     5881 agcccttcag cgcgctggac gtgctgaccg ccgaaacgct ccgcaccgac ctgatcgacc
     5941 tgtggtccga gggacgcctg cccgtgaagt cggtgctgat ggtgacgcac aacatcgagg
     6001 aggcggtgct gatgtgcgac cgcatcctgg tgttctcctc cagccccggc cgcgtggcgc
     6061 acgaactgaa ggtgccgttc ccgcatccgc gcaaccggct ggatccggca ttccgcgaaa
     6121 tggtggacga catctac
//
LOCUS       DASSZH010000663         2903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1618509, whole genome shotgun sequence.
ACCESSION   DASSZH010000663 DASSZH010000000
VERSION     DASSZH010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618509"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..899
                     /locus_tag="VFN42_14330"
     CDS             105..899
                     /locus_tag="VFN42_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HET8997842.1"
                     /translation="MTAIYEVFAIRYATRAAHRSDHFIGGDPHDGPMPMDYFVWVAKN
                     AERTVVIDIGFTAESGGKRGRSLLRDPIDSLALVGVDAAQVRDVILTHMHYDHVGNFH
                     KFPVARFHLQEPEIHYAVGRHMRHRQLAKSFEPDDVCGIVRLNFDGRVTYHNGPADIA
                     PGICVVPTGGHSAGLQFVKVNTARGVVVLASDVTHFYENMESGRPFTTAFHIGDMLDA
                     FDTLRAHAPSPQHIVPGHDPEVMRRYPPPKPELEGVVVRLDVPPSA"
     gene            complement(911..1225)
                     /locus_tag="VFN42_14335"
     CDS             complement(911..1225)
                     /locus_tag="VFN42_14335"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HET8997843.1"
                     /translation="MQITSVTALFSDLGEAELVAWVERGWVLPEQADTGWVFHEIDVA
                     RVRLVHDLRRQMDIGEDSMSLVLSLLDQVYELRARLQAVLRAVEAQPEAVRAAIRAAV
                     LK"
     gene            complement(1227..2138)
                     /locus_tag="VFN42_14340"
     CDS             complement(1227..2138)
                     /locus_tag="VFN42_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HET8997844.1"
                     /translation="MRDPYDVLGVQKTDSESAIRTAYRKLAKKHHPDLNPGKPEAAET
                     FKEIAAAYDILSDADKRARYDRGEIDAAGNEVPPQRPFYRDFSESAGREKYQADISPE
                     DLDSIFAHAFGDGAFFRAGGGRGQQFKARGADAHYTLTVGFLDAANGTTRRITLPEGR
                     TLDVRIPAGVQDGHMLRLKGQGMPGLGGGPAGDALVEIAVAPHPLFHREGDDIIIELP
                     VTLQEAVLGAALEVPTIKGKVRLNIPPNSGTGTRLRLRGRGIRDGHQFVQLHVVLPPS
                     EEPELADFLKSWKPHHAFNPRAGLENA"
     gene            complement(2188..>2903)
                     /locus_tag="VFN42_14345"
     CDS             complement(2188..>2903)
                     /locus_tag="VFN42_14345"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET8997845.1"
                     /translation="AARNALSMALMQALDAELAAIAADPSVRVVVIAGAGPAFCAGHD
                     LREIRATPQREEYQALFAQCSRLMLRLVHLPKPVIARVHGVASAAGCQLVASCDLAVA
                     AGSARFATPGVNIGLFCSTPMVALSRAVGRKQAMEMLLTGELIDAATAQRFGLVNRVV
                     PDAELDAAVAALAEQIAGKSPLTLAIGKEAFYRQAELELEQAYAYASEVMVRNMLARD
                     AEEGMDAFLQKRPPVWTGT"
BASE COUNT          454 a          983 c          975 g          491 t
ORIGIN      
        1 ttcgccttcg gcgacatcac ggcggatgag gcgctgcatt ccatcgacct gttcgcccgg
       61 caggtgatgc cggacctgca tgaagcccgc gtggggattg ccgcatgacc gcgatctacg
      121 aagtcttcgc catccgctac gcgacgcgtg cggcgcaccg gtcagaccac ttcatcggcg
      181 gcgatccgca tgacgggccg atgccgatgg actacttcgt ctgggtggcg aagaacgccg
      241 agcgcaccgt ggtgatcgat atcggcttca ccgcggaaag cggcggcaag cgcgggcgct
      301 cgctgctgcg cgacccgatc gactcgctgg cgctggtggg ggtggatgcg gcgcaggtgc
      361 gggacgtgat cctgacgcac atgcactacg accacgtcgg caatttccac aaattccccg
      421 tggcgcggtt tcatctgcag gagccggaga tccactacgc ggtcggccgc cacatgcggc
      481 acaggcagtt ggcgaagtcg ttcgagccgg atgacgtatg cggcatcgtg cggctgaact
      541 tcgatgggcg tgtaacctac cataacggtc cggcggacat cgcgccgggt atctgcgtgg
      601 tgccgaccgg cggccattcg gcgggactgc agttcgtcaa ggtgaacacc gcgcgcggcg
      661 tcgtggtgct ggcttcggat gtgacgcatt tctacgagaa catggaatcc ggccgacctt
      721 tcaccactgc cttccacatt ggcgacatgc tggacgcgtt cgacacgtta cgcgcacacg
      781 cgccgagccc gcagcatatc gtgccgggcc acgatccgga ggtgatgcgc cgctatccgc
      841 cgccgaagcc ggagctggaa ggcgtggtgg ttcggctgga cgtgccgccc agcgcctgag
      901 tgtgcgggcc ttacttcagc accgccgcac ggatcgcggc gcgcaccgcc tccggctgcg
      961 cttccaccgc gcgcagcacg gcctgcaggc gtgcgcgcag ctcatagacc tggtccagca
     1021 gcgacagcac cagcgacatg ctgtcctcgc cgatatccat ctgccggcgc aggtcgtgaa
     1081 ccagccgcac gcgggcgacg tcgatttcgt ggaacaccca gccggtgtcc gcctgttccg
     1141 gcaggaccca gccgcgttct acccaggcga ccagctccgc ttctcccagg tcgctgaaca
     1201 gcgcggtaac cgaggtaatc tgcatctcag gcgttctcca gtccagcccg cggattgaac
     1261 gcatgatgcg gcttccagct cttcaggaag tccgcgagct cgggttcctc ggagggtggc
     1321 agcaccacat ggagctgtac gaactggtgg ccgtcgcgga tcccgcggcc gcgcagccgc
     1381 aggcgggtgc cggtgccgga gttcggcggg atattcagcc ggaccttgcc tttgatggtg
     1441 ggcacctcca gcgcggcgcc gagcaccgct tcctgcaggg tgacgggtag ttcgatgatg
     1501 atgtcgtcgc cttcacggtg gaacagcgga tgcggcgcga ccgcgatttc caccagcgcg
     1561 tcgccggccg ggccgccgcc caggccaggc atgccctggc ctttcagccg cagcatatgg
     1621 ccgtcctgca cgccggccgg gatgcgcacg tccagcgtgc ggccttccgg cagggtgatc
     1681 cggcgggtcg tcccgttggc ggcgtccagg aagccgacgg tcagcgtgta gtgcgcatcg
     1741 gcaccgcggg ccttgaactg ctgtccgcgt ccgcccccgg cacggaagaa cgcgccgtcg
     1801 ccgaaagcat gcgcgaaaat gctgtccagg tcctccgggc tgatgtccgc ctggtacttc
     1861 tcgcgccccg cgctttcgct gaagtcgcgg tagaacggac gctgcggcgg cacctcgttg
     1921 ccggcggcat cgatctcgcc gcggtcgtag cgtgcacgct tgtcggcatc cgacaggatg
     1981 tcgtacgcgg cggcgatctc cttgaacgtc tcggcggctt cgggcttgcc ggggttcagg
     2041 tcggggtggt gcttcttggc cagcttgcga taggcggtgc ggatggccga ctcgctgtcg
     2101 gtcttctgca cgcccagcac gtcatagggg tctctcataa ggttgcaatc cggttttgcc
     2161 ttaatgaaag aaccggacgg ccgtgtatca agtgccggtc cacaccggag gacgcttctg
     2221 cagaaacgcg tccatccctt cctccgcatc gcgcgccagc atgttgcgca ccatcacctc
     2281 cgaggcatag gcataagcct gttccagttc cagttccgcc tggcgataga acgcctcctt
     2341 gccgatcgcc agggtcagcg ggctcttgcc cgcgatctgc tccgccagcg ccgcgaccgc
     2401 ggcgtccagt tcggcgtccg gcaccacacg attgaccaag ccaaagcgct gcgccgttgc
     2461 ggcatcgatc agctcgccgg tcagcagcat ctccatcgcc tgcttgcgcc cgacggcgcg
     2521 ggacagcgcc accatgggcg tggagcagaa caggccgata ttcacccccg gcgtggcgaa
     2581 gcgggcgctg ccggccgcca cggcgaggtc acagctggcc accagttggc agccagcggc
     2641 ggaggcgaca ccatgtaccc gggcgatcac cggtttgggc aggtggacga ggcgcagcat
     2701 cagccgcgag cactgggcga acagcgcctg gtactcctcg cgctgcggcg tggcgcggat
     2761 ttcgcgtaga tcgtgcccgg cgcagaaggc ggggccggcg ccggcaatca ccaccacgcg
     2821 caccgacggg tccgcggcaa tcgccgccag ctcggcgtcc agcgcctgca tgagcgccat
     2881 cgacagtgcg ttgcgggcgg ccg
//
LOCUS       DASSZH010000664         2415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1550262, whole genome shotgun sequence.
ACCESSION   DASSZH010000664 DASSZH010000000
VERSION     DASSZH010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550262"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VFN42_14350"
     CDS             <1..372
                     /locus_tag="VFN42_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HET8997846.1"
                     /translation="NARRIVFAGLLTAGADLAVDEGRLVIRREGKVRKLTTALDQITF
                     SGRRAASRGQRATYVTERCVLELRPDGLAVTEIAPGVELERDVLAQAEFPLLVPQPPR
                     RMAAALFRAGPIGLHLQGTPA"
     gene            369..1130
                     /locus_tag="VFN42_14355"
     CDS             369..1130
                     /locus_tag="VFN42_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET8997847.1"
                     /translation="MSVALEIADGTALIRLDRPEKLNALDAGMIAALSDAAARIDATA
                     GLRMAILTGSGRAFCVGGDVAAWSALDPLAMGQDWVREGHRVFDRLARLRVPLVAVLN
                     GPALGGGLELAGVADLRIAEPNATLGLPETGIGMVPGWSGTQRLVRRFGAGPVRRMTL
                     FGERFAPEIALRLGLVDEVVDAGEGLARARTLAQGAAGRGRVASILAKQLINAAEGEE
                     GGAVLEMLAGSLVSTTDELKEGVAAFRERRTPRYG"
     gene            1182..>2415
                     /locus_tag="VFN42_14360"
     CDS             1182..>2415
                     /locus_tag="VFN42_14360"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8997848.1"
                     /translation="MRKARLLVGAALAMLLAAGSARAQKPTVEVLHWWTSGGEAAGLS
                     VLRDMVEKQGVTWKDNPIAGGGGGSAMTTLRARYTAGSPPDASQMLGFAIQDWADQGA
                     LADLTPIATKEGWDQAIPKPLWRFSKYEGKWIAAPVNFHSTNWIWVNKADMDKLGAQP
                     PKNFAELIALFDKAKAAGIVPLAQGGQAWQEATMFDSIVLTEGGPDFYKKAMHDLDPA
                     ALSGPIMLKAFEDMATLRKYVDPNFSGRDWNLATAMVIKGDALMQVMGDWAKGEFVAA
                     HKVAGKDFECFRFPGTQGTVTFNSDQFAFFKKGKGLTKGQETMAGDLENKSFQSAFNV
                     IKGSVPARTDVPDTAFDACGKKGMADLKEANAKGTLLGSAAQGYGVPPAVQNAMYDII
                     DRHFNGQIKTEDAPKQLAA"
BASE COUNT          400 a          785 c          894 g          336 t
ORIGIN      
        1 aacgcgcgcc gcatcgtgtt cgccggcctg ctcaccgccg gcgccgatct ggcggtcgat
       61 gagggcaggc tcgtcatccg gcgcgagggc aaggtgcgca agctgaccac cgccctcgac
      121 cagatcacgt tctccggccg ccgcgccgcg tcgcgcggcc agcgcgccac ctatgtcacc
      181 gaacgctgcg tgctggaact gcgccccgac gggctggcgg tgaccgagat cgcgccgggc
      241 gtcgagctgg aacgcgacgt gctggcgcag gcggagtttc cattgctggt gccgcaaccg
      301 ccccggcgca tggcggcggc gctgttccgg gccggtccca tcggcctgca cctgcaaggc
      361 acgccggcat gagcgtcgcg ctggagatcg ccgacggcac cgcgctgatc cggctggatc
      421 ggccggagaa gctgaacgcg ctcgacgccg gcatgatcgc cgcgttgtcc gacgccgccg
      481 cgcggatcga cgcaacggca ggattgcgca tggcgatcct gaccggcagc ggccgcgcct
      541 tctgcgtcgg cggcgacgtg gcggcatgga gcgcgctcga cccgctcgcc atggggcagg
      601 actgggtgcg cgaggggcat cgcgtgttcg accgcctcgc ccgcctgcgc gtgccgctgg
      661 tggcggtgtt gaacggcccc gcgcttggcg gcgggctgga actggccggg gttgcggact
      721 tgcgcatcgc cgaaccgaac gcaactctcg gtctgccgga aaccggcatc ggcatggtgc
      781 cgggctggtc cggcacgcag cgcctggtgc gccgcttcgg cgccggcccg gtgcggcgga
      841 tgaccctgtt cggcgagcga ttcgcgccgg agattgcact gcggctggga ctggtcgatg
      901 aggtggtcga tgcgggcgag ggtctggcgc gggcacgcac gctggcgcag ggcgcggcgg
      961 gtcgcgggcg ggtggccagc atcctcgcga agcagttgat caacgcggcg gagggtgagg
     1021 aaggcggcgc ggtgctggag atgctggccg gctcgctggt ttccaccacc gacgagttga
     1081 aagagggcgt ggcggcgttt cgcgaacggc gaacgccgcg gtatggatga aaatgcgccg
     1141 cgcccgaacg ggtcgggcgc gcgatgtggg gagagagacg gatgcggaag gcaagacttc
     1201 tggtcggtgc cgcactggcg atgctgctgg cggccggttc ggcgcgggca cagaaaccga
     1261 cggtggaagt gctgcactgg tggacctccg gcggcgaggc ggccggcctg agcgtgctgc
     1321 gcgacatggt ggaaaagcag ggcgtgacct ggaaggacaa cccgatcgcc ggcggcggcg
     1381 gcggttccgc catgacgacg ctgcgcgccc gctacaccgc cggcagcccg cccgacgcct
     1441 cgcagatgct cggcttcgcc atccaggact gggcggacca gggcgcactc gccgacctga
     1501 cgccgatcgc gacgaaggaa ggctgggacc aggcgattcc gaagccgctg tggcgctttt
     1561 ccaagtacga gggcaagtgg atcgcggccc cggtcaattt ccactcgacc aactggatct
     1621 gggtcaacaa ggccgacatg gacaagctcg gcgcccagcc gccgaagaac ttcgcggaat
     1681 tgatcgccct gttcgacaag gccaaggccg ccggcatcgt cccgctcgcg cagggcgggc
     1741 aggcgtggca ggaagcaacg atgttcgaca gcatcgtgct gaccgagggc gggccggact
     1801 tctacaagaa agcgatgcat gacctcgacc cggcggcgct gtccggcccg atcatgctga
     1861 aggcgttcga ggacatggcg acgctgcgga agtatgtcga tccgaacttc tccggccgcg
     1921 actggaacct tgccaccgcc atggtgatca agggcgacgc gctgatgcag gtgatgggcg
     1981 actgggcgaa gggcgaattc gtcgcagccc acaaggtggc cggcaaggac ttcgagtgct
     2041 tccgcttccc cggcacgcag ggcacggtga cgttcaactc cgaccagttc gcgttcttca
     2101 agaagggcaa ggggctgacc aagggccagg agacgatggc cggcgacctg gagaacaaga
     2161 gcttccagtc cgccttcaac gtcatcaagg gctccgtgcc cgcgcgcacc gacgtgccgg
     2221 acacggcatt cgacgcctgc ggcaagaagg gcatggccga tctgaaggaa gcgaacgcca
     2281 agggcacgct gctcggctcc gccgcccaag gctacggcgt gccgccggcc gtgcagaacg
     2341 cgatgtatga catcatcgac cggcacttca acggccagat caagacggag gacgcaccga
     2401 agcagctcgc cgccg
//
LOCUS       DASSZH010000665         2464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_169629, whole genome shotgun sequence.
ACCESSION   DASSZH010000665 DASSZH010000000
VERSION     DASSZH010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169629"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="VFN42_14365"
     CDS             complement(<1..1075)
                     /locus_tag="VFN42_14365"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003624095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET8997849.1"
                     /translation="MNVTTRPAMLDVTRIRQDFPILAQKIRGKDLVFLDSAASAQKPR
                     AVIDAMRNAMETQYANVHRGLHWMSERTTDAYEAARDAVAALMNARDRSEVVFVRNST
                     EALNLVAYSYGRHLLQPGQAVLISHMEHHSNIVPWQILRDERGIELRVAPITDAGELD
                     MAAFEHLLSDGKVGLVSITHMSNVLGTVTPAERIVRIAHAHGAKVMFDGSQAVVHRRI
                     DVQALDCDFYAWTGHKLYGPTGIGVLWARRELLDRMPPFLGGGEMISSVTYEKSTWAE
                     VPHKFEAGTPAIIEGIGLKAAIDYVQGIGYDAIAAHEAALTEHALARLSAVPGLRILG
                     QAQDRGGVVSFALDKAHAHDVATL"
     gene            complement(1072..2337)
                     /gene="sufD"
                     /locus_tag="VFN42_14370"
     CDS             complement(1072..2337)
                     /gene="sufD"
                     /locus_tag="VFN42_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET8997850.1"
                     /translation="MTAPVHTGVEGFLARYHGLRDRLPGNLSVRDAAAEAFRRAGLPG
                     RRVEAWKYTDLRPVAAASFAEPLTPLAADSSWLARIPHLDAPRLVFVDGRYRPELSAP
                     PVAAHFDSFASHSDFGPLAQPAREPLVALNTMLAEDGAELRVPDGMDAGALHLVSLAT
                     DSTGESTAFHPRHRIHLEPGARLTLLETSLGDGAYLHNPVTEVLVGKGATLTHVRLQD
                     ESSAAFHLATLYARIGAAGIYDSFVLNLGGRMVRTEVHAHLAGPNAIAHLNGAQLLAG
                     TQHADFTSVVRHDAPNCNSRQTVKNVLAGRSRGVFQGRIEVARHAQKTDGFQMNQALL
                     LSPDAEVDTKPELEIFADDVKCSHGATVGELDAEQLFYLRSRGVPDSEARSILVRAFL
                     AEALQAVAPDDIRSILEDSVERWWQRQPA"
BASE COUNT          385 a          845 c          826 g          408 t
ORIGIN      
        1 gcagcgtcgc cacgtcgtgc gcatgcgcct tgtccagcgc gaacgacacc acgccgccgc
       61 gatcctgcgc ctggccgaga atccgcaatc ccggcaccgc ggagagccgc gccagcgcgt
      121 gctcggtcag tgccgcctcg tgcgcggcga tggcgtcgta gccgatgccc tgcacgtaat
      181 cgattgccgc cttcaggccg atgccttcga tgatcgccgg cgtgccggcc tcgaatttgt
      241 gcggcacctc ggcccaggtg cttttttcgt aggtcaccga cgagatcatc tcgccgccgc
      301 ccaggaacgg cggcatgcgg tccagcagct cccgccgcgc ccacagcacg ccgataccag
      361 tgggaccata cagcttgtgt ccggtccagg cgtagaagtc gcaatccagc gcctgcacgt
      421 cgatccgacg gtgcaccacg gcctgggagc catcgaacat caccttcgcc ccgtgcgcgt
      481 gcgcgatgcg tacgatccgc tccgccgggg tgaccgtgcc cagcacgttg gacatatgcg
      541 tgatggagac caggccgacc ttgccgtcgg acagcaggtg ctcgaatgcc gccatgtcca
      601 gttcgccggc atcggtgatc ggcgccacgc gcagttcgat gccgcgctca tcccgcagaa
      661 tctgccaggg cacgatgttc gagtggtgct ccatatggga gatcagcacc gcctggccgg
      721 gctgcagcag gtgccggccg tagctgtagg cgaccagatt cagcgcctcg gtgctgttgc
      781 gcacgaacac cacttccgaa cggtcgcgcg cattcatcag cgccgccacc gcgtcgcggg
      841 cggcttcata cgcatcggtg gtgcgctcgc tcatccagtg caggccgcga tgcacgttgg
      901 cgtattgcgt ctccatcgca ttgcgcatgg cgtcgatgac ggcacgcggc ttctgcgccg
      961 aggccgcgct gtccaggaac accaaatcct tgccgcggat cttctgcgcc aggatgggga
     1021 agtcctgccg gatccgcgtg acgtccagca tggcgggtcg ggtcgtgacg ttcatgcagg
     1081 ctgcctctgc caccagcgct cgacgctgtc ttccagtatg gaacggatat cgtccggcgc
     1141 caccgcttgc agcgcctccg ccaggaaggc gcggaccagg atcgagcggg cctcgctgtc
     1201 cgggacgccc cggctgcgga ggtaaaatag ctgctccgca tccaactcgc cgaccgtggc
     1261 gccgtggctg cacttcacgt cgtccgcgaa gatctccagc tccggcttgg tatcaacctc
     1321 ggcatccggc gacagcagca gcgcctggtt catctggaac ccgtcggtct tctgcgcgtg
     1381 gcgggcgact tcgatgcggc cctggaacac gccgcgcgag cggccggcca gcacgttctt
     1441 caccgtctgc cgggaattgc agtttggcgc gtcgtggcga accacgctgg tgaagtcggc
     1501 atgctgcgtg ccggccagca gttgcgcccc gttcagatgc gcgatggcat tcgggccggc
     1561 gaggtgggca tgcacctcgg tgcgcaccat gcggccaccc aggttcagca caaagctgtc
     1621 atagatgccg gctgcaccga tccgcgcgta gagcgtagcg aggtggaacg ccgcgctgga
     1681 ttcgtcctgc agccgcacat gcgtcagcgt ggcacccttg ccgaccagca cttcggtgac
     1741 cgggttgtgc aggtaggcgc catcgccaag gctggtttcc agcagcgtga gcctggcgcc
     1801 gggttccaga tgaatccgat gccgcgggtg gaatgccgtg ctctcgccgg tgctgtcggt
     1861 ggccaggctg accaggtgca acgcaccggc atccatcccg tccggcaccc gcaactcggc
     1921 gccatcctcc gccagcatgg tgttgagtgc caccagcggc tcgcgtgccg gctgcgccag
     1981 cgggccgaag tcgctatgcg aggcgaagct gtcgaagtgt gcggcaaccg gcggcgcgga
     2041 cagctccggc cggtagcggc cgtcaacgaa taccagacgc ggcgcatcca aatgcgggat
     2101 acgcgccagc caggaactgt cggcggcgag cggggtcagc ggttcggcga acgaagcggc
     2161 ggcgacgggg cgcagatcgg tgtatttcca cgcctccacc cggcgaccgg gcaggccggc
     2221 acggcgaaac gcctccgcgg cggcgtcgcg cacgctcagg ttgccgggca agcggtcgcg
     2281 caggccgtgg tagcgggcga gaaaaccttc gacgccggtg tgtaccgggg cggtcatgca
     2341 gacgccttcc tgtcgtggcc gaacatctgc gtctcgtggc cgaacatctc cgtgtcatgg
     2401 ccgggcttgg cccggccacc cacgtctatg gtcaactcac ccagcaagtc gtggggggcc
     2461 gggc
//
LOCUS       DASSZH010000666         4250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_664236, whole genome shotgun sequence.
ACCESSION   DASSZH010000666 DASSZH010000000
VERSION     DASSZH010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664236"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..2552)
                     /locus_tag="VFN42_14375"
     CDS             complement(222..2552)
                     /locus_tag="VFN42_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET8997851.1"
                     /translation="MTATKFGIAQPVRRVEDPRLLKGAGRYTDDIALPGMLAGVVLRS
                     PHAAARLGAIDTSEAAAMPGVRAIYTAADLKADGVQPMPCAAPLQNRDGSDMALPPHP
                     ALAEGAVRHIGDPVAFIVADSAKQARDAAEAILVAYDIQPSITELPRAMEAGVPQVWP
                     QVKNNLAFDWEIGDKAATEAAFAKAAHVTKLTVVNNRIIVSSMEARVAIADYDSSTQR
                     WVLYANTQGGWLVKGLIAPVFGVAPDQFRVVTPDVGGGFGMKLFLYAEHVLTCYAARK
                     LGRPVKWVSERSEAFVADTQGRDNVTTGELAIDSNGKFLALRTRNVANMGAYLSSFAP
                     YIPTVAGTGVLASVYGFQAAYANVLGVYTNTVPVDAYRGAGRPEANYLVERLVDAAAR
                     ELKIDRAELRRRNMVPPEAMPHSTPMGKSYDSGDFHRVLDTGLGLMDYAGFAARRAEA
                     AKRGKRCGFGFSYYLEATGGDPTERAEIRFADDGFVDVYVGTQSTGQGHETAYVQLTA
                     NRLGIDGEKIRIRQGDTDTIPVGGGTGGARSLYSEGQAIFATTDTVISKGKQAAAEVL
                     EAAVADISFAEGRFSIVGTDRGIDILSLAQTQRKKVAAGQQATTLDAAEVANIDFHTF
                     PNGCHMAEVEIDPDTGVVQVVRYTVCDDVGKAVNPLIVRGQVHGGVAQGTGQALFERT
                     AYDPESGQLLSGSFMDYALPRADDLPNIDVEFVEVPCATNPLGVKGAGEAGAVGSPPA
                     LVNAVLDALAGDGVTHIDMPATPEKVWRALSLAKAA"
     gene            complement(2677..3105)
                     /locus_tag="VFN42_14380"
     CDS             complement(2677..3105)
                     /locus_tag="VFN42_14380"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HET8997852.1"
                     /translation="MTAVVVDASVAVKWVVQEDHSACAVRLLAYQARHAPAHWQAEAV
                     NALWSKVFKRELPAEDAAERASVLLRAPVIGASISELMPLAFSISVAQSVTVYDSLYL
                     ALAEKLDIPLVTADRRLIARMAGSSSAGRLIWVGDVQPAS"
     gene            complement(3102..3353)
                     /locus_tag="VFN42_14385"
     CDS             complement(3102..3353)
                     /locus_tag="VFN42_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997853.1"
                     /translation="MGQLIVRGLDDRLIQSLKQRAARYGRSAEAEHRAILEQALSPEL
                     EAFEQVAARLRARTPRQTSDSADLIRQDRDRDHAGSGQP"
     gene            3491..>4250
                     /locus_tag="VFN42_14390"
     CDS             3491..>4250
                     /locus_tag="VFN42_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997854.1"
                     /translation="MNEHPALEAVADEAVLVETTAQAPESANAAFERHFAAPERNPFF
                     GWNPPPQPIRHHILSEVVLDGRFRGLFKSGRFIPGTGPRPPDQPPAADAARPATTAAD
                     GTAIIGCNPGHAQPFHWLTQSLPAIDVAMRREGQDRRTVLVLPPLTPWQEETLRILGY
                     VETRRIPIDQPDAHYAFARAEFSEYLAGQAVFRLSRHARDIYARLRDSVPVSLSEGRR
                     LYLAGGGPIDNEAALIEALQQRGFEIVTQSGLSLT"
BASE COUNT          670 a         1507 c         1381 g          692 t
ORIGIN      
        1 cccgacacca actttcggcc tccggaacgt tcaagagttt tcgcacagcc tggaaggccg
       61 gccacccacg tcttgattgt ccacggctgc gaaaagacgt gggtggccgg ccttcgccgg
      121 ccatgacacg ggggagtgaa ccggctcacc accgcaactt taacgcctgt ttttaggcct
      181 atggggcgca ggcccgccac gacggcgttt gcttgcgctg actacgccgc cttcgccagc
      241 gacagcgccc gccacacctt ctccggcgtc gccggcatgt cgatatgcgt cacgccgtcg
      301 ccggccagcg catccagcac cgcgttcacc agcgccggcg ggctgccgac cgcgcctgcc
      361 tccccggcgc ctttgacacc cagcgggttc gtcgcgcagg ggacctcgac gaattcgacg
      421 tcgatgttcg gcaggtcgtc ggcacgcggc agcgcgtagt ccatgaaact ccctgacagc
      481 agctggccgg attccggatc gtacgcggtg cgctcgaaca gcgcctggcc ggtgccctgc
      541 gccacgccgc catgcacctg gccgcgcacg atcagcgggt tcaccgcctt gccgacgtcg
      601 tcgcacaccg tgtagcgcac cacctgcacc acgccggtat cgggatcgat ctccacctcc
      661 gccatgtgac agccattggg gaaggtgtgg aaatcgatgt tcgccacttc cgcggcgtcc
      721 agcgtggtgg cctgctgtcc cgccgccacc ttcttccgct gcgtctgcgc cagcgacagg
      781 atgtcgatgc cgcggtcagt gccgacgatg gagaaccgcc cctcggcaaa gctgatatcg
      841 gcaaccgccg cttccagtac ttccgccgcc gcctgcttgc ccttgctgat caccgtgtcg
      901 gtggtggcaa agatcgcctg gccttccgaa tacaggctgc gcgcgccgcc ggtgccgccg
      961 ccgaccggga tcgtatcggt gtcgccctgg cggatgcgga tcttctcgcc gtcgatgccc
     1021 aggcgattgg cggtgagctg cacatacgcc gtctcgtggc cctggccggt ggattgcgtg
     1081 ccgacataga catcgacgaa gccatcatcg gcaaagcgga tctccgctcg ctcggtcgga
     1141 tcgccgccgg tggcctccag gtaataggaa aacccgaagc cgcaccgctt gccgcgcttc
     1201 gccgcttccg cgcgccgcgc cgcgaagccc gcgtagtcca tcagccccag gccggtatcc
     1261 agcacccggt ggaaatcgcc actgtcatag gacttgccca tcggcgtgct gtgcggcatc
     1321 gcctcaggcg gcaccatgtt gcggcggcgc agctccgcgc gatcgatctt caactcgcgc
     1381 gcggcggcat ccaccagccg ttccaccagg tagttcgctt ccggtcgccc ggcgccgcga
     1441 taggcgtcca ccggcaccgt gttggtatag acgcccagca cgttcgcgta ggccgcctgg
     1501 aaaccgtaca cgctggccag cacaccggtg ccggcaaccg tggggatata cggcgcgaag
     1561 ctggacaggt acgcgcccat attggcgacg ttgcgcgtgc gcagcgccag gaattttccg
     1621 ttgctgtcga tcgcgagttc gccggtggtg acgttgtcgc ggccttgcgt atcggcgacg
     1681 aaagcctcgc tgcgctccga cacccatttc accggccggc ccagcttgcg tgccgcgtag
     1741 caggtcagca cgtgttcggc gtagaggaac agcttcatgc cgaacccgcc gcccacatcc
     1801 ggagtgacga cgcggaactg gtccggcgcc acgccgaaca ccggcgcgat caaccctttc
     1861 accagccagc cgccctgggt gttggcatac agcacccagc gctgggtgct gctgtcgtaa
     1921 tcggcgatgg cgacgcgggc ctccatcgag gagacgatga tacggttgtt caccaccgtc
     1981 agcttcgtca catgcgccgc cttggcgaag gcggcctcgg tcgcggcctt gtcgccgatc
     2041 tcccaatcga acgcgaggtt gttcttcacc tgcggccaga cctgcggcac gcccgcttcc
     2101 atcgcccgcg gcagttcggt gatggacggc tggatgtcat acgccaccag gatcgcctca
     2161 gcggcgtcgc gcgcctgttt cgcggaatcg gcgacgatga aggcgaccgg atcgccgata
     2221 tgccgcaccg cgccctcggc cagcgcggga tgcggcggca gcgccatgtc ggagccgtct
     2281 cggttctgca gcggtgcggc gcagggcatc ggctgcacgc cgtccgcctt caggtcggcg
     2341 gcggtgtaga tggcgcgcac gccgggcatg gctgcggcct cactggtatc gatggcgcca
     2401 agcctggcgg cggcgtgtgg gctgcgcagc accaccccgg ccagcatgcc aggcagggcg
     2461 atgtcgtcgg tatagcgccc cgcccctttc agcaggcgag gatcctccac ccggcgtacg
     2521 ggctgggcga tgccgaactt ggtggcggtc atggggcagg gtgtctccac ggctggtctg
     2581 gcaggggggc ggaggatgcc cgccgaaccg ggcaaggcca aggggcggcg gacggtgggt
     2641 cgcgctggcg tgaagagtcc cggcgtcgcg atgcgttcac gaagcgggct ggacgtcgcc
     2701 gacccagatt agtcgcccgg cggaactgct ccccgccatc cgcgcgatca gccgccggtc
     2761 cgccgtcacc aggggaatgt cgagcttttc cgccagggcg aggtacagtg agtcataaac
     2821 cgtcaccgac tgtgcaacgg aaatcgaaaa ggccagaggc atcaactcac tgatcgaagc
     2881 gccgatcacg ggcgcccgca acaacacact ggcccgctcc gcggcatctt cggcaggcaa
     2941 ttcgcgcttg aagaccttcg accaaagcgc attcaccgcc tccgcctgcc agtgcgccgg
     3001 ggcgtggcgc gcttggtacg ccagcagccg taccgcacag gccgaatggt cctcctgaac
     3061 cacccatttc accgcgacgg aggcgtcgac aacgaccgct gtcacggctg accggatccg
     3121 gcatgatccc ggtcgcgatc ttgccggatc aggtcggcac tatcggatgt ctggcgcggt
     3181 gtccgcgcgc gaagccgcgc cgccacttgc tcaaacgctt ccaactcggg agacagggcc
     3241 tgttccagga tcgccctgtg ctctgcctcc gcggatcggc cgtagcgtgc ggcgcgttgc
     3301 ttcagggact ggatcagccg gtcgtccagt ccgcgcacga tcagttggcc catgcctgcc
     3361 tcccggcatg atatcgaatc acgatatcat gccttcatcc gaaccggaag gagccgatcg
     3421 cctgttgcgc cccggccggt ttccgctatc ccgtcccctc ggacggaagg ctgcagtgac
     3481 gaaccttggc atgaacgagc atccggcgct cgaagcggtg gcggacgagg cggtcctcgt
     3541 ggagaccacc gcgcaggccc cggaatccgc gaacgccgcg ttcgagcgac actttgccgc
     3601 gccggagcgc aatccgttct tcggctggaa cccgccgccg cagccgatcc gccaccacat
     3661 cctgtcggag gtggtgctcg acgggcggtt ccgcgggctg ttcaaatccg gccggttcat
     3721 tcccggcacc ggcccccggc cgccggatca gccaccggca gccgatgccg ccaggcctgc
     3781 cacgacggcg gcggacggca cggcgatcat cggctgcaac ccgggccacg cccagccctt
     3841 tcactggctc acccaatccc tgccggccat cgacgtggcg atgcggcggg agggtcagga
     3901 ccggcgcacc gtcctggtcc tgccgccgtt gacgccgtgg caagaggaaa cgctgcgcat
     3961 cctgggctac gtcgagacca ggcggatccc gatcgatcag cccgacgcgc actatgcctt
     4021 cgcccgggcc gagttcagcg aatacctcgc cggacaggcg gtattccggc tgtcccgcca
     4081 cgcgcgcgat atctacgcgc ggctgcgcga cagcgtgccg gtcagcctgt cggaggggcg
     4141 caggctttac ctggccggcg gcggaccgat cgacaacgaa gccgcgctga tcgaggcctt
     4201 gcagcagcgc ggcttcgaga tcgtgacgca gagcgggctg agcctgacgg
//
LOCUS       DASSZH010000667         2596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1362476, whole genome shotgun sequence.
ACCESSION   DASSZH010000667 DASSZH010000000
VERSION     DASSZH010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362476"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="VFN42_14395"
     CDS             <1..757
                     /locus_tag="VFN42_14395"
                     /inference="COORDINATES: protein motif:HMM:NF014640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET8997855.1"
                     /translation="AQKNSRLIGSLNRLTQESRLRLSRVERGIPDLPALLGAARQRLD
                     DRAERAMLALPALVARRRMVLTGIERRLPDPASLTAAARDTMRDRALRLRLSAPGLVA
                     ARRASLEVASHRLTGAVHRALTTLRGRADRVVGRVSEAPLRSALREARAHLAGLGPRL
                     DAASPMAILQRGYVLVTNPAGQPVTSAAAVKPGNRLHLQFGDGLVDAVAQGHPATGAT
                     AHDRGSKDGGHKGSGNQGSSRVRTATAQETLDL"
     gene            complement(752..1600)
                     /locus_tag="VFN42_14400"
     CDS             complement(752..1600)
                     /locus_tag="VFN42_14400"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8997856.1"
                     /translation="MQDLKGKTAIVTGAASGIGLGIAKALASAGVNIVLADLRPEPLE
                     AARKDVEALGARTAAVTVDVSDPDSVAAAAQAATDAFGALHIAVNNAGVAMHGTPVEK
                     VTPQEWDWLIGVNVRGVINGIRSFLPLIRSHGQGGHIVNTGSVSSLFVREGRNQGAYA
                     MSKYAVLALSEALEQELAGSGIGVSILCPVGVRTSIFQSAATRPDRFGGSYSRPEQEA
                     LQSGFFETALDPLVIGRRVLQAIQDNEFYVLTHTGERASITARFDRIRAAFDRADAVM
                     PTLTGS"
     gene            complement(1605..1994)
                     /locus_tag="VFN42_14405"
     CDS             complement(1605..1994)
                     /locus_tag="VFN42_14405"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET8997857.1"
                     /translation="MADDKRAQGQAMRRKLMGEAYSKKVDSKVYNNPIMEKFAAYTQE
                     AVFGTLWTRPGLDLKTRALICVISDAAQGREPELKLHLRFARNQGWTEDELSEALLHL
                     AGYIGAPLVREALLAAVEVFKDMAADE"
     gene            complement(2072..>2596)
                     /gene="ureG"
                     /locus_tag="VFN42_14410"
     CDS             complement(2072..>2596)
                     /gene="ureG"
                     /locus_tag="VFN42_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HET8997858.1"
                     /translation="RAYDVAAITNDIYTKEDAEFLTRAGALDPERIMGVETGGCPHTA
                     IREDASINLAGVAEMQRRFPALDIILIESGGDNLAATFSPELADLTIYVIDVSAGDKI
                     PRKGGPGIMRSDLLVINKIDLAPFVGASLEVMDRDARKMRGDRPFVFANIRAGTGVPE
                     IAAFIEKSGGLRPS"
BASE COUNT          402 a          883 c          882 g          429 t
ORIGIN      
        1 ggcgcagaag aattcccgcc tgatcggcag cctgaaccgg ctgacgcagg aaagccggtt
       61 gcggctgtcg cgcgtcgagc ggggcattcc cgacctgccg gccctgctgg gggcggcgcg
      121 gcagcgcctg gatgaccgag ccgagcgggc gatgctggca ttgccggcgc tggtggcgcg
      181 ccggcggatg gtgctgacgg gcattgaacg ccggcttccc gatcccgcca gcctgaccgc
      241 cgccgccagg gacacgatgc gggaccgcgc cctgcgcctg cgcctgtcag cccctggcct
      301 ggtcgctgct cggcgcgcca gcctggaggt tgcgtcccac cgcctgaccg gcgcggtgca
      361 ccgggcgttg accacgctgc gcggccgtgc ggaccgggtc gtcggccgcg tttcggaagc
      421 cccgctccgc tcggcgctgc gggaggcgcg ggcgcatcta gccgggctgg ggccgcgcct
      481 ggacgcggca tcgccgatgg cgatcctgca gcgcggctat gtactggtaa ccaatccggc
      541 ggggcagcca gtgaccagcg cggccgccgt caagcccggc aaccgcttgc accttcaatt
      601 cggcgatggc ctggtcgatg ccgtcgcaca gggccatccg gcaaccggcg ccaccgccca
      661 tgaccgcggg agcaaggatg gcggtcacaa gggtagcggc aaccagggca gcagccgggt
      721 tcggaccgca accgcccagg aaacgctgga cctatgagcc ggtcagcgtc ggcatcaccg
      781 cgtcggcgcg gtcgaaggcg gcgcgaatcc ggtcgaagcg ggcggtgatg ctggcacgct
      841 ccccggtatg ggtcaggacg tagaactcat tgtcctgaat ggcctggagc acgcggcggc
      901 cgatgaccag gggatcgagc gcggtttcga agaagccgga ttgcagcgcc tcctgctccg
      961 gccggctata gctgccgccg aaacggtccg gccgcgtcgc cgcggactgg aagatcgacg
     1021 tccgcacacc aaccgggcac aggatggaca cgccgatgcc ggacccggca agttcctgct
     1081 ccagcgcctc ggacaaagcc agcacggcat acttgctcat ggcataggcg ccctggttcc
     1141 gcccctcccg cacaaacagg ctggacaccg agccggtatt gacgatgtgg ccgccctgcc
     1201 cgtggctgcg gatcagcggc aggaaactgc ggatgccgtt gatcacaccc cgcacgttga
     1261 cgccgatcag ccagtcccat tcctgcggcg tcactttctc gaccggtgtg ccatgcatcg
     1321 ccaccccggc gttgttgacc gcgatgtgca gcgcgccgaa tgcgtcggtg gcagcctggg
     1381 cggcagcagc gaccgaatcg gggtccgaca catcgaccgt gaccgcggcg gtgcgggcgc
     1441 ccaatgcctc aacgtccttg cgcgccgctt ccagcggttc cggccgcaga tcggccagca
     1501 cgatgttgac gccggcgctg gcgagggcct tggcaatgcc gaggccaatg ccggacgccg
     1561 cgccggtgac gatggcggtc ttgcccttca ggtcctgcat gggatcactc gtccgccgcc
     1621 atgtccttga acacttcaac cgccgccagc agagcctccc gcaccagcgg ggcgccgatg
     1681 tagccggcca ggtggagcag cgcttccgac agttcgtcct cggtccagcc ctggttgcgt
     1741 gcgaagcgaa gatgcagctt caattccggc tcccgcccct gggcggcgtc ggaaatcacg
     1801 cagatcagtg cacgggtttt cagatcgagg ccgggacggg tccacagggt gccgaacacc
     1861 gcttcctggg tgtaggcggc gaatttttcc atgatgggat tgttgtagac cttggagtcg
     1921 accttcttcg aataggcctc gcccatcagt ttgcggcgca ttgcctgccc ctgggcgcgt
     1981 ttgtcgtcag ccatgatgcc tgtttccctc ccgtcgatgg gaggagtttc aggcaaaggg
     2041 ccgaatttgg ctaggggccg gcggcagggg attaggacgg acgcaggccg ccgctctttt
     2101 caatgaaggc ggcgatttcc ggcacaccgg tcccggcgcg aatgttggcg aagacaaacg
     2161 gccgatcgcc gcgcatcttc ctggcatcgc ggtccatcac ctccaggctc gcccccacga
     2221 acggtgcgag gtcgatcttg ttgatcacca gcagatcgct ccgcatgatt cccggtccgc
     2281 ccttccgagg gatcttgtcg ccggccgaca cgtcgatgac gtagatggtc aggtccgcca
     2341 gttccggact gaacgtcgcc gccagattat cgccgccgct ttcgatcagg atgatgtcca
     2401 gtgccgggaa ccgccgctgc atttccgcca ccccggctag gttgatcgat gcgtcctcgc
     2461 ggatcgcggt gtgcggacag ccgcccgtct cgacacccat gatccgctcg gggtcgagcg
     2521 cgccggcccg tgtcaggaac tccgcgtcct ccttggtgta gatgtcgttg gtgatcgcgg
     2581 cgacgtcgta ggcgcg
//
LOCUS       DASSZH010000668         4406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_374373, whole genome shotgun sequence.
ACCESSION   DASSZH010000668 DASSZH010000000
VERSION     DASSZH010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374373"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VFN42_14415"
     CDS             <1..553
                     /locus_tag="VFN42_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008850513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HET8997859.1"
                     /translation="LGGAVLSSTKWINDVLQPFIRNTGPALSAFNAWLLLKGLETLKL
                     RVEAGCRSAAALADFLAEQPGVSRVWYPTRADHPQRALAMTQMTAGGTVVTFEVAGAK
                     DAAFRVMNAFQLIAVSNNLGDSKSLATHPATTTHMRIGADERAKLGITDGVIRLSVGL
                     EDVADLRDDLAQALAPKRAQAAA"
     gene            complement(538..1374)
                     /locus_tag="VFN42_14420"
     CDS             complement(538..1374)
                     /locus_tag="VFN42_14420"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET8997860.1"
                     /translation="MNWETIAAPPGLPPPRPLMVRVQSIPARHAFPEHAHAWNQVVYA
                     VSGVLTVSVEGRSFVISPEQAVWLPTGLRHRVGSLLGAEFRSLWIAAEAGLGLPDTPT
                     VFGVAPLLQWLIVEAASLEGRADPDGYAGRVTRLILDQLRRARPVAGALPWPRGGALM
                     KLCTALYADPSDRRGPEQWGQELAMSGRTLARRFEAELGMSLRSWRRRLRLFKAVELL
                     GGGVSVTQTAMELGYGSTSAFVYAFRTELGRSPQAYMRGRAADRGMATRPAAAMPVYA
                     AA"
     gene            1589..2077
                     /locus_tag="VFN42_14425"
     CDS             1589..2077
                     /locus_tag="VFN42_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HET8997861.1"
                     /translation="MQPGSGSPVRYMQRTRAYYRALGYTKDYVWAHHADVPFIRPAKP
                     ASAMRIALICTAGPGDRSHRDEHNRRHVWSGSVASPPDSFDTDVAWDRESTHTDDRET
                     FLPISAARRLVAEGALGALGPRFHAAPTEYSQQKTTGRDAPDILRRLWEDAVDAAILT
                     AL"
     gene            2141..2467
                     /locus_tag="VFN42_14430"
     CDS             2141..2467
                     /locus_tag="VFN42_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997862.1"
                     /translation="MIIGSALDIVEHCGVPRFVFSDFPLGNPCGHPWNPAMQTETLRL
                     ALQLLETATGPRTTLRSPFAWKAEDPGWRERYNRIRPEDREKLLALGDARRRRFGQAP
                     HSQTPR"
     gene            2649..3878
                     /locus_tag="VFN42_14435"
     CDS             2649..3878
                     /locus_tag="VFN42_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8997863.1"
                     /translation="MSQATLEAAGQAAPPAMHPRGNVLRLSVAQALAGANSTLVYAAA
                     AIVGHTLAPSKLLATLPISVYVVGMAVCTLPAGAVARRYGRRAAFLTGTGCGAIAGLL
                     STLAILLNAFWLFCIGTCFGGAYGAVVLSYRFAAADCVPPERRARALAAVMAGGVFAG
                     VIGPQLVTYTMELWQPYLFAATFLAQAAVAVLSALIVAGVRLPKPSAAEIVGGGRPLG
                     VILREPRFIIAMICGTVAYMLMNFLMTTAPLAMRLCGISQSAANLGLQWHVIAMYAPS
                     FFTGRLIGRFGAPRVVAVGLTLFAAAGVMGLTGTDVTHFWLSLILLGLGWNFSWLGAS
                     ALVLECHRPEERTRVQSLNDFVVFGMMALGSFSAGGLLLSFGWDTVIWVSFLPLAAAV
                     AALGYRAAARSRLFPAA"
     gene            3922..4260
                     /locus_tag="VFN42_14440"
     CDS             3922..4260
                     /locus_tag="VFN42_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997864.1"
                     /translation="MITEIIVFDLPEGMTRDEVVANYRRSAPGWRANPDLIRKNYLYD
                     AEGRRAGGVYLWRDMDAARRARDAAWLERVRRTYGSEPVVQYFATPLLVDNALDRTIE
                     EADARDHAPA"
BASE COUNT          656 a         1552 c         1524 g          674 t
ORIGIN      
        1 gctgggcggc gcggtgctgt ccagcacgaa atggatcaac gacgtgctgc agcctttcat
       61 ccgcaacacc gggccggcgc tgtcggcgtt caatgcctgg ctgctgctga aggggctgga
      121 gacgctgaag ctgcgggtcg aagccggctg ccgcagcgcc gccgcgctgg cggatttcct
      181 ggccgagcag ccgggggtga gccgggtgtg gtatccgacc cgcgcggatc atccgcaacg
      241 cgcgctggcg atgacgcaga tgaccgcggg cggcacggtg gtgacgttcg aggtggccgg
      301 cgccaaggat gcggcgttcc gggtgatgaa cgcgttccag ctgatcgcgg tatccaacaa
      361 cctgggcgat tcgaaatcgc tggcgacgca cccggccacc accacgcata tgcgcatcgg
      421 cgccgacgag cgcgcgaagc tgggcatcac cgatggggtg atccgcctgt cggtcgggct
      481 ggaggatgtg gcggacctgc gcgacgactt ggcccaggcg ctggcgccga agcgggctca
      541 ggcggcggcg taaaccggca tcgctgcggc gggtcgcgtg gccataccgc gatccgccgc
      601 tctgccgcgc atatacgcct ggggactgcg gcccagttcg gtgcggaacg catagacgaa
      661 cgccgaggtc gagccgtagc cgagctccat cgccgtctgc gtcacgctca ccccgccgcc
      721 cagcagctcg accgccttga acagccgcag gcgccgccgc caggagcgca ggctcatgcc
      781 cagctcggcc tcgaagcggc gtgccagggt gcgccccgac atggcgagtt cctgccccca
      841 ttgctccggt ccgcgccggt cggacgggtc ggcgtacagg gcggtgcaca gcttcatcag
      901 cgcgccgccg cgcggccagg gaagcgctcc cgccaccgga cgcgcacgcc ggagctggtc
      961 caggatcagc cgggtcaccc gacccgcata gccgtcggga tcagcgcgcc cctccagtga
     1021 cgcggcttcc acgatcagcc attgcagcag cggcgcgacg ccaaacacgg ttggcgtgtc
     1081 cggcaggccc agaccggctt cggcggcaat ccacaggctg cggaattccg cgccaagcag
     1141 cgagcccacc cggtgccgca gtcccgtggg cagccacacc gcctgctccg gcgagatgac
     1201 gaaggagcgt ccctccaccg agaccgtcag cacgccggag accgcataga cgacctggtt
     1261 ccaggcatgc gcgtgctccg gaaaggcgtg acgcgcgggt atcgactgca cgcgcaccat
     1321 cagcggcctg gggggaggca agccgggcgg tgccgcaatc gtctcccaat tcatcggcga
     1381 tctccgtctc tgtcgggtat tctatatgcg tctttggacc atccaaatac cgccatgacc
     1441 gcacggcgag gccggcgatt gccgatcttg gcagatgagc gatacgatct ggcagattgt
     1501 caaaagccgc cctgcgccgg agttgctaaa gggcaaccgt gacagagacc gcaccgcggc
     1561 gggacgtcgc gcgcgaggga ggactccgat gcagccaggc tccggatcgc cggtccgcta
     1621 catgcagcgc acgcgggcgt attatcgcgc tctgggctac accaaggatt atgtctgggc
     1681 gcatcacgcg gacgtcccgt tcatccgtcc cgccaagccg gccagcgcga tgcggatcgc
     1741 gctgatctgc accgcgggtc cgggcgaccg ctcgcatcgc gacgaacaca accgccggca
     1801 tgtctggagc ggctcggtcg ccagcccgcc ggacagcttc gacaccgatg tggcctggga
     1861 ccgggaatcg acgcacaccg acgaccgcga aaccttcctg ccgatcagcg cggcgcggcg
     1921 cctggtggcg gaaggcgcgc tgggtgccct cggaccgcgc ttccacgccg cgccgactga
     1981 gtacagccag cagaagacga ccggacggga cgcgccggac attctgcgcc ggctctggga
     2041 ggacgcggtc gatgccgcga tcctgaccgc cttgtgaccc gtctgccacc agaccgtgag
     2101 tctggttgcc cgacatctgg aagcgaacgg gattccgacg gtgatcatcg ggtcggctct
     2161 cgatatcgtc gagcattgcg gcgtgccgcg gttcgtgttc tcggatttcc cgctgggcaa
     2221 cccgtgcggc catccctgga acccggcgat gcaaacggag acgctgcggc tggccctgca
     2281 actgctggag acagccaccg ggccgcgcac cacgctgcgg tcgcccttcg cctggaaggc
     2341 ggaggatccc ggctggcgcg aacgctacaa ccgcatccgg cccgaggatc gggagaagct
     2401 gctcgccctg ggcgatgccc gccgccggcg cttcgggcaa gcgccgcact cgcagacgcc
     2461 gcgataggaa cacgcggggc cccctgcgcc acgcgctacc agcttccggt ttggcggtta
     2521 ttccatgcga tctgtctgga cgtcgaaaga cagccagagg cggatggact atctccggcc
     2581 gcgcagccgg cagcgtgcca gcccgccggc cgcgccggtg accaccgaca ttccgcctga
     2641 ggactccaat gtcgcaagcc actcttgagg cggcgggcca agccgcgccg cctgccatgc
     2701 atccgcgggg caatgtgctg cggctgtcgg tcgcgcaggc gctcgccggc gcgaactcga
     2761 cgctggtgta cgccgccgcc gcgatcgtcg gccacacgct ggcgcccagc aagcttctcg
     2821 cgacgctgcc gatctcggtt tacgtcgtgg gcatggcggt ctgcaccttg ccggccggcg
     2881 ccgtcgccag gcgctacggt cgccgcgccg cgttcctgac aggcacggga tgcggtgcga
     2941 tcgccggcct gctttccacc ctggcgatcc tgctgaatgc cttctggctg ttctgcatcg
     3001 gaacgtgctt cggcggtgcg tatggcgcgg tcgtgctgtc ctaccggttt gccgccgccg
     3061 attgcgtccc gccggaacgt cgcgcccgcg cgctggccgc ggtcatggcc ggcggcgtgt
     3121 tcgccggcgt gatcggcccg caactcgtca cctacacaat ggagctgtgg cagccctatc
     3181 tgttcgccgc caccttcctg gcacaggccg cggtggcggt gctgtccgcg ctgatcgtcg
     3241 ccggcgtgcg gctgccgaag ccgagcgcgg cggagatcgt cggcggcggg cgcccgctcg
     3301 gcgtcatcct gcgcgagccg cggttcatca tcgccatgat ctgcggcacc gtcgcctaca
     3361 tgctgatgaa cttcctgatg accaccgcgc cgctggccat gcgcctgtgc ggcatatcgc
     3421 agagcgcggc gaatctcggc ctgcaatggc acgtcatcgc catgtatgcg ccgagcttct
     3481 tcaccggacg gctgatcggc cggttcggcg cgccgcgcgt ggtggcggtg ggcctgacgc
     3541 tgttcgctgc cgccggcgtc atgggcctga ccggcaccga cgtgacgcat ttctggctga
     3601 gcctgatcct gctcggcctc ggctggaact tcagctggct gggcgcctcg gccctggtgc
     3661 tggaatgcca ccggccggag gaacggaccc gcgtgcagtc gctcaacgac ttcgtggtgt
     3721 tcggcatgat ggcgctgggg tcattctcgg cgggcgggct gctgctgagc ttcggctggg
     3781 acacggtgat ctgggtgtcg ttcctgccgc tggcggcggc ggttgccgca ctcgggtacc
     3841 gagccgcggc gcgttcccgt ctgttccccg ccgcgtgacg ctcccacggc gcgacggatc
     3901 actcaccgag aggaggctgc catgatcacc gagatcatcg tcttcgacct gcccgaggga
     3961 atgacccggg acgaggttgt ggccaattac cgccgcagcg cgccgggctg gcgcgccaac
     4021 cccgacctga tccgcaagaa ctacctgtac gacgccgagg gacgcagagc cggcggggtc
     4081 tatctctggc gcgacatgga cgcggcgcgg cgcgcacgcg atgcggcctg gctcgagcgg
     4141 gttcgccgca cctatggcag cgagccggtg gtgcagtatt tcgcgacgcc gctgctggtc
     4201 gacaacgcgc tggaccgcac catcgaggaa gccgacgcac gcgatcacgc cccggcctga
     4261 ttggcgcacc atccggcacg tctttggctg accgcagcct cagcccgggc ggacggacga
     4321 cgccgagcgt gagtcgccga tccgccgcgc cagctcggcg cggcaggcgc cggcatccgc
     4381 gacgcagccg ccgtccagcc accagc
//
LOCUS       DASSZH010000669         6853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_254884, whole genome shotgun sequence.
ACCESSION   DASSZH010000669 DASSZH010000000
VERSION     DASSZH010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6853
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254884"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1498)
                     /locus_tag="VFN42_14445"
     CDS             complement(<1..1498)
                     /locus_tag="VFN42_14445"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8997865.1"
                     /translation="MHDTVIRGGTILDGTGKARFTGDVAIDGDRIAQVGGKAGPGKRE
                     IDADGLLVTPGWVDAHTHYDGQATWDQELAPSSWHGVTTILFGNCGVGFAPVRREHHD
                     SLIGLMEAVEEIPGIALAEGLKWDWQSFPEYLDALERLPHAIDFGAQIPHHPLRVFVM
                     GDRAINREPATADDIAEMQRLTRQAMQAGAFGFTTSRTNSHKTLTGEMVPGRYSEEDE
                     LVGIGAALKGLGYGAFGVNSDFDDEASELAWMTRLSKDTGRPVWFLLTDRAKDPPRWR
                     RLLDGVHAARAQGAMVTAQVAGRPVGVLLGIDTALNPFSIRPSYGELLKMPADERLRR
                     LQDPAMRAQILSEAPSPELVNRLTQFRQQITTRWDRMFVMGDPPDYEPEESSSIASIA
                     RRGNHTPDEVAYDYLAEGLDRFLFFPIVGYTQDNHDNIRTMLTEPGTILGLSDGGAHC
                     SSITDASVPSWMLIHWARDRKRGPRLPLEMLVKRQTSETADFFGLTDRG"
     gene            1806..2621
                     /locus_tag="VFN42_14450"
     CDS             1806..2621
                     /locus_tag="VFN42_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997866.1"
                     /translation="MLRTIAGITRGDTRRRPVAPPSPSKPPTVRHPQAPAQPRPRAAR
                     RPRTASPAAPSQPSRSGWLARWFGRKRHRAAPPARHSRRSDDAPFTPETHPWLSPEAC
                     AFLNTPVEACDPALLRCILAAFALDLADNLPSDLGMDAKALFATLCRRLGALPDEANT
                     EATPAAPPGVAPAAAEHATQDADPAGLQQAPGMQTAPDDANAAAETASAPVGGTAPVF
                     QRPRLRSGAERQFGRRRPSPVRLAHQGCCHAAADAPRRLPPRRLYYAACAGPP"
     gene            complement(2797..4635)
                     /locus_tag="VFN42_14455"
     CDS             complement(2797..4635)
                     /locus_tag="VFN42_14455"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8997867.1"
                     /translation="MTRRAAKPLPWLAGLLAAYLIAPLIAGAQQLGLADWRSVHAGEL
                     LHACAVSIASASVSTLLIALGGIPLGYLLARSRGRAISSLGFLVQLPLALPPLTSGVL
                     LLFLIGYTTPLGQLAQGWLTDSFTGIVLAEVFVAAPFLIIAARSGFAAMDPVLEGVAA
                     TLGRKPLDVFLRVSLPIAWPAILSGLLLAWLRAFGEFGATVMVAYHPYSLPVYTYVAF
                     GSQGLPAMMPVLLPTLVVALVIMGLSGLAADKRPSGGERAIRGSVTTPLPRVALVPHP
                     APVRSADLRLQIEKRLGGFLLQVGWETRARRLAILGPSGSGKSLILKLLAGLDRCDRG
                     SILLEGRDVAPLDPAARHIAYVPQNYGLFPHLTVAEQLRFPAGADPALAARWIERLGL
                     RGLESRRPGELSLGQQQRVALARALTWPAQLLLLDEPFSALDAPLRARLRRELLALQQ
                     EFSATTILVTHDPLEAALLADEVLVLEQGRALQAGPTDRLFRRPANEQVARLLGADNV
                     AAGVVRDAQHIMVGDGVSLAVSGPPLTPGERVGWSFSPSRARISATGHYEGRVEAVAQ
                     MGVGRQITVRFGDARIRIFDGETDAPPDDPCRFEIDPHSVQVWPLH"
     gene            complement(4632..5483)
                     /locus_tag="VFN42_14460"
     CDS             complement(4632..5483)
                     /locus_tag="VFN42_14460"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8997868.1"
                     /translation="MAAGAGLLGQAAPASAAGTVKVLYAGSLVNLMEHGIGPAFHKAT
                     GDQFQGFAGGSDKEAHEIKARLRQGDVFISANPTVNRELTGAANGDWVRWYIAFAQSP
                     LVIGYNPSSRYAQAFKTMPWYKVLEQPGIRIGRTDPKLDPKGRLTVALLKRAASYYKL
                     PDLDRQVLGAAENPAQVFPEETLIGRLQSGQLDVGFFYSTETSEAKIPAIPLPPVIAP
                     KAVYTATILRGAPNPGGATRFVSFLLGAEGQRLMRGHGLTLRKPSVEGDAKAVPGPIQ
                     AMVADAK"
     gene            5813..6196
                     /locus_tag="VFN42_14465"
     CDS             5813..6196
                     /locus_tag="VFN42_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET8997869.1"
                     /translation="MGKRPPSQSNSAGKSDRLSIRLDLASGARIGPGKVAVLEEIARS
                     GSISAAGRALHMSYRRTWELVEDLNRSLGTPVVETAAGGSGGGGAVLTRAGKAVVTGY
                     RAIELDTALAARKHLVAISRACSEK"
     gene            6326..6742
                     /locus_tag="VFN42_14470"
     CDS             6326..6742
                     /locus_tag="VFN42_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HET8997870.1"
                     /translation="MTEHVRITAGPFSFLARFETEAAPKTCAMFRALLPYRQRIIHVR
                     WSGEACWIPLGEQAFGVGYENPTSYPAPGHILLHPGSVSETEILLGYGAVRFASKVGQ
                     LAGNHFLSIEQGLPDLPTLGKLVLWEGAQDIVFERG"
BASE COUNT         1133 a         2323 c         2303 g         1094 t
ORIGIN      
        1 gcccgcgatc ggttagacca aagaagtccg ccgtctcact ggtctgacgc ttcaccagca
       61 tttccagcgg aagccttgga ccacgcttcc ggtcccgcgc ccaatggatc agcatccagc
      121 tcggcacgct cgcatcggtg atcgacgagc aatgcgcgcc gccatccgac aatccgagga
      181 tcgttcccgg ctcggtcagc atggtgcgga tgttgtcgtg attgtcctgc gtgtagccga
      241 cgatcgggaa gaacaggaag cggtccaacc cttcggccag gtagtcgtag gccacctcgt
      301 ccggcgtgtg gttgccacgc cgggcgatcg aggcgatgga gctgctctcc tccggctcgt
      361 agtccggcgg atcgcccatg acgaacatgc ggtcccagcg cgtggtgatc tgctggcgga
      421 actgcgtcag ccggttcacc agttccggtg acggcgcctc gctgaggatc tgtgcccgca
      481 ttgctggatc ctgcagccgc cgcagccgct catccgccgg cattttcagc aattccccat
      541 agctcggccg gatggagaac ggattcagcg cggtgtcgat gcccagcaac acacccacgg
      601 gacggccggc gacctgcgcc gtcaccatcg cgccttgggc gcgggcggca tgcacgccgt
      661 ccagcaggcg ccgccaacgc ggcggatcct tggcgcggtc ggtcagcagg aaccacaccg
      721 gccggccggt gtccttcgac agccgcgtca tccaggccag ttcgctggct tcgtcatcga
      781 agtcgctgtt cacgccgaac gcgccgtagc ccagcccctt cagcgcggcg ccgatgccga
      841 ccaactcgtc ctcttcggaa tagcggccgg gcaccatctc gcccgtaagg gtcttgtgcg
      901 agttggtgcg cgacgtggtg aaaccgaacg caccggcctg catcgcctgc cgcgtcagcc
      961 gctgcatctc ggcgatgtcg tccgcggtgg cgggttcgcg gttgatcgcg cgatcgccca
     1021 tgacgaacac gcgcaacgga tggtgcggaa tctgcgcgcc gaaatcgatc gcgtgcggca
     1081 ggcgttccaa cgcatcgaga tattccggga aactctgcca atcccacttg agcccttcag
     1141 ccagcgcgat gccggggatt tcctccaccg cttccatcag cccgatcagc gagtcgtggt
     1201 gttcgcgccg caccggtgcg aagccgacgc cgcaattgcc gaacaggatg gtggtcacgc
     1261 cgtgccatga cgacggcgcc agttcctggt cccaggtggc ctggccgtcg taatgcgtgt
     1321 gcgcatcgac ccagccgggc gtgaccagca gaccgtcggc gtcgatctcc cgcttgccgg
     1381 gccccgcctt gccgccgacc tgggcgatac ggtcaccgtc gatcgccacg tcgccggtga
     1441 aacgggcctt gccggtgccg tccaggatgg ttccgccgcg aatgaccgtg tcgtgcattg
     1501 gcgctcgctc ccgttgcttc tgcttggttg ttgccgggat gggacgccgg aacggcggag
     1561 gggtcaagtt cgccgggcgg cgcgccagct tgttcgttcc tcacgccgcc gcggagaagc
     1621 cctggagcgc ggccgaagga ggcggaaaga gaaatatttc caggatatcc gtgacgcccc
     1681 cttgacaata ggggaaaaaa tgcatatagt gccgacccat gaacaaccca cccttccctg
     1741 ctggtctttc ttcccaaacc caccaagggg ttggtgcgcg ctttttccgc cgctgcggaa
     1801 aggcggtgct caggaccatt gccggcatca cccgcggcga cacccggcgg cgccccgtcg
     1861 cgccgccgtc cccctccaag ccgccaaccg tccgccaccc gcaagcgccc gcgcagccga
     1921 ggccgcgcgc cgcacggcgg ccgcgcacgg cgtcacccgc cgcgccgtcg caaccatccc
     1981 gctccggctg gctggcgcgc tggttcggcc gcaagcggca ccgcgccgcg ccgcctgccc
     2041 ggcattcccg gcgcagcgac gacgccccgt tcaccccgga aacccacccc tggctcagcc
     2101 cggaagcctg cgccttcctc aacacgcccg tcgaggcgtg tgatcccgct cttctccggt
     2161 gcatcctggc tgcgttcgcg ctggacctcg cggacaacct gccgtccgac ttggggatgg
     2221 atgccaaagc gctgtttgcc acgctgtgtc gccgtctggg cgccctgccc gatgaggcca
     2281 acaccgaggc gacgcctgcc gcgccgcccg gtgttgcgcc ggcggccgcg gagcacgcga
     2341 cccaggacgc agacccagcc ggtctgcagc aggcgccagg catgcagact gcgccggacg
     2401 acgccaacgc cgcggcggag accgcgtcag cgccggtcgg cgggaccgca cctgttttcc
     2461 agcgtccccg actccggtcc ggcgccgagc ggcagttcgg ccgtcgccgt ccgtcgcctg
     2521 tccgtctcgc tcaccagggc tgttgccatg ccgccgctga cgcgcctcgg cgcttgccgc
     2581 cgcggcgcct gtactacgcc gcttgcgccg gaccgccctg attcgtcgcg cgcccgttgc
     2641 ggcgcgggcc ggccgccgtg gcgtgccgca gcggctgcct cggctggtca aaatcagcct
     2701 ggtgctgacc tgggcgacac tgtccccggg catgttcgag cgcgctgact acggtagcgc
     2761 tcgacgctgt ggcacgcccc ccggcgtgcc gatccatcaa tgcagcggcc aaacctgaac
     2821 ggaatgcggg tcgatttcga accggcacgg atcatccgga ggcgcgtccg tctcgccatc
     2881 gaagatccgg attcgtgcgt cgccgaagcg caccgtgatc tggcggccga cacccatttg
     2941 cgcgacggct tcgacccgcc cctcataatg gcccgtggca ctgatgcggg cgcgcgaggg
     3001 ggagaagctc cagccgacgc gctcgcccgg cgtcaagggc ggccccgaca cggccagcga
     3061 aacgccatcc cccaccatga tgtgctgcgc atcgcgcacc acgcccgcgg cgacgttgtc
     3121 ggcgccgagc aggcgcgcca cctgctcgtt ggccgggcgg cgaaacagcc ggtcggtggg
     3181 accggcttgc agggcgcgcc cctgttccag gaccagaact tcgtcggcca gcaacgcggc
     3241 ttccagcgga tcgtgggtca ccaggatggt ggtggcgctg aattcctgct gcaacgccag
     3301 cagttcccgc cgcagccgag cgcgcaacgg tgcgtcaagc gcggaaaacg gctcatccag
     3361 cagcagcaat tgcgccggcc aggtcagggc gcgtgccagc gcgacgcgct gctgctgccc
     3421 gagcgacagt tcacccgggc ggcgggattc caggccgcgc agcccgagcc gctcgatcca
     3481 ccgtgcggcc agagcggggt ccgcgccggc cgggaagcga agctgctccg ccacggtcag
     3541 atgcgggaac aatccgtagt tctgcggcac gtaagcgatg tgacgcgcgg caggatcaag
     3601 cggcgcgaca tcacggcctt ccagcaggat gctgccgcgg tcgcagcggt ccaggccggc
     3661 cagcagtttc aggatcagcg atttgccgga tcccgaggga cccaggattg ccagccggcg
     3721 cgccctggtc tcccagccga cctgcagaag aaacccgccg agccgctttt cgatctgcag
     3781 ccgcagatcg gccgaccgga ccggtgcggg gtgcggcacc agagccaccc gtggcagcgg
     3841 cgtggtcacg gacccgcgga tggcgcgctc tccgcctgag ggacgcttat ccgccgcaag
     3901 cccgctcagc cccatgatga ccagcgcgac caccaaggtg ggcagcagta ccggcatcat
     3961 cgccggcaag ccctgcgacc cgaaggcaac gtaggtgtag accggcaagg aatacggatg
     4021 gtacgccacc atgaccgttg cgccgaactc gccgaatgcg cgcagccagg cgagcagcag
     4081 tccggacagg atggcgggcc aggcaatcgg caggctgacg cgcaggaaga catccagcgg
     4141 cttgcggccc agcgtggcgg cgacgccttc cagcacggga tccatcgcag caaagccgga
     4201 ccgcgccgcg atgatcagga acggcgcggc gacgaacacc tcggcgagca cgatgccggt
     4261 gaaggaatcc gtcagccagc cttgggcaag ctggccgagc ggcgtggtgt agccgatcag
     4321 gaacagcaac agcacgccgc tggtcagcgg cggcaaggcc agcggcagct gcacgagaaa
     4381 gcccaggctg gatattgccc tgccgcgcga gcgcgccagc agatagccga gcggaatgcc
     4441 gccgagcgcg atcagcaagg tggagacact ggcgctcgcg atcgacacgg cgcaggcatg
     4501 cagcagttcg ccggcgtgca ccgaacgcca atcggccaac cccaattgct gcgcaccggc
     4561 aatcagcggt gcgatgagat acgccgcgag caggccggcc agccacggca gcggcttggc
     4621 agcgcgccgt gtcacttggc gtcagcgacc attgcctgga ttggccccgg cacagccttg
     4681 gcatcccctt cgacgctggg tttgcgcagg gtcaggccgt gtccgcgcat cagccgttgt
     4741 ccctccgcac ccagcaggaa gctcacgaag cgggttgctc cgccaggatt gggcgcgccg
     4801 cgcaggatgg tcgcggtata gacggctttc ggggcaatga ccgggggaag ggggatggca
     4861 ggaattttcg cctcggatgt ttcggtcgaa tagaagaagc cgacgtcaag ctgccccgat
     4921 tgcaggcggc cgatcaaggt ctcctccggg aagacctgtg ctgggttttc cgccgcgccc
     4981 agcacctgcc ggtcgagatc cggcagcttg taataggatg ccgcgcgctt cagcaatgcc
     5041 acggtcaacc ggcctttcgg atcgagcttt ggatcggttc gcccgatgcg gatgccaggt
     5101 tgctccagca ccttgtacca gggcatggtc ttgaaggctt gggcatagcg gcttgaggga
     5161 ttgtagccga tcaccagcgg ggactgggca aaggcgatgt accatcgcac ccagtcgcca
     5221 ttcgccgccc cggtcaattc gcggttcacc gtagggttgg cgctgataaa cacgtcgccc
     5281 tggcgcagcc tggccttgat ttcgtgcgct tccttgtcgg aaccgccggc aaacccctgg
     5341 aactggtcgc ccgtggcctt gtggaaggcg ggaccgatgc catgctccat caggttcacc
     5401 agcgacccgg cgtacagcac cttcaccgtg ccggcggcgg aggcgggtgc ggcctggccg
     5461 agcaagccgg cgcccgcagc cattgcgaag gttgcgcgaa gcagcgaacg acgggtgggt
     5521 atatggcgct ggcagaccag cgtggggtgg gcagtcatga tcggcacttc cggttccgtg
     5581 ggcttcaggc gtatcgtttc ggagcgcgcc attggcgcca tgcctccgct tcgctggcgt
     5641 ggagtggcta tagcgtgagg cggctcagtg cggtgaaatt tgcggtatag gggcgaatcc
     5701 atgcccctcg ttggcgacga tatatccagt tggatatatc gccaagaggt atgggatgcc
     5761 cggaccgctg cggttccggt acgtgggaac gggtgcagcc gggatagcag ccatgggaaa
     5821 gcgaccgcca agccaatcca actcagccgg caagagcgac cggctgagca ttcgtctcga
     5881 ccttgcctcg ggtgcccgca ttggcccggg caaggtggcg gtactggagg aaattgcccg
     5941 ctccggttcg atttccgctg ccgggcgtgc cctgcacatg tcgtaccgac gcacctggga
     6001 gctggtcgag gacttgaacc gttcgctcgg cacccccgtg gtggaaaccg cggcgggcgg
     6061 cagcggcggt ggcggtgcgg tgctgacccg cgccgggaag gccgtggtga ccgggtatcg
     6121 tgcgatcgaa ctcgacactg cgctggcggc ccgcaagcac ctggtggcaa tcagccgagc
     6181 ctgttccgag aaatgacccg aaggcgggtg ggtgcgcgcc gcgcgatctg ctacgccagt
     6241 agcgcggctc tgtccgccgc aggcgatggg gagtgcaggc gatgaagctt ggcaaggtgg
     6301 cgcgcaaccc gggagttggt ccgcaatgac ggagcatgta cgcatcacgg cgggaccgtt
     6361 cagtttcctg gcgcgcttcg aaaccgaggc ggcgccgaag acctgcgcga tgttccgcgc
     6421 gctgctgccc taccggcaac gcatcatcca tgtgcgctgg agcggtgagg catgctggat
     6481 tccgttgggc gaacaggcct tcggtgtcgg ctacgagaat cccaccagct atccggctcc
     6541 gggacacatc ctgctgcacc cgggcagcgt cagcgagacg gagattctgc tgggttacgg
     6601 tgcggtgcgc ttcgccagca aagtggggca acttgccggc aaccacttcc tcagcatcga
     6661 gcaggggctt ccggacctgc caacgctggg caagctggtg ctgtgggaag gcgcgcagga
     6721 tatcgtcttc gagaggggct agatgttgga gattggagat tggagattag ggactggagt
     6781 tcagcaggaa ttgagaatgc gccatctctc gtctctaatc tctagtccct aatcgctagt
     6841 ctctcgtttc cag
//
LOCUS       DASSZH010000670         5182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1089923, whole genome shotgun sequence.
ACCESSION   DASSZH010000670 DASSZH010000000
VERSION     DASSZH010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089923"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="VFN42_14475"
     CDS             complement(<1..902)
                     /locus_tag="VFN42_14475"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF016120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HET8997871.1"
                     /translation="MPMFARGAGVAPPAELVVGLVNNMAPATRRTTEQQFAGILAAAS
                     KTLDMRVRLLAVENLSDGTADALRVLQQARPDALIVTGAEPQCGSLAEEPLWPALARL
                     ADWAAEHTTSAIWACMAAHMVVYRLDRISRRRLPQKLSGVFACRRMTNHGLLANAPAA
                     WTVPHSRHNGLDEMELRGNGYTILSHGGPRVGPDSFVRQAGGSLFLMLQGHPEYGRDS
                     LRGEYRRDVRRFLAGRRDSYPDVPHGYFDEETTAALAELRGQAGRIPDAALLAALDAA
                     VAVAPDHAWHGPAVRLFANWLSYLA"
     gene            complement(908..2239)
                     /locus_tag="VFN42_14480"
     CDS             complement(908..2239)
                     /locus_tag="VFN42_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HET8997872.1"
                     /translation="MRPETIALHAGYQADMSVRSVAVPIYQTVAYEFDSAAHAAALFN
                     LEAEGFRYSRISNPTTSVLEQRIAALEGGVGALCLASGQAALHAAILGIAEQGRNIVS
                     VPQLYGTTHTLFAHVLPRMGVGVRFAASDEPDDLEQLIDDDTIAVYCESVGNPAGNVC
                     DIEALATMAHARGLPLLVDNTVASPMLLRPIEFGADIVIHSLTKFLGGHGTTLGGAIV
                     DGGRFPWHRHAGRFPALNEPDPSYHGLVYAEHFGPTAYIERCRNFALRTTGAALAPMS
                     AFLLLQGVETAALRVERHVQNAQAVARALQKDPRVAWVNYAGFPESPYYPLVRKYLGG
                     RACSLLTFGVNGGMPAGMRFYDALRLIKRLVNLGDAKSLVCHPASTTHRQMTAQEQAK
                     AGVTPELIRLSVGIEHVDDILADIDQALAAAQSDRFAPLPSDDRLCEEKTL"
     gene            2497..3210
                     /locus_tag="VFN42_14485"
     CDS             2497..3210
                     /locus_tag="VFN42_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997873.1"
                     /translation="MSAMHIKTELAYERVIAEICGFNWINLAEEDMVHVAWAYYFFSV
                     QFRKNLQVARILYPEDGKLQELEREECDTDNLSPWPGVARVGEKMDHDEFMRRVLLLS
                     PVPEAQRRRFEASGHRYLDAVRRTDATTRALSITSYEDGGLERVFTAMLTSPESHDPL
                     LRGFRHFLAEHIRFDSDPDGGHGAMIRHLRPDDHILPLWIGFRDILLEFAPGLANDHD
                     GSGMFRHDRDALMQQVQPA"
     gene            3310..3555
                     /locus_tag="VFN42_14490"
     CDS             3310..3555
                     /locus_tag="VFN42_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997874.1"
                     /translation="MSIRSTILSQIAEIAEQQKKRLAPLTDGLPLLESGLDSLCIAIL
                     VASLDDRLDMDPFSREDIPKLPVTLGDFIALYEHAAA"
     gene            3542..4957
                     /locus_tag="VFN42_14495"
     CDS             3542..4957
                     /locus_tag="VFN42_14495"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HET8997875.1"
                     /translation="MRRPEHRSLWDLTAAAGLTRGRVLADADGCVRLADLASAGSLDV
                     PVEKLRGQSVLISCARQLPFALALLQLDGVAGRIVLCPPDLATEQLGAVMADAEVTAI
                     VSDGTGPAAHRSAAAAGATPVFSCDADPRPGRAIPAAGGDDRHVETEWLLLTSGTSGR
                     PKLVVHTLASLVGPLPADAGGAKDAVWSTFYDVRRYGGLQILLRALLGGGSMVLSQAG
                     EGMAAFLARTGAGGVTHISGTPSHWRLALMSSAADRIAPRYIRLSGEIVDQAILSNLA
                     AAFPASSIAHAFASTEAGVAFEVRDGLAGFPAALIGEQRGGVELRVENGSLRIRSARI
                     ARRYLGDCRLAVDADGFLDTGDMLELRDGRCHFVGRREGVINAGGKKVHPEEVEAVIN
                     RQRGVQMARVRGRPSPITGALVVADIVVRPSAGDSVETIKADILAACRAALPPHKVPA
                     MLQAVASLDVAGSGKLARQHA"
     gene            4950..>5182
                     /locus_tag="VFN42_14500"
     CDS             4950..>5182
                     /locus_tag="VFN42_14500"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8997876.1"
                     /translation="MRNVLVTGGSRGLGLGITRALAFAGFQVVAIARQEGDQLPALRA
                     LAEREACGTVHFRPFDLADIGGIPGLVKSLSREL"
BASE COUNT          828 a         1704 c         1768 g          882 t
ORIGIN      
        1 gccagatagg acagccagtt tgcgaacagc cgcacagccg gcccgtgcca ggcatggtcc
       61 ggcgcgaccg cgacggcggc gtccagggcg gcaagcaggg ccgcgtccgg aatccgcccg
      121 gcctgcccgc gcagctccgc cagcgccgcc gtggtttctt cgtcgaaata gccatgcgga
      181 acgtccgggt agctgtcgcg ccttccggca agaaagcgcc tgacgtcgcg gcgatattcg
      241 ccgcgcaggc tgtcgcggcc gtattccggg tggccctgca gcatcaggaa caggcttccg
      301 cccgcctgcc tgacgaaact gtcggggccg acgcgcgggc cgccatgcga caggatcgta
      361 tagccgttgc cgcgcaattc catttcatcg aggccgttgt gccgcgaatg cggcaccgtc
      421 caggccgccg gcgcgtttgc cagcagcccg tgattcgtca tcctcctgca cgcgaacacg
      481 cccgacagtt tctgcggcag tcgcctgcgg ctgatccggt ccagccggta caccaccata
      541 tgcgccgcca tgcaggccca gatcgcggac gtggtgtgtt cggccgccca gtccgccagc
      601 cgcgccagtg ccggccagag cggttcctcc gccaggctgc cgcattgcgg ctcggcgccg
      661 gtgacgatca gcgcgtcggg ccgggcctgc tgcaacacgc ggagagcatc ggcggtgccg
      721 tccgacagat tttcgaccgc gagcagcctg acccgcatgt ccagcgtctt tgacgccgcc
      781 gccaggatgc cggcgaattg ctgctccgtc gtgcgtcggg tcgccggcgc catgttgttg
      841 acgagaccga cgaccagttc cgccggcggc gcaacgcccg ctccgcgcgc gaacatcggc
      901 atgccgttca aagggtcttt tcctcacaga gccggtcgtc ggagggcagc ggcgcgaacc
      961 gatcggactg cgccgcggcg agcgcctgat cgatatcggc aaggatgtcg tccacgtgtt
     1021 cgatcccgac gctgagccgg atcagctcgg gcgtgacccc cgccttcgcc tgctcctgcg
     1081 ccgtcatctg ccgatgggtg gtcgatgccg ggtggcagac gagggatttc gcatccccga
     1141 gattcacgag ccgcttgatc aggcgcaagg catcgtagaa tcgcatcccc gccggcatgc
     1201 cgccgttcac gccgaatgtc agcagcgagc atgcgcgacc gccgaggtat ttccgcacca
     1261 gcggatagta cgggctctcg gggaagcccg cgtagttgac ccaggcaacg cgaggatcct
     1321 tctgcaacgc cctggcgacg gcctgcgcat tctgcacgtg ccgctcgacg cgcaaggcgg
     1381 cggtctccac gccctgcagc agaagaaagg cgctcatcgg ggccagggcg gcgcccgtcg
     1441 tgcgcagggc gaagttgcgg catcgctcga tataggccgt gggcccgaaa tgctccgcgt
     1501 acaccagccc gtgataggac ggatccggtt cgttcagggc cgggaaccgt ccggcatggc
     1561 ggtgccaggg gaagcgcccg ccatcgacga tggcgccgcc gagcgtggtg ccgtggccgc
     1621 cgagaaactt ggtcagcgag tggatgacga tatccgcgcc gaactcgatc ggccgcagca
     1681 gcatcggtga cgccaccgtg ttgtccacca gcagcggcag ccccctggca tgcgccatcg
     1741 tggccagcgc ctcgatatcg cagacatttc cggcgggatt gccgacgctc tcgcaataga
     1801 cggcgatggt gtcatcgtcg atcagttgct caaggtcatc cggctcatcg gacgcggcga
     1861 agcgcacgcc gacgcccatg cgcggcagca catgcgcgaa cagcgtgtgg gtggtgccgt
     1921 agagctgggg gacggagacg atgttgcgtc cctgctccgc gatgccgaga atggcggcgt
     1981 gcaacgcagc ctgtcccgac gccaggcaca acgcacccac gccgccttcg agcgcggcga
     2041 tgcgctgctc aagcacggat gtggtcggat tgctgatgcg gctgtagcgg aaaccctcgg
     2101 cctcaagatt gaacagggcg gcagcgtgtg ccgcgctgtc gaattcatat gcgacggtct
     2161 ggtagatcgg caccgccacg gacctgaccg acatgtcagc ctggtacccg gcatgcaggg
     2221 caatcgtctc gggccgcatt gcaaactcct cgcgaaaggg ctgtgccgcc cgcggccggc
     2281 gggcgcgtca acgcatgcgg cagttagcag aggaccaaac cgttctgata tacaaccagt
     2341 tacgatctcg cctgtttttg atgccgtgtc ctcgaccggc ggcgtcgcgc gctgacgatt
     2401 agtttaacgg tacgcaacgg attagcgttg caatgacaaa gctatctcga caacgatact
     2461 tcagtccgga aaccaggaac ggtcgtgagg tgggtcatgt ctgcgatgca tatcaagacg
     2521 gaactggcct atgaaagggt cattgccgaa atatgcggct tcaattggat caatcttgcc
     2581 gaagaagaca tggtccacgt cgcgtgggca tattattttt tttcggtgca gttccgcaaa
     2641 aacctgcagg tggcgcggat cctttatccg gaagacggga aattgcagga gcttgagcgg
     2701 gaggaatgcg acaccgacaa cctctcgccg tggcccggtg tcgcccgcgt gggcgagaag
     2761 atggaccatg acgagttcat gcggcgtgtc ctgctgctct cgccggttcc ggaagcgcag
     2821 cgccgccggt tcgaggccag cggacatcgc tacctggatg cggtgcgcag gacggacgcg
     2881 acaacgcggg cgctgagcat cacaagctat gaggacggcg ggcttgagcg ggtgtttacc
     2941 gccatgctga cgtcgcctga atcgcacgat ccgctgttgc gcggattcag gcatttcctc
     3001 gccgagcaca tcaggttcga cagcgacccg gatggaggcc acggcgcgat gatccgccac
     3061 ctgcggcctg atgatcacat ccttccgctg tggatcggct tccgggacat tctgctggag
     3121 ttcgcgccgg gattagcaaa cgaccatgat ggttccggca tgttccgcca cgatcgggac
     3181 gcgctgatgc agcaggtcca acccgcctag caggaagccg acgcgcgcgc ggaacgcacc
     3241 atacccgcgt cagcgcgggt ttccgcgccg gaagtccggt cattcgcagc gaatccgtac
     3301 gaggtgtcca tgtcgatccg ctccaccatc ctctcccaga tcgccgagat cgccgaacag
     3361 cagaagaagc gtctggcgcc gctgaccgat ggcctgccgc tgctcgaatc cgggctcgac
     3421 tcgctctgca tcgcgatcct ggtcgcttcg ctggacgatc ggcttgatat ggatccgttc
     3481 tcccgcgagg acataccgaa actgcctgtc actctcggcg atttcatcgc gctgtatgaa
     3541 catgcggcgg cctgagcacc ggtcgctgtg ggacctgacg gcggccgccg gactgacgcg
     3601 cggacgcgtc ctggccgacg cggatggctg tgtccgcctg gccgatctgg cgtccgccgg
     3661 cagtctggac gtcccggtgg agaagctgcg tggccagtcc gtgctcatct cctgcgcccg
     3721 gcagctgccc tttgcgctgg cgctgctgca actcgacggc gtggcggggc gcatcgtgct
     3781 gtgcccgccg gacctggcca cggaacaact gggcgcggtc atggccgacg cggaggtgac
     3841 tgcgatcgtc agcgacggca ccggcccggc ggcgcaccga tccgccgccg ctgcgggcgc
     3901 gacgccagtg ttttcctgcg acgccgatcc tcgtccgggc cgggcaattc cggcggcggg
     3961 tggcgatgat cgccacgtgg agaccgaatg gctgttgctg acctcgggca caagcggccg
     4021 gccgaagctt gtggtgcata ccttggccag tctcgtcggg ccgcttcccg ccgacgcggg
     4081 tggtgcgaaa gacgcagtct ggagcacgtt ctacgacgtc cgccgctatg gcgggctgca
     4141 gatcctgctg cgtgccctgc tgggcggcgg ctcgatggtg ctgtcgcagg ccggggaggg
     4201 aatggcggcg ttcctggccc gcaccggagc ggggggcgtc acccatatat cgggaacgcc
     4261 gtcgcactgg cgtctggcct tgatgagttc cgcggcggac cgcatcgcgc cgcgctacat
     4321 ccgtctttcc ggcgagatcg tggaccaggc gatcctgagc aatcttgccg cggcgtttcc
     4381 cgcctcctcc atcgcgcacg cctttgcctc cacggaggcg ggcgttgcgt tcgaggttcg
     4441 cgacgggctg gcgggatttc ccgccgcgct gatcggcgaa cagcgcggcg gggtggaatt
     4501 gcgggtggag aatggctcgc tgcgcatccg ctcggcccgc atagcgcggc gctatctcgg
     4561 ggactgcagg ctcgccgtgg atgcggacgg cttcctcgac accggcgaca tgctggagtt
     4621 gcgcgatggg cgctgccatt tcgtcggccg gcgcgaaggg gtcatcaatg ccggcggcaa
     4681 gaaggtgcat ccggaagagg tcgaggcggt gatcaaccgg caacggggcg tgcagatggc
     4741 gcgcgtacgc ggccgcccca gcccgatcac cggggcgctg gtggtcgccg atatcgtcgt
     4801 gcggccgtcc gccggggact cggtcgagac gatcaaggcc gacatcctgg cggcctgccg
     4861 ggcggccctg ccgccgcaca aggtgccggc catgctgcag gcggtcgcct cgctcgatgt
     4921 tgccggttcc ggcaagctgg ctcgccagca tgcgtaacgt gctggtgacc ggcggcagtc
     4981 gcgggctggg ccttggcatc acccgcgcgc tcgcgttcgc cgggttccag gtggtcgcga
     5041 tcgcccggca ggaaggcgat cagttgccgg cgcttcgtgc gctggcggaa cgggaggcgt
     5101 gcggcacggt gcatttccgc cccttcgacc ttgccgacat cggcggcatt cccggcctgg
     5161 tgaaatcgct gagccgcgag ct
//
LOCUS       DASSZH010000671         3915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_664320, whole genome shotgun sequence.
ACCESSION   DASSZH010000671 DASSZH010000000
VERSION     DASSZH010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664320"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1421
                     /gene="priA"
                     /locus_tag="VFN42_14505"
     CDS             <1..1421
                     /gene="priA"
                     /locus_tag="VFN42_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014457072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET8997877.1"
                     /translation="SSQWLERFEGRFGVAPAVWHSDLSSRVRRITRRAVADGAAPVVV
                     GARSALFLPFPDLGLIVIDEEHETAFKQEEGVVYHARDMAVVRARFCHAPAVLVSATP
                     SLETVANVEAGRYAKLHLPNRHGGATLPEVAAVDLRETPPERGHFLAPPLIEAVRQTL
                     GRGEQAMLFLNRRGYAPLTLCRHCGHRMQCPNCTAWLVEHRVRRELQCHHCGHAVPIP
                     PECPACGAPDTLTPVGPGVERITEEAAALFPDARRLVMASDTLPGPDAAGVAARAIAD
                     RQVDLIIGTQIVAKGWHFPHLTLVGVVDADLGLAGGDLRAAERTVQLLHQVAGRAGRA
                     EAPGRVLLQTFSPEHPVMQALVRGDLAAFMASEAAQRRPGHWPPYGRLAALIVSADAG
                     PVADALARDLSRAAPHAPGVEVLGPAPAPLAILRGRHRRRLLLKTRRDIAVQPILREW
                     LAKVPVPRGARVDVDVDPVSFL"
     gene            complement(1637..2590)
                     /locus_tag="VFN42_14510"
     CDS             complement(1637..2590)
                     /locus_tag="VFN42_14510"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HET8997878.1"
                     /translation="MFHTAICDLLGIRYPILQGAMQGGGGVELVAAVSEAGGLGVLPT
                     FGGTDAKLRDDIVAVRTRTAKPFGVNIMPMGQGIAERCAAACIELGVPIVTTGRADPG
                     EHLVRQLKAAGIKVVSVIPTVEHARRMEAEGVDAVVASGCEAGGHVGSVATMPLVPQV
                     VDAVTIPVLAAGGIGDARGFLAAFALGAVGIQMGTRFMATTESDLNAWGRSRLLEMRE
                     TDTIVTRAMTGATVRCIRTPEIVAYEEALTHHADESALKQLMRQVRGSRHSDNKGERR
                     QSAAGQIAGLILDIVPVGVLIETMLAEAVQLANRMPQIARR"
     gene            complement(2611..3630)
                     /locus_tag="VFN42_14515"
     CDS             complement(2611..3630)
                     /locus_tag="VFN42_14515"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET8997879.1"
                     /translation="MTDPHPAAEPPMTTVRLQNIAQSYGASAALMAAVEIGLFTAVSH
                     GAGTYQEVAAALGIHPTNAERLMVMLCATGLLKKTDDRHVNAADVERFLVQGKPGYMG
                     PWITFTKPQWNEWGRLSDHLRATNLRVMGSIGSFTEEDARRYHNATYSIGLGAGRRFV
                     RQVDLAHRRRIMDIGGGSGAYCIAAANAHSALTAVVLDLPPVCAIAREFIAENGLSDR
                     LTAQPCDFTRDPFPSDCDVAIMASNLPMYSREMVAMVVRKAHDALLPGGEMHLIGETI
                     SDDRTGPWGPAYWGLGQAVNDSLGLAHSEADVRGYFRAAGFEAVTVHEFIPGSLSRIA
                     GRKSS"
BASE COUNT          628 a         1346 c         1279 g          662 t
ORIGIN      
        1 tgtcgtcgca gtggctggag cgtttcgaag gccgcttcgg cgtggccccc gcggtgtggc
       61 actccgacct cagcagccgg gtgcgccgga tcacccgccg cgccgtcgcc gatggcgccg
      121 cgccggtggt ggtcggcgcc cgctcggcgc tgttcctgcc gtttcccgac ctcggcctga
      181 tcgtgatcga cgaggagcac gagaccgcgt tcaagcagga ggaaggtgtc gtctatcacg
      241 cgcgcgacat ggcggtggtg cgcgcccgat tctgccacgc gccggcggtg ctggtctccg
      301 ccacgcccag cctggagacg gtggcgaatg tggaggcggg gcgctacgcc aagctgcacc
      361 tgcccaaccg ccacggcggc gccacgctgc cggaggtggc ggcggtcgat ttgcgggaga
      421 cgccgccgga gcgcggccac ttcctcgcgc cgccgctgat cgaagcggtg cggcagacgc
      481 tggggcgcgg cgagcaggcg atgctgttcc tcaaccgccg cggctacgcg ccgctgacac
      541 tgtgccggca ttgcggccac cgcatgcaat gcccgaactg caccgcctgg ctggtggagc
      601 accgggtgcg gcgcgaactg caatgccacc attgcggcca cgcggtgccg atcccgccgg
      661 aatgccccgc ttgcggcgcg cccgacacgc tgactccggt cggccccggc gtggagcgca
      721 tcaccgagga agccgccgcg ctgttccccg acgctcggcg gctggtgatg gcgagcgaca
      781 ccctgcccgg gccggacgcc gccggggtgg cggcgcgggc catcgccgac cggcaggtgg
      841 acctgatcat cggcacgcag atcgtcgcca agggctggca ctttccgcat ctgacgctgg
      901 tgggcgtggt ggacgccgat ctgggcctgg caggcggtga cctgcgggcg gcggaacgca
      961 cggtgcagct gctgcaccag gtggcgggcc gcgccggccg cgcggaggcg ccgggccgcg
     1021 tgctgctgca gaccttctcg ccggagcacc cggtgatgca ggcgctggtg cgcggcgacc
     1081 tggcggcgtt catggccagc gaggcggcgc agcgccgccc cggccactgg ccgccgtacg
     1141 gccgtctggc cgcgctgatc gtcagcgccg atgccgggcc ggtggccgac gcgctggcgc
     1201 gcgacctgag ccgagcagcg ccgcatgcgc cgggcgtgga ggtgctcggc cccgcccctg
     1261 ccccgctggc gatattgcgc gggaggcacc ggcggcgtct gctgctgaag acgcggcgcg
     1321 acatcgcggt gcagccgatc ctgcgggaat ggctggcaaa ggtgccggtg ccacgcggcg
     1381 cacgggtgga tgtggacgtg gacccggtgt cgttcctgta gcgggggcct gtttgggcgc
     1441 ggccgttatg acacggtgag ctgttcgggg tcattctgtc ggtagaggcg ggcgctgcgt
     1501 tcgcgccacg ccacctcggc gtatggcatt cgcccggctc catgggtcag agccaggatc
     1561 tggtgcaatg tctgcgtcag gcttcgaatg ccggacagcc atgcctcaaa gcacgtccga
     1621 tcccccgcca aacggttcac cttcgagcaa tctgcggcat acggttcgcg agttgcaccg
     1681 cctcggccag catggtctcg atcaacacac caaccggtac gatatcaagg atcaacccgg
     1741 caatctgtcc cgctgccgac tgtctgcgct ctcccttatt gtcgctatgg cgcgaaccgc
     1801 gcacctgccg catcagctgc tttagcgccg actcatcggc gtgatgcgtc aatgcctctt
     1861 catacgcgac gatttccggc gtgcggatgc atcgcaccgt tgctccggtc atggcccgtg
     1921 tcacgattgt atccgtctct cgcatctcca gcaggcgcga gcggccccat gcattcaggt
     1981 cggattccgt cgtcgccatg aatcgggttc ccatctggat gccgactgcc ccaagagcga
     2041 atgcagccag aaacccgcgg gcgtcgccga tgccgccggc ggcgaggacc ggaatggtca
     2101 cggcatcgac cacctgcggc accagaggca tcgtcgccac cgacccgaca tgcccgccgg
     2161 cctcgcaccc cgacgcgacg accgcgtcaa cgccttccgc ctccatccgt ctggcatgtt
     2221 cgaccgtcgg gataaccgag acgaccttga tgcctgctgc cttgagctga cgcaccaggt
     2281 gttcgcctgg gtcggcacga ccggtggtga caatcggcac gccgagttca atgcacgccg
     2341 cggcgcatcg ttcggcgata ccctgtccca tcggcatgat gttgaccccg aacggcttcg
     2401 ccgtgcgtgt gcgaacggcc actatgtcgt cgcgcagttt cgcgtcggtg ccgccaaatg
     2461 tcggcaatac gccaaggcca ccggcttcgc tgacggcggc caccagttca acgccaccgc
     2521 cgccctgcat cgccccctga aggatcggat agcgaatgcc gagcaagtca caaatcgccg
     2581 tatgaaacat cacaggcccc tcgtgttggt tcagctggac ttgcggccag cgatacggct
     2641 caaggaacca gggatgaact catgcaccgt caccgcctcg aagccggccg cgcggaaata
     2701 gcccctcaca tcagcttccg aatgtgcgag tcccaaggaa tcgttcactg cctggccaag
     2761 cccccaatag gccggtcccc acggcccggt tcggtcgtcg ctgattgtct ccccgatcag
     2821 gtgcatctca ccgccaggca gcagcgcgtc gtgcgctttc ctgaccacca tcgcgaccat
     2881 ctcgcggctg tacatcggca ggttcgacgc catgatggcg acgtcgcaat cggacgggaa
     2941 cggatcacgc gtgaaatcgc aaggctgcgc cgttagccgg tccgacaagc cgttttccgc
     3001 gatgaattcg cgcgcgatcg cgcatactgg cggcagatcc agcacgacag cagtcaacgc
     3061 ggaatgtgcg ttcgcggctg cgatgcagta tgcccccgaa ccgcctccga tatccatgat
     3121 ccggcgtcgg tgtgccagat cgacctgccg aacaaagcga cggccggcac caagcccgat
     3181 cgaataggtc gcgttgtggt agcgtcgggc gtcctcttcc gtgaagctcc cgatgctgcc
     3241 catgactcgc aggttcgttg cccgcaagtg atcgctcaag cggccccact cattccactg
     3301 cggcttggtg aaggtgatcc agggacccat gtagcccggc ttgccctgca ccagaaaccg
     3361 ttcgacatcc gctgcattga catgacggtc atcggtcttc ttcagcagcc cggtcgcgca
     3421 tagcatcacc atcagacgtt cggcgttggt gggatggatg cctagcgccg ccgcgacctc
     3481 ctggtaagtg ccagcgccgt ggctgaccgc ggtgaagagg ccgatctcca ccgccgccat
     3541 gagtgcggca gacgcgccat aggactgagc gatgttttgc agtcgaacgg tggtcatcgg
     3601 aggctccgcc gcgggatgcg gatcggtcat ggtcgtctcc cttgcttttc tgcacgtgaa
     3661 gcacagcatg cgtgcggcca aagggaaacc tgatgaggca cctttctcgg ctgggcgtga
     3721 ccgtcgccgc tcattgccgc cttccgacct gcggtaatgc cgctcatgcg gcaaacgatt
     3781 cgacaccggt cagcggtgca gaaatcaaga aatccgcaac cttcctacgg ccttgaaaag
     3841 aaggcgaaaa cttgccgcga aattctggtg cataactgcc caatggtttc cttctccccg
     3901 ccggcgaagc ccacg
//
LOCUS       DASSZH010000672         2912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_766176, whole genome shotgun sequence.
ACCESSION   DASSZH010000672 DASSZH010000000
VERSION     DASSZH010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766176"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1269
                     /locus_tag="VFN42_14520"
     CDS             <1..1269
                     /locus_tag="VFN42_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HET8997880.1"
                     /translation="QLFKDFMNRQHPGQGGAAPQPEQHLQSLGSGFIIDPAGLVVTNN
                     HVIEGADQITVTLQDGTTLKASVVGRDDVGDIALLRVKPTKPLPSVSFGDSAKERVGD
                     WVLAIGNPFGLGGTVTAGIISARGRNIHQGPYDDFLQTDAPINQGNSGGPLFNMDGQV
                     IGMNTAIYSRSGGSVGIGFSIPSNMVKDVVEQLHQYGHPRRGWLGVRIQQVTPDIAES
                     LGLKQPRGALVAGVNDGGPADKAKLHNGDVILKFNNEDVKDMHALPRIVAEASVGKTV
                     PVVVWRDGKDVTLQATLAEKPDDIKTATAPAPKPALDQPTAIAGLGMKLAPLSPALRQ
                     KYQLGADQKGVVVTDVEPKGPAAERGLKPGDVIVEVQQGEVGTPAEVQQRIEGVRKEN
                     RKSVLMLIQRQDGLQWVPLPLSGGPEKQPG"
     gene            1342..2463
                     /locus_tag="VFN42_14525"
     CDS             1342..2463
                     /locus_tag="VFN42_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997881.1"
                     /translation="MLDDDDYDRLVAQIYAAAADASLLPALLEDLRAGFGGSAAALIA
                     FSEAPGQSRVVATRQEAMAEYRRHWDARNPLHPLRRTGGQLAPGTIATDAMLMPRDAL
                     IRTDYFNEFLLAYELPNLLAVKLAAGVGQDVTLNLFRGTRQGGFDPVHLKVARRLGPH
                     LHGATRIASRLAWAGVKAGGSDAALDRLVSGTVLLDRSGRVVHVNAAAERMLAEQDGL
                     QLANRRLTAKSPGEHAALARLIGRAAGEGDACGCGGITVSRPSGRRAWALIAAPLRVE
                     VAWLAPQRPAVVVSITDPEVTPTPAADRLVQLYGLTAREADVALGIARGAELREVADG
                     LGLTQLSARQYLSRALRKTGAHRQHDLTRLLVSLGAAAG"
     gene            2591..>2912
                     /locus_tag="VFN42_14530"
     CDS             2591..>2912
                     /locus_tag="VFN42_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997882.1"
                     /translation="MRVFRLIGLLGIAASLSGPAWATTYDAVTDFNATSNAGGNIWTY
                     RSGTTGSGTLLTGTSTTSPGSLPQWDGPGYPYISANNSATLYQGGTVLYEPNALHLDG
                     GGVGA"
BASE COUNT          482 a          934 c         1032 g          464 t
ORIGIN      
        1 cagctcttca aggacttcat gaaccgccag catcccggcc agggcggcgc cgcgccgcaa
       61 ccggagcagc atctgcaaag cctcggctcc ggcttcatca tcgatccggc ggggctggtc
      121 gtcaccaaca accatgtcat cgagggcgcc gaccagatca ccgtcaccct gcaggacggc
      181 acgacgctga aagcctcggt ggtcgggcgg gacgatgtcg gcgacatcgc tctgctgcgg
      241 gtcaagccga ccaagccgct gccttcggtc agtttcggcg attcggccaa ggagcgggtg
      301 ggcgactggg ttctggcgat cggcaatccg ttcggcctgg gcggcacggt caccgccggg
      361 atcatctcgg cgcgcgggcg caacatccat caggggccgt acgacgactt cctgcagacc
      421 gacgcgccga tcaaccaagg caattccggc ggcccgcttt tcaacatgga cggccaggtc
      481 atcgggatga acacggcgat ctattcgcgt tccggcggat cggtcggcat cggcttctcg
      541 atcccgtcca acatggtgaa ggacgtcgtt gagcaactgc atcaatatgg ccatccgcgc
      601 cgcggctggc tcggcgtgcg catccagcaa gtcacgccgg atattgcgga gagcctgggg
      661 ctgaagcagc cgaggggcgc gctggtggcc ggggtgaatg atggcgggcc ggcggacaag
      721 gcgaagctgc acaacggcga cgtgatcctg aagttcaaca atgaggacgt gaaggacatg
      781 catgccctgc cgcgcatcgt cgcggaggcg tcggtgggca agacggtgcc ggtggtggtg
      841 tggcgtgacg gcaaggacgt aacgctgcag gcgacgctgg cggaaaagcc ggacgatata
      901 aagacagcca cggcccccgc gccgaagccg gcgctggacc agccgaccgc gattgccgga
      961 ctgggcatga agctggcgcc gctgtccccg gcgctgcggc agaaatatca gctcggtgcg
     1021 gaccagaagg gcgtggtggt cacggatgtc gagccgaagg ggccggccgc ggagcgcggc
     1081 ctcaagccgg gcgacgtgat cgtggaggtg cagcagggcg aggtcggtac gcccgccgag
     1141 gtgcagcagc gcatcgaggg cgtgcgcaag gagaaccgca agtcggtgct gatgctgatc
     1201 cagcggcagg atggcctgca atgggtgccg ctgccgctgt ccggtgggcc ggaaaagcag
     1261 ccaggctgac cctgcgggct cgccaccgaa accggcagat ttgattccat aatccgttaa
     1321 gccgccgtta tcctgcggca aatgctcgac gacgacgatt acgatcgcct ggtcgctcaa
     1381 atttacgcgg cagccgcgga tgcttctctg cttcccgcat tgctcgagga cctgcgtgcg
     1441 ggtttcggcg gctccgctgc cgccctgatc gccttctccg aagcgccggg ccagagtcgg
     1501 gttgtggcga cgcgccagga agccatggcc gagtaccggc gccactggga cgcgcgcaat
     1561 ccgctgcacc cgttacgccg caccggcggc caactggcgc ccggcacgat cgccaccgac
     1621 gcgatgctga tgccgcggga cgcgctgatc cggacggatt acttcaacga gttcctgctg
     1681 gcctacgagc tgccgaacct gcttgcggtg aagctcgcgg cgggcgtcgg tcaggatgtc
     1741 acgctgaacc tgtttcgtgg cacccgccag ggcgggtttg atccggtcca tctcaaggtg
     1801 gcgcgccgcc ttggcccgca tctgcatggg gcgacgcgga tcgcctcccg gctggcctgg
     1861 gctggggtca aggcgggggg gagtgatgcc gcgctggacc ggctggtgag cggcaccgtg
     1921 ctgctcgacc gcagcggcag agtggtgcac gtcaatgcgg ccgccgaacg catgctggcg
     1981 gagcaggacg ggctgcaact ggccaatcgc cggctcactg cgaaatcgcc gggcgaacat
     2041 gccgcgctgg cgcggttgat cggccgggca gccggggagg gcgatgcctg cggctgcggt
     2101 gggatcaccg tgtcccggcc gtccgggcgg cgtgcctggg ccctgattgc ggcgcctttg
     2161 cgggtggagg tggcgtggct cgccccgcaa cggcccgcgg tggtggtcag catcaccgat
     2221 ccggaggtca cgcccacgcc cgcggcggat cgcctggtcc agctctacgg cctcacagcg
     2281 cgcgaggccg acgttgccct gggcatcgcg cgtggcgcgg aactgcggga agtcgccgac
     2341 gggctcggcc tgacgcaact gtcggcgcgg caatacctca gccgggcgct gcgcaagacc
     2401 ggcgcacacc ggcagcacga cctgacccgg ctgctggtca gcctcggggc ggcggccggt
     2461 tgagcggttc ttgctggtgc ggaaattttt tggattcgtt cacacctgtg aatatccgga
     2521 tcagtccgac ccgccgagta tcgcctcaca gcaccgggtc tcagccggtg caggagggga
     2581 gaatttccgg atgcgcgtgt ttcgcctgat tggccttcta ggcatcgccg ccagcctgtc
     2641 ggggccggct tgggcgacaa cttacgacgc ggtgaccgat ttcaacgcga ccagcaacgc
     2701 tggcggaaat atctggacct accgctccgg caccacgggg agcgggacgc tgttgaccgg
     2761 caccagcaca acgagccccg gctctctgcc ccaatgggat ggccctgggt atccctatat
     2821 ttccgccaat aattcggcga ccctttatca aggcggcaca gttctctacg agccgaacgc
     2881 gttgcatctg gacggcggtg gcgtgggcgc cg
//
LOCUS       DASSZH010000673         5002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1124383, whole genome shotgun sequence.
ACCESSION   DASSZH010000673 DASSZH010000000
VERSION     DASSZH010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124383"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VFN42_14535"
     CDS             <1..594
                     /locus_tag="VFN42_14535"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8997883.1"
                     /translation="LSDMETLDDRAALVVEQHGPIDILVNNAGVTVLGSVLETRLAEV
                     ERVMRVNFLAAYGMMRAVLPGMLARRRGAIVNIASDQALIGKPLSAAYGASKAALAQL
                     TRSAALDWAAGGVRVNCIAPGSTDTPMLNAVIGELATRYPDRFPNSSVEAYRAGVPLG
                     RFARPEEIAAVAAFLASDAASFITGVVLPVDGGTTAA"
     gene            591..1289
                     /locus_tag="VFN42_14540"
     CDS             591..1289
                     /locus_tag="VFN42_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8997884.1"
                     /translation="MTARLALVTGGTRGIGRAIAARLAAEGCRVIVAARTAPETAEFE
                     FRRCDVTDPASVAALFAALDRLDVLVNNAGLAGANRLDADDALWHAIIASNLHGTYHC
                     CKAALPLLPDGTGRIVNIASVLGLRGVPDQTAYCAAKHGVVGFTRALALALAPRGITV
                     NALCPGWVDTGMAQRRFAELGITAEQAAQGVPTGRIVTPEEVAAAVAWLIRPEAGSVT
                     GLALPLDGGALAGP"
     gene            1534..4038
                     /locus_tag="VFN42_14545"
     CDS             1534..4038
                     /locus_tag="VFN42_14545"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HET8997885.1"
                     /translation="MMRVRLLLIGLILLLPMAAVAQPSAALSPEQARTALEVLNNPRK
                     RAEVASTLEAIAAAKPDAAPAQPAATDKAPPPAAKPGPAPAAGTAAPAQPASSTEALK
                     IPLAPDSLGAQVLVSASDFLTRAGRQTMDALHAVQSVPLLWGWMVVMATNPWARSMLD
                     DTVWRLLVVMACALAVEYALRRMIARPITALERMAPGGTVQPEPSEPEILPPDVADPL
                     LEPDEAPDEPPEDAEARAEAGDTEPSPHPQRRRVSNWTQLRRIPLVFARLLLDLIPVL
                     GFVVTGHLLSGSALGGQAISRLVLLAVVDSYAVCIILLSVARMLLSPARARLRLFNLR
                     DDIASYLMRWTRRLVVIGVFGYAVAEVGLLLGLSDVAHEAVLKTVSLALHIGLAIIVV
                     QKRRAVRARIRAPAGETGFAARLRNGLAASWHWIALFFIIGVWVVWAVEVPHGFAWFL
                     RIFVITALVLIGARLTLIVLLGSIDRALRIAPEVSERYPGLEARVRLYAPMIHGTVRF
                     LLFTLCFLGLLQLYGLPTFTWFAGSALGQRVVSACGTMFVTVLIALAVWEAANAAIHR
                     HLAKLQKEAQIARSARLRTLLPLLRSGLMISILTVAGLMLLSEIGLNIAPLLAGAGII
                     GVAIGFGSQKLVQDLITGIFLLLENAMQVGDVVNVAGHAGVVESLSVRTIRLRSEDGS
                     VHVIPFSSVTTVTNMTKDYSRAVISAGVAYKEDYDGVVEVLRTIAKEMRGEPQWKTVI
                     LDDLEVWGLDQLGDSSVVIKCRIMCTPFGRWSVQREFNRRMKKRFDELGIEIPFPHRK
                     LVLEGVLPLQAAPPAQLPPPAAEAATAVTLPTMRPA"
     gene            4035..4757
                     /locus_tag="VFN42_14550"
     CDS             4035..4757
                     /locus_tag="VFN42_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011253196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe dioxygenase family protein"
                     /protein_id="HET8997886.1"
                     /translation="MSGPDMDALAAAMLADGFALVRAPAMRGLLERFGSLADWGAFAA
                     SWNDLGLDTYMADGGRYRKRRHAAFGVNAGEAEIVRKPHQPHYQSRDYNPLNGGVERW
                     FSPIAPEIGAGASMTAVLRTCRDLFDRLTPAPAWHIEVHQFRIEARRGEAGRPTPEGM
                     HRDGVDHVLVLLIGRQNVQSGETSIHDLEGRTLGSFTLTEPLDAALVDDRRVMHGVTP
                     VEPVDPDQPGFRDVLVVTFRRG"
     gene            4840..>5002
                     /locus_tag="VFN42_14555"
     CDS             4840..>5002
                     /locus_tag="VFN42_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8997887.1"
                     /translation="MPEWSKTYDVLVAGGGNAALCAAITARQAGASVIVLESAPKALR
                     GGNSRHTRNL"
BASE COUNT          736 a         1718 c         1752 g          796 t
ORIGIN      
        1 ctctccgaca tggaaaccct cgacgatcgg gccgccttgg tggtcgagca gcatggccct
       61 atcgacatcc tggtgaacaa tgccggcgtt accgtgctcg gcagcgtgct ggagacgcgg
      121 ttggccgagg tggaacgggt gatgcgggtg aacttcctcg ccgcctatgg gatgatgcgc
      181 gccgtgctgc ccggcatgct ggcgcggcgg cggggcgcga ttgtcaatat cgcctccgac
      241 caggcgctga tcggcaaacc gctgagcgcg gcgtacggcg ccagcaaggc cgcactggcc
      301 cagcttaccc gcagcgccgc gctggactgg gccgctgggg gggtgcgggt caattgcatc
      361 gcgccgggct ccaccgatac gccgatgctg aacgcggtga tcggcgaact cgccaccaga
      421 tatccggacc ggttccccaa ctcatcggtc gaggcgtatc gcgccggcgt accactgggc
      481 cgcttcgcac gcccggagga aatcgccgcc gtggcggctt tcctggcctc ggatgccgca
      541 tccttcatca ccggcgtggt gctgccggtc gatggtggca ccacggccgc atgaccgccc
      601 ggctggcact ggtcacgggc ggaacacggg gcatcggccg ggcgattgcc gcacgcctgg
      661 ccgccgaggg ttgccgcgtc atcgtggccg cgcggaccgc accggaaacg gccgagttcg
      721 agttccgccg ctgcgacgtg accgatcccg cctcggttgc cgcgctgttc gccgccctgg
      781 accgcctgga tgtgctggtg aacaacgccg gccttgccgg cgccaaccgc ctggatgcgg
      841 acgacgcgct gtggcacgcc atcatcgcca gcaacctgca tggcacctat cactgctgca
      901 aggcggcgct gccgctgctg cccgacggca cgggccggat cgtcaacatc gcctcggtgc
      961 tgggattgcg cggggtgccg gaccagaccg cctattgcgc cgcgaagcac ggggtggtcg
     1021 gtttcacccg cgccctggcg ctggccctgg caccacgcgg catcacggtg aacgcgctct
     1081 gcccggggtg ggtggacacc ggcatggccc agcggcgttt tgccgagctg gggatcaccg
     1141 cggaacaggc cgcccaaggc gtccccaccg gccgcatcgt cacgccggag gaggttgccg
     1201 ccgccgtcgc atggctgatc cgccccgaag cgggcagcgt gacgggtctc gccctgcctc
     1261 tggacggcgg agcgctggcg gggccctgag ccggcgaaaa ccccgcccgg cgtccatagc
     1321 agttgacccg ggcgcgcggt ttatggtcgg acccgccgca tgataccagc ggccaaaaat
     1381 tccgacccct ccgtgtcatg cacgggcttg gcccgtgcat ccacgtcttg ctgtggtgca
     1441 cgcagcagag gcatgggtgg ccggatcaag cccggccatg ccacggggga ggagacgctc
     1501 cgccggaccg gcgcagaacg atgggccgcg ggtatgatgc gcgtccggct gctcctgatc
     1561 ggcttgatcc tgctgctgcc catggccgca gtggcccagc cgtccgcggc cctgagcccc
     1621 gaacaggcac gcactgcgct ggaagtgctg aacaatccca ggaaacgggc ggaagttgcc
     1681 agcaccctgg aggcaattgc cgcggccaag cccgacgcgg cgcccgccca gccggccgcc
     1741 acggacaagg cgccaccgcc cgcggccaag ccgggtcctg cgccggcggc aggaacggca
     1801 gcgcctgccc agccggccag ctccaccgag gcactgaaaa tcccgctggc gccggatagc
     1861 ctgggcgccc aggtgctggt cagcgcctcc gacttcctga cccgcgccgg ccggcagacc
     1921 atggacgccc tgcacgccgt ccagagcgtg ccgctgctct ggggctggat ggtggtgatg
     1981 gccaccaatc cctgggcgcg cagcatgctg gacgacacgg tctggcgcct gctggtggtg
     2041 atggcctgcg cactggcggt ggaatatgcc ctgcgccgga tgattgcccg gccaatcacc
     2101 gccctggaac ggatggcccc gggcgggacg gttcagccgg agccatcgga acccgagatc
     2161 ctgccgccgg acgtcgctga cccgctgctg gagccggatg aggcgccgga cgaaccgccg
     2221 gaagatgccg aggcgcgcgc ggaggccggc gataccgaac cctcgccgca tccccagcgc
     2281 cgccgcgtgt ccaactggac ccaactccgc cgcatcccgc tggtgttcgc ccgcctgctg
     2341 ctggacctga tcccggtgct cggctttgtg gtcaccggcc acctgctcag cggcagcgcc
     2401 ctgggtggcc aggcgatcag ccgcctggtg ctgctggccg tggtcgattc ctatgcggtg
     2461 tgcatcatcc tgctcagcgt ggcgcggatg ctgctgtccc ccgcacgcgc gcggctgcgg
     2521 ctgttcaacc tgcgcgacga tattgccagc tacctgatgc gctggacccg ccgtctcgtg
     2581 gtgatcggcg tgttcggcta cgcggtggcc gaggtcgggc tcctgctcgg cttgtccgac
     2641 gtggcgcatg aagccgtgct gaagacggtc agcctggcgc tgcatatcgg cctggccatc
     2701 atcgtggtgc agaagcggcg agcggtgcgc gccagaatcc gcgccccggc aggcgaaacc
     2761 ggatttgccg cgcggctgcg caacggcctg gcggcttcct ggcactggat cgcgctgttc
     2821 ttcatcatcg gtgtctgggt ggtctgggcg gtggaggtgc cgcacggctt cgcctggttc
     2881 ctgcggatct tcgtgatcac cgcgctggtg ctgatcggcg cgcgcctgac gctgatcgtc
     2941 ctgcttggct cgatcgaccg ggcgctgcgg atcgcaccgg aggtgtcgga acgctatccc
     3001 ggcctggaag cgcgggtgcg cctgtacgcg ccgatgatcc acggcaccgt ccgtttcctg
     3061 ctgttcacgc tctgcttcct cggcctgctg cagctctatg gcttgccgac cttcacctgg
     3121 ttcgccggca gcgcgcttgg tcagcgcgtc gtctccgcct gcggcaccat gttcgtcacc
     3181 gtgctgatcg ccctcgcggt atgggaggcg gcgaacgccg ccatccaccg ccacctggcc
     3241 aagctgcaga aggaagcgca gatcgcccgc tccgcccggc tgcgcacgct gctgccactg
     3301 ctgcgcagcg ggctgatgat cagcatcctc accgtcgcgg gattgatgct gctgagcgag
     3361 atcggcctga acatcgcgcc gctgctggcc ggcgcgggca tcatcggcgt ggcgatcggc
     3421 ttcggctcgc agaagctggt gcaggacctg atcaccggca ttttcctgct gctggagaac
     3481 gccatgcagg tcggcgatgt cgtcaatgtc gccgggcacg ccggcgtggt ggaaagcctg
     3541 tcggtgcgca ccatccggct gcgctccgag gacggatcgg tgcacgtgat ccccttcagt
     3601 tcggtgacca cggtcaccaa catgaccaag gattacagcc gcgcggtgat cagcgccggc
     3661 gtggcttaca aggaggatta cgacggcgtc gtggaggtac tcaggacgat cgccaaggaa
     3721 atgcgcggcg aaccgcaatg gaaaaccgtg atcttggacg acctggaagt gtggggattg
     3781 gaccaactcg gcgactcgtc ggttgtgatc aaatgccgga tcatgtgcac ccccttcggc
     3841 cgctggtcgg tccaacgtga gttcaatcgc cggatgaaga agcgcttcga cgagctgggc
     3901 atcgagatac cgttcccgca ccgcaagctg gtgctggagg gcgtgctgcc gctgcaggct
     3961 gcgccgccgg cgcagctgcc gccgccggcc gccgaagcgg caaccgccgt cacgcttccc
     4021 accatgcgcc cggcatgagc ggcccggata tggacgcact ggccgccgcc atgctggcgg
     4081 atggctttgc cctggtgcgg gcgccggcca tgcgcgggtt gctggagcgg ttcggctcac
     4141 tggcggactg gggcgcgttt gccgcaagct ggaacgacct cggcctggac acctacatgg
     4201 cggatggcgg ccgctatcga aaacggcgcc atgcggcgtt cggcgtgaat gccggcgaag
     4261 cggagatcgt ccgcaagccg caccagccgc actatcagag ccgggactac aacccgctga
     4321 acggtggggt cgaacgctgg ttctctccga tagcgccgga gatcggcgcc ggcgccagca
     4381 tgaccgcggt gctgcgcacc tgccgcgacc tgttcgatcg gctgactccg gcaccggcct
     4441 ggcatatcga ggtgcatcag ttccgcatcg aggcgcgccg cggtgaagcc ggccgcccaa
     4501 ctccggaggg catgcatcgc gacggcgtgg atcacgtgct ggtgctgctg atcggccggc
     4561 agaacgtgca gagcggcgaa acctccatcc acgacctgga aggccgcacg ctgggcagtt
     4621 ttacgctgac cgagccgctg gatgcggcgc tggtggacga ccggcgggtg atgcacgggg
     4681 tgacgccggt cgagccggtc gatccggacc agcctggctt ccgcgacgtg ctggtggtga
     4741 cgttccggcg ggggtgagct tcgccggagt ggcgaacgga cctgccgccc tttccacccc
     4801 ggtccgaatc ggctctaaaa gtccccgcgg aggggaaaca tgcccgagtg gagcaagacc
     4861 tacgacgtgc tggtcgcggg gggcggcaat gcggccctgt gcgcggcgat caccgcgcgc
     4921 caggccggtg ccagcgtcat cgtgctggaa tccgcgccga aggcgttgcg cggcggcaac
     4981 agccgccaca cgcgcaacct gc
//
LOCUS       DASSZH010000674         2365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_868692, whole genome shotgun sequence.
ACCESSION   DASSZH010000674 DASSZH010000000
VERSION     DASSZH010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868692"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..1232
                     /locus_tag="VFN42_14560"
     CDS             66..1232
                     /locus_tag="VFN42_14560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8997888.1"
                     /translation="MRSWYFSEMAYHPAWDEGLKRGSLRVVLPNENVDPHTAHKLLHR
                     YLDEFALCDEVGLDIMVNEHHSTATCMTVSVPMALAVIARETKRARLLTLGNPIANRP
                     DPVRVAEEMAWLDCLSGGRLEMGLVKGAPYEIAPANSNPARLMRRYWEAHDLILKALS
                     TTDGPFNWEGEYFHYRNVNIWPRPIQQPAPPVWMTGMSVESGRLAAERRHVIGTLLSA
                     GTAKPMYDMYRKTAQANGWEAGPDRFAYAAVVGVGSTREEGLRRADQIADYVRTAPVV
                     AEPFTNPPGYNSVAANVAMLKAGPRAHRIVKDRQGNTIDHRKATVQQFMDTDTVFAGT
                     PDDVYAQVKDFNARMGGVGHLLFFGQGGFLNHQDAMENIRLFGHEVAPRLRELN"
     gene            complement(1313..>2365)
                     /locus_tag="VFN42_14565"
     CDS             complement(1313..>2365)
                     /locus_tag="VFN42_14565"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HET8997889.1"
                     /translation="APANPVDVTAQGSNTGPAVMTAMEQLAESDEVDMLVLISSMAST
                     TRATLDAARVRAVAESSGKPMTVWTYTLPSAFGRASAAGCGLFVHSDLRDVGVSLAKL
                     ADYAEALASPLPADPQPRDVAPLPPGAAGVLPEYHAKRALAAFLPAMKERLATTAAEA
                     ADAAAALGFPVALKAQSPDLPHKTEAGGVRLGLSDAAAVRTAYDAMLRDVLQHRPDAR
                     IDGVLVQKMAPKGHELVIGMVNDPTFGPIMMLGFGGTTVELFSDVVHSPAPVDAAEAR
                     RMLLSLKSAPLLTGFRGAAKVDLAPVATLVAQLSEAALAYRDQIAEMEFNPVILHADG
                     SGLTIADALVRLKGAA"
BASE COUNT          412 a          783 c          799 g          371 t
ORIGIN      
        1 caaccgcaac agttcgccga gcgggtcgcc gcttttgcgc aacagcactg acggggaaaa
       61 aggcgatgcg cagctggtat ttctccgaga tggcgtatca cccggcgtgg gacgaagggc
      121 tgaagcgagg ctcgctccgc gtggtgctgc caaacgagaa tgtcgatccg cataccgcgc
      181 acaagctgct gcaccgctat ctggatgagt tcgcgctgtg cgacgaggtc gggctggaca
      241 tcatggtgaa cgagcaccac agcaccgcca cctgcatgac ggtgtcggtg ccgatggcgc
      301 tggcggtgat tgcgcgggaa accaagcgtg cgcgcctgct gacgctgggc aatccgatcg
      361 ccaaccggcc cgatccggtg cgggtggcgg aggaaatggc gtggctggac tgcctgtccg
      421 gtggaaggct ggagatgggt ctggtgaaag gcgcgcccta cgagatcgcg cccgccaact
      481 ccaatcccgc gcggctgatg cggcgctact gggaggcgca tgacctgatc ctgaaggcgc
      541 tgtccaccac cgacgggccg ttcaactggg aaggcgagta cttccactac cgcaacgtga
      601 acatctggcc gcggccgatc cagcaaccgg cgccgccggt gtggatgacc ggcatgagcg
      661 tggagagcgg ccggctggcg gcggaacggc ggcacgtcat cggcacgctg ctgtccgctg
      721 gcacggcgaa gccgatgtac gacatgtatc gcaaaacggc gcaggcgaac gggtgggagg
      781 ccggcccgga ccgcttcgcc tatgccgcgg tggtcggcgt gggaagcacg cgcgaggaag
      841 ggctgcggcg ggcggaccag atcgcggatt atgtgcgcac cgcacctgtg gtggcggagc
      901 cgttcaccaa cccgccgggc tacaattcgg tcgcggcgaa cgtggcgatg ctgaaggcgg
      961 gaccgcgcgc gcatcgcatc gtcaaggacc gccagggaaa cacgatcgac caccgcaagg
     1021 cgacagtgca gcagttcatg gacaccgaca cggtgtttgc cggcacgccg gacgacgtgt
     1081 acgcgcaggt gaaggatttc aatgcgcgca tgggcggcgt gggccacctg ctgttcttcg
     1141 gccagggcgg cttcctgaac caccaggacg cgatggaaaa catccggttg ttcgggcacg
     1201 aggtggcgcc gcggctgcgg gaactgaact gaaacattcc tccccctccc cttgcgggag
     1261 ggggaggagt ctcctcatgg cttgtcctga tacttgtgca gactatgccg cgctacgccg
     1321 cgcccttcag cctcaccaaa gcatcggcga tcgtcaggcc ggatccgtcg gcgtgcagga
     1381 tcaccgggtt gaactccatc tcggcgatct gatcccggta cgccagtgcc gcctcggaca
     1441 gttgcgcgac caaggtcgcg accggcgcca ggtccacctt cgccgccccg cggaaacccg
     1501 tgagcagcgg cgcggacttc agtgacagca gcatgcggcg cgcttccgcg gcatccactg
     1561 gcgccgggct gtgcaccacg tcgctgaaca gttccaccgt ggtgccgccg aacccgagca
     1621 tcatgatcgg accaaacgtc ggatcgttga ccatgccgat gaccagttca tggcccttgg
     1681 gcgccatctt ctgcaccagc acgccgtcga tacgggcatc tggccggtgt tgcagcacgt
     1741 cgcgcagcat cgcgtcgtac gctgtccgca ccgcggccgc atccgacaaa cccagccgca
     1801 cgccaccggc ttcggtcttg tgcggcagat cgggtgactg cgccttcaac gccaccggga
     1861 aaccaagagc ggctgcggca tcggctgctt cggccgcggt ggtcgccagc cgctccttca
     1921 tggctggcag gaacgccgcc agcgcacgct tggcatgata ttcgggcagc acgcccgccg
     1981 cgccgggcgg cagtggcgca acgtcgcgcg gttgtggatc ggccggcaga ggactcgcca
     2041 gcgcctcggc ataatccgcg agtttcgcca gtgacacgcc gacatcgcgc aggtcgctgt
     2101 gcacgaacag cccgcatccc gccgccgacg cgcggccaaa tgcgctcggc agagtgtagg
     2161 tccacaccgt catcggcttg ccggagcttt cagcgacagc cctcacccgc gcggcatcca
     2221 gcgtcgcgcg tgtggtgctg gccatcgagc tgatcagcac cagcatgtcc acttcgtcgg
     2281 attcggcgag ctgctccatc gccgtcatca cggcaggccc ggtgttcgat ccctgcgcgg
     2341 tgacatccac cggattggcc ggcgc
//
LOCUS       DASSZH010000675         3170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1243881, whole genome shotgun sequence.
ACCESSION   DASSZH010000675 DASSZH010000000
VERSION     DASSZH010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243881"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VFN42_14570"
     CDS             complement(<1..373)
                     /locus_tag="VFN42_14570"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8997890.1"
                     /translation="MAALGRRARAATPGVTATEIKIGNTMPYSGPASSYGAIGKAETA
                     FFKMVNAQGGVAGRKIDFISLDDGYLPPRTVEDVRRLIEEDQVAFLFNTLGTPTNSAI
                     ERYVNHKKVPHLFLATGADKWG"
     gene            739..1050
                     /locus_tag="VFN42_14575"
     CDS             739..1050
                     /locus_tag="VFN42_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HET8997891.1"
                     /translation="MSAPSLDDQTRTELEAAAFRRLVEHLRERTDVQNIDMMNLAGFC
                     RNCLSRWYREAADAKGLGLSDPDAREIVYGMPYKEWQAKYQKEASAEQKAAFAKAQPG
                     H"
     gene            complement(1126..1560)
                     /locus_tag="VFN42_14580"
     CDS             complement(1126..1560)
                     /locus_tag="VFN42_14580"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET8997892.1"
                     /translation="MLVSDVLRSKGRHVVKVRTTDPVALAVGKLAENRIGALVVEDQW
                     MHLVGIFSERDFVNAVARDGAAVLALDVQQLMSSPIIGCGSGDRIDAALATMTLAKIR
                     HLPVIDDGVLKGIVSIGDLVKHRLDEKELEANVLLELSRMRA"
     gene            1786..2685
                     /locus_tag="VFN42_14585"
     CDS             1786..2685
                     /locus_tag="VFN42_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET8997893.1"
                     /translation="MIDKLEMLLALAREQHFGRAAEACGVTQPTLSAGLKQLEETLGV
                     LLVNRQSRFIGFTPEGLRVLDWARRIVADSRAMREEIRALRRGLAGHLRIAAIPTALP
                     IVAELTTPLRARHPDVLFSVLSRTSIDTLTQLENLEVDAGLTYVDNEPLGRVRSIALY
                     EERYRLLTAAEAPLGDRDEVTWGEVGKIPLCLLTPDMQNRRIIDRRLREAGTEPAPTL
                     QSNSMVVLFSHVRTGRWASVMPAVLAETMGITQPIRSIPIAEDGPPPMVGLVYPQREP
                     MTPLTAALVTEARRLAQRWRGAG"
     gene            2772..>3170
                     /locus_tag="VFN42_14590"
     CDS             2772..>3170
                     /locus_tag="VFN42_14590"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET8997894.1"
                     /translation="MDLKEHIRGIPDFPKPGVLFYDISTLLRDADAWQVAMGRMAKAV
                     RAYQPDLLAGLESRGFLIAAPLALKLGCGFIMLRKRNKLPGETVGLNYSLEYGTDRLE
                     VQADAVEPGQRVVVVDDLLATGGTMAAGIGL"
BASE COUNT          510 a         1061 c         1072 g          527 t
ORIGIN      
        1 cgccccattt gtccgcgccg gttgcgagaa acaggtgcgg cactttcttg tggttgacgt
       61 agcgttcgat cgccgagttg gtcggcgtgc cgagcgtatt gaacaggaaa gcgacctggt
      121 cttcctcgat cagccggcgc acgtcttcca ccgtgcgcgg cggcaggtag ccgtcgtcga
      181 gcgagatgaa gtcgatcttg cgcccggcca cgccgccctg cgcgttgacc attttgaaga
      241 acgcggtttc agccttgccg atcgctccat aggaggaagc ggggccgctg tacggcatgg
      301 tattgccgat cttgatttca gtggcggtta cacccggtgt ggcggcccgg gctcgcctgc
      361 ccagcgctgc catgcccacc gcggttccgc ccgcgagggc ggcacgtctg gttatcatcc
      421 cgtatcatct cccttggaat tgcccgcctg catgccatgc cgatcaggct ggtttggcgg
      481 cgcgaggata tcgaacccgt aactgatcag caagcattgg tacagccggt tgacgccgtg
      541 ttttcacgtg caaccgctgc ggcagggatg gaaagcgcag gccggttgcg tgatcccggc
      601 gacatgccac ggcccgatgg ctgcgcttgc ctggcaggtc gggatgcggg agagtcggtg
      661 gttatggcgc gcgttggcca ctctctgctc cggccatagc gtcgccctcc gccacgccag
      721 ccgcaaagga ggtccgcgat gtccgcaccc agtctcgacg accagactcg caccgaattg
      781 gaagccgccg ccttccgccg cctggtcgag catctgcgcg agcgcaccga cgtgcagaac
      841 atcgacatga tgaatctcgc cggattttgc cgcaactgtc tcagccgctg gtatcgcgaa
      901 gctgccgatg cgaaaggtct cggcctgagc gatccggatg cgcgcgagat cgtctatggc
      961 atgccgtaca aggaatggca ggccaagtac cagaaggaag ccagcgccga gcagaaggcg
     1021 gcgtttgcca aggcgcagcc gggtcactga tcgtcgcggc gtgaatgaaa gccgccccgt
     1081 cgccgccggc ctggcatcgg ccggcatgac ggggcggtgc cggcatcagg cgcgcatgcg
     1141 cgacagctcc agcagcacgt tggcttccag ctccttctca tccagccggt gcttcaccag
     1201 gtcgccgatg ctgacgatgc ccttcagcac cccatcatcg atcaccggca ggtgccggat
     1261 cttggccagc gtcatggttg ccagcgccgc gtcgatacgg tcgccggagc cgcagccgat
     1321 gatgggcgat gacatgagct gctggacatc cagggcaagc accgcggcgc cgtcgcgggc
     1381 cacggcattg acgaagtcac gctcggagaa gatccccacg aggtgcatcc actggtcttc
     1441 caccaccagg gcgccgatcc ggttctcggc cagcttgccc acggccagcg cgacaggatc
     1501 ggtcgtgcgc accttgacca cgtggcggcc cttgctccgc agaacgtcgg aaacgagcat
     1561 tgtcatgtcc tcctgaccca gcggttccgg ggtctgtccg ggaggcaaca gtgcgacggg
     1621 ggcagacccc ttgcatgacg gcacatgggc agcggccggg caggcgccaa gcggtggcgc
     1681 aatgaggctt tgtccatccg ccggcgctgc acttgccgcc agcttgcgcg tagaacacgg
     1741 catgcagacc acgcgtgtgc ccggcaatgc caccggggtg tccggatgat cgacaagctg
     1801 gagatgctgc tggcgctggc ccgcgagcag catttcggcc gcgcggccga ggcctgcggc
     1861 gtcacccagc ccacgctgtc cgccggactg aagcagttgg aggagaccct gggcgtgttg
     1921 ctggtgaacc gccagtcgcg cttcatcggc ttcacgccgg aagggctgcg cgtgctggac
     1981 tgggcacgcc ggatcgtcgc cgactcccgc gccatgcggg aggagatccg cgcactgcgc
     2041 cgcggcctgg ccggccatct gcgcatcgcc gccattccca ccgcgctgcc gatcgtcgcc
     2101 gaactcacca cgccgttgcg cgcgcgccat ccggacgtgc tgttcagcgt gctgtcccgc
     2161 acctcgatcg acaccctgac ccagttggaa aacctggagg tggatgccgg gctgacctac
     2221 gtcgacaacg agccgctggg ccgggtgcgc tccatcgcct tgtatgagga acgctaccgg
     2281 ttgctgaccg cggccgaagc cccgctgggc gaccgcgacg aggtcacctg gggcgaggtg
     2341 gggaagatcc cgctgtgcct gctgacaccc gatatgcaga accgccgcat catcgaccgc
     2401 cggctgcgcg aagcgggcac cgaaccggcg cccaccttgc agtcgaactc catggtggtg
     2461 ctgttctccc atgtgcgcac cgggcgctgg gccagcgtga tgccggcggt actggcggag
     2521 acgatgggga tcacccagcc gatccgttcc atcccgatcg cggaggacgg gccgccgccg
     2581 atggtcggcc tggtgtatcc ccagcgtgaa ccgatgacgc cgttgaccgc cgcgctggtc
     2641 accgaagccc ggcggctggc gcagcgctgg cgcggggcgg gatgaaagcg ccctgcgctc
     2701 gagcctcgtt gcggcccccg gcctggagct ttacacccgc ctcagcgata ggcgggaagg
     2761 ggtcggccat catggatctg aaggagcaca tccgcggcat tcccgatttc cccaagccgg
     2821 gggtgctgtt ctatgacatc tccaccctgt tgcgggatgc cgatgcctgg caggtggcca
     2881 tgggccgcat ggcgaaggca gtgcgcgcct accagccgga cctgctcgcc ggcctggaaa
     2941 gccgcggctt cctgatcgcc gccccgctcg ccctgaagct cggctgcggc ttcatcatgc
     3001 tgcggaagcg gaacaagctg ccgggcgaga cggtggggct gaactactcg ctggaatacg
     3061 gcaccgaccg gttggaggtg caggccgacg cggtggaacc cggccagcgc gtggtggtgg
     3121 tggacgacct gctcgcaacc ggcggcacca tggcggcggg tatcggcctg
//
LOCUS       DASSZH010000676         3072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_664406, whole genome shotgun sequence.
ACCESSION   DASSZH010000676 DASSZH010000000
VERSION     DASSZH010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664406"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VFN42_14595"
     CDS             <1..200
                     /locus_tag="VFN42_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997895.1"
                     /translation="PAKSLGGAMRVEWAFFDGPTKFMVSLSTAGQPGPIRLQMRLHDT
                     EWRVDRVWLPPHLLDQQDART"
     gene            complement(212..1552)
                     /locus_tag="VFN42_14600"
     CDS             complement(212..1552)
                     /locus_tag="VFN42_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013641013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET8997896.1"
                     /translation="MRTIEQLAQGLEQDHTTSRALVEDSLARIADPGGEGGRAFIRVY
                     AASARAMADAMDTLRRAGRAPTRWAGIPIGLKDLFDVAGEPTPAGSRVLADAPPAASH
                     APVVQRMLAAGFVPMGRTNMTEFAFSGLGINPHYGTPLSPWDRAGRRIPGGSSSGTAV
                     AVADGMVAAGLGTDTGGSCRIPAAFCGIVGYKPTARRVPIDGVLPLAPSLDSVGPLAP
                     SVACCAVIDAVLAGTPPVAPALAALAGLRLAVPENMVLEGTDTHVATAFDRVLAALSA
                     AGARIERRRFAALDALPAVNAKGGFAASEAYAWHRALLARAGAGYDPRIRLRIERGAQ
                     MDAADYLDLTAARARLIAEFNAETQSFDALVMPTSPIVPPRLDELEDERDYNRQNLLI
                     LRNTALGNFLDRCAISLPCHREGEAPAGLMLMGETLGDARLFGIAAAVEQIMRG"
     gene            1587..2417
                     /locus_tag="VFN42_14605"
     CDS             1587..2417
                     /locus_tag="VFN42_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8997897.1"
                     /translation="MTDKPAKPVLLTGASGALGRVLTKSLAAEGWTLRLTDIAPFPDP
                     IPAGADFTRADLNDGVTMLRLAEGCGAIIHLGGVSVERPFEEVLGPNIRGLYHIYEAA
                     RRESARVIFASSNHSIGFHERSESLPAGAQFLPDGFYGLSKAYGELMGRLYWFKHGVE
                     SVFIRIGSAFPEPVDARMLATWLSYPDLSRLMIRCVVSEKVGCCVVWGASNNSRMTWW
                     RDDARDALGWAPQDSADPFAGQLAGKVSSDPVQERYMGGGFCAIGYSRSAPAPAELFD
                     "
     gene            complement(2483..>3072)
                     /locus_tag="VFN42_14610"
     CDS             complement(2483..>3072)
                     /locus_tag="VFN42_14610"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HET8997898.1"
                     /translation="AGVRHVVALRGDPPPGSEYAPHPGGYAFAADLVAGLRRVADFEI
                     SVAAYPETHPSALSPDADLDNLKRKLDAGATRAITQFFFDTGVFLRFLDRALAAGITA
                     PIVPGIMPVTNLAGLRRMAALCGSAVPDWLGHMFEGTDDDPEIRRMVAAVVTAEQVRL
                     LQANGVDEFHFYTLNRPELTYAIAHILGVRPRAAG"
BASE COUNT          476 a         1057 c         1067 g          472 t
ORIGIN      
        1 ggccggccaa aagcctcggc ggcgcgatgc gggtggagtg ggcgttcttc gacggcccga
       61 cgaaattcat ggtgagcctg agcacggcgg ggcagccggg gccgatccgg ttgcagatgc
      121 ggctgcacga caccgagtgg cgggtggatc gcgtctggct tccgccacat ctgctggacc
      181 agcaggacgc ccggacctga cggctggtgt cctaaccccg catgatctgc tccaccgcgg
      241 cggcgatgcc gaacaggcgg gcgtcgccga gggtttcgcc catcagcatc aggccggccg
      301 gagcctcccc ctcacggtgg cagggcaggc tgatcgcgca gcgatcgagg aaatttccca
      361 gcgcggtgtt gcgtaggatc agcaggttct gccggttgta gtcgcgttcg tcctccagct
      421 catccagccg cggcgggaca atggggctgg tcggcatgac cagcgcgtcg aagctctggg
      481 tttcggcatt gaattccgca atcaggcggg cacgggcagc ggtcagatcc aggtagtcgg
      541 cggcgtccat ctgcgccccg cgctcaatgc gcaggcggat acgtgggtcg tagcccgcgc
      601 cagcgcgcgc cagcagagcg cggtgccacg cataggcttc cgaagcggca aatccgccct
      661 tggcattcac ggccggcagc gcgtccaggg ccgcaaagcg gcggcgctcg atccgtgcac
      721 cggcggcaga cagcgcggcc agcacccggt cgaacgcggt ggcaacatgg gtgtccgtgc
      781 cctccagcac catgttctcg gggacggcca gccgcagccc ggccagtgct gccaacgcgg
      841 gtgcgacggg cggcgtacct gccagcaccg catcgatcac cgcgcagcac gccaccgacg
      901 gtgccagcgg gccgactgaa tccaggcttg gcgccagcgg caatacgccg tcgatcggca
      961 cgcgccgcgc cgtcggcttg tagccgacga tgccgcagaa cgccgccggg atgcggcatg
     1021 agccgccggt atcggtgccg agacccgcgg caaccatgcc gtcggccacg gccacggccg
     1081 tgccggacga gctgccgcct ggaatccgcc gccccgcccg atcccatggg ctgagcggcg
     1141 tgccgtaatg cgggttgatg ccaaggccgg agaaggcgaa ctccgtcatg ttggtccgtc
     1201 ccatcggcac gaaccccgcc gccagcatgc gctgcaccac cggtgcatgc gacgccgccg
     1261 gcggtgcgtc ggccagcacg cgggatcccg ccggggtcgg ctctcccgct acatcgaaca
     1321 gatccttcag cccgatcggg attcctgccc agcgcgtcgg ggcgcggccg gcgcggcgca
     1381 gcgtgtccat cgcatccgcc attgcccgcg ccgaggcggc atagacccgg atgaaggccc
     1441 ggccaccttc gccgcccgga tcggcgatcc gcgccaggct gtcctccacc aacgcccggc
     1501 tggtggtgtg gtcctgttcc aggccctggg cgagttgctc gattgtgcgc atgattgggt
     1561 ccccacgtga cggaacggag catgccatga ccgacaagcc agccaaaccc gtcctgctga
     1621 ccggagcctc cggcgcgctg gggcgggtgc tgacgaagtc gctggcggcc gaaggctgga
     1681 cgttgcggct gaccgacatc gcgccgttcc ccgacccgat accggccggt gccgatttca
     1741 cccgggccga tctgaacgac ggcgtcacca tgttgcgcct ggcggaaggc tgcggcgcta
     1801 tcatccacct gggcggcgtc tcggtcgaac gaccctttga agaggtgctc ggcccgaaca
     1861 tccgcgggct gtatcacatc tatgaggcgg cgcggcggga aagtgcgcgg gtgatctttg
     1921 cctcctccaa ccattccatc ggctttcatg agcgcagcga atcgctgccc gccggcgcgc
     1981 agttcctgcc ggatgggttc tatggcctgt cgaaagcgta tggcgagctg atgggccggt
     2041 tgtactggtt caagcatggg gtggagagcg tgtttatccg catcggctcc gcgtttccgg
     2101 aaccggtgga cgcccgcatg ctggcgacgt ggttgtccta tccggacctg tcgcggctga
     2161 tgatccgctg cgtggtgagc gagaaggtgg gatgttgcgt ggtgtggggc gcatccaaca
     2221 acagccggat gacctggtgg cgggacgatg cgcgcgatgc gctgggctgg gcgccgcagg
     2281 atagcgccga tcccttcgcc gggcagttgg ccggcaaggt gagcagcgac ccggtgcagg
     2341 agcgctatat gggcggcggg ttctgcgcga tcggctattc ccggtcggcg cccgccccgg
     2401 ccgagctgtt cgactgaatg cgatggatca cgccggcatg ccgcagctgc gcgatgaccg
     2461 gggatgcccg ctgttcacgc ggctaccccg ccgcgcgcgg ccgcactccc aggatatgcg
     2521 cgatcgcgta ggtcagctcc ggccggttca gcgtatagaa gtggaattca tccactccgt
     2581 tggcctgtag cagccgcacc tgctccgccg tgaccaccgc cgccaccatg cggcggatct
     2641 ccggatcgtc gtctgtccct tcgaacatat gccccagcca gtccggcacg gccgaaccgc
     2701 acagcgccgc catccgccgc agcccggcga ggttggtcac cggcatgatg cccggcacga
     2761 tcggcgcggt gatcccggcc gccaaggcgc ggtccaggaa gcgcagaaac acgccggtat
     2821 cgaagaagaa ctgggtgatc gcccgcgtcg cccccgcgtc cagcttgcgc ttcagattgt
     2881 ccagatccgc atccgggctc agggccgacg ggtgtgtctc cggataggcc gccaccgaga
     2941 tctcaaaatc cgccacgcgt cgcaagccgg ccacgaggtc ggcagcgaac gcgtagcctc
     3001 ccggatgcgg ggcatactcg ctgcccggag gcgggtcacc gcgcagggcc accacgtggc
     3061 gcaccccggc ag
//
LOCUS       DASSZH010000677         2681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1073285, whole genome shotgun sequence.
ACCESSION   DASSZH010000677 DASSZH010000000
VERSION     DASSZH010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073285"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1979
                     /locus_tag="VFN42_14615"
     CDS             <1..1979
                     /locus_tag="VFN42_14615"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET8997899.1"
                     /translation="LAPHLGATGHGNRAAVEEHSGRRVMVATQGPFALALAVVDPQQR
                     DALGAASAGYVGVSDGWHDFEQNGALTWSYGNAGPGNVALIGELPRNGALGVGFGSSA
                     KSAATLAVTSLLQPFENILRQHIAEWGEWHARRSERYAPPIDLPEGVAEQFLVSTTVL
                     RTHLDKTYPGAMVASLSIPWGDTGDERGGYHLVWPRDLVSCASALLALGAEEETRDTL
                     RYLIATQHADGHWNQNQWLGGKAFWEGLQLDETAFPVLLAAALAERSALGGIDVTDMM
                     RKALSYIACNGPVTGQERWEENGGVNAYTLAAIIAALVEGAAFLPAASQAWALDLADF
                     WNASIEQWTAVSGTDLATRIGVAGYYVRIAPPEVLAMGAAAMRELVPVRNHTGQVEVP
                     ASELIGTDFLQLVRFGIRLPDDPLMRDSITVVDNLLKVETPYGPVWRRYTGDGYGEHD
                     DGSAYDGSGRGRPWPLLAGERGHFDVSGGHDPLPALTAMCGMASPLGLIPEQIWDGVD
                     IPARELKFGRPSGSARPLAWAHAEFAKLVISRQLGHPCDRPQAVWRRYQGRRPQPSRA
                     FWTLMSPIGEIPRGTRLVVALPRPAVVRWHSEGQTGAEVATDDTGLGLHAVELNTADF
                     ASGRQIAFAWTWREEGGATEDGFRVTVGGNPTEG"
     gene            1983..2267
                     /locus_tag="VFN42_14620"
     CDS             1983..2267
                     /locus_tag="VFN42_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997900.1"
                     /translation="MKTIIEDTRFPAATRIRIGGGPDELMFVRCSFEGGEIVIEREVD
                     RTVFSQCVFRGTRFSGQSLSNRIADTCHTVLSYTEETAPPPAARAGRFRN"
     gene            complement(2441..>2681)
                     /locus_tag="VFN42_14625"
     CDS             complement(2441..>2681)
                     /locus_tag="VFN42_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997901.1"
                     /translation="GKPGADAVIGGDMVHSPLQARYPELGMRADYDSAQAGASRRTVF
                     GKLCDTSTLLCTGHFASPSKGRLTRWGDGFRYNPI"
BASE COUNT          439 a          862 c          923 g          457 t
ORIGIN      
        1 tgctggcgcc gcacctgggc gcgaccgggc acggcaaccg cgccgccgtg gaggagcatt
       61 ccggccggcg tgtcatggtc gccacccagg gcccgttcgc cctcgccctc gccgtcgtcg
      121 atccgcagca gcgtgacgca ttgggcgcgg cgagtgccgg ctatgtcggc gtcagcgacg
      181 gctggcatga tttcgaacag aacggcgcac tgacatggtc gtacggcaac gccgggccgg
      241 gcaacgtcgc cctgatcggt gaattaccaa gaaacggtgc ccttggcgtc ggtttcggca
      301 gcagtgccaa gtccgccgcg accctcgcgg tcaccagcct gctgcagccg ttcgaaaaca
      361 tcctccgtca gcacatcgcc gaatggggcg aatggcacgc ccgccgcagt gagcggtatg
      421 cgccgccgat cgatttgccc gaaggggtgg ccgagcagtt tctggtatcg acgacggtgc
      481 tgcgcacgca tctggacaag acctatcctg gtgcgatggt ggccagcctg agcattccct
      541 ggggcgacac cggcgatgaa cgcggcggct accacctggt ctggccgcgc gacctggttt
      601 cctgcgccag cgccctgctg gccctgggtg ccgaggagga aacccgtgac acgctgcgct
      661 acctgatcgc gacgcagcat gccgacggcc actggaacca gaaccaatgg ctcggcggca
      721 aggcgttctg ggaagggctg caactcgacg aaacggcctt cccggtcctg ctcgccgcgg
      781 cgctggcgga acgcagcgcg ctgggcggca ttgatgtcac cgacatgatg cggaaggcat
      841 tgagctatat cgcctgcaac ggccccgtca ccggccagga acggtgggag gagaacggcg
      901 gcgtcaatgc ctataccttg gctgcgatca tcgcggccct ggtggaaggc gccgcgttcc
      961 tgccggccgc gtcacaagcc tgggccctgg atctcgcgga tttctggaat gccagcatcg
     1021 agcagtggac cgcagtctcc gggacagatc tggcgacgcg tatcggcgta gccggctatt
     1081 acgtgcggat cgctccgccg gaggtgcttg cgatgggggc cgcggcgatg cgcgaactgg
     1141 tccccgtgcg caaccatacc ggacaagtgg aggtcccggc gtcggagctg atcggaaccg
     1201 atttccttca actcgtgcgc tttggcatcc ggctgccgga cgatcccctg atgcgggaca
     1261 gcatcaccgt cgtggacaat ctgctgaagg tggaaacacc ctatggcccg gtgtggcgtc
     1321 gctataccgg cgatggctat ggcgagcatg atgacggttc ggcgtatgac ggcagcgggc
     1381 gagggcgccc atggccgctg ctggccgggg agcgtgggca tttcgacgtc tccggcggcc
     1441 acgatccgct gccggcgctg acggcgatgt gcggcatggc aagccccctc ggcctgatcc
     1501 ccgaacagat ctgggacggc gtggacatcc cggcccgcga actgaaattc ggccgcccga
     1561 gcggttcggc tcggccgttg gcctgggcgc atgcggaatt cgccaagctg gtgatttccc
     1621 gccagcttgg gcatccctgt gatcggcccc aggcggtttg gcggcgctac caggggcgcc
     1681 ggccgcaacc aagccgcgca ttctggacgt tgatgtcacc aattggagag attccgcgcg
     1741 gcacgcgtct tgttgttgcg ttgccccggc ccgccgtcgt tcgttggcac agcgagggcc
     1801 agaccggcgc cgaagtggcg acggacgata ccgggctggg actgcacgcg gttgaactga
     1861 acactgcgga ctttgcgtcc ggtcgccaga tcgcgttcgc ctggacctgg cgggaggagg
     1921 gaggcgctac cgaggacgga ttccgcgtga cggtcggagg taatccaacg gagggctgat
     1981 cgatgaaaac gatcattgag gatacacggt ttcctgccgc gacgcggatc cgcatcggcg
     2041 gcggcccgga cgaactgatg ttcgtgcggt gctccttcga gggcggggag atcgtgatcg
     2101 agcgggaggt tgaccgcacg gtgttttccc agtgcgtttt ccgcggcacg cggttcagcg
     2161 gccaatcgtt atcgaaccgt attgcggaca cgtgccacac ggtgctgtcg tacaccgagg
     2221 agacagcacc gccgcccgcc gcacgggccg gccggttccg gaactagatc atgatcgggt
     2281 caggttgacc cgatcatgct caagaagtct ttgtttgatc gcatgattca cgtcttccgg
     2341 ctttcagcct caggcgatca tgctcttgca cgccggcaag tgccgcgacc ggcctcgcca
     2401 gggaccgcgc cgcggcgctg tatcgcggcg cggtgccggc tcagatcggg ttgtagcgga
     2461 agccgtcacc ccagcgggtc aggcgacctt tcgatggcga cgcgaaatgc cccgtgcaca
     2521 gcagcgtgga cgtatcgcac agcttgccga acacggtcct gcgggaagcg cccgcctgcg
     2581 ccgaatcata gtccgcgcgc atgccgagtt cggggtaccg ggcctgcagc ggcgaatgca
     2641 ccatgtcacc gccgatcact gcatccgcgc ccggcttgcc g
//
LOCUS       DASSZH010000678         3951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_84676, whole genome shotgun sequence.
ACCESSION   DASSZH010000678 DASSZH010000000
VERSION     DASSZH010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84676"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VFN42_14630"
     CDS             <1..888
                     /locus_tag="VFN42_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997902.1"
                     /translation="LEYRATERGFSVIAQYFGTTRVATLLARISRGILRAMALERVLL
                     ARAARGRDLVCFQPRRRPEEREDQPQDGEQQQQQQQQQKKPPQRRPAREPLPEFPSLE
                     QLVEDTRRHPVGRAIADICRDLGVSSGLCEGWFGTVLLQAMSWYRGNFTRYYKDIQKR
                     EREFAEEWDKDPTGKYDWPVRTSEGVRRVLGFFIGENALKPGPAPLLSGLALAAATGP
                     PGPHPRLFRTERTGPDHGRPRRSDSCHRLEHASGNGPHAGCPDSAAMTIVHPASGTVA
                     GPPHSGGDLCRGGQAQPAR"
     gene            895..1197
                     /locus_tag="VFN42_14635"
     CDS             895..1197
                     /locus_tag="VFN42_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997903.1"
                     /translation="MADLHKLLFETPAKVTDREVLATARLNARVKQFAGHVLDDGIID
                     RINDAIADESDRLIADGVLRERPDWLAAIVSRRLYVAFGSDAVQQLSRELRRMGAV"
     gene            complement(1298..2521)
                     /locus_tag="VFN42_14640"
     CDS             complement(1298..2521)
                     /locus_tag="VFN42_14640"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8997904.1"
                     /translation="MHSIETRSSWVVAWTALAIVSVAFGAPQIVVVALRPIAAQLGDS
                     RAIPSAAYSLAWLGMAVGGVGMGWIADRIGTRYTVAFGAAMIAIGLMLASGGKAWQLV
                     VGYGLFVGLLGNAGLNAPLYVYVSRWFDRRRGTALALISSGQYVAGALWPMLFEQAIQ
                     RLGWPHTMRVFGVFQVVVVLPAALLVLRAPPEPVQTGATADGPRPGHAVLGMPANLTL
                     LLLAVASFCCCVPMAMPQGQLVAFCGDLGIPAEQGAAMLSVLLACAFASRQFWGWLSD
                     RIGGLSTVVICSACQLVSMAGFMLARSELDLFLVSILFGLGFSGLIPAYVLAIRELFP
                     SSEAGWRVPSLLLLSGSGMATGGWLAGWIFDRTGFYGAAFAAGMGFNLLNLLVVGFLL
                     MQARQLRQAAAMRTA"
     gene            2725..>3951
                     /locus_tag="VFN42_14645"
     CDS             2725..>3951
                     /locus_tag="VFN42_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997905.1"
                     /translation="MHEVVRQGLYDPRNEHDACGVGFVVNIKGRKSHDIIHQGLKILV
                     NLDHRGAVGADPLVGDGAGCLIQMPDLLLREWATAHGKTLPAEGRYAVAMCFLPQDDR
                     SREYAIQHFEQVVKTEGQAMIGWREVPTDLAGLGQRVIDTMPVIRQAIIAASPKIRDQ
                     DAFERKILAIRKQILNNIRDIGEQKHLPGLGELYMPSFSTRTMVYKGLLLAPQVESFY
                     EDLRNPLTQSALALVHQRFSTNTFPSWKLAHPYRFLAHNGEINTVRGNVNWMYARRRS
                     MESELLGADLGKMWPLIPHGQSDTACIDNALEILVAGGYSLAHAMMMLIPEAWAGNPL
                     MDPERRAFYEYYAALMEPWDGPAAIAFTDGRQIGATLDRNGLRPARYIVTNDDHVIMA
                     SEAGVLPVPDEKILRKW"
BASE COUNT          695 a         1400 c         1301 g          555 t
ORIGIN      
        1 ctggaatacc gcgccaccga gcgcggcttc tccgtcatcg cccagtattt cggcaccacc
       61 cgcgtcgcca ccctgctcgc ccgcatctcc cgcggcatcc tgcgcgccat ggcactggaa
      121 cgcgtgctgc tggcgcgtgc cgcgcgcggc cgcgacctgg tctgcttcca gccgcgccgg
      181 cggccggagg agcgcgagga ccagccgcag gacggcgaac agcaacagca gcaacagcaa
      241 cagcagaaga agccgccgca gcggcggccc gcccgcgagc cgctgccgga attccccagc
      301 ctggaacaac tggtggagga cacccgccgc cacccggtcg gccgggcgat tgccgatatc
      361 tgccgcgacc tcggcgtgtc ctcgggcctg tgcgaaggct ggttcggcac cgtgctgttg
      421 caggcgatga gctggtatcg cggcaacttc accagatatt acaaggacat ccagaagcgc
      481 gagcgcgagt tcgcggagga gtgggacaag gatcccaccg gcaaatacga ctggccggtg
      541 cggaccagcg aaggcgtccg ccgcgtgctc ggcttcttca tcggcgaaaa tgcgctcaaa
      601 ccgggtccgg cgccgctgct gtccggcctg gccctcgccg ccgccaccgg gccccccggt
      661 ccccacccac gcctattccg cacggaacga acggggccgg atcacggccg gccccgccgt
      721 tccgattcgt gccaccggct ggaacacgct tccggcaacg gtcctcatgc cggttgtccc
      781 gactctgccg cgatgactat cgtccaccca gcatccggaa ctgttgccgg cccaccgcat
      841 tcaggcggcg acctctgccg cggagggcag gcccaacctg cacggtgacc gaccatggcg
      901 gatctgcaca agctgctgtt cgaaaccccg gcaaaggtca ccgatcgcga ggtgctcgcc
      961 accgcccggc tgaacgccag ggtgaagcaa ttcgccggtc acgtgctgga cgacggcatc
     1021 atcgaccgca tcaacgatgc catcgccgat gaatcggacc ggctgatcgc cgatggcgtg
     1081 ctgcgcgaac gccccgactg gctggcggcc atcgtctccc gccggcttta tgtcgccttc
     1141 ggcagcgatg ccgtgcagca gctcagccgt gagctgcggc gcatgggcgc cgtctgagcg
     1201 ccgcgccgcc gccggcctgc gagccggcgc cgttcgatcc gttccgctcc ctggccgcac
     1261 aaggtccgtc cgttctgctg cgctgacggt gcctgcctca cgcggtccgc atcgccgccg
     1321 cctgacgtag ctggcgggcc tgcatcagca ggaagccgac caccagcagg ttcagcaggt
     1381 tgaagcccat ccccgcggcg aaggcggcgc cgtagaaccc ggtgcggtcg aagatccagc
     1441 ccgccagcca gccgccggtc gccatgccgg aaccgctgag cagcagcagc gacggcaccc
     1501 gccaaccggc ctccgatgac gggaacagct cgcggatcgc cagcacatag gccgggatca
     1561 ggccgctgaa tcccaggccg aacaggatgg agaccaggaa caggtccagc tcactgcgcg
     1621 ccagcatgaa ccccgccatg gagaccagct ggcaggcgga gcagatcacc accgtgctca
     1681 gcccgccgat ccggtcggac agccagcccc agaattgccg gctggcgaag gcgcaggcca
     1741 gcagcacgga cagcatggcc gccccctgct ctgccggaat ccccaggtcg ccgcagaacg
     1801 ccaccagctg cccctgcggc atggccatcg gcacgcagca gcagaaggac gccaccgcga
     1861 gcagcagcag ggtcaggttc gccggcatgc ccagcaccgc gtgaccgggc cgcgggccgt
     1921 ctgccgtggc gccggtctgc accggctccg gcggggcgcg cagcaccagc agtgcggcgg
     1981 gcagcacgac caccacctgg aacaccccga acacccgcat ggtgtgcggc cagccgagcc
     2041 gctggatggc ctgctcgaac agcatcggcc acagcgcgcc ggcgacgtat tggccggagg
     2101 agatcagtgc cagggcggtg ccgcggcggc ggtcgaacca gcgggagaca tagacataca
     2161 gcggggcatt cagcccggca ttgcccaaca gcccgacgaa caggccataa ccgaccacca
     2221 actgccaggc cttgccgccg ctggccagca tcagcccgat ggcgatcatc gccgcgccga
     2281 atgcgacggt gtagcgggtg ccgatccggt cggcgatcca gcccatgccg acgccgccca
     2341 ccgccatgcc gagccaggcc agggaatagg cggcggaggg gatggcgcgg gaatcgccca
     2401 gttgtgcggc gattggccgc agcgcgacga cgacgatctg tggcgccccg aatgccaccg
     2461 acacgatcgc cagcgcggtc caggcaacca cccaggagct gcgggtttcg atgctgtgca
     2521 tgggtgattc ggtaacaggc tttcccgggc tggcaaagtg ggcgccccgg ggaccggacc
     2581 cagtcgccgt caatgcgctt gccggctggt caccgatcgc ttacgttgca ctgcggcagg
     2641 agtgttgccc ttttttgcct gtctgggcaa aagcccctcc ccggcgcccc aatttgttac
     2701 gccgttccag gaaagacccc ggctatgcat gaggttgtga gacaaggcct gtacgatccc
     2761 cgcaacgagc acgacgcttg cggcgtcggc ttcgtggtga acatcaaggg ccgaaagtcg
     2821 cacgacatca tccaccaggg cctgaagatc ctggtgaatc tcgaccatcg cggcgcggtc
     2881 ggcgccgatc cgctggtcgg cgacggtgcc ggctgcctga tccagatgcc ggacctgctg
     2941 ctgcgcgagt gggccacggc acatggcaag accttgccgg cggagggccg ttacgccgtc
     3001 gccatgtgct tcctgccgca ggacgaccgc tcccgcgagt atgcaatcca gcatttcgag
     3061 caggtggtga agaccgaagg ccaggcgatg atcggctggc gcgaggtgcc gaccgacttg
     3121 gccgggctgg gccagcgcgt aatcgacacc atgccggtga tccgccaggc gatcatcgcc
     3181 gccagtccga agatcaggga ccaggatgcg ttcgagcgga agatcctggc gatccgcaag
     3241 cagatcctga acaacatccg ggatatcggc gagcagaagc acctgcccgg gctgggcgag
     3301 ctgtacatgc cgtccttctc cacccggacg atggtgtaca agggcctgct gctcgccccg
     3361 caggtggaaa gcttctacga ggacctgcgc aatccgctga cccagtcggc gctggcgctg
     3421 gtgcatcagc gcttctccac caacaccttc ccgtcgtgga agctggcgca cccgtaccgc
     3481 ttcctggcgc ataacggcga gatcaacacc gtccgcggca acgtcaactg gatgtatgcc
     3541 cgccggcggt cgatggaatc cgagctgctg ggcgccgatt tgggcaagat gtggccgctg
     3601 atcccgcatg gccagtcgga caccgcctgc atcgacaacg cgctggagat cctggtggcc
     3661 ggcggctact cgctggcgca tgcgatgatg atgctgatcc cggaggcctg ggcgggcaat
     3721 ccgctgatgg atccggagcg gcgcgccttc tacgaatact acgccgcgct gatggagccg
     3781 tgggacggcc cggcggcgat cgccttcact gacgggcggc agatcggcgc cacgctggac
     3841 cgcaacgggc tgcgtccggc gcgctacatc gtcaccaacg acgaccacgt catcatggca
     3901 tcggaagccg gcgtgctgcc ggtgccggac gagaagatcc tgcgcaagtg g
//
LOCUS       DASSZH010000679         2078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1022149, whole genome shotgun sequence.
ACCESSION   DASSZH010000679 DASSZH010000000
VERSION     DASSZH010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022149"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..1300
                     /locus_tag="VFN42_14650"
     CDS             5..1300
                     /locus_tag="VFN42_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET8997906.1"
                     /translation="MLQQRIEAGRPVRVGLIGAGKFSSMFLAQVPTTPGIEVAAIADL
                     APDRARDACRLVGWDEDRIAATRFTDDAMAMLASADIEVVVEATGHAPAGIAHARRAI
                     AEGKHIVMVNVEADVLAGPALARDAASAGVVYSLAYGDQPALICEQVDWARSCGFRVI
                     AAGKGTKYLPAYHASTPATVWGYYGLTEEQARIGRMNPQMFNSFLDGTKSGIEMSAVA
                     NATGLTPPPDGLAFPPCGTHELAEVLRPGNAGALHHAGQVEVISSLHRDGSAVTNDLR
                     WGVYVVFEAPTEYAMRCFKEYGVVTDSSGRCTGLYRPFHLIGLELNVSILSAALRGEP
                     TGAPTGFRGDVVSTAKRDLRAGEMLDGEGGACVWGKLMPAAHSLRIGGLPIGLANRVK
                     LLRDVPAGAPLTWDDVAMDTEDPAYRYRRAMETTFAGAD"
     gene            1301..2038
                     /locus_tag="VFN42_14655"
     CDS             1301..2038
                     /locus_tag="VFN42_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6065 family protein"
                     /protein_id="HET8997907.1"
                     /translation="MDFICYRHAGWDPQIRPAESTRPWMDATPEAFAYRCLPLNIANA
                     HGWEILSPCDVQACWTGGARVEDVLLRVPPQVPADRRPVSLFGQATLTFHIQGLFRTP
                     PGWNLWVGGSPNRAKDGIAPLTGVVETDWSPYTFTMNWRFTRRNHWVRFAAGEPICFV
                     FPVQRAALEQFTPKFRALESDAELAAQFAAWSQSRNNFQSQVAKAPPPAASDKWQKRY
                     YRGLDMQGATPAADHRSKLRLKPFAED"
BASE COUNT          307 a          706 c          733 g          332 t
ORIGIN      
        1 caggctgttg cagcagcgga tcgaggccgg ccgcccggtg cgcgtcgggc tgatcggcgc
       61 cggaaagttc agctcgatgt tcctggcgca ggtgccgacc acgccgggca tcgaggtggc
      121 ggcgatcgcc gacctggcgc cggaccgcgc gcgcgacgcc tgccggctgg tcggctggga
      181 tgaagaccgc atcgccgcca cgcgctttac cgatgacgcg atggcgatgc tggcgtcggc
      241 cgacatcgag gtggtggtgg aagccaccgg ccatgcgccg gccggtatcg cccatgcccg
      301 gcgcgccatt gccgagggca agcacatcgt catggtgaac gtggaggccg acgtgctcgc
      361 cggccctgcc ttggcgcgcg acgcggcctc ggccggggtg gtgtattcgc tggcctacgg
      421 cgaccagccc gcgctgatct gcgagcaggt ggactgggcg cgatcctgcg gtttccgcgt
      481 catcgcggcg gggaagggaa ccaagtacct gccggcgtat catgcctcca cgccggccac
      541 ggtgtggggc tattacggcc tgaccgagga gcaggcgcgc atcgggcgga tgaacccgca
      601 gatgttcaac tccttcctcg acggcaccaa gtccggcatc gaaatgtccg cggtcgccaa
      661 cgccaccggg ctgacgccgc cgccggacgg gctggcgttc ccgccctgcg gcacgcacga
      721 actggcggag gtgctgcgcc ccggcaacgc gggcgcgttg catcatgccg ggcaggtgga
      781 ggtgatttcg tcgctgcacc gcgacggcag tgcggtgacg aacgacctgc gctggggcgt
      841 gtatgtggtg ttcgaagcgc ccaccgaata cgccatgcgc tgcttcaagg aatacggcgt
      901 ggtgaccgac agctccggcc gctgcaccgg gctgtatcgg ccgttccacc tgatcgggct
      961 ggagctgaac gtctccatct tgtcggctgc cctgcgcggc gagccgaccg gcgcgcccac
     1021 cggattccgc ggcgacgtgg tgtccaccgc caagcgcgac ctgcgcgcgg gagagatgct
     1081 ggatggcgag ggcggcgcct gcgtctgggg caagctgatg cccgcggcgc acagcctgcg
     1141 catcggcgga ttgccgatcg gcctggccaa ccgggtcaaa cttctgcgtg atgtgcctgc
     1201 cggcgcgccg ctgacatggg atgatgtggc gatggatacc gaagatcccg cctatcgcta
     1261 tcgccgcgcc atggagacga cattcgccgg agccgactga atggatttca tctgctatcg
     1321 ccatgccgga tgggacccgc agatccgtcc ggcggagtcc acgcgcccct ggatggacgc
     1381 cacgccggag gccttcgcct accgctgcct gccgctgaac atcgccaacg cgcatggctg
     1441 ggagatcctg tcgccctgcg acgtgcaggc ctgctggacc ggcggggcgc gggtggagga
     1501 cgtgctgctg cgggttcccc cacaggtgcc ggccgaccgg cggccggtgt cgctgttcgg
     1561 ccaggcgacg ctgaccttcc acatccaggg cctgttccgt accccgcccg gctggaattt
     1621 gtgggtcggc ggttcgccga atcgcgccaa ggacggcatc gcgccgctga ccggcgtggt
     1681 ggagaccgac tggtccccgt acaccttcac catgaactgg cgcttcaccc ggcggaacca
     1741 ctgggtgcgc ttcgccgccg gtgagccgat ctgcttcgtc ttcccggtgc agcgcgccgc
     1801 cctggagcag ttcaccccga agttccgcgc cttggagagc gacgcggagc ttgcggccca
     1861 gttcgccgcc tggagccagt cgcgcaacaa cttccagtcc caggtggcga aggcgccgcc
     1921 gccggccgcg tccgacaagt ggcagaagcg ctactatcgt ggtctggaca tgcagggtgc
     1981 cactcccgcg gcggatcacc gcagcaagtt gcgactgaaa ccatttgccg aggactagcc
     2041 gggcgaaatg cgcggcactt ccgatttatc ccatctgg
//
LOCUS       DASSZH010000680         2228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_834468, whole genome shotgun sequence.
ACCESSION   DASSZH010000680 DASSZH010000000
VERSION     DASSZH010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834468"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /gene="flgA"
                     /locus_tag="VFN42_14660"
     CDS             <1..540
                     /gene="flgA"
                     /locus_tag="VFN42_14660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HET8997908.1"
                     /translation="QLQYEAESGRFTAILSVTGAAMEPVNLRLAGRAEATEQLPVATA
                     RLPAGTVLRAEDLRLARIRVSRLHGAVVRTMQDAAGLQLRRPALPGQPLQRADLVRPS
                     LVQRGATVRLELQAGGLRLTAQGIAMQSGAAGERIRVMNTASRALLEAVVSGPDAVRV
                     LPGTAPLPRVAIGYAVMAR"
     gene            537..1319
                     /gene="flgH"
                     /locus_tag="VFN42_14665"
     CDS             537..1319
                     /gene="flgH"
                     /locus_tag="VFN42_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HET8997909.1"
                     /translation="MKSVADDTAEAGQPGRRVRPTGLATLLACLLLAGCGSLTRLSEV
                     GRPPAMTPTADPTKAPSWRPVSMPMPARQPTPNEPAALWRNGSRAFFKDQRAAQVGDI
                     VTVLVNMTDSASLKNITSASHDGAESGGMPNFFGMETLLPKTIVDPSKLLSISSGTSS
                     TGSGQIQRNEAVMLRLAGVITQVLPNGNLVVAARQEFRVNNELRELQVSGVIRPQDIA
                     SDNTVEHDRMAEARISYGGRGQLTDVQTPRWGQQVMDLLLPF"
     gene            1477..1836
                     /locus_tag="VFN42_14670"
     CDS             1477..1836
                     /locus_tag="VFN42_14670"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HET8997910.1"
                     /translation="MSISMRHLGVALALALSLGLAGCADTPDQAAPMAAPAPMAAPAP
                     APAPAMPMPAAKPLAGKDLNVAVQTALNANGAKLKVDGKMGRKTRAALRSFQHKHHLK
                     PTGHADAATLRALGIQG"
     gene            complement(1891..>2228)
                     /locus_tag="VFN42_14675"
     CDS             complement(1891..>2228)
                     /locus_tag="VFN42_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997911.1"
                     /translation="AGNYQPPGALFPALAAAQTCDGRPAAHPGIKPQPTALEGRSVLH
                     FRYYCLSSEDRIVLGDHLVAPDLDAAVRAAYEACRDHPHFPSSRIEVWQGTNRLYASG
                     HGCPAEPSL"
BASE COUNT          364 a          790 c          776 g          298 t
ORIGIN      
        1 caattgcagt atgaggccga gtccggccgc ttcaccgcga tcctgtcggt gaccggtgcg
       61 gcgatggaac cggtgaacct gcgcctcgcc ggccgcgccg aagccaccga gcagctgccg
      121 gtcgccaccg cccggctgcc ggcggggacg gtgctgcgcg cggaggatct gcgcctggca
      181 cgcatccgcg tctcgcggct gcacggtgcg gtggtgcgca ccatgcagga tgccgcgggt
      241 ctgcaactgc gccgccccgc cctgcccggc cagccgctgc agcgcgccga cctggttcgg
      301 ccatcgctgg tccagcgcgg ggccacggtg cggcttgaac ttcaggccgg cgggctgcgc
      361 ctgacggcac aggggatcgc gatgcaatcc ggagccgccg gcgagcggat ccgggtgatg
      421 aataccgcct cgcgggcgct gctcgaggcg gtggtcagcg gcccggatgc cgtgcgcgtc
      481 ctgccaggca ccgcaccgct gccgcgtgtg gcaatcggct atgcggtgat ggcgcgatga
      541 agagcgttgc agacgacacg gccgaggccg ggcagcccgg gcggcgggtt cgcccgaccg
      601 gcctggccac gttgctggcc tgcctgctgc tggcgggatg cggctcgctc acccgcctgt
      661 cggaagtcgg ccgcccgccg gcgatgacgc cgaccgccga tcccaccaag gcgccaagct
      721 ggcggccggt gagcatgccg atgccggcgc gccagccgac gccgaacgaa ccggccgcgc
      781 tgtggcgcaa cggatcgcgc gcgttcttca aggaccagcg cgcggcgcag gtcggcgata
      841 tcgtcaccgt gctggtcaac atgacggatt ccgcgagcct gaagaacatc accagtgcca
      901 gccacgacgg cgccgaatcc ggcggcatgc ccaacttctt cggcatggag acgctgctgc
      961 cgaagaccat cgtcgatccg tccaagctgc tctcgatcag cagcggcacc agcagcaccg
     1021 gcagcggaca gatccagcgc aacgaggcgg tgatgctgcg gctggccggg gtgatcaccc
     1081 aggtgctgcc gaacggcaac ctggtggtgg cggcgcggca ggaattccgg gtgaacaacg
     1141 agctgcggga gctgcaggtc agcggcgtca tccggccgca ggacattgcc tccgacaaca
     1201 cggtggaaca cgaccgcatg gcggaagcgc gcatctccta cggcggacgc ggacagctca
     1261 ccgacgtgca gaccccgcgc tggggccagc aggtgatgga tctcctgctg ccgttctgac
     1321 ccggcggacg ggaccgcgcc gcgcgtgccg gtcccggccg cacacacgcg ctggattcag
     1381 cactgcattg cgcggcgaca cattgacgag tcgcaatttt tgcggaatgc aaaggggcgc
     1441 cgcgaatcga cgcggagcga aaggacccac cccccaatgt ccatcagcat gcgccacctc
     1501 ggcgttgccc ttgccttggc gctcagtctc ggcctggccg gatgcgccga cacccctgac
     1561 caggccgccc cgatggccgc accggcgccc atggcagccc cggctccggc cccggccccc
     1621 gcgatgccga tgcctgccgc caagcccctg gccggcaagg acctgaacgt ggcggtgcaa
     1681 accgcgctga acgcgaacgg tgccaagctg aaggtggatg gcaagatggg caggaagacc
     1741 cgtgccgcgc tgcgcagctt ccagcacaag caccacctga agccgaccgg tcacgccgac
     1801 gccgccacgc tgcgggcgct cggcatccag ggctgaccgc acccacctcg cctggacaga
     1861 agggccgctg ccgcagcggc cctttcgttt tcagagggac ggctccgccg ggcagccatg
     1921 cccactggca tagagccggt tggtgccttg ccagacctcg atccggctgg acggaaaatg
     1981 cgggtgatcg cggcaggctt cataggccgc acggaccgcc gcatcgagat ccggcgcaac
     2041 cagatggtcg ccgagcacga tgcgatcctc ggaactgagg caataatagc gaaaatgcag
     2101 cacgctgcgg ccctcaagag cagtcggttg cggcttgata cccgggtgtg ccgccggccg
     2161 gccatcacag gtctgcgctg ctgccagcgc cgggaatagc gcgccgggcg gctggtaatt
     2221 tcccgcgc
//
LOCUS       DASSZH010000681         5943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_783647, whole genome shotgun sequence.
ACCESSION   DASSZH010000681 DASSZH010000000
VERSION     DASSZH010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783647"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..506)
                     /locus_tag="VFN42_14680"
     CDS             complement(213..506)
                     /locus_tag="VFN42_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET8997912.1"
                     /translation="MDRSEAISRLQAHEAELRTLGVQHLYLFGSTARGQARPNSDVDL
                     FFDHPLGAIGLYDLLDVQRAASRILGCKADVMTRRSLHRVLRQDIESSALQVF"
     gene            complement(657..1583)
                     /gene="truB"
                     /locus_tag="VFN42_14685"
     CDS             complement(657..1583)
                     /gene="truB"
                     /locus_tag="VFN42_14685"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010667833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET8997913.1"
                     /translation="MPRRKPRGRPLDGWLIIDKPPGLTSTDVVNRVRRAFDAQKAGHG
                     GTLDPLATGVLPIALGAATKTVPYVMDGTKLYRFTLRFGEARDTDDADGQVTATSEVR
                     PTDEQIRAALPPFRGSIMQVPPIFSAVKIAGERAYDMAREGRAPVLEPRPARVDRFEL
                     VERPDPDHAVFEVASGKGVYMRSLARDLAQACGTLGHIAVLRRLRVGPFTEQQAIPLD
                     KLRAAEDTAPASPDLLLPVATALADIPALALTEAEAGDLRHGQAISLVALMGRIPRTA
                     DPNGGLTRAMAGSRVIGLCRLQDGLLKPERLL"
     gene            1782..2531
                     /locus_tag="VFN42_14690"
     CDS             1782..2531
                     /locus_tag="VFN42_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET8997914.1"
                     /translation="MIELVPAPASIPPGRRVYAIGDIHGCNAQLANLHAIVAEDLDRR
                     PVDSAVLLHIGDYVDRGADTAGVLTRLARGPRIDGITVVNLLGNHDETMLHALSGDRA
                     AATDWLFAGGKAALESYGIDPDSPRDSWPQTIPAAHVDFLHGLALSHREGGYLFVHAG
                     VRPGVALDEQTREDMLRIRQPFLYTERELGAVVVHGHTPVKAPVVRHNRIAIDTGAVF
                     GGALTCLVLEGDTLGFLTAPPVGTPVSIVHR"
     gene            2724..3017
                     /locus_tag="VFN42_14695"
     CDS             2724..3017
                     /locus_tag="VFN42_14695"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HET8997915.1"
                     /translation="MPTYVMLANWTEQGMRAIDDSPKRIDVARKQLEEMGGRIQSVYM
                     TMGVHDLVITYDAPDDAVAARYTLLLGKLGNVRTSSLKAFPEEAYRQIVNSLR"
     gene            3194..3874
                     /locus_tag="VFN42_14700"
     CDS             3194..3874
                     /locus_tag="VFN42_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997916.1"
                     /translation="MPDDILSMLAEVQAQLVRNSARIAASLQRAHSAGWHLAEIRNRL
                     AAAQLVPARSGLLSDAHEQDEECSPMSETHDAGLTAHASEATRQQAELRREAAEFRRE
                     ERSHQQQTAESTRALAEEQRLISEESRLGGEEMRQAAEEARSLAELARAAAEALRAAN
                     AELWQAREEGRRLSEEIRHLAEQSRAAAEELRRDAEMERERLRQQREIFDEMLRGPGM
                     GAIPPAHQ"
     gene            complement(3994..4299)
                     /locus_tag="VFN42_14705"
     CDS             complement(3994..4299)
                     /locus_tag="VFN42_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007283678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997917.1"
                     /translation="MRRLFAAAIAAAMLAAGIGSAVAQPPPPPYARVPPPRVEHVPPR
                     PGERFVWEPGHWTWNGHRYEWIHGRYIERRHHREFVQGHWVWSHREHRWVWVGPHWR"
     gene            complement(4384..>5943)
                     /gene="ilvD"
                     /locus_tag="VFN42_14710"
     CDS             complement(4384..>5943)
                     /gene="ilvD"
                     /locus_tag="VFN42_14710"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET8997918.1"
                     /translation="CNIALNRQAQSVKTGVSEAGGTPREFTTITVTDGIAMGHQGMKS
                     SLVSRDLIADSVELTVRGHCYDALVGLAGCDKSLPGMMMAMLRLNVPSVFMYGGSILP
                     GNYRGKDVTVVDVFEAVGQHAAGKISDADLHELECVACPSAGACGGQFTANTMATVSE
                     AIGLALPGSAGSPAPYEDRDRFAVQSGRAVMQLIEQRIRPRDIVTRKALENAAVVVGA
                     TGGSTNAGLHLPAIAHEAGIRFTMDDVLAIMRRTPYIADLKPGGKYVALDVHRIGGIP
                     VIIKALLDAGLLHGDCLTVTGKTLAENHKDVVFPTDQDVVYPVGRAISQTGGVVGLKG
                     NLAPDGAIVKIAGMHNLRFEGTALCFDSEEEAFAAVQARAYKAGDVIVVRYEGPKGGP
                     GMREMLSTTSAIYGQGMGEQVALITDGRFSGATRGFCVGHVGPEAAVGGPIALLRNGD
                     RIVLDAEKGSIDMLVPEAELAARRAAWQPRRTDYNSGAIWKYAQLVGPAHLGAVTHPG
                     TEAESHCYADI"
BASE COUNT          996 a         2028 c         1988 g          931 t
ORIGIN      
        1 ggggatcagg acctcgaagc cgggtggttt accgatggcg catcgctggc ggggtgacga
       61 accgcgggat ttcgatgcca ggctcaacca gccagcgttt gcgccggtcc gcctcgaacg
      121 cttcgattga gagctctgcg agctccgcac ggatcagcgc gatcgcttcg agaatgtcgg
      181 tcagccacgc aacggggcga ggtcacgggc attcagaaca cttgcagcgc ggaggattcg
      241 atatcctggc gcagaacccg gtgcaggctg cgccgcgtca tcacatcggc cttgcagccc
      301 agaatccgcg atgcggctcg ctgcacgtcg agcaagtcgt acagaccgat cgcacccaga
      361 gggtggtcga aaaacagatc cacgtcggag ttcggacggg cctggccgcg tgccgtcgag
      421 ccgaagagat agagatgctg gacccccagc gtcctcaact cggcttcgtg cgcttgcagc
      481 ctggatatgg cttcgctccg gtccatcggg cgattttaac agaggcatga agccgcttcc
      541 agggacatgg ccggccggct gcatggcacg cttgcggacg catggcgcca cctccgccgt
      601 acgtcccgcc atctcaagcg ggatccccat aacggaggca gaactctgga acggtcctac
      661 agcaaccgct caggcttcag cagcccgtct tgcaggcggc acagcccgat cacgcggctc
      721 cccgccatgg cccgggtcaa cccgccattc gggtcggccg tccgtggaat gcggcccatc
      781 agagcgacca ggctgattgc ctggccatgc cgcaggtcgc cggcctccgc ctcagtcagg
      841 gccagcgccg ggatgtcagc cagcgcggtc gcgaccggaa gcaacaggtc cggggaagcc
      901 ggcgcggtat cctccgccgc gcgcagttta tccagaggga tcgcctgctg ttcggtaaac
      961 ggcccgaccc gcagccggcg cagcacggca atatgcccca gcgtgccgca ggcctgggca
     1021 aggtcgcgag ccaggctgcg catgtacacg cctttgccgg aagctacctc gaacaccgca
     1081 tggtccgggt cggggcgctc caccagttcg aaccgatcca cccgcgccgg acgcggttcc
     1141 agcaccggcg cccgcccctc gcgcgccatg tcataggcac gctccccggc gatcttcacg
     1201 gcggagaata tcggcggaac ctgcatgatg ctgccgcgga acgggggcag cgcggcgcgg
     1261 atctgctcgt cggtcggacg gacctcgctg gtcgcggtca cctggccgtc ggcatcgtcg
     1321 gtgtcgcgcg cctcgccgaa gcgcagggtg aagcggtaca gcttggtgcc gtccatcaca
     1381 taggggacgg tcttggtcgc ggcgccgagc gcgatcggca ggacgccggt ggccagtggg
     1441 tccagcgttc cgccatgccc ggctttctgg gcgtcgaagg cgcggcggac gcggttgacc
     1501 acgtcggtgc tggtaaggcc cgggggcttg tcgatgatca gccagccgtc cagcggacgg
     1561 ccgcgcggct tgcggcgggg catggtggtg gggtccggaa agggcgccgg gggtaagggg
     1621 gacggtccgg gctgtcaact ctccgaccgg ctggagagcc gggagggata gaccagcaag
     1681 ctggcgaagg ctgccgtgtt gcggcccccg acgaattgcc gagcatggcg gacggggccg
     1741 cacccttgcc gccccggcgc tccgctgata gccgatgcgg tatgatcgaa ctcgtgcccg
     1801 cgcccgcctc aataccgccc ggacggcgcg tttacgccat cggcgatatc cacggctgca
     1861 acgcgcagct cgccaacctg cacgcgatcg tcgccgagga cctcgaccgg cgcccggtgg
     1921 actccgccgt actgctgcat atcggcgact atgtggaccg tggcgccgac acggccggcg
     1981 tgctcacgcg gctggcacgc gggccgcgga tcgacggcat cacggtcgtc aacctgctcg
     2041 gcaatcacga cgagaccatg ctgcacgcgc tgtcgggtga ccgcgcggcg gcaaccgact
     2101 ggctgttcgc cggcggcaag gccgcgctgg agagctacgg catcgacccg gactcgcccc
     2161 gcgactcctg gccgcagacg attcccgccg cgcatgtcga tttcctgcac gggctggcct
     2221 tgtcgcaccg cgaaggcggc tacctgttcg tgcatgccgg ggtgcgcccg ggcgtggcgc
     2281 tggatgagca gacccgtgag gacatgctgc gcatccgcca gcccttcctc tataccgaac
     2341 gcgagctggg cgcggtggtc gtgcacggcc ataccccggt gaaggcaccg gtcgtgcggc
     2401 acaaccgcat cgccatcgat accggcgcgg tattcggcgg cgcgcttacc tgcctggtgc
     2461 tggaaggcga cacgctgggg ttcctcaccg caccgccggt cggtacgccg gtttccatcg
     2521 tccaccgctg atccgccggc gcgcggccgt ctccgaaggc gttccgccga atattctgac
     2581 cggcggcaat tcggcgcgaa tcatgcgttc gctccgtgcc ggctgacagt accgagcacg
     2641 cccctcgggc ggcttgccct ccggcaaaat tgggcgaaag ctggaccgct ttcgccaccc
     2701 acctgcgaac aagggagctg gccatgccga cctacgtcat gctcgccaat tggaccgaac
     2761 aggggatgcg cgcaatcgac gacagtccga agcggatcga tgtcgcgcgc aagcaactgg
     2821 aggaaatggg cgggcgcatc cagtcggtgt acatgaccat gggcgtgcac gacctggtca
     2881 tcacctacga cgcgcccgat gatgcggtcg ccgcacgcta cacgttgctg ctgggcaagc
     2941 ttggcaatgt acggacgtca tcactgaaag ccttcccgga ggaggcctac cggcagatcg
     3001 tcaactcctt gcggtagccg acccggcacc gcttgtcagg tgcgcggcgc gcggaccggc
     3061 tcgccaccac cgcattgcta tttgcgggcg cgcgcgctga gctgccggtt tcagggcagc
     3121 gaacgggtcg gcaaccgccg ggtgacatga cttggggggg cagccgcttc cagcagtgcc
     3181 gagggccgcg aacatgccgg acgacattct ctccatgctc gccgaggtgc aggctcagct
     3241 cgtccgcaac agcgcgcgca ttgcggcttc ccttcagcgg gcgcattcag cgggatggca
     3301 tttggcggaa atccggaacc gcctggccgc cgctcagctg gtgcctgccc gatcaggcct
     3361 cttgtccgac gctcacgaac aagacgagga gtgttcaccg atgtccgaga cccatgatgc
     3421 cggcctgaca gctcacgcat ccgaagccac gcggcaacag gcagagcttc gccgggaagc
     3481 ggctgagttc cgccgcgagg aaagatcgca tcaacagcag acagccgaat cgactcgcgc
     3541 cctggcggag gagcaacgcc tgatctccga ggagtcgcgg cttggcggcg aggaaatgcg
     3601 gcaggccgca gaagaagccc gctcacttgc cgaactcgct cgggcagcag ccgaggcgct
     3661 ccgcgccgcc aatgcggaat tgtggcaagc gcgtgaggag ggccgccgcc tgagcgagga
     3721 gattcgtcac cttgcagaac aaagccgtgc cgcggcagaa gagctgcgga gagacgcaga
     3781 gatggagcgt gaacgactgc ggcagcagcg cgagatattc gatgagatgc tgcgaggccc
     3841 cggcatgggt gcgattccgc cggcgcacca gtagtgggcg tgccggatcg ggtccgaacc
     3901 ggcgttcgga acccggccac agcgaacagc agccgcgatt gctactgcgg gttgacgatc
     3961 atgaacgctg ttgggtgagc gcccacggtt cggctagcgc cagtgcgggc cgacccacac
     4021 ccagcgatgc tcgcggtgcg accacaccca gtggccctgc acgaactccc gatgatgccg
     4081 ccgctctatg taacggccat ggatccattc gtagcggtgg ccgttccacg tccagtgccc
     4141 aggctcccag acgaagcgct cgcccgggcg cggcgggaca tgctccaccc gcggcggggg
     4201 caccctggca tagggcggcg gcggaggctg cgcgaccgcg ctgccaatcc ccgccgccaa
     4261 catcgccgcg gcaatcgcgg ctgcaaacaa tcgtcgcatg atcctgctcc catcaacgat
     4321 gctccggcag cgatcttacc cggaccggat gcggagcacc gtgtcgaagc gtaacgcaaa
     4381 cgctcagatg tctgcgtagc agtggctctc cgcctcggtg cccggatgcg tcaccgcacc
     4441 aagatgtgcc ggaccgacca gttgcgcgta tttccagatt gcgccggaat tgtagtcggt
     4501 gcggcgcggt tgccaggccg cgcggcgggc cgccagttcc gcctccggca ccagcatgtc
     4561 gatggaacct ttctccgcat ccagcacgat gcggtcgccg ttgcgcagca gcgcgatcgg
     4621 tccgccgacc gccgcttccg gcccgacatg gccgacgcag aacccgcgcg tcgcgccgga
     4681 gaagcggccg tcggtgatca gcgccacttg ctcgcccatg ccctggccgt agatcgcgct
     4741 ggtggtggac agcatctccc gcatgcccgg cccgcctttc ggaccttcgt agcgcaccac
     4801 gatgacgtcg ccggctttgt aggcgcgcgc ctgcaccgcg gcgaaggctt cctcctcgct
     4861 gtcgaagcac aaagcggtgc cttcgaagcg cagattgtgc atgccggcga tcttcacgat
     4921 ggcaccgtca ggcgcgagat tgcccttcag cccgaccacg ccgccagtct gcgagatcgc
     4981 cctgccgacc ggatagacca cgtcctgatc ggtggggaac accacgtcct tgtggttctc
     5041 cgccagcgtc ttgccggtga cggtcaggca atcgccgtgc agcagcccgg catccagcag
     5101 cgccttgatg atcaccggaa tgccgccgat cctgtgcaca tccagcgcga cgtatttgcc
     5161 gccgggcttc aggtcggcga tgtacggcgt gcgacgcatg atcgccagca cgtcgtccat
     5221 ggtaaagcgt atgccggctt catgcgcgat cgccggaaga tgcaggcctg cattggtgga
     5281 accgccggtg gcgcctacca ccaccgcggc attctccagc gccttgcggg tgacgatgtc
     5341 gcgcgggcgg atgcgctgtt cgatcagttg catcaccgcg cggccggatt gcacggcgaa
     5401 gcggtcgcga tcctcatagg gcgcgggcga cccggccgat ccgggtagcg ccaggccgat
     5461 cgcttccgag accgtggcca tggtgttggc ggtgaactgt ccgccgcagg caccggccga
     5521 gggacaggcg acgcattcca gctcgtgcag atcggcgtcc gagatcttgc cggcggcgtg
     5581 ctggcccacc gcctcgaaca catcgaccac ggtgacatcc ttgccgcggt aattccccgg
     5641 caggatcgag ccgccataca tgaatacgct cggcacattc agccgcagca tcgccatcat
     5701 catgccgggc agcgacttgt cgcagccggc cagccccacc agcgcgtcgt agcagtgccc
     5761 gcgcacggtc agttccacgc tgtcggcgat caggtcgcgc gacaccagcg acgatttcat
     5821 cccctggtgg cccatggcga tgccgtcggt cacggtgatg gtggtgaatt cgcgcggcgt
     5881 gccgccggcc tcagagacgc cggtcttcac cgactgcgcc tggcggttca atgcgatgtt
     5941 gca
//
LOCUS       DASSZH010000682         3532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1516768, whole genome shotgun sequence.
ACCESSION   DASSZH010000682 DASSZH010000000
VERSION     DASSZH010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516768"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VFN42_14715"
     CDS             <1..485
                     /locus_tag="VFN42_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HET8997919.1"
                     /translation="RLVVVGAVHIAQALVPFATQCGFAVTVVDPRRSFASDERFPNVT
                     VSTEWPDEAMDALEPDARTAVVTLTHDPKLDDPALDRALKSPAFYIGALGSRRTHAAR
                     LNRLRALGHTDDVLTRIQGPVGLNIEAVTAPEIALSIIAEIVGVRRGAPLPAKAKLAA
                     "
     gene            482..2137
                     /locus_tag="VFN42_14720"
     CDS             482..2137
                     /locus_tag="VFN42_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding/glycosyltransferase family
                     2 protein"
                     /protein_id="HET8997920.1"
                     /translation="MRFGPAPLDEARGAIMAHSQRVGDRMIRKGALLDDDAIAALRES
                     GRTEVICARLEPGDVPEDVAADRLATPLVSPLLARSRAATGRVNLAAEAPGLLRVDTA
                     RIDRLNSIDESLTFGTLPDYAVVAPKDLVATIKVIPFSVPGHVLTVAEALVRQGSSPI
                     ILHPFQRLRVGLVVTELPGLKDSVTEKTIAVTEERVKALTGALLPPLRTAHEEIAVGQ
                     ALESLIGQGADLLLVVGASAVVDRRDVGPMGIVRAGGEILHFGMPVDPGNLICLGRIG
                     SKPALVLPGCARSPKLNGIDFVLSRLFAGLKVGPAEMMRMGVGGLLKEDTRPLPREKA
                     PGTPRSGAAPRSAPTIAAVVLAAGRSRRMAPHNKLLVADKTGKPMIARVVDNVLSSKA
                     RPIVVVTGHQAEQVEHALGGRPVRFVHAEDYAEGLSASLKAGIAAVPQDCAAAIVCLG
                     DMPLVTGRMLDRLLESYDREEGRLIVLPTFHGKQGNPMLWDRRFFPEILQITGDSGAR
                     FLVGKHMESVAEIEMADDAVLRDFDTTESLSTLPQRLRPETIG"
     gene            2225..>3532
                     /locus_tag="VFN42_14725"
     CDS             2225..>3532
                     /locus_tag="VFN42_14725"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HET8997921.1"
                     /translation="MSFRSLYRHGFARVAACTTRTQLADPAGNAQSILRVARECHDQA
                     VALAVFPELGVSSYAIGDLLHQSALLDGVETALVSLLEGSRDMMPLVLVGAPLRHLGT
                     LFNCAVAIHRGRLLGVVPKVHLPNYREFYETRHFVSGDGTDGGEITLAGQRVPFGPDL
                     LFAAEDRPGFVVHAEICEDLWIPVPPSSEAALAGATVLANLSASNITIGKAETRRLLC
                     QSQSARCVAAYLYAAAGAGESTTDLAWDGQASIFENGITLAETERFPAADQMAVADID
                     LDLLRHERMTMGSFDTNRRRAGRPFRTVPFRLDPPDRDVGFRRRVERFPFVPADRERL
                     EQDCYEAYSIQVSGLAQRLQAAGIKRVVIGVSGGLDSTHALIVCARAFDLLGLPRKDI
                     LAFTMPGFGTSDTTKGNAWKLMRALGVTAEELDITPAAELMLRD"
BASE COUNT          515 a         1279 c         1203 g          535 t
ORIGIN      
        1 tgcgcctggt ggtggtcggc gcggtgcaca tcgcccaggc actggtgccc ttcgccaccc
       61 agtgcggctt cgccgtcacc gtggtggatc cgcgccgctc cttcgcctcc gacgagcgct
      121 ttcccaacgt taccgtctcc accgaatggc ccgatgaggc gatggacgcg ttggagccgg
      181 atgcccgcac cgccgtggtg acgctgacgc acgatcccaa gctggacgat ccggcgctgg
      241 atcgcgcgct gaaatccccg gcattctaca tcggcgccct cggctcccgc cgcacccacg
      301 cggcccggct gaaccggctg cgcgcgctgg gccataccga cgacgtcctg acacgcattc
      361 agggtcccgt cggattgaac atcgaggcgg tcaccgcccc ggaaatcgcg ctgtccatca
      421 tcgcggagat cgtcggggtg cgccgcggcg cgccgctgcc ggccaaggcc aagctcgcgg
      481 catgaggttt ggtcccgcgc cgctggacga ggcccgcggc gccattatgg cgcacagcca
      541 gcgggtcggc gacaggatga tccgcaaggg cgccctgctg gacgacgacg ccatcgccgc
      601 gttgcgcgag tccggccgca ccgaggtgat ttgcgcccgg ctggaacccg gcgacgtgcc
      661 ggaagacgtc gctgccgacc ggcttgccac cccgctggtt tcgccgttgc tggcgcgctc
      721 ccgcgccgcc accgggcggg tcaacctggc cgccgaggcg cccggtctgc tgcgtgtcga
      781 caccgcccgc atcgaccggc tgaacagcat cgacgaatcg ctgaccttcg gcacgctgcc
      841 cgattatgcc gtggtggcgc cgaaggatct ggtggcgacg atcaaggtga tcccgttctc
      901 ggtcccgggc cacgtgctga cggtggcaga ggcgctggtc cgccaaggca gttccccgat
      961 catcctgcac ccgttccagc gcctgcgcgt cggcctggtg gtgacggaac tgccggggct
     1021 gaaggacagc gtcaccgaga agaccatcgc ggtgacggaa gaacgcgtga aggcgctgac
     1081 cggcgcgctg ctgccgccgc tgcgcaccgc gcacgaggaa atcgcggtcg gccaggcact
     1141 ggaaagcctg atcggccagg gcgccgacct gctgctggtg gtcggtgcct cggcagtggt
     1201 ggaccgccgt gatgtcgggc caatgggcat cgtgcgcgcc ggtggggaaa tcctgcattt
     1261 cggcatgccg gtggaccccg gcaacctgat ctgcctgggc cgcatcggca gcaagccggc
     1321 cctggtgctg cccggttgcg cccgcagccc gaagctgaac ggcatcgact tcgtgctgtc
     1381 ccggctgttc gccggcctga aagtggggcc ggccgaaatg atgcgcatgg gcgtcggcgg
     1441 cctgctgaag gaagacacgc gccccctgcc gcgcgagaag gcccccggca cgccccgcag
     1501 cggcgccgcg ccgcgcagcg cccccaccat cgctgctgtc gtgctggccg ccggccgctc
     1561 ccgccgcatg gcgccgcaca acaagctgct ggtggcggac aagaccggca agccgatgat
     1621 cgcccgggtg gtggacaacg tgctgtcgtc caaggctcgg ccgatcgtcg tggtgaccgg
     1681 gcaccaggcg gagcaggtgg aacacgcgct gggcggccgt ccggtgcgct tcgtccatgc
     1741 ggaggattac gccgagggcc tgtccgccag cctgaaagcc ggcatcgccg cggtgccgca
     1801 ggactgcgcc gccgcgatcg tctgcctggg cgacatgccg ctggtgaccg gccgcatgct
     1861 ggaccggctg ctggaatcct atgaccgcga ggaaggccgg ctgatcgtgc tgcccacctt
     1921 ccacggcaag cagggcaacc cgatgctgtg ggaccgccgg ttcttcccgg agatcctgca
     1981 gatcaccggc gattccggcg cgcgcttcct ggtgggcaag cacatggaat cggtcgccga
     2041 aatcgaaatg gccgacgacg ccgtgctgcg cgacttcgac accacggaat cgctgagcac
     2101 cctgccccag cgcctgcgcc ccgagacgat cggctgaggc gtctcaaggc agcccgccga
     2161 cccatggcgc cgcccccgca aacgcctcat atttcccggt aacgttcaca accggtccgc
     2221 gcccatgagc ttccgctcgc tctaccgcca cggttttgcc cgcgtcgccg cgtgcaccac
     2281 ccgcacgcag ttggccgacc cggctggcaa cgcgcagtcc atcctgcgcg tcgcgcggga
     2341 gtgccatgac caggcggtgg cgctggcggt gttcccggaa ctgggcgtct ccagctacgc
     2401 aatcggcgac ctgctgcacc aatccgccct gctggacggg gtggagaccg cgctggtgtc
     2461 gttgctggag ggctcccgcg acatgatgcc gttggtgcta gtcggcgcgc cgctgcgtca
     2521 cctcggcacg ttgttcaact gcgccgtcgc catccaccgc ggccgcctgc tcggcgtcgt
     2581 gccgaaggtg cacctgccga attaccggga gttctacgaa acccgccact tcgtctccgg
     2641 cgacggcacc gatggcggcg agatcacgct ggccggtcag cgcgttccgt tcggccccga
     2701 cctgttgttc gccgccgagg accggcccgg cttcgtggtg cacgccgaga tctgcgagga
     2761 tctctggatt ccggtaccgc cgagcagcga ggccgcactg gcgggggcga ccgtgctggc
     2821 aaatctgtcg gccagcaaca tcaccatcgg caaggcggaa acccgccggc tgctgtgcca
     2881 gtcgcaatcg gcccgctgcg tcgccgccta tctgtacgcc gcggcaggcg ccggggaatc
     2941 caccaccgac ctggcctggg acggccaagc ctccatcttc gagaacggca tcacgctggc
     3001 ggaaaccgag cgcttccccg ccgccgacca gatggcggtg gccgatatcg acctggatct
     3061 gctgcgccac gagcgcatga cgatgggcag cttcgacacc aaccgccgcc gcgccggccg
     3121 gccgttccgc accgtgccgt tccgcctgga cccgcccgat cgcgacgtgg gcttccgccg
     3181 ccgggtggag cgtttcccct tcgtccccgc cgatcgtgag cggctggagc aggactgcta
     3241 cgaggcctac agcatccagg tctccggctt ggcgcagcgg ctgcaggccg ccggcatcaa
     3301 gcgcgtggtg atcggcgtgt ccggcgggct ggactccacc cacgcgctga tcgtctgcgc
     3361 ccgcgccttc gacctgctcg gcctgccgcg aaaggacatc ctggccttca ccatgccggg
     3421 cttcggcacc agcgacacca ccaagggcaa cgcctggaag ctgatgcggg cgctgggggt
     3481 gacggcggag gagctggaca tcacgcccgc ggcggagctg atgctgcgcg at
//
LOCUS       DASSZH010000683         2363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_237842, whole genome shotgun sequence.
ACCESSION   DASSZH010000683 DASSZH010000000
VERSION     DASSZH010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237842"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VFN42_14730"
     CDS             complement(<1..406)
                     /locus_tag="VFN42_14730"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8997922.1"
                     /translation="MEFGIAIPTAADSWRLVRRAEELGFTHAWFFDTQMLSADPFVAM
                     AAAAMQTTRIKLGTGVLIPSNRIAPVAANALASLNKLAPGRIAFGISTGFTGRRTMGH
                     GAVRLADMEQYILTVQALWRGETVDFTMEGKTR"
     gene            complement(432..887)
                     /locus_tag="VFN42_14735"
     CDS             complement(432..887)
                     /locus_tag="VFN42_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HET8997923.1"
                     /translation="MSAANPLLTIGYEGCTIQDVLAELKQAGAGLLIDVRAVPQSRKP
                     GFSKRQLAAGLDEQGIRYLHLQGLGTPKPGRDAARAGHPERMEVIFRDHMQSDRAQAD
                     LAQAKLVARENRACLLCFERDHRHCHRHIVAELISAETGQPIEHLYVPV"
     gene            complement(889..1815)
                     /gene="nudC"
                     /locus_tag="VFN42_14740"
     CDS             complement(889..1815)
                     /gene="nudC"
                     /locus_tag="VFN42_14740"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HET8997924.1"
                     /translation="MTLILASRPNVYSSSPLDRIAERREDADWIEQRLSDPDTLFVPV
                     WRNRNLVRGMDGGAPEAVYISGEAAHTLRMQGGPWAFLGMLEDRAVFAVDISTADDPV
                     PLLPEELGTFVDLRTVGWGVPKPEASVLAHARGLMSWRARHRFCGVCGAVCDVRSAGH
                     VMQCTACEAQHFPRTDPAVIMLVHRGGRALLGHSQRFPRANMYSTLAGFVEPGESLEE
                     AVRREVLEESGIRVGAVHYHSSQPWPFPGNIMLGFYAEGLTEEITVDPEELIDARWFS
                     RDDMRDPEAHGFALPRADSIARRLIEDWLAAE"
     gene            1936..>2363
                     /locus_tag="VFN42_14745"
     CDS             1936..>2363
                     /locus_tag="VFN42_14745"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8997925.1"
                     /translation="MQLGITMDMSGPSPKLDIERVLEAERLGFSQAWTGEAYGTDAVT
                     PAAWMLARTTRIKVGTGIMQIPARTPACTAMTAMTLQLLSGGRFLCGLGVSGPQVVEG
                     WHGVRFGKPMTRTKEYIAIIRKILERKAPLEFEGAEYVIP"
BASE COUNT          380 a          811 c          799 g          373 t
ORIGIN      
        1 ggcgggtctt gccttccatg gtgaagtcca ccgtctcgcc gcgccacagc gcctgcacgg
       61 tgagaatgta ctgttccatg tcggccaatc tgaccgcgcc gtggcccatg gtgcggcggc
      121 cggtgaagcc ggtgctgatg ccgaacgcga tgcgccccgg cgccagcttg ttcagcgacg
      181 cgagcgcatt ggcggcgacc ggtgcgatgc ggttggacgg gatcagcacg ccggtgccta
      241 gcttgatgcg cgtggtctgc atggccgccg ccgccatcgc cacgaacgga tcggcgctga
      301 gcatctgcgt gtcgaagaac cacgcatggg tgaagccaag ctcctccgcc cggcgcacca
      361 gtcgccagga atcggcggcg gtggggatgg cgatgccgaa ttccatgcgc ggctcctcgg
      421 gttcgcggcg atcagaccgg cacgtacaga tgctcgatcg gctgcccggt ttccgcgctg
      481 atcagttcgg cgacgatgtg gcggtggcaa tgccggtggt cgcgctcgaa gcacagcagg
      541 caggcgcggt tctcccgcgc caccagtttc gcctgcgcca gatcggcctg tgcacggtcg
      601 gactgcatgt gatcgcggaa gatcacttcc atccgctccg gatgaccggc gcgcgccgca
      661 tcgcgtcccg gcttcggcgt acccagccct tgcaggtgca gatagcggat cccctgctcg
      721 tccaggccgg ctgcgagctg ccgcttggag aagcccggct tgcgcgactg cggcacggcg
      781 cgcacgtcga tcagcaggcc ggcaccggcc tgcttcagct ccgccagcac gtcctggatc
      841 gtgcagcctt cataaccgat cgtcagcaat gggtttgcag cagacatctc attccgcggc
      901 cagccagtct tcgatcagcc gccgagcgat gctgtccgcc cgcggcaggg cgaagccatg
      961 cgcctccggg tcacgcatgt cgtcccggga gaaccagcgg gcgtcgatca gttcttccgg
     1021 atcgacggtg atctcctccg tcagcccctc ggcgtagaag ccaagcatga tgttgcccgg
     1081 gaaaggccag ggctggctgg agtggtagtg caccgcgccg acgcggatgc cggattcctc
     1141 cagcacttcg cggcgcaccg cttcctccag cgattcaccg ggctccacga agccggccag
     1201 ggtcgaatac atgttggcgc gcgggaagcg ctgggagtgg ccaagcagcg ctcggccgcc
     1261 gcgatgcacc agcatgatca ccgcggggtc ggtgcgcggg aaatgctgcg cctcgcaggc
     1321 ggtgcactgc atcacatggc cggcgctgcg cacatcgcac accgcgccgc agacgccgca
     1381 gaagcggtgc cgagcccgcc agctcatcag tccgcgcgca tgcgccagga ccgaggcttc
     1441 cggcttcggc acgccccagc cgacggtgcg cagatcgacg aacgtgccga gttcctccgg
     1501 cagcagcggc accgggtcgt ccgccgtgct gatatccacc gcgaagaccg cgcggtcttc
     1561 cagcatgccc aggaacgccc aggggccgcc ctgcatgcgc agcgtgtgcg ccgcctcacc
     1621 ggaaatgtac accgcttccg gcgcgccgcc gtccatcccg cgcaccagat tgcggttgcg
     1681 ccagaccggt acgaacagcg tgtcgggatc gctgaggcgc tgttcgatcc agtcggcatc
     1741 ctcccggcgt tcggcgatgc gatccagcgg actggacgaa tacacgttgg ggcggctggc
     1801 gaggatcagg gtcacagcat cacttctcgg gcgctggggt ttcggctgtg gaccgtgcca
     1861 cgccgcaccg gcggcagcaa ccttgaccgg cccccggttc ccggcgcgtc cttaccccgc
     1921 aatcaggagg cgcgcatgca actcggcatc accatggaca tgtccggtcc gtcgcccaaa
     1981 ctcgacatcg agcgggtgct ggaagcagag cggctgggat tttcccaggc ctggaccggc
     2041 gaggcgtacg gcaccgatgc ggtgacgccg gcagcgtgga tgctggcgcg caccacgcgc
     2101 atcaaggtgg gcaccggcat catgcagatt cccgcacgca cgcccgcgtg cacggcgatg
     2161 acggcgatga cgctgcaact gctgtccggc gggcgcttcc tgtgcgggct gggcgtgtcg
     2221 ggaccgcagg tggtggaagg ctggcatggc gtgcgcttcg gcaagccgat gacccgcacc
     2281 aaggaataca tcgcgatcat ccgcaagatc ctggagcgca aggcaccact ggagttcgag
     2341 ggcgccgaat acgtcatccc cta
//
LOCUS       DASSZH010000684         6286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_817605, whole genome shotgun sequence.
ACCESSION   DASSZH010000684 DASSZH010000000
VERSION     DASSZH010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817605"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VFN42_14750"
     CDS             complement(<1..273)
                     /locus_tag="VFN42_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HET8997926.1"
                     /translation="MSQLTLDQAQTIVSVALQHARAQKFQPLAVIVLDARGVLKAAGV
                     EDGTSLRRSEIATGKAHGALSFGVGSRNLAKRAEERPHFIAAVTHAV"
     gene            complement(357..1604)
                     /locus_tag="VFN42_14755"
     CDS             complement(357..1604)
                     /locus_tag="VFN42_14755"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8997927.1"
                     /translation="MASFPAPETWRAACAQDAVLASWAGPWSVCFAIASGGDTSVFAM
                     ADGCVQAESGSPAFTLRAPAAVWEKFLQPVPPRHHHGIFAMLYRVPEFAIEGDQLRFM
                     QHAHIARRVLDIGKWLSLGRTPPVPVWLNPGAGPRPAPAVTGRYVPVTVGGTTYQVYH
                     ETAGTGRDILCLHTAGADARQFHGLMADPRIIGRHRLVAFDLPWHGKSPPPDGAIPGS
                     WRLNTDLYVDLIMGFIAAAGLQRPIVLGASMSGEICLELAYRHPDAFTAIVACEASEK
                     IERRQTHWAAHPQVNQAFFVPEWIRALCAPQSPAEYCADICWQYGQGGPAVFFGDIAF
                     YSGDWDARERVGRIDTNRCRLFMLTGEYDYSCTVEHSAATAAKIPGVRFQPMQGIGHF
                     PFAENPRLFAEYLLPILQELAEG"
     gene            complement(1696..2700)
                     /locus_tag="VFN42_14760"
     CDS             complement(1696..2700)
                     /locus_tag="VFN42_14760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8997928.1"
                     /translation="MDQRPPRLAILLLPKLDHFARELVAGLPVATGWEVRGFRLASVA
                     DFRAALDWTDRPERDAVWFEFCWPPFPRLIAATAFAGRRVIVRVHRIEAMETDHVAHT
                     PWHKVSDVVVVSRDMAARVRQAAPGLDATTRLHLIHNGLDLERFVPLQAWNPYRIGWC
                     GLMTLRKNPTLALHILAELRRLDPRYTLHVCGQGGERLARESFAHLVRRLNLADAVRN
                     DGTLPQSAMPDWHARNGVLLHTSLHESFGYAIAEAAAAGCDLAVLDHPGADEFWPEEM
                     RFGTVAEAVAMVRAAAPHRWREHVLRRFALRRQIEATAMLLSGDPPRQQRRGVLAQFA
                     "
     gene            complement(2782..3675)
                     /locus_tag="VFN42_14765"
     CDS             complement(2782..3675)
                     /locus_tag="VFN42_14765"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8997929.1"
                     /translation="MSDTAIALRPIGTIGFIGLGVMGEPMCGHLARRFGKPVLAHDLR
                     AEPLQRLGAFGVSPAMPAQMAQRADLILLSLPDGTAVRSVIATLEPHLQPGQCLVDTS
                     TSPVALTRAIGARMAERGIGYADAPVARTREAAAKGELSIMVGASDAVFAHVQPVLRT
                     MGSDVTHCGPVGCGQVVKILNNMLVFQNTAALAEAIAIGRRNGVPPDVLLPAVALGSG
                     DSFVLRSHGMKSMLPRVFPERAFSTLYAMKDLSYALEMADASGLDVPGARLAMQRLQE
                     AARQGHGDEYHPAVLQVIDPL"
     gene            3804..4127
                     /locus_tag="VFN42_14770"
     CDS             3804..4127
                     /locus_tag="VFN42_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET8997930.1"
                     /translation="MAINNCKDRKTERFLAGERVKEFQSFADSAAKALTRLQAAVRLA
                     DLRSPPSNRFEALGGERKGQYSIRINRQYRVCFSWVPHGSTSSEPDKLQRPGDAWDVE
                     ITDYH"
     gene            4139..4444
                     /locus_tag="VFN42_14775"
     CDS             4139..4444
                     /locus_tag="VFN42_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HET8997931.1"
                     /translation="MRQPIDRLPPIHPGIFLKDELEALGLSARKFAEHIHVPHNAVTG
                     IMNGDRAISAQMAIRLGRAFGTTAQYWMNLQTIYDLKQAEAAMPAEARHIQAFQAAE"
     gene            4472..5353
                     /locus_tag="VFN42_14780"
     CDS             4472..5353
                     /locus_tag="VFN42_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET8997932.1"
                     /translation="MFADLRGTKIYFDIEGAGLVPDGDHMRERPPAMIIHGGPGGDHS
                     GFKPTFSALTDRMQLIYFDHRGQGRSGQADPAADPDRFTLDENVEDMEALRRHLGLGP
                     IVSIGTSYGGMVAMAHAARYPDAVSHLVLIVTAAHGGFVQRAEAIVRERGTPEQQRVC
                     ATLWAGGFRTREELKHYYAVMGPLYSLRHDPAGAASGRARAIHTPEPLNRAFGPDGFL
                     RRFDLRPELARITAPALILAGRHDWICPVEFSEEIHRLIPGSDLRIFANSSHSIRADE
                     PEAMLDAIRGFLVYRRG"
     gene            complement(5375..6028)
                     /gene="tmk"
                     /locus_tag="VFN42_14785"
     CDS             complement(5375..6028)
                     /gene="tmk"
                     /locus_tag="VFN42_14785"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET8997933.1"
                     /translation="MRGCFITLEGGEGAGKTTQARLLAEALAARGLSVLRTREPGGAP
                     GAEQLRDLLLRGTIDWSAPAEAMLHFAARAEHVEKTIRPALAAGRWVVCDRFADSTLA
                     YQGYGQGADRAMIAELTRLIGLEPDLTLVLDVSAEVATARLLQRGAQADRYESLDVLF
                     HARVNQGFRDIAAAAPQRCVVIQADAAAETVHAAIMHAVESRLLGNASTTAPARPPR"
     gene            6121..6270
                     /locus_tag="VFN42_14790"
     CDS             6121..6270
                     /locus_tag="VFN42_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997934.1"
                     /translation="MDDIRTEHDSSLTERKPEPAADNTVPPERRKVGPESEVRGGPEA
                     PEERE"
BASE COUNT         1088 a         2174 c         2125 g          899 t
ORIGIN      
        1 gaccgcatgg gtgacggcgg cgatgaagtg cgggcgctct tccgcgcgct tggccaggtt
       61 gcgcgagccg acgccgaagg acaacgcgcc atgtgccttg ccggtggcga tctccgagcg
      121 acgcaggctg gtgccatcct ccacgccggc ggctttcagc acgccgcggg catccagcac
      181 gatgacggcc agcggctgga acttctgcgc ccgcgcgtgt tgcagggcga cgctgacgat
      241 ggtctgcgcc tggtcgagcg tgagttggga catggtcggg ttcctcctga gtttgggcga
      301 caatggtgca tcggaaacga tctgaccatc ccggttgggc tgccggccga gccagttcag
      361 ccctcggcca gttcctgcag gatcggcagc aggtactcgg caaacaaccg cggattctcg
      421 gcaaacggga aatgcccgat cccctgcatc ggctggaacc gcacgccagg aatcttcgcc
      481 gcggtagcgg cagagtgttc caccgtgcag gagtagtcgt attcgccggt cagcatgaac
      541 agccggcagc ggttggtgtc gatgcgtccg acgcgctcgc gcgcatccca atcgccggaa
      601 tagaaggcga tatcgccgaa gaacaccgcc ggtccgccct ggccgtactg ccagcagata
      661 tcggcacagt attccgcggg gctctgcggc gcgcacagcg cgcggatcca ctccggcacg
      721 aagaacgcct ggttcacctg cggatgcgcc gcccaatgcg tctgccggcg ctcgatcttc
      781 tcgctggcct cgcaggcgac gatggcggtg aaggcatcgg gatggcggta ggcgagttcc
      841 aggcagatct cgccggacat cgaggcgccg agcacgatcg gacgttgcag gccggcggcg
      901 gcgatgaagc ccatgatcag atcgacatac aggtcggtgt tcagccgcca ggaacccggg
      961 atggcgccat ccggcggcgg cgacttgccg tgccaaggca ggtcgaaggc caccagccgg
     1021 tgccgcccga tgatgcgcgg atcggccatc aggccatgga actgccgcgc atcggcgcca
     1081 gcggtgtgca ggcacaggat atcgcggccg gtgccggcgg tctcatgata gacctgatac
     1141 gtcgtgccgc cgacggtcac cggcacgtag cggccggtca ctgccggggc cgggcgcgga
     1201 ccggcgccgg ggttcagcca caccggaacc ggcggcgtgc ggcccaggct cagccacttg
     1261 ccgatatcca gcacgcggcg ggcgatatgg gcgtgctgca tgaagcgaag ctggtccccc
     1321 tcgatggcga attccggcac gcgatacagc atggcgaaga tgccgtgatg gtgccgcggc
     1381 ggcaccggct gcaggaactt ctcccacacc gcggccgggg cacgcagggt gaaggccggc
     1441 gaaccggatt ccgcctggac gcagccatcc gccatggcga acacgctggt gtcgccgccg
     1501 gaggcgatgg cgaagcacac cgaccagggt cccgcccaac tggccagcac cgcgtcctgc
     1561 gcgcaggctg cgcgccacgt ctcgggggca ggaaaactcg ccataccagg caccggctgt
     1621 ggatgttcgg gagtcgcagg ataaccggtt ccgccaagca tgccagaccg ccggccaggg
     1681 ccggccacgg ccggttcagg caaactgcgc cagcacgccg cgacgctgct gccgtggcgg
     1741 atcgccggac aacagcatgg cggtggcctc gatctgccgc cgcagcgcga aacgccgcag
     1801 gacatgctcg cgccagcgat gcggtgcggc ggcgcggacc atcgccaccg cttcggcaac
     1861 cgtaccgaag cgcatctcct ccggccagaa ttcgtcggcg cccgggtgat ccagcacggc
     1921 cagatcgcaa cccgccgccg ccgcttcggc aatcgcgtag ccaaagcttt cgtgcaggct
     1981 ggtgtgcagc agcacgccgt tgcgcgcgtg ccagtctggc atggcggatt gcggcagcgt
     2041 gccgtcgttc cgcaccgcat cggcgaggtt gagccggcgc accagatgcg cgaacgattc
     2101 gcgggccagc cgctcgccgc cctggccgca cacatgcagg gtgtagcgcg ggtccagccg
     2161 gcgcagttcg gcgaggatgt gcagcgccag cgtcgggttc ttgcgcagcg tcatcaggcc
     2221 acaccagccg atgcgatacg ggttccacgc ttgcagcggg acgaagcgct ccaggtccag
     2281 gccgttatgg atcagatgca gccgcgtcgt ggcgtccagc ccgggcgccg cctggcgcac
     2341 ccgcgccgcc atgtcgcggc tgaccaccac cacatcgctg accttgtgcc acggcgtgtg
     2401 ggcgacgtga tcggtttcca tcgcctcgat gcgatgcacg cgcacgatca cgcgccggcc
     2461 ggcgaacgcc gtcgccgcga tcagcctggg aaacggtggc cagcagaact cgaaccagac
     2521 ggcgtcacgc tccggccggt cggtccagtc cagggcggcc cggaaatccg ccacactggc
     2581 caggcggaaa ccacgcacct cccagccggt tgccaccgga agtccggcga ccagttcccg
     2641 ggcgaagtgg tccagcttgg gcaacagcag gatggcgagc cgcggcgggc gctgatccat
     2701 ggcggtgttc cgggcgaagg catcgcccag catgcggcac aggaaccaac aaagcgttaa
     2761 ggcctccggg gctcgccgcc gctacagcgg atcgatcacc tgcagcaccg ccggatggta
     2821 ttcgtcgcca tgcccctggc gcgccgcttc ctgcagacgc tgcatggcca gccgcgcgcc
     2881 gggaacatcc agcccggacg catcggccat ctccagcgcg taggacaggt ccttcattgc
     2941 atacagggtg gaaaaggcgc gttccggaaa cacccgcggc agcatcgact tcatgccgtg
     3001 gctgcgcagc acgaagctgt cgccggagcc caacgccact gccggcagca gcacgtcggg
     3061 cggcacgccg ttgcggcggc cgatggcgat cgcctcagcc agcgcggcgg tgttctggaa
     3121 caccagcatg ttgttgagga tcttcaccac ctggccgcag ccgaccgggc cgcaatgcgt
     3181 cacgtcgctg cccatggtgc gcagcaccgg ctgcacatgg gcgaacacgg cgtccgatgc
     3241 gccgaccatg atgctgagtt cgcccttcgc cgccgcttcc cgcgtacgcg ccaccggcgc
     3301 atcggcatag ccgatgccgc gctccgccat gcgcgcgccg atcgcacggg tcagcgccac
     3361 cggggaggtg ctggtatcga ccaggcattg cccgggctgc agatgcggct ccaacgtggc
     3421 gatgaccgag cgcaccgccg tgccgtccgg cagcgacagc aggatcaagt cggcgcgctg
     3481 cgccatctgc gccggcatgg ccgggctgac gccgaaggca cccagccgct gcaagggttc
     3541 ggcgcgcaga tcgtgcgcca gcaccggctt gccgaagcgg cgggccaggt ggccgcacat
     3601 gggctcaccc atcacgccga ggccgatgaa gccgatggta ccgattggcc gcagcgcgat
     3661 ggcagtgtcc gacatggcgt atcctcctga tgcggaactg ttggtccggc ggcaggaggc
     3721 tgtcaaagca ggagcgaagc ctggcaggca gattgaccgg tatgcggcac gtaccatata
     3781 cagcagcgcc gggctccgca tcgatggcga tcaacaattg caaggaccga aaaacggagc
     3841 gctttcttgc cggtgagcgc gtgaaggaat tccagtcctt tgccgattcg gccgccaagg
     3901 cgctgacgag actgcaagcg gccgtcaggc tcgccgacct gcgttcgcca ccatccaacc
     3961 ggtttgaggc gcttggtggt gaacggaagg gccaatacag catacgcatc aatcggcagt
     4021 accgcgtatg cttcagctgg gtgccgcatg ggtcaacatc ttctgaaccg gacaagctgc
     4081 agcgtcccgg cgatgcctgg gatgtcgaga tcacggatta tcattgagga gaaccgcgat
     4141 gcgtcagccg attgaccggc ttccgccgat ccacccgggc atcttcctga aggatgaatt
     4201 ggaggccttg ggactgagcg cccgcaaatt cgcggagcat atccacgtgc cacacaacgc
     4261 cgtcaccggc atcatgaacg gagatcgcgc catttccgcg cagatggcga tccgactcgg
     4321 ccgggccttc ggcaccacgg cgcaatactg gatgaacctg caaaccatat acgacctgaa
     4381 gcaggccgag gcggcgatgc ccgccgaagc acgccacatc caagcttttc aggctgcgga
     4441 atgacccacc gtcgcgaaac aggagaccac catgttcgct gacctgcgcg gcacaaaaat
     4501 ctacttcgac atcgagggtg ccgggctggt gccggacggc gaccacatgc gcgaacgccc
     4561 gccggccatg atcatccacg gcggcccggg cggcgaccac agcgggttca agccgacatt
     4621 cagcgccctg accgaccgca tgcagctgat ctatttcgac catcgcggcc agggacgctc
     4681 cggccaggcc gacccggccg ccgatccgga tcgcttcacg ctggacgaaa atgtggaaga
     4741 catggaggcg ctgcgccgcc acctgggcct gggcccgatc gtgtccatcg gcacatccta
     4801 tggcggcatg gtggcgatgg cgcacgcggc acgctacccg gatgcggtgt cgcatctggt
     4861 gctgatcgtc accgccgcgc atggcggctt cgtccaacgc gccgaagcga tcgtgcgcga
     4921 gcgcggcacg ccggaacagc agcgcgtctg cgccacgctc tgggccggcg ggttccgcac
     4981 gcgggaggag ctgaagcact actacgcggt gatgggcccg ctgtattcgc tgcggcacga
     5041 cccggcgggc gcggcgtcag gacgcgcccg ggcgatccac acgccggaac cgttgaaccg
     5101 cgccttcggt ccggacggct tcctgcgccg cttcgacctg cgtccggaac tggcgcgaat
     5161 caccgcgccg gcgctgatcc tggctgggcg gcacgactgg atctgcccgg tggagttctc
     5221 cgaggaaatc caccgtctga tccccggttc cgatctgcgc atcttcgcca acagcagcca
     5281 ctcgatccgc gccgatgagc cggaggcgat gctggatgcg atccgcgggt tcctggtgta
     5341 ccgccgcggc tgaccgcacc ggctgcgttg cggttcaacg cggcggccga gccggcgccg
     5401 tggtcgaagc atttcccagc agccgggact ccaccgcatg catgatcgcg gcatgcacgg
     5461 tctcggccgc ggcgtcggcc tggatcacca cacagcgctg cggcgcggcg gcggcgatgt
     5521 cacgaaaccc ctggttcacc cgggcatgaa acagcacgtc cagggattcg tagcgatcgg
     5581 cctgcgcgcc gcgctgcagc agccgagcgg tcgccacttc ggcggacacg tccagcacca
     5641 gggtcaggtc cggctccagg ccgatcagcc gggtgagttc ggcgatcatc gcccgatccg
     5701 cgccctggcc gtagccctga taggccaggg tggaatcggc aaagcggtcg cacaccaccc
     5761 atctgcccgc cgccaaggcc ggccggatcg tcttctccac atgctcggca cgcgccgcga
     5821 aatgcagcat ggcctcggcc ggcgccgacc agtcgatcgt accgcgcagc aacagatcgc
     5881 gcagttgttc ggcgcccggc gcgccgcccg gctcacgcgt gcgcagcacc gacaggccgc
     5941 gcgccgccag cgcctcggcg agcagccggg cctgggtggt cttccccgcc ccctcgccgc
     6001 cttccagggt gatgaaacag ccgcgcattc cgtccctcgc cgcaccgtgg cgcagcggta
     6061 gcacaggatt cggccggcag gcatgcgcaa cccggcacga tgccgcgggt tactccggcc
     6121 atggacgata tccgcacgga gcacgacagc agcctgacgg aacgcaagcc ggaaccagcg
     6181 gcggacaaca ccgttccgcc ggagcggcgc aaggtcggtc cggaatccga ggtgcgcggc
     6241 gggccggagg caccggaaga gcgggagtaa cggtcccagg ccctcg
//
LOCUS       DASSZH010000685         3298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_425365, whole genome shotgun sequence.
ACCESSION   DASSZH010000685 DASSZH010000000
VERSION     DASSZH010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425365"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VFN42_14795"
     CDS             <1..359
                     /locus_tag="VFN42_14795"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET8997935.1"
                     /translation="LHVRFPHVGQGESTAVVHASRIRLAEQIDTASARQVAQALEVWR
                     DGMSGLDAAGCVADRLEALYARAGMPTRLRQLDIPRDALPDIARDTVKNFNANAGLRT
                     PQAQIDDALRLLQAAY"
     gene            432..2468
                     /gene="tkt"
                     /locus_tag="VFN42_14800"
     CDS             432..2468
                     /gene="tkt"
                     /locus_tag="VFN42_14800"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET8997936.1"
                     /translation="MAAIAELPADSPALARQAEILARPMTPERRLADCIRALAIDATE
                     ASNSGHPGLPMGMADVAVSLWSRFHKFDAADPRWPDRDRFVLSAGHGSMLLYALIHLT
                     GHAGMTIDDLRNFRQLHSPAAGHPEYGEHPAIETTTGPLGQGIATAVGLALGERMMAA
                     RFGKSLVDHRTWVIASDGDMMEGVSHEAGAIAGHLCLNKLAVLYDDNHVSIDGDTALS
                     FSDDVLKRFAAYGWATKRVDGHDPQQIAAALSFAMRSKKPTLIACSTIIGLGAPHKAG
                     TAAVHGAPLGAQEAAAAKQALGWHEPPFAIPDELQQRWLAIGGRGASARRSWLKRLAR
                     HSMRAEFERVIAGRLPENFHEAVAALKQGFAEGKPKLATRQSSQKCLEALVPAIPELI
                     GGSADLTGSNLTFVKNMGNISAGNYAGRYIHWGVREHGMCAASNGLALHGGIIPYTGT
                     FFVFSDYMRPSIRMGAIMRQRVIHVLTHDSIGLGEDGPTHQPVEHLASLRAMPNLHVY
                     RPADAMETAECWELAIRRTDGPSLLVLTRQALPALRTDTAENRCARGGYVLAEAEGAR
                     QATLIATGSEVSLAMAARDRLAADGIAVAVVSLPCWRLFEQQDAAYRAEVLGGAPRIG
                     IEAAAEFGWDKWVGPDGVFMGVSGFGASANYTDLYKHFGLTPEAIVATVRKRLG"
     gene            2672..>3298
                     /locus_tag="VFN42_14805"
     CDS             2672..>3298
                     /locus_tag="VFN42_14805"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET8997937.1"
                     /translation="MAVKVAINGFGRIGRLVLRAIVESGRADIVPIAINDLGSVEANA
                     HLFAYDSVHGRFPGQLEVSGDAITIRHNGKVYGPIKVSAERDPTKAPYSGVDVAMECT
                     GIFTSKDKASALLKAGARKVVISAPGDGVDATIVYGVNHKTLTKDMTIISNASCTTNC
                     LAPMAKVLHERFGILRGYMVTIHAYTGDQNTVDTLHKDLHRARAAAVSA"
BASE COUNT          541 a         1166 c         1101 g          490 t
ORIGIN      
        1 cgctgcacgt ccgctttccg catgtcgggc agggcgagtc caccgccgtg gtgcacgcgt
       61 cgcggatccg gctggccgaa cagatcgaca ccgcatcggc gcgccaggtg gcccaggcgc
      121 tggaggtctg gcgcgatggc atgagcgggc tggatgcggc gggttgcgtc gcggatcgcc
      181 tggaagcgct gtatgcccgt gccggcatgc cgacgcgcct gcgtcagctc gatattccgc
      241 gtgacgcgct gccggacatc gcgcgtgaca ccgtgaagaa cttcaacgcc aatgccgggc
      301 tacgcacgcc gcaggcgcag atcgacgacg cgctgcgctt gctgcaagcc gcctactgat
      361 cggtgcgccg gtgccaccct tgcccctccc ctgccctgcc cgtatagccg cgcggcaccg
      421 aggagaaggc catggctgcc attgcagaac tccctgccga tagccccgcc ctggcgcggc
      481 aggcggagat cctggcgcgc ccgatgacgc ccgaacggcg cctggcggac tgcatccgcg
      541 ccctggcgat cgatgccacc gaagcctcca attccggtca ccccggcctg ccgatgggca
      601 tggcggatgt tgccgtttcg ttatggtcac gcttccacaa attcgatgcc gccgatccgc
      661 gctggccgga ccgcgaccgc ttcgtgctgt ccgccggcca cggctccatg ctgctgtacg
      721 ccctgatcca tctgaccggc cacgccggca tgaccatcga cgacctgcgc aatttccgcc
      781 agctccactc ccccgccgcg ggccatccgg aatacggcga acaccccgcg atcgagacca
      841 ccaccgggcc gctcggccag ggcatcgcca cagccgtcgg cctggcgctg ggcgaacgga
      901 tgatggccgc acgcttcggc aagtcgctgg tcgatcaccg cacctgggtc atcgcctccg
      961 acggcgacat gatggaaggc gtcagccatg aggccggggc gatcgccggc catctctgcc
     1021 tgaacaagct ggcggtgctg tacgacgaca accacgtgtc gatcgacggc gacaccgcgc
     1081 tgtccttctc ggatgatgtg ctgaagcgct tcgccgccta tggctgggcg accaagcggg
     1141 tggacggcca cgatccgcag cagatcgccg cggcactgtc cttcgcgatg cggtccaaga
     1201 aaccgacgct gatcgcctgc agcacgatca tcggcctggg cgcgccgcac aaggccggca
     1261 ccgccgccgt gcacggcgca ccgctcggtg cgcaggaagc cgccgcggcg aagcaggcgc
     1321 tgggctggca cgaaccgccc ttcgccattc cggacgagct gcagcagcgc tggctggcca
     1381 tcggcggacg cggcgccagc gctcggcgaa gctggttgaa gcggctggcc cgccacagca
     1441 tgcgcgccga gttcgaacgg gtgatcgccg gacgcctgcc ggagaatttc cacgaggccg
     1501 tcgccgcgct gaaacagggc tttgccgagg gcaagcccaa gctggccacc cgccagtcca
     1561 gccagaaatg cctggaggcc ctggtcccgg cgattccgga actgatcggc ggttccgccg
     1621 acctgaccgg atccaatctc accttcgtga agaacatggg gaacatctcg gcgggcaact
     1681 atgccgggcg ctacatccat tggggcgtgc gcgaacacgg catgtgcgcc gccagcaacg
     1741 gcctggcgct gcatggcggg atcatcccgt acaccggcac gttcttcgtg ttcagcgact
     1801 acatgcgccc atcgatccgc atgggcgcga tcatgcgcca gcgggtgatc cacgtgctga
     1861 cgcatgattc gatcggcctg ggcgaggacg ggccgaccca ccagccggtg gagcatctcg
     1921 ccagcctgcg cgccatgccc aacctgcacg tctatcgccc cgccgacgcg atggagaccg
     1981 ccgagtgctg ggaactggcg atccgccgca ccgatggacc gagcctgctg gtgctgaccc
     2041 gacaggcgct gccggcgctg cgcacggata ccgcggagaa ccgctgcgcg cgcggcggct
     2101 acgtgctggc cgaggccgag ggcgcccgcc aggcgacact gatcgccacc ggctcggagg
     2161 tgtccctcgc catggcggca cgcgaccggc tggcggcgga cgggatcgcc gtggcggtgg
     2221 tctcgctgcc ctgctggcgg ctgttcgagc agcaggacgc cgcctatcgc gccgaggtgc
     2281 tgggcggcgc cccacgcatc ggcatcgagg ccgcggccga gttcggctgg gacaagtggg
     2341 tggggccgga cggcgtgttc atgggcgtgt ccggcttcgg ggcctcggcc aactacaccg
     2401 acctgtacaa gcatttcggc ctgacgccgg aggccatcgt ggccaccgtc cgcaagcggt
     2461 tggggtaggg cccccgcacc ggtcgccgga atgtgaccgc ccccgcgcgg cggcgcgcga
     2521 accgcgcttc tcacatccgg cgtggacgcc gcacgccgga acgcccggtt catgcccaac
     2581 cggatcggcc ccgcagggtt gatccgtgtc aacgtttggt taatgagttc ggtgcaggaa
     2641 gcgtcggaac ctgacaggaa agggtatccc catggccgtt aaggtcgcca tcaacggatt
     2701 tggacgcatc ggccgcctcg tgctgcgcgc catcgtcgag agcggacggg ccgatatcgt
     2761 gccgatcgcc atcaacgatc tgggcagcgt cgaagccaac gcgcatctgt ttgcctatga
     2821 cagcgtgcac ggccgcttcc ccggccagct ggaagtctcc ggcgacgcca tcaccatccg
     2881 ccacaacggc aaggtgtacg gcccgatcaa ggtatcggcc gaacgcgacc cgaccaaggc
     2941 gccctattcc ggcgtcgatg tggcgatgga gtgcaccggc atcttcacca gcaaggacaa
     3001 ggcttcggcg ctgctgaaag caggcgcgcg caaggtggtg atctccgccc cgggcgacgg
     3061 ggtggatgcg acgatcgtct atggtgtcaa tcacaagacg ctgaccaagg acatgacgat
     3121 catctccaac gcgtcctgca ccaccaactg cctggcgccg atggccaagg tgctgcacga
     3181 gcgcttcggc atcctgcgcg gctacatggt gaccatccac gcctataccg gcgaccagaa
     3241 cacggtggac acgctgcaca aggacctgca ccgcgcccgc gcggcggcgg tctcggcg
//
LOCUS       DASSZH010000686         3711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_715864, whole genome shotgun sequence.
ACCESSION   DASSZH010000686 DASSZH010000000
VERSION     DASSZH010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715864"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="VFN42_14810"
     CDS             complement(<1..1180)
                     /locus_tag="VFN42_14810"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET8997938.1"
                     /translation="MAQLELQQLQRLSIEQLSQLQVTSVEKRRQPLADAPAAIYVITR
                     EEIVDSGATTIPEMLRLAPNLNVAQVNSSGYEVSARGFNAGNNAILSNKLLVLIDGRS
                     VYTPLFGGVYWDMIGVQPQDIERIEVISGPGATLWGANAVNGVINIITRNSSDTQGGL
                     LTVGGGNYELYDAIEYGGRLSPTLTYRVHGEFTHFNAFPAPGGGNAGDAWYKPQGGFR
                     LDWNPPGQSLSLLGDFFDATEQPGGTVRGGDAMVSYNRELSGGSHLQLLAYYDNAQRW
                     SNNGLGFAVNTYDMEIQHSFKFAGWNSIVWGAGERVVDYQIHFASTSSLRYEPPRATL
                     NLANVFAQDTIALAPSLKLTLGLKLEDEPYAGVQPMPSIRLGWSPADHVFFWAAVSRA
                     V"
     gene            1597..2658
                     /locus_tag="VFN42_14815"
     CDS             1597..2658
                     /locus_tag="VFN42_14815"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8997939.1"
                     /translation="MPVAAGLLAGALAGLAFVGCGMTSAAPPPAQTRRREVVINGKRI
                     KTVDVHAHCIVPAAAALISHPLEAPGLLMHDTSTRIAAMDAQGIDVEALSINPYWYRA
                     ERDAAAELIRIQNETLVEFCAANPERFVAFATASLQYPDLAAQQVEHAVKQLGFRGVG
                     VAGSVAGEELANPKFHPFWAKCEELGVLVFLHPLGTRELEPSGRLGGSGLLTNTIGNP
                     LETTIALSHLIFEGTLDRFPGLKICAAHGGGFLPSYANRSDAVTRCFPDRVGPLPKKP
                     PTAYLRDGQLFFDTIVFTPEALRHLIAETGSSQIMVGTDYPFPWTSTEVDLVLNTPGL
                     TDDERVAILGGTASRLLGI"
     gene            2753..3211
                     /locus_tag="VFN42_14820"
     CDS             2753..3211
                     /locus_tag="VFN42_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997940.1"
                     /translation="MIRAATKLLLLFGLYVTLSGQGSPDELTAAGLCALAAAALAVTI
                     PRIAERHFAFRGVPWLRLGAGTAASLAGDLVRVGLRLARPAIPPGSFHRWPFAAGGEG
                     RRDSARRGLVTVVASLAPNTYVVAVLIGRRELLVHRLAPAPPPRDRDWPQ"
     gene            3208..3459
                     /locus_tag="VFN42_14825"
     CDS             3208..3459
                     /locus_tag="VFN42_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HET8997941.1"
                     /translation="MNPWLAAVVALLPALAAPLWIALRGSLADRLVAVQLAGTMTTVI
                     MTLMSFAFDQPSWIDLSLTLAFLALPGTLVLAIFVERWM"
     gene            3456..>3711
                     /locus_tag="VFN42_14830"
     CDS             3456..>3711
                     /locus_tag="VFN42_14830"
                     /inference="COORDINATES: protein motif:HMM:NF015302.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HET8997942.1"
                     /translation="MIAVSLLLAVLVLLLWLAAWGFARLRTPYDRIHCTSFATLAAAV
                     PLVLVAFVSDGASDRACKILLLAALMLVSGATLAHATGRAI"
BASE COUNT          556 a         1279 c         1226 g          650 t
ORIGIN      
        1 gtaccgcgcg tgataccgcc gcccagaaaa acacgtggtc ggccgggctc cagcccaggc
       61 ggatactggg catcggttgc acgccggcat agggctcgtc ttccagtttc agccccagcg
      121 tcagcttcag gctcggtgcc agcgcgatcg tgtcctgggc gaagacgttt gccaggttca
      181 gcgtcgcccg cggcggttcg tagcgcaggc tgctcgtgct ggcgaagtgg atctggtaat
      241 cgaccacgcg ctcgccggcg ccccagacga tgctgttcca gccggcgaac ttgaagctgt
      301 gctggatttc catgtcgtag gtattgaccg cgaagccaag cccattgttc gaccagcgtt
      361 gcgcgttgtc ataataggcc agcagctgca gatgtgaacc gccactgagt tcgcggttgt
      421 aggacaccat cgcgtcgccg ccgcggacgg tgccgcccgg ctgttccgtc gcgtcgaaga
      481 aatcccccag cagcgacagt gattgccccg gcgggttcca gtcgaggcgg aagccgccct
      541 gcggcttgta ccacgcatcg cccgcgttgc cgccgccggg agcgggaaat gcattgaaat
      601 gggtgaattc gccatgcacg cgataggtca gcgtcgggct gagccggccg ccgtactcaa
      661 tcgcatcgta cagctcgtaa ttgccgccgc cgaccgtgag caaaccgcct tgggtatcgc
      721 tggaattccg ggtgatgatg ttgatgacgc cgtttaccgc gttggcgccc cacaacgtgg
      781 cgcccggccc gctgatgacc tcgatacgct cgatatcctg cggctgaacg ccgatcatgt
      841 cccaatacac gccaccgaac agcggcgtgt acacgctgcg cccatcgatc agcaccagca
      901 gcttgttcga caggatggcg ttgttgccgg cattgaagcc gcgcgcggaa acctcgtagc
      961 cgctggaatt gacttgcgcc acgttcaggt tcggcgccag gcgcagcatc tcggggatcg
     1021 tcgttgcgcc cgaatcgacg atctcctcgc gggtgatgac atagatcgcg gcgggcgcat
     1081 cggcgagcgg ctggcgccgc ttctccaccg aggtcacttg cagctggctg agctgctcga
     1141 tggaaagccg ctgcaattgc tgcagttcaa gctgcgccat ctgttgcgcc tgtgccggcg
     1201 ccgccactgt gcacaggcac gcaacggcag caccggcagc gcccgtacac cactgtaccg
     1261 gtcgccggcc tcgcaccgcg gacggtggcc tcggcgtcat gcgctgaccg cgatcagccg
     1321 cacttgtttc acgtgttcgt gaatgttgcc cctcccgccg agaagtcgag ccgaatctac
     1381 ggtgtaggca gggccttaca gatgattctt caaaacgaca agcggtgtgc accgcggtac
     1441 acgaaagcgt ggcgggaatt tccggcaggg cgcgccggtc ctcgcgtgca gccgccgcag
     1501 cagcccgtga acgtggccag ttgtcgcgaa acatgagaca gttgcggcag cccaaccgac
     1561 tcacggtccg ggcaagaaca gggaaggagc ggacacatgc cggttgctgc gggactgctg
     1621 gcgggggcct tggccgggct tgcgtttgtc ggatgcggga tgaccagcgc ggcgccgcct
     1681 ccggcgcaga cacggcggcg ggaagtcgtg atcaatggca agcgcatcaa gacagtggac
     1741 gtgcatgcgc actgcatcgt ccccgcggcc gccgcattga tcagtcatcc gctcgaggcg
     1801 ccgggcctgc tgatgcacga tacaagcacc cgcatcgccg ccatggatgc ccaggggatc
     1861 gacgtcgagg cgctgagcat caatccgtac tggtaccgcg ccgaacgcga cgccgcggcg
     1921 gagctgatca ggatccagaa cgagaccctg gtggaattct gcgccgccaa tccggagcgc
     1981 ttcgtcgcct tcgccacggc gtcgctgcaa taccccgatc tcgccgcgca gcaggtggaa
     2041 cacgcggtga agcaactcgg cttccgcggc gtcggcgtcg ccggcagcgt ggcgggcgag
     2101 gagctcgcca acccgaaatt ccacccgttc tgggccaagt gcgaagagct cggcgtgctg
     2161 gtcttcctgc atccgctggg cacccgcgaa ctggagccga gcggaaggct ggggggtagc
     2221 gggctgctca ccaacacgat cggcaatccg ctggagacca cgatcgcgct gtcgcacctg
     2281 atcttcgaag gcacgctgga ccgcttccct ggcctgaaga tctgtgccgc gcatggcggc
     2341 ggcttcctgc cgtcctacgc caaccgctct gacgcggtga cccgctgctt ccccgaccgc
     2401 gtcggcccgc tgcccaagaa gccgcccacc gcctatctgc gcgacggcca gttgttcttc
     2461 gacaccatcg tgttcacccc cgaggcgctg cgccacctga tcgccgaaac cggctccagc
     2521 cagatcatgg tcggtaccga ttacccgttc ccctggacca gcaccgaggt cgatctggtg
     2581 ctgaacacgc ccggcctgac cgacgacgaa cgcgtcgcca tcctgggcgg cacggcgtcc
     2641 cgcctgctgg gcatctgacc gcgtcaccct ctccgtcccg gcgcggagag ggagccccgc
     2701 acgcggcctt gtgcgggaac tcggccggtg cctgacacgt tcggcccgcg ccatgatcag
     2761 ggctgcgacc aagcttctgc tgctgttcgg gctgtatgtg acgctctccg gccagggcag
     2821 cccggatgag ctgaccgcgg cagggctgtg cgcccttgcc gccgccgcct tggctgtcac
     2881 cattccccgc atcgccgaac gtcacttcgc cttccggggc gtgccctggc tgcgcttggg
     2941 cgcgggcacg gcggcgtcgc tggccggtga cctggtccgg gtggggctgc gcctggcgcg
     3001 gcccgccatc ccgcccggct ccttccatcg ctggcccttc gccgccggtg gcgaagggcg
     3061 gcgcgactcc gcccgccgcg gcctggtcac ggtggtggct tccctggcgc ccaacaccta
     3121 tgtcgtggcg gtgctgatcg gacggcgcga attgctggtg caccggctgg cgccggcacc
     3181 gccgccgcgt gaccgggact ggccgcagtg aacccctggc tcgccgcggt cgtggccttg
     3241 ctgccggcgc tggcggcgcc gctgtggatc gcgctgcgcg ggtcgctggc agaccgtctg
     3301 gtcgcggtcc aactggccgg caccatgacc acggtgatca tgacgctgat gagtttcgcg
     3361 ttcgaccagc cgtcctggat cgacctgtcg ctgaccctgg ccttcctggc cttgcccggc
     3421 acgctggtgc tggcgatctt cgtggagcgc tggatgtgat cgccgtatcg ctgctgctcg
     3481 ccgtgctggt cctgctgttg tggctcgccg cgtggggttt cgcccgcctg cgcacgccat
     3541 acgaccggat ccattgcacc agcttcgcta cgctcgccgc cgcggtgccc ctggtgctgg
     3601 tcgccttcgt gtcagacggc gcctccgacc gcgcctgcaa gatactgttg ctggccgcgc
     3661 tgatgctggt gtccggcgca acgctggcgc atgcgaccgg ccgggcgatc g
//
LOCUS       DASSZH010000687         3938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1465748, whole genome shotgun sequence.
ACCESSION   DASSZH010000687 DASSZH010000000
VERSION     DASSZH010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1465748"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..860)
                     /gene="dnaQ"
                     /locus_tag="VFN42_14835"
     CDS             complement(132..860)
                     /gene="dnaQ"
                     /locus_tag="VFN42_14835"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HET8997943.1"
                     /translation="MGEALQAMAGASAWDGAGERSVLFDTETTGLEPLQGHRIIEVAA
                     LELVNDLPTGRYFHTLIDPERDVPEDASRIHGFTNAHLAGKPRFAEIIDEMLAFFGDG
                     RLIAHNAPFDFGFLDAELARAGRPALQRERMVCTLVLAKGRFPGMPNSLDALCRRFNI
                     DLSARTTHNALLDCKLLAEVYVELTGGRQRGLSLAAETAALPMVAYSHAGPRTPRPIV
                     PNEAELAAHAAFLDRLKEPIWQAG"
     gene            complement(947..1534)
                     /gene="coaE"
                     /locus_tag="VFN42_14840"
     CDS             complement(947..1534)
                     /gene="coaE"
                     /locus_tag="VFN42_14840"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040291.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET8997944.1"
                     /translation="MKVIGLTGGIGMGKSTAAAVFRRARIPVFDADAAVHRLQARGGR
                     AVRAIESAFPGTVRDGAIDRAALRQAVVGKPDALQRLEHILHPMVEREEREFVQRARR
                     HGQQVVVLDIPLMFEVGGDKRVDQVIVVSAPPSVQLHRVRLRRRMNAADIAAMIARQM
                     PDAEKRRRADLVVRTGLSRHHTLRRLRRFIRELRR"
     gene            complement(1531..2367)
                     /locus_tag="VFN42_14845"
     CDS             complement(1531..2367)
                     /locus_tag="VFN42_14845"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET8997945.1"
                     /translation="MPILTGNARVAGIIGWPVSHSRSPRLHGFWLERHGIDGAYVPLP
                     IQPEHFGLAVRGLVAAGFAGSNVTAPHKRAAFEICDRVEDVARRAGAVNTLIFRDGMI
                     TGTNTDGTGFIANLRASGVDPAAGPALLLGAGGGARAVAAALQDAGATVTVTNRTTAR
                     AEALAHDLPGLRVIPWEQRHHALRDHALVVNTTSLGMAGHDSLQLDLDGAPQQLAVAD
                     IVYVPLETPLLAAARARGLRTVEGLGMLLYQARAGFEAWFGVQPTVDDALRRFVEAGL
                     TS"
     gene            2564..3748
                     /locus_tag="VFN42_14850"
     CDS             2564..3748
                     /locus_tag="VFN42_14850"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HET8997946.1"
                     /translation="MDTLPNPSRRLLVVDDEPPQCLLVARTVARLGYATDIAMTLDEA
                     SAQLAVRRYDAVVLDLSLAEEAVNLLHALRGMAAQAALVLVSRLDERLRAASLWLASA
                     LGLRVAGALPKPIVPAALQVVLRDAPAAPASHDLRCRISAAELEDAVRQARIVAAFQP
                     QVSLADRQVVGAEALARWRHPAGDVPPDLFVPLAERSGLIVPLTFSILRQALGACRRW
                     RDTHPHCTVAVNFSPLVLADPGLPAAVERLLRENGLGPGALTAEITESTVIGHPLVAA
                     EVLRQLRDAGVRLSIDDFGTGQSSLMTLHRLPYAELKIDRAFVTGCDTDPQALKIVRA
                     TLSIAREMGLAVVAEGIETEPEEALLREAGCETGQGWRYGRAMPEAEFRAWLAGPGPH
                     LT"
     gene            complement(3756..>3938)
                     /locus_tag="VFN42_14855"
     CDS             complement(3756..>3938)
                     /locus_tag="VFN42_14855"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET8997947.1"
                     /translation="NGIRIDLKLSQRDLSNLVAASRESVNKQLGAWREEGVLSLDDGY
                     IVLRSQAGLQTLVEMP"
BASE COUNT          572 a         1381 c         1369 g          616 t
ORIGIN      
        1 gctgctcggc gatccgctgg ggccacgcca gcttgtggcg ctggccatga cgctaaccgg
       61 cgtcggactg gccgcccggg gataggtgag ggggctgtcc cggccgggcc cgcgcatcgc
      121 acgctgcagg ctcagcctgc ctgccagatc ggttccttca accggtcgag gaacgccgca
      181 tgcgccgcca gctccgcctc gttcggcact atcggccgag gcgtgcgcgg gccggcgtgg
      241 ctgtaggcca ccattggcaa cgccgctgtc tctgccgcca gcgacaggcc gcgctgccgg
      301 ccgccggtga gttccacata cacctcggcc agcagcttgc agtccagcag cgcgttgtgc
      361 gtggtgcgcg ccgacaggtc gatgttaaag cgccggcaca aggcgtccag gctgttcggc
      421 atgccgggga agcggccctt ggcgagcacc agcgtgcaga ccatgcgttc gcgctgcagc
      481 gccggccggc cggcccgtgc gagttccgcg tccaggaaac cgaaatcgaa cggcgcattg
      541 tgcgcgatca gcctgccgtc gccgaagaac gcgagcatct catcgatgat ctcggcgaac
      601 cgcggcttgc cggccagatg cgcattggtg aagccgtgga tgcgcgacgc atcctccggc
      661 acatcacgct cgggatcgat cagcgtatgg aaataccggc cggtcggcag gtcattgacc
      721 agttccaggg ctgccacctc gatgatgcga tggccttgca gcggctccag cccggtggtt
      781 tccgtatcga acagcacgga ccgctcgccg gcgccgtccc atgccgaagc gcccgccatg
      841 gcctgcaatg cctcacccat ccagcctgtt cctcgaactg cacgcttcgc ttggcgacat
      901 tatccactgc accgcgagtc gcccaagcct ggccgcgcat tcggatctat ctccgcaatt
      961 cgcggatgaa ccgccgcagc cgccgcagcg tgtggtggcg cgacaggccg gtgcgcacga
     1021 ccaggtctgc gcggcggcgt ttctcggcat ccggcatttg ccgggcgatc atcgcggcga
     1081 tatcggccgc gttcatgcgg cggcgcaagc ggacgcggtg cagttgcacg cttggcggtg
     1141 ccgaaaccac gatcacctgg tccacccgtt tgtcgccgcc cacctcgaac atcagcggga
     1201 tatccagcac caccacctgc tgcccatgcc gccgcgcgcg ctgcacgaac tcccgctcct
     1261 cccgctcgac catcgggtgc aggatgtgct ccagccgttg cagcgcatcg ggcttgccca
     1321 ccaccgcctg acgcagcgcc gcgcgatcga tcgcgccgtc gcgcaccgtg ccaggaaagg
     1381 cggactcgat cgcccggacc gcgcggccgc cgcgcgcctg caagcggtgc accgcggcgt
     1441 cggcatcgaa caccgggatg cgggcgcggc gaaacaccgc ggcggcggtg gacttgccca
     1501 tgccgatgcc gccggtgagg ccgatcacct tcatgacgtc aggccggctt ccacgaagcg
     1561 gcgcaacgca tcatccaccg tgggctgcac gccgaaccac gcttcgaacc cggcgcgcgc
     1621 ctggtagagc aacatgccca ggccttcgac ggtgcgcagg ccgcgcgcgc gggcggccgc
     1681 gagcagcggg gtttccagcg gcacatagac gatatccgcc accgccaatt gctgcggcgc
     1741 gccgtccagg tccagttgca ggctgtcgtg cccggccatg ccgagcgagg tggtgttcac
     1801 caccagcgca tgatcgcgga gcgcgtggtg gcgttgctcc cacgggatca cccgcaggcc
     1861 gggaaggtcg tgcgccagcg cctcggcgcg ggcggtcgtg cggttggtga ccgtcaccgt
     1921 tgcgcctgca tcctgcagcg ctgcggccac cgcgcgcgcg ccgccaccgg cacccagcag
     1981 caaggcggga cccgcggccg gatcgactcc gctggcccgc agattggcaa tgaagccggt
     2041 gccgtcggtg ttcgtgccgg tgatcatgcc gtcgcggaag atcagcgtgt tcactgcgcc
     2101 ggcccggcgt gcgacgtcct ccacccggtc gcagatctcg aacgcggcgc gcttgtgcgg
     2161 ggcggtcaca ttgctgccgg cgaatccggc ggccaccaag ccgcgcaccg ccagaccgaa
     2221 atgctccggt tggatcggca gcggcacata ggcgccgtcg attccgtgcc gctccagcca
     2281 gaagccatgc agccgcggcg agcgggaatg ggaaacgggc cagccgatga tgccagcgac
     2341 ccgcgcgttt ccggtgagaa tgggcatggg gcggaaccat tgctgagccg gtgggaaagt
     2401 caactgcgtt gcgcgatgcc gcgccagcgc ggtggagtct gcgggccggc gctggagtcg
     2461 gatgctcccc agctgggtgg acgaagatcc gcgatgagcc tccttcacca gttgttcatt
     2521 ccctgcctgt aagcctccgc atcccaatgt cgcggaggcc ggggtggaca cgctgccgaa
     2581 tccctcacga cggctgctgg tggtggacga tgaaccgccg caatgcctgc tcgtcgcacg
     2641 caccgtggcc cggctcggct acgccaccga catagcgatg acgctcgacg aggcgtcggc
     2701 acagcttgcc gtacgccgct atgacgccgt ggtgctggac ctctccctgg ccgaggaggc
     2761 cgtgaacctc ctgcacgcgt tgcgcgggat ggctgcgcag gccgcgctcg tgctcgtctc
     2821 ccggctggat gaacggttgc gggccgccag cctctggcta gcgtccgcgc tggggctgcg
     2881 cgtcgccggc gcgctgccga agccgatcgt gcccgcggct ttgcaggtgg tgctgcgcga
     2941 tgccccggcc gcgccggctt cgcacgatct gcgttgccgg atcagtgcgg cggaactgga
     3001 ggacgcggtt cggcaggcgc ggattgtcgc cgccttccag ccgcaggtga gcctggcgga
     3061 ccggcaggtg gtcggcgccg aggcgctggc gcgctggcgc catccggcgg gcgacgtgcc
     3121 gcccgacctg ttcgtgccgc tcgcggagcg cagcggcctg atcgtcccgc tcaccttcag
     3181 catcctccgc caggcactgg gcgcctgccg gcggtggcgc gatacgcatc cacactgcac
     3241 cgtggcggtg aacttctcgc ccctggtgct ggcggatccc gggctgccgg cagcggtcga
     3301 gcgcttgctg cgggagaacg gcctgggccc cggcgcactg acggcggaga tcaccgaaag
     3361 caccgtcatt ggccatccgc tggtcgccgc cgaggtgctg cggcagttgc gcgacgccgg
     3421 ggtgcggtta tcgatcgatg atttcggtac cggccagtcc tcgctgatga ccctgcatcg
     3481 gctgccctat gcggaactga agatcgatcg ggccttcgtc accggctgcg acaccgatcc
     3541 gcaggcgctg aagatcgtgc gggccacgct ttcgattgcg cgggagatgg gcctggcagt
     3601 ggttgccgag gggatcgaga cggaacctga ggaggcattg ctgcgcgagg cgggttgcga
     3661 gaccgggcag ggctggcgct acggccgggc catgccggaa gcggagttcc gcgcctggct
     3721 cgccgggccg ggtccccacc tgacgtgagg gtgcgtcagg gcatctccac cagcgtctgc
     3781 agcccggcct ggctgcgcag cacgatatag ccgtcatcca ggctcaacac gccctcctca
     3841 cgccaggcgc cgagctgctt gttgacgctt tcgcgcgagg cggcgaccag gttgctcagg
     3901 tcacgttgcg acagcttcag gtcgatgcgg atgccgtt
//
LOCUS       DASSZH010000688         4705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_357616, whole genome shotgun sequence.
ACCESSION   DASSZH010000688 DASSZH010000000
VERSION     DASSZH010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357616"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VFN42_14860"
     CDS             <1..210
                     /locus_tag="VFN42_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997948.1"
                     /translation="VISEFLAGGRQLNADPVTGQAAWYDLRVRVEKADGPAVTEPRPA
                     ALRHPPGLRPAPHTLHYNVGARVAE"
     gene            428..1168
                     /locus_tag="VFN42_14865"
     CDS             428..1168
                     /locus_tag="VFN42_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET8997949.1"
                     /translation="MTSIPTPLSGKKLGLVIDLDTCVGCHACAVNCKEWNTSGHSAPL
                     TDFDAYQDHPDGVWLNRVHTYEAGTGPDSRTVHFPRSCLHCEQPACVTVCPTGASYKR
                     AEDGIVLVNAEICIGCKLCSWACPYGAREFDEDEGVMRKCTLCIDRIYNEYLPEQERV
                     PACVATCPAKARHFGDLGDPDSAVSQLVAERGGFDLMPEMDYRPVNKYLPPRRNPVRE
                     PPREKQSVGTGTGTGASVLRWIDRVLSR"
     gene            1165..2115
                     /locus_tag="VFN42_14870"
     CDS             1165..2115
                     /locus_tag="VFN42_14870"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmsC/YnfH family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET8997950.1"
                     /translation="MNPQFSVVFLTTASGVGYGMLAWLGVLNAARLLPGSPWFGIAGT
                     LLGLALSAAGLLASTFHLGHPERAWRAVSQWRSSWLSREGVLSLFTNLPALGFAASWG
                     IGGGSSVATVVLGLLTALCALLTVVSQAMIYASLKPVRQWHNGVVLPVFLLISLFSGA
                     AWIAALGAFWNSSAARAAMVLALAGAVIALLVKVVYWRRIDDARTSTSIETATGLGAL
                     GPVRSLEAPHTQENYLLREMGFAIARKHAARLRRIALLVGFVIPAVLLLIGVAWSGAA
                     SVVVLVLAALIAPLGIYVERWLFFAEATHTVTLYYGRRAG"
     gene            complement(2126..3430)
                     /locus_tag="VFN42_14875"
     CDS             complement(2126..3430)
                     /locus_tag="VFN42_14875"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8997951.1"
                     /translation="MSPPPHAASESSASDAASTVRGRGTAVFAWCLYDWAYSAFNTIV
                     STFVIATYFVRAVAANPASGTAEWATAQAIAGLLIALLAAPLGAIADYGGRRRSMLAG
                     ATIIMVLATAGLWFIHPTPSDALWALLLVAIATIAYEIATVFYNAMLPDIAPPGRIGR
                     ISGLAWGSGYVGGLICLGLCLVLLINPHPPLFGLDARQAEPVRAAALLAAAWLTLFSW
                     PVLVAVPDRALRRPWRVAVRAGMRVLRTSIRHAAAQAGLFRFLIARMLYTDGLTTLFA
                     FGGIYAAGTFGMDARGVLLLGIGLNVTAGIGALAFAFLEDVFGSKPTILVALAALTLL
                     GAAVLVVHSITLFWILALGLGMFVGPAQSASRSLMARIAPTEFRNAQFGLYALSGRVT
                     GFIGPAALGAVTAATGSQRAGMAVIVLLLVTGGALLAATRMR"
     gene            complement(3581..3985)
                     /locus_tag="VFN42_14880"
     CDS             complement(3581..3985)
                     /locus_tag="VFN42_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET8997952.1"
                     /translation="MATNPSSDQLVGILRETVVALVRRDGTDLSARQLGVFLTCYLRE
                     GPHTVRGLAAELNVSKPAITRALDRLGELELARRKVDPLDRRSVLVQRTLKGAAFLRD
                     LRSIMQEAGAGTPVAPKRSSHGRGESARRVVA"
     gene            complement(4131..>4705)
                     /locus_tag="VFN42_14885"
     CDS             complement(4131..>4705)
                     /locus_tag="VFN42_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HET8997953.1"
                     /translation="EVRVGCVENSVSGTAMRPLDVIRTRKGLTVEVGNTDAEGRLVLC
                     DLLAEACEAQPALLVDYATLTGAARVALGPDLPALFCNDDAVAETIGRAGRQSHDAVW
                     RLPLWDGYDAWLNSPTADINNISSKPFAGAIIAALYLRRFVSNGTRWAHFDVYAWNDQ
                     TQPGRPEGGEAQAIRGMLQAIIDQFGILKR"
BASE COUNT          811 a         1557 c         1573 g          764 t
ORIGIN      
        1 gtgatctcgg aattcctggc cggcggccgg cagttgaacg ccgatccggt caccggccag
       61 gcggcctggt acgacctgcg cgtgcgggtg gaaaaggccg atggcccggc agtcaccgaa
      121 ccgcgtccgg ccgccttgcg ccacccgccc ggcctgcggc ccgcgccgca cacgctgcac
      181 tacaacgtcg gtgcgcgggt cgcggaatga tggcgttgca caccggctgc accaaccccg
      241 tcgtggcggg cgaaggccct ccacccacga ctttctcgca tgcaggtgag ggagatcgtg
      301 gatggccggc cttcgccggc catgacacga aggccacgac cttcgccgcc cacgacaggt
      361 cgggcacgac cttcgccgct cacgatgcgg aacctgggca ccacgccgat gcaagagtaa
      421 ccgcaccatg accagcatcc ccacgccgct cagcggcaag aagctcggcc ttgtcatcga
      481 cctggacacc tgcgtcggct gtcacgcctg cgcggtgaac tgcaaggagt ggaacacctc
      541 cggccattcc gcgccgttga ccgacttcga cgcgtatcag gatcatccgg acggcgtctg
      601 gctgaaccgg gtgcacacct acgaagccgg gaccggcccg gacagccgca cggtgcattt
      661 tccgcgttcc tgcctgcact gcgaacaacc cgcctgcgtc accgtctgtc cgaccggcgc
      721 cagctacaag cgggcggaag acggcattgt gctggtgaac gcggagatct gcatcggctg
      781 caagctgtgt tcctgggcct gcccgtacgg cgcgcgcgaa ttcgacgagg atgaaggcgt
      841 gatgcggaaa tgcacgctgt gcatcgaccg catctacaac gagtacctgc cggaacagga
      901 acgcgtaccc gcctgcgtcg cgacctgtcc ggccaaggcg cgccatttcg gtgacctggg
      961 cgatccggac agcgcggtgt cgcaactcgt ggcggagcgc ggcggcttcg acctgatgcc
     1021 ggaaatggac taccggccgg tgaacaaata tctgccgccg cgccgtaacc cggtgcgcga
     1081 accgccccgg gagaagcaga gcgtcggtac cggcaccggc accggcgctt ccgtgctgcg
     1141 ctggatcgac cgggtcctgt cgcgatgaac ccacagttct ccgtcgtctt cctgacgact
     1201 gcgtccggtg tcggctacgg catgctggcc tggctgggtg tgctgaacgc ggcgcgtctg
     1261 ctgcccggtt cgccgtggtt cggcatcgcc ggtacgctgc ttgggctggc cttgtccgcc
     1321 gctgggctgc tggcttcgac ctttcacctt gggcatccgg agcgcgcctg gcgcgcggtg
     1381 tcgcaatggc gcagttcctg gctgtcacgc gagggggtgc tgtcgctgtt caccaacctg
     1441 ccggcactcg gcttcgcggc gtcctggggc atcggcggcg gcagcagcgt cgccacggtg
     1501 gtgttgggcc tgctgacggc gctctgcgcg ctgctgaccg tggtcagcca ggcgatgatc
     1561 tacgcctcgc tgaagccggt gcggcaatgg cacaacggtg tcgtgctgcc ggtgttcctg
     1621 ctgatttcgc tgttttccgg cgccgcctgg atcgcggctc tgggcgcgtt ctggaacagc
     1681 agcgcggccc gggccgcgat ggtgttggcg ctggccgggg cggtgatcgc gttgctggtg
     1741 aaggtcgtct attggcggcg catcgacgat gcgcgcacat ccaccagcat cgagaccgca
     1801 accggcctcg gcgcgctcgg cccggtgcgt tcgctggagg cgccgcatac tcaggagaac
     1861 tacctgctgc gcgagatggg cttcgccatc gcccgcaaac acgccgcgcg gttgcggcgc
     1921 atcgcactgc tggtcggttt cgtcattccg gcagtgctgc tgttgatcgg cgtcgcctgg
     1981 agcggcgccg cgagcgtggt cgtgcttgtg cttgccgcgc tgattgcgcc gcttggcatc
     2041 tacgtggaac gctggctttt cttcgccgaa gccacccata cggtgacgct gtattacggc
     2101 aggcgcgcgg gatagaagcg cgccgctacc gcatccgcgt cgcggcgagc agagccccgc
     2161 cggtaaccag caacaggacg atcaccgcca tgccggcgcg ctggctgccg gtggccgcgg
     2221 tcaccgcgcc cagcgcggcc ggcccgatga aaccggtcac ccggccggac agcgcgtaaa
     2281 gcccaaactg cgcattgcgg aactccgtgg gtgcgatgcg tgccatcagg ctgcggctgg
     2341 cggattgcgc gggtccgaca aacatgccca ggcccagcgc gaggatccag aacagcgtga
     2401 tgctgtgcac cacaagcacc gccgcaccca gcagggtcag cgcagccagc gcgaccagga
     2461 tggtaggctt ggagccgaac acatcttcca ggaaggcgaa ggcgagggcg ccgatgccgg
     2521 cggtgacgtt caagccgatg cccagcagca gcacgccgcg cgcatccatg ccgaaggttc
     2581 cggcggcata gatgccgccg aaggcgaaca gcgtggtcag tccatcggtg tagagcatcc
     2641 gggcgatcag gaagcggaac agccccgctt gtgcggcggc gtgccggatg ctggtacgca
     2701 gcacgcgcat gccggcgcgc acggcgacac gccagggccg gcgcagtgca cggtcaggta
     2761 cggcgacgag cacgggccag gaaaacaacg tgagccaggc ggcagccagc aatgccgcgg
     2821 cacgcactgg ctctgcctgg cgtgcatcca gaccgaacaa aggcggatgc ggattgatca
     2881 gcaggaccag gcagagtccc aggcaaatca gtccgccgac atagcccgat ccccaggcca
     2941 ggccggagat gcggccgatc cggcccggcg gcgcgatgtc cggcagcatc gcattgtaga
     3001 agacggtggc gatctcatag gcgatggtcg caattgcaac caagagcagc gcccagagcg
     3061 catccgacgg tgtcggatgg atgaaccaca agccggccgt tgccagcacc atgatgatgg
     3121 tggcgccggc cagcatgctg cggcggcgtc cgccgtaatc ggcgattgca cccaatggcg
     3181 cggccagcag tgcgatcaac aggccggcga tcgcttgcgc ggtggcccat tcagcggtgc
     3241 cgctggcagg atttgccgcc acggcgcgca cgaaataggt ggcgatgacg aaggtggaga
     3301 cgatggtgtt gaaggcgctg tacgcccagt catacaggca ccaggcgaaa acggcggtgc
     3361 cgcgtccgcg cacggtggaa gctgcatccg aagcagaaga ttcggacgcc gcatggggag
     3421 gaggtgacac ggtgcgggga cttctccggt ttcaccggca ttcaaacacg acaaaaccgc
     3481 cctgaccaag catcaaggcg gttccatgta ggcaaccagg caaccaaaga ccgcgggcaa
     3541 cgaaccgcgg tccggtccag tcagggcgat gcggtcgggg tcaggcgacg acgcggcgtg
     3601 cgctttcgcc ccggccatga ctgctgcgct tcggcgccac cggcgtgccg gcgccggctt
     3661 cctgcatgat gctgcgcaga tcgcggagga atgcggcgcc cttcagggtc cgctgcacca
     3721 gcacgctgcg gcggtccagc gggtcgactt tgcggcgggc cagttccagc tcgcccagcc
     3781 ggtccagcgc gcgggtgatt gctggcttcg acacgttgag ttccgctgcc aagccgcgca
     3841 cggtgtgtgg accttcgcgc aggtagcagg tcaggaacac gccgagctga cgggcagaca
     3901 gatcggtgcc gtcccggcgg accagcgcaa caacggtttc gcgtaagata ccgacaagct
     3961 ggtcggagga cggattcgtt gccatttttt aaccctttca tcgtcacacg agtcatgtgg
     4021 atcgactcgt ccccgatgcg ccgcttaaac ctgcagacaa tccgccgtca actttatttc
     4081 agttcacgaa ctccgtccaa ccgcaaactc acccagtcgt gaggaattgt tcatctcttt
     4141 aagatgccga attgatcgat gattgcctgc agcatgccgc gtatggcctg cgcttcgccg
     4201 ccctccggcc ggcctggctg cgtctgatcg ttccatgcgt aaacatcgaa atgggcccat
     4261 ctggtaccat ttgaaacgaa gcgccggaga tataacgctg caatgattgc gccagcgaac
     4321 ggctttgacg aaatattgtt gatatccgcc gtcgggctgt tcagccaggc gtcatagcca
     4381 tcccacagtg gcagccgcca gaccgcatcg tgcgattgcc ggccggcccg accaatggtt
     4441 tcagccaccg catcatcgtt gcagaacagt gccggcaggt ccgggcccag cgcgacgcgc
     4501 gccgcgccgg tcagcgtcgc gtagtcgacc agcagcgcag gttgcgcttc gcaggcttcg
     4561 gcgagcaggt cgcacagcac cagccggccc tcggcgtcgg tgttgccgac ttccaccgtc
     4621 aatcccttgc gcgtgcggat cacgtcgagc gggcgcattg ccgtgccgga taccgagttt
     4681 tccacgcagc cgacgcggac ctcca
//
LOCUS       DASSZH010000689         5157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1568052, whole genome shotgun sequence.
ACCESSION   DASSZH010000689 DASSZH010000000
VERSION     DASSZH010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1568052"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="VFN42_14890"
     CDS             complement(<1..962)
                     /locus_tag="VFN42_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET8997954.1"
                     /translation="MDIGSLPAPGVPTQWRPDGCAHVAMVLQGGGALGAYQAGVYEAL
                     HEAGLAPNWIAGVSIGGINGAIIAGNRSENRLPRLREFWNLVTSRPDWPFPLDGDDAR
                     KMHNGWSSLLTVALGQPGFFSPRTPSPWLAPRGGRGATSIYDTAQLRETLLRLVDFDL
                     LNRRAVRYACGAVNVASGNFAYFDNSSSEILPEHVMASGALPPGLPMVRVGTDWYWDG
                     GIVSNTPLQHLIDHVGDDSALVFQVDLFSARGPIPRDMAEVLGREKDIRYSSRTRLTT
                     DRYLQVCRLERQVRSLLARVPDEALTAQERALKEKLLTLPRLSIL"
     gene            1089..1874
                     /locus_tag="VFN42_14895"
     CDS             1089..1874
                     /locus_tag="VFN42_14895"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HET8997955.1"
                     /translation="MSLKGKVALVTGSTSGIGLGIAQALAAQGADVMMNGFGDPAEIE
                     RLRTQLATEFGVRVAYSDADISKPAAIREMVAQAGRTLGSLDILVNNAGIQFTSTIED
                     FPDEQWDRVIAINLSGVFHATKAAVPLMKQRGWGRIINIASAHGLVASGQKAAYVTAK
                     HGVLGLTKVTAIDMANTGITANAICPGWVLTPLVQKQLDDRATREGRSVEEVTHSLLA
                     EKQPQTSFTTPEQIGGLAVFLCSDAAANMTGVPLSIDGGWTAQ"
     gene            complement(1880..3274)
                     /gene="cysG"
                     /locus_tag="VFN42_14900"
     CDS             complement(1880..3274)
                     /gene="cysG"
                     /locus_tag="VFN42_14900"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HET8997956.1"
                     /translation="MAVVRPCPAPYLPAISAGPARMQHFPVFLDLKDRPVLVLGSGEI
                     AARKADLLRRAGAAVHASGHFTPDLLTGCALAVGAGAPEADLQALAAAARAAGIPCNV
                     VDRPELCSFIMPAVIERDPITIAVSSAGVAPVLARLLRARIEAAVPPGFGRLAALAAR
                     FRDEIRRRLPDLATRRRVLERLFTGPAADLVFAGREAEAEAAFARALDTGDAAEGIVF
                     LVGGGPGAADLLTLRAQRLLGEADVIVHDRLVGDAVLDLARRDAARIYVGKVRAKHCL
                     PQEGINDLLIRLAREGRRVVRLKGGDPFIFGRGGEEAEALAAAGVRCEVVPGVTAALA
                     CAAQEMIPLTHRGVARSVTFVTGHTREGRLDIDFGPLVRARTTLAVYMGIVSLPQLRD
                     GLLAHGLDPATPAALIERGGTPQHRALRGTLDELVARAPDWSTGGPALVLVGEAVARG
                     GTRTPAAQATPAPA"
     gene            3346..4344
                     /locus_tag="VFN42_14905"
     CDS             3346..4344
                     /locus_tag="VFN42_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET8997957.1"
                     /translation="MPILLTGAAGFIGYHVAEALLARGESVLGLDNLSAYYDVRLKQA
                     RLARLQGRPGFTFRRCDLADRAAMTDLAAANRDVTRIVHLAAQAGVRHSLADPYAYVA
                     SNVTGHLAVLEMARHLPRCEHLVYASSSSVYGANTKLPFAESDRVDTPLSLYAATKRA
                     DELMSHAYGHLFALPQTGLRFFTVYGPWGRPDMAYFAFAEAILRGEPITVYDEGRVRR
                     DFTYIDDIVAGILGCLDRPPAGESPPLRLLNIGNNRSEPVSALIALLEEALGRSAIIR
                     AAPRPLADVAETFADVAAIGALTGFRPATPLAVGIPRFASWFLGWQANRDGAKKTP"
     gene            4766..>5157
                     /locus_tag="VFN42_14910"
     rRNA            4766..>5157
                     /locus_tag="VFN42_14910"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          814 a         1705 c         1864 g          774 t
ORIGIN      
        1 aggatggaaa gccgcggcag ggtaagcaat ttctccttca gcgcacgctc ctgcgcggtc
       61 agcgcctcgt ccggcacgcg ggcgagcagg ctgcgcacct ggcgttccag ccggcagacc
      121 tgcaggtagc ggtcggtggt cagccgcgtg cggctggaat agcggatgtc cttctcgcgc
      181 ccgagcacct cggccatgtc gcgggggatc ggcccgcggg cgctgaacag gtccacctgg
      241 aacaccagcg cgctgtcgtc gccgacatgg tcgatcagat gctgcagcgg tgtgttggag
      301 acgatgccgc cgtcccagta ccaatcggtg ccgacccgca ccatcggcag ccccggcggc
      361 agggcgccgc tggccatgac atgctcgggc aggatttccg acgacgaatt gtcgaaatag
      421 gcgaagttgc cgctggcgac attgaccgcc ccgcaggcat agcggacggc gcggcggttc
      481 agcaggtcga agtccaccag ccgcagcagc gtctcgcgca gctgtgcggt gtcgtagatg
      541 gaggtggcgc cccgcccgcc gcgcggggca agccaggggc tcggcgtgcg cggcgagaag
      601 aaccccggct ggcccagcgc gacggtcagc agcgacgacc agccgttatg cattttgcgc
      661 gcatcgtctc cgtccagcgg gaacggccag tccggccggc tggtcacgag gttccagaac
      721 tcgcgcaggc ggggcaggcg gttctccgac cggttgccgg cgatgatggc gccgttgatg
      781 ccgccgatgg agacgccggc gatccagttc ggcgccagcc cggcctcgtg cagcgcctca
      841 tagacgccgg cctgataggc gccgagcgca ccgccgccct gcagcaccat ggcgacatgg
      901 gcgcagccgt ccggccgcca ctgggtggga acgcccgggg cgggcaggga gccgatatcc
      961 atgcgggaag gtccgttcgt cgtttcgccc taccagcgtg gcgccattgc ccccgacgtc
     1021 aagggggcgg cttgatccgg aggtgtcttg cggcctatgc ggcggtgaaa gcacgggaga
     1081 gacgggcgat gagcctgaag ggcaaggttg cgctggtgac gggttcgacc agcggcatcg
     1141 gtctcggcat cgcgcaggca ctggccgcgc agggcgcgga cgtgatgatg aacggcttcg
     1201 gcgatccggc cgagatcgag cggctgcgca cgcagctcgc gaccgaattc ggcgtgcgcg
     1261 tcgcctattc cgacgccgat atcagcaagc ccgccgcgat ccgcgagatg gtggcgcagg
     1321 cgggacgcac gctcggttcg ctggacatcc tggtgaacaa cgccggcatc cagttcacca
     1381 gcacgatcga ggacttcccg gacgagcagt gggaccgcgt gatcgcgatc aatctctccg
     1441 gcgtgttcca cgcgaccaag gcggcggttc cgctgatgaa gcagcgcggc tgggggcgga
     1501 tcatcaacat cgcctcggcg cacggcctgg tcgcgagcgg gcagaaggcg gcctatgtca
     1561 cggccaagca cggtgtcctc ggcctgacca aggtgacggc gatcgacatg gcgaacaccg
     1621 gcatcaccgc caacgcgatc tgccccggct gggtgctgac gcccctggtg cagaagcagc
     1681 tcgatgaccg cgcgacgcga gaggggcgca gcgtcgagga ggtgacgcac agcctgctgg
     1741 cggagaagca gccgcagaca agcttcacca cgcccgagca gatcggcggg ctggcggtgt
     1801 tcctgtgctc cgacgcggcg gcgaacatga ccggcgtgcc gctgtccatc gacggcggct
     1861 ggacggcgca gtagccgcct caggcgggcg caggcgtggc ctgcgcggcg ggcgtcctcg
     1921 tcccgccgcg cgcgaccgcc tcgccgacca gaaccagggc ggggccgccc gtgctccagt
     1981 ccggggcgcg ggcgaccagt tcgtccagcg tgccgcgcag cgcgcgatgc tgcggcgtgc
     2041 caccgcgctc gatcagcgca gcgggcgtcg cgggatcgag cccatgcgcc agcagcccgt
     2101 cgcgcagttg cggcagcgag acgatgccca tgtagaccgc gagcgtggtg cgggcgcgca
     2161 ccagtggccc gaagtcgatg tcgagcctgc cttcgcgcgt atggccggtg acgaaggtga
     2221 cgctgcgggc gacgccgcga tgggtcagcg ggatcatctc ctgggccgcg caggccagcg
     2281 ccgcggtgac gcccggcacc acctcgcagc gcacgccggc cgcggccagc gcctccgcct
     2341 cctcgccgcc acggccgaag atgaaggggt cgccgccctt cagccgcacc acgcgcctgc
     2401 cctcccgcgc caggcggatc aggaggtcgt tgatgccctc ctgcggcagg cagtgtttgg
     2461 cgcggacttt cccgacatag atgcgcgcgg catcgcgtcg ggcgaggtcg agcacggcgt
     2521 cgccgaccag ccggtcatgc acgatcacgt ccgcctcacc gagcaggcgc tgcgcgcgca
     2581 gggtcagcag gtcggccgcc cccggcccgc cgccgaccag gaacacgatg ccctcggcgg
     2641 cgtcacccgt gtcgagcgcg cgggcgaatg cggcctcggc ctccgcctcc cggccggcga
     2701 agacgagatc ggcggccggc ccggtgaaca gccgctccag cacccgccgc cgcgtcgcga
     2761 gatcgggcag gcggcggcgg atttcgtctc ggaaccgggc cgcgagggcg gccagacggc
     2821 cgaagcccgg cggcaccgcg gcctcgatcc gcgcccgcag caggcgggcc agcaccggcg
     2881 cgaccccggc cgaggagacg gcgatggtga tcgggtcgcg ttcgatcacg gcaggcatga
     2941 tgaagctgca cagctccggc cggtcgacca cgttgcacgg gatgccggcg gcccgcgccg
     3001 ccgcggcgag cgcctgcaga tcggcttccg gtgcccccgc gccgaccgcg agcgcgcagc
     3061 cggtcagcag gtcgggcgtg aaatgcccgg aggcgtggac ggcagccccg gcgcggcgca
     3121 gcaggtcggc cttgcgcgcg gcgatctcgc cgctgccgag caccagcacc gggcggtcct
     3181 tgaggtcgag aaagacggga aaatgctgca tccgagcggg tccggcggag atcgcgggca
     3241 gatagggcgc ggggcagggg cgcacaaccg ccatctcccc gcatcacgct tgctcccgcg
     3301 cgtggacgcc tcctagatgc ccgcgcaaca gtgccgggta tgcccatgcc gatcctgctg
     3361 accggcgcgg ccgggttcat cggctatcac gtggccgagg cgttgctggc gcgtggcgaa
     3421 tcggtgctcg gtctcgacaa cctcagcgcc tactacgacg tgcggctgaa acaggcgcgg
     3481 ctggcgcggc tgcaggggcg acccggcttc accttccgtc gctgcgacct tgccgatcgt
     3541 gcggccatga cggacctcgc cgccgcgaac cgggacgtga cgcgcatcgt gcatctggcg
     3601 gcgcaggccg gcgtgcggca ttcgctggcc gatccctacg cctacgtcgc ctcgaacgtg
     3661 acggggcacc tcgcggtgct ggagatggcg cgccacctgc cgcgctgcga acatctcgtc
     3721 tacgccagtt cgtcctcggt ctatggcgcc aacacaaagc tgccattcgc ggaaagcgac
     3781 cgcgtggaca cgccgctgtc gctctacgcc gccaccaagc gcgccgacga actgatgagc
     3841 cacgcctacg gccatctgtt cgcgttgccg cagaccggct tgcggttctt caccgtctac
     3901 gggccctggg ggcggccgga catggcatat ttcgccttcg cggaggcgat cctgcgcggc
     3961 gaaccgatca ccgtctacga cgaggggagg gtgcggcgcg acttcaccta catcgacgat
     4021 atcgtcgccg gcatcctcgg ctgcctggac cggccgccgg ccggcgaatc gccgccgctg
     4081 cggctgctga acatcggcaa caaccgcagc gagccggtct cggcgctgat cgccctgcta
     4141 gaggaggcgc tgggccggag cgccatcatc cgggcggcgc cgcggccgct ggcggatgtg
     4201 gcggaaacct tcgcggatgt ggcggcaatc ggcgcgctga ccggatttcg gccggcaacg
     4261 ccactggccg tcggcatccc gcgcttcgcg tcctggttcc tcggctggca ggcaaatcgc
     4321 gacggcgcga aaaaaacccc ttgaccaccc cccgccagag gactaaatcg ccgctccacc
     4381 gaacgggtgg tgcttgaact tctggcgctg ccccgttact ctcctcgact tccgctgagc
     4441 ccagcgagtc ggtggaccga taagcgaccc gagagggaag cggatcgggt cgttgttcat
     4501 tgacagtaga atctggatgg aagggatacg ttggcggcgc tccgcgactg aggtcatgtg
     4561 ccttggtgtt tgggttaggt tctggttggt ttggtatgga cctggctttg tgggcgtttg
     4621 ttggcgtgtc ttgaagatgc gttatgacag atgttccgaa tgcttgtttg gtctggggga
     4681 tctgagaggg tttcctgggc tgggtgagag agtgggttgg gtctgtgttc atctggtgtt
     4741 gtcgtgtgag cggcgatgct gggatgaacc tgagagtttg atcctggctc agagcgaacg
     4801 ctggcggcat gcttaacaca tgcaagtcgc acggtcgggg caacccggca gtggcggacg
     4861 ggtgagtaac gcgtaggtat ctatccatgg gtgggggata accttgggaa actgaggcta
     4921 ataccgcatg acacctgagg gtcaaaggcg cgagtcgcct gtggaggagc ctgcgtccga
     4981 ttagctagtt ggtggggtaa aggcctacca aggcgtcgat cggtagctgg tctgagagga
     5041 tgatcagcca cactgggact gagacacggc ccagactcct acgggaggca gcagtgggga
     5101 atattggaca atgggcgcaa gcctgatcca gcaatgccgc gtgggtgaag aaggtct
//
LOCUS       DASSZH010000690         2738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_306638, whole genome shotgun sequence.
ACCESSION   DASSZH010000690 DASSZH010000000
VERSION     DASSZH010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306638"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VFN42_14915"
     CDS             <1..446
                     /locus_tag="VFN42_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997958.1"
                     /translation="AAHPEVHAFVRWRFEPVTSLAAGIRQAAHPSSRIHVLDIGNGWL
                     CGSDPGALARATDGVVMCVYDRSPDAVGADLAATRAVIGADRFLGTGMRVFYPEMRGP
                     DDLVVRARAAKAAGVEEINFYNYGLVPAARLDWVRAAGAAVHPRR"
     gene            complement(431..1336)
                     /locus_tag="VFN42_14920"
     CDS             complement(431..1336)
                     /locus_tag="VFN42_14920"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8997959.1"
                     /translation="MYSGPIIDAHTHLWDLSMNKHAWLMPSEGAPQALGGLEAIRRNF
                     LVEDYLRDSAAQNIAASVHIEALWDPADPLGETRWLETRDKSRGVAVRYTAAAPLGTP
                     AAASVLEAQASLSRVVGIRAVVSFHPTWPEASFARHGDIATDPAWRRDVARLVKLQLN
                     LELMMYPYQLGPVLDLAHSLPDLQIIVNHCASPIDRDQDGMRRWRDAVRQLAERPNIA
                     LKVSNITAYDPQATYEGKRSVAMHCIECFGTRRTMFATDWPVAGINTTFDGIYDTFRR
                     ITADMSAGEQRDMFHDNATRFYRLG"
     gene            complement(1390..2277)
                     /locus_tag="VFN42_14925"
     CDS             complement(1390..2277)
                     /locus_tag="VFN42_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET8997960.1"
                     /translation="MRRTPLIAARHALRPATPAELWLKLECLQVTGSFKARGAMNRLL
                     TTPAEALQRGIVTASGGNHGVAVARAGHIAGVKTTVFLPANASPLKRRKLAAWGAEVR
                     IDGQVWDESDRAARAFAQAEGATYFHPFADPAVVAGQGTVALEILDELPDSTLYLIAI
                     GGGGLAAGMATALRALRPQARLIGIEPVGAPTLHASLAAGGVVTLPRVTTTVPTMACG
                     RTDERIYDIIQRTLDDIVLIEDAAMLDAASWLWFEMGIAADPSGAAAVAALRSGAVVP
                     RSGERVCALVCGAGSEGLG"
BASE COUNT          409 a          967 c          929 g          433 t
ORIGIN      
        1 tcgccgcgca ccccgaggtg catgcctttg tccgctggcg gttcgagccg gtgaccagcc
       61 tcgccgccgg gatcaggcag gcggcgcatc cgtccagccg catccatgtg ctcgacatcg
      121 gcaatggctg gctgtgcggc agcgatcccg gcgcgctggc ccgcgccacc gacggcgtgg
      181 tgatgtgcgt ctatgatcgc agcccggacg cggtcggcgc cgatctggcc gcgacccgtg
      241 ccgtgatcgg cgcggaccgg tttctcggga cggggatgcg cgtgttctat ccggaaatgc
      301 gcgggccgga cgatctcgtc gtccgggcgc gggcggccaa ggcggcgggc gtcgaggaaa
      361 tcaacttcta caattacggt ctggtcccgg cggcacgcct tgactgggtg cgcgcggccg
      421 gcgcggcggt tcatcccagg cggtagaagc gcgtcgcgtt gtcgtggaac atgtcccgct
      481 gctcgccggc ggacatgtcc gcggtgatgc gccggaaggt gtcgtagatc ccgtcgaacg
      541 tcgtgttgat cccggccacc ggccagtcgg tcgcaaacat cgtgcgccgc gtgccgaagc
      601 actcgatgca gtgcatcgcc acgctgcgtt tgccctcgta ggtcgcctgc gggtcatagg
      661 cggtgatgtt cgagaccttg agcgcgatgt tcggccgttc cgccagttgc cgcaccgcgt
      721 cccgccagcg ccgcatgccg tcctggtcgc gatcgatcgg actggcgcag tggttcacga
      781 tgatctgcag atcgggcagg ctgtgggcca gatcgagcac gggcccgagc tgatacggat
      841 acatcatcag ttccaggttg agttgcagct tcaccagcct ggccacgtcc cgtcgccacg
      901 ccggatcggt cgcgatgtcg ccgtggcggg cgaaggacgc ctccggccag gttggatgga
      961 agctcaccac cgcgcggatg ccgaccacgc gggacaacga tgcctgcgcc tccagcaccg
     1021 atgccgcggc gggcgttccc aggggggctg cggcggtata gcgcacggcc acgccgcgcg
     1081 acttgtcccg tgtctccagc caccgcgttt cgccgagcgg atcggcgggg tcccagagcg
     1141 cctcgatatg cacgctcgcc gcgatgttct gtgccgcgct gtcgcgcaga tagtcctcca
     1201 ccagaaagtt gcggcggatc gcctcaagcc ctccgagggc ctgcggcgcc ccttcggacg
     1261 gcatgagcca ggcgtgcttg ttcatcgaaa gatcccacag atgcgtatgg gcgtcgatga
     1321 tcgggccgga atacatggat cctccctcga atgaaccagc ggctcacgcc gcctgcctgc
     1381 gcgcgcgccc taccccagcc cctccgaccc cgcgccgcat acgagggcgc agacgcgttc
     1441 gccgctgcgc ggcaccaccg ccccgctgcg cagggcggca accgccgcgg cgccgctggg
     1501 gtcggcggcg atccccatct cgaaccacag ccagctggcg gcgtcgagca tggctgcgtc
     1561 ctcgatcagc acgatgtcgt ccaaagtccg ctgaatgatg tcgtagatgc gctcgtcggt
     1621 ccggccgcat gccatcgtcg ggacggtcgt cgtcacccgt ggcagcgtga ccacgccccc
     1681 cgccgcgaga gaggcgtgca gcgtcggcgc tcccaccggc tcgatgccga tcaggcgggc
     1741 ctgcggacgc aaagcccgca gggcggtcgc catgccggcc gcaaggcccc cgcctccgat
     1801 cgcgatgagg tagagcgtcg aatccggcaa ctcgtccaga atttccagcg ccaccgtgcc
     1861 ctggccggcg acgaccgccg ggtcggcgaa cggatggaaa tacgtggccc cctccgcctg
     1921 cgcgaaggca cgggcggccc gatccgattc gtcccacacc tgaccgtcga tgcggacctc
     1981 cgccccccat gccgccagct tgcgccgctt cagcggtgag gcgttggcag gcaggaacac
     2041 cgtggtcttc acgccggcaa tatggccggc acgcgcgacg gcgaccccgt gattgccgcc
     2101 cgaagcggtg acgatgccgc gctgcagggc ctctgccggc gtggtgagca ggcggttcat
     2161 ggcgccgcgc gccttgaagg aacccgtgac ctgaaggcat tccagcttca gccacagctc
     2221 tgccggcgtc gccggccgca gggcatggcg cgcagcgatc agcggcgtgc ggcgcacatg
     2281 cggcgcaatc cgcgaagcgg ccgaacgcac atcgtccagg gagatcatgt cgctgcgccc
     2341 gccaccggca cgtcgaaccg gaccgtggat gacggcaggc ggttgcggcc tgtctgcaac
     2401 cgattcatgt gtaccctccg tggtgtcctg gtggcgccgc atgatggcat tgcgccgcat
     2461 tcctgcgcca tcacgaacga ccggcgcgga ttgccacacc gccatccgcc gctcgcgcgc
     2521 gcccgtcctg gccgagcctg cggcccgttc ctgcttgaca ggctttcggg tcgactatca
     2581 aatgtcaaca ccctgtttcg tcaatgatag cgtgccgcgc cggcgctccg cacgatcgca
     2641 gggcggagtg ggccaggagg aagcaagtgt cggaactcga tcttgcccag gcatggaagc
     2701 tgccgagccg gccacggccg atcaccatca tcggtgcc
//
LOCUS       DASSZH010000691         2914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1277969, whole genome shotgun sequence.
ACCESSION   DASSZH010000691 DASSZH010000000
VERSION     DASSZH010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277969"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="VFN42_14930"
     CDS             complement(<1..1119)
                     /locus_tag="VFN42_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997961.1"
                     /translation="MVPTYINGCFAILHPAQGTRGALICGPLSDEALNAYRALVFLGE
                     HLAGAGVPTLRLAYHGTGDSAGSDDEPDHFGRWLDSIEAGVNWLRSQCGVTSVTLIGH
                     RIGASLAARAACGLDCVDSLVLLSPIGGRQFIHELTLAARVSQRVWKTSHKLDDGTWF
                     ESHGLRIGHATRDALMRLDIRKLPNLPAEHVLVLDSANRPGSQVLAGALRDSGAAARF
                     EAFDRLDAMQRDSYEAEVPHTAFIRIRDWMTGLPARCGSPRPPCVTGALVLEAAREMP
                     VRFGEDDRLFGILTLPDRLSPVAPAMLLVNTSANPRWGNARIAVDLARELAGDGIASL
                     RMDAAGMGDSDPAGGELGRPYSAAVTTDVLAAVAELEGR"
     gene            complement(1126..2151)
                     /locus_tag="VFN42_14935"
     CDS             complement(1126..2151)
                     /locus_tag="VFN42_14935"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8997962.1"
                     /translation="MPANGIQVFSTCPQSKEFAAGEYLPQVRRVSQWSEAAGCTGMLI
                     YTDNSIVDPWLLAQCVLQDTRALCPLVAVQPVYMLPYSAAKMVASLACLHDRRIWLNM
                     LAGGFRNDLIALGDETPHDDRYVRTTEYALLMRRLLESDEPVQFAGRYYAARGLRLTP
                     RVPEELRPGWLISGSSPAGRAAAVEIGAVAVEYPEPADRVQPPESVHERGIRVGIIAR
                     ESADAAWRVAWDRFPDDRRGRLSHALAMKTSDSSWHRTLSDLARARPADGSPYWLWPF
                     EQHRTFCPYLVGDYATVAQEIARYLRLGYNSFILDIPTEAADLETAAEVFRQARQLCD
                     APPPAMA"
     gene            complement(2157..2438)
                     /locus_tag="VFN42_14940"
     CDS             complement(2157..2438)
                     /locus_tag="VFN42_14940"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HET8997963.1"
                     /translation="MPDNNVVILPDEAKRLALVTEALTRRLRQEGRSMPRVALDADLI
                     ELGLVDSQALLDIILDVEQQTGCLFDAERIDFETGMTLRHLAAAFADPE"
     gene            complement(2417..>2914)
                     /locus_tag="VFN42_14945"
     CDS             complement(2417..>2914)
                     /locus_tag="VFN42_14945"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide synthetase"
                     /protein_id="HET8997964.1"
                     /translation="ELCLAGSQVTDGYWHRPDLTSQRFVRWPWDNAQRVWYRTGDRAR
                     IDPRLGLMFLGRLDRQVKIAGHRIELQEVEAVLHHAANANAAAIAWPLGPDGLARGIV
                     GFVGQSELSVETIFDACREALPPYAVPSAIHRVADWPLNSSGKTDHRQLLHIVETIAC
                     QTTTL"
BASE COUNT          504 a          991 c          970 g          449 t
ORIGIN      
        1 gcgtccttcc aactccgcga ccgccgccag cacgtcggtg gtgaccgccg cggaataggg
       61 gcggccaagt tcaccgccgg ccggatccga atcgcccatg ccagccgcgt ccatcctcag
      121 gctggcgatg ccgtcgcccg ccagttcgcg cgccagatcg accgcgatgc gtgcgttgcc
      181 ccagcgcgga ttggcgctgg tgttgaccag caacatggcc ggtgcaaccg gcgacagccg
      241 gtcggggagg gtgaggattc caaacagcct gtcatcctcg ccaaagcgaa ccggcatttc
      301 ccgcgctgcc tccagcacca gcgcccccgt cacgcagggc ggccggggcg aaccgcatcg
      361 ggcgggcagc cccgtcatcc agtcgcggat ccggatgaag gcggtatgcg gcacctccgc
      421 ctcgtacgaa tcccgctgca tggcgtcgag ccggtcgaac gcctcgaacc gtgccgccgc
      481 gccggagtcc cgcaatgcgc cggccaacac ctggctgcct gggcggttgg cggaatccag
      541 aaccagcacg tgttctgcgg gcaagttggg cagcttgcgg atgtccagcc gcatcaatgc
      601 gtcacgggtg gcgtggccga tacgcagccc atgcgactcg aaccaggtgc cgtcgtcgag
      661 cttgtggctg gtcttccaca cgcgttgcga gacacgcgcg gcgagcgtca gttcatggat
      721 gaactgccgc ccaccgatcg gcgagagcag caccagggaa tccacgcaat ccaggccgca
      781 ggccgcgcgg gccgccaggc tggccccgat ccggtggccg atcagcgtca ccgaggtcac
      841 gccgcactgc gagcgcagcc agttcacgcc ggcttcgatg ctgtccagcc agcgtccgaa
      901 gtgatccggc tcgtcatcgc tgccagcgga atcgccggtg ccatgataag ccagccgaag
      961 tgtcgggacc cccgcgccgg ccaggtgttc gcccagaaaa accagcgcgc gataggcgtt
     1021 cagggcctcg tccgacagtg ggccgcagat cagtgcaccg cgcgtgccct gggcaggatg
     1081 caagatggcg aagcagccat tgatgtaggt aggaaccatc gcgcgtcagg ccattgccgg
     1141 tggaggcgca tcgcacaact gccgcgcctg acgaaacacc tccgcggcgg tctccaggtc
     1201 ggcggcctcg gtgggaatgt ccaggatgaa gctgttgtag ccaaggcgga gatagcgggc
     1261 gatctcctgt gcgaccgtcg catagtctcc gaccaggtac gggcagaagg tgcgatgctg
     1321 ctcgaacggc cacagccagt aggggctgcc gtcggctggc cgtgcgcgcg ccagatcgga
     1381 tagcgtgcgg tgccagctcg aatcgctggt cttcatcgcc agcgcatgac tcaagcggcc
     1441 acggcggtcg tcggggaagc ggtcccaggc gacacgccag gccgcatccg cgctttcccg
     1501 cgcaatgatg ccgacgcgga tgccgcgctc atgcaccgat tccggcggct gtacgcggtc
     1561 tgccggttcc ggatattcaa ccgcgacggc accgatctcc actgccgccg cgcgacccgc
     1621 gggcgacgat ccggagatta gccagcccgg ccgcaactcc tccgggaccc tgggcgtcag
     1681 gcgcaagccg cgcgccgcgt agtagcggcc ggcaaattgc accggctcgt cactttccag
     1741 cagacggcgc atcagcagcg cgtattcggt ggtgcggaca tagcgatcgt catgcggcgt
     1801 ctcgtcgccc agcgcgatca ggtcgttgcg gaagccgccg gccagcatgt tcagccagat
     1861 acgccggtca tgcaaacacg ccagcgacgc gaccatcttc gcggcgctgt acggcagcat
     1921 gtacaccggc tgtaccgcga ccaacggaca cagcgcgcgc gtgtcctgca acacgcattg
     1981 tgccagcagc cagggatcga cgatgctgtt gtcggtatag atcagcatgc cggtgcagcc
     2041 ggcggcctcg ctccattgcg acacccggcg cacctgcggc aggtactcgc ccgccgcgaa
     2101 ctccttcgac tgcgggcagg tgctgaacac ctggatgccg ttggccggca tagccgttac
     2161 tccggatcgg cgaaggcggc ggcaaggtgc cgcagcgtca tgcctgtttc gaaatcaatg
     2221 cgctcggcat cgaacaggca accggtttgc tgttccacat cgaggatgat gtcgagcagc
     2281 gcctgcgaat ccaccagccc aagctcgatc aggtcggcat ccagggcgac gcgcggcatg
     2341 gaccggcctt cctggcgcag gcggcgggtg agtgcctcag tgaccagcgc caaccgcttg
     2401 gcttcgtccg gcaggattac aacgttgttg tctggcacgc gatagtctcc acgatgtgaa
     2461 gcaactggcg gtggtcggtc ttgccactgc tgttgagagg ccagtccgcg acgcggtgaa
     2521 tcgcggacgg cacggcgtag ggcggcagcg cttcacgaca ggcatcgaag atggtctcga
     2581 cgctaagctc ggactggccc acgaacccca cgatgccgcg cgccagcccg tcgggaccca
     2641 gcggccaggc gatggcggcg gcgttggcgt tggcggcatg gtgcagtacc gcttccacct
     2701 cctgaagctc gatacgatgg cctgcgatct tcacctggcg gtccagccgc ccgaggaaca
     2761 tcagtccgag ccgcggatca atccgtgccc ggtcgccggt gcggtaccac acgcgttgcg
     2821 cgttgtccca cggccagcga acaaaacgct gcgacgtcag gtcggggcgg tgccaatagc
     2881 cgtcagtgac ctggctgccg gcgaggcaca gttc
//
LOCUS       DASSZH010000692         5295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1073610, whole genome shotgun sequence.
ACCESSION   DASSZH010000692 DASSZH010000000
VERSION     DASSZH010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073610"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2331)
                     /locus_tag="VFN42_14950"
     CDS             complement(<1..2331)
                     /locus_tag="VFN42_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HET8997965.1"
                     /translation="MDLSLGTLAPPIPDAHRVQILRQLERKLLWLSSWMIHHANHVRP
                     NRDGLKVGGHQASCASVISIMTALYFEVARPQDRIAVKPHAGPVFHAINYLFGRQSRE
                     KLETLRQLHGIQSYPSRVKDGPEVDFSTGSVGLGVAMTTFSALMADYVRLHGLAGAEL
                     PPGRHIAIAGDAELDEGNIYEALLEGWKHDVRNLWWIVDYNRQSLDSVVPDRLFGRIE
                     GLFRDMGWNCVTIKYGRKLQAAFAQDGGEALRRWIDDCPNSLYSALTFQGGAAWRQAL
                     LADLGRSKARKIVDALDDEALGALMTNLGGHDIETVIDALRAAETDGDQPTCFIGYTI
                     KGMGLPFAGHKDNHAGLMTKEQMEGFRAQMNIRPGHEWDRFEGLDLAPEDLQAFLDSC
                     PFATKLTPAGRRLSAPVVPVPPALPLPKVAGRKVSTQSGFGEILAEIGRGQGELKALA
                     DHIMTTSPDVTVSTNLGPWVNRRGIFDRHTRNDVFRDAKLASAQRWGMSPKGQHVELG
                     IAEQNLFLLLGAAGLAPSMTGARLLPIGTVYDPFVNRGLDALIYACYQDARFLLVSTP
                     SGLTLAPEGGQHQSVNTPLIGMAADRLASFEPAHVDELGILLRHAFDVMQRDDGSSVW
                     LRLSTRALDQPSRTLDADAVIAGAHWVMPPAHGAHIALAYQGPVAPEALAAFAELRAE
                     EPGAGLLAITSPDRLYAGWLAAQRQRRSGTRNALSHVERVLSVLAPDAALVTVLDGHP
                     ATHAWLGSVRGQRVVALGPDRFGQSGDIPDLYREYGL"
     gene            2470..5184
                     /locus_tag="VFN42_14955"
     CDS             2470..5184
                     /locus_tag="VFN42_14955"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013635007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET8997966.1"
                     /translation="MSDSVIPARLGLRLTPRGHLLAEASADAPELDARVAARLSRASA
                     RGSGYLLLQLGAGEVGQALPAAFTWWRGFAERFVAALCLHASGSEAGSLPRLPAPDAG
                     ERASLALTAPMMPGAEYLTPDMLADLWDATASACADSYAEAGTDLQGFLKALNPAWNL
                     VGRVHFNLAENRRDTEAPFAFMATYTTRLSAQAKAQHVPLGQALREYAGAANRDKLLS
                     LLLPVQRAAERCAWLKPMIEGGEIFHPLRWTPQDASRFLASVPELESAGVVVRMPASW
                     RASRPARPQVSGTVGARKPSGVGLEGLLDFRMEVTLDGEPLSEQEIRALLAGTDSLVL
                     LRGQWVEVDRERLERAMRQFQDAERLAAQNGLSFAEAMRMLSGAAVTDDATEAVSPDW
                     AQVRAGPWLAETLRALRAPDGGGADPGPVLRGTLRPYQRAGVQWLHLLSGLGLGACLA
                     DDMGLGKTIQVLSLLLLERRRNQGPGGMPSLLVAPASLLANWSAEIEKFVPELKARIV
                     HPSAMSAAAVKALSAESLAGLDLAITSYGSLLRIPALAEMQWRFAILDEAQAIKNAGA
                     KQTRAAKALKAQARIALTGTPVENHLGDLWSIFDFINPRLLGSAQQFSRYVKHLAERP
                     ENPYGPLRELVRPYILRRMKTDKAIIADLPDKTEVRAHCSLTKRQAALYEQAVRDLEK
                     ALEENEGIARKGIVLAMLMRLKQICNHPSQWLNDNGWAEQDSGKFARLREIAEVVAAR
                     QQKMLVFTQFREMTAPLATFLNGVFGRPGLVLHGDTAVRNRRELVQRFQDDDGPPFFV
                     LSLKAGGSGLTLTAASHVVHFDRWWNPAVENQATDRAFRIGQKQNVLVHKFVCGGTVE
                     DTVDRLIESKRALSDELLAGGGEINLTELKDDELLQLVALDLNAAMRD"
BASE COUNT          867 a         1740 c         1834 g          854 t
ORIGIN      
        1 gagcccgtat tcgcgataga gatccgggat atcgccgctc tgcccgaacc ggtctggtcc
       61 cagcgcgacc acgcgttgcc cacgtaccga ccccagccag gcatgcgtcg ccggatgccc
      121 gtccagcacg gtcaccagcg ccgcatcggg cgccagcacg ctcagcaccc gctccacatg
      181 cgacagcgca ttgcgcgtgc cggaccggcg ctgccgctgc gccgccagcc agccggcgta
      241 cagccggtcc ggcgaggtga tcgccagcag accggcgccc ggttcttccg cgcgcaattc
      301 cgcgaaggcc gccaaagctt ccggggcgac cggtccctga tacgccaggg cgatgtgggc
      361 gccgtgtgct ggcggcatca cccaatgtgc gcccgcgatc accgcatcag cgtccagcgt
      421 gcgcgacggc tggtccagcg cccgggtgga caggcgcagc cacacggagc tgccatcgtc
      481 gcgctgcatc acgtcgaaag cgtgccgcag caggattccc agctcatcca cgtgcgccgg
      541 ttcgaaggag gccagccggt cggccgccat accgatcagc ggcgtgttca ccgattgatg
      601 ctggccgccc tccggcgcca gcgtcagccc cgatggcgtg gacaccagca ggaaccgcgc
      661 atcctggtaa caggcataaa tcagcgcatc gagcccgcga ttgacgaatg gatcgtacac
      721 cgtgccgatc ggcagcagcc gcgcgcccgt catgctcggc gccagcccgg ccgcgccgag
      781 cagcaggaac aggttctgtt cggcgatgcc cagctcgaca tgctggcctt tgggcgacat
      841 gccccagcgt tgcgcgctgg ccagcttggc gtcgcggaac acgtcattgc gggtatgccg
      901 gtcgaagata ccccgccgat tcacccaggg gccgagattg gtggaaaccg tgacatccgg
      961 cgaggttgtc atgatgtggt cggcaagcgc cttcagctcg ccttggccgc ggccgatctc
     1021 ggccaggatc tcgccgaaac cggactgcgt cgacaccttc cgccctgcca ctttcggcaa
     1081 gggcaaggcg ggcggcaccg gcaccaccgg cgcgctcagc cgccgtccgg cgggagtcag
     1141 tttcgtggca aaagggcagg agtcgagaaa ggcctgcaaa tcctcgggcg cgaggtccag
     1201 cccctcgaag cggtcccact catgccccgg gcggatgttc atctgcgccc ggaagccctc
     1261 catctgctcc ttggtcatca gtccggcatg gttgtcctta tgcccggcga agggcaggcc
     1321 catgcccttg atggtgtagc cgatgaagca ggtaggctgg tccccatccg tctccgcggc
     1381 gcgcaaggcg tcgatgacgg tttcaatgtc gtgcccgccg agattggtca tcagcgcgcc
     1441 cagcgcttcg tcgtccagcg catcgacaat cttgcgtgcc ttgctgcggc ccagatccgc
     1501 cagcaatgcc tggcgccacg ccgcgccgcc ctggaaggtc agcgccgagt acagcgagtt
     1561 ggggcagtcg tcgatccagc ggcgcagtgc ctcgccaccg tcctgcgcga aagccgcctg
     1621 cagcttgcgg ccgtatttga tcgtgacgca gttccagccc atgtcccgga acaggccctc
     1681 gatgcggccg aacagccggt ccggcaccac gctgtccagg ctctggcggt tgtaatcgac
     1741 gatccaccac aggttgcgga cgtcgtgctt ccagccttcc agcagcgcct cgtagatatt
     1801 gccctcatcc agttccgcgt cgccggcgat agcgatatgg cgcccgggcg gtagttcggc
     1861 accggccaac ccgtgcagcc gcacataatc cgccatcagc gccgagaagg tggtcatcgc
     1921 cacgcccagc ccgacggatc cggtggaaaa atccacctcc ggcccatcct tcacccggct
     1981 gggataagac tggatgccgt gcagttggcg caatgtctcc agcttctccc ggctctgccg
     2041 gccgaacaga taattgatgg cgtggaacac cgggccggca tgcggcttca cggcgatgcg
     2101 gtcctgtggg cgcgccacct cgaaatacag cgccgtcatg atggagatga ccgaggcaca
     2161 ggatgcctgg tgtccgccca ctttcagccc atcgcggttc ggccggacgt gattggcgtg
     2221 gtggatcatc caggacgaca gccacagcag cttgcgctcc aactggcgga ggatctgcac
     2281 gcggtgcgca tcggggatgg gtggcgcgag tgtgccgagc gacaggtcca tgcttcggtt
     2341 cccttcgggc gtgggaggcg acgcgggcag gatacagggg agcgggccgg tgtgcacgat
     2401 gggccccggt tcgggcggag agcgcaggtg cgggtggccc gcgggacgcg tcgcagcgat
     2461 actgccggca tgtccgattc ggtgattcct gcgcgactgg gcttgcggct gacgccgcgt
     2521 ggccatctgc tggccgaagc gtccgcggat gcgccggaat tggacgcgcg cgtcgcggcc
     2581 cggttgagcc gggcctctgc ccgcggcagc gggtacctgt tgttgcagct cggcgccggg
     2641 gaggttgggc aggcactgcc tgccgcgttc acgtggtggc gcggctttgc cgaacgtttc
     2701 gtcgccgcgc tgtgcctgca tgcatccggc agcgaagccg gctcgttgcc gcggctaccc
     2761 gccccggatg ccggggagcg cgcgtcattg gcgctgaccg cgccgatgat gccgggtgcg
     2821 gaatacctga cgccggacat gctggccgac ctgtgggatg cgaccgcgtc cgcctgcgcg
     2881 gattcctacg ccgaagccgg taccgacctg cagggcttcc tcaaggcgct gaacccggca
     2941 tggaacctgg tcggccgggt acatttcaac ctggcggaaa accgccgcga caccgaggcg
     3001 ccctttgcct tcatggcaac ctacaccacc cgcctttccg cccaggcgaa agcgcagcac
     3061 gtcccgctcg ggcaggcgct gcgcgaatac gccggtgcag cgaaccgcga caagctgctg
     3121 tccctgctcc tgccggtgca gcgcgcggcg gagcgatgcg cgtggctgaa accgatgatc
     3181 gaaggtggcg aaatcttcca tccgctgcgg tggactccgc aggacgcctc acggttcctc
     3241 gccagcgtgc cggagctgga gagcgccggc gtcgtggtgc gcatgccggc gtcctggcgc
     3301 gccagccggc ccgcgcgtcc gcaggtctcc ggcactgtcg gggcgcggaa gccgtccggc
     3361 gtcgggcttg aggggctgct ggatttccgc atggaggtca cgctggacgg cgagccgctc
     3421 agcgaacagg agatccgcgc gctgctggcc ggcaccgact cgttggtgct gctgcgcggg
     3481 caatgggttg aagtggaccg tgagcggctg gaacgcgcca tgcggcaatt ccaggacgcc
     3541 gaacggctgg cggcgcagaa cgggctgagc ttcgccgagg cgatgcgcat gctgtccggc
     3601 gccgcggtaa ccgatgacgc tacggaggcg gtcagtcccg actgggcaca ggtgcgtgcg
     3661 ggtccctggc tggcggaaac gttgcgggca ttgcgcgcgc cggacggcgg gggggctgat
     3721 cctggcccgg tgctgcgcgg caccttgcgg ccgtaccagc gcgccggggt gcaatggctg
     3781 catctgctat ccgggctggg gctgggtgct tgcctggcgg acgacatggg gctgggcaag
     3841 accatccagg tgttgtcgct cctgctgctg gagcggcggc gcaatcaggg gcctggcggc
     3901 atgcccagcc tgctggtggc tccggcgtcg ttgttggcca actggtcggc ggaaatcgag
     3961 aaattcgtac cggaactgaa ggcacggatc gtgcacccat ccgccatgag tgcggcggcg
     4021 gtgaaggcgc tgtctgccga atcactggcc gggctggacc tggcgatcac cagctatggc
     4081 tcgctgctgc gcattccggc gctggccgag atgcaatggc gcttcgcgat cctggacgaa
     4141 gcgcaggcga tcaagaatgc cggtgccaaa cagacccgcg cggccaaggc actgaaagcg
     4201 caggcgcgca tcgcgctgac tggcacgccg gtggagaacc atctgggcga cttgtggtcg
     4261 attttcgatt tcatcaatcc ccgcctgctg ggttcggccc agcagttttc ccgctacgtg
     4321 aagcacctgg cggagcggcc ggagaatccg tacggcccgc tgcgcgaact ggtgcggcca
     4381 tacattctgc ggcgtatgaa aaccgacaag gcgatcatcg ccgacctgcc ggataagacc
     4441 gaggtgcggg cgcattgcag cctgaccaaa cgccaggccg cgctgtacga gcaggccgtg
     4501 cgcgacctcg agaaagcctt ggaggaaaac gaggggatcg cccgcaaggg catcgtgctg
     4561 gcgatgctga tgcggctcaa gcagatctgc aaccatccgt cacagtggct gaacgacaat
     4621 gggtgggcgg agcaggacag cggcaaattc gcccggctgc gggagattgc cgaggtggtg
     4681 gcggcgcggc agcagaaaat gctggtgttc acccagttcc gggaaatgac agcgccactt
     4741 gcgactttcc tgaacggtgt gttcggccgg cctgggctgg tgctgcacgg cgacaccgcg
     4801 gtgcgcaacc gccgcgaact ggtgcagcgg ttccaggacg acgacggtcc gccgttcttc
     4861 gtgctgtcgc tgaaggccgg cggctccggg ctcacgctga ccgctgcgtc ccacgtggtg
     4921 catttcgacc gctggtggaa cccggcggtg gagaaccagg cgaccgatcg cgccttccgt
     4981 attggacaaa agcagaacgt gctggtgcac aaatttgtct gcggcggcac ggtggaagac
     5041 accgtagacc gcttgatcga atccaagcgc gcgttgtctg atgagctgct ggctggcggc
     5101 ggcgagatca acctcacgga gctgaaggac gacgaactgc tgcaactggt cgcgctggac
     5161 ctgaacgcgg ctatgaggga ctgaacgcca tgtcatatcg gagcgcgagc cgcgggcggt
     5221 attacgagca gcgatggccg gaatacgtcc ctgtcgccga acggcggcgc cgcgccgaac
     5281 tggcaatgga gaagc
//
LOCUS       DASSZH010000693         5658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_323485, whole genome shotgun sequence.
ACCESSION   DASSZH010000693 DASSZH010000000
VERSION     DASSZH010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323485"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VFN42_14960"
     CDS             <1..557
                     /locus_tag="VFN42_14960"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET8997967.1"
                     /translation="IVTALSVPLLRESVGWRRWSAVMVGFAGALIVIRPGGGLHDPSV
                     LLLLGSSLAYALYQIATRSVGNADSAATGIIFTALFGSLAMSLAMPFVFVMPRTLLDT
                     ALFCSLGLLGGSGHYLVIRAFQLGRAAVISPLGYVELIGTTILGYLIFDNFPDAWTWV
                     GATLIILSGLYIALRERRRKERDA"
     gene            637..1605
                     /locus_tag="VFN42_14965"
     CDS             637..1605
                     /locus_tag="VFN42_14965"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET8997968.1"
                     /translation="MSLPIPLTLEPMEAEPIDALPEAAGWSFEPKYDGFRCVVFRDGD
                     SVLLQSRRQRPLARFFPEVAAAVAALAPRRFVLDGELVIRGAGFDALQLRLHPAASRI
                     ARLSRETPATFVAFDMLADTGGDSLLARPFAERRAALQEFPAGDGIAIAESTRSRDTA
                     LRWLKDVGHGLDGIVAKRLDLPYRPGERAMLKYKLWHTIDCVVGGVYRKAGSEDVEYL
                     LLGLYDSEGRLNYVGRCPAHPHEAAYARKLRPLAGSGGFTGNQPGGRSRWSKQERIAT
                     PLRPEQVVEVSVDTITAGKFRHGSRLLRWRTDKAPHQCTMDQIARR"
     gene            1728..3254
                     /locus_tag="VFN42_14970"
     CDS             1728..3254
                     /locus_tag="VFN42_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HET8997969.1"
                     /translation="MNVLTRQNTMARANTFAFAGIEAVPVEVQVQISSGMPAFLVVGL
                     PDKAVGEARERVRAALSAMSLSLPPKRVLVNLKPADLLKEGSHFDLPIALGVLAAMDV
                     LPRDEIAGYAGLGELSLDGTLNKVAGVLPAAIAASAQELGLICPAGQGGEAAWAGRIE
                     VLAPNDLLALINHFRGTQVLTPPDSAGVAEAPRGPDLAEVKGMETAKRALEIAAAGGH
                     NLLLVGPPGSGKSMLAARLPGLLPDLEPREALEVSMIHSVAGLLEGGRLVKRPPFREP
                     HHSASQPALTGGGQRARPGEVSLAHRGVLFLDELPEFPRMALESLRQPMESGRTTVSR
                     ATAHITYPARFQLVAAMNPCRCGFLGEAGRECGRAPRCGEDYQGRISGPLLDRIDLTM
                     EVEPASLSELARAPAGEPSAAVANRVLQARQAQRARYGADGPFNNAEADPRTVRLAPE
                     AHRLIEQAMDRLRLSPRGYTRVLRVARSIADLAGAELVGRVHAAEALAFRHRGIGRGG
                     "
     gene            complement(3410..5218)
                     /locus_tag="VFN42_14975"
     CDS             complement(3410..5218)
                     /locus_tag="VFN42_14975"
                     /inference="COORDINATES: protein motif:HMM:NF039722.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6311 domain-containing protein"
                     /protein_id="HET8997970.1"
                     /translation="MADTLAAGRSEAAGLTEFLLLARPLPVLVLVSVAGAVVFCLSVF
                     DIGFLLGTSRYWQSPRGLVGNSWADLSTALSGYRYFVRDVWTFPLFQTTKLGLPHAVN
                     VVSTSIPLVCLFGRLLYQATGLTVNPYGAWAALCFVGSAVSMTALVAQLGGRGLAAAL
                     AATVFGLCMPALLARWGHLSLMAQWEPILALLAYARAGTGPWRMCGIGLSLLLTAVVL
                     CTDAYLFAMVMGILSATLVRGLAERRLSLRRAGMVVVLFAGVVGFVLWIGGFLATGGA
                     LQDSGFGILSANLLSPLLPASSAIFPGLNPDVFNPTGGQYEGFGYLGLGGVLLLVAGF
                     PWVCAAVYRKCNRLAPLLAVLTVFALFAFSNQIYAGPWHVVHVPLPAPLLTLASIFRS
                     SGRFIWPCLYALTAALIVGVVARWGRIGVWLLVAAALLQIMDTASLRSALSVRTAEGA
                     ILPLHQADWTTAIGQHGFLRVIPAYGCARENGVRQQMAVELELLGSRSGIATNNVYAA
                     RHHYACGGPPVTALAGDELLVFLLPTSQAKVSAALAAHCAASTRVMVCSRKLDHAQLA
                     ALTRVRPVGCSSILSYEDMGFRLYDKCVDAPERAKN"
     gene            complement(5269..>5658)
                     /locus_tag="VFN42_14980"
     CDS             complement(5269..>5658)
                     /locus_tag="VFN42_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997971.1"
                     /translation="YDHLPAFAVMDRAAFFPYLFTNWMPVEVAPRNRLLSDGGVGPIS
                     PSVLIASLTESTADDPALAAEARRHFGHAYWVDWWRRFDYVLWIDFGRHFPPHIWHLS
                     PVAGGSFFEIYRVERPEAERGSGDAQG"
BASE COUNT          971 a         1935 c         1946 g          806 t
ORIGIN      
        1 tgatcgtcac cgccttgtcg gtgccgctgc tgcgggagag tgtcggctgg cgccgctgga
       61 gcgcggtgat ggtcggcttt gccggcgcgt tgatcgtgat ccgcccgggc ggcgggctgc
      121 acgacccctc cgtgctgctg ctgctgggtt catccctcgc ctatgcgctg taccagatcg
      181 ccacccgctc ggtcggcaat gccgacagtg ccgccaccgg catcatcttc accgcgctgt
      241 tcggctcgct ggcgatgagc ctggcgatgc ccttcgtctt cgtcatgccc cgcaccctgc
      301 tcgacacggc actgttctgc agcctcggcc tgctcggggg ctccggccac tacctggtga
      361 tccgcgcctt ccagctcggc cgcgccgcgg tgatttcgcc attgggctat gtcgagctga
      421 tcggtacgac cattctgggc tacctcatct tcgacaattt cccggatgcc tggacctggg
      481 tcggcgccac gctgatcatc ctgtccggcc tttacatcgc cctgcgcgaa cgccggagga
      541 aggagcgcga cgcctgatcc tggcaacgcg gccgtcccgg cggcgtttcg cctgccgccg
      601 cagccacgca gggcacgggc ggcgatgcga ggcggcatgt ccctcccaat tccgctgaca
      661 ctggagccga tggaagccga accgatcgat gcgctgccgg aggcggcggg ctggagcttc
      721 gagccgaaat acgacggctt ccgctgcgtc gtgttccgcg acggcgacag cgtgctgttg
      781 cagtcgcgcc ggcagcgccc cttggcccgg ttcttccccg aggtggccgc agcagtcgcg
      841 gcgctcgcac cgcggcgctt cgtgctggac ggcgagctgg tgatccgcgg cgccgggttc
      901 gacgcgttgc agctccggtt gcacccggcg gcgtcgcgca tcgcacgcct gtcgcgcgag
      961 acgccggcga ccttcgtcgc cttcgacatg ctggcggata ccggcggcga ctcgctgctg
     1021 gcacggccct tcgccgaacg gcgtgccgcg ttgcaggaat ttcccgccgg tgatggaatc
     1081 gcgatcgcgg agtccacgcg gtcgcgcgac acggcgctgc gctggctgaa ggatgtcggc
     1141 cacgggctgg atggcatcgt cgccaagcgg ctggacctgc cgtaccggcc gggcgaacgc
     1201 gccatgctga aatacaagct gtggcacacg atcgattgcg tggtcggcgg ggtttaccgc
     1261 aaggccggca gcgaggacgt cgaatacctg ctgctgggcc tgtacgattc ggagggccgg
     1321 ctgaactatg tcgggcgttg cccggcccac ccgcatgagg ccgcatatgc gcgcaagttg
     1381 cgcccgctgg ccggcagcgg cggtttcacc ggcaaccagc ccggcggccg cagccgctgg
     1441 tcgaagcagg agcggatcgc cacgccgctg cgcccggaac aggtggtgga ggtcagcgtg
     1501 gacaccatca ccgccgggaa gttccgccac ggctcccgcc tgctgcgctg gcgcaccgac
     1561 aaggcgccgc accagtgcac gatggatcag atcgcgcgcc gctgagcttc gcggcggtgc
     1621 cgactccgtc gcggcgggaa ggcccgccac gacgttgatg acgggattag cgttgccttc
     1681 accttttccc gccagagtgc cggcgttgca gctccagcgg cccgacgatg aacgtgctga
     1741 cccggcagaa cacgatggcc cgcgccaaca ccttcgcctt tgccggcatc gaggcggtgc
     1801 cggtggaggt gcaggtgcag atttccagcg gcatgccggc cttcctggtc gtcggactgc
     1861 ccgataaggc ggtgggtgag gcgcgcgagc gggtgcgcgc cgccttatcg gccatgagcc
     1921 tgtcgctgcc acccaagcgc gtgctggtga atctgaaacc ggccgatctg ctgaaggaag
     1981 gcagccattt cgacctgccg atcgcgctgg gcgtgctggc cgccatggac gtgctgccgc
     2041 gcgacgaaat cgccggctat gccggtctgg gcgaactgtc cctggatggc acactgaaca
     2101 aggtggccgg cgtgctgccg gcggcgattg ccgcctcggc gcaggaactc ggcctgatct
     2161 gccccgccgg ccagggcggc gaggcagcct gggccggacg gatcgaggtg ttggcgccga
     2221 acgacttgct ggcgctgatc aaccatttcc gcggcaccca ggtgctgacc ccgccggata
     2281 gcgccggcgt ggcggaggcg ccgcgggggc cggaccttgc cgaagtgaag gggatggaga
     2341 ccgccaagcg cgcgctggag atcgccgcgg ccggcggcca caacctgctg ctggtcgggc
     2401 cgcccggctc gggcaaatcg atgctggccg cgcggctgcc tggactgctg cccgacctgg
     2461 aaccgcggga ggcgctggag gtcagcatga tccacagcgt cgccggcctg ctggagggcg
     2521 gacggctggt gaagcggccg ccgttccgcg agccgcatca ctcggcctcg cagcccgcgc
     2581 tgaccggcgg cggccagcgt gcccggccgg gcgaggtcag cctggcgcat cgcggcgtgc
     2641 tgttcctcga cgaactgccg gaattcccac gcatggcgct ggaatcgctg cgccagccga
     2701 tggagtcggg ccgcaccacg gtttcccgcg cgaccgccca catcacctat ccggcgcggt
     2761 tccaactggt ggcggcgatg aatccgtgcc gctgcggctt cctgggcgaa gccggccgtg
     2821 aatgcgggcg ggcgccgcgc tgcggggaag actatcaggg ccgcatcagc ggcccgctgc
     2881 tggaccggat cgacctgacc atggaggtgg aaccggcctc actctccgaa ctggcccgtg
     2941 cccccgcggg cgagccgagc gccgccgtgg caaaccgggt gctgcaggct cggcaggcac
     3001 agcgggcgcg ctacggggcg gatggaccgt tcaacaatgc tgaagcagac ccgcgcacgg
     3061 tgcggctggc gccggaggcg caccggctga tcgaacaggc gatggaccgg ctgcgcctgt
     3121 cgccgcgcgg ctacacccgc gtgctgcgcg tggcacgcag cattgcggac cttgccggcg
     3181 cggagcttgt cgggcgggtg catgcggcgg aggcgctggc gttccggcat cgcgggatcg
     3241 ggcggggcgg ctgaaagccg cgtcataccg cccgcgctga cgccgaccct ccggtgtcat
     3301 ggccggcgca ggccggctac ccacgccttc tctcgggcgc acacggagac gtgggcgccc
     3361 gcgccaagcg cgggcatgac acagggggca agggttacgc ggcttggcat cagttcttcg
     3421 cacgctccgg cgcgtcgacg catttgtcgt acagccggaa tcccatgtct tcataggaca
     3481 ggatcgagct gcaccccacc ggccggaccc gcgtgagcgc cgccagctgc gcatgatcca
     3541 gcttccggct gcacaccatg accctggtgg acgcggcgca gtgggccgct agcgcggcgg
     3601 acaccttggc ctgcgaggtc ggcagcagaa agaccagcaa ttcgtcgccg gccagtgccg
     3661 tcaccggtgg tccgccgcag gcatagtgat ggcgggccgc gtagacgttg ttggtggcaa
     3721 tgccggagcg agatcccagc aactccagct caaccgccat ctgctggcgg actccgttct
     3781 cccgcgcgca accataggcc ggaatgacgc gcaggaagcc gtgctggccg atggcggtgg
     3841 tccagtccgc ctgatgcagc ggcaggattg ccccctctgc cgtgcgcacc gacagcgcgg
     3901 accgcaggga cgcggtgtcc atgatctgca gcagggccgc ggccaccagc agccagacgc
     3961 cgatcctgcc ccagcgggca acgactccca cgatcagcgc ggcggtaagc gcgtacaggc
     4021 atggccagat gaaccggccg ctcgagcgga agatgctggc gagcgtgagc agcggtgcgg
     4081 gcagcggaac atgcaccacg tgccacggtc cagcgtaaat ctggttggaa aatgcaaaca
     4141 gagcgaagac ggtgagaacc gccagcaagg gtgcgagccg gttgcacttc cggtacacgg
     4201 ccgcgcagac ccagggaaac cccgcgacaa gaagcaggac tcctcctagt ccgagatagc
     4261 cgaacccctc gtactggccg ccggtgggat tgaagacatc cggattcagt ccggggaaaa
     4321 tcgcgcttga tgccgggagc agcggggaca acagattcgc cgacaggatg ccgaagccgc
     4381 tgtcctgcaa cgcgccaccc gtcgcgagaa agccgccgat ccagagcacg aaccccacga
     4441 cgccggcaaa cagcaccaca accatgccag cccggcggag cgacagccgg cgctcggcca
     4501 gcccgcgcac cagcgttgcc gagagtatcc ccatgaccat ggcgaacagg taggcatcgg
     4561 tgcaaagcac gactgccgtc agcagcaggg acaagccgat gccacacatg cgccagggac
     4621 cggtgccggc gcgagcgtac gcgagcagcg caagaatcgg ctcccattgg gccatcaggg
     4681 acagatggcc ccagcgtgcg agcaacgccg gcatgcacag gccgaagacc gttgccgcca
     4741 gagccgcggc gagcccgcgt ccgccaagct gcgcaacaag cgcggtcatc gacacggccg
     4801 acccaacaaa acacagcgca gcccaggcac catatgggtt cacggtcagg ccggtggctt
     4861 ggtacagcag ccggccgaac aggcacacga ggggaatgct ggttgagacg acgttgacgg
     4921 cgtggggaag cccaagcttc gtggtttgaa acagcgggaa ggtccaaaca tcacgaacga
     4981 aataccggta cccgctcagc gcggtagaca gatcggccca gctgttgccg accagcccgc
     5041 gaggggactg ccaataccgc gaggtaccaa gcagaaagcc gatatcgaac accgacaggc
     5101 agaacacgac cgccccggca acactcacca gcacgagcac cggcagcgga cgggcaagca
     5161 gaagaaactc cgtcaaaccc gcggcttccg atctgcccgc tgccagggtg tcagccatgg
     5221 acagacttgc aggcgcgctc ccggacatgg cggaccgggc ccacgctctc atccctgcgc
     5281 atcacccgag cccctttccg cttctggccg ctcgacacgg tagatctcga aaaaggatcc
     5341 acccgcgacg ggagacagat gccagatgtg cggcggaaag tgccggccga aatcgatcca
     5401 cagcacgtaa tcgaagcgcc gccaccaatc gacccaatat gcatgtccga aatgccggcg
     5461 cgcttctgcc gcaagagccg ggtcatcagc cgtagactcg gtgagcgaag cgatcagcac
     5521 actcggcgaa atcggtccga caccaccatc ggacagcagc cgattgcgcg gcgccacctc
     5581 caccggcatc cagttggtga acaggtaggg aaagaacgcc gcccggtcca tcacggcgaa
     5641 ggcgggaaga tggtcgta
//
LOCUS       DASSZH010000694         2097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_323577, whole genome shotgun sequence.
ACCESSION   DASSZH010000694 DASSZH010000000
VERSION     DASSZH010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323577"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFN42_14985"
     CDS             <1..205
                     /locus_tag="VFN42_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HET8997972.1"
                     /translation="VEEANVHGTVASVGVRFVSDQVSYTRDKAGHTVLGTEAVTEITD
                     VWTFERDLSSRDPTWRLVAARSA"
     gene            complement(246..1208)
                     /locus_tag="VFN42_14990"
     CDS             complement(246..1208)
                     /locus_tag="VFN42_14990"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET8997973.1"
                     /translation="MPSASRTTSKSRPKAKTAAPARTAAPAAEATAPDYSIAAVERAL
                     DLLEALARVGPAPLATLAEAAGCSRTAAFRLLRTLQGRGFAIQDESRGIWRLGARWGV
                     LGRAAVEQGALAATAMPFLTELGKLTGENVYLRVRDGMENETIAIFQSDPALRVYTET
                     GKRGPLHAGSARMLLAHAPEAVQTQVLAQRLPRFTPATRTDSAWIAADLQRIRTRGYL
                     ITSDEVVPGAVSVTAPVRDASGQVIAALSLGAPSMRMRPPRPRTLLPQVMEAAARLSQ
                     ALGDRNPAPAKPANGQNGASAAAAHRWPGNGHGAASPGPHSMFR"
     gene            complement(1467..>2097)
                     /locus_tag="VFN42_14995"
     CDS             complement(1467..>2097)
                     /locus_tag="VFN42_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997974.1"
                     /translation="FPDYLSYHFGNRHAPDGTLNPRAQWTYGVVPRLFAALREQGVTR
                     QERYSLFGHSAGGQFVHRMLSFGYRDRVAVAISANAGTYSMPDLETPWPFGLGATGLD
                     ADALRDLLGFPLIVMAGTADTQTTGPYFPGGPNAMRQGAHRHARAHNYVRAGQDAARR
                     LGVACAWRVIDVPGVGHDGAGMSAAAAPVVAQALQAADGSGNRPGAVAP"
BASE COUNT          333 a          708 c          745 g          311 t
ORIGIN      
        1 tgtcgaggaa gcgaacgtgc acggtacggt ggcatcggta ggcgtgcgct tcgtgtccga
       61 ccaggtcagc tacacccgcg acaaggctgg ccacaccgtg ctcggcaccg aagcggtcac
      121 cgagatcact gacgtgtgga cgttcgagcg cgatctctcc agccgcgacc cgacctggcg
      181 cctggtcgcc gccagaagcg cctgatctca gcagcgcctc tccgccccgc gggcggggag
      241 gctgttcagc ggaacatcga atgcgggccg ggcgaggcgg cgccgtggcc attgccgggc
      301 cagcgatgcg ccgcagcggc ggatgcgcca ttctggccgt tggccggctt ggccggggcc
      361 gggttgcggt cgcccagcgc ctgcgacagc cgcgctgccg cttccatcac ctgcggcagc
      421 agagtccgtg gacgcggcgg ccgcatgcgc atcgacgggg cgccaagcga caaggccgcg
      481 atcacctggc cggaggcatc gcgcaccggt gcggtcacgc tgacggcgcc cggaaccacc
      541 tcatcggacg taatcagata gccgcgggtg cggatccgct gcagatcggc ggcaatccag
      601 gcagagtcgg tgcgggttgc cggggtgaag cgcggcagtc gctgtgccag cacctgggtc
      661 tgcaccgcct ccggcgcatg cgccagcagc atgcgggcgc tgccagcgtg cagcggaccg
      721 cgcttgccgg tctccgtata gacccgcaag gctggatcgc tctggaagat tgcgatggtc
      781 tcgttctcca tcccgtcgcg cacccggaga tagacgttct ccccggtcag cttgcccaat
      841 tcggtcagga acggcattgc ggtcgcggcc agcgccccct gttccacggc ggcccgtccc
      901 agcacgcccc agcgggcacc caggcgccag atgccgcggg attcatcctg gatagcgaag
      961 ccgcggccct gcagggttcg caacaggcgg aaagcggcgg tgcggctaca cccggcagcc
     1021 tcggccagcg tcgccagcgg ggccggaccg acccgggcca gcgcttccag caggtcgagg
     1081 gcacgctcta ccgcggcgat gctgtagtcg ggggcggtgg cctccgccgc cggagcggcg
     1141 gtccgggcgg gggcggccgt cttggctttc ggacggctct tgctggttgt gcggctagcg
     1201 gacggcatga cggaatcctt ggcacggagc agcgcggctc ggcaggcgca gggagttcgg
     1261 ttcgatcgcg tcagataccc aggctgtgga aggggtggat aacatggcac ccggggttgc
     1321 gttcgatcaa agccagtacc ggatattacc gatcagtagg ccctgcaaga tacaaattca
     1381 cgcacctccc gtttgccgaa cggcaaattt atggaattcc agcgtgataa tgtcgctaca
     1441 cttccttatg gataaggcac cgctggtcag ggggcaaccg cgccagggcg gtttccactc
     1501 ccgtccgcgg cctgcaaagc ctgggcgacg acgggtgccg cggcggccga catgccggca
     1561 ccgtcatgcc ccaccccggg tacgtcgatc acacgccagg cgcacgccac gcccagccgc
     1621 cgcgccgcgt cctgccccgc gcgcacgtaa ttgtgtgcgc gcgcgtggcg atgggcgccc
     1681 tggcgcatgg cattggggcc gccggggaag tacggcccgg tggtctgcgt gtcggcggtc
     1741 cccgccatga cgatcagcgg aaaccccagc agatcgcgca gggcgtcggc atccaggccg
     1801 gttgcgccga gaccgaaggg ccagggggtc tccaggtcgg gcatggaata ggtcccggca
     1861 ttggcgctga tcgccacggc cacgcggtcg cgatagccga aggagagcat gcggtgcacg
     1921 aactgcccgc ccgccgaatg gccgaacagg ctgtagcgtt cctggcgggt gaccccctgc
     1981 tcacgcagcg ccgcgaacag ccgaggcacc acgccatagg tccattgcgc gcgcgggttc
     2041 agtgtgccgt ccggcgcatg cctgttgccg aagtggtagc tcagataatc ggggaaa
//
LOCUS       DASSZH010000695         3501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_698470, whole genome shotgun sequence.
ACCESSION   DASSZH010000695 DASSZH010000000
VERSION     DASSZH010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698470"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="VFN42_15000"
     CDS             <1..942
                     /locus_tag="VFN42_15000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HET8997975.1"
                     /translation="ATYDRMRIVIMGAGALGGYFGARLAAAGNDLAFVARGAHLAAIQ
                     RQGLRITSALGDLHLRDVVATDDPRTLGPADLVLIGVKLWDLEAAAQAVKPLVRPGTA
                     VVAFQNGVGKDDTLVRALGREAVIGGMAQIGVVIAEPGVIAHTGTMAKLTFGELDNSR
                     SARVEAFQAACAAAGIDAEIPPDIRLATWEKFVFLVGMSAVTASMRSRIGPIRANPEA
                     RGLLADLMQEAVAVGQAQGVPLAQDYVERRMAFIDRLPAEMTASMQGDLARGNRLELP
                     WLSGAVVEMGRALGIPTPLNRAVSGVLALYAEGAPRG"
     gene            complement(982..2154)
                     /locus_tag="VFN42_15005"
     CDS             complement(982..2154)
                     /locus_tag="VFN42_15005"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HET8997976.1"
                     /translation="MDDIVIVSAARTPVGSFNGALGSLPAHSLGSIALQAAFERAGIE
                     AGDVDEVILGQVLAAGEGQNPARQAARAAGLPDAKTAFGINQVCGSGLRAVALAAQQI
                     RTGESAIVAAGGMESMSQSQHSAYLRAGTKMGGVEFIDTMLKDGLWDAFHGYHMGNTA
                     ENVATKYQITREQQDQFALASQQKASAAQKAGRFKDEIAPVTIKGRKGDTVVADDEYI
                     RHDASLETMTRLRPAFSKDGTVTAGNASGINDGAAALVLMTASEANRRGLTPLARIAG
                     FATAGVDPAVMGTGPIPATRKVLQRAGWKVDDLDLVEANEAFAAQALAVNKDLGWDTG
                     KVNVNGGAIAIGHPIGASGARILVTLLHEMQKRDAKKGLATLCIGGGMGVAMCLER"
     gene            2334..>3501
                     /locus_tag="VFN42_15010"
     CDS             2334..>3501
                     /locus_tag="VFN42_15010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03087 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HET8997977.1"
                     /translation="MPDLLFLTQRIPYPPHKGEKIRPLQILRHLRRSHDIHLGCLVDD
                     PRDMQHVPTVQSLCTGSYFAPLDRRRAKLTCMTGLLTGEALSVTFYRDRGLAAWVQRV
                     LRDIRPEVIFVCSSNMAPYVLDLRGQERVCLVDLADVDSEKWRAYAQKGSPIMRWVHA
                     REWRRTAALEARIARECDWSTFVSVEEATLFAGMHPEFAERIRAVGSGVDHDYFDPAQ
                     DFFAPYPTDRLNFVFTGTMDYPPNVDAVVWFAETVLPIIRRSAPSAAFHIVGSNPSPA
                     VKALTARDGVSVTGRVPDVRPYLAHCTAGVAPMRIARGIQNKVLEAMAMGRPVVVTGD
                     ALEGIDAAPGTQVLLAETPDGLAAACLLAATSKGAAIGQAARAHIVGNFGWPEKL"
BASE COUNT          502 a         1176 c         1224 g          599 t
ORIGIN      
        1 gccacgtatg atcgcatgcg aatcgtgatc atgggcgctg gcgcgctcgg cggctatttc
       61 ggcgctcgcc tggctgcggc aggcaatgac ctcgccttcg tcgcgcgcgg cgcgcatctg
      121 gcggccatcc aacggcaggg actgcgcatc accagcgcct tgggtgatct gcatctgcgc
      181 gacgtcgtgg cgaccgacga tccgcgcacg cttgggccgg ccgacctggt gctgatcggg
      241 gtgaagctgt gggacctgga agccgccgcg caggcggtga agccgctggt gcggcccggc
      301 accgcggtgg tggccttcca gaacggcgtg ggcaaggacg acacgctggt gcgcgcgctc
      361 ggccgtgagg cggtgatcgg cggcatggcg cagatcggcg tagtgatcgc cgaacccggg
      421 gtgatcgcgc ataccggcac gatggcgaag ctgaccttcg gcgagctgga caacagccgc
      481 tctgcccggg tggaggcatt ccaggctgcc tgcgccgcgg ctgggatcga tgccgagatc
      541 ccgcctgaca tccggctggc gacctgggag aagttcgtct tcctggtggg catgtcggcg
      601 gtgaccgcaa gcatgcgcag ccgcatcggt ccgatccgcg ccaatccgga agcgcgcggt
      661 ttgctcgccg acctgatgca ggaggcggtg gcggtgggac aggcccaggg cgtaccgctg
      721 gcgcaggact acgtcgagcg ccggatggcg ttcatcgacc ggctgccggc cgagatgacc
      781 gcctcgatgc agggtgacct cgcacgcggc aaccggctgg agcttccctg gctcagcggc
      841 gcggtggtgg aaatgggccg cgcactcggc atcccaacgc cgttgaaccg cgcggtcagc
      901 ggcgtgctgg cgctttacgc ggagggtgcg ccgcggggct gacggcgtcg ccgcccgtcc
      961 cgcggcagcc tgcgctgcgc gctagcgctc caggcacatg gcgacgccca tgccgccgcc
     1021 gatgcacagc gtcgccagcc ccttcttcgc gtcgcgcttc tgcatctcgt gcagcagcgt
     1081 gaccaggatg cgggcaccgg atgcgccgat cggatggccg atggcgatgg cgccgccgtt
     1141 cacgttcacc ttgccggtgt cccagccgag gtccttgttc accgccaggg cctgcgcggc
     1201 gaaggcctcg ttggcctcga ccaggtccag atcgtccacc ttccagccgg cgcgctgcag
     1261 caccttgcgc gtggccggga tcgggccggt gcccatcacc gccggatcga caccggcggt
     1321 ggcgaagccg gcgatccgtg ccagcggcgt cagcccgcgc ctgttcgcct ccgaagcggt
     1381 catcagcacc agcgctgccg cgccgtcatt gatcccggag gcattgcccg cggttaccgt
     1441 gccgtccttg ctgaaggccg ggcgcagccg ggtcatggtc tccaggctgg cgtcgtggcg
     1501 gatgtattcg tcgtccgcga cgaccgtatc gcccttgcgg cccttgatgg tgaccggcgc
     1561 gatctcgtcc ttgaagcggc cggctttctg cgcggcgctg gccttctgct gcgatgccag
     1621 ggcgaactgg tcctgctgct cccgggtgat ctggtacttg gttgcaacgt tctccgcggt
     1681 gttgcccatg tggtagccgt ggaaggcgtc ccagagaccg tccttcagca tggtgtcgat
     1741 gaactcgacg ccgcccatct tggtgccggc gcgcagatag gcgctgtgct gcgactggct
     1801 catgctctcc atgccgccgg cggcgacgat ggcgctttcg ccggtgcgga tctgttgcgc
     1861 cgccagtgcg acggcgcgca ggccggagcc gcagacctgg ttgatgccga acgcggtctt
     1921 ggcgtccggc aatccggcgg cgcgggccgc ctggcgggcg ggattctgtc cctcaccggc
     1981 ggcgagcacc tggcccagga tcacttcatc gacgtcgccc gcctcgatgc cggcccgttc
     2041 gaaggcggct tgcagggcaa tgctgcccag gctgtgcgcg gggagggacc cgagggcgcc
     2101 gttgaaactg cccaccgggg tgcgggcggc ggagacgatg acgatgtcgt ccatgtccga
     2161 tctccttgtc agaaggggac agattacccc gggtttcgca ggtgcgaaag gcacggccgt
     2221 gaggctgccg aaatgcttta cccgccccgc cgaaaccgcg taacaggggc gcaacgggtt
     2281 cgccagtaga cagatcgaac ctggcgcatt gtccgttccc gagtggccca gccatgcccg
     2341 acctgctgtt cctgacccag cgcattccct atccgccgca caaaggggag aaaatccggc
     2401 cgctgcaaat cctgcgtcac ctgcggcggt cgcatgacat ccaccttggt tgcctggtgg
     2461 atgacccgcg cgacatgcag cacgttccca ccgtgcaatc gctgtgcacc ggctcctact
     2521 tcgcgccgct cgaccgccgc agagccaaac tgacctgcat gaccggcctg ctgaccggcg
     2581 aggcgctcag cgtcaccttc tatcgcgacc ggggcctcgc agcctgggtg cagcgcgtgc
     2641 tgcgtgacat ccggccggag gtgatcttcg tctgctcgtc caatatggcg ccctacgtgc
     2701 tggacctgcg cggacaggag cgggtctgcc tggtggatct tgcggatgtc gattcggaga
     2761 aatggcgcgc ctacgcgcag aagggatcgc ccatcatgcg ctgggtgcat gcgcgggagt
     2821 ggcgccgcac cgccgcgctg gaagcgcgca tcgcccggga atgcgactgg agcacgttcg
     2881 tttccgtcga ggaggcaacg ctgtttgccg gcatgcaccc ggaatttgcc gagcggatcc
     2941 gcgcggtggg cagtggcgtc gatcatgact atttcgaccc ggcacaggac ttcttcgccc
     3001 cttatcccac cgaccggcta aatttcgtgt tcaccggcac gatggactac ccgcccaatg
     3061 tcgatgcggt ggtgtggttc gccgaaaccg tgttgccgat catccgccgc agcgctccga
     3121 gcgccgcctt ccacattgtc ggatccaatc catccccggc cgtaaaggca ttgaccgcac
     3181 gtgacggcgt gtcggtcacc ggtcgtgtgc ccgatgtgcg cccctatctc gcgcattgca
     3241 ccgccggcgt tgcgccgatg cggatcgccc gcggtatcca gaacaaggtg ctggaggcaa
     3301 tggcgatggg ccggccggtg gtggtcaccg gcgatgcgct ggagggcatc gacgccgcac
     3361 cgggcacgca ggtgctgctg gccgagacgc cggacgggct tgcggccgcc tgcctgctgg
     3421 cggccaccag caagggcgcc gcgatcggtc aagcagcgcg ggcgcatatt gtcggcaatt
     3481 tcggctggcc ggagaaactg g
//
LOCUS       DASSZH010000696         3418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1602162, whole genome shotgun sequence.
ACCESSION   DASSZH010000696 DASSZH010000000
VERSION     DASSZH010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602162"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1155)
                     /locus_tag="VFN42_15015"
     CDS             complement(97..1155)
                     /locus_tag="VFN42_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET8997978.1"
                     /translation="MRIAQISPLFEAVPPKLYGGTERVVYSLTEELVAMGHDVTLFAS
                     GDSVTSATLAPMRERALRLDPSVVDWVSIYHGMVEKIRRRSDEFDVLHFHIDYFPLSL
                     FSRQPTPFLTTLHGRLDIHEFKDIYELYPNAPFVSISDSQRRPIPRINWARTVLHGMP
                     SQLLTPQPVKQDYLAFLGRISPEKGVDKAIRIAGKAGMKLKIAAKVDKADQVYWDEEI
                     KPLVQASPWVEFIGEINDHQKPEFLSGAHALLFPIDWPEPFGLVMIESMACGTPVIAY
                     NRGSVPEVIDHGVSGFIVHDETSAVSALERLGDLDRGTVRATFDRRWTARRMAEDYVD
                     LYEELCEVNVPLLRAVGD"
     gene            1354..3177
                     /locus_tag="VFN42_15020"
     CDS             1354..3177
                     /locus_tag="VFN42_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006557033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8997979.1"
                     /translation="MAIIEKLPRPTVAEMRQNLAVLPGFRGHPFAFLARYVRRHPLGH
                     AVVLASVLIAVGCSVGTQYAMKRLIDIIAQGPQGAGSAMWWAFALVCVLIAADNLLWR
                     VGGYAAARTYVAVTGDIRAELFAYVSGHAPSYFAERLPGALAGRIGAAANAAFTIENT
                     GSWNVLPPSIAVVFSLALIGLVSPSLAATLVGFSLALGGLVFWLARQGAPLHRAYAEK
                     AAAVDGELVDVVGNYHVVRAFGATLREQHRIGRTVDSEMAARRSSLFYMERLRLIHAA
                     LTVLASAGVIAWGLLLWQVGRASVGDILLLTSLAITILHCTRDLAVALVDLTQHVARL
                     EEAIGTLLAPHELPDRADARALLPGLGQVVFNRVSFSYPKRAPVLREFDLVIQPGQRI
                     GLVGASGAGKSTVLALLQRFYDVSGGQILVDGQDISAVTQDTLRQSMAIVPQDISLFH
                     RSVLENIRYAKPGATEAEVLAAAEMAYCRDFIEALPEGFDTMVGDRGVKLSGGQRQRL
                     AIARALLKDAPILLLDEATSALDSESERAIQSALDRLMQGRTVIAIAHRLSTLRSFDR
                     IIVMDAGRIVEDGAPDELAARPGPYRELLLKQIMEPVPQAA"
BASE COUNT          547 a         1121 c         1165 g          585 t
ORIGIN      
        1 cggccttgag cgaggcatgc gtggcgggcc tgcgtccgcc acgacgtaac gtgcctatgt
       61 tggcgcctat gggaacgcac ccgccccgca acgcgatcag tcgccgaccg cgcgcagcag
      121 cggcacgttc acttcgcaca gctcctcgta cagatcgacg tagtcctcgg ccatgcggcg
      181 cgcggtccag cggcgatcga acgtggcacg caccgtgccg cggtccagat cgcccagccg
      241 ctccagcgcc gacaccgccg acgtctcgtc atgcacgatg aagccgctga cgccgtggtc
      301 gatcacctcc ggcaccgagc cgcggttgta ggcgatcacc ggcgtgccgc aggccatgct
      361 ctcgatcatc accaggccga acggctccgg ccagtcgatc gggaacagca gcgcatgcgc
      421 gccggagagg aattccggct tctgatggtc gttgatctcg ccgatgaact cgacccacgg
      481 gctggcctgc accagcggct tgatctcttc gtcccagtag acctggtccg ccttgtccac
      541 cttggcggcg atcttcagct tcatgccggc cttgccggcg atgcggatcg ccttgtccac
      601 gcccttctcg ggcgagatgc ggccaaggaa cgccaggtag tcctgcttca ccggctgcgg
      661 cgtcagcagc tgtgacggca tgccgtgcag cacggtccgt gcccagttga tgcgcggaat
      721 cggccggcgc tgcgagtcgg agatcgagac gaacggcgcg ttcggataca gctcgtagat
      781 atccttgaac tcgtggatat ccagccgccc gtgcagcgtg gtgagaaacg gcgtcggctg
      841 ccggctgaac agcgacagcg ggaaatagtc gatgtggaaa tgcagcacat cgaattcgtc
      901 ggaccggcgg cggatcttct cgaccatgcc gtgatagatg gacacccagt ccaccaccga
      961 cgggtcgagc cgcagggcac gctcgcgcat cggcgcaagc gtggccgaag tgaccgagtc
     1021 accgctggcg aacagggtca cgtcgtgacc catggccacc agttcctcgg tcagcgagta
     1081 caccacgcgc tcggtgccac cgtacagctt gggcggcacc gcctcgaaca gaggcgagat
     1141 ttgagcaata cgcatgaggg cgagccccgc tttcctttta ggggttactt caacagatta
     1201 gcgacaattc ttggcttgac ttcttactct caatgccgct atcaactaaa agttatagtt
     1261 tgggaatgtg gcaaaaactc ggcgcaattc ttgcgctgga aagacaccac agcgggccgg
     1321 agccgcttcg aaatccggaa aggagccgct ttcgtggcca ttattgaaaa actgcctcgt
     1381 ccgactgtgg ccgaaatgag gcagaacctg gcagtcctgc ccggtttccg cggccaccct
     1441 tttgctttcc tggcacgcta tgtgcgccgc catccgctcg gccacgccgt ggtgctcgcc
     1501 tcggtgctga tcgccgtcgg ctgctccgtc ggcacccagt acgccatgaa gcgcctgatc
     1561 gacatcatcg cgcagggccc gcagggcgcc gggtcggcga tgtggtgggc gttcgccctg
     1621 gtatgcgtgc tgatcgccgc cgacaacctg ctgtggcggg ttggcggcta tgccgcggcg
     1681 cgtacttacg tcgccgtcac cggcgatatc cgcgctgagc tgttcgcgta tgtctcgggg
     1741 cacgcaccca gctatttcgc ggaacggctg cccggcgcgc tggcgggacg catcggcgcc
     1801 gccgccaacg ccgccttcac catcgagaac accgggtcgt ggaacgtcct gccgccaagc
     1861 atcgcggtgg tgttttccct ggcgctgatc gggctggtga gcccgtcgct ggcggcaacc
     1921 ctggtgggct tctccctggc gctgggcggc ctggtgttct ggctggctcg gcagggcgcg
     1981 ccgctgcatc gcgcctatgc cgagaaggcc gctgccgtgg acggcgaact ggtggacgtg
     2041 gtgggcaact accacgtggt gcgcgcgttc ggcgccaccc tgcgtgagca gcaccgcatc
     2101 ggccgcaccg ttgacagcga gatggcggcg cggcgcagca gcctgttcta tatggagcgg
     2161 ctgcggctga tccacgccgc actcaccgtc ctggccagcg ccggcgtcat cgcctgggga
     2221 ttgctgttgt ggcaggtcgg ccgcgcctcg gtcggcgaca tcctgctgct caccagcctg
     2281 gcgatcacca tcctgcattg cacgcgcgac ctcgcggtgg cgctggtcga cctgacccag
     2341 cacgtcgctc ggctcgagga ggcgatcggc accctgctgg ccccgcatga gctgccggac
     2401 cgcgcggatg cgcgggcact gttgccgggg ctggggcagg tcgtgttcaa ccgcgtcagc
     2461 ttctcctatc cgaagcgggc accggtgctg cgggaattcg acctggtcat ccaacccggc
     2521 cagcgaattg gcctggtcgg tgcttcaggt gccggcaaat ccaccgtgct ggccctgctg
     2581 cagcggttct acgatgtcag cggcgggcag atcctggtgg acggtcagga catcagcgcg
     2641 gtgacgcagg acacgctgcg gcagagcatg gcgatcgtgc cgcaggacat cagcctgttc
     2701 caccgctcgg tgctggagaa catccgctac gccaagcccg gtgcgaccga ggccgaggtg
     2761 ctggcggcgg ccgagatggc gtattgccgc gacttcatcg aggcgctgcc ggaaggcttc
     2821 gacaccatgg tcggcgatcg cggcgtgaaa ctgtccggcg gacagcggca gcgcctggcg
     2881 atcgcgcggg cgctgctgaa ggacgcgccg atcctgctgc tggacgaagc gaccagcgcg
     2941 ctggacagtg agtccgaacg ggcgatccag tcggcgctgg accggctgat gcaggggcgc
     3001 accgtcatcg ccatcgcgca ccggctgtcc acgctgcgca gcttcgatcg gatcatcgtg
     3061 atggatgccg ggcggatcgt ggaggatggc gcgccggacg aactggcggc ccgtcccggc
     3121 ccataccggg agctgctgct gaagcagatc atggagccgg tgccgcaggc ggcctagtgc
     3181 cgcgatgcgc aacggccgat cggaatagtg gaggggaggc ggcggtgtcg atgccgcccg
     3241 tggcggcgca ggcccatagg cgcgagcgtc ggagcctacc cgtcgtggcg ggcgaaggcc
     3301 cgccacccat gtgtggacgg cccgctgggt gcaagggctc ggagcagaaa aactgcgacg
     3361 ggtacgagtg cggtcatgtg tccggccttg cggtgcggtt tcgatgaccg cggcccag
//
LOCUS       DASSZH010000697         5366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_937478, whole genome shotgun sequence.
ACCESSION   DASSZH010000697 DASSZH010000000
VERSION     DASSZH010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937478"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..553
                     /gene="rsmG"
                     /locus_tag="VFN42_15025"
     CDS             8..553
                     /gene="rsmG"
                     /locus_tag="VFN42_15025"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HET8997980.1"
                     /translation="MRRFAELLLRWNASLNLIGRGELRHLWTRHIADSLQLGRIGALP
                     ARAIDLGSGAGFPGLILAIAYGIHVDLVEQDQRKAAFLREAARVTQAPVTVHAMDIAR
                     VALPPAPLVTARALAPVAKLLEYAQPLLTPDGACLFLKGRDADAELAQAAAGWQMQVE
                     KFSSETDANGVILRISQLRRR"
     gene            649..1518
                     /locus_tag="VFN42_15030"
     CDS             649..1518
                     /locus_tag="VFN42_15030"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET8997981.1"
                     /translation="MNDQVTEDPQTLLDRLERQSVQQETPCGDGSMVWHLWNETGRAD
                     APVLVLFHGGAGSWRHWVRTIPELSQHYRVLAPDLPGLGESAFPPNGDDADAVAEIVA
                     DGIDHVIGAGTGYDVAGFSFGGTMAACVAAIHGPRVRSVTIIGSSGVGPLGSAVQLLK
                     VRQLEGQERVEAHRTNLNRLMIADPHKIDALALAIQEWNTVRSRLRTPALSRSGAILR
                     ALDRVQAPLNGIWGEFDAPANPRARERVAALQERHPEADIRLIPGAGHWVAYEAADTV
                     NAMLLEMLARTRP"
     gene            1531..4143
                     /locus_tag="VFN42_15035"
     CDS             1531..4143
                     /locus_tag="VFN42_15035"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET8997982.1"
                     /translation="MSDASAIERGPGEMLGNAGPGMLAGLRVIEVADERAEYAGLLLA
                     GLGAEVIKLEPPEGNATRRIGPFLDDQPGPDRSLFFWHYNRNKKSVVLDWRDPAARDR
                     MLQLLDGADVLLDSSCGALNQVLGLDRAALGARFPRLVTARITPFGDDGPWADFKGSD
                     LIHLALGGVMMNCGYDPDPRLEYDTPPIAPQIWHAYHIAGEQLATGIIAALIHRHCSG
                     EGQDVSIAIHEAVSKNPELDIMHWVMRRVPLWRLTNRHAVETPNHSPSICHTKDGRWF
                     IMHGMGARDLKNLVPLLSKYGMQADLQPPPPDADLKARNVPGSQAGDEARAHMLDVVQ
                     RFVRAWTYADMPWREAQDAGLLWAPLRKPHENALDEHWLKRKSFADVQHPELGRSFRY
                     PTSKWLSNQTSWQVGRRAPLLGEDTQAVLREPTRRPSVPAQPRGVANPVRSALHDKPF
                     PLQGVKILDFAWFLASAGGTRFLAGLGAESYKVEWKDNPDTRLAAMAPVGGRAARDAA
                     TGSLPGVKDHDMGGQFHNKNAGKRGISLNIRHPKGLQIAKDLVRICDVVAEGFSPGVL
                     QRLGLGYDVMKSIRPDIIYIQQAGMGAHGTYGRMRTVGPVAAAFAGQGDMSGLPEPAM
                     PVGWGYSYLDWMGAYGYALALLGALYHRERTGEGQWIDASQCESGLFLTGTTILDWWA
                     NGREWRRYGNRSPYKPAAPHGAYRCAGTDRWIAIACFTDAEWQALAQVAGHTAWLEDR
                     RFATLADRLAHQDALDATVESWTRTQEPHACMLALQRAGVPAGVCQNAEDRCDHDPQL
                     RHLNWLTEVTGSKIGTWPVYELPMKLSHTPAYVGGPINRGAPCYGEDNEWLLTNLLGM
                     SRSDVQRLAEEGVI"
     gene            4343..>5366
                     /gene="phnA"
                     /locus_tag="VFN42_15040"
     CDS             4343..>5366
                     /gene="phnA"
                     /locus_tag="VFN42_15040"
                     /EC_number="3.11.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047400 - phosphonoacetate hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019636 - phosphonoacetate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetate hydrolase"
                     /protein_id="HET8997983.1"
                     /translation="MPGEPAIDHVTVNGRTYAVPQSPVVVVCLDGCDPEYLAAGFAAN
                     ELPVFARIARDGFASESLSALPSFTNPNNCSLVTGVPPSVHGVSGNFYLDRHSGETVM
                     VTGDKELQAPTILAAMAQTGVPTAVVTAKDKLRAALAKGLPIGANGIAFSAQNAAAAR
                     EQTHGIGNVTRLVGRGEPDQYSADLSLFVLDAGVRLLETGRARLLYLSLSDYVQHRYA
                     PQEPEARDFLRAVDARLGRMMDLGAIVAATADHGMHDMAKPDGTPNVMFVGDVLDAAF
                     GPGSCRVICPITDPFVRHHGALGGLVRVYVAAGGPAIRAVTAHLADLPGIDQALEGSE
                     ACRRYDL"
BASE COUNT          897 a         1814 c         1823 g          832 t
ORIGIN      
        1 ccctgccctg cgccgcttcg ccgaactgct gctgcgctgg aatgcctcgt tgaacctgat
       61 cggacgcggc gaactccgcc atctgtggac acgccacatc gccgacagcc tgcagctcgg
      121 ccgcatcggt gcgttgccgg cacgcgcgat cgatctgggt tccggggcag gatttcccgg
      181 gctgatactg gcgatcgcct acggcatcca tgtggatctg gtcgagcagg atcaacgcaa
      241 ggccgccttc ttgcgcgagg cagcgcgcgt cacccaggca ccggtgacag tgcatgcaat
      301 ggatatcgcg cgggttgcgc tgccgcccgc gccgctggtc accgcacgcg cattggcgcc
      361 ggtggcgaaa ctgctggagt acgcccaacc attgctgacc ccggatggcg cctgcctgtt
      421 cctgaagggc cgcgatgccg atgcggaact cgcgcaggcg gcagccgggt ggcagatgca
      481 ggtggagaaa ttcagcagtg aaaccgatgc caacggcgtg atcctgcgga tttcgcaact
      541 ccggcgccgc tgaccacaga ttgccctccg cgtctccgct gtcccggttc gacagcccgc
      601 ccattgcatt cctatgatgc cggcaagcac ccaggggatg gagcaggcat gaacgatcag
      661 gtcaccgagg atccgcagac gctgctggac cggctggaac gtcagtcggt ccagcaggaa
      721 accccgtgcg gtgacggcag catggtctgg cacctgtgga acgaaaccgg ccgcgcggat
      781 gcacccgtgc ttgtgctgtt ccatggcggc gcgggctcct ggcggcactg ggtacgcact
      841 attccggagc tgtcacagca ctatcgcgtg ctggcgcccg acctgccggg gctgggcgaa
      901 tccgcctttc cgccgaacgg tgacgacgcc gacgcggtgg cggaaatcgt agcggacggc
      961 atcgaccacg tgatcggtgc cgggaccggc tatgacgtgg cagggttttc cttcggcggc
     1021 acgatggcgg cctgcgtcgc cgcgatccac ggcccgcgtg tgcgctcggt caccatcatc
     1081 ggctcgtccg gcgtcggtcc gctcggcagt gcggtgcaac tgctgaaggt gcgacaactg
     1141 gaaggccagg aacgcgtcga agcgcaccgc accaacctca accggctgat gatcgccgat
     1201 ccgcacaaga tcgacgcgct ggcgctggcg atccaggagt ggaacaccgt gcgctcgcgc
     1261 ctgcggactc ccgcgctgtc gcgcagcggc gcgatactgc gcgcgctgga ccgcgtgcag
     1321 gcacccctca acggcatctg gggcgagttc gacgcgcccg ccaatccgcg cgcgcgcgaa
     1381 cgcgtcgccg cgctgcagga acgccacccg gaggccgata tccgcctgat ccccggcgcc
     1441 ggccattggg tggcgtacga agcggcggac acggtgaacg caatgctgct ggagatgctt
     1501 gcccgcaccc ggccctgagg aggaacgcca atgtccgacg ccagcgcgat cgaacgcgga
     1561 ccgggcgaga tgctgggcaa tgccgggccc ggcatgctgg ccggcctgcg cgtgatcgaa
     1621 gtcgccgacg agcgcgccga atacgccgga ctgctgctgg ccgggctggg cgccgaggtg
     1681 atcaagctgg aaccgccgga gggcaacgcc acgcgccgca tcggcccgtt cctggacgac
     1741 cagcccggac ccgaccgctc gctgttcttc tggcactata accgcaacaa gaaatccgtg
     1801 gtgctggact ggcgtgatcc ggcggcgcgc gaccggatgc tgcagctgct ggacggcgcc
     1861 gacgtgctgc tggactcctc ctgcggcgca ctgaaccagg tgctcggcct ggaccgcgcc
     1921 gcgttgggcg cgcgctttcc gcggctggtc accgcacgca taacgccgtt cggggacgac
     1981 ggtccctggg cggatttcaa aggctccgac ctgatccatc tggcgctggg tggcgtcatg
     2041 atgaactgcg gctacgaccc cgatccgcgg ctggaatacg acacaccgcc gatcgcgccg
     2101 cagatctggc acgcctatca catcgccggc gagcagcttg ccaccggcat catcgccgcg
     2161 ctgatccatc gccattgcag cggcgagggc caggacgtgt ccatcgccat ccatgaagcg
     2221 gtgtcgaaga atcccgaact cgacatcatg cactgggtga tgcgccgcgt gccgctatgg
     2281 cggctgacca accggcatgc ggtggaaacg ccgaaccact cgccgtccat ctgccatacc
     2341 aaggacgggc gctggttcat catgcacggc atgggcgcgc gcgacctgaa gaacctggtg
     2401 ccgctgctga gcaaatacgg catgcaggcc gacctgcagc caccgccccc ggatgccgat
     2461 ttgaaggcgc gcaacgtgcc cggcagccag gccggcgacg aggcgcgcgc gcacatgctg
     2521 gacgtggtgc agcgcttcgt gcgcgcctgg acctatgccg acatgccgtg gcgggaggcg
     2581 caggatgccg gcctgctctg ggcgccgctg cgcaagccgc atgagaacgc gctcgacgag
     2641 cactggctga aacgcaagag cttcgccgac gtgcagcatc cggagctggg ccgcagcttc
     2701 cgctatccca ccagcaaatg gctgtcgaac cagaccagct ggcaggtggg ccggcgcgcg
     2761 ccgctgctgg gcgaagacac gcaagcggtg ctgcgcgaac cgacgcggcg gcccagcgtg
     2821 ccggcgcagc cgcgcggggt ggcgaatccg gtgcgttcgg cgctgcacga caagccgttc
     2881 ccgctgcaag gcgtgaagat cctggatttc gcctggttcc tggcatcagc cggcggcaca
     2941 cggttcctcg cgggcctggg tgcggaaagc tacaaggtgg agtggaagga caatcctgat
     3001 acgcgccttg ccgcgatggc accggtgggc ggccgcgcgg cacgcgatgc ggcgaccggg
     3061 tcgctgccgg gcgtgaaaga ccacgacatg ggcgggcagt tccacaacaa gaacgccggc
     3121 aagcgtggga tttcgctgaa catccggcat ccgaaggggc tgcagatcgc caaggacctg
     3181 gtgcgcatct gcgatgtggt cgccgagggg ttttcacccg gcgtgttgca gcgcctgggc
     3241 ctcggctacg acgtgatgaa gagcatccgg ccggacatca tctacatcca gcaggccggc
     3301 atgggcgcac atggcacgta tggccggatg cgcaccgtcg gccccgtcgc cgccgccttt
     3361 gccggccagg gcgacatgtc cgggctgccg gaaccggcaa tgccggtcgg ttggggctat
     3421 tcctatctcg actggatggg cgcctacggc tacgcgctgg cactgctcgg cgcgctgtac
     3481 caccgcgagc gcaccggcga ggggcagtgg atcgatgcgt cgcagtgcga atcgggattg
     3541 ttcctgaccg gcaccacgat tctcgactgg tgggcgaacg ggcgcgaatg gcggcgctac
     3601 ggcaaccgct cgccatacaa gccggcggcg ccgcacggtg cgtatcgctg cgccggcacc
     3661 gaccgttgga tcgccattgc ctgcttcacc gatgccgagt ggcaggcatt ggcgcaggtc
     3721 gcgggccaca cagcgtggct ggaagaccgc cgctttgcca cgctggcgga tcggctggcg
     3781 caccaggacg cgctggacgc gacggtggaa tcctggacgc gcacgcagga gcctcatgcc
     3841 tgcatgctgg cgctgcaacg cgccggcgtg ccggccggcg tgtgccagaa cgcggaagac
     3901 cgctgcgacc acgatccgca actgcgccac ctgaactggc tgaccgaggt caccggcagc
     3961 aagatcggca cctggccggt gtacgagttg ccgatgaaac tctcccatac acccgcctat
     4021 gtcgggggcc cgatcaatcg cggcgcaccg tgctatggcg aggacaacga atggctgctc
     4081 acgaacctgc tcggcatgag ccggtccgat gtacagcggc tggcggaaga aggcgtgata
     4141 tgatgggacg acgtcacccc gtcatggccg gcgaaggccg gtcatccacg acttcgtcac
     4201 gcggaacgcg tcgaactcga tgtacttggg tcggagcgtc ggcattcgaa ggcctggatc
     4261 gcggccttcg ccgtcatgac ggggggcttt gccagttttg ttgacgccgg ctgaatgcga
     4321 cgcaacggca cgggagatac gcatgccagg cgaacctgca atcgaccacg tcacggtcaa
     4381 tggccggacc tatgccgtgc cgcaatcacc ggtcgtggtg gtttgcttgg atggctgcga
     4441 tcccgagtac ctcgcggcgg ggttcgcggc caatgaactg cccgtcttcg cccgcatcgc
     4501 acgcgacggc ttcgccagtg aatcgctgtc ggcgctgcca agcttcacca acccgaacaa
     4561 ctgctcgctg gtaaccgggg ttccgccatc cgtccatggc gtgtccggca atttctatct
     4621 ggaccggcac agcggcgaga cggtgatggt caccggcgac aaggaattgc aggcgcccac
     4681 catcctcgcg gcgatggcgc agaccggcgt gccgaccgcc gtcgtcaccg cgaaggacaa
     4741 gctgcgcgcc gcactcgcca aaggactgcc aattggcgcg aatggcattg ccttctccgc
     4801 gcagaacgcc gccgcagcgc gggaacagac gcacggcatc ggcaatgtga cccgactggt
     4861 cggccgcggc gaacccgacc agtactccgc cgatctgtcg ctgttcgtgc tcgatgccgg
     4921 tgtgcggctg ctggaaaccg gacgcgcccg gctgctgtat ctctcgctgt ccgactatgt
     4981 gcagcaccgc tacgcgccgc aggaacccga ggcacgggac ttcctccgtg ccgtggacgc
     5041 gcggctgggg cgcatgatgg atctcggagc catcgtcgcc gccacggccg atcacggcat
     5101 gcacgacatg gcgaaacccg acggcacccc gaatgtgatg ttcgtcggcg atgtgctcga
     5161 cgccgccttc ggccccggtt cctgccgggt gatctgcccg atcaccgatc ccttcgtccg
     5221 ccaccacggc gcgctgggcg gccttgtgcg ggtctatgtt gccgccgggg ggcccgcgat
     5281 ccgcgccgtc accgcccatc tcgccgatct gccgggcatc gaccaggcat tggaaggcag
     5341 cgaagcctgc cgccgctacg atctcg
//
LOCUS       DASSZH010000698         6433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1380610, whole genome shotgun sequence.
ACCESSION   DASSZH010000698 DASSZH010000000
VERSION     DASSZH010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6433
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380610"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..1343
                     /locus_tag="VFN42_15045"
     CDS             183..1343
                     /locus_tag="VFN42_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HET8997984.1"
                     /translation="MASAHGLSLHSFRTESGDSEHYIAIEAPPQLSLGRQIDAVAQRY
                     AEALQSLRLSPDSAVFRRIYLSDAANQAALVRDSSLYREPLDSPVAVSVVQQPPLSGA
                     KLAMLAYHIEAAAPLAKSRTGAGDLLLQKGALRHLWTTQLCTANTAAPSSAAEQTRDV
                     FERLIGALDGQGGTLADNCVRTWIYVKDVDVFYQDMVAARSALFAQHGLTRDTHYLAS
                     TGIEGTCSHRYDVVLMDAYSIIGLAPEQVSYLNAYDRLCHTRDYNVTFERGTRIAYAD
                     RAHLLISGTASIDPAGHVMHCGDVGRQLARAMGNVDALLRAGGGSIEAMSHFIIYLRD
                     PSDHDVVEACFAESFPGVPRLILRGPVCRPEWLVEVEGVAMVPNDAPALPAF"
     gene            1402..2091
                     /locus_tag="VFN42_15050"
     CDS             1402..2091
                     /locus_tag="VFN42_15050"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HET8997985.1"
                     /translation="MADSDAPFTMLDETAIAAAELRRDPYDFCFVENAMPQRLKDEVL
                     ADVPTIPDRGSYALPDLQYGTRFGAVVQDLMSARFRHLVEEKFDIDLSKRPACIVMMG
                     NTTGHYNEGYAHPDSPHKIITVLVGFSRAWPYERGRLRVLRSNDREDCAFEFAPEFGR
                     MLMFRVCDHSWHGFLPQKGQRMSLQLCYVDSEWYVRKEYARHSLSAYAKSVPLLRKAI
                     DWAPRRMPGAR"
     gene            2091..2768
                     /locus_tag="VFN42_15055"
     CDS             2091..2768
                     /locus_tag="VFN42_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HET8997986.1"
                     /translation="MQYPPVPAVDAIAEHFMRAVRESVRADWPYRHWKLRNVLPLDLC
                     TGILTLPIAPPVLGSTDGTRNSYNDSRCFITPELRRKFPTCEALTQALQRPDVAALMA
                     ETCNIQVAGGYLRMEYIQDLDGAWLEPHRDIKEKLFSMVIYLFIGPDAAEWGTDIYDA
                     DKRWVGRSSGEFNSGTIFVAGPATWHGFDPRPILGVRRLMEINYVSPDWRDRQQLAMP
                     EAAIRLD"
     gene            complement(2790..2993)
                     /locus_tag="VFN42_15060"
     CDS             complement(2790..2993)
                     /locus_tag="VFN42_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997987.1"
                     /translation="MNAIANQAKRAPPQSPADPEQFAPGAEHQRARRAARRGLLWLGL
                     IYLGFLGGGVGIAVDQIVRPLGL"
     gene            3362..4045
                     /locus_tag="VFN42_15065"
     CDS             3362..4045
                     /locus_tag="VFN42_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997988.1"
                     /translation="MLSRIFPVVAALGVLTLSTAAMAVTIDATDSLYDAAGGTTYDST
                     GTSPVLINIPTGATFVTFSVSGTISMDSGTHKNDPDGIGAQQGLTTNPWNNISGITLP
                     WAGSLGGLFLSNSSPGSPAPASLDYTTLGTGNASYTPLIAQTFFIGDGLTGDGAGSTQ
                     IFYIPTGATRLYLGYADACNYGGNPGCYNDNTGSVSASYAFDAPEPAPFMVLATALVG
                     LAVARRRRV"
     gene            complement(4145..4663)
                     /locus_tag="VFN42_15070"
     CDS             complement(4145..4663)
                     /locus_tag="VFN42_15070"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET8997989.1"
                     /translation="MSTAPVEVKKTPAPAQTPDAWRSFRTEMDRLFDRFAGGFGFPSL
                     SRMFDGGNGGVETAFSVPSPAVDITEDDAAYKLSAELPGMAETEIEVSVVDDTLTIKG
                     EKKQETEKQEKNYYLSERSYGSFQRSFALPDSVDAEKIGAEFAKGVLTVTLPKKPEAK
                     VEAKKIEVKPAA"
     gene            4852..5664
                     /locus_tag="VFN42_15075"
     CDS             4852..5664
                     /locus_tag="VFN42_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein"
                     /protein_id="HET8997990.1"
                     /translation="MDQQTSPSQALAALRARIAHLEHGSRQRRRAALPLLPAIDAVLP
                     EGGLALGALHEIVSAGPDIEHGAAAALFIAGLLGRQRGPVLWVLEHADLFAPGLAGAG
                     LPPDRVIYAEAGKPALVLQSMEEGLRHPGLAGVVGEVSGRLSLTDTRRLQLAAEGSGV
                     AAFALRRSRRHDDPVLAEPSAAATRWRIAALPSPPALPHAPETPGLGRQRWRLDLLRC
                     RGSESGSWMVEACDAKGRLGLVADPAHRPAAPERPRAARTRSPGHAAGDIAA"
     gene            5546..>6433
                     /locus_tag="VFN42_15080"
     CDS             5546..>6433
                     /locus_tag="VFN42_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HET8997991.1"
                     /translation="MRRVVSVWLPTLPTDRLRRSGRVPPEHAPPGMPLVTSLHDGRWL
                     VVASVDTTAAGQGLYAGMPLAQAQALVPGLAIHPAEPEADAASLRDLSAWCLRYAPLT
                     AAAPPDGIWLDVTGCAHLHGGETRLLRDLTSRLAAQGFAAQAAIADTPGMAHALARYG
                     SAGGEVNHGEANHVVADEGPPPTTGCGHTTGCGHTTGCGHTTGCGHTHAAKHAKGESR
                     GWRAFARHDGGPRHDGGPDGAEHLPIVALRLPADTLAALRLLGLERIGQLQVLPRAPL
                     TRRFGPLVALRLDQLQGAVL"
BASE COUNT         1045 a         2175 c         2117 g         1096 t
ORIGIN      
        1 cccccctgcc gccaggggcc gccgggggag gggagccggc cgctgatgcg cttcctgtga
       61 cctttgatcc agatcaaaga ctaccggtca acttcgcccc tagtacggct gccatgccgg
      121 ggctgcggaa acacccgatt cgcaagccgc tcccctggct cccatgaagg ggagtggccc
      181 tggtggcttc cgcacacgga ttgtccttgc acagcttccg caccgaatcc ggcgatagcg
      241 agcactacat cgccatcgag gccccgccgc agctcagcct cgggcggcag atcgatgccg
      301 tggcgcagcg ctacgccgag gcactgcaga gcctgcggct ttcccccgac agcgcggtgt
      361 tccggcggat ctacctgagc gacgcagcca atcaggccgc cctggtgcgg gacagcagcc
      421 tctatcggga acccctggac agcccggttg ccgtctccgt cgtgcagcag ccgccgctga
      481 gcggggccaa gctggctatg ctggcctatc acatcgaagc cgccgccccg ctggcgaagt
      541 cccgcaccgg ggccggcgac ctgctgctcc agaagggcgc gctgcggcat ctctggacca
      601 cccagctctg caccgccaac accgccgccc ccagctcggc cgccgagcag acgcgcgacg
      661 tgttcgagcg gctgatcggc gcattggacg ggcagggggg cacacttgcc gacaactgcg
      721 tccgcacctg gatctacgtg aaggacgtgg acgtgttcta tcaggacatg gtggccgcac
      781 gcagcgcgct gttcgcgcag cacggcttga cccgcgacac ccattacctg gccagcaccg
      841 gcatcgaggg cacctgctcc caccgctacg acgtcgtgct gatggatgcg tattccatca
      901 tcggcttggc gccggagcag gtatcctacc tcaatgccta tgaccggttg tgccatacaa
      961 gggactacaa cgtcaccttc gagcgcggca cccgcatcgc ctatgccgac cgcgcgcacc
     1021 tgctgatctc cggcaccgcc agcatcgatc cggccggcca cgtgatgcat tgcggcgacg
     1081 tggggcggca gcttgcgcgt gcgatgggca acgtcgatgc gctgctgcgt gccggcggcg
     1141 gcagcatcga ggcgatgagc cacttcatca tctatctgcg cgatcccagc gaccacgacg
     1201 tggtggaggc gtgtttcgcc gaaagcttcc ccggcgtgcc gcggctgatc ctgcgcggcc
     1261 cggtctgccg ccccgaatgg ctggtggagg tcgaaggcgt cgccatggtg ccgaacgacg
     1321 ccccggcatt gccggccttc tgaaccaccc gcgcggccgt gaccgcccgt ccaatccgtt
     1381 tcacatgaaa ggaattcccg gatggccgat agcgatgctc cgttcaccat gctcgacgaa
     1441 accgcaatcg ccgccgccga gctgcggcgc gatccgtatg atttctgctt tgtcgagaat
     1501 gcgatgccgc agcggttgaa ggacgaggtc ctggcagacg tcccgaccat cccggatcgg
     1561 ggcagctacg cgctgcccga cctgcaatac gggacgcgct tcggtgccgt ggtgcaggat
     1621 ctgatgagcg cccgcttccg ccatctggtc gaagagaaat tcgacatcga cctgagcaag
     1681 cgccccgcct gcatcgtgat gatgggcaac accaccggtc attacaacga gggctacgcg
     1741 catcccgaca gtccgcacaa gatcatcacc gtgctggtcg gcttctcccg cgcatggccg
     1801 tatgagcgcg gccggctgcg cgtgctgcgc agcaacgaca gggaggattg cgccttcgaa
     1861 ttcgctccgg agttcggccg catgctgatg ttccgcgtgt gcgaccactc ctggcacggc
     1921 ttcctgccgc aaaagggcca gcgcatgagt ttgcagctgt gctacgtcga ttccgagtgg
     1981 tacgtgcgca aggaatacgc gcgccacagt ctcagcgcct atgccaagtc ggtgccgctg
     2041 ctgcgcaagg cgatcgactg ggcgccgcgc cggatgccgg gggcacgctg atgcaatacc
     2101 ctccggttcc cgcggtggac gccatcgcgg aacacttcat gcgggcggtg cgggagtcgg
     2161 tgcgcgccga ctggccgtac cggcactgga agctacgcaa cgtgctcccg ctggacctgt
     2221 gcaccggcat cctgacgttg ccgatcgcgc ccccggtgct cggcagcacc gacggcacgc
     2281 gcaacagcta caacgactcg cgctgcttca tcacgccgga gttgcggcgc aagtttccca
     2341 cctgcgaagc gctgacccag gcgctgcaac gcccggatgt cgcggcgctg atggcagaga
     2401 cctgcaacat ccaggtcgcc ggtggctatc tgcggatgga gtacatccag gatctggacg
     2461 gtgcctggct ggaaccgcac cgcgacatca aggagaagct gttctccatg gtgatctacc
     2521 tgttcattgg ccccgatgcg gcggaatggg gcaccgatat ctatgacgcg gacaagcgct
     2581 gggtggggcg gtcgtccggt gaattcaatt cgggcaccat cttcgttgcc ggtccggcca
     2641 cctggcacgg cttcgatccg cggccgatcc tcggcgtacg gcggctgatg gagatcaact
     2701 acgtcagccc ggactggcgc gaccggcagc aactggcgat gccggaggcg gcgatacggt
     2761 tggattgagc gtgcggaacc cggcgtggcc tacaggccaa gcgggcgcac gatctggtcc
     2821 accgcgatac ccacgccgcc gccaaggaag cccagataga tcagccccag ccacagcagc
     2881 ccgcgccggg cggcgcgccg cgcgcgttga tgttcggcgc cgggggcgaa ctgttccggg
     2941 tcggctgggg attgcggggg ggcgcgtttg gcttggttgg cgatcgcgtt catggctacc
     3001 tgggcagggg aatcctcact gagccgcaag aatatcgcag ccgatatgga aaatacaaga
     3061 tattccgcag tttagacggc gaaactgcga aaagcgccgg gtcgtgaagc cgcaaacccc
     3121 ggaggccagg gcaggggggc aaccggtgcc ggccggagct ccaggcgcca cggcggcttg
     3181 ggtcgggcgg aacagccgaa atttcgttgc gaaacccatg atgaatcgtc aaaaatcctt
     3241 acatcattcg tatttagcat cgaaacgcac ggcggtaagc ctctggaaag accgaaaaat
     3301 tgaaagtggc attacgattg catctcctgt ggcaggacga cgtccaacga gggagacttc
     3361 attgctaagc cggatttttc ctgttgttgc tgctctggga gtgctgacac tctccaccgc
     3421 ggccatggcg gtgacgatcg atgccaccga cagcctctac gatgccgcag gcggaacgac
     3481 ctatgacagt accgggacgt caccggtcct gatcaatatc ccgacgggag caacgttcgt
     3541 cacgttcagc gtctcgggta cgatctcgat ggactctgga acgcataaaa acgatccgga
     3601 cggtatcggc gcgcaacaag ggctgacgac caacccctgg aacaatattt ccgggatcac
     3661 gcttccttgg gccggttccc taggcggact gttcctgagc aattcatcgc ctgggtcgcc
     3721 ggcgccggca tcactggact acacgacgct cggcacgggc aacgcctcct acacgccgct
     3781 gatcgcccag acattcttca ttggtgacgg actgaccggc gacggcgcgg gaagcacgca
     3841 gatattttat atcccgaccg gcgccacccg gctttatctg ggctacgccg acgcctgcaa
     3901 ctatggcggc aatcctggct gctacaacga caacaccgga agcgtatcag cgagctacgc
     3961 cttcgacgcg ccggagccgg ctccattcat ggtgctcgcc accgccttgg tgggcctggc
     4021 tgtcgcgcgg cgccgcagag tctgaggggg catgccccgt catggccggc gtttcggtca
     4081 tgacggggca ggtgccgttt ccatgcaggc gccatagggc gtagcgccgg cggcgcggcg
     4141 tgcgtcaggc cgccggctta acctcgatct tctttgcttc cactttcgct tcgggcttct
     4201 tgggcagcgt caccgtcagc acgcccttgg cgaattcggc gccgatcttc tcggcatcca
     4261 cgctgtccgg cagcgcgaag ctgcgctgga acgaaccata ggagcgctcg gagaggtagt
     4321 agttcttctc ctgcttctcc gtctcctgct tcttctcgcc cttgatggtc agggtgtcat
     4381 cgaccaccga cacctcgatc tcggtttccg ccatgccggg cagttcggcg ctcagcttgt
     4441 aggcggcatc gtcctcggtg atgtccaccg cggggctcgg cacgctgaac gcggtctcca
     4501 ctccgccatt gccgccgtcg aacatccggc tcagcgacgg gaaaccgaag ccaccggcga
     4561 accggtcgaa cagccggtcc atctcggtgc ggaaggaacg ccaggcatcc ggtgtctgcg
     4621 ccggggccgg ggtctttttc acttccacgg gtgcagtgct catcgaaacc ctcctcggag
     4681 ttgctccgcg aatctacggg cgggactctg cgtcggtgag gggatgcggc gcattgacgc
     4741 aaatcaatta tgcttcgccc ggtggaggcg ccggctggcg gatttgcgac tcgactcggg
     4801 ggtatagttc tttatatgtt cctgttcccc gcacgaacgc cgggtggaca catggaccag
     4861 cagacctctc catcgcaagc cctggccgcg ctgcgtgcgc gcatcgccca tcttgagcac
     4921 ggctcccgtc agcgtcgccg cgccgccttg ccgctgttgc cggcgatcga tgccgtgctg
     4981 ccggaaggcg ggctggcgct gggcgccctg cacgagatcg tgagcgccgg ccctgatatc
     5041 gagcatggcg ccgccgccgc gctgttcatc gccgggctgc tgggccgcca gcgcggcccg
     5101 gtgctgtggg tgctggagca tgccgacctg ttcgcccccg gcttggccgg ggccggcctg
     5161 cccccggacc gggtgatcta tgccgaggcc ggcaagcccg ctttggtgct gcaaagcatg
     5221 gaggagggcc tgcgtcatcc gggtctggcc ggggtggtgg gggaggtgtc cgggcggctc
     5281 agcctgaccg acacccgccg gctgcagctc gccgcggaag gttccggcgt ggccgccttc
     5341 gccctgcggc gcagccggcg gcatgacgac ccggtgctgg cggagccctc ggctgcggcc
     5401 acccgctggc gcatcgccgc cctgccgtca ccgcccgctt tgccgcatgc gccggaaacg
     5461 cccgggctgg gccggcagcg ctggcggctg gacctgctgc gctgccgtgg cagtgaatcc
     5521 ggatcttgga tggtggaggc gtgcgatgcg aagggtcgtc tcggtctggt tgccgaccct
     5581 gcccaccgac cggctgcgcc ggagcggccg cgtgccgcca gaacacgctc ccccgggcat
     5641 gccgctggtg acatcgctgc atgacggccg ctggctggtg gtggcgtcgg tggacaccac
     5701 cgcggccggg caggggctgt atgccggcat gccgctggcc caggcgcagg cactggtgcc
     5761 cggactggcg atccaccccg ccgaaccgga agcggatgca gcgtcattgc gcgacttgtc
     5821 cgcctggtgc ctgcgttatg cgccactgac cgctgcggcg ccgccggacg ggatctggct
     5881 ggacgtcacc ggctgcgccc acctgcatgg cggagagacg cggctgctgc gcgatctgac
     5941 cagccggctg gccgcccagg gctttgccgc ccaggccgcg attgccgata cccccgggat
     6001 ggcacatgcg ctggcgcggt atggttctgc cggcggtgag gtaaaccacg gcgaagcaaa
     6061 ccacgtcgtg gcggacgaag gcccgccacc cacgactggc tgcggacaca cgactggctg
     6121 cggacacacg actggctgcg gacacacgac tggctgcgga cacacccacg ctgcgaagca
     6181 cgccaaagga gaaagtcgtg gatggcgggc cttcgcccgc catgacggtg ggccccgcca
     6241 tgatggtgga cccgatggcg cggaacacct ccccatcgtc gcgctgcgtc tgcctgccga
     6301 cacattggcc gccctccgcc tgctcggact ggagcgcatc ggccagctgc aggtcctgcc
     6361 gcgcgcgccg ctcacccgcc gtttcggccc cctggtcgcg ctccgtctgg accagttgca
     6421 aggcgcggtg ttg
//
LOCUS       DASSZH010000699         3078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_510835, whole genome shotgun sequence.
ACCESSION   DASSZH010000699 DASSZH010000000
VERSION     DASSZH010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510835"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="VFN42_15085"
     CDS             complement(<1..1025)
                     /locus_tag="VFN42_15085"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HET8997992.1"
                     /translation="MSAPTGRLMLYGNIGPELYHYDVDVDAGTLTRRSAVTLPANVQY
                     AWPHASRRYLYVATSDGAPGGGAPGSRHHLNALRIDPASGALAPHGDPVPLPSRPIHM
                     STDIPSAHALVAFNNPSALRVYRINADGTAGAEVAQPEPVDAGIFAHQVRVTPDNRQA
                     ILVARGFDAEAGTPEQPGSLKLFDYADGVLRNEASIEPDGGYGFGPRHLDFHPTRPWV
                     FVSLERQNALDLFTLEHGRLSPAPLFRKNTLARPHEKPGRQVVSTVHVHPNGRFVYVG
                     NRASDATVVNGKRVFVGGENSLAVFAIDPATGEPKLLQIEDTRGIHPRTFHIDPSGRL
                     LVVAHIMG"
     gene            1125..1613
                     /locus_tag="VFN42_15090"
     CDS             1125..1613
                     /locus_tag="VFN42_15090"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HET8997993.1"
                     /translation="MHDDLHWMRRALELARQGEAREGANPIGCVIVRDGALLAEGYNE
                     VDLRHDPTAHAEMVAIRRAGASLQCHELRGATLYSTLQPCGMCTMATIWAKIGRVVFG
                     AGRHDVHAMYFEDRHLDTLDFIRDAYRDDLVLQGGLLAADCARLYVPPDAVVPREEQF
                     NR"
     gene            complement(1603..2112)
                     /gene="gpt"
                     /locus_tag="VFN42_15095"
     CDS             complement(1603..2112)
                     /gene="gpt"
                     /locus_tag="VFN42_15095"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014104823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HET8997994.1"
                     /translation="MTAHYYTVTWDQLHRDGRALAWRLIERGPFHGVVAITRGGLIPA
                     AIIARELDIRLVETVSVLTYGGARTGTLENERTIGDPVVLKPPAAAGNGEGFLLVDDL
                     VDTGTTARLVRKLLPRAHFACIYAKPAARDVVDSFVTEVSQDTWILFPWDTEPQFVAP
                     LAKRGALSG"
     gene            complement(2294..>3078)
                     /locus_tag="VFN42_15100"
     CDS             complement(2294..>3078)
                     /locus_tag="VFN42_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8997995.1"
                     /translation="CVRWRFSAKPFPARTSPMFPHARLRRHATVGTMLLALLGVMGAA
                     QAAPVPAGSSQQAQGAATQDQQGQSHTKGQGQSHATGQQASPQAQASANAIGQHGTRA
                     PVGLSPGPKAAGATAENPTPHAPPGKQVVPGSENPAGPASPRRTTLTGATAANAGPTG
                     AVGSSFSHHRIELPGTGAEAQTGPGTKLARQALPYRQHSQAASNPNGYNPAELAPASN
                     APQPPAQGYHPSQVAGVGQQAPARTGGKASHPAGTGGVQSGG"
BASE COUNT          414 a         1087 c         1091 g          486 t
ORIGIN      
        1 cccatgatat gcgccaccac cagcagccgc ccgctgggat cgatatggaa ggtgcgcgga
       61 tggatgccgc gcgtgtcctc gatctgcagc agcttcggct cgccggtggc cggatcgatc
      121 gcgaacaccg ccagcgaatt ctccccgccg acaaacaccc gcttgccgtt caccacggtg
      181 gcgtcgctgg cgcggttgcc gacatagacg aagcggccgt tcggatgcac gtggaccgtg
      241 ctgaccacct gccgcccggg cttttcgtgc ggccgagcca atgtgttctt gcggaacagg
      301 ggagcgggcg agaggcggcc gtgctccagc gtgaacaggt ccagcgcgtt ctgccgctcc
      361 agcgacacga agacccaggg gcgagtcgga tggaaatcca ggtggcgcgg gccgaacccg
      421 taaccgccat ccggttcgat ggaggcctcg ttgcgcagca cgccgtccgc gtagtcgaac
      481 agcttcagcg aaccgggttg ttccggcgtg ccggcttccg cgtcgaagcc gcgcgccacc
      541 aggatcgcct ggcgattgtc cggcgtgacc cgcacctggt gcgcgaagat gccggcatcc
      601 accggctcgg gctgcgccac ctccgcaccg gcggtgccgt ccgcgtttat ccggtacacg
      661 cgcagcgcgc tgggattgtt gaacgccacc agcgcatgcg cggatgggat gtccgtgctc
      721 atgtggatcg gccggctcgg cagcggcacc ggatcgccat gcggcgccag cgcaccgctg
      781 gcgggatcga tgcgcagcgc attgaggtga tggcgcgatc cgggcgctcc cccgcccgga
      841 gcgccgtcgc tggtcgccac atacagatag cggcgggaag cgtgcggcca ggcgtactgc
      901 acattggccg gcagcgtcac cgcgctgcgc cgggtcagcg taccggcgtc cacatcgacg
      961 tcgtagtgat acagttccgg accgatattg ccgtacagca tcagccggcc ggtgggcgcg
     1021 gacatggcag tctcccgtgc aagcctgccg ccgatcctgc ggcagccggc gcacgccgcc
     1081 aagcgtgatg cccctgtggc ggcgcacgcc gctacactcg gcggatgcac gacgatctgc
     1141 actggatgcg ccgcgcgctg gagctggcgc ggcaaggcga ggcacgcgaa ggcgccaacc
     1201 cgatcggctg cgtcatcgtg cgtgacggcg cgctgctggc cgagggctac aacgaggtgg
     1261 atctccgcca cgatcccacc gcgcatgccg agatggtggc gatccgccgc gccggcgcgt
     1321 ccctgcagtg tcacgaactg cgcggcgcca cgctgtacag cacgctgcag ccctgcggca
     1381 tgtgcaccat ggcgacgatc tgggcgaaga tcggccgcgt cgtgttcggc gccggccggc
     1441 acgacgtgca tgcgatgtat ttcgaggacc gccacctgga cacgctggat ttcatccgcg
     1501 acgcgtatcg cgacgacctg gtgctgcagg gcggcttgct ggcggccgat tgcgcgcggc
     1561 tttatgtgcc gccggatgcc gtcgtgccgc gcgaggagca gttcaaccgc tgagtgcgcc
     1621 gcgcttcgcc agcggggcaa cgaattgcgg ctcggtgtcc caggggaaca ggatccaggt
     1681 atcctgcgac acctcggtga cgaagctgtc caccacgtcg cgcgccgccg gcttggcgta
     1741 gatgcaggcg aaatgcgccc gcggcagcag cttgcgcacc agccgcgccg tggtgccggt
     1801 gtccaccaga tcgtccacca gcaggaaccc ctctccgtta cccgcggcag ccggcggctt
     1861 cagcaccacg ggatcgccga tggtgcgctc gttctccagc gtgccggtgc gcgcgccgcc
     1921 ataggtgagc acggacaccg tctccaccag ccggatgtcg agttcgcgtg cgatgatcgc
     1981 cgccgggatc agcccgccgc gggtgatcgc caccacgccg tggaagggac cgcgttcgat
     2041 caggcgccag gccaaggccc gtccatcgcg gtgcaactgg tcccaggtga cggtgtaata
     2101 atgcgctgtc atggctgcat cctgcggatt gcccgccgcc ggcgcaagca gccatgatcc
     2161 ggcacagccc tcggattggc gcaggcgttg ccattgccgt tgatgccgcc gcgattgccg
     2221 cccggcatga ccgttgccga tacgtcgcgc tggcgttcgc agttgctcgg caatgagcgc
     2281 cggcgcggtt ccgctatccg ccgctctgca cgcccccggt gcccgccgga tgggacgcct
     2341 tgccgccggt ccgcgccggc gcctgctgcc ccacaccggc aacctgcgac gggtggtagc
     2401 cctgcgcggg cggctgcggg gcgttgctgg ccggcgcgag ttccgccgga ttgtagccgt
     2461 tgggattgct ggccgcctgg ctgtgctgcc ggtacggcag cgcctgccgg gccagcttgg
     2521 tgcccgggcc ggtctgcgcc tccgctccgg tgccgggcag ttcaatccgg tggtgcgaaa
     2581 agctgctgcc caccgcgccg gtcgggccgg cattcgccgc cgtggcgccg gtcagtgtgg
     2641 tgcgcctcgg gctggcgggc ccggccgggt tttccgaccc tggcaccacc tgcttgccgg
     2701 gcggcgcgtg cggcgtcgga ttctccgcgg tggcgccggc agccttgggg ccgggcgaca
     2761 gccccaccgg cgccctggtg ccgtgctgtc cgatcgcatt cgcgcttgcc tgtgcctgcg
     2821 gcgacgcctg ctgccccgtg gcgtgcgact gcccctgccc cttggtgtgc gactgcccct
     2881 gttggtcctg cgtggcggcg ccctgcgcct gctggctgga gccggcgggc accggcgcgg
     2941 cctgcgccgc tcccatcacc ccgagcaatg ccagcagcat cgttccgacc gtggcatggc
     3001 ggcgcaggcg ggcgtgtggg aacatcgggc tggtcctggc ggggaatggc ttggccgaaa
     3061 accgccaccg gacgcagc
//
LOCUS       DASSZH010000700         3495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_238411, whole genome shotgun sequence.
ACCESSION   DASSZH010000700 DASSZH010000000
VERSION     DASSZH010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238411"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VFN42_15105"
     CDS             <1..766
                     /locus_tag="VFN42_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019086168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET8997996.1"
                     /translation="VDPAVIPLVKAAAMVERNGRDYARYLERVFRDDPDFQQAADYWA
                     DEEVQHGDALGRWASLAGPGWDYQAAFARYRDSYQLPLDAEASVRGSRTGELIARCMV
                     ETGTSSYYSALADGTDEPVLKQICRHIAADEFRHFKLFYDHMKRYLGREKIGLLRRLR
                     IALGRIGESEDDELAYAWYCGNETGTQAFQRRRCTAAYMSRAIGFYRFRHVERGMGMI
                     FKAVGLPPRGRLSDVSARMAWRLMRWRQQRLARQMG"
     gene            complement(782..1678)
                     /locus_tag="VFN42_15110"
     CDS             complement(782..1678)
                     /locus_tag="VFN42_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HET8997997.1"
                     /translation="MRDDLPVWRSLLFVPVTAEKFVRTGADRGADGIILDLEDAVAPS
                     QKEHARTLIADAIPQVNRRGADVLVRVNRPWRLFVRDLEAAVIPGVKALMLTKVDSVE
                     HVLAAAEIVAELEAERGLPAGAMQFVVLVETTAGFFRLEAIAKSHPRVVAISLGTEDF
                     TADAQMMPDPEGLFYPKQHTVFAARAAGILPMGFVGSIADFRDQDAFRAIVRRSKRLG
                     FAGASAIHPLQVAVLNEEFGPSEAEVERAQRMVDAYQAAYAQGLGAVQFEGAMIDVPV
                     VQRAKNVLATAAAISRRASARG"
     gene            complement(1846..3084)
                     /locus_tag="VFN42_15115"
     CDS             complement(1846..3084)
                     /locus_tag="VFN42_15115"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET8997998.1"
                     /translation="MLQRIEFDPTAPAPLDGVRVVDMSRLVAGNMLSLQLADQGAEVI
                     KIEDPKVGDPLRAWRIQGMSLYWKVYARNKKSLALNLRPQAGRDVLLDLLAQSQVLIE
                     NFRPGTLEQMGLAPATLHARNPKLVIVRITGFGQDGPYRERPGFGTLVEAMSGFASKN
                     GYPDRPPVLPPLALADMVSGLYGAYAVMVALRVAERGGRGQVIDLPLLDPIISILGPD
                     AAIYRTSGEKPQRTGSRSLTTAPRNVYRTRDDRYIAISASMQVMAERVFRAIGKPEMI
                     DDPRFRTNTDRVQHIDACDGAVAEYIAAHTLEENMAVFSAAEVTANPVYEIDQLLDDP
                     HVQERGVLVEAPDVEAGSVVMHNVIPRLSETPGRLRTPAPALGEHTREILGEIGMAEG
                     RIAALAADGIVKIETTDEHR"
     regulatory      3108..3325
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          574 a         1188 c         1172 g          561 t
ORIGIN      
        1 ggtcgatccc gcggtgattc cgctggtgaa agccgcagcc atggtggaac gcaatggccg
       61 cgactatgcg cgctacctgg agcgcgtgtt ccgcgacgac cccgatttcc agcaggccgc
      121 ggactactgg gcggacgagg aagtgcagca cggcgacgcg ctcgggcgtt gggcgagcct
      181 ggctggtccg ggctgggact accaggcggc gttcgcgcgc tatcgcgaca gctaccaatt
      241 gccgctggat gccgaggcgt cggtgcgggg atcacgcacg ggcgagctga tcgcacgctg
      301 catggtggag accggcacgt cgtcgtacta ctcggccctg gccgacggca cggacgagcc
      361 ggtgctgaag cagatctgcc ggcatatcgc cgccgacgag ttccgccatt tcaagctgtt
      421 ctacgaccac atgaagcgct acctggggcg cgagaagatc ggcctgctgc gccggctgcg
      481 catcgcgctg gggcgaatcg gcgagagcga ggacgacgaa ctcgcctatg cctggtactg
      541 cggcaacgag accggcacgc aggccttcca gcgccggcgc tgcacggcgg cgtacatgtc
      601 gcgggcgatc gggttctacc gcttccgcca tgtcgaacgc ggcatgggca tgatcttcaa
      661 ggcggtcggg ctgccgccgc gcggccgctt gtccgacgtc tccgcgcgca tggcctggcg
      721 gctgatgcgc tggcggcagc agcggctggc acggcagatg ggctgaaccc ctcggccggc
      781 ttcagccccg ggctgatgcc cgcctgctga ttgccgccgc ggttgccagc acgttcttcg
      841 cccgctgcac caccggcacg tcgatcatcg cgccctcgaa ctgcaccgcg ccgagtccct
      901 gcgcgtaggc cgcctgatag gcatcgacca tgcgctgggc gcgttccact tcggcctcgc
      961 tggggccgaa ttcctcgttc agcacggcca cctgcagcgg atggatcgcg ctggcgccgg
     1021 cgaagccgag gcgcttggag cggcggacga tggcgcggaa cgcgtcctgg tcgcggaaat
     1081 ccgcgatcga gccgacaaag cccatcggca atatcccggc ggcacgcgcc gcgaacaccg
     1141 tgtgctgctt gggatagaac aggccttccg ggtccggcat catctgcgcg tcggcggtga
     1201 aatcctcggt gcccaggctg atcgccacca cgcgcggatg cgacttcgcg atggcttcca
     1261 ggcggaagaa gccggcggtg gtttccacca gcaccacgaa ctgcatggcg ccggcgggaa
     1321 ggccgcgttc ggcttccagt tcggcgacga tctctgccgc cgcgagcacg tgttcgacgc
     1381 tgtccacctt ggtgagcatc aaggctttca caccagggat caccgctgct tccaggtccc
     1441 gcacgaacag gcgccacggg cggttcaccc gcaccagcac gtcggcacca cggcggttca
     1501 cctgcggaat ggcgtcggcg atcagcgtgc gcgcatgttc cttctgcgag ggcgcgacag
     1561 catcttccaa atcgagaatg atgccgtcgg cgccgcgatc ggcgccggtg cggacgaatt
     1621 tttccgcggt aacggggacg aacagcagcg aacgccagac gggaagatcg tcacgcatgg
     1681 tcaggtgtcc tcaagcaggt gaaaccacag ataaacacgg atcaacacag ataaatgccg
     1741 atgaggcgat gggacccgat cgcccccggg tagttccgcc gaaggcatgg aataacatct
     1801 gccggctgca ccacgcttcg cgacagagca tcgggccacc gggattcatc tgtgttcatc
     1861 tgtggtttca atcttgacta tcccatcggc cgccaaagcc gcgatgcgtc cctcggccat
     1921 gccgatctcg ccaaggatct cccgcgtgtg ttcgcccagc gcaggcgccg gcgtgcgcag
     1981 ccgccccggc gtttccgaca gccgcggaat cacgttgtgc atcaccaccg agcccgcttc
     2041 gacatccggc gcctctacca gcacgccgcg ctcctgcaca tgcggatcgt cgagcaactg
     2101 gtcgatctcg tacaccggat tcgccgtcac ctcagccgcg gagaacaccg ccatgttctc
     2161 ctccagcgta tgcgccgcga tgtactccgc gacggcgccg tcgcacgcgt cgatgtgctg
     2221 cacgcggtcg gtattggtgc ggaagcgcgg gtcgtcgatc atttccggct tgccgatggc
     2281 gcggaacacc cgctccgcca tcacctgcat cgaggcggag atggcgatgt agcggtcgtc
     2341 cctggtgcga tacacattgc gtggcgcagt ggtcagcgag cggctgccgg tgcgctgcgg
     2401 cttctcgccg gacgtgcggt agatcgccgc gtccggaccg agaatggaaa tgatcggatc
     2461 gagcagcggc agatcgatca cctggccgcg gccgccacgc tccgccaccc gcagtgccac
     2521 catcacggcg tacgcgccgt acagcccgga caccatgtcg gccagcgcca gcggcggcag
     2581 caccggcggc cggtccggat agccgttctt cgaggcgaag ccggacatcg cttccaccag
     2641 cgtgccgaac ccaggccgct cacggtacgg cccatcctgg ccgaagccgg tgatgcgcac
     2701 gatgaccagc ttcgggttgc gcgcatgcag cgtcgcgggc gcgagtccca tctgctccag
     2761 cgtgccgggg cggaaattct cgatcaggac ctgcgattgg gccagcaggt ccagcagcac
     2821 gtcccgcccg gcctgcggcc gcaggttcag cgccaggctt ttcttgttgc gggcatagac
     2881 cttccagtac aggctcatcc cctggatgcg ccaggcgcgc agcggatcgc cgaccttggg
     2941 gtcctcgatc ttgatcacct cggcgccctg gtcggccagc tgcaggctga gcatgttgcc
     3001 cgcgaccagg cgcgacatgt ccacaacgcg gacaccgtcc agcggcgccg gcgcagtggg
     3061 gtcgaattct atacgttgca gcattgcact ctctctcgga cgcagtagtt tgtccgccag
     3121 acgccggtgg cccgcaaggg ctgaaacggg aaagccgacc ctcggaccag atccgggggc
     3181 agggctgctg cccccgcaac tgtgcgcggc cggccttcgc atcgcggggg cggcctggtc
     3241 ccaaagtgcc attgccgcaa ggcgagaagg cggggccggg cagggccgtg cagccaggag
     3301 accggccggt gtcgagactg cgcgcgcgca tccgggcggg tgaaccgatg cagcggggag
     3361 aaacccgcag gcgagccgtc gatccagatc gatcgaggag gctcgcatgc gcatcattgc
     3421 cgcgctgctg acggtcagcg tctgtgttcc cgcgctgctg gcggtcagcg tctgtgttcc
     3481 cgcactggcc cagga
//
LOCUS       DASSZH010000701         4486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_238537, whole genome shotgun sequence.
ACCESSION   DASSZH010000701 DASSZH010000000
VERSION     DASSZH010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238537"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFN42_15120"
     CDS             <1..252
                     /locus_tag="VFN42_15120"
                     /inference="COORDINATES: protein motif:HMM:NF040519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sbal_3080 family lipoprotein"
                     /protein_id="HET8997999.1"
                     /translation="RGLQPRVVPEDTAPRACPLTTAYTARWAWDLALYLAYAELRVYR
                     DGRLAGEVRYDSSAGGLNLGKFVDARKKIEEMTGQLFAG"
     gene            265..837
                     /locus_tag="VFN42_15125"
     CDS             265..837
                     /locus_tag="VFN42_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8998000.1"
                     /translation="MRPGVHRMPSTVRGLVVLVATVMLVAACGGMHVLEDRNPDYAAH
                     TPIHTVFLVAPEDLFADQSTNLVQALGGELAKHGYTVVSTLQIASFLPGHDTSVPGVL
                     SPDSLHALVDHQVDAVLVLRTGAALMGASEIRHAKVAATSTRTQKAIGDIQWDNAWGG
                     MPGSPADHMMRRGPETVAHEIADALARLLG"
     gene            1035..2387
                     /gene="hemN"
                     /locus_tag="VFN42_15130"
     CDS             1035..2387
                     /gene="hemN"
                     /locus_tag="VFN42_15130"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014105742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HET8998001.1"
                     /translation="MDLETLLAKYDRRVPRYTSYPTAPHFSPAIDATRYADWLRALPD
                     DAALSLYLHVPFCARLCYFCACHTTVVHRPEPLISYGQAMLAEIDLIAAAIGRRLPVQ
                     HVHWGGGTPSALPAELMLAVMQRLRERFAFRPDAEVAIEVDPRELSDASLAAVGAMGA
                     TRASLGVQDFDPVVQHAINRIQDFALTADCADRLRGVGVASINLDLVYGLPHQTESGV
                     RRTVEQALQIRPDRAAVFGYAHVPWMKKHQALLPEEALPGAHERFAQRQAVEEVLVGH
                     GYLPVGLDHFALPGDSLAIAAADARLKRNFQGYTTDDAPVLLGIGSSSIGSLPQGYVQ
                     NQPSIPGWRDAVRAGMLPVSRGIALSAEDRLRRAVIEQIMCRHQVDLHEIAAAHGADP
                     VSLMDAAPMLQEMARDGLVEWDGYRVTVTTEGRPLVRSVASAFDTYLRSGAGRHSAAV
                     "
     gene            2394..3026
                     /locus_tag="VFN42_15135"
     CDS             2394..3026
                     /locus_tag="VFN42_15135"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HET8998002.1"
                     /translation="MADDPGTIDYTVFGAAKLQQVPFDHLVVPGFVPVDAAAAAAAAF
                     PGPDLPGVLPAPATPGDDGFGRLLTALRSPRTTQAFAEKFGLKLDPATLMITLRARTR
                     LADGRIHTDSTTKLVTALIYLNGDWSASGGRLRLLRGPDNLDDMIAEVPPLAGTLLAF
                     RRTDNSWHGHKPFEGVRRAVMLNWMVDAATARRELRRHAMSAGLKRLFAA"
     gene            3041..3724
                     /locus_tag="VFN42_15140"
     CDS             3041..3724
                     /locus_tag="VFN42_15140"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HET8998003.1"
                     /translation="MDMLVCDRITLDHAGAHVRRALRRAAREESPFRYWLLRSVLPRS
                     LCRAVSALPFPPPAIEDTLGRRETNNPSRIFVSAANRRRYPACETLADAFQDDATIGL
                     IEDICRISLAGSYLRIECCLDSDGFWLEPHTDIGAKLFTMLVYLSDHPDAEDWGTDIM
                     DPFGTVLGRAPGSFNSGLVFVPGSDTWHGFTRRPIRGVRRSLIVNYVKPEWRSRHELA
                     FPETPVFGD"
     gene            3797..4465
                     /locus_tag="VFN42_15145"
     CDS             3797..4465
                     /locus_tag="VFN42_15145"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET8998004.1"
                     /translation="MPELDLNDTDVFTIMQTTRSMRRLKPDPVPDALINRILQAGACA
                     ANGGNAQRWCFIVIKDKAIKQAVQVWYKRAFDETIGPRYKSNPPPPGISAERFARQHA
                     AVEYLTEHFHEAPVWIVACVDEGSATPNRWSGASIYPAVQNMLLAARALGLGSTLTTR
                     HLLYEKESEQALGLPPGVHSYAILPIGYPMGRFGPVGRGDLRQMVFGDRWGETWDGLA
                     APGD"
BASE COUNT          701 a         1508 c         1511 g          766 t
ORIGIN      
        1 agggggctgc aaccacgtgt ggttccggag gacaccgcac cgcgggcctg cccattgacc
       61 acggcttata ccgcccgttg ggcctgggat ctcgcgctct acctcgccta tgccgaactg
      121 agggtctatc gggacggtcg attggcgggt gaagtgcgtt acgattcaag cgcaggtggc
      181 ctcaatctcg ggaagttcgt cgatgccagg aaaaaaattg aggaaatgac cgggcagctc
      241 tttgctggtt gatcggagaa cccgatgcgt cccggcgtcc atcgcatgcc atccactgta
      301 cgaggccttg ttgtactcgt cgcgacggtg atgctggttg cggcctgcgg cggaatgcat
      361 gtgctggaag accgtaatcc ggactatgcc gcgcacactc cgatccacac ggtgttcctg
      421 gtggctccgg aagacctgtt tgccgaccaa agcacgaatc tcgtgcaggc gctcggtggc
      481 gaactggcca agcatggcta taccgtcgtg agcactcttc agatcgcatc attccttccc
      541 ggccatgaca cctcggtgcc gggggtactg agcccagaca gcttgcacgc gcttgtcgat
      601 caccaagtgg atgcggtgct ggtactgagg accggcgctg ccttgatggg cgcttccgaa
      661 atcaggcacg ccaaggtggc cgcaaccagc acccgcacac agaaggcgat cggcgatatc
      721 cagtgggaca acgcctgggg cggtatgccc gggtcaccgg cagaccacat gatgcgccga
      781 ggaccggaaa cggtggccca tgagattgcc gacgcgctgg cacggttgct cggctaatcc
      841 ggagaggatg atcgccgcag ccggccgcgg gggacccgaa gcccgcacca acggaaattg
      901 accagggttc aattcaccca atctccgctc aacctgcgca ccttgggcgg gagagccgat
      961 cgcgggtcgg gtttcctgcc agccggttga tccagatcaa ggcccctctg ccgggaggtg
     1021 gcaatcattc cggcatggac ctcgaaaccc tgctcgcaaa atacgatcgg cgcgtcccgc
     1081 gctacaccag ctacccgacc gcgccgcatt tctcgcctgc gatcgacgcc acgcgctatg
     1141 ccgattggtt gcgtgcgctg ccggacgatg cggcgctgtc gctgtatctg catgtgccct
     1201 tctgtgcccg gctttgctac ttctgcgcct gccacaccac tgtcgtgcac cggcccgaac
     1261 cgctgatcag ctacggccag gcgatgctgg ccgagatcga cctgatcgcc gcagcgatcg
     1321 gccggcgcct gccggtgcag catgtgcact ggggtggcgg aacgccgagc gcgctgcccg
     1381 ccgagctgat gctcgcggtc atgcagcggc tgcgggaacg tttcgccttt cgcccggatg
     1441 ccgaggtcgc catcgaggtc gatccgcgcg aactctccga cgcttcgctg gcggcggtcg
     1501 gcgccatggg tgccacccgt gccagcctgg gcgtgcagga tttcgacccg gtggtgcagc
     1561 acgcgatcaa tcgtatccag gatttcgcac tcaccgccga ctgcgcggat cggctgcgcg
     1621 gtgtcggggt ggcgtcgatc aacctggacc tggtctacgg tctgccgcat cagaccgagt
     1681 ccggcgtgcg ccgtacggtt gagcaggcgc tgcagatccg gccggatcgc gcggcggtgt
     1741 ttggctacgc ccacgtgccg tggatgaaga agcaccaggc cctgctgcct gaggaagcgc
     1801 tgcccggcgc gcacgagcgc ttcgcgcaac gccaggcggt ggaggaggtg ctcgtcggtc
     1861 acggctacct gccggtcggc ctcgaccatt tcgccttgcc cggcgattcg ctggccatcg
     1921 ccgcggcgga tgcccgtttg aagcgcaatt tccagggcta caccacggat gacgcgccgg
     1981 tgctgctggg gatcggatcg tcctcgatcg gctcgctgcc acagggttat gtgcagaatc
     2041 agccctcgat tcccggctgg cgcgacgcgg tgcgtgccgg gatgctgccg gtgtcgcgcg
     2101 gcattgccct gagcgcggaa gaccggctgc gccgcgcggt gattgagcag atcatgtgcc
     2161 ggcatcaggt cgatctgcac gagatcgctg cggcgcatgg cgccgatccc gtcagcctga
     2221 tggatgccgc gccgatgttg caggagatgg cgcgcgacgg gctggtggaa tgggatggtt
     2281 accgcgtcac cgtcaccacg gagggccggc cgctggtacg cagcgtcgcc tccgccttcg
     2341 acacctatct ccggtccggt gccggccggc attccgccgc ggtgtagcgc ggcatggcgg
     2401 acgaccccgg caccatcgat tacacggtct tcggcgcggc gaagctgcag caggtgccgt
     2461 tcgaccatct ggtcgtgccg ggcttcgtgc cggtcgatgc cgcggcggcc gcggcggctg
     2521 ctttccccgg acccgacctg ccgggcgtgc tgccggctcc ggccacgcct ggcgatgacg
     2581 gtttcggccg gttgctcact gcgttgcgca gcccgcgcac cacgcaggcc ttcgcggaga
     2641 aattcggcct gaagctggac cccgccacgc tgatgatcac gctgcgcgcc cgcacgcgcc
     2701 tggcagacgg gcgcatccac acggacagca ccaccaagct ggtgaccgcg ctgatctacc
     2761 tgaacggcga ctggtccgct tccggtggcc ggctgcgcct gctgcgcggg ccggacaatc
     2821 tggacgacat gatcgccgaa gtgccgccgt tggccgggac gctgctcgcg ttccgccgta
     2881 ccgacaattc ctggcacggt cacaagccct tcgagggcgt ccgccgggcg gtgatgctga
     2941 actggatggt ggacgccgcg acagcgcggc gggaattgcg gcggcatgcc atgtccgccg
     3001 gcttgaagcg gctgttcgca gcctgagcag gggatacgcg atggacatgc tggtttgcga
     3061 caggatcacg ctggatcacg cgggcgccca tgtgcggcgt gccctgcgcc gcgcggcgcg
     3121 ggaggagtcg ccgttccgct actggctgct gcgcagcgtg ctgccgcgct cgctgtgccg
     3181 cgcggtcagc gcgttgccgt tcccgccccc ggctatcgag gacacgctcg ggcggcgtga
     3241 aaccaacaat ccctcgcgca tcttcgtctc cgcggccaat cgccggcgtt acccggcctg
     3301 cgaaacgctg gccgacgcgt tccaggacga cgccaccatc ggtctgatcg aggatatctg
     3361 ccgcatctcc ctggccggca gctatctgcg cattgagtgc tgtctggaca gcgacggttt
     3421 ctggctggaa ccgcataccg atatcggcgc caagctgttt accatgctgg tgtacttgtc
     3481 cgatcatccg gatgcggagg attggggcac cgacatcatg gatccgttcg gcaccgtgct
     3541 gggccgcgcg ccggggtcgt tcaacagcgg cctggtgttc gtgcccggca gcgatacctg
     3601 gcacggcttc acccgccggc cgatccgcgg cgtgcggcgc tcgctgatcg tcaactacgt
     3661 gaagcccgaa tggcggtcgc gtcacgagct tgccttcccg gagacaccgg tgttcgggga
     3721 ttagcgccta tcgacaggcc ggcgacatat ggccactctc acccgcacgc aatggccacc
     3781 atgcggggag gggcggatgc ccgaactgga cctgaacgac accgacgtct tcaccatcat
     3841 gcagaccacg cggtcgatgc ggcggctgaa gccggacccg gtgccggacg cgctgatcaa
     3901 ccgcatcctg caggccgggg cctgcgccgc gaatggcggc aacgcgcagc ggtggtgctt
     3961 catcgtgatc aaggacaagg cgatcaagca ggcggtgcag gtctggtaca agcgcgcctt
     4021 cgatgagacg atcggcccgc gctacaagag caacccaccg ccgcccggca tcagcgccga
     4081 gcggttcgca cgccagcacg cagcggtgga gtacctgacc gagcacttcc atgaagcgcc
     4141 ggtctggatc gtcgcctgcg tcgatgaagg cagcgcgaca cccaaccgct ggtccggcgc
     4201 gtcgatctac ccggcggtgc agaacatgct gctggccgcc cgcgccctgg ggctcggctc
     4261 caccctgacc acgcggcatc tgctgtatga gaaggaatcc gagcaggcgc tgggcctgcc
     4321 ccccggtgtg cactcctacg cgatcctgcc gatcggctac ccgatgggcc gcttcggtcc
     4381 cgtggggcgc ggcgacctgc gccagatggt gttcggcgac cgctggggcg agacctggga
     4441 cggactcgcc gcccccggcg actgaggccc ggcgccgcgc gccctc
//
LOCUS       DASSZH010000702         2207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U704
            k141_1039682, whole genome shotgun sequence.
ACCESSION   DASSZH010000702 DASSZH010000000
VERSION     DASSZH010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968720
            Sequence Read Archive: SRR25158217
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:08:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,031
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 15 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039682"
                     /isolate="SMAG_U704"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="25.86194444 N 107.1297222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..1622)
                     /locus_tag="VFN42_15150"
     CDS             complement(126..1622)
                     /locus_tag="VFN42_15150"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HET8998005.1"
                     /translation="MREIGHFIGGKPVAGTSGRFGDVFNPASGELSARVALAGADEVN
                     KAVAAAAAAWPAWANTPPLRRARVMFKLKDLLERDRRQFSEIITAEHGKVLSDADGEV
                     QRGLEVVEFACGIPHLMKGEFTDMVGTGIDAWSIRQPLGVVAGITPFNFPLMVPLWMI
                     PVALACGNCFILKPSEKDPSAALKLAELLKEAGLPDGVFNVVQGAREAVETIIAHPDI
                     VAISFVGSTAIAETIYKGGTSHGKRVQALGGAKNHLVVMPDADLDDAVDALMGSAYGA
                     AGERCMAVSVAVAVGGVGDKLVEKLAPRVRALKIAPGTDPEAEMGPLVTREHREKVKS
                     YVDLGVKEGAKLVVDGRDLRLQGYENGFFLGGCLFDDVRTDMRIYKEEIFGPVLSVVR
                     TPDLAAATQMINAHEYGNGVAIFTGDGGAARDFTNAIQIGMVGVNVPIPVPMAFHSFG
                     GWKHSLFGDHHIYGPEGVRFYTRYKAVTQRWAGSARRGPEYVMPTLGR"
     gene            complement(1689..>2207)
                     /locus_tag="VFN42_15155"
     CDS             complement(1689..>2207)
                     /locus_tag="VFN42_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8998006.1"
                     /translation="ALVAHVADGKLSIERRSLDPAPPGDVTVTDPDGKTHKLALQASE
                     PGRSVATLPATAPGVWQVTDGKQTTYAAARAANPRELADLRATASVLGPLAHASNGGV
                     HWLGTAAHPDIPELQRTEPGRSASGGSWIGLERRHDHLVTGVSALTLLPPWAALPLLL
                     GLMVLAWRREGR"
BASE COUNT          351 a          808 c          703 g          345 t
ORIGIN      
        1 aagaatgggc gtgttccgtt gcaatccgac ccgatcgttc cggcgtggat ggccgggcca
       61 ggcccggcca tgacggggga gaagacgctc cgccttcgcc cccgcggcca cctcagccgt
      121 gtggcttagc gccccagcgt cggcatcaca tattccgggc cacggcgggc actgccggcc
      181 cagcgttgcg tcaccgcctt gtagcgcgtg tagaaccgca caccctccgg cccgtagata
      241 tggtggtcgc cgaacagcga gtgcttccat ccaccgaacg aatggaacgc catcggcacc
      301 gggatcggca cgttcacccc aaccatgccg atctggatcg cgttggtgaa gtcgcgcgcg
      361 gcaccaccat cgccggtgaa gatcgcgacc ccgttgccgt attcatgcgc attgatcatc
      421 tgcgtcgctg ccgccaggtc cggcgtacgc accacgctca gcaccgggcc gaaaatctcc
      481 tccttgtaga tgcgcatatc ggtccgcacg tcgtcgaaca ggcagccgcc caggaagaag
      541 ccgttctcgt agccctgcag ccgcaggtcg cggccatcga ccaccagctt cgccccttcc
      601 ttcacgccga gatcgacata actcttcacc ttctcgcggt gttcgcgcgt caccagcggg
      661 cccatctcgg cttccggatc ggtgcccggc gcgatcttca gcgcgcgcac gcgcggggcc
      721 agcttttcca ccagcttgtc accgacgccg cccaccgcga ccgccaccga caccgccatg
      781 cagcgctcgc ccgccgcgcc gtacgcgcta cccatcagcg catccaccgc gtcgtccagg
      841 tcggcgtccg gcatcaccac aaggtggttc ttcgccccgc ccagtgcctg cacgcgcttg
      901 ccgtgcgacg tgccgccttt gtagatcgtc tcggcaatcg cggtggagcc aacgaagctg
      961 atcgcgacga tgtcgggatg cgcgatgatg gtctccaccg cctcgcgcgc accctgcacc
     1021 acattgaaca cgccgtccgg cagaccggct tccttcagca gctccgccag cttcagcgcg
     1081 gcggacgggt ccttctccga cggcttcagg atgaagcagt tgccgcaggc cagcgccacc
     1141 gggatcatcc acagcggcac catcagcggg aagttgaacg gcgtgatgcc ggcgacgacg
     1201 cccagcggct ggcggatcga ccaggcgtcg atgccggtgc cgaccatgtc ggtgaactcg
     1261 cccttcatca ggtgcgggat gccacaggca aactcgacca cttccaggcc gcgctgcacc
     1321 tcgccatccg catccgacag cactttgccg tgttcggcgg tgatgatctc ggaaaactgg
     1381 cgacggtcgc gctccagcag gtccttcagc ttgaacatca cccgggcgcg gcgcagtggc
     1441 ggcgtgttgg cccaggcggg ccaggcggcg gccgctgcgg caaccgcctt gttcacttcg
     1501 tcggcgccgg ccagtgcgac gcgtgcggac aactcgccgg aggccgggtt gaacacgtcg
     1561 ccgaagcgcc cgctggtgcc cgcgaccggc ttcccaccga tgaaatggcc aatttcgcgc
     1621 atgtgcgtcc ctccggtttg cagcagcggt tcgataggac gatttggcac ggcttgccag
     1681 tgggggcgct agcgtccctc ccgccgccag gccagcacca tcagcccaag cagcaggggc
     1741 aatgccgccc agggcggcaa cagcgtcagc gcggaaaccc ccgtgaccag atggtcgtgc
     1801 cgccgctcca gcccgatcca cgaaccaccg gatgccgagc ggccgggctc ggtgcgctga
     1861 agctccggaa tgtcgggatg cgccgcggtg cccagccagt gcacgccgcc attggaggca
     1921 tgcgccagcg ggccgagaac gctggcggtg gcgcgcaggt cggccagctc gcgcggattg
     1981 gccgcgcgcg ccgcggcata ggtggtctgc ttgccgtcgg tgacctgcca cacaccgggc
     2041 gcggtggccg gcagcgtggc gaccgaccgg cccggctccg aggcttgcaa tgccagctta
     2101 tgggtcttgc catccggatc ggtgacagtg acgtcgccgg gcggcgccgg atcgagcgag
     2161 cgccgctcga tcgacagctt cccgtccgcg acatgcgcca ccagcgc
//
